LOCUS       DADLSA010000001       127706 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.001, whole genome shotgun sequence.
ACCESSION   DADLSA010000001 DADLSA010000000
VERSION     DADLSA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 127706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 127706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..127706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.001"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..320
                     /locus_tag="JN691_000001"
     CDS             <1..320
                     /locus_tag="JN691_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7241547.1"
                     /translation="VGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDS
                     NSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQV
                     TYP"
     gene            433..1227
                     /gene="panB"
                     /locus_tag="JN691_000002"
     CDS             433..1227
                     /gene="panB"
                     /locus_tag="JN691_000002"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAY7241548.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     ERLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1239..2090
                     /gene="panC"
                     /locus_tag="JN691_000003"
     CDS             1239..2090
                     /gene="panC"
                     /locus_tag="JN691_000003"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAY7241549.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2164..3066)
                     /locus_tag="JN691_000004"
     CDS             complement(2164..3066)
                     /locus_tag="JN691_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY7241550.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3340..3720
                     /gene="panD"
                     /locus_tag="JN691_000005"
     CDS             3340..3720
                     /gene="panD"
                     /locus_tag="JN691_000005"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAY7241551.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERSSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3724..4953)
                     /locus_tag="JN691_000006"
     CDS             complement(3724..4953)
                     /locus_tag="JN691_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY7241552.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWSQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5017..5457)
                     /locus_tag="JN691_000007"
     CDS             complement(5017..5457)
                     /locus_tag="JN691_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY7241553.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5562..6332)
                     /locus_tag="JN691_000008"
     CDS             complement(5562..6332)
                     /locus_tag="JN691_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7241554.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6329..7255)
                     /locus_tag="JN691_000009"
     CDS             complement(6329..7255)
                     /locus_tag="JN691_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7241555.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7364..8026
                     /gene="can"
                     /locus_tag="JN691_000010"
     CDS             7364..8026
                     /gene="can"
                     /locus_tag="JN691_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY7241556.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="JN691_000011"
     CDS             complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="JN691_000011"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAY7241557.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8809..11199
                     /locus_tag="JN691_000012"
     CDS             8809..11199
                     /locus_tag="JN691_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HAY7241558.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11246..12796)
                     /gene="cueO"
                     /locus_tag="JN691_000013"
     CDS             complement(11246..12796)
                     /gene="cueO"
                     /locus_tag="JN691_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HAY7241559.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVRLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12962..13309
                     /locus_tag="JN691_000014"
     CDS             12962..13309
                     /locus_tag="JN691_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAY7241560.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13415..14281
                     /gene="speE"
                     /locus_tag="JN691_000015"
     CDS             13415..14281
                     /gene="speE"
                     /locus_tag="JN691_000015"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAY7241561.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14297..15091
                     /gene="speD"
                     /locus_tag="JN691_000016"
     CDS             14297..15091
                     /gene="speD"
                     /locus_tag="JN691_000016"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAY7241562.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15129..15491)
                     /locus_tag="JN691_000017"
     CDS             complement(15129..15491)
                     /locus_tag="JN691_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAY7241563.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="JN691_000018"
     CDS             complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="JN691_000018"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAY7241564.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18618..20471
                     /locus_tag="JN691_000019"
     CDS             18618..20471
                     /locus_tag="JN691_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HAY7241565.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQASAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRLER"
     gene            complement(20542..21966)
                     /gene="lpdA"
                     /locus_tag="JN691_000020"
     CDS             complement(20542..21966)
                     /gene="lpdA"
                     /locus_tag="JN691_000020"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAY7241566.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22291..24183)
                     /gene="aceF"
                     /locus_tag="JN691_000021"
     CDS             complement(22291..24183)
                     /gene="aceF"
                     /locus_tag="JN691_000021"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAY7241567.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24198..26861)
                     /gene="aceE"
                     /locus_tag="JN691_000022"
     CDS             complement(24198..26861)
                     /gene="aceE"
                     /locus_tag="JN691_000022"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAY7241568.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27022..27786)
                     /gene="pdhR"
                     /locus_tag="JN691_000023"
     CDS             complement(27022..27786)
                     /gene="pdhR"
                     /locus_tag="JN691_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAY7241569.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28327..29700
                     /gene="aroP"
                     /locus_tag="JN691_000024"
     CDS             28327..29700
                     /gene="aroP"
                     /locus_tag="JN691_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAY7241570.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29743..30597)
                     /gene="ampE"
                     /locus_tag="JN691_000025"
     CDS             complement(29743..30597)
                     /gene="ampE"
                     /locus_tag="JN691_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAY7241571.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30594..31145)
                     /gene="ampD"
                     /locus_tag="JN691_000026"
     CDS             complement(30594..31145)
                     /gene="ampD"
                     /locus_tag="JN691_000026"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAY7241572.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31233..32126
                     /gene="nadC"
                     /locus_tag="JN691_000027"
     CDS             31233..32126
                     /gene="nadC"
                     /locus_tag="JN691_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAY7241573.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLTENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32329..32769
                     /gene="ppdD"
                     /locus_tag="JN691_000028"
     CDS             32329..32769
                     /gene="ppdD"
                     /locus_tag="JN691_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAY7241574.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32779..34164
                     /gene="gspE"
                     /locus_tag="JN691_000029"
     CDS             32779..34164
                     /gene="gspE"
                     /locus_tag="JN691_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HAY7241575.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            34154..35356
                     /gene="hofC"
                     /locus_tag="JN691_000030"
     CDS             34154..35356
                     /gene="hofC"
                     /locus_tag="JN691_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HAY7241576.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALHYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35391..36434)
                     /gene="guaC"
                     /locus_tag="JN691_000031"
     CDS             complement(35391..36434)
                     /gene="guaC"
                     /locus_tag="JN691_000031"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAY7241577.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            36659..37279
                     /gene="coaE"
                     /locus_tag="JN691_000032"
     CDS             36659..37279
                     /gene="coaE"
                     /locus_tag="JN691_000032"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAY7241578.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37279..38022
                     /gene="zapD"
                     /locus_tag="JN691_000033"
     CDS             37279..38022
                     /gene="zapD"
                     /locus_tag="JN691_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAY7241579.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38032..38229
                     /gene="yacG"
                     /locus_tag="JN691_000034"
     CDS             38032..38229
                     /gene="yacG"
                     /locus_tag="JN691_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAY7241580.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38329..38718)
                     /gene="mutT"
                     /locus_tag="JN691_000035"
     CDS             complement(38329..38718)
                     /gene="mutT"
                     /locus_tag="JN691_000035"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAY7241581.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38778..41483)
                     /gene="secA"
                     /locus_tag="JN691_000036"
     CDS             complement(38778..41483)
                     /gene="secA"
                     /locus_tag="JN691_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAY7241582.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGKRKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41545..42057)
                     /gene="secM"
                     /locus_tag="JN691_000037"
     CDS             complement(41545..42057)
                     /gene="secM"
                     /locus_tag="JN691_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAY7241583.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42287..43204)
                     /gene="lpxC"
                     /locus_tag="JN691_000038"
     CDS             complement(42287..43204)
                     /gene="lpxC"
                     /locus_tag="JN691_000038"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAY7241584.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43305..44456)
                     /gene="ftsZ"
                     /locus_tag="JN691_000039"
     CDS             complement(43305..44456)
                     /gene="ftsZ"
                     /locus_tag="JN691_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAY7241585.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44517..45779)
                     /gene="ftsA"
                     /locus_tag="JN691_000040"
     CDS             complement(44517..45779)
                     /gene="ftsA"
                     /locus_tag="JN691_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAY7241586.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45776..46606)
                     /gene="ftsQ"
                     /locus_tag="JN691_000041"
     CDS             complement(45776..46606)
                     /gene="ftsQ"
                     /locus_tag="JN691_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAY7241587.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46608..47528)
                     /locus_tag="JN691_000042"
     CDS             complement(46608..47528)
                     /locus_tag="JN691_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase B"
                     /protein_id="HAY7241588.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47521..48996)
                     /gene="murC"
                     /locus_tag="JN691_000043"
     CDS             complement(47521..48996)
                     /gene="murC"
                     /locus_tag="JN691_000043"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAY7241589.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49050..50117)
                     /gene="murG"
                     /locus_tag="JN691_000044"
     CDS             complement(49050..50117)
                     /gene="murG"
                     /locus_tag="JN691_000044"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAY7241590.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50114..51358)
                     /gene="ftsW"
                     /locus_tag="JN691_000045"
     CDS             complement(50114..51358)
                     /gene="ftsW"
                     /locus_tag="JN691_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAY7241591.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51358..52674)
                     /gene="murD"
                     /locus_tag="JN691_000046"
     CDS             complement(51358..52674)
                     /gene="murD"
                     /locus_tag="JN691_000046"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAY7241592.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52677..53759)
                     /gene="mraY"
                     /locus_tag="JN691_000047"
     CDS             complement(52677..53759)
                     /gene="mraY"
                     /locus_tag="JN691_000047"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAY7241593.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53753..55111)
                     /gene="murF"
                     /locus_tag="JN691_000048"
     CDS             complement(53753..55111)
                     /gene="murF"
                     /locus_tag="JN691_000048"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAY7241594.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55108..56595)
                     /gene="murE"
                     /locus_tag="JN691_000049"
     CDS             complement(55108..56595)
                     /gene="murE"
                     /locus_tag="JN691_000049"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAY7241595.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56582..58348)
                     /locus_tag="JN691_000050"
     CDS             complement(56582..58348)
                     /locus_tag="JN691_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAY7241596.1"
                     /translation="MKAAVKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGTIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58364..58729)
                     /gene="ftsL"
                     /locus_tag="JN691_000051"
     CDS             complement(58364..58729)
                     /gene="ftsL"
                     /locus_tag="JN691_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAY7241597.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58726..59667)
                     /gene="rsmH"
                     /locus_tag="JN691_000052"
     CDS             complement(58726..59667)
                     /gene="rsmH"
                     /locus_tag="JN691_000052"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAY7241598.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59669..60127)
                     /gene="mraZ"
                     /locus_tag="JN691_000053"
     CDS             complement(59669..60127)
                     /gene="mraZ"
                     /locus_tag="JN691_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAY7241599.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(60729..61733)
                     /gene="cra"
                     /locus_tag="JN691_000054"
     CDS             complement(60729..61733)
                     /gene="cra"
                     /locus_tag="JN691_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAY7241600.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(61815..61901)
                     /locus_tag="JN691_000055"
     CDS             complement(61815..61901)
                     /locus_tag="JN691_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAY7241601.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(61913..62404)
                     /gene="ilvN"
                     /locus_tag="JN691_000056"
     CDS             complement(61913..62404)
                     /gene="ilvN"
                     /locus_tag="JN691_000056"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY7241602.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDQTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(62407..64131)
                     /gene="ilvI"
                     /locus_tag="JN691_000057"
     CDS             complement(62407..64131)
                     /gene="ilvI"
                     /locus_tag="JN691_000057"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAY7241603.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPFVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(64449..65393)
                     /gene="leuO"
                     /locus_tag="JN691_000058"
     CDS             complement(64449..65393)
                     /gene="leuO"
                     /locus_tag="JN691_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAY7241604.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPS
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            66053..66139
                     /gene="leuL"
                     /locus_tag="JN691_000059"
     CDS             66053..66139
                     /gene="leuL"
                     /locus_tag="JN691_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAY7241605.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            66232..67803
                     /gene="leuA"
                     /locus_tag="JN691_000060"
     CDS             66232..67803
                     /gene="leuA"
                     /locus_tag="JN691_000060"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAY7241606.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            67803..68894
                     /gene="leuB"
                     /locus_tag="JN691_000061"
     CDS             67803..68894
                     /gene="leuB"
                     /locus_tag="JN691_000061"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAY7241607.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            68897..70297
                     /gene="leuC"
                     /locus_tag="JN691_000062"
     CDS             68897..70297
                     /gene="leuC"
                     /locus_tag="JN691_000062"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAY7241608.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKIMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            70308..70913
                     /gene="leuD"
                     /locus_tag="JN691_000063"
     CDS             70308..70913
                     /gene="leuD"
                     /locus_tag="JN691_000063"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAY7241609.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(70962..72140)
                     /gene="setA"
                     /locus_tag="JN691_000064"
     CDS             complement(70962..72140)
                     /gene="setA"
                     /locus_tag="JN691_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HAY7241610.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(72242..72373)
                     /gene="sgrT"
                     /locus_tag="JN691_000065"
     CDS             complement(72242..72373)
                     /gene="sgrT"
                     /locus_tag="JN691_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAY7241611.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            72462..74117
                     /locus_tag="JN691_000066"
     CDS             72462..74117
                     /locus_tag="JN691_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAY7241612.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEMPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      74151..74250
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            74281..75264
                     /gene="thiB"
                     /locus_tag="JN691_000067"
     CDS             74281..75264
                     /gene="thiB"
                     /locus_tag="JN691_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAY7241613.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWLVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESNLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            75240..76850
                     /gene="thiP"
                     /locus_tag="JN691_000068"
     CDS             75240..76850
                     /gene="thiP"
                     /locus_tag="JN691_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAY7241614.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVAIAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            76834..77532
                     /gene="thiQ"
                     /locus_tag="JN691_000069"
     CDS             76834..77532
                     /gene="thiQ"
                     /locus_tag="JN691_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAY7241615.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(77646..78410)
                     /locus_tag="JN691_000070"
     CDS             complement(77646..78410)
                     /locus_tag="JN691_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7241616.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(78496..79374)
                     /gene="araC"
                     /locus_tag="JN691_000071"
     CDS             complement(78496..79374)
                     /gene="araC"
                     /locus_tag="JN691_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAY7241617.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            79713..81413
                     /gene="araB"
                     /locus_tag="JN691_000072"
     CDS             79713..81413
                     /gene="araB"
                     /locus_tag="JN691_000072"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HAY7241618.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            81424..82926
                     /gene="araA"
                     /locus_tag="JN691_000073"
     CDS             81424..82926
                     /gene="araA"
                     /locus_tag="JN691_000073"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAY7241619.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYNDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            83126..83821
                     /gene="araD"
                     /locus_tag="JN691_000074"
     CDS             83126..83821
                     /gene="araD"
                     /locus_tag="JN691_000074"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY7241620.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            83896..86247
                     /gene="polB"
                     /locus_tag="JN691_000075"
     CDS             83896..86247
                     /gene="polB"
                     /locus_tag="JN691_000075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAY7241621.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            86412..89318
                     /gene="rapA"
                     /locus_tag="JN691_000076"
     CDS             86412..89318
                     /gene="rapA"
                     /locus_tag="JN691_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAY7241622.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            89330..89989
                     /gene="rluA"
                     /locus_tag="JN691_000077"
     CDS             89330..89989
                     /gene="rluA"
                     /locus_tag="JN691_000077"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAY7241623.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(90106..90921)
                     /gene="djlA"
                     /locus_tag="JN691_000078"
     CDS             complement(90106..90921)
                     /gene="djlA"
                     /locus_tag="JN691_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAY7241624.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            91176..93530
                     /gene="lptD"
                     /locus_tag="JN691_000079"
     CDS             91176..93530
                     /gene="lptD"
                     /locus_tag="JN691_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAY7241625.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEALGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            93583..94869
                     /gene="surA"
                     /locus_tag="JN691_000080"
     CDS             93583..94869
                     /gene="surA"
                     /locus_tag="JN691_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAY7241626.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDTTLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRGRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQLNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            94869..95858
                     /gene="pdxA"
                     /locus_tag="JN691_000081"
     CDS             94869..95858
                     /gene="pdxA"
                     /locus_tag="JN691_000081"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAY7241627.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            95855..96676
                     /gene="rsmA"
                     /locus_tag="JN691_000082"
     CDS             95855..96676
                     /gene="rsmA"
                     /locus_tag="JN691_000082"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAY7241628.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDHFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            96679..97056
                     /gene="apaG"
                     /locus_tag="JN691_000083"
     CDS             96679..97056
                     /gene="apaG"
                     /locus_tag="JN691_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAY7241629.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVHGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            97063..97905
                     /gene="apaH"
                     /locus_tag="JN691_000084"
     CDS             97063..97905
                     /gene="apaH"
                     /locus_tag="JN691_000084"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAY7241630.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(97983..98462)
                     /gene="folA"
                     /locus_tag="JN691_000085"
     CDS             complement(97983..98462)
                     /gene="folA"
                     /locus_tag="JN691_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAY7241631.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(98653..100514)
                     /gene="kefC"
                     /locus_tag="JN691_000086"
                     /pseudo
     CDS             complement(98653..100514)
                     /gene="kefC"
                     /locus_tag="JN691_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
     gene            complement(100507..101037)
                     /gene="kefF"
                     /locus_tag="JN691_000087"
     CDS             complement(100507..101037)
                     /gene="kefF"
                     /locus_tag="JN691_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAY7241632.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(101085..102479)
                     /locus_tag="JN691_000088"
     CDS             complement(101085..102479)
                     /locus_tag="JN691_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7241633.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(102538..102825)
                     /gene="fixX"
                     /locus_tag="JN691_000089"
     CDS             complement(102538..102825)
                     /gene="fixX"
                     /locus_tag="JN691_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HAY7241634.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(102822..104108)
                     /locus_tag="JN691_000090"
     CDS             complement(102822..104108)
                     /locus_tag="JN691_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7241635.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEASAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTSENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(104159..105100)
                     /locus_tag="JN691_000091"
     CDS             complement(104159..105100)
                     /locus_tag="JN691_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY7241636.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAC"
     gene            complement(105115..105885)
                     /locus_tag="JN691_000092"
     CDS             complement(105115..105885)
                     /locus_tag="JN691_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HAY7241637.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            106300..107814
                     /gene="caiT"
                     /locus_tag="JN691_000093"
     CDS             106300..107814
                     /gene="caiT"
                     /locus_tag="JN691_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HAY7241638.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWMFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            107845..108987
                     /gene="caiA"
                     /locus_tag="JN691_000094"
     CDS             107845..108987
                     /gene="caiA"
                     /locus_tag="JN691_000094"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HAY7241639.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            109116..110333
                     /gene="caiB"
                     /locus_tag="JN691_000095"
                     /pseudo
     CDS             109116..110333
                     /gene="caiB"
                     /locus_tag="JN691_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
     gene            110407..111960
                     /gene="caiC"
                     /locus_tag="JN691_000096"
     CDS             110407..111960
                     /gene="caiC"
                     /locus_tag="JN691_000096"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HAY7241640.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDHPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            112069..112854
                     /gene="caiD"
                     /locus_tag="JN691_000097"
     CDS             112069..112854
                     /gene="caiD"
                     /locus_tag="JN691_000097"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HAY7241641.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            112860..113450
                     /gene="caiE"
                     /locus_tag="JN691_000098"
     CDS             112860..113450
                     /gene="caiE"
                     /locus_tag="JN691_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HAY7241642.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(113536..113931)
                     /gene="caiF"
                     /locus_tag="JN691_000099"
     CDS             complement(113536..113931)
                     /gene="caiF"
                     /locus_tag="JN691_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HAY7241643.1"
                     /translation="MCEGYVEKLLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(114193..117414)
                     /gene="carB"
                     /locus_tag="JN691_000100"
     CDS             complement(114193..117414)
                     /gene="carB"
                     /locus_tag="JN691_000100"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAY7241644.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(117432..118580)
                     /gene="carA"
                     /locus_tag="JN691_000101"
     CDS             complement(117432..118580)
                     /gene="carA"
                     /locus_tag="JN691_000101"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAY7241645.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFLGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(119036..119857)
                     /gene="dapB"
                     /locus_tag="JN691_000102"
     CDS             complement(119036..119857)
                     /gene="dapB"
                     /locus_tag="JN691_000102"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAY7241646.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(120024..120938)
                     /gene="rihC"
                     /locus_tag="JN691_000103"
     CDS             complement(120024..120938)
                     /gene="rihC"
                     /locus_tag="JN691_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAY7241647.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(121004..121954)
                     /gene="ispH"
                     /locus_tag="JN691_000104"
                     /gene_synonym="lytB"
     CDS             complement(121004..121954)
                     /gene="ispH"
                     /locus_tag="JN691_000104"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAY7241648.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(121956..122405)
                     /gene="fkpB"
                     /locus_tag="JN691_000105"
     CDS             complement(121956..122405)
                     /gene="fkpB"
                     /locus_tag="JN691_000105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7241649.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(122492..122986)
                     /gene="lspA"
                     /locus_tag="JN691_000106"
     CDS             complement(122492..122986)
                     /gene="lspA"
                     /locus_tag="JN691_000106"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAY7241650.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(122986..125802)
                     /gene="ileS"
                     /locus_tag="JN691_000107"
     CDS             complement(122986..125802)
                     /gene="ileS"
                     /locus_tag="JN691_000107"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAY7241651.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYVEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(125845..126786)
                     /gene="ribF"
                     /locus_tag="JN691_000108"
     CDS             complement(125845..126786)
                     /gene="ribF"
                     /locus_tag="JN691_000108"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAY7241652.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(126794..127012)
                     /locus_tag="JN691_000109"
     CDS             complement(126794..127012)
                     /locus_tag="JN691_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAY7241653.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            127115..127378
                     /gene="rpsT"
                     /locus_tag="JN691_000110"
     CDS             127115..127378
                     /gene="rpsT"
                     /locus_tag="JN691_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAY7241654.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        30783 a        35052 c        32183 g        29688 t
ORIGIN      
        1 aggttggtac tgaaaacggg caactccttg gtaatactct cacaggtaat gacgcagcaa
       61 aaggagtcgg tgtactcata gaaggtttag caactagtaa aaatcctcta atgacattga
      121 aacctaatga ttcaaattct gtttataaag attacgaccc gagaggcaaa gacgatacaa
      181 caggaggagt ttacccggat caagatacag gtataacata ccctctccat ttccaggcca
      241 cgctacaaca ggatggaact ataccaatag aagctggtga gtttaaagcc accagtactt
      301 tccaggtaac ctacccttaa taagtccacc gcaccgccat catctggcgg tgcggtaatt
      361 gataaatcat tgcccccacc aatgtgactg ataaaatgcc aggcaacacg acatcattta
      421 tcaggatacg ttatgaaacc gaccaccatt gcttcacttc agaaatataa gcaggataaa
      481 aagcgtttcg cgaccatcac cgcttacgac tacagcttcg cgaaactatt tgctgatgaa
      541 gggcttaacg tcatgctggt aggcgactcg ctgggcatga ccgttcaggg ccacgattcc
      601 actctgccag taaccgttgc tgatatcgcc taccacactg ccgccgtacg tcgcggcgca
      661 ccaaactgcc tgctattggc tgacctgccg tttatggcgt atgccacgcc ggaacaagcc
      721 tttgaaaacg ccgcaacggt tatgcgtgcc ggtgccaata tggtcaaaat tgagggcggt
      781 gagtggctgg tcgaaaccgt aaaaatgctg accgaacgtg ccgttcctgt atgtggtcac
      841 ttaggtttaa ccccacagtc agtgaatatt ttcggtggct acaaagtaca ggggcgcggc
      901 aatgaagcga gcgagcgact actcagcgat gcattagcct tagaagccgc gggggcacag
      961 ctgctggtgc tggaatgcgt gccggttgag ctggcaaaac gtattaccga agcactggcg
     1021 atcccggtta ttggtattgg tgcaggcaac gtcactgacg ggcaaatcct cgtgatgcac
     1081 gacgcctttg gtattaccgg cggtcacatt cctaaattcg ctaaaaattt cctcgccgaa
     1141 acgggcgaca tccgcgcggc tgtgcggcag tatatggctg aagtggagtc cggcgtttat
     1201 ccgggcgaag aacacagttt ccattaagga gtcacgttgt gttaattatc gaaaccctgc
     1261 cgctgctgcg tcagcaaatt cgccgcctgc gtatggaagg caagcgcgtg gcgctggtgc
     1321 ctactatggg taatctgcac gatggccata tgaagctggt cgacgaagcc aaagcccgcg
     1381 ccgatgtggt cgtcgtcagt attttcgtta acccgatgca gttcgaccgc ccggaagatc
     1441 tggcacgtta tccacggacc ttgcaggagg actgcgagaa gctaaacaaa cgtaaagtgg
     1501 atttagtttt cgccccttcg gtaaaagaga tctacccgaa cggtactgaa acccacactt
     1561 acgttgacgt tcctggcctt tcgaccatgc tggaaggtgc cagccgtccg ggacattttc
     1621 gcggcgtttc gactattgtc agcaagctgt tcaacctggt ccagccggac atcgcctgct
     1681 tcggtgaaaa agattttcag caactggcgt tgatccgcaa aatggttgcc gatatgggct
     1741 tcgatattga gattgtcggt gtgccaatta tgcgcgccaa agacggtctg gcgctaagtt
     1801 cccgtaacgg ttatctgacg gcggaacaac gcaaaattgc gcctggtctg tacaaagttt
     1861 taagttcgat tgctgacaaa ttgcaggctg gggaacggga tctcgatgaa attattacca
     1921 ttgcggggca agaactgaat gaaaaaggct tccgcgccga tgatattcag attcgcgatg
     1981 ccgacacatt gctggaagtt tctgaaacca gcaaacgggc agtaattctg gtagccgcct
     2041 ggcttggcga tgctcgcctg atcgacaaca aaatggtcga gctggcgtaa tacttaactg
     2101 gcgctacggc tgatggcgcc agttattaat ttaccccacg tcatctgtca taacacaggt
     2161 aacttaaata aggttaatta ccttatcaat ctcagcaaga ggtaaatttg ccatctctgc
     2221 aacgtcttcc cgagacattc ctttacttag aagacgcagg gcgaattctt gcctttcctc
     2281 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2341 catcatagac ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2401 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2461 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2521 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2581 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc
     2641 cggcgagtaa aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     2701 ataaaacagt atcggcacca ccagcggcag cttatcgtgg ccagcctcca gatgccggtg
     2761 catggcagca atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg
     2821 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     2881 gtccgtgctg tgacctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     2941 gttgagatca caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3001 cgtctccgca tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc
     3061 atccatcttc cctgtcttcc gtgtggcctt gcgtgaaggc agtatccagt caaggcgtgc
     3121 tatcgtcagc cacctcttta ttgtttaccg agcagcgttc ataaaaatat caattcgtta
     3181 caggcgttac atggcacgct tcggcgcggt acgcaaactg ctgacgcaaa tgccatcaga
     3241 tgagtagaca ctcatcaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac
     3301 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3361 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3421 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat cgatatctgg
     3481 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cagttcgaga
     3541 attatttctg ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc
     3601 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa tgtcgcctat
     3661 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     3721 ttgttatccc tgcggctggt tactcaccag ccgcgacatc ttctccagcg aatccgttct
     3781 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     3841 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     3901 gccgccaaac ggatacgaaa gataccagac atgtggatta aattgcgcca gagcgcggcg
     3961 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4021 ataaccatct acccgatgca aaaaatgggt atgtgactgg aaatcaaaaa catcgcgaat
     4081 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4141 acgtttgatg cgtgaggtaa caataaacac cgtcgccttc atgccatatt gtttcaacac
     4201 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4261 cgcagggaga ttgatcgtat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4321 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4381 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4441 cggcaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4501 acggatctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4561 caaattgtcc gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tgtagaccgg
     4621 cgtatctttc caggtaatta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     4681 gtcttcaact ttttgtcgcc cctgaactgg ctcgagatgc cctttatcga taaacccttt
     4741 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     4801 tccggcccga atattcccca cggtcaccat cttgtcacca atttgcgacc agaccgcagc
     4861 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     4921 catcagcagg agaataacag cttgtttata catgaaaaaa taaccgaggg tgaaacaacg
     4981 cttttattgt agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt
     5041 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5101 cagttcgaca atacgctcac gaaactctga actggtcatg gtttcacggc aagccatcat
     5161 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5221 taataatgaa gccacgcgat acggtggcgc tccggctata tcggttaaga agatgacacc
     5281 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5341 taatccgcgc cagaaattca cggcccggca ctgaagaagt gccccatgtt ttttctccag
     5401 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5461 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5521 caaaactcct ctcccccgaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5581 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     5641 ccaaaggtag tgaccagcgg cacatcattt ataccgagga agccgtagcg gaaaccactg
     5701 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     5761 agtgagtaaa agaccccgcc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     5821 ctgatgtcat cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag ggagaatagc
     5881 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     5941 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6001 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6061 ttacgctgga acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatc
     6121 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6181 ccaatacgcg aacctattag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6241 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6301 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6361 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6421 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgtagc acttcaactt
     6481 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6541 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6601 aggtattttc caccagctca ccatgttgaa taatgccgat attgcggcac agcatttctg
     6661 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     6721 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     6781 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     6841 cagataacat acgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt
     6901 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     6961 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7021 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7081 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7141 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7201 gaacgccgcc tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7261 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7321 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7381 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7441 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7501 cgttcctgca gaacgtttaa ccggccttga gccgggcgaa ctctttgttc accgtaatgt
     7561 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7621 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     7681 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     7741 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     7801 gtgtgaactg aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc
     7861 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     7921 cttgctgcgt gatctggatg ttaccgccac caaccgcgaa acccttgagc aacgttaccg
     7981 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8041 atgcaacaca tccggcaact tcacacttac tcgtccagca gaatcacttt gccaatatac
     8101 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8161 atcgagaaac cgataaattc aaccgggacg ttcacttcac gacgggacgg tttatccagc
     8221 agcgtacaaa tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8281 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatcc
     8341 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8401 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8461 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8521 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8581 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     8641 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     8701 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     8761 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     8821 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     8881 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     8941 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg
     9001 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9061 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9121 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9181 gtggtcgcac tgctgattag cggtggtatc ctgacctggg ccggatttaa cgatccgcag
     9241 gagatcaacg gcaccttaag cgccgatgcc acacctgccg aagctatctc ccccgttgcc
     9301 gatcaggact ggcctgcgta tggccgcaat caggaaggtc aacgcttttc accactgaag
     9361 caaattcacg ccgataacgt ccacaagctg aaagaagcct gggtgttccg tactggcgat
     9421 gtgaagcagc cgaacgatcc gggtgaaatc accaatgaag tgacgccgat taaagtgggc
     9481 gatacccttt acctgtgtac cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc
     9541 aaagagaaat ggcattacga tcctgagctg aaaaccaacg agtctttcca gcacgtaacc
     9601 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
     9661 gattgcccgc gtcgtatcat tcttccagtg aatgatggtc gcctgattgc gattaacgct
     9721 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
     9781 atgccagaca ccaaaccggg tctgtatgag ccgacttctc cgccaattat caccgataaa
     9841 accatcgtga tggccggttc agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg
     9901 atccgtggtt ttgatgtcaa caccggtgag ctgctgtggg cttttgatcc gggcgcgaaa
     9961 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcacc aaactcgtgg
    10021 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10081 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10141 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10201 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10261 gtgccgatta tttacgctcc ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat
    10321 ggcgaactgg tggttccggc cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat
    10381 tacgttaccc ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc
    10441 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10501 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggtacgct ggtcttcccg
    10561 ggtaacctgg ggatgttcga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10621 attgccaacc caatggcact gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac
    10681 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    10741 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt
    10801 aaacagccag catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg
    10861 aagaaacgta ttggtacgcc gcaggacagt atgccgttcc cgatgccggt tccggtgccg
    10921 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    10981 atcgccgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11041 tggcagggtc gtttaccggc gggtggtcag gcaacaccga tgacctatga agtgaatggc
    11101 aagcagtacg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11161 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11221 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11281 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11341 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11401 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcgtg ccgtgaatat
    11461 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11521 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11581 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    11641 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    11701 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    11761 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    11821 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg
    11881 ccatccccat ctggctgacc ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct
    11941 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12001 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12061 aattgagcga gcgggcatta cagccattga gcaaacgcag gcgcagccaa ccacgcgggg
    12121 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12181 cggcggtcat cacatccagt tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct
    12241 gaacgatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12301 cgtcatcttc aatcaccacc agaccagcca gccccatcgc cacctgtcgc ccggtcttgc
    12361 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacctcaccg
    12421 agcgcttgcc acctggcgga ataattccct gtgggccgcc gtcgacgtca cccggtactt
    12481 ccagcccgtg ccagtgtaac gtcgtctctt ccgccagttg gttgtagata tcaaccgtta
    12541 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12601 tagttgcagt tttctcgcca aaggtggact gacctgcgcc aatagttaac tgaatgcgat
    12661 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    12721 ctgcacggct ccacagtggc aaggctgaag ccacacccag tgcgacggaa tatttcaaga
    12781 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    12841 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    12901 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    12961 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgttt gcgcaaacag
    13021 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13081 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13141 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13201 tctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaaatg
    13261 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13321 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13381 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13441 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13501 gaccgatcac caggatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13561 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13621 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    13681 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    13741 cgatgcgggt gtcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    13801 cgacgatccg cgctttcaac tagtgatcga cgatggcgtc aatttcgtta atcaaaccag
    13861 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagcct
    13921 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    13981 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagcc atcgcaaact
    14041 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14101 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14161 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14221 tacagcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14281 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14341 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14401 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14461 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14521 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14581 agaacacccc ggcccactgc cagaaacggt ggttgctcat ctcgataaaa gtcatatttg
    14641 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    14701 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    14761 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttactc gcgacattaa
    14821 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    14881 tatgaaggcg ctgtatgaca tggtggatgt aaacgtctat caggaaaata tcttccatac
    14941 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15001 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15061 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15121 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15181 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccctc ttccatttca
    15241 tcgccagcga attccagttg attggcgcga accatcacct cttcagcgtc catccacagg
    15301 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15361 tgttccactt catcaagcaa ggccaggttt tctttcacct cttcattaaa ccagtgcccg
    15421 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15481 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca tcgcgccatt
    15541 gatgtaatta tcgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15601 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    15661 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    15721 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    15781 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    15841 gcagaagcca ggaagacatt cgcgccagta cccaaacggt tcgggaagtt acgggtagag
    15901 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagggaacag
    15961 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc
    16021 agttgcgcgg cgtccatacg ggttggcggt gccacccaca ggcgggtcgg caactgacct
    16081 ttgtgcgcat ccagcagttt accagcggca cggaagtgac cgatgttagt catgcaagaa
    16141 ccgataaata cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc
    16201 ggatcgttcg gagcacacag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16261 gccgcgtatt ccgcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16321 atgccctgaa tacggcgttc cagggtacga cgatcgccgt aaccttccgc gatcatccac
    16381 ttcagcagga cgatgttgga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16441 gtacaaccag cggcagaacg ctcggcggac gcatcggtta gctcaaaggc ctgctcaact
    16501 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16561 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcggaatggc gtgtaccaga
    16621 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    16681 atatccagcg gcattacgcc agttgcggca gcaaacgcca ccagaccaga acccgccggg
    16741 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    16801 agcagcatac ggttcagcca ggagtgaatg acgccgtcac ccggacgcag cgacacaccg
    16861 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    16921 tacgccgcgg tgtggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    16981 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc
    17041 attttcggtt cgcagtacgc gcccggacga atgcctttca cgccacaggc acggcctacc
    17101 attttctgcg ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17161 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17221 atcagcggaa tacggccgcc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17281 aaggtcgcca gcagttcgcc tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17341 tcaatcacgt cgcccatgtt caggttagag acgttgactt cgattggcag tgcacccgcg
    17401 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17461 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17521 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17581 tgttgcagag cttcaatctg tttgatcggg ccaacaacac ctggctggtc tggctcaata
    17641 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac
    17701 gcatccggtg ccggagaaag gtcatcggtg ttggtttcgc cagtgacttt aaagacggta
    17761 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    17821 tgcattacct gcttcgcata ttcgttgcct gctttcgctt tctcttctac gtcatagaag
    17881 ttatcgaaca tcagcagcgt gtgagaaagt gctttggcag caataggtgc cagtttggca
    17941 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt gcccagcagt
    18001 tcgatagctt tttccggagt cagcagaggg gatttagctt cgcctttagc gatagcagcc
    18061 aggaagcctg cttttacata ggcggcttca tcgacgcctg ggggaacacg gttggttaac
    18121 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg
    18181 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18241 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18301 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18361 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18421 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18481 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18541 cgcctcttta tactcggatt cacagcacct gcgggtggca gttcgccgac cattgcgatt
    18601 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcgctaa
    18661 tttgcagtgc cgggctttgt gctgcctctg ccgggctata tataaaaagc cgcacagtgg
    18721 aagcgcctgt agaaccgcaa tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc
    18781 ttcctgcaac ggtttccgca cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca
    18841 ctgcacaaat agatcaatgg gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc
    18901 aggtgctgat ggcatcaacc tatccgacaa acgttgctca agcagtgcaa tggtcgcacg
    18961 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19021 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19081 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19141 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19201 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcccccgtca
    19261 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19321 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19381 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19441 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19501 acgcactatt cagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19561 atgatgatta tcatcaccac gatggcggtc atcgtgacag taatggctgg caacacaacg
    19621 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    19681 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    19741 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    19801 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    19861 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcatcggca cagcggttta
    19921 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    19981 tgacccagca acaaaaggac gccgctcgtc agcgttatca gtcggcctct cctgagcagc
    20041 gccaggcagt ccgcgagaaa atgcagacta acccgcagat ccagcagcga agggaggcgg
    20101 cgcgccagcg tattcagtcc gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc
    20161 agactaaccc gcagaaccag cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa
    20221 ctcccgaaca gcgccaggtg tttaaggaaa aagtacagca gcgcccactg aaccaacagc
    20281 aacgtgataa cgcccgccag cgtatacaat cggcatcacc tgaacaacgt caggtttttc
    20341 gggagaaagt tcaggagagc cgcccacaac gtctaaacga cagtaaccat actgtcaggc
    20401 tgaataacga gcaacggtca gcagtacgcg aacgtctctc tgaacgtgga gcaagaagac
    20461 tggaaaggta aattgcagac gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg
    20521 gatgttccgg caaacgaaaa attacttctt cttcgctttc gggttcggca ggtcggtaat
    20581 gctaccttcg aacacttctg ccgccaggcc cacagactcg tgcagagtcg ggtgcgcgtg
    20641 gatggtcagt gcgatgtctt cagcatcaca acccatttcg attgccaggc cgatttcacc
    20701 cagcagctcg ccgccgttgg taccaacaat cgcaccaccg atcacacggt gagattcttt
    20761 gtcgaaaatc agcttggtca taccgtctgc gcagtcagaa gcgatagcac gaccagaagc
    20821 agcccacggg aaggtggcgg tttcatagct gatgcctttc tctttcgctt ctttctcagt
    20881 cagacctacc catgcaactt ctggttcggt ataggcgatg gacgggataa ctttcggatc
    20941 gaaatagtgt ttcttaccgg cgataacttc agcggcaacg tgaccttcgt gaacaccttt
    21001 gtgtgccagc atcggctgac cgacgatatc gccgatagca aagatgtgcg gtacgttggt
    21061 acgcagctgt ttgtcaacgc ggatgaaacc acggtcgtca acttccacgc cagctttgcc
    21121 tgcgtcgagg tttttaccgt tcggcacacg accaatcgct accagcacgg cgtcgtaacg
    21181 ctgcggttca gctggtgctt ttttgccttc catcgtcaca taaataccgt cttctttcgc
    21241 ttcaacggcg gtaactttgg tttccagcat caggttgaat ttcttgctga tacgcttggt
    21301 gaagacttta acgatgtctt tgtcagctgc cgggataacc tggtcgaaca tttcaaccac
    21361 gtcaatctgt gaacccagcg cgtggtaaac ggtgcccatt tccagaccga tgataccgcc
    21421 acccattacc agcaggcgtt ctggtacttc tttcagttcc agcgcgtcag tggagtccca
    21481 gatacgcgga tcttcatgcg gaataaacgg cagttggatt gggcgagaac ccgctgcaat
    21541 gatcgcattg tcgaagttga tcacggtttt gccgttctca ccttcaactt ccagggtgtt
    21601 agccccggtg aatttaccca gaccgttgac cactttgact ttgcggcctt tcgccatacc
    21661 agccagacca ccggtcagct gattgatcac tttctctttc caggtacgaa tcttgtcaat
    21721 atcggttttc ggttcgccga agacgatacc gtgttcagcc agcgctttgg cttcttcgat
    21781 aacttttgct acgtgcagca gtgctttaga agggatacag ccgacgttca ggcaaacacc
    21841 gccaagggtg ttgtaacgtt ctacgattac ggtttccaga cctaaatcag cgcaacggaa
    21901 ggcagcggag taacctgcgg ggcctgcccc aagtaccacg acctgagttt tgatttcagt
    21961 actcatcatg acctctatat atttatctcc ggcggtcata cccgtcgtct ttcaggccgc
    22021 agcggcgtta gcttcacgcc ctcaccccag ccacttactt atgtaagctc ctggggattc
    22081 tggcgcttgc taccttgctg cacccagaaa tccatagggt atgtgaagta ataccctaac
    22141 caccaccggg tcgttctatc cgtcggtatt ttacaaaatt gttaacaatt tttaaacaac
    22201 aaacggcaac cgatttgtct attcgctaat aacctcaata cattcatgag attaccagaa
    22261 aaaagccggc cgttgggccg gctcttttac ttacatcacc agacggcgaa tgtcagacag
    22321 cgtgttgtta atgatggtaa tgaaacgggc accatcagca ccgtcgatca cgcggtggtc
    22381 gaaggagaga gaaatcggca gcatcagacg cggcacgaac tctttaccat tccacaccgg
    22441 ctccatcgcg gacttggaaa cgccgaggat agccacttcc ggcgcgttca caatcggcgc
    22501 gaagtgggta gtacccaggc cgccgatgct ggagatggtg aagcaaccgc cctgcatttc
    22561 gcccgcagtc agcttaccgt cacgcgcttt cttagaaata gtcatcagct cgcgagacag
    22621 ctcgatgatg cctttcttgt tgacgtcttt gaataccgga acaaccagac cgttcggggt
    22681 atccaccgcc acaccgatgt tgatgtattt cttcagggtc agacgctgac cgtcttccga
    22741 cagcgaacta ttgaagcgag gcatctgctc aagagctgca gcaacggctt tcatgatgaa
    22801 gacaaccggg gtgatcttca catccagctt acgtttcgcc gcttcttcgt tctgctgttt
    22861 acggaacgct tccagctcgg tgatatcggt tttgtcgaag tgggtaacat gcgggatcat
    22921 cacccagtta cggctcaggt tagcaccaga aattttctgg atacggccca gttccacttc
    22981 ttcgatttcg ccaaacttgc tgaagtccac cttcggccac ggcagcatgc cagggatacc
    23041 accgccagtc gccgccggag ctgcttctgc acgtttgata gcttctttca cgtaagcctg
    23101 aacgtcttcg cgcaggatac gacctttacg gccagtgccc ttcactttcg ccaggttaac
    23161 gccaaactcg cgtgccagac ggcggatcag cggagtcgcg tgaacgtaag cgtcgttttc
    23221 agcaaattca gatttgcctt ccgctttcgc agccggagct gctgccggag cttcagcttt
    23281 tgctgccgga gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc
    23341 ttcaacttcg aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt
    23401 cagttccttc acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc
    23461 tacggtaatc agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt
    23521 cacttcaact tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg
    23581 tgcaggggtc gctgccggag ccgcttcctg tttagccgcc ggagctgccg cgcctgcttc
    23641 acccgcgact tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac
    23701 tttgatctct ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc
    23761 ttctacggtg atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc
    23821 ggtcacttca acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc
    23881 cgcgggtgct gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc
    23941 tgctgcaccg tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc
    24001 aacagagact ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc
    24061 tttgtcgcct tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac
    24121 caggatctcg gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc
    24181 cattattctt ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat
    24241 ttggcgattg cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc
    24301 agcgccgcaa ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg
    24361 ctgtcggaac gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa
    24421 gtacggacct gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg
    24481 ttcatcacct gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag
    24541 cgttcacaat cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca
    24601 gaacctacgc cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg
    24661 gaaccggagc ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag
    24721 atacctttac ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg
    24781 ttcagcgtag tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga
    24841 ccgtcatgca taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc
    24901 gggatagtca gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc
    24961 agggtggtac gaccggaagt accgccgatc aggaagccac gcgcttgctg gtcgccagcc
    25021 gcccagcaca gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg
    25081 atcatcggca gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg
    25141 cccagctcgt tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca
    25201 acctgctcgc ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga
    25261 cggaacagac cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga
    25321 tctttgatcg acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta
    25381 gagatctctt tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca
    25441 agcttctcgg tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga
    25501 gcgtgcagat aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg
    25561 atatctgcat cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg
    25621 ttcattttct taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa
    25681 cctttaatgg tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg
    25741 aatgcagcgt agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg
    25801 tcagtccagt ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga
    25861 acgtacgcac catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc
    25921 agctggatca gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac
    25981 atcactttga tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc
    26041 ttgccgttac cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc
    26101 aggttatcca gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc
    26161 atttcaccgt cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg
    26221 tgttccagat atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata
    26281 gatacggtcg ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg
    26341 ccattgccgt gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc
    26401 aggaaagcac gggcgtaaac gcccggggag atgtggccct ggaagtaaac caagtcgccg
    26461 ccatcctgct cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg
    26521 gaagactgga aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc
    26581 accgtcatga tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta
    26641 cccggatact ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg
    26701 cctgcggcta cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac
    26761 tgagcacgct caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga
    26821 gtttcgatcg gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc
    26881 taataacgtt gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag
    26941 acaggttcta cgtttagttg ccgcgctttt atatgcgctt gatttacaac atcttctgga
    27001 taatttttac cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc
    27061 acggcggctc tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg
    27121 atgcgatgct tcgcgcgctt cttccggctt accggccata atcgcttcaa atatgcgggt
    27181 gcggtgacta ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg
    27241 gcggacattc tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt
    27301 gtgggccgct tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag
    27361 atcgccagac tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc
    27421 ttcatcggta ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt
    27481 ttcgagcaag tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct
    27541 gaagctttgc cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa
    27601 gcccttcgct tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg
    27661 ttttgccagt tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat
    27721 caaaaactcc agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta
    27781 ggccatgagt tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt
    27841 ctgtgacgct aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca
    27901 tgaaactgtg cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca
    27961 caaatgtaga ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg
    28021 aattgaaccg attcacttac caattttttg atttttaagt caataaaaac gaatttaaat
    28081 tcactctgca tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa
    28141 ctcatcttca accatgcata aagcgggtgc attcgctgcc gtataccatt attcttgatc
    28201 tgacggaagt ctttttgtaa caattcaaac ttccttgatg taaataaatc aatacaacaa
    28261 acggaattgc aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga
    28321 ggtttcatga tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc
    28381 catattcagc ttatcgcgct gggtggcgcg atagggacag ggctattcct gggtagcgcc
    28441 tccgtaatac agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc
    28501 gcctttctga tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc
    28561 tttagccact ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac
    28621 tactgggtac tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt
    28681 cagttctggt atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt
    28741 aacgccatca acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt
    28801 atcaaagtta ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc
    28861 aacggcggcc cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac
    28921 ggcttcaccg gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa
    28981 ctggtgggga tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact
    29041 aaccaggtta tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca
    29101 ctgatgccgt ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag
    29161 ttaggcgata cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc
    29221 gtgtacaaca gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg
    29281 aacgcgccaa aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg
    29341 gtgtctgcgc tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca
    29401 tttggcctgt taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt
    29461 ctggcgcata tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct
    29521 gctctgcttt atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg
    29581 attatgctga tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc
    29641 gtgttaggta tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa
    29701 tctctctacg ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta
    29761 aatcgtcagt agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac
    29821 cgctgccttc ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc
    29881 cagacgcgtt aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc
    29941 cggtaacgct ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg
    30001 cacccaatcc agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt
    30061 ctggtatcgc gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac
    30121 gggtccccag gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt
    30181 aatccacagc aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg
    30241 gacgccgtga atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg
    30301 tgaagcagct gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag
    30361 caaaccaatc agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc
    30421 gcgtaacagt aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag
    30481 agaaaaatgt ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc
    30541 gcccaactta aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt
    30601 gtctccttgc tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt
    30661 ttccgatccg gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga
    30721 tctatcagtg cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga
    30781 gtatcggtgc cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga
    30841 tactgagaga ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca
    30901 ccatcacggc gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa
    30961 ggatgtgcct gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac
    31021 tcgcctggcg gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg
    31081 cggcaatcgt aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac
    31141 aacatgcagg atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac
    31201 cttatgattc gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca
    31261 cccgacgtga cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg
    31321 cgctgcggga agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt
    31381 taacggaaaa ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg
    31441 gcaaacgctg ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct
    31501 ggcatgtgga tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc
    31561 catcccgcgt gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag
    31621 gagttgccag taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt
    31681 tggatacgcg caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg
    31741 gcggcggagc gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata
    31801 ttattgcctc cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg
    31861 cgccagtaga agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag
    31921 ccgatatcat catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca
    31981 ccaacggcaa ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat
    32041 ttgccgaaac gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac
    32101 tcgacctttc aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg
    32161 ataatctccc cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc
    32221 aacgaaataa aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc
    32281 tgatggcgtt tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa
    32341 cgcggtttta cacttatcga actgatggtg gttattggca tcattgccat tttaagcgcc
    32401 attggtattc ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa
    32461 acctttgtgc cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat
    32521 acctgcgacg gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc
    32581 atgagtgtgg caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc
    32641 gtcgtcatga cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc
    32701 aatattcaaa gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac
    32761 gccaactaag gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag
    32821 ggagtcttgc tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca
    32881 catgagttgc tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg
    32941 acgcgccaac aaatggaagg tcacgccagt cgcacacaac agacattgcc cgtagctgtt
    33001 caggagaagc atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa
    33061 caacgcgcgt ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt
    33121 atcgacggcg tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca
    33181 gccagattaa aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg
    33241 caattcactg tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt
    33301 cggggtggtg aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt
    33361 aacacgctgg gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca
    33421 cagggactgg tgctggtaac cggccctaca ggcagcggca aaacggtcac gctttatagt
    33481 gccctgcaaa cgctgaatac cgctgacatt aatatttgta gcgtcgaaga tccggttgag
    33541 atccccatag ccggactaaa ccagacgcaa atccatccgc gtgccgggct cacctttcag
    33601 ggcgttttgc gtgcgttatt gcgccaggat cctgacgtca tcatgatcgg agagatccgc
    33661 gatggcgaaa cggcagaaat tgccattaaa gccgcgcaaa ccggtcacct ggtgttgtcc
    33721 acgctacaca ccaattccac cactgaaacg ctggtacgtt tacagcaaat gggggtcgcc
    33781 cgctggatgc tatcatcggc gcttacgctg gtaatagccc agcgtctggt acgtaaactt
    33841 tgcccacatt gtcgccgaca gcaaggggag cccatccata ttccagacac tgtatggccg
    33901 tcgccgctgc cccactggca ggcacccggt tgtgtacatt gctaccacgg tttttatggt
    33961 cgtaccgcct tgtttgaagt cctgcccatt acaccagcca tacgccagct tatttccgct
    34021 aatactgaag ttgaatcgct ggaaacgcac gcccgacagg cgggtatgcg tacgcttttt
    34081 gaaaacggct gcctggccgt ggagcaaggc ttaaccacct ttgaagagtt aatccgcgta
    34141 ctggggatgc cgcatggcaa gtaagcaact ctggcgctgg catggcataa ccggcgacgg
    34201 caatgcgcaa gatgggatgc tatgggcaga gagccgtact ttactgctta tggcactaca
    34261 gcaacagatg gttaccccac taagcctgaa gcgaatcgcc atcaattctg cgcagtggcg
    34321 aggagataaa agcgcggaag tcattcatca actggcgacg ctacttaaag ccgggttaac
    34381 gctttctgaa gggctggctc tgctggcgga acagcatccc agtaagcaat ggcaagcgtt
    34441 gctgcaatcg ctggcgcacg atctcgaaca gggcattgct ttttccaatg ccttattacc
    34501 ctggtcagag gcatttccgc cgctctatca ggcgatgatc cgcacgggtg aactgaccgg
    34561 taagctggat gaatgctgct ttgaactggc gcgtcagcaa aaatcccagc gtcagttgac
    34621 cgacaaagtg aaatcagcgt tgcattatcc catcatcatt ttagcgatgg caatcatggt
    34681 ggttgtggca atgctgcatt ttgttctgcc ggagtttgcc gctatctata agaccttcaa
    34741 caccccacta ccggcactaa cgcaggggat catgacgctg gcagacttta gtggcgaatg
    34801 gggctggctg ctggtgttgt tcggctttct gctggcgata gccaataagt tgctgatgcg
    34861 ccgcccgacc tggcttatcg cgcggcagaa attgctgtta cgcatcccga ttatgggttc
    34921 actgatgagg ggacaaaaac tcacgcagat ctttacgatt ctggcgctga cacaaagtgc
    34981 aggcattact tttttacagg gcgtagagag cgtcagagaa acaatgcgct gcccgtactg
    35041 ggtgcaactt ctgacacaaa tccagcacga tatcagtaac ggtcatccca tctggctggc
    35101 gctaaaaaat gccggtgagt ttagcccgct ctgtttgcaa ttagtgagaa caggagaggc
    35161 atccggctcg ctggacctca tgttagacaa cctcgcccat catcatcggg ataacacaat
    35221 ggcgctggcg gataacctcg cagccttact ggaaccggcg ttgctgatca taacgggagg
    35281 aattatcggt acgctggtgg tggcaatgta tctgccaatt ttccatttag gcgatgcgat
    35341 gagtgggatg ggataacgct ggcgtgttgc tccacgccag cgttgggaga ttacaggttg
    35401 ttgaagatgc ggttttcttg ttccagcaca cgaataaacg tggtgcgctt ggtcagctct
    35461 ttcaggcgtg aagccccaac gtatgtacaa gctgaacgca ggccgcccaa aatatcacgc
    35521 gcggtatttt caaccgggcc tcgcagcggc agcttaacgg ttttaccttc tgctgcgcgg
    35581 tattccgcaa cgccgccaac gtgacgtttc atcgcagact cggagctcat gccgtagaac
    35641 agcataaatt tctcgccgtt ctcctcaacg atgcgaccgc cgctctcttc gtggcccgcc
    35701 agcatgccgc caagcatgac gaaatcggca ccgccgccaa aggctttcgc cacatcgccc
    35761 ggcgtggtac agccaccgtc gctgacgatc attccgccca gaccgtgcgc cgcatcggca
    35821 cattcgatta ccgcagaaag ctgcggataa ccgacgcctg ttttgacgcg agttgtacaa
    35881 acagaacctg ggccaatgcc aactttaacg atatcagcac cggagaggat aagctcctca
    35941 cacatttcac cagtcactac gttgccagca caaatggttt tggtcggcca cgcttcacgc
    36001 gctttcgcaa cgaactgcac gaagtgttcg gaataaccat tcgccacatc aatacaaacg
    36061 aagtttaatg ccgggttcag gtcgagaatc tgtttagttt tttcgaaatc cgcatcagac
    36121 gtgccggtag aaaccatcac atgtttcagc acatcagcgg aagaattgtt gataaacgct
    36181 tgccactctt cgacagaaaa gtgtttatgc acagcagtca gaatatcaaa agaagccagc
    36241 gcagaggcca tagaaaatgt gcctacggtg tccatatttg cggcgataat cggcacgccg
    36301 gaccagctct gacctgaatg tttgaaggtg aattgacgtt ccagttcaac atcggaacgg
    36361 cttttaagag tggagcgttt agggcggatg agaacgtctt taaaacctaa cttcagatct
    36421 tcttcaatac gcatgtgcgg attcctgggg ttaatggcta taagggtaag agcgcaactc
    36481 cagtgacgct atcatacgca ctaatcaatg cggcgcaaga ctgcgaaatt gtcttttttt
    36541 acgctacaat cccataaatt tacctggtaa atagtgagcg aacgtttcat caatttcact
    36601 ttcgcgcgct catttttaac gttttgataa tcatgatgaa ttcccgggaa tataaattat
    36661 ggggtatata gttgccttaa cgggaggcat tggcagtggt aagagtaccg ttgccaatgc
    36721 gtttgctgat ctcggaatta acgtcattga tgccgatatt attgcgcgtc aggtggttga
    36781 accaggtgca cctgcgctac atgccattgc tgatcacttt ggcgaaaaca tgattgctgc
    36841 tgatggaaca ttgcagcgcc gggccttgcg cgagcggatc ttcgccaacc cggaagagaa
    36901 aaactggctt aacgacctgc tgcatccgct gattcagcaa gagacgcaac accagatcca
    36961 gcaagcaact tccccctatg tactgtgggt tgtgccattg ctggtagaaa actcactgta
    37021 taaaaaagcg aatcgagtgc tggtggtgga tgtcagccca gaaacgcaac ttaagcgcac
    37081 catgcagcgc gatgatgtaa ctcgcgaaca tgtcgaacaa atccttgctg ctcaggcaac
    37141 gcgcgaagcc cgccttgccg tggcagatga cgtcattgat aataacggcg caccggatgc
    37201 tatcgcatcg gatgttgccc gcctgcacgc acactatttg cagcttgcgt cgcagtttgt
    37261 ctcacaggaa aaaccgtaat gcagacccag gtcctttttg aacatccact aaatgaaaaa
    37321 atgcgtacat ggctgcgcat tgagtttttg attcagcaac tcaccgttaa tttacccatc
    37381 gttgaccacg ctggcgcgct gcatttcttc cgtaatgtca gtgaattact ggatgttttc
    37441 gagcgcggcg aagtccgcac tgagctgttg aaagaactta accggcagca acgtaaactc
    37501 cagacctgga ttggcgtgcc tggcgtggac cagagccgta ttgaagcatt aattcagcag
    37561 ttaaaagcgg cggggagcgt attaatttcc gcgccgcgta tcgggcaatt tctgcgtgaa
    37621 gatcgtttga ttgctctggt gcgtcagcga ctgagcatcc caggcggctg ttgcagcttt
    37681 gatttaccta cattgcacat ttggctgcat ctaccccagg cgcagcgcga cagccaggta
    37741 gaaacctgga ttgccagcct gaacccgctc acccaggcac ttaccatggt gctggattta
    37801 attcgccagt cggccccctt ccgtaaacaa accagcctga atggttttta tcaggataac
    37861 ggtggcgatg ccgacttgct gcgcctgaat ctgtcgctcg attcacagct ttatccgcaa
    37921 atttccggac ataagagccg ttttgccatt cgttttatgc cgctggacac tgaaaacgga
    37981 caggtaccgg aacgtctgga tttcgaactg gcctgttgct aaggagtaaa aatgtcagaa
    38041 actattacgg tgaattgccc aacctgcggg aaaacggtgg tgtggggtga aatcagcccg
    38101 tttcggccat tttgctccaa acgttgtcag ctgatcgacc tcggagaatg ggctgctgaa
    38161 gaaaaacgaa tccccagcag tggcgatctt tccgaaagcg atgactggag cgaagagcca
    38221 aagcagtgac atttgtctga tgcctcacgt aggcctgata agacgcggac agcgtcgcat
    38281 caggcatctt gtgcaaatgt cggatgcggc gtgaacgcct tatccgacct acagacgctt
    38341 aagcttcgca attaccggtt cattggctgg cggaaaatca tcggcattaa gaccgaccag
    38401 cgacatccac tcaccgggtt gcccttcttt accccacggc accccttccc aactttcgac
    38461 cagccagaac cacagagtta tatgcctgtc cgggaattca tattccagtt tttcaaatag
    38521 cgaaaaatgt tgtggggtaa tcccgacctc ttcctgaagt tcacgcacca ccgcctgttc
    38581 cggcgtttcg cccatttcaa ttttaccgcc gggaaactcc agtttattcg ccatgtgcgc
    38641 atctgctgcg cgacgtgtta taaagatttc attgttctcg ttgcgaataa tacctaccgc
    38701 aatttgcagc tttttcattg tcttaaacct ataaaaaagg cgcagaatcc tgcgcctttt
    38761 acttcaactg ttagctttta ttgcaggcga ccatggcact gcttgtattt tttaccagaa
    38821 ccgcacgggc aaggatcgtt acgtcctact ttgcgtttac cggtttgcgc cgccagtgca
    38881 gctgcggctg cagagtcgtc atcctgatgg ctaagctgct gcatttgtgc taaacgctcg
    38941 gcttccatac gacgctgttg ttccagctcc tcaacctctt caggcatacg tacctgaact
    39001 ttgctcagcg tactgataac ttcatatttc aacgactcca gcatcgctgc aaacatggag
    39061 aacgattcac gtttgtattc ctgcttcgga tctttctgtg catagccacg caggtggata
    39121 ccctgacgca gatagtccat cgctgccagg tgctctttcc acagggagtc aagcgtttgc
    39181 agcatgacgc ctttctcgaa gtgacgcatc atctcagcac caaccacttc ttctttacgc
    39241 tgatacactt cgatggactg cgccagaatg cgctcacgca gcgtctcttc atgcagttct
    39301 ggttctttat ccagccactc ggcaattggt aaatcgaggt cgaaatcgtt cttcagacgt
    39361 tcctgcagcc ccggaatatc ccacatttct tccagcgact gtggcggaat gtaggcatca
    39421 atggtggctt tgaacacatc ttcacgaatg ctgttgatgg tttcgctcac atcgctgaca
    39481 tccagcagtt cgttacgctg ggagtaaatg gcgcgacgct gatcgttagc cacgtcatca
    39541 tattccagca gttgcttacg aatgtcgaag ttacgacttt caactttacg ctgggcgttg
    39601 gcaatcgctt tagtcaccca cgggtgttca atggcttcgc ctggcttcat acccagttta
    39661 cgcatcatgc cggatactcg gtcggaagca aaaatacgca tcagcgcatc ttccatcgac
    39721 aggtagaaac gggaagaacc agcatccccc tgacgaccag aacgaccgcg caactggtta
    39781 tcgatacgac gggattcgtg acgctcggta ccgatgatat gcaggccacc tgcttccagt
    39841 accgcatcgt gacgtacctg ccagtcggct ttaatttttt caatttgctc tgcggtcgga
    39901 ttttccagcg cggcaacttc tgcctgccag ctacctccga gcacaatatc tgtaccacga
    39961 cccgccatgt tggtcgcgat agtcaccgca gccggataac ctgcctgagc aacaatcgcc
    40021 gcttcgttgg cgtggaattt ggcgttcagg acattgtgct taataccggc tttggtcagt
    40081 tcgtttgaca ccagctccga tttttcgatg gagatagtac ccaccagcac cggctggcct
    40141 ttcgcagtac gttctttgat atcttcaatg atcgcctgaa ttttttccgc ttcagtcatg
    40201 tagactaggt ccggcagatc tttacgaatc attggacggt tggtcggaac aacgacggta
    40261 tccagtttgt agatggagct aaattcgaaa gcttcggtat cagcagtacc ggtcatcccc
    40321 gccagttttt catacagacg gaagtagttc tggaaggtga tcgaagccag cgtttggttt
    40381 tcgttctgga tctgcacacc ttctttcgct tccacagcct ggtgcagacc atcggaccag
    40441 cgtcggccct gcatggtacg accggtgtgt tcgtcaacga tgataacttc accatcttta
    40501 acgatgtagt cgacgtcacg ggtaaacagc gcatgagcgc gcagcgccgc cgttacgtgg
    40561 tgcatcagca tgatgttggc cggagagtac agagactccc cttcatccat gatgccctct
    40621 ttcaccagca gttcttcaat cagcaccaga ccacgttcgg tcaggttcac ctggcgagat
    40681 ttttcgtcca ccgagaagtg gccttcgccc tggaaggttt cggagtcttc tttttcctga
    40741 cggatcaggt gcggaataat tttattcacg cgtttataca tttccgagct gtcttctgcc
    40801 gggccggaaa tgatcagcgg tgtacgcgct tcatcgatca ggatggagtc cacttcgtcc
    40861 accagcgcat agtgcagttt acgctgtaca cgttcttcag ggctgaacgc catgttgtcg
    40921 cgcaggtagt caaagccgta ttcgttgttc gtaccgtaag tgatgtcagc tgcgtaagct
    40981 tcgcgctttg ccggtgctgg catgcccggc aggttgatac caacagtcag gccaaggaat
    41041 tcaaacagcg gacggttgtt ttcggcgtca cgttgcgcca ggtagtcgtt gacggtaact
    41101 acgtgcacgc ctttaccggt tagtgcgttc aggtaagcag gcagcgttgc ggtcagggtt
    41161 ttaccttcac cggtacgcat ttcggcgatg cagcgttcgt taagaaccat accgccgagt
    41221 aactgaacgt cgaagtgacg cataccaaag acgcgcttac ttgcctcacg caccacggcg
    41281 aaagcttccg ggatcagatt ttccagcact tcgccttttt ccagacgcgc acgaaactct
    41341 gcggttttcc ctttcagttc ttcatcggag agtttttcca tctccggttc catggcattg
    41401 atgatgttga ccactttgcg catccggcgc agggtgcgat cgttacgact accgaaaact
    41461 ttagttaaca atttgattag cataataaaa tctcaaacgc cccgcgttgc ggagttaata
    41521 aaatgaagta aaggtttatt gttgttaggt gaggcgttga gggccagcac ggatgccttg
    41581 cgcctggctt atccagacgg gcgtgctgaa ttttgcttgt ggggtaaaat gcgcataatc
    41641 aatgcgataa cccttttcag acggcgtgcc ttcctgggtc agcagcgcgc tgagcgtatc
    41701 cagtaatgca agatgttgcg cctgaagagg caaagattct tcagcaacgg gcagtgtttg
    41761 cggtgccatt gcgaaagaaa gatgacggat taccgtgcga atagcatgtt gatgccagta
    41821 atcaacggaa tagttcgaat tcggccggcg cgtgttcgct tccagcaagg ccaattgacc
    41881 aaagttaact ttggctgaag gctcgtggtt gcgggttgtc gcttttgcgg gcgcgtttgg
    41941 ttcggcggcg ttgctgagcg caggcaaacc taaactcgcc gcgaccatcc ctaataagag
    42001 atgcggccag aagtagcgtt taccaaactg tcgccagcgc gtcagtattc cactcacgtt
    42061 attgccatcc cgatcgaatt caaacgtatt tagcgcacaa atcttatcat taaagcctga
    42121 agtccacagc acgaatgaag ataagttgtg cgtaaaagtg cttaagaaaa cagcgttcgc
    42181 accgttttta tagctcttat aaaattcaaa aaaaaacgac tggttcacct agccggagag
    42241 agtgccagat ttgccagtcg aattttatac gacagtataa atgtcgttat gccagtacag
    42301 ctgaaggcgc tttgaaggcc aacggcagtt ctgcgtcgtc ctggaaggtc acatattccc
    42361 aggcttcctg tttcgccagg acagcctgca gcagtttgtt attcagtgca tgaccggatt
    42421 tataagcggt aaatgcacca ataatattgt gaccacacat gaacaagtca ccgatcgcat
    42481 cgagcatttt gtgacgcaca aattcgtctt caaaacgcag gccgtcttcg ttcagtacgc
    42541 gataatcgtc aacaacgatg gcacaatcga agctgccgcc caggcacaaa ccacgggact
    42601 gcagatattc gatatcacgc atgaaaccga acgtacgcgc acggctgatc tggcgcataa
    42661 acgcatcagc ggagaagttc atcgcatagc gctggttgct ggaatcaata gccggatggt
    42721 taaaatcgat ggtgaaatcc agcgaaaaac cattgtacgg cttaaattca gcccacttat
    42781 cgccatcttc gacacgaaca gtctctttga tgcgaacaaa ttttttggcg cagttcaact
    42841 cgtcgatacc ggcgtcaagc agcaggtata caaacggagc ggcgctgccg tccatgatcg
    42901 ggatttccgg cgcgttaact tcgataacaa tgttatcgat gcccaggccc gcgagagcag
    42961 cattgaggtg ctctacggtt gaaatccgta catcatgctc gttgaccaga cacgtacaga
    43021 gcatggtatc acgcacagat ttggcatcgg ccgggaaatc taccggtgga ttcaagtcgg
    43081 tgcgacgata gatgaccccg gtgttggccg gcgcagggcg taacgtcagg gtgactttct
    43141 tgccggtatg taaaccgaca cccgtcgcct gaacgatacg tttaagtgtc ctttgtttga
    43201 tcatcgtatt atctcgccaa attacctatc caaccgaagt gtactataca ttcggcgggc
    43261 cagtttagca caaagagcct cgaaacccaa attccagtca attcttaatc agcttgctta
    43321 cgtaggaatg ctgggatatc cagataatcc ggctctttcg cagtttgcgg cgcattgtca
    43381 ttcactactt tagcaaccgg cttctgctcc tgggtcagcg gagccatccc atgctgctgg
    43441 tagcgatcca tcactggctg ctgaacctgc ttattggtca ccagagtgat ttcaggacgt
    43501 ttgtccatgc cgatacctgt cgcaacaacg gttacgcgca gctcgtcatt catatccggg
    43561 tcaagagaag taccgataac cacagtcgcg ttgtcggaag caaatgcacg gatggtgtta
    43621 cctaccgttt cgaactcatc cagacgcagg tcgaagcccg ccgtgatgtt aaccagcacg
    43681 ccgcgcgcgc cagacaggtc gatatcttcc agcagcggag aagagatagc catttcagca
    43741 gcttcttccg cacggtcttc accgctcgcc acgccagaac ccatcattgc gtagcccatt
    43801 tcagacatta cggtgcgtac gtctgcaaag tccacgttca tcaaacccgg acgagtaatc
    43861 agttcggcga taccttgcac agcgcctttc agtacatcgt tcgctgcgcc aaacgcatcc
    43921 agcagggaga taccgcggcc cagaactttc agcagtttgt cgttcgggat agtgatcaga
    43981 gagtccacat gcttggacag ttcagtgatc ccctgctcgg cgaatgccat acgcttcttg
    44041 ccttcaaagt tgaaaggctt agtgacgaca gcaacggtca ggatacccaa atcttttgcc
    44101 acttcagcga cgactggtgc tgcacctgta ccggtaccac cacccatacc cgcagcaata
    44161 aagaccatgt ctgcaccttc cagcgccgca cgcaatgcat cgcgatcctc atcagccgca
    44221 ttgcggccaa cttctggatt agcgccagcg cccagtcctt tggtgatacc gctaccgatt
    44281 tgaatcgtct gtccaaccgc tgttttacgc agcgcttgtg catcggtatt taccgcgaag
    44341 aattcaacac cttcaatacg ctcgcgcacc atgtgttcaa cagcattacc gccgccgccg
    44401 ccgacgccga tgactttaat caccgcgtca ttggtaagtt ccattggttc aaacatagtt
    44461 tctctccgat ttgtgcctgt cgcctgaggc cgtaatcatc gtcggcctca taaaaattaa
    44521 aactcttttc gcagccaact attgagtcgc ttgatccacg agccaactga tgctgtaaca
    44581 cgtttttcta cttcagcttc accgttaaga tgtgactctt tcccatagtg aagcaatccc
    44641 accgccgtcg aataatacgg ctcctgagca taatccgtta aaccggtaat gttcagcggc
    44701 gcgccgatac gcacttgcgt atgaaacacg cgctgagcac aggctgcaag accttcgatc
    44761 tgcgctgcgc caccggttaa tacaatgcct gccgccaggt gatgtttaac cccttgttgg
    44821 cgaagctttt cctgcaactg caatatctct tcgttgacca ggttgagcaa ctcggtatag
    44881 cgcggctcga tcacctctgc cagggtctga cgttgcagac tccgcggcgg acgaccgcct
    44941 acgctcggca cttccacgct ctcatctttt ccaacgatgg aacccagcgc acaaccgtgg
    45001 cgaactttaa tcgcttcggc gtcgcttggc ggcgtgccaa aggcgtaagc gatatcactg
    45061 gtcacgacat tgcctgcata aggaattacc ttagtgtggc gcaatgcccc accggtataa
    45121 acggcgatat ccattgtacc accaccgata tcgacgacgc agacacccag ttcacgttca
    45181 tcttccgtca ataccgaata acttgatgcc agtccggcaa atatcagttg gtcaactttc
    45241 agcccacaac gttcaaccgc tttgacgatg tttttcgcca tatcgttgtg acatgtgatc
    45301 aggtgcactt ttgcctgcat ccgcacaccc gaaagtccta ccggattctt gatcccttcc
    45361 tgatagtcaa tcgcatactc ttgcgggatc acatgcagca cacgatgctc atcgcgcaca
    45421 cgcaccgatt tcgcggtatg gacgacattt tccacatctt cttgcgtcac ttcttcttca
    45481 gaaataggca ccataccaat ttcattctgg cagctgatgt gcttaccaga aagcgccaga
    45541 tataccgaag agatctgaca atctgccatc aattctgcct ggtcaatggc gcgttgtacg
    45601 cacttgacca cggattcgag gtcgttcacc ccgcctttat ccataccacg tgacgggcag
    45661 ctgcccacgc caatgatatt gaccataccg tcgggcagaa cttcccctac taaagcggca
    45721 accttcgcgg taccaatttc cagtcctact accagttttc tgtccgtcgc cttgatcatt
    45781 gttgttctgc ctgtgcctga ttttgttgct gagtagattc ctctggcggc aagggcgccc
    45841 agcctactgc cgctccagag tcataacgca aatcaacgta gctaatccgt ttgccatcgg
    45901 tttgcgcctg ctgctgtaaa accggataaa gttctacaaa gcgagccaaa cgtttcatcg
    45961 tatcgccccg gccaagattg agcttaatat cgttattcag cgtcaactgc caggaacgcc
    46021 gcgcggtcat cgccgcttcc ttcagagtaa atctgtcctt tgccagcatc tgccccattt
    46081 cgcgatagcc ctgcaacact tcattagcgc tgccttccgg gccatacagc attggaagca
    46141 cctgcttgct ggtgcgatct ggcggcacgc tgaaggtgtt tccttccgcg tctaccatgt
    46201 gttgatcatt ccaccgcgca atcggcacat attcaaccag atgaatcttc aattcatcag
    46261 gccactgctt tctgacgctc acctgcttaa tccacggcag gcgttgttct atttgcgtct
    46321 ggatgatgtt gacatcctgg gtcataaagg tacccggctc acccaatgcc aggatcgact
    46381 gccggatatc gtcattacgc gtgtaatggc gttcaccggt caacaccagc tttgagagcg
    46441 gcaggcgttg cgcatcttcc atccagctca acacgaccca gccgctcacc aacactgtcg
    46501 ttaaaacggt cagcaggaaa aggatccccg ccagacgcgt tccattattg cggcgagaag
    46561 aaacctcttc ttcgctgttt cgcgtgttca gagcagcctg cgacatatta gtccgccagt
    46621 tccagaattc gtactaccaa ctgcgagaag ctcatacctg cctgacgtgc cgccatcggc
    46681 accaggctgt ggctggtcat acccggtgag gtattggctt ccagcagata aaactgtcca
    46741 tcgctgtcca gcataacgtc aatacgtccc catcctttgc aacctaacgt cgtccatgct
    46801 ttcagcacta atgcctgcaa attggcctct tgtgacgctt ccagacctgc ggggcagaaa
    46861 tactgtgtct catcagagag atacttcgcc tcataatcat agaaggttcc ggacggttga
    46921 atacgtattg acggtaaaat ttcttcaccg agtatcgcaa ccgtgaactc cggcccactt
    46981 agccattttt caatcaatac ttcttcatcg tgctgaaatg ccaatcttaa tgcatcttgt
    47041 agagcatttt ctgctactac ttttgacatt cccacactgg aaccttcgcg gctcggctta
    47101 acgataaccg gcaaacccag agcagaaatt tctgctaact gcttatcgct caggcctttt
    47161 tcaaactctg cgcgggttaa tgctacccac ggcgcgaccg gtaaaccggc accttgccac
    47221 agaagtttgc tgcgtagttt atctattgaa agcgcagatg ccatcactcc gctgccggta
    47281 taaggcaagc ccatcagctc gagcatgccc tgcagcgtac catcttcacc gccgcgaccg
    47341 tgtagcgcga taaacacttt ctgaaaaccc atcgacttca gttgcgtcac gtcgacttct
    47401 ttcgggtcga caggatacgc atcaataccg ccttcacgca gcccggctaa caccgctgcg
    47461 ccagaattca gagaaacttc ccgctcagcg gaagtcccgc ccaacaggac cgcgatttta
    47521 tcagtcatgt tgttcttcct ccggagtttg cggcttcagt ttgatttcag ctaaagaacg
    47581 ggcaattttt ccgatattac cagccccctg aacgagaatc aggtcgttac cggttaatac
    47641 cggtgccagc atctcggcta cctgcgcagg gtccggcacc agaatgggat caattttccc
    47701 acgtccacga attgtgcgac acagcgaacg gctgtccgct cccggaattg gcgcttcgcc
    47761 agccggatac acttccagca tcaacagggt atcaacctgc gtcagcacat tggcgaaatc
    47821 atcatacagg tcgcgcgtac gggtaaaacg gtgcggctga aacagcatta ccaggttttt
    47881 atccggccag cctgcgcgcg ccgctttaat ggtggcgtca acttccgtcg ggtggtggcc
    47941 gtagtcatcg accagcattg ccgtaccgct tttaccattc actggctcca gcgggaattc
    48001 accgaggaaa tcaaaacggc gaccagtccc ctggaagctc tccagcgcac gcaaaatagc
    48061 ctcgtcgtca attccctctt ccgtagcaac cgcaaccgca gctgcggcgt tcagcgcgtt
    48121 atgacggcct ggcgcattca gggtgacgcg catcggctct ttgtcctgac gcagcagcgt
    48181 aaagtgcccc tgcgggccaa tctgctgata atcttctaca cgcacgtcgg catcttcgct
    48241 gaagccgtaa gttgtggtct gacgtcccac acgcggtaac aattcgcgga tcaccggatc
    48301 atcaacacac atcaccgcac gaccgtaaaa aggcaggttg tgcagaaaat taataaaggt
    48361 ctgttttaaa ttctcaaagt cgccctggta ggtatccatg tggtcggctt cgatattggt
    48421 gacaatcgcc accatcggtt gcagatgcag gaacgatgca tcactctcat ctgcttcggc
    48481 aatcaggtac cgaccatgcc ccaaacgcgc atgaaccccc gccgctttta ccagcccgcc
    48541 gttaacgaag gttgggtcga gccccgcttc tgcgtagatg ctggaaacca tcgcggtagt
    48601 tgtcgttttg ccgtgcgttc ctgcaatggc gatgccatga cgaaaacgca ttaactcagc
    48661 cagcatttcg gcacgacgga tcaccggaat acgcgcttca tgagctgcga caatttccgg
    48721 gttgtcggca gaaatggcgc tggaaacaac gaccacgctg gcatcacgta cgttttccgg
    48781 gcgatggttg aaataaatcg tcgcacccag attcagtaac tgctgcgtga ccggatttgg
    48841 cgctaaatcg gaaccactga tctgataacc ttcattggcc agaacttcgg caataccgcc
    48901 cataccggca ccaccaatgc cgacaaaatg tatgtgccga acgcgacgca tttcgggcac
    48961 gatggaacgc agttttgcca attgttgtgt attcattctt tacgccatta acttcttaaa
    49021 ttcatacgat gcaaaaggca tcgctacaat tacgcccggg cagcccggct cacttcattt
    49081 gccactcgct cggtggcatc cggaatggat gcagcgcggg cgcgttctgc catggttaat
    49141 aaggtttctc gcgaccaccc ggccagggtg ttggcgacag catccacgct aagctgtggc
    49201 tgctcgataa ttttggctgc gcccgctttt tccagcggta gcgcattcca gtattgctgg
    49261 cggtctttat gttgaaacgg cacaaacaac gccggtagtc ctgccgcggc gatttcactc
    49321 acagttaaag caccggagcg gcaaacgacg acatccgccc acgcatacgc cgccgccata
    49381 tcatcaataa attcagtcac tttatgctgc ggttgccccg cttcggcata cgcctgttca
    49441 acggattgtt gcgaaccttt gccgctctga tgccagatag tgactgaatc acccagtttc
    49501 gcagcaacct gcggcattgt ctggttaaga atgcgtgcgc cctgggaacc acccactacc
    49561 aacacacgaa ccggaccttc acgtccagcc aaacgttgct gcggcaacgg cagcgccagc
    49621 acatcggtac gcaccgggtt acccaccact tccgcattag ggaaagcgcc tggaaacgcc
    49681 tgcatcactt tggtagcaat cttcgccagc catttattgg ttaagcccgc aataccgttt
    49741 tgttcatgaa gtacaaccgg aatgcctaac gaccacgcgg ccagaccacc tggacctgat
    49801 acatagccgc ccataccgag taccacgtca ggtttgtacg ctttcataat cgcccgcgcc
    49861 tgacgccagg cgttgaagat acgcagcggc gcagctatca gtgcttttat accttttcca
    49921 cgcaaaccag agatacgaat gaaatcaatt tcgatgccat gttttggcac taagtccgct
    49981 tccatacggt cggcagtccc cagccagcga acttgccaac cctgagccat tagatagtgc
    50041 gcaaccgcca gtcccgggaa tacatgtcca ccggttccgc ctgccatcac cattaatcgc
    50101 tttccttgac cactcatcgt gaacctcgta caaacgcctg cgctttctcc agacgcgttt
    50161 cataatcaat acgcaacagc atcatgatgg ctgtcgacat aatcagtaag ctcgaaccac
    50221 cgtaactgat tagcggcaat gtcagacctt tggtcggtaa catccccgcc gccgcgccta
    50281 cgttaaccag cgcctggaag ctaaaccaga tgccaataga acaggcgaga aaaccggaaa
    50341 aacggtggtc aatttctaat gctttgcggc caatcgacat cgcacgaaaa gcgacgaaga
    50401 ataccattaa aagtgctagt accacaccga cataacctaa ttcttcgccg ataatggcga
    50461 aaataaagtc agtgtgcgct tccggcagat actccagttt ttgtaccgaa ttacctaaac
    50521 cttgccccca aagttcgccg cgaccaaacg ccatcagcga ttgcgttaac tgatagccgc
    50581 tgccaaaggg atcttcccac gggttccaga atgcggtaac acggcggata cggtacggtt
    50641 cggcgaggat cagcaacaca accgctgaaa tgcccatacc gataatggca atgaactgcc
    50701 acaatttcgc tcccgccagg aacaacatcg ccagcgtagt cacaaacaac accaccaccg
    50761 taccaaggtc tggctgtgcc agcagtaaca ctgccaacac cagaatcacg cccatcggtt
    50821 tcaggaagcc gcgcaggtta ttacgtactt cgtcgccttt acgcaccaga tagttggcga
    50881 tatagcaaaa cagcgacagt tttgtcagct ccgcaggctg gatacgcagc aaaccgagat
    50941 cgatccaacg cgatgcccct ttaacagcgc tacccactac cagcacgatc atcagcagga
    51001 tgatagatcc gagcagcatc gtggcactgt agcgttgcca gaactccatc ggcagacgca
    51061 gcgtaatgat cgccagaata aacgccaaaa tcagatagac accatcacgc ttcgcgaaga
    51121 agaacggatc gttggttaag cgttgcccta tgggcattga cgccgaggtc accatgataa
    51181 agccaatcgc cgcgaggccg aaggtcagcc acagtaaggt gcgatcgtac atgatcaggc
    51241 tgtcggtatc tttttcccgc gagcccatca cccagccctt tagcgccgtg gagatccaga
    51301 ccaggatact gaatcctggc aggcgcggca ttttcaggcg agggagagat aaacgcatca
    51361 acctaactcc ttcgccagac gggcaaactc attgcctcgt tgttcaaagt tcttgaactg
    51421 atcaaggctg gcacaggccg gggagagcag aaccatatcg cccggctgaa cacgcggagc
    51481 cagcaagcgc atagcctgtt ccatagtttc ggtttgttct gccacttccg ggcgtagcgc
    51541 cgccagctgc gcgccgtcac gaccgaaaca atacagacgt acgttatcgc cattcaggta
    51601 acgcgccagt gggctaaagt ccgccgattt accatcgccg cccagcaaca aatgcagcgt
    51661 gccgtctacg tgcaggccat tcagcgccgc ttccgtactg ccgacgttgg tcgcttttga
    51721 atcgttaatc cagcgcacac cgttatgctc cagcaccact tcaaagcgat gcggcagacc
    51781 ggtgaaggtg gttaacgctt tcaggctgct ggcgcgcggt aaccctgcgg catctgccag
    51841 cgccagcgcc gccagcgcat tggtgtagtt atgctgcccg gaaagtttca tctctttcac
    51901 attcagcact ttctcgcctt taacccgcag ccaggtttcg ccctgttgat gattcaggtg
    51961 atagtcaccc atgttgacgc caaagctgac gcagcgttca tccgcaccgc gaatcggcat
    52021 tgttaaggca tcatcagcat taaccacgca aactttcgcg ttttcgtaaa tgcgcagttt
    52081 tgctgcacga tactgttgta aaccaaacgg gtagcgatcc atatgatctt cagtcacgtt
    52141 cagaatggtc gctgctaccg cctgtaagct ggaggtggtt tccagctgga agctcgacag
    52201 ttccagcacg tacagttcac actcatcatc cagtagcatc aacgcaggca ggccaatatt
    52261 gccacccaca ccaacgttaa cccccgccgc tttcgccatt tcacccacta gcgtggtgac
    52321 cgtgcttttg ccattagaac cggtaatcgc cacaatcggt gcttgtgctt cgcgacaaaa
    52381 cagctcgata tcgccaacga tttcgattcc ggcatcagcg gcagcgctta aggatggatg
    52441 cgccagtgca ataccgggac tggcgacaat cagatctgcc gccatcagcc attcatcatt
    52501 cagactgccc gtgtggcgtt ctacggcttc gggtaattta tccaggccag gcggtgtcat
    52561 acgcgtatcc ataacgcgcg gcgtcacacc gcgagcgagg aaaaagtcca cgcaggaaag
    52621 cccggtgagg cccaggccga taatgacaac atttttaccc tggtaatcag ccatgattaa
    52681 cgtaccttca gcgttgccag accaatcagg accagcatca gcgaaataat ccagaaacgc
    52741 acaatgacgc gcggttccgg ccagcctttc agttcatagt ggtgatgaat cggtgccatg
    52801 cggaaaatac gttgtccgcg cagtttaaag gagccgacct gcaggatgac agaaagcgtt
    52861 tctaccacga acacgccccc cataatcacc agcaggaatt cctgacgtag cagtacggcg
    52921 ataatgccta acgcaccacc taacgccagc gaaccgacat cgcccataaa gacctgcgcc
    52981 ggataggtgt taaaccacag gaagcccagt cctgccccga ctatcgcggt acagacaata
    53041 accagttccc cggcgtgtcg cagatacggt atatgtaaat agctggcaaa gttcatgttg
    53101 ccggtcgccc acgccaccag cgcaaaacca ccggcgacaa atacggtcgg cataattgcc
    53161 agaccgtcaa gaccatcggt caggtttacc gcgttgccag tacccacaat gacgaagtaa
    53221 gccagcagaa tgtagaacag ccccagttgc ggcatcacat ctttaaagaa tgggaccacc
    53281 agctgcgttg cgggcgtgtc tttgccggca aggtacaaag cgaaagcaac acccagcgca
    53341 atgaccgaca tccagaaata tttccaacga gcgatcaacc ctttggtgtc tttacgcacc
    53401 actttgcgat aatcatcaac aaagccaata acaccgtaac ctaccagcac caccaacacg
    53461 caccagacgt acggattgga cgggtaagcc cacagcagca cggagatcac aatcgctgtc
    53521 aggatcataa tcccgcccat ggtcggcgta ccgcgcttgc tgaagtgtga ttcaggaccg
    53581 tcgttacgca ctacctgacc aaaggaaagt ttttgcaaat gagcaatcat gcgcgggccc
    53641 atccacaatg agatgaacag cgcggtcagc aggctgacga tggcgcgaaa cgtcagatag
    53701 gaaaagacgt taaagccgga ataatatttg accaaatgtt cggccagcca aactaacatg
    53761 tcccattctc ctgtaaagcg cgtactacct cttccatggc agcactacgt gaacccttaa
    53821 ctaaaatcgt aattacctgt tgctcagcaa tcagtgattt aagacgcgta attaacgcag
    53881 ttttatcagc aaaatgttcg ccaacgccgc tggcggtgct gatagcatgg ctttgtttac
    53941 ccatgcttaa cacgcggtca ataccagcag cttttgccgc ctcgcccacc tgtacatggc
    54001 aggcttcgct ttcagcgccc agttccgcca tatcgcccac caccagcacg cggtagcccg
    54061 gcatttcagc cagtacctga acagctgcgg tcattgaacc gacattggcg ttgtaggagt
    54121 cgtcgagcag caactggttt tctgccagtt ggatggggaa cagacggcca ggaacagctt
    54181 tcagatttgc cagccccgct ttgatagcat caagcgttgc gcccacggac atggagagcg
    54241 cagcggctgc cagcgcattc gcaatattgt gacgccccgg caacggcagc agaacatcga
    54301 cgctaccggt tggggtttgt agggtaaatt ccataccgtg cgaggtcaca tggatattgg
    54361 tggcggtgaa atcgctgttg gcggcattgg gtgagaaacg ccacactttg cgtgagccaa
    54421 ttacgctctg ccagttcagc cagtcgttgt tgtcggcgtt cataatggcg ataccgtttt
    54481 ccggcaggcc gctaaagatt tcacctttcg ctttcgcgac acccgcaagc gagccaaaac
    54541 cttccagatg cgccgctgcc aggttgttga ccagcgccgc ttccgggcga gtcagactca
    54601 cagtccaggc aatctcgccc tgatggttcg cgccaagttc aataactgcg taatcgtatt
    54661 ccggcgttaa gcgcaacagc gtcatcggta caccgatgtc gttgttgaga ttgcctgccg
    54721 tataaagcgt gttgccgcac tggcttaaaa tcgccgccgt catctcttta acggaggttt
    54781 tgccggagga ccccgtcaga gcaaccacgc gcgccggaac ttgctggcga acccatgcag
    54841 ccagttcacc aaacgccaga cgcgtatcct tgacgattaa ctgcggcagg tcgatgtcca
    54901 gcggacggct aaccagtagt gcgcctgcgc cgccagcttt cgcctggtcg gcaaaatcgt
    54961 gggcatcaaa acgttcacct ttcagggcaa caaacaggca gcccggcgtc agttttcggg
    55021 tatcagtggt tacagcatca agggtgatat ctgcaccttg cagttcaccg ttgagaatat
    55081 cggtaagttg gctaagggtt acgctaatca tgcaatcacc cccagcagac gcgccaccgt
    55141 gacgcgatcg gagtagtcca gacgctgatt gccaacaatc tggtaatcct catggccttt
    55201 gcccgcgacc agtaccacat cattctcttt agcctgcata acggcgcaag tcaccgcttc
    55261 agcacggcct tccatcactt tggcatgtcc ggcatctaac attcccgcca gaatatcgtt
    55321 gatgatggca cgcggttctt cggtacgcgg gttatcgtcc gtcaccaccg ccacgtcagc
    55381 aaactcttcg gcaattgcgc ccatcagtgg acgcttacct ttatcgcgat cgccaccaca
    55441 gccaaagacg caccacagct tgcccgcaca gtgcaggcgc gccgcctgta aggctttttc
    55501 cagtgcatcc ggcgtatgcg cgtaatccac caccaccgtc ggtttacctg gcgcagtgaa
    55561 cacttccata cgtccgcaaa ccggttgcag acgcgcggcg gttttcagca gatcagccag
    55621 tggatagccc agcgccagca gtgttgccag cgcgagcaac aggttgctga cgttaaaagc
    55681 gcccatcaga tggctttcaa tttcgccatc gccccagctt gaactaaagc gaatggtcgc
    55741 gccgctgtcg tgatagttca cttcggtcgc tttcaaccag cgtccgtgac agttcggatt
    55801 gatatgatct tccattgata ccgcaaccgc gtccggcagt tttgccagcc agcggcggcc
    55861 cacttcatcg tcggcgttaa taatcgcctg accgcaatga tgttcagaat aaagcagcca
    55921 tttcgcagct tcgtagtgtt ccatatcacc atgataatca aggtgatcgc ggcttaagtt
    55981 ggtaaagacc gacgccgcaa atttcaatgc cgccacacgg tgctgtacca gcccgtggga
    56041 ggaaacttcc attgcgcaaa aagtcgcgcc ctgatccacc agccccgcca gctcatgctg
    56101 aacatcgact gccgaaccgg tagtattttc tgtcgggatc actttcccca gcagaccgtt
    56161 accaacggtg cccattaccg cgctggtttc gccaagcagt tggctccact gcgccagaag
    56221 ctgggtcgtc gtggttttgc cgttggtgcc cgttacgcct acgagacgta aattgtcaga
    56281 gggttcatgg taaaagcggc ccgccagtgc agataaacgc tcgttgagct ggctgagata
    56341 gatgaccggt acgccgtgca tttcacggat ttcaccgtcg gtcgcctcat ctttcgcctc
    56401 tgcaataatg gcagccacac cttgcgctat cgcctgcggg atatatcgac gcccgtccgc
    56461 ctgatgacct actacagcta caaagagatc gcccgccgca gccacacggc tgtcgagtgt
    56521 catctctcgc agtgctcgcg aaggtgcgtc tggcacccac ggagcaagaa ggtcgcgcaa
    56581 attacgatct gccacctgtc ccctcgcctt gattaatcac aaattcattt ttatcgcccg
    56641 ttgtcagcgc atccggctcg atgttcatgg tacgcaatac gccgcccatg atggtaccaa
    56701 agaccggcgc ggaaacggcg ccgccgtagt atttacccgc ctgcggatcg ttgataacaa
    56761 caaccagcgc gaagcgcggc tgactcgcag gcgcaacgcc tgcggtataa gcaatatatt
    56821 tattgatgta gcgaccgtcc ggcccgacct ttttcgcggt accggtttta atggcgatac
    56881 gatagccttt aatcgccgcc ttcacgccgc cgccgcctgg tagcgccacg ctttccatca
    56941 tatgcaccac ggtgcgaaca atggattccg ggaagacacg ttcaccggga accggggggt
    57001 caactttggt aatcgacagt gggcgataaa tgccgtagct gccgatagtt gcgtagactc
    57061 gcgctaactg taacggtgtt accattagcc cgtagccgaa agagaaggtg gccctctcta
    57121 tgtcagacca ccgttgtttt tgaggatata agccactgcg ttctccgacc aaccccaaat
    57181 tggtcgcttt tcccagtcca aaacgtgagt aagtatctac taacgctgag gacggcatcg
    57241 ctaacgccag cttggaaaca ccgacgttac tcgacttctg taataccccg gtcagggtta
    57301 attcgctgta gcgtgccacg tctttgattt cgtggccgtt aattcgataa ggaatggtat
    57361 tgagtaccga gttttcccgc accacgccac gttgcaacgc ggtcatcacc accatcggtt
    57421 taaccgttga gcccggttca aacacgtcgg tgatggtacg gttacgcatc gcctctttcg
    57481 gcgtgccgct cagattgtta gggttgtatg acgggctgtt agccatcgcc agcacttcac
    57541 ctgtgttgac atccaccagc acggcgctac cagattcagc cttgttaaag gccaccgcgt
    57601 tgttcagttc gcgataaacc agcgcctgca ggcgttcatc aatactcagc gccaggttgt
    57661 gcgctgcctg gctgtcagta gaagaaatat cttcaattac gcgaccatag cggtctttac
    57721 gcacaatgcg ctcacccggc tgcccggtaa gccatttatc gaaactcttc tcaacgccct
    57781 caatcccttg actatcgacg ttagtaaagc cgatgaggtg agcagtcact tcgccggacg
    57841 gatagtaacg gcgagactct tcacgcagat gaatccccgg cagtttcagt tttttgatgt
    57901 agtccgccat gtcagggttt acctgacgcg ccagataaat aaagcgccct ttcgggttgg
    57961 cgttaatgcg ggcagaaagc tgatccagcg gaatattgag cgcgttagcc agcgccttcc
    58021 agcggtcacc gacgctgata ccgccagcgt catgcacttc tttcgggtca gcccaaatcg
    58081 cttttaccgg cacgctcacc gctaacgggc gaccagaacg gtcagtaatc atgccgcggg
    58141 aggtggaaac ttgctgaacg cgaagagaac gcatgtcgcc ctctttcacc agcatatccg
    58201 gggagataac ttgtaaccac gctacgcgtc cgagcagaaa agccagcgcc aggagaatac
    58261 agccgcataa caacgcaaaa cgccaactga taaagttggc atgttcttcc tgacgttttg
    58321 gtttctgcgt tttcaccgct gctttcatgc gtcgcgttta tccttatttt tgcactacga
    58381 tattttcttg tgacggatca acatgctgca tttgcagctt ttccgtggcg atcctttcca
    58441 cccggctatg gtcgccgagc gcattctctt caaggatcag gttgcgccat tcaatgtcta
    58501 aagcatctcg ctccagcacc agttgttcgc gctgagcggt cagtaaacgg gtatggtgcg
    58561 ccgtggttac cacggtcacc gccgtcaaaa taatgcaaat gaacaggcag agtggcagct
    58621 tcccaaatcg caaaagatcg tcaccgataa caccaggcaa tgcatggcgc tcgtggcttc
    58681 ccatcgatcc tttaactttg cttagagctt ctgtcactct gctgatcatg cgttcgtcct
    58741 ctctgcaata cgcagaactg aactacgggc acgagggttc tcagccacct cttcttcgcc
    58801 cggcattaac ttgcctagtg ctcgcagctg acggccaccc agttttttga gctgctcttc
    58861 agtcatcggt aaccctgccg gaacttgcgg accgcggctg ttttcacgca taaaacgttt
    58921 cacaatacgg tcttccagcg agtggaagct gatgatcgaa agccgcccac ccggggccag
    58981 cacgttgagc gagcttttta gcgcctgctc tatctcctcc agttcactgt ttacccaaat
    59041 gcgcaccgcc tggaaggtac gggtcgcggg atgtttaaac ttatctttca ccggcgttgc
    59101 agcagccacg acttccgcca gttctttggt gcgggtcatc ggctgttcgc ggttacgctc
    59161 gacaatggcg cgggcaatgc gtttggcaaa acgctcttca ccataggttt tcaataccca
    59221 ggcgatatcg gcttcttctg cggtttgtag ccattcagcg gctgactgcc cacgggttgg
    59281 gtccatacgc atgtccagcg gaccatcgcg cataaaggaa aagccgcgtt cagcatcatc
    59341 aagttgcggt gaagagacgc caagatcgag gagaatgccg tcgatcttgc caataagatc
    59401 gcgctcggca acgtattcgc ccagcgcgga gaaaggtccg tggatgatgg agaagcgcgg
    59461 atcatcaata gtcttcgcaa cggcgatagc ctgcgggtcg cgatcgatcg ccagcaaacg
    59521 cccctcttcg ccaagctgcg agaggatcag acgtgagtga ccaccgcgac caaaagtccc
    59581 atcaatgtag atgccatcag gacggatatt gaggccatta acggcttcat ccagcagcac
    59641 cgtagtatgt ttatagtttt ccatcatttt atagagacaa gtcctgcagt cgctccgata
    59701 agtctccggt agccaactgc tctgcgtcga tatcttcctt gacctgttga tgccaggttg
    59761 tttcatccca cagctcaaac ttgttgaact gtccaaccag catcacttct tttgtcagcc
    59821 cggcatgttg ccgcagtact ggcgcgatta acaatcgacc tgcgccatcc atctgacatt
    59881 cgctggcatg acctaacagt aggcgctgca cacggcgctc aaccgggttc atgctcgaca
    59941 gacgcgataa tttttgctcg ataatttccc attcaggcag ggggtaaagc agcaggcacg
    60001 ggtgatgaat gtcaatggtg caaaccattt gaccggcagc gttctcaagc agctgttccc
    60061 gataacgggt aggcactgat aagcgccctt tgctgtcgag attgactaac gttgctcccc
    60121 ggaacatgcc agcctcaccc cttattccca ctttgcccca caatttccca ctgaaaggag
    60181 tttacggagc gtaggaaaag cttgtcaagc cagtcacaag gcataccgag gtaaaaaacc
    60241 cgatgtttac ggctaatatc agcgttgttt attttacgtc caacgtgcga agcaaattaa
    60301 cgttatgaat agctgtaaga aaaaatccct catcgtcatc actgttttaa aatgactcac
    60361 tattttccac agcaaatata aagtgtcagt ttgcgacgcg agcggcattt taggacatat
    60421 cttccccggt taacagtcct tgttacgtct gtgtggcgct tgatgccagc gggtttgcgc
    60481 aaaagcaaag aaaagtgttt gttaaatcgg caattcatcg ccgcatgtaa caaaataatg
    60541 caaaaacgcg ctgtcgatat cacttaatta ttgtgaattt cagctggtga gttaattaac
    60601 gggcgggttt aaaaccattt atcaataata gtcaggaata gtcttattta ctttaaggat
    60661 attgatgtcc agtcccgtac tctacgcgcc agagggaaat tcacctggcg cgtatttttg
    60721 ttcgcggctt agctacggct gagcacgccg cggcgataga gattacgttt aatgcgcgtt
    60781 aaaccaggtt ttggcttacg cggttcgtcc aggcttgcca ggacaatctc cagcacacgc
    60841 tctgcgacat cgcggtgacg ttgagccact gccagcaccg gacactgtaa gaagtcgagc
    60901 agttcgttat cgccaaaggt ggcaattgcc aggtcagaag gcagtttgcc gtcgcgacgc
    60961 agcgtgacat ccatcactcc ttgcaacaac gcaaacgacg ttgtgaacag cgcctgcggc
    61021 atcggatgcg tttccagcca tttttcgaat aactgggcag ccgcctcccg ctcatagctg
    61081 ttggcataca ggaaatgcac ttcgcgcgga tcatctttcc aagcagtacg gaaaccttgt
    61141 tcacgcagga agctgacaga aagctccggc agcgcgccaa gataaagcac cgtctcggcg
    61201 ggaaacttac gtaactcttc cgccagcatt tcggcatcat cctgatcggc accaaccacg
    61261 ctggtgaaat gttcacgatc gagggcgcgg tccagcgcga caatcgggaa cgggtcgtta
    61321 gcccagcgtt gataaaaagg atgctcaggt ggcaacgacg tcgaaacaat aatggcatca
    61381 acctgacgct gtaaaaggtg ctcaatgcag cgcatttcgt tgtctggctg atcttctgag
    61441 caggcaatca gcagttgata accccgttgc cgcgcctggc gttcaagata gttagcgatg
    61501 cgggtatagc tggtgttctc cagatcgggg atcacaagac caatagaacg tgtgcgtcca
    61561 gcacgaagcc cagctgccac ggcgttcggg tggtaattgt gctcacgcac cacagccatg
    61621 actttttcaa cggttttgtc gctcacacgg tattgcttcg ctttgccgtt aataacatag
    61681 cttgcagtgg tccgcgacac tcccgccagc cgagcgattt catccagttt cacaattgcc
    61741 ccttgcgtaa aatgtaaaaa ccatagccat tgtactggta tgggttaaat tcattaacat
    61801 ctaagcgcag aaaattaact gcggcaactg cttttattcc aggttcccac tgatttcgca
    61861 aaaaaagccc aacgtgacat gttgggctgc aaattgcgca ctgagatcat tctcaacgca
    61921 ttattttatc gccgcgcgaa agtccgacca caccagagcg agcaacttcc acaattttcg
    61981 ccacatcgcg aatcgatgct aaaaatgcat caagcttatc gctggtgcct gctaattgaa
    62041 cggtataaag cgagggggtg acatcgataa tttgcccacg gaatatttcc gtattacgtt
    62101 tcacttcgtc acgcccgtaa ccgctggcct gaattttcac cagcatgatt tcccgctcaa
    62161 catgcgcgcc ctgccccaac tcactcacgc gcaagacatc gaccagcttg tgtaattgct
    62221 tttcgatctg ctcaagtact ttttcatcgc ccacggtctg gatggtcata cgcgataatg
    62281 tctgatcgtc ggttggcgca acggtcaggc tttcaatgtt gtagcctcgc tgggaaaaaa
    62341 ggccaatcac gcgggataac gcgcctgatt cattttcgag taagactgat aatatccggc
    62401 gcataatcag gttctctccg ttttgcttaa ccacatttca tccattccgc ccccgcgaat
    62461 ctgcatcggg tagacgtgct cactgccatc gacggtaaca tcaacaaaca ccaggcgatt
    62521 attgcgcacc tgttccagcg cctcgctaag tttgctttcc agctcatgcg gatgagagat
    62581 ctggatcccg acgtgaccat aggcttccgc cagacggacg aaatcgggta gtgattgcat
    62641 ataagattgt gaatgacggc cggaatagat catgtcctgc cactgtttca ccatccccag
    62701 atagcggtta ttgagattca ccaccagtac gggcaactcg tattgcaacg cggtagacag
    62761 ttcctggatg ttcatctgaa tactgccgtc gccagtgacg caaaccacgg tttcttctgg
    62821 caacgccatt ttgacgccca gtgccgcagg taaaccaaaa cccatcgtgc cgagaccgcc
    62881 ggaattgatc cagcgacgcg gtttgtcgaa tggataataa agcgcagcaa acatctggtg
    62941 ctgcccgaca tcggacgtca cgtaagcgtc cccattagtc aaccgccaaa gagtctcgat
    63001 caccgcctgc ggtttaatct tttcactgtg agtgtcatat ttcaggcact gaagagcgcg
    63061 ccactgttca atctgctgcc accagtcgcg gatctcatcc agcggttgat gggcggattc
    63121 ttgcgacagg agttcaagca tttgttcgag gacctggcga gcatccccca caatcgggat
    63181 atccgcagtc acggttttag aaatggaagt aggatcaata tcgatatgca gaacagtggc
    63241 atttgggcag tactttgcca gattgttcgt cgttcggtca tcaaatcgta ccccgacggc
    63301 gaaaatcaca tccgcgttat gcatcgtcat attggcttcg taggtaccgt gcattcccag
    63361 cattcccagt gcctgacgat gcgttgccgg aaacgccccc agccccatca atgaggaaac
    63421 aaagggcaga ttcaacgcct ccaccgtttc tttcaactgc tgatggcagc ccgccatgat
    63481 tgccccaccg cctacgtaga caaccggttt ttttgccgcg accagcgttt gcagagcacg
    63541 cttaatttgc cctttatgtc cggtagtagt gggattgtaa gaacgcatac tgaccgactc
    63601 agggcagaca tagggtaatt tgttcgccgg attaagaata tctttcggta aatcaacgac
    63661 cactggtcct gggcgaccac ttgccgccag ccagaaagcc tttttcagca cctgcggaat
    63721 gtcttccgtt tgcttaacca gaaaactgtg tttaaccacc ggtcgcgaaa tccccaccat
    63781 gtcgcactcc tgaaaggcat cgtaacctat caacgaggtc gctacctgcc cggaaaggac
    63841 aactaatgga atggaatcca tataagcggt ggcgatgccg gtaatcgcat tggtcgcccc
    63901 tggacccgac gttaccagca cgacgccgac ttccccggtc gcacgcgcca ggccatcggc
    63961 catatgcacc gccgcctgct catgacgaac taatacatga tcaataccac ccacggtatg
    64021 caatgcatcg taaatatcaa ggactgcgcc tccgggataa ccgaatactt gtttaacgcc
    64081 ctgatcgata agcgatcgga cgaccatctc ggctccagac aacatctcca tggcctgcct
    64141 cactgtttga cggaaaaaat gtgtaaaaca cattaaccgt ccgatcttgc ttaagcaatt
    64201 ggcagccaga gacataaacg cagggtaata agaataaaca ggaggaaagg tgaaaagaaa
    64261 cgataaaatc ctccattacg aaggattaaa ttgataataa atcggctgaa tcccacaact
    64321 tacagcaatt tctctatttt tttaattaat cctaaaatag agagtccaca cattcagaat
    64381 aaaattctac attcaataaa aaatgcagaa taaaccagac attcatgtct ggcctattct
    64441 gcattcagtt agcgtttgca aattgagact aattgttctt ccatccactg atggccttta
    64501 tcgcgcccgg cagcttcatg ccaggagaga taacaggttc tgctgttttg ttttaacggc
    64561 agcggtaata cctgtaattc taaggattca gcgaactctt cagccagcca acgcggcgca
    64621 atagcgacca aatgcgtttg cgacaccacg ctaagtacgc tcatcattgc catgccctga
    64681 tacgcgatac tggcttgctt atctaccgtg tcataccaag gttgactaaa tgacgcgaaa
    64741 cgatcgagcg aaaccgccgc atgttgttcg ttataaacat catgtttcag taacgggcct
    64801 ttaattgttg gatgattttt gctggctacc agcaccattt catctttaaa taatggcacg
    64861 ctggtaaatt caggacgatg gaagtcttca taactaatca caaactccgt ttcctgataa
    64921 cgcagctgat gttcagtgtt ctgatttaat gaagacttga acataacatg tatatttggc
    64981 gcaatctgct caatgtgatt ataaatctgc gaggtcagaa tgctgtctaa cgggctgcaa
    65041 acacaaagat gaaatacacg ttcactgctc gcgggttcaa aacctgaact aggcaattca
    65101 ttttgtacta gttgcaatgc ctggcgaact gaaccaaaaa gttgaaatgc gcgagcagtc
    65161 ggttgaatac cacggccata acgaacaaaa agctcgtcat taaacatcac cttcaggcgt
    65221 gcaacagcgt tactgaccgc aggttgcgac atacccagaa catgagcggc acgggtaatg
    65281 ttttgctcct gcatcacggc atcgaaaacg gttaataagt tgagatcgac catgcgtagc
    65341 tgtggcttgc ttaactccgc cgtctctgaa tgatctgttt gtacctctgg catacttaac
    65401 tccactgtca cacttaactc cctttccctt attggaatgc agagaaataa tcctgaagaa
    65461 tatgatttat catgcatata aaataagaaa aagcaaaatg agtaaaattc ggaaaacata
    65521 aagatacggg ttctgataaa aacagaatcc attgccgtgg aatgagtcat ttaccacagc
    65581 ataataatcc ataatgtttt tgttgcgaaa acaatctaat cataactacc gcgaatactc
    65641 aatcatctac aaaatggatt aaatgtgctt atttaataat taatttatat atttaatgca
    65701 ttaattctta acattaattg atcaataata ttcaccaaat caatatcaaa aaaaatcgca
    65761 aaacatataa ttcaatacaa atcatcagga taggttttgc aacgcgtgca ttttgtcccc
    65821 tttttcctcg ttgattagat gcaaaaattt atgctgaaat atgtcaaccg atgaaaagcg
    65881 tcggtagtta agcagaaatt aatatcgctt actttaacca ccgtagcaca attagctaat
    65941 tttacggatg cagaactcac gctggcggga cgtttttatt gcgtcagggt tgacatccgt
    66001 ttttgtatcc agtaactcta aaagcatatc gcattcatct ggagctgatt taatgactca
    66061 catcgttcgc tttatcggtc tactactact aaacgcatct tctttgcgcg gtagacgagt
    66121 gagcggcatc cagcattaag ccagcacgca gtcaaacaaa aaacccgcgc cattgcgcgg
    66181 gtttttttat gcccgaagcg aggcgctcta aaagagacaa ggacccaaac catgagccag
    66241 caagtcatta ttttcgatac cacattgcgc gacggtgaac aggcgttaca ggcaagcttg
    66301 agtgtgaaag aaaaactgca aattgcgctg gcccttgagc gtatgggtgt tgacgtgatg
    66361 gaagtcggtt tccccgtctc ttcgccgggc gattttgaat cggtgcaaac catcgcccgc
    66421 caggttaaaa acagccgcgt atgtgcgtta gctcgctgcg tggaaaaaga tatcgacgtg
    66481 gcggccgaat ccctgaaagt cgccgaagcc ttccgtattc atacctttat tgctacctcc
    66541 ccaatgcaca tcgccaccaa gctgcgcagc accctggacg aggtgatcga acgcgctatc
    66601 tatatggtga aacgcgcccg taattacacc gatgatgttg aattttcttg cgaagatgcc
    66661 gggcgtacac ccattgccga tctggcgcga gtggtcgaag cggcgattaa tgccggtgcc
    66721 accaccatca acattccgga caccgtgggc tacaccatgc cgtttgagtt cgccggaatc
    66781 atcagcggcc tgtatgaacg cgtacctaac atcgacaaag ccattatttc cgtacatacc
    66841 cacgacgatt taggcctggc ggtcggcaac tcactggcgg cggtacatgc cggtgcacgc
    66901 caggtggaag gtgcaatgaa cgggatcggc gagcgtgccg gtaactgttc gctggaagaa
    66961 gtcatcatgg cgattaaagt tcgtaaggat attctcaacg tccacaccgc cattaatcac
    67021 caggagatat ggcgcaccag ccagttagtt agccagattt gtaatatgcc gatcccggca
    67081 aacaaagcca ttgttggcag cggcgcattc gctcactcct ccggtatcca ccaggatggc
    67141 gtgctgaaaa accgcgaaaa ctacgaaatc atgacaccag aatctattgg tctgaaccaa
    67201 atccagctga atctgacctc tcgttcgggg cgtgcggcgg tgaaacatcg catggatgag
    67261 atggggtata aagaaagtga atataattta gacaatttgt acgatgcttt cctgaagctg
    67321 gcggacaaaa aaggtcaggt gtttgattac gatctggagg cgctggcctt catcggtaag
    67381 cagcaagaag agccggagca tttccgtctg gattacttca gcgtgcagtc tggctctaac
    67441 gatatcgcca ccgccgccgt caaactggcc tgtggcgaag aagtcaaagc agaagccgcc
    67501 aacggtaacg gtccggtcga tgccgtctat caggcaatta accgcatcac tgactataac
    67561 gtcgaactgg tgaaatacag cctgaccgcc aaaggtcacg gtaaagatgc gctgggtcag
    67621 gtggatattg tcgccaacta caacggtcgc cgcttccacg gcgtcggcct ggccaccgat
    67681 attgtcgagt cctccgccaa agccatggtg cacgtactga acaatatctg gcgtgccgca
    67741 gaagtcgaaa aagagttgca acgcaaagct caacacaacg aaaacaacaa ggaaaccgtg
    67801 tgatgtcgaa gaattaccat attgccgtat tgccggggga cggtattggt ccggaagtga
    67861 tgacccaggc gctgaaagtg ctgaatgccg tgcgcaaccg ctttgcgatg cgcatcacca
    67921 ccagccatta cgatgtaggc ggcgcagcca ttgataacca cgggcaacca ctgccgcctg
    67981 cgacggttga aggttgtgag caagccgatg ccgtgctgtt tggctcggta ggcggcccga
    68041 agtgggaaca tttaccacca gaccagcaac cagaacgcgg cgcgctgctg ccgctgcgta
    68101 agcacttcaa attattcagc aacctgcgcc cggcaaaact gtatcagggg ctggaagcat
    68161 tctgtccgct gcgtgcagac attgccgcta acggcttcga catcctgtgc gtgcgcgaac
    68221 tgaccggcgg catctatttc ggtcagccaa aaggccgcga aggtagcgga caatatgaaa
    68281 aagcgtttga taccgaggtg tatcaccgtt ttgagatcga gcgtattgcc cgcatcgcgt
    68341 ttgaatctgc tcgtaaacgt cgccacaaag tcacctcaat cgacaaagct aacgtgctgc
    68401 aatcctctat tttatggcgt gaaatcgtca acgagatcgc cacggaatac ccggatgtcg
    68461 aactggcgca tatgtacatc gacaacgcca ccatgcagct gattaaagat ccatcacagt
    68521 ttgacgttct gctgtgctcc aacctgtttg gcgacattct gtctgacgag tgcgcaatga
    68581 tcactggctc gatgggcatg ttgccttccg ccagcctgaa cgagcaaggt tttggactgt
    68641 atgaaccggc gggcggctcg gcaccagata tcgcaggcaa aaacatcgcc aacccgattg
    68701 cacaaatcct gtcgctggca ctgctgctgc gttacagcct ggatgccgat gatgcggctt
    68761 ccgccattga acgcgccatt aaccgtgcat tagaagaagg cattcgcacc ggggatttag
    68821 cccgtggcgc tgccgccgtt agtaccgatg aaatgggcga tatcattgcc cgctatgtgg
    68881 cagaaggggt gtaatcatgg ctaaaacgtt atacgaaaaa ttattcgacg ctcacgtagt
    68941 gtacgaagcc gaaaacgaaa ccccgctgtt atatatcgac cgccacctag tgcatgaagt
    69001 gacctcaccg caggcgttcg atggtctgcg cgcccacggt cgcccggtac gtcagccggg
    69061 caaaaccttc gccactatgg atcacaacgt ctctacccag accaaagaca ttaatgcctg
    69121 tggtgaaatg gcgcgcatcc agatgcagga gctgattaaa aactgcaaag aatttggcgt
    69181 cgagctgtat gacctgaatc acccgtatca ggggatcgtc cacgtaatgg ggccggaaca
    69241 gggcgtcacc ttgccgggga tgaccattgt ctgcggcgac tcgcataccg ccacccacgg
    69301 cgcgtttggc gcactggcct ttggtatcgg cacttccgaa gttgaacacg tactggcaac
    69361 acaaaccctg aaacagggcc gtgcgaagat catgaaaatt gaagtccagg gcaaagccgc
    69421 gccgggcatt accgcaaaag atatcgtgct ggcaattatc ggtaaaactg gcagcgcagg
    69481 cggcaccggg catgtggtgg agttttgcgg cgaagcaatt cgtgatttaa gcatggaagg
    69541 tcgtatgacc ctgtgcaata tggcgatcga aatgggcgca aaagccggtc tggttgctcc
    69601 tgacgaaacc acctttaact atgtcaaagg ccgtctgcat gcgccgaaag gcaaagattt
    69661 cgacgacgcc gttgcctact ggaaaaccct gcaaaccgac gaaggcgcaa ctttcgatac
    69721 cgttgtcact ctgcaagcag aagaaatttc accgcaggtc acctggggca ccaatcccgg
    69781 ccaggtgatt tccgtgaacg acaatattcc cgatccggct tcgtttgccg atccggttga
    69841 acgtgcgtcg gcagaaaaag cgctggccta tatggggctg aaaccgggta ttccgctgac
    69901 cgaagtggct atcgacaaag tgtttatcgg ttcctgtacc aactcgcgca ttgaagattt
    69961 acgcgcggca gcggagatcg ccaaagggcg aaaagtcgcg ccaggcgtgc aggcactggt
    70021 ggttcccggc tctggcccgg taaaagccca ggcggaagcg gaaggtctgg ataaaatatt
    70081 tattgaagcc ggttttgaat ggcgcttgcc tggctgctca atgtgtctgg cgatgaacaa
    70141 cgaccgtctg aatccgggcg aacgttgtgc atccaccagc aaccgtaact ttgaaggccg
    70201 ccaggggcgc ggcgggcgca cgcatctggt cagcccggca atggctgccg ctgctgctgt
    70261 gaccggacat ttcgccgaca ttcgcaacat taaataagga gcacaccatg gcagagaaat
    70321 ttatcaaaca cacaggcctg gtggttccgc tggatgccgc caatgtcgat accgatgcaa
    70381 tcatcccgaa acagtttttg cagaaagtga cccgtacggg ttttggcgcg catctgttta
    70441 acgactggcg ttttctcgat gaaaaaggcc aacagccaaa cccggacttc gtgctgaact
    70501 tcccgcagta tcagggcgct tccattttgc tggcacgaga aaacttcggc tgtggctcct
    70561 cgcgtgagca cgcgccctgg gcattgaccg actacggttt taaagtggtg attgcgccga
    70621 gttttgctga catcttctac ggcaatagct ttaacaacca gctgctgccg gtgaaattaa
    70681 gcgatgcaga agtggacgaa ctgtttgcgc tggtgaaagc caatccgggg atccatttcg
    70741 acgtggatct ggaagcgcaa gaggtgaaag cgggagagaa aacctatcgc tttaccatcg
    70801 atgccttccg ccgccactgc atgatgaatg gtctggacag catcggcctg accctgcagc
    70861 acgacgacgc tattgcgtct tatgaagcaa aacaacctgc gtttatgaat tagacccctt
    70921 gcccggtcaa atgaccgggc tttccgctat cgtccacgtc atcaaacgtc tttaaccttt
    70981 gcggttaaaa ataatgcgac aacagaaata atcgcaatta cccagtagac agcaaagtgc
    71041 ccccaacttt gtgcaattgc tccctgaata acaccagcca gaattacccc ggtagagata
    71101 gtgttggtaa ataaggtggt agctgccccc gctcttccag gcattaaatc ctgaaaccat
    71161 agcatcccaa tacccgcaac aatgccgata aatacagcgt taaaaagttg cagcgtcatc
    71221 aacgccatac ggctatgaaa gaaaatcaat ccagtgtaaa acagcactcc tgccaccact
    71281 gcaatgacca tcattcgccg cttaccataa cgtttgacat agtagccagc cagaatcatt
    71341 gctggtattt ccagtccagc tgccgtcccc atcaggaaac ccgccagttt gtctggcaat
    71401 cctaaatcgc tactgatcca caacggcata tcaataatgt acatagtgtt gcaggtccac
    71461 attaacgtcg aggcgacaaa taacatccgt acgttactat cctgccagtc accttgcatt
    71521 gataaagcat tttccgacgg cagttctacc cgcgccacag acggaagcat aaatgcaatc
    71581 aataccagac tgagtgtgaa tatcccggcg gcaatcgaaa acatcactgt aaagccgtaa
    71641 ttcaacgcca gcataaaggc cagcggtgga ccgattaccc atgccagaga aagctgcgca
    71701 cgcatcaccg agctaaacat caccacttct cgcgccgagt tatccgcgta ttcccgcgcc
    71761 agagcaaata actgtggcat tgccgtattg gccagagatg ccagaagcac accacaggtg
    71821 ataagcgtca gataatgacg attaaatgca aacagtagcg cattgccaat agccatcaaa
    71881 cagcaaaata taatcagttt tcgccgatcg ccctgactgt cagaacgttt tgccaaccag
    71941 aggcttacgc cgatcccagc aatagcattc accgtataaa agagtccgat ccagaaaggt
    72001 tgcgcgccaa cctcacgact cagaaataag ctcaatgtag gagcctgtag cgccccggcc
    72061 acccccatca taaaagcgac cagcataaat gccgcgtaaa caccgttcat acggcgagcc
    72121 atcgtcatta tccagatcat acgttccctt tttagcgcgg cgagaataaa aaaaaccagc
    72181 aggtataatc tgctggcggg tgattttaca ccaatactca gtcacacatg atgcaggcaa
    72241 gtcaactttc agaattgcgg tcatcccact gcatcagtcc ttcaagcatg gttaatcgtt
    72301 gtgggacgct taaccaacgc aaccagcaca acttcgctgt cgcggtaaaa tagtgctgat
    72361 aaaactgacg catggggcac ctccttgctt catcgttagg aattattgac ttaatatagg
    72421 gaaaataaaa ttgctgtctt ttgcgcagga gttccccttt tatgccatct gctcgtctgc
    72481 aacaacagtt catccgcctg tggcaatgct gcgagggtaa atcgcaggac acaacgctca
    72541 acgaactggc agcgttattg agctgctcgc gtcgtcatat gcgtaccctg ctcaacacca
    72601 tgcaggatcg cggctggctg acgtgggaag cggaagtcgg gcgcggtaaa cgctcgcgtc
    72661 tgacattcct ctataccggg ctggcgcttc agcaacagcg ggcggaagac ctgctggagc
    72721 aggatcgtat cgaccaacta gtgcagttgg ttggcgacaa agcgactgtg cggcaaatgc
    72781 tggtttctca tctgggccgc agcttccgcc aggggcggca catcctgcgc gtgctctact
    72841 atcgtccgtt gcgtaatctg ctacctggca gcgcattgcg ccgttccgaa acccatattg
    72901 cccggcaaat cttcagttcg ctaacgcgca taaatgagga aaatggggaa ctggaagcag
    72961 acatcgccca ccactggcag caaatatcac cgcttcactg gcgtttcttt ttgcgtccag
    73021 gagtccattt tcatcatggt cgtgaactgg aaatggacga cgtgatcgcc tctttaaaac
    73081 gaatcaatac gctgccgctc tattcgcata ttacggacat tgtgtcgccg acgccctgga
    73141 cgctggatat ccatctcacg cagccggacc gctggttgcc gttactgctg gggcaagttc
    73201 cggcgatgat cctgccgtgc gaatgggaaa ccctcagtaa ctttgccagc catcctattg
    73261 gcaccggtcc gtatgcggtg attcgcaaca gcaccaatca actgaaaatt caggcattcg
    73321 atgacttctt cggttaccgg gcattaatcg acgaagttaa cgtctgggtt ctgccggaaa
    73381 ttgccgacga gccagccggt ggtctgatgc taaaaggtcc acagggcgag gaaaaagaga
    73441 ttgaaagccg cctggaggaa ggttgctact atttactgtt cgacagccgc acccatcgcg
    73501 gggcgaatca gcaagtcagg gactgggtaa gctatgtgct ttctccaact aatctggtct
    73561 atttcgctga ggaacagtac cagcaactgt ggttcccggc ttatggactg ctcccccgtt
    73621 ggcaccatgc ccgcaccata aagagcgaaa aaccggctgg cctggaaagc ctcaccctga
    73681 ccttttatca ggatcacagt gagcatcggg tgattgccgg gatcatgcag cagattctgg
    73741 caagtcacca ggtcacgctg gaaatcaaag agatcagcta cgatcagtgg catgaaggag
    73801 agatcgagag cgatatctgg cttaatagcg ccaactttac gctgccgctg gatttttcgc
    73861 tgttcgcgca cttgtgcgag atgccgctgc tccaacactg tattccgatc gactggcaag
    73921 ccgacgccgc ccgctggcgc aatggcgaaa tgaacctggc gaactggtgc cagcaactgg
    73981 tcgccagtaa agcaatggtg ccgcttatcc accactggct gatcattcag ggacaacgca
    74041 gtatgcgcgg cctgcgtatg aataccctcg gctggttcga ttttaaatca gcgtggtttg
    74101 cgccaccgga tccatgattg ctggtagata acaaaatcac tacactaacg ccgttctcaa
    74161 cggggtgcca cgcaaacgcg tgcgctgaga aaatacccgt cgaacctgat ccggataacg
    74221 ccggcgaagg gatttgaggc tccttctcaa gtcctttgcc actctttttt gaggtgcaaa
    74281 gtgttaaaaa aatgtcttcc cctgctgttg ctgtgcacag cgcccgtttt cgctaaaccc
    74341 gttctgactg tttataccta cgattccttc gccgccgact gggggccagg tccaaagatc
    74401 aaaaaagctt ttgaagccga ctgtaactgc gaactgaaac tggtggcgct ggaagatggc
    74461 gtttcgcttc tcaaccgtct acggatggaa ggcaaaaata gtaaagccga tgtggtgctg
    74521 gggctggata acaatctgtt agacgccgcc agtaaaaccg gactatttgc caaaagcggt
    74581 gtggcagcgg atgccgttaa cgttcccggc ggctggaata atgacacttt cgtcccgttt
    74641 gattacggct atttcgcctt cgtctatgac aagaacaaac tgaaaaaccc gccgcaaagc
    74701 ctgaaagaat tggttgagag cgatcagaac tggctggtga tttatcagga tccgcgtacc
    74761 agcacgccgg ggctgggtct gctgttgtgg atgcaaaaag tctatggcga tgacgcccca
    74821 caagcctggc aaaaactggc gaagaaaacc gtcacggtca ccaaaggctg gagcgaagcc
    74881 tacggcctgt ttttaaaagg tgaaagcaat ctggtactga gttacaccac ctctccggct
    74941 tatcacattc tcgaagagaa aaaagataac tacgccgccg cgaactttag cgaaggtcac
    75001 tatctgcagg tggaagtcgc cgcccgcacc gctgccagca agcagccgga gctggcgcaa
    75061 aaattcctcc agtttatggt ttctccggct tttcagaatg cgatcccaac cggtaactgg
    75121 atgtatccgg tggcaaatgt cacgctgcct gccgggtttg aacaattgac caaaccggcc
    75181 accacgctgg agttcacacc agctgaagtg gcggcacaac gtcaggcatg gattagcgaa
    75241 tggcaacgcg ccgtcagccg ttaattcccg gctggttaat tccaggtgta agcgccgcca
    75301 cgctggtggt agcggttgcg atcgcggcgt ttctcgccct gtggtggaac gcgccgcagg
    75361 gtaactgggt ggcagtctgg caggacagct acctgtggca tgtggtgcgc ttctccttct
    75421 ggcaggcgtt tctctcggcg ctactctctg tcgtacccgc gatattcctc gcccgcgccc
    75481 tctatcgcag gcgctttccg ggtcggctgg cgctgttgcg tctgtgcgca atgaccttga
    75541 tcctcccggt gctagttgct gttttcggca ttcttagcgt ctatggtcgt cagggctggc
    75601 tagcatcgct ctgccaatcg ctcggtctgg agtggacctt ttcgccctac ggcctgcaag
    75661 gcattttgct ggcgcacgta ttttttaatc tgccgatggc gagccgctta ttactccagg
    75721 cactggaaaa cattcccggc gaacaacgtc agcttgccgc ccagcttggg atgcgcggct
    75781 ggcatttttt ccgcttcgtc gaatggccgt ggctacggcg acaaatcccg ccggttgccg
    75841 cgcttatctt tatgctctgt ttcgccagct tcgccaccgt gctatcgctg ggcggcggtc
    75901 cgcaggcaac cactatcgag ctggctattt atcaggcgct gagttacgac tacgatcctg
    75961 cccgcgcggc gatgctggcg ctgctccaga tggtgtgttg ccttgggctg gtgctgttga
    76021 gtcagcgact aagtaaggcc attgcgcccg gcaccacgct gctgcaaggc tggcgcgatc
    76081 cggacgatcg tctgcatagc cgcatttgcg acacggtgtt aatagtgctg gcgctgctgc
    76141 tgttgctgcc accgttgctg gcggtgatcg tcgatggggt aaatcgccag ttgccggaag
    76201 tgctggcaca accggtgctg tggcaggcgc tgtggacctc gttgcgtatt gcgctggcgg
    76261 caggtgtatt gtgcgtagtg ctgaccatga tgctgctatg gagcagtcgc gaactgcggg
    76321 cgcggcagaa aatgctggcg ggtcaggcgc tggagatgag cggcatgttg atcctcgcca
    76381 tgccggggat tgtgctggct accggcttct ttttactgct caacaacact atcggcctgc
    76441 cacaatctgc tgacggcatt gtgattttca ccaatgcgtt aatggcgatc ccttatgcgc
    76501 tgaaagtact ggaaaacccg atgcgcgata tcaccgcccg ctacagtatg ttgtgtcagt
    76561 cgctgggcat tgaaggctgg tcgcgcttaa aagtggtcga gctgcgcgcc ctgaaacgtc
    76621 cactggcgca ggcgctggct tttgcctgcg tgctgtcgat tggtgatttt ggcgtagtgg
    76681 cgttgttcgg taacgatgat ttccgtaccc tgccgtttta tctctaccag caaattggct
    76741 cctatcgcag ccaggacggc gcggtcaccg cgttaattct gctactactc tgttttctgc
    76801 tgtttaccgt gattgaaaaa ctaccggggc gaaatgttaa aactgactga tatcacctgg
    76861 ctttatcacc atttgccgat gcgttttagc ttaacggtag aacgcggcga gcaggtggca
    76921 atcctcgggc caagcggcgc gggtaaaagt accctgctga atttgattgc cggttttctg
    76981 acgccagcca gcggttcgct gactatcgat ggcgttgatc acaccacgac gccgccgtca
    77041 cgccgtccgg tgtcgatgct gtttcaggag aacaacctgt tcagccacct gacggtcgcg
    77101 cagaacatcg ggctggggct aaatccgggg ttgaaactga acgcggcaca gcaggagaaa
    77161 atgcacgcga ttgcccgtca gatggggatt gataatttaa tggcgcggtt accgggcgag
    77221 ctttccggcg gtcagcgaca gcgcgtggcg ttagcgcgtt gtctggtgcg cgaacagccg
    77281 attttattgc tcgatgaacc gttctctgcg ctcgatccgg cgttacgtca ggagatgctg
    77341 acgctggtga gcacgagctg ccagcagcaa aaaatgacgc tattgatggt gtcgcacagt
    77401 gtggaagatg cggcgcggat cgccacacgc tcggtggtgg tcgccgacgg gcgcatcgcc
    77461 tggcagggta agactgaaga attgttgagc ggtaaggcga gtgcttcggc aatattgggg
    77521 attacgggtt agtatgcact gtcagatgcg gcgtgaacac cttatccgac ctacggacag
    77581 tgcgttttgt aggcctgata agacgcggtc agcgtcgcat gaggcaaccc gcacaagaca
    77641 atcccctaaa ccccaaccac tttacgcaaa atatcgatat acaccggcat caacgggtgg
    77701 cgaattaaca ccaccagcgc caccacgccg atggcagaaa tcaacggcgt cagccacaac
    77761 aaacggccgc gcgacaaata atgacttaaa cgatcggttg ctttaccgct gcgccataac
    77821 cgccagcaca gccagccacc aacccacaaa aataccgccg tcgccagcag caaccattta
    77881 aactcgccgc tctgcattcc ggctggaata tcgatcgccg cgcccgccag aatcccaggc
    77941 aggaagtaaa acggtggcca cagcaggcag ccgataatat tcggcgtaat gaattttgcc
    78001 accggcagat ccagcattcc cgccaccatt ggcaccagcg gacgcgtcgg gccaacaaag
    78061 cgaccgacca gaatggtgaa catgctgtgt tgatgcaacg catgttcagt tttatcgagt
    78121 aatgctttgt ttttcttcag aaatgaccag cgatgcaacg gctttttaaa acgccagccc
    78181 agccagaagg agatccagtc gcccagcaga caaccgacaa tccctgccag ccaggcgtgc
    78241 cagaaactta actcgccgct gccaatcagc gctcccagcc ccgccatcag caccgtaccg
    78301 ggtagaatca aaccgaccag cgccagcgac tccagaaagg ccaccaacac caccgccatc
    78361 aatgaataca cggtagattg ggtaataaag tgttccagca atgcttgcat aatgtgcctg
    78421 tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt
    78481 ctgattcgtt actaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc
    78541 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag
    78601 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa
    78661 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa
    78721 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct
    78781 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac
    78841 acgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa
    78901 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt
    78961 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa
    79021 acccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg
    79081 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacaaccgt agtgatgaat
    79141 ctctcctggc gggaacagca aaatatcacc cggtcggcag acaaattctc gtccctgatt
    79201 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg
    79261 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag
    79321 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt
    79381 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca
    79441 ctgcgtcttt tactggctct tctcgctaac ccaaccggta accccgctta ttaaaagcat
    79501 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca
    79561 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt
    79621 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc
    79681 tccatacccg tttttttgga tggagtgaaa cgatggcgat tgcaattggc ctcgattttg
    79741 gcagtgattc tgtgcgagct ttggcggtgg actgcgccag cggtgaagag atcgccacca
    79801 gcgtagagtg gtatccccgt tggcaaaaag ggcaattttg tgatgccccg aataaccagt
    79861 tccgtcatca tccgcgtgac tacattgagt caatggaagc ggcactgaaa accgtgcttg
    79921 cagagcttag cgtcgaacag cgcgcagctg tggtcgggat tggcgttgac agtaccggct
    79981 cgacgcccgc accgattgat gccgacggta acgtgctggc gctgcgcccg gagtttgccg
    80041 aaaacccgaa cgcgatgttc gtattgtgga aagaccacac tgcggttgaa gaagcggaag
    80101 agattacccg tttgtgccac gcgccgggca acgttgacta ctcccgctat attggcggta
    80161 tttattccag cgaatggttc tgggcaaaaa tcctgcatgt gactcgccag gacagcgccg
    80221 tggcgcaatc tgccgcatcg tggattgagc tgtgcgactg ggtgccagct ctgctttccg
    80281 gtaccacccg cccgcaggat attcgtcgcg gacgttgcag cgccgggcat aaatctctgt
    80341 ggcacgaaag ctggggcggc ttgccgccag ccagtttctt tgatgagctg gacccgatcc
    80401 tcaatcgcca tttgccttcc ccgctgttca ctgacacctg gactgccgat attccggtgg
    80461 gcaccttatg cccggaatgg gcgcagcgtc tcggcctgcc tgaaagcgtg gtgatttccg
    80521 gcggcgcgtt tgactgccat atgggcgcag ttggcgcagg cgcacagcct aacgcactgg
    80581 taaaagttat cggtacttcc acctgcgaca ttctgattgc cgacaaacag agcgttggcg
    80641 agcgggcagt taaaggtatt tgcggtcagg ttgatggcag cgtggtgcct ggatttatcg
    80701 gtctggaagc aggccaatcg gcgtttggtg atatctacgc ctggtttggt cgcgtactcg
    80761 gctggccgct ggaacagctt gccgcccagc atccggaact gaaagcgcaa atcaacgcca
    80821 gccagaaaca actgcttccg gcgctgaccg aagcatgggc caaaaatccg tctctggatc
    80881 acctgccggt ggtgctcgac tggtttaacg gtcgtcgcac gccaaacgct aaccaacgcc
    80941 tgaaaggggt gattaccgat cttaacctcg ctaccgacgc tccgctgctg ttcggcggtt
    81001 tgattgctgc caccgccttt ggcgcacgcg caatcatgga gtgctttacc gatcagggga
    81061 tcgccgtcaa taacgtgatg gcgctgggcg gcatcgcgcg gaaaaaccag gtcattatgc
    81121 aggcctgctg cgacgtgctg aatcgcccgc tgcaaattgt tgcctctgac cagtgctgtg
    81181 cgctcggtgc ggcgattttt gctgccgtcg ccgcgaaagt gcacgcagac atcccatcag
    81241 cccagcaaaa aatggccagt gcggtagaga aaaccctgca accgcgcagc gaacaggcac
    81301 aacgctttga acagctttat cgccgctatc agcaatgggc gatgagcgcc gaacaacact
    81361 atcttccaac ttccgccccg gcacaggctg cccaggccgt tgcgactcta taaggacacg
    81421 ataatgacga tttttgataa ttatgaagtg tggtttgtca ttggcagcca gcatctgtat
    81481 ggcccggaaa ccctgcgtca ggtcacccaa catgccgagc acgttgttaa tgcgctgaat
    81541 acggaagcga aactgccctg caaactggtg ttgaaaccgc tgggcaccac gccggatgaa
    81601 atcaccgcta tttgccgcga cgcgaattac aacgatcgtt gcgctggtct ggtggtgtgg
    81661 ctgcacacct tctccccggc caaaatgtgg atcaacggcc tgaccatgct caacaaaccg
    81721 ttgctgcaat tccacaccca gttcaacgcg gcgctgccgt gggacagtat cgatatggac
    81781 tttatgaacc tgaaccagac tgcacatggc ggtcgcgagt tcggcttcat tggcgcgcgt
    81841 atgcgtcagc aacatgccgt cgttaccggt cactggcagg ataaacaagc ccatgagcgt
    81901 atcggctcct ggatgcgtca ggcggtctct aaacaggata cccgtcatct gaaagtctgc
    81961 cgatttggcg ataacatgcg tgaagtggcg gtcaccgatg gcgataaagt tgccgcacag
    82021 atcaagttcg gtttctccgt caatacctgg gcggttggcg atctggtgca ggtggtgaac
    82081 tccatcagcg acggcgatgt taacgcgctg gtcgatgagt acgaaagctg ctacaccatg
    82141 acgcctgcca cacaaatcca cggcgaaaaa cgacagaacg tgctggaagc ggcgcgtatt
    82201 gagctgggga tgaagcgttt cctggaacaa ggtggcttcc acgcgttcac caccaccttt
    82261 gaagatttgc acggtctgaa acagcttcca ggtctgcccg tacagcgtct gatgcagcag
    82321 ggttacggct ttgcgggcga aggcgactgg aaaaccgccg ccctgcttcg catcatgaag
    82381 gtgatgtcaa ccggtctgca gggcggcacc tcctttatgg aggactacac ctatcacttc
    82441 gagaaaggta atgacctggt gctcggctcc catatgctgg aagtctgccc gtcgattgcc
    82501 gtagaagaga aaccgatcct cgacgttcag catctcggta ttggtggtaa ggacgatcct
    82561 gcccgactga tcttcaatac ccaaaccggt ccagcgattg tcgccagcct gattgatctc
    82621 ggcgatcgtt accgtctgct ggttaactgt atcgacacgg tgaaaacacc gcactccctg
    82681 ccgaaactgc cggtggcgaa tgcactgtgg aaagcgcaac cggatctgcc aactgcttcc
    82741 gaagcgtgga tcctcgctgg tggcgcgcac cataccgtct tcagccatgc gctgaacctc
    82801 aacgatatgc gccagttcgc cgagatgcac gacattgaaa tcacggtgat tgataacgac
    82861 acccgcctgc cagcgtttaa agacgcactg cgctggaacg aagtgtatta cggatttcgt
    82921 cgctaagtag ccacatcagg tatgtaacgc ctgatgcgac gctgacgcgt cttatctggc
    82981 ctacacgctg cgattttgta ggccggataa gcaaagcgca tccggcattc aacgcctgat
    83041 gcgacgctgg cgcgtcttat caggcctacg cgctgcgatt ttgtagaccg gataagcgaa
    83101 gcgcatccgg cacgaaggag tcaacatgtt agaagatctc aaacgccagg tattagaagc
    83161 caacctggcg ctgccaaaac acaacctggt cacgctcaca tggggcaacg tcagcgccgt
    83221 tgatcgcgag cgcggcgtct ttgtgatcaa accttccggc gtcgattaca gcgtcatgac
    83281 cgctgacgat atggtcgtgg ttagcatcga aaccggtgaa gtggttgaag gtacgaaaaa
    83341 gccctcctcc gacacgccaa ctcaccggct gctctatcag gcattcccct ccattggcgg
    83401 cattgtgcat acgcactcgc gccacgccac catctgggcg caggcgggtc agtcgattcc
    83461 agcaaccggc accacccacg ccgactattt ctacggcacc attccctgca cccgcaaaat
    83521 gaccgacgca gaaatcaacg gcgaatatga gtgggaaacc ggtaacgtca tcgtagaaac
    83581 ctttgaaaaa cagggtatcg atgcagcgca aatgcccggc gttctggtcc attcccacgg
    83641 cccgtttgca tggggcaaaa atgccgaaga tgcggtgcat aacgccatcg tgctggaaga
    83701 ggtcgcttat atggggatat tctgccgtca gttagcgccg cagttaccgg atatgcagca
    83761 aacgctgctg gataaacact atctgcgtaa gcatggcgcg aaggcatatt atgggcagta
    83821 atgactgtat aaaaccacag ccaatcaaac gaaactaggc tatactcaag cctggttttt
    83881 tgatggattt tcagcgtggc gcaggcaggt tttatcttaa cccgacactg gcgggacacc
    83941 ccgcaaggga cagaagtctc cttctggctg gcgacggaca acgggccgtt gcaggttacg
    84001 cttgcaccgc aagagtccgt ggcgtttatt cccgccgatc aggttccccg cgctcagcat
    84061 attttgcagg gtgaacaagg ctttcgtctg acaccgctgg cgttaaagga ttttcaccgc
    84121 cagccggtgt atggccttta ctgtcgtgcc catcgccaat tgatgaatta cgaaaagcgc
    84181 ctgcgtgaag gtggcgttac cgtctacgag gccgatgtgc gcccgccaga acgctatctg
    84241 atggagcggt ttatcacctc accggtgtgg gtcgagggtg atatgcacaa tggcactatc
    84301 gttaatgccc gtctgaaacc gcatcccgac tatcgtccgc cgctcaagtg ggtttctata
    84361 gatattgaaa ccacccgcca cggtgagctg tactgcatcg gcctggaagg ctgcgggcag
    84421 cgcatcgttt atatgctggg gccggagaat ggcgacgcct cctcgcttga tttcgaactg
    84481 gaatacgtcg ccagccgccc gctgctgctg gaaaaactca acgcctggtt tgcaaaccac
    84541 gatcccgatg tgatcatcgg ctggaacgtg gtgcagttcg atctgcgaat gctgcaaaaa
    84601 catgccgagc gttaccgtct tccgctgcgt cttgggcgcg ataatagcga gctggagtgg
    84661 cgcgagcacg gctttaaaaa cggcgtcttt tttgcccagg ctaaaggtcg gctaattatc
    84721 gacggtatcg aggcgctgaa atccgcgttc tggaatttct cttcattctc gctggaaact
    84781 gtcgctcagg agctattagg cgaaggaaaa tctatcgata acccgtggga tcgaatggac
    84841 gaaattgacc gccgtttcgc cgaagataaa cctgcgctgg caacttataa cctgaaagat
    84901 tgcgagctgg tgacgcagat cttccacaaa actgaaatca tgccattttt actcgaacga
    84961 gcaacggtga acggcctgcc ggtggatcgg cacggcggtt cggtggcagc gtttggtcat
    85021 ctctattttc cgcgaatgca tcgcgctggt tatgtcgcgc ctaatctcgg cgaagtgccg
    85081 ccgcacgcca gccctggcgg ctacgtgatg gattcacggc cagggcttta tgattcagtg
    85141 ctggtgctgg actataaaag cctgtacccg tcgatcatcc gcacctttct gattgatccc
    85201 gtcgggctgg ttgaaggcat ggcgcaacct gatccagagc acagtaccga aggttttctc
    85261 gatgcctggt tctcacggga gaaacattgc ctgccggaga ttgtgactaa catctggcac
    85321 gggcgcgatg aagccaaacg tcagggtaat aagccgcttt cgcaggcgct gaagatcatt
    85381 atgaatgcct tttatggcgt gctcggcacc accgcctgcc gcttcttcga tccgcgcctg
    85441 gcatcgtcga tcaccatgcg tggtcatcag atcatgcggc agaccaaagc attgattgaa
    85501 gcgcagggct acgacgttat ctacggagat accgactcga cttttgtctg gctaaaaggt
    85561 gcacattcgg aagaagaggc ggcgaaaatc ggtcgtgcac tggtgcagca cgttaacgcc
    85621 tggtgggcgg aaacgctgca aaaacaacgg ctgaccagcg cattagaact ggagtatgaa
    85681 actcatttct gccgttttct tatgccaacc attcgcggag ctgataccgg cagtaaaaag
    85741 cgctacgccg ggctgattca ggagggcgat aagcagcgga tggtgtttaa agggctggaa
    85801 accgtgcgca ccgactggac gccgctggcc cagcagtttc agcaggagct gtacctgcgt
    85861 atcttccgca acgagccata tcaggagtac gtccgccaaa ccatcgacaa actgatggcg
    85921 ggtgaactgg atgcgcggct ggtttaccgt aaacgccttc gccgtccgct gagcgaatat
    85981 cagcgtaatg ttccgcctca tgtacgcgcc gctcgccttg ccgatgaaga aaatcaaaag
    86041 cgcggtcgcc ccttgcaata tcagaatcgc ggcaccatta agtacgtatg gaccaccaac
    86101 ggcccggagc cgctggacta ccaacgttca ccactggatt acgaacacta tctgacccgc
    86161 cagctacaac ccgtggcgga gggaatactc ccttttattg aggataattt tgctacactt
    86221 atgaccgggc aacttgggct attttgagca aaaaaaagag ttcgccagat accattttga
    86281 tgcgtgacga atgctttgcc atccagtacc atagcgccct ttccattcct ggacctgaat
    86341 aacaccacta cctcataagc acggtagcgg gtggttattg cctgcaatta aagatataga
    86401 gccgaacaca tatgcctttt acacttggtc aacgctggat cagcgataca gaaagcgaat
    86461 tgggacttgg aaccgttgtc gcggtggatg cgcgaactgt cactttactt ttcccatcta
    86521 ctggtgaaaa ccgtctgtac gcacgcagtg attcccccgt gacccgcgtg atgttcaacc
    86581 ctggtgatac cattaccagc catgacggct ggcagatgca agtcgaagaa gtaaaagaag
    86641 aaaatggctt gctgacctat atcggtactc gcctggatac tgaagagtcc ggcgtagccc
    86701 tgcgtgaagt tttccttgat agcaaactgg tgttcagcaa accgcaggac cgtctgtttg
    86761 ccgggcagat tgaccgtatg gaccgctttg cgctgcgtta tcgcgcgcgt aagtattcca
    86821 gcgaacagtt ccgtatgccg tacagcggcc tgcgcggtca gcgtaccagc ctgatcccgc
    86881 atcagctcaa catcgctcat gatgttggtc gccgccacgc gccgcgcgtc ctgctggctg
    86941 acgaagtggg tttagggaaa accattgaag ccgggatgat cctgcatcag caactgctct
    87001 ctggcgctgc tgaacgtgtg ctgattatcg tcccggaaac cttacagcat cagtggctgg
    87061 tagaaatgct gcgccgtttc aacctgcgct ttgctctgtt tgatgatgag cgttatgccg
    87121 aagctcagca cgatgcttac aacccgtttg acaccgaaca gctggtgatt tgctcgctgg
    87181 attttgcccg tcgtagcaaa cagcgcctgg aacatctctg tgaagccgaa tgggacctgc
    87241 tggtggtcga tgaagcgcat cacctggtgt ggagcgaaga tgcgccaagc cgtgaatatc
    87301 aggccattga acaactggca gagcacgtgc cgggcgttct gctgctgacc gcgaccccgg
    87361 aacagctggg gatggaaagc cacttcgccc gtctgcgtct gctggacccg aaccgtttcc
    87421 acgattttgc gcagttcgtt gaagagcaga aaaattatcg tccggttgcg gacgccgttg
    87481 ccatgctgct ggcaggtaac aaactgagca atgacgaact gaacatgctc ggcgagatga
    87541 tcggcgagca ggatatcgag ccgctgttgc aggcagcaaa cagcgacagc gaagatgccc
    87601 agagcgcccg tcaggagctg gtttcgatgc tgatggatcg ccacggcacc agccgcgtgc
    87661 tgttccgtaa cacgcgtaac ggtgtgaaag gattcccgaa acgcgagctg cacaccatta
    87721 agctgccgct accgacgcag tatcagacgg ctattaaagt ctccggcatt atgggcgcac
    87781 gtaaaagtgc ggaagatcgt gctcgcgata tgctctaccc ggagcgtatt tatcaggaat
    87841 ttgaaggtga taacgccacc tggtggaact tcgatccgcg cgttgagtgg ctgatgggct
    87901 acctgaccag ccatcgctct cagaaagtgc tggtgatctg cgccaaagct gccactgcgc
    87961 tgcaactgga gcaggtactg cgcgaacgtg aaggtattcg cgctgcggtg ttccacgaag
    88021 gtatgtcgat tatcgaacgt gaccgcgctg ccgcctggtt tgccgaagaa gacaccggcg
    88081 cacaggtact gctgtgctca gaaatcggtt ctgaaggacg taactttcag ttcgccagcc
    88141 acatggtgat gtttgacctg ccattcaacc cggatctact ggagcagcgt attggtcgtc
    88201 tggatcgtat cggccaggcg cacgatattc agatccatgt gccttatctg gagaaaaccg
    88261 ctcagtcggt gctggtgcgc tggtatcacg aaggtctgga tgcatttgag cacacctgcc
    88321 cgaccggacg cactatttac gatagcgtat acaacgatct gattaactat ctggcttcac
    88381 cggatcaaac cgaaggcttt gacgatctga tcaaaaactg ccgcgagcaa catgaagcgc
    88441 tgaaagcaca gctggaacag ggtcgtgacc gcctgctgga aatccactcc aacggtggcg
    88501 aaaaagccca ggcactggca gaaagcattg aagagcagga tgacgatacc aacctgatcg
    88561 ccttcgccat gaacctgttc gatattatcg gtatcaatca ggacgatcgc ggcgacaaca
    88621 tgatcgtgct gacgccgtcc gatcatatgc tggtgccgga cttccctggc ctgtcggaag
    88681 atggcatcac catcaccttt gatcgtgaag tggcgctggc gcgtgaagat gcacagttta
    88741 ttacctggga gcatccgctg atccgcaacg gtctggatct gatcctttct ggcgataccg
    88801 gtagcagcac gatttcactg ttaaaaaaca aagcgttgcc agtaggtacg ctgttggtgg
    88861 aactgattta tgtggttgaa gcccaggctc cgaagcagtt gcagctcaac cgcttcctgc
    88921 caccgacgcc ggtacgtatg ctgctggata aaaacggcaa caacctggcg gcgcaggtag
    88981 agtttgaaac ctttaaccgc cagcttaacg cggttaaccg tcacaccggc agcaaactgg
    89041 ttaacgccgt gcagcaggat gttcacgcta tccttcaact gggtgaagcg cagatcgaga
    89101 aatctgcccg tgcattgatt gatgcagcgc gtaacgaagc cgacgaaaaa ctgtctgccg
    89161 agctgtctcg tctggaagct ctgcgtgcag tgaacccgaa cattcgtgac gacgaactga
    89221 ccgccattga gagcaaccgt cagcaggtaa tggaaagcct ggatcaggca ggttggcgtc
    89281 tggatgccct gcgtttgatc gttgtaacgc atcagtaacg gagtcgaaaa tggggatgga
    89341 aaactacaat ccaccgcagg aaccctggtt ggttatcctg tatcaggatg accatattat
    89401 ggtggtcaac aagccgagcg gtttgttgtc agtgccgggt cgtctggaag agcacaaaga
    89461 cagcgtgatg acgcgcattc agcgtgatta tccgcaggca gaatcggtgc atcgtctgga
    89521 tatggctacc agcggcgtga ttgtagtggc gctgaccaaa gccgcggagc gggagttaaa
    89581 acgccagttc cgcgagcgcg agccgaaaaa gcagtatgtg gcccgcgtct gggggcatcc
    89641 atcccccgca gaaggtctgg tggatctgcc gctgatttgc gactggccaa accgcccgaa
    89701 acagaaagtc tgttacgaaa cgggtaaacc tgcgcagacg gaatatgaag tggtggagta
    89761 tgcggcggat aacacggcaa gagtggtgtt aaaaccgatt accgggcgtt cgcatcaatt
    89821 gcgtgtgcat atgctggcgc tggggcatcc gattctcggc gatcgttttt atgcatcacc
    89881 agaagcgaga gcgatggcac cgcgtttgtt gctacatgca gagatgttga cgattaccca
    89941 tccggcgtat ggcaatagta tgacgtttaa agcgccagcg gatttttaat cgtcacgctg
    90001 gcgcagttcg gtaggcggat aaggcgttca cgccgcatcc ggcaattgtg cgccgattgc
    90061 ctgatgcgac gcgggccgcg tcttatcagg cctacagttt aaaacttatt taaacccttt
    90121 ctgctgcttt atcagctcat atgcctgctg aatttcctgc gctttctgct tcgccatctc
    90181 catcatctca ggcggcaaac ctttcgccac cagcttatcg ggatggtgtt cactcatcag
    90241 cttacggtag gcacgtttga tggtggtcgc atcatccgtc ggcttcacgc ccagcacatt
    90301 acaggcatct tccagcgtcg ggccacgctg cgcttgctgc cagttaccac cgccagattg
    90361 ctgctgataa ccgccgccaa actgtgcacc gccctgcatc atgcgcaaaa actggtcaaa
    90421 ctgggcgcgg gagatcccta attcttcagc aatgacatac agcaccgccc gttcattcgg
    90481 gtgcagtgaa ccatcagcaa acgccgcctg aatctggatc tccagaaaca tacgaattaa
    90541 gtcaaaacga ccaaagcaga cactgcgaaa ctggcgcatc ttttcgcgca gcgggtaatt
    90601 atctgatttt cccacccgga acgcgttttg cgccgcagta cgggaagcgc catgaaggtt
    90661 cattcggtcc atcaactggc tggcgatatg aatatcagcc tccgtgacgc gacctttgga
    90721 tttggttaaa tgccccatca cttcaaaagt ggtggcaaaa aacagcgcct gacgctcacg
    90781 ctggttggcg aaccacgcca ttttacggct acgggcttta tcaaacatat ggccaattaa
    90841 caggcctaac actacgcccc aaaagccgcc gcccatcagt aaggccacgg ccacgccaat
    90901 gatttttccc caatactgca tatattcccc aaatcgacac acggatatca gggctatctc
    90961 ccacaatata aaggtgcttt taccgttttc cggctgcggt caattgtggg acatcgccta
    91021 taatttgcat tatcatacct gtcattcaat gccgtgccta acaccactga cgctattcgg
    91081 acaggattaa cactagcgta gagatgacga gtacgttagt ctctgaccgt ttgtcacgcg
    91141 caacgttacc gatgatggaa caataaaatc aacgtatgaa aaaacgtatc cccactctcc
    91201 tggccaccat gattgccacc gccctttata gtcaacaggg actggcagcc gacctcgcct
    91261 cacagtgcat gttgggcgtg ccaagctatg accgtcctct ggtacagggc gataccaatg
    91321 acttacccgt gactatcaat gctgaccacg cgaaagggga ctacccggat gacgccgtgt
    91381 ttactggcag cgtggatatc atgcagggta acagccgtct gcaggccgac gaagtgcagc
    91441 tccatcaaaa agaggcacta ggacaaccgg agccggtacg taccgttgat gcgctcggta
    91501 atgtccatta cgacgataac caggtgatcc tcaaagggcc gaaaggctgg gcgaatctga
    91561 acaccaaaga taccaacgtc tgggaaggtg attaccagat ggtgggtcgc cagggtcgcg
    91621 gtaaagcgga cctgatgaaa caacgtggcg aaaaccgcta taccattctg gataacggta
    91681 gctttacctc ctgtctgccg ggttctgaca cctggagcgt ggtaggtagc gaaattattc
    91741 atgaccgcga agaacaagtt gcggagatct ggaacgcccg ctttaaggtg ggtccggtac
    91801 cgatctttta tagcccctat ttgcagttgc cggtgggtga caaacgtcgc tctggtttct
    91861 tgatcccgaa cgccaagtac accaccacca actactttga gttctacctg ccatattact
    91921 ggaacatcgc gccaaatatg gatgccacca tcacgccgca ttatatgcat cgtcgtggca
    91981 acatcatgtg ggagaacgaa ttccgctacc tctcccaggc tggcgctggc ttgatggaac
    92041 tggactatct gccttcagat aaagtctatg aagatgaaca cccgaacgat gacagttcac
    92101 gtcgttggtt attctactgg aaccactccg gggtcatgga tcaggtgtgg cgtttcaacg
    92161 tcgactacac caaggtcagc gatcctagct acttcaatga tttcgataac aagtacggtt
    92221 ccagtactga cggctacgca acgcaaaaat tcagcgttgg ctatgcggtg caaaacttca
    92281 atgccaccgt ttcaaccaag cagttccagg ttttcagcga acagaacacc agtagctact
    92341 cggcagagcc gcagttagac gttaattact accagaatga tgttggtccg tttgatacgc
    92401 gtatttacgg ccaggcagtg cactttgtta acaccagaga cgacatgcct gaagcaaccc
    92461 gtgttcacct agaaccgacc atcaatttgc ctctctctaa taactggggc agcatcaata
    92521 ccgaagcgaa gtttctggca acccattatc agcaaaccaa tcttgactgg tataactcca
    92581 gaaacacgac caagctggac gaatccgtta accgcgtaat gccgcaattc aaagttgacg
    92641 gcaaaatggt ctttgaacgc gatatggaaa tgctggctcc gggttatacc caaacgctgg
    92701 aaccgcgcgc gcagtatttg tacgtgccgt atcgcgatca gagcgacatc tataactacg
    92761 actcgtctct gctgcaatct gactactctg gcctgttccg ggaccggact tacggcggtc
    92821 ttgaccgtat tgcctccgct aaccaggtga cgaccggtgt cacatctcgc atatatgatg
    92881 atgctgccgt tgaacgtttt aatatttccg ttggtcaaat ctactatttc acggagtctc
    92941 gcactggcga tgacaacata acatgggaga atgacgacaa aacgggctca ctggtgtggg
    93001 caggcgacac ctactggcgt atctccgagc gttggggatt gcgtggcggg attcagtacg
    93061 atacacgtct ggataacgta gcgaccagta actccagcat tgaataccgt cgggatgaag
    93121 accgtctggt acagctgaat taccattacg ccagcccgga atatattcag gctacgctgc
    93181 ctaagtacta ttccactgct gagcaatata agaatggtat ttcgcaggta ggtgctgtcg
    93241 ccagctggcc aattgccgat cgttggtcca ttgttggggc ctactactac gacaccaatg
    93301 cgaacaagca agccgactct atgttaggtg tgcaatacag ctcctgctgc tatgcaattc
    93361 gcgtcggtta cgagcggaag ctgaacggtt gggataacga taaacaacat gcggtatatg
    93421 acaacgcaat cggctttaac atcgaacttc gcggcctgag ctccaactac ggtctgggta
    93481 cgcaagagat gctgcgttcg aacattctgc cgtatcaaaa cactttgtga tttgttgatt
    93541 taccacgtaa tccgcagtgc ggttaattga aatggaaaaa gtatgaagaa ctggaaaacg
    93601 ctgcttctcg gtatcgccat gatcgcgaat accagtttcg ctgcccccca ggtagtcgat
    93661 aaagtcgcag ccgtcgtcaa taacggcgtc gtgctggaaa gcgacgttga tggattaatg
    93721 cagtcggtaa aactgaacgc tgcgcaggca aggcagcagc ttcctgatga cacgacgctg
    93781 cgccaccaaa tcatggaacg tttgatcatg gatcaaatca tcctgcagat ggggcagaaa
    93841 atgggagtga aaatctccga tgagcagctg gatcaggcga ttgctaacat cgcgaaacag
    93901 aacaacatga cgctggatca gatgcgcggc cgtctggctt acgatggtct gaactacaac
    93961 acttatcgta accagatccg caaagagatg attatctctg aagtgcgtaa caacgaggtg
    94021 cgtcgtcgca tcaccatcct gccgcaggaa gtcgaatccc tggcgcagca ggtgggtaac
    94081 caaaacgacg ccagcactga gctgaacctg agccacatcc tgatcccgct gccggaaaac
    94141 ccgacctctg atcagttgaa cgaagcggaa agccaggcgc gcgccattgt cgatcaggcg
    94201 cgtaacggcg ctgatttcgg taagctggcg attgctcatt ctgccgacca gcaggcgctg
    94261 aacggcggcc agatggggtg gggccgtatt caggagttgc cggggatctt cgcccaggca
    94321 ttaagcaccg cgaagaaagg cgacattgtt ggcccgattc gttccggcgt tggcttccat
    94381 attctgaaag ttaacgacct gcgcggcgaa agcaaaaata tctcggtgac cgaagttcat
    94441 gctcgccata ttctgctgaa accgtcgccg atcatgactg acgaacaggc ccgcgtgaaa
    94501 ctggagcaaa ttgctgctga tatcaagagt ggtaaaacga cttttgctgc cgcagcgaaa
    94561 gagttctcgc aggatccagg ttctgctaac cagggcggtg atctcggctg ggctacacca
    94621 gatattttcg atccagcctt ccgtgacgcc ctgacccgcc tgaacaaagg tcaaatgagt
    94681 gcaccggttc actcttcatt cggctggcat ttaatcgaac tgctggatac ccgtaatgtc
    94741 gataaaaccg acgctgcgca gaaagatcgt gcataccgca tgctgatgaa ccgtaagttc
    94801 tcggaagaag cagcaagctg gatgcaggaa caacgtgcca gcgcctacgt taaaatcctg
    94861 agcaactaat ggttaaaacc caacgtgttg tgatcactcc cggcgagccc gccgggattg
    94921 gcccggactt agttgtccag cttgcacagc gtgagtggcc ggtcgaactg gttgtttgtg
    94981 ccgatgccac tctccttacc gaccgggcag cgatgctcgg tttgccgctc accctccgcc
    95041 cttattcccc caactcccct gcacaaccgc aaactacggg cacattaacg ctacttcctg
    95101 tcgcgctacg tgaatctgtc actgcggggc agttagcgat tgaaaatggg cattacgtgg
    95161 tggagacgct ggcgcgagcg tgtgatggct gtctgaacgg tgaatttgct gcgctgatca
    95221 caggccccgt gcataaaggc gtcattaacg acgcaagcat tccgtttacc ggtcataccg
    95281 agtttttcga agagcgttcg caggcgaaaa aagtggtgat gatgctggcg accgaagaac
    95341 ttcgcgtggc gctggcaacg acgcatttac cgctgcgcga tatcgcagat gctatcaccc
    95401 ctgcactttt gcacgaagtg attgctattt tgcatcacga tttgcgtaca aaatttggta
    95461 ttgccgaacc gcgcattctg gtctgcgggc tgaatccgca cgcgggcgaa ggcggtcata
    95521 tgggtacgga agagatagac accattattc cggtgctcga cgagctgcgg gcgcagggga
    95581 tgaaactcaa cgggccgctg cctgccgata ccctgtttca gccgaaatat ctcgataacg
    95641 ccgacgccgt gctggcgatg taccacgatc agggtcttcc cgtgctaaaa taccagggct
    95701 tcgggcgcgg tgtgaacatt acgctgggcc tgccctttat tcgcacatca gtggaccacg
    95761 gcaccgcgct tgaactggcg ggacgtggcg aagccgatgt cggcagtttt attacggcgc
    95821 ttaatctcgc catcaaaatg attgttaaca cccaatgaat aatcgagtcc accagggcca
    95881 cttagcccgt aaacgcttcg ggcaaaactt tctcaacgat cacttcgtga tcgacagcat
    95941 tgtttctgcc attaacccgc aaaagggcca ggcaatggtc gaaatcggcc ccggtctggc
    96001 ggcattgacc gagccggtcg gcgaacgtct ggaccagctg acggtcatcg aacttgaccg
    96061 cgatctggcg gcacgtctgc aaacgcatcc attcttaggc ccgaaactga cgatttatca
    96121 gcaggatgcg atgaccttta actttggcga actggccgag aaaatgggtc agccgctgcg
    96181 tgttttcggc aacctgcctt ataacatctc cacgccgttg atgttccatc tgtttagcta
    96241 tactgatgcc attgccgaca tgcactttat gttgcaaaaa gaggtggtga atcgtctggt
    96301 tgcaggaccg aacagcaaag cgtatggtcg attaagcgtc atggcgcaat actattgcaa
    96361 cgtgatcccg gtactggaag ttccgccgtc agcctttaca ccaccaccca aagtggattc
    96421 cgccgtcgtg cgcctagtcc ctcatgcaac gatgcctcac ccggttaaag atgttcgcgt
    96481 gttgagccgc atcaccaccg aagcctttaa ccagcgtcgt aaaaccattc gtaacagcct
    96541 cggcaacctg tttagcgtcg aggtgttaac cggaatgggg atcgacccgg cgatgcgagc
    96601 ggaaaatatc tctgtcgcgc aatattgcca gatggcgaac tatctggcgg agaacgcgcc
    96661 tttgcaggag agttaacgat gatcaattcg ccccgagtgt gtattcaggt tcaaagcgtc
    96721 tacattgagg cacaatcttc acctgataat gaacgttacg tttttgctta taccgtaacc
    96781 atacgcaatc tggggcgagc gccagtgcag ttgttggggc gttactggct gatcaccaat
    96841 ggcaatggcc gtgaaaccga agtccatggc gaaggagtgg ttggcgtcca gccacttatc
    96901 gcgcctggcg aagagtacca gtacaccagc ggtgcaatca ttgaaacccc gctgggcacc
    96961 atgcagggtc actacgaaat gatcgatgaa aatggcgtcc ctttcagcat cgacattccc
    97021 gtattccgac tcgccgttcc cacactcatt cattaaaaga atatggcgac ataccttatt
    97081 ggcgacgttc atggttgtta cgatgaactg atcgcattgc tgcataaagt agaatttacc
    97141 cccgggaaag ataccctctg gctgacgggc gatctggtcg cgcgcggccc gggttcgctg
    97201 gatgttctgc gctatgtgaa atccttaggc gacagcgtac gtctggtgct gggcaatcac
    97261 gatctgcatc tgctggcggt atttgccggg atcagccgca ataaaccgaa agatcgcctg
    97321 acaccgctgc tggaagcgcc ggatgccgac gagctgctta actggctgcg tcgccagcct
    97381 ctgctgcaaa tcgacgaaga gaaaaagctg gtgatggccc acgcagggat cacgccgcag
    97441 tgggatctgc agaccgccaa agagtgcgcg cgcgatgtag aagcggtgct gtcgagtgac
    97501 tcctatccct tctttcttga cgctatgtac ggcgatatgc caaataactg gtcaccggaa
    97561 ttgcgggggc tgggaagact gcgttttatc accaacgcct ttacccgtat gcgtttttgc
    97621 ttcccgaacg gtcaactgga tatgtacagc aaagaatcgc cggaagaagc ccctgcccca
    97681 ctgaaaccgt ggtttgcgat tcctggccct gtcgctgaag aatacagcat cgcctttggt
    97741 cactgggcat cgctggaagg caaaggtacg ccggaaggta tttacgcgct ggataccggc
    97801 tgctgctggg gtgggtcatt aacctgcctg cgctgggaag ataaacagta ttttgtccag
    97861 ccgtcgaacc ggcataagga tttgggcgaa gcggcggcgt cttaaacaca gcctgataca
    97921 ggaaggccgg ataagacgcg accggcgtcg catccggcac tagccgtaaa ttctatacaa
    97981 acttaccgcc gctccagaat ctcaaagcaa tagctgtgag agttctgcgc atcggcgtcg
    98041 tggaattcgc tgaataccga ttcccagtca tccggctcgt aatccgggaa atgggtgtcg
    98101 ccttccactt ctgcgtcgat atgcgtcaga tacagttttt gcgcttttgg caggaactgc
    98161 tcataaacgc gaccgccgcc aatcaccatg atttctggta cgtcaccaca cgccgcaatg
    98221 gcttcatcca ccgatttcac ccacgttacg cgatcgtccg tacccggttg actgctgagg
    98281 ataatatttt tgcgtcctgg caacggacga ccgattgatt cccaagtatg gcggcccata
    98341 atcacgggtt tatttaaggt gttgcgttta aaccaggcga gatcggcagg caggttccac
    98401 ggcatggcgt tttccatgcc gataacgcga tctaccgcta acgccgcaat cagactgatc
    98461 attgagattt cccgataaaa aaattgtcgc cactatacgt aaagcgtaaa ccgtcgtcga
    98521 ctggtgcgag gatgatgttg aggaaaattt tatattctgc tggcgagtcc acgctctccc
    98581 cctggactcg ccgcattaca atgaaacaaa aacaaacagt tagctgtaaa gtgtgattta
    98641 cgtcactctt tattaagatg agggtttcgt ttccggttca tccgccatgt tgccggtatg
    98701 tttaccttct tctgttccct gccagccatg tcgttgaatt aatgacagat gttcgcggtc
    98761 ctcggtgatg atctcactta acatcgcgct ggtgcgttta tagaccgccg cgcgggcttt
    98821 ggtgtcattc tcaaccattg ccatctcttc caccatctga atattaaagc ggcggaacac
    98881 atcggcacgt tctcgcgctt catacggccc cagacctaaa ctttccagcg ccagacgccc
    98941 ggttttcagc gcaccttcaa aggtttcacg ctccggcttt tcaaggcctg cctgacgcaa
    99001 acgaatgtag tgatcgacat cgcgggcgcg ggcaataatc tgcaaatgcg ggaaatgttc
    99061 tttcaccatc tccgtcagtt gcaggttggt ttgcggatcg tcaatagcgt taatcagcac
    99121 ttccgctttc gccgctccgg cagattccag taaatccatc cgcgtggcat cgccataaaa
    99181 cactttcata ccaaatttac gcagcgtttc gatatggtcc ggatcgtgat cgagcaccac
    99241 cattttcacc ccgctggaga gcagtaaacg tccggtaatc tgtccaaaac gaccgaatcc
    99301 ggcgataatc acgcgcggct gttcttcatc gatctcatcg gcttcacgcg cctcctccgt
    99361 agaagattgc tcaaggcgat tgaggatcac cagcagaatc ggcgttgctg ccatcgacag
    99421 cgccaccgcc agggtcagcg atttcgccca ctccggctcc agcacattcg ccatctgcgc
    99481 cgcaccaaat accacaaagg caaactcact gccctgccct aacaacaccg caaaccagcg
    99541 acgctgttta tttggcacct gcaacggtcg ggcaatcagc cacagcatgg cgattttgat
    99601 gatgaggaaa ccgagcagca aaatgacaat gcgcaatggg ttttcaatca gcgtgccaaa
    99661 gtctatcgac atgccaacac cgatgaaaaa cagccccaac agcaaacctt taaacggttc
    99721 gatatcgctt tccaacgcat gacggtattc cgagcttgcc agcagtacgc ccgccagaaa
    99781 cgcgcccatc gccatcgaca agccgacctc ttccagcagc aaaccaaagc caaacacaag
    99841 gaataacgcc acgacactaa acacttcccg caagccagag cgggcaacaa aacgcagcgc
    99901 cggacgcgta acatagcgcc ccagcaatac caccagtacc agcgcacccg ccacttttaa
    99961 cgccgagaga gcaaatgcgc ccatcgtcgt cgaggcactg ctcgccgcca gtagcgggat
   100021 catcgccacc agcgggattg ccgcgatatc ctggaacagc agcaccgcaa aggcactgcg
   100081 gcccatttgc gtcaccatca gattgcgttc attcatcgcc tgcatggcaa tcgccgttga
   100141 tgagagcgcc agcgtcatac cgatcaattc cgcgacctgc cagcgcagcc caagtaacat
   100201 acagaacagc cccagcaggc caccgcaaat caccatctgc aatgcgccac cgccgaacac
   100261 tgccgcacgc agcttccaca gcctttgtgg atcgagttcg aggcgataat aaacagcatc
   100321 agcaccaccc caatctcggc aaagtgcaga atagattcgg catcggtcac cagtcgcagc
   100381 ccccacgggc caataatgca gccggcgatc aggtagccaa gtaccgatcc cagaccaaga
   100441 cgtaccgcaa tgggtacaat cagcgccgcc gatccgaggt agatcagcgc ctgaaccagc
   100501 gtatgactat ccatgatggg cctcctgcca ttccagcaga cgttgcttat agtggcgcgc
   100561 ctgcccttcg agggtttcgt cgtcacaaat aaaggtgcag tgcatggcaa acggtggcag
   100621 ccagttcagc ccgcagtaga ttgccgtcgc ctgtagcggc tgcgacagca catcaaagcc
   100681 cggatgcgca ccaatttcaa aatggctttc cccgccgccg gtcgtcaccg cccacagcaa
   100741 atgtttgcca tgcagcgccg tgccgccgtg accgtaagcc cagccgtgcg acaaaacttt
   100801 atcgatccaa agtttaagaa gcggaggaat gctgtaccac tgcatcgggt gctgccagac
   100861 gatcagatcg gcgcgagaga gcgcctcctg ctcggcggca atatcgatat tgaagtcagg
   100921 atagagttga taaagagagc gaatttcgac gccttccagc gtccttgcct gttcaagcat
   100981 ccgtttattc gcatgggaat gatgcggata cggatgcgca taaatgatga ggatcatagt
   101041 tattagcccg cgtgtttacg cctttgtttt cccacaaagt gtagtcatat attcacgcgg
   101101 cttatagtca ataatattga ttcagaaaag agagaaatct gaacaaataa tcccattctc
   101161 tgcccgcgga tcgtcatatt gctggtctgc gccagtgaca ttcctcgagt ctccggggca
   101221 aacgctacgg aaatcaacaa gccaaacagc gagatacccg cccccattag catcgtgtta
   101281 ctgataccgt aattattgat aaatatcggc agtgcccagg tcgaaacaat ggtgccaata
   101341 cgacttaagg acataatcac gcccacggca gaggcgcgga tatccgtcgg gaagagttca
   101401 ttaggataga gccactgcaa attacccggc ccgccagaga aaaaggcata caccgcaaag
   101461 gccatcacta ccagccagat ccccatatcc gggattagcc ccaaaaccgc cagcgccagc
   101521 gtcatcatgg caaagctgcc aatcaacaat ggacgccgtc cggcagtgtt tagccacagc
   101581 atcggcggaa tacagccgag cataaagaac aggctaatca ccacattccc tagtgccgcg
   101641 tttttgccaa cccccaatcc caacgaacca acgatttgcg ggccaaaggt gtaaatggcg
   101701 aacattggga tcacctggca ggtccagatg gcggcaacaa acagaacaaa aggaaaatgg
   101761 cggcgattaa acagatcgcg aaaacgggtt tgctgcggct gctcttcatc gaaagccacc
   101821 ggttcgccaa acagttttat catcatctct tcgcactctt ttactcgccc tttgcgtaat
   101881 aaccagcggg gagattctgg cagttcgaat cgaccaatca aaatcaacaa acaagggatc
   101941 gccgcgctac ccagcatcca gcgccaacca ccttccacat cataaagcca gtagccgacc
   102001 agatcggcgc aggtcgcgcc gacataccac atcgcggcaa taaagctgat ggaaaacgcc
   102061 cgctgacggg tactggagaa ctcggtgatc atcgaagtgg cgatgggata atctgcaccg
   102121 atgacaatgc cgatgagtac ccgcatcacc aacagttcga cgggggatga aacacacatc
   102181 gtcgccaccg atatcacgcc gatggcgatg atatcaatga ggaacatttt gcgccgtccg
   102241 actttatcgg agatatagcc aaacagcgat gtgccaacga acagcccggc gagcgttccc
   102301 gcgcccagca agccaatcca gtcagcgtcc agtttcagcg ccggagtcag ttgctccagc
   102361 gccacgccaa tcattaccag tacataacca tccagaaacg gaccaccgct tccccacagc
   102421 aaaatgcggc ggtgaataga ggagaatttg agatcgtcaa agtttctgga cggttgcatg
   102481 ggttattcct gtttttttgc aatgagagtc agtacggcgt tacgtatcaa accaacatca
   102541 gccgtaacgg aactccacac caaaggtgcc gcgcgggtat tcccactgtt ccagcgccga
   102601 ccccagcccc aaaatgcgac aggtgccgca ctccagacat ccggcgtaat cgaagcgcac
   102661 actgccgtca tcctgcttct tgtacagacc tgcggggcac gctttcacca gcagctccag
   102721 cacctgttta tcagcatcgg ccttcacaac aatgtgcgga tgctcttcat cgacattgaa
   102781 tttattgacg cccagtttga cgtccacatt gacgggagaa gtcataaaac ggtcactcct
   102841 ttcatgccat ccttgatcag attgatgaag cccactttct tgccgtggcg gaggattttc
   102901 ttgcgcatca gttccggcgc actgccgtca atggtgaaca ggtcacgcgc cacgcccacc
   102961 gccagttccg ggtagccgct aaacatgcgt gggttatcaa ggaacgccgg tagtttctgg
   103021 tacatgcgca tatcgcgcag cgggccactc tccagatgct gacgatattc cgcgagtttt
   103081 tgcttactga aatcgtcgct tttcatcgct gaaagcacgg tttttgctgc agcttccccg
   103141 gcggcaatcg ccagatccat accgcgaatg gtaaaaccga ggttcataca cattccggcg
   103201 gcatcaccgg caatcaatac gccgtcaccg accagctccg gcagcatgtt gatgcctgct
   103261 tccggcacta cgtgagcgga atattccacc agcttgccgc ccgcgatcag cggtgcaacg
   103321 gccggatgct gtttgaaatc ttccagcatt tgcggcaccg attttttcgc gtcatgcaga
   103381 tgatgcagac cacaaaccag ccccagcgac agggtatttt cactggtata aaggaagccg
   103441 ccgcccatca ggccatcggt gggtgatccc gcaaacagac aagccgcgcc ctgattaccc
   103501 tgcaactgaa aacggtcttc gattaccgac ttcggtaact cgatcagttc cttcacgcca
   103561 accgccacat ccgtcggttt aacgcgtttt gccatcccca acttttcggc aaggatggag
   103621 ttcaccccat cagcaaggat caccgttttc gcttcaatca catcgccatc ggcttctaca
   103681 ccgacgactt tgccatcgcg ctgtacgagg ttatcgacgc ggatcccggt aattaactgc
   103741 gcgctcgctt cttcggcctg ctccatcaac caggcatcaa atttactgcg caaaacggag
   103801 taagaacgct gggatggcga ggtttcgtca ccgttgcagt agtccatagt catcgctgac
   103861 ttttccgtca taaacgcgag tttttcatgg gtgatcaggc gttctacggg ggcggaatcg
   103921 gcgaaacctg gaataatgtg ttccagactg tgggcataga gacgcccgcc agtaacgttc
   103981 ttggcacctg cggaattgcc acgctcgata actaacactt gcgccccttc gcgggcgagc
   104041 accagtgcgg caaccgaacc ggcaagccct gcaccgacga tgatggcgtc aaagatatct
   104101 tcggacataa gagctccctg tcagcggcag agccgtaatg cagccctgcc agagtggatc
   104161 aacacgctaa agctgcggtc agtgccggaa ggatcttcac ggcatcgcca acaatgccgt
   104221 aatccgcgta ctggaagatc ggcgcatttt tatctttatt gatggcgaaa atggtttgcg
   104281 acgcgttagc gccaaccatg tgctggatct gcccggagat ccccaccgcc aggtacagtt
   104341 caggtttcag catcaggttg gagataccga catagcgttc gtgctccatc catttttcgt
   104401 tttctgccac cggacgagaa caggccaact ccgcacctat cgccttgcaa agctgttctg
   104461 ccagcgcaat gttctctttg ctgccaatgc cgcgaccgac gctgaccacc agacgggctt
   104521 tgtcgagatc gacgctgttg ctctggcgcg cctgggttgc cgtgcgggta atcgccacag
   104581 ccggagcctg ccactccacg gtgtgcgttt cgccagtgcg tgacgcgtct ggctgagccg
   104641 catcgaacgt gccgctgctg atggtcagta ccgcatacgg cgtggcaatg cgttcttcgc
   104701 caatcgccag accgccgtaa accatgtgtt tcactgttgc tttaccgtcc tgtacgctga
   104761 cggtgctggc atcgttagac accgccgctt taaggcgata acccagtttt gccgccagta
   104821 atttgccgcg acgggtgttt ggcaacagca ccagaccgtc agcgccgtgc tggcgaatag
   104881 tgtcagccat gacaccggcg taatcttcga tcatccgatc gtccggtttg ccgtttaatt
   104941 tccagacatg attagcgccg agctggattg cctgtgcgcc gtcggcatca ttgaggacaa
   105001 aggcattgat ttgattagct aaagcctgcg caccgttcat cagttccggc agacgagagg
   105061 gggtatcgct gaatacccag acttgagaaa acgtgttcat agcatcccct gtaattaaat
   105121 gactttgcga agattttcag caaatgcggc gatctgttct tcgccgtcgc cttcaatcac
   105181 gatgcgctga cgctcgcgct gtttcggcgc ggcaacctgt tgttcagacc aggcgtccac
   105241 ggcgttaaaa ccaatatccg ccgccgacca tacctggact ggctttttcg ccgcgccgag
   105301 aatggctttc atcgaaggaa tttgtgggga gttgatatca gtggaaacag caacaaccgc
   105361 aggcagcgga atgcttaagg tttcggtttc atcttccagt tcgcgctcaa cggtgagggt
   105421 atctgccgtc agggagataa ttttgctgac gccgttaact gccggaatat tgaggatttc
   105481 gcccaccagc agaccaacct gctgggcata aaggtcggaa gaaccatcgc cacagaggat
   105541 cagatcaaag cctgctttct gggcggctgc agccagtgcg ctcgccgttt gttgcggcag
   105601 tgcctgctcg aactggtcat caatcaccac aatcagttca tccgggccgc gcgatagcac
   105661 atctttacgc cctttggcgt tggtcagggc tttaccgccc acacttaagg ctgtcacctg
   105721 cgcctctgct gcctgttgct ttagctggca agccgcttca atagcgttga gatcgtattg
   105781 gcttattttg gcatcggctt tgctgaagtc taatgaacca tcagcattat tgaccgcaat
   105841 atcctgttca tcaggcacgc acttatagca agtaataatc ttcattgcat ctccagaaat
   105901 catgaaggaa acgtcgttca tatcggtggt gttaattaca gatatcacgc cctttaattt
   105961 ggaaataaaa ctttaatcac caatattgaa aatgtcacac gcagataaat ccaactttca
   106021 atattgtttt atagatcacc aatattgaaa gtcagatgct atttttttac ccacaaaaga
   106081 acataacaaa ctgattaata accaaaatta acggaaaacg tcctctattc acccgaaaca
   106141 aaaatgtgac aacaatcaca gaatacagct tattgaatac ccattatgag ttagccatta
   106201 acgcgtccac gaggttaata ataattatat taaatgttaa caaaaataaa acaaacggga
   106261 acgcaaaata aatattcgtt ttcacagtgg aattaactca tgaagaatga aaagagaaaa
   106321 acgggaatag aaccgaaggt tttctttccg ccattaataa tcgtcggcat actttgttgg
   106381 cttacagtca gagatctgga tgcggcgaat gtcgttatta atgctgtatt cagttacgtc
   106441 accaatgtat ggggatgggc atttgaatgg tatatggtgg tgatgctttt cggttggttc
   106501 tggctggtgt ttggcccgta tgccaaaaag cgtttaggta acgaaccgcc agaatttagc
   106561 accgccagtt ggatctttat gatgttcgcc tcctgtacgt ctgctgccgt actgttctgg
   106621 ggatcgattg agatctacta ctacatctcc accccgccgt ttggcttaga accgaactcg
   106681 acaggagcga aagagttggg gctggcttac agcttgttcc actggggacc tctgccgtgg
   106741 gccacttaca gcttcctttc agtcgccttc gcttacttct tctttgtccg caaaatggaa
   106801 gtgattcgcc ccagctcgac actggtgccg ctggtaggtg aaaaacacgc caaagggttg
   106861 ttcggtacca tcgtcgacaa cttctatctc gtcgccttga tcttcgcgat gggtaccagt
   106921 ctgggccttg ccacgccgct ggtgaccgag tgtatgcaat ggttgtttgg cattccgcat
   106981 accctgcaac tggacgctat catcattacc tgctggatta tcctcaacgc catttgcgtc
   107041 gcttgcggtc tgcaaaaagg ggtacgtatc gccagtgacg tgcgtagtta cctgagcttc
   107101 ctgatgctgg gttggatgtt catcgtcagc ggtgccagct tcatcatgaa ctacttcacc
   107161 gattcggtgg ggatgttgct gatgtatctg ccgcgcatgt tgttctatac cgatcccatc
   107221 gctaaaggcg gcttcccgca gggctggacc gtgttctact gggcatggtg ggtgatttac
   107281 gccatccaga tgagtatctt cctcgcccgc atctcccgtg gtcgtactgt gcgtgaactg
   107341 tgcttcggca tggtgatggg gctgacagca tccacctgga tcctgtggac cgtgctcggt
   107401 agtaacactc tgctgttgat ggataaaaac atcatcaaca ttccaaatct gatcgaacag
   107461 tacggtgtgg cgcgcgccat catcgaaacc tgggccgctc tgccactcag caccgccacc
   107521 atgtggggct tcttcatcct ctgctttatt gccaccgtta cgctggttaa cgcctgctct
   107581 tataccctgg cgatgtctac ttgccgcgaa gtacgcgatg gtgaagaacc accgctgctg
   107641 gtgcgtatcg gttggtcaat tctggttggc attatcggta ttgttctgct ggcgctcggc
   107701 ggcctgaaac cgattcaaac cgccattatc gccggaggat gcccgctgtt cttcgtcaac
   107761 attatggtga cgctctcctt tattaaagac gcgaaacaga actggaaaga ttaattaacc
   107821 cccaaaatat caagaggttg aaagatggat tttaatttaa atgatgagca ggaactgttt
   107881 gtcgccggta tccgcgaact gatggccagc gaaaactggg aggcctattt tgccgagtgc
   107941 gaccgtgaca gcgtctaccc ggaacgtttt gtcaaagcac tggcggatat gggtatcgac
   108001 agtctgctga tccctgaaga gcacggtggt ctggacgcgg ggtttgttac tctcgccgcc
   108061 gtgtggatgg agctgggacg tctgggggca ccaacctatg tactgtacca gctgccgggc
   108121 gggttcaata ccttcctgcg cgaaggcaca caagagcaga tcgacaagat tatggctttc
   108181 cgcggcaccg gtaagcagat gtggaactca gcgattaccg aaccgggcgc gggctccgac
   108241 gtgggtagcc tgaaaacgac ttatacccgt agaaatggta agatttatct taatggtagt
   108301 aagtgtttta ttaccagtag cgcctacacc ccgtacatcg tggtgatggc gcgcgacggg
   108361 gcttctccgg acaaacctgt ctacaccgaa tggtttgttg atatgagcaa accgggcatc
   108421 aaagtgacca aacttgagaa gctcggtctg cgtatggata gctgctgtga aatcaccttt
   108481 gacgatgtgg aactggacga gaaagacatg ttcggtcggg aaggtaacgg ctttaaccgc
   108541 gtcaaagaag agttcgacca tgaacgtttc ctggtagccc tgaccaacta cggtacggcg
   108601 atgtgcgcct ttgaagatgc tgcgcgctac gccaaccagc gcgtgcagtt tggcgaggct
   108661 attggtcgtt tccagttgat tcaggaaaaa ttcgcccaca tggcgatcaa attaaactcc
   108721 atgaaaaaca tgctgtatga agcagcgtgg aaagcagaca acggcaccat cacctctggc
   108781 gatgcagcga tgtgcaaata cttctgcgcc aatgcggcat ttgaagtggt ggatagcgca
   108841 atgcaggtgc tgggcggtgt cgggattgcg ggcaaccacc gcatcagccg cttctggcgt
   108901 gacctgcgtg tagaccgcgt ttccggagga tctgacgaaa tgcagatcct gacgctgggt
   108961 cgtgcggtgc tgaagcaata ccgctaagtc cgtgcgctgc ctgatgcgac gctgacgcgt
   109021 cttatcaggc ctacgggagc acgaatgtag gccggataag gcgtttacgc cgcatccggc
   109081 agtcatgcgc tacataaatt tttcaggagc taataatgga tcatctaccc atgccgaaat
   109141 tcggaccgct ggccggattg cgcgttgtct tctccggtat cgaaatcgcc ggaccgtttg
   109201 ccggacaaat gttcgcagaa tggggcgcgg aagttatttg gatcgagaac gtcgcctagg
   109261 ccgacaccat tcgcgttcaa ccgaactacc cgcagctctc ccgccgcaat ttgcacgcgc
   109321 tgtcgttaaa tattttcaaa gatgaaggcc gcgaagcgtt tctgaaatta atggaaacca
   109381 ccgatatctt catcgaagcc agtaaaggtc cggcctttgc ccgtcgtagc attaccgatg
   109441 aagtactgtg gcagcacaac ccgaaactgg ttatcgctca cctgtccggt tttggtcagt
   109501 acggcaccga ggagtacacc aatcttccgg cctataacac catcgcccag gccttcagtg
   109561 gttacctgat tcagaacggt gatgttgacc agccaatgcc tgccttcccg tataccgccg
   109621 attacttttc tggcctgacc gccaccacgg cggcgctggc agcactgcat aaagtgcgtg
   109681 aaaccggtaa aggcgaaagt atcgacatcg ccatgtatga agtgatgctg cgtatgggcc
   109741 agtacttcat gatggattac ttcaacggcg gcgaaatgtg cccgcgcatg agcaaaggta
   109801 aagatcccta ctacgccggt tgcggtctgt ataaatgtgc cgacggctac atcgtgatgg
   109861 aactggtggg cattacccaa attgaagagt gctttaaaga tattggcctc gcacatctac
   109921 tcggtacgcc ggaaatcccg gaaggcactc agcttatcca ccgtatcgaa tgcccttacg
   109981 gcccactggt agaagaaaaa ctcgatgcct ggctggcggc acataccatc gcagaagtta
   110041 aagaacgctt tgccgaactg aatatcgcct gtgccaaagt actgaccgta ccggaactgg
   110101 aaagcaatcc acagtatgtt gcccgtgaat caatcactca gtggcaaacg atggatggtc
   110161 gcacctgcaa agggccgaac atcatgccga aattcaaaaa taaccccgga caaatctggc
   110221 gcggaatgcc ctcacatggc atggacacgg ctgccatttt gaaaaatatc ggctacagcg
   110281 aaaacgacat tcaggagttg gtcagcaaag gtctggccaa agttgaggac taaacgcgtg
   110341 gcgtcagtcg gcttgcctga ctagcgcccg cccaataaca aacgaacaaa aatggataga
   110401 ggtgcaatgg atatcattgg cggacaacat ctacgtcaaa tgtgggacga tctggcggac
   110461 gtttacggtc ataaaacggc gctgatttgt gaatccagcg gcggagtcgt taaccggtat
   110521 agttatcttg agttaaatca ggagattaac cgcacggcaa acctgtttta tacgctgggg
   110581 attcgcaaag gcaacaaggt tgcactacat ctcgacaact gcccggaatt tatcttttgc
   110641 tggttcgggc tggcaaaaat tggcgcgatt atggtgccga ttaacgcccg cctgttacgc
   110701 gaggaaagcg agtggatctt gcaaaatagc caggcgtgcc tgctggtgac cagtgcgcaa
   110761 ttttatccta tgtatcaaca gattcagcag gaagatgcca ctcaattgcg gcacatttgc
   110821 ctgacagatg tggcacttcc cgctgatgat ggagtgagtt cgtttactca actgaaaaat
   110881 caacaacctg ccaccttgtg ctatgcaccg ccgctatcga ctgacgatac ggcggaaatt
   110941 ctcttcacct ccggcaccac ctcccgaccg aaaggtgtgg tgattaccca ttacaacctg
   111001 cgcttcgctg gatattactc cgcctggcag tgtgcactgc gtgacgatga cgtctacatg
   111061 acggtaatgc ctgcgtttca tatcgattgc cagtgtactg cggcgatggc ggcgttttct
   111121 gccggggcca cctttgtgct ggtcgagaaa tacagcgccc gcgccttctg gggacaggcg
   111181 cagaagtacc gcgccaccat taccgaatgt attccgatga tgatccgtac gttgatggtg
   111241 cagccgcctt cagcgaacga tcggcaacac cgcctgcggg aagtgatgtt ttatctcaac
   111301 ttgtcggagc aggaaaaaga cgcattttgt gaacgcttcg gcgttcgctt gctgacgtct
   111361 tatgggatga cggaaaccat tgtgggcatt atcggcgatc accctagcga taaacgacgc
   111421 tggccgtcga ttggtcgggc ggggttttgc tacgaagcgg agatccgcga cgatcacaat
   111481 cgcccgctcc cggcaggtga gatcggtgaa atctgtatta aaggcgtacc agggaaaacc
   111541 atcttcaaag agtattttct caacccgaaa gccactgcga aagtgctgga agccgacggc
   111601 tggctacata ccggcgatac cggataccgc gacgaagaag gcttttttta tttcgtcgat
   111661 cgccgctgca atatgatcaa acgcggtggc gagaatgtct cctgcgtgga gctggaaaat
   111721 atcatcgcca cccatccgaa aattcaggac atcgtggttg tgggtattaa agattcgatt
   111781 cgcgatgaag ccatcaaagc atttgtggtg ctgaatgaag gtgaaacatt gagcgaagag
   111841 gaattttttc gcttctgcga acaaaatatg gcgaaattta aagtgccctc ttatctggag
   111901 atcagaaaag atctgccacg taattgctcg gggaaaataa ttagaaagaa tctgaaataa
   111961 atgaaacggc agggaactac cctgccagct aataatcacg ccctaaaaca atatgcattt
   112021 tttgcaggga tgttgtcgtc cctaaaaaag caaaaatgga gaaaaggaat gagtgaatca
   112081 ttacatctga cccgcaatgg atcaattctg gaaattaccc ttgatcgccc aaaagcgaat
   112141 gctattgatg caaaaaccag ctttgaaatg ggcgaagtat ttctaaattt ccgtgacgat
   112201 ccgcaattac gtgtcgccat tattaccggt gccggagaga aattcttttc cgcgggctgg
   112261 gatttaaaag cggcagcaga aggcgaagca ccggatgctg actttggccc gggtggtttt
   112321 gcgggattaa ccgaaatttt caatctcgac aaaccagtta tcgcagctgt gaacggctac
   112381 gcctttggcg gcggtttcga actggcgctg gcggcagatt ttattgtttg tgccgataac
   112441 gccagcttcg ccctgccgga agccaaactg ggcatcgttc ctgacagcgg cggtgtgctg
   112501 cgtctgccga agatcctgcc gcctaccatc gtcaatgaaa tggtgatgac cggcagacga
   112561 atgggcgcag aagaggcgct gcgttggggg atagtcaacc gcgtggttag ccaggcggaa
   112621 ctgatggata acgcccgcga actggctcag cagctggtta acagcgcccc gctggcgatt
   112681 gcggcgctga aagagatcta ccgcaccacc agcgaaatgc cggtagaaga agcgtatcgc
   112741 tatattcgca gcggcgtgtt gaaacactat ccatcggttc tgcattcgga agatgccatt
   112801 gaagggccgc tggcgtttgc cgagaagcgc gatccggtgt ggaaaggacg ttaacaaccg
   112861 tgagctatta cgcctttgag gggttaattc cggtggttca cccgacggcg tttgtccatc
   112921 ccagtgccgt cttgattggc gatgtgattg tgggagccgg tgtctacatc ggcccactcg
   112981 cctcactgcg tggtgactac gggcggttga tcgtgcaagc gggagccaat attcaggatg
   113041 gctgcattat gcatggctac tgcgacactg acaccatcgt tggggaaaac ggccatatcg
   113101 ggcatggagc gatcctgcat ggttgtgtga ttggtcgcga tgcattggtc gggatgaaca
   113161 gcgtaattat ggatggcgcg gtcattggcg aagagagcat tgttgccgca atgagctttg
   113221 tcaaagcagg ctttagtggc gagaaacgcc agttgttgat gggtacgcca gcccgcgctg
   113281 tacgcagtgt tagtgacgac gagttacact ggaaacggtt gaataccaaa gagtatcagg
   113341 atcttgtcgg gcgctgtcat gcatcgttac atgaaacgca gccactgagg caaatggagg
   113401 aaaatcgccc ccgtttgcag gggacgacgg atgtgacgcc taaacggtga tcttgccgga
   113461 tgcgacctgg ttgctggata agatgctaca acatcgtatc tggcaagcag gacgcccggt
   113521 atcaacgaga taaaattaac gacgcatact ctttgacaac atcatctgcc acttctgctc
   113581 gcggttaagt tccgtgagcg gaacggcagg aatacgcggt gtctttctta cgccgactaa
   113641 tttttcccga ctgttattgc gcaggcgcgc gtaaatttgc tcatcgatat cgaccacttt
   113701 aaccagtcgc tgacactggc atccccgccc ttccagctta ttagggatca tcttaacttc
   113761 gcagcttatt tctgtgactt ccgacagaat ataagtcagg gtattaaccg ccttgctgtg
   113821 ttcaatatcg aaatgaatag agatctctct tgctatcacc caccgatttt cagccatcat
   113881 ccattcggcg attaacaagt agagtagttt ttcaacatat ccttcacaca tacgaactcc
   113941 agtttcacaa attaaaagtc tggctatgac tcgttttgaa gcaattaaac aaggccatat
   114001 taaaattgtg gatatatcga tagtatgcaa cttcaccgtc gataagtgtg agctaaatcc
   114061 tgcttatgtc atcaaaaata tcgattcccc aaaagattta cttaatgggc aaaaaaaaac
   114121 ggtcctcatc agagaaccgt attagttatt cgatcaaatt agcggatgaa aaatatccgc
   114181 cacgacacgc tattatttga tctgtgcgtg catttcctgc accgaaatta ctttttcagt
   114241 cgcatcggca ttcagcgcca tcgcggtagc gaaaccaccg ttcagggtgg tgtcataatg
   114301 cactttatat tgcagcgcac tgcgacggat cacgcgggag tcttcaatcg cacgacggcc
   114361 tgaggtggtg ttgatgatgt aggtatattc gccattcttg atacggtcct gaatgtgcgg
   114421 acggccttca tgcaccttgt ttaccagacg cggattgata cccgcttcgc ccagcacaat
   114481 cgccgtgccg tgggtcgcat ccagctcgaa gccctgtttc agcagttttg ccgccaggtc
   114541 caccacgcgt tctttatcgc cttcgcgcac ggaaagcagc gcacgaccgt gtttcttcat
   114601 ggtggagttg ctgcccagct gcgctttggc gaacgcttca gcgaaggtgc ggcccacgcc
   114661 catgacttcc ccggtagagc gcatttctgg ccctaacagc gggtcaacgc ccgggaattt
   114721 attgaacggc agcaccactt ctttcaccga gtagtacggc ggaataactt ctttggttac
   114781 gccctgctca gccagcgatt tgccagccat cacacgcgcc gccacttttg ccagcggtac
   114841 gccggtggct ttggagacga acggaacagt acgcgccgca cgcgggttta cttcaatcag
   114901 gtaaacttcg ttgtttttca ccgcaaactg cacgttcatc agaccgcgca cctgcaattc
   114961 gaaagccagt ttctgcacct gctggcgcat cacatcctga atttcctgac ttaaggtgta
   115021 cgctggcagc gaacatgcgg agtcaccgga gtgcacgccc gcctgctcga tgtgctccat
   115081 gatgccgcca atcagcacca tttcgccgtc gcagatggca tccacgtcaa cttctactgc
   115141 gtcatcaagg aaatgatcca gcaacactgg cgcatcgtta gacacgctaa ccgccgtctg
   115201 gaagtagcga cgcaggtcag cttcgtcata gacgatttcc atcgcccgac cgccgagaac
   115261 gtaagacgga cgcaccacca gcgggtagcc aatctctttt gccttctcaa ctgccatttc
   115321 aatagcagta acggtggcgt tcgccggttg tttcagtttc agacggtcaa ccgcatgctg
   115381 gaagcgttca cggtcttccg cacggtcgat agcatccggg ctggtgccga taaccggtac
   115441 gccagcggct tccagtgcac gcgccagttt cagcggggtc tgaccgccgt actgaacgat
   115501 aacgcctttc ggcttctcaa tacgcacgat ttccagcaca tcttccagag ttaccggctc
   115561 gaagtagagg cgatcggaag tgtcgtagtc ggtagagacg gtttccgggt tacagttaac
   115621 cataatggtt tcgtaaccgt cttcacgcag cgccagcgag gcgtgtacgc agcagtagtc
   115681 gaattcgata ccctgaccga tacggtttgg accgccgcca agcaccatga ttttttcacg
   115741 gtcggtagac ggattcgctt cgcactcttc ttcataagtg gagtacatgt aagcggtgtc
   115801 ggtggcaaac tctgccgcac aggtatccac gcgcttgtag accgggtgca ggtcatattg
   115861 gtcacgcagc ttacggattt ccgcttcgcg tacgcccgcc agttttgcca agcgcgcatc
   115921 ggcaaagcct ttgcgtttca gctggcgcag gaattcagcg ttcaggccag tgatgcccac
   115981 ttccgccact ttctcttcca gacgcaccag ttcttcaatc tgtaccagga accagcggtc
   116041 aatgttagtc aggttgaaga cgccgtccac agacaggccc gcgcggaaag catcggcgat
   116101 gtaccagata cgctctgcgc ctgcgtcttt cagttcgcga cggattttgg ttaacgcttc
   116161 cgggtcatct aggctcactt tcgggtcgaa gccagttgcg cccacttcca ggccgcgcag
   116221 cgctttttgc agggattcct gctgcgtgcg accaatcgcc atcacttcgc caaccgattt
   116281 catctgagtg gtcagacggt cgttagcacc ggcgaatttt tcgaagttga agcgaggaat
   116341 tttagtaacc acatagtcga tggacggctc gaaggaggcc ggagtacgtc cgccagtgat
   116401 gtcgttcatc agttcgtcga gggtgtaacc caccgccagt ttcgccgcca ctttagcaat
   116461 cgggaaaccg gtcgctttcg acgccagcgc cgaagaacgg gacacgcgtg ggttcatttc
   116521 gataacaatc aggcgaccgt ttttcgggtt caccgcaaac tggacattgg aaccaccggt
   116581 ttcaacgccg atttcacgca gcaccgccat cgaggcgtta cgcatgattt gatattcttt
   116641 gtcggtcagc gtttgggctg gcgcgacagt gatggagtca ccggtgtgga tgcccatcgc
   116701 atcaaagttt tcgatagagc agacgatgat gcagttgtcg tttttatcac gcaccacttc
   116761 catctcgtac tctttccagc cgatcagcga ctcatcaatc agcaactctt tggtcggaga
   116821 gagatccaga ccgcgggcgc aaatttcttc aaactcttca cggttataag cgataccgcc
   116881 gccgctaccg cccatggtaa aggatgggcg aataatgcac gggaagccca cgtcagcggc
   116941 aacagccagc gcttcttcca tcgtgtgtgc gataccggaa cgcgcggttt ccagaccaat
   117001 tttcttcatc gctacgtcga aacgacggcg gtcttctgct ttatcaatcg catcggcagt
   117061 ggcaccaatc atggtgacac cgaactcttc caacacgccc tgacgttcca gctccagcgc
   117121 gcagttcagc gccgtctgac cgcccatggt tggcagcacc gcgtccgggc gctctttttc
   117181 aataatcttg cgtaccactt cccagtgaat cggctcgatg taggtcgcat cggccatttc
   117241 cgggtcggtc atgatggtcg ccgggttgga gttcaccaga atgacgcggt aaccctcttc
   117301 gcgcagggct ttacatgctt gcgcgccaga gtagtcaaac tcacacgcct gaccgataac
   117361 aatcgggccc gcacccagaa tcaggatact ttttatatct gtacgttttg gcatggctct
   117421 tttactcctg attacttagc ggttttacgg tactgctcaa ttaacttgat aaagtggtcg
   117481 aacaacggcg cggcgtcgtg tggaccaggg ctggcttcag ggtgaccctg gaagctgaac
   117541 gccggtttat cgatgcgatg aatgccctgt aacgtaccgt cgaacaggga tttatgcgtg
   117601 acacgcaggt ttgcaggtaa tgttgcttca tccaccgcaa aaccgtggtt ctgggcggtg
   117661 atcatcacca cgtttttctc cacatcttta accggatggt tgccgccgtg gtgaccaaat
   117721 ttcattttga cagtcttcgc accgctcgcc agcgccagca gctgatgacc gagacagatg
   117781 ccgaataccg gaatatcggt ttcgaggaac ttctggatgg cggtaatggc gtaatcgcac
   117841 ggggccgggt cgccaggacc gttggagagg aagatgccgt ctggattcat tttcagcaca
   117901 tcttccgcag aagtttgcgc cggaacgatg gtcaggcgac agcctctatc caccagcatc
   117961 cgcaggatgt tgcgcttggc accaaaatca taagccacga cgtggaacgg cagctcgtct
   118021 tcttttttcg cttctggcag gccgccggtc aacgtccagc tcccttgtgt ccagctatag
   118081 gcttctgcgg tggtcacttc ttttgccaga tccatgccgt tcagacctag gaacgcgcgg
   118141 gctttttcta acgccagcgc cgcatccggg ttatcgcctg cgataatgca gccattctgt
   118201 gcgcctttct cgcgcagtaa acgcgtcagc ttacgggtat cgatatcggc aatcgccacg
   118261 atgttatggc gcttcaggta agaagagagg tcttcggtat tacggaagtt gctggcaatc
   118321 agcggcaggt cgcgaatcac cagaccttgt gcatgtacct gagaagattc ttcatcggcg
   118381 tcattagtgc cgacattgcc aatatgggga taagtaagag taacgatttg acgagaatag
   118441 gaaggatcag tgaggatttc ttgataaccg gtcattgaag tattgaaaac gacttcccca
   118501 accgccgaac ctgttgcccc tatggcccga ccgtgaaact gggttccgtc ttccagaacc
   118561 aatagcgctg acttaatcaa aacaccctcc agagaatatt cactcacttt atttgcatat
   118621 taattcacag tgatgacgta aatcaatgca aatctgctta ccgacgaatt tctggcaaac
   118681 ggcggcattc tggagatatg ggcgctaaaa gtcaacttaa tggcaggatt ttttatatta
   118741 ttttgtggct catgcatgtt ttacaggcat taagcgacaa aaacatcatc taacctgttc
   118801 tggaaaacgc ttgcgcagca agaagattgc atgaaataaa aacgagcagg aaaaagtgga
   118861 ccaaatggtc aaaatttaca ttaaaacaac aaaaatagaa gacgaaacat gtaatttttg
   118921 gcagttatag aaaatttaaa agcataacac caaaaataaa agggctaaaa attaaagccc
   118981 tttataatta ataaattaaa tgtgttactt ttgcatcata ataaacattt tgtggttaca
   119041 aattattgag atcaagcaca tctcgcatat caaaaagacc actttcctta ccactcaacc
   119101 acaaagccga tcttaccgca ccgttagcaa atgtcatgcg gctggacgcc ttatgggtga
   119161 tctccagacg ctcgccaata tcggcaaaca tcgcggtatg ttcgccaacg atgtcacctg
   119221 cacgcacggt ggcaaaacca atggtaccag gcacacgttc accggtatgg ccttcacgac
   119281 tgtagaccgc gcaatctttc agatctttat caagggcgtg ggcgatcgcc tctcccattg
   119341 ccagtgcggt gcctgacggc gcatcaactt tatgtctatg atgtgcttca ataatttcga
   119401 tatcggtgta gtcacccatc actttggctg ctttctccag cagcttaagc atgacgttaa
   119461 cgccaacgct aaaattggcg gcaaagacaa tcgcaatatc ggcggcggcg tcacgaattg
   119521 cctgtttacc ggcttcgtca aaccccgtag tgccgatcac catccctttg ccatgctgcc
   119581 gacaaaaagc gagatggttc agcgtacctt ccggacgggt aaaatcgata aacacatcaa
   119641 aatcatcttt tatcgcatca agactgcttt gcacggtaac gcctgttttc cctgcaccag
   119701 ccagctcacc ggcgtcgctg cccagtaaag aagatccttc gcgctccagc gccgcgccca
   119761 gctgcacgcc ttctaatgcc agtgccgcct gaatcaactg gcggcccata cgccccccgg
   119821 ctcccgcgat ggcaacgcgg atgtttgcat catgcatagc tattctcttt tgttaatttg
   119881 catagaccgt tttcagagta accagcgcat cgctacgccg ccagccaaaa acgctgataa
   119941 ttagaaatta atgataaaca gggagggata tcagtgaaag gcatgagtcg atgaatgact
   120001 gcatgccgat aacatgtgac aggttacggc accagagcca gcacctcagc cacccactgt
   120061 tggaagcctt ttacattcag atccaatgcc acctgtacat tggctggttt gcccaggcaa
   120121 ccgtcgatat caaccaccgt cgtgcctgag gtaaattcgc cttgagtttc taccgcgaca
   120181 aaacacggct tgagggtgaa taagtccggg cgcaccagcc aggcgatggc gcagagatcg
   120241 tgcattcgca gaccgctttg catactgccg ctacggtagt ggctaaacag ggcgtggagc
   120301 atttttccgg tgcgattcaa ctccggtaaa gtagcgagat agtcaggggt taatattgcc
   120361 tgattggtga catccagacc gcacatgaca atttcgatgc cgctacggaa gacacaggca
   120421 gccgcttctg gatcggcagc gatattaaac tcggcatttg gtgtacagtt gccgcgacca
   120481 gcagaaccgc ccatgatcac cagacgacga atatacggtt tacattccgg gcattgtaaa
   120541 agtaacagcg caatattggt taacgggccg atggccacca gggtaacggg ctctggtgca
   120601 cgcatcaggg catcccgaat cgccagaaac gccggtatcc cgagtggctt tcggttgtgc
   120661 tcaacaaagt cgtagccagc cattcccgat tcgccgtgca cagatgctgc atcacgcggt
   120721 gcgcgtacca gcggcacagc ggctccctgg gcgagcggaa tctccgcatt ccagaaatgc
   120781 agcagttgca gggcattgcg ggtagttttc tcaaccgaga cattacccgc gacggtggtc
   120841 atcagttgca ggtcgagttc gggggcaaaa atcgcggcgg caatggcgac ggcatcgtca
   120901 atgccggggt cagtatcgag gaagataggt aaacgcatgt tttcttcata aaaaatgccg
   120961 gtaacaagac cggcattttc gcataactta ggctgctaat gacttaatcg acttcacgaa
   121021 tatcgacacg cagctctttc ggcacttcga aaacaatatt ttcttcacgg ccttccagcg
   121081 gaatggcttc accaccaccc aactgctgca aacgtgccac cacattctgc accagaatat
   121141 ccggagccga tgcgcccgca gtcacgccga cgcatttaac ctctttcacc cactcttcct
   121201 ggatatcttt cgcatcgtca atcaaaaacg cgtgtttgcc catacgctgg gccagctccg
   121261 ccagacggtt ggagttggag gagtttttcg aaccgaccac caacacaact tccgcctgtt
   121321 ctgccagggc gcgtaccgct tcctgacggt tagtcgtggc gtagcagatg tcatctttgc
   121381 gcggaccgac aattttcggg aagcgtttac gcagcgcgtc gatcacatca gacgtgtcat
   121441 ccaccgacag cgtggtttgg gtcataaagg agagcttctc ttcgtttttg accgtcagtt
   121501 tccacacatc gtctggcgat tcgaccagat acattccccc ttcggggttg ctgtactgcc
   121561 ccatcgtccc ttccacttcc gggtgcccgg cgtggccgat gagaatagat tcttcgccac
   121621 ggcgactggc gcgggcaact tccatatgca ctttggtcac cagcggacag gtggcatcaa
   121681 acaccgtcaa atcgcggctt ttcgcttcgt tacgaaccgc ctgagaaaca ccgtgtgcgg
   121741 agaaaatcag gatcgcgccg tccggcactt cgctaatctg ctcaataaag atagccccac
   121801 gctcgcgcaa gctatcgacc acgtagcggt tatgcaccac ttcgtgacgg acatatatcg
   121861 gtgcgccgta aatggccagc gcgttttcaa caatgctgat agcgcggtct accccggcac
   121921 aaaaaccacg cgggttggcc aacaggatct gcatgttacg cctccagtgc cggatcgatt
   121981 tccagcactt caatatcaaa atgaacggtc tgcccggcca gcggatggtt gaaatcaacg
   122041 gtaatggagt cgccgttaat ttcgcggatc acaccaggca tctcactgcc atccattgcg
   122101 gtaaaaagca tgattgcgcc aatttctggc tcgcctgcat ccataaattc acggcgggag
   122161 aagtactgaa tcaggtccgg tgacggcacg ccaaacgccg catctggctc cagcgagaag
   122221 gtggttttgt cgcccacttt cagccccagc aggtgttgct ccagcccttc agaaagagaa
   122281 gcatcgccca ggcggaacag cgccggttta ccgttgttgc gagtagactc ggcggtggtg
   122341 ccatcgtcga gttttagcgt gaagtgcacc aggacggcgc tattgctctg tacagattca
   122401 gacatgcagg tttgctctta tttttgagga tttttgccgg atgcggccta cgatttcgta
   122461 gcaatctgta ggccgcatcc ggcagggttt attattgttt tttcgctttg gaaggtaaaa
   122521 acccttccag cacgatcagt gccgcaccga cacaaatggc agtatcggca aggttgaagg
   122581 tggcgaagtg ccagtcgccg acgtagaagt cgatcatatc gacaacgaag ccgtgccaca
   122641 ggcggtcgaa caggttgccc agcgcgccgc caataatcag cgcgtaagcg atattgttta
   122701 gcttctgcgt ggccttcgag cgatacatca tcactgccag gagcacgcta ataccaatcg
   122761 caataccggc aaagaaccaa cgctgccagc cgccgctatc ggcaaggaaa ctaaacgccg
   122821 cgccatagtt acgcgcataa tgcagattaa gcgacgggaa cagcgggacc gtatccccca
   122881 gagcaaagtt ctggaggatc aggtatttgc tgcccagatc gataatcagc acgactacca
   122941 ccagccacag ccagcgtagc cctgttgaac agatcgattg actcatcagg caaacttacg
   123001 tttttcaccg tcaccggcga cgttgctgac acagcggccg cagatttctg cgtgttccgc
   123061 caccttgccg acatcctggg tgtagtgcca gcagcgtggg cacttctcac cttcggcttt
   123121 actcaacgcg actttcagcc ctttgagtac ttcgctctgc tgagcatcag caggtgcgtc
   123181 gttatagtct gcaacggtag cgccggaggt caacaggaca aatcgtaatt catcgcccag
   123241 cgcggtcagt ttcgccgcca gttccggttc tacatacaag gttactgccg cttccagcga
   123301 gccacccact ttcttgtcgg cacgcgcttg ctcaatgact ttgttcactt cgccacgcac
   123361 tttcaacagc tcgtcccaga acgcatcgtt cattgcttca ctgtctgcca gaccaaacag
   123421 gccttcgtac cactcaccgg tgaagacgta tttttcacgt tcgcccggca gatagcccca
   123481 cacttcatca gcggtgaagg agaggattgg tgccatccag cgcaccagcg cttcagcgat
   123541 gtgatacagc gcagtctggc agctacgacg cgccacgcta tccgctttgg cggtgtactg
   123601 acggtctttg atgatgtcga ggtagaagga acccatctca acggagcaga agcgcatcag
   123661 acgctgtacc acttcgtgga aatcgtatgc ttcgtacgcc ttgaggatgt cttcctgtgc
   123721 cgctttcgca caacctacgg cccagcgatc cagtaccacc atctcttccg gtttcaccat
   123781 atcttttgct ggatcaaaac cattcaggtt tgccagcagg aagcgcgcgg tgttacggat
   123841 acgacgatag ctatcggcag cacgtttcag gatctcgtca gaaacggcca tttcaccggt
   123901 gtagtcggtt gatgccaccc acagacgcag aatatccgcg cccagtttgt tcatcacatc
   123961 ctgcggcgaa acggtgttgc cgatggattt agacatcttg cgaccctgac catccacggt
   124021 aaagccgtgg gtcagtacct gacgatacgg cgctttgcct ttcatcgcgg tggagatcat
   124081 tagggaagac atgaaccaac cacggtgctg gtcagaacct tccagataca tgtccgctgc
   124141 gtgaccggca aattccggac gcacgtcaac aacagaagag tgggtagatc cggagtcaaa
   124201 ccatacgtcg agagtatccg gcactttcac atactggtca gcttcgtcgc cgaggatctc
   124261 tttcgcatcg agatcccacc acgcctggat gccgtcaact tcaacgcgtt tagccacttc
   124321 ttccatcagt tcgagggtac gcggatgcag ctcttccgtg tctttgtgca cgaacagtga
   124381 catcggtacg ccccaggtgc gctgacggga gatacaccag tcaggacggt tagcaaccat
   124441 cgactcgata cgcgcctggc cccagtccgg gatccactgc acgcctttga tctctttcag
   124501 tgactgcgca cgcagacctt tctgatccat gctgacgaac cactgcggtg tcgcgcggaa
   124561 gatgatcggc gttttgtgac gccagcagca cggatagctg tgctgcattt tctcaacgtg
   124621 cagcagcgcg cctttttcct gcagcagcgc aacgacgatg tcgttcgctt tgaagacgtt
   124681 cacgccatcc agcgtcggat aagtgcccgg cagataagtg ccgtccgggc caaccgggtt
   124741 agcggtttcc aggccgtatt tctgaccgat cacatagtcg tccgggccgt ggccaggcgc
   124801 ggtgtgaacg gcaccggtac cggcatccag ggtaacgtga tcgccgagga ttgccggaac
   124861 gtcgaagccc ataaacggat gggtaaagcg cagcagctca agctctgcac cttttaccgt
   124921 gccgagaatg gtgtaatcgg tcacgccgat acgctgcatt acgctttcaa ccagatcttt
   124981 cgccagaatc acggcctgac cgtcgatctg caccagcgca tagtcgaaat caggtgcaat
   125041 agagattgcg cggttcgcag gcagagtcca cggcgtagtg gtccagatca ccagcgagat
   125101 tgggccgtta acgttgctta cgccaaattt cgctttcagc gcatcctgat cgaccgcctg
   125161 gaaagcgacg tcaatggacg gagaagtttt gtcgtaatac tcaacttccg cttctgccag
   125221 tgcagaacgg cagtctacgc accagtgcac cggcttcgcg cctttgtgca ggtgaccatt
   125281 gccgatgatt ttgcccagcg cgcggatgat gttggcttca gttttgaagt ccatagtcag
   125341 atacgggtgc gaccagtcgc ccagcacgcc cagacggata aagtctttgc gttgaccgtc
   125401 aacctgggtc gccgcgtatt cgcggcactt ggcgcggaac tcggcggcgg tgaatttctc
   125461 acccggctta ccgtattctt gctctacttt cagctcgatc ggcagaccgt ggcagtccca
   125521 gccaggcaca tacggcgagt cataaccgga aagccctttg gacttcacga taatgtcttt
   125581 cagaatcttg ttaaccgagt gaccaatatg aatgctgcca ttcgcataag gagggccatc
   125641 atgcagaatg aaggtttttt tgcctttttt agccgcacgg atgatgccgt acagatcatc
   125701 atcagtccaa cgcgccagca ttcccggttc gcgcttggcg agatcgccac gcatcgggaa
   125761 ccctgtttcc ggcaaattca gggttgattt atagtcactc atcagattct cggttccgtg
   125821 tttcggtttg attacataac aggcttaagc cggttttgtt agcccaaaaa attcgcgggc
   125881 ggttaattca tcacgcgcaa tctgcgcttt cagttcgtcc agcgacgcaa atcgctgctc
   125941 attgcgtatt tttttacgca gcactacttg tatatggcga ccgtaaaggt ccattgcaac
   126001 atctaacaaa tgcacttcca gctgctggcg aataccggca accgttgggc gtgttccgat
   126061 gtttgccacg ccgggtaacg gcttttcacc gaggcccagc acttctaccg cataaacccc
   126121 tttcaccggg gaaacctgac ggcgtagcgg tacattcgcc gtcgggaaac ctatagtgcg
   126181 ccctaattca tcaccgtgga ctacacgccc ggagatggca aacgggtgcc ccagtaaact
   126241 ttctgccaga gccagattgt catcagccag cgcctgacgc acggcggtgc tgctgatacg
   126301 cacaccacct tcgcaaaaag tttgcgtact ggtgatatcg aagccgtatt ccatgccagc
   126361 tttctgtaat aacaagaaat cgccttcacg accagcgcca aagcggaaat catcacctac
   126421 ggcaagaaat tttacgcgca aatgcttcac caaaagatcg ctgatgaaat tttgcgcggt
   126481 taacgccgca aaacgcctgt cgaaacgcac gcacagcacg taatcaacgc cacactctgc
   126541 aaggtaacgc agtttttccc gcagccgggt cagtcttgcc ggggctttat cggtagcaaa
   126601 cagttccagt ggttgaggtt caaaaagcat caccatcacc ggtaagttgc gcttgcgccc
   126661 ttcttcctgc aagccctgta acagcgcgcg atgaccgcga tgcacgccgt cgaaattacc
   126721 aatagtcagc acacaccctt cttgcggggc ctggctgaga ttatgtatgc cgcgtatcag
   126781 cttcatgtct ggctcaaaac agtgaaaatc gtccgagtat accttgtaca gcggtcaagg
   126841 ttaaccggcg attgagtacc gaaatcagaa agaagtggct tttcattgcc atggcgcaaa
   126901 tcacgggaag aaactgaccg cctgctgcaa tttttatcgc ggaaaagctg tattcacacc
   126961 ccgcaagctg gtagaatcct gcgccatcac tacgtaacga gtgccggcac attaacggcg
   127021 cttatttgca caaatccatt gacaaaagaa ggctaaaagg gcatattcct cggcctttga
   127081 attgtccata tagaacacat ttgggagttg gaccttggct aatatcaaat cagctaagaa
   127141 gcgcgccatt cagtctgaaa aggctcgtaa gcacaacgca agccgtcgct ctatgatgcg
   127201 tactttcatc aagaaagtat acgcagctat cgaagctggc gacaaagctg ctgcacagaa
   127261 agcatttaac gaaatgcaac cgatcgtgga ccgtcaggct gctaaaggtc tgatccacaa
   127321 aaacaaagct gcacgtcata aggctaacct gactgcacag atcaacaaac tggcttaatc
   127381 gccaatttgc tgaagctttg tgaaaaagcc cgcgcaagcg ggttttttta tgcctgctgc
   127441 ttttgtgatg cgtgagagaa cacactgccg gttgccagat agtcaaaaga gacatgccgt
   127501 tgtactatct gaactcttcg tggcttaaga atcgtagaac catcggctga tacattctga
   127561 acaagaatat ctactcaatc tccagactcc cccacaaaga atatggatat tgtgatacac
   127621 attgaggtat taaacttaag accactctat ttttgaatta aattcctata tactttaacc
   127681 attcataaat atctcctcat tagacc
//
LOCUS       DADLSA010000002        84680 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.002, whole genome shotgun sequence.
ACCESSION   DADLSA010000002 DADLSA010000000
VERSION     DADLSA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 84680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.002"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..869)
                     /gene="glxR"
                     /locus_tag="JN691_000111"
     CDS             complement(<1..869)
                     /gene="glxR"
                     /locus_tag="JN691_000111"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY7241655.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANH"
     gene            complement(969..1745)
                     /gene="hyi"
                     /locus_tag="JN691_000112"
     CDS             complement(969..1745)
                     /gene="hyi"
                     /locus_tag="JN691_000112"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAY7241656.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWIGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(1758..3539)
                     /gene="gcl"
                     /locus_tag="JN691_000113"
     CDS             complement(1758..3539)
                     /gene="gcl"
                     /locus_tag="JN691_000113"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAY7241657.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGDIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(3629..4444)
                     /gene="allR"
                     /locus_tag="JN691_000114"
     CDS             complement(3629..4444)
                     /gene="allR"
                     /locus_tag="JN691_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAY7241658.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(4522..5004)
                     /gene="allA"
                     /locus_tag="JN691_000115"
     CDS             complement(4522..5004)
                     /gene="allA"
                     /locus_tag="JN691_000115"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HAY7241659.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELSFA
                     "
     gene            5234..6160
                     /gene="allS"
                     /locus_tag="JN691_000116"
     CDS             5234..6160
                     /gene="allS"
                     /locus_tag="JN691_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAY7241660.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNSQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            6229..7323
                     /gene="mnmH"
                     /locus_tag="JN691_000117"
     CDS             6229..7323
                     /gene="mnmH"
                     /locus_tag="JN691_000117"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAY7241661.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(8034..10448)
                     /locus_tag="JN691_000118"
     CDS             complement(8034..10448)
                     /locus_tag="JN691_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7241662.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(10445..11131)
                     /locus_tag="JN691_000119"
     CDS             complement(10445..11131)
                     /locus_tag="JN691_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7241663.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            11102..11725
                     /gene="tesA"
                     /locus_tag="JN691_000120"
     CDS             11102..11725
                     /gene="tesA"
                     /locus_tag="JN691_000120"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HAY7241664.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            11715..12524
                     /gene="ybbO"
                     /locus_tag="JN691_000121"
     CDS             11715..12524
                     /gene="ybbO"
                     /locus_tag="JN691_000121"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY7241665.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            12585..13439
                     /gene="cnoX"
                     /locus_tag="JN691_000122"
     CDS             12585..13439
                     /gene="cnoX"
                     /locus_tag="JN691_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAY7241666.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPKEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(13502..14281)
                     /gene="fetB"
                     /locus_tag="JN691_000123"
     CDS             complement(13502..14281)
                     /gene="fetB"
                     /locus_tag="JN691_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAY7241667.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(14268..14945)
                     /gene="fetA"
                     /locus_tag="JN691_000124"
     CDS             complement(14268..14945)
                     /gene="fetA"
                     /locus_tag="JN691_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAY7241668.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            15091..16008
                     /locus_tag="JN691_000125"
     CDS             15091..16008
                     /locus_tag="JN691_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAY7241669.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            16005..16463
                     /locus_tag="JN691_000126"
     CDS             16005..16463
                     /locus_tag="JN691_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAY7241670.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(16464..16871)
                     /locus_tag="JN691_000127"
     CDS             complement(16464..16871)
                     /locus_tag="JN691_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HAY7241671.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(16996..18288)
                     /locus_tag="JN691_000128"
     CDS             complement(16996..18288)
                     /locus_tag="JN691_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7241672.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPCNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            complement(18291..19223)
                     /gene="glsA"
                     /locus_tag="JN691_000129"
     CDS             complement(18291..19223)
                     /gene="glsA"
                     /locus_tag="JN691_000129"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAY7241673.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            19485..21989
                     /gene="copA"
                     /locus_tag="JN691_000130"
     CDS             19485..21989
                     /gene="copA"
                     /locus_tag="JN691_000130"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAY7241674.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITRGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(22204..22545)
                     /locus_tag="JN691_000131"
     CDS             complement(22204..22545)
                     /locus_tag="JN691_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY7241675.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            22683..23477
                     /locus_tag="JN691_000132"
     CDS             22683..23477
                     /locus_tag="JN691_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7241676.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            23681..24160
                     /gene="ybaK"
                     /locus_tag="JN691_000133"
     CDS             23681..24160
                     /gene="ybaK"
                     /locus_tag="JN691_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAY7241677.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(24197..25849)
                     /locus_tag="JN691_000134"
     CDS             complement(24197..25849)
                     /locus_tag="JN691_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY7241678.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEYGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNNPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            26067..27287
                     /locus_tag="JN691_000135"
     CDS             26067..27287
                     /locus_tag="JN691_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7241679.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            27525..29201
                     /locus_tag="JN691_000136"
     CDS             27525..29201
                     /locus_tag="JN691_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAY7241680.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(29334..30638)
                     /locus_tag="JN691_000137"
     CDS             complement(29334..30638)
                     /locus_tag="JN691_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAY7241681.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPSHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNIPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            30790..31749
                     /gene="aes"
                     /locus_tag="JN691_000138"
     CDS             30790..31749
                     /gene="aes"
                     /locus_tag="JN691_000138"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HAY7241682.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            complement(31746..32708)
                     /gene="hemH"
                     /locus_tag="JN691_000139"
     CDS             complement(31746..32708)
                     /gene="hemH"
                     /locus_tag="JN691_000139"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAY7241683.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDEALKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(32840..33484)
                     /gene="adk"
                     /locus_tag="JN691_000140"
     CDS             complement(32840..33484)
                     /gene="adk"
                     /locus_tag="JN691_000140"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAY7241684.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            complement(33665..35539)
                     /gene="htpG"
                     /locus_tag="JN691_000141"
     CDS             complement(33665..35539)
                     /gene="htpG"
                     /locus_tag="JN691_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAY7241685.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(35649..36254)
                     /gene="recR"
                     /locus_tag="JN691_000142"
     CDS             complement(35649..36254)
                     /gene="recR"
                     /locus_tag="JN691_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAY7241686.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(36254..36583)
                     /locus_tag="JN691_000143"
     CDS             complement(36254..36583)
                     /locus_tag="JN691_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAY7241687.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     NINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(36636..38561)
                     /gene="dnaX"
                     /locus_tag="JN691_000144"
     CDS             complement(36636..38561)
                     /gene="dnaX"
                     /locus_tag="JN691_000144"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAY7241688.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     gene            complement(38690..39241)
                     /gene="apt"
                     /locus_tag="JN691_000145"
     CDS             complement(38690..39241)
                     /gene="apt"
                     /locus_tag="JN691_000145"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAY7241689.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(39394..39771)
                     /locus_tag="JN691_000146"
     CDS             complement(39394..39771)
                     /locus_tag="JN691_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAY7241690.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            39841..40368
                     /gene="priC"
                     /locus_tag="JN691_000147"
     CDS             39841..40368
                     /gene="priC"
                     /locus_tag="JN691_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAY7241691.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            40382..40543
                     /gene="rsmS"
                     /locus_tag="JN691_000148"
     CDS             40382..40543
                     /gene="rsmS"
                     /locus_tag="JN691_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAY7241692.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(40755..44117)
                     /gene="mscK"
                     /locus_tag="JN691_000149"
     CDS             complement(40755..44117)
                     /gene="mscK"
                     /locus_tag="JN691_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAY7241693.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(44245..44892)
                     /gene="acrR"
                     /locus_tag="JN691_000150"
     CDS             complement(44245..44892)
                     /gene="acrR"
                     /locus_tag="JN691_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HAY7241694.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            45034..46227
                     /gene="acrA"
                     /locus_tag="JN691_000151"
     CDS             45034..46227
                     /gene="acrA"
                     /locus_tag="JN691_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAY7241695.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            46250..49399
                     /locus_tag="JN691_000152"
     CDS             46250..49399
                     /locus_tag="JN691_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAY7241696.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFANDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            49945..50319
                     /gene="tomB"
                     /locus_tag="JN691_000153"
     CDS             49945..50319
                     /gene="tomB"
                     /locus_tag="JN691_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAY7241697.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     LGNRLFRCFVNATKENPASLSC"
     gene            50345..50563
                     /gene="hha"
                     /locus_tag="JN691_000154"
     CDS             50345..50563
                     /gene="hha"
                     /locus_tag="JN691_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY7241698.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            50735..51286
                     /gene="maa"
                     /locus_tag="JN691_000155"
     CDS             50735..51286
                     /gene="maa"
                     /locus_tag="JN691_000155"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAY7241699.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     ALGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            51402..51872
                     /locus_tag="JN691_000156"
     CDS             51402..51872
                     /locus_tag="JN691_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7241700.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            52036..53586
                     /gene="pdeB"
                     /locus_tag="JN691_000157"
                     /pseudo
     CDS             52036..53586
                     /gene="pdeB"
                     /locus_tag="JN691_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(53628..53981)
                     /locus_tag="JN691_000158"
     CDS             complement(53628..53981)
                     /locus_tag="JN691_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HAY7241701.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(54101..54197)
                     /gene="ffs"
                     /locus_tag="JN691_000159"
     ncRNA           complement(54101..54197)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JN691_000159"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            54360..54671
                     /locus_tag="JN691_000160"
     CDS             54360..54671
                     /locus_tag="JN691_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAY7241702.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(54702..55274)
                     /locus_tag="JN691_000161"
     CDS             complement(54702..55274)
                     /locus_tag="JN691_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7241703.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAINVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            55492..56352
                     /gene="tesB"
                     /locus_tag="JN691_000162"
     CDS             55492..56352
                     /gene="tesB"
                     /locus_tag="JN691_000162"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HAY7241704.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(56401..57687)
                     /gene="amtB"
                     /locus_tag="JN691_000163"
     CDS             complement(56401..57687)
                     /gene="amtB"
                     /locus_tag="JN691_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAY7241705.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATEAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(57717..58055)
                     /gene="glnK"
                     /locus_tag="JN691_000164"
     CDS             complement(57717..58055)
                     /gene="glnK"
                     /locus_tag="JN691_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAY7241706.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(58236..60017)
                     /locus_tag="JN691_000165"
     CDS             complement(58236..60017)
                     /locus_tag="JN691_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY7241707.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(60010..61782)
                     /locus_tag="JN691_000166"
     CDS             complement(60010..61782)
                     /locus_tag="JN691_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY7241708.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAVDAKDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFVLKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(61812..62357)
                     /locus_tag="JN691_000167"
     CDS             complement(61812..62357)
                     /locus_tag="JN691_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAY7241709.1"
                     /translation="MVFKPHRINCRDFTFKIELKEKKILRGRAMLDKIDRKLLALLQQ
                     DCTLSLQALAEAVNLTTTPCWKRLKRLEDDGILIGKVALLDPEKIGLGLTAFVLIKTQ
                     HHSSEWYCRFVTVVTEMPEVLGFWRMAGEYDYLMRVQVADMKRYDEFYKRLVNSVPGL
                     SDVTSSFAMEQIKYTTSLPIE"
     gene            complement(62424..63242)
                     /gene="cof"
                     /locus_tag="JN691_000168"
     CDS             complement(62424..63242)
                     /gene="cof"
                     /locus_tag="JN691_000168"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAY7241710.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATYCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            63342..65042
                     /locus_tag="JN691_000169"
     CDS             63342..65042
                     /locus_tag="JN691_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HAY7241711.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPS
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            65107..65802
                     /gene="queC"
                     /locus_tag="JN691_000170"
     CDS             65107..65802
                     /gene="queC"
                     /locus_tag="JN691_000170"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAY7241712.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(65854..66252)
                     /locus_tag="JN691_000171"
     CDS             complement(65854..66252)
                     /locus_tag="JN691_000171"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAY7241713.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(66346..66717)
                     /locus_tag="JN691_000172"
     CDS             complement(66346..66717)
                     /locus_tag="JN691_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7241714.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(66868..68739)
                     /gene="ppiD"
                     /locus_tag="JN691_000173"
     CDS             complement(66868..68739)
                     /gene="ppiD"
                     /locus_tag="JN691_000173"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY7241715.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(68931..69203)
                     /gene="hupB"
                     /locus_tag="JN691_000174"
     CDS             complement(68931..69203)
                     /gene="hupB"
                     /locus_tag="JN691_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAY7241716.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(69412..71766)
                     /gene="lon"
                     /locus_tag="JN691_000175"
     CDS             complement(69412..71766)
                     /gene="lon"
                     /locus_tag="JN691_000175"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAY7241717.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(71954..73228)
                     /gene="clpX"
                     /locus_tag="JN691_000176"
     CDS             complement(71954..73228)
                     /gene="clpX"
                     /locus_tag="JN691_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAY7241718.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(73354..73977)
                     /gene="clpP"
                     /locus_tag="JN691_000177"
     CDS             complement(73354..73977)
                     /gene="clpP"
                     /locus_tag="JN691_000177"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAY7241719.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(74224..75522)
                     /gene="tig"
                     /locus_tag="JN691_000178"
     CDS             complement(74224..75522)
                     /gene="tig"
                     /locus_tag="JN691_000178"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAY7241720.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(75866..76183)
                     /gene="bolA"
                     /locus_tag="JN691_000179"
     CDS             complement(75866..76183)
                     /gene="bolA"
                     /locus_tag="JN691_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAY7241721.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            76488..77066
                     /locus_tag="JN691_000180"
     CDS             76488..77066
                     /locus_tag="JN691_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7241722.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            77110..78585
                     /gene="ampG"
                     /locus_tag="JN691_000181"
     CDS             77110..78585
                     /gene="ampG"
                     /locus_tag="JN691_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAY7241723.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            79045..79992
                     /gene="cyoA"
                     /locus_tag="JN691_000182"
                     /pseudo
     CDS             79045..79992
                     /gene="cyoA"
                     /locus_tag="JN691_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
     gene            80014..82005
                     /gene="cyoB"
                     /locus_tag="JN691_000183"
     CDS             80014..82005
                     /gene="cyoB"
                     /locus_tag="JN691_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HAY7241724.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGASANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNCDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            81995..82609
                     /locus_tag="JN691_000184"
     CDS             81995..82609
                     /locus_tag="JN691_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HAY7241725.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMKIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            82609..82938
                     /locus_tag="JN691_000185"
     CDS             82609..82938
                     /locus_tag="JN691_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HAY7241726.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            82950..83840
                     /gene="cyoE"
                     /locus_tag="JN691_000186"
     CDS             82950..83840
                     /gene="cyoE"
                     /locus_tag="JN691_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HAY7241727.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            84025..84336
                     /locus_tag="JN691_000187"
     CDS             84025..84336
                     /locus_tag="JN691_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY7241728.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSVAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            84320..>84680
                     /locus_tag="JN691_000188"
     CDS             84320..>84680
                     /locus_tag="JN691_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HAY7241729.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVM
                     LGGARKYQKRGFGQDLLC"
BASE COUNT        19999 a        22507 c        21396 g        20778 t
ORIGIN      
        1 ttatggttag ccattaattc cagcgcctgc actaacgcag agtgatccaa ctggctgcca
       61 ccgtttgccg cacaggtatt aaataactcc tggcaggtcg cagtgtttgg caggttcagc
      121 gcaagtgctt tcgcactttg cagtgccagg ttgagatctt tctggtgcag agcgattttg
      181 aagcccggat taaaggtgcg tttaatcatg cgctcaccgt gaacttccag aatacgtgag
      241 gaagcaaagc cgcccatcag cgcctggcgc acacgtaccg ggtccgcacc ggcttttgaa
      301 gcaaatagca gggcttcaga aaccgcttca atattgagcg ccacaataat ctgatttgcc
      361 actttgcagg tttgaccatc gccgttaccg cccacgaggg tgatattttt accgagcagt
      421 tcaaacagcg gtttaacacg ttcaaatacc gcttcatcac cgccaaccat aatcgacaac
      481 gtcccttcac gcgcaccgat ttcaccgccg gagactggcg catcgagata atcgccgccc
      541 agttcattca cctgacgagc gaaacgctta gtttcaatcg gggaaatgga gctcatatca
      601 acaatggttt tgcccttcag cgaggctttg gtacaaccat tttcaccgaa cagaacttct
      661 tcaacctgag gtgtgtccgg caccataata aaaatgatgt ccgatgcttc cgttacctgg
      721 cgagcagttt caacactgac ggcacccagt gacagtaatt catcagcaac cggtccaatg
      781 gtcgtgacat gtaattgatg accggcacgc gccagattaa tggccatcgg tgtacccata
      841 atgcctaagc caataaatcc cagtttcata attaacctct tttaaatttc gcttttcctg
      901 aattcagaca acacgatccc ctgccaactt agcggcctaa aaaagcattg tctgaatagc
      961 gttacgtttt aacggtacgg atccatccag cgtaaaccag cttccgtggt ggtttggggt
     1021 ttatattcac acccaatcca accgttgtag tcagagtttt caattaccgt aaagagatat
     1081 tcgtaattaa tttctccggt tcccggttcg ccgcgatgcg gattatcagc aatttgcagg
     1141 tgaccaattt tatcggccca ctgagtcatg gtgttggtta attcaccttc catccgctgc
     1201 atgtgataaa tgtcatactg aattttcaaa ttgcagcaac caacatcatc aatcaatttc
     1261 agcgcctgcc gagttccggt gagatggaaa ccaggaatat caaaatggtt aataggttca
     1321 atcagtaata aaatatcttc tttcatcagc atattcgcgg cataacgcag gttttctaca
     1381 agcgttgcgt gaatctgttc actgctgaaa ccagccggcg ttttaccgac cagacagtta
     1441 atttttttat tacccagcgc acgggcataa cgaatcgctg ctgctacgcc atcccgaaac
     1501 tcttcttcac ggccaggaat acaggcaata ccgcgctcgc ccgccgccca gtcacctgcc
     1561 ggtaaattgt gcagcgtatg ttcgagttta ttactcgcca gtacctgttt taattcttca
     1621 atgtcgtagt cataaggaaa cataaattca acgccgcgaa aaccacactg cgcagctttc
     1681 tcaaaacggg cgagaaaatc atattcacca aataacatcg ataaattagc agagaaacgt
     1741 aacatcattt atctccctta ttcatagtgc atgaagcagg tttcagtcgg tgcatccgct
     1801 gcgttatcgg cgatatcttc aaattccatg acgttatcca gttcgctgcc catcgaaata
     1861 ttggtcacac gctcgagaat aacttccacg actaccggta cccgatattg cgccattaag
     1921 gctttcgcct gttcaaaggc tggcgcaata tcttccggtt tgaagacccg aatagcttta
     1981 caacctaaac cttccgctac ttttacgtgg tcaacaccgt agccattcac ttcactggag
     2041 ttgatattct cgaaagcgag ttgcacgcag tagtccatgt caaaagcgcg ttgtgactga
     2101 cgaatcaggc cgagataggc gttattcacc agcacatgga tgtacggaat gttgaactgc
     2161 gcgccaacgg ctaactcttc aatcaggaac tggaagtcaa agtcgccaga aatcgccacc
     2221 acattgcgtt tcggatcagc ggcacaaacc cccagcgccg ccggaatcgt ccagcctaac
     2281 ggaccagcct gaccacagtt gatccagtgg cggtctttaa agacatgcag catttgtgcc
     2341 gcagcgattt gtgacagacc aatagtggtg acataacaaa catcgcgacc aaaggctttg
     2401 ttcatctctt catacacgcg ctgcggtttc accggcacgt tgtcgaagtg ggttttgcgc
     2461 agcaaagtgc gtttacgctg ctggcagtcg gcgacccatt ctttacgaca cggcagacga
     2521 cccgcttttt gcatctcctg cgccacttca accagcagtg tcagcgccgc tttagcatca
     2581 gagacaatgc cgagatccgg acacagcacg cgaccaattt gcgtcggctc aatatcaata
     2641 tgaacgattt tgcgcccttc ggtgtatttc tctaccgaac cggtatgacg gttagcaaaa
     2701 cggttaccga taccaaacac catgtcggac gccagcaacg ttgcgttacc gtaacgatgc
     2761 gcggtttgca gaccgaccat cccggccatc agttcatgat cgtccgggat acagccccag
     2821 cccatcagcg ttgggatcac cggaacgctg gtcagttcag caaactgttg taacagtgca
     2881 gctgcgtcag cattgattac cccgcccccg gcaacaatca ccggacgttc ggcctggatt
     2941 aacatttcta cagctttttc gatctgcata cggctggcag caggtttgta gaccggcagc
     3001 ggttcgtaca tgtcaggatc aaactcgatt tccgcaacct gaacgtcgaa cggtaaatcc
     3061 accagtaccg gacccggacg accagaacgc atcaggtgaa atgcctgttg cagcacgcga
     3121 ggcaccagcg ccgcttcacg aactgtaacc gccattttgc tgaccggttt agcaattgct
     3181 tcaatatcta cggcctgaaa atcttcttta tgcagacggg cgcgcggtgc ctggccggta
     3241 atgcacagaa taggaatgga atcagcagaa gcggaataga gcgcggtgat catgtccgtg
     3301 cccgcaggac cggaagtccc cagacatacg ccgatatccc ctgccgttgc gcgggtataa
     3361 ccttccgcca tgtgcgaaac accttccaca tgacgcgcca gaatgtgacg aataccgccg
     3421 tgcttacgca tcgctgagta gaacggatta attgcagctc ccggaacgcc gaaggcggtg
     3481 gtgatacctt ctttctccag cacatacatt gccgcgtcaa cggctctcat ttttgccatt
     3541 ttattcctac ctctattaat tggaaaaatt ttccaacttt caaattttgt caaaaaaacc
     3601 cgctccgcag agcgggatgc gacagacact atggatgtgc tttcagtccc aacgccgtgc
     3661 tgatatcgcg ggcggtgtct ctgaccagct caccctgact gacaaaacga tcttctgtca
     3721 gtcttgatga aggcccggag atggagatag cggcaacaac gctaccgaca tcatcgtaaa
     3781 ttgctgaagc tatgcaattc agacctacaa catgctcttc tttatctacg gtatagccca
     3841 gttcacgcgc ttgttccagg tccttcagca aggtgggcat atccacaagc gtagttggcg
     3901 taaactgctg caaaccggtt tgcagaatga tgctcatcaa ctcctcttcc gccagcggat
     3961 aaagcagcgc tttgcccgca ccggaagcat gcagcggcag acgactgccc agtggcgcac
     4021 acatcctgac catcgattta cactctaact gaccaattaa taccgcttca ttgccgttac
     4081 ggatcgcgac attgaccgtt tcgccggaaa gtaacatcag gcggcgcata aacggcccgg
     4141 cgacggagag gacatcgcgg ttatggatgt acgccgcacc gacgttaaag acacctaatc
     4201 ctatatgcca ccagcctaat tgactgtcct gatagacaaa atccgctgct tgtaaaacct
     4261 tcagcaagcg aaaggtcgtg gagagcggca aatccagatt gagagaaata tcgctaaccg
     4321 acgaacttcc cccacttttt tccagatatt gcagaatcgc aattccccgc tctaacgcct
     4381 gtgcgccctt ctgtgccaca ggctccgcct gtcctggcct gccgcgccgt ctaacttccg
     4441 tcatacctac gttaactccg acctacaagt gagcataaac aatgaacaat tagtttatct
     4501 tatgcaagtc ggttgcaggc gtcacgcaaa actgagttcc tgttcaggaa tactttcaac
     4561 atcacagttg tcactgccgc cgcgatcgat ggtcagaaaa tcggtgacgc gctgccaggc
     4621 gaaaagtggg tgatgccaga cgttacgatg gtaattcacc ccctgttcac cgttggtgat
     4681 aaacgcccgc agcgttgaca ggtctggttt gtcgtcgcct aacgccacga ccaccacaaa
     4741 cacttcacct ttcattggga taaaggcctg agtgcccagc ggatgacgtt cgagttcgtg
     4801 aatggtcagc ggcagattcg ccggttgcgc gcggttaatg ctgataagcg tacggtcttg
     4861 ctcaagaatc tcaaccagcg ccaaatcgtg gtaacgctcc accaggccat tgttaatatg
     4921 gaaaaaatcc cgttgctgcg tttcgattac gtcgccataa gcactaaagg cttcctgact
     4981 taacggtaat acctgaagtt tcatcttttt gtccttgtaa cataattccg ttcaaccacc
     5041 gccgaccggt cagcttggtt tcaataacag gcaatctaaa accttaagag aacaacatca
     5101 atacaaaagt tggaatgttt ttccaaaact agatttagtc cacaaattga acagggctat
     5161 catgtaaaaa tactgataaa tcagaggata agaaaatcac gcaacttgcg gatttaaaag
     5221 gggatacctg aagatgttcg atccagaaac cttgcggact ttcattgcgg ttgctgaaac
     5281 aggaagtttt tcaaaagcgg cagaacgatt atgtaaaacc acggcgacga tcagttatcg
     5341 cattaaactt ctggaagaga ataccggagt agcgctgttt ttccgtacga ctcgtagcgt
     5401 aacgttgaca gcggctggcg agcatctact ttcccaggcc agagactggc tgagctggct
     5461 ggaaagtatg cccagcgagc tgcaacaggt gaatgatggc gtggaacgcc aggtgaatat
     5521 tgtcatcaac aacctgctct ataactccca ggccgtcgcc cagttgctgg cgtggctgaa
     5581 tgagcgttac ccctttaccc agtttcacat ctcccgacaa atctatatgg gcgtctggga
     5641 ctcgctattg tacgaaggtt tttcgctggc tatcggggtc acgggaactg aggcgctggc
     5701 aaataccttt agtcttgatc ccttaggatc ggtgcaatgg cgatttgtca tggcggcgga
     5761 tcatccgctg gcgaacgttg aagagccgct aacagaagcg cagttgcgtc gctttccggc
     5821 ggtcaatatt gaagacagcg cccgcacctt aaccaaacgc gtcgcctggc gattgccggg
     5881 gcaaaaagag attattgttc ccgatatgga aacgaaaatc gccgcccatc tggcgggtgt
     5941 tggcattggt tttttgccaa aatcactttg ccagtcaatg atcgataatc aacaactggt
     6001 cagccgggta atcccaacga tgcgccctcc ttcgccattg agtctggcat ggcgcaaatt
     6061 tggcagcggc aaagcggtag aagatattgt gaccttgttt acccaacgca ggccggaaat
     6121 cagcggattt ttagaaattt tcggcaaccc acgcagttaa aaatcaagtt tccttgcgcg
     6181 ttggcgcgta aaatagcgcg caagctgatt tgaggttcac ttttaactat gcaagagaga
     6241 cacacggaac aggactatcg tgccctgctg attgctgata cgcccattat tgatgttcgc
     6301 gcccctatcg agtttgagca aggcgcaatg cccgccgcta tcaatctgcc gttaatgaat
     6361 aacgatgaac gcgccgccgt tggcacctgc tataaacagc aaggctcaga cgcagcgctg
     6421 gcgctggggc ataaactggt ggcgggtgaa attcgtcagc agcgtatgta cgcctggcgg
     6481 gcagcgtgcc tgcaaaatcc gcaaggtatt ctctgctgcg cccgtggcgg tcaacgttca
     6541 catattgtgc aacgctggct gcatgaagcg gggattgatt atccgctggt ggaaggcggt
     6601 tataaggcac tgcgccagac cgcgattcag gcgaccattg aactggcaca aaaaccgata
     6661 gtgctgattg gcggttgtac cggcagcggt aaaacgctat tagtgcaaca acagccaaac
     6721 ggtgttgatc tggaagggct ggcgcgtcat cgcggttcgg cgtttggtcg cacgttacaa
     6781 ccacaactta gccaggcgag ttttgaaaac ctgctggctg ccgaaatgct aaaaaccgac
     6841 gcccgtcagg atttgcgcct gtgggtgctg gaagatgaaa gccggatgat cggttcgaat
     6901 cacctgccgg aatgtctgcg cgagcgaatg actcaggcgg cgattgcggt ggtagaagat
     6961 ccgtttgaga tccgtcttga acgcctgaat gaagagtatt tcttgcgtat gcatcatgat
     7021 tttacccacg cttatggcga cgaacagggc tggcaggaat attgcgaata cctgcatcac
     7081 ggactttcgg cgattaagcg tcggctgggg ttacagcgct ataacgaact cgctgcccag
     7141 ctggatacgg cgctgacaac gcaactcacc accggcagca ccgacggtca tctggcctgg
     7201 ctggtgccgt tacttaaaga atattacgac ccaatgtatc gctatcagct tgagaaaaaa
     7261 gcggaaaaag ttgtctttcg cggtgagtgg gcggaagtgg cggtatgggt taaggcgcag
     7321 tgatgggcgg tattctgaaa cggcgattgt cgtgagaaca aaatggttgc accagaatac
     7381 agatataaaa accgacatga cagtcggttt ttttattttt tccgcccaag acttggtggt
     7441 tccgggcaga aattaaacag tcctcatcta gcaagtgaat tataagtcag gatgcctaca
     7501 tattcaaaca gctatgttgg cgatacaatc ccgacgctag tagtgatact gcctcaaggg
     7561 attaccgcag tgcggaaaag tacttttaag tcctggtcag gatacaggtg gtgctgattc
     7621 tggtgctcta atttggagag aggcagtcct catctagcac atgaagaaaa tcatagttac
     7681 aggcaacgta agggtaagct gtgattgtct ttctccctta agggagcaac ggatgaaaac
     7741 ttttctcgat aaagttctca tcgttcttaa ggcgctaatc gcgctgctgg aactgatccg
     7801 tcagtttatt gaataactga ggaaacgtag ctaaggccga aggtttacct ccttcggcct
     7861 ttaaaaaaac cgacatgaca gttggtttca tatttattgc caaaatgcac actgttcatg
     7921 ccagatgcgg cgtaaacgcc ttatccggcc tacacaatct tgcaaaatca atatattgca
     7981 cgctcttcgt aggcctgata agcgtagcgc atcaggcaat ctaacacctg tcatcacccc
     8041 acaaactgcc tgaacagcgc cttaccctta accagtcgcg cacccagcca gccgccgaaa
     8101 agcgacagca gcagcgcacc gctgcacggc agaacaatcc acaatcgcca gtctggctcc
     8161 cacgggaaat caaacacttt cgcctgcaac actgccagtg ccgtttccgc accaattgcg
     8221 gccaccaggc cggaaacaaa cccaagcatg gcgaactcac accacaatgt cgtgcgcagc
     8281 agttttttcc ccgcaccgag tgtgcgccat accaccagct cctgatgacg ctggcgcatt
     8341 cccacctgca cctgtgccag caacagcaac ataccgcagg cggtgaccag caccaccatc
     8401 acctccagcg cccgacttac ctgctccagc acctgaccga cctgttttaa aatcgcgcca
     8461 atgtctaaca ggctaatggt cgggaactgg cggttgagtt gcgtcaacat gccgttgcca
     8521 ttctcccagc ggaaactggt aagccagctc tgcggttgcc cgtctaatgc cccttcaggg
     8581 aaaataaaat agaaattagg ccgcagactt tcccagtcca ctttgcgcag gctggtcact
     8641 ttagcgcgga actcctgggt atcgcccata aaagtcacgg tatcaccgag ggcaacgttt
     8701 aagcgtttcg ccagcccctc ttccatcgac acttcatcgg cttttggcgg ccagttaccg
     8761 gcgacaatcg gattatgatc gggccgcgta ttttgccagg taagattgag ttcgcgatta
     8821 agcgcctcat cttcattacc ttctgtcggc ttatcgttaa tcgccgtcag ccgcgcccgc
     8881 actaccggat aaaatgattc cgggactata tgatgttccg cgaggaacgc ttttagcggt
     8941 gctacctgtt ctgtggcgat gttaattaaa aagtagttcg ggctttctgg cggtagctgc
     9001 tgctgccagc ggtcgagcag atcgccacgc agcaccagca gcagtgccag cagcataaag
     9061 gagagcgaaa atgccgaaag ctggcttaac gttgaccacg gctgacgtaa caggcggcta
     9121 accgccaggc gcagaggcag cgatttcagc gtcatgcggc gaagtacatt cagcagcatc
     9181 cagcccagca caccgcacag caaagccagt actaccgcgc ccgccagcac cgcccaaagc
     9241 agcatgctgc cacccattaa tccggcgagc agcagcacaa ccaccacact gacaatcggc
     9301 agataaaact tcagcggcca gacgttcgcg accacatcat tacgtaatac gcgtaaaggc
     9361 tgcgttgcca gcaacaagcg gtatggtcgt agccccacca gcagcgagat gaccgtcatg
     9421 gtgccaagcg cccacagcca cggccagagg ctggctggcg gtagtgcagc aggcagaacg
     9481 ggcttgagca gcaccatcaa cacgttttcg aacaacaggc ctatggcccc accggtaacg
     9541 gctgaaagcg tcagcaccat caaccactga ccgacgatta gcttacgcag ttgcgctcgc
     9601 cctgccccca gcgttttgag gatcgccacc agatcgtagc gactgcgaca gtaatgattc
     9661 atcgctaccg ccaccgctgc cactgccagc agcaaggtca gaagcgccga aagcagcagg
     9721 aactgttgcg agcgttccat cgatcgcccc agcgcgcctt cgtcctgttc cagaccgtac
     9781 cagcgttgtt cgggtttaag ctgaggtaac aaccatttct catagccgtc gagctggttc
     9841 tcgttgccgc cgaatttata acgccaggtg acccgactcc ccggctgcac ggctccggtt
     9901 ttatcgacat ccgccagatt catcatcaga cgcggagcca tctggaaggg gttaaaaccg
     9961 gaatccggtt cctgaatcac ttctccggca atccgcaagg tggcatcgcc cacgtcaatg
    10021 gtgtcgcccg ttttcaggtt aagcagtgcc atcaggcgtg gggccagcaa tacgctgccc
    10081 gcctgcggtt tcaggccagg gggattagtt tgcagatcgc catacatcgg gtagatatca
    10141 tccaccgctt tgacgttcgc cagctgcggt gtgtcgcctg caaaggtcat tgtggcgaaa
    10201 gtcagctgct tgccgacttt caggccgcgc ttttgcgctt cctccagcca cgcttgcggc
    10261 acttcgcgtg aactgcgcaa cgcccgatcg cccgccataa actcacggct ttgctggctt
    10321 aagcccttct ccatgcgatc gctgatattg cccagcgcca gcacgcaggc caccgccagg
    10381 cttagcgcca gccagacaat taatagcgac ggcgaacgcc attcgcgcca gaaccaacgt
    10441 gcaatcatgc ttcctcctgc aactgcccgt tcaccagccg taagcagcgg tcgcagcgtg
    10501 ccgccagttg caggtcgtgg gtcaccataa tcaacgtggt gccatgttca cggttgaggg
    10561 aaaacagcag gtcggcaatt ttatcgcccg tctggcggtc aaggttgccg gtgggttcgt
    10621 cggcaaacag cacatcaggt cgaccattaa aggctcgtgc cagcgccact cgttgctgtt
    10681 caccgccgga aagctgcgcc ggaagatgat ccagacgttt acccagccct aactgttcga
    10741 gcaacgcttt cgccccgtta cgactttccg cgctactctc accgcgcagc agagccggaa
    10801 gctcgacgtt ttccagcgcg ttaagggtag gaattaacat aaatgactga aaaacaaagc
    10861 cgacgtgctt cgcgcgcaac tttgcccgcg cttcttcgtc catattatgt agcggttgtc
    10921 ccaccagact cacttcgcca ctgctgccgt catcaagccc ggcgaggatc gccagcaagg
    10981 ttgacttacc cgatcccgac tcgcccacca gtgcgatggt ctcgccacgt ttgacaacca
    11041 gctcaactcc ggtgaggatg gagagttcat gctccccctg accgacggac ttcttaagat
    11101 gatgaacttc aacaatgttt tccgctggca tttgcccttc ctgttcctgg tcctgttaac
    11161 cttccgtgcc gccgcagcgg acacgttatt gattctgggt gatagcctga gcgccgggta
    11221 tcgaatgtct gccagcgcgg cctggcctgc cttgttgaat gataagtggc agagtaaaac
    11281 gtcggtagtt aatgccagca tcagcggcga cacctcgcaa caagggctgg cgcgccttcc
    11341 ggctctgctc aaacagcatc agccgcgttg ggtgctggtt gaactgggcg gcaatgatgg
    11401 tttgcgtggt tttcagccac agcaaaccga gcaaacgctg cgccagattt tgcaggatgt
    11461 caaagccgcc aacgctgaac cattgttaat gcaaatacgt ctgcctgcaa actatggtcg
    11521 ccgttataat gaagccttta gcgccattta ccccaaactc gccaaagagt ttgatgttcc
    11581 gctgctgccc ttttttatgg aagaggtcta cctcaagcca caatggatgc aggatgacgg
    11641 tattcatccc aaccgcgacg cccagccgtt tattgccgac tggatggcga agcagttgca
    11701 gcctttagta aatcatgact cataaagcaa cggagatcct gacaggtaaa gttatgcaaa
    11761 aatcggtctt aattaccgga tgttccagtg gaattggcct ggaaagcgcg ctcgaattaa
    11821 aacgccaggg ttttcatgtg ctggcaggtt gccgaaaacc gaatgatgtt gagcgtatga
    11881 acaacatggg atttaccggc gtgttgatcg atctggattc accagaaagt gttaatcgcg
    11941 cagcagacga ggtgatcgcc ctgaccgata attgtctgta tgggatcttt aacaatgccg
    12001 gattcggcat gtatggcccc ctttccacca tcagccgtgc gcagatggaa cagcagtttt
    12061 ccgccaactt tttcggcgca caccagctca ccatgcgcct gttacccgcg atgttaccgc
    12121 acggtgaagg gcgtattgtg atgacatcat cggtgatggg attaatctcc acgccgggtc
    12181 gtggcgctta cgcggccagt aaatatgcgc tggaggcgtg gtcagacgcg ctgcgcatgg
    12241 agctacgcca cagcggaatt aaagtcagcc tgatcgaacc cggtcccatt cgtactcgct
    12301 tcaccgacaa cgtcaaccag acgcaaagtg ataaaccagt cgaaaatccc ggcatcgccg
    12361 cccgctttac gttgggaccg gaagcggtgg tcgacaaagt gcgccatgct tttattagcg
    12421 agaagccgaa gatgcgctat ccggtaacgc tggtgacctg ggcagtaatg gtgctcgagc
    12481 gcctgctgcc tgggcgcgtg atggacaaaa tattgcaggg gtgagttgaa gcgcgcgctt
    12541 aagcccccat gtcaaagaaa atatcgacaa cagagagcga ctccatgtcc gtagaaaata
    12601 ttgtcaacat taacgaatct aacctgcaac aggttcttga acagtcgatg accactccgg
    12661 tgctgttcta tttttggtct gaacgtagcc agcactgttt gcagttaacc ccgatcctgg
    12721 aaagcctcgc ggcgcagtac aacgggcaat ttattctggc gaagctggac tgcgacgcgg
    12781 agcagatgat tgccgcccag tttggtctgc gcgcgattcc gaccgtgtat ctgttccaga
    12841 acgggcaacc ggtagatggc ttccaggggc cgcaaccgaa agaggcgatc cgcgccctgc
    12901 tggataaagt gctgccgcgc gaagaagagc tgaaagcgca gcaggcgatg caactgatgc
    12961 aggaaggcaa ttacaccgat gccctgccat tgctgaaaga cgcctggcag ttgtcgaatc
    13021 agaacgggga gatcggcctg ctgctggcag aaacgctgat tgcgctgaac cgttctgaag
    13081 atgcggaagc ggtgctgaaa accattccgt tgcaggatca ggacacccgc taccaggggc
    13141 tggtagcgca aatcgaactg ctgaagcagg cggctgataa gccggaaatt caacagttgc
    13201 aacagcaggt ggcggagaat ccagaagatg ccgcactggc gacgcaactg gcgctgcaac
    13261 tgcatcaggt tgggcgcaat gaagaggcgc tggagttgct gttcgggcat ctgcgtaaag
    13321 atctcaccgc cgcagacggt cagacgcgta aaacgttcca ggagatcctc gctgcgctgg
    13381 gtacgggtga tgcactggcg tcgaagtatc gccgccagct gtatgcattg ttgtattgat
    13441 attccgttgt tcggcacatg aaccacaacg ccggatgcga tgctgcgcat ccggcatcgc
    13501 atcatttctt cttcaattga gtcaccacca actggtggcg cgaattataa aacttacgat
    13561 aggttaaata gcaggcaata atggtcgaca agctggcggt tgagagcagc ataaaggtca
    13621 ccataatctg atatttaatc gccttcaccg gatcaatccc ggcaaatatc agcccggaca
    13681 tcattcctgg taaactcact aagccaaccg tttttgctga atcgaccgtc ggaattaaag
    13741 ccgcgcgaat actgtcgcga atcaatatcg ctgaagcctg cttaggcgtc gcgccaagac
    13801 tcagtttctc ctgaatctgt tgctgttcgc taatgacccg ttgccctaaa ttgttgtaac
    13861 acaaccccac cgctaccatg gcgttaccgg caatcatccc ggcgacaggg atcacctgca
    13921 tcgggataaa ttcaatcgac cctgagagaa tcagcaccgc cagggtaatt cccgccccga
    13981 ccgtaatggc aataaacgat gagataaaag ctttagcaat atatttactg cgtttttgcg
    14041 cgttccacgc cgcattaaag cagataaata acaccatcaa taatgtcagg ctggcatcat
    14101 ccacgctgaa aatatacttc agcacatagc cgacaataat cagctgaatt atcgctcgcc
    14161 cgacgctcca gagaatatct ttctccagcg ccagtttttc tttatggcta attaagattg
    14221 ccaccaccac cagcattaat gccagtgcta atgattcgtt agtaatatta tgcgagttca
    14281 tagcgtgctt cctgcatttc tccggcatgc ggttgcagtg taatcacttt atccgcatga
    14341 ttaatttcgt ctttatcgtg tgtcacccac agcacggcaa tattttgctc gcgcacataa
    14401 cgatggatca tctcattgac gttatgttta ttactttcat ccagcgcact ggttatttca
    14461 tccagcaata aaaccttcgg cataaattgc aggttacgaa tcaatgagat gcgttgtttt
    14521 tcaccaccag atagctcggc gatattcttc gtcaaaatgc tgtccggcaa agcgaagcgt
    14581 tcgagaaaat cgagaaaaat ggccgggtca ggctgctggt cacggatctg ccagggaaag
    14641 atcagattat cgtataccgt atcgccaaac agcgtcggcg tctgggcgca gtaagagact
    14701 tgctggcggt agatttctgg ttttagtgtg ctgacatcct caccttcaaa cagtaacgtt
    14761 ccgctggttg ggctgatcaa tgaagcaact atttttagca gcgtactttt gccacaacca
    14821 gaaggaccgg taattaactt aaattcgcca gcacgcagcg aaaaattgat gttattaaga
    14881 atcttcgtat cacccgccag atatcctacg ttttgtagct gaagcaaagg actgttttcc
    14941 tgcatcgctg ttcccttttt ctgattttta ctaaaaacag tttatccttc gcaggaataa
    15001 gggggaactc tctttcagta atcaggtata atttgtttaa tcaagcagca attttgctga
    15061 ttatggacgg tacaacagga ggtttttccg atgcttatct ttatcccgat tctcattttt
    15121 gtcgcgctgg tcattgtcgg cgcgggcgtc aaaatcgtgc cgcagggcta tcaatggacg
    15181 gtagaacgtt ttggtcgcta taccaaaacg ttacagccgg ggctcagtct ggtggtgccg
    15241 tttatggatc gcattggtcg caagatcaat atgatggagc aagtgctcga tatcccttcc
    15301 caggaagtta tctcgaaaga taacgccaac gttaccatcg acgcagtctg ttttattcag
    15361 gtgattgacg cgccacgcgc tgcctatgaa gtcagcaatc tggagctggc gatcatcaac
    15421 ctgaccatga ctaacatccg taccgtgttg ggttcaatgg aacttgacga aatgctctct
    15481 cagcgcgaca gcatcaactc acgcctgctg cgtattgtcg atgaagccac caacccgtgg
    15541 gggattaaag tcacccgtat tgaaattcgc gacgtgcgcc caccggcaga gcttatctct
    15601 tcaatgaacg cgcagatgaa agcggaacgt accaaacgcg cttacattct tgaagcggaa
    15661 gggatccgtc aggcggaaat cctcaaagcc gaaggtgaaa aacagtcgca aatcctgaaa
    15721 gcggaaggcg aacgtcagtc ggcgttttta caggctgaag cgcgtgaacg ttccgccgaa
    15781 gcagaagccc gcgccaccaa aatggtgtct gaggctatcg cctccggtga tattcaggcg
    15841 gtgaactact tcgtagcgca aaaatacacc gaagcgttac agcagatcgg ttcctccagt
    15901 aacagcaaag tagtgatgat gccattagag gccagcagcc tgatggggtc gattgccggg
    15961 attgccgagc tggtgaaaga cagcgccaac aaacggactc agccatgatg gagttaatgg
    16021 tcgttcatcc acatattttc tggctcagtc tcggcgggtt gctgctggca gccgagatgc
    16081 tgggcggaaa tggttatttg ttgtggagcg gcgtggcagc ggtgattacc ggcctggtgg
    16141 tctggctggt gccgctgggt tgggagtggc aaggggtaat gtttgccatc ctgacgctgc
    16201 tcgccgcctg gctgtggtgg aaatggttgt cgcggcgggt gcgcgaacaa aagcacagcg
    16261 atagtcattt aaaccagcgc gggcagcagc tgattggccg acgttttgtg ctggaatctc
    16321 cgctggtcaa cgggcgcggt catatgcgcg tcggtgacag ttcatggcct gtcagcgcca
    16381 gcgaggatct cggtgcaggt acgcatgttg aagtcattgc gatagaaggg ataaccctac
    16441 atatccgagc cgtctcgtct taatcaccct gcccgatgat gacagcagcc ggagagattt
    16501 tcgataatcg ggcagtcggc gctgtcatcg ccagggcagg cattcgccag tgccagcagc
    16561 tggtcgcgca tggattgcag ctcctcaatg tgtcgttcga tctccgccac cttctccagc
    16621 gtgcgccgtt tgacgtcggc gctgtgccgc tgcgggtcgt taaacagatt caccagctcg
    16681 ccgctctctt ccaggttaaa gcccacctgc cgtgcctggc gcagtaaggt cagttcgttg
    16741 agatgctgct gcgtgtaggt gcgataaccg ttttcgctgc gcatcggcgg cgtcaccagc
    16801 cccttctctt catagaagcg aatggctttg ctggtcaggc cggtaatttt tgctacatcg
    16861 ctgatgttca tcgttcgcgc aacgcctcct gtgctttgtt aaagggtttc aacagatatt
    16921 caagcacgtt tttttgccag tgcgaatatc gaccgccacc acaataatac aggaagtact
    16981 gcgcacagag aggtgttata cggttttatt acgcttcatg accattgcca caataaggct
    17041 gagtaagatt gcaccaataa tccatatcaa cgcacggctg ccctgggacc acaggctgta
    17101 gataaagcca acgatcacca acaacatcac caacgtacca acgataagaa tcggcaacga
    17161 ggcgtgaata tcatgacgca ggcggatcgc caccacaaac accgccagat agcagatcaa
    17221 aaaggtggcg ctggcaacgc tggcgagtga gtctaaattt agcgccgccg tcatcagcat
    17281 tatcaacacc acgacgatga tgttgcccca ggtactctgc cgccacaggg atttattcat
    17341 tagcttcggc agttcgcgtt cgctgcccat gttgtccatg atgttaaaca cggcgaacag
    17401 gttcgcgtta atgcctgaag ccgtcgccag taaagcgccg atgacgacga tcacataacc
    17461 cacatgcccg agcagcggag aagcagcctg cgctacggcg gtatcagcat atttttctaa
    17521 ctctaatgcc gatacatcgc taagcaaaac cagcgccagc gagatataaa gcaacgtggt
    17581 aacgccaatc gccaccagaa acgcccgtgg cataatgacc tgcggatctt tcactttatc
    17641 cgccgcgttc gccatcatgc caaagcccgc ataggcaagg aaggttatcc caatacagga
    17701 gaagaacgca ccggagctgg ggggcgcaga gacggaaata tgcgccggtt gcagcgacca
    17761 gacaccggca ataatcagca ataacaggat catcatttta atgccgacga ggatcacttc
    17821 cagccgccct accgcatggt tgcttaagga gttgaaaagc gtcatcaccg caatgatccc
    17881 caacgcgtag agcaaaataa ggtgctcctc ctggctgcct tcatgcaaaa actgcacggc
    17941 ataagcgcca aaagcacggg caaccatggc gatgctcacc gccagcgtca acaggtatag
    18001 caacgagagc gctagcgaaa agacgccgtt gcctaatccg cgacgaaaaa agtcgataat
    18061 accgccattg cagggatagc tcgcccccag acgcgcatag gcataaccgg aaaacatcgc
    18121 cacaataccg ccaaaagcaa aggcgcccca ggtcgaggct tccattagca acgcagcctg
    18181 ccccagcagc gcgaagatcc ccgcccccac cattgcccca ataccgatgg aaacgacgtt
    18241 ccatagaccg aggggtttgt taccgttatt accttccgtg ttcatcatga tcagccctta
    18301 aacacgttat agccgagttg cttagcgacc gatgccacca ttttttgacc gcgaacacta
    18361 ttgccttctt cgtccagcgg tggtgagaac gcggcaattc ccatcacgcc agggacgacc
    18421 gccagaatac cgccacctac accgcttttg cccggtaaac caacgcgata cgcccagtca
    18481 ccggagcgac catacagccc ttccatcatc atttcggcca gaatgtacgg cacgttgtcg
    18541 gcctgaagaa cgcgtttatg cgtcaacgga ttcacaccac ccgccgccag cgtcgcgcca
    18601 agcgttgcca gttcaacagt attgatgagc gtagagcact gacgggtgta cacgtcacag
    18661 gcttccattg catcacaata gagatatccg gcggagtaca gcagccaggc aatggcccgg
    18721 ttatggaagt tggttgtttg ttccgactgg ttgacttcgt cagagagcgc tacctgctca
    18781 ccagccagtt gctgttggat atgtaaaatt cgctgccagc gttgttcggc gttttcagcg
    18841 ttaatcaggc tggtggtggc aatagcgcca gcatttacca gtggcgaaag cggtttgccg
    18901 ccatgcaact ctaaggcgat aactgagtta aagggcaatc cggtcgggtc agcgccaatt
    18961 ttgtcctgta ccgcctgcgg gccgacatct tctaacgcaa gggctaacgt acagacttta
    19021 gagatggatt ccagtgcaaa gcggtaatca ctgtcacccg cactatagac gttgccatcg
    19081 caggtcacga tagccactgc cgccagttga cctggtacat tcgccagaaa gggaatgtaa
    19141 tcggcatttt gtccgccgtt aagtgagtga aattgggtgt aagcctgatc cactgcctgc
    19201 tgtaatttgt ttgcatctaa catcttttgt taactccttt ttatagatgc gggaggtaat
    19261 tcctcacccc ggtgccgatt ttcaggcatc ctgatttaac ttagcacccg caacttaact
    19321 acaggaaaac aaagaaataa atgtctaatc ctgatgcaaa tcgagccgat tttttaatct
    19381 ttacggactt ttacccgcct ggtttattaa tttcttgacc ttccccttgc tggaaggttt
    19441 aacctttatc acagccagtc aaaactgtct taaaggagtg ttttatgtca caaactatcg
    19501 acctgaacct ggacggcctg tcctgcggtc actgcgttaa acgcgtgaaa gaaagtcttg
    19561 aacagcgccc ggatgtggaa caagcggatg tgtctatcac tgaagcgcac gttaccggga
    19621 ctgccagtgc agaacagctg atcgaaacca tcaaacaagc gggttatgac gcatctgtaa
    19681 gccacccaaa ggctaaaccg ctggcggagt catcaatccc gtcggaagca ctgacagcgg
    19741 tttctgaggc gcttccggca gcgaccgccg atgacgatga cagccagcag ttgctgctga
    19801 gcggcatgag ctgcgccagc tgtgtcaccc gcgtacaaaa tgcgctgcaa agcgtaccgg
    19861 gcgtcactca ggcacgggta aacctagcgg agcgtactgc gctggtgatg ggcagtgcct
    19921 ccccacaaga tttagtgcag gcggtggaaa aagcgggcta cggcgcggaa gcgattgaag
    19981 atgacgcgaa acgccgcgaa cgccagcaag aaaccgccgt cgctacgatg aagcgcttcc
    20041 gctggcaggc aattgtcgca ctggcggtgg gtatcccggt gatggtctgg gggatgatcg
    20101 gcgataacat gatggtcacc gccgacaacc gcagcctgtg gttggttatc ggcctgataa
    20161 ccctggcggt gatggttttc gccggcggcc atttttaccg cagtgcatgg aaaagcctgc
    20221 tgaacggtgc agcgacgatg gatacgctgg tggcgctggg aacgggcgtg gcgtggctct
    20281 attcgatgag cgtcaacctg tggccgcagt ggttcccgat ggaagcgcga catctttact
    20341 atgaagccag tgcgatgatt atcggtctga tcaatctcgg ccatatgctg gaagcacgcg
    20401 cacgtcagcg ttcttctaag gcactggaga aattactcga tttaacccca ccgacggcgc
    20461 gcctggttac tgacgaaggt gaaaaaagcg tgcctctggc agaagtgcag ccaggtatgt
    20521 tgctgcgcct gacgaccggc gatcgcgtgc cggtagatgg cgagattacc cggggcgaag
    20581 catggctgga tgaagcgatg ctgacgggcg aaccaatccc gcagcaaaaa ggcgaaggcg
    20641 atagcgtcca tgccgggaca gtggtacagg acggcagtgt gctgtttcgt gccagtgcgg
    20701 ttggcagcca taccacgcta tcaaggatca ttcgcatggt gcgccaggcc cagagcagca
    20761 agccagaaat cggtcagctg gcggataaaa tctcagccgt atttgtgccg gtagtagtgg
    20821 ttattgcgct tgtcagtgcg gcaatctggt atttctttgg tccggcaccg cagattgtct
    20881 ataccctggt gattgccact acggtgctga ttatcgcctg cccgtgtgcg ctgggactgg
    20941 cgacgccgat gtcgattatt tccggcgtcg ggcgggctgc tgagtttggc gtgctggtgc
    21001 gggacgccga cgcgctgcaa cgcgccagta cactcgatac cgtcgtgttc gataaaaccg
    21061 ggacgctgac cgaagggaag ccgcaggttg tcgcagtgaa aacatttgct gatgttgacg
    21121 aagcgcaggc gctgcgtctg gcggcggcgc tggagcaagg ttccagccat ccattggcac
    21181 gagcgatcct tgataaagca ggcgatatgc agctaccgca ggtcaacggt ttccgcacat
    21241 tgcgcgggct gggcgtgagc ggtgaagcag aaggtcatgc gttattgctg ggtaatcagg
    21301 cgttgttaaa tgatcagcaa gtcgatacta aagcgattga agccgatatt tcagctcagg
    21361 catcgcaagg agcaacgcct gtgctgctgg cgattgacgg gaaagcagta gccctgctgg
    21421 ctgtacgcga tccgttgcgt agtgatagcg tggcggcgct gcaacgcctg cataaagcgg
    21481 gatatcgtct ggtgatgttg accggggata acccaaccac cgccaatgcg atcgccaaag
    21541 aagctggaat tgatgaagtg attgccggag tgctgccgga cggtaaagcc gaagcgatca
    21601 aacgtctgca aagtgaagga cgtcaggtgg caatggtggg cgacggcatt aacgacgcgc
    21661 cagcgctggc ccaggcggat gtcggcattg cgatgggtgg cggcagtgat gttgccattg
    21721 aaaccgcggc gattaccctg atgcgccata gcctgatggg cgttgcggat gcgctcgcga
    21781 tttcccgcgc aacgctgcgc aacatgaagc agaacctgct cggtgccttt atctacaaca
    21841 gcatcggtat tccggtcgcc gccggtattt tgtggccgtt tactggaaca ctgcttaacc
    21901 cggtagttgc cggagcggca atggcgctct cgtcgattac cgtagtgagt aacgccaacc
    21961 ggttgctgcg gtttaaaccg aaggaataag tacgtcattg cggatgcgct gctttagggc
    22021 ggcgcatttg gcatcgacgc atgactgccg gatgcggcgt aaacgcctta tccggcctac
    22081 ggttcaggca caggtttgta ggcctgataa gacgcgtaag cgtcgcatca ggcaatggtg
    22141 ctcggatgcc ggatgcggcg tgaacgcctt atccggccta cgagatgaag cgatgttttg
    22201 gccttacgcg accttttttg cacgctcttc acggcgtgcc aaatattcag ccactgtttc
    22261 aatccgtccc aattcttcct gggtgcgcat gttgttttca acttcccaaa gatcaactgc
    22321 cgcctggagg tttagcctaa aatcgactgt ggtatcaaaa actttcgcca gacggaatgc
    22381 catctcagtg gtgagtttac gattgttatt gatcagtgca ctgacgcgat tacgatgaac
    22441 atgcagcaac tctgctaact cattgatttt caaatcgagc ggttccagat attcatagag
    22501 aagaatatct ccgggtgtcg tcggttttct tgttgcctgt ttcatggttt ttaactgttg
    22561 cttccctgtg aatagcgatg caagaacata ctgaatcata caaacacatt aaacaagtcc
    22621 ccaactccat ttgcgccatc cctcaccgcg cgctagcatg atatttcaca aagggaggtc
    22681 gtatggatct gttgtaccgg gtaaaaacgc tttgggccgc gctgcgcggt aatcattaca
    22741 cctggcctgc catcgatatc accctccccg gcaatcgcca ttttcatctg attggcagta
    22801 ttcatatggg tagccacgat atggctcccc tgcccacccg tttgctcaaa aagctcaaaa
    22861 acgccgatgc gctgatcgtc gaggcggatg tttccaccag cgatacgcct tttgctaatt
    22921 tgcctgcctg cgaggcactg gaagagcgca ttagcgagga gcaactccaa aacctgcagc
    22981 acattagcca ggagatgggc atttctccct cactcttttc tacccaaccg ctgtggcaaa
    23041 tcgcgatggt tcttcaggcg acgcaggcac aaaaactggg gctgcgggca gaatacggta
    23101 tcgattacca gctattgcag gcggcgaagc aacaacataa acccgtgatt gaactggaag
    23161 gggctgaaaa ccagattgcc atgttgctcc agctccctga caaaggactg gcgctgctgg
    23221 acgatacgct gacccactgg cataccaacg cacggttgct gcaacaaatg atgagctggt
    23281 ggctgaatgc accgccgcaa aataatgata taacgctgcc caatacgttc agtcagtcgc
    23341 tgtacgatgt gctgatgcat cagcgaaatc tcgcctggcg ggataaatta cgcgccatgc
    23401 cgccggggcg atatgtggtc gcggtcggtg cactacacct gtatggagaa gggaatttgc
    23461 cgcaaatgtt gcgctaaaaa aatggccaat atcgctattg gcccgtcaaa gaggaatttc
    23521 atttttttat tattatgccg tcactttaag cgacggtgta actcgattgt cgctcaatct
    23581 caccatcctg ccaatactct taggcaagat gactcttttt tttggaccgc catcaggcgt
    23641 atgcttgctt cgtttttttg tttaaggcag gaaaattacc atgacacccg cagttaaatt
    23701 actcgaaaaa aacaagattt cgtttcaaat ccatacctac gagcacgatc cggctgaaac
    23761 caattttggc gatgaagtcg tcaaaaaatt aggtttgaat ccggatcagg tctacaaaac
    23821 gctgctggtg gcagtgaacg gtgatatgaa acaccttgcc gtggccgtta cgccggtcgc
    23881 aggtcaactg gatcttaaaa aagtagcaaa agcgctgggt gccaagaaag ttgagatggc
    23941 cgatccgatg gtcgcgcagc gttcgacggg atacctggtt ggggggatta gcccactggg
    24001 gcagaaaaaa cgtctgccaa cgattatcga cgcccccgca caagaatttg ccactattta
    24061 tgtttccggc ggcaagcgcg gactggatat cgaactggcg gcaggcgatc tggcaaagat
    24121 cctcgatgcc aaatttgctg atatcgcccg ccgcgattaa gcattgtgcc ggatgcaaac
    24181 atccggcact ttcggattac tgccagctca cctcaccttt cggttcataa acactcacat
    24241 ccagcggcga gcttttctgg atatacgctt tcagcacttc ggcatcaata aagccggtat
    24301 tcacatagcc cggattgtta tcaaggcgcg gatatccatc accgccggtg gcattgaagt
    24361 ttaatgtcgc catacggtaa gttttcgccg gatcgaccgg ttcgcctttg attttaaggt
    24421 cgttcagttt gccgtctttc gccacaaagc taacgttggc aaattgcggg taggcacctg
    24481 aatctggctt catctgcgcg acggcggtca ggtaatcaat cacctcttta ccggtcatgt
    24541 cggcatacac caccacattg ccgaatggct gcactttcag cacgttttta tagctgatat
    24601 cgcctgcttc gatagaatca cgaattccgc ctccgctcat caccgcaaag tcggcaccag
    24661 tgcgatccat ttgggctgcc agaatcaacc gccccatatt ggtctgtaca aaacgcactt
    24721 tgtcacgatc gccttccaga cgaccattgg tttcgcctat tttcacttcc agctgcgctt
    24781 tgcctttgtt ctggaacggt gataacagcg agatcatttg ctggttttca gcgatttcag
    24841 gagtgtaaag cacgcgctcg cttttcccgt cttcccaggt cactttcttc ttcaggttca
    24901 ccggaatcag ctggtagtta accattttca tttcgccatt acgaaactca aaatcagccc
    24961 gtcccacgta tttgccccac tcatgcgcct gcacaatcca gatgccgttt tgttgatctg
    25021 gtttgcatgg cgtacccggc acgtaatcga cctgtttttt gttttctgcc gccatgcaga
    25081 ccggatcttg cgagtgacca ccgacgatca tcgccagcga tccggcaggc agcgcgcgtg
    25141 ccatctccac atcgcccggt gcgttagagc cgtactcacc attatcgtaa tgccccatat
    25201 gggtcgccgc gataataatg tctggctttt ctgtctgttg cagctcctga atcaccagct
    25261 tcgcttcatc ggcgggctta cgaaattcga tatcagtgaa gtattccggg ttaccaattt
    25321 ttgctgtgtc atcggttgtc agcccaataa cggcaatttt cagatcctga cgcttaaaca
    25381 gcgcccacgg tttaaacagg cgctcgccag tacttttctg gtagatattc gcggaaagca
    25441 acgggaactt ggcccacttt tcctgctgac gtaatacggt gagcggatta tcaaattcat
    25501 gattaccgat cgccatcgcg tcatagccca ccagattcat accgcgaaaa tcaggttcgg
    25561 catcctgtaa gtcagactcg ggcacgccag tgttaatgtc gccaccggaa agtagcagca
    25621 cgctaccgcc ttcagccgca acctctttgc ggataccatc caccagcgtt ttttgcgccg
    25681 ccagaccata ttcgccatat tcattgcgcc aaaaatgccc atgatgatca ttggtatgca
    25741 gaactgtaat tttgtaggtt ttatcctgct catacgccag agcagtttca ctcgccagtg
    25801 taaatgtggt taacagcgct aacgccacgc cccgctgcaa taatttcata cttctctccc
    25861 tgacctgatt tcaacgtgtt gaaaaatagt atgacgcgac atcatagaca agtctgcgtc
    25921 cggtgaagat aaatctgcat ccagattaga aattctgtat tcggcgttga gacttccttc
    25981 agacgacgca tacaggtatg ttagtcgggt aataatcaca aataagatca ttctttacat
    26041 cgcaaccctg aaaaagtgac tttcctatgg caatgagtga acaaacccag cctgtggcgg
    26101 gcgcggctgc gtcaacgacc aaggcccgaa catcgtttgg tattttaggt gctatcagcc
    26161 tctcacatct gctgaacgac atgatccaat cgctgattct ggcgatttat ccgctgcttc
    26221 agtcagaatt ttctctgaca tttatgcaga ttggcatgat aaccctcacc ttccagctcg
    26281 cctcttcgct actgcaacca gtggtcggct actggaccga taaatatccg atgccgtggt
    26341 cgttgccaat tggcatgtgc tttaccttaa gtggtctggt gctgcttgcg ctggcgggca
    26401 gttttggcgc agttctgctg gcggcggcgc tggtcggtac cggttcatcg gtctttcatc
    26461 cggaatcttc tcgcgtagcc cgtatggctt ccggcgggcg gcatggccta gcgcaatcta
    26521 tctttcaggt cggcggcaac tttggcagtt ccctgggacc cttgctggcg gcggtgatta
    26581 tcgcgcctta tggcaaaggc aacgttgcct ggtttgtgct tgcggcactg ctggcgatcg
    26641 tggtgttggc gcaaattagc cgttggtact cggcacagca ccgaatgaat aaaggaaaac
    26701 ccaaagcgac gataatcaat ccactgccgc gcaacaaagt ggtactggca gtcagcattc
    26761 tgttaatcct cattttctcg aaatatttct atatggcgag catcagcagc tattacacct
    26821 tttatctgat gcaaaaattc ggattatcta tccagaatgc ccagcttcat ctgtttgcct
    26881 tcctgtttgc cgttgcggca ggtacggtga tcggcgggcc tgtaggggat aaaattggac
    26941 ggaaatatgt gatttggggc tctatcctcg gcgttgcgcc gtttacgctg attttaccct
    27001 acgcccgcct gcactggacg ggggttttaa cggtgattat tggatttatc ctcgcttcgg
    27061 cattctctgc cattctggtc tacgctcagg agctacttcc gggacgtatc ggtatggttt
    27121 ctggactctt tttcggtttt gctttcggca tgggaggtct gggagcggca gttctggggc
    27181 ttatcgccga tcacaccagc atcgagttag tctataaaat ctgtgctttc ctgccactat
    27241 tggggatgtt gaccatattc ctgcctgata accggcataa agactgattt cattgcagcc
    27301 aaaggcaaac tttggctgca tcgttttaca gtcgccataa gcctttcctc tgttaaaccg
    27361 ccttctgttt tccgatccgt gctttttttg cagctaattc catttttctg caaaattcaa
    27421 caattattca taaacatcat caatgaaatt gtcataaact attacctgta attttggttt
    27481 tcccggccaa aaatggacaa taaccaccag gaaaaggaga cggaatgcat cacgccaccc
    27541 cgcttatcac caccattgtt ggcggccttg tgctcgcctt tatcctcggc atgctggcca
    27601 ataaactacg tatttctcct ctggtgggat atctgttagc gggtgtgctg gcaggaccat
    27661 tcactccggg ctttgttgcc gataccaagc ttgcgccgga actggctgaa ctgggcgtta
    27721 ttttgctgat gttcggcgtc ggtttgcatt tttcgctcaa ggatttgatg gcggtaaagt
    27781 ccatcgccat tcccggcgcg atcgcccaga tagccgtggc gacgctgctg ggtatggcgc
    27841 tctccgccgt gctgggctgg tcgttaatga ccggtatcgt gttcggctta tgtctttcca
    27901 ccgccagtac cgtggtgtta ctgcgcgcac ttgaagaacg gcaattaata gacagtcagc
    27961 gtgggcaaat cgccatcggt tggttgattg tggaagacct ggtgatggtt ctgacgctgg
    28021 tgttgctgcc cgcagtggca ggaatgatgg aacagggcga tgtgggcttt gccactcttg
    28081 cagtcgatat ggggatcacc atcggcaagg tgatcgcatt tatcgccatt atgatgctgg
    28141 taggtcgccg tctggtgccg tggattatgg cacgcagcgc ggcaaccggt tctcgcgagc
    28201 tgtttaccct gtcagtgctg gcgctggcgt tagggattgc ctttggtgcg gtagagctgt
    28261 ttgatgtctc ctttgcactc ggtgcgttct ttgccgggat ggtactgaac gagtctgaat
    28321 tgagtcaccg tgccgcccac gatacgttgc cattgcgcga cgcgtttgcg gtgctgtttt
    28381 ttgtctcagt cgggatgttg tttgatccgt taattctgat tcagcaaccg ctggcagtgc
    28441 tggcgacgct ggcaattatt ctgtttggta aatcggtcgc ggccctgttc cttgtccgtc
    28501 tgtttggtca ctcccaacgt acggcattaa ccatcgccgc cagcctggcg cagattggtg
    28561 agttcgcgtt tatcctggcg ggactgggaa tggcattgaa tttactgccg caggccggac
    28621 aaaacctggt actggcaggg gcgatcctgt cgattatgct caacccggta ctgttcgcac
    28681 tactggagaa atatctggcg aagaccgaaa cgctggaaga gcagacgctg gaagaggcaa
    28741 tcgaagaaga gaaacagatc ccagtggata tttgcaacca tgcgctattg gtgggttacg
    28801 gtcgtgtagg cagcctgctg ggggagaaat tgctcgcctc tgatattccg ctggtggtga
    28861 ttgagacgtc acgaacccgt gttgatgagc tgcgagagcg tggggtccgc gctgtattgg
    28921 gcaatgcagc gaacgaagaa attatgcaac tggcacatct ggaatgtgca aaatggctga
    28981 tcctgacgat ccccaacggt tatgaagcgg gtgagattgt ggcgtctgcc cgcgcgaaaa
    29041 acccggatat tgagattatc gcccgcgccc attatgacga tgaagtgacg tatatcaccg
    29101 aacgtggcgc gaatcaggta gtgatgggtg aacgtgaaat tgcccgtact atgctggaac
    29161 tgctggaaac gccaccggcg ggtgaagtgg tgacggggta aacggtggtt gccggatgta
    29221 aaatatgaaa aatatgtagg catgataaga cgcgccagcg tcgcatcagg catcgagagc
    29281 caaatgccag atgcggcgtg aacgccttat ctggcctacg aaccgacgat aacttaacga
    29341 tcccagtaag actcttccag gctgtcttca cgctccggca agccgcgcgt taaacgaggt
    29401 gaatgctggt tcagtacctg atagctcaca cggttagcat atttacacac ctgcgctaac
    29461 gatgaataag ttaaccaggt gaatttatgt ttgctggagt ttggtatgtt gctacgatgg
    29521 tagctgttgg cggtaatgtc atgcagcaac gctgccaatg cgccatcccc cgctccatta
    29581 gtgttcatga ttttttccgg cccgcccatg tacggcgcaa tgtgcgaata gacacgcagc
    29641 ggattctggc aatccttgtg gcgcatggcg cggctaaact catactggtt gaattccgct
    29701 atagcgcccg gcagcagcgg atgctgggtt ttacgtttcg cttcgtcttc ggtaaagccc
    29761 gccatataca agccgattgg cccggcggtg cacagcacca gatctaccca gtccagcgcc
    29821 ttgtcagatg ccaacaacgg atcgctttct ccggtcaacg cttcggcttc atcttcgttc
    29881 atcgcaagga tagaaacgtg gtctttgagg aattgctgcc accactgcgg attctcggca
    29941 atgacaaact tggtgcccag cgtcagcacc accggtacgt tatatttctt cgcgtactca
    30001 atggctttca tggttgcttc cggcatgggt tcacccggct tgcaacgcac cagatatgag
    30061 gtgagaacca gtgccgaggc tccggcaatc acatcttccg gaatgctttc agcccgcagc
    30121 tggttcatgt ggcttggact gatagcaaag gtacgttccc cggactcgcc aatcagcgta
    30181 aagcaacgac caatcgggcc atccacgcct tgtagatagt taagatcggt acggctggaa
    30241 gtgttacaca ggtaacgata ggcataactg ccaatttcaa tattgctgca catgacgccc
    30301 agcagcaccg aacggtcgtc cgcgagcacc gagtagttgt gcatggtgtt accaatggtg
    30361 ccacccgcaa actgatgggt aatcagattt ttctgtttta gctcctgata aagcgcttcg
    30421 gctacgtcat cctcaatcac cagtgaatgc ccggcgctta atccataacg ctcaatgaat
    30481 tcatcatcca ctttcgcttc aatatcgacc agcgtctggt caatacctac gacccaggcg
    30541 gcgctggttt cgttttctgg ctggaattgc tgaagcagcg gatcgcgtgc atttacaggg
    30601 aagtaatgtt tggatttacg tttaccggga aatttcatgg ttgtttttac gggtggttaa
    30661 ctgagcggcg aatgttaaca caatctgacg ggagcgcgcg agacgcagcc agtttcaata
    30721 tcagcaggcg ggatcgcatt ttcatggcag tggttcctta caatgacgta atttgaaagg
    30781 agtttttgta tgaagccgga aaacaaacta cctgttctgg accttatttc tgctgaaatg
    30841 aagaccgttg tgaatactct tcagccggat ttaccgtcct ggcccgcaac gggaacgatt
    30901 gctgagcaac gacagtatta cacgcttgag cgccgattct ggaatgcggg cgctccagaa
    30961 atggcaacca gagcttacat ggttccaaca aaatatgggc aggtggaaac acgtctcttt
    31021 tgtccgcagc cagatagccc ggcgacgcta ttttatttgc atggaggcgg ttttattctc
    31081 ggcaatctcg atacccacga tcgcatcatg cgcctgctgg caagctacag ccaatgtacg
    31141 gtgattggta ttgattacac cctttcacct gaagcgcgtt ttccacaagc gatagaggaa
    31201 attgtggctg cttgttgtta tttccaccag caggcggagg attatcaaat caatatgtcc
    31261 cgcattggct ttgccggtga ttccgcaggt gccatgctgg cgctcgccag tgcgttgtgg
    31321 ttgcgtgata aacagatcga ttgcggtaaa attgcgggtg ttttgctgtg gtatgggctt
    31381 tacggattac gggattccgt gactcgtcgt ctgttgggcg gtgtctggga tggcttaacg
    31441 caacaggatt tgcagatgta tgaagaggcg tatttaagca acgacgcgga ccgcgagtca
    31501 ccgtattact gtctgtttaa taatgatctc actcgcgaag ttccgccctg ttttattgcc
    31561 ggggcggagt tcgatccgct gctggatgac agccgtctgc tttaccagac gttagcggcg
    31621 catcagcagc cctgtgagtt caaactctac ccaggtacgc tgcatgcctt tttgcattat
    31681 tcacggatga tgaaaaccgc cgacgaggct cttcgcgacg gcgctcagtt ctttaccgct
    31741 cagctttagc gatacgcggc aacaagatta gccatcattt cgatatgttc cggcgtggca
    31801 ttaagcgctg gaatatattc atattttttc ccgcccgcac cgaggaagac ctcacggttt
    31861 tgctcggcaa tctcttccag cgtctccaga caatccgcag caaagcccgg gcacatcacc
    31921 tgtatatgac ctacgccttt ttctccgagc attttcagcg cttcgtcggt ataaggcatc
    31981 agccagggtt cccggccaaa gcgcgactga aaggtcatca tcactttttc cggtgccatc
    32041 cccagtgcgg aagccagttc gcgagtcgtt gtgcggcaac gttgcgggta atcatcgcct
    32101 tcatctgcat aacgctgggg aatgccatga taagagagca gcagcagatc tggttcgcca
    32161 tgtttggcaa aagaagcgcg tacgctgttc gccagtgcat taatgtaatc gtggttatcg
    32221 gcgtaatcac gaataaacga tatccccgga atgctacgtt tgcgcgccag aatgcgtgcc
    32281 agttcatccc ataccgcacc gaccgtagaa caggagaatt gcggataaag cggcagcacc
    32341 acaatatgat ctacatgctc tgccaggagt tcatcgacgg cgctttccag tgatggcgag
    32401 ccgtagctca tgcccagcgc cactggcgta tccggtaaac gttgtgccaa cgcctgctgt
    32461 tgctggcggc tgtaaaccat cagcggcgag ccgtcttcca tccagacaga ggcgtacagc
    32521 ttcgccacac gcggcgagcg cagcggcaaa atcacgccgc gcagcaacgg ccaccataac
    32581 aaccgtgagg tatcaaccac gcgtctgtcg cttaaaaatt gtttcagata gcgttttacc
    32641 gcttcaggtg tgggggcatc gggcgtaccc aggtttgcca gcaggatacc ggttttagtc
    32701 tgacgcatta ccgcctctta tcgattcaac ttgttgataa ttgtagcgga aaagtgagga
    32761 agaagaacta attgctgtga gaggtattgc ctgaggtgga tgagcagacc tgcccgaaag
    32821 cagatctgct gcggcttgat tagccgagga ttttttccag atcagcgcga acttcagcaa
    32881 ccggcttggt accgtcaact ttcgcgtatt tggtgttacc cgcttccgct tctttggagt
    32941 agtagccgat cagcggtgca gtcatctgat ggtattcaac cagacgttta cgcacggttt
    33001 cttcctgatc gtctttacgg gtagtcagtt cttcaccggt aacgtcgtct ttgccttcta
    33061 ctttcggcgg attgaattta acgtgataaa cacgaccaga cggcgcatga acgcggcggc
    33121 ctacgatacg atcaacaatc agttcgtccg gtacgtcgaa ttccagaacg taatcaacat
    33181 tgatgcccgc ttctttcatc gcgtctgcct gcggaatggt acgcgggaag ccgtccaaca
    33241 ggaaaccatt acggcagtct tcctgagcaa tgcgctcttt aaccagcgcg atcaccagtt
    33301 cgtcggtgac cagtttgcca gcatccataa tgtcttttgc ttgtttaccc agctcggagc
    33361 cagatttgac cgcagcacgc agcatatcgc cagtggagat ttgcggaata ccatatttct
    33421 ccatgatgaa ctgagcctga gtccctttcc ccgcgcccgg agcgccaagc agaatgatac
    33481 gcattgcgaa aatcccctta aaatgtgtcg atttttttga aaaagcgata aacgatacca
    33541 ccaacaggcg attgcctcaa gaaaggcacc tgaggctgaa acggttaatg gcgagataat
    33601 ttgcggaaag tgcagatgat gaaaagaaaa atgccggatg acacaaaggt catccggcat
    33661 gacatcagga aaccagcagc tggttcatac gacgaataaa caggttcgga tcttccagcg
    33721 tgccgcgttc tgccagcagc gcctgatcca gcagcagttc tacccactcg ctgaacttgg
    33781 cttcatcttc agtatctgcc gcacgtttca ccagtacgtg atccgggttc agttcgaaga
    33841 tgtatttcac ttctggcact ttctggcccg ccgcagcgaa cagtttcgcc atctgagtgc
    33901 tcatttcgtc cgcgtcggtc gaaacgatcg ctggcgtatc ggtcagacgg tgagtcagac
    33961 ggacatcttt cacgcgctcg ccgagcagag ctttcacgcg gtcgatgaac ggagtcagtg
    34021 ctttctccgc ttctttcgcg ctctcatcaa cttcgtcagc cagtttttcc agcgactcgt
    34081 caactttaga caccgactgg aacggtttac cgtcgaactc agtcagatag ttcatcatcc
    34141 actcatcgat gcggtcggaa agcagcagaa cttcgatgcc tttcttacgc agcagttcca
    34201 ggtgcgggct gctcttcgct gccgcatagc tgtctgcagt gatgtagtag attttctcct
    34261 gcccttcttt catgcgggaa acgtagtctt ccagagacac ggtctgcgca gaagaatcgg
    34321 tataagtaga agcaaaacgc agcagtttgg cgatcgcttc ctggttagcg aaatcttccg
    34381 ccggaccttc tttcagtacc aggccaaact gttgccagaa ggtctggtat ttttccgcgt
    34441 cgtctttcgc cagtttttcc agcatttgca gcacacgctt ggtcagcgca ttgcgcaggt
    34501 tacgcgttac cgtgctgtcc tggaggattt cacgggaaac gttcagcggc agatcgctgg
    34561 agtcaatcag accacgcacg aagcgcagat agttcggcat gaactgttca gcgtcgtcca
    34621 tgataaacac gcgttgaacg tacagtttca ggccgtgttt atgatcacgg ttccacatat
    34681 cccacggagc ctgggacggg atgtacagca ggctggtgta ctcctgctta ccttcaacgc
    34741 ggttgtggct ccaggtcagc ggatcgttaa agtcgtgggc gatgtgtttg tagaactctt
    34801 tatactcttc atcggtgatt tccgacttgt tacgagtcca cagcgcctgc gctttgttga
    34861 tttttgccca ggagataacg gtttcgccat ctttctcttc gcgtttttcg atctctaccg
    34921 gcagcgcgat atggtcggag tatttgctga tgatggaacg cacgcgccag tcatcgagga
    34981 actcgtcttc gccttcacgc agatgcaggg tgatttcagt accacgatct tctttggtga
    35041 tgtcggcaac ggtatattca ccttcgccag ccgattccca gaagacgccg ttttctggtt
    35101 tttcgcctgc cgcgcgagta cgcacggtca ctttgtcggc cacgataaac gcagagtaga
    35161 aaccaacacc aaactgaccg atcagctggc tgtctttcgc ctggtcagaa cccagggatt
    35221 cgaggaatga tttggtaccg gatttagcga tagtccccag atggtcaatc acttcgtcgc
    35281 gggtcatccc cacgccgtta tcggagatgg tcagcgtgcg cttgtcttta tcgaaagaga
    35341 cacgaacgcg cagttcgcca tcaccttcgt acaggtccgg gttagatagc gcacggaaac
    35401 gcagcttgtc cgccgcatcg gaggcgttag agataagctc acgcaggaag atttctttat
    35461 tggaatagag agaatggatc atcaggtgca gaagctgttt cacttctgac tgaaaaccac
    35521 gagtttcttg tcctttcatg taggtctacc tcaataatgc cattttaagg ttaaaaacag
    35581 gatgagggag agatggggac aagggagaat aatttcaagc gagagcaggt gctcctgctc
    35641 tcgtttgctt aaaaacgaat cttatgacgc ccggcgaggg agtgtgacag cgtggtgcca
    35701 tcgaccattt ccagttcgcc gccaaccggt acgccatgag cgattcggct ggcttccacg
    35761 tcatattgcg cgcaaagctc ggcaatgtag ttagcggtag cttcaccttc aaccgtggga
    35821 ttggtggcga ggatcacttc agtgattttt tcctctgcca gacgctgttc cagacgatca
    35881 agcccgatat catccggacc gatgccgtcc agcggtgaca gatgccccat caacacaaaa
    35941 taacgaccag aaaactgccc cgtctgctca atggcgtaga tgtccgccgg actctccacc
    36001 acacagattt gaccgttttc ctgacgacgc ggattcgaac agatgttaca gacttcctgt
    36061 tcggtgaaag tgcggcaatc ggcgcagtgg ccgatttccg acatcgcccg ggtgagcgcc
    36121 tgcgccagac gcatcccgcc gctacgatcg cgctgaagca gcgtgaacgc catacgctgc
    36181 gccgacttcg ggccaacgcc cggcagacag cgcagtgctt ccataagctg tgttaacagc
    36241 gggctggttt gcatcagaac ggcatcttaa agccaggcgg cagctgcatt ccggaggata
    36301 cagaggccat tttttctttc tgcgtttctt caatacgacg tgctgcgtcg ttgaatgctg
    36361 cagccaccaa gtcttccagc atctctttgt cgtcttccag caggctcggg tcgatctcta
    36421 cgcgacggca gttgtgtgca ccgttgatgt tcacttttac cagacctgcg ccagattcgc
    36481 cggtaacttc cagctgcgcg atctcttcct gcattttctg cattttttct tgcatctgct
    36541 gggcttgctt catcaggtta cccagaccgc ctttaccaaa cataggtttc tctctcaatc
    36601 acgttaagga tgacgaacgt aagctgtgct tacgatcaaa tggggcggat actttcttca
    36661 tccagctccg catcgaaaaa ccgacgcagg gtctgaatat tattatccgc aataatggac
    36721 tcgcgcgcct gcgcaagttt ttcttcgtat atcgcctgac gccactccag cggcgtacgc
    36781 accgcgggat tatcatcttc aacgatagtc agttcaaccg ttgaaccttt taacatgctc
    36841 aacgcttcag ccagtttttg ctgtgcaccg cggttgttca aatgccgctg agaggagcgc
    36901 aaatgcagac atactgcgtt gtcgctctcc tctttccagg catttaacgc cacctgttcg
    36961 accagttttg gtagcgaaag ttggctcacc tgtgccgccc acgggtcgcg ctcaatggct
    37021 tctgccgcta gcttcgccgc cagttccggc gttttttcat gttccagcgc ttttttcagc
    37081 gccttcggcg tggcgaccac ttctttttgc tgcatcaccg gagtggtcgc cttccagcga
    37141 tacgcttctt ttttggctgg cgctttttcc agcgccgatg gcaccggacg cgcctgaacg
    37201 cgatcggtga ccgaagccag tctttccagc gcagcgttat tcaccggccg cgcgcgggta
    37261 gcggctgccg gttcactctt ttttgctttg gttgctccct gcacgcgctg caactgctgg
    37321 cgcgccgcca gcacctggct ggtggtttcc gggagcggta cagtcggtgc ctgctgcggc
    37381 gctgattgcg gcggcaccag ggttggcgtc attactgccg ttggcgcgac gggtgcaaag
    37441 gactggcgtg gcacttctgg ctcaggcagc ggcatacgcg gatggaacgc cagcgcgcgc
    37501 agcagcgtca tctcaacgcc catgcggcgg tccggcgcat acggtaattc tttgcgacca
    37561 atcaacagcg tctgatagta aagctgaata tccgtcggcg gtatggtgcg cgccagttca
    37621 cgcatccgca gctcgatggc ggccatgtcg ttgccaagtg cagcaggcga aagttgtacc
    37681 atcgcaatac gatgcaacaa gccgagcatt tccaccagca acgcttccca ctcgatacca
    37741 cgggcagcgg cttcattaat cagtgccatt acgcgctcgc cgttgccctc gaccatcgct
    37801 tcaaccagcg acagcgcctg atcgtcgtca agcgtaccca gcatcgcact gaccgcctgg
    37861 gttgaaacct ggccgtcacc gctggcaatc gcctggtcgg tcagacttaa ggcatctcgc
    37921 aggctgcctt cagcggcgcg cgccaacaat tgcagcgccc gcggctcgtg agcgatatgt
    37981 tcttcattga ggatgtgctc aagctgatgg cgaatttgct cgacatccag cgccttgaga
    38041 tgaaattgca gacagcgtga caaaatcgtc accggcaatt tctgtggatc ggtcgtcgcc
    38101 agcagaaact taacgtgctc cggcggctct tcaagggttt ttaacagtgc gttaaagctg
    38161 tggcgcgaca gcatatgcac ttcgtcgatc agataaactt tgaaacgacc acgcgccgga
    38221 gcgtactgga cgttatccag caggtcgcgg gtatcttcaa ctttggtgcg cgaggcggcg
    38281 tcgatttcaa tcagatcgac aaagcgcccc tgctcgattt cacgacagtt atcgcacacg
    38341 ccgcacggcg tcgcggtaat gccggtttcg cagtttagcc ccttcgccag cagtcgggcg
    38401 atagaggttt ttccgacgcc acgggtgccg gaaaaaagat aagcatgatg aatacgccct
    38461 aacgacaagc cgttcgccag tgcggtcagc acatgttcct ggccgacgac gtcagcaaag
    38521 gtttgtgggc gccattttcg ggctaaaacc tgataactca ttggcaggct ctgaaacgct
    38581 ggaaggtgga ttcacgaagg ggtaatgcta acacagcccc gtcagaacgg cgaggctgaa
    38641 tgcacatctt gtgctgtccg tagcgtgggc agcacaagac tggcgataat taatggcccg
    38701 ggaacgggac aaggctgtag ctggtaatgc cctgtttttc gagacgctgt tcgccgccga
    38761 gatcgaacag gttgataatg aacgcagcgt cagccacttc accacccaga cgacggatca
    38821 gtttaacggt cgcttcgata gtgccgccgg ttgccagcag atcgtccacc accagaactt
    38881 tgtcgcccgg tttgatggca tcaacgtgga tttccagctg atcggtgccg tattccaggt
    38941 cgtaattttc actgatggtt tcacgcggca gtttgcccgg tttacggacc ggtacaaagc
    39001 caacgcccag acccagagct accggagcgc caaacaagaa gccacgcgct tcggtgccga
    39061 caactttggt aatgcccgca tttttgtaac gctcaaccag caagtcgatg ctgagagcgt
    39121 aagctttcgg gtcttccagt aagctggtga catcgcggaa aagaatgccg ggttttgggt
    39181 agtcctgaat gcttttgatg ctatttttga gatactcaag ctgctgtgca gtcgcggtca
    39241 taagtgtatg cctgcttgtt acggtggtac tcacggcgcg ttttaaacgt atcaaaagta
    39301 actgttgttt aacctttgac cgcctgcgcc tgtgctcgaa aacgccagaa tgtactggct
    39361 gtacgcaata attgcaactg cgattgttgt gcttcagtgc ttttcttgct tttcatcaat
    39421 caccggaatt cgccacatat agaaaagcaa acaggcgaga attaccagca acatgatgcg
    39481 cacccatggc atctggacga accacagaga aatggcaaac gtgagcaaaa taagcaaaat
    39541 cgcccgcggt ttgacgccgc gcggcatcgc atgatgtttc tgccagaaac gtagatagct
    39601 gccaaaccat gagcggtaca gcaaccaggc gtgaaagcgc ggggaagaac gggcaaagca
    39661 ccaggccgcc agcaggataa acggcgtcgt cggtaatacc ggtaatacca cgcccagcgt
    39721 acccagcact accgccagcc agccaatgat gattaaaatg attcgttgca tattgagtgt
    39781 tgtcagctta cagagtggct actttagcat aacaattatc attttcattg aggtcttatc
    39841 gtgaaaaccg ccctgctgct ggaaaaactg gaaggacagc tcgctacgct gcgtcagcgt
    39901 tgtgccccgg tgtcacagtt cgccacgcta agtgctcgtt tcgacaggca tctttttcag
    39961 actcgtgcga caacactaca ggcttgtctc gacgaggcgg gcgataatct ggctgcgctt
    40021 cgtcatgcag ttgagcagca acagctgccg caagtggcct ggctggcgga acatctggcg
    40081 gcacaactgg aagccatcgc gcgtgaagcc tccgcctggt cattgcgcga gtgggacagt
    40141 gcgccaccga aaattgcccg ctggcagcgt aaacgtattc agcatcagga ttttgagcgg
    40201 cggctacgtg agatggttgc cgaacgcaga gcccgcctgg cgcgggtgac cgatctcgtg
    40261 gaacagcaaa cgctgcatcg tgaagtggaa gcctatgaag cgcgtctggc acgctgccgc
    40321 catgcgctgg aaaaaatcga aaacaggcta gcgcgcttaa cccgctagca atggagagag
    40381 tatgtcactt gaaaatgccc ctgacgatgt caaactggct gtcgatttga ttgtgctgct
    40441 ggaggaaaat cagatccctg ccagtaccgt gctgcgcgcg ctggatattg taaagcgtga
    40501 ttatgaaaag aaattaacgc acgatgatga ggcggaaaag tgagaaataa atggggccgc
    40561 taaatgtccc ctaagattgc gggcaaacgc aaaactgcct gatgcgctac gcttatcagg
    40621 cctacaccgc tcctgcaata tattgaattc ttgcggtttc gtaggccgga taaggcggtc
    40681 acgccgcatc cggcatgaac aacgcgcact ttgtcagtaa tctcggggca actaaatgtc
    40741 gccccctttg gttattaccc aaccgctggt gtcggatcgt cgcctttgta gtcgcgtttt
    40801 acttccgtca cctcatcgcc cttctcgttg tgcagatgca cttcaagctg gttaaaggca
    40861 atgttgatgt cgttttcacg gcacagctga tcgatagtac ggttcagctc atcgacagta
    40921 cggctacggt cacgcagttc acgcacatac agacgcagct catgatccaa cgtgctggca
    40981 ccaaatgccg taaagaagac ttccggcatt ggttcgtgca tcacccttgg gtgctcagtc
    41041 gccgccttca gtaacacttt acgcactttt tccagatcgg agccataggc cacgccgaga
    41101 cggatcacca gacgcgtagt agtgtcagtc aacgaccagt tgatcagacg ctcggtaaca
    41161 aacgctttgt tcgggatgat cacttctttg cgatcgaaat cggtaatcgt tgtcgcacga
    41221 atacggatct tacttaccgt ccccgagaag ctaccaatgg ttaccgtatc gccaatacgc
    41281 accggacgtt cgaacagaat gatcaaaccg gagacgaagt taccgaaaat ttcttgtaaa
    41341 ccaaaaccaa gacctacgga taatgctgcg gccagccact ggagtttatc ccaagagacg
    41401 cccagcgatc cgaacaccgt catcgcacca acggcaataa tgatgtagtt aaggatggta
    41461 gtaatggcat acgacgcgcc ctggcgcata ttcagtcgcg agagcaccag cacttccagt
    41521 aaaccaggca ggttgcgaat caacgcccag gccaccattg aggcgataat cgcaaacaac
    41581 agactgccca tggtgacgtt tttcaccacc gcagcgccag cttcagtgcc gttgtaatgc
    41641 cagagcgtga tgctgtcgag atagctgaac acggtgatca aatcggacca aattgcccag
    41701 aacatgacac cgaacagcgc aaacatcagc aacatggtaa tacgcagcgt ctgctggtta
    41761 acttgctcca gtgcaatggt gggttcttcc ggcggttcgg caccttctgc gccctctttc
    41821 accagattct gccgacgcgc cagcgcacga cgccaggcga tacgccgcgc cgctacgctt
    41881 aagccgcgca ataccgtctg gtagagcaag ttccagatga tcaccagata aacggtttca
    41941 atccaacgcc cggagaggcg cagcgtggtg tagaaataac ccgtggcagt cagcaccatc
    42001 aatgcaatcg ggataatcga cagcacggta atggtgacca gtcgcatggt gtgcgactct
    42061 ttatcacgcc agctttcgcg gcacattggc cataccagga aggcaatcag cagcaggttg
    42121 aagaaaatca tcgcctgccc cagcacatca tccatcagat gcagcgggga aagttctgcc
    42181 accacagacc agaaatggat aggcagcaac gcgagactga tgcggacaat ttgccgacgc
    42241 cagtggctgg tctgctgctc cggcatgccg aagtgacgca cggcaacgcc atttttctcc
    42301 agtaccttcc agcacaggcc aaacaccagc cagaaaatcg ccagtttttt actgaacgac
    42361 catagcagtt cgctgatgtt gagctgcatg gtcaacagaa tcaggccaac cgcgagaata
    42421 atcaggcaca ccggcagcgc gcggatcagg tcgataagga tcgcttttgg tgtgttgagc
    42481 tggctatcgt tacgcaggga acccacagcg gaagccagtt tctgttgata cgctttcagc
    42541 cagcccagac gccagtggat cagcccggca atcaacagca gcggcaaacc ggcgaggaaa
    42601 gcgataaaaa cggcaggcca ggctttttcc cagttcaccg tgattttcat cgacttaaat
    42661 tcatctttca ggctttgcgg gaacgctttg atccagtccc agtccattgg acggttactg
    42721 ttcacccaaa agatttgctg agtcaggatg gatttcaggt ttttcgatac gctcattaac
    42781 tgctgctggt tgatttgcag gttaatggcc atcatcagct ggttacccaa ctgtttgttg
    42841 agttgatcca gcaattcgcg acgcatatca accacttgca ataacgcatc gtgaacttcg
    42901 ctgttgactt cgttggtgtg accttcttcc agtttgttga cgaacgcatc gctctggaag
    42961 agtgcatcac gctgctggtt aacttcaaac tgttcgagac gcaaatccgc gatgcggttg
    43021 gtcatgtttt ccagttcatc cgccgagggc agcgtttgtt gttgctggta aaggatacga
    43081 gacaacagca ggctgccctt caggacggca atctgctctt taatattgcg ttccgattgc
    43141 agcgcccgct ccagccagtt tttgacttta atgttttgct gcatcaactg attaccgttt
    43201 tcagtcgcgg taatcagacg ctgacttaac tgctggttaa tttccagttc ctgtttcacc
    43261 agcggattag cctgaatacg cgcggcttca tccggggaga cggcttcctg cgccgttttt
    43321 tcagttaaag tcaggcgctt gctgtttacc gcttcttgca acagctgcaa ctggtgctcc
    43381 agacgggcgc tgttcgccgt cacgtaatca cgttgctttt gcaaggtatc ctgcaagacg
    43441 gtgttccctt ccaggctttt acgctgctgg tcaatctccg cattcagcaa cgcctgctga
    43501 gcctgcatta acactttctg gctgggacgt aaggcagtct cgccgacatc agtcccatcc
    43561 agacgactgc gaatttgttg cagctgctgc gaagcgttat acatcgcatt ttgcacgcgt
    43621 tcgggctgcg tctgtaacga aaccagctgg ctgttataag acgccagatc gttttgtgcg
    43681 ttttgcaaat cgtccagcgc ctgggcaacg cgagtttcca gctggcgcaa cgaaagcgta
    43741 ctcagaattt tgcgcgtttc ttcgtcgtta tcgacatcgc taagtgctgt taacgccgcg
    43801 gtcgcctggc gcattttttc cggcgcttca gcgacttttt gccgtagctg aactgtctct
    43861 tcttttatgc gatcgatttt atcgagggtg gctaatgtat ctgtcagatc ctgctgcacc
    43921 agtttgtcct gagcagaaag atctttttgt ttatttagtg agtcaagttg cgcctgcagg
    43981 tccgcttttg tcggcagatc accattcgat gacgcccgcg caaacgccgt gttctggcat
    44041 aacaagacaa aaacaaaagc aataaatgct gaaaaaacaa aatgccgtga tcgtttgtaa
    44101 tactggaaca tagtcatgat gaatgaaggt ttctgaacct gaagaacgac ctgaaaaagt
    44161 caaaccgcaa gaatatcacg acgcagtgaa ccagaatagc aacgacgaaa atgtccagga
    44221 aaaatcctgg agtcagattc agggttattc gttagtggca ggattacgaa gcgtggggca
    44281 caggagatac atctccagta agatggcgac gtaatcgcgg gcttcttttt taagatcaaa
    44341 agattgcggg gcaaagagcc agttttccat caggccggaa atatagccgc gcataataat
    44401 tgctgcccga cgcgtcatta aatccgcagg caacattttc gcttcaatac aatgttttaa
    44461 cgtttgttct atacggtcat aactttccag acagagatta cgttgtgcct gttgcacaac
    44521 agccatttct ccgacaaatt cgcatttgtg gaatataatc tccatcaata atcgacgtcg
    44581 ttcttctgtc accgtggatt caagaacatg aattaatatc tctcttaata ctgagagtgg
    44641 atcgccaggg aattttgcct gatactcaag ctctagttca ccaatattgg attctgacag
    44701 ttcccagatc tcactgaaca aatccgactt gtctttaaaa tgccagtaga ttgcaccgcg
    44761 cgtaacgcca gctgcttttg caatctcgcc cagcgaggtg gatgataccc cctgctgtga
    44821 gaaaagacgt agagccacat cgaggatgtg ttggcgcgtt tcttgcgctt cttgtttggt
    44881 ttttcgtgcc atatgttcgt gaatttacag gcgttagatt tacatacatt tgtgaatgta
    44941 tgtaccatag cacgacgata atataaacgc agcaatgggt ttattaactt ttgaccattg
    45001 accaatttga aatcggacac tcgaggttta catatgaaca aaaacagagg gtttacgcct
    45061 ctggcggtcg ttctgatgct ctcaggcagc ttagccctaa caggatgtga cgacaaacag
    45121 gcccaacaag gtggccagca gatgcccgcc gttggcgtag taacagtcaa aactgaacct
    45181 ctgcagatca caaccgagct tccgggtcgc accagtgcct accggatcgc agaagttcgt
    45241 cctcaagtta gcgggattat cctgaagcgt aatttcaaag aaggtagcga catcgaagct
    45301 ggtgtctctc tctatcagat tgatcctgcg acctatcagg cgacatacga cagtgcgaaa
    45361 ggtgatctgg cgaaagccca ggctgcagcc aatatcgcgc aattgacggt gaatcgttat
    45421 cagaaactgc tcggtactca gtacatcagt aagcaagagt acgatcaggc tctggctgat
    45481 gcgcaacagg cgaatgctgc ggtaactgcg gcgaaagctg ccgttgaaac tgcgcggatc
    45541 aatctggctt acaccaaagt cacctctccg attagcggtc gcattggtaa gtcgaacgtg
    45601 acggaaggcg cattggtaca gaacggtcag gcgactgcgc tggcaaccgt gcagcaactt
    45661 gatccgatct acgttgatgt gacccagtcc agcaacgact tcctgcgcct gaaacaggaa
    45721 ctggcgaatg gcacgctgaa acaagagaac ggcaaagcca aagtgtcgct gatcaccagt
    45781 gacggcatta agttcccgca ggacggtacg ctggaattct ctgacgttac cgttgatcag
    45841 accactgggt ctatcaccct acgcgctatc ttcccgaacc cggatcacac tctgctgccg
    45901 ggtatgttcg tgcgcgcacg tctggaagaa gggcttaatc caaacgctat tttagtcccg
    45961 caacagggcg taacccgtac gccgcgtggt gatgccaccg tactggtagt tggcgcggat
    46021 gacaaagtgg aaacccgtcc gatcgttgca agccaggcta ttggcgataa gtggctggtg
    46081 acagaaggtc tgaaagcagg cgatcgcgta gtaataagtg ggctgcagaa agtgcgtcct
    46141 ggtgtccagg taaaagcaca agaagttacc gctgataata accagcaagc cgcaagcggt
    46201 gctcagcctg aacagtccaa gtcttaactt aaacaggagc cgttaagaca tgcctaattt
    46261 ctttatcgat cgcccgattt ttgcgtgggt gatcgccatt atcatcatgt tggcaggggg
    46321 gctggcgatc ctcaaactgc cggtggcgca atatcctacg attgcaccgc cggcagtaac
    46381 gatctccgcc tcctaccccg gcgctgatgc gaaaacagtg caggacacgg tgacacaggt
    46441 tatcgaacag aatatgaacg gtatcgataa cctgatgtac atgtcctcta acagtgactc
    46501 cacgggtacc gtgcagatca ccctgacctt tgagtctggt actgatgcgg atatcgcgca
    46561 ggttcaggtg cagaacaaac tgcagctggc gatgccgttg ctgccgcaag aagttcagca
    46621 gcaaggggtg agcgttgaga aatcatccag cagcttcctg atggttgtcg gcgttatcaa
    46681 caccgatggc accatgacgc aggaggatat ctctgactac gtagcggcga atatgaaaga
    46741 tgccatcagc cgtacgtcgg gcgtgggtga tgttcagttg ttcggttcac agtacgcgat
    46801 gcgtatctgg atgaacccga atgagctgaa caaattccag ctaacgccgg ttgatgtcat
    46861 taccgccatc aaagcgcaga acgcccaggt tgcggcgggt cagctcggtg gtacgccgcc
    46921 ggtgaaaggc caacagctta acgcctctat tattgctcag acgcgtctga cctctactga
    46981 agagttcggc aaaatcctgc tgaaagtgaa tcaggatggt tcccgcgtac tgctgcgtga
    47041 tgtggcgaag attgagctgg gtggtgagaa ctacgacatc atcgcagagt ttaacggcca
    47101 accggcttcc ggtctgggga tcaagctggc gaccggtgca aacgcgctgg ataccgctgc
    47161 ggcaatccgt gctgaactgg cgaagatgga accgttcttc ccgtcgggtc tgaaaattgt
    47221 ttacccgtac gacaccacgc cgttcgtgaa aatctctatt cacgaagtgg ttaaaacgct
    47281 ggtcgaagcg atcatcctcg tgttcctggt tatgtatctg ttcctgcaga acttccgcgc
    47341 gacgttgatt ccgaccattg ccgtaccggt ggtattgctc gggacctttg ccgtccttgc
    47401 cgcctttggc ttctcgataa acacgctaac aatgttcggg atggtgctcg ccatcggctt
    47461 gttggtggat gacgctatcg ttgtggtaga aaacgtggag cgtgttatgg cggaagaagg
    47521 tttgccgcca aaagaagcta cccgtaagtc gatggggcag attcagggcg ctctggtcgg
    47581 tatcgcgatg gtactgtcgg cggtattcgt accgatggcc ttctttggcg gttctactgg
    47641 tgctatctat cgtcagttct ctattaccat tgtttcagca atggcgctgt cggtactggt
    47701 agcgttgatc ctgactccgg ctctttgtgc caccatgctg aaaccgattg ccaaaggcga
    47761 tcacggggaa ggtaaaaaag gcttcttcgg ctggtttaac cgcatgttcg agaagagcac
    47821 gcaccactac accgacagcg taggcggtat tctgcgcagt acggggcgtt acctggtgct
    47881 gtatctgatc atcgtggtcg gcatggccta tctgttcgtg cgtctgccaa gctccttctt
    47941 gccagatgag gaccagggcg tatttatgac catggttcag ctgccagcag gtgcaacgca
    48001 ggaacgtacg cagaaagtgc tcaatgaggt aacgcattac tatctgacca aagaaaagaa
    48061 caacgttgag tcggtgttcg ccgttaacgg cttcggcttt gcgggacgtg gtcagaatac
    48121 cggtattgcg ttcgtttcct tgaaggactg ggccgatcgt ccgggcgaag aaaacaaagt
    48181 tgaagcgatt accatgcgtg caacacgcgc tttctcgcaa atcaaagatg cgatggtttt
    48241 cgcctttaac ctgcccgcaa tcgtggaact gggtactgca accggctttg actttgagct
    48301 gattgaccag gctggccttg gtcacgaaaa actgactcag gcgcgtaacc agctgcttgc
    48361 agaagcagcg aagcaccctg atatgctgac cagcgtacgt ccaaacggtc tggaagatac
    48421 cccgcagttt aagattgata tcgaccagga aaaagcgcag gcgctgggtg tttctatcaa
    48481 cgacattaac accactctgg gcgctgcatg gggcggtagc tatgtgaacg actttatcga
    48541 ccgcggtcgt gtgaagaaag tttacgtcat gtcagaagcg aaataccgta tgctgccgga
    48601 tgatatcggc gactggtatg ttcgtgctgc tgatggtcag atggtgccat tctcggcgtt
    48661 ctcctcttct cgttgggagt acggttcgcc gcgtctggaa cgttacaacg gcctgccatc
    48721 catggaaatc ttaggccagg cggcaccggg taaaagtacc ggtgaagcaa tggagctgat
    48781 ggaacaactg gcgagcaaac tgcctaccgg tgttggctat gactggacgg ggatgtccta
    48841 tcaggaacgt ctctccggca accaggcacc ttcactgtac gcgatttcgt tgattgtcgt
    48901 attcctgtgt ctggcggcgc tgtacgagag ctggtcgatt ccgttctccg ttatgctggt
    48961 cgttccgctg ggggttatcg gtgcgttgct ggctgccacc ttccgtggcc tgaccaatga
    49021 cgtttacttc caggtaggac tgctcacaac cattgggttg tcggcgaaga acgcgatact
    49081 tatcgtcgaa ttcgccaacg acttgatgga taaagaaggt aaaggtctga ttgaagcgac
    49141 gcttgatgcg gtgcggatgc gtttacgtcc gatcctgatg acctcgctgg cgtttatcct
    49201 cggcgttatg ccgctggtta tcagtactgg tgctggttcc ggcgcgcaga acgcagtagg
    49261 taccggtgta atgggcggga tggtgaccgc aacggtactg gcaatcttct tcgttccggt
    49321 attctttgtg gtggttcgcc gccgctttag ccgcaagaat gaagatatcg agcacagcca
    49381 tactgtcgat catcattgat acaacatgta atcactaagg ccgcgtaagc ggcctttttt
    49441 atgcataacc tacgaacatt aaggagtaat tgaaccacca actcaggatc tcatacgaaa
    49501 accagtatta accacgtata aaattcataa aaaatactga ttgttagtta atttatatta
    49561 agtagcgcta atagatttaa taatccataa tcatttagag gctattctta attatttgcg
    49621 gtaattcttt attcattcct cggttattac gtcatattca gagcaatcct ggtattagtg
    49681 tcaccaattt catctggcga taatcctgaa atgttatgaa cagttcgagc aaactgcttt
    49741 tacctgctgc gggttagtgc tagtatgaaa aagtgagtcc tgtcccgatt ccttcctaat
    49801 tgtaattttt cgtaataatg cgatgaaaac ctgcaaagag tggcttatag ttaagctaac
    49861 aaacgagagg gcaagtccaa gtcagtaagt tttttccatc ccgaaaggtg tccgttagtt
    49921 caaccactaa gaaggggacg cgttatggat gaatactcac ccaaaagaca tgatatcgca
    49981 cagcttaagt ttctctgtga aaccctgtat catgactgtc ttgcaaacct tgaagaaagc
    50041 aatcatggct gggttaacga cccaacctcg gcgatcaacc tccagttgaa tgaactgatt
    50101 gagcatattg cgaccttcgc acttaattac aaaattaagt ataatgaaga caataagctc
    50161 attgagcaga tcgacgaata tctggatgac acctttatgt tgttcagcag ttatggtatt
    50221 aatatgcagg ctcttcagaa atggcggaag ttaggtaatc gactattccg ttgttttgtc
    50281 aatgcgacga aagagaatcc tgcgagttta tcttgttaga attattacaa ccatgggtag
    50341 aagtatgtcc gaaaaacctt taacaaaaac cgattattta atgcgtttac gtcgttgcca
    50401 gacaattgac acgctggagc gtgttatcga gaaaaataaa tacgaattat cagataatga
    50461 actggcggta ttttactcag ccgcagatca ccgcctcgcc gaattgacca tgaataaact
    50521 gtacgacaag atcccttcct cagtatggaa atttattcgc taataaataa ttcgctttcg
    50581 gagctataac cggctgttta ttaagaattt tatacttttt cgccatgaag acatacccta
    50641 tgtgatcttt atcacacaga tgtaatgaga acgttctctt cactgacttt tcgtcttact
    50701 gtgttgccgc attttcagca accggaggtc agtaatgagc acagaaaaag aaaagatgat
    50761 tgctggtgag ttgtatcgct cggcagatga gacgttatct cgcgatcgcc tgcgcgctcg
    50821 tcagcttatt caccgataca atcattccct ggcggaagag cacacattac gccagcaaat
    50881 tctcgctgat ttattcggtc aggtgacaga ggcttatatt gagccaacgt ttcgctgtga
    50941 ttatggctat aacatttttc tcggcaataa ttttttcgcc aacttcgatt gcgtgatgct
    51001 tgatgtctgc cctattcgca tcggtgataa ctgtatgttg gcactaggcg ttcatatcta
    51061 cacggcaaca catcccatcg accctgtagc acgtaatagc ggtgctgaac tggggaaacc
    51121 cgtcaccatc ggtaataacg tctggattgg cggacgcgcg gtcattaacc ctggtgtgac
    51181 cattggtgat aacgtcgtgg tagcttcagg tgcagttgtc acaaaaggtg tcccggacaa
    51241 cgttgtcgtg ggcggtaatc cagccagaat aattaaaaaa ttgtaatcgg tttttcgcaa
    51301 ctgttatgca aaattgtggt agatctgtta cttcccctct actattccca cgttaaaata
    51361 gggtgttccc tggaaagttg cagataccac gaaggcaaac gatgaccgaa atacaacgcc
    51421 tgctgaccga aacgattgag tctctgaata cccgcgaaaa acgcgacaac aaaccccgct
    51481 ttagtatcag ttttatccgt aaacatccgg ggctgtttat cggtatgtac gttgcttttt
    51541 ttgccaccct ggcggtgatg ttgcagtccg aaacgctgtc aggctctgtc tggctactgg
    51601 ttgtattatt tatcctgctt aatggtttct tctttttcga tgtctaccca cgctaccgct
    51661 atgaagatat cgacgtgctg gatttccgcg tttgctataa cggcgaatgg tacaacacgc
    51721 gctttgtacc tgccgcgctg gttgaagcca tcttgaactc tccgcgtgtc gcggatgttc
    51781 ataaggaaca actgcaaaaa atgatcgtcc gtaaaggtga actgtctttt tacgatattt
    51841 ttaccctcgc tcgcgccgaa tcaacatctt aagttagggt tacataccag gcgtaaagct
    51901 ctgcgcctgg tcaaatgaca atgatcgttt ccacccatca cttcatgaaa taccagctct
    51961 acctccttat ctcctgccag cctttttcca caatcagata tactttccct acactgtgtt
    52021 aataaggata tgctggtgag aacacgacat ctggtcggcc ttatttcggg agtactgatt
    52081 ctttcagtat tgctgcctgt cggcttaagc atctggctgg cccatcagca ggtagaaaca
    52141 tcgtttattg aagagctgga tacctattcc tcccgcgtcg ctattcgagc caataaggtg
    52201 gcgacacaag ggaaagatgc gctgcaggag ctggaaagat ggcaaggcgc tgcctgtagc
    52261 gaagcccatc tcatggaaat gcgtcgggta tcttacagtt atcgctatat tcaggaagtg
    52321 gtttatatcg ataacaacgt tcccctgtgt tcgtctctgg agcatgaaag tccgcccgat
    52381 acctttcccg agccaggtaa aatttcgaaa gatggttatc gtgtctggtt aacatcgcat
    52441 aacgatttag gcattatccg ttacatggtc gccatgggaa cggcacatta tgtcgtcatg
    52501 atcgaccccg cttcctttat tgatgtcatt ccctatagct catgacaaat tgatgccgcc
    52561 attattggca atgcccataa cgttgtcata accagcagcg atgaaattgc tcagggaatt
    52621 attaccaggc tacaaaaaac acccggtgag catatcgaaa ataatgggat catttacgat
    52681 atcctgccct tcccggagat gaatatttcg atcatcacct gggcttcaac gaaaatgttg
    52741 cagaaaggct ggcatcggca agtctttatt tggttaccgc tcgggttggt gattggcctg
    52801 ctggcagcga tgtttgtgct gcgtattttg cgccgtatcc agtcaccgca tcatcggctg
    52861 caggatgcta tcgaaaatcg tgatatttgc gtgcactatc agccgattgt ctccttagcc
    52921 aatggcaaaa ttgtcggtgc tgaggcactg gcgcgctggc cgcagacaga cggtagttgg
    52981 ttgtcaccag atagttttat tccgctggca cagcaaacgg gcctttctga gccattgacg
    53041 ctactgatta taagaagcgt ctttgaagat atgggcgact gcttgcgtca gcatccacag
    53101 cagcatattt cgatcaatct tgaatccacc gtgctcacct cggaaaaaat cccgcaattg
    53161 ctgcgtgaaa tgatcaatca ctatcaggtt aatcccagac agatcgcgct tgaactcact
    53221 gaacgcgagt ttgccgatcc gaaaaccagc gccccgataa tttctcgcta ccgggaggcg
    53281 ggccatgaaa tttatcttga tgattttggt acggggtatt caagtttaag ttatttacag
    53341 gatctggatg tcgacattct gaagatcgat aaatctttcg ttgatgcgct ggaatataaa
    53401 aatgtcacgc cgcatatcat cgaaatggca aaaacactga aactgaaaat ggtagcggag
    53461 ggaatcgaaa ccagtaaaca agaagagtgg ctacgccagc atggcgtgca ctacggtcag
    53521 ggctggctct acagcaaggc attaccgaaa gaagatttct tacgctgggc cgagcaacat
    53581 ttgtgaattt ttgggctgac ggcacgccgc acagcccgtc acgctaccta ttcgtcgatg
    53641 attgactcga atccgccata gatcattcgc ttgccgtcaa acggcatgga ctcgccgaac
    53701 tctttcatcc gtgggtccga catcatcttt tgattagctg cgtcgcggac ctcttttgaa
    53761 gggtattcaa tccagctaaa gacaacctct tcattctctt ccgctttcac cgccatacga
    53821 aaatcggtca ctttgccatc cggtacatcg ctggcccagc attcgacaat acgaagcgcg
    53881 ccaaactctt taaacaatgg cgcggcctta gcggccattt cccgataggc atccttttta
    53941 tcggcaggaa cggcaaccac aaaaccatca acatacttca tcagacatac ctccgaagcg
    54001 tactgcgcag ccagacaatt cttgcgacgt cagttgtggt taaagtttag ccatgctggg
    54061 attttttgac gaaacggtgg gaggcagaaa tgggtggggg ccctgccagc tacatcccgg
    54121 cacacgcgtc atctgcctta gctgcttcct tccggacctg acctggtaaa cagagtagcg
    54181 ttgcgggaga accaacagag cccccattga gagcgttgaa aaccaacgcg caggcgcatt
    54241 atcactgctg ccccggctaa ttgcaaccca ttgcgtgcca gatgctggtt tcttgcgcaa
    54301 tgcgacttca ctcgggcgtt tttccagatt atgctgaaaa attacctcag gaagaaaaga
    54361 tggaaaaaga agattcattt ccccaacgcg tctggcaaat cgtcgccgct attcccgaag
    54421 gctatgtcac cacttacggt gatgtggcga aactggcggg atcgccccgc gccgcgcgcc
    54481 aggtgggcgg tgtgttaaag cgtctccctg aaggcagcac cttaccctgg caccgggtgg
    54541 ttaatcgcca tggcacaatt tcgctaaccg ggccggattt acagcgtcag cgacaggcat
    54601 tactggcaga aggtgtgatg gtatcgggaa gcgggcaaat cgacttgcag cgttatcgct
    54661 ggaactacta accctctccg ggcggagagg gttaacgtcg cttaatattg cgtaggagcg
    54721 ggaactgccg aagacggatt cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg
    54781 ccaccgctgg cctgaaccgg cacggcggtt tgctgtaccg gcaccaatgt caggtcggct
    54841 ttagttccgc cctggttgat caccggctga acggtatcgg tgataaatac cagtttgtca
    54901 ttcacgttaa tcgccgcact caacagaata cgcgcgttcg gctgaacatc tgccgggtta
    54961 aatggcagaa caaagctgaa tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc
    55021 agcacttttg acggtgcatc ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca
    55081 tcaggcggca gtgcgacttt ctgacggatc cagacggtac cggagacatt cggttgctgg
    55141 atagcaggtt gttgtgttgc tgaaatagac gtatttgcag ccggggctgg cgtctgaata
    55201 tccgcgcttt tatctgcgca agccgccaac gcaatcgcaa ccgctaaacc actggccatg
    55261 tgcacgagtt tcattcattt ctccttatta tcaatgcacc agcgggctaa ctttcctcgc
    55321 cggaagagtg gttaacaaaa tagtaacgtc aacaagtgtg gcacacatca cgcatttctg
    55381 cctgtaatta gcccgtaatt cagactattg cacccatcgg accagtggca aatttgcgtt
    55441 atactcaact cactttggct tgctgcggca gctttgttac tggagagtta tatgagtcag
    55501 gcgctaaaaa atttactgac attgttaaat ctggaaaaaa ttgaggaagg actctttcgc
    55561 ggccagagtg aagatttagg tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc
    55621 ttgtatgctg caaaagagac cgtcccggaa gagcggctgg tacattcgtt tcacagctac
    55681 tttcttcgcc caggcgatag taagaaaccg attatttatg atgtcgaaac gctgcgtgac
    55741 ggtaacagct tcagcgcccg ccgggttgct gctattcaaa acggcaaacc gattttttat
    55801 atgacggcct ctttccaggc accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc
    55861 gcgccagcgc ctgatggcct gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg
    55921 ctgccgccag tgcttaaaga taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg
    55981 gagtttcata acccactgaa aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc
    56041 gcaaatggta gcgtgccgga tgacctgcgc gttcatcagt atctgctcgg ttacgcttct
    56101 gatcttaact tcctgccggt agctctacag ccgcactgca tcggttttct cgaaccgggg
    56161 attcagattg ccaccattga ccattccatg tggttccatc gcccgtttaa tttgaatgaa
    56221 tggctgctgt atagcgtgga gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt
    56281 gagttttata cccaagacgg cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt
    56341 aatcacaatt aaaaaaatag ccggaggtga aaaccgtccg gctatttttt gcagtgcttg
    56401 ttacgcgtta taggcattct cgccgtggct gttgacgtcc agcccttctc gctcttgttc
    56461 ttccggcaca cgtaggccaa ccgtcagatc cgccagtttg tagccaataa atgccaccac
    56521 accggaccag acgatcgtaa tggcgatgct ttccaactgt accagcaact gatggcccat
    56581 cgtcacacct tcagcgaagc ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat
    56641 gatacagccg acaatgccac aaacgccgtg cacaccgaag acatcgcagg gatcatccac
    56701 ccgcagcaag cgtttgagca tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc
    56761 gataatcaac gcgccgccaa ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc
    56821 ggcaatcgcg ccagaacacg ctcccagcag agaaggctta ccacgcagcg cccattcacc
    56881 gaagatccag ccaagaattg ccgcctccgt tgcgaccaca gtattcacaa atgccagtgc
    56941 cgcgatttca ttcgccgtgc ccgctgaccc ggcgttaaag ccaaaccaac cgatatagag
    57001 aatggcagtc ccggtgaaga ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa
    57061 gcccacgcgt ttgcctatca gatacgcgcc caccagaccg gcgattgctg cgttaatgtg
    57121 caccaccgtg ccacccgcga aatccagcgc accgtgagaa gccagcaaac caccgcccca
    57181 caccatatgc gcaatcggaa tgtaagagag cgtcagccat accaccacga aaatcaacac
    57241 agctgagaag cggattcgtt ccgccagcgc cccaactatc aagcctacgg taatgcaggc
    57301 aaacgatccc tgaaacgcta cgtggatata ctgataaatg ctgcccatca ccgccgtcag
    57361 ttcgatgttt tttagcatca accagttaat gttgccgaag aagttgttgc cctcaccaaa
    57421 cgccagcgaa taaccgtaaa ccacccagag aatacagacc agtgcaaacg tcaccgtcac
    57481 ctgcgtcagc atcgacagca cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat
    57541 ccccggaata gtcataaaca gcaccagcgc agtacaaatc atcataaacg cattgtcggc
    57601 tttatcggcc accgcaggtg cagccattac cagtcccggc agcatcgcca gtgaagcaag
    57661 cccagttttt atcgtcgcta tcttcatttt tcgttcctgt tgctgtgtgc cagagattac
    57721 agcgccgctt cgtcggcttc gccggtacga atacgaatga cgcgttgcaa ttcagcgacg
    57781 aagattttgc cgtcgccaat ttttccggtg taagccgcct tactgacgat atcgatcact
    57841 tcatcgagtt ggtcatcagc aatcgccaca tcaattttta cttttggcag gaagttgacg
    57901 ctgtattccg ccccccggta cagctcggca tgccctttct gacgcccgaa acctttcact
    57961 tcggtgacgg tcaggccctg aataccaatg gaagataacg cttcacgaac gtcttccagc
    58021 ttgaatggtt tgattatcac ggtcaccagc ttcatagatc ccctccggtc agaattcggt
    58081 aatggttctg ctacacgaag aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa
    58141 agagagcagg aagttaagaa gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt
    58201 tacatttttg caccaacgat gaaatattgc gctattcagg cgctcaatga ttcctcttca
    58261 cgcacgctgg ctgccagctc ttcgcccgca agttgcagtt gatacatctg ccagtagcgt
    58321 ccctgggccg ccagcagttg ctggtgagtg ccctgctcca cggcttgccc acgatgaagc
    58381 accagaatgg tgtcggcatc aacaatagtt gataagcggt gagcaatcac caccagcgtg
    58441 gtatgttcac gcaccgccgc cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg
    58501 ctggcggttg cctcatcaag gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt
    58561 gccagcagtt gcttttgccc gactgagaga ttatttccct gctcgcccag cggcgtataa
    58621 ataccatcac tcatgctgcg cgccagctcc gccagttgca cggtttccag cgcctgccag
    58681 acgcgttctt cggagatatc ccgccccagc gtcacgttgg cgaggaaggt atccgccagc
    58741 accaccggat cttgctgcac cattgccacg ccctggcgca gcgcgctatg acttagcgaa
    58801 cttaatggac gaccatcaag gtgaatctca ccttccgtta gcgggtaata gcccatcaat
    58861 aaactggcga gggtactttt accactgccg gtatgcccga ccagcgccac aaagttacgc
    58921 gaaggcacag agagattaat gttctttagc accagattgt catcgcgata agcaaatgac
    58981 acgttatcga cttcgatggt gccactctgt aacgggtaat catcattgcc atattgctgg
    59041 cgcggtccgt ccatcagttc aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc
    59101 gcctgttgcg tggtcagttc gattaagggt tcgttaagtc gcccaagata gctgataaac
    59161 gcatacaaca cgcccacttc aatggtgccg ctggcggaga agccaaacag catcaataag
    59221 ccacaaagaa tgagcgatga aaacagactc agcagcggac gcagcagaaa accgtcgagg
    59281 cgcagggttt gcatcctcgc catatagtgt gaacgactgg cctcccccat acgttcgcca
    59341 aatcgcgcct gctgacgaaa ctgctggata acgctcatgc cattgatgat ttcgttaaag
    59401 ccgtcgttga tatccgccag ataggcgcgc acacggcgga caatcggcgt gctgtaacgc
    59461 tggtatatca ccattaccac cagcaccacc gggaaaatca ttatcgccac cagcgccatt
    59521 cgccagtcga ggctgaacat cgccaccagc atcgcgccca ccagtgcggc actgcgcagg
    59581 acagttgcca ctacggtaac gtagagatcg cggatcactt cagtgtcatt agtgacgcgg
    59641 gaaatcacct gcccgacggg ttgggtatca aactcgctta atggctggcg taacgcagca
    59701 tccatcacgt cggtacgcaa ctgttgcact acgcctactg ccgcccgatt aaacagcagc
    59761 gactgcgcgt aatgtagccc ggcggcaaac agttgtagcc caacatacgc cgcagccagc
    59821 cctgcaacca ctttcaacgg caggttattt ttcgctacca tattgtcgat aaaatagctg
    59881 ataagcagcg gcccactgac ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc
    59941 cccagcggtt tacgccacgg cgaaccatac gctaacaggc gcttgagagt cggccacagt
    60001 tggctaaaac tacgcatcga cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc
    60061 ctccagttgt tgatagcgat acatatcgcg ataccagccg ctttgttgcg ccagctcatc
    60121 atgattgcca cgctgggcga tatgtccgtg ctgcatcaca ataatttcac tggcttccgt
    60181 cagtgccgaa aggcgatggg cactgatgat taccgttctt ccctgtcccc actgacgcag
    60241 gttatgcagg atctggtgct ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat
    60301 gaggatttcc gcgttgacta ataacgctcg agcaatggag atacgctgtt tttgcccacc
    60361 ggaaagcatc acgccgcgct cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat
    60421 atcgtcatgt acgctggcta accgcgcgac atgctcaatc tcttgctggg tggcattcgg
    60481 gcaacccagc gcgatgttat tcgccacggt gtcagaaaaa aggaatggcg tctggctgac
    60541 caccgccagg cggctacgcc agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa
    60601 gcgaatatcc ccctcgctga cgtcgaaatg acgctgaatg agcgacaaca gggtactttt
    60661 gccggaacca gtcggcccgc aaatacccag catctgaccg ggtttcagga cgaaattgac
    60721 gttttccagc gcaggatggt cagtctgcgg atacgtgaac tggtgaatat ttacatccag
    60781 ttcgccacgc ccttccggca cgggttcgct accatcgttc accaccggcg cttccgccag
    60841 catcgcgcga atacggctgt atgcagcact accacgttcc acaatgttaa acatccatgc
    60901 cagcgccagc attggccaaa tcatcagacc taaatacatc ataaaactgg tgagctggcc
    60961 cagcgttaaa ctgccctgca ccaccatcca actaccgccg ccaatcgcca gcaagttcgc
    61021 cataccaatc gcgatataga tggtcgggtc gaaacgagca tcaatacgcg ccacccgcat
    61081 gttttttttg ccggtatctt tggcatccac ggcaaataac gccgactggc gatcttccag
    61141 accaaaggct ttgatcatgc ggatactggt gaggctttcc tgggtgcggt cattaagact
    61201 ggaaaacgcc gcctgtgcca gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat
    61261 catgatcgcc atcactggca tcggtaacag ggcaaataag gtcaactgcc agctaatttg
    61321 cgtagacatc ataatcaaca cagcgcagcc cattaccagt gaatccacca gcgtcagcac
    61381 cccttctccg gcggcaaaca cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc
    61441 acccgtgcga tgacgcaggt aaaactccgg gtgctgccgg cttagctgac ggtaataatc
    61501 ttcacgcagt tcaacagcca gttggtaaga cgcgccaaac agcaatacgc gccagacata
    61561 acgcaggaga taaaccacaa cggcaatcag caccatggtg gcgatccaca tcaggatcag
    61621 cccggtagta aagtgttgtt ctgtcacgcc atcgacaaca ataccaacca cttttggcgg
    61681 aaccagttgc agcatcgcga taatgacaag caaggcgaca gccccaagat agcgacgcca
    61741 ttcccgacgg aaataccagc ttaattgagc aaataatcgc acgcgttgtg tcctgacctg
    61801 attctggata tttattcgat gggtaaagaa gtggtgtatt taatctgttc catcgcgaag
    61861 ctggaagtga cgtccgacag ccccggcacg ctgtttacca gacgcttata aaactcgtcg
    61921 tagcgtttca tgtcggcaac ctggacgcgc atcagataat cgtattcacc agccatgcgc
    61981 cagaacccca gcacttctgg catttcggta accaccgtga caaagcggca ataccattcg
    62041 ctgctgtgat gttgcgtttt tatcagcaca aaggcggtca ggccgaggcc tattttttcc
    62101 ggatccagca gggcgacttt gccgataagg ataccgtcgt cctccagccg tttcaggcgc
    62161 ttccagcagg gggtggttgt cagattaacg gcttcagcca gtgcctgcaa agagagggtg
    62221 caatcctgct gcagtaaggc cagcagctta cggtcaattt tatctaacat agcccttcca
    62281 cggagaattt ttttctcttt taattctatt ttaaaggtga aatcgcgaca atttattctg
    62341 tgcggtttaa acaccacggc acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt
    62401 gcccggtgct ggaagggatc tcgttattcg ggggaataag gtagatgtgg atagtccagc
    62461 cagtgcgtca aatagtgaga gacagcctga tttcggcaat gtccaatcac cggtaaatgc
    62521 gggagctccg cgcgcagttg cggcatcgca ttgcccataa taaacccgct accgacgctg
    62581 cctaacattt cgcgatcgtt catcgcatca ccaaaggcca tgcaatcgcg caacgataaa
    62641 cctaaatgtt gggtcagcac cgtcaatgca gcgcctttat tgcagcccac cggcagcact
    62701 tcgaggcaat acgtggcgga aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc
    62761 tggatctgca agcgtgtaag atcgtcgtga tcgccacaga agcagatctt ggtgacgctg
    62821 ccgagtggca tttttttgac atcgattatc tgataacgaa aaccgctata gacaaatgcc
    62881 tgcaacaacg cagggatctc tttcccggta aaccaaccgt cgtcattgaa gatatgcatg
    62941 ctggctcggg tatcccattg ctgatacagc accagctccg cgacatccgc aggtaaatca
    63001 tcacgatgta aaagttcacc ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa
    63061 tatgcatcca gcgatagcgc cccgagaata tgctgcatct ccagcgcatg acgccccgtg
    63121 gcaaaagtga gggtaatgtc gcgttcacgc agtcgcgcca aagtagagag ggttttctca
    63181 cctaaatgat ggtcaggcat caataaagtg ccatccatat caaatgctgc cagacgagcc
    63241 atttctttct ccactctgtg acacggttaa tggtgattga gtatcacctg atatatacgg
    63301 aagtaatagt gaatagttaa ataatattgt tccgggttta tatgcgattg ctcaaccgtc
    63361 ttaaccagta tcaacgtctg tggcaacctt cagccggaaa gccgcaaacc gtcaccgtca
    63421 gcgaactggc cgaacgctgt ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg
    63481 cacaggaggc gggatggctg gagtggcagg cgcagtcagg acgcggaaag cgcggacaat
    63541 tacgctttct ggtcacgccg gaatcgctac gcaatgcgat gatggaacag gcactggaaa
    63601 ccggaaagca gcaagatgtg ctggagctgg cgcaactggc cccaagtgag ctgcgcactc
    63661 tgttacagcc gtttatgggc ggacaatggc aaaacgatac acccacgttg cgtattccct
    63721 actatcgccc gctcgaaccg ctacaaccag gctttttgcc cggccgtgcc gagcagcatc
    63781 tcgccgggca gatattttcc ggcctgaccc gcttcgataa taatactcag cgcccgattg
    63841 gcgatttagc gcatcactgg gaaacctcta ctgacgggtt acgctgggac ttttatcttc
    63901 gttcaaccct acactggcat aacggcgatg cagtaaaagc ctcacactta caccagcgat
    63961 tattgatgct gttacaactg ccagcactgg atcaattatt tattagcgtg aagcgtattg
    64021 aagtcaccca tccgcagtgt ctgaccttct ttttacatcg ccccgattac tggcttgcgc
    64081 accggctggc gagctattgc agccatctgg cgcatccgca attcccactg atcggcactg
    64141 gtccttttcg cttaacacaa ttcacagcgg agctggtgcg cctggagagc catgattatt
    64201 accatttgcg tcatccgctg cttaaagcgg ttgagtactg gataactccg ccgcttttcg
    64261 aaaaagattt gggaaccagt tgtcggcatc ccgtgcaaat caccatcggc aaaccggagg
    64321 agctgcaacg ggtcagccag gtcagtagtg gcatcagttt aggtttttgc tatttaacgt
    64381 tgcgcaaaag tccccgactc tccctctggc aggcgcgaaa agtgatctcc attattcatc
    64441 aatccggttt attacaaacg ttagaagtcg gagaaaacct gatcaccgcc agtcatgcat
    64501 tactgccagg ctggactatt ccgcactggc aagtaccgga tgaagtcaaa ctaccgaaaa
    64561 ccttgacgct ggtttatcac ctaccgatag aacttcatac catggcagaa cgcctacagg
    64621 cgacactggc agcggaaggc tgtgaactca caattatttt tcataacgca aaaaactggg
    64681 acgacacgac cctactggca cacgcagacc tcatgatggg cgacagatta attggcgaag
    64741 caccggaata tactctggag caatggctac gttgcgatcc actatggcca catgttttcg
    64801 acgctccagc atatgcacat ctgcaatcga cgctggacgc ggtgcaagta atgcctgatg
    64861 aggaaaaccg atttaatgcc ctgaaagcgg tttttagcca gttaatggca gacgcgacgc
    64921 tgacgccgct gttcaactat cactatcgca ttagtgcccc tcccggcgtg aacggtgtgc
    64981 gactgacacc gcgcggctgg tttgaattta ccgaagcctg gcttcccgcg ccgtcgcaat
    65041 gaatagctgg tccgggttaa tcgcaggcgt taccataacg cttttattgt tcaagcagga
    65101 ttatctatga aacgtgctgt cgttgtgttc agtggaggcc aggattccac cacctgtctg
    65161 gtgcaggcat tacaacaata tgatgaagtc cattgcgtga cgttcgatta cggtcagcga
    65221 catcgcgcag aaatcgacgt ggcacgcgaa ctggcgctga aactgggagc acgcgcgcat
    65281 aaggttcttg atgtaacgct gctcaacgag ctggcggtca gcagcctgac acgtgacagc
    65341 attccggtgc ctgattatga acctgaagcc gatggcatcc cgaatacgtt tgtcccaggg
    65401 cgtaatattt tgttcctgac gctggcggca atatatgcgt atcaggtaaa agcagaagcc
    65461 gtaattaccg gcgtctgtga aacggatttc tccggctacc cggattgccg cgatgagttt
    65521 gtgaaagcac taaaccatgc cgtcagtttg ggcatggcga aagatattcg ttttgaaacg
    65581 ccgctgatgt ggattgataa agcggaaacc tgggcgctgg cagattatta cggcaaactg
    65641 gatttagtcc gtaacgaaac gttgacctgc tataacggca ttaaaggcga cggttgcggt
    65701 cattgtgcgg catgtaattt acgcgccaac ggtttgaatc attatctggc cgataaaccg
    65761 acggtgatgg cagcgatgaa gcagaaaacc gggttgaggt aattattccg ggtgtcgccg
    65821 gatgcggctt gagcatccgg caccacaaaa cgtttactta accatctgct ccagcttttc
    65881 gcgcaattcc ccttccagag ctaatgcttt ctgcgtttta agatcaatac aaacaaacgt
    65941 aataagcgca tccgctacca cctgcccttc cggctccagt gtaatgacct ggcttaagat
    66001 gccgctttta ccgtttaatt gctgcaactg actggtaata gttaacaggt cgcttaatac
    66061 cgctgggcga cgatagttaa tattgatatt gaccacgacg aaggcgatgt tatgggccgt
    66121 catccactga aaactgtcgc tattttccaa cccatcccag cgggcttctt cgagaaattc
    66181 aaggtagcgg gcgttgttga cgtgctggta aacgtcgaga tgatatccac gaactttgat
    66241 ttgtgtttgc atagcgcaat aaccttacgt tgttgttata agtacagagg tcataactgg
    66301 tatgacctct tcagtctggc aaaaaattgc cactatgcaa attaattaca gggttaatac
    66361 cgccagatta cgttccacca gcgaattgcc catccccggc acctgcttta gatcctccac
    66421 cgttttaaac ggaccgtact cttcgcgata actgacaatc gcctgcgctt tcttcaggcc
    66481 aacgccattc atcgcgcggg ctagctcttc cgcgctggca ttattaatgc tgacccgggt
    66541 gccttcttcg tcactggctt tcgccggtac tgctgcttta ctttgtgctg caggagcttc
    66601 cgctttggtt tctaccgccg tcggtttcgc cacagaagct gccgccagcg cgctatgaga
    66661 cattccggca caggccaggg acagggtaat gagcagtgct ttaattccgt gtttcatact
    66721 gttttctcct tgtttgttaa cagtggaatc accttaacgg gagtgaaaac agtaaacaaa
    66781 tggcaatgtt cagaaatgga aaaggccgcg aaagcgacct tttcttgtta cagctgcaac
    66841 gcattttttt gcgaggcggc ttcaggatta ttgctgttcc agcgcatcgc caattttgat
    66901 tttcgcctct ttacgcaggt tactcatcag agcttcaaag acgatttgtg cgttgttctg
    66961 ggtgataccc tgcaccatcg cttttttctg atcttccggc attgaacctt gtttcacttc
    67021 atccagcgcc agcagaacca cattaccttg catatcggtc gccataccgt agctcggttt
    67081 gtctttcgct ggcagtggca gtgcaaacgc cgcctggcta atcgggtcac gaccggaacg
    67141 gcttaaggtt ttcggctcgc caaatttcag accggcagcc tgcatagctt ccgcaccttt
    67201 gccggctttc aaatcaacca gcagtttctc agcatccact ttcgcctgtt gttcagcttt
    67261 gttgtgctga accagtgcct taacttgttc ctgaacatct gccaacggtt tcaccgcttc
    67321 cggtttgtgc tcgctgatgc gcagcacgaa tgcgcggtcg ccgtctacgg tgatgatgtc
    67381 agagttgatg cccggcgcgc cgttttcacc taccagaccg ccgttaaaga tagcgtcggc
    67441 aaccggcttg aagttcaact cttccggcag gttatctttg ctgaaccaac ccgtctgagt
    67501 ggctttaacg ccggcagctt gctctgcacc ggccagagac tcggtgtcgt tgcttgccgc
    67561 atcgctcact ttctgctgca gcgcatagta cgcatcgagg gctttttcgt gtttcacttt
    67621 cgccgcaatg tcgtcacgta cttcgtctaa cgatttcact ttcgctggct gaatgtcgtc
    67681 cagacgtaca atcaggaaac cgaccgaaga tttgatgaca ccagacagtt ggcctttttc
    67741 tttcagacca gcatttttca gttcgtcggg gatagtggca tcttctaacc aacccatatc
    67801 gccgccgtta cgggcagaga taatatcggc agatttttct ttggctaatg cagcaaaatc
    67861 accgccttta ttcagctcat caagtaccgc tttcgcttca tcttcagttt tggtctggat
    67921 gatgctgtag cgggtacgct gcggctgggt gaattgatcc tgatgctgat cgtagtagct
    67981 ctggatatcc gcatcgctaa ccggttgctg catcgttgcg gcaaccagct tgatgtaact
    68041 cacgcggaat tgttccggcg tcatgaaatt gtttttgttt tgttcgtagt agctggcaat
    68101 ctcctgttcg gtcacaggct gcttcgccgc cagcgcgtta acatcgatag tcgcctcacg
    68161 caccacgcgt tgttgcgcga ccagtgccgc cagctcgtcg gtttcacctt tcagcataaa
    68221 atcggtaccg gcaacgccgt taatcagctg ttgggtagtg agctggttac gcagcgcctg
    68281 ggcgtactga tcggcggtca tccccatctg gttgaggata ccgttatagc ggctgttatc
    68341 aaatttgccg tcaacctgga aggctggggt cgcgaaaatc gcctgtttaa cctgctcatc
    68401 actgataccc agtttcagct cacgagcgta ctgatccaac agcgcctcgt cgatcagacg
    68461 attcagcacc tgttgacgca gggttttcat atagccttcg ttcgctgcca gctcggagta
    68521 ttgatcgccc agctgttgct gcatgcgatt acgctcgctg ttaaaggcgt tctcgaactg
    68581 cccacggctg atttcctggt cattcacttt tgcggcgtag ttattgcctc cgccaatcag
    68641 gtaaccactc acgccggtca atatgaacga cacgataatg ataccgaaaa taatcttgag
    68701 cacgagactg tttgcagccg tgcgtaagct gtccatcatg gtgtaacaac actccgctgt
    68761 agatgactgt gtacctcacg caacgtatcg atgcgcgcaa aggcctattg tgacaagaaa
    68821 cgggggcaat tgtcagccca caagacacag aaagtcaccg aatacaaata aaaaaggcac
    68881 atcagtagat gcgcccttga acttcgtcac atccccactg gggacaacgc ttagtttacc
    68941 gcgtctttca gtgctttacc tgcacggaag ctcggtactt tagcggcagc gatggtgatc
    69001 tctttaccgg tctgcgggtt gcggccagta cgggcagcac gctctttaac ggcaaaagta
    69061 ccaaaaccta ccagtgctac atcatcccct tctttcagag attcagttac ggaagcaata
    69121 atagcatcta acgcacggcc agccgcagct ttagagatat cagcccctgc agcaatcttg
    69181 tcgatcaatt gagatttatt cactcttctc ttcctcttta taatttatat cgcacttgaa
    69241 tccttcaagg tacgaacgcg cagcagttat atcaggccag ccatcccctt acaacacccc
    69301 ttaataagga caagcccgat ccgctatcta acttagcgag acaaaaaaag gctggcaagc
    69361 ccgaattagc ctgccagccc tgtttttatt agtgcatttt gcgcgaggtc actattttgc
    69421 agtcacaacc tgcatgccag acggttcatt ttgcagcgcc agagtcagaa cttcctcaat
    69481 gcgcttcaca ggatgaatgt ccagatcggc aattacgttg tcaggaatct cttccagatc
    69541 gcgtttattt tcgaacggaa ttagcactgt tttaatcccg ccgcgatgcg ctgccaggag
    69601 tttttctttc aaaccaccga tcggcagtac ctgaccacgc agagtgatct caccggtcat
    69661 tgccacatcg gcacgaaccg ggttaccggt caggcaagaa accagcgcgg tgcacatagc
    69721 aataccggca ctcggaccat ctttcggcgt cgcaccttcc ggtacgtgga cgtggatgtc
    69781 acgtttttcg taaaaatcag ggttgatccc cagtttttcc gcacgcgcac gaaccaccgt
    69841 taacgccgcc tgaatagact cctgcatcac ttcgccgagc gaaccggtat aggtcagttt
    69901 gcctttaccc ggaacacatg cggtttcaat ggtcagcaag tcaccgccca cttccgtcca
    69961 cgccagaccg gttacctgac cgacacggtt ttcgttatca gcgcgaccat agtcgaaacg
    70021 ctgaacaccg aggtagtcat gcaggttatc gccgttaatt tcgatatgtt ttaatgactt
    70081 atcgagcagt aactgcttaa ccgctttgcg gcacagtttg gagatttcac gctccagacc
    70141 acgcacgccc gcctcacggg tgtagtaacg aataatgccg ataatggcgc tatcgtcgac
    70201 ggtcagctca ccttttttca gtgcattacg ttcaatctgc ttcggcagca ggtgacgttt
    70261 ggcgatgttc agtttttcat cttcggtata accggagagg cgaatcactt ccatacgatc
    70321 cagcagcggt gccggaatgt tcatggagtt cgacgtcgcg acaaacatca cgtcgctgag
    70381 atcgtaatcc acttccaggt agtggtcgct gaacgctacg ttctgctctg gatccagcac
    70441 ttcaagcagt gcagaggccg gatcgcctcg catgtcagaa gacattttgt cgatctcatc
    70501 gagcaggaac agcgggtttt tcacgcccac tttcgccatt ttctggatca gtttacccgg
    70561 catagaaccg atgtaagtac ggcggtgacc acggatttcc gcttcatcac gcacgccgcc
    70621 cagcgccata cggacatatt tacgcccggt ggctttggca atggactgac caagagaggt
    70681 tttacctacc cccggcggcc ctaccaggca gaggatcggt cccttgattt tgttgacacg
    70741 gctttgaacc gcaaggtact caaggatgcg atctttcacg cgctccagac cataatggtc
    70801 ggtatcaagg atttcctgcg cctgacgcag gtcttttttg accttgctgc gcgcattcca
    70861 cggtacctgt accatccagt cgatataacc acgcactacg gtcgcttctg ccgacatcgg
    70921 agacatcatt ttcagcttct gcaactctgc ttccgctttc tcttttgcct ctttcggcat
    70981 cttcgccgcg tcgattttgc gcttcagggc ttcgttttcg tccggcgcgt cgtccatttc
    71041 accgagttct ttctgaatag ctttcatttg ctcgttcaga tagtactcac gctgggattt
    71101 ctccatctgc tttttaacgc ggttgcgaat gcgtttctca acctgcagca gatcgatttc
    71161 cgattccatc attgccatca gatattccag acgttcgtta acgtcggaca tctccagaac
    71221 ggactgtttg tcagccagtt tcagcggcat atgtgcagca atggtatccg ccagacgcgc
    71281 cggatcgtcg atgctattca gcgacgtcag cacttctggt gggatttttt tgttcagctt
    71341 gatgtagcct tcgaactggc tgattgcagt acgcaccagc acttcctgtt cccgctcatc
    71401 aatggtcggc gactccagat actccgcctt cgcagaaaag tgttcgccat tgtcagagag
    71461 cgcagaaata cgcgcgcgct gtaacccctc gaccagcact ttgacggtgc cgtcaggcag
    71521 tttcagcatc tgcaatatag aggccacggt cccgacggtg aaaagatcgt ttacacccgg
    71581 ctcatccgtt gaagcttctt tctgcgcgac cagcataatt tttttatcat ggtccatcgc
    71641 cgcttccaga caacggatag atttttcccg cccgacaaat aaggggatga ccatgtgcgg
    71701 ataaaccacc acatcgcgca gcggcaatac ggggatttca atgcgttcag aacgctcagg
    71761 attcatagag ctctctctta gtttaatttc cgccaggtaa tcagatgaca cgactgtgct
    71821 tcacgccatc tattaacatg tacgtcagta tatggggatg tttcccccac attcaacgcc
    71881 gagaatagag gaaaaattaa aggggagata aaatcccccc tttttggtta actaattgta
    71941 tgggaatggt taattattca ccagatgcct gttgcgcttc cggcttacca taaatcagca
    72001 acggtttgct ttgaccatca attaccgact cgtcgataac cactttttcg acgtcttcca
    72061 tggacggcag atcgtacatg gtatcgagca gtgcggcttc tacgatggaa cgcaggccac
    72121 gggcaccggt tttacgcgcc atcgctttct tagcgatagc atccagcgcc tcgtcacgga
    72181 attccagatc cacgccttcc agattaaaca gcgcctgata ctgcttggtc agggcatttt
    72241 tcggctcttt gaggatctga atcagagctt cttcgctcag ttcattcaac gttgctacaa
    72301 ccggcagacg accaatgaac tcagggataa gaccaaactt gatcagatct tccggttcaa
    72361 cctgcgccag cagctcgcct tcgcttgctt tgtcggactt cgcttttacc gtcgcgccaa
    72421 aaccaatgcc ggagccggtt tctacacggt gggaaatcac tttatccaga ccggcaaacg
    72481 caccgccaca aataaacagg atcttagagg tatcaacctg caagaattcc tgctgcggat
    72541 gtttacgccc accttgcggt ggaacagcag ctaccgtacc ttcgatcagt ttcaacagtg
    72601 cctgctgtac tccttcaccg gaaacgtctc gggtaatgga cgggttgtct gacttacgag
    72661 aaatcttgtc gatttcatcg atgtagacaa tcccgcgctg cgctttctgt acgtcgtaat
    72721 cgcacttttg caacagcttc tgaatgatgt tttcaacgtc ttcgcccaca taaccggctt
    72781 cggtcagcgt ggttgcgtcg gccatggtga acgggacgtc cagcaggcgc gccagcgttt
    72841 cagccagcag cgttttaccg gaaccggtcg gaccgatcag cagaatgtta cttttgccca
    72901 actcgacacc attgctggta tcgccgttgc gcagacgttt gtaatggttg tataccgcga
    72961 ccgccagcac ttttttcgcc tgttcctggc cgataacgta atcgtccagg tggttgcgaa
    73021 tttcatgcgg cgtcggtagc gcactgcgtt cacgatgcgg tgcaacttct ttaatctctt
    73081 cgcgaatgat gtcgttacat aaatcaacac attcgtcgca gatatacacg gatggaccgg
    73141 caatcagctt gcgcacttca tgctggcttt tgccgcaaaa agagcaatac agcaatttgc
    73201 ctgagccatc tttgcgttta tctgtcatgg gtcaaaacct cttctttgtt ctttgtgccg
    73261 cacacgacga cgcaaatgcc attctcaggc gcaagccgct tacagcgttg tgccgcccta
    73321 gataagtata gcggcacagt tgcgcctctg gcatcaatta cgatgggtca gaatcgaatc
    73381 gaccagaccg tattccaccg cttcaggggc ggaaaggaag cgatcgcgct cggtatcacg
    73441 ttcaatctgt tctaatgatt gacccgtatg aagcgccata agttcattca tgcgcccttt
    73501 aactttcaga atttcacggg catgaatttc gatatcggtc gcctggccct ggtagccgcc
    73561 caacggttgg tgaatcatca cgcgcgaatt cggcaggcaa aaacgtttac cttttgcccc
    73621 tgcggtcaac aagaaagcgc ccatcgaggc cgcctggccc atacagatgg tgctgacatc
    73681 aggcttgata aactgcatgg tgtcatagat agacatcccg gcagtgatca ccccgcctgg
    73741 ggagttaatg tacagataga tatctttttc tgggttttcc gcttccagga acagcatctg
    73801 cgccacaatc aggttagcca tgtggtcttc aacctggcca gtcagaaaaa tgacgcgttc
    73861 cttaagtaga cgagaataga tatcaaaaga gcgctcaccg cgtgaggtct gttcaatgac
    73921 catcggcacc agcgccatat ggggtgcaaa gttatctcgt tcgccgctgt atgacatttc
    73981 cgtctcctgg ataaaattga aaaaaacctg ctgtaccgat tgtaacctta tggacggatt
    74041 atcagctagt ccctgtgtta tgggtacggc atcaccatat ttcaagcata acaatctttt
    74101 gttgttacgc taacaccgaa agaggctttt cgcacggctc catgcaaaat tcatgacaaa
    74161 aaaaagccca ccacctggcg gtgacgggcc tttgtgcgaa tttagcgcgt tatgctgcgt
    74221 aaattacgcc tgctggttca tcagctcgtt gaaagtggtt tctttttcag tcactttcgc
    74281 tttcgccagt acagcttcaa cagcctgttc ttccagagca acattgcgca tgttgtccat
    74341 cagttctttg tttttgctgt agaactcgat aacttctttc ggatcttcgt acgcagaagc
    74401 catctcttcg atcaggcctt tcacgcgctc ttcgtcagct ttcagctcgt tggtgcggat
    74461 aacttcgccc agcagcaggc caacaactac gcggcgttta gcctgttctt cgaacagttc
    74521 gcgcggcagt tccagagctt gtttttcgtt gccaccgaaa cgctgtgcag cctggcgacg
    74581 cagaacgtcg atttcgctgt cgatcagcgc agccggtacg tcgatgtcgt tagcttttac
    74641 cagaccttcg atcgcctgag acttaacgcg gttacggatg gcgctcttca gctcgcgctc
    74701 catattttta cgcacttcag cgcgcagacc ttctacggaa ccatcttcaa cgccgaaacg
    74761 tttgatgaat tctgcagtca gttccggcag ttcacgctct tcaactttct tcaggttgat
    74821 agcgaatttc gctgctttac ctttcaggtt ttctgcgtgg tattcttccg ggaaggtcac
    74881 gtcgatggtg aactcttcgc cagctttgtg gcctttgata ccgtcttcaa agcccgggat
    74941 catacgaccc tggcccatcg ccagtacgaa atcagacgct ttaccgcctt cgaactcttc
    75001 gccgtctaca gaaccggtga agtcgatggt tacgcggtct tctgcttcaa cagcgccgtc
    75061 tttttctttc caggtcgcct gctgtttacg cagagtatcc agcatgccgt caacgtcagc
    75121 gtcggtcact tcaacgatcg gtttttcaac ttcgatcgct tccagaccct gcagttcaac
    75181 ttccggataa acttcaaact ctacagagta agtgaagtct tcacccagct tgtattcgcc
    75241 cggaacataa gtcggtgcgc cagccggatt gattttttct ttaatgatgg cgtcaatgaa
    75301 gttacggctc atcaggtcac ccagaacgtc ctggcgtaca gacgcgccat aacgctgagc
    75361 aacgatattc attggcactt tgcctttgcg gaagccgtca atacgtactt ttttcgcaac
    75421 gttgaccagc tcgcttttaa cagcggtctc gatgctgtca gcagcgatag taatcgttac
    75481 acggcggcca aggccttgag tggtttcaac tgaaacttgc atcttgttac ctcaaaaaat
    75541 cacagtgctc ggtcaactct taccccgcaa gcacaggttg ttggcttcgc ggaaccggga
    75601 tgttcttata atcaatcaca ttccctgtta ccagaatcat cccgaaagca ttcaaatagg
    75661 acgcggcatt atagcggcat cacttttatg agtcgagaac ggttattgcg cgctgtcgcg
    75721 tattttttca caattcccgg gcaattttcg tctgaatgct gggcaaaaag cgcaaaatcc
    75781 agacaaaaca aaacggcccg caggccgttt ttcataaaat ctttagcaac atactggaaa
    75841 agcgccgaca gttgcaaatg cgtttttacg cgatgcttcc tgctccacga cagggaggag
    75901 aggcaaagac ggtgtcctgc aacccttccc actccttaat agtgtaagta tgcagagcca
    75961 gcgcatgaac ggtagtagag agttcctccg ctaaagtact gtaaatcatt cgatgacgat
    76021 tcagaaaacg ttcacccgta aaacgatcgc tgaccagcac aactttaaaa tggctttcag
    76081 agccggctgg gacattgtga cgatagcttt catccactac ttcgaggaat acgggttgga
    76141 acgccgccct taatttttct tctatccgct cacgtatcat catgaaatat ctcctccgac
    76201 aacgctgaga tgtcatccat ccctttaaat actagccgct tttaccgttt ccattgcagc
    76261 ttaacaacaa atatttaggt ttcgtcctgg tttttccgtc aagcgtatga agtttactga
    76321 tttaaagatt gaaacacagg cctcgcatca gcgtttcttt taccaggggt gattatttcg
    76381 tcaacatgat gaatttacat gccttaccca cttcccctcg ccgttggcaa tgttatgatg
    76441 gcgggaattt ttaccaaaca cgcttaagag tctctgagag cccgaacatg ttcaaaaaaa
    76501 tcctcttccc gttagttgct ctgtttatgc ttgcaggatg cgcaaaaccg ccaacaacta
    76561 ttgaagtttc cccgacgatt acactgccac agcaggatcc aagcctgatg ggcgtcaccg
    76621 taagcattaa tggtgccgat cagcgtaccg atcaggcgct ggcaaaagtc acccgcgata
    76681 atcaaatcgt taccctgacc gcctcccgcg atctgcgttt cctgctgcaa gaagtgctgg
    76741 aaaaacagat gaccgcgcgt ggttacatgg ttggtccgaa tggcccggtt aatctgcaaa
    76801 tcatcgttag ccaactgtat gctgacgtgt cccagggcaa cgtgcgctac aacatcgcta
    76861 ccaaagcgga tatcgccatc atcgctaccg cgcagaatgg caacaaaatg accaaaaact
    76921 atcgtgccag ctacaacgtt gaaggtgcgt tccaggcctc caacaaaaat atcgccgatg
    76981 cggttaacag tgtgctgacc gataccatcg ctgatatgtc tcaggacacc agcattcacg
    77041 aattcatcaa gcagaacgcg cgttaatttc tgccctctgg cccggtgcaa gccgggcctg
    77101 tagacgccca tgtccagtca atatttacgt atttttcaac agccgcgttc agccatattg
    77161 ctgatcctgg gttttgcttc cgggctaccg ctcgccctga catccggcac cttacaggcc
    77221 tggatgacgg tcgagaatat cgatctcaaa accattggtt tcttctctct ggtaggccag
    77281 gcttacgttt ttaaattcct ctggtcaccg ctgatggacc gctacacgcc cccatttttt
    77341 gggcggcggc gcggttggct gctcgccacg caaatcctgt tattagtcgc cattgcggcg
    77401 atgggttttc tcgaaccagg cacccaactc cgctggatgg cggcgctggc ggtggtgatc
    77461 gctttttgct ctgcctccca ggatattgtc ttcgatgcgt ggaaaaccga tgtgctcccg
    77521 gcagaagaac gtggtgcggg cgcggcaatc agcgtgctgg gttaccgttt agggatgctg
    77581 gtttccggcg gcctagccct gtggctggca gataaatggt tgggctggca gggcatgtac
    77641 tggttgatgg cggcgctgtt gatcccctgt attatcgcga cgttgcttgc accagaacca
    77701 accgacacca ttcctgtgcc caaaacgctg gaacaagcgg ttgttgcacc tctgcgagat
    77761 ttctttggtc gcaataatgc ctggcttatt ttgcttctta tcgtgctgta taagctgggc
    77821 gacgcattcg ccatgagcct gacaaccacg tttttgattc gcggcgtcgg gtttgatgcg
    77881 ggtgaagtag gcgtggtaaa caaaacgctt ggcttactgg cgaccattgt tggcgcattg
    77941 tacggtggga ttttgatgca gcgcctgtcg ctgttccggg cgctgctgat tttcggcatt
    78001 ttacaaggtg cgtctaacgc tggttactgg ctgctgtcga ttactgataa gcatctctac
    78061 agcatgggcg cagccgtctt tttcgaaaac ctctgtggcg ggatgggcac atcagccttt
    78121 gtcgcgctgt taatgacgct gtgtaataag tcattttccg ctactcaatt tgccctgctc
    78181 tcagcgcttt ccgctgtagg gcgagtttat gtcggccccg tggcgggttg gtttgttgaa
    78241 gcacacggct ggtcgacatt ctatctattc tccgtcgccg ctgccgtacc ggggcttatt
    78301 ttgctgctgg tttgccgcca gacgcttgaa tatacacgag taaatgacaa ctttatctcc
    78361 cgtaccgaat atccggcagg ttatgccttt gccatgtgga cactggcggc gggcgtcagc
    78421 ctgttggccg tgtggttact tctgttgacg atggacgcgc tggatttgac gcacttctct
    78481 ttcctgcctg ctctgctgga agtcggggtt ttagtcgccc tttctggcgt cgtgcttggt
    78541 ggtttgctgg attatctggc gctacgaaaa acgcatctga cgtaatctgt aaatattatt
    78601 tagctgtagt aatcactcgc cgtaattatt acggctaaat aaacatcagc atcgcttatt
    78661 aatatttgtt cttttgtgcg gcttagcgtt tggaaattat tggcgccatt tataaattct
    78721 atttttctta cacgattcag ctaatgagtc tttattttct caccacccag ttgtcactct
    78781 aatgataatt atttgttaaa taattgtttt atttcgcatt ggttatacca attgcccgcc
    78841 aagactcctt ctgcactccc cttttgttat aacgcccttt tgcaacagct tcttaaaatc
    78901 aacctgatat gttttgcaac atatgtgacc tgacagccaa atctaagtaa caggaattta
    78961 accatgttta cagtaatgta acctccccgt aaaatgccca cacactttaa acgccaccag
    79021 atcccgtgga attgaggtcg ttaaatgaga ctcaggaaat acaataaaag tttgggatgg
    79081 ttgtcattat ttgcaggcac tgtattgctc agtggctgta attctgcgct gttagatccc
    79141 aaaggacaga ttggtctgga gcaacgttca ctgatactga cggcatttgg cctgatgttg
    79201 attgtcgtta ttcccgcaat cttgatggct gttggtttcg cctggaagta ccgtgcgagc
    79261 aataaagatg ctaagtacag cccgaactgg tcacactcca ataaagtgga agctgtggtc
    79321 tggacggtgc ctatcttaat catcatcttc cttgcggtac tgacctagaa aaccactcac
    79381 gctcttgagc ctagcaagcc gctggcacac gacgagaagc ccattaccat cgaagtggtt
    79441 tccatggact ggaaatggtt cttcatctac ccggaacagg gcattgcaac cgtgaatgaa
    79501 atcgctttcc cggcgaacac tccggtgtac ttcaaagtga cctccaactc cgtgatgaac
    79561 tccttcttca ttccgcgtct gggtagccag atttatgcca tggccggtat gcagactcgc
    79621 ctgcatctga tcgccaacga acccggtact tatgacggta tctccgccag ctacagcggg
    79681 ccgggcttct caggcatgaa gttcaaagct attgcaacac cggatcgcgc cgaattcgac
    79741 caatgggtcg caaaagcgaa acagtcgccg aacaccatgt ctgacatggc tgcgttcgaa
    79801 aaactggccg cgcctagcga atacaaccag gtggaatatt tctccaacgt gaaaccagac
    79861 ttgtttgccg atgtgattaa caagtttatg gctcacggta agagcatgga catgacccag
    79921 ccagaaggtg agcacagtgc acacgaaggt atggaaggca tggacatgag ccacgcggaa
    79981 tccgcccatt aaaggggttg aggaagaata aagatgttcg gaaaattatc acttgatgca
    80041 gtcccgttcc atgaacctat cgtcatggtt acgatcgctg gcattatttt gggaggtctg
    80101 gcgctcgttg gcctgatcac ttacttcggt aagtggacct acctgtggaa agagtggctg
    80161 acctccgtcg accataaacg cctcggtatc atgtatatca tcgtggcgat tgtgatgttg
    80221 ctgcgtggtt ttgctgacgc cattatgatg cgtagccagc aggctcttgc ctcggcgggt
    80281 gaagcgggct tcctgccacc tcaccactac gatcagatct tcaccgcgca cggcgtgatt
    80341 atgatcttct tcgtggcgat gcctttcgtt atcggtctga tgaacctggt ggttccgctg
    80401 cagatcggcg cgcgtgacgt tgcgttcccg ttcctcaaca acttaagctt ctggtttact
    80461 gttgttggtg tgattctggt taacgtttct ctcggcgtgg gcgaatttgc gcagaccggc
    80521 tggctggcct atccaccgct atcgggaata gagtacagtc cgggagtcgg tgttgattac
    80581 tggatatgga gtctccagct atccggtata ggtacgacgc ttaccggtat caacttcttc
    80641 gttaccattc tgaagatgcg cgcaccgggc atgaccatgt tcaagatgcc agtatttacc
    80701 tgggcatcac tgtgcgcgaa cgtactgatt attgcttcct tcccaattct gacggttacc
    80761 gtcgcgttgt tgaccctgga tcgctatctg ggcacccatt tctttaccaa cgatatgggt
    80821 ggcaacatga tgatgtacat caacctgatt tgggcctggg gccacccgga agtttacatc
    80881 ctgatcctgc ctgttttcgg tgtgttctcc gaaattgcgg caaccttctc gcgtaaacgt
    80941 ctgtttggtt atacctcgct ggtatgggca accgtctgca tcaccgtgct gtcgttcatc
    81001 gtttggctgc accacttctt tacgatgggt gcgagcgcga acgtaaacgc cttctttggt
    81061 atcaccacaa tgattatcgc catcccgacc ggggtgaaga tcttcaactg gctgttcacc
    81121 atgtatcagg gccgcatcgt gttccattct gcgatgctgt ggactatcgg ttttatcgtc
    81181 accttctcgg tgggcggtat gactggcgtg ctgctggcag taccgggcgc ggacttcgtt
    81241 ctgcataaca gcctgttcct gattgcgcac ttccataacg tgatcatcgg cggcgtggtc
    81301 ttcggctgct tcgcagggat gacctactgg tggcctaaag cgttcggttt caaactgaac
    81361 gaaacctggg gtaaacgcgc gttctggttc tggatcatcg gcttcttcgt tgcctttatg
    81421 ccgctgtatg cgctgggctt catgggcatg acccgtcgtt tgagccagca gattgacccg
    81481 cagttccaca ccatgctgat gattgcagcc agcggtgcag tactgattgc gctgggtatt
    81541 ctctgcctcg ttattcagat gtacgtttct attcgcgacc gcgaccagaa ctgtgacctg
    81601 actggcgacc cgtggggtgg ccgtacgctg gagtgggcaa cctcttcccc gcctccgttc
    81661 tataactttg ccgtagtgcc gcacgttcac gaacgtgatg cattctggga aatgaaagag
    81721 aaaggcgaag cgtataaaaa gcctgaccac tatgaagaaa ttcatatgcc gaaaaacagc
    81781 ggtgcaggta tcgtcattgc agctttctcc accatcttcg gtttcgccat gatctggcat
    81841 atctggtggc tggcgattgt tggcttcgca ggcatgatca tcacctggat cgtgaaaagc
    81901 ttcgacgagg acgtggatta ctacgtgccg gtggcagaaa tcgaaaaact ggaaaaccag
    81961 catttcgatg agattactaa ggcagggctg aaaaatggca actgatactt tgacgcacgc
    82021 gactgcccac gcgcacgaac acgggcacca cgatgcaggc ggaaccaaaa tcttcggatt
    82081 ttggatctac ctgatgagcg actgcattct gttctcaatc ttgtttgcta cctatgccgt
    82141 tctggtgaac ggcaccgcag gcggcccgac aggtaaggac attttcgaac tgccgttcgt
    82201 tctggttgaa actttcttgc tgttgttcag ctccatcacc tacggcatgg cggctatcgc
    82261 catgtacaaa aacaacaaaa gccaggttat ctcctggctg gcgttgacct ggttgtttgg
    82321 tgccggattt atcgggatga aaatctatga attccatcac ctgattgtta acggcatggg
    82381 tccggatcgc agcggcttcc tgtcagcgtt ctttgcgctg gtcggcacgc acggtctgca
    82441 cgtcacttct ggtcttatct ggatggcggt gctgatggtg caaatcgccc gtcgcggcct
    82501 gaccagcact aaccgtaccc gcatcatgtg cctgagcctg ttctggcact tcctggatgt
    82561 ggtttggatc tgtgtgttca ctgttgttta tctgatgggg gcgatgtaat gagtcattct
    82621 accgatcaca gcggcgcgtc ccatggcagc gtaaaaacct acatgacagg ctttatcctg
    82681 tcgatcattc tgacggtgat tccgttctgg atggtgatga caggggctgc ctctccggcc
    82741 gtaattctgg gaacaatcct ggcaatggca gtggtacagg ttctggtgca tctggtgtgc
    82801 ttcctgcaca tgaataccaa atcagatgaa ggctggaaca tgacggcgtt tgtcttcacc
    82861 gtgctaatca tcgctatcct ggttgtaggc tccatctgga ttatgtggaa cctcaactac
    82921 aacatgatga tgcactaaga gcggcggtta tgatgtttaa gcaatacctg caagtaacga
    82981 aaccaggcat catctttggc aacctgatct cggtgattgg gggattcctg ctggcctcaa
    83041 agggcagcat tgattatccc ctgtttatct acacgctggt tggggtgtca ctggttgtgg
    83101 cgtcgggttg tgtgtttaac aactacatcg acagggatat cgacagaaag atggaaagga
    83161 cgaagaatcg ggtgctggtt aaaggcctga tctctcctgc tgtctcgctg gtgtacgcca
    83221 ccttgctggg tattgctggc tttatgctgc tgtggtttgg cgcgaatccg ctggcctgct
    83281 ggctgggggt gatgggcttt gtggtttatg tcggcgtcta tagcctgtac atgaaacgcc
    83341 actcagtcta cggcacattg attggttcgc tctccggcgc tgcgccgccg gtgattggct
    83401 actgtgcagt aaccggcgag ttcgatagcg gcgcagcgat cctgctggct atcttcagcc
    83461 tgtggcagat gcctcactcc tatgccatcg ccattttccg ctttaaggat taccaggcgg
    83521 caaacattcc ggtattgcca gtggtaaaag gcatttcggt ggcgaagaat cacatcacgc
    83581 tgtatatcat cgcctttgcc gttgccacgc tgatgctctc tcttggcggt tacgctgggt
    83641 ataaatatct ggtggtcgcc gcggcggtta gcgtctggtg gttaggtatg gctctgcgcg
    83701 gttataaagt tgctgatgac agaatctggg cgcgcaagct gttcggcttc tctatcatcg
    83761 ccatcactgc cctctcggtg atgatgtccg ttgattttat ggtaccggac tcgcatacgc
    83821 tgctggctgc tgtgtggtaa caaaacctct ctattaaaaa ggtgctacgg cacctttttt
    83881 attgtctgag atcagagata cggctaataa ccgtatctct tgacagagtg agcagatgca
    83941 cataaattaa tcatacggtt gatagccgta ttatgtatga gatcgactcc ctacgatttc
    84001 attcagttaa tcaaagaagt cataatgcct ggaaaaacag ccgctctata cgatgtcgat
    84061 aaaacgctga aaaatgcacg ggtcgaactc aaaactagtc ccgacgccaa aaacaaactt
    84121 cgtgaagctg cgcaagccgt tggggtagat ttaagcgctt ttattttgag cgttgctatg
    84181 gaacgcgccg aaagtgtgct tgataaccaa cgtcgccgtg agctttcgaa tcaaagctgg
    84241 gaactgatga accaactcat cgctgaacct gctcaaccga cgctcgccct caaggcgtta
    84301 atgaaaagga aaaacagcga tggtcgacaa gcatgaagag attactctgc ccatagtcct
    84361 ctcctgtaat tatcagtctg atattactta tcctgggcaa aaacagtttg attgcggtaa
    84421 ccctgttatc gataaatttg tacgcgcatc gctaaagaaa agtgtgcgta atagcgactg
    84481 tgcggctaaa gcacttattg acagacaaag tggtgaactg attggcatct gtacttttac
    84541 ggcatattcg ctggaaagac aacgcgtttc tggcgtcctt cagggttcac aaccttcaga
    84601 aattggtgtt gtcagattag tcatgttggg gggagcgcgg aagtatcaaa agcggggctt
    84661 tggtcaggac ctactatgtg
//
LOCUS       DADLSA010000003        82838 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.003, whole genome shotgun sequence.
ACCESSION   DADLSA010000003 DADLSA010000000
VERSION     DADLSA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 82838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.003"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..810)
                     /gene="ugpC"
                     /locus_tag="JN691_000189"
     CDS             complement(<1..810)
                     /gene="ugpC"
                     /locus_tag="JN691_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAY7241730.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLK"
     gene            complement(824..1483)
                     /gene="pgmB"
                     /locus_tag="JN691_000190"
     CDS             complement(824..1483)
                     /gene="pgmB"
                     /locus_tag="JN691_000190"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAY7241731.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1480..3747)
                     /gene="ycjT"
                     /locus_tag="JN691_000191"
     CDS             complement(1480..3747)
                     /gene="ycjT"
                     /locus_tag="JN691_000191"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HAY7241732.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGYVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(3744..4799)
                     /locus_tag="JN691_000192"
     CDS             complement(3744..4799)
                     /locus_tag="JN691_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY7241733.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCGSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAWEMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQLIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4809..5597)
                     /locus_tag="JN691_000193"
     CDS             complement(4809..5597)
                     /locus_tag="JN691_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAY7241734.1"
                     /translation="MKIGTQNQAFFPENIRERFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5615..6667)
                     /locus_tag="JN691_000194"
     CDS             complement(5615..6667)
                     /locus_tag="JN691_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAY7241735.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6698..7540)
                     /locus_tag="JN691_000195"
     CDS             complement(6698..7540)
                     /locus_tag="JN691_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY7241736.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(7527..8408)
                     /locus_tag="JN691_000196"
     CDS             complement(7527..8408)
                     /locus_tag="JN691_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY7241737.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8429..9712)
                     /locus_tag="JN691_000197"
     CDS             complement(8429..9712)
                     /locus_tag="JN691_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7241738.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNRQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLASLTITAGQKTEAAEKFVTFMEQADNIADWVMMSPG
                     AALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDVTGS
                     GVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9726..11403)
                     /locus_tag="JN691_000198"
                     /pseudo
     CDS             complement(9726..11403)
                     /locus_tag="JN691_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(11615..11962)
                     /gene="pspE"
                     /locus_tag="JN691_000199"
     CDS             complement(11615..11962)
                     /gene="pspE"
                     /locus_tag="JN691_000199"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAY7241739.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIVTAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(12005..12226)
                     /gene="pspD"
                     /locus_tag="JN691_000200"
     CDS             complement(12005..12226)
                     /gene="pspD"
                     /locus_tag="JN691_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAY7241740.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(12235..12594)
                     /gene="pspC"
                     /locus_tag="JN691_000201"
     CDS             complement(12235..12594)
                     /gene="pspC"
                     /locus_tag="JN691_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAY7241741.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12594..12818)
                     /gene="pspB"
                     /locus_tag="JN691_000202"
     CDS             complement(12594..12818)
                     /gene="pspB"
                     /locus_tag="JN691_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAY7241742.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12872..13540)
                     /gene="pspA"
                     /locus_tag="JN691_000203"
     CDS             complement(12872..13540)
                     /gene="pspA"
                     /locus_tag="JN691_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAY7241743.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDKAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13707..14684
                     /gene="pspF"
                     /locus_tag="JN691_000204"
     CDS             13707..14684
                     /gene="pspF"
                     /locus_tag="JN691_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAY7241744.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14804..16069)
                     /gene="puuE"
                     /locus_tag="JN691_000205"
     CDS             complement(14804..16069)
                     /gene="puuE"
                     /locus_tag="JN691_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HAY7241745.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(16107..17387)
                     /locus_tag="JN691_000206"
     CDS             complement(16107..17387)
                     /locus_tag="JN691_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY7241746.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(17389..18876)
                     /gene="puuC"
                     /locus_tag="JN691_000207"
     CDS             complement(17389..18876)
                     /gene="puuC"
                     /locus_tag="JN691_000207"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HAY7241747.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(19026..19583)
                     /gene="puuR"
                     /locus_tag="JN691_000208"
     CDS             complement(19026..19583)
                     /gene="puuR"
                     /locus_tag="JN691_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HAY7241748.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19610..20374)
                     /gene="puuD"
                     /locus_tag="JN691_000209"
     CDS             complement(19610..20374)
                     /gene="puuD"
                     /locus_tag="JN691_000209"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HAY7241749.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            20586..22004
                     /locus_tag="JN691_000210"
     CDS             20586..22004
                     /locus_tag="JN691_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAY7241750.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGYYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRRRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            22153..22317
                     /locus_tag="JN691_000211"
     CDS             22153..22317
                     /locus_tag="JN691_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7241751.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            22307..23692
                     /gene="puuP"
                     /locus_tag="JN691_000212"
     CDS             22307..23692
                     /gene="puuP"
                     /locus_tag="JN691_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HAY7241752.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23826..24071
                     /locus_tag="JN691_000213"
     CDS             23826..24071
                     /locus_tag="JN691_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAY7241753.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDKIAEFLNQWGNE"
     gene            24384..26027
                     /gene="sapA"
                     /locus_tag="JN691_000214"
     CDS             24384..26027
                     /gene="sapA"
                     /locus_tag="JN691_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAY7241754.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            26024..26989
                     /gene="sapB"
                     /locus_tag="JN691_000215"
     CDS             26024..26989
                     /gene="sapB"
                     /locus_tag="JN691_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HAY7241755.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26976..27866
                     /gene="sapC"
                     /locus_tag="JN691_000216"
     CDS             26976..27866
                     /gene="sapC"
                     /locus_tag="JN691_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAY7241756.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27866..28858
                     /gene="sapD"
                     /locus_tag="JN691_000217"
     CDS             27866..28858
                     /gene="sapD"
                     /locus_tag="JN691_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAY7241757.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28860..29666
                     /gene="sapF"
                     /locus_tag="JN691_000218"
     CDS             28860..29666
                     /gene="sapF"
                     /locus_tag="JN691_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAY7241758.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29734..30087
                     /locus_tag="JN691_000219"
     CDS             29734..30087
                     /locus_tag="JN691_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HAY7241759.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            30455..31243
                     /gene="fabI"
                     /locus_tag="JN691_000220"
     CDS             30455..31243
                     /gene="fabI"
                     /locus_tag="JN691_000220"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAY7241760.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            31387..32514
                     /locus_tag="JN691_000221"
     CDS             31387..32514
                     /locus_tag="JN691_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HAY7241761.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            32582..34516
                     /locus_tag="JN691_000222"
     CDS             32582..34516
                     /locus_tag="JN691_000222"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAY7241762.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(34594..34696)
                     /locus_tag="JN691_000223"
                     /pseudo
     CDS             complement(34594..34696)
                     /locus_tag="JN691_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34750..36735
                     /gene="pdeR"
                     /locus_tag="JN691_000224"
                     /pseudo
     CDS             34750..36735
                     /gene="pdeR"
                     /locus_tag="JN691_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            36883..37056
                     /locus_tag="JN691_000225"
     CDS             36883..37056
                     /locus_tag="JN691_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7241763.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            37146..37895
                     /locus_tag="JN691_000226"
     CDS             37146..37895
                     /locus_tag="JN691_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7241764.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            38164..38382
                     /gene="osmB"
                     /locus_tag="JN691_000227"
     CDS             38164..38382
                     /gene="osmB"
                     /locus_tag="JN691_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAY7241765.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38508..38834)
                     /gene="yciH"
                     /locus_tag="JN691_000228"
     CDS             complement(38508..38834)
                     /gene="yciH"
                     /locus_tag="JN691_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAY7241766.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38834..39571)
                     /gene="pyrF"
                     /locus_tag="JN691_000229"
     CDS             complement(38834..39571)
                     /gene="pyrF"
                     /locus_tag="JN691_000229"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAY7241767.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39764..40933)
                     /gene="lapB"
                     /locus_tag="JN691_000230"
     CDS             complement(39764..40933)
                     /gene="lapB"
                     /locus_tag="JN691_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAY7241768.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40940..41248)
                     /gene="lapA"
                     /locus_tag="JN691_000231"
     CDS             complement(40940..41248)
                     /gene="lapA"
                     /locus_tag="JN691_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAY7241769.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(41397..42161)
                     /gene="pgpB"
                     /locus_tag="JN691_000232"
     CDS             complement(41397..42161)
                     /gene="pgpB"
                     /locus_tag="JN691_000232"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAY7241770.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKIHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            42331..42921
                     /gene="ribA"
                     /locus_tag="JN691_000233"
     CDS             42331..42921
                     /gene="ribA"
                     /locus_tag="JN691_000233"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAY7241771.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHIALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(42985..45660)
                     /gene="acnA"
                     /locus_tag="JN691_000234"
     CDS             complement(42985..45660)
                     /gene="acnA"
                     /locus_tag="JN691_000234"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAY7241772.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYVLAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(45824..45919)
                     /locus_tag="JN691_000235"
     CDS             complement(45824..45919)
                     /locus_tag="JN691_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7241773.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(46033..46200)
                     /locus_tag="JN691_000236"
     CDS             complement(46033..46200)
                     /locus_tag="JN691_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7241774.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(46203..46367)
                     /locus_tag="JN691_000237"
     CDS             complement(46203..46367)
                     /locus_tag="JN691_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAY7241775.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(46662..47636)
                     /gene="cysB"
                     /locus_tag="JN691_000238"
     CDS             complement(46662..47636)
                     /gene="cysB"
                     /locus_tag="JN691_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAY7241776.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(47846..50443)
                     /gene="topA"
                     /locus_tag="JN691_000239"
     CDS             complement(47846..50443)
                     /gene="topA"
                     /locus_tag="JN691_000239"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAY7241777.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            50823..51074
                     /locus_tag="JN691_000240"
     CDS             50823..51074
                     /locus_tag="JN691_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAY7241778.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(51110..52159)
                     /gene="sohB"
                     /locus_tag="JN691_000241"
     CDS             complement(51110..52159)
                     /gene="sohB"
                     /locus_tag="JN691_000241"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAY7241779.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            52379..53137
                     /locus_tag="JN691_000242"
     CDS             52379..53137
                     /locus_tag="JN691_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAY7241780.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            53134..53724
                     /gene="cobO"
                     /locus_tag="JN691_000243"
     CDS             53134..53724
                     /gene="cobO"
                     /locus_tag="JN691_000243"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAY7241781.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACRKVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(53753..54625)
                     /gene="rluB"
                     /locus_tag="JN691_000244"
     CDS             complement(53753..54625)
                     /gene="rluB"
                     /locus_tag="JN691_000244"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAY7241782.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(54726..55346)
                     /locus_tag="JN691_000245"
     CDS             complement(54726..55346)
                     /locus_tag="JN691_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAY7241783.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(55343..56224)
                     /gene="yciV"
                     /locus_tag="JN691_000246"
     CDS             complement(55343..56224)
                     /gene="yciV"
                     /locus_tag="JN691_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAY7241784.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            56362..56406
                     /gene="trpL"
                     /locus_tag="JN691_000247"
     CDS             56362..56406
                     /gene="trpL"
                     /locus_tag="JN691_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HAY7241785.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            56498..58060
                     /gene="trpE"
                     /locus_tag="JN691_000248"
     CDS             56498..58060
                     /gene="trpE"
                     /locus_tag="JN691_000248"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAY7241786.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            58060..59655
                     /gene="trpD"
                     /locus_tag="JN691_000249"
     CDS             58060..59655
                     /gene="trpD"
                     /locus_tag="JN691_000249"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAY7241787.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAATLVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            59659..61017
                     /gene="trpCF"
                     /locus_tag="JN691_000250"
     CDS             59659..61017
                     /gene="trpCF"
                     /locus_tag="JN691_000250"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAY7241788.1"
                     /translation="MQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGL
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            61029..62222
                     /gene="trpB"
                     /locus_tag="JN691_000251"
     CDS             61029..62222
                     /gene="trpB"
                     /locus_tag="JN691_000251"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAY7241789.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPSGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            62222..63028
                     /gene="trpA"
                     /locus_tag="JN691_000252"
     CDS             62222..63028
                     /gene="trpA"
                     /locus_tag="JN691_000252"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAY7241790.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGIFAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            63409..63588
                     /locus_tag="JN691_000253"
     CDS             63409..63588
                     /locus_tag="JN691_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAY7241791.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            63674..64174
                     /locus_tag="JN691_000254"
     CDS             63674..64174
                     /locus_tag="JN691_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY7241792.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            64220..64726
                     /locus_tag="JN691_000255"
     CDS             64220..64726
                     /locus_tag="JN691_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY7241793.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(64786..65424)
                     /gene="ompW"
                     /locus_tag="JN691_000256"
     CDS             complement(64786..65424)
                     /gene="ompW"
                     /locus_tag="JN691_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAY7241794.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            65664..65753
                     /locus_tag="JN691_000257"
                     /pseudo
     CDS             65664..65753
                     /locus_tag="JN691_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            65781..66524
                     /locus_tag="JN691_000258"
     CDS             65781..66524
                     /locus_tag="JN691_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAY7241795.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            66554..67093
                     /locus_tag="JN691_000259"
     CDS             66554..67093
                     /locus_tag="JN691_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAY7241796.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            67198..67596
                     /gene="yciA"
                     /locus_tag="JN691_000260"
     CDS             67198..67596
                     /gene="yciA"
                     /locus_tag="JN691_000260"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAY7241797.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(67636..68364)
                     /gene="tonB"
                     /locus_tag="JN691_000261"
     CDS             complement(67636..68364)
                     /gene="tonB"
                     /locus_tag="JN691_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAY7241798.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            68579..68875
                     /locus_tag="JN691_000262"
     CDS             68579..68875
                     /locus_tag="JN691_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HAY7241799.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            69175..70428
                     /gene="kch"
                     /locus_tag="JN691_000263"
     CDS             69175..70428
                     /gene="kch"
                     /locus_tag="JN691_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAY7241800.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(70486..70659)
                     /locus_tag="JN691_000264"
     CDS             complement(70486..70659)
                     /locus_tag="JN691_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAY7241801.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            70802..72262
                     /gene="cls"
                     /locus_tag="JN691_000265"
     CDS             70802..72262
                     /gene="cls"
                     /locus_tag="JN691_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAY7241802.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            72297..72626
                     /locus_tag="JN691_000266"
     CDS             72297..72626
                     /locus_tag="JN691_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAY7241803.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(72679..73683)
                     /gene="oppF"
                     /locus_tag="JN691_000267"
     CDS             complement(72679..73683)
                     /gene="oppF"
                     /locus_tag="JN691_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAY7241804.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQEPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(73680..74693)
                     /gene="oppD"
                     /locus_tag="JN691_000268"
     CDS             complement(73680..74693)
                     /gene="oppD"
                     /locus_tag="JN691_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAY7241805.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGAATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(74705..75613)
                     /gene="oppC"
                     /locus_tag="JN691_000269"
     CDS             complement(74705..75613)
                     /gene="oppC"
                     /locus_tag="JN691_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAY7241806.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAISGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(75628..76548)
                     /gene="oppB"
                     /locus_tag="JN691_000270"
     CDS             complement(75628..76548)
                     /gene="oppB"
                     /locus_tag="JN691_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAY7241807.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(76634..78265)
                     /gene="oppA"
                     /locus_tag="JN691_000271"
     CDS             complement(76634..78265)
                     /gene="oppA"
                     /locus_tag="JN691_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAY7241808.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTAGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(79003..79650)
                     /gene="ychE"
                     /locus_tag="JN691_000272"
     CDS             complement(79003..79650)
                     /gene="ychE"
                     /locus_tag="JN691_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAY7241809.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            80127..82802
                     /gene="adhE"
                     /locus_tag="JN691_000273"
                     /gene_synonym="adhC"
     CDS             80127..82802
                     /gene="adhE"
                     /locus_tag="JN691_000273"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAY7241810.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAEKSA"
BASE COUNT        20137 a        21479 c        20473 g        20749 t
ORIGIN      
        1 tttcagaacc gctaatttct cttcgggaat ggttaattta agcgtttccg taacgaattt
       61 atcgccatcg atcgtgccgc gaataaaatt catcgctggt gatccaataa atccggcaac
      121 aaacatattc gcgggttggt tataaacggt tttcggcgca cctacttgct gaacaatccc
      181 gtctttcata atcacaatcc gcgtcgccat ggtcatcgct tcggtctgat cgtgggtcac
      241 gtagatcatg gtggtgttca gtttctgatg cagcttgctg atctctgcgc gcatttgtac
      301 gcgcagcttg gcatcaaggt tagagagcgg ttcatccatt aaaaacacgc ccgcttcgcg
      361 cacaatcgcc cgcccgagcg ccacacgctg gcgttgcccg ccggaaagcg cgcccggctt
      421 gcgtttcagg tactcacgca ggccgagaat ttgcgccgcc cagttcaccc gctcatcaat
      481 cacctctttg gcgatttttt gcatcttcag gccaaacgcc atgttgtcgt aaaccgtcat
      541 atgcggatac aacgcgtagt tctggaacac catcgcgata ttgcgtgctt tggctggaac
      601 gtcattcatt cgtttgccgt cgatcaacag atcgccgccg ctgatctcct caagcccggc
      661 aatcatgcgc agggtggtcg atttaccgca gcccgacggg ccgacaaata cgatgaactc
      721 tttatcggca atttccaggt tgaagtcctt caccacatgc acctggttat cgtagatttt
      781 ttgaatatgt tgtaacgaaa gctgagccat gttgattcct ttgctatacg ttttgccaga
      841 aggccgataa ccgcgaccag gtcagggagt ccgttgaggg caacagtaat tgcgccccgg
      901 ttaagcccgc accgatcccc accgagcgca taccgctggc gttaatggcg tcaatgcccg
      961 cctgcgcatc ttcaatgccg atacatgcct gcggcggcac gcccagcccg gcacaggcag
     1021 cgagaaagat ttccggatcc ggtttcgagt ttttaagttg ggaagcatcc gcgcagaagg
     1081 tgaaaaactc gcgcagctcc agcgccgcta aaatcgtcgg cgcattcagg gagacggaag
     1141 ccagccctac cggaatctgc tgtgcacgga gctctgccag caaattgcga ataccgggca
     1201 gcacagcatt aaccgtcagc tcgcgcagtg agtggacgta aagcagattt ttgcgatacg
     1261 ccagttgcgc ccgctcctgc gggttaaagt cgccctcttt gcccccgtgt tgcagaatgc
     1321 gccgcagaga ctcatcgcgg ctgatccctt ttagagattc gttaaactgc gcatcaatac
     1381 tgatgccaat ttcagcggca atctgctgcc acgcctggaa atgcagatgc gcggtatcgg
     1441 tgattacacc atccagatcg aaaattaccc cttgcagttt cattcatcct cctgatgcgt
     1501 ggtagcggtc ccattgaagg gcaaaataaa atcacccaaa cagaaaacag attcttcagc
     1561 cacggatata agctgaccgt tcaaacgcag tgaaacgggc gcagaagttc gaatcgcaat
     1621 acgctgcgcg tcgagagtga cctgtaattc gcagccctgc cagaacagag ggaaagacaa
     1681 ctgttgccac tgctcaggta acgccggatt tagatgcaat tcaccgtcac gcacgctcac
     1741 cccggcaaaa ccctgaatcg cccccagcca gatagcgcca gttgcggcag catggatacc
     1801 atcatcacaa ctatgcggat cagcaccaag atcgatttca gtcccctcgc gccagaactg
     1861 ataactttgg gtcagcaggc cacagcgtgc agcaacaatg ccgtggattg ctttacttaa
     1921 tgacgagtcg tgaatagtgc gcggttcata aaattgcaga ttggcaagac acgatgccga
     1981 tgagaactgc tctggcagca tgtaattgag catcaccaca tcagcttgtt tgaggatctg
     2041 catctcgttc acttctgcgc gtgaataatc cagcagaatg gtttgcttcc ccgccgccgc
     2101 tttgtatttc gccagattaa tcgccggctt agtcataaac gaatcatcct gcggcaaaac
     2161 accgtcgggc tgaatttctg gcagccgcag ttctttaagg aacatttcgg cacgatgaat
     2221 aaacgcgtcg tcactacagc caaactggcg ggcaatactc agcgcctgct ggacgttgta
     2281 atacaccata tagctggtga aggcgttatt attgacatgt tcggtatatt cgtctggccc
     2341 aataacatca tgaatttcca gacggtcgtt aaccctcacc gcgcggctaa tccagaactt
     2401 tgcagtttcc agaagtagcg ccatgccttc atgcgcaatg aaactttcat cccccgtggt
     2461 ctgccagtat tgaataaccg cccaggcgat atcggccacc agatgatgtt ccgcctgcgc
     2521 cgaggccact ttttgccgca gcccggtgcg aatgttaatg gcggcaaatt ccggcgtctc
     2581 ttcttcgccg ctgcgcgcgc tttcccacgg aaatagcgcg ccctgccagc cgttgcgtcg
     2641 cgctttctcc tgcgcgcctg gcaagttgtg ccagcgataa cgcagtaaac ttcgggcaac
     2701 cgtcggatcg ctaaacagat ggaacggcag caaaaatact tctgtatccc agaaaacgta
     2761 gcctttgtag ccttcgccgg ttaagccttt tgccgcaata ctgctgcgct cgtcgtgagc
     2821 cggcgtcatg atacgcagat gataaagcgc ataatctaac gcttgctgat cgtgcgcatc
     2881 gccgccattt accgtgatac gacgtttctg ccaccattga cgccagtttt ctgttgatgc
     2941 tgcaagatgt tggtcgtaac tctgttgcgc gcacatttca agctggcgaa gcgacacgct
     3001 gccccactcg tctaaagcag cttgcctgtc atcccgccag tcgatccaca ccagtttttg
     3061 caacgtcact gtctcgcctg catgaagctg cgcactggta tgttgcagta aacggcgctc
     3121 tttggcggta tagcattgct gcacatcacc gctcacctgg cagcaacaac tgatggccac
     3181 atcactgcgc ccatcctggg tggtgtagat cccctgcatc aaatgctgac caaacacccg
     3241 cacctgggtt tcgtcgagat gctggcgacc gtggttggtt tgcgtggcgt caatgcctgt
     3301 tgaaatcagc actgacgcgt cggcgtccag tggcgtaata gtgatttcca gcgcaatgag
     3361 cggcagttgg tctgccgaaa caaagcgacg gctggcgatg gtgtaacctg cgccgttgct
     3421 ggtacgccag acaacgctac ggcgtaattc tccactggca aagtcaagtt cacgctgcca
     3481 ggcttcgcgg gataacgaga aaacctcacc atttatggca atctccatcc ccaagatatc
     3541 aggcagattc accagttcgt tgatttcacc ttttcccgcc cgatgataca gccccgccag
     3601 atacatccct cgcgtctggc gcgtgtaatc ttcttcatgg ctggcgcgaa gcccaagata
     3661 gccgttcccc tgcgccatca gcgatgcata cttgttcagg gtatgctggc tgaaatgggg
     3721 ttctgataac gttactggcc tggtcataat tccacacgcg tccctgtttc tgcggattga
     3781 tataacgccg ccaccagttg ctggatgatg tacccctgct cggcgtcggc tatcatcacg
     3841 ggcttgccct gtacgtgatt gataaaggct tccatgctgc gcaaatggcg gttgtcgtct
     3901 gccatttccc gttgcatcag cgtcattaat tcaccgttgt tatcggtgta gatatgtgct
     3961 ggaaacagcg tcgcaccggc tttatcacca caaaagctga cgttcataat caactgttcg
     4021 cggatgttga gtgcaaatga cgtttccagc cacagaatgc cgccgttatg aaattcaatg
     4081 gtgccaaaca gcgaatcttc gacgctgtaa gttgccggat cccactcgcc aaattgacca
     4141 cagctctttt gcgtgccgat cttttgaaaa ctatgcgcat tcacgctttt caccgccgga
     4201 aagcccagca catacatcgc agcatccagc atatgaatgc cgatgtcgat cagcgggcca
     4261 ccaccctgca gttctttatt ggtaaagaca ccccagccgg gaacgccgca gcgacgcagg
     4321 gcgcgagcgg tggtaacgta aatctctccc aaaacgccgt tggtcacctg ttcacgcagc
     4381 tgttgcgtat cgagcgcaaa acgatggtga aagtcgtagg ccagcacctt gcccagtttg
     4441 cgcgcggtat cgcacatttc ccacgcctgt tctggcgtca tggcgggcgg tttttcgcac
     4501 atcacatggc agcctgcttc cagtgccatc agggtatgtt cgtaatgaaa acggttaggt
     4561 gagcagacgc taaccacatc aggtttcacc gccagcagca tggcctgcgg atcgtcccac
     4621 acggatgcat tcccgtattt ttctgccagc gcctgcgcct gggaaaggcg gctgccacag
     4681 acagccacca gttccagatc gttgcgggtg cagtagtacg aagcatgaac cttatccgcc
     4741 acctggcccg cgcctattat cgcgacccgc agcggagagc ttgtcattgc acttttcaca
     4801 aagacctctt agcaggtacg caaccaggcc aacgaatcac ggtacgcctg ggcaggatct
     4861 tctgcccgga tacgcccttc atacaccaca taaccctgat agttatcggc gcgcagctgt
     4921 tcaaacagcg cgtggaaatc gagggtgcca ctgcccggct ggtaacgatg gttatcggca
     4981 atatgcacat gaccgagcaa gtcacggttg tcgtgcagcg cctgcgccag gttatcttct
     5041 tcgatgttca tgtgatagaa atcgccgata atctgcacat gtttaagatt gttttcgacg
     5101 atgtaacgac gagcatcggc gagggtgttg atcatatgat cctgatagcg gtttaacggt
     5161 tcgagataca ccacggttcc ggtacgcgcg gcgacctgtt ccagtacgcg cagggaatca
     5221 ctcaccattt tgcggtcgcc gtccaggcta cgcggcgagg tcatcggcgg taagcggaag
     5281 gtaaacatgc cccacgctgc cggaacgacg atacctttac cccccacttc tgccagcgcc
     5341 tcgagaatgc gttcgatctg ctttaagcca ttaagacgac gctcttcgat aaagtcacca
     5401 atccagccgt catagccgcc gcaggcggtg gtcactggta aaccggtttc tttgatcgcc
     5461 gctttgactt cttcgatgtt gttgaccagc agtttgccgt caatctcaaa accatcgaag
     5521 cccatctctt tgatataacg aaatctctcg cggatatttt ccggaaagaa tgcctgattc
     5581 tgtgtgccga ttttcatttt ttcattcctt tgaattaaaa cgtaacgccc attttgatgc
     5641 tctgttccgg atgctgatcg acatactgca tgtagctttc cgggctgttg gcaaaggtca
     5701 ctaccgggtc gattaaatct tcgcaattga gataaccgtt catcagcagt tcccagcagg
     5761 tttcttcaat acgcttacgg ctccagcgcg gataatccgg gttcggttcg ctgcacgcgc
     5821 gggagaaaac aattttggcg ttattgaaat gcgcttcgcg tccgaggtta aaaccttcag
     5881 caaatggttt ggcaaacgcg acataggaga tggtgccgcc gtaggccagg ccgcgcagcg
     5941 ccgattgcag cgcgtcggcg taaccgctgg tttcgataat cacatcggcc ccctgcttgc
     6001 cggtcagcgt tttgatctct ttgcctacgt cagtgccaat ggggttaagg cagaaatcag
     6061 caccgtggcg acgggcaata tcacagcgat gggcgatagg atcgacgcca attaccaccg
     6121 atgcgccagc gcgtttcgcc agttggatgg caatttgacc gatcgcgcca agccctacca
     6181 ccacgacaaa atcccctacg cgtacgttgg catcgcgcac gccgctcatg gcaaactgcg
     6241 ccgggtcgta acagacggcg tttttccagg agctgccttg cggcattttg cgcagcttgt
     6301 agttattcac tgcgttaatg atgaccgtct cggagagcgg gccgtagccg catacgctgt
     6361 cgcccaccgc gtagtctgta acgtcgctgc cgcactcgat aatgtcgcca accaccatgt
     6421 tgccaagctg gaatttgcca aactcaatgc cgcgcggcgc atctgccgga cgcggggtga
     6481 acatctgcca ctcgccgtta aagtcttcat cgataaacgg gctggcggcg cggaagtcga
     6541 ccacttccgt tccgtgtttc ggtgcgccga aacgcacgcg gatcttcact tcattggcta
     6601 aaatcgcccg atcttcatac tcaaccagcg cagcaacacg cggtgctgtg gctactaact
     6661 ttttcatgta aaactccttg taaagaaact ggccgcttta gcccttcacg ccaccggcgg
     6721 tcaaaccact cttaataaaa cgttccgaaa gcgcatacat aatcaccacc ggcaatgcgg
     6781 tcaccagcga ggccgccatc atccgtcccc agatgtaatc aggcgtactg aacagcgcgt
     6841 tcaggcccac tggtaaggtg aaattgctgg cgctggagag gaaaatcgac gcaaacagat
     6901 agtcgttcca cgccaccata aagcagtaga cgaacaccga tatcagcccg gacatcgcca
     6961 ggggcacggt gatacggaag atgatttgca gacggttgag gccatccatc atcgccgctt
     7021 cttcgatctc atcagggatg gtgtcgaagt aacttttcag cataaacacg gcggtgggca
     7081 gggtttgcgt caccatggta atgatcagcg ccatctcggt gtcgtaaata ccaagcgcgg
     7141 tgatgatttt gaacagaggc accaccagca aaatgccaga gaacatatac accgtgtaaa
     7201 agctggcgtt gatggtcatc cggcctttaa agcgcaggcg ggaaagcgcg taagctccga
     7261 gaatgccgag aaataccgcc accacggagg aaaccaccga caccaccatg ctgttacgga
     7321 agtagtcgac aaacgggaaa atcatcgggt taaaaatgtc gacgtaatgc tccagcgtcc
     7381 actgttgcgg cagcagcgta ggatgcagcg atatcgcctc tttcgcgctt ttgaacgagg
     7441 tcatcagcat cacaaaaaac ggaaacagcg tgatgatgag aaacagcgcc agcccgcagt
     7501 aaaaaccgat acgactgagc gtgcgtttat ttgttgccat tgaggttcac ccgtttcctg
     7561 gtcagcagaa tgacagcgaa aataatgatg aagagcacca ccgagatcgc cgccgctttc
     7621 cctaagtcat taaaggcaaa ggcggttttg tagagataga caccaagaat atcgaccttg
     7681 gttgtcagca gataaacatc agcgaacatg tagaacatcc agatggtgcg cagggtcacc
     7741 accgtcgcca ggaccggcat aattgcgggc agcgtgacga tgcgaaaccg ctgccaggca
     7801 ttagcgccat ccatctctgc cgcttcgtac agcgatttgt cgatggtctg caaaatcgcc
     7861 agaaacgaga taaaggcata cgggaagtag cgccagatgg cgaacagcac caccagcaca
     7921 aagctactgc ccggattatc aaaccacagc ggtgcctgct catagagatg cagaagatcg
     7981 acgccgaggt agttcacaat gccgtagccg ttgttgaaca tgtatttcca ggcaaacacc
     8041 agcgaaatgg acggcgttac gtaggagagg atcaccagcg aacgcgcggt tttgcgcagg
     8101 cggaactcgc ggttgaaaaa catcgccacg gccagcccga gcacggtgct acccgccacc
     8161 accagcgccg tataccagac cgtcatccac agcgagtgcc agaagccggg atcggagaga
     8221 atgcgcacat agttactcac cccaacaaac gttgactcga tgttgggatt gagcggcaga
     8281 cgtaaaaaac tgatttcgat attcgacacc atcggccacg ccaccagacc gcccagcagt
     8341 aataagctgg gcgcgagaag cagcagcgca aacggcatat cggaacgacc tgaaaaaagc
     8401 ctgttcattg cggattcctg tcctgggttt agtgctgttc gatcagttca tccagctttt
     8461 tctggctcgc ttgcagcgtg ccagggagat cggctttacc cacggtgacg ttatgcacca
     8521 tggagctcac cacaccagaa cccgtcacat cgcccatgcg ggtaaagttt ttatccccta
     8581 ctgcgccaaa gacctgaata tttggcagtt caccgattag ctgattcggt agttcaccca
     8641 gcgccttaat aacgtcgttg tctttccagg tggcggtagt gacaaccgct ttattcaccg
     8701 gcagcgcagc acctggcgac atcatcaccc agtcggcaat gttgtccgcc tgctccataa
     8761 aggtgacaaa tttttctgct gcttcggtct tttgtccggc ggtaatggtc agcgaggcca
     8821 acatgccgta gaccgcagag tttttctcgg ttggcaccac gaaaccgacg tttttcgggt
     8881 cgccttcttt aatcacagcc ggaaggatat aggtggagta aatcgccatc ggcgcggtgc
     8941 cgttcataaa ggcgtctttc acttccatga tgtcgttaga acccggcatg gtgttggcag
     9001 caaggtcgcg gtaataggtc agcgcctgca tcatctctgg tgtatcaagg gtgattttgc
     9061 cttcggcgtt aaagacgttg gcctggttgg ataacgcaaa ctgggaaaag gattgttccg
     9121 tcagcacgct ttctgccgta ggcagcgcaa taccgtattt tttattcgcc gggtcattca
     9181 gtttctgtgc aacgtccagc agctgttgcc tgtttttcgg ctcctccagc cccgcttttg
     9241 ccagcacatc tttgcgatac cagatgccgc caatccaggc gctgacagga acaccggtcc
     9301 atgcgctacc atcttcggta cgcacaatac gcagtacgcc atcgtaaaac gcgccttcac
     9361 caacgttgct gatgaccgtg gcaaccgctt tgcgatcgat aagctgctct ttatccataa
     9421 ctttggcgta gtcatggctg gtttcgatca cttccggcag cgagccgcta cgtgaaaggg
     9481 tgatgacttt ggtgttataa gcgtcttctt ccacgggcac ttgtttaacg gtgatacctg
     9541 ggttttcttt ttcaaaacgg gcaatcaatt tgctgataac tgcctgacgc tcctgctcca
     9601 ccgaagaatg cataaattcg atggatacat gggttttatt ttcttcttta cagcctgaaa
     9661 tcagggcgca ggaaaccagt gctgataaca gcacgatttt tgatttaatc atgagatgtt
     9721 cctttttatt taatccacat aacctgccag gggcgcagcg ttatttcatt acccaatata
     9781 tcaacttcac taatcagatc ccgaccatgc agatcagtaa tatttacatg ctgaatattt
     9841 ttactgacat taaacaatcc agtcagacaa ttaccatcag cgttacttct ttgaatacgc
     9901 attacggatg aattaaccgt atcgatggta aaattattat ccggatgaaa ctcgttatgg
     9961 ctgcgacgaa gtgtaattaa acgcgacaac tcatgatata ccgcatgcct taatgtagct
    10021 tcatcgttca gttctcgtgt tatctcttca ctgtgatatt ttttacggtt aatcgcacgg
    10081 ttatatccga gtttttcgac acctgcgtaa tcattacgcg agccaagaat actttgaata
    10141 tatatcgcgg gtacaccggg gaaacttaac aaaatcgcat gggcaaggat aaacctggcg
    10201 caacgttctt catcgctact ctcgcggcgg cttaacgcat ccatataggt gacattcatc
    10261 tcatagggac tgcgcgtgcc gtcgggatta tttttccagt ttactaatgc accttcctgc
    10321 tgtaatgcct cgaccagcgc taatatttcg ctttctggaa gcaagccacg cagtgggttc
    10381 aacccgatgc catcgtgaga ggcgaggaag ttaaaccagg tcgttttact ggagggcaag
    10441 ctcagacttt gcgcccactg gcacagcgcc tcgacgttct gtttttgcac cgcatgtaac
    10501 accagcggcg gcagcgagaa ctggtacacc atatgcgctt cgtcatcacc gttgccaaag
    10561 taagcaatgt tgtctttgtg cggaacattg gtttcggtaa tgatcactgt acctggtgct
    10621 acgtcatcaa taatcgaccg taacagcttg ataatcagat gtgttttttc cagatggatg
    10681 cagcttgttc ccggctcttt ccacataaag ccaacggcat ccaggcggac atactcagca
    10741 cctttctcaa ggtaacagag taaaacatcc accatcgcca gcaacacttc agggctacgg
    10801 taattcaggt caatttgagt cactaaaggt ggtccataaa tggcgcgtgg aattatcatc
    10861 catttgaaac ggcgttaata gcggtaacgc acgcggacgg gtgacggcac tgagatcggt
    10921 tttcggatca accgcaataa agaaatcttc aaaacccgga agctgttgta aatagttttt
    10981 aaaccattca ctttttgcag acatatggtt gcagacaaaa tcaaacatta aatgactgca
    11041 ttcaccgagt tgctgaatat cctgccactc ccccgcttca ctggcgacct gatgataatc
    11101 aattaccgaa aatccatcat cagatgacca tgggtaaaaa ggtaataaat gaacatgtga
    11161 aaaaatgctt tgcagccatt gatggtagaa ctgattaaat atgggtagtg gttttaaatc
    11221 attactgtga aattgatcgg cataggtaat taacacgaca tcactttcat cccagtgttt
    11281 tttacgtcgc tgtgtaatta atcgtttcgc actctcaaga cgcgttacaa gtttctgtaa
    11341 atgagttgcg gtaaatgttc caccgtagat ttcgtccagg taatccgtaa ttttctgttt
    11401 catgttgcct tttcgtggta gcggtcccat gaagcgagag actactccgc agaaaataaa
    11461 ctgtcaattg agcacaaggg ttttagtcac gcaggaaatc attttttacc agggaaaaag
    11521 cgtatttgtg agctgacgca aataatctat agggatgtaa ataaggtgtg acagaaaaaa
    11581 aacggcgcat aagcgccgct catggtgaat tcttttaacc tttgaccttc ggcattgcga
    11641 tgtctttcag gccaccggcg ttctcaacgt gggtatatcc catctcgcta aggatctctt
    11701 ttgcctgccc ggactggcgt ccggcattgc aatacacttt cacggtgtcg tttttatccg
    11761 gaacggcggt gacaatgcgc tctttcactt ccttcagggg aatattgatg gccccctgaa
    11821 cgtgctcttg ctgatactgc tctggaacac gaacatcgat ccagtgttca gcggcgaaaa
    11881 cgggcagtga aaacaccagt gccagagcaa gtaagccttt ttttaaacat gtaagcgtcc
    11941 ttctatggat gaaaattgcc atccattata cgcctgagat ttaacaattt ttgcgaaact
    12001 cccctcacct tttataacgc tgtgccagtt tattagcagc ccgacttaac agcggttcca
    12061 gcgccacagc cagcaacatt ttcagcggtc ggcgagcaac tgattttatc gcccagcccg
    12121 ccacacccgc cgggccatag cgcagtgcgg taagaagtac cagcttgcct gctaatttga
    12181 aaccaggctt caccttttgc ccggcctgtt gccagcgagt attcataact ttcctcacag
    12241 ttgacggaaa cggctacgta acgtgaaagt atcggaagtg acataacgtt ccatctcgcg
    12301 taaacgcgtt tcacttgccg ccagttcacg gtcgacttca tccagcaatt cgctgctgga
    12361 aggtagctgc tcaccaaagg ccatgttgtc cggcattgga tcaagcgcaa atgacaaaat
    12421 gatgtaagca accagggtaa acagcgccag accgaagaaa atcgacagca ccaccaggat
    12481 acgcaccagt tttaccggta catcaaaata gttggcaatc ccggcgcaga cgccgcggac
    12541 catgccctgc tgtggaatac gccataattt tttattgaga ttaatgcccg ccattagcga
    12601 tccctccagt tcggatgttc tgcatcaaga atagattcca gcgcctgaat acgttcgcgc
    12661 atccgttttg cttcatcagc cagttgcgct aatcgctgct gctcactttg cgacaattca
    12721 ctgcgaccag aacgattgct gtaatgcagc cataaccaga tcggtaaaac aaacagcaca
    12781 aaaatggtta acggaatagc cagaaatagc gcgctcataa gtactcctta gacgatgagc
    12841 ggcggcgcgt tcggacgccg cctggatgtt attattgatt gtcttgcttc attttggctt
    12901 ttaattgtgc cagttgttcg ctgattgcat catcggcttt cagttcggca aactgatcgt
    12961 ccagcgattt ttgtttaccg aagctgtggc tttctgcttc cgcttccatc tggtcaatac
    13021 gacgttcgaa agattcgaaa cgagccattg ctttatccag tttgccactg tccagctgac
    13081 gacgcacatc gcgcgacgag tttgccgcct gatgacgtaa catcaatgcc tgctggcgag
    13141 cgcgtgtttc gctcaatttg ttttccagct cgccaatctc tttcttcatg cgtgccagcg
    13201 tatcgtccac cagcgtcact tcatgttcca gggacttaat cagatcggtc agtttctgtt
    13261 tttcaattaa cgctgcacgt gccagatcct ctctctcttt cagcagcgcc agttcggctt
    13321 tttcctgcca ttcaacttca cgcgccgacg cttgttcaat acggcgagtc agctgtttct
    13381 tttctgccaa cgcacgcgcc gaagtagaac gtacttcaac cagtgtatct tccatctcct
    13441 ggatcatcag acgcaccagt ttctgtggat cttccgcttt ctctaacaga gcgttgatgt
    13501 tggcattcac gatgtcggca aagcgagaaa aaatacccat aatgttgtcc tcttgatttc
    13561 tgcgttcagg attgtcctgc tgaactgtta atacaatttg cgtgccaatt ttttatcttt
    13621 ttgatttata aagatctgat tgaagaatca acagcaacat gccaggatga attagcgaat
    13681 tacactaaca agtggcgaat ttcatcatgg ccgaatacaa agataattta cttggtgagg
    13741 cgaacagctt tctcgaagtg ctggaacagg tttcgcatct cgcaccgctg gacaaaccgg
    13801 tgctcatcat cggcgaacgc ggcaccggta aagagttgat tgccagccgc ctgcattatc
    13861 tctcctcccg ttggcaaggg ccgtttattt cccttaactg cgcggcgtta aatgaaaatc
    13921 tgctggattc cgaactgttt ggtcacgaag cgggggcgtt taccggtgcg caaaaacgtc
    13981 atcctgggag atttgaacgt gccgacggcg gtacgctatt tcttgatgaa ctcgctacgg
    14041 caccgatgat ggtgcaggaa aaattattgc gtgtgattga gtacggtgaa ctggagcgcg
    14101 ttggcggtag ccagccgttg caggtgaatg tgcggttggt atgcgcgacg aatgccgatc
    14161 tcccggcgat ggtcaatgaa ggtacttttc gcgctgacct gctcgaccgg ctggctttcg
    14221 atgtagtgca actgccgccc ctgcgcgagc gcgaaagcga cattatgctg atggcagaac
    14281 actttgccat tcagatgtgt cgggaaatca agctgcctct gttcccgggg tttacggagc
    14341 gcgccagaga aacattgctg aattatcgtt ggccgggaaa tattcgtgaa ttgaaaaacg
    14401 tggtggaacg ttcagtgtat cgccacggca ccagcgatta tccgcttgat gacatcatta
    14461 ttgatccctt taaacggcgt ccacctgaag aagccatcgc cgtttcagaa aatacctcgc
    14521 ttccgacgct gccgctggat ttacgcgagt ttcagatgca gcaggaaaaa gagttgctgc
    14581 aactcagttt gcaacagggg aaatataacc agaagcgcgc ggcagaatta ctgggtttaa
    14641 cttatcatca gttccgcgcg ttgttgaaaa agcaccagat ttagcgaagt tgagcaatac
    14701 aacttgccgg atgcggcgtg aatgctttat tcggcataca taatcaatac gtcgtttgca
    14761 ggcctgataa gcgcagcgca tcagacatta ttgcgttggg ctattaatcg ctcagcgcat
    14821 cctgcaaaat tttcattgcc gcatcgaatt gcgcatccgg gatggtcagc ggatacagga
    14881 agcgaatcac gttgccgtat gcgccacagg tcagcaggag cagcccctgc gccagcgcgc
    14941 gttgctggat tttctgtgca atcgccgctg acggctcgcc cgtttgcgga tcgttaaact
    15001 ctgccgcaat catcgacccc aggccgcgta ccgcagcaat ggccggaacg ctttctttgg
    15061 catcaatcaa cgtgtttttg agacgctggc ccagttgatt cgcgcgttcg cagagtgatt
    15121 ctttgtcgat aatattgagc accgcgtgcg cggcagccac cgccagcggg ttcccggcgt
    15181 aggtgccacc caacccgccc ggcgcgggcg cgtccataat attcgcgtta ccgaccacgc
    15241 ccgaaagcgg catcccgccc gcgaggcttt tcgccatcgt cattaagtcc ggcttatcgg
    15301 cgtaatgatc catggcaaac agtttaccgg tacgcgcaaa gccgctttgc acttcatcgg
    15361 caatcatcac aataccgtgc tcgtcgcaca ggcggcgaat ggcggcaacc agctcttttg
    15421 gcgcaacgtt gaaaccgccc tcgccctgca ccggttcgaa aataatggcc gcgacctgct
    15481 tcgcttcgat gtctgattta aacaagcgtt cgatggcgtc gagggagtcc tgtgttgaaa
    15541 tgccgtgtaa atctgacgga taaggtacgt gatacaccga accagggaac gggccgaagc
    15601 cgattttgta cggcgcaact tttccggtca gcgccatggt catatatgta cgaccgtgga
    15661 agccaccgct aaacgcaatc acgccaggtc gtccggtatg ggcgcgagca attttcaccg
    15721 cgttttccac cgcttccgcg ccggtagtga agaatgcggt tttagcctgc ccgctcactg
    15781 gggcaagggc gttaattttc tccgccagcg tgacgtagct ttcgtacggc acaatctgat
    15841 acgccgtgtg ggtaaactgt tgcaattgct gctccaccgc cgcgaccaga tcagggtggc
    15901 gatgcccggt attcagcacc gcaatgcctg cggcgaaatc gatgtattcg ttgccctcta
    15961 cgtccttcag cgtagcgttt tctgccgact gggcgaagaa gttacacatc acgccaaccc
    16021 cgcgtggggt agcagaaaga cgacgctgat ggaattcatt gttgctcatg tttcacacct
    16081 tttgactctt cactgttaat cggaaattaa acgcccagac gatcgcgcag gctgtaataa
    16141 gccgcgccca tcgcggtaaa tggcacgcgc agcgtgcgcc cgccggggaa cgggtaatgc
    16201 ggcagattgg cgaaggcatc gaaacgctcg gcgtcaccgc gcagcagttc agcaatcaaa
    16261 cgcccggcaa gatgggtaca ggtgacgccg tggccgctgt aaccctgcat gtaatagatg
    16321 ttggtatcaa ggcggccgaa ctgcggcatg cgcgacaagg tcagcaggaa gttgcccgtc
    16381 cagcggtaat caattttcac gcccttcaac tgagggaagg tttttagtag tttcggtacc
    16441 acaagacgct caacgtcatc cgggttgcgt gcaccataca ctacaccccc gccgtacagc
    16501 aggcggttgt cggcggtaag acggtagtaa tcaagcagat agttacagtc ttccacacag
    16561 tagtttttcg ggatcagcga acgggctaaa tcttccgaca gccgttcggt ggtgatcacc
    16621 tgcgtgccgc acggcatgct gcgtttcgct agttccggct ctactttatc gcccagatac
    16681 gcattcccgg cgacaatcac atacttcgcc gtcacctgac cattggcagt ccgaaccacg
    16741 gctggcgtgg tgtgctggat ctgcgtcacg gcggaaagct catacacgcg cccgccgttc
    16801 aggcggatgg catcggcttc accaatggcg aggtttagcg ggtgaatatg cccaccgctg
    16861 tgatccagca gcgcaccagt gtagcgatcg ctggcgacct cgcggcgaat agcgttggcg
    16921 tccagcagct ccagctgttt attgccgtag cgttcccagt tctctttctg ctcttcgagt
    16981 gtggcgagtt gtttatcatt catcgccaca aacagaccgc cggggcggta gtcgcagtca
    17041 atctgataac gtttgatacg ttcgcggatg atctcgccac cctcgaacat catgctgccg
    17101 agcatacggg cggtgtccat gccgtagctt ttttcgatca cgtcgatgtc gcggctgtag
    17161 gagttcacaa gctgcccgcc attgcgcccg cttgcgccaa agccgatgcg tgaggcttcg
    17221 agaaccacta cgtcaaagcc cgcttccgcc agatgcagcg cggaggagag cccggtatag
    17281 ccgccgccaa ccacgcaaac gtcgcaggtg attgactcat tcagtgtgtc gaatggtgca
    17341 tatttattcg cactggcggc gtagtaactg ctggtatgtt cggtcatttc aggcctccag
    17401 gcttatccag atggttttca gttcagtgaa tttttcaagg gcatgcaggg atttgtcgcg
    17461 accgttgccg ctctgcttat agccgccaaa cggcacggtc atatcgccgt cgttgtagtt
    17521 attgacgaag acggaaccgg ctttcaggcg tcggctcatg cggtgcgcgc gggagaggtc
    17581 gcgcgtccat accgccgcgc caaggccgta ctggctgtcg ttggcaagct gcagcgcctg
    17641 atcttctgat gtgaaacggg tgaccaccag caccggaccg aaaatctctt cgcggcttaa
    17701 ggacgcattc gggtccacat ccacgaagat ggtcgggccg atggccgctg ccagcccggc
    17761 gttgcgacca tccagcagca gttgcccctt gccttcgcct tcacgaataa aggtgtgtac
    17821 cgagtcagcg tgagcgctgt cgattaaggt gcccatggtg gttgcgggat caagtggatg
    17881 gcccggctgc cagttttgcg cctgctgttt taacagggct aagaattcat cggcgatgct
    17941 ctcttccagt aacaaacgcg ttccggcgat gcacacctgg ccctggttgt agaagatgcc
    18001 tgcggcggtg gcgcttgccg ctttttgcag atccggacaa tcggcgaaaa cgatgttggc
    18061 gcttttgccg cccgcttcca gccagacacg tttcatgttg ctatcacccg cgtctttcag
    18121 cagttgttta cccgtacgcg tggagccggt aaaggcgatg gcatcgatat cgttatgacg
    18181 cgaaagcgcc tgccccgctt catgaccaaa acccgtcacc acgttcaaca caccatccgg
    18241 caagcctgct tctttcgcca gccccgcgag acgaatcgca ctgagcggtg atttttcaga
    18301 cggttttaga atcacgctgt ttcccgccgc cagcgccggg ccgagtttcc agcaagtcag
    18361 caacagcggg aagttccacg gcacgatggc ggcaatcacg ccgaccggtt cacgcacgat
    18421 catcgccagc tcatggctac tggtggtcgc cacttcgcca tacactttgt cgatcgcttc
    18481 ggcgtaccag cgaatggcgc gcgccgcgcc gggaatatca tcacgcagac tgtgacgaat
    18541 cggtttgccg gtgtcgagag tttccagcag tgccagctct tcggcgtggg cttccattaa
    18601 atcggcgagt ttattcagta ccgctttacg tttagccgga gaagagagtg accagtcgcc
    18661 gcgttcaaat acgccgcgtg ctgcgctcac cgcacggtcg atatcgacgc tcttgccgcg
    18721 ggcaattttc gccagcggtg cctgggtgac cggatcaacg gtttcaaagg tttcattttc
    18781 cgccgcagca gtatattcac cgttaataaa taagcggttt tcaatggcga gacttaacgc
    18841 tttatcctgc cagtaagcca gatgatgaaa attcattatg actcctgttt cacgtctatc
    18901 agatatatgc agtttgtgaa cgtcagggcg cagagatccc cgggctgcat gcccgtttat
    18961 ttctggcgac attctttatg gattgccggg cgcattgcgc ttacccggct tacagaacaa
    19021 aaagattaaa acgtggtggg cgtatgggcg ctgataattc ggcaaatacc tgccgacgta
    19081 ttactgaaac tgtgcgggat gccggtatta atggcataac tttgccccgc gacgaggtgg
    19141 taatcctgac cattaatcgt cagaacaatt tcaccttcca gtactgtgcc tatttcctca
    19201 ccctgatgct taattctttc cccagtggtt gtgcccggct ggtacgtttc aaagatcatc
    19261 gccagcgtgc gattcgggtt accgttatga accagcttca ttgacacacc ctgactgccc
    19321 atctcaatta agtcgtcctg attaatgacg acctgcggct catcaggttt ttccggctcg
    19381 gaaaagaatt ccgagagtga cagaccatac accttcagca gcttttgcag cgtactgatg
    19441 gcagggctga ctttatcttg ttctatcgta ctgatagcac tgtgagtcag cccggagagt
    19501 tcggcggcac gacgttgtga aagcccctgc tgctggcgga tttccgacaa gcgttttcct
    19561 ggcgccagtc cctcatcact catatttgca tttccttaaa ctgtagtggt cagagtcgct
    19621 gtttttcagc gatatggtgc tgccaagcgg tgataaagcc ctcgaacaat atacgcgaaa
    19681 gcgcgtactc gctactgttc cattccgggt gccactgtac gcccagcgca aaaggatgat
    19741 tgatgacgct aaccgcctca actaagccat ccggcgagcg agcttcaaca cgcaaccgtg
    19801 ggctaacgac cttcgcccct tgtccatgta gagagtttac ccaaaagttg ctacattcag
    19861 gtaacaacgc agacagtaat cccccctctt caacctgaac ttcatgcgac ggtgcatatt
    19921 gctgttccac cggcagttcg ggatcttccc gatgttccag caattcaggc tgttcgcaca
    19981 gcttgcgatg caacgaacca ccggttgcca ccaccagttc ttgtaaaccc cggcagatgg
    20041 cgaaaatggg gatgcgcctt tcgagtgcgg cattaattat cgccatgctc agaagatcac
    20101 gcccgggatc ggcgtcaggc tcatcgccgt tttcaccata taggtgcggc tgcacattgc
    20161 tgggactact aggaagataa atgccatcga gtttcggcaa aagttgttca agtaacgacg
    20221 gttccgccag cgcatgtggt agcgcaattg gcaagccgcc tgcgtggatg atggcattca
    20281 ggtacttttc ttgcagagtc tgggtcgcat gacccttaag cctgttcctg cacattacga
    20341 caccgataac cggattgttc attatatttt ccatgctcaa tctcacaaag tggactaaat
    20401 tatcgccatt actgcgcttt aaatgacctt tatgttcaat attttttcaa tctagcagtg
    20461 ggttttcatt tttgcaaact caatttaaca tttgacaaac atttagtttg catacagatt
    20521 cgaatgatgg tcattatatt ttacgctttg ataacgagcg gaaaacaaac caaaggcgaa
    20581 gaatcatgga aaccaatatc gttgaagtag agaactttgt tcagcagtca gaagagaggc
    20641 ggggtagcgc ctttacgcag gaagtgaaac gctacctgga gcgctacccg aatacgcaat
    20701 atgttgatgt tttacttact gatttgaatg gttgcttccg tggaaaacgt atccctgttt
    20761 caagcctgaa gaagctcgag aaagggtatt atttcccggc ctcggtattt gcaatggata
    20821 ttctgggcaa cgtagtagaa gaagcaggtc tgggtcagga aatgggcgag ccggatcgta
    20881 cctgtgttcc tgttctcggt tccttaactc cttctgccgc cgatccagag tttatcggtc
    20941 agatgctcct gaccatggtc gatgaagatg gcgctccctt tgacgttgag ccgcggaacg
    21001 ttcttaaccg cctgtggcag cagctgcgcc agcgcggatt gttcccggtt gtagcggtag
    21061 agctggagtt ctatttactg gatcgccggc gcgacgctga aggggatctg caaccgccct
    21121 gcgcgcctgg caccgatgac cgcaatacac aaagccaggt ttactccgtt gataacctca
    21181 accacttcgc cgacgtgctc aatgatattg atgaactggc gcagttgcag ctgattccgg
    21241 cagatggcgc ggtcgctgag gcctcgccgg gtcagtttga aatcaacctt taccatactg
    21301 ataacgtgct ggaagcctgc gatgatgcgc tggcactaaa acgcctggtg cgtctgatgg
    21361 cagaaaagca taagatgcac gccactttta tggcgaagcc gtatgaagag cacgcgggca
    21421 gcgggatgca tatccatatc agtatgcaaa ataatcgtgg cgagaatgtg ctttctgaca
    21481 cagaaggcga agattcgccg ctgctgaaaa agatgctcgc cgggatgatt gacctgatgc
    21541 cgtcgtcgat ggcgttgctg gcaccaaacg tgaactcgta tcgccgcttc cagccgggaa
    21601 tgtatgtgcc gacgcaggcg tcgtggggcc ataacaaccg caccgtcgcc ctgcgtattc
    21661 cgtgcggcga ccgtcataat caccgcgtgg aatatcacgt ggcgggtgcc gatgccaacc
    21721 catatctggt gatggcagcg atttttgccg gtattttgca tggccttgat aacgagctgc
    21781 cgttgcagga agaagtcgaa ggcaacgggc tggaacagga aggcttaccc ttcccgattc
    21841 gccagagcga tgccctgggt gagtttatcg agaatgatca cctgcgccgc tatttaggcg
    21901 aacggttttg ccatgtgtat cacgcctgta aaaatgatga gctgttgcag tttgagcgcc
    21961 tcatcacaga aacagaaatt gagtggatgt tgaaaaacgc gtaattcccc ttgcggggtt
    22021 gtttcatcgg cctgagcgga gccaggcctg acatttgctg gtaatccaga catttcccgg
    22081 atgtcgcgtt atggagcgca ggcggcaacg ggcttccgtt aacgattaag actgtgacga
    22141 ggaaatgaga tgatgccgat gattaaatcc ccgcatggcg agggaggctg cgtatgcgcc
    22201 cctcctgcta ctgactggac tccgccccct ttgcttccct tgctaaacag gtttgatttc
    22261 aggtcgacac gaccgcaaac cttattacgc aggggaggca gcaattatgg ctattaattc
    22321 accactgaat attgctgcgc aacccggcaa aacccgtctg cgaaaatcac tgaaattgtg
    22381 gcaggtggta atgatgggtc tggcctatct cacgccgatg accgtatttg atacttttgg
    22441 tattgtctcc ggcattagcg acggtcacgt tccggcgtcc tatttgctgg cgctggcggg
    22501 cgtgctgttt accgctatca gctacggcaa actggttcgc cagtttccgg aggccggttc
    22561 ggcctatacc tacgcgcaaa agtcgattaa cccgcacgtc ggatttatgg tcggctggtc
    22621 atcactgctg gattatctct ttttgccaat gatcaacgtt ttgttggcga aaatctatct
    22681 ctccgccctc ttcccggaag tgccgccgtg ggtgtgggtg gtaaccttcg tcgccatttt
    22741 aaccgccgcg aatctgaaga gcgtcaacct ggtcgctaac ttcaataccc tgtttgtact
    22801 ggtgcaaatc tccatcatgg tggtgtttat cttcctggtg gttcagggac tgcataaagg
    22861 agaaggcgtt ggcaccgtct ggtcacttca gccgtttatc agcgagaacg cgcacctgat
    22921 cccgattatt accggtgcga cgattgtctg tttctcgttc ctcggtttcg atgcggtgac
    22981 cacgctttcg gaagagacgc cggacgccgc acgcgtgatc ccgaaagcca tcttcctgac
    23041 ggcggtatat ggcggcgtta tctttatcgc ggcgtcgttc tttatgcagc tgttctttcc
    23101 cgatatcagc cgctttaaag acccggacgc cgcactgcct gaaattgcgc tgtacgtcgg
    23161 cggcaagctc tttcagtcga ttttcctctg caccacgttt gtgaacacat tagcgtctgg
    23221 cctggcttca catgccagcg tgtcgcgtct gctgtatgtg atggggcgtg acaatgtgtt
    23281 tccggagcgc gtgtttggct atgtgcaccc aaaatggcgg actccggcac tcaacgtcat
    23341 tatggtcggg attgtcgcgc tgtcggcgct gttctttgat ttagtcaccg cgacagcact
    23401 gattaacttc ggtgctctgg tggcgtttac cttcgtgaat ctgtcggtgt ttaatcattt
    23461 ctggcggcgt aaaggaatga ataaaagctg gaaggatcac ttccactatt tgctgatgcc
    23521 gctggttggc gcgctgacgg tgggtgtgct gtgggttaac ctagagtcaa cgtcactgac
    23581 actcggtctg gtatgggctt cgttaggcgg cgcatatttg tggtatctga tccgccgcta
    23641 tcgcaaagtg ccgctgtacg aaggtgacag aacgccggtg agcgaaacgt aatagccgga
    23701 tgcgcggcgc attatcctgc ctgagggtaa cgcgccgcgc cattaactat aaaaagatgt
    23761 atgacccgga tcacgagtgc tatcatccgc ccttatgctt agcgcattaa ccccaggggc
    23821 aaataatgaa ccacgatatc ccgctaaaat attttgatat tgccgatgag tacgcgaccg
    23881 aatgcgcaga gcctgtcgca gaggctgaac gcacaccgct ggcccactac ttccagttac
    23941 tgctcacccg tttgatgaac aatgaagaga tcagcgaaga agcccaacat gaaatggctg
    24001 ctgaagcggg gattaatccc gtacgcattg ataagatcgc agagtttttg aatcaatggg
    24061 gtaatgagta attatttaat tgcagacgat cggtcagtat cgttgtccgg ataaagcgtt
    24121 cacgccgcat ccggctaaca atgacacgca ctgcagcgtg ctttaatggg tttctcacga
    24181 tcgtaccgct ctgctcctcg ctaagttgtt ctgtgagtta tagttataga gaaatcacgg
    24241 gtacgaatgt tgaacagatg gcgacgccac ctgttcaacg accaaccaca cggccctcat
    24301 tacgacccct gaacacaaac ggtaatggca aatgtcatta aatattagta agatagcctc
    24361 tttacataaa atcccttaat attatgcgcc aggtattatc gtctcttttg gtgattgctg
    24421 gacttgtgag tggtcaggca atcgccgcgc ctgaatcccc cccgcatgct gatatccgcg
    24481 acagcggttt tgtctattgc gtcagcgggc aagtcaacac ctttaaccca tccaaagcga
    24541 gcagtgggtt aattgtcgat acccttgccg cccagtttta tgatcgactg ctggatgtcg
    24601 atccctatac ctatcgcctg atgccggaac ttgccgaaag ctgggaagta ctcgacaacg
    24661 gcgcgaccta tcgcttccac ctgcgtcgcg atgttccgtt tcaaaaaacc gactggttta
    24721 ctcccactcg taaaatgaat gccgacgatg tggtgtttac tttccagcga atttttgacc
    24781 gcaacaaccc gtggcataac gtcaacggca gcaacttccc ctacttcgac agcctgcaat
    24841 ttgccgataa cgtgaaaagc gtccgcaaac tggataatca taccgttgag ttccgactcg
    24901 cccagccgga tgcttctttt ttgtggcacc tcgcaaccca ttatgcttcg gtcatgtcgg
    24961 cagaatatgc ccggaagtta gagaaagaag atcgccagga gcaactcgac cgtcaaccgg
    25021 tcggcaccgg tccgtatcag ttgtcggaat accgcgccgg gcaatttatt cgcctacaac
    25081 gtcatgatga cttctggcgc ggtaaaccgt taatgccgca ggtagtggtg gatttaggct
    25141 ccggcggcac cggacgtctg tcgaaactcc tgaccgggga atgcgacgtt ctggcctggc
    25201 ctgctgccag ccagctatcc attttgcgtg acgacccgcg cttgcgttta acgctgcgtc
    25261 ctgggatgaa cgtcgcctat ctggcattta acaccgccaa accgccgcta aataatcccg
    25321 ctgtccgcca tgcactggca ctggcgatta ataaccagcg cctgatgcaa tccatctatt
    25381 atggtacggc tgaaacggcg gcctctattt taccgcgcgc ctcgtgggcc tatgacaacg
    25441 aggctaaaat tactgaatac aatccggcga aatcgcgcga acagttgaag gcgttggggc
    25501 tggaaaactt aacgctgaaa ctgtgggtgc ccacacgttc gcaggcgtgg aaccccagtc
    25561 cactgaaaac tgccgaactg attcaggcgg atatggcgca ggttggcgta aaagtggtga
    25621 ttgtgccggt agaaggtcgc tttcaggagg cgcggttgat ggatatgagc catgatctga
    25681 cgttatccgg ttgggcgacg gacagtaacg acccggacag tttcttccgt ccgttactga
    25741 gctgcgcggc aattcattca cagaccaacc tcgcccactg gtgcgatccg aaattcgaca
    25801 gcgtgttgcg taaggcgctc tcctcgcagc agctggcagc gcgtattgaa gcctatgacg
    25861 aagcgcagag tattctggcg caggaattac ctattctgcc gctggcgtca tcattgcgtt
    25921 tgcaggccta tcggtacgat atcaaaggtc tggtacttag cccgtttggt aacgcctcct
    25981 ttgctggggt gtatcgcgag aaacaggatg aggtgaaaaa accatgatta tcttcacctt
    26041 acgccgcatt ttgttattga ttgtcacctt gttcctgctg accttcgttg gcttcagctt
    26101 gagctatttc actccacacg cgccgttaca aggcgcgtca ctgtggaatg cctgggtgtt
    26161 ctggtttaat ggcctgatcc actgggattt tggtgtttcc agcattaacg gtcaaccaat
    26221 tgccgaacag ttaaaagagg tcttcccggc gacgatagaa ctgtgcatcc tcgccttcgg
    26281 ttttgcgctg attgttggca tccccgtggg gatgattgcc gggattacgc gccataagtg
    26341 gcaggataac ttgatcaacg ccatcgcctt actgggcttt tcaatcccgg tgttctggct
    26401 ggcgcttctg ttgacactgt tttgttcact cacgctaggt tggttgcccg tttcagggcg
    26461 tttcgatctg ctctacgaag tgaaaccgat taccggtttt gcgttgattg atgcctggct
    26521 ttcggactca ccatggcggg atgaaatgat catgagcgca atccgccata tgatattgcc
    26581 cgtgatcacc ctgtcggttg cgccaacaac tgaagtgatc cgcctgatgc gtatcagcac
    26641 tattgaagtg tatgaccaga actatgtaaa agcggcggcg acccgcggtt tgtcgcgctt
    26701 tactattttg cgtcgccacg ttctgcataa cgcgttaccg ccggttattc ctcgcctggg
    26761 cttacagttt tccaccatgc tgacgctggc gatgatcacc gaaatggttt ttagctggcc
    26821 gggtttaggg cgctggttaa ttaacgccat ccgccagcag gattacgcag ccatttccgc
    26881 cggagtgatg gtgtgtggct cactggtcat tattgttaac gtgatttctg atattttggg
    26941 tgccatggct aaccctctga aacataagga atggtatgcc ttacgatagc gtatacagcg
    27001 aaaagcgccc gccgggcacg ctgcgtaccg cctggcgcaa attttatagt gacgcctctg
    27061 cgatggtcgg cctgtacggc tgcgcgggac tggctgtact gtgtattttt ggcggctggt
    27121 ttgcgcctta cggcatcgac cagcaatttc tcggttatca attattgccg ccatcatggt
    27181 cgcgctatgg cgaagtttct ttcttcctgg ggactgacga cctcggacgt gatgttttaa
    27241 gccgtttact gagcggagct gcgccaaccg tgggtggcgc atttgtggtg acgcttgccg
    27301 cgacgatctg tggcctggta ctgggcacct tcgccggggc gacacacggc ctccgctcag
    27361 cggtgcttaa ccatattctg gataccttac tggcgatccc ttcgctgctg ttggcaatta
    27421 tcgttgtagc gtttgccgga ccgagtttgt cccacgctat gtttgctgtc tggctggcat
    27481 tgctgccgcg tatggtacgt tcgatttaca gcatggtgca tgacgaactg gaaaaagagt
    27541 acgttatcgc cgcccgtctg gatggcgcat caacgctgaa tattctctgg tttgctgtga
    27601 tgccaaacat caccgccggg ctggtcaccg agatcacccg cgcactgtcg atggcaattc
    27661 tcgatatcgc cgcgctgggc tttctcgatc tcggcgcaca actcccctcg cctgaatggg
    27721 gagcgatgct cggtgatgcg ctggaactga tttatgtcgc cccgtggact gtcatgctgc
    27781 caggtgcggc aattatgatt agcgtgttgt tggttaacct ccttggcgat ggcgttcgtc
    27841 gtgcgattat tgcgggggtg gaataatgcc attactcgat attcgtaacc tgaccattga
    27901 atttaaaacc ggtgatgagt gggttaaagc cgtcgaccgg gtaagtatga ccttaaccga
    27961 aggtgaaatc cgcggtcttg ttggtgaatc cggttccggc aagagtttga ttgcgaaagc
    28021 gatttgtggg gtgaataaag ataactggcg tgttactgct gaccgtatgc gttttgatga
    28081 tatcgatttg ctgcgtctct ccgcacgcga acggcgcaaa ctggttggcc ataacgtgtc
    28141 gatgattttc caggaaccgc agtcgtgtct tgacccttca gaacgtgtgg gtcgccagtt
    28201 gatgcaaaac atcccagcct ggacctataa aggccgttgg tggcagcgtt ttggctggcg
    28261 caaacgccgt gccattgaac tgctgcaccg cgtggggatt aaagatcaca aagatgcgat
    28321 gcgcagtttt ccctatgagt tgaccgaagg tgaatgtcag aaagtgatga tagccattgc
    28381 actggcgaat caaccgcgtc tgctgattgc tgacgaaccg acaaactcaa tggagccaac
    28441 aacccaggcg caaatctttc gcctgctgac gcgtctcaac caaaacagca ataccactat
    28501 tttgcttatc agccatgact tacaaatgct tagccaatgg gcggataaaa ttaacgtgct
    28561 ttactgcggt caaacagtgg aaaccgcgcc gagtaaggag ttggtgacga tgccacatca
    28621 tccttatacc caggcgctga tccgcgcgat accagacttc ggcagcgcga tgccgcataa
    28681 aagtcgcctc aatactctac ccggcgctat cccattgctg gaacagttac cgattggttg
    28741 tcgtctgggg ccacgttgcc cgtatgcaca acgagaatgc attgtgacgc cacgtttgac
    28801 gggggcgaaa aatcatctct atgcctgtca tttcccgctg aacatggaga aagagtgaga
    28861 tgatcgaaac gctgcttgaa gtgcgtaatt taagtaaaac gttccgctac cggaccggtt
    28921 ggtttcgtcg tcagaccgta gaagcggtaa aacccttgag ctttacgcta cgtgaaggcc
    28981 agacactggc gattattggc gagaatggtt cgggtaaatc cacactggca aaaatgctgg
    29041 cgggaatgat agaacctacc agcggcgagt tattgattga cgatcatccg ctgcattttg
    29101 gcgattattc cttccgtagt cagcgtattc gcatgatttt tcaggatcct tcgacctcgt
    29161 tgaatccccg tcaacgtatc tcgcaaattc tggattttcc actgcgcctg aacaccgacc
    29221 tggaaccgga acagcgtcgc aaacagatta ttgaaacgat gcgtatggtt gggctgctac
    29281 cggatcacgt cagttactat ccgcatatgt tggcacccgg acaaaaacag cgtctgggtc
    29341 tggcgcgcgc gctgatattg cgcccaaaag tcattattgc cgatgaagcc ctcgcttcac
    29401 tggatatgtc gatgcgttcg cagttgatta atctgatgct ggagttacag gaaaaacagg
    29461 gcatttcgta tatttatgtt acccagcata tcggaatgat gaagcacatt agcgaccagg
    29521 tgctggtgat gcatcagggc gaggttgtcg agcgaggcag caccgcggat gtgctggcat
    29581 cgccgctgca tgaactcacc aaacggctga ttgccgggca ctttggcgaa gcattgacag
    29641 cggatgcgtg gcgtaaagat cgctaatttt ctgaatcctc cttccagatt attcacctca
    29701 ttctgacaag agattaaatc ttgtcagaat gaggtgatgg ataaaataaa atcaaatgcg
    29761 cgtgatttaa gacgcaatct cactttgcag gaacgaaagc tctggcgata tcttcgcagc
    29821 cgacgttttg gtgatttcaa atttcgccgt caacatccgg tggggagcta cattctcgat
    29881 tttgcttgct gctcggcgcg cgtagtcgtt gagctggatg gtgggcagca tgatttggca
    29941 gttgcctatg ataccaggcg cactagctgg cttgagtcgc agggctggac cgtgctgcgt
    30001 ttctggaata acgagattga ttgtaatgag gaggcggtgc tggagattat tctgcaggaa
    30061 ctgaaccgcc ggtcaccctc accctgaaag ggcgaggggg ccgaccgagc cgaatagctg
    30121 ttgtggtgaa aacatggaga cagtgctgga gaatattcgg caaggtctga accatcccag
    30181 ccatcgccat aaaagggtta ggggctgtat gagcctgttt gttgctgggg taacaatatt
    30241 tgcacaatat ggtcccctcg cccctctggg gagagggtta gggtaagggg aaaagcgccc
    30301 cccctgccgc agcctgctcc ggtcggacct ggcaactata gctactcaca gccaggttga
    30361 ttataataac cgtttatctg ttcgtactgt ttactaaaac gacgaatcgc ctgattttca
    30421 ggcacaacaa gcatcaacaa taaggattaa agctatgggt tttctttccg gtaagcgcat
    30481 tctggtaacc ggtgttgcca gcaaactgtc catcgcctac ggtatcgctc aggcgatgca
    30541 ccgcgaagga gctgaactgg cattcaccta ccagaacgac aaactgaaag gccgcgtaga
    30601 agaatttgcc gctcaattgg gttctgacat cgttctgcag tgcgatgttg cagaagatgc
    30661 cagcatcgac accatgttcg ctgaactggg gaaagtttgg ccgaaatttg acggtttcgt
    30721 acactctatt ggttttgcac ctggcgatca gctggatggt gactatgtta acgccgttac
    30781 ccgtgaaggc ttcaaaattg cccacgacat cagctcctac agcttcgttg caatggcaaa
    30841 agcttgccgc tccatgctga atccgggttc tgccctgctg accctttcct accttggcgc
    30901 tgagcgcgct atcccgaact acaacgttat gggtctggca aaagcgtctc tggaagcgaa
    30961 cgtgcgctat atggcgaacg cgatgggtcc ggaaggtgtg cgtgttaacg ccatctctgc
    31021 tggtccgatc cgtactctgg cggcctccgg tatcaaagac ttccgcaaaa tgctggctca
    31081 ttgcgaagcc gttaccccga ttcgccgtac cgttactatt gaagatgtgg gtaactctgc
    31141 ggcattcctg tgctccgatc tctctgccgg tatctccggt gaagtagtcc acgttgacgg
    31201 cggtttcagc atcgctgcaa tgaacgaact cgaactgaaa taatcgttct gttggtaaag
    31261 atgggcggcg ttctgccgcc cgttatctct gttatacctt tctgatattt gttatcgccg
    31321 atccgtcttt ctccccttcc cgccttgcgt caggataacg atttccttta cgaccaagga
    31381 gcgcccatgg aacaacgcca catcaccggc aaaagccact ggtatcatga aacgcaatcc
    31441 agtactacgg agtatgacgt tctgcctctg gtcccggaag ccgcaaaggt cagcgatccc
    31501 tttctgctcg acgtgatcct tgatgaagag acgctggccc ccttcctttc atggctggtc
    31561 cctgcgcgcg ttcttgcagt ggagttgttc cctgaccagc ttaccgtgac ccgttcacaa
    31621 accttcaccg cttatgaacg cttgtcgacg gccctgacgg ttgctcaggt ttgcggcgtc
    31681 cagcggttat gtaactacta ttcggcgcga cttacgccgc tccccggacc tgattcctcc
    31741 agggaaagta atcatcggtt ggcacaaatc acgcaatatg cccgccaact ggctagctcg
    31801 ccttctatta tcgacaaccg atcgcgccag catctgaatg acgtcggtct tactgtctgg
    31861 gactgtgtga tcattaacca aatcattggt tttattggct ttcaggcgcg gacaattgcg
    31921 acatttcagg cttatctcgg gcatccggta cgctggttac ccgggctggc gatacaaaac
    31981 tacgccgacg cgtcactgtt tgctgatgaa tcaatacgct ggcgaagcag ctatgaggtg
    32041 gaaaaactac ctgaagagca cacaaaaagt tcaactgcag aactttgcca actggccgaa
    32101 acactctctc tccaccctat ttcactttcc cttctcgaaa agttgttaaa cagcacacgg
    32161 gtcaatacac agccggataa tcagcttgcg gcgttgttat gcgcgcgtat aaatggcagt
    32221 cctgcttgtt tttccacctg tatggattca tcaaatgaat ataaaaaaat cagcaccctt
    32281 ctgcgcaagg gcgaaaatga aattaaccga tgggctgacc gtcattctgt tgagcgcgct
    32341 accgttcagg cgatacaatg gctgacccga gcacccgatc gctttagcgc cgcccagttc
    32401 agccctttac tcgaacacga aaaatcatca acgcagatta ttaatctgct ggtatggagc
    32461 gggctgtgtg gctggataaa tcgcttaaaa atcgcgttgg gtgagacata ttaaccttgc
    32521 cgcgtcagac agattcgcgt aaaactgtca gccgctctaa tggccaccaa aatagacaat
    32581 tatgtttcag gacaacccgc tgctagcgca gcttaaacag caactgcatt cccagacgcc
    32641 acgcgctgaa ggggtggtaa aagccacaga aaaaggcttt ggcttcctgg aagtcgacgc
    32701 gcaaaaaagt tatttcattc cgccgccgca gatgaaaaaa gtcatgcatg gcgaccgaat
    32761 tatcgcggtg atccacagtg aaaaagaacg tgaatccgca gagccagaag aactggttga
    32821 accgttcctg actcgtttcg tgggtaaagt tcagggcaaa aatgaccgtc tgaccatcgt
    32881 tcctgatcat ccacttttaa aagacgccat tccttgccgc gcagcccgtg gcctgaacca
    32941 cgagtttaaa gaaggcgact gggcggttgc cgaaatgcgc cgtcatccgc tgaaaggcga
    33001 tcgttctttc tatgctgaac tgacacaata catcactttt ggtgacgacc actttgtacc
    33061 gtggtgggtt acccttgcgc gccataatct ggaaaaagaa gcaccagacg gcgtcgctac
    33121 cgaaatgctc gatgaaggtc tggttcgtga agatctgacc tcgctggatt ttgtcaccat
    33181 cgacagtgcc agcactgaag atatggatga cgcccttttc gctaaggcgc tgccggatga
    33241 caaacttcag ctgattgtgg cgattgccga tccaaccgcg tggattgctg aaggcagcaa
    33301 gctggacaaa gccgcgaaaa ttcgcgcatt caccaactat ctgcctggct tcaacatccc
    33361 tatgctgcct cgcgagcttt ctgacgatct ctgctcactg cgcgccaatg aagtccgccc
    33421 ggtactggca tgccgcatga cgctctccgc tgatggcacc attgaagata atatcgaatt
    33481 ctttgccgcc accatcgaat ccaaagcgaa gctggtgtat gaccaggttt ctgactggct
    33541 ggaaaatacc ggtgactgga agcctgaaag tgaagcaatt gccgaacaag tccgtttgct
    33601 agcgcaaatt tgccaacgcc gcggcgagtg gcgtcataac cacgcactgg tgtttaaaga
    33661 tcgcccggat taccgcttta ttctcggtga aaaaggtgaa gtgctggata tcgtcgccga
    33721 gcctcgtcgc attgccaacc gtatcgtcga agaagcgatg attgccgcta acatttgtgc
    33781 ggcccgcgta ctgcgcgata agctcggttt tggcatctat aacgtgcata tgggctttga
    33841 tccggcgaat gccgacgcgc tggcagcgtt gctgaaaacg cacggtctgc atgtcgatgc
    33901 cgaagaagtg ctcacgctgg acggtttctg caaactgcgt cgtgaactgg atgcgcaacc
    33961 aactggtttc ctcgacagcc gcattcgtcg cttccagtca tttgctgaaa ttagcactga
    34021 acccggtcct cactttggcc tcggtctgga agcatacgcc acctggactt cgccgatccg
    34081 taaatatggc gacatgatca accaccgtct gctgaaagcg gttatcaaag gcgaaactgc
    34141 gacgcgtccg caggatgaaa tcactgtcca aatggccgaa cgtcgccgtc tcaaccggat
    34201 ggcagaacgt gatgttggcg actggttata cgcacgcttc ctgaaagata aagccgggac
    34261 cggcacccgt ttcgcggcgg aaattgtcga tatcagccgt ggcggcatgc gtgtgcgttt
    34321 ggttgataac ggcgctatcg cctttattcc ggcacctttc ttacacgctg tgcgcgatga
    34381 actggtttgc agccaggaaa acggcactgt acaaattaaa ggtgaaacgg tttacaaagt
    34441 aactgacgtt attgacgtca ccattgccga agtccgcatg gaaacccgca gcattattgc
    34501 gcgcccggtc gcgtaatctc ctttcacggc ccattcctca tggatgggcc gtttatttcc
    34561 ccgctctacc ttcgttatat ccaggcagat tttttaactg actttcgttt gaaaactggc
    34621 gtttttccaa aactggatta caaataaata tataaaaaac aatgcgttta gccgccgtta
    34681 atttcatgta agtcatgttg ttccttttat ttaaccgcgg actccgctgt taaccggagg
    34741 atatgcatca tgaaaaccgt tagggagtcc acaacgttgt acaactttct cggatcgcac
    34801 aatccatact ggcggttgac ggaaagcagc gatgctttgc gcttttctac caccgaaacc
    34861 acagaacctg atcgtacatt gcagttatct gccgaacagg ctgctcgcat cagggaaatg
    34921 acggtcatca cctccagcct gatgatgagt ctgaccgtcg atgaaagcga tctttctgtg
    34981 catctggtag gacgaaaaat caataaacgg gaatgggctg gcaacgcgtc tgcatggcat
    35041 gacacaccgg cggttgctcg tgatttatca cacgggcttt cctttgctga gcaggtagtt
    35101 tctgaagcac attccgcaat agtgattctc gacagccggg ggaatatcca acgcttcaat
    35161 cggttatgtg aagattacac agggttgaaa gaacacgacg tcattgggca aagcgtgttt
    35221 aaactgttta tgagccgtcg tgaagctgcg gcatccaggc gcaataaccg tgtatttttt
    35281 cgaagcggca atgcatatga agtcgaactg tggataccaa cacgtaaagg ccagcggctg
    35341 tttctgtttc gcaataaatt tgtccacagc ggcagtggca aaaacgagat ttttttaatc
    35401 tgttccggta ccgacattac cgaagagcgc cgcactcagg agcgactgcg tattctggca
    35461 aataccgaca gtatcaccgg actgccgaat cgtaacgcaa tgcaggagtt aatcgatcac
    35521 gctattaatc aggcagataa caataaagtt ggggttgtgt atcttgattt ggataatttt
    35581 aaaaaggtca acgacgccta tgggcatttg tttggtgacc agttattacg cgacgtgtca
    35641 ttggctattt taagctgtct cgaacatgac caggtgttgg cgcgtccagg tggcgatgag
    35701 tttctggtac tggcatccaa tacctcacaa agcgcgctgg aagcaatggc atcacgaatt
    35761 ttgacccgct tacgcctccc ctttcgtatt ggtttaattg aagtttatac cagctgttca
    35821 gtaggtattt cgctctctcc cgaacatggt tcggacagcg cgactattat tcgtcacgcc
    35881 gacacagcaa tgtacacagc gaaggaaggc ggacgaggac aattttgcgt ttttactcca
    35941 gaaatgaatc agcgggtatt tgaatatctc tggctggata ccaacttgcg taaagcactg
    36001 gaaaacgatc agttggttat tcactatcaa ccgaaaatca cctagcgtgg cgaagtgcgc
    36061 agtctggaag cactagtacg ttggcagtca cctgaacgtg ggttgattcc accgttggac
    36121 ttcatttcct acgccgaaga gtcagggcta attgtgcctt taggccgttg ggtgattctc
    36181 gatgtcgtac gccaggtggc aaagtggcgg gataaaggca taaacctgcg agtggcggta
    36241 aatatttctg cacgtcagct cgccgatcaa accattttca ccgccctgaa acaggttctc
    36301 caggaactca attttgaata ctgccctata gatgttgaac tgacagagag ttgtctgatt
    36361 gagaatgatg aactggcact gtctgttatt caacaattta gccaactagg tgcgcaagtg
    36421 catctggatg attttggtac cggctactct tcactttcgc aactggcgcg ctttccgatc
    36481 gatgccatca aacttgacca ggtttttgtt cgagatattc acaaacaacc tgtctcgcag
    36541 tcactggtcc gggcgatcgt cgctgtggcc caggcattga atcttcaggt gatcgccgaa
    36601 ggtgtagaga gtgcaaagga agatgctttt ttaaccaaga acgggatcaa tgagcggcaa
    36661 ggatttttgt ttgccaaacc gatgcccgcc gtcgccttcg aacgctggta taaatgctat
    36721 ctgaagcgcg cataagtaga tgaatatata tacttagcgc tatatcccgt cattatatat
    36781 cgtattcctg gatgataatt agccgttgct gttaaacact tctcttttag cggcaatttt
    36841 tgcattattc attttccccc ttttgctata aggatgtcct tgatgtctga atttgatgcc
    36901 cagcgcgttg ccgaacgtat tgatattgtg ttagatatcc ttgtggcagg cgattaccac
    36961 tctgcgatcc ataatctcga gatacttaaa gcagaactct tgcgccaggt tgctgaatct
    37021 acgccagaca tcccgaagac accgtgggag atttaacaat cgtgccttca ggttttccta
    37081 tttgaggatg cccggcgcgt cagtattttt ttatttagta ttataacgtt ataagaatta
    37141 cagcgatgaa ttcccgacaa caaactattc tacagatggt cattgaccag ggtcaggtta
    37201 gcgtaaccga tctggcaaaa gccactggag tttctgaagt taccattcgc caggatctca
    37261 acaccctcga aaaactgagt tacctccgcc gtgcacatgg ctttgcagtt tcgcttgata
    37321 gtgatgacgt cgaaacccgt atgatgagca actatacgct gaagcgtgaa ctcgccgagt
    37381 ttgccgcgtc actggttcaa ccgggcgaaa ccatctttat cgaaaatggc agcagcaatg
    37441 ccctgctcgc ccggactctg ggggagcaga agaaaaatgt cactatcatc acggtcagca
    37501 gctacatcgc gcatttgctg aaagacgcgc cttgtgaagt cattttgctc ggtggcgtgt
    37561 accagaaaaa aagcgaaagt atggttggcc ctttgacacg ccagtgcatc cagcaggtac
    37621 atttcagcaa agcatttatt ggtattgatg gctggcaacc tgaaactgga ttcaccggtc
    37681 gcgatatgat gcgcaccgat gtggtcaatg ccgtgctgga aaaagagtgc gaagcgatag
    37741 tcctgactga cagctcgaaa tttggtgctg tacattcata ctccatcggt cccgttgagc
    37801 gattcaatcg cgtgattacc gattcgaaaa tacgcgccag cgatctgatg catcttgagc
    37861 acagtaaact caccgttcac gtcgttgaca tttaaccatt tcccttctct acggatgatt
    37921 tgcagtttgg caaatcatcc gctctaagat gattcctggt tgataattaa gactatttac
    37981 ctgttattaa cactctcaag atataaaatt attatcagcg atataacagg aagtaattat
    38041 cacctgcgtg atataaccct gcgcgcgagc agatttcacg gaataatttc accaggctta
    38101 ttcttagcta ttatagttat agagagctta cttccgtgaa tcataaattc aggagagagt
    38161 attatgtttg taacgagcaa aaaaatgacc gcggctgttc tggcaattac tttggcaatg
    38221 tctctgagtg cctgttctaa ctggtctaaa cgggaccgca acaccgcaat cggcgcgggt
    38281 gcaggggcat taggcggtgc agtactgacc gatggcagta cgttgggtac attaggtggt
    38341 gcagctgtcg gtggtgttat tggtcaccag gtcggtaaat aatcgtaaat aaatgtcgat
    38401 tatcgactcg caattataat acgcttgatt ctgttcagta cgaaataact gaataataca
    38461 cacagtaaaa tattcgaaag ccacggatat atccgtggct ttttatgtta accgcctgcg
    38521 agttttacct tcatcccttt cgcttcaagt agtgatttta ataaatcacg cttatcgccc
    38581 tggatttcaa taactccatc tttaactgct ccgccgcagc cgcatttttt cttcagttcc
    38641 gctgcaagtt ttgtcagttc ggcatcatcg agatcgatac cggtaatcag gcaaacgccc
    38701 ttacctttac gtccactggt ctgacgctga atacgcaccg ctccatcgcc tttagggcgt
    38761 actggggccg ctttgggctc atcaatacgc ccggtttccg ttgagtagac cagacggctg
    38821 ttggaatcac tcatcatgca ctccgctgta aagaggcgtt gatcgctttc agtgtctgcg
    38881 ctggatctac cgattgcgtt accgggcgac caatcaccat ataatcaaca ccagccgcca
    38941 acgcctgttc tggcgtcata atgcggcgct ggtcaccagc ttcactcccc tgcggacgaa
    39001 tgcccggtgt aaccagtttg aactcctgac cgaatacctg tttaaagcgc acagcttcct
    39061 gagcagaaca caccacacca tcaaggccac atttttgcgt cagtgctgcc agacgttctg
    39121 cataatctgc aggtgacagt gtcacgccaa gatcggccag gtcgctggct tccatgctgg
    39181 tcaacactgt cacagcaatc aaaagcggtg catctttgcc aaacggaacc agtgcctcac
    39241 gcgctgcggt catcatacgc gccccaccgg aggcatgaac attcaccatc cacacgccta
    39301 agtcagctgc agcagcgaca gcgtgcgctg cagtgttggg gatatcgtgg aatttcaggt
    39361 caagaaagat atcaaaacca cgctgttgaa gttcgcgcac aaactgtggc ccaaacaatg
    39421 taaacatctc tttaccgacc ttcagacgac aatcgcgtgg gtcgatcttg tcgacaaagg
    39481 acagcgcgtc atcacaatta tgataatcaa gggcaacaac cacaggagaa ttcgtaacag
    39541 cgcgggaaga agatgaagca gttaacgtca tgaccagacc ttcttgatga tgggcaccgg
    39601 aagtggcgcg aaacaggtaa acggcgagca ttctacccgt ggaaataaaa aattaacagt
    39661 tgcgattttt cccctagcag gctttttctg ctccgtcgtt gccggagcac aatgggacat
    39721 caataactag tatgttgtaa ctaaagtcgg atttttttaa aaattacagg ccatcaagac
    39781 cgcgaatcgg tttaatagtt gaccaggcac gacaagacgg acaatgccag tagagggtgt
    39841 atgcggtaaa accacatttc tggcagctat aacgaggctt actacgcacc ttctcgccaa
    39901 ccatgtcacg cagcaccatc agactctctt tggcgcgccc ttcttccgct tcatttaagt
    39961 ggtaatccat tagcttatgg aatacacgca tggtcggatg acgctgaagc tggcgtgtaa
    40021 tatagacctg tgcagcctca ctaccgtcgc gcgcttcgat gatatcagca agcatcaatt
    40081 cagcatcggc accggtgttc tcttccaccg cgcgttgcag gaattctgcc cattcggcag
    40141 ttttacccaa ctgctgatag caggtttgca acatttccag cgtttcgctg accagttctc
    40201 tgtcctggga tatgacgcgt tgcaaacttt cgacggcttt ggcgtattct cctttcgcca
    40261 taaacacgcg tcccatcatg atggatacgc gggcgctgtt tttatctgcc gccgcccctt
    40321 tttttagcaa ggtcatggca cgatcgagat cgtcgctggc catatgctgc agggctaact
    40381 cacagtagaa atgggcaatt tcgacgcgct gtttatcttt acccagcttc accaggcgtt
    40441 cggcaacatc aattgctttc tgccactcac tggtagcctg gtagatttgt agcaactgtt
    40501 gcagcgcgcc aatgcggaag tcagtttcat cggtcagctg attgaacatg tcttccgcgc
    40561 ggtcgtataa cccggcggcc atgtaatcac gccccagttg ttgaatcgcc aacagacgct
    40621 gttcataggt cagcgaggcg ctttccatta gggtctgatg gatgcgaata gcgcgatcaa
    40681 cttcgccacg cgaacggaac aggtttccga gcgtaaggtg ggcttcaacg gtgcctgtat
    40741 cctctttaag catatcgaga aacaggtcta ccgctttatc ctgttgatta ctaagcagga
    40801 agttaacccc cgctacgtaa tcacgcgaca agcggttggc ttcatcttgc ttgttttgtt
    40861 gcgcacttct gcggcccata taccagccat aggcagcggc tacaggcaaa agcagaaaca
    40921 acaactccag catagaaagt tattccttcg ccgctgacga gtgcggcact acagccacgt
    40981 cagtcgcggg tgaaagctgg ttttccagtc gctttatttt acgttcagcg cgcgccaggg
    41041 aaacacgaac tcgcagccag aacaggccac aaatcaacca accgatagca aaccccgcag
    41101 caaacaatac cgccagcaat gtggaaatac ggtactcccc ttgcgctaac agataattaa
    41161 acgtcacctg ttgatcgttc tgcgcaccca acgtgaccga aatcacgaag atcgctaaca
    41221 ccagtaagaa aatgagtaaa tatttcacat tacttcccgt tatgtggtta gagcgaataa
    41281 attatggtca acttaccgca atagcccttt aaagtaccat tttcgcgccg tctgcgaaat
    41341 ggcatccacg cgcattgttg atgatttaag gcctaaattc ggaaaatcaa ccagcgctaa
    41401 ctttcttgtt ctcgttgtgc aatttcgcga ttttcttccg caggtggtgt taatggccca
    41461 caaattcgtt gcgcaagcca cgttgccacc gccaccagcg cccacgaaat caacgtagct
    41521 accaccagat cgcgtggcca atgcatcccg agcagcaggc ggcttcccat gactcccgtt
    41581 gcccagacca acaagatagc aatggttaac gttcgccgac gcggccacag caaaccaacg
    41641 gccagcagtg cccaactggc agcaaacatc gtgtgaccgg aaggaaaggc aaaccccgtc
    41701 tctttctgcc agtgtgaacg caaatattgt ggaatatttt tctcttcagc caactgttct
    41761 tttactagat ttccgcgttc tgctcgcttt aaagtgtaga actcatcaac cggaatatga
    41821 tgtatttttt ccaaccagat aacaaaaggt cgtggttcct ggactttgtc tttgatccag
    41881 gatttaacgc cttgtccaac aaggattgcc gccgccagaa tggcaaataa tacaaaggca
    41941 gccttaatgc gaaaacgcag acaccagaga aaccagccga acaaaatcaa atgtgtaatg
    42001 acgccccagg gctgggtgac agtttcagta acccaaaaag ccgcttttag tagccaactt
    42061 tgttctccag gttgccaacg ccagccagaa atccatacgg ctactggcat gacaagcaat
    42121 agtgcagctc ccactgcggt acgtcgggca atcgaacgca tggcctctcc tttttgataa
    42181 gtcccacaat cataactgaa aatgccagtt ccaagaaaaa ttgacagatt tgtgccattc
    42241 cgtgaacgat cgacgcgtcg tgattaggtg aaccccttct cgttatggca aaataagcca
    42301 atacagaacc agcattatct ggagaatttc atgcagctta aacgtgtggc agaagccaaa
    42361 ctgccaaccc catggggcga tttcctgatg gtgggatttg aagaactggc aaccggacac
    42421 gatcatatcg cgctagtcta tggcgatatt tccgggcata ccccggtact tgcgcgcgtc
    42481 cattccgaat gtctgaccgg tgacgccctg ttcagcttgc gctgcgattg tggcttccag
    42541 ctcgaagcgg cattgacgca aattgccgag gaaggccgtg gtattttgct gtatcaccgt
    42601 caggaaggtc gtaacattgg tctgctgaat aaaatccgcg cttacgcact gcaggatcaa
    42661 ggttacgata ccgtagaggc taaccaccag ttaggcttcg ccgctgatga gcgcgacttc
    42721 actctttgcg ctgatatgtt caaactcctt ggcgtcaatg aagtccgctt gttaaccaat
    42781 aacccgaaaa aagtcgaaat tctgaccgaa gcagggatta atattgttga acgcgtacca
    42841 ttgattgtag gtcgtaaccc caataacgaa cattatctcg ataccaaagc cgagaaaatg
    42901 ggccatttgc tgaacaaata accctcttgc attgtgtaat tcatttgctt gccggaagca
    42961 aaataaccgg caagcaaata cttgttactt caacatatta cgaatgacat aatgcaaaat
    43021 gccgtcgttc tggtagtagg tcaactccgt cgcggtgtcg atacgacaac ggcagggtac
    43081 gacttcctgg ctaccatccg cgcgcgtaag cgtcaccgga accgtcgcgc caggttgtag
    43141 attttgcaga tcgccaatat caatcttctc ttccccggtt agccctaacg ttttacgcgt
    43201 cacgccttgc ggaaattcca gcggcaggat gcccatgcca attaaattcg aacggtgaat
    43261 tcgttcaaac gattcggcaa tcaccacacg aattccaagc agacgaggtc cttttgccgc
    43321 ccagtcacgg cttgagcctg atccatactc tttcccggca atcaccgcca gcggcgtttg
    43381 ctcctgctta tagcgcatcg cagcatcata aatagagact acgtcgctgt caggtaaatg
    43441 ccgcgtcatc cccccttcaa cgccaggcac catttcatta cggatgcgaa tattggcgaa
    43501 ggtgccgcgc atcatcactt catggttacc acgccgcgaa ccgtaggagt taaagtcttt
    43561 tcgctcaaca ccccgacctt gtagatatcg acccgctggg ctgtcgggct taatactgcc
    43621 cgccggagag atatggtcag tggtgactga atcccccagc attgcgagga tccgcgcacc
    43681 gtgaatatct tccactggtg ctggtgttgc ctgcatttca tcaaagaaag gcgataagcg
    43741 aatataggtt gagtcctcct gccaaccgta ggtatcggat cgtgtgacgt taatttcctt
    43801 ccactctgct gtgccttcaa aaacttctgc gtactctttg cggaacattt ctgtggagac
    43861 ttgttctacc gcacgggcaa tttcttgtgc cgatggccag atatctttca gataaaccgg
    43921 atcgcctttg cgatcatggc cgataggctc agaagccagg ttgatattca tatttcccgc
    43981 cagcacatag gcaaccacca gcggcggcga ggccagccag ttagttttaa ccagcggatg
    44041 gatacggcct tcaaagttac ggttgccgga cagcaccgca ccgacggtta aatcgccttt
    44101 tttgattgct gtttcgatag gatcgggcag cggcccagag ttaccaatac aggtggtaca
    44161 accgtatccc acaaggttaa accccagttc gtcgagatac ggtgtcagtt tcgcttttgc
    44221 cagataatca gaaacgactt tcgaacccgg tgccagcgac gctttgaccc acggttgccg
    44281 cttgaggcca agagttacgg cttttttcgc cagcaagcct gcggccatca gcacacttgg
    44341 gttagaggtg ttggtgcacg aggttatcgc agcaatgacc acagcgccat caggtaactg
    44401 atactgatgt ccgttcataa cataatcgac tggcaggcga tctttatgcg tggcattcac
    44461 ttccagttcg ttactggcgg caaatgcttt tggtacatcg ggcagtgcaa cgcgatcctg
    44521 tgggcgtttt ggccctgcca ggctcgcttc aacgtcattc atatccagtt ctaacatact
    44581 ggtaaaaatc ggttcatcgc ccgggttacg ccacatgccc tgcgctttgg catatttttc
    44641 gaccaactcg acctgatctt cgctgcgccc gcttaaacgc atgtaatcga gggttactgc
    44701 atcgattggg aagaagccac aggtggcacc atattctggc gacatattgg caatggtggc
    44761 gcgatccgcc aacggtagtg aatccagacc atcaccataa aattcgacga atttccccac
    44821 cacgccatgt ttgcgcagca tttgggtaac agtgagaacc aggtctgtgg cggtaatacc
    44881 ttcacgtaat tttccggtaa gtttaaagcc cactacgtcc gggataagca tggaaaccgg
    44941 ctggcctaac atggcggctt ctgcttcgat cccacccacg ccccacccca gcacgccaag
    45001 gccgttgatc atagtggtgt gcgagtcagt accaacgagt gtatccggat aagcaatcca
    45061 ttcaccgtcc tgcaattcgc tccacactgc tttgccgaga tattcgaggt taacctgatg
    45121 gcaaatgcct gtgcctggcg gcacgacgct aaaccgactg aacgcttgct ttccccattt
    45181 caggaacaca taacgttcgt ggttgcgctc catttccagg cgtacgtttt cttcaaatgc
    45241 ctcatcatca ccaaaacgat cgacggtcac cgagtggtca atgaccaggt cgaccggtga
    45301 gagcgggtta acctttgcag tatcgccacc gaggcgttta accgcttcgc gcattgccgc
    45361 cagatcaaca acggcaggta cgccggtaaa gtcctgcatc agcacccttg ccgggcggta
    45421 ggcaatttca cggtcagcat gggcattttt cagccatccc gccagtgcgt ggatatcctc
    45481 ttcggtaact gagttaccat cctgccagcg cagcaggttt tcgagcaaaa ctttgagtga
    45541 cttgggtaga cgggtgatat cgcccagtga tttagcagca agcggcaggc tgtagtagtg
    45601 gtaagtttta tctttggcct gcaacgtgtc cttactggct tctcgtaggg ttgacgacat
    45661 agctcctcct taatgacagg gttgcgaagc cctgattctc ttcagggtga atattaaaga
    45721 taacacaaac atcgcgtaac gatttgataa caacccaaat tgataaaaga ggagaaacct
    45781 tgtggcaaag caaatgacaa ccccgccgca gcggggtcaa ggatcagtga gtaatgatcc
    45841 agaggagttc ggcccagaag agcatcgaca gagaaaacgc gcccatccag gaccaatatt
    45901 tcatatttgt gttgatcata ggctacaccc ttaacggtta ttattttgtg ttgagataac
    45961 tcaagctgtt attgctgcgg tgtattaaaa gccctttaat tcattaaaaa tgggtaataa
    46021 gaatgatgat aattattgtt tcggctgatc atcttcagca aacagttcta taaaggcttg
    46081 ctgctgcgat gttagttgcc agtcgtctgg aataacgacg gaacattccc gtttcgtttc
    46141 tggttcattg tcactatgct ggcataatgg tgacgactcc agttgctcct gaccgaccat
    46201 agttatcccc acactttcca aatcagcagt gcgacaacca cccagaaaag tgcagaacca
    46261 agaaaaaccg ccagccaggc tttacgtttt aattcaggat cacggcgggg ttcctgattt
    46321 ccagaaggca ttgctaacct catataatcg ccatcactta tcagcaagac gttgaatgat
    46381 tggtgtaatt aatcatacga tgagacaaat cctaaagaaa ctttagtcaa aaatccagcg
    46441 aatttacgaa tcgtctccag tgaataattc aatcttttga cctgaaatag atatttgaaa
    46501 actggaattt gcgaggcggg taattagaca ctactttaca ggtaatttac cctataaata
    46561 tgagggataa aaatattgtg cacggtatag aaataagtaa caaaagagaa tatattccgg
    46621 caccttcgct acataaaagg tgccgaaaat aacgcaagaa attatttttc cggcagtttt
    46681 atatctttaa acatgacctc aatttcttca ttggagcgca atgcgacagc cgcatcaacg
    46741 acatcacgcg ttaaatgcgg tgcaaaacgc tgaatgaaat catacatata actgcgcaaa
    46801 aaagtactac ggcgaaaacc aattttggtt gtactgtggc tgaagatatc gtgagcatca
    46861 acgcgcacaa ggtcgggatc ggcgaccgga tccaccgcca tgctggcaat gacccctacc
    46921 cccagcccta accggacgta agttttaatg acgtcagcat ccgttgccgt gaaaacgata
    46981 cgcggcgtta accctgcgcg gttaaaggca gtatccagtt ctgaacgtcc ggtaaagccg
    47041 aaggtatatg tcaccaacgg atattgcgcc agttcttcaa tggtaatggc ttttttgcct
    47101 gccagcgggt gatccggagt gactacaata gcccgattcc agtggtagca cggtaacatc
    47161 actaaatctt catacagatg cagcgcttct gtggcgatag cgaaatcggc attgccttta
    47221 gagacggcat cagcaatttg tgtcggcgag ccctggtgca tatgcaaaga aacgcgggga
    47281 taacgctcaa taaagccttt gataacgttt ggtaacgcgt agcgtgcctg ggtatgcgtg
    47341 gtggcgatat acagcgagcc tttatccggc caggtgtgct caccggcaac cgattttatg
    47401 gcatcgactt tcgacaggac ttcgcgagcg atacgaatta tttcttgtcc tgctggcgtt
    47461 acctgcgtca ggtgcttgcc gcttcgggaa aaaatttgaa tgcctagctc gtcttccagc
    47521 attctgactt gtttactgat cccgggttgt gatgtgtaaa gtccttccgc tgttgatgag
    47581 acattcaggt tatgattgac cacctcaaca atatagcgaa gttgttgtaa tttcatgtta
    47641 aaccatccac ttagacgcca tcagaacatc gttttttcgt cacacctggc gggtgactaa
    47701 tacgatttaa taataaaaaa ggtgctaact ataaccacta tatcatttat agccttagtg
    47761 tataggtgtg aagaaaaaat aatatgcaat aaaaaagggc cgctttcgcg accctttgtt
    47821 tataaaaacc tgacagaatt aaaggttatt tttttccttc aacccatttg ccatcaacat
    47881 aaaatgctga ccagccagtc gcctttccgt ctttttccga agagacatat tgctgtttgg
    47941 ttttacggct aaagcgaacc atggtcttat taccttccgg atcctgctgt ggcgcatcgg
    48001 ccagataacg cagtttttcc ggcagacggt cgcggaagcg ataaagctct tccaccagtg
    48061 gcgcacgcgt ttcacgcgat ttcgggaaag tgttggcagc caggaacaca ccggcagcac
    48121 cgtcacgcag cacgaaataa gcatctgatt tttcgcacgg cagctcaggt aatggcaccg
    48181 gatcttcttt cggtggtgcc acttcgccgt tacgtaaaat cttacgtgtg tttttacact
    48241 cttcgttggt gcaggccatg tatttaccga aacgccccat tttcaggtgc atttcagagc
    48301 cacatttttc acactcaacg atcgggccgt cataaccttt aatgcggaat tcgccctctt
    48361 cgatctcgta accgtcgcag gttgggttat taccacagac atgcaactta cgtttcggat
    48421 cgatgagata gctgtccatc gccgtgccgc atttcgggca acgacgtttt gcgcgcagcg
    48481 cgttggtttc agcatcttcg ccttccagca cgttcagcac ttcgttttcc ggcaccaggt
    48541 taatggtggt tttgcaacgc tctttcggcg gcagcgcata gccagaacag ccaaggaata
    48601 ccccggtgct cgctgtgcga atccccattt tgcgaccaca agtcgggcag tcaatgctgg
    48661 tcagaaccat ctggttcggg cgcataccac cctcttccgg atctttttca gctttatcta
    48721 actgctgggt gaaatccgag aagaagtgat ccagtacagc tttccactct gcttcgtgat
    48781 ttgccacctg gtcgaggctg ttttccatct gcgcggtaaa gtcgtagttc attaactcgc
    48841 ggaaattttc ttcaaggcga tcggtgacga tttcgcccat tttttccgca tagaaacgac
    48901 gattttctac tcgcacatag ccacgatcct gaatggtcga aatgatcgac gcataggtag
    48961 acggacgacc gataccacgt ttttccagtt ctttaaccag cgatgcttca ctgaaacggg
    49021 ctggcggctt ggtaaagtgc tgggctggtg ttagttcaac gagcgtcaga gcatcgcctt
    49081 tatcgactgc cggtaagata cgatcttcat cgcctttacg cagtgcaggc atcacttttg
    49141 tccagccatc aaagcgcaaa atacgaccgc gtgctttcag gcggaaatcg cccgcaccaa
    49201 cggtcagcgt cgtggagtca tatttcgctg gggtcatctg gcaggcaacg aactgacgcc
    49261 agattaactg gtacagtttc tgcgcatctg cttccatatc cttcagcgat tccgccatca
    49321 cattgacgtc agaaggacga atcgcttcgt gcgcttcctg tgagttttct ttgctggcgt
    49381 actgattcgg actttccggc agatatttct taccaaaatt atcgctgata taaccgcgaa
    49441 ccatatttac cgcgtcctga ctcaggttag tggagtcggt acgcatgtaa gtgatatagc
    49501 ctgcttcata caaacgctgc gccatcatca tggttttttt cacgccaaaa ccaagacggg
    49561 tgctggcagc ttgttgcagc gtagaggtaa taaaaggagc gccaggttta ctggttgtcg
    49621 gtttgtcttc acgttccagc acgctgtagc gcgctttttc cagcagactt accgcagcct
    49681 gagtttgttc tttgttgacc ggacggaacg gtttgtcatt ctgatgagtc acctgcaacg
    49741 ccaacgcttc accagatggc gtggtcgtgc tggcatcgac ttcccagaac tcttccggta
    49801 cgaacgcttt aatttcacgc tcacgctcta ccaccaggcg gaccgccacc gactgcacac
    49861 gaccggcaga cagaccacga gcgatctttt tccacagcag cggcgaaacc atatacccca
    49921 ccacgcggtc cataaagcga cgcgcctgct gggcattaac acgatcaata ttcagctcac
    49981 ccggtttgtt aaatgcctgg cggatcgcgt ttttagtaat ttcgttaaac accactcggc
    50041 tatagcgcgc atcatcacca ccaatcactt cccgcaggtg ccatgcaatg gcttcccctt
    50101 cgcggtcaag gtcggttgcg agatagatgt ggtcggcttt ttcagccagt tgtttcagtt
    50161 cagagacgac cttctcttta ccaggcaaca cttcatagtg cgcctcccaa ttgtgccacg
    50221 ggtcaacccc catacggttg acgagagcgc cacgttcatc ctttttaggc tttttagccg
    50281 tcttggtgga ggtagagtcg gcactctttt tggcagctga gccactggtc ggcaaatcgc
    50341 ggatgtgacc gacgctggat ttcaccacgt agtcactacc cagatacttg ttgatcgttt
    50401 tggcttttgc cggggactca acgatgacaa gagctttacc catattcacc ttacctaatt
    50461 tgattcttcc aggaatgcgt cgcacgttga taaaccttcc actggcaacg agttaccgat
    50521 atggactcgc tctctgcgga tatcaacccc ttgtcgtgac tctgttcacg aaaattcagg
    50581 tgactgagtt aacaggtctt ggcctacagg cgctatagca cgacggaatt ttggtcgaat
    50641 gtcaagcaaa tctattgcca gattgacgaa agcgtcacac tgtacctgat aaaatccgat
    50701 acgcaacttt attagcatgc aaaatccaaa gttgccagta aggcgctctt gccaccagcg
    50761 tggtatattt gtctgcaagc catgcctggc gtagactaat gtccttgttt aaggagtaaa
    50821 taatgaacaa agagacacaa cccatcgacc gcgaaacgct gttgaaagag gccaacaaaa
    50881 tcattcgtga gcatgaggat acgctcgcag ggattgaagc caccggcgtc acgcagcgta
    50941 atggcgtgct ggtatttacc ggcgactact ttttagatga acaaggattg ccaacagcca
    51001 aaagcaccgc ggtgtttaat atgttcaaac acctggccca tgtgctttca gaaaaatatc
    51061 atctggttga ttaaagcaaa acgcgaggtc ttagcctcgc gtttgtcttt tacatcaatg
    51121 gcttttgacc ccgctgccac cagcgtagca acaatcgatc ggcgctctct gccgcgctgc
    51181 cggtgaatcg gtcaatgagt cgtttacgct gcatatagcg tacattaacc acttcacggc
    51241 cttccatcag gctaagaata acttcatcac tggtgttgat ttcatcaacc aggcctttct
    51301 ctaccgcctg ttgtccgtac cagtgttcac ccgttgccac ctgttcaata tccagagacg
    51361 gacgcatacg cttcacaaaa tctttaaata actgatgcgt ttcgttcagc tcttcgcgga
    51421 atttctcccg cccttcttcg gtattttcac ccagcaaggt cagcgtgcgc ttatactgcc
    51481 cggcggtgtg cagttcgata tcaatatctt tgcttttcag gaagcggtta aagttgggca
    51541 tttgcgccac caccccaatg gaacccacaa tagcaaacgg tgcggaaaca attttgtccg
    51601 ccacacaggc catcatgtaa ccgccgctgg cagcgacttt gtctaccgta acagttaaag
    51661 gaatgttttt atcacgcaga cgctgcagct gcgaagccgc caacccgtaa ccatgcacca
    51721 tgccaccagg gctttccaga cggagcacaa cctgatcctg cggtttgaat gctgcgagta
    51781 ccgccgttat ctcttcacgt agcgagttca cttcatgggc gtccatgctg cctttaaaat
    51841 ccagcaccca gacgcgaggt ttactgtcag ttgccacctc gcccagtttg gctttcgctt
    51901 ttgctgcttt cgcttcttgc ttgtgctttt tcttctgcgc tttgtgccac tgtttttgct
    51961 gatgtgagtc catcagcgcc gcggccagtt cttctttcat ctccttatac tgttcgctga
    52021 gattgttgac ccgtaactcg ccacgctggc gtttattacg ttgagcaaca ttgacaataa
    52081 tggcggcaat cgccgcaatc gctagcacaa cggtaacgat tttcgccaaa aacaaaccat
    52141 attcagacaa caattccacg agtccccacc ttggttaaac aacgctttgg ctcacagttt
    52201 acaacaggca tgttaagccg tctcgcagag atgttattgc agcgaaagta tgacaaagta
    52261 tccattttgc ggtgttatcg ccccaggctg attgaaatgc ccttctgttt caggcataaa
    52321 gccccaaagt cataaagtac actggcagcg cgacagacaa cgtcgttgag gagtagccat
    52381 gcattaccag ccaaaacaag atttactcaa tgatcgcatt atcctggtga cgggagccag
    52441 cgatgggatt ggtcgtgaag ccgcgatgac gtatgcacgc tatggtgcga cagtgattct
    52501 gttgggccgt aatgaagaaa aattacgtca ggtagccagc cacataaacg aagaaactgg
    52561 gcgtcagcca cagtggttta ttctcgattt gctgacctgc acgtccgaaa attgccaaca
    52621 actggcacag cgcattgtcg ttaattatcc gcgtctggat ggtgttttgc ataatgccgg
    52681 attgctcggc gatgtttgcc caatgagcga acaaaatccg caggtctggc aggacgtcat
    52741 gcaggtcaac gttaatgcca cctttatgct cacccaggca ctgcttcctt tattactcaa
    52801 atcggacgcc ggttcactgg tctttacttc atcaagcgtt ggacgtcagg gacgagccaa
    52861 ctggggtgca tatgcagcgt cgaaatttgc caccgaaggg atgatgcagg tactggccga
    52921 tgaatatcag cagcgcctgc gcgtcaactg cattaaccca ggcggtacgc gcaccgcaat
    52981 gcgcgcaagc gccttcccga ccgaagatcc acagaaactt aaaacacccg ctgatatcat
    53041 gccgctctac ctctggctga tgggcgatga cagccgccgt aaaaccggca tgacctttga
    53101 cgcccaaccg ggccgtaaac caggaatttc ccaatgagtg atgaacgcta ccaacagcgt
    53161 cagcagcgag tgaaagaaaa agtagatgcc cgtgtggccc aggcccagga tgaacgcggt
    53221 attatcatcg tctttaccgg caatggaaaa ggcaaaacca ccgcggcatt tggtacggca
    53281 acacgcgcag ttggtcacgg aaaaaaagtg ggcgtcgtgc agtttattaa aggcacctgg
    53341 cctaatggcg aacgcaatct gctggagcca catggcgttg agtttcaggt gatggcaacg
    53401 ggctttacct gggatacaca aaaccgcgag tctgataccg ccgcctgccg caaagtctgg
    53461 caacatgcaa agcggatgct tgctgattcc tcactggata tggttttgct tgatgaactg
    53521 acgtatatgg tggcgtatga ctatttgcca ctggaagaag tggtgcaggc gttaaatgaa
    53581 cgtccacatc aacagacggt gattatcacg ggtcgaggct gtcatcggga tattcttgaa
    53641 ctggcggata cggtaagtga gctgcgcccc atcagacatg cgtttgatgc cggtgttaag
    53701 gcgcaaatag ggattgatta ttaatccgga tatgacgtcc ggcatacttt gattaaccgt
    53761 tattacgccc gccagaacga cgaccgccgc tcacctgact gtgacgtttc accgcacgac
    53821 gaatctgatt agccttcatg cgacgacggt ctttttctac agcgacttta gagctggttt
    53881 ctggcggaag ctccaccagt tcgcgcagat agttagtctg ggcgaggtcc agctccgtcc
    53941 agccaccgcg tggcagacct tttggcagtg ggatatcacc gtaacgaaca cggatcaggc
    54001 ggctcacctg cacaccaacc gcttcccaca gacgacgaac ttcacggtta cggccttcgg
    54061 tcagagtcac gttgtaccac tggttgatcc cttcgccgcc gctgaacttg atggttttaa
    54121 aagctgccgg accatcttcc agttgcacgc cacgactcaa atcacgcagt ttcgcgtcgt
    54181 caacctgacc aaatacacgc acggcgtatt cacgttcaac ttcacggctt ggatgcatta
    54241 aacggtttgc cagttcacca tcggtggtga acagcagcag accacaggtg ttaacgtcca
    54301 gacgacctac ggcaatccag cgtgcgccac gcagctttgg cagacggtca aacaccgtcg
    54361 gacgcccttc cggatcgtta cgggtacaca actcaccttc cggtttgtaa taggccagaa
    54421 cgcgacaaat ttgttcagcg gactcacgta ccgaaatcag atgaccatcg atacgaattt
    54481 tcagcccagg ggtaacttca acgcgatcgc cgagtttagc aattttgcca tcaacactca
    54541 cacgaccggc ttcaataata gattcgattt cacggcggga gccgtggcca gcgcgggcca
    54601 gcactttctg taatttttcg ctcatagagc ttccttttgg tgtcgccttc acaggcgtcg
    54661 aacaggagaa tcaatggcgc cgtatagcgc cattttaagg ccgcgtagta tacagagtta
    54721 cgcacttata agaaaggctt cacatcacct acgccttcac gcaccacgac cggcgtatca
    54781 tcggtaagat caataaccgt tgttggtttc tgcccgagat agccgccgtg aataatcaaa
    54841 tctacctgtt tttccagacg gtctttaatt tcttcgggat cggattcggt aaattcgctg
    54901 cctggcagca ttagcgaagt ggaaagcatc ggttcgccca gtgcttcaag caacgcctgc
    54961 gcaatcgggt tagaaggcac acgcataccg atggttttgc gtttctcctg caacagacgg
    55021 cgtggcactt ccttcgtccc cttcaggatg aaggtatagt tgcccggcgt gttgtttttc
    55081 atcaaacgaa acgcaacatt atccacgaat gaataggtcg agagttcaga aagatcgcga
    55141 cacatcaggg taaagttgtg accgtccggc agctggcgaa tacgacaaat acgctccatg
    55201 gcgtttttat cttcaatttt acagccgagc gcatagccgg aatcagttgg atagacaatc
    55261 accccgcctt tacgcacgat ctccaccgcc tggttgatca gacgttgctg cgggttatca
    55321 ggatgaatat aaaaaaactg gctcataatt ccctctctgt ggtgttctgc ggctgttccc
    55381 atagctgcca gacgccttca acgcctgcgg gcagccagag tttacgaccc agttcgatcc
    55441 acgggcatgg ctgatgaaaa tcagatcctt gtgatgccca taaatgatgc tgacgcgcaa
    55501 gtgtggccag ctgggtgcgt tcattgggcg attgctggca ctgcgcgact tccatcgcgt
    55561 cgccgtggtg ttcggcaaaa tgcgcaacca atcttttcag ccatttagca ctaagattgt
    55621 accgcccagg atgagctagc accgccttac cgccagaatg atgaatgaca tcaatagctt
    55681 gttctattgt acaccactgt ggcggaacgt atccggtttt cccgcgcgcc agatactttt
    55741 taaagacatc tgccattgaa ctggctttgc cgcactccac aaggaaacgc gcaaaatgac
    55801 cgcgcgtcac tgcgccgccc tgcgccagtc gttgtgcgcc ttccagcgcc ccaggtattt
    55861 gcgctttttc aagtctttcg gcaatttgct gcgcccgctg attgcgccgt tctgtctgct
    55921 gcgcgaggaa ctcacacatt agcggatgag taatatcaat attcagcccg acaatatgaa
    55981 tttcatgatt ttcccagacc gtggaaattt ccacgccggg aataagattc agcgccagtc
    56041 ccgaacgtga aatttcctct cttgctggcg cgattgcagc tgtggtgtca tggtcggtga
    56101 tcgccagggt gccgacgcgc atctcgactg cacggtgcac caatgcttct ggcgtcaggc
    56161 agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc
    56221 tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca
    56281 aatattctga aatgagctgt tgacaattaa tcatcgaacc agttaactag tacgcaagtt
    56341 cacgtaaaaa gggtatcgac aatgaaagca attttcgtac tgaaaggttg gtggcgcact
    56401 tcctgaaacg ggcagtgtat tcaccatgcg taaagcaatc agatacccag cccgcctaat
    56461 gagcgggctt ttttttgaac aaaattagag aataacaatg caaacacaaa aaccgactct
    56521 cgaactgcta acctgcgaag gcgcttatcg cgacaacccg actgcgcttt ttcaccagtt
    56581 gtgtgggaat cgtccggcaa cgctgctgct ggaatccgca gatatcgaca gcaaagatga
    56641 tttaaaaagc ctgctgctga tagacagtgc gctgcgcatt acagcattag gtgacaccgt
    56701 cacaattcag gcactttccg gcaatggcga agccctgctg gcactactgg ataacgccct
    56761 gcctgcgggt gtggaaagtg aacaatcacc aaactgccgt gtgctgcgct tcccccctgt
    56821 cagtccactg ctggatgaag acgcccgctt atgctccctt tcggtttttg acgctttccg
    56881 tttattgcag aatctgttga atgtaccgaa ggaagaacga gaagcaatgt tcttcggcgg
    56941 cctgttctct tatgaccttg tggcgggatt tgaagattta ccgcaactgt cagcggaaaa
    57001 taactgccct gatttctgtt tttatctcgc tgaaacgctg atggtgattg accatcagaa
    57061 aaaaagcacc cgtattcagg ccagcctgtt tgctccgaat gaagaagaaa aacaacgtct
    57121 cactgctcgc ctgaacgaac tacgtcagca actgaccgaa gccgcgccgc cgctgccagt
    57181 ggtttccgtg ccgcatatgc gttgtgaatg taatcagagc gatgaagagt tcggtggcgt
    57241 agtgcgtttg ttgcaaaaag cgattcgcgc tggagaaatt ttccaggtgg tgccatctcg
    57301 ccgtttctct ctgccctgcc cgtcaccgct ggcggcctat tacgtgctga aaaagagtaa
    57361 tcccagcccg tacatgtttt ttatgcagga taatgatttc accctatttg gcgcgtcgcc
    57421 ggaaagctcg ctcaagtatg atgccaccag ccgccagatt gagatctacc cgattgccgg
    57481 aacacgccca cgcggtcgtc gcgccgatgg ttcactggac agagatctcg acagccgtat
    57541 tgaactggaa atgcgtactg accataaaga gctgtctgaa catctgatgc tggttgatct
    57601 cgcccgtaat gatctggcac gcatttgcac ccctggcagc cgctacgtcg ccgatctcac
    57661 caaagttgac cgttattcct atgtgatgca cctcgtctct cgcgtagtcg gcgaactgcg
    57721 tcacgatctt gacgccctgc acgcttatcg cgcctgtatg aatatgggga cgttaagcgg
    57781 tgcgccgaaa gtacgcgcta tgcagttaat tgccgaggcg gaaggtcgtc gccgcggcag
    57841 ctacggcggc gcggtaggtt atttcaccgc gcatggcgat ctcgacacct gcattgtgat
    57901 ccgctcggcg ctggtggaaa acggtatcgc caccgtgcaa gcgggtgctg gtgtagtcct
    57961 tgattctgtt ccgcagtcgg aagccgacga aacccgtaac aaagcccgcg ctgtactgcg
    58021 cgctattgcc accgcgcatc atgcacagga gactttctga tggctgacat tctgctgctc
    58081 gataatatcg actcttttac gtacaacctg gcagatcagt tgcgcagcaa tggtcataac
    58141 gtggtgattt accgcaacca tattccggcg cagaccttaa ttgaacgcct ggcgacgatg
    58201 agcaatccgg tactgatgct ttctcctggc cccggtgtgc cgagcgaagc cggttgtatg
    58261 ccggaactcc tcacccgctt gcgtggcaag ctgcccatta ttggcatttg cctcggacat
    58321 caggcaattg tcgaagctta cgggggctat gtcggtcagg cgggcgaaat tcttcacggt
    58381 aaagcgtcga gcattgaaca tgacggtcag gcgatgtttg ccggattaac aaacccgctg
    58441 ccggtggcgc gttatcactc gctggttggc agtaacattc cggccggttt aaccatcaac
    58501 gcccatttta atggcatggt gatggcagta cgtcacgatg cggatcgcgt ttgtggattc
    58561 cagttccatc cggaatccat tctcaccacc cagggcgctc gcctgctgga acaaacgctg
    58621 gcctgggcgc agcagaaact agagccagcc aacacgctgc aaccgattct ggaaaaactg
    58681 tatcaggcgc agacgcttag ccaacaagaa agccaccagc tgttttcagc ggtggtgcgt
    58741 ggcgagctga agccggaaca actggcggcg acgctggtga gcatgaaaat tcgcggtgag
    58801 cacccgaacg agatcgccgg ggcagcaacc gcgctactgg aaaacgcagc gccgttcccg
    58861 cgcccggatt atctgtttgc tgatatcgtc ggtactggcg gtgacggcag caacagtatc
    58921 aatatttcta ccgccagtgc gtttgtcgcc gcggcctgtg ggctgaaagt ggcgaaacac
    58981 ggtaaccgca gcgtctccag taaatccggc tcgtcggatc tgctggcggc attcggtatt
    59041 aatcttgata tgaacgccaa taaatcgcgc caggcgctgg acgagttagg cgtatgtttc
    59101 ctctttgcgc cgaaatatca caccggattc cgccacgcga tgccggttcg ccagcaactg
    59161 aaaacccgca ccctgttcaa tgtgctgggg ccattgatta acccggcgca tccgccgctg
    59221 gcgttaattg gtgtttatag tccggaactg gtgctgccga ttgccgaaac cttgcgcgtg
    59281 ctggggtatc aacgcgcggc ggtggtgcac agcggcggga tggatgaagt ttcattacac
    59341 gcgccgacaa tcgttgccga gctgcatgac ggcaaaatta agagctatca attgaccgct
    59401 gaagattttg gcctgactcc ctaccaccag gagcaactgg caggcggaac acctgaagaa
    59461 aaccgtgaca ttttaacacg cttgttacaa ggtaaaggcg acgccgccca tgaagcagcc
    59521 gtcgctgcga acgtcgccat gttaatgcgc ctgcatggcc atgaagatct gcaagccaat
    59581 gcgcaaaccg ttcttgaggt actgcgcagt ggttccgctt acgacagagt taccgcgctg
    59641 gcggcacgag ggtaaatgat gcaaaccgtt ttagcgaaaa tcgtcgcaga caaggcgatt
    59701 tgggtagaaa cccgcaaaca gcagcaaccg ctggccagtt ttcagaatga ggttcagccg
    59761 agcacacgac atttttatga tgcgctacag ggtgcacgca cggcgtttat tctggagtgt
    59821 aaaaaagcgt cgccgtcaaa aggcgtgatc cgtgatgatt tcgatccggc acgcattgcc
    59881 gccatttata aacattacgc ttcggcaatt tcggtgctga ctgatgagaa atattttcag
    59941 gggagctttg atttcctccc catcgtcagc caaatcgccc cgcagccgat tttatgtaaa
    60001 gacttcatta tcgaccctta ccagatctat ctggcgcgct attaccaggc cgatgcctgc
    60061 ttattaatgc tttcagtact ggatgacgac caatatcgcc agcttgcagc cgtcgctcac
    60121 agtctggaga tggggctgct gaccgaagtc agtaatgaag aggaactgga gcgcgccatt
    60181 gcattagggg caaaggtcgt tggcatcaac aaccgcgatc tgcgtgattt gtcgattgat
    60241 ctcaaccgta cccgcgagct tgcgccgaaa ctggggcaca acgtaacggt aatcagcgaa
    60301 tccggcatca atacttacgc tcaggtgcgc gagttaagcc acttcgctaa cggttttctg
    60361 attggttcgg cgttgatggc ccatgacgat ttgcacgccg ccgtgcgccg ggtgttgctg
    60421 ggtgagaata aagtatgtgg cctgacgcgt gggcaagatg ctaaagcagc ttatgacgcg
    60481 ggcgcgattt acggtgggtt gatttttgtt gcgacatcac cgcgttgcgt caacgttgaa
    60541 caggcgcagg aagtgatggc tgcggcaccg ttgcagtatg ttggcgtgtt ccgcaatcac
    60601 gatattgccg atgtggtgga caaagctaag gtgttatcgc tggcggcagt gcaactgcat
    60661 ggtaatgaag atcagctgta tatcgatacg ctgcgtgaag ctctgccagc acatgttgcc
    60721 atctggaaag cattaagcgt cggtgaaacc ctgcccgccc gcgagtttca gcacgttgat
    60781 aaatatgttt tagacaacgg ccagggtgga agcgggcaac gttttgactg gtcactatta
    60841 aatggtcaat cgcttggcaa cgttctgctg gcggggggct taggcgcaga taactgcgtg
    60901 gaagcggcac aaaccggctg cgccggactt gattttaatt ctgctgtaga gtcgcaaccg
    60961 ggcatcaaag acgcacgtct tttggcctcg gttttccaga cgctgcgcgc atattaagga
    61021 aaggaacaat gacaacatta cttaacccct attttggtga gtttggcggc atgtacgtgc
    61081 cacaaatcct gatgcctgct ctgcgccagc tggaagaagc ttttgtcagc gcgcaaaaag
    61141 atcctgaatt tcaagcccag ttcaacgacc tgctgaaaaa ctatgccggg cgtccaaccg
    61201 cactgaccaa atgccagaac attacagccg ggacgaacac cacgctgtat ctcaagcgtg
    61261 aagatttgct gcacggcggc gcacataaaa ccaaccaggt gctggggcag gcgttgctgg
    61321 cgaagcggat gggtaaaacc gaaatcatcg ccgaaaccgg ggccggtcag catggcgtgg
    61381 cgtcggccct tgccagtgcc ctgctcggcc tgaaatgccg tatttatatg ggtgccaaag
    61441 acgttgaacg ccagtcgcct aacgtttttc gtatgcgctt aatgggtgcg gaagtgatcc
    61501 cggtacatag cggttccgcc acgctgaaag atgcctgtaa tgaggcgcta cgcgactggt
    61561 ccggcagtta tgaaaccgcg cactatatgc tgggcaccgc ggctggcccg catccttatc
    61621 cgaccattgt ccgtgagttt cagcggatga ttggcgaaga aaccaaagcg cagattctgg
    61681 aaagagaagg tcgcctgccg gatgccgtta tcgcctgtgt tggcggcggt tcgaatgcca
    61741 tcggcatgtt tgctgatttc atcaatgaaa ccaacgtcgg cctgattggt gtggagccta
    61801 gtggtcacgg tatcgaaact ggcgagcacg gcgcaccgct aaaacatggt cgcgtgggca
    61861 tttatttcgg tatgaaagcg ccgatgatgc aaaccgaaga cgggcagatt gaagaatctt
    61921 actccatctc cgccggactg gatttcccgt ctgtcggccc acaacacgcg tatcttaaca
    61981 gcactggacg cgctgattac gtgtctatta ccgatgatga agcccttgaa gccttcaaaa
    62041 cgctgtgcct gcacgaaggg atcatcccgg cgctggaatc ctcccacgcc ctggcccatg
    62101 cgctgaaaat gatgcgcgaa aacccggata aagagcagct actggtggtt aacctttccg
    62161 gtcgcggcga taaagacatc ttcaccgttc acgatatttt gaaagcacga ggggaaatct
    62221 gatggaacgc tacgaatctc tgtttgccca gttgaaggag cgcaaagaag gcgcattcgt
    62281 tcctttcgtc acgctcggtg atccgggcat tgagcagtca ttgaaaatta tcgatacgct
    62341 aattgaagcc ggtgctgacg cgctggagtt aggtatcccc ttctccgacc cactggcgga
    62401 tggcccgacc attcaaaacg ccactctgcg cgcctttgcg gcaggtgtga ctccggcaca
    62461 atgttttgaa atgctggcac tgattcgcca gaaacacccg accattccca ttggcctgtt
    62521 gatgtatgcc aatctggtgt ttaacaaagg cattgatgag ttttatgccc agtgcgagaa
    62581 agtcggcgtc gattcggtgc tggttgccga tgtgccagtt gaagagtccg cgcccttccg
    62641 ccaggccgcg ttgcgccata atgtcgcacc tatcttcatc tgcccgccaa atgccgatga
    62701 cgacctgctg cgccagatag cctcttacgg tcgtggttac acatatttgc tgtcacgagc
    62761 gggcgtgacc ggcgcagaaa accgcgccgc gttacccctc aatcatctgg ttgcgaagct
    62821 gaaagagtac aacgctgcac ctccattgca ggggtttggt attttcgccc cggatcaggt
    62881 aaaagcagcg attgatgcag gagctgcggg cgcgatttct ggttcggcca ttgttaaaat
    62941 catcgagcaa catattaatg agccagagaa aatgctggcg gcactgaaag tttttgtaca
    63001 accgatgaaa gcggcgacgc gcagttaatc ccacagccgc cagttccgct ggcggcattt
    63061 taactttctt taatgaagcc ggaaaaatcc taaattcatt taatatttat ctttttaccg
    63121 tttcgcttac cccggtcgaa cgtcaactta cgtcattttt ccgcccaaca gtaatataat
    63181 caaacaaatt aatcccgcaa cataacacca gtaaaatcaa taattttctc taagtcactt
    63241 attcctcagg taattgttaa tatatccaga atgttcctca aaatatattt ttcctctatc
    63301 ttctcgttgc gcttaatttg actaattctc attatcgact aattttaatg agtgtcgaca
    63361 cacaacactc atattaatga aacaatgcaa cgcaacggga gaaataacat ggccgaacat
    63421 cgtggtggtt caggaaattt cgccgaagac cgtgagaagg catccgacgc aggccgtaaa
    63481 ggcggtcagc atagcggcgg taattttaaa aatgatccgc aacgcgcatc tgaagcgggt
    63541 aaaaaaggcg gtcaacaaag cggtggtaat aaatcaggca aatcctgatt caccgttaat
    63601 tattaatatc agctgataaa gataattgca tctgcgggcc gtatggctcg cagtgatttc
    63661 actggagaaa aatatgaata tgaagaccat tgaagatgta tttattcaac tgctttcaga
    63721 tacctacagc gcagaaaaac aattaacccg ggcactggca aaactcgcaa gagcaacatc
    63781 aaatgaaaaa ttaagtcagg cttttcatgc gcacctcgag gaaactcatg gacagattga
    63841 acgaattgat caagttgtgg aatcagaatc aaatctgaaa attaagcgca tgaaatgtgt
    63901 ggcaatggaa ggtcttattg aagaagctaa tgaggtcatc gaaagtaccg agaaaaacga
    63961 agtgcgtgat gccgcactga ttgccgcagc acagaaagtc gagcattatg agattgccag
    64021 ttacgggaca ttagcgacgc tggctgaaca attaggttac cgtaaagcag cgaagcttct
    64081 gaaagaaacc ctggaagaag aaaaggccac cgacatcaaa ctgactgatc tggcccttaa
    64141 taacgtaaat aagaaagccg aaaataaagc ctgaaatatg aattttaact tttagtcatt
    64201 ttataaagag gacattttca tgaatcgtat tgaacattat catgactggt tacgtgacgc
    64261 ccacgcaatg gaaaagcaag ccgaatctat gcttgaatcc atggccagcc gtatagataa
    64321 ttatcctgaa ctacgcgctc gtattgaaca acatcttagt gaaaccaaaa accagattgt
    64381 tcaactggaa actattcttg atcgtaatga catttcacgt tcagtcatta aagattccat
    64441 gagtaaaatg gctgcgcttg ggcagtcaat cggtggtata ttcccttctg atgaaatagt
    64501 taaaggctct attagcggat atgtcttcga gcaatttgaa atcgcctgtt acacctcact
    64561 attagcagca gcaaaaaatg ccggtgatac agtctcaatt ccgatcatcg aagcgatttt
    64621 aaatgaggaa aagcaaatgg ccgactggct gattcagcat attccgcaaa caactgagaa
    64681 atttttaatt cgttctgaaa ctgatggcgt agaagcgaag aaataatccc ctttatacca
    64741 tgtccttatt gaccccgtat attacggggt cgtttttgtg cggaattaaa aacgatatcc
    64801 tgctgagaac ataaacaccc acggatcgag gcgtacgcta tcgtgttgct gtgcaccgcc
    64861 tagcttataa ttggcggtgg tatcgatatc catgtaccac actgacatgt taaccaacca
    64921 gtcacggtta atcagataat caacccccac ctgcccggca gctccccagg aatctttcag
    64981 actgagatcg gaaagccccg cctctttgcc atgatcgtta aatccattat caaagaaggt
    65041 ggtgtagtta ataccggccc caacgtaagg acggaatttg ctgctggcat caccaaaata
    65101 ccactgcgcc atcagtgttg gcggcagatg gtgaacggtt gcaatatcgc cggtcgcccg
    65161 ggtgccgatt ttatggcgga acggcgtcgc tgccagtaat tccacaccaa tgttgtcggt
    65221 cgccatataa gtaaacgtca agcccagttg agtgttattg gtcacgctga atccacccag
    65281 acttcctaac gtaccaccag caccttctgt tggacgtacg gttgcagaac ctgcacgcat
    65341 aaaaaattcg cctgcttcat gcgcaaaggc actgccagaa agaagagttg ttactgccaa
    65401 agccgccact gttaactttt tcatatccgc tccgtcgtta tggttataaa aacgtgacga
    65461 atatacctac attggagtaa caaatgatcc tacatagatc acattacata tgataattca
    65521 ccatttattg atctggatta atttagaagt tgcattgaga atcctggtta atgtgattta
    65581 aatcaatttt taaatatttt ctctacgcaa atattaactc gatcgctact tgccgcggtt
    65641 tgcccacgcc tgaaggccag caattacctc cgctttacaa agatatgctt ttccatacaa
    65701 gacctgatgc tgccacccga ggcttaacca gggtacaatt gcccgctaat taacacctgc
    65761 ataaaactca aggagagtgc atgtctatca cggcgcagtc cgtataccgt gacaccggaa
    65821 atttcttccg taatcaattt atgaccattc tgttggtatc gttgctatgt gcgtttatca
    65881 cagtggtgtt agggcatgtt ttctcaccca gtgatgcaca gcttgcgcag ctcaatgacg
    65941 gcgtgcccgt tagcggcagt agtgggttgt tcgacctggt tcagaatatg tcaccggaac
    66001 agcaacaaat tttgctgcag gcttcagcgg cgtccacttt ttcagaatta atcggtaacg
    66061 ccattctcgc cggaggcgta atattaatta tccagctggt gtctgcgggt cagagagtca
    66121 gtgcgctgcg agctattggt gccagtgcgc cgatattgcc caagttattt attctgattt
    66181 ttctgactac ccttttagta cagattggca tcatgctggt ggtcgttccg ggaattatca
    66241 tggccatttt actggccctg gcaccggtga tgctggtaca ggacaaaatg ggcatttttg
    66301 cctcgatgcg tagcagtatg cggctgactt gggcgaatat gcgtctggtg gcacccgcag
    66361 tactgagctg gttgctggca aaaacactgt tgctgctttt tgcctcttct tttgccgcat
    66421 taaccccgga aattggtgcc gtactggcga acaccttgag caacctgatt tctgccatat
    66481 tgctcatcta tctgttccgc ctgtatatgt tgattcgcca ataaccttaa gcatttgatt
    66541 acggaatcgt aaaatgaagc agtttcttga ttttttaccg ctggttgtct ttttcgcgtt
    66601 ttacaagatt tatgacatct atgcggctac tgcagcgctg atcgtcgcca cggcgattgt
    66661 gcttatctat agctgggttc gctttcgtaa ggttgagaag atggccctga tcacttttgt
    66721 tctggtggtc gtcttcggtg gcttgacgct gttcttccac aatgatgagt ttattaaatg
    66781 gaaggttaca gtcatttatg ctctgtttgc gggagccctg ttagtcagcc aatgggtgat
    66841 gaaaaagccg ctaattcagc ggatgctggg taaagaactc acgctgccgc aaccggtatg
    66901 gtcgaagcta aatctggcct gggctgtttt ctttatcctt tgcggtctgg caaacatcta
    66961 catcgcattc tggctgccgc aaaatatttg ggtcaacttt aaagtctttg gcctgaccgc
    67021 ccttacctta atcttcacat tgttaagcgg tatctatatc taccgccaca tgccgcagga
    67081 agataaatcc taaccagact gccagtcctt cggggctggc gctgtatctc ctctgttctc
    67141 ctcaggaaaa tcatagtagc atcgcgcctg tgattttcct tttaagtcgg ttttaccatg
    67201 tctacaacac ataacgtccc tcagggcgat cttgttttac gtactttagc catgcccgcc
    67261 gataccaatg ccaatggtga catctttggt ggttggttaa tgtcacaaat ggatattggc
    67321 ggcgctattc tggcaaaaga aattgcccac ggtcgcgtag tgactgtgcg ggttgaagga
    67381 atgactttct tacggccggt tgcggtcggc gatgtggtgt gctgctatgc acgctgtgtc
    67441 cagaaaggga cgacatcggt cagcattaat attgaagtgt gggtgaaaaa agtagcgtct
    67501 gaaccaatcg ggcaacgcta taaagcgaca gaagcattat ttaagtatgt cgcggttgat
    67561 cctgaaggaa aacctcgcgc cttacctgtt gagtaatagt caaaagcctc cggtcggagg
    67621 cttttgactt tctgcttact gaatttcggt ggtgccgtta attttaaaca ggatattcac
    67681 cacaatccca ctgcctggct tacccggctc ataacgccat ctgcgcatcg catttttcac
    67741 ctcacgctca aacatgttcg caggcttggc tgagaggatt tgtacgttat ccacgcgacc
    67801 atccggcgtg acgtcaaatt taactttaac ctgcccttca atgcgcaatg cctgtgctcg
    67861 tgccggatac tgcggctgat tacggcttaa tgcgcgtggt cctgaagcca cactggtaac
    67921 cggcttgctg gttgcagccg ttgccgtact tgatgtgggg cgcgcgggtg ccgtattttc
    67981 aaacggtgat gccggacgcg actctacggg tttgacatcg cgttttggct gctcctgtac
    68041 ctttttcacc ggcttcggtt ttggcttagg tttcggcttc ggcttttcaa tgaccaccgg
    68101 tgcttctttt ggcggttcgg ggatcggctc aggttccggt tctggctcta ccaccggctc
    68161 cggtggcggc tgaacggctt gtggcggttc gagatcagca ggcgcaacca tcgtgacaga
    68221 aattggttgc gcaggcgcag gtagttcaat aacctgatgt accgaggtat agagcagacc
    68281 cgccacaaca gcaccatgaa tgcagaccga aagtaacgtc ggccagggga agcggcgagg
    68341 taaatcaagg gttattgaag tcataatcat ttcagtagaa aaaccaagcc tcgattttaa
    68401 atgcaaatag caatcatatt caataaggca agacgatcag gatcagtgaa aatgtcattt
    68461 ttggcgtaat tacccttact ttatgcatga ccttaacaaa gacatcatct ttccgttgca
    68521 gatcttcgcc ctttcaaata acgtacttta caactttccc gaacaaggag ttgtgcccgt
    68581 gttgtatgtt atttacgctc aagacaaagc tgatagcctc gaaaaacgcc tttccgttcg
    68641 tccggcacat ttagcacgtt tacagttact gcatgatgaa ggtcgtttgt tgacagctgg
    68701 tccaatgcca gcggtagaca gtaacgatcc aggggctgcg ggatttactg gctcaaccgt
    68761 tattgctgaa tttgaatcgc tggaagcagc tcaggcctgg gccgatgctg acccgtacgt
    68821 agcggcagga gtctatgaac acgtttcagt gaaaccattt aagaaagtgt tctgagtaga
    68881 gatccaatat ttaagggcac ggtttttgca accgtgttct tgtttggcct ttccttatag
    68941 ccggtcagtg cattcactac cacaagcaga taataacttt atctgtattt ctttatatga
    69001 tgtgagcatg ttcaggaaaa aagccctttc ctgacgcaga gttgctcact caaaaccttt
    69061 gattactgcg gataaagtca tctactattg tttaacacat tcacgcgctg catgtatgtt
    69121 cagaaaatgt gcacgctaag ataattaaac aaaattgtgg ttacagaggt atcagtgagt
    69181 cactgggcta cattcaaaca aacagcaaca aatttatggg tgaccctacg gcacgacatt
    69241 ctcgcgctgg ccgtcttttt aaatggattg cttattttta aaacaatcta tggtatgtcg
    69301 gtcaatttgc ttgatatttt ccatatcaaa gcattttcag agttggatct ctccttgctg
    69361 gcaaacgccc cactatttat gctcggcgtc tttcttgtcc tgaactccat tggcttactg
    69421 ttccgggcaa agctcgcatg ggcaatcagt atcattttgt tgttgatagc gctaatttac
    69481 accctgcatt tttatccctg gctgaaattt agtattggat tttgcatttt tacgctggtg
    69541 tttttgctga tactgcgcaa agacttctcc cacagtagcg ccgcagccgg gacaattttt
    69601 gcatttatta gtttcacgac attactgttt tactcaacct acggtgcgct ttatttaagc
    69661 gaaggtttta atccgcgaat agaaagtttg atgaccgcgt tctatttttc gatagaaacc
    69721 atgtcaaccg tcggctatgg agatattgtc cctgtttctg aatcagcacg attgttcact
    69781 atttcggtca ttatttccgg cattaccgtt tttgccacat ctatgacctc aatttttggt
    69841 ccgcttatcc gcgggggatt caacaaactt ttaaaaggaa acaatcatac aatgcatcgt
    69901 aaagatcatt ttattgtttg cggacattcg attctcgcca tcaatacgat cctgcaactg
    69961 aatcaacgcg gacaaaacgt aacggttatc agcaacttgc ctgaagatga tatcaagcaa
    70021 cttgagcaac gcttaggcga taacgctgat gttatccccg gtgacagtaa tgacagttca
    70081 gtattaaaga aagcgggaat cgatcgttgc cgggccattc tggcgctgag tgataacgat
    70141 gcagataacg cgtttgttgt actctcggca aaagatatga gcagtgatgt caaaacagtt
    70201 ctcgccgtca gtgatagcaa aaacctgaat aagattaaga tggtacatcc ggatatcatt
    70261 ctctcaccgc aactgtttgg cagcgaaatt ctggcgcgag ttttaaatgg tgaagagatt
    70321 aataatgata tgctcgtttc aatgttgttg aactcaggtc atggtatttt tagcgataac
    70381 gatgaacaag aaacgaaagc tgacagtaaa gaatcggcgc aaaaatagag tttaaagacg
    70441 gaagagtaaa cttattgggc accgcaataa tggtgcccag tacatttaga tattgtagat
    70501 cgcaaacaat aacgagttac gctgtttgtt tagaatgcac ttcctgatgc tgtggatacg
    70561 catatttcgg cgcgattgcc cctcaagcca acgcgattta cgtcggctag cctgacgctg
    70621 catgcgccag cgccccactt ccgttctact ccgcttcatg ttaactactc tatgcaataa
    70681 cagaatggtc attatacccc cgcccccgac gctgaccagt gcttttcccg cgtttttatt
    70741 tgccagatga atccatatgc gtaaactcat aacaatgcgc tttcaaaagg atttctaatc
    70801 tatgacaacc gtttatacgt tggtgagttg gttggccatt ctgggatact ggttgctcat
    70861 tgcaggcgta actttacgca ttctaatgaa acgacgcgca gttccctccg cgatggcctg
    70921 gctgttgatt atttacattc tgccgttagt cggaattatt gcctatcttg ccgttggcga
    70981 gctccattta ggcaaacgcc gcgctgagcg cgccagagcg atgtggcctt ccaccgcaaa
    71041 atggcttaac gaccttaaag cctgtaagca tatcttcgcc gaagaaaata gcagtgtcgc
    71101 tgcgccatta ttcaagcttt gcgagcgtcg tcaggggatc gctggggtca aagggaatca
    71161 gctacaactg atgaccgagt cagatgatgt gatgcaggcg ttaatccgcg acatccagct
    71221 cgcgcgccat aatattgaga tggtgtttta tatctggcag cccggcggca tggcggacca
    71281 ggtggctgaa tcattaatgg cggctgcacg gcgtggtatt cattgccgat tgatgctcga
    71341 ctccgccggg agtgtggctt ttttccgcag cccgtggccc gagctaatgc gtaatgccgg
    71401 tattgaagtg gtcgaagcct taaaggtcaa tctgatgcgt gtgtttttac gccgtatgga
    71461 cctgcgccaa catcgcaaga tgatcatgat cgataattac atcgcgtaca ccggcagcat
    71521 gaatatggtc gatcctcgct acttcaaaca agatgcgggc gtagggcaat ggattgatct
    71581 gatggcgcgt atggaaggcc ccatcgccac cgcgatgggg attatttatt cctgcgactg
    71641 ggagattgaa accggaaaac gtattctgcc gccaccacca gatgtcaata ttatgccgtt
    71701 tgaacaggcc agcggtcaca ccattcacac aattgcttct ggccccggct ttccggaaga
    71761 tctcattcac caggcattat tgactgcggc ttattcggcg cgtgaatatt tgatcatgac
    71821 cacgccctac tttgtgccaa gcgatgattt acttcatgcg atttgcacgg cggcgcagcg
    71881 cggggtggat gtcagtatta tccttccgcg aaaaaatgac tcgatgctgg tcggctgggc
    71941 cagtcgcgca ttctttacgg aactgcttgc tgctggggtt aaaatttatc agtttgaagg
    72001 cgggttactg cataccaaga gcgtgctggt cgatggcgaa ctaagtctgg ttggcaccgt
    72061 taaccttgat atgcgtagtc tgtggctgaa tttcgagatt accctggcaa tcgacgataa
    72121 aggttttggt gcagaccttg ccgccgttca ggacgattat atttcgcgtt cacgtctgct
    72181 cgatgcccgt ttatggctaa aacgtccatt atggcaacgt gtcgccgagc gactgtttta
    72241 cttcttcagc ccgttgctgt aaaacgtcgc caacagaggt taaacaggta gtgactatgg
    72301 atatggatct aaacaatcgc ctgactgaag atgaaacgct tgagcaggcc tacgatattt
    72361 ttctcgaact ggctgccgac aatctcgatc cagctgatgt tctgctgttc aatcttcagt
    72421 ttgaagagcg cggcggcgcg gagttattcg atccggcgga agactggcag gaacatgttg
    72481 atttcgattt gaacccggac ttttttgccg aagtggtgat tggtctggcg gacagtgaag
    72541 atggtgaaat caacgatgtt ttcgcccgca ttctgttatg ccgcgaaaaa gatcacaaac
    72601 tttgccatat catctggcgg gaataaataa tcgcttttag cgtgaaaaag ggctgacaac
    72661 tgtcagccct tattgttttt aaagcggatc gactttcagg caagaaacgg cgtgtctgaa
    72721 actcccctcc agaacaggac gtgttttggc gcactccgga ccggcaatcg ggcaacgggt
    72781 acggaaaaca caaccggaag gcgggttgat cggcgacggt aattcccctt ccagtaactg
    72841 gatggttttg ttcttctcca gatccggatc aggtatgggg actgccgaca tcaatgccct
    72901 ggtgtaagga tgtagtggat tgtggtagac ctcatcatag gtccccagtt ctactgcatg
    72961 gccgagatac atcaccaaca cacgatcgga aatgtgtttt accacggcca ggtcatgagc
    73021 aataaaaatt aatgacaatc ccatctcacg ttgcagctgc tggagcaggt tgaccacctg
    73081 cgcctgaatt gacacgtcca gcgccgaaac cggctcatcg cagataatca gcttcggttc
    73141 aagaataaga gcacgagcaa tcccgatacg ctggcactgc ccaccggaga actcatgcgg
    73201 atagcggtta atcaggttag gcaataaccc gactttcagc atcatcgcct tcacgcgctc
    73261 gcgaacttcc tggcgtgaca ttttcggatg ataagtacgc agtggttcag cgatgatctc
    73321 gccgatggtc atacgcgggt ttagcgatgc caacggctcc tggaaaatca tctgaatatc
    73381 actgcgaacg gcacgccatt catcgggctt catgcccagc aactctttac ctaaccaggc
    73441 aacatgaccg tcggtcgcct tgaccaaacc gatgatggcg cgagcaaagg tggacttacc
    73501 gcatcccgat tcccctacca cacctaatgt ttccccttca tacaggcgaa gagtgacacc
    73561 atcgacggct ttgagcgttt tcggcggttg ccagaaccac tgtttgccat ctttgatttc
    73621 aaagtgcact ttaagatcgg cgatttcaag gaggactttt cttccttcag ttacagcatt
    73681 cataacagtt cctccaccgg tttaaagcaa gcacgcagac ggccaggcgt aaactcttcc
    73741 agcggcggtg cgctactaca aatttccatc gcatgtggac aacgtggctg gaacgggcaa
    73801 cctttcggta atcgcagcaa gtttggcgga ttacccggga tggtcaacat tgtttcacct
    73861 tccgcatcaa gacgcggcac cgcgttgagc aaaccgatag aataaggatg aacgggttga
    73921 taaaagacat cgcgcgcatt gccatattcc atcgtgcgcc cggcgtacat taccagcact
    73981 ttgtcacaga tccccgccac cacgccaaga tcgtgggtga tcataatgat ggcagtatta
    74041 aattcccgct tcagttcatt caatagcgtc atgatctgcg cctgtacggt gacgtccagc
    74101 gctgtagtgg gttcatccgc aatcagcaac ttaggtcgac atagcaatgc catcgcaatc
    74161 atgactcgct gacgcatgcc gccagaaaat tcgtgcgggt acattttcat gcgtttacgt
    74221 gcttccggca tttttaccgc atcgagcatc cgcaccgact cttcaaacgc ctcagctttg
    74281 ctcatgttct tatgcagcat cagcacttcc atcaactgct caccgacgcg catataggga
    74341 ttcaacgaag tcattgggtc ctgaaaaatc attgagattt gttcagcgcg cagtttattg
    74401 agttcacgct ctggcaaatt gaggatttca cgcccattga aggttgccgc tccgccaatg
    74461 cgtccgttgg cagccaacag gcccatcaac gcaaatgcag tttgcgattt acccgaacca
    74521 gactcaccta caatgcccag cgtttctccg gcacgtaggg aaaaatttaa atcattgacc
    74581 gccgtgacgt cgccgtccgg ggtactaaag gtgacacgca aatctttcac gttcagcagt
    74641 gcgtcagcct gttgttgtgc gagcggcaca gttgcagttt caattacgct catggctgca
    74701 ctccttaacg atctttcggg tcgagggcat cacgcaagcc atcgccgata aagttgaaac
    74761 aaaacagtgt caccacaagg aatcccgctg ggaacaacag taaccatgga gagacttcca
    74821 tcgagttcgc gccatcactc agcaatgccc cccagctgct taacggctct tgcgtaccca
    74881 accccaggaa gctaaggaaa gattcaaaga ggatcatgct gggcaccagt agcgatgcgt
    74941 agaccaccac cacaccgagt acgttcggca caatgtggcg aataacaatg cccggcgtcg
    75001 atacaccgcc aacttgtgcc gcctcaataa actctttgcg cttcagactc agggtttgcc
    75061 cacgcacaat acgagccata tccagccagg aaaccatgcc aatcgccacg aaaatcagca
    75121 ggatgttttg gccgaaaaag gtcaccagca aaatgacgaa gaacatgaat gggaaggagt
    75181 tgaggatttc cagcagacgc atcattaccg aatccacttt accgcccaga taaccggaaa
    75241 gcgaaccata aagtgtcccc acgaccactg ccaccagtgc cgcagcaaca ccgaccatga
    75301 gtgagatacg cccgctaatc gcaacgcgca caagcaggtc gcgaccggat gagtcagtac
    75361 caaagtagtg accggactcc atatccgggg cgctggacat catcgcccag tcagtatcgt
    75421 cataggcaaa ctgggaaagc atcggtgcca ggattacaaa taacgcgatc agcaccagca
    75481 ctatcagact ggcaaccgcc gcacggttat gcataaaacg tcgacgtgcg tcctgccaca
    75541 agctgcgccc ttcgacctcc agcttttcac tgaaattttc cagcgtctcg ctgtttttct
    75601 tacttaacat catcgcgagc tccagtatca gtaacggatt ttcgggtcga taaccgcata
    75661 tagcacatcg acaatggcat taaacaaaat ggttaacgca ccaaccagga tggtcaggct
    75721 taacactaag gaatagtcac ggttcaatgc gccattaacg aacagttgcc caatccccgg
    75781 caaaccataa atggtttcga tgaccataga accggtaata atgccgacaa atgcggggcc
    75841 catataggag agcacgggta acagagcagg ttttaatgcg tggcgtaaaa tgatccgccg
    75901 cataggtaac cctttcgccc gggcagtacg aataaagttg gagtgtaata cttcaatcat
    75961 agagccacgg gtaatacgcg caatactggc gatataggcg agtgacaacg ccaccatcgg
    76021 caatatcatg aatttaagcg ccccaccatt ccagccaccg cccggcagcc aatgcaaaat
    76081 gatcgcaaat atcatgacta ataatggcgc aaccacaaaa ctggggataa caaccccggt
    76141 cattgccagc cccatcacgg tatagtccca tttggtgttt tgttttaatg cggcaataac
    76201 gccagcacta acacccagta ttaccgcaag gaaaaatgcg gcggctccca gtttggcaga
    76261 aacgggaaaa ctggatgcca ccaggtcatt gaccgaataa tctttatatt taaacgatgg
    76321 accgaaatcg ccatgcgcca gttgtttcag gtagctgaaa tactgtgtca tgatcggatc
    76381 attaagatga tatttcgctt cgatattggc catcacttct ggcggtaaag tacgttcgcc
    76441 ggtaaaaggg cttcccggcg cgaggcgcat cataaagaac gaaatagtaa taagaataaa
    76501 tagcgtcgga atcgcttcca gacagcgacg tagaataaat tttaacattg ccctaccttc
    76561 tggcctgtgc cttctgtaat gcgataaaaa tcagacaccg tggagcagga cactcctgcc
    76621 ccacgtattg ccattagtgc ttcacaatgt acatattccg ggtataggta ttatccagcg
    76681 gatctttgcc ggtatagcca ccaacccacg gtttcaccag acgcgcattc acgtagtaat
    76741 aaacaggaac aatggccgaa tccttatcca gctgttgttc tgctttagtg tacagagctg
    76801 tgcgctgcgc ctcgtcagtc actttcagcg tttccgccat aatgctgtca aaggccgggc
    76861 tcttataatg cgcggtattc atcgagctgt tcgacagcat ggtgttcagg aaggaagttg
    76921 gttcgttgta gtctgcacac cagcctgcac gggccacatc aaaagtgccc tggtgacggg
    76981 tgtcgaggaa cgttttccac tcctggttaa ccagtttgac gtttacacca atgtttttct
    77041 tccacaatga agaggcagca atcgccagct ttttatgcag atcggaggtg ttataccgca
    77101 ggttgatggt caacggtttg tctgcggtat aacccgcttc agccagcagt tttttcgctt
    77161 cttcgttacg tttttcctgg ctccagccaa accattctgg ctgagtcaat tttgcgccag
    77221 cagtatacgg tggagtgtaa ccataggcgg gcatgtcgcc ctgcgctttc actttattaa
    77281 caatgatatc gcggtccata cctagtttca gtgcggtacg cacacgcaca tcgttgaatg
    77341 gcggtttctg gttgttaatt tcgtaatagt aagtgcacag gtatggatca acgtgaactt
    77401 cgtccgggat ctcttttttc agcttctgga acaattcgat cggcatgttg ttatacgtca
    77461 tgtcgatttc accactacgg tagcggttga catcggtaac ttcagaagca ataggcaaat
    77521 aggttacctg gttaataacg gttttcgcgt tgttccagta ggtcgggctg cgttcaagaa
    77581 cgattcgttc gtttacgacc caatctttta aggtataggc accgttggtg acgatattac
    77641 caggctgggt ccatttttcg ccgaatttct cgatagcggc ttttggcacc ggtgaagttg
    77701 atgggtgaac aagtaattta tagaagtacg gaacgggttc acttaaggtg acttctaatg
    77761 tgtgatcatc aatagctttc acgccgagat cggtaatcgg ttttttccct tcaagaattt
    77821 catcaatacc ggcgatatgc ccatattgca gataactggc atacggagaa gcagtgttcg
    77881 gatcaacaga acgttgccag ctatacacaa agtcttgtgc tgtgactggc gtgccatcag
    77941 accatttcgc atctttacgc aaatggaagg tccagacttt cgcgtcttta ttatcccagg
    78001 attcagcgac gccaggtgct ggatgaccgt caagatcgct gaccagtaag ccttcaaaca
    78061 ggtctcggct gatattagac tccggaacac cttcaatttt gtgcggatct aatgactgaa
    78121 cttctgaacc attgttacgt accagtgttt gtttttccgc cagtgtgacg cctgcgggta
    78181 catcagctgc cagcgcgaca ttccctgcca ttagcgcagc cagaacgcca gctgctacta
    78241 aacttctctt ggtgatgttg gtcattgttt tttggactcc ctcattataa ttactggtat
    78301 taaccagcat gtgtaatccc ggattgggac cctgcacagg ctcggagact ttctgctgtc
    78361 aggttacttt atcgttacgt attactatca ccgactttat ttattctggt gattcagacg
    78421 atcaccttat gtcgattcgt actgtgtctc ccctgtcgag acacattatt ctttcgcgac
    78481 gctctttaat tgttaattat tctcattacc aataaagaat cgtctggcgg ttgcccggaa
    78541 agtaccaaat gcggttctta ctcgccaata cattttgcaa aattgttaag cgattctctt
    78601 ttattgtccg tatcagaatc tatagcttgc atccatgcct taacaaacat ctttcaattc
    78661 aatcaaatag agattggcta agcatgtcta tcttttctga ttacagttcc agtagtgaaa
    78721 tgcacaataa tttaacaatt gattattatt tagcactttc ctctaccaaa ggcagtggaa
    78781 ttactaatat catttcaatt attcttcagc aagcccagga ttatgatgtg gcgaaaataa
    78841 catgatggtg aggagaagca gttaaggaaa tccttataac taactgtaat ttgagaagaa
    78901 tatttttacc tattcctttt gcttttgata actgcgcggg aacaaagtaa aaataatgac
    78961 gaagtaaacg gagcatggca gctccgtttc atttgaaagg aattaattaa gcaggccggg
    79021 gaaaatcccc ttaataccag taacgataaa ttcaatcccc aatgccatca gcaatagccc
    79081 cataatacgc gtaatcacgt tgatgccggt ctggcgtaaa acccgtacca gccacggtgc
    79141 catgcggaac aatccccagc aacataaagc gaacaatgca atagccacaa agaaaccaaa
    79201 cagatagcta atgctgtgat aacgcgtacc ccagacgatg gtagaactga tcgcccctgg
    79261 ccccgccatc aacggcaacg ccaatggcac cacaccaata ctttcacgta ccgcggtttc
    79321 tgatttttct tgcttgttct gtttatcctc gccaagcttg ccgctgatca tcgacatcgc
    79381 tatcgtcacc accaggatgc ccccggcgat acggaacgaa tcaattgata taccaaaaag
    79441 ttgtagaatc gtgtcgccga gaaaaagcga gatccacaag ataatggcca cagacaggtt
    79501 ggctgtaagg ttagttttgt ttcgcgctgc cgctgtctga taactggtca tgctgataaa
    79561 gacgggaata atccctaccg ggttgaccag cgcaaataac ccgatgaaaa atttgaagta
    79621 aacgggaaaa tcaaaaaggg tctgaatcac ggttagctcc gaagcaaaag ccggataatg
    79681 ttagccataa ataaggttga aaagacgcgc tgacaatacg ccttttgaca gcatttttca
    79741 cctcctaact acttaaaatt gctatcattc gttattgtta tctagttgtg caaaacatgc
    79801 taatgtagcc accaaatcat actacaattt attaactgtt agctataatg gcgaaaagcg
    79861 atgctgaaag gtatcagctt tgcaaaaatt tgatttggat cacgtaatca gtacccagaa
    79921 gtgagtaatc ttgcttacgc cacctggaag tgacgcatta gagataataa ctctaatgtt
    79981 taaactcttt tagtaaatca cagtgagtgt gagcgcgagt aagcttttga ttttcatcgg
    80041 ttaagcaaat catcaccgca ctgactatac tctcgtattc gagcagatga tttactaaaa
    80101 aagtttaaca ttatcaggag agcattatgg ctgttactaa tgtcgctgaa cttaacgcac
    80161 tcgtagagcg tgtaaaaaaa gcccagcgtg aatatgccag tttcactcaa gagcaagtag
    80221 acaaaatctt ccgcgccgcc gctctggctg ctgcagatgc tcgaatccca ctcgcgaaaa
    80281 tggccgttgc cgaatccggc atgggtatcg tcgaagataa agtgatcaaa aaccactttg
    80341 cttctgaata tatctacaac gcctataaag atgaaaaaac ctgtggtgtt ctgtctgaag
    80401 acgacacttt tggtaccatc actatcgctg aaccaatcgg tattatttgc ggtatcgttc
    80461 cgaccactaa cccgacttca actgctatct tcaaatcgct gatcagcctg aagacccgta
    80521 acgccattat cttctccccg cacccgcgtg caaaagatgc aaccaacaaa gcggctgata
    80581 tcgttctgca ggctgctatc gctgccggtg ctccgaaaga tctgatcggc tggatcgatc
    80641 aaccttctgt tgaactgtct aacgcactga tgcaccaccc agacatcaac ctgatcctcg
    80701 cgactggtgg tccgggcatg gttaaagccg catacagctc cggtaaacca gctatcggtg
    80761 taggcgcggg taacactcca gttgttattg atgaaactgc tgatatcaaa cgtgcagttg
    80821 catctgtact gatgtccaaa accttcgaca acggcgtaat ctgtgcttct gaacagtctg
    80881 ttgttgttgt tgactctgtt tatgacgcag tacgtgaacg tttcgcaacc cacggcggct
    80941 atctgttgca gggtaaagag ctgaaagctg ttcaggatgt tatcctgaaa aatggtgcgc
    81001 tgaacgcggc tatcgttggt cagccagcct ataaaattgc tgaactggca ggcttctctg
    81061 taccagaaaa caccaagatt ctgatcggtg aagtgaccgt tgttgatgaa agcgaaccgt
    81121 tcgcacatga aaaactgtcc ccgactctgg caatgtaccg cgctaaagat ttcgaagacg
    81181 cggtagaaaa agcagagaaa ctggttgcta tgggcggtat cggtcatacc tcttgcctgt
    81241 acactgacca ggataaccaa ccggctcgcg tttcttactt cggtcagaaa atgaaaacag
    81301 ctcgtatcct gattaacacc ccggcttctc agggtggtat cggtgacctg tataacttca
    81361 aactcgcacc ttccctgact ctgggttgtg gttcctgggg tggtaactcc atctctgaaa
    81421 acgttggtcc gaaacacctg atcaacaaga aaaccgttgc taagcgagct gaaaacatgt
    81481 tgtggcacaa acttccgaaa tctatctact tccgccgtgg ctccctgcca atcgcgctgg
    81541 atgaagtgat tactgatggc cacaaacgtg cgctcatcgt gactgaccgc ttcctgttca
    81601 acaatggtta tgctgatcag atcacttccg tactgaaagc agcaggcgtt gaaactgaag
    81661 tcttcttcga agtagaagcg gacccgaccc tgagcatcgt tcgtaaaggt gcagaactgg
    81721 caaactcctt caaaccagac gtgattatcg cgctgggtgg tggttccccg atggacgccg
    81781 cgaagatcat gtgggttatg tacgaacatc cggaaactca cttcgaagag ctggcgctgc
    81841 gctttatgga tatccgtaaa cgtatctaca agttcccgaa aatgggcgtg aaagcgaaaa
    81901 tgatcgctgt caccaccact tctggtacag gttctgaagt cactccgttt gcggttgtaa
    81961 ctgacgacgc tactggtcag aaatacccgc tggcggacta tgcgctgacc ccggatatgg
    82021 cgattgtcga cgccaacctg gttatggaca tgccgaagtc cctgtgtgct ttcggtggtc
    82081 tggacgcagt aactcacgcc atggaagctt atgtttctgt actggcatct gagttctctg
    82141 atggtcaggc tctgcaggca ctgaaactgc tgaaagaata tctgccagcg tcctaccacg
    82201 aagggtctaa aaatccggta gcgcgtgaac gtgttcacag tgcagcaact atcgcgggta
    82261 tcgcatttgc gaacgccttc ctgggtgtat gtcactcaat ggcgcacaaa ctgggttccc
    82321 agttccatat tccgcacggt ctggcaaacg ccctgctgat ttgtaacgtt attcgctaca
    82381 atgcgaacga caacccgacc aagcagactg cattcagcca gtacgaccgt ccgcaggctc
    82441 gccgtcgtta tgctgaaatt gccgaccacc tgggtctgag cgcaccgggc gaccgtactg
    82501 ctgctaagat cgagaaactg ctggcatggc tggaaacgct gaaagctgaa ctgggtattc
    82561 cgaaatctat ccgtgaagct ggcgttcagg aagcagactt cctggcgaac gtggataaac
    82621 tgtctgaaga tgcattcgat gaccagtgca ccggcgctaa cccgcgttac ccgctgatct
    82681 ccgagctgaa acagattctg ctggatacct actacggtcg tgattatgta gaaggtgaaa
    82741 ctgcagcgaa aaaagaagcc gctccggcta aagctgagaa aaaagcggaa aaatccgctt
    82801 aatcagtagc gctgtctggc aatataaacg gccccttc
//
LOCUS       DADLSA010000004        64963 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.004, whole genome shotgun sequence.
ACCESSION   DADLSA010000004 DADLSA010000000
VERSION     DADLSA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.004"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="JN691_000274"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="JN691_000274"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAY7241811.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JN691_000275"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JN691_000275"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAY7241812.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JN691_000276"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JN691_000276"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAY7241813.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="JN691_000277"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="JN691_000277"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAY7241814.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="JN691_000278"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="JN691_000278"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAY7241815.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDTLGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCCDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /gene="cptA"
                     /locus_tag="JN691_000279"
     CDS             7826..9559
                     /gene="cptA"
                     /locus_tag="JN691_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAY7241816.1"
                     /translation="MHSTEVQAKPLFSWKTLGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQAMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="JN691_000280"
     CDS             9708..10559
                     /locus_tag="JN691_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7241817.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="JN691_000281"
     CDS             complement(10546..10887)
                     /locus_tag="JN691_000281"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY7241818.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="JN691_000282"
     CDS             complement(10889..11767)
                     /locus_tag="JN691_000282"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAY7241819.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="JN691_000283"
     CDS             complement(11733..14030)
                     /locus_tag="JN691_000283"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY7241820.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNLRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="JN691_000284"
     CDS             complement(14081..14401)
                     /locus_tag="JN691_000284"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY7241821.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15480)
                     /locus_tag="JN691_000285"
     CDS             complement(14416..15480)
                     /locus_tag="JN691_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAY7241822.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAL
                     IAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGALTNSL
                     TQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAIAAVGI
                     CIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVIPSIMV
                     GSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVLKSLAR
                     KNGSSTDEKEDDLDLDFEIN"
     gene            15789..18290
                     /gene="ptsP"
                     /locus_tag="JN691_000286"
     CDS             15789..18290
                     /gene="ptsP"
                     /locus_tag="JN691_000286"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7241823.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSL
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18302..18964
                     /gene="fsa"
                     /locus_tag="JN691_000287"
     CDS             18302..18964
                     /gene="fsa"
                     /locus_tag="JN691_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY7241824.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVITNPSIIAASKESIWEALP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAEGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            18975..19754
                     /locus_tag="JN691_000288"
     CDS             18975..19754
                     /locus_tag="JN691_000288"
                     /inference="COORDINATES: protein motif:HMM:NF006941.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HAY7241825.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEITPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTHAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGWRRLTRCITA"
     gene            19739..20022
                     /locus_tag="JN691_000289"
                     /pseudo
     CDS             19739..20022
                     /locus_tag="JN691_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005111035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
     gene            20411..20620
                     /locus_tag="JN691_000290"
     CDS             20411..20620
                     /locus_tag="JN691_000290"
                     /inference="COORDINATES: protein motif:HMM:NF018626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAY7241826.1"
                     /translation="MADGARQQIGSTLFYDPAYIQLTYPGGDVPQERGVCSDVVIRAL
                     RSQKVDLQKLVHEDMAKNFAEYPQK"
     gene            20660..20914
                     /locus_tag="JN691_000291"
     CDS             20660..20914
                     /locus_tag="JN691_000291"
                     /inference="COORDINATES: protein motif:HMM:NF018626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAY7241827.1"
                     /translation="MPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLAHIGVV
                     SDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(20941..21879)
                     /gene="yijE"
                     /locus_tag="JN691_000292"
     CDS             complement(20941..21879)
                     /gene="yijE"
                     /locus_tag="JN691_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAY7241828.1"
                     /translation="MSTGKLHTGSLMSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVT
                     SYIGAFDFTALRCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWAL
                     VSGGAGKVAILSYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFS
                     SMKSAMLAILSGVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQ
                     REIDWQPTVFWALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWL
                     LGENPGAVEGSGIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21940..24120)
                     /gene="katG"
                     /locus_tag="JN691_000293"
     CDS             complement(21940..24120)
                     /gene="katG"
                     /locus_tag="JN691_000293"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAY7241829.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24449..25339)
                     /gene="metF"
                     /locus_tag="JN691_000294"
     CDS             complement(24449..25339)
                     /gene="metF"
                     /locus_tag="JN691_000294"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAY7241830.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRSEMSYAICHTLGVRPGL"
     gene            complement(25688..28120)
                     /locus_tag="JN691_000295"
     CDS             complement(25688..28120)
                     /locus_tag="JN691_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAY7241831.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28123..29283)
                     /gene="metB"
                     /locus_tag="JN691_000296"
     CDS             complement(28123..29283)
                     /gene="metB"
                     /locus_tag="JN691_000296"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAY7241832.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEHALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29560..29877
                     /gene="metJ"
                     /locus_tag="JN691_000297"
     CDS             29560..29877
                     /gene="metJ"
                     /locus_tag="JN691_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAY7241833.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30061..30669
                     /locus_tag="JN691_000298"
     CDS             30061..30669
                     /locus_tag="JN691_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAY7241834.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30730..30942)
                     /gene="rpmE"
                     /locus_tag="JN691_000299"
     CDS             complement(30730..30942)
                     /gene="rpmE"
                     /locus_tag="JN691_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAY7241835.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31145..33343
                     /gene="priA"
                     /locus_tag="JN691_000300"
     CDS             31145..33343
                     /gene="priA"
                     /locus_tag="JN691_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAY7241836.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLHLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33499..34524
                     /gene="cytR"
                     /locus_tag="JN691_000301"
     CDS             33499..34524
                     /gene="cytR"
                     /locus_tag="JN691_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAY7241837.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALCRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34616..35575
                     /gene="ftsN"
                     /locus_tag="JN691_000302"
     CDS             34616..35575
                     /gene="ftsN"
                     /locus_tag="JN691_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAY7241838.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADTPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35668..36198
                     /gene="hslV"
                     /locus_tag="JN691_000303"
     CDS             35668..36198
                     /gene="hslV"
                     /locus_tag="JN691_000303"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAY7241839.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36208..37539
                     /gene="hslU"
                     /locus_tag="JN691_000304"
     CDS             36208..37539
                     /gene="hslU"
                     /locus_tag="JN691_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAY7241840.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLLPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKMKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37606..38532
                     /gene="menA"
                     /locus_tag="JN691_000305"
     CDS             37606..38532
                     /gene="menA"
                     /locus_tag="JN691_000305"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAY7241841.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38625..39110
                     /gene="rraA"
                     /locus_tag="JN691_000306"
     CDS             38625..39110
                     /gene="rraA"
                     /locus_tag="JN691_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAY7241842.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRILVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39195..39440)
                     /gene="zapB"
                     /locus_tag="JN691_000307"
     CDS             complement(39195..39440)
                     /gene="zapB"
                     /locus_tag="JN691_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAY7241843.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39866..40711
                     /gene="glpF"
                     /locus_tag="JN691_000308"
     CDS             39866..40711
                     /gene="glpF"
                     /locus_tag="JN691_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HAY7241844.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPFDICVVEEKETTTPSE
                     QKASL"
     gene            40734..42242
                     /gene="glpK"
                     /locus_tag="JN691_000309"
     CDS             40734..42242
                     /gene="glpK"
                     /locus_tag="JN691_000309"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAY7241845.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKTVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42472..43482
                     /gene="glpX"
                     /locus_tag="JN691_000310"
     CDS             42472..43482
                     /gene="glpX"
                     /locus_tag="JN691_000310"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY7241846.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43579..44325
                     /gene="fpr"
                     /locus_tag="JN691_000311"
     CDS             43579..44325
                     /gene="fpr"
                     /locus_tag="JN691_000311"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAY7241847.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44330..44758)
                     /gene="uspD"
                     /locus_tag="JN691_000312"
     CDS             complement(44330..44758)
                     /gene="uspD"
                     /locus_tag="JN691_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAY7241848.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44785..45084)
                     /locus_tag="JN691_000313"
     CDS             complement(44785..45084)
                     /locus_tag="JN691_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAY7241849.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45296..45736)
                     /locus_tag="JN691_000314"
     CDS             complement(45296..45736)
                     /locus_tag="JN691_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY7241850.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            45837..46436
                     /locus_tag="JN691_000315"
     CDS             45837..46436
                     /locus_tag="JN691_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAY7241851.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46544..47311
                     /gene="tpiA"
                     /locus_tag="JN691_000316"
     CDS             46544..47311
                     /gene="tpiA"
                     /locus_tag="JN691_000316"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAY7241852.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47366..48121)
                     /gene="cdh"
                     /locus_tag="JN691_000317"
     CDS             complement(47366..48121)
                     /gene="cdh"
                     /locus_tag="JN691_000317"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAY7241853.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48228..49217)
                     /locus_tag="JN691_000318"
     CDS             complement(48228..49217)
                     /locus_tag="JN691_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7241854.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49537..50499)
                     /gene="pfkA"
                     /locus_tag="JN691_000319"
     CDS             complement(49537..50499)
                     /gene="pfkA"
                     /locus_tag="JN691_000319"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAY7241855.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50680..51582)
                     /gene="fieF"
                     /locus_tag="JN691_000320"
     CDS             complement(50680..51582)
                     /gene="fieF"
                     /locus_tag="JN691_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAY7241856.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKILAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51731..52231)
                     /gene="cpxP"
                     /locus_tag="JN691_000321"
     CDS             complement(51731..52231)
                     /gene="cpxP"
                     /locus_tag="JN691_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAY7241857.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHKQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52381..53079
                     /gene="cpxR"
                     /locus_tag="JN691_000322"
     CDS             52381..53079
                     /gene="cpxR"
                     /locus_tag="JN691_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAY7241858.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53076..54449
                     /gene="cpxA"
                     /locus_tag="JN691_000323"
     CDS             53076..54449
                     /gene="cpxA"
                     /locus_tag="JN691_000323"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAY7241859.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54555..55229)
                     /gene="yiiM"
                     /locus_tag="JN691_000324"
     CDS             complement(54555..55229)
                     /gene="yiiM"
                     /locus_tag="JN691_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAY7241860.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIALGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(55378..56361)
                     /gene="kdgT"
                     /locus_tag="JN691_000325"
     CDS             complement(55378..56361)
                     /gene="kdgT"
                     /locus_tag="JN691_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAY7241861.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(56621..57241)
                     /gene="sodA"
                     /locus_tag="JN691_000326"
     CDS             complement(56621..57241)
                     /gene="sodA"
                     /locus_tag="JN691_000326"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAY7241862.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            57526..58560
                     /gene="rhaT"
                     /locus_tag="JN691_000327"
                     /pseudo
     CDS             57526..58560
                     /gene="rhaT"
                     /locus_tag="JN691_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(58557..59495)
                     /gene="rhaR"
                     /locus_tag="JN691_000328"
     CDS             complement(58557..59495)
                     /gene="rhaR"
                     /locus_tag="JN691_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HAY7241863.1"
                     /translation="MAFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFA
                     SDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADD
                     KHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVI
                     GQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAAS
                     LKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISD
                     ISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     gene            complement(59479..60315)
                     /gene="rhaS"
                     /locus_tag="JN691_000329"
     CDS             complement(59479..60315)
                     /gene="rhaS"
                     /locus_tag="JN691_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HAY7241864.1"
                     /translation="MTVLHSVDFFPSCNASVAIELRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            60603..62072
                     /gene="rhaB"
                     /locus_tag="JN691_000330"
     CDS             60603..62072
                     /gene="rhaB"
                     /locus_tag="JN691_000330"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAY7241865.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            62069..63328
                     /gene="rhaA"
                     /locus_tag="JN691_000331"
     CDS             62069..63328
                     /gene="rhaA"
                     /locus_tag="JN691_000331"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAY7241866.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            63570..64394
                     /gene="rhaD"
                     /locus_tag="JN691_000332"
     CDS             63570..64394
                     /gene="rhaD"
                     /locus_tag="JN691_000332"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAY7241867.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFAVTPLASALAL"
     gene            64404..64718
                     /gene="rhaM"
                     /locus_tag="JN691_000333"
     CDS             64404..64718
                     /gene="rhaM"
                     /locus_tag="JN691_000333"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAY7241868.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT        15822 a        17513 c        16630 g        14998 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcggga cactccagcg
     4081 atccagcacg cggagttaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgctgcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg tactgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatacgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acacaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgctgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttt
     7861 tagctggaaa accctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcaggc
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaaa tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cgctcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acgcccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc aggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccatga tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagctg ttaccaaccc aggcaccgat agcgcacgga gccagcgcag aacgctctgc
    15241 aatggagtaa ccaatgtaag cggcgaggaa aggcaccatc agcgtcaaac ctgcaacgcc
    15301 gatatcaaac agttttttca aattcggatc ggctacggca tccggcactg cgcctttgcc
    15361 atacaacatg acggaaaccg ccagcaaaat accgcccgat accacgaagg gaatcatgtg
    15421 tgaaacgccc gtcattaaat gctgacgggt atttttcagg atctgcacca actcattcat
    15481 aagctgctcc cgggcttgca aaagcccatg tccatatttt tgatggagga aacaataggc
    15541 aatcgcaggc ggagcaaaca gtggataaaa aggccattta ctggactttt gtaatatcag
    15601 tacaaaaatg cgatccgcct cataacttgc gataatccgg aagcatgccg caaaacggcg
    15661 tcttgtgaga cgcgtcgcaa attgacggtt acattacatt tgtccagctt ttggcagatt
    15721 tgtcacatgg caggccgtta ccgtgcgctc ttatcctgta aacagaaaac cgtaccggga
    15781 gaaaggcaat ggccctgatt gtggaattta tttgtgagct acctaacggc gtacatgcgc
    15841 gtccggcaag ccacgttgaa acgctgtgta atactttttc atctcaaatt gagtggcata
    15901 acctgcgtac tgaccgtaaa ggcaacgcca aaagcgctct ggcgctgatt ggcaccgata
    15961 cgctggtggg tgataactgc cagctactga tttccggggc cgacgaacag gaagggcacc
    16021 agcgtttaag ccaatggctg cgcgatgaat tcccccactg cgatgcgccg ctggcggaag
    16081 ttaaatctga cgaactggaa ccactgccgg tttcactgac caatctgaat ccgcaaatta
    16141 tccgcgcccg caccgtgtgc agcggtagtg caggcggcat tctgacgccg atctcttctt
    16201 tagatctcaa tgcgctgagt aatcttcccg cagccaaaga cgttgacgcc gagcaatccg
    16261 cactggaaaa cggcctgacg ctggtactga aaaacattga gtttcgtctg ctggatagcg
    16321 acggtgctac cagcgcgatt ctggaagcgc accgatccct ggctggcgat acttccctgc
    16381 acgaacattt actggcaggc gtcagcgccg gattaagctg cgccgaagcg attgtcgcca
    16441 gtgcgaatca cttttgcgaa gagttcgccc gttccagcag cagatacctg caagaacgtg
    16501 ccctagacgt acgcgacgtc tgcttccagt tactccagca aatctacggt gagcaacgct
    16561 tcccggcacc gggcaaactg acgcagtccg ccatttgtat ggctgatgaa ctgaccccca
    16621 gccagttcct cgaactggat aaaaatcacc tcaaaggatt gttactgaaa agcggcggca
    16681 ccacctcaca tacggtgatc cttgcccgtt cgttcaacat tccgacgctg gttggtgtgg
    16741 atattgatgc cctcactccg tggcagcatc aaacgattta tatcgacggc aacgccgggg
    16801 cgattgtggt tgagccagga gaagccgtag ctcgttatta tcagcaagaa gcccgcgtac
    16861 aggacgccct gcgtgagcaa cagcgtgtct ggctgaccca acaagcccgt accgctgacg
    16921 gtatccgcat tgaaattgcc gctaacatcg ctcactccgt ggaagcgcag gccgcattcg
    16981 gcaatggtgc ggaaggcgtt ggtttgttcc gcactgaaat gctctatatg gatcgcacca
    17041 gtgcaccggg cgaaagcgag ctgtacaaca ttttttgtca ggcgctggag tctgccaacg
    17101 gacgcagcat tattgtgcgc actatggaca ttggcggcga caaacccgtt gattatctaa
    17161 acattcccgc agaggcaaac ccgttcctcg gttatcgcgc cgtgcgtatt tatgaagaat
    17221 acgcgtcgtt atttaccaca caactacgct cgatcctgcg cgcctccgct cacggcagcc
    17281 tgaaaatcat gatcccgatg atctcctcac tggaagagat cttatgggtg aaagaaaaac
    17341 tggcagaagc caaacagcaa ctacgtaacg aacacattcc gtttgatgag aagatccagc
    17401 tcggcattat gctggaagtg ccgtcggtga tgttcatcat cgatcaatgc tgcgaagaga
    17461 ttgatttctt tagtattggt agtaatgacc tgacgcaata tctgctggca gtggatcgcg
    17521 ataacgctaa ggttactcgt cactacaaca gcctgaatcc ggcattcttg cgggcgctcg
    17581 attacgccgt gcaggcggtg catcgccagg gtaaatggat tggtctgtgc ggtgagctgg
    17641 gagcgaaagg ttccgtgctg ccgttgctgg tcggcttagg gctggatgaa ctcagcatga
    17701 gcgcaccatc aattccggcg gcgaaagcgc ggatggcgca acttgatagc cgtgagtgcc
    17761 gcaagttgct caaccaggca atggcctgcc gtacttcgct ggaagtggaa cacctgctgg
    17821 cgcaattccg catgacccaa caggacgcac cgctggtcac cgccgagtgc atcacgctgg
    17881 aaagtgactg gcgcagcaaa gaagaagtgc tcaaaggcat gaccgataac ctgctgctgg
    17941 cgggccgctg ccgctatccg cgtaaactgg aagccgactt atgggcgcgc gaggccgttt
    18001 tctctaccgg tctgggcttt agttttgcca ttccacacag caaatcagaa cacattgagc
    18061 aatccaccat cagcgtggcg cgtctgcaag cgccggtgcg ctggggcgat gatgaagcgc
    18121 aattcatcat tatgttaacc ctgaacaaac acgctgcggg cgatcagcac atgcgcattt
    18181 tctcgcgcct cgctcgccgc atcatgcacg aagaattccg taacgcgctg gttaacgccg
    18241 cctctgccga cgctatcgcc agcctgctgc aacatgaact ggaactgtaa aaggaaacat
    18301 catggaactg tatctggaca ccgctaacgt cgcagaagtc gaacgtctgg cacgcatatt
    18361 cccgattgcc ggggtgataa ctaacccgag cattatcgct gccagcaagg agtccatctg
    18421 ggaagcgctg ccgcgtctgc aaaaagcgat tggtgatgag ggcattctgt ttgctcagac
    18481 catgagccgc gacgcgcagg ggatggtgga agaagcgaag cgcctgcgcg acgctattcc
    18541 gggtattgtg gtgaaaatcc cggtgacttc cgaaggtctg gcagcaatta aaatactgaa
    18601 aaaagagggt attactacac ttggcaccgc tgtatatagc gccgcagaag ggttattagc
    18661 cgcactggca ggggcaaaat acgttgctcc gtatgttaac cgcgtagatg cccagggcgg
    18721 ggacggcatt cgtacggttc aggagctgca aacgctgtta gaaatgcacg cgccagaaag
    18781 catggtgctg gcagccagct ttaaaacgcc gcgtcaggcg ctggactgtt tactggcagg
    18841 atgtgaatcc atcaccctgc ccttagatgt agcgcaacaa atgctcaaca cccctgcggt
    18901 agagtcagct atagagaagt tcgaacacga ctggaatgcc gcatttggca ctactcatct
    18961 ctaaaggagc aattatggac cgcattattc aatcaccggg taaatacatc cagggcgctg
    19021 atgtgattaa tcgtctgggc gaatacctga agccgctggc agaacgctgg ttagtggtgg
    19081 gtgacaaatt tgttttaggt tttgctcaat ccactgtcga gaaaagcttt aaagatgctg
    19141 gactggtagt agaaattacg ccgtttggcg gtgaatgttc gcaaaatgag atcgaccgtc
    19201 tgcgtggcat cgcggagact gcgcagtgtg gcgcaattct cggtatcggt ggcggaaaaa
    19261 ccctcgatac tgccaaagca ctggcacatt tcatgggtgt tccggtagcg atcgcaccga
    19321 ctatcgcctc taccgatgca ccgtgcagcg cattgtctgt tatctacacc gatgagggtg
    19381 agtttgaccg ctatctgctg ttgccaaata acccgaatat ggtcattgtc gacaccaaaa
    19441 tcgtcgcggg cgcacctgca cgtctgttag cagcgggtat cggcgatgcg ctggcaacct
    19501 ggtttgaagc gcgtgcttgc tctcgtagcg gcgcgaccac catggcgggc ggcaagtgca
    19561 cccatgcggc gctggcactg gctgaactgt gctacaacac cctgctggaa gaaggcgaaa
    19621 aagcgatgct tgccgccgaa cagcatgtag taaccccggc gctggagcgc gtgattgaag
    19681 cgaacaccta tttgagcggc gttggttttg aaagcggcgg ctggcggcgg ctcacgcggt
    19741 gcataacggc ctgaccgcta tcccggacgc gcatcactat tatcacggtg aaaaagtggc
    19801 atttggtacg ctgacgcagc tggtactgga aaacgcgccg gtggaggaaa tcgaaaccgt
    19861 agctgcactt agccatgcgg taggtttgcc aataactctc gctcaactgg atattaaaga
    19921 agatgtcccg gcgaaaatgc ccatgcgacg ccagatcagg tttacgccgc actgctggta
    19981 gctgaccagt acggtcagcg tttcctgcaa gagtgggaat aacctactcc aaactcccgg
    20041 cttgtccggg agtttgaacg caaaattgcc tgatgcgcta cgcttatcag gcctacgcaa
    20101 tctctgcaat atattgaatt tgcgtgcttt tgtaggccgg ataaggcgtt cactccgcat
    20161 ccggcactct gtgccagtaa tctgaaactc ccacgttgtt caggagtttt ttatccgctt
    20221 ctggacattt tctccacaga acaatcgtta gcccggaagg tcgaatctgt gactcttatt
    20281 ttcacggacg aaccagatga aagcttcact ggcgctgctc agcctgctaa cggcatttac
    20341 cagccactcc ttaaaatctc ccgccgttcc accaaccgtg gtacaaatct aggccaatac
    20401 caaccttgcc atcgcagacg gtgccagaca gcaaattggc agcacgctat tttacgatcc
    20461 ggcgtatata cagcttacct atcctggcgg tgatgttccg caagaacgcg gtgtatgttc
    20521 cgatgtggtg atccgcgcat tgcgcagcca gaaggtcgat ttgcagaaac tggttcacga
    20581 agatatggcg aagaattttg ctgagtaccc gcaaaaatag aagttaaagc gcccggacag
    20641 caacatcgat caccgtcggg tgcctaatct ggaaacctgg tttagccgcc acaacaaaac
    20701 acgccccatc agtaaaaatg ccggtgacta tcaagcgggc gatattgtct cctggcgatt
    20761 agataacggg ctggcgcata ttggggtggt gtcagatggc ttcgcccgcg acggtacgcc
    20821 gctggtgata cataacattg gcgcaggcgc gcaggaagaa gatgtgctgt ttagctggcg
    20881 gatggtgggg cattatcgtt attttgttaa ataagagatc gccccacatg aagaaaaaat
    20941 tcagatgctt tttacactga cagcttcttt tttcttacgg ctcaccagcg ccagtgccag
    21001 cacaatcagc acaataccgc taccttcaac ggcccccgga ttctcaccga gcagccacca
    21061 ggaaaagagt acgccacaaa cgggaacggc cagtgtgctt aagctggcaa tactggcagg
    21121 caagtttttc agcacaaaca gccataagct ccacgctagc gccgtcgcca gaatcgcact
    21181 gtaggccagc gcccagaaca cggtgggctg ccagtcaatt tcacgttgcg gcaccagtaa
    21241 agcgaccaca ctcatcacca gcgccgcata cagcatctgc caggatgtta acgataataa
    21301 atccacgcgc ggatgacggg cgtataggcg tttagcaaca atcgcgctcg ccccccaact
    21361 aacaccggag aggattgcca gcatggcact tttcatcgaa gagaaatcga gttgccacgg
    21421 ctgcaacacc aaaaataagc cgaaagcggc aatcagaatc gcgaaatatt gcccacgtcg
    21481 caggcgttca ccgagaaaca acgcggcgaa aatcaccacc cagaacggca tggtatagct
    21541 caggatcgcc accttccccg cacctccgct gaccaacgcc cactgcgcca gaccaaccat
    21601 cccgcaggtt tgtaacaggg caatggctaa ggtgtattta aacggcgtcg ggcgcattcc
    21661 gcgaccacgt aataaaagga cgatgaataa aacgagagcg ccgaaaatgc agcgtaaggc
    21721 ggtaaagtcg aaggcaccga tgtaactggt gacttgcttc atgaaaatcc agctataact
    21781 ccagataagt gtgagcacaa ccaggccact gattgccagt gggttgctct ttcctgcggc
    21841 agacatgaga gatccagtgt gtagttttcc tgtggacact atacgccggt aaagaaactt
    21901 cagcgactgc ctgaaagaca gtcgctgaag aaggtcagat tacagcaggt cgaaacggtc
    21961 gaggttcatc actttcaccc atgccgctac gaagtcttta acaaacttct cgtgggcatc
    22021 gctgctggcg taaacttccg ctactgcacg caggacagag ttagaaccaa ataccagatc
    22081 cgcacggctg gctgtgtatt tcacttcgcc ggtttcgcgg tcacgtcctt cgaacagctc
    22141 tttcgattcg tcagtcgctt tccactcgta acgcatatcc agcaagttca cgaagaagtc
    22201 attgctcaat acgccaacgc ggtcagtgaa gacgccgttt ttgctgccat cgaagttggc
    22261 acccagtaca cgcatcccgc ccaccagcgc agtcatttcc ggcgcggtca gcgtcagttg
    22321 ctgtgctttg tcgatcagca gtgactcggt ggtggaaacg tccagacgag cgcgatagtt
    22381 acggaaaccg tcagcaattg gctccagcag ctcaaacatc tcaatgtcag tctgatcctg
    22441 acgcgcatca acgcgacccg gcgcaaacgg tacatgaatg ctcaaacctg cggcgcttgc
    22501 ggctttctca acaccaacca caccagccag cacgatgata tccgccagcg aggctttacc
    22561 agactctttc tggattttct ccagaacagg cagagcacga acggctgcgg cgttcacatc
    22621 ccagtcgcgc tgcggcatta atgccagacg cgcaccgttg gcaccaccgc gtttgtcgcc
    22681 accacggaag gtagaagcag atgcccaggc caccgatacc agctcactaa cagacagacc
    22741 agaatccgca atcgcgaatt tcagatcgat aatgtcctgc tcggtcgggt tgtagatcgg
    22801 ctgcggcagc ggatcttgcc agatcagatc ttctttcggc acttccggcc cgatgtagcg
    22861 agatttcggc cccatatccc tgtgcgtcag tttgaaccag gcacgggcaa aggcttcgtt
    22921 gaacgcctgc ggatcgttga ggaaacgacg agagatcttc tcgaactcag gatcaaaacg
    22981 cagcgtcagg tcggtcacca gcattgtcgg tttacgtttc ttcgacggat cgaacggatc
    23041 cgggataatt tccggtgcgt ctaccgcttc gaactggatt gctccagccg gactgcgggt
    23101 ctgtacccac tcatacttga acagattctc gaagaaatag ttgctccact gggtcggcgt
    23161 ctgggtccag actacttcca gaccagaggt aatggcatct gcgccaacgc cgctgccgta
    23221 agtgctcgcc cagcctaaac cttgttcttc aatcggtgca gcttctggat caggacctac
    23281 gtttgatgtc ggaccggcac cgtgggtttt acccagcgta tgaccacccg caatcagcgc
    23341 cacggtttct tcgtcgttca tgcccatgtt gccgaaggtc gcgcggatag ctgctgccgc
    23401 agaaagcggt tcgccgctgt gatccgggcc ttccgggtta acgtaaatca ggcccatctc
    23461 ggttgcaccc agcggtgctt tcgccagcgc ttccggatga cggtgagtca gccaggcttt
    23521 ttcatcaccc cagttaacat ccagatccgg ttcccagacg tcttcacgac cggcaccaaa
    23581 accgaaggta cggaagccgg agttttccag cgccacgtta cctgcgagga taaacaggtc
    23641 ggcccaggag attttctgac catatttctg tttgattggc cacaacaggc gacgcgcttt
    23701 atcgaggctt acgttatccg gccaggagtt cagcggtgca aaacgttgct gaccacgacc
    23761 cgcgccgccg cgtccatcga ttgaacggta agtacctgcg ccgtgccaag ccatacgaat
    23821 aaacagaccg gcgtagctgc cccagtcggc tggccaccac ggctgagatt ctgtcagcag
    23881 ggctttcaga tcttttttca ggccgtagta atctaatttg ctgaattctt tgcggtagtc
    23941 aaagtcctca cccagcgggt tagaacgatt agaatgttgg tttaacaggt caacacggag
    24001 ttgatttggc caccagtcgc gagtggttgt gcccgccccc gcgctctggt cgtgaccgcc
    24061 ctgatggaac gggcatttgc cagtggctgt ggtgttatgg atatcgtctg acgtgctcat
    24121 caatgtgctc ccctctacag tgttaccgtt acgatacaca gcgttagaga gaagttataa
    24181 ttgaattaat ccacggatgc gatagttgag atcttacata ttgttggtta aagagatgta
    24241 gatcaaattg atcgtaatta agcttttttg attatgtccg aattcggaca aatgctttat
    24301 aaaaaagggt tttagcttca tatccttcaa gtaatgaggg agaaaacaag gctgacaacc
    24361 ttattttccc gccctcattt cgaggcagca ttttgtgctc tgtttaaaat ttgtgatcac
    24421 tgtgtgattt tcacaaaagc cacactattt ataaaccagg tcgaacaccc agcgtatggc
    24481 aaatcgcgta actcatttca gagcggttaa gcgtatagaa gtggaaatct ttcactcctt
    24541 cacggcttaa aatcttcacc atatccatgg caatattcgc gcccaccagt ttgcgggttt
    24601 cggcatcgtc atccagcccg tcaaacattt gcgccatcca cgccggaata cgcacgttgg
    24661 tcatgtcggc aaatttcttc gcctgtttaa agttagatac cggcaaaatg cccgggatga
    24721 tttccacatc aatgcccgcc gatacacagc ggtcacgaaa acgcaggtag ctttcgacat
    24781 cgaagaagaa ctgagtaatc gcgcggttgg ctccggcatc cactttgcgt ttcagattaa
    24841 gcaaatccgc ctgagcgctt tttgcttccg ggtgaacttc cggatacgcc gccacggata
    24901 tatcgaaatc tgccacttct tttaacagcg tcaccaggtc agaagcatac atttctggct
    24961 taccacttcc cggcggcaga tcgccacgca gtgccacgat atgacgaata ccgttattcc
    25021 agtagtcgcg tgcaatggtg cgcagctcgt cgggcgtcgc atcaatgcaa gtaagatgcg
    25081 gtgccgcttc cagaccagtg cgatctttaa tgcctttaat aatgctgtgc gtacggtcgc
    25141 gctcgccgga gttcgcgcca taggtcaccg atacaaactt cggtttcagg ctgctaaggc
    25201 gatcgatgga gttccacagg gtctgctcca tttcactggt acgcggcggg aaaaactcga
    25261 acgaaacgtt aatctgcccc tggacttctg ccaggctctg attcagggca tcccgctggc
    25321 tggcgtgaaa aaagctcata ccttacctca tcaatcgctt gtagttatat gttgtgttgt
    25381 gcacatctat ccatttagac gtccagatgt aaagatgaag gaaaagccga agggcgtcaa
    25441 ccgaaaaatg accaattatg gtgaggaaaa atcagggaag atgaaaaaac ttcaggatgg
    25501 taagaaaaag aaaatccgat atgcaaagca aaccggactg caaatataaa ctaaccatgt
    25561 acttattgct tcttgccgga ttcgtcgtaa acgcttcatc cggcctaatt ctatgataat
    25621 tcaatatatt tcattagaaa ccatagaccc gaaaacatga gtaccgggca ttattaaatt
    25681 tctgaaatta caacaactgt gccagccggt tgatatccga ctgaatcgcc ccggcggtga
    25741 cgtcgcgccc agcaccaggc ccacggatca ccagcggatt atcgcggtac cagcggcttt
    25801 cgatggcaaa gacgttatcg catggcagca gtgatgccag cggatggtct tcacgcaccg
    25861 cctccacgcc cacacgcgct ttgccgttgg catcgaaacg cgccacgtag cgcagcacca
    25921 gccccatttc gcgggccgct tccagccgtt gcaccatctg ctcgttaagt tcatcgccat
    25981 tttcaaagaa atggtcgatg ctgccgcctt cgcaatgagc aggcaccagc gattccacac
    26041 gtacctggtc cggttcaatg ttgtaacccg cttcacgcgc cagaatcacc agcttgcgca
    26101 ttacgtcttt gccggagagg tcgtcacgcg gatcaggctc ggttaagccc tgctgccacg
    26161 cctgatccac cagctcggta aacggcacgc taccgtcgaa ttgcaggaac agccaggaga
    26221 gcgtgccgga gaagatccca ctgatcgaca aaatggtatc gccgctgtcg atcagatcgc
    26281 gcacggtatg gttgatcggc aagcccgcac caacggtggc gttgtatagc cagtgacgcc
    26341 cggttttttc gaaggcgtcg tggatctggc gatatttatt gctgtcgctt gctcccgcca
    26401 gtttgttggc actgatgacg tggaaaccgt ggctggcgaa atcgagatac tgatcagcaa
    26461 gctgctggct ggcggtaacg tccaacacca ctaaatcatc atacggatgg gcgcgcatcc
    26521 acaggaaaag cgactcttca tcctgctcga ccgcttcatc gttgaagaag gctaacgcgc
    26581 ggctggcgtc cagcccgtca taactcaaca gactgcggcg gctgtccacc acgcctgcca
    26641 gcacaaattc aaagccggta cgcgccgaaa gcgtgctctg ctcgcgggcg aacagttcca
    26701 gccagcgaga gccgatattg cctttgccga acaataccag gccgatgcgt ttttctgcgc
    26761 ggaagacgga ctgatgcagc ccctgaatca ggctttcggt cgggccggtg cgcagtactg
    26821 ccaccagact gatgccgtca tcggactgcc acgtaaactc gaccggctgg cctttcagtt
    26881 gctgccagaa gcggtggcaa tgcagcgggt tacgggtgac acccgccccg accatcgcca
    26941 ccagcgccag cccctgacgc aggcgcagtt ctccaggtaa tcccgcttcg tcgaggattt
    27001 tcagcgcact gtcggccact tctgaggtgt agcaaaattg cagcaactgg cgatcgttat
    27061 gcacgccaac cgccagtgga cgcacctggg cacgtttcag gatctggtcg atctctttat
    27121 gcgccagttt gaaatcctga ctggcgggca cctgaaactc aatcaaacag acatcatcgt
    27181 ggctggtgac aatacgcgca ccagtaccgg aggccagcac gcgttcaatg cgcgtggaac
    27241 cttgatccgg cgtgtagcta cagcgcagct gcaggtcgat ttcgctgcca gaaactggct
    27301 gtaaagtacg ggcgtgaaga acgggagccg ccagacgcgc cagttcgctg gcctcatcca
    27361 gacgcagcaa cggcagcagg caggcatctt tcactttacg cgggtcggca ctgtataccc
    27421 cggcgacgtc gctccagatg gttacacgag aaacacccgc cagcgcaccg atttgtgtcg
    27481 cggaatagtc ggaaccgtta cgccccagca acaccgtttc accggcgttg ttacggctga
    27541 taaacccggt caccaccaga cgtttgctcg gatgttgtac cagtagctgt tgcagcaacg
    27601 ggtaagaaag cccttcatca acctgcggtt gcgcagcgcg ttcagcgcgt aaaaactcac
    27661 gggcatcaag ccaggccgct ggcagccctt gttgattaag taccgcagac atcaggcgcg
    27721 ccgaccacac ttcaccgtgg cccaccactt ccgcatacac cgcatcgtta ataccactgt
    27781 cgagcaacgc cgccaggcgc tcaagatcgc tgacaaaagc gctaatgagg ctatcggctt
    27841 cttcagcggg tagcagaccg ctaatcagat cgcactgata gcgacgcagc gtttgttgaa
    27901 cctgatgcgc agagagacga tcggtctggc ttagtttcaa ccagttaatc aactggttag
    27961 tggtgctacc ggcggcggaa accaccatca tatcgtcagg ctgagagaac tccgccataa
    28021 tgcccgcgac acgcaaataa cacttcacat cagccagact actgccacca aatttatgca
    28081 gctgacgacc tttcgcccct gcctgcgcaa tcacactcat ttttacccct tgtttgcagc
    28141 ccggaagcca ttttccaggt cggcaattaa atcttcgcca tcttcaatac cggtggagat
    28201 acgcagcagc gtctcggaga tcccggcggc agcacgcgct tctggtgcca tgcctgcatg
    28261 tgtcatggtt gcggcgtgag agattaaact ttccactccc cctaatgatt ccgccagcgt
    28321 aaacaacgac agcccgccca ggaaacgaca cagcgtctgc tcatcgccat ccagttcaaa
    28381 actcaacatt gcgccaaaac ctttttgctg acgcgcggca atttcatgcc cctgattttc
    28441 cggcagcgac gggtgataca gttttttcac caacggctgg gtttgcagat atttcacaat
    28501 cgcctgcgcg ttgcgctgcg ccagctccat acgcggcacc agcgttcgca acccgcgtag
    28561 cagcagatag ctgtcaaacg cgccgcccgt cacgccaata ttgtttgccc accaggcaag
    28621 ttcagtgaca acgtccgggt ctttagcaat caccacgcca gccaccacgt cagagtgacc
    28681 gttcagatat ttcgtacatg aatgcaacac cagatcggca cccaatgcca gcggattttg
    28741 taatgccggg cttaagaagg tgttatccac cacgctcacc gccccgactt cccttgccag
    28801 atggcagatt ttcgcaatat ccacgacgcg taacaatgga ttacttgggc tttctaccag
    28861 taccagtttg ggtttttctg ccagcgctgc ccgtaatgca tgttcatcgc cttgatcaac
    28921 aaacaacacg cgatagcaac cgcgtttcgc cagactgtcg aacaggcgat agctaccgcc
    28981 gtagcagtcg tgcggcgcaa ccagcagatc gccaggtttc aaaaagacgg tcgttaccag
    29041 gtgaatcgcg gacatgccgg tattagtaag tactgcgcca gcaccgcctt ccagttctgc
    29101 cagcgcacgc tgaaccacat cgcgcgttgg gttgccgcga cgcgagtaat catgcgcgcg
    29161 cggttcatta aatccggtaa agttataggt gctggaaaga tggatgggag ggacaacgca
    29221 accatactgt tcgtcgtcat ttaacccgct acgcactgcg atggtggcct gtttacgcgt
    29281 catgtgatga agtttcctgg gctttgtcgg tgaaatgtca ggcaccagag taaacattgt
    29341 gttaatggac gtcaatacat ctggacatct aaacttcttt gcgtatagat tgagcaaatc
    29401 ccaaatagcc gttaaaatta tatgcattat cacgccgaca ggtgcattac acgatgtcac
    29461 ggtaacgcct gtacggtaaa ctatgcgggt ttacggtcag tacccacata aattgtgggg
    29521 tctggtctca atttattgac gaagaggatt aagtatctca tggctgaatg gagcggcgaa
    29581 tatatcagcc catacgctga gcacggcaag aagagtgaac aagtcaaaaa gattacggtt
    29641 tccattcctc ttaaggtgtt aaaaatcctc accgatgaac gcacgcgtcg tcaggtgaac
    29701 aacctgcgtc acgctaccaa cagcgagctg ctgtgcgaag cgtttctgca tgcctttacc
    29761 gggcaacctt tgccagatga tgccgatctg cgtaaagagc gcagcgacga aatcccggaa
    29821 gcggcaaaag agatcatgcg tgagatgggg attaacccgg agacgtggga atactaaagc
    29881 ggaaaagagc ggcgcggagt ggaatcgcct gatgcactac gcttatcagg cctacgtcat
    29941 attgcaattt attgaatttg cacgaacttg taggccggat aaggcgttca cgccgcatcc
    30001 ggcataaaca acgagcacgt tgtctgcgac ccaccgcttt ttatacatgg acgtttaact
    30061 atgaaaaaca ggctgctgat cctcagcctg ctggtttctg tacctgcctt tgcctggcag
    30121 ccgcaaactg gcgacatcat ctttcaaatc tctcgctcat cgcaaagtaa agcgatccaa
    30181 ctggcaaccc attccgatta cagccacacc ggtatgctgg tgatgcgtaa caaaaagccc
    30241 tacgtttttg aagcggtcgg cccggtgaaa tacaccccgc tcaagcagtg gattgcccat
    30301 ggtgaaaagg gcaactacgt tgttcgccgc gttgaaggcg gactgagtgt tgaacaacag
    30361 caaaaactga cgcaaacggc aaaacgttat ctcggtaaac cgtatgattt cagcttctca
    30421 tggagcgacg atcgccagta ctgttcggaa gtggtgtgga aggtttacca gaacgcgctg
    30481 ggaatgcgcg tgggcgaaca gcagaagttg aaagagtttg atctcagcaa cccgctggtc
    30541 caggcgaagc tcaaagaacg ctacggtaaa aatattccgc ttgaggaaac ggtagtttca
    30601 ccacaagccg ttttcgacgc gccacaactc accacggttg ccaaagagtg gccgctgttt
    30661 tcgtggtgat acgaccaaaa ttcaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga
    30721 aatccggtct tatttgctgc ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc
    30781 accggtagca acatcacgct gtttgccagt gaagaacggg tggcacttgc tgcacacgtc
    30841 gaggttcagg tcatgaccaa cggtggagcg gattttcatt acgttaccgc aagagcagct
    30901 agcagtaatt tcttcgtatt tcgggtgaat atcttttttc atgggaaaac ctcggtttaa
    30961 ggccgcgtcg ctcttccagc cctaacgcca gacaccacgc gatgttaaaa gtatagcttc
    31021 aatacgatca tttcgtacga aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac
    31081 tgatccgcac tcttctacgg caatgtgtat actaacccac cgaatttcaa gtcaggatga
    31141 tgctatgccc gttgcccacg ttgccttgcc cgttccgctt cctcgtacct ttgactatct
    31201 gctgccagaa ggcatgacgg ttaaagctgg gtgtcgcgtg cgcgtgccgt ttggcaaaca
    31261 gcaggagcgc atcgggattg tggtatcagt tagcgatgcc agcgaactgc cgctcactga
    31321 gctaaaagcg gtagtcgaag tgctggatag tgagccggtg tttactcact ccgtctggcg
    31381 attgctgcta tgggcggcag attactatca tcatccgatt ggcgatgtgc tgtttcatgc
    31441 cttgccgatt ttactacgcc aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt
    31501 tgccactgaa caaggtcagg cggtggatct gaacagcctg aaacgctcac ccaagcaaca
    31561 acaggcgctg gcagcgttac ggcaaggcaa aatctggcgc gaccaggttg ccacgctcga
    31621 atttaatgat gccgcgttgc aggcgctgcg caaaaaaggt ctgtgtgatt tagcaagtga
    31681 aacaccagag tttagcgact ggcgaacgaa ctatgccgtt tctggtgagc ggttgcgatt
    31741 gaataccgaa caggccaccg ccgttggcgc aattcatagc gcggcagata ctttttctgc
    31801 ctggctgctg gcgggcgtta ccggttccgg taaaacggag gtttatctca gtgtactgga
    31861 aaacgtgctc gctcagggca aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc
    31921 gcaaactatc gcccgttttc gtgaacggtt taatgcgccc gtggaagttc tgcattccgg
    31981 tctgaacgac agcgagcgtc tttcggcgtg gctgaaagcg aaaaatggcg aggcggcgat
    32041 tgtgatcggc acccgctccg cgctgtttac gccgtttaaa aatctcggcg tgattgtcat
    32101 tgacgaagag cacgacagtt cctacaagca gcaggaaggc tggcgctatc atgcccgcga
    32161 cctggcggtg tatcgtgcgc atagcgagca aatcccgatt attcttggct ccgcaacgcc
    32221 cgcgctggaa acgttgtgca acgtccagca gaaaaaatac cgcctgctgc acctgacgcg
    32281 ccgggcgggg aatgcgcgtc cggcaattca acacgtgctg gatttaaaag gtcagaaggt
    32341 gcaggcaggt ctggctccgg cgttaatcac ccgtatgcgc cagcatttac aggccaacaa
    32401 tcaggttatc ctctttctta accgccgtgg ctttgcgcct gcgctactgt gccacgactg
    32461 cggctggatt gccgaatgcc cacgttgcga tcactactac acgctgcatc aggcgcaaca
    32521 gcatttgcgc tgtcaccact gtgacagtca gcgtccggtg ccgcgccagt gcccttcctg
    32581 cggttccacg cacctggtac ccgtggggct gggcaccgaa cagcttgaac agacgcttgc
    32641 gccgctgttc cctgatgtgc ccatttctcg tatcgaccgc gataccacca gccgcaaagg
    32701 ggcgctggaa cagcaactgg cggaagtaca tcgcggcggt gcgaggattt tgatcggcac
    32761 acaaatgctg gcgaaaggtc accactttcc ggatgtgacg ctggtggcgt tactggatgt
    32821 ggacggcgcg ctgttttctg ccgattttcg ctcggcagag cgtttcgctc agctttacac
    32881 ccaggtcgct ggtcgtgccg ggcgtgcggg taaacaaggc gaagtggtgc tgcaaacgca
    32941 ccatccggaa catcctctgt tgcaaacgtt gctctataaa ggctacgacg cctttgccga
    33001 acaggcgctg gctgagcgac ggatgatgca actgccgcca tggaccagcc atgtgattgt
    33061 gcgtgcggaa gatcataaca atcagcatgc gccgctgttc ctgcaacaac tgcgtaatct
    33121 gatcctctcc agcccgctgg cagacgacaa actgtgggtt ctcggtccgg ttccggcact
    33181 ggcacctaaa cgtggcggtc gctggcgctg gcagatattg ttgcagcacc cttcccgcgt
    33241 gcgcttgcaa cacatcatta gtggtacgct ggcgctcatc aatacaatac cggattcccg
    33301 caaggtgaaa tgggtactgg atgttgatcc gattgagggt taaaccgctt acgatgcgag
    33361 gcggatcgaa aaattcaata ttcatcacac ttttcatgaa aattctgtaa ccgttttcac
    33421 gcgctatctg ctaaaaatgt tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt
    33481 gccaggcgag gagtgagtgt gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac
    33541 gttgccctca aggcaaaagt ctctacagcg actgtctccc gagcattaat gaatcccgat
    33601 aaagtctccc aggccacccg taatcgggtt gaaaaagcgg cccgggaagt gggttattta
    33661 ccgcagccta tggggcgcaa cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc
    33721 ccggatatct gcgatccctt ctttagcgaa attattcgcg gtatcgaagt tacggcggca
    33781 aatcacggat atctggtgct gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc
    33841 tttatcgatt tgatcatcac caagcaaatt gatggcatgt tgctgctggg ttcaaggctg
    33901 ccgtttgatg ccagcattga ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa
    33961 tttgcaccgg aactggagct gcctaccgtt catatcgaca atctgaccgc cgcatttgat
    34021 gcagtaaatt atttatatga gcaagggcat aaacggattg gctgtatagc cggtcccgaa
    34081 gagatgccac tgtgtcacta ccgcctgcaa ggctatgttc aggcgctgtg tcgctgcgga
    34141 attatggttg atccacaata catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa
    34201 gcaatgcaac agctgcttga tcttccacaa ccgcctactg cagtcttctg ccatagcgat
    34261 gtgatggcgc tcggcgcact ttctcaggca aaacgccagg ggctgaaagt cccggaagac
    34321 ctttccataa tcggttttga taacatcgac ctgacgcaat tttgtgatcc gccgctgaca
    34381 accatcgcgc agccgcgtta cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa
    34441 atgcaggggc aacacgttgg cagtggctct cgtttaatgg actgcgaact tatcatccgg
    34501 ggatcaacac gcgcgttacc ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc
    34561 ccgccgctta agtaacatgg cgggctgacg aacgaataaa tacagcgaaa cgatagtggc
    34621 acaacgagat tatgtacgcc gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc
    34681 acggaaaaag caacgaaatc tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc
    34741 cgttcttgtg acctttatcg gaggtctgta cttcattacg catcacaaga aagaagagtc
    34801 cgagacgctg caaagccaga aagtgaccgg aaacggacta ccaccaaaac cagaagaacg
    34861 ctggcgctac attaaagagc tggaaagtcg ccagccgggt gtgcgtgcgc ccacagaacc
    34921 ttctgccggt ggtgaagtga aaacgccgga gcaactgaca ccagaacaac gtcagcttct
    34981 tgaacaaatg caagctgata tgcgccagca gccaacgcag ctggttgagg ttccgtggaa
    35041 tgaacagacg ccggaacagc gtcagcaaac gctacaacgc cagcgtcagg cgcagcaatt
    35101 agcggagcag caacgcctgg ctcagcagtc gcgtacgact gaacaaagct ggcagcagca
    35161 gacgcgtacg tcgcaagccg cgccggtaca ggcgcaaccg cgccagtcta aaccagcttc
    35221 tactcagcag ccgtaccagg atctgctgca aacccctgcg cacacgaccg cgcagtcgaa
    35281 gccgcagcaa gctgcgccgg ttgctcgtgc cgctgacacg ccaaaaccga cggcggagaa
    35341 aaaagacgaa cgccgctgga tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga
    35401 gacggtacgt gctcagctgg cgttcgaagg atttgactcg aaaatcacca ccaacaatgg
    35461 ctggaatcgt gtggtcattg gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa
    35521 tcggttgaag atggcgggtc atacaaactg cattcggctc gccgccgggg gttgaaaccc
    35581 tcaaaatccc ccccatctat aattgcatta tgccccgtac ttttgtacgg ggtttgtact
    35641 ctgtattcgt aaccaagggg tcagctcgtg acaactatag taagcgtacg ccgtaacggc
    35701 catgtggtca tcgctggtga tggtcaggcc acgttgggca ataccgtaat gaaaggcaac
    35761 gtgaaaaagg tccgccgtct gtacaacgac aaagtcatcg cgggctttgc gggcggtact
    35821 gcggatgctt ttacgctgtt cgaactgttt gaacgtaaac tggaaatgca tcagggccat
    35881 ctggttaaag ccgccgttga gctggcaaaa gactggcgta ccgatcgcat gctgcgcaaa
    35941 cttgaagcac tgctggcagt cgcggatgaa actgcatcgc ttatcatcac cggtaacggt
    36001 gacgtggtgc agccagaaaa cgatcttatt gctatcggct ccggcggccc ttacgcccag
    36061 gctgcggcgc gcgcgctgtt agaaaacact gaacttagcg cccgtgaaat tgctgaaaag
    36121 gcgttggata ttgcaggcga catttgcatc tataccaacc atttccacac catcgaagaa
    36181 ttaagctaca aagcataagg atctcccatg tctgaaatga ccccacgcga aatcgtcagc
    36241 gaactggata agcacatcat cggccaggac aacgccaagc gttctgtggc gattgctctg
    36301 cgtaaccgct ggcgtcgcat gcagctcaac gaagagctgc gccatgaagt gaccccgaaa
    36361 aatatcctga tgatcggccc gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct
    36421 aagctggcga atgcgccgtt catcaaagtt gaagcgacca aatttaccga agtgggctac
    36481 gtcggtaagg aagttgattc tattattcgc gatctgaccg atgccgccgt gaaaatggta
    36541 cgcgtccagg ctatcgagaa aaaccgttat cgcgctgaag agctggcaga agaacgtatt
    36601 ctcgacgtgc tgctcccacc tgctaaaaac aactggggac agactgaaca gcagcaggaa
    36661 ccttccgctg ctcgtcaggc attccgcaaa aaactgcgtg aaggccagct tgatgacaaa
    36721 gaaatcgaga tcgatcttgc cgcagcaccg atgggcgttg aaattatggc tcctccgggc
    36781 atggaagaga tgaccagcca gctgcagtcc atgttccaga acctgggcgg ccagaagcaa
    36841 aaagcgcgta agatgaaaat caaagacgcc atgaagctgc tgattgaaga agaagcggcg
    36901 aaactggtga acccggaaga gctgaagcaa gacgctatcg acgctgttga gcagcacggg
    36961 atcgtgttta tcgacgaaat cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat
    37021 gtttctcgtg aaggcgttca gcgtgacctg ctgccgctgg tagaaggttg caccgtttcc
    37081 accaaacacg ggatggtcaa aactgaccac attctgttta tcgcttctgg cgcgttccag
    37141 attgcgaaac cgtctgacct gatcccggaa ctgcaaggtc ggctgccaat ccgcgttgaa
    37201 ctgcaggcac tgaccaccag cgacttcgag cgtattctga ccgagccgaa tgcctctatc
    37261 accgtgcagt acaaagcact gatggcgact gaaggcgtaa atatcgagtt taccgactcc
    37321 ggtattaaac gcatcgcgga agcggcatgg caggtgaacg aatctaccga aaacatcggt
    37381 gctcgtcgtt tacacactgt tctggagcgt ttaatggaag agatttccta cgacgccagc
    37441 gatttaagcg gtcaaaatat cactattgac gcagattatg tgagcaaaca tctggatgcg
    37501 ttggtggcag atgaagatct gagccgtttt atcctataat cgcgttcaat cattttcatc
    37561 attgtttgat ggggctgaaa ggccccattt ttattggcgc gtattatgac tgaacaacaa
    37621 attagccgaa ctcaggcgtg gctggaaagt ttacgaccta aaaccctccc cctcgccttt
    37681 gctgcaatta tcgtcgggac ggcgctggca tggtggcaag gtcacttcga tccgctggtc
    37741 gccctgctgg cactaattac cgccgggcta ttacagatcc tttctaacct cgccaatgat
    37801 tacggcgatg cggtaaaagg cagcgataaa cctgaccgca ttgggccgct acgcggcatg
    37861 caaaaagggg tcattaccca gcaaaagatg aaacgggcgc tcattattac cgtcgtgctc
    37921 atctgtctct ccgggctggc actggttgca gtggcatgcc atacgctggc cgattttgtc
    37981 ggtttcctga ttcttggcgg gttgtcgatc attgccgcta tcacctacac cgtgggcaat
    38041 cgtccttatg gttatatcgg tctgggtgat atttccgtac tggttttctt tggctggttg
    38101 agtgtcatgg ggagctggta tttacaggct catacattga ttccggcact gatccttccg
    38161 gcgaccgcat gcggcctgct ggcaacggca gtactgaata ttaataacct gcgtgatatc
    38221 aatagcgacc gcgaaaatgg caaaaacacg ctggtggtgc gcttaggtga agtgaacgcg
    38281 cgtcgttatc atgcctgcct gctgatgggc tcgctggtgt gtctggcgct gtttaatctc
    38341 ttttcgctgc atagcctgtg gggctggctg ttcctgctgg cggcaccatt actggtgaag
    38401 caagcccgtt atgtgatgcg ggaaatggac ccggtggcga tgcgaccaat gctggaacgt
    38461 actgtcaagg gagcgttact gactaacctg ctgtttgttt tagggatatt cctaagccag
    38521 tgggcagcat aactgacaaa tatcaattaa caattgatga ttttgccaac agcccacata
    38581 gcgcgatata ctgaaaattc tcgcagcaac tgaatgttaa tcctatgaaa tacgatactt
    38641 ccgagctttg tgacatctat caagaagatg ttaacgtcgt ggaaccgctg ttctccaact
    38701 ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt aaaatgtttc gaggacaacg
    38761 ggttgctgta cgatctgctc gaacagaatg gccgtggtcg tattcttgtc gtcgatggcg
    38821 gtggttctgt tcgtcgcgca ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg
    38881 aatgggaagg tctggtcatt tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg
    38941 atatcggcat ccaggcgatg gcggcaattc cggttggtgc cgctggcgaa ggcattggcg
    39001 aaagcgatgt ccgcgtcaat tttggcggtg tcaccttctt ctccggcgac catctttatg
    39061 ccgacaatac cgggattatt ctttcagaag atccgctgga tattgaatga taagaaaggc
    39121 accgcaaggt gccttttttc tgcgttacct gttggcctac acagtaaaga aattacgcga
    39181 aagatgaagc gtaatcagac ctcttccatg cgacccagca gggcctgcag acgttcctgc
    39241 cagccgttct gctgttcttt cagatggttg ttctcacgct ccagctcttc gcgctgatgc
    39301 tgggcatttt gaacttcctg cgacagtgag ttgttttttt ctttcagctc ttcgatttcc
    39361 atctgcaaca gagtgatggt atcaatcgcc tgctgtactt ttgcttccag tttctcaaac
    39421 acttctaatg acattgtcat acctctcctg aattgcaagg cgttgatgga taaaaaatcc
    39481 tcgtcccgat taccggtgac gccttaataa atacgagcgc actttagtta gctccgattg
    39541 tatgaagccg cgccatcgct gtccagcggc acgccttgca gattacggtt tgccacactt
    39601 ttcatccttc tcctagtgac ataatccaca tcaatcgaaa atgttaataa atttgttgcg
    39661 cgaatgatct aacaaacatg catcatgtac aatcagatgg aataaatggc gcgataacgc
    39721 tcattttatg acgaagcaca cacattttaa gttcgatatt tctcgttttt gctcgttaac
    39781 gataagttaa cagcatgcct acaagcatcg tggaggtccg tgactttcac gcatacaaca
    39841 aacattaact cttcaggatc cgattatgag tcaaacatca accttgaaag gccagtgcat
    39901 tgctgaattc ctcggtaccg ggttgttgat ttttttcggt gtgggttgcg ttgcagcact
    39961 aaaagtcgct ggtgcgtctt ttggtcagtg ggaaatcagt gtcatttggg gactgggggt
    40021 ggcaatggcc atctacctga ccgcaggggt ttccggcgcg catcttaatc ccgctgttac
    40081 cattgcattg tggctgtttg cctgtttcga caagcgcaaa gttattcctt ttatcgtttc
    40141 acaagttgcc ggcgctttct gcgctgcggc tttagtttac gggctttact acaatttatt
    40201 tttcgacttc gagcagactc atcacattgt tcgcggcagc gttgaaagtg ttgatctggc
    40261 tggcactttc tctacttacc ctaatcctca tatcaatttt gtgcaggctt tcgcagttga
    40321 gatggtgatt accgctattc tgatggggct gatcctggcg ttaacggacg atggcaacgg
    40381 tgtaccacgc ggccctttgg ctcccttgct gattggtcta ctgattgcgg tcattggcgc
    40441 atctatgggc ccattgacag gttttgccat gaacccagcg cgtgacttcg gtccgaaagt
    40501 ctttgcctgg ctggcgggct ggggcaatgt cgcctttacc ggcggcagag acattcctta
    40561 cttcctggtg ccgcttttcg gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg
    40621 caaactgatt ggtcgccatt tgcctttcga tatctgtgtt gtggaagaaa aggaaaccac
    40681 aactccttca gaacaaaaag cttcgctgta atatgactac gggacaatta aacatgactg
    40741 aaaaaaaata tatcgttgcg ctcgaccagg gcaccaccag ctcccgcgcg gtcgtaatgg
    40801 atcacgatgc caatatcatt agcgtgtcgc agcgcgaatt tgagcaaatc tacccaaaac
    40861 caggttgggt agaacacgac ccaatggaaa tctgggccac ccaaagctcc acgctggtag
    40921 aagtgctggc gaaagccgat atcagttccg atcaaattgc agctatcggt attacgaacc
    40981 agcgtgaaac cactattgtc tgggaaaaag aaaccggcaa gcctatctat aacgccattg
    41041 tctggcagtg ccgtcgtacc gcagaaatct gcgagcattt aaaacgtgac ggtttagaag
    41101 attatatccg cagcaatacc ggtctggtga ttgacccgta cttttctggc accaaagtga
    41161 agtggatcct cgaccatgtg gaaggctctc gcgagcgtgc acgtcgtggt gaattgctgt
    41221 ttggtacggt tgatacgtgg cttatctgga aaatgactca gggccgtgtc catgtgaccg
    41281 attacaccaa cgcctctcgt accatgttgt tcaacatcca taccctggac tgggacgaca
    41341 aaatgctgga agtgctggat attccgcgcg agatgctgcc agaagtgcgt cgttcttccg
    41401 aagtatacgg tcagactaac attggcggca aaggcggcac gcgtattcca atctccggga
    41461 tcgccggtga ccagcaggcc gcgctgtttg gtcagttgtg cgtgaaagaa gggatggcga
    41521 agaacaccta tggcactggc tgctttatgc tgatgaacac tggcgagaaa acggtgaaat
    41581 cagaaaacgg cctgctgacc accatcgcct gcggcccgac tggcgaagtg aactatgcgt
    41641 tggaaggtgc ggtgtttatg gcaggcgcat ccattcagtg gctgcgcgat gagatgaagt
    41701 tgattaacga cgcctacgat tccgaatatt tcgccaccaa agtgcaaaac accaatggtg
    41761 tgtatgtggt tccggcattt accgggctgg gtgcgccgta ctgggacccg tatgcgcgcg
    41821 gggcgatttt cggtctgact cgtggggtga acgctaacca cattatacgc gcgacgctgg
    41881 agtctattgc ttatcagacg cgtgacgtgc tggaagcgat gcaggccgac tctggtatcc
    41941 gtctgcacgc cctgcgcgtg gatggtggcg cagtagcaaa caatttcctg atgcagttcc
    42001 agtccgatat tctcggcacc cgcgttgagc gcccggaagt gcgcgaagtc accgcattgg
    42061 gtgcggccta tctcgcaggc ctggcggttg gcttctggca gaacctcgac gagctgcaag
    42121 agaaagcggt gattgagcgc gagttccgtc caggcatcga aaccactgag cgtaattacc
    42181 gttacgcagg ctggaaaaaa gcggtcaaac gcgcgatggc gtgggaagaa cacgacgagt
    42241 aatgtcaggc acctgaatcg attgcctgat gctgatcgcc tgatgcgacg cttacgcgtc
    42301 ttatcaggcc tacgagccaa tgccattttg tgtaggcggg ataaggcgtt cacgccgcat
    42361 ccggcatcac gcagactctg aaaccgtgcg ggggcaaccc cgcacacatc aataatccca
    42421 cccttctcct gtgctacact tcgcgccatt ccttactgct tagagtttgc tatgagacga
    42481 gaacttgcca tcgaattttc ccgcgtcacc gaatctgcgg cgctggctgg ctacaaatgg
    42541 ttaggacgcg gcgataaaaa caccgcggac ggcgcggcgg taaacgccat gcgtattatg
    42601 ctcaaccagg tcaacattga cggcaccatc gtcattggtg aaggtgaaat cgacgaagca
    42661 ccgatgctct acattggtga aaaagtcggt actggtcgcg gcgacgcggt agatattgct
    42721 gttgatccga ttgaaggcac gcgcatgacg gcgatgggcc aggctaacgc gctggcggtg
    42781 ctggcagtag gcgataaagg ctgcttcctc aatgcgccgg atatgtatat ggagaagctg
    42841 attgtcgggc cgggagccaa aggcaccatt gatctgaacc tgccgctggc ggataacctg
    42901 cgcaatgtag cggcggcgct cggcaaaccg ttgagcgaac tgacggtaac gattctggct
    42961 aaaccacgcc acgatgccgt tatcgctgaa atgcagcaac tcggcgtacg cgtatttgct
    43021 attccggacg gcgacgttgc ggcctcaatt ctcacctgta tgccagacag cgaagttgac
    43081 gtgctgtacg gtattggtgg cgcgccggaa ggcgtggttt ctgcggcggt gatccgcgca
    43141 ttagatggcg acatgaacgg tcgtctgctg gcgcgtcatg acgtcaaagg cgacaacgaa
    43201 gagaatcgtc gcattggcga gcaggagctg gcacgctgca aagcgatggg catcgaagcc
    43261 ggtaaagtat tgcgtctggg cgatatggcg cgcagcgata acgtcatctt ctctgccacc
    43321 ggtattacca aaggcgatct gctggaaggt attagccgca aaggcaatat cgcgactacc
    43381 gaaacgctgc tgatccgcgg caagtcacgc accattcgcc gcattcagtc catccactat
    43441 ctggatcgca aagacccgga aatgcaggtg cacattctct gagtgatttg atcgattgag
    43501 ccttccagtc cttcgggact ggaatttttt tgttcggaga acgaagataa ggcaagtcaa
    43561 tcaaaacagg agaaaaacat ggctgattgg gtaacaggca aagtcactaa agtgcagaac
    43621 tggaccgacg ccctgtttag tctcaccgtt cacgcccccg tgcttccgtt taccgccggg
    43681 caatttacca agcttggcct tgaaatcgac ggcgaacgcg tccagcgcgc ctactcctat
    43741 gttaactcgc ccgataatcc cgatctggag ttttacctgg tcactgtccc cgatggcaaa
    43801 ttaagcccac gattggcggc actgaaaccg ggcgatgaag tgcaggtggt tagcgaagca
    43861 gctggcttct ttgttctgga tgaagtaccg gactgcgaaa cgctatggat gctggcaacc
    43921 ggtacagcga ttggccctta tttatcgatt ctgcaactag gcaaagattt agatcgcttc
    43981 aaaaatctgg tcctggttca cgccgcacgt tatgccgccg acttaagcta tttgccactg
    44041 atgcaagaac tggaaaaacg ctacgaaggc aaactgcgca ttcagacggt ggtcagtcgg
    44101 gaaacggcag cggggtcgct cacgggacgg ataccggcat taattgaaag tggggaactg
    44161 gaaagcgcga ttggcctgcc gatgaataaa gaaaccagcc atgtgatgct gtgcggcaat
    44221 ccacagatgg tgcgcgatac acaacagttg ctgaaagaga cccggcagat gacgaaacat
    44281 ttacgtcgcc gaccgggcca tatgacagcg gagcattact ggtaagcggt tacttatcga
    44341 taaacggcac gatgagcaaa tccgcactca tcttattgat catcccgcga tatgccggca
    44401 tcaaacggtt gataaatgag tgatgatgac cacagacaag gaggtcgcac tgctcttttt
    44461 gcataatttc cagcagtgtt tccggcattt ctccgcgttc aatacgcagt tttgtcttcg
    44521 gccattgaat atttttcgtc agtttataca gcttgttatc cgacttattc ttcaacaatt
    44581 gaagaatatc ttctgttgca gggaagtaga tacccggata caactcgctt aagccatcat
    44641 caatatgaat taacgtcagg tgagcgtcat tatgtctggc gagctccagg gctttattca
    44701 ccagtaaggc atcttcttca ttcccggaaa ttgccacgcc aatgtgttta taagccatgt
    44761 ttaactcctt ctaaagcaac tccatcaagc taacgaacgc agtgataact caaaaataat
    44821 catctcggcc tggcatgaga aagtgaaggc cgcatcaagc tcaacttgcc cctccagatc
    44881 cttaatctta taggccacgc tggccccttc atctgcagag gatgcagcgg cggcaagctc
    44941 tttcagtttg gtcataaaag actctgcttc ttcgcgggtg gcaaaaaagc gcgaaaactt
    45001 actggtacag ttgtcgttat caatcaccgt accgatatca atcgcacatc ctttagtact
    45061 gcatttatct acgacatctt tcatagggca cctctgtata ttcgcctctc tgtcgattac
    45121 gacttaagaa taatgcgccg ttaagggaag aacaaacaag taatgatctt atggattacc
    45181 attttttttg atattgctta aggtgaagag ttcagaagga ttttccgggt attgtcattt
    45241 gcatcgtgga aataacaatc ccggaaaaaa atagcagtaa gagatatatt aaaggttaat
    45301 tacttgattt attgtcggct ttatacttca catcctgagt atctttacca tatttatttt
    45361 cgccttgtgt gccaacaaac gcaccaagat cgataagcat catcaccaga atcaacgtcg
    45421 ggacaaaacg ccccaccgcc cattgccaga cacccggtaa aatcgcccag ttacccgcca
    45481 gcagcatcca cgccacaatc atcagaaatg cccatgcgcc ggaacgcccg cgatcatgca
    45541 agcgcttaac agttactgcc gctgttggcc agagcaagca cacaaggcaa aacgccgcgg
    45601 tctgaatatc gagtagattc ttacccgcca gtgagaaaag caccagcatg cctgcgaacc
    45661 acaggcctat ccaaatccag aaatcacggc gtccaatacg ccctttaaat gagaataacc
    45721 attgctgtat ggtcatgtaa gttccttgat ggttgtcttt tcctggattc tacccttttg
    45781 acaagggcga cagatatcgt tttaatcgga gccagtcata acaaaaggta ctgtcaatga
    45841 agccagggtg tacgctgttt tttctcttat gttctgcatt aaccgttaca acaacggcgc
    45901 atgcacaaac accagatacg gcaacgaccg cgccttatct gctggctgga gcccctactt
    45961 tcgatctctc catcagccag tttcgagaag actttaacag ccagaatccc agcctgccac
    46021 tgaacgaatt tcgtgccatc gacagcagtc ccgacaaagc caatctcact cgtgctgcaa
    46081 gtaaaattaa cgagaacttg tatgcttcta cagcgctgga gcgcggtacc ttaaaaatca
    46141 aaagcattca aatgacctgg ctacccatcc aggggccaga gcaaaaagca gcgaaagcga
    46201 aagctcagga atacatggca gcagtgatcc gcacactcac cccattaatg accaaaacac
    46261 aaagccagaa aaaactgcag tcgctactaa cggcggggaa aaacaaacgt tattacaccg
    46321 agacagaagg tgcactgcgt tatgttgtcg cggacaacgg cgaaaagggg ctgaccttcg
    46381 ctgttgaacc gattaagctg gcgctatctg aatcgcttga aggtttgaat aaatgacaaa
    46441 aagcaaagcc tttgtgccga tgaatctcta tactgtttca cagacctgct gccctgcggg
    46501 gcggccatct tcctttattc gcttataagc gtggagaatt aaaatgcgac atcctttagt
    46561 gatgggtaac tggaaactga acggcagccg ccacatggtt cacgagctgg tttctaacct
    46621 gcgtaaagag ctggcaggtg ttgctggctg tgcggttgca atcgcaccac cggaaatgta
    46681 catcgatatg gcgaagcgcg aagctgaagg cagccacatc atgctgggtg cgcaaaacgt
    46741 ggacctgaac ctgtccggcg cattcaccgg tgaaacttct gccgctatgc tgaaagacat
    46801 cggcgctcag tacatcatca tcggccactc tgaacgtcgt acttaccaca aagagtctga
    46861 cgaactgatt gcgaaaaaat tcgcggtgct gaaagagcag ggtctgactc cggttctgtg
    46921 catcggtgaa accgaagctg aaaacgaagc gggaaaaact gaagaagttt gcgcacgtca
    46981 gatcgacgcg gtactgaaaa ctcagggtgc tgcggcattc gaaggtgcgg ttatcgctta
    47041 cgaacctgta tgggcaatcg gtactggcaa atctgcaact ccggctcagg cacaggctgt
    47101 tcacaaattc atccgtgacc acatcgctaa agttgacgct aacatcgctg aacaagtgat
    47161 cattcagtac ggcggctctg taaacgcgtc taacgctgca gaactgtttg ctcagccaga
    47221 catcgacggc gcgctggttg gcggtgcttc tctgaaagct gacgctttcg cagtgatcgt
    47281 taaagctgca gaagcggcta aacaggctta agtctgacag gtgccggatt tcatatccgg
    47341 cacttacttt ccttaactcc tcgccttaac gcaaaatctc acactcatga tcctggattt
    47401 cctcggctga agcacggttc agcgtcagta gatttcgttg tgtcgccagc aacacaaacg
    47461 aattatcact ctgccgtacc atcgccagcc cgtagcttcc catatgttcc cgcgcctcag
    47521 gcacttcttc tgcaagcatc ataaatgggc tgcgttgtac cagttcgctt tccgttaccc
    47581 gacgcgccaa gtattcatgc ccgcgcaaac cacctggcag tggcaaccag cggctgctga
    47641 tgttcgccag atttttatcc agttgttcgc gcacatcagg acgaatacaa gagatatgaa
    47701 tatgaaaatg gttttgcgta cgcccggtgc gggagttgat cgccaaagaa accgcgcgat
    47761 cgggaaccgg ctggccgtat tttttgctca taaaatcacg cgcctgccag gccaaccaaa
    47821 agaagttcgg cgttgaagga tcggtcaaca aaggactttc agtaccgtta atacgatacg
    47881 ttggcatcaa cagatattgc agtggaccat taagatcttt taaaaccacg tatccggcat
    47941 tgggtttgac ttccgcacat ggcgaaggat tttgattttg ctgctgattg ggcaaacatt
    48001 cctcaaggac aatcttacgt aatgtatccg actcttcacc ggttaatttc cagtaaccaa
    48061 taccggcagc cacaacggcg ataactatca tcaccaaaaa aagaagaccc gcttttttca
    48121 tctttttttc cctgtacctc aaagagatgc aagggtaacg caaaatcgtg acaaataaaa
    48181 aacccggtcg cgagaaacca ccgggtttga taattatcct agggaaatca gcgtttgctg
    48241 atctgatcga acgtaccgcc gttagcaaaa tgctcttttt gcgctttcgt ccagccgccg
    48301 aactcttcat caatggtgaa taacttcagc tttggaaacg cattttcgta ctttttcgct
    48361 acttcagcgt cgcgcggacg gtagtagttt ttcgcggcaa tttcctgacc ttctggcgag
    48421 tagagatatt tcaggtaggc ttccgccacc tctttggtgc cttttttctc gaccacttta
    48481 tcgaccaccg acaccgttgg ctctgcgagg atagactcac tcggcgtgac gatttcgaat
    48541 ttatctttcc ccagttcatt cgctgccagc agagcttcgt tttcccaggc gatcagtaca
    48601 tcgccaatcc cgcgctcgac aaaggtgtta gtggagccgc gcgcgccaga atccagaact
    48661 tcgacgtttt tatacagtgc ccgaacaaaa tcctgtgctt ttgcctgatc gttgttgttg
    48721 tgatgcagcg cgtagcccca ggcagccagg tagttccagc gtgcgccacc agagcttttc
    48781 ggattaggcg tgatcaccga aacacccggt ttaatcagat cgttccagtc atggatctgc
    48841 ttcggattgc ccttacgcac caggaaaaca atggtggaag tgtacggtgc ggagttatcc
    48901 ggcagacgtt tgatccactc tttatcaatc cgcccgcgtt ccgcaatcgc gtccacgtca
    48961 taggccagag ccagcgtgac aacatcagct tcaataccgt tgattaccga cgtcgcttgt
    49021 ttacctgaac caccgtgtga ctgacgaatc accacgttat caccagtttg ctgtttccag
    49081 tgggcgctga atgccttgtt gtactgttcg tacaattcgc gcgttggatc atatgaaacg
    49141 ttaagaagct gaatatcctt tgccataacg ctggttgccg ccagcaaaaa tgttaaccct
    49201 acgccccact tgttcatcgc ccgactctct tatgttgtgt tgtgatgagc aaagcgtgcc
    49261 agaaggttaa ccaaacatta aagaataaaa aaagattggc tataacttgc gggtatatgt
    49321 tgagggatta aaaaggcgga agaaatccgc ctcatattgc tgacaaagtg cgctttgtcc
    49381 atgccggatg cggcgcgaac gccttatccg gcctacaaaa gtttgcaaat tcaataaatt
    49441 gcagaattca tgtaggcctg ataagcgaag cgcatcaggc atttttgctt ctgtcatcgg
    49501 tttcaggcta aaggaatctg cctttttccg aaatcattaa tacagttttt tcgcgcagtc
    49561 cagccagtca cctttgaacg gacgcttcat gttttcgata gcgtcgatga tgtcgtggtg
    49621 aaccagctgt tcgttctgga tacctacaca acgaccgccg taacctgcca acagcagatc
    49681 gatagcgtaa gcgcccatac gggaagccag aatacggtcg taaggcaccg gagaaccacc
    49741 gcgctggatg tggcccagca cagttgcgcg ggtttcacga ccggtttctt tctcgatgaa
    49801 atgcgccagt tcgtcaacat cacacatatg ttcggtaatc gccacgatcg cgtgtttttt
    49861 acctttcgcg atacccgctt tgatttcgtt taccaggtct tcacggctga attcaacttc
    49921 cggaacgaca acgaattcac agccaccggc aatggccgca gccaaggtca gatcgccaca
    49981 ataacggccc atcacttcca ccacggaaat acgctggtga gaagaagagg tgtcacgcag
    50041 acggtcgatc gcttctacaa cggtgctcag cgcagtgaag aaaccgatag tgtagtcagt
    50101 gcctttgatg tcgttgtcga tagtgcccgg cagaccgatg cacgggaagc ccatttcggt
    50161 cagacgcatt gcacccatgt aggaaccgtc accgccgata accaccagcg cgtcgatacc
    50221 acgttttttc aggttttcga tagccacggc gcggatgttc tcgtcgcgga attccgggaa
    50281 acgcgcagaa ccgaggaacg taccgccacg gttgatcatg tcagacacgc tgtaacggtc
    50341 tagctgtacc atacggtctt catacagacc cagatagccg tcataaatac ccattacttc
    50401 cagaccttct gtcagcgcag aacgaacaac cccgcgaatt gcggcgttca tgcctggcgc
    50461 atcaccgccg cttgtcaaca cacctatttt cttaatcatg actacctctg aactttggaa
    50521 tgcaaaatga aatctgttgc cggaagtctt cttgcacatc gaagtgatcc aacgaatgtg
    50581 caaatagtat aacaatcact tcctgctgaa ttgattcagg tcaggccaaa tggcggtatt
    50641 ttatacacaa aatgcgggtc tggctctctt ttatactgat tatgaaagca tagaccgttt
    50701 accctccctg ggtacgacgg aacaggggtc ctgatggata atcacatccg atcccggaaa
    50761 acgccgtaaa atagcctgct ctacctgatc cgccaccata tgtgcctgaa ccaaaggcag
    50821 agagtcttcc atttccaaat gaatctgaat aaagcgggtc ggccctgact gccgcgtgcg
    50881 aagatcgtga gcgccgctaa cacccggcca ggaagtcacg atatcaataa tttcttgccg
    50941 ttcttcatca ggcaatgcgc gatccagtaa tgactgtacc gcctcatatc ccatgcgtaa
    51001 cgcgctatat aaaatataga tgccgattcc caatgcaaac agagcatcgg cgcgatgcca
    51061 gccgtaccaa gacaacccca gcgccagcag aattgcgccg ttcatcataa catcagactg
    51121 gtaatgtagc atatcagccc gcaccgcctg gctttgcgtc cggcgcacca cccaccgctg
    51181 aaacgagaca aggataatcg tacaaattag cgccacaatt gtcacgataa ccccgacgcc
    51241 tggatctgtc atcggtgttg gagataccag atgttgaata cccgtcaaaa acaggaatag
    51301 tgccgaaccg gagataaaca tactttgcgc cagcgccgcg agggactcag ctttaccgtg
    51361 accaaacgag tgattatcgt cggcaggttg cagggaatat cgcaccacca gtaaattcgt
    51421 caacgacgcg ccgatatcca ccagcgaatc caccagcgcg gcgagaatac tcaccgaccc
    51481 ggtataccac catgccaaaa ttttaatcag caatagcaac gaagccatcg ccgtcgcagc
    51541 aatcgccgcc cgactgacca gccgtccata agattgattc ataaatactc ccgctatcaa
    51601 ctgacgctag tataacggaa gcaaatcatc tgcaatgcat taagcagcag gcaaattgag
    51661 gataaaaaaa aaccccacat catgtggggg aagacaggga tggtgtctat ggcaaggaaa
    51721 acagggttta ctactgggaa cgtgagttgc tactactcaa tagcttcaac gatgaacttt
    51781 tttgccattg cgtcacgtca cgcaactgct ccattcgttg ttgatgtttc tcgtttaaaa
    51841 ccgcttgctg ctccggcgtt aacaggcgat acatttggtt gcggactttt gccatctcaa
    51901 cctgacgagc aatttgctca ttcgccattt tttctgcctg tgcgcgcaca gcgttttcat
    51961 caaaattttc tgcggtgaca aggcgatgca ttgtctccag ttcgctaaca ttaacaggag
    52021 gctgtttgtg ccgggcctgt tgcataagat ctcgcatctg ctgacgctga tgttcggtta
    52081 aacttatgcc gtcgaacata tggctctgcg tactgcgctg cgtaagttct tcacccggat
    52141 gccagttatc gcctgaaccg acttcagcag cgtggcttaa tgaactgact gccagcgttg
    52201 aggccatgac ggcagcggta actatgcgca tcatttgctc ccaaaatctt tctgtcgcga
    52261 ttcaacgata gagagtttac gattcaggct gcaaacatgc gtcagggggt gtaaaacaac
    52321 gtaaagtcat ggattagcga cgtctgatga cgtaatttct gcctcggagg tatttaaaca
    52381 atgaataaaa tcctgttagt tgatgatgac cgagagctga cttccctatt aaaggagctg
    52441 ctcgagatgg aaggcttcaa cgtgattgtt gcccacgatg gggaacaggc gcttgatctt
    52501 ctggacgaca gcattgattt acttttgctt gacgtaatga tgccgaagaa aaatggtatc
    52561 gacacattaa aagcacttcg ccagacacac cagacgcctg tcattatgtt gacggcgcgc
    52621 ggcagtgaac ttgatcgcgt tctcggcctt gagctgggcg cagatgacta tctcccgaaa
    52681 ccgtttaatg atcgtgagct ggtggcacgt attcgcgcga tcctgcgccg ttcgcactgg
    52741 agcgagcaac agcaaaacaa cgacaacggt tcaccgacac tggaagttga tgccttagtg
    52801 ctgaatccag gccgtcagga agccagcttc gacgggcaaa cgctggagtt aaccggtact
    52861 gagtttaccc tgctctattt gctggcacag catctgggtc aggtggtttc ccgtgaacat
    52921 ttaagccagg aagtgttggg caaacgcctg acgcctttcg accgcgctat tgatatgcac
    52981 atttccaacc tgcgtcgtaa actgccggat cgtaaagatg gtcacccgtg gtttaaaacc
    53041 ttgcgtggtc gcggctatct gatggtttct gcttcatgat aggcagctta accgcgcgca
    53101 tcttcgccat cttctggctg acgctggcgc tggtgttgat gttggttttg atgttaccca
    53161 agctcgattc acgccagatg accgagcttc tggatagcga acagcgtcag gggctgatga
    53221 ttgagcagca tgtcgaagcg gagctggcga acgatccgcc caacgattta atgtggtggc
    53281 ggcgtctgtt ccgggcgatt gataagtggg caccgccagg acagcgtttg ttattggtga
    53341 ccaccgaagg ccgcgtgatc ggcgctgaac gcagcgaaat gcagatcatt cgtaacttta
    53401 ttggtcaggc cgataacgcc gatcatccgc agaagaaaaa gtatggccgc gtggaactgg
    53461 tcggtccgtt ctccgtgcgt gatggcgaag ataattacca actttatctg attcgtccgg
    53521 ccagcagttc tcaatccgat ttcattaact tactgtttga ccgcccgcta ttactgctga
    53581 ttgtcaccat gttggtcagt acgccgctgc tgttgtggtt ggcctggagt ctggcaaaac
    53641 cggcgcgtaa gctgaaaaac gctgccgatg aagttgccca gggaaactta cgccagcacc
    53701 cggaactgga agcggggcca caggaattcc ttgccgcagg tgccagtttt aaccagatgg
    53761 tcaccgcgct ggagcgcatg atgacctctc agcagcgtct gctttctgat atctctcacg
    53821 agctgcgcac cccgctgacg cgtctgcaac tgggtacggc gttactgcgc cgtcgtagcg
    53881 gtgaaagcaa ggaactcgag cgtattgaaa ccgaagcgca acgtctggac agcatgatca
    53941 acgatctgtt ggtgatgtca cgtaatcagc aaaaaaacgc gctggttagc gaaaccatca
    54001 aagccaacca gttgtggagt gaagtgctgg ataacgcggc gttcgaagcc gagcaaatgg
    54061 gcaagtcgtt gacagttaac ttcccgcctg ggccgtggcc gctgtacggc aatccaaacg
    54121 ccctggaaag tgcgctggaa aacattgttc gtaatgctct gcgttattcc catacgaaga
    54181 ttgaagtggg ctttgcggta gataaagacg gtatcaccat tacggtggac gacgatggtc
    54241 ctggcgttag cccggaagat cgcgaacaga ttttccgtcc gttctatcgg accgatgaag
    54301 cacgcgatcg tgaatctggc ggtacaggat tggggctggc gattgttgaa accgccattc
    54361 agcagcatcg tggctgggtg aaggcagaag acagcccgct gggcggttta cggctggtga
    54421 tttggttgcc gctgtataag cggagttaaa ctccgcattt gtaggcagga taaggcgttt
    54481 acgccgcatc cggcatttga gcaggatgcc tgatgcgacg ctgatagcgt cttatcaggc
    54541 ctacactccg ggggttattt tccccacaac cggcgggaaa aatcttcgat cttgccgctc
    54601 agtcggcgct tttgcatcgt ccgcgtccag cttttcgata acccggcggc ggagagtaaa
    54661 cggtgatact gatcgtcatc aaacggcata tgccatgcga tggcggcagc ctcctgcacg
    54721 gtcacatcgc tgacacggga aaccaactcc agcggcgcgt ccgcagacac tttccctagc
    54781 gctatcacgc tatacaacca ccccacctta cctgtgtttt gcatcaactg cgctatatcg
    54841 ctgatatcaa aatggtaatt gagtttatag caaggcgagc gcggctggct gacctgaatt
    54901 aatgcctctc cccagcggaa aatatcgccc atatgaacat tactttccgt gagaccgtcg
    54961 gttgagagat tttcgccaaa cgcaggcgca acaaacaact ccgcctgttc cggaaattcc
    55021 cgcacccagt agagatagtg ctcacgagga taatgacaca gcgctctgtc tggcccgccg
    55081 tgaacctttt tctccgcctg ctcgtcacct tccagcccca gctccgtcag catcaactca
    55141 ccatcaacct ggattttggc aattgcgctg ggtttgcctt cgggataagc ctgaatcttg
    55201 cctgtgtata catcaaccgg atatcgcatc tgttccctct ccacgagaaa tttccccatt
    55261 agaccatatt tattttatgt cagccgtgct gttttcgcgg taagtatcca ggcaggaact
    55321 caatagcaaa ccggacgaac gttagttcat ccggctgtgg ggattaagga agcaggttta
    55381 tttcaccgta cctaaaattt cgattttcgc tgctctggct ttgactttac gtgaccagat
    55441 agaggtaaga attggcacca gaatcgaagt cacaatgacc gccgtcgcta ccagcgaagt
    55501 tgctgccgga gccatcggtt taaacgcagg caccatttct gcaatcagca caggtgtcgc
    55561 taccgcggcc cctgcggaac tggaagcggc aattccggca gtgccatcgc caccgccaat
    55621 caatttatcg gcgataatca acggaatacc ggtcacgata attactgcca cacccaacag
    55681 gatccccagc aaaccagtct gggcaatcac agtcaaatca atggtattgc ccagcgcgaa
    55741 ggcaaagaat ggaatcagcg tttgcaccgc tttgctgaaa aattctcgta attcagggtc
    55801 aagattcccc agggcaaagc ccaccaggaa cggtaatact gcgccgacga aaacatgcgg
    55861 ttcaaacgag gcaatcccgg cagtgcccag aataatcatc gtcatgagcg gcccggactc
    55921 caacgacatc aacacaaatg ccccagcttc ttctttcgta ccgtactgct gcatgatgga
    55981 agcgtaaagt ccgccgttgg tcatatccat cgccgccacc agcgccagcg ttgaaagtcc
    56041 ggcaaagaat ccaacttcaa caccatgttc cggaataatg cgcgaggcaa ttgccgcaac
    56101 cacccacgcg acggcaattt tagttaccac cagcgtaccg gatttacgca gtaccgttcc
    56161 cgtcgcgctt aattttattg acgcccccat gcaaaaaaac cacaccgcca gaatgggcac
    56221 cgtaccggta atcatcccgt tggtgaatga tccaaaatat ttccccgccc ccggcgagaa
    56281 ggtgtggcac agtgcgccaa ggaatagcgg gacgagcatc atcccccccg ggattttctc
    56341 aatcgagcgt tttatctgca tttccatcac ctgtcggcat atttttaagg gctgatgcgt
    56401 aattcctcaa tggagcatca gtgttgatgg aaacgaagat agacattcat ttaatcaata
    56461 aaagtgatca aaaccacatc aattgaaacg ctgttttatt tttataggat cattgttttt
    56521 attttggaat tgatcacaaa aaaaccaccg ccgttggcga tggttcattt tttaagctga
    56581 tatgcggcct atacgcctca ttgcagcagg cggcaaatga ttattttttc gccgcaaaac
    56641 gtgccgctgc ttcgtcccag ttcaccacgt tccagaactc tttgatgtag tccggacggc
    56701 ggttctggaa tttcaggtag taagcatgtt cccacacatc caggcccaga atcgggaagc
    56761 cggaagcgcc agaaatagct tcacccatca gcggagaatc ctggttagca gtagaaacca
    56821 ccgccagttt atcgcctttc agcaccagcc atgcccagcc ggaaccaaag cgggaagctg
    56881 ccgctttttc aaattctgct ttgaagttat caacggagcc gaagtcacgt tcgatagccg
    56941 ctttcaggtc accctgcagg gtggtgcctt ttttcagacc tttccagaac aggctgtggt
    57001 tagcgtgacc gccagcgttg ttgcgcagta cggttttctt gtctgctggc agctggtcca
    57061 gtttggtgat cagctcttca accggcaggt tggcaaattc tggcaggctt tccagcgccg
    57121 cgttggcgtt gtttacgtag gtctgatggt gtttggtgtg gtggatttcc atggtctgct
    57181 tatcgaagtg cggttccagg gcatcgtaag catacggcag ggatggcagg gtatagctca
    57241 tattcatctc cagtattgtc gggcggccga ttgttaatgc cgcgtaagca gttggttcat
    57301 tatagttaat taaatgatat tgaaaatgat tatcaatgcc gtacttttcg taagggtatg
    57361 gttttgcagg aaaatgcccg agatgtgaag caaatcaccc acttaatgcc gtgattgcca
    57421 gtaaatcgac aacggcggca acaggcgaaa ggttaatcga cagcacgatt tttacactca
    57481 tctcgtcgga gatgtgaagc gacgaaaaat gatgaggata agaagatgag taacgcgatt
    57541 acgatgggga tattttggca tttgatcggc gcggccagtg cagcctgttt ttacgctccg
    57601 ttcaaaaaag taaaaaaatg gtcatgggaa accatgtggt cagtcggtgg gattgtttcg
    57661 tggattattc tgccgtgggc catcagcgcc ctgttactac cggatttctg ggcgtattac
    57721 agctcgttta gtctctctac gctgctgcct gtttttctgt tcggcgctat gtggaggatc
    57781 ggtaatatca actatggcct gaccatgcgt tatctcggca tgtcgatggg aattggcatc
    57841 gccattggca ttacgttgat tgtcggtacg ctgatgacgc caattatcaa cggcaatttc
    57901 gatgtgctga ttaacaccga aggcggacgc atgacgttgc tcggcgttct ggtggcgctg
    57961 attggcgtag ggattgtaac tcgagccggg cagttgaaag agcgcaagat gggcattaaa
    58021 gccgaagagt tcaatctgaa aaaagggctg gtgctggcgg tgatgtgcgg cattttctct
    58081 gccgggatgt cctttgcgat gaacgccgca aaaccgatgc atgaagccgc tgccgcactt
    58141 ggcgtcgatc cactgtatgt cgctctgcca agctatgttg tcatcatggg cggcggcgcg
    58201 atcattaacc tcggtttctg ttttattcgt ctggcaaaag tgaaggattt gtcgctaaaa
    58261 gccgacttct cgctggcaaa accgctgatc attcacaatg tgttactctc ggcactgggc
    58321 gggttgatgt ggtatctgca attctttttc tatgcctagg gccacgcccg cattccggcg
    58381 cagtatgact acatcagttg gatgctgcat atgagtttct atgtattgtg cggcggtatc
    58441 gtcgggctgg tgctgaaaga gtggaacaat acaggacgcc gtccggtaac ggtgttgagc
    58501 ctcggttgtg tggtgattat tgtcgccgcc aacatcgtcg gcatcggcat ggcgaattaa
    58561 tctttctgcg aattgagatg acgccactgg ctgggcgtca tcccggtttc ccgggtaaac
    58621 accaccgaaa aatagttact atcttcaaag ccacattcgg tcgaaatatc actgattaac
    58681 aggcggctat gctggagaag atattgcgca tgacacactc tgacctgccg cagatattga
    58741 ttgatggtca ttccagtctg ctggcgaaat tgctgacgca aaacgcgctc actgcacgat
    58801 gcctcatcac aaaatttatc cagcgcaaag ggacttttca ggctagccgc cagccgggta
    58861 atcagcttat ccagcaacgt ttcgctggat gttggtggca acgaatcact ggtgtaacga
    58921 tggcgattca gcaacatcac caactgcccg aacagcaact cagccatttc gttagcaaac
    58981 ggcacatgct gactactttc atgctcaagc tgaccgataa cctgccgcgc ctgcgccatc
    59041 cccacgctac ctaagcgcca gtgtggttgc cctgcgctgg cgctaaatcc cggaatcgct
    59101 ccctgccagt caagattcag cttcagccgc tccgggcaat aaataatatt ctgcaaaacc
    59161 agatcgttaa cggaagcgta ggagtgttta tcgtcagcat gaatataaaa gagatcgcca
    59221 cgggtaatgc gataaggacg atcgttgagt acatgcagac cattaccgcg ccagacaatc
    59281 accagctcac aaaaatcatg tgtatgttcg gcaaagacat cttgcggata acggtcagcc
    59341 acagcgactg cctgctggtc gctggcaaaa aaatcatctt tgagaagttt taactgatgc
    59401 gccaccgtgg ctacctcggc cagagaacga agttgattat tcgcaatatg gcgtacaaat
    59461 acgttgagaa gattcgcgtt attgcagaaa gccatcccgt ccctggcgaa tatcacgcgg
    59521 tgaccagtta aactctcggc gaaaaagcgt cgaaaagtgg ttactgtcgc tgaatccaca
    59581 gcgataggcg atgtcagtga cgctggcctc gctgtggcgt agcagatgtc gggctttcat
    59641 cagtcgcagg cggttcaggt atcgctgagg cgtcagtccc gtttgctgct taagctgccg
    59701 atgtagcgta cgcagtgaaa gagaaaattg atccgccacg gcatcccaat tcacctcatc
    59761 ggcaaaatgg tcctccagcc aggccagaag caagttgaga cgtgatgcgc tgttttccag
    59821 gttctcctgc aaactgcttt tacgcagcaa gagcagtaat tgcataaaca agatctcgcg
    59881 actggcggtc gagggtaaat cattttcccc ttcctgctgt tccatctgtg caaccagctg
    59941 tcgcacctgc tgcaatacgc tgtggttaac gcgccagtga gacggatact gcccatccag
    60001 ctcttgtggc agcaactgat tcagcccggc gagaaactga aatcgatccg gcgagcgata
    60061 cagcacattg gtcagacaca gattatcggt atgttcatac agatgccgat catgatcgcg
    60121 tacgaaacag accgtgccac cggtgatggt atagggctgc ccattaaaca catgaatacc
    60181 cgtgccatgt tcgacaatca caatttcatg aaaatcatga tgatgttcag gaaaatccgc
    60241 ctgcgggagc cggagttcta tcgccacgga cgcgttacaa gacggaaaaa aatccacact
    60301 atgtaatacg gtcatactgg cctcctgatg tcgtcaacac ggcgaaatag taatcacgag
    60361 gtcaggttct taccttaaat tttcgacgga aaaccacgta aaaaacgtcg atttttcaag
    60421 atacagcgtg aattttcagg aaatgcggtg cgcatcacat caccacaatt cagcaaattg
    60481 tgaacatcat cacgttcatc tttccctggt tgccaatggc ccattttcct gtcagtaacg
    60541 agaaggtcgc gaattcaggc gctttttaga ctggtcgtaa tgaaattcag caggatcaca
    60601 ttatgacctt tcgcaattgt gtcgccgtcg atctcggcgc atccagtggg cgcgtgatgc
    60661 tggcgcgtta cgagcgtgaa tgccgcagcc tgacgctgcg cgaaatccat cgttttaaca
    60721 atgggctgca tagccaaaac ggttatgtca cctgggatgt ggatagcctg gaaagtgcca
    60781 ttcgccttgg attaaacaag gtgtgcgagg aagggattcg tatcgatagc attgggattg
    60841 atacctgggg cgtggacttt gtgctgctcg accaacaggg tcagcgtgtg ggcctgcccg
    60901 ttgcttatcg cgatagccgc accaatggcc tgatgccgca ggcacaacag caactcggca
    60961 aacgcgatat ttatcagcgt agcggcatcc agtttctacc cttcaatacg ctttatcagt
    61021 tgcgtgcgct gacggagcaa caacctgaac ttattccaca cattgctcac gctctgctga
    61081 tgccggatta cttcagttat cgcctgaccg gcaagatgaa ctgggaatat accaatgcca
    61141 ccaccacaca actggtcaat atcaatagcg acgactggga cgagtcgcta ctggcgtgga
    61201 gcggggccaa caaagcctgg tttggtcgcc cgacgcatcc gggtaatgtc attgggcact
    61261 ggatttgccc acagggtaat gagattccgg tggtcgccgt tgccagccat gataccgcca
    61321 gcgcggttat cgcctcgccg ttaaacggtt cacgcgccgc ttatctctct tctggcacct
    61381 ggtcattgat gggcttcgaa agccagacgc catttaccaa tgacacggcg ctggcagcca
    61441 acatcaccaa tgaaggcggg gcggaaggtc gctatcgggt gctgaaaaat attatgggct
    61501 tatggctgct tcagcgagtg cttcaggaac ggcaaatcaa cgatctcccg gcgcttatcg
    61561 ccgcgacaca ggcacttccg gcctgccgct tcatcatcaa tcccaatgac gatcgcttta
    61621 ttaatcctga cgagatgtgc agcgaaattc aggctgcgtg tcgggaaacg gcgcaaccga
    61681 tcccgggaag tgatgctgaa ctggcgcgct gtattttcga cagtctggcg ctgctgtatg
    61741 ccgatgtgtt gcatgagctg gcgcagctgc gcggtgaaga tttctcgcaa ctgcatattg
    61801 tcggcggcgg ctgccagaac acgctgctca accagctatg cgccgatgcc tgcggtattc
    61861 gggtgatcgc cgggcctgtt gaagcctcaa cgctcggcaa tatcggcatc cagttaatga
    61921 cgctggatga actcaacaat gtggatgatt tccgtcaggt cgtcagcacc accgcgaatc
    61981 tgaccacctt tacccctaat cctgacagtg aaattgccca ctatgtggcg cagattcact
    62041 ctacacgaca gacaaaggag ctttgcgcat gaccactcaa ctggaacagg cctgggagct
    62101 ggcgaaacag cgtttcgcgg cggtggggat tgatgtcgag gaggcgctgc gccaacttga
    62161 tcgtttaccc gtttcaatgc actgctggca gggcgatgat gtttccggtt ttgaaaaccc
    62221 ggaaggttcg ctgaccgggg ggattcaggc cacaggcaat tatccgggca aagcgcgtaa
    62281 tgccagtgag ctacgtgccg atctggaaca ggctatgcgg ctgattccgg ggccgaaacg
    62341 gcttaattta catgccatct atctggaatc agatacgcca gtctcgcgcg accagatcaa
    62401 accagagcac ttcaaaaact gggttgaatg ggcgaaagcc aatcagctcg gtctggattt
    62461 taacccctcc tgcttttcgc atccgctaag cgccgatggc tttacgctct cgcatcccga
    62521 cgacagcatt cgccagttct ggattgatca ctgcaaagcc agccgtcgcg tttcggccta
    62581 ttttggcgag caactcggca caccatcggt gatgaacatc tggatcccgg atggtatgaa
    62641 agatatcacc gttgaccgtc tcgccccgcg tcagcgtctg ctggcagcac tggatgaggt
    62701 gatcagcgag aagctaaacc ctgcgcacca tatcgacgcc gttgagagca aattgtttgg
    62761 cattggcgca gagagctaca cggttggctc caatgagttt tacatggggt atgccaccag
    62821 ccgccagact gcgctgtgcc tggacgccgg gcacttccac ccgactgaag tgatttccga
    62881 caagatttcc gccgccatgc tgtatgtgcc gcagttgctg ctgcacgtca gccgtccggt
    62941 tcgctgggac agcgatcacg tagtgctgct ggatgatgaa acccaggcaa ttgccagtga
    63001 gattgtgcgt cacgatctgt ttgaccgggt gcatatcggc cttgacttct tcgatgcctc
    63061 tatcaaccgc attgccgcgt gggtcattgg tacacgcaat atgaaaaaag ccctgctgcg
    63121 tgcgttgctg gaacctaccg ctgagctgcg caagctggaa gcggcgggcg attacactgc
    63181 gcgtctggca ctgctggaag agcagaaatc gttgccgtgg caggcggtct gggaaatgta
    63241 ttgccaacgt cacgatacgc cagcaggtag cgaatggctg gagagcgtgc gggcttatga
    63301 gaaagcgatt ttgagccagc gtgggtaaac actgccggat gcggcacgag cgccttatcc
    63361 ggcctacggg tcgaatgtcc atgcgggctg tcaccctcac cccggccctc tccctgaaag
    63421 ggcgagggag aaaaacatag cctcgtgcgg caacggagct gaacaccgct ccggacagtc
    63481 ccctcgcccc tacggggaga gggttagggt gaggggaaca agccgcacca tgtctaaaca
    63541 aggcaaccta atttacagga acacagaaca tgcaaaacat tacccagtcc tggtttgtcc
    63601 agggaatgat caaagccacc accgatgcct ggctgaaagg ctgggacgag cgcaacggcg
    63661 gcaacctgac gctgcgcctg gatgacgccg atatcgcgct ttatcacgac aacttccacc
    63721 cacaaccgcg ctatattccg ctcagccaac ccatgccttt actggcaaat acgccgttta
    63781 ttgtcaccgg ctccggcaaa ttcttccgta acgtccagct cgatcctgca gctaacttag
    63841 gcgtcgtaaa agtcgacagc gatggcgcgg gctaccatat tctctggggg ttaaccaacg
    63901 aagccgttcc aacttctgaa cttccggctc actttctctc ccactgcgag cgcattaaag
    63961 ccaccaacgg taaagatcgg gtgatcatgc actgccacgc caccaacctg atcgccctca
    64021 cctatgtact ggaaaacgac accgcggtct ttactcgcca actgtgggaa ggcagcaccg
    64081 aatgtctggt agtattcccg gatggcgttg gcattctgcc gtggatggtg cccggcacgg
    64141 acgaaatcgg ccaggcgacc gcacaagaga tgcaaaaaca ttcgctggtg ttgtggccct
    64201 tccacggtgt gttcggttcc ggaccgacgc tggatgaagc ctttggttta atcgacaccg
    64261 cagaaaaatc agcacaagta ttagtgaagg tttattcgat gggcggcatg aaacagacca
    64321 tcagccgtga agagttgata gcgctcggca agcgcttcgc cgttacacca ctagccagtg
    64381 cgctggcgct gtaaggagca aacatgatcc gcaaagcctt tgtcatgcag gtaaaccccg
    64441 acgcccacga agagtatcag cgtcggcata atcccatctg gccggaactg gaagcagtgt
    64501 tgaaatctca cggtgtgcat aactacgcca tttacctcga caaagcgcgt aatctgctgt
    64561 ttgccatggt agagattgaa tctgaagaac ggtggaatgc ggtagccagt actgatgttt
    64621 gccaacgttg gtggaaatat atgaccgatg ttatgcccgc taacccagat aacagcccgg
    64681 tgagtagcga gctgcaagaa gtgttttacc tgccgtaatt gcccaatgtg ccggagatgc
    64741 ctccggcaca atattttaaa tttcgatttg aaaaattaaa catattacat ctcccggtaa
    64801 gttatatttc cctgatacat tgtgagtaaa tcacaaaaat aatgaataac ccattaatga
    64861 ttcatgtggt ttatttaaat aaccattatg tgcattactc cgcaaatctg acctttcact
    64921 ctgtctcaaa ttgtcattgt ttaccgacat atcgcctctc atc
//
LOCUS       DADLSA010000005        63890 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.005, whole genome shotgun sequence.
ACCESSION   DADLSA010000005 DADLSA010000000
VERSION     DADLSA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.005"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..2565
                     /locus_tag="JN691_000334"
     CDS             <1..2565
                     /locus_tag="JN691_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HAY7241869.1"
                     /translation="SGGVPSFIGGLVVFVSAAFNAQAETWFDPAFFKDDPSMVADLSR
                     FEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTESLDAMGVNTDAF
                     PAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNARGYVPPERWDEGI
                     NALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNSTWSRSSGQTAEW
                     KNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNMLPDSLKGFAPVV
                     RGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVEIKEADGSVNSYS
                     VPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGPWGTTWYGGGQYA
                     EYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLYAKTLNQLGTNFQ
                     LMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFYTKRGKLQVNISQ
                     QLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNYSKSRGQPDADQV
                     FALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLGLTETLLDDGNLS
                     YSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGSLVAHSQG
                     ITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRIALDAASL
                     KRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRFGAIATLDGIQTN
                     SGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINSAITRMDAICR"
     gene            2556..3626
                     /locus_tag="JN691_000335"
     CDS             2556..3626
                     /locus_tag="JN691_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7241870.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLEARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            3638..4180
                     /locus_tag="JN691_000336"
     CDS             3638..4180
                     /locus_tag="JN691_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7241871.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4188..4703
                     /locus_tag="JN691_000337"
     CDS             4188..4703
                     /locus_tag="JN691_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7241872.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4669..5406
                     /locus_tag="JN691_000338"
     CDS             4669..5406
                     /locus_tag="JN691_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAY7241873.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5517..6527
                     /gene="pyrD"
                     /locus_tag="JN691_000339"
     CDS             5517..6527
                     /gene="pyrD"
                     /locus_tag="JN691_000339"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAY7241874.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6701..7243
                     /gene="zapC"
                     /locus_tag="JN691_000340"
     CDS             6701..7243
                     /gene="zapC"
                     /locus_tag="JN691_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAY7241875.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7240..8349)
                     /gene="ycbX"
                     /locus_tag="JN691_000341"
     CDS             complement(7240..8349)
                     /gene="ycbX"
                     /locus_tag="JN691_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAY7241876.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8593..10701
                     /gene="rlmKL"
                     /locus_tag="JN691_000342"
     CDS             8593..10701
                     /gene="rlmKL"
                     /locus_tag="JN691_000342"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAY7241877.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWAVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10713..12620
                     /locus_tag="JN691_000343"
                     /pseudo
     CDS             10713..12620
                     /locus_tag="JN691_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            12750..14003
                     /gene="pqiA"
                     /locus_tag="JN691_000344"
     CDS             12750..14003
                     /gene="pqiA"
                     /locus_tag="JN691_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAY7241878.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLMTILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGIGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14008..15648
                     /gene="pqiB"
                     /locus_tag="JN691_000345"
     CDS             14008..15648
                     /gene="pqiB"
                     /locus_tag="JN691_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAY7241879.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15645..16208
                     /gene="pqiC"
                     /locus_tag="JN691_000346"
     CDS             15645..16208
                     /gene="pqiC"
                     /locus_tag="JN691_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAY7241880.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16464..16631
                     /gene="rmf"
                     /locus_tag="JN691_000347"
     CDS             16464..16631
                     /gene="rmf"
                     /locus_tag="JN691_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAY7241881.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16701..17219)
                     /gene="fabA"
                     /locus_tag="JN691_000348"
     CDS             complement(16701..17219)
                     /gene="fabA"
                     /locus_tag="JN691_000348"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAY7241882.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17288..19048)
                     /locus_tag="JN691_000349"
     CDS             complement(17288..19048)
                     /locus_tag="JN691_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAY7241883.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19233..19685
                     /gene="matP"
                     /locus_tag="JN691_000350"
     CDS             19233..19685
                     /gene="matP"
                     /locus_tag="JN691_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAY7241884.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19761..20807)
                     /gene="ompA"
                     /locus_tag="JN691_000351"
     CDS             complement(19761..20807)
                     /gene="ompA"
                     /locus_tag="JN691_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAY7241885.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPSGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21163..21672)
                     /gene="sulA"
                     /locus_tag="JN691_000352"
     CDS             complement(21163..21672)
                     /gene="sulA"
                     /locus_tag="JN691_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAY7241886.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            21891..22520
                     /gene="sxy"
                     /locus_tag="JN691_000353"
     CDS             21891..22520
                     /gene="sxy"
                     /locus_tag="JN691_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAY7241887.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22483..24645)
                     /gene="yccS"
                     /locus_tag="JN691_000354"
     CDS             complement(22483..24645)
                     /gene="yccS"
                     /locus_tag="JN691_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAY7241888.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(24655..25101)
                     /locus_tag="JN691_000355"
     CDS             complement(24655..25101)
                     /locus_tag="JN691_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAY7241889.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25224..27278
                     /gene="helD"
                     /locus_tag="JN691_000356"
     CDS             25224..27278
                     /gene="helD"
                     /locus_tag="JN691_000356"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAY7241890.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27310..27768)
                     /gene="mgsA"
                     /locus_tag="JN691_000357"
     CDS             complement(27310..27768)
                     /gene="mgsA"
                     /locus_tag="JN691_000357"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAY7241891.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(27864..28526)
                     /locus_tag="JN691_000358"
     CDS             complement(27864..28526)
                     /locus_tag="JN691_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAY7241892.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            28699..29112
                     /locus_tag="JN691_000359"
     CDS             28699..29112
                     /locus_tag="JN691_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAY7241893.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29157..29474)
                     /gene="hspQ"
                     /locus_tag="JN691_000360"
     CDS             complement(29157..29474)
                     /gene="hspQ"
                     /locus_tag="JN691_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAY7241894.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(29532..30722)
                     /gene="rlmI"
                     /locus_tag="JN691_000361"
     CDS             complement(29532..30722)
                     /gene="rlmI"
                     /locus_tag="JN691_000361"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAY7241895.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            30817..31095
                     /gene="yccX"
                     /locus_tag="JN691_000362"
     CDS             30817..31095
                     /gene="yccX"
                     /locus_tag="JN691_000362"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAY7241896.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31092..31421)
                     /gene="tusE"
                     /locus_tag="JN691_000363"
     CDS             complement(31092..31421)
                     /gene="tusE"
                     /locus_tag="JN691_000363"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAY7241897.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31512..32171)
                     /gene="yccA"
                     /locus_tag="JN691_000364"
     CDS             complement(31512..32171)
                     /gene="yccA"
                     /locus_tag="JN691_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAY7241898.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32378..32465)
                     /locus_tag="JN691_000365"
     tRNA            complement(32378..32465)
                     /locus_tag="JN691_000365"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32429..32431),aa:Ser,seq:tga)
     gene            32891..34009
                     /gene="hyaA"
                     /locus_tag="JN691_000366"
     CDS             32891..34009
                     /gene="hyaA"
                     /locus_tag="JN691_000366"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAY7241899.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34006..35799
                     /gene="hyaB"
                     /locus_tag="JN691_000367"
     CDS             34006..35799
                     /gene="hyaB"
                     /locus_tag="JN691_000367"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HAY7241900.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALVIVQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            35818..36525
                     /gene="hyaC"
                     /locus_tag="JN691_000368"
     CDS             35818..36525
                     /gene="hyaC"
                     /locus_tag="JN691_000368"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HAY7241901.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            36522..37109
                     /locus_tag="JN691_000369"
     CDS             36522..37109
                     /locus_tag="JN691_000369"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAY7241902.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            37106..37504
                     /gene="hyaE"
                     /locus_tag="JN691_000370"
     CDS             37106..37504
                     /gene="hyaE"
                     /locus_tag="JN691_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAY7241903.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37501..38358
                     /locus_tag="JN691_000371"
     CDS             37501..38358
                     /locus_tag="JN691_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAY7241904.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38492..40036
                     /gene="appC"
                     /locus_tag="JN691_000372"
     CDS             38492..40036
                     /gene="appC"
                     /locus_tag="JN691_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAY7241905.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIVLVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40048..41184
                     /gene="appB"
                     /locus_tag="JN691_000373"
     CDS             40048..41184
                     /gene="appB"
                     /locus_tag="JN691_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY7241906.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVASNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFSAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41196..41288
                     /gene="cbdX"
                     /locus_tag="JN691_000374"
     CDS             41196..41288
                     /gene="cbdX"
                     /locus_tag="JN691_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAY7241907.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            41368..42666
                     /locus_tag="JN691_000375"
     CDS             41368..42666
                     /locus_tag="JN691_000375"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAY7241908.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(42781..44947)
                     /gene="etk"
                     /locus_tag="JN691_000376"
                     /pseudo
     CDS             complement(42781..44947)
                     /gene="etk"
                     /locus_tag="JN691_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(44967..45413)
                     /gene="etp"
                     /locus_tag="JN691_000377"
                     /gene_synonym="yccY"
     CDS             complement(44967..45413)
                     /gene="etp"
                     /locus_tag="JN691_000377"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HAY7241909.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(45401..46540)
                     /locus_tag="JN691_000378"
     CDS             complement(45401..46540)
                     /locus_tag="JN691_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY7241910.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(46586..48682)
                     /locus_tag="JN691_000379"
     CDS             complement(46586..48682)
                     /locus_tag="JN691_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY7241911.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSIGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(48682..49428)
                     /locus_tag="JN691_000380"
     CDS             complement(48682..49428)
                     /locus_tag="JN691_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY7241912.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(49425..50069)
                     /locus_tag="JN691_000381"
     CDS             complement(49425..50069)
                     /locus_tag="JN691_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY7241913.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(50176..50481)
                     /locus_tag="JN691_000382"
     CDS             complement(50176..50481)
                     /locus_tag="JN691_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7241914.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(50923..51135)
                     /gene="cspH"
                     /locus_tag="JN691_000383"
     CDS             complement(50923..51135)
                     /gene="cspH"
                     /locus_tag="JN691_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HAY7241915.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            51421..51633
                     /gene="cspG"
                     /locus_tag="JN691_000384"
     CDS             51421..51633
                     /gene="cspG"
                     /locus_tag="JN691_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HAY7241916.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            51644..51832
                     /locus_tag="JN691_000385"
     CDS             51644..51832
                     /locus_tag="JN691_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAY7241917.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            51807..52037
                     /gene="ymcE"
                     /locus_tag="JN691_000386"
     CDS             51807..52037
                     /gene="ymcE"
                     /locus_tag="JN691_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HAY7241918.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNIPYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            52027..52200
                     /locus_tag="JN691_000387"
     CDS             52027..52200
                     /locus_tag="JN691_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HAY7241919.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(52249..53322)
                     /locus_tag="JN691_000388"
     CDS             complement(52249..53322)
                     /locus_tag="JN691_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HAY7241920.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(53424..56090)
                     /gene="torS"
                     /locus_tag="JN691_000389"
                     /pseudo
     CDS             complement(53424..56090)
                     /gene="torS"
                     /locus_tag="JN691_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309175.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            56218..57246
                     /gene="torT"
                     /locus_tag="JN691_000390"
     CDS             56218..57246
                     /gene="torT"
                     /locus_tag="JN691_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HAY7241921.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGNPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(57219..57911)
                     /gene="torR"
                     /locus_tag="JN691_000391"
     CDS             complement(57219..57911)
                     /gene="torR"
                     /locus_tag="JN691_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HAY7241922.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            58041..59213
                     /gene="torC"
                     /locus_tag="JN691_000392"
     CDS             58041..59213
                     /gene="torC"
                     /locus_tag="JN691_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HAY7241923.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            59213..61759
                     /gene="torA"
                     /locus_tag="JN691_000393"
     CDS             59213..61759
                     /gene="torA"
                     /locus_tag="JN691_000393"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HAY7241924.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            61756..62355
                     /gene="torD"
                     /locus_tag="JN691_000394"
     CDS             61756..62355
                     /gene="torD"
                     /locus_tag="JN691_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HAY7241925.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIATLTVRDDARLELAADFCGLFLMTDKQVALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFAALCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(62448..62753)
                     /gene="cbpM"
                     /locus_tag="JN691_000395"
     CDS             complement(62448..62753)
                     /gene="cbpM"
                     /locus_tag="JN691_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAY7241926.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(62753..63673)
                     /gene="cbpA"
                     /locus_tag="JN691_000396"
     CDS             complement(62753..63673)
                     /gene="cbpA"
                     /locus_tag="JN691_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAY7241927.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGAPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT        15720 a        15825 c        16822 g        15523 t
ORIGIN      
        1 tctggtggag tgccatcgtt tattggtggg ctggtggtgt ttgtgtcggc agcgttcaat
       61 gcacaagctg aaacctggtt cgatcctgct tttttcaaag atgatccctc aatggtggcc
      121 gatttgtctc gtttcgaaaa aggacaaaaa ataacgccag gggtttatcg tgtcgatatt
      181 gttctgaatc agacaattgt agatacgcgc aacgtcaatt ttgttgagat aacgccagag
      241 aaggggattg ccgcctgttt gacgactgaa agcctggatg caatgggtgt taatactgat
      301 gcgtttccgg cttttaaaca actggacaaa caagcgtgtg tgccattggc ggagattatt
      361 ccggatgcca gcgtaacgtt taatgtgaat aaactccgtc tggaaatttc agtaccgcaa
      421 atcgccatca aaagtaatgc ccgtggttat gtcccccctg aacgttggga tgaagggatc
      481 aacgcgctat tactgggata ttcgtttagc ggggctaaca gtattcatag cagcgcagac
      541 agtgattctg gcgacagcta ttttctgaat ttaaacagtg gcgttaattt aggcccatgg
      601 agattgcgca acaattcaac atggagtcgc agtagtggcc aaaccgcaga atggaagaat
      661 ctcagcagct atttgcagcg ggcggttatt ccactaaaag gcgaactgac cgtaggtgat
      721 gattatactg caggcgattt tttcgatagt gtcagctttc gtggtgtgca gctggcgtca
      781 gatgacaaca tgctgccaga cagcctgaaa gggtttgcgc ctgtggtgcg tggtatcgcc
      841 aaaagcaatg cccagataac gattaagcaa aatggttaca ccatttacca aacttatgta
      901 tcgcctggtg cttttgaaat tagtgatctc tattccacgt cgtcgagcgg tgatttgtta
      961 gttgaaatca aagaagcgga cggcagcgtc aatagctaca gcgtaccgtt ttccagcgtg
     1021 ccattactcc agcgtcaggg gcgaatcaaa tacgcggtga cactggcgaa atacagaacc
     1081 aatagtaatg aacagcagga gagcaaattt gcccaggcca cgttgcagtg gggcggaccg
     1141 tggggaacga catggtatgg tggtggacaa tatgctgaat attaccgtgc cgccatgttt
     1201 ggtctgggat ttaaccttgg cgatttcgga gcaatttcgt tcgatgcgac ccaggcgaag
     1261 agtacgctgg cagaccaaag cgaacataaa ggtcagtcat atcgttttct gtatgccaaa
     1321 acgctcaacc aattgggcac taattttcaa ttgatgggct atcgctattc gacgtcgggt
     1381 ttctacaccc tttccgacac catgtataaa catatggatg gctacgaatt taatgacggt
     1441 gatgatgaag atacgccgat gtggtcgcgt tattacaatt tgttttacac caaacgtggc
     1501 aaactgcagg tcaatatctc ccagcaatta ggcgagtacg gttcgtttta tttaagtggt
     1561 agccagcaaa cttactggca taccgatcaa caggatcggc tattacagtt tggctacaac
     1621 acgcaaatta aagatctctc gctgggggtt tcctggaact acagtaagtc ccgtggtcaa
     1681 cctgatgctg atcaggtgtt tgcactaaat ttttccctgc cgctcaatct gttgctcccc
     1741 agaagtaatg atagctatac caggaaaaaa aattacgcct ggatgacctc taacaccagt
     1801 atcgataacg aagggcacat tacacaaaac ctgggtttaa cggagacact actcgatgac
     1861 ggtaacctga gctacagcgt gcaacaggga tataacagcg aggggaaaac ggctaatggt
     1921 agcgccagca tggactacaa aggggcgttt gcagatgccc gagtgggcta caactacagc
     1981 gataacggca gtcaacaaca actgaactac gctctttcag gcagtttagt tgcccattca
     2041 cagggtatta ccctggggca atcgctgggg gaaactaacg tgctgattgc agcaccaggc
     2101 gcagagaata ctcgtgtggc gaacagcacc gggctgaaaa ctgactggcg cggatatacc
     2161 gttgtcccct atgccacttc ttatcgggaa aatcgaatcg cacttgatgc ggcatcgtta
     2221 aaacgtaacg tggatcttga aaatgcagta gtcaacgtgg ttcccaccaa aggggcgttg
     2281 gttctggcgg agttcaatgc ccatgcggga gcaagggtat taatgaaaac atcaaagcag
     2341 ggtataccgc tgcgttttgg cgcgatagcg acgctggacg gcatacagac taatagcggc
     2401 attattgatg atgatggctc gctctatatg tccggtttgc ccgctcaggg agcgataact
     2461 gtacgctggg gcgaagctcc cgatcaaatt tgtcatatca gttaccagct taccgaacaa
     2521 caaattaact ctgcgattac gcgaatggat gccatatgca gataatcttt ggagaaaaat
     2581 gcgtgttatt actacgacta ttttttgccg ccgtcttaat gctatggtgc gctcaaactg
     2641 ctgcttatat cgggcagtgt cataccactc aggggaatcc gtatattggc gtcaattttg
     2701 gcgttaaaac tctggaggaa gaagaaaata cggctggagt agttaaagac aaattttatc
     2761 agtggaacga atcgaatgat tattatgttt cctgtgattg cgataaagac aatgtcagaa
     2821 gtggccgatg ggcattcgcc gcggattcac cgttagtcta tttaggcgac aactggtaca
     2881 aaattaatga ctatcttgcc gccaaagttt tattgcaggt taaaggcagt tctcctactg
     2941 cggttccttt cgaaaacgtg ggcacagggg cagatacacg atggcatatt tgcgatcccg
     3001 gcggtcaacg tttaggtggc cagggggcta gcggtaatag cggtagcttt tccctgaaaa
     3061 tattgcagcc gttcgttggt tcggtcgtca ttcctcctat ggcgctggcg cgattatttg
     3121 aatgctacaa catacccgca ggtgattcct gcacgactac tggtacaccg gttttagtgt
     3181 attacctgtc tggtacgatc aattcacttg gctcatgttc cgtcaatgcc ggagaaacaa
     3241 tcgaggttga tctgggcgac gtatttgcgg ctaactttcg tgttgtaggg cataagcctc
     3301 ttgaggccag aacggcagaa cttgcaattc cggtcaggtg taacacggga aacgcagggt
     3361 tagttaacgt caatctgagt ctgacggcaa cctcagaccc cagctatccc caggcgatta
     3421 agacgtcacg tcctggcgtg ggcgtggtgg tgaccgatag ccagaacaac attatttccc
     3481 ctgctggtgg gacactaccg ctctctattc ctgatgatgc agacagtatc gcgcgaatga
     3541 atgtctatcc agtcagcacg acaggcgtac caccagaaac cgggcgattt gaagccacgg
     3601 caacggtgag aataaatttt gattaacgcg tgaacgtatg aagaaaaaaa cgatatatca
     3661 gtgcgttatt ttgttcttta gccttcttaa catccatgtc gggatggctg ggcctgaaca
     3721 agtcagtatg catatttatg ggaatgtggt cgatcagggc tgtgatgtcg ccaccaaaag
     3781 tgcattacaa aatattcata ttggtgattt taatatcagt gattttcagg ccgcgaatac
     3841 cgtaagcact gctgctgatt tgaatattga tatcaccggt tgtgccgctg gtattactgg
     3901 cgcggacgtc ctttttagcg gcgaggctga cacccttgcg ccgacactgc tcaaactaac
     3961 tgacacaggc ggaagcggtg gtatggcaac ggggattgcc gtgcaaattc ttgatgcgca
     4021 aagtcagcaa gaaatcccgc tcaatcaggt ccagcctctt acgcccttaa aagccgggga
     4081 taacacactc aaatatcaac ttcgttataa gtccacaaag gcgggagcaa cgggcggtaa
     4141 tgcgacggcg gttctctatt ttgatctggt ttaccagtga aggagtgatg ttgaaacgga
     4201 taatctggat tctgttctta ctgggattaa cggggggctg tgagctattt gcccatgatg
     4261 gcacggtcaa cattagcgga tcgtttcgcc gtaatacatg cgtgctggca caggatagca
     4321 agcaaatcaa tgtgcagtta ggcgatgtct cgctgacacg tttttttcat gggaattatg
     4381 gcccggagaa atctttcatc attaatttgc aggattgcgg aacagatgtc agcacggttg
     4441 acgtcacctt ttcaggaacc cccgacggag tgcagagcga aatgctgtcc attgaaagtg
     4501 gtacggatgc agccagtggg ctggcaattg cgattctgga tgatgcgaag atattgattc
     4561 cgcttaacca ggcgagtaaa gactacagcc tacatagcgg taaggtgcca ttaacttttt
     4621 atgcgcaatt gcgacctgtc aatagcgatg tgcagtcggg taaagtgaat gccagcgcga
     4681 catttgtact tcattatgac taatacgtgg aacagattag cgctcttgat tttcgccgtt
     4741 ttatcgctgc tggtggcggg tgaattgcag gcaggagtgg tggtcggcgg aacgcgattt
     4801 atctttccgg cagacagaga atcgatatct attttactga ctaataccag tcaggaatcc
     4861 tggcttatta acagcaaaat caatcgccca acgcgttggg ccggggggga agcgtcgaca
     4921 gtgccagcac tattactggc cgctccgcca cttattctcc tgaagcccgg tactacaggc
     4981 acgttgcgct tgctgagaac ggaaagcgac atcttgcctg tggatcgcga aacgctattt
     5041 gagttaagca ttgccagcgt gccatccggc aaagttgaaa atcagagcgt aaaagtggcg
     5101 atgcgctcgg tatttaaact gttctggcga cccgaaggtt tgccgggcga cccgctggaa
     5161 gcttatcaac aattacgctg gacacggaat tcgcagggtg tacaactcac taacccaacg
     5221 ccttactaca ttaacctgat tcaggtgagc gtaaatggta aagcgttaag taatgcggga
     5281 gtcgtgccgc ctaaaagcca gcgtcagaca aactggtgtc aggtaatagc accgtgtcat
     5341 gtcgcctggc ggacgataaa tgattacggc gggttgagtg caaagaagga gcaaaatctg
     5401 ccctgaaaca ggttctgaaa acgtttgcgt tttttttgcc gcaggtcaat tcccttttgg
     5461 tccgaactcg cacataatac gcccccggtt tgcacaccgg gaatccagga gagttcatgt
     5521 actacccctt cgttcgtaaa gcccttttcc agctcgatcc agagcgcgct catgagttta
     5581 cttttcagca attacgccgc attaccggaa caccgtttga agcactggtg cggcagaaag
     5641 tgcctgcgaa acctgttaac tgcatgggcc tgacgtttaa aaatccgctt ggtctggcag
     5701 ccggtcttga taaagacggg gagtgcattg atgcgttagg cgcgatggga tttggatcga
     5761 tcgagatcgg taccgtcacg ccacgtccac agcctggtaa tgacaagccg cgtctctttc
     5821 gtctggtaga tgccgaaggt ttgatcaacc gtatgggctt taataatctt ggcgttgata
     5881 acctcgtaga gaacgtaaaa aaggcccatt atgacggcgt cttgggtatt aatatcggca
     5941 aaaataaaga tacgccagtg gagcagggca aagatgacta tctgatttgt atggaaaaaa
     6001 tctatgccta tgcgggatat atcgccatca atatttcatc gccgaatacc ccaggattac
     6061 gcacgctgca atatggtgaa gcgctggatg atctcttaac cgcgattaaa aataagcaaa
     6121 atgatttgca agcgatgcac cataaatatg tgccgatcgc agtgaagatc gcgccggatc
     6181 tttctgaaga cgaattgatc caggttgccg atagtttagt tcgccataat attgatggcg
     6241 ttattgcaac caataccact ctcgatcgtt ctctggttca gggaatgaaa aattgcgatc
     6301 aaaccggtgg cttaagtggt cgtccgcttc agttaaaaag cacagaaatt attcgccgct
     6361 tgtcactaga attaaacggt cgcttaccga tcatcggtgt tggcggcatc gactcggtta
     6421 tcgctgcgcg tgaaaagatt gctgcgggtg cctcactggt gcaaatttat tctggtttta
     6481 tttttaaagg tccgccgctg attaaagaaa tcgttaccca tatctaatta tttcttcgaa
     6541 ttcctcttta taagataatc agggctttat tttcagccct ggttgtttta tattcatcgt
     6601 tgttgcttat ttagacattt tgtactttta ttgttgaggt tattaagcga agcgacaatg
     6661 gattgtgttg ctgcggtttt atagggtagg ggagaggcag atgcgaatta aaccagacga
     6721 taactggcgt tggtattacg atgaagagca cgatcgaatg atgctcgatt tagccaatgg
     6781 tatgctattt cgctcacgtt ttgcgcgcaa aatgttgact ccagatgcct tttctcccgc
     6841 aggcttttgc gttgatgatg ccgcgctcta tttctctttt gaagaaaagt gccgcgattt
     6901 taatttatct aaagaccaaa aagccgaact ggtgctgaat gcactggtag caattcgtta
     6961 tctcaaaccg caaatgccga aaagctggca ttttgtctcc catggtgaaa tgtgggtgcc
     7021 catgccgggc gatgcggcct gcgtttggtt gagcgatact cacgagcagg tcaatttgct
     7081 ggtcgttgaa tctggcgaaa atgccgcgct atgcctgctg gcacaaccct gcgttgttat
     7141 tgcgggtcgc gcgatgcagt tgggcgacgc cattaaaatc atgaacgaca ggctgaaacc
     7201 gcaggtgaat gttgacagct tcagcctcga acaggcagtc taacgcgtca gcttaagggc
     7261 agtcttcggt acacagctac agcaaagaat ggtgccatca tcgcccattg ctgatttttt
     7321 cagcggcgtg acttcgcctt ctaaaagctg aacacggcaa cttccacaaa tgcccgcgcg
     7381 gcaagaataa gggatacgaa ttccctgatt ttctaattgc tccagcaaca cttgttggtt
     7441 atttccacga aatgcctgtc cctgccagtc aatatctaca tttgcgtccg gttgttgcgt
     7501 gatgttggcg gtatcatcag cggcagctgc gccgtaaatt tttgccggag ccgttgccag
     7561 aatttccacc tcatcgccaa cgcgaatcac gccgctatta cgggcaatta aattctgacc
     7621 aaaatcgaca tcgccgttat cctgggcagt gcggaaagat tgcaatgttt ttaatggttc
     7681 gcctgccgga tgtttttgcc ctttttctgg gctgacggtg gtgaaaatac agcggctaca
     7741 aggtttaacc acatcaaaca ccacatcacc aatgcgaatc actttccagc tatcttcttc
     7801 ccaggctgac gcgccggaaa ccaccagatt ggggcggaat tgctccattt ttacactggc
     7861 tggacaacgt tgttggagat cacgtaacga ggcttcgtta gcaagaaggt aaggatagcc
     7921 atcagcaaat gacagaggta cagtgttgtg gcgtttcacg cgccgggtca tttgtggccc
     7981 cacccagcgt aattgcactt cgcgggagaa aaatccactt agccatttgt tgatcgcgtc
     8041 tggcgcaatt cgcgcggtaa aatgtgtgcc ccaaacttcg gttggtgcgt cttgtgtggc
     8101 gaaatcagca aaacgaacat atgcgctact gccatctggt gcggtgagat gcaagccatc
     8161 atgcacaggt gaaggagtaa accgtaccat ctggggaaac tggcgagcgg taataaacgt
     8221 accgtcaggt tccgtgatca taaagatgcg atcgaaggcc agaccactga catcagccag
     8281 agcatgtgta agaccaatgc cgcgcatcga tttaacagga tgaataaaaa gccgggttaa
     8341 tgtcgccacg gtgcggtcct caaatgaaaa taagccctca actttatgac atgacgcgct
     8401 tattagctat aatgcgcaac aattttctta gcgcctgtcc cgttaggcta ttttatttgt
     8461 cagtttggcc ctgggttgtg ctcgaaatcc tcacgtactt gtgtacgctc cggtttctcc
     8521 gcgctctcca tgtccagact gactgcaaca attacgccta ctgcggtagg ctcttagagt
     8581 aaaagtgacg atatgaattc tctgtttgcc agtacggccc gtgggctgga agagctgtta
     8641 aaaactgaac tggaaaatct gggggccgtt gaatgccagg tggttcaggg tggggtccat
     8701 ttcaagggcg acacacggct tgtttaccag agcctgatgt ggagccgctt ggcctcgcgt
     8761 attatgttgc cgctgggcga gtgtaaggtt tacagcgatt tagacctcta tctcggtgtt
     8821 caggcgatca actggacaga gatgtttaat cctggcgcga ccttcgctgt ccacttcagt
     8881 ggtttgaatg acaccatccg caacagtcag tacggcgcga tgaaagtgaa agacgcgatc
     8941 gtcgatgctt tcacgcggaa aaatctgccg cgtccaaatg ttgatcgcga tgcgccggat
     9001 atccgcgtta acgtctggct gcataaagaa accgccagta tcgctcttga tctgagtggt
     9061 gatggtttac atctgcgtgg ctatcgcgat cgtgctggta ttgcgccgat caaagaaacc
     9121 ctggcagccg ctattgtgat gcgatccggc tggcagtcag gaacaccgct gctcgatccg
     9181 atgtgtggtt ccggtacgtt gctgattgaa gcggcgatgc tggcgaccga tcgcgcacca
     9241 ggcttgcacc gtgggcgttg gggctttagc ggctgggcgc agcatgatga agctatctgg
     9301 caggaagtga aagcggaagc gcaaactcgc gcccgtaaag gcctggctga gtatagctct
     9361 catttttacg gttcggacag cgacgcacgg gtgattcaac gtgcgcgcac taacgcccgt
     9421 cttgcgggga ttggtgaact gatcaccttt gaggtgaaag atgtcgcgca actgaccaat
     9481 ccgctgccga aagggccgta cggtacagtg ttgagcaacc cgccatacgg tgaacgtctg
     9541 gacagcgaac cggcgctgat tgcgctgcat agtctgctgg gccgaatcat gaaaaaccag
     9601 ttcggtggct ggaatctctc tttgtttagt gcctcgccgg atctgctaag ctgcctgcag
     9661 ctgcgtgcag acaaacagta caaggcgaaa aacggcccgc tggactgcgt acagaaaaat
     9721 taccacgttg ccgaaagcac accagacagt aaaccggcaa tggtagcgga agattacgcc
     9781 aaccgcctgc gtaagaacct caaaaaattc gagaagtggg cacgccagga agggattgaa
     9841 tgttaccgcc tgtatgacgc cgatctgccg gaatataacg ttgccgttga ccgttatgcc
     9901 gactgggcgg tggtgcagga gtatgcgccg ccaaaaacta ttgatgcgca caaagcgcgt
     9961 cagcgtctgt tcgatattat cgctgcaacc atttcggtac tggggattgc gccaaacaaa
    10021 ctggtgctga aaacccgtga acggcagaag ggcaaaaacc agtaccagaa actgggcgag
    10081 aagggcgagt ttcttgaagt taccgaatat aacgctcact tgtgggtgaa cctgacggat
    10141 tatctcgata ctggtctgtt cctcgatcac cgtatcgccc gccgtatgct cggtcagatg
    10201 agcaaaggta aagatttcct caacctgttt tcttataccg gcagcgccac cgtgcatgcg
    10261 ggattaggcg gtgcacgcag caccaccacc gtggatatgt cgcgtactta tctggaatgg
    10321 gcagaacgca acctgcgtct gaatggcctg accaggcgtg cgcatcgcct gattcaggcc
    10381 gattgcctgg cgtggctgcg tgaggcaaat gaacagttcg atctgatctt tatcgatccg
    10441 ccaaccttct ctaactcaaa acgaatggaa gatgcgtttg atgttcagcg cgatcatctg
    10501 gcgctgatga aagatttgaa acgtctgctg cgtgcaggtg ggacgatcat gttctcgaac
    10561 aacaaacgtg gcttccgtat ggatctcgac ggcctggcaa aactgggact gaaagcacaa
    10621 gaaattacgc aaaaaacgct ctcccaggat ttcgcccgta accgccagat ccacaactgc
    10681 tggctgatta ccgcagcctg aaaggaatag taatgtcatt aatcagtatg catggcgcat
    10741 ggctgtcgtt cagcgacgcg ccgcttctcg ataacgcaga actgcatatc gaagataacg
    10801 aacgtgtttg tctggtgggc cgcaacggcg caggcaaatc gacgttaatg aaaatcctca
    10861 accgtgaaca agggctggat gacggtcgca ttatttatga gcaagatttg attgtagctc
    10921 gtctgcaaca ggatccgccg cgtaacgttg agggtagcgt ttatgatttc gttgccgaag
    10981 gcattgaaga acaagcggaa tatctgaaac gctatcacga tatttcgcgc ctggtgatga
    11041 acgacccgag cgagaaaaat ctcaacgaac tggcgaaggt tcaggaacag ctggatcacc
    11101 acaacctgtg gcagctggaa aaccgcatca acgaagtgct ggcgcaactg gggttagatc
    11161 ctaacgttgc gctgtcgtcg ctttccggcg gctggttgcg taaagcggca ttaggacgcg
    11221 cgctggtgag caatccgcgc gtattgctgc tggacgaacc gactaaccat ctggatatcg
    11281 aaaccatcga ctggctggag gggttcctta aaaccttcaa tgggacgatt attttcatct
    11341 cccacgaccg ttcgtttatc cgcaatatgg cgacgcgcat tgttgatctc gatcgcggca
    11401 agctggtgac ctatccaggg aattacgacc agtacctgca ggaaaaagaa gaagccctgc
    11461 gcgtggaaga actacaaaat gccgagttcg atcgcaaact ggcgcaggaa gaagtgtgga
    11521 ttcgctaggg gatcaaagca cgccgtaccc gtaatgaagg ccgcgtacgc gccctgaaag
    11581 cgatgcgtcg cgaacgtggt gaacgtcgcg aagtgatggg taccgcaaag atgcaggtgg
    11641 aagaggccag ccgctccggt aagatcgttt tcgaaatgga agacgtttgc taccaggtta
    11701 acggtaagca actggtgaaa gatttttctg cccaggttct acgtggcgac aaaattgccc
    11761 tgattggtcc gaatgggtgc ggcaaaacca cgctgctgaa actgatgctc ggtcagcttc
    11821 aagcggacag cgggcgtatt cacgttggca ccaaactgga agtggcttat ttcgatcagc
    11881 accgcgcgga actggatccc gataaaacgg tgatggataa ccttgccgaa ggtaagcaag
    11941 aggtgatggt taatggcaag ccacgccacg tattgggcta tttgcaggac tttctgttcc
    12001 atccgaaacg ggcgatgacg ccagtacgtg cgctttctgg cggtgagcgg aaccgcttgc
    12061 tgctggcgcg tttgttcctc aaaccaagca acttattgat tcttgacgaa ccgaccaacg
    12121 atcttgatgt cgaaacgctg gaactgctgg aagaactgat cgacagctat cagggcacgg
    12181 tattgctggt tagccacgat cgtcagtttg tcgataacac cgttacagaa tgctggatct
    12241 tcgaaggcgg cggtaaaatt ggtcgttatg ttggcggtta tcatgatgcc cgtggtcagc
    12301 aagaacaata tgtggcactc aaacagcctg cggtgaaaaa aagcgaagaa gccgccgcgc
    12361 caaaagcaga aactgtaaaa cgcagcagta gcaaactaag ctataaattg cagcgcgaac
    12421 tggagcagct accgcaattg ctcgaagatc tggaggcgaa gctggaagcc ctacagacgc
    12481 aagtggcgga tgcttccttc ttcagtcagc cgcatgagca gacgcaaaaa gtgcttgctg
    12541 atatggctgc tgcagagcag gagctggagc aagcctttga acgctgggag tatcttgaag
    12601 cgttaaaaaa tggtggctga tcgcaaaata gtcgatttaa acagagacag ctgacaacgg
    12661 taaaattgcc tgatgaactg ggtttatcag gcatgaaaac caacactttg ttagtcatct
    12721 gtaaaatagc gcatcattaa ggagtaccaa tgtgcgaaca tcatcatgcc gcgaagcaca
    12781 tcctgtgctc gcagtgtgac atgctggtgg cgttaccgcg ccttgagcat ggtcagaaag
    12841 cggcatgtcc ccggtgtggc acaacgttaa ccgtggcgtg ggatgcccct cggcagcgtc
    12901 ccaccgccta tgcgttggct gcactgttca tgctgttgct gtccaacttg tttccttttg
    12961 tgaatatgaa cgttgcagga gttaccagtg aaattacatt actggaaatt cccggcgtgc
    13021 ttttttctga ggactacgcc agcctcggca cctttttcct gttgtttgtg caactggttc
    13081 ccgcgttttg tctgatgacc attctgttac tggtgaatcg cgcggaatta ccggtccgtt
    13141 taaaagagca actggcacgg gtgctttttc aactcaaaac ctggggaatg gcggagattt
    13201 tcctcgcggg tgtgctggtc agtttcgtta aactgatggc ttacggcagc attgggatag
    13261 gcagcagctt tctcccctgg tgtttatttt gtgtcctgca actgcgcgct tttcagtgcg
    13321 ttgatcgtcg ctggttatgg gacgacatcg ccccgatgcc agaactgcgc cagccgctaa
    13381 aaccaggcgt cacggggata cgtcaggggc tgcgttcgtg ctcctgttgt acggcaatcc
    13441 ttcctgctga tgaacccgtg tgcccgcgtt gtagtaccaa agggtacgtt cggcgtagaa
    13501 acagcctgca gtggacactc gcgctgcttg taacgtccat catgctgtat cttccggcta
    13561 atattttgcc catcatggtg acggatttat taggctcgaa gatgccgtcg acgattctcg
    13621 ctggggtcat tctgttatgg agcgaaggct cttatcccgt cgctgcggtg atctttctgg
    13681 ccagtattat ggtgccaacg ttaaagatga tcgccatcgc gtggctgtgt tgggatgcca
    13741 aagggcatgg caagcgcgac agtgaaagaa tgcatttgat ttatgaagtt gttgagtttg
    13801 taggccgctg gtcgatgatt gacgttttcg ttatcgcggt gctctcggcg ctggtgcgta
    13861 tgggaggttt aatgagtatt tatccggcaa tgggtgcatt aatgtttgct ttagtcgtca
    13921 taatgacaat gttttctgct atgacgtttg acccgcgttt gtcgtgggat cgtcaacctg
    13981 aatcagagca tgaggagtcc tgacagtatg gaatctaata atggggaagc caaaatccag
    14041 aaagtgaaga actggtctcc cgtgtggata tttcctatcg tcacggcgct cattggggcc
    14101 tgggttcttt tttatcatta cagccatcag ggaccggaag tgaccctgat caccgcgaat
    14161 gcggaaggaa ttgaaggtgg caaaaccacc attaaaagcc gtagcgttga cgtcggcgtg
    14221 gttgaaagcg ccacactggc tgatgatttg acgcacgttg aaatcaaagc gcggctgaat
    14281 tccggtatgg aaaaattgct gcataaagac accgtctttt gggtggtgaa accgcagatt
    14341 ggtcgcgaag ggattagcgg cctgggaacg ctgctgtctg gagtttatat cgaactgcag
    14401 ccaggcgcga aaggcagcaa aatggataaa tacgatttgc tggactcacc accgttggcc
    14461 ccgcctgatg cgaaaggtat ccgtgtgatt ctcgatagca aaaaagccgg gcagctctcg
    14521 ccaggagatc cggtgctgtt ccgtggctat cgggtaggtt cggttgaaac cagcaccttc
    14581 gatacgcaaa aacgcaatat cagttatcaa ctgttcatca atgcacctta tgaccgactg
    14641 gtgaccagca atgttcgctt ctggaaagat agtggcattg cggttgatct gacgtcagcg
    14701 gggatgcgtg tggagatggg ctcattgaca acgctgctta gtggcggtgt tagctttgat
    14761 gtgccggaag gtctggattt agggcagcca gtggcaccga aaacagcttt cgttttgtat
    14821 gatgatcaga agagcattca ggattcgttg tacaccgatc acattgatta tctgatgttc
    14881 tttaaagatt cggtacgcgg tctgcaaccg ggagctccgg tagaattccg gggtattcgc
    14941 ctgggtaccg taagcaaagt gccattcttt gcgccgaata tgcgtcagac atttaacgat
    15001 gattaccgta ttccggtact gattcgtatc gagccagagc ggctgaaaat gcagcttggc
    15061 gaaaatgcgg atgttgttga gcaccttggc gaattgttga aacgtggttt acgcggatcg
    15121 ctgaaaaccg gaaacctggt cactggtgca ctgtatgttg atctcgattt ctatccaaat
    15181 acgcctgcaa taaccggtat tcgtgaattt aatggttatc agattatccc gactgttagc
    15241 ggcggcctgg cgcaaatcca gcaacgactg atggaagcgt tggataagat caacaaactg
    15301 ccattgaatc cgatgattga acaggcaacc agtacgcttt ctgaaagtca gcgcacaatg
    15361 aaaaacctgc aaacgacgct ggatagcatg aacaagatcc tcgccagcca gtcgatgcag
    15421 cagttgccga cggatatgca gtcaacgttg cgtgaattga atcgcagcat gcagggcttc
    15481 cagcctggct ccgcagccta caacaagatg gtggcggata tgcagcgcct tgatcaggtg
    15541 ttgcgagaac tgcaaccggt gctgaaaacg ctcaatgaga agagtaacgc gctggtattt
    15601 gaagcgaagg acaaaaaaga tccagagccg aagagggcga aacaatgaaa aagtggctag
    15661 tgacgattgc agcactgtgg ctggccggat gcagctccgg cgaaattaat aaaaactatt
    15721 accagttacc tgtggtgcag agcggtacac aaagtaccgc cagccagggc aatcgtctgt
    15781 tatgggtaga gcaggtcact gttcctgact atctggcggg gaatggtgtg gtttatcaaa
    15841 ccagtgatgt gaagtatgtg attgccaaca acaacttgtg ggccagcccg ttggatcaac
    15901 agttgcgcaa caccctggtt gccaacctga gtacgcaact gcccggctgg gtggttgcct
    15961 cccagcctct gggaagcgcc caggacacgc tcaatgttac cgtaacggag tttaacggtc
    16021 gctatgatgg caaggtcatt gtcagtggtg agtggctgtt gaaccaccag ggacaactga
    16081 tcaaacgtcc gttccgtctg gaaggagtgc aaactcagga tggttacgat gagatggtta
    16141 aagtgctggc cggtgtctgg agtcaggaag ccgcttctat tgcacaagag ataaagcgtc
    16201 taccttaatt ataaagattt gtaaatataa ccgtctccgg tatgttgcct gaggcggttt
    16261 ttttgtttct aacgtgcgga aaaatttgtt cctcttcaca ttttttgtac aaccgacatg
    16321 cccgtgtagc tcacaaatat gacagtggcg tgaattttgc gcattgacgg cagttatgat
    16381 tcgcggtatt gcttaactgt gattgcacat ttagtaatca ctgttttctt ttccaccaga
    16441 aaccagtatg agggaaacga ggcatgaaga gacaaaaacg agatcgcctg gaacgggcac
    16501 atcaacgtgg ttatcaggcc ggcatcgccg gacgctcaaa agaaatgtgt ccctatcaga
    16561 cgctgaatca aaggtcacaa tggctgggag gctggcgaga agccatggcg gacagggtag
    16621 taatggcctg attctgtctc tttaaaaaga aacctccgca ttgcggaggt ttcgcctttt
    16681 gatactctgt ctgatgataa tcagaaggca gacgtatcct ggaacagacc gactttcagg
    16741 tcgctggcgg tatagatcag acgacaatca accagcactt cgccatctgc cagacccata
    16801 atcagacgac gattaacaat gcgtttaaag tgaatacggt aggtcacttt tttcgctgtc
    16861 ggcagtacct gaccagtgaa tttcacttcg ccaacgccca acgcgcggcc tttaccttcg
    16921 ccgcccagcc agccgaggta gaaccctacc agctgccaca ttgcgtccag gcccaggcat
    16981 cccggcataa ccggatcgcc aatgaagtgg catccgaaga accacagatc cggattgata
    17041 tccagttctg cttcaacata ccctttgtcg aagttaccac ccgtttcggt cattttgacc
    17101 acacggtcca tcatcagcat gttcggtgct ggcaattgcg ggcctttagc gccaaacagt
    17161 tcaccgtgac cagaggcaag aaggtcttct tttgtatagg attcgcgttt atctaccatg
    17221 ttctctgtaa gccttatttt attgaagcac gcaggatagc taacacgtgt acgctgaaca
    17281 agtccgatca gttcggaata aaccagttca gccaacgtaa tggccatgga aaacggtgac
    17341 gtccttcctg ttgcgatgct tgcgcgatac gttcctggat ggtttgcatc agcgtcgttt
    17401 ggccttcgcc atcccacacc agatttaata ataacggcag tgcgtcagtc acatcgtcta
    17461 ctgcccagat ggtgaatttg ccttcttcta ccgctttcac cagttcactg tgaagactta
    17521 aatggcgaac gttagcagtg gggataatga caccttgttt ccccgttaac tcacgttgct
    17581 ggcaaatagc aaagaagcct tcgattttct catttaaacc accgaccggc tggacgcggc
    17641 cgaactgatc gactgaacct gtgatagcga tactctgatt cactggcaca tcagcgaggg
    17701 cgcttatcag ggcgcagagt tcagccatcg aggcactatc gccatccact tcactgtatg
    17761 actgctcaaa tgtcagcgat gctgagaagg ggatctgttg ctcaagctgt agttccgaca
    17821 tcaggaacgc ctgcatgatc atcatccctt tcgcatggat attgccgcca agctccgctt
    17881 tgcgttcgat gtcggtgaat tcaccatcgc caatatgcac aacgcagcta atgcgagaag
    17941 gttcgccaaa agcgcgtgga tgacccggaa attcaatgac cgaaagggcg ttaatttgcc
    18001 cgatgcgttc gccttcggtt tcaatcagga tttgctcctg aaggatctca tcctgcatac
    18061 gttcagcgag gaaaccttcg cgccattcac gctgctgcag cattaagttt agctgctcgc
    18121 cggagaaggt gtcgccatca cacagggagg cgacctcttt acactggcgg aggatccact
    18181 gcgggctaag cggaagtgtt tcttgttcac cggtgtagcg tgctgcttcg cggataagta
    18241 ccggccaagc atccgctccc ggggcaggta agtgattatg tctggcggta aatgtcaccc
    18301 agcgacacca ctggcttact gactccgcat cgacaatctg cagagtatct tcaaattcgc
    18361 tataaatagc ctgctctgaa agctctggct ccatctcctg gaaatcagcc aatgattcgc
    18421 gttcgcctac cagaatgact ttcagcttca atggcatcga aggcacagag acggggagag
    18481 ggcgcgactc atcaaacgca acccagtcaa aacgctcgcg gttaacgata tttttcagcc
    18541 gcatccacag cagaggttgc gccagcagtg tacgcaaaga gatgatgaga ataccgccat
    18601 ttgcctgatg caccagacca ggctgcaggg taatgtcgcc attaaactgg cgcaggcagc
    18661 caaagagttg ctccgcttct acccagtcgg cagcgacaac ttgcgttaaa gtcgcaaaat
    18721 tatcatctgc actcactgcg tgacgtaagc ggatggagtg gccggaaacc tcatagtgat
    18781 cgcccaccag ttggcctgca tcgctttgta gcgtacgcgc ggcatcggca ataagattca
    18841 gatactcaga ctcttccggg gccttagcca gcataaaaga ggaggatgct cgcgtatgca
    18901 gcaactgctc cagcgcaaat tgcagtcgcg gttgagtatc actgaataaa ggatcgtttt
    18961 cgtcaatcaa atgtggctga gcaaatattt cctgatagct atcggtatca ggaaccaggt
    19021 cacgccatgc aagtttcgta atggtcaaag ttgatgtttt ttagtctgtt gtcaaagccg
    19081 cgattatacc gtaaccggca ctacagcaca cgtagaaagc accgacaata ctcttggctg
    19141 ggcgttaaag ctcacaggat ggagattctt ttcttcactg gcctaaaaag ctgatattct
    19201 gtaaagagtt acacggtaac attgagatcg ctatgaaata tcaacaactt gaaaatcttg
    19261 aaagcggttg gaaatggaag tatctggtga agaagcatcg cgaaggggag ttaattaccc
    19321 gttacataga agccagtgcc gcccaggaag ccgtcgatgt gttgctctcg ctcgaaaatg
    19381 aacccgtgct ggtaaatggc tggattgaca agcatatgaa tccggaactg gtcaatcgca
    19441 tgaagcagac cattcgggcc agacggaagc gccattttaa tgcggaacac cagcatacgc
    19501 gcaaaaaatc gatcgatctg gaatttattg tctggcaacg tctggctggt cttgcacagc
    19561 gtcgcggtaa aacgctttct gaaacgattg ttcagctgat tgaagatgcg gaaaacaaag
    19621 agaaatacgc gaataaaatg tcttctctga agcaggatct gcaggcattg ctgggtaagg
    19681 aataactgac gaaagtcagt tcaatttact aaaggcaaaa aaaaccccgc agcagcgggg
    19741 tttttctacc agacgataac ttaagcctgc ggctgagtta caacgtcttt gataccttta
    19801 acttcgatct ctacgcgacg atccggagcc aggcagtcga tcagtgcagc acgctgtttc
    19861 acgttgtcac aggtgttgcc agtaaccggg ttggattcgc ccataccacg tgcggagatc
    19921 ttgtctgccg ggataccttt ggagatcagg taatcaacaa cagactgagc acggcgctcg
    19981 gacagaccct ggttgtaagc gtcagaaccg atgcggtcag tgtaacccag aacaactacg
    20041 gaaccgtctt tcggatccag gttgctcagc tggctgtaca gctgatccag agcagcctga
    20101 ccttccggtt tcagggttgc tttgttgaag ttgaacagaa cgtcagactt cagagtgaag
    20161 tgcttggtct gtacttccgg tgccggagct ggagcaacta ccggagctgc ttcgccctga
    20221 ccgaaacggt aggaaacacc caggctcagc agaccgttgt ccggacgagt accgatggtg
    20281 tttgcgtcac cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg
    20341 atcgcgtact caacaccacc tgcgaaaacc ggagaaacgc cagtgtcgtg gtctttaaag
    20401 gatgcgccac taggtacgtt agccttggtg tctgcacgcc ataccatacc acccagacga
    20461 gtgtagatgt ccagatcgtc agtgattggg taacccagtt tagcggtcag ctgaacgccc
    20521 tgagctttgt atgcgccgtt gatgttgtcg cctttgtacg gcatacgacc taaccagtcg
    20581 taacccattt caaaaccaac atacgggtta acctggtaac caccaaaagc acctgcaccc
    20641 agttggtttt cgtgggtcgg accattgtta ggaataaaac cagtgtcatg gtactgggac
    20701 cagcccagtt tagcaccagt gtaccaggtg ttatctttcg gagcggcctg cgctacggta
    20761 gcgaaaccag ccagtgccac tgcaatcgcg atagctgtct ttttcatttt ttgcgcctcg
    20821 ttatcatcca aaatacgcca tgaatatctc caacgagata acacggttaa atccttcacc
    20881 gggggatctg ctcaatatta actctaccga tatcttcggc ttatgccgag cacccctagc
    20941 gatgtaaagt ctacaacgta gttgaaaact tacaagtgtg aactccgtca ggcatatgaa
    21001 aaaaaagtct tgtataaggt atgtttaatc tttttgtcag cgacaattta cagaaaaaaa
    21061 acgcggaaac cgcttcagac aagccttcgc aaggaaaatt agtcacgact gaaagcattg
    21121 gctgggcgac aaaaaaagtt ccaggattaa tcctaaattt acttaatgat acaaattaga
    21181 gtgaattttt agcccggaaa gttgtctcgt tgtgtgagag gatgcgctta ccggacgcat
    21241 aataaacccc atagcgttac cttcatttgc cgcatcaaca agttcagcat gctcttcttc
    21301 agtcaaatca tctgtcaacc aaccgatcac cacactgtaa ttgcccgtgc gtaaagcgcg
    21361 aaccattgac tccacagtgt ggcaagggga gagctggcta atctgcatta ctttcgttaa
    21421 gggtagccca gatgcctgaa cccattcccg actcagtttt tgttgcggtg ttaaccagag
    21481 ttgccagcgc gattgctgac cgagttgctg taacaatggc aacagtagaa gttgcgtcat
    21541 catgggctga tcttcgcgat agacaacttc actgataagc ccggctgtag tgttttccgt
    21601 agagacacgc gcaattttac ttgctgcgga tgagaacgac gaagaacgat gtgcatagcc
    21661 tgaagtgtac ataatcaatc cagcccctgt gagttactgt atggatgtgc agtacatcca
    21721 gtgacaacaa agatcaaccc tattttcgga aagagcctcg caaattttgt cgttggtgac
    21781 gggaaaacat aaattaatct tgccccttaa gaataagttg cctattttcg tagttaacgg
    21841 atccgttaat gtgaatcatt cttttatgtt atgattttaa aaggaatttt atgaaaagcc
    21901 tctcctataa gcggatctat aaatcacaag aatacctggc aacgttgggc acaattgaat
    21961 accgatcatt gtttggcagt tacagcctga ccgttgacga cacggtgttt gcgatggttt
    22021 ctgatggtga gttgtatctt cgggcttgtg agcaaagtgc acagtactgt gtaaaacatc
    22081 cgcctgtctg gctgacatat aaaaagtgtg gccgatccgt taccctcaat tactatcggg
    22141 ttgatgaaag tctatggcga aatcaactga agctggtgcg tctgtcgaaa tattctctcg
    22201 atgcagcgct gaaagagaaa agcacgcgca atacccggga aagactgaaa gatttgccca
    22261 atatgtcttt tcatctggaa gcgattctcg gggaggtggg gattaaggat gtacgggcgt
    22321 tacgtatact tggggcaaaa atgtgttggt tgcgactgcg gcagcaaaac agtctggtga
    22381 cagaaaagat tctgtttatg cttgaaggtg ccattatcgg cattcatgaa gctgcgctcc
    22441 cggtggcacg ccgccaggag cttgcagaat gggctgactc tcttacgccg aaacaggagt
    22501 ttcctgcgga gcttgagtaa tctggcgttg cagacgacca atctctggca gtaatgcaat
    22561 caataaccca acttgttgta cgaccagagg ttctttgcta tctgcccgtg gttcaagttg
    22621 ctgcatccgc tgtttcaggc cagctaatgc ctgattgacg cgttcttcat cagcaggttg
    22681 atgatgtaac gcgtcatcaa catagcaaac tgcgtcatca agaaacgcca gaatttcagg
    22741 attagttaac tgctcccggt gagcaccgag ggctgagata tagctggtaa acgtatggtt
    22801 aaggcacagc aagcgaaacg cggcttcgcg aatttgcggg gtaacgttcg gttcgctgga
    22861 catatttgat accaccgacg ccagttcagc atcacggttg tgtgcatcgc ggcgggcaat
    22921 acgatacgcc agacggttat cacgcccctg atggtattgc tccagtatgg catcgagata
    22981 gcgacagttg gcctctgtgg cgcgttcgag cattcgcggc agattgcgaa acttccagtc
    23041 aggccagatg tagctcactg ccgcccacgc aatggcacaa ccaatcagcg tatcgattac
    23101 gcgaggtaac gctacttcaa aaccttcacc cagtaagtta aaacacagta gcaccaaaag
    23161 tgtgatgaac atcgttgcat gagcgtattg cacgttacgg aaggcaaaaa agagcacgcc
    23221 ggtaataacc agcagcacca gctgcccttc cagtgatggc acaaaccaca gcacaggaat
    23281 gccaatcgcg atacctacca gcgtaccaat aatccttaac ttcaggcggt ggcgcgtggc
    23341 gttatagttt ggctggcaga cgaacaaact tgtcagcaag atccaatacc cgtgatgcat
    23401 tccggttatt tgaatgatgg cgtagccgaa gcacaacacc agcgacattc ttaccgcatg
    23461 acggaagagg gcggattccg gcgtgaagtg acggctaaga cgcagccaga tatcactcaa
    23521 tccgtgcggg ctgtcatcag cgagctcatt ttcgtcatta ttacggggta gtgcctgggc
    23581 ctgttctgat tcaattgttg ccagttgggc atcaatggca cgtaaattgt tcagcaaaaa
    23641 tcccagtgtt ttgagtaaat cggcgggtgc gccgttatcg cgcatccgct ccagcgcagc
    23701 atcaatatgc gtaaaagcgc gctcaaaatg cggatcatgt tgataagact gacgcaacaa
    23761 aatacagcgt gacagttgct ggcacgcctg gccctgcatc gacatcagcc gctgaaaacg
    23821 gaacagcacg tcgctgtggc gaaaatgttc acgcaatgtt tgatactgaa tatgagaaga
    23881 gctggcacgc tcgtgaatat cctgtgcgac aaagtaataa tgcagcgtgc gacgcgttcc
    23941 ccgttgacca cgatcgccac gtaagcgggt cagcagcgag agtttcgtct gattcaatgt
    24001 cgccatcagc tgaccgttgg cgagagccaa atcgtacagc ggtgcctggc tttcatcttc
    24061 aatatcagga tcaaacatgc gcgacttgag ctcaagataa cgcgccagtt gttcatagca
    24121 acgcgccagg ttgtcctgca gcgggcggac cgggaacagc agatgaccaa taagtgtcag
    24181 gacgttgtac cagacggcac cggccagcag atacatcggc tgctgatacc agtgctcata
    24241 cagtgatgtt cccaacatag tgtaaatggc gatcagcaat gcaccgaagg caattgttgc
    24301 atagcgttga cccagaccgc cgagcaaaat gaagccgctg gtagagagcg ttaagccaat
    24361 cgcaaatagc cagggccagg gaaacagcaa ttctactgag gccgaggcga taaaaaagca
    24421 gaacagcgta atgatgaggt tacgcaaacg tcccgccagt cggtcatcga gatcggtcag
    24481 cgccgctgcc accatcccca gcgttagcgg aatcgtcagt tttacatcac ccagccacca
    24541 cggaaacgct gtggttccac aaagcgcaat aaaaatacgc gcgtaataca gccaggcgct
    24601 gttccaggta tagcgtttga gcaaaggact tagcataaag gccatattag tggattattc
    24661 aaaacgacga cgtgcattgg cttcacgcgc agcttgcgct gtttctaccg ataccacgcg
    24721 acgaccaacc ggccatagtg caatagcggc aattttaaag ttcgcaatgc cgacaggaat
    24781 gccaatgatt gaaatacatt gtgcgatgcc cgttgcaatg tgcatcaggc ataaccacca
    24841 gccaaagaaa atcagccaga aaatattcaa taccgtaccg ccagtattca gcaacacatt
    24901 tttgccagcc gggttcagtt catcgacatg aatagcttca ttgccataag gcaccagaga
    24961 cagtttagtg atctcccagc aggatcgtgt cagcggtaag gtaaaaatca gcacaatact
    25021 gaccagagtc gccaacagcc agcccagagt ggtggcaaat ccgccaagca caaagttcag
    25081 aatgttcaaa acggtacgca taaaacctcg ctttactgtg gttttcagta atgggcggca
    25141 attgtaacgt ttttttgggc tggagcacgt tttctctgac ggttacactg ataagaaata
    25201 atttcgtgtg gatctacaga gtcatggaac tgaaagcgac aacgcttgga aaacgtctgg
    25261 cacagcaccc ttacgatcgg gcggtgatcc tcaatgccgg gattaaagtc tccggcgatc
    25321 gccacgaata ccttattcct ttcaatcaat tactggcgat tcactgtaag cgcggtctgg
    25381 tatggggcga gctggaattt gtactgccgg acgaaaaagt ggtgcgtctg cacggcacag
    25441 aatggggcga gacgcagcgt ttttaccatc atcttgatgc tcactggcgg cggtggagtg
    25501 gcgagatgag cgaaattgcg tctggtgttt tacgccagca actggatttg attgccacgc
    25561 gcactggaga aaataaatgg ctgacgcgtg agcaaacctc tggcgttcag caacaaatcc
    25621 gccaggcttt gtcggcgttg ccgttgccgg ttaaccgact ggaagaattc gataactgcc
    25681 gtgaggcgtg gcgtaaatgt caggcttggt tgaaagatat tgaaagcgct cggttgcagc
    25741 ataaccaggc gtataccgaa gccatgctta ccgagtatgc ggattttttc cgccaggtcg
    25801 agtcttcacc gctgaatccg gcgcaggccc gggcagtcgt taatggcgag cattctctgt
    25861 tagtgctggc aggtgcagga agcggaaaaa cgtcggtgct ggtggcccgt gcaggctggt
    25921 tgctggcgcg tggtgaagcg tcccctgagc aaattttatt gctggcgttt ggtcgcaaag
    25981 ccgctgaaga gatggacgag cggattcgcg aacggctaca taccgaagac attaccgcac
    26041 gcacgtttca tgcgctggcg ctgcatatta ttcagcaggg cagcaaaaaa gttccgatag
    26101 tcagcaaact ggaaaatgat accgctgccc gtcatgaact ctttattgct gagtggcgca
    26161 agcaatgcag cgaaaagaaa gcgcaggcga agggctggcg gcaatggctg acggaagaaa
    26221 tgcagtggtc agtgccagaa ggtaacttct gggatgatga aaaattacag cgtcgccttg
    26281 cctctcgcct cgatcgctgg gtaagtctga tgcggatgca cggtggtgca caggcagaaa
    26341 tgattgccag tgcacccgaa gagattcgcg atctgttcag taaacgtatc aagttgatgg
    26401 ccccgttatt aaaagcctgg aaaggtgcgc tgaaggcaga aaacgctgtc gatttttcgg
    26461 gccttattca tcaggcgatt gtgattctgg agaaaggccg ctttatcagc ccgtggaagc
    26521 atattctggt tgatgaattt caggatatct cgccgcagcg cgcagcgttg ttagcggcat
    26581 tacgcaagca aaacagtcag acgacgttgt tcgccgttgg tgatgactgg caggcgattt
    26641 accgattcag cggtgcgcaa atgtcgctca ccaccgcttt ccatgaaaac tttggtgaag
    26701 gcgatcgctg tgatttagac acgacttacc gttttaacag tcgtatcggt gaggtggcaa
    26761 accggtttat tcagcagaac ccaggccagc tgaaaaagcc gctaaacagc ttaaccaatg
    26821 gagacaaaaa agccgtcacg ttattggatg agagtcaact tgacgctttg ctggataagc
    26881 tctctggtta tgccaaaccg gaagagcgca ttctgatcct ggcgcgttac catcacatga
    26941 ggcctgccag cctggaaaaa gcggcaacac gctggccgaa gttgcaaatc gactttatga
    27001 ccattcatgc cagcaaaggg caacaggcgg attacgtcat catcgttggc ttgcaggagg
    27061 ggagtgatgg ttttccggct gcggcgcggg agtcgattat ggaagaggcg ctactgccac
    27121 cggttgagga tttcccggac gctgaagaac ggcgtttaat gtacgtggcg ctgacccggg
    27181 cacgccatcg ggtatgggca ctgtttaaca aagagaatcc ctctcccttt gtggaaatac
    27241 tgaaaaatct ggatgtgccg gtggcgagaa aaccgtaaga aacaggtggc gtttgccacc
    27301 tgtgcaatat tacttcagac ggtcagcgag ataacgctga taatcgggga taagaatatc
    27361 gaccgcgtcg ttgaaatgcg gcgactggat aatgaagtct gccgttgcca cgttggtggc
    27421 gaccggaatg ttccataccg tcgccagacg cagcaaggct ttcacgtcag gatcgtgcgg
    27481 cacggcattt agtggatccc agaagaaaat caatacatca attttccctt ctgagatcaa
    27541 tgcgccaacc tgctggtcac cccccattgg gccactcaac atcgcgttga cgttcatgcc
    27601 ggtcgcgcgg gaaatcaagt taccggtagt gcctgttgca tacagtacgt gttgttccag
    27661 taacggttga tgccgttcca cccagctcat cagcatttgt ttgcagtgat cgtgtgccac
    27721 cagcgcaata tgtttccgcg caggtaaagt gcgagtcgtc agttccataa tgtacatccg
    27781 tagttaactt tcctacagat tactgtaagc acttatcgct gcaagataaa gaccgaaaaa
    27841 gcctgcgcac aggcacaaaa atctcaggaa gatggttgtt tttccgccca ttgcaggaaa
    27901 gtatttcgcg tttgtgggtc agccagttta aaccaatact tcagccgttg ttctgtgagc
    27961 acctgagact gcggtggaat agcatccagc tcggaaacac ccgaaagcag cgtactgtca
    28021 tctgccatca tcgtggcaaa ttgcggtagt gaagcgcgtt tcgcggattt gttatagcgt
    28081 tcaacctcga cctcataatc aatcgttttt gccgttgagg taatggcgag aatatccagc
    28141 tttaccggaa tcggcgtcgc atcgccatcc aacaattcaa ggcgcggcgc ggcatcaaaa
    28201 tggttagctt cccgctcatt ctccaggcga ggcaggcgaa aattgacctg gttgatgagc
    28261 tgggtattaa aactgaccac cagcggtggg gagatataca gccgttcttc actattggaa
    28321 agatgaatcg tcttctcaac gcgaaacact aactgatgcg gtccattgtc cagttcaatg
    28381 ctgtccgcac cgcgcagcag agagctggaa accttcttgc cgtcgagtac cagaagatcg
    28441 acatcggttg aaagccgcag cgtggtggca aaaacggata ccggcaaaca taatgcaatc
    28501 aaggtggtca cgatgccggt tttcataggt agctcctgtc aaaagaccgc ccgaataata
    28561 atgtgtcaaa atttttcttt aatcatactt actaacatat agacatattt cccgcttttg
    28621 ctctcattca ttcgtattag ctgcatggtt ggcatgtcgg ctttgtcgta tactttgtta
    28681 aacagtctgg aggaaataat gaaagaaacc gatattgcag gcattttaac gtccactcac
    28741 accattgcgc tggtgggggc aagcgacaaa cccgatcgcc ccagctatcg cgtgatgaaa
    28801 tatctactcg accaggggta tcacgtcatt ccggtttcgc caaaagttgc cggcaaaacg
    28861 ctgctgggcc agcaggggta tggcacgctg gcagacgttc cggaaaaggt cgatatggtc
    28921 gatgttttcc gtaattcaga agcggcgtgg ggcgtggcac aggaagctat tgccattggc
    28981 gcgaaaaccc tctggatgca actgggggtg attaatgagc aagcggcagt actggcacgg
    29041 gatgccgggt taaatgtggt gatggatcgc tgcccggcta ttgagatccc tcgcctgggt
    29101 ctggccaaat aaaaaatccc cggaaggcaa aaaccttccg gggatttgtt cagggattag
    29161 ttacgcagac gcggggcctg gagttgtttg cggatggtct gcgccagctc atccatggat
    29221 ggctgttcag gatgttcatc ctgcaattcg ctacttagct gggcttcagc caggtaggta
    29281 tgaaccggta ggccgttatc gtcctccatc accacgtgat accacggagc ggcgcgaagc
    29341 tcgtcattca ccgccagctc atcaggcgac ggttcagaga gcgaataaac cgggtcgata
    29401 tccacgacca ctccgagata acctaacagg gaatggcgga cctgctggcc gataccgaat
    29461 ttgctggcaa tcatagtcac ctcccgggaa atctttacct ttgatataag ggtaaaattc
    29521 cacatttcaa gttacatgac gcgacaggca aaccctttca gatatagccc ttccgggtag
    29581 gtagcgatca ccggatgatc ggctgcctga cggaactgct ctataaattg tacatcacga
    29641 ccggcatcaa ttgcggcatc cgcgatgatt ttctgaaata aatcgctggt catcagaccg
    29701 gaacaggaga aagtcaggag aataccgcct tcattcagca actgaatcgc cagcatgttg
    29761 atgtctttat aaccacgaca cgcgcccatc aactggcttt tattctcaac aaacttcggc
    29821 gggtccatca cgataacgtc aaatttttca ccgcgatcgc gataagtacg cagcaattta
    29881 aagacatcat cacggacaaa ctcagccttg ctcagatcca gtttgttcag ctcaacgttc
    29941 tgccgtgcaa tatccagcgc ttcctgggag gtatcaacgc tgacaacctg gctgcaaccg
    30001 cccatcagtg ccgatacggc gaaaccaccg gtataggaga aacagttcag cacacgttta
    30061 ttttcaacgt agcggcgggt agccaggcgg ctgtctcgct ggtccagata gtagcccgtt
    30121 ttgtgtccgt gctgaatatc caccagcagc ttcatgccgt gttcttcaat cggcagcagg
    30181 gcaggcggca actcgccggt gacgaggccc tgggtcagct ccatcccttc ttttttacgt
    30241 accgcaacat cgctgcgatc gtaaatcgca cattccgggt acagcgtttg cagggcacta
    30301 attaatgccg cgcgctgata ttctgcgcca gcactcagca gttgcagcac cagaaaatta
    30361 ccgaaacgat cgatagtaat acccggcagg ccatcagatt ctccggcgat taaacgatag
    30421 ctgtcgaggc catctttttg cgccagccag tcacgccatt tttgtgcttg ttgcaaacgg
    30481 cgggaaaaaa aagcaatgtc gatagactca gacgggtcaa acgtccagac gcgcgcccgg
    30541 atttgcgaag ctggcgaata agcgccgcgt gctaaccatt ttccctgatg atcaacaata
    30601 tcgatggttt caccgaggct ggctttacct tccatgcggg caacggcccc ggaaaagacc
    30661 cacggatggc gacgaagtaa tgatttttcg cgccctttgg ctaacactaa acgtacactc
    30721 ataatttact tttctgtcga tgcaaagaaa tgggcgtcat tgtccggagt tttccatgga
    30781 tttgcaacgc gtccattaag gataaggaga gcagcaatgt cgaaagtctg cataattgcc
    30841 tggatttacg ggcgagttca gggcgtagga tttcgctaca ccacacagta cgaagcgaaa
    30901 agactgggct taaccgggta cgccaaaaat cttgacgatg gcagcgtgga agtggttgcc
    30961 tgcggtgaag aagggcaggt agaaaaatta atgcagtggt taaagagtgg cggcccgcgt
    31021 tctgcgcgtg tagaacgggt gctcagcgag ccgcatcatc cctcggggga attaaccgat
    31081 tttcgtatcc gttaaataca ttttaccggt tttggcaggc cggcaatttt ggtggcttgc
    31141 ttagccgggc ctttcgggaa cagtcggtat aaatagcggc tgttaccttt ttcttcacca
    31201 aatttattcg ccatcgcttt taccagcata cgaatcgccg gagacgtatt aaattccagg
    31261 tagaagtcac gtacaaaacg caccacttcc cagtgttctg gcgacagcga aatcccttcg
    31321 ttctctgcaa tcaccactgc cagcggttca ctccactggc tgctttcttt gagatagcct
    31381 tcggtatccg tttctatctc tttaccttcg aagatcagca taattattac taccttaatc
    31441 agactggcgg cagtgtaaca aaaaacaaag ccccgcataa agcgaggcta tgaaagtgtt
    31501 agcgggtgag attaatcgcg gctggcgaag cccagaatgc tcagcaggct gacgaagatg
    31561 ttgtacagcg aaacatacag gctaaccgtg gcacgaatat agttcgtttc accgccatga
    31621 atgatgttgc tggtttcaaa caagatagcg ccagaggaga tcagaatgaa gaccgcgctg
    31681 atcgccagat gcagagcagg cagttgcagg aagatattag caaccatacc aatcagtacc
    31741 accacaatac ccgccatcag cataccgccg aggaacgaca tatctttgcg ggtggtcagc
    31801 acatatgcag agcagcagaa gaacactaac gccgttccgc ccagtgccat agcgattacg
    31861 tcacccattc cggcagacag ataggtgttc agaattggtc cgaggatata acccagaaaa
    31921 ccggtaaagg cgaatgcgga gataatcccg gtcggcttat tcgccgtttt ataggtcagg
    31981 aacatcaaac catacatacc caccagcgtc agaatcagac ccggagatgg cagcatcagc
    32041 accgtactgg cagttgcggt aatcgccgaa aaggccagcg tcaggctcag cagaaaatag
    32101 gtattacgca gcaccttatg ggtgctaagc agtgatgtac ggtcatgtga agaactaaca
    32161 atacgatcca ttgagtcact ctctatgaca gatgtaatta attaagcagc ataatgataa
    32221 tgcgtaaggg cacccagaag ttttacccat ctttacgcat ttgatctgga acaggtttaa
    32281 cagcggatta tcaggtcatt aagcaaatat aacgccctga gaattccgac aggcaaaaga
    32341 aaaaggggtt agcatttagc taacccctta tcttatttgg cggaagcgca gagattcgaa
    32401 ctctggaacc ctttcgggtc gccggttttc aagaccggtg ccttcaaccg ctcggccaca
    32461 cttccggaat gacgcgcact ataaacatcc cgatgcggcg tgtaaacccc taatttgttt
    32521 gtttgcctga aaaatagcca aaagtgcatt gatagcgtga aataacagca gattgatcat
    32581 ttcatcacca tgaattcctt ctcttttact cgtttagcaa ccggctaaac atccccatcg
    32641 cccgggcaaa gaaaaatagg tccattttta tcgctaaaag ataaatccac acagtttgta
    32701 ttgttttgtg caaaagtttc actacgcttt attaacaata ctttctggcg acgtgtgcca
    32761 gtgcagaagg atgagctttc gttttcagca tctcacgtga agcgatggtt tgccttgcta
    32821 cagggacgtc gcttgccgac cataagcgcc cggtgtcctg ccggtgttgc taggagaaga
    32881 gacgtgcgat atgaataacg aggaaacatt ttaccaggcc atgcggcgtc agggcgttac
    32941 ccggcgcagc tttctcaaat attgtagtct ggctgccacg tcgctgggat taggcgcggg
    33001 aatggcacca aagattgcct gggcgctgga gaacaaaccg cgcattccgg tggtatggat
    33061 ccacggtctg gaatgtacct gctgtaccga atcttttatc cgctccgctc acccactggc
    33121 gaaggacgtc atcctttccc tgatttccct cgattacgac gataccctga tggctgccgc
    33181 cggaacccag gcggaagaag tctttgaaga catcatcacg caatataatg gcaaatatat
    33241 cctcgcagta gaaggtaatc cgccgctggg cgagcagggg atgttctgta tcagcagcgg
    33301 tcgaccgttt attgagaaac tcaaacgtgc cgctgccgga gccagcgcga ttatcgcctg
    33361 gggaacctgc gcgtcctggg gctgcgtgca ggccgcgcga cctaatccga cacaggcaac
    33421 gcctatcgac aaagtcatca ccgacaaacc cattatcaaa gtacctggct gcccgccgat
    33481 cccggatgtg atgagcgcca tcattactta catggtgacc tttgatcgct tgccagatgt
    33541 cgacagaatg ggtcgtccac tgatgtttta tggtcagcgt atccacgata aatgctatcg
    33601 ccgcgctcac ttcgacgccg gagagttcgt ccagagttgg gatgatgacg ctgcccgcaa
    33661 aggttattgc ctgtacaaaa tgggctgcaa agggcctacc acctataacg cctgttcctc
    33721 cacacgctgg aatgatggcg tttctttccc aatccagtct ggtcacggct gcctgggctg
    33781 tgcggaaaat ggtttctggg atcgcggttc gttctacagc cgcgtggtcg atattccgca
    33841 aatgggtact cattccaccg ccgataccgt cggtttaacc gcgcttggcg tggtggcagc
    33901 agctgttggt gtgcacgcag ttgccagcgc cgttgaccaa cgcagacgtc ataaccagca
    33961 acctacagaa accgaacatc agccaggcaa tgaggataaa caggcatgag cactcagtac
    34021 gaaactcagg gatacaccat caataatgcc ggacgccgcc tggtggtcga cccgattacg
    34081 cgcatcgaag gccacatgcg ctgcgaagtg aatattaacg atcagaatgt gatcaccaat
    34141 gccgtctcct gcggcaccat gtttcgcggg ctggagatca tcctgcaagg gcgcgacccg
    34201 cgcgatgcgt gggcgttcgt tgaacgtatc tgcggcgtct gtactggcgt acacgccctg
    34261 gcttcggttt acgccatcga agatgccatc ggtattaaag tgccggacaa cgccaatatc
    34321 atccgcaaca ttatgctggc aacgctctgg tgccacgatc atctggtgca cttctatcag
    34381 cttgccggga tggactggat cgacgtgtta gatgcgctga aagccgaccc gcgaaaaacc
    34441 tccgaactgg cgcaaagtct ctcctcttgg ccgaaatcat cccctggcta tttcttcgac
    34501 gtacaaaatc gcctgaagaa attcgttgaa ggcgggcagt tggggatctt ccgcaatggc
    34561 tactgggggc acccgcagta caaactgccg ccagaagcca acctgatggg ctttgcccac
    34621 tatctcgaag ctctcgattt ccaacgtgaa attgtcaaaa ttcacgcggt ctttggcggt
    34681 aaaaacccgc atccaaactg gattgtcggc gggatgcctt gcgccattaa cattgacgaa
    34741 agcggcgcgg tcggggcggt caatatggaa cgcctgaacc tggtgcagtc gattatcacc
    34801 cgcacggcag acttcattaa caacgtgatg atccccgacg ccttagtcat cgttcagttc
    34861 aacaaaccgt ggagcgaaat cggcacgggt ctttctgata aatgtgtcct cagctacggc
    34921 gcattcccgg atattgccaa cgactttggt gagaaaagtc tgctgatgcc tggcggcgcg
    34981 gtgattaacg gcgacttcaa caatgtgctg ccagtggatt tggttgatcc gcagcaggtg
    35041 caggagtttg tcgatcacgc ctggtatcgt tatcccaacg atcaggtcgg gcgtcatcca
    35101 ttcgatggca ttaccgaccc gtggtacaac cccggcgatg tcaaaggcag cgataccaac
    35161 attcagcagc tgaatgaaca ggaacgctac tcgtggatca aagcgccacg ctggcgcggt
    35221 aacgcgatgg aagtggggcc gctggcacgc acgttaatcg cttatcacaa aggcgatgct
    35281 gcgaccgttg agtcggtcga tcgcatgatg tcggcgttga acctgccgct ttccggtatc
    35341 cagtcaacgt tgggccgcat tttgtgccgc gcgcacgaag cgcagtgggc cgcaggtaag
    35401 ttgcagtatt tcttcgacaa gctgatgacc aacctgaaaa acggcaatct cgccactgct
    35461 tccactgaaa aatgggaacc tgcaacctgg ccgacagagt gccgtggcgt cggctttacc
    35521 gaagcgccgc gcggagcgtt aggccactgg gccgccattc gcgatggcaa gattgatctc
    35581 taccagtgcg tggtgccgac cacctggaac gccagcccgc gcgatcccaa agggcagatt
    35641 ggcgcttatg aagcggcgct gatgaacacc aaaatggcga tccccgagca accgctggag
    35701 atcctgcgta ctctgcacag ctttgatccg tgcctcgcct gttcaactca tgtgctgggc
    35761 gacgacggta gcgagctgat ctccgtgcag gtgcgttaac agcgaaggag aatcatcatg
    35821 caacagaaaa gcgacaacgt tgtcagccac tatgtctttg aagcaccagt tcgtatctgg
    35881 cactggctga cggtgttatg catggcggtg ttgatggtca ccggttactt tatcggcaag
    35941 ccgctacctt ccgtcagtgg cgaggcgacg tatctgttct atatgggtta catcaggtta
    36001 attcacttca gcgccgggat gatttttacc gtggtcttgc tgatgcggat ctactgggct
    36061 tttgttggca atcgttactc ccgcgagctg tttatcgtgc cggtatggcg taaaagctgg
    36121 tggcagggcg tgtggtatga aatccgctgg tatctgtttc tggcaaaacg tccgagtgcc
    36181 gatataggcc ataaccccat cgcccaggcg gcgatgttcg gctatttcct gatgtcggtc
    36241 tttatgatca tcaccggttt tgcgctgtac agcgaacaca gccagtacgc tatttttgcg
    36301 ccgttccgtt atgtggtgga atttttctac tggacgggtg gcaactcaat ggacattcac
    36361 agctggcatc ggctggggat gtggctgatt ggggcgtttg tgatcggtca tgtctacatg
    36421 gcgctgcgtg aagacatcat gtccgacgac acggtgatct ccaccatggt caacggctac
    36481 cgtagccaca aatttggcaa aataagtaac aaggagcgtt catgagcgag caacgcgtgg
    36541 tggtcatggg gctgggcaac ctgctgtggg ccgatgaagg cttcggcgtg cgggtggcgg
    36601 aacggctgta tgcccattac cactggcccg agtatgtgga gattgtcgat ggcggtactc
    36661 agggactgaa cttgctgggg tatgtcgaaa gcgccagcca tctgttgatt ctcgatgcca
    36721 ttgactacgg gctggaacct ggaacgctgc gaacctatgc cggagaacgc attccggcct
    36781 atctcagcgc gaagaaaatg agcctgcatc agaacagttt ctccgaagtg ctggcgctgg
    36841 cagatatccg cggacatctg ccagcacata ttgccctcgt cgggctgcaa cccgcgatgc
    36901 tcgacgacta cggcggcagc ctgagcgaac tggcacggga gcaactgccc gctgcggaac
    36961 aatcggcgct ggcgcagttg gctgcgtggg gaagtgtgcc gcaaccggct aatgaatcgc
    37021 gttgtctcaa ttatgactgt ctgtcgatgg aaaattacga aggcgttcgc ttgcgccagt
    37081 accggatgac acaggaggag cagggatgag caacgacacg ccatttgatg cgttgtggca
    37141 acgaatgctg gcgcgcggct ggacgccggt cagtgaatcc cgtcttgacg actggcttac
    37201 gcaagcgcca gacggcgtgg tgttattaag cagtgacccg aaacgcacgc cagaggtcag
    37261 cgataatccg gtaatgattg gcgaattact gcacgagttt cccgactata catggcaggt
    37321 ggcgattgct gaccttgagc agagcgaagc catcggcgat cgctttggcg cctttcgctt
    37381 tcctgccact ttagtgttta ccggcggaaa ctatcgcggc gtgctgaatg gtattcaccc
    37441 gtgggcggag ctgataaacc tgatgcgcgg gcttgtcgaa ccgcagcagg agcgtgcctc
    37501 atgagcgaaa cttttttcca tctgctgggg ccaggaacgc aaccgaacga tgacagtttc
    37561 agcatgaatc cactgccgat cacctgtcag gtgaatgatg aaccgagtat ggcggccttg
    37621 gagcaatgtg ctcacagccc gcaggtgatt gcgctgttaa acgagttaca acatcaacta
    37681 agcgaacgcc agccgccgtt gggcgaggtg ctggcagtcg atctgttaaa tctcaacgcc
    37741 gacgatcgtc actttatcaa tacgcttctc ggggaagggg aagtgtcagt gcgcattcag
    37801 caggctgacg acagtaaaag tgaaatacag gaggcgatct tctgcggatt atggagggtg
    37861 cgcagacgtc gcggcgaaaa gttgctggag gacaaactgg aggctggctg cgcgccgctg
    37921 gcgttgtggc aggcggcaac gcaaaatctc ttgccgacag attcgctgtt accgccgccc
    37981 attgatggcc tgatgaatgg cctaccgttg gcgcatgagt tactggcaca tgtacgtaac
    38041 cccgacgcgc agccgcacag cattaatctg acgcaattac ccatcagcga ggctgatcgg
    38101 ctttttctct cacgtctctg tgggccggga aatattcaga ttcgtaccat tggctatggc
    38161 gagagctata tcaacgccac ggggttacgc catgtctggc atttacgctg tacggacacc
    38221 ttaaaaggcc cgttactgga aagttatgaa atatgcccaa taccggaagt ggtgctggca
    38281 gcgccagaag atttggtcga ctctgcgcag cggcttagcg aggtatgtca gtggctggcg
    38341 gaagctgcac cgacgtaaaa agacggtaag tatcgctttc agtcttatga atatcgcaat
    38401 cggcgaatac ctctggtcgt agagtttcag gataaagagg gagatctaac attatcgggt
    38461 tatttttctc tcttcgccta caggagtgcg catgtgggat gtcattgatt tatcgcgctg
    38521 gcagtttgct ctgaccgcgc tgtatcactt tttatttgta ccccttacct tggggctgat
    38581 ttttttgctg gctatcatgg aaaccattta cgtggtcacc ggcaaaacaa tctaccgcga
    38641 tatgacgcgc ttctggggta agctcttcgg tatcaatttt gctcttggcg tggctaccgg
    38701 cctgaccatg gagtttcagt ttggtactaa ctggtcattc tattccaact atgtgggcga
    38761 tatttttggc gcaccactgg cgatggaagc attaatggcc ttcttcctcg aatccacctt
    38821 tgtcgggctg ttcttcttcg gctggcaacg gctgaataaa taccagcacc tgctggtgac
    38881 gtggctggtg gcgttcggtt caaatctctc tgcattgtgg atattgaatg ccaacggttg
    38941 gatgcaatac ccgaccggtg cgcattttga tatcgacacc ctgcgtatgg agatgaccag
    39001 cttcagcgag ctggtcttta acccggtcag ccaggtgaaa tttgtgcaca ccgtcatggc
    39061 gggctacgtg accggggcca tgtttattat ggcgatcagc gcctggtatt tactgcgcgg
    39121 acgggagcgc gatgtcgcat tacgctcgtt tgccatcggt tccgtcttcg gtactctggc
    39181 gattatcggt accctgcaac tcggagacag ttctgcgtat gaagtcgcgc aagtacaacc
    39241 ggtaaaactg gcggcgatgg aaggggagtg gcaaacggaa cctgctcctg caccgttcca
    39301 tgtggttgcc tggccggaac aggatcaaga gcgtaacgcc tttgccatca aaattcccgc
    39361 gctgctaggg atcctcgcca ctcactcatt agataaaccc gtgccgggtc tgaagaattt
    39421 gatggctgaa acctacccac gcttgcaacg cggacgtatg gcctggctgt taatgcagga
    39481 aatatctcaa ggcaatcgtg agccgcatgt gttgcaggca ttccgggagc tggaaggcga
    39541 ccttggttac ggcatgttgc tctcccgcta tgcgccggat atgaatcatg tcacagccgc
    39601 acagtaccag gcggcgatgc gtggcgcgat acctcaggtt gcgccggtat tctggagttt
    39661 ccgcatcatg gtgggctgtg gttccctgct gctactggtg atgctgattg tgcttgtcca
    39721 gacgctgcgt ggcaaaatcg accagcatcg ctgggtgctg aaaatggcgc tctggagttt
    39781 gccgttgccg tggattgcga ttgaagccgg gtggtttatg accgagtttg gtcgtcagcc
    39841 gtgggcgata caggacatct taccgacata ctccgcgcac tccgctttaa ccacaggaca
    39901 actggctttc tcactgatca tgatcgtagg gctttacacc ctgttcttaa tcgccgaagt
    39961 ctacctgatg cagaaatatg cccgtctggg gccgagcgcg atgcagagtg aacaaccgac
    40021 gcagcaacag gggtaaagga gaaaatcatg tttgattatg aaacattgcg cttcatctgg
    40081 tggctgctga ttggcgtgat cctggtggtc tttatgatct ccgacggatt tgacatgggg
    40141 atcggctgtt tgctgccgct ggtggcgagt aatgatgatg aacgccggat agtgattaat
    40201 agcgttggtg cgcactggga aggcaatcag gtctggttga tcctcgcagg tggggcatta
    40261 tttgccgcct ggcccagagt gtatgcagcg gcgttttccg gcttttatgt ggcgatgatc
    40321 ctggtgctgt gctcgctgtt cttccgtccg ctggcctttg attatcgcgg aaaaattgcc
    40381 gatgcacgct ggcgtaaaat gtgggatgcc ggtctggtca tcggcagtct ggtgccgccg
    40441 gtagtcttcg gtatcgcctt cggcaacttg ttgctcggcg tgccgtttgc cttcacaccg
    40501 caattacgcg tggagtatct cggcagcttc tggcaactgc tgacgccatt ccctttattg
    40561 tgtggtttgc tcagccttgg gatggtgatt ttgcaaggtg gcgtctggtt acaactgaaa
    40621 actgttggtg tgattcatct gcgttcacag ctggcgacta aacgcgctgc actgttggtg
    40681 atgctgtgct ttttgctggc aggttactgg ctgtgggtcg gtattgatgg ctttgtactg
    40741 ctcgcccagg atgctaacgg tccttccaat ccgttaatga aactggtggc agtgctacct
    40801 ggtgcctgga tgaataattt cgtcgaatcg cccgttttgt ggatcttccc gctgctgggg
    40861 ttcttctgcc cattgctgac ggtgatggcg atttatcgtg gtcgcccggg ttggggattt
    40921 ttgatggcat cattgatgca atttggcgtg attttctcgg caggcatcac gctgttcccc
    40981 tttgtcatgc cgtcaagtgt gagtccaatc tccagcctga cgttgtggga cagcacttcc
    41041 agtcagctga cgctgagcat tatgttggta atcgtgctga tatttttgcc cattgtgttg
    41101 ctctacactc tctggagcta ctacaaaatg tggggacgca tgacaacaga aactctccgc
    41161 cgtaacgaaa acgagttgta ctaaggagca ggaacatgtg gtatttactt tggttcgtcg
    41221 gcattttgtt gatgtgttcg ctctccaccc ttgtgttggt atggctggac ccgcgtctga
    41281 aaagttaacg aacgtaggcc tgatgcggcg cattagcatc gcatcaggca atcaataatg
    41341 ttagatatga aaagcggaaa catatcgatg aaagcgatct taatcccatt tttatctctt
    41401 ctgattccgt taaccccgca atctgcattc gctcagagtg agccggagct gaagctggaa
    41461 agtgtggtga ttgtcagtcg tcatggtgtg cgtgctccaa ccaaggccac gcaactgatg
    41521 caggatgtca ccccagacgc atggccaacc tggccggtaa aactgggttg gctgacaccg
    41581 cgcggtggtg agctaatcgc ctatctcgga cattaccaac gccagcgtct ggtagccgac
    41641 ggattgctgg cgaaaaaggg ctgcccgcag tctgctcagg tcgcgattat tgctgatgtc
    41701 gacgagcgta cccgtaaaac aggcgaagcc ttcgccgccg ggctggcacc tgactgtgca
    41761 ataaccgtac atacccaggc agatacgtcc agtcccgatc cgttatttaa tcctctaaaa
    41821 actggcgttt gccaactgga taacgcgaac gtgactgacg cgatcctctg cagggcagga
    41881 gggtcaattg ctgactttac cgggcatcgg caaacggcgt ttcgcgaact ggaacgggtg
    41941 cttaatttcc cgcaatcaaa cttgtgcctt aaccgtgaga aacaggacga aagctgttca
    42001 ttaacgcagg cattaccatc ggaactcaag gtgagcgccg acaatgtctc attaaccggt
    42061 gcggtaagcc tcgcatcaat gctgacggag atatttctcc tgcaacaggc acaaggaatg
    42121 ccggagccgg ggtggggaag gatcaccgat tcacaccagt ggaacacctt gctaagtttg
    42181 cataacgcgc aattttattt actacaacgc acgccggagg ttgcccgcag tcgcgccacc
    42241 ccgttattgg atttgatcat ggcagcgttg acgccccatc caccgcaaaa acaggcgtat
    42301 ggtgtgacat tacccacttc agtgctgttt attgccggac acgatactaa tctggcaaat
    42361 ctcggcggcg cactggagct caactggacg cttccaggtc agccggataa cacgccgcca
    42421 ggtggtgaac tggtgtttga acgctggcgt cggctaagcg ataacagcca gtggattcag
    42481 gtttcgctgg tcttccagac tttacagcag atgcgtgata aaacgccgct gtcattaaat
    42541 acgccgcccg gagaggtgaa actgaccctg gcaggatgtg aagagcgaaa tgcgcagggc
    42601 atgtgttcgt tggcaggttt tacgcaaatc gtgaatgaag cacgcatacc ggcgtgcagt
    42661 ttgtaatgca taaaaaagag cattcagtta cctgaatgct ctgaggctga tgacaaacga
    42721 agaactgtct aatgcgtaga ccggaaaagg cgttcacgcc gcatccggtc actttcagtt
    42781 ttactctttc tcggagtaac tataaccata atagttatag ccgtaactgt aagcggtgct
    42841 ggcgcgttta atcacaccat tgaggattgc gcctttaata ttaacgccag cctgttccag
    42901 acgctgcatt gacaagctca cctctttggc ggtgttcaag ccaaaacgcg caaccagcag
    42961 gctggtgccg acagagcgtc ccacgaccgc agcatcgctc accgccagca tcggcggcgt
    43021 atcgacaatc accagatcgt aatggtcgtt cgcccattcc agtaattgac gcatccgatc
    43081 gcgcatcagc agttcagacg ggttaggtgg cacctgaccg cgagtaatca catcaaagcc
    43141 tcctttgcca aaatgctgga tcactttgtt gagctcatct ttacctgcca gatattccga
    43201 caagccatgt tcattactca cggtaaacag gttatgcgaa taaccacggc gtaagtcggc
    43261 atcaataaat aacacttttt gatcggactg ggcgatcacc gctgccagag ttgaactgac
    43321 aaacgtttta ccactgtctg gcgtcgcacc ggtgatcatc agaatgttat tctcagtctc
    43381 catcatagcg aaatgcagac tggttcgtag cgcacgtacg gcttccacag cagaatccgc
    43441 cgggttatcc accgccagga aggggatatt tttagtacga tggcgctgct gattagaaaa
    43501 taaatttttc ttacgcagac gggtgcgttt atccagccac tcggacattg ggatagtgga
    43561 ataaacgctg atgccgtgct cttccagttg ttccggggct tctacaccac gacgcaacat
    43621 cgcacgcgcc agcacggcac tcaccgaaat aaacaggcca agaataaaac caagcaccac
    43681 attcaacgct tttttcggtt tcactggctg cggctgagtg actgccgggt cgataatccg
    43741 cacgttacca atggcactgg atttcgaaat actcaactcc tgctggcggt taagtaattg
    43801 cagatatacc gcacggcccg cttctacgtc acgacttaaa cgcaacactt cctgttgggt
    43861 ggaaggcatt gccgataccc gcttattcag gcgtttgcgt tcttgctcca gcgtctggcg
    43921 tttttccagc agcgcacgat aagttgggtg atctttctta tacagcaggg agatctctgc
    43981 ctcgcggaag gtcagctcat tgagttgatt atcaacgttc acaatctgct caagaacggc
    44041 tttggcttcc aggttaaggt caaccgaatc gcgctgctgg cgataaacgt tgagtttttc
    44101 ttccgcctgg tccagctcgc tgcgcacttc aggtaactgg cgctgtaaga attcaaggct
    44161 ttgtgaatcc tgcgccgcct ggcgagcgat attctgttgc aaatagttgt tagcgatgct
    44221 gttcagaata cgagtaatca actggggatc atcaccagtc atggtaagtt ccagcatccc
    44281 gctttcttta ctgcgttcgc taacggtaaa ggtttcctgc aatgcgttaa tcgcttccag
    44341 ttcggtacgc tggctcagga caaactgtgt tcctggtttg gccttaatgt ccgcgatagt
    44401 cagcgcaacg ccatcttttt ccagacgctg gccgaccata ccattgacgg tgaactcttc
    44461 accttccagt gtatagtggc cgttttcccc aaccgtgagt gtcagttgct gatcctgacc
    44521 attcagttgt ggaatatgca tccagctgat cgccagctca cctggttttt ctttggttaa
    44581 tctcgcccag ccgcgaccca caatcggaaa atacttctgc tcaactatgt cgcgcagatt
    44641 cagttcagca atggttttac cgagaatcat gcgcgattgc agcagttgga tctccggtgc
    44701 agactcgggc gatgagttag ggatcatatc gctcaggccg ctgagaatgg cgttgccctg
    44761 tttttgctca acctggacca gagtatctgc ctgataaatt ggtgtgctta acagcgatca
    44821 gcgtgaataa cgcggtcacg ctgataataa acttacggtg atcccataac tcgccgacca
    44881 gacgaagcag atcgatctca ttttcctgag tgctgcctgg tggcgtattc atatttttag
    44941 ttgtcatcgt attttataaa ctcaaattac cggctgaggc gcttcgccca ttcctgactg
    45001 gcgcgctcca acataccgta gacatgttca aatgcgtcct gacttttacg atagggatcc
    45061 gggatctctt tctgttccag ccattgccca aacagcattg tcttcccgcg gacctcggga
    45121 gcaattgcgg taacctgagc aatatgttcc gactccattg ccagaatcag atcgtaattt
    45181 cgtgccatct cagcagtgag cttacgtccg gcatgtcctt caagagatac gccatgatta
    45241 gcggcaacat ctgccgctgt cgcatctgca gggtgtttta ccagaccatg aacacctgcc
    45301 gatttgactt ttacgcccgg cagacgttta cgcagcaagc gctcgccaat gggcgaacgg
    45361 caaatgttgc cggtacaaac cactaggatt gagttaaatt ttagttgggc catgtttgaa
    45421 tccaccgtga agtctctgtc aggtcatgca ccccggaaat cgtcggtacc agcagagaaa
    45481 tcacacggtt ccaacgagcc agcggcgcgg tagtgacata aacaatgtcg taaggttcaa
    45541 gctggaattc cgttccgagt atcattgccg atgcgtcctt cgcattcagc tgataaatat
    45601 tggcgatttt gccattctgc ttgttttgcg ttgcacgaat aacaaaaatc cccgtcgcat
    45661 cagcgacatc ctgattcata ccctcagcgt tacccagcgc ttccgccagg gtcataccgc
    45721 tgcggtccat tttcagcgtg ctctgtttac ccacttcacc catcacgaag actttcagat
    45781 catcgttgcg cggaataaat aaaatatcgc ccgggtgcag cagtttgttc tggcgcaagt
    45841 cgccacgctg catcagcgca tagaggttca ctttggtctt tacgccattc tgggtgagca
    45901 ccacgttgcg ccagtctgca tctgcggtca gaccgcccgc ggcgttaatc gcatccatga
    45961 tggtcagagg aatattggta atcggctgct gacccgattt gctgacttcg ccggtgacgt
    46021 acgctttttg cgatcggaac gccgcgacgc tgacatcgac ttgcgggctt tcaatgactg
    46081 aatcgagacg cgctgtaatc tcgttgcgta cctgggtaag tgttttcccg gccactttca
    46141 ggcgaccaat ataggggtag aaaatcgcac catcggcgtt aacccagttg ccggtatcac
    46201 tggcgctacg gtattgccct gcgggggtgg ttagttccgg gtgatcccag accgtaacca
    46261 tcaacacatc accaatcccg atacgatatt catagtttgc gatttgctgc tccagctccg
    46321 ggttagcctg cgacataatc ggtttggcgc gcagctgatc aatcaatcct ggcgtgacgg
    46381 gatagatatt gaccattttg tccaggtcat actgattgtc aggtagttca atgacatcct
    46441 tgttactggt cgataaattc tgaccaggaa caagggtaca tgctgtcaaa gacagaacag
    46501 ccaatgtcag taccgaaaac ttaaaaatgt tctttttcat tggtttatat ttatcagtca
    46561 atgtaatgtt atgaattacg tcaggttatt gtccaaccgg tacttctcgc tctgccgtca
    46621 ttggatcgag ctggtatttg cgtgacagca tttgtccacc atcacgcgtc aatggtgtcc
    46681 acgaaaccac cgcgcggttg cggttaggtc caatggtcat caagtcgaac ggaatcgaga
    46741 tataaaaacc tttactaaag ccgccttcgc cgtagtcatc tttagatacg ttactgattg
    46801 ccgcccatac ccctaccgcc acgccgctgt caaagcgttt ggcgacgtcg atcgttgcgc
    46861 ctttatcttt tgccagatat tgcccaatgc taagtttcat cagtacgcca ttgagcgtcg
    46921 gcgggttcca gtaagccgtc acgaaacctg tgggcgtgga ataatcggtg aagcgcatca
    46981 tgttgtccca gtcacgctgc ttcacgtagt taacgtccac acccagcgcc cagctggcat
    47041 ctagcgggcg ataaagaagc tcggaaccga caccggcata catcgtttcc agataaccgc
    47101 catacacctg gccatagaaa ccattgccta agtcggcaaa gtaattcgct tgcaagttgt
    47161 tgagataaac gtcattgcga acgtaatcac ggatatgcgt gcgcacgcgg ggcagggtag
    47221 agtccgcggg taacagtgaa gacttaaact tgtcgtagtt gttgtaaata ttggtgaaaa
    47281 taccgccatc aagcagcagg tggtcggtaa accagtagcg ggcgctggac atcagcccca
    47341 gctggaacat atagaaatct tccgggccgc ccagcgactg tgaaagtgtt gggttgaaag
    47401 agtagctaaa gcgatcttca cgaatacggt agccccgacc aaaggcagaa agatcttctg
    47461 cttcaacacg ttgttgttgc agttgctctg atttaccagg cgctgtgccg gccagctgtt
    47521 tgcgcaggct ggctacgtcg gtttcggtag tcaccatcgc catatgctcg cgcttttgcg
    47581 tcacgctaat cttctcaacg ccttgcggca ggttattcac cagaatccgg ttggcacgat
    47641 cgaccgcttc gcgagagtca cggtatttgt attgctgacc agacatatac agcgtcttat
    47701 cgcgtagctg aatttctggc gcgtcaaagc ccgcgttata cttcagggcg gtaagttggt
    47761 ttgctaccgt ggtgtactgc aaaccttcag actctggcgc aggctgccag gccggttttg
    47821 gcgtatcgcg tagggcaggg cgcagatcgt tgaaattggt ccgtaaagtg aaaccaaaca
    47881 tcaacgtgtt tccgcgttca taactgaggt tgaggtctgc ccagctggca gcacgataaa
    47941 ctgcaccgac gttgaaatgg cttgcctgag gcagtttgcc agcgaaatca ttctggtaat
    48001 tgttgccgtc gtattcgagt ttcagacgca ggggattcca cggcgtttga tactcaatgc
    48061 cgccaaagat ggaagccggg ccacgaaaga tatcgctaaa gctgatatcg cccgcatcgt
    48121 gagactctgc gcgatgacaa tatttatcgc tcacccggca aaacgggttg gtaatattgc
    48181 ccgcattacc ggcgtagccc catgccatcc cgagggtgaa atcaaatggc cccgcttgct
    48241 tgctggccac cagatattca ccgtcaaaca aacccgtacc agcaatatca cgtttaccaa
    48301 acgccacttg cggtagccag taaccttctt cccacaggcg aagtttaaaa tcgaatgatt
    48361 tgtctttgta tgactgatcg ccgctgaaat cttcccactg gctatatttg cgtgtgcgca
    48421 catccgtata acgaatggtg ccttccagcc atgggaacag cgccacggag gtggagtaga
    48481 accggtattg atcgttatcc cggtagttga cgctgaattc accttccggt gcgatgcgcg
    48541 catttggcat ctgcaacaat cctgtgccgc caaagtctga ttgcgacgaa cccagcggat
    48601 ccgggtaggt taatacttca gcatgacagg cactggagac ggcaatggcc aggcagctta
    48661 aaagataaga attcttcttc atcagtcagg aacacgttgc gttaaaacag acacaatctg
    48721 gtcatttaaa tcagcatatt tttcgggcag tacatgcgca gaaaagccca accaaagctg
    48781 gctgccaggc ggtggttcca catgtctttt attccacagg gcaacagggg cgacgacagt
    48841 ctcaccctca ggcgtaatca ccataacgtt attcttgtcc gcgcctgcaa ggcgggggtg
    48901 atcttgcaga taatcggtaa cgctacggcc tgcctgccag ggcaattgcc cagccccaga
    48961 aaccgcaccc agcagagtaa tagtcaccgg acgctgtacg gtatagagcg tatagtcccc
    49021 gaccaacggg ggattgctgt tctcatccac tcgtacaaaa tcagggtcca gtttgaccgg
    49081 taagcgacca gtgatattga gattgagtaa ctgctggcgc acggatttaa tcgttgccgc
    49141 cacatcgtcg tcagcttctg cttcccagga agcaagctgt gccatgacat gttggtagtc
    49201 tttaagcgct ttagctttcg ccgcagagtc ggtcagcaag gcaccaggcc accagaggcg
    49261 atcgcgcagt tgcggttgtg tcaccaactg gacaacattt tccacggggc caacggaaag
    49321 tgtctgttgt tcgctaggca gataaatagt caccgttcct tgcgcaaagg catggggtgt
    49381 cattacgtaa agtacgctgg caatgaaata cgactgtaat ttattcatgg tgcgggttta
    49441 agaaacgtca tttctacggg aataacccct gcgcctaaca tttgccgact ttgacgaacc
    49501 tggccagtag ccgaatcgac ccagaatgtg ttttgccact gtttttctgg cagcgtggaa
    49561 gtcacctctt cctgccagac attacacagc acattgtgac ccgcaatatt cagcgtctct
    49621 aatccagcaa aggaaaaagt agaacttagg gtcgcagagc gaaaatggtt gtcttcactc
    49681 cagagaatat cgcgcgtcca gcggctgcca tcttttattg ccagcgcgtt acgcagaggg
    49741 tcccgcccgg gatttgtcac ttccagcaga ttattattaa gtttgacggt ctttaaaaga
    49801 cgtccattgt gggtaaccag catggcgtta tcctgggata accatttgct ttgttcttgt
    49861 tctatatatc ccagtaccac aaagattcgt tgcccttcat taaggcgtaa atacatcgtg
    49921 gaataaggca acgcctggat ctgctgatcc gctacagtga tatcctgatt atcgaaaagg
    49981 ctggcgcgaa atgtatcgac catactttgc tggctgtgcg tacatcccgc aagaaatagt
    50041 gcgaaaatcg ataaaataag agggcgcacc tttaatcctt gaaaggttaa ttaaaaaaat
    50101 gcaaatctct aaagcccggt acgaatatcg caaccgggta ttatttaact tgaatttcga
    50161 cgcgtttaag cagaattagc gcgtagtgga cgtggtggtt gaggtgttat gagaaccgcc
    50221 atcgccactg tcatttgctg caactactgc caacgtcgcc acaccaacag ccgttgccac
    50281 accgacaact gtcgccgtgc cgacagcgcc agcagccacg ccactagtgg tagccgcagt
    50341 cgtggttgca cctgtagtgg tcgttgtggt gccagtagta cccgtcgttg cctcggtccc
    50401 gtttgctgct tcaggggcag caaacgcagc tggcgtcgtc aacatgaagg ccataaggat
    50461 tgcagaaagc ttgtgtttca taacttttcc tttattcatc gcatggacaa tacgtacgct
    50521 tgagatcagg attgtctact ttgagtataa catccttgtg aaatctccct ttcctcatac
    50581 cgctagcact atatactgcg ggtgggaaag gcgcgcagag ggaaatataa gattgttaac
    50641 gatttccctc tggaaatacg acttgtgagc ataagataaa tcttagaaaa atacgaatta
    50701 aagataagaa aattgttacg aaagctatta attattgttg gtaatatagt ttcaagtggt
    50761 actataactg gctattaaat ggcatttata actattaggt gcagagatat tcgcttaaat
    50821 ggattagttt cttggtggaa aagtaactga tgttataatg tatggtgttg actattgaac
    50881 tattccaata aaaccagtca gctttaaaca agcagcgtca tattaagaga gataaacatt
    50941 tgccgctgtt ggtcctcgca ggccatttac gcggcaaaat tccacgcgta atcctggtat
    51001 aagcacttct gcgtcgcggg gagtgaatgc ggaaatatgg acctggactt ctttacgacc
    51061 gtcggagggg ataatgaatc ctttgccgct tttgcgatca aaggttttga caattcctgt
    51121 cattttacgg gacaaacaaa ttccttactg aaaatactgc gctgcactat acggggttaa
    51181 taaaataaag ccagcgatat ttaagaccgc cggacggcta aaataaaatt tgcttaatct
    51241 caattatcat gccttaatag ctgcgtcggt ttgaaagaca gacagcatac aaagtagttt
    51301 actaaagcag ttctcattat caggcattat ccccttcttt tgagtctctc tcctgaacac
    51361 taagtggttt ctgtattaaa gccctgtttg ccgaaaggcc caaaatgaag gaagtaaaat
    51421 atgtctaata aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt
    51481 atcactcctg atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat
    51541 gaattccgca cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt
    51601 ggccccgcgg cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca
    51661 tttccgctgt ccatgttgtc atggttcaca gtaccgtaca tcggcattcg atgtgacgga
    51721 gcgaaaccct ttgggcgcta agtgtatttt ttgtaaatcg actatgatca cctttgataa
    51781 cgtcgcgctg caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc
    51841 tccctcgtgg agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa
    51901 gcgataacct gttgattatt gaatctttcg gggagatggc ttataacatt ccttacctga
    51961 ccagggtacc gggaaccaac accttactgg cgtgttgctg tctttcaaga ccagacgagg
    52021 ttaacagtga atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc
    52081 gcgcaaaaaa tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc
    52141 atcgcacgag aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga
    52201 ttttgtgaac atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat
    52261 cattggcgct tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac
    52321 tcccgcatac attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat
    52381 taaccagcca gacaacgcaa aggctttttt atttgctgca ggtttttgca gtgaaaaggt
    52441 caatgttgag gctaccggac atgactctac gcaagtcata cagccggtac attccactgt
    52501 tcgtacctga attaatttat ctaccgggat ccgtgatggg cacttttttg cgcatttgcc
    52561 acaatcgata caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac
    52621 gcccatcagc gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct
    52681 ggcaatcagc aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc
    52741 gagcatttta acgtccatca ccacgctata gggcgacaac ataaaatagt gaatcgcctg
    52801 agcgggcatc aataacgcga tatagagaaa taaactcaac agcaaatact tcacgccgcg
    52861 cagaggaata tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag
    52921 atcgccgatt aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt
    52981 gagtaataga ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga
    53041 cggtaactga cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca
    53101 gccttcgata cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt cgtaatagcg
    53161 cacccaatac cagaacgtga tggcaatata aatattcatt gccaatagta agaattgcgt
    53221 cgctttacgc caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt
    53281 gcctggccgc cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta
    53341 agttgcatac caaatgccac tattctagtt attcttaact ggctgatatt gattcaaatc
    53401 gcgttcaggt ctttcttatt caatcatgct tccagagcgg caacactgcg tgtaatttct
    53461 tcgtgtggaa ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg
    53521 ctacgcattc ccagactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc
    53581 tcgctgtcct ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt
    53641 ttaaataatg ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg
    53701 agttgcgaga catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt
    53761 aactgaccga gcacttcacg cggcaccggt ttagggataa tcccgcgaaa tagtgaactg
    53821 gtacgctggc gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac
    53881 ggatattgct gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg cagatcaaaa
    53941 tccaccagtg cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca
    54001 ttgccaatag caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg
    54061 gttagcgggt tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg
    54121 gctgtttttg gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca aaaacagctt
    54181 ccaacctccg gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta
    54241 ctgatagtca gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg
    54301 aagatttctg ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc
    54361 cattgctcgc catcagtgcg actacgcagg ataatgtgcc cttcgtcagt aaaacgcagg
    54421 gcattgctca ataggttggt aatgacctga cgaatacgtc gtggatcgcc cattaacgcg
    54481 ctcggtacat catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg
    54541 ctcattaatt gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgact
    54601 gaaacatttt tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc
    54661 agcaacgatt cgccagagtc ggtaatcgcc cgcaaatcat cacgctgggc gttaagtgcg
    54721 gggttatctg ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca
    54781 tggctcatcg ccgccagaaa cgccgatttt gcctagctgg ctttttctgc ttccgcccgt
    54841 gcctgtcggt gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc
    54901 gccagctgtt cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc
    54961 cgcccgatgg tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca
    55021 ccgtccagca gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gactgagcga
    55081 taaaccacgc gccagaggat cagaatcagt gcgcaaagtg aaaccatccc cagcaataac
    55141 aggctgtatt gcccgcgtgc actggctttt tccagatgtg ccagtccatg ctgattgcgc
    55201 agctcagtgg tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata
    55261 ttattttggg cgagagtttg caggtgattg ctgatttcac tgtcctgctg gaacagcgcc
    55321 aacagatcgc tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaactccc
    55381 ggatcttcaa tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt
    55441 tccagcgttg gtgcattttt ctggagctgc tccagcccca gattcatcac catttgctgc
    55501 acccgcagag cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca
    55561 atcagccgat cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa
    55621 atcccggcct gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgcgccagg
    55681 cgtgcgatct catcggcggc agctatctgc tgactgagtt gccgttgttg ctggcgtagt
    55741 tgcagacgcc gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc
    55801 tgttgttcaa tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta
    55861 atcttcaggc tttgtgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg
    55921 gcactggtca ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc
    55981 gccatattca tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag
    56041 cgcaggttgt accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag
    56101 cccatccaga gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag
    56161 gttgagtcgc aaagtggaat gcatctagca taaagcctta ttattgatga ggctatcatg
    56221 cgcgtactgc tatttttact tctttccctt ttcatgttgt cggcattttc ggctgataac
    56281 ctgttgcgct ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc
    56341 aaacgcgtat ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg
    56401 ttgaactatg gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag
    56461 gcaggcggct acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg
    56521 ggcgcagagg ccattttgct cggtagtagc acgacgtcat ttcccgacct gcaaaagcag
    56581 gtagcaaatc tgccggtgat cgaactggta aatgctcttg atgatcccca ggtgaaaagc
    56641 cgcgttggtg taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg
    56701 gcgcacggta acccactgaa cgtgctgctg atgcccggac ccgataacgc cgggggcagt
    56761 aaggagatgg tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat
    56821 attgcgcttg gtgataacga tattgaaatc cagcgcaacc tgttgcagga gatgctggaa
    56881 cgccacccag aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt
    56941 gaagggcgta acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg
    57001 tatcgcgggc tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag
    57061 ggggaactgg cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat
    57121 gtcagcccac cgattttagt tctgacgccg aaaaatgccg accgagaaca tatccgccgc
    57181 tcgctgtcac cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag
    57241 aaataacctt caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga
    57301 cgaattaaca catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata
    57361 cgcagcagac gctcacggct gagaatttcg cccggattag tcacaaatgc caccaacatt
    57421 tcatactctg cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac
    57481 acattcaggc aataacccgc aaagcgatag cagtagtcct gagtgtgcgg ttgagcttgt
    57541 cgcgcgaggt cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc
    57601 agcggtttgg tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga
    57661 tcgctgcgtc cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt
    57721 aacatcaggc cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga
    57781 ttctgcataa tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc
    57841 tgagtgaagt aggattgtaa tcgcgcctgg gtaaccggct catcttcaac aataacaatg
    57901 tgatgtggca tcagagggtt ttactcattc tgttcatatc tgttcatatt ctgccgtaag
    57961 ccgttcatcc tgaccagtgc cgctgttcat atttgctcat taagatcgct tcactaaacc
    58021 ataattctac aggggttatt atgcggaaac tctggaacgc gctacgccga cccagtgctc
    58081 gttggtcggt actggcgctg gtcgcaattg ggattgtgat tggcattgcg ctgattgtat
    58141 tgccacacgt tgggatcaaa gtcaccagca caaccgaatt ttgtgtcagt tgccacagta
    58201 tgcaaccggt gtatgaagaa tataaacagt cggtgcattt ccagaacgcc tccggcgtgc
    58261 gagctgaatg ccatgactgt catatcccgc cggatattcc aggcatggtg aagcgcaaac
    58321 tggaagcgag caacgacatt taccagacct ttattgccca ctccattgat acacctgaaa
    58381 aattcgaagc caaacgcgcg gaacttgccg agcgtgaatg ggcgcgaatg aaagaaaaca
    58441 actcggcaac ctgtcgctcc tgccataact acgatgcgat ggatcatgcc aggcagcatc
    58501 ctgaagcggc acgtcagatg aaggtggcag cgaaagataa tcaatcctgc atcgactgtc
    58561 ataaaggtat tgcccaccag ttaccggata tgagtagcgg cttccgtaag cagttcgatg
    58621 agctgcgcgc cagtgctaat gacagtggtg acacgctgta ctctattgat attaagccaa
    58681 tttatgcggc gaaaggcgat aaagaagcct ctggttctct gctgcctgct tcggaagtga
    58741 aagtccttaa acgtgacggc gactggctgc aaattgaaat taccggctgg acggaaagcg
    58801 ccggacgtca gcgtgtactc acccaattcc caggtaaacg catctttgtt gcctcgattc
    58861 gtggtgatgt gcagcagcag gtgaaaacgc tggagaaaac caccgttgcc gacaccaata
    58921 ccgagtggag caagttgcag gccaccgcgt ggatgaagaa aggcgacatg gtgaacgata
    58981 tcaaaccgat ctgggcctat gcggattcgt tgtacaacgg cacctgtaac cagtgccacg
    59041 gcgcaccgga aatcgcccac tttgacgcta acggttggat cggcacgctc aacggcatga
    59101 ttggctttac cagtctcgat aaacgtgaag aacgcacttt gttgaaatat ctgcaaatga
    59161 atgcgtctga caccgcaggt aaggctcacg gcgataagaa ggaagaaaaa taatgaacaa
    59221 taacgatctc tttcaggcat cacgtcggcg ttttctggca caactcggcg gcttaaccgt
    59281 cgccgggatg ctggggccgt cattgttaac gccgcgccgt gcaactgcgg cgcaagcggc
    59341 gactgaggct gtcatctcga aagagggcat tcttaccggg tcgcactggg gggctatccg
    59401 cgcgacggtg aaggatggtc gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc
    59461 gtcgaaaatg atcgccggat tgccggatca cgtacacaac gcggcacgta ttcgttatcc
    59521 gatggtacgc gtggactggc tgcgtaagcg ccatctgagc gacacctccc agcgcggtga
    59581 taaccgtttt gtgcgcgtga gctgggatga agccctcgac atgttctatg aagaactgga
    59641 acgcgtacag aaaactcacg ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac
    59701 ggggatgttc cataacgctt cggggatgct ggcgaaagct attgccttgc atggtaatag
    59761 cgttggtacg ggcggagatt actctaccgg tgctgcgcag gtgatcctgc cgcgcgtagt
    59821 cggttcaatg gaagtgtatg aacagcaaac ctcctggccg ctggtattgc agaacagcaa
    59881 aaccattgtg ctgtggggct ccgatttgct gaaaaaccag caagcgaact ggtggtgccc
    59941 ggatcacgat gtttatgaat attacgagca gctaaaagcg aaagtcgccg ccggtgaagt
    60001 tgaggtcatc agcatcgatc cggttgtcac atccacccat gagtatctgg ggcgcgagca
    60061 tgtgaagcac attgcggtta acccgcaaac tgacgtgccg ctgcaactgg cgctggcaca
    60121 tacgctgtac agtgaaaacc tgtacgacaa aaacttcctc gctaactact gtgtgggttt
    60181 tgagcagttc ctgccgtatc tgctgggtga gaaagacggt cagccgaaag atgccgcatg
    60241 ggctgaaaaa ctgaccggca ttgatgccga aaccattcgt gggctggcgc ggcagatggc
    60301 ggcgaacaga acgcaaatta ttgctggctg gtgcgtgcag cgtatgcagc acggtgaaca
    60361 gtgggcgtgg atgattgtgg ttctggcggc gatgctgggg caaattggcc tgccaggtgg
    60421 tggttttggt tttggctggc actacaacgg cgcaggcacg ccggggcgta aaggcgttat
    60481 tctgagtggt ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gtgactacaa
    60541 aggctacagc agcactattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa
    60601 agtgatcaac tggaacggta aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc
    60661 cggaactaac ccattccatc gccatcagca gatcaaccgc attattgaag gctggcgcaa
    60721 gctggaaacg gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat
    60781 cgtgctgcct gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc
    60841 caaccgtggc attatcgcca tgaaacaggt ggtgccgccg cagttcgagg cgcgcaacga
    60901 ctttgatatt ttccgcgagc tgtgccgtcg ctttaatcgt gaagaagcct ttaccgaagg
    60961 gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg
    61021 acgcggcgtt catctgccag cgtttgatga cttctggaat aacaaagagt acgtcgagtt
    61081 tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc
    61141 gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgccg atatgaacta
    61201 cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc
    61261 cggctcgcag aagtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc
    61321 gcagttatgt gagtcggaaa cgctgcgtca gcaatatacg gtagcgggta aagagccagt
    61381 gttcattaac ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt
    61441 ctttaacgct cgcggtcagg tgttggcagg agcagtagtt tctgaccgct atgcacccgg
    61501 cgtggcgcga attcacgaag gggcatggta cgatccagat aaaggcggcg agcctggtgc
    61561 gctgtgcaaa tacggtaacc ccaacgtgct gaccatcgac atcggtactt cgcagctggc
    61621 gcaggcgacc agtgcgcaca ctacgctggt ggaaattgag aagtacaatg gaacagtgga
    61681 gcaggtgacg gcgtttaacg gccccgtgga gatggtggcg cagtgcgaat atgttcccgc
    61741 gtcgcaggtg aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg
    61801 ctggcgcagt tgttctcccg tgaactggac gatgaacaac tgacgcaaat cgccagtgcg
    61861 cagatggctg aatggttttc gttgctgaaa agcgaaccgc cgctcactgc ggcggtgaat
    61921 gatctggaaa acagtattgc cacgctgaca gttcgtgacg atgcccgtct ggaactggcc
    61981 gctgactttt gcggcctgtt tctgatgacc gacaaacaag tggcgctgcc gtatgcatcg
    62041 gcctacaaac aggacgagca agagattaaa cgcttgttag ttgaggcagg gatggaaacc
    62101 agtggcaatt tcaacgaacc ggcagatcat ctggcgatct atctcgagtt gttgagtcat
    62161 ctgcattttt cgctgggaga ggggaccgtt cctgcgcgaa gaatcgacag tttgcggcaa
    62221 aaaacactga cggcgctgcg gcaatggtta ccagagtttg ctgcgctttg tcgacagtat
    62281 gacagctttg gtttttacgc ggcactaagc cagttattgc tggtgttagt ggagtgcgac
    62341 caccaaaaca gataacgtcg tttatgcgcc tgaaaagacg cgtttagcgt cgcatcaggc
    62401 attatggcgc agttgccgga tgcgtcatca catccggcaa acacacatca cggatgagct
    62461 acaaaccggg aaagccgctg gcgtagcagg cggttttcct gcttcaggtg cgcaatatca
    62521 tccattaacg tcagcgccac cgcgatcccc ggccagtcca gagccagttc atgacgcagg
    62581 cgtaccgcgc gttgcaccac aatggcggca tggtcgtcaa atacccaggt tgtttcctgg
    62641 atctcacgcg gttcaaccac ccccaaaccg acaatttcat tcaactcctc ttcagagatg
    62701 ccggtatgca ggcaaaattc ggtaatagta aaagtcaccg taacattagc cattatgctt
    62761 tcccccaatc tttacgtgga tcaaaagacg actgggcgtc tgccagttgc tgccacagcg
    62821 cggcagtgtt ttcatccggt ttcggcggca tcacgatttt cagtaccgca tacagatcgc
    62881 cggtctgttt tttgctcacc agacctttgc ctttaacgcg caatcgttgc ccggcctggc
    62941 tgcctggcgg gatagtcagc aaaatgcttt ctttcagtgt tggaacggtg actttagtac
    63001 ccagcgccgc ttcccacggg ctaaccggca ccacaatttc cagatcctgg ccgacaatat
    63061 cgaacagcgg atgtggcgca atatgaatca ccagccacaa atcgccattt ggaccgccgt
    63121 tttcgcccgg cgccccctgg cctttcaggc ggatgcgttg accattgccg acgcctgccg
    63181 ggatcttcac attcagcgtt ttcgggattt cctgttcgat catgccaaag gcgttataaa
    63241 ccggcaggtt atagctgatg gtacgcttat gctcagtaag cgtttcttcg aggaataccg
    63301 ccacttcgat ttcaatatcg tggccgcgtg cggcggggcg ttgacggctc tggcgggcat
    63361 gctgaccgaa aattgacgag aagatatcgt caaaatcttc ggcattaaaa ctctgaccgt
    63421 cgccatgatg gaactgacgg ttaaattgcg gatcgttgcg atgttgccac atctgatcat
    63481 actcagcgcg acgttgttca tcacttaaca cttcccaggc ttcagcgacc tctttgaagc
    63541 gggcttcggc atccggttct ttgctgacat caggatggta tttacgggcc agtcgacgat
    63601 aggcggtctt gattgtcttg agatcgtccg tcggtttcac gcccatgatg gcgtaataat
    63661 cctttaattc catagcgtta tctcgcgtaa atcaacacaa attgaaggaa cccctgtaag
    63721 gtaactccta taagtgtagg gtaatcctca aaatttcata tgccaacaca gaatatgtta
    63781 ttgaaatcat cgcggagagg aggtcgccat caagatgggt tgctgaacat attttaaaca
    63841 ggtgaaaaag ggtgagcgat ttttgatagt tgaaccaggc actttaagtt
//
LOCUS       DADLSA010000006        62422 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.006, whole genome shotgun sequence.
ACCESSION   DADLSA010000006 DADLSA010000000
VERSION     DADLSA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.006"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            216..719
                     /locus_tag="JN691_000397"
     CDS             216..719
                     /locus_tag="JN691_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HAY7241928.1"
                     /translation="MGMIGYFADIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(786..2243)
                     /gene="nuoN"
                     /locus_tag="JN691_000398"
     CDS             complement(786..2243)
                     /gene="nuoN"
                     /locus_tag="JN691_000398"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAY7241929.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2250..3779)
                     /gene="nuoM"
                     /locus_tag="JN691_000399"
     CDS             complement(2250..3779)
                     /gene="nuoM"
                     /locus_tag="JN691_000399"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAY7241930.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3943..5784)
                     /gene="nuoL"
                     /locus_tag="JN691_000400"
     CDS             complement(3943..5784)
                     /gene="nuoL"
                     /locus_tag="JN691_000400"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAY7241931.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5781..6083)
                     /gene="nuoK"
                     /locus_tag="JN691_000401"
     CDS             complement(5781..6083)
                     /gene="nuoK"
                     /locus_tag="JN691_000401"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAY7241932.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6080..6634)
                     /gene="nuoJ"
                     /locus_tag="JN691_000402"
     CDS             complement(6080..6634)
                     /gene="nuoJ"
                     /locus_tag="JN691_000402"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAY7241933.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6646..7188)
                     /gene="nuoI"
                     /locus_tag="JN691_000403"
     CDS             complement(6646..7188)
                     /gene="nuoI"
                     /locus_tag="JN691_000403"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAY7241934.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7203..8180)
                     /gene="nuoH"
                     /locus_tag="JN691_000404"
     CDS             complement(7203..8180)
                     /gene="nuoH"
                     /locus_tag="JN691_000404"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAY7241935.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8177..10903)
                     /gene="nuoG"
                     /locus_tag="JN691_000405"
     CDS             complement(8177..10903)
                     /gene="nuoG"
                     /locus_tag="JN691_000405"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAY7241936.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10956..12293)
                     /gene="nuoF"
                     /locus_tag="JN691_000406"
     CDS             complement(10956..12293)
                     /gene="nuoF"
                     /locus_tag="JN691_000406"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAY7241937.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12290..12790)
                     /gene="nuoE"
                     /locus_tag="JN691_000407"
     CDS             complement(12290..12790)
                     /gene="nuoE"
                     /locus_tag="JN691_000407"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAY7241938.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12793..14595)
                     /gene="nuoC"
                     /locus_tag="JN691_000408"
     CDS             complement(12793..14595)
                     /gene="nuoC"
                     /locus_tag="JN691_000408"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAY7241939.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPTAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14689..15351)
                     /locus_tag="JN691_000409"
     CDS             complement(14689..15351)
                     /locus_tag="JN691_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAY7241940.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15367..15810)
                     /gene="nuoA"
                     /locus_tag="JN691_000410"
     CDS             complement(15367..15810)
                     /gene="nuoA"
                     /locus_tag="JN691_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAY7241941.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16441..17379)
                     /gene="lrhA"
                     /locus_tag="JN691_000411"
     CDS             complement(16441..17379)
                     /gene="lrhA"
                     /locus_tag="JN691_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAY7241942.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKSGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18299..19516
                     /locus_tag="JN691_000412"
     CDS             18299..19516
                     /locus_tag="JN691_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY7241943.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19600..20199
                     /gene="yfbR"
                     /locus_tag="JN691_000413"
     CDS             19600..20199
                     /gene="yfbR"
                     /locus_tag="JN691_000413"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY7241944.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20258..22090)
                     /locus_tag="JN691_000414"
     CDS             complement(20258..22090)
                     /locus_tag="JN691_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAY7241945.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASTAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22177..22827)
                     /gene="hxpA"
                     /locus_tag="JN691_000415"
     CDS             complement(22177..22827)
                     /gene="hxpA"
                     /locus_tag="JN691_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAY7241946.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(22838..23332)
                     /locus_tag="JN691_000416"
     CDS             complement(22838..23332)
                     /locus_tag="JN691_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAY7241947.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23415..23870)
                     /locus_tag="JN691_000417"
     CDS             complement(23415..23870)
                     /locus_tag="JN691_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAY7241948.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24208..25410
                     /gene="ackA"
                     /locus_tag="JN691_000418"
     CDS             24208..25410
                     /gene="ackA"
                     /locus_tag="JN691_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAY7241949.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25485..27629
                     /gene="pta"
                     /locus_tag="JN691_000419"
     CDS             25485..27629
                     /gene="pta"
                     /locus_tag="JN691_000419"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY7241950.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEAPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27819..29339
                     /gene="yfcC"
                     /locus_tag="JN691_000420"
     CDS             27819..29339
                     /gene="yfcC"
                     /locus_tag="JN691_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAY7241951.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29372..29914)
                     /gene="yfcD"
                     /locus_tag="JN691_000421"
     CDS             complement(29372..29914)
                     /gene="yfcD"
                     /locus_tag="JN691_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAY7241952.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29972..30522)
                     /gene="yfcE"
                     /locus_tag="JN691_000422"
                     /pseudo
     CDS             complement(29972..30522)
                     /gene="yfcE"
                     /locus_tag="JN691_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
     gene            complement(30578..31222)
                     /gene="yfcF"
                     /locus_tag="JN691_000423"
     CDS             complement(30578..31222)
                     /gene="yfcF"
                     /locus_tag="JN691_000423"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAY7241953.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31358..32005
                     /gene="yfcG"
                     /locus_tag="JN691_000424"
     CDS             31358..32005
                     /gene="yfcG"
                     /locus_tag="JN691_000424"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAY7241954.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKWLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32062..32424
                     /gene="folX"
                     /locus_tag="JN691_000425"
     CDS             32062..32424
                     /gene="folX"
                     /locus_tag="JN691_000425"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAY7241955.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32445..33338
                     /locus_tag="JN691_000426"
     CDS             32445..33338
                     /locus_tag="JN691_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAY7241956.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33386..34276)
                     /locus_tag="JN691_000427"
     CDS             complement(33386..34276)
                     /locus_tag="JN691_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY7241957.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34473..35246)
                     /gene="hisP"
                     /locus_tag="JN691_000428"
     CDS             complement(34473..35246)
                     /gene="hisP"
                     /locus_tag="JN691_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAY7241958.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35254..35970)
                     /locus_tag="JN691_000429"
     CDS             complement(35254..35970)
                     /locus_tag="JN691_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7241959.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILILHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(35967..36653)
                     /locus_tag="JN691_000430"
     CDS             complement(35967..36653)
                     /locus_tag="JN691_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708190.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY7241960.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(36743..37525)
                     /gene="hisJ"
                     /locus_tag="JN691_000431"
     CDS             complement(36743..37525)
                     /gene="hisJ"
                     /locus_tag="JN691_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAY7241961.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(37746..38528)
                     /gene="argT"
                     /locus_tag="JN691_000432"
                     /pseudo
     CDS             complement(37746..38528)
                     /gene="argT"
                     /locus_tag="JN691_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
     gene            complement(38794..39363)
                     /locus_tag="JN691_000433"
     CDS             complement(38794..39363)
                     /locus_tag="JN691_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY7241962.1"
                     /translation="MKRLIVGISGASGAIYGVRVLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39458..40975)
                     /gene="purF"
                     /locus_tag="JN691_000434"
     CDS             complement(39458..40975)
                     /gene="purF"
                     /locus_tag="JN691_000434"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAY7241963.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGVIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41012..41500)
                     /gene="cvpA"
                     /locus_tag="JN691_000435"
     CDS             complement(41012..41500)
                     /gene="cvpA"
                     /locus_tag="JN691_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAY7241964.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSYIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(41759..42421)
                     /gene="dedD"
                     /locus_tag="JN691_000436"
     CDS             complement(41759..42421)
                     /gene="dedD"
                     /locus_tag="JN691_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAY7241965.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPLAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42411..43679)
                     /gene="folC"
                     /locus_tag="JN691_000437"
     CDS             complement(42411..43679)
                     /gene="folC"
                     /locus_tag="JN691_000437"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAY7241966.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELLE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(43749..44663)
                     /gene="accD"
                     /locus_tag="JN691_000438"
     CDS             complement(43749..44663)
                     /gene="accD"
                     /locus_tag="JN691_000438"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAY7241967.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(44819..45478)
                     /locus_tag="JN691_000439"
     CDS             complement(44819..45478)
                     /locus_tag="JN691_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7241968.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(45561..46373)
                     /gene="truA"
                     /locus_tag="JN691_000440"
     CDS             complement(45561..46373)
                     /gene="truA"
                     /locus_tag="JN691_000440"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAY7241969.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46373..47386)
                     /locus_tag="JN691_000441"
     CDS             complement(46373..47386)
                     /locus_tag="JN691_000441"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY7241970.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47452..48588)
                     /gene="pdxB"
                     /locus_tag="JN691_000442"
     CDS             complement(47452..48588)
                     /gene="pdxB"
                     /locus_tag="JN691_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAY7241971.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQVRLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            48687..49682
                     /locus_tag="JN691_000443"
     CDS             48687..49682
                     /locus_tag="JN691_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAY7241972.1"
                     /translation="MIQPISGPPPRQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(49679..50857)
                     /locus_tag="JN691_000444"
     CDS             complement(49679..50857)
                     /locus_tag="JN691_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7241973.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQSLTGALTWGLGIVGLKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51132..52352)
                     /gene="fabB"
                     /locus_tag="JN691_000445"
     CDS             complement(51132..52352)
                     /gene="fabB"
                     /locus_tag="JN691_000445"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAY7241974.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            52511..54517
                     /gene="mnmC"
                     /locus_tag="JN691_000446"
     CDS             52511..54517
                     /gene="mnmC"
                     /locus_tag="JN691_000446"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAY7241975.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLFDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(54638..54916)
                     /locus_tag="JN691_000447"
     CDS             complement(54638..54916)
                     /locus_tag="JN691_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAY7241976.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(54950..55498)
                     /locus_tag="JN691_000448"
     CDS             complement(54950..55498)
                     /locus_tag="JN691_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAY7241977.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55498..56307)
                     /locus_tag="JN691_000449"
     CDS             complement(55498..56307)
                     /locus_tag="JN691_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAY7241978.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEYRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56307..57131)
                     /gene="mepA"
                     /locus_tag="JN691_000450"
     CDS             complement(56307..57131)
                     /gene="mepA"
                     /locus_tag="JN691_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAY7241979.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57135..58220)
                     /gene="aroC"
                     /locus_tag="JN691_000451"
     CDS             complement(57135..58220)
                     /gene="aroC"
                     /locus_tag="JN691_000451"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAY7241980.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58255..59187)
                     /gene="prmB"
                     /locus_tag="JN691_000452"
     CDS             complement(58255..59187)
                     /gene="prmB"
                     /locus_tag="JN691_000452"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY7241981.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNQWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTCRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            59353..59904
                     /gene="smrB"
                     /locus_tag="JN691_000453"
     CDS             59353..59904
                     /gene="smrB"
                     /locus_tag="JN691_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAY7241982.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60008..60898)
                     /locus_tag="JN691_000454"
     CDS             complement(60008..60898)
                     /locus_tag="JN691_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAY7241983.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSVAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(60885..61409)
                     /locus_tag="JN691_000455"
     CDS             complement(60885..61409)
                     /locus_tag="JN691_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7241984.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALSGFL
                     AFDSSSSASGAGIGIETAAGTSVPVNNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61406..61876)
                     /locus_tag="JN691_000456"
     CDS             complement(61406..61876)
                     /locus_tag="JN691_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7241985.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(61873..62421)
                     /locus_tag="JN691_000457"
     CDS             complement(61873..62421)
                     /locus_tag="JN691_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7241986.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
BASE COUNT        15546 a        17017 c        15625 g        14234 t
ORIGIN      
        1 tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg aattatcaga
       61 aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa taataaaaat
      121 ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg tctgttttaa
      181 gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc tattttgcag
      241 acattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct ttgatggata
      301 atatacatga cacgctttca ggattacgcc gactggatat tgataaacgc tgggattttt
      361 tacactttgg gctgacaggc acctctgctt ttgatcccgc gaagaatgat ccgctgagtc
      421 gtgccgtgct tgatgaacac agcctcgaag atggcataga tggcttcctg ggattgacct
      481 ggaatcagga attagccgcg acaatcgacc gactggaatc actggatcga agtgagctgc
      541 gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga gtgacctttt
      601 cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa aagctgatta
      661 gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt gttgggtgat
      721 ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac ttacaaacta
      781 acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg tggccataca
      841 cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc gctgtactgc
      901 cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag gctcaccgcc
      961 acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc caccagccac
     1021 cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa gcccagcgtc
     1081 atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag gatcggacga
     1141 tgccagaaca aaccgcggta agagaacagg gaatcagcat ccgggccacg atacgggctg
     1201 gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc ggccaggtaa
     1261 acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc caccagcaga
     1321 tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt ctggctcaac
     1381 gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac tacgcgaatc
     1441 gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc gaagatagcg
     1501 attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg gtatacgtct
     1561 ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac aatcatcatg
     1621 ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt tttacccagc
     1681 gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa cagcaggaaa
     1741 gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga acgtttctgg
     1801 cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat accgaggaac
     1861 agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc aattaacacc
     1921 aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta ggcgaaagta
     1981 caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa accatcaacg
     2041 cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag cgaaaccagc
     2101 gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg tcgccacgca
     2161 atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag cagtgcgatc
     2221 aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac ggaattaaca
     2281 aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg ctgcggatag
     2341 aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc acgcagcgac
     2401 atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta agcgcgatgc
     2461 aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat aacggtaatt
     2521 accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa gttaccggtg
     2581 ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag ccatttcatc
     2641 ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc ataaagctga
     2701 ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg gattaccgcg
     2761 ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc catgtgggaa
     2821 accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat ccacgcaccg
     2881 tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc cgacgcgttc
     2941 gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag caagatcccc
     3001 gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag ccagccatgc
     3061 agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat caacagatat
     3121 tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt ccagacgccg
     3181 gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac cagaccactc
     3241 gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc gtcagaggct
     3301 ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc ccagaagaag
     3361 aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag gatccacatc
     3421 aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca tagtaccgcc
     3481 agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc aatggcgaga
     3541 tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg ccactgcgga
     3601 attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag cgacagcgcc
     3661 agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc aaagcgttcg
     3721 gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg tagtaacatg
     3781 gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa ttctcacgac
     3841 agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag cttatccgac
     3901 ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca gtaccatcaa
     3961 cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc gcagataacc
     4021 gttctcgctt aacagcagac ctttacctgc aaagcgggaa agaaccgccg ggatgttcat
     4081 cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg gcttgacgaa
     4141 cactttgtcg tacagccagt caaagcccca ggcgttgtac caccaggtac tcagcaggcg
     4201 gcccggcgca ctgttggcga tggaggtcac cagagtacgt ttacccagcc acagccaggc
     4261 tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg tcaacatgct
     4321 gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta caatcagtgc
     4381 gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag ttaccccttt
     4441 cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca tacggaaggt
     4501 gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga tatgaccatt
     4561 cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga ccagcggcag
     4621 tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa tagatttacg
     4681 cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga cggaaccgga
     4741 tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa tcgccgcatc
     4801 ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg tagagtaagc
     4861 gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca gcagcgtaac
     4921 cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca ggccgtgggt
     4981 acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca gcgcggagac
     5041 aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact gcgcagattt
     5101 accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat tgccgtcagc
     5161 aaagtgcgct ggtgccagtt ccaccatttc gcggaagttc agggtgccca gttcgttgta
     5221 aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga cgaacgcttt
     5281 cattgccgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca gataggagca
     5341 caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga gaaccagaac
     5401 taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc cctcttcacc
     5461 gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga ccaccgagag
     5521 catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt cgcctaccga
     5581 catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga aatcaacgcc
     5641 gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga tcgccgaaac
     5701 gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca atggcaaaat
     5761 aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat gttcaggttc
     5821 tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc tgccgccgcg
     5881 aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta gcttccggcg
     5941 accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag cataaacagc
     6001 agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc cgcgaggatc
     6061 agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg ctgtcgtctt
     6121 tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag gccacaacca
     6181 gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc ccgaacagcg
     6241 taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg ttaacaccga
     6301 ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg ccaatccaca
     6361 cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccaag ttgagcatca
     6421 tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt tccagcgcac
     6481 cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc gaaataatca
     6541 ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc gcaagtatgg
     6601 ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg gtaacaggct
     6661 cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc cgtcgattgc
     6721 catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg agatcagcag
     6781 atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga aatccggggt
     6841 taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa tgcagcgtga
     6901 gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg ctttttgcag
     6961 cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc aacgctcttc
     7021 gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca gatagaccgg
     7081 ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga tcatccagat
     7141 actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg tcttattgcc
     7201 ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat cagcgtcagc
     7261 ggcaggcaga ttttccagcc gaaggacatt acctggtcat aacgcggacg cggtaacgac
     7321 gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa ccagatgaat
     7381 ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa tgcagagatg
     7441 gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc ggaatattca
     7501 atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg gtgacggtga
     7561 cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg gatgacgttc
     7621 cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc ctgcgccacc
     7681 acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc agaagcacgc
     7741 atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag caccgcgtaa
     7801 accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc ggcaaccacc
     7861 caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa ggcaatcatc
     7921 ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc tttaaagaac
     7981 attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg gttaggtcca
     8041 taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa tgccccacag
     8101 gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat ctcaatcagt
     8161 tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc gcgccagcca
     8221 gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc gtcagtcctt
     8281 cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag acgcgtgtac
     8341 ctgcgttcac acccaacttc gcggcatccg ctgggttgag cttgatgtac ggctgcggca
     8401 tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac aggtggtagt
     8461 acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg ctggtgaagt
     8521 aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca aagcgcagtt
     8581 tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag cccggcgccc
     8641 aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc atcgagaagg
     8701 tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata ttggcgcgca
     8761 tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca cgaatacgga
     8821 atgtcgcatc cggcgcagca tctttgatac ccgccagttc cgggattttc gccacaacag
     8881 cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag gtgctgtgca
     8941 gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa taagccggat
     9001 cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta ccgtcgcttt
     9061 cagcaaagct ggcagcggaa agcaccagat gggcgttttc catgatcgct gtgcgttgat
     9121 gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg gtagcagaag
     9181 cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg gtttccagtt
     9241 cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg ctgttgacgg
     9301 aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct ttcgcgacgt
     9361 tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata atcaacggtt
     9421 tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc tcaggttcga
     9481 taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa cctaaacgcg
     9541 cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga cgagtgtcat
     9601 caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg attgccgcaa
     9661 tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct ttcaccgcct
     9721 gacgcactgc cagcgcgacg cgcgcgccgg tctgggtaac gtcttcgccc agcaccagta
     9781 ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct tcacgcagca
     9841 ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg tagaagtttt
     9901 cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc ggagaaccaa
     9961 taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt gcctgttcgg
    10021 cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga cgatccttca
    10081 ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg tttaccgtac
    10141 cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg ctgatgttac
    10201 agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat ttacggttgt
    10261 aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg accaggttac
    10321 cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg ttgtcgtgcg
    10381 cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc acacagcggt
    10441 agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc tgattacggt
    10501 gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg gtcatatcct
    10561 gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg ttggtcatta
    10621 accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg gaaataaagg
    10681 tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg tcttccgcgt
    10741 tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc gccggatgcc
    10801 agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg ttgtccgctc
    10861 cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg cttccagttt
    10921 tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt cagcaggttc
    10981 ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc cgcctcaaat
    11041 tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg tgcgtgggca
    11101 cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc gatatcgccc
    11161 ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg cagaccgtcg
    11221 cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag gttacgcacc
    11281 agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc cagacggctg
    11341 cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc ggtcaggaag
    11401 tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg cataccaccg
    11461 gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca cagtcccgga
    11521 tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact tttcgagatg
    11581 ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag ggtttcgacg
    11641 ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg gggtttcgag
    11701 cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc cccgcagatg
    11761 tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc cataatgttt
    11821 ttgccaagca agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt tactgccgct
    11881 tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc aaacgcggag
    11941 atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg gtctttatag
    12001 gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat ggattcgtct
    12061 ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg ccctttcaga
    12121 ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag cgccttacgc
    12181 gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg tttgtcatcg
    12241 cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt catttatacc
    12301 gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc tcatcgatca
    12361 tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc gtaaagcggc
    12421 catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc gcctgaatac
    12481 cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca tggcgaccaa
    12541 ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg cttgccggaa
    12601 tacccagcac atcggcgatc gcgtggatcg caccatcagg cacccagcca cgctgcttct
    12661 gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg tgcatctcgt
    12721 gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt ggttgttgat
    12781 tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc ccagataaac
    12841 aatcaggtca gacaccaggc tgccgcggat cgccgtcgga atttgctgca aatgcgcata
    12901 actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg tcaggtagta
    12961 actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag gcatcaccgg
    13021 cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca gcgtgcgctc
    13081 tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg gcatgttgtt
    13141 gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca gcattacgcg
    13201 ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt tttcatagcc
    13261 agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca ggcccgcgcc
    13321 agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct gggaacgacc
    13381 tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt tcggcatcca
    13441 gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg ctacgccgcc
    13501 aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca gatcgtaaat
    13561 tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt cctgaataaa
    13621 ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca taacgcgaat
    13681 gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca gcacgtaagg
    13741 catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa tgtagctgtg
    13801 ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga tgtctggtac
    13861 gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc cgtgcgccga
    13921 cgggtggttc ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc cgcgcttcat
    13981 cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt tggctttggt
    14041 cagctcaaac ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca gcgggtgacc
    14101 tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga aagtaatgcc
    14161 aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt tggtgaaggt
    14221 cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc ggttacgatc
    14281 gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt cgcggtgtgt
    14341 gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt tcggcagttt
    14401 ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa cgggaacccc
    14461 ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca gttcgccaat
    14521 taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg ttaagtcggt
    14581 catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag cttcgcgaaa
    14641 tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt aaatctcgtc
    14701 aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc gctctgattg
    14761 catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac gacgttcttt
    14821 gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac gcggcgggca
    14881 gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa cggaataaat
    14941 atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt ttggttccag
    15001 catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg ttcctgcaac
    15061 caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa aacgcgccac
    15121 gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa gaccgaagtt
    15181 atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct tgcccataaa
    15241 cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct tttgcagggg
    15301 gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca tcttaatgcc
    15361 tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac gctcgcggcg
    15421 tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca gacctgccag
    15481 taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt cgcggataga
    15541 ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga acatggccac
    15601 cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga taccggattc
    15661 aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac cgcctaccag
    15721 catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc agtgatgagc
    15781 gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag cgccagattc
    15841 actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa aaaaaacctt
    15901 acaatcactg tagaaattct tttatacagc taattgatgt ggtcttttac tcctttctat
    15961 aaccttttgt caactttaac aaaagtttct tcacattagt ttacataata tcaacaccat
    16021 tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt agatgttgtt
    16081 gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca ggaatttgtg
    16141 tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt tgttattcca
    16201 taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca ggtgattttt
    16261 ttcaggcgat tatttgtgcg ttcgggacgt gaatctctgg tgattgaaaa ataaacagtt
    16321 ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc tggggtttaa
    16381 aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat cgtccgtcga
    16441 ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag acattgggct
    16501 gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg ccagctcatt
    16561 attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg gtaagccatc
    16621 tactccactc agcacacgca ggtccgggct catcatctca accggccttg ccgtcacgcc
    16681 aagaccggat ttcactgccg cacgaacggc cggaagcgtc gaggcgacat aagccagtcg
    16741 ccatggaata tctgctttat taagcgtcgc cagcaccata tcgcgaaacg gacttggatc
    16801 atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg ctgcgcagta
    16861 ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac gatgggtggt
    16921 taccatcaaa tccacttcct gggattccag catttctgcc atataggcat tacgtttaac
    16981 gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca ggaaaggtaa
    17041 gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa gattactgaa
    17101 cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt aaccaagaag
    17161 ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca gttctttccc
    17221 aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac gacacacagc
    17281 ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc tcagcagatc
    17341 gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact tactggcggc
    17401 tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt gtaatgtgcc
    17461 tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag ttgcactaaa
    17521 aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata tatcttcagg
    17581 gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg tgtttgtata
    17641 aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata cgcgaacacg
    17701 tacaaacagc aatggtgaag cggcatcaag agcagggatc cgtcatttat gcgagcattt
    17761 taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca aagttatgtt
    17821 atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt tgaatattaa
    17881 ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag tttcacacct
    17941 tacccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta atgcggaaat
    18001 atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca attatcttac
    18061 agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata acattgcatg
    18121 taaagcatat acacctcatt attttgtcat tattaagtta ttaacagcac aatcgagcct
    18181 tcccctctgg caaaatctta ttctgcagac cttcaaaaca ccgtcctggg ggagtacatt
    18241 gttctaagct gacttccacg gcagggagtg gcgataacag caaaaaaggt caagattcat
    18301 gtcccccatt gaaaaatcca gcaaattaga gaatgtctgt tatgacatcc gtggtccggt
    18361 gctgaaagaa gcaaaacgcc tggaagaaga aggtaacaag gtactgaaac tgaacatcgg
    18421 caacccagcc ccattcggtt ttgacgcgcc agatgaaatc ctcgttgacg tgatacgcaa
    18481 cctgcctacc gctcaagggt attgcgattc caaagggctt tactccgcgc gtaaagccat
    18541 catgcagcac taccaggctc gtggcatgcg tgatgttacc gtggaagata tttacatcgg
    18601 caatggtgta tcggagctta tcgttcaggc aatgcaggca ttgctgaaca gcggggacga
    18661 aatgttggtt cctgcaccag attacccact ctggaccgcg gcggtttcgc tttccagcgg
    18721 taaagcggtg cattatcttt gcgatgaatc ctctgactgg ttcccggacc tcgatgatat
    18781 tcgcgctaaa attacgcctc gtacgcgtgg gattgttatt atcaacccaa ataacccaac
    18841 cggcgcggta tattccaaag agcttttaat ggagattgtg gagattgcac gtcagcataa
    18901 tctcattatc ttcgccgatg aaatttatga caaaattctc tacgacgatg ctgagcatca
    18961 ctcaattgcg ccgctggcac ctgacctgct gaccattacc tttaacggac tgtcgaaaac
    19021 gtaccgcgtt gcaggcttcc gtcaggggtg gatggtgttg aacgggccga aaaaacacgc
    19081 caaaggctac atcgaaggtc tggaaatgct ggcttcaatg cgcctgtgtg ctaacgttcc
    19141 tgcgcaacac gccattcaga ccgcgctggg tggttatcag agcatcagtg aatttattac
    19201 ccctggcggt cgtctttatg agcagcgtaa ccgcgcgtgg gaactgatca acgatattcc
    19261 gggcgtttcc tgcgtgaaac ctcgtggtgc gctgtatatg ttcccgaaaa tcgacgccaa
    19321 acgctttaac attcatgacg atcagaaaat ggtgctggat ttcctgttgc aggaaaaagt
    19381 tctgttggtg caagggacgg cattcaactg gccgtggccg gatcacttcc gcattgtcac
    19441 gctaccgcgt gtcgatgata tcgagctgtc tttgagcaag ttcgcgcgtt tcctttctgg
    19501 ttatcatcag ctgtaatctt aatttcactg ccggagattg catccggcag tgttatcccg
    19561 ccacaatgac ctgatgatgt catcatacgt aaggtcacta tgaaacagag ccattttttt
    19621 gcccatctct cccgcctgaa actcattaac cgctggccgc taatgcgcaa cgtgcggacg
    19681 gaaaatgtgt ccgaacacag tttgcaggta gcgatggtcg cccatgcgct ggcagctatc
    19741 aaaaatcgga aatttggcgg taatgtcaac gccgaacgta tcgctttact ggcgatgtac
    19801 cacgatgcct cagaagtgct caccggcgat ctccctactc cggtgaaata cttcaattcg
    19861 caaatcgctc aggagtacaa ggctattgaa aaaatcgctc agcagaaact ggtcgatatg
    19921 gttccggaag aactgcggga tatctttgcg ccgttaattg acgagcatgc atatagcgat
    19981 gaagaaaaat cgctggtgaa acaggcagat gcactgtgtg catatctgaa atgtctggaa
    20041 gaactcgcgg ccggaaataa tgaattcttg ctggcaaaaa cgcgactgga agcgacgctt
    20101 gaagcgcgtc gcagccagga gatggactac ttcatggaag tatttgttcc cagcttccat
    20161 ctttcgctcg atgagattag ccaggattca ccgctgtaag cagccggagt ctgcgtcgca
    20221 tcaggcaata agcgccggat gcgacatcag gctcttgtca aaacggaaac agcatcggga
    20281 tcatcaccac acaaaccgcc atcacgataa tggtgaacgg tacccccaac ttcacaaagt
    20341 cactgaagct gtaatttccc ggacctaaaa ccagggtgtt aacaggtgaa gacaccggtg
    20401 tcataaaggc ggcggatgct gccatcgcca cgaccatcgc aaatggataa ggcgacactc
    20461 ccatcgtttt ggcagcagcc agcgcaatcg gagccatcaa caccgccgtc gcggtattag
    20521 agataaacag cccaataacc gccgacaaga caaacaaaca ccccagcatc atatgtggcc
    20581 cataaccgcc gccaatatcc atcagccctt tcaccgccag cgcgacacct cccgttttct
    20641 gtaatgccac agcaaacggc atcatcccaa cgatcaaaat aatgctcggc cagtgaatgg
    20701 atttataggc gctttcagca tctatacagc ggaatttccc catcagcagg caggcgatga
    20761 tagcggcaac aggattagga atttcatctg tcagcattaa cgccaccatt agcaccagac
    20821 agaaaatggc atggggtgcc tggctgtgcg cggtcgatgc ttcactcacc tcttccggta
    20881 agttcagcgc tacgaagtcg cggccctgtt tggccagcat accgatcagt ttccagttac
    20941 ccacaaccag gatgatatcg cccagcagca gaggctcatc cgccagcgaa ccttccagcg
    21001 ccacgccatt gcgcttgagc cccaccacat tcagtccgta gcgggtacga aaaccaattt
    21061 cgcgcaccga tttaccaatc agttctgact caggaattaa tgaaatctct gccatgccca
    21121 catcaagggc ctggtcagaa aaatactcgc cgcgcagtac catcggctcc agcaattgct
    21181 cactacaaaa ttgccggaga tcgacatcag ccgcagacat atcaataagc aaaacgtcac
    21241 gcgcgcgaaa ttcagaaacc ccattaacgt tcacgataac gcgacgaaaa cgccgccagc
    21301 gttcaacacc gatgacgtta gcgccataac gctcacgtaa tttgagatca tccagccgtt
    21361 gacctatcat tggcgatccg gggcgaatag ccagacgtcg cgcacgcccg gtcagtcgat
    21421 attcacggat aagatcgcga aaggttcgac gcgtccagcc ttcgcgctgc ggggtctggg
    21481 tatccccttt cagcatgaaa cgcatcacta acatatacaa aatacccagc accagcacga
    21541 ccaggccaat aggtgttacg ctaaagaaac tgaagccgtg atagccttca cgcagcaatt
    21601 cactgttgac taccaggttc ggcggcgtcg ccaccagcgt catcatgccg ctaatcagcc
    21661 cggcaaaact taacggcatc atcagacgtg acggcgaagt ttgcatacgc atggcaacgc
    21721 ttaacaccac gggaataaag atagcgacaa cgccggttga actcataaac gcgccaagcc
    21781 ccgcgacggt cagcatcaac aaaaccaaca ttttgatttc actgttgccc gccactttga
    21841 ccagccatgt tcccattacg gtggcaacac cggtacggac caaaccatcg ccaataataa
    21901 acaaggcggc aatcaggaca acgttaggat cagaaaagcc ggaaaatact tcggggaccg
    21961 tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa agcgaccgca tccatacgca
    22021 ctctgcccgt cgcaaacaag acgatggcaa cggccagtaa tgaaagaacc caaatcaatt
    22081 caccgttcac aacttatcct tgttaattga gggggatgac ttgattctgc cataaaaaag
    22141 ccccgacgag acgaggctaa atcatgatca ggtgtttcac tgaataataa catcgccatt
    22201 tggctgtttg gtcacagtaa tttgctccag actgtggagg accaaatcga cctcattcag
    22261 gcgcggggta tctgccggag cgttaaccgc aatgacatgg caacccgccg ccaggccaga
    22321 aagcacgcca gcgggagcat cttccaccac cacacactcc tgcggcgcaa gccccagcag
    22381 ctgcgcacct aacagatacg catcaggttc tggttttccg cgcttcactc gctcagcggt
    22441 tacaaacacc tctggtgcgg gaagccccgc tattttatgg cgcgctcgcg ctaccggcat
    22501 ggagccagaa gtcacaatgg cccacggaat acctgcttta ttcaaatgat tgagtaaggc
    22561 gattgcccca ggaagcgcgg taataccttc ggtttccgtg gcctcgattt gctccagacg
    22621 cgtaaactcg gcggcaatat cagcctcgga tttgcccgcc ataaaatggc gcaaagaggt
    22681 gatcgcctgt ttaccgtgaa tgaaagccag cacctcttcc ggcgctaacc catgacgtct
    22741 ggcccagttg ctccacgccc gttctaccgc aggcagggaa tccaccagcg ttccatcaag
    22801 atcaaacaga aaacctttgc accgcacgcg ggcctcctca ggcgttgata atttgattaa
    22861 tttcgttggc gctcaaatgg tactgacgcg ggcaggcatg ccacacatta agcatgcgct
    22921 gatatttttc ccacattggc gtctgggcgt taaaaccatg agtcccagcg tcaaaatggg
    22981 tatagcgccc ttccacatta accataaagc ggacataacc gaggtaacgt gcttcagtgg
    23041 cggcgtcaaa gccgaggaag gtgacacgac gttcatcgat ggattgctgg tcctgcaaat
    23101 tagaccagga aacatgcaac gcatgataca tctccataat gtcgatgata gtgcggcagg
    23161 tttcttcttt cagctcgcca aactcgcgat ccaattcacg catctgtaat ccgtaaccac
    23221 gctcaataat tgtttgcagg cgacggtaac gttcagcatt tgccggatcg agcatagtca
    23281 tcatcttgta ctgattagac aaaataagac gttgcgcgtt ggtcatttcc attgttgact
    23341 cctgtatcac tctactacgg tgaaaaaaaa gaaggctgag tatgccttct tttatatgcg
    23401 taatcagggg tcaattacaa atcatcaagg aaagttttat ccagttgttt gaaggcgcgc
    23461 ttaagcgtgt cagctaatgc ctggtaatca ggcttgcctt caacgggtgc caacacctgt
    23521 ccagactcct gcaatttacc gcgaacttca taaaaccagt taaggattgc agggggtaat
    23581 ggcgttacag aacgcttgcc cagccaccac aatccctgca tgggtaaact taaggcgaac
    23641 agtgcagtgg caactgccgg cccaagcaga ccgcccaggg caatctgcca gcagagagta
    23701 aatacggcga tcggcggcat aaaacggatc gcataacgcg tcatcttgat aacgcgattt
    23761 tcgacaaaga ccggggcaag gcgtttttcc agtggccacg tctttgagta atgctgtccg
    23821 cggcgaaaca agctaaaaaa attaacagaa cgattatccg gcgttgacat gcttcacctc
    23881 aacttcacat ataaagattc aaaaatttgt gcaaattcac aactcagcgg gacaacgttc
    23941 aaaacatttt gtcttccata cccactatca ggtatccttt agcagcctga aggcctaagt
    24001 agtacatatt cattgagtcg tcaaattcat atacattatg ccattggctg aaaattacgc
    24061 aaaatggcat agactcaaga tatttcttcc atcatgcaaa aaaaatttgc agtgcatgat
    24121 gttaatcata aatgtcggtg tcatcatgcg ctacgctcta tggctccctg acgttttttt
    24181 agccacgtat caattatagg tacttccatg tcgagtaagt tagtactggt tctgaactgc
    24241 ggtagttctt cactgaaatt tgccatcatc gatgcagtaa atggtgaaga gtacctttct
    24301 ggtttagccg aatgtttcca cctgcccgaa gcacgtatca aatggaaaat ggacggcaat
    24361 aaacaggaag cggctttagg tgcaggcgcc gctcacagcg aagcgctcaa ctttatcgtt
    24421 aatactattc tggcacaaaa accagaactg tctgcgcagc tgactgctat cggtcaccgt
    24481 atcgtacacg gcggcgaaaa gtataccagc tccgtagtga tcgatgagtc tgttattcag
    24541 ggtatcaaag atgcagcttc ttttgcaccg ctgcacaacc cggctcacct gatcggtatc
    24601 gaagaagctc tgaaatcttt cccacagctg aaagacaaaa acgttgctgt atttgacacc
    24661 gcgttccacc agactatgcc ggaagagtct tacctctacg ccctgccgta caacctgtac
    24721 aaagagcacg gcatccgtcg ttacggcgcg cacggcacca gccacttcta tgtaacccag
    24781 gaagcggcaa aaatgctgaa caaaccggta gaagaactga acatcatcac ctgccacctg
    24841 ggcaacggtg gttccgtttc tgctatccgc aacggtaaat gcgttgacac ctctatgggt
    24901 ctgaccccgc tggaaggtct ggtcatgggt acccgttctg gtgatatcga tccggcgatc
    24961 atcttccacc tgcacgacac cctgggcatg agcgttgacg caatcaacaa actgctgacc
    25021 aaagagtctg gcctgctggg tctgaccgaa gtgaccagcg actgccgcta tgttgaagac
    25081 aactacgcga cgaaagaaga cgcgaagcgc gcaatggacg tttactgcca ccgcctggcg
    25141 aaatacatcg gtgcctacac tgcgctgatg gatggtcgtc tggacgctgt tgtattcact
    25201 ggtggtatcg gtgaaaatgc cgcgatggtt cgtgaactgt ctctgggcaa actgggcgtg
    25261 ctgggctttg aagttgatca tgaacgcaac ctggctgcac gtttcggcaa atctggtttc
    25321 atcaacaaag aaggtacccg tcctgcggtg gttatcccaa ccaacgaaga actggttatc
    25381 gcgcaagacg cgagccgcct gactgcctga tttcacaccg ccagctcagc tggcggtgct
    25441 gttttgtaac ccgccaaatc ggcggtaacg aaagaggata aaccgtgtcc cgtattatta
    25501 tgctgatccc taccggaacc agcgtcggtc tgaccagcgt cagccttggc gtgatccgtg
    25561 caatggaacg caaaggcgtt cgtctgagcg ttttcaaacc tatcgctcag ccgcgtaccg
    25621 gtggcgatgc gcccgatcag actacgacta tcgtgcgtgc gaactcttcc accacgacgg
    25681 ccgctgaacc gctgaaaatg agctacgttg aaggtctgct ttccagcaat cagaaagatg
    25741 tgctgatgga agagatcgtc gcaaactacc acgctaacac caaagacgct gaagtcgttc
    25801 tggttgaagg tctggtcccg acacgtaagc accagtttgc ccagtctctg aactacgaaa
    25861 tcgctaaaac gctgaatgcg gaaatcgtct tcgttatgtc tcagggcact gacaccccgg
    25921 aacagctgaa agagcgtatc gaactgaccc gcaacagctt cggtggtgcc aaaaacacca
    25981 acatcaccgg cgttatcgtt aacaaactga acgcaccggt tgatgaacag ggtcgtactc
    26041 gcccggatct gtccgagatt ttcgacgact cttccaaagc taaagtaaac aatgttgatc
    26101 cggcgaagct gcaagaatcc agcccgctgc cggttctcgg cgctgtgccg tggagctttg
    26161 acctgatcgc gactcgtgcg atcgatatgg ctcgccacct gaatgcgacc atcatcaacg
    26221 aaggcgacat caatactcgc cgcgttaaat ccgtcacttt ctgcgcacgc agcattccgc
    26281 acatgctgga gcacttccgt gccggttctc tgctggtgac ttccgcagac cgtcctgacg
    26341 tgctggtggc cgcttgcctg gcagccatga acggcgtaga aatcggtgcc ctgctgctga
    26401 ctggcggcta cgaaatggac gcgcgcattt ctaaactgtg cgaacgtgct ttcgctaccg
    26461 gcctgccggt atttatggtg aacaccaaca cctggcagac ctctctgagc ctgcagagct
    26521 tcaacctgga agctccggtt gacgatcacg aacgtatcga gaaagttcag gaatacgttg
    26581 ctaactacat caacgctgac tggatcgaat ctctgactgc cacttctgag cgcagccgtc
    26641 gtctgtctcc gccagcgttc cgttatcagc tgactgaact ggcgcgcaaa gcgggcaaac
    26701 gtatcgttct gccggaaggt gacgaaccgc gtaccgttaa agcagccgct atctgtgctg
    26761 aacgtggtat cgcaacttgc gtactgctgg gtaatccggc agagatcaac cgtgttgcag
    26821 cgtctcaggg tgtagaacta ggtgcaggca ttgaaatcgt tgatccagaa gtggttcgcg
    26881 aaagctatgt tggtcgtctg gtcgagctgc gtaagaacaa aggcatgacc gaaaccgttg
    26941 cccgcgaaca gctggaagac aacgtggtgc tcggtacgct gatgctggaa caggatgaag
    27001 ttgatggtct ggtttccggt gctgttcaca ctaccgcaaa caccatccgt ccgccgctgc
    27061 agctgatcaa aactgcaccg ggcagctccc tggtatcttc cgtgttcttc atgctgctgc
    27121 cggaacaggt ttacgtttac ggtgactgtg cgatcaaccc ggatccgacc gctgaacagc
    27181 tggcagaaat cgcgattcag tccgctgatt ccgctgcggc cttcggtatc gaaccgcgcg
    27241 ttgctatgct ctcctactcc accggtactt ctggtgcagg tagcgacgta gaaaaagttc
    27301 gcgaagcaac tcgtctggcg caggaaaaac gtcctgacct gatgatcgac ggtccgctgc
    27361 agtacgacgc tgcggtaatg gctgacgttg cgaaatccaa agcgccgaac tctccggttg
    27421 caggtcgcgc taccgtgttc atcttcccgg atctgaacac cggtaacacc acctacaaag
    27481 cggtacagcg ttctgccgac ctgatctcca tcgggccgat gctgcagggt atgcgcaaac
    27541 cggttaacga cctgtcccgt ggcgcactgg ttgacgatat cgtctacacc atcgcgctga
    27601 ctgcgattca gtctgcacag cagcagtaat ctcgtcatca tccacagctt tgcgctgcgg
    27661 atatctgaac cggaaataat cactatttcc ggttttttat tctcttaatt tgcattaatc
    27721 ctttctgatt atcttgctta actgcgctgc atcaatgaat tgcgccatct cactttgcat
    27781 acttaccact ttgttttgtg caagggaata tttgcgctat gtccgcaatc actgaatcca
    27841 aaccaacaag aagatgggca atgcccgata cgttggtgat tatctttttt gttgctattt
    27901 taaccagtct tgccacctgg gtagttccgg tggggatgtt tgacagtcag gaagtgcagt
    27961 atcaggttga tggtcaaaca aaaacacgca aagtcgtaga tccacactca tttcgcattc
    28021 tgactaacga agcaggcgaa cctgagtatc accgcgtaca gctgttcacg acgggcgatg
    28081 aacgcccggg cctgatgaac ttcccgtttg aagggttaac ctcaggatcg aaatacggga
    28141 cagccgttgg catcatcatg tttatgctgg tgattggcgg cgcgtttggc attgtgatgc
    28201 gtacaggaac cattgataac ggtatcctgg cgcttattcg ccatacccgc gggaatgaaa
    28261 ttctctttat tcctgcgctg tttattctgt tttcacttgg cggcgcggta tttggtatgg
    28321 gagaagaggc cgtcgccttt gccattatca tcgcaccgct aatggtccgg ctgggctatg
    28381 acagtattac caccgtcctg gtgacttata ttgccacgca aatcggtttt gccagttcgt
    28441 ggatgaaccc gttttgtgtg gttgttgctc aggggattgc cggcgttccg gtgctttctg
    28501 gctccgggtt gcgcatcgtg gtgtgggtta tcgccactct gattggcctg atctttacca
    28561 tggtgtacgc ctcacgagtg aaaaagaatc ctcttctgtc acgcgtgcat gagtccgacc
    28621 gcttctttcg tgaaaagcag gcggatgttg aacaacgtcc gtttaccttt ggtgactggc
    28681 tggtattgat tgtcctgaca gccgtaatgg tctgggtgat ttggggcgtg atcgttaatg
    28741 cctggtttat tccagaaatt gccagccagt tcttcaccat gggtctggtg attggcatca
    28801 tcggcgttgt tttccgcctt aacggcatga cggttaatac catggcttca tcctttaccg
    28861 aaggggcgcg aatgatgatc gcccctgccc tgctggtggg tttcgccaaa gggattttgc
    28921 tgctggttgg taatggtgaa gcaggtaatg ccagcgtgtt aaatactatc ctcaacagca
    28981 ttgccaatgc cattagcggt ctggacaacg cggtcgcggc ctggtttatg ttgctcttcc
    29041 aggctgtatt taatttcttc gtgacgtccg gttctggtca ggcggcgtta actatgccgt
    29101 tactggcacc gcttggcgat ctggtcggtg ttaaccgtca ggttaccgtg ctggcgttcc
    29161 agtttggtga tggcttcagc cacatcattt acccaacctc agcttcgtta atggcaacgc
    29221 tcggtgtgtg ccgggtggac ttccgtaact ggctgaaggt gggcgcgacc ctgcttggac
    29281 tgctgtttat tatgtccagc gtcgtagtga tcggcgctca gttgatgggc taccactaaa
    29341 aatgttaaga gccgcaattg cggctctttt ttcattctgc cgtttcagtc tctacggctt
    29401 catttttggc attgcgcttc atccacaacg ccagcgcttt cagcgagtct ggagtgaact
    29461 catcgcagcg tgcggtgatt tcttccggcg tcagccagca aacttcactg acttcatctt
    29521 cctgtagggc gaagggaccg tgagagacgc aactgaacaa cgcgccccag acacggcaat
    29581 ttttatcttc gaaatagaac tgcccgtgct cggcaaaggg gacaccggca atgcccaact
    29641 cttcttccgc ttcgcgacgc gcggattcca gcagttgctc atcggcctga actacaccgc
    29701 ctgcggtcgc atctaacatg ccgggtaaaa agtcttttgt ctcggtacga cgctggacca
    29761 gaattttgcc catgccatca tgcacgacga tgtaagttgc acgatgacgc agacactgtg
    29821 cccgcatttg ttcccggctg gcttgtgcaa tgacttcgtt ctcttcattg acaatatcca
    29881 cccattcagt acttgccaaa cgacgctgtt ccattatcag gaaaccttct ttttctggcg
    29941 ctcttacggc gcattttgag ttgtgggtaa attacggatt aatcgcgacc tgcgcaatga
    30001 tactttgatc attgagtgcg attacactaa gtacgtcatt atccagcatg ccataactcg
    30061 ccggattacc gcctttcgga atactcaccg agccggggtt gaagtggaaa atctcccccc
    30121 gctgttctgc cactggcaaa tgggtatgac cgtacaccag cacatcgttc tggtttaaag
    30181 caggtagatt ttccgggcca aaaagatgac catgcgtcaa aaacagacgt tgtttttcca
    30241 gtaatacctg ttgccacggc gcggttatcg ggaaatgcag catcatttga tccacttcgc
    30301 tgtcgcagtt gccacgcaca gcgataacct tatgtgccac ttcattaagc cgttcagcga
    30361 ctttggctgg cgcgtaaccc tccggtaaag cattacgcgg ccgtgattca acacgtcgcc
    30421 aaggatcacc agccactggg caccgctttg gacaaacaac tccacaacac gttccgtcgc
    30481 cggtaacgac ccatgaatgt ccgatgcaaa catcagtttc atcactcact cctcgtcgaa
    30541 aaaacgtaag ctatgatact ggattctgtc gttgctatca gccagattgc ttcgccgaaa
    30601 gtgcaataaa acgctggaca gacgctcgct gccactggaa tgtcgcataa tccaccaggc
    30661 gttctggcac ctcatcgcca tgtagtacca ggcggttaat cattagcgcc agatcagtat
    30721 cagcaatgca ccattcacca aataaattcg gctgacccag tgctaacaaa tgttctgcca
    30781 tcgcgaacag tttctctgca ctggcttttc cctcggccgt tagtggcgct tttttcgccc
    30841 ccgcaaagac aacatccgtc ggacgctctt cgcggatggg catcagatcg ctgcgcagcc
    30901 aggcctgaat ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa atacgttccc
    30961 aggtcggtgg cgcaaatcga tcttccagat actccgcaat ggcagaagat tcactcaact
    31021 caaaatcatc gatttgtaat aacggcacac ggcgtgtctg accgtaacct tgccacgtcg
    31081 gctgcaaatg ttcaccgctg tcgaggtcga tggtcttgat atgaaacgac aggccttttt
    31141 cctgcaacgc cacccaggcg gataacacat aaggggagaa aaagtgggca tctgaccaaa
    31201 gcgtgatagc gggtttactc attatgtcct cggcaataaa gggggctggc tatcaaacta
    31261 tagagccttt ttgtcactgt cacgtgacga atactcacgt aagtcgccgc gctttctata
    31321 cttgtggtgt tcagatgatt aaacaaccgg agctgcaatg atcgatctct atttcgcccc
    31381 gacacccaat ggtcacaaaa ttacgctgtt tctcgaagaa gcagagcttg attatcgctt
    31441 gattaaggta gacctgggga aaggtggtca gtttcgtccg gaatttttgc gcatttcgcc
    31501 caacaacaaa attccggcaa ttgttgatca ttctcctgcc gatggcggcg aaccgttaag
    31561 cctctttgag tctggtgcca ttttgttgta tctggctgag aaaacaggac tctttttgag
    31621 tcatgaaacg cgtgagcgcg ccgccacatt acagtggtta ttctggcagg taggcggact
    31681 ggggccgatg cttgggcaaa atcatcattt taatcacgca gccccccaaa ccattcctta
    31741 cgctattgaa cgttatcagg ttgaaactca gcgtctttac catgtactaa acaagtggct
    31801 ggaaaactcg ccctggctgg gaggcgagaa ctacagcatt gcggatattg cctgctggcc
    31861 gtgggttaat gcctggactc gcctgcgaat tgacctcgca atgtatccgg cagtcaagaa
    31921 ctggcatgag cggatccgtt cgcgccctgc caccgggcag gcactgctaa aagcacaact
    31981 cggtgatgag cgttcggata gttaacagaa acaggttctc gtgtattatt tcatcctaag
    32041 taaaacaacg gagaacctgc aatggcacaa cctgccgcta ttattcgtat aaagaacctt
    32101 cgtttgcgta cgtttatcgg aattaaggaa gaagaaatta acaaccgtca ggatattgtt
    32161 atcaatgtga cgatccacta ccccgccgat aaagcgcgca ctagcgaaga tatcaacgat
    32221 gcgctgaatt atcgcaccgt aacgaaaaac attattcagc atgtagagaa taaccgtttc
    32281 tctttgctgg aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca tcactgggtg
    32341 acgtatgctg aagtggagat cgataaactg cacgcgctgc gctacgccga ttcggtatcc
    32401 atgaccttaa gctggcagcg ttaatcgcca tatcgggagg ctgcatgaac atagtgatca
    32461 ccggagggac gggattaatt ggtcgccatt tgattccgcg tttgctggag ctgggccatc
    32521 aaattacggt agtgacgcgt aacccgcaga aagccagttc cgttctcggc cctcgggtga
    32581 cactatggca agggcttgcc gatcaaagca acctcaacgg cgttgatgcg gtaatcaacc
    32641 tggccggaga accgattgct gataaacgct ggactcacaa gcaaaaagag cgtctctgcc
    32701 aaagccgctg gaatatcacg caaaaactgg tcgatttgat taatgccagc gacacgccgc
    32761 cgtcggtact catttccggc tcggcaacgg gctattatgg cgacttaggt gaagtggtgg
    32821 ttaccgaaga ggaaccgccg cataacgaat ttactcataa actctgcgcc cgctgggaag
    32881 agattgcctg ccgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcga accggcgtgg
    32941 tactggcacc ggacggcggt attctcggta aaatgctgcc accgtttcgt cttggcctgg
    33001 gcgggccgat tggttccggt cggcagtatc tggcctggat tcatattgat gatatggtca
    33061 acggcattct ctggctgctg gataacgagc tgcgcgggcc atttaatatg gtttcgccct
    33121 acccggtacg caatgaacaa tttgcccatg cgctcggtca tgcgctgcat cgcccggcta
    33181 ttttgcgcgt ccccacaacc gccattcggc tgttaatggg cgaatcttca gtactggtat
    33241 taggtggaca acgcgcgctg cctaaacggc tggaagaagc gggttttgcg tttcgctggt
    33301 acgatttaga agaggcgctg gcggatgtcg ttcgctgatg tggtttacag caaacatccg
    33361 ccagttaact ccctgtgtta caggattagt ggctttgcgc gataagatcg tctggtgaaa
    33421 gtcgggtcac catcataact aactctctgt ctaaacctct atccagcatc tcctgagcaa
    33481 tacgcagggc ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat ctgtcagcaa
    33541 tggtcatcag tttctccttt tcttgtggtg cgcgttctgt tatctcacca ataaatgcac
    33601 ggaaacgctg ggcatcccct gtttgtaata cgtaattaaa cagggctttt agctgtctgt
    33661 cattagtgtt ccctgtaact agcagcgaaa caatttggtc gactaatccc aacagatcgc
    33721 gctgacgaat atgtttctga attaactcca acagcgccat tttgcggtgt tgcataatct
    33781 cgtcatccgg caccacggta atatccacca acggaaacgc tgatgaatat attttgcggg
    33841 ctatagcagg ctcggcaaat tcatcaagcc agcagagtga ataaggataa ggacttctgc
    33901 aaccatgata aaacagcatc gggatcacca atggaagctc tttatagccc gcatcaagat
    33961 ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa agccattaat tcttccggct
    34021 tactttggtg ctctatcact acataaatat aacccactcc ctcctgcgtt ttcacagacc
    34081 acaagaggtc ggaataatat tgccgcaggt cttcatcaat aaaactgttt ggttctagtt
    34141 taagcgtcgt taaatcacac agtttgcgca gcggcgcggg aagatgaata tcaataaaat
    34201 tccgcgcggt gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc ggcgtggaag
    34261 ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc gacaataacc
    34321 acgattaaca cccaatgcag caggcagttt ttatctttgg acagcgaatt cagaagtatt
    34381 tttagcggac tgcaactttt acatttcagc ggaagctgac tcgcaaattc cccttgaaaa
    34441 tactccggca gcgttagcca ccggagagga aattatttca gcgatccctt aaggaactgt
    34501 tgcagacgag ggctttgcgg gttgccaaat aactgctccg gcgcgccctc ttcttctatt
    34561 ttcccctgat ggaggaaaat gacgtgagta gaaacatggc gagcaaagcc catttcgtga
    34621 gtcactacca ccatggtttt cccctcttct gccagttgct gcataatacg taacacttcg
    34681 cctaccagtt caggatcgag cgccgaggta ggttcatcaa acagtaaaac ttccggttcc
    34741 atcgccagcg cccgcgcgat agaaacacgc tgttgctgac cgccggaaag atgcaccgga
    34801 tatttcccct gcgcacgttc gtctatcccg actttcgcca gatacttcac cgcccgctcg
    34861 cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg cttccatgac gttttccaac
    34921 accgtcatat ggctccagag attgaagtgc tggaatacca tcgtcaggcg tgtgcgcagt
    34981 aagcgcagtt gatttttatc ggcgactttg agttgaccgt ctttgtcgcg caccagattg
    35041 atcgtctggc cattgaccac gatcgatcct tcactcggtt tttcgaggaa gttaatgcag
    35101 cgcagaaagg tacttttccc cgatcccgac gatccgatga tgcttattac atctccggca
    35161 ttcgcttgca gtgagacccc tttcagcact tcgtgttcgc catagcgttt gtgcaaatcg
    35221 ataacgttta atttattctc ggacatcgta ttctcagtgc gttgaagaag gtttcacatg
    35281 ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc aggacataag agatgattaa
    35341 atagagcacc gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg cgttaatatc
    35401 gcgggctatt ttcagcagat ccggcacagt ggccgtaaac gccagcgcag tagagtgcag
    35461 tatcaggatc acttcgttgc tgtatgccgg taacgcaata cgcagcgcag aaggcaaaat
    35521 aatgcagcga tacattttaa aggtcgagaa gccataggct ctggcggctt caatttcccc
    35581 atgcggaacc gaacggattg ccccagcaaa aatctcggta gtgtaagcgc aggtgttaag
    35641 cgtcagcgcc agcacggtac agttcaggcc actgcggaag aaagcgttaa ggaattcggt
    35701 tcccttaaca atctcaagcg tgtacatgcc ggaatagaac accagcaact gaacatacag
    35761 cggcgtaccg cgaaaaatat aggtaaataa ccagattgga aactggatgt atttattact
    35821 ggagacacga ccaatcgcca gaaacagcgc caggactccg cctatcacta ccgacaaaat
    35881 aagcagccac agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc acagcagcgg
    35941 tttccagtat tcatgtaaga tttcgatcac aggtcagccc tcttcacacc cacggagtag
    36001 cggcgctcaa ggaacagcag cacaccattg gaaacggtgg tgaaaaccag gtaaatcacg
    36061 ccacagacga tggcgaaata gaacggttcc caggtacttt tgcctgccag ctgcgtggct
    36121 ttgaccacat cttccaggcc gagtaacgaa accagtgcgg tagatttgag gatcacctgc
    36181 cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat gatccgccga
    36241 aacacttgcc cgcgagtaaa accgaacgcc gtcgccgcct ctatatgccc tttcggcact
    36301 gccataaacg cgccacggaa cgtttcggta aaataagcac cgtagataaa accgagagtg
    36361 ataataccag cgaccatcgg atcgatatca atctgcccga cgcccatcgc ctccgtcacc
    36421 atgtttagcg caatctgcaa accatagaaa atcagcagca tcaacaccaa gtccggcacg
    36481 cctcgaatca gcgtggtgta cccttcgaaa atcagccccg aaagccgatt ttgcgagagc
    36541 ttaccgccag cgccaattaa accgatgatt acagcgagca ctacagagct gatagccagc
    36601 tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa acccatacaa catgctgcct
    36661 gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc gaagggagcg
    36721 ggctttcttt caccgatggc gattaaccac cataaacatc aaaatcgaag tactttttcg
    36781 ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc agtgcctcgc
    36841 gcagttcgtt atcttcttta cgcaggccca tgccggtccc tacgccaaac agtttttcat
    36901 ctttaacaga cggaccaccg aatttgtaat ctttaccgac gggttgtttg aggaaacctt
    36961 cgctggcagc gacctcatcc tggaacgcgg catcaatacg tccggcagtc aggtcagaat
    37021 aaatgttgtc ctgcccctga tacgagacga tttcaatgcc ttttggtgcc cagtgttcat
    37081 taccgaacgt ctcctgggtg gtgccctgca atacgcctac ccgtttgcct ttcagcgact
    37141 cgactgtcgg ctgaatgtca gaatttttcg ccaccaccag acgagaatcg gcagcgtaca
    37201 gtttgtcggt gaaggcaatt tcttgctgac gtttttccgt aatggaaagc gatgacatga
    37261 tggcgtcaat cttcttcgct tttaaggacg ggattaacgc atccagcggg ttttcgacaa
    37321 acgtacattg cgtattgatg cgcttgcaca actccttcgc caactcgata tcgaaaccaa
    37381 ccagttcgcc ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg gtaccgatgc
    37441 ggatgttttg cggaattgca gcaagtgccg cagttgcgct ggagaaggcc agaaccagag
    37501 agagcgatag caccagtttt ttcataatgt cctcaactga cagtcttttt atagggaata
    37561 tttacaggtt tgttacactt atcgtgccat aaaattgacc atcaaggcaa aatatcccgc
    37621 cccgttagac ttattatttt tcattaaaaa tgcttaagta tgcatttcaa tgcaccacga
    37681 tagtgcaaca aacctggcgt gcaccatgat gatcattcca tcaggtacag cttcccagcg
    37741 acgtatcagt caccgtagac attaaagtcg aaatactttt tcgccatctt gtcgtaggtg
    37801 ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc agcatcatct
    37861 ttacgtagcc ctacaccggt gccatcaccg aagtattttt tgtcttttac tgatgagcca
    37921 gcaaaggcga aatctttacc agcaggttgc ttgaggaatc cttcgctggc agcaacttca
    37981 tcttgtaacg cagcatccag acgccctgca gccagatcgg aatagaccaa atcctggttg
    38041 gcataggcca ccacatccac gcctttacta cgccaggtct cgttagcgta agcttcctgg
    38101 gttgatccct gcagcacacc aacatgttta cctttcagtg aatccagcgt tggctgaatc
    38161 ggtgaacctt tggcagcaat caaacgagaa tctgcggcgt acagcttgtc ggagaaggca
    38221 atctcctgct gacgtttatc ggtaatggaa agcgacgaaa taatagcgtc gatttttttc
    38281 gctttcagtg aggggatcag cgcgtcaaag tcactggcaa cctaggtaca tttcacctgc
    38341 atccgtttgc acatctcgtt accgagatcg atatcaaagc caacaaaatc acctttagca
    38401 tctttcgacg agaacggcgc ataggtggta tcggttccga tacgtaccgt ctccggtagc
    38461 gccgcatagc tggaagccgc tgcggagaga ccgactaata aagacagagc gagaatcgac
    38521 ttcttcatac ataaccctca agtgaaaatg gcttttttat gttgtgtatt gtgttgtgtg
    38581 tttgcaggct ctttcatgca ggtcttatgc catcttgacg acaacagtaa cattcaacgt
    38641 taaatatgtt aataagacgt tgcattattg tcctgaagtt gaagatagca ggtatagcgg
    38701 ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt gatcgcggtt
    38761 gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg ccctgccagc gggcaaagag
    38821 atcttcagga agggttattg caaactggtc aagaacacga ttaaccgtct gatttatcac
    38881 atcatcaagg gattgcgggc gatgataaaa cgccggaacg ggaggcataa tcaccgcacc
    38941 gatttctgcc gcctgagtca ttaaacgcag atggcctaag tgcaatggtg tttcacgcac
    39001 gcagagtacc aacgggcgac gctctttcag caccacatct gccgcacggg tcagtaagcc
    39061 atcagtatag ctatggacaa tgccggaaag ggttttgatt gaacagggta aaatcaccat
    39121 ccccagcgtc tggaaagaac cggaagagat gctggcggaa atatcgcgcg catcgtgcgt
    39181 gacatcagct aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg ataaggtctg
    39241 gcgcgccgcc tggctcatca ccagatgcgt ttcgatatct gtgacatcgc gcagaacctg
    39301 taatacgcgc acgccataaa tcgcgccgct ggcaccgctg atgcctacaa tgagtcgttt
    39361 cataaatagt tgccctgtca gacttgcagg cagactttgc agtatttcgg cgggagttgc
    39421 aagtcagggt gccagaccgg caccctcagc gaaggcatca tccttcgtta tgcatttcga
    39481 gattttccac ttcgttctga cgttgcactg ctttggcgtc atcattacgt aacgtatcga
    39541 ggaaatcgag gtagccctga tcaacatctt tggtgacgta gacgccgttg aacaccgagc
    39601 attcaaactg ctggatatcc ggattttcag cgcgaacggc gtcgatcaga tcgttcagat
    39661 cctggaaaat caccccgtca gcaccgatga tctggcgaat ttcatcaact tcgcgaccgt
    39721 gagcgatcag ttccgtggcg ctcggcatat caataccata aacgttcggg aagcgaattt
    39781 ccggtgccgc agaagcgagg tacactttct tcgctccggc ttcgcgtgcc atctcgataa
    39841 tctgctcaga agtggtgcca cggacgatgg agtcgtcgac cagcaggacg tttttatcgc
    39901 ggaactcggc gcggttggca ttcagtttac ggcgcacgga cttacgacgc agctgctggc
    39961 ccggcatgat aaaggtgcgg ccaacatagc ggtttttaac gaagccctgg cggtacggtt
    40021 tgcccagaat acgtgcaatt tccagcgcga tatcacacga ggtttctggg atcgggatca
    40081 ccacgtcgat atccagatct tcccattcgc gggcaatttt ctcgcccagt ttcgtgccca
    40141 tattcacacg cgcgctgtaa acggaaattt tgtcgataaa cgagtccggg cgggcaaagt
    40201 atacatactc aaacaggcac ggattgctga ccggattgtc agcacattga cgggtaaaca
    40261 actgcccttc ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc aggaaatcaa
    40321 agcccagcgt atcgagcgct acgctttcgg aagcgaccat atattctgta cggttctcgt
    40381 caatatcacg ttttcccagt accagcggac gaatcccgtt tggatcgcgg aaagcaacca
    40441 taccgtggcc gataatcatc gccacacagg catacgcgcc gcggattaag cggtttgtgg
    40501 cagcaatggc agcgaaaata ttgtcggctt ccagcgggta gtggcggaag ttgtccagct
    40561 cgctggcgaa gatattaagc agaatttccg agtcggaagt ggtgttgatg tggcggcgtt
    40621 tttcttcaaa cagtttttta cgcaactcgt gagcgttggt cagattgccg ttgtgggcaa
    40681 gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc ttcagaggcg ctggagctgc
    40741 cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc tgcatatggc
    40801 gagcttcaaa tacatcgctc accagcccgt tcgctttacg caaacggaag cagttattgg
    40861 catctatggt gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc gttaaggcat
    40921 cataaatcga ctggttaacc ggcataacac cggcgatacc gacaataccg cacatacgtc
    40981 tttttcctcg ttaagccaca tctcagagca cttacgctct gggcaagaaa cttgacgagc
    41041 tttgcagata atcaaaaaac catctgatga tataactgaa ttgcgggatc agctgtgatt
    41101 tgctccagtc ttcacttttc gacaccccgg taaaggagtc gagaaagaag agaatggcgg
    41161 caacaatcaa cacaccgcgc aacgcaccga aacagacgcc cagcacccga tcggtgcctg
    41221 acaaccccgt tttctccacc aactggccta tcacgaagtt cacgatagca ccaacgatca
    41281 gggtagcgat aaacagtacc gcgatggcaa tcccatttcg aaccagttcg tcttcaaagc
    41341 ccgtaaacca gactgacagg taagtgtagt aatgactggc aacaaagaaa gcacaacccc
    41401 atgtcaccag cgataacgct tcacgaacaa agccgcggat caggctaacc agagaggaaa
    41461 aagcaatcac cgcgattatg gcgtaatcaa tccagaccat atgtgtccca cgattttacg
    41521 ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc gtagggattt
    41581 ccttcccgcg catcaataaa aatggcgctg aaaaaatatt caacgccatc gactttttat
    41641 gcctttgcgg catcgtgcaa tgcgtgtcgg atgcggcgta aacgccttat ccgacctacg
    41701 gttctaacac tgcccaagcc tgataagacg cgccagcgtc gcatcaggca agactgtatt
    41761 aattcggcgt atagcccatt accacgccac ttaagccaga aagttgcttc aactcaccca
    41821 gcgaaccttt cagcttatct ttcgaggcat ccggcccaac cagaatacgg gtaattttac
    41881 cctgcactgg cgtggatggc gacgtataaa cccgataacc ggcaccgcgc agcttaccga
    41941 caatctcatt cactttatcg gcatttttca gcgcacccag ttgcacaaca taggctttac
    42001 ccgttggcgc tgctttttct tccacgaccg gcttcggttc tggcttcggc gcaagtggtg
    42061 cttcaacctt tggtttaggc ggctccaccg gtttcggctt cggtggggcg accggtgcag
    42121 gttccggttc aaactcggtg ttattggctg caatagtggc cggatcgagc gacggtgctg
    42181 cggcatcacc tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc ggcaacgcct
    42241 gggtggcggc tggcatcata tcaggctcat cacgatcgcc cgctttcggc accagcggga
    42301 tagccgcgaa ctcatcctga taatgttttt tctgcccgtc cagcagccct ggaagtacaa
    42361 tcacccccag cgccaccagc acgatcgtgc ccactaaccg attctgaaac ttacttgcca
    42421 ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa agaaccacac
    42481 accagcacgg tgtcttccgc tttagcgtcc gccattgcgg catcccatgc ctgcgcaacg
    42541 ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt gttctgccgt ggcaccgcgc
    42601 ggcccttcca gtggcgcaca ataccagtca tcaaccacgc ttttcaacca ggccagagtt
    42661 ccggcaatat ctttatcatg tagcataccg ataaccgcca gcacgcgccc gtttttcggt
    42721 agcgctttca tacgcccggt gagatattcc gccgcatgtg gattatgcgc gacatcaaaa
    42781 ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc gctggcaatc
    42841 ccgtcgcgaa tggcattttc actgacttcc agcccgctgg cacgcagtgc cgccagcgcc
    42901 gttgcggcat tcggttgcgg gacaagcggc aacggcagat tttccagcgt gccgtgagca
    42961 tcgctaaacg cccagtcatg atcggtgacg gaatagttcc actcaacgcc ccgacgttgt
    43021 aacagtgcac ctttttcctg cgccacatca gcaatggtag aaggcatttc cggctcaccg
    43081 acaattgccg gtttttcgct gcggaagatg cctgctttct cgcgaccaat actttcgcga
    43141 tctggaccca gccagtcggt atgatccagc gcaatactgg ttactaccgc gacatcggca
    43201 tcgacaatat tggttgcgtc cagacgaccg cccagcccta cttccagaat caccacgtca
    43261 agttgtgcct gcttgaacag ccacaacgcc gacagcgtac cgtactcgaa ataggtcagg
    43321 gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc cgattccagc
    43381 aattcctggc cctgcacacg tacgcgctcg gtataacgca ccagatgagg cgaactgtag
    43441 acgcccactt tgtaccctgc cgccatcaga atcgactcca gcgtacggca ggtggtgcct
    43501 ttgccattcg tacccgcaac ggtaaacaca aatggcgctg gtttcaggac gccaagacgc
    43561 gccgcgacct ggctcacgcg ctcaaggccg agatcgatag ttttactgtg caggttttcc
    43621 agataagaaa gccacgaagc cagaggcgac gcggcttgag gagtgcgttt gataatcatg
    43681 gtatccgctg attcgttacg gtgagaatag caaaagggca gagccagtgg ccctgccctt
    43741 atcagttatc aggcctcagg ttcctgatcc ggtaccgggg gtaccactac gccttcacgc
    43801 ggcgcttcag gattcggcgc tggcagattc atcaacttcg ccagaatgct cgccagtttc
    43861 aggcgcattt ccggacgacg gacgatcatg tcgatcgcgc ctttctcgat caggaattca
    43921 ctgcgctgga atctaggcgg cagtttttcg cgaacggtct gttcgataac acgcggaccg
    43981 gcaaagccga ttaacgcttt aggttcagcg atgttgagat cgcccagcat ggcgaaactt
    44041 gcagaaacac cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa gccgcgctcc
    44101 tgcatttttg ccagtgccgc agaggttttc gccatctgca tcagcgacat cagtgcttcc
    44161 tgcatacgtg cgccaccaga ggcggagaag cagatcagcg ggcagttatc ttcctgcgcc
    44221 tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc ccattgaacc gcccataaag
    44281 gcgaactcga atgccgcagc gacaaccggc attccataca gagtgccttt catcaccacc
    44341 agcgcatctt tttcgccggt ttctttctgc gcagatgcca gacggtcttt atacttcttg
    44401 gagtcacgaa acttcagctc atctttcggc tcaagctcgc tacccagctc cacaaggctt
    44461 ccttcatcta acaggctatg caggcgatta cgcgctgtca tacgcatgtg atggtcacac
    44521 ttcggacaga cctcaagatt acgttccagc tcagcgcggt ataaaacctg accgcagcta
    44581 tcacacttag tccacacccc ttcaggaatg ctcgccttgc gggtgggagt aatgttgctt
    44641 ttaattcgtt caatccagct cattagggac ctttctgtct gaacctagtt cgatgccagt
    44701 tttatctttg gggacgcata atgccatttt tgcccccaac agaccatgaa tgttgcacat
    44761 taaaacataa cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga gttacttttt
    44821 attttgcggc gcgtgccgca gcgcgtttgt gacggattat ttcgatgacg cccggcaaaa
    44881 tagaaaccac aataatcccg acgatcagca gcttaaggtt atcctgaacc atcgggattg
    44941 taccgaagaa atagcctgcg taggtaaaaa gcagtaccca caacagtgcg ccgatcacgt
    45001 tataagcagc gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac ggggcgaacg
    45061 ttctgacgat cggcacaaaa cgggcgagaa taatcgtttt gccgccatgt ttctcataaa
    45121 actgatgggt tttgtcgaga taactgcgac ggaaaatttt cgaattggga ttactgaata
    45181 acttttcacc gaacaaccgc ccgatagtgt aattgaccgc gtcacccaca atcgcggcga
    45241 tcagcatcag caccaccatc atatggacgt tgagatcgtt ggtttccagt gatgccaacg
    45301 ctccggcaac aaacagcaat gaatcacccg gtaaaaacgg cattaccacc agaccggttt
    45361 cacagaacaa aattaagaac aaaatggcat aaacccagac gccgtactcc gcgactagct
    45421 ctgccagatg cacgtcaatg tgcaggataa aatcgatgag gaaataaatc aggtccatat
    45481 ttgcctatgc cttgtactcg tcatctttca ggctgtaact acgttggcta cagcctgaaa
    45541 tgttccgagc attattctcg ttagtccgcc agaaatagcg ggcccattgg cggttttgga
    45601 agatcatacc ggtcagggta atccaccgcg accagataca gcccttccgc ttttgccgtt
    45661 gctgccgcca gcgttctgtc ctttgccgcc agcaactctg ctatccagct ctccggctgg
    45721 ttgtgggcac cgacttccat caggctgccg acaatattcc tgaccatatg atgtacaaag
    45781 gcattcgctt tgatatcaac caccacataa ggaccgtgac gcgtgacatt aatatgcata
    45841 acgttgcgcc acggggttcg ggactggcac tgcaccgcac ggaacgaggt gaaatcattc
    45901 tcgcccagca agcattgcgc agcccgatgc atccgttcag cgtccagcgg ttcgtaaaaa
    45961 tgggttaccc ctttactcag taccgccggg cgcagccgat gattgtagat gatgtagcga
    46021 taacggcgag ccgtggcgct aaatcgggca tgaaaatcat caggtacagc tttaacccaa
    46081 cgcacggcga tgtcaccagg taaattcgca tttacgccca aggtccacgc cgcgtctttg
    46141 cgctgcgcgg ttgtttcgaa atgcaccact tgcccggtac cgtgtacccc tgcgtcagta
    46201 cgcccggcgc agaagacggt gatgggttcg ttcgccacct gggagagcgc cttttccagc
    46261 ttctcctgca cactgcggac ttcattctgc cgttgccagc cgtaatactt actgccgtcg
    46321 tactcaatgc ccagcgcaat tttataaact ggcagttgtt gctggtcgga cattagtaca
    46381 gatactcctg caccagtttc tcggcgattt ttactgccat cagcgcgccg ccaaagcgaa
    46441 cgttatcggc caccgaccag aactggactt gctccggcat accgtagtca ttacgcacgc
    46501 agccaacaga aagatgcggc gtacccgaag catcccctac ctgagtcggg aattcgttct
    46561 cttcagagag cacaatatct tcgccctgag caaacgcatc acgcgcttct tctgccgcca
    46621 gcggacgcag tgcttcaaag ttgaccatct gggcatgacc gtagaatacc ggtgcctgga
    46681 cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat tttgcgtact tcgtcaacga
    46741 tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa caacggcagc atattgaacg
    46801 ccagttgacg accgaagaaa tcttcttcgt caatcggaat gccgttgagc aatttcgcac
    46861 tctgccccgc taacgcatcg accgcttttt tgccctgggc ggaggctgaa atcaggctgg
    46921 taacactgat acgcgacaaa ccgccctgat cgattaacgg tttcagtgcc gccagcagct
    46981 ggctggtcag actgtctggt acggcgatga cattccggtt gcggtaatcg gtcagtacaa
    47041 acgggtttac ttccggcacc accagcggta cgtcgggttc gagagcaaac agtccgctgc
    47101 tgtcgatcac caggcaacct gagttggtcg cttcttcaac ccaggcagcg gtagcttctt
    47161 tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc gaattcagcg gcatcctgca
    47221 cggtgattgt tttaccacca aagcgcagtt gttcgcctgc gctttcgtta cgtgccagtg
    47281 cataaatttc cccaaccggg aactgacgtt cagccagcgt ttcaagcagg gcttcgccca
    47341 tagcgccagt tgcgcccagg acggcaatgt tccagccttc agacatggtg gtttactcca
    47401 gaaatagcaa agctccctgc caatgttaca gcagagagca tgaagaagag attaacgtgc
    47461 cggatgatga acggcgttaa aacccagttt acacagcaat gatgccgcac tggcgtcatc
    47521 acaaattaca tacagagatg accattcacg gcgctcaaga tagtttttgc gcagtttatc
    47581 gaactcaccc ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc gcacatcata
    47641 caccaaatgc accagccttt tcagcgtcgg ttgatcgagc ggaccatgca gcgtaatgcg
    47701 gccaaactca ggcgcgggca gcaacgtatc cagcgcaacg tgttgttcat gcccaataaa
    47761 cttgctatag gcttcaaaca cttgcgtcgt gccacgcgct ttaccctcca gggtatagcc
    47821 tgcgatatgc ggcgtgccga tatccacttt tgtcagcaat tccacattaa gttccggctc
    47881 gccttcccag acatccagca ccacgcttaa cttctggcct tcattcaggc aggtcagcag
    47941 cgcggtatta tcgacgactg cgccacggca ggcgttaatc agaatcgctc cgggcttcag
    48001 gctacggatc agtttttcat ccgccagatg tagcgttttg tacggaccat ctttaaagag
    48061 tggcgtatgg aaagtcagaa tatccgcgcg ctggactaac tcatccagcg agcggaaatc
    48121 accctcatcc ccacggtcgg cgcgaggcgg atcgcaaagt aaggttttaa tccctaacgc
    48181 ttccagtcgc acctgcaatc gacgtccaac gttaccaacg cccacgatcc ccaccgtacg
    48241 gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagc agggaggaga aaacatattc
    48301 caccaccgca atcgcattac agccaggtgc agcggaaaaa ccaattcccg cctgcttcaa
    48361 ccatgcttca tcgacatggt cggtccccgc agtggcagtt ccaacaaatt taatgggttt
    48421 tcctgccagc aaagattcat tcactttcgt gaccgaacgc accatcagcg catccgcgtc
    48481 tgccagttga gcgacgggga ttggacgccc gggaaccgcg atcacctcac ccaaacggct
    48541 aaataagtcg cgggcataag gcatattttc atcaacaagg attttcacgt ttgtgttacc
    48601 tgtatgagac gagagttaac cggacaagtg tgccataatc tcgcggccag gcatacttgc
    48661 gaagatttca ggtataagga tacgtaatga tacaacctat ttccggccct cctcctaggc
    48721 agccaccagg tcagggagat aacctgccgt ctggcgcggg caatcagcct ttatccagtc
    48781 agcaacgtac ttcgctggaa agcttaatga cgaaagtgac ctcactgacg caacaacaaa
    48841 gagcagaact gtgggcgggt atcaggcacg atattggtct gtcgggagat tcaccgctgc
    48901 tttcgcgtca cttccctgcc gctgagcata atctggcgca acgtctgctg gccgcgcaaa
    48961 aaagccattc tgcccgccag cttttagcgc aattagggga gtatttacgt ctggggaata
    49021 atcgtcaggc ggtcacggat tatatccgtc ataactttgg tcagacgccg ctgaatcagc
    49081 tctcaccgga gcaattaaaa accattctca ccctgttgca ggaagggaag atggttattc
    49141 cgcaaccaca gcagcgcgag gcgaccgacc gtcctttatt accggcggag cacaatgcgc
    49201 taaaacagct ggtgaccaaa cttgcggcgg caacggggga acccagcaaa cagatctggc
    49261 aatcgatgct ggaactttcc ggggtgaaag atggcgagtt aattccagcg aaactgttta
    49321 accatctggt gacctggcta caggcgcgtc agacgctaag ccagcaaaat acgccgacgc
    49381 tggaatcact acagatggcg ctaaaacaac ctttagatgc cagtgaactg gcggcgttat
    49441 cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct tctgcccagg
    49501 ccgaggatat tcttaatcag ctttatcaac ggcgggttaa agggattgat ccgcgtgata
    49561 tgcaaccgct gcttaatcct tttccaccga tgatggacac gttgcaaaat atggcaacgc
    49621 gtcccgcgct gtggatactg ttagtcgcga ttatcctgat gctggtctgg ctggttcgtt
    49681 aaccccgacg aaatgacagt atcgtgacaa taatacccag caccgcagag atcgccccgg
    49741 caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat tcccgccagc ggcccggaga
    49801 cgccgagggc gatatcctga aacgcggcgt aaccgcccag tgcggtgccg cgaacttgtg
    49861 aggggacgcg tttaaccacc tccacgccca gcgcaggaaa gataagcgaa catccggctc
    49921 cggttaacgc cgcgcccgct aatgcgaccc atgcacctgg ggcttgccag agcagcaaca
    49981 agcccaccgt ttctacaagc agagagacaa tcgccacttt cacgccgcca aaacggtccg
    50041 gcatccagcc aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg gtaagagtaa
    50101 agcccgccat cgcccatcct ttgctggcaa agtagagcga aacgaaagtc ccgataaccg
    50161 caaaaccaac gccttgtagt gccagcccta accctggttt ccagataagc ccgacaacgc
    50221 tccacagcga tggacgttct cccgccaggg ccggtacttt gcgcactgtg ccgttacagg
    50281 cccacgccag taagggtaat accattgtgg tgatcgccag tgcggcaaaa ccgtaatggc
    50341 tatgaatcaa caggccaagc ggcgcaccaa cagcgagggc accgtaaatc gccattccgt
    50401 tccatgacat cactttgcca gagtgtttta gccctacgat gcctaacccc caggtcagag
    50461 cgcctgtcag tgactggctt tcaccaaacc caagaatcaa acgcccgatg accaacaggg
    50521 caaatttgaa cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg ccgccagcca
    50581 gaccacaagc taacatcccc tgaagcgccg aacgttttgc gccatattga tcggccagtc
    50641 gcccggcgta accacgcgtc agcaccgtag ccagaaactg aatcccgacg gcaatgccga
    50701 ccatggtatt gccatagccc agatcatgat gaacaaacag cgggataacc ggcaacggca
    50761 accctacggt catgtaggtg agaaaaaccg caaaagcgat gcggaagagc gaaaaactgg
    50821 cagaagatcg tgtttcggtt tggcttacag cagtcatgca ttactccaga atgcagcgca
    50881 aggcgaggag tatccccgtc tcatctctct ggtttcaggg ttacggtgcg ttggcaggat
    50941 ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc tggaactggt
    51001 ggcgattgtc aatgatgtga aaaagggaac catcaggttc ccttttgcgt tagtgccgga
    51061 ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg tcttatccga cctactccgg
    51121 cctactgcga attaatcttt cagcttgcgc attaccagcg tggcgttggt gccgccgaag
    51181 ccgaagctgt tagacataac tgtggtcagt tcgcgatcgg tcgtttcggt cacgatgttc
    51241 agacccgcag cctgctcgtc cagctcttca atgttgatgc tcggggcgat aaagccgtgt
    51301 tccagcatca gcagagagta gatagcttcc tgcacacccg cagcgcccag tgagtgaccg
    51361 gtcatggctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc acggatagct
    51421 gccagctctt tcacgtcgcc aaccggagtc gaagtaccgt gagagttcag gtaatcgatt
    51481 ggggtatcaa cgccatgcat cgccatctgc atacaacgta ctgcgccttc gccagacgga
    51541 gcaaccatgt ctgcaccatc agaggttgcg ccgtagccaa cgatttcagc atagatgtaa
    51601 gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta ccataccgcc gccgccagcg
    51661 ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg ggtgtcgttg
    51721 tatttagtag acagcgcacc catcgcgtcg aattcgcagg ccatttccca gcacagctct
    51781 tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc tactgcgtta
    51841 ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac gccatgaatt
    51901 ttaaacggcg tggcgagaca ggcagaaacg ccggatgcca tcgctttagt gaccacatac
    51961 gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa cacctggaaa
    52021 cgcggggaac cgccgccgga acctgcaatc aggccaacgc gcgggttatt ctggtaagct
    52081 tccggagaga ggcccgcatc agcgattgcc tgctccatag aaaggaatgc ataaatggat
    52141 gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc cagttttacg
    52201 ttgccccaga cgtggctacg catgccggaa tccttcagct cctgagagaa agtgatccct
    52261 gaacgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat gctggaaaca
    52321 atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc gcacatagag
    52381 taagtttcga atgcacaata gcgtacactt gtacgccgaa caagtccgat cagccattta
    52441 atagagaaat ttgcgcagcc ttacacacat cgctaagatc gagccaccgc ctgtaagacg
    52501 agtaacttac gtgaaacact actccataca acctgccaac ctcgaattta atgctgaggg
    52561 tacacctgtt tcccgagatt ttgacgatgt ctatttttcc aacgataacg ggctggaaga
    52621 gacgcgttat gtttttctgg gaggtaacca attagaggta cgctttcctg agcatccaca
    52681 tcctctgttt gtggtagcag agagcggctt cggcaccgga ttaaacttcc tgacgctatg
    52741 gcaggcattt gatcagtttc gcgaagcgca tccgcaagcg caattacaac gcttacattt
    52801 cattagtttt gagaaatttc ccctcacccg tgcggattta gccttagcgc atcaacactg
    52861 gccggaactg gctccgtggg cagaacaact tcaggcgcag tggccaatgc ccttgcccgg
    52921 ttgccatcgt ttattgttcg atgaaggccg cgtgacgctg gatttatggt ttggcgatat
    52981 taacgaactg accagccaac tggacgattc gctaaatcaa aaagtagatg cctggtttct
    53041 ggacggcttt gcgccagcta aaaacccgga tatgtggacg caaaatctgt ttaacgccat
    53101 ggcaaggttg gcgcgtccag gcggcacgct ggcgacattt acgtctgccg gttttgtccg
    53161 ccgcggtttg caggaagccg gattcacgat gcaaaaacgt aagggctttg ggcgcaaacg
    53221 ggaaatgctt tgtggggtga tggaacagac attaccactc ccctgctcca cgccgtggtt
    53281 taatcgtacg ggcagcagca aacgggaagt agcgattatc ggcggtggta ttgccagcgc
    53341 gttgttgtcg ctggcgttat tacggcgcgg ctggcaggta gcgctttatt gcgcggatga
    53401 agcccccgcg ctgggtgctt ccggcaatcg ccagggggcg ctgtatccgt tattaagcaa
    53461 acacgatgag gcgctaaacc gctttttctc taatgcgttt acttttgctc gtcggtttta
    53521 cgaccaatta cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt taggctggga
    53581 tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg gatttacccg cagaactggc
    53641 tgtagccgtt gaggcaaatg cggttgaaca aattacgggc gttgcgacaa attgcagcgg
    53701 cattacttat ccgcaaggtg gttggctgtg cccagcagaa ctgacccgta atgtgctgga
    53761 actggcgcaa cagcagggtt tgcagattta ttatcaatat cagttacaga atttatcccg
    53821 taaggatgac tgttggttgt tgaattttgc aggagatcag caagcaacac acagcgtagt
    53881 ggtactggcg aacgggcatc aaatcagccg tttcagccaa acgtcgactc tcccggtgta
    53941 ttcggttgcc gggcaggtca gccatattcc gacaacgccg gaactggcag agctgaagca
    54001 ggtgctgtgc tatgacggtt atctcacgcc acaaaatccg gcgaatcaac atcattgtat
    54061 tggtgccagt tatcatcgcg gcagcgaaga tacggcgtac agtgaggacg atcagcagca
    54121 gaatcgccag cggttgattg attgtttccc gcaggcacag tgggcaaaag aggttgatgt
    54181 cagtgataaa gaggcgcgct gcggtgtgcg ttgtgccacc cgcgatcatc tgccaatggt
    54241 aggcaatgtt cccgattatg aggcaacact cgtggaatat gcgtcgttgg cggagcaaaa
    54301 agatgaggcg gtaagcgcgc cggtttttga cgatctcttt atgtttgcgg ctttaggttc
    54361 tcgcggtttg tgttctgccc cgctgtgtgc cgagattctg gcggcgcaga tgagcgacga
    54421 accgattccg atggatgcca gtacgctggc ggcgttaaac ccgaatcggt tatgggtgcg
    54481 gaaattgttg aagggtaaag cggttaaggc ggggtaatct gctctcgcag tgtttgccgg
    54541 atgcggcgtg aacgccttat ccaacctacg tgtgacgtgt aggcctgata agacgcgata
    54601 agcgtcgcat caggcatggt gctccagacg ccgcaactta cagctgtgac gcctgctgaa
    54661 acaggttttc ccacatatcg gtcaccagcg cctggtcacg cggcgacaat tcaccggcac
    54721 caatggcttt ttccagactc tggctaacgg tcgtatgcac cgcctgagcg gagtggtcat
    54781 caccactttc cagttctgcg atggctaatg tcaggtggcc acgcaaatac ccactggcaa
    54841 acaactcatc atcactggca tggtcaacca tgccgtcgat taatgccaga atgcgtgatt
    54901 caaactccgc gatcatcttc tttcctcatt gtaaaacgtg gcgctggctt cagttgagcg
    54961 cttccggcca cgggaactgt tccgccgtaa gttccggcgt gtgataataa ttttgtagtg
    55021 ctttaatgaa gcgtgccggg cgttccggaa tgccatttgt cagataatcc atcacctgcg
    55081 catgtacccg ccgttgaaac accacacgat ccggttcgaa atccccttcc agattgtcgc
    55141 aactgacgtt aaacggatat ccagccgcca cgcagaataa ccagtcgaac gcctgcggct
    55201 tcacttcaac atcttcaaac tggctttgcg tctgggcatc gcgtccgtcc gggcaatacc
    55261 agtagccgaa atcaaccagc tcacggcgcg cttttccggc aatacaccag tgcgaaatct
    55321 catgaattgc gctggcataa aagccatgag caaagacgat tcggttatac ggtacttccg
    55381 catcagcagg aagatagatc ggttcgtcgt cgcctttaat cagacgggta ttaaattcat
    55441 cggcaaagca gctattaaaa atttcaatca actgctcgta gtggtgtgta ctgttcatta
    55501 gttcatcccc aaccagtgga ggatctcctg tccgtggcta tcgtaaagta atttggcact
    55561 catcaccgcc gagacgataa caatcatcgg gcggatcagc ttttgtcctt tgctcaacac
    55621 caaccgtgaa cccatgcgcg cgccgaggaa ctgccccacc agcatgacaa agcccgtcgc
    55681 ccaaatcact ttgccgccga gaataaacag cagcagaccg ccaatgtttg acgttgcgtt
    55741 gagtaatttg gcgtgagccg tggctttggc gaggttgaat ccgcacagcg taacgaaggc
    55801 cagcgcgtaa aacgatccgg cagccgggcc aaagaatccg tcataaaaac cgacgcagcc
    55861 accggcaatt aacgcgaagg gtaaaccgta catccggcgc tggcgatatt cttcacccag
    55921 ctttggcatc agcaagaaat agaggccgat acaaatcacc agaatgggca aaatctgccg
    55981 taagacatca gcctgaacgt actgtaccaa cagtgcgccg ctcattgagc cgacaaaggt
    56041 catggcaata ttgagtttct gatcgcttaa actaaccact ttgcggcgaa taaagtagat
    56101 agtggcggaa atagagccgc cgcaggcttg cagtttattg gttgccagcg cgttagcggg
    56161 agacattccc gcagccatca atgccggaat ggtgagtaac ccaccgccac cggcaatcga
    56221 gtcgataaat cccgccagca tggcgacaaa aaagagaact cccagcaaca gcggagaaac
    56281 cataaacagg ctattaaacg tttccattag atcacgtgct catccagtag cgcctggcag
    56341 gaaggcggca acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg ttttggaggt
    56401 tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg atggcggtaa aggttgatct
    56461 tcacactcca gactatcggc agggcaacgt aatcgtacat gcatatgcgc gcgatgctgg
    56521 aaccagggtc gcactttgcg caaccagtcg cgatcggtgc ccgcatcaag gcaaagttgt
    56581 tgtttaatcg ccggattaac aaaaatgcgc gtgacgtctt tgtcctgggc ggcgagtttg
    56641 atcaagctga aaatttctgg cttccacagc gtggagacaa cgtgtttacc gtctcgggaa
    56701 actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg tccagcgagt tttcggcagt
    56761 tgcagaaaga tatcgacatc cagtccggtc tggtggctgg catgaccgcc gttgaaacgc
    56821 ccaccagcgg gcatccccat atcgccaatc agcaccgtac ccatgcccag attgctcacc
    56881 tggctactca gacgctggat aaacatcacc agatccgggt gaccgaaata gcgacgctga
    56941 tcggtacgca tgacctgata atgttcggac tgtatcggca acgtgtcagc gccgacaata
    57001 cagccattag aaaaactgcc tatcgattgt gcgctacccg gcacaggttg ggttattttt
    57061 tgccacggcg tcgctgccag gctagcgcta ctggcaagca gagccagcag cgcaatcgcg
    57121 gttttgttca ttttttacca gcgtggaata tcagtcttca catcggcatt ttgcgcccgc
    57181 tgccgtaaca ggtgatccat taaaacgatc gccagcatcg cttcagcgat cggcactgca
    57241 cggatcccga cacagggatc gtgacggcct ttggtgatca tctcaacttc ttcgccaaag
    57301 cggttaatag tgcgccccgg cacggtaatg ctggaggtcg gtttcagcgc catatgagca
    57361 atgatttgct gcccgctgct gataccgccg agaatgccgc ccgcatggtt gctctggaaa
    57421 ccgtctttgg tgatttcgtc gcggttctgg ctgccgcgca gcgccactac gtcaaaaccg
    57481 tcgccaattt ccacgccttt caccgcgttg atgctcatca gcgcatgggc gatgtcggca
    57541 tccaggcggt caaagaccgg ctcgccaagt ccggcgggga caccactggc aacaacggtg
    57601 actttcgcgc cgatggagtc gccctctttt ttcagcgcgc gcatcagttc atctaacgcg
    57661 tcgattttgt cggggtccgg gcagaagaac ggattttgtt caaccagcga ccagtctttg
    57721 atttccagcg gaatgtcgcc catctgggtc aggcagccgc ggatttcaat accaaatttc
    57781 tcggcgagat attttttggc aatcgcccct gccgccacgc gcatggcggt ttcgcgggcg
    57841 gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt tttgttcgta ggtgtaatca
    57901 gcatggcctg gacggaagac gtctttaatt gcgctgtaat cctgagaacg ctggtcggta
    57961 ttttcgatca acaagccaat gctggtgcca gtagtaacgc cttcaaaaac accggagagt
    58021 attttgacct gatccggctc gcggcgctgg gtggtatagc gcgatgtccc agggcgacga
    58081 cgatcaaggt catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg aacaccatcg
    58141 acgatgcagc cgagcgccag cccgtgcgat tcgccgaagg tggttacgcg aaagagttgt
    58201 ccaattgtgt ttccagccat cacggctccg ttattgttgt gtttgcgtgt ttacttaatc
    58261 tttataaatc gcgaaatgtt ctcgtgcggc aataagctgc tctttagtga gcataaacac
    58321 accatcgccg ccgttatcaa actccagcca ggtgaacgga acatcgggat attgttccat
    58381 aagatgtacc atgctgttgc ccacttcaca aatcaacacg ccatcatcag caaggtaatc
    58441 tgccgcgtta ccgagaatgc gacacgtcag tttcaggccg tcggtgccag atgccaggcc
    58501 cagttccggc tcgtgacggt attcgtttgg caggtcggac atatcttccg cgtcgacata
    58561 cggcgggtta gtgacaatca gatcgtattg cactttcggc aaatcacgga acagatcgga
    58621 acgaatcgga atgacattgt ggatcagacc gtgttcttcg atgttctgtt cggcaaccgc
    58681 cagcgcgtcg ggagagatat ctaccgcgtc gacttccgct tccgggaagg cataagcaca
    58741 ggcaatggcg atgcagccgc taccggtaca catatctaaa atatgctgcg gttgcttgct
    58801 gatgagtccg gcaaatttat tgttgatcag ttcaccaatc ggcgagcgcg gcaccagcac
    58861 gcgttcatcg acgtaaaatt catgaccgca gaaccaggct ttgttggtca ggtaagccac
    58921 cggaatgcgc tcgttgacgc ggcggatcac gcgttcaaca atacggtgtt tttcgctgga
    58981 ggtcagacgc gcggtgcgca tatcttccgg gatatccagc ggcaggtaga gcgatggtaa
    59041 caccagctgt acggcttcat cccactggtt atcggtgccg tgaccgtacc agatatttgc
    59101 cgcgctgaag cggcttaccg accagcgcaa catgtcctga atggtttgca gctcatttac
    59161 tgcttcatca acgaaaattt tatccacgta ttcctccagg gcatgatcgc aataatttcg
    59221 gcggctagtt tgccatgaag atgacgataa atcagcattc acgcgcggtg agtgaggaaa
    59281 aatacgttta aaacgatcga ttgtgctacg agtcgggtaa actgtaggaa aattagaaat
    59341 agagacagat aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc tctgtttcgc
    59401 cagttgatgg cggggactcg caagattaag caggacacga ttgtccaccg accgcagcgt
    59461 aaaaaaatca gcgaagtgcc ggtgaaacgc ttgatccagg agcaggctga tgccagccat
    59521 tatttctccg atgagtttca gccgttatta aataccgaag gtccggtgaa atatgttcgc
    59581 ccggatgtca gccattttga ggcgaagaaa ctgcgccgcg gcgattattc accggaattg
    59641 tttttggatt tacacggtct gacgcagctg caggccaagc aggaactggg cgcgttgatt
    59701 gccgcctgtc gccgcgaaca tgtgttttgt gcctgcgtga tgcatgggca cgggaagcat
    59761 attttgaagc aacaaacacc gctgtggctg gcacaacatc cgcatgtgat ggcatttcac
    59821 caggcaccga aagagtacgg cggtgatgcg gctttgttgg tgttgattga agtggaagag
    59881 tggttgccgc cggagttgcc ctgaatgctg gcagcaaatg ccggagcagc gtaaacgcct
    59941 tatccggcct acaaatatgt gataattcaa taaattgcaa ttcacttcgt aggcctgata
    60001 agacgcatca gagcaatact acgaacttca gttcattcaa tacatactga aaacgacacg
    60061 tcgaatcaat cggttgccca ctactggtat tcacattcac cacaatatcg tgtagtactt
    60121 tatccgttac cggatgacga gttggcgtaa ccgttaccgt tgatttatca caaccgtaag
    60181 aggtaaaggt taacaattct gataccgcgc ggcagtatgc tcctctgcca tcattatcag
    60241 aaaaattact gccatcacag agtctgccgc cttccatcag gtaggtcgaa agcgtggtgg
    60301 tcactgttga ccccgtggac tgaattcgta acgccttatt atggctgaca taattttcac
    60361 tccaggagat atcgtatgaa gcgaccggta tcgttgggca tattgtgtta tgcactgtag
    60421 gggccgtata gcttccaaat ccgggtaaat ctgtatagga ataaaaggat gcgatccaag
    60481 ggcaaccata atcatcagat gcaacggtaa ttttaaacgt catatatgca caacgaaaaa
    60541 ttttcgtatc tatgccaggg caatttgagt tattaattgt atcaaagtca gtcagtacca
    60601 aagttggagc tgaacccgac ccagaccagg aaataacccg caacgtatcg cctttatacg
    60661 caacagaatc atatttccca tatgcaacct cctttggcgt tatcaccgta acattgaaaa
    60721 cactgctcgc tacagcaccg gttaccttgt ccatggcata ataaactctc acactgtcaa
    60781 aactcgcact cataagttta tctccagcat aactcttaga tgcgccgaca acactcagaa
    60841 cgagcagcat taggctcgcg aaaattatgg tccatttttt catttcagaa atactcaaag
    60901 gttgccgtca ctgtggcact aaagtctccg gacgttacat ctcgcccgct ctttgcctgt
    60961 aaccaggtat taaaatttag gctgttattt ccctgattca gcgggagcgt gacgcctgtt
    61021 gtattgttga ctggcaccga tgtgcctgcc gcagtttcaa taccaatccc agcgccggat
    61081 gctgatgacg aggagtcaaa ggccagaaag ccggatagag cactatcttc tgttcctgtc
    61141 aacgttactt tgacattaaa caatgtagaa ctatgacaat ctttcaattg aaacaccaat
    61201 ggcactcgct ctgactgccc aaggttcatt aaatcgctgg cggcaatggc aggaaaatgt
    61261 acttctgcca actggctacc attaatcact ggcgtacacg atttactgat taaattgccg
    61321 gtaaagtgca aattatctcc tgctattgtg gttccactgc ccagcaacat caggctacta
    61381 ataaaaaaca acgtacgctt tttcttcatt ggtaatctac ctgtaacgtg gcccaggcct
    61441 caaagtccgc ctctggcaga acgacaccac tcttttttac tggcacagcc gttaacaccg
    61501 gtaaatcggt ttcattcacc acaagtggcg tattgatcgt aaaactctgc cctgcctgtt
    61561 tgagctgaat gcctaaacca gtgatattgc tgcttactgc agcattatca aaatcgcttg
    61621 gtgtgccact ccagctcaag gtcatttcca ttaatgggtc gcgaaccgcc gtatcgcagg
    61681 taatggaata cggcacggtt tgctcataat tgctgccatt aatgtcatca ataagcacat
    61741 cctgaaaagt tatttcaatg gtttgagcgt tgctaatcgt gcagttaggc ggctgcacta
    61801 aataaccgtg aaaagagacg tttgagagtg ccatcgagca aaagccccac agcaaaataa
    61861 gcgagatccg tttcattggt aatccaccac aattgtggca ctggcgttaa attcagctgc
    61921 cgctaacgtt gtaccactct gtttcaccgg cacagcttcc agaacgggaa ctcccgagga
    61981 aaaattaaat ggcaaccatg agccagaggt taagtcgagt attgtgtgat cactactttt
    62041 ctgaacccga ataccaaacc caggaatacc cgtgctgata actgtttcac cgttaatcac
    62101 gaccgttgtc gcctgcatct gtagacgcag gtcatccatt gccagattgt tacaaacaag
    62161 cgagtagtca atgggacgtt tataatcaac cccattgatc ttattaatga atacattacc
    62221 aaactcaact gccgcaccat ttaccgtaca aggaggaggg gcatcgacaa gcacccgtaa
    62281 attcatactt actgacttac tcgctgcaac tgcaaagccg ctaccaacac aacatatcag
    62341 taacccagtt accgcccaga cagaacaaga tcctttcata ctggctctcc cggcctcagc
    62401 ctttgcggct ctgctcttca tc
//
LOCUS       DADLSA010000007        61885 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.007, whole genome shotgun sequence.
ACCESSION   DADLSA010000007 DADLSA010000000
VERSION     DADLSA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 61885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.007"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..758
                     /gene="yegD"
                     /locus_tag="JN691_000458"
     CDS             <1..758
                     /gene="yegD"
                     /locus_tag="JN691_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7241987.1"
                     /translation="DYEATLQEEKRVLVVDIGGGTTDCSLLLMGPQWRSRLDREASLL
                     GHSGCRIGGNDLDIALAFKNLMPLLGMGGETEKGIALPILPWWNAVAINDVPAQSDFY
                     SSANGRLLNDLVRDAREPEKVALLQKVWRQRLSYRLVRSAEECKIALSSVAETRASLP
                     FISNELATLISQRGLESALSQPLARILEQVQLALDNAQEKPDVIYLTGGSARSPLIKK
                     ALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR"
     gene            complement(770..2710)
                     /gene="yegI"
                     /locus_tag="JN691_000459"
     CDS             complement(770..2710)
                     /gene="yegI"
                     /locus_tag="JN691_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HAY7241988.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKLALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALWSFGIETAAGVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKMAAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(2707..3468)
                     /locus_tag="JN691_000460"
     CDS             complement(2707..3468)
                     /locus_tag="JN691_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HAY7241989.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLTLNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(3465..4124)
                     /locus_tag="JN691_000461"
                     /pseudo
     CDS             complement(3465..4124)
                     /locus_tag="JN691_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            complement(4329..4748)
                     /locus_tag="JN691_000462"
                     /pseudo
     CDS             complement(4329..4748)
                     /locus_tag="JN691_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5066..5707
                     /gene="udk"
                     /locus_tag="JN691_000463"
     CDS             5066..5707
                     /gene="udk"
                     /locus_tag="JN691_000463"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAY7241990.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5799..6380
                     /gene="dcd"
                     /locus_tag="JN691_000464"
     CDS             5799..6380
                     /gene="dcd"
                     /locus_tag="JN691_000464"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAY7241991.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            6402..8255
                     /gene="asmA"
                     /locus_tag="JN691_000465"
     CDS             6402..8255
                     /gene="asmA"
                     /locus_tag="JN691_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAY7241992.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(8707..10290)
                     /locus_tag="JN691_000466"
     CDS             complement(8707..10290)
                     /locus_tag="JN691_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY7241993.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFSFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            10949..12088
                     /locus_tag="JN691_000467"
     CDS             10949..12088
                     /locus_tag="JN691_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY7241994.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            12094..12537
                     /gene="wzb"
                     /locus_tag="JN691_000468"
     CDS             12094..12537
                     /gene="wzb"
                     /locus_tag="JN691_000468"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HAY7241995.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            12540..14702
                     /gene="wzc"
                     /locus_tag="JN691_000469"
     CDS             12540..14702
                     /gene="wzc"
                     /locus_tag="JN691_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HAY7241996.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHTRPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGGSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            14794..15633
                     /gene="wcaA"
                     /locus_tag="JN691_000470"
     CDS             14794..15633
                     /gene="wcaA"
                     /locus_tag="JN691_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HAY7241997.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEETTQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            15636..16124
                     /gene="wcaB"
                     /locus_tag="JN691_000471"
     CDS             15636..16124
                     /gene="wcaB"
                     /locus_tag="JN691_000471"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HAY7241998.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            16121..17338
                     /gene="wcaC"
                     /locus_tag="JN691_000472"
     CDS             16121..17338
                     /gene="wcaC"
                     /locus_tag="JN691_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HAY7241999.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            17313..18530
                     /gene="wcaD"
                     /locus_tag="JN691_000473"
     CDS             17313..18530
                     /gene="wcaD"
                     /locus_tag="JN691_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HAY7242000.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEVIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFSDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            18541..19287
                     /gene="wcaE"
                     /locus_tag="JN691_000474"
     CDS             18541..19287
                     /gene="wcaE"
                     /locus_tag="JN691_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HAY7242001.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLVHLAQADDISFEWIVVDGGSN
                     DATREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            19303..19851
                     /gene="wcaF"
                     /locus_tag="JN691_000475"
     CDS             19303..19851
                     /gene="wcaF"
                     /locus_tag="JN691_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAY7242002.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            19877..20998
                     /gene="gmd"
                     /locus_tag="JN691_000476"
     CDS             19877..20998
                     /gene="gmd"
                     /locus_tag="JN691_000476"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAY7242003.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            21001..21969
                     /gene="fcl"
                     /locus_tag="JN691_000477"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             21001..21969
                     /gene="fcl"
                     /locus_tag="JN691_000477"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAY7242004.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPASLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            21969..22448
                     /locus_tag="JN691_000478"
     CDS             21969..22448
                     /locus_tag="JN691_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAY7242005.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            22445..23668
                     /gene="wcaI"
                     /locus_tag="JN691_000479"
     CDS             22445..23668
                     /gene="wcaI"
                     /locus_tag="JN691_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HAY7242006.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            23671..25107
                     /gene="cpsB"
                     /locus_tag="JN691_000480"
     CDS             23671..25107
                     /gene="cpsB"
                     /locus_tag="JN691_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HAY7242007.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLCQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            25300..26670
                     /gene="cpsG"
                     /locus_tag="JN691_000481"
     CDS             25300..26670
                     /gene="cpsG"
                     /locus_tag="JN691_000481"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAY7242008.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            26725..28119
                     /gene="wcaJ"
                     /locus_tag="JN691_000482"
     CDS             26725..28119
                     /gene="wcaJ"
                     /locus_tag="JN691_000482"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAY7242009.1"
                     /translation="MINLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            28121..29599
                     /gene="wzxC"
                     /locus_tag="JN691_000483"
     CDS             28121..29599
                     /gene="wzxC"
                     /locus_tag="JN691_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HAY7242010.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIIHYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            29779..31059
                     /gene="wcaK"
                     /locus_tag="JN691_000484"
     CDS             29779..31059
                     /gene="wcaK"
                     /locus_tag="JN691_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HAY7242011.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            31056..32276
                     /gene="wcaL"
                     /locus_tag="JN691_000485"
     CDS             31056..32276
                     /gene="wcaL"
                     /locus_tag="JN691_000485"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HAY7242012.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            32287..33681
                     /gene="wcaM"
                     /locus_tag="JN691_000486"
     CDS             32287..33681
                     /gene="wcaM"
                     /locus_tag="JN691_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HAY7242013.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            33856..34749
                     /gene="galF"
                     /locus_tag="JN691_000487"
     CDS             33856..34749
                     /gene="galF"
                     /locus_tag="JN691_000487"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HAY7242014.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            35122..36207
                     /gene="rfbB"
                     /locus_tag="JN691_000488"
     CDS             35122..36207
                     /gene="rfbB"
                     /locus_tag="JN691_000488"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY7242015.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHAGICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            36207..37106
                     /gene="rfbD"
                     /locus_tag="JN691_000489"
     CDS             36207..37106
                     /gene="rfbD"
                     /locus_tag="JN691_000489"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAY7242016.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            37165..38043
                     /gene="rfbA"
                     /locus_tag="JN691_000490"
     CDS             37165..38043
                     /gene="rfbA"
                     /locus_tag="JN691_000490"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY7242017.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            38048..38593
                     /gene="rfbC"
                     /locus_tag="JN691_000491"
     CDS             38048..38593
                     /gene="rfbC"
                     /locus_tag="JN691_000491"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAY7242018.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWQVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            38609..39865
                     /gene="wzx"
                     /locus_tag="JN691_000492"
     CDS             38609..39865
                     /gene="wzx"
                     /locus_tag="JN691_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HAY7242019.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            39895..40785
                     /locus_tag="JN691_000493"
     CDS             39895..40785
                     /locus_tag="JN691_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HAY7242020.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNSTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGDGRYNA"
     gene            40814..41683
                     /locus_tag="JN691_000494"
     CDS             40814..41683
                     /locus_tag="JN691_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY7242021.1"
                     /translation="MATYNGECWIEEQLKSIIEQKDVDISIFISDDLSTDNTLNICEE
                     FQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQDDIWYKDKIKNAI
                     DCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPGCTYVIKKETLIE
                     FKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHENNQVGANISFK
                     AKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKLRRKKAD
                     KIYILLMLILNIF"
     gene            41703..42851
                     /gene="wzy"
                     /locus_tag="JN691_000495"
     CDS             41703..42851
                     /gene="wzy"
                     /locus_tag="JN691_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HAY7242022.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSAKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            42855..43229
                     /locus_tag="JN691_000496"
     CDS             42855..43229
                     /locus_tag="JN691_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HAY7242023.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            43292..44218
                     /locus_tag="JN691_000497"
                     /pseudo
     CDS             43292..44218
                     /locus_tag="JN691_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067396.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            44403..45002
                     /locus_tag="JN691_000498"
     CDS             44403..45002
                     /locus_tag="JN691_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242024.1"
                     /translation="MSLSILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFAVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYLGIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            45103..45654
                     /locus_tag="JN691_000499"
     CDS             45103..45654
                     /locus_tag="JN691_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242025.1"
                     /translation="MIAKKRNIQTFLIFSLATILGGIYWYVRSFWISGDPFSPAGGNI
                     FGHYLWNEIDLQVQTAEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKIL
                     WVFFSIVLTYVLFWFSVTQGDRYLSPIYPLSVLLVAISIASLIKDINITNYKLGRYFI
                     LVCIIFFRGDRCILNSNIFILNT"
     gene            45651..46010
                     /locus_tag="JN691_000500"
     CDS             45651..46010
                     /locus_tag="JN691_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242026.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIFISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            46119..47525
                     /gene="gndA"
                     /locus_tag="JN691_000501"
     CDS             46119..47525
                     /gene="gndA"
                     /locus_tag="JN691_000501"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAY7242027.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            47775..48941
                     /gene="ugd"
                     /locus_tag="JN691_000502"
     CDS             47775..48941
                     /gene="ugd"
                     /locus_tag="JN691_000502"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HAY7242028.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            49087..50064
                     /gene="wzzB"
                     /locus_tag="JN691_000503"
     CDS             49087..50064
                     /gene="wzzB"
                     /locus_tag="JN691_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAY7242029.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(50250..50861)
                     /locus_tag="JN691_000504"
     CDS             complement(50250..50861)
                     /locus_tag="JN691_000504"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAY7242030.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANSIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(50855..51631)
                     /gene="hisF"
                     /locus_tag="JN691_000505"
     CDS             complement(50855..51631)
                     /gene="hisF"
                     /locus_tag="JN691_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAY7242031.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            complement(51613..52350)
                     /gene="hisA"
                     /locus_tag="JN691_000506"
     CDS             complement(51613..52350)
                     /gene="hisA"
                     /locus_tag="JN691_000506"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAY7242032.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            complement(52350..52940)
                     /gene="hisH"
                     /locus_tag="JN691_000507"
     CDS             complement(52350..52940)
                     /gene="hisH"
                     /locus_tag="JN691_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAY7242033.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRHSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            complement(52940..54007)
                     /gene="hisB"
                     /locus_tag="JN691_000508"
     CDS             complement(52940..54007)
                     /gene="hisB"
                     /locus_tag="JN691_000508"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAY7242034.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            complement(54007..55077)
                     /gene="hisC"
                     /locus_tag="JN691_000509"
     CDS             complement(54007..55077)
                     /gene="hisC"
                     /locus_tag="JN691_000509"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAY7242035.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRPLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            complement(55074..56378)
                     /gene="hisD"
                     /locus_tag="JN691_000510"
     CDS             complement(55074..56378)
                     /gene="hisD"
                     /locus_tag="JN691_000510"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAY7242036.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLIRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYVAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            complement(56384..57283)
                     /gene="hisG"
                     /locus_tag="JN691_000511"
     CDS             complement(56384..57283)
                     /gene="hisG"
                     /locus_tag="JN691_000511"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAY7242037.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            complement(57429..57479)
                     /gene="hisL"
                     /locus_tag="JN691_000512"
     CDS             complement(57429..57479)
                     /gene="hisL"
                     /locus_tag="JN691_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAY7242038.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            57762..58013
                     /gene="yefM"
                     /locus_tag="JN691_000513"
     CDS             57762..58013
                     /gene="yefM"
                     /locus_tag="JN691_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAY7242039.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            58010..58264
                     /gene="yoeB"
                     /locus_tag="JN691_000514"
     CDS             58010..58264
                     /gene="yoeB"
                     /locus_tag="JN691_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HAY7242040.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            58347..59171
                     /locus_tag="JN691_000515"
     CDS             58347..59171
                     /locus_tag="JN691_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7242041.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHT
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGRIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            59217..60146
                     /locus_tag="JN691_000516"
     CDS             59217..60146
                     /locus_tag="JN691_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7242042.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            60361..60423
                     /gene="yoeI"
                     /locus_tag="JN691_000517"
     CDS             60361..60423
                     /gene="yoeI"
                     /locus_tag="JN691_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAY7242043.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            60413..61771
                     /gene="plaP"
                     /locus_tag="JN691_000518"
     CDS             60413..61771
                     /gene="plaP"
                     /locus_tag="JN691_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAY7242044.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIVLVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWICEKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
BASE COUNT        15753 a        14527 c        15721 g        15884 t
ORIGIN      
        1 tggattacga agccaccttg caggaagaaa aacgggtgct ggtggtggat atcggcggtg
       61 gtacgactga ctgttcattg ctgctgatgg ggccgcagtg gcgttcgcgt ctcgatcgtg
      121 aagccagtct gctgggtcac agtggttgtc gtattggcgg taacgatctg gatatcgccc
      181 tggcgtttaa aaacctgatg ccactgctgg gtatgggtgg cgaaaccgaa aaaggcatcg
      241 ccctgccgat cctgccgtgg tggaatgcgg ttgccatcaa cgacgttcct gcacagagtg
      301 atttctacag tagtgccaac ggtcgtctgc ttaacgatct ggtacgcgat gcccgcgaac
      361 cggaaaaagt ggccctgtta cagaaagtct ggcgtcagcg tttaagctat cgcctggtgc
      421 gtagcgcaga agagtgcaaa attgctcttt caagcgtagc ggaaacccgc gcctcactgc
      481 cgtttatcag caatgaactg gctacgctga ttagccagcg agggctggaa agcgccctca
      541 gccagccact ggcgcggatt ctggaacagg tgcaactggc cctggataac gcccaggaaa
      601 aaccggacgt tatctacctg accggcggta gcgcccgttc tccgctgatt aaaaaagcgc
      661 tggcagaaca gttgccgggc attccgattg caggcggcga tgactttggc tccgtcaccg
      721 ccgggctggc acgctgggcg gaagtggtat ttcgttaaaa gaaaatggct cagcagcggc
      781 ttaaatcagc ctgcgcctga gccagttttt ctgccgcctg acgtaacggt tccatcaacg
      841 gcatggtccg tgctggagca tgaaggcgga atcgctgcaa ctctgtcgcg ccgacagtca
      901 acgtcgattc cagccgatgt cgtttggcgg ccattttcgc cattaccgcc tgtctgtctg
      961 ctggcgttac cgcttcgttc ggcctgaaca caaaacggcg ttcacagctc gctttccagt
     1021 cgatgaccgc ctgggtcaga tgatcaccaa accctttaac ttgcttaacg ccacgacggg
     1081 taacacctgc tgctgtttca ataccaaaag accataacgc cgctttacgc gcagggccaa
     1141 cgccgggaat agaggcaaca tcaataaaaa atccctccag aaacttctgc ttctgccgtt
     1201 cccttgcagt gtcatgaagt gctgccagag cgagtttctc ttcttccggt aacccgagaa
     1261 tttcgtcctt cattttttcg agcatcgtcc gtttggcgat aaaaccttcc aggccgccta
     1321 cctgctggat ctggcggact aaatggtcat agtccatttt cgcacgatta aatgcctcac
     1381 ggcgttgctg aacttctgct ttgtacgctt tgcttgtcag actgccaata atccagatag
     1441 ccgccagcac aggaaccaaa ataatataac gcggttctgc ctgaaggccg caaagcagca
     1501 gcgataatgc tgacagtgcg atctcaagca gaatgatgta ttcgcgccgt aacaggccta
     1561 aaggaagcgg cctgccagtc ggttggaaat gatcgggtaa tggcagttgc aatgctggcg
     1621 gtgctactga cgccatcacc atcgcccaga cttttgccag cacaaaatcc ccgccggtgg
     1681 taatgacctc ttcgccgaga tcaataaaat agataacgcc ttgattatcc agcgtacacc
     1741 acgggcagtc ggtcaaatga gcgggataaa catgcattgc cgaaacggga catttcttta
     1801 actgttggcg cagagaatca agtgccgcta cccacgcttt agccgtcgga cgcccggttg
     1861 ccacgccact ttccgtgaat gcctgctgaa acatggcttc aacatcactc ggtaacatcg
     1921 atagcggaat cgatcgtggc ggcggtttta aaccacgtcg ctgattatct gacgcgtagg
     1981 cataacggaa atgggtaata tccgtctcca gcgcattacc cgcatcagag ataagcggca
     2041 caccagaata aggatgccgg ccaccaaaca agacgtgaaa aatcagcaac gcaaggccaa
     2101 aattatcgtg attttctgtg cgttcaaagc cgacaaatga cgacaaggtt tgtagctctg
     2161 gcggcgtaaa atgcgacacg ccgacttcgc ataaatgcag tgtgccattg gcgttaatct
     2221 gaaaggagtc gctatcaatc aacaccactt tgctgtcgcg acctaccata aaactgttct
     2281 ggtttacgtc acccacgaca tgcccgtgct cgtgaaccgt agcaaaagat gaagcaatat
     2341 tgcgcgcaac atagagtaga aaatcccacg cacaatgagg gtaacgttga cgacgatgtg
     2401 ccgggctata gatcatatga atcggttctt taccagaaac ttttggcatc ataaaaccga
     2461 taacttttcc gcctcgtcca ccgtgaagcg ttgcctgcgg ccaggcgaca taattcaaca
     2521 attgcgcgtc agctgtcgca gccataaagg caagtttgtc ctgttttaag gcgggtggcg
     2581 gcgtgtgata aatcttggcg acgctatcga caaactcatt gatatcataa accgcgcctt
     2641 caccgccttt gcccagttca cggcctggcg taacgcactc accagtagca gtatataaag
     2701 tgggtttcat ggtaaccaca gcgccagtgc caacgtttta tcatcatccg tacgctcgtt
     2761 taccgccggg ctggacaaaa actgctttaa caattcaggt aataaatcga gttgttcctg
     2821 cgttgctgac gccagtccat tgaaaaacgg ggtaaagaaa ggcacatgcg gagaattatc
     2881 caacatattt aacgtcagtc gctggatacc atccgtaaat gctgcaactt tatgcacacg
     2941 ctcagtgctg gtgaaagttt ccagtctgga aacggcatct tcatcggtaa tgaagtgcgt
     3001 catattggca tattcaccca ccatcggcgt aagcggcaat tgcagaccgt gaccaaaatc
     3061 caccaccacg ccgccatcgc caatctgcat aatcaaggtg ccattagccg atgaaatcaa
     3121 tcccagaaac gtgcaggcaa aatcgcgaac tgctaattct ttggcttccg cctcggcaaa
     3181 aagtcgctga cgaacggtca gcacaatatc cgtggcgagg atatcattaa gccccaattc
     3241 cccaccctgc actttttgcg acatataagc catcgcttca ttgacggcga gcatcgcgcc
     3301 ttcgcccccc tgcgaaacgc tacctgcgcc atcagcaagg aacatcacca gtaatggctg
     3361 ctggtcattt agccaggcaa cctgcatctg gcaggcatcc tggcaaggca agtccgcgct
     3421 gatgtgcgac gttccaacag cggatgcata aaccagacgc cagctcacac tgacgtccag
     3481 ccttttggcg cttccagcac aacttccgtt cccggcgtgg agcgggaaac cgaacgcagc
     3541 gaactggata atcagctaaa cagttcacgg aattgtaatc cttgcagagg caaaggctga
     3601 cgaacgctga tttgtgccag agtcttcata tccgccccct gaacaccgat ggaaaagaaa
     3661 gcaaaccttt tatcttcttc cccccgaaac actttgttgg ctgcagcctg ccactcatcg
     3721 gttggtgcac catcggtaat caggaaaatc cacgggcgat aatacgaaat accatttgca
     3781 cgatactcac gtttccgctc ttctaccata tccagggctt tggtaatggc cgcacccatt
     3841 ggtgtatcgc cctgggcaaa caggatgggc ggaaaaaaat tagcggcgct ggtaaagggt
     3901 tgttccacat gcaccgggcc gaacgtcaca atgccgagtt ccactctttt taaagccagc
     3961 gaatcggcaa gcagctcatc gcgaaaggta accaatccgg tgttaagttc attaatagtt
     4021 ctgccactca tcgagccaga aacatccagc agcagaatgc aggggcaacg tggttccggg
     4081 ttactggcaa aatcgctggt ggcaaacgtg atttgttcgc tcataagaaa tcctttctta
     4141 gagaattatt gaggtccggt atcataccga ctgcaataat gaaataagag caaacgaaga
     4201 aactttcaga attagtcccg gcggcgcaga aaattttgct attcgcctcg caagaacgga
     4261 gagccgtcga ttgacactaa gggcggagtg acataatttc aggagtgagg gttagggaga
     4321 ggtttccccc cccccaacag aggcggaaca atggcgctgc cgaatgccag acgcagccag
     4381 tcagccagat tttgcaaggg gttataccac ggcagcaatt tacgtagcag cactgccccg
     4441 accacggctt caacaatatt gatggtcgtc caggtcatgt tcagcgagct ggtggaaaaa
     4501 agcaggatgg atgcggcgat atttcccagc gaacagctca gcgcaattcc cggcaacatg
     4561 cgcccggcat ggcgataaaa cgccaccatc atgatggacg tcgggaacca cagtggggcg
     4621 agttgggtgc caaactgcga aagctcaagc gagacaaggg taaagataaa cgagactaaa
     4681 cctaaactga ccaggtgaag cagcgggtgg ggcagggcaa ttaatacatg ctgtgattgt
     4741 ttgcttatta ccgctttatc cgcaaggcca ggtcgccagt attcgtttct gtgtttatgc
     4801 tagtacaaaa gttttacatt ttatatgcga attgctcata aagtgacgta aaggcggata
     4861 atttgcgcaa ctgcgtataa cattttttac cttacataaa actgatcaac gtaatttgcc
     4921 accgggaatt ctttgtcaac aattaactct gagcgcgcgg caacgctatt cgactggtat
     4981 cagacggatg aaatccctat aattgccgcg tttggcgctt cgtcgccccc ttcctaacat
     5041 ccaggttaat caggtcgcta aatatatgac tgatcagtct catcagtgcg tcattatcgg
     5101 tatcgctggc gcatcggctt ccggcaagag tcttattgcc agtacccttt atcgtgaatt
     5161 acgtgagcaa gtcggtgatg aacacatcgg cgtaattccc gaagactgct attacaaaga
     5221 tcaaagccat ctgtcgatgg aagaacgcgt taagaccaac tacgaccatc ccagcgcgat
     5281 ggatcacagc ctgttgcttg agcatttaca agcgttgaaa cgcggctcgg caattgacct
     5341 gccggtttac agctatgttg aacatacgcg tatgaaagaa acggtgacgg ttgagccgaa
     5401 gaaggtcatc attctcgaag gcattttgtt gctgacggat gcgcgtttgc gtgacgaact
     5461 taacttctcc attttcgttg ataccccgct ggatatctgc ctgatgcgcc gcatcaagcg
     5521 tgacgttaac gagcgtgggc gttcaatgga ttccgtgatg gcgcaatatc aaaaaaccgt
     5581 gcgcccgatg ttcctgcaat tcattgagcc ttctaaacaa tatgcggaca ttatcgtgcc
     5641 gcgcggcggg aaaaaccgca tcgcgatcga tatattgaaa gcgaaaataa gtcagttctt
     5701 tgaataagct tgataaattg tgtaccgttc agtgataagc tggtatgccc ttgacgtaat
     5761 tggcgttaag ggagtgatgc gcgaaaggag aaaatgccat gcgtctgtgt gaccgagata
     5821 ttgaagcctg gcttgatgaa ggccgtttgt cgatcaaccc acgtccgcca gtggagcgta
     5881 ttaacggcgc gacggtggat gtacgcctgg gcaataaatt tcgtaccttc cgtggtcaca
     5941 cggcagcgtt tatcgatctg agcggtccca aagatgaagt gagcgccgcg cttgaccgcg
     6001 tgatgagcga tgagatcgtt ctcgacgagg gcgaggcgtt ctatcttcac ccaggagagc
     6061 tggcgctggc ggtgacgctg gagtcggtga cgctgccagc cgatctggtg ggctggctgg
     6121 acgggcgttc ctctttggcg cgtctggggc tgatggtgca cgtcaccgcg caccgtatcg
     6181 atccgggctg gtctggttgc attgtgctgg agttctacaa ctccggtaag ctgccgctgg
     6241 cgctgcgtcc gggcatgtta attggtgcgc tgagctttga gccgctttcc ggcccggcgg
     6301 cgcgacctta caaccgccgt gaagatgcga aatatcgcaa ccagcagggc gcggtagcca
     6361 gccgaatcga taaagactaa ttcaggccca ttgaggatac catgagacga tttctgacga
     6421 cgctgatgat actcctggtc gtgctggtgg ccgggttatc tgcgttagtg ttgctggtga
     6481 atccgaatga tttccgcgac tatatggtca agcaagttgc tgcacgtagc ggttatcaat
     6541 tgcagctcga cgggccactg cgttggcacg tctggccgca gcttagtatc ctctccgggc
     6601 gaatgtctct caccgcccag ggcgcaagcc agccactggt tcgcgccgac aacatgcgtc
     6661 tggacgtggc gcttttacca ctactgagtc atcaactgag cgttaagcag gtgatgctaa
     6721 aaggggcagt gatccaactg acgccgcaga cggaagcggt gcgcagtgaa gacgctccgg
     6781 ttgcaccgcg cgacaatacc ttgccggatc tgtcagacga tcgcggatgg tcgtttgata
     6841 tatccagtct taaggtggcg gacagcgttc tggtgttcca gcatgaagat gacgagcagg
     6901 tgacaatccg caatatccgc ctgcaaatgg aacaagatcc ccaacatcgt ggctcatttg
     6961 agttctccgg gcgggttaat cgcgatcagc gcgatctcac gatatccctt aacggtacgg
     7021 tagatgcttc tgattatccg catgatttaa cagcggctat tgaacaaatt aactggcagt
     7081 tgcagggtgc cgatttacca aaacaaggta ttcaggggca ggggagtttc cagtcccagt
     7141 ggcaggagtc acataaacgc ctttcattta accaaattag tttgaccgcc aatgacagta
     7201 cgctgagcgg gcaagcacag gtcacgctga cagagaaacc ggaatggcag ctgagactgc
     7261 aattcccgca actgaatctt gacaacctca tcccgcttaa tgaaaccgcg aatggtgaaa
     7321 acggtgccgc gcagcagggg cagagccaat caacgttgcc gcgcccggtc atttcttcgc
     7381 gtattgatga accggcctat cagggactgc aaggctttac agctgatatt ttgttgcagg
     7441 ccagtaacgt gcgctggcgc ggaatgaatt ttacagatgt tgccacgcaa atgaccaaca
     7501 agtcgggttt gctggaaatt actcaactgc agggcaagct taacggtgga aaggtttcac
     7561 tgccgggcac gctggacgcg acatcaataa atccgcggat aaacttccag ccacggctgg
     7621 agaacgttga gattggtacc attctgaagg cgtttaacta tccgatttcg ttgaccggaa
     7681 aaatgtcact ggctggtgat ttctccggtg ctgacataga tgccgacgca ttccgccaca
     7741 actggcaagg gcaggcgcat gttgagatga ccgacacacg tatggaaggg atgaacttcc
     7801 agcagatgat tcagcaagcg gtagagcgta atggcggtga tgtgaaggcc gctgaaaact
     7861 tcgataacgt gacgcggctt gaccgcttta ccaccgattt gacgttgaag gatggcgtcg
     7921 tgacgttaaa cgacatgcaa ggtcaatcgc ctgtgctggc gctgacaggg gaaggcatgt
     7981 tgaatctggc agatcaaacc tgcgacaccc agtttgatat tcgggtcgtg ggtggctgga
     8041 acggggaaag caaactgatt gatttcctca aagaaacgcc agtaccgctg cgggtttatg
     8101 gcaactggca gcaactcaat tacagcctgc aagtggatca gttactgcgc aaacatctac
     8161 aggacgaagc gaaacgtcgc ctgaatgact gggccgagcg gaataaagat tcccgcaatg
     8221 gcaaagatgt gaagaagttg ctggagaaga tgtaggttac caggtgacct gtcctgtagg
     8281 cctgataaga cgcgtaaagc gtcgcatcag gcgttcgcgg cggcactatt atattctgaa
     8341 tatgtaagat cgaaggctgt tccgttcact tgatgttcct cgctactatg gaacgctcaa
     8401 gccggtgaac gtgccagggt ggctcaatcg ccaccaccct ggcaacccgg gctcccggcg
     8461 gtaaatcgcc gctggcgcgg ttccctcatc ttattccttt cggctatcgg gtacgggcgc
     8521 gaggtaacat ccctgtaaaa cgcgccctca gcccacatcc atgtgggctg ccccagccta
     8581 caggaaacga ttcggcgatt tacagccgga cccacaccac cgctgtgatg tccgtttacc
     8641 acagacaacg cttcattgct gaagttataa ggcgttacgc agcattgggt aaaatggccg
     8701 ataccatcac acctcgtact ccatctcgcc atccttacgc agcggtataa tctgcacctt
     8761 ctgcacgcga tggctttcca cctgcaacgt tttaagcaaa taatccccca cctgaacttc
     8821 ttcgccaggt tttggaatac gctgcaaata ctccatcaac agcccggcaa tggtgtgata
     8881 ttcacgtttt tcatccagcg gcagcggcac atattgcacc agatcctcca gcggcatatg
     8941 accattcgcc gtccaggaac cgtccgcatt cttctgaata tcatggcggg cgtcgatctc
     9001 ttccacttcg ttcggtaagt taccggcaat ggtttcagtg acgtcactta atgtcacaat
     9061 cccttccacc gagccaaact catccaccac aaaagcaaag tgcgtgcggg cattacggaa
     9121 ctgctccagg gctggtaaca acggcaaggt ttccgggaac accagcggct ggcgaatcaa
     9181 cacccgcagg ttgagcggtt cgccgcgcag tgactgttgt agcaggtcga taacgtgaac
     9241 aacaccgagc aaatcttctg cgtcatcacc gtcggtaacc accagccgcg tatgctgatt
     9301 tcgctccagc aactggcgga tttcttcttc cggcgcgttg agatcgatat gctcaatatc
     9361 gtggcgcgac gtcataatgc tgctgacggt acgctggtta agattaagta cccgctcaat
     9421 catccgccgt tcctgcggat taaagatctg ctggtttcca tgatccacca gcatggacgc
     9481 ggtttcggcg tccagttccg catcttcttt ttgcccgcta agcaggcgca ttaccgcttc
     9541 ggtagtccgc tggcgtagcg tctgattagc ggaaagaaaa cgtcgccggt taaagatagc
     9601 caactgattg agtgcctcga tcatcactga gaaaccaatg gcagcgtaca ggtagccttt
     9661 cggaatgacg aagctgaaac cttctgccac caggctaaag ccaatcatca acaggaagct
     9721 taagcagaga ataacgatgg tcgggtgact gttaacaaat tgcgttaacg gcttgctggc
     9781 catcaacatc aggctgatag cgataaccac ggcggccatc atgaccagta aatggtcgac
     9841 catcccgacg gcggtaatca ctgagtcgag tgagaaaatg gcgtccagta ccacaatttg
     9901 cgtcaccacg ccccagaact tcgcaccttt gcgttgtgtg ggattattgc tgtctttccc
     9961 ttccagccgt tcgttcagct ccatcgtggc tttgaacaac aggaagaaac caccaaacag
    10021 catgattaag tcgcgggcgc taaaggtaaa cgagcggaag ctgaacagcg gttgagtcag
    10081 ggtgaccagc caggagattg acgccagcag taacaggcgc attaacatcg ccagcagcag
    10141 cccggtaacc cgtgcgcggt cgcgctgctt cggcggtagt ttttcggcga ggatggcaat
    10201 aaagaccagg ttatcaatgc cgaggaccag ttcgatcaca atcagcgtga ttaacccggc
    10261 ccagatagac ggatcggcaa tccattccat agtaagtaca aaacctattc gttgtatgac
    10321 aaatgtcaca attcgatcat ggataaatcg gggcaaaaat gcaatgttgc agcactattt
    10381 atctcatatt atttgagggt aataacctca cattatccct gaattaaaag cggtaataat
    10441 aaagcattat ttaagaaata tcgcatatgg agacgcaata ttaaatattg tcaacctaaa
    10501 gaaactccta aaaaccatat tgaatgacac ttaatataat tcttaaaaat agccaattgc
    10561 cgaattgtta ccttgcctgc tattccgtta gctgtaacac ttcctcctgc attattggag
    10621 agccaatatt caattaccat aatgtcacct tatattcgga agttaatttc cgcaataacc
    10681 ctttctcatc aacgattgta tgcagagagg gtttgaaaaa ttaagctcaa tcagtgtatt
    10741 ggtagctaaa aagccagggg cggtagcgtg tctggatgcc tgaaagacca gtctgaatta
    10801 ttctgtcaat agcctgcgga tgaaatcaat ttttttagga ctgatgccag ttaaattttt
    10861 attcaattaa ctgcaaggta aatgtgcatg gcgacattgc tattaatagt gcacaggata
    10921 attactctgc caaagtgata aataaacaat gatgaaatcc aaaatgaaat tgatgccatt
    10981 attggtgtca gtaaccttga taagcggttg cacagtactt ccgggcagca atatgtcgac
    11041 gatgggcaaa gacgtcatca aacagcagga cgctgatttc gatctcgaca aaatggtgaa
    11101 tgtttatccg ctgaccccgc gcctgattga ccaattacgc ccacgcccga atgtagcgcg
    11161 ccccaatatg acgctggaaa gcgagatcgc gaattaccag tatcgcgtcg ggccggggga
    11221 tgttcttaat gtcaccgtct gggatcaccc ggaactcacc acgccagctg gtcagtaccg
    11281 cagctccagc gacaccggca actgggtaca gcctgacggc actatgtttt acccgtatat
    11341 cggcaaggtt cacgtagtcg ggaaaacgct cgctgaaatc cgcagtgata ttaccgggcg
    11401 cttagcgacg tacatcgctg acccgcaggt ggacgttaat atcgccgcct tccgctcaca
    11461 aaaggcctat atctcaggtc aggtgaataa atccggtcaa caggcgatca ccaacgtacc
    11521 actgaccatt ctcgacgcca tcaacgccgc aggtggcctg accgacaccg ctgactggcg
    11581 caacgtggtg ctaacacaca atggtcgtga agaacgcatt tctttacagg cgctgatgca
    11641 aaacggcgac ctcaatcaga accgactgct ttatcccggc gatattctct acgtgccgcg
    11701 taatgatgat ctgaaagtat ttgtgatggg tgaagtgaag aaacagagca ccctgaaaat
    11761 ggactttagc ggcatgaccc tgactgaagc tctgggcaat gccgaaggta tcgacatgac
    11821 cacctccaac gccagcggca tctttgtcat tcgtccgctg aaaggcgagg gcgggcgtaa
    11881 cggcaagatt gccaatatct accagctgga tatgtccgat gcgacgtcgc tggtgatggc
    11941 gacagaattc cgcctgcaac cttatgacgt ggtgtatgtc accaccgccc cggtttcccg
    12001 ctggaaccgt ctgatcaatc agttgctgcc aactattagc ggtgtccgtt acatgacgga
    12061 tacagccagc gacattcata actggtaatc gtcatgttta acaacatctt agttgtctgt
    12121 gtcggcaata tttgccgttc cccgatggcg gaacgcttac tgcaacgtta tcacccggaa
    12181 ctgaaagtgg agtccgctgg actcggcgcg ctggtcggta agggcgctga tcctaccgct
    12241 atcagcgtcg ccgcagaaca tcaactgtct ctggaaggcc actgtgcacg acaaatcagc
    12301 cgccgcctgt gtcgcaacta tgacctgatt ttgaccatgg ataagcgcca tatcgaacgc
    12361 ttatgcgaga tggcaccgga gatgcgcggc aaagtgatgc tgtttggtca ctgggataac
    12421 gaatgtgaaa tccccgatcc gtatcgcaaa agccgggaaa cgtttgcagc ggtgtacaca
    12481 ttacttgaac ggtctgcccg ccagtgggcg caggcattga acgcagagca ggtataagaa
    12541 tgacagaaaa agtaaaacaa catgccgctc cggtaacggg cagtgatgaa atcgatattg
    12601 gtcgcctggt cggcaccgtc attgaagcgc gctggtgggt gattggcatc accgccgtat
    12661 ttgccctctg tgccgtggtt tacactttct tcgccacgcc gatttatagt gccgacgcac
    12721 tggtacaaat cgagcaaaac agcggcaatt cgctggtgca ggatatcggc tcggcattag
    12781 ccaacaaacc gcctgcatcg gacgccgaga tccagttgat tcgttcgcgt ctggtgcttg
    12841 gtaaaacggt ggacgatctc gacctcgata ttgcggtgag caaaaacacg ttcccgattt
    12901 tcggggcggg ctgggatcgc ctgatggggc gtcagaacga gacggtgaaa gtgactacct
    12961 ttaaccgccc gaaagagatg gcggatcagg tgtttacgct taatgtgctg gatgacaaaa
    13021 actacaccct gagcagtgat ggcggcttta gcgcccgtgg gcaagcgggc cagatgctga
    13081 aaaaagaagg cgtcacgctg atggttgaag ccattcacac ccgcccgggc agtgagttta
    13141 ccgtcaccaa atactccacg ttggggatga tcaaccaact gcaaaacagc ctgacggtaa
    13201 cggagaacgg caaagacgca ggcgtactga gcctgactta taccggtgaa gatcgcgaac
    13261 agatccgcga cattcttaac agcatcgctc gtaactatca ggaacaaaat attgagcgca
    13321 aatcggcgga agcgtcgaaa agcctcgctt tcctcgcgca acagttaccg gaagtacgta
    13381 gccgcctcga tgttgccgaa aacaaactga atgccttccg tcaggataaa gattctgttg
    13441 atctgccgct tgaagcgaaa gcggtgctcg attcgatggt gaacatcgac gcgcagttga
    13501 acgaactgac ctttaaagag gcggaaatct ccaagctgta caccaaagtt cacccggcgt
    13561 accgcacgct gctggagaaa cgtcaggcgc tggaagaaga gaaagccaaa cttaacggtc
    13621 gcgtaacggc gatgccgaaa acccagcagg aaattgtccg tctgacccgc gatgtcgagt
    13681 ctggtcagca ggtctatatg caactgctga ataaagagca ggagctgaaa atcaccgaag
    13741 ccagcaccgt cggcgatgtg cgcattattg acccggcaat cacccagcct ggcgtgctaa
    13801 aaccgaagaa agggctgatt atcctcggcg cgattatcct tggcctgatg ctctctatcg
    13861 tgggtgtgct gctgcgctcg ttgtttaatc gcggcatcga aagcccgcaa gtgctggaag
    13921 aacacggaat cagcgtctat gccagcatcc cgctgtcgga gtggcagaaa gcgcgcgata
    13981 gcgtcaaaac catcaaaggg gttaaacgct ataaacagag ccagctactg gcagtgggga
    14041 acccaaccga tctggcgatt gaagccatcc gtagtctgcg taccagtctg cacttcgcga
    14101 tgatgcaggc gcagaacaat gtgttgatga tgaccggggg tagcccgtca atcggtaaaa
    14161 cctttgtctg cgccaacctg gcggcggtga tcagccagac caataaacgc gtgttgttga
    14221 tcgactgcga tatgcgcaaa ggctacaccc acgagctgtt gggcaccaat aatgtcaatg
    14281 ggctgtcgga gattctgatt ggtcagggcg atattactac cgctgctaaa ccgacctcta
    14341 ttgccaaatt tgacctgatc ccgcgcggtc aggtgccgcc aaatccttct gaactgttaa
    14401 tgagcgaacg ctttgccgaa ctggtgaact gggcgagtaa aaactatgac ctggtgttga
    14461 ttgatacgcc gccgattctg gcagtgaccg atgcggcaat tgttggtcgt catgtcggaa
    14521 ccacgttaat ggtggcgcgt tatgcggtca acacattgaa agaagtggaa accagtctga
    14581 gccgctttga gcaaaacggt attccggtga aaggggtgat tctgaactcc atcttccgcc
    14641 gcgccagcgc gtatcaggat tatggctatt acgaatacga atataagtcg gatgcgaaat
    14701 aacgacgcct gcattcgcac cgccccgtag gccggataag gcgctccgcc gcatccggca
    14761 agcaaaccag ctcataagcc gggagaacaa cctatgaaag acaatccgct gatctcaatc
    14821 tatatgccaa cctggaaccg ccaacaactg gcgattcggg cgataaaatc ggtcctgcgc
    14881 caggactaca gcaactggga gatgatcatc gtggatgatt gctccacctc ctgggaacaa
    14941 ctgcaacagt acgtcactgc cctcaacgat ccgcgtatca cttacatcca caacgacatt
    15001 aacagcgggg cgtgcgcggt gcgtaaccag gcgattatgc tggcgcaagg ggaatacatc
    15061 accgggatcg atgatgatga tgaatggacg cccaaccgcc tgagcgtctt cctcgcccat
    15121 aaacagcaac tggtcacaca cgcctttttg tacgctaacg actacgtttg ccagggagaa
    15181 gtttattccc aaccggcgag cttaccactg tatcccaaat cgccgtactc acgccgcctg
    15241 ttctataaac gcaatatcat tggcaatcag gtctttacct gggcatggcg tttcaaagag
    15301 tgtttgttcg ataccgaact gaaagcagca caggattacg acatattcct gcggatggtg
    15361 gtggagtacg gcgaaccgtg gaaagtggaa gagacgacgc agatcctgca tatcaatcac
    15421 ggcgaaatgc agatcacctc gtcaccgaaa aaattctccg gatacttcca cttttaccgc
    15481 aagcacaaag acaaattcga ccgcgccagt aaaaaatatc agctgtttac cctctaccag
    15541 atccgcaata aacgtatgac ctggcgcacc ttgttgacgc tgctgtcggt gcgtaacggc
    15601 aaacgtctgg ctgatggtat tcgggggcgc taaagatgct ggaagatctg cgcgccaaca
    15661 gctggagttt acgcccgtgc tgcatggtac ttgcctatcg cgtcgctcat ttttgttcgg
    15721 tgtggcgcaa aaagaacgtc ctcaacaatc tgtgggcggc cccgctgctg gtgctgtatc
    15781 gcattatcac cgaatgcttt ttcggttatg aaatccaggc cgccgcgacc attggccgcc
    15841 gctttactat ccatcacggt tacgccgtag tgatcaataa aaacgtggtg gcgggggatg
    15901 atttcaccat tcgccacggc gtcactatcg gcaatcgggg tgccgataac atggcatgtc
    15961 cacacattgg caacggcgtc gaactcggtg ccaacgtcat tattcttggt gatatcacgc
    16021 ttggtaacaa cgtcaccgtg ggtgcgggca gcgtggtgct cgactctgtc ccggacaacg
    16081 cgctggtggt gggagaaaaa gcgcgagtga aggtaattaa atgaatattc tgcaatttaa
    16141 tgtgcgactg gcggaaggcg gggcagcagg tgtggcgtta gatctccacc agcgtgcgct
    16201 gcaacagggg ctggcgtcac attttgtcta cggctacggc aaaggcggca aagagagcgt
    16261 cagccatcag aactatccgc aggtcatcaa acatacgccg cggatgaccg cgatggcgaa
    16321 cattgcgttg tttcgtctgt ttaatcgcga tctgttcggc aatttcaatg agttatatcg
    16381 caccattact cgcacaccgg gtccggtggt cctgcatttt catgtgctgc acagctactg
    16441 gttgaatctt aacagcgtgg tgcgcttttg cgaaaaagtg aaaaaccata aaccggatgt
    16501 caccctggtc tggacgctgc acgaccactg gagtgtaacc ggacgctgcg cctttaccga
    16561 cggttgcgaa ggctggaaaa cgggctgcca gaaatgcccg accttaaata actatccgcc
    16621 ggtgaagatt gatcgcgcgc accagctggt ggcgggcaaa cgccagttat tccgcgagat
    16681 gctggcgctg ggctgtcagt ttatttcccc cagccagcat gtggctgacg ctttcaatag
    16741 tctgtacggg ccagggcgtt gccggattat caataatggc attgatatgg caaccgaagc
    16801 gattctggca gatctgcctc cggtgcgcga aactcagggc aagccgaaaa tcgcggtggt
    16861 ggcgcatgac ctgcgttacg acggcaaaac taaccagcaa ctggtgcgtg agatgatggc
    16921 gctgggcgac aaaatcgaac tgcatacctt tggtaagttc tcgccgttca ccgctggcaa
    16981 cgtggttaat cacggctttg aaactgacaa gcgcaagttg atgagcgcgc tcaatcagat
    17041 ggatgcgctg gtgttcagtt ctcgtgtcga taactacccg ctgattttgt gtgaggcgct
    17101 atcgattggc gtgccggtga ttgccaccca tagcgatgcg gcgcgggaag tgctgcaaaa
    17161 atccggtggt aaaaccgtca gtgaagaaga ggtgctgcaa ctggtacagt taagcaaacc
    17221 ggaaatcgcg caggcgatat ttggtaccac gctggctgag ttcagccaac gcagtcgcgc
    17281 cgcctacagt ggacaacaga tgctggagga gtatgtcaac ttctatcaga atctgtagct
    17341 acctgctgct gccactgatc tacctgctgg ttaacgtcaa aatcgcccag cttggcgaaa
    17401 gtttccccat caccatcgtc acttttttac ctgtcttgtt actgctgttt ttagaacgca
    17461 tcagcattaa aaaattgatg attgccttag ggattggcgc gggactcacg gcgtttaact
    17521 acctgtttgg tcagtcgttg gatgccggta aatacgtcac ttcaactatg ctgtttgtct
    17581 atattgtgat cattattggc atggtgtgga gtattcgttt taaaacaatt tcgccacaca
    17641 accatcgtaa gatattacgt ttcttttatc tggtggtcgg actggtggtg gcgctggcgg
    17701 cggtggagat ggcgcaaatt atcctcaccg gtggcagcag tattatggag tcgatttcga
    17761 aatatctgat ttacagcaac agctatgtgc tgaatttcat taaattcggc ggcaagcgca
    17821 cgacagcact ttatttcgaa ccggcatttt tcgctctggc attaatctca atttggctca
    17881 gcatcaaaca gtttggtatc aaaacgccta aaacagatgc tatgattctc gcagggataa
    17941 tattatccgg atcgttttca ggggttatga cctttatcct gttttatttg ctggagtggg
    18001 catttcaata tctgaataaa gaggtgatta agaaaaaatt accgttagca ttgatttctc
    18061 tggctgtatt cctggttggt gtggtaatcg cgtttcctta tatttccacc cgtctgggcg
    18121 atttaggtac ggaaggatcg tcatcgtatt atcgtattgt cggtccgctg gtgatggtcg
    18181 gttattcttt gacccatatt gacggtgtag tcagatttgg ctcactttat gaatatgttg
    18241 catcattcgg aatatttaac ggtgcggatg tcggaaaaac catagacaat ggtttgtatc
    18301 tgctgattat ttatttttcc tggttcgcgg tgtttttatc gctgtggtac atggggaaag
    18361 tgataaaaat gatgatcaac gcttttagtg ataaccgcaa ttttcgcgtg caattatatc
    18421 tttttactcc ggtatcgctg ttttttaccg gttcgatatt tagcccggaa tatgcatttt
    18481 taatcgtctg tccgtttatt ttgcgaaaag cgttaaatat tacgaggtaa gaataagaac
    18541 atgttgctta gcataatcac tgtcgcgttt cgtaacctcg aagggatagt caaaacacat
    18601 gcctcgctgg tgcatctggc gcaggcggac gatatcagct tcgaatggat tgttgtcgat
    18661 ggcggttcca acgacgctac ccgtgagtat ctggaaaatc tcaatggtat ctataaccta
    18721 cgctttgtca gcgagccaga taacggtatc tacgacgcca tgaacaaggg cattgcgatg
    18781 gcacaaggca agttcgcgtt gtttctcaac tcgggcgata tttttcatca ggatgccgca
    18841 aattttgtcc gtaagttaaa aatgcaaaaa gataacgtga tgatcactgg cgatgcgctg
    18901 ctggattttg gcgacgggca taaaattaaa cgtagcgcca aaccgggctg gtatatttat
    18961 cacagcctgc ccgccagcca tcaggcgata tttttcccgg tatccggctt gaaaaaatgg
    19021 cgttatgacc tggaatataa agtttcttct gattacgcgc tggcagctaa aatgtataaa
    19081 gccggttatg catttaaaaa actcaatggc ctggtgtctg aattttctat gggtggggta
    19141 tcaaccacca ataatatgga attgtgtgct gacgccaaaa aagtccaacg gcagatatta
    19201 catgtacctg gcttctgggc agaattatcc tggcatttac gccaacgtac gacaggtaag
    19261 actaaagcct tatataacaa agtctgaata taaggaaaac caatgcaaga tttaagcggt
    19321 ttctcggtgc cgaaagggtt ccggggcggc aacgctatta aagtgcaatt atggtgggca
    19381 gtacaggcaa caatatttgc ctggtcgcca caagtattgt atcgctggcg ggctttttta
    19441 ttacgtttat tcggagcaaa aataggaaaa aacgtagtta ttcgtccgtc agtaaaaatt
    19501 acctatccgt ggaaattaac cttaggtgat tacgcgtggg tcggcgatga cgtcaattta
    19561 tataccctcg gtgaaataac cattggcgca cattcggtga tatcgcaaaa aagttattta
    19621 tgcaccggta gccacgacca tgcaagtcaa catttcacca ttaacgccac gcctattgtg
    19681 attggcgaga aatgctggct ggcaaccgat gtctttgttg ccccaggcgt cacaatcggc
    19741 gacggcaccg tcgtgggtgc acgaagcagt gtttttaaat cgcttccggc aaatgtggtt
    19801 tgccgaggga atcccgcagt ggtgatacgc gaacgcgttg aaactgaata aattcaaaaa
    19861 tacagaggaa taagacatgt caaaagtcgc tctcatcacc ggtgtaaccg gacaagacgg
    19921 ttcttacctg gcagagtttc tgctggaaaa aggttacgag gtgcatggta ttaagcgtcg
    19981 cgcatcgtca ttcaacaccg agcgcgtgga tcacatttat caggatccgc acacctgcaa
    20041 cccgaaattc catctgcatt atggcgacct gagtgatacc tctaacctga cgcgcatttt
    20101 gcgtgaagta cagccggatg aagtgtacaa cctgggcgca atgagccacg ttgcggtctc
    20161 ttttgagtca ccagaatata ccgctgacgt cgacgcgatg ggtacgctgc gcctgctgga
    20221 ggcgatccgc ttcctcggtc tggaaaagaa aactcgtttc tatcaggctt ccacctctga
    20281 actgtatggt ctggtgcagg aaattccgca gaaagaaacc acgccgttct acccgcgatc
    20341 tccgtatgcg gtcgccaaac tgtacgccta ctggatcacc gttaactacc gcgaatccta
    20401 cggcatgtac gcttgtaacg gaattctctt caaccatgaa tccccgcgcc gcggcgaaac
    20461 cttcgttacc cgcaaaatca cccgcgcaat cgccaacatc gcccaggggc tggagtcgtg
    20521 cctgtacctc ggcaatatgg attccctgcg tgactggggc cacgccaaag actacgtaaa
    20581 aatgcagtgg atgatgctgc aacaggaaca gccggaagat ttcgttatcg cgaccggcgt
    20641 tcagtactcc gtacgtcagt tcgtggaaat ggcggcagca cagctgggca tcaaactgcg
    20701 ctttgaaggc acgggtgttg aagagaaggg cattgtggtt tccgtcaccg ggcatgacgc
    20761 gccgggcgtt aaaccgggtg atgtgattat cgccgttgat ccgcgttact tccgtccggc
    20821 agaagttgaa acgctgctcg gcgacccgac caaagcgcac gaaaaactgg gctggaaacc
    20881 ggaaatcacc ctcagagaga tggtgtctga aatggtggct aatgacctcg aagcggcgaa
    20941 aaaacactct ctgctgaaat ctcacggcta cgacgtggcg atcgcgctgg agtcataagc
    21001 atgagtaaac aacgcatttt tatcgctggc cattgtggga tggtcggttc tgccatcaag
    21061 cggcagctcg aacagcgcgg tgatgtggag ctggtattac gtacccgcga tgaactgaac
    21121 ctgctggaca gccgcgccgt gcatgatttc tttgccagcg aacgcattga ccaggtctat
    21181 ctggcggcgg cgaaagtggg cggcattgtt gccaacaaca cctatccggc ggatttcatc
    21241 taccagaaca tgatgattga gagcaacatc attcacgccg cgcatcagaa cgacgtgaac
    21301 aaactgatgt ttctcggatc gtcctgcatc tacccgaaac tggcaaaaca gccgatggca
    21361 gaaagcgagt tattgcaggg cacgctggag ccgaccaacg agccttacgc cattgccaaa
    21421 atcgccggga tcaaactgtg cgaatcttac aaccgccagt atggacgcga ttaccgctct
    21481 gtcatgccga ccaacctgta cgggccgcat gacaacttcc acccgagtaa ctcccatgtg
    21541 ataccagcat cgctgcgtcg cttccacgag gcgacggcac agaatgcgcc ggacgtggtg
    21601 gtatggggca gcggtacacc gatgcgtgaa ttcctgcacg tcgatgatat ggcggcggcg
    21661 agcattcatg tcatggagct ggcgcatgaa gtctggctgg agaataccca accgatgctg
    21721 tcgcacatta acgtcggcac gggcgttgac tgcactatcc gcgagctggc gcaaaccatc
    21781 gccaaagtgg tgggttacaa aggtcgggtg gtttttgatg ccagcaaacc ggatggtacg
    21841 ccgcgcaaac tgctggatgt gacgcgcctg catcagcttg gctggtatca cgaaatctca
    21901 ctggaagcgg ggcttgccag cacttaccag tggttccttg agaatcaaga ccgctttcgg
    21961 ggggaatgat gtttttacgt caggaagact ttgccactgt agtgcgctcc acaccgcttg
    22021 tctctctcga ctttattgtc gagaacagtc gcggcgagtt tctgcttggc aaaagaacca
    22081 accgcccggc gcagggttac tggtttgtgc cgggagggcg cgtgcagaaa gacgaaacgc
    22141 tggaagccgc atttgagcgg ctgacgatgg cggaactggg gctgcgtctg ccgataacag
    22201 caggccagtt ttacggtgtc tggcagcatt tttatgacga taacttctcc ggcacggatt
    22261 tcaccactca ctatgtggtg ctcggttttc gcttcagagt agcggaagaa gagctgttac
    22321 tgccggatga gcagcatgac gattaccgct ggctgacgcc ggacgcgctg ctcgccagta
    22381 acgatgttca cgctaacagc cgcgcctatt ttctcgctga gaagcgtgcc ggagtacccg
    22441 gattatgaaa atactggtct acggcattaa ctactcgccg gagttaaccg gcatcggcaa
    22501 atacaccggc gagatggtgg aatggctggc ggcacaaggt catgaggtgc gggtcattac
    22561 cgcaccgcct tactacccgc aatggcaggt gggtgagaac tattccgcct ggcgctacaa
    22621 acgagaagag ggggccgcca cggtgtggcg ctgcccgctg tacgtgccaa aacagccgag
    22681 caccctgaaa cgcttgttgc atctcggcag ttttgccgtc agcagtttct ttccactgat
    22741 ggcgcaacgt cgctggaagc cggatcgcat tattggcgta gtgccaacgc tgttttgcac
    22801 gccgggaatg cgcctgctgg cgaagctctc tggtgcgcgt accgtgctgc atattcagga
    22861 ttacgaagtg gacgccatgc tggggctggg ccttgccgga aaaggcaaag gcggcaaagt
    22921 ggcacagctg gcaacggcgt tcgaacgtag cggactgcat aacgtcgata acgtttccac
    22981 gatttcgcgt tcgatgatga ataaagccat cgaaaaaggc gtggcggcgg ataacgtcat
    23041 cttcttcccc aactggtcgg aaattgcccg ttttcagcat gttgcagacg ccgatgttga
    23101 tgcccttcgt aaccagcttg gcctgccgga taacaaaaaa atcattcttt actccggcaa
    23161 tattggtgaa aagcaggggc tggaaaacgt tattgaagct gccgatctcc tgcgcgatga
    23221 accgctgatt tttgccattg tcgggcaggg cggcggcaaa gcgcggctgg aaaaaatggc
    23281 gcagcagcgt ggactgcgca acatgcaatt tttcccgctg caatcgtatg acgctttacc
    23341 cgcactgctg aagatgggcg attgccatct ggtggtgcaa aaacgcggcg cggcagatgc
    23401 cgtattgccg tcgaaactga ccaatattct ggcggtaggc ggtaacgcgg tgattaccgc
    23461 agaggcccac acagaactgg ggcagctttg cgaaaccttt ccgggtattg cggtttgcgt
    23521 agaaccggaa tcggtcgagg cgctggtggc ggggattcgt caggcgctcc tgctgcccaa
    23581 acataacacg gtggcacgtg aatatgccga acgcacgctc gataaagaga acgtgttacg
    23641 tcaatttata aatgatattc ggggataatt atggcgcagt cgaaactcta tccagttgtg
    23701 atggcaggtg gctccggtag ccgcttatgg ccgctttccc gcgtacttta ccccaagcag
    23761 tttttatgcc tgaaaggcga tctcaccatg ctgcaaacca ccatctgccg cctgaacggc
    23821 gtggagtgcg aaagcccggt ggtgatttgc aatgagcagc accgctttat tgtcgcggaa
    23881 cagctgtgtc aactgaacaa actcaccgag aacattattc tcgaaccggc agggcgtaac
    23941 actgcacctg ccattgcact ggcggcgctg gcggcaaaac gtcatagccc ggagagcgac
    24001 ccgttaatgc tggtattggc ggcggatcat gtgattgccg atgaagacgc gttccgtgcc
    24061 gccgtgcgta atgccatgcc ttatgccgaa gcgggcaagc tggtgacctt tggcattgtg
    24121 ccggatctac cagaaaccgg ttatggctat attcgtcgcg gtgaagtgtc tgcgggtgag
    24181 caggatacgg tggcctttga agtggcgcag tttgtcgaaa aaccgaatct ggaaaccgcc
    24241 caggcctatg tagcaagcgg cgaatattac tggaacagcg gcatgttcct gttccgcgcc
    24301 ggacgctatc tcgaagaact gaaaaaatat cgcccggata tcctcgatgc ctgcgaaaaa
    24361 gcgatgagcg ccgtcgatcc ggatctcgat tttattcgtg tggatgaaga agcgtttctc
    24421 gcctgcccgg aagagtcggt ggattacgcg gtcatggaac gtacggcaga tgctgttgtg
    24481 gtgccgatgg atgcgggctg gagcgatgtt ggttcctggt cttcattatg ggagatcagc
    24541 gcccacaccg ccgagggcaa cgtttgccac ggcgatgtga ttaatcacaa aactgaaaac
    24601 agctatgtgt atgccgaatc aggcctggtc accaccgtcg gggtgaaaga tctggtagtg
    24661 gtgcagacca aagatgcggt gctgattgcc gaccgtaacg cggtacagga tgtgaaaaaa
    24721 gtggtcgagc agatcaaagc cgatggtcgc catgagcatc gggtgcatcg cgaagtgtat
    24781 cgtccgtggg gcaaatatga ctctatcgac gcgggcgacc gctaccaggt gaaacgcatc
    24841 accgtgaaac cgggcgaggg tttgtcggta cagatgcacc atcaccgcgc ggaacactgg
    24901 gtagtggtcg cgggaacggc aaaagtcacc attgatggtg atatcaaact gcttggtgaa
    24961 aacgagtcca tttatattcc gctgggggcg acgcactgcc tggaaaaccc ggggaaaatt
    25021 ccgctcgatt taattgaagt gcgctccggc tcttatctcg aagaggatga tgtggtgcgc
    25081 ttcgcggatc gctacggacg ggtgtaaacg tcgcatcagg cgctgtttgt cggatgcggc
    25141 gtgaacgcct tatccgacct acggttcggt tttgtaggcc tgataagacg cggcagcgtc
    25201 gcatcaggca atgaatgcga aaccgcggtg taaataacga caaaaataaa attggccgtt
    25261 tcggtcaggg ccaactattg cctgaaaaag ggtaacgaca tgaaaaaatt aacctgcttt
    25321 aaagcctatg atattcgcgg gaaattaggc gaagaactga atgaagatat cgcctggcgc
    25381 attggtcgcg cctatggcga atttctcaaa ccgaaaacca ttgtgttagg cggtgatgtc
    25441 cgtctcacca gcgaaacctt aaaactggcg ctggcaaaag gtttacagga tgcgggcgtc
    25501 gatgtgctgg atatcggtat gtccggcacc gaagagatct atttcgccac gttccatctc
    25561 ggcgtggatg gcggcattga agttactgcc agccataatc cgatggatta caacggcatg
    25621 aagctggtgc gcgagggggc tcgcccgatc agcggtgata ccggactgcg cgacgtccag
    25681 cgtctggctg aagccaacga ctttcctccc gtcgatgaaa ccaaacgcgg tcgctatcag
    25741 caaatcaacc tgcgtgacgc ttacgttgat cacctgttcg gttatatcaa tgtcaaaaat
    25801 ctcacgccgc tcaagctggt gatcaactcc gggaatggcg cagcgggtcc ggtggtggac
    25861 gccattgaag cccgctttaa agccctcggc gcgcccgtgg aattaatcaa agtacacaac
    25921 acaccggacg gcaatttccc caacggtatt cctaacccgc tgctgccgga atgtcgcgac
    25981 gacacccgca atgcggtcat caaacacggc gcggatatgg gcattgcctt tgatggcgat
    26041 tttgaccgct gttttctgtt tgacgaaaaa gggcagttta tcgagggcta ctacattgtc
    26101 ggcctgctgg cagaagcgtt cctcgaaaaa aatcccggcg cgaagatcat ccatgatccg
    26161 cgtctctcct ggaacactat tgatgtggtg actgctgcag gcggcacgcc ggtaatgtcg
    26221 aaaaccggac acgcctttat taaagaacgg atgcgcaagg aagacgccat ctacggtggc
    26281 gaaatgagcg cccaccatta cttccgtgat ttcgcttact gcgacagcgg catgatcccg
    26341 tggctgctgg tcgccgaact ggtgtgcctg aaaggaaaaa cgctgggcga actggtgcgc
    26401 gaccggatgg cggcgtttcc ggcaagcggt gagatcaaca gcaaactggc gcaacccgtt
    26461 gaggcgatta accgcgtgga acagcatttt agccgtgagg cgctggcggt ggatcgcagc
    26521 gatggcatca gcatgacctt tgccgactgg cgctttaacc tgcgcacctc caataccgaa
    26581 ccggtggtgc gcctgaatgt ggaatcgcgc ggtgatgtgc cgctgatgga agcgcgaacg
    26641 cgaactctgc tgacgttgct gaacgagtaa tgtcggatct tcccttaccc cactgcgggt
    26701 aaggggctaa taacaggaac aacgatgata aatctaaaaa agcgtgagcg agcgaaaacc
    26761 aatgcatcgt taatctctat ggtgcaacgc ttttcagata tcaccatcat gtttgccggg
    26821 ctatggctgg tttgcgaagt cagcggactg tcattcctct acatgcacct gttggtggcg
    26881 ctgattacgc tggtggtgtt ccagatgctg ggcggcatca ccgattttta tcgctcatgg
    26941 cgcggtgttc gggcagcgac agaatttgcc ctgttgctac aaaactggac cttaagcgtg
    27001 attttcagcg ccggactggt ggcgttcaac aatgatttcg acacgcaact gaaaatctgg
    27061 ctggcgtggt atgggctgac cagcatcgga ctggtggttt gccgttcatg tattcgcatt
    27121 ggggcgggct ggctgcgtaa tcatggctac aacaagcgga tggtcgccgt agcgggggat
    27181 ttagccgccg gacaaatgct gatggagagt ttccgtaatc agccgtggtt agggtttgaa
    27241 gtggtgggcg tttaccacga cccgaaaccg ggcggcgttt ctaacgactg ggcgggtaac
    27301 ctgcaacagc tggtcgagga tgcaaaagcg ggcaagattc ataacgtcta tatcgcgatg
    27361 caaatgtgcg acggcgcacg agtgaaaaaa ctggtccatc aactggcgga taccacctgt
    27421 tcggtgctgc tgatccccga cgtctttacc ttcaacattc tccattcacg tctcgaagag
    27481 atgaacggcg ttccggtggt gccgctgtat gacacgccgc tttcgggggt taaccgcctg
    27541 ctaaaacgtg cggaagacat tgtgctggcg acgcttattc tgctgctgat ctccccggtg
    27601 ctgtgctgta ttgcgctggc ggtgaaactc agttcaccag ggccggttat tttccgccag
    27661 actcgctatg gtatggatgg caagccgatc aaagtgtgga agttccgttc catgaaagtg
    27721 atggagaacg acaaagtggt gacccaggcg acgcagaacg atccgcgcgt cactaaagtg
    27781 gggaactttc tgcgccgcac ctcgctggac gaattgccgc agtttatcaa tgtgctgacc
    27841 ggggggatgt cgattgtcgg tccacgtccg cacgcagtag cgcataacga acagtatcgc
    27901 cagctcattg aaggctacat gctgcgccat aaagtgaaac cgggcattac cggctgggcg
    27961 cagattaatg gctggcgcgg cgaaaccgac acgctggaga aaatggaaaa acgcgtcgag
    28021 ttcgaccttg agtacatccg cgaatggagc gtctggttcg atatcaaaat cgttttcctg
    28081 acggtattca agggcttcgt taacaaagcg gcatattgat atgagcttac gtgaaaaaac
    28141 catcagcggc gcgaagtggt cggctattgc cacggtgatc atcatcggcc tcgggctggt
    28201 gcagatgacc gtgctggcgc ggattatcga caaccaccag ttcggcctgc tgactgtgtc
    28261 gctggtgatt atcgcgctgg cagatacgct ttctgacttc ggtatcgcta actcgattat
    28321 tcagcgaaaa gaaatcagtc accttgaact caccactctg tactggctga acgtcgggct
    28381 ggggcttgtg gtatgtgtgg cggtgttttt gttgagtgat gtcatcggcg acgtgctgaa
    28441 taacccggac ctggcaccgt tgattaaaac attgtcgctg gcgtttgtgg tgatccccca
    28501 cgggcaacag ttccgcgcgt tgatgcagaa ggagctggag ttcaacaaaa tcggcatgat
    28561 cgaaaccagc gcggtgctgg cgggcttcac ttttacggta gttagcgccc atttctggcc
    28621 gctggcgatg accgcgatcc tcggttatct ggtcaatagt gcggtgagaa cactgctgtt
    28681 tggctacttt ggacgcaaaa tttattgccc cggtctgcac ttctcgctgg cgtctgtggc
    28741 accgaactta cgttttggtg cctggctgac ggcggacagc atcatccact atctcaacac
    28801 caacctttca acgctggtgc tggcgcgtat tcttggcgcg ggcgtggcag ggggatacaa
    28861 cctggcgtac aacgtggccg tcgtaccgcc gatgaagctg aacccaatca tcacccgcgt
    28921 gttgtttccg gcgtttgcca aaattcagga cgacaccgaa aagctgcgtg ttaacttcta
    28981 caagctgctg tcgatagtgg ggattatcaa ctttccggcg ctgctggggc tgatggtggt
    29041 gtcgaataac ttcgtaccgc tggtattcgg cgaaaagtgg aacagcatta ttccggtgct
    29101 gcaattgctg tgtattgtgg gtctgctgcg ctccgtgggt aacccgattg gttcgctgct
    29161 gatggcgaaa gcgcgggtcg atatcagctt taaattcaac gtattcaaaa catttctgtt
    29221 tattccggcg attgttattg gtgggcagat ggcgggcgca atcggcgtca cgctcggttt
    29281 cctgctggtg cagattatca acaccattct gagttacttc gtgatgatta aaccggttct
    29341 tggttccagt tatcgccagt acatcctgag tttgtggctg ccgttttatc tctcgttgcc
    29401 gacgctggtg gtcagttatg cgctgggcat tgtgctgaaa gggcaactgg cgctggggat
    29461 gctgctggcg gtgcaaatag ccgcgggcgt gctggcgttt gtggtgatga ttgtgctgtc
    29521 acgccatccg ctggtggtgg aagtgaagcg tcagttttgt cgcagcgaaa aaatgaaaat
    29581 gcttttacgg gcggggtgaa ttgctgtttt cggttccgac aacggttgcc ggatgcggcg
    29641 taaaacgcct tatccgtcct acggttctgt gctcgggacc tgtaggcctg ataagacgcg
    29701 ccagcgtcgc atcaggcaaa aaacagcaac gaacacaaca aacacagcct cacctggcat
    29761 caaatttatg aggaaaaaat gaaattactt attctgggca accacacttg cggcaatcgt
    29821 ggcgacagcg ccatcttgcg cggcttactt gatgccatca acatcctcaa tccacatacc
    29881 gaagtggacg tgatgagccg ctatccggtc agttcttcct ggctgctcaa ccgcccggta
    29941 atgggcgatc cgctgttcct gcagatgaca caacacaata gcgcggcggg cgttgtcggg
    30001 cgcgttaaaa aagtcctccg tcgccgctac cagcatcagg tattgctctc acgcgtcacc
    30061 gacactggca agctgcgcaa tatcgccatc gcccagggat tcaccgactt cgtgcgcctg
    30121 ctgtcaggtt acgacgccat tattcaggtc ggcggatcgt tttttgtcga tctctacggc
    30181 gtgccgcagt ttgaacatgc gctttgcaca tttatggcga aaaagccgct gtttatgatt
    30241 ggtcacagcg tcggcccctt ccaggatgag caatttaacc aactggcgaa ctatgtcttt
    30301 ggtcactgcg acgcgctgat cctgcgcgaa tcggtcagcc ttgatttgat gaaacgcagc
    30361 aatatcacca ccgcaaaagt ggaacatggc gtcgataccg cgtggctggt cgatcaccac
    30421 accgaagact tcaccgccag ctatgccgtt caacactggc tggacgttgc cgcacaacag
    30481 aaaacagtgg caattaccct gcgcgaactg gcaccgttcg acaaacgtct cggcaccact
    30541 caacaagcgt atgaaaaagc ctttgccggg gtggtcaatc gcattctcga tgaaggctat
    30601 caggtgattg cgctctccac ctgtacgggt atcgacagct ataacaaaga cgaccgcatg
    30661 gtggcgctca atctgcgcca gcacatcagc gatcctgccc gttaccacgt agtgatggat
    30721 gaactcaacg atctggaaat gggcaaaatt ctcggagcct gtgaactcac cgtcggtacg
    30781 cgcctgcact ccgccattat ctcaatgaac tttgctactc cggcgattgc catcaactat
    30841 gaacataaat ccgccgggat tatgcagcag ctgggactgc cagagatggc gattgatatc
    30901 cgtcatttac tggacggcag cctgcaagcg atggttgcgg ataccttagg ccagcttccg
    30961 gtgctgaacg ctcgacttaa cgaagccgtc agtcgcgaac gtcagacggg aatgcagatg
    31021 gtgcagtctg tgcttgagcg catcggggag gtgaaatgaa ggtcggcttc ttcttattga
    31081 aattcccgct gtcgtcggaa accttcgtcc tcaatcaaat taccgcgttt attgatatgg
    31141 gctttgaagt ggagattgtc gctttgcaaa aaggcgatac agagaacacc cacgcggcat
    31201 ggacgaaata caaccttgtc gccagaaccc gctggttaca ggacgaacct gcgggcaaag
    31261 tagcgaaact gcgccaccgc gccagccaga ccttacgtgg cattcatcgt aaaaatacct
    31321 ggcatgcgct caacctcaaa cgctatggtg ccgagtcgcg gaacctgatt ttgtctgcca
    31381 tttgtggtca ggtcgcaaca ccgtttcgcg ccgatgtgtt catcgctcat tttggcccgg
    31441 cgggggtaac cgcagcaaaa ctacgcgaac tgggtatcat tcgcggcaaa attgccacca
    31501 tcttccacgg tattgatatc tccagtcggg aagtgctcaa ccactacact cccgaatatc
    31561 aacaactgtt tcgccgtggc gacctgatgt taccgataag cgatctgtgg gccggaaggc
    31621 tgcaaaaaat gggctgcccc agagaaaaaa tcgccgtatc gcgtatgggc gtggacatga
    31681 cgcgctttag cccgcgtccg gtgaaagcgc ccgcaacgcc gctggaaatc atatctgtcg
    31741 cacgcttaac cgagaaaaaa ggcctgcatg tggcgattga agcctgccgt cagttgaaag
    31801 agcagggcgt ggcatttcgc tatcgcatcc tcggcattgg cctgtgggaa cgacgcctgc
    31861 gcaccctcat cgaacaatat caactggaag atgtggtaga gatgccgggc tttaaaccga
    31921 gccatgaagt gaaggagatg ctcgacgacg cggatgtctt cctgttgcca tcggtaacgg
    31981 gcgcggatgg cgatatggaa ggcattccgg tggcgctaat ggaagcgatg gcggtcggta
    32041 ttccggtggt ttctactctg catagcggaa ttcctgaact ggtggaggcc gataaatccg
    32101 gctggctggt gcctgagaac gatgctcgcg cactggcaca acgtctggcg gcgtttagcc
    32161 aactggatac cgacgaactg gctccggtcg tcaaacgtgc gcgcgaaaaa gtcgaacacg
    32221 attttaacca gcaggtgatt aatcgagaac tcgccagctt gctgcaggct ttatagagga
    32281 gaacgtatgc catttaaaac actctcccga cgcaccttcc tgacggcaag ctcggcgctt
    32341 gccttcctcc ataccccttt cgctcgcgca cttcccgccc gacaaagcgt taacattaac
    32401 gactacaacc cacatgactg gatcgcctca tttaaacaag ccttcagcga agggcaaacg
    32461 gtcgtcgtgc ctgccggatt cgtttgtgac aatatcaaca ccggcatctt catccctccc
    32521 ggcaaaacgt tacacatcct tggaagcctg cgcggcaacg gcagagggcg atttgtctta
    32581 caggacggca gccaggtgac agggggagag ggcggcggta tgcataacat taccctggat
    32641 gtgcgtggtt ctgactgcac catcaaaggg ctggcgatga gcggctttgg cccggtaatg
    32701 cagatttata ttggcggcaa aaacaaacgg gtcatgcgca acctgaccat cgataacctc
    32761 actgtcagcc acgctaatta cgccatctta cgccagggat ttcataacca gattatcggt
    32821 gccaacatta ccaactgtaa gttcagcgac ttacagggcg acgccattga atggaacgtg
    32881 gcaattaacg acagtgatat tttgatatct gaccatgtca tcgagcgtat caactgtacc
    32941 aacggcaaaa tcaactgggg catcggcata ggccttgcgg gaagcactta tgataacaac
    33001 tacccggaag accaggcagt gaaaaacttt gtcgtggcga atatcacggg atcggattgt
    33061 cggcagttga tccatgttga aaatggtaaa cattttgtta ttcgtaatat caatgcccgc
    33121 aatatcacgc cggatttcag taagaaagcg ggcattgata acgcgacagt cgctatttac
    33181 ggttgtgaca atttcgtgat tgataatatt gaaatgatta atagcgccgg gatgttaatc
    33241 ggctatgggg taattaaagg caaatatctc tcgataccgc aaaatttccg agtgaataat
    33301 attcaactgg ataataccca tcttgcttat aaattgcgcg gcatccaaat ctccgccggg
    33361 aatgccgtct cctttgtgtc gctaactaac attgagatga agcgtgcctc gctggagtta
    33421 cacaacaagc cgcaacatct ttttatgcga aatatcaatg tgatgcagga atcctcagtt
    33481 ggaccagcat tgagcatgaa cttcgacatg cgcaaagacg ttcgcggcgt ctttatggcg
    33541 aaaaaagaaa cactgctgtc tcttgcaaat gttcatgcgg tgaatgaaag agggcaaagc
    33601 tccgtcgata tcgacaggat taatcaccat attgttaatg tggaaaagat taactttaga
    33661 ttgccggaac ggagggagta gatttgcgac cattcctgga aaaatggagc catacttagg
    33721 aacaatgcta ctgcaatcca caacgaagcg gcgtaacatc acaagtaatt cagtaatcaa
    33781 ttcagggtaa ttgatgctgg cgaaaaaaat cgaacaagct ataattcagc aaccatttta
    33841 caggtggatg aaataatgac gaatttaaaa gcagttattc ctgtagcggg tctcgggatg
    33901 catatgttgc ctgccactaa ggcgataccc aaagagatgc taccaatcgt cgacaagcca
    33961 atgattcagt acattgttga cgagattgtg gctgcaggga tcaaagaaat cctcctggta
    34021 actcacgcgt ccaagaacgc ggtcgaaaac cacttcgaca cctcttatga gttagaatca
    34081 ctccttgagc agcgcgtgaa gcgtcaactg ctggcggaag tacagtccat ctgtccgccg
    34141 ggcgtgacca ttatgaacgt gcgtcagggc gaacctttag gtttaggcca ctccattttg
    34201 tgtgcgcgac ctgccattgg tgacaaccca tttgtcgtgg tactgccaga cgttgtgatc
    34261 gacgatgcca gcgccgaccc gctacgttac aaccttgctg ccatgattgc acgtttcaac
    34321 gaaacgggcc gcagccaggt gctggcaaaa cgtatgccgg gtgacctctc tgaatactcc
    34381 gtcatccaga ctaaagagcc gctggaccgt gagggtaaag tcagccgcat tgttgaattt
    34441 atcgaaaaac cggatcagcc gcagacgctg gactcagaca tcatggccgt aggtcgctat
    34501 gtgctttctg ccgatatttg gccggaactg gaacgtactc agcctggtgc atggggacgt
    34561 attcagctga ctgatgctat tgccgagctg gcgaaaaaac aatccgttga tgcaatgctg
    34621 atgaccggcg acagttacga ctgcggcaaa aaaatgggct atatgcaggc gtttgtgaag
    34681 tatggcctac gcaacctgaa agaaggggcg aagttccgta aaggtattga gaagctgtta
    34741 agcgaataat gaaaatctga ccggatgtaa cggttgataa gaaaattata acggcagtga
    34801 agattcgagg cataagtttt gctcattgaa ttttcctgcc gttgccgttt ttataaagcc
    34861 tataaaaaca agaagttaca tagctcatat tattcaaatt tttccaggat tttccttgtt
    34921 tctacggctg tttggtaaga caattaacgt ttgaattttt cggatttggc gcgagtggct
    34981 aacgctcgcc acatcgttga gatgcatgca gtgcactggt agctgttaag ccaggggcgg
    35041 tagcgtgtat taatacctct attaatcaaa tctggagcag tctatttcac agcatgctct
    35101 atagctatat ggaataaaaa agtgaagata cttgttactg gtggcgcagg gtttattggt
    35161 tctgctgttg ttcgtcacat aataaataat acgcaagata gtgttgttaa tgtcgataaa
    35221 ttaacatacg ccgggaacct ggaatcactc gctgatgttt ctgattctga acgttatgct
    35281 tttgagcatg caggtatctg cgatgctgta gccatgtcgc gtattttcgc acagcaccag
    35341 ccagacgcgg tgatgcacct ggcggcagag agccacgttg accgttcaat aacgggccct
    35401 gcggcattta ttgaaaccaa tattgtgggg acttatgttc ttttagaagc ggcgcgcaat
    35461 tactggtctg ctctgaatga tgaaaagaaa aaaagtttcc gctttcatca tatttctact
    35521 gatgaagttt atggtgactt accccatccg gatgaagcaa ataataacga agcgttaccg
    35581 ctatttacgg aaacgacagc ttacgcgcca agtagcccgt attctgcgtc taaagcttcc
    35641 agcgatcatt tggtacgtgc ctggaagcgg acttatggtt taccaaccat tgtgactaat
    35701 tgctcgaaca attatggtcc ttatcatttc ccggaaaaac ttattccatt ggttattctg
    35761 aatgctctgg aaggtaaggc attacctatt tatggtaaag gggatcaaat tcgcgactgg
    35821 ttgtatgtag aggatcatgc tcgtgcgtta tataccgtcg taaccgaagg taaagcgggt
    35881 gaaacttata acattggtgg acataacgaa aagaaaaaca tcgacgtagt gctcactatt
    35941 tgtgatttgt tggatgagat tgtaccgaaa gagaaatctt atcgtgagca aattacttat
    36001 gttgccgatc gcccggggca tgaccgccgt tacgccattg atgccgataa aattagccgc
    36061 gaattgggct ggaaaccgca agaaacgttt gagagcggga ttcgtaaaac ggtggaatgg
    36121 tatctggcta atacaaattg ggttgagaat gtgaaaagcg gtgcttatca gtcgtggatt
    36181 gaacaaaact atgagggccg tcagtaatga atatcctgct tttcggcaaa acagggcagg
    36241 tgggttggga actgcagcgt gctctggctc ctctgggtaa cttgattgcg ctcgatgttc
    36301 actccactga ttattgtgga gatttcagca accccgaagg tgtggctgaa accgtcaaaa
    36361 aaattcgccc cgatgttatt gttaatgctg ctgctcacac tgcagtagac aaagcggaat
    36421 cagaaccgaa tttcgcgcaa ttacttaacg cgacatgcgt cgaagcgatt gcaaaagcag
    36481 ctaatgaagt cggagcctgg gttatacact actccactga ttatgttttc ccaggcaacg
    36541 gtgacacgcc atggctggaa acggatgcaa cagcaccgct aaatgtctac ggtgaaacca
    36601 agctagctgg ggaaaaagcg ttacaagaac attgcgcaaa gcatcttatt ttccgtacca
    36661 gctgggtata cgctggtaaa ggaaataact ttgccaaaac gatgttgcgc ctggcaaaag
    36721 aacgcgaaga gttggctgta ataaacgatc aattcggcgc accaacgggg gctgaattac
    36781 tggctgattg caccgctcat gccattcgcg tggccgcaaa taaaccagaa gttgctggtt
    36841 tgtaccatct ggttgctggt ggtacaacaa cctggcacga ttatgccgct ctggtatttg
    36901 aagaagcgcg cagagcgggg atcaaccttg ctcttaacaa acttaatgcc gtgccgacaa
    36961 cggcctatcc cacaccagca cgtcgtcccc ataattctcg cctcaatacc gaaaaatttc
    37021 agcagaactt tgcgcttgtc ttgcctgact ggcaggtggg cgtgaaacgt atgctcaacg
    37081 aattatttac gaccacggca atttaacaaa ttattgcatc tcgctcatga tgccagagcg
    37141 agatgaatta aaaggaatgg tgaaatgaaa acgcgtaaag gtattattct ggctggtggt
    37201 tccggcactc gtctttatcc tgtgactatg gctgtgagca aacaactgct accgatttat
    37261 gataaaccga tgatttatta tccgctttca acgcttatgt tggcgggtat tcgagatatt
    37321 ctgattatca gtacgccgca ggatacacca cgttttcaac aattgcttgg tgatggaagc
    37381 cagtgggggc ttaatctgca atataaagta caaccgagtc cggatggtct ggcgcaagca
    37441 tttatcattg gtgaagaatt tataggtggt gatgattgtg cgctggtact tggtgataat
    37501 atcttctatg gtcacgacct gccgaaatta atggataccg ctgttaacag agagagtggc
    37561 gcaacggttt ttgcctatca cgtaaatgat ccagagcgct acggtgtggt ggaatttgat
    37621 gataacggta cggcaattag tctggaagaa aaaccgctgg aaccaaaaag taactatgcg
    37681 gttactgggc tttatttcta tgacaatgac gttgtagaaa tggcgaaaaa ccttaaacca
    37741 tctgcacgtg gcgaactgga aattaccgat attaaccgta tttatatgga ccaaggacgt
    37801 ttgtctgtcg ctatgatggg gcgtggttat gcatggctgg acacggggac acatcaaagt
    37861 cttattgaag caagcaactt cattgcaaca attgaagagc gtcaggggct gaaagtttcc
    37921 tgcccggaag aaattgctta ccgtaaaggc tttattgatg ctgagcaagt gaaggtatta
    37981 gccgagccgc tgaagaaaaa tgcttatggt cagtatctgc tgaaaatgat taaaggttat
    38041 taataaaatg aacgtaatta aaactgaaat tcctgatgtg ctaatttttg aaccaaaagt
    38101 atttggtgat gagcggggct tttttatgga aagcttcaat cagaaagttt tcgaagaggc
    38161 tgtaggacga aaggttgaat ttgttcagga taaccattct aaatcaacta agggtgtgtt
    38221 acgcggactg cactatcagc tggaacctta tgctcaagga aaactggtac gctgtgttgt
    38281 cggtgaagtt tttgacgtag ctgttgatat tcggaaatca tcaccaacat ttggcaagtg
    38341 ggttggtgta aatttgtcgg cggagaataa gcgtcaatta tggataccgg aaggattcgc
    38401 tcatgggttt tgcgttctta gtgatgaagc agaatttgtc tacaaaacaa ataatttcta
    38461 ttcaaaaatg caagagcgtg ggattttatg gagcgacaaa agtataaata tagaatggca
    38521 ggttcaaaat ccattgcttt ctgataaaga tattaatggt caaaaatttg tagatgctga
    38581 ttattttata tgataaagaa atgtaataat gagcataata aaaaatagtg tctggaacct
    38641 ttttggctat gcaataccaa ctttaattgc tatcccctcg ctaggatttc tcgctcgagg
    38701 tttggggcct gaaggtttcg gtgtttatac aattgcaatt gcacttgttg ggtatgctgg
    38761 aatttttgat gtaggcctga ctcgctctgt tattagagaa attgcaattc atcgtgataa
    38821 tcatcatgaa agaaccaagg taatttcaac aagtacatct tttttggtgc tattttcatg
    38881 ctttggtgct tttttattat tgattttctc tgatggaatt gttaattatc taaaaatttc
    38941 tggtgttgaa catagtgata tacaactagc atttaaactg ttggctattt gcattccatt
    39001 atttattcta aatcaattat ggtcagccat tcttgagggg gatgaaaaat ttggcattgt
    39061 aaatattcaa aaatctatat caagctcttg catcgcggga attccggcca tatttgtttt
    39121 ttatagtgct acattgtcgg cagcggttgc tggtttaata tttgcaagag ttatttcgat
    39181 tttagtctct gcctattatg tcaggaatga tattaaaatt tcgggggttc atttttgtta
    39241 taaaaccttt aaacgactct ttttctttgg cggttggatg acagtaagta atatcataag
    39301 tccggtcatg gtgtattttg atcgatttat agtgtcaaat attatggggg cagataaagt
    39361 tgcattttat tctgcaccag cggaggttat tctaaaatta ggaataatac ctgcagcaat
    39421 cgggagggca gtgtttccaa ggttaagtaa catcaaagac tttaaagaat ttaaacgtaa
    39481 tgtaaataaa tcattgcttt taatgtttct aatctgtttg ccggtgataa tcataggctt
    39541 gttatattca gggcttgtat tgaaaatatg gtttggtgag aattaccaaa ttaattcctt
    39601 taatatatta aatgtgttat tgatcggttt tttcttcaat gcgctggcaa tgataccatt
    39661 ctctgcaatc caggcattag gaaaatctaa aattactgct ttgattcatt gtgctgaatt
    39721 ggttccttat ttagcccttt tgtattttat ggtcgaaaaa tatgggttac tgggtgccgc
    39781 aatatcctgg agcatacgtg taattttaga tgctctgtta ttacaatggc tttatactag
    39841 aatgtgttct gtatatgaaa actaaaatta accaattact gagtcacttg gataatgaat
    39901 agtaatattt acgctgtcat tgtgacatat aatcccgaac ttaaaaatct gaatgcactg
    39961 atcactgaac taaaggagca aaattgttac gttgtagtag tcgataatag tactaatttc
    40021 accttgaaag ataaattagc agacattgaa aaagttcatt taatttgtct tggtaggaat
    40081 gaaggtatag caaaagcaca aaatattggg attcgatatt ctcttgaaaa aggggcagag
    40141 aagataattt tttttgatca agatagccgt attcgcaatg agtttatcaa aaaactttcc
    40201 tgctatatgg ataatgaaaa tgcaaaaata gctggcccag tttttattga tagagataag
    40261 tcacattatt atcctatttg taatatcaaa aaaaatggtc ttcgagagaa aattcatgtc
    40321 actgaaggac agacaccgtt taaaagttca gtaacaatct catccggaac catggtttca
    40381 aaagaagttt ttgagattgt tggaatgatg gatgaggaac tttttattga ttatgtcgat
    40441 acagaatggt gtcttagatg cttaaactat ggcatattag ttcatatcat tcctgatata
    40501 gaaatggttc atgctattgg ggataagtca gtaaaaatct gtgggattaa cataccaatt
    40561 cactcgccag tacgtcgtta ttatcgagta agaaatgcat ttcttttgct tagaaaaaat
    40621 catgtgcctc ttttactctc tattagggaa gttgtttttt ctttaattca tacgacttta
    40681 attatcgcaa ctcaaaaaaa taaaattgaa tatatgaaaa aacatatttt ggctacgctg
    40741 gatggaataa gaggaattac tggggacggt cgttataatg catagttctg atcaaaaaag
    40801 agtagctgta cttatggcta cgtataatgg cgaatgctgg attgaggaac aattaaaaag
    40861 tattattgaa caaaaagatg tagacatctc aatttttata tcagatgatt tatcaactga
    40921 taatacatta aatatttgtg aagaatttca attatcatat ccctcaatta taaatatact
    40981 tccaagcgtc aataagtttg ggggggcagg taaaaatttt tatagattaa ttaaagatgt
    41041 cgatcttgaa aactatgatt atatctgttt tagtgatcag gacgatattt ggtataaaga
    41101 taaaataaaa aatgcaattg actgcttggt atttaataat gcgaattgtt attccagcaa
    41161 tgtaattgca tattatccat cagggcaaaa aaatttagtt gataaggctc agtcgcaaac
    41221 tcaattcgat tatttttttg aagcagcagg tccggggtgt acatatgtta taaaaaaaga
    41281 aacgcttatt gaatttaaga agttcatcat aaataataaa aatgctgcac aagatatctg
    41341 tctgcatgat tggtttttat attcatttgc gcggacgaga aattattcgt ggtatattga
    41401 tagaaaacca acaatgctgt acagacagca tgaaaacaac caagtgggag caaacattag
    41461 ttttaaagca aaatacaaac ggcttggttt ggtgagaaac aagtggtatc gaaaagaagt
    41521 aactaaaata gctaatgcct tagctgatga tagctttgtg aataatcaat tgggtaaagg
    41581 ttatataggc aatttgatct tggctttaag tttttggaag ttaaggcgga aaaaggctga
    41641 taaaatttat attttattaa tgttgatttt aaacatattt taaaaaacat caccttacga
    41701 atatgaataa tataaataaa atttttataa catttttatg tattgaactg attattggtg
    41761 gtggtggaag attactggag ccattgggaa tattcccttt gcgatattta ttatttgtat
    41821 ttagttttat acttttaatt tttaatttag ttacattcaa tttttcaatc acccaaaaat
    41881 gtgtcagtct ttttatatgg ttgcttttat ttccttttta tggcttcttt gtcggcttat
    41941 tagctggtaa taaaataaat gatatactgt ttgatgtgca accatacctc tttatgctgt
    42001 cacttatata tctatttaca ctaagatata ctttaaaagt atttttatgt gagattttta
    42061 ttaaaatagt taatgcattt gcattatatg gatcactgtt atatatttca tacataattt
    42121 tgttgaattt cggtttgtta aattttaatt taatttatga acacttatca ttgactagcg
    42181 agttcttttt tcgtcccgat ggggcttttt tttccaaatc cttctacttt tttggtgtcg
    42241 gtgcgattat cagttttgtc gacaaaaaat atttaaaatg tctcataata gtgcttgcga
    42301 tattattgac agaatcaaga ggtgtattac tttttacaac attatcactg ttattagcca
    42361 gttttaaatt acataagcta tatttaaata ctattataat aatattgggc agcgttctat
    42421 ttataattat gctttacatg gtcggatcac gcagtgaaga ttctgactct gttagattta
    42481 atgatttata tttttattat aaaaatgttg atttagcgac gttcttgttt ggaagaggat
    42541 ttggttcatt tatattagat cgattaagga ttgaaatagt acctcttgag atacttcaga
    42601 aaacaggcgt tattggtgta tttatatcat tagttcctat gttgcttatc tttttgaaag
    42661 gctatttttt aaacagtgca aaaacatcat taatgatgtc gttaatactt tttttcagta
    42721 ttaccgtttc tataactaat ccatttcttt ttacacccat gggaattttt attataggcg
    42781 ttgtagtttt atgggtattt tctatagaaa atatccaaat tagtaataac ctcacttctg
    42841 gagcaaaata aaggatgtta aaaataggga agttattgac cagttctttt tttagttatt
    42901 ttttaattgg aattgtcaat actgctttac attggggggt attttatgcg tgttataata
    42961 atttagcttt tggtcaaggc cgaagtaaca ttgttggttt tatatgtgca gcaacctttt
    43021 ctttttttgc aaatgcaaga tgctcattta aagtatctgc aaccaaggct agatatttta
    43081 tatttatatt ttttatgggg gcgatgagtt atttattcgg cgttttattt gatctcttag
    43141 ctttatctcc aatatttaca ttgttcacct tttctctttt tagtttggtt ttaggatatt
    43201 gcgcatcaaa atatttcatt tttcgttagt aaacgatttg ttaaaaacac aattacttgc
    43261 acattagcta aatagtttat atgaggggct tatgtatcac tcttttaata tcgctgtaat
    43321 tattccttgt tataatgagc agaaggcaat agcaaaagtc ataaatgatt tcaaaacgaa
    43381 tattccaact gcttctatat atgtatttga taataattcg actgacagta cagcacaagt
    43441 ggcagaggat gctggagcaa cagttcactc tgttcctctg aaaggcaagg ggaatgtcgt
    43501 aagacgtatg ttctcagatg tagatgcgga tatttatcta atggttgatg gagatgacac
    43561 ttatgatgca agttctgctc cagaaatgat taaccatctc ataaaaaatc aattggatat
    43621 ggttgtagga tgtcgtcaag agaatggaga tcaaaataca tacagaaaag ggcatcgctg
    43681 aggaaataaa ctgttgactg gtactgttca gactatcttt aaagggaatt ttactgatat
    43741 gttgtcgggg tatcgtgtat tttccagacg ctatgttaaa tcttttccat gtctatcaag
    43801 gggatttgaa atcgaaactg agcttacaat tcatgcttta gaattacgta tgaagtatgg
    43861 tgaggttaat acaaaatatg gcgagcgatc tgaagggagt gtgagcaaac tatcaacttg
    43921 gagtgatggg ttcaagattc tgaaaacaat aattaaactt tactcgcttg aacggccact
    43981 atactttttt agtattatcg gcgttttact tgctgctttg tctataattt taggattgcc
    44041 catcattgtt gattatattg atacaggttt ggtgagaaga tttccgactg cctttttaac
    44101 tgcctccatc atgttatcgt caataatggc atttgtatgt ggcattattt tgcattcaaa
    44161 tacaacaact agacgtgaaa tgaaggcatt attttatctt tctgaaaaaa aactataaac
    44221 tgataatgta aatagagtat tttactatga ttgaattgcc gttgtatttg tatgttgtgt
    44281 attgttttgc ttttggaatg acattttttg tcattggtaa tattgtattg tcatcttttt
    44341 ttaaggaggg ggtggcagga cttaacaaat tttctttatt tttttattgc ctatgcatag
    44401 ggatgtcttt aagtattttg ataatttact ttctgggcat ggtcgggcaa tttaataaga
    44461 ttgctatctt tttaattttt acggtgtgtt gggttttaag cattattaaa cgtcagcaat
    44521 ttagatggct ggcaataaat aatattgagt tcagtacgct gtttgttatt ttatttcttg
    44581 ttttaatttt tgcggttaca ttgttatcgt ccctgcgagc tcccggtgat tgggatgata
    44641 ctatgtatca cttacctttg gctcgttcat tagttgagca ccatgcaatt gttgttgaac
    44701 aatatttgcg ttttcctctg ttcccgcaaa atgcagatct tttaatggct ttaggtttac
    44761 agttaggaga tgtaaggtta gctcagtttt tagcaaatat ctgttttttt gtaatagcct
    44821 gtggacttgt tggctgttca tgggaaatta cgaaaactta ttatcttggc attattgcta
    44881 caatcctact atttactatc aatccactta aagatcatct tggttatgca tatattgatt
    44941 taacgctatc tttgttttgc tgcagccagt attcttatat atattcgctc agaaaacaat
    45001 aaaacacaaa atgaattaat ttttttatgt ggttttttta ttggaattgc ttgtggcgta
    45061 aaactatttg gtgccatata tggagccatc atatttgtta tattgatagc gaaaaagaga
    45121 aacatacaga catttcttat ttttagcttg gcaacaattc ttggtggcat ttattggtat
    45181 gtccgtagtt tttggatctc tggtgatcct tttagtcctg ctggaggaaa tatatttggt
    45241 cactacctat ggaatgaaat tgatctacaa gttcaaacag cagaacaagc tcgacatggt
    45301 ataagtcctg ccacattaga tttgcaacaa gcaattaata aagttggcag tgatgttgtt
    45361 ttttatgcta tactgtctgt ctttacactt aggaataata aaatactttg ggtatttttc
    45421 tcgattgttt taacatatgt cttattttgg ttttctgtta cgcaaggtga caggtattta
    45481 tcacccattt acccattgtc agtattgctg gttgcgatct caattgcaag tttaataaaa
    45541 gatatcaaca ttacaaatta caagttggga cgatatttta tccttgtgtg tattattttt
    45601 tttcggggcg atcgttgtat tttgaattca aatattttta ttctgaatac ttgaatttta
    45661 attcacgctt agaaagtcgc tatagctatg aactgatgaa gaaagcaagt gaatatagtg
    45721 agttatatgg agataatctt atccaattag gcttggagga tggtatttac ttctataaag
    45781 ggatggcaat tggtgacgtc tttggattag caagatatag tgattggact attagtaatc
    45841 ccgagtgtga ggtaatccca caggatgatt taatagagaa gatgaaatct tttaatagtt
    45901 cgtttatttt tatatctaaa cgaagctatg ctaattttaa tccagaaaag tatccaaaat
    45961 tcaaagttct tatggataca cccaatggca tacttatcgc tataaagtaa gaagcatgct
    46021 gtaaccatat gtattccttg attagaagat gaaaatcacg acggttaata atataacggt
    46081 taatagacgg ttaataatat agaagacagg agtaaacaat gtcaaagcaa cagatcggcg
    46141 tagtcggtat ggcagtgatg gggcgcaacc ttgcgctcaa catcgaaagt cgtggttata
    46201 ccgtctctat tttcaaccgc tcccgcgaaa agacggaaga agtgattgct gaaaatccag
    46261 gcaagaaact ggctccttac tatacggtga aagagtttgt tgaatctctg gaaacgcctc
    46321 gtcgcatcct gttaatggtg aaagcaggtg caggcacgga tgctgcgatt gattccctga
    46381 aaccatacct cgataaaggt gacatcatca ttgatggtgg taataccttc ttccaggaca
    46441 ccattcgtcg taatcgtgag ctttcagcag agggctttaa cttcatcggt accggtgttt
    46501 ccggcggtga agagggcgcg ctgaaaggtc cttctattat gcctggcggg cagaaagagg
    46561 cttatgagct ggttgcgcct atcctgacta aaatcgctgc cgtggcagaa gacggcgagc
    46621 catgcgttac ttatattggt gccgacggcg caggccatta tgtgaagatg gttcacaatg
    46681 gtattgaata cggagatatg cagctcattg ctgaagcgta ttctctgctc aaaggtggcc
    46741 tgaatctatc taatgaagaa ctggcccaga ctttcaccga gtggaataac ggtgaactta
    46801 gcagctacct gatcgacatc actaaagata tcttcaccaa aaaagatgaa gacggtaact
    46861 acctggttga tgtgattctg gatgaagcag caaacaaagg caccggtaaa tggaccagcc
    46921 agagcgcgct ggatctcggc gaaccgctgt cgctgattac cgagtctgtg tttgcacgtt
    46981 atatctcttc tctgaaagat cagcgcgttg ccgcatctaa agttctctct ggtccgcaag
    47041 ctcagtcagc aggcgacaag gctgagttca tcgaaaaagt tcgcagtgcg ctgtatctgg
    47101 gcaaaatcgt ttcttacgct cagggcttct ctcagctgcg tgctgcgtct gaagaataca
    47161 actgggatct gaactacggc gaaatcgcga agattttccg tgctggctgc atcatccgtg
    47221 cgcagttcct gcagaaaatc accgatgctt atgccgaaaa tccgcagatc gctaacctgc
    47281 tgctggcccc gtacttcaag caaattgccg atgactacca gcaggcgctg cgcgatgtcg
    47341 tcgcttatgc agtacagaac ggtatcccgg ttccgacctt cgccgctgcg gttgcctatt
    47401 acgatagcta ccgtgccgcg gttctgcctg cgaacctgat ccaggcacag cgtgactatt
    47461 tcggggcgca tacttataag cgcattgata aagaaggtgt gttccatact gaatggctgg
    47521 attaatctga tttaaccaac aataaaattg aggcccggcg tatattgcac cgggcttttt
    47581 tttgccaaat atcttataga ctaaatttca ctgcttaata ttaacttaat aaacatcagc
    47641 tattcttata aagaaaatct gaattgtttt tcgttgcgtt gaccatctga caacgtagcg
    47701 ttaaaacttt tagcatctta tcacgatgct aaaaacatca tgattcacag ttaagttaat
    47761 tctgagagca tgaaatgaaa atcaccattt ccggtactgg ctatgtaggc ttgtcaaacg
    47821 ggcttctaat cgcacaaaat catgaggttg tggcattaga tattttaccg tcacgcgttg
    47881 ctatgctgaa tgatcggata tctcctattg ttgataagga aattcagcag tttttgcaat
    47941 cagataaaat acactttaat gccacattag ataaaaatga agcctaccgg gatgctgatt
    48001 atgtcatcat cgccactcca accgactatg atcctaaaac taattatttc aatacatcca
    48061 gtgtagaatc agtaattaaa gacgtagttg agataaatcc ttatgcggtt atggtcatca
    48121 aatcaacggt tcccgttggt tttaccgcag cgatgcataa gaaatatcgt actgaaaata
    48181 ttattttctc tccggaattc ctccgcgaag gtaaagccct ttacgataat ctccatccgt
    48241 cacgtattgt catcggtgag cgttcagaac gcgcagaacg ttttgctgcg ttgttacagg
    48301 aaggcgcgat taagcaaaat atcccgaccc tgtttaccga ctccactgaa gcagaagcga
    48361 ttaaactttt cgctaatacc tatctggcga tgcgcgtagc atactttaat gaactggata
    48421 gttatgcaga aagtttaggt ctgaatactc gtcagattat cgaaggcgtt tgtctcgatc
    48481 cgcgtattgg caaccattac aacaatccgt cgtttggtta tggtggttat tgtctgccga
    48541 aagataccaa gcagttactg gcgaactacc agtctgtgcc gaataacctg atctcggcaa
    48601 ttgtcgatgc aaaccgcacg cgtaaagatt ttattgccga tgccattttg tcacgcaaac
    48661 cgcaagtggt gggtatttat cgtctgatta tgaagagcgg gtcagataac ttccgtgcgt
    48721 cttctattca ggggattatg aagcgtatca aggcgaaagg cgttgaagtg atcatctacg
    48781 agccggtgat gaaagaagac tcattcttca actctcgcct ggaacgtgat ctcgccacct
    48841 tcaaacaaca agccgacgtc attatctcca accgtatggc agaagagctt aaggatgtgg
    48901 cagataaggt ctacacccgc gatctctttg gcagcgacta acatcttgtt atcagggcta
    48961 tttacgccct gattgtcttt tgttacctcc gcaataattc attattttta tcacttatcc
    49021 tatagcattc acggggatta tcgctaaact atgcggactt ggaaatttcc gtcagttagg
    49081 gtaatgatga gagtagaaaa taataatgtt tctgggcaaa accatgaccc ggaacagatt
    49141 gatttgattg atttactagt gcagttgtgg cgtggcaaga tgacaattat catttccgtc
    49201 attgtggcta ttgccctggc tattggttat ttggcagtag cgaaggagaa atggacgtca
    49261 acagcaatta tcactcagcc cgacgtgggg caaattgctg gctataacaa tgccatgaat
    49321 gttatctatg gtcaggctgc accgaaagta tcggatttgc aggagacgtt aattggtcgc
    49381 ttcagttctg ccttctctgc attagcagaa acgctggata atcaggaaga gccagaaaaa
    49441 cttaccatcg aaccttctgt taagaaccag caattaccat tgactgtttc ttatgttggg
    49501 caaactgcag agggcgcaca aatgaagttg gcccaataca ttcagcaagt tgatgataaa
    49561 gtgaatcaag agctagaaaa ggatctcaag gacaacattg ctctgggacg gaaaaacttg
    49621 caggactctt taagaaccca ggaagtggtc gcgcaggagc agaaagatct gcgtatccgt
    49681 cagattcagg aagcgttgca gtatgcgaat caggcgcagg tgacaaagcc acaggttcag
    49741 cagactgaag atgtgacgca agatacgttg ttccttctag ggagcgaagc gctggagtcg
    49801 atgattaagc atgaagcgac tcgtccgttg gtgttctcac caaactacta tcagacacgt
    49861 caaaacctgt tggatattga aaaattaaag tttgatgatc ttgatattca tgcttaccgc
    49921 tatgtgatga aaccgacgtt acctattcgt cgcgatagtc cgaaaaaggc aatcaccttg
    49981 attctggcag tgcttctggg cggcatggtt ggcgcgggga ttgtgttggg gcgtaacgct
    50041 ctgcgtaatt acaacgcgaa gtaatattat tgtgcattta agagaaacgg gcagggtggt
    50101 gacaccatgc ccgttttttt tgccggatgc gatgctggcg catcttatcc ggcctacgtg
    50161 tgttgagata atgtgtaggc acgataagtt tgcgcatcgg gcaatggctc cgggtgtgac
    50221 aacaacatca cacctgctca gcccgtaact cactgatgcc gtttacgcag gttctcaatt
    50281 actgtcgtta aatccagatc ctgatcctgc aacagcacca gcaggtgata catcaagtca
    50341 gatgcctcgt tggtcagctc aaagcgatca tgtaccgttg ctgccagtgc ggtttccacg
    50401 ccttcttcgc ccactttctg cgcaatgcgt ttagtgccgc tggcatacag tttggcggtg
    50461 taggaggttt ccgggtcggc atatttacgt tcggcgagca gttgttccag ttgatacagg
    50521 aacagccact ggtgagcggt gttgccgaag cagctactgg tgcctttgtg gcaggttggc
    50581 ccgatggaat tcgccagcac cagtaatgtg tcgttgtcgc agtccggggc aatacttacc
    50641 acattgagaa aattgcccga ggtttcgcct ttggtccaca gtcgctgttt agtgcgcgag
    50701 aagaaggtga ctttaccgct ttcgatggtt ttgtctaagg cttccgggtt catgtagccc
    50761 agcattaaca cttcgccaga taccgcgtgt tgcacaatca ccggcatcag accgtcggtt
    50821 ttttcccagt ccagttcgcg acgttgttgt tctgttaaca tatcctgatc tccacgccct
    50881 gtgttgccag gtacgctttt aattcgccaa tattgattat ttgtttgtgg aatacggaag
    50941 ctgccagcgc accgtcaacg tcggcatcgc ggaaggcttc caggaagtgt tccatggtgc
    51001 ccgcgccacc ggaggcaatc agcgggacgt ggcaaacttc acgcactttt ttcagctgct
    51061 tgaggtcgta accgttacgc acgccgtcct gattcatcat gttgaggacg atttctccgg
    51121 cgccgcgttt ttgcacctcc tctacccagt cgagcgtttc ccattgagtg acgcgggtgc
    51181 ggctttcatc gccggtatat tgatttacat gatatttacc ggtttcggcg tcgtaccagg
    51241 tatcaatacc aaccacaata cactgcacgc caaagcgatc ggccaggcga gtaattaatg
    51301 ttgggtctgc cagcgcaggg gagttgatgg aaattttatc cgcgccaaag gaaagaattt
    51361 tcgcggcatc ttccagagac ttaatcccac ccgccacaca aaacggaatg tcgatcactt
    51421 ccgccacgcg agatacccag cttttatcta ccacacggcc atcgctggaa gcggtgatat
    51481 cgtagaacac cagttcatct gcaccttctt cggcgtagcg ttttgccagt ggcacgatat
    51541 cgccaatgat ttcatggttg cgaaactgta cgcctttcac cacctgacca tcacgaacgt
    51601 cgagacacgg gattatgcgt tttgccagca tgcgatggcc tccttcacgg tgaatttacc
    51661 ttccagtaat gcacgaccaa ctattacgcc gcgcacgcca gtgccacgta gggcagcaac
    51721 atcgttaatg tcgccaatgc cgccggagga ctgaaacgcc acttgcggat atctggcaca
    51781 cacttcttca tataaagaga cgttagatcc cgccagcgtg ccgtcgcgcg agatatcggt
    51841 acacaacaca tgtttcaggc cgacgggcag ataggtttcc accagttgtt ccagtgaaac
    51901 gcccgagttc tcttgccagc cgctgactgc cacttgcttg ttgccttgct cgtcaatacg
    51961 gacatccagt gccagcacca gcgcatcggc accgaaacgt tcaaaccagc ctttcactac
    52021 gtccggtgat ttcactgcgg tggaaccgac caccacgcgc gcaacgccag cttccagtaa
    52081 cgccgccacg tcttcttcac tacgaacacc gccgccaacc tgcaccggga cgttaacgcc
    52141 cgcgaccagg gttttaatca gcgggatttg acgcttagcc ggatcttttg ccccggtcag
    52201 atccaccagg tgcaacactt cggcaccttg cgcagcgtaa tcctgtaagc gcggcagcgg
    52261 gtcgttaccg taatcgcgtt gtttgccgta atcgccctga tggagacgca ccacggtgcc
    52321 gtcgattaaa tctaatgccg gaataatcat cacatctcca ggaagttttt cagcaactta
    52381 gcgccagcgg caccagaacg ctccgggtgg aactgcacgc cgtagaagtt atctttttgt
    52441 accgccgcgg tgaacggttc gccgtaatta cactgggcga tggtccacgg attgactggc
    52501 attgcgtagc tgtgaacaaa gtaaaagtac gcgccgtctt caatcccctg aaacaggcgg
    52561 ttgcctgcct gcggataaac gcggttccag cccatatgtg gcagtggcag accaaagtcg
    52621 gtcattttcg gcacgtcttc gtcgatgatg cccagcaagt cgacgccatt gctctcttcg
    52681 ctgtgtcgtc ccaggagttg catccctaag cagatgccca gcaccggttg ggtacaggct
    52741 ttgatgagat caaacagctc gcgctcacgc acctgatcca tcgctgcttg tgcggtgccg
    52801 acgccgggta aaaacagttt atcggccagc aacacaacgt ccgggtcacg gctgactttg
    52861 ggttcataac cgtggcgcgc aatggcagac ttcacagagt tcaggttggc gcagccagta
    52921 tcaaggatca ccacgttcat tacagcactc ctttcgacga gggcagggtg tcgccttcga
    52981 cgcggatggc ctggcgcagg gtacgaccaa aggctttgaa caggctttct acacggtgat
    53041 gatcgttttt acctttggtt ttcaggtgta gtgtcacgcc catggtgtaa gagagcgaac
    53101 ggaagaagtg ttcgatcatt tcagtgctga gatcgcccac acgctggtag gtaaactcgg
    53161 ctttatattc caggtgcggg cgaccagaga tatccagtgc gcagcgggca aggcattcgt
    53221 ccatcggcag cacaaaacca aagcggcaaa taccgcgttt gtcgccgagg gcaattttta
    53281 acgcttcgcc cagcgccagg ccggtatctt cgacggtgtg gtgatcgtcg atatagaggt
    53341 cgcctttgac attgatttcc atgcggaaac cgccgtgggt ggcgatctga tccagcatgt
    53401 gatcaaagaa gccaacgccg gtgttaatct tgctgccacc ttcgcgatcc agccacacct
    53461 ggacgtcaat ctgcgtctct ttggtgttgc gcactacatg ggcgtaacgg tctcgtttag
    53521 tgagttgctc gccaatcatt ggccagttca gggtttcgcg gtcgtagcgt aaaccattaa
    53581 tgcccatgtt ttccgccagt tgaatgtcgg tcgcgcgatc gccaattaca taactgttgg
    53641 cacgatccat cgcttgctca gccagataac cctccaccag ttttactttc ggcttacggc
    53701 agtcgcactc atcggcgggc aggtgcggac aaatcagcac ttcatcaaac tgcacgcctt
    53761 gcgaggtgaa gatctgcatc atcaggttgt gcgggccatc aaaatccgct tgcgggaaac
    53821 tttgtgttcc cagaccatcc tgattagtga tcatcaccag cttgtagccc gctttttgca
    53881 gcttcagcaa ttccgggatc acgcccggtt caaaggcgag tttgtcaaaa cggtccacct
    53941 gaaaatcact cggcggttcg ctaatcaggg ttccatcgcg atcgataaaa agatacttct
    54001 gactcatcaa acttgctccg cacgtaaggc gtcaatgacg cgctggcttt cttcacgggt
    54061 tccgacggta attcgcaggc agccgcttaa agagggttgt ttattctgat cacgtaagat
    54121 aatgccctga tcccacaaag atttaaacac tgcactggag gctttaaagc gcgctagaat
    54181 atagttggtt tcagagtcga aaacctgctc cacgcagggg atctctttca gtgcggcaat
    54241 caggtattcg cgttctgtaa taatttgcgc cacccgttcg cgcatggcga cgattccctg
    54301 cgggcttaac gcctgtgcgg caatgtcggc aaccggcgtc gagagcgggt agggggggat
    54361 cactttcatc agcaggttga tgacttcttc gtttgccagc gtaaatccgc aacgaagccc
    54421 cgccaacgcg aaggctttcg aaagggtacg caaaatcgcc aggtgcggat attccgccag
    54481 ccagccagct aacgatgcct gcgggcaaaa ctcgatataa gcttcatcgg caaccacaat
    54541 cgccttaccg cgggttagct ccagcagggg gcgaaaatcc tgcggattga tcagttgccc
    54601 ggtggggttg ttggggctgc aaacatagac cacttttacg ccgtccagct tgtcggaaat
    54661 gccctgtaag tccagttgcc agttttccag cgtcggcact gtgcggcatt cgacaccaat
    54721 ggtttcagcg ctgacgctgt acatgccgta cgttggcggg cagtagagga tggcgtcttt
    54781 acccggttcg caaaaagcgc ggatcagcag ttcaatacct tcgtccgcgc cacggctgac
    54841 cagcacctgt tccggtttta cgcctgcata ttgcgcgtaa ttttcaatca ccgcttttgg
    54901 ctggcattcc gggtagcggt tgagcgtttg ctgagtaagc tgaaactcca cggctgtggg
    54961 atattcgttg gcgttcagcc agacgtcgcc gttaccgccc agacgacgcg ccgactgata
    55021 cggcgtcagg ttgcggacgt tttcacgcgc taaatcagta atagtcacgg tgctcatgct
    55081 tgctccttaa ggacgttaac acgcaaagta acggcatttt tgtgggcggt caggcgctcg
    55141 gcggcggcca gtgtttcaat ggtcgaagcc agcgcggaga aaccttcttt cgacagttcc
    55201 tgtacggtca tgcgtttctg gaaatctgcc agcccgaggc tggaacaggt ggcggtgtaa
    55261 ccgtaagtcg gcagcacgtg gttggtgccg gaggcgtagt cgcctgccga ttccggtgac
    55321 cagtcaccaa gaaataccga accggcgctg gtgatgccat cgaccagttc gcgggcgttg
    55381 cgggtctgaa tgatcaggtg ctccgggccg tactggttgg agatctccac gcactgcgct
    55441 aaatctttag tcacgatcag gcggctggcg ttcagtgcct ggcgggtggt ttcggcacgc
    55501 ggcagttctg ccagttggcg ttcgacggcc tcggcaacgc gacgcgccat atcagcatcg
    55561 ggggtcagta aaattacctg tgagtcaggg ccgtgttcag cctgagaaag caaatcagaa
    55621 gccacaaaat ccggcgtagc gccgctgtca gcaatcacca gcacttccga cgggcctgcg
    55681 ggcatatcga tcgccgcacc gtccagacgc tggcttacct ggcgttttgc ttcggtgaca
    55741 aaggcgttac ccggcccgaa gattttgtcc actttcggca cagattccgt accaaacgcc
    55801 agcgcggcaa tggcctgtgc gccgccgacg ttaaacacgt cctgcacatc gcacagctgc
    55861 gccacataaa gaatttcatc ggcaatcggc ggcggtgagc acagcaccac ttttttacag
    55921 cccgcaatac gcgccggagt tgccagcatt aataccgttg agaagagcgg ggcggagccg
    55981 cccggaatat acaaacccac tgaagccacc gggcgcgtga cctgctggca acgcacgcct
    56041 ggctgcgttt ctacatctac cggcggcagt ttttgcgcag tgtggaaggt ttcaatactc
    56101 tttactgcca ccgccatcgc ctgttttagc tcgtcgctca ggcgttcgct ggcggcggcg
    56161 atctcatcag cagacacctt cagcgcggta accgtggttt tatcaaactt cgcgctgtat
    56221 tcacgcaggg cctcatcgcc acgcgttttc acgttatcga gaatatcgtt aacggtgcgg
    56281 gtaatgcttt cagaagcgga gatcgccggg cgtattaaca gctggcgttg ttgctccgca
    56341 gtacagctat tccagtcaat gattgtgtta aagctcatgg cgatcactcc atcatcttct
    56401 caatcggcag gaccaggatt gaactggcac ccagtgcttt cagtttttcc atcgtttccc
    56461 agaacagggt ttcgctactc accatgtgca tcgctacgcg ctgttgatca cccgccagcg
    56521 gcagaatggt tgggcgttcg gcacctggca gcagggcgat gacttcatcc aggcgttcgg
    56581 tcggtgcgtg catcatgatg tattttgatt cgcgcgcctg gatcacaccc tgaatacggg
    56641 tcagtagctt gtcgatcagt tgctgtttag attcttccat ttcgccatcg cgctggatca
    56701 ggcaggcttt cgagcggtag ataacttcga cttcgcgcag gccgttagct tccagcgtgg
    56761 caccggtgga aaccagatcg caaatcgcat ccgccagccc ggcacgcggc gcaacttcga
    56821 cagaaccgtt cagtaagcag gatttaaaag agataccttt ctggtcgaga taacgcttga
    56881 gcaggtgagg ataagaggtg gcgatacgtt taccgtttaa ggagagcgga ccgtcccagg
    56941 cttcatcaac cggcgttgcc agcgaaaggc ggcagccgcc gaaatccaga cgacgcaggg
    57001 taaagtagcg cggatcttca ccctgggcgc ggcggttaag cagctcttct tccagcacgt
    57061 tttcgccgat aatcccaagg tctaccacgc cgtccattac cagaccggga atgtcatcgt
    57121 cacgcacgcg cagaatatca atcggcatgt tttctgccat cgcgatcagg cgctgggtgt
    57181 gaagattgat tttaatgcca cagcgcgcca gtaattcgcg tgagtcatca cttaaacggc
    57241 cggatttctg catagctatg cgtaaacgag tgttgtctgt cattttctta ttcctcttta
    57301 aacctgtctg aaccgttaac aaccgcgcaa taaaaaagcc cccggaaggt gatcttccgg
    57361 gggctttctc atgcgttcat gcaccactgg aagatctgaa tgtcttccag cacacatcgc
    57421 ctgaaagact agtcaggatg atggtgatga tggtggtgtt taaattgaac gcgtgtcata
    57481 aaaatctctg tgaatgttta ttcaactgat gtcttttaac ctaaaccact ttcacgttag
    57541 aaagcaaggg cttttttata tattttatgg cgatggattt gtttaatgaa tgagcgggga
    57601 aaggaggggg ctaagaggac tgtcctctaa cttgacagca ccaaaaagag caaagtctga
    57661 tttttcgtct cgctggcaat gtcttgatct acaaactaat taataaatag ttaattaaca
    57721 cctatcattg tacaatgaac tgtacaaaat aggagattta catgcgtaca attagctaca
    57781 gcgaagcgcg tcagaatttg tcggcaacaa tgatgaaagc cgttgaagat catgccccga
    57841 tcctcattac tcgtcagaat ggagaggctt gtgttctgat gtcactcgaa gaatacaact
    57901 cgctggaaga gacggcttat ctactgcgtt cccccgctaa cgcccggaga ttgatggact
    57961 caatcgatag cctgaaatca ggcaaaggaa cggaaaagga cattattgag tgaaactaat
    58021 ctggtctgag gaatcatggg atgattatct gtactggcag gaaacagata agcgaattgt
    58081 taaaaagatc aatgaactta tcaaagatac ccgcagaaca ccatttgaag gtaaggggaa
    58141 accagaaccc ctgaaacata atttgtcagg tttctggtcc cgacgcatta cagaggagca
    58201 ccgtctggta tacgcggtta ctgatgattc actgctcatt gcagcatgtc gttatcatta
    58261 ttgaacagat tcattcatag aatgaattgt cagttttgat acgctagcgt agccttatag
    58321 atctaaggtg tatcaggaga taacggatga aaaaggtcgc aattgtcggg ttagggtggt
    58381 taggtatgcc gctggcgatg tcactttcag cgcgaggctg gcaagtcacc gggagtaaaa
    58441 ccacacaaga tggtgtcgaa gcggcccgaa tgagtggcat tgatagctat ctgcttcgca
    58501 tggagcctga gttagtttgc gattctgacg atctggatgc ccttatggat gccgatgcgc
    58561 tggtcattac gcttccggca cgtcgtagcg gccccggcga tgagttctat ttacaagcgg
    58621 tacaagagtt agtggatagc gcgctggccc atcgtattcc ccgaattatt tttaccagct
    58681 caacatctgt ctatggcgac gcgcaaggca cggttaaaga aactaccccg cgtaatccgg
    58741 taaccaacag tggacgagta ttagaagaac tcgaagactg gctgcacaat ttacccggta
    58801 cttcggtcga tattctgcgt cttgcgggcc tggtcggacc gggacgtcat ccgggacgct
    58861 tctttgctgg aaaaaccgcc cctgatggtg aacatggtgt taatttagtc catttagaag
    58921 atgttattgg cgctatcact ctgttgttac aggcacctaa aggcggacac atctataata
    58981 tatgtgcgcc agctcacact gcgcgtaatg ttttctatcc gcagatggcc cgtttactgg
    59041 ggctgcaacc tccgcagttc agaaatagtc tggacagcgg caaaggtagg attattgatg
    59101 gcagtcggat ttgtaatgaa ctgggatttg aataccagta tcccgatccg ctggtaatgc
    59161 cgctggagta aacgatcacg ccagcaggac gccgcatgca ctgcaagggg gcgtgtatga
    59221 aaccactgct ggatgtgttg atgatcctcg atgccataga aaaagagggc agttttgcgg
    59281 cggcgtcagc caaactctac aagacgccgt ccgctctgag ttacaccgtt cacaagctgg
    59341 aaagcgacct aaatattcaa ttgcttgatc gtagcggaca ccgcgctaaa ttcacccgca
    59401 ccggaaaaat gttattagag aaagggcggg aagttctgca taccgtgcga gaactggaaa
    59461 agcaggcgat caaactgcat gaaggctggg aaaacgagct ggtgattggc gtggacgaca
    59521 cgtttccttt ttctctgctt gcgcctctca ttgaagcttt ttatcaacat catagcgtga
    59581 cgcgcctgaa gtttatcaat ggcgtactcg gtggttcctg ggatgccctg actcaggggc
    59641 gagcggatat tatcgttggc gcgatgcatg agccaccatc ttccagtgag tttggtttct
    59701 cgcggttggg cgatcttgaa caagtctttg cggtcgcgcc tcatcatccg ttagcccagg
    59761 aagaagaacc gttaaaccgt cgtatcatta aacgatatcg ggcgattgtg gtgggggaca
    59821 ctgcgcaggc aggcgcttct acggcctcac aacttctcga cgaacaagaa gccattaccg
    59881 ttttcgattt taaaaccaaa ctggagctgc aaattagcgg ccttggctgc ggctatttac
    59941 cacggtatct ggcgcaacgt tttctcgata gtggcgcgtt aatcgagaag aaagtggtcg
    60001 cccaaactct ctttgaacct gtctggattg gctggaatga acagaccgca gggcttgcca
    60061 gcggctggtg gcgagatgaa attttagcaa atagtgcgat cgccggtgtt tatgcaaaat
    60121 ctgatgacgg aaaatcagcc atttaaagaa aaattattat gacaagcctc tcattctctt
    60181 gtcatttccc ccccatttag gcacaatgcg ccgctgtcaa aaaatgacta aaaaccgacg
    60241 tttaattagc gtcggttatt ttttgcttca aaccaatcat tcataccaag aggccgggct
    60301 tcgtaccgga tagatattta ctaaaaatcg acagttgttg tcgctgagga atccaaaaaa
    60361 atggggcaat tttttgctta cgcgacggtt atcaccgtaa aggagaataa ccatgtcgca
    60421 taacgttact ccaaacacct ctcgcgtgga attgcgtaaa acgcttacgt tagttccggt
    60481 tgtaatgatg ggtctagcct atatgcagcc gatgacgctg tttgatacat ttggtatcgt
    60541 ttcaggcctc acggatggtc atgtgccgac agcctatgcg ttcgcattga ttgcgatcct
    60601 gtttacggct ctgagctacg ggaagctggt tcgccgctat ccttctgctg gctctgcata
    60661 cacttacgcc cagaaatcca ttagcccgac tgtcggcttt atggtgggtt ggtcttctct
    60721 gctcgactat ctgttcgcgc cgatgatcaa cattctgctg gcgaaaatct attttgaagc
    60781 tctggtgcct tccattccat cgtggatgtt tgtggtggcg ctggtggcct ttatgaccgc
    60841 ctttaacctg cgtagtctga aatccgtagc gaacttcaac actgtaatcg tcgtgttgca
    60901 ggtagtgctg atcgcggtga ttctgggcat ggttgtttat ggcgtatttg aaggtgaagg
    60961 cgctggtacg ctggcgagca cgcgtccgtt ctggtctggc gatgcgcatg ttatcccgat
    61021 gattaccggg gcgacaatcc tgtgcttctc ctttaccggc tttgacggca tcagtaatct
    61081 gtcggaagaa accaaagatg cagagcgcgt gatcccgcgt gcgattttcc tgaccgcgct
    61141 gattggcggc atgatcttca tctttgcaac ttacttcctg cagctgtact tcccggatat
    61201 ctctcgcttt aaagatccgg atgcgtcaca gcctgaaatc atgctgtacg ttgcgggtaa
    61261 agcgttccag gttggcgcgc tgatcttctc caccattacc gtactggcgt ccggtatggc
    61321 ggcgcacgca ggcgtagcgc gtctgatgta cgtaatgggt cgtgacggcg tgttcccgaa
    61381 aagcttcttc ggttatgtac acccgaaatg gcgtactccg gcgatgaaca tcgtcctggt
    61441 tggcgcgatt gccctgctgg caatcaactt cgacctggta atggcaacgg cgctgattaa
    61501 ctttggtgcg ctggtggcgt tcacctttgt taacctgtcg gtcatctcgc agttctggat
    61561 ctgtgagaag cgtaacaaga cgctgaaaga tcacttccag tatctgttcc tgccgatgtg
    61621 tggtgcgctg accgttggtg cgctgtgggt taacctggaa gaaagctcaa tggttctggg
    61681 tctgatctgg gctgctatcg gtctgattta cctggcttgc gtcaccaaga gcttccgtaa
    61741 tccggttccg cagtacgaag acgtagcata atcatctttt ctaataataa acaggagtcg
    61801 aatgaacatt tggctccggt ttttttattc ttttttatcg aaatcctcat taataaccat
    61861 ttgttctaat ttagagaatt tggtt
//
LOCUS       DADLSA010000008        56252 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.008, whole genome shotgun sequence.
ACCESSION   DADLSA010000008 DADLSA010000000
VERSION     DADLSA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.008"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..1563)
                     /gene="cirA"
                     /locus_tag="JN691_000519"
     CDS             complement(<1..1563)
                     /gene="cirA"
                     /locus_tag="JN691_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HAY7242045.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGIKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYY"
     gene            complement(1855..3324)
                     /gene="lysP"
                     /locus_tag="JN691_000520"
     CDS             complement(1855..3324)
                     /gene="lysP"
                     /locus_tag="JN691_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAY7242046.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(3530..4411)
                     /locus_tag="JN691_000521"
     CDS             complement(3530..4411)
                     /locus_tag="JN691_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7242047.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGWLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            4510..5559
                     /locus_tag="JN691_000522"
     CDS             4510..5559
                     /locus_tag="JN691_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAY7242048.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSTLKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            5633..6490
                     /gene="nfo"
                     /locus_tag="JN691_000523"
     CDS             5633..6490
                     /gene="nfo"
                     /locus_tag="JN691_000523"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAY7242049.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            6493..7582
                     /locus_tag="JN691_000524"
                     /pseudo
     CDS             6493..7582
                     /locus_tag="JN691_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            complement(7638..8888)
                     /locus_tag="JN691_000525"
     CDS             complement(7638..8888)
                     /locus_tag="JN691_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY7242050.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(8988..9929)
                     /gene="rihB"
                     /locus_tag="JN691_000526"
     CDS             complement(8988..9929)
                     /gene="rihB"
                     /locus_tag="JN691_000526"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAY7242051.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVVGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10060..10758
                     /gene="yeiL"
                     /locus_tag="JN691_000527"
     CDS             10060..10758
                     /gene="yeiL"
                     /locus_tag="JN691_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HAY7242052.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKHLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(10829..12079)
                     /locus_tag="JN691_000528"
     CDS             complement(10829..12079)
                     /locus_tag="JN691_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY7242053.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAILPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12173..13111)
                     /locus_tag="JN691_000529"
     CDS             complement(12173..13111)
                     /locus_tag="JN691_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAY7242054.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(13099..14040)
                     /locus_tag="JN691_000530"
     CDS             complement(13099..14040)
                     /locus_tag="JN691_000530"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAY7242055.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGKNGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(14464..16155)
                     /gene="fruA"
                     /locus_tag="JN691_000531"
     CDS             complement(14464..16155)
                     /gene="fruA"
                     /locus_tag="JN691_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAY7242056.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCADMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(16172..17110)
                     /gene="fruK"
                     /locus_tag="JN691_000532"
     CDS             complement(16172..17110)
                     /gene="fruK"
                     /locus_tag="JN691_000532"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAY7242057.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(17110..18240)
                     /locus_tag="JN691_000533"
     CDS             complement(17110..18240)
                     /locus_tag="JN691_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAY7242058.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            18608..19819
                     /gene="setB"
                     /locus_tag="JN691_000534"
     CDS             18608..19819
                     /gene="setB"
                     /locus_tag="JN691_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAY7242059.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALALALALALALAREHADKTGREAVMFSSFLRA
                     QVSLAWVIGPPLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTV
                     EAPRRNRRDTLLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEI
                     PTMLIAGYFAKRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGG
                     IGMLYFQDLMPGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIA
                     TLFCLLRIKDV"
     gene            complement(19816..20070)
                     /locus_tag="JN691_000535"
     CDS             complement(19816..20070)
                     /locus_tag="JN691_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAY7242060.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            20225..20797
                     /gene="yeiP"
                     /locus_tag="JN691_000536"
     CDS             20225..20797
                     /gene="yeiP"
                     /locus_tag="JN691_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAY7242061.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            21020..22486
                     /locus_tag="JN691_000537"
                     /pseudo
     CDS             21020..22486
                     /locus_tag="JN691_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            22604..23590
                     /locus_tag="JN691_000538"
     CDS             22604..23590
                     /locus_tag="JN691_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY7242062.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            23629..24342
                     /gene="lpxT"
                     /locus_tag="JN691_000539"
     CDS             23629..24342
                     /gene="lpxT"
                     /locus_tag="JN691_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAY7242063.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALTIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            24754..25320
                     /gene="mepS"
                     /locus_tag="JN691_000540"
     CDS             24754..25320
                     /gene="mepS"
                     /locus_tag="JN691_000540"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAY7242064.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            25501..27057
                     /locus_tag="JN691_000541"
     CDS             25501..27057
                     /locus_tag="JN691_000541"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY7242065.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            27139..28953
                     /locus_tag="JN691_000542"
     CDS             27139..28953
                     /locus_tag="JN691_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7242066.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDCLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            28954..30048
                     /locus_tag="JN691_000543"
     CDS             28954..30048
                     /locus_tag="JN691_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAY7242067.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     DNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            30048..31073
                     /locus_tag="JN691_000544"
     CDS             30048..31073
                     /locus_tag="JN691_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7242068.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            31075..32664
                     /gene="yejF"
                     /locus_tag="JN691_000545"
     CDS             31075..32664
                     /gene="yejF"
                     /locus_tag="JN691_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAY7242069.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(32668..33012)
                     /locus_tag="JN691_000546"
     CDS             complement(32668..33012)
                     /locus_tag="JN691_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242070.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(33345..34535)
                     /gene="bcr"
                     /locus_tag="JN691_000547"
     CDS             complement(33345..34535)
                     /gene="bcr"
                     /locus_tag="JN691_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HAY7242071.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(34563..35258)
                     /gene="rsuA"
                     /locus_tag="JN691_000548"
     CDS             complement(34563..35258)
                     /gene="rsuA"
                     /locus_tag="JN691_000548"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAY7242072.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            35408..37168
                     /locus_tag="JN691_000549"
     CDS             35408..37168
                     /locus_tag="JN691_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY7242073.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKENHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            37293..37577
                     /gene="rplY"
                     /locus_tag="JN691_000550"
     CDS             37293..37577
                     /gene="rplY"
                     /locus_tag="JN691_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAY7242074.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(37716..38723)
                     /gene="yejK"
                     /locus_tag="JN691_000551"
     CDS             complement(37716..38723)
                     /gene="yejK"
                     /locus_tag="JN691_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAY7242075.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            38905..39132
                     /locus_tag="JN691_000552"
     CDS             38905..39132
                     /locus_tag="JN691_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAY7242076.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            39152..40912
                     /gene="pbgA"
                     /locus_tag="JN691_000553"
     CDS             39152..40912
                     /gene="pbgA"
                     /locus_tag="JN691_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAY7242077.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            40987..41063
                     /locus_tag="JN691_000554"
     tRNA            40987..41063
                     /locus_tag="JN691_000554"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41021..41023,aa:Pro,seq:ggg)
     gene            complement(41166..43757)
                     /gene="yejO"
                     /locus_tag="JN691_000555"
     CDS             complement(41166..43757)
                     /gene="yejO"
                     /locus_tag="JN691_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HAY7242078.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYDETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGNITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGNMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            44077..44724
                     /gene="narP"
                     /locus_tag="JN691_000556"
     CDS             44077..44724
                     /gene="narP"
                     /locus_tag="JN691_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAY7242079.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     ILHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(44935..45987)
                     /locus_tag="JN691_000557"
     CDS             complement(44935..45987)
                     /locus_tag="JN691_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAY7242080.1"
                     /translation="MRFLLGVLMLMIFGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(45984..46541)
                     /gene="dsbE"
                     /locus_tag="JN691_000558"
     CDS             complement(45984..46541)
                     /gene="dsbE"
                     /locus_tag="JN691_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAY7242081.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(46538..48481)
                     /locus_tag="JN691_000559"
     CDS             complement(46538..48481)
                     /locus_tag="JN691_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY7242082.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFTIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(48478..48957)
                     /gene="ccmE"
                     /locus_tag="JN691_000560"
     CDS             complement(48478..48957)
                     /gene="ccmE"
                     /locus_tag="JN691_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAY7242083.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(48954..49163)
                     /gene="ccmD"
                     /locus_tag="JN691_000561"
     CDS             complement(48954..49163)
                     /gene="ccmD"
                     /locus_tag="JN691_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAY7242084.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(49160..49897)
                     /gene="ccmC"
                     /locus_tag="JN691_000562"
     CDS             complement(49160..49897)
                     /gene="ccmC"
                     /locus_tag="JN691_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAY7242085.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGLA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(49939..50601)
                     /gene="ccmB"
                     /locus_tag="JN691_000563"
     CDS             complement(49939..50601)
                     /gene="ccmB"
                     /locus_tag="JN691_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAY7242086.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(50598..51215)
                     /gene="ccmA"
                     /locus_tag="JN691_000564"
     CDS             complement(50598..51215)
                     /gene="ccmA"
                     /locus_tag="JN691_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAY7242087.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(51234..51836)
                     /gene="napC"
                     /locus_tag="JN691_000565"
     CDS             complement(51234..51836)
                     /gene="napC"
                     /locus_tag="JN691_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAY7242088.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(51846..52295)
                     /gene="napB"
                     /locus_tag="JN691_000566"
     CDS             complement(51846..52295)
                     /gene="napB"
                     /locus_tag="JN691_000566"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAY7242089.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(52292..53155)
                     /gene="napH"
                     /locus_tag="JN691_000567"
     CDS             complement(52292..53155)
                     /gene="napH"
                     /locus_tag="JN691_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAY7242090.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(53142..53837)
                     /gene="napG"
                     /locus_tag="JN691_000568"
     CDS             complement(53142..53837)
                     /gene="napG"
                     /locus_tag="JN691_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAY7242091.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(53844..>56252)
                     /gene="napA"
                     /locus_tag="JN691_000569"
     CDS             complement(53844..>56252)
                     /gene="napA"
                     /locus_tag="JN691_000569"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAY7242092.1"
                     /translation="GVARAVVGQQEAIKWDKAPCRFCGTGCGVLVGTQQGRVVACQGD
                     PDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLLRMKNGKYDKEGEFTPITWDQAFDVME
                     EKFKTALKEKGPESIGMFGSGQWTIWEGYAASKLFKAGFRSNNIDPNARHCMASAVVG
                     FMRTFGMDEPMGCYDDIEQADAFVLWGANMAEMHPILWSRITNRRLSNQNVTVAVLST
                     YQHRSFELADNGIIFTPQSDLVILNYIANYIIQNNAINQDFFSKHVNLRKGATDIGYG
                     LRPTHPLEKAAKNPGSDASEPMSFEDYKAFVAEYTLEKTAEMTGVPKDQLEQLAQLYA
                     DPNKKVISYWTMGFNQHTRGVWANNLVYNLHLLTGKISQPGCGPFSLTGQPSACGTAR
                     EVGTFAHRLPADMVVTNEKHRDICEKKWNIPSGTIPAKIGLHAVAQDRALKDGKLNVY
                     WTMCTNNMQAGPNINEERMPGWRDPRNFIIVSDPYPTVSALAADLILPTAMWVEKEGA
                     YGNAERRTQFWRQQVQAPGEAKSDLWQLVQFSRRFKTEEVWPEELLAKKPELRGKTLY
                     EVLYATPEVSKFPVSELAEDQLNDESRELGFYLQKGLFEEYAWFGRGHGHDLAPFDDY
                     HKARGLRWPVVNGKETQWRYSEGNDPYVKAGEGYKFYGKPDGKAVIFALPFEPAAEAP
                     DEEYDLWLSTGRVLEHWHTGSMTRRVPELHRAFPEAVLFIHPLDAKARDLRRGDKVKV
                     VSRRGEVISIVETRGRNRPPQGLVYMPFFDAAQLVNKLTLDATDPLSKETDFKKCAVK
                     LEKV"
BASE COUNT        13773 a        15206 c        13775 g        13498 t
ORIGIN      
        1 gtagtagcta aataccggta tgcgccgtcc gttagcgccc gtctcaaaac caacaaagtt
       61 ttggtagccc ggtgcagcgt tgacgtcaga cgtacggctg atgctgatac gatctttcac
      121 atcgttacgg aaaacgataa cgctggattc aaccccttcc agccagcctt cttcacccat
      181 gtagtaaagc cccagctccc aactttcgct ggtttctggt ttcagatccg ggctacccac
      241 aatcttacat gcgccacggc aggaattgct cgtccagtca gggctaagtt gcaacagaga
      301 aggtgcttta aatgccgtcg cccagccccc tttcaccgtt acggtgtcgg tggcgttata
      361 aaccaggtag gcacgcggac tccagtgttc accgtaggtt tcgtgatcgt ccatacgcac
      421 gccggtcgtc agcgccagcg gctcgaagat ccgccattca tcttccacaa acagcgcgta
      481 ctggctggca gacgttttgg agctggttcc cccggtcagg ttcaccgcat cgctaagttt
      541 gtcgtgacgc cattcaccgc caaccgtgag aaactgatta atcgccgtca gcggcaacgt
      601 gtatttgccg tcgaccgtat tgctttcgga agttatcggg ctgctgttgc cagggttttt
      661 gttctcgact ttctcaccgt agtatttcag ttcgctggtg ccgtaatccc aacgcccatt
      721 atggctgacg gagtagttct ggcgttccag gcggtttttg tccagcgagt cggaatcacg
      781 atcctgacgg tcgaaaccgt atccggcagt aaaatcgtga ttttgattcg gtgtccaggc
      841 aaattcgaca ttgccgtcgc ggctggagaa tccttcaata cgcggcgttt ctccggtatc
      901 ggtggtcgtt gagttttgcg ggtcatcctt ttcacgtttt gccaggctgc cgtaagcttt
      961 tattcccagc acaccatcaa ttaatggtcc actggtaaag aactgaccgt tataggtatc
     1021 accgcgatcg cgatgttcct gaatggtggt atcgacggta acggtacccg accatttctg
     1081 accgattttt ttggtgatga tattcactac accgccgagc gcatcggagc cgtatagcga
     1141 cgacatcggg ccacgaacca cttcaatacg ttcgatggaa tcgaccggga tccagttcag
     1201 atcgaaatca ttgtggcgga agacggcatt gcgggagtta acgcgtttac cgtcgacaag
     1261 aatcagggtg tagctgctgt ccagaccacg aatactaacg cccttacggt tatccccttc
     1321 gttcgtcagt tgtacgccag gcacttcttt gaggacatcc ttcagattct gtaccggttt
     1381 tcgctgcagg tcttcctggg taatgacgct gatactggcg ggtgcatctt taagattttg
     1441 ttccacggaa gatgcagtga caaccatcgt ttcgccatca tcatcgaccg ctaacacagg
     1501 ccatgcacaa gaaatagcgg acaaacacag cccgacccgt acgaaagggt tcaacctaaa
     1561 cattccatat ctccatgagg taactacgaa aataaaatgg gtatcgctca catcttcttt
     1621 acgtcccctt gcgtacggca gcatcacggt ggacttattt gtatgcaggt gatcatccca
     1681 gaaagccgga acagcctctg atttgtcagc cttttttgat tgcgtcgtaa cgataatgca
     1741 aacgataaca attatcaatc caaatgttaa attttatatc cgcatgattt gtcagggaaa
     1801 tggaattgaa aaagccctct cggttgagag ggcttagcaa ggaagggagg aaacttattt
     1861 cttatcgttc tgcgggaact tcatttcgct gtagcgtacg aagtgagttc ctttaatcag
     1921 cttgtagccg aaccaaataa tcaggaacag cgggatacca atatacgttg ccgctacgcc
     1981 gccccagtca atggtatctt tcaggaacgc ttcgtagttc tggcccaaag tgataatcag
     2041 acacagaatg aatgcgaaga tcggccccag tgggaagaaa cctgaacggt acggcagatc
     2101 gttaatgtcg tgtccctgca atacgtaacc gcgacggaag cgatagtggc taatggcaat
     2161 ccccagccag gcgataaaac ccgtcatccc ggaggtgttc agcagccaca ggtataccgt
     2221 ctggttgcca aacatggagg tcaggaagca caaaccggca atcaccgtcg tcgcatacag
     2281 cgcattacgc ggcacgccac cacgcgacaa tttagcgaaa atgcgcggcg ctttaccgtc
     2341 acacgccagg gtgtacagca tacgagtaga cgcatacata ccggagttac ctgctgacag
     2401 caccgccgtc agaataactg cgttcatcac cgccgccgca gagagcagac ccgcgtgctg
     2461 gaacaccagg gtgaacggac tgacgctgat gtctttaaca tcgttacgca gcaggctcgg
     2521 atcggtatac ggaataatca ggctgataat caggatcgcg aacacataga acaacaggat
     2581 tcgccagaat acctgacgta ccgcgcgtgg aatgtttttc gccggatctt cggactcgcc
     2641 tgcagcaata ccgatcagct cggttccctg gaaagagaag ccgacaatca tagctacgcc
     2701 gatcatcgcc gcaaaaccac cagcaaacgg cgcttcgccg attgtccagt tgctccagcc
     2761 cgcaggctgc gcgcctttga agataccgat aatcatcagc acgccaacga tgataaagac
     2821 aataactgtc gtgactttga tcagtgagaa ccagtattcc gcttcaccaa agccgcgaac
     2881 agagatgtag ttcaacagga agataacgcc gaggaacaac gcactccaga tccagcccgg
     2941 tgtatccggg aaccaccagc tcatgaccag ctgtgctgca accaggtcaa cggcaatagt
     3001 caccgcccag ttgtaccagt agttccagcc cagcgcgaag ccaaagcctt cttcaacata
     3061 gttctgaccg taagtggcaa acgaaccgga aaccggcata tatgcagcca attcaccgag
     3121 actggtcatc aggaagtaaa ccatcagtcc aatcagcata tacgagagca atgccccgcc
     3181 cggacctgcc tgagaaatcg ttgcgccaga ggcaacaaaa agacctgtac cgatggaacc
     3241 gccaatggca atcatcgtca ggtgacgcgc ctttaattca cggcgtaagc ccggcgcttc
     3301 tgtagtttta gtttcggaac ccatacaaaa atgctatcca tcttaaaaat gatggcgcga
     3361 ttgtagcaga cgattggcaa tccttccggc agaaatacac ggttataaga caccttcatg
     3421 atcgcccagg gattataagt aaagcagcca atctcttttc tggcgaatgc atgctgaagg
     3481 cgctgcgtca cgaaatcgac acagcaccag catgttcttg tacagcaact taacgcggca
     3541 catttgcggg atcgcaatag tccagaaagc gccgtagcgc gttggaaagg tgtttttgcc
     3601 gatgatgtat acgccacaac gtacgcatca ggcgcggcag agggaccgca acttcactta
     3661 atgtgcctgc ctgcaattga tcttcaatca cacgtcgcga caggcaacta attcccaacc
     3721 catgacgcac cgcatgtttg attgcctcgg agttacctaa ttccatcgcc atctcaaact
     3781 tcggtaaatg tgacagcaac agataatcga caatctcccg cgtgccggaa ccgcgttcac
     3841 gcaggatcca cggcgcagcg gccagctgtt ctaaggtgac cggaccacga gccaacggcg
     3901 aagtcggtgc ggcgaaaacc accagctcgt cttccagcca cggttcagaa atgatttcag
     3961 tgctgtggca cggtccttca ataaagccaa tatcaacgcg gaaatccagc accgcttgca
     4021 tcacgtcctg gctgttccca acgctaagtt caatcggcaa ctgcggataa tcatggcgat
     4081 aacgggcgat aactgcaggc agaatgtagt taccgatggt actactggca tagatacgaa
     4141 tcgcgccgtt gtcttcgcga aacagttgtt cgatttcaac cgcctgttca agcagtgcca
     4201 acgctcgcgg atagagcagc cacccgtgtt cattaacaac cagtcttttc cccacgcgat
     4261 caaacagttg aacgccaagc tgcccttcca ggtcagtcaa ggctgcactc actgctgatt
     4321 gcgacaacgc cagcatcacc gacgcctggg tggttgatcc acttttcaat acttctgcaa
     4381 aaacttccaa ctgccggagg gtgatgtgca tagtcgctta ccacttataa agattaatta
     4441 taaatatata atcaatttta tttttaaacc agttagtcgt aaccttatgc ccggttaaag
     4501 gagagggtta tgacgaatat caccttacag aaacaacatc gtacactgtg gcattttatt
     4561 ccggggttag ccctgagtgc agttatcacc ggggtcgccc tgtggggtgg ttccattccc
     4621 gcggttgcgg gtgccgggtt tagtgccctc accctcgcaa tcttgttggg gatggtttta
     4681 ggcaacacca tctatccgca catctggaaa agctgtgacg gtggtgtgct gtttgccaag
     4741 caatatttat tacgtctggg tatcattctt tatggcttcc gtctgacgtt ctcgcaaatt
     4801 gccgatgtcg gtatcagtgg gatcatcatt gacgtgctga cgctgtccag taccttcctg
     4861 ctggcttgct tcctggggca gaaagtgttt ggtctggata agcacaccag ttggttgatc
     4921 ggtgccggta gcagtatctg tggtgctgcc gcggtactgg cgactgagcc ggtagtgaaa
     4981 gcggaagcca gtaaagtaac cgtggctgtt gcaaccgttg ttatcttcgg gaccgtcgcg
     5041 attttcctct acccggcgat atatccgctg atgtctcaat ggtttagtcc ggaaactttc
     5101 ggtatctaca ttggttctac tgtgcacgaa gtggcgcagg tggtggcggc aggtcatgcc
     5161 atcagcccgg atgcagaaaa cgcagcagtt atttccaaaa tgctgcgcgt gatgatgctg
     5221 gctccgttcc tcatcctgct ggcggcgcgt gttaaacagc tgtctggggc gaacagcggc
     5281 gagaaaagca aaatcactat tccgtggttt gccatcttgt tcatcgtagt tgccatcttt
     5341 aactcgttcc acctgttacc gcagagcgtg gtgaacatgc tggtaacgct ggataccttc
     5401 ctgctggcaa tggcgatggc ggcgctgggt ctgaccactc acgtcagcac gctgaaaaaa
     5461 gctggggcga aacctctgct gatggcactg gtactgtttg cctggctgat tgttggtggt
     5521 ggtgcgatta actatgtgat tcaaagcgtc atcgcataaa ccactacttc ttgctcctgt
     5581 taacccgcta tcattaccgt tttcctccag cgggtttaac aggagtcctc gcatgaaata
     5641 cattggagcg cacgttagtg ctgctggcgg tctggcaaat gccgcaattc gcgccgccga
     5701 aatcgacgca accgcgtttg ccttgttcac caaaaaccaa cgtcagtggc gtgccgcacc
     5761 gctcacgacg caaaccatcg atgaattcaa agccgcctgt gaaaaatatc actacacatc
     5821 ggcgcaaatt cttccccacg acagttatct gatcaacctc ggacatccgg tcactgaagc
     5881 tctggaaaaa tcgcgcgatg cctttataga tgaaatgcag cgttgcgaac aactggggct
     5941 ttctttgctc aacttccacc ctggcagcca tctgatgcag atttcagaag aggattgcct
     6001 tgcgcgtatt gccgaatcca tcaacattgc gctggataaa actcaaggtg taacagcggt
     6061 gattgaaaac accgccggtc agggcagtaa cttagggttt aaattcgaac atctcgcggc
     6121 gattatcgac ggcgtggaag ataaatcccg cgtcggcgtc tgcattgata cctgccatgc
     6181 tttcgctgcc gggtatgatt tgcgtactcc agccgaatgc gaaaaaacgt tcgctgattt
     6241 tgcccgtatt gtcggcttta agtatctgcg cgggatgcac cttaacgatg cgaaaagtac
     6301 ctttggcagc cgcgttgacc gccatcatag cctcggtgaa ggcaatatcg gtcatgatgc
     6361 gttccgctgg attatgcaga acgaccgttt cgacggcatt ccgctgatcc ttgaaaccat
     6421 caacccggat atctgggcag aagagatcgc ctggctgaaa gcgcaacaaa ctgaaaaagc
     6481 ggtagcctga agatgaataa ccgggaaaag gagatccttg caattttacg gcgtaacccg
     6541 ctgattcagc agaacgaaat tgcggacatg ctgcaaatca gtcgttcgcg cgttgcggcg
     6601 catattatgg atttaatgcg caaaggccgg attaaaggca aaggttacat tctcaccgag
     6661 caggaatact gcgtagtggt ggggacaatc aatatggata ttcgcgggat ggcggatatc
     6721 cattacccgc aagcggcttc tcatcccggt acaattcatt gctcggcagg cggcgttgga
     6781 cgcaatatcg cccacaatct ggcgctgtta gcgccgtgac gtccatttgc tttcagtgat
     6841 tggcgatgac ttttatggcg aaatgctcct agaagaaacg cgccgtgccg gcgtgaatgt
     6901 ctccggctgc gttcgtttgc atggtcaaag cacatcgacg tatctggcaa ttgccaatcg
     6961 agacgatcaa accgtgctgg cgattaacga tacccatctg ctggaacagt tgacaccgca
     7021 actactgaac gggtcgcgcg atttacttcg tcatgcgggc gtggtactgg ctgattgcaa
     7081 cctgacagcc gaggcgctgg aatgggtctt taccctcgct gatgagatcc cggtgtttgt
     7141 cgataccgtt tcagaattca aagcgggcaa aatcaaacac tggctggcgc atattcacac
     7201 cctgaaaccc actttaccgg agctggaaat tttatgggga caggcgatca ccagcgatgc
     7261 tgaccgtaat gccgcagtga atgcattgca tcagcaaggt gttcagcaac tgtttgttta
     7321 tttgcccgat gagtcagttt atttcagcga aaaggatggt gaacaatttt tgctgactgt
     7381 gccagcgcat acgacggtag acagttttgg tgctgacgat ggttttatgg cgggtctggt
     7441 atatagcttt ctggagggaa acaatttccg tgacagcgcc cgttttgcga tggcctgcgc
     7501 ggcaatttca cgcgccagcg gcagcttaaa caaccctacc ctgtctgccg ataacgcgct
     7561 gtcattagtg ccaatggtgt aacaatgttg ccggattcga cgcttgaccg catctaatcc
     7621 ggctcataaa ttgcacacta cgctaaacca ataaagaacc cggcaatggt ggcactcatc
     7681 agattagaaa gtgtcgccgc cgccagcgcg cgtaaaccaa gctgggcgat ttccggcgca
     7741 cggtgtggcg caaccgcaga aaacgccccc accaccaccc cgatagaacc aaagttagcg
     7801 aaaccgcaca acgcgaagga aataatcgcc acggttttag catccagagt gccagccgtt
     7861 tgcagatagg gtgagaaatt gagataagcg acaaattcat ttattgccag tttctgtcca
     7921 atcaaactcc cggcaagatt tgcatcactc cagtcaacac ccattaccca cgcaagtggg
     7981 gccagcaggt aacctaaaat ggactccagc gaggcatgtt caaaaccaaa ccagccgcca
     8041 acgccgccga taataccgtt aattaacgca atgatggcga caaatgccat aacaactgtc
     8101 gctacaccgg cggcgatttt cagcccggtc attgcccctg tcgcagcggc ttcaataatg
     8161 ctttttggcg gtgtttcggt gaaagagagg ttattaaaag aaacctgcga agattccgta
     8221 gccgggctta acaggcgggc aaacaagatc ccgccaggga tcgccattaa cgatgccgcc
     8281 agcaaatatt ccacaggtac gcccagtgcg gcgtaaccaa tcattgtcga accagcaatc
     8341 gaggccatgc cactacaaat cgctgtaaat aattcattgc gattcagacg atcgataaag
     8401 ggtttgacga tcgccggaat ttcgttttgc ccgaggaaaa tggtggtgac cgcgacgaat
     8461 gactcgatct tgctgatatt taaagctttc tggaagatac cgccgagaat gcgaattaag
     8521 atccccatca caccgatgta gtagagaata ctcaccagtg cagtgacgaa gataattgcc
     8581 ggtaataccc tgaaaccaaa gataaatcct gcgccatcaa ataaggtatc cattttcggt
     8641 ccgaccagag aaccgaagat aaatgcgcta cctgcgtcgc tgtacgccat cactttgtgg
     8701 acgccaaaag cgactttttc tgcgacccaa cgccctggcg gtaaccaaag cataatcccg
     8761 ccaatcacga cctgtaacac taacgccgcg ccaacggtac gcaggctgat cttcttcttg
     8821 tttactgaca gtaaaaacgc aattgccagc agtaccacca ttcccagaac acttctcatg
     8881 acatccatag tgattatctc ttcatgtcat tttgggcgac ggctttctgc ccatcagaaa
     8941 tagccgggcc tgcatcttgc gatactgtcc cggcttgtta tcaacactta atgggttttg
     9001 atgtagccgc gcacgcactc ttcgactaag ccccagaacc agtcggtatc aatagtgatg
     9061 ccgactttgg tattagcggg cttgccaaga acgcccagtt cgtcgcagac cgtacgcccg
     9121 tagcaagggc cgctgttgac gtccacttcg acgtacatat cctgggtttt aatgccatca
     9181 gggttgatca gataaccaat gcaggtggcg tcatgcaccg ggccgccagc caggccgtag
     9241 tattcgaact gcgttttgag agtgaagttc atgatgtcgc tgaacagctc tccggcaggg
     9301 ccgcctaccc tttccatccg agcaattaca tccggggtgc aaacggtctg gttggtaaga
     9361 tcgaggccca tcatcactaa tggaacgccg gaggtgaaca ccacgcgggc ggcttccggg
     9421 tcggcaaaga tattgaactc ggcagatggg gtgaagttgc ctgtaccgta agcgccgccc
     9481 attagcacaa tttcacggat tttgggcagg attgcgggtt gcatacgcat tgccaccgcg
     9541 atatttgaaa gcggaccaac cggcaccaga gtgatatcgc catcgcttgc catcagggta
     9601 tcgatgatat atttcaccgc atgggtactt tctgcctggc gggtcagcgg ctcgaatacc
     9661 gggccatcca gtccggtttc accgtgaata ttatcggcaa cgatttgttt acgcataatg
     9721 ggttgcggca tccccgcata aaccggaaca ttaatctcca gtttctggca aacattcagg
     9781 ccattaatta atgttttatc aagcgtctga ttacccacta caatagtgat gcctaataaa
     9841 tctattgccg gatgtttcgc tgccatcatc attgcaatag catcatcatg acccggatca
     9901 caatccagaa taatttttct cttttccatt gtttatttcc tctgtttcca gttgcgttat
     9961 ttttttctac agcaaagaaa attcgaccgg gccgatgatt gaatcttaac aacagcgtac
    10021 gtatgctaaa tatgagaaat ctcatagcgg ataaacatcg tgaaagaaat ccacaataat
    10081 gaccttaagc agcaattgat gagtgaatct gcgtttaagg attgcttttc aacggatgtt
    10141 tcagccgata cgcggctgtt tcatttttta gcgcgtgact acattgtgca ggaagggcaa
    10201 cagccgtcat ggctgtttta tctgacgcga ggccgcgcca ggctttacgc cacgcttgct
    10261 aatggtcgcg tgtcgctgat cgatttcttt gccgctccct gttttattgg cgagattgag
    10321 ttaatcgaca aagaccatga accgcgtgcg gtgcaggcta ttgaagagtg ttggtgcctt
    10381 gcgctcccta tgaaacatta ccgtccgctg ttattaaacg acaccctatt tttacgaaaa
    10441 ctttgcgtca ccttaagtca taaaaattat cgtaatattg tttctttaac tcagaatcaa
    10501 tcatttccgt tagtgaatcg cctggcagca tttatattac tctcacaaga aggtgatctt
    10561 tatcacgaaa agcatacgca agcggcagag tatttaggcg tttcttatcg acatctttta
    10621 tatgttctcg cgcaattcat tcacgacggt ttattaatta aaagcaagaa aggatatcta
    10681 attaaaaaca gaaagcattt gtcaggactg gcgctggaga tggacccgga gaataaattc
    10741 tccgggatga tgcagtaaaa attatttgca atagcgcgat tgccggatgc aacgcttaat
    10801 acgttttatc cggtctacaa atcgagcatt acgccagacc aataaagaac ccggcaatag
    10861 tcgcactcat caggttggaa agcgttgctg ctgccagcgc ccgtaaacca agctgggcga
    10921 tttctggagc acgttttggc aaaatagccg aaaatgcgcc aacgacaaca ccgatagaac
    10981 caaagttagc aaaaccacaa agcgcaaagg atataatcgc cacggttttc acttccagcg
    11041 tgccgcccgt ttgcaggtat ggggagaaac tcaggtaagc gacgaattcg ttaatcgcca
    11101 gtttctgccc aatcaggcta ccagcaaggt tggcatcact ccagtccaca cccatgatcc
    11161 acgccagcgg tgccagcaca tagccaaaaa tactttccag agaggcattg gcgaaaccaa
    11221 accagccgcc aattccgccg ataatgccgt tgatcagcgc aataattgcg acaaacgcca
    11281 ttaccaccgt cgctacacca gcggcgattt tcagcccggt catcgcaccg ctcgccgccg
    11341 cttcgataaa gctttttggc ggcgtttcgc tgaacgacag attttcaaat gtgacctgcg
    11401 aaggctcggt tgccgggcta agaatacgtg caaacaaaat accgccgggg atcgccatca
    11461 gcgatgccgc taacaggtag tcaattggta cgcccattcc ggcataacca atcatcatcg
    11521 aaccagcaat ggacgccatc ccgctacaaa ttgcggtaaa caactcgttg cgattcatgc
    11581 gatcgataaa cggtttaacg atcgccggga tctcattttg cccgaggaaa atagtggtaa
    11641 ccgcaacaaa agattcgatt ttgctgatgt tgagggcttt ctggaaaatg ctgccaagga
    11701 tgcgaatcag cagccccatc acgccaatgt agtacagcag actgatgagc gcagtaacga
    11761 aaataatcgc cggaagtacg cgaaaggcga agataaaacc cgcaccgtca aacaggacat
    11821 ccattttcgg cccaaccagc gaaccaaaaa tgaaggcgct accggcatca ctgtaagaca
    11881 tcactttatg aacgcctaat gccgcctgtt ctactgccca ttttcccggt gggaagtaga
    11941 gcatgatgcc accaatagcg atttgcagca gcagtgcggc tccaaccgtg cgcaaactga
    12001 tgctcttttt attcactgac aacagaaatg ctattgccag taacaccacc atccccacaa
    12061 cacttctcat tatatccata atgattttcc cttcatgcca gtaaacccgg cgtcagcgcc
    12121 aggttttggt atgcttgatg agtacgggcg acggctttct gcccgtcaga aattaacccg
    12181 cgagacgctg gtattctttg gcaatttcac tcgccagaat ggcgttgttg aacaccagct
    12241 ggatattgga tttcaggctg tcaccgccgg tcagctcagc aacgcgcgcc agcaggaacg
    12301 gtgtactttc tttaccaata acgccctgct cttcggcttc ggcgacggct tgatctatgg
    12361 ctgcattgat agattcctcc ggcatcgcaa actgttccgg gatcgggttc gctaccacga
    12421 ggccaccgtt cagcccgctt tgccatttca ccgccattgc acgggcaatt tcactggcgc
    12481 tgtcgagacg aatgctgacg tcaaacgagc tggtacggca gaaaaacgca ggcagcgctt
    12541 tagtctgata gccaattaac ggcacaccga aggtttctaa atactcagtg gttaatccta
    12601 aatcgagaat agatttcgcc ccggcacaaa caacggtgac attagtattt gccagttctt
    12661 gcaaatcggc agaaatatcg aaggtatgtt ccgccccgcg atgcacacca ccaattcccc
    12721 cggtggcaaa tactttaatt ccggcaagcg ccgcaataat catcgttgaa gccacagtgg
    12781 ttgcgccatt ttttccggcg gcaacaacaa aaggtaaatc gcgacgacta actttggtca
    12841 cgttatgccc ttcacgaccc agtaattcaa ttttttcttt gcttaaaccc actttcatca
    12901 cgccgccaat aatggcgata gtggcaggta ctgcgccctg tttacgaata gtttcttcta
    12961 cttcaattgc ggtctgggca ttttgtggga acggcatccc gtgagaaata atggtcgatt
    13021 ccagcgccac aaccggtttt ttgtttttta aagcgtcctg cacttccggg gaaatttgta
    13081 ataattcagg ggaaattttt aattcagaca ttctgcgttc tccactaacg atataacgtt
    13141 ggcaatcgat aaatcggggt tattggtgta ttcacaggag agcgccattg acgaacatcc
    13201 ctgtgcgaaa cgaacagatt cggcaaacgg cattccgtct acccaacacg aagcaagtcc
    13261 cgccatcatg gcatcgcccg ctccggtaac attaataaca ttggttttga tcggcgcaga
    13321 ccagccattt ttaccgctga tatcgctgta ataaacgccg tcgccgccca tgctcaatac
    13381 cagtcggttc aggccatgtt gatggaacca ggcagcaact tttgccacat cgtcacgccc
    13441 tgacagcgca atcccactca gggtttccgc ttcaaggcgg tttggcttca gagtgtggat
    13501 ctgatttagg cggtcgcgca ctttgacaca tttccacgct gaaaccggat cgacaaatac
    13561 gggtacgttg gcggcattat ccagaatcca tgccagtgcc tcttcactga tattacagtc
    13621 cgcgacaatg acctttgccc tctgaataaa ttcacggtgc tgtgcgagat attcagctgt
    13681 aatagcgttg ctaatattca tgtcatttat agcaaccagc atttcaccgg tattatcgag
    13741 taatgataaa taactcgacg tattttctcc cggcacaatc aggcatttat cgacataaac
    13801 accagattga ttggtttgcg ttagcagcga ttgaccataa aaatcactgc ctacggcgct
    13861 cagtagccag gctttgttac ccagcaacgc caggttttgt gcaatattgc gccctactcc
    13921 accaggcgta aattttattt tacctggatt tgaatccgca taatttaatg attcatgtga
    13981 atatccggcg acatcaatat tcgccgaacc tataattacg acataatcct tttcgcgcat
    14041 agcacgtcct ctggtaaaaa attaccaacc ctgtcaatcc acaagaatat agatactgca
    14101 aagacggttt aaacatgtgt tcatatttaa acatgtgctc atagtatgca tgttttacgt
    14161 aaagtaaatt accgccagag aggcaaaatg ggcagatttg agcaggctca caaaatgcga
    14221 ttaaatgcct gaattacaaa agcgaggaat ttgtgacggg atgcacagaa caggaacact
    14281 aaaaaagggc agaaaataat ctgcccttga ggaaataacc ttagtgtttg atgaacgttt
    14341 gaagtttcta ctgaaagata aaaccattta aattcataaa attacattca caagctggtc
    14401 attctctttg cgctcatcaa atgttacagg gcaggaaatt tctgccctgt aacacacctt
    14461 ttattacgct gctttcgcta ctgcgtccac ttccggacgt ttcaggaaag cataggccaa
    14521 acccgccacc agcgtaccgg caataattgc taccaggtaa cccaataccg gcgtaatagc
    14581 gccagggatc agcagaacaa acagaccacc gtgcggtgcc atcagtttcg caccaatcgc
    14641 cattgagatt gcgccagtca gcgccccacc cacgatacag cacggcagca cacgcatcgg
    14701 atcacgagca gcaaacggaa ttgcaccttc cgaaatgaag cacagtccca ataccagagc
    14761 ggctttgcca ccttcctgct gcgctttgtc gaatttgcga cgcgccacca ttgttgccag
    14821 gcccattgcc agcggtggca ccatacctgc cgccataatc gccgccatcg ggccataggt
    14881 ttgagtactc agcagaccca caccgaatgc gtacgctgct ttgtttaccg gaccgcccat
    14941 gtcagcacac atcatgccac cgaggatcgc ccccagcaga accgcattcg cagtccccat
    15001 ggtctgcagc cagtgagtca gcccttcgag aatgccagca actggtttac cgatcaggta
    15061 gatcatcgcc agaccgacca ccagactgga aattagcgga atgatcagga tcggtttcag
    15121 cgcctccata ctctgcggca gcttcagttg cgtactgatt aacttcgcaa tgtaaccagc
    15181 caggaagccc gcaataatac cgccaatgaa gccagaaccg gtgctgaccg ccagcatacc
    15241 gccaatcaga cccggcgtga ggcccggacg atcggcaatg gagaaggcaa tataacctgc
    15301 cagtaccggc accatcagcg caaaggctga accaccacca atctgcatca gcgccgctgc
    15361 caacgtaccc ggctctttaa acgcttcgat accaaaagca aaagaaagcg cgatacacag
    15421 accaccagca acgaccatcg gcagcatgta agagacgccc gttagcaagt gacgataagc
    15481 gcctgcactc tctttcttac cttcagtggt cgccgtttga gctttgcccg ccggttcata
    15541 cggcgttgct tcagcaaccg ctttatccag ttcctgcgcg gttttcttca gcgccagacc
    15601 ggtagaggta cgatacatcg gtttaccagc aaatttcgcc agatccactt cgatatctgc
    15661 cgccacaatc accagatccg ctgcggctac ttcctcggga gtgattgcat tacccgcgcc
    15721 aacagaacca cgggtttcaa ctttcaccca ccagccacgt tttttcgctt cggtttcaat
    15781 ggcttcagcc gccataaagg tgtgtgctac gccagtcggg caagcagtca ccgcaactac
    15841 gcgtttcgga ccgctggcgg caaccggtgc tgtcgcagtg accggcgcag tgtaaggttt
    15901 cgcatggcct ttggcttcac tcaggaacag ctcagggtgc gcaactgccc gggaaatatc
    15961 acccagccag acatttttac cgttcagcgc gctgtcattc gggatggaat caccgagaac
    16021 aatcgccatt tctgcgtcgt tcggattgtc gatgatttcc agttttgctt ttcgcgccgc
    16081 cgcgcccagc agggtcttcg ccatataggc gcgtgcctga ccgagattag cgtcaataat
    16141 cagcagcgtt ttcattatgc ctctcctgct gtcagttaaa aggttgtaag tcgacgcgcg
    16201 ccatcattgc ggccaactgc ggacgatcgg taatacccac attgctttga cttaccgcca
    16261 gggctgcaac agctgtcgcc agacgcagtg tgtgttcact ggattcacgc atcagcaagc
    16321 cataaatcag gccaccaacc atagaatccc ctgcgccaac ggtgcttacg acatcgactg
    16381 acggtggttt agcgatccat tcgccggagg cattaaccca aagcgcgcct tcggcaccca
    16441 gtgaaataac aacatgcgca ataccttgtt cacgtagcgc atgtgcagct tcaatcacat
    16501 ctttcatttc aggcagttta cggcctgccc agatttccaa ctcgcggcgg ttaggtttca
    16561 ccagccacgg tgccgctttc aaacctgcta ctaacgcttc acggctacta tcaaagataa
    16621 tgcaaggaca ctgactacgc aggcgagtca tccagtcggt gaacgcttcc gggctgacgc
    16681 ctgacggtaa gcttccgctg acacagacca tatcgaactg accgagccag ctcagagaat
    16741 cagtcacaaa gcgttcccag tcggcggggg tgacttcaaa acccgagaag ttgaagtcgg
    16801 tcacttcgcc gtctttttcc gtcagcttaa cgttaattcg ggtgcgcccc tgtacaacct
    16861 ggaaacggtt ggcaatgccc agctcgctga acagttgctg aaaaccatcc tgattgtctt
    16921 tacccaggaa gccgccaacg gtgacatcaa ttcccaggtc ttttaatact ttggccacgt
    16981 tgatgccttt acccgccgca tgcagaccgg tggttttcac caggttcact tcgccgcgtt
    17041 caatttccgg gcagaaacca acaaggtcat aagccggatt aagggtgatc gtagcaacac
    17101 gtctgctcat tatgcgccct ccccaagacc agcagcgata gcgtcgccaa tggctttcag
    17161 cgcctgttca gcatctgcac cctgggcggt aaagcgtagg cgatgacctt tcttaacgcc
    17221 aagtgccaca actttcatca gactacgtcc gtttgccggt ttgccggtgc catcaaggtt
    17281 tgtcacggta atatcactgt taaattgttt aatggtattg accagcatgg tacctggacg
    17341 agcatgcagg ccgtgttcat tgcgcaccac aaactccgcg cttaacacgt cgtcggtcgg
    17401 cgcatcatcg ctggtcagca gcgccagcaa cgttgccgca tccgctttca gcaagcggtc
    17461 agctttattg tcgagcaaca aatcagcgag acgcttaaga accgcgatgg gctgatcgtc
    17521 attcatcgcc acactcacca gcatggctgc cgtttcgccg tccacatcaa aagcatttgc
    17581 cgcacggctt accgcaatcg cactacgcag attgccttcg gcgctatcgc tcagccagat
    17641 accctgtccg agattcagcg gttgttcatt gatggctttg gtgacgaaag tggcgtcaac
    17701 tgccctcgcc tctttcagac gcgcagcgtt cagcgcctga agagtcagca gatcgctggc
    17761 gacgatatcc agcgtcagca tttcgttgtc gagcttcagc tgctcactct gcttttcgcc
    17821 catcagtaat gcgcgaagtt cttctgctgt tgttgctgac ttcagttgtt cagcaacgga
    17881 atcatcgctc agtacgtggg tcagctggcg tagcaggccc agatgtttat ccgagctggc
    17941 agcaataccg attgccacgt acgctacctg accgtcaccc caggtgacgc cttccgggaa
    18001 ctgaaatacc tgaacgccgg ttttcagcac ctgatcgcgg gtgtcggtag tgccgtgtgg
    18061 aatagcaata ccattgccga ggaacgttga ggtttgctgt tcgcgcgcca gcatgccatt
    18121 gacgtagcct tctgctacat taccggcctg caccagcgcc gcagcgacct ggcgaatcgc
    18181 ctcttctttg tctccggcct tttcgcccgg atggatgtcc tgtacggata actggaacat
    18241 agttctcctc tcttgctgaa ttgaaacgat tcagcctcta tgagaaaaaa agcgccaacc
    18301 tggcttaggg ttaaagacaa gatcgcgctg aaacgtttca agaaagcata atacttctgt
    18361 ttcagcacgc aaggaaagcc gtaaagttat gagcaaaagt ttgatgtgct gcacaatttt
    18421 ttgtcatttt tcctaattgc tgatgggaaa atccgttatc agcaatttca tttcagcagc
    18481 gtcagcagcc aatcagcaac agtgaaatgg cattttgatt attcgtggcg cttttgattt
    18541 cgctgtctgt ttattttctg acaagcagcg taaactccgc gtcttcctct tccagtgatc
    18601 gaccagcatg cataactccc ccgcagtctc cagcgcgaaa tcgtttgacc tgacctcgac
    18661 ggcgttttta atcgttgcct ttctcaccgg tattgcgggt gctctgcaaa ccccgacact
    18721 cagtattttc cttaccgatg aagtacatgc ccgtccggcg atggtgggat tcttctttac
    18781 cggcagcgct gtcattggga ttctggtaag tcagtttctc gctgggcgct ctgataagcg
    18841 cggcgatcgc aaatcgctga ttgtcttttg ctgcctgtta ggcgtgctgg cctgcaccct
    18901 ttttgcctgg aatcgcaact actttgtttt gctattcgtt ggcgtctttc ttagcagctt
    18961 tggctcgacc gctaacccgc aaatgtttgc ccttgccctt gcccttgccc ttgcccttgc
    19021 ccttgcccgt gaacatgccg acaaaaccgg acgtgaggcg gtgatgttca gctctttttt
    19081 acgcgctcag gtttcactgg catgggtcat tggcccaccg ctggcttatg cgttagcgat
    19141 gggtttcagc tttacggtaa tgtatctgag cgcagcggta gcgtttattg tttgcggcgt
    19201 gatggtgtgg ctgtttttac cgtcgatgca aaaagagctt ccgctggcga ccggcacggt
    19261 tgaagcgccg cgccgtaacc gtcgcgatac gctactgctg tttgtcattt gtacattgat
    19321 gtggggctcg aacagcctgt acatcatcaa catgccgcta tttattatca acgaactgca
    19381 tcttcccgag aaactggccg gtgtgatgat ggggaccgcc gccgggctgg aaatcccgac
    19441 gatgttgatt gccggatatt tcgccaaacg tctgggtaag cgtttcttaa tgcgcgttgc
    19501 tgccgtgggt ggcgtctgtt tttacgcagg aatgctgatg gcgcattcac ctgccattct
    19561 gttgggcttg cagctgctaa atgctatttt tattggcatt ctgggcggca tcgggatgct
    19621 ctattttcag gatttgatgc ccggtcaggc aggttcagcc accacgctct ataccaacac
    19681 gtcgcgcgtg ggctggatca tcgcaggatc agtggcgggc atcgtcgccg agatctggaa
    19741 ttatcacgct gtgttctggt ttgcgatggt gatgattatc gccactctgt tttgcttact
    19801 gcggattaaa gatgtttaag gcgcggtcag catttccaga tcgataagcc aggtcatggc
    19861 ctgctgacgc gagttaccgc acatttcagc gctggcttgt aagcctgcac agactttcgg
    19921 tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa tgcagggcgt
    19981 attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag gggcagtgca
    20041 acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta accaccggat
    20101 tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgtgaata ccacttgccc
    20161 gaaaggcccg tcgcgagtac tttgtcgcga tatttttgac attttcgact acaggaattt
    20221 ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca acggcaaact
    20281 gctgctggct aaggatattg atattcagtc gcccactgcc cgcggtgccg ctacgctgta
    20341 caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt tcaaaggtga
    20401 tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt gacttctcct atgtcgatgg
    20461 caacgaatat gtctttatgg ataaagaaga ctataccccg tataccttca ccaaagatca
    20521 gattgaagaa gagttgctgt ttatgccaga gggcggcatg ccggacatgc aggtgctgac
    20581 ctgggacggt caactgctgg cgcttgagct gccgcagacc gttgatctgg aaatcgttga
    20641 aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg cgacattgag
    20701 cactggtctg gtgattcagg taccagaata cttaagcccg ggcgaaaaaa ttcgtatcca
    20761 tatcgaagaa cgccgttata tggggcgtgc tgactaactt cagccgcatg cagaaaaggg
    20821 atagctcagg ctgtcccttt tttaatttat tataccaatc ttctattttg cgcttcctga
    20881 acaccttacg ccctcaattt tcactcgttg attgatcgcc ctcacacttc atcgcattaa
    20941 caatccagac caatttcaat tgctgtcata taactttaca ctgtcattgt taatttatcg
    21001 ttactaagac gtgactccta tgaagacaat tgcctccgtt acgctcccgc atcatgtaca
    21061 cgctccacgc tacgatcgcc agcagttgca atcacgtatc gttcattttg gctttggcgc
    21121 ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc agggcggcga
    21181 ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc agctccgcgc
    21241 acagaaccat ttatataccg tgctggagaa aggtgcggac ggcaatcagg tgataattgt
    21301 cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa ttattgagaa
    21361 attttgcgag ccacaggtgg caattgtttc cctgacgatt accgaaaaag gctattgtat
    21421 tgacccggcc accggtgcac tcgacaccag taatccgcgg attattcacg atctacaaac
    21481 ccctgaagaa cctcactccg caccgggtat tctcgtcgaa gcactgaaac gccgccgtga
    21541 gcgcggcctt acaccgttta ccgtgctctc ctgcgacaat attcccgaca atggccatgt
    21601 ggtgaaaaac gcggtgctga gaatggcaga aaaacgttcg ccagaactcg ccgggtggat
    21661 aaaagaacac gtcagttttc cgggaaccat ggtcgaccgc attgttccgg ctgcaaccga
    21721 cgaatcactg gcagaaatca gccagcatct gggggtaaat gatccctgcg cgattagctg
    21781 cgaaccgttt atccagtggg tggttgaaga taacttcatc gctgggcgtc ctgcctagga
    21841 agtcgcaggt gtacaaatgg tgaatgatgt cctgccatgg gaagagatga aactgcgtat
    21901 gcttaatggc agccactctt ttctcgctta tctgggttac ctctcaggat tcgcccatat
    21961 cagtgattgc atgcaggatc gcgtatttcg ctatgctgcc agaacattaa tgctggatga
    22021 gcaagcgccg acactgcaaa ttaaagatgt cgatttaaca caatatgcgg ataagttaat
    22081 tgcacgtttt gctaatccgt cgctgaaaca taagacctgg caaatcgcga tggatggcag
    22141 ccagaaatta ccgcaacgta tgctggcagg tattcgcata catctggggc gtgaaacgga
    22201 ctggtcgttg ctggcattag gcgttgctgg ctggatgcgt tacatcagcg gcgttgatga
    22261 tgccggaaat gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg aacttgttgc
    22321 gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag ttttcggtga
    22381 tgatctgcca gataacccgc attttgtgca ggccatcgaa caagcctggc aacaaatcgt
    22441 acaattcggc gcacatcagg cgctattaaa caccctcaaa atttaacgat ttctgcggtt
    22501 aaagcggatg aagctcacct tcgtccgctc tccccttctc ttttctgcct tttttagcca
    22561 agattaacgc tcagttaact taccagagta ttgcggagcc gttgtgacca ggaccaacct
    22621 catcaccggt tttctcggca gcgggaaaac cacgtcgatt cttcatctgt tagcccataa
    22681 agatcccaac gaaaaatggg cggtactggt taatgaattt ggggaagtcg gaattgatgg
    22741 tgctttgctc gccgatagcg gcgcattgct gaaagagatc cccggcggct gcatgtgctg
    22801 cgttaatggt ttacccatgc aggtagggtt gaatacctta ctgcgtcagg gaaaaccaga
    22861 ccgcttgttg atagagccga ccgggctggg ccatccgaaa cagatcctcg atctgttaac
    22921 cgcaccagtc tatgaaccgt ggatagatct gcgcgccacc ttgtgcattc tcgatccacg
    22981 cctgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc tggctgccgc
    23041 agacatcatt gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc aagcgctaca
    23101 gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt cacagtgagc atgggaaagt
    23161 tgacggtcat cttctggatt tgccgcgtcg caatttagcc gagttgcccg ccagcgccgc
    23221 gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc cagagcatca
    23281 acgctggcgt cgcagtctga acagcgggca aggatatcag gcctgcggct ggatattcga
    23341 cgctgataca gtattcgaca ccattggcat tctggaatgg gcgcgacttg caccggtgga
    23401 acgcgtcaaa ggcgtgctgc gtattcccga agggctggta cgaatcaacc gtcagggcga
    23461 tgacctgcac attgaaacgc aaaacgttgc gccaccggac agccgtattg agctgatttc
    23521 cagcagcgaa gctgactgga atgccttgca gagcgcgctg ttgaagcttc gtttagcgac
    23581 taccgcgtaa ggttgcctgc gtttttcagt aagataatta gagaaaatat gattaaaaat
    23641 ttgccgcaaa tagtgttgtt gaatattgtc ggccccgcgc tgtttctttc ctggtatatt
    23701 cccgttaatc atggattctg gttgccgatt gatgcggata ttttttattt ctttaatcag
    23761 aaactggtcg aaagtaaggc ctttttatgg ctggttgcat tgaccaacaa tcgcgccttc
    23821 gacggttgtt cactgctggc gatgggtatg ttgatgctga gtttctggct gaaagaaaac
    23881 gcccctggca gacgacgtat cgtgattatg ggtctggtca tgctattaac tgcagtggta
    23941 ttaaaccagc tgggtcaggc attaattcct gtaaaacggg ccagcccaac attgactttt
    24001 accgatatta accgcgtcag cgaactgctc tctgtcccca cgaaagatgc ctcacgagat
    24061 agctttcccg gcgatcacgg catgatgctg cttatttttt cggcattcat gtggcgttat
    24121 ttcggcaaag ttgcaggcct tatcgccctt actatttttg tggtttttgc atttcccaga
    24181 gtaatgattg gcgcacactg gtttactgac atcattgtcg gttcgatgac cgtgatattg
    24241 atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc gattaatcac cttttttgac
    24301 aaatcactac caggaaaaaa caaacatttc caaaacaaat aactcacagt aattaacatc
    24361 atcagggtta tttttatagt gaggataatc ctgatgatgc gcaccgtgct ttcatctatc
    24421 gaacgcaaaa atcattctct aagtaaatga atggattgca tgcgtttcac tcaattgtac
    24481 tttaattgac caaccccgct tattaacttt ctgtatcact ttttcttata aaaaatcatg
    24541 taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg aactcgtttt gcctcggcgg
    24601 tagattatcc tcacagcata taattttgtg cgttagtcca caaatttggc cttaaggaat
    24661 tgtttcaaca tgcccaggta attagcctcg tgtcgcttgg cattttttta taacgatatt
    24721 tgtcgttaag gacttcaagg gaaaacaaac aacatggtca aatctcaacc gattttgaga
    24781 tatatcttgc gcgggattcc cgcgattgca gtagcggttc tgctttctgc atgtagtgca
    24841 aataacaccg caaagaatat gcatcctgag acacgtgcag tgggtagtga aacatcatca
    24901 ctgcaagctt ctcaggatga atttgaaaac ctggttcgta atgtcgacgt aaaatcgcga
    24961 attatggatc agtatgctga ctggaaaggc gtacgttatc gtctgggcgg cagcactaaa
    25021 aaaggtatcg attgttctgg tttcgtacag cgtacattcc gtgagcaatt tggcttagaa
    25081 cttccgcgtt cgacttacga acagcaggaa atgggtaaat ctgtctcccg cagtaatttg
    25141 cgtacgggtg atttagttct gttccgtgcc ggttcaacgg gacgccatgt cggtatttat
    25201 atcggcaaca atcagtttgt ccatgcttcc accagcagtg gtgttattat ttccagcatg
    25261 aatgaaccgt actggaagaa gcgttacaac gaagcacgcc gggttctcag ccgcagctaa
    25321 taaaccgttt ggatgcaatc ccttggctat cctgacgagt taactgaaag cactgcttag
    25381 gcagtgcttt tttgttttca ttcatcagag aaaatgatgt ttccgcgtct tgatccaggc
    25441 tatagtccgg tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat taacggaatc
    25501 atgttcatac gcgctcccaa ttctggacgt aagctcctgc ttacctgcat tgtagcaggc
    25561 gtgatgattg cgatactggt tagctgcctt cagtttttag tggcctggca taagcacgaa
    25621 gtcaaatacg acacactgat tatcgacgta caaaagtatc tcgataccta ttttgccgac
    25681 ctgaaatcca ctactgaccg gcttcagccg ctgaccttag atacctgcca gcaggctaac
    25741 cccgaactga ccgctcgcgc ggcgtttagc atgaatgtcc gtacgtttgt gctggtgaaa
    25801 gataaaaaaa cattctgttc atctgcgact ggtgagatgg acattccact caatgaattg
    25861 attccggcgc tcgacattaa taaaaacgtc gatatggcga tcttacccgg cacgccgatg
    25921 gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa aaatagcggt
    25981 gtgtttgccg ctctgaatct caacctgacg ccttctctct tttatagttc acggcaggaa
    26041 gattacgatg gcgtcgccct cattattggc aatactgcgc tatctacctt ttcttcacgt
    26101 ttgatgaacg ttaatgaatt aaccgacatg ccggtccgtg aaactaaaat tgcgggcatt
    26161 cctctgaccg ttcggcttta tgcggatgac tggacatgga acgatgtgtg gtacgcattt
    26221 ttactgggcg gcatgagtgg aactgtcgtt ggcctgcttt gctattacct gatgagcgta
    26281 cgtatgcgcc ccggcagaga aatcatgacc gccatcaagc gcgaacaatt ttacgtggcg
    26341 tatcaaccgg tggtggatac acaagctttg cgggtaacgg gcctggaagt actgctacgc
    26401 tggcggcatc ctgtagcggg agaaattccc ccggatgcct tcattaactt tgccgaatcg
    26461 caaaagatga ttgtgccgct gactcagcac ctgtttgagt taattgcccg cgatgccgca
    26521 gaattagaaa aagtactgcc ggtaggcgtc aaatttggca ttaacattgc gccggaccat
    26581 ctgcacagcg aaagctttaa agcagatatc cagaaactgc tcacttccct gcccgcacac
    26641 catttccaga ttgtgctgga aattaccgag cgcgatatgc tgaaagagca agaagccaca
    26701 caactcttcg cctggctgca ctcggtcggc gtagaaattg ctattgatga cttcggcacc
    26761 gggcacagcg cgcttatcta tcttgagcgt tttacgctcg attatctgaa aattgaccgt
    26821 ggatttatca acgccatcgg tacggaaacg atcacttcac ccgtacttga cgcggtgctg
    26881 acgctggcga aacgtctcaa tatgctgaca gttactgaag gggtcgaaac gccggaacaa
    26941 gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag gctactggat tagtcgcccg
    27001 ttaccgctgg acgattttgt tcgctggctg aagaaaccgt atacgccgca gtggtaaggt
    27061 gtgcttacgt cccttattat tcatagtgaa agcatgccgg attgcggcta atgatgagta
    27121 aaaggaaatc cgttgcagat gattgtgcgc atactgctgc tgtttatcgc tctgttcacc
    27181 ttcggtgcgc aggcgcaggc tatcaaggaa agctatgcct ttgccgtgct gggcgaaccc
    27241 cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc aaaaggtggg
    27301 cagataacgt tgtcagccct cggtaccttc gataatttca accgctatgc actgcgcggc
    27361 aacccgggcg cacgcaccga gcagctgtac gacacgttat ttacgacttc cgatgacgaa
    27421 ccaggcagtt attacccgct gattgctgaa agtgcacgct atgctgacga ttattcctgg
    27481 gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg gttctcccat tactgcccgc
    27541 gatgtagagt ttacttttca aaaatttctg accgaaggcg tgccgcaatt tcgtctggtc
    27601 tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg tgcgcattga gttagctaaa
    27661 cccggcaaag aagatatgtt gagtctgttt tcgctgccgg tatttccaga aaagtactgg
    27721 aaggatcaca aacttagcga cccgctcgcc acgcctccgc ttgccagtgg tccgtaccac
    27781 gttacgtcct ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga ttactgggca
    27841 gcaaacttac cggtaaaccg tggacgctgg aatttcgaca ccattcgcta cgattattac
    27901 ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt gcgtatggaa
    27961 aacgacgcta aaaactgggc cacgcgttat accggtaaaa atttcgataa aaaatacatc
    28021 atcaaagatg agcaaaagaa cgaatcagcc caggatacgc gctggctggc gtttaatatc
    28081 caacgtgcgg tattcagcga tcgccgggtg cgggaagcaa tcactctcgc ctttgacttt
    28141 gaatggatga acaaggcgtt gttttacaac gcctggagtc gcacgaacag ttattttcaa
    28201 aataccgaat acgcggccag aaattacccc gacgccgcgg agctggtgct tctggcacca
    28261 atgaaaaaag atctaccgcc agaagtcttc acacaaatct accagccgcc ggtatctaaa
    28321 ggcgatggct acgatcgtga caacctgtta aaagccgaca aacttctcaa cgaagcgggc
    28381 tgggtgctga agggtcagca acgcgttaat gccacaacgg gtcagccgct cagctttgaa
    28441 ttattgcttc ccgcaagcag caatagtcag tgggtattgc cgttccagca cagcctgcaa
    28501 cggctgggta tcaatatgga cattcgcaag gtggataact cgcaaatcac taaccgcatg
    28561 cgcagtcgcg actatgacat gatgccgcgc ctatggcggg cgatgccgtg gcccagttcc
    28621 gatttacaga tttcctggtc atcggaatat atcaattcca cttataatgc ccccggcgtg
    28681 caaagcccgg ttatcgactc gctgatcaac caaattattg ccgcgcaggg aaataaagaa
    28741 aaattactgc cgttggggcg agcactggat cgcgtattaa cgtggaatta ttacatgctg
    28801 ccaatgtggt acatggcgga agactgtctc gcctggtggg ataaattctc ccaacccgct
    28861 gtacgccctg tttacagcct gggtatcgat acctggtggt atgacgttaa taaagcggcc
    28921 aaactgccgt cagccagaca acagggagag tagatgggcg cttacctgat tcgccgtctg
    28981 ttgctggtga tcccaacgtt atgggctatt atcactatca actttttcat tgtgcaaatt
    29041 gcgcctggcg gtccggtcga tcaggccatc gccgccattg agtttgataa tgccggagta
    29101 ttacccggcg caggcggtga aggtgttcgt gccagccatg cgcaaacggg tgtcggcaat
    29161 atcagcgaca gtaattaccg tggcggacgc ggattagatc cagaagtgat cgctgagatc
    29221 actcatcgct acggtttcga taagccgatc cacgaacgtt acttcaaaat gctctgggac
    29281 tacatccgct ttgattttgg tgatagcctg tttcgcagcg cctcggtgct gacgctgatt
    29341 aaagacagtc tgccggtttc catcaccctc ggattgtgga gcacgctgat tatctatctg
    29401 gtgtcgattc cgttaggcat tcgcaaagct gtttataatg ggagtcgctt tgacgtctgg
    29461 agtagcgcat ttatcatcat cggctacgcc attccggcct ttttgtttgc catcctgctg
    29521 attgtcttct tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg cctggtttcc
    29581 gctaactttg attcgctgcc gtggtatcag aaaatcaccg attatctgtg gcatatcacg
    29641 ctaccggtgc tggcgacagt gattggtggc tttgcggcgc tgaccatgct gaccaaaaac
    29701 tcattccttg atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa aggggtaagt
    29761 gagaaaaaca ttctctggaa acatatgttc cgcaacgcca tgctgctggt gattgccggt
    29821 tttccggcga cgtttatcag catgtttttt accggctcgc tgctgattga ggtgatgttt
    29881 tcactcaatg gtcttggctt actgggctac gaagcgaccg tctcgcgcga ttatcctgta
    29941 atgtttggta ccttgtatat tttcaccctg attggcctgc tgctgaatat tgtcagtgat
    30001 atcagctata cgctggttga tccgcgtata gattttgagg gacgttaatg tcgcgactca
    30061 gccccgtcaa tcaggcccgt tgggcgcgtt ttcgtcataa ccgtcgcggc tactggtcgt
    30121 tatggatttt cctcgttttg tttggtttga gtttgtgttc tgaacttatc gctaacgata
    30181 aaccgttgct ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa aactacagcg
    30241 aaagcgattt tggcggcccg ctggcaagtc aggctgatta tcaggacccg tggctgaaac
    30301 aacggctgga aaataacggc tgggtactgt gggcaccgat tcgctttggt gctaccagta
    30361 tcaactttgc taccgataag cccttccctt ctccaccctc ccggcaaaac tggctgggaa
    30421 cggatgcaaa cggcggcgat gtgctggcac gcattctcta tggcacgcgg atctcggttc
    30481 tgtttggcct gatgctgact ctctgttcca gcgtgatggg cgtgctggcg ggggcgctac
    30541 aaggctatta cggcggtaaa gtcgatctct gggggcaacg ctttattgaa gtatggtcgg
    30601 ggatgccgac gctgtttttg attattttac tttccagcgt cgtacagcct aacttctggt
    30661 ggctgctggc aattactgtc ttgtttggct ggatgagtct ggtcggcgtg gtgcgggcgg
    30721 agtttttacg tactcgtaat ttcgactaca ttcgtgcggc acaggcgctt ggcgtcagcg
    30781 atcgcagtat catcctgcgt catatgttgc ctaatgccat ggtcgcgacc ctcacctttt
    30841 taccgtttat tttatgtagt tcgataacca ccctgacctc gctcgatttc ctcggcttcg
    30901 gtctaccgct cggttcaccg tcactcggtg aactgctgtt acaagggaaa aataaccttc
    30961 aggccccgtg gcttgggatc accgccttct tgtcggtggc gatattattg tctttgctga
    31021 tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg tagcatgacg
    31081 caaactctgt tagcgattga aaatttgtcg gtgggttttc gccatcagca aaccgtacgt
    31141 acagtagtca atgatgtttc actacagatt gaggctggcg aaacgctggc gctggtgggt
    31201 gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct cccttccccg
    31261 ccggttgaat atctctccgg cgatattcgt tttcatggcg aatcgctgct tcatgccagc
    31321 gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca tgatttttca ggaaccgatg
    31381 gtgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct ttcactccac
    31441 cgcgggatgc gtcgggaagc ggctcgtggc gaaattctta actgccttga tcgcgtcggt
    31501 atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg cggcgaacgc
    31561 cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat tgccgatgaa
    31621 ccgaccaccg cactggacgt ctctgtccaa gcgcagattt tacagctgtt gcgcgaactg
    31681 caaggcgagc tgaatatggg catgctgttt attactcata acctcagcat tgtcagaaaa
    31741 ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa taacgccgct
    31801 acgctatttg cctcacccac tcatccttac acacaaaagc tactcaacag tgaaccgtca
    31861 ggcgatccgg taccgttgcc agaacctgcc tcaacgttgc tggatgttga acagcttcag
    31921 gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa tgtggtggtg
    31981 aaaaacatca gttttacgct acgggcgggt gaaacactgg gtttagtggg cgagtctggt
    32041 tccgggaaaa gcacgacggg actggcgctg ctgcgactga ttacctctca gggcagcatc
    32101 gtctttgacg gtcagccact gcaagattta aatcgccgcc agctgttacc tactcgtcat
    32161 cgtattcagg tggtatttca ggatccaaac tcctcgctca acccacgact caacgttttg
    32221 cagattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc acaacgcgaa
    32281 caacaagtga tagccgtgat gcatgaagtg ggattagatc ctgaaacacg ccaccgttat
    32341 ccggcggagt tctctggtgg tcagcgacaa cgtattgcga ttgccagggc attaattctt
    32401 aagccctcgc tgatcatact tgatgaaccg acatcatcac tcgacaaaac ggtacaggcg
    32461 caaatattga cgcttttgaa atcattgcaa caaaagcatc aactggccta tttgtttatc
    32521 agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact gcgacaaggg
    32581 gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgcca caccgcagca ggagtatacg
    32641 cgtcagttac tggcgttgag ctgacgctta aaaaggattg tagtttgaaa agggttcggc
    32701 aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct ggcgatttac
    32761 aaacaggcac ggctcacctt cacactccag caccgaacat ggaacttcga tgtcgcttaa
    32821 cgcctggctt agtttataca tcaccgacca tgcattatca ccatccgggc taagcaattt
    32881 aagcgccacc aggctgttgt caccgcacgg tccattttgc ggaatcggtt caaccttcga
    32941 acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt cgatggacga ttgagagttg
    33001 taatgaagtc acttattttc cccggaagta cattcacttc acaatttgtt tacatcaatt
    33061 ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc ctcgcctgcg
    33121 tggcctctat ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt gcggggctat
    33181 atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg actttttaca
    33241 taaattgatt ttacataaaa taaacatata tcggggaaat gatagatttg tggttgacat
    33301 gcatcaacaa aggaagcctt ttaccttcct cgttgtgcaa tagatcaccg ttttttcggc
    33361 cgactggcgt acagacagaa gagaatggag ctggttgcgc agaatgcaat tgaccaaatc
    33421 atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat tgcccctatg
    33481 ccaaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg aaactcatca
    33541 agaatgaccg ccatcgcatt ggatgacacc atcgacacgc agcccacaaa cgccgcaacg
    33601 ccaaccacca gcgaccagaa tcccagcccc agcagcgcac tgatgaccat ccacgctgcc
    33661 ataataaatt gtatccacaa ccccgagcgg aacatattta acgcgccaat gcggcggacg
    33721 aagcggctgt taaagatggt catcacgaac agaaaaacaa tgtttagcgc aaagtaataa
    33781 ccaaagtttt ccggcgcgac gtggttaatt tcaatataaa caaacggtcc ggcgcttaag
    33841 aatgagaaca tcccggcaaa gctgaaacca ctggcaagca tgtagctcag gacacgttta
    33901 tggcggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaatgg ctgacgacgc
    33961 tccggtggta aggtttcttt aatcaggaag aaaatcattg ccgaagccag aatcgccgct
    34021 aatgccagga tccagaagat gtaatgccag ctcagccaca ccagcaccca gccgccaact
    34081 atcggtgcca tcagcagtgc aatggtcgtc accagcatga caaacgacat catccgcgag
    34141 aactcttctt tcgggtaaat atcacgcatc aaagcgttga taaccacgct ggccgcagcc
    34201 gcagccagcc cgtggaagaa acgcatcaca atcagctgat cgatggtttg tgccaacgca
    34261 cacgccaccg cggcggcggc aaacaccagc gtcccgccaa gaaccaccgg cttacgcccg
    34321 aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag aatataagta
    34381 ctgagggtca tctgcgtact gcccgccgat acgccaaact gcgctgaaat taccggtagc
    34441 gcgggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc aaggataaaa
    34501 acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa gtcattgaaa
    34561 agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc gggggctaaa
    34621 tcagcatcca gcgtaatacc gccaatacgt tcacgatgca gctcaaccac gtggttaccc
    34681 acggcggcga acatgcgttt cacctgatga taacgccctt cgctgatggt cagacgaacc
    34741 tgcgttgggg taatcacttc aagcaccgcc ggcttagtga gatctttttc gttatgcagc
    34801 tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta caggtgattc cagtgtcacc
    34861 agataggtct tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca ctgaccatca
    34921 tcagtcatca gcaccagacc ggtggtatcg atatccagac gtcctgcggc gtgcagtttc
    34981 cacgctaccg gttcatccag aaaatagagc accgtcgggt gatccgggtc gtccgtggag
    35041 caaacatagc cctgaggctt attgagcatg aagtaacgtg gaccgtgttg ctgcgccagc
    35101 gggttgccat cgtaagcgac atcatgttca gggagcagtt tgaacgctgc attacgaacg
    35161 atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat agcacggcta
    35221 acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt gcctgtaaaa
    35281 aacggaggtc gggtaatgac ccgaaaatct gaactgttgt ctgcccagta tagcggtcta
    35341 ataacttccc tcaagggaaa aagaaccgtg gcatactatt agcagaataa tctacctacg
    35401 cgaaaccatg atttttacac tccgcccata tcagcaagaa gccgtggatg ccacgctcaa
    35461 ccattttcgt cgtcataaaa cccctgccgt tatcgtgctg cccaccggcg caggtaaaag
    35521 cctggtgata gcggaactgg cacggctggc gcgtggtcgc gtactggtgc tggcacacgt
    35581 taaagaactg gtggcgcaaa accatgcaaa gtatcaggcg ctggggctgg aagccgatat
    35641 ttttgccgcc gggctaaagc gcaaagagaa ccacggtaaa gtggtatttg gcagcgttca
    35701 gtcggtcgcc cgtaatcttg atgcctttca gggtgaattt tcgctgttga ttgtcgatga
    35761 atgccaccgt attggtgacg atgaagagag ccagtatcag caaatcctca ctcacctgac
    35821 taaagtgaat ccccacttac gcctgctggg gctgactgcc acgccttttc gattgggcaa
    35881 aggctggatc taccagttcc attatcacgg catggtacgc ggcgatgaga aagccctttt
    35941 ccgtgactgc atttatgagc tgccgctgcg ttatatgatt aaacacggct atctgacgcc
    36001 gccagaacga ctggatatgc cagtagtgca atacgatttc agccgcttgc aggcacagag
    36061 taacgggctg ttcagcgaag ctgatctcaa ccgtgagctg aaaaaacagc aacgcattac
    36121 gccacatatc atcagccaga tcatggagtt cgccgaaaag cgcaaagggg taatgatttt
    36181 cgccgccacc gttgaacacg caaaagagat tgtgggatta ctgcctgccg aagatgctgc
    36241 actgattact ggcgacaccc ccggcgctga gcgcgatgtg ttaattgaag attttaaagc
    36301 ccagcgtttt cgctatctgg tcaacgtcgc agtactgacc accggatttg acgccccgca
    36361 cgtcgatctt atcgccattc tgcgccctac cgaatcagtg agtctttacc aacaaattgt
    36421 cgggcgcggt ctgcgtctcg ctccgggcaa gactgattgt ttaattcttg attatgcggg
    36481 taatcctcac gatctctacg cgccggaagt tggtacaccg aaaggcaaaa gtgacaacgt
    36541 tccggtacag gttttctgcc ctgcctgcgg ttttgccaac accttttggg ggaaaacgac
    36601 cgccgacggg acattgattg aacactttgg tcgccgctgt cagggatggt ttgaagatga
    36661 cgacggtcat cgcgaacagt gtgacttccg tttccgtttt aaaaattgcc cgcaatgtaa
    36721 cgcagaaaat gatattgccg ctcgccgctg ccgcgaatgt gacacggtac tggttgatcc
    36781 ggatgatatg ttaaaagcgg cgctacgact gaaagatgcg ctggtattac gctgtagcgg
    36841 catgtctttg cagcatgggc acgacgagaa aggcgaatgg ttgaaaatca cctattacga
    36901 tgaagacggc gcggatgtga gtgagcgttt ccgtctgcaa acgcccgccc agcgtaccgc
    36961 cttcgagcag ctttttatcc gcccgcatac gcgcacaccg ggcatccctc tgcgctggat
    37021 caccgccgcc gatatcctcg cccagcaagc cttactgcga catccggatt ttgtcgtcgc
    37081 ccgcatgaaa ggtcagtact ggcaagtgcg tgaaaaagtg ttcgattacg aaggtcgttt
    37141 tcgtcgggcg cacgaattac gcggttaatg gtagttttga ttgatgtata aggcgattga
    37201 gtatagaatc tcgcccgctt ttgcatacgc aaagcagatc acttacttgt tgctgggtcg
    37261 cctgtagcag gattaattta agagagaaag aaatgtttac tatcaacgca gaagtacgta
    37321 aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc taacaagttc ccggcaatca
    37381 tctacggtgg caaagaagca ccgctggcta tcgagctgga tcacgacaaa gtcatgaaca
    37441 tgcaagctaa agctgaattc tacagcgaag ttctgaccat cgttgttgac ggtaaagaaa
    37501 tcaaagttaa agctcaggac gtacagcgtc acccgtacaa accgaagctg cagcacatcg
    37561 acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac cccgctccgg cggggttttt
    37621 tgtatctgca gattatgcct gattacggta ttgctatttt ttgcaggcca gataaggcgt
    37681 tcacgccgca tctggcattc agaacacaaa accgattaat tgccgccaga cgtccggcgc
    37741 tgcaattggt cgcgcaaatt cggcggcgtg cctttaatgg tcaaggtatc ggttgctgga
    37801 tcccagaaga tgcgttcacc taacagcatc gcatcaaagt taatagtcaa gccaccgccg
    37861 ctaccggcaa acttcgtcaa ctgacgcagt gtgctacggt ctgccgggaa gctctcttcc
    37921 agttcgtagc ctttttcagc cgcaaactcc gtgaagctca cttcgcttac gcctgccagc
    37981 tctttcgaca gcgattccag ttcgatctct tcgcctgcct gcagctgctc attacagtag
    38041 ctataaacct gctggcgcac gttctgtcgc tctgctttat ccagttgtgc ttcggcagtg
    38101 aaatcatcaa ccgcttgcaa cagcccacgg ttttgcgctt tcgcgttcaa tccttcgctg
    38161 gccccgagga aatccataaa gaaatccgcc actttgcgcc ctactcgccc tttcaggaaa
    38221 gtgagatagc gggtggactc tggattggtt tcccattcgg ttaaatcgat acgcgcaaca
    38281 atatccgcat gattgatatc aagataatgg gttgggttga tgtcgagatt ttcgttaacg
    38341 cgcatactgc tcaggttgct cagaactgcc accagcaaat actccaccgc cagataacgg
    38401 tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat atttcgctaa ttcgtcacgc
    38461 aggcgaccgg ttgccgcgcg gctaaatgcc agaaagtcct cttcgccctg acgctgtaag
    38521 cgcagcgtct gcgccagttc actctcttcg ctaaacaagc cgtaagcttt atttttggcg
    38581 ctatagaccc gatgcagttc tgccaccatc tcaacgacgg tttctgtcgg ttccagcaat
    38641 gaatcgcgca acaccagctc aaggttctgc tcatcacgct tgataagctg gtgcagagca
    38701 atctggttga tatccagact catgataaac tctcctttaa gaccgggcgg tattcaacca
    38761 ccgccggtta agcgacgcaa taaaagatgg cggtcatttt acagaaggca gcgtaatgag
    38821 aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc tacggtaata tgttgccctt
    38881 tcatcaacta actgatttcg atttatgcca caaatttccc gctacagtaa tgaacaggtt
    38941 gaacaattgc tcgcagagct gctcaacgta ctggaaaaac ataaggctcc gaccgacctt
    39001 tccctgatgg tgttaggcaa catggtcact aaccttatca acaccagcat tgccccggcc
    39061 caacgccagg caattgccaa ctcttttgcc cgcgccttac agtcctctat caacgaagac
    39121 aaagcgcact aagggaaaca gataacaggt tatggtaact catcgtcagc gctaccgtga
    39181 aaaagtctcc cagatggtca gttgggggca ctggtttgca ctgttcaata ttctgctttc
    39241 gctcgtcatt ggcagccgtt acctgtttat cgccgactgg ccgacaacgc ttgctggtcg
    39301 catttattcc tacgtaagca ttatcggcca tttcagcttc ctggtgttcg ccacctactt
    39361 gctgatcctc ttcccgctga cctttatcgt cggctcccag aggctgatga ggtttttgtc
    39421 cgtcattctg gcgacggcgg gaatgacgct attactgatc gatagcgaag tctttactcg
    39481 tttccatctc catcttaatc ccatcgtctg gcagttggtt atcaacccag acgaaaatga
    39541 gatggcgcgc gactggcagc tgatgttcat cagcgtgccg gttattttat tgcttgaact
    39601 ggtgtttgcg acgtggagct ggcaaaagct gcgcagcctg acgcgtcgtc gacgcttcgc
    39661 gcgcccgctg gccgcattct tatttatcgc ctttatcgcc tcgcatgtgg tgtatatctg
    39721 ggccgatgcc aacttctatc gcccgatcac catgcagcgc gctaacctgc cgctttcgta
    39781 cccgatgacg gcgcgacgtt tccttgagaa gcatggcctg cttgatgcgc aggagtatca
    39841 acgccgtctt attgagcaag gtaatccaga cgccgtttcc gtacagtatc cgttaagcga
    39901 actgcgctat cgcgatatgg gcaccgggca gaatgtgctg ttgattactg tcgatggcct
    39961 gaactactca cgcttcgaga agcagatgcc tgcgctggca ggttttgctg agcaaaatat
    40021 ttcgttcacg cgccatatga gctccggcaa cactacagac aacggcatct ttggcctgtt
    40081 ctatggcatc tcgccgagct atatggacgg cattctgtcg acccgtacgc ctgcggcatt
    40141 aattactgcg cttaatcagc aaggctatca gctggggtta ttctcatcag atggctttac
    40201 cagcccgctg tatcgccagg cattgttgtc agatttctcg atgccgagcg tgcgcaccca
    40261 atctgacgat cagaccgcca cgcagtggat caactggctg ggccgctacg cacaagaaga
    40321 taaccgctgg ttctcgtggg tttctttcaa tggcactaac attgacgaca gcaatcagca
    40381 ggcatttgca cggaaatata gccgggcggc aggcaatgtc gatgaccaga tcaaccgcgt
    40441 gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg gtggtgatta tcactgccgg
    40501 tcggggtatt ccactgagcg aagaggaaga aacctttgac tggtcccacg gtcatctgca
    40561 ggtgccatta gtgattcact ggccaggcac gccggcgcag cgtattaatg ctctgactga
    40621 tcataccgat ctgatgacga cgctgatgca acgcctgctg catgtcagca cacctgccag
    40681 cgaatattca caaggtcagg atttgttcaa ccctcaacgc cgtcattact gggttactgc
    40741 tgcggataac gatacgctgg caattaccac cccgaaaaag acgctggtgc tgaacaataa
    40801 cggtaaatac cgcacttaca acttacgtgg tgaaagagtg aaagatgaaa aaccacagtt
    40861 aagtttgtta ttgcaagtgc tgacagacga gaagcgtttt atcgctaact gattaattat
    40921 aaatcagtta gcgaaatatc ttacttgcaa tcggtgtgga aaacggtagt attagcagcc
    40981 acgagtcggc acgtagcgca gcctggtagc gcaccgtcat ggggtgtcgg gggtcggagg
    41041 ttcaaatcct ctcgtgccga ccaaaaatcc caagaaaaaa ccaaccctta cggttggttt
    41101 ttttatatct gcaattaatt cgataaacag accgtgacac atcacagcct gtttattttc
    41161 tgttatcaga acgtccagac cacacccgcc tgagtattcc acggcgattc tacccctgcg
    41221 ccattgccat agctgactga caaatgaccg cttaacgtcg gggtaaacga tgaccttatc
    41281 ccagcctgat aaacgccact ggttccagcc acatcattat taaatttgcc atcgtcatta
    41341 actttcacct ggttagaatc ggcgtattcc tgacgcacgg cggctttcag ccagggttcc
    41401 agcgtcgtac cgttttgcag gtccatgtga tagcttaccg ccgttcccgc ttcagcgcgt
    41461 aatatccggg tatttcccac atccgcgcgc atgccgtttg ataacgtgta gtcctgacca
    41521 tctgtggtaa agccggtaaa ggccagatag ggtctaacac tccacaatcc gtcaacccaa
    41581 cggaacccgc tctcaacatg agcacccgcg ccgttactat tgtaatcgcc aaacgctgtt
    41641 gccccattac tcatattgcc atggatggtg ttggcaaaac ggtcaacttt caccacccca
    41701 tcaacatagg caccgttctg atgctcccaa ccggcataag cccccagggt atagctatcg
    41761 atattacctt ttccgccgcg atcaaaacca atatcagaat gagagtaacc aaagaacaag
    41821 ccgcgaattg tactgctttc ttcacgggag aaacggctat cgataccgag cgtcaggccc
    41881 gtcaatgttt gctcaaaacc agctcccgca tcagtggtca cgttgttgcg ggtgttaatt
    41941 gccgaactcc acatcaccgt atcgtaacta acgcctttta cgctaccaag acgctcacgc
    42001 acggtgtcca gttctgcatc aaataccagc ggttgtgcgg ctgccatgtt cagcacatca
    42061 gtggttgaag gggtaatttg cgcgcgattc tctgcgaggc tccagctatg gttgccatta
    42121 tccagcaagg tatattcata cgtaccgata tcaacaacgc ctccggcatt gcccaacgta
    42181 aatgcagcat caccgccgcc cgttgttacc agtgtaaggc tatctcctgc tgccgggctg
    42241 gcaccggtgt ccgtcacgaa tattttgaaa tcacctgttg cctgaccggt gacgttgagc
    42301 tgatcgctct gatgattggc catatcggta cgcatataaa aattgccgtt tcccgacagg
    42361 gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcacagatga acgagtcact
    42421 ggctcagcca tcataatcac atttgcgccg gacatcgcaa ggttggctaa gttgaggcga
    42481 gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt ttcgagcgaa
    42541 acgtccgctt tgctggtatc cacggcacca tgcgttctaa aagaagcgcc ctcagaaacc
    42601 actaccttac ccaccaacat agcgggtgca taatttattt gcggcttcat gacctcaaga
    42661 atgccatcat tccctctgac tgacacgtta accattgtgc cagcccagac attagcgcgg
    42721 ccattgttga ttaccatatt ttcagagatg gcttttgagg aatacgtgat atttccattc
    42781 tggtctgatc gaccgagatc atataccgca tttgcctgca ctttcgtacc agtatccttt
    42841 cccagcgatt gcatagtggc atgctcatca aggatagtgt caatggcctg cgtgtcctcc
    42901 ataacaatga gcccggaacc atcatccagt tcataatttt ttgacacacc atcctttata
    42961 ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga gacaattaat
    43021 ttcccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac ctccagtaag
    43081 ccgccactat ccacagtagt attataagcg aagtcatttt cttcaactcg caaacttccg
    43141 ccgttttcca acaacatatt attagcaata ccattcttga tatcgaactg accgagacgg
    43201 tttgttccga atacctccat ggcccgcgtg gttgttttaa tagcaccgcc tgctttctga
    43261 tctaccgcaa gcgcaaatcc cccctcttta acctccagta gtcctccctt ttcgatagcg
    43321 gcaaaaacgg tccccccatc tttttcaacg attaagcgcc cgcctttaat cgtggtatca
    43381 ttcgctgggc cataaacctg tagcacacca ttattgaggg tagtctggtt tgccgcgcca
    43441 cccatttgaa caatttgata accatcattt aatactgagt attctgcggt accattcatt
    43501 tcaatgtatt gataccctcc gttaatttca gtattactac ttacaccaaa ttcttttata
    43561 tgctgttctc cgccaggatt gattttcgta ttattggcgg tcccataaac cagctggata
    43621 tctttttcca ggataacacc atcaactgtc tcatcatact caactgttga tgccaatgct
    43681 atgggtgaat ataacgctgt agccaccaga acagatattg cggaacgaca aagagaaaca
    43741 gaaccagatt gatgcattga gctttcatcc tatgaaatta attgcagtta aaagcattgg
    43801 gtacagaaaa tacccatagc tccatacccg gagtcagttt ttaaaaactg tttaaagaaa
    43861 tgcacaagta ttgtgattga ttttttagtt gtttttcttg atgagaagct gatgcaaaat
    43921 tccgtcttta taatgaaaat gatgccaaag cgaacgacaa ggttgtagtt ttcactacat
    43981 gtccatacat aaaatggggt aacattcacg cgcctggtag cgttaccaac gctacgctca
    44041 aacataatga ttctaataaa acctcaggag actactatgc ctgaagcaac gccttttcag
    44101 gtgatgattg tggatgatca tccacttatg cgacgcggtg ttcgtcagtt actggagctt
    44161 gatcctggct ttgaagtggt cgccgaagcg ggcgacggcg cgagcgcgat cgatctggcg
    44221 aatagactgg atatcgacgt gatcttgctg gatctcaata tgaaaggtat gagtggcctg
    44281 gataccctca atgccttgcg cagggatggc gttaccgcgc aaattattat cctgaccgta
    44341 tccgatgcct ccagcgatgt ctttgcgctg atagacgcag gcgcagacgg ttatctgttg
    44401 aaagacagcg acccggaagt attgctggaa gcgattcgta ccggagcgaa aggcagcaaa
    44461 gtctttagcg aacgcgtcaa tcagtactta cgtgaacgtg aaatgtttgg cgcggaagaa
    44521 gatcccttca gcgtgctgac ggagcgcgag ctggatattc tgcacgagct ggcacagggg
    44581 ctgtcaaata aacagattgc atcggtgttg aatatttccg agcagacagt aaaagtacat
    44641 attcgcaatc tgctgcgtaa actcaatgtc cgctcacgcg tggcggccac cattctgttc
    44701 ctgcaacaac gcggggcaca ataaaaatag cccgatggat ttatcatcgg gctgagattt
    44761 atgacaaacg caaaactgcc tgatgcgcta cgcttatcag gcctacgtgg atcgatcaat
    44821 ttattgaatt tacacaattt tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa
    44881 ctaagcgcac tttgtcatca gtctagcccg atggcatcac catcgggcct ctttttattt
    44941 actctcctgc ggcgacaaat gttgcatcgc ctgcgcgata ctacgttcaa tcaccgcacg
    45001 gcgagtatcg ttggcaggta agagtttcaa catcatctcc cacgcggcaa cggcttcgcc
    45061 aaatcgctgc tgctcaaagg cattaaacgc atacatgctt agcacacgga tattgctatg
    45121 gtccgttctc accagctgac gtagcagttc accgccgagg cggttgtcgt tgggatcaga
    45181 tgaacgagtc aacgcttcag cgtatcccag tgcggcatcg ctgttcttcg gatcgaggcg
    45241 atacgcagta gcgtatgcat cggtggcgat actggcgtta cccagcgcca tgccaacgcg
    45301 gcccaacata atccagcctt ctatatctcc cggatttttt tgcagttgag tacgcatccc
    45361 cagcgcaagg cgcgacatct cttcttcgtt gagcggatcg gctttcgggt ccagcgccct
    45421 gtccagcaac gccggagcct gtgccgtggc ctgctgccag attttcacct gctgataatt
    45481 gccagtctgg tagtagctga cgccagccac aattaacgcc accacaatac ccggcagata
    45541 aacaacatat ccggcacgct taccttccgg cacgctttgt tcaggaaacg cttccggcac
    45601 cacgcgtacc cgacgccgcg agcgggcgta tatgacccaa ccgccaatgc caatagccac
    45661 tactggcagc acccacagca gcacggtcag cggcgttaac ggcggatcgt aagtgacgaa
    45721 gttgccgtaa cgcgccacca tataatcgac aatctctttt ttacttttac cttcctgcat
    45781 cagttcatac actttctgac gcaggtcggt ggcaatcatc gagttggaat cggcaatgct
    45841 gttgttctgg catttcgggc agcgcagttc ttcagtgagc tgacggaact gttgttcctg
    45901 tgcttcatct ttaaactgca acacgtcgat ggtcgccagc gctgagccga agatcatcag
    45961 catcagcacg cccaataaaa acctcattgt gcggcctcct tactgtattt ctcccacagc
    46021 ggcttgatct cttcttccca gacgcgagga ttgagatcgc ccgcatggcg atagcgaatg
    46081 ataccgttgc cgtcaataag gaaggtttct ggcgcgccat agacaccgag atccagccct
    46141 aacatgccgt cgccatcaaa caggcttagc gcgtaaggat tgcccagctc tttcagccag
    46201 ctgattgcct tctggcgatc gtctttatag ttcatgccga ccacgcgaat gccctgcgct
    46261 gaaagctgat tcagatattg atgttccgca cggcaggtcg gacaccaagt cgcccagacg
    46321 ttaagcagta ctggtttgcc ctgagtcagc acatccgcct gataaaactg ccccggattg
    46381 tccagtgatt cgagacgaaa cttcggcaca ggcttgccaa tgagcgccga ttccagattg
    46441 gtcggatcat ccccttcggc attacgcgcc agctgccaca gcagcgccgc ggcaatcgcc
    46501 aggaagataa tcaacggaat taacaatact ttgcgcttca tacggcctcc ggcgcagttt
    46561 tttgcggact cacgcgctta cgatagcgag gatcaaacag acacagcagt ccgcccaacg
    46621 ccatcatcag cccgcccgcc caaatccagc gaacaaatgg tttgtagtaa agacgcacgg
    46681 cccacgcgcc gttttccagc tcttcaccga gcgcggcgta caggtcacgc gtgatgccgc
    46741 cgtcaattgc cgcttcggtc atcatcgacc cggcagtgtt gtaataacgt ttttccgcat
    46801 acagcaccgt ttccggttta ccctctcgcg ttacgccaat agtcgccaca ccgccgcgcc
    46861 agttcgggcc agtcacctct ttgacatcac ggaaggtgaa gcgatattca tgaatatcga
    46921 cgctatcgcc ggacttcatg cgcacatcac gctcaacgct ataattctgg ctaaaggcaa
    46981 tgccaacaat tgtcactgcc agccccaggt gagccgccac catcccccaa taactgaagg
    47041 tggttttcgt gccgcgtgaa atacgcagcg cagcttccgc agttgccagc accgcaatcc
    47101 agcaggccat tgccaggccg agcaccgtca tcgccacaac tttgctttcg aacagccacg
    47161 gcaacagcag agacagcacc agcgtagaga tgaaggcgat aatcaataaa ttgcggattt
    47221 tacgtgggcg atcgcgcccc cagcgcacca gaggaccgac accaagcagt agcgcaaacg
    47281 gcaccatcag ccaggtaaac atggtgttga agaacggttc gccaatcgaa atactgccca
    47341 gtccaagttg cttgtgcacc agcggcagca gcgtccccag caacaccacc agcatcgcgg
    47401 cgaccagcaa aacgttgttc gctaacagca aagattcccg cgaccacagc gcattgttta
    47461 cgcgtgagcg aactttgtgt ccacgcgcgg caaacagcag cagcgaaccg ccaatcacca
    47521 gcaccataaa ggcgaggata aacataccgc gcgccggatc agacgcaaac gcgtgtaccg
    47581 ataccagcac gccggaacgc acgaggaaag tccccaacag acacaacgag aaggcactga
    47641 ttgccagcag taatgtccac gctttgaagc tggcgcgttg ttcagtgacc gccagtgagt
    47701 gcatcagtgc agtccccacc agccacggca taaacgaggc gttttctacc ggatcccaga
    47761 accaccagcc accccagccg agttcgtaat aggcccatgc ggaaccgagc acaatgccga
    47821 gcgtcaggaa aatccacgcc gccagcgtcc acgggcgagt aaaacgcgca taagtgctgt
    47881 ccagacgccc gctcagcaaa gaagcaatgg caaaagcaaa cgccaccgag aaacccacgt
    47941 accccatata aagcagaggc ggatggaaga tcagccccgg atcctgtaac agcgggttaa
    48001 gatcgcgccc ttcaatcgtg aagttcggca gcgtgcgtga gaacgggtta gaggtaaaga
    48061 gaatgaacag caaaaagccg acgctgacca tccccattat cgccagtaca cgggccacaa
    48121 tatccagcgg aatacgctga ctaaaaatcg ccaccgcaaa ggtccagccg ctcatcagca
    48181 gcacccacag cagaagcgaa ccttcatgcg cgccccaggt agccgccacg cgataccaca
    48241 ccggaagctg ggtattggag ttgctggcaa cataggtgac ggtaaagtcg ttgaccacga
    48301 aagcattgac cagcaccaga aatgcgccag ccacagacat aaacagcagc caggcaaaca
    48361 agcgggaaga cgccatcatg cgcgcatctc cgcgcgccac gccccatagc ggatacacgg
    48421 acagcagcag cgcaatccct aacgccaagc acagcagccc gtttccaatt tccggcatca
    48481 tgatgctggg tccttataaa cactcgccgg gcgacggtgg ttagcttcca tcgctttctc
    48541 aacttctggc ggcgtgtagt tttcgtcgtg tttcgccagc acttctttcg cgaggatatg
    48601 attgcctttt tccagctcgc cctgcaccac aacgccctgc ccttcacgga acagatccgg
    48661 caaaatgcct tcgtaagaga catccactga gccttcagca tcgtaaatgg tgaaggtcac
    48721 tttcagcgaa ttgggatcgc gctgcacgct acccggcatc accatcccgc caacgcgcag
    48781 acgctgaccg acttccggca tctgctgagt ttcacgcttg ccgtagagaa tttcccccgg
    48841 cgtataaaag agatcgatat tcgagcgcag cgcatatagc accagaccga tagtcagcgc
    48901 cagccctgcc aacacggcac aggcaatcca caagcggttt ttacggcgaa tattcatgca
    48961 gcctcctgct gttgcgcagc acgtaaacgc gcctcacgcg cccgctgttg cgccacacca
    49021 cgcagaattg cgcgatgttg catcaccgag tgcacgacca aaaccaccag cggaataacg
    49081 gtcatcacca ccgccagcca gacaaaaaag gcgtaaccgc ccattgcgaa aaattcattc
    49141 caggaagcaa atgcaggggt catttacggc ctcttttcag tatcagttca ctcacccacg
    49201 gacggcgttt ttccatcagc aaaatcaaat tacgcatccg catcagcgtc agcgtggcag
    49261 acaggagcag gaagccaaaa atcgaccagc gcagcggcga acgcatcgcc ggatcgatac
    49321 tttgctgcat ccgcgttgat ccctgatgca gggtgttcca ccactccacg gagtaatgaa
    49381 taatcggcag attcaccacg ccaatcagca ccaggatacc tgccgcacgg cccgccagac
    49441 ggcggtcgtc gaaggcgtgc cacagggcaa tcacacccac atacaaaaac agcagcacca
    49501 gttcagaagt cagacgtgca tcccataccc accaggtgcc ccacatcggt tttccccatg
    49561 cagagccggt aaccagggca ataaaggtaa acacggcacc aatgggggcc atcgccgcca
    49621 ccgccaggtt ggccattttc atctgccaga caaggccaat aaacgccgcc accgccattg
    49681 atgcataaat gcccatcgac cagatcgccg caggcacatg caggtagata atgcggtagc
    49741 tatttccctg ctgataatca gccggagcca agccgaatcc ccagatccag ccgacggtaa
    49801 gcacgaccac actggcaatt gccagccacg gtataaacca gccacagatt tgatacagcc
    49861 gtggtgggat cgccagttga tgcagtgttt tccacatagt ttcgatacca gactcgaaca
    49921 aaaatcagta atccagcgtt attgaatgct gattcgtaac gctgccgccg tcgcaaaagg
    49981 acttaatgtc gcggtgcctg ccagcaacgc gcctaaaatt gccagatacc catcaacggg
    50041 caaatgcata gaagccgcgt ccatcgcggc ggtggcaaag atgagtaatg ggatagtcag
    50101 cggtaacacc agtatgctga gcagcacacc accgcgctta agtcccactg tcagcgccac
    50161 gcccggtgca ccgagaaagc caagcgtagg cgttcccagc agcagcgtca gcgccatcac
    50221 ttgccagcca taaacatcca ttcccagtag cattgctacc agtggcgaaa ggatgagtaa
    50281 cggcagaccg gttaccatcc agtgcgccat caccttcgcc agcacaacgg cgggcaaggg
    50341 taacggcaac aacatcaatt gttcaagact gccgtcctgc aaatcgtcac ggaacagtcg
    50401 ttccagcgcc agcaaggatg aaagcagcgc agcaacccag ataatgcccg gtgcaatacg
    50461 cgccagcagt tgcggctccg gaccgatact gagcggaaaa agggtaatta caatcaggaa
    50521 gaaccacagc gggttggcga tttcggcgct atggcgaaac gctacgcgca gttcaagacg
    50581 gaaaatacgc cagaacatca tgcggccctc gtttgcgtca gtgaaatgcg gcgaatttta
    50641 ctttcagcaa cgttgagcgg ctggtgggta gtcagaatca caatcccccc ctgctccgta
    50701 tgctgcgcca tacgctgggt cagacgatcg acgccgttaa cgtcaatcgc ggtaaaaggc
    50761 tcgtcgagga tccataatgt ggcacgggtc aaccacagac gcgctaaagc gacgcggcgt
    50821 tgttgcccgg cagaaagttg atttaccgga atatcttcga atccggcaag cccggcctgc
    50881 gccagggctt ccagacattg tgcggcatcg ccatcgcgat gataaaagtg cagattttct
    50941 aacgccgtca gccgggtttt gatccccggc tgatggccta tccataacag gttttgatgg
    51001 tagctgtcgc gtacctgatg caagggctgc ccttgccaga gaacctcgcc agcgtcaggg
    51061 cgagacaacc ccgtcagcaa acggagaagc gttgtcttcc ccgcgccgtt gctaccggtg
    51121 atttgtaccc actctcctgc gttcagcgta aatgacaagc cactaaataa ggttcgttca
    51181 tcccgctcac aaagtaactc tctggcttca agcataccca cgcaataacc ctgttaaaaa
    51241 cctggctcga cttcacgcat atcgggcagc ttgtgcgcga tccctttatg gcaatcaata
    51301 caggtttgcc catctttcac cgcctggtca tgcatcttcg cggcaaccga tttctgggcg
    51361 gttgtatcca tatactcgaa gttgtgacag ttacggcact cctgcgagtt attgtccttc
    51421 atgcgccgcc actcattctg tgccatcgtc agacgatgag cttcaaattt ctgcggcgtg
    51481 tcaataacgc caaaaatttt accatacagc tctttacttg ctttgagctt gcgtatcatc
    51541 ttcggcacaa actcgtgcgg aacgtgacaa tccggacagg tcgcacggac gccgctacgg
    51601 ttgttgtagt gcacggaatc catgtattcc tgatacaccg tgttgcgcat ttcgtggcag
    51661 ctaatgcaga actcttcggt attggctttt tccatcccgg tgttaaagcc cccccagaag
    51721 acgatgccgc caacaaaacc gatcaacagc agcgtcccca gcgccagatg gctgggggta
    51781 cgccaccatt tccacaggcg cttaatcaga ccaggcttac ggtcagaatt tcccataata
    51841 acctcttatt tcccgtaacc ttttgatggg gtaaaggtat tccccacgat tggcgcggta
    51901 tcggcctgcg gtacgtgaca ttgcagacag aaataacgac gcggagccac ttccgcgccc
    51961 actttgccgt cgctgtccat aaagtgagta ggactgatac gcggcgcgcc agtggtgcga
    52021 tagctttcga caccgtggca ctgcaagcag cgattggtat tggtcgttac ctgataacct
    52081 tcaacgctat gcgggatcat tggcggctga ttcacatagt tcagcggcat ccgatcctgc
    52141 tcttttggca tccgaatggc cccttcctgc gtcccggata cttccggcga ctggctaaaa
    52201 tcgacgccat tagccgccca aaccgcgccg cttaccacca gggccagcat cgccgtccat
    52261 tgacacagcg ctttcttcag gtcatggctt ttcatgattt cgctcccgaa ctccatcgtg
    52321 tagttattgt aaatacatcc tcagaacaga catccacgca gcgaccgcac gtcatgcaat
    52381 cgcggctggt gacctgcacc gggctttgtt catccagcac cggggcacgt agcacatgcg
    52441 gttccgggca aacatgaaaa caatccatac agcggttaca tttctgccga tcgctggccg
    52501 caacggtaat cacacctttg ctacccagca cgccatacag cgcgccaacc ggacaaatgt
    52561 gcccgcacca gccgtgttca acgaccagta gatcaaataa aaacagcgcg agaataagca
    52621 gcgcgccgct gccgaagccc attaccaggc tacgccccat cagagaaacg gggttgatcc
    52681 attcccaaat gagcgtgcca gttaaggctg atcccaccag aatgaccacc aacagcacgt
    52741 agcgaatgtg gcgagggatc gtcgcagact gattgaggtc aaagcgcctg cgtaaccagt
    52801 tcgctaagtc ggtaatcggg ttcagcgggc agacccagct gcaaaataat cgctttcccg
    52861 ccagggcgta gagcacggtg ataatcaccg caccggtcaa cgccaccgtg gcaggcagat
    52921 gaccactggc aagactttgc agcgtcatca gcggatcggt cagcggaacg gtgtcgaata
    52981 acaggctact gctgtagttg ccgtgcaaga tccacacacc aaaccatgga ccgctcaaaa
    53041 acatccccag cacgaagaac tgacaaaggc gacgtaacac cagccaacgg tgactgcgcc
    53101 accagccttt tttctccagc gcctcgcgcc cggcgtcacg tttacgattt gccattgttc
    53161 ccctccagcc agccgaagcg gtaatggtga cctaactccc ctttcgccag tgacagcggt
    53221 aacaccttga ttgccggttg ttccagcacg catacttttt cgcacttacc gcaaccggta
    53281 caggcgtcgc tgtgaaccgt cggcagaaag cgggcgtgct taccggtacg cgtgttgcgc
    53341 tccagctcca aggtgatggc ctcatcaatt ttcgggcatt cgcgataaca aacatcgcag
    53401 cgcagcccct gaaagttgag acagttttcc tggtccacca gtaccgccag ccccatccgc
    53461 gcgtcgtcga tcgattcaat ctcacgatcc agcgcaccgc ttgggcacac tttggcgcac
    53521 ggaatgtcct cacacatttc gcaaggaata tcccgtgcga caaaatatgg cgtgcccgcc
    53581 gacagaccag aggccagcgt cgccagtttt aaggtgtcgt aagggcaagc ctgaacacac
    53641 tgaccgcaac gcacacaggc actggcaaag gcgttctcgt ttatggctcc gggcgggcgc
    53701 aaccgcacgc cagatgcgcg tgcggtttgc tgttgtaacc ccagcgccac acccacggca
    53761 gccagcccgc ctgctgtgcg aacgacatcg cgcagaaagc ggcggcgacc attttgaggt
    53821 ttcgctgacc gggacataat ggcttacacc ttctccagtt tgaccgcgca cttcttgaag
    53881 tccgtctctt tcgagagcgg atcggtcgca tccagcgtca gtttgttaac cagctgtgcg
    53941 gcgtcgaaga acggcatgta caccaggccc tgtggcggac ggttacgacc gcgcgtttca
    54001 acaatcgaga tcacttcgcc acggcgagaa accactttca ctttgtcgcc acggcgcaga
    54061 tcgcgcgctt tcgcatccag cgggtgaata aacaagaccg cttccgggaa ggcgcggtgc
    54121 agttccggca cacggcgagt catactgccg gtgtgccagt gctccagaac gcgtccggta
    54181 gagagccaca ggtcgtactc ttcatccggt gcttccgccg ccggttcgaa cggcagtgcg
    54241 aagatcaccg ctttgccatc cggtttaccg tagaacttat agccttcgcc cgctttcacg
    54301 tacgggtcgt taccttcgct gtaacgccac tgtgtttctt taccattcac caccggccag
    54361 cgcagaccgc gcgctttgtg gtagtcatcg aacggtgcca gatcgtgacc gtgaccgcga
    54421 ccaaaccatg cgtactcttc gaacagccct ttttgcagat agaagcccag ctcgcgggat
    54481 tcatcgttca gctgatcttc cgccagttcg gataccggga atttgctcac ttcgggggtg
    54541 gcatacagaa cttcgtacag cgttttgcca cgcagttccg gtttcttcgc cagcagctct
    54601 tccggccata cttcttcagt tttgaagcgg cgggagaact ggactaactg ccagagatcc
    54661 gatttcgctt cgcctggcgc ctgtacctgc tgacgccaga actgagtacg gcgttcggcg
    54721 ttaccgtaag cgccctcttt ctctacccac attgcggtcg gcaggatcaa gtcggcggcc
    54781 agcgcactga ctgtcggata cggatcggag acgatgatga agttgcgcgg atcgcgccag
    54841 cccggcatac gctcttcatt gatgttcggc ccggcctgca tgttgttggt acacatggtc
    54901 cagtaaacat tcagcttgcc gtctttcagc gcacggtctt gtgccaccgc atgaagaccg
    54961 attttcgccg gaatggtgcc gctcgggata ttccacttct tctcacagat atcacgatgt
    55021 ttctcgttag ttaccaccat gtccgcaggc agacggtgag caaaggtgcc cacttcacgc
    55081 gcagtaccac acgcagaagg ttgcccggtc agggagaacg gaccgcaacc cggctgggaa
    55141 attttgccgg tcagcaggtg caggttgtag accaggttgt tggcccacac accacgggta
    55201 tgctggttga agcccatcgt ccagtaggag atgactttct tgttcggatc ggcatacagc
    55261 tgcgccagtt gttctaactg gtctttcggc acgccggtca tttcagcggt tttttccagc
    55321 gtatactcag caacgaaggc tttgtaatct tcaaagctca tcggttcgga ggcgtcagaa
    55381 cccggattct tcgctgcttt ttccagcgga tgggtcggac gtaaaccgta gccgatgtcc
    55441 gtcgcccctt tgcgcaggtt aacgtgcttg ctgaagaagt cctgatttat cgcattgttt
    55501 tgaatgatat agttggcgat gtagttcagg atcaccaggt cagattgcgg cgtaaagatg
    55561 atgccgttat ccgccagctc gaagctacga tgctggtagg tagaaagcac cgccacggtg
    55621 acgttctggt tagagagacg acggttagta atgcgtgacc agaggatcgg gtgcatctcc
    55681 gccatgttag cgccccacag cacaaacgcg tcagcctgct cgatgtcgtc atagcagccc
    55741 atcggctcat ccataccaaa ggtacgcata aagccaacta ctgccgacgc catacagtga
    55801 cgtgcgttcg ggtcgatgtt gttcgaacgg aagcccgctt tgaacagctt ggacgcggca
    55861 taaccttccc agatagtcca ctgaccagaa ccgaacatac cgatcgattc cggccctttt
    55921 tctttcaggg cggttttgaa tttctcttcc atcacatcga aagcctgatc ccaggtgatt
    55981 ggggtaaatt cgccttcttt gtcatattta ccgtttttca tacgcagcag cggctgcgtc
    56041 aaacggtctt taccgtacat gatcttgggc aggaaatagc ccttaatgca gttcaggcca
    56101 cggttaaccg gtgcgtccgg gtcgccctga caggccacca cacgcccctg ctgcgttccg
    56161 accagaacgc cgcaaccagt accgcagaaa cggcacggcg ctttatccca tttaatggct
    56221 tcctgctgac caacaacggc gcgggcaacg cc
//
LOCUS       DADLSA010000009        56005 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.009, whole genome shotgun sequence.
ACCESSION   DADLSA010000009 DADLSA010000000
VERSION     DADLSA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.009"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..350
                     /gene="cmtB"
                     /locus_tag="JN691_000570"
     CDS             <1..350
                     /gene="cmtB"
                     /locus_tag="JN691_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HAY7242093.1"
                     /translation="LDKNYISENYIQAIKDSTINNGPYYILAPGVAMPHARPECGALK
                     TGMSLTLLEQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSELLCEEEILEQLLTA
                     SSEKQLADIISRG"
     gene            378..1766
                     /locus_tag="JN691_000571"
     CDS             378..1766
                     /locus_tag="JN691_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HAY7242094.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITCLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLPVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1781..3058
                     /locus_tag="JN691_000572"
     CDS             1781..3058
                     /locus_tag="JN691_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7242095.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3055..4020
                     /gene="yggF"
                     /locus_tag="JN691_000573"
     CDS             3055..4020
                     /gene="yggF"
                     /locus_tag="JN691_000573"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY7242096.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQMANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4042..4551
                     /gene="fumE"
                     /locus_tag="JN691_000574"
     CDS             4042..4551
                     /gene="fumE"
                     /locus_tag="JN691_000574"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAY7242097.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4548..5261
                     /locus_tag="JN691_000575"
     CDS             4548..5261
                     /locus_tag="JN691_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAY7242098.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5233..5910)
                     /locus_tag="JN691_000576"
     CDS             complement(5233..5910)
                     /locus_tag="JN691_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY7242099.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(5904..6581)
                     /locus_tag="JN691_000577"
     CDS             complement(5904..6581)
                     /locus_tag="JN691_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY7242100.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6569..6952)
                     /locus_tag="JN691_000578"
     CDS             complement(6569..6952)
                     /locus_tag="JN691_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAY7242101.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(6968..7276)
                     /locus_tag="JN691_000579"
     CDS             complement(6968..7276)
                     /locus_tag="JN691_000579"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242102.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWLHAITLWLLILAIVSTSQL
                     "
     gene            complement(7277..7855)
                     /locus_tag="JN691_000580"
     CDS             complement(7277..7855)
                     /locus_tag="JN691_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY7242103.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(7878..8309)
                     /locus_tag="JN691_000581"
     CDS             complement(7878..8309)
                     /locus_tag="JN691_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7242104.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8681..9700
                     /gene="epd"
                     /locus_tag="JN691_000582"
     CDS             8681..9700
                     /gene="epd"
                     /locus_tag="JN691_000582"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAY7242105.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9750..10913
                     /gene="pgk"
                     /locus_tag="JN691_000583"
     CDS             9750..10913
                     /gene="pgk"
                     /locus_tag="JN691_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAY7242106.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11128..12207
                     /gene="fbaA"
                     /locus_tag="JN691_000584"
     CDS             11128..12207
                     /gene="fbaA"
                     /locus_tag="JN691_000584"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAY7242107.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            12398..13258
                     /locus_tag="JN691_000585"
     CDS             12398..13258
                     /locus_tag="JN691_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAY7242108.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13397..14032
                     /gene="argO"
                     /locus_tag="JN691_000586"
     CDS             13397..14032
                     /gene="argO"
                     /locus_tag="JN691_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAY7242109.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14125..14865
                     /locus_tag="JN691_000587"
     CDS             14125..14865
                     /locus_tag="JN691_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAY7242110.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15032..15928
                     /locus_tag="JN691_000588"
     CDS             15032..15928
                     /locus_tag="JN691_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7242111.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     LTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(15925..17403)
                     /gene="scpC"
                     /locus_tag="JN691_000589"
     CDS             complement(15925..17403)
                     /gene="scpC"
                     /locus_tag="JN691_000589"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HAY7242112.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYNNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17427..18212)
                     /gene="scpB"
                     /locus_tag="JN691_000590"
     CDS             complement(17427..18212)
                     /gene="scpB"
                     /locus_tag="JN691_000590"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HAY7242113.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDNLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18223..19218)
                     /gene="meaB"
                     /locus_tag="JN691_000591"
     CDS             complement(18223..19218)
                     /gene="meaB"
                     /locus_tag="JN691_000591"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HAY7242114.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMMLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDSDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19211..21350)
                     /gene="scpA"
                     /locus_tag="JN691_000592"
                     /pseudo
     CDS             complement(19211..21350)
                     /gene="scpA"
                     /locus_tag="JN691_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(21554..22447)
                     /locus_tag="JN691_000593"
     CDS             complement(21554..22447)
                     /locus_tag="JN691_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAY7242115.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22830..23489
                     /gene="rpiA"
                     /locus_tag="JN691_000594"
     CDS             22830..23489
                     /gene="rpiA"
                     /locus_tag="JN691_000594"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAY7242116.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            23745..24977
                     /locus_tag="JN691_000595"
     CDS             23745..24977
                     /locus_tag="JN691_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAY7242117.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25206..25265
                     /gene="ibsC"
                     /locus_tag="JN691_000596"
     CDS             25206..25265
                     /gene="ibsC"
                     /locus_tag="JN691_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAY7242118.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25598..25657
                     /locus_tag="JN691_000597"
     CDS             25598..25657
                     /locus_tag="JN691_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7242119.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(25758..26360)
                     /locus_tag="JN691_000598"
     CDS             complement(25758..26360)
                     /locus_tag="JN691_000598"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAY7242120.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26381..26564)
                     /gene="ssrS"
                     /locus_tag="JN691_000599"
     ncRNA           complement(26381..26564)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JN691_000599"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26606..26935)
                     /gene="zapA"
                     /locus_tag="JN691_000600"
     CDS             complement(26606..26935)
                     /gene="zapA"
                     /locus_tag="JN691_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAY7242121.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27103..27681
                     /gene="ygfB"
                     /locus_tag="JN691_000601"
     CDS             27103..27681
                     /gene="ygfB"
                     /locus_tag="JN691_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAY7242122.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            27707..29032
                     /gene="pepP"
                     /locus_tag="JN691_000602"
     CDS             27707..29032
                     /gene="pepP"
                     /locus_tag="JN691_000602"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAY7242123.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQDEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29029..30207
                     /gene="ubiH"
                     /locus_tag="JN691_000603"
                     /gene_synonym="visB"
     CDS             29029..30207
                     /gene="ubiH"
                     /locus_tag="JN691_000603"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAY7242124.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLVAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30230..31432
                     /gene="ubiI"
                     /locus_tag="JN691_000604"
                     /gene_synonym="visC"
     CDS             30230..31432
                     /gene="ubiI"
                     /locus_tag="JN691_000604"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAY7242125.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGIQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31506..31589)
                     /locus_tag="JN691_000605"
     CDS             complement(31506..31589)
                     /locus_tag="JN691_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242126.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            31880..32974
                     /gene="gcvT"
                     /locus_tag="JN691_000606"
     CDS             31880..32974
                     /gene="gcvT"
                     /locus_tag="JN691_000606"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAY7242127.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            32998..33387
                     /gene="gcvH"
                     /locus_tag="JN691_000607"
     CDS             32998..33387
                     /gene="gcvH"
                     /locus_tag="JN691_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAY7242128.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33506..36379
                     /gene="gcvP"
                     /locus_tag="JN691_000608"
     CDS             33506..36379
                     /gene="gcvP"
                     /locus_tag="JN691_000608"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAY7242129.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDSHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            36646..37389
                     /locus_tag="JN691_000609"
     CDS             36646..37389
                     /locus_tag="JN691_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7242130.1"
                     /translation="MAIALVTGGSCGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(37446..38879)
                     /locus_tag="JN691_000610"
     CDS             complement(37446..38879)
                     /locus_tag="JN691_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY7242131.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            38924..39235
                     /locus_tag="JN691_000611"
     CDS             38924..39235
                     /locus_tag="JN691_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAY7242132.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            39399..40058
                     /locus_tag="JN691_000612"
     CDS             39399..40058
                     /locus_tag="JN691_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAY7242133.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(40254..41234)
                     /gene="ygfZ"
                     /locus_tag="JN691_000613"
     CDS             complement(40254..41234)
                     /gene="ygfZ"
                     /locus_tag="JN691_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAY7242134.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTALAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            41477..41743
                     /gene="sdhE"
                     /locus_tag="JN691_000614"
     CDS             41477..41743
                     /gene="sdhE"
                     /locus_tag="JN691_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAY7242135.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            41724..42131
                     /locus_tag="JN691_000615"
     CDS             41724..42131
                     /locus_tag="JN691_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242136.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSCMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(42171..42692)
                     /gene="fldB"
                     /locus_tag="JN691_000616"
     CDS             complement(42171..42692)
                     /gene="fldB"
                     /locus_tag="JN691_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAY7242137.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            42804..43700
                     /gene="xerD"
                     /locus_tag="JN691_000617"
     CDS             42804..43700
                     /gene="xerD"
                     /locus_tag="JN691_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAY7242138.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDLPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            43725..44435
                     /gene="dsbC"
                     /locus_tag="JN691_000618"
     CDS             43725..44435
                     /gene="dsbC"
                     /locus_tag="JN691_000618"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAY7242139.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            44441..46174
                     /gene="recJ"
                     /locus_tag="JN691_000619"
     CDS             44441..46174
                     /gene="recJ"
                     /locus_tag="JN691_000619"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAY7242140.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            46265..47363
                     /gene="prfB"
                     /locus_tag="JN691_000620"
     CDS             join(46265..46339,46341..47363)
                     /gene="prfB"
                     /locus_tag="JN691_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAY7242141.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            47373..48890
                     /gene="lysS"
                     /locus_tag="JN691_000621"
     CDS             47373..48890
                     /gene="lysS"
                     /locus_tag="JN691_000621"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAY7242142.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(48933..49481)
                     /gene="idi"
                     /locus_tag="JN691_000622"
     CDS             complement(48933..49481)
                     /gene="idi"
                     /locus_tag="JN691_000622"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAY7242143.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            49536..49607
                     /locus_tag="JN691_000623"
     CDS             49536..49607
                     /locus_tag="JN691_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242144.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(49604..49729)
                     /locus_tag="JN691_000624"
     CDS             complement(49604..49729)
                     /locus_tag="JN691_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242145.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(49731..51179)
                     /locus_tag="JN691_000625"
     CDS             complement(49731..51179)
                     /locus_tag="JN691_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAY7242146.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            51615..53534
                     /gene="ygfT"
                     /locus_tag="JN691_000626"
     CDS             51615..53534
                     /gene="ygfT"
                     /locus_tag="JN691_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HAY7242147.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQRIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFERHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEQGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            53534..54022
                     /locus_tag="JN691_000627"
     CDS             53534..54022
                     /locus_tag="JN691_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7242148.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDIISAPVMCHQCENAPCVGACPVQALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRLRQQRIVASGEN
                     PL"
     gene            complement(54058..55425)
                     /locus_tag="JN691_000628"
     CDS             complement(54058..55425)
                     /locus_tag="JN691_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7242149.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(55461..>56005)
                     /locus_tag="JN691_000629"
     CDS             complement(55461..>56005)
                     /locus_tag="JN691_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAY7242150.1"
                     /translation="DVYHQYGLTGKNCVFAHCVHLEEKEWDRLSETKSSIAFCPTSNL
                     YLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFNMLQTLNEAYKVLQLQGYRLSAYEAFY
                     LATLGGAKSLGLDDLIGNFLPSKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTL
                     GDDRSIYRTYVDGRLGYERN"
BASE COUNT        13566 a        13968 c        15077 g        13394 t
ORIGIN      
        1 tgctggataa aaactatatc agcgagaatt acattcaggc aattaaagat tccaccatta
       61 acaatggccc ttattatatt ctcgcaccag gcgtggcaat gcctcatgcg cgaccggaat
      121 gtggggcgct taaaaccggg atgtctttga cattacttga acaaggtgtt tattttccgg
      181 ggaatgacga accaattaaa ttactcatcg gactctctgc tgccgatgcc gattcgcaca
      241 ttggcgctat tcaggcgtta agtgaattac tgtgcgaaga agaaatactc gaacaactct
      301 taacagcatc atcagaaaaa caattagcgg acattatcag ccgcggataa ttactctctc
      361 ctctacagga taatattatg gaaaacaagt ctgctcgtgc aaaggtccag gcttttgggg
      421 gatttttgac ggcaatggtc atccccaata taggtgcttt tattgcctgg ggcttcataa
      481 ctgcgttatt tattcccacc ggttggctgc ctaacgaaca tttcgccaaa attgtcggcc
      541 cgatgattac ctgtttattg cccgtgatga ttggttctac aggtggtcat ctggtcggcg
      601 gtaaacgcgg ggcggtcatg ggcggaatag gtactattgg tgtgatcgtt ggcgcagaga
      661 tcccgatgtt ccttggctca atgatcatgg gaccgctcgg tgggttggtc ataaaatatg
      721 tcgataaggc actggaaaaa cgcatacctg ccggttttga gatggttatc aataacttct
      781 cattaggtat cgcggggatg ctcctttgtc tgctgggttt tgaagtgatc ggcccggcgg
      841 tgttaattgc caatactttc gtcaaagagt gtattgaggc gctggtacat gcgggttatc
      901 tgcctctgct gccagtcatc aatgaaccgg cgaaagtgct tttccttaat aatgcgatcg
      961 atcagggcgt ctattatccg ctgggaatgc aacaggcttc ggttaacggt aaatccatct
     1021 tctttatggt ggcctctaac ccaggtccgg gcctggggct gctgctggcg tttaccttgt
     1081 ttggtaaagg gatgagtaaa cgttctgcgc ccggggcgat gattattcac ttcctcggtg
     1141 ggatccacga actgtatttc ccatatgtgc tgatgaagcc gctgactatt attgccatga
     1201 ttgcgggcgg tatgtctggt acctggatgt ttaacctact ggacggtggt ctggtggctg
     1261 gcccaagtcc ggggtctatc tttgcgtacc tggcactgac gccgaaaggc tcgttcctgg
     1321 cgacaattgc cggtgttacg gtaggtaccc tggtgtcctt tgctattact tcgctgatac
     1381 tgaagatgga aaaaacggtg gaaacggaga gcgaagatga gtttgctcag tcagccaatg
     1441 cggttaaggc gatgaaacaa gagggtgcat tctcgttaag cagggttaag cgtatcgcct
     1501 ttgtttgcga tgcggggatg ggctccagtg cgatgggcgc gaccaccttc cgtaaacgcc
     1561 tggaaaaagc ggggctggca atagaagtaa aacattacgc gatagaaaac gtgcctgcgg
     1621 atgccgatat cgtcgtcact cacgccagtc tggaagggcg cgtgaaacgt gtgacggata
     1681 aaccgctgat attgattaat aactatattg gcgatccaaa actcgacact ttatttaatc
     1741 aattaaccgc cgaacataaa cactgattac agaggtaaaa atgaaaacca aagttgctgc
     1801 tatttatggc aagcgggatg tccgtctgcg cgaatttgaa ctgccagaaa ttaccgataa
     1861 tgaactgtta gtgagtgtaa tttctgacag cgtctgttta tcgacctgga aagcggcgtt
     1921 actcggtagt gaacataaac gcgtacccga cgatttagaa aatcatccgg tcattaccgg
     1981 gcatgaatgt gccggggtta ttgtcgaagt gggtaaaaat ctcactggca aatataaaaa
     2041 aggccagcgt tttgtattgc aaccggcgat ggggttacca agcggatatt cagcgggcta
     2101 cagctacgaa tattttggcg gcaatgccac ttatatgatt attcccgaaa tcgccattaa
     2161 tttgggctgc gtattaccgt atcacggctc ttattttgct gcggcgtcgc tggcagagcc
     2221 tatgtgctgc attattggtg cttatcatgc caattatcac accacgcaat atgtttatga
     2281 gcatcgcatg ggcgtcaaac ctggcggcaa tattgcactg ctggcgtgtg cgggtccgat
     2341 gggcattggc gctatcgatt acgccattaa cggtggcata caaccgtcac gggtggtggt
     2401 ggtcgatatc gacgacaaac gcctggcgca ggtgcagaag ctgctgccgg tggatctggc
     2461 ggccagtaaa ggcattgagc tggtgtatgt gaataccaaa gggatgagcg atcctatcca
     2521 gatgctgcgg gcgctgacag gagatgtcgg gttcgatgac atttttgttt atgcggcggt
     2581 gcctgctgtc gttgagatgg ctgacgaatt actggcggaa gatggctgtc tgaacttctt
     2641 tgccgggccg acggataaaa acttcaaagt gccgtttaat ttctacaacg tccattacaa
     2701 cagcacgcac gtagtcggta catccggcgg ttcaacggac gacatgaaag aggcgattgc
     2761 ccttagcgcc acaggacagt tacagccgtc gtttatggtg acccatattg gtggcctgga
     2821 tgcggtgcca gaaaccgtgc tcaatctgcc ggatatccct ggcggtaaaa aactcattta
     2881 taacggcgtg accatgccgc tcactgccat tgccgatttt gccgaaaaag gcaaaaccga
     2941 tccgctgttt aaagagttgg cgcggctggt tgaggaaacg cacggcatct ggaatgaaca
     3001 ggccgagaaa tatctgctgg cacaatttgg cgttgatatc ggggaggccg cgcaatgatg
     3061 tccctggcgt ggccattatt tcgcgttacg gaacaggcag cgttggctgc ctggccgcaa
     3121 accggatgtg gcgacaaaaa taaaattgat ggcctggcgg tcaccgcgat gcgccaggca
     3181 ttaaacgacg tcgcttttcg tggacgagtg gttatcggcg aaggagaaat tgaccatgcg
     3241 ccgatgctgt ggattggcga agaggtgggc aaaggcgatg ggccagaagt cgatatcgcg
     3301 gttgatccca ttgaaggcac gcggatggtg gcgatggggc agagcaatgc gctggcggtc
     3361 atggctttcg ctcctcgaga tagcctgtta catgcgcccg atatgtatat gaaaaagctg
     3421 gttgttaatc gactggctgc cggggcgatt gatctgtctt tgcctctggc ggacaacttg
     3481 cgtaacgtgg cgcgggcgtt aggtaaaccg ctggacaagc tgcgcatggt tacgctggat
     3541 aaaccgcgtc tgagtgccgc gattgaagaa gcaacacagc tgggcgtgaa ggtttttgcc
     3601 ctgccggatg gcgatgtcgc cgccagcgtg ctgacctgct ggcaggataa tccttacgat
     3661 gtgatgtaca ccatcggcgg tgcgccagaa ggcgtgattt ctgcctgtgc cgttaaagcg
     3721 ttaggcgggg atatgcaggc cgagctgatt gacttttgtc aggctaaagg ggattacacg
     3781 gaaaatcggc agattgcaga gcaggagcgc aagcgttgta aggcaatggg cgttgacgtt
     3841 aaccgtgtgt actcgctcga tgaactggtg agggggaacg atatcctctt tagcgctacg
     3901 ggcgtgacgg gcggcgagtt ggtgaacggt atccaacaga tggcgaatgg ggtgcggacg
     3961 cagacattac tgatcggcgg cgcggaccaa acgtgtaata taatagactc cctgcattga
     4021 tggtgatcta tcggaagacc aatggcgacg ctgacagaag atgatgtgct tgagcaactg
     4081 gatgcacagg acaatttatt ttcatttatg aaaactgcgc attctatttt gctccagggg
     4141 atacgccagt ttctgccgtc gctgtttgtc gataacgatg aagagatcgt cgaatatgca
     4201 gtgaagccgt tactcgccca aagcggcccg cttgacgata ttgatgttgc gctgcgtttg
     4261 atttatgcgc tggggaaaat ggataaatgg ctgtatgccg atatcacgca cttttcccag
     4321 tactggcatt acctgaacga acaggatgaa acgcccggat ttgccgatga tataacttgg
     4381 gattttatca gcaatgtcaa cagtataact cgcaatgcga cgctctacga tgcgttaaaa
     4441 gcgatgaagt tcgccgattt cgccgtctgg tcagaggcgc gttttagcgg aatggtcaaa
     4501 acggcgctga cgctggcagt aacgaccacc ttaaaggaat taacgccgtg aaaattgaat
     4561 taacggtgaa tgggctgaac gtgcaagcgc agtaccatga tgaagagata gaacgcgtgc
     4621 ataaaccgtt gctgcgtatg ctggcggcct tacagacggt aaatccgcag cggcgtacgg
     4681 tggtttttct ttgcgctccg ccggggacgg gtaaatctac cctaaccacc ttctgggaat
     4741 atctcgcgca acaagacccg gagctgcccg ctattcagac gctcccgatg gatggttttc
     4801 accattacaa tagctggctg gacgcgcatc aattgcgccc cttcaaaggc gcaccagaga
     4861 catttgacgt tgcgaaactg gcggaaaatc tgcgccaggt ggtggaaggg gattgtacgt
     4921 ggccgcagta cgatcgacaa aagcatgatc ctgttgaaga tgcgttgcac gttaccgcac
     4981 cactcgtcat cgtcgaagga aactggttgt tgctggacga cgagaagtgg tgccagcttg
     5041 cgcaattttg tgatttctcg atctttatca acgcgccagc tactgcgttg cgggagcgtc
     5101 tggtggggcg caagctggcg ggaggggtat cgctggcaga tgcagaagcc ttttatgacc
     5161 gtaccgacgg gccgaatgtt cgccgggtgc tggaagagag tctgcctgca aatttgacct
     5221 taatgatgac cgctacgggt gaatatcgtc tggtggatta acgttcctga ttacaccatc
     5281 ttccagtcgc accacacgag agaaatgacg ccgggtaaat gcggcgtcat gactgattgc
     5341 taccacgctc gtgcctcgct ggcggcactt ctccagccag ctttcaaaca cgctcaacca
     5401 attttcgtca aaatcccgac tcggctcgtc cagcagtaat agtggcggag agagtgcttc
     5461 caggcaggcg acggcgacca tccgtcgctg cgcactatgc agatccaacg ggtgcgagtt
     5521 ggcagtgtct gctaactgac aacattgcag ggcagcatgg gtacgttgag tgatttcatc
     5581 cgccgggcat ttttgcagtt tcaaaccaaa tgcgatctcg tcagcgacgg tgctgtgaaa
     5641 aagttggttt tccgcttcct gaaacagtac gccaaccttt gccgcgcgct ggcggttttt
     5701 caggtttttc atcgcctgtt gttgcagcat aaccgtaccg gctgtgggcg taagaagacc
     5761 tgccattact cgtaacagcg tcgatttccc tgcgccattg tcaccggtca gcgccaacca
     5821 ttcaccttgt ttgagttgca gagaaatgtc gcaaagacaa tccgttgccg cgccaggcca
     5881 gcgataagat atcttattta acgttaacat agtggctgga gcgctccgtt ttgcaattgc
     5941 cacgcttgct ggcagtagtt caggaaaggt gtgcgatgac gttcaaaaag aataatcagc
     6001 gaaccacgtt caaacgccca gtgttgtaag cgctgcaaca gcatttcgct ggcctgcggc
     6061 gtcaggcggc taaaggcttc atcaagaatt aagagctttg gctgcatcgc gatggcgcag
     6121 gcgatgacca cgcgctgtgt ctctccaccg gagagtgttg ccgggtgacg atggcgcaac
     6181 ggctggcact cggtcagagt cagggcggca tcaatccgcg ccataatctc tgcttcagca
     6241 agacacagat tttctggccc aaaggcaact tcttcttcca cgctaaaggt acagccggag
     6301 agctgcaaat agggcgattg ctggacgagc tggatggtgg cggattgttc attaagcggg
     6361 agatgcccaa tgggcgtacc gaggagcgtc cccgtgcctg ttatctctct tggcagaaag
     6421 tccggatacc agcccgccat cagctgtgcc agcgtacttt tgccactgcc gttgtcacca
     6481 aagatggcga ccatccccgg tttgacatag tgaaagtcat aacagaacgg aggatgcgtt
     6541 gagtgtgtcg ggcaataacg gaactgttct aacgtaacca tatccacgct ccgaattctg
     6601 tcagcatcag caagatcatg gtgtaacgcg ctacgcgttg tagggtgcta tcggctggtg
     6661 cccacaacgt tgttcgtcgg ttgtggatcc gaaatgcccg catatccagc gccgcaccac
     6721 ggacagccag atcgttaagc gcattgtgtg ttagcggaat aattaacgcg ggcatagcac
     6781 gtaaacgctg ataccagccc tcatccagcg gcacgccgcg ggcacgctgc gcttcatgaa
     6841 taatcgctaa ttgccgtttt aactgctcaa cgaccaataa cggcccggca aacagataag
     6901 cgacgcctgg cggcaggcga gaagcaaaca gagcgcgaat aaaacattgc acggggacgt
     6961 actgcattca caactgtgag gttgaaacaa tcgccagaat cagcaaccag agcgtaatag
     7021 catgcaacca ccgttctggc gagcgtggcg tgccgcttag ccattctggt agccagccac
     7081 cgtgtaccag ccacagcccg gctcccagcg agaacatcag ccagacgaca tattttgccc
     7141 gccgccgcgt ggcctttagc gcaataaggc agaaaaaggt cgccgcgcta tagatcggca
     7201 aaatggtctg cgcgggcagg ataagcgtgg tgcaggccgc caatgcccac agtgttaatg
     7261 aggtaaatgg atgcatttag cgcacggcag ccattgccgg aaaatggcgc gtggtgcgaa
     7321 tcggcagctg gcgcagtaaa agccagacaa tcaccgctgt aagaatttta tcgaccaggt
     7381 tcgcgcctat caccgtaatc gccaccgact ccaccaggtt ttgccccatc gagtgcatcc
     7441 aggcaacgaa caaatcggct ccgctaccgg tgacaccgcc gaacagcgca gtacgcagcg
     7501 gtacagcgac gaccgttacc gcaagcgtaa taatcacgcc gctgacaacg acttttggca
     7561 gggtgcgaaa ccaacccgct cgcgccagcc aaccggcgac caggccgatg accatcgcga
     7621 cgggtgcaaa tgcggcggcg atcgggtcgg tcagcaatcc ccagagtaaa ttggtcagta
     7681 agccggtcag cattccgatc accggaccaa gcagtaccgc gctaataagt gtgccgatgg
     7741 agtcgagaaa tataggtaat ttgaccatgc tggccagttg cccgccgatc atattgatgg
     7801 cgatagatat aacgatcaac accagtgcct gactggaaaa gtggctacgc gccataagaa
     7861 aacctctttg aaagtagtta tttaacgggg gaaatatgca actcgtcgta accagaaagc
     7921 gcgcaaggtt ggcggctaaa cgccagttcc tcaaggctac cgatcaccag ttcaagcgtg
     7981 gtccgcacgg tacagccggg catcatatga ccgccaagca tcgtgccgtg tggatcggaa
     8041 acgcagagat gaagatgctc accactctgt tccagcgtgc cgtttagtga gatcacttcg
     8101 aatgtaccgt tcaggagcgt ggtgccttct tgcccggcat agcgcaaggc gacgtcagtc
     8161 aggctaccgg tacagcctgc aatccaggcg gcatgaagtt gatgttgctg tgcaaaagcg
     8221 tgtagctggg aaaaaacttc ctgcccgggg aggaggcgca gtgcgtaaaa gcgtgcggag
     8281 gaagaatatg ggtagggcaa tgtcatcatt ggagtactcc gaaactcgga cgtattgcgg
     8341 ataatatcga cgctgcatca gtcatattct gatataagtc agggtttgtg acggcacaac
     8401 gaaaagattt tgctacgctt attcgtgatt ctgcaaagtg gaataaattc cacaggggcg
     8461 gggctgaaaa cgtgacgtaa gtcagataat tgctttcttt gactggacaa tcattccttt
     8521 tattccacgt ttcgcttatc ctagctgaag cgtttcagtc gattaaatgt tcgacaatta
     8581 accaatcagt cgcagtttgc gacaggtaag gtttccccgg acgatttgct ggattactct
     8641 gtctggcaaa tttgctcagg gaaaaccttg caggagatct atgaccgtac gcgtagcgat
     8701 aaatggcttc ggtcgcatcg ggcgtaatgt ggttcgtgct ttgtatgaat ccggacgccg
     8761 ggcggaaatt accgtggtgg caatcaacga actggcggat gctgcgggca tggcgcattt
     8821 gttgaaatat gacaccagcc atggccgttt tgcatgggaa gtacgacagg aacgcgatca
     8881 actttttgtt ggtgatgacg ccatccgcgt attgcatgaa cgttcactgc aatcgctccc
     8941 ctggcgtgaa cttggcgttg atgtagtcct cgactgcacc ggcgtatatg gctcccgcga
     9001 gcatggcgaa gcgcatattg ccgccggggc caaaaaagtg ctcttttcac atcctggcag
     9061 taacgatctc gacgcgaccg ttgtttacgg cgtcaatcag gatcaacttc gtgcggaaca
     9121 ccgcatcgtt tctaacgctt cctgtaccac gaattgcata attcccgtca tcaaattgtt
     9181 agatgatgcg tacggtattg agtccggcac tgtgaccaca attcactccg ccatgcacga
     9241 tcaacaggtt attgatgcat accatcctga cctgcgtcgc acccgggcag ccagccagtc
     9301 gatcattccg gtcgatacta aactggccgc tggtatcaca cgattttttc cgcaatttaa
     9361 cgatcgcttt gaagcgattg cggtacgtgt gccaaccata aatgtgacgg caatcgattt
     9421 aagcgtgacg gtgaagaaac ctgtaaaagc caatgaagtc aacctgttgc tgcaaaaagc
     9481 agcacaaggt gcatttcatg gtatagttga ctatacggaa ttgccgttgg tctctgtaga
     9541 ttttaaccac gatccgcaca gtgccattgt cgatggcacc caaacccggg tcagtggcgc
     9601 acacctgatc aaaacgttgg tctggtgcga taacgaatgg ggctttgcta accgaatgct
     9661 cgacacgacg ttagctatgg ctactgttgc tttcaggtaa gacgcaagca gcgtctgcaa
     9721 aacttttaga atcaacgaga ggattcacca tgtctgtaat taagatgacc gatctggatc
     9781 ttgctgggaa acgtgtattt atccgtgcgg atctgaacgt accagtaaaa gacgggaaag
     9841 taaccagcga cgcgcgtatc cgtgcttctc tgccgaccat cgaactggcc ctgaaacaag
     9901 gcgcaaaagt gatggtaact tcccacctgg gtcgtcctac cgaaggcgag tacaacgaag
     9961 aattctctct gctgccggtt gttaactacc tgaaagacaa actgtctaac ccggttcgtc
    10021 tggttaaaga ttacctcgac ggcgttgacg ttgctgaagg tgaactggtt gttctggaaa
    10081 acgttcgctt caacaaaggc gagaagaaag acgacgaaac cctgtccaaa aaatacgctg
    10141 cactgtgtga cgtgttcgta atggacgcat tcggtactgc tcaccgcgcg caggcttcta
    10201 ctcacggtat cggtaaattc gctgacgttg cgtgcgcagg cccgctgctg gcagctgaac
    10261 tggacgcgct gggtaaagca ctgaaagaac ctgctcgccc gatggtggct atcgttggtg
    10321 gttctaaagt atctaccaaa ctgaccgttc tggactccct gtctaaaatc gctgaccagc
    10381 tgattgttgg tggtggtatc gctaacacct ttatcgcggc acaaggccac gatgtgggta
    10441 aatccctgta cgaagctgac ctggttgacg aagctaaacg tctgctgacc acctgcaaca
    10501 tcccggttcc gtctgatgtt cgcgtagcaa ccgagttctc tgaaactgca ccggctaccc
    10561 tgaaatctgt taacgatgtg aaagctgacg agcagatcct ggatatcggt gatgcttccg
    10621 ctcaggaact ggctgaaatc ctgaagaatg cgaaaaccat tctgtggaac ggtccggttg
    10681 gcgtgttcga attcccgaac ttccgcaaag gtactgaaat cgtggctaat gctatcgcag
    10741 acagcgaagc gttctccatc gctggcggcg gcgacactct ggcagcaatc gacctgttcg
    10801 gcattgctga caaaatctcc tacatctcca ctggcggcgg cgcattcctc gaattcgtgg
    10861 aaggtaaagt actgcctgca gtagcgatgc tcgaagagcg cgctaagaag taaaaaatca
    10921 cagggcaggg aaacctgccc ttgtttcagc gcgcttttag agcacgcatt ttttcatact
    10981 ctaaataatt cgagttgcag gaaggcgaca agagagtgaa tccctaggag cttacataag
    11041 taagtgactg gggtgagcga acgcagacgc agcacatgca acttgaagta ttatgagtat
    11101 aaggcccgac gatacaggac aagagacatg tctaagattt ttgatttcgt aaaacctggc
    11161 gtaatcactg gtgatgacgt acagaaagtt ttccaggtag caaaagaaaa caacttcgca
    11221 ctgccagcag taaactgcgt cggtactgac tccatcaacg ccgtactgga aactgctgct
    11281 aaagttaaag cgccggttat cgttcagttc tccaacggtg gtgcttcctt tatcgctggt
    11341 aaaggcgtga aatctgacgt tccgcagggt gctgctatcc tgggcgcgat ctctggtgcg
    11401 catcacgttc accagatggc tgaacattat ggtgttccgg ttatcctgca cactgaccac
    11461 tgcgcgaaga aactgctgcc gtggatcgac ggtctgctgg acgcgggtga aaaacacttc
    11521 gcagctaccg gtaagccgct gttctcttct cacatgatcg acctgtctga agaatctctg
    11581 caagagaaca tcgaaatctg ctctaaatac ctggagcgta tgtccaaaat cggcatgact
    11641 ctggaaatcg aactgggttg caccggtggt gaagaagacg gcgtggacaa cagccacatg
    11701 gacgcttctg cactgtacac ccagccggaa gacgttgatt acgcatacac cgagctgagc
    11761 aaaatcagcc cgcgtttcac catcgctgcg tccttcggta acgtacacgg tgtttacaag
    11821 ccgggtaacg tggttctgac tccgaccatc ctgcgtgatt ctcaggaata tgtttctaag
    11881 aaacacaacc tgccgcacaa cagcctgaac ttcgtattcc acggtggttc cggttctact
    11941 gctcaggaaa tcaaagactc cgtaagctac ggcgtagtga aaatgaatat cgataccgat
    12001 acccaatggg caacctggga aggcgttctg aactactaca aagcgaacga agcttatctg
    12061 cagggtcagc tgggtaaccc gaaaggcgaa gatcagccga acaagaaata ctacgatccg
    12121 cgcgtatggc tgcgtgccgg tcagacttcg atgatcgctc gtctggaaaa agcattccag
    12181 gaactgaacg cgatcgacgt tctgtaagtc attcctttct gcttatctca aggcccgctc
    12241 tgcgggtctt tttttcgcca aaagcaaggt gattcagatg agaaatctgt gatctatttg
    12301 gcaaaattat gctttattgt ttacccttgt cagactgccc gtcataaggc ggcggagtgt
    12361 atttctccat tttgagtcag ttgaaaagga atattgaatg gaagatttga atgttgtcga
    12421 tagcataaac ggcgcgggaa gctggctggt agctaaccag gcgctgctgc taagttatgc
    12481 agtaaacatc gtggcggcac tcgcgatcat catcgttggt ttgattatcg cgcggatgat
    12541 ttccaacgcg gtgaatcgcc tgatgatctc ccgtaaaatc gatgccactg ttgctgattt
    12601 tctttctgca ttagtccgtt acggtattat cgcctttacg ctaatcgctg ccctgggacg
    12661 cgtgggtgta caaacagcgt cagtcattgc tgtactcggt gccgcaggct tagctgttgg
    12721 tctggctttg caggggtcac tttctaacct ggccgctggc gtgttacttg tcatgttccg
    12781 cccgttccgt gccggagaat atgttgacct cggcggcgta gccggtactg tgctgagtgt
    12841 gcagattttc tccaccacca tgcgtactgc agacggtaaa attatcgtta ttccgaacgg
    12901 taaaattatt gccggaaata ttattaactt ctcccgcgag ccagttcgcc gtaacgaatt
    12961 tattattggc gtggcgtatg attccgatat cgatcaggtt aagcagatcc tgaccaatat
    13021 tatccagtct gaagatcgca ttttgaaaga tcgcgaaatg actgtgcgcc tgaacgaact
    13081 tggtgcatcg tcgattaatt tcgtggtccg cgtctggagc aacagcggcg atctgcaaaa
    13141 cgtgtactgg gatgtgctgg agcgtattaa acgtgaattt gatgccgccg gtatcagctt
    13201 cccgtacccg caaatggatg tgaactttaa gcgggtgaaa gaagacaaag ctgcgtaatc
    13261 aacgctgagg ccagataata ctctctggcc tctctcttat tagttattct gattgccaat
    13321 taatattatc aatttccgct aataacaatc ccgcgatata gtctctgcat cagatactta
    13381 attcggaata tccaacgtgt tttcttatta ctttcaaggt cttgctcttg gggcagctat
    13441 gattctgccg ctcggcccac aaaatgcttt tgtgatgaat cagggcattc gtcgtcagta
    13501 ccacattatg attgccttac tttgcgctat cagcgatttg gtcctgattt gcgcggggat
    13561 ttttggtggt agcgcgttat tgatgcagtc gccgtggttg ctggcgctgg tcacctgggg
    13621 cggcgtagtc ttcttgctct ggtatggttt tggcgctttt aaaacagcga tgagtagtaa
    13681 tattgagtta gctagtgccg aagtcctgaa gcaaggcaga tggaaaatta tcgccaccat
    13741 gttggcagtg acctggctga atccgcatgt ttacctggat acttttgttg tactgggcag
    13801 ccttggcggg cagcttgacg tcgaaccaaa acgttggttt gcgctcggga caattagcgc
    13861 ctctttcctg tggttctttg gtctggctat tctcgcagcc tggctggcac cgcgcctgcg
    13921 cacggcaaaa tcacagcgca ttatcaatct ggtagtggga tgtgtcatgt ggtttatagc
    13981 cttgcagctg gcgagagacg gtattgctca tgcacaagcc ttgttcagtt aggcgctatc
    14041 tgatggaaaa ataaaacaga ggcgctaagc ttgcctccag aggtcctgaa ttttgggcaa
    14101 gtcaattaac acggagagac taacgtgaag ttcaaagtta tcgccctggc ggcattaatg
    14161 ggtattagcg ggatggcagc gcaggctaac gaattgccgg atggaccgca tattgtcacc
    14221 tccggtacgg caagtgtgga tgcggtgcca gacattgcca ctcttgcgat tgaagttaac
    14281 gtggccgcga aggatgccgc tacagccaag aaacaggcag atgagcgcgt cgcacaatac
    14341 atttccttcc ttgaactcaa tcagatcgcg aaaaaagata tcagctcagc gaatttacgc
    14401 acccagccag attatgacta tcaggatggt aaaagtatcc tgaaaggcta ccgagctgtg
    14461 agaacggtgg aagtcacgct ccgtcagtta gacaaactga attccttgct ggatggcgcg
    14521 ctgaaggcgg gtcttaacga aattcgttct gtgtcgctgg gcgtggcgca gccggatgcc
    14581 tataaagaca aagcgcgtaa ggcagcgatt gataacgcga ttcatcaggc gcaggaactg
    14641 gcgaacggct ttcatcgtaa actggggccg gtatatagcg tgcgctacca tgtttccaac
    14701 tatcagccca gcccaatggt gcggatgatg aaagccgatg ccgcgccggt gtctgcccag
    14761 gaaacttacg agcaggccgc tattcagttt gatgatcagg tcgatgtggt cttccagtta
    14821 gaacctgtgg atcaacaacc cgctaaaaca cctgcagcac aataatactt gtagcccgac
    14881 gtattcgtcg ggctaatgcc tttacccgat attgcctcct ttttctgtaa ttttctgtaa
    14941 aaccatgacg taatcaccta tctttgctcg cggtttcatc aaggataggc tgactgaaac
    15001 ggttaagcgg acaaggatgc ggggaggggg gatggacatt tttatttcga aaaagatgcg
    15061 caatttcatt ttattagcgc aaaccaataa tattgcccgg gcggcagaaa aaatccatat
    15121 gacggcttcg ccatttggta aaagcattgc cgcgctggaa gaacaaattg gctatacgct
    15181 atttacccgc aaagataata atatcagcct caataaagcc ggtcaggaac tgtatcaaaa
    15241 actgttcccg gtttatcaac gactttctgc gattgataat gaaatccata actcagggcg
    15301 tcgttcacga gaaattgtga ttggcataga taatacttat ccgacaatta ttttcgatca
    15361 gttgattagt ctcggagata agtatgaagg cgtgaccgcc cagccagttg agttcagcga
    15421 aaacggcgtt atcgataatc tttttgaccg tcagttagat tttattatct ccccacagca
    15481 tgtatctgca cgagttcagg aactggaaaa cctgactatc agtgaattac caccgctgcg
    15541 attgggcttt ctcgtttctc gccgttatga agagcgtcaa gagcaagagc tgcttcagga
    15601 gctgccctgg ctacaaatgc gctttcaaaa ccgggctaac ttcgaagcaa tgattgatgc
    15661 gaatatgcga ccttgtggga tcaatctcac cattatttat cgtccctaca gttttatggc
    15721 caaaatcagc gccgtggagc gcggacattt tctgacggtg atcccgcatt ttgcctggcg
    15781 actggtgaat ccggcaacgc tgaaatattt tgatgcgccg cacaggccga tgtatatgca
    15841 ggaatatctt tattcaatca gaaatcatcg ttataccgcc acgatgcttc agcatattgc
    15901 tgaagatcgt gacgggacaa accattaacc cagcatcgag ccggttgcaa ttaaattacg
    15961 gtgtaagtcg aagacgtggc taagatcgtg gtgaatatgt ccgccgggcg cattttccag
    16021 atagcgatgc agataatccc gatacatagg atgtgcacaa ttatcaatga tagtgcgggc
    16081 gcgttgaagc ggggaaagac cgcgcagatc ggcgatccct tgttcggtga tgatcacttt
    16141 gacgctgtgt tcgctgtgat caacatggct gcacattggc acgacggttg agatcttgcc
    16201 ttctttagca atcgacggag ccataaagat cgacagatac gcgttgcgtt caaaatcacc
    16261 gctgccgccg atgccgttca tcagatcgac cccggctacg tgcgttgaat ttgcatgccc
    16321 gtaaatatca aactccaggc cgacgttcag agcgatgacg cccagacgac ggatgatttc
    16381 cgggttattg gaaatctcct gcggacgcaa cacaatgcgg ctggcaaagt aatccatatt
    16441 gttgtaaatc ttgcgcaggg aatcggccga gattgtcagg ctggaggcgc tggccccgct
    16501 gattttgccg gtttccagta aatgcaccac cgattcctgt agcacttccg aatacatcat
    16561 gaacggagga atttccgggt tttcccccag acgcgccatt accgcattat tgatattgcc
    16621 cacgccactt tgcagcggca gaaattccgg cggaatacgc ccatgcgcca tttcctgcaa
    16681 taagaacgtg accacgttat cggcaatctg ctggcacatg ggattttgct tatccagcat
    16741 attaccggcg tcgggcaagt tggtttccac gacggcgaca atctttttcg gatcgatttg
    16801 cacatagcgg gtaccgacgc gatccattgc atggaagatc gacacgctat tgcgccgcgg
    16861 tggcgcgcca ggaatcacaa tatctgccag ttctgcaacg cgcggatcgt gatagtgatt
    16921 aagttcaatg atcactttct tcgctcgcag cagccaggtc ggcgcattac cgatcccgct
    16981 ggttaaccag actcgaccat ccggtgccag tgccgatgct tcaatgacgg caacatcaat
    17041 gtcgccgaag aaaccgtaat tgaccatttg cgccacttcg ctcaaatgca ggtcaacgaa
    17101 actcaccgcg ccctgattga tctttttacg taaactggac gatgtttgat atggcgcacg
    17161 ccaggaaaca gcatcggcgt cagaaagtac atcgtcagcg gcggcgctga ttgacgcccc
    17221 cgtcagcagg cgaatttgat acggcttttt ggcctcatgc tgttcgttag ctctgcgggc
    17281 aatcgcggcg ggtagggctt tcggcgaacc cgctggggta aagccgctaa atgccaccat
    17341 gtcgttatgc tggataattt ctgccgcttc atcggcggtc atccttgtcc actgagtttc
    17401 catttttact ccttcgttcg cagggattaa tgaccaacga aattaggttt acgtttttcg
    17461 aggaaagcgt tcatcccttc ctggtaatct tcgctgtcat aaaccgcgcg gcgcatcccc
    17521 tgaatacgtt caaattcatc ggagttcatg gtgtgtgctt cgcccagtac acgcagctct
    17581 tctttgataa cggcaatggc taacggcgct ttctcagaga tgtggtgcgc catttgtaag
    17641 gtgaaatctt ccagttcttc cacttccaca acatggttga ggatgccgac agccagcgcg
    17701 cgctgggcgg tgattggcga agcggtaaaa atcagctctt tgacaatgtg gaagcccgcg
    17761 tcgcgggtca ggttgtgaat gccgaccagg ttatacggga cgccgaggtt tacaggcgtc
    17821 attgagaagg ttgaggtact ggcggcgatg atcagatcgg aactcatgat catttcaaat
    17881 gcgccacccc aaacactacc ttccaccatc gaaatgatcg gtttcgggaa tttttggatc
    17941 atgcgggtga tttgacgcaa tggatcatca taggagagcg gatcgcgacc gccagacggc
    18001 agttcgtgaa tatcgtgacc tgcggagaag actttggagc cacttggcgc ccgcaaaatg
    18061 atacagcgaa tttccggccg gttgagatcg cttaacgcct gcataagatt atcaataaag
    18121 actttactta aggcattaag ttttcgtcca tagttaaact caatgaccgc cactttgttg
    18181 atagtggcaa cgttcacata ctgataagac ataaaaattc ctttaatcaa aatattgcgt
    18241 ctggataaat tcactgagct gccgcaggcc ggtgcgcggt gagagcgtat tgtttttgac
    18301 cgctaaaagc gtctggcggt aatagcgatc gaaatcttca ttcgcgaaca ggtgattcag
    18361 tacttcttct tcggtctgct tacgcagcca ttccaccgat tgttgttgcc gcacttgttg
    18421 taaacgacca ctggcagtta gcgcggtttt gaagtcgatg atggcgtgcc agatctcatc
    18481 gattccacgt ttttccagtg cgctacaagt cagaccccgt ggctgccatt cgtcgtattt
    18541 acgtcgcaga atatgcaggg cactctcgta catatgccgg gcaatggcga cattggtatg
    18601 gttatcgcta tcgtctttgt tgataacgag cagatcagcc acttccatca gccctttttt
    18661 aatgccctgc agatcatcgc cgccaccggc aatttgcaac gagataaaac agtccaccat
    18721 gcgggcgact tccgtttccg actgcgcgac gccaaccgtt tcgacaatca ctacgtcata
    18781 acccgccgct tcgcataaca gcattaattc ccgtgctcgc tgactggcac cgcccagatg
    18841 accggaggag ggtaccgggc gaataaacgc cgcctcggca cgcgccaggt cattcatgcg
    18901 ggttttatcc ccgagaatgc taccgccagt gaccgggctg ctgggatcga ccgcaataac
    18961 agcaaccttt aatccctcac gaatcaacaa catgccaaag gcttcaagaa aggtactttt
    19021 ccccgcgccg ggtgtgccgg taacgcccag tcgcagggcg ttaccacaga acggcataat
    19081 ggcgtcaagc agctgtgtac ttagcgcctg atgacgtggg tgacggcttt ccaccagcat
    19141 catggcttgg gcgagcgtgg cacgctcacc ctgacgtaag cggcgaatac tttctgccag
    19201 cgtggcttca ttaatcatga tgctggctta tcagattcag tacgtcgcgc acactgtcga
    19261 gcataggtgt acctggacca taaatcgccg ccacgccgcg ctcttgcagg aaggcgtaat
    19321 cctgcggcgg aatgacgcca cccgcgacca cgcagatatc ttcgcgtccc cattttttca
    19381 gcgcttcgac cagttccggg atcagcgttt tatgaccggc agccagtgag gatgcgccca
    19441 ctacgtgaac gtcgttttct acggccaggc gggcgatctc ttcaggtgta gagaacatcg
    19501 ggcttaaatc tacgtcgaaa ccgagatcgg aataggcgct ggcgatcact ttcgcgccgc
    19561 gatcgtgtcc atcctggccc atcttagcga tcagaatgcg cgggcgacga ccattgtcgg
    19621 caaggaactg ctccgtttgc gcaacaatgg catcgaactc ggaggccgat ttctcagact
    19681 gatgatagct ttgcgcaatc acgccggtaa cacactggca ccagataacg gtcgaaagcg
    19741 acttccagcg catcggaaat ttcacccagg gtggcgcgaa cgcgagcggc attaacagcg
    19801 gcagccagca ggttttcgtt atgctgtgcg gcgtgagtca gggcgttcaa cgcggcggtt
    19861 acggcggcat catcacgggt ggcgcgaatg cgttccagcg aagcaatttg ctcgttacgc
    19921 accatcacgt tgtcgatctc aagtacatcg gtttcgtctt cgtgatccag tttgtacttg
    19981 ttgacaccaa cgatgacacg cttgccctgg tcgatcagcg actgttcgcg cgctgaggcc
    20041 tcttcgatca ttcgttttgg cagacctgct tcgatcgctt tcgccatgcc accggcttcg
    20101 tcgatctgtt ggataatagc tctggcttgt ttgacgattt gatcggtcag cgactcaatg
    20161 taataggatc cggccagtgg atcgacggtg tggcagagtt ctgattcttc ctggatgatg
    20221 atctgggtgt tgcgggcaat gcgtgctgag aaatcggtag gcaaaccaag cgcttcgtca
    20281 aaggcgttgg tatgcagtga ctgagtaccg cccagcgtcg cagccagtgc ttcaatggtg
    20341 gtgcggataa cgttgttata cggatcctgt tcagtcaggc tccagcctga ggtctggcag
    20401 tgggtacgca gcgccagtga tttcggatcc tgtgcgccaa atccactgac cgcttcgctc
    20461 cataaataac gtgccgcacg caacatggcg acgttcataa acagatccat gccgataccg
    20521 aagaataacg acaggcgagg agcgaagtca tcaattttca gtccggcaga gattgctgct
    20581 ttgatgtact caatcccatc ggcgagcgta aatgctacct gctgcacgca gttggcaccc
    20641 gcttccccca tgtggtaacc gctgatactg atggtattaa atcgcggcat gttgccggaa
    20701 caccaggcga tgatgtcggc gataatgcgc attgacggtt ttggtgggta aatataggtg
    20761 ttgcggcaga ggtactcttt gagaatatcg ttttgaatgg tgccggtcag tttatcaggt
    20821 gtaacacctt gctcttctgc ggcgacgata taaaacgcca gtactggtag cactgcgcca
    20881 ttcatggtca tcgaaaccga cattttatcc agcgggatct ggtcgaacag gactttcata
    20941 tcttccacgg tgtcgatagc gacgcccgct ttgccgacgt cacccgccac gcgtgggtta
    21001 tcggagtcgt agccacggtg ggtggcaagg tcaaacgcaa cggaaagacc tttttgcccg
    21061 gcggccaggt tacggcgata aaaagcgttg gactcttttg ctgttgaaaa accagcatac
    21121 tgacggatgg tccacggttg ggcggtatac atagtggcac gcgggccacg aacgtagggc
    21181 ggcaaaccag gaagggtacc tgtcacctcc agattatcga gatcggcttc ggtatacagc
    21241 ggcttgatgg cgatcccttc cgcggtttgc tgaaccagcg agtcgacagt tttctcccga
    21301 cggctcaatt ccttgttggc aagctgttgc cactcctgct cgttagacat tatttgctcc
    21361 gtaaaacgca atcaaaatga gggtatttgc gcttacggtg agctttttac gtgggtttgt
    21421 cgccagcaaa aaaaatgcat tcagacgtcg ttttttgccg cctgagattt gtctattttg
    21481 tgatgtgatt caaggaatac aaaaagaagt gaggacaacg cctgatatgt gctatcaggc
    21541 attatttgat ggattaatcc tgacgaagga ctttgtgacc ataatcgagt aacgcatcag
    21601 tgactttacg catcatgcgg ctttcaggag caaagcggtg ccagtagagc atccgtcgtt
    21661 gaaatagccc aggcgttaag tcaatcagtt caccgctggc cagctctttc tcgatttgca
    21721 ggtgcgggat catacagcag gtggtgccct ggcgagcaag ttgtacgaac gcttctgaag
    21781 aattaacgat atggcagggt acgctgccag gaggaagatc gaagttttgc tgcaaaaagg
    21841 cctggtgcat atcgtcaaga tggtcaaacg cgaccactgg cgctttcaat aatgccgaac
    21901 gcgttacgcc gttaggaaaa tatttttcgg caaacggttt tgagctgacg aacagatagt
    21961 cgagcgcacc gagtttatcg acaagacaac tcggcagcgc ctgatgttga atactcaccg
    22021 cgccgaccac ttcgccgcgg cgcagacgtt cctgagtacg ggtttcatct tctacctgca
    22081 agttgaggcg gataggcgaa tcagccaaca caggagccag tgccggaagc aaccacgtcg
    22141 ccagactgtc ggcgttgacc gccagtgaaa gcagcagcgg agtcgaaccg gtttgttcat
    22201 cgcccagcca ctcttcttcc agcaactcca cttggcgcag cagtgccagc agtttttgcc
    22261 cttgttccgt cgggcgcggc ggtacggtac gcaccaacag cggctgcccg aacatatttt
    22321 ccagttgctt aatgcgctgt gagacggctg attgtgtaat gcacagcttt tgtgccgcgc
    22381 gctcaaatcc tcgttcacgt atcaccgcat ccagtgcctg taatgttctg tagtccgggc
    22441 gtttcattgc tgttgtctgc tcctggtaaa tgctttttcc tgcactatga cacaattttg
    22501 tgtcaggttg catataccga agccgtcggc gggtaacatt tgtatcaatg gttaaacgcg
    22561 cagcttaaaa gatcagggat ttgcgctatt gcatttcgct tagcgacttc gcgccctggt
    22621 tatgttgaat ggcgtggcgt tattgcctct gttcgcaccg tggagcggca tacccagacc
    22681 gttgtatgct ttatttgcgg ggcgaaaggg gatgcccgcc attgcgcgat attttatgga
    22741 tgagttaacc acccggcttg ccaacggggt ctgaatcgct ttttttgtat ataatgcgtg
    22801 tgaaatttca taccacaggc gaaacgatca tgacgcagga tgaattgaaa aaagcagtag
    22861 gatgggcggc acttcagtat gttcagcccg gcaccattgt tggtgtaggt acaggttcca
    22921 ccgccgcaca ctttattgac gcgctcggca cgatgaaagg ccagattgaa ggggccgttt
    22981 ccagttcaga tgcttccact gaaaaactga aaagcctcgg cattcacgtt tttgatctca
    23041 acgaagtcga cagccttggc atctacgttg atggcgcaga tgaaatcaac ggccacatgc
    23101 aaatgatcaa aggcggcggc gcggcgctga cccgtgaaaa aatcattgct tcggttgcgg
    23161 aaaaatttat ctgtattgcc gacgcctcca agcaggttga tattctgggt aaattcccgc
    23221 tgccagtaga agttatcccg atggcacgta gtgcagtggc gcgccagctg gtgaaactgg
    23281 gcggtcgtcc ggaataccgt cagggcgtgg tgaccgataa tggcaacgtg atcctcgacg
    23341 tccacggcat ggaaatcctt gacccgatag cgatggaaaa cgccataaat gcgattcctg
    23401 gcgtggtgac tgttggcttg tttgctaacc gtggcgcgga cgttgcgctg attggcacac
    23461 ctgacggtgt caaaaccatt gtgaaatgat ctgacggggg aacctccccc gttaaaaaaa
    23521 ttctcttcat taaatttggt gacatgtgtc acgcttttag caggcaattg tcgattgctc
    23581 taaataaatc ctctaaacca gcatattcat ccaagaatta cctttgcgtg atatttcctc
    23641 aacatcgcga cgcaaacgtt catattgccg caatattatt ttttgatatg ttgaaaggcg
    23701 gatgcaaatc cgcacacaac atttcaaaag acaggattgg gtaaatggca aaggtatcgc
    23761 tggagaaaga caagattaag tttctgctgg tagaaggcgt gcaccaaaag gcgctggaaa
    23821 gccttcgtgc agctggttac accaacatcg aatttcacaa aggcgcgctg gatgatgaac
    23881 aattaaaaga atccatccgc gatgcccact tcatcggcct gcgatcccgt acccatctga
    23941 ctgaagacgt gatcaacgcc gcagaaaaac tggtcgctat tggctgtttc tgtatcggaa
    24001 caaaccaggt tgatctggat gcggcggcaa agcgcgggat cccggtattt aacgcaccgt
    24061 tctcaaatac gcgctctgtt gcggagctgg tgattggcga actgctgctg ctattgcgcg
    24121 gcgtgccgga agccaatgct aaagcgcacc gtggcgtgtg gaacaaactg gcggcgggtt
    24181 cttttgaagc gcgcggcaaa aagctgggta tcatcggcta cggtcatatt ggtacgcaat
    24241 tgggcattct ggctgaatcg ctgggaatgt atgtttactt ttatgatatt gaaaacaaac
    24301 tgccgctggg caacgccact caggtacagc atctttctga cctgctgaat atgagcgatg
    24361 tggtgagtct gcatgtacca gagaatccgt ccaccaaaaa tatgatgggc gcgaaagaga
    24421 tttcgctaat gaagcccggc tcgctgctga ttaatgcctc gcgcggtact gtggtggata
    24481 ttccggcgct gtgtgatgcg ctggcgagca aacatctggc gggggcggca atcgacgtat
    24541 tcccgacgga accggcgacc aatagcgatc catttacctc tccactgtgt gaattcgaca
    24601 acgtccttct gacgccacac attggcggtt cgactcagga agcgcaggag aatatcggcc
    24661 tggaagttgc gggtaaattg atcaagtatt ctgacaatgg ctcaacgctc tctgcggtga
    24721 acttcccgga agtctcgctg ccactgcatg gtggtcgtcg tctgatgcac atccacgaaa
    24781 accgtccggg cgtgctaact gcgctgaaca aaatcttcgc cgagcagggc gtcaacatcg
    24841 ccgcgcaata tctacaaact tccgcccaga tgggttatgt agttattgat attgaagctg
    24901 acgaagacgt tgccgaaaaa gcgctgcagg caatgaaagc tattccgggt accattcgcg
    24961 cccgtctgct gtactaattc cccttctctg aaaattaacg ggcaggtcac tgacttgccc
    25021 gtttttttat cccttctcca caccgttgcc gcgttgcgca ttctactttg cgagtctgct
    25081 ccagaaaata aatcaaagtc agatcaatgc gttgtgttta tttaatgggg atttacgatg
    25141 gcgggacagc atggggctgt aacggtaaag ccctcaccga agcgagggct taaaggagaa
    25201 gggttatgat gcgacttgtc atcatactga ttgtactgtt actgataagt ttcggagctt
    25261 attaacagtc agtctcaggg gaggagtcat cctcccttac ccttactcac taaattaggt
    25321 caaagaatca acgatgtcaa tcagggcgat gcggggtgca tcgccctgaa ccgaactttt
    25381 tgatagccac ttcatcgccc acgtttactg ccgtaataga tcctttttcc caaaaattat
    25441 ctctgttttg catggggttt ttcgagcacg ctcgaagaaa agatttaaag gtagatcaat
    25501 gcgttgtggt tatttaaaga gagcttacga tagctgggca gcatggggct gtaacggtaa
    25561 agccctcacc gaagcgaggg cttgaaggag aagggttatg atgcgacttg ccatcatact
    25621 gattgtactg ttactcataa gtttcccagc ttattaacag tcagtcacag gggaggagca
    25681 atcctccctt acccttactc actaaattag gttaaagaat caacgatgtc aatcagggcg
    25741 atgcaggtgt atcgccctta ccactcccag actttcgacg gtgtaaccac cgcaggaaga
    25801 gggatatccc actcttcaac ggggagtttt tccaccaact gacaatcatg cgcataaccc
    25861 accggttgcg ttttatagtg ctgccagttt tgtaaggtcc gatcataaaa accaccgccc
    25921 attcccaggc gctgaccgta ctcatcaaag gcgaccagcg gtgtgatcag cacgtctaat
    25981 cgggaaaggg gtagcacgtc acgcacatcc agttttggct catggatctt caacctgttc
    26041 atcaccagtt cgctttgcgg atggtaattc aggaacagca aattaccggc actaaaggga
    26101 tgcaaaactg gaagatatac gcgcttaccg gcgcgccaga gttgttctat tagcggctgg
    26161 gtgtcgagtt caccatcaaa agagaggaat acagcgaccg tatgcgccat caccaccggg
    26221 ggataagtca tcatccgggt agcggcttgt tgacccattt ccagctgttg ttccggcgtt
    26281 aacgcacgac gacgttgccg aatcattttg cggatttctt gtcgggataa tgtcagtggg
    26341 agctctggta gttgcgtcat ggctggtgcc agataagaag ggaatctccg agatgccgcc
    26401 gcaggctgta acccttgaac ccttggttca aggtgaatgt gtcgtcgcag ttttaaggct
    26461 tctcggacga accgagcatg ctcaccaacc gcggagcgcc acattcttgt ggtatgaaat
    26521 atcggctcag gggactggcc cgcttgcgaa catctcagag aaattttgtc ttcacggtta
    26581 ctctaccaca gtaaaccgaa aagtgtcatt caaagttttg gttagttttt tcggtgatgc
    26641 gaccttgttc aagtaacgct tgttctatgg tctgctgcag catccgaata cgctgttcca
    26701 tacttgccgc gtagtcacga gtctttgctt tttcttgcgc taactcatag ctgatattca
    26761 atgcggcaat gaagaccaac tgttcagtat ttgtgactct agtgcgttct ttcagatctt
    26821 gcaaccgttg gttcagatcg tccgctgcct gattcaacgc atccctttgg tcaggcgggc
    26881 agttcacacg cagtgaacgg ccaaaaattt ggatatcgac gggttgtgca gacatgccac
    26941 cttcctgctg attgactgcg ctgccttcgt ctttagaccc atggtctgcg aaggggcgac
    27001 actatagcta ccctgatgag aagagacaag cccttttctg gtccaccagg gcccaaagtg
    27061 gtagcatatc atgaatattc ctccctttga cgacgaatgc ttatgtctat acagaacgaa
    27121 atgcctggtt acaacgaaat gaaccagtat ctgaaccaac aagggacggg tctgacccct
    27181 gccgagatgc atggtttaat cagtgggatg atatgtggcg gtaacgatga cagctcatgg
    27241 ctgccgctac ttcacgacct gacgaacgaa ggcatggctt tcggtcatga gctggcacag
    27301 gcactgcgta aaatgcactc tgccaccagc gatgccctgc aggatgacgg cttccttttt
    27361 cagctttatc tgcctgatgg cgatgatgtc agcgttttcg atcgggctga tgcactggca
    27421 ggttgggtca atcacttcct gcttggtctt ggcgttacgc aaccgaagct ggacaaagtg
    27481 accggcgaaa ccggtgaagc catcgacgat ctgcgtaaca tcgcgcagct gggctacgac
    27541 gaagacgaag atcaggaaga gcttgaaatg tcgcttgaag agatcatcga atacgttcgt
    27601 gttgccgcgc tgttatgcca cgacaccttt actcatccgc aaccgaccgc gccagaagta
    27661 caaaaaccga ctctacacta aaaacaaaaa acgtaaggag agtgttatga gtgagatatc
    27721 ccggcaagag tttcagcgtc gccgtcaggc cctggtggag caaatgcaac ccggcagcgc
    27781 cgcgctgatt tttgctgcac cagaagtaac acgtagcgcc gacagcgaat acccctatcg
    27841 tcagaacagt gacttctggt acttcaccgg ctttaacgaa ccggaagcgg tgctggtgct
    27901 gattaaaagc gatgacactc ataaccacag cgttctgttt aaccgcgttc gcgacctgac
    27961 ggcggagatc tggtttggcc gtcgcttagg ccaggatgcc gcgccagaga aactgggcgt
    28021 tgaccgcgca ctggcattca gcgaaatcaa tcagcaactt tatcaactac ttaacggcct
    28081 ggatgtggtt taccatgccc aggacgaata tgcatatgct gatgaaatcg tgaacagtgc
    28141 gctggaaaaa ctgcgtaaag gttcgcggca aaatctcacc gcaccggcaa cgatgatcga
    28201 ctggcgtcct gttgttcatg aaatgcgcct gttcaaatcg ccagaagaga ttgccgtact
    28261 ccgccgcgcg ggagaaatca ccgccatggc acatacacgg gcgatggaaa aatgccgtcc
    28321 gggaatgttc gagtaccatc tggaaggcga aattcaccac gaatttaacc gccacggtgc
    28381 gcgctatcct tcctataaca ccattgtagg cagcggtgaa aacggctgca ttctgcacta
    28441 caccgaaaac gagagtgaac tgcgcgacgg tgacctggtg ttgattgacg cgggttgtga
    28501 atacaaaggt tacgcgggcg atattacccg caccttcccg gtcaacggca aattcaccca
    28561 ggcccagcgt gaaatctacg acattgtgct ggagtctctc gaaaccagcc tgcgcctgta
    28621 tcgtccggga acttccattc tggaagtcac tggtgaagtg gtgcgcatca tggttagcgg
    28681 cctggtaaaa ctcggcatcc tgaaaggtaa tgttgatgaa ctgatcgctc agaacgccca
    28741 tcgtcctttc tttatgcatg gccttagcca ctggttagga ctggatgtcc atgacgttgg
    28801 cgtttatggt caggatcgct cgcgcattct ggaaccgggg atggtactga ccgtagagcc
    28861 tggtctgtat atcgcgccgg atgcggatgt gccagaacaa tatcgcggta tcggcattcg
    28921 tattgaagac gacattgtga ttaccgaaac cggcaacgaa aacctcaccg ccagcgtggt
    28981 gaaaaagccg gaagaaatcg aagcgttgat ggctgctgcg agaaagcaat gagcgtaatc
    29041 atcgtcggtg gcggcatggc gggcgcgacg ctggcgctgg ctatttcccg gttaagtcac
    29101 ggggcgctgc cggtacattt gattgaagcg actgcgccag agtcacatgc tcatccgggc
    29161 tttgatggac gagcgatagc gctggcggcg ggtacctgtc agcaactggc gcgcatcggc
    29221 gtctggcaat ctctggctga ttgcgcaacc gccatcacta ccgtgcatgt cagcgatcgt
    29281 ggtcatgctg gattcgtcac cctcgtcgca gaagattacc aactggcggc gctggggcag
    29341 gttgtcgaat tgcataatgt cgggcaacgg ctgtttgcat tgctgcgaaa agcacctggc
    29401 gtaatgctgc attgccccga tcgcgtagct aacgttgccc gtactcagag tcacgttgaa
    29461 gtgacgctgg agagcggcga gatgctgacg ggccgcgtgc tgatagccgc tgatggcacc
    29521 cattcagcgt tagccaccgc gtgcggcgtt gattggcagc aggagcctta cgaacaactg
    29581 gcagtgattg ccaacgttgc tacttctgtt gcgcacgaag gccgcgcttt tgaacgcttc
    29641 acgcaacatg gcccgctggc gatgttgccg atgtctgacg gacgctgttc gctggtctgg
    29701 tgtcatccac tggaacggcg cgaagaggtg ctgtcgtgga gcgacgagaa gttttgccgt
    29761 gaactccagt ctgcctttgg ctggcgactg gggcaaatta cccacgccgg taaacgcagt
    29821 gcttatccgc tggcattaac ccgcgccgcc agaccgatta cccatcgcac tgtgctggtg
    29881 ggcaatgcgg cgcaaactct gcacccgatt gccgggcaag ggtttaacct cggtatgcga
    29941 gatgtcatga gccttgcgga aaccctgact caggcgcatg agcgcggaga agacatcggt
    30001 gattacggca tattgtgccg ctatcagcag cgtcgacaga gcgatcgcga agcaaccatc
    30061 ggcgtcacgg acagcctggt acatcttttt gccaaccgtt gggcaccgct ggttgtcggg
    30121 cgcaacatcg gactgatgac gatggaatta ttcaccccgg cacgcgatgt gctggcgcag
    30181 cgcaccctcg gttgggtggc gcgttgaagg cggttttaag gaataaacaa tgcaaagtgt
    30241 tgatgtagcc attgttggcg gcggcatggt ggggctggcg gttgcctgcg gcatacaggg
    30301 gagcggctta cgcgttgccg tactggagca gcgcgtaccg gaacctctgg cggtagatgc
    30361 actaccacaa ctgcgcgttt cggctatcaa tgccgccagc gaaaaattac tcacccgtct
    30421 tggcgtctgg caggacattc tcgctcgtag ggctagttgc tatcacggca tggaagtgtg
    30481 ggacaaagac agttttggtc acatctcgtt tgacgatcaa agcatgggct atagccatct
    30541 tgggcacatc gttgaaaact cagtgattca ctacgcgctg tggaacaaag cgcagcagtc
    30601 gtcagatatt actctgttgg cccccgcaga attacagcag gtcgcctggg gagaaaatga
    30661 aaccttcctg acgctgaaag atggcagcat gttaacggcg cgtctggtga ttggcgcgga
    30721 cggtgctaat tcctggttgc gcaacaaagc agatattccg ctgactttct gggattatca
    30781 gcatcacgcg ctggtagcga ccattcgcac ggaagaaccg catgatgcgg tggcgcggca
    30841 ggttttccat ggcgaaggca ttctggcctt tttaccgctt agcgatccgc atctttgctc
    30901 gattgtctgg tcactgtcgc cagaggaagc gcagcggatg cagcaggcaa gtgaagacga
    30961 atttaatcgc gcgttaaata tcgcttttga taatcgcctt ggcttatgca aggttgagag
    31021 cgcgcgtcag gtgttcccgc tgacggggcg ttatgcgcgc cagtttgccg cacaccgtct
    31081 ggcgctggtg ggcgacgccg cgcataccat tcacccactg gcggggcagg gcgttaatct
    31141 cggatttatg gacgccgcag agctgattgc tgaactgaaa cggttgcatc gtcagggtaa
    31201 agacatcggg cagtacattt acctgcgccg ctatgaacgt agccgcaagc acagcgcggc
    31261 gctgatgctg gctggtatgc agggttttcg cgatctcttt tccggcgcta atccggcgaa
    31321 aaaactgctg cgtgatattg gtttgaaact ggccgacacg cttcctggcg ttaagccgca
    31381 acttatccgc caggcaatgg gattaaacga tttgcctgaa tggctgcgtt aaaaatttct
    31441 cctctgttgt ttatttgata cccatcacac tttcatctcc cggttttttc gccgggagat
    31501 tttcctcatt tgaaataaac taatttcacc tccgttttcg cattatattt tctaatgcca
    31561 tgattttttg atttagtgtt ttttgacatt tttttagctc ttaatattgt cttatccaaa
    31621 ttgactttct catcacatca tctttgtata gaaattggtg tattttttgg ttttttattc
    31681 tgtcgcgatt tttgcatttt ttaaccataa gctaatgtga tgatcaattt taccttatgg
    31741 ttaacagtct gtttcggtgg taagttcagg caaaagagaa cgattgcgtt ggggaccggg
    31801 agtggctccg atgctgggtt ttgtggtgat aatttcacca tgaaaaagtt gtcagccccg
    31861 cttattcaat gaggacaaga tggcacaaca gactcctttg tacgaacaac acacgctttg
    31921 cggcgctcgc atggtggatt tccacggctg gatgatgccg ctgcattacg gttcgcaaat
    31981 cgacgaacat catgcggtac gtaccgatgc cggaatgttt gatgtgtcgc atatgaccat
    32041 cgtcgatctc cgcggcagcc gcacccggga gtttctgcgt tatctgctgg cgaacgatgt
    32101 ggcgaagctc accaaaagcg gcaaagccct ttactcgggg atgttgaatg cctctggcgg
    32161 tgtgatagat gatctcatcg tctactactt tactgaagat ttctttcgcc tcgttgttaa
    32221 ctccgccacc cgcgaaaaag acctctcctg gattacccaa cacgctgaac ctttcggcat
    32281 cgaaattacc gttcgtgatg acctttccat gattgccgtg caagggccga atgctcaggc
    32341 aaaagctgcc acactgttta atgatgctca gcgtcaggcg gtggaaggga tgaaaccgtt
    32401 ctttggcgtg caggtgggcg atctgtttat tgccaccact ggttataccg gtgaagcggg
    32461 ctatgaaatt gcgctgccca atgaaaaagc ggccgatttc tggcgtgcgc tggtggaagc
    32521 gggcgttaag ccctgcggtc tgggggcgcg tgacacgctg cgtctggaag cgggcatgaa
    32581 tctttatggt caggagatgg acgaaactat ttctccttta gccgccaaca tgggctggac
    32641 tatcgcctgg gaaccgacag atcgtgactt tatcggtcgt gaagccctgg aagtgcagcg
    32701 tgagcatggt acagaaaaac tggttggtct ggtgatgacc gaaaaaggcg tgctgcgtaa
    32761 tgaactgccg gtacgcttta ccgatgcgca gggcaaccag catgaaggca ttatcaccag
    32821 cggtactttc tccccgacgc tgggttacag cattgcgctg gcgcgcgtgc cggaaggtat
    32881 tggcgaaacg gcgattgtgc aaattcgcaa ccgtgaaatg ccggttaaag tgacaaaacc
    32941 tgtttttgtg cgtaacggca aagccgtcgc gtgatttact tttttggaga ttgattgatg
    33001 agcaacgtac cagcagaact gaaatacagc aaagaacacg aatggctgcg taaagaagcc
    33061 gacggcactt acaccgttgg tattaccgaa catgctcagg agctgttagg cgatatggtg
    33121 tttgttgacc tgccggaagt gggcgcaacg gttagcgcgg gcgatgactg cgcggttgcc
    33181 gaatcggtaa aagcggcgtc agacatttat gcgccagtaa gcggtgaaat cgtggcggta
    33241 aacgacgcac tgagcgattc cccggaactg gtgaacagcg aaccgtatgc aggcggctgg
    33301 atctttaaaa tcaaagccag cgatgaaagc gaactggaat cactgctgga tgcgaccgca
    33361 tacgaagcat tgttagaaga tgagtaacgg ctttattcct cttctgcggg agaggatcag
    33421 ggtgaggaaa atttatgcct caccctcact ctcttcgtaa ggagagaggt tcacaattca
    33481 ctgcacgttt caggaaccat cgctcatgac acagacgtta agccagcttg aaaacagcgg
    33541 cgcttttatt gaacgccata tcggaccgga cgccgcgcaa cagcaagaaa tgctgaatgc
    33601 cgttggtgca caatcgttaa acgcgctgac cggccagatt gtgccgaaag atattcagct
    33661 tgcgacacca ccgcaggttg gcgcaccggc gaccgaatac gccgcactgg cagaactcaa
    33721 ggctattgcc agtcgcaata aacgcttcac gtcttacatc ggcatgggtt acaccgccgt
    33781 gcagctaccg ccggttatcc tgcgtaacat gctggaaaat ccgggctggt ataccgcgta
    33841 cactccgtat caacctgaag tctcccaggg ccgccttgaa gcactgctca acttccagca
    33901 ggtaacgctg gatttgactg gactggatat ggcctctgct tctcttctgg acgaggccac
    33961 cgctgccgcc gaagcaatgg cgatggcgaa acgcgtcagc aaactgaaaa atgccaaccg
    34021 cttcttcgtg gcttccaatg tgcatccgca aacgctggat gtggtccgta ctcgtgccga
    34081 aacctttggt tttgaagtga ttgtcgatga cgcgcaaaaa gtgctcgacc atcaggacgt
    34141 cttcggcgtg ctgttacagc aggtaggcac taccggtgaa attcacgact acactgcgct
    34201 tattagcgaa ctgaaatcac gcaaaattgt ggtcagcgtt gccgccgata ttatggcgct
    34261 ggtgctgtta actgcgccgg gtaaacaggg cgcggatatt gtttttggtt cggcgcaacg
    34321 cttcggcgtg ccgatgggct acggtggccc acacgcggca ttctttgcgg cgaaagatga
    34381 atacaaacgc tcaatgccgg gccgtattat cggtgtatcg aaagatgcag ctggcaatac
    34441 cgcgctgcgc atggcgatgc agactcgcga gcaacatatt cgtcgtgaga aagcgaactc
    34501 caacatttgt acttcccagg tactgctggc aaacatcgcc agcctgtatg ccgtttatca
    34561 cggcccggtt ggcctgaaac gtatcgctaa ccgcattcac cgtctgaccg atattctggc
    34621 ggcgggcctg caacaaaaag gtctgaaact gcgccatgcg cactatttcg acaccttgtg
    34681 tgtggaagtg gccgacaaag cgggcgtact gacgcgtgcc gaagcggctg aaatcaacct
    34741 gcgtagcgat atcctgaacg ccgtggggat cacccttgat gaaaccacca cgcgtgaaaa
    34801 cgtaatgcag cttttcagtg tgctgctggg cgatagccac gggctggaga tcgacacgct
    34861 ggacaaagac gtggctcacg acagccgctc tatccagcct gcgatgctgc gcgacgacga
    34921 aatcctcacc catccggtgt ttaatcgcta tcacagcgaa accgaaatga tgcgctatat
    34981 gcactcgctg gagcgtaaag atctggcgct gaatcaggcg atgatcccgc tgggttcctg
    35041 caccatgaaa ctgaacgccg ccgccgagat gatcccaatc acctggccgg aatttgccga
    35101 actgcacccg ttttgcccgc cggaacaggc tgaaggttat cagcagatga ttgcgcagct
    35161 ggctgactgg ctggtgaaac tgaccggtta cgacgccgtt tgtatgcagc cgaactccgg
    35221 cgcacagggc gaatacgcgg gcctgctggc gattcgtcat tatcatgaaa gccgcaacga
    35281 agggcatcgc gatatctgcc tgatcccggc ttctgcgcac ggaactaacc ccgcttctgc
    35341 acatatggca ggaatgcagg tggtggttgt ggcgtgtgat aaaaacggca acatcgatct
    35401 gactgatctg cgcgcgaaag cggaacaggc gggcgataac ctctcctgta tcatggtgac
    35461 ttatccttct acccacggcg tgtatgaaga aacgatccgt gaagtgtgtg aagtcgtgca
    35521 tcagttcggc ggtcaggttt accttgatgg cgcgaacatg aacgcccagg ttggcatcac
    35581 ctcgccgggc tttattggcg cggacgtttc gcacctcaac ctgcataaaa ctttctgcat
    35641 tccgcacggc ggtggtggtc cgggtatggg accgatcggt gtgaaagcgc atctggcacc
    35701 gtttgtaccg ggtcatagcg tggtgcaaat cgaaggcatg ttaacccgtc agggcgcagt
    35761 ttctgcggca ccgttcggta gcgcctctat cctgccaatc agctggatgt acatccgcat
    35821 gatgggcgca gaagggctga aaaaagcaag ccaggtggca atcctcaacg ccaactatat
    35881 tgccagccgc ctgcaggatg ccttcccggt gctgtatacc ggtcgcgacg gtcgcgtggc
    35941 gcacgaatgt attctcgata ttcgcccgct gaaagaagaa accggcatca gcgagctgga
    36001 tattgccaag cgcctgatcg actacggttt ccacgcgccg acgatgtcgt tcccggtggc
    36061 gggtacgctg atggttgaac cgactgaatc tgaaagcaaa gtggaactgg atcgctttat
    36121 cgacgcgatg ctggctatcc gcgcagaaat tgaccaggtg aaagccggtg tctggccgct
    36181 ggaagataac ccgctggtga acgcgccgca cattcagagc gaactggtcg ccgagtgggc
    36241 gcatccgtac agccgtgaag ttgcggtatt cccggcaggc gtggcagaca aatactggcc
    36301 gacggtgaaa cgtctggatg atgtttacgg cgaccgtaac ctgttctgct cctgcgtacc
    36361 gattagcgaa taccagtaat tcactgattc gagtattttc taaaggcgct tcggcgcctt
    36421 tttagtcaga tgacaaagta caaaagtgct cagacagtcc cctcgcccct ttggggagag
    36481 ggttagggtg aggggaacag gccggcactg gcgcgaatat ttaccctcac cccgaccctc
    36541 tccctgaaag ggcgaggggg aaaagcgtgc caacattgaa gattgagcca gtttgttagc
    36601 aatctcaaag atacgtcaac gaattaattt ttctcggaaa aacaaatggc tatagcactt
    36661 gtgaccggtg gcagttgcgg catcgggcgg gcaactgcat tactgttggc gcaagaaggg
    36721 tatacggtgg cggttaatta tcagcaaaac ctccacgcgg cgcaggaagt gatgaactta
    36781 ataatgcaag ccggtggcaa agcattcgtg ctccaggcgg atatcagcga cgaaaaccag
    36841 gtcgttgcga tgtttacagc aatcgatcag cacgatgaac cgctagcagc gctggtcaat
    36901 aacgccggga tcttgtttac ccagtgcacc gttgaaaacc ttaccgcaga gcgaatcaac
    36961 cgagtacttt ccaccaacgt gacgggatat tttctctgct gccgcgaggc ggtaaaacgc
    37021 atggcgctta aaaatggtgg cagtggcggc gctatcgtca atgtctcttc ggtggcctca
    37081 cgattgggtt cgccagggga atatgttgat tacgcggcat cgaaaggggc gattgatacg
    37141 ttaaccaccg gactatcgct ggaagtcgcc gcgcagggga tccgcgttaa ctgcgtgcgg
    37201 ccagggttta tttataccga aatgcacgcc agcggcggcg agcctggacg cgtcgatcgc
    37261 gttaagtcga acatccccat gcagcgtggt ggacaggcag aagaggtcgc gcaggccatt
    37321 gtctggctac taagtgataa agcctcttac gtcacgggaa gttttatcga tttggcgggc
    37381 gggaaataaa atagggaagt tgtctgaccg gatgcaacaa gtattgcatc cggtacttca
    37441 tcgacttaaa gcttctcgcc gttgctggca atcacctctt tgtaccagtt aaagctcttc
    37501 ttacgtgaac gcgacatatc gccagtaccg tcgtcatgtt tattcacata gataaagccg
    37561 tagcgtttgc tgtactgccc ggtagtgaat gacacgcagt cgatgcagcc ccacggtgtg
    37621 tagcccatca gatccacacc atcgtaagtc actgcttttt tcatctcttc aatatgggcg
    37681 cgcaggtagt caatgcggta gtcgtcgttg atgctgccat cttcttccac tttgtcgtaa
    37741 gcgccaaaac cgttttcgac aataaacagc ggcctctgat aacgctcata cagttcgcaa
    37801 agggcatagc gcagacctac tggatcaatc tgccagcccc agtcagacgc tttaacatac
    37861 gggttcggta cgctgccttc aaaaccagag atcgcatcgc cggtgccgcc ttcggccttc
    37921 actgcgttgg tcatgtaata gctgaaacca agataatcgc aggtgccttc gcgcagtaca
    37981 tcgagatcgc cgtcttccat tttgatatta aatccgcgac gttcccactc gttcaataca
    38041 taggacgggt aatagccgca tagctgcaca tcggtaaaga cgtagcgttc gcgcatcgac
    38101 tcctgagcga acatcacatc gtccgggtta catgagtagg gatagagcgg caccatcgcc
    38161 agcatacagc cgactttcat ctccgggtta atgcgacgcg cggctttcac tgccagggcg
    38221 ctggcgacaa actggtgatg cagcacctgg tacatcgtct cttccgggtt gtcatgctcg
    38281 gtatacacca cgccggagca gcagtaacca aacagcggcg cacgccagtt acgctggttg
    38341 tttatttcgt tgaaggtcat ccagtatttg actttatgct tgtagcgttc gaataccact
    38401 tccgcgaaac ggacaaagaa atcaacgact ttacggttgg tccagctacc gtattgctgc
    38461 accagatgca gcggcatttc aaagtgggag agggttatca ccggttcgat gttgtatttc
    38521 agcagttcat cgaacagaga gtcgtaaaac ttcagccctt cttcgtttgg ctgagcttca
    38581 tcaccttttg gaaaaatacg cgtccaagcg atggaagtcc gaaaacattt gaagcccatt
    38641 tcggcaaata gcttgatgtc ttccttgtag tgaccataaa aatcaacggc ttcatggttt
    38701 ggatagtatt ttcctggcaa gacttctttg gtgatttcgc gcggcacgcc gtgtgcgcca
    38761 ccggtcagaa cgtcacaaat gctcggtcct ttgccgcctt tgttccagcc gccttcgacc
    38821 tgatgagcgg caacggcgcc accccataag aaatctttcg gtaaggtgag ttttttcact
    38881 atcataggct ccagatcagt caattcttac aggaaatagt accatgcagc caaacgacat
    38941 cacttttttt caacgtttcc aggatgacat tctggctggg cgtaaaacca tcaccatccg
    39001 cgacgagtct gaatcgcact tcaaaacggg tgatgtgctt cgtgtcggac gttttgaaga
    39061 tgacggttat ttttgcacga ttgaagtcac cgcaacctca accgtaacgc tggatacact
    39121 gacggaaaaa catgcagagc aggaaaatat gaccctgcct gaactgaaaa aggtcattgc
    39181 tgacatctat cccggtcaga cacaatttta tatgattgaa tttaaatgtc tttaaattga
    39241 actcacgcct gttagttgaa atttcgcatt taatttcatg atttttagga gattagtctc
    39301 aaatcagaat ttattttagc taacaggtgt tcactggaac tattctcagt tacgctggag
    39361 aggtatacat gtgcgtgtac gtttctctgg agtaagttat ggttcagaag cccctcatta
    39421 agcagggata ttcgctggca gaggaaattg ccaacagcgt cagtcacggc attgggctgg
    39481 tgtttggtat cgttgggctg gtgttgctac tggttcaggc ggtggatctc aatgccagcg
    39541 ccacagcgat aaccagttac agcctctatg gcggcagtat gatcctgctg ttcctcgctt
    39601 cgacgctcta tcacgccatt cctcatcaac gggcaaaaat gtggctgaag aaatttgacc
    39661 attgcgctat ttacctgttg attgccggaa cctacacgcc gtttttgctg gtggggctgg
    39721 attctccgtt agcgcgcggg ttgatgattg ttatctggag cctggcattg ctggggattc
    39781 tgttcaaact gaccatcgcg caccgattca aaattttatc tctggtgact tatctggcga
    39841 tgggctggct gtcgctggtg gtaatttatg aaatggcagt taagctcgcg gcgggcagcg
    39901 ttaccttact ggcggtaggc ggcgtggttt attcgctcgg ggtgattttc tacgtctgca
    39961 aacgcattcc atacaaccat gccatctggc acggcttcgt gctcggcggt agtgtgtgcc
    40021 actttctggc gatctatttg tatattgggc aggcgtaatg ggtatgcctt tgtaggcctg
    40081 ataagacgcg tcagcgtcgc atcaggcatc ggagcgcaac cgccggatgc ggcgtgaacg
    40141 ccttatccga tctacgggcc tcgtccctgt tgtgataaag gcatcaaatc ccagacatga
    40201 taagacgcgt cagcgtcgca tcaggcgata aatgcctgat gcgatacaga gatttactct
    40261 tcgagcgaat acggcagcgg ctcgatatgc agtgtattcg catcgtcgcg tacgcggaag
    40321 atgctgtccg gttccatatc gttattcatg acgacctgta ccacgacctg accatcttcc
    40381 agttttaccg cagccagcgc cgtaccggta cgacgccagt tctcgcccat tttcagctct
    40441 aagtcttcac cagcttccgg cagtcggctg gcactacctg ccagcaacca gagcgcgcgt
    40501 ttgttggcac cacggaattt tgctcgcgcc accatctctt gtccggtata acagcctttc
    40561 ttaaagctga taccgcccag cgcctggaga ttggtcgcct gtgggataaa ctgcccgctg
    40621 ttggcggcat caatcaccgg gaaaccagct tcaatgttta atgccagcca ctgttggcta
    40681 ttgttcagtt ccgcttcacc gcgcagttta tcggtcagca tattagcagt agcttcatcg
    40741 gttacgatca ggaaacgttc tgccgggtgt tcaaaccata gcaaagtggt cgcgccttct
    40801 ttgactacct gtttttcttt cgaaggcagt tcgctaaaga gatttgccag cgcggcgcgc
    40861 gcctgaaaac cggcaacacc aagcagcaca cgctcgtcgt ctggagcgat ggtcacttta
    40921 gagaataccg catatttttt cagttcagtc agctgcggtt cacgcacgct gcgccgttca
    40981 atccatgcaa agccatcgcc gtcgcggaac agacgtaaat tgctccacat tttacctttg
    41041 gcgtcgcaat gggcggcgag caggtgctga tcttctgcca tctggctgac atctgctgtc
    41101 acctgaccct gcatatattt ttcgctgtcc gcgccagtaa tggtggcaag cgcccagtca
    41161 tcaagcgtca tcagcgtcag cggtaaacgg gcagaagccg taggctgacg tggaggaaaa
    41221 ggtgtaaaag ccataataga gtcctgagtt gcttaacggc gtattaatga ctaatggtaa
    41281 aagagctatg aggcaatgca agtgctttca ctacgcgatc tgtgccttct gcgaggcgac
    41341 tgccaaaaaa atgaagccct tgaattgttg tgaattaatt tcgcgatcgc gatggtgttt
    41401 actgatattc ccgtaaaaga agtcgaaaaa cacgttacaa tagccgggta ctggattttc
    41461 gtgagacagg aagaagatgg acattaacaa caaagcccgc attcattggg catgccgccg
    41521 tggtatgcgc gaactcgata tttcaatcat gccgtttttc gaacatgagt acgacagctt
    41581 aagcgatgac gaaaaacgca tctttattcg tctgctggaa tgtgacgatc cggacctgtt
    41641 taactggctg atgaatcacg gtaaaccggc cgatgcagaa ctggaaataa tggtccgact
    41701 catccagaca cggaaccggg aacgtggtcc tgtggcaatc tgatttgcgc gtctcctggc
    41761 gcgcacagtg gctttccttg ctgattcatg ggctggttgc cgctgttatt ttactcatgc
    41821 cctggccgct cagttacacc ccgttatgga tggtgttact ttcgctggtg gtatttgatt
    41881 gcgttcgcag ccagcggcgt attaatgctc gccaggggga aattcgcttg ttgatggacg
    41941 ggcgtttgcg ttggcaaggg caggagtgga gcatcgtcaa agcaccgtgg atgattaaga
    42001 gctgcatgat gctgcgttta cgttctgatg gcggtaaacg gcaacattta tggctggcag
    42061 ccgacagcat ggacgaagcc gaatggcggg atttacggcg gattttgttg caacaagaga
    42121 cgcaaagata aagatgacgg caaagcatat gcgctaagcc gtcagtgaga tcaggcgtaa
    42181 tgctctgcca tttcgttgag gatttgctcg caccagctct gaatacgctc gtcgctaagg
    42241 tcatactggt tagtttcatc cagcgccaga cccacgaaca gttgcccgtc agcaatcacc
    42301 ggtttcgggc tggtaaattc atatccttcc gttggccagt agccgacgaa cttcacgcct
    42361 ttggtcgaga gtttgtcatg cagcataccg agcgcatcga ggaaccactc gccgtatccc
    42421 agttgatcgc caagcccata cagcgcaaca attttacctt caaggttcag gtcgtcgagc
    42481 tgatcccaga cggcttccca gtcttcctgg atttcaccaa aatcccaggt cgggataccc
    42541 agaatgagca catcgtactg ctccattaat ttcggggagt cgtccttgag gttatgtaag
    42601 gtcaccagtt ctgggccgat aatatcgcgg attttttctg ccgccatttc ggtgtaacag
    42661 gtgctggaac cgtaaaaaag acccatattc ataacgtaag cttctcgatt ctgctgtggc
    42721 ttttgcagct agtgtatcag aatcgcttta gtcacggcac aataaggcat aatggatcaa
    42781 atgagtgacc ataaggggcg caagtgaaac aggatctggc acgcatcgag cagtttcttg
    42841 atgctctgtg gctggaaaaa aatctggctg aaaatacgtt gaacgcttac cgtcgcgatc
    42901 tgtcaatgat ggtggagtgg ttgcatcacc gcgggttgac gctggcgacg gcgcaaagtg
    42961 acgatttgca ggcattactg gcagaacggc tggagggcgg gtataaagcc accagctcag
    43021 cgcgtttgct gagtgcagtg cgccgattgt tccagtatct ttatcgcgaa aagtttcgtg
    43081 aagacgatcc cagtgcgcat ctcgcttcac cgaaattgcc ccagcgtttg ccaaaagatt
    43141 taagcgaagc gcaggtcgaa cgtttattac aggcgccatt aattgatctg ccactggagc
    43201 tacgcgataa agccatgctt gaagtgttgt atgctaccgg gctgcgtgtc tctgaactgg
    43261 tcgggctgac aatgagtgat atcagcctgc gtcagggcgt agtgcgggtc attggtaaag
    43321 gcaacaaaga gcgcctggtg ccgttagggg aagaggcggt ttactggctg gaaacctatc
    43381 tggaacatgg gcgtccgtgg ctgctgaatg gtgtgtcaat tgacgtgttg tttcccagcc
    43441 agcgtgcgca gcagatgacg cgacagacct tctggcaccg cattaaacat tatgccgtgc
    43501 tggcgggtat cgacagcgaa aagctgtcac cgcatgtgtt gcgtcacgct tttgccactc
    43561 atttattaaa tcatggtgcg gatttacgcg tggtgcagat gctactgggc cacagcgatc
    43621 tttccaccac gcaaatttat acgcatgtcg ctaccgagcg tctgcggcaa cttcatcaac
    43681 agcatcaccc gcgggcgtga tgtctgaaaa gaacgggaag atttatgaag aaaggtttta
    43741 tgttgtttac tttgttagcg gcgttttcag gctttgctca ggctgatgac gcggcaattc
    43801 aacaaacgtt agccaaaatg ggcatcaaaa gcagcgatat tcagcccgcg cccgtagctg
    43861 gcatgaagac agttctgact aacagcggtg tgttgtacat caccgatgat ggtaaacata
    43921 tcattcaggg gccaatgtat gacgttagtg gcacggctcc ggtcaatgtc accaataaga
    43981 tgctgttaaa gcagttgaat gcgcttgaaa aagagatgat cgtttataaa gcgccgcagg
    44041 aaaaacacgt catcaccgtg tttactgata ttacctgtgg ttactgccac aaactgcatg
    44101 agcaaatggc agactataac gcgctgggga tcaccgtgcg ttatcttgct ttcccgcgcc
    44161 aggggctgga cagcgatgca gagaaagaaa tgaaagctat ctggtgtgcg aaagataaaa
    44221 acaaagcgtt tgatgatgtg atggcaggta aaagcgtcgc accagccagc tgcgacgtgg
    44281 atattgccga ccattacgca cttggcgtcc agcttggcgt tagcggtact ccggcagttg
    44341 tgctgagcaa tggcacactt gttccgggtt accagccgcc gaaagacatg aaagaattcc
    44401 tcgacgaaca ccaaaaaatg accagcggta aataattcgc gtgaaacaac agatacaact
    44461 tcgtcgccgt gaagtcgatg aaacggcaga cttgcccgct gaattgcctc ccttgctgcg
    44521 ccgtttatac gccagccggg gagtacgcag tgcgcaagaa ctggaacgca gtgttaaagg
    44581 tatgttgccc tggcagcaac taagcggtgt cgaaaaggcc gttgagatcc tttacaacgc
    44641 ttttcgcgaa ggaacgcgga ttattgtggt cggtgatttc gacgccgacg gcgcgaccag
    44701 cacggctcta agcgtgctgg cgatgcgctc gcttggttgc agcaatatcg actacctggt
    44761 accaaaccgt ttcgaagacg gttacggctt aagcccggaa gtggtcgatc aggcccatgc
    44821 ccgtggcgcg cagttaattg tcacggtgga taacggtatt tcctcccatg cgggagttga
    44881 gcacgctcgc tcgttgggca tcccggttat tgttaccgat caccatttgc cgggcgacac
    44941 attacccgca gcggaagcga tcattaaccc taacttgcgc gactgtaatt tcccgtcgaa
    45001 atcactggca ggcgtgggtg tggcgtttta tctgatgctg gcgctgcgca cctttttgcg
    45061 cgatcagggc tggtttgatg agcgcggcat cgcaattcct aacctggcag aactgctgga
    45121 tctggtagcg ctggggacag tagcggacgt cgtgccgctg gacgctaata atcgcattct
    45181 gacctggcag gggatgagtc gcatccgtgc cggaaagtgc cgtccaggga ttaaagcgct
    45241 gctggaagtg gcaaatcgcg atgcacaaaa actagccgcc agcgatttag gttttgcgct
    45301 gggaccgcgt ctcaatgctg ccgggcgact ggatgatatg tccgttggtg tggcgctgtt
    45361 gctgtgcgac aacatcggcg aagcgcgcgt gctggcaaat gaactcgatg cgctaaacca
    45421 gacgcgaaaa gagatcgaac aaggaatgca ggttgaagcc ctgaccctgt gcgagaaact
    45481 ggagcgcagc cgtgacacgc tacccggcgg gctggcaatg tatcaccccg aatggcatca
    45541 gggcgttgtc ggtattctgg cttcgcgtat caaagagcgt tttcaccgtc cggttatcgc
    45601 ctttgcgcca gcaggtgacg gtacgctgaa aggttccggt cgctccattc aggggctgca
    45661 tatgcgtgat gcgctggagc gattagacac actctacccc ggcatgatgc tcaagtttgg
    45721 cggtcatgcg atggcggcgg gtttgtcgct ggaagaggat aaattcgaac tctttcaaca
    45781 acggtttggc gaactggtta ctgagtggct ggccccttcg ctattgcaag gcgaagtggt
    45841 gtcagacggt ccgttaagcc cggccgaaat gaccatggaa gtggcgcagc tgctgcgcga
    45901 tgctggcccg tgggggcaga tgttcccgga gccgctgttt gacggtcatt tccgtctgct
    45961 gcaacagcgg ctggtgggcg aacgtcattt gaaggtgatg gtcgaaccgg tcggcggcgg
    46021 tccactgctg gatggtattg cttttaatgt cgataccgct ctctggccgg ataacggcgt
    46081 gcgcgaagtg caactggctt ataagctcga tatcaacgag tttcgcggca accgcagcct
    46141 gcaaattatc atcgacaata tctggccaat ttagcgtcat cttctctata aaaaagagcg
    46201 tggattgggt acaatcccgc tcttatcacc gcattttgac tagctcaata aaagaaatca
    46261 gaccatgttt gaaattaatc cggtaaataa tcgcattcag gacctcacgg aacgctccga
    46321 cgttcttagg gggtatcttt gactacgacg ccaagaaaga gcgtctggaa gaagtaaacg
    46381 ccgagctgga acagccggat gtctggaacg aacccgaacg cgcacaggcg ctgggtaaag
    46441 agcgttcctc cctcgaagcc gttgtcgaca ccctcgacca aatgaaacag gggctggaag
    46501 atgtttctgg tctgctggaa ctggctgtag aagctgacga cgaagaaacc tttaacgaag
    46561 ccgttgctga actcgacgcc ctggaagaaa aactggcgca gcttgagttc cgccgtatgt
    46621 tctctggcga atatgacagc gccgactgtt acctcgatat tcaggcgggg tctggcggta
    46681 cggaagcaca ggactgggcg agcatgcttg agcgtatgta tctgcgctgg gcagaatcgc
    46741 gtggtttcaa aactgaaatc atcgaagagt cggaaggtga agtggcgggt attaaatccg
    46801 tgacgatcaa aatctccggc gattacgctt acggctggct gcgtacagaa actggcgttc
    46861 accgcctggt gcgtaaaagc ccgtttgact ccggcggtcg tcgccacacg tcgttcagct
    46921 ctgcgtttgt ttatccggaa gttgatgatg atattgatat cgaaatcaac ccggcggatc
    46981 tgcgcattga cgtttatcgc gcgtccggcg cgggcggtca gcacgttaac cgtaccgaat
    47041 ctgcggtgcg tattacccac atcccgaccg ggatcgtgac ccagtgccag aacgaccgtt
    47101 cccagcacaa gaacaaagat caggccatga agcagatgaa agcgaagctt tatgaactgg
    47161 agatgcagaa gaaaaatgcc gagaaacagg cgatggaaga taacaaatct gacatcggct
    47221 ggggcagcca gattcgttct tatgtccttg atgactcccg cattaaagat ctgcgcaccg
    47281 gggtagaaac ccgcaacacg caggccgtgc tggacggcag cctggatcaa tttatcgaag
    47341 caagtttgaa agcagggtta tgaggaacca acatgtctga acaacacgca cagggcgctg
    47401 acgcggtagt cgatcttaac aatgaactga aaacgcgtcg tgagaagctg gcgaacctgc
    47461 gtgagcaggg aattgccttc ccgaacgatt tccgtcgcga tcatacctct gaccaattgc
    47521 acgcagaatt cgacggtaaa gagaacgaag aactggaagc gctgaacatc gaagtcgccg
    47581 ttgctggccg catgatgacc cgccgtatta tgggtaaagc gtctttcgtt accctgcagg
    47641 atgtgggtgg ccgcattcag ctgtacgttg cccgtgacga tctgccggaa ggcgtttaca
    47701 acgagcagtt caaaaaatgg gacctcggcg acatcctcgg cgcgaaaggt aaactgttca
    47761 aaaccaaaac cggcgaactg tctatccact gtaccgagct gcgtctgctg accaaagcac
    47821 tgcgtccgct gccggataaa ttccacggct tgcaggatca ggaagcgcgc tatcgtcagc
    47881 gttatctgga tctcatctct aacgatgaat cccgtaacac ctttaaagta cgctcgcaga
    47941 tcctctctgg tattcgccag ttcatggtga accgcggctt tatggaagtt gaaacgccga
    48001 tgatgcaggt gatcccaggc ggtgccgctg cgcgtccgtt tatcacccac cataacgcgc
    48061 tggatctcga catgtacctg cgtatcgcgc cggaactgta cctcaagcgt ctggtggtcg
    48121 gtggcttcga gcgtgtattc gaaatcaacc gtaacttccg taacgaaggt atttccgtac
    48181 gtcataaccc agagttcacc atgatggaac tctacatggc ttacgcagat tacaaagatc
    48241 tgatcgagct gaccgaatcg ctgttccgta ctctggcaca ggatattctc ggtaagacgg
    48301 aagtgaccta cggcgacgtg acgctggact tcggtaaacc gttcgaaaaa ctgaccatgc
    48361 gtgaagcgat caagaaatat cgcccggaaa ccgacatggc ggatctggat aacttcgact
    48421 ctgcgaaagc gattgctgaa tctatcggta tccacgttga gaagagctgg ggtctgggcc
    48481 gtatcgttac cgagatcttc gaagaagtgg cagaagcaca tctgattcag ccgaccttca
    48541 ttactgaata tccggcagaa gtttctccgc tggcgcgtcg taacgacatt aacccggaaa
    48601 tcacagaccg ctttgagttc ttcatcggtg gtcgtgaaat cggtaacggc tttagcgagc
    48661 tgaatgacgc ggaagatcag gcgcaacgct tcctggatca ggttgccgcg aaagacgcag
    48721 gtgacgacga agcgatgttc tacgatgaag attacgttac cgcactggaa catggcttac
    48781 cgccgacagc aggtctgggg attggcatcg accgtatggt aatgctgttc accaacagcc
    48841 ataccatccg cgacgttatt ctgttcccgg cgatgcgtcc ggtgaaataa gcattacgtt
    48901 atgctcacaa ccccggcaaa tgtcggggtt ttttatttaa gctgggtaaa tgcagataat
    48961 cgtattctgg cttcgcgatt tgtcgcctgc atcaccatcc acggactgaa cgcccacggc
    49021 gtggcatcaa taccgcgtaa tacatcagct aaatcacacc attgataatc catcacttca
    49081 tcatcgttga tctgtaacgc actggtggtg cgtgcggcaa ataccggaca cacttcattt
    49141 tccacaatgc cactcggatc ggtggcgcgg tagcgaaagt caggatagat agattcagga
    49201 ggcgtaattt ccacgccaag ctcataacgg caacggcgga tcaccgcgtc ttcgctgctt
    49261 tctcccagtt gtgggtgccc acaaaccgag ttagtccaca cgccaggcca cgcttttttg
    49321 ctcagtgcgc ggcgggtaac taataattgc cccttggcat taaacagcca actggagaac
    49381 gcgagatgta agagggtgtc tgccgtgtgt gcggcatact tttccagcgt acccgtggga
    49441 actccctgtg cattcaataa aatgacgtgt tccgtttgca taatttctca catgtaattc
    49501 tgatcactca tcattataga taattgaaga gtgaaatgat taaccaagag agcgtttatc
    49561 gacaaccgcc cgttttgagc ggatgccgac aggtaaaaac catttaaaaa agtagcatca
    49621 tatatttgaa gcgactttca atggccattg caacacagtc agaaatgaca ggaatagaga
    49681 gagtaaaaag caacaacaga ctgaatatag cgcgcattaa aaaattcata ttattctcca
    49741 tgctcatttt tcttcagctt gcgcatcaga agccacatac gtactgtcct gacttttaaa
    49801 tctttatcag agacggattc cttcaccagg tcagcatgat gctgatagcc attaaagaag
    49861 acgttcagca caacggcgct aagggttgcg agcataatgc cgctatgtag cagcggttgc
    49921 agtacggccg gtaacttaga aaagaaatcg tgagagagcg tcggagtcat gccaacgccg
    49981 agactgatcg ccacaatata gaggttgtaa cggttggtgg tgtagttaca gcgcgacaga
    50041 attcgaatcc ctgtcgccag taccatgccg aacatcacta gaccagcgcc gcccagcaca
    50101 aattgcggaa tggaggctac cagcaccgcc atttttggca ccatgccaaa caggattaat
    50161 ataattcccg aagaaataca cacccagcgg ctatgaacgc gcatcacgct aaccaggcca
    50221 acgttttgag aaaatgacgt gtgggggaag ctgttaaacg tgccgcctat cattgtccct
    50281 acgccatcga cacgcagccc gcgaataata tcgtgtgaag agagtttacg accgactatt
    50341 tcacccagtg ccaggaacat ccccatcgac tcgataaaca cgatgattaa cacggcagtc
    50401 atggtcagaa tggaaacggg atcgaaaatc ggcataccaa acgacatcgg ggtgacaatc
    50461 gcaaaccacg aggcatcatg tagcccggat aaattgactt cattcatcat ccacgaaagt
    50521 aaaaagccaa atacaatccc cagtaatacg gcgacgttgg acataaatcc tttcgcatag
    50581 cgagtaatga gcaagataaa aattaagacg gcaaaggaga tacctaaata aacgggatta
    50641 ccatattgcg gattcccttt acctccagcg gcccagtcaa tacccacctg aatgatgcta
    50701 agcccaatag aagtaataac cacaccggta accagtggcg ggaataaagg catcaagcga
    50761 ccaataagtg gcgctaataa tgtggtgata aaacccgcgg cgatagtggc accaaatatc
    50821 cccagcaggc cgatatccgg gttcatccca atggctatca ttggtgttac agcagcaaag
    50881 gtcaccgaca taatcaccgg caggcggatc cccataaagc ggccgatacc gatacattgc
    50941 aataatgtga cgatcccgca gcaaaagaga tccgagctaa tgagcatcgc aatagcatct
    51001 tttgagaggc ccagtcggtc accaatcata agaggaacag cgactgcacc tgcgtacatg
    51061 acaaggacgt gttgcagacc gagtatgatc agctttcctg gtgataatat gcggtcaacc
    51121 tcatcagttg ggcggtcttg cccagaagat gagggaagtt gggaatctat ggcgctcatc
    51181 agtgtttctc ctttcgcccg ccagaataaa aagcaaccgt ttacgttaat gaaactgtct
    51241 aaacacatgc tcgtggagtg ccagtttttg cgaggaaggg gagttagaga caggaattgc
    51301 ggaggcaatc acataatcgt gcttaaacca cgaaaatgac aagagaacct gacaatggaa
    51361 ttatctgtaa ttttctcata ttgatatttc attaatcatt ctggcatgtc gcactctcgc
    51421 atttaatcgt ttttatctgg atagcgctct tttgatcggc aatgttgaaa taatgaaagc
    51481 ggcatagatt gaggggcaat aatttgaact cagtcgcaaa attataatta aaacggtcat
    51541 gctacctaat atatgcccgt ttattgcatc gcgaaatttg agaagacata actcattaaa
    51601 tgaaagggat gcaaatgaat aagtttatcg ctgctgaagc tgcggaatgt ataggctgtc
    51661 atgcttgtga aattgcctgt gcggtggcac acaatcaaga aaactggccg ctgagtcaca
    51721 gtgactttcg accgcgtatc cacgttgtag ggaaaggcca ggctgcgaat ccggtggcct
    51781 gccatcactg caacaatgcc ccttgtgtta cggcttgtcc ggttaatgct ctgactttcc
    51841 agtccgatag cgtacaactg gacgagcaaa aatgtattgg ctgtaaaaga tgcgcaatcg
    51901 cttgcccctt tggcgtcgtt gagatggtcg atacgattgc gcagaaatgc gacctttgta
    51961 accagcgcag ttccggcacg caagcctgta ttgaagtctg cccaacacag gcgttacgac
    52021 tgatggacga taaagggtta cagcggataa aggtggcccg ccagcgcaaa acggcagcag
    52081 gaaaagcgtc atcagacgct cagccatctc gcagtgcagc gttgctcccc gttaactcgc
    52141 gtaaaggcgc agataaaatt tcagcgagtg aacggaaaac ccactttggc gaaatctatt
    52201 gcgggctgga tccactgcaa gcgacttatg agagtgaccg ctgtgtttat tgtgccgaaa
    52261 aagctaactg caactggcat tgcccgctgc ataacgctat tccggattac atccgtctgg
    52321 tacaggaagg aaagattatt gaagcggcag aactttgcca ccagaccagt tccttaccag
    52381 aaatctgcgg cagggtatgc ccacaggacc gtctttgtga aggcgcatgt actttgaaag
    52441 atcactcagg cgcagtctct atcggtaatc tggaacgcta catcaccgat accgcgctgg
    52501 cgatgggctg gcgtcccgat gtcagcaaag ttgttccccg tatcgaaaaa gtggcggtga
    52561 ttggcgctgg gccagcaggg ttagggtgtg ctgatattct ggcgcgcgcg ggagttcagg
    52621 ttgatgtctt tgagcgccat ccagaaattg gcggtatgct gacttttggc attcctcctt
    52681 tcaaactcga taaaacggta ttaagccagc ggcgagagat attcaccgca atgggaatcg
    52741 actttcatct taactgtgaa attggccgcg atattacctt tagcgattta acttctgaat
    52801 atgatgcagt tttcatcggc gtggggactt acgggatgat gcgagcagat ctgccgcatg
    52861 aagatgcgcc cggtgtcatt caggctctac cgttcctgac tgcccatacc cgccagctca
    52921 tgggattgcc ggagtctgaa gagtatccgc tgacggacgt ggaaggtaag cgagtcgtgg
    52981 tattgggcgg tggcgatacg acaatggatt gtttgcggac ttccattcgc ctcaatgctg
    53041 ccagcgtgac ctgcgcgtat cgtcgtgatg aagtcagtat gccgggctcg cgcaaagagg
    53101 tggtcaatgc gcgcgaggaa ggtgtcgagt ttcagttcaa tgttcagccg caatatatcg
    53161 cttgtgacga agatgggcgt ttaactgcag tgggcctgat tcgtaccgcc atgggggagc
    53221 aggggccgga tggtcgccgt cgtcctcgtc cggtagcggg ttcagagttt gaattgcccg
    53281 ccgatgttct gattatggcc tttggtttcc aggcgcatgc catgccgtgg ttgcagggca
    53341 gcggaattaa actcgataaa tggggcctga ttcaaaccgg tgacgtcggg tatttaccta
    53401 cccagacgca tctgaaaaaa gtctttgctg gtggtgatgc agttcatggc gcggatctgg
    53461 ttgtcactgc catggccgca ggaaggcagg cggcgcgcga tatgttaact ctgtttgata
    53521 cgaaggcatc gtgatgaaat cgttaattat cgttaatccg gctgactgta ttggctgccg
    53581 tacttgtgaa gtagcctgtg tggtcgctca cccttcagaa caggagttga atgccgatgt
    53641 ctttttaccc cggctgaagg tacagcgact ggacatcatc agtgcgccgg tgatgtgcca
    53701 tcagtgtgaa aacgctcctt gtgttggcgc ttgccccgtg caggcgctga cgatgggcga
    53761 gcaggtggtg caggccaatt ctgcccgttg tattggctgt cagagctgcg tcagtgcgtg
    53821 tccgtttggg atgatcacca ttcagtcgtt gccgggggat acccggcaac aaatcgtgaa
    53881 atgcgatctc tgtgaacagc gggaagaggg gccagcctgc gttgaatctt gcccgacaca
    53941 ggcgctgcag ttgcttactg aaagagaact caggcgactc cgccagcagc gtattgttgc
    54001 cagcggtgag aatcctctct gaaagaaatc tccgcgctga aaggcgcgga gaatctatta
    54061 gattgcccaa ccacccgcgt aaaatgcgac cagtgcggca gtaatgatca ccgtaccaat
    54121 attcagcttt tgccattcgc gtgcaaagac gcggcctacg accagtgtca caaagcccag
    54181 cataataccg gtaacgatat tacaagtcag aacgatgaac acggcacaca ccaggccagc
    54241 catagcgtca ataaaatcat tgaaatccag cttcgagacg ttacttaaca tcagcaaacc
    54301 cacgtacatc agagcggggg cagtggcgta accagggatc aaaaatgaca gcggtgataa
    54361 aaacagaatt aacaggaata acgcccccac tacggttgcg gtcaaccctg ttttaccccc
    54421 ggcggcggtt cctgccgctg attcgatata aaccgctgcg ggcgctgcgc ccaccaggcc
    54481 ggagaatatt gaacttactg agtcactggt cagggctttg ccgccgttga tgatctggtt
    54541 gtctttatcc aacaaattcg cctgaccggc gacagcacgg atggtgccag tagcgtcgaa
    54601 cactgcggtc atcaccaatg ccagtacact cggaagtaca gttggctgga gtgcgcccat
    54661 aatatcgagg ctgaaaatca gagacttacc atcttcgcca gtcaggcttg gcatcgccac
    54721 cagaccgtgg tatttcaccg ctgggtcaaa gattaagccg atgatcgaaa ttgcaataat
    54781 caccaacaag atcccgccgg gtacgcgaca cttctccaga ccgaagatga ccgccagccc
    54841 cagtaagctc atcatcaccg ggaaggaggt aaacgcaccg agcgccactg gcaagccttc
    54901 aatcgggttt ttgataacca tacccacacc gttagcagca atcagcagca gaaacagccc
    54961 gataccgata cctgtaccgt gagcgatacc catcggcaaa ttacgtaaga tccaggtacg
    55021 cacaccggtt acggaaatgg cggtgaagat gacgcccatc agaaataccg cgcccagtgc
    55081 gacaggaacg ctaatttgtt gcccgagtac cagactgaat gcggtaaacg ccgtcaagga
    55141 aatcgcgcaa ccaatcgcca ttggcaaatt agcccataat cccatcagca acgagccgaa
    55201 gcccgcgacc agacaggtgg caacaaacac agctgcggga ggaaaacctg ctttgcccag
    55261 cattcccgga acgacgataa cggaataaac catggccaga aaggtcgtta agccagccag
    55321 tacttcctga cgaacggtac tgccacgagc ggtaatttta aaataattat ccagcgcgcc
    55381 ctgtggcttt ggtgcgtccg gtgtttgtag gatgtctcca gacataatga tgtcctcacg
    55441 aggatgttaa agttttatta ttagttgcgt tcgtacccca gacgaccatc aacgtaggtg
    55501 cggtagatcg aacggtcatc gcccaacgtc atcatcacga acaatttgtc gactaaagaa
    55561 acagagttgt catagcgcag ctgctgtagc ggagtggcgg tgggttccat caccacgaaa
    55621 tcagcctctt tgctaggtaa aaagttgcca atcaaatcgt caaggcccag agatttcgct
    55681 ccgccgagcg tggccaggta aaacgcttca tatgccgaga ggcgatagcc ttgtaattgc
    55741 aatactttgt aggcttcgtt cagcgtttgc agcatgttga aagtggttcc ggcaccgata
    55801 tccgttccca tgcccacttt aactttcttc tgccatgctt ttttcaagtt gaataagccg
    55861 ctgccgaggt aaaggttgga ggtcggacag aaagcaatgc tggatttggt ttcgctgaga
    55921 cgatcccact ctttttcttc gagatggacg cagtgagcaa agacacagtt tttaccggtc
    55981 agaccgtact gatggtaaac atcca
//
LOCUS       DADLSA010000010        50019 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.010, whole genome shotgun sequence.
ACCESSION   DADLSA010000010 DADLSA010000000
VERSION     DADLSA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.010"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..318)
                     /locus_tag="JN691_000630"
     CDS             complement(<1..318)
                     /locus_tag="JN691_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HAY7242151.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIH"
     gene            complement(335..595)
                     /locus_tag="JN691_000631"
     CDS             complement(335..595)
                     /locus_tag="JN691_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY7242152.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(586..1856)
                     /locus_tag="JN691_000632"
                     /pseudo
     CDS             complement(586..1856)
                     /locus_tag="JN691_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(1934..2299)
                     /gene="queD"
                     /locus_tag="JN691_000633"
     CDS             complement(1934..2299)
                     /gene="queD"
                     /locus_tag="JN691_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAY7242153.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            2615..4414
                     /gene="cysJ"
                     /locus_tag="JN691_000634"
     CDS             2615..4414
                     /gene="cysJ"
                     /locus_tag="JN691_000634"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAY7242154.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            4414..6126
                     /gene="cysI"
                     /locus_tag="JN691_000635"
     CDS             4414..6126
                     /gene="cysI"
                     /locus_tag="JN691_000635"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAY7242155.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            6200..6934
                     /gene="cysH"
                     /locus_tag="JN691_000636"
     CDS             6200..6934
                     /gene="cysH"
                     /locus_tag="JN691_000636"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY7242156.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            7198..7350
                     /locus_tag="JN691_000637"
     CDS             7198..7350
                     /locus_tag="JN691_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7242157.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            7544..8695
                     /locus_tag="JN691_000638"
     CDS             7544..8695
                     /locus_tag="JN691_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HAY7242158.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKEGSDDYHLLCWHLLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     EFKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPRIEIPTFWDDDEGVELIKQLSW
                     YISATVVLADWTGSSTRFFPRVAQAMDIEDYWQKALVQAQNALTVFPPQAETAPFTGI
                     NTLFPFIENTTPLQQKVLDLDINQPGPQLFILEDVTGAGKTEAALILAHRLMAAGNAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPEVFPAPAEINRRNTVRFSRK"
     gene            complement(8781..9818)
                     /gene="iap"
                     /locus_tag="JN691_000639"
     CDS             complement(8781..9818)
                     /gene="iap"
                     /locus_tag="JN691_000639"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAY7242159.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTHDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            10070..10978
                     /gene="cysD"
                     /locus_tag="JN691_000640"
     CDS             10070..10978
                     /gene="cysD"
                     /locus_tag="JN691_000640"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAY7242160.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVTL
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            10980..12407
                     /gene="cysN"
                     /locus_tag="JN691_000641"
     CDS             10980..12407
                     /gene="cysN"
                     /locus_tag="JN691_000641"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAY7242161.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSLGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            12407..13012
                     /gene="cysC"
                     /locus_tag="JN691_000642"
     CDS             12407..13012
                     /gene="cysC"
                     /locus_tag="JN691_000642"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAY7242162.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            13062..13385
                     /locus_tag="JN691_000643"
     CDS             13062..13385
                     /locus_tag="JN691_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAY7242163.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            13579..13890
                     /gene="ftsB"
                     /locus_tag="JN691_000644"
     CDS             13579..13890
                     /gene="ftsB"
                     /locus_tag="JN691_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAY7242164.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMIRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            13909..14619
                     /gene="ispD"
                     /locus_tag="JN691_000645"
     CDS             13909..14619
                     /gene="ispD"
                     /locus_tag="JN691_000645"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAY7242165.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSENSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            14619..15098
                     /gene="ispF"
                     /locus_tag="JN691_000646"
     CDS             14619..15098
                     /gene="ispF"
                     /locus_tag="JN691_000646"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAY7242166.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTFGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            15095..16144
                     /gene="truD"
                     /locus_tag="JN691_000647"
     CDS             15095..16144
                     /gene="truD"
                     /locus_tag="JN691_000647"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAY7242167.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            16125..16886
                     /gene="surE"
                     /locus_tag="JN691_000648"
     CDS             16125..16886
                     /gene="surE"
                     /locus_tag="JN691_000648"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAY7242168.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            16880..17506
                     /gene="pcm"
                     /locus_tag="JN691_000649"
     CDS             16880..17506
                     /gene="pcm"
                     /locus_tag="JN691_000649"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAY7242169.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            17646..18785
                     /gene="nlpD"
                     /locus_tag="JN691_000650"
     CDS             17646..18785
                     /gene="nlpD"
                     /locus_tag="JN691_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAY7242170.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            18848..19840
                     /gene="rpoS"
                     /locus_tag="JN691_000651"
     CDS             18848..19840
                     /gene="rpoS"
                     /locus_tag="JN691_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAY7242171.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSCMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(19960..20367)
                     /locus_tag="JN691_000652"
     CDS             complement(19960..20367)
                     /locus_tag="JN691_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY7242172.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            20514..21107
                     /locus_tag="JN691_000653"
     CDS             20514..21107
                     /locus_tag="JN691_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY7242173.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            21107..22534
                     /locus_tag="JN691_000654"
     CDS             21107..22534
                     /locus_tag="JN691_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAY7242174.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGEEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRSKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTTMLAARK"
     gene            22545..22781
                     /locus_tag="JN691_000655"
     CDS             22545..22781
                     /locus_tag="JN691_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242175.1"
                     /translation="MICPRCADKQIEVMVKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(22821..24185)
                     /locus_tag="JN691_000656"
     CDS             complement(22821..24185)
                     /locus_tag="JN691_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HAY7242176.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSCGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGVVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTGDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(24280..25050)
                     /locus_tag="JN691_000657"
     CDS             complement(24280..25050)
                     /locus_tag="JN691_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HAY7242177.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYNYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDA"
     gene            complement(25055..25693)
                     /locus_tag="JN691_000658"
     CDS             complement(25055..25693)
                     /locus_tag="JN691_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY7242178.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(25690..26952)
                     /locus_tag="JN691_000659"
     CDS             complement(25690..26952)
                     /locus_tag="JN691_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HAY7242179.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMHHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVAHCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(26949..27721)
                     /locus_tag="JN691_000660"
                     /pseudo
     CDS             complement(26949..27721)
                     /locus_tag="JN691_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            27887..28684
                     /locus_tag="JN691_000661"
     CDS             27887..28684
                     /locus_tag="JN691_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7242180.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRINVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GSCIYLDAGTTTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(28735..29391)
                     /gene="pphB"
                     /locus_tag="JN691_000662"
     CDS             complement(28735..29391)
                     /gene="pphB"
                     /locus_tag="JN691_000662"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAY7242181.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGDEYLFGKEIAESELL
                     CPVYRMQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(29497..32058)
                     /gene="mutS"
                     /locus_tag="JN691_000663"
     CDS             complement(29497..32058)
                     /gene="mutS"
                     /locus_tag="JN691_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAY7242182.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNGPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            32134..32313
                     /locus_tag="JN691_000664"
     CDS             32134..32313
                     /locus_tag="JN691_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242183.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            32345..32698
                     /locus_tag="JN691_000665"
     CDS             32345..32698
                     /locus_tag="JN691_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAY7242184.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(32735..34803)
                     /gene="flhA"
                     /locus_tag="JN691_000666"
                     /pseudo
     CDS             complement(32735..34803)
                     /gene="flhA"
                     /locus_tag="JN691_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(34877..35887)
                     /gene="hypE"
                     /locus_tag="JN691_000667"
     CDS             complement(34877..35887)
                     /gene="hypE"
                     /locus_tag="JN691_000667"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAY7242185.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(35884..37005)
                     /gene="hypD"
                     /locus_tag="JN691_000668"
     CDS             complement(35884..37005)
                     /gene="hypD"
                     /locus_tag="JN691_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAY7242186.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(37005..37277)
                     /gene="hypC"
                     /locus_tag="JN691_000669"
     CDS             complement(37005..37277)
                     /gene="hypC"
                     /locus_tag="JN691_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAY7242187.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(37268..38140)
                     /gene="hypB"
                     /locus_tag="JN691_000670"
     CDS             complement(37268..38140)
                     /gene="hypB"
                     /locus_tag="JN691_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAY7242188.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYRHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(38144..38494)
                     /gene="hypA"
                     /locus_tag="JN691_000671"
     CDS             complement(38144..38494)
                     /gene="hypA"
                     /locus_tag="JN691_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY7242189.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKCVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            38706..39167
                     /gene="hycA"
                     /locus_tag="JN691_000672"
     CDS             38706..39167
                     /gene="hycA"
                     /locus_tag="JN691_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAY7242190.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            39292..39903
                     /locus_tag="JN691_000673"
     CDS             39292..39903
                     /locus_tag="JN691_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7242191.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            39900..41724
                     /gene="hycC"
                     /locus_tag="JN691_000674"
                     /pseudo
     CDS             39900..41724
                     /gene="hycC"
                     /locus_tag="JN691_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
     gene            41727..42650
                     /gene="hycD"
                     /locus_tag="JN691_000675"
     CDS             41727..42650
                     /gene="hycD"
                     /locus_tag="JN691_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAY7242192.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            42668..44377
                     /locus_tag="JN691_000676"
     CDS             42668..44377
                     /locus_tag="JN691_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAY7242193.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFSNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDGMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            44387..44929
                     /gene="hycF"
                     /locus_tag="JN691_000677"
     CDS             44387..44929
                     /gene="hycF"
                     /locus_tag="JN691_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HAY7242194.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            44929..45696
                     /gene="hycG"
                     /locus_tag="JN691_000678"
     CDS             44929..45696
                     /gene="hycG"
                     /locus_tag="JN691_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HAY7242195.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            45693..46103
                     /locus_tag="JN691_000679"
     CDS             45693..46103
                     /locus_tag="JN691_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HAY7242196.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            46096..46566
                     /gene="hycI"
                     /locus_tag="JN691_000680"
     CDS             46096..46566
                     /gene="hycI"
                     /locus_tag="JN691_000680"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HAY7242197.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            46609..47360
                     /locus_tag="JN691_000681"
                     /pseudo
     CDS             46609..47360
                     /locus_tag="JN691_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            47533..47844
                     /locus_tag="JN691_000682"
     CDS             47533..47844
                     /locus_tag="JN691_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAY7242198.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            47841..48260
                     /locus_tag="JN691_000683"
     CDS             47841..48260
                     /locus_tag="JN691_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7242199.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(48375..49799)
                     /locus_tag="JN691_000684"
     CDS             complement(48375..49799)
                     /locus_tag="JN691_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY7242200.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(49808..>50019)
                     /locus_tag="JN691_000685"
     CDS             complement(49808..>50019)
                     /locus_tag="JN691_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAY7242201.1"
                     /translation="GLFTSVQFFDPANPMSIVWVFAVMALAVMLSFILTLLLGFEDIP
                     VEEAAAQARKHQSVQPTVAKEVSLN"
BASE COUNT        11805 a        12977 c        13456 g        11781 t
ORIGIN      
        1 atgaatacag aaaaaaactt ccgctcttgc cgcttttagc atcgatgctt tggtgctgat
       61 aattccatcc gcctcggtca ccagtttcaa aaactgaatc accacttctt tatttgacgc
      121 gccttccagc aaatcaacat gaataaaagc atatttcccg gcgttcttaa tctttttaac
      181 gatattactg atggtgcaga tattgccgta caacacggaa ataaactggc attcagaatc
      241 gatggccagt tgcaggctgg cattatcttt aacggcagca atcaccggat tctggcggag
      301 caggtgtaag aggggcataa attatcctta ttatttatcc aaagcggtag gtgatgccga
      361 atccggaagg cggatagcgc cactgttgta gtgtggattc gtcgcacagc aaacggcagg
      421 tgccacactc caggcagccg cgatagtcaa tacgtaagtc accttcctgt gtgagagaaa
      481 aaagacctac cggacaggcg ttaatcaacc gttgtaccat ctgacgctca acggagtcag
      541 ccggaacaat gtgcggcgca tcagcaacgc gccagagatt acgggctaca gacatcgcag
      601 actcctgata acatcgcccg ccagatgcca caggccatga cgacgtaaat gacgccagaa
      661 taactggcgc agcggtggaa caggtttatc accctgatcc cataaatcgc gggaaatatc
      721 ctgcattaac gcaggccacg cccgatacca gccaggggtt gcaaaagcgc cggaacatgc
      781 tgataacgtt gtagaacatc ccatagcagg ctgcgctcta cgttgtgatg ataaagcgca
      841 aacagatttt gcggctcgcg gtgctggcag gcgcttatta gcgtttgtgc cgccgcctgt
      901 gcgccagtca gcgccatatc catgccgcgc acggaaattc cggtattgac gcaactgcgc
      961 aacgcatcgc ccaccagcag ccagccgtta ccggcgtatt gcaccggcat actgtgcaag
     1021 ccaccttctg gcaccagatg cgcaccatac tccagtgatt ccgtgttttt gataagcggg
     1081 cgcactgccg gatgcgcttt aaagcgagtc agcagctcgc ttgccggaac acgactttgc
     1141 gtaagggaag agagcgggca aacaatccct aacgagagcg tttgttgatt agtataaaga
     1201 aatgcgccgc cgggtaagtc atcacagatt ccgccgctga acagcaacgc tgcgccttcg
     1261 ttattctcca gatgaaaacg ttcttcaata gcggatgttt ccagcgacag cacttctttg
     1321 atccccaacg ccatcgcttc gccagcagga cgagtcacta acccgtgacg ttcagccagg
     1381 acgctgttgg caccttctgc cagcaccaca taacgggcgc ggagaatatc gtcaccacaa
     1441 ataacgccac agactctgcc gttttcttca tacagtgcat ccaccgtcgc tccggggatg
     1501 cattcgacac cttctttttc ggcttcggca accagccacg gatcgaaccg tgcacgtaat
     1561 acactccagg attcaccgcc gggctgtaag ctggaaaacg tcgttgcgcc atccggcgtt
     1621 aacagggaaa ggctttcgtg agtgatgcgt cgttcaagag gcgcggtcag atgaaattgc
     1681 gggaggagtt ccgcgagtgc atgggtatat aaccgcccgc cggaaagatt tttgctgccg
     1741 gggatttcag cgcgttcgag taacaaaacg gataaatccg ctcgcgcgca gcgtaacgcg
     1801 caagcggtgc ctgcaatacc ggcaccaata ataataatgt cgcagtcgtc ttccatgtca
     1861 tgccccagat atccagaaca caacacccta acatagcgtt actaaaggga aattgaccgc
     1921 cgacacactc ttatcattcg ccgcgataga tacaccctgc ggtgcaggtt tcttttacca
     1981 tcaccgcact taacagcggc acaacgggtt taacctgatc ccaaatccat tttgctaaaa
     2041 cttcgctggt tgggttttcc agacctggaa tatcattgag ataatggtga tcgaggcgct
     2101 cataagttgg tttaaacgcc gctttcagtt cagcgaaatc gataatccag ccagtatgcg
     2161 gatcgacttc cccggtaatt tccagtcgca ccataaagga atgcccgtgc aggcgaccac
     2221 atttatgccc ttccgggaca tgtggtaagc ggtgagcggc ttcgaaggtg aaatctttaa
     2281 ataacgtggt ggacatcata atttctctac aggacgataa aaccgccgta gagtaccgga
     2341 aaggctgttt tttcgcatta tctaaaacga ctttgttgcg caaaatcgct gatttatctt
     2401 aatgattggc taaattcatt tgtttttcat taggttggtt aatctatttt gttgttaaag
     2461 actattgcta aaacaggtta gtcgatttgg ttattagtta tcgctatccc gtctttaatc
     2521 cacaccgttc accccgttaa ccttaccttc tcttctgttt tatgggcgct gacagggcgc
     2581 agaaacagct ttgcttactg gaacataacg acgcatgacg acacaggtcc caccttccgc
     2641 gttgcttccg ttgaacccgg agcaactggc acgccttcag gcggccacga ccgatttaac
     2701 gcctacccag cttgcctggg tttctggcta tttctggggc gtgctcaacc agcagcctgt
     2761 tgcgcttgca gtgacgccag cgcccgccgc agaaatgccg ggtataacga ttatctctgc
     2821 ttcgcaaacc ggcaatgcgc gccgggttgc tgaagcatta cgcgatgatt tattagcagc
     2881 aaaactgaac gttaagctgg tgaacgcggg cgactataaa ttcaaacaaa tcgccagcga
     2941 aaaactgctc atcgtagtga cgtcaacgca aggggaaggg gaaccgccgg aagaagccgt
     3001 cgcgctgcat aagttcctgt tctccaaaaa agcgccaaag ctggaaaaca ccgcgtttgc
     3061 cgtgtttagc ctcggcgata gctcttatga atttttctgc cagtccggga aagattttga
     3121 cagcaagctg gcggaactgg gtggtgaacg cctgctcgac cgtgtcgatg ctgatgttga
     3181 ataccaggct gctgccagcg agtggcgcgc ccgcgtggtt gatgcgctta aatcgcgtgc
     3241 gcctgtcgcg gcaccttcgc aatccgtcgc tactggcgcg gtaaatgaaa tccacaccag
     3301 cccgtacagc aaagacgcgc cgctggtggc tagcctctct gttaaccaga aaattactgg
     3361 gcgtaactct gaaaaagacg ttcgccatat cgaaattgac ttaggcgact cgggcctgcg
     3421 ttaccagccg ggtgacgcgc tgggcgtctg gtatcagaac gatccggcac tggtgaaaga
     3481 acttgtcgaa ctgctgtggc tgacaggcga tgaacctgtc actgtcgagg gcaaaacgtt
     3541 gcctctgaac gaagcgctac agtggcactt cgaactgacc gtcaacaccg ccaacattgt
     3601 tgagaattac gccacgctta cccgcagtga aacactgctg ccgctggtgg gcgataaagc
     3661 gaagttacag cattacgccg cgacgacgcc gattgttgac atggtgcgtt tctccccggc
     3721 acagcttgat gccgaagcgc taattaatct gctgcgcccg ctgaccccgc gtctctattc
     3781 cattgcctcc tcgcaggcgg aagtcgagaa cgaagtacac gtcaccgttg gtgtggtgcg
     3841 ttacgacgtg gaaggccgcg cccgtgccgg tggtgcctcc agcttcctcg ctgaccgcgt
     3901 ggaagaagag ggcgaagtcc gcgtatttat cgaacataac gataacttcc gcctgcccgc
     3961 taacctggaa accccggtga ttatgattgg cccaggcacc ggtattgcgc cgttccgcgc
     4021 ctttatgcag caacgcgccg ctgacgaagc gccaggtaaa aactggctgt tctttggcaa
     4081 tccgcatttt acggaagact tcctgtatca ggtggagtgg cagcgctacg tcaaagaggg
     4141 tgtgctgacg cggatcgatc tcgcctggtc gcgcgaccaa aaagaaaaag tttacgtaca
     4201 agataaactg cgcgaacagg gcgcggagct gtggcgctgg atcaatgacg gtgcccacat
     4261 ttatgtctgc ggcgacgcta atcgcatggc gaaagacgtt gagcaggcac ttctggaagt
     4321 gattgccgaa tttggtggca tggacaccga agcggcggat gaatttttaa gtgagctgcg
     4381 cgtagagcgt cgttatcagc gagatgtcta ctaatgagcg aaaaacatcc agggccttta
     4441 gtggtcgaag gaaaactgac agacgccgag cgcatgaagc ttgaaagcaa ctacctgcgc
     4501 ggcaccattg cggaagattt aaacgacggt ctgaccggcg gctttaaggg cgataacttc
     4561 ctgctgatcc gcttccacgg catgtatcag caggatgacc gcgacatccg cgccgaacgt
     4621 gctgaacaga agctggagcc gcgccacgcg atgctgttgc gctgccgtct gccgggtgga
     4681 gttatcacca ccaaacagtg gcaggcgatc gataaatttg ccggtgaaaa caccatctat
     4741 ggcagcattc gcctgaccaa ccgccagacg tttcagttcc acggcattct gaaaaagaac
     4801 gtcaaaccgg tgcaccagat gctgcactcg gtcggtcttg atgcgctggc gactgctaac
     4861 gacatgaacc gtaacgtact ctgcacctcg aacccttacg agtcgcagct gcacgcagaa
     4921 gcgtacgagt gggcgaagaa gatttctgaa catctgctgc cgcgtacccg cgcgtatgcg
     4981 gagatctggc tcgatcagga aaaagtcgcc accaccgatg aagaaccgat ccttggtcag
     5041 acctacctgc cgcgtaagtt caaaaccacg gtagtgatcc cgccgcagaa cgatattgat
     5101 ctgcacgcca acgacatgaa cttcgtggcg atcgcggaaa acggcaagct ggtgggcttc
     5161 aacctgctgg tgggcggtgg gctttctatc gaacacggta acaagaaaac ctacgcccgc
     5221 acggcgagtg agtttggcta tctgccgcta gagcatacgc tagcggtggc ggaagccgtc
     5281 gtgacaactc agcgtgactg gggtaaccga accgatcgta aaaatgccaa aaccaaatac
     5341 acactggagc gcgtgggggt tgagacgttt aaagcggaag tggagcgtcg cgcggggatc
     5401 aaatttgaac cgatccgccc gtatgaattt acagggcgcg gcgatcgtat tggctgggtt
     5461 aagggcattg atgataactg gcacctgacg ctgtttatcg aaaatggtcg catccttgat
     5521 tatccggggc gtcctctgaa aactggcctg ctggagatcg cgaagatcca caaaggtgat
     5581 ttccgcatta cggcgaacca gaatctgatc atcgccggtg tgccggaaag cgagaaagcg
     5641 aagatcgaga aaattgccaa agagagcggg ttaatgaatg ccgtcacgcc gcagcgtgaa
     5701 aactcgatgg cctgcgtgtc attcccgact tgcccgctgg cgatggcgga agcggagcgt
     5761 ttcctgccgt cttttatcga caacatcgat aatttaatgg cgaaacatgg tgtcagcgat
     5821 gagcatatcg tgatgcgtgt aacaggctgc ccgaacggtt gtggtcgcgc gatgctggcg
     5881 gaagtgggcc tggtgggtaa agcaccgggt cgctacaacc tgcatcttgg cggcaaccgc
     5941 attgggacac gtatcccacg gatgtataaa gaaaacatca ccgagccgga aatcctggcg
     6001 tcgcttgatg aactgatagg gcgctgggcg aaagagcgcg aagcgggtga aggcttcggc
     6061 gactttacgg tgcgtgcggg catcattcgc ccggtgctcg atccggcgcg cgatttgtgg
     6121 gattaaccat cagcccggtc ttgtaggcct gataagacgc gtgagcgtcg catcaggcaa
     6181 ggcaaacagt gaggaatcta tgtccaaact cgatctaaac gccctgaacg aactgccgaa
     6241 ggtagatcgc attctggcgc tggcagaaac taacgccgaa ctggaaaaac tggacgctga
     6301 aggccgcgtt gcctgggcgc tggataatct gcccggtgaa tatgtacttt cttccagctt
     6361 tggcattcag gcggcggtga gcctgcatct ggtgaatcag attcacccgg atattccggt
     6421 gatcctcacc gacaccggct atctgttccc ggaaacctac cgctttattg acgagttaac
     6481 ggacaaactc aagctcaacc tgaaagtgta ccgcgctacc gaaagcgccg cctggcagga
     6541 agcacgctac ggcaaactgt gggaacaggg cgttgaaggc attgaaaagt acaatgacat
     6601 caacaaagtc gaaccgatga accgggcgct gaaagaactg aatgcgcaaa cgtggtttgc
     6661 tggcctgcgg cgtgaacaat ccggcagccg tgccaattta ccggtgctgg caattcagcg
     6721 tggcgtattt aaagtgctgc cgattatcga ctgggataac cgaactattt atcagtacct
     6781 gcaaaaacat ggcctgaaat atcacccatt atgggacgaa ggatatttat cggtgggcga
     6841 tacccataca acccgtaaat gggaacccgg catggcggaa gaagaaacgc gtttctttgg
     6901 cttaaaaagg gaatgtggat tacacgaagg gtaaatattg ccggataaat taaacgcctc
     6961 tgtcagaaat gatgggggcg tttttatttt gcgagcagca ttccggcatt taaaggtgaa
     7021 catcgcaggg aaaattaaca ggtatagaat ggcgttgggt gcttgaggct gtctggcgtt
     7081 aagcattcgc gaggttccag atggacaaaa gccccaggcg atatttctat caacctgagg
     7141 ctcgcgttcg aacgtagcaa ttcggatgtt agtctcttct ttgcagggag gcaagacatg
     7201 ctgacaaaat atgcccttgt ggcaatcatc gtactgtgtt gtacagtact gggattcacg
     7261 ttgatggtag gtgactcgtt atgtgagttg agtatcagag aacgtggtat ggagtttaag
     7321 gcagttctcg cttacgaatc gaagaagtag ccaccacgcg ggggtaaaac cccgcgtact
     7381 gaattgtgat ggtttggtct cctgcaccct tttttaaaat ttatattttt tatgtgaata
     7441 tatagtctct atttattatt atgaatttca aatgaaaatc attaatttat tcataatgta
     7501 aatgttttat tggctgatat atatcacaga taatttttaa aatatgcgta aatatccttt
     7561 aagtttactg aaggataaaa atattgtgac tttctttgat ttctggggaa aaacccgacg
     7621 tggcgagaaa gagggtagcg acgactatca ccttctttgc tggcatttgc tggatgtggc
     7681 cgcaatgggc tatttaatgg ttaaaagaaa ttgcttcggg ctggctgatt attttcgtca
     7741 attagggatt tctgacaagg aacaggcagc tcaatttttc gcctggttgc tgtgctggca
     7801 cgatattgga aaatttgccc gctcttttca acaactttac ctgccccctg aattcaagat
     7861 tcaggaaggt gccagaaaaa attacgaaaa aatctctcat tcaacgctgg gctactggtt
     7921 gtggaatcat tatttaagtg aatgtcagga gttacttcct tcatcttcgc tctctccgcg
     7981 caaacttagg cgagtgatag aaatgtggat gcccgtgacg acagggcatc atggtcggcc
     8041 ccctgatcgt atggatgaat tggataattt tctgcctgaa gacaaagctg cagcacgaga
     8101 ttttctcctt gaaatcaagc cactgtttcc gcgaatagag atccccacat tctgggatga
     8161 tgacgagggc gttgaactta taaaacaact ttcctggtat atctctgcaa ccgtcgtact
     8221 tgcagactgg acagggtcgt caacgcgatt ttttccacgc gtcgcacagg caatggatat
     8281 tgaagattac tggcaaaagg ctttagttca ggctcaaaac gcattaaccg tctttccgcc
     8341 acaagcagaa accgcgcctt tcaccggaat taatacgctg tttcctttta ttgagaatac
     8401 gacaccgtta cagcaaaagg tgctggatct ggatatcaat cagccaggac cacagttgtt
     8461 tattctggaa gatgtgaccg gcgcaggtaa aacagaagcg gcgcttatcc tggcgcacag
     8521 gttgatggct gcgggtaacg ctcaggggct gttttttggc ctgccaacaa tggcaacggc
     8581 caatgccatg tacgatcgac tggtcaaaac ctggcttgct ttctattcgc cagaggtgtt
     8641 ccccgctcca gcggagataa accggcgcaa tacggtgcgg ttttcccgga agtaaaccgg
     8701 acgcactgga tgcgatgatg ggtatcactt agaattcccc gcccctgcgg tagaactccc
     8761 agctcccatt ttcaaaccca tcaagacgcc ttcgccagct ccttcaccaa cggtagcatt
     8821 atccgcataa cgtcacggca gcgacgttct attcttccag gaagtgcctt atcaatatgt
     8881 tgttgattat ccagtcttac gtcatgccag ctatttcccg ccgggaaggc aggtgttttt
     8941 gcgcgttgct gataaccatc cttatttcca agattccagt tagtcgcttc caccgaaagt
     9001 acagcaatgc ccgctttgtc gaatatttct gcgtcattac aacacccagt gcctttcgga
     9061 taatttttat tcaaacccgg attggtcgtt gcggctattc cgtgactgcg tgcaattgcc
     9121 agcgccctgt cgtgcgttaa tttccttact gcttcagggg tttttacacc gctgttgaaa
     9181 tacaatttat cgccaacaat taagttatcg agattaatca ccagcagcgt atttttcttt
     9241 tcggtgtcac tcatccgctt gagtaaattc tcagcgccta attttccttc ctcttcgccg
     9301 ctggtcgcca caaatcgaat accatactcg gtaggcgtat ttttcaggcg ttctgccagt
     9361 tccagcatga cacctaaacc tgcggcgtta tcatctattc cttgtaacgt cagtccgccg
     9421 agattggcat ctgcgtcagc atcgctcagc ggggcgtaag tatccagatg cgccataatg
     9481 atgatctgct gcggcgcttt gccttcatga gcggcaatca ccgtacttcc tgtcacgtta
     9541 tgccagttct tgcgattatc gcgggcggta taaatatacc gactattaaa tgtccgaata
     9601 tcactgcgat aacccatttg ctgaaactgt tggcgaatat aatcggcaga taacatttct
     9661 gctggagttc cggtcatgcg tcccggaaag aaagtagcaa tatgtcgtgc ctgggtatta
     9721 gcaaaatcgc caggtttagg tgacgaggcg tgtacgggga gaataaagca tacgccgagc
     9781 gccagggcag cggtacggtg gcgcaatgcg gaaaacatag tgagtcctta aataccatgc
     9841 aaattttttt accgccatag tatgaaactg ccgctgcgct aaaacaattt caaatcttcc
     9901 taaacgcccg aaatccggtg ccttaagcac tttttgatat tagctttgcc aaatcgttat
     9961 tccgttaagg aactactcat tctaattggt aatttcattc gttctcttac gctccctata
    10021 gtcgaaacat ctgatggcaa gaaaatagcg gtattgcaaa ggaacggtta tggatcaaaa
    10081 acgacttact cacctgcggc aactggaggc ggaaagcatc cacattattc gcgaggtggc
    10141 ggcagaattc tcaaatccgg tgatgctcta ctctatcggt aaagattcca gcgtcacgct
    10201 gcatctggcg cgcaaggcgt tttatccagg tacgctgcct ttcccgttgc tgcatgtcga
    10261 taccggctgg aaattccgcg agatgtatga gttccgcgat cgtacggcga aagcctacgg
    10321 ctgcgagctg ctggtgcata aaaacccgga aggcgtggcg atggggatta atccattcgt
    10381 gcacggcagc gcgaaacata ccgatattat gaaaactgaa ggcctgaaac aggcgctgaa
    10441 caaatacggt tttgatgccg ccttcggtgg tgcgcgccgt gacgaagaga aatcccgcgc
    10501 taaagagcga atctactctt tccgtgaccg cttccatcgc tgggatccga aaaatcagcg
    10561 cccggaactg tggcacaact acaacgggca aattaacaaa ggcgaaagca tccgcgtctt
    10621 cccgctctct aactggaccg agcaggatat ctggcaatac atctggctgg aaaatatcga
    10681 cattgtgccg ctgtatctcg ctgcggaacg tccggttctg gaacgcgacg gtatgttgat
    10741 gatgattgat gacaaccgta tcgacctgca accgggcgaa gtgatcaaaa aacggatggt
    10801 gcgtttccgt acgctgggct gctggccgct gaccggtgcg gtggagtcaa atgcacaaac
    10861 actgccggaa atcatcgaag agatgctggt ttccaccacc agtgaacgtc agggtcgcgt
    10921 gattgaccgc gaccaggcgg gatccatgga gctgaaaaaa cgtcaggggt atttttaaga
    10981 tgaacaccgt acttgcacaa caaatcgcca atgaaggcgg cgtcgaagcc tggatgattg
    11041 cgcaacaaca taaaagcctg ctgcgttttc tgacctgtgg tagcgtcgat gacggcaaaa
    11101 gtaccctgat tggtcgtctg ctgcacgata cccgccaaat ctacgaagat cagctctcat
    11161 cgctgcataa cgacagtaag cgtcacggca cccagggcga aaagctggat ctggctctgc
    11221 tggtggacgg cctgcaagct gagcgcgaac agggcatcac tattgatgtg gcctaccgct
    11281 atttctctac cgagaagcgt aaatttatta tcgccgacac cccagggcac gagcagtaca
    11341 ctcgcaatat ggcgactggc gcatcgacat gtgaactggc gatcttactg atcgatgccc
    11401 gtaaaggcgt gctcgatcaa acccgtcgtc acagttttat ctccacactg ttggggatca
    11461 aacatctggt cgtggcgatc aacaaaatgg atctggtgga ttacagtgaa gagacgttca
    11521 cccgtattcg tgaagattat ctgacctttg ccgggcagct gccgggtaat ctggatatcc
    11581 gctttgtgcc gctctccgca ctggaaggtg acaacgtggc ttcgcaaagt gaaagtatgc
    11641 cgtggtacag cggtccgaca ctgctcgaag tgctggaaac cgtggagatc cagcgagtgg
    11701 tggatgccca gccaatgcgc ttcccggtgc agtacgttaa ccgcccaaat ctcgattttc
    11761 gtggctacgc cggaatgctg gcatccggtc gcgtggaagt cgggcaacgt gtaaaagtgc
    11821 tgccctctgg tgtggaatca aacgtcgcgc ggatcgtgac ttttgatggt gatcgcgaag
    11881 aagcctttgc cggagaagcg atcaccctgg tgctgacgga tgagatcgat atcagccgtg
    11941 gtgatctgct gctggcggca gacgaagcgt taccggcggt gcagagtgcg tcggtggatg
    12001 tggtatggat ggcggaacag ccgctttccc tgggccagag ttatgacatc aaaattgccg
    12061 gtaagaagac gcgtgctcgt gttgatggca ttcgttatca ggttgatatt aataacctta
    12121 cccaacgcga agttgaaaac ctgccgctga acggcatcgg cctggtggat ctcacttttg
    12181 acgagccact ggtgttagat cgctatcagc aaaatccggt taccggtggg ctgattttta
    12241 tcgatcgcct gagcaatgtg accgtgggtg ccggtatggt gcacgagcca gttagccagg
    12301 caacagctgc gtcatctgaa ttcagtgcat tcgaactgga attgaatgcc ctggttcgtc
    12361 gccactttcc gcactggggc gcgcgcgatt tgctggggga taaataatgg cgctgcatga
    12421 cgaaaacgtc gtctggcata gtcatccggt cactgtgcaa caacgcgaac tacaccacgg
    12481 tcatcgtggt gtagtgctgt ggtttaccgg cctctccggg tccggtaaat caacggtcgc
    12541 cggggcgctg gaggaggcgt tacataaact cggcgtcagt acgtatctgc tggatggcga
    12601 caatgttcgc cacggattat gcagcgatct cggttttagc gatgccgatc gtaaagagaa
    12661 tatccgtcgc gtcggtgaag tggcgaattt gatggttgaa gccggactgg tggtgctgac
    12721 cgcatttatc tcgccacacc gcgccgaacg ccagatggtt cgcgaacgcg taggagaagg
    12781 gcgctttatt gaagtgtttg tcgatacgcc gctggcgatt tgcgaagccc gcgatccaaa
    12841 aggcttatat aagaaagcgc gtgccggtga actgcgcaac tttacgggaa tagattccgt
    12901 ttacgaagcg cctgaatcgg cagaaattca tctcaatggt gaacaattag taacaaattt
    12961 ggtacagcaa ttattagatc tgttgagaca gaacgatatt atcagatcct gagacaccac
    13021 cgggtttcca tgcccggttc gtcagagtaa caggatcaaa tatgcgcaat agccataaca
    13081 ttacactaac aaataacgac agccttactg aggatgaaga aactacatgg tcactgcctg
    13141 gtgccgtggt cggttttatc tcctggttat ttgcgctggc gatgccgatg ttgatttatg
    13201 gctctaacac gctgttcttc tttatctaca cctggccttt ctttctggca ctgatgcctg
    13261 tcgcggtagt ggtggggatt gcgctgcatt cattgatgga cggaaagcta cgctacagta
    13321 ttgttttcac cctggtgaca gtaggcatta tgtttggcgc actgtttatg tggctactgg
    13381 gctaatttgt actttcttgt ctcctctgtt cataattcaa accgtaacta ataatgagat
    13441 tatgttctgc acgccctggg tatacgtaac aatggacaaa tgtggtacat ttgcccgcgt
    13501 tgtcgcggta tccccaacag aggatgtaga gtcgtcttcg gatgcatggg atgatgatgc
    13561 cgtttttcag ggggcaggat gggtaaacta acgctgctgt tgctggctat tctggtctgg
    13621 ctacagtatt cgctgtggtt cggtaagaac ggtatacatg actatacccg cgtcaatgat
    13681 gatgtggcgg cactgcaagc tacaaacgcg aaacttaaag cgcgaaacga tcaacttttt
    13741 gccgaaattg acgatctcaa tggcggccag gaggcgctcg aagagcgtgc gcgtaatgaa
    13801 ctcagcatga tcaggccggg cgaaactttt tatcgtctgg tgcctgacgc gtcgaagcgc
    13861 gcacagtctg cggggcaaaa caatcgataa atcagcccgg gaattaacat ggcaaccact
    13921 catttggatg tttgcgccgt ggttccggcg gccggatttg gccgtcgaat gcaaacggaa
    13981 tgtcctaagc aatatctctc aatcggtaat caaaccattc ttgaacactc ggtgcatgcg
    14041 ctgctggcgc atccccgggt gacacgtgtc gtcattgcca taagtcctgg cgatagccgt
    14101 tttgcacaac ttcctctggc gaatcatccg caaatcaccg ttgtagatgg cggtgatgag
    14161 cgtgccgatt ccgtgctggc aggtctgaaa gccgctggcg atgtgcagtg ggtattggtg
    14221 catgacgccg ctcgtccttg tttgcatcag gatgacctcg cgcgattgtt ggcgttgagc
    14281 gaaaacagcc gcacgggagg gatcctcgcc gcaccagtgc gcgatactat gaaacgtgcc
    14341 gaaccgggca aaaatgccat tgctcatacc gttgatcgca acggcttatg gcacgcgctg
    14401 acgccgcaat ttttccctcg tgagctgtta catgactgtc tgacgcgcgc tctaaatgaa
    14461 ggcgctacca ttaccgacga agcctcggcg ctggaatatt gcggatttca tccgcagttg
    14521 gtcgaaggcc gcgcggataa cattaaagtc acgcgcccgg aagatttggc actggccgag
    14581 ttttacctca cccgaaccat ccatcaggag aatacataat gcgaattgga cacggttttg
    14641 acgtacacgc ctttggcggt gaaggcccaa ttatcattgg tggcgtacgc attccttacg
    14701 aaaaaggatt gctggcgcat tctgatggcg acgtggcgct tcatgcgttg accgatgcat
    14761 tgcttggcgc ggcggcgctg ggggatatcg gcaaactgtt cccggatacc gatccggcat
    14821 ttaaaggtgc cgacagccgc gagctgctac gcgaagcctg gcgtcgtatt caggcgaagg
    14881 gttatacctt tggcaacgtc gatgtcacta tcatcgctca ggcaccgaag atgttgccgc
    14941 acattccaca aatgcgcgtg tttattgccg aagatctcgg ctgccatatg gatgatgtta
    15001 acgtgaaagc cactactacg gaaaaacttg gatttaccgg acgtggggaa gggattgcct
    15061 gtgaagcggt ggcgctactc attaaggcaa caaaatgatt gagtttgata atctcactta
    15121 cctccacggt aaaccgcaag gcaccgggct gctgaaagcc aatccggaag actttgtggt
    15181 ggtggaagat ttgggctttg agcctgatgg tgaaggtgag catattctgg ttagaatcct
    15241 caaaaacggc tgcaataccc gttttgtggc ggatgcactg gcgaaattcc tgaaaattca
    15301 ttcccgtgaa gtcagcttcg ctgggcaaaa agacaaacat gctgtaacgg aacagtggtt
    15361 atgtgctcgc gtgccgggca aggaaatgcc cgatctgagc gcctttcaac tggaaggctg
    15421 ccaggtgctg gagtatgcgc ggcacaagcg caagctgcgt ttaggggcgc tgaaaggtaa
    15481 cgcctttacc ctggttctgc gcgaagtgag caatcgcgat gacgttgaac aacgtctgat
    15541 cgatatttgc gtaaaaggtg taccgaacta cttcggtgcc caacgttttg ggattggcgg
    15601 tagcaacttg cagggcgcgc tgcgctgggc gcaaaccaat actccggtgc gcgatcgcaa
    15661 taaacggagt ttctggttgt cggcagcccg cagtgcgttg tttaatcaga ttgttgctga
    15721 gcgcctcaaa aaagcagacg ttaatcaagt tgttgacggc gatgcgctac aattagccgg
    15781 acgtggtagc tggtttgtag caaccaccga agaactggcg gaattacagc gtcgcgtcaa
    15841 cgataaagag ctgatgataa ccgccgcatt gccgggcagt ggcgaatggg gaactcagcg
    15901 tgaagcgctg gcattcgaac aagcagctgt cgccgcagaa actgaattac aagctttact
    15961 ggtgcgcgaa aaagttgaag ccgcgcgcag agcgatgctg ctgtatccgc aacaattaag
    16021 ctggaactgg tgggatgacg tcaccgtaga gatccgtttc tggcttccgg cgggtagttt
    16081 tgcaaccagc gttgtcaggg aacttatcaa cacaacaggt gattatgcgc atattgctga
    16141 gtaatgatga cggggtacat gcacccggta tacaaacgct ggcgaaagcc ttgcgtgagt
    16201 ttgctgacgt tcaggtggtc gcccccgatc gtaaccgcag cggcgcttca aattctctga
    16261 cactggaatc ctccctgcgc acgtttacct ttgaaaatgg tgatattgct gtgcaaatgg
    16321 gaaccccgac cgattgtgtc tatcttggcg tgaatgctct gatgcgtccg cgcccggaca
    16381 ttgttgtgtc cggaattaac gccgggccga atctggggga tgatgttatt tattccggta
    16441 cggtagccgc cgcgatggaa ggccgtcatt taggttttcc ggcgctggcc gtctcgcttg
    16501 acgggcataa acattacgac actgccgcgg cggtaacctg ttcaattttg cgcgcactgt
    16561 gtaaagagcc gctgcgcacc ggacgtattc ttaatattaa cgttccggat ttacccttgg
    16621 atcaaatcaa aggtattcgc gtgacgcgct gcggtacacg acatccggca gatcaggtga
    16681 tcccgcagca agatccgcgc ggcaatacgc tgtactggat tggcccgccg ggcggtaaat
    16741 gtgatgccgg tccggggacc gattttgctg cggtagatga gggctatgtc tccatcacgc
    16801 cgctgcatgt ggatttaact gcgcatagcg cgcaagatgt ggtttcagac tggttaaaca
    16861 gcgtgggagt tggcacgcaa tggtaagcag acgcgtacaa gcacttctgg atcaattacg
    16921 tgcgcaaggt attcaggatg agcaggtgct gaatgcactt gccgccgtgc cgcgtgaaaa
    16981 attcgttgat gaagcgtttg aacaaaaagc ctgggacaat atcgccttgc cgataggtca
    17041 ggggcagaca atttcgcagc cgtatatggt ggcgcgaatg accgaattac tcgagctgac
    17101 gccgcagtcg cgggtgctgg aaattggcac tggttcggga tatcaaacgg caatcctggc
    17161 gcatcttgtc cagcatgttt gctcggttga acggattaaa ggcttgcagt ggcaggcacg
    17221 tcgccgcctg aaaaatcttg atttacataa tgtttcaacc cgtcatggcg atggatggca
    17281 aggttggcag gcacgtgcgc cgtttgacgc tatcattgtt acggcggcac cgccggaaat
    17341 tccaactgcg ctaatgacgc agctggacga aggcgggatt ctcgtcttac ccgtagggga
    17401 ggagcaccag tatttgaaac gggtgcgccg tcggggaggc gaatttatta tcgataccgt
    17461 ggaggccgtg cgctttgtcc ctttagtgaa gggtgagctg gcttaaaacg tgaggaaata
    17521 cctggatttt tcctggttat tttgccgtag gtcagcgtat cgtgaacatc ttttccagtg
    17581 ttcagtaggg tgccttgcac ggtaattatg tcactggtta ttaaccaatt tttcctgggg
    17641 gataaatgag cgcgggaagc ccaaaattca ccgttcgccg cattgcggct ttgtcactgg
    17701 tttcgctatg gctggcaggc tgttctgaca cttcaaatcc accggcaccg gtcagctccg
    17761 ttaatggcaa tgcgcctgca aatactaatt ctggtatgtt gattacgccg ccgccgaaaa
    17821 tggggacgac gtctacagcg cagcaaccgc aaattcagcc ggtacagcag ccacaaattc
    17881 aggctactca acaaccgcaa atccagccag tgcagccagt agctcagcag ccggtacaga
    17941 tggaaaacgg acgcatcgtc tataaccgtc agtatgggaa cattccgaaa ggcagttata
    18001 gcggcagtac ctataccgtg aaaaaaggcg acacactttt ctatatcgcc tggattactg
    18061 gcaacgattt ccgtgacctt gctcagcgca acaatattca ggcaccatac gcgctgaacg
    18121 ttggtcagac cttgcaggtg ggtaatgctt ccggtacgcc aatcactggc ggaaatgcca
    18181 ttacccaggc cgacgcagca gagcaaggag ttgtgatcaa gcctgcacaa aattccaccg
    18241 ttgctgttgc gtcgcaaccg acaattacgt attctgagtc ttcgggtgaa cagagtgcta
    18301 acaaaatgtt gccgaacaac aagccaactg cgaccacggt cacagcgcct gtaacggtac
    18361 caacagcaag cacaaccgag ccgactgtca gcagtacatc aaccagtacg cctatctcca
    18421 cctggcgctg gccgactgag ggcaaagtga tcgaaacctt tggcgcttct gaggggggca
    18481 acaaggggat tgatatcgca ggcagcaaag gacaggcaat tatcgcgacc gcagatggcc
    18541 gcgttgttta tgctggtaac gcgctgcgcg gctacggtaa tctgattatc atcaaacata
    18601 atgatgatta cctgagtgcc tacgcccata acgacacaat gctggtccgg gaacaacaag
    18661 aagtgaaggc ggggcaaaaa atagcaacca tgggtagcac cggaaccagt tcaacacgct
    18721 tgcattttga aattcgttac aaggggaaat ccgtaaaccc gctgcgttat ttgccgcagc
    18781 gataaatcgg cggaaccagg cttttgcttg aatgttccgt caagggatca cgggtaggag
    18841 ccaccttatg agtcagaata cgctgaaagt tcatgattta aatgaagatg cggaatttga
    18901 tgagaacgga gttgaggttt ttgacgaaaa ggccttagta gaagaggaac ccagtgataa
    18961 cgatttggcc gaagaggaac tgttatcgca gggagccaca cagcgtgtgt tggacgcgac
    19021 tcagctttac cttggtgaga ttggttattc accactgtta acggccgaag aagaagttta
    19081 ttttgcgcgt cgcgcactgc gtggagatgt cgcctctcgc cgccggatga tcgagagtaa
    19141 cttgcgtctg gtggtaaaaa ttgcccgccg ttatggcaat cgtggtctgg cgttgctgga
    19201 ccttatcgaa gagggcaact tggggctgat ccgcgcggta gagaagtttg acccggaacg
    19261 tggtttccgc ttctcaacat acgcaacctg gtggattcgc cagacgattg aacgggcgat
    19321 tatgaaccaa acccgtacta ttcgtttgcc gattcacatc gtaaaggagc tgaacgttta
    19381 cctgcgaacc gcacgtgagt tgtcccataa gctggaccat gaaccaagtg cggaagagat
    19441 cgcagagcaa ctggataagc cagttgatga cgtcagctgt atgcttcgtc ttaacgagcg
    19501 cattacctcg gtagacaccc cgctgggtgg tgattccgaa aaagcgttgc tggacatcct
    19561 ggccgatgaa aaagagaacg gtccggaaga taccacgcaa gatgacgata tgaagcagag
    19621 catcgtcaaa tggctgttcg agctgaacgc caaacagcgt gaagtactgg cacgtcgatt
    19681 cggtttgctg gggtacgaag cggcaacact ggaagatgta ggtcgtgaaa ttggcctcac
    19741 ccgtgaacgt gttcgccaga ttcaggttga aggcctgcgc cgtttgcgcg aaatcctgca
    19801 aacgcagggg ctgaatatcg aagcgctgtt ccgcgagtaa gtaagcatct gtcagaaagg
    19861 ccagtctcaa gcgaggctgg cctttttctt ttgtttggga agcttgtgtt tttgtcgcct
    19921 ggataagaca cgtcagcgtc ctacccggca atcagcattc tatgtgctca tcatcttgcg
    19981 caccaactgc ataaacagct cctgctcttc tccactcaaa cgccccaaaa attcctcatc
    20041 cacgctgtca ccaataggta tcgccgcagc aagtagcttt tctccttcgg cagtcagcca
    20101 gacaaagcgt cgccgcttgt cagcgacgtc atgttcacgt ctgactaagc cacgattttc
    20161 cattcttgcc aacatttctg ctagcgttgc tttggtgctg actgctgctt ctatcagtgc
    20221 cacctgttca ataccaggtt tatcagcaat ggcgcgcatc accgcgtact gtggtttggt
    20281 caggtcaggt aactcgtgct gccagcgagc cgtgtgctgc tgaaaaagct gtcgtaactg
    20341 atggaacgct ttatttcgta acgccatgta aactccgggc taattctctg tcgctatcat
    20401 agcggttttt tagtgacgaa gaagatgttg ttacttttca atccttgccg ctgtggaaat
    20461 gaaggagtac gttaataata acgttcgtat acgaacaatt aagagaataa gcaatgaaac
    20521 tgatcgtcgg gatgacaggg gctaccggtg cgcctcttgg tgtggcatta ctgcaagcgc
    20581 tgcgtgagat gccgaatgtc gagactcatc tggtgatgtc gaagtgggcg aaaaccacca
    20641 ttgaactgga aacgccttac agcgctcgcg atgttgctgc cctcgcagac ttcagccata
    20701 acccggcgga tcaggcggcg atcatctcat ccggttcttt tcgtaccgac ggcatgatcg
    20761 ttattccgtg cagtatgaaa acgctcgccg gtatccgcgc tggttacgcc gatggcctgg
    20821 tagggcgcgc ggcggacgtc gtgctcaaag aaggccgcaa actggtgctg gtgccgcgtg
    20881 aaatgccgct tagcaccatc catctcgaaa atatgctcgc actttcacgc atgggcgtgg
    20941 cgatggtgcc gccgatgcct gccttttata accatcccga aacggtagat gacattgtcc
    21001 accatgtggt agcccgcgtg ctggatcaat ttggcctcga acatccccac gccaggcgct
    21061 ggcaaggatt gccgcaggcc cggaattttt ctcaggagaa tgaataatgg catttgatga
    21121 tttacgcagc tttttacagg cgcttgatga ccacggccag ttactgaaaa tcagcgaaga
    21181 agtgaacgct gagccggatc tggcagcagc agctaacgcc accgggcgta tcggcgacgg
    21241 cgcgcccgcg ctgtggtttg ataatattcg cggctttacc gatgcccgcg tggcgatgaa
    21301 taccatcggt tcctggcaga accacgcgat ttcccttggc ctgccaccaa acaccccggt
    21361 caaaaagcag attgatgagt ttatccgccg ctgggataac ttcccgattg ccccggagcg
    21421 ccgcgccaat ccagcctggg cgcagaacac cgtcgatggc gaagagatca acctgttcga
    21481 tattctgccg ctgtttcgcc tgaatgatgg cgatggcggt ttctatctcg acaaagcgtg
    21541 cgtggtttcc cgcgatccgc tcgacccgga taacttcggc aagcagaacg tcggcatcta
    21601 ccgcatggaa gtgaagggca agcgtaagct cggcctgcaa ccggtgccga tgcacgatat
    21661 cgccctgcat ctgcataaag cagaagagcg cggtgaagat ctgccgattg cgatcacgct
    21721 cggtaacgat ccgatcatca cgctgatggg ggccacgccg ctgaaatacg atcagtctga
    21781 gtatgaaatg gcaggcgcgc tgcgtgaaag cccgtacccg atcgccaccg ccccgctgac
    21841 cggttttgat gtgccgtggg gttcagaagt gatcctcgaa ggggttatcg aaagccgtaa
    21901 acgcgaaatc gaagggccgt tcggtgagtt taccgggcac tactccggtg ggcgcaacat
    21961 gaccgtggtg cgcatcgata aagtctctta tcgcagcaaa ccgattttcg aatcactcta
    22021 tctcggtatg ccgtggaccg aaatcgacta cctgatgggg ccagccactt gcgtaccgct
    22081 gtatcaacaa ctgaaagccg agttcccgga agtgcaggcg gtaaatgcca tgtacaccca
    22141 cggcctgctg gcgattatct ccaccaaaaa acgctacggc ggctttgccc gcgcggtggg
    22201 cctgcgtgcg atgaccacgc cgcacggtct gggctacgta aagatggtga ttatggtcga
    22261 tgaagacgtt gacccgttca acctgccgca ggtgatgtgg gcgctctcct cgaaagtaaa
    22321 cccggcaggg gatttggtgc agttgccgaa tatgtccgtg ctggaactcg accctggctc
    22381 aagcccggcg gggatcaccg acaagctgat tatcgacgct accacgcctg tcgccccgga
    22441 caaccgtggt cactacagcc agccggtggt ggatttgccg gaaaccaaag cctgggctga
    22501 aaaactgacc actatgctgg ctgcacgtaa ataaggagaa caagatgatt tgtccacgtt
    22561 gtgccgataa acagattgaa gtgatggtga aatcgccggt gaaagatgtc tggacggtat
    22621 atcagtgcca gcattgcctt tatacctggc gcgataccga accgctacgc cgcaccagtc
    22681 gcgaacatta cccggaagcg ttccgcatga cgcagaaaga tattgatgat gcgccaatgg
    22741 tgccgagcat cccgccgctg ctggcggaag gtaagcgcta aataaaaagg tccgatgccc
    22801 atcggacctt tttattaagg tcaaattacc gcccatacgc accaggtaat taagaatccg
    22861 gtaaaaccga gaatggtcgt taacactgtc caggttttca gaccgtctgc taccgacaac
    22921 cccagatatt tggtcacaat ccagaaccct gagtcattaa tatgtgacgc cccaagccca
    22981 ccaaagcagg ctgccagcgt caccaatacg cactgaatcg gattcaatcc catcaccgct
    23041 tctgagagta acccgccggt tgtcagtatt gctacggttg ctgacccctg cgatgcacgc
    23101 agcgccagtg aaataataaa tgcggctggt aacagaggca ggtcaatcat ttgtaacatg
    23161 ttggcaaggg ctttgccgac gcccgattcc accagcactt tgccaaatac ccctccagca
    23221 ccagtaacca aaatcactac cgccgcagta ggaagcgctg agcccataat gtcgccggtg
    23281 tgttgtaagc tccagccgcg acgtaaagcc aataaccaga atgccagcac cagcgcaatc
    23341 attagagcta ccattggtga gccgatcagc tgtagcgtac caagcagggg atgcgaaggc
    23401 ggcatcagtg ttgcggaaac cgtacccgcc atgataatcg cgataggaat aacaattagc
    23461 gaggtgacca gcgcgacgcc cggtggattt attttatcgc ttaattttgt cgcgccttcc
    23521 tcactggccg gagccagttg catctgttcc agtacttcta ctgacatcgc atattggcgt
    23581 ttattgatta ttttcgctgc aaagtagcca acaaccccta cgggaataga aatcgcaata
    23641 ccgatgatgg ttagccagcc gatgtctgcg tggagtaacc ccgctgcggc gacagggcct
    23701 ggatgcggcg gtaccgccac gtgaacagtg agcatgatcc cagcgacagg caggccaaat
    23761 ttgagtggcg atattttggc taccttggca aaaccgtaaa tgattggcgc aagaataata
    23821 aagccgacat caaagaagac gggaataccg aggaagaacg ctgccagagt cagcgcagcg
    23881 atagttcgtt tgtcacctaa cttgcgactg aaataattag ccagtgactc tgcaccacaa
    23941 gagtgttcga tcatacgccc cagcatagcg cccagaccaa taataatagt gacggaacca
    24001 agcacaccgc ccatcccggc gatcatcact ttacccactt cgcccgccgg tatacccgcc
    24061 gcaagtgcga ctaacaggct gacgaggagc agagcaacga atggttgtac ctttgccttg
    24121 atgaccagca gcaacagcat gattacgcca gctaacgcaa tgcataacaa tgtaattgtg
    24181 gacatgggaa accctgtctg aaagttatag ttaacctacc ccatccgtag atggggggat
    24241 gtatgggtac gttgtaatta gggatttaac gaattagcgc taggcgtcaa accagccaag
    24301 cccttcttcg gtgaggccac gaggtttata ttcacaaccg atccagccct gatatcccac
    24361 ctcatcgaac aggcggaaca gccacggata gttgatttct ccatcgtccg gttcatgtcg
    24421 atcaggtagt ccggcaattt gtacgtgcgc atatttcccg gcgtagtcgc ggattaaatg
    24481 cgtcaggttg ccatctactt tttgcgcatg aaaagtatct agttgaataa acacgttatc
    24541 tcgcgcaacc tcttcaacaa tagccagtgc ctgatactgg ctggagaaga gataatgagg
    24601 cttaacgccg gggctgagtg cttcaactaa tattcgcttg ccgtgtggcg caaagcggtc
    24661 ggcagcgtag cggagattat cgataaatac tgcccggtac cgttcagcat cttcgccagc
    24721 gggcacgacg cctgccatca catggacttg ttcacaattg agcgccaatg catattccag
    24781 tgccaggtcg atgtctgcgt gtgcttcgtg ctcacgtccg ggaagggcgg ataatcccca
    24841 ttcccccgca ttaatatctc cgggagcggt attgaacagc gccagtgtca gatggttttg
    24901 ctccagttgc ttttggattt gcagggtgga gtagttatag ggaaacagaa attccacagc
    24961 atcgaacccg gcttttcgcg ctgcggcgaa gcgttcaata aaaggcactt cggtgaacat
    25021 catggataaa ttagctgcaa aacgaggcat tgcattaact ccttaattcc gcaatttcac
    25081 ctgcggtcag ataacggatc gggcggtcac cgagaataaa aatcagcttt gccgtttcct
    25141 ccagctcttc catattgttg gcggcttctt gcaggctttc accgcaaacc actgggccat
    25201 gatttgccag taaaaaagcc tgattgtctg ctgccagttc cgccagatcc tgtgcgatgc
    25261 gtttatcgcc cggtcggtaa tagggcacca gcgggacatt tcccatccgc atcaccacgt
    25321 atggtgtgaa cggacgaata acgttgctgc tgtccagccc ttgcaggcag gaaagcgccg
    25381 tcgaccatgt gctgtgcaaa tgcaccaccg ctttacagcg cggattgttg cgatacagcg
    25441 ccagatgaaa gagcacctct ttcgagggtt tgtcaccact taaccattcg ccatccgcgg
    25501 cgactttgga aagccgctgc ggatcgagat tgcccaggca tgaacctgtc ggtgtcgcca
    25561 gtaaattccc gtcaggtaaa agcagcgaca gattgccagc cgaaccggtt gcatagccgc
    25621 gctgaaagaa tgaactggca atccgcgtca tctcctctcg caaagactgc tctacttttg
    25681 cgaaatcgct catgataaaa actctctttg ggctcgtgaa aaaaaggctt catcaccgaa
    25741 gttgccagat ttaagggcga gtgagacagg cttatccagt gcgtttaccc acggcacgcc
    25801 gggggaaatg gttgggccaa tatgaaaccc ttttatgccc aggctctgtg tgactacgcc
    25861 ggaggtctca ccgcctgcga caataaagcg tgtcacgcct tccgctgcta accgcgccgc
    25921 tagttgagaa aacagagttt ctactgcctg actggctttt tgtgcaccgt attgctgttg
    25981 aattgctgcc aatgcgtcag tgctggcggt ggcaaaaacc agtggagcaa gtacactttc
    26041 ctggcccaga acccactctg ccagttcgtg tgcataagcg gccagagttt cggttgagag
    26101 gcagtgtgcc acatcaactt cacgggctgg tgcaatttga cggtaatgtg ccacctggcg
    26161 gttggtcatt tgagagcatg aaccggagag cactacgccg cgcccagcga gcggacgccc
    26221 tgcttcgcga gcctggttac cgttttcttg cgcccactgc cgggccaggc caatcgccag
    26281 accagaaccg cccgttacca gtggggcatc gcgcaaggct tctccctgaa tttccagatg
    26341 gtgttcggtc agcgcatcaa gcaccgcgta gcggtagccc tcttgctgta agcgagccag
    26401 ctcttgacga acggcatcca caccttgttc gaaaacatgt gccgaaacga cgccgcagcg
    26461 ccctgtggat tgcgcttcaa ccagacgggg aagatagctg tcggtcatgg gatttaccgg
    26521 gtgatggtgc atcccggatt cggccagcag ttgattcatt acgaacaaat acccctgata
    26581 aaccgtacgt ccgttgaccg gcagggccgg agagaagacc gtaaacggcg tgtcgagagc
    26641 atccattaat gcatcggtaa ccgggccaat attacctttc gccgtactgt cgaaagtaga
    26701 gcagtatttg aaatagatct gtttgcaacc ttgctgttgc aaccagctca gagccgccag
    26761 cgattgctgt gtggcttcaa ccaccggaca ggagcgcgtt ttcaggctga tcaccagtgc
    26821 gtcgattgct tccggcattt tacctgttgg aacaccgtta atttgtaccg ttggtagacc
    26881 gttttccacc agaaaactgg cgatatccgt cgcgccggta aaatcatcgg cgataacgcc
    26941 aatcttgatc atgatttcgc tcccggtaga gtgatgccag agaaaatctt gataactgcg
    27001 ctatcgtctt ctttcccgta acccgcgtta ctggcgctgg tgaacatatt caatgctgtt
    27061 gaggccaatg gcagcgggaa gtgcagggct ttggctgtat cggtaaccag accaagatcc
    27121 ttaacaaaaa tatcgacggc tgaatgcggg gtgtaatcgc catccaccac atgacgcatc
    27181 cggttttcga acatccagga atttccggcg gcattggtca cgacgtcata catcacatcc
    27241 agcgggatcc ccgcacgggt gccagtcgtt caaaggcaat atcgctaccg gaggccatga
    27301 cagtcatttc accgttagcg gcttttactg caccaccaga aactggcgca tccagcattt
    27361 ccagatcgaa tccagccaga gcggtagcaa tttcttgcgc atcagcacta gcgatagtgg
    27421 aagaaaccat tactgccgta ccgggtttca gatgttgtgc aacgcctgtt tcaccaaaca
    27481 gcacctgttt aacctgggcc gcattgacca ccagcaccag cagagcgtcc agtttttcgg
    27541 caaacgtcgc ggcgttatca gaaaccccgc aagcacctgc ctctttcaac gtagcgcagg
    27601 cattgctgtt caggtctgcg ccccaggtag aaaaacctgc gcggacacat gacagtgctg
    27661 ctcccattcc cattgaccct aagccaacga taccgacatg aaactcagat cccgttttca
    27721 tctgctctta ttgttaattt aagtgatatt ttgtttgata ttgtgaatat gagcgctgaa
    27781 agataacgat atggtgagct gattcacata aattaacatt ttgtgttatt ttatgtgaac
    27841 taagcgttag ttgcgccgcg cacgtttcgc aggcaaatag cgtagaatgt cagcaggaca
    27901 aaggaaggag caaaagttga tacccgtaga gcgtcgccaa atcatccttg agatggtagc
    27961 tgaaaaaggc attgtcagta ttgctgaact aacggacaga attaatgtgt cacatatgac
    28021 cattcgtcgg gatttacaaa aactggagca gcagggagcc gttgtgctgg tgtccggagg
    28081 cgtccagtct ccgggacgcg tggcgcatga accttctcat caggtaaaaa ctgcgctggc
    28141 aatgacgcaa aaagcggcta ttggcaagct ggcggcaagt cttgttcagc cgggaagttg
    28201 tatctatctg gatgcgggaa cgaccacgtt agcgatagca cagcatctga ttcacatgga
    28261 gtcactgact gtggtcacaa acgatttcgt tattgcgaac tacttgctcg acaacagtaa
    28321 ttgcacaatt attcacactg gcggtgcagt gtgtcgggaa aaccgttcct gtgtcgggga
    28381 agccgctgcg accatgctgc gcagcctgat gattgatcag gcttttattt ctgcatcgtc
    28441 atggagtgtg cgggggattt ctacgccagc ggaagataaa gtcacggtga aacgggcgat
    28501 tgccagtgcc agccgccagc gagttttggt ctgtgatgcg acgaaatatg gtcaggtggc
    28561 gacatggctg gcgttaccat taagcgagtt tgatcagatt attacagacg acggtttgcc
    28621 ggagagtgcc agtcgcgcgc tggcgaagca ggatctctct ttgctggtag cgaaaaatga
    28681 ataatggcct gcaataacat ctggttactc atgcttcaca gaagaagcat gagactacct
    28741 tattttataa aatgacagcc gcccactttt cggcgatccg gtatcaatat aaatctggtt
    28801 agcgaacgtc tgaatgttat caaacatcat atgtccaaat ataaaataat cagcgccgtt
    28861 tatttgttgt aactcgccat taagcgattt ctgcatacga taaacaggac agagtaattc
    28921 gctctccgct atttctttgc caaagagata ttcatcaccc ggataatctg catgtgcgat
    28981 gacatatttt attatgtcgt tagtgatttc aataatatgt ggaaggtgat ggaatttcag
    29041 caacagatct attgcctctt gttgctctga atcatttaaa tcgaaaaacc agtcaccacc
    29101 gctggcaagc cacatattgc catcgccggt cgcgaacgca tccagcgcca ttgcttcgtg
    29161 gttgccttta acggagataa accagggctg gtttagcagg cgcaggacgt caagactctc
    29221 cggcccacga tcaatattat cgccgacaga aataagtaag tcggtttcgg ggaaaaaaga
    29281 gagttgatgt aagcgggatt gtaataactg atattcacca tgaatatcac caacgaccca
    29341 tatatggcga tagtgatggg cattgatttt ttgatagcgt gtagatggca tggttttacc
    29401 ctgtaaaata agctttccta ttatacaggg tatttttatt tgattcgtca gttgtcgtta
    29461 atattcccga tagcaaaaga ctatcgggaa ttgttattac accagactct tcaagcgata
    29521 aatccactcc agcgcctgac gcggagtcag tgaatccggg tcgagatttt ccagtgcttc
    29581 gaccgcaggc gaagtttctt ctggcactga cagcaaagac atctgcgtac catccacttg
    29641 cgtcgcggcg gcgttcggcg aaatgctttc cagctcacgc agtttttgcc gtgcgcgctt
    29701 aataacctct ttcggtacgc ccgccagagc agcaaccgcc agaccgtagc ttttgctcgc
    29761 cgcgccgtcc tgcacgctgt gcataaaggc gatagtgtcg ccgtgctcca gcgcatcgag
    29821 atgtacgttg gcgacgcctt ccattttctc cggtagctgg gtcagctcga aatagtgggt
    29881 ggcaaacagc gtcaatgcct taatcttatt cgccagattt tctgcgcacg cccacgccag
    29941 cgacagacca tcgtaggtgg acgttccacg cccgatctca tccattaaca ccagactgta
    30001 ttcggtggcg ttatgtaaaa tattggcggt ttcagtcatc tccaccataa aggttgagcg
    30061 cccggaagcc aggtcatctg ccgcgcctac gcgggtaaag atgcgatcga taggtccaat
    30121 ctcgactttt tgtgccggta catagctgcc gatgtaggcc atcagcgcaa tcagtgcggt
    30181 ctggcgcata taggtacttt taccgcccat gttcgggccg gtaataatca acatacggcg
    30241 ctgcggcgac agattcagcg ggttagcgat aaatggccca ttcagtactt gttcaactac
    30301 cggatggcga ccttcggtaa tgcgaatgcc cggtttatca atgaaggtcg ggcaggtgta
    30361 gttcagggta taggcccgtt ccgccaggtt aaccagcacg tcgagttccg ccagcgcgct
    30421 cgcgctctgt tgcaacgctt ccagatgcgg caacagcagg tcgaacagct cttcataaag
    30481 ctgtttttcc agtgccagtg ctttgccttt tgaggtgaga actttatctt cgtactcttt
    30541 tagctctgga atgatgtagc gctcggcgtt tttcagcgtc tggcgacgca tgtagttgat
    30601 gggtgccaga tggctttgcc cacggctgat ttgaatgtag tagccgtgca ccgcattaaa
    30661 gccaactttc agcgtgtcca ggccggtacg ttcacgctcg cggacttcca gacgctccag
    30721 ataatcggtc gcgccgtcag ccagcgcgcg ccactcatcc agctcttcgt tatagcccga
    30781 tgcgataaca ccaccgtcgc gtaccagcac cggcggtgtg tcgatgattg ctcgctccag
    30841 cagatcgcgc agctcggcaa actcgcccat cttctcacgt agcgcctgta ccggtgcact
    30901 atcgacagtt tctaactgcg cacgcagctc cggcagttgc tggaaagcgt ggcgcatacg
    30961 ggccagatcg cgtgggcgag cagttcgtaa agccagacgt gccagaatac gttccaggtc
    31021 gccgacctga cgcagtaccg gctgcaactc ggcggtgaaa tcctgcaatg cgccaatagt
    31081 ttgctggcgc tcaagcaaca cgcgggtatc gcgcactggc atatgcagcc agcgtttcag
    31141 catacgacta cccatcggcg tgacggtgca gtcgagcaca gaagccagcg tattttccgc
    31201 accgcccgcc aggttctgag taatttccag gttacgacgt gtcgcggcat ccataatgat
    31261 gctgtcctgc tcacgttcca tggtgataga acgaatatgc ggcagggtcg tgcgttgggt
    31321 atctttcgca tactgcaaca gacaaccggc agcacaaagt ccgcgcggcg cgttctcgac
    31381 gccaaaaccg accagatcgc gggtgccaaa ttgcagattc aactgctggc gcgcggtgtc
    31441 gatttcaaac tcccacagcg ggcgacggcg caggccgcga cggccttcaa tcagcgacat
    31501 ctcggcgaaa tcttctgcat acagcagttc cgccggatta gtgcgttgca gctctgccgc
    31561 catcgtttcg cggtcggccg gttcgctcag gcgaaaacgc ccggagctga tatccagcgt
    31621 cgcgtagccg aaacctttgc tgtcctgcca gatagccgcc agcaggttgt cctgacgctc
    31681 ctgtaacagg gcttcatcgc tgatggtgcc tggcgtaacg atacgcacaa ctttgcgctc
    31741 aaccggacct ttgctggtcg ccggatcgcc aatttgttcg cagatggcaa cggactctcc
    31801 ctgattcacc agtttggcga gatagttttc caccgcatgg tagggaatcc ccgccatcgg
    31861 gatcggctct cccgccgaag caccgcgttt ggtcagtgaa atatccagca gttgcgacgc
    31921 gcgttttgcg tcgtcataaa acagttcata aaaatcaccc atccggtaaa acagcaggat
    31981 ctcgggatgc tgggctttca gcttgagata ctgctgcatc atgggcgtat gggcgtcgaa
    32041 attttctatt gcactcatgg ggttatgtcc ggttccctga tattaaatgg ggtgtgatgg
    32101 tttttatttt cgttgtaatc acataaggcg cacatgatac cggagttaat catttcaggg
    32161 gaagaagcga acggcgagtt cttgatgctg gttccgggaa agatgctgtt ttgcagcttt
    32221 tgcgtgatgc ttatgaagca agtacgcaaa attgtatcga ttggcgaacc cttcttcttt
    32281 agagtcatag tgccctttaa gattaagatg taatataaat gcatcttaat gaggtggagg
    32341 cagcatgtcc ggcaagcgta tctctcgtga aaaactgacg attaaaaaaa tgatcgatct
    32401 ttatcaagcg aaatgcccgc aggcgtcagc ggagccggag cattacgagg cgttgtttgt
    32461 ttacgcgcaa aagcggctgg ataaatgtgt gttcggcgaa gagaaaccag cctgtaagca
    32521 gtgtccggtg cactgttacc agcctgcaaa gcgtgaagag atgaaacaga ttatgcgttg
    32581 ggccgggccg agaatgctgt ggcgtcatcc gatcttaacc gtgcgtcatc tcatcgatga
    32641 caagcgtccg gtgccagaac tgccggaaaa atatcgtccg aaaaaacctc gtgaatagta
    32701 tgccggacag gcagatctgt ccggcaattt gcagttaaat caatgccgat ttatcaattc
    32761 ccagccgctt catccgtgac agcagggtcg tgcgtttcag ccccagacgt tgcgcagcgc
    32821 ctttaggccc ggcaaccacg ctgttggttt ctttcagcac gcgcacaatc aactgatatt
    32881 catcttcgcc ctcctgggcg acaaccgttg cggcaggcgg cgtttcaggc tccggtaaag
    32941 caatatctgg caatgacagc tgcagcacgt tgccgcgtgt tagcaatact gcgcgctcaa
    33001 tgacgttttc cagttcgcgt acgttacccg gccactccat gttgctcaag gtgcgcagcg
    33061 tctcggcagg aatgctgtcg atattgcgcc ccagacgacg ggcaattttg aaggtaaagg
    33121 ctttcgccag cagcggaata tcttccggac gctcgcgtag tggcggcagg tgaatcggga
    33181 atacgttcag gcggtaatag agatcgctac ggaactctcg gtcggcgacc atttttttca
    33241 gatcgcggtt agtcgcggca attaaacgta cgtccgtctg aatgattttg ttgctgccga
    33301 gacgttcaaa ctcctgttcc tgcaatacac gcagcaactt cggctgtaac tccagtggca
    33361 tatcgcccac ttcgtcgagg aacagagagc ttttatccgc cagttcaaaa cgaccaatgc
    33421 gctgggcgct ggcaccggta aaagccccac gctcatgacc aaacagatcg ctttccagca
    33481 atccggcagg catcgccgcg cagttcattt tgaccatgcg gcgattatta cgcccactga
    33541 gattatggat cgcacgggca atcagctctt taccagtgcc agtttcaccg aggatcagca
    33601 cggtactgtc actttgcgcc accatttcaa cttgtttaag cacgctgtac atggcttcgc
    33661 tgcggccaat aatctcgcca aattcactat caacattgtt gagctgctcg gtcagggcga
    33721 ggttttcatc aaccagccgt tctttcagac gatggatttc ctgataggcg agggcgttat
    33781 cgacagcgat tgccacacgt tcggcaatct ggcgcagcaa attcagattg gtagtggtaa
    33841 acactttctc ttcgcattgc gccagtttca gcacgcccag catggtgtcg ccagacatca
    33901 gcggtaacag gcacaaggtt tgaatctggt tgccccaggt gtcgaacaac atgcgttcat
    33961 agggggctaa atcgtcccgc tcgtggagat tgatcagcag catctcttta cttttgaaca
    34021 cgcgttcggt gagggttccg gcttcatcga cttcgctctg ttcgtgggcg ggatgttgtt
    34081 tatcaagata gtgagtggag tagatgttga gtttgttttt acggtggctg cgtaagacaa
    34141 tgctgatatc gtcaatgtcg aaatagtaat ggatttcttt ggcgacttcg ctgaccagtt
    34201 cgtccatatc caggcgggaa agcaccgcgt tggtaatggc aaccaggatg cggaagttat
    34261 cgagctcccg gcataacaac tcatagtcga cattgttgac gacgcggctc tggatttgtt
    34321 cggtgacgac agaaacgatc tgcgtaaatg tttgcagacg attgaactct ttttcgctcc
    34381 agggacgatc gtcataacga ataaattcac agccaccaaa aatatgccct tccgccgcca
    34441 gtggcatcag gcaatagtga ccaaatttgg ggtatagccc acctgcggcc aggtttcaca
    34501 aaattcttcg taactgcaat gcagcgtatc agggcgcgac aaaatgctgc gtaccggacc
    34561 gtgtgccaga acagtttcgt cttcatattt aatgggggtg tctttttcac gcgacgcgta
    34621 ataagacgca cgttgagtct gcgcttgcca caacacaata gccgcgttgt cggcgagcgc
    34681 agaacgcttt accagttgcg aaagagcctc acacagcgag gccagatcgg gctgctgcaa
    34741 tagtgtccga gtgatgtcga acaacccttg ctgtccgaga tcactcatcg gtgtatatga
    34801 cattttgttc ttccaggaaa gcaccgcaaa gcggtgcaaa aggtcattga ctaactatag
    34861 gagatttaga attttattag catatacgcg gaagcggttc ggcgtgtggt aaatcgaggg
    34921 ttcgtttcac gccatacaga ccggcaagac gaacaccttt acgttccacc acttcaccaa
    34981 tcagcgccgc gtctttcccc agtggatggg aatgtaacgc tgccagcact tgctctgccg
    35041 cgttgcgttc aacggcgatc accagtttgc cttcgttggc aaagttcagg gcgtccagtc
    35101 ccagcaattc gcaaacgccg cgcacggcag gtttaaccgg cagcgctgat tcagaaattt
    35161 caataccaca accgcaggct gccgcgaact catgaaccac cgcgtttaca ccaccacggg
    35221 tggcatcacg cagcgctttc acgccgggaa tgtcacgcag cgtctgaata agcggcgtca
    35281 gcaccgcgca gtcgctgacc agttcgccat ccagccccag ctgctcacgc aggttaagga
    35341 tagtcgcccc gtggtcgccg agtgtaccgc taaccagcaa tacatcgcct gcggttagcg
    35401 tctgtgcgcc ccagtgaata ttcgccggaa ttgcgcccat gccagcggtg ttgataaaca
    35461 gtttatctgc cgcgccgcgc tgcaccactt tagtatcgcc agtaacgatg gcaatgcctg
    35521 ccgtgcgggc ggtttctgcc atgctggtca ctacggcttt cagtgtctcc atcggcaatc
    35581 cttcttcgag gataaagcca caggagagat agcgcggaat agcgccactg accgcaacgt
    35641 cattcgcggt gccgcaaatc gccagcttgc cgatattacc gccaggaaag aacagcgggt
    35701 caatgacgta actgtcggtg gagaacgcca gacggtcgcc ttccgctacc agctgcgcca
    35761 gatcaagacg tgcctgatct tcctgctctg ccagccacgg gttggcaaag gcttccataa
    35821 acaggctgtt gattaattgc tgcatcgcct ggccgccgct gccgtgggcg agttggatat
    35881 tattcacgct tcactctcct gctgacgata ctgataccac gcggcgcacg ctccttcgga
    35941 ggagaccatc agtgcaccaa atgcggtttg aggattacag gtgttaccaa acagcgggca
    36001 ttgatgcggc ttacatttgc ccgttaatac ctcaccacaa cgcgcgcgcg ggtcatcgca
    36061 gacctgctgc ggtgccgggc ggaaatgtgc ttcggcatcg aatcgttgat aatccggcgt
    36121 caggtgcacg ccagaagatt caatcacgcc taagccgcgc cattcgctgt cgccgttgac
    36181 acagaacaca tcggcaatcg cctgttgcgc cagcaggtta ccggcatccg gtaccacccg
    36241 acgatactga ttctctacct tgctgtgggc cgctattttc tgctccacca gcatgaccac
    36301 gccttgcagt agatcaaggg gttcgaaacc agcgaccacc agcggacgat gaaaatcgct
    36361 ggcgataaaa ttataggcat cagtgccgat aaccatacta acgtggcccg gcgcgaggaa
    36421 cgcgtcgata ccgttatccg gctgttccag caaactgcgc agcgtcggga taagcgtaat
    36481 gtgctggcag aagaagtaaa aattctgcac atcacgcgct ttcgcctgtt gcagagtgat
    36541 ggcggtggtc ggcatggtgg tttcaaaacc taagccgaag aacaccactt tgcgggttgg
    36601 attctcctgc gccagtttca acgcatccat cggcgagtaa acgatgcgca catcggcacc
    36661 gcgtgctttt gcctgcaaca gcgatccctg tttccctggt acgcgcatgg cgtcgccaaa
    36721 ggtacagaag atgacttccg gatggctggc aatctccacg caggtgtcga ttctacccat
    36781 cggcagtacg cacaccgggc aacccggacc gtggataaac tcaacgtttt caggcagtaa
    36841 ctggtcgagg ccgaatttaa aaatggcgtg ggtatgaccg ccacacactt ccataatccg
    36901 cagagggcgt tcggcggtgt aagagagatg tgaagcacgt tcgcgcagat gctcaattaa
    36961 ctgcatcacc tgttccggtg cgcgatattc atcaacaaaa cgcattattt ttcctcgcca
    37021 tacaacagcg cgccgacatc cggctcaacg tcaaacatgt tttgtaaggc gtcgagagtg
    37081 tcgcgtgctt cggcttcatt aattacgctc atggcaaagc caacgtgaac cagtacccac
    37141 tggcccacgc gcggctgacc gttttcatcg cagctgccga ctaacgttaa atcgacatcg
    37201 cgctgaatgc cgcagacgtc gactttcgcc tggttgccgt caatggtgcg gatctggccg
    37261 ggaacgccta tgcacatcgc tgtgtctcca gccagttcag ccactggtcc atcccttcgc
    37321 cgctggtggc ggaaataagg atgatttcaa tttctggatt gacttcgcgg gcgcaggcga
    37381 tgcacttctc aacgtcaaag ttgagatacg gcaacaggtc aactttgttg agcagcatca
    37441 gcgaggcggc ggcaaacata tgccgatatt tcagcggttt gtcttcacct tcggtaacgg
    37501 aaagcaccgc tactttgtgt ttttcaccga gatcgaagct ggccgggcat acgaggttgc
    37561 caacgttttc gataaacaga ataccgttat cgtccagtgg cagacgcggt gcggcgtcgg
    37621 caatcatctg tgcgtcaaga tggcagcctt taccggtgtt cacctgaatc gctggtgtac
    37681 cggtagcgcg aatgcgtgcg gcatcgttca cggtttgctg gtcgccttca ataaccgcgc
    37741 acggaacgct gtctttcagg cgcattaagg tttccgtcag cagggtggtt ttaccggaac
    37801 cagggctgga aaccaggttg agcaccagtt gcttgcgggc agcaaagcgc gcgcggttgc
    37861 gttcagccag acggttgttt ttgtccagca cgtcaatttc gacttccagc atccgacgct
    37921 ggctcatgcc cggtgcgtga gtgcccgctt cgccatgacc gtaatgcagg tcgccttctt
    37981 cagtctggct gggggtaaat tcaggcgctt taatgccggt gattttcatc tgcgggcgtg
    38041 ccgccggggc aaatggcgcg ctacgaaacg cggaatgagg gttatgttca tcaccctcga
    38101 tatacaggtt gccttcaccg caaccgcatg ttgtacacat cgctcactcc tggtctattt
    38161 ctatccgccg aatctgtaaa ccgtcgtctg ccacaatctg tagcatgtca ccatgacact
    38221 gtggacagcg gcggacgcgc tgggtcagta gcgtcacata ctgttggcat gtttcacacc
    38281 agcattcggc ctcttgttct tcgaggtgca gtttacaacc ttccgccacg ctgccgcggc
    38341 aaaccagatc aaaacaaaag gcaagagagc tggtttcgac acaagaaaat gcgccaattt
    38401 tgagccagac cccagttacg cattttgcgc cgtgttttgc ggcctgctgt tcgatcaatt
    38461 ccagtgcccg ttggcagagg gttatttcgt gcatatcgcc tcccattaac tattgccagc
    38521 tacaagcaat aattgcgcca gtgttgatta tccctgcggt gaataatgtc gatgatgtcg
    38581 aaatgacacg tcgacacggc gacgaaattc atctttagct taaaaatctc tttaataaca
    38641 ataaattaaa ggttggcaca aaaaatgctt aaagctggca tctctgttaa acgagtaacc
    38701 tgacaatgac tatttgggaa ataagcgaga aagccgatta catcgcacag cggcatcgtc
    38761 gtctacagga ccagtggcac atctactgca attcgctggt tcaggggatc acgttatcga
    38821 aagcgcgcct gcatcacgcc atgagctgcg cgccggacaa agaactctgt ttcgtccttt
    38881 ttgaacattt tcgtatttac gtcaccctgg cggatggctt taacagccac accatcgagt
    38941 attacgtcga aacaaaagat ggcgaagaca aacagcggat tgcgcaggcg caactgagca
    39001 ttgacggcat gattgatggc aaggtcaaca tccgcgatcg cgaacaggtt ctggaacact
    39061 atctcgaaaa aatcgctggc gtttacgaca gcttatacac cgccattgaa aacaatgtgc
    39121 cggtgaattt aagccaactg gtaaagggac aaagcccggc agcatgagct gaggctttgc
    39181 ccgttttgca ggcgttacgc ctgtttgggg atgggcgtgt cgatgagtgt cgaaaatgac
    39241 atttcatcgg catgttttcg tcaaaaatga caatcacctg aggaatgcct ggtgaatcgt
    39301 tttgtaattg ccgactccac gctctgtatc ggctgccaca cctgtgaggc cgcctgttca
    39361 gagacgcatc gccagcacgg cctgcaatca atgccgcgcc tgagagtgat gctcaatgaa
    39421 aaagaatctg cgccgcagct ctgtcaccac tgtgaagatg caccctgcgc ggtggtctgc
    39481 ccggttaacg ccatcacccg cgttgatggg gccgtgcagt tgaatgaaag tctgtgcgta
    39541 agctgcaaac tgtgcggcat cgcctgcccg tttggcgcaa ttgaattttc cggcagccgt
    39601 ccgctggata ttccggcaaa cgccaatacc ccgaaagcgc caccggcacc gcctgctccg
    39661 gcgcgtgtca gcacattgct tgactgggtg ccaggtattc gcgcgatcgc cgtcaaatgt
    39721 gacctttgta gctttgatga acaaggtccg gcctgtgtgc ggatgtgccc gactaaagcc
    39781 ctgcatctgg tggataacac cgatatcgcc cgcgtcagca aacgtaagcg tgagctgacc
    39841 tttaacacgg actttggcga tctcaccttg tttcagcagg ctcaaagtgg agaggctaaa
    39901 tgagcgcaat ttccctgatc aatagcggcg tggcatggtt tgtcgccgcc gctgttctgg
    39961 catttctctt ttcttttcaa aaagcgctaa gtggctggat agccggaatt ggcggcgcgg
    40021 ttggtagtct gtatacggca gccgcgggct tcactgtact gactggcgcg gttggcgtga
    40081 gcggtgcgct gtcgctggta agctacgatg tgcaaatctc cccgcttaac gcgatttggc
    40141 tgattaccct cggcctttgc ggtctgtttg tcagcctcta taacattgac tggcatcgcc
    40201 acgcgcaggt gaagtgcaac ggcttgcaga tcaatatgtt gatggctgcc gccgtctgcg
    40261 ccgtcatcgc cagcaacctc ggcatgttcg tggtaatggc cgaagtcatg gccttgtgtg
    40321 cggtgttcct caccagcaac agcaaagagg gcaaactgtg gtttgcgctg gggcgtcttg
    40381 gcactctgct gctggtgatt gcttgctggc tgctgtggca gcgttatggc acgctggatc
    40441 tgcgcctgct ggatatgcgt atgcaacagc tgccgctcgg ttccgatatc tggctgctcg
    40501 gtgtgattgg ctttggcctg ctggctggga ttattccgct gcacggctgg gtgccgcagg
    40561 cacatgctaa cgcctctgcg ccagctgccg cgttgttttt tacggtagtc atgaaaattg
    40621 gcctgctggg catgttaacc ctgtcactgc tgggcggtaa tgcaccgctg tggtgggggc
    40681 gcgctgctgg tgctcggcat gatcaccgcg ttcgtcggtg gtctgtatgc gttgatggaa
    40741 cacaacatcc agcgcctgct ggcttaccac accctggaaa atatcggcat tatcctgctg
    40801 gggctgggcg ctggcgtaac gggtatcgcg ctcgaacaac cggcgctgat tgctcttggc
    40861 ctggtcggtg gtctgtacca tctgcttaac catagcctgt tcaaaagcgt actgttcctc
    40921 ggggcgggga gcgtctggtt ccgtaccggt catcgcgata tcgaaaaact cggtggtatt
    40981 ggcaagaaaa tgccggttat ctccatcgcc atgttagtcg ggttgatggc aatggctgcg
    41041 ctgccgccac tgaacgggtt tgccggggaa tgggttatct atcaatcctt cttcaaactg
    41101 agcaatagcg gcgcgtttgt tgcccgtctg ctggggccgc tgctcgctgt gggtctggca
    41161 attaccggtg cgctggcggt gatgtgtatg gcgaaagtct atggcgtcac gttcctcggc
    41221 gcgccgcgta ccaaagaagc cgaaaacgcc acctgtgcgc cgctcctgat gagcgtaagc
    41281 gtagtggcac tggcgatttg ctgcgtaatt ggcggtgttg ctgcgccgtg gctgctgccg
    41341 atgctctctg ctgctgtacc tctgcctctg gagcctgcta acaccaccgt ttctcaacca
    41401 atgatcacat tgctgctgat tgcctgcccg ctgctgccat tcatcattat ggcgattttc
    41461 aaaggcgacc gcctgccgtc acgttcccgc ggtgcggcct gggtgtgcgg ctacgaccat
    41521 gaaaaatcaa tggtgattac cgctcacggt tttgccatgc cggttaaaca ggcgtttgcg
    41581 ccagtgctga aactgcgcaa atggctgaat ccggtgtcgc tggtgccggg ctggcagtgc
    41641 gaaggtagtg cgttgctgtt ccgccggatg gcgctggttg aactggcggt actggtggtg
    41701 attattgttt cacgaggagc ctgagaatga gtgttttata tccgttaatt caggcgctgg
    41761 tgttatttgc cgttgcgccg ctgctctccg gtattacccg cgtggcgcgc gcccgtctgc
    41821 ataaccgtcg cgggccgggc gtgttgcagg agtatcgcga cattatcaaa ctgctgggcc
    41881 gccagagcgt tggcccggat gcctctggct gggtgttccg cctgacgccg tatgtgatgg
    41941 tgggcgtcat gctgactatc gctactgcgc tgccggtggt gaccgtcggt tctccgctgc
    42001 cgcaactggg cgatttgatc accttactgt atctcttcgc catcgcgcgt ttcttctttg
    42061 ccatttccgg tctggatacc ggtagcccgt ttaccgctat cggtgcgagc cgtgaagcga
    42121 tgcttggcgt gctggtcgaa ccgatgttgc tgcttggtct gtgggttgcc gcacaggttg
    42181 ccggttccac caacatcagc aacattaccg acaccgttta tcactggccg ctgagccaaa
    42241 gcatcccgct ggtgctggcg ctctgcgcct gtgcgttcgc cacttttatc gaaatgggca
    42301 aactgccgtt cgacctggcg gaagccgagc aggagttgca ggaaggtccg ctctccgaat
    42361 acagcggtag cggctttggc gtcatgaaat ggggtatcag cctgaaacag ctggtggtgt
    42421 tgcagatgtt cgtcggggtg tttattccgt ggggacaaat ggaaaccttc accgtcggtg
    42481 gactgctgct ggcgctggtg attgccatcg tcaaactggt ggtcggcgtc ctggttatcg
    42541 cgctgttcga aaacagcatg gcccgtctgc gtcttgatat tactccgcgc attacctggg
    42601 ctgggtttgg ctttgcattt ttagcgttcg tctccttgct ggcggcgtga ttaaagagag
    42661 tttgagcatg tctgaagaaa aattaggtca acattatctc gccgcgctga atgaggcatt
    42721 tccaggcgtc gtgctggacc acgcctggca gaccaaagat cagctgactg tcaccgtaaa
    42781 ggtgaactat ctgccggaag tggtggagtt tctctactac aagcagggtg gctggctgtc
    42841 ggtgctgttt agtaacgacg aacgcaaact gaatggtcat tacgccgttt attacgtgct
    42901 gtcgatggag aagggcacca agtgttgggt aacggttcgc gtcgaagttg acgccaacaa
    42961 accggagtat ccgtccgtga cgccgcgcgt tccggcggcg gtgtggggcg agcgcgaagt
    43021 gcgcgatatg tacggtttga ttccggttgg tctgccggat gaacgtcgtc tggtgctgcc
    43081 ggatgactgg ccggatgaac tttatccgct gcgtaaagac agcatggatt atcgtcagcg
    43141 tccggcaccg actaccgatg ctgaaaccta cgagttcatc aacgaactgg gcgacaagaa
    43201 aaacaacgtc gtgccgattg gtccgctgca cgtcacttct gacgaaccgg gtcacttccg
    43261 tctgttcgtc gatggcgaaa acattatcga cgccgactac cgtctgttct acgtccatcg
    43321 cggtatggaa aaactggcgg aaacccgcat gggttataac gaagtgacct tcctctctga
    43381 ccgtgtgtgc gggatctgcg gctttgccca cagcaccgcc tacaccacgt cggtggaaaa
    43441 cgcgatgggt attcaggtgc cagaacgcgc gcagatgatc cgcgccattc tgctggaggt
    43501 ggaacgcctg cactcgcatc tgctcaacct cggcctcgcc tgtcacttta ccggcttcga
    43561 ctccggcttt atgcagttct tccgcgtgcg tgaaacctcc atgaaaatgg cagagatcct
    43621 taccggtgcg cgtaaaacct acggcctgaa cctgatcggc gggattcgtc gcgatctgct
    43681 gaaagatggc atgatccaga cccgccagct ggcgcaacaa atgcgtcgtg aagtgcagga
    43741 gctggtggat gtgctgctga gtacgccgaa catggaacaa cgcaccgtcg gcattggtcg
    43801 tctggacccg gaaatcgccc gcgatttcag taacgttggc ccgatggttc gcgccagcgg
    43861 tctcgcccgc gatactcgcg ccgatcaccc gtttgtcggt tatggcctgc tgccaatgga
    43921 agtccacagc gagcagggct gcgacgttat ttcccgtctg aaagtgcgaa tcaacgaggt
    43981 ctataccgcg ctgaatatga tcgactacgg tctggataac ctgccgggcg gtccgctgat
    44041 ggtggaaggc tttacctaca ttccgcaccg tttcgcgctg ggctttgccg aagcgccgcg
    44101 cggcgacgat atccactgga gcatgaccgg cgacaaccag aagctgtacc gctggcgctg
    44161 ccgtgccgcg acctacgcga actggccgac cctgcgctac atgctgcgtg gcaataccgt
    44221 ttccgatgcg ccgctgatta tcggtagcct ggacccttgc tactcctgta ccgaccgcat
    44281 gaccgtagtc gatgtgcgta agaagaagag caaagtggtg ccgtacaaag aactcgagcg
    44341 ttacagcatt gagcgtaaaa actcgccgct gaaataagga atcgccatgt ttacctttat
    44401 caaaaaagtc atcaaaaccg gcacggcgac ctcgtcttat ccgctggagc cgattgcggt
    44461 tgataaaaac ttccgtggta agccagagca gaacccgcag cagtgcatcg gctgcgcggc
    44521 ttgcgtcaat gcctgcccgt caaacgcctt aacggttgaa actgacctcg ccacaggaga
    44581 gctggcgtgg gagtttaatc tcgggcgctg catcttctgt ggacgctgcg aagaagtttg
    44641 cccgacggcg gcgatcaaac tgtcgcaaga gtacgaactg gcggtgtgga agaaagaaga
    44701 cttcctgcaa cagtcccgct tcgcgctgtg caactgccgc gtctgcaatc gtcctttcgc
    44761 cgtacagaaa gagatcgact acgccattgc gctgcttaag cacaatggtg acagccgcgc
    44821 ggaaaaccac cgcgaaagct ttgagacttg cccggaatgt aagcgccaga aatgcctggt
    44881 gccgtccgac cgtattgaac tgactcgcca tatgaaagag gccatctgat gagcaattta
    44941 ttaggccccc gtgacgccaa cggcattccg gtcccgatga cggtggatga atccatcgcc
    45001 agcatgaagg cgtcgttact gaaaaaaatc aaacgttctg cctatgttta ccgcgtggac
    45061 tgcggcggct gcaatggctg cgaaatcgaa attttcgcca cgctttctcc gctgttcgat
    45121 gcagaacgct tcggcattaa agtcgttcct tcaccgcgtc atgcggatat tttactgttt
    45181 accggcgcgg tcacccgtgc aatgcgatcc cctgcgctgc gtgcgtggca gtccgcgccg
    45241 gacccgaaaa tctgtatctc atacggtgcc tgcggtaaca gcggcgggat cttccacgat
    45301 ctctactgcg tgtggggcgg tacggataaa attgtccctg tggatgttta tatccctggc
    45361 tgcccgccaa cgcctgccgc cacgctgtac ggctttgcaa tggcgctcgg cctgctggag
    45421 cagaaaattc acgcccgtgg gccgggtgaa ctggatgaac aaccggcgga gatcctgcat
    45481 ggcgatatgg tgcagccgct gcgcgtgaaa gtggatcgcg aagcacgtcg cttggcgggt
    45541 tatcgttacg gtcgtcagat tgccgatgat taccttacac agttagggca gggcgaagaa
    45601 caggttgcac gctggctgga agcggaaaac gatccgcgtc tgaacgagat tgtcagccat
    45661 ctgaatcatg ttgttgaaga ggcgcgtatc cgatgagtga aaaggtggtg ttcagtcaac
    45721 tgagccgtaa atttattgat gagaacgatg ccacgcccgc cgaggcgcag caggtggtct
    45781 attacagcct ggcgattggt caccaccttg gggttatcga ttgcctggaa gcggcgctca
    45841 cctgcccatg ggatgaatat ctggcatgga ttgccactct ggaggcgggc agcgaagccc
    45901 gccacaaaat ggaaggcgta ccgaaatatg gcgagatcgt catcgacatt aaccatgtgc
    45961 cgatgctggc caacgcattc gataaagccc gggcagcgca aacttcgcag cagcaggaat
    46021 ggagtacaat gctgttaagt atgctgcatg atattcatca ggaaaacgcc atctatttga
    46081 tggtgaggag actgcgtgac tgacgtttta ctctgtgttg gcaatagcat gatgggcgat
    46141 gatggcgctg gcccgctgct ggcggaaaag tgcgccgccg cgccgaaagg taactgggtg
    46201 gtgattgacg gcggtagtgc accggaaaac gacatcgtcg ccatccgtga actgcgcccg
    46261 acacgactgc tgattgtcga cgccacggat atggggctaa accccggcga aatccgcatc
    46321 atcgacccgg atgatatcgc cgagatgttt atgatgacta cccataacat gccgttgaat
    46381 taccttatcg accagttaaa agaagacatt ggtgaagtga ttttcctcgg cattcagccg
    46441 gatatcgtcg gcttttacta cccgatgacc cagccgatta aagatgcggt agaaaccgtt
    46501 tatcaacgac tggaaggctg ggaaggaaat ggtggatttg cgcagttagc ggtggaagaa
    46561 gaataattct ccatcaggga attcagggaa ttgaacatca gaacagggat gtgcgctttt
    46621 ttattgagct tagtgctgcc tgcacaggcc accagcttta ccgaatatct gccaatgagt
    46681 gacagcgaat acgcgcaaaa gcgggcgctg aaacctctcc tgaccatgcc ttatgatgcc
    46741 gagcaaacct ggcattttag gaaagtgggc gttgctggcg tgacgcttga gaagatgcca
    46801 aatgaagaag attactggca attaaccgct aaagaccgcg ccggtaaatc ctggactgtg
    46861 cctgtgggta tgctggcaaa catagcgggc aaagggcaac tctatcgcgc cgatcttgat
    46921 cgtaacggca ttcaggatct ggttatctgg ttatctggtt accaagtacc ggtaatggtc
    46981 tggctcctta tgcacattta attcttatga cgttcacgcg cgaaggccgt ccctgtgtct
    47041 ttgaacccag gggactctac accgcaagca aaactggcgt agatgatttg ctggatttgc
    47101 aaggcaatgg tcacacgcaa ttgctggata tgcagtttaa ttctggttac tggataacca
    47161 gtctgtatca ggtaaaagac gccaaatggc agcgtgttca cggctggttt gacaagttaa
    47221 gctatcccgc gctcacgcgt tttacttata cccccaaccg taaactcgtc ctcaaaccca
    47281 tcgccgggcg cgacccgcaa acggaagatc tggcgcagac gcagcgttgc ttgataaagg
    47341 gggatgtgct tgaggggtga accagaactg actttttgca gagtggctat gaggattttg
    47401 ggggtaatta tatcgcgcac aagtgtttac atattgtgaa cttggatttg atgagctata
    47461 gtttccgaac acataaggat agaactcgca gatgtggctg tttacttact ataaggaagt
    47521 taaaacttaa aaatgcacat catttctaaa gcgccatttg aagagtctgc gcgtaaatat
    47581 cctaacgacg ctctggctct tcaggcattg tatcgggtta ttaaggaaac ggatttttct
    47641 acgccagaag agatgcgaac ggcgtttccc aacctcgaca attttaaata tcgcaacaaa
    47701 tggtacgttc tggatgtagg tgggaacaac ctaagggtga ttgcctacat aaactttgtt
    47761 aataagcggt tctttgtgaa gcacataact aatcacgccg agtatgacaa actgacacgt
    47821 tactacagag agaacaaaga atgactgcta atgctgcacg cgctgttaaa gcaacaagag
    47881 aactggtcaa tgcagtcccc tttctgggag gtagtgactc agaagatgat tatcgtgaag
    47941 cactggaact ggtcgaatat ttgattgaag aagatgacac caatccactt atcgactttc
    48001 tggcgagccg cattgcagaa tatgaaaaca acaaccaaga gtttgcagag ttcgacaaag
    48061 ccgttgcggc aatgcctgtt ggcgtggcgt tacttcgcac gctgattgat caacataatc
    48121 tgacctatgc ggatttgaag aacgaaatcg gctctaaatc cctggtcagt cagattttgt
    48181 ctggacaacg atccctgacg atatcccaca ttaaggcact ttcagcccga tttggcgtta
    48241 agccggaatg gtttctttga tttttgacta atgccaaatg ctaaattgcc cgatgcgctg
    48301 cgcttatcgg gcctacatgt cgcacgaact atgtaggccg gataaggcgt tcacgccgca
    48361 tccggcactg ttacttactc taaatcttcc ccattactgg caatcacttt tttataccac
    48421 cagaacgatt tcttacgtgt gcgcgtcagc gtgccgttgc ctgcgtcgtc acggtctacg
    48481 tagacaaaac cgtagcgttt gctcatttca cccgtagagg cggaaactaa atcaatacag
    48541 ccccatgtgg tgtagcccat cagcggaatg ccatctgcaa tcgcttcgcc cattgcgcgg
    48601 atatgttcgc gtaaatagct gatgcgatag tcatcgttaa tctcgccatt ggcagcaagt
    48661 tcatcttttg cgccgaggcc gttttccacc agaaacagcg gcttctgata acgatcgtac
    48721 atcatattca tggtgatacg cagaccgagt ggatcaattc cccagcccca gtcgctcact
    48781 tgcagatacg ggttacgcag cgatttcacc acgttcgccg cactgctgtt gttggcgttc
    48841 atctccgccg aggcgcagcg cgaggcgtaa tagctgaaag agacaaaatc gacggtgttt
    48901 ttcaggatct catcatcgcc cagcgcttta tctatggtta cccctttttc gcgaaatacg
    48961 cgggcagagt aagccggata cgcgccgcgc gcctgcacat cgataaaaaa caggttttcc
    49021 cgatctttct ccagcgccgc ccagacatct tccggtttgc agctgtaagg gtagaagtta
    49081 ccgcccgcca gcatacagcc cacctgattt tgcgggttaa cctcatgggc gattttggtg
    49141 gctagcgcac tggcaaccag ctggtgatgc gcggcctgat atttcacctg atcctgattt
    49201 tcaccttctt caaacaccag acccgcgccg gagaacgggc tatgcaacat aatgttgatt
    49261 tcattgaagg ttagccagta tttcaccaga ccatcaaatg cttcaaagca ggttctggcg
    49321 tagcggctga aaaactccac cagcttgcgg ttacgccagg agccatattc ggtgaccaga
    49381 tgcatcggca catcgaagtg gcacaacgtg accagcggtt cgataccgta ctttttacac
    49441 tcttcaaaga caggacgata aaaagcaatg ccctgctgat tgggcgtgat ttcatcgccc
    49501 tgcggaaaga gacggctcca ggcaattgag gtacggaaaa ccttgaatcc catctctgcc
    49561 atcagggcga tatcttcttt ataacgatga taaaaatccg tcgcctcatg gctgggataa
    49621 aactcgtcat cgcgcaactg aaaacgtttt tccagcccca gtttcaccgc cattcgatgc
    49681 tcgccgtgtg ggatcatatc gacggtggtc agcccttttc cgccttcacg gaacgcacct
    49741 tcagactggt tggcggcaag cgcgccgccc cataaaaaac cttctggaaa tactgacatt
    49801 ttcatcctca attaagactt acttctttgg cgacggtcgg ttgtacgctc tgatgctttc
    49861 gcgcctgggc agccgcttcc tcaacaggaa tatcctcaaa gccgagcaac agtgtgagga
    49921 taaacgacag catcaccgcc agcgccatga ccgcgaacac ccagacgatg ctcattggat
    49981 tcgccggatc gaagaattgc acgctggtaa atagccccg
//
LOCUS       DADLSA010000011        49492 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.011, whole genome shotgun sequence.
ACCESSION   DADLSA010000011 DADLSA010000000
VERSION     DADLSA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.011"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(129..608)
                     /gene="folK"
                     /locus_tag="JN691_000686"
     CDS             complement(129..608)
                     /gene="folK"
                     /locus_tag="JN691_000686"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAY7242202.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="JN691_000687"
     CDS             complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="JN691_000687"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAY7242203.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLRMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="JN691_000688"
     CDS             complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="JN691_000688"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706092.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAY7242204.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILLSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3025..3480)
                     /locus_tag="JN691_000689"
     CDS             complement(3025..3480)
                     /locus_tag="JN691_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAY7242205.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="JN691_000690"
     CDS             complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="JN691_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAY7242206.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="JN691_000691"
     CDS             complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="JN691_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAY7242207.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4981..7410
                     /gene="hrpB"
                     /locus_tag="JN691_000692"
     CDS             4981..7410
                     /gene="hrpB"
                     /locus_tag="JN691_000692"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAY7242208.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQSLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAGILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7606..10140
                     /gene="mrcB"
                     /locus_tag="JN691_000693"
     CDS             7606..10140
                     /gene="mrcB"
                     /locus_tag="JN691_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAY7242209.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10360..12603
                     /gene="fhuA"
                     /locus_tag="JN691_000694"
     CDS             10360..12603
                     /gene="fhuA"
                     /locus_tag="JN691_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAY7242210.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPVPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKNGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12654..13451
                     /gene="fhuC"
                     /locus_tag="JN691_000695"
     CDS             12654..13451
                     /gene="fhuC"
                     /locus_tag="JN691_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAY7242211.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13451..14341
                     /gene="fhuD"
                     /locus_tag="JN691_000696"
     CDS             13451..14341
                     /gene="fhuD"
                     /locus_tag="JN691_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAY7242212.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14338..16320
                     /gene="fhuB"
                     /locus_tag="JN691_000697"
     CDS             14338..16320
                     /gene="fhuB"
                     /locus_tag="JN691_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAY7242213.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16355..17635)
                     /gene="hemL"
                     /locus_tag="JN691_000698"
     CDS             complement(16355..17635)
                     /gene="hemL"
                     /locus_tag="JN691_000698"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAY7242214.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEESGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17860..19281
                     /gene="clcA"
                     /locus_tag="JN691_000699"
     CDS             17860..19281
                     /gene="clcA"
                     /locus_tag="JN691_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAY7242215.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19305..19370
                     /locus_tag="JN691_000700"
     CDS             19305..19370
                     /locus_tag="JN691_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242216.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19363..19707
                     /gene="erpA"
                     /locus_tag="JN691_000701"
     CDS             19363..19707
                     /gene="erpA"
                     /locus_tag="JN691_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAY7242217.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19754..20377)
                     /locus_tag="JN691_000702"
     CDS             complement(19754..20377)
                     /locus_tag="JN691_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HAY7242218.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20415..21215)
                     /gene="btuF"
                     /locus_tag="JN691_000703"
     CDS             complement(20415..21215)
                     /gene="btuF"
                     /locus_tag="JN691_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAY7242219.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21208..21906)
                     /gene="mtnN"
                     /locus_tag="JN691_000704"
     CDS             complement(21208..21906)
                     /gene="mtnN"
                     /locus_tag="JN691_000704"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAY7242220.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGCLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNIPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21990..23507
                     /gene="dgt"
                     /locus_tag="JN691_000705"
     CDS             21990..23507
                     /gene="dgt"
                     /locus_tag="JN691_000705"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY7242221.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRINLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23637..25061
                     /gene="degP"
                     /locus_tag="JN691_000706"
     CDS             23637..25061
                     /gene="degP"
                     /locus_tag="JN691_000706"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAY7242222.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25216..26372
                     /locus_tag="JN691_000707"
                     /pseudo
     CDS             25216..26372
                     /locus_tag="JN691_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
     gene            complement(26461..26847)
                     /locus_tag="JN691_000708"
     CDS             complement(26461..26847)
                     /locus_tag="JN691_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAY7242223.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27161..27985)
                     /gene="dapD"
                     /locus_tag="JN691_000709"
     CDS             complement(27161..27985)
                     /gene="dapD"
                     /locus_tag="JN691_000709"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAY7242224.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28016..30688)
                     /gene="glnD"
                     /locus_tag="JN691_000710"
     CDS             complement(28016..30688)
                     /gene="glnD"
                     /locus_tag="JN691_000710"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAY7242225.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30750..31544)
                     /gene="map"
                     /locus_tag="JN691_000711"
     CDS             complement(30750..31544)
                     /gene="map"
                     /locus_tag="JN691_000711"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAY7242226.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31912..32637
                     /gene="rpsB"
                     /locus_tag="JN691_000712"
     CDS             31912..32637
                     /gene="rpsB"
                     /locus_tag="JN691_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAY7242227.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32895..33746
                     /gene="tsf"
                     /locus_tag="JN691_000713"
     CDS             32895..33746
                     /gene="tsf"
                     /locus_tag="JN691_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAY7242228.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33893..34618
                     /gene="pyrH"
                     /locus_tag="JN691_000714"
     CDS             33893..34618
                     /gene="pyrH"
                     /locus_tag="JN691_000714"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAY7242229.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34910..35467
                     /gene="frr"
                     /locus_tag="JN691_000715"
     CDS             34910..35467
                     /gene="frr"
                     /locus_tag="JN691_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAY7242230.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35559..36755
                     /gene="ispC"
                     /locus_tag="JN691_000716"
     CDS             35559..36755
                     /gene="ispC"
                     /locus_tag="JN691_000716"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAY7242231.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36944..37702
                     /gene="ispU"
                     /locus_tag="JN691_000717"
                     /gene_synonym="uppS"
     CDS             36944..37702
                     /gene="ispU"
                     /locus_tag="JN691_000717"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAY7242232.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37715..38572
                     /gene="cdsA"
                     /locus_tag="JN691_000718"
     CDS             37715..38572
                     /gene="cdsA"
                     /locus_tag="JN691_000718"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY7242233.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38584..39936
                     /gene="rseP"
                     /locus_tag="JN691_000719"
     CDS             38584..39936
                     /gene="rseP"
                     /locus_tag="JN691_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAY7242234.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMFVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39966..42398
                     /gene="bamA"
                     /locus_tag="JN691_000720"
     CDS             39966..42398
                     /gene="bamA"
                     /locus_tag="JN691_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAY7242235.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42520..43005
                     /gene="skp"
                     /locus_tag="JN691_000721"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42520..43005
                     /gene="skp"
                     /locus_tag="JN691_000721"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAY7242236.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43009..44034
                     /gene="lpxD"
                     /locus_tag="JN691_000722"
     CDS             43009..44034
                     /gene="lpxD"
                     /locus_tag="JN691_000722"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAY7242237.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44139..44594
                     /gene="fabZ"
                     /locus_tag="JN691_000723"
     CDS             44139..44594
                     /gene="fabZ"
                     /locus_tag="JN691_000723"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAY7242238.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44598..45386
                     /gene="lpxA"
                     /locus_tag="JN691_000724"
     CDS             44598..45386
                     /gene="lpxA"
                     /locus_tag="JN691_000724"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAY7242239.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45386..46534
                     /gene="lpxB"
                     /locus_tag="JN691_000725"
     CDS             45386..46534
                     /gene="lpxB"
                     /locus_tag="JN691_000725"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAY7242240.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46531..47127
                     /gene="rnhB"
                     /locus_tag="JN691_000726"
     CDS             46531..47127
                     /gene="rnhB"
                     /locus_tag="JN691_000726"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAY7242241.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47164..>49492
                     /gene="dnaE"
                     /locus_tag="JN691_000727"
     CDS             47164..>49492
                     /gene="dnaE"
                     /locus_tag="JN691_000727"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAY7242242.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLTFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKA"
BASE COUNT        11585 a        12215 c        13762 g        11930 t
ORIGIN      
        1 atgggattga tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta
       61 aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata
      121 taaataaatt accatttgct taatttgtca aatgctcttg tatgtaagac ttcacgcaac
      181 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
      241 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      301 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      361 cgaccctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgggcgaa
      421 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg
      481 gtgcggtaaa acgaagaaac ggcaagaatg cggctttcag ggatatcgcc taatgctttc
      541 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      601 actgtcatgt ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacgacgac
      661 gcggtgacgg ttcttcatcc agctcgttaa gcatcccttt ttgatctggt ggtgcggaaa
      721 cctggaactc accccaccat ttcaccagac gctgcagttc agcattacgt tcaacttcag
      781 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      841 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      901 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      961 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
     1021 ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt
     1081 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1141 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1201 cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1261 caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg
     1321 cggtttccgg gctgatgcgc atacgcaatt tggcggcaaa acgtaccgcg cgcagcatac
     1381 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1441 tcagatcctt cataccgcca acgtaatcac ggacggtaaa atccgctacg ctgtaataca
     1501 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1561 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1621 cgtggtgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca
     1681 gacggaaacg gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1741 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1801 cgccgccaac cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg
     1861 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1921 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1981 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     2041 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2101 cgactcgggc acggcggtca gcatccagtt tttgacggct gacagaagga tttgctcgac
     2161 gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag
     2221 taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2281 gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggaactttcc agccaaaaag
     2341 ctggtacagc gagatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2401 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2461 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgcctcg
     2521 cagcaggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc
     2581 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg
     2641 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2701 acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2761 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2821 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga gcctgcaaat agctgccgag
     2881 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2941 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     3001 gtaaacgtga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     3061 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3121 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3181 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3241 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3301 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3361 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3421 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3481 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3541 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3601 aggaaaagcg tatttgcgct cgcgaccaga atgtaaatta accagttact tactttacta
     3661 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta
     3721 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3781 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac
     3841 cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc
     3901 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3961 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcgga
     4021 gccgtatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     4081 aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc
     4141 gctctggctt tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4201 cgaataccag acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca
     4261 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4321 ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat
     4381 tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4441 gcgtaaaggg tgaactctgt caccgcataa gaccagttaa aacccggcgg cgggaatgtc
     4501 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4561 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4621 ggaggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca
     4681 agcgtgagtg tgaaaccagg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4741 ttctctgcgc tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc
     4801 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg ttcgcggatt
     4861 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4921 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4981 gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg
     5041 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5101 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     5161 gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt
     5221 ggctaccgga tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtggttacc
     5281 gaaggcgtgc tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg
     5341 atccttgatg agtttcatga gcgcagtttg caggcggatt tggcgttggc gctgttactc
     5401 gatgtgcaac aaagtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac
     5461 aatgaccgct tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga agggcgctcg
     5521 tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgaagccgtt
     5581 gcggtagcca ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt atttttacct
     5641 ggcgtcggag aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg cagtgatgtg
     5701 gtgctctgcc cactgtatgg cgcgttgtca ctgaacgatc agcgaaaagc gatcctcccg
     5761 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5821 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ctttgatccg
     5881 cgcacggggc ttacgcggtt gattactcaa cgcgttagtc aggcatccat gacgcagcgt
     5941 gccgggcgcg ccgggcgtct ggagccgggt atcagcctgc atttaatcgc caaagaacaa
     6001 gcagaacgcg ccgcggcgca aagtgagccg gagatcttac aaagcgatct ttccggtttg
     6061 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     6121 ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag
     6181 ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6241 gcggcaatgc tggttaacgc aaagagcgat gacgaagctg ctaccgcggc aaaaattgcc
     6301 ggcattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc
     6361 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc
     6421 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6481 gctcatcgcc gtgggcaaga tggacgctat caactggcaa acggcatggg agcgatgctc
     6541 gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc
     6601 agcgcctcgc cggatgcgcg gattttactg gcgctaccgg tcgatattga tgaattagta
     6661 caacgctgtc cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt
     6721 acgctgaaag cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt gcagccgctg
     6781 gcgaaaccgt cagaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt
     6841 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6901 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt gttggcgacg
     6961 ctggaaacgt ggctgctgcc acatatgacc ggcgtacatt cactacgcgg cctgaaatca
     7021 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     7081 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7141 gaagataacc cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat
     7201 ccgacgatcg cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc tgcccaaagg
     7261 ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa
     7321 aaggagatga aagggcgtta tcccaaacac gtctggccgg acgacccggc aaacacggca
     7381 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7441 gggccggggt gagggcatca gcgcgcacgt tcaccctcac cctaaccctc tccctcaagg
     7501 gagaggggac cgatcgagca caaattttga gagatatctt cttctgtctt gtaacagaag
     7561 aacagaaaat cgggcttttg cgcctgaata ttgcggagaa aaagcatggc cgggaatgac
     7621 cgcgagccaa ttggacgcaa agggaaaccg acgcgtccgg tcaaacaaaa ggtaagccgt
     7681 cgtcgttacg aagatgacga tgattacgac gattatgatg actatgagga tgaagaaccg
     7741 atgccgcgca aaggtaaggg caaaggcaaa gggcgtaagc ctcgtggcaa acgcggctgg
     7801 ctatggctac tgctaaaact ggctatcgtt tttgccgtgc tgatcgccat ttacggcgtt
     7861 tatctcgatc aaaaaattcg tagccgtatt gatggcaagg tctggcaact gcccgcggca
     7921 gtttatggcc gaatggtcaa ccttgaacca gatatgacca tcagcaagaa cgaaatggtg
     7981 aagctgctgg aggcaacaca gtatcgtcag gtgtcgaaaa tgacccgtcc tggcgaattt
     8041 accgtgcagg ccaacagcat tgagatgatc cgccgtccgt ttgatttccc ggacagtaaa
     8101 gaagggcagg tgcgtgcgcg tctgaccttt gatggtgatc atctggcgac catcgtcaat
     8161 atggagaaca accgtcagtt cggtttcttc cgtcttgatc cgcgtctgat caccatgatc
     8221 tcctcgccaa acggtgagca gcgtttgttt gtgccgcgca gtggtttccc tgatttgctg
     8281 gtggatactt tgctggcgac agaagaccgt catttttacg agcatgatgg aatcagtctc
     8341 tactccattg ggcgtgcggt gctggcaaac ctgaccgcag gtcgcacggt gcagggtgcg
     8401 agtacgctga cgcaacagct ggtgaaaaac ctgttcctct ccagcgagcg ttcttactgg
     8461 cgtaaagcga acgaagctta catggcgctg atcatggacg cgcgttacag caaagaccgt
     8521 attcttgagc tgtatatgaa cgaggtgtat ctcggtcaga gcggcgacaa cgaaatccgc
     8581 ggcttcccgc tggcaagctt gtattacttt ggccgcccgg tagaagagct aagcctcgac
     8641 cagcaggcgc tgttagtcgg tatggtgaaa ggggcgtcca tctacaaccc gtggcgtaac
     8701 ccgaaactgg cgctggagcg acgtaatctg gtgctgcgtc tgctgcaaca gcaacagatt
     8761 attgatcaag aactctatga catgttgagt gcccgtccgc tgggggttca gccgcgtggt
     8821 ggggtgatct ctcctcagcc agcctttatg cagctggtgc gtcaggagtt gcaggcaaaa
     8881 ctgggcgata aggtaaaaga tctctccggc gtgaagatct tcactacctt tgactcggtg
     8941 gcccaggacg cggcagaaaa agccgccgtg gaaggcattc cggcactgaa gaaacagcgt
     9001 aagttgagcg atcttgaaac tgcgattgtg gtcgtcgacc gctttagtgg tgaagttcgt
     9061 gcgatggtcg gaggttctga gccgcagttt gcgggctaca accgtgcgat gcaggcgcgt
     9121 cgttcgattg gttcccttgc aaaaccagcg acttatctga cggctttaag ccagccgaaa
     9181 atctatcgcc tgaacacgtg gattgccgat gcgccaatcg cgctgcgtca gccgaatggc
     9241 caggtctggt caccgcagaa cgatgaccgt cgttatagcg aaagcggcag agtgatgctg
     9301 gtggatgcgt tgacacgttc gatgaacgtg ccgacggtaa atctggggat ggcgctgggg
     9361 ctgcctgcgg ttacggagac ctggatcaaa ctgggcgtac cgaaagatca gttgcatccg
     9421 gttccggcaa tgctgctggg ggcgttgaac ttaacgccaa tcgaagtggc gcaggcattc
     9481 cagaccatcg ccagcggtgg taaccgtgca ccgctttctg cactgcgttc ggtaatcgcg
     9541 gaagatggca aggtgctgta tcagagcttc ccgcaggcag aacgcgctgt tccggcgcag
     9601 gcggcgtatc tgacactatg gaccatgcag caggtggtac aacgtggtac gggtcgtcag
     9661 cttggggcga aatacccgaa cctgcatctg gcagggaaaa cagggactac caacaataac
     9721 gtagatacct ggtttgcagg cattgacggc agcacggtga ccatcacctg ggtaggccgt
     9781 gataacaacc agccgaccaa actgtatgga gccagcgggg caatgtcgat ttatcagcgt
     9841 tatctggcta accagacgcc aacgccgctg aatcttgttc cgccagaaga tattgcagat
     9901 atgggcgtgg actacgacgg caactttgtt tgcagcggtg gcatgcgtgt cttgccggtc
     9961 tggaccagcg atccgcaatc gctgtgccag cagagcgaga tgcagcagca gccgtcaggc
    10021 aatccgtttg atcagtcttc tcagccgcag caacagccgc aacagcaacc tgctcagcaa
    10081 gagcagaaag acagcgacgg tgtagccggt tggatcaagg atatgtttgg tagtaattaa
    10141 catctaagcg tgaaataccg gatggcgagt tgccatccgg taaaataaca tcccatctaa
    10201 gatattaacc ctttcttttc atctggttgt ttattaaccc ttcaggaacg ctcagattgc
    10261 gtaccgcttg cgaacccgcc agcgtttcga atattatctt atctttataa taatcattct
    10321 cgtttacgtt atcattcact ttacatcaga gatataccaa tggcgcgttc caaaactgct
    10381 cagccaaaac actcactgcg taaaatcgca gttgtagtag ccacagcggt tagcggcatg
    10441 tctgtttatg cacaggcagc ggttgaaccg aaagaagaca ctatcaccgt taccgctgca
    10501 cctgtgccgc aagaaagcgc atgggggcct gctgcaacta ttgcggcgcg acagtcagct
    10561 accggcacta aaaccgatac gccgattcaa aaagtgccac agtctatttc tgttgtgacc
    10621 gccgaagaga tggcgctgca tcagccgaag tcggtaaaag aagcgcttag ctacacgccg
    10681 ggtgtctctg ttggtacgcg tggcgcatct aacacctatg accacctgat cattcgcggt
    10741 tttgcggcag aaggccaaag ccaaaataac tatctgaatg gcctgaagtt gcagggcaac
    10801 ttctataacg atgcggtcat tgacccgtat atgctggaac gcgctgaaat tatgcgtggc
    10861 ccggtttccg tgctttacgg taaaagcagt cctggcggtc tgttgaatat ggtcagcaag
    10921 cgtccgacca ccgaaccgct gaaagaagtt cagtttaaag ccggtactga cagcctgttc
    10981 cagactggtt ttgactttag cgatgcgttg gatgatgacg gtgtttactc ttatcgcctg
    11041 accggtcttg cgcgttctgc caatgcccag cagaaagggt cagaagagca gcgttatgct
    11101 attgcaccgg cgttcacctg gcgtccggat gataaaacca atttcacctt cctttcttac
    11161 ttccagaacg agccggaaac cggttattac ggctggttgc cgaaagaggg aaccgttgag
    11221 ccgctgccga acggtaagcg tctgccgaca gactttaatg aaggggcgaa gaacaacacc
    11281 tattctcgta atgagaagat ggtcggctac agcttcgatc acgaatttaa cgacaccttt
    11341 actgtgcgtc agaacctgcg ctttgctgaa aacaaaacct cgcaaaacag cgtttatggt
    11401 tacggcgtct gctccgatcc ggcgaatgct tacagcaaac agtgtgcggc attagcgcca
    11461 gcggataaag gccattatct ggcacgtaaa tacgtcgttg atgatgagaa gctgcaaaac
    11521 ttctccgttg atacccagtt gcagagcaag tttgccactg gcgatatcga ccacaccctg
    11581 ctgaccggtg tcgactttat gcgtatgcgt aatgacatca acgcctggtt tggttacgac
    11641 gactctgtgc cactgctcaa tctgtacaat ccggtgaata ccgatttcga cttcaatgcc
    11701 aaagatccgg caaactccgg cccttaccgc attctgaata aacagaaaca aacgggcgtt
    11761 tatgttcagg atcaggcgca gtgggataaa gtgctggtca ccctaggcgg tcgttatgac
    11821 tgggcagatc aagaatctct taaccgcgtt gctgggacga ccgataaacg tgatgacaaa
    11881 cagtttacct ggcgtggtgg tgttaactac ctgtttgata atggtgtaac accttacttc
    11941 agctatagcg aatcgtttga accttcttcg caagttggga agaatggtaa tattttcgca
    12001 ccgtctaaag gtaagcagta tgaagtcggc gtgaaatatg taccggaaga tcgtccgatt
    12061 gtagttactg gtgccgtgta taatctcact aaaaccaaca acctgatggc ggaccctgag
    12121 ggttccttct tctcggttga aggtggcgag atccgcgcac gtggcgtaga aatcgaagcg
    12181 aaagcggcgc tgtcggcgag tgttaacgta gtcggttctt atacttacac cgatgcggaa
    12241 tacaccaccg atactaccta taaaggcaat acgcctgcac aggtgccaaa acacatggct
    12301 tcgctgtggg ctgactatac cttctttgac ggtccgcttt caggtctgac gctgggcacc
    12361 ggtggtcgtt atactggctc cagctatggt gatccggcta actcctttaa agtgggaagt
    12421 tatacggtcg tggatgcgtt agtgcgttat gatctggcgc gagtcggcat ggcgggctcc
    12481 aacgtggcgc tgcatgttaa caacctgttc gatcgtgaat acgtcgccag ctgctttaac
    12541 acttatggct gcttctgggg cgcagaacgt caggtcgttg caaccgcaac ctttcgtttc
    12601 taatttctct tttggggcac ggatttccgt gcccatttca caagttggct gttatgcagg
    12661 aatacacgaa tcattccgat accacttttg cactgcgtaa tatctccttt cgtgtgcccg
    12721 ggcgcacgct tttgcatccg ctgtcgttaa cctttcctgc cgggaaagtg accggtctga
    12781 ttggtcacaa cggttctggt aaatccactc tgctcaaaat gcttggtcgt catcagcctc
    12841 cgtcagaagg ggagattctt cttgatgccc agccgctgga aagctggagc agcaaagcgt
    12901 ttgcccgcaa agtggcttat ttgccgcagc agcttcctcc ggcagaaggg atgaccgtgc
    12961 gtgaactggt ggcgattggt cgttacccgt ggcatggcgc gctggggcgc tttggtgcgg
    13021 cagatcgcga aaaggtcgag gaagctatct cgctggttgg cttaaaaccg ctggcgcatc
    13081 ggctggtcga tagtctctct ggcggcgaac gtcagcgggc gtggatcgcc atgctggtgg
    13141 cgcaggatag ccgttgtctg ttgctcgacg aaccgacttc ggcgctggat atcgcccacc
    13201 aggttgatgt gctggcgctg gtgcaccgtt taagtcagga acgtggcctg acggtcattg
    13261 ccgtgttgca cgatatcaat atggcggcac gctactgtga ttatctggtc gccctgcgca
    13321 gtggtgaaat gattgctcag ggaacgcctg cggaaattat gcgcggcgaa accctcgaaa
    13381 tgatttatgg catcccgatg ggtattttgc cgcatccggc gggtgctgca cctgtgagtt
    13441 ttgtttattg atgagcggct tacctcttat ttcgcgccgt cgactgttaa cggcgatggc
    13501 gctttctccg ttgttatggc agatgaatac cgcccacgcg gcggctattg accccaatcg
    13561 tattgtggcg ctggagtggt tgccggtgga attactgctg gcgctcggca tcgtgcctta
    13621 cggcgtggcg gataccatca actatcgcct gtgggtcagc gaaccaccat tgccggactc
    13681 agtgattgac gtcggtttgc gtacagaacc taaccttgaa ctgctgaccg aaatgaaacc
    13741 gtcgtttatg gtctggtcgg caggatatgg cccttcatca gaaatgctgg cacgtattgc
    13801 gccgggtcgc ggatttaact tcagtgacgg caaacatccg ttggcgatgg cgcgtaaatc
    13861 gctgacggaa atggcagatt tacttaacct gcaaagcgca gcggaaacgc atttagcgca
    13921 atacgaagac tttatccgca gcatgaaacc ccgctttgtg aagcgtggtg cacgcccgtt
    13981 actgctgacg acgcttatcg atccgcgcca tatgctggtc ttcggtccaa acagcttgtt
    14041 ccaggaaatt ctcgatgagt acggcatccc aaatgcctgg caaggggaaa ccaacttctg
    14101 gggcagtacc gccgtcagta tcgatcgtct ggcagcgtat aaagacgttg atgtgctctg
    14161 ttttgatcac gacaacagca aagacatgga tgcgctaatg gcaaccccgc tgtggcaggc
    14221 catgccgttt gtccgtacag ggcgctttca gcgcgtaccc gcagtctggt tttatggtgc
    14281 aacgctttcg gcaatgcact ttgtgcgtgt tctggataac gccatcggag gtaaagcgtg
    14341 agtaaacgaa ttgcgctttt cccggcgtta ttgctggcgc tgttagtgat tgtcgctacg
    14401 gcgctcacct ggatgaactt ctcacaggcg ctgccgcgca gtcagtgggc gcaggccgcc
    14461 tggtcgccgg atattgacgt catcgagcag atgatttttc actacagctt gttgccgcgt
    14521 ctggcgattt cgctgctggt gggcgcgggc ctggggctgg tgggcgtgct gtttcagcaa
    14581 gtactgcgta acccgctggc ggaaccgacg acgttgggcg ttgcaacagg cgcgcaactg
    14641 gggattaccg tcaccacgct ctgggcgatc cctagcgcga tggcgagcca gtttgctgcg
    14701 ctggcagggg cttgcgttgt tggcttaatt gtctttggcg tcgcgtgggg gaaacggctg
    14761 tcgccggtaa cgctgattct cgcggggttg gtagtgagcc tttattgcgg cgcaatcaat
    14821 cagttactgg ttatcttcca tcatgaccag ttgcaaagca tgtttctgtg gagcactgga
    14881 acgctgacgc aaaccgactg gggcggtgtt gagcgtttat ggccgcagtt gctgggcggt
    14941 gtgatgctga cgttgctgct acttcgtccg ttaaccctga tggggcttga tgatggcgtg
    15001 gcgcgcaatc tcgggctggc cttgtcgctt gcgcgtctgg cggcgctgtc gctggcgatt
    15061 gtcatcagtg cgctgctggt gaacgctgtg gggattatcg gctttatcgg gttgttcgcg
    15121 ccgctgctgg caaaaatgct gggggcgcgg cgtctgctgc cacgactgat gctggcgtcg
    15181 ttgattggtg cgctgatcct ctggctttcc gatcaaatca tcctctggct gactcgcgtg
    15241 tggatggaag tgtccaccgg ttcggtcact gcgttgatcg gtgcgccgct gctactgtgg
    15301 ctgctgccgc gtttacgcag cattagcgcg ccggatatga aggttaacga tcgtgtcgcg
    15361 actgaacgcc aacatgtgct ggcgtttgcc ctcgcgggcg gcgtgctgtt gttgatggct
    15421 gtggtggtgg cgctgtcgtt tggtcgtgat gcgcacggct ggacgtgggc gagcggggcg
    15481 ttgctcgagg atttaatgcc ctggcgctgg ccgcgaatta tggcggcgct gtttgcgggc
    15541 gtcatgctgg cggtagcggg ctgtattatt cagcgactga ccggcaaccc gatggcaagc
    15601 ccggaagtgc tggggattag ctccggcgcg gcgtttggtg tggtgttaat gctgtttctg
    15661 gtaccgggta atgcctttgg ctggctgtta cccgcaggca gtctcggggc ggcggtgacg
    15721 ctgttgatca ttatgatcgc cgccggacgc ggtggatttt ccccacaccg catgttactg
    15781 gcggggatgg cgttaagtac cgcgttcacc atgcttttga tgatgttgca ggcaagtggt
    15841 gacccgcgaa tggcgcaagt gctgacctgg atttccggtt ctacctacaa cgcgaccgat
    15901 gctcaggtct ggcgcaccgg aattgtgatg gtgattttgc tggcgattac tccgctgtgc
    15961 cgccgctggc tgaccatttt accgctgggt ggtgataccg cgcgggcggt agggatggcg
    16021 ctgacgccga cgcgaattgc gctgctgctg ttagcggctt gcctgacggc gaccgcgacg
    16081 atgactattg gaccgttgag ttttgttggt ttaatggcac cgcatattgc gcggatgatg
    16141 ggctttcgac ggacgatgcc acacatcgta atttcggcgc tggtgggtgg tttactgctg
    16201 gtgttcgctg actggtgtgg gcggatggtg ctgtttccat tccagatccc ggcggggctg
    16261 ctgtcgacct ttatcggtgc gccatatttt atctatttgc tgagaaagca gagccgttaa
    16321 tagaatgcct cgcccggtgg gcgaggcaaa attatcacaa cttcgcgaac acccgacgtg
    16381 cagcatcgat ggtgttattg atatcttcca tgctgtgcgc cacagacata aagcccgctt
    16441 caaacgctga cggtgccagg taaacacctt catccagcat catatggaag aaacgcttaa
    16501 aacgctcgac gtcacaggcc atcacatcct gatagcacgt cacggactcg gcgtcggtaa
    16561 agaagatacc gaacatacca ccgacgtggt taacgaccag cggaattccc gattcttctg
    16621 ccgcttccag cagaccttct gccagacgtg ttgtcagctc atccagcgtt tcgtgaatgc
    16681 ccggctgcgc gacttcattc agacaggcga aacccgctgc catcgcaatc gggttaccgg
    16741 aaagcgtacc cgcctgatag accggacctg tcggggccag cgcatccatt acatcacgac
    16801 gaccaccgaa tgcgcctacc ggcattccac cgccgatgat ttttccgagg caggttaaat
    16861 ctggcactac gccgaaataa tcctgtgcgc cagctagcgc tacgcggaaa ccggtcatca
    16921 cttcatcgat gatcagcaac gcgccaaatt cgtcgcacag cgcacgcaga cctggcagga
    16981 actctggcag cggcggaaca cagttcatat tgcctgccac cggctcgacg ataatacagg
    17041 caatctcttg cgggtattgc tcaaacgcgg cgcgtacaga ggccagatcg ttataagtac
    17101 aggttaaggt atgtttggcg aaatctgccg gaacgcccgg cgagtttggc tggcctaacg
    17161 tgagtgcgcc agaaccggct ttcaccagca ggcagtcagc gtgaccgtgg taacaacctt
    17221 caaatttaat gattttgtcg cgaccggtaa aaccacgggc caggcggatg gcgctcatcg
    17281 tcgcctcggt gccggagttc accatgcgca ccatatccat ggtcgggacc agttcggtca
    17341 ccagttgcgc cattttcact tccatttcgg tcggtgcacc aaagcttaaa ccacgctcgg
    17401 cggcttcaat cacggcattg cggatcgccg gatggttatg gcccagcacc atcggccccc
    17461 aggaaccgac ataatcgata taggctttgc catcaacatc gtacagataa gcgccgtccg
    17521 ctttttcgat aaacagtgga gtgccgccca cgccagtaaa ggcgcgaaca ggggagttca
    17581 caccgccagg gatcagctcg cgcgctgcgc tgtaaagatt ttcagactta ctcatggagg
    17641 gttcctgatt cgtagaaaaa gtgaatggct gctattctat gttattcata acaagttaaa
    17701 tactcgtcaa acatcaggct gcttgtactg gtgcaatcct gatttcttag agtataaaag
    17761 ttttgtgcat ttgaaacatt acgctttgca aaggattttc atggaacgtg cgagtaaaat
    17821 gccgtcatct tatttgtatg accaataagt gatcattgga tgaaaactga tactccctct
    17881 ttagaaacac cgcaggccgc gcgcctgcga cgcagacaac tgattcgcca acttcttgag
    17941 cgcgataaaa ccccgttagc cattttgttt atggcggcag tcgtcggcac gcttgtcggg
    18001 ctggctgcgg ttgcttttga caaaggtgtc gcctggttgc agaaccaacg tatgggggcg
    18061 ctggtacata ctgctgataa ttatccgcta ctgttaaccg tcgcttttct ctgttcggcg
    18121 gtgctggcga tgttcggcta ctttttggtg cgcaaatacg cgcccgaagc agggggatcg
    18181 gggatcccgg aaattgaagg ggcgctggaa gatcaacgtc ctgttcgctg gtggcgtgta
    18241 ttgccggtga aattctttgg cgggctgggg acactcggcg gaggcatggt gctagggcgc
    18301 gaaggaccaa ccgtgcagat cggcggcaat atcggtcgca tggtgcttga tattttccgc
    18361 ctgaaaggtg atgaagctcg ccatacgctg ctggcaaccg gtgctgctgc ggggctggct
    18421 gcggccttta acgcgccgct ggcgggtatt ttgtttatta tcgaagagat gcgtccgcag
    18481 tttcgctata cgttaatttc gattaaagcg gtatttattg gcgtcattat gtcgaccatt
    18541 atgtaccgga tttttaatca tgaagttgcg ttgattgacg tcggtaaact ttctgatgcg
    18601 ccgcttaata cgctgtggct ttatctgatc ctcggtatta tttttggcat tttcggccct
    18661 atttttaata aatgggtgct ggggatgcag gatttgctgc accgtgtgca cggcggcaat
    18721 attaccaaat gggtactgat ggggggtgcg attggcggtc tgtgtggatt gctggggttt
    18781 gtggcaccag caacgtcggg cggcggtttt aacctgattc ctatcgctac cgcggggaat
    18841 ttcagcatgg gaatgctggt gtttatcttc gtcgctcggg tcattaccac cttactctgc
    18901 ttctcttccg gcgcgccggg cggtattttt gccccgatgc tggcgctggg tactgtgctg
    18961 ggaaccgctt tcggaatggt tgccgttgag ctgtttccgc aatatcacct ggaggcgggg
    19021 acgtttgcta ttgccggaat gggggcatta ctggcggcat ctattcgcgc gccgttaacg
    19081 gggatcattc tggttctgga gatgaccgat aactaccagc tcattttgcc aatgattatt
    19141 accggtcttg gcgcaacact attagcgcaa tttaccggcg ggaaacctct atactcggcg
    19201 attcttgcgc gcacgctggc aaaacaggaa gctgagcaac tggcgcgaag caaggccgca
    19261 tcagccagcg agaatacttg aacgaaatac cagggtatta gataatggcg attattattg
    19321 ggttagaatt tgcccgattg ccgatgtcgt ttggagcaaa atatgagtga tgacgtagca
    19381 ctgccgctgg agtttaccga cgcagcagcc aacaaagtta aaagcctgat cgctgacgaa
    19441 gataacccga atctgaaatt acgcgtgtat atcaccggtg gcggttgcag cggcttccag
    19501 tatggtttca cctttgatga tcaggtgaac gaaggcgata tgaccatcga aaaacagggc
    19561 gttggcctgg tggttgatcc gatgagcctg caatatctgg tcggcggttc cgttgactac
    19621 accgaaggac tggaaggttc tcgtttcatc gtgaccaacc cgaacgcgaa aagcacctgc
    19681 ggttgcggtt cttcctttag catctaatct gttgttcatg tgccggataa actatccggc
    19741 acatcttctc tcttcaacgc ccattctcat ccagcgcaaa cgtcggtagc ttaagatgcc
    19801 aacgaatagc cgccagccga atcaatagcg tcacgaccat gcccatcata ctggctgttt
    19861 ccagtggtac ggaaaatgtg taataagccg tagcgtggac aataccgccg ataatacagg
    19921 cagttgcgta gatttctgta cgtaaaatca tggggatttc gcgggccagt acatcacgaa
    19981 tgatcccgcc gccaacgcca gtaatgacgc ccatacaaac cgcgattaac ggaccggctt
    20041 ccgcattaaa ggctttattc acgccaatgc cgacaaacac cgccagacca acggcgtcca
    20101 gcaccggcaa catccatttt ggtaagcgtc ttggctggcg caccagcacg atggtcagca
    20161 tgctggtgac catggcaacg accagatcgg tgggatcttt cacccaaaat accgggccgt
    20221 gatccagcgc catgtcgcga attgttccgt cgcctactgc ggtaaccacg cccagtacca
    20281 gaacaccaaa agggtccata cgcaattttc cggctaacaa aacgccggag atggcaaata
    20341 ccgctgtgcc gactatatcc agccaataga cgagcattgt tcaatcccca ctgagcacct
    20401 gaaaaggtca ggcactaatc tacctgtgaa agcgcattac agagctgttg tgctgcgagg
    20461 ataatacgtg ggcttgcacg ttcaaaccag tcactcgtga gaggaataac gggaattttg
    20521 agctgttcac cccagtattg tttgatttta ggaatttggt ccggtccgcc tgtaatgaca
    20581 atcgcctgtg gcgagcgtgc taacacctgt tcgcggctaa cttgcggcca gggaacccgg
    20641 ctgtctttaa agatgttttc tccgccacaa acttcgagta cctggttctg aatcgactct
    20701 tttccactgg taaatggcgg attaatgccg aattgcagaa aaacacgttt tttaggttta
    20761 tcagcatatt gcgctttcaa ttgcacgtac tgatccagca gggattgcgc ggcttgttcg
    20821 gccttgtctg gttgcggact ccagggggcc agttgacgta acgcattggc aatttgttca
    20881 atgcttgtcg catcgaccca catcactttt attcccagcg aagccagctg gtcaacctgc
    20941 cgctcggcat tacctccacg ccaggcaatc accagatcgg gtttcagcgc gacaatgcgt
    21001 tccagattca tcccctgcca ggtggaaacc tgctcaatct tttgcgcttg tggaggatag
    21061 tcggaatagc tgctgacccc aaccggcgtg atcccggcgg caaaggcaag ttcagtgttg
    21121 gcgggagaaa gcgtgatgac gcgcggcgcg gcgttgagcc acagtggcgc gagaaaagac
    21181 agggcgacca gcgccctgaa cagtgattta gccatgtgcc agtttctgca ccagtgactc
    21241 aaccatcagg ctggactgtt tagcggcaac agccaggaac tcatcgaagc taagatgaga
    21301 ctgttgatcg gccacgtcgg agatggcgcg tacgacaaca aacgggatgt tgaaattgtg
    21361 gcagacatgg gcgattgccg tcgcttccat ctctacagca atggcctgtg ggaagttgtg
    21421 gcggattttc gccagaccaa cagaaccgtt gatgaaagcg tcgccgctaa caatcaggcc
    21481 acgtacagcg ttaagattca gttcggcaat gcaggcctca gcggcagcga tcagtttatc
    21541 gtcagcttta aagcctgccg gacagcctgg taactgaccg tattcataac caaatgccgt
    21601 gacatccgcg tcgtgataac gcgcttcgtc cgagacaacg atatcgccca ctttcagcgt
    21661 tggtgccagg caaccggcag aaccggtgtt aataatcaca tctggcttgc agtgttccaa
    21721 cagcagagtg gcacccagcg ccgcagcgac tttaccgatg cccgatttca gaagcgcaac
    21781 ctcggttcca ttcagttggc cggtatagat ttcgcaaccg ccgagactga tagtttgacg
    21841 gttttcgatt ttgtcacgca gcagcgtaac ttcttcttcc attgcaccaa tgatgccgat
    21901 tttcatagat ttactcgcga taagcccgat ttgaaggcat agtttaccat gcgcttacgg
    21961 ggaagcgtat ttctcacgcg ggagaggaca tggcacagat tgatttccga aaaaaaataa
    22021 actggcatcg tcgttaccgt tcaccgcagg gcgttaaaac cgaacatgag atcctgcgga
    22081 tcttcgagag cgatcgcggg cgtatcatca actctccggc aattcgtcgt ctgcaacaaa
    22141 agacccaggt ttttccactg gagcgcaatg ccgccgtgcg cacgcgtctt acccactcga
    22201 tggaagtcca gcaggtggga cgctacatcg ccaaagaaat tttaagccgt ctgaaagagc
    22261 ttaaattgct ggaagcatac ggcctggatg aactgaccgg cccctttgaa agcattgttg
    22321 agatgtcatg cctgatgcac gatatcggca atccgccgtt tggtcatttt ggcgaagcag
    22381 cgataaatga ctggtttcgc caacgtttgc acccggaaga tgccgaaagc cagcctctga
    22441 ctgacgatcg ctgcagcgtg gcggcactac gtttacggga cggggaagaa ccgcttaacg
    22501 agctgcggcg caagattcgt caggacttat gtcattttga ggggaatgca caaggcattc
    22561 gtctggtgca tacattgatg cggataaatc tcacttgggc acaggttggc ggtattttaa
    22621 aatatacccg tccggcgtgg tggcgtggcg aaacgcctga gacacatcac tatttaatga
    22681 aaaagccggg ttattatctt tctgaagaag cctatattgc ccggttgcgt aaagaactta
    22741 atttggcgct ttacagtcgt tttccattaa cgtggattat ggaagctgcc gacgacatct
    22801 cctattgtgt ggcagacctt gaagatgcgg tagagaaaag aatatttacc gttgagcagc
    22861 tttatcatca tttgcacgaa gcgtggggcc agcatgagaa aggttcgctc ttttcgctgg
    22921 tggttgaaaa tgcctgggaa aaatcacgct caaatagttt aagccgcagt acggaagatc
    22981 agttttttat gtatttacgg gtaaacaccc taaataaact ggtaccctac gcggcacaac
    23041 gatttattga taatctgcct gcgattttcg ccggaacgtt taatcatgca ttattggaag
    23101 atgccagcga atgcagcgat cttcttaagc tatataaaaa tgtcgctgta aaacatgtgt
    23161 ttagccatcc ggatgtcgag cagcttgaat tgcagggcta tcgggtcatt agcggattat
    23221 tagagattta tcgtccttta ttaagcctgt cgttatcaga ctttactgaa ctggtagaaa
    23281 aagaacgggt gaaacgtttc cctattgagt cgcgcttatt ccacaaactc tcgacgcgcc
    23341 atcggctggc ctatgtcgag gctgtcagta aattaccgtc agattctcct gagtttccgc
    23401 tatgggaata ttattaccgt tgccgcctgc tgcaggatta tatcagcggt atgaccgacc
    23461 tctatgcgtg ggatgaatac cgacgtctga tggccgtaga acaataacca ggcttttgta
    23521 aagacgaaca ataaattttt accttttgca gaaactttag ttcggaactt cgggctataa
    23581 aacgaatctg aagaacacag caattttgcg ttatctgtta atcgagactg aaatacatga
    23641 aaaaaaccac attagcactg agtgcactgg ctctgagttt aggtttggcg ttatctccgc
    23701 tctctgcaac ggcggctgag acttcttcag caacgacagc ccagcagatg ccaagccttg
    23761 caccgatgct cgaaaaggtg atgccttcag tggtcagcat taacgtagaa ggtagcacaa
    23821 ccgttaatac gccgcgtatg ccgcgtaatt tccagcagtt cttcggtgat gattctccgt
    23881 tctgccagga aggttctccg ttccagagct ctccgttctg ccaggggggc cagggcggta
    23941 atggcggcgg ccagcaacag aaattcatgg cgctgggttc cggcgtcatc attgatgccg
    24001 ataaaggcta tgtcgtcacc aacaaccacg ttgttgataa cgcgacggtg attaaagtcc
    24061 agctgagcga tggtcgtaag ttcgacgcga agatggttgg caaagatccg cgctctgata
    24121 tcgcgctgat ccaaatccag aacccgaaaa acctgaccgc aattaagatg gcggattctg
    24181 atgcactgcg cgtgggtgat tacaccgtag cgattggtaa cccgtttggt ctgggcgaga
    24241 cggtaacttc cgggattgtc tctgcgctgg ggcgtagcgg cctgaatgct gaaaactacg
    24301 aaaacttcat ccagaccgat gcagcgatca accgtggtaa ctccggtggt gcgctggtta
    24361 acctgaacgg cgaactgatc ggtatcaaca ccgcgatcct cgcaccggac ggcggcaaca
    24421 tcggtatcgg ttttgctatc ccgagtaaca tggtgaaaaa cctgacctcg cagatggtgg
    24481 aatacggcca ggtgaaacgc ggtgagctgg gtattatggg gactgagctg aattccgaac
    24541 tggcgaaagc gatgaaagtt gacgcccagc gcggtgcttt cgtaagccag gttctgccta
    24601 attcctccgc tgcaaaagcg ggcattaaag cgggtgatgt gatcacctca ctgaacggta
    24661 agccgatcag cagctttgcc gcactgcgtg ctcaggtggg tactatgccg gtgggcagca
    24721 aactgaccct gggcttactg cgcgacggta agcaggttaa cgtgaacctg gaactgcagc
    24781 agagcagcca gaatcaggtt gattccagct ccatcttcaa cggcattgaa ggcgctgaga
    24841 tgagcaacaa aggcaaagat cagggcgtgg tagtgaacaa cgtgaagacg ggcactccgg
    24901 ctgcgcagat cggcctgaag aaaggtgatg tgattattgg tgcgaaccag caggcagtga
    24961 aaaacatcgc tgaactgcgt aaagttctcg acagcaaacc gtctgtgctg gcactgaaca
    25021 ttcagcgcgg cgacagcacc atctacctgt taatgcagta atctccctca accccttcct
    25081 gaatacggga aggggttctc cttacaatct gtgaacttcg ccacaactcc atacatcttc
    25141 atcatccttt gggcatttgc acaatgccgt acgttacgta cttccttatg ctaagccgtg
    25201 cataacggag gacttatggc tggctggcat cttgatacca aaatggcgca ggatatcgtg
    25261 gcacgtacca tgcgcatcat cgataccaat atcaacgtaa tggatgcccg cgggcgaatt
    25321 atcggcagcg gcgatcgtga gcgtattggt gaattgcacg aaggtgcatt gctggtactt
    25381 tcacagggac gagtcgtcga tatcgatgac gcggtagcac gtcatctgca cggtgtgcgg
    25441 caggggatta atctaccgtt acggctggaa ggtgaaattg tcggcgtaat tggcctgaca
    25501 ggtgaaccag agaatctgcg taaatatggc gaactggtct gcatgacggc tgaaatgatg
    25561 ctggaacagt cgcggttgat gcacttgttg gcgcaggata gccgtttgcg ggaagaactg
    25621 gtgatgaacc tgattcaggc agaggagaat actcccgcac ttctgaatgg gcgcaacggc
    25681 tggggatcga tctcaatcaa ccgcgagtgg tggctattgt tgaggtcgac agcggtcagc
    25741 ttggcgtgga cagcgcaatg gcggagttac aacaactgca aaacgcgctg actacgcccg
    25801 agcgtaataa tctggtggcg attgtctcgc taaccgaaat ggtggtgttg aaaccggcgt
    25861 tgaactcttt tgggcgctgg gatgcagaag atcatcgtaa gcgagttgaa caactgatta
    25921 cccgcatgaa agagtacggc cagctgcgtt ttcgcgtttc actgggcaac tattttaccg
    25981 gtcctggcag tattgcccga tcctatcgta cggcgaaaac gacgatggtg gtgggtaaac
    26041 agcggatgcc agaaagtcgc tgctattttt atcaggatct gatgttacct gtgttactcg
    26101 acagtttgcg tggcgactgg caggccaacg aactggcgcg accgctggcg cggctgaaag
    26161 cgatggacaa taacggcttg ctgcgacgaa cgctggcggc gtggtttcgc cacaatgtgc
    26221 aaccgctggc aacgtcaaag gcgttgttta ttcatcgtaa taccctggag tatcggctta
    26281 atcgtatatc ggaactgacc gggcttgatt tgggcaattt tgatgacagg ttgctgctgt
    26341 atgtggcgtt acaactggat gaagagcggt agattatgcg ttaaggttga ggcggcgctg
    26401 gctcattccc ctcagcccga aggggagagg ggaatgcccg agttaaccgt caattcggga
    26461 ttatttacgc gttagttttt ccagatcggc ttcaatctcg ctgatcttat tggttacgac
    26521 tgactccagg tgacgtaagt cgtcgaggat cttacgctta agatcaactt cgctgcggtc
    26581 acgctgacag atttgatcaa gctcatcaat gatataccgt agattcgggc tgatttcctg
    26641 gacttcttta taaccctgac ccacaccatc agcgacgacc gttttacgct gacgcggata
    26701 tttaaactta acgctcttgg cgaaaaactc gcctttgtct ttctggaaat agattttcag
    26761 aatatcgttg ttggcttcct gccggaggct gtaacgatca atttcttcag gattggtaat
    26821 acccagactt ttcagattgt cgtacatagc gttacctcaa aatgagtcag taaattgtgc
    26881 ttattttagc atttggcttg gcccgcccgg ctcgactgtg atcgcagaaa gccgttttct
    26941 tccagacttc tatcatggcg taaaaatcaa aaaattacct gctttattgt ggcgatattc
    27001 catgggatgg attagcacat ttatcttcgt tttgcgaatt acatgacagt ccgaaacaag
    27061 atgaaaaagt atttaaaaaa gggggaggaa gaatgagtag gacgaattat tctgagccag
    27121 aaagcatacg ggcagcatca tgctgcccgt atatgtactc ttaatcgatg gtacgcagca
    27181 gttcgttaat gccgactttg ccgcgagttt tcgcgtcaac tttcttaacg ataaccgcac
    27241 agtagaggct gtatttgcca tcttttgacg gcagattacc tgaaacaacc acagaccccg
    27301 ccggaacgcg accgtagtgg atttcgccgg tttcacggtc gtaaatacgg gtgctctgac
    27361 caatgtatac gcccatagag atgacggaac cttcttcgac aatcacccct tcaaccactt
    27421 cagagcgtgc gccaatgaag cagttatctt caataatggt cgggttagcc tgcagcggct
    27481 ccagcacgcc gccgatgccc acgccaccgg aaaggtggac gtttttacca atctgcgcac
    27541 aagaaccgac ggtcgcccag gtatcaacca tggtgccttc atcaacatat gcgccgatgt
    27601 taacgtaaga cggcatcagc acggtgttac gggcaataaa cgcaccctga cgtaccgccg
    27661 ctggtggcac aacgcggaag ccttctttct ggaaacgtgc ttcgtcgtag tcggcgaatt
    27721 tcatcggcac tttgtcgaag tagcggcttt ctgccccttc gatcacctga ttatcattaa
    27781 tacggaaaga gagcagcacc gcttttttca accactgatg cgtcacccac tgaccgtcaa
    27841 ttttttccgc tacacgcagt gcgccggaat ccagcagggc gatcacctga tttaccgctt
    27901 cgcgggtaac ggtgtctgca ttggctggcg tgatctcggc acggcgttca aaagcggttt
    27961 caataatgtt ctgtaactgc tgcattgtta aactcttttc atatcagtaa acacatcacc
    28021 ctttatcgtt tggattgagg gcctctgtca accgctgatg cacttcctgc tgcaactcgt
    28081 tattaagcgc acgccggtca gcggtggcaa ttatgaataa atcttctact cgctcgccaa
    28141 tggttgtaat tcgggcacca tgaagcgaaa ttcccagatc ggcaaaaatt ttcccgactc
    28201 gcgccagcag tccaggttgg tcgagggcga tcagttcgag gaacgatttg cggtcggtat
    28261 gggtcggcaa aaacgttact tcggtttcaa cagtaaaatg gcgtaatttg gcgggttggc
    28321 gacggggctg cggtggctgc cagctacttt gcgtcagcac ctgctccaga ccaaaccgaa
    28381 taacctcatg gcgatctgcg gacagcgggc tgccatcggg ttccagcacg ataaaggtat
    28441 ccatcgccat accgtcgcga gtggtgaaaa tttgtgcgtc gtgaacactt aaattgcggc
    28501 ggtctaattc ggcacagacg gcggcaaaca gataggggcg gtccgggctc cagataaaaa
    28561 tctcggtgcc tccacgcgta gcctgcgggc taagcaatac cagcggtttg cttaaatcat
    28621 gctgtaataa atggcgggca tgccaggcca gttgatttgg gctatggcgg acaaaatagt
    28681 tagcacgaca gcgtgaccaa atttggtgca gcgcctcttc gtcgatgtta tccatgcgca
    28741 gtagtgccag tgcctggagt tggtgatggc gaacccgttc gcgcatatcc ggcgtgtttt
    28801 gcattccgcg tcgtagctgc ttttcggtgg caaaatagag ttcgcgcaac agactttgct
    28861 tccagctatt ccacagcgtt tcgttggtgg cgcaaatgtc agccactgtc aggcatacca
    28921 gatagcgcag acgattttcc gtttgaactt cttcggcaaa ctgcttgatg acttcagggt
    28981 cctgaatatc gcggcgttgt gctgtcaccg acatcagcaa gtgctggcga accagccagg
    29041 caaccagctg tgtttcgcgt gagttcagcc cgtggagttc tgcaaaatgc actacatcct
    29101 gagcaccgag aatggagtgg tcgccgccgc gtcctttggc gatatcgtga aacagcgcgg
    29161 cgatgaaaat cagctcagtt gacggcaggc gcggccagac gtccacacac aacggatggc
    29221 gctggcgcgt ttcttcactg gcaaaactct ccagtttcag catcacgcgg atagtatgtt
    29281 catccaccgt gtaggcgtgg aacagatcaa actgcatctg cccgacgata tgcgaccatt
    29341 gcggcatata cgcgccgagc acgctatggc gatgcattgg caatagcccg cgccgcaccg
    29401 ctccggggtg acgcagaatg ctcaaaaaca gttttcgtgc ttgcggaata ttacacagcg
    29461 gttgttgcag atggcggcgg gcatggcgta actggcgcag cgtggtggag taaatgccgg
    29521 tgatcgcact gttgcgcacc atggtgtaga acatacgcaa gatggcttcc ggctggcgca
    29581 taaatagtgt ttcatcacgc aggtcgatta gcgtaccgcg tagctgaaac tcatcgtcga
    29641 gtggacgtgg tttttcgtcg gcgggaaggg cgaggatggc ttcatcgaac agttgcagca
    29701 gcatctggtt gagttcactg acgcggcgtg taacgcggaa gtaatccttc atcatccgct
    29761 cgaccggttc gttgccttca ccactgtaat tcagacgctg agcgacgcta agttggcgat
    29821 cgaataacag acgattatcg taacggctga cgaccagatg cagggcaaag cgaatacgcc
    29881 acagtatatg cagacattcg tttaattccg cccgctccgc tgaggttaag aagccaaacc
    29941 cgaccatttc atccagcgat gttgcgccaa aatgacggcg ggccacccat tgcagagtgt
    30001 gaatatcgcg caagccgcca gggctacttt tgatgtctgg ttcaaggttg tagctggtgc
    30061 catggtaacg ctgatggcgc tggttctgtt cttcaacttt cgctgcgtag aacttgtcgg
    30121 aaggccagaa tccttcgcta aaaatatgtt tttgcagttc gaggaagagc gcaacatcgc
    30181 caattaataa gcgggattcg attaaattgg tggcgacggt taaatccgat aacccttcca
    30241 gcatgcactc ttcaagcgtg cgcacactat gaccgacttc cagctttaca tcccagagca
    30301 gcgttaacag ctcgcccact ttttgcgcct gatcgtccgg gagcttttta cggcttaaaa
    30361 tcagtaaatc gatgtcagaa agtggatgca gctcgccacg accgtagcca ccgacggcaa
    30421 ccaatgccag gtcggcaatc tggctgaatc ccgcttcaat ccataatcgt tgcaggagct
    30481 ggtctatgaa ctcggtacgt gcctcaatca actgttctgc agagatccca ttgtcaaagg
    30541 catcacccag ccaacgctgg aaagtatcga tatgggcttt tatcccacag acggttaatt
    30601 catcacgggg ccaggcgcat ggattttgcg gttgaccggg cagggtgggg agagcggtgt
    30661 ttgcgtactg ttctggaagg gtattcattg tgcgccaccc ataagattaa attatcgcat
    30721 taaaaaagcc ggcattcgcc ggcttcatct tattcgtcgt gcgagattat cgccgggatg
    30781 gtgtcatcct tgcgtagcgt caaaatttcg cagccgttat cagtcaccac aatagtatgc
    30841 tcatattgtg cagacaagct gcgatctttg gtttttaccg tccagccatc tttcatggtg
    30901 cggatctctt ttttacccgc gttgaccatt ggctcaatgg tgaacgtcat ccccggtttc
    30961 agcacgacgt tggtttcacg ggagtcatag tgcagcacct gcggttcttc atggaagccg
    31021 cgaccaatac cgtgtccgca atattcacga acgacggaga agccttctgc ttcgacaaat
    31081 ttctgaatcg ccgcaccgat ttcgcgcaga ttaatgcctg gttttaccat gcgtagcgcc
    31141 aggtacaggc tttcttgcgt gatgcggcac aggcgttcgc ccatgatggt cggcttaccg
    31201 acgataaaca ttttcgaggt atcgccgtgg aaaccatctt tgattacggt gacatcaatg
    31261 ttaacgatat cgccatcttt cagcagctta gcatcgtccg ggataccgtg gcacaccact
    31321 tcattaatag agatgcaaac ggatttcgga tagccgtgat agccgaggca ggcagaaacc
    31381 gcgtgttgtt cattaacaat gtaatcatta cagatgcgat ccagctcgcc ggtgctgacg
    31441 cccggtttaa catacggttc gatcatctcc agcacttcgg cagccagtcg gccagcgacg
    31501 cgcatttttt cgatatcttc tggggtcttg attgagatag ccattaattc tgtccatcag
    31561 cgtcggtgat accgacaata tatatgtaag tgccgtcaat ggtatcacac ccgggcaaat
    31621 tgagaatcat tctgaatttc gccaaacgtg ccactgaagg ttttctataa tagaaaattc
    31681 gacgtctgat gctgtacaca gcgccaacag ttattggtgt ccacgacgta tttgtggtat
    31741 aaagcgcgcc ggacttccga tccatttcgt atacacagac tggacggaag cgacaatctc
    31801 actttgtgta acaacacaca cgtatcggca catattccgg ggtgcccttt ggggtcggta
    31861 atatgggata cgtggaggca taaccccaac ttttatatag aggttttaat catggcaact
    31921 gtttccatgc gcgacatgct caaggctggt gttcacttcg gtcaccagac ccgttactgg
    31981 aacccgaaaa tgaagccgtt catcttcggt gcgcgtaaca aagttcacat catcaacctt
    32041 gagaaaactg taccgatgtt caacgaagct ctggctgaac tgaacaagat tgtttctcgc
    32101 aaaggtaaaa tccttttcgt tggtactaaa cgcgctgcaa gcgaagcggt gaaagacgct
    32161 gctctgagct gcgaccagtt cttcgtgaac catcgctggc tgggcggtat gctgactaac
    32221 tggaaaaccg ttcgtcagtc catcaaacgt ctgaaagacc tggaaactca gtctcaggac
    32281 ggtactttcg acaagctgac caagaaagaa gcgctgatgc gcactcgtga gctggagaaa
    32341 ctggaaaaca gcctgggcgg tatcaaagac atgggcggtc tgccggacgc tctgtttgta
    32401 atcgatgctg accacgaaca cattgctatc aaagaagcaa acaacttggg tattccggta
    32461 tttgctatcg ttgataccaa ctctgatccg gacggtgttg acttcgttat cccgggtaac
    32521 gacgacgcaa tccgtgctgt gaccctgtac ctgggcgctg ttgctgcaac cgtacgtgaa
    32581 ggccgttctc aggatctggc ttcccaggcg gaagaaagct tcgtagaagc tgagtaataa
    32641 ggcttgataa ctcccccaaa atagttcgag ttgcagaaag gcggcaagct cgagaattcc
    32701 cgggagctta catcagtaag tgaccgggat gagcgagcga agacaacgca tctgcggcgc
    32761 gaaatatgaa gggggagagc ccttatagac caggtagtac acgtttggtt agggggcctg
    32821 catatggccc cctttttcac ttttatatct gtgcggttta atgccgggca gatcacatct
    32881 ccgaggattt tagaatggct gaaattaccg catccctggt aaaagagctg cgtgagcgta
    32941 ctggcgcagg catgatggat tgcaaaaaag cactgactga agctaacggc gacatcgagc
    33001 tggcaatcga aaacatgcgt aagtccggtg ctattaaagc agcgaaaaaa gcaggcaacg
    33061 ttgctgctga cggcgtgatc aaaaccaaaa tcgacggcaa ctacggcatc attctggaag
    33121 ttaactgcca gactgacttc gttgcaaaag acgctggttt ccaggcgttc gcagacaaag
    33181 ttctggacgc agctgttgct ggcaaaatca ctgacgttga agttctgaaa gcacagttcg
    33241 aagaagaacg tgttgcgctg gtagcgaaaa ttggtgaaaa catcaacatt cgccgcgttg
    33301 ctgcgctgga aggcgacgtt ctgggttctt atcagcacgg tgcgcgtatc ggcgttctgg
    33361 ttgctgctaa aggcgctgac gaagagctgg ttaaacacat cgctatgcac gttgctgcaa
    33421 gcaagccaga attcatcaaa ccagaagacg tatccgctga agtggtagaa aaagaatacc
    33481 aggtacagct ggatatcgcg atgcagtctg gtaagccgaa agaaatcgca gagaaaatgg
    33541 ttgaaggccg catgaagaaa ttcaccggcg aagtttctct gaccggtcag ccgttcgtta
    33601 tggaaccaag caaaactgtt ggtcagctgc tgaaagagca taacgctgaa gtgactggct
    33661 tcatccgttt cgaagtgggt gaaggcatcg agaaagttga gactgacttt gcagcagaag
    33721 ttgctgcgat gtccaagcag tcttaattat caaaaaggag ccgcctgagg gcggcttctt
    33781 tttgtgccca tcttgtaaat tcagctaacc cttgtggggc tgcgctgaaa agcgacgtac
    33841 aatgtcgcta gtattaattc atttcaatcg ttgacagtct caggaaagaa acatggctac
    33901 caatgcaaaa cccgtctata aacgcattct gcttaagttg agtggcgaag ctctgcaggg
    33961 cactgaaggc ttcggtattg atgcaagcat actggatcgt atggctcagg aaatcaaaga
    34021 actggttgaa ctgggtattc aggttggtgt ggtgattggt gggggtaacc tgttccgtgg
    34081 cgctggtctg gcgaaagcgg gtatgaaccg cgttgtgggc gaccacatgg ggatgctggc
    34141 gaccgtaatg aacggcttgg caatgcgtga tgcactgcac cgcgcctatg tgaacgctcg
    34201 tctgatgtcc gctattccat tgaatggcgt gtgcgacagc tacagctggg cagaagctat
    34261 cagcctgttg cgcaacaacc gtgtggtgat cctctccgcc ggtacaggta acccgttctt
    34321 taccaccgac tcagcagctt gcctgcgtgg tatcgaaatt gaagccgatg tggtgctgaa
    34381 agcaaccaaa gttgacggcg tgtttaccgc tgatccggcg aaagatccaa ccgcaaccat
    34441 gtacgagcaa ctgacttaca gcgaagtgct ggaaaaagag ctgaaagtca tggacctggc
    34501 ggccttcacg ctggctcgtg accataaatt accgattcgt gttttcaata tgaacaaacc
    34561 gggtgcgctg cgccgtgtgg taatgggtga aaaagaaggg actttaatca cggaataatt
    34621 cccgtgatgg ataaataagg gtaagattcc gcgtaagtat cgcgggggcg taagtctggt
    34681 tataaggcgt tattgttgca ggcagtttgg tcacggccag cgcgcagcaa ccggagcgta
    34741 caaaagtacg tgaggatggc gagcactgct cggggccaaa atggcaaata aaatagccta
    34801 ataatccaga cgattacccg taatatgttt aatcagggct atacttagca cacttccact
    34861 gtgtgtgact gtctggtctg actgagacaa gttttcaagg attcgtaacg tgattagcga
    34921 tatcagaaaa gatgctgaag tacgcatgga caaatgcgta gaagcgttca aaacccaaat
    34981 cagcaaaata cgcacgggtc gtgcttctcc cagcctgctg gatggcattg tcgtggaata
    35041 ttacggcacg ccgacgccgc tgcgtcagct ggcaagcgta acggtagaag attcccgtac
    35101 tctgaaaatc aacgtgtttg atcgttcaat gtctccggcc gttgaaaaag cgattatggc
    35161 ctccgatctc ggcctgaacc cgaactctgc gggtagcgac atccgtgttc cgctgccgcc
    35221 gctgacggaa gaacgtcgta aagatctgac caaaatcgtt cgtggtgaag cagaacaagc
    35281 gcgtgttgca gtacgtaatg tgcgtcgtga cgcgaacgac aaagtgaaag cactgttgaa
    35341 agataaagag atcagcgaag acgacgatcg ccgttctcag gacgatgtac agaaactgac
    35401 tgatgctgca atcaagaaaa ttgaagcggc gctggcagac aaagaagcag aactgatgca
    35461 gttctgattt cttgaacgac aaaaacgccg ctcagtagat ccttgcggat cggctggcgg
    35521 cgttttgctt tttattctgt ctcaactctg gatgtttcat gaagcaactc accattctgg
    35581 gctcgaccgg ctcgattggt tgcagcacgc tggacgtggt gcgccataat cccgaacact
    35641 tccgcgtagt tgcgctggtg gcaggcaaaa atgtcactcg catggtagaa cagtgcttgg
    35701 aattctctcc ccgctatgcc gtaatggacg atgaagcgag tgcgaaactt cttaaaacga
    35761 tgctacagca acaggggagc cgcaccgaag tcttaagtgg gcaacaagcc gcttgcgata
    35821 tggcagcgct tgaggaggtt gatcaggtga tggcagccat tgttggtgct gctggactgt
    35881 tacctacgct tgctgcgatc cgcgcgggta aaaccatttt gctggccaat aaagaatcac
    35941 tggttacctg cggacgtctg tttatggacg ccgtaaagca gagcaaagcg caattgttac
    36001 cggtcgatag cgaacataac gctatttttc agagtttacc gcaacctatc cagcataatc
    36061 tgggatacgc tgaccttgag caaaatggcg tggtgtccat tttacttacc gggtctggtg
    36121 gccctttccg tgagacgcca ttgcgcgatt tggcaacaat gacgcccgat caagcttgcc
    36181 gtcatccgaa ctggtcgatg ggacgtaaaa tttctgtcga ttcggctacc atgatgaaca
    36241 aaggtctgga atacattgaa gcgcgttggc tgtttaacgc cagcgccagc cagatggaag
    36301 tgctgattca cccgcagtca gtgattcact caatggtgcg ctatcaggac ggcagtgttc
    36361 tggcgcagct gggggaaccg gatatgcgta cgccaattgc ccacaccatg gcatggccga
    36421 atcgcgtgaa ctctggcgtg aagccgctcg atttttgcaa actaagtgcg ttgacatttg
    36481 ccgcaccgga ttatgagcgt tatccatgcc tgaaactggc gatggaggcg ttcgaacaag
    36541 gccaggcagc gacgacagca ttgaatgccg caaacgaaat caccgttgct gcttttcttg
    36601 cgcaacaaat ccgctttacg gatatcgctg cgttgaattt atccgtactg gaaaaaatgg
    36661 atatgcgcga accacaatgt gtggacgatg tgttatctgt tgatgcgaac gcgcgtgaag
    36721 tcgccagaaa agaggtgatg cgtctcgcaa gctgaggata atccggctac agagagtcgc
    36781 gctatttgtt agcgtagggc ttcagtgata tagtctgcgc catctgatcg taagtagttg
    36841 gctttataag gtcaggtatg ccgtggtttt acacggcttt tttttgtata ggcttcagta
    36901 ttcctgagta ccgtaaaccc tgtcagggaa taaaaaacgc gtgatgttgt ctgctactca
    36961 accacttagc gaaaaattgc cagcgcatgg ctgccgtcat gttgcgatca ttatggacgg
    37021 caatggtcgc tgggcaaaaa agcaagggaa gattcgtgcc tttgggcata aagccggggc
    37081 aaaatccgtc cgccgggctg tctcttttgc ggcaaacaac ggtattgagg cgttaacgct
    37141 gtatgccttt agtagtgaaa actggaaccg accagcgcag gaagtcagtg cgttaatgga
    37201 actgtttgtg tgggcgctcg atagcgaagt aaaaagtctg caccgacata acgtgcgtct
    37261 gcgtattatt ggcgatacca gtcgttttaa ctcgcgtttg caagaacgta ttcgtaaatc
    37321 tgaagcgcta acagccggaa ataccggtct gacgctgaat attgcggcga actacggtgg
    37381 acgttgggat atagtccagg gagtcaggca actggctgaa aaggtgcagc aaggaaacct
    37441 gcaaccagat cagatagatg aagagatgtt aaaccagcat gtctgtatgc atgaactggc
    37501 ccctgtagat ttagtaatta ggactggggg agagcatcgc attagtaact ttttgctttg
    37561 gcaaattgcc tatgccgaac tttactttac agatgttctc tggcccgatt tcgatgaaca
    37621 agactttgaa ggggcgttaa atgcctttgc taatcgagag cgtcgtttcg gcggcaccga
    37681 gcccggtgat gaaacagcct gatgggggtc gcttttgctg aagtatcgcc tgatatctgc
    37741 ttttgtgtta atacccgtcg tcatcgcggc gttgtttctg ttgccgccgg tggggttcgc
    37801 cattgtaacg ctggtggtct gcatgctggc agcgtgggaa tggggacagc ttagcggttt
    37861 taccactcgt tcgcagcgag tatggttggc ggtgttatgc gggttattgt tggcgctgat
    37921 gctttttctg ttgccggaat atcaccgaaa tattcatcaa ccgctggttg aaatctcact
    37981 ttgggcttcg ttgggttggt ggattgtcgc gctattgctg gtgctgtttt acccaggttc
    38041 cgcagcaatc tggcgtaact ctaaaacatt gcgccttatt tttggcgtgc taaccattgt
    38101 tcccttcttc tggggcatgc tggcgttacg ggcctggcac tatgacgaga atcattacag
    38161 tggcgcaata tggctgctct atgtcatgat cctggtatgg ggcgctgact ccggcgcata
    38221 tatgtttggc aaattgtttg gtaaacataa gctggcaccg aaggtttctc cgggtaaaac
    38281 ctggcaaggc tttataggtg gactcgctac tgcagcggta atctcatggg gttatggcat
    38341 gtgggcgaat ctcgacgtcg ctcccgtcac cttactcatt tgctctattg tcgcagcgtt
    38401 agcctcagtg ctcggcgatc tgaccgagag tatgtttaag cgtgaagcag gaattaagga
    38461 cagcggtcat ttaattccag gacacggtgg tattttagat cgtattgata gcctgacggc
    38521 tgcggtaccg gtctttgctt gcttgttgtt actggtattc aggacgcttt aacggaaggt
    38581 aatatgctga gttttctctg ggatttggct tcgttcatcg ttgcactggg tgtacttatc
    38641 accgtgcatg aatttggtca tttctgggtt gcccggcgtt gtggtgttcg cgttgagcgt
    38701 ttctcaatag ggtttggtaa ggcgctctgg cggcgaactg ataagctcgg caccgaatat
    38761 gttatcgccc tgatcccgtt gggcggttat gtcaaaatgc tggatgagcg cgcagaaccg
    38821 gtcgttccgg aactccgcca ccatgccttc aataataaat ctgtcggcca acgagcggcg
    38881 attattgccg caggtccggt tgcaaacttc atttttgcta tctttgccta ctggctggtt
    38941 tttattattg gtgtgcctgg cgtacgtccg gtggttggcg aaatagcagc caattcgata
    39001 gctgcggaag cacaaattgc accaggtacg gaactaaaag ccgtagatgg tatcgaaacg
    39061 cctgattggg atgccgtgcg tttgcagttg gtcgataaaa ttggcgatga aagcaccacc
    39121 attacagtag cgccatttgg cagcgaccaa cggcgggatg taaagctcga tttacgtcac
    39181 tgggcgtttg agcctgataa agaagatccg gtatcttcgc tggggattcg tcctcgtggg
    39241 ccgcaaattg aacctgtact ggaaaatgtg cagccaaact cggcggcaag caaggcaggt
    39301 ttgcaagcag gcgacaggat cgttaaagtc gatggtcagc ccttaacgca gtgggtgacc
    39361 tttgtgatgt ttgtccggga taacccgggt aaatccttag cgttagaaat cgaaaggcag
    39421 gggagtccct tgtctttgac attaatcccg gagagtaaac cgggtaatgg taaagcgatt
    39481 ggttttgtcg gtattgagcc gaaagtcatt cctttgccag atgagtataa agttgtacgc
    39541 cagtatgggc cgttcaacgc catcgtcgaa gccacggaca aaacgtggca gctgatgaag
    39601 ctgacggtca gtatgctggg aaaattgatc accggtgatg tgaaactgaa caacctcagt
    39661 gggccgatct ctatcgccaa gggggctggg atgacagcgg aactcggggt agtttattac
    39721 ctgccgtttc ttgcgcttat tagcgtgaac ttagggataa ttaacctgtt tccgttgccc
    39781 gtacttgacg gggggcatct gctgttcctt gcgatcgaaa agatcaaggg cggaccggta
    39841 tccgagcggg ttcaagactt ttgttatcgc attggctcga ttctgctggt gctgttaatg
    39901 gggcttgcac ttttcaatga tttctctcgg ttatgagagt tagttaggaa gaacgcataa
    39961 taacgatggc gatgaaaaag ttgctcatag cgtcgctgct gtttagcagc gccaccgtat
    40021 acggtgctga agggttcgta gtgaaagata ttcatttcga aggccttcag cgtgtcgccg
    40081 ttggtgcggc cctcctcagt atgccggtgc gtacaggcga cacggttaat gatgaagata
    40141 tcagtaatac cattcgcgct ctgtttgcta ccggcaactt tgaggatgtt cgcgtccttc
    40201 gtgatggtga tacccttctg gttcaggtaa aagaacgtcc gaccattgcc agcattactt
    40261 tctccggtaa caaatcggtg aaagatgaca tgctgaagca aaacctcgag gcttctggtg
    40321 tgcgtgtggg cgaatccctc gatcgcacca ccattgccga tatcgagaaa ggtctggaag
    40381 acttctacta cagcgtcggt aaatatagcg ccagcgttaa agctgtcgtg accccgctgc
    40441 cgcgcaaccg tgttgaccta aaactggtgt tccaggaagg tgtgtcagct gaaatccagc
    40501 aaattaacat tgttggtaac catgctttca ccaccgacga actgatctct catttccaac
    40561 tgcgtgacga agtgccgtgg tggaatgtgg taggcgatcg taaataccag aaacagaaac
    40621 tggcgggcga ccttgaaacc ctgcgcagct actatctgga ccgcggttat gcccgtttca
    40681 acatcgactc tacccaggtc agtctgacgc cagataaaaa aggtatttac gtcacggtga
    40741 acatcaccga aggcgatcag tacaagcttt ctggcgttga agtgagcggc aaccttgccg
    40801 ggcactccgc tgaaattgag cagctgacta agatcgagcc gggcgagctg tataacggca
    40861 ccaaagtgac caaaatggaa gatgacatta aaaagcttct cggtcgctat ggttatgcct
    40921 atccgcgcgt acagtcgatg cctgaaatta acgatgccga caaaaccgtt aaattacgcg
    40981 tgaacgttga tgcgggtaac cgtttctacg tgcgtaagat ccgttttgaa ggtaacgata
    41041 cctcgaaaga tgccgtcctg cgtcgcgaaa tgcgtcagat ggaaggtgca tggttgggga
    41101 gcgatctggt cgatcagggt aaggagcgtc tgaatcgtct gggcttcttt gaaactgtcg
    41161 ataccgatac ccaacgtgtt ccgggtagcc cggaccaggt tgatgtagtc tacaaggtga
    41221 aagaacgtaa cactggtagc ttcaacttcg ggattggtta cggtactgaa agtggcgtga
    41281 gcttccaggc tggcgtgcaa caggataact ggttaggtac aggttatgct gttggtatca
    41341 acgggaccaa aaacgattac cagacctatg ctgaactgtc ggtaaccaac ccgtacttca
    41401 ccgtagatgg cgtaagcctc ggtggtcgtc tcttctataa tgacttccag gcagatgacg
    41461 ccgacctgtc cgactatacc aacaagagtt atggtacaga cgtgacgttg ggcttcccga
    41521 ttaacgaata taactcgctg cgtgcaggtc tgggttatgt acataactcc ctgtccaaca
    41581 tgcagcctca ggttgcgatg tggcgttatc tgtactctat gggtgaacat ccgagcacct
    41641 ctgatcagga taacagcttc aaaacggacg acttcacgtt caactatggt tggacctata
    41701 acaagcttga ccgtggttac ttcccgacag atggttcacg tgtcaacctg accggtaaag
    41761 tgaccattcc tggatcggat aacgaatact acaaagtgac gttagacacg gcgacttatg
    41821 tgccgatcga tgacgatcac aaatgggttg ttctgggtcg tacccgctgg ggttatggtg
    41881 atggtttagg cggcaaagag atgccgttct acgagaactt ctatgccggt ggttccagca
    41941 ccgtgcgtgg cttccagtcc aataccattg gtccgaaagc agtttacttc ccgcatcagg
    42001 ccagtaatta tgatccggac tatgattacg aatgtgcgac tcaggacggc gcgaaagacc
    42061 tgtgtaaatc ggatgatgct gtaggcggta acgccatggc ggttgccagc ctcgagttca
    42121 tcaccccgac gccgtttatt agcgataagt atgctaactc ggttcgtact tcattcttct
    42181 gggatatggg taccgtttgg gatacaaact gggattccag ccaatattct ggatatccgg
    42241 actatagtga tccaagcaat atccgtatgt ctgcgggtat cgcattacaa tggatgtccc
    42301 cattggggcc gttggtgttc tcctacgccc agccgttcaa aaagtacgat ggagacaagg
    42361 cagaacagtt ccagtttaac atcggtaaaa cctggtaagt gttctccaca aaggaatgta
    42421 gtggtagtgt agcgatgact ttaggcgatc aatataagat cgccgggcca cgcaaagaac
    42481 tgcaccctcc ggtgcaaatg ggatggtaag gagtttattg tgaaaaagtg gttattagct
    42541 gcaggtcttg gtttagcact ggcaacttct gctcaggcgg ctgacaaaat tgcaatcgtc
    42601 aatatgggca gcctgttcca gcaggtagcg cagaaaaccg gtgtttctaa cacgctggaa
    42661 aatgagttca aaggccgtgc cagcgaactg cagcgtatgg aaaccgatct gcaggctaaa
    42721 atgaaaaaac tgcagtccat gaaagcgggc agcgatcgca ccaaactgga aaaagacgtg
    42781 atggctcagc gccagacttt tgctcagaaa gcgcaggctt ttgagcagga tcgcgcacgt
    42841 cgttccaacg aagaacgcgg caaactggtt actcgtatcc agactgctgt gaaatccgtt
    42901 gccaacagcc aggatatcga tctggttgtt gatgcaaacg ccgttgctta caacagcagc
    42961 gatgtaaaag acatcactgc cgacgtactg aaacaggtta aataagtaat gccttcaatt
    43021 cgactggctg atttagcgca gcagttggat gcagaactac acggtgatgg cgatatcgtc
    43081 atcaccggcg ttgcgtccat gcaatctgca caaacaggtc acattacgtt catggttaac
    43141 ccaaaatacc gtgagcattt aggcttgtgc caggcgtccg cggttgtcat gacccaggac
    43201 gatcttcctt tcgcgaaaag tgccgcgctg gtagtgaaga atccctacct gacttacgcg
    43261 cgcatggcgc aaattttaga taccacgccg cagcccgcgc agaacattgc acccagtgcg
    43321 gtgatcgacg cgacggcgaa gctgggtaac aacgtatcga ttggcgctaa cgcggtgatt
    43381 gagtccggcg ttgaactggg cgataacgtg attatcggtg ccggttgctt cgtaggtaaa
    43441 aacagcaaaa tcggtgcagg ttcgcgtctc tgggcgaacg taaccattta ccatgagatc
    43501 cagatcggtc agaattgcct gatccagtcc ggaacagtgg taggcgcaga cggctttggt
    43561 tatgccaacg atcgtggtaa ctgggtgaag atcccacaga ttggtcgcgt aattattggc
    43621 gatcgcgtgg agatcggtgc ctgcacaacc atcgatcgcg gcgcgctgga tgacactgtt
    43681 attggcaatg gcgtgatcat tgataaccag tgccagattg cacataacgt cgtgattggc
    43741 gacaatacgg cggttgccgg tggcgtcatt atggcgggca gcctgaaaat tggtcgttac
    43801 tgcatgatcg gcggagccag cgtaatcaac gggcatatgg aaatatgcga caaagtgacg
    43861 gttacgggca tgggtatggt gatgcgtccc atcactgaac caggcgtcta ttcctcaggc
    43921 attccgctgc aacccaacaa agtctggcgc aaaaccgctg cactggtgat gaacattgat
    43981 gacatgagca agcgtctgaa atcgcttgag cgcaaggtta atcaacaaga ctaacgttcc
    44041 atcttttgtt cgccaaactt tacggcctgt ctcattctta cgattgcggc aggccgtgtt
    44101 attattgtcg tttcttatat tttgacagga agagtatctt gactactaac actcatactc
    44161 tgcagattga agagatttta gaacttctgc cgcaccgttt cccgttctta ctggtggatc
    44221 gcgtgctgga ttttgaagaa ggtcgttttc tgcgcgcagt aaaaaatgtc tctgtcaatg
    44281 agccattctt ccagggccat ttccctggaa aaccgatttt cccgggtgtg ctgattctgg
    44341 aagcaatggc acaggcaaca ggtattctgg cgtttaaaag cgtaggaaaa ctggaaccgg
    44401 gtgagctgta ctacttcgct ggtattgacg aagcgcgctt caagcgcccg gtcgtgcctg
    44461 gcgatcaaat gatcatggaa gtcactttcg aaaaaacgcg ccgcggcctg acccgtttta
    44521 aaggggttgc tctggtcgat ggtaaagtag tttgcgaagc aacgatgatg tgtgctcgta
    44581 gccgggaggc ctgatacgtg attgataaat ccgcctttgt gcatccaacc gccattgtgg
    44641 aagagggcgc gtcaattggc gcgaacgcac acattggtcc tttttgtatc gttggacccc
    44701 atgtcgaaat tggtgagggt accgtactga aatctcacgt tgtcgtgaat ggtcatacta
    44761 aaattggccg cgataatgag atttatcagt ttgcctccat cggcgaagtt aaccaggatt
    44821 tgaaatatgc tggcgaaccg acccgtgtgg aaatcggcga tcgtaaccgc attcgcgaaa
    44881 gcgtcaccat tcatcgtggc acagtccagg gcggtggatt gacgaaggtg ggcagcgaca
    44941 acttactgat gatcaacgcg cacattgcgc acgattgtac ggtaggtaac cgctgtattc
    45001 tcgccaacaa cgcaacgctg gcgggtcacg tatcggttga cgacttcgcg atcatcggcg
    45061 gcatgaccgc agtccatcag ttctgcatca ttggtgcgca tgtgatggtt ggcggctgct
    45121 ccggtgtggc gcaggacgtc cctccttatg tcattgcgca gggtaaccac gcaacgccgt
    45181 tcggtgtcaa tatcgaaggg ctgaagcgcc gcggattcag ccgtgaggcg attaccgcta
    45241 tccgcaatgc gtataagcta atttatcgta gcggtaaaac gctcgatgaa gtgaaaccgg
    45301 aaattgctga actggcggaa acatatccgg aagtgaaagc ctttaccgat ttctttgcac
    45361 gctcaacgcg cggtctgatt cgttaatgac tgaccagcgt ccattaacga ttgccctggt
    45421 cgccggagaa acctccggcg atatcctggg ggccggttta atccgcgctc tgaaagaacg
    45481 tgtgcccaac gcccgctttg ttggtgttgc cgggccacga atgcaggctg aaggctgcga
    45541 agcctggtac gaaatggaag aactggcggt gatgggcatt gttgaagtgc tcggtcgtct
    45601 gcgtcgctta ctgcatattc gtgccgatct gacaaagcgt tttggcgaac tgaagccaga
    45661 tgtttttgtt ggtattgatg cgcccgactt caatattact cttgaaggta acctcaaaaa
    45721 gcagggtatc aaaaccattc attacgtcag tccatccgtc tgggcgtggc gacagaaacg
    45781 cgttttcaaa ataggcagag ccaccgatct ggtgctcgca tttctgcctt tcgaaaaagc
    45841 gttttatgac aaatacaatg taccgtgccg ctttatcggt cataccatgg ctgatgccat
    45901 gccgttagat ccagataaaa atggtgcccg tgatgtgctg gggatccctt acgatgccca
    45961 ctgtctggca ttgttgccgg gcagccgtgg tgcagaagtc gaaatgctta gtgccgattt
    46021 cttgaaaact gcccagcttt tgcgccagac atatccggat ctcgaaatcg tggtgccgct
    46081 ggtgaatgcc aaacgccgcg agcagtttga acgcatcaaa gctgaagtcg cgccagacct
    46141 gtcggttcat ttgctggatg gaatgggccg tgaggcgatg gtcgccagcg atgcggcact
    46201 actggcgtcg gggacggcag ccctggagtg tatgctggcg aaatgcccga tggtggtggg
    46261 atatcgcatg aagcctttta ccttctggtt ggcgaagcgg ctggtgaaaa ctgattatgt
    46321 ctcgctgcca aatctgctgg cgggcagaga gttagtcaaa gagttattgc aggaagagtg
    46381 tgagccgcaa aaactggctg cggcgctgtt accgctgttg gcgaacggga aaaccagcca
    46441 cgcgatgcac gataccttcc gtgaactgca tcagcagatc cgctgcaatg ccgatgagca
    46501 ggcggcacaa gccgttctgg agttagcaca atgatcgaat ttgtttatcc gcacacgcag
    46561 ctggttgcgg gtgtggatga agtcggacgc gggccgttag ttggcgcggt cgtcaccgct
    46621 gcggtgatcc ttgacccggc gcgcccgatt gccgggctga atgattccaa aaagctgagc
    46681 gaaaaacgcc gtctggcgct ctatgaagag atcaaagaga aagcgttgag ctggagtctg
    46741 ggccgcgcgg aaccccacga aatcgacgag ctgaacattc ttcatgcgac catgctggcg
    46801 atgcagcgtg ccgtcgctgg gctgcatatt gcgccggaat atgtgttgat tgatggtaac
    46861 cgctgcccga aattaccgat gcctgcgatg gctgtggtga aaggggatag ccgcgtacca
    46921 gaaatcagtg ccgcgtctat cctggcgaaa gtgacgcgtg acgccgaaat ggcggcgctg
    46981 gatattgttt tcccgcaata tggttttgcc caacataaag ggtatccaac cgcttttcat
    47041 ctggaaaaac tggctgaata tggcgcgact gaacatcatc gtcgcagctt tgggcctgtc
    47101 aaacgcgcac tgggacttgc gtcctgattc ttgtgtcgag attaagtaaa ccggaatctg
    47161 aagatgtctg aaccacgttt cgtacacctg cgggtgcaca gcgactactc gatgatcgat
    47221 ggcttggcca aaaccgcacc gctggtaaaa aaggcggcgg cgttgggtat gccagcactg
    47281 gcgatcaccg atttcaccaa cctttgcggt ctggtgaagt tctacggagc gggacatggc
    47341 gcagggatta agcctatcgt cggggcagat tttaatgtcc agtgcgacct gctgggtgat
    47401 gagttaaccc acctgacggt actggcggcg aacaataccg gctatcagaa tctggcgttg
    47461 ctgatctcaa aagcgtatca gcgcgggtac ggtgccgccg ggccgatcat cgatcgcgac
    47521 tggcttatcg aattaaacga agggttgatc cttctttccg gcgggcgcat gggcgacgtc
    47581 ggacgcagtc ttttgcgtgg taacagcgcg ctggtagatg agtgtgtcgc gttttatgaa
    47641 gaacacttcc cggatcgcta ttttctcgag ctgatccgca ccggcaggcc ggatgaagaa
    47701 agctatctgc acgcggcggt ggaactggcg gaagcgcgcg gtttgcccgt cgtggcgact
    47761 aacgatgtgc gctttatcga cagcagcgac tttgacgcac acgaaatccg cgtcgcgatc
    47821 cacgacggct ttaccctcga cgatcctaaa cgcccgcgta actattcgcc gcagcaatat
    47881 atgcgtagcg aagaggagat gtgcgagctg tttgccgaca tcccagaagc ccttgccaac
    47941 accgttgaga tcgccaaacg ctgtaacgta actgtgcgtc ttggtgaata cttcctgccg
    48001 cagttcccga ccggggacat gagcaccgaa gattatctgg tcaagcgtgc aaaagaaggc
    48061 ctggaagagc gtctgacatt tttatttcct gacgaggaag aacgtcttaa acgccgcccg
    48121 gaatatgacg aacgtctgga gactgaactt caggttatca accagatggg cttcccgggc
    48181 tacttcctca tcgttatgga atttatccag tggtcgaaag ataacggcgt accggtaggg
    48241 ccaggccgtg gctccggtgc gggttcactg gtagcctacg cgctgaaaat caccgacctc
    48301 gatccgctgg aatttgacct gctgttcgaa cgtttcctta acccggaacg tgtctccatg
    48361 cctgacttcg acgttgactt ctgtatggag aaacgcgatc aggttatcga acacgtggcg
    48421 gacatgtacg gtcgtgatgc ggtatcgcag attatcacct tcggtacgat ggcggcgaaa
    48481 gcggtgatcc gcgacgtagg ccgcgtgctg gggcatccgt acggctttgt cgaccgtatc
    48541 tcgaaactga tcccgcccga tccggggatg acgctggcga aagcgtttga agccgagccg
    48601 cagctgccgg aaatctacga agcggatgaa gaagttaagg cgctgatcga catggcgcgc
    48661 aaactggaag gggtcacccg taacgccggt aagcacgccg gtggggtggt tatcgcgccg
    48721 accaaaatta ccgattttgc gccgctttat tgcgatgaag agggcaaaca tccggtcacc
    48781 cagtttgata aaagcgacgt tgaatacgcc ggactggtga agttcgactt ccttggtttg
    48841 cgtacgctca ccatcattaa ctgggcgctg gagatgatca acaagcggcg ggcgaagaat
    48901 ggcgagccac cgctggatat cgccgcgatc ccgctggatg acaagaaaag cttcgacatg
    48961 ctgcaacgct cggaaaccac ggcggtattc cagcttgaat cgcgcggcat gaaggacctg
    49021 atcaagcgtc tgcaacctga ctgcttcgaa gatatgatcg cactggtggc actgttccgc
    49081 cctggtccgt tgcagtcagg gatggtggat aactttatcg accgtaaaca tggtcgtgaa
    49141 gagatctcct atccggacgt gcagtggcag catgaaagcc tgaaatcggt actggagcca
    49201 acctacggca ttatcttgta tcaggaacag gtcatgcaga ttgcccaggt gctttctggt
    49261 tataccctcg gtggcgcgga tatgctgcgc cgtgcgatgg gtaagaaaaa gccggaagag
    49321 atggctaagc agcgttctgt atttgctgaa ggtgcagaaa agaacggaat caacgccgaa
    49381 ctggcgatga aaatcttcga cctggtggag aaattcgcgg gttacggatt taacaaatcg
    49441 cactctgcgg cctatgcttt ggtgtcatat caaacgttat ggctgaaagc gc
//
LOCUS       DADLSA010000012        49326 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.012, whole genome shotgun sequence.
ACCESSION   DADLSA010000012 DADLSA010000000
VERSION     DADLSA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.012"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(82..1212)
                     /gene="dnaJ"
                     /locus_tag="JN691_000728"
     CDS             complement(82..1212)
                     /gene="dnaJ"
                     /locus_tag="JN691_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAY7242243.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1301..3217)
                     /gene="dnaK"
                     /locus_tag="JN691_000729"
     CDS             complement(1301..3217)
                     /gene="dnaK"
                     /locus_tag="JN691_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAY7242244.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3594..3998
                     /locus_tag="JN691_000730"
     CDS             3594..3998
                     /locus_tag="JN691_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAY7242245.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4024..4737
                     /gene="msyB"
                     /locus_tag="JN691_000731"
     CDS             4024..4737
                     /gene="msyB"
                     /locus_tag="JN691_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAY7242246.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLHGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            4886..5452
                     /gene="satP"
                     /locus_tag="JN691_000732"
     CDS             4886..5452
                     /gene="satP"
                     /locus_tag="JN691_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAY7242247.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNITGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5487..6074)
                     /gene="mog"
                     /locus_tag="JN691_000733"
     CDS             complement(5487..6074)
                     /gene="mog"
                     /locus_tag="JN691_000733"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAY7242248.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLASALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6189..7142)
                     /gene="tal"
                     /locus_tag="JN691_000734"
     CDS             complement(6189..7142)
                     /gene="tal"
                     /locus_tag="JN691_000734"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY7242249.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7421..8851
                     /locus_tag="JN691_000735"
     CDS             7421..8851
                     /locus_tag="JN691_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HAY7242250.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGFWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            8921..9697
                     /gene="yaaA"
                     /locus_tag="JN691_000736"
     CDS             8921..9697
                     /gene="yaaA"
                     /locus_tag="JN691_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAY7242251.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(9850..10146)
                     /locus_tag="JN691_000737"
     CDS             complement(9850..10146)
                     /locus_tag="JN691_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY7242252.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10360..11646)
                     /gene="thrC"
                     /locus_tag="JN691_000738"
     CDS             complement(10360..11646)
                     /gene="thrC"
                     /locus_tag="JN691_000738"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAY7242253.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(11647..12579)
                     /gene="thrB"
                     /locus_tag="JN691_000739"
     CDS             complement(11647..12579)
                     /gene="thrB"
                     /locus_tag="JN691_000739"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAY7242254.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12581..15043)
                     /gene="thrA"
                     /locus_tag="JN691_000740"
     CDS             complement(12581..15043)
                     /gene="thrA"
                     /locus_tag="JN691_000740"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAY7242255.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVFSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLDGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(15124..15189)
                     /gene="thrL"
                     /locus_tag="JN691_000741"
     CDS             complement(15124..15189)
                     /gene="thrL"
                     /locus_tag="JN691_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAY7242256.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15403..16089)
                     /locus_tag="JN691_000742"
     CDS             complement(15403..16089)
                     /locus_tag="JN691_000742"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY7242257.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16489..16629)
                     /locus_tag="JN691_000743"
     CDS             complement(16489..16629)
                     /locus_tag="JN691_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242258.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            16725..17441
                     /gene="arcA"
                     /locus_tag="JN691_000744"
     CDS             16725..17441
                     /gene="arcA"
                     /locus_tag="JN691_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAY7242259.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17500..18852)
                     /gene="creD"
                     /locus_tag="JN691_000745"
     CDS             complement(17500..18852)
                     /gene="creD"
                     /locus_tag="JN691_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAY7242260.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(18910..20334)
                     /gene="creC"
                     /locus_tag="JN691_000746"
     CDS             complement(18910..20334)
                     /gene="creC"
                     /locus_tag="JN691_000746"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAY7242261.1"
                     /translation="MRIGMRLLRGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSVNKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPSEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20334..21023)
                     /gene="creB"
                     /locus_tag="JN691_000747"
     CDS             complement(20334..21023)
                     /gene="creB"
                     /locus_tag="JN691_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAY7242262.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21036..21509)
                     /gene="creA"
                     /locus_tag="JN691_000748"
     CDS             complement(21036..21509)
                     /gene="creA"
                     /locus_tag="JN691_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAY7242263.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            21720..22589
                     /gene="robA"
                     /locus_tag="JN691_000749"
     CDS             21720..22589
                     /gene="robA"
                     /locus_tag="JN691_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAY7242264.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22586..23233)
                     /gene="gpmB"
                     /locus_tag="JN691_000750"
     CDS             complement(22586..23233)
                     /gene="gpmB"
                     /locus_tag="JN691_000750"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAY7242265.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23285..23806
                     /gene="yjjX"
                     /locus_tag="JN691_000751"
     CDS             23285..23806
                     /gene="yjjX"
                     /locus_tag="JN691_000751"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HAY7242266.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(23891..24217)
                     /gene="trpR"
                     /locus_tag="JN691_000752"
     CDS             complement(23891..24217)
                     /gene="trpR"
                     /locus_tag="JN691_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAY7242267.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24307..26244)
                     /gene="sltY"
                     /locus_tag="JN691_000753"
     CDS             complement(24307..26244)
                     /gene="sltY"
                     /locus_tag="JN691_000753"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAY7242268.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGHSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26455..28122
                     /gene="ettA"
                     /locus_tag="JN691_000754"
     CDS             26455..28122
                     /gene="ettA"
                     /locus_tag="JN691_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAY7242269.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28430..29662)
                     /gene="nadR"
                     /locus_tag="JN691_000755"
     CDS             complement(28430..29662)
                     /gene="nadR"
                     /locus_tag="JN691_000755"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HAY7242270.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(29683..31065)
                     /gene="radA"
                     /locus_tag="JN691_000756"
     CDS             complement(29683..31065)
                     /gene="radA"
                     /locus_tag="JN691_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAY7242271.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31114..32082)
                     /gene="serB"
                     /locus_tag="JN691_000757"
     CDS             complement(31114..32082)
                     /gene="serB"
                     /locus_tag="JN691_000757"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAY7242272.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LQMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32188..32832
                     /locus_tag="JN691_000758"
     CDS             32188..32832
                     /locus_tag="JN691_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAY7242273.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            32860..33876
                     /gene="lplA"
                     /locus_tag="JN691_000759"
                     /pseudo
     CDS             32860..33876
                     /gene="lplA"
                     /locus_tag="JN691_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            33908..34171
                     /locus_tag="JN691_000760"
     CDS             33908..34171
                     /locus_tag="JN691_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7242274.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(34332..35051)
                     /gene="deoD"
                     /locus_tag="JN691_000761"
     CDS             complement(34332..35051)
                     /gene="deoD"
                     /locus_tag="JN691_000761"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAY7242275.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35108..36331)
                     /gene="deoB"
                     /locus_tag="JN691_000762"
     CDS             complement(35108..36331)
                     /gene="deoB"
                     /locus_tag="JN691_000762"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY7242276.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(36383..37705)
                     /gene="deoA"
                     /locus_tag="JN691_000763"
     CDS             complement(36383..37705)
                     /gene="deoA"
                     /locus_tag="JN691_000763"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAY7242277.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(37832..38611)
                     /gene="deoC"
                     /locus_tag="JN691_000764"
     CDS             complement(37832..38611)
                     /gene="deoC"
                     /locus_tag="JN691_000764"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAY7242278.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            38869..40410
                     /locus_tag="JN691_000765"
     CDS             38869..40410
                     /locus_tag="JN691_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAY7242279.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDEIIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            40382..41245
                     /gene="yjjW"
                     /locus_tag="JN691_000766"
     CDS             40382..41245
                     /gene="yjjW"
                     /locus_tag="JN691_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAY7242280.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNGCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(41459..42241)
                     /locus_tag="JN691_000767"
     CDS             complement(41459..42241)
                     /locus_tag="JN691_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7242281.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(42238..43311)
                     /locus_tag="JN691_000768"
     CDS             complement(42238..43311)
                     /locus_tag="JN691_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAY7242282.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(43433..43594)
                     /locus_tag="JN691_000769"
     CDS             complement(43433..43594)
                     /locus_tag="JN691_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAY7242283.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(43721..44326)
                     /gene="osmY"
                     /locus_tag="JN691_000770"
     CDS             complement(43721..44326)
                     /gene="osmY"
                     /locus_tag="JN691_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HAY7242284.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(44719..46308)
                     /gene="prfC"
                     /locus_tag="JN691_000771"
     CDS             complement(44719..46308)
                     /gene="prfC"
                     /locus_tag="JN691_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAY7242285.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(46399..47076)
                     /gene="yjjG"
                     /locus_tag="JN691_000772"
     CDS             complement(46399..47076)
                     /gene="yjjG"
                     /locus_tag="JN691_000772"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAY7242286.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(47091..47537)
                     /gene="rimI"
                     /locus_tag="JN691_000773"
     CDS             complement(47091..47537)
                     /gene="rimI"
                     /locus_tag="JN691_000773"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAY7242287.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(47506..47919)
                     /gene="holD"
                     /locus_tag="JN691_000774"
     CDS             complement(47506..47919)
                     /gene="holD"
                     /locus_tag="JN691_000774"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAY7242288.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            48022..49053
                     /gene="rsmC"
                     /locus_tag="JN691_000775"
     CDS             48022..49053
                     /gene="rsmC"
                     /locus_tag="JN691_000775"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAY7242289.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        11552 a        13449 c        12445 g        11880 t
ORIGIN      
        1 ccgccacttt acaggtgctc gcatatcttc aacgcaccct atttttaccc aggcctgccc
       61 acgggcaggc ttttggggag gttagcgggt caggtcgtca aaaaacttct tcacaccatc
      121 aaagaagctc tttgagcgcg ggctgttgtg ctcgccggtt gggccaccga agctttcttg
      181 cagctcttgc agcagctgtt tctgcttttc gttcaggcct accggtgttt cgacgacaac
      241 gcggcacagc aaatcaccct gtgcgccacc gcggacagac ttgacgcctt taccgcgcat
      301 acggaatagc ttaccggtct gggtttcgcc aggcactttc agtttgacgc gaccatcaag
      361 ggtcggtact tcgatttcgc cacccagcgc cgccatagcg aagttgatcg ggacttcgca
      421 atacaggttg ttgccttcac gctcgaaaat cgggtgctgt ttaacctgaa cctgaacgta
      481 cagatcgcct gccggtgcgc catgctcgcc cgcttcacct tcgcccgcaa gacggatgcg
      541 gtctccagtg tccacccctg ccgggatttt aacggacagc gttttgctgc gctcaacacg
      601 accatgacca tgacatttgt tgcacggatc tttgatcagc gtaccgcggc cctgacagtg
      661 tggacaggtc tgctgtacag cgaagaatcc ctggcgcatc tgcacctgac cagaaccatg
      721 acaggtcgga caggtctgcg gctgtgtacc tggttttgca ccgctaccgt ggcaaacgtc
      781 acactcttcc agagtcggaa tgcggatctc tttggtcacg ccacgtacag cttcttcgag
      841 ggtgagctcc atgttatagc gtaaatcagc accgcgcgcc gcacgttgac gaccacgtcc
      901 gccgccaaaa atatcgccga aaacgtcacc aaaaatatcg ctgaagtctg cgccgccgcc
      961 aaaaccaccg ccgcccatgc caccttgctc aaacgcagca tgaccatact gatcgtatgc
     1021 cgcacgtttt tgcgagtcgg tcagaacttc ataagcttcc ttgatctctt taaatttcgc
     1081 ctcggcctct ttgtcaccct ggttacggtc cgggtggtat ttcatggcca gccgtttgta
     1141 ggcctttctg atttcacgct cttccgctgt tttggaaacg cctaaaatct cgtaataatc
     1201 ttgcttagcc atctttttta aattgcccct agatgaatgc acgggcggag aggaaattcc
     1261 ccttcgcccg tgtcagtata attacccgtt tatagggcga ttattttttg tctttgactt
     1321 cttcaaattc agcgtcgaca acatcgtcat ctttcgcgtt gtttgcagaa gcatcagcac
     1381 cggcagtctg ctgctgggca tgttgctgct gggcgatttc catcagtttc tgggaaacct
     1441 gtgccagttc ctgcattttc gcttcgatag cggctttgtc ttcacctttc agagcagttt
     1501 ccagtgcagt cagcgcagac tcgatagcag ttttgtcgtc agccggcagt ttgtcgcctg
     1561 cttcttcaac ctgcttacgg gtgctgtgca gcagatggtc gccctggttg cgagtctgta
     1621 ccagctcttc aaacttacgg tcagcttcgg cgttagcttc tgcgtcgcgt accattttct
     1681 ggatttcatc ttcgttcaga ccagaagaag ccttgatggt gatcttctgc tctttaccgc
     1741 tgtttttatc tttcgcggaa acgtgcagga taccgtcagc atcgatatcg aaggtaactt
     1801 cgatctgcgg catgccgcgc ggtgccgggt tgataccatc taggttgaac tgacccagag
     1861 atttgttatc agccgcacgt ttacgttcac cctgcagcac atggatggtt accgcagact
     1921 ggttgtcttc agcggtagag aacacctggc tgtgcttggt cgggatagtg gtgtttttcg
     1981 cgatcagcgt cgtcatcaca ccgcccatgg tttcgatacc cagagacagc ggggtaacgt
     2041 ccagcagcag tacgtctttt acgtcaccag tcagaacacc accctgaaca gcagcaccga
     2101 ttgctacagc ttcgtccggg ttaacgtctt tacgcggctc tttaccaaag aactcagcaa
     2161 ctttcttctg aaccattggc atacgagtct gaccaccaac gaggataacg tcgtcgatat
     2221 cagatacgga caggccagca tcctgcagtg caactttcag cggctcaatg gaacggttta
     2281 ccagatcttc aaccaggctt tccagtttcg cacgagtcac tttgatgttc atgtgtttcg
     2341 gaccggtcgc gtctgcagtg atgtatggca ggttaacgtc ggtctgctga gcggaagaca
     2401 gttcgatttt cgctttttct gccgcttctt tcaggcgctg cattgccagc ggatcgttgc
     2461 gcaggtcaat gccctgatct ttcttgaatt cttcaaccag atagttgatc agacggctgt
     2521 cgaagtcttc accacccagg tgggtatcac cgttggttgc cagaacttcg aaggtttttt
     2581 cgccgtcaac ttcgtcgatt tcgataatag aaatatcgaa agtaccaccc cccaggtcat
     2641 aaaccgcgat agtacggttg ccagtgcctt tgtccagacc gtaagccagc gcagctgcgg
     2701 tcggttcgtt gatgatacgt tttacttcca gaccagcgat acggcctgcg tctttggttg
     2761 cctgacgctg agcatcgtta aagtatgccg gtacggtgat aacagcttca gttaccggtt
     2821 cacccaggta atcttcagcg gttttcttca tttttttcag cacttcagca gaaatctgcg
     2881 gcggtgccat tttctggcct ttaacttcga cccatgcgtc gccgttatca gcagcaataa
     2941 ttttgaacgg catgatggaa acatcacgct gtacttcttc gtcctggaag cggcggccaa
     3001 tcaggcgttt aatcgcaaac agggtgtttt gcgggttcgt cactgcctga cgtttagccg
     3061 gctgaccaac cagagtttca ccatcctggg tataggcaat gatagaaggc gtggtgcgat
     3121 cgccttcggc gttctccagc acgcgaggag tggtgccatc cataatcgct acacaagagt
     3181 tggtagtacc caggtcgata ccaattattt tacccatcta aacgtctcca ctatatattc
     3241 ggtcatcatg tggttgtgag tctgtaatag gggcgaaacg tctggtttca actgcccaat
     3301 ttcatttttt tgcagactca ctcactgcgg ttgactacta aatggggtcg taacccacgt
     3361 catcaagggg gagatgcaaa aattttttgt gcttttgcgc agaaatttcg cgatatggca
     3421 ggacaagacg taaaattgac agcaatttta ttcatataaa gaatgaatcg cagccaaggc
     3481 attcatcaaa aaattgcaat aaaaagaaaa gattacgtgc ctgaatcttc tctttatcag
     3541 cagtaaacta gtgggtattc atccccctac ctcttcccac taagagaatc cttatgaaat
     3601 ccgtttttac gatttccgcc agcctggcga ttagcctgat gctgtgctgc acggcgcagg
     3661 caaacgacca taaactcctc ggcgtcattg caatgccgcg taacgaaacc aacgatctgg
     3721 cgctgaaact tcctgtttgt cgcattgtga aacgcataca actctctgcc gaccatggcg
     3781 atttacagtt aagcggtgca tcggtttatt tcaaagccgc ccgtagcgcc agtcagagcc
     3841 tgaatattcc ttcagaaata aaagaagggc aaaccactga ctggatcaac attaacagcg
     3901 ataacgacaa taaacgctgc gtctcaaaaa tcaccttttc cggtcatacg gtgaactcat
     3961 cggatatggc cacgctgaaa attatcggcg acgactaacg ccagaatata gaagccacaa
     4021 aaaatgaatg ttaattacct gaatgattca gatctggatt ttcttcagca ttgtagtgag
     4081 gaacagttgg caaatttcgc ccgattgctc acccataatg aaaaaggcaa aactcgcctc
     4141 tccagcgtac tgatgcgcaa cgaactgttt aaatcgatgg aagggcatcc cgagcaacat
     4201 cgccgcaact ggcagctgat tgccggagaa ttacagcatt ttggtggcga tagtatcgcc
     4261 aacaaactgc gcggacacgg taaattgtat cgggccattt tgctcgatgt ctcaaagcga
     4321 ttgaagctga aagccgacaa agagatgtct acgtttgaaa ttgagcaaca gttactggaa
     4381 caatttctgc gtaatacctg gaagaaaatg gacgaggaac ataagcagga gtttctgcac
     4441 gcggtcgatg ccagggtgaa tgagctggaa gagctgctgc cgctgctgat gaaagacaaa
     4501 ttattggcaa aaggtgtgtc gcatttgctt tccagccaac tgacccgcat tttacgcacc
     4561 cacgcagcaa tgagcgtact tgggcatggt ttgctgcacg gcgcggggct gggaggccct
     4621 gtgggcgcag cattaaatgg ggttaaagcg gtcagcggca gcgcctatcg cgtgacgatt
     4681 ccagccgtac tgcaaatcgc ctgcctgcgc cggatggtta gcgccactca ggtctgatac
     4741 cggaaactgg ggaaaatttt cattttcccc tcaaaagatc gtagacactg ccccactggc
     4801 tgattattat gccgcgccct gaaaacacta cagttattca gggaaattat ttcaccattc
     4861 attcgatgat gatttttgag gaattatggg caacactaag ttggctaatc cggcaccgct
     4921 gggcctgatg ggcttcggca tgaccaccat tctgcttaac ctgcacaacg tgggttattt
     4981 cgctctggac ggtattattc ttgccatggg cattttctac ggcggcatcg cgcaaatttt
     5041 tgctggtctg ctggagtaca aaaaaggcaa cactttcggt ttaaccgcat tcacctctta
     5101 cggttctttc tggctgacgc tggttgcgat tctgctgatg ccgaaactgg gtctgaccga
     5161 tgcgccaaat gcacagttcc ttggtgtcta cctgggtctg tggggcgtat ttacgctgtt
     5221 tatgttcttc ggcactctga aaggcgcacg cgtcctgcaa ttcgttttct ttagcctgac
     5281 cgtgctgttt gccctgctgg cgatcggtaa cattaccggt aatgccgcga tcatccactt
     5341 tgccggctgg attggtctga tctgcggtgc cagcgcaatc tatctggcga tgggtgaagt
     5401 gctgaacgag cagtttggtc gcaccgttct gccgattggt gaatcccact aattgtcttg
     5461 agatcccccc gctcgggggg atttttttat tcgctaacgt cgcgtcttgc actcttcggt
     5521 ctgaatgcag caaccacttc tggtgccgtt tcaacgtatg gcccttccag caactgaatg
     5581 cagtacggta cgctggcaaa aataccatgc accacaacgt taccctcagc gtccttcaca
     5641 ccttccagcg tctctttaat agacttcggc tgaccgggta agttaaggat cagcgcctgt
     5701 ttgcgaatca cgcccacctg acgcgaaagg atcgcagttg gtacaaaatg caggctgatc
     5761 tggcgcatct gttcaccaaa gccaggcatc tcgcggtccg ctactgccag cgtcgcatcg
     5821 ggcgttacgt cacgacgcgc cgggccagtt ccgcccgtgg tgagcaccag atggcaactc
     5881 atttcatcca ccagctcaca caacgtttgc tcgatgatcg cctgctcatc ggggattaag
     5941 cgggtttcca gttcaaacgg cgtggttagc gccgatgcca gccattcttc cagcgctgga
     6001 atgcctttat cctgataaac gccgctggat gcgcgatcag agatggaaac taagccaata
     6061 cgtaaagtat tcatcaagat ttttccgttt aaacagtctc gttaaacaga atgatacact
     6121 gcgaagggag tgacagacag gcttcagaag aggtagcgtg accgacttcc cggtcacgct
     6181 aagaatgatt acagcagatc gccgatcatt ttttccagtt tttcctggtc aatagcaaac
     6241 ttacggatac cttccgccag tttatctact gccattggat cctggttgtg ctgccacagg
     6301 aactcggact cagtgatacg cgccggacgc gctttcactt cgccggtgta agacagttta
     6361 cgttcgatag ccccttcgct ctccgccagc tctttcagca gtgccggtgc gatggtcaga
     6421 cggtcacagc ctgccagttc cagaatttcg ccgatgttac ggaagcttgc gcccataacc
     6481 acggtttcat aaccgtgctc tttgtagtac tggtagattt cagatacaga aaccacgccc
     6541 ggatcttctg ccggagcgta ctctttctta tcggtattcg ctttgtacca gtcaagaata
     6601 cggccaacaa acggcgagat caggaacacg cccgcctccg cacaagcacg agcctgagcg
     6661 aaggagaaca gcagggtcag gttacagttg ataccttctt tttccagctg ttctgcagca
     6721 cggataccct gccaggtaga agccagtttg atcagaatac gatcgttgct aatacctgca
     6781 tcgttgtaga gtttgatcag gcgttttgct ttcgcaattg acgcttcggt gtcataggaa
     6841 agacgcgcat caacttcagt tgagatacgg cccggaacca gtttcaggat ttccagacca
     6901 atatttactg ccagtttgtc ggtcgcgtcc acgatctgct gcgcgcgatc gttgctctgc
     6961 tgtttcgccc aggcgacagc atcatcaatc aacttacggt attccggaat ctgcgctgcg
     7021 ttaagaatga gagaagggtt ggttgtggca tcctgcggtt gatacagctt cattgccgcg
     7081 atgtccccag tgtcggccac tacggtggtg tactgacgaa gggaggtcaa tttgtccgtc
     7141 atgatagtat ttctctttaa acagcttgtt agggggatgt aaccggtctg ccctgatgat
     7201 atcacgacgc aacgtcagcg caaccgccga caagacggtt atcgccaggc gagactgttt
     7261 cggatttctg acactgcgtt gatatcgccc gccattttta tacaaaacct catgtatgct
     7321 acgcagaagt tatcaagtac ctcgtagcgt atatacttct taaacaattg gtaacgttta
     7381 cacaggaaag tcatcgcgac cggcaataag agggatatgc atgccagatt ttttctcctt
     7441 tattaacagc gtcctttggg gatcggtaat gatttacctg ctcttcggcg caggttgttg
     7501 gttcactttt cgcaccggat ttgtgcagtt tcgctacatc cgccagtttg gcaaaagtct
     7561 taaaaatagc attcatccac agccaggcgg tttaacctca tttcagtcat tgtgtaccag
     7621 tcttgcggcg cgcgtgggta gcggcaacct ggccggcgtt gcgctggcaa ttaccgccgg
     7681 tggaccaggt gccgtctttt ggatgtgggt tgctgcgttt atcggcatgg cgacctcgtt
     7741 tgccgaatgt tcccttgcac aactttataa agaacgtgac gtcaatgggc agtttcgtgg
     7801 cggaccggca tggtatatgg cgcgcgggct ggggatgcgc tggatgggcg ttctgttcgc
     7861 cgtctttttg ctcatcgcct acggcataat tttcagcgga gttcaggcga acgccgttgc
     7921 ccgagccctg agtttttctt ttgattttcc tccgctggtg acaggcatta ttctcgctgt
     7981 ctttgctctg ctggcaatca ctcgcggtct tcatggcgtc gcccggctca tgcaggggtt
     8041 tgtcccgttg atggcgataa tctgggtact gaccagcctg gtgatttgcg taataaatat
     8101 cgggcaactt ccccacgtca tttggtctat ttttgagagt gcttttggct ggcaggaagc
     8161 ggcaggcggc gcggcgggat ataccttaag ccaggcgatt actaacggtt ttcagcgcag
     8221 tatgttttcc aatgaggcgg gaatgggatc gacgccaaac gcggcagcgg cagctgcgtc
     8281 ctggcctccg catccggcag cacaagggat tgtccagatg attggcattt ttatcgacac
     8341 cctggttatc tgtacggcaa gcgccatgct gatattactg gcgggtaacg gcacaaccta
     8401 catgccgctg gaaggtattc agcttatcca gaaggcgatg cgggtgctaa tgggtttctg
     8461 gggtgctgaa tttgttaccc tcgtggttat tctgtttgcc ttcagctcca tcgttgccaa
     8521 ctacatttat gccgaaaaca atctcttctt tttacgcctg aacaacccta aagcgatctg
     8581 gtgtttgcgg atctgcacct tcgcaacggt catcggcggc accttgctaa gccttccgct
     8641 gatgtggcaa ctggcagata tcataatggc ctgcatggct attaccaatt tgaccgccat
     8701 tttactgctc tcgcctgtgg ttcataccat tgccagtgat tatctacgcc agcgtaaact
     8761 cggcgtgcgc ccggtgtttg atccgttgcg ttatccggat atcggtcgcc agctttctcc
     8821 ggacgcgtgg gatgatgttt cgcaggagta atcacaacta tcgatcaact cattctcttt
     8881 ttttgctaaa gtcggcataa atttcctgca aggactggat atgctgattc ttatttcacc
     8941 tgcgaaaacg cttgattacc aaagcccgtt gaccaccacg cgctatacgc tgccggagct
     9001 gttagacaat tcccagcagt tgatccatga agcgcggaaa ctgacgcctc cgcagattag
     9061 cacgctgatg cgcatcagcg acaaactggc gggtatcaac gccgctcgct ttcatgactg
     9121 gcagccagat ttcacgccgg cgaatgcccg ccaggcgatt ctggcgttta aaggtgatgt
     9181 ctacaccggc ttgcaggccg aaaccttcag cgaagacgat ttcgattttg cccaacagca
     9241 tttgcgaatg ctttccggct tgtatggcgt actgcgcccg ctcgatttaa tgcagcctta
     9301 tcgtctggaa atggggatcc gtcttgagaa tgcccgaggg aaagatctgt atcaattctg
     9361 gggagatatc atcaccaaca agctgaacga ggcgctcgca gcacaaggcg ataatgtggt
     9421 gattaatctg gcgtcagatg agtattttaa atcggtgaag ccgaagaaat tgaatgccga
     9481 gattatcaag ccggtgttcc tcgatgagaa gaacggcaaa tttaagatca tcagcttcta
     9541 cgctaagaaa gcacgcggtc tgatgagtcg tttcattatt gaaaaccggc tgaccaaacc
     9601 agagcaactg actggtttta atagcgaagg ttacttcttt gatgaagatt cctccagcaa
     9661 tggcgaactg gtgtttaaac gctacgagca gcgttaagtt tgtagtcaat gccggatgcg
     9721 gcgtgaacgc cttatccggc ctacaaaagc atgcagattc aatatattgc agaattaacg
     9781 taggcctgat aagacgcgcc agcgtcgcat caggcgctga atgccggatt gttacccggc
     9841 atttgtcatt tagcggtgat gtttgcctgg accatgaccg ccgtgatgat catgaggagc
     9901 tttcttatgg tggcgcggcg gtggcggcgg tccgtgtggg tgccagcgat tgcctcgcca
     9961 ttcataatgt tgtttccacc agccgtggtc gcgccagtga cctccatccc agtaatagcc
    10021 acgattatca cgatcgccta tctgtaattt tactgacggg actaacgtaa tttccgcagc
    10081 ctgtgctgcc atgggagcga ccagaaccag ggaaagtgcg agtacgatag attgcatctt
    10141 tttcacaggt tactcctttt atcattgccc gttatgggtc gatatccgga gattacgcga
    10201 tgagaacgac ggttgttatt ctccgcaatc ctaatctcta agttaccgca tttattgaga
    10261 atttctcctt tttccctgtc gtttttctcc ataatttctt cataaaaaag ccgggctgca
    10321 taaaagcaaa cccggcctga ttgagataat gaatagattt tactgatgat tcatcatcaa
    10381 tttacgcaac gcagcaaaat cggcgggcag attatgtgaa agcaagggta aatcagcacg
    10441 ttctgccagc tcttttggca gatccaacgt ttcaccgaga atcgcttcca cgctctcttt
    10501 aaatttcgcc ggatgcgcgg tgccgaggaa caagccatat tcgcctggat tcaactggtc
    10561 acgcagcgca cgatacgcta cggcagcgtg cggctccgag gtgtagccca gttcttttaa
    10621 ctcacgcatt gtctgttgcg tggtttcatc atccacggct gcataaccca gctctttcag
    10681 ttgccagatt ttgcggcgga acaactcttc cacacgcggc cagttgttcg gctgactcac
    10741 gtccatcgcg ttggataacg tcgcctgagt cgctttgggt gaccactgac cgtcgtgcag
    10801 gaaacgtggc acggtatcgt tcacgttggt cgcagcaata aaacgtttca ccggcagacc
    10861 gaatgacttc gccagcagac ccgccgtcaa atcgccgaag tttccgcttg gcaccgagac
    10921 aaccagctgg ttgcgcgcct cctgcggcag ctgcgcaaca gcttcaaagt agtagcaaat
    10981 ctgcgccagc aaacggctga tgttaatcga gttagccgag tttaacccca gcgccacttt
    11041 cagttcttca tcatcaaacg cctgcttcac cagcgcctga caggcatcga aatcgccgtc
    11101 gatggcaaca gtttcgatat tgccgcccaa cgtacagaac agtttttctt gcagtggact
    11161 gattttgcct cgtggataga ggataaccac tttcacattc ggcaaaccgt agaaagcatg
    11221 agccactgca gctccggtat caccggaggt cgcggtcaga atggtcactg gcttatcatc
    11281 cgcaatatgg gtcagcattt gtgccataaa gcgaccgccg aaatctttaa atgccagcgt
    11341 tggcccgtgg aacaattcca gacaaccgac atcgctttca acattggcga ccggagccgg
    11401 gaaggcaaac gccgcgcgca cgcgctcttc caggatttcc tgtgggattt catcaccaat
    11461 aaacgccgag aggatcttcg cactgcgggt gacaaaatcc agcttcagca tctcatcaat
    11521 ttcagtcagg ctgaattccg gcaggtcgtg cggaaaaaac agcccctgat ttttgcccaa
    11581 cccctgggtt acggcttgcg caaagctgac ctgctcgttg tgatctttca gattgtagag
    11641 tttcatttag ttttccagta ctcgtgcgcc cgccgtatcc agccggcaaa tatgaacaaa
    11701 accttcctga ttttgcaggt agttcttacc caaccagtcg gcaacgcgct gggcggtatc
    11761 cggcttgtca catagagcga acaaggtcgg gccggagccg gagataccgc tcgctaccgc
    11821 gccgatttcc gcgaccgcct gccgcgcctg ccggaagcca ggcagtaacc gttcacggta
    11881 gggttcagcg ataacatctt tcatcagctt cgcggcaagc tcaggctgac gggaatagca
    11941 ggcgtgaatg aagccagcca gatgtcgccc gtgcgcaatg caatcctggc ggcgatactg
    12001 cgccggtaaa atagcccggg cttctgccgt cgagacttta attcccggat acgccagcac
    12061 ccacagccac tcatcaaacc ctggcacttg ctggctgatg atgtcgtttt cttcgatcat
    12121 caactgcata ccaccaagaa aacacggtgc cacgttgtcg taatgaatgc tgccggagat
    12181 acgtccttcc agctcgccca tcaaagccag caaacgagtg tcattaagcg gcttgccgca
    12241 gtgttcattc atcgccatca gcgccgcgac caccgaacag gcgctggagc ctaagcccga
    12301 accgatcggc atattctttt ccagagtcat cgccactgga atttgcttgc caagctcctg
    12361 gcaaaaacgc tcccagcact gataaacgat attttcccgt ggttctgacg gcagcttatc
    12421 ggcaaagcgt ccgaggttgt tgagactgaa tgtctctgcc gcctcaaccg tgactacatc
    12481 tccgagcaat gcaccatcaa cgggtgccac cgccgccccg agcacatcaa acccgacgct
    12541 catattggca ctggaagccg gggcataaac tttaaccatg tcagactcct aacttccatg
    12601 agagggtacg tagcagatcg gcaaagacac cggcagcggt aacgtcattg cccgcaccat
    12661 atccgcgcag caccaacggc agcggctgat aatagtggct ataaaaggcc agggcgtttt
    12721 cgccattttt cactttgaac agcggatcat tcccatccac ttcggcaatc ttcacgcggc
    12781 agacaccatc ttcatcaata ttgccaacat agcgcaaaac ttttccttca tcacgggcct
    12841 tcgccacgcg cgcggcaaag agatcgtcga gctgtgacag attcgccata aaagcggcaa
    12901 catcaccctc ggcgttaaac tctgcgggca gcacaggttc aatttcaata tccgccagct
    12961 ccagttcacg tcccgtttca cgagcgagaa tcaatagttt acgcgccaca tccataccag
    13021 aaagatcatc tcgcgggtcc ggttcggtat aacccatttc ccgcgccagc gtggtcgcct
    13081 cggagaaact catgccttcg tctaacttgc cgaagatata agaaagcgaa cctgaaagaa
    13141 tgccggagaa cttcaccaat tcatcacctg cattgagcag attttgcagg ttctcaataa
    13201 ccggtaatcc agccccaacg ttggtgtcat agaggaattt acgccgcgat ttttccgccg
    13261 cataacgcaa ctgatgatag taatccatcg acgaggtgtt ggcctttttg ttcggcgtga
    13321 caacgtggaa accttcgcgc aggaagtcgg catattgatc cgccactgcc tggctggagg
    13381 tgcagtcaac aatgaccggg ttcagcagat gatattcttt cacgaggcga attaagcgcc
    13441 cgagattaaa cggctctttg gcttgtgcaa gttcttcctg ccagttttcc agattaaggc
    13501 catgcacact agtgagcaga gccttcgagt tggcaacgcc gcagacacgt aagtcgatat
    13561 gtttattctt cagccagctt tgctgacgct tcagttgctc cagcagcgca ccgccaacgc
    13621 caccgacgcc aatcacaaac acttcgataa cctgatcggt attgaacagc atctgatgag
    13681 taacgcgcac gccagtggtc gcatcatcgt tatttaccac gacagagatt gagcgttcag
    13741 aagatccctg agcaatggcg acaatgttga tattggcgcg ggccagcgcg gcaaagaatt
    13801 tcgccgagat cccacgcaag gtgcgcatac catcacctac caccgagata atggccagcc
    13861 gttccgtcac cgccagcggc tccagtaagc cttctttcag ttccaggtag aactcttcct
    13921 gcattgcccg ttcagctcgc acacagtcgc tttgtggaac gcagaaactg atactgtatt
    13981 cggaagatga ttgcgtaatc agcaccacgg aaatacgggc gcgtgacatt gcagcaaaga
    14041 cgcgcgccgc catgccaacc atccctttca tccccgggcc ggaaacgctg aacattgcca
    14101 tgttattcag attggaaatg cccttgaccg gtaattcgtc ttcatcacgg ctggcaccaa
    14161 tgagcgtacc tggtgcttga ggatttccgg tatttttaat caggcaaggg atctggaact
    14221 gggcgatggg ggtaatggtg cgggggtgaa gaactttagc gccgaagtag gaaagctcca
    14281 tcgcttcctg gtaggacatc gacttcaaca acctcgcatc gggcacctga cgcgggtcgc
    14341 aggtatagac cccgtcaacg tccgtccaaa tctcgcaaca atcggcgcgt aaacaggcag
    14401 ccagcaccgc cgcggagtag tcggaaccgt tacgtccaag caccaccagt tcgccttttt
    14461 cattaccggc ggtgaaacct gccatcaaca ccatgtgatc agccggaatg cggcttgccg
    14521 caatacggcg ggtggactca gcaatatcga cggtagattc gaggtaatgc cccactgcca
    14581 gcagtttttc gaccggatcg ataacggtaa cgttgtgacc acgcgcttct aacacgccgg
    14641 ccataatggc gatcgacatt ttctcgccac ggcaaatcag cgcagcgttg atgctatccg
    14701 ggcactgccc caacaaacta atgccatcca ggacatgttt tatttgggca aattcctgat
    14761 cgacgaaagt tttcaattgc gccagcggga accccggctg ggcggcggcg agtcccgtca
    14821 aaagttcggc aaaaatacgt tcggcatcgc tgatattggg taaagcatcc tggccgctaa
    14881 tggttttttc aatcatcgcc accaggtggt tggtgatttt ggcgggggca gagaagacgg
    14941 tggccacctg cccctgcctg gcattgcttt ccagaatatc ggcaacacgc agaaaacgtt
    15001 ctgcatttgc cactgatgta ccgccgaact tcaacactcg catggttgtt acctcgttac
    15061 ctttggtcga aaaaaaagcc cgcactgtca ggtgcgggct tttttctgtg tttcctgtac
    15121 gcgtcagccc gcaccgttac ctgtggtaat ggtgatggtg gtggtaatgg tggtgctaat
    15181 gcgtttcatg gatgttgtgt actctgtaat ttttatctgt ctgtgcgcta tgcctatatt
    15241 ggttaaagta tttagtgacc taagtcaata aaattttaat ttactcacgg caggtaacca
    15301 gttcagaagc tgctatcaga cactcttttt ttaatccaca cagagacata ttgcccgttg
    15361 cagtcagaat gaaaagctga aaaatactta ctaaggcgtt ttttatttgg tgatattttt
    15421 ttcaatatca tgcagcaaac ggtgcaacat tgccgtgtct cgttgctcta aaagccccag
    15481 gcgttgctgt aaccagtcga ccagttttat gtcatctgcc actgccagag tcgtcagcaa
    15541 tgccatgact cgttcgcgta aagcttgcag ttgatgttgg tctgccgttg tgtcactttt
    15601 cgccggttgt tgtattaatg ttgctaattg atagcaatag accatcaccg cctgccccag
    15661 attgagcgaa ggataatccg ccaccatcgg cacaccagta agcacgtcag ccaacgctaa
    15721 ctcttcgtta gtcagcccgg aatcttcgcg accaaacacc agcgcggcat ggctcatcca
    15781 tgaagatttt tcctctaaca gcggcaccag ttctactggc gtggcgtagt aatgatattt
    15841 cgcccgacta cgcgcagtgg tggcgacagt gaaatcgaca tcgtgtaacg attcagccag
    15901 tgtcgggaaa actttaatat tatcaataat atcaccagat ccatgtgcga cccagcgggt
    15961 ggctggctcc aggtgtgcct gactatcgac aatccgcagt tcgctaaaac ccatcgtttt
    16021 cattgcccgc gccgctgccc caatattttc tgctctggcg ggtgcgacca ggataatcgt
    16081 tatacgcata ttgccactct tcttgaccaa ataaccgcga acagggtgat cactgtcaac
    16141 ttattacgcg gtgcgaattt acaaattctt aacgtaagcc gcagaaaaag ccctttactt
    16201 agcttaaaaa aggctaaact attttctgac tgcactaacg gtttaattgt taaaaaatgc
    16261 tacatatcct tctgtttact taggataatt ttataaaaaa taaatctcga caattggatt
    16321 caccacgttt attagttgta tgatgcaact agttggatta ttaaaattat gtgacgaaag
    16381 ctagcattta gatacgatga tttcatcaaa ctgttaacgt gctacaattg aacttgatat
    16441 atgtcaacga agcgtagttt tattgggtgt ccggcccctc ttagcctgtt atgttgctgt
    16501 taaaatggtt aggatgacag ccgtttttga cactgtcggg tcctgaggga aagtacccac
    16561 gaccaagcta atgatgttgt tgacgttgat ggaaagtgca tcaagaacgc aattacgtac
    16621 tttagtcatg ttacgccgat catgttaatt tgcagcatgc atcaggcagg tcagggactt
    16681 ttgtacttcc tgtttcgatt tagttggcaa tttaggtagc aaacatgcag accccgcaca
    16741 ttcttatcgt tgaagacgag ttggtaacac gcaacacgtt gaaaagtatt ttcgaagcgg
    16801 aaggctatga tgttttcgaa gcgacagatg gcgcggaaat gcatcagatc ctctctgaat
    16861 atgacatcaa cctggtgatc atggatatca atctgccggg taagaacggt cttctgttag
    16921 cgcgtgaact gcgcgagcag gcgaatgttg cgttgatgtt cctgactggc cgtgacaacg
    16981 aagtcgataa aattctcggc ctcgaaatcg gtgcagatga ctacatcacc aaaccgttca
    17041 acccgcgtga actgacgatt cgtgcacgca acctgctgtc ccgtaccatg aatctgggta
    17101 ctgtcagcga agaacgtcgt agcgttgaaa gctacaagtt caatggttgg gaactggaca
    17161 tcaacagccg ttcgttgatc ggccctgatg gcgagcagta caagctgccg cgcagcgagt
    17221 tccgcgccat gcttcacttc tgtgaaaacc caggcaaaat tcagtctcgt gctgaactgc
    17281 tgaagaaaat gaccggccgt gagctgaaac cgcacgaccg tactgtagac gtgacgatcc
    17341 gccgtattcg taaacatttc gaatctacgc cggatacgcc ggaaatcatc gccaccattc
    17401 acggtgaagg ttatcgcttc tgcggtgatc tggaagatta atcggcttta ccaccgtcaa
    17461 aaaaacggcg ctttttagcg ccgtttttat ttttcaacct tatttccaga tacgtaattc
    17521 atcgtccgtt gtaacttctt tactggcttt cattttcggc agtgaaaacg cataccagtc
    17581 gatattacga gtcacaaaca tcatgccggc cagcgccacc accagcacac tggttcccaa
    17641 caacagcgcg ctatcggcag agttgagcag tccccacatc actccatcca gcaacaacag
    17701 cgcgagggta aacaacatgc tattgcgcca gcctttcaat accgcctgca aataaatacc
    17761 gttcattaac gccccaatca gactggcgat tatccatgcc acggtaaaac cgatatgttc
    17821 agaaagcgcc agcaagagca aataaaacat caccaatgaa agccccacca gcaaatattg
    17881 cattgggtgt aaacgttgcg cggtgagcgt ttcaaaaaca aagaacgcca taaaagtcag
    17941 tgcaatcagc agaatggcgt acttagtcgc ccggtccgtt aattggtatt gatcggctgg
    18001 cgtcgtcact gcgacgctaa acgcggggaa gttttcccag ccggtatcat tgcctgaagc
    18061 aaaacgctca ccgagattat tagcaaacca gctgctttgc cattgcgcct gaaaacctga
    18121 ctcgctagct tcccgtttgg ctggtagaaa atcacctaaa aaactgggat gcggccagtt
    18181 gctggttaag gtcatttcgc tattacgccc ggcaggcacc acagaaagat cgcctgtacc
    18241 gcttaaattc agtgccatat tcagctgcag gttctgcttc cgccagtccc cttcgggtaa
    18301 agggatatgc acgccctgcc cgccttgctc taacccggta ccgggttcaa tggtcagtgc
    18361 cgttccgtta acttcaggcg ctttcaccac accaatacca cgcgcatccc cgacgctaat
    18421 cacaataaag ggcttgccta agatgatatt tggcgcgttg agctcgctaa gacgcgaaac
    18481 atcgaaatcg gcttttagcg ttaaatcact gtgccagacc tgaccggtat aaatccctat
    18541 cttgcgttct tccacgttct gattgccatc aaccatcaat gactcaggta accaaaaatg
    18601 gataaaactt cgtttccgct ccacggtttt atcctcttcc tgcaccgtat aaagctcggt
    18661 cacaggaata gcgataagcg gcccaacgag tttttgcggc ccgctggtac tttggcgaat
    18721 cgcatcttcc acatcgctac ggtaatcggc acgttcgaca atcacttgcc gaatcagcat
    18781 tatcggaatc aacaacagca atactgcacc aaacaaggtg gtcattttcc agaacagggg
    18841 ggatttcaac atagtcttct cctttgcaat ggcagcagga tacgaaggct atgtgggaag
    18901 aatttgaagc tatgtgaagt gacggtgaag tcgtagcgag gccagcacgc caccttcctg
    18961 cacgttgcgc agcgtgactt cgccgttaaa caaacgggcg acctcactga cgaacgccaa
    19021 ccccagaccg ctgctttttt gcccatttgc acgcggcaaa gagtaaaagc gttcaaaaat
    19081 acgtgacagc gcgtagtcag gaataccact accggtatcc agcaccttaa gcgtgacgtg
    19141 ttcctgatcc acttcggcgc ttagcgttat gcaaccgctc tcgggagtaa aatcgatggc
    19201 gttatccagt aaattcccca gcgcctgatc cagtaacgcc ggttcagcag caacattaac
    19261 ctcggagggc ataacatgca aagtgatgtt tttttctgcc aactgcacgg tgcgcgcttc
    19321 gctgacgcgg cgaaataatg ccgccacatc aacagcagtc agaacgactt cctgacgatt
    19381 ctccagtctt gcctggcgta gtaacgtttc caccagcgcc tgcattcgcg cattttgtgt
    19441 cagaatgttg tcagtaaaac gagccactac ttcaggcggc ggaccttcgc gtaaaatttc
    19501 cgccgcgcca cgaatcgccg ccagtgggct ttttagctca tgggttaacg cataaacata
    19561 ctgctcaata tagtttttcc cttccagctt cacgcgcata ctttccagcg cctgcgcgag
    19621 tttacgcagc tcgctactac cgagatcggg aagaggaacg ggcttattgt cagtgacgga
    19681 atcagcatag cgagtgagcc gagcaatgga gcggttgatc caccaaacca tgcctgcgcc
    19741 aatcaccagt gcaatcccca acaaaatggc gctggcccat aaaattcgcc gctcgctgcg
    19801 tttaatgacc ggagccatcg ccgcgttcgg tttgcctacg ctcaaaacgc caataagccg
    19861 cgagccgtcc ataatcggcg cggcaacata catcaccgaa ctttcgggat cggcaggatt
    19921 ttgcaacgtg ctgcgcgcac catactgacc acgcaacgtt agccagacgt cattccagcg
    19981 cgaataatcc tgtccaacgg ctttatttac cgaatcgaac aatactttgc catgcgcatc
    20041 ggtcatatag acatgatatt cattgcgcac tttgttaatg ccaccaatat tggcgcgaaa
    20101 cgggcgatgt tgtagctgat taaacgcctg cgccagttgc ccatgcgttg ggtccccaga
    20161 gagcaaatcg ggacgcgcca gctccgcaag taacgttgcc gtgtcgatca acgtcccctc
    20221 cgttgctctt cgcacgcccg gtttaacttc tttgacaaaa atagccagta cgaaccaggc
    20281 cgccaccgcc accagtaaaa aatagccccg cagcaaccgc atgccgatac gcattacaag
    20341 cccctcaggc tatatcccat gccgcgatga gtattaatcg gtgaaagatc ggggttgatg
    20401 gcgcgcagct tggcacgcag cgttttaatg tggatatcga cggtgcgatc gtaggtgtcc
    20461 tgcgcatctt cccacacgct atccattagt tgctggcggg accagacgcg gcctggcgac
    20521 ttgagtaacg tcttcagcaa taaaaactca taccgcgtca gcgttaatgg cgtgtcaaac
    20581 cagctgatct gcgccgcggg ttcattcaat tcaaaatgac caatacggat gacgggagac
    20641 ggcgtcgaga acttcttcac ccgacgcagt aaggtgcgca ccctggcgca cacttcgcgg
    20701 ggtgaaaacg gtttagccac atagtcgtca gcgccaattt ccagcccaag caggcgatcg
    20761 acctcttcac ttcgggccgt caggaacagt acaggtaacg ccggatggag cgccagtaac
    20821 tggcggcaca attcaaagcc actgatatca ggcaaaccaa cgtcgagaat catgacgtcg
    20881 ggaacctgct ggcgagcttt atccagcacc ggcaaacctc gctcaaagac ctcgacggca
    20941 aaaccttcct gctgcaacat gtagaccagc gtgtcggcta tcccttgctc atcttccact
    21001 aaccagaccg tttcccgttg cataaatcgc ctctgttatt gccgccacgg catgacagga
    21061 accgcgctaa tcgcgttttt cggcgaacct tctacaactt tgtcggagta agccagataa
    21121 gcgagcgcgt tgcgtttggc gtcataaaag cgcacgacct gtaacgactt aaagaccagg
    21181 gacgtgcgtt ttttgaatac tacctcgccc tgagctttgc cgtttttaat acgatccgac
    21241 agttcaatcg gcccgacttg ctgacaagaa atggccgcat cggaggtatc ttctgccagg
    21301 cccaatcccc ctttaatacc accggttttc gcccggctca cataacaggt gacatttttc
    21361 acatcgggat cgtcaaaggc ttccacaaca attttgtgat ccgggccgat cattttaaat
    21421 acggtgtcga ccgaaccaat ctcttctgca tgagccaatg gccccagcat tatcaggctt
    21481 aaagacagga tcaaatgctt gtatttcata ttgttaccat ttttatctat tacacgagca
    21541 attagttcgt cacggtaaat aacatattga ttaagaacaa aatctcaatt cttttatttc
    21601 cgtcaagccc taaaacatac tctactaaga aaaaaacact gaatgctaaa acagcaaaaa
    21661 atgctattat ccaattacct gatgtcaggt gctcgttgtt gaaaggatga ggatatttta
    21721 tggatcaggc cggcattatt cgcgaccttt taatctggct ggaaggtcat ctggatcagc
    21781 ccctgtcgct cgacaatgta gcggcgaaag caggttattc caagtggcac ttacagagaa
    21841 tgtttaaaga tgtcactggc catgctattg gcgcgtatat tcgtgctcgt cgtttgtcga
    21901 aatcggcggt cgcactacgc ctgactgcgc gtccgattct ggacatcgcg ctgcaatacc
    21961 gcttcgactc tcaacagaca tttacccgcg cattcaagaa gcagtttgcc cagactcctg
    22021 cactttaccg ccgttctcct gaatggagcg cctttggtat tcgcccgccg ctacgtctgg
    22081 gtgaattcac aatgccagag cacaaatttg tcaccctgga agatacgccg ctgattggtg
    22141 ttacccagag ctactcctgt tcgctggagc aaatctctga tttccgccat gaaatgcgtt
    22201 atcagttctg gcacgatttt ctcggcaacg cgccgaccat tccgccggtg ctctacggcc
    22261 tgaatgaaac gcgtccgagt caggataaag acgacgaaca agaggtattc tataccaccg
    22321 cgttagccca ggatcaggca gatggctatg tactgacggg gcatccggtg atgctgcagg
    22381 gcggcgaata tgtgatgttt acctatgaag gtctgggaac cggcgtgcag gagtttatcc
    22441 tgacggtata cggaacgtgc atgccaatgc tcaatctgac gcgccgtaaa ggtcaggata
    22501 ttgagcgata ctacccggca gaagacgcca aagcgggaga tcgcccaatt aatctgcgct
    22561 gtgaactgct gattcctatc cgccgttaac gctgcagctc atctaatgca ggggcgtcca
    22621 gatgcgagat gtcccctgcg gtttcaacga cccagccaga cgccagccac aggctttcct
    22681 gataatccac gcgcgaaata gaacagttac gcagacgtaa gcggcgctct gcccatgctg
    22741 gtaatccgag aatcgtactc accagacatc ccagtgcaat accatggctc accagcagcg
    22801 gtcggcttcc ctgcggtaag tcccggcaag attccagtgc cgcattgacg cgatcgctga
    22861 gctcctgcat tgactcgcct tcaggaatac gcccgtcaac ggtgccattg accagctgcc
    22921 gacgccagtt ctcttcttct tcggtcagag aatcgatatg tcttttttcc agcacaccca
    22981 tgtttaattc acgcaggcga gaatcaaaga tgatgtcaca gccgcaggcc tgggcgatga
    23041 tttccgccgt acgccgggtg cgtcctaaat cgctactgat aatatgcgta atgccaagct
    23101 ctttggcacg ggttgccacc tgcatcgcct gttgctcacc tttggcggtc agcgggctgt
    23161 cagactggcc ctgaatacgt cgctcggcgt tccactgcgt ttcaccgtgg cggactaggt
    23221 atacctgtaa catgcttttt ttccgtatac tgctgtaacg ttaattttct gagtaatgct
    23281 gattatgcac caagttgtct gtgcgaccac caatcccgct aaaattcagg ccattctgca
    23341 ggcatttcac gagatcttcg gcgaaggatc ctgccatatt gcatccgttg ccgtcgagag
    23401 cggtgtaccg gaacaaccct ttggcagtga ggaaacgcgc gctggcgcac gaaatcgggt
    23461 agcaaatgcc cgccgtttac ttccagaggc tgatttttgg gtggcgattg aagctggcat
    23521 tgatggcgac agcactttca gttgggtggt gattgaaaac gccagccagc gcggcgaggc
    23581 gcgttcggca actttgccgt tacctgcggt gattctggaa aaagttcgtg aaggcgaggc
    23641 gcttggtccc gtgatgtcgc gttataccgg tattgatgag attggtcgta aggaaggggc
    23701 gatcggcgtg tttaccgccg ggaaactcac tcgcgccagc gtctatcacc aggcggtgat
    23761 cctggcgctg agtccgtttc acaatgccgt gtatcaggca ttgtaggacg gataaggcgt
    23821 tcacgccgca tccgacattc ggtgcacgat gcctgatgcg acgctgccgc gtcttatcag
    23881 gcctacaaaa tcaatcgctt ttcagcaaca cctcttccag ccactggcgc agctccacgg
    23941 gcgcggcttt caggctgtta gatccacgcg taatcgtcgc gatgcccgcg ccgagttcat
    24001 tttttaactc acgctgactc atttcgccac gcaacagctc ttcgacaata cgcacgcgag
    24061 tccccaacgc ttcgcgctca tctggcgtca gcatcaggtt taacaacggt aaatggagat
    24121 cgttttggta ggcattctta agcaggtcga caaaacgtaa ccactcctgg tgacgctgtt
    24181 ctgccatcgc tgctgaatag ggtgattgtt gggccataat atgtcgccat tgttagcggg
    24241 ggaagcaaaa tgactccccc ggttgtactc gctaaagagt acgatagcat atcataaacg
    24301 tgcggatcag taacgacgtc cccattccgt ggcgctcatc aacgtcggtt tatcccccat
    24361 gaaatagcgg tagtaagcat cataagccag cacgttcttc acataaccgc gcgtctcgga
    24421 gaacggaata ctctcgacaa atgccacagc gtcgatacgc ccggcgctgt ggccaagcca
    24481 ggttcgcacc cgtccaggtc cggcgttata agctgctgag gagaaaatac ggttattgcc
    24541 aaactgctga taaacatatt gcaggtaact ggtgccaatg ttgatgttcg tttccggatc
    24601 cagcaattgc ccagggctgc tataaccagg aatagagaac atcttcaccg tatgggtcgc
    24661 tgtaccaggc ataatctgca tcaggccgct ggcccctacc ggtgatttca ctttcggatt
    24721 ccaggcgctc tcctgacggg caatcgccat cgcatagctt tgcgggatct ctttcccact
    24781 ggtgtaacgt ttgaaaagat cgttgtaagc cagcgggaat cgctcttcca gatgatccca
    24841 aagcttcccg gcgatcgttg cctgaacgct aagatcccac cattggttat tgaaagcata
    24901 ccgagccagt tgagcctgct ctgtttttga cttgctcttc accagattgg cccactcgct
    24961 acgcgcggta ttatcgagat tccagtacat caactcgcgc acgcgcgcca tctccggccc
    25021 ctgagtcagg gtgctgtcaa cattctgcgg cgctttatca atcttcagct catactcttc
    25081 accgatgcgt tgtgctgcaa ccatcgggta gaaaccacgc tgttgcatga gttgatgcaa
    25141 aatctctttc gcttcagctt cacgtccgcg ttccagcaat aaatccgcct gccagtaacg
    25201 ccattcatct ttctcttttg cttccatcgg cagacgcgcc agccaggtat tcaggccgcg
    25261 acgatcgccg gtgccaagcg ccattcgcac acggcgttca atcaacgacg tggattgcga
    25321 gcgcataatg gcgtcatcgc gccatttcgc ctgctcatcg gtgacatcat tgcccatcaa
    25381 acgccaggcg acgatatcgc gcagctcctg aatctgatct tcattaagct gctgcgcctg
    25441 ggcaagcgac gggatcatca gccgtgcatt ttcggcatcc tgccgcgcca cactggcaaa
    25501 cgccaccgcc gccatttgac gggtaaaatc ggtcgcgccg gttgtacgcg cgaaggtcag
    25561 taccgtatta gggttgttcg ccagtgaaat gattgccgag gcgatagtct ggtaatcggc
    25621 aggcatctgc cctgccagca ctgttactag gcctgtgtta cccgctttca tcgccagacg
    25681 gatacgctct aaatacgcca gcggatcttg tttacctgac gcacgccaga cgctaaataa
    25741 cttgtcacag gcgttaggct ggctcttgcc ggttagccac agctctttcg ccccttgcca
    25801 ggcttcttca ctctgcccgg tgttccattt cgcatagtag taattacatt gcgcttcggt
    25861 agttccgggc ttttccgggc taaaggctaa caagccacgc cagtcttcac gccgcgccag
    25921 ttcattgacg aaacgagatt gcagcgtgcg agcgggagga agcgtggggt tagcgcgaac
    25981 aaagttagtg accgtcaccg ccggttgatt catcaggtca tcggtgatct ggcggtattc
    26041 caggtaggga taaagcggat aatccttcag tccaggcatc atttgttcga ccacatccat
    26101 ctgtcgatta tcccaggcct gcttgatttg cgcgtaacgg ctacgctgct catccagtga
    26161 gtcggctcgc gccacgctgc tgaccgtcag cagacagaca ccggcagcca acagccgcca
    26221 ggtaacttgt ttggcttttt ccacaagcgc atcctctaag tgtaaataca tcaatgcagc
    26281 gtcatgccgc gtaatagatt tatgctaacc agtcattgcc gtttacgcca cgttacggac
    26341 acttttttac ttttactgcg aaggtgatcg accaactggg attaggcggg gaaacaggct
    26401 acacttcgcc tttgaaaact catcatcacg ataaacatag aggcgaagtc caacgtggct
    26461 caattcgttt ataccatgca tcgtgtcggc aaagttgttc cgccgaaacg tcatattttg
    26521 aaaaacatct ctctgagttt cttccctggg gcaaaaattg gtgtcctggg tctgaacggc
    26581 gcgggtaagt ccaccctgct gcgcattatg gcgggcattg ataaagacat cgaaggtgaa
    26641 gcgcgtccgc agccagacat caagattggt tatctgccgc aggaaccgca gctgaacccg
    26701 gaacacaccg tgcgtgagtc cattgaagaa gcggtttcag aagtggttaa cgccctgaaa
    26761 cgcctggatg aagtgtatgc gctgtacgcc gatccggatg ccgattttga caagctggcc
    26821 gctgaacaag gccgtctgga agagatcatt caggctcacg acggtcataa cctgaacgta
    26881 cagctggagc gtgcggcgga tgcgctgcgt ctgccagatt gggacgcgaa aatcgctaac
    26941 ctctccggtg gtgagcgtcg tcgcgtggcg ttgtgccgcc tgctgctgga aaaaccagac
    27001 atgctgctgc tcgacgaacc gaccaaccac ctggatgccg aatccgtggc gtggctggaa
    27061 cgcttcctgc acgacttcga aggcaccgtg gtggcgatta ctcacgaccg ttacttcctc
    27121 gataacgttg caggctggat cctcgaactg gaccgcggtg aaggtattcc gtgggaaggt
    27181 aactactcct cctggctgga gcagaaagat cagcgtctgg cgcaggaagc ttcacaagaa
    27241 gcggcgcgtc gtaagtcgat cgagaaagag ctggagtggg tacgtcaggg aactaaaggc
    27301 cgccagtcga aaggtaaagc acgtctggca cgctttgaag agctgaacag caccgaatac
    27361 cagaaacgta acgaaaccaa cgaactgttt attccacctg gaccgcgtct gggcgataaa
    27421 gtgctggaag tcagcaacct gcgtaaatcc tacggcgatc gcctgctgat tgatgacctg
    27481 agcttctcga tcccgaaagg ggcgatcgtc gggatcatcg gtccgaacgg cgcgggtaaa
    27541 tcgaccctgt tccgtatgat ctccggtcag gaacagccgg acagcggcac catcactttg
    27601 ggtgaaacgg tgaaactggc atcggttgat cagttccgtg actcaatgga taacagcaaa
    27661 accgtttggg aagaagtttc cggcgggctg gatattatga agatcggcaa caccgagatg
    27721 ccaagccgcg cctacgttgg ccgctttaac tttaaagggg ttgatcaggg taaacgtgtt
    27781 ggcgaacttt ccggtggtga gcgcggtcgt ctgcatctgg cgaagctgct gcaggttggc
    27841 ggcaacatgc tgctgctcga cgaaccaacc aacgacctgg atatcgaaac cctgcgcgcg
    27901 ctggaaaacg ccctgctgga gttcccgggc tgtgcgatgg ttatctcgca cgaccgttgg
    27961 ttcctcgacc gtatcgccac gcacatcctg gactaccagg atgaaggtaa agttgagttc
    28021 ttcgaaggta actttactga gtacgaagag tacaaaaaac gcacgctggg cgcagacgcg
    28081 ctggagccga agcgtatcaa gtacaagcgt attgcgaagt aatgcgtaaa atgccggatg
    28141 cggcgcgaac gccttatccg gcctacaaaa ccagcaattt caatacgttg taaaactgta
    28201 ggcctgataa gacgcggtaa gcgtcgcatc aggcattgag caccgaatgc cggatgcggc
    28261 gtgaacacct tttccggtct acaacagcct tagcatcctc aatttcgtgg cgggtcaccc
    28321 gtgcaggttt tttcaaacct tgtttaatct cgacagcctc ttccagagag gttttcaact
    28381 catcaaaaaa actcatttta tacctcgcct ttgagcagtt tcgtcacggt tatccttgct
    28441 cccccatcat ctcccgcacc agctccacgc agcgcaggaa acggctgtcg taatcgtcct
    28501 cttcaacccg cacgaattcg atattgttct cttccagcat ctccaccagc aagttctgga
    28561 actctttgcg atccaccgaa ctgccgaggc tgcgtaaacc atccgccacc cacggcgtgt
    28621 tgttctccag caggatcacc agatcgaaac ggtattcatc aatcagcgcc tgcacgaacg
    28681 gatgctcacg cccttcgtac tttttgcaga acgcctgggt agtgacaaaa tcggtgtcaa
    28741 taaacgccac tttattggcg tatttcactg caaaatcaat gtattgtgcg tggcccaacg
    28801 cgattttatc gtagtcggaa tactgcaatg cgatctcatc accgccgagg tgtgaaaaga
    28861 cataatcgcg gccatattcc cacgcactgg tggtgttgaa gatattggca agtttgttta
    28921 ccagggtgga tttaccgctc gactcgccgc caaggatcgc cacggtacgc acaaaaaacg
    28981 gcttcacttc ggtaggaata tattcccagt agcggaacgg gttttcgcgg atctgcgcac
    29041 cgctgatact cataaaggta cgtttcggat cgaccagcac cgtctcgatc cccagatgtt
    29101 ccatatactg tggcgcatcg gcttcttccg aggtgtagat caggtccggc tggatcccct
    29161 tttcagccat aaactttttg atgccgttgc tccacacatc ccagccatgc ggatacggct
    29221 ccatgccctc ttcgttgaaa gcatgaatac ggatattttt ctgatattta aaggtttgca
    29281 atagccagcg cagacgatcc ggcaccgtgg gctgctgcga catggcgctg tcttcaaaca
    29341 acgcgcggtc gcgggtatcg tcaaaaccca taatgatatg cagctcgtca acctggctac
    29401 aggcgcgctg gataaggtag atatgtccgg tatgcagcgg gtagaactta ccgaatacga
    29461 caccaatggt tttcttctgc cggggaaatt caagccccaa aaaacggtgc aatgcctcca
    29521 gcttttgcgc gctggggctt ttaattttgg cattcagtaa ctggcttaaa taccctttgg
    29581 tcataccgct ggcatcagct acctgctgta gcgtgcagcc ctgttgcttg atggcagttt
    29641 tcaggtaatc aaatgacgac atatctccct ccgtatctct cattataagt cgtcgaacac
    29701 gctaagcgcg tcggagagtt ttttaacgcc aaaaatctgc atcccttccg gcgctttttt
    29761 cggtacgtta gccgccggaa caatcgcccg gcgaaaaccg tgtttcgccg cttcagaaat
    29821 ccgctcctgg ccactgggca ccgggcggat ctcccctgcc agcccgactt caccaaacac
    29881 caccagatcc tgtggcagcg gtctgtcacg caggctggaa accatcgcca gcagtaacgc
    29941 taagtcggcg ctggtttcgg ttaccttcac gccgccgacc acgttcacaa acacatcctg
    30001 atcggccatt tgcagaccac cgtgacggtg caagacagcc agcaggattg ccagacggtt
    30061 ttgttccagc cccactgcca cgcggcgcgg attcgccatt atcgagtgat cgaccagcgc
    30121 ctgaatctcc accagcaacg gacgcgttcc ttcccacacc accatcacgg agctgccgga
    30181 ggtcacttca tctccgcgac ttaagaaaat tgccgaaggg ttgctgactt cacgcagccc
    30241 ctgttcggtc atcgcgaaga cgcccagctc attcaccgcg ccgaagcggt ttttatggct
    30301 gcgtaaggtg cgaaaacggg agtcggcatc gccatccaga agcaccgaac agtcgataca
    30361 gtgttccagc actttcgggc cagccagcga accatctttg gttacgtgtc caaccatgac
    30421 aatcgccaca ccgcgcgttt tggcgaaacg cgtcagataa gccgccgttt cacgcacctg
    30481 cgccacgcta ccaggcgatg attgtacatc cgccatatgc atcacctgga tcgagtcaat
    30541 taccatcagc ttcggttgct cttcttcggc aatcaggcag atctgctcga tgctggtttc
    30601 cgacaacata ttgagattgt cagtcggcag gccaaggcga tgagcgcgca ttgccacctg
    30661 ttgcagcgac tcttcgccgg tgacatacag cgttttcatc tgctgggcca gtttgcacag
    30721 cgtttgcagc aacagcgtgg atttccccgc accagggtta ccgccaatca gaatggcact
    30781 tcctggcacc acgccgccgc ctagtacgcg gtcgaactct ttaaatccgg tggaaaaacg
    30841 cggcagctct tcaaggctga tatcggagag tttctggact tttgccaccc cggcgctacc
    30901 ggcatagccg ctgagacgct cgttacgcgc caccgttggc gacgcagcaa gacgcacctc
    30961 ggtgatggtg ttccaggcat gacaggcact gcactgcccc tgccagcgcg gataatcggc
    31021 cccgcattca ttacaaacaa aggcgcgttt tggagctttt gccacggtgt acctcgttaa
    31081 tgctgtgccg cccgcaggat gacgggcagg caattacttc tgattcaggc tgccagagag
    31141 gatgcagaat acccccatca ggtcagcgtg acggatggtg acttccgcct tttcattcac
    31201 ttttggcttg gcatggtagg caatccccag ccctgctgct ttgatcatct gcaggtcatt
    31261 agctccatcg ccaatcgcca cggtctgcgc cagcgggatt tcatactcct gcgcgaggcg
    31321 agtcagagtt ttcgctttgt actgcgcgtc tacgatgtcg ccgatcacat tgccggtaaa
    31381 tttaccgtcc atgatctcca gttcattggc taccacggct gtcaggcgca gcttgtcgcg
    31441 caggtattca gcaaagaaag taaagccgcc ggaggcaatc gccactttcc agcccagcgt
    31501 ttccagcttg agcaccagct gcgttaagcc tggcatcagc ggcagatttt cacgcacctg
    31561 ttgcagaata ttggcgtcag cgcctttcag cgtcgccacg cggctgcgca ggctggcggt
    31621 aaaatcgagt tcgccgcgca tcgcccgttc ggttacctcc gccaccatct cgcccgttcc
    31681 ggccagcttg gcaatttcat caatacattc aatctggatg gcggtggaat ccatgtccat
    31741 caccagcaaa cccggcgtgc gcaggtgcgg gattttcccc agcggggcga catccagctg
    31801 cgcttcgtgg gccaggcgtg tagcccgtgc ggtgagtgaa cctgccagac gaatcacctg
    31861 ataatcttcc acgcaccagg cggcaacaat caccatcgcc gcacccagtt tgctctggta
    31921 ttgggtcaga cgctgtttat ccagcccacg accatacagc agccagccgc tacgacctgc
    31981 gtggtaatcc agtggcatca cttcatcacc acttaatgaa agaggcagac ccggccataa
    32041 agagacatct tcaggcaggt cgcaccaggt aatgttaggc attaaggctc cagtaaaatc
    32101 gttcgaagca gggaaaataa cgcatgaggc taccttgtat ccattgcttc tggcaacatt
    32161 aagtctcaaa ttttcaaagg gtggaagatg gctcgcacaa aactgaaatt ccggctacat
    32221 cgggcagtga ttgtcctgtt ctgtcttgcc ttgttagtgg cactgatgca gggggcgtca
    32281 tggtttagtc aaaaccacca gcgacagcgt aatccacagc tggaagaact ggcccgcacc
    32341 ctggcgcgtc aggtgacgct gaacgttgca ccgctgatgc gtaccgactc accggatgaa
    32401 aaacgcattc aggcgatcct cgatcagtta acggatgaaa gccgtatcct cgacgcgggt
    32461 gtgtatgacg aacaaggcga tcttatcgca cgttctggcg aaagcgtcga agtgcgcgat
    32521 cggctggcgc tcgacggtaa aaaagcaggc ggctatttta accagcagat tgtcgagcca
    32581 attgcgggta aaaacggacc gctcggctat ctgcgcctga cactcgacac ccatacgctc
    32641 gccaccgaag cccaacaggt ggataacacc actaacattt tacgcctgat gttgctgctc
    32701 tcactggcaa tcggcgtagt gctgacccgc acgctgctac agggtaaacg cacccgctgg
    32761 cagcaatcgc ccttcctgtt aaccgccagc aaaccggtgc cggaagagga agaaagcgag
    32821 aaaaaagagt gacccattac tacaagaaag gaaatcgtta tgtccacatt acgcctgctc
    32881 atctctgact cttacgaccc gtggtttaac ctggcggtgg aagagtgtat ttttcgccaa
    32941 atgcccgcca cgcagcgcgt tctgtttctc tggcgcaatg ccgacacggt agtaattggt
    33001 cgcgcgcaga acccgtggaa agagtgtaat acccggcgga tggaagaaga taacgtccgc
    33061 ctggcgcggc gcagtagcgg tggcggcgcg gtgttccacg atctcggcaa tacctgcttt
    33121 acctttatgg ctggcaagcc ggagtacgat aaaactatct ccacgtcgat tgtgctcaat
    33181 gcgctgaacg cgctcggcgt cagcgccgaa gcgtccggac gtaacgatct ggtggtgaaa
    33241 accgtcgaag gcgaccgcaa agtctcaggc tcggcctatc gcgaaaccaa agatcgcggc
    33301 ttccaccacg gcaccttgct actcaatgcc gacctcagcc gcctggcaaa ctatctcaat
    33361 ccggataaaa agaaactggc ggcgaaaggc attacttcag tgcgtttccg cgtgaccaac
    33421 ctcaccgagc tgctgccggg gctcacccat gagcaggttt gcgaggccat aaccgaggcc
    33481 tttttcgccc attatggcga gcgcgtggaa gcggaaatca tctccccgga caaaacgcca
    33541 gacttgccaa acttcgccga aacctttgcc cgtcagagta gctgagagtg gaacttcggt
    33601 caggctccgg cattctcgca tctgctggat gaacgcttta gctggggcgg cgtggaactg
    33661 catttcgacg ttgaaaaagg ccatatcacc cgcgcccagg tatttaccga cagcctcaac
    33721 cccgcgccgc tggaagccct cgccgggcga ctgcaaggct gcctgtaccg cgcggatatg
    33781 ctgcaacaag agtgcgaagc gctgttggtt gacttcccgg accaggaaaa agagctacgg
    33841 gagttgtcga cgtggatagc gggggcggta aggtaactcg ctgattaaaa tatccaagga
    33901 aggaaatatg atcgatctgg aaaaccagga acgggagatt atcaatttaa tgttcagcca
    33961 gcgtatttcc tggctagccg ccgtgcgtat caggcataaa ttatcgcttg ctgaagtaag
    34021 taaaatgtta ggtatctcga ttaattctct aaaacagatc gaaaagacgg aacggcttag
    34081 ctcaaacatc aaaagcaaaa tggcagaaat ttatggttgt ccaccagagt tgctgatatg
    34141 cccttcctgg atgacggctg aacataaatg aatagattcg tgaatagata cttgaatgta
    34201 ttttattttc tatccatatg aaaaataata gtaaatctat aaatcagttg ctattgatga
    34261 atagatacta aaaagccgga gcagtccccc actccggctt aacacgacaa tcgcctttca
    34321 gcgaaacaca attactcttt atcgcccagc agaacggatt ccagtgcgat tttgatcatg
    34381 tcgttgaagg tggtctgacg ctcagcggca gtggtctgct cgtgagtgcg gatgtggtca
    34441 gacacggtgc agatggtcag ggctttcgcg ccaaactctg cagccacgcc gtagatacca
    34501 gccgcttcca tttccacgcc gaggatgccg tatttttcca tcacgtcgaa catttcgccg
    34561 tccggagagt agaacaggtc agcggagaac aggttaccca cgcgagcatc aacgcccagt
    34621 gctttagctg catctactgc gttacgcacc atgtcaaagt cagcgatagc ggcaaagtca
    34681 tggtctttaa aacggatgcg gttaactttg gaatcggtgc aggcccccat accgataacg
    34741 acgtcgcgca gttttacgtg cggcagaact gcgccacagg aaccaacgcg gataattttc
    34801 ttcacgccga aatcggtgat cagttctttg gtgtagatgg agcaggacgg gatacccata
    34861 ccgtgaccca ttacggaaat tttgcggcct ttgtaagtac cagtgaagcc cagcataccg
    34921 cgaacgttgt tcacttcacg agcatcttca aggaaagttt cagcaatata cttcgcacgc
    34981 agcgggtcgc ctggcatcaa aactacgtca gcgaaatcgc ccatttctgc attaatgtgt
    35041 ggggtagcca ttgttttatc ctttttgtga cataacaaag gcggagtcaa cgctccgccc
    35101 aaatccatca gaacatggct ttgccatatt ccatatcaga agtaccaaaa tattttgcca
    35161 gagtctggcc gatatccgcg aaggtttcac ggtgacccag tgagcccggt tttactttcg
    35221 ggccatacac cagtaccgga atgtgttcac gcgtgtggtc agtaccggtc caggtcggat
    35281 cgcaaccgtg gtcagcggtg aggatcagga tgtcgtcatc gcgcagcaga gacatcagct
    35341 ccggcagacg gcggtcgaac agttccagac ccgcagcata accggcgacg tcgcgacggt
    35401 ggccccagga agagtcgaag tcaacgaagt tggtgaagac gatggtgtta tcacccgctt
    35461 ctttcatctc tttgatggtg gcgtcaaaca gcgcgtccaa gccagtcgct ttcacttttt
    35521 tggtgatacc gcagttggcg tagatgtctg caattttacc gacagaaacc acctggccgt
    35581 gtttttcatc aaccagtttc tgcagcacgg tcggtgctgg cggctcaaca gccaggtcgt
    35641 gacggttacc ggtacgctgg aagttaccag ctttgtcacc gataaacgga cgagcgataa
    35701 cacgaccgat attgtagccg ccgttggtca gctcttcacg ggcgatttcg cacagttcgt
    35761 agagtttatc cagaccgaaa gtttcttcat ggcaggcaat ctggaacacg gagtcagcgg
    35821 aggtatagaa aatcggcttg ccggttttca tgtgctcttc gcccagttga tccagaatga
    35881 ccgtaccgga agagtggcag ttaccgaggt aacccggcag attagcgcgt tcgaccagtt
    35941 tatccagcag ctcttgcggg aagctgtttt cgtgatcgga gaaatatccc cactcaaaca
    36001 gaaccgggac accggcgatt tcccagtgac cagacggggt atctttaccg gatgacattt
    36061 cgtgcgccca tgcgtacgcg ccgataactt cagcgttgcc gtccattccc gccggaatga
    36121 aaccggtaga accttcgtgt gctttcgcca gccccagacg ggtcagattt ggcagattga
    36181 gcggcccttt acgaccatta tcagcttcgc ctttggcaca agcttctgcg atatgaccca
    36241 gggtgtcagc cccgacgtca ccaaagcgtt ctgcatcttc tgtagcgccg atgccgaatg
    36301 agtccagcac cataataaat gcacgtttca tatgttctcc gtactcagtg cttcaaattt
    36361 aaatgatcag atcagtatac cgttattcgc tgatacggcg atagacagtt ggtgtgcttt
    36421 ccggtgcttt atcggcaagt ttaattgccg ctttcaccgc tttcgccgct tcctgccagc
    36481 tgttttcgtc tttcgctagg ataaccgcca gcggacgctg accgtctacc tggtcgccca
    36541 gacgcgccat atcagtaaag ccgacgctgt aatcgatggt gtcagatgcc tgacgacgac
    36601 cgccgcccat tgcaaccact gccatcccca gcgcgcgggt atccatttca ctgacaaaac
    36661 cttcggtatc agcatagact gctttcgtca gcatcgctgt cggcagatac ttcgcgtagt
    36721 tctcaacgaa gtcggtcggg cctttttgtg ccgctaccat acgaccaaag acttctgccg
    36781 ctttaccgtt gtccagcacc gcctgcaatt tcgcgcgcgc ttcggcgtca tctttcgcca
    36841 gtttgccgga gatcagcatc tccacgcaca gcgccatcgt gacatcaaac agacgcgggt
    36901 tacgatactc acccgtcagg aactgcaccg cttcacgaac ttcaaccgcg ttacctgcac
    36961 tggaggccag tacctgattc atgtcggtga gcagcgcggt ggtgcgcacg ccagcgccgt
    37021 tagccacgcc aacaatcgct tcggcaaggg cttcagagag ttcgtaggtc ggcataaacg
    37081 cgccgctacc cactttcacg tccatcacca gcgcgtccag accttccgcc agtttcttcg
    37141 ccaggatcga ggcagtgatc agcgggatgg agtccacggt tgcggtaata tcacgggtcg
    37201 cgtagaaacg tttatcagcc ggagccagtg aactggtctg accgataatc gccacgccga
    37261 cgtctttaat aatttcgcgg aaacggttgt catccgggaa aatgtcgaag ccagggatgg
    37321 attccagttt gtcgagcgta ccgccagtat gaccgaggcc gcgaccggag atcatcggaa
    37381 tatagccgcc gcaggctgcg accatcggcc ccaacatcag cgaagtgaca tcgccgacgc
    37441 cgccggtcga gtgtttatca acaatcgggc cattcagatg caggcttttc cagtcgagaa
    37501 cggttcctga atctcgcatc gccatggtca gcgagacacg ctcaggcatt gtcatatcgt
    37561 ggaagaaaat ggtcatcgcg agggcggcaa tctgcccttc ggaaatagtg ttgtcgcgaa
    37621 taccgttgat aaagaaacga atttcttcat cgctcagcgc atgaccatca cgttttttac
    37681 gaataatttc ttgtgcgaga aacaaggtaa cctcccgagg aaaagagtat gaggcggctt
    37741 ttgccggatg cgtctacgcc ttatccggcc tacgggtaga tacgtctcgt aggcctgata
    37801 agcgcagcgc atcaggcgta aagcatctta cttagtagct gctggcgctc ttaccgtcgc
    37861 cgtggcccag cgctttcagc aggcttgcca gcaggctgga agcaccaaag cggtagtgac
    37921 gcgcatcagc ccagtcagca ccgaacagtt catctgcgat ggcgagatat ttctgcgcat
    37981 cttccgcagt acgcacgccg cccgccggtt tgaaaccaac ggttttttct acgcccatat
    38041 cacggatcac ttccatcatg atgcgagcgc tttccggcgt cgcgttcaca gccactttac
    38101 cggtagaggt tttgatgaag tccgcacccg ctttgatgga gatttcagac gctttacgga
    38161 tcagcgcttc gtctttcagt tcgccggttt cgatgatcac tttcagcagt acattcgctg
    38221 ccgcgcaagc ctctttacag gctttcacta ggtcaaaacc aacctgctcg ttacccgcca
    38281 tcagcgcgcg gtacgggaac accacgtcaa cttcatcagc accgtaggcg attgccgcac
    38341 gggtttctgc cagcgcgatt tcgatgtcgt cgttaccgtg tgggaagttg gttaccgtag
    38401 cgatacggat ttccggggtg ccctgctctt tcagagtttt gcgagcaatc gggataaaac
    38461 gaggatagat acagatagcg gcggtattgc cgaccggagt tttggcctga tgacacagag
    38521 caattacttt ctcgtcggtg tcgtcgtcat tcagggtggt caggtccatc aatttcagtg
    38581 cacgcaggct gcttgctttc agatcagtca tttcattctc ctggcttgtc gccaataaaa
    38641 ttcaccttgc gagtttgtta gtattctaac atctactccg caacacactt cgatacacat
    38701 cacaattaag gaaatctact tacaagtttg catcactgta atgcgatctg gttcaaataa
    38761 ttcactttca aatgaatgcg tcagtggtgg caaacgcatc aggatcaaag tgaacatcac
    38821 gaaacttctt acaatggcgc atcaccttcg gcataaaagg aaaagaagat gcccacttct
    38881 catgaaaatg cactgcaaca acgttgccag caaattgtca ccagcccagt gcttagcccg
    38941 gagcagaagc gccattttct ggcactggaa gcagaaaaca atctgcctta cccacagctt
    39001 cctgccgaag cccgccgcgc gctggatgaa ggtgtaatct gcgatatgtt tgaaggtcat
    39061 gcgccgtaca aaccgcgcta tgtcttaccc gattacgctc gttttctggc gaacggttcc
    39121 gaatggctgg agctggaagg cgcgaaagat cttgatgacg cactctctct gctgaccatt
    39181 ctttaccacc acgtaccgtc ggtcacatcg atgccggtct acctggggca actggatgcg
    39241 ttgttgcaac cgtatgttag aattctaaca caaaacgaga tcgatgttcg aataaaacgt
    39301 ttctggcgtt acctcgacag aaccctgcca gatgccttta tgcacgccaa tatcggcccg
    39361 tctgattcgc ccattacccg cgcgatctta cgtgcagacg cgcaggtttc accgaacctg
    39421 acctttatct acgatcctga aatcacccct gatgacctgc tgctggaagt ggcgaagaac
    39481 atctgtgaat gtagcaaacc gcacatcgcc aacggtccgg tgcatgataa aattttcaca
    39541 aaagggggct acgggattgt gagctgttac aactcactgc cgctggcggg tggtggcagc
    39601 acgctggtac gccttaacct gaaagccatt gccgagcgca gcgaatcgct ggatgacttc
    39661 tttacgcgca ctctaccgca ctactgccag cagcagatcg ccatcatcga tgcgcggtgt
    39721 gaattcctct atcaacaatc acacttcttt gagaatagct tcctggtgaa agaagggctg
    39781 attaaccctg aacgttttgt gccaatgttt ggcatgtatg ggctggcgga agcggttaac
    39841 ttgctgtgtg agaaagaagg gattgccgcg cgctacggta aagaagccgc cgcaaatgaa
    39901 gtgggttatc gcatcagcgc gcaactggcg gagtttgtcg ccaatacccc cgtgaaatat
    39961 ggctggcaaa aacgcgccat gttacacgca cagtcgggga tcagttccga tatcggcacc
    40021 acgccgggcg cgcgtttgcc gtatggcgat gagccagatc cgatcaccca tctgcaaact
    40081 gtcgccccgc atcatgctta ttattattcc ggcatcagcg acattctgac gctcgacgaa
    40141 atcatcaaac gtaacccgca ggcactggta cagctttgcc tcggtgcctt taaagccgga
    40201 atgcgtgaat ttaccgccaa tgtcagcggt aacgatctgg ttcgcgttac cggttatatg
    40261 gtgcgtttgt cggatttaga aaaatatcgc gccgaaggtt cacgcaccaa caccacctgg
    40321 ctgggcgaag aagccgcacg caacactcgt attctggaac gccagccgcg cgtgataagc
    40381 catgaacagc agatgcgctt tagtcagtaa gattatcccc ttctcctgcg ttgacgggcc
    40441 aggcagtcgt ctggctctgt ttttgcaggg ctgcaatctg cgctgcaaaa actgtcacaa
    40501 tccgtggacg atgggacgtt gcaatggctg tggggagtgc gtgccacagt gtccgcatca
    40561 ggcgttgcag attgttgacg gcaaagtggt gtggaacgct gtggtctgcg agcaatgtga
    40621 tacctgcctg aagatgtgtc cgcaacatgc cacaccaatg gcgcaatcca tgagcgtgga
    40681 cgaagtgctt agccatgtgc gcaaagcggt gctgtttatt gaagggataa cggtaagcgg
    40741 cggcgaagcc acgacccaac tgccgtttgt agtggcgctg tttactgcta tcaaaaacga
    40801 tccgcaactg cgccatctca cctgtctggt ggacagtaac ggtatgttga gcgaaaccgg
    40861 ctgggaaaaa ttactcccgg tgtgtgacgg cgcaatgctc gatctcaaag catggggtag
    40921 cgaatgtcat caacaattaa ccggacgcga taatcaacag attaagcgca gcatctattt
    40981 gctggcagag cgcggcaagc tggcggaact gcgtttgctg atgatcccag gccaggtgga
    41041 ttatttgcaa cacatcgaag aactggcggc gtttatcaag ggacttggcg atgttccggt
    41101 tcgcctgaac gcgtttcatg cccacggcgt gtatggcgag gcgcaaagct gggcgagcgc
    41161 cacgccggaa gacgttgagc cgttggctga tgcattaaag gtgcgcgggg tgagccggtt
    41221 gatatttccg gcgctctatt tgtgacatgc cggatacggc ctacggttcg gcacagactt
    41281 gtaggcctga taagacgcgt caagcgtcgc atcaggcatt gtgcgccaac tgccggatgc
    41341 ggcgtgaacg ccttatccgg cctacggttc ggcacaaacc tgtaggcctg ataagacgcg
    41401 ttaagcgtcg catcaggcat tatgcgccga ctgccggatg ctgcctacga agggcaaact
    41461 acccacgaac gttaaacacc gcatacgtgt tattaagcaa cacttcggca atctcatccg
    41521 cgggttccgg gcgtaactcg caaagcacgg caaacacacg ggcagcctgt tccgggcggt
    41581 taggctgccc ctgaaaaccg ttgagcggca tatccggcgc atcggtttcc agcaacaacg
    41641 atgccagcgg taattttgcg atgacatcgc gggttttact ggcgcgtgga taggtgatag
    41701 taccgcctac gccgattttg tagcccagct gtacaaaccg ttcagcctgt tgctggctgc
    41761 cagaaaatcc gtggacaaca ccagcacgcg gcaaatcgtg gcgtttaaga tgcatcgcca
    41821 gtttgtcgtg cgtgcgccgt gaatgcagga tcaccggcag atcgtagcgt ttcgccagtt
    41881 tcagttgttc gtcgagtaac cactgctgcc tctcaaattg cggatcgtcg ccgaagagat
    41941 ccagaccgat ctcccccacc gccaccacct tcgccggacg cctttccagc gcctgctgta
    42001 gctgctcaag agacacatcg ctatgttttt ccaacatacc aggatgcaag cccaatgcgg
    42061 catacagcgg ttgataattt tccgctaacg cctgcacgcg ggcaaaattt tccgcctctg
    42121 tcgccggaac aatgatcttg cctacgcccg cttgtgccgc gcgttgcagg ctggcctctt
    42181 catcgccaat aaaaggcggg aaatcaaaat ggcagtgagt gtcgataaaa cggcaaatca
    42241 agccagatcc tcattgttaa atgtcgggtc gttcgcctgc ggagcgtcgc tcacttcggc
    42301 aaccagcgta tcgttggcga ctggcgcagg cggaatgaca atcgattccg gcgtcaccac
    42361 tggcaccaga tggcgggtaa gcggcgcttt ttcagttaat agcttgccaa ccgtggcgag
    42421 gaaataacga ccgcataaac gcccaagttt atagtcttca cgcagcgccg gaatccgact
    42481 gccaagcgcg atactatgta atggcttcgg cggataaatt tcgaatatcc gcagcttgcc
    42541 cggtggtttc tcaataaatt gctgaatgtc acgatagctg gtttcatgat gctgcaccag
    42601 attgaccagc ggctgcaggc tactgtcacc cagccagcgt tccatgcgtt tgaaccactg
    42661 cggcgtgtag tacatttgtg acggcacagt gcggatgacg accaacgttt tagcgccctg
    42721 ccttgccgcc tctttaaccg gaatcgcatc actgatcccg ccatccaggt agttaatgcc
    42781 ttccagcgac actccgctac gataaaagcc aggtatcgca ctggaggcgc gaatcacatc
    42841 cagccagttt tgtttggttg gtaaaaagta attcggcgcg tagtcatcct gacgacaggc
    42901 gcacatataa aacgatttgc cgctgtcaaa caaccgcgcg gcggtatcca tttgcaacgg
    42961 catctggctt gcggtggcct ccaccagcca gtcgagatcg ataagatttc ctccacggac
    43021 aaagcgcaat ggatcgaaaa attcgcgttt tgtggtatag cgcatgatga ctttgcgcgc
    43081 gtaaccgggc tgattgcaga taaacgccga gaggttctgc gccccggcag atgtgccgag
    43141 ataaagatcg aaaggattaa actgcgcgcg cataaactca tccagcacgc cagccgtgaa
    43201 aattccacgc tgtccgccgc cttcgcacac cagagctatt cgtccaggct ggaacggcct
    43261 tagcgacaac ggcgcaatat taccaagcgt tacaggtatt cgctgcccca cttctgcctt
    43321 cctgtttttg ttgtgaaaga aagacaaaat aacgcaattt attattcgct aaaaccgcaa
    43381 aagccagtcc gctggactgg ctttggataa aattttctta atggtctgaa atctagggtc
    43441 gttttcggcc catgaacaaa ctcaccagga acagaataat cccgacgaca aagacaattt
    43501 tagctgcgcc tgcagcggta ccggccagac caccaaaccc aagtgcggcg gcgattaacg
    43561 cgataaccag aaatatgatg ccccaacgaa acataagcgt ctcctttacc atagttaatg
    43621 tcaccgcttg atatgagcgt gaaaatcact caatgcgata tttttagtgt ggtgcacatc
    43681 gcgcctcccg acaaagttcg ggaggacgaa ttacgacgaa ttacttagtt ttcagatcat
    43741 ttttaacgct tttcacacca tctaccgctt tggcgatact ttcagcacgg tcactttgtg
    43801 cctgagaatc tacggtaccg gagagctgaa ccacgccatc ggtggtttca actttcactt
    43861 tacgtgaagg gacgatatcg tccgccagca gtttggcttt gatttcactg gtggtggcgg
    43921 tgtcacccgc gtagcccttc accgagcctt ctttagcgtc gcgaacgtgc agtttgtcgc
    43981 tgacagaggt cacgccttca acgcctttcg ccactttcac tgcctcttcg gcctgggcct
    44041 gactttcaac gaaaccgctc agggtcacga ctttttgatc ggttttgaca gagatatcgg
    44101 tgctcttgat gttgtcatga tccaccagcg ccgccttcac tttcgcggtg atggcgctgt
    44161 catccatgaa attaccgact ttattcatag agctatcgac tttttgccct gcgctttcat
    44221 tggtagtctg cgcgttgttt tccgcgtagg cagagccggt cgcgacggca gaggtcaaca
    44281 ttacagccag cagagttttc gaaatcttca gtcttgtcat agtcatcgat ttattcctgt
    44341 atgtttgctc gtaatttgag cctggcaaca cgaggttgca ttgctgaata gggagagact
    44401 tcaccctcta cagaagtcaa tggtcgccat cacaaaagcg atgggtgatg aataacgacc
    44461 attacagcct ctgaatcagt tattaatatc ggtagaatga caatcgacgg ctttagatac
    44521 tgatatctac gcattgaacg gtatttaacg ccatcagaaa tgtcatcact ttgttaaata
    44581 tagatcacaa ttttgaaacc gctcgggata tcagcgaaaa cataagcaaa agtgaatgtt
    44641 ttaagaacat tccgtaagcg gctaataagg aagggaaatt gacagggcgc agcggctacc
    44701 gcgccctgga ggcaagaatt aatgctcacg ggtctggtgg aactgaacgt ccggataacg
    44761 ttcctgtgcc aggcgcaggt tgaccatgct ggtagcgatg taagcgaggt tatcgccgcc
    44821 atcaagcgcc agttggcttt cgttcttacg cttgaactct tcgaatttct tcgcgtctgc
    44881 acattctacc cagcgggcag tggcaacgtt gactgactca tacactgcct caacgttgta
    44941 ttcgctcttc aggcgcgcta ccaccacatc aaactgcagc acaccaaccg caccaacgat
    45001 cagatcgttg ttggagattg gacggaacac ctgcaccgcg ccctcttcgg aaagctgtac
    45061 cagccctttg agcagctgtt tttgcttcag cggatctttc aggcggatac gacggaacag
    45121 ttctggtgcg aagttcggaa taccggtgaa cttcatcatc tcaccctggg taaaggtgtc
    45181 gccgatctga atggtgccgt ggttgtgcag gccgaggata tcgcccggat acgcttcttc
    45241 aacgtgcgaa cggtcacccg ccataaaggt cagcgcgtcg gagatcacca catctttcgc
    45301 agtgcgcacc tggcgcagtt tcatgccttt ttcatattta ccggacacca cacgcataaa
    45361 cgccacgcgg tcgcggtgtt tcgggtccat gttggcctga attttaaata cgaagccggt
    45421 aaatttatct tcgctcgctt ctacggtacg ggtatcggtc tgacgcggca tcggcgcagg
    45481 cgcccactcc accaggccat ccagcatatg atcaacgccg aagttaccca gcgcagtacc
    45541 gaagaatacc ggagtgattt cgcccgcaag gaacagctct ttgtcgaact cgttagacgc
    45601 gcctttaacc agttccagtt cgtcacgcag ctgctgtgcc agatcttcac caaccgcagc
    45661 atcgagatcc gggttattca gccctttaac aatgcggact tcctgaatgg tgtgcccttt
    45721 accgctctga tagagatagg tttcatcttt ataaaggtgg taaacgcctt taaacagctt
    45781 gccgcagcca attggccagg tgatcggtgc gcagccaatt ttcagctcgt tctcaacttc
    45841 atcgagcagt tccatcgggt cgcggatatc acggtcaagt ttgttcataa aggtgaggat
    45901 cggcgtgtcg cgcagacggg taacttccat cagcttacgg gtacgatctt caacaccttt
    45961 tgcggcgtcg ataaccatca ggcagcagtc caccgccgtc agggtacgat aggtatcttc
    46021 cgagaagtct tcgtgccccg gggtgtcgag caggttaacc aggcaatcgt gatacggaaa
    46081 ctgcatcaca gacgtagtaa tggagatccc acgctgcttt tccatctcca tccagtccga
    46141 cttagcgtgc tggttggaac cacggccttt tactgtaccg gcggtctgaa tggcctgtcc
    46201 gaacagcagc accttctcgg tgatggtagt tttaccggcg tccgggtgag aaataatggc
    46261 aaaagtgcgg cgcttcgcca cctcttgcaa ataaggagac aacgtcataa ttctttcttc
    46321 ttgagtaagc ggcatcgcgc cgcgcttgtt gaaaacgaaa aattgcggct attttaccca
    46381 tcaacggggg ggaggcaatc agtgtttaca caggagctgc tccagttcgt gcaacgaaga
    46441 aacggtccag gtgggcgcga tgccttctgg ttgctcgcga ttgtgcgcat tcagccagca
    46501 ggtcgcaagc ccggcgttga tgccaccgag aatatcggac tcggcagtgt cgccaaccat
    46561 cagcacgcgt gaacggtcag gattgcccgc ctgttccagc gcataatcga aaattttctt
    46621 attcggtttg gcaacgccaa cttcttcgga aatcaccagc aaatcgaagt aatcacgcag
    46681 gcccgtccgt tccagacgca cttgctgcaa ggcgctaaaa ccgttggtga tgatgccgat
    46741 tttagcgttg ccacgaatgg cgttaagcag agaaaccgcg cccggcaacg gcgtacagat
    46801 ttccgccatc gcattaataa aggcatcgtt aagcaagcct gcttcaactt tcagccgttc
    46861 ggcccagctc tcgaaacgcc cgtgctgaag ctgtaatgaa gtgatcgcgc cgttttgata
    46921 atccacccac agtggcttgt taacggcctg atagtcctga aaatcttcag cggtaaaggt
    46981 gacgctgtaa tcaagaaaca tccgctgcag gccggtgaat gagtcaaagg taaacagcgt
    47041 ttcatcggca tcaaagaaaa tccagtccca cttcattatt ccaccttgtc ttacatactg
    47101 attggcaacg ccatgatgat ggcgtcttcg cgaccgtccg tggtggggta gtaattgcgg
    47161 cgaatcgtcg cctcgttaaa gcctaaactt tcgtacaggg caatggcggc agcatttgag
    47221 gcacggactt ccagccatag tgtcgccacg ccgcgttttt ccagttcgtc gatcagatgt
    47281 tccagcagcg ccctacccag cccctgacgc tgatagtcgg gatcgaccgc aatattgaac
    47341 aatgtagctt catccagcac cacttgcgta atcgcaaacg ccgccatttt gccgttttgc
    47401 gttaactgaa agttgagata acgctcgccc tggttgctgg caaaggtttt ttcactccac
    47461 ggaaaggcgt gggcgcgttg ttcaatgtgg taagccgctg gtaaatcagt cgtttcgagg
    47521 gaagaaatcg tgttcatatg tgcaaatttg ttgccataac gcggcgcgtg ccgttgggtt
    47581 tgcccgtaat tcggtgagcg ccggtgatgc cacctgagcg ccttccaatg atagcggttc
    47641 gtcagtaccc aaccgccagc tgttgcagcg actgccttgc ggcagcatcg cgattttttc
    47701 tggcgtcagt tgcagcactt gatcggggct gacggttaat gcgcgcagaa catcgctcac
    47761 taaaggatca gtcagggcgg gaagatcgtt tgccaccatc accagacgga cgtgtgccgg
    47821 gctcgcaatg gcaatctccc cctgcaacgc gccagggcga cgcagcgacc actgggtaat
    47881 gcccagttgc tgtaactgcc agtctcgtcg ggatgtcata gcgaatcgct cctgttgtca
    47941 ggggcgcaaa tatagcaaat tcgtcgatac cgcgccaaca tatggctata atcgccgcca
    48001 gtatcaattg aggagcattc catgtctgca tttaccccgg caagtgaagt cttgctgcgt
    48061 cacagtgatg atttcgaaca aagccgtatt ctgtttgccg gagacttaca ggatgacctg
    48121 cccgcgcgtt tagataccgc ggccagccgt gctcataccc agcaattcca ccattggcag
    48181 gtgttaagct gccagatggg ggataacgcc cgttttagtc tggtcgccac ggcgaatgat
    48241 gtcgcagatt gcgatacgct gatttactac tggccgaaga acaaaccgga agcccagttc
    48301 cagttgatga atttactttc tctgctgcca gtggggacag atatttttgt cgttggcgag
    48361 aaccgcagcg gcgtgcgcag cgctgagcag atgctggcag attatgcgcc gttgaataaa
    48421 gtcgacagcg ctcgtcgctg tggcctctat tttggtcgtc tggaaaaaca gccagtattt
    48481 gatgcggata aattctgggg cgaatacagc gtcgatggcc tgacggtcaa aacgctgcct
    48541 ggcgtgttta gccgcgacgg tctggatgtc ggtagccagt tgctgctctc gacgttaact
    48601 ccgcacacga aaggtaaagt gctggatgtc ggctgtggcg cgggtgtact ttcagttgcc
    48661 tttgcgcgcc attcgccgaa aattcgtctc accttgtgcg atgtctctgc cccggcggta
    48721 gaagccagcc gcgcaacact tgcagccaac ggtgttgaag gtgaagtctt tgccagcaac
    48781 gtcttttccg aggtgaaagg tcgttttgat atgatcatct ccaacccgcc gttccacgat
    48841 gggatgcaaa ccagcctgga tgcggcgcaa acgctgattc gcggcgcggt gcgtcatctt
    48901 aatagcggcg gcgagctgcg aattgtagcg aacgccttcc tgccttaccc ggacgtgctg
    48961 gatgagacat ttggcttcca tgaagttatc gcgcaaaccg ggcgcttcaa ggtatatcgc
    49021 gccattatga cccgccaggc gaagaaaggt tgagtatctg atatctgtcg ccgcataccg
    49081 tgtcggatgc ggcgtgaacg ccttatccga cctacaaaac ctgcacgtta gcccttcgta
    49141 ggccagataa gacgcgccag cgtcgcatct ggcatctgcc cccgcatgcc taccaaacct
    49201 gcacgctagt ttcctgatgg acatttttcc agcaattaca catcaggcga taattaacta
    49261 ttgacgaaaa gctgaaaacc actagaatgc gcctccgtgg tagcaattct ttttaagaat
    49321 tgatgg
//
LOCUS       DADLSA010000013        48347 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.013, whole genome shotgun sequence.
ACCESSION   DADLSA010000013 DADLSA010000000
VERSION     DADLSA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.013"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..209)
                     /locus_tag="JN691_000776"
     CDS             complement(<1..209)
                     /locus_tag="JN691_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7242290.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JN691_000777"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JN691_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAY7242291.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JN691_000778"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JN691_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAY7242292.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JN691_000779"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JN691_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAY7242293.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JN691_000780"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JN691_000780"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAY7242294.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JN691_000781"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JN691_000781"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAY7242295.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JN691_000782"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JN691_000782"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAY7242296.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="JN691_000783"
     CDS             complement(4598..5320)
                     /locus_tag="JN691_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7242297.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JN691_000784"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JN691_000784"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7242298.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="JN691_000785"
     CDS             6520..6738
                     /locus_tag="JN691_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAY7242299.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="JN691_000786"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="JN691_000786"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY7242300.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="JN691_000787"
     CDS             complement(7472..7672)
                     /locus_tag="JN691_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAY7242301.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="JN691_000788"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="JN691_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAY7242302.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKTQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="JN691_000789"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="JN691_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAY7242303.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /locus_tag="JN691_000790"
     CDS             10169..12082
                     /locus_tag="JN691_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7242304.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /locus_tag="JN691_000791"
     CDS             12082..13104
                     /locus_tag="JN691_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7242305.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /locus_tag="JN691_000792"
     CDS             13098..13316
                     /locus_tag="JN691_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAY7242306.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /locus_tag="JN691_000793"
     CDS             13370..14239
                     /locus_tag="JN691_000793"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAY7242307.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /locus_tag="JN691_000794"
     CDS             complement(14294..14698)
                     /locus_tag="JN691_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAY7242308.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="JN691_000795"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="JN691_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAY7242309.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /locus_tag="JN691_000796"
     CDS             15683..17773
                     /locus_tag="JN691_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242310.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="JN691_000797"
     CDS             complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="JN691_000797"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAY7242311.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGWARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="JN691_000798"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="JN691_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAY7242312.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19741..20343)
                     /locus_tag="JN691_000799"
     CDS             complement(19741..20343)
                     /locus_tag="JN691_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAY7242313.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /locus_tag="JN691_000800"
     CDS             complement(20333..20500)
                     /locus_tag="JN691_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAY7242314.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="JN691_000801"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="JN691_000801"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAY7242315.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="JN691_000802"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="JN691_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAY7242316.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNNAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /locus_tag="JN691_000803"
     CDS             22891..25434
                     /locus_tag="JN691_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAY7242317.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="JN691_000804"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="JN691_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAY7242318.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25883..26689
                     /gene="nirC"
                     /locus_tag="JN691_000805"
     CDS             25883..26689
                     /gene="nirC"
                     /locus_tag="JN691_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAY7242319.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26708..28081
                     /gene="cobA"
                     /locus_tag="JN691_000806"
     CDS             26708..28081
                     /gene="cobA"
                     /locus_tag="JN691_000806"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY7242320.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLQEAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28328..28495
                     /locus_tag="JN691_000807"
     CDS             28328..28495
                     /locus_tag="JN691_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAY7242321.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28790..30022
                     /locus_tag="JN691_000808"
                     /pseudo
     CDS             28790..30022
                     /locus_tag="JN691_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            30043..31065
                     /gene="frlB"
                     /locus_tag="JN691_000809"
     CDS             30043..31065
                     /gene="frlB"
                     /locus_tag="JN691_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HAY7242322.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31115..31945
                     /gene="frlC"
                     /locus_tag="JN691_000810"
     CDS             31115..31945
                     /gene="frlC"
                     /locus_tag="JN691_000810"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HAY7242323.1"
                     /translation="MKTGMFTCGQQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31942..32727
                     /gene="frlD"
                     /locus_tag="JN691_000811"
     CDS             31942..32727
                     /gene="frlD"
                     /locus_tag="JN691_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAY7242324.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32827..33558
                     /gene="phoB"
                     /locus_tag="JN691_000812"
     CDS             32827..33558
                     /gene="phoB"
                     /locus_tag="JN691_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HAY7242325.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33718..34803)
                     /locus_tag="JN691_000813"
     CDS             complement(33718..34803)
                     /locus_tag="JN691_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY7242326.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34815..36119)
                     /locus_tag="JN691_000814"
     CDS             complement(34815..36119)
                     /locus_tag="JN691_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242327.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36131..36484)
                     /locus_tag="JN691_000815"
     CDS             complement(36131..36484)
                     /locus_tag="JN691_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HAY7242328.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36495..37373)
                     /locus_tag="JN691_000816"
     CDS             complement(36495..37373)
                     /locus_tag="JN691_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAY7242329.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37370..38596)
                     /locus_tag="JN691_000817"
     CDS             complement(37370..38596)
                     /locus_tag="JN691_000817"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY7242330.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38597..39760)
                     /locus_tag="JN691_000818"
     CDS             complement(38597..39760)
                     /locus_tag="JN691_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HAY7242331.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRGQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39844..40206)
                     /locus_tag="JN691_000819"
     CDS             complement(39844..40206)
                     /locus_tag="JN691_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY7242332.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40223..41128)
                     /locus_tag="JN691_000820"
     CDS             complement(40223..41128)
                     /locus_tag="JN691_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242333.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41398..42402)
                     /gene="trpS"
                     /locus_tag="JN691_000821"
     CDS             complement(41398..42402)
                     /gene="trpS"
                     /locus_tag="JN691_000821"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAY7242334.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42395..43153)
                     /gene="gph"
                     /locus_tag="JN691_000822"
     CDS             complement(42395..43153)
                     /gene="gph"
                     /locus_tag="JN691_000822"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAY7242335.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43146..43823)
                     /gene="rpe"
                     /locus_tag="JN691_000823"
     CDS             complement(43146..43823)
                     /gene="rpe"
                     /locus_tag="JN691_000823"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAY7242336.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43841..44677)
                     /gene="dam"
                     /locus_tag="JN691_000824"
     CDS             complement(43841..44677)
                     /gene="dam"
                     /locus_tag="JN691_000824"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY7242337.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44784..46070)
                     /gene="damX"
                     /locus_tag="JN691_000825"
     CDS             complement(44784..46070)
                     /gene="damX"
                     /locus_tag="JN691_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAY7242338.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTELAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQVDLK"
     gene            complement(46162..47250)
                     /gene="aroB"
                     /locus_tag="JN691_000826"
     CDS             complement(46162..47250)
                     /gene="aroB"
                     /locus_tag="JN691_000826"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAY7242339.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPRKMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47307..47828)
                     /gene="aroK"
                     /locus_tag="JN691_000827"
     CDS             complement(47307..47828)
                     /gene="aroK"
                     /locus_tag="JN691_000827"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAY7242340.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48229..>48347)
                     /locus_tag="JN691_000828"
     CDS             complement(48229..>48347)
                     /locus_tag="JN691_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242341.1"
                     /translation="VPLLGDIPWFGQLFRHDGKEDERRELVVFITPRLVSSE"
BASE COUNT        11644 a        13150 c        12088 g        11465 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag tgccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccaaga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctag tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcaatgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaagccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggctcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctgggtttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaatg
     8761 taccgagcgc catcgacagc cccagcgcat ccataaacaa tgcggaaccc aacaccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaag cggaataccg
     8881 gacgcagtaa atagcgccca ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg accccgccaa caacggcact aacgccagtg
     9001 ctgggatcac tgccagatcc tgaaacagca gaaccgaaaa cccgagctgg ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa ggccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatccccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccaac cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctttg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcagcacat cgctcatcgg
     9661 gtagcgattc agcgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgtt
     9721 acgccagtac tttcccgcca gttggtttcc tcccggccca ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgcca gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tgcacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat agcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtacgaaag
    10921 ccagcaggaa cgcgtggcac atctgcaaag ttatatcgat cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctggaa cgtatggagc tgattgcccc
    11041 cgcacacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc tcgactcgat
    11161 taaattgaat ctggtccccg gctcgcgcat tggtctgtta ggccgcaacg gcgcaggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttcctggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaatttagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa acataaaccg
    12301 cgtttagtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctaatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccttttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctcccctcgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aatagtgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccctgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtagt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcggtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccttg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtacctgg
    14161 cggcaaaatg gggctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tttgctatta tgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aagtcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cggcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctccctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacactt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggtaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gctggggcca ttgcacgcaa aattgcttcc
    16021 tgcatcgctg ctcgccgcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accattgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgatcctg aaaaagcgct
    16261 gccaccgctg ctggtgcgcc agcaaaaagc ggtcgattta attactcagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaacaatac cgattacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttgcagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaaactggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttac ttccggcact
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgt gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtagcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg caacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actgttgtgg ctgaacggtg agcagtacat ccttccacgt cttatcgata ccattattgg
    17161 ttgtttgatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgatg ctttagaagc ctatcaggaa gcgattcgct tgattctgag
    17281 cgaggacccg caaccaacgc cactggcctg gcagagaatg cgggtaaatc aggcgcataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcattcaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggccatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatacaggc taaaccatcg gataaggcat tcacgccgca tccgacatat
    17821 tttgcctgat gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgctaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccaa cctctgcacc cgcatacagg aaatcacgcg
    18001 cccaaccttt gtagtgtggt ttcagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaagtcag aatatccggc gtcacaccgt agtacctgta agcaaacaaa tcgccagtcc
    18361 gccccatccc gcactgcact tcatcaaaca ccaataatgc ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgca tggagatcgt
    18541 taaagggaac gtggatgatg tctgccggtt tcggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga ataaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcactg aatggcaatc
    19141 ggaaatcaga gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ctatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttgccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gtgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tggtacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggcggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac atcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcgacg gcgatgttca ttctcggggt ggtcagcggc
    21781 attactatgt cgattggtac attcctgata acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttactga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcact gggcaaacgt
    22021 gcaccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac aacgcgggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23221 tcctacccgt ggatcccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt ggggggattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggtggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctatgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaacgct
    24061 atcgagctgc cggaaaaccc ggattctctg attctgccag cgcactcggg tagcggtaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 actaaaggtg atctgattgc tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa
    24241 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24841 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca
    25561 acattgatcc gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25861 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaatgc
    25921 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25981 ggcgggcgca tatgtgggtc ttgggatcat cctgattttc acgctcggta atttgctcga
    26041 cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26101 tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc
    26161 gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26221 ggtcggttcc gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt
    26281 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26341 gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gcaatctgga tggcgctgcg
    26401 cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc
    26461 cggctacgag cactctatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26521 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26581 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26641 tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg
    26701 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26761 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26821 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26881 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26941 agcgacggat gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    27001 cttctgtaac gtagtcgatg cgccgaaagc cgccagcttt attatgccgt cgattattga
    27061 ccgctcaccg ctaatggtag cggtctcctc tggcggcacc tctccggttc tggcgcgcct
    27121 gttgcgcgaa aaactcgaat cactgctgcc gttgcatctg ggccaggtgg cgaaatatgc
    27181 cgggcaatta cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc gtcgccgttt
    27241 ctgggagaaa ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa
    27301 agccattact gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27361 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag gactgcaaca
    27421 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27481 ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc
    27541 gcaggaagag attaaccaga tcctgctgca ggaagcgcaa aaaggcaaac gcgtggtgcg
    27601 gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27661 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc
    27721 ctattcgggt attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg
    27781 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27841 ggtgttctat atggggttga atcaggccgc gactattcag caaaagctga ttgaacacgg
    27901 tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt
    27961 gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct
    28021 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaatcatta
    28081 atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa
    28141 taaaacataa ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga
    28201 cttacaaaat taagtcattc gctcactgac gccaattaaa tattcctgta tcgttcttaa
    28261 cgagcagtct gcttatattc atataatcaa tgaatattaa ttaatatata atacataggg
    28321 aatgtaaatg aacaaattta ttaaagttgc actggtaggt gcagtactgg ctacgttaac
    28381 tgcatgtact ggtcatattg aaaaccgtga taagaactgc tcttacgact acctgctgca
    28441 cccggcaatt tctatttcta aaatcattgg cggttgcggt cctactgcac agtaagtctc
    28501 tgacagaaat cggctaacac cggtttaatt gcccgacgcc ctctggattt tccgagggcg
    28561 tatttgtttc tatgtccttc ttgcccccgc ccgtaaaaat attttcactt taaattcaat
    28621 ttgataacta catccattag ccacagcgcg atcctgtgcg aaattttgtg atcttcctcc
    28681 acattacata acatcatatg ttgttatatg catcatgcat tgtcatgtta ccttttaaat
    28741 gactgcaaac tctcccctac aacgtattgg acaagaaaaa ggtatcgcta tgggaagcca
    28801 ggaactccaa cgcaagctcg gattttgggc cgttcttgca atcgccgtcg ggacaaccgt
    28861 cggctccggt atttttgtat ctgtgggtga agtggcaaaa gcagcgggca cgccgtggct
    28921 tacggtgctc gcgtttgtca ttggcgggtt aattgtgatc ccgcaaatgt gcgtctatgc
    28981 ggaactatcc accgcttatc cggaaaatgg cgcagattat gtttatctga aaaatgccgg
    29041 aagccgaccg ctggctttcc tctccggctg ggccagcttc tgggccaacg atgcgccgtc
    29101 attgtcgatt atggcgctgg cgattgtcag caatcttggc tttttaacgc ctatcgatcc
    29161 gttgctcggt aaatttatcg ccgccggatt aattatcgcc tttatgttgc tacacctgcg
    29221 ctccgttgaa ggcggcgcag cgtttcagac gctaattacc atcgccaaaa ttatcccgtt
    29281 cactatcgtc attggccttg ggatcttctg gtttaaagcg gagaattttg ccgcccttac
    29341 caccactgcg attggcgcaa cgggcagctt tatggcgctg attggttcct gtctgctggt
    29401 tctggtgctc tacaccctgc tggcgctggt gatttccggc ctgatgccct tcgacaaact
    29461 cgccaattct gaaacgccga tttccgacgc cctgacctag atccccgcac tcggcagcac
    29521 cgctgggatc tttgttgcca tcacggcgat gatcgtcatt cttggttcgc tttccagctg
    29581 cgtgatgtac cagccgcggc tggaatacgc gatggcgaaa gacaacctgt tctttaaatg
    29641 cttcggccat gtgcatccga aatacaacac gccggatgtc tccatcatcc tgcaaggggc
    29701 gctggggatc ttcttcatct tcgtttccga tctcaccagc ctgctgggtt atttcaccct
    29761 ggtgatgtgt ttcaaaaata ccctcacctt cggctccatc atctggtgtc gtaaacgcga
    29821 cgattacaaa ccgctgtggc gtactccggc tttcgggctg atgaccaccc tcgccattgc
    29881 gtcaagcctc attctggtcg cctcaacctt tgtctgggca ccgattcccg gccttatctg
    29941 cgccgtcatc gttattgcta ctggtctgcc tgcttacgcc ttctgggcga agcgtagccg
    30001 ccagctcaac gctttgtcgt aatgttatct gaagaaaaaa aaatgttgga tattgataaa
    30061 agcaccgtgg actttctggt caccgaaaat atggttcagg aagtggaaaa agttctcagc
    30121 catgacgttc cgctggtgca cgccatcgtg gaagagatgg tgaagcgcga cattgatcgt
    30181 atttatttcg ttgcctgcgg ctcgccactc aacgcggcgc aaacggcgaa acatctggcg
    30241 gatcgctttt ccgatcttca ggtctacgcc atttccggct gggagttctg cgataacacc
    30301 ccgtatcgcc tcgacgatcg ttgcgcggtg attggcgttt ctgactacgg taaaacggaa
    30361 gaggtaatca aagcgctgga gctgggccgg gcctgcggcg cactcactgc ggcgttcacc
    30421 aaacgcgcgg acagcccgat tacctcggcg gcggaattta gcattgatta tcaggccgac
    30481 tgtatctggg aaattcacct gctgctctgc tacagcgtgg tgctggagat gatcacccgc
    30541 ctcgcgccga acgcggaaat cggcaagatc aaaaacgatc tcaagcagtt gccgaatgcg
    30601 ctcggtcatc tggtacgcac ctgggaagaa aaaggccgcc agcttggtga actggccagc
    30661 cagtggccga tgatttatac cgttgctgcg ggtccgctgc gtccgctggg ttacaaagaa
    30721 ggcattgtaa cgctgatgga atttacctgg acgcacggct gcgtgattga gagcggagag
    30781 ttccgccatg gcccgctgga gattgtcgaa ccgggcgttc cgttcctgtt cctgctcggc
    30841 aatgatgaaa gtcgccacac caccgaacgc gccattaact ttgttaaaca gcgtactgac
    30901 aacgtgatcg tcatcgatta cgccgaaatt tcgcaagggc tgcacccgtg gctggcaccg
    30961 ttcctgatgt tcgtgccaat ggagtggctc tgctactacc tgtctattta caaagatcac
    31021 aacccggatg aacgccgcta ttacggtggt ctggtggaat attaatccct ctcccggccc
    31081 ggcaacgggc cggatttacg caaggagtta cccgatgaaa acaggtatgt ttacctgcgg
    31141 ccagcagcgg ctgcctattg aacacgcatt tcgtgatgca agcgagctgg gttacgacgg
    31201 catcgaaatt tggggcggtc gcccgcacgc gttcgcgccg gacttaaaag cgggcggcat
    31261 caaacaaatc aaggcgctgg cgcagacgta tcagatgccg attatcggtt acacgccaga
    31321 aaccaacggc tatccgtata acatgatgct gggcgatgaa catatgcgtc gtgaaagcct
    31381 ggacatgatc aagctggcga tagatatggc aaaagagatg aacgcgggtt atacgctgat
    31441 ttctgcggcc cacgcgggct atctcacgcc gcctaacgtt atctgggggc ggctggcaga
    31501 gaacttaagc gaactgtgtg agtacgcgga aaacatcggc atggatctga tcctcgaacc
    31561 gttaacgccg tatgaatcga acgtcgtgtg taatgctaat gatgtgcttc atgcgctggc
    31621 gctggtgcct tcgccgcgcc tgttcagcat ggtcgacatt tgcgcgccgt atgtccaggc
    31681 ggaaccggtg atgagttatt tcgacaaact gggcgataaa ttacgtcatc tgcatattgt
    31741 cgacagcgac ggggccagcg acacgcatta cattcctgga gaaggcaaaa tgccgctgcg
    31801 ggaactgatg cgcgatatta ttgatcgggg ctatgagggt tactgtacgg tggagctggt
    31861 gacgatgtat atgaacgagc ccagactcta tgcccgccag gcgctggaac gctttcgcgc
    31921 gctgctgccg gaggatgaga gatgaaaacc ctggcgacaa tcggcgataa ctgcgtcgat
    31981 atctacccgc aactgaataa agcgttttct ggcggtaatg cggtcaatgt ggcggtgtac
    32041 tgcactcgct acggcataca gccgggatgc attacctggg tgggtgacga tgactacggc
    32101 acaaagctga agcaggatct cgcccgcatg ggcgtcgata tcagccatgt ccatacgaaa
    32161 cacggcgtta ccgcacaaac tcaggtggaa ctgcacgaca atgatcgcgt ttttggcgac
    32221 tacaccgaag gcgtgatggc cgactttgcc ctgagtgaag aggattacgc ctggctggcg
    32281 cagtatgaca ttgtgcacgc ggcaatctgg ggacatgcgg aagacgcatt cccacagctg
    32341 cacgctgcgg gcaaacttac cgctttcgac ttctccgaca agtgggacag cccgctctgg
    32401 cagacactgg tgccgcatct cgattttgcc tttgcctccg caccgcaaga agacgaagcg
    32461 ctgcgtctga agatgaaagc gattgttgcc cgtggcgcag gaacagtgat tgtcacgctg
    32521 ggtgaaaacg gcagcattgc ctgggatggc gcgcagttct ggcgtcaggc tcctgaaccg
    32581 gtgacggtta tcgacaccat gggtgccgga gattcgttca ttgccggatt cctttgcggc
    32641 tggtctgcgg ggatgacatt accgcaggcg atggcgcagg gaacggcgtg cgcggcgaaa
    32701 accattcagt accacggtgc ctggtaggta taacgttggc gtgagcatct tcacgccaac
    32761 gtgctgttac ttgccggaaa acgaccctat aatccgagta attcattctt tatttcaggg
    32821 ccgattatgt cagctacgga ccgctactct catcaactcc tctacgctac cgtccgccag
    32881 cggctgctgg atgatatcgc gcagggggtt taccaggccg ggcaacagat ccctaccgaa
    32941 aacgagcttt gtacacaata taacgtcagc cgcattacca ttcgcaaagc catcagcgac
    33001 ttagtggcag acggcgtact gatccgctgg cagggaaaag gcacctttgt acaaagccag
    33061 aaagttgaaa acgccctgct tactgtcagt ggttttaccg attttggcgt ctcacaaggc
    33121 aaggcgacga aagagaaagt gatcgaacag gaacgggtca gcgccgcgcc gttttgcgaa
    33181 aagctgaaca tccccggaaa cagcgaagtg ttccatctct gccgggtgat gtatctcgat
    33241 aaagagccgc tgtttattga tagttcatgg atcccgctgt cgcgttatcc tgactttgat
    33301 gagatttacg tcgaaggaag ctccacctat cagttatttc aggagcgttt tgacacgcga
    33361 gtggtcagcg acaaaaagac catcgatatc tttgccgcca cccgcccgca ggcaaaatgg
    33421 ctgaaatgcg aactgggcga accgttgttt cgcatcagca aaatcgcctt tgatcagaat
    33481 gacaaaccgg tgcacgtctc cgaactcttc tgccgcgcca atcgcatcac cttaacgatt
    33541 gataataaaa gacattaacc gtaggccgga taagatgcgc cagcatcgca tccggcgatg
    33601 ctggcgcact gaattttaca tcccgcacgt tcccctcacc ctaaccctct ccccaaaggg
    33661 gcgaggggac cgttcagtgc ggaatttagc ttgattgccg aacggtgcta tccagcatta
    33721 aatactcgca atactctcac gcaaaatccg cagcaccgtc tcttcaccgc tgcgattcgg
    33781 gttaatgcga atcgcacaat gttcgagttg tggattcgcc tggcgaaacg ttcctgaaag
    33841 tcgatagaag agcggcggga tttcatattt cgactctgca ccgaccgggt aaggcaacgc
    33901 gccacgcttt tgcgcttctt ccagcaccct ggcggcaatc agctgatgaa actcgacaat
    33961 caacaccttc gactgcgcat tggcaatgac ggcgcttttc acttccgcca ccgcgccgcc
    34021 gttaagcaaa gccagcaacc gttcggatac tcccgcctgc accgcatgca tcactggcgc
    34081 gaacaccaga ccacgcaaga cttccagcgc ctgtgagccc tggatctggc taccgccgga
    34141 gtaaagcgtg gcgcgaatcc gactaataac atcggcatcg ccaaccactg caccaacgcc
    34201 ctccggccca aacagcttga agcaggagaa tgtcgagaca ttcgcgccgc attcacagcc
    34261 gattcgcgct accttcatca ccgcatagtt atcatcggtt aacgctggaa cacctgccgc
    34321 gcgcaacgtt gccagcacat ctgccagcac atagctgtcc tgcggctgct ggcgcgtatg
    34381 ctgcaccagc gcggcatcag gttgttgctc gtcgacgacc tgcttcagtg ccgacaggtc
    34441 attgaaatca gcagtaataa gcgtcagccc catctgctca ataataaccc gtgtcgtcgg
    34501 gtaaacaggc gcgtcatgca ccagaagacg ctgccccggt ttgagcaaag ccgccagccc
    34561 ggcgcgaatc gcgccagtcc ccgcgccctg caccagcgcc gcggcctgtg cgtgaaatgc
    34621 atcagccagc acctgctcca cccgttgggt aatacgcggt tgattcagac ctggagttaa
    34681 ccctaaatca ccgccagcaa gaaactcgct gcctggaaaa tggcggcaaa tagtgtccac
    34741 cagcgcaaac tgcttttgct gcgcttcagc aagagtcagg ctttgcaaag gaaacgtctt
    34801 cataggtgcc ttccttaagc cggaacgaac aggccaagcc agtaaagaac attcagcaga
    34861 ataccggtga tcattactgc cacgaccggt gccgccattt tctgtaccgg acggcccagg
    34921 gattcgttga ggaagtaaat ggcaaccgcg atagagaatc cggtataacc cgccatctta
    34981 attgctgcga aaatcgaacc gaccagcagc gccacttcca tcagcatatt catggcgtta
    35041 cggatgttat ccgacgcatt acgcaccgac gggtagcgtc ccagccattt gccgatcgaa
    35101 cgaagcagca agacttccgc cgaaataacc actgcgccta ataccgctgc aaccatcgga
    35161 ttcggcgaga gatagcccac cgcataaaca aaggtaaagc ccgcaactgc atacacaccc
    35221 gttgctaagg cggtggtggc aatcaacggc acaaacccca gtccgcgcat aaattctgcc
    35281 agagccgcct gattaatcag cgtttgcgat tgttccggcg tcacgcctgc ggaatacgct
    35341 ttctccagtg tgaagatcga aacttcgcta ccagcaaaaa tcttcatact ggcaacggcg
    35401 gcaatcaatg ctcccacgat ggcgatataa ggtaagtttt tgataatccg cgacgtgcgc
    35461 tcttcaaaca ccgaaagccc gctggcatcg atgtcatttt catcacgatg acgcaggtcg
    35521 tgagttatcg cgatccccag cagcatcacc atgccaataa agatttcgat ggattcaggg
    35581 ttaagatgtg ggaaatagcg cacgactacc acacgggtca tcagaacaac cacggcggcg
    35641 accagacttt gcttccagcc aaactggtag aaaatcgcca ccagcgggaa cagtgcaaaa
    35701 gcagaaacca ccggcgagct taattccccg aggctaccta atacatccac cggcagcgcg
    35761 gtcagcagct ggttaactgg caacaggcaa gtaaggatca acacgcccca gatagcgcca
    35821 agaccaaacg ccatcaggct gtttatcgcc agtacgccga ggatatcggt aggaagaaag
    35881 agtaaccatg cgttgagcag cccggttttc agggtaaaag agatccccac cgaggccaca
    35941 aaaccaatgc tcagaccaaa agcgatactc cccgcctcgc gacggttcat atagccttca
    36001 atcagttgcg gcaggatcgg gtggatgccg tcatgaaaaa cagccgccga gcgatgcgcc
    36061 agaagcgatg tcatacccgt caggcacgcc accacgataa tctgaatata cagatccata
    36121 gtcgtgcctt ttatttcaga tggttaatca acatcggaat cgcgtgttcg acgtgctcaa
    36181 cggaaaggcc aaacgccact ttcccttcag cgatcatttt ggcgatatgc tcgtctttcg
    36241 ctttaatgcc tggtttggca atggtgcaac ttttgttata gccaatcacc gcgatggcaa
    36301 tcgacaatgc cgcgcccgca ccggtattac acgcgccaat gtaataatct agttgcccgg
    36361 atttcacctt cattgccgct tccatgtcgt tgtgaatgaa aacttcaaaa cagccaggag
    36421 ccgtcgcttc aatggttttt ttaatctgct cacgctgtaa gcctgcaacg ccaatctttt
    36481 tcatgagtct gtccttattg gaaaaattga gagggatttt cacgtaacat cacatccaca
    36541 tcggcctgac tgaatcctga ctggcgcaat tgcggaataa aggtggtcaa taaatagtca
    36601 tagccataac caccattggc ttttaaatgg gagcggcgcg taatatccat cgacagcatg
    36661 acgcggttca gcaacccacg gtcacgtagc gcgtggagca tcgcaatgcg cttttcgtcc
    36721 gggtagtaac tgttcttacc aatggtgtcg aactgcacgt acgcgccgag atcgatcatc
    36781 ttcaaaatgt tgtcgaggtt gtctttcaga tcgcagtgac caacggtgac gcgcgaaaga
    36841 tcaactccgt gggcttgcaa cagagccagt tgttccagcc ccatggtgct gaacgacgta
    36901 tgcgtggaga tcgggcgtcc ggtctggtta tgcgccagcg cagcggcaat aaataccttc
    36961 tcttccagcg gcgtaatctt tccttcgctg gagccgatct ccgcgatgat cccggctttc
    37021 agctccgtgc catcgatacc ctgttcaatt tcatcgacca tctcctgcgc cagttcctgc
    37081 acgctgcggg tcaccacatg ttccgggaaa aacgcatcct ggtaataacc agtacaggcc
    37141 accacgttga tcccggtttc gcgcattaca tcgagcataa attgcgcatt gcgccccatg
    37201 taacggttgg tcatctcaat cacattacgt acaccccggg tcatcaggtc gttcatctcc
    37261 tggcaaatga acgcatactg atcaaggcgg cagtccacgt tgtttttaaa gccggagaga
    37321 tcaatatgca gatgctcatg ggccagggtg taacccgtcg gatcaaaact catagggtgt
    37381 ctcctgcaaa ccggagggag gaaagaaaag agcgaccatt ttgtggcggt ggcgcacgga
    37441 aaaattcaca cacggtagct cccacatcag aaagcgtggt gcgcacaccg agctgcgtgg
    37501 cgaccagccc ttgctgataa accagcactg gcaccacttc acgggtatgg tggctgtgac
    37561 caatggtcgg atcgttaccg tgatccgcca tcacgaccag gcaatcatct ggctgcatcg
    37621 cctcaacaag ccgggcaagg ttacggtcaa cgacctgcaa acgttcggca taacgtgcga
    37681 cgtcttctgc atgaccagcg aggtcggttt cctggatatt gatgcaaata aacgccgtcg
    37741 gatgggtgtt aaattcgtcg agggtgatat ccataatccg ctggctatcc accaggtttt
    37801 gccagctcac accataagga ttgctgacga tatctgccac cttacccacc agcacggttg
    37861 gcacgcccgc ttcatacagt ttttgcggca cctgcacttt ttcatcaaca ccgtagccca
    37921 tatgcacgac ctggaagccg ttgtcataag cgccagaacg cggcgcatta ataccaataa
    37981 agcgcccttc tttgctttct gcggcatcga gaatacgctg actgttggtt aacaggccgc
    38041 caaatgtaat gacccgaccg acctgtacct ggtcacgcac gatacgacca attttgattg
    38101 cgtcgtcaaa agagatcaca gagagattgg cggtaatgtt atagacttgg cctaaatccg
    38161 cctcgagatt atcgccaatc gcaaccgcct gattgaccca cagaaattgc agatcatcgc
    38221 cacggcgctc cacctgccag ccagcggaaa ctaatgcctg ctcaacacgg tcaatcacat
    38281 cgcgaaaagg catccgcagc ggcggtaacg ggcgcgtgcc taaaatttcc tgatgcccca
    38341 taaaggtatc gccaccttca tgttgcagct ctgccacgcc ccaggttgcg gaatctgacg
    38401 gctgcatatc gcctggcgca taacccaatg tgttgattag ccccagcttc tccagcgttg
    38461 gtagctgcaa atgcggcaac tggctcagga tgtgaccaca ggtattcgcc ccggcatctt
    38521 gcgggcgcac cagcgtgaca tctttcattg cccctacgcc aaagctatca atcactaaca
    38581 ccacaaatcg cgccatttat gcctccagag ggttgccgag gctgtcataa cgaccaacta
    38641 tttctggttc gccgcgctga ataccggaca ccagcaccac atcgctacgg gtgacaaaaa
    38701 tctgcgtgcg aaaacagagc accactgcac tgctcaccgg atactcgcct gccagcggca
    38761 gggtgtagtc gatactgctg tcatccacag ttttgagatt ggtttcagta atcttttgat
    38821 tttctggcgt aaacaccagc gcatgttgcg catgaccacg acggtagtaa ccgccgccgt
    38881 agcagtagct gtcgccacgg aaatgatggg agatttcact taaccagagc atcgcgatac
    38941 gttcaggctg atcgccctgc tggtttgccg gaatagtacc ggtcagcgca tgaccgggtt
    39001 cggcatgagt cgcaccgtat tccgccagca atggcagcga agtgcagctg gtcgctgaag
    39061 gtacgttcag ttgctcaatc gcaataccag atttcgccag ttgatcccgt gcttgtacca
    39121 gcgtgtgaag attcggtgtt ggcaaaactt tcccggcagc ctcatcccaa agcaggcaag
    39181 ggaaatgggt tagtccggct aaatgcagcc ccggcagttt cttaatttca gcaatcacct
    39241 cgtgcagcga atgctgtaca aaaccgctct cctgccccgg ataaagaaaa tcatcgtcgc
    39301 tataaacttt gagcagcaca ggctggcccc gtccggcctt aaccgctgcc gcagaaactt
    39361 cccgcgcttt gtcgagagta aacacggtga tgatgtcggt gccctgttca acggcgtcag
    39421 caacctgatg acaagggatt tgtaccagat gcccctgatg cgccactggc aaaccagcgc
    39481 ggcgcatgac tcgcgcctct ttgtaatcca ccgccacaat gccgctgtag cctaatgcca
    39541 gcaatttttc cgccagccac ggattacgac caaattgctt ggtcatcaga tacagttcaa
    39601 tgccgtaaag ccgcgccgtc tcaatgagcc gcttaccgtt ttccagtatc tgatccacgt
    39661 cgatcaccca gctgtccggg gcgattttgc cctgctgcca caaacttagt gcggcggaaa
    39721 tcagcgccgg gttctggcgt ttcaatgctt ctacaaacat cttgttgcct cgtttctgtt
    39781 ccttgaatta aatattcatt tttttgaata tttaggttgt gcgacgtgga tttgtcggga
    39841 gcttcaaacc tcttcgttag cagccatcca taatccataa agattggcca gcaaatagcc
    39901 ttcttcacac gggttaactt ccagagcgaa ttccttcaac aacacctgat gcaattgcac
    39961 aacggcttgc cagtggctgg attgcgccag ttccgccagc aactcgttat ccagcggctc
    40021 tatttcttca ccacggcgac tgcgcatcag tgcactcgcc atatgtgtca tcgccatcgt
    40081 tccttgctca ctgcgcaccg gcagatggca ctctttttcc agtacgttga cgacctgcat
    40141 catgcctttg cagacgtcct tatcaataac gcctgcctcg caaagcaggt tgagtctggt
    40201 ttccatatca gatgcctttc gtttaataac tgggttcctg ttccccactc accacccgtt
    40261 gttgatgggc aagcagggcg tgtttatcta caacggcacg cagcaattgt tgcattgggt
    40321 aatcatcgac tcgcgtcagg accacggctt cggtggcctg taaaaatcgc ggatcgtctg
    40381 ttagcggcgt cgcctctaat cccagcatgg tcagttcgtt ttccgccacc acgttccaga
    40441 tcaccgcatc gacatcgcct ttaacaatgc gttgtaaact ctcgtgataa gagagatcga
    40501 ctcgttccac atcactatcg ccaaaaaaga catcggtcat gattttctga tccgccgaac
    40561 gactatcgag acctactcgc ttaacatttc cagactcacc tttgcggcat atcagttgat
    40621 gttcaccaac atacgtatgc gggccaagct cgagggcgat gcacaaattg ttttggctga
    40681 gataactttc cgccgccagg cgggaaacta ctgccatgtc atagacaccg ttaagtaaac
    40741 actccacgcg aatatccgcg ccgcgcatat gcgcatagta aaaaggaatg ccatcaaact
    40801 gggctttcaa tccgctcgct aaaccttcgt acaagcgggt atagggcaaa ggcatcgcac
    40861 ataccacgtt gttgatatcc acatgactca gcaatgcttt gttatccatc tcgacaagat
    40921 aactgccatt gcgcccacgg cgttcaatcc gtatcgctcc gcttgattcc agcgttttca
    40981 acgcggcctg cgtcaggcca accgatgaac ggcattcagt tgccagctca tctatggttt
    41041 tcagtcgatt accgcatttt tcacccaaca aataacgggc cagtgtcgtg atgacgacgc
    41101 cttctttttt gataaacgtt cgacgcataa tatattttca ataaaatgaa tatttatatc
    41161 ttcattaaaa tgaagataag tggcaacgac gagatgtgga aacggcgagg cgcttcacat
    41221 tttttcgggt tgttgcgaaa agaggataaa aaaaccgggt ttccccggtt tcagagtgat
    41281 gacgaagtga tttattcgac aatgcgagca aagggtaatt cccactggcg cttgcctgct
    41341 cccctcaccc taaccctctc cccaaagggg cgaggggacc gtccgagcgt tttgatatta
    41401 cggcttcgcc acaaaaccaa tcgcttcgta caccgctttc agcgtacggg aagcgtgtac
    41461 actggctttt tctgcgccgt ctttcatcac ctgttgcagg aaggcttcat cgttgcggaa
    41521 acggtgatag cgttcctgca attcagtcag cataccggaa acggcatcag ccacttcacc
    41581 tttcagatga ccatacatct tgccttcgaa ctgtttttcc agttctggga tgctctggcc
    41641 cgttaccgca gaaaggatat ccagcaggtt ggaaacgccc gctttgttct gcacatcgta
    41701 gcgaactacc ggcggctcgt cggagtcagt gaccgcgcgt ttgattttct tcactaccga
    41761 tttcggatct tccagcaggc cgataacgtt attgcggtta tcgtcagact tggacatctt
    41821 cttggtcggc tccagcagcg acattacgcg cgcgccagat ttcggaataa acggctccgg
    41881 caccttaaag atctcgccat acagcgcgtt gaaacgctgg gcgatatcgc gactcagttc
    41941 caggtgctgt ttctggtctt cacccaccgg taccagatta gtttgataca gcaggatgtc
    42001 cgcagccatc agcaccggat agtcaaacag accagcgttg atgttctcgg cataacgcgc
    42061 agatttatct ttaaactgcg tcatgcgact cagttcgccg aagtaggtat agcagttcag
    42121 tgcccagcct aactgcgcat gttctggtac gtgggactga acaaaaatgg tgcttttctc
    42181 aggatcgata ccacaagcca gatacaaggc cagcgtatcc aacgtcgctt tacgcagctt
    42241 ctgtgcatcc tggcgcacgg tgatcgcgtg ttggtcaacg atacagtaaa tgcaatggta
    42301 gtcatcctgc atgtttaccc actgacgcag cgcacccatg tagttaccaa tggtcaattc
    42361 acctgagggc tgtgcgccac taaaaacgat gggcttagtc atttttcgat tcctgatttt
    42421 cgctatgcgg aagccctaat gcgggcagaa ggtcatttat agactggtaa attacatcag
    42481 gctggctgag atcgatagcc tcgccgtagt tatatccgta ggttaagcca actgatgggc
    42541 aacctgccgc ttttgccgcc tgaatatcat tgcgtgagtc gccgacaaac agcatctgtt
    42601 gtggggcaat tcccatccgc tcagccacca gtaacagcgg gtccggatgc ggttttttgt
    42661 tttgcacatc atcgccgccg ataaccacgc taaagtattt ggcaatatct aaggcttcga
    42721 gcagcggcgc gacgaacggc gtcggtttgt tggtgaccag gcctagcggc aggcctttag
    42781 cctgcaacgc gcccaacgta tcggcaacgt gcgggaacaa aaacgtcccc tcttcggcaa
    42841 cctcgccata gtagcgatcg aacagtttac gcagaatacg tacctgttct tctgccggaa
    42901 tgtcgtcatc aacgggcggt ttccccattg ttttacgcag agtcgcacgt tcctgacgcg
    42961 cccaggtcaa tgcgcgctcc atcagaacat ctgcgccgtt accaatccag gtaataacgc
    43021 gttcttcacc tgcgacgggc aactccagcg catacagcgc catatctacc gcagcagcaa
    43081 gaccaggagc actgtcgacc agcgtaccat caagatcaaa agcgacgccg cgaatatctt
    43141 caaacttatt catgacttac ctttgccagt tcactgcgca tttcatcaat gactttttta
    43201 tagtctggct ggtcgaagat tgccgaaccg gcgacgaaca tatccgcgcc cgccgcagcg
    43261 atttcgccaa tgttgttcac cttcacgcca ccgtccactt ccagacgaat gtcaaagcca
    43321 gactcgtcga tacggcgacg tacttcgcgc agtttatcca gtgtttgagg aatgaaagac
    43381 tgaccgccga aaccagggtt gacggacatc agcaggatca catccagctt atccatcacg
    43441 taatccagat agctcagagg tgtcgccggg ttaaatacca gacccgcttt acagccattt
    43501 tctttaatca gttgcagcgt gcggtcaaca tgctcggagg cttctggatg aaaggtaatg
    43561 atgctggcac cagcggcagc gaaatcaggc acaatgcgat cgacgggttt caccatcaga
    43621 tgtacgtcga taggggcggt aatgccgtag ttacgcaagg atttcagcac cattggccca
    43681 atcgtcagat tgggaacata gtggttatcc atgacgtcaa aatgcacgac atcagcgcca
    43741 gctgccaggg cttttgcggt atcttcaccc aggcgggcaa aatcagccga cagaattgag
    43801 ggggcaatca aatactgttt catccgcttc tccttgagaa ttattttttc gcgggtgaaa
    43861 cgactcctgg tttgtacaaa gccagcagtt cgtccacctt tttacgtgtg ccgccgttgc
    43921 tgcttatact gcgtcgaact ttgacgacat gcaattttgc gcgctgatac cactcacgcg
    43981 ttaacatcgt atcgtgattg gagatcagca ctggaatatg gcgctcaacc agaccttcgg
    44041 cgatctccgc cagatgcgct tgttgttcaa gcgtaaaact gtttgtgtga tacgccgtaa
    44101 agttggcggt cgcagacagc ggtgcataag gcggatcgca atagacgacg gatgcatcat
    44161 ctgcgcgcgc catgctatcg gcgtaagact cacaatagaa aaaggcattc tgcgcttttt
    44221 cagcgaagtg atacaactct gcttccggga aatagggttt tttgtagcgg ccaaacggca
    44281 cgttaaactc accgcgcaga ttgtaacgac acaggccgtt gtaaccgtag cggttcaaat
    44341 ataaaaacag taccgcccga cggaacggat cctggctttt gttgaactct tcgcggaact
    44401 gatagtaaac ctcggcgcaa tttgtttcgg gaacaaacag ctcgcgtgcg gcctgtacgt
    44461 actcatcagt acgcatcttc acaatgttat agagactgat caggtcgcta ttgatatcgg
    44521 caaggatata acgagaaaag tcggtgttga gaaacaccga cccggcacct acaaaaggct
    44581 caaccagaca ttcgcccttg ggcaaatgcc gtttaatatc atcaagcagg ggatacttgc
    44641 cccctgccca cttcaaaaaa gcgcgatttt tcttcatgct gactaactaa ttacaccttc
    44701 tccggctgtg gagaaagctc cgacagcatc cagcgcttca ggcagctccc gcaacgataa
    44761 accattgcgg gagataacct tgattacttc agatcgacct gtacctgacg cagcggtttc
    44821 gcccacgggt ttttggcctg aacatctgct ggcaatgtag ataccgcttt tttcgcctct
    44881 tctttcgaag cgtacacgcc agaaaccagg acataccacg gctgaccatt acgcgtcgtt
    44941 tcatagacaa cgtagttttt cagattctct ttcttcgccc aaccgttcag gttgtcgtag
    45001 ttagaggaac tgctcagctg cagagtgtaa tggctggacg gtgccgattt caacgaacca
    45061 acattacctg cggtcttccc tccagcggct ggtgttgccg tggtttgcgc cggggatgcc
    45121 gtctgtactg gagccgtagt cgccgtctct tttggtgctg gcgtagaagt cgcagccggt
    45181 gccttcgtgc tcgccacagg ggcagctagt tcagtacgct tcggcgtctg tgctaccggc
    45241 ttcggctccg ttttcacggt tgcttgcggt tttttcggtt caatcaccgc ctgctgacga
    45301 actgggcgcg tagtggacgg acgttcagcg gtttgcgttt tcgccgtgtc acgcgatgca
    45361 ttgccattgc gaacaggcgc aaccgttgcg ggttcagtcg gcaacgtgga attgaccgcc
    45421 acattgttca gctgttgctg attttgtggc tgggtcagcg cattgttcag gtcaccctgc
    45481 acttcaacac gttgttgacc atccgttgcc accggggttt gcccttgagt cggcgtagaa
    45541 gagatcggtg gcagagaaac atcctgctga gtattttccg cagacgtggt tcccggcgct
    45601 ggctgcacgc cattcgcctg atcggtcgca ttgccagcaa gatcaatact cttctcgcca
    45661 gacgcggttt gatcgctgga agtggtcgag ggggctttta gcgcagaacc gataccgatg
    45721 atcaacagca gtagaaccag aatgccgacg cccatcatca tatactgacg agaagcgggt
    45781 ttactggctg cttttttgcg cttacgcgga cgacgctcta cgcgctcttc atccacggtt
    45841 tcatcttcgg attcatcaat ttcttcttcg atttccggtt cctcattgcg ctctttttgc
    45901 gcacgagtcg gacggcgatc gtcagtgtca tcaagttcaa tatcatcaaa attgatctgc
    45961 ggttcgccac gttcagtacg ctcagaacgt tcagaagatt gacgagaacg accagtacga
    46021 cgatcgctgg gatcgggttt cagctcgtct tctggtttga attcatccat ttaacacccc
    46081 actaaaaggt taatgcttac cacgttgcaa ttaacctgaa gctaatagac cgcttgataa
    46141 gcggcctgac ctttcttgtt gttacgctga ttgacaatcg gcaatggcgt taagaacaag
    46201 ctcgtgcgaa acgccgctgc gaacttcact cttaccaatt gccaacggaa gaattaagcg
    46261 catctctccc gcaaggactt tcttgtcacg cagcatatgc ggtaaatacg cctgcgcgga
    46321 cattttgcgc ggcccattga ccggtaaccc agcccgcgtg agcagggtta taatacgctg
    46381 cgtttcggca gaactaaact gcccgagacg ttccgacgtc cgcgccgcca tcaccatacc
    46441 cgcagcgacc gcttcaccat gtaaccaatt gccatacccc atttcagctt caatggcatg
    46501 accaaaggtg tgtcccagat tcagtaaagc acgtaacccg gtttcgcgct cgtcggcggc
    46561 gacaacttct gccttcagtt cacaacaacg gcgaatacag tacgccattg ccggaccgtc
    46621 cagacgcaac aacgcatcca gattctcttc cagccagtta aaaaacgcac cgtcaagaat
    46681 aatgccgtat ttgatgactt ctgccagccc cgacgctaac tcacgcgggg gaagcgtttt
    46741 cagacagtcg agatccacca ccactgaagc aggctggtag aacgcgccaa tcatgttttt
    46801 accgagggga tggttgaccg cagttttgcc gccaacggag gaatcgacct gcgacagtaa
    46861 cgtcgtcggg acttgaatga aacgaacacc gcgctgataa ctcgccgccg cgaagccggt
    46921 cagatcgccc actacgccgc cgccaagcgc caccagcgta gtatcgcgac catgcggctt
    46981 ttgtaacaac gccgtaaaga cggtatcgag tacagccagg cttttatact gctcgccgtc
    47041 agggaggata acgctatcga cgttaacacc cgcctgttca agtacgccgc ggaccttatc
    47101 gagatacaga ggagccaggg tttcgttggt gaccaacatg acctgctcgc ccgatttcag
    47161 cggtaagaat gaagctggtt cattaaacaa accagatgcg atggtaattg ggtaactacg
    47221 ttccccgaga gtaacgacaa tcctctccat aacgcgacat ccaccttaat tactgtaccc
    47281 gcagacgagt gtatataaag ccagaattag ttgctttcca gcatgtgaat aatctggttt
    47341 gcaaccactt tagcgctttg atcatcagta cgaatggtca cgtcggcaat ctcttcatac
    47401 agcggattgc gttcattggc caacgcttcc agaacttcac gcggcggtgt ttcaacgtgc
    47461 agcaacgggc gttttttatc acgctgcgtg cgtgcaagtt gcttttcgat ggtcgtttca
    47521 agataaacga caacgccacg agcggaaaga cggttacgcg tttcacggga tttcacagag
    47581 ccgccgccag tagccagcac aataccctgt ttctcggtca actcattgat gaccttttct
    47641 tcgcgatcgc ggaagccttc ttcgccttct aaatcgaaaa cccagcccac atcagctccg
    47701 gttcgtttct caatctcttg atcggaatcg taaaattcca tattgagttg ttgagctaac
    47761 tggcgcccaa tagtgctttt tccggcaccc ataggcccaa ccagaaagat attgcgtttc
    47821 tctgccattt tttcggtact actaagacta ttcgttaatg ataaacccgc ttcgctcaga
    47881 gagcgccgca ggacatgaac tgaaacctca taagatattg cgagagtcag actgaaaatt
    47941 atctcaatac tccagcgggt ttggcaactg aataaatcac ccatccctct gcatatctgg
    48001 tcgctgcaag cgcgctgcct tgctaccacc gctctggcga taaatcaccg ggtaagatta
    48061 gcgtaaaaaa gacagcaaaa tgccgcctga atgataaatc atcatcatgg aacgcaggca
    48121 gcgaagagca ccaactctca aatcggtact ccttgtatgc taaatacctg cgcgcgtcaa
    48181 atagatgaaa cacgttcagc ataaaaacat taccgctttt acggctgttt actcactgga
    48241 aaccagtcgt ggcgtgataa acaccactaa ctcgcgtcgt tcatcttctt ttccgtcatg
    48301 acgaaataat tgcccgaacc agggaatgtc gccaagcaac ggtacgc
//
LOCUS       DADLSA010000014        47299 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.014, whole genome shotgun sequence.
ACCESSION   DADLSA010000014 DADLSA010000000
VERSION     DADLSA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.014"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..523
                     /gene="comR"
                     /locus_tag="JN691_000829"
     CDS             <1..523
                     /gene="comR"
                     /locus_tag="JN691_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAY7242342.1"
                     /translation="EATSLADLVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAK
                     HEAQLFCEEKSVESALADYFAAIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKS
                     RHAMQERTLQQFLCQRQARGEIPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQI
                     AGTTLRLWPELVK"
     gene            complement(585..1124)
                     /locus_tag="JN691_000830"
     CDS             complement(585..1124)
                     /locus_tag="JN691_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY7242343.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1351..2655)
                     /locus_tag="JN691_000831"
     CDS             complement(1351..2655)
                     /locus_tag="JN691_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAY7242344.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3063..3605)
                     /locus_tag="JN691_000832"
     CDS             complement(3063..3605)
                     /locus_tag="JN691_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7242345.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3628..4653)
                     /gene="nagZ"
                     /locus_tag="JN691_000833"
     CDS             complement(3628..4653)
                     /gene="nagZ"
                     /locus_tag="JN691_000833"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAY7242346.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDAGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4664..5488)
                     /gene="thiK"
                     /locus_tag="JN691_000834"
     CDS             complement(4664..5488)
                     /gene="thiK"
                     /locus_tag="JN691_000834"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAY7242347.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5469..6110)
                     /gene="lpoB"
                     /locus_tag="JN691_000835"
     CDS             complement(5469..6110)
                     /gene="lpoB"
                     /locus_tag="JN691_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAY7242348.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQLGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSVQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6125..6502)
                     /locus_tag="JN691_000836"
     CDS             complement(6125..6502)
                     /locus_tag="JN691_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HAY7242349.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6505..6864)
                     /gene="hinT"
                     /locus_tag="JN691_000837"
     CDS             complement(6505..6864)
                     /gene="hinT"
                     /locus_tag="JN691_000837"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAY7242350.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            6995..7195
                     /locus_tag="JN691_000838"
     CDS             6995..7195
                     /locus_tag="JN691_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242351.1"
                     /translation="MLPYISPLFPFLPANALKRHSLFGLHDKYECVYFSFAFAFAFAF
                     AFAFASKIIRTLNITAHHPPPA"
     gene            7228..9417
                     /gene="fhuE"
                     /locus_tag="JN691_000839"
     CDS             7228..9417
                     /gene="fhuE"
                     /locus_tag="JN691_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HAY7242352.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSSIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDHVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(9477..10910)
                     /gene="ptsG"
                     /locus_tag="JN691_000840"
     CDS             complement(9477..10910)
                     /gene="ptsG"
                     /locus_tag="JN691_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAY7242353.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11205..12002)
                     /locus_tag="JN691_000841"
     CDS             complement(11205..12002)
                     /locus_tag="JN691_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7242354.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12013..13017)
                     /gene="holB"
                     /locus_tag="JN691_000842"
     CDS             complement(12013..13017)
                     /gene="holB"
                     /locus_tag="JN691_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAY7242355.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPSAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13014..13655)
                     /locus_tag="JN691_000843"
     CDS             complement(13014..13655)
                     /locus_tag="JN691_000843"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAY7242356.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13645..14667)
                     /gene="yceG"
                     /locus_tag="JN691_000844"
     CDS             complement(13645..14667)
                     /gene="yceG"
                     /locus_tag="JN691_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAY7242357.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14670..15479)
                     /gene="pabC"
                     /locus_tag="JN691_000845"
     CDS             complement(14670..15479)
                     /gene="pabC"
                     /locus_tag="JN691_000845"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAY7242358.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISSDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(15599..16840)
                     /gene="fabF"
                     /locus_tag="JN691_000846"
     CDS             complement(15599..16840)
                     /gene="fabF"
                     /locus_tag="JN691_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAY7242359.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(16927..17163)
                     /gene="acpP"
                     /locus_tag="JN691_000847"
     CDS             complement(16927..17163)
                     /gene="acpP"
                     /locus_tag="JN691_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7242360.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17374..18108)
                     /gene="fabG"
                     /locus_tag="JN691_000848"
     CDS             complement(17374..18108)
                     /gene="fabG"
                     /locus_tag="JN691_000848"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAY7242361.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18121..19050)
                     /gene="fabD"
                     /locus_tag="JN691_000849"
     CDS             complement(18121..19050)
                     /gene="fabD"
                     /locus_tag="JN691_000849"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAY7242362.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALDYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19066..20019)
                     /locus_tag="JN691_000850"
     CDS             complement(19066..20019)
                     /locus_tag="JN691_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAY7242363.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20087..21157)
                     /gene="plsX"
                     /locus_tag="JN691_000851"
     CDS             complement(20087..21157)
                     /gene="plsX"
                     /locus_tag="JN691_000851"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAY7242364.1"
                     /translation="MKRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21238..21411)
                     /gene="rpmF"
                     /locus_tag="JN691_000852"
     CDS             complement(21238..21411)
                     /gene="rpmF"
                     /locus_tag="JN691_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAY7242365.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21463..21984)
                     /gene="yceD"
                     /locus_tag="JN691_000853"
     CDS             complement(21463..21984)
                     /gene="yceD"
                     /locus_tag="JN691_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAY7242366.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22183..22767
                     /gene="yceF"
                     /locus_tag="JN691_000854"
     CDS             22183..22767
                     /gene="yceF"
                     /locus_tag="JN691_000854"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAY7242367.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22879..23838)
                     /gene="rluC"
                     /locus_tag="JN691_000855"
     CDS             complement(22879..23838)
                     /gene="rluC"
                     /locus_tag="JN691_000855"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAY7242368.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24411..27596
                     /gene="rne"
                     /locus_tag="JN691_000856"
     CDS             24411..27596
                     /gene="rne"
                     /locus_tag="JN691_000856"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAY7242369.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27793..28746)
                     /gene="flgL"
                     /locus_tag="JN691_000857"
                     /pseudo
     CDS             complement(27793..28746)
                     /gene="flgL"
                     /locus_tag="JN691_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
     gene            complement(28758..30400)
                     /gene="flgK"
                     /locus_tag="JN691_000858"
                     /pseudo
     CDS             complement(28758..30400)
                     /gene="flgK"
                     /locus_tag="JN691_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(30466..31407)
                     /gene="flgJ"
                     /locus_tag="JN691_000859"
     CDS             complement(30466..31407)
                     /gene="flgJ"
                     /locus_tag="JN691_000859"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAY7242370.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKSSEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31407..32504)
                     /locus_tag="JN691_000860"
     CDS             complement(31407..32504)
                     /locus_tag="JN691_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAY7242371.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32516..33214)
                     /gene="flgH"
                     /locus_tag="JN691_000861"
     CDS             complement(32516..33214)
                     /gene="flgH"
                     /locus_tag="JN691_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAY7242372.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANVSRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33267..34049)
                     /gene="flgG"
                     /locus_tag="JN691_000862"
     CDS             complement(33267..34049)
                     /gene="flgG"
                     /locus_tag="JN691_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAY7242373.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKVQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34187..34938)
                     /locus_tag="JN691_000863"
                     /pseudo
     CDS             complement(34187..34938)
                     /locus_tag="JN691_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(34958..36886)
                     /gene="flgD"
                     /locus_tag="JN691_000864"
                     /pseudo
     CDS             complement(34958..36886)
                     /gene="flgD"
                     /locus_tag="JN691_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(36898..37302)
                     /gene="flgC"
                     /locus_tag="JN691_000865"
     CDS             complement(36898..37302)
                     /gene="flgC"
                     /locus_tag="JN691_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HAY7242374.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSAPRSYQANVEVLNTVKSMVLKTLTLGQ"
     gene            complement(37306..37722)
                     /gene="flgB"
                     /locus_tag="JN691_000866"
     CDS             complement(37306..37722)
                     /gene="flgB"
                     /locus_tag="JN691_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HAY7242375.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            37877..38536
                     /gene="flgA"
                     /locus_tag="JN691_000867"
     CDS             37877..38536
                     /gene="flgA"
                     /locus_tag="JN691_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAY7242376.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLVGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38612..38905
                     /gene="flgM"
                     /locus_tag="JN691_000868"
     CDS             38612..38905
                     /gene="flgM"
                     /locus_tag="JN691_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HAY7242377.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            38910..39326
                     /gene="flgN"
                     /locus_tag="JN691_000869"
     CDS             38910..39326
                     /gene="flgN"
                     /locus_tag="JN691_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAY7242378.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMRQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(39366..40901)
                     /gene="murJ"
                     /locus_tag="JN691_000870"
     CDS             complement(39366..40901)
                     /gene="murJ"
                     /locus_tag="JN691_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAY7242379.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41011..41934)
                     /locus_tag="JN691_000871"
     CDS             complement(41011..41934)
                     /locus_tag="JN691_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY7242380.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(41936..42583)
                     /locus_tag="JN691_000872"
     CDS             complement(41936..42583)
                     /locus_tag="JN691_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAY7242381.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNVVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42594..43178)
                     /gene="rimJ"
                     /locus_tag="JN691_000873"
     CDS             complement(42594..43178)
                     /gene="rimJ"
                     /locus_tag="JN691_000873"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAY7242382.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43414..44622
                     /gene="mdtH"
                     /locus_tag="JN691_000874"
     CDS             43414..44622
                     /gene="mdtH"
                     /locus_tag="JN691_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAY7242383.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44686..45333
                     /gene="grxB"
                     /locus_tag="JN691_000875"
     CDS             44686..45333
                     /gene="grxB"
                     /locus_tag="JN691_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAY7242384.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45467..46027
                     /locus_tag="JN691_000876"
     CDS             45467..46027
                     /locus_tag="JN691_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7242385.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46133..47179
                     /gene="pyrC"
                     /locus_tag="JN691_000877"
     CDS             46133..47179
                     /gene="pyrC"
                     /locus_tag="JN691_000877"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAY7242386.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
BASE COUNT        11419 a        12795 c        11761 g        11324 t
ORIGIN      
        1 tgaagcgact tctcttgcgg acctcgtcga agcgaccgga gccaaagcgc ccacgctata
       61 cgcggaattt accaacaaag aggggttatt tcgtgccgtt ctcgaccgct atatcgatcg
      121 ttttgccgct aagcatgaag cacagctgtt ttgtgaagag aaaagcgtgg agtctgcgct
      181 ggctgactat tttgctgcca tcgccaactg ctttaccagc aaagacaccc cggcaggctg
      241 cttcatgatc aacaactgca ccaccctctc cccagattca ggagatatcg ccaatacgtt
      301 gaaatcacgc catgcgatgc aagagcgcac tttgcagcag tttttatgtc aacgacaagc
      361 gcgcggggaa atcccgaccc actgtgacgt gacacatctg gcagaattcc ttaattgtat
      421 tattcagggg atgtcgatca gcgcacgcga aggtgcatcg ctggaaaaac tgatgcagat
      481 tgccggaacg actttgcgtt tatggcccga actggtgaaa taagtcgcaa aaaaagcccc
      541 tcagcctgag cgaatgaggg gccaaattgc agagtacagc cttgttatac tttgttattc
      601 aaaatcagtt gcccgttact atctagcggg atctgcgtac ccggatcgcg gtccatgcgg
      661 attttgccct gctgatcgcc aatcttatag gtcacatcat aaccgagcat tttttctgac
      721 ttgtcataca ccgttttaca acgctgttgc gtagtcgtgt aagtatcgct ttcctggaga
      781 gagccctgga tctggttacc ggcatatcca ccacccagcg cccccacaac agtggcgaca
      841 tctttaccgc gaccaccacc aaactgatgc cctatcacgc cacctgcaac agcgccgagc
      901 accgacccgg taatgcgatt ttcatcctgc accggtcgac gatgggtcac tgtgacgttg
      961 cgacactcct gacgcggtgt tttaaccgtt tccttgattg gggttgcaga aacaacctga
     1021 gcgtattgcg ggccccgttc aaacacgttc agactggcca ctgccgctac gcccaacgca
     1081 gctgcgacac caatcccgat acccgccaac attgatttat tcacgggaac acctccttct
     1141 ttgcagttat gccaatcatg caagcgaagg agaaaagcag ctatcagaca gaagatgaaa
     1201 agcgcgggaa tgactggaaa tacttaagga ttcggaggag tctgaaggaa tgcctgatgc
     1261 gacgcttaac gcgtcttatc aggcctaccg ctcgtaccat ttgtccaggg cggataaggc
     1321 gtttatgcca catccgccag tgtacgtcga ttaatgcaac ttcaaacgcg gacggataac
     1381 gcggttaata ctccccacca gcatcattaa tccggtttta aagtaaccat gcagcgcaat
     1441 ctgatgcatt cggtatagcg agatatatac aaagcgcgca attcgtcctt caatcatcat
     1501 tgagccgcgc gtcaggttac ccatcaggct accaacggtg gagaagttcg acagcgatac
     1561 cagcgaacca tgatctttat actgataatt tttcagcggc ttaccgttca tctgcgccag
     1621 aatgttgttc attgcgcaag tcgccatctg gtgtgcagcc tgagcacgcg gcggaacaaa
     1681 gcccccttcc ggacgcgggc atgacgcgca gtcgccaata gcgtaaatgt ctggatcgcg
     1741 agtggtttgc agcgtcggtt ccaccaccag ctggttgata cggttagttt caagaccacc
     1801 gatatctttc aggaagtctg gcgctttgat cccggctgcc cataccatca gatcagcctc
     1861 aatatattcg ccatctttag tgtgcaggcc gccttcatca gcactggtga ccatggtttg
     1921 cgtcagcacg cgaacgccaa gtttcgttag ctcgttgtgg gccgcagcag agatacgtgg
     1981 cggtaatgca ggcaaaatac gttctcccgc ttctaccagc gttacgttca gggcttcgtt
     2041 ggtcaggcct ttgtaaccgt agctgtgcag ttgcttgacc gcgttgtgca attcagcgga
     2101 gagttctaca cccgtcgcgc cgccgccgac aatcgcaatg ttcactttgc catttgcgcc
     2161 caggttggca gagtatttca ggaacagatt cagcatctcc tggtggaagc gacgcgcctg
     2221 gtgcgggtta tcgaggaaaa tgcagttctc tttgacgcct ggcgtattga aatcgttaga
     2281 ggtgctaccc agcgccatga ccagggtgtc ataggcgatt ttacgttccg gaaccagcag
     2341 ttcacctttc tcgtcgcgta gttctgcaat agtgattgtt ttcgcttcac gatcaatatc
     2401 aatgacggaa cccaactgga actggaaacc atgattgcgc gcatgggcca gatagctcaa
     2461 cgcatcgacg ccttcatcaa gcgagccagt cgccacttcg tgcagcagcg gtttccacag
     2521 gtggctgtgg ttacgatcga ccagcgtaat tttggctttt ttcttacgtc ccagcttatg
     2581 ccccagctgt gttgccattt ccagcccacc agcaccgccg ccgacaatca caatcttttt
     2641 caatggcgta gtcaacgtga cccccttaaa tttattaacc aattgttaat taatggttat
     2701 taacatagct cttaattaac aacaggttac agcaatgaaa atgttcttcg ctattgagaa
     2761 taacatgaat ggtgcattgg tcataccaaa attgatgtta gtcaagtttt gttgcttaaa
     2821 agataggcaa ctttaccggg tgagatcaaa aaatagagtt tattgtttat tatttgagca
     2881 aattcggaag agtaatatag ggtgaagcgt atatcgaggg cgtttggtcg tcctgataag
     2941 atgcgcagca tcgcatcagg cattgatgct cgactgtcgg atgcggcgtg gacgccttat
     3001 ccgacttaca gaattcggag cgcatcaggt accggcaagg ttacctgatg cgacgagggc
     3061 atttacccga gggttttgaa cgctttaatg cgctgtaaat gcggggagat attcttgaat
     3121 ttgtgcgtct gctcttcgtc ccagacaatc tcgtaataat gatgcaactc ttcagatgtc
     3181 cgctggctgt taagtgcttc atcattacgc gacaaaataa ccagacaacg atcgcgattc
     3241 ttctcgcgga agttggtcac acacttagtg gcaatatcgg catactcttc cgggcgatca
     3301 atcttgcctt ccatgttctc ataagggaac aaattagggt tgaagatcac ctggcggata
     3361 tcgcaaagaa aaccaatccg ttccgcccag tatccgccca ggccaacgcc gcaaattagc
     3421 ggacgctcgt caacgttcag ttgcaacatt ttgtccactt ctttaagcag atgctgcata
     3481 tcatgtttcg gatgccgcgt actgtagctt atcaagcgta catccgggtc aataaactgc
     3541 aattgtaaga ctttctcgtg gttacccgga ctgttagagt caaaaccgtg taaatagata
     3601 atcatcgctt cctcacataa gccagggtta gtgacctgct ttctcttcct gccagcgttc
     3661 atgtaactga ttcagacggg tgctgatcgc tttccagcga gccgagtcca tcagttcctg
     3721 tcgcgaaaat gaacctttat gatacaaacg tgtaacacgt tctgccttga tcggtgacag
     3781 attatctaac acgctgacgg cccctttacg attattgcag accaggatca tatcgcaacc
     3841 cgcatccagt gatgcctgcc cgcgttcggc ataactgccc ataatcgcgg caccttccat
     3901 cgataaatcg tcagagaaaa tcacgccgtc aaaacccagt tcctgacgca aaacggtttt
     3961 cagccagtag ggagaaccgc tcgccggacg cggatcaaca tcactgtaga tcacatgcgc
     4021 aggcataatg gcgtcgagtt tattttcgcg gattaaggaa ctgaagaccg acatatcttt
     4081 agcgcgaatc tccgcttgtg gacgtggatc gcacggtgtt tctttgtgtg agtctgccgt
     4141 tactgcaccg tgtcctggga agtgtttccc ggtcgttttc attccggctt catgcatacc
     4201 atcaataaac cggctggcaa ttgccagggc tttttgtgga tcggcatgat aagaacgctc
     4261 gccaatcgcc gcgctgatat gcccggcatc cagcactggc gcaaagctga tatcaatatc
     4321 catagcgatc atttcactgg ccatcagcca acccgcctct tgcgccagtt tgccgccctc
     4381 ttccattccc aacagcgcag caaaggattg tgctgccggt aagcgggtaa aaccttcgcg
     4441 aaaacgctgc acgcgtccac cttcctgatc taccgccacc accagatgat tgcgcgatgc
     4501 tgcgcggatc tggcgcacca gttcacgtaa ctgggcagga tcatgatagt tacgcgtaaa
     4561 gagaatcagc cctcccacca gcggatgcgc cagtatttca cgctcttccg cgtccagttc
     4621 gtaaccttcg acatccaaca ttactggacc cacactgctc tccttattgt tttattaata
     4681 gctgccgcca ggtgtcatcg gccagcctga taaattgttg atcgccggtt tgtcgccagc
     4741 ggtactcaaa ccaccctcct ttgagcatca gcagccaggg aaaccatcgc ctgacctgac
     4801 gccataattg cgccggataa atcttcgcgc gagtggcata gtcattgacc aattgccggt
     4861 gctgttcagt attttccacc cacaccgccg ccagttccag cgcgatatca ccatctccgg
     4921 catactccca gtcgatgagt tttaaccctg atgcgctatg cactaaattt ccggcgtgga
     4981 catccatatg caatggactt aagcgtaaag gtcgtggttc ccgcgctttg cgcagacgtt
     5041 ttaacattcg cagccaaccc acagtccgcc gcgccggatc gctttgctgc cagtacagtt
     5101 ccagtaaggg caacagcgtt attcgccagc caaaacgtgg ttgttgatgt agataataca
     5161 gcaagcctgc cagttcgttg gtatccggca aatacgtttt taccgcgccg ggcagatagt
     5221 cgactaccat ccagtcacgg agatataaat gcggcttcgg tgcaatgctt gcgggtagtt
     5281 gtgataaagc ccgatactgg cgcaagaacg cggactgcgg cgcatcagga tcgtgcggct
     5341 gacgcacaac aaaacgctgg ccctgatgtt caatgagaaa actaccgcca ctaagcccac
     5401 tattaaacgt cgtgacggga tgaaactgcg ggaaaaagcg cgacagcaat tcgtcgcgcg
     5461 tgatgggatt attgctgcga aacggcacct ttacctgacc agataatttc gcccgtctgc
     5521 accagcatca gctgcatttg tagggtcgga gcgttaacgt tgccagaggc gctggagtac
     5581 agcacgtaat gagcgccgac attgcgggca atgcctatgg ctttactacg ggtgcctaaa
     5641 ctgtcctgcg gcgacaaacc taactgttgc ttagccatcg acagttgctg gacggaaacc
     5701 agggtaaatt tcccgttatt agccagcgca tttcgcagcg tttcggtcgc ttctgcggca
     5761 ttcagcgaac cgttagtacg gttgttaacg ctatcaacca gcaggacgct acccgcagtc
     5821 accccgtcag ccccaagcat cttactgacc atcggctgca ttgcgccatt ccagtcataa
     5881 tggcgaatat gcggcgcagg cggtgccgtt cgatcttcgt gctcaattgg gcctagctgc
     5941 tgcgggatcg tcggcaccga gggcactgtg gggacaggct gttgtggctc ggctggttgt
     6001 tccggcgctg gtttcacttc ttctaccggt gcaggttcac gttgccccac acacccggcg
     6061 agaaacatcg ccagcgcggt aatcaaggcg tagcgactca tttttgtcat caagattcac
     6121 ccctttacaa atatagataa agtctgactt tgtgcgcccc cagaaaattg gcgctgccgt
     6181 acagcgttac cgccgaatgt gcgggaatgg taacgctgcg tggcctttcc agaggatgca
     6241 tctccagccc tctggcgtca taccagtaaa aacgataatg aacggtaacg ggttcttgcc
     6301 tttcgttata aagcgttgag gatgctgaag gttgaatatc agacgtcgaa aggacgggct
     6361 tttctgcact gatgcccgca gccaataaag atgactccat caccagcgat tgctcatcat
     6421 tcaccggaat ttccggatgt gaacgacagc ccaccagcag taacaacacc agagacacca
     6481 gcccaaagca tccttttctc atcgttacag acctttatgc gccagcattg gtcccagcgg
     6541 acggccaccc aacaagtgca tatggatgtg gtaaacctct tgtccgccat ggcggttggt
     6601 attcatgatc agacgatagc catcttcggc aataccttct tgctcagcaa tttttgccgc
     6661 tacggtgatc atgcgtccca gcgcctgctc atgctcagct gacacgtcgt tcacagtcgg
     6721 aatgaggata ttcggaatga tcagaatatg cgttggcgct tgcggcgaaa tatcgcgaaa
     6781 cgccgttacc agatcatcct ggtagacgat atcggagggg atctcacgac gaataatttt
     6841 gctgaatata gtttcttctg ccacgacatt ttcctttttc aaatagccat gcgttgcgcc
     6901 atgctacgcc gcgccacact gcgagtatag agtatggtcg agttaaacct gctctttcaa
     6961 ccttaactca cgatttctta agcaaaaaat cgttttgctg ccgtatatat cgccattatt
     7021 cccatttctg cctgcgaatg cgctgaaaag acattctctg tttggtttac acgacaaata
     7081 tgaatgcgta tatttctcat ttgcatttgc atttgcattt gcatttgcat ttgcatttgc
     7141 aagcaaaatt attcgcacac taaatataac cgctcatcac cctcctccgg catgagcctg
     7201 acgacaacat aaaccaagag atttcaaatg ctttcaacac aatttaacag ggataatcaa
     7261 tatcaagcca tcaccaaacc gtcactactt gccggttgca tagcactggc actattacct
     7321 tctgccgctt ttgctgcacc agccactgaa gaaacggtga ttgttgaggg ttcagccaca
     7381 gctccagatg atggcgaaaa tgattacagc gtaacgtcta cctctgcggg taccaaaatg
     7441 cagatgactc aacgtgatat tcctcagtcg gtcactattg ttagccagca gcggatggaa
     7501 gatcagcagt tacaaacgct gggcgaagtg atggaaaaca cgctggggat cagcaaaagt
     7561 caggcggatt ccgatcgtgc tctttattat tcccgcggat tccagatcga taactatatg
     7621 gttgatggta tccccaccta ttttgaatcg cgctggaatc tgggcgacgc actttctgat
     7681 atggcactgt ttgaacgcgt agaagtagtg cgtggcgcga caggactcat gaccgggacg
     7741 ggtaatccat ctgcggcaat taatatggtt cgaaaacacg cgaccagtcg tgaatttaaa
     7801 ggcgatgtct cggcggaata cggtagctgg aacaaagaac ggtatgtggc tgatttacaa
     7861 agcccactca ccgaagacgg taaaatccgc gcgcgaattg tcggcggcta ccagaataac
     7921 gactcatggc tggaccgcta caatagtgaa aagaccttct tctcgagcat tgtcgatgct
     7981 gatttaggcg atcttacgac gctttcagcc ggttacgaat atcagcgcat tgatgttaat
     8041 agcccaacct ggggcggttt accgcgctgg aatactgatg gcagcagcaa cagttacgat
     8101 cgcgcacgca gtaccgcacc tgactgggcg tacaacgata aagagatcaa caaggtcttt
     8161 atgaccctga agcagcggtt tgctgatacc tggcaagcga cactgaatgc cacccactct
     8221 gaagtcgaat ttgacagcaa aatgatgtat gtcgatgcct atgtaaacaa agcggatggt
     8281 atgctggttg ggccatacag taattatgga cctggctttg atcatgtcgg cggcaccggt
     8341 tggaacagtg gcaaacgtaa agttgatgcg ctggatttgt tcgctgacgg tagttatgaa
     8401 ttgtttggtc gtcagcacaa tctaatgttt ggtggcagtt acagcaaaca aaacaatcgt
     8461 tacttcagtt catgggccaa catcttcccg gatgaaattg gcagtttcta caactttaat
     8521 ggcaatttcc cacaaaccga ctggtcacca cagagcctgg cgcaggacga taccacacat
     8581 atgaaatcgt tatatgctgc cactcgtgtc acccttgccg atccgctgca tctgatcctc
     8641 ggcgcacgtt ataccaactg gcgggttgat acgctgactt acagcatgga gaaaaaccac
     8701 accacgcctt acgctggtct ggtgtttgac atcaatgaca actggtcgac ctacgccagc
     8761 tatacctcta ttttccagcc gcaaaatgat cgtgacagtt caggcaaata tctggctcca
     8821 atcaccggta acaactacga gctgggtctg aaatcggact ggatgaatag ccgtctgacc
     8881 accacgttag ccatcttccg tattgagcag gataatgtcg ctcagtccac cggtacacct
     8941 atccccggca gcaacggcga aaccgcctat aaagcggtgg atgggacagt cagtaaaggg
     9001 gtggaatttg aactcaacgg cgcaattacc gacaactggc agctgacatt tggcgcaacg
     9061 cgctatattg cagaggataa cgaaggaaac gccgttaatc ctaatctgcc acgcaccacg
     9121 gttaaaatgt tcaccagcta tcggttgcct gtcatgccag agttgacagt cggcggtggt
     9181 gttaactggc aaaatcgcgt gtataccgac accgtgacac cgtatggcac cttccgcgcc
     9241 gagcaaggta gctacgcgct ggtggatctc ttcacccgct accaggtgac gaaaaacttc
     9301 tcgttacagg ggaacgtcaa taacctgttc gacaaaacct acgataccaa catggaaggt
     9361 tctatcgtct acggcgcacc gcgtaatttc agcattaccg gcacgtatca attctgaata
     9421 acacctgtaa aaaaggcagc catctggctg ccttagtctc cccaacgtct tacggattag
     9481 tggttacgga tgtactcatc catctcggtt ttcaggttat cggatttagt accgaaaatc
     9541 gcctgaacac cagaaccagc aaccactacg cccgctgcgc ccagtttctt caggccagcc
     9601 tgatccactt tagacacatc agcaacgctg acgcgcagac gggtaataca tgcgtcgagg
     9661 ttagtaatgt tttctttacc accaaatgca gcaaccagag ccggtgccat ttcgctggta
     9721 cctgttgctt ttgcatcttc agtcgcgtct tcacgacccg gcgttttcag atccagtgct
     9781 ttaatcagca cgcggaagat ggtgtagtaa acaatcgcat aaccgatacc gacgatcggg
     9841 aacagccaca gtttgctgct gttaccagac agaacgatga agtcgatcag accgtgcgag
     9901 aacgacgtac cgtcacgcat ccccagaaga atacagattg ggaatgccag gcctgccaga
     9961 atcgcgtgga tgatgtacag gatcggcgca acgaacatga agaagaactc gatcggctcg
    10021 gtgataccgg tcaggaacga ggtcagcgcc gcggagatca taataccgcc cactttcgcg
    10081 cggttttctg gtttagcaga gtgccagata gcaattgcgg cagctggcag accgtacatt
    10141 ttgaacagga aaccaccaga cagtttaccc gcagtcgggt cacccgccat ataacgcgga
    10201 atgtcgccgt ggaaaacctg acctgctgcg ttggtgtatt caccaatctg catctggaaa
    10261 ggtacgttcc agatgtggtg cagaccaaac ggtaccaggc aacgttcgat gaaaccgtaa
    10321 atgccaaacg caactaccgg gttctggtaa gcagcccact gagagaaggt ctggattgca
    10381 gaaccaatcg gcggccaaat gaaggacagc acaacgccag taaagatggc agccaggcca
    10441 gaaatgatcg gcacaaagcg tttaccggca aagaagccaa gatactcagg cagcttaata
    10501 cggtagaaac ggttaaacat gtacgctgcg atcgcaccgg agataatccc tccgagtacg
    10561 ccagtatccg ccaagtgttt agaggcgatt tcttcagcag gtaaatgcag taccagtggc
    10621 gcaaccacgg ccatggtttt aaccatgatg ccataggcaa caactgcggc cagcgcggat
    10681 acgccatcgt tattggtaaa gccgagggcg acaccgatcg caaaaatcag tggcatgttt
    10741 gcaaagacgg aaccgcctgc ttctgccata acatgcgata caacggcggg cagccagctg
    10801 aaattcgcgg aaccgacgcc cagcagaata cctgcgatag gtagtacgga taccggcagc
    10861 atcagcgatt taccgacctt ttgcaggtta gcaaatgcat tcttaaacat aattgagagt
    10921 gctcctgagt atgggtgctt tttttacgtt ctcacgcgtg gcaagggggg agagcctcgc
    10981 cgtgtacagg gcatctaagc gccctttatt tattacacag agtaaaataa ttcagtgcca
    11041 atttgtttga cggctatcac gtttcaactt cagatcattg gagaagttgc acattttgac
    11101 aaaacatatg tcacaatatt tcaaacgcag ttattcaccg cccaattttc ggcggtgaac
    11161 tttactcgtt ttagccatta attacgagct ttaaaaaaac tcattcaacg gatggattga
    11221 aggcgggaag cgtcgatgtg aaacagacgg gcgaagttat cggtggttac ctgcgccagt
    11281 tcttcaacgg caacaccttt caacacagcc atgtattctg caacgtcacg aaccatcgca
    11341 ggttgattct cttttcctcg atgcggtacc ggcgcaaggt aaggtgagtc agtttccacc
    11401 agtaatcgat ccagggggac ataacgcgca gcatcgcgca gttgctccgc attacggaag
    11461 gtcacaatgc cggaaaagga gatgtaaaat ccgagatcca gtaatttacc cgccgtttct
    11521 ctgtcctctg taaaacagtg tagtacgcca ccgcaatccg tcactttttc ttcgcgcaga
    11581 attgccagcg tatcggcacg ggcgtcacgg gtatggacga taaccggctt gttcagttca
    11641 cgaccaatct ggatatgatg gatgaaggac tcttgctgac gtactttagt ttccggtgtg
    11701 taataataat ccagcccggt ttcacccagc gctacaacac cctcttctgc cgccagacgg
    11761 cgtaaatctt ctacatcgta gggatcattc tggttgagtg gatgcacgcc acaagaaaat
    11821 acgacgttgt cacgttcgcc taccagatcc cgcatatgta aataacccgg taatgttgtg
    11881 gcgactgcca gacaaaattt cacatcgcgt gcggcggctt tcgccagaac gtcatccacg
    11941 tccttatgca aagattcata atccagacca tcgagatggc agtgtgagtc gactaaaaac
    12001 atgatgtctc tcttaaagat gaggaaccgg tagcacaacg cccggttgca ggtaatgctc
    12061 aatacgcagt aaaagatcgg tgatgagaag ctcgcggttg atgcctgtga cagacattaa
    12121 ctgttcacga atgtggcaaa catcccccag tatagcctgc aggcgcgagg gagaaagatg
    12181 gtttgccagt tcggcgacca ggcccggcac atcaacattg gtcacctgcg cagcgccatg
    12241 atggcgtttt agcgcatcca tcagcaacgt tgccagccag tgtaaacgcg ccggagcttg
    12301 ttcatgatta agggccgcca gcagcgaata ccagtcgccc gatggcacgc tatatgccaa
    12361 cgcctgacac aatgtttcac gagcctgcca gttatctccc tgaaacaacg ccagtgccgc
    12421 gctaggcgaa ccggcgctta agcgcaatgc ggcaagtaat gcatcctgtg acattgtcac
    12481 ttcgcgtgaa agccaagtca cggcgtactg ttctggaggc ggcgcaaggt aatgtaaccg
    12541 acaacgacta cgtaatgttg ccagtaaacg ttcaggctcg cgggtagcca ggaaaaacca
    12601 agtttctgct ggtggctctt caagcgtttt cagcaatgcg ttagccgcgg cgtcggtcag
    12661 taaggcagca tcggttaccc agacaacttt cgcaccacct aggcgtgcgt gctcattcag
    12721 cttttcggtg acctcacgta ccgcatcaat gcccagcgta tttttccctt tttcgggagc
    12781 cagggtgtag taatcgggat gcgtgccagc ctgcatcaac tgacatccac gacagtgacc
    12841 gcaacttttg tggccctgcg gttgttggca gagtaaataa cggctcaggg cgtagattaa
    12901 agcatcatcg cccatgcccg gtaacgcctg aatgagtagc gcatggtgac ctcttccggc
    12961 ctgatagctg gctaccagtt tttcgaaatc aggtcgtaac catggatacc atctcatgcg
    13021 tccaactcct tcacccagtg ggtcacggta gtgcggattg catccatcac ggcctccagc
    13081 ggctgggtgg catcaatggt atgaatactt ttatcttgtg cagccagttc cagatagcgg
    13141 gcgcgggtgc gattaaagaa atcgaaagac tcttgctcaa tacgatccag ctcgccgcgc
    13201 gcacgcgcgc gttttaagcc aacttccggg gtaacatcga gatagagcgt taagtcgggg
    13261 cgaaaatccc cgagaacagc atcacgcagt gttgccagca tatgttggtc aataccacgt
    13321 cctccgccct gatacgcctg agtggagaga tcgtggcgat cgccaatcac ccaggtgccg
    13381 ttagccagcg caggtttgat gaccgtttct accagttgaa cgcgcgcggc ataaaacatc
    13441 agaacttcgg ctttatcggt aatgacttca tcgcctaccg atttgatatc cagcaccagg
    13501 cttcttaact tttcggcaag ttgcgtaccg ccaggttccc gagtgaaaac catgtcgcgg
    13561 atacccagtt gctcgagcgt ctcaaccacc acattacgcg cggtagtttt gcctgcgcct
    13621 tccagcccct caatgacgat atacttactg cgcatttttt tccttaagca ctttcagata
    13681 atcctgcaca gacttgttat gactggcaag attggtatta aacgtgtgac cacctttacc
    13741 atcggccaca aaatagagat acggcgtttt tgccggatgc gcagcagcct tcagcgaatc
    13801 cgcccccggc gtagctatcg cacccggcgg caaaccggta atggtatagg tgttatacgc
    13861 tgtcggcgtt tccaggtctg cacgagaaag tttgccatta taacgctctc ccatcccgta
    13921 aatcacggtt gggtcggtct gcaggcgcat accaatgcgt aaacggttga taaataccga
    13981 ggcaacctga tcgcgttcac tggcaacggc ggtttctttt tcgataattg atgccatcgt
    14041 caccagctga tttttatctt tataaggcag accgtccgca cgcccttccc aggcgctatc
    14101 gaccgctttc accattttct tgtgagctcg cttgagtaac gcgacatcgg tggtattggc
    14161 ggtatacatc aaggtgtctg gccagaacca accttcaatc cactccgggt tttccagttc
    14221 aagtgcctgc gctacggtgg cgtacttatc gtcactcagc gtgtgcttga tatacggagc
    14281 ctcacgcaat tgcttgaggt agtcgctcag acgcatccct tctaccagtc gcagcgggaa
    14341 ctgtgcttct ttaccgcttt ccaacaattt cagcatctcg cgcacggtca tctgcggtgt
    14401 aaagcggtaa gtcccggctt taaagtgaga aagatccggt tcgatacgca gcagccattg
    14461 aaaaacccgt gggcgattga tgatcttatc agcataaagc tgttcaccga gcgccagacg
    14521 tccggtccct ggcttcaggg taaatatcgt ctcttctttg ataagcaatt tgctgtcggc
    14581 aagatggcga accttccaga cgcccacacc agcggcgata cccagtacca ccagcaataa
    14641 caagataatc aataacactt ttttcatgac taattcgggc gctcacaaag tggggctaaa
    14701 tattcatata acgttgctga cgaaaaggtg acatcgccac aggcacgtac gggcatcact
    14761 ggcattaacg cattacaaat aaccatctca tctgcctgca acgcctcctc cagcgaggct
    14821 tgcacttcga caagctgata agaggattgt gccagtaaac ggatacagaa ttgtcgcata
    14881 atgccgttaa cacctgcctg atccagtcgc ggcgtataaa ctacattgcc cttccgccag
    14941 aacaaattag ccgcacagca ttccgtaacc cacccttcgc tgtcaaggac cagcgcctca
    15001 tcagcgtttg tctgctcaag atgagagcga atcaatactt gctcaagccg attaagatgt
    15061 ttaatacctg caagatgagg attgcgcccc agccgcaccg ggcttagcgc caacgtcatc
    15121 ccttcgttac gcaaacggtc gtaatgtgca ggataagccg taacggagag aatccgcgtt
    15181 gctggtccgc tgttcaatgt gctgtaccct cgcccgccac taccgcgact gatcacgact
    15241 ttcagcacac cattttgctg ttctgctgcc agcgttttca tctcttgttc aagctgaggc
    15301 cagaagtcac tggaaatcat caaccgctga caagcatcct gtagtcgctg gatatgcgcc
    15361 gataacaaac tgactttacc gtcgataact ctggcggtgg taaaacaacc atcaccaaac
    15421 tgcgttgccc gatcgcttac tgccagcgat tcctgcttat aaccgttaat taagaacata
    15481 ccggctcctt atcagagaat tgttagtgtg gcagcatgtt cactacggaa caagtcggaa
    15541 taaaagctaa gaaaaaaggc ccgcaagcgg accttttata tgggtggaaa atgacaactt
    15601 agatcttttt aaagatcaaa gaaccattag tgccaccgaa gccgaaggag ttacacagcg
    15661 tgtattccat tccgctgacc tgacgtgctt catgcggtac gaaatccaga tcgcaacctt
    15721 catccgggtt atccaggttg atggttggcg gaacagcctg atcgcgcagc gccaggatgg
    15781 agtagataga ttctactgca cccgccgcac ctaacaggtg accggtcata gatttcgtgg
    15841 agcttaccaa cacacggctt gcagcttcac cgaagatggt tttcaccgcc tgcgcttcag
    15901 ctttatcgcc agccggcgta gaagtaccgt gcgcgttaac gtagccaatc tgactcgctt
    15961 caatgcctgc atcacgcaga gcatttgcca ttgccagagc tgcgcctgcg ccattttctg
    16021 gcggtgacgt catatgataa gcatcgctgc tcataccaaa gccgacgagt tcagcgtaaa
    16081 ttttcgcacc gcgttttttc gcgtgttcgt actcttcaag taccagcata ccggcaccat
    16141 cgcccagtac gaagccatca cgctctttat cccacgggcg gctcgccgct tgcgggttat
    16201 cattgcgggt agataatgca cgtgccgcgc caaaaccacc aacgcccagc ggcgtactgg
    16261 ctttctctgc gccacctgca accatcacgt cagcatcgcc atacgcgata atacgcgcag
    16321 catggccaat gttgtgcacg ccggaagtac aggcggtcgc gatagagatg cttgggccac
    16381 gcaggccata catgatagtc agatgacctg ccaccatgtt cacaatcgtt gacggaacga
    16441 agaatgggct gatcttacgt ggaccaccgt tcatcagaga tgtgtggttt tcttcgatca
    16501 gtccgaggcc gccaatcccg gagccaattg cggcaccaat gcgggttgcg ttctcttccg
    16561 ttatttcaag gccagaatcc tgcatggcct gaacgccagc gacaattcca tattgaatga
    16621 aggcatccat cttgcgctgt tctttgcgcg agataatgtc ctcacagtta aaatccttta
    16681 ctaagccagc aaatttcgtt gcataggcgc tagtatcgaa atggtcgatt aggctgatgc
    16741 cactctgacc ggcaagcaga gctttccagg tagactctac ggtattgccg acaggagaca
    16801 acatgcccag tccggtcaca actacacgac gcttagacac gtttgtcctc cagggaggga
    16861 aaaatgattc tagtgggaca aaaagataaa actcaggcgg tcgaacgacc gcctggagat
    16921 gttcacttac gcctggtggc cgttgatgta atcaatggca gcctgaacgg tggtgatttt
    16981 ctcagcttct tcgtccggaa tctcagtatc aaactcttct tccagagcca ttaccagctc
    17041 aacggtgtca agagaatccg cgcctaggtc ttcaacgaaa gaagcattgt tggtaacttc
    17101 ttcctgctta acgcccagct gttcgccgat aattttctta acgcgttctt cgatagtgct
    17161 catactctta aatttcctat caaaactcgc tttcgcgatg gttttcgtag tgtataaaat
    17221 gttgaaaaat ttgcaactaa atcccggcag gtcttaccac gattttacgt tattttgagg
    17281 ccttcccccc caataacgca aatcattttg cactaattat tactcataac cacgcagact
    17341 gcgcgcaaca tgagcatttt gtgcaaatcg cggtcagacc atgtacatcc cgccgttcac
    17401 atgcaaagtt tcacccgtga tgtaagctgc ttcgtcggat gccaggaatg caaccgcgtt
    17461 ggcgatttcc tgtgcgccgc cgaggcgacc cgcaggaacc tgcgccagga tacccgcacg
    17521 ctggtcatcg ctcagcgcac gtgtcatgtc cgtttcaata aagcccggag caacaacgtt
    17581 tacagtaata ccgcgtgacg caacttcgcg cgccagtgat ttactgaagc cgatcaagcc
    17641 cgctttcgcc gcagcgtagt tggcctgacc gccatttccc atggtaccaa ccacagaacc
    17701 gatagtgata atacgaccat gacgcttttt catcatagcg cgcattaccg cttttgacag
    17761 acggaaaaca gatgaaaggt tagtttcgat aatatcgttc cactcttcat ctttcattcg
    17821 cattaacagg ttatcacgag tgataccggc attattgacc aggatatcca cttcaccaaa
    17881 ttctgcgcga attttttcca gaacagattc gatagatgcc gggtcggtca cattcaacat
    17941 cagacctttg ccgttggcac ctaaataatc actgatcgcc tgagcgccat tttcactggt
    18001 cgcagtgcca ataactttcg cgccacgggc tgcgagcgtt tcagcaattg cgcggccaat
    18061 tccgcggctt gcaccggtta ccagtgcgat ttttccttca aaattcatga ttttcctctt
    18121 ttaaagctcg agcgccgctg ccatcgctga aggttcgttc agcgccgagg cggtcagggt
    18181 gtcgacaatg cgtttcgtca ggccagtaag caccttgccc gggccgactt catagagatg
    18241 ttctacgcct tgcgctgcca tgtactcaac agacttcgtc cactgaaccg ggttatacaa
    18301 ctgacgtacc agtgcgtcac ggatggcatc accattggtt tcgcatttca catcaacgtt
    18361 attcacaaca gaaactgttg gtgcgttaaa ggtgattttc gctaattcta ctgccagttt
    18421 gtcggctgct ggtttcatca gcgcacagtg agacggtacg ctcactggta acggcagcgc
    18481 gcgttttgcg ccagcagctt tacaggcagc gccagcacgc tcaaccgctt ctttatgacc
    18541 ggcaataacc acctgtcccg gagagttaaa gtttaccgga gaaacgacct gaccttctgc
    18601 agcttcttca cacgctttcg caatagacgc atcatccaga ccgatgattg ccgccatagc
    18661 gcccgtgcct tccggtacgg cttcttgcat gaacttaccg cgcatctcaa ccagacgcac
    18721 cgcatcagcg aaatcaatca cgccagcgca aaccagcgcg gagtattccc ccaggctgtg
    18781 accggccatc attgccggtg ctttaccgcc ctgctgctgc catacgcgat acagcgcaac
    18841 agatgcagtc aacagcgctg gctgggtttg ccaggtttta ttcagttctt cagctggccc
    18901 ctgctgggtc agcgcccaca ggtcgtagtc cagcgccgca gaagcttcag caaacgtttc
    18961 ttcgacaatt ggatagctcg ccgccatatc agccagcatt ccaacggttt gagaaccctg
    19021 tccagggaac acaaatgcaa attgcgtcat gttttaatcc ttatcctaga aacgaaccag
    19081 cgcggagccc caagtgaatc caccgccaaa ggcttcaagc agaaccaact gccccggctt
    19141 aatgcgcccg tcgcgtacag cttcatccag cgcgcacggg acagaggccg cagaggtatt
    19201 accgtggcga tccagcgtca ccacgacatt gtccatcgac ataccgagtt ttttcgccgt
    19261 tgcactgata atacgcaggt tagcctgatg cggaaccagc cagtccagtt gagaacggtc
    19321 aagattattc gccgccagcg tctcatcaac gatgtgcgcc agttccgtta ccgcaacctt
    19381 gaagacttcg ttgcccgcca tcgtcagatg aattgaattc tctggattca cgcggtcggc
    19441 gtttggcagc gtcagcaatt caccataact accgtcggca tgcagatggg tggaaatgat
    19501 tcccggctct tcagaggcag ccagcaccgc agcgcccgcg ccatcgccaa aaataataat
    19561 agtcccacga tctgttggat cgcaggtgcg cgccagtaca tcggaaccga cgaccagagc
    19621 atacttcacc gccccagatt tcacgtattg atcggctacg cttaatgcat aggtgaaacc
    19681 tgcgcaggct gctgcaacgt caaatgccgg gcaaccttta atgcccaaca tgctttgaat
    19741 ctgacaagct gcgctcggga aagcgtgcgt agcagaagtc gttgccacaa cgatcaggcc
    19801 aatctggtct ttctcaatgc ccgccatctc aattgcgcgt gtcgccgctt caaagcccat
    19861 ggttgaaacg gtttcgtttg gcgcggcaat gtggcgttcg cggataccgg tacgagtgac
    19921 aatccactcg tcagaggtgt ccaccatttt ttccaaatcg gcgtttgtcc gcacttgttc
    19981 gggcagatag ctgccagtac caataatctt cgtatacatg tacgctcagt cacttttcgg
    20041 ttatataccg tcacttgcaa actgcgagtt cgctggcagc gtcctgctac cgcagagttc
    20101 cgcttttgcc accgtccagc agctcaaaac cagctgggta tacagattcc aggcgagcgg
    20161 caattcgctg aggaacttgt cgctgcaccg cctgcactgc ctgttcaatc gcgaccgcaa
    20221 aagctcgctg attggctgca ccatgacttt ttatcaccgt gccgcgcaat cctaacagac
    20281 aggcgccgtt atactggtcg gggttgaggt gactgaatcg cctcgtcagg ctcttttgta
    20341 gccaacgctt taataacagt agccaccacg accgtttttt cccttcaccc tgagatttca
    20401 gcagagaaag gaacatcctg acaacacctt ccatcgtctt taacgtgaca tttcctgtaa
    20461 agccgtcaca aaccagcaca tctgtcttgc cagttaacaa ctcattggct tcaagatagc
    20521 cgatataatt gatagaaggg attgttttaa gcaccgctga ggcatcccga atactgtcga
    20581 gaccctttac ttcttcttca ccaatattga gcaacgccac gcgaggattg ggaatttcca
    20641 ccacctcttc agccagaact gagcccataa tggcaaattg caccaacatt gtgctgtcac
    20701 aatcgacgtt ggcccctaaa tcgaggacca ccgttttgcc cttttgctga tgtggtaata
    20761 ccgtcaccag cgccggacgc tcaatcccct ccaggggctt gagtaataat tttgccagcc
    20821 ccatcagtgc cccggtatta ccggcactga cacaggcttg cgctcgacct tctttcacca
    20881 gctccagggc catacgcatt gaactcccac gactggcgcg gatagcttgc gaaggccggg
    20941 catcactggc gataactgac tgcgcaggaa taatctgcag acgcgaacgt tgttcaaagt
    21001 cagctttagc aagtaatggc gtgatggcgt cgggattgcc gactaaaaga agagtgagtt
    21061 gcgaattaga attcagtgcc tgcaatgctg caggcactgt cacggaaggg ccaaaatccc
    21121 ctcccatgac atctaacgcc agggttagac gtttcaaggt atcgtcgccg cccggtttgg
    21181 tctttcccca gttgcctggg gaaaatcctc actaagcttc atcacgcaga cgcgtgatta
    21241 cttagcgatg accttgcggc cgcggtagta accgtcggca gtgatgtggt gacgcaggtg
    21301 tttttcacca gaagttttgt ctacagacag gctggtgact gcggtcagcg cgtcatggga
    21361 acgacgcatg ccacgtttgg aacgggttgg tttattctgt tgtacggcca tggaccttac
    21421 tcctcaatta cggtttatcc ccgatccaac ggggagcacc aattacttac gctttaagct
    21481 ggctaatacg gcaaatgggt ttggcttttg cacttcttca ggcagttcac caaagaccat
    21541 gtccgcttcg gacacttcac agtgttcaga atcatgcacc ggaactaccg gcaaggcgag
    21601 gatgatttca tcttcaacca ttgcaagcag atcgatttca ccgaattcgt taacctcaat
    21661 cggttcatac gcttccggca gtgcttcagc ctgttcgtct gaacgcacag gactaaaaca
    21721 atacgttgtg tagacctgat gagtaaacgg cttcccgcaa cgctgacact cgagcgttac
    21781 cgtcaccttc gcatcgccgt ttaacactgc gagacgttgg ttatcgatag cgaacgacat
    21841 ggagcattcc acatcactgt ccacactgac tacggattcg gcgacgcgct caacctgatc
    21901 aggggtatag ataccctggt aatcaaggcg tttttgagcc gtacgaaccg gatcgagagt
    21961 caggggtaat tttacctttt gcatagggcg cgcatattaa ctttgtaacg tcatagagtc
    22021 aaagaaaaag gcagcctgtg gttgcctttt gccaataatt cgcacacatt gcgggttact
    22081 gctttatttt cgttcagtgc tgataacgaa catcaacctg caaaacgaag cgcatagttt
    22141 aaaatggcta tcatcaatac gctatatctg atggaaaaaa atatgcctaa acttatttta
    22201 gcctccacct cgccatggcg tcgcgctctt ctggaaaaac tgcaaatctc ttttgaatgt
    22261 gcagcaccag aggtcgacga aaccccacgc agcgacgaat caccgcgaca gttggtgctt
    22321 cgactggcac aagaaaaagc gcaatctctg gcgtcacgtt atccggatca tttaattatt
    22381 ggttcagacc aggtatgtgt tcttgatggc gaaatcaccg gtaaaccgtt aacggaagaa
    22441 aatgcccgtc tgcaattacg caaagccagc ggcaatatcg tcaccttcta tactggactg
    22501 gcgttgttta attcggcgaa tgggcatcta caaacagaag tggagccttt tgacgtccat
    22561 ttccgtcacc taagcgaggc ggagattgat aattacgtgc gtaaggagca tcccctgcac
    22621 tgcgcgggta gctttaagag tgaaggattt ggcattacgc tgtttgagcg tttagaggga
    22681 cgtgacccta acacgctggt tggtttaccg ctaatcgcgc tgtgtcagat gttacgtcgg
    22741 gaagggaaaa acccgctgat gggatagttg tttgcgccgg atgcggttac gtcgcatccg
    22801 gcaatcggtg caggcggcct gatgcggcgt aaacgcctta tcaggccaac cgcccattat
    22861 caggcttata tgcttgtatt agcgcgcgtt acgcagcttt tgcaaacaac gcttcaaacc
    22921 ttcatccatc ggcgcttcga tacgcatcac ctcaccggtc cccggatggg taaacttcaa
    22981 cgctgcggcg tgcaggaaca gacgatttaa tcccgtgcct gcttcagtga gctgtctgtc
    23041 aaattcacgg tcaccgtagc gatcgtcaaa ggcaatcgga tgacccgcat actgtgtatg
    23101 cacacggatc tgatgagtac gtccggttac cgggctacaa cgcaccaggg tggcaaatgc
    23161 atagcgttct tccactttaa agcgtgtttc cgacggtttg ccttcctgac tgacacgcac
    23221 gatacgttcg ccgctttgca gaatattttt caataacggc gcttgaacgc tcttcacatg
    23281 cgactgccac tgaccgcgca ccagcgccag gtaatctttt tgcattcctt tttcacgtaa
    23341 ttgctcatgc agagaacgca acgccgagcg ttttttcgct accagcaaca cacctgaagt
    23401 gtcccggtcg agacgatgaa ccagttcaag gaaccgcgct tccgggcgca acgcccgcaa
    23461 accttcgata acgccgaagc ttaaaccact gccgccatgt accgccgtac cggaaggttt
    23521 attcagcacc aggatatgat catcttcata taagatgacg tccgccagcg ccgccacctt
    23581 ttgcagatgt ggcgaaaccg cctcttcttc ccgctcagca acgcgaaccg gtgggatacg
    23641 cacctcatca cccgcttcga gtttatattc aggcttaata cgttttttgt tcacccgcac
    23701 ttcaccttta cgcaaaatac ggtaaatcat actttttggt acgcctttca attgggtacg
    23761 caaaaagtta tcgatacgtt gccccgcttc gtcagcggtg atagcaacaa tttttacgga
    23821 tggagtctct gttttcatgg tgggcgattc taattagcca acaggattcg cgccactcat
    23881 ttttctatgc ttatatttag ttttgcacct tattacttca ctacgtgatc actttattga
    23941 tggttattaa accaatcacc agcaagaagt gaaaaaactg tgagtaagcg ggtgataaat
    24001 ggtaaaagtc atcttgctat aacaaggctt gcagtggaat aatgaggccg tttccgtgtc
    24061 catccttgtt aaaacaagaa attttacgga ataacccatt ttgcccgacc gatcatccac
    24121 gcagcaatgg cgtaagacgt attgatcttt caggcagtta gcgggctgcg ggttgcagtc
    24181 cttaccggta gatggaaata tttctggaga gtaataccca gtctgtttct ttgataattg
    24241 cgctgttttt ccgcatgaaa aacgggcaac cgacactctg cgcctctttg agctgacgat
    24301 aaccgtgagg ttggcgacgc gactagacac gaggccatcg gttcacaccc ggaaaggcgt
    24361 tactttgccc gcagcttagt cgtcaatgta agaataatga gtaagttacg atgaaaagaa
    24421 tgttaatcaa cgcaactcag caggaagagt tgcgcgttgc ccttgtagat gggcagcgtc
    24481 tgtatgacct ggatatcgaa agtccagggc acgagcagaa aaaggcaaac atctacaaag
    24541 gtaaaatcac ccgcattgaa ccgagtctgg aagctgcttt tgttgattac ggcgctgaac
    24601 gtcacggttt cctcccacta aaagaaattg cccgcgaata tttccctgct aactacagtg
    24661 ctcatggtcg tcccaacatt aaagatgtgt tgcgtgaagg tcaggaagtc attgttcaga
    24721 tcgataaaga agagcgcggc aacaaaggcg cggcattaac cacctttatc agtctggcgg
    24781 gtagctatct ggttctgatg ccaaataacc cgcgcgcagg tggcatttct cgccgtatcg
    24841 aaggcgatga ccgtaccgaa ttaaaagaag cactggcaag ccttgaactg ccggaaggca
    24901 tggggcttat cgtgcgcacc gctggcgtag gcaaatctgc tgaggcgctg caatgggatt
    24961 taagcttccg cctgaaacac tgggaagcca tcaaaaaagc cgctgaaagc cgcccggctc
    25021 cgttcctgat tcatcaggag agcaacgtaa tcgttcgcgc cttccgcgat tatttacgtc
    25081 aggatatcgg cgaaatcctt atcgataacc cgaaagtcct cgaactggca cgtcagcata
    25141 tcgctgcatt aggtcgcccg gatttcagca gcaaaatcaa actgtacacc ggcgagatcc
    25201 cgctgttcag ccactaccag atcgagtcgc agatcgagtc cgccttccag cgtgaagtcc
    25261 gtctgccatc cggtggttcc attgttatcg acagcaccga agcgttaaca gccatcgaca
    25321 tcaactccgc acgcgcgacc cgcggcggcg atatcgaaga aactgcgttt aacaccaacc
    25381 tcgaagccgc cgatgagatt gctcgtcagc tgcgcctgcg tgacctcggc ggcctgattg
    25441 ttatcgactt catcgacatg acgccagtac gccaccagcg tgcggtagaa aaccgtctgc
    25501 gtgaagcggt gcgtcaggac cgtgcgcgta ttcaaatcag ccatatttct cgctttggcc
    25561 tgctggaaat gtcccgccag cgcctgagcc catcactggg tgaatccagc catcacgtct
    25621 gcccgcgctg ctccggtacc ggtaccgtgc gtgacaacga atcgctgtcg ctctctattc
    25681 tgcgtttgat cgaagaagaa gcgctgaaag agaacaccca ggaagttcac gccattgttc
    25741 ctgtgccaat cgcttcttat ctgctgaatg aaaaacgttc tgcggtgaat gcgattgaaa
    25801 cgcgtcagga cggtgttcgc tgcgtgattg tgccaaacga tcagatggaa accccgcact
    25861 accacgtgtt gcgcgtgcgc aaaggggaag aaacgccgac cttaagctac atgctgccga
    25921 agctgcatga agaagcgatg gcgctgccgt ctgaagaaga gttcgctgaa cgtaagcgtc
    25981 ctgaacaacc tgcactggca acctttgcca tgccggatgt gccgccagcg ccaaccccag
    26041 ctgaacctgc cgcgactgtc gtagccccag caccgaaagc tgcaacggca acaccagcag
    26101 ctcctgcaca acctgggctg ttaagccgct tcttcggcgc actgaaagcg ctgttcagcg
    26161 gtggtgaaga agccaaaccg accgagcaac caacaccgaa agcagaagcg aaaccggaac
    26221 gtcaacagga tcgtcgcaaa cctcgtcaga gcaaccgccg tgaccgtaat gagcgccgcg
    26281 acacccgtag tgaacgtact gaaggcagcg ataatcgcga agaaaaccgt cgtaatcgtc
    26341 gccaggcaca gcagcaaact gccgagacgc gtgagagccg tcagcaggct gaggtaacgg
    26401 aaaaagcacg taccaccgac gagcagcaag cgccgcgtcg tgaacgtagc cgccgccgta
    26461 acgatgataa acgtcaggcg caacaagaag cgaaggcgct gaatgttgaa gagcaatctg
    26521 ttcaggaaac cgaacaggaa gaacgtgtac gtccggttca gccgcgtcgt aaacagcgtc
    26581 agctcaatca gaaagtgcgt tacgagcaaa gcgtagccga agaagcggta gtcgcaccgg
    26641 tggttgaaga aactgccgct gccgaaccaa ttgttcagga agctccagct ccacgcacag
    26701 aactggtgaa agtcccgctg ccagtcgtag cgcaaactgc accagaacag caagaagaga
    26761 acaatgctga taaccgtgac aacggtggca tgccgcgtcg ttctcgccgc tcgcctcgtc
    26821 acctgcgcgt gagtggtcag cgtcgtcgtc gctatcgtga cgagcgttat ccaacccagt
    26881 cgccaatgcc gttgaccgta gcgtgcgcgt ctccggaact ggcctctggc aaagtctgga
    26941 tccgctatcc aattgtacgt ccgcaagatg tacaagttga agagcagcgc gaacaggaag
    27001 aagtacaagt gcagccgatg gtgactgagg tccctgtcgc cgccgctgtc gaacctgttg
    27061 ttagcgcgcc agttgttgaa gaaatggctg aagtcgtaga agcccccgta ccggttgccg
    27121 aaccgcaacc ggaagtggtt gaaacgacgc atcctgaagt gattgccgcc gcggtaactg
    27181 aacagccgca ggtgattacc gagtctgatg ttgccgtagc ccaggaagtc gcagaacacg
    27241 cagaaccggt ggttgaaccg caggaagaga cggcagatat tgaagaagtt gccgaaactg
    27301 ctgaggttgt ggttgctgaa cctgaagttg ttgctcaacc tgccgcgcca gtcgtcgctg
    27361 aagtcgcagc agaagttgaa acggtagccg cggttgaacc agagatcacc gttgagcata
    27421 accacgctac cgcgccaatg acgcgcgctc cggcaccgga atatgttccg gaggcaccgc
    27481 gtcacagtga ctggcagcgc cctacttttg ccttcgaagg taaaggtgcc gcaggtggtc
    27541 atacggcaac gcatcatgcc tctgccgctc ctgcgcgtcc gcaacctgtt gagtaataat
    27601 tagctcaaag taatcaagcc ctggtaactg ccagggcttt tttatttcat gtttgaatca
    27661 acatattaaa attcatcaaa tggcatcctt gctaaccaac aatgcaaaat aggcaaaaaa
    27721 aatgcccgcc ccggcgatcc cagagcgggc agacaaaaca tacccagttt catgatatgt
    27781 tttaaagcga gcttatttgt tgagctggaa gagcgacaat ccctgcatat cggtaaatgc
    27841 tttatacgat gcctgcaatg ccgtttgctg catgatgtaa gatgaaatag ttgcattcca
    27901 gtccacatca accagatcgc tcatctgctg cgtttgccct aaagcgcgat cgctacctaa
    27961 ggaatccagc gactccagtt tgttcagctg cgtgcctaat tccgcgcgaa cggtaaggac
    28021 attattcagc gagtttttca gtccacggtt ggttttatcc aatgccgcag cggcgatttc
    28081 tttatccgct tcgctatccg cgaccggcgt tttcagggct gcgatggcgc tatccagcat
    28141 ggcaaaaaga ttggtttcag aagcgctacc gtctggttcc gctaccgcgt tactggtaat
    28201 actgtcgaaa attttgtcgc ccgtatgtcc tatcaccatc gaacgcgaag catcgacctg
    28261 ttgcttaata ctttctgttc cgccgacata atcaccgtta gcctcactaa acggcgcagt
    28321 ctctgtttta taaccggcaa aaatgtagcg cccattaccg tcagtggtgt tcgccagatt
    28381 cagcaactgg tcacgaagcc cctgaatatc cgtagccagc gaggcccggt catcgtcact
    28441 caaggtgcca ttgctggcgt agacaatttt ttcctgagca ttctggatag cagtggtgac
    28501 ctggctaagt acactctctt ccagtgacac tttttgagtg gcgaaagtac gtgccagcgt
    28561 gtactggctg ttttgcgcct gtgcctggga gagaacaaca gcttgtgatg cagcaatggg
    28621 atcgtctgaa gggttaacga ctcgcttacc cgtcgatatc tactcgccgt acttcatcca
    28681 ttctgcctga gaattggtga tgccgcgcat gttttgctgg tacatcatct gggtactgaa
    28741 acgcatttta tctcccctta gcgaatgtta atcagagcat caaaaatcgt gtttgccgtc
    28801 tgcagaacct gcgcattcgc caggtaatac tgctgaaaac gttgcagatt tccgtactcc
    28861 tcatcgagat tgacaccgga aatcgactgc tgctgattgg aaagctgcgt caccacatta
    28921 ccttgcgtgg tgctactggt tttcaacatc gcggttttat taccgatatc actcactaac
    28981 gaggcataag cgtcgttaaa ggatttcgca ccgcccaccg ttttactgtt gctttgcaga
    29041 tccagcaggg cctgaccgtt gcggttgtcg ctatcacccg catcttcttc gctcgccatc
    29101 gcgattttcg cttcgtcggt gattaataca tccatgttga cgatggcgtc acttactggt
    29161 ttcagcgtga agctgtcgtt aacggcaggc gttcctgtaa acgtcaactc cagaccatca
    29221 aatgccactt taccgttggc atctggtgtc gccgtaaaag tggtattgct ggcaaggcgg
    29281 gtgacctgcc actgattatt atcgaacgag attttgtaat ctgtcgccag taccacggag
    29341 gcatcagtta ccgtggcacc gatcgcaacg tcacctttgt ttttcgtgtt ttgcagaacc
    29401 gcgggcttac cgatagtaaa gaaatcttca ccagcatcgc cgttagcatc aaatccggct
    29461 ttgtgttggc tgttgaaagc ctcggcaaat gccagcgcca gttgtccaag cgtattacgc
    29521 gtctggtcca gatcctgaga acggaatgtc agaatgccgc ccagcgaccc ggtattcagt
    29581 aatttctccg ggatctcaat attgcctgcc gtcccatcaa cgtaagcgac agtcgtacga
    29641 gaagggtcgg cgctggaagg aactgccgcc agttgccgcg ccgtacttcc ctgaaccagt
    29701 gagtaaccat tggcatcgtg atgttataag taccgccatc ctgaacgctg acttctacgc
    29761 caacaatctg gtttaattcg ctcaccagtt gatcgcgttg atccagcaga ttgttaggtg
    29821 acgcccctgc ccccacgcct gtcaggcgcg agatttgatc gttcaggctg gcaatttgtt
    29881 tagcgtaatt gttgatctga tcaacgctgg cacctatcgc gatattgacc tgtttgtcct
    29941 ggtcgcgcag atattgatcg gtggttttaa actgattcac caacccttct gatttcccaa
    30001 tcagcgcctg gcgcgccgcc gggtcttccg cgttactcac cagcgtttgc aggctggtga
    30061 agaaatcctg catctgtgtt gccagcgaag aggtactggt ggagagcata ttgtcgattt
    30121 tcgacatctg ctcatagcgg gcggtcaggc cgctactttg cgtctgcgca gcacgtaact
    30181 ggttggtaat aaacgtatca tactcacgct gcacaccaga aacgtagacg ccattgccaa
    30241 cccagccgcc agcgcccaac gtgctattgg cctgcgccat aatagtggtt tggcgggtat
    30301 atccggcaac gttatagctg gagatattat tacttgccgt atttaacgcc gcctgggccg
    30361 cgttcagtcc gctcatggcg ttattgatca agctggacat ggaggttcct tataagcctt
    30421 caattacaga gtattatcgg cagcgacccg ccggacttga gttattcaga acagattatc
    30481 aatgttcatg ctgtaggttt tgctcacctt gtcgcttatc gatttcatct gctggatcat
    30541 gttggtgagt ttacgggcat agtgaggatc ggtagcatag cccgcgtcct gtaggacctg
    30601 cgccccctgt tccgcactcg cggcggtcgt cacggcggcg tagcgcgggt tacgtgttaa
    30661 cagcccaacg taatccgaca atgcttccag atacgagctg tagacccgaa acttcgcttt
    30721 tactttcttc gcttcgccgt tttcatattc agtcgtggtg atttcagtga cttgcccttt
    30781 ccagttgcca gaggctttga caccaaacag gttatagctt ggctcgccgt tttcgcggcg
    30841 gatttgccgt tgcccccaac cagattcaag cgccgcctga gcgaggatca aatgatgtgg
    30901 cacaccgctt tgctggcttg ccagttgggc gggcagcgag agttgcgcga ggaatgctct
    30961 actgtcaccc ggcagcgaat catcgtagtt acgtggtacg gccttttgca ccagctgcga
    31021 aagcgtctgg ttttgataac gcaccacggt ttcgagcggg aatttcatcg gtgctgctgg
    31081 cgtggactcc tctggcaatg gttgttctgg cgtcatctgt ttaaccatca tctctgcaag
    31141 ccccagacct ttgcccgccg tcatctgttg ggcaatttgc tggtcataca tactggtata
    31201 cagacgagtg tgctcgctgc tgaacaggcc atcttttggt aaagcgtcgc gcatgctttt
    31261 caacatcatc tgcacgaaca tcccttccac ctgacgggcc accggacgga tatttgccgc
    31321 cggatcttcg ctcgatttcg cctttagttc gttgagtgat tgcgcatccc aggccgcact
    31381 tgccagtagt ttgctgtcgc tgatcatcag atgatttcca gttttgcccg cagacatccc
    31441 gcactttgca ttgattgcaa aatagacatc agatccatcg gcgtagcgcc cagcgcattg
    31501 agcgcacgca ccacgttatt gaggctggcg ctggaacgta cgctttgcag cgaaccgccg
    31561 ctctggcgta aatcgatctg cgtttgtgga gtaacaacag tctgtccacc accaaacggt
    31621 gtatctggct ggctgacatt ggcctgacga ttaactgtta ctgagagatt tccctgcgct
    31681 accgcgcagc tgtcgagggt cacttcgcga ttcatcacca ccgaaccggt gcgcgagtta
    31741 atcactactt tagcgtcctg cggggtgaca ttaacctgca tattctggat atcggcaagg
    31801 aaacggacct gggaactgtt gccacttggt acgcgcacct gaatagtccg cgcatctaac
    31861 gcggtggcgc tgccatatcc acgcacgcgg ttgatggtgt cagcgatttg ctgcgccatg
    31921 ctgaaatctt cgtcgttaag ttgcaaatta agggtattcc cgacgccaaa ctggctgggc
    31981 aattcacgtt caataaccgc accattggtg atccgtccac cgttcagttg gttaacctga
    32041 acactgctac cgcccgcgga ggctcctgcg ccgccaacca gaatattgcc ctgcgccagc
    32101 gcatacacct gactgtcaac gcccttaagc ggtgtcatca acaacgtacc tccacgcaag
    32161 cttttggcat tacccatgga agaaaccacc acatcgatgg tttgcccctg acgtccaaac
    32221 ggaggaagtg acgctgtcac cattaccgca gcgacgtttt ttagctgcat attggtgccc
    32281 gtcggaacgg taattcccag ctgtgagagc atgttattaa gcgtttgtgt ggtaaacggc
    32341 gtctgggttg tctggtcacc ggtgccatcc agccccacta ccaggccata gccaatcagt
    32401 gagttttgcc ttaccccctg aacactggtg agatcgcgaa tacgctcagc ctgagccgcc
    32461 gtcgtaacca gtagaagaat taatgcagag agaaatttaa tcaccacagc ctcacttaca
    32521 ttggcgacag gttaaggaag aaacgctgca accagcccat attttgcgct tcgttaatgt
    32581 agccattgcc tacgtattca atacgcgcat ccgccacctg agtagacggt acggtattgc
    32641 tgccgctgat agtgcgtgga ttaaccacgc ccgagaagcg aataaattcg gtaccctgat
    32701 taatggcaat ctgtttttca cccaccacat gcaggttgcc gttgaccagt acctggtcaa
    32761 ccgtcaccgt caacgtgccg ctaaaggtat tgctggcatt ggccccgccc tttccgttga
    32821 acgtgttacc accggaggct tcgacatcgg cacgagcgtt accaaacagc ccctgcaaat
    32881 agcgcggcac agtatcaaag ccaaaattag ttttaccgtc acggctgaca ttcgcagagg
    32941 agcttttgct ggcgctgacg ttctcctgca acacgatggt cagcgtatcg ccaatattgc
    33001 gtggtcgacg atcttcaaac agcggttgat agccatagtt aatcggctga gcagactgga
    33061 aaatagaacc gttggcgacg ggcgtcggac cgggaaccgg ttgtgcactg gtcgccccct
    33121 gcaccagcgg cgtggagggt atccaggcgc agccggttag tgaaagcacc aacaagctgg
    33181 aaatggcata agtatgcgca gcgttttttt gcatggctat catcttcaaa aatcagtaaa
    33241 ccggtgaacc tgccaccggt tatgccttag agttgcgtca gtttttgcag catctgatcg
    33301 gtggtggaca ccgctttact gttgatttcg taagcgcgtt gcacctgaat catattgacc
    33361 agttcttccg ccacgttgac gttagacgtt tcaacatacc cttgatacag cagtcccgcg
    33421 ccgttcaggc ccggcgtgct ttcgttcggt gcaccggagg attgcgtttc ggtgtagagg
    33481 ttttcgccaa tgctctccag tccggtatca ttcataaagg tggtgagatt gagctgccca
    33541 acctgaaccg gagctgcctg gccttgttgg gttacgctga ccacgccatc acgaccgatg
    33601 gtgatactta acgcattcgc cggaatggtg atcgctggct gcacctgaaa accaccagcc
    33661 gtcaccagct gcccgttctg atccacctgg aaagagccgt cacgggtata ggctgatgag
    33721 ccatctggca acattacctg gaaaaagccc tgcactttaa tcgcgacatc tttgctgtta
    33781 ttggtctgcg acagattgcc ctggctgtgt aagcgttcag tggcgaccgg gcgtaccccc
    33841 gtgccgattt gtaatccgga gggtaaggtg gtttgttcgg aagactgtgc ccccggctgg
    33901 cgaatggttt gataaagcag atcttcaaac accgcgcgct gacgcttaaa accgttagta
    33961 ctgacgtttg ccaggttgtt ggcaatgacg tccatattgg tttgctgggc gtcaaggccc
    34021 gttttggcga tccataatga actgatcata taatgtcctg tagtggattg ctgacaagca
    34081 gagcagaagc acgcgacagt gtgaatttcc cctcacccta accctctccc caaaggggcg
    34141 agggaatagt ccgcgcgctt ttattgcaat gtcatcaatc gttcaattaa ctcatcgaca
    34201 gcagttggtt ggcacggcct gcgttatcat cgacgctgct gatcaccttc atctgcattt
    34261 caaaacgccg cgcgctggca atcatgtcgc tcattgccgc aacggcattg acgttactgc
    34321 cttccagaac ccccgacatc acacgcaagg ttggatctgc ctgcagtacc ggccctcgcg
    34381 tggcctgggt ttctgcgctt aaacgaaaaa tgccgtcgtc accgcgctgc acttcgctgc
    34441 ccgtggcttt catcagtttc agacgcccta ctggcgcaac tgtatttgcc ggatcgcccg
    34501 gattgagcgc cgagattgtg ccatcggcag caatcgtgat ttccgcccct tccggcacag
    34561 caattggccc cgcctcgcct atcaccggat gcccctgaat tgtcagttgc ccggtgggat
    34621 caacctgaat gctgccatta cgcgtatacc cttcgctgcc gtcagcggtc tgcacggcca
    34681 gccatcctgc tgcaacgcaa cgtccagcgg gcacgaggtg taatccatct tgccgggcgt
    34741 catgtctgca cccggtgttg acgccgttac cagcgtgcgc gtgggcagtg aaagcccttc
    34801 caccggcact gcgcgcaagg cgtttaactg cgcgcgaaaa ccgggcgtag aggcattggc
    34861 cagattactg gcggttaccg cctgttgatt cagcgtctgg ctggctgctc ccatcgcagt
    34921 ataaattgcg tgatccattg agctatcccg tcagcgatta gcgtaagtta accagcgtgt
    34981 tgaggatctg gtcctgggtt ttgatggtct gggcgttaga ctgatagtta cgctgggcaa
    35041 cgatcatatt gaccagttct ttactgagat cgacgttgga cgcttccagc gcaccgttgg
    35101 tcagggtgcc aaagtttccc gtcccggctg tccccaacag cgccacgcca gaagattgcg
    35161 tcgcagacca gacgttgtcg ccttcggatg ccagaccttc gttgttggca aagttcgcca
    35221 gtacaatccg ccccagcagt tgggtttgtt cgttggaata gttgccgaca accgtaccgt
    35281 catcattgat ttgataactc accagatcgc ccggtttgta gccattctgg gtggttgcca
    35341 caatgttgtt agcgccggta ttttgctgca tggagttgag gaagctcaga ctaaacgtgg
    35401 cgggttctgc accgttaatt gcgccggttg cgatattatt cgccatcgca ccatccacta
    35461 atgtgccatt agcattaaat tccagtgttg tcgctgtctt cgcaatgctg tttggatcac
    35521 tgctatcctg ggtgtagacc tgccagttat tatccccggt cttcacaaaa tagacgctca
    35581 tgtcatgagc attaccctga ctgtcgaaaa cagtcaccga accttttttg ttatagctat
    35641 ccgcattgct ggcgctgaat ggcgtaacag taggaagcgg atcactggaa ttcaggttga
    35701 tctgcatcga cgccgtggtg gtagttttcg ctgccatcag ggtattcggg atcgaaatat
    35761 tggtcggatt cgccccttgc tgaatagtcg gcggcgtacc ggttgccggg taacccgtca
    35821 gctgtaaacc ttgcatattc accaggttac ggttttcatc cagcttaaat tgtccgttac
    35881 ggctgtagaa caccgaaccg ttgctgtcta ccagacggaa aaaaccgttc tggctgatag
    35941 caacgtccag acctcgcccg gtgttggtgg tcgtgccatc ggtaaagtcc tgagtgatac
    36001 cggcaacttt tacccccagt cccactttcg aaccggcaaa catatcggca aaagaggccg
    36061 tgcctgattt aaagccgtag gtggcggagt tggcgatatt gttgccaata acatcgaggt
    36121 tggtggcggc agcgtttaat ccgctaaccg cttgagaaaa ggccatgact gactcctgaa
    36181 atgtgaaggc ttaaattatc tgccgtactt cgtcgagggt ggtggtgccg taagtgccga
    36241 gatccagcgt attaccgctg ttgccgcgga tcacaccctg caccagagca aactgcagcg
    36301 gctgggcaac cagttgtgta ccaccgttac tggcgctaat cgctacattg taagaaccgt
    36361 tcggcgcagt gctgccatca gtcaacgtac cgtcccaggt gaaactggga actccggcgg
    36421 tcagttcacc aatatcaatg gtgcgcacaa ccgcgccatt tttatcggtg atggtggccg
    36481 taactttgtc tgccgcctgt tgcagctcaa caccaaacgg cgtggtcgtg gtcacagccc
    36541 cttcttcact gccggttccc gcaagaacag ttgtgccggg gatcatcacg ccgtgaccga
    36601 tcaggttact ggcctgtaac gactggctgt tatcaatctg tccggaaata gatccgagcg
    36661 tggtattgag tttttcaatc ccgctgaccg tgctgatttg tgccaattgc gacgtcagct
    36721 cgttgttttc cattggattg gtcgggtcct ggtttttcag ctgcgccacc agcaaagtca
    36781 gaaaactgct ttgtaaatct gcggcgttgc tgcccgtgag cgaactactg ctggtggtac
    36841 tgacgccggt atttgtcgga tcggtggtgg ttaccgcaat ggacatagct ttctccttta
    36901 ttgaccgagc gtaagggttt tcagcaccat gcttttcacc gtgttgagca cttcaacatt
    36961 ggcctgatag ctgcgtggcg ccgacatggt gttaaccatc tccccgacga catcaacgtt
    37021 cggcattttt acgtagccct ttgcatctgc cagcggatta cctggttcat aaaccagttt
    37081 gtccggggcc tgactttcta taacatcggc aacctttacg ccgcctgtcg cagcacctgg
    37141 tgcagcgtta acctggaata ccacctgttt tgcccgatat ggctgtccat caggaccggt
    37201 cacgctatca gcatttgcca gattactggc cgccacgttc aggcgctggg actgggcagt
    37261 taacgccgac ccggcgatat caaaaatatt cagcagtgcc atccgttaat ttccgctctg
    37321 taaaacgttc atcatgcctt tgatttgccc gctcaacgcg ctaaggctca tctggtattg
    37381 caggctgtta tcggcaaact gggtgcgttc gcgatccata tcgacggtat taccgtcaag
    37441 cgaaggctgg tccggaatac ggtattgcag ttctgcggta ggaggcgtca gcgcctgcgc
    37501 cgggatgtgt tgcgttgagg tcatcgtcag tgcaaccaca ctggttgcat cccgtccacg
    37561 ttgcatgact tttttaagtt cactggcaaa atcgatatcg cgcgcctgat aaccaggggt
    37621 atcggcattg gcgatgtttg ctgccagcac ttcctgacgc tgggcgcgca gattgagcgc
    37681 ctcttgttga aaacgtaagg cggcgtcgag cttatcgagc atatctcctc cgcaggtatc
    37741 aaaattctgc catcagctta aatgccttta cgcgcgcgtt atcggcggaa taaacgcaaa
    37801 atgggtcgct atttatgccg ttgatggtca ttgcggacag gtacaattca cgttgtagaa
    37861 atggctgggg gcgaaaatgc tgacaataaa acgtagcgtg gcgatcatcg cgatactgtt
    37921 cagtccgtta agtacggcga gcaatctcac atcgcaattg cacaactttt ttagcgccca
    37981 actcgtgggg gtaagtgatg aggttcgtgt ttctattcgt acagcgccca atctactacc
    38041 gccatgcgag cagccattgc tttcgatgag caataattcc cgcctgtggg gcaatgtgaa
    38101 tgtgttggca cgctgcggta acgacaaacg atatttacag gttaatgtgc aggccacagg
    38161 aaattatgtg gttgccgcga tgcctattgt gcggggagga aagctggaag ctggcaatgt
    38221 caaactgaaa cgtggacggc tggataccct gccaccgcgt acggtgctgg atatcaatca
    38281 acttgttgat gccgttagcc tgcgcgatct atcacccgat caacctatcc agttaaccca
    38341 gtttcgccag gcatggcggg taaaagcggg acaacgcgtc aatgtgatcg ccagcggtga
    38401 tgggtttagc gccaacgcag aaggtcaggc gctgaacaat gcagccgtcg cacagaatgc
    38461 gcgggtgcgc atggtatcgg gacaggtagt cagcggcgtt gttgatgcag atgggaatat
    38521 tcttataaac ctgtaagctg ttaaagatta cccgtccctt gccgataaat aagcaacaca
    38581 tgataaaagc gccctcaatg aggaataaac catgagtatt gatcgcactt cgcctctgaa
    38641 gcctgtaagc accgttcaac cgcgcgaaac cactgacgcg ccggtaacga acagccgggc
    38701 ggcaaaaaca accgcctcca ccagcaccag tgtgacgtta agcgacgcgc aagcaaaact
    38761 gatgcaaccc ggcagcagtg atatcaatct tgaacgtgtc gaagcgttaa aactggcgat
    38821 tcgtaacggt gaactaaaaa tggacaccgg caaaattgcc gatgcgctga tcaacgaagc
    38881 gcagcaagac ttgcagagta actgatcgta tgacacgtct tgcagagatc ctcgaccaga
    38941 tgtccgctgt gcttaacgat ctcaaaacgg taatggatca agagcagcaa catctctcta
    39001 tgaggcagat caacggcagc cagttgcaat ggattacaga acaaaaaagc tcactgctgg
    39061 cgacgctgga ttacctcgaa cagttacgca agaaagaacc caatacagca aatagcgttg
    39121 atattagtca acgctggcag gaaattactg tgaaaacgca gcaactacgc caaatgaatc
    39181 aacataacgg ctggttactg gaaggacaga ttgagcgcaa tcaacaggcg ctggaaatgt
    39241 tgaaaccgca tcaggaaccg acgctatatg gggcgaacgg tcagacctca acaccccatc
    39301 gcggcagtaa aaagatttcg atctgaatat tatctccggc ctgcactgca ggccggaatg
    39361 cattgttaca ccgtccggcg ggcaaattct ttaactttga agcccagtac cgccagtgca
    39421 gcgaagtacg cggcaatccc cgccagcacg accgccatta aacgcagtaa gcgccagggc
    39481 atggtaccca atgaccactc cggcatgata tgtaacatac ctaaaagcac gccagacatc
    39541 accagtaccg ccaccaccag acgcaacaga aacgccatcc agccgggttg cggagtaaag
    39601 attttctgct tacgcaactg ccagtaaagc agcgaagcat tcagacacgc cgccagacca
    39661 atagaaagtg acagcccggc atgtttcaac ggaccaataa acgccaggtt catcaattgc
    39721 gttaaaatca gcgtaacgat ggcaattttc actggcgttt taatgtcctg gcgggaataa
    39781 aagccaggag ccaacacttt cactacaatc aggccaatca aacccaccga gtaggcaatt
    39841 aacgcccgct gggtcatcag cgcatcaaac gcggtaaatt taccgtactg gaacagcgaa
    39901 acggttaacg gacctgaaag aatgcccaac gcaaccgcac tcggcagtgc caacaggaaa
    39961 caaagacgca acccccagtc catcaaacgg ttgtattcat catgattgcc actggcaaaa
    40021 cttttcgaca gcgacggcag caaaatggtg ccaagcgcca cgcccagcac accggaggga
    40081 aactccatta agcggtcggc gtaatacatc caagacaccg aaccggaagc aagaaacgag
    40141 gcaaaaatgg tgttgatgat taaggagatc tggctcacag agacgccaag gatcgccggc
    40201 cccatctgtt tcaccacgcg cattgctccg gcatcgtgga agttaatgcg cggcaggacc
    40261 agcatgccga tcttcttcag gtgcggtagc tgatacacca gctgcaggat gccgccgacc
    40321 gtaacagccc acgccagcgc cagcaccggt gggttaaagt acggtgcggc aaacagcgcg
    40381 aaaccaatca tgctgatgtt aagcagtgtt ggcgcaaacg ccggaatcga gaagcggttc
    40441 cacgtattca gaatcgctcc caccagcgac gccagggaga tcagcaagat atagggaaag
    40501 gtaatcttta gtagctggct ggtcagggca aatttgtcag ctgtgtcagc gaagcctggc
    40561 gcggtcacca tgatcaccca cggtgcggcg agcatgccag cgaccgtcac aaccgccagc
    40621 gcaagtgtca gcaggccaga aacataagag acaaagaccc gcgtggcgtc ttcaccctgc
    40681 ttacttttat attccgccag aatcggtaca aatgcctggg aaaatgcccc ttcggcaaag
    40741 atacggcgta acaagttagg aagtttaaaa gcgacgaaaa aggcgtcggt tgccatccct
    40801 gcgccaaaga ttctggcgac aattgcgtct cgtgcgaagc caagcacacg cgaaaacatg
    40861 gtcatcgagc tgacggcggc cagcgatttt aataaattca tcggtgttct aatccggacc
    40921 cacgggcaaa acgcctgcaa ggcaggcgct gaaagaggcg aatagtctac ctggattatg
    40981 gtgaattgct accgccagat gttacagggt ttattcactc atcgcatcgc gccagatctt
    41041 gtcaacgata cgttgcgcca gcactgcctg ttcgccggcg gtttgcggaa ctgtctgatt
    41101 ttgcacgcat tcaatgaagt gccgcgcaca gccgacaaat ccacgttgct caagagtgct
    41161 ctgccaacca ggaatcggtt tattcactac accctgcccg cgctcctcac gccattcgcg
    41221 catatccgta atgtcgatga gcgcaccgtc agtcacggcc tgcacggttt cacgctgact
    41281 tccggcacgg cgatgcatac aagtggtgat ctgcaaagga ccggccgaaa aatggtgctc
    41341 ggcaaacagc atttcgccag cgtcgttagt cagtagcgta ccgccatcca gagaggcttt
    41401 gccgcccgac aaccacagcg cggtatccac cacatgcaga taatcatcca gcaacgtgaa
    41461 ataaagatcg tgtggcccga cgctattgct acgatgttta tccattctta gcgaggctgc
    41521 ggtggcgagt tgcgttttta actcaccgta gagtggtgcg aaacgacggt taaaaccgac
    41581 catcaacgtc agttttttcc gcgccgccag ttccaccagc ctttcagcat cgcgcagatt
    41641 ttctgccagc ggtttatcga cacagacatg tacccccgca ttgagtaacg tactgaccac
    41701 gtcaaagtgg ctggcggtgc tggagtgcac aaaaaccgca tcacaactgg cggcaaggct
    41761 ggataacgaa tcggcataag gaatgcgcca gctgtcacaa attggcaggg ctttggcgcg
    41821 cgtaggcgac caggctcctt gtaacgtcca gtcagacgct gccgccagca ccggtaacca
    41881 cgctttttgc gcaatgccgc ctaatcccac tacgccgata cgtaattttt tcactttaat
    41941 ctcccagatg ggccagcaac gaatcaagac gttgtttcag ttctgccact tcgatttcca
    42001 gggcttcgac gcgggtctgt aaatcaccgt caaccacgtt cgacatagcc gttaccgccg
    42061 gctgatcttc aacctcacca ctgaaaagat gcatgtagcg gctttcgcgt ttacccggtt
    42121 cgcgggccag acgcaccaca aaaggaccat cttcgcgatt cgccagttgt tccagcgtcg
    42181 actccacttc cgccatatcg ctgaattcat acattcgcgc ggcgcggctg cgcagttcac
    42241 ccggcgtctg ggcaccacgc aataacaacg tggtgattaa cgccacttct gcggcgctca
    42301 gtttcagatc gccaaattct gaattacaaa aacgttgctc atatttggtg acccgattac
    42361 caaaaccgct cactgtgcgt agataatgac gtttgaccag attatccagc tgttcctgca
    42421 cttcggattc actcagattc atgaccggtt cacggttcgt tttctgatta caggccgtga
    42481 ctacaccatt gactgagagc gggtattgct ccggcgtggt cacctgcttt tccagcaagc
    42541 aaccaattac gcgagcttcc agggcagtaa gttgatattt catgagattc tccttagcgg
    42601 ccgggcgtcc agtctggggt agttaatgcc gtcagtacgt gatcgcgcca ttgtccatca
    42661 atcaacagat agtctttcgc atagccttct ttttcaaaac ccagtcgcgc cagtaaatca
    42721 ccgctgcgtt tattgtgcgg catataatta gccataatgc gatgaatatg ttgggtgcgc
    42781 tgcatataac gaatggctgc ggtcagggct tcaaacatga gtcctttgcc ctgccatttt
    42841 tgcccaatcg aataaccgag atagcaggca tgaaaagagc cgcgaacaac attggaaaaa
    42901 ttggcaacgc caataatctc tttttcgtcc gggtcgaata agccaaagta gaaagctgaa
    42961 ccttgtttat gaaattcgtt aatcatccct agcctggcct gccagcctga tggataacag
    43021 tggctttcgt cgcgcactgg ctcccagggc ttgaggaaat ggcgattctc tgcgtaataa
    43081 tccgccagac gccaggcatc acgatcatgc accagacgca cgaccagtcg gtctgtggtt
    43141 aagcgcactt ttggcacgtt actgcgatag ccaaacattc tatacctact ccttcccgta
    43201 acgtctttaa tacgctgtaa gtattactat acctgcgcaa ccagaccctg tgaaaagtcc
    43261 aaaatgccat tttctgcatt attcacatat ttgataaaaa ctctctatca aagcgacgtt
    43321 ttgataaaaa aatattgtcc ccgaagtgat gggaaaagtc ttagcggcaa tgcagaatag
    43381 aaagtgctca tcttttattt cccgggaggg gaaatgtccc gcgtatcgca ggcgaggaac
    43441 ctgggtaaat atttcctgct catcgataat atgctggtcg tgctggggtt ctttgttgtc
    43501 ttcccgctga tctctatccg cttcgttgat caaatgggct gggccgccgt catggtcggt
    43561 attgctctcg gtctacgcca gtttattcag caaggtctgg gtattttcgg cggcgcaatt
    43621 gccgaccgct ttggtgccaa accgatgatt gttaccggta tgctgatgcg cgccgccgga
    43681 ttcgccacaa tgggtatcgc ccacgaaccg tggctattgt ggttttcatg cctgctctcg
    43741 ggactcggcg gcacgttgtt tgatccgccg cgttcggcgc tggtggtgaa actaatccgt
    43801 ccacaacaac gtggtcgttt tttctcgctg ttgatgatgc aggacagtgc cggtgcggtc
    43861 attggcgcat tgttggggag ctggctgttg caatacgact ttcgcctggt ctgcgccaca
    43921 ggggccgttt tgtttgtgct atgtgcggcg ttcaatgcgt ggttgttacc ggcatggaaa
    43981 ctctccaccg tacgcacgcc cgttcgcgaa ggcatgaccc gcgtgatgcg tgacaagcgt
    44041 tttgtcacct atgtcctgac gctggcgggt tactacatgc tggctgtaca agtgatgctg
    44101 atgctgccaa ttatggtcaa cgacgtggct ggcgcgccct ctgccgttaa atggatgtat
    44161 gccattgaag cgtgtctgtc gttaacgttg ctctacccta tcgcccgctg gagtgaaaag
    44221 cattttcgtc tggaacaccg gttgatggct gggctgttga taatgtcatt aagcatgatg
    44281 ccggtgggca tggtcagcgg cctgcaacaa cttttcaccc tgatttgtct gttttatatc
    44341 gggtcgatca ttgccgagcc tgcgcgtgaa accttaagtg cttcgctggc ggacgcaaga
    44401 gctcgcggca gctatatggg gtttagccgt ctgggtctgg cgattggcgg cgctattggt
    44461 tatatcggtg gtggctggct gtttgacctg ggcaaatcgg cgcaccagcc agagcttccg
    44521 tggatgatgc tgggaattat tggcatcttc actttccttg cgctgggttg gcagtttagt
    44581 cagaaacgcg ccgcgcgtcg tttgctggaa cgcgacgcct gatttgatac tcccccctga
    44641 ctcctccata ctgaaagtga actgacagcc aaatggagga gtcacgtgaa gctatacatt
    44701 tacgatcact gcccttactg cctcaaagcc cgcatgattt tcggcctgaa aaatatcccc
    44761 gtcgaattac atgttctgct caacgacgac gcagaaacac ccacccggat ggtcggtcaa
    44821 aaacaggttc ccattctgca aaaagatgac agccgctata tgccagaaag catggatatc
    44881 gttcactatg tcgataaact cgacggcaaa ccgttactga ccggcaaacg ttcccctgcc
    44941 attgaagagt ggctgcgcaa ggtcaatggc tacgccaaca aactgctgtt gccgcgtttt
    45001 gccaaatcgg catttgatga gttttctact cccgccgcgc gcaaatattt cgtcgacaag
    45061 aaagaggcca gcgcgggtaa ttttgccgac ctgctggccc actctgacgg tctgattaag
    45121 aatatcagcg atgatttgcg tgcgctggac aaactgatcg tcaaaccgaa cgccgtgaat
    45181 ggcgaacttt cggaagatga tattcagcta tttccgctac tgcgtaatct gacgctggta
    45241 gccggaatca actggccaag ccgcgttgct gattaccgcg ataatatggc gaaacagaca
    45301 caaatcaatt tgttatcatc aatggcgatt taatcctgac ccgccgcggc agcctgtatt
    45361 cgcgggttgc cgccgttttc agttttttct gctggcgagt ttatcgctgg tgacacatgc
    45421 tgcgttgtgt caggatattg acgcagattt catgtcgagg actaccatga acaagttttt
    45481 atttgccgct gcattgatcg tcagtggcct gctcgttggc tgtaatcaac tcacccaata
    45541 caccatcacc gaacaagaaa ttaaccagtc gcttgcgaaa cataataatt tctcaaaaga
    45601 tatcggttta cccggcgtgg ctgacgccca tattgttctg acaaacttga ccagccaaat
    45661 tggtcgcgaa gagccgaata aggttaccct aaccggagac gccaatctgg acatgaactc
    45721 cctgttcggt agtcagaaag cgaccatgaa actgaagctg aaagcgctgc ccgtgtttga
    45781 taaagaaaaa ggtgcgatct tcctgaaaga gatggaagtg gtcgatgcga cggtacaacc
    45841 ggaaaaaatg caaacggtga tgcaaacgtt gcttccctat ttgaaccagg cattacgcaa
    45901 ttactttaac cagcaacctg cttacgtcct gcgcgaagat ggcagtcagg gcgaagcaat
    45961 ggcgaaaaaa ctggcgaaag gcattgaagt gaagccaggc gaaattgtca ttccgtttac
    46021 ggattaatga cgagggcgca ttcgcgccct ttatttttcg tgcaaaggaa aacgtttccg
    46081 cttatccttt gtgtccggca aaaacatccc ttcagccgga gcatagagat taatgactgc
    46141 accatcccag gtattaaaga tccgccgccc agacgactgg caccttcacc tccgcgatgg
    46201 cgacatgtta aaaactgtcg tgccgtatac cagcgaaatt tatggacggg ctatcgtaat
    46261 gcccaatctg gctccgcccg tgaccacagt tgaggctgcc gtggcgtatc gccaacgtat
    46321 tcttgacgcc gtacctgccg ggcacgattt caccccattg atgacctgtt atttaacaga
    46381 ttcgctggat cctaatgagc tggagcgcgg atttaatgaa ggcgtgttca ccgctgcaaa
    46441 actttacccg gcaaacgcaa ccactaactc cagccacggc gtgacgtcaa ttgacgcaat
    46501 catgccggta cttgagcgca tggaaaaaat cggtatgccg ctactggtgc atggtgaagt
    46561 gacacatgca gatatcgaca tttttgatcg tgaagcgcgc tttatagaaa gcgtgatgga
    46621 acctctgcgc cagcgcctga ctgcgctgaa agtcgttttt gagcacatca ccaccaaaga
    46681 tgctgccgac tatgtccgtg acggaaatga acggctggct gccaccatca ctccgcagca
    46741 tctgatgttt aaccgcaacc atatgctggt tggtggcgtg cgtccgcacc tgtattgttt
    46801 acccatcctc aaacgcaata ttcaccaaca ggcattgcgt gaactggtcg ccagcggttt
    46861 taatcgagta ttcctcggta cggattctgc gccacatgca cgtcatcgca aagagagcag
    46921 ttgcggctgc gcgggctgct tcaacgcccc aaccgcgctg ggcagttacg ctaccgtctt
    46981 tgaagagatg aatgctctgc agcactttga agcattctgt tctgtaaacg gcccgcagtt
    47041 ctatggcttg ccggtcaacg acacattcat cgaactggta cgtgaagagc aacaggttgc
    47101 tgaaagcatc gcactgactg atgacaccct ggtgccattc ctcgccgggg aaacggtacg
    47161 ctggtccgtt aaacaataaa aatcgtagct tcctgttgtc attaggttat tttacctgta
    47221 taaataacca gtatattcaa cagggggcta ttatgcgaat tgaagtcacc atagcgaaaa
    47281 cttctccatt gccagctgg
//
LOCUS       DADLSA010000015        47036 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.015, whole genome shotgun sequence.
ACCESSION   DADLSA010000015 DADLSA010000000
VERSION     DADLSA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.015"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="JN691_000878"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="JN691_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAY7242387.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLCFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1958)
                     /locus_tag="JN691_000879"
     CDS             complement(1614..1958)
                     /locus_tag="JN691_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAY7242388.1"
                     /translation="MFFGGHVLRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAM
                     LSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGTSKELWDSRPEGSGWSWKDLAWDVAGA
                     STGYTVWQLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JN691_000880"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JN691_000880"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAY7242389.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JN691_000881"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JN691_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAY7242390.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /locus_tag="JN691_000882"
     CDS             complement(6144..6842)
                     /locus_tag="JN691_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAY7242391.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JN691_000883"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JN691_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAY7242392.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="JN691_000884"
     CDS             7537..8574
                     /locus_tag="JN691_000884"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY7242393.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMVANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JN691_000885"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JN691_000885"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAY7242394.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="JN691_000886"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="JN691_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAY7242395.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JN691_000887"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JN691_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAY7242396.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="JN691_000888"
     CDS             10745..11626
                     /locus_tag="JN691_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7242397.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12989)
                     /gene="srmB"
                     /locus_tag="JN691_000889"
     CDS             complement(11661..12989)
                     /gene="srmB"
                     /locus_tag="JN691_000889"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAY7242398.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13121..13858
                     /gene="trmN"
                     /locus_tag="JN691_000890"
     CDS             13121..13858
                     /gene="trmN"
                     /locus_tag="JN691_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAY7242399.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13843..15465)
                     /gene="nadB"
                     /locus_tag="JN691_000891"
     CDS             complement(13843..15465)
                     /gene="nadB"
                     /locus_tag="JN691_000891"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAY7242400.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15721..15876
                     /gene="rseD"
                     /locus_tag="JN691_000892"
     CDS             15721..15876
                     /gene="rseD"
                     /locus_tag="JN691_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAY7242401.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWCFDSAWKFG
                     LGRLYLG"
     gene            15873..16448
                     /gene="rpoE"
                     /locus_tag="JN691_000893"
     CDS             15873..16448
                     /gene="rpoE"
                     /locus_tag="JN691_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAY7242402.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16481..17131
                     /gene="rseA"
                     /locus_tag="JN691_000894"
     CDS             16481..17131
                     /gene="rseA"
                     /locus_tag="JN691_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAY7242403.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17131..18087
                     /gene="rseB"
                     /locus_tag="JN691_000895"
     CDS             17131..18087
                     /gene="rseB"
                     /locus_tag="JN691_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAY7242404.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18084..18563
                     /gene="rseC"
                     /locus_tag="JN691_000896"
     CDS             18084..18563
                     /gene="rseC"
                     /locus_tag="JN691_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAY7242405.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18761..20560
                     /gene="lepA"
                     /locus_tag="JN691_000897"
     CDS             18761..20560
                     /gene="lepA"
                     /locus_tag="JN691_000897"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAY7242406.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20576..21550
                     /gene="lepB"
                     /locus_tag="JN691_000898"
     CDS             20576..21550
                     /gene="lepB"
                     /locus_tag="JN691_000898"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY7242407.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21822..22502
                     /gene="rnc"
                     /locus_tag="JN691_000899"
     CDS             21822..22502
                     /gene="rnc"
                     /locus_tag="JN691_000899"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAY7242408.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22499..23404
                     /gene="era"
                     /locus_tag="JN691_000900"
     CDS             22499..23404
                     /gene="era"
                     /locus_tag="JN691_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAY7242409.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPIILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23416..24144
                     /gene="recO"
                     /locus_tag="JN691_000901"
     CDS             23416..24144
                     /gene="recO"
                     /locus_tag="JN691_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAY7242410.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24156..24887
                     /gene="pdxJ"
                     /locus_tag="JN691_000902"
     CDS             24156..24887
                     /gene="pdxJ"
                     /locus_tag="JN691_000902"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAY7242411.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVDAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24887..25267
                     /locus_tag="JN691_000903"
     CDS             24887..25267
                     /locus_tag="JN691_000903"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAY7242412.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25687..25767
                     /gene="shoB"
                     /locus_tag="JN691_000904"
     CDS             25687..25767
                     /gene="shoB"
                     /locus_tag="JN691_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAY7242413.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFS"
     gene            complement(25961..26221)
                     /gene="yfhL"
                     /locus_tag="JN691_000905"
     CDS             complement(25961..26221)
                     /gene="yfhL"
                     /locus_tag="JN691_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAY7242414.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(26277..27125)
                     /locus_tag="JN691_000906"
     CDS             complement(26277..27125)
                     /locus_tag="JN691_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY7242415.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            27334..27969
                     /gene="yfhb"
                     /locus_tag="JN691_000907"
     CDS             27334..27969
                     /gene="yfhb"
                     /locus_tag="JN691_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAY7242416.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            27994..28530
                     /gene="tadA"
                     /locus_tag="JN691_000908"
     CDS             27994..28530
                     /gene="tadA"
                     /locus_tag="JN691_000908"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAY7242417.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(28527..30083)
                     /gene="mltF"
                     /locus_tag="JN691_000909"
     CDS             complement(28527..30083)
                     /gene="mltF"
                     /locus_tag="JN691_000909"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAY7242418.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYLIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            30341..34228
                     /gene="purL"
                     /locus_tag="JN691_000910"
                     /gene_synonym="purI"
     CDS             30341..34228
                     /gene="purL"
                     /locus_tag="JN691_000910"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAY7242419.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            34804..36231
                     /gene="qseE"
                     /locus_tag="JN691_000911"
     CDS             34804..36231
                     /gene="qseE"
                     /locus_tag="JN691_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAY7242420.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSISFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            36396..37109
                     /gene="qseG"
                     /locus_tag="JN691_000912"
     CDS             36396..37109
                     /gene="qseG"
                     /locus_tag="JN691_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAY7242421.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            37099..38433
                     /gene="glrR"
                     /locus_tag="JN691_000913"
     CDS             37099..38433
                     /gene="glrR"
                     /locus_tag="JN691_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAY7242422.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            38494..38832
                     /gene="glnB"
                     /locus_tag="JN691_000914"
     CDS             38494..38832
                     /gene="glnB"
                     /locus_tag="JN691_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAY7242423.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(38877..40067)
                     /gene="hmpA"
                     /locus_tag="JN691_000915"
     CDS             complement(38877..40067)
                     /gene="hmpA"
                     /locus_tag="JN691_000915"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAY7242424.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            40394..41647
                     /locus_tag="JN691_000916"
     CDS             40394..41647
                     /locus_tag="JN691_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAY7242425.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(41845..43038)
                     /locus_tag="JN691_000917"
     CDS             complement(41845..43038)
                     /locus_tag="JN691_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY7242426.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGELWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            43336..46431
                     /locus_tag="JN691_000918"
     CDS             43336..46431
                     /locus_tag="JN691_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HAY7242427.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVANH
                     HIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKRFE
                     QYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNALLD
                     DAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPLAA
                     QDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRADFP
                     QAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSKAG
                     GYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQREK
                     LLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAAEM
                     LNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEECW
                     FARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAARYL
                     DNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLWHL
                     TGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHAAL
                     HYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYYND
                     QPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSLYG
                     DLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFNYV
                     H"
     gene            46528..>47036
                     /locus_tag="JN691_000919"
     CDS             46528..>47036
                     /locus_tag="JN691_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY7242428.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINC"
BASE COUNT        11348 a        11855 c        12498 g        11335 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgccagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctctgttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgtccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagcatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccgctcc agctatcgtt agccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtctgcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatctcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggctag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagcgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga cggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggta
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcact ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa attatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgacgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggctgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagagc ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca tcatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttagctt ttttcttctc ggcgcgtttt gccagtactt tcttcgatgg cttgccggtc
    11821 tgcttctcgc ttggcgcacg cgttttcggg cgtaactcat caataacgcg agctttaatc
    11881 ggctcttcaa tgtaacggcc aaccttgccc agcagcaaat ggtcatgggc ttccaccagc
    11941 gaaattgcgg tgcctttacg accggcgcgc gcggtacgtc cgatacgatg caaataagta
    12001 tcgccactgc gcggcatatc gaagttaaag acgtggctga cgtcaggaat gtcgataccg
    12061 cgcgcggcaa catcggtggc aaccagtacg tttacgcggc cttcggtcag gcgtttaatt
    12121 gcttcgttac gcttgccttg taccatctca ccttcgagat agcagttgtt gatgcccgct
    12181 tcgcgcagcc agtttgccag ctcatgcaca cgttcacgct tacgcacaaa cacgattgag
    12241 cgagtcgctt caggctgttt taacaggtgc accagcaatg cggttttatg ttccaggtca
    12301 tcggcgcggt aataccactg atgaattttt ttgcgctcac gggtggacgg attggcagaa
    12361 acttccaccg gatcttccag cagacgctcg gcaaagtcct gaatcgcatc gccttccagc
    12421 gttgccgaaa agagcagggt ctgtttacgc cagcgagttt cgccagcaat atgttcgata
    12481 tcctgagcga agcccatatc cagcatacgg tctgcttcgt cgaggatcag cgtttcaacc
    12541 gcgcggcaat cgaagttctc ttcttttatg tattgcagca gacgtccggt cgtggcgacc
    12601 acgatgtcct gattttcgct gaacacttcc gcgtggttca tataggctac gccgccggtg
    12661 atggtggcga tatccagatg cgtatgtttc gccagttcgc gggcatgatc ggccacctgc
    12721 atcgccagct cgcgagttgg ggtgaggatc aaaatacgcg gcggaccgga tttcttacgt
    12781 gggaaatcga gcaggtgctg caacgctggc agcagatacg ccgccgtttt accggtgcct
    12841 gtcggcgcag aaccgagtac atcacggcca tcgagcgcag gcggaatggc ggcagcctga
    12901 atggcggtcg ggcgagtgaa acctttatcc tggagggctt ccagcaggct ttcgtcgagt
    12961 tcaagttcgg aaaaagtcgt tacagtcatg ttctacctct gtgtggggcg ctgattatag
    13021 acgttacggc tgcaatcttc atctgtttgt atggatatcg cttctcgggt attgttctca
    13081 cccggtgcta tcgccgtttt tcctgcaagg ttttattttc atgtcacagt ctacatccgt
    13141 gcttcgtcgt aatggattta cttttaaaca gttttttgtt gctcacgatc gctgtgcgat
    13201 gaaagtggga acggatggca ttttgctggg cgcatgggca ccggtggctg gggtgaaacg
    13261 ttgccttgat atcggcgcgg gtagcgggtt gctggcactg atgctggcgc agcgaaccag
    13321 tgacagcgtg ataattgatg ccgttgagct ggaaagtgaa gctgccgcgc aggcgcagga
    13381 aaatatcaac cagtccccgt gggcagagcg gattaacgtc catacggcgg atattcagca
    13441 gtggatcaca cagcagacag tacgcttcga tttaatcatc agtaacccac cttactatca
    13501 gcagggagta gagtgcgcga cacctcaacg ggaacaggct cgctatacca ctacgcttga
    13561 tcacccatcg ctgctgacct gtgcggcgga gtgcattacc gaagagggat ttttctgcgt
    13621 ggtgctgcca gagcagattg gtaatggttt tactgaactg gcattaagca tgggctggca
    13681 tttacgtttg cggacggatg tggcggaaaa cgaagcgcga ctgccgcatc gggtgctgct
    13741 ggcattctcc ccgcaggcgg gagaatgctt tagcgatcgc ttagtcattc gtgggccaga
    13801 ccagaactat tccgaagcgt atacggcgct gacccaagct ttttatctgt ttatgtaatg
    13861 attgccgggg gaaaggatcg atggaccgga atgcgtgagc agttccgggt aatccagcgt
    13921 aaaatgcagc ccccggcttt ctttgcgcat catcgcgcag cgaacaatca actcggcaac
    13981 ctgtaccaga ttacgcagct ccagcaaatt atttgagacg cggaaatggg cgtaatattc
    14041 gtctatttct tgttggagca tggttatccg ccgcagggcg cgttccaggc gcttcgttgt
    14101 gcgcacaatg ccaacgtaat cccacataaa cagccgtagc tcgtgccagt tatgctgaat
    14161 tactacccgt tcgtcagggt tctcaacgcg gctttcatcc cacggcggta acgtactgac
    14221 gccgtgggca taaggcatac gtctggtgat atcttctgcc gccgaccagc catagaccag
    14281 acactccagc aatgaattcg aggccatgcg gttagcgccg tgtaagccgg tataactcac
    14341 ctcgccaatg gcatacaagc cctcgacgtc cgtacgccca tgatcatcaa ccattacacc
    14401 accgcaggta tagtgtgcag caggcacaat cggtaccggt tcttgtgtga gatcaatccc
    14461 cagcccgagc aatttttcat aaatcatcgg gaaatgctgg cgaataaaat cggcgggctt
    14521 atggctgatg tcgaggaaca tacaatctgc gccgaggcgt ttcatttcat ggtcaatggc
    14581 gcgggcgaca atatcgcgcg gggctagttc gccgcgctca tcaaaatcgg gcataaaacg
    14641 cgtgccatcc gggcgcttga gataagcgcc ttcgccgcgc agtgcttccg ttaacaggaa
    14701 attgcgtgcc tgtgggtgat acagtgcggt agggtggaac tgattaaatt cgagattggc
    14761 aacccgacag cccgcgcgcc acgccatagc aatgccatcg ccagaagaaa tatccggatt
    14821 ggtggtgtac tgatacacct tcgacgcacc gccggttgcc agtaccactg cttttgcgtg
    14881 gcaggtttcc accgtttctt tatttcggtt ccatacccac gcgcctacaa cccgtcgcgt
    14941 gcccggcagg ccaattttgt cagagacaat cagatcaact gcgttgctgc gctccagcac
    15001 acgaatattc ggatggttct gcgccttgct caccagcgtg gattgtactt ctctaccggt
    15061 ggcgtcggcg gcatgaagaa tacgacggtg actatgtcca ccttcacggg tcagatggta
    15121 gctttcttcg ccattcggtt gaacgtgggt atcaaacaaa accccctggt caattaacca
    15181 ttgcacacag gaacgagcat tgctggcgac aaattcaact gcatggcgat cgcaaatacc
    15241 agccccggca atcaatgtgt cttccacatg cgagtcaatg ctgtcagttt catcaaacac
    15301 ggcggcaata ccgccctggg cataaaatgt tgaaccttcc gttaccgggc ctttacttag
    15361 aacgatgacc tgatgctggt cagccaggcg tagcgccagt gaaagtccgg ctgcgccgct
    15421 accaataatc aacacgtcac atgaatgttc agggagagta ttcattttct ttgtttaatt
    15481 tactaaacat tgtttggtca gcatagcatc atgttgtgcg aataaacacc tgctatttta
    15541 atatttgtta cagtggctaa acacgttgac gcagggcggc gagaaaaaga gaagttactg
    15601 gctggtggag gattaggtgg tgaaataaaa aggccgttgg gttactcttc aggcagttaa
    15661 atgggcattt ctacacagat aatgcgatgt tcagattctg tagacttata atgatagata
    15721 atgatccgtc tacagcatga caaacaaaaa cagatgcgtt acggaacttt acaaaaacga
    15781 gacactctaa ccctttgctt gctcaaattg cagctaatgg agtggtgttt cgatagcgcg
    15841 tggaaatttg gtttggggag actttacctc ggatgagcga gcagttaacg gaccaggtcc
    15901 tggttgaacg ggtccagaag ggagatcaga aagcctttaa cttactggta gtgcgctatc
    15961 agcataaagt ggcgagtctg gtttcccgct atgtgccgtc gggtgatgtt cccgatgtgg
    16021 tacaagaagc ttttattaaa gcctatcgtg cgctggattc gttccgggga gatagcgctt
    16081 tttatacatg gctgtatcgg attgctgtaa atacagcgaa aaattacctg gttgctcagg
    16141 ggcgtcgtcc accttccagt gatgtggatg ccattgaagc tgaaaacttc gaaagtggcg
    16201 gcgcgttgaa agaaatttcg aaccctgaga acttaatgtt gtcagaagaa ctgagacaga
    16261 tagttttccg aactattgag tccctcccgg aagatttgcg catggcaata accttgcggg
    16321 agctggatgg cctgagctat gaagagatag ccgctatcat ggattgtccg gtaggtacgg
    16381 tgcgttcacg tatcttccga gcgagggaag ctattgataa caaagttcaa ccgcttatca
    16441 ggcgttgacg atagcgggat actggataag ggtattaggc atgcagaaag aacaactttc
    16501 cgctttaatg gatggcgaaa cgctggatag tgagctgctt aacgaactgg ctcataaccc
    16561 agaaatgcag aaaacctggg aaagctatca cttaatccgt gattcaatgc ggggtgatac
    16621 tcccgaggtg ctccatttcg atatctcttc acgcgtgatg gccgccattg aagaagagcc
    16681 agtacgtcaa ccggcgacat tgatcccgga agcccagcct gcgccgcatc aatggcagaa
    16741 aatgccattc tggcagaaag tacgtccgtg ggcggcacag cttacccaaa tgggcgtagc
    16801 cgcatgcgta tcgcttgcag ttatcgttgg cgtccagcac tataatggac aatctgaaac
    16861 gtcccagcag cccgaaacgc cggtatttaa tacactgccg atgatgggta aagccagccc
    16921 ggtaagcctg ggagtacctt ctgaagcgac cgcaaacaat ggtcaacagc agcaggtaca
    16981 ggagcagcgt cgtcgcatta atgcaatgtt gcaggattac gaactgcaac gccgactcca
    17041 ctctgaacag cttcagtttg agcaggcgca aacccagcaa gccgctgtac aggtgccagg
    17101 aattcaaact ttaggaacgc aatcgcagta atgaagcaac tttggtttgc catgtcatta
    17161 gtgacaggta gcctgttatt ctctgctaac gcctcggcca ctcccgcgtc cggggcgtta
    17221 ttacagcaga tgaacctggc cagtcagtca ctgaattacg agctgtcatt catcagcatc
    17281 aataaacagg gtgttgagtc tctgcgttat cgacatgcac gcctcgataa ccgtcctctt
    17341 gcacaattgt tgcaaatgga tggcccgcgc cgggaagtgg tacagcgcgg caatgaaatc
    17401 agctattttg aaccgggact tgaaccgttc acgcttaatg gcgattacat tgttgattct
    17461 ctgccatcgc ttatctatac cgatttcaaa cgcctttctc cttactacga ctttatctcc
    17521 gtcgggcgca cgcgtattgc tgatcgtctt tgcgaagtca ttcgcgtggt tgcccgagat
    17581 ggtacacgct acagctacat cgtgtggatg gacaccgaat cgaaattacc gatgcgggtt
    17641 gatctccttg atcgcgatgg tgaaacgctg gaacaatttc gcgtgattgc ttttaacgtc
    17701 aatcaggata tcagcagcag tatgcagacg ctggcgaagg caaatttgcc gccgttgctt
    17761 tctgttcctg taggtgaaaa agctaaattc agctggacgc caacctggtt gccacagggt
    17821 tttagcgaag tttccagtag tcgacgtccg ctaccgacga tggacaacat gcctatcgaa
    17881 tcacgtctct attccgacgg attattcagc ttctcggtaa acgttaaccg cgctacgcca
    17941 tcgagcaccg atcagatgtt gcgcaccgga cgcagaaccg tcagtacaag cgtacgtgat
    18001 aacgccgaaa tcaccattgt cggtgaactg ccgccgcaaa cggcgaaacg cattgccgag
    18061 aatattaagt tcggggcagc gcaatgatca aagagtgggc taccgtcgtc tcctggcaaa
    18121 acgggcaggc gctggtcagt tgtgatgtta aagcttcatg cagcagctgc gcttcacgcg
    18181 ccggttgcgg tagccgcgtg ttgaataaac ttggcccgca aaccacgcat accattgtcg
    18241 taccctgtga tgagccgtta gtgccggggc aaaaagtgga attggggatc gccgaaggca
    18301 gcctgcttag ctccgcatta ctggtttata tgtcgccgct ggtgggatta ttcctcatcg
    18361 cttcgctatt tcagctactc tttgcttctg acgtcgcagc attatgtggt gcggtcctcg
    18421 gcggtattgg cgggttcctg attgcgcgcg gctactcgcg taagtttgct gcccgggcag
    18481 aatggcaacc gatcatctta agcgtggcat tgccgcctgg ccttgtgcga tttgaaacat
    18541 cttcggaaga cgcgagccag tgatttcact ggctttgttg cattttgcat aacaaatgcg
    18601 aggacgtttc cagataccac aatccatacg gtatgaacat ttcctcgcct caatgttgta
    18661 gtgtagaatg cggcgtttct attaatacag acgttaagct cagaacagcg acttctaaaa
    18721 gcctggttaa ccgggcatta aggcacaata atcatacttt atgaagaata tacgtaactt
    18781 ttcgatcata gctcacattg accacggtaa atcgacgctg tctgaccgta ttatccagat
    18841 ctgcggtggc ctgtctgacc gtgaaatgga ggcgcaggtt ctcgattcca tggatcttga
    18901 gcgtgagcgt ggcattacca tcaaagcgca aagcgtgacg ctggactaca aagcgtctga
    18961 cggcgaaacc tatcagctta actttatcga caccccgggc cacgtagact tctcctatga
    19021 agtttcccgt tcgctggctg cctgtgaagg tgcattgctg gtggtcgacg ccgggcaggg
    19081 cgtagaagcg caaaccttgg caaactgcta caccgccatg gaaatggatc tcgaagtggt
    19141 gccggtactg aacaagattg acctgccggc agccgatcct gaacgcgtgg cggaagaaat
    19201 tgaagatatc gtcggcatcg acgccaccga tgcagtgcgc tgttcagcga aaaccggcgt
    19261 tggcgtgcag gacgttctcg aacgtctggt gcgcgacatt ccgccgccgg aaggcgatcc
    19321 ggaaggcccg ttgcaggcac taattatcga ctcctggttc gacaactacc tgggcgttgt
    19381 ttcacttatc cgtattaaaa acggcaccct gcgtaagggc gacaaagtga aagtcatgag
    19441 taccgggcag acctataacg ccgaccgtct gggcatcttc acgccgaaac aggttgaccg
    19501 cactgaactg aaatgtggcg aagtaggctg gctcgtatgt gcgattaaag atatccacgg
    19561 cgctccagtc ggcgatacct taacgctggc gcgtaatccg gcagaaaagg cgctgcctgg
    19621 ctttaagaaa gtcaaaccgc aggtatacgc cggtctgttc ccggtaagtt ccgacgacta
    19681 tgaagccttc cgtgacgcgc tgggtaaact cagcctgaac gatgcctcac tgttctatga
    19741 gccggaaagc tccagcgcgc tgggctttgg tttccgctgc ggcttccttg gcctgctgca
    19801 catggagatc atccaggagc gtctggaacg tgaatacgat ctggatctga tcaccactgc
    19861 gccgaccgta gtgtatgaag ttgaaaccac gtcaagggaa gttatctacg tcgacagccc
    19921 atccaagctg cctgcggtaa ataacattta cgaactgcgc gagccgattg cagagtgtca
    19981 catgctgctg ccgcaggcat atctcggcaa cgttattacg ctgtgcgtag aaaaacgcgg
    20041 cgtgcagacc aatatggttt accacggtaa tcaggtggcg ctgacgtacg agatcccgat
    20101 ggcggaagtg gtgctcgact tcttcgatcg cctgaaatct acctcgcgtg gttatgcgtc
    20161 tctggattac aacttcaaac gcttccaggc gtccgacatg gtacgtgtag acgtattaat
    20221 caacggtgaa cgtgttgatg cgctggcgtt gatcacccac cgtgataatt cgcaaaaccg
    20281 cggtcgcgag ttggtggaga agatgaaaga tctgatccca cgccagcagt ttgatatcgc
    20341 cattcaggca gcgattggta cgcacattat tgcgcgatcc accgtgaaac agctgcgtaa
    20401 aaacgtactg gctaaatgtt atggcggcga tatcagccgt aagaaaaagc tgctgcagaa
    20461 gcagaaagaa ggtaagaaac gcatgaagca gatcggtaac gttgagctgc cgcaggaagc
    20521 gttcctcgcc attctgcacg tcggcaaaga caacaaataa cccttaggag ttggcatggc
    20581 gaatatgttt gccctgattc tggtgattgc cacactggtg acgggcattt tatggtgcgt
    20641 ggataaattc tttttcgcac ctaaacggcg ggaacgtcag gcagcggcgc aggcggctgc
    20701 cggtgactca ctggataaag caacgttgaa aaaggttgca ccgaagcctg gctggctgga
    20761 aaccggagct tctgtttttc cggtgctggc tatcgtattg attgtacgtt cgtttattta
    20821 tgaaccgttc cagatcccgt caggttcgat gatgccgact ctgttaatcg gtgattttat
    20881 tctggtagag aagtttgctt atggcattaa agatcctatc taccagaaaa cgctgatcga
    20941 aaccggtcat ccgaaacgcg gcgatatcgt ggtctttaaa tatccggaag atccaaagct
    21001 tgattacatc aagcgcgcgg tgggtttacc gggcgataaa gtcacttacg atccggtctc
    21061 aaaagagctg acgattcaac cgggatgcag ttccggccag gcgtgtgaaa acgcgctgcc
    21121 ggtcacctac tcaaacgtgg aaccgagcga tttcgttcag accttctcac gccgtaatgg
    21181 tggggaagcg accagcggat tctttgaagt gccgaaaaac gaaaccaaag aaaatggaat
    21241 tcgtctttcc gagcgtaaag agacactggg tgatgtgacg caccgaattc tgacagtgcc
    21301 gattgcgcag gaccaggtgg ggatgtatta ccagcagcca gggcaacaac tggcaacctg
    21361 gattgttccg ccgggacaat acttcatgat gggcgacaac cgcgacaaca gcgcggacag
    21421 ccgttactgg ggctttgtgc ctgaagcgaa tctggtcggt cgggccacgg ctatctggat
    21481 gagcttcgat aagcaagaag gcgaatggcc gactggtgtg cgcttaagtc gcattggcgg
    21541 catccattaa tagccatctt cgttcacgtt gtcgccgtta tggcgacaac gtgaattatt
    21601 tatgagataa atctcccgtg gctaacgaca tcccccgtcg ttgtgtatag aatattcccc
    21661 cgaagtttaa ggttggcacc tccaggttgc cacggcacac gaaacagcgt tggtccccat
    21721 ataccgctaa actgaaactg cagcgaagca gttagcagag ccatgtatat caggtctgtt
    21781 tcgtgtgctg aattgttgac gcatttattt attggtatcg catgaacccc atcgtaatta
    21841 atcggcttca acggaagctg ggctacactt ttaatcatca ggaactgttg cagcaggcat
    21901 taactcatcg tagtgccagc agcaaacata acgagcgttt agaattttta ggcgactcta
    21961 ttctgagcta cgttatcgcc aatgcgcttt atcaccgttt ccctcgtgtg gatgaaggcg
    22021 atatgagccg gatgcgcgcc acgctggtcc gtggcaatac gctggcggaa ctggcgcgcg
    22081 aatttgagtt aggcgagtgc ttacgtttag ggccaggtga acttaaaagc ggtggatttc
    22141 gtcgtgagtc aattctcgcc gacaccgtcg aagcattaat tggtggcgta ttcctcgaca
    22201 gtgatattca aaccgtcgag aaattaatcc ttaactggta tcaaactcgt ctggacgaaa
    22261 ttagcccagg cgataaacaa aaagatccga aaacgcgctt gcaagaatat ttgcagggtc
    22321 gccatctgcc gctgccgact tatctggtag tccaggtacg tggcgaagcg cacgatcagg
    22381 aatttactat ccactgccag gtcagcggcc tgagtgaacc ggtggttggc acaggttcaa
    22441 gccgtcgtaa ggctgagcag gctgccgccg aacaggcgtt gaaaaaactg gagctggaat
    22501 gagcatcgat aaaagttact gcggatttat tgccatcgtc ggacgtccga acgttggcaa
    22561 atccacattg ttgaacaaac tgctggggca gaaaatctcc atcacctccc gcaaggcgca
    22621 gacaactcgt caccgcattg tggggatcca tactgaaggc gcgtatcagg cgatctacgt
    22681 cgatacaccg ggcctgcata tggaagaaaa acgcgccatt aaccgcctga tgaacaaagc
    22741 ggcgagcagc tctattggcg atgttgagct ggtgattttt gtcgttgaag gcacccgctg
    22801 gacgccggac gacgaaatgg tgctcaacaa actgcgcgaa ggcaaagcgc cgataatcct
    22861 cgcggtgaac aaagtggaca acgtgcagga gaaagccgat ctgctgccgc acctgcagtt
    22921 cctggcaagc cagatgaact tcctcgatat cgtgccaatc tctgccgaaa ccgggctgaa
    22981 tgttgacact attgcggcaa tcgtgcgtaa gcatctgcct gaagcgactc atcacttccc
    23041 ggaagattac atcaccgatc gctcacagcg ttttatggcg tctgaaatca tccgcgaaaa
    23101 actgatgcgt ttccttggcg ctgaactgcc gtactccgtg accgtggaga tcgaacgttt
    23161 cgtctctaac gaacgcggcg gttatgacat caacggtttg attctcgttg agcgtgaagg
    23221 gcagaagaag atggttattg gcaacaaagg ggccaagatc aaaaccatcg ggattgaagc
    23281 gcgtaaagac atgcaggaaa tgttcgaagc gcctgtccac cttgaactgt gggtaaaagt
    23341 gaaatcgggt tgggccgacg acgaacgcgc actgcgcagt ctcggttatg ttgacgatct
    23401 ttaagagtca ctccgatgga aggctggcag cgcgcatttg tcctgcatag tcgcccgtgg
    23461 agcgaaacca gcctgatgct ggacgtcttc acagaggaat cgggtcgcgt gcgtctggtt
    23521 gccaaaggcg cacgctctaa acgctctact ctgaaaggtg cattacagcc tttcacccct
    23581 ctcttgctac gttttggcgg gcgtggcgaa gtcaaaacgc tgcgcagtgc tgaagctgtc
    23641 tcactggcgc tgccattaag cggtatcacg ctttacagcg gtctgtacat caacgaactt
    23701 ctctcccgcg tactggaata cgagacgcgc ttctctgaac tctttttcga ttacttgcac
    23761 tgcattcagt ctcttgcagg tgccactggt acgccagaac ccgcgctgcg ccgctttgaa
    23821 ctggcactac tcgggcatct gggctatggc gtcaatttta cccattgcgc gggtagcggc
    23881 gagccggtag atgacaccat gacgtatcgt tatcgcgaag aaaaagggtt tatcgcaagc
    23941 gtcgttatcg acaataaaac gttcaccgga aggcagttaa aagcgttaaa cgcgcgggaa
    24001 tttcctgacg cagacacact acgcgccgcg aaacgcttta cccgaatggc gcttaagccg
    24061 tatcttggcg gtaaaccatt aaagagcagg gaactgttcc ggcagtttat gcctaagcga
    24121 acggtgaaaa cacattatga atgatgagga ttgtcatggc tgaattactg ttaggcgtca
    24181 acattgacca tattgcaacg ctgcgtaacg cacgcggtac tgcttacccg gatccggtgc
    24241 aggcggcgtt tattgccgag caggcgggag cggacggcat taccgtgcat ttacgtgaag
    24301 atcgccgcca cattactgac cgcgatgtgc gcatcctgcg gcagacgctg gatacccgaa
    24361 tgaatctgga gatggcagtg accgaagaga tgctggcgat cgccgttgag acgaagccac
    24421 atttttgctg cttggtaccg gaaaagcgtc aggaagtaac aaccgaaggc ggcctggacg
    24481 ttgcagggca gcgtgaaaaa atacgcgatg cctgcaaacg tctggcagat gccgggattc
    24541 aggtttctct gtttatcgac gccgatgaag agcagatcaa agctgcggca gaggttgacg
    24601 caccgtttat cgagatccac accggttgct atgctgatgc caaaactgac gccgaacagg
    24661 cgcaagagct ggtgcgtatc gccaaagccg cgacctttgc cgctagtctc ggtctgaaag
    24721 ttaacgccgg acacggtctg acctatcaca acgtgaaagc cattgccgcc atccctgaga
    24781 tgcatgaact gaatatcggt catgccatta ttggccgtgc agtgatgacc ggactgaaag
    24841 atgcggtggc agaaatgaag cgtctgatgc tggaagcgcg tggctaatgg caatattagg
    24901 tttaggcacg gatatcgtag agatcgctcg catagaagca gtgatcgccc gatccggtga
    24961 gcgcctggca cgccgcgtat taagcgataa cgaatgggca atctggaaaa cgcaccacca
    25021 gccggtgcgt tttctggcga agcgttttgc tgtgaaagaa gccgcagcaa aagcgtttgg
    25081 caccgggatc cgcaatggtc tggcgtttaa tcaatttgaa gtattcaatg atgagctcgg
    25141 caaaccacgg ctacggctat ggggtgaggc attaaaactg gcggaaaagc tcggcgttgt
    25201 aaatatgcat gtaacgctgg cagatgaacg ccactatgct tgtgccacgg taattattga
    25261 aagttaaaat aataccgttc tctttaaaaa gtaaaacccc cgaactggta aggaacgggg
    25321 gttttacttt ttaactttaa ttttgtgcag catgcaccct caacaacagc gattatggat
    25381 cagtgtactt atcttttaca agcaacttgc aatctttagc ataaaaactc gagcctttac
    25441 gaagaaagca atattgatgg aaagattaac gtgaccgcca attcgtaagt acattaaaat
    25501 tggcttcgtt attgaagatt ttgctgtgct ttacaccatg ccacagaatt cccccattga
    25561 aacgagtggt gtcgtcaaag ctctggtgtg gagtgcagca tgcaccctca ataactcgca
    25621 cgttcagttt tggggagatg taagggctaa tctgaatggc tgcattcctt gtttaaggaa
    25681 aaacgaatga ctgattgccg atacctgatt aaacgggtca tcaaaatcat cattgctgtt
    25741 ttacagctga tccttctgtt ctcataacac aaggaaacgt acttaaggtg cgtccggtga
    25801 accaatcgga cgcgccttta ataactataa ataagtgtct tggcagatac tatataaatt
    25861 aacttagtga atgattatgc taatgtcatc aattaaataa atataatggc gttaaggctt
    25921 cccagtaata taattaatac tctacttcca gagtagaata ttaaatttta tccgcgtggt
    25981 gcatcagcac aaatttatcc cacaactgtt cttctgtctc gacatgcgcc ggatctttca
    26041 caatagtatt ggggatcggg cacaccttct ggcaggttgg tgtctcgtag tgccctacgc
    26101 attcggtaca cttatcgctg ttaatctcgt agatatgatc tcccattgaa atcgcctcat
    26161 tcgggcattc gggttcacac atatcacaat tgatgcattt tttagtgatt aacagcgcca
    26221 taaggaaact ctcaaaacaa cacaaaccgg gcgggcatta tacgcgcatt ctttactcag
    26281 accagttttt tcaccagcgc ttcactatga cgaatacgtt ctggtgccag ttccagatcc
    26341 tgctgaatca gcgcaatgaa cagcaaatcc gttaacattc cctgagcgtg acaagcagag
    26401 attgaagcac tgtttgtcgc ttgttcttcg gcaatggtat acaggcaatg agaagcacgc
    26461 tgttgcaggg cattcggagt aaagccggta atcgccagca cttttccgcc cactcgcagc
    26521 atctcatctg ccgccaggtt taactcgcgt cgtacaccgg tgtaggaaat ggctaataac
    26581 agatcgtcag gggacgacgc ctgtactgtt gcgagtagcg catgcatatc gcgcagtgcg
    26641 gcagcattga agccaatctt catcagcttc caggcaaagt tttgcgccac cagacccgaa
    26701 gcgccaatac cggtcagaat tatccgccgc gcagagcgca acattgttac gcattcatgc
    26761 agtttctctt cactattaac gtttagcgtt gcgtacatag cggcagtatt ttctttaatc
    26821 agtttttcgc cgaccagccg taacggatca tcaccgcgga tttggttatg aatgggcacg
    26881 gagggcgatt ccggctggct tgccagcgct tcactcaacg ccaacttaag cgccggaaaa
    26941 cctttatagc cgagtttttg cgcgaacttc acgacgctgg actgactgac tccggcttcg
    27001 ttggccagtt gctgagagct taaatggcgc gccgtatcag gttgtagcag cagataatcc
    27061 gccagttttt tatcactctg ggcaagcccc tggtaacgct gacggatacg aagtaagccg
    27121 ttcatatctt tcctcgcgat gaaatagtgg cgacaacagg caatatgaga cacacgcgag
    27181 tgaattttat attccattgc cgctaattgc catgaattaa aaattccagt tagccatgat
    27241 gcggtcgatt gttgccggaa aagtgatgat ctatctgtta ggctatgtag tagcgaaaat
    27301 taatcaggat gtttcagtcc agaggagtat ggtttggcaa ctcacgagcg tcgtgtggtg
    27361 ttttttgact tagatggaac attgcatcag caggatatgt tcggcagttt tctgcgctat
    27421 ttactgcgtc gccaaccgct gaatgcgtta cttgtcctac cgttgttacc gattatagcc
    27481 attgcgttat tgataaaagg tcgtgcggca cgctggccga tgagtctgct tctgtggggg
    27541 tgcacttttg gtcacagcga agcacgttta cagacgttgc aggccgattt cgtgcgctgg
    27601 tttcgcgaca atgttaccgc ctttccgctg gttcaggagc gattaaccac ctacctgtta
    27661 agttccgatg ctgatatctg gttgattacc ggctctccgc agccgctggt tgaagcggtt
    27721 tatttcgata cgccctggct gccgcgggtt aatcttatcg ccagccaaat tcagcgtggc
    27781 tatggtggtt gggtattgac gatgcgttgt ctgggacatg aaaaggtcgc acaactggag
    27841 cgcaaaatcg gcacgccgct gcggctgtac agtggctata gcgacagtaa tcaggacaat
    27901 ccgctgcttt atttctgtca gcatcgttgg cgagtaaccc cgcgcggtga actccagcaa
    27961 ctggaataga gtaaagcata gcgtccgtgt ataatgcgcc gcgcttttat aaccggagtt
    28021 ttctttttgt ctgaagtcga atttagccac gaatactgga tgcgtcacgc gctgacgctg
    28081 gcgaaacgtg cctgggatga gcgggaagtg ccggtcggcg cggtattagt gcataacaat
    28141 cgggtaatcg gcgaaggctg gaaccgcccg attggtcgcc atgatcccac cgcacatgca
    28201 gaaatcatgg ccctgcggca gggtggtctg gtgatgcaaa attatcgtct gatcgacgcc
    28261 acgttgtatg tcacgcttga accatgtgta atgtgtgccg gagcgatgat ccacagtcgc
    28321 attggtcgcg tggtctttgg tgcgcgtgac gcgaaaactg gcgctgcggg atctttaatg
    28381 gatgtgctgc atcatccggg tatgaatcac cgagtggaaa ttacggaagg aatactggcg
    28441 gatgagtgcg cggcgttgct cagtgacttc tttcgcatgc gccgccagga aattaaagcg
    28501 cagaaaaaag cgcaatcctc gacggattaa ttttgtttct cttcactccc tttcctggaa
    28561 aacagcagag agggagaatg ggtcaaatag tttgatgacg actgggaaag aaacgagaga
    28621 aaaggaaatt tctctttgcc caactccgta ggcgataccg ccggataatc ctgcgccagt
    28681 tgcatcgccg cttctgtagc ctgcttctct ttctcttgca gataacccac caggctaatc
    28741 agatacttac gaatattttc gacataagcg taggcttcat gcccacgagc gtagccgtaa
    28801 gtcagcttgc tgtaataggg tttctggcta agtaaaggca gacgctgttt tacgtcagcc
    28861 caactgtcag gattcccttt ggtttttgcc gtcagggcgc gggcatccag catatgcgca
    28921 tagcccatat tgtacgcagc gagggcaaac cagatccgtt cgttctccgg cacactttcc
    28981 ggcactttac tcatcatatc ctgcaaataa cgcacgccac cgctgatgct ctgttcggca
    29041 tcggtacgat ccgtaatgcc gaggctttgc gcggtatttt tggttaacat catcatgccg
    29101 cgcacacccg tcggtgaagt ggcctgtgca tcccagtgcg attcctgata agcaatagcg
    29161 gccagcaaac gccagtcaat ttcttcggcg tatttctcaa acaggggctt taactgcggc
    29221 agtaccgcat cgacggcgcg taaaaatgtg cgcgtatcga cgtaatcaaa atcatcgcca
    29281 tgccccaggt atttctcttc aatgcgtgcc agcgtaccgt cttcattcat ttcgttgaag
    29341 aagtcgagca gggcggcgga aagggtatta tcgccatcta acgggctaaa ccaagtcacc
    29401 ggttgttcat cggtgatatc gagcgctacg gcgagctccg ggtgaacgcg ctgaaacagg
    29461 ctgatggcga cagaatcagc aatggtgtaa tcgagttttc cttcgatgac atcttccatt
    29521 aattccgcag agcctttttt gtcgtctacc ttccagctta attccgggaa ttttgtttct
    29581 ttcagggtct ggagatcgtt aaccaccaca tgacccggtg caacggtaag ttgctccgcc
    29641 gtcaggttgc ccagcgtacg tgggcgatac tgacccactt tataaaccag ttgttgtgac
    29701 acggaataat aggtagggcc aggctgataa ttttttaccc gctcactgtt atagaccagt
    29761 cctgccgcca gcaggtcggc gttaccatta tcaaggtcgt caaacagctg gctgatattc
    29821 tgccgcacgg tcactttcag ttttacgccg aggtaatcgg caaactgttt cgccagttcg
    29881 taatccaggc caaaaggttt cccgttgatt tcgttataag tcaggggagt atgaatggtg
    29941 ctcacacgca actctccccg cgcttgaatg gcggcgatac ggttgtcggc tttaccaaac
    30001 cagggaatgg atggccagag agcgaccgcg agcagcagtg ccagaatgcc gatgaacaga
    30061 taattaatct ttaatttttt caattagtta attctctgtg tcgtgcgcgt cccagcttga
    30121 aaaaacgtaa taatagtgaa aggtttactc ataaatgagc ggcattttgc gtaaacctgc
    30181 gccagatggc aacttattac agccattggc ggcacgcgtt gctaattcac gatggtgatt
    30241 ttatttccac gcaaacggtt tcgtcagcgc atcagattct ttataatgac gcccgtttcc
    30301 cccccttggg tacaccgaaa gcttagaaga cgagagactt atgatggaaa ttctgcgtgg
    30361 ttcgcctgca ctgtcggcat tccgaatcaa caaactgctg gcacgttttc aggctgccag
    30421 gctcccggtt cacaatattt acgccgagta tgtccatttt gctgacctca atgcgccgtt
    30481 aaacgatgat gagcacgcac aacttgaacg cctgctgaaa tatggtccgg cactcgccag
    30541 ccacgccccg caaggcaaac tcctgctggt gaccccgcgt cctggcacca tctccccctg
    30601 gtcttcgaaa gcgaccgata ttgcccataa ctgcgggcta caacaggtaa accgccttga
    30661 gcgcggcgtt gcttactata tagaagccgg tacgctgacc aatgaacaat ggcagcaggt
    30721 taccgctgaa ctgcacgacc gcatgatgga aacggtcttt tttgatttag atgatgcaga
    30781 gcagttgttt gcccaccatc aaccgactcc ggtcacaagc gttgatttgc tggggcaggg
    30841 ccgtcaggcg ctgatcgacg ctaacctgcg tcttggcctg gctctggcgg aagatgaaat
    30901 tgactatctg caggatgctt tcacaaagct tggtcgtaac ccgaacgaca tcgagctgta
    30961 tatgttcgct caggcgaact ctgagcactg ccgccacaaa atttttaacg ccgactggat
    31021 tatcgacggt gaacaacagc cgaaatcgct gttcaggatg atcaaaaata ctttcgaaac
    31081 cacgccagat cacgttctct ctgcttataa agataacgcc gccgtaatgg aaggttctga
    31141 agtgggccgc tactttgctg accacgaaac gggccgctac aatttccatc aggaaccggc
    31201 gcacatcctg atgaaagtcg aaactcacaa ccacccgacg gcgatttctc cgtggccggg
    31261 cgcggcgacc ggttccggcg gtgaaatccg cgatgaaggt gccaccgggc gcggcgcgaa
    31321 gccgaaagct ggtttggttg gtttctccgt atccaacctg cgaattcctg gcttcgaaca
    31381 gccgtgggaa gaagatttcg gtaagcctga gcgcattgtt accgcgctgg acatcatgac
    31441 cgaaggcccg ctgggcggtg cggcgtttaa caacgaattt ggtcgtccgg cactgaacgg
    31501 ctacttccgt acttatgaag aaaaagtgaa cagccacaat ggcgaagagc tgcgcggtta
    31561 tcacaaaccg atcatgctgg cgggcgggat cggcaacatt cgcgccgatc acgtacaaaa
    31621 aggcgagatc aacgtcggtg cgaagctggt cgttctcggc ggcccggcaa tgaacatcgg
    31681 tcttggcggc ggcgcggcgt cttccatggc gtctggtcag tctgatgccg acctcgactt
    31741 tgcttccgta cagcgcgaca acccggagat ggagcgtcgt tgccaggaag tgatcgaccg
    31801 ttgctggcag cttggtgatg ctaacccaat tctgtttatc cacgacgtcg gtgcgggcgg
    31861 tctttctaac gcgatgccgg agctggtgag cgacggcgga cgcggcggta aatttgaact
    31921 gcgcgatatt ctaagcgacg aaccgggcat gagcccgctg gaaatctggt gtaacgaatc
    31981 ccaggaacgc tacgtgctgg cggttgctgc cgatcaatta ccgctgtttg acgaactgtg
    32041 taagcgtgag cgcgcaccct acgcggtgat tggtgaagcg accgaagaac tgcatctttc
    32101 tctgcacgat cgtcattttg ataatcagcc gatcgatctg ccgctggacg tcctgcttgg
    32161 taaaacgccg aagatgaccc gcgatgtaca aacgctgaaa gcgaagggcg atgcgctggt
    32221 ccgtgaaggg atcaccattg cagacgcggt gaaacgcgta ctgcatctgc cgaccgtggc
    32281 ggagaaaact ttcttggtga ccatcggtga ccgcagcgta accggcatgg tggcgcgcga
    32341 tcagatggtg gggccgtggc aggtgccggt cgctaactgc gcggtcacta ccgccagcct
    32401 cgacagctac tacggtgaag cgatggcgat tggcgagcgt gcgccggttg cgctgctgga
    32461 tttcgccgcc tctgcccgtc tggcggtcgg tgaagcgtta accaatattg ccgcaacaca
    32521 gattggcgat atcaaacgca ttaaactttc cgccaactgg atggcggcgg caggccaccc
    32581 tggtgaagat gcgggcctgt atgaagccgt taaagccgtg ggtgaagaac tttgtccggc
    32641 gctgggcctg acgatcccgg tgggtaaaga ctctatgtcg atgaaaaccc gctggcagga
    32701 aggtaacgaa gagcgcgaaa tgacgtcgcc gctgtcgctg gtgatttctg catttgcccg
    32761 cgtggaagat gtacgtcaca ccatcacgcc gcagctttgt accgaagata acgcactgct
    32821 gctgattgat ttgggcaaag gcaataacgc gctgggcgca acggcgctgg cgcaggttta
    32881 tcgtcagctt ggcgacaaac cggcagatgt acgcgatgtt gcccaactga aaggcttcta
    32941 tgacgcgatt caggcgctgg ttgcacagcg taagctgctg gcgtatcacg accgctctga
    33001 tggcggcctg ctggtaacgc tggcggaaat ggcctttgct ggtcattgtg gcattgacgc
    33061 ggatatcgcc tctctgggtg acgatcgcct ggcggcgttg tttaacgaag aactgggtgc
    33121 ggtgattcag gttcgtgccg ctgaccgtga agcggtcgag tccgtactgg cacagcatgg
    33181 gcttgcggat tgcgtccatt atgttgggca ggcggtttcc ggtgaccgtt ttgtgattac
    33241 cgccaacggg cagactgtat tcagcgaaag ccgcactacg ttgcgtgtct ggtgggcaga
    33301 aactacctgg cagatgcagc gcctgcgtga caacccggag tgtgccgatc aggagcatca
    33361 ggcgaaatct aacgacgccg atccgggcct gaatgtaaaa ctgtcgttcg atatcaacga
    33421 agatgtggca gcaccgtata ttgccactgg cgcacgtccg aaagttgccg tactgcgtga
    33481 gcagggcgtg aactcgcatg ttgaaatggc ggcagctttc caccgtgggg gctttgatgc
    33541 tatcgacgtg catatgagcg acctgctgac tggacgtacg ggactggagg attttcacgc
    33601 cctggtcgcg tgcggaggtt tctcctacgg tgatgtgctg ggtgccggtg aaggttgggc
    33661 gaagtctatc ctgttcaatg accgtgtacg cgatgagttt gcaaccttct tccatcgtcc
    33721 gcaaacgctg gccctggggg tatgtaacgg ttgccagatg atgtctaacc tgcgtgaact
    33781 gatcccgggg agcgagttgt ggccacgttt tgtgcgtaac acctccgatc gctttgaagc
    33841 gcgtttcagc ctggttgaag taacccaaag cccgtctctg ctgttgcagg ggatggtggg
    33901 ctcgcaaatg ccgattgctg tctctcatgg tgaagggcgc gtggaagtgc gtgatgcggc
    33961 gcatctggcg gcactggaaa gcaaagggct ggtggcactg cgctatgtcg ataacttcgg
    34021 caaagtcact gaaacctacc cggctaaccc gaacggttca ccgaacggta ttacggcggt
    34081 tacgactgaa agtggtcgag tcaccattat gatgccgcac ccggaacgtg ttttccgtac
    34141 tgtcagcaac tcctggcatc cggaaaactg gggcgaggat ggcccatgga tgcgcatttt
    34201 ccgcaatgcg cgtaagcagt tggggtaagt catcagttca ttggttttgc agcccggatg
    34261 cggcatttac gccgcatccg gcattaagca ccaaccacta tcataagctc ccaactccgg
    34321 gagctttttt gtgtctgtaa atcacgacaa tgggtggttt accgtgtcgc tttctggcga
    34381 cacttaaatg attgatttta ttgtaatcta ataagtttat cttaagttgt ctctttttag
    34441 cgacacagtg gctgatttac attcaaatag cgaagaagcc ataaatccct caaagcaacg
    34501 tataatcagt acgttaccaa actattttct ttattggcac agttactgca taatagtaag
    34561 cagtggctca ttcaccgact tatgtcagcc ccttcgagac gtgctacata aaattcgaat
    34621 gacgcacaac aaggtgcctg ccgtccaact tctgatatca gcgtagctat atcaaccatc
    34681 gggcgaaacg tcgagttagg caccgcctta ttccataaca aagccgggta attcccggct
    34741 ttgttgtatc tgaacttccc ctcggttagc atcaggctat tcgcgtctga cgagagtaac
    34801 accttgaaac gctggcccgt ttttccccgc tcattacgac aactggtaat gctggcattt
    34861 ttgctaattc tactacccct gttggtgctg gcatggcaag cctggcaaag cctgaatgcg
    34921 cttagcgatc aggcggcgct ggttaaccgc actacgctta ttgatgcccg gcgtagtgaa
    34981 gcgatgacca acgcggcgct ggagatggag cgtagctacc gtcagtattg cgtgctggac
    35041 gaccctacgc ttgcgaaggt ttatcaaagc cagcgcaagc gttacagcga aatgctcgat
    35101 gcccacgcag gcgtactgcc agacgataaa ctttaccagg cattgcgtca ggatttgaac
    35161 aatctggccc aacttcagtg taacaacagt ggtcccgatg ctgccgccgc cgcgcgtctt
    35221 gaagcctttg ccagtgccaa taccgaaatg gtgcaggcca cgcgcacagt ggtgttctct
    35281 cgtgggcagc aacttcagcg tgaaatcgcc gaacgtgggc aatattttgg ttggcaatcg
    35341 ctggtgctat ttctggtgag tctggtgatg gtgctacttt tcacgcggat gattatcggg
    35401 ccggtgaaaa atatcgagcg catgatcaac cgtctggggg aagggcgttc tctgggcaat
    35461 agcatttcgt tctgtggacc gagcgagtta cgctcggttg ggcaacgtat tctttggtta
    35521 agtgagcgcc tgtcatggct ggaatcccaa cgccatcaat ttttaagaca tttatctcat
    35581 gaattaaaaa cgccactggc gagtatgcgc gagggcactg aattactggc tgatcaggtt
    35641 gtcgggccgc ttacgccaga gcaaaaagag gtggtgagca ttcttgatag cagcagccgc
    35701 aatttgcaaa aactgatcga acaactgctt gattacaacc gtaaacaggc ggacagtgcg
    35761 gtggaactgg agaatgttga attagcaccg ctggtggaga cagtggtttc tgctcatagc
    35821 ctgcccgcac gggctaaaat gatgcatacc gacgtcgatc tcaaagcaac agcttgcctg
    35881 gcggagccaa tgctgctgat gagcgtactg gataatcttt actccaatgc ggtgcactac
    35941 ggggctgaat ccggtaacat ttgccttcgc agcagtttac atggtgcgcg ggtttatatt
    36001 gatgtcatca atacaggcac gcccattccg caagaggaac gcgccatgat cttcgaaccc
    36061 ttttttcagg gaagccacca gcgaaaaggg gcggtgaagg gcagcggtct gggattaagc
    36121 attgccaggg attgtattcg ccgtatgcaa ggggaactgt atctggtcga cgagagcggg
    36181 caagacgttt gtttccgcat tgaattaccg tcgtcgaaaa acacgaaata atgctaacag
    36241 cctatcctat caggctattt tatttgtcag ttcggccccg ggtagtgctc gaaatcttca
    36301 cgtacgatat gtacgctccg gtttctccgc gctgtccatg tccagactgt ctgcaacaat
    36361 tacgcccact gggataggct ttaagtctgg tgaatatgcg acacattttt caacgattat
    36421 tgccccgacg gctgtggctg gcagggctgc catgtctggc attgctgggt tgcgtgcaga
    36481 atcacaataa gccagccatt gatacgccag cagaagaaaa aattccggtt tatcaactgg
    36541 ctgactatct ttctactgaa tgtagcgaca tctgggcgct gcaaggtaaa tcaaccgaaa
    36601 ccaatccgct ttactggctg cgggcgatgg attgtgctga tcgtttaatg cctgcgcagt
    36661 cgcgtcagca agctcgccaa tatgacgacg gcagctggca aaataccttt aaacaaggga
    36721 ttttgctcgc cgacgccaaa attacgccgt atgagcgccg ccagctggtc gcgcgtattg
    36781 aagcgttaag taccgagatc ccggcacagg ttcgcccact gtatcaactc tggcgcgacg
    36841 gtcaggcgct gcaactgcaa ctggcggaag agcgccaacg ttacagtaaa ctccagcagt
    36901 ccagtgacag cgagctggat acattgcgcc agcaacatca cgttctacag cagcaactgg
    36961 aactcaccac ccgcaagctg gaaaacctga ccgatattga acgccagctc tcgacccgca
    37021 aaccggctgg aaatttctcg ccggatacgc cgcacgagag tgaaaagccc gcgccatcca
    37081 cccatgaggt cactcctgat gagccataaa cctgcgcatt tattattggt cgatgacgat
    37141 ccgggattgc tgaaactgct tggcctgcgc ctgaccagcg aaggctacag tgtggtcacg
    37201 gcggaaagtg gcgctgaagg attacgggta ctgaatcgcg aaaaagtaga tttagttatc
    37261 agcgacctgc ggatggatga aatggacggt atgcagctat ttgctgaaat ccagaaagtg
    37321 cagccgggaa tgccagtaat tattcttact gcgcatggtt ctatccccga tgccgttgct
    37381 gcaacacagc agggcgtttt tagtttcctc accaagcctg tcgataaaga tgcgctatat
    37441 caggcaattg acgatgcgct ggagcaatcc gcgccagcca ccgatgaacg ctggcgcgag
    37501 gcaattgtca cccgcagccc gctgatgctg cgtttgctgg aacaggcgcg gctggtggcg
    37561 caatcagacg tcagcgtttt gattaacggt cagagcggca ccgggaaaga gattttcgcc
    37621 caggctatcc acaacgccag cccgcgcaac agcaaaccat ttattgctat taactgtggc
    37681 gcattgcccg agcaattgct ggagtcggag ctgtttggtc atgcgcgtgg cgcgtttact
    37741 ggcgctgtca gcaatcgcga aggtttattc caggcggcgg aaggcggaac gctatttctc
    37801 gatgaaattg gcgatatgcc tgcgccgtta caggtcaaac tgctgcgcgt gttgcaggag
    37861 cgtaaagtgc gcccgctggg cagtaaccgc gatattgata tcaatgtgcg gattatttct
    37921 gccactcacc gtgatctgtc aaaagcgatg gcgcgcgggg aattccgcga agatctctat
    37981 taccgcctca acgttgtcag cctgaaaatt ccggcactgg cggagcgcac agaagacatt
    38041 ccgctactgg caaatcacct gttgcgccag gcggcagagc gacataaacc gtttgtccgc
    38101 gcgttctcta ccgatgcgat gaaacgcctg atgaccgcga gctggccggg taatgtgcgc
    38161 cagttggtca acgtgattga acagtgcgtg gcgctgacct catctccggt gattagtgat
    38221 gcgctggtgg agcaggcgct ggagggtgaa aatacggcgc tgccaacttt tgttgaggca
    38281 cgtaatcatt ttgaactcaa ctatttgcgt aagctgctgc aaatcaccaa aggcaacgtc
    38341 acccacgcgg cgagaatggc ggggcgcaac cggacagaat tttataaact gctttcccga
    38401 cacgagctgg atgcaaacga tttcaaggaa tgaattggcg ttatgtgtta cgtttagcag
    38461 atcaaaagac aggcgacctt ttcaaggaat agcatgaaaa agattgatgc gattataaaa
    38521 cccttcaagc tggacgatgt ccgcgaagca ctggccgaag tcggtattac cggcatgacg
    38581 gtgaccgaag tgaaaggctt tggtcgccag aaaggccata ccgagctgta ccgcggcgcg
    38641 gagtatatgg tggattttct gccgaaagtg aaaattgaga ttgtcgtacc ggacgacatt
    38701 gtcgatacct gtgtcgatac cattattcgc acggcgcaaa ccggcaaaat cggtgacggt
    38761 aaaatcttcg tctttgacgt ggctcgggtc attcgcatcc gtaccggtga agaggacgac
    38821 gcggcaattt aaaccgcgtc aagggttgcc ggatgtttcc atccggcaac atcaaattac
    38881 agcaccttat gcgggccaaa gcattcgtaa tgaatgtttt cctgcttcac gcccagatcc
    38941 actaactgtt tcgcggtaaa ctgcatgaag ccaaccgggc cgcagagata gaactgcatt
    39001 gtcggatcgc tgaacgcacc ttccagtttg ctcaaatcca tcagaccttc gctatcaaac
    39061 tgacctttag cgcgatcggc ttcgctcggc tgacgatacc aggtgtgcgc ggtaaagcgc
    39121 ggcagtgact gccccagttc cttaacttca tcagcaaagg cgtgaacttc gccattttct
    39181 gccgcatgga accagttcac ttgtgctgtg tggcctgctt ttgccagcgt gtcgagcatt
    39241 gccagcattg gcgtttgacc aacaccggta gagattaacg tcactggtgt gtcatctgcg
    39301 acagccataa agaaatcacc tgccggagcg accagtttca cgacatcgcc aacattggcg
    39361 tgattgtgca accagttgga tacctgccca ccctcttcgc gtttcaccgc aatacgatag
    39421 cctttgccat ccggtttgcg agtcaaagag tactgacgaa tttcctgatg cgggaaacct
    39481 tccggcttca gccagacgcc gagatattgc cccggacggt attctgccac tgcgccaccg
    39541 tcgaccggct ccagttcgaa gctggtgata agcgcgctgc gcggtgtttt agccacaatg
    39601 cggaaatcgc gagtaccttc ccaaccaccg gctttgctgg cgttttcgtt atagatttcc
    39661 gcctcgcgat tgataaatac attagccagt acaccatagg ctttacccca cgcgtccagc
    39721 acttcctgcc ccgggctgaa catttcgtcc agcgttgcca acaggtgttc accgacgatg
    39781 ttgtactgtt ccggtttgat ctggaagctg gtgtgcttct gcgcgatttt ttctaccgct
    39841 ggcagcagcg caggcaggtt ttcaatatta ctggcgtagg cggcaatagc gttaaacagg
    39901 gcttcacgtt gatcgccatt acgctggtta ctcatgttaa aaatttcttt gagttctggg
    39961 ttatgagtaa acatacggtc gtagaaatgg gcggttaact ttggccccgt ttccaccagt
    40021 aaagggatgg tggcttttac tgtagcgatg gtttgagcgt caagcatatg gtcttccttt
    40081 tttgcatctt aattgatgta tctcaaatgc atcttataaa aaatagccct gcaatgtaaa
    40141 tggttctttg gtgtttttca gaaagaatgt gatgaagtga aaaatttgca tcacaaacct
    40201 gaaaagaaat ccgtttccgg ttgcaagctc tttattctcc aaagccttgc gtagcctgaa
    40261 ggtaatcgtt tgcgtaaatt cctttgtcaa gacctgttat cgcacaatga ttcggttata
    40321 ctgttcgccg ttgtccaaca ggaccgccta taaaggccaa aaattttatt gttagctgag
    40381 tcaggagatg cggatgttaa agcgtgaaat gaacattgcc gattatgatg ccgaactgtg
    40441 gcaggctatg gagcaggaaa aagtacgtca ggaagagcac atcgaactga tcgcctccga
    40501 aaactacacc agcccgcgcg taatgcaggc gcagggttct cagctgacca acaaatatgc
    40561 tgaaggttat ccgggcaaac gctactacgg cggttgcgag tatgttgata tcgttgaaca
    40621 actggcgatc gatcgtgcga aagaactgtt cggcgctgac tacgctaacg tccagccgca
    40681 ctccggctcc caggctaact ttgcggtcta caccgcgctg ctggaaccag gtgataccgt
    40741 tctgggtatg aacttggcgc atggcggtca cctgactcac ggttctccgg ttaacttctc
    40801 cggtaaactg tacaacatcg ttccttacgg tatcgatgct accggtcata tcgattacgc
    40861 cgatctggaa aaacaagcca aagaacacaa gccgaaaatg attatcggtg gtttctctgc
    40921 atattccggc gtggttgact gggcgaaaat gcgtgaaatc gctgacagca tcggtgctta
    40981 cctgttcgtt gatatggcgc acgttgcggg cctggttgct gctggcgtct acccgaaccc
    41041 ggttcctcat gctcacgttg ttactaccac cactcacaaa accctggcgg gtccgcgcgg
    41101 cggcctgatc ctggcgaaag gtggtagcga agagctgtac aaaaaactga actctgccgt
    41161 tttccctggt ggtcagggcg gtccgttgat gcacgtaatc gccggtaaag cggttgctct
    41221 gaaagaagcg atggagcctg agttcaaaac ttaccagcag caggtcgcga aaaacgctaa
    41281 agcgatggta gaagtgttcc tcgagcgcgg ctacaaagtg gtttccggcg gcactgataa
    41341 ccacctgttc ctggttgatc tggttgataa aaacctgacc ggtaaagaag cagacgccgc
    41401 tctgggccgt gctaacatca ccgtcaacaa aaacagcgta ccgaacgatc cgaagagccc
    41461 gtttgtgacc tccggtattc gtgtaggtac tccggcgatt acccgtcgcg gctttaaaga
    41521 agccgaagcg aaagaactgg ctggctggat gtgtgacgtg ctggacagca tcaatgatga
    41581 agccgttatc gagcgcatca aaggtaaagt tctcgacatc tgcgcacgtt acccggttta
    41641 cgcataagcg aaacggtgat ttgctgacaa tgtgctcgtt gttcatgttg gatgcggcat
    41701 gaacaccttc ttaggcctac aaaatcttgc aaattcaata tattgcaatc tccgtgtagg
    41761 cctgataagc gtagcgcatc aggcaatttt tcgtttatga tcatcaaggc ttccttcggg
    41821 aagcctttct acgttatcgc gccatcaaat ctgtcgtaac tgcgcctcaa catacaaata
    41881 gccaattccc agcacctgtt gtgcgcggct taattgcccg aagccaattt gcgtcgcttt
    41941 cacgctcggt ccttcgtcgc ggtcgaacgg gttaaatcct gtctggcgaa taatagtatt
    42001 aagccagttc tcaccaaagg cacaactgcg accgtacaac caaatctgat taatattgag
    42061 gatgttgagg aagttataca gactcaaacc aatggcattg gcagagcgat caacccagct
    42121 ggtgatccac agttctccac tctgccaggc agcgattaac tgcgcggttg tcagtttttc
    42181 aggatcaagc tgagtattaa ccggttgtga ttttagccat acccgcgcct gtttttttaa
    42241 tgcacttaac gaggcgacgg tttccaggca gccgtaacgt ccacagtcgc agacgacgcc
    42301 atccggatta acgatggtgt gaccaatctg tccgctacca tacaaactgc cgcgataaat
    42361 ttgctcgtta atcacgaacg acgagccaat gccgtaatca acgttgatca cgcagaaatc
    42421 ccgttcctgc gaattatttt gccatttctc cgccagcgcc agcatcacgc agtcattatc
    42481 gaccagcacc cgaatgccca gcttctcttc cagcagatac ttcacctcaa ctggcgttgt
    42541 ccacggcgct tgcggcatgg tttgcgatac gccggtcacc ggatcaacct gcccgtggat
    42601 tgccagcgcc agattgatgg tgcggtccgg ccacaattta cggtggcgat gccagcattt
    42661 ttcgatttcg gacagcagcg cctgcggcgt cggtacatca atctgtaaat actcaaattc
    42721 gcctttcgga cttaaacaag cattagcaac ctgacactca atactggtgg gcgtcacgtt
    42781 caggcacagc gtccagtcac cttccggcgc aatcagccat gtaccgctac tatgcccgcg
    42841 cgtctggctt tcatcgtcaa tattcaccac ccgtttttcg ctttccagtt cctgcaaaat
    42901 attactgact gccggaatcg aaatttgcgc cagccgggcc agcgttgatt tattggcgcg
    42961 cttttgccgg tacagcagtt ccagaatcac gcatttgtta tggtggcgaa tctgttgatt
    43021 gttaatgcag gctctcatct tcactaaacc ttgtttagtt aattgcgtga ttattccgcg
    43081 aaaacattgc cagattgttc aatacactgc cacaaatctt ttaattcagt atgtcttgtt
    43141 aatattgagg gcaccatgac tccagtaaaa gtgtggcaag agcgcgttga gatcccgacc
    43201 tatgaaaccg ggccgcagga tatacatccc atgttcctgg aaaatcgcgt ttatcaggga
    43261 tcgtccggcg cggtttatcc ctacggcgtg accgatacgc tgagcgagca gaaaaccctg
    43321 aaatcctagc aggcggtgtg gctggaaaac gactacatca aagtgatgat cctgccggaa
    43381 ctgggcggtc gggtgcatcg cgcatgggat aaagtgaaac agcgcgattt tgtctatcac
    43441 aatgaagtca ttaaacctgc gctggtgggg ctgctgggac cgtggatctc cggcgggatt
    43501 gagtttaact ggccgcaaca ccatcgcccg accaccttta tgcccgttga tttcaccctc
    43561 gaagcccatg aagacggtgc acagacggtg tgggtcggcg aaacggagcc gatgcatggt
    43621 ttacaggtga tgacaggttt caccctgcgc cctgaccggg cggcactgga aatcgccagc
    43681 cgcgtctata acggcaacgc cacgccgcgt catttcttgt ggtgggccaa cccggcagtg
    43741 aaagggggtg aagggcatca gagcgttttc ccaccggatg tgactgcggt gtttgatcac
    43801 ggcaaacggg ccgtctccgc tttccccatc gccacttact acaaagtgga ctactccgcc
    43861 ggagtggaca tttctcgcta taaaaatgtg cctgttccaa cctcatatat ggccgaaaaa
    43921 tcgcagtacg attttgtcgg cgcgtggtgt cacgatgaag atggcggttt gctgcacgtt
    43981 gccaaccacc atattgcgcc aggtaaaaaa cagtggagtt ggggacacag tgaatttggc
    44041 caggcgtggg ataagagtct gaccgacaat aacggcccgt atatcgaact gatgaccggt
    44101 atttttgccg ataaccagcc tgattttacc tggcttgatg cttacgaaga gaagcgtttc
    44161 gagcagtatt tcctgcctta tcattctctg ggcatggtgc aaaatgcctc ccgcgatgcg
    44221 gtgataaaac tccagcgtag tgagcggggg attgagtggg ggctgtatgc catctctccg
    44281 ttgaacggat accgtctggc gatccgcgaa atcggcaaat gcaacgcgtt gctcgatgat
    44341 gccgtggccc tgacaccagc gaccgccatc cagggcgtgt tgcacggtat caatcctgac
    44401 agactgacca ttgaactctc cgatgccgac ggcaatattg tactgagtta tcaggaacat
    44461 cagccgcagg agttgccgtt gccggacgtc gccaaagcgc cactggcagc acaagacatt
    44521 accagtacag atgaagcctg gtttatcggt cagcatctgg agcaatatca tcacgccagc
    44581 cgttcaccgt tcgattacta cctgcgcggc gtggcgctgg acccgctgga ttatcgctgt
    44641 aacctggcgc tggcgatgct ggaatataac cgcgcagatt tcccgcaagc ggtggcgtat
    44701 gccagtcagg cgctgaaacg cgcacatgcg ctgaacaaaa acccgcagtg tggacaagca
    44761 agtttgattc gcgccagtgc ttacgaacgt cagggacaat atcaacaagc cgaagaggat
    44821 ttctggcgtg cggtctggag cggcaatagt aaagcaggcg gctattatgg tctggcacga
    44881 ctggcggcgc gtaatgataa cttcgacgcg ggtctggatt tttgccaaca aagtcttcgt
    44941 gcctgcccaa ccaatcagga agtgctttgc ttgcataatc tgctgctggt gttaagtggt
    45001 cgtcaggaca acgcgcgttt gcagcgcgag aaactgctgc gtgattatcc gctgaacgcc
    45061 actctgtggt ggctgaactg gttcgatggt cgtagcgaat cagccctcgc gcagtggcgc
    45121 ggtctgtgtc agggacgcga cgttaacgct ctgatgaccg ccgggcaact gattaactgg
    45181 ggaatgccca ccctggcggc agagatgctg aacgcactgg actgccagcg cacgctgccg
    45241 ctttacctgc aagccagctt gctgccgaaa gccgaacgtg gcgaactggt cgcaaaagcc
    45301 attgatgtct tcccgcagtt tgtccgtttc ccgaatacgc tggaagaagt ggcggcgctg
    45361 gagagtattg aagagtgctg gtttgctcgc catttactgg cctgcttcta ctacaacaaa
    45421 cgtagctaca acaaagccat tgccttatgg caacgttgcg tagagatgtc gccggagttt
    45481 gccgacggct ggcgcgggtt agcgatccat gcgtggaata agaaacacga ttatgagctg
    45541 gccgcgcgtt atcttgataa tgcttatcag cttgcgccgc aggatgcacg tctgcttttc
    45601 gaacgagatt tgctggataa gttaagtgga gccacaccgg agaaacgact ggcgcgtctg
    45661 gaaaataatc tggaaattgc gctgaaacgc gacgacatga ccgcagaact gctcaatttg
    45721 tggcatctca cgggtcaggc agacaaagcg gcggacattc tcgccacgcg taaattccac
    45781 ccgtgggaag gcggggaagg gaaggtcacc agtcagttta tcctcaacca gttattacgc
    45841 gcctggcagc atcttgatgc cagagagccg cagcaggcca gcgaactgct tcatgccgcg
    45901 ctgcattatc cggagaattt aagcgaaggc cgtttaccgg ggcaaactga taacgacatc
    45961 tggttctggc aggcgatatg cgccaacgcg cagggcgatg aaactgaagc gatgcgttgt
    46021 ttacgtctgg cggcgaccgg cgatcgcacc attaacatcc acagttatta caacgatcag
    46081 ccggttgatt atctcttctg gcaaggaatg gcgctgcgac tgctgggtga acagcaaacc
    46141 gcacagcaac tgtttagtga aatgaaacag tgggcgcaag agatggcgaa aaccagtatt
    46201 gaggcggatt tctttgctgt ttcacaacct gacctgttat cgctgtatgg cgatttacaa
    46261 cagcagcata aagaaaaatg cctgatggtg gcgatgctgg cgtccgcggg actcggggag
    46321 gttgcgcaat atgagtctgc tcgcgctgaa ttgacggcga ttaatccggc ctggccgaaa
    46381 gcggcattat tcaccactgt gatgcctttt atttttaact acgttcacta aaccctatta
    46441 actgtaatat ttgagcggca cgcgctgccg ctcatcacaa aataattaga ataagccgtt
    46501 gttaataaaa accctacagg aggcactatg cctaaaaaaa tgagaacaac ccgtaatcta
    46561 ttattaatgg ctacgctatt aggtagcgca ctatttgcca gggctgcgga taaagaaatg
    46621 accattggtg caatatacct tgatacccag ggatattacg ctggagtgcg tcagggcgtt
    46681 caggatgcgg caaaagattc ttcagtacag gtacagttaa ttgaaactaa cgcccagggt
    46741 gatatttcga aagaaagtac ctttgttgat accctcgtgg cgcgtaatgt cgatgccatt
    46801 attttatcgg cagtgtctga aaatggcagt agccgtaccg ttcgtcgcgc cagtgaagcg
    46861 ggtattccgg tgatttgcta caacacctgt attaatcaaa agggtgtcga taaatatgtc
    46921 tcggcgtatc tggtcggcga tccactggaa tttggtaaaa aactgggtaa cgctgccgcc
    46981 gattatttta ttgccaataa aattgaccag ccgaaaattg ccgtcatcaa ttgcga
//
LOCUS       DADLSA010000016        46015 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.016, whole genome shotgun sequence.
ACCESSION   DADLSA010000016 DADLSA010000000
VERSION     DADLSA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.016"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            181..647
                     /locus_tag="JN691_000920"
                     /pseudo
     CDS             181..647
                     /locus_tag="JN691_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
     gene            complement(859..1023)
                     /locus_tag="JN691_000921"
     CDS             complement(859..1023)
                     /locus_tag="JN691_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242429.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1046..1747)
                     /locus_tag="JN691_000922"
     CDS             complement(1046..1747)
                     /locus_tag="JN691_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY7242430.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1852..2748
                     /locus_tag="JN691_000923"
     CDS             1852..2748
                     /locus_tag="JN691_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7242431.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            complement(2955..3323)
                     /locus_tag="JN691_000924"
     CDS             complement(2955..3323)
                     /locus_tag="JN691_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY7242432.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(3616..4602)
                     /locus_tag="JN691_000925"
     CDS             complement(3616..4602)
                     /locus_tag="JN691_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY7242433.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(4672..5154)
                     /locus_tag="JN691_000926"
     CDS             complement(4672..5154)
                     /locus_tag="JN691_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY7242434.1"
                     /translation="MILSIDSNDANTAPLHKKTISSLSGAVESMMKKLEDVGVLVAHI
                     LMPILFITAGWGKITAYSGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFGFLTRTTA
                     LFTAGFTLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW
                     "
     gene            complement(5250..5549)
                     /locus_tag="JN691_000927"
     CDS             complement(5250..5549)
                     /locus_tag="JN691_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242435.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(5539..5943)
                     /locus_tag="JN691_000928"
     CDS             complement(5539..5943)
                     /locus_tag="JN691_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242436.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(5946..6251)
                     /locus_tag="JN691_000929"
     CDS             complement(5946..6251)
                     /locus_tag="JN691_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY7242437.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(6289..6657)
                     /locus_tag="JN691_000930"
     CDS             complement(6289..6657)
                     /locus_tag="JN691_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HAY7242438.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(6804..7187)
                     /gene="mzrA"
                     /locus_tag="JN691_000931"
     CDS             complement(6804..7187)
                     /gene="mzrA"
                     /locus_tag="JN691_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAY7242439.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(7191..7853)
                     /locus_tag="JN691_000932"
     CDS             complement(7191..7853)
                     /locus_tag="JN691_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7242440.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEEQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(8198..8974)
                     /gene="exuR"
                     /locus_tag="JN691_000933"
     CDS             complement(8198..8974)
                     /gene="exuR"
                     /locus_tag="JN691_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAY7242441.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            9270..9986
                     /locus_tag="JN691_000934"
     CDS             9270..9986
                     /locus_tag="JN691_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7242442.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAENQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            10016..10516
                     /locus_tag="JN691_000935"
     CDS             10016..10516
                     /locus_tag="JN691_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7242443.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            10608..11414
                     /locus_tag="JN691_000936"
     CDS             10608..11414
                     /locus_tag="JN691_000936"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-fimbriae usher protein"
                     /protein_id="HAY7242444.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNTDVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLN"
     gene            11442..11642
                     /locus_tag="JN691_000937"
     CDS             11442..11642
                     /locus_tag="JN691_000937"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242445.1"
                     /translation="MVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVA
                     LKVYENNQLTRACAVYQNRRSD"
     gene            11611..13287
                     /locus_tag="JN691_000938"
     CDS             11611..13287
                     /locus_tag="JN691_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane fimbrial user protein"
                     /protein_id="HAY7242446.1"
                     /translation="MPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLH
                     PQYELYAGLANADDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSN
                     PGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISATVSLNFVGWNMMLGYTRTQNN
                     TDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDNG
                     GDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSF
                     SHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSST
                     LAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDS
                     VLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNY
                     IGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVV
                     KKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            13740..14372
                     /locus_tag="JN691_000939"
     CDS             13740..14372
                     /locus_tag="JN691_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HAY7242447.1"
                     /translation="MKNLPIGGVWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFP
                     EFAHATPRVQLDLHPTGSVNGSNYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAA
                     GRRDGYFSIYRQGGTTTDEGERIDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRP
                     VVLPGIRYAVMCVPTPLTLAVDKFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(14460..15758)
                     /locus_tag="JN691_000940"
     CDS             complement(14460..15758)
                     /locus_tag="JN691_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7242448.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            16241..17653
                     /gene="uxaC"
                     /locus_tag="JN691_000941"
     CDS             16241..17653
                     /gene="uxaC"
                     /locus_tag="JN691_000941"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAY7242449.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRCPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            17668..19155
                     /locus_tag="JN691_000942"
     CDS             17668..19155
                     /locus_tag="JN691_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAY7242450.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKRELGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            19238..19789
                     /locus_tag="JN691_000943"
     CDS             19238..19789
                     /locus_tag="JN691_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAY7242451.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(19794..21038)
                     /gene="sstT"
                     /locus_tag="JN691_000944"
     CDS             complement(19794..21038)
                     /gene="sstT"
                     /locus_tag="JN691_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAY7242452.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(21634..22599)
                     /gene="alx"
                     /locus_tag="JN691_000945"
     CDS             complement(21634..22599)
                     /gene="alx"
                     /locus_tag="JN691_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAY7242453.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(22870..23886)
                     /locus_tag="JN691_000946"
     CDS             complement(22870..23886)
                     /locus_tag="JN691_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY7242454.1"
                     /translation="MEYPRVMIRFAVIGTNWITRQFVEAAHERGKYKLTAVYSRSLEQ
                     AQHFANDFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPL
                     ASNLAEVDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSS
                     RYQRYLDGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAH
                     GVVVMDYGDFSVTLQHSKVSDSVLGSEIQGEAGALVIEKLSECQKVCFVPRGSQMQDL
                     TQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL
                     YLPK"
     gene            complement(23947..24639)
                     /locus_tag="JN691_000947"
     CDS             complement(23947..24639)
                     /locus_tag="JN691_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAY7242455.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGHKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(24716..25219)
                     /locus_tag="JN691_000948"
     CDS             complement(24716..25219)
                     /locus_tag="JN691_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7242456.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            25304..26440
                     /gene="rlmG"
                     /locus_tag="JN691_000949"
     CDS             25304..26440
                     /gene="rlmG"
                     /locus_tag="JN691_000949"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAY7242457.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKVVKLGRRR"
     gene            26725..27039
                     /locus_tag="JN691_000950"
     CDS             26725..27039
                     /locus_tag="JN691_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HAY7242458.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKE
                     KK"
     gene            27036..27452
                     /gene="higA"
                     /locus_tag="JN691_000951"
     CDS             27036..27452
                     /gene="higA"
                     /locus_tag="JN691_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAY7242459.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(27497..29515)
                     /locus_tag="JN691_000952"
     CDS             complement(27497..29515)
                     /locus_tag="JN691_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAY7242460.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKITLQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(29741..32092)
                     /gene="ygjK"
                     /locus_tag="JN691_000953"
                     /pseudo
     CDS             complement(29741..32092)
                     /gene="ygjK"
                     /locus_tag="JN691_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(32109..33180)
                     /gene="ygjJ"
                     /locus_tag="JN691_000954"
                     /pseudo
     CDS             complement(32109..33180)
                     /gene="ygjJ"
                     /locus_tag="JN691_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(33314..34747)
                     /locus_tag="JN691_000955"
     CDS             complement(33314..34747)
                     /locus_tag="JN691_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7242461.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(34810..35259)
                     /locus_tag="JN691_000956"
     CDS             complement(34810..35259)
                     /locus_tag="JN691_000956"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAY7242462.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(35256..38348)
                     /gene="ebgA"
                     /locus_tag="JN691_000957"
     CDS             complement(35256..38348)
                     /gene="ebgA"
                     /locus_tag="JN691_000957"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HAY7242463.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSH
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(38532..39515)
                     /gene="ebgR"
                     /locus_tag="JN691_000958"
     CDS             complement(38532..39515)
                     /gene="ebgR"
                     /locus_tag="JN691_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAY7242464.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            39734..40065
                     /locus_tag="JN691_000959"
                     /pseudo
     CDS             39734..40065
                     /locus_tag="JN691_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(40107..41597)
                     /gene="ygjG"
                     /locus_tag="JN691_000960"
     CDS             complement(40107..41597)
                     /gene="ygjG"
                     /locus_tag="JN691_000960"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAY7242465.1"
                     /translation="MITAFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     PAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            41904..43424
                     /locus_tag="JN691_000961"
     CDS             41904..43424
                     /locus_tag="JN691_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY7242466.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAILWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVAIMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQFGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(43578..44201)
                     /locus_tag="JN691_000962"
     CDS             complement(43578..44201)
                     /locus_tag="JN691_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY7242467.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            44478..45242
                     /gene="nfeF"
                     /locus_tag="JN691_000963"
     CDS             44478..45242
                     /gene="nfeF"
                     /locus_tag="JN691_000963"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HAY7242468.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEKYDPQRVRAAAYWHAK"
     gene            complement(45296..45371)
                     /locus_tag="JN691_000964"
     tRNA            complement(45296..45371)
                     /locus_tag="JN691_000964"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45336..45338),aa:Ile,seq:cat)
     gene            45496..46002
                     /gene="mug"
                     /locus_tag="JN691_000965"
     CDS             45496..46002
                     /gene="mug"
                     /locus_tag="JN691_000965"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HAY7242469.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT        11372 a        11626 c        11930 g        11087 t
ORIGIN      
        1 aagtgggggc tgcatattgg cgcgacgctg gaaaaacagt gcgagcgcgg tttgctggcg
       61 aaagatctct cttcatccat tgtgattcgt accagcgcgg catccgatgc gcgtatgggc
      121 ggcgctacgc ttccggctat gagtaactcc ggctcgggta accaggggat caccgcaaca
      181 atgcctgtgg tggttgtagc agaacacttc ggagcggatg atgaacgact ggcgcgtgcg
      241 ctgatgcttt ctcatttgag cgcaatttac atccataacc agttaccgcg tttgtctgca
      301 ctttgtgccg caacgaccgc agcaatgggg ccgccgccgg gatggcatgg ctggtggatg
      361 ggcgttatga aactatctcg atggcgatca gcagtatgat cggcgatgtc agcggcatga
      421 tttgcgatgg tgcgtcgaac agctgcgcga tgaaggtttc gaccagtgct tcggctgcgt
      481 ggaaagcggt gttaatggcg ctggatgata ccgccgtgac cggcaatgaa gggatcgtgg
      541 cgcatgatgt tgagcagtcg attgccaacc tgtgtgcgtt agcaagccat tcgatgcagc
      601 aaacggatcg gcagattatc gagattatgg cgagcaaggc cagataaacg ccaggttttg
      661 agtattgtgc cggatgcgat gctggcgcac cttatccggc ctacggggtg ctcaacttgt
      721 tgatgttgtt gtgttttgta cctgatgccg gatgcgatgc tggcgcatct tatccggcct
      781 acggggtgtt taatttgttg atgttgttgt gttttgtagg ccggataagg cgtttacgcc
      841 gcatccggca acctgtcttt acgcggtagc ttcatcctcg gctaaacgcg tttcagcatc
      901 cgcgacgata gcttccagct cgctcaacat ctcttcatag ccaaaatttt tggcggtgcg
      961 agcaggttct ttcgccacca cgggcttcgc tggctggcgc tttttggtcg attttttact
     1021 catcatcatc tccctgtaaa aacaactaga caggcaaatc tatcagcaaa gcgcgcagtg
     1081 gggaatcggc aaccagcgta atgttagcct cgtcacgaat aaacgcccca tcaccgcagg
     1141 ttagcgccgc tttctcttca tgatgcgtaa gcgcatgaaa tttcccgtga atcgattgca
     1201 aatacgcgcg tggcccatgc aactggaaat tcgcactttc gcctttgtcg agcacgatat
     1261 ggtgcagcca cacttgttgg cgtaattgca ggcttcccat cgcaccctct ggcgaggcga
     1321 ttaattgctg cttgtccata ttaagcgcca gcttttgaat cagcggattc tctcgctgcg
     1381 ggcaggtatc cagccaaagc tgcattcgcg ttaacggttt gtctttgctg agattgtgtt
     1441 cgctatagct gacacccggc tgggtagaga gcagcaacgc ctctccggcg ctggcctgaa
     1501 catgattgcc ttcgctgtcg cgatactctg cttctccatc cagaatcaca tttaaaatat
     1561 cgactttggg ataggttcgc ggctgaaagg cggcacctgg ggccagcact tcctggttaa
     1621 gcacacgcag ggaggcatag cctaacaatt tcgggtcgaa gtagtgtcca aaggaaaaag
     1681 tataccgggc ctgcaaccat ccgtagtctg cttgtccaca ctgcctggca gttcgggtag
     1741 taatcataat tcttgacctc tctttacttc cttttatggt aaggggctgg acgctacatt
     1801 gttagccaga tattctgccc ggtatgttca aatttcctga atgagaacga aatggccaaa
     1861 gaaagggcat taacgctgga agcactacgg gttatggatg cgatcgatcg ccggggcagt
     1921 tttgcggcgg cagcggatga gctgggacgc gtgccttccg cacttagcta caccatgcaa
     1981 aaactggaag aagagctgga tgtggtgctg tttgaccgct cgggccatcg caccaaattc
     2041 accaatgtcg ggaggatgtt gctggagcga gggcgcgttt tgctggaagc cgcagataaa
     2101 ctgactactg atgcggaagc tctcgcgcgc ggttgggaaa cgcatctcac cattgtgacc
     2161 gaagcgctgg taccgacacc tgcctttttc ccgttaatcg acaaactggc ggcaaaagcc
     2221 aatacccaac tggcaatcat cacagaagtg ctggcggggg cgtgggaacg gctggagcag
     2281 gggcgggcgg atatcgttat cgcgccggat atgcattttc gttcctcgtc ggagatcaac
     2341 tcgcgcaagc tctatacgtt aatgaacgtc tacgttgccg cgcctgatca cccgattcat
     2401 caggagccgg aaccgttatc tgaagtgacg cgtgtgaaat atcgtggaat tgcggtagcg
     2461 gataccgctc gtgagcgccc ggtgttgacc gtacagctgc tggacaaaca gccgcgctta
     2521 acggtgagca cgattgaaga taaacgtcag gcattactgg cggggcttgg cgtggcgacg
     2581 atgccgtatc cgatggtcga aaaagatatt gcggaagggc ggttgcgtgt cgtcagcccg
     2641 gaatcgacca gcgagatcga tattattatg gcctggcgtc gtgacagtat gggggaagcg
     2701 aaatcctggt gtctgcggga aattccaaag ttgttcgctg gaaaataatc tattaagcac
     2761 ctttcacaag gtgcttctga ctgctgacaa acgcaaaact gcctgatgcg ctacgcttat
     2821 caggcctacg cagtcactgc aatatattga attttggcaa tttgtaggct ggataaggcg
     2881 tttacgccgc atccggcata aacaaagcgc actttgtcaa caatttgaag caccttttac
     2941 aaggtgctta atcattattc ttgttccagt tttggatctg gtccaaagcg gttttcgcct
     3001 ggcgtacccg cctggcagtt aaagacgatg ataataagcc agccgataaa cgggattaag
     3061 aatagcagtg cccaccacgc cgagcggtcg gtgtcgtgca ggcggcggaa ctgaaccgcc
     3121 caccacggta aaaacaccag aataccgtag attgtcgtca ggatgccttc gccaccagcg
     3181 cgttgccagc ctaacatttt atccagcagc cccagcacga acgtaaagat gatgttgacc
     3241 agaataaaca tccagtactc tttgcgccgc gcacgacccc ggaaaccgac ataatttttt
     3301 agtactttca gataccagtc catttttgcc tcaattttgc tcaatcactt gtttttaaag
     3361 cagcaaatta agaatagacg tgaatgaaat ggcagtaaat attcgttcaa ggaattaata
     3421 aagtgccgtg aaggcgcgct aagactgatg gcaagcgcaa aatcgccaga tgcgctacgc
     3481 ttatctggcc tacgcggtca ttgtaacata ttgaatataa acgctttgta ggtttgataa
     3541 ggcgttcacg ccgcatccga catgaacaac atgcacttag ctaattatca gaggcacctc
     3601 gtaaggtgcc tttttttaac cgaagcgaac atctcgtcca tgcggttcat cgagatcctg
     3661 ccacggacca attgaaataa tccccgtagg gttgatggtc ttatggctgc ggaagtaatg
     3721 attacggatg tgatcgaaat tgacagtttc ggcgatcccc ggcatctggt agatatcgcg
     3781 caggaagcca tacagattca ggtaatcgct gatgcggtgc ttatcacact tgaagtgggt
     3841 cacatacact ggatcaaaac gcaccagcgt agtccacagg cgaatatcgg cttcggttag
     3901 ctggttgccg gtcaggtaac gatgctggcc taaaatctgc tccagtcgcg ccagcgattc
     3961 aaacactttc gccaccgcct cgtcgtaggc ttgctggctg gtggcaaaac cagctttata
     4021 cacgccgttg ttaacggtgt cataaatcca gccgttaagt tcgtcaattt tcgtttgcag
     4081 ggctggtggg tagtaatcac ccgcttttgc gcccagcgca tcaaacgcgg tattgaacat
     4141 gcggatgatt tctgctgatt cgttgctgac gatggtgtgg ttctttttgt cccacagcac
     4201 gggaacagta actcgtccgc tgtagtgtgg atcggcgtgg agataaagct gatacagaaa
     4261 ttcatgttga tagagcgtat ctccggttgc tcccggaaaa ctgtcatcaa aggtccagcc
     4321 gttttccagc atcagcgggt tcactacgga aacggaaata aacggttcca gtcctttgag
     4381 tttgcgcatg atcagcgtgc ggtgcgccca cgggcaggcg agtgaaacat agagatgata
     4441 acgatctttc tctgcgataa aaccgcctgt gccagtgggg ccaggtgcgc catcggcagt
     4501 gagccagtta cgaaatgcgg aagctgaacg ttgaaattta ccgccggtag atttggtatc
     4561 gtaccaggtg tcatgccaga cgccgtcaat cagttgaccc atttttctct cctccaatag
     4621 caaaaacgag gagcttcctc ctcgtttttt atcgttaagt atagcgcgtg cttaccactt
     4681 tttattcagc aggcggtcga tgctatacgc gcccggaccg gtaattgcca gcagcaggaa
     4741 tccgccagaa attgtcaggt ttttcatgaa catcagcgag ttgacgcctt cagcaaagtt
     4801 gctgtgaaat aaaaatgccg tcagcagcgt aaagcccgca gtaaacaggg ctgtggtgcg
     4861 agtcaggaaa ccgaacagga ttgccagacc accaccgaac tcaagcagaa tcaccagcgg
     4921 cagcataaaa cccgggacac ccattgcttc catatattgt tgggtaccag agtaagcggt
     4981 gatttttccc cagcctgcgg taataaacag aatcggcatt aaaatgtgcg ctaccagtac
     5041 accaacatct tctaattttt tcatcatact ctccactgcg ccactcaggc tgctgattgt
     5101 ttttttgtgc aagggcgcgg tattagcgtc gttgctgtcg atggagagaa tcataaacgt
     5161 ggtgaatgat gattgttagc aaggaaaact gtcaaaaata ttcaaaaaat ttgagggata
     5221 aggccggaat tgctccggcc agagggaagt taaccgcgaa gctgttgctg cttgagggtc
     5281 gttttaacca gacgccaggc gctccatacg ccaaaaccgc gtctggccca gcggaccagc
     5341 atattaggat ggcgaatcgt ccagatcgcc atcacgctac tgccaaccag cgcccaggag
     5401 cgcagactta gcagcatatt ccagcgacga tcgtaagcgc ctgttgcctc cagccattca
     5461 cgacgactgg cggaaagatc cagccgttgt tgctggatct ggctaagcag ttgcgcctta
     5521 cgtcgttcac gttcgacttt actgctcacg ggactcctcc tcgagcagtt gccgatcgtt
     5581 tgccagctca tggcgtgtat ggcgcagcaa ggtggattta cgcgatttac gtaacgtcca
     5641 gataccgcca atcagtgcca gtagcagcaa caccacggtg gtggcaatca tcgcattcag
     5701 gcgatattgc ggatcaaccg cccaaataat tagcaccatc aggctcataa gaccaaatgc
     5761 agcgaaaagc atcgtcaggc ccagcatcag taaaagttga aagagattag ctttttcctc
     5821 ttccagctcc accaccgcca gccgcagacg tgtttccacc atttcaacca tgatggaaac
     5881 aattcgctgc ccgatgccca gaacgctttt accgggccct tgcgcgtgat gagtgtccgc
     5941 cataattaac gacgcgacag cagaacgccg agcactacac cgattgcagc gccaattccc
     6001 acgccggtcc acggattttc acgcacatac tcatcagcac gcgccgccgc gacacgggtt
     6061 tgtttggcga tggcatcacc ggtttcaccc aggcgataac ggctctgttt cagtgcctgc
     6121 tccgctttgc tacgaatctt actcaactct tctttcgact tctcgccaga tgagctaagc
     6181 acctcttcca gcgtatcgga aagggatttc aactcagcac gcagatgttc cgtagtgtgt
     6241 tctttcgaca tagttttctc caggtgagta agtagtgact attgtgagtt agtagtcgcg
     6301 cgcttccagc tttttcagct cttcctgcgc ttctgccagt ttccgttcgc gtttggcaat
     6361 cttatcggca tcgccttttt gcttcgcctc ggctaaatct tgctggcgtt ccgccacctc
     6421 atctttctgc tttgcgattt tcttgtgatg atcggcacgc agctggctat ctgaacagtt
     6481 ggcccggact tcactcaggg ctttattcag accgtcaata cgattctggt tttggtgttt
     6541 ttcggcatag ctgatctcct taaggatatt ttgctccttt tcctgacaca gggtagtggc
     6601 ataactaccg gcactaagag caaagagaga aacagctaaa gcgatgcggt atttcattct
     6661 tgaaccttcc attgtgattc ggacctatta caaaggaacg attgtccaga caacgaagcg
     6721 gtagtgttaa ccaccgcctc attaatcata gacagttacg gtgaaatcac caaagtgagt
     6781 gatatgtttc ggataacagg aagttatccg aagcgatgag agttatcccg taaccgggtc
     6841 agccactgca tagcctgact gttattgtcc tgctgcgcaa tgatgtagcc atggggcaat
     6901 gttctgtcga ggaccgcttt tgcggcggcg ctctggtcgc tggagtcaaa tgtaattaac
     6961 agagtgtcgt ttttgggggt gatacttttg aaaggaatgc catgagcgtc aaggtgatgc
     7021 cagatcgaaa aaccgtctgg catcgttgtg ccttgatgaa cggcacgaat cgccagcgta
     7081 gactcttgct ggcgaaccgc ggaccaggcc agcaacagcg tgccgaccag aagtaaaaca
     7141 gtgccggacc aggctagctg gcgaagcgac atgcgaggta tttgcatccc ttacccccga
     7201 tttccatatt tctttttcca taacacgacc agagaacctg ctaggccaaa caccagcagc
     7261 accaccggga gcagcatcag gcatgacatc agctgttcct cgtactttaa aaataccggc
     7321 gttttgccga gcatgtaacc cagagttgtc aggatcaata cccacagcag accgctcatc
     7381 cagttgaaga actgaaagcg cgcgttattc agccctgata acccggcaat cgtcggcagc
     7441 agtgttctga caaatgcaat aaagcgacca attaacagcg ccgataaacc gtgtttatga
     7501 aaaagatggt gtgcgcgttg atgataatgc gcgggtaaat gagatagcca gttttgtacg
     7561 gtgcgggtat tgcccagcca tcgcccctga atatagctga cccagcaacc gaggctggcg
     7621 gcaacggtca gcagcagaat cgtttgcggg taacccatcg cgcctttcgc aatcaacacg
     7681 ccgaccaata ccagtaaact gtcgcccggt aaaaaggccg ccggaagcaa gccattttca
     7741 aggaacaaaa ttacaaacaa gacaaaatac aacatgccaa tcatcgatgg attggccagg
     7801 gtttcaaaat cctgcgccca cagggcttgc agcaattggg tcaaaagttc cattcattat
     7861 tcctggtgaa tcggttaacg ccacgctggt tttaaaagat gcactaccgc aatgttattg
     7921 ctgttttggt atgttctgcg ggtttgatac atcaacacag cctgaactat tccctgttaa
     7981 aacgtgagat aagaaagcat tcaaaagcat aaattcaaat tgaatctaat tgtaacaaag
     8041 gtcaacgttg ttcgttatca aaattacatg tccgtggagt ctgattttgc tgattgcagg
     8101 ggagggtggc gagaggattt acacaggctg acactttata cttttcgctt accgacgaga
     8161 caaacaggcg cattgcagcg cctgtcggac aggaatttca tttgctgccg ctggtggccg
     8221 tatcgagatg cactaccggg ttttcggcga acagatagcg gtcggcattg aactcgaaat
     8281 cgtcgctggt ttcgttaaat aacatgattt tggtgttttc cagatgctgc cacattgcca
     8341 gcttagcagc atgaggatct ttgcgaatta acgccttgag aatttgatcg tggtcatcac
     8401 accagttatc aacggtacgg gaatcaatgt gttcgtgtag ttttttccag tacgggttat
     8461 ggctacgctg ggtccacatt ttttcaacga tggccgccag ggcggagttc tgcgtcgcca
     8521 gggctacctg aatgtggaac tgtaaatccc actcggaatc acggaagcat tgttcgccgc
     8581 gcgcctgttc ctgaatggcc atcagtttca tgatgtcttg tttcgttacc tgagttgccg
     8641 cgaactcggc aatattactt tcgatgagct ggcgagcctg aagcaactca aacggaccgt
     8701 aattggcgaa ttccatatta ttgtcagccg cctgctgatg gcgcggctgg ttggaaacca
     8761 catgaatacc tgaaccttta cgcacctcca catagccttc aacttccagc atgatgatgg
     8821 cttcacgaac taccgttcgg ctgacgttct tttcatcggc aataaagcgt tcggcaggca
     8881 gtttatcacc caccagatag acgccctgtt caatgcgctc tttcaggtca gcagcaagtt
     8941 gttgatacaa acggcgtggt tcagtgattt ccatatgcgt ctcccggagt ggaatgcgga
     9001 cgaagttatc ataccacttt attcatgggt atgccattgg tcatatataa atttgatgat
     9061 tgaattaatg tattaaatta atattgtgaa tatttataaa ttcctaaaaa ataagtatcg
     9121 tcattgtgaa agctactaaa tttcagtaag ttaattccca gcctttaaat ttaatgttaa
     9181 ctaaaaatgc ttttttatat tgacttacaa taaatctaca tctctaatac accaactttt
     9241 ttgaaacgat attgtaagat caaaattata tggtggcgaa aattaatctc ttattacggc
     9301 ttacgccgct tttattcgtg ttcgctcctt ttttggcaca gtcaaatatg actgtttatc
     9361 ctatggctgt ttccattaat agtcagggtg aaggcaacgt tcgcgtaata tctaaaagta
     9421 atgaagttca gtacatcaag gcgacagtat tccgtatcga taatccatcg acgcctcagg
     9481 aaaatgaagt tgagattaaa tccggtgatg caaatcatct ggttgttatg ccacctaaat
     9541 tcgctttacc agccggtagt agcaaaaccg tacgttttgt tgcgatggaa ccagagcaaa
     9601 aagagaaaaa ttatcgcgtt aaatttgaag cggttcccag tattgatgac gttgccacag
     9661 ataaaaaaga tctctctatg cagttaacag ttaacttaat ttggggaatc gttgttagtg
     9721 ttccacctca gcaacctata gctaagttag aagtaaattc tgcccaaaaa ttaattaatg
     9781 cagagaatca acgcttaaag cttttaacga tcgcttattg taaaaataat agcaaagaaa
     9841 actgtaagat acagaccgta aataaaaaca tcttccctgg tcaggaaaga aatcttgaaa
     9901 gcatatctgg ctacgacaaa atcgttgtca aatataataa ctggatcacc aaagataatg
     9961 gcgagtttga actggcggtc cattaattat cattgtaata aacgaaggaa tagtaatgaa
    10021 aaaggtattt gcaaaatctc ttctggtcgc agcgatgttt tctgttgcag gctccgcatt
    10081 ggcagtacaa aaggatatta ctgtaacggc caacgttgat gccgctctgg atatgacaca
    10141 gaccgataac accgcgttgc cgaaagcagt agaaatgcaa tatctgccgg ggcagggtct
    10201 gcaatcttac cagttgatga ccaaaatctg gtctaacgac gttaccaaag atgtaaaaat
    10261 gcagttagtg tccccagcgc aactggttca aagcctggat gctagcaaga ttgttccgct
    10321 gacagtaacc tggggtggtg aagaaattaa agctgatgcg gcaaccactt ttaccgcaac
    10381 caaaatcttt gcttccgatg cgttaactaa cggttctctg gctaaaaacc tgatgtttgc
    10441 tcaaaccacc aaaggtgttc tggaaacagg catctaccgt ggtgtagtaa gcatttatct
    10501 gtctcaggat atttaatccg gataacagaa ttaacacgta acaaagggag gggtagccct
    10561 cccttatttt aactgtttta gtatttgtta tggatttcag gattgccatg gataaaaaat
    10621 tactggctct tttgatcctg gcgagtctca gcccggcaga ggcgacatta accaaaattc
    10681 ccgcagggtt tgaggttatt gctcagggac agcaggagta tatcgaggtt tatttttcag
    10741 ggaaaagtct cggtaaatat tatgcaatgg ttaatcttga taccgtaaca tttcttgatc
    10801 cagcaagtct atataacaag ctggaactgg atgtagacga tcagaaaatc gcgcatatag
    10861 tgaaagaaaa attatcgcag ccgctagctc gccacggtga attggcttgc ggttatgtac
    10921 gtactgactc agggtgtggt tttctgaata ccgatacgct ggaaataatc tataatgatg
    10981 aagaaagttc ggcaacgttg tttattaatc cgcaatggaa ttcagctttc gatgcgaagt
    11041 cattatattt aaatccagac aaaaatactg ttaacgcttt tatacatcag caagacatca
    11101 atgttctggc acaggatgat taccaatcgt tgtctattca gggaaacggt gcgctgggaa
    11161 taacagaaaa tagctatatt ggtgcacact ggaatttcaa cggttatgat gcagatgatg
    11221 tcagtgacag taatactgac gtcagcgatc tctattatcg ttatgatttt ttacgtcgtt
    11281 attatgtgca ggcgggtcgc atggacaacc gcacactatt taatgcacaa ggcgggaact
    11341 ttacctttaa ctttctgcca ctcggtgcaa tcgatgggat gcgtatcggg tcgacactga
    11401 gctatttaaa ctaggcgcaa agccagcaag gaaccccagt aatggttctg ctttcgcgca
    11461 attctcgtgt tgacgcttat cgtaatgagc aacttttggg atcgttttat ctcaatagtg
    11521 gttcgcaatt tattgatacc agttcctttc cgccaggcag ctacagcgtg gcattaaaag
    11581 tctacgaaaa taaccaactc acccgagctt gtgccgttta ccaaaaccgg cggtctgact
    11641 gacggaaatg cgcaatggtt cttacaggca ggtaaaacta catcacaggt ttctgatgat
    11701 gaaagttcag cttatcagct aggagtacgc ctgccattac atccgcaata tgagctctac
    11761 gcagggctgg cgaatgccga tgatgtgagt gctttcgagt taggtaataa ctggacggca
    11821 gatttaggcg gggtggggaa tcttgcaatc agcgccagcg tgttccgtaa cgatgacggc
    11881 ggcaaaggtg atatgcaaca ggccaactgg agtaatccgg gatggccgac gttgggcttt
    11941 tatcggacca actctgacgg cgatgcttgt acaaccgaca gcagagagag ctataacgcc
    12001 ttaagctgtt atgaaagtat ttccgcgaca gtttcactga attttgtcgg ctggaatatg
    12061 atgctgggtt atacccgcac acaaaataac actgatgata gtttgcgttg ggataaacag
    12121 cagagctttg aaaataacta tcttcgccag acaacagcgc aaagtatctc tgaaactgta
    12181 caacttagcg cttcccgcgc tattgtgatg cgtgactgga ttttgagtac gtcagtcggt
    12241 gttttccatc gtaatgacaa cggtggcgat aacgacgaca acggcttgta cttatcgttt
    12301 tcgttatctg acacgccaac gatggatagc aataacaaca gccattcaac caatgtgtct
    12361 acggattatc gttatagcga acaggatggc gatcaaacgt catggcagtt atcgcatacc
    12421 ttttataacg attcattcag ccataaagaa cttggcgtaa ccgtcggagg cctgaacacc
    12481 gataccataa acagcgcggt taacgggcgt tgggatgggc aatacggaaa tgtctacgct
    12541 accgtatctg atagttatga ccgtaagaat catgatcatc tttcggcctt tacgggcact
    12601 tatagctcta cgctggctgt cagtcgctat ggcgttaatt tgggagccag tggtacagac
    12661 gatttgctgg gtgcggtact ggtggatgtg aaaggcttct ctgaacagga tgaagagagt
    12721 caggatctgc aactcgaagc gcgggtggca ggcagccgaa ctttgcagct tggtcaaagt
    12781 gacagtgtgt tgttccctta tcctggattt cagtctggtt ttgttgaggt taacgacagt
    12841 agccagggca atcagcaggg gacaacaaac atcattaacg gtgcagggaa tcgtgaatta
    12901 atgttgttgc ctggcaagct gcgctatcgc gaagtgtctg ccagctttaa ctacaactat
    12961 atcggtcgtt tattactgcc ggcagcggtg aaaaaattcc cgattgtggg gctgaacagc
    13021 gccatgttac tggtagctga agatggcgga tttacacttg aaattaacgg cagcgaaaaa
    13081 gagctctatc tgctttccgg gcagcaattc cttaagtgtc cactgagtgt tgtaaagaaa
    13141 cgcgccagca ttcgttacag cggagatgtt acttgtagtg tggtgactta ttcacaatta
    13201 ccggagtcga ttcaggttca ggcacagttg aaacagccta aattacgtgg aaacgttcag
    13261 acggcgcaaa gggaggttgc accatgagaa atcgattgat tgcggcgata ttgggcttgt
    13321 ttggcacgct cactggcgtt caggcagctc ctgacgtgac cagtgaaatt acgtatgatt
    13381 tggcatctgg tagagcggat tattacttct ggaaggatga agcgtcggca ggaaataatg
    13441 gatatatgtg gtatgaatgt tcgtatcctg acctccaaca aacctgtaca gctaatggaa
    13501 atatatcgac agtacaaatc tatttaactg aacaacgcag tgggatgcgt tggccgataa
    13561 aactcaaagg atttaaaaca gccattgtaa gtagcgatga agcgccgcta ggatgcaagg
    13621 ggggcaaagg gcttcagacg aatcttaagg attctaatag atcttcatgt acagaagatg
    13681 gtcaacatta ttatatatac gatacaaagt ttcttacgct ctaccttgag cagacagaga
    13741 tgaagaattt gccgattggt ggcgtctgga aggggaaagt taaattacat tcgaacagcc
    13801 cggcccagga ctatttcgca aatattaccc tgaatacgct cgaccccaac catattgacg
    13861 tgttcttccc ggagttcgcc cacgccacgc caagggtgca gttagacttg catccaacag
    13921 gaagcgttaa cggcagcaac tacgcgcaag atctgaccat gttggacatg tgcctgtacg
    13981 atggttttaa cggtaatgcc atcagttatg aaatcatgct caaagatgaa gggcgaccag
    14041 ctgcagggcg cagagacggt tacttctcta tctatcgtca gggagggacc accaccgacg
    14101 agggagaacg cattgattac cgggtcaaaa tgtacaaccc ggaaaccggt gggcaaattg
    14161 atgtgcgcaa taatgaaaat atggtctgga acagcattaa cctgaaacgt gtgcgtccgg
    14221 tggtactgcc cggtattcgc tatgccgtga tgtgtgtgcc gacacccttg actctggcag
    14281 tcgataaatt cagcgtgatg gataaacagg ccgggtacta catgggcaaa ttgtcagtaa
    14341 tctttacgcc ttccttgcca accatcaatt aaccctgcaa accggacaaa cgcttttttc
    14401 tatataaacg acgaagccgc ctgataaggc ggcttcgtcg ttataactta cgttaaacgt
    14461 taatgttgcg gtgcgggatc gttatgtgtt tcctgcgcca cctcaatcgc cggtttgttc
    14521 tgcaatacgg tccagataac cagcgcaccc agcaggtcaa atactgccag aactgcgaac
    14581 agcgggctga agccgatggt gtcagccagt gcaccgacaa ccagcgcaaa cagcgtactt
    14641 gccagccatg cggacatccc ggttaaaccg ttcgccgtcg ccacttcgtt acgaccgaac
    14701 acgtcagaag agagcgtaat cagcgcgcca gacagtgcct ggtgggcaaa accaccgata
    14761 cacagcagca taattgcgac atacgggttg gtgaacagac cgatcatacc cgggccaatc
    14821 atcagcacag cacccagcgt tacgaccatc ttacgggaaa cgatcaggtt cacaccaaac
    14881 caacgctgga acagcggcgg caggtaacca ccgaggatac aaccgaggtc agcaaacagc
    14941 atcggcatcc aggcgaacat cgcgatctct ttcaggttaa agccgtaaac tttaaacatg
    15001 aacagcggga tccacgcgtt aaaagtaccc caggccggtt ctgccaggaa acgcggcagt
    15061 gcgatacccc agaactgacg gttacgcagg atctgaccaa cggacatttt cttcgccgtg
    15121 ctcacctggt gctgggcttc ctgaccatta ataatatagt cgcgttcttc atcggtcaga
    15181 tgcttctggt cgcgcggatg tttatagaaa atcagccatg ccatcgccca gataaagctc
    15241 aatgcaccgg agatgataaa tgccatctgc cagctgtgca tcacgattgc ccataccacc
    15301 agcggcggcg caatcatcgc accaatcgaa gaacctacgt taaagtagcc tactgcgatg
    15361 gaacgctctt tcgccgggaa ccattcggag ctggctttca gacccgccgg gatcatcgct
    15421 gcttccgcgg caccgaccgc accacgagca acagccaggc caccccagct acctgccagc
    15481 gcggttgcac cacagaacac ggcccacagc acagcaaaca ttgcataacc gattttcgta
    15541 cccagcacat ccagtacata acctgctacc ggttgcatga ccgtataagc agcagaatag
    15601 gctgcgatga tataggaata ctgttgggtg gagatgttta actcttccat cagagttggc
    15661 gcagctgccg ccacagtgtt acgcgtcagg taaccaagca cggtgccgag cgtcaccagt
    15721 gcgatcatat accaacgtaa ccctttaatt ttacgcatgt aaaacctcat cgttttgtta
    15781 tatccgcagg aagcgcgacc atcatcccgc cagaggctga agatgttagt tacgaccggg
    15841 taaccgaaaa aaaaccggca cgccccgaac gttgccatga aagtttcgtg ctaattcggc
    15901 ttccgtaccg gttaacccga ttcatctaac aaatgaatga atcggcaatt tgcattccgt
    15961 cgacttatga tttgcgacgg cagaaagata acttgtcata caaccttaaa aggtgagagc
    16021 catcacaaat gtgcgaatat ttgtagggac attacctgac gacagcgagg ccagtactgg
    16081 cgcggcctgc agcgagattt accactttga gagtaatttt tttaactacg tttattgatc
    16141 taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt cagcatcgca
    16201 ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt ttatgactga
    16261 agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg caaaagacca
    16321 gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag actatcgttt
    16381 taaaaacctg tatgacatct ggttgaaggg cgatcactac aaatggcgcg ctatgcgtac
    16441 caacggtgtg gctgagcgtc tgtgtactgg tgatgcgtct gaccgtgaaa aatttgacgc
    16501 ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga cgcacctcga
    16561 actgcgttgt ccgttcggta tcactggcaa attgctttct ccgtcaactg ccgatgaaat
    16621 ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg gcatcatgca
    16681 gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc tggagcatca
    16741 cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct ggcgtccgga
    16801 caaagccttc aatatcgaac aggcgacctt taacgactac atggcgaagc tgggcgaagt
    16861 ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca aacgtctgga
    16921 tcacttcgcc gctcacggct gtaaagtgtc tgaccatgcg ctggacgtgg tgatgtttgc
    16981 tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggctg gcgaaaccct
    17041 gagcgagcac gaagtggcac agttcaaaac tgcggtactg gtgttccttg gtgccgaata
    17101 tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata acaacctgcg
    17161 tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc gtccgatggc
    17221 ggaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctactgc cgaaaaccat
    17281 tctctactgc ctgaacccgc gcgataacga agtgctgggc accatgatcg gtaacttcca
    17341 gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta acgatcagaa
    17401 agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtctgctga gccgctttgt
    17461 cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat acttccgccg
    17521 cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg cggacatcaa
    17581 cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact acttcgccat
    17641 tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc gctggataac
    17701 gtcgcggtcg ctttagcaga tttggctgaa ggcacagaag tcagtgtcga taaccagact
    17761 gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga tatcgcaaaa
    17821 ggggccaacg tcatcaaata tggcctgccg attggttatg cattggcgga tattgcggcg
    17881 ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga tcagtatcgc
    17941 tatcaacctg attttcagga tctgcctgcg caagcggcag atcctgaagt gcagatctat
    18001 cgtcgcgcta acggcgatgt cggggtgcgt aacgagctgt ggatcctgcc aaccgtgggc
    18061 tgtgtcaacg gcatcgcgcg gcaaatccag aaccgtttcc tgaaagagac caacaacgcc
    18121 gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca actgggcgac
    18181 gatcacatca acacccgcac catgctgcaa aacatggtgc gccacccgaa cgcgggcgca
    18241 gtgctggtga ttggtctggg ctgtgaaaac aaccaggttg ccgcattccg tgaaacgttg
    18301 ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga tgatgagatc
    18361 gaagccggga tcgagcattt gcatcagctg tataacgtga tgcgcaacga taaacgcgag
    18421 ctaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga cggtctttct
    18481 ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc taacggcggt
    18541 actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct gatggaccat
    18601 tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt caaacagtac
    18661 tttattgccc atgatcagcc gatctacgaa aacccatcac cggggaacaa agcgggcggt
    18721 atcaccacgc tggaagacaa atcacttggc tgtacccaga aagcgggttc cagtgtcgtg
    18781 gttgacgtgc tgcgttacgg cgagcgtctg aaaacgccag ggctgaactt gttaagtgcg
    18841 ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca tatggtgctg
    18901 ttcagtactg gtcgcggcac gccatacggt ggatttgtgc cgacggtgaa aatcgccacc
    18961 aacagtgaac tggcggcgaa gaaaaaacac tggatcgact ttgacgcggg tcagctgatc
    19021 cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt tgagtttgcc
    19081 aacggtaagc aaacctgcaa cgagcgtaac gacttccgcg aactggcgat cttcaaaagc
    19141 ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc ataatgtgaa
    19201 gcgccgtttg cttttccata cctgctaagg atttatcatg accgcgtatt ggctggccca
    19261 gggcgtgggt gtcatcgcct ttctgattgg tatcacaaca tttttcaatc gtgacgaacg
    19321 tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt tttttcttct
    19381 gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat tgattacctt
    19441 acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg gaattggcct
    19501 cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg tcagtacctg
    19561 ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg atgtggtttt caacgtgttg
    19621 ctgggtgatt cacaacttct gggcggggtc gataggcggc acgatgattg agggtagttt
    19681 tctgcttatg aatggcctga atatcattcg tttctggcgg atgcagaaaa ggggaattga
    19741 tccgtttaaa gtagagaaaa cccctcccgc catagacgaa aggggttaaa caattaatta
    19801 cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc agtgaacagc
    19861 acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc gataataaag
    19921 ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt acaggccagt
    19981 gggatcagca gcaaagaacc ccccgccacg ccggatgcgc cacaggcaca cagagaagcc
    20041 actacgctca acagcagcgc tgtgggcaga tcgaccggaa tacccagcgt attaaccgca
    20101 gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt ggctcccagc
    20161 ggaatagaaa ctgaataggt atcgcgatcc agattcagct tttcacacag cgccatattc
    20221 accggaatgt tcgctgcaga gctgcgggtg aagaaggcat acacaccgct ttcgcgcagg
    20281 cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag caatgggtta
    20341 accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg cgcgtagccc
    20401 cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagcccaaa aataccgatc
    20461 ggtgcgaagc gaatgaccag tttcaccata aaggtaacgg cattcgacat atcattgacc
    20521 aggtttttgg tggtctcgtt accgtgacgc agtgcgaagc cgaggccgat cgcccacacc
    20581 agaatcccga tgtagttacc tttcagcagg gcgtcgatgg ggttggaaac catgctcatt
    20641 accagtccgc gcatcacttc gacaatgcct gacggcggcg aaatatcacc cgcgctactg
    20701 gacaagtgca gggtagaagg gaaggcaaag ctgaagacta ctgcggccag agctgcagag
    20761 aaggtgccca gcagatagag gaacaaaata ggacggatat tggttttctg cccgtgctgg
    20821 tggttagcaa tagatgccat taccagcatc aacaccagga tgggggcaac agctttcagt
    20881 gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt tgagatccat
    20941 gccagaagaa tccccagaac aaggccgacc aggatttgtt ttactaggct gccatgagcc
    21001 agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt tcattgtgtt
    21061 gtcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa agtgtttgca
    21121 aggtcgagta taaggaaagt tacagtttcg ggaagcgaaa aatgctggat tttaaagaga
    21181 ggtcatgttt tgcaacttaa tggttgcatt tatttaacat aaacagaggg aaggactggc
    21241 tactaaggct gattagataa catatgggca aaactatttg gaatatgaga acggttgcgc
    21301 tcctttaacg tgttatgcca tataaagtca ctggcgcagg gagcgcgcag ggggcggcca
    21361 atcgccgccg ccccctgcac ccccaggctc tggcgaacaa aatcgccgct gcgcggttcc
    21421 ctcagcctat cccttgcggc taccgggtcg ggcgcaaggt aacgtccctg taagatgcgc
    21481 cctcgccccg gcctccaggg aacgcttcgg cgattttgac gccaccaaca cccgtgcggt
    21541 ctttttgtaa agagctataa gccgattaca taattaatag ctcacctttg tctggaatga
    21601 gttatctcta ggaatggctt aatatacagc gaatcattct ccccgctgct tatcatgccg
    21661 ataattcacc caggcgttga taataaacgt catcaccaga atgccaaaca ccacgcccag
    21721 cgagacggcg attggaatat ggtagaagtc gacaatcagc atcttgatac cgataaacac
    21781 cagaatcacc gccaggccat atttgagcat cgagaaacgc tctgctacgc ccgccagcag
    21841 gaaatacatc gcacgcaggc cgaggattgc aaacaggttt gaggtcagca caatgaacgg
    21901 gtcagtggtc acggcgaaga tagccggaat gctatccacg gcaaaaatca cgtcgctcaa
    21961 ttccaccaga atcagtacca gcatcagcgg tgtggcgtac agcaatccat tcttacgcac
    22021 aaagaaatgc tcgttgtcga tggtgtcggt catgcgcaaa tgaccgcgta gccagcgcac
    22081 cagcggcttg tcgccaatgc ctgattcatc ttcatgggcg agcgccatct tcacgccggt
    22141 aaacagcagg aaggcaccga agatatacag gatccagtcg aactgcgaaa tcagccagct
    22201 gcctgtgaag atcatgatgg tacgcagaac aatcgccccg agcacgccat acaccagcac
    22261 gcgacgttgt aatgccgccg gaacggagaa atagctgaac aacatcagcc agacaaagac
    22321 gttatcgacc gccagcgatt tctcaatcag ataacccgtg agaaaggcca gggcctgtgg
    22381 atcggcaacg gcgcgacctt cggtttgcac cagataccac cagaaagcgg cgttaaacag
    22441 taacgacagc gtcacccaga ccagcgacca ggccgcagcc tgtttcatgg tcatggcatg
    22501 tgccccacga cgcccctgca acaacaggtc gatagccagc ataatggcga caacaacagc
    22561 gaatccgccc catagcaacg gcgtgccgac agtattcata gaagttcctt acacataaaa
    22621 aaacggccaa cgtcggaaga cgtcagccgc tgctttttat gcatagacct cgccttccgg
    22681 caaggtctca cttacaacaa aaaaggaata tgtctgcgta ttcctttcgg gttgcccggt
    22741 gaccggatgt ggtttttcac acatcgtaat gacgatcgac cggcaatgaa gttactcccc
    22801 tttgcaggta acaaagtatg acagaccatt cagtcagtca atggtctgtt acataccttt
    22861 tacactgttt tactttggca agtatagttt tacgctatct gccggaaaaa tcaccccagt
    22921 ctggcggcgg atctcggtca gcagtttggc tgtgatgcga ctgaccgcca gccctggatg
    22981 atccaccaga tgctcatcca ccagggtagc gaacagctct gcttcgtaga gcatggtatt
    23041 aatatgctgc ggctgggtga gatcctgcat ctggctgcca cgcggcacaa agcagacttt
    23101 ctggcattca gacagttttt caatcaccag cgctcccgct tcgccctgaa tctcgcttcc
    23161 caggacagaa tcactgacct ttgagtgctg caaggtgacg ctgaaatcac cgtaatccat
    23221 caccaccacg ccgtgggcgt caacgccgct tgccagcaaa ctcgccgttg cctgcacgct
    23281 tttcggctca ccaaataacg ccaccgccga cgccaggcag taaaagccga tatccataat
    23341 tgaaccgtta gagaatgccg gattaaaggt gttgggattc tcaccgtcca ggtaacgttg
    23401 ataccgcgag gaatactggc aatagttgaa aaagacttta cgcagtttgc ctgctttcgg
    23461 cagcgcctgg cgcaacaaat gaaagttcgg caggcaggcg gttttaaatg cctcaaacag
    23521 caccacctga ttttcgcgcg cacaggcaat ggcggcatcc acttccgcca gattcgacgc
    23581 cagcggtttt tcgcaaatca catgaatttt atggctaagg aaaagttgtg tctgggaaaa
    23641 gtgcagggaa ttcgggctgg caatatacac cgcgtcaatg gcatcgcttt ccgccatcgc
    23701 ttccagcgac gtaaacagat gctcgacaga aaaatcattg gcgaagtgct gagcctgctc
    23761 aaggctgcgg gaatatacgg cggttaactt gtatttaccg cgctcatggg cggcctcgac
    23821 gaactggcga gtgatccagt tcgtaccaat cacagcgaaa cgtatcataa cgcgtggata
    23881 ctccataaag ggattctgtc gtcaatgtag cacgcctttt tcatcttcga cgtgcgcaga
    23941 tacgcattat tctttcgccg gacagccatg ctcactaaac ggcccaatca tgaccgatgg
    24001 cccgagatat ttcggctggc gatggaggat ttttgcgtca atcaccgcgc tgacacgagc
    24061 gagtttctcc cgcagttgag tataaaaacc gtgcttcata ttgaggtctg gcgcgttaaa
    24121 cccgatagcg tcgatgccgt actgttttgc cagccagatg gcgcgttggt tatgaaattc
    24181 ttgcgagata atggtgatat ggttttcccc aaacaccttt ttggcacgca ctaccgaatc
    24241 cagcgttgag aatccggcat agtcgcaaaa gatcactttc gccggtacgc ctttagcgat
    24301 caaagcctgc tgcatacctg atgcttcatc gtaattttta tgtccgttat cgccactcac
    24361 cagtagccat ttcacttttc cggcgtggta caacgctgct gcggtatcaa tacgtcgggt
    24421 aaagtagcga ttacccggcc tcgcccccag caataaaccc acgttacgcg ccgggacggc
    24481 gttgacatcg ccccaggtca gctgcttgct ggcgttgacc ataacgcgat cggcgataaa
    24541 aatggtcgct cctgccacaa gtaacagtaa gcaggcaatc tttagagtcc ggcgagaaaa
    24601 acaaatacgg agaagaaggc gggcaaatgc gcgcagcatg gtcgttatcc ttaacgaaag
    24661 gggggaggtc gattttaggc gctaaagaaa gaaaagataa cgcctgacat cattttcaga
    24721 ttttgtcctg cccaagcgac aactgcgtta gccacagccg ggtgtcgaac tcaagctggt
    24781 ggtactgcgg ctccatatgg cagcacaatt gatagaacgc tttgttatgc tctttctcct
    24841 tcaggtgcgc cagctcgtgc accacgatca tgcgcaaaaa cggttccggc gcgttgcgaa
    24901 acacggtagc gacgcggatc tctgcttttg ctttcagctt gccgccctgc acacgagaaa
    24961 cagcggtatg taacccgagc gcgtttttta gcacatggat cttattgtca tacatcactt
    25021 tattgatcgg cggggcatta cgcagaaact gatttttcag atcctgagta tattgccaga
    25081 gagctttatc ggtggcgtag tcgtgtgttc ccggatagcg ttttgccagc acatcgccca
    25141 gacgctgttc gttaatcagt gttcgcacct gcgaaagcag ctgctcggga taaccctgaa
    25201 gataagtaag attgctcata accgcccacg aaaaagagaa aaagggtata ctcacgcacc
    25261 cttttcaggg gcaacgtcga aattttatca ttcaggaggg ccgatgagcc acttagacaa
    25321 cggtttccgt tcactgacac tacaacgttt tccggcgacg gatgacgtta acccgctaca
    25381 ggcgtgggaa gcggcagatg aatatttgct gcaacagttg gacgacacag aaatccccgg
    25441 cccggtgttg atcctgaatg atgcctttgg tgcgttaagc tgcgcgctgg cggaacataa
    25501 gccgtacagc attggcgact catacatcag tgaactggcg acgcgcgaga atttacgcct
    25561 aaacgggatt gatgagtcga gcgtgaagtt tctcgacagc accgccgact acccgcaaca
    25621 gccgggtgtg gtgctgatca aagtgccgaa aacactggca ttgctggaac agcaactgcg
    25681 tgcgctgcgc aaagtggtca cgtcggatac acgtattatt gccggtgcca aagcccgtga
    25741 cattcacact tccacgctgg aactgttcga aaaagtgctc ggcccgacca ccaccacgct
    25801 ggcatggaaa aaagcgcgcc tgattaattg cacgttcaat gaaccgccgc tggccgatgc
    25861 gccgcagacc gttagctgga agctggaagg tactgactgg actatccata accatgcgaa
    25921 tgtcttctcc cgcaccgggc ttgatatcgg cgcgcgcttc tttatgcaac atctgccaga
    25981 gaatctcgaa ggggagattg tcgatctcgg ttgcggtaat ggcgttattg gtctgacgct
    26041 gcttgataaa aacccgcagg cgaaagtggt gtttgtcgat gaatcgccga tggcggttgc
    26101 ttccagccgt ttgaacgttg aaaccaacat gccagaggcg ttggatcgca gcgagtttat
    26161 gatcaacaac gcgctctccg gcgtggagcc tttccgcttt aatgctgtgc tctgcaaccc
    26221 tccgtttcac caacaacatg cgctgaccga taacgtcgcc tgggagatgt tccaccacgc
    26281 ccgccgctgc ctgaaaatca acggcgagct gtatatcgtt gccaaccgcc atctggatta
    26341 cttccataaa ctgaagaaaa ttttcggcaa ctgcaccact attgcgacga ataataaatt
    26401 tgtggtgctg aaagtggtga agctgggacg tcgtcggtaa gattgggagt tacctgccgg
    26461 atgcggcgta aacgccttat ccggcctacg ttcagcacgt tacccccagg ccggataaga
    26521 cgcggcaagc gtcgcatccg gcaatgtctg ctcagttctg gaatcgcgct tccataaaac
    26581 aaaaaatccc tgtcaccatc ctctacattc tcttgtttag cgtttttcta cgtttattct
    26641 tccgtcacac agataaattc atccgttgcc aatctgtcaa cggatgttat catattgcca
    26701 atttgtcaac ggacgtgatg aagaatgcac ctgataactc aaaaagcatt gaaagatgct
    26761 gcggaaaaat acccgcaaca taaaacggag ttggtggctc tggggaacac gattgctaag
    26821 ggatatttca aaaaacctga gtcattaaaa gcagtattcc catctctgga taacttcaaa
    26881 tatctggata agcattatgt tttcaatgtt gggggcaatg aattacgtgt tgtagcaatg
    26941 gtcttttttg aatcgcaaaa gtgctacata cgtgaagtta tgacgcataa agaatacgat
    27001 ttctttaccg ctgttcatcg tactaaggag aaaaaatgat tgcgattgcc gacatcttgc
    27061 aagcaggaga aaagctaact gctgtggcac cttttctggc gggtattcag aacgaggaac
    27121 aatacaccca ggcgctggaa ctggtagatc atctgctgct caacgatcct gaaaacccct
    27181 tgctggatct ggtgtgtgcc aaaataaccg cgtgggaaga atcagcgccc gaatttgcgg
    27241 aatttaatgc catggctcaa gccatgcctg gcggtatagc cgtgattcgt acccttatgg
    27301 atcaatatgg tttaaccctt tccgatctgc cggaaattgg cagtaaatct atggtgtcac
    27361 gcgttttgag cgggaagagg aaattaacgc tggaacacgc taaaaaattg gcaacgcgat
    27421 tcggcatttc tcccgccttg tttattgatt aacgcgacgt gccggatgcg ttgcatccgg
    27481 cgcttcaaat aacgatttaa atctccagcg ccagccgtgt tccctgggca attgcccgtc
    27541 gtgcgtccag ctccatagcc acatcgcagc cgccaattaa atgcacagtt ttcccgctat
    27601 caatcagcgg ttgcgccagc gcgcggtttg gctcttgccc tgcgcagatc accacattgt
    27661 ccactgctaa tacctgcgtt tcgccgttga tcaccacatg cagcccgtca tcgtcaatct
    27721 tctgataact tacgcctggg atcattttca caccccgcga gagcagggtg gtgcgatgga
    27781 tccagccggt ggttttgcct aacccctgtc ccggtttgct ggctttgcgc tggagcatca
    27841 caatttgccg tgggctacgc gggatctgca ttccctgcgg gcttaagcca ccagcctgtt
    27901 gtaggctgct gtcgatcccc cactcattac agaacctggc gatattctgg ctggtggatt
    27961 cgcccggctg acttaaatac atcgccgtat caaaaccaat cccgccacaa ccgatgatgg
    28021 caaccttgtt gcctaccggc gctttgtcgc gcagtacatc gagataactc aataccttcg
    28081 gatgatcgat cccgtcgata ggcggaacgc gcggcacgat cccactggcg aggatcgttt
    28141 catcgaacgc ctgtaactga tccgccgtca cggtgtgatt gagtttcagc gtcacgcccg
    28201 tcacttcgat catccggcgg tagtagcgca gcgtttcgta aaactcctct ttgccgggga
    28261 tctgtttggc gatattaaac tgcccgccga tctcgctgtg cgcatcaaac aatgttacct
    28321 gatgcccacg cgccgccgcg ttaatggcaa acgccagccc agcaggtcca gcgccgacca
    28381 ccgccagatt ttttttctgc acggcgggaa ggattggcat tttggtttcg tggcaggcgc
    28441 gaggattcac caggcacgag gtgactttgc caacgaagat ctgatcgagg caggcctggt
    28501 tgcagccaat acaagtgttg atctcatcgg ctcgccctga ttgcgctttt gacagcagtt
    28561 ccgcatccgc gagaaacggt cgcgccatcg atactatatc ggcatcgccg cgcgagagaa
    28621 tatcgtcggc aacctgcgga tcgttaatcc ggttggtggt taccagcggc agcgagacgt
    28681 ggcctttcag tttgcgcgtg acccagctaa atgcgccgcg cggcacgggc gtggcgatgg
    28741 tcggaatccg cgcttcgtgc cagcctattc cggtgttgat aatggtcgcg cccgccgctt
    28801 caatggcctg cgccagttct accgtttcgg caaaagtgcc gccgccttcg accaggtcga
    28861 gcatcgacaa ccggtaaata ataatgaagt cgttgccgac gcgttcgcgc accgtacgca
    28921 ctacttctac ggcaaatcgc atccggttgc ggtaatcgcc gccccactgg tcactacgct
    28981 gattggtgcg cagcgtcaga aattcgttga tcaaataccc ttcagagccc atcacttcga
    29041 cgccatcgta tccggcctcc cgcgccagtt gcgcgcagcg ggcgaaatcg tcgatcagtt
    29101 gcaggatctc ttcatgggtg agttcgtgag gaacgaaacg gttgatgggg gcctgcaatg
    29161 cggacggtgc gaccagatgc ggttggtagc tgtagcgccc ggtatgcaaa atttgcaggg
    29221 tgattttgcc gccttcctga tgtacggctt cggtaatggt gcgatggtgt gggatctggc
    29281 tggcgtcgtt gagcattgcg ccaccttcca tgccaacgcc tgttaaatct ggtgcaatgc
    29341 cgccgctgac aatcagcgcc acgccgtgac gggcgcgttc ggcataaaac gcagccagcc
    29401 gttcagcacc gtccgggtat tcctccagcc cggtgtgcat tgaacccatc aacacgcggt
    29461 tttttaacgt ggtaaaacct aaatccagcg gggcgaacag cgacgggtag ctcataaaat
    29521 tgtccagtat gtaaaataat tgttatgtgg tcggatgagt tctaatttag ccgtcgatgg
    29581 agaaaaggga aaaggggagt gcggggtttg tgatgggatt caaaaagaat acgcccggtg
    29641 gtaacgccgg atgcgacgct tgccgtgttt tatccggctt gggggattgc acatgtaggg
    29701 cggataaggc gttcacgccg catccgccag ccgttgcaaa ttactgctta cggaaaaaat
    29761 cgttatacaa catatacaaa tgagcggcac tccaggagaa atttggtgcg ccttgctgcg
    29821 cgcctgtcag cgggttgtaa ttctcctgaa ttgggccatc ggcggttaat cctttggcgt
    29881 gcctgaagaa cgtatccgcc agtttcaggg catcatcacg ataaccgtaa cgctccatcc
    29941 ctttcagacc aaaccagaac tgatccaccc atacgcgccc gcgccagtag atatcagcgc
    30001 caaaagccgg attggttaac gccgccgttc ccagcggaac aaaggtgttg aactctttag
    30061 gatcgagcat caccttcacc accgcgtcgg cattggcctg cgttgccgca ccgttaaaca
    30121 gcggcgacca gccttccggc cctttaccgc gctcaacaat cggtttgccc gcgcagccgt
    30181 tcgccagtgg tttatcttca atacgtacgt catagtagaa ctgggtagtc gggtcgaaca
    30241 tacaggtgtt gatgtagtcc gcgagctgct gtgccaactg gcgatagcgt ttggcctctt
    30301 ctggcttacc gaggatggtt gccatctccg ccagataatg gttatcgctg tacatatagc
    30361 tggcctgatc caccgactcc tgcaatagcg agtagcccag caacgttccg tcctgactgc
    30421 ggttttcggc gaatttcacc gtccagtcgc tacgtttgcc gccattagcg acatatttat
    30481 ccaactgttc tttgtcgata aaaccaaaga cggcggcatc atcacgcccc gattcccacg
    30541 acgcagcgac ctgtgccgga atttccagac tgtcgtactg gcctttctcc accacgcggg
    30601 cgtagttgtt cagcccagac tgcgtctctt ctttatcgcc ttttttcact gtaaacagca
    30661 tctcgccgct ctcggtgttg tgggctttgt cgcgggtcgc gccatattcc ggcaccccgt
    30721 tgccgttatg atcgcggtta cgtaaccacc agtcgtgata ggccaccagt ttcgggtaca
    30781 tctctgccaa ccaggcttta tcctaggtga cgttgtatac ttccatcacc gaccaggcgg
    30841 caaggctggg tttggtatta cgttcgttcc agttgccgcc atcgccacca cgctcggggc
    30901 taaggttcca cgctatcagg tcggggacaa agcccacatc ctgcggacgc acgctgtcgc
    30961 caggctggat ctgccaggag aagaccgcgc ggatattctc tttggcgata tccggattga
    31021 aatgcaccat cgcaaacgcc tgcttccagg tatcccacgg ccaggtctga ttgccggaga
    31081 accagcgtcc ggtcaccgac ggcgtgacgg tgttataatt caccgcaccg cccggtgagc
    31141 gccagttacc gttgagcgtt tcgatcgctt tcaccgcgac gcgcgtctgt tccggcgtcg
    31201 catccggatt ggttaaccct ttcttcaggt attcttccca gcgttgctgc gaggcggtga
    31261 gataaaacgc cggacgcgcc agaatatcgc ggatctgcat ttgctctttg ctaacttcct
    31321 gagcggtcag cagatgggaa taggtggtgt agagcgtggt cgaaccgttg atatgcgcct
    31381 tactggtaaa gcgattaccg ttgatttcag tctgcaccgg cagggattta tgcacctgat
    31441 attccgattc gccggaggtc agcagatccc aggtggcgcg cactttgcca aaggtgactt
    31501 tcaggccatc acgggtggcg ctgattttgc gctgatagtc agggtattcg ccagcaatgg
    31561 ttttatcgga aagcggtttc ccttctttcg cttccagttt ttccagcagt tcgccatccc
    31621 acaccagatc cagtggttta ttgctgatga ttttagtttc cagtagtgac gtgcgcggcg
    31681 tggcgaagcg cagagtcatt tcgacctgca catcttttgc tgtcagtttt tgcaccagcg
    31741 cgccgggaat actgtatgcc tccagcgtga agttgacttt cttgccgtct tgccagacag
    31801 tcaggcggtc gaaattgctg gccataaagt tgatgtattc ttccgtcagc agcgcaacgc
    31861 ccggaaagcc gcccatggtg ttcgggccgt ctggcaacag atgaccatgc caggctccga
    31921 gatcgaaaaa cggattaaaa cgctggtgat cgtcgtaatc gtaatccttc atgtactgcg
    31981 gcgcaccagt acggttaatc acgtttttat aattgtcggc gttagcggca tgagcggaaa
    32041 aacttatcag cagagcgcag gttactggcg ttaaaatagt tttgattttc atgaaattac
    32101 agtccttatt accagtagaa atattgcatc aggaagattt ggtcgctggg atcgttagag
    32161 gcatttttca tgtagaagcg gctatcaaaa gccgtggcga aacgtccgcc gccatattcg
    32221 taccagatac caaactgcat gaaagaggtg gtttcatcgt cagcgtggtc agggcgatgt
    32281 ttggcgtagg tgtaagcggt ggagagataa acgcccttta acgcttcata catggcgctc
    32341 accatcatcc cgctttcact tcccggtacg tccagcccgt cggcacgtcc ggtatggtgc
    32401 caggcgcgag cggcaaagtt aggggtaaac aggcccgtga gatagagttg tcctgtgccg
    32461 tcggtaattt ccagaccatt catccaggtg atgccttcgg caaggtcata ctggatgtaa
    32521 ccgttcatcc attgccggat aggtgtattt gtcgtcgtag cgatcgtact tgccaaaatg
    32581 tagccaggct ttgctttcgt catgatgtcc ggcaggtgtg gcggtgacgc tataacgaaa
    32641 gttgccgctt aagttctgta ccttcagcgc ggtcataatg tcgcgggtat tgggaatgac
    32701 gtagcccaaa tcgggtgtga agtcgcccca ccattgcagg tcatccagcg aggaatcctg
    32761 acgcagactg agcatgattt cggtgccatc attggtacga taaccgccat agaaacggtt
    32821 aatcccacct tcgaaaccac cccagctatg gtcaggcacc caggcattac cgtcgtggtc
    32881 ggcctgcacg gtccagcctt cgccgtaaat caggccgaac caggagccgt acttcatctc
    32941 caggccacct tcaatatagg tgccgtcgct gtatttatgt ttattctcac cctctaaatc
    33001 catatatcca ccaagcccta attcgccgta aaaacgaaat gaaggagaag atgtcgaggt
    33061 aattgcgggt gtttttgtgt cgtcatgttc tgccggacgt gcttcttctc ctgccgcaga
    33121 aaaggcgtag caaaacggca gagcaagtaa tgctctgcat ggtgcagtga ttaatttcat
    33181 tgtgttaatc catgagctaa agaaaaagta cagcctttta tcgttgtaat tataaaaata
    33241 taaaggcaga aactattttc cctctcctca tccaggaaga gggaaaatgc ctgctcattt
    33301 tttattagtc tgcttaatca acattgctgg ctggcgtcgc ttcaatatgg cgctcttcag
    33361 ccgttaatcc ctttatatat ttactgtatt tccaccacgc aaagccgagg aagataacaa
    33421 taccgccgac gttataaaaa atgatggtca gaatattcgc gccagtcggg aatgtcgaag
    33481 cgacaaatcc tacggcaaat atcgcaatca gcattgaaac aacgataata ccggtgcggc
    33541 gggagcccat acggaaatcg cgtggcaggt gatctaattt ggcgcgtaaa ttcaggtaag
    33601 ccagcatgat gaataacggc ggaagcatgg acgctgcggc ggtcatatta ataatggtat
    33661 tcatcagatc ctgcacggta ttggaaccga gcatcgggat aatcatcagc gggatgacga
    33721 tcagaaactg gatccatgct gcgcgcgccg gaacgccgtt ttcgttaagt tcgacggttt
    33781 tcttaccaaa gatgccttcc gggatttcgg agaagaaaat tttcaccggc gttgcggtcc
    33841 acatcagcag ggaaccgaac attgcggtaa aggacaccag accgacaaag cgattcatca
    33901 acgcttccgg cagaccaaaa tacgccgcca tgccgtggaa tacctgcacc gatccgccgg
    33961 taaatttcaa ctctttgctg ctgacgaaga cgttaatcag caccgaggag acggaataca
    34021 gtacgccgat aaaaatcccg gcgaggatga tcactttaac gaacgatttc gaaccgcctt
    34081 tgacgtcgtt aacgtacacc gcgacggact ccgcgccacc tgcggccata aagatccagg
    34141 tggtaacgcc gaggaacgcc cagttgaagt tcgggatcat cgcgtcaacg gtgatcggat
    34201 cggcaggctg tacgccgcca accagtgccg tacctgccag taaaatgtag gagagcgtta
    34261 acagcagcat cagcgttgaa gtgacggagg taattggccc cagcattttc gccccgttgg
    34321 tggaaaccca ggtggagaag gcgaacagca ccatactgat aacggtggtg gcaaccggcg
    34381 tcataatata ttcgtagccg aggaaggcat acgaagcata agcaataacg cgcggcaaca
    34441 gcgaggtgaa aaagaacaga tttacgaacc agtaagtgta ggcagtaata aatgcccaac
    34501 gtccgcccag cgaacttttt acccacgcgt agacaccggc ttctgagttt ttatttaacg
    34561 aaacaaattc tgcgatgatc agacagaagg gaataaaata aaaaatcgtc gcgaggaaaa
    34621 acatcggtgc cgaggccagt ccaagctcaa tattattatt gataacgttg ttaaagctgt
    34681 aaacggcggc aaaagtcagc gagagcaagc cgaatttgcc gattgtatta cgtttggtat
    34741 cagacataac acatctccga aaatgtgtag ggtagcggtg gcggcaaact ccttttgccg
    34801 ccgtagttgt tatttgttat ggaaataacc atcttcgatg gtgactttga gaaccacttt
    34861 tttgaccgcg ttattgcaga taaaccgata cgcttcatgg atatcgcaaa taacgatttg
    34921 cccttcgtgg acctctacgg tttctccgca gccttttaaa tattcacggt cagtttcatc
    34981 acgataatat tccactacct gtaatgtctc tttcggcgca tattcaattt tttgctgccc
    35041 ttgcaggtag taatgcactt caaaatagcg tcgatgcccg gtaaatagcg catcggtcgc
    35101 ggagtcattt tccacacggt aggtcaatga atcaccgatg gagtgggcga cgccaggctg
    35161 aatgttgtcg atattttcaa tcgcttcaac gcagcgttgc cacttcttgc cagaggcgta
    35221 aatctggcgg aactgttcta agttatcgat gatcctcatt gcttattctc gctgtgcaaa
    35281 ttcgtggaaa agaaccctgc gccgaactca tacgacgcca ggctttgcgc ggtagcttct
    35341 ccgccagaaa ccggcagcaa cgtaaagccg tagctgaagt cacggaacca gacgcgccag
    35401 gagtccagca cctcgctgcc ccaggagttg gagccgaggc caagtagctg gtggtcgagg
    35461 ttcagggtga tgtcatcact gcgctgcagc tcgttacagt gctgggcagc gtgaatgttt
    35521 tcctgggtat agtgccaggc gctgaagtta attgggcgct gcggaaccac cagcagaccg
    35581 ttgccgtggc ggttagtcag tgccgtccag cggacatgct gacggttgcc gttgttctgc
    35641 gggaagggat agttctcgaa catggcatcg acggtgctgc gccagatatc gatgatgtta
    35701 gcctgctggc tgtcggcgta gttttcgccc ggtccacgac cgtaatacgc cacctgatcg
    35761 tattcgccgt taatccccat ggtgaaaccg atgcacggaa tgatgtgcgg atagtcgccg
    35821 taacgctcgc cggaaagcgc cacgttaacc tggccatcgg cagcgatgcg ccagatgtag
    35881 gtacagcgca tcccgaagtc aaacaccggc ggtgcaataa ctgtgcggct gatgatcaac
    35941 acttcaccat cgctctgttc tacggcaaag tcgcgcagat gttcctgcat gatctgcaaa
    36001 tgattcggct gccacaaccc ttcgtactcc tgcttgtggt tgtcgatcat cggcttgaag
    36061 aagttgatct ttggctcgcg ggtcagcagc gactcgccat ttacctgcca ggatgtcggt
    36121 ttgccactca tttttgagaa ggtgatcgcg aagttgtagc cgcgaacggt gcagctcaaa
    36181 cgatcgtctt ccagcgtcag cggacgcgca ttatttggtg cgaaaggcac tggctgcgcg
    36241 gtgttttcct tcagcgggaa ctgataagtg gcgatagaat gcccggcttc gctgtagcgg
    36301 gtgtgggaat ctttggtcac cgtaatgttg aggaacgctt cgcgggcgtc cagctgcggc
    36361 agcgtgatct gcaagggggc ttcgctgttc ggcgcaacgt cgcgcagttt aatctgctgc
    36421 gtcgcgagcg tttcaccttc ggcgcgcacc tctgcgtgca gggtgtagtc atcaagcgtg
    36481 gtaaaccaca gtttattttc gactttcaac tcgccgcgag tcagatcccg cgcgtggatt
    36541 tttaccggcg cgataacctg tttgtactct ttcaggcccg atcccggcgt ctgatcggaa
    36601 tagatcaaac catcaagaca gaagttatag ttgttgggat agtcgccgta gtcgccgccg
    36661 aatttatacc agacattgcc gtggtcgtcc tgtgcctgga tcccgtggtc gcaccactcc
    36721 cagacataat gaccctgaat gcaatcgtgc ttatagaaga cgttctggta ctccgtcagc
    36781 ccgcccggtc cgttccccat cgcatgagca tattcacaga tgatgcgcgg cttcggatgc
    36841 gggtattcac caaactcatt catcagcggc acgcgggtgt acatggtgga aataatatcg
    36901 accacttcag catcgcgatc ttcttcgtaa tgcaccagtc gcgtgtcatc cagcgctttc
    36961 gccgcatggt acatcgcgcg gatgttacag ccatagccgg attcattgcc cagcgaccag
    37021 atgatgatcg acggatggtt tttctgtgcg tggatatggc gaacaatgcg ctcgacgtag
    37081 actttttccc actgcggatc gtcggtaata cggctgatat cgccgacatt agcaaagccg
    37141 tgcgattcga cgtcggtttc cgccatcaca aacaggccgt agatatcaca cagttcgtaa
    37201 aaacgcggat cgttcgggta gtgagcggta cgcacggagt tgatattgtg ctgcttcatc
    37261 aactggagat ctttctcgac gcgatccatt ccaacggcgc ggcctttgcg atgatcgttg
    37321 tcgtgacggt tgacgccgtg cagcatcaca taacggttat tgatccagaa cagaccgtcg
    37381 cgcactttga tatcacggaa gccaacgcgt tgtggcacca cttccagaac gttgccgttg
    37441 gcgtctttca gcgtcatgac cagatggtaa agataagggg attctgctga ccattgctgc
    37501 ggctgttcga cagtaaaagc aaagctggcg ctggtcagtt tttcaattgc caaatgatca
    37561 atggcgctgc tgtgcaccac gcgttcgcca tcaaacaggg tatattccag cgtcgtgacg
    37621 acaggggagg cggcgagatt ttccagcacc acttcgcagg aaagcgtggc atcgcaatag
    37681 gcttcgtcaa agtcggtacg cacagtgaaa tcgttaatat gcgttaggtg ttttccgacc
    37741 agataaacat cgcggaagat ccccgccgac caccacatat cctggtcttc cacgtaggta
    37801 gagtccgccc actgcatcac gcgcacacac aacaggttgt cgccggtttt aaccatcgcg
    37861 ctgatgtcaa actctgcggt caggcgactg cccttgctga aacccacata ctgaccgtta
    37921 acgtagactt caaaataggt ttcgacgccg tcaaatttaa tcagcgtctg tttaccctgc
    37981 cagccgtcgc tgagggtgaa aatacgttga taggcaccgg ttgggttatc gctggggaca
    38041 aacggcacat cgatggggaa cggaaaacct tcgtcggtat attgcagttt gccgtgacct
    38101 tccatttgcc acatggcggg gacggtaata tgcccccagt cagccattaa ctcagaggtg
    38161 aaggcttctg gtacttgcag cggatggtca aaaaagtgga aattccactg accgcttaag
    38221 ggcagaaaca ggctgctggt ttcgcgggca aaggtacgcg cttgcgcaac agaatcatat
    38281 gaaaaaaagt acgcacgcgg cgcaagtcgg ttttcgtggg tgagctgaat gttttcccag
    38341 cgattcatag ggcctcccgg tagagagaaa tcggaaccat tcatggtgat ggtagtttgt
    38401 gtaattgctg gggagtttag taaaattttt actaaaaagt agcaggtaaa tgcatttact
    38461 ttaactggat cacgaaaata aacggcagca ctgtccggca aaaatgattg ccggacagtg
    38521 tgtaagggga tttaacgggt cgtgccgcgc agttttaatt tgctgggaac gaagactagc
    38581 agcggcagcg cgcgaccatc gcgggctttt tcatacacca ggttaacgcc ctgacttccc
    38641 atcatctcgg aatggatgcg cacggtggag agcggcggaa aggtaaatcg cgcggtgggg
    38701 atatcgttaa cgctgataag cgaaatatcc tgtgggatgt tcaggccacg ttcatgaatt
    38761 gcccgcagta cgccgatagc aatggaatcg gaagcaacaa acagtgcctt cggatagtct
    38821 tcccgcgcca gcatttgttt tgccagttca taacccgacg aactggaaaa accgccgcgc
    38881 cagatatcct cttcgcgtac cacttgtttc agtcggccat attccgcaaa ggcgacctca
    38941 cgaatatccg ccttgccagg ctcatcttca ccgccaataa aaccaatacg attaacgccc
    39001 tggttgatat agaagtcgat gatttcttta ctgatgcgtg ccagatcgat atccaccgca
    39061 tcgtaaccgc tgccgggttc gtgaaagtcg ataaaacaga tattgtcggt caacgcgctg
    39121 gcagcggcgc gcagggcggg cgtgggtttg ccgacaatta aaataccggt gacgttttta
    39181 atgtctggta atccgttgtg ttcataacag ttggtcagtt cgatcgccag cttttcgcac
    39241 tgagtttcaa tgccgtggcg gatcgccaga tagtaaggat cgttgatctc cagctcctgc
    39301 tggtagctgt agatagccag aatatggtgt tggttgactg cgcctgtctg tagtttacgg
    39361 gcgctactgg tcttgtactc cagcttttcg gcgatctcga gaatgcgatg tttcgtctct
    39421 tctttcacat tcaatgtcgg atcgtcattt aagaccctgg atactgtcgc cagggatacg
    39481 ccagcttcga ttgcgatgtc ttttaatgtt gccatgttta ccttttttac tttccctgat
    39541 gtgcgccccc attgtagagg aagtgcttat cgggtcacct gttttcagta aataaagcgc
    39601 gtttctttga cgctgtaacg gtaacacagc ggcttaggaa tgtgatcacg cctgcattct
    39661 ccatttttag cctacaggat tgagaaaaac ttttgctata gtttagtaaa attttacccg
    39721 aagggaggaa gcaatggaaa ccgtggctta cgctgatttt gcacgtctgg aaatgcgcgt
    39781 cggaaagatt gtggaagtga aacgccatga aaacgccgac aagctgtaca tcgtacaggt
    39841 tgatgtgggg aaaaaacact gcaaaccgtg accagcctgg tgccgtacta cagcgaagag
    39901 gagctgatgg ggaaaacggt ggtggtctta tgcaatctgc aaaaagcgaa aatgcgtggt
    39961 gaaacgtcgg aatgcatgct gttgtgtgcg gaaaccgatg acggcagtga aagcgtgttg
    40021 ttaacgccgg agcggatgat gcctgcgggc gtacgcgtcg tataaaaaga tcggatggcg
    40081 acgtcgtatc gccatccgat ttgatattac gcttcttcga cacttactcg catggccgcc
    40141 agcgccttac gcgccgcttt gatcaccagt tcacactgtt caatggtcag tgtcagtggc
    40201 ggttcaatgc ggatcgtttt ggcgttattg agcgttccgg ccaccagtac gcgctggcgg
    40261 aacatctcgc tggcaaagtt atagccgatt tcgttatcaa caaactcaat cgccatcaac
    40321 atccctttac cacgcgcttc ctgtaccaga tcgggatatt cccgcgccag ttgacggaaa
    40381 ccatccagca acatatcgcc tttttgctca gcctgagccg gtaagttctg ctccagcaac
    40441 acattgatgg tcgccagcgc cgccgcacag gccagcgggt tgccgccaaa ggtggtggta
    40501 tgcaggaatg ggttgtcgaa cagaactgaa aacacctctt cagtggcgat ggtcgcgcca
    40561 atcggcatca cgccgccgcc gagggctttt gccagacaca gaatgtccgg ctgaacgttc
    40621 tcatgttcgc aggcaaacat cttgcccgtg cgccccatgc ccgtttgtac ttcatcgagg
    40681 atcatcagtg cgccgaactc atcgcacagc ttacgcacgg cggtgagata gcccggcggc
    40741 ggcagaatta cgccaccttc gccctgaatc ggttcgagga tcaccgcagc cacatcatca
    40801 ccggtttttt tgcactcatt aagagccgtg cgcatggctt cgatattgcc aaacggcaca
    40861 tgacggaacc ctggcagcag tggcataaac ggtttgcgga aggtcgattt cgccgtggcc
    40921 gacagcgcgc caagtgattt accgtggaac gcgccgctgg tggcaataaa agtaaatttg
    40981 ccgcgcggcg actgataagc cttcgccagc ttcagcgccg cttcaacgga ctcggtgccg
    41041 ctattacaga agaagctgta tttcagttta ccgggcgtta gcgcagcaag ggttttcgcc
    41101 agcatagccc gtaacggatc gagtagctcc tggctgtgca gtggttgttt cgcaagttga
    41161 ttctgtacgg cggaaaccac aactggatta cggtgcccca cgttgaaaat tccaaaacct
    41221 cccaggcagt cgataaactc ctgtccctgg gtgtcgacaa gcgtatttaa acctcccgct
    41281 tgccactcta cggctccgta atccccgccg gcggtaacag atttgcgata ctctaaaaac
    41341 cccggattga catgctcttt gaagtattca atcacctctc ggttaagtgc tttcatctcc
    41401 tcatgatcca gcgttcgctt ctcaatgaga ttcagggcgt gggcgctgca cgctaaagcc
    41461 gatgcgctcg aaggtaacct gttcaaaata tgctccggag gctcgcgtat cacatgatac
    41521 cgatttaagt attgcaggga ttgcgccact ccggctgcga tcgcgaaaat cgggataaac
    41581 acaaatgcgg ttatcatttg tgcaatattt aatgctatcc gttaacattt gttgttattt
    41641 tgcacggttt ttgtcgggta tggatttgat gattgctgcg caaatgcacg ttttcagggc
    41701 aataattgcc tgatttttgg gcaagataat taacgttttc gtatagtaac agagtgaaag
    41761 tattttaaat caaagaatta gatttgtgct ttaatcgcaa attgcgatct aaatcaaatt
    41821 aatcggttaa agataaccgc agcggggccg acataaactc tgacaagaag ttaacaacca
    41881 tataacctgc acaggacgcg aacatgtctt ctcatccgta tgtcacccag caaaataccc
    41941 cgctggcgga cgataccact ctgatgtcca ctaccgatct gcaaagctat atcactcatg
    42001 ctaatgacac ttttgtgcag gtgagcggct ttaccttgca agagttacaa gggcagccgc
    42061 acaacatggt gcgtcacccg gatatgccaa aagcggcgtt tgcggatatg tggttcaccc
    42121 tgaaaaaagg ggagccctgg agcggcatcg tgaaaaatcg ccgcaaaaat ggcgaccatt
    42181 attgggtgcg ggccaatgcg gtaccgatgg tgcgcgaggg aaaaatcagt ggctatatgt
    42241 cgattcgtac ccgggcgacg gatgaagaga ttgcggcggt ggagccgctg tacaaagcgt
    42301 tgaactccgg acgtaccagt aagcgtattc ataaaggcct ggtggtgcgt aaaggctggc
    42361 tgggtaaact gccttcatta ccgcttcgct ggcggacgcg tggagtgatg accctgatgt
    42421 ttatcttgct ggcggccata ctttggtttg ttgctgcccc ggtggtgacg tatttcctct
    42481 gtgtgttagt ggtattgttg gcaagcgctt gttttgaatg gcagattgtg cgcccgatag
    42541 aaaatgtcgc ccgtcaggca ctgaaggtgg cgaccggaga gcgtaatagt gttgagcacc
    42601 tgaatcgcag cgatgagctg gggctgacat tacgcgcggt agggcagctt ggcctgatgt
    42661 gccgttggct aattaacgat gtctcaagcc aggtgtccag tgtcagaaat ggcagtgaga
    42721 cgctggcgaa aggcaccgat gaactgaacg aacataccca gcagacagtt gataacgttc
    42781 agcaaacggt ggcgatcatg aaccaaatgg cggcgtcggt gaaacagaac tctgccacgg
    42841 cgtcggctgc cgataaactt tctatcaccg ccagtaatgc ggcagtgcag ggtggggagg
    42901 cgatgaccac ggtgatcaag acaatggacg atatcgccga cagtacccag cgcattggca
    42961 ccattacttc gctgattaac gatattgcgt ttcagaccaa tattctggcc ctgaatgcgg
    43021 cggtggaagc ggcgcgtgcc ggcgaacagg gcaaaggttt tgcagtggtg gcaggggaag
    43081 tgcgtcattt agccagccgc agcgctaatg ctgccaacga tattcgcaag ctgattgatg
    43141 ccagtgctga taaggtgcaa ttcggttcgc agcaggtaca cgccgccgga cggacgatgg
    43201 aagatattgt ggcacaggtg aaaaacgtca cccagttgat cgcccagatt agccattcaa
    43261 cgctggaaca ggccgatggg ctttccagcc tgacccgtgc agtggatgag cttaacctga
    43321 tcacccagaa aaatgccgag ctggtggaag agagtgcgca ggtgtcggcg atggtgaaac
    43381 accgcgccag ccgactggaa gacgcggtga cggtgctgca ttaatcggta tgccggatcc
    43441 ggcggttgga gcacaatgcc tgatgcgatg ctggcgcatc ttatcaggcc tgcggggtgt
    43501 gtagcgggtg taggccggat aaggcgcgtc aagcgtcgca tccggcaatt gcaccgcgcc
    43561 actggcggat gcggcgatta atccagttgc gtaatatcaa aagcggcgcg gtcgatcacc
    43621 gcaatgatct tttttatttg tgcatcactg atatcgctct ggttgacgcg taaatccagc
    43681 accgctttaa aattatccag cgcccgcttc atttgcgggt tctggcgcag cgccgcgcca
    43741 acgcaacgcg ctttgatgcg ttcttcaatc atctccacct gttcgcggtt ttcttccagc
    43801 cactgcgcgc cttgttcggt cagcgcaatc tgcttcttac caccttcctc ttcgcggatg
    43861 gtaatcagcg actgctcctg cagaaaatcc agcgtcgggt agatgacgcc cgggcttggg
    43921 gtgtaattcc cctgggttag attctcaatc gctttaatca attcgtaacc gtggctgtca
    43981 tcgcgcgaga gaatatccag aatcaccaga cgcaattcac cgtgaccaaa gaaacgttgc
    44041 cgacgaccgc cgccgcgacc gtggcgacca ccgcaacatt gaccgtgttc atgctggtga
    44101 ccgtgtccgc agtgctcgtg ttctgacttc tcacctttgc agcagccctc atggcgtggc
    44161 tggccttcat gtttacaaca cccttcgtga tgatggctca tatcagcctc cttttgcttg
    44221 attagatata tctaatttat atctgatgtg agcattactg caagtacaaa aatgattttt
    44281 aaacgtggaa gcacctcaca tctaggccgg ataagacgtt cacgccgcat ccggcagcat
    44341 tgctatggca ctgaatgcct tcacatactt cagacatatc agttctacaa atcgcttgca
    44401 tttatcatga ttaacaatca ttatcatttg cgaattttat ttagatatat ctgattacca
    44461 tcacgaaggc gataacaatg aataacaccc cccgctaccc gcagcgcgtt cgcaatgatc
    44521 tgcgcttccg tgaactgact gtgctacgcg ttgagcgcat cagcgccggt tttcagcgca
    44581 ttgtccttgg cggcgaggcg ttggacggtt ttacatcgcg cggctttgac gatcacagca
    44641 aactcttctt tcctcaacct gacgctcact ttgtgccgcc aacggtaacg gaagagggca
    44701 tcgtctggcc ggaaggacca cgcccaccgt cgcgtgacta tacgccactg tatgacgaac
    44761 tacgccatga actggcgatc gatttcttta ttcacgacgg tggggtcgcc agcggctggg
    44821 cgatgcaggc gcaaccgggc gataaactta cggtggcagg tccgcgcggt tcgctggtgg
    44881 tgccggaaga ttacgcgtat cagctgtatg tctgcgatga atccggaatg cccgcactgc
    44941 gccgccgcct ggaaacgttg agcaaacttg ccgttaaacc gcaagttagt gcgctggtta
    45001 gcgtgcggga taacgcctgt caggattatc tcgcgcacct tgatggtttt aatatcgaat
    45061 ggctggcaca tgatgagcag gcggtagatg cgcgtctggc gcagatgcaa atccctgccg
    45121 atgattactt catctggata accggcgaag gtaaagtcgt taagaattta agccgccgct
    45181 ttgaagcgga aaagtatgac ccacagcggg tacgtgcagc ggcttactgg cacgcaaagt
    45241 aactgatata taaaaagaaa aaggctgacg atttctcgtc agcctttgct atatctggtg
    45301 gcccctgctg gacttgaacc agcgaccaag cgattatgag tcgcctgctc taaccactga
    45361 gctaaggggc catggtagcg gattataaag taactccgtg tcgcaatcca gccattaccg
    45421 cgcgcctgct gtttttataa acaatgtatt ttcaatccgt tatacttttc ctggtgatgt
    45481 tagaaaggag taaatatggt tgaggatatt ttggctccag ggttacgggt cgtgttttgc
    45541 ggtatcaacc ctgggctttc atccgccggg actggttttc cctttgctca tccggcaaat
    45601 cgcttctgga aggtgatata tcaggccggg tttaccgacc gtcagttgaa gccgcaggag
    45661 gcacagcatc tgctggatta tcgttgtggc gtcaccaaac tggtagaccg tccaacggtg
    45721 caagccaatg aagtttcaaa gcaggagctg cacgcaggtg ggcgtaagct gattgaaaaa
    45781 attgaagatt atcagccgca ggcgttggcg attctgggca aacaagcata tgaacaggga
    45841 ttcagccagc gcggtgcaca gtgggggaaa caaacgctca ccattggttc gacgcagatt
    45901 tgggtgctgc caaatcccag cggtttaagt cgcgtttcac tggagaaact ggttgaagcg
    45961 tatcgcgagc tggaccaggc gctggtagtg cgtgggcgat aaaaaacgcc accga
//
LOCUS       DADLSA010000017        43486 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.017, whole genome shotgun sequence.
ACCESSION   DADLSA010000017 DADLSA010000000
VERSION     DADLSA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.017"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..674)
                     /locus_tag="JN691_000966"
     CDS             complement(<1..674)
                     /locus_tag="JN691_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAY7242470.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGGNNFAAQGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYR"
     gene            complement(729..1670)
                     /locus_tag="JN691_000967"
     CDS             complement(729..1670)
                     /locus_tag="JN691_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7242471.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            complement(1701..2675)
                     /locus_tag="JN691_000968"
     CDS             complement(1701..2675)
                     /locus_tag="JN691_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY7242472.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            complement(2672..4192)
                     /locus_tag="JN691_000969"
     CDS             complement(2672..4192)
                     /locus_tag="JN691_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7242473.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDDPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            4306..6570
                     /locus_tag="JN691_000970"
     CDS             4306..6570
                     /locus_tag="JN691_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAY7242474.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDNILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LADFNRELRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSGQRV
                     EQSYPDLRSGERLIMIFALLAVVITAFAGWYIYRNLGSNLTAISRAMSRLAHGEADVT
                     FPALQRRDELGELARAFNVFARNTASLERTSRLLKEKTTQMEIAQTERQGLEEALLHS
                     QKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNTNSPDAPRIQRAQQAAERGALLTQRL
                     LAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLTIEIEAQSPAWSAWIDVSQLENA
                     IINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGSGMSQAVKSQV
                     FEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAAKNL
                     SPAAVEQTAVNGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAASAE
                     IDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLRKPF
                     TRAQLAQVLGQIEG"
     gene            6651..7283
                     /locus_tag="JN691_000971"
     CDS             6651..7283
                     /locus_tag="JN691_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7242475.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRAAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIACESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGGCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            7352..7681
                     /locus_tag="JN691_000972"
     CDS             7352..7681
                     /locus_tag="JN691_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242476.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(7828..8586)
                     /gene="phnP"
                     /locus_tag="JN691_000973"
     CDS             complement(7828..8586)
                     /gene="phnP"
                     /locus_tag="JN691_000973"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HAY7242477.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHAQGLFPLRWGVGDPIPIYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLIENALPSGFEAGFDGMEIGVA"
     gene            complement(8588..9022)
                     /gene="phnO"
                     /locus_tag="JN691_000974"
     CDS             complement(8588..9022)
                     /gene="phnO"
                     /locus_tag="JN691_000974"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HAY7242478.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            complement(9009..9566)
                     /gene="phnN"
                     /locus_tag="JN691_000975"
     CDS             complement(9009..9566)
                     /gene="phnN"
                     /locus_tag="JN691_000975"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HAY7242479.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            complement(9566..10645)
                     /gene="phnM"
                     /locus_tag="JN691_000976"
     CDS             complement(9566..10645)
                     /gene="phnM"
                     /locus_tag="JN691_000976"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013573840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAY7242480.1"
                     /translation="MTHLYAPGKGFSDVSFDLWPGEVLGIVGKSGSGKTTLLKSISAR
                     LTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSAGGNIGERLMA
                     TGARHYGDIRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLMDHSPGQRQFANREKYR
                     EYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAALCRARQIALASHDDATHAHV
                     AESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVRGGSHSGNVAASELAQLGLL
                     DILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTQNPAQALNLQDRGVIGEGKRA
                     DLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            complement(10672..11517)
                     /gene="phnJ"
                     /locus_tag="JN691_000977"
     CDS             complement(10672..11517)
                     /gene="phnJ"
                     /locus_tag="JN691_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAY7242481.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            complement(11510..12574)
                     /gene="phnI"
                     /locus_tag="JN691_000978"
     CDS             complement(11510..12574)
                     /gene="phnI"
                     /locus_tag="JN691_000978"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HAY7242482.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(12574..13158)
                     /gene="phnH"
                     /locus_tag="JN691_000979"
     CDS             complement(12574..13158)
                     /gene="phnH"
                     /locus_tag="JN691_000979"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAY7242483.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLKALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(13155..13607)
                     /gene="phnG"
                     /locus_tag="JN691_000980"
     CDS             complement(13155..13607)
                     /gene="phnG"
                     /locus_tag="JN691_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAY7242484.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(13608..14333)
                     /gene="phnF"
                     /locus_tag="JN691_000981"
     CDS             complement(13608..14333)
                     /gene="phnF"
                     /locus_tag="JN691_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HAY7242485.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(14354..15132)
                     /gene="phnE"
                     /locus_tag="JN691_000982"
                     /pseudo
     CDS             complement(14354..15132)
                     /gene="phnE"
                     /locus_tag="JN691_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
     gene            complement(15238..16254)
                     /gene="phnD"
                     /locus_tag="JN691_000983"
     CDS             complement(15238..16254)
                     /gene="phnD"
                     /locus_tag="JN691_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7242486.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(16279..17067)
                     /gene="phnC"
                     /locus_tag="JN691_000984"
     CDS             complement(16279..17067)
                     /gene="phnC"
                     /locus_tag="JN691_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAY7242487.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALPGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(17200..17643)
                     /gene="yjdN"
                     /locus_tag="JN691_000985"
     CDS             complement(17200..17643)
                     /gene="yjdN"
                     /locus_tag="JN691_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAY7242488.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(17901..18236)
                     /locus_tag="JN691_000986"
     CDS             complement(17901..18236)
                     /locus_tag="JN691_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAY7242489.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            18637..20865
                     /gene="crfC"
                     /locus_tag="JN691_000987"
     CDS             18637..20865
                     /gene="crfC"
                     /locus_tag="JN691_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAY7242490.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            20862..21740
                     /locus_tag="JN691_000988"
     CDS             20862..21740
                     /locus_tag="JN691_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242491.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            22004..23506
                     /gene="proP"
                     /locus_tag="JN691_000989"
     CDS             22004..23506
                     /gene="proP"
                     /locus_tag="JN691_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAY7242492.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            23618..23707
                     /gene="pmrR"
                     /locus_tag="JN691_000990"
     CDS             23618..23707
                     /gene="pmrR"
                     /locus_tag="JN691_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAY7242493.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(23683..24783)
                     /gene="pmrB"
                     /locus_tag="JN691_000991"
     CDS             complement(23683..24783)
                     /gene="pmrB"
                     /locus_tag="JN691_000991"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAY7242494.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(24784..25452)
                     /gene="basR"
                     /locus_tag="JN691_000992"
     CDS             complement(24784..25452)
                     /gene="basR"
                     /locus_tag="JN691_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAY7242495.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTMRGFGYMLVA
                     NEEN"
     gene            complement(25449..27092)
                     /gene="eptA"
                     /locus_tag="JN691_000993"
     CDS             complement(25449..27092)
                     /gene="eptA"
                     /locus_tag="JN691_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAY7242496.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISIRLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(27196..28533)
                     /gene="adiC"
                     /locus_tag="JN691_000994"
     CDS             complement(27196..28533)
                     /gene="adiC"
                     /locus_tag="JN691_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAY7242497.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDRLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(28670..29431)
                     /locus_tag="JN691_000995"
     CDS             complement(28670..29431)
                     /locus_tag="JN691_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7242498.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(29756..32023)
                     /gene="adiA"
                     /locus_tag="JN691_000996"
     CDS             complement(29756..32023)
                     /gene="adiA"
                     /locus_tag="JN691_000996"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAY7242499.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDDAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(32222..33130)
                     /gene="melR"
                     /locus_tag="JN691_000997"
     CDS             complement(32222..33130)
                     /gene="melR"
                     /locus_tag="JN691_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HAY7242500.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            33413..34768
                     /gene="melA"
                     /locus_tag="JN691_000998"
     CDS             33413..34768
                     /gene="melA"
                     /locus_tag="JN691_000998"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAY7242501.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            34883..36292
                     /gene="melB"
                     /locus_tag="JN691_000999"
     CDS             34883..36292
                     /gene="melB"
                     /locus_tag="JN691_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAY7242502.1"
                     /translation="MTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLF
                     LVARIWDAINDPIMGWIVNATRSRWGKFKLWILIGTLANSVILFLLFSAHLFEGTTQI
                     VFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTL
                     PFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHLTLKA
                     IVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANL
                     VTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGT
                     ALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGY
                     VPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKV
                     PPEPVHADIPVGAVSDVKA"
     gene            complement(36431..37060)
                     /locus_tag="JN691_001000"
     CDS             complement(36431..37060)
                     /locus_tag="JN691_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAY7242503.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(37183..38829)
                     /locus_tag="JN691_001001"
     CDS             complement(37183..38829)
                     /locus_tag="JN691_001001"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAY7242504.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(38907..40247)
                     /locus_tag="JN691_001002"
     CDS             complement(38907..40247)
                     /locus_tag="JN691_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY7242505.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(40818..41537)
                     /gene="dcuR"
                     /locus_tag="JN691_001003"
     CDS             complement(40818..41537)
                     /gene="dcuR"
                     /locus_tag="JN691_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAY7242506.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(41534..43165)
                     /gene="dcuS"
                     /locus_tag="JN691_001004"
     CDS             complement(41534..43165)
                     /gene="dcuS"
                     /locus_tag="JN691_001004"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAY7242507.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            43346..>43486
                     /gene="yjdI"
                     /locus_tag="JN691_001005"
     CDS             43346..>43486
                     /gene="yjdI"
                     /locus_tag="JN691_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY7242508.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
BASE COUNT         9871 a        11222 c        11910 g        10483 t
ORIGIN      
        1 tggcggtatt cgttacacaa caccagctgg gccggttcgt cttcgtcaat caggttgtag
       61 cggtccagcg gttgtaaaaa gtggttatcg tggtcgtcgt cattgacaga agagacttcg
      121 ccaaccagaa catcgccaaa accggtttcc gcccaaaagc tgtgatacag gccgggcggc
      181 aggcagatac tttcgccggg ggacaggcgt aactgactgc ctgcgacatg agtctggcgg
      241 cagccgtcga tcaccaccgt aacgtcggta tcaactgttt gctcgttgct atcggcattc
      301 cacagttcaa caatcaaatt gccgccgccg cgattgatga tgtcctcacg cttacgccag
      361 tgaaaatgca ttggcgtgac ctgcgcatcg cgcacatgca tgatcttctc ggcgtaacat
      421 ttcacatacg gcattccttt tgctgagccg ttacgcaggg taaacagcgt cagcccctgt
      481 gctgcaaagt tgttaccacc aaaagcggta acatcccagc cgagtttgag atcaaaaacc
      541 tcctcccacg cggcggtgtc gagctgctgc cattgagcag gagagaaact ggcaaagggc
      601 ggcagatgta cgtcatgttg cgaaaagaat tgccgtgtat ggccgagaat gttgtttata
      661 gcggagcgtt tcatcgaaag cgttctcctt agcggtggcc gggcagcatc gccgcccggc
      721 gtgtggactt atttcaccgt ccagcctttg taattgccga cgttgttttt atcgatcatc
      781 gtcaccggga tcagcaccgg ctctttcggc gcaggtttgc cctgcaaaat gtcataaccg
      841 atctcaaccg ctttcgctgc catcacctgt ggatcttgcg ccggagtagc gacaaacaac
      901 gagttctcac gcttgagcgc ctcttcggcg tccgggcttc catccacgcc gacaataaag
      961 aactcgttac gttgcgcctg ttttgctgcc agatcggcac cgatcgctgt cggatcgtta
     1021 atcgcaaata cgccgtcgat cttcggattt gccgccagca gcccggtcat cacctccagc
     1081 ccgccctcac gactgccttt ggcgttcttg ttgtcagaga gcaccttgat gtcaggatgg
     1141 cgtttaaatt cggtctggca gccttcgacg cggttttgca cggcggacac cggcggtccg
     1201 ttgatgatca ccacgttgcc tttgcctttc aggcgatcgg tgatgtattt acaggccatt
     1261 tcgcctgcct gggtgttatc ggaagtgatg gtggcatcgg caccttctgc cgccacgtca
     1321 accgccacca ccacgatccc ggcgtctttt gcccgtttca ccgccgggcc gatccctttt
     1381 gaatcggcag cgttaaggat gatcatatcg acgttagcgg cgataaagtt gtctatctgc
     1441 gagacctgct gacccagatc gtagccgctg gaaaccagcg tcactttgac gttatcgccc
     1501 gccagtttgc gtgcttccag ctccgcgcct ttggtgatct gtacgaagaa cgggttcgcc
     1561 aggtcaccta ccgttacgcc tatcgatttc agatctttcg cctgggcgaa cggagctgcg
     1621 gccagcagcg cgcctgcaca gagcgcggtc actaagggct tcaatctcat gctgtattcc
     1681 tcatgtgtag ggtattgttg ttatgcactt tgatgatgtc gggtacggta tttgtcgata
     1741 agcaccgcaa tgatgatcac cgcccctttg atcaccagct gccagaagta agagacgccc
     1801 attaaggtca tgccattgtt gagcgtggcg ataattagcg cccccaccag cgtgccggtg
     1861 atggtgccga tgccgccgac aaaactggtg ccgccgagga tcaccgccgc aatggcatcc
     1921 agttcgtaac ccatacctaa gttgccgttg gcgctgtaca gccgcgaggt gctcataatc
     1981 ccgccaagcc ctgaaagcag gccgctcatg ccataaacaa acagcagcac cagccagacc
     2041 ttaatgccgg taagacgcgc cgcctgcata ttgccaccga cggcgtagat atgaacgcca
     2101 agcgtggtgc gacgcagaat aaaccagcac agcacgatca ccgccagcgc aatcaccacc
     2161 agccacggga ccggcccgag atagttattg cctatccact cgaaattaat gttggagttg
     2221 ataaccgtgg tgccatcagc cagcagatac gccgccccgc gcagcgccgt ataggtgcca
     2281 agcgtgacga taaacggcgg caatccggca aaggccacca gcgcgccgtt gaacagcccc
     2341 agcagcaggc cgagcatcag cgccgccggg atagacaaca tggcgaactc cgggctaagg
     2401 gagacaacca ttgccgccac cgccgtggtg ccgaggattg aacctacaga aagatcgatc
     2461 ccaccggtca aaatgatgaa ggtcatcccc gccgccagca caatgttgat cgacgcctgg
     2521 cgggtaatat ttagcaggtt gctttcggta aaaaagtttg gcgcgataaa gccaaatacc
     2581 gctacgatca ggatcaaaat gggcaaaatg ccgaccgttt gcatcagatc gctcatcagc
     2641 atttttttgg cagaggtttt tgcagaggtc gtcatgaggt caccgcctgg gaatgggcgt
     2701 cgttgacgcc agtcgctaag gtcataatgt tttcctgggt gatgtttttg ccatttagct
     2761 caccggcaat gctgccttcg cgcatcacat ggacgcgatc gctcattccc actatctccg
     2821 gcagttcgct ggagatcatc aggatcgcca cgcccttgcg cgccatctcg ttcatgatcc
     2881 ggtagatctc actttttgcg ccaacgtcca cgccgcgggt gggatcatcg agcagtaaaa
     2941 tgcgcgggcc aatcgccacc cagcgggaga tcaacagttt ttgctgattg ccgccggaaa
     3001 gcccgcccgc gcgtacctgg gcatgaggca cgcgaatgtt gagcaactta atggcgtcat
     3061 cggaaatggt ttgcgctttt ttgcgattga gcattcccca gctggcatcg cgctccaggg
     3121 ttgccatagt gatgttttcg actgccgcca tttcaaggaa taagccttgt tctttgcggt
     3181 tttcggtgag aaaaccaatg ccaagatcga tggcttcgcg cggggagtgg atcaccaccg
     3241 gttcgccatc gacttcaatc attccgcctg ttgctttccg cacgccgaaa attaactgcg
     3301 ccagttcgga acgtccggct cccaccagcc cggcgaggcc aacaatttcg ccggaaagca
     3361 cctgcaggca gctcggctgg actttgccgc cgtcggtcag gtggtgaaca ttcaggcggg
     3421 ctttaccgag cgggatatcg cgctctttat tgaacagatc gctcagtggc ctgccgacca
     3481 tcatccgcac cagctccccg gcattgaggt tatcgcgggt caggctgcca acgtattgcc
     3541 cgtcgcgcag cacgctgacg cgatcggaaa gctcatacac ttccgccata cggtggctga
     3601 tgtagataat cgccatccct tcatcgcgca gccgcataat cagttcaaaa agacggtgag
     3661 tttcacggga ggagagggca gcggtgggtt cgtccatcac cagaatgcgg ctgttgcgat
     3721 gtagcgcgcg ggcgatttcg acctgctgtt gttcggcaat ggtcagcgtc atgaccttat
     3781 cgctggcgtt aaactgcgcg ccgaggcggt cgatcactct ttgcgcctgc accagcatct
     3841 cttttcgctg caccagcccg ccgtgggaaa gctcgctgcc gaggaaaata ttttctgcca
     3901 ccgtcaaatt gggtgccagc tgcatctcct gataaatcag cgtaataccg gcgcttaagg
     3961 catcttttgg cgtgcgaatg tgaaagggct tgccgtctat caggatctcg ccgctggtgg
     4021 cggtatacgc ccccgccagc accttcatca gcgtgctttt tcccgcgccg ttttccccca
     4081 tcagggcatg gatctcgcca gggtagaccg tcaaatccac ccctttgagc gcataaaatt
     4141 tgccgaaggc tttggcaata ttgcgcatct caagaaccgg cgtgctgttc atggtggact
     4201 cctgcgaggt tgtcgatatc agcactccat cacaggaaag taaatgcaaa atgtcagaag
     4261 ccgttcatat ttgtcatatt gcgatttttc ttcacggagc gtgatatggc gcaccccgga
     4321 cgtcactttt ttgccagcgc gcgcgggcga ttgctgcttt tgaatttgct ggtggtggcg
     4381 gtgacgttga tggtcagcgg cgtggcggta atgggctttc gtcatgccag ccagatgcaa
     4441 gagctggtgc agcagcaaac tgtagatgat atgaccggca gcctgaatct ggcgcgcgac
     4501 acggcaaacg tggcaacggc ggcagtgcga ttgtcgcagg tggtgggagc actggaatat
     4561 aaaggcgaag ccgagcggtt gcaggagaca cagcgggcat taaaaagctc gcttgcacaa
     4621 ctggcgaacg cgcctttagc gcagcaggaa gccgggctgg tgacgcgcat catcacgcgt
     4681 agcaacgaac tgcaaaccag cgtcggcgga atgctggaac gcgggcaaag gcggcatctg
     4741 gagcgtaacg cgctgttaag ttcgctgtat caaaacttaa gttatctgcg gcatctgcaa
     4801 aaagtcactc acgcgcaaga taatattttg cttaacgaga tgaaccgact gattgttgcc
     4861 gccattgcca cccccgcgcc gcaggcgatt attcatcaac tggtcggagt gatgtctgcc
     4921 ttgccaactc acagcgatac gccattggtt aatacgctac tcgctgattt taatcgtgaa
     4981 ctgcgtaaac tcgcgccact ttcggcggct ctggagcaaa gcgatctggc aatcagctgg
     5041 tatatgtttc acatcaaagc gctggtggcg atccttaatg gcgacatcaa ccgttacgcc
     5101 gaacaggtgg cgacgctctc tggtcagcgg gtggagcaaa gttacccgga tttgcgctcc
     5161 ggcgagaggc tgatcatgat tttcgccctg ttagcggtgg tgatcaccgc gtttgcaggt
     5221 tggtatatct atcgcaatct cggctctaac ctgacggcga tttcccgcgc gatgagccgc
     5281 ctggcgcacg gcgaagcgga cgtcactttt ccggcgttac agcggcgcga tgagttgggt
     5341 gaactggcgc gcgcctttaa cgtttttgcc cgcaataccg catcacttga acgaacgtca
     5401 cggctgttga aagagaaaac cacgcagatg gagattgccc aaaccgagcg gcaggggctg
     5461 gaagaggcgc tattgcacag ccagaagtta aaagcggtcg ggcaactgac gggcgggctg
     5521 gcccatgatt ttaataatct gctggcggta attattggta gcctggaact ggtgaacacg
     5581 aactcacccg acgcgccgcg aatacagcgg gcgcagcaag ccgccgaacg tggcgcgcta
     5641 ctgacccagc gattgttggc attttcccgc aagcagtcct tacatccgca agccgttgaa
     5701 ctaaaaacac tgctggaaga gttaagcgaa ttaatgcgcc actccttacc ggcgacgctg
     5761 acgattgaga ttgaagcgca gtctccggcg tggtccgcgt ggattgacgt cagccaactg
     5821 gaaaacgcca tcatcaacct ggtgatgaac gcccgcgacg cgatggaagg gcagacggga
     5881 attatcaaaa ttcgtacctg gaatcagcgt gttacccgca gcagtgggca gcgtcaggac
     5941 atggtggcac tggaggtcat cgatcatggc agcggtatgt cgcaggcggt gaaaagccag
     6001 gtgttcgagc cgttctttac caccaaacag acggggagcg gcagcgggct gggcttgtcg
     6061 atggtctatg gtttcgtgcg ccagtccggc gggcgcgtgg aaattgaaag cgcgccgggg
     6121 caggggacaa cggtcagatt acagcttccc cgcgcgctga cggcggcgaa aaacctatcg
     6181 ccagcggcgg tagaacagac agctgttaat ggcgataaac tggtgttagt gctggaagat
     6241 gaagccgcgg tgcgccagac catctgcgag cagttgcacc tgctgggcta tttgacactg
     6301 gaagcgagca gcggcgaaca ggcgctggac ctgctggcgg catcagcaga aatcgatatc
     6361 tttatcagcg acttaatgct acctggcggc atgagcggcg cagaagtggt caatgcggcc
     6421 cgcaaacttt acccgcacct gacgctgcta ctcatcagcg gtcaggattt acgccccagc
     6481 cataaccccg cgctgccgga cgtggcgctg ctgcgcaaac cctttacccg cgcgcagctg
     6541 gcgcaggtgc tggggcaaat tgagggatga ttttcagaat ttatcaagag gctcgaagca
     6601 aacgtgctgc tattgtgagc aattctgttc tgaattgaag tgatttacat atgatcgtga
     6661 tgccaaccac ctatagccct atgacggttg ctcgttcttt tcgcgtggtg gaagagatcg
     6721 agctttctgg acgtctgtta caggtaattt atgacaacca gacgccaaga gcggccatca
     6781 ttgaagcgga cattgaagtt tttgcaggcg tgccgcgtca tcgggtactt gccgctctcg
     6841 atctgcagcg taaaaaagag ttcggcagcg atatcattgc cgttgaacgc tggtgggaag
     6901 actcggatct ggtacaggtg gaaggtattt gtgtctctcc aggtgagcgc gataagggtc
     6961 tggcaactcg gctgtatgaa gcgctaatcc tgcaatgcgg tatcacgcta atcagcgatt
     7021 ttgatcaata tgatggcggg aagatgctct ggcagaagat cgcgtgcgag tccgatcaga
     7081 tttccgtgtt tgtattagac actgaacaag gggcattttg gccctacgat ggcacgaaag
     7141 tgatctacga tggcggctgc attccggaag agcgtatctg gagtatttca ccggatcaaa
     7201 aatgtcatgg cattgtattg gtggcggaaa ataaacagcg agctaaccag ctaatggaag
     7261 agtctgcgcc ttaccgaatt tgagattact ccgctactcc ggtcattcac actcaactaa
     7321 cgttaagcca gtccatcgca gagactggct tatgaaacct tactccacgc tttttctttt
     7381 caccctgctc acgctgtcca ccgttccggc ccaggccgat attatcgacg atactattgg
     7441 taatattcag caggcgatta atgacgctta taactctgac cgtgggcgag actatgaaga
     7501 ttcgcgcgac gacggctggc agcgtgaagt gagcgacgat cggcgcagac aatatgacga
     7561 ccgccgccgc cagtttgaag atcgccgcag gcagctggac gatcgccagc gccagcttga
     7621 tcaggagcgt cggcagctag aagatgaaga gcggagaatg gaagatgagt atgggcgatg
     7681 aggttgggta tggagcaggc atcgcccctc accccaatgg ggagagggag aaaaccagcg
     7741 caatattcaa tatcgagcac aatcgctccc ctcgcccctc tggggagagg gttagggtga
     7801 gggggccagc gcgcacattc cctctcatca cgccacccca atctccatcc catcaaaccc
     7861 cgcctcaaac cccgacggca gtgcgttttc tatcagccac gcatcaaact ggtggctgat
     7921 atgggtcaga atcacccgtg gcgagcggat aacctgattc agcgcaagca cggtatttaa
     7981 atcacagtga ttacgcggcg catccgcgcg cggcgggtgg ctgcaatcaa tcaccattac
     8041 ctgtggctga ttattgcgta aaaatttcag cgttttttcc ggcaggccag cggtgtcaga
     8101 tagccacgcc acccggctat gtgccgtttc cagcagataa ccgaaggtca gttttgagtg
     8161 gttaagcggc agcggcgtga cctgcaaccc ctgcaaatca aacaccacaa acggttccac
     8221 tgtgtggctg aaatcaagca ggcccggatg tttaaacaga tcgtcgcaac cctggtcatc
     8281 cggcgggccg tagatcggga tcggatcgcc cacgccccag cgcagcggaa acagcccctg
     8341 ggcgtgatcc atatgataat gcgtcagcaa aaactgctgg aacgatccgg gcgaccagcg
     8401 atcggcgaga tcgtgcagcc cggcgtcgat cagggtgatt gcgtcgttaa acttcactac
     8461 gccgctgcat ggctggcggc gatactgcgg tgagcgccgc gctcggtcgc aggccgcgca
     8521 ctcgcagccc catgccggaa cgccctgtgc gccgccagtg ccggtgagcg tgagggtcag
     8581 gctcatgtta cagcaccttg gtgaagcgga agtgactctg ctcgtagcct tctcgcagat
     8641 agaaacggtg cgcgtcgtgg cgtttcacgt tggtcgaaag cgcggtcatt tcggccccgg
     8701 cctggcgggc ttcttcttct gcccatgcca gtaatttact gccgacgttc agaccgcgcg
     8761 cctgcggcat caccaccagt tcctgaattt cgccgatcca gttgacatga tgcagatgaa
     8821 actgcaaatg caggccgatc atgctgacaa cttcgccatc cagcagcgcc agatggtagc
     8881 gcatgtttgg gtcgcgcagg ttggcgttaa agcccacgcg aaacgcatgg tggtcaaact
     8941 ccgcctgttt tagctcacaa atcagcgcgt aaactgcgtc ggtgtcgtac tgcgtagccg
     9001 ggcgaagctc acaagcaggc atggtgtttc tccttctgat gaatcagcgt cagcagcttg
     9061 tcgaccgact ggcgcaggct gccgtcatta ttgagcgtaa gacaatcctg aggagtatag
     9121 cgggcagcgc gtgctagtcg ggcgttaatt tcactggcat tttcacgacc acggttttcg
     9181 aggcgctggc gcaggatctc cggcgaaacc tgtaagcaaa tgggcagcag cgccgattga
     9241 tagcgcgccc gcgcctgcgg cagatgggcg cgtgagccgt tgaccagcac gtcgaatccg
     9301 gcgtgcagcc agagatcaat ctcaacgccg acgccataat acagactgtt agcgtgccag
     9361 cttaaggcca acagattttg ccccgcgcgg gtaaaaaact cctgctcgct cagggcgata
     9421 tggttttcac ttccggcgct ggcgtcgcgc gtaatgtagc gatgcgccac cagtaactga
     9481 gtttgttccc gcaggcggat ttccgccagc aggctgtctt tcccggagcc ggacggcccc
     9541 attaaccaaa tcagttttcc catcatcaga acaccctttt accctgacgc cagacgtggt
     9601 cgatatgaat atgattgtcc ttgcgatgcg ccagcaccag gtccgcgcgt ttgccctcgc
     9661 caatcacccc gcgatcctga agattaagcg cctgcgccgg attttgggtc actaacttca
     9721 ccgcctgcgg cagcgtaaag cggttgctct ggtcatcggc gacgcgaaat gccgcatcca
     9781 gcaggctggc ggggtagtag tcggaagaga ggatatccag caggccaagc tgcgccagtt
     9841 cactggccgc cacgttgccg gagtgcgaac cgccgcgcac gatattcggc gcgcccatca
     9901 gtacgttcat gccatgcttg cgcgaggctt ccgccgcttc aaacgtggtg ggaaattcgg
     9961 cgatcacgct gccaagcagg tgggattcgg caacgtgggc gtgggtggcg tcatcatggc
    10021 tggcgagcgc aatttgtcgc gcgcggcaca gggcggcgat ggattcgcga ttcggctgcg
    10081 accagcgtgc ggcaagcgcc agttgctctt cttcgtactg ctgcatctgc gcatcggtga
    10141 gcgagtattt gccctgataa tattcgcgat acttctcgcg gttggcgaac tggcgctggc
    10201 ccggcgagtg gtccatcagc gtcaccggct cgcgctgcac cagtttttca aacagcggca
    10261 gcgtggtgtg atgcggcagt tcgcagcgca ggtgcagacg gtgctcggcg cggatgtcgc
    10321 cgtaatgacg ggccccggtc gccatcagcc gctcgccgat attgccgcct gccgagacct
    10381 ggcgacgcag gccgtcgagt ggatgctgat gcaccacgcc ccattcggtg cgcagcaggc
    10441 gacggcggtc ggcctcgctc atcgcataca acgaacgatt ctcgtagcga atctccccct
    10501 gctgcggcgt caggcgcgcg gagattgatt tcagcagcgt ggtcttcccg gagccggatt
    10561 tcccgacgat gcccagcact tcccccggcc ataaatcaaa agagacatcg ctaaagcctt
    10621 tgcccggcgc gtaaaggtgg gtcaggttat tgaccgaaag taacggttga ttcattggtt
    10681 ttttgcctcg ctctgttggc ggcaataatc ggtatcggag cagacaaaca tgcggtttcc
    10741 ggcgtcatcc agcaccactt cgtcgagata gctgtgggtc gatccgcaga tggcgcatgg
    10801 ctcatcccac tgctgaacgg tgaacgggtg atcgtcgaaa tcgagacttt ccacgcgggt
    10861 aaacggcggc accgcataga tgcgcttctc gcgccctgcg ccgaacagct gcagggcggg
    10921 catcatgtcc attttcgggt tatcgaattt cgggatcggc gacgggtcca tcacgtagcg
    10981 cccgttcacc ttcaccggat aagcataggt ggtggcgata tgaccgaagc gggcgatatc
    11041 ttcatacagt ttcacctgca tcacgccgta ctcctccagc gcgtgcatag tgcgggtttc
    11101 cgtttcgcgc ggctcgataa agcgcagcgg ttcggggatc ggcacctgga aaataactat
    11161 ctgatcttcg gtcagcggcg tttcggggat gcgatggcgc gtctggataa gcgtcgcatc
    11221 gtccgtacgt tcagtggtgt tcaccccggt cacgcgcttg aagaagttgc gaatcgacac
    11281 ggcgttagtg gtgtcgtctg cgccctggtc aatcaccttc aacacgtcgc tttcgccaat
    11341 cacgctggcg gtgagctgaa tgccgccggt tccccagccg tagggcatcg gcatctcgcg
    11401 cccgccaaac ggcacctgat aaccggggat agccaccgct tttaagatgg cgcggcggat
    11461 catgcgtttg gtctgctcgt cgaggtaggc aaagttgtag ccgctcagat tagccatggt
    11521 tctgctcctg ttgcagacgt ttgagtagct ccagttcggc ctggaaatcg acgtagtggg
    11581 ggagtttgag gtgcgagaca aagcctgcgg cttcgacgtt gtcggcatgc gccagcacga
    11641 actcttcatc ctgcgccggg cctgtcgcgt gctcgccgta ttccggggct tgcaacgcac
    11701 ggtcaaccag cgccattgcc atcgctttgc gctcgctcat gccgaatacc agcccgtagc
    11761 cgcgcgtgaa gtgcggcggc tcatccggcg ggtcgataaa accgttgacc atttcacact
    11821 cggtcatcag cagttcgccg acgtttaccg caaatcccag ctcttccggc acaatcgaca
    11881 cgtcgatgta accactgcgg atctcgcccg cgaacgggtg attgcgtccg taaccacgct
    11941 gggtggagta ggccagcgcc agcaaatagc cttcgtcccc gcgcattaat tgttgcaggc
    12001 gggaggagcg tgagcagggg taaaccggcg gcgtgcgggt gatgtcatcc ggctgtgcgc
    12061 cgctatcttc ttcaaacttc gccagcccct gacgcgccag caggctgaaa acgtgcggcg
    12121 acggctgttg ttcgctgtcg gcggtggtca gcgtcggcgc ttcaccgttt gccagcaggg
    12181 tgaaatcgag caggcgatgg gtgtagtcgt aggttgggcc aagcagctgg ccgccgggaa
    12241 tgtctttata aaccgccgaa atacgccgtt cgagacgcat ctcggtggtg tcgagcggct
    12301 cgcttaccgc cagtttcgcc agcgtggtgc ggtaggcgcg cagcaggaaa attgcttcaa
    12361 cgttatcgcc gctggcctgt ttcagcgcca gcgccgccag ttcgcggtcg gcaacgccgc
    12421 cttcggtcat cacgcgatcc accgcgagat taagctgctg ttcaatttgg gcgacgctca
    12481 gttcgggcaa atcggtatcg ccccggcgtc ggctctcttg cagggcgtgg gcggcgtcga
    12541 tcgccttttc gccccctttc acggcaacgt acatcagcac acctccacat gagtggttcg
    12601 cggaatagcc agcaggcgtt cgccgcaggt caggatcagg tcgatgccga gcgggaacgg
    12661 gtgcgggcgc tcggtgagtt cgtgcagaat gcactccggc agctgcggag cgatcattcg
    12721 ttcttcggca atacccgcgc cggtgagacg caacatgcgc ccgccgctca ggctggcgac
    12781 ctgtaaaatc agcgtcgcgc ccgcttccgg cgcaacggcg gtgccggtgg aaagggcttt
    12841 gagctgtttg ctggaaattc cgtcatccgt caccgcgaag gtcgcctgtt ccggctggct
    12901 gaccagcggc gcgttggtat gaaaacgcag gctctgattg acgatatcgt tactcaatgg
    12961 cgcggcaagc cacaccggcg tgtcgttatc agccagcgtc agcagcacgc tggtggtggc
    13021 gatattcagc ggctgccagc cgcgcttgag ctgatgcagg gcgacaatca cgcccggctc
    13081 gcttatggcc tttaacaggc gacgaaaact gtgctgggca tcctgcacgg gaagcataaa
    13141 agcggtttcc agggtcatgc gttgtctccg cgaaccatcg taaagaagtc gacccggctg
    13201 gcgttcactt cggcctggcg tgcggcaatg cgtgccatac ggtcagcgtc cagcggggca
    13261 ataagggttt ctgataagtt ttgaaagtgg cgagattgct gcatcagcgc atcaatcagc
    13321 gcgcagcgtt cggtgtgctg tttatcacgc cccagcaccc agctgtagcc gagggtgccg
    13381 tcagtcaggc gtacggcggc gcgggtcagc gtggcgtcgc cggcaaaaaa gcgttcgccg
    13441 gtgccgccca ttcgcgcctg aatctgtacc aggccggttt cggcggtgcg gatcacttca
    13501 tagtcggcgg tgatgttcag cgcgttcagg cgggctgcca gttcagccgg ttggctgtgc
    13561 gccagtacgg acatccagtg ctggcgggtc gcggtatctg cgtgcattca gtgctccata
    13621 gtgaattcaa tcatgtcggc gcgcgtcagg ctgacggagt actccgccgg gctgctttca
    13681 ccgtcacggt ggttaagggt gcgcacgcac agcagcggcg acatattcgg gatttcaagg
    13741 cgctggcact ctttggcctg ggcgcggcgg gcgctgatcc gcgtctggct gcggcgcagc
    13801 gcaattccgg tttgctcgcg cagaaaatcg tgcagcgaac cgctgtcgaa gcgttgcagc
    13861 gtcggccaaa gggtgaggtc cgcaaagtag tggtcgatta aacagagcgc gacgccgttg
    13921 acccggcgca gggtgcgcaa atggataacg ttctcgccct cggtaatccc cagcgcgtca
    13981 gcaacgtgac cggacgcggg gcgcaatacc gacagcagtt tttcgctggt gggatggctg
    14041 ccctggtcca gcaggttctg gctaaaacgc gcctgggcgt tgagcgggta atcgaacggg
    14101 cgcatcagca ccagcacgcc gacgccctga cggcgctgta cccagccttt ttccaccagt
    14161 tggtcgatgg cgcggcgcag ggtgtggcgg ttcacctcaa agcgcgccgc cagttgctgc
    14221 tcggcgggaa gatagtcgcc gcagcggtag tgttgacgaa gctcctgctc aagttttgcg
    14281 gctatctcct gatagcgtgt tgggtagctg gtcggatgtg tagacaagtg catagatatc
    14341 aatgcctcgc ttatcagata aagtgcttac gcaaccgttg agagaggaaa tccagcaggc
    14401 tgaccgtgac gatgataagc accatcaggg cgcaggtttg ctggaactgg aaaccgcgaa
    14461 tcgcttccca cagggtgacg ccgatcccgc ccgcgccgac catgccgacg acggtcgccg
    14521 agcggacgtt ggattcgaag cgatgaggga gtaggagatc agcagcggca tcacctgcgg
    14581 cagcacgccg taaaggatct cttcgagttt gttggcgccg gtggcgcgaa tgccttccac
    14641 cgggccgggt tcgatcgctt ccaccgcttc ggaaagcagc ttggagagca cgccggtggt
    14701 gtggataaac agcgccagca cgccagcgaa cgggccgagg ccgacggcga ccacgaacag
    14761 catggcgaag accatttcgt taatggcgcg gcaggcgtcc atcaggcggc gaacgggctg
    14821 gtaaacccac cacggcacca agttttcggc gctcatcaga ccgaagggga tggagagaac
    14881 cactgccagc gcggtgcccc agacggcgat ttgcagcgtg acggccattt cggtgaggta
    14941 atcctgccac tggctgaaat cgggcgggaa gaagtcggca gcgaaggttg ccatgttgcc
    15001 gccgtcttta atcagcgtaa gcggggccat ttccgcgccc tgccacgaga cgaccagcac
    15061 ggcgagaaca acggcccagc tcagaagcga gaaccagcta cgcttgggtg gggcgatggt
    15121 gatggtttgc atgtttggct ccggtctgta ggccggataa ggtgcttgca ccgcatccgg
    15181 catcaacgcc tgcacattgt cggatgcgac gccggggcgt cttatccgac ctacgattta
    15241 ctgcaccgct ttactcaccg aactcatcgc gcttaacgcg ttgttcaggc ggtccaggtc
    15301 atccagctgc gcctgaatct cggtggtttt cgccagcttg tcctgctcat tcagcccttt
    15361 attgcctttc acgctttgta tctctttaaa cagtgcgagc tggcgaatcg gcaccagttg
    15421 caggtcgctg gaagcgcgga acggcgccca gcccaggcgt tccagcaccg ctttctcttc
    15481 cggcgttttt ccgtagttca taaagaagtc gtagatcttg tctttggtag tttcggacag
    15541 attcttgcgc cagacgatcg gatcgcctag gatcagcggc gatttccaga tcaccttcag
    15601 ctctttaagc ttctccggcg cggaggtttt cagcttgtcg aggttttcgg tgttgttggt
    15661 ggcaacatcc acctgcttgt tggcgacggc cagcgcgttg gtttcatgcc cggcgttgac
    15721 ggtgcgcttg aagtcgctgg cggagatatt gtttttagcg aagacgtagt aaccggggac
    15781 gaggaagcca gaggtggagt taggatcgcc attgccgaag gtgagatctt tccgcttcgc
    15841 cagcagatcg ttcaggttgt tgatcgggct gtctttgttg acgatcaaca cgctccagta
    15901 acccggcgat ccatccgccg cgaccgtctg ggcgaagacc tggccattgg cgcgatccac
    15961 cgcttccatc gccgacaggt tgccgtacca ggcaatatcc actttattga agcgcatccc
    16021 ctggataatg cccgcgtagt ccggggcaaa gaaggcgttg accttcacgc ccagtttctt
    16081 ctccatatcc tgtaaaaacg gcgtccattg cggtttcagg ttttgctgtg attccgttga
    16141 aataatgccg aaattcagcg ccttttcctg ctcttcggcg tgtgccgggg ttaacagggt
    16201 gctgaggctg aacatgctgg tgaaggccag cgaggcaatt atcttagcgt tcattcgttt
    16261 tcctcaatga tggggatatc aggcagcttt cgcgttctct tcgatgcggt taatgctgcg
    16321 gtagagatgg tcaaaacgtt cgttatcaaa cagttggctg ctgccgtcgt agaagacgtg
    16381 cccctgacgc agggcgacga tgcgttcgca gtagcgcagg gcgtaatcca cctgatgcag
    16441 cgtgacgacc acggtgatgc cgtcgttctg gttgatgtcg cgcagggtgt ccatcacgat
    16501 gcgcgctgat tccgggtcca gcgaggcgat gggttcatcg gccagaatca ctttcgcctg
    16561 ctgcatcagc gcacgggcaa tcgccacacg ttgctgctgg ccgccggaga gggtggaaac
    16621 gcgctgatgg gcaaaatgca ccatgccaac gcgggtcagc gcctgtaacg cgcgctgttt
    16681 ctgctcgccg gtgaaccagc taaaacaggt gcgccagaac ggcgtgctgc cgagcgcgcc
    16741 aatcagcacg ttctccagta cgctcaggcg gttcaccagg ttgaattgtt gaaagatgta
    16801 gccggtgttg gcgcggcttt tgcggatatc gcgggccagg cggccttcgc gctggacagt
    16861 gcggcccagt agctcgatat ggctaccggc ggatttatcg ccggtaatca aaccgcttaa
    16921 atgacgtaaa agggtggatt tgccggaacc cgacggccca ggcagagcca ccatttcact
    16981 gtgatgaatg ttcagatcaa ccgcatgcag cgcttgatgc tgattgaagg ttttggcgag
    17041 cttctcgaca cggataatcg tttgcatgat gcagcctccc taaaaaagtg gctccatcgt
    17101 ggcggattat tgtgacgatt tggttaatta aaagtgacta acagatgaag agttaacggg
    17161 aattcgatga cagtgcggga gggcgggcct cccggcgggt tattgcgttg gttgttgttt
    17221 gacgacatta atcatccacg gtacgccaaa tttatcggtg actttgccaa agccatgcgc
    17281 ccagaaagtt tcctgccagg ccatttcgat ttttccgtta gcggcaaggt tatcaaacca
    17341 gcgttttcct tcttcgacct gttgcgaatc gagcaccagc gtaaagccgg agtagctggc
    17401 ttttcctgac ggtatagcat cgctcatcat gatgtcgctt ccggcaatgc gcacgttggc
    17461 atgagcgatg gcggtatcgg gaaattgcat tccggaaggg cagttctcgg cgctgtcctg
    17521 cgctgatttc ggcatttcgc cgaagctgat tttatagagc agttccgcgc ccaacgtacg
    17581 ttgataatag gcaatcgcgt cggaacagtt accggcaaaa gagaggtagg gacttaacgg
    17641 catgatggtg acctcagtta agagaagcca gttaagtgta gttcgaaatt tatacagatg
    17701 agaggctgcc tgataagacg cgcaagcgtc gcatcaggca gtcggcactg ttgccggatg
    17761 cggcgtaaac gccttatccg gcctacggag ggtgcgggaa tttgtaggcc tgataagacg
    17821 cgcaagcgtc gcatcaggca gtcggcatcg ttgccggatg cggcgcgagc gccttatccg
    17881 gcttatcgat cacaatacaa tcagttcttt ttcacaaact cagatttcag tttcatcgga
    17941 ccaaaaccgt cgattttgca atcgatgtta tggtcgcctt caaccaggcg gatgtttttc
    18001 actttggtgc caattttcag catcgaagag ctacctttca ccttcagatc tttaatgatg
    18061 gtaacgctgt cgccgtcagc cagcagattg ccgttagcat ctttaacgat cagctcgtcg
    18121 ctttcctgtg caggttctgc gtcgttccat tcgtaggcac attccgggca gatgtacatg
    18181 ccgttatctt cgtaagtgta ttcggagttg cattttgggc agtgtggtaa tgacatgtgg
    18241 atttcctcaa aagtcagcag ggcaaaagcg ccaaaaaacg gcagattgcc gaaaaaggcc
    18301 gcaattatac acaaaatccc tgagtttgtc gggggtactg ctgattttag acgacgatcg
    18361 ttttcatttg aaaggaaacg caaaaagaaa ataatctttt tgcctctatt tattatttaa
    18421 gataatgtta atgctctact tgttcttttt tttattcaca tgatattttt atctttctga
    18481 cgttttaaac gcatcagaag acgttattta ttctgataaa aaaaacacac taaatataaa
    18541 attatttctt gcagtaatta taaaagccga aaacagaact caaaaacaat ctggctaaat
    18601 aaaagtaaca aaatttgctt taaggaagaa ttttctatgt acacacagac cctgtatgag
    18661 ttaagtcagg aggctgaacg cctgttacag ctttctcgcc aacagttgca gttactggaa
    18721 aaaatgcctc tctctgtacc cggagacgac gcgccacaac tggctttacc ctggagtcag
    18781 cctaatatcg ccgaacgtca cgcgatgctg aataatgagt tgcgtaaaat ttcccgactg
    18841 gaaatggtgc tggcaattgt cggtaccatg aaagcaggga aatcaaccac cattaatgcc
    18901 attgttggta cggaggttct gcctaatcgt aatcgcccaa tgactgcgct gccgacgctt
    18961 attcgccata cgcccgggca aaaggaaccg gtactgcatt tttcacatgt cgcgccaatc
    19021 gattgtttaa ttcaacaatt acaacagcgc ctgcgtgatt gcgatattaa gcatctgacc
    19081 gatgtgctgg aaatagataa agatatgcgt gcgcttatgc agcggatcga aaatggcgtc
    19141 gctttcgaaa aatattatct gggtgcccag cctatttttc attgtctgaa aagtttgaat
    19201 gatttagtgc gactggcgaa ggcgctggac gtcgattttc ctttttctgc ttacgccgcc
    19261 attgagcata ttcccgtgat tgaagtggag tttgtccatc tggcggggct ggaaagttat
    19321 cccggtcagt tgacgttact ggataccccc gggccaaatg aagccgggca accgcatctg
    19381 caaaaaatgc ttaaccagca gctggcacgc gcctcggcgg tactggcggt gctggattat
    19441 acgcaactga aatcgatctc cgatgaagag gtccgtgagg cgattttggc ggtggggcaa
    19501 tcggtgccgc tgtatgtgct ggtcaataag ttcgatcaac aggatcgtaa cagtgacgac
    19561 gccgaccagg tgcgggcact gatttccggg acgctgatga aaggctgtat tacgccacag
    19621 cagatattcc cggtgtcgtc gatgtggggc tacctggcga atcgggcgcg ctatgagtta
    19681 gccaacaacg gtaagttacc agcgccagag caacaacgct gggtggaaga ttttgcccat
    19741 gccgcgctcg gcaggcgctg gcgtcatgcc gatctggcgg acctcgaaca tattcgtcat
    19801 gctgccgatc agttgtggga agattcgctg ttcgcccagc caattcaggc gttgcttcat
    19861 gccgcttacg ctaacgcctc attgtatgct ctgcgatctg ctgcgcataa actgttgaat
    19921 tacgcgcagc aggcgcggga atacctggat tttcgtgcgc acgggttaaa cgtcgcttgt
    19981 gaacaattgc ggcaaaatat ccatcaggtc gaagaaagtt tgcagctatt gcaactcaat
    20041 caggcgcagg tgagcggcga gattaaacat gaaatcgagc tggccctgac ctccgccaac
    20101 cactttctgc gtcaacagca agatgcggtg aatgcgcagt tagccgcctt gtttcaggat
    20161 gattcggagc cgttaagcga gattcgtacc cgctgtgaga cactgttaca gacggcgcag
    20221 aacaccatca gtcgcgactt tacgctgcgt tttgccgagc tggaatccac cctttgccgg
    20281 gtgttaaccg atgttattcg ccccattgag caacaagtca aaatggaatt gagcgagtca
    20341 gggtttcgtc ctgggtttca ttttcctgtt tttcacggcg tagttcccca cttcaacact
    20401 cgccagctgt tcagtgaagt catttcgcgc caggaagcaa cggacgagca gagcacgcgt
    20461 ttaggcgttg tgcgtgagac tttttcgcgc tggttgaatc agcccgactg gggacgggga
    20521 aatgagaaat ccccgacaga aacggttgat tacagtgtgt tgcaacgagc attaagcgca
    20581 gaagtcgatc tctattgcca acaaatggct aaagttctgg cagagcaggt cgatgaatct
    20641 gttacggcag gcatgaatac ttttttcgct gagttcgctt catgtttgac agaattacag
    20701 acgcgtttac gcgaaagtct ggctctgcgt caacaaaatg aatcggtggt caggctgatg
    20761 cagcagcaat tgcagcagac tgtgatgact cacagctgga tttacaccga cgctcagctg
    20821 ttacgcgatg atattcaaac acttttcacg gcagaacgat attgaccaag acgttactgg
    20881 acggccccgg tcgcgtgctg gagtcggttc atccgcgctt tttagtggat ctggcgcagg
    20941 gcgatgatgc ccgccatcct caagcccatc agcagcagtt tcgtgaacga ctgatgcagg
    21001 aacttctttc gcgtgtgcag cttcagacat ggacgaacgg cggcatgtta aatgcgccgc
    21061 ttagcctgcg actgacaatg gtggaaaaac tggcgtcgat gctggatccc ggtcatctgg
    21121 cgctgacgca gatcgcgcag catctggcgc tgctgcaaaa aatggatcac cgccagcact
    21181 ctgctttccc ggagctcccc cagcaaattg ccgccttgta tgagtggttt tcagcccgtt
    21241 gtcgctggaa ggaaaaggcg ttaacgcaac gaggcctact ggtgcaggca ggtgatcaga
    21301 gcgagcaaat ttttacccgc tggcgtgctg gggcgtataa cgcctggtcg ttgcctgggc
    21361 gctgttttat cgttctggag gagttgcgct ggggtgcatt tggcgatgcc tgccgtctgg
    21421 gaagcccgca agcggtggtg ttgttgctgg gtgatttgcg cgagaaagcg acacaacatc
    21481 tggcagagag tatcaatgcg gcaccgacca cgcgtcacta ttaccatcag tggtttgcct
    21541 cttcgaccgt tccgacgggc ggggatcatg ctgatttttt aagttggctg ggaaagtgga
    21601 ccacggcaga taaacaaccc gtttgctggt cagtgaccca acgctggcaa actgtcgcgc
    21661 tggggatgcc acgactctgt tcagcgcagc gtttggtggg ggcaatggtc gaggaaatct
    21721 tctctgtaaa tttggcgtaa ataatcagtt acatcaatga gtcctaaacg aaatccatgt
    21781 gtgaagttga tcacaaattt aaacactggt agggtaaaaa ggtcattaac tgcccaattc
    21841 aggcgtcaac tggtttgatt gtacattcct taaccggagg gtgtaagcaa acccgctacg
    21901 cttgttacag agattgcatc ctgcaattcc cgctcccctt ttgcggccgt cgcgctgatt
    21961 tttctggcgt ttgcggaaat gggccaactc tgcgaggaaa gctatgctga aaaggaaaaa
    22021 agtaaaaccg attacccttc gtgatgtcac cattattgat gacggtaaac tgcgtaaagc
    22081 cattaccgca gcatcactgg gtaatgcaat ggaatggttt gattttggtg tttatggttt
    22141 tgttgcttac gcattaggta aagttttttt cccgggggct gaccccagcg tgcagatggt
    22201 tgctgcactt gccactttct ccgttccctt tctgattcga ccgcttggcg gactcttctt
    22261 tggtatgttg ggcgataaat atggtcgcca gaagatcctc gctatcacta ttgtgattat
    22321 gtcgatcagt acgttctgta ttggcttaat accgtcctac gacacgattg gtatttgggc
    22381 accgattctg ctgttgatct gtaagatggc acaaggtttc tcggtcggcg gtgaatatac
    22441 tggggcgtcg atatttgttg cggaatactc ccctgaccgt aaacgtggct ttatgggcag
    22501 ctggctggac ttcggttcta ttgccgggtt tgtgctgggt gcgggcgtgg tggtgttaat
    22561 ttcgaccatt gtcggcgaag cgaacttcct cgactggggc tggcgtattc cgttctttat
    22621 tgctctgccg ttagggatta tcgggcttta cctgcgccat gcgctggaag aaactccggc
    22681 gttccagcag catgttgata aactggaaca gggcgaccgc gaaggtttgc aggatggccc
    22741 gaaagtctcg tttaaagaga ttgccactaa atactggcgc agcctgttga catgtattgg
    22801 tctggtaatt gccaccaacg tgacttacta catgttgctg acctatatgc cgagttattt
    22861 gtcgcataac ctgcattact ccgaagacca cggggtgctg attattatcg ccattatgat
    22921 cggtatgctg tttgtccagc cggtgatggg cttgctaagt gaccgttttg gccgtcgtcc
    22981 gtttgtgcta cttggtagtg ttgcactgtt tgtgttggcg atcccggcgt ttattctgat
    23041 taacagtaac gtcatcggcc tgatttttgc cgggttactg atgctggcgg tgatccttaa
    23101 ctgctttatg ggcgttatgg cttctacctt gccagcgatg ttcccgacgc atatccgtta
    23161 cagcgcgctg gcggcggcat ttaatatttc ggtgctggtt gccggtctga cgccaacact
    23221 ggcggcctgg ctggtcgaaa gctcgcagaa tctgatgatg cctgcctatt acctgatggt
    23281 agtggcggtg attggtttaa tcaccggcgt aaccatgaaa gagacggcaa atcgtccgtt
    23341 gaaaggtgcg acaccggcgg cgtcagatat acaggaagcg aaggaaattc tcgtcgagca
    23401 ttacgataat atcgagcaga aaatcgataa tattgaccac gagattgccg atttgcaggc
    23461 gaaacgtacc cgcctggtgc agcaacatcc gcgaattgat gaataagctg aaacggatgg
    23521 cctgatgtga cgctgtctta tcaggccaat tgaactctta aggttcactt aatctctgac
    23581 gcgcatactc tcctccaggt taacggagga gagtgcaatg aaaaaccgtg tttatgaaag
    23641 tttaactacc gtgttcagcg tgctggtggt cagcagcttt ctttatatct ggtttgccac
    23701 gttctgatct ttcttcagcc gtacccaggc ccgcgtgccg gaagtctctt gccggttttg
    23761 caggaaaaac tgcccgtgat gcaactgtgt aatgcggctg acaatactta accccagacc
    23821 aatcccgcca taacggctgt ccatacgtac aaacgcttta ctcaactccc cgcatttact
    23881 ctcatcaata cctggtcctt catcttcaac tgccatgacc gctccgccat cttcttgcag
    23941 cttaatcata atgttgctgc cttgcgggct gtaacgatgg gcgttttcta ccaagtttcg
    24001 caataacatc cgcagcaggg ttgcatcacc ctgaacggtg atgtcggcgg cgctctctgg
    24061 caatagcagg gtttgctgtc gctggtcgag catggtactg agttcgtcat acgaggggag
    24121 aatgacatct tccagcagtt ttacatgttg ataattaccg gaagaaaatg actgtccggc
    24181 acgcgccagt tgcagcagct gggagacgct ctccatcatc tgatcaagcc gtgccactaa
    24241 cggtgctaca tcaatgtgat gcgttttcgc cagcagttcc agatgcaaac gcacccccgc
    24301 cagtggcgtt cgcagttcgt gcgcgacgtc agcggtaaac aacctttcgt tatccagcgt
    24361 gctggtcagg cgactgacca gatcgttcaa cgccgaaacc accgcttcga tttcgagggt
    24421 ggcgctgtga atggcaatgg gcgttaagtt gtcggcggtg cgcgcttcca gctctttttg
    24481 cagctccgcc agcgggcggg tgatgcggcg taccgcctga tagcagataa atagcgtcag
    24541 gctgaccata aagacgccgg ggacaatcag gctggcgacc gcctcgcgga tctcacgcat
    24601 gatgtggcga tcgttgttgc gattgtcgcg tagcgcctgc tcaaacagct gaatctgctc
    24661 ggtactttca tgccatagcc agaagacgct gatcagctca aacaccaaca aaatggcccc
    24721 gatggtcaat atcagccgtt ggcgcagcga tattggtcgg cgcagaaaac gcatcagatt
    24781 caattagttt tcctcattcg cgaccagcat atagccaaag ccgcgcatgg tgcggatacg
    24841 ggctttgccc actttgtcgc gcagattgtg gatatgcact tccagggtgt tggtcgaggg
    24901 ttcattgtcc cagttataga tgtcgttgta gagaatttcc cgatgtaccg gactgcctgc
    24961 tttgagcatt aaccgtgaca gcagagcata ttctttgggc gtcagaatca actcttcacc
    25021 gcccatccat acctgacggc gacccatatt cagcgtcaga ttgccaacaa tcagctcact
    25081 ttcgccctga ttattatggc gtcgtagcag ggcgcggata cgggcgtgta actcttccag
    25141 cgcaaaaggc ttcaccagat agtcatcggc accgacatcc agcccggcga ttttgtcggt
    25201 cagcgtatcg cgagcggtga ggatcagtac cggcagggtg tattttttct gccggatacg
    25261 ggcgagaaaa tgcagtccat cttcgtcggg taaccctaaa tccagtacca ccaggctgta
    25321 atgaccgtcc tcaaggcttt gttccgccat ccgcgcggtt gtcacgccat cgcacgcgta
    25381 gccttcggtt tgcgccgcca gaatcagtcc ctgcaataac agcgtatcgt cttcaacaat
    25441 cagaattttc atttactcac tctcctgcaa gtttgcagaa tatcatccgc ggcctggtaa
    25501 tacttcgtct caacgccagt taatcccaat agcgtggaga ataaattgtc ttgtgaatag
    25561 tgttgcgttt gcgcctgttt ttgcaggcag ttctggtcaa cctgataccg cttttgataa
    25621 tcctccgaca gccacagcag cattggcacc tgtttttggc tatccggggc gatggcataa
    25681 ggcagaccgt gcagatagat gccattttca cctaacgatt caccgtggtc agaaagataa
    25741 accaggctgg tggtaaattt atcctgatgt tctttaagca gattaatcgc tttatcaaca
    25801 atatagtcga cgtaaaccag cgtgttgtcg taagtgttca ccagttgctc tttggtacag
    25861 gtctggatct cattggtgtc gcaggttggg gtaaatttcc tgaactgcgg cggatagcgg
    25921 ttgtaatagg tcggaccgtg gctgccgatg gtgtgtaaga caatcacgcc atcaccctgc
    25981 aggttattga tgtactcttc cagcccgtgg aacagcactt cgtcatagca ttcgccgttg
    26041 atgcactgat caggcagatt cagcgcggtg acgttctggt gaggcacgcg gtcgcaggca
    26101 cctttacagc cgccatcgtt gtcattccac agcacgttga tgcccgctcg ctgaatgata
    26161 tccagcacgc cttcctggtg ctgtgccagc tcttctttgt agtgctcacg cggcatatcc
    26221 gagaacatgc acggtactga aactgccgtt gccgtgccgc aagatgcggt attagggaaa
    26281 tagaccacgt tatctttcgc cagccgcggg ttagtttcac gcgggtagcc gttgagggag
    26341 aagttctccg cccgcgaggt ttcgccgaca atcaggatgg tcaaattttt acgtttttcg
    26401 ttctgcatta acgggttgcg gtgcgcgtct tcaccaattc gcaccagcgg cagatttgcc
    26461 agtcgctgat gggagtacca tgaccagctg gcaacaatgc tgttagaggg gcttaaggat
    26521 ttcaccagct ctttgttatt gcggaacaac gaggcgtagt ctttataaaa cagtgcggcg
    26581 accagcaaaa tcagcagtac agaaaccaga atattggctc cacggaaaag aacactgcgc
    26641 agacgcgagg tggcaggttt gatttttagc cagcaggcaa tcagcgcagc aagcacgccg
    26701 ctgaatccca gcgttaataa catttgcggt gtcatcagcg cataactttc tgctggagtg
    26761 gtatcaataa tattggcaat catcgagcgg tcgatgacga tgccgtaagt cattatgaaa
    26821 tattgtgcag ccgcgccaac cagaataaac aggcaggcca gtggtcgatt aagccataag
    26881 aaagagctta gtgtcaggac aatattaatc acgctgaaag cgacgaccgg catcgacaag
    26941 aaaaccagta cgttatgcag cgaatccagc ggcagcgcct gcaacacctg tttaaaaaag
    27001 gcaatattca ggcggataga gatataaaaa gcggccaaca atagccaggc gagtaaattc
    27061 aaagagggtc tttttagtag gcgcttcaac atcacggtgt ttccatcgaa caaagtgcgc
    27121 atatgctcgc aaagcaaaat taagccaacc ttaagttctt aaggttggct tttatgtttg
    27181 ctggattaag gggaattaat ctttgcttat tggtgcatct aagggatacg ggtttttatg
    27241 tagccggttg taattcagcg catacatggc ggtgatgacc atcagggtga caaatgacca
    27301 cataacctct ttcgccccgg accccaccac ggcccaaatg cagtagagga aggcaatggt
    27361 agtaacagcc agatatgccg ggcgtgcttt accaaagtga ccgtggccga gcagcagtaa
    27421 cgccgcacag gtgtaaagat atggcaccag tgtaaagatg accgagacgg aagaaaccag
    27481 accgaactct ttggtcgcgt ttggtgaaat gctgctgagc tggaagatgg tcatcaaaat
    27541 accgacgata atcagccccg ccactggcgt acccgcttta tttacacggg caaaaatcgg
    27601 tgggaacagt ctgtcatcgg cagcggcttt cgccgtttga cccgccagca acgtccagcc
    27661 gcccagtgaa cctaagcaac ccgcagctgc gcagaaggaa acaatggccc cagcggtgtc
    27721 acccaacgcc atccgtgcgg catcaccgaa tggcgaggca gaaacgcgca gtgcggcatt
    27781 agggatcatc cccataatcg cggtggtgga gagcacatag caaacggcgg caatcaatac
    27841 cccaccaatg gtggcgatag ggacattgcg tttcgggttt ttcaccacac ctgcggcaac
    27901 ggaggcactt tccacaccga tgaacgacca cagcgtaacg ttaagggtac tttgaatcgc
    27961 accgaaggtg cccaggccgc tgacgttcca tgccgccata taggtttcac cacggaacca
    28021 gaaccagcca aatacggcaa tcccgacgat ggggatcagc gccagcacag tggcaacggc
    28081 ctgcacgcgg gtgatcattt tcggaccgac aatgttcagc aggacgaaga tccacagcac
    28141 cacgacgcag gtgatggtta ataccaatgg atctttcaga atcgggaaga agtaacttaa
    28201 atatcctacg ccaatgacca ccatggcgat attgccgatc cagcaggcca gccagtagag
    28261 gacgttggtt tgataaccga gaaacgggcc aaagcagcgg cgggcgtaag cgtaagaacc
    28321 accaggactt gggtcgagga acgacatttt ggcgtatacc atcgagagcc ccagcgcacc
    28381 gataatcgtc accaaccatc cataaatggc aatcccgcca gtggaggcca ggtttgcagg
    28441 taacagaaaa acacctgacc ccataatatt ccccgacacc atcagggtga cggggattaa
    28501 gcccactttg tgagcatcag catccgaaga cataattaaa ctcctgcgaa ggcgagcttc
    28561 gtgacaataa attacgtcat atcatacgcc tgcattcgat agttgaatta ttatcgaccg
    28621 ggttatcgct gatgccgtta ttaatatagc tcttcgttaa ggttaaaaat caggcgacag
    28681 tgcgttctct gtgctgacta acataatgca gcggcgtcat accgtagaag tctttaaaga
    28741 cagaaataaa gtacgacgta ctgttgtagc cgcaggactg tgatacctga gagatatttt
    28801 taccgtccat cattaattca tttacggcat aacgcatgcg gcaggtggtg attatttggc
    28861 tataactggt gttttcgctt ttcaactttt tctttaacag acttgggcta agacataaac
    28921 aactggctac catactaaga ttccagtctt tgtgaatatc gctttcaata atttgataaa
    28981 cgctgtcact tacacaatta cgtaacatat acatcattaa tgaaaggaat tttttactgt
    29041 cgagaaaacg ggatagcacc gtgaataata atgcgcgtgt tcgttccttt tctgactcag
    29101 tttctgcggg catcatgctg tgttgcgcgg caacgcgaaa gacgtctggc gtcaggcatg
    29161 gcagggtgag tatgggcgta gcgctacgtt gccatactgg tatttgcgag agatctttat
    29221 tcagaaaccg gaggtaatct ttgatgatgt cgtgactaat atgagcaacc aaagtgttgt
    29281 taagagagga gacgtcgata atattatttt cacaatttaa taacgccata tggttagctt
    29341 taaggctaat aggttctttc ccattcaccc ttatccagac atctttttca gtcaataaaa
    29401 caatacaagg ttggtcgctg caaatcctca tatatgtact cctgagtgcg aataaaagac
    29461 cagttctcgc taaagcaaag cgataccggg aaatggttat ataattgtat gatgaatata
    29521 aacatgcata aaaataacga gttttactaa tattacaaat ttttatagta atttctcatg
    29581 tacatatttt tttacaggat aaataacagg caaaaaaaag cgcggggtgg ggccgcgcaa
    29641 aaagtatgac atttttgttt gcagcaattt ttatgaaaac gttgccatac tcaaaaggcc
    29701 taaggccagg cggcgtaatg ttatttaaac aattacgcct tcagcggaat agtggttacg
    29761 ctttcacgca cataacgtgg taaataccgt caataatttc agtcccttca gtttcgtgtt
    29821 caaatccagg gaaatggtgg tcccaggatt gcagcgagcg taaataactt acttgcggac
    29881 tgtttttatc gccgaagttt tcaccagaca gcagcatcgg gattcctggc ggatacggga
    29941 taactgagtt tgccgcgatg cgtcctggca gattttcaat ggataccagt tcgacattgt
    30001 tgtcgacaat cgcgttgtac gcttcacgcg gggtgatttc cgccaccggc aggccggaat
    30061 aggcttcgtt caaccgtgcg ccagggttgt tttctttcag ccaggcaaac atggtgtcac
    30121 ccagatcgtg aatccccatg ttcgcgtaag tgtcaggata ttgttcaaca agttccggca
    30181 tcacctgcgc cagcggtgtg ttggcgtcat agtggcgttt gaaggagcaa agggtgttaa
    30241 ccagagtccc ccatttccca cgggttacgc ccatagagaa caggaacata atttggaagt
    30301 cagtggtgcg ggtaggcaca atgccgtggc gaccaagcca ggcagtgacc agcgccgccg
    30361 gaacaccggt ttcttccagt tcaccatctt cacccattcc cggagcaagg atgctgactt
    30421 taattgggtc gagcatactc cagttatccg gaatatcttt gaagccgtgc cagctttcgc
    30481 ccggatgcat tacccagcag tcctgaacgg tggtcagcag tttggttggt gcgtcagcaa
    30541 agtcataggt tttgccggtt tgtgggtcgg tgacgacttc tttgttccac ggtttgaaga
    30601 accagctacc gtcagcggtg aactctttat atagccgcgc catcgcctga cggaaatcaa
    30661 ccgcgtcgtc aatcacttcc tgtgtcagtg acaggccgct gttgccgtcc atcatcgaca
    30721 ccgccacgtc gttggatgcg cagatggcat acagcgggga ggtggtggca tgcatcatgt
    30781 aggcctggtt gaagcgggag aagttaatcg ccccacgacc ttcacgtaca tgaatataag
    30841 aagcctgtga cagcgcattc agcagtttgt ggatggagtg ggtggcgaaa acggtaggac
    30901 cgttgtgatc gccaggttcg ccgcgcatgg catagtgatc ggcatagatc gggttgaaac
    30961 gtgcatagcc gtaccaggct tcgtcaaagt gcagacgatc ggaggttttt tccagcagat
    31021 cctgcgcttc tttagcgtta taacacacgc cgtcataggt gcagttggtt accacgcagt
    31081 aagacggttt ttgcccggct ttgtctttgg tcagcgggct ttcactgatt ttcttttgca
    31141 aggtttcagg ttgcatttcc tgcggataga ttggcccgat aatgccgtag cggttgcggc
    31201 ttggcaccat atagaccggt ttcgcgcctg tcagcatcaa accttgttcg atggatttat
    31261 ggcagttacg gtcaacgacc acgacatcgt tatcggtcat gcaagcctgc atgatggtgc
    31321 ggttagagcc ggaagtaccg acgactaccg accaggagcg atcggcacca aatacgcgtg
    31381 cggcatattt ttcgctttcg ccaaatgcgc cagtatggtc aagcaaagaa ccgagggaag
    31441 ttcgttcgat gcccatgtcg gtgcggaaca gattttcacc atagtagtca tggtagaaac
    31501 gtccggcggg tgttttggta aaaccaacgc cgccctggtg gcctggcgct gcccaggaat
    31561 attcatggat gtcactatat ttcatcagcg cgctgaacag tggcggcaac agctgctggc
    31621 ggtagcgggt catcgcggca acggcgcgtc cggcgataaa gtcggcggta tcttccagaa
    31681 tccaggcgaa ttcatcgaca agctccagca gatcccggtc cattgcggcg agggcttttt
    31741 cccgatcgcc caacaggaag accggcacgt tttgttggcg ctcatgaagc ttaccgatca
    31801 attgtctgac gttttgatgt tcgtccggat gttccatttg atagctgaac atcaggcagt
    31861 caatggcttc gtttgaagag agaatggcaa aaccatcatc aaaggaggtg gatttaatca
    31921 cggtaacatt ttgctggctt aaagcatctg ccagacgctc aacggcgtta ccgacccagg
    31981 tgtcttgatg gagaaactcg ctttcaacaa ttaatacttt catcattgct tacccggtta
    32041 tgaaggaatc ttcgttgcgg gcaagtatct tccggccttt tcgggctgta aagcgcgtga
    32101 aaaacaaaca tttttgaaat atttagtttt ataaacaata cgttatgtga ttattttaaa
    32161 ttattttcac tgcttattat cgacggctaa actatttttt tggctgatgc tgatatcgtc
    32221 tttagccggg aaacgtctgg cggcgctgtt ggctaagttt gcggtattgt tgcggcgaca
    32281 tgccgacata tttgccgaac gtgctgtaaa aacgactact tgaacgaaag cctgccgtca
    32341 gggcaatatc gagaatactt ttatcggtat cgctcagtaa cgcgcgaacg tggttgatgc
    32401 gcatcgcggt aatgtactgt ttcatcgtca attgcatgac ccgctggaat atccccattg
    32461 catagttggc gttaagtttg acgtgctcag ccacatcgtt gatggtcagc gcctgatcat
    32521 agttttcggc aataaagccc agcatctggc taacataaaa ttgcgcatgg cgcgagacgc
    32581 tgtttttgtg tgtgcgcgag gttttattga ccagaatcgg ttcccagcta gagaggctaa
    32641 atcgcttgag catcaggcca atttcatcaa tggcgagctg gcgaatttgc tcattcggac
    32701 tgtttaattc ctgctgccag cggcgtactt caaacgggct aagttgctgt gtggcaagtg
    32761 atttgatcac catgccgtga gtgacgtggt taatcaggtc tttatccagc ggccaggaga
    32821 gaaacagatg catcggcaga ttaaaaatcg ccatgctctg acaggttccg gtatctgtta
    32881 gttggtgcgg tgtacaggcc cagaacagcg tgatatgacc ctgattgata ttcacttttt
    32941 cattgttgat caggtattcc acatcgccat cgaaaggcac attcacttcg acctgaccat
    33001 gccagtggct ggtgggcatg atatgcggtg cgcgaaactc aatctccatc cgctggtatt
    33061 ccgaatacag cgacagcggg ctgcgggtct gtttttcgtc gctgctgcac ataaacgtat
    33121 ctgtattcat ggatggctct ctttcctaga atatcagaat tatggcagga gtgagggagg
    33181 atgactgcga gtgggatcac ggttttcacc ctcttcccag aggggcgagg ggactgtccg
    33241 agcatcatga ggccgaaaac tctacttttc aggtaattta ttcccataaa ctcagattta
    33301 ctgctgcttc acgcaggatc tgagtttatg ggaatgctca acctggaagc cggaggtttt
    33361 ctgcagattc gcctgccatg atgaagttat tcaagcaagc caggagatct gcatgatgtc
    33421 tgcacccaaa attacattta tcggcgctgg ttcgacgatt ttcgttaaaa atattcttgg
    33481 tgatgtgttc catcgcgagg cgctgaaaac ggcgcatatt gccctgatgg acattgaccc
    33541 cacccgcctg gaagagtcgc atattgtggt gcgtaagctg atggattcag caggggccag
    33601 cggcaaaatc acctgccaca cccaacagaa agaagcctta gaggatgccg attttgtcgt
    33661 ggtggcattt cagattggcg gttatgaacc ttgcacggtg actgatttcg aggtctgtaa
    33721 gcggcatggt ctggaacaaa ccattgccga tacgttgggg ccgggcggta ttatgcgcgc
    33781 gctacgtacc attccgcatc tgtggcaaat ttgcgagaac atgacggaag tctgccccga
    33841 tgccaccatg ctcaactatg ttaacccaat ggcgatgaat acctgggcga tgtatgcccg
    33901 ctatccgtat atcaaacagg tcgggctgtg ccattcggtg cagggaacgg cggaagagct
    33961 ggcgcgtgac ctcaatatcg acccggctac gctgcgttac cgttgcgcag gtatcaacca
    34021 tatggcgttt tacctggagc tggagcgcaa aaccgccgac ggcagttacg tgaatctcta
    34081 cccggaactg ctggcggctt atgaagcagg gcaggcaccg aagccaaata ttcatggtaa
    34141 tactcgctgc cagaatattg tgcgctacga aatgttcaaa aagctgggct acttcgtcac
    34201 ggaatcgtca gaacattttg ccgaatatac gccgtggttt attaagctag gtcgtgagga
    34261 tttgattgag cgttataaag taccgctgga tgagtacccg aaacgctgcg tcgagcagct
    34321 ggcgaactgg cataaagagc tggaggagta taaaaacgcc tcccggattg atattaaacc
    34381 gtcacgggaa tatgccagca caatcatgaa cgctatctgg actggcgagc cgagtgtgat
    34441 ttacggcaac gtccgtaacg atggtttgat tgataacctg ccacaaggat gttgcgtgga
    34501 agtagcctgt ctggttgatg ctaatggcat tcagccgacc aaagtcggta cgctaccttc
    34561 gcatctggcc gccctgatgc aaaccaacat caacgtacag acgctgctga ccgaagctat
    34621 tcttacggaa aatcgcgacc gtgtttacca cgccgcgatg atggacccgc atactgccgc
    34681 cgtgctgggc attgacgaaa tatatgctct tgttgacgac ctgattgccg cccacggcga
    34741 ctggctgcca ggctggttgc accgttaaaa cgcgactaaa cgctactgcg ccgggggatt
    34801 tattccggcg cacacctctg acgataccaa taacagaagg cgggcgttgg taacagcgac
    34861 ccgataccct atgagcattt caatgactac aaaactcagt tatggatttg gagcgttcgg
    34921 gaaggatttt gcgatcggca ttgtgtatat gtacctcatg tattactaca ccgatgtcgt
    34981 cgggctgtct gtgggtttgg tcggtacttt gtttctggtg gcgaggatct gggatgctat
    35041 taacgatccg attatgggat ggattgtaaa tgctacgcga tcgcgatggg gtaagttcaa
    35101 actctggatc ctgatcggta cgttggcaaa ctctgtaatc ttatttctcc tctttagtgc
    35161 gcatctgttt gaaggtacta ctcagattgt ctttgtttgc gtgacctaca tcctctgggg
    35221 catgacttac accattatgg atattccctt ctggtcgctg gttccaacca tcacgctcga
    35281 taaacgtgaa cgcgaacaac tggttcctta tccgcgtttt tttgccagtc tggcaggctt
    35341 tgttacggca ggtgtgacgc taccatttgt taattatgtc ggcggtggcg atcggggatt
    35401 tggctttcag atgttcactc tggtattgat cgcctttttt attgtttcaa ccatcatcac
    35461 tctgcgcaat gtgcatgaag tcttttcgtc agacaatcaa tcgtctgctg aaggaagcca
    35521 tctgacactt aaagccatcg ttgcgcttat ttataaaaac gatcagcttt catgcctctt
    35581 gggtatggct cttgcttata atgtagccag caacattatt accggatttg ctatctatta
    35641 tttctcatat gttatcggtg atgcggattt gttcccctat tatctgtcgt atgcgggagc
    35701 tgctaacctg gtgacgttag tattcttccc acgcttagtt aaatcattat cccgacgcat
    35761 tttatgggcc ggagcatcta ttcttccggt gttaagctgt ggtgttctcc tgttaatggc
    35821 attaatgagc tatcacaacg tcgtcctcat tgtgattgcg ggtattttgc tgaatgtggg
    35881 aacggcgctt ttctgggtat tacaggtcat catggtggca gataccgttg attacggtga
    35941 atataaactg cacgtacgct gtgaaagcat cgcttactcc gtgcagacta tggtggtgaa
    36001 gggcggttca gcctttgcgg cttttttcat tgcggttgtg ttagggatga ttggctatgt
    36061 accgaatgtt gaacagtcta cgcaagccct attaggtatg cagtttatta tgattgctct
    36121 accgactctg tttttcatgg taacgctgat tctctacttc cgtttctatc gcctcaatgg
    36181 cgacacgctg cgcaggatcc agattcatct gctggataaa tatcgcaaag taccgcccga
    36241 gcctgttcat gctgatattc cggtcggtgc agtgagtgat gtgaaagcct gacgtggaca
    36301 atagctaacg aaaatggcct gatgcgatat gtttatcagg ccacgtctta atgtaatgct
    36361 ttggatttgc aaattttgta gaccggataa ggaattcacg ccgcatccgg catcaacaaa
    36421 gtgcacgttg ttatccggtt atcaagccat agcgccgtag ctggcggcaa tgaaagcgag
    36481 cgagcaatat cactgtcgtt aatgcgccaa gcagcgcgca gaacatatcg gattgcgtgt
    36541 cccactggtc gccctgggta ccgagaaaat catccgctcc ctggcccatc gccagtgccg
    36601 cccaccactc gattaattca tacatcgcgc ttatcgccag cgccacgcaa cagaccagaa
    36661 acgccaccat tttacgtccg cgcacgtaca tcccgcgaac gagaatttct cgtgccacca
    36721 gtgcaggcac cagcccctgg aaaaagtgcc ccagcttgtc atacggatta cgactcaacc
    36781 ccagccattc ctgcacctca aaaccaacgg ggactttcgc gtaggtgtac tgcccgccta
    36841 ccatcaggat gatggcgtga aagaagatga gcgtatagag cagcagcgtt aacgggtaac
    36901 gtctggcggt ggcaagtagt agcggcacga cgataatcac cggtgtcact tccagcagcc
    36961 aggtgagttt gtcatgggcc gaaatgccgg tataaatcag gattaacgtt agcgccagtg
    37021 cgccggtgtt aagaattaac ggcttgagtg tgcgggtcat ggtgagttcg caagtcaggg
    37081 aaaatactga ctattcacca gcaagcatga aattacaatc gcaaaagagg tggcccaggg
    37141 ggatcacctg gcagcatgct gccaggcgct gggccgaaga ggttacttag tgcagttcgc
    37201 gcactgtttg ttgacgattt gctggaagaa gtcgttacct ttgtcatcga ccaggataaa
    37261 cgccgggaaa tcttctactt cgattttcca gatagcttcc atacccagct ccggataagc
    37321 gacgcattcc agatgcttga tgctctgctg cgccagtacc gccgccggac cgccgatgct
    37381 accgaggtag aagccgccgt gtttatgaca cgcgtcggta acctgctggc tgcggttgcc
    37441 tttcgccagc atgatcatgc tgccgccgtg ggattgcagc agatccacat aggagtccat
    37501 acgacctgcg gtggttgggc caagtgaacc tgatggataa ccggcagggg ttttcgccgg
    37561 acccgcgtag tagatcgggt gatctttgat gtactgcgga agttctttac cggcgtcaat
    37621 cagctctttc agcttggcgt gtgcaatatc tcgacccaca ataatggtgc cagtgagcga
    37681 caaacgagtg gaaaccggat attgcgaaag ctgggcgagg atctctttca tcgggcggtt
    37741 aaggtcgacc ttcaccgctt cgccttcacc ggcctgacgc agttcttgtg gaatgtactg
    37801 acctagattg tgttccagtt tttcgatcca gataccttcg cggttgattt tcgctttaat
    37861 gttacggtca gcggagcagg agacgcccat gcctaccggg caggatgcgc cgtgacgtgg
    37921 cagacggata acgcgaatgt cgtgcgcgaa gtatttaccg ccaaactgcg cgccaagacc
    37981 gagtttctgg gcctcttcca gcagttcctg ttccagctgg acatcgcgga acgcctgacc
    38041 atgttcgttc ccttccgtcg gcagttcatc gtaatagtga gcgcttgcta atttgacggt
    38101 tttcaggttg gtttccgcag acgtaccgcc aatcacaaac gcgatatggt acggcgggca
    38161 ggctgcagta ccgagggtac gcattttctc gacgaggaag tttttcagtt tgccgggagt
    38221 cagcagggct ttggtttcct ggtagagata cgttttgttg gcagagccgc cgcctttcgc
    38281 aacgcaaagg aatttgtact catcgccatc taccgcgtac aggtcgattt gcgcaggcag
    38341 gttagtgccg gtgttgacct ctttgtacat gtccagcgcc gcgttttgcg agtagcgcag
    38401 gttatcttcg atataggtgt tatagacgcc tttcgacagc gcttcttcat caccgccacc
    38461 ggtccacacg cgctggcctt ttttaccgac gatgatcgcg gtgccggtat cctggcaggt
    38521 cggcagcacg cctttggcgg cgatttcgga gtttcttaag aattgcagtg ccacgtactt
    38581 gtcgttttcg ctggcttctg gatcgtgaag aatagccgca acctgtttct ggtgtgccgg
    38641 acggagcatg aaagaagcgt cgtgaaaggc ttgctgcgcc agcagggtca gggcttctgg
    38701 ttccactttc aggatggttt cgccgtcgaa gtcggcaacg ctaacgtaat cggaagtgag
    38761 tagatagtat tcggtattgt ctttccccat cgggaaaggt gcttggtaga taaagggttt
    38821 gtttgacata gcttccagcc tgtaactctg tatttgttat tcgaaaatgg cgtgccgcgt
    38881 agtcacgggc ggcacgcaaa gtgcatttat aagaacccgt acatcgcggc gaagatccag
    38941 ccgaagacgc acgatacgct cacaccaatc aaccccggca gaataaaact gtggttgatg
    39001 acgaagcgac cgatgtgggt ggtgccggaa cggtcaaact gaatcgctgc cagatcgctc
    39061 ggataagtcg gcaggatgta ataaccgtag caagccggtg ctgaagccac gatgtatgcc
    39121 ggatcaacgc cgatcgccag cgcgaccgga acaatcgccg ccagcgccgc agcctgagag
    39181 tttacaaact tggaaaccag cagcagaaca atggcatacg cccacggata ctctttcacc
    39241 atttcaccca gtacgccctg aatttcagac atatgcgcac cgaacatggt ttctgccatc
    39301 catgcgatac cgtacaccgc cacgatggcg atcataccgg aacggaagac ttcgtttttt
    39361 gagatagacg cgggattggt tttggtcagg ataataatca gcgccccggt cagcagcata
    39421 aacatctgaa taaccagtac catcgacagc ggtttgccgc cgaaggatgg acgcaggtcc
    39481 gaatcagcac caagaagggc gactacagcg attgccccga ggaaaatcca cattgccagc
    39541 cagttgcttt tcggcagttt tttatccagc agcgtcgcgg tatcaccgta aacatactca
    39601 cggttttccg gtacggagat gaatttctgg aactcttcgt ctttatccag atctttaccg
    39661 cggaaccagc tgaagatacc gatcgccagg ataccgatta acgtcgatgg aatggtgatt
    39721 gccagcagat cgaggaactc aagatggcga ccatcaaagg tgacattacc cagcatcgca
    39781 accagagaca cgaccgcaac cgacaccgga ctggcgataa tccccatctg tgcaccgata
    39841 gaacttgccg ccatcggacg ttccggacgg atgttgttct taatggcgac gtcgtagatg
    39901 atcggcagaa tggtgtaaac cacatgaccc gtaccgcaaa gaatggtcag tgtacaggtc
    39961 acaaacggcg cgacaattga gacatatttc gggttgcggc gcagcagctt ctcggcaatt
    40021 tgcagcatga catcaagacc gcccgaagct tgcaaggtcg ccgatgccgc caccaccgca
    40081 atgataacca gcatgacatc aactggtggt ttacctggct gaaggtggaa gacgaagacc
    40141 agaatgacca gaccgatacc gcctaataaa cccagcgcga taccaccctt tctggcacca
    40201 taaaacagac atatcagtat tatgataagt tggatagtaa ataacatgtg tgaaccctcg
    40261 cgataatcct atttaaattt ttgctgaata gatcacagtc acgttctgtt ttgtatgaac
    40321 tgttttcaga gcctgactaa atatccgttt ggtcgttacc ggctttagca aatacctcac
    40381 agtgaatatt ggctgattaa agcagcggca aatttatgca ctgtgataag cggctttttc
    40441 agagagaaaa actctctggc gatgggttta tagtttgtac tgcgcagatg ttaatgtttt
    40501 gtttgctgtt atcttattga tatttatcgg tctatttttt gttctcaggg cagatatcat
    40561 taggtttata gattaatctg atctacccat ttgtgggtaa aaatacacat aatgcgggtg
    40621 acataatagt taattaactt ttgttatcgt tttgaaatta aaaacaccgt tcatctgaag
    40681 agatattaat ttttaacgat gatggaggga taattatatt tgatctggca caagttttac
    40741 tgattaacga tgtaacttgt gccaggggta ttttgcatta cggtaataat tattacttac
    40801 agataaccag cgatccgtta ttggcaatat tgtttcagca gtgagtagtg ttctgcctga
    40861 atacggtaac ggtaaaccgg acgccccgtg acgccataat ggatactggt gaacaagatg
    40921 tggcagttga ccagccagat gaggtatttt cggcaggaaa cacgcgagat gttaacctcg
    40981 ttagccagct cgtcggttga aaactcatag tcctgatgcg cgtcaatcca ctggcacagc
    41041 gtgcgtaacg tctgcggcgt taagcctttt ggcaagcgac gaggatcctg ttcgttggag
    41101 ctgctgccgt ggattagctg atcaagctcg gcctggtcat aatactgatg tttttccagc
    41161 gccattttct tttgccgcca gccggtgagc gcctcttcaa agcgggaagc ctggaagggt
    41221 ttgatcaggt aatccacgac accgtaatgc agcgaatctt taatggttgc cgcatcggct
    41281 gcggaggaga tgacaatcac atcacttttg caacgcgcgt tatgcaggac aggcagtaaa
    41341 tcgagcccgt tctctttttg catatagata tcgagcaata tcaggtcgat aggcgcatcg
    41401 ctattgaaga taatctcttt ggctttctcc agcgtcgagg ctgttccaca gcattgaaag
    41461 cctgggattt gtgctacgta tcggcgattc agctccgcga ccattgcgtc gtcatcgata
    41521 attaatacat tgatcatctg ttcgacctct ccccgtccca gggtatctgg acaaaaaatt
    41581 gtgtgaaaat ccccggttcc gattccacgg cgatgctgcc gccgagattt tctacctgtt
    41641 gtttgacaag tgctaaaccg acgcctcgct cgcttccttt tgtcgagaca cctttgtcaa
    41701 aaatgtgatc gattttgtcg ggtgcgatcc ccggtccatc atcgttaact tcgcagtgca
    41761 gccagccgtg acggtagtgc aatgttacgc taatttcgcc accgggttcc ggccctaatg
    41821 cttccagcgc gttttctatc agatttccca atgtggtaat cagcgtcgcg acctggtcct
    41881 cactgccgct gtctggcagc tggctttcac tgtttaaaat cagcgtatgg cctaaatcgg
    41941 tcgcgcggtt aatcttgctg attaaaaaac cagcgataac cggagatttg atcttaccca
    42001 gcagagagcc aatctcttcc tgatagttat tggctgtttt gagaatgtaa tcttccaact
    42061 gcttataact cttcagatgc aataatccga gaatcacatg caatttattc ataaattcgt
    42121 gggatcgttc acgaagtgcg tcagcatagt tgaccagacc gtcgagtcgc tgcatcagtt
    42181 tacgaacttc agttttgtcc ctgaaggttg aaatggcacc gatgataacg ccattactgc
    42241 gcaccggaac ggtgttgatc agtaatagcc ggtctttaat cgtaatctct tcgtcgcggc
    42301 gcggcgtacc gtcgcgtaac acttccgaga catctaccac ctgtgaccat gagtggctga
    42361 gcgtcgacag tttctcatcg tcctgcgact tacggtaatt cagcaattct tgtgcggcat
    42421 cgttgatcag cgtgacctcg ccgcgatcgt ccacggcaac gacgccttct ttgatagact
    42481 gcaacatggc ctggcgttgc tcaaacagcg tggagatttc gtagggttcc aggccgaaaa
    42541 ggattttttt cagtacctta accagaatgc aggtgccaat cagtccgacc agcatgccaa
    42601 ataataccga ccagataatg ctccagcgac tgtcattgat ctgttgggtc acgcggctta
    42661 actcaaggcc gatcgccacc acgccaattt gtttatgatt ttcatcgtag atgggggtaa
    42721 atacgcgtaa agcctgcgcc agaaaaccgc gattgatagc gacattttct tcgccattca
    42781 gcgcattaag gatgtcatca cctttaaatg gctgaccaat acgctgggct tcaggatgtg
    42841 agtagcgaag actttgcata tcggtaacga caataaacag cagatcgttg cgtttgcgca
    42901 cggcttccgc gatggcctgg atgccactct cctgcggttt tttctgcaag ccctgacgga
    42961 tttccggcga gtcggcgagg gtacgcgcca ctgccagtgc cttgttggcc agcccgtctc
    43021 gcgtcatatc actgatttgc gagaagtaaa tcagatgcac caccaatagc accgagaaca
    43081 gtaccgcgct gaccattaag atcacagtgg tactcaattt catcggacgt ttgcgtaata
    43141 tgtggtaggg caatgaatgt ctcatcagct tccttgtgtg acaaatttct taagcattat
    43201 ctctgatgag gcgggtaatt caaagggagt aaaaatgatt ggctatatag gggaagagac
    43261 tctggcaacg gaaactgcca gtgctgtatg aagattccgg ggctatgctt atagcgataa
    43321 tcatactgat gagagaggga aggtcatgga tcaggcgcta ctggacgggg gttatcgctg
    43381 ttataccggc gaaaagatcg atgtctattt caacactgcg atatgtcagc attctggcaa
    43441 ttgcgtacgt ggcaacggca agttatttaa tctcaaacga aagtcg
//
LOCUS       DADLSA010000018        41241 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.018, whole genome shotgun sequence.
ACCESSION   DADLSA010000018 DADLSA010000000
VERSION     DADLSA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.018"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(173..1348)
                     /gene="ubiF"
                     /locus_tag="JN691_001006"
     CDS             complement(173..1348)
                     /gene="ubiF"
                     /locus_tag="JN691_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAY7242509.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVIPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1494..2918
                     /gene="miaB"
                     /locus_tag="JN691_001007"
     CDS             1494..2918
                     /gene="miaB"
                     /locus_tag="JN691_001007"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAY7242510.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3032..4111
                     /locus_tag="JN691_001008"
     CDS             3032..4111
                     /locus_tag="JN691_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAY7242511.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYQAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AWEEAEQKRKAALAAERKREEQEQK"
     gene            4108..4575
                     /gene="ybeY"
                     /locus_tag="JN691_001009"
     CDS             4108..4575
                     /gene="ybeY"
                     /locus_tag="JN691_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAY7242512.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4665..5543
                     /gene="corC"
                     /locus_tag="JN691_001010"
     CDS             4665..5543
                     /gene="corC"
                     /locus_tag="JN691_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAY7242513.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5568..7106
                     /gene="lnt"
                     /locus_tag="JN691_001011"
     CDS             5568..7106
                     /gene="lnt"
                     /locus_tag="JN691_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAY7242514.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7503..8411
                     /gene="gltI"
                     /locus_tag="JN691_001012"
     CDS             7503..8411
                     /gene="gltI"
                     /locus_tag="JN691_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HAY7242515.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8581..9321
                     /gene="gltJ"
                     /locus_tag="JN691_001013"
     CDS             8581..9321
                     /gene="gltJ"
                     /locus_tag="JN691_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HAY7242516.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            9321..9995
                     /gene="gltK"
                     /locus_tag="JN691_001014"
     CDS             9321..9995
                     /gene="gltK"
                     /locus_tag="JN691_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAY7242517.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            9995..10720
                     /gene="gltL"
                     /locus_tag="JN691_001015"
     CDS             9995..10720
                     /gene="gltL"
                     /locus_tag="JN691_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAY7242518.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            10838..11773
                     /gene="rihA"
                     /locus_tag="JN691_001016"
     CDS             10838..11773
                     /gene="rihA"
                     /locus_tag="JN691_001016"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAY7242519.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            11857..13527
                     /locus_tag="JN691_001017"
     CDS             11857..13527
                     /locus_tag="JN691_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HAY7242520.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(13587..15038)
                     /gene="djlC"
                     /locus_tag="JN691_001018"
     CDS             complement(13587..15038)
                     /gene="djlC"
                     /locus_tag="JN691_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HAY7242521.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(15035..15742)
                     /locus_tag="JN691_001019"
                     /pseudo
     CDS             complement(15035..15742)
                     /locus_tag="JN691_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            15844..16398
                     /locus_tag="JN691_001020"
     CDS             15844..16398
                     /locus_tag="JN691_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7242522.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16408..17835)
                     /locus_tag="JN691_001021"
     CDS             complement(16408..17835)
                     /locus_tag="JN691_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HAY7242523.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(17832..18539)
                     /locus_tag="JN691_001022"
     CDS             complement(17832..18539)
                     /locus_tag="JN691_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7242524.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHLSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            18703..19683
                     /locus_tag="JN691_001023"
     CDS             18703..19683
                     /locus_tag="JN691_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAY7242525.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWY
                     KQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAG
                     EGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECND
                     ATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(19753..20235)
                     /locus_tag="JN691_001024"
     CDS             complement(19753..20235)
                     /locus_tag="JN691_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAY7242526.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            20470..23052
                     /gene="leuS"
                     /locus_tag="JN691_001025"
     CDS             20470..23052
                     /gene="leuS"
                     /locus_tag="JN691_001025"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAY7242527.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23067..23648
                     /gene="lptE"
                     /locus_tag="JN691_001026"
     CDS             23067..23648
                     /gene="lptE"
                     /locus_tag="JN691_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAY7242528.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPINAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            23648..24679
                     /gene="holA"
                     /locus_tag="JN691_001027"
     CDS             23648..24679
                     /gene="holA"
                     /locus_tag="JN691_001027"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAY7242529.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            24681..25322
                     /gene="nadD"
                     /locus_tag="JN691_001028"
     CDS             24681..25322
                     /gene="nadD"
                     /locus_tag="JN691_001028"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAY7242530.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            25346..25957
                     /locus_tag="JN691_001029"
                     /pseudo
     CDS             25346..25957
                     /locus_tag="JN691_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            26217..26534
                     /gene="rsfS"
                     /locus_tag="JN691_001030"
                     /gene_synonym="rsfA"
     CDS             26217..26534
                     /gene="rsfS"
                     /locus_tag="JN691_001030"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAY7242531.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26538..27005
                     /gene="rlmH"
                     /locus_tag="JN691_001031"
     CDS             26538..27005
                     /gene="rlmH"
                     /locus_tag="JN691_001031"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAY7242532.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            27036..28937
                     /gene="mrdA"
                     /locus_tag="JN691_001032"
     CDS             27036..28937
                     /gene="mrdA"
                     /locus_tag="JN691_001032"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAY7242533.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKIDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKQWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            28940..30052
                     /gene="mrdB"
                     /locus_tag="JN691_001033"
                     /gene_synonym="rodA"
     CDS             28940..30052
                     /gene="mrdB"
                     /locus_tag="JN691_001033"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAY7242534.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30063..31151
                     /gene="rlpA"
                     /locus_tag="JN691_001034"
     CDS             30063..31151
                     /gene="rlpA"
                     /locus_tag="JN691_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAY7242535.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31290..32501
                     /gene="dacA"
                     /locus_tag="JN691_001035"
     CDS             31290..32501
                     /gene="dacA"
                     /locus_tag="JN691_001035"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAY7242536.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALDLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32612..32875
                     /locus_tag="JN691_001036"
     CDS             32612..32875
                     /locus_tag="JN691_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAY7242537.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            32976..33617
                     /gene="lipB"
                     /locus_tag="JN691_001037"
     CDS             32976..33617
                     /gene="lipB"
                     /locus_tag="JN691_001037"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAY7242538.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            33876..34829
                     /locus_tag="JN691_001038"
     CDS             33876..34829
                     /locus_tag="JN691_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAY7242539.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPNPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            35038..36003
                     /gene="lipA"
                     /locus_tag="JN691_001039"
     CDS             35038..36003
                     /gene="lipA"
                     /locus_tag="JN691_001039"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAY7242540.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36104..36307)
                     /gene="tatE"
                     /locus_tag="JN691_001040"
     CDS             complement(36104..36307)
                     /gene="tatE"
                     /locus_tag="JN691_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAY7242541.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36436..37224)
                     /locus_tag="JN691_001041"
     CDS             complement(36436..37224)
                     /locus_tag="JN691_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAY7242542.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            37317..37700
                     /gene="crcB"
                     /locus_tag="JN691_001042"
     CDS             37317..37700
                     /gene="crcB"
                     /locus_tag="JN691_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAY7242543.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(37754..37963)
                     /gene="cspE"
                     /locus_tag="JN691_001043"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(37754..37963)
                     /gene="cspE"
                     /locus_tag="JN691_001043"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY7242544.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(38138..38698)
                     /locus_tag="JN691_001044"
     CDS             complement(38138..38698)
                     /locus_tag="JN691_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HAY7242545.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            39285..40670
                     /gene="dcuC"
                     /locus_tag="JN691_001045"
     CDS             39285..40670
                     /gene="dcuC"
                     /locus_tag="JN691_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY7242546.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHNVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(40711..41052)
                     /locus_tag="JN691_001046"
                     /pseudo
     CDS             complement(40711..41052)
                     /locus_tag="JN691_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT        10345 a        10403 c        10281 g        10212 t
ORIGIN      
        1 aatcctcgta ccccagccac attaaaaaag ctcgcttcgg cgagcttttt gcttttctgc
       61 gttcattcaa tgtcgaatgc gatgttgaca cgtcttatcc ttcaatgtcg gatgcgacgc
      121 tgccgcgtct tatccgacct acggttggca cgcatccggc aatgttgtaa ggctacaacc
      181 ctaacgcata tttcagcgcc tgacgtttca acacgccagc acgctctgcc gccattaacc
      241 cgagattacg cacaaaacgc agcggcggca gattattact gaatccggca taaaacagat
      301 ccataccact ttgcataatg aagttatccg ccatgcgccg catctggtaa cgcttgagga
      361 caggataact ggcccacgct tcaccgtagc tgcgggcatt caccagaaca tcaatcaggg
      421 catcgacatc acgataacca agattcaccc cctgccccgc cagcggatgg atggtatgcg
      481 cggcatcgcc caccagcgca agccccggct gcacatactg caacgcatgt cggcgcgtca
      541 gcggaaacgc accagcggca agcggtataa cgtaacccag acgcgacggg aaatgcttcg
      601 cgatttccgc ctggagttgc gccatattca tattctgcaa ctggcgaata cgcgccggag
      661 agtcatacca caccagcgat gcccagttat caaacaacgg cagaaacgca cgcggtccgt
      721 ccggggtaaa ttgctgccag gtgctgtcgc cgggatcgtt ctcacactgt acgctaatca
      781 acatgcacga ctgcgcatac tgccaggcat gaacgccaat tcccgccatc tgccgcacct
      841 gagaatttgc gccgtcggca ccaatcacca gtttcgcgcg gatcgtttca ccgcccttca
      901 gttccagctc ctgaagatca ttatctcgat gtaatgcaat cagcgaggtt ggcacacgta
      961 acgttacttt cggatgcgct tccagcgcct gccacaacgc ctgttgcagg actgtgtttt
     1021 ccaccatgta gccaagcaac ggcaacttta attcggctgc gtcaaatacc acatgtgccg
     1081 tttcccactc ccaggtttcc agtctgcggt aaggatggca acgcatagcc tgtactgcat
     1141 cccagacccc taacccttta agcaatgata ccgaagccgc gctgatcgcc gagatccgca
     1201 cgtccggttg gctgtcagcg acaaacggtg caggttctgc gtgctcgatc accgttaccg
     1261 aaaatccgtg ctgtgccagc cccagcgcca gtgcgccgcc gaccattcct ccgccgacaa
     1321 tggcaatttc cgttggttga tttgtcatgg tcaatcatcc tgttagcaat atgctttaag
     1381 tttacaggat ttttaccccc aagggctgat aacgctcata ctggtcacaa cggcagcaaa
     1441 gcattacact atgcgccctg cattcctggc tactatttcg caagagcaag tcgatgacca
     1501 aaaaactcca tattaaaacc tggggctgtc agatgaacga gtacgattca tcgaagatgg
     1561 ccgatctgct ggatgccacc cacggctatc aactgaccga cgtggcggaa gaagcggatg
     1621 tgctactgct gaacacctgc tcaatccgcg agaaggctca ggaaaaagtc ttccatcagt
     1681 tgggtcgctg gaaactgtta aaagagaaga atccagacct gattatcggc gtcggtggct
     1741 gcgtggcatc gcaagaaggc gagcacattc gccagcgcgc ccactatgtc gatattattt
     1801 ttgggccgca aacgctgcac cgtctgccgg agatgatcaa ctccgtgcgc ggcgaccgca
     1861 gcccggttgt agatatcagc ttcccggaaa tcgagaagtt tgaccgtctg ccggaaccgc
     1921 gcgccgaagg gccgaccgcg tttgtctcca tcatggaagg ctgcaataaa tattgcacct
     1981 actgcgtggt gccttacacc cgtggtgaag aggtaagccg tccgtctgac gatattctgt
     2041 ttgagattgc ccagcttgcg gctcagggcg tgcgtgaagt caacctgctc ggtcagaatg
     2101 tgaatgcctg gcgtggtgag aactacgacg gcaccaccgg atcgtttgcc gatctgttgc
     2161 gtctggttgc tgcgatcgac gggatcgatc gtattcgctt taccaccagc catccgatcg
     2221 aattcaccga cgatatcatc gaagtgtacc gcgacacgcc tgagctggtg agcttcctgc
     2281 atctgccggt acagagcggt tccgatcgca ttctgaacct gatgggacgt acccatacgg
     2341 cgctggagta caaagcgatc atccgtaaac tgcgtgcggc gcgtccggat attcagatca
     2401 gttctgactt cattgttggc ttccctggcg aaaccaccga agacttcgag aaaacgatga
     2461 agctgattgc cgacgtcaat ttcgacatga gctacagctt tatcttctct gcacgtccag
     2521 gaacgcctgc cgccgatatg gttgatgatg ttccggaaga agagaagaag cagcgtctgt
     2581 atattctgca agaacgtatc aatcagcaag cgatggcatg gagccgtcgg atgctcggca
     2641 ccacccagcg tattctggta gaagggactt cgcgtaagag catcatggag ctttccgggc
     2701 gtaccgaaaa taaccgcgtg gtcaacttcg agggcacacc ggatatgatc ggtaaattcg
     2761 tcgatgtaga aattaccgac gtctacccga actctctgcg cggtaaagtg gtgcgaactg
     2821 aagatgaaat gggtctgcgc atggcagaaa caccggagtc ggtgattgcc cgcacccgca
     2881 aagaaaacga ccttggcgtg ggttattatc agccgtaatt ctcaggcctg ccgttccggt
     2941 gggccttgtt ttttctctct ttatccccat ctttatcgca atgcttgcgc ctttttcctg
     3001 tggcgtgaat aatttcctta taggccggtg aatgttcagc ttccggcggc atacagataa
     3061 gaggaacggt ttgaacatag acactcgcga aatcaccctg gagccagcag acaatgcgcg
     3121 tctgttgagc ctgtgcggcc cgtttgatga caacatcaag cagctcgaac gccgtctcgg
     3181 catcgagatc aatcgccgcg ataaccactt taaactgacc ggccgtccga tttgcgtcac
     3241 cgctgcggca gacattctgc gtagcctgta tgtcgatact gccccgatgc gcggtcagat
     3301 tcaggatatc gaaccggaac agatccacct tgcgattaaa gaagcgcggg tactggagca
     3361 aagcgcggag agcgtgccgg agtacggcaa agcggtcaat atcaaaacta aacgcggcgt
     3421 aattaagccg cgcacgccaa accaggcgca gtacattgcc aatattctcg accatgacat
     3481 taccttcggc gttggcccgg cgggtacggg taaaacctac caggcagtgg ctgcggcagt
     3541 tgatgccctg gagcgtcagg aaattcgccg tattctgctg actcgtccgg cggtcgaagc
     3601 cggtgagaaa ctgggcttcc tgcctggcga tttaagccag aaagtagacc cgtatctgcg
     3661 cccactgtac gacgcgctgt ttgaaatgct gggctttgag aaagtcgaga aactgattga
     3721 gcgcaacgtt attgaagtcg cgccgctggc ctatatgcgt ggtcgtacgc tgaacgacgc
     3781 gtttatcatt ctcgatgaga gccagaacac taccatcgaa cagatgaaga tgttcctgac
     3841 ccgtatcggt tttaactcaa aagcggttat caccggcgac gtcacacaga tcgacctgcc
     3901 gcgtaatact aaatcaggct tacgtcatgc cattgaagtg ctggccgatg tcgaagagat
     3961 cagctttaac ttcttccaca gcgaagacgt ggttcgtcac ccggtggtgg cgcgtatcgt
     4021 taacgcttat gaagcctggg aagaagcaga acaaaaacgt aaagcggcgc tggcagcaga
     4081 acgcaagcgc gaagaacagg aacaaaaatg agtcaggtga tcctcgattt acaactggca
     4141 tgtgaagata attccgggtt accggaagag agccagtttc aaacatggct gaatgcggtg
     4201 atcccgcagt ttcaggaaga gtcggaagtg actattcgcg tggtcgatac cgccgaaagc
     4261 cacagtctga atctgaccta tcgcggtaag gataagccga ccaacgtgct ctccttcccg
     4321 tttgaagtgc cgcctggcat ggagatgtcg ctactgggcg atctggttat ctgccgtcag
     4381 gtggttgaga aggaagctca ggagcaaggc aaaccactgg aggcgcactg ggcgcatatg
     4441 gtggtgcacg gcagtctgca tttgttaggt tacgatcaca ttgaagatga cgaagcagaa
     4501 gaaatggaag ccctcgaaac agagattatg cttgctctgg gctatgagga tccgtacatt
     4561 gccgagaaag aataatttgc caggcatttg actggcagac gtccccgccg ttggtgatta
     4621 acgacggcgt tttgattaac taacatgaga acccatacga cgccatgagc gacgacaatt
     4681 cacacagtag tgacacgata agcaacaaga agggattttt ctccctgtta ctcagccaac
     4741 ttttccacgg tgaaccgaaa aaccgtgacg aactgctggc gctgatccgt gattccgggc
     4801 agaacgacct tatcgacgaa gatacgcgcg atatgctcga aggggtgatg gacatcgcag
     4861 accaacgcgt tcgcgacatc atgatccccc gctcccagat gattaccctg aaacgcaacc
     4921 agacgctgga cgaatgcctt gatgtcatca tcgagtccgc ccactcacgt ttcccggtga
     4981 ttagcgaaga caaagatcac attgaaggga ttctgatggc gaaagacttg ctgccgttta
     5041 tgcgcagcga tgctgaagcc ttcagcatgg acaaagtgtt acgtcaggcg gttgtcgttc
     5101 ctgaaagtaa gcgcgtagac cggatgctga aagagtttcg ctctcagcgt taccacatgg
     5161 cgatcgttat tgacgaattc ggtggggttt ccggtctggt gaccattgaa gacatcctgg
     5221 aactgattgt tggtgagatt gaagacgagt atgacgaaga agatgatatc gacttccgtc
     5281 agctgagtcg tcatacctgg accgtgcgcg cactggcttc cattgaagac ttcaacgaag
     5341 cgttcggcac ccactttagc gatgaagaag tcgacactat cggtggtctg gtgatgcagg
     5401 cgtttgggca tcttccggcg cgtggcgaaa ctatcgacat cgacggttac cagttcaaag
     5461 tggcgatggc cgatagtcgg cgtattattc aggttcatgt caaaatcccg gatgactcac
     5521 cccagccaaa gctggatgaa taaaaccgaa actggataga taactacatg gcttttgcct
     5581 cattaattga acgccagcgc attcgcctgc tgctggcgtt attattcggt gcctgcggaa
     5641 cgctggcctt ctctccttac gacgtctggc ctgcggcgat tatttcgctg atggggcttc
     5701 aggcgttgac ctttaaccgt cgcccactcc agtctgccgc tattggcttt tgctggggat
     5761 ttggcctctt tggcagcggt attaactggg tctatgtcag catcgcgacc tttggcggga
     5821 tgcctggccc ggttaacatc ttcctggtgg tgctgctggc ggcgtatttg tcgctgtata
     5881 ccggactgtt tgctggcgtg ctgtcgcgtc tgtggccgaa aaccacctgg ctgcgcgtgg
     5941 cgattgccgc ccctgctctc tggcaagtga ccgagtttct gcgcggttgg gtgctgactg
     6001 gcttcccgtg gttacagttc ggctatagcc agattgatgg cccgttaaaa gggctggcac
     6061 cgctaatggg cgtggaagcc attaacttcc tgctgatgat ggttagtggc ctgctggcac
     6121 tggcgttggt caaacgcaac tggcgtccgc tggtggtggc cgtcgtgctg tttgccctac
     6181 ccttcccgct gcgttacatc caatggttta ccccgcaacc ggagaaaacc attcaggttt
     6241 cgatggttca gggcgatatt ccgcaatcgc tgaaatggga cgaaggccag cttcttaata
     6301 cgctaaagat ttactacaac gcaacggcac cgctgatggg caaatcatcg ttgattatct
     6361 ggccggagtc ggcgataacc gatctggaaa ttaatcagca accgttcctc aaagcactgg
     6421 acggtgagtt gcgtgataaa ggtagctcgc tggtaaccgg gattgtcgac gcgcgtctca
     6481 ataagcagaa ccgctacgat acctacaaca ccatcatcac gctgggtaaa ggtgcaccgt
     6541 acagctacga atcagccgat cgctataaca aaaaccatct ggtgccgttt ggcgagtttg
     6601 tcccgctgga gtcgattctg cgtccgttag caccgttctt tgatctgccg atgtcgtcgt
     6661 tcagccgtgg gccatatatc cagccgccgc tgtcggcaaa tggtattgag cttactgcgg
     6721 ctatttgcta cgagatcatt ctcggcgagc aagtgcgcga taacttccgc ccggataccg
     6781 actacctgct gactatctcc aacgatgcgt ggtttggtaa atctattggt ccatggcaac
     6841 acttccagat ggcgcgaatg cgtgcgctgg agctggcgcg cccactgttg cgcagcacca
     6901 acaacggcat tacggcggtg attggcccgc agggtgagat tcaggcgatg atcccgcagt
     6961 tcacccgcga ggtgttaacc actaacgtga cgccgaccac cggactcaca ccatacgcac
     7021 gtaccggcaa ctggccgctg tgggtgctga cggcactgtt tggttttgct gctgtgttga
     7081 tgagtctgcg tcagcgacgt aaataatccc ttcttatcgt gccggaatac atcccatatt
     7141 tcggcacgct caccggatgc ggcatcagtg cattatccgg ataacaatat tctcctatcc
     7201 aactttggca catctattgc tttgttatac aaggcaaagc ttgaaccagc atcagtacaa
     7261 cccggttgca ccacagcaat ccaccggtag caccaaaaga gtgcatttta agaattttgc
     7321 ttcagaatgg tgcggtgcac ctcgcaaaaa caaacaataa aacatcaata tctttacatt
     7381 tagccaaact aaatgttacc taacaggcac aacactgcac aataaagttg cagacgataa
     7441 caacacaaac actcacaacg ggtatccatg cgttcttaac gcagaagata aaggagttgg
     7501 atatgcaatt acgtaaacct gccacagcaa tcctcgccct ggcgctttcc gcaggactgg
     7561 cacaggcaga tgacgccgcc ccggcagcag gcagtacgct ggacaaaatc gccaaaaacg
     7621 gtgtgattgt cgtcggtcac cgtgaatctt cagtaccttt ctcttattac gacaatcagc
     7681 aaaaagtggt gggttactcg caggattact ccaacgccat tgttgaagca gtgaaaaaga
     7741 aactcaacaa accggacttg caggtaaaac tgattccgat tacctcacaa aaccgtattc
     7801 cactgctgca aaacggcact ttcgattttg aatgtggttc taccaccaac aacgtcgaac
     7861 gccaaaaaca ggcggctttc tctgacacta ttttcgtggt cggtacgcgc ctgttgacca
     7921 aaaagggcgg cgatatcaaa gattttgccg acctgaaagg caaagccgta gtcgtcactt
     7981 ccggcactac ctctgaagtt ttgctcaaca aactgaatga agagcaaaaa atgaatatgc
     8041 gcatcatcag cgccaaagat cacggtgact ctttccgcac cctggaaagc ggtcgtgccg
     8101 ttgcctttat gatggatgac gctctgctgg ccggtgaacg tgcgaaagcg aagaaaccag
     8161 acaactggga tatcgtcggc aagccgcagt ctcaggaagc ctacggttgc atgttgcgta
     8221 aagacgatcc gcagttcaaa aagctgatgg atgacactat cgctcaggtg cagacctccg
     8281 gtgaagcgga aaaatggttt gataaatggt tcaaaaatcc aattccgccg aaaaacctga
     8341 acatgaattt cgaactgtca gacgaaatga aagcactgtt caaagaaccg aatgacaagg
     8401 cactgaacta attacaagaa ccaggggcgg aaattccagc cctctcgatt gttacgtagc
     8461 acggacagac tatacgcctg atggtcgttc cccatcgggc ctgaaaaccg caatacgctg
     8521 ggtaacaatc ttcgagggta gcagttaacg ctgctaccct cttttttctg gagtagattt
     8581 atgtctatag actggaactg gggtattttt ttacaacaag ccccgttcgg caataccacc
     8641 tatcttggtt gggtctggag tggttttcag gtgacgatcg ctttgtcgat ctgcgcctgg
     8701 attatcgctt tcctcgtcgg ttcatttttc ggcattttac gtaccgttcc aaaccgtttt
     8761 ctctccggtc tgggtacgtt gtatgtcgaa ctgttccgca acgtgccgtt gattgtgcaa
     8821 ttctttacct ggtatctggt gatcccggag ctgctgccgg agaaaatcgg catgtggttt
     8881 aaggccgagc tggatcccaa tattcagttt ttcctttcat ccatgctctg cctggggctg
     8941 tttaccgccg cccgtgtttg cgaacaggta cgcgccgcta ttcagtcgct accgcgtggg
     9001 cagaagaatg ccgcgctggc gatgggcctg acgctaccgc agacttatcg atacgtactg
     9061 ctgcctaatg cttatcgcgt tatcgtcccg ccgatgactt ccgagatgat gaacctggtg
     9121 aaaaactccg ccatcgcctc gaccatcggt ctggtggata tggcggcgca ggcgggtaaa
     9181 ttgctcgact actcggctca cgcctgggag tcgtttaccg ccatcacgct tgcgtatgtg
     9241 ttgattaacg cttttatcat gctggtgatg acgctggttg aacgtaaagt tcgcctgccc
     9301 ggcaacatgg ggggcaaata atgtacgagt ttgactggag ttccattgtt ccttccctgc
     9361 catatctgct cgacgggctg gtgatcaccc tgaaaatcac cgtcacggca gtcgtgattg
     9421 gtattttgtg gggtaccatg ctggcggtga tgcgcttgtc cagcttcgcg cctgtttcgt
     9481 ggtttgccaa agcctacgtt aacgtattcc gctcgattcc tttagtcatg gttttgctgt
     9541 ggttttactt gattgtgccg ggatttctgc aaaatgtgct gggattatcg ccaaaaaatg
     9601 atattcgttt aatttcggcg atggtggcgt tttcgatgtt tgaagcggcc tactattcag
     9661 agattatccg tgccggtatt caaagtattt cccgtggtca gtcgagcgcc gcgctggcgt
     9721 tggggatgac tcactggcag tcgatgaaac tgattattct gccgcaggct ttccgcgcga
     9781 tggtgccgct gctgctcact cagggcatcg tactgttcca ggatacctcg ctggtgtatg
     9841 tgttatctct ggccgatttc ttccgtaccg cctcaaccat tggtgagcgt gatggtacgc
     9901 aggtcgagat gatcctgttt gccggatttg tttatttcgt tattagcctt agcgcgtcgt
     9961 tgttggtcag ctacttgaaa agaaggacag cataatgatt accctgaaaa atgtttcaaa
    10021 atggtatggt cactttcagg tgctgaccga ctgctcaacc gaagtgaaaa aaggcgaagt
    10081 ggtggtggtt tgcggcccgt ctggttccgg caaatcaacg ctgattaaaa ccgtcaacgg
    10141 cctcgaacca gtgcagcaag gtgaaatcac cgtcgatggt atcgtggtta acgacaagaa
    10201 aaccgatctg gcaaagctgc gttcccgcgt cgggatggta ttccagcatt tcgagctgtt
    10261 ccctcatctg tcgattatcg aaaacctgac cctggcgcag gtgaaagtgc ttaaacgcga
    10321 taaagcgccc gctcgtgaaa aagcattgaa attgctagag cgcgtgggac tttccgcaca
    10381 tgccaataaa tttccggcgc agctttccgg cggtcagcag cagcgtgtgg caatcgctcg
    10441 cgcattgtgt atggatccta ttgcgatgct gttcgacgaa ccgacatcgg cgctggatcc
    10501 ggagatgatc aacgaagtgc tggacgtgat ggtggaactg gcgaacgaag gaatgaccat
    10561 gatggtggtg acccacgaaa tgggctttgc ccgcaaagtg gcgaatcggg tgatctttat
    10621 ggacgagggt aaaattgtcg aagactcgcc gaaagacgct ttcttcgatg atccgaaatc
    10681 ggaccgcgca aaagacttcc tcgcgaaaat cctgcattaa tcactctggc gcgcataccc
    10741 aattgcgcgc catctcacgc tttatcgttt cgcccctcgt atctgttaca tcctggcgtt
    10801 acccttgtcg caaagaagca cacaacaagg agcaacaatg gcactgccaa ttctgttaga
    10861 ttgcgaccca ggtcatgatg acgctatcgc aatagttctc gccctcgcct caccagagct
    10921 tgatgtcaaa gcaattacgt cttccgccgg aaaccagaca ccagaaaaaa ccttacgcaa
    10981 tgttctgcgt atgctgacct tgcttaatcg caccgatatt ccggtggcag gcggcgcggt
    11041 aaaaccgtta atgcgtgagt tgattatcgc ggacaatgtg cacggcgaaa gcggtctcga
    11101 cggcccggca ttaccggaac cgacgttcgc accgcaaaac tgtacggcgg tggagctgat
    11161 ggcgaaaacg ctgcgcgaaa gtgcggaacc tgtcacgatt gtttctaccg gaccgcaaac
    11221 taacgttgcc ttgctgctca atagccatcc ggaactgcat agcaaaattg cccgtatcgt
    11281 gattatgggc ggcgcaatgg ggcttggtaa ctggacgcct gcggctgaat ttaatattta
    11341 cgttgacccg gaagcggcag aaattgtctt ccagtcaggg atcccggtgg tgatggccgg
    11401 tctggatgtt actcataaag cgcaaatcca cgttgaagac accgagcgtt tccgcgcgat
    11461 tggcaaccct gtttcaacca ttgttgccga actgctggat ttcttcctcg aatatcataa
    11521 agacgaaaaa tggggctttg tcggcgcacc gctgcatgac ccatgcacca tcgcctggct
    11581 gttgaaaccc gagttgttta ccactgttga gcgctgggtt ggcgtggaaa cacaggggaa
    11641 atatacccag ggtatgacgg ttgttgatta ttattatctg actggcaata aaccgaatgc
    11701 cactgtaatt gtcgatgttg atcgtcaggg ctttgttgat ttactggctg atcgtctgaa
    11761 attttacgct taacactcgt cattgattaa gatcaccacg cgttacctgg gtggtcttaa
    11821 tctatttatc tctttttctt tcaagatata aatgcaatgg ataatgcaga actcgccata
    11881 ggtatcgatc tcggcactac caatagttta attgccgtct ggaaagacgg tgccgcgcaa
    11941 ttaattccaa ataagttcgg tgaatattta acaccatcca taattagcat ggatgaaaat
    12001 aatcatattt tagtcggaaa accggctgta tcacggcgta cttcgcatcc ggataaaacg
    12061 gcagcgttat ttaaacgtgc aatgggcagt aataccaact ggcggttagg cagcgacaca
    12121 tttaacgcgc cagaactgtc ctctttggta ttacgctcat taaaagaaga tgccgaagaa
    12181 tttctgcaac gtccgattaa agatgtggtg atctccgttc cggcttattt cagcgatgaa
    12241 caacgcaagc atacccgttt agcagcggac ttagccgggt taaatgcggt acgcttaatt
    12301 aatgaaccca cagcagctgc gatggcgtat ggcctgcata cccaacaaaa tacccgttcg
    12361 ctggtgtttg atctcggtgg cggcacgttt gacgttacgg tgcttgagta cgccacgccg
    12421 gtgattgaag ttcacgcctc cgctggcgac aacttcctcg gtggcgaaga ttttacccat
    12481 atgttggtcg atgacgtttt aaagcgcgcg gatgtcacaa ggaccacgct taacgagagt
    12541 gaactggcag ccttgtacgc ctgtgtggaa gcggcaaaat gtagcaatca atcgccaatg
    12601 cacattcgct ggcagtacca ggatgaaacg cgggaatgcg aattttacga gaacgaactg
    12661 gaagatttgt ggttgccgct gctcaatcgc ttgcgagtgc cgattgaaca ggcgttgcgc
    12721 gatgcgcgtc tgaagccgag tcaaatcgac agtctggtgc tggttggcgg cgcgtcacaa
    12781 atgccgctgg tgcagcgaat cgccgtgcgt ctgtttggca aattaccgta tcaaagttac
    12841 gatccgagca ccattgtcgc gctgggcgca gcaatccagg ccgcctgccg cttacgcagt
    12901 gaagatattg aagaagtgat cctcactgat atttgccctt actcgttggg cgttgaggtt
    12961 aaccgccagg gcgtttccgg cattttctcg ccgattattg aacgaaacac cactgtgccc
    13021 gtgtcgcgtg tagaaactta ttcaaccatg cacccggaac aggattcaat tacggttaac
    13081 gtctatcagg gagaaaacca caaagttaaa aacaacattc tggtggaatc cttcgatgtg
    13141 ccgttgaaga aaaccggggc ttatcagtcg attgatattc gctttagtta tgatatcaac
    13201 gggttgcttg aagttgacgt gcttctggaa gacggcagcg ttaagtccag agtgattaac
    13261 cacagcccgg taacattgag cgcgcagcag attgaagaga gtcggacgcg gttatccgca
    13321 ttgaaaattt atccgcgcga tatgctcatc aatcgcacct ttaaagccaa actggaagag
    13381 ttgtgggcgc gggcgctggg tgacgagcga gaagagattg gccgggtgat caccgatttt
    13441 gatgcggcgc tgcagtcaaa cgatatggcc cgcgtcgatg aggtcaggcg gcgggcgagc
    13501 gtttatttag ccattgagac ctcataatca cggctgccgg atgcggcgta aatgctgcat
    13561 ccggcatcgg cgcaaaaaac acatcgtcaa ctaaacaaca tcgcgatttt gccgagagca
    13621 taacctataa ccagcaccgt taaactgatt ttgataatgc tggtaatgct ctgtggtttt
    13681 tcaccctcat catttttttc ttcatggttt tcaggtgttg gctcatcgac atcttcatcc
    13741 tccggctcga gttttacttt cgtcaacagc atccgggtga taatggcctc taatggatga
    13801 gcatctccgc cttccagcct tgtattaaga cgggccagca tctcttgttt cggaaacgct
    13861 tcaagtacta cctgttccgc tacccgatac aaatccagta acggtgaatc ttcttctgtc
    13921 tcgctaccga ctctcgattt ccaccacgcc catgctttgc gtttgaagaa taacgactca
    13981 ataaacgatt gacctaacgg cgataatttt ccgctatgta aaatttccgc ccataacagc
    14041 cgcgtccggg cgctctcacc aggaacatat ttcagcggtt gaccttgcgc atctaccatg
    14101 ctccgcgctt cgataaccat cactaataac gcgaaataat ccgggcaatg acgcttgctc
    14161 cactgcaaca acaaattatc agcttgtgta gatgggtaac tttcccagta cgcgccgaga
    14221 tcggcaagca ggctttcccc ctcgccgcag tacaaacgtt gtgcacactg gtaataccgg
    14281 gcatcttcgc tttctggttc ttccgcttgc cactgtttgg ctacaggaat gagttccacc
    14341 atacctgttt gcaccgagct gtaccagcgc aacagtttgc gatgtaactt tgcatcatca
    14401 ggaatgaacc acggtccttc catcgccaaa aacgcagtga catagtcagg atggtaacgg
    14461 cagcaacgtt ccagcgcgaa gtaatattca acagtctggt tctgtaacgc gacgggatat
    14521 tccagaaggc taaggaaatc gaaacaatca ccagcctgaa tggcctcaag aaaagcctcc
    14581 ctttcttcct catccattcc ttcgctgtca ttaaacgaat gccagttcaa acgctctgcc
    14641 aacaaagaag cacaatttaa tgacagatat cgcgcttcta tagcgattgc acacagcggc
    14701 cagcgtaatt gatcgacatc ctccatgttc caggtattta attgctgaat aaatttctgc
    14761 caggcggagg gctgaaaacg atcactttct gaatccagta atgtcctgaa tgcacgtagt
    14821 atttcatgtt cagcggtggc atctttttct tcatcatctg cttcctcgac aggatttacc
    14881 gccagtcgca gcgcctcttc ataggcctgg cgtaatgctt taaacccctg cggatcggtt
    14941 tcgggatgac acaacggtaa gcgagcaaga taagcctggc ggataatgtc tatttgcgtc
    15001 gtgctttcaa tttcgaggat ttgccaacat gttttcatag gtcgctcaca tccttaagcg
    15061 ttaccgggca ttcaggctgg acagttaatg gctgccaggg aagcgtggca tagaaacggt
    15121 cgcttcccgc tgccagctct tcgaacatcc gactcccggc gctatcgtat ttgtacaggg
    15181 ttagcgcctc gcgtagcgaa gcgttgtctt cgcactgaga agattgccag tagagacgcc
    15241 ctagtgaata ggcagaaaaa tagtgcatcc agctgtgata gtaatggtga gctcgtgcat
    15301 agacgcgcga ttgcagtcat aaactctctt cttccgtcag ccagttgttg agcacaccga
    15361 cccgtgacag aaaccccatt cgaacataat cccaggcttt gattccccct tctccacagc
    15421 acatagcggt ttgggcaacg aattgggtat aaatcaaccc tctctccgag ccaccagcaa
    15481 ttaatgcttt ccactcttca ggggaagagc gttgccatgt cagatataag ccagctaaat
    15541 cattggcatg accatcatca gtcatctgat aaatagtctg aattaaatca tcacgattat
    15601 ttattcccca ggatgatgag agatcgaatt cttttatccc ggtatacatt tttggcgagt
    15661 catgcgcggg tttgcaactc tgattaagta tttccatggg cgcagaaagc gcaaaaagaa
    15721 gatattgttc ttccttattc attttttaat aataccttaa gtgcattaac tgactacttt
    15781 tttattattg atgagtataa acttgacgct ttcaaaataa aaagaaaatc gaagcattca
    15841 cacatgaata aaaaattaat gtatatattc gcaattttta tagttgcagc aattacctgt
    15901 attagccaac ccaagaaaac gacgttgcgt gataaagcca tggtgaatta tgcctttgat
    15961 tatttaagat caccaggcag tctaccgtta accacggcag ccacggagct ttccgcgatt
    16021 catggtcact caacatcgca atatcgcctt ggcgaatttt atcttcatgg tagcgacggt
    16081 aaaccactgg attatacaca ggcgagatac tggtatgagc aatcagcaga acaggaaaat
    16141 ccgcgcgcac aaagcaaact ggggtggatc tacctgaaag gcctgggagt caaacccgat
    16201 acccgtaaag caattctctg gtataaggaa gcagctgaac aagggtatgc tcatgctcaa
    16261 tatactttgg gtttgatcta cagaaatggc actggtatta atgttaacca ttatgaatct
    16321 caaaaatggt taaaactggc cgccaaacaa cattacaaaa atgcggaaag attacttgcc
    16381 ggacttcccg cacattaacg tcgactgtca cccgaagaga tggagtattt tccctatcaa
    16441 accggcaaag ataaaaatgt agaaaataat tttgatgacg cccagacagc ccagcccgcc
    16501 atcgtcaacg ggcattggct gcggttgcgg ctccggctcg acggtttcct gaccggtaaa
    16561 attcgccgcc tgaattaata atcgacgaat aattaatgct tccagtggac aacgatcttc
    16621 tgcatcaagg cggctcacaa tcggcattaa tgattcttct gtcggttggc tggcctgcac
    16681 taattgtttg gcaactaaat ataatggcca taagggatgt tctgtatcac gcgtgagtga
    16741 gatccgcggc ttacgtcctt tgtgcaaaac catctcgacc agtgaacgcc ccagcgcaga
    16801 gagtttttca tcgctgagta tttcgtataa atgaaaacgc gtacgggcgc tgtcgccggg
    16861 gacataaagt agcggttttc cctgatcgtt aaccagatca cgcgcttcaa tcatcatcac
    16921 cactaatggg taataatcga cccgatgctg acggcaccat tgcaacatta aagcatccgc
    16981 ctgggtggag ggatactcgc gccagtagta acaaagttct gccagcaggc tttcgccttc
    17041 tccacaatag acgcgttgtg cgtattcata ataacgcgca tcttcattat caggttcagc
    17101 ttcccgccac gaccgggcgt aatccaggag ttcggggatt cccaggcggc ttgcgctata
    17161 ccagcggagt aaatttctgt gcagttttgc atcatcgggg ataatcactg cgcgatgttg
    17221 cgctaaatac aatgtcagcc attctggatg cgatgaccaa atccgagcga acatttggta
    17281 aaaatcgatg gtgtcatttt gcacggcaac gggcagatgc agaatggtct ggaagttaaa
    17341 aacattcccc ttcgcaatgg catataaaaa ggattccagt tcttcttcgt ctatttcccc
    17401 gacgttttcc tcctgcaacc accgcaatct ttctgctaat aacaccacgc actcgaagga
    17461 taaatgggca gtgttcatgg cgattgtgca cagcgaccag cgtaattcat caatctcttc
    17521 catcgagcaa taatttaatt gctgaatgaa tcgctgccag gcggagggca gaaaacgttc
    17581 gctatcagag gcaagtaacg cgcgaaaggc gagcagaatt tcatgttctg ctacctcata
    17641 ttcttctggt tgccaaacag atttagcagg cgactgcgca atccgtagcg cctcctcata
    17701 cgcctgacga agttgtttaa aaccctgcgg atcggtttct ggatgaaagg acggtaacag
    17761 cgccagataa gcgcggcgga taatatcgac gtcagtcgtt tcctctatat cgaggatctt
    17821 ccagcaattt ttcataagtc gctcatatcc ttaagcgttt ccgggcatgt ggggtagtca
    17881 gccagtggtt gccaggacaa ggtagggtaa tacgcatcat cactggcaaa taattgataa
    17941 aacatatcat tgccggaatc gtcgtattct ttacgcgcta agaattcgcg taataaaggc
    18001 agatgatcat cttcgggaga ctgccaatat tgccgaccaa aggtataacc ggcaaagtat
    18061 tgccgccagt tactataata acgtagagcc cgtagatgaa tacgcgactg gatccataga
    18121 ctttcttctt cactcaacca gttattaagt acgcccattc gacttaaaaa acccatccgg
    18181 acataatccc atgctttaat tcctccctca ccacagcatt cggctgtgct ggcgacaaat
    18241 tgcgcataag cctgaccttg ctcatttaat tcagcaagat agtcacgcca ttcacatgga
    18301 gaataacgaa accagcggtg ataaaaccct gccaaccgag cggcatgacc gttctcgatc
    18361 attcggtgaa ttgtgcgcag caggtcatca cggtcatcaa ttccccatga ggaagaaagg
    18421 tcaaaaaagc atgtacctaa atacattttg ggggataaat gcgaaggtaa acagcattca
    18481 ttgcgtattt ccatcggagt ggaaagtgca aacaatattt tttgcgaccc catgtccata
    18541 taatttatcc ttaaacattc atttaatgaa tgtaaactac atttcgagac tcagttattt
    18601 atacttttct gacgcaacat aatacgcata aaataaggct tacgcaaaaa tggaacacat
    18661 aaaaatataa tatactttat ctacaaccat aaggaaatga taatgatttt tacgtcaagt
    18721 tgctgcgata atttatcaat agatgagatt atcgaacgtg ctgaaaaagg cgattgcgag
    18781 gctcaatata ttgttgggtt ttattataat cgcgatagcg caattgactc tccggacgac
    18841 gaaaaggcct tttactggct gaagctggcc gctgagcaag gtcattgtga agcacagtat
    18901 tccttagggc aaaagtatac cgaggataaa agccgtcata aagataatga gcaagccatc
    18961 ttctggctga aaaaagctgc cctacaaggc catactttcg cttccaacgc ccttggctgg
    19021 atactggatc gtggagaaga ccccaactat aaaaaagaag cagttacctg gtatcagata
    19081 gccgcggaga gcggaatgtc ttatgcgcaa aataatcttg gatggatgta cagaaatggc
    19141 aacggagtcg cacaagacta tacgctggca tttttctggt acaaacaagc tgcattacaa
    19201 ggccatagtt acgcacaaga caatctggcc gatctttata aagacggaga aggcgttgct
    19261 caaaacaaga cactcgccgc attctggtat ttgaaaagcg cacagcaggg taatcggcac
    19321 gcccagtttc aaattgcatg ggattataac gctggcgaag gggtggacca ggactataag
    19381 caagcgatgt actggtatct gaaggctgcc gctcaggaaa gcgtcggcgc ttacgtcaac
    19441 atcggttata tgtataaaca cggacaaggc gttgagaaag attatcaggc tgcctttgaa
    19501 tggtttacga aagccgctga atgcaatgac gccactgcct ggtataacct ggccattatg
    19561 tatcactacg gagaaggaag acctgtcgat ctccgacagg ctctcgacct gtatcgtaaa
    19621 gttcagtcat ccggaaccag agatgtcagt caggaaattc gtgagactga agatttactg
    19681 tagaaataaa aacaagggcc ggggaatttc attctgccca tattctcttt attgctgtgc
    19741 gaaatcagac tattacggct caaacgggcg tctctggaac tggtcataac cacatttcgg
    19801 gcatagcgtc agcacttccg gtgtgtagat cgggagatgg aagtgacatt tctcgcagac
    19861 cagatttccc agcccgacca cttctccgct gtgataaacc ccatgatgat tgaggtcctg
    19921 gaaaacttcg cgccattcaa gctgcgtttt atcggtgatg tctgccagct cctgccacaa
    19981 gctttcttta atcacccgca taaagacgct gtcagattct tctttcaggc tctcttcata
    20041 gctcatggcg aactcttcca ggtcacgtct gacagctcgc gtcagctcat cgatctcggt
    20101 tcgcgttaac tcccctgttt ttattacgcg ctcgcgcgcc tgttccacca gtgcgtcgat
    20161 atcacgttcg ccattgcgca ggcgttcgtt cagtgacgcg accagttcac ggtaatattg
    20221 agcaaccttg ttcatcgttt catctcccgg gtcgttaact gactataata atagacgttc
    20281 tttgttcctt gctttgctaa tacgcccaca aactctgtaa attaatgtac gaataatccg
    20341 ggcatcattt caacgtcgag tgcgcgaaag gctgttttga cgcggacgtt tgggctatgc
    20401 tatgcggatc tgaaaaacca cctcaacgct acatttgtag ccgtattgaa aacaggacca
    20461 ctggctgcca tgcaagagca ataccgcccg gaagagatag aatccaaagt acagcttcat
    20521 tgggatgaga agcgcacatt tgaagtaacc gaagacgaga gcaaagagaa gtattactgc
    20581 ctgtctatgc ttccctatcc ttctggtcga ctacacatgg gccacgtacg taactacacc
    20641 atcggtgacg tgatcgcccg ttaccagcgc atgctgggca aaaacgttct gcagccgatc
    20701 ggctgggacg cgtttggtct gcctgcggaa ggcgcggcgg tgaaaaacaa caccgctccg
    20761 gcaccgtgga cgtacgacaa catcgcgtat atgaaaaacc agctcaaaat gctgggcttt
    20821 ggttatgact ggagccgcga gctggcaacc tgtacgccgg aatactaccg ttgggaacag
    20881 aaattcttca ccgagctgta taaaaaaggc ctggtatata aaaagacttc tgcggtcaac
    20941 tggtgcccga acgaccagac cgtactggcg aacgaacaag ttatcgacgg ctgctgctgg
    21001 cgctgcgata ccaaagttga acgtaaagag atcccgcagt ggtttatcaa aatcactgct
    21061 tacgctgacg agctgctcaa cgatctggat aaactggatc actggccaga caccgttaaa
    21121 accatgcagc gtaactggat cggtcgttcc gaaggcgtgg agatcacctt caacgttaac
    21181 gactatgaca acacgctgac cgtttacact acccgcccgg acacctttat gggttgtacc
    21241 tacctggcgg tagctgcggg tcatccgctg gcgcagaaag cggcggaaaa taatcctgaa
    21301 ctggcggcct ttattgacga atgccgtaac accaaagttg ccgaagctga aatggcgacg
    21361 atggagaaaa aaggcgtcga tactggcttt aaagctgttc acccattaac gggcgaagaa
    21421 attcccgttt gggcagcaaa cttcgtattg atggagtacg gcacgggcgc agttatggcg
    21481 gttccgggtc acgaccagcg cgactacgag tttgcctcta aatacggcct gaacatcaaa
    21541 ccggttatcc tagcggctga cggctctgag ccagatcttt ctcagcaagc cctgactgaa
    21601 aaaggcgtgc tgttcaactc tggcgagttc aacggtcttg accatgaagc ggccttcaac
    21661 gccatcgccg ataaactgac tgcgatgggc gttggcgagc gtaaagtgaa ctaccgcctg
    21721 cgcgactggg gtgtttctcg tcagcgttac tggggcgcgc cgattccgat ggtgacgctg
    21781 gaagacggta ccgtaatgcc aaccccggac gaccagctgc cggtgatcct gccggaagat
    21841 gtggtgatgg acggcattac cagcccgatt aaagcagatc cggagtgggc aaaaactacc
    21901 gttaacggta tgccagcgct gcgtgaaacc gacactttcg acacctttat ggagtcctcc
    21961 tggtactatg cgcgctacac ttgcccagag tacaaagaag gtatgctgga ttccgaagcg
    22021 gctaactact ggctgccggt ggatatctac attggtggta tcgaacacgc cattatgcac
    22081 ctgctctact tccgcttctt ccacaaactg atgcgtgatg caggcatggt gaactctgac
    22141 gaaccagcga aacagttgct gtgtcagggt atggtgctgg cagatgcctt ctactatgtt
    22201 ggcgaaaacg gcgaacgtaa ctgggtttcc ccggttgatg ctatcgttga acgtgacgag
    22261 aaaggccgta tcgtgaaagc gaaagatgcg gcaggccatg aactggttta taccggcatg
    22321 agcaaaatgt ccaagtcgaa gaacaacggt atcgacccgc aggtgatggt tgaacgttac
    22381 ggcgcggaca ccgttcgtct gtttatgatg tttgcttctc cggctgatat gactctcgaa
    22441 tggcaggaat ccggtgtgga aggggctaac cgcttcctga aacgtgtctg gaaactggtg
    22501 tacgagcaca cagcaaaagg tgatgttgcg gcactgaacg ttgatgcgct gactgaagat
    22561 cagaaagcgc tgcgtcgcga tgtgcataaa actatcgcta aagtgaccga tgatatcggc
    22621 cgtcgtcaga ccttcaacac cgcaattgcg gcgattatgg agctgatgaa caaactggcg
    22681 aaagcaccga ccgatggcga gcaggatcgc gctctgatgc aggaagcact gctggccgtt
    22741 gtccgtatgc tcaacccgtt caccccgcac atctgcttca cgctgtggca ggaactcaaa
    22801 ggcgaaggcg atatcgacaa cgcgccgtgg ccggttgctg acgaaaaagc gatggtggaa
    22861 gactccacgc tggtcgtggt gcaggttaac ggtaaagtcc gtgccaaaat caccgttccg
    22921 gtggacgcaa cggaagaaca ggttcgcgaa cgtgctggcc aggaacatct ggtagcaaaa
    22981 tatcttgatg gcgttactgt acgtaaagtg atttacgtac caggtaaact cctcaatctg
    23041 gtcgttggct aagcgcggga ggaagcgtgc gatatctggc aacattgttg ttatctctgg
    23101 cggtgttaat caccgccggg tgtggctggc atctgcgtga caccacgcag gttccttcca
    23161 ctatgaaggt catgatcctg gactcaggcg atccgaacgg gccattaagc cgtgcggtgc
    23221 gtaaccagtt acgtctgaat ggtgtcgagt tgcttgataa agaaaccacg cgtaaggacg
    23281 ttccatccct gcgtttgggt aaagtgagca tcgcgaaaga taccgcatcg gtattccgta
    23341 acggtcaaac agcagagtat cagatgatca tgacggtgaa tgcgaccgtg ttgatccccg
    23401 gccgtgatat ctacccgatt aacgccaaag tcttccgttc attcttcgat aacccgcaaa
    23461 tggcgttagc gaaagataac gaacaagaca tgatcgtaaa agagatgtac gaccgtgctg
    23521 ccgaacagct gattcgtaag ctgccaagca tccgtgctgc ggatattcgt tccgacgaag
    23581 aacagacgtc gaccacaacg gatactccgg caacgcctgc acgcgtctcc accacgctgg
    23641 gtaactgatg attcggttgt acccggaaca actccgcgcg cagctcaatg aagggctgcg
    23701 cgcggcgtat cttttacttg gtaacgatcc tctgttattg caggaaagcc aggacgctgt
    23761 tcgccaggta gctgcggcac aaggattcga agaacaccac actttttcca ttgatcccaa
    23821 cactgactgg aatgcgatct tttcgttatg ccaggctatg agtctgtttg ccagtcgaca
    23881 aacgctattg ctgttgttac cagaaaacgg accgaatgcg gcgatcaatg aacaacttct
    23941 cacactcacc ggacttctgc atgacgacct gctgttgatc gtccgcggta ataaattaag
    24001 caaagcgcaa gaaaatgccg cctggtttac tgctcttgcg aatcgcagcg tgcaggtgac
    24061 ctgtcagaca ccggagcagg ctcagcttcc ccgctgggtt gctgtgcgcg caaaacagct
    24121 caacttagaa ctggatgacg cggcgaatca ggtgctctgc tactgttatg aaggtaacct
    24181 gctggcgctg gctcaggcac tggagcgttt atcgctgctc tggccagacg gcaaactgac
    24241 attaccgcgc gttgaacagg cggtgaatga tgccgcgcat ttcacccctt ttcattgggt
    24301 tgatgctttg ttgatgggaa aaagtaagcg cgcgttgcat attcttcagc aactgcgtct
    24361 ggaaggcagc gagccggtta ttttgttgcg cacattacaa cgtgaactgt tgttactggt
    24421 taacctgaaa cgccagtctg cccatacgcc actgcgtgcg ttgtttgata agcatcgggt
    24481 atggcagaac cgccggggca tgatgggcga ggcgttaaat cgcttaagtc agccgcagtt
    24541 acgtcaggcc gtgcaactcc tgacacgaac ggaactcacc ctcaaacaag attacggtca
    24601 gtcagtgtgg gcagagctgg aagggttatc tcttctgttg tgccataaac ccctggcgga
    24661 cgtatttatc gacggttgat atgaaatctt tacaggctct gtttggcggc acctttgatc
    24721 cggtgcacta tggtcatcta aaacccgtgg aaacgctggc gaatttgatt ggtctgacgc
    24781 gggtcacaat catccctaat aatgttcctc cgcatcgtcc ccagccggaa gcgaacagcg
    24841 tgcagcgtaa acacatgctt gaactggcga ttgccgacaa gccattattt actcttgatg
    24901 aacgcgagct aaagcgcaat gccccctctt acactgcgca aacactgaaa gagtggcggc
    24961 aggaacaagg accggacgtg ccgctggcgt ttattattgg tcaggattca ttgctgacct
    25021 ttccgacctg gtacgaatac gaaacgatac tcgacaatgc acatttgatc gtctgtcggc
    25081 gtccaggtta cccacttgaa atggcgcaac cgcaatacca gcaatggctg gaagatcatt
    25141 tgacacataa cccggaagat cttcaccttc agcctgccgg taaaatttat ctggctgaaa
    25201 cgccgtggtt taacatctcg gcgaccatca tccgcgaacg tttgcaaaac ggtgaatcgt
    25261 gtgaggattt attgccggaa ccggtattga cttacattaa ccaacaaggc ttgtatcgct
    25321 gatacctgtc gttacggagg gcatgatgcg actgtggtta attcgtcatg gcgaaacaca
    25381 agcgaatgtc gatggtctgt acagcggtca tgcgcccaca cccctgaccg cacgcggtat
    25441 tgagcaagcg caaaatctgc atacactact agatgatgtt tcctttgacc tggttttatg
    25501 cagtgaactg gaacgggcac agcataccgc gcgactggtt ctcagtgacc gccagctccc
    25561 cgttcatatc atccctgaac tcaacgaaat gttttttggc gactgggaga tgcgacatca
    25621 tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg tggtgcaatg actggcagca
    25681 tgctatcccc acgaacggtg aaggatttca ggcattttcg caacgtgtgg aacgctttat
    25741 cgcaaggctt agtgaatatc agcactatca gaatatttta atcgtcagcc atcagggcgt
    25801 actgagtctg ttaattgctc gtttaattgg catgcctgcc gagtctatgt ggcattttcg
    25861 cgttgactag gggtgttgga gcgccattga tatcaaccaa aaattcgcga cgctacgcgt
    25921 cctcaatagc cgtgccatcg gggtcgagaa tgcatgactt ttctgttttt ttacgggtaa
    25981 gccgcaacga ccattgacag ccccgggcag gctgatattc tccgcagcca gactttttcc
    26041 gccagacacg actttgtaga aattgtttta caaaaatggc gatgcaatct gcggcgcggg
    26101 gtgggatgat agcccacttt cgaaagccga ttcggcgaca attgtcccga aatcgcctct
    26161 ggttcaggta tactgacaga ccatttttat ctatttgatt cacccagggg gaaaacttgc
    26221 agggtaaagc actccaggat tttgttatcg acaaaattga tgacctcaaa ggtcaggaca
    26281 tcatcgcctt agacgttcaa ggcaaatcca gcatcaccga ctgcatgatc atctgtacgg
    26341 gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt tgtgcaggag tctcgcgcag
    26401 cgggcctgtt accgctcggc gtagaaggtg aaaacagcgc cgactggatt gtcgtggatt
    26461 tgggcgatgt gattgtccat gtcatgcagg aagagagccg tcgcctgtat gaactggaaa
    26521 aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt gggaacgaaa atgccggact
    26581 gggtacaaac cggttttacc gagtacctgc gtcgttttcc gaaagatatg cccttcgagc
    26641 tgattgaaat tccggccggc aaacgcggca agaatgcgga catcaagcgc atactcgaca
    26701 aagagggtga gcagatgttg gcggccgcag gcaaaaaccg cattgtcacc ctcgatattc
    26761 caggcaagcc ctgggatacg ccgcagttag ccgctgagct ggaacgctgg aagctggatg
    26821 gtcgcgacgt aagtctactg attggcgggc ctgaagggtt gtcgcctgcc tgtaaagcgg
    26881 cggctgagca gagctggtcg ctgtcggcgc ttaccctccc ccatccgctg gttcgcgtgc
    26941 tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac caaccatcct tatcaccgtg
    27001 agtgataagg gagctttgag tagaaaacgc agcggatgaa actacagaac tcttttcgcg
    27061 actatacggc agagtccgcg ctgtttgtgc gccgggcgct ggtcgccttt ttggggattt
    27121 tgctgctgac cggcgtgctt atcgccaacc tgtataatct gcaaattgtt cgctttaccg
    27181 actaccagac ccgctctaat gaaaaccgca ttaagctggt gcctatcgcg cccagccgcg
    27241 gcattatcta cgaccgtaac ggtatccctc tggccctcaa ccgcactatc taccagatag
    27301 aaatgatgcc ggagaaaatc gataacgtgc agcaaacgct ggacgctttg cgcagcgtgg
    27361 tagatctgac cgatgacgat attgctgcat tccgaaaaga gcgcgcacgt tcacaccgtt
    27421 tcacctctat tccggtgaaa actaacctga ccgaagtaca agtagctcgc tttgccgtca
    27481 atcagtaccg ttttccgggt gtcgaagtta aaggctataa acgtcgttac tatccttacg
    27541 gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat caacgataaa gacgtcgaac
    27601 gcctgaataa tgacggcaaa ctggccaact atgcggcaac gcatgatatt ggtaagctgg
    27661 gcattgagcg ttactatgaa gacgtgctgc acggtcagac cggttatgaa gaggttgaag
    27721 ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt accaccgcaa gccggacacg
    27781 atatttacct gacgctggat ctcaaactcc agcaatatat tgaaacgctg ctggcgggta
    27841 gccgcgcagc tgtggttgtt accgatccgc gtacaggtgg ggtgctggcg ctggtttcca
    27901 cgcctagtta tgacccaaac ctgtttgttg acggtatctc cagcaaagat tattccgcct
    27961 tgctgaacga cccgaataca ccgctggtga accgcgccac acagggggtt tatcctcccg
    28021 cgtctacagt taaaccctat gtggcggttt cggcattaag cgccggggtg atcacgcgca
    28081 atacgacgct gtttgaccca ggctggtggc aactaccagg gtcggaaaaa cgttatcgtg
    28141 actggaaaaa atggggccac gggcgtctga atgtcacaag atcgctggaa gaatctgcgg
    28201 ataccttctt ctatcaggtg gcctacgata tggggatcga tcgcctctcc gaatggatgg
    28261 gtaaattcgg ttatggtcat tacaccggta tcgacctggc ggaagaacgt tccggcaaca
    28321 tgcctacccg cgaatggaaa cagaaacgct ttaaaaaaca gtggtatcag ggtgacacca
    28381 ttccggttgg tatcggccag ggttactgga cagcgacccc aatccagatg agtaaggcac
    28441 tgatgatcct gattaatgac ggtatcgtga aggttccgca tttgctgatg agcaccgccg
    28501 aagacggcaa acaggtgcca tgggtacaac cgcatgaacc gcccgtcggc gatattcatt
    28561 ccggttactg ggagctggcg aaagacggta tgtacggtgt tgctaaccgc cctaatggta
    28621 cggcgcataa atactttgcc agcgcaccgt acaaaattgc ggcgaaatct ggtaccgctc
    28681 aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca caaaattgcc gagcgtttac
    28741 gtgaccacaa actgatgacc gcctttgcgc catacaacaa tccgcaagtg gctgtcgcca
    28801 tgattctgga gaacggcggc gcgggtccgg cggttggtac gctgatgcgc cagatcctcg
    28861 accacattat gctgggtgat aacaacaccg atctgcctgc ggaaaatcca gcggttgccg
    28921 cagcggagga ccattaatca tgacggataa tccgaataaa aaaacattct gggataaagt
    28981 ccatctcgat cccacaatgc tgctgatttt actggcattg ctggtttaca gcgccctggt
    29041 tatctggagc gccagcggtc aggatattgg catgatggag cgtaaaatcg gccaaatcgc
    29101 gatgggtctg gtcatcatgg tggtgatggc gcaaattcct ccacgcgttt atgaaggctg
    29161 ggccccctat ctctatatca tctgtattat tttgctggtg gcggtagatg ctttcggagc
    29221 catctctaaa ggcgctcaac gctggctgga cctcggtatt gttcgttttc agccgtcgga
    29281 aattgccaaa atagccgtac cactgatggt tgcgcgcttt atcaaccgcg acgtttgccc
    29341 gccatcgttg aagaacactg gcatcgcgct ggtgctgata tttatgccca cgctgctggt
    29401 ggctgcacag cctgacctag gaacatcaat cctcgttgcg ctttccggtc tgtttgtact
    29461 gttcctctct ggccttagct ggcgtctgat tggcgtcgca gtagtgctgg tagcggcgtt
    29521 cattccgatt ctgtggttct tcctgatgca tgattaccag cgccagcgcg taatgatgct
    29581 cctggacccg gaatcagacc cactcggcgc gggctatcac attattcagt ctaaaattgc
    29641 tattggctcc ggcggattac gcggcaaagg ctggctgcac ggcactcagt cacagcttga
    29701 atttctcccc gaacgccata ctgactttat cttcgcggta ctggcggaag agctgggatt
    29761 agtgggcatt ctgattctgc tcgctctcta cattctgctg atcatgcgcg ggctgtggat
    29821 agccgccaga gcgcaaacca cctttggtcg cgtcatggct ggtggcttaa tgctgatatt
    29881 attcgtttat gtcttcgtaa atattggtat ggtaagcggt attctgccgg ttgtaggggt
    29941 tccgctccca ctggtcagtt atggaggatc ggcgctaatt gtgctgatgg ctgggttcgg
    30001 gattgtaatg tcaatccaca cccacaggaa aatgttgtcg aaaagcgtgt aagaggtgcg
    30061 caatgcgtaa gcagtggctc gggatctgca tcgcggcagg aatgctcgcg gcatgtacaa
    30121 gcgatgatgg tcagcaacag acggtaagtg taccgcagcc tgcggtatgt aacggcccca
    30181 tagttgaaat tagcggggcg gacccgcgtt tcgaaccact gaacgcgacg gcaaatcagg
    30241 attaccagcg cgacggtaaa agctacaaaa tcgtgcagga tccgtctcga tttagccagg
    30301 cgggactggc ggcaatctat gatgccgaac caggcagtaa cctgacggcc tctggcgaag
    30361 ctttcgatcc gacaaagctg acggcggccc atccaacgct tccgatcccc agctacgcca
    30421 gaatcactaa cctggctaac gggcgaatga tcgtggtgcg cattaatgat cgcggccctt
    30481 acggcaacga ccgcgttatt tcgctttctc gcgcggcagc tgaccgtctt aacacgtcaa
    30541 acaacaccaa agttcgtatc gatccgatta ttgtcgccca ggatggttcg ctttctggtc
    30601 ctggtatggc ttgtaccaca gtcgccaaac agacttacgc cctgcctgca cctcccgatt
    30661 taagcggtgg cgcgggaaca agttcagtgt ctggcccgca gggtgacatt cttccggtca
    30721 gtaattcgac gctaaaaagc gaagatccga ccggcgcgcc ggtaaccagt agcggtttcc
    30781 tcggcgctcc aacgacctta gcgcctggtg tactggaagg cagcgaaccg acgcctgcgc
    30841 cacagcccgt tgttacagct ccgtcgacaa cgcctgcaac ctcgcctgca atggtgacac
    30901 cgcaagccgc ctcgcaaagc gccagcggca actttatggt gcaagtcggg gccgtaagcg
    30961 atcaggctcg tgcgcaacag taccaacagc aactgggaca gaagttcggc gtccccggtc
    31021 gcgtaactca aaatggcgcg gtctggcgga tccagcttgg cccattcgcc agcaaagccg
    31081 aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca attacagtca tttattacta
    31141 ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt ctgtaaatga cgttaacaaa
    31201 gtcacgcgga aagtcagatg cctgccggta gtgcatttgc tatagtaggg cactttttta
    31261 attccatcac ggatgtcgta gttcagacca tgaataccat tttttccgct cgtatcatga
    31321 agcgcctggc gctcaccacg gctctttgca cagcctttat ctctgctgca catgccgatg
    31381 acctgaatat caaaactatg atcccgggtg taccgcagat cgatgcggag tcctacatcc
    31441 tgattgacta taactccggc aaagtgctcg ccgaacagaa cgcagatgtc cgccgcgatc
    31501 ctgccagcct gaccaaaatg atgaccagtt acgttatcgg ccaggcaatg aaagccggta
    31561 aatttaaaga aactgattta gtcactatcg gcaacgacgc atgggccacc ggtaacccgg
    31621 tgtttaaagg ttcttcgctg atgttcctca aaccgggcat gcaggttccg gtttctcagc
    31681 tgatccgcgg tattaacctg caatcgggta acgatgcttg tgtcgccatg gccgattttg
    31741 ccgctggtag ccaggacgct tttgttggct tgatgaacag ctacgttaac gcactggacc
    31801 tgaaaaatac ccacttccag acggtacatg gtctggatgc tgatggtcag tacagctccg
    31861 cgcgagatat ggcgctgatc ggccaggcat tgatccgtga cgtaccgaat gaatactcga
    31921 tctataaaga aaaagaattt acgtttaacg gtattcgcca gctgaaccgt aacggcctgt
    31981 tatgggataa cagcctgaat gtcgacggca tcaaaaccgg acacactgac aaagcaggtt
    32041 acaaccttgt tgcttctgcg actgaaggcc agatgcgctt gatttctgcg gtgatgggtg
    32101 gacgtacttt taaaggccgt gaagccgaaa gtaaaaaact gctaacctgg ggcttccgtt
    32161 tctttgaaac cgttaaccca ctgaaagtag gtaaagagtt cgcctctgaa ccggtttggt
    32221 ttggtgattc tgatcgcgct tcgttagggg ttgataaaga cgtgtacctg accattccgc
    32281 gtggtcgcat gaaagatctg aaagccagct atgtgctgaa cagcagtgaa ttgcatgcgc
    32341 cgctgcaaaa gaatcaggtc gtcggaacta tcaacttcca gcttgatggc aaaacgatcg
    32401 agcaacgccc gctggttgtg ttgcaagaaa tcccggaagg taacttcttc ggcaaaatca
    32461 ttgattacat taaattaatg ttccatcact ggtttggtta aaaattgaac acttgaaagt
    32521 gtaatttccg tccccatata ctaagcatca gtaaaaaaac tcccgccttc tggcgggagt
    32581 tgctatttaa ttacgttacg ccggagctga catgaaaacc aaacttaacg aactgcttga
    32641 attccctact ccttttactt acaaagttat ggggcaggcg ttacctgagc tggttgatca
    32701 ggtggttgaa gtggtacagc gccatgcgcc aggtgactac accccaacgg taaaaccaag
    32761 cagcaaaggc aactaccact cggtatctat cactatcaac gccactcata tcgagcaggt
    32821 tgaaacactg tatgaagaac tgggcaaaat cgatattgtc cgcatggttc tgtaactcgc
    32881 ttctccgtta cccggctcct ggtcgggtaa ctcccctctc ctcgctgtga tatacttttc
    32941 ccccactttt actcattctc cacggagatg ccgttttgta tcaggataaa attcttgtcc
    33001 gccagctcgg tcttcagcct tacgagccaa tctcccgggc tatgcatgaa ttcaccgata
    33061 cccgcgatga tagtaccctt gatgaaatct ggctggtcga gcactatccg gtattcaccc
    33121 aaggtcaggc aggaaaagcg gagcacattt taatgccggg tgatattccg gtgatccaga
    33181 gcgatcgcgg tgggcaggtg acttatcacg ggccgggaca acaggtgatg tatgtgttgc
    33241 ttaacctgaa acgccgtaaa ctcggtgtgc gtgaactggt gaccttgctt gagcaaacag
    33301 tggtgaatac cctggctgaa ctgggtatag aagcgcatcc tcgggctgac gcgcctggtg
    33361 tctatgtcgg ggaaaagaaa atttgctcac tgggtttacg aattcgacgc ggttgttcat
    33421 tccacggtct ggcattaaac gtcaatatgg atctttcacc atttttacgt attaatcctt
    33481 gtgggtatgc cggaatggaa atggctaaaa tatcacaatg gaaacccgaa gcgacgacta
    33541 ataatattgc tccacgttta ctggaaaata ttttagcgct actaaacaat ccggacttcg
    33601 aatatattac cgcttaattc catacatcaa tggcctaatt tacactgggt cattactcat
    33661 aatacctgct aatgctttac atctccttcg cgtttgtata ttttctttat cacttgcaga
    33721 aatgcggtgc cacgccttgc tccgtaactc accccattga ttaacctatt gaaatagttg
    33781 attatggtga gcaaaagcgt tccgttccaa ttagacataa gattattgta attttttaaa
    33841 tattattttt ccatgaataa aattggagta agtgcgtgga tagtaataat caaattgaac
    33901 cctgtttaag cagaaagtcg tcagaaggta aaccacaaat atttacaacc ttacgaaata
    33961 ttgatcttaa ccttctgact atttttgaag ctgtatatgt acataaaggg atcgttaatg
    34021 cagcgaaagt gcttaatctg accccctcgg caatcagtca gtctattcag aaactgcgcg
    34081 ttatattccc taacccattg tttattcgca aaggccaggg tgtcactcct accgcatttg
    34141 cgatgcatct acatgagtat atcagtcagg gccttgagtc cattcttggc gcgctggata
    34201 tcgaaggaag ctatgataag caacgaacga taactattgc gaccactccc tcggtcggag
    34261 ccctggtcct tcctgtcatc tatcgggcga ttaaaactca ctatccgcag cttttactgc
    34321 gcaacccgcc catcagcgac gcggaaaacc aactcagtca gtttcaaacc gatctcatca
    34381 tcgataacat gttttgcacc aatcgtacgg tgcaacatca tgttctgttc accgacaata
    34441 tggtgttaat ttgccgcgag ggaaatccac tactctcttt agaagatgac agagagacta
    34501 tcgacaacgc tgcgcatgta cttctgttac cggaagggca aaatttcagc ggtctgcggc
    34561 agagagttca agagatgttt ccggaccggc aaatcaattt caccagctac aacattttga
    34621 caatcgctgc actggttgcc aacagtgaca tgttagcgat tattcccagc cgtttttata
    34681 acctgtttag tcgttgctgg ccgctggaaa aactgccttt tccgtcctta aatgaggagc
    34741 aaatagattt ctctatccac tacaacaaat tcagcctgcg tgatcccatc ctgcacggag
    34801 taatagatgt tatccgtaat gcattttgaa aaagtattgt tacagccaga tcacagcgca
    34861 gaacgataaa atggcacaat ccaacaacaa ttttacattt tcgcgaccgc tttggctgct
    34921 ttcaggtccg tttcaatgat atactgccag tcgttaattc aaaaatagtt gataattaca
    34981 acaatctatt gaattgaaac gctttccttc gtaattcgca actggaacac gcacgctatg
    35041 agtaaaccca ttgtgatgga acgcggtgtt aaataccgcg atgccgataa gatggccctt
    35101 atcccggtta aaaacgtggc aacagagcgc gaagccctgc tgcgcaagcc ggaatggatg
    35161 aaaatcaagc ttccagcgga ctctacacgt atccagggca tcaaagccgc aatgcgcaaa
    35221 aatggcctgc attctgtctg cgaggaagcc tcctgcccta acctggcgga atgcttcaac
    35281 cacggcacag caacgtttat gatcctcggc gctatttgta cccgccgttg tccgttctgt
    35341 gatgttgccc acggtcgccc ggtagctcct gatgccaatg aaccagtgaa actggcgcag
    35401 accattgccg atatggcgct gcgttatgtg gttatcacct ccgttgaccg tgatgacctg
    35461 cgcgatggcg gtgcccaaca ctttgcggat tgtattactg ccattcggga aaaaagcccg
    35521 caaatcaaaa ttgaaactct ggtgccggat ttccgcggtc gaatggatcg tgctctggat
    35581 attctgactg caacgccccc agatgtgttc aaccataacc tggaaaacgt accgcgtatt
    35641 taccggcagg tacggcctgg cgcagattac aactggtcgc tgaagctgct ggaacgcttt
    35701 aaagaagcgc atccggaaat cccgaccaag tctggtctga tggtgggtct gggtgaaacc
    35761 aatgaagaaa ttattgaggt aatgcgcgac ctgcgccgtc atggtgtgac gatgttaacg
    35821 ctggggcaat atttgcagcc aagccgccat cacctgccgg ttcaacgtta cgttagcccg
    35881 gatgagttcg acgaaatgaa agccgaagcg ctggcgatgg gctttaccca tgctgcatgc
    35941 ggtccgtttg tccgctcttc ttaccacgcc gatttgcagg cgaaagggat ggaagttaag
    36001 taatcctgta ataattactt acatctgtct gataaaaaac ccgctttttg aagcgggttt
    36061 tttgtatcga acaagatatt gagggagcgt cctgctcgcc acgtcactct ttatgagaga
    36121 gcttttcagc ctgaagatca acgtctgcgc cttttttcgc cgcagcatcg tcatcattca
    36181 tcgccttctt gaacccttta atggccgctc caaggtctcc gcccagcgta cgtaacttct
    36241 tagtcccaaa cagcagaacg accagcgccg caactaccag cagtttggta atactaatct
    36301 cacccataga taccttcttg acatataacc ggcaacagaa cgcgccttaa aaaccagaga
    36361 atattaacgg tcattcgacg cgcgcacaca atagcaatct gtaacaaggt gaatcaagcg
    36421 ccgagtaaaa aaacatcata ataattgcgg cggcgcaaag cgacggttgt ttaagatggg
    36481 cagttgcgcg cgcacctgac gcacacgttc gggcgtcact tccgccataa tgagtgcagg
    36541 catttctgac gctgccgcaa tggtgacgcc aaagggatct ataatccggc tttgcccgat
    36601 atttttgttc ccgcactccc ccgccgccac catataacag gtggtatcca gcgcacgagc
    36661 ggcaagcaac gttgaccagt gatgctcttt gagcggccca cgaacccagg cggcaggaag
    36721 taccaggatt tcagctccct gtaaagcctg tgccagcgcc agctctggaa agcgtaagtc
    36781 ataacaggtc atcagaccga ccttcatccc ttccacctcc agtaacggag ctatttcatt
    36841 accagcatca acatggcgtg attcctgaat ggcaaatgca tcatagagat gcagtttggc
    36901 ataacgggcg acgatgtttc ctgcctgaag tgccaccagc atattccatg cgcgccccgg
    36961 cgttgaagga agatgaatcg tcagaattgt cgtcatcatg ttacgtttac tttctcgccg
    37021 taaacgtccg aggaattcgc cttccagcag ctgtgctgat ttaaccgata gatctgcatc
    37081 atgatcatcg cgcgccagca atgcttccgg caggacaaac agcgatacgt cgttttccgc
    37141 cgcctgcgcc atcaacgagg cacaaatctc agcgttcttt tcccacacag atgtaacagc
    37201 aaactgtcct gcagcaacca gcattgccac ctcctgtcag agtcgtaaaa ttcgccaacg
    37261 acgattattc ggcgttacac ttatcactca tacaaatcaa atagcaggat tttgcagtgt
    37321 tacaacttct tttagcagtt tttattggcg gtggtacggg aagcgtagcg agatggctgt
    37381 taagtatgcg atttaacccg ctgcatcagg cgattccgtt ggggacgctg gcagcaaacc
    37441 tgattggggc attcatcata ggaatgggat tcgcctggtt cagcaggatg acgaacattg
    37501 atccagtgtg gaaagtatta atcaccaccg gattttgtgg cggtctaaca accttctcaa
    37561 cattttcggc agaagtggtg tttttgttac aagagggccg ctttggctgg gcattactga
    37621 acgttttcgt caaccttctg gggtcttttg ccatgaccgc actggcattc tggctgtttt
    37681 cggcctcaac cgcacactaa agaccaaaaa aaacccgctg attaagcggg ttttgaattc
    37741 ttgctgacgt atcttacaga gcgattacgt ttgcagcaga agggcctttg gcaccgttag
    37801 tgatttcgaa ctctacgcgc tgaccttcag caagagtttt aaaaccatta gtctggattg
    37861 cagagaagtg tacgaacacg tctttgctgc cgtcttccgg agtaatgaaa ccgaatcctt
    37921 tggactcatt aaaccactta acgttacctt taatcttaga catcaaaatt acctttacat
    37981 gaaaaataga cacaaatgct gtgtcggtta tcagtacacc aattgtggta cgctttgtcc
    38041 agtgcaactt tactaaaaag tgataaaagt cgcgttattt ttgatgttgc ttattttagt
    38101 tattgatttt aaagaagtta ctaaaacttc atttgtctca aaactgaaag cgcatccagg
    38161 caaagtacac attgccattg ttgtaggtac ccggaatgta ggtcatctga aaagtcactg
    38221 ggccataacc cacggaggcc aatggcagta gaaccgggag agggatgtaa ttccagttat
    38281 cgcgtgccgt tacgccagcg gtgaatccca gacctaaatg aaaattttca tccgccagcg
    38341 gtcgccaggt actttcccat ccgtatccgg caatcggttc ccatttgttc cacgagtcct
    38401 taaatgccat ggcatacagg ccatgccagt ttcctttttc atcccaacgc gacaggccaa
    38461 aaccgccacc ccacggtcgc tcgttatagc gatcggtttt ttctttgtcg taagcgaaac
    38521 gtgcatgcca ggtgatggca ggaatatata aatcataatg ttcaggctgt tgccaggttt
    38581 gtgcaatatt ttctctaaac gttgtcatcc tctcatctgc gttagcaaaa actttaccaa
    38641 cgctgattaa ctgaataaaa acaaaggaaa agatagcgac atatttactc acgttcattt
    38701 gtgaccataa aacatttatc aaaaatctac tactagcata gcaaagctac aattaacata
    38761 accttaatag acccaacata aagaataatc tgaatagctg ctctatttat ttccgcttgt
    38821 tcaagttaaa tatttccgcg catcgattaa agatgatgcg tcgttttctg cgatgggaat
    38881 agtcaaaaaa gaaaaaccaa gtcttttttg atgacaaaat gcaatcaagg aaaaattatt
    38941 ttatttttta acgagataca acaatcatct taacgaagta tataatatat atcgtcaacc
    39001 catgctcata ttagattatt ttttgagcgc acagccgcag ctaacaccat tgcaattaac
    39061 aaatttgcat caatcgccat caatttacac acattattat gtgataattg ccaaccgcta
    39121 aatatgcgtt ttgttatcta tgtataaaaa cagcaacttc aatgtcttaa tggcagtttt
    39181 tcttgatttt aatcagcatt catcgccaat ttattgggca tattttttcc ttaagcttta
    39241 ggaatttttt attatttact ttgggcctgg agacaggaaa aattatgctg acattcattg
    39301 aactccttat tggggttgtg gttattgtgg gtgtagctcg ctacatcatt aaagggtatt
    39361 ccgccactgg tgtgttattt gtcggtggcc tgttattgct gattatcagt gccattatgg
    39421 ggcacaatgt gttaccgtcc agccaggctt caacaggcta cagcgccacg gatatcgttg
    39481 aatacgttaa aatattacta atgagccgcg gcggcgacct cggcatgatg attatgatgc
    39541 tgtgtggctt tgccgcttac atgacccata tcggcgcgaa tgatatggtg gtcaagctgg
    39601 cgtcaaaacc attgcagtat attaactccc cttacctgct gatgattgcc gcctattttg
    39661 ttgcttgtct gatgtcactg gccgtctctt ccgcaaccgg tctgggtgtt ttgctgatgg
    39721 caaccctgtt tccggtgatg gtaaacgttg gtatcagtcg tggcgcagca gctgccattt
    39781 gtgcctcccc ggcggcgatt attctcgcac cgacttcagg ggatgtggtg ctggcggcgc
    39841 aggcttccga aatgtcgctg attgacttcg ccttcaaaac gacgctgcct atctcaattg
    39901 ctgcaattat cggcatggcg atcgcccact tcttctggca acgttatctg gataaaaaag
    39961 agcacatctc tcatgaaatg ttagatgtca gtgaaatcac caccactgcc cccgcgtttt
    40021 atgccatttt gccgttcacg ccgatcatcg gtgtactgat ttttgacggt aaatggggtc
    40081 cgcaattaca catcatcact attctggtga tttgtatgct gattgcctcc attctggagt
    40141 tcctccgcag ctttaatacc cagaaagttt tctctggtct ggaagtggct tatcgcggga
    40201 tggcagatgc gtttgctaac gtggtgatgc tgctggttgc cgctggggta ttcgctcagg
    40261 ggcttagcac catcggcttt attcaaagtc tgatttctat cgctacctcg tttggttcgg
    40321 cgagtatcat cctgatgctg gtattggtga ttctgaccat gctggcggca gtcacgaccg
    40381 gttcaggcaa tgcgccgttt tatgcgtttg ttgagatgat cccgaaactg gcgcactctt
    40441 ccggcattaa cccggcgtat ttgactatcc cgatgctgca ggcgtcaaac ctgggtcgta
    40501 ccctatcacc cgtttctggc gtagtcgttg cggttgccgg gatggcgaaa atctcaccat
    40561 ttgaagtcgt aaaacgcacc tcggtgccgg tgcttgttgg gctagtgatt gttatcgttg
    40621 ctacagagct gatggtgcca ggaacggcag cagcggtcac aggcaagtaa atagtaatgc
    40681 cggaggcaag tttcctccgg ctttatcatg tcacccactg tggtatatgc gttgtggtct
    40741 gccaactttg ccgtgaacaa tttcagcaat aatcagatgg cggctggcgc aatattcaag
    40801 ataacgcctg gcagtggtgc ggctgatggt cagtgcctgc gccaccgttt ctgccgtatg
    40861 ttgcacacaa ggctctttaa acagttttcg caccgcgttt agcgtcaagg ggtcaatgcc
    40921 ggtcggcagt tcgtctttgg gttcaccgcg agcataggca ttaaacatct catcaatttg
    40981 cttctggctg gcgctatcaa tactttccag catatgttta cgctggcgga aacgggttag
    41041 cgtttgcccc agtgtattga ttcacttgaa gtacgaaaaa accgggagaa cattggatta
    41101 ttcgggatct gatgggatta gatttggtgg ggcttgcaag cctgtagtac aaattttagg
    41161 cgttaaccaa tgaaacgcga aagatagtaa aaaattgctt ttgtttcatt gaaaaaacga
    41221 aaaacaaaaa cactataaat c
//
LOCUS       DADLSA010000019        40654 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.019, whole genome shotgun sequence.
ACCESSION   DADLSA010000019 DADLSA010000000
VERSION     DADLSA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.019"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(163..954)
                     /gene="cpoB"
                     /locus_tag="JN691_001047"
     CDS             complement(163..954)
                     /gene="cpoB"
                     /locus_tag="JN691_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAY7242547.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNATIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(964..1485)
                     /gene="pal"
                     /locus_tag="JN691_001048"
     CDS             complement(964..1485)
                     /gene="pal"
                     /locus_tag="JN691_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAY7242548.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1520..2812)
                     /gene="tolB"
                     /locus_tag="JN691_001049"
     CDS             complement(1520..2812)
                     /gene="tolB"
                     /locus_tag="JN691_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAY7242549.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGLGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(2945..4186)
                     /gene="tolA"
                     /locus_tag="JN691_001050"
     CDS             complement(2945..4186)
                     /gene="tolA"
                     /locus_tag="JN691_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAY7242550.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4251..4679)
                     /gene="tolR"
                     /locus_tag="JN691_001051"
     CDS             complement(4251..4679)
                     /gene="tolR"
                     /locus_tag="JN691_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAY7242551.1"
                     /translation="MARARGRGCRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4683..5375)
                     /gene="tolQ"
                     /locus_tag="JN691_001052"
     CDS             complement(4683..5375)
                     /gene="tolQ"
                     /locus_tag="JN691_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAY7242552.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5372..5776)
                     /gene="ybgC"
                     /locus_tag="JN691_001053"
     CDS             complement(5372..5776)
                     /gene="ybgC"
                     /locus_tag="JN691_001053"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY7242553.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(5926..6219)
                     /gene="ybgE"
                     /locus_tag="JN691_001054"
     CDS             complement(5926..6219)
                     /gene="ybgE"
                     /locus_tag="JN691_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAY7242554.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6219..6332)
                     /gene="cydX"
                     /locus_tag="JN691_001055"
     CDS             complement(6219..6332)
                     /gene="cydX"
                     /locus_tag="JN691_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAY7242555.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6347..7486)
                     /gene="cydB"
                     /locus_tag="JN691_001056"
     CDS             complement(6347..7486)
                     /gene="cydB"
                     /locus_tag="JN691_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY7242556.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7502..9070)
                     /gene="cydA"
                     /locus_tag="JN691_001057"
     CDS             complement(7502..9070)
                     /gene="cydA"
                     /locus_tag="JN691_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAY7242557.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(9919..12552)
                     /gene="mngB"
                     /locus_tag="JN691_001058"
     CDS             complement(9919..12552)
                     /gene="mngB"
                     /locus_tag="JN691_001058"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HAY7242558.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKSENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIDSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(12570..14547)
                     /gene="mngA"
                     /locus_tag="JN691_001059"
                     /pseudo
     CDS             complement(12570..14547)
                     /gene="mngA"
                     /locus_tag="JN691_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706503.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
     gene            14657..15379
                     /gene="mngR"
                     /locus_tag="JN691_001060"
     CDS             14657..15379
                     /gene="mngR"
                     /locus_tag="JN691_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAY7242559.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15483..16352)
                     /gene="sucD"
                     /locus_tag="JN691_001061"
     CDS             complement(15483..16352)
                     /gene="sucD"
                     /locus_tag="JN691_001061"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAY7242560.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPEGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16352..17518)
                     /gene="sucC"
                     /locus_tag="JN691_001062"
     CDS             complement(16352..17518)
                     /gene="sucC"
                     /locus_tag="JN691_001062"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAY7242561.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMCDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17612..18829)
                     /gene="odhB"
                     /locus_tag="JN691_001063"
     CDS             complement(17612..18829)
                     /gene="odhB"
                     /locus_tag="JN691_001063"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAY7242562.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(18844..21645)
                     /gene="sucA"
                     /locus_tag="JN691_001064"
     CDS             complement(18844..21645)
                     /gene="sucA"
                     /locus_tag="JN691_001064"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAY7242563.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVVTLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSCLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(21946..22662)
                     /locus_tag="JN691_001065"
     CDS             complement(21946..22662)
                     /locus_tag="JN691_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAY7242564.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22678..24444)
                     /gene="sdhA"
                     /locus_tag="JN691_001066"
     CDS             complement(22678..24444)
                     /gene="sdhA"
                     /locus_tag="JN691_001066"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAY7242565.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24444..24791)
                     /gene="sdhD"
                     /locus_tag="JN691_001067"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24444..24791)
                     /gene="sdhD"
                     /locus_tag="JN691_001067"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAY7242566.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(24785..25189)
                     /gene="sdhC"
                     /locus_tag="JN691_001068"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(24785..25189)
                     /gene="sdhC"
                     /locus_tag="JN691_001068"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY7242567.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25570..25677
                     /locus_tag="JN691_001069"
     CDS             25570..25677
                     /locus_tag="JN691_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242568.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            25883..27166
                     /gene="gltA"
                     /locus_tag="JN691_001070"
     CDS             25883..27166
                     /gene="gltA"
                     /locus_tag="JN691_001070"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAY7242569.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27556..28122
                     /locus_tag="JN691_001071"
     CDS             27556..28122
                     /locus_tag="JN691_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7242570.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28173..30524
                     /locus_tag="JN691_001072"
     CDS             28173..30524
                     /locus_tag="JN691_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7242571.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNFNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAEDVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEG
                     HAWLSGVAENQKVTVVWGDSQHCSLHLPEHMEDTANRLILPCH"
     gene            30539..31270
                     /locus_tag="JN691_001073"
     CDS             30539..31270
                     /locus_tag="JN691_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7242572.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVETKK"
     gene            31267..32328
                     /locus_tag="JN691_001074"
     CDS             31267..32328
                     /locus_tag="JN691_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7242573.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            32435..33526
                     /locus_tag="JN691_001075"
     CDS             32435..33526
                     /locus_tag="JN691_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY7242574.1"
                     /translation="MLYRYTKEKIMRERRMPVLQWGMLCVLSLLLSIGFLAVHLPAAL
                     LLGPMIAGIIFSMRGITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAI
                     LLVTLLSSAIVGWLLVRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYL
                     RVLFVAGAAVLVTRMMLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLP
                     SGTMLIPMLAGAVLQSGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQ
                     ILLSIFALLAICAGMAWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMA
                     MQTLRLFSILLTGPAIARFISTYAPKRSA"
     gene            complement(33523..34314)
                     /gene="nei"
                     /locus_tag="JN691_001076"
     CDS             complement(33523..34314)
                     /gene="nei"
                     /locus_tag="JN691_001076"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAY7242575.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34350..35084)
                     /gene="pxpA"
                     /locus_tag="JN691_001077"
     CDS             complement(34350..35084)
                     /gene="pxpA"
                     /locus_tag="JN691_001077"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAY7242576.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35074..36006)
                     /gene="pxpC"
                     /locus_tag="JN691_001078"
     CDS             complement(35074..36006)
                     /gene="pxpC"
                     /locus_tag="JN691_001078"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HAY7242577.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLFHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(36000..36656)
                     /gene="pxpB"
                     /locus_tag="JN691_001079"
     CDS             complement(36000..36656)
                     /gene="pxpB"
                     /locus_tag="JN691_001079"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAY7242578.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDSARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(36679..37422)
                     /gene="ybgI"
                     /locus_tag="JN691_001080"
     CDS             complement(36679..37422)
                     /gene="ybgI"
                     /locus_tag="JN691_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAY7242579.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            37693..39174
                     /gene="dtpD"
                     /locus_tag="JN691_001081"
     CDS             37693..39174
                     /gene="dtpD"
                     /locus_tag="JN691_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAY7242580.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPDVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39324..>40654)
                     /gene="phrB"
                     /locus_tag="JN691_001082"
     CDS             complement(39324..>40654)
                     /gene="phrB"
                     /locus_tag="JN691_001082"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAY7242581.1"
                     /translation="VLALYIATPRQWETHNMSPRQAELINAQLNGLQIALAEKGIPLL
                     FREVDDFVASVEIVKQVCAENSVTHLFYNYQYEVNERARDVDVERALRNVVCEGFDDS
                     VILPPGAVMTGNHEMYKVFTPFKNAWLKRLREGMPECVAAPKVRSSGSIKPAPSITLN
                     YPRQSFDTAHFPVEEKAAIAQLRQFCENGAGEYEQQRDFPAVEGTSRLSASLATGGLS
                     PRQCLHRLLAEQPQALDGGAGSVWLSELIWREFYRHLMTYYPSLCKHRPFIAWTDRVQ
                     WQSNPAHLQAWQKGKTGYPIIDAAMRQLNSTGWMHNRLRMITASFLVKDLLIDWREGE
                     RYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIFNPITQGEKFDREGEFIRRWLPE
                     LRDVPGKAVHEPWKWAQKAGVKLDYPQPIVEHKEARIQTLAAYEAARKGK"
BASE COUNT         9820 a        11003 c         9853 g         9978 t
ORIGIN      
        1 tttttcgttg ctttcggttt gtttgatatc ccgtggcaac ggcggcatat attactgatt
       61 tcagacttga gcgcaacaaa aatttcgatg cagatcactc aactgcttat gattcgcacg
      121 acacgaccag aaataatgcg acttctggtc gtgtgttatg cattacatcg cgttcagacg
      181 tttttgtgcc tgtttagcgc catcagtacc agggtattta ctgataacct gctggtatac
      241 ggctttcgct tttgcggtgt cacctttgtc ctgcatgatg acgccgactt taaacatcgc
      301 atcagcagcc tttggtgact tcggatagtt tttcactacc gaagcaaaat agtacgccgc
      361 atcatctttt ttacccttgt tgtagtttaa ctgacccagc caataattgg cgtttggcag
      421 gtaagttgaa tcagggtaat ttttgatgaa attctgaaat gccaccattg cgtcatcctg
      481 gcgggattta tcctgcacca gcgcaatagt tgcattgtaa tccgtgtttg catcgccgct
      541 tttcaccggc gcgccagcat tcgcagtacc agcatcagct gtcggcgtcg ttgatgccgc
      601 cgcaccgctt tgatcgctgc tggttgattg cgccgctgca ccaccgctgc tgaggctgtc
      661 gatctgcaac aggatctgct tttgccgctc cacgacctga ttcagttgat actgattttc
      721 ctgaatctga ccacgcaggg aatcaatatc ggattgatta tcagaaagtt gttgctggag
      781 ttgggttaaa agctggctgt gagcattaga aatacgctca agttgagtga cgcggtcttc
      841 gaccgagcct gagccgacac tactgattgg tgcctgagca aaagcggccc agggggccgc
      901 tataccaacc agtaacgaca gactcaatag ttgatgtctg aagttactgc tcatgcaatt
      961 ctcttagtaa accagtaccg cacgacggtt tttggagtat gccgcttcgt catgacccag
     1021 tactgcaggt ttttctttac cgtaagaaac gatggagatc tggtctgcag aaacgccttt
     1081 accctgcagg tacatcttaa cggcgttcgc acgacgttca cccagggaga tgttgtattc
     1141 cggagtacca cgttcgtccg cgtgaccttc tacggtgact ttgtaagacg ggttgctacg
     1201 caggaagttt gcatgtgcat ccagcatttg agcgaagtca gaacggatat cgtacttgtc
     1261 cagatcgaag taaacgatgt tgttctgctg cagctgttgc atttgcagac gagcctgctc
     1321 ttcggaagac atgttgccgt tgccgccgtt cgcatccata ccagtgccgg cacccagcat
     1381 gccttcgctg ccgtcattgc tggcgttctt gttggaagaa catgccgcaa ttgccataac
     1441 aggcagagca atcatcagcc ctttcagcac tttgttcagt tgcatttcaa tgattccttt
     1501 actattcaat taattattat cacagatacg gcgaccaggc agggaatttg acctgtccat
     1561 cagttgccgg aagacgcgct ttgaaacgcc catctgtaga aaccaaattc agcacggatc
     1621 ccatcccctg agaagagctg tagattacca tagtgccgtt aggtgccaga cttggcgttt
     1681 catctaggaa cgtggacgac agaacttgta cgcctcccgt tgccagatct tgtttggcaa
     1741 tgtgctgctg cccaccattg gagctgacca ttaccataaa tttaccgtcg ctgctgacat
     1801 ccgcatcctg gttctgcgaa ccttcccagg taatacgttg tggcgcaccg ccgttgatat
     1861 tcactttata aacctgcgga cgaccggcct ggtcagaagt aaatgccagg ttctggctat
     1921 ccgggaacca ggtcggttcg gtattgttac tgcgaccatc agtcacctgg cggatctgac
     1981 cagaagccaa atccattacg tacaggttca gactaccggt tttcgacaag gcgaatgcca
     2041 gtttgctgcc gtctggcgag aatgcaggtg caccgttgtg acgcgggaat gaagccacct
     2101 gacgtacagc gccatttgcc agcgtctgaa taaccagcgc ggaacgaccg ctttcgaagg
     2161 tcacataagc cagtttagaa ccgtctggtg accacgccgg agacatcagc ggctgcggtg
     2221 aacggtgaac gacaaactgg ttgtaaccgt catagtcaga tacgcgcagt tcatacggga
     2281 actgaccgcc gttggtctga acaacgtagg caatacgggt acggaacgca cctttaatgc
     2341 cggtcagttt ttcaaacact tcatcactgg cggtatgacc agcataacgc agccactgct
     2401 tgttcacttt gtacgagttc tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa
     2461 gttgataagc aacattgtaa gaaccatccg gattcggagt gacctgaccg acaactacag
     2521 cgtcaatgcc cagtgcggac catgcagctg gttgtacttc ctgcgcacta cccggctgct
     2581 gtggcagacg agcgcgatct aacggattaa atttaccgct gttacgcaag tcagcagcaa
     2641 cgatgccgcc aatatcttca ggtgccgcac caagccccgc ccactggaaa ggaacaacac
     2701 caataggacg accggaatct acaccgctgt cgatcacaat gcggacttca gcatgcagaa
     2761 ctgatgccca cagtatgaga aaaccaaatg ctactcgtaa tgcctgcttc atcatatctc
     2821 ccttatctgg accgatggcc cacgataatt tagcagaatg ttaacaaact aaaatacaca
     2881 aaactaccag aaccccgtgg caaccggtgc ctgatgttga ccgtccgaac agtcaacatc
     2941 gcgattacgg tttgaagtcc aatggcgcgt ttttgaacac ttcatatact gcctggcttg
     3001 gtggtttcgg gatcttagca agtttagctg ctgccaacgc agcctgacaa agtgcgggat
     3061 cgccaccttc aggtttgata tccagtaaca taccatcggg tgccagtttt atgcgcagcg
     3121 tacaggtttt gcctgcatag gacgatgcgt catagaactt actttcgata gcagatttaa
     3181 tctgcccggc atagttattg atatcggccc ctgatgcgcc attgttttta gtattaccac
     3241 tcccggcagg cgaagcattg ttccctttcg cccctccccc cgttttcggt gcattcttac
     3301 cagagcttag ctcaccgaaa atatcatctg cctctgcggc agcttttgct gcagcggctt
     3361 tttcagctgc tgcttttgcc gctgccgctt ttttgtctgc ggcggctttt tctgccgctg
     3421 ctttcttatc agctgctgcc ttatcagcag ccgctttttc tgctgctttt ttgtcggctg
     3481 cagctttctc tgctgccgct ttcttatcag ctgcagcctt ttcagcagcc gctttcttct
     3541 cagcttctgc cttggctttt tcagcagctt cagttgccgc tttctttctt gcttcagctg
     3601 cagctgcttc tgccgcttcc gctttcttct tcagtgccgc agcggctacc tcggcttttt
     3661 tctgcgcttc ggctgcggct ttggcggctt ctgcttctgc ttttttcttc gcgtctgcag
     3721 ccgctttctt cgctgcttct tccgcagctt tagcatctgc cgccgctttc gctgccgcca
     3781 cttcagcttg cttctgcttt aactcggcct gttttgcggc ttcttcagcc tgctttttct
     3841 gctcctgagc cgctaaccgc tctttctcaa gttgcttcag gcgttcctgt tcagccgctt
     3901 gtttctcacg cagttcttca gcagcctgct gttccttcat cttgcgctgt tcatcagaac
     3961 gcttcgcgct tgattcctgg ctttgcatgc gtttgtactg ctcaactacc gcacctgaat
     4021 caaccatgac agcgtcgatg gacgaaccac cgccgcctcc ggctgaagct tctatattct
     4081 catcgaacga actccagatc agcgccgcaa ataagatgac atgcagcact gctgaaataa
     4141 ttatcgcccg cttgagcttg tcgttttgtt cggttgcctt tgacactctc ggtttccaaa
     4201 aactgttcgc ctgttacccg ctctctttca agcaagggaa acgcagatgt ttagataggc
     4261 tgcgtcatta aaccaaccga tttcacaccc gcactatgta acaagttcag tgctttaatt
     4321 atttcatcgt aaggcacatc ttttgcgcca ccgatcagaa agaccgtttt cgggttggcc
     4381 ttgaaacggc tggacacttc cgccaccacc tgctctggtg gtaaacgctc taggcgatct
     4441 ttctcaacca ccacggtgta ctgaccaata ccagacactt caacaatcac tggcggatta
     4501 tcgttactgc tcaccgcctg tgattcagta gcgtctggca gatcgacctc cacgctctgg
     4561 gtgatgatgg gcgctgtcgc cataaagatc agcaacagca ccagcagtac gtccagcaac
     4621 ggtacaatgt tgatttcgga cttgagatcg cgacaacctc gtccacgcgc tctggccatg
     4681 gcttacccct tgttgctctc gctaacggta aacgcctggc ggtgcagaat cgcggtaaac
     4741 tcttccataa agttgtcgta attcagttcc agtttgttta cgcgctggtt gagacggtta
     4801 taagccatta ccgccggaat tgcggcgaac agaccaattg ccgtcgcaat caacgcttct
     4861 gcgataccgg gcgcaaccat ttgcagtgtt gcttgtttta ccgccccgag ggcgataaag
     4921 gcgtgcatga tcccccagac cgtaccaaac agaccaatat acgggctgat ggagccaacc
     4981 gtaccgagga acggaatgtg cgtttccaga ttttcaagtt cacggttcat ggagatacgc
     5041 atagcacgcg acgccccttc cactacggct tccggcgcat ggctattggc acgatgcagg
     5101 cgcacaaact ctttgaaccc gctgtaaaag atttgttccg aaccagtcag attatcccgt
     5161 ttcccctgac tctcttgata gaggcgagag agttcgattc cagaccagaa tttatcttca
     5221 aacgcttcgg cttcacgcgc cgctgcgtta agaatacggg ttcgctggat aataatggcc
     5281 caggatgcga ttgaaaaacc aatcaaaatc aacatgataa gtttaaccag aaggctagcc
     5341 ttcaggaaca aatcaaggat attcatgtca gtcactgctt aaactccgcg acaatagact
     5401 tgggaagcgc acgaggcttc attttgagtg ggtcaacgca aacaaccaga acctctgctt
     5461 cattcagcaa agtattctcg gcgttgacaa tacgttgcgt gaaaaccaaa gaggtgccac
     5521 gcattgatgt tatttcagtc tgtatttcga gcatatcgtc gagccgcgca ggtgcgtaat
     5581 attccaccgt cattttacgt accacaaagg caacgcgttc agccatcagc gcctgctgac
     5641 tgaagtgatg atgacgcagc atctctgtgc gtgctctttc ataaaaagcg acgtaactgg
     5701 cgtggtacac cacaccaccg gcatcggtat cttcatagta gacgcgaacc ggccatcgaa
     5761 acagcgttgt attcacttta catcccggta atgcaacaaa agttagagct tttaaacgct
     5821 gctactatac gcgcgcgatt gaggtttggg aatgagggca agttaaggga gcgtaaaaat
     5881 ttatgggcct ggagcggccc ataatttttt aaagagaatt ctgatttaaa agaagaagaa
     5941 aatcagcccg acaatgagaa caatatcggc aagcaatggg caaaaaatcc cttgccaaag
     6001 aaccttctgc ggacgaaagc ccacgccgtg aatcacacca gcacagacgg cccacatcag
     6061 caataaacca tgccagattt ccagttcact ggtcttcgcg gcaaaacgag atgggtccca
     6121 aaacatacat cctgctaaca gaagcgccat cacgaaggaa agcgcccgca gggggcgctt
     6181 gtccattacc gcatacaagg tcgcgataat cttactcatc agatgtcttc ttgaccggct
     6241 ttgcctgatt cgacgtgttc aagcgccagc gcggtgatta ccccaaacga acaggcaaga
     6301 agcgttccca gaatccatgc gaaataccac atttttagct ccttacttag tacagagagt
     6361 gggtgttacg ttcaatatct tctttggtga tacgaccgaa cattttccag taacaccagg
     6421 cggtgtagag cagaatgatc ggtaccagaa ccaccgcaac ccaggtcatg acgttaagcg
     6481 tcagctggct ggaagttgca tcccacattg tcagacttgc gttcatcatg gtgctggacg
     6541 gcatcacaaa cgggaacatt gcgataccgg ctgtcaggat gatgcaggcc agcgtcaggg
     6601 aggagaacac aaacgcccac gcggctttat ccatacgtgc agtcaggatg gtcagcagcg
     6661 gcagaaccac acccagtgcc ggaatagccc acagaattgg cgtgttgttg aagttaacca
     6721 gccatgcgcc agcttcacga accacttctt tattcagtgg gttagaggct gcgtaatggt
     6781 ccattgtcga tttcacgaca taaccatcga taccgtacat cacccatacg ccagccagtg
     6841 cgaaacagac cagtgtcacc agcgcagcca cctgagccgt tgcacgggta cgcaggtgca
     6901 gttcgcccac ggtacgcatt tgcagatagg ttgcgccctg agtaatgatc atccctacgc
     6961 tcaccacgcc tgccagcagg ccgaacgggt taagcaactg gaagaagtta ccggtgtagt
     7021 acagacgcag atattcatca acgttgaacg gtacgccctg caacaggtta ccgaacgcta
     7081 caccaattac cagcggcgga acgaagctac caatgaagat gccccagtcc cacatgttac
     7141 gccagcgggt ttcttcaatc ttggagcggt agtcaaaacc gaccggacgg aagaacaaag
     7201 acgccagcac gaggatcatc gccacataga agccggagaa cgcagcggca tagaccatcg
     7261 gccaggcagc aaagagtgcg ccgcccgcgg tgatcagcca aacctggtta ccgtcccagt
     7321 gtggtgcaat ggagttaatc ataattcgac gctcggtgtc gttacgaccg aggaaacggg
     7381 tgagcatgcc cacccccatg tcgaaaccgt cagtgactgc aaaaccaatc agcagaacgc
     7441 caaccagcag ccaccagata aaacgcaata cttcataatc gatcatttga cgactcctgt
     7501 cttagcgtgc cggctgagta gtcgtggaag actgctcaaa gtgatagcga ccggttttca
     7561 ggctgcttgg gccgaggcgt gcaaacttga acattaagaa caattctgcc accaggaaca
     7621 gggtatacag gccgcaaatc agcaccattg agaagatgag atcgcctgcg gtcagtgacg
     7681 aattcgccac agctgtcggc agcacttcac cgatagccca cggttggcgg ccatattcag
     7741 ccacgaacca gcccgcttct acagcaatcc acggcagcgg aataccgtac agcgcggcgc
     7801 gcagaagcca ttttttctcg ccaatgcggt tgcggatgac actccagaaa gagagcgcga
     7861 tgattgccag cagcaggaag ccacacgcca ccatgatacg gaacgcaaag tacagcggcg
     7921 ctacacgcgg gatggagtct ttggttgcct gttgaatctg cgcttcagtc gcatcagcca
     7981 cgtttggcgt atagcgtttc agcagcagac cgtaaccgag gtctttcttc atgctattga
     8041 actggtcacg aaccgcctgg tcggtagaac cagaacgcag ttgttcgagc agagagtacg
     8101 ccttcatccc gttacgaatg cgttcttcat gctgcaccat cagctctttc aggccgataa
     8161 ccggggtatc cacggaacgc gttgcaatga tgcccagtgc gtaagggatc tgaatcgcaa
     8221 atttgttcgt ctcctcttcc tgatcaggaa tgccgaacag agtaaaggca gcaggcgcag
     8281 gttgcgtttc ccactcggct tcaatagcag ccagtttggt tttctgcacg tcgcccattt
     8341 cgtagccgga ttcatcaccc agaacaataa cagacagaac agcagccata ccgaagctgg
     8401 cagcgatagc aaaggagcgt ttagcgaagg cgaagtcacg acctttcagc atataccatg
     8461 cgctgatacc gaggatgaac atcgcgccag tcacataacc agacgctaca gtgtgaacga
     8521 atttcacctg agcaaccggg ttaagcacca gctcggagaa gctcaccatc tccatacgca
     8581 tagtttcaaa gttgaaatcg gacgcgattg ggttttgcat ccagccgttc gcaaccagaa
     8641 tccacagtgc ggacaggttt gaaccgagcg ccaccagcca ggtgacacac atatgctgaa
     8701 ctttacccag acgatcccaa ccgaagaaga acagacctac aaaggtggat tcgaggaaga
     8761 aggccatcag accttcgatt gccagcggcg caccgaagat atcccctaca tagtgggaat
     8821 agtaagacca gttagtcccg aactggaact ccatggtcag accggtagcc acacccagag
     8881 cgaagttgat accaaacaac ttgccccaga acttggtcat atctttataa atctgtttgc
     8941 cggagaggac gtagaccgtt tccataatgg ccaggaggaa cgccataccg agcgtcagtg
     9001 gcacaaaaag gaagtggtac atcgcggtca aggcaaactg taagcgcgac agttcgacta
     9061 tatctaacat catgactcct tgctcatcgc atgaagactc cgagagtgaa ccccgttaga
     9121 aagggtcaca cgcatgcccc aatagaaatt tgtttgaaca ctttcatcat accatgtgaa
     9181 tgcacacaaa aacggcgtaa agaaaggttg caaacatgtt aataaaaact caaattgatc
     9241 ccacgtatat attacgccgc aaaatcctta caataaacag gtttttgttg aagcaaattt
     9301 gcatttttcg acggtgatca atttatagct aaattgccgc ctttcagcca atttgatcga
     9361 gaacaattta tctctttttg atgcccattt ccaagactta tacattgata aatatcaatt
     9421 tctacaaatt atgttaatca tgtttcaata aggtaacttt agtgacattt atgtttaaaa
     9481 tgtgtgaatt ataaggctga aattagtttc ggttggcaat taaagataaa aagagacgaa
     9541 gcaaaaacag cacggttgac cttaattctt tttttggtca ctggctaata cgacgcagag
     9601 tttcaggatt attaataaag gcataaatct cccccccgcc gatattaaca tttaattaac
     9661 ttataattat attataatta actcacccat ggttattatt ttgttaaaaa taaagccaca
     9721 tatacgtggc ttgagagtga agataaacgc aaaattacct gatgcgctac gcttgccagt
     9781 gtctacatca tctctgcaat atattgaatt tgcctgcttt tgtagaccga ataaagcgtt
     9841 catgccgtat ccggcatgaa caaagcgccc tttgtcaaca atctggccgc gcatatgtgg
     9901 cttcggtttg attgctattc aggcaagccg gtaactgaac gtccgtgact ggccgggtaa
     9961 aaaaggcccc gatagatttg aaccttgatt ttcctcggtg gtaatgtgtt catccatcat
    10021 cgtttctgag caagaaatca cctcgcgact gaaagcaaca gtcgcatcac aggttgctga
    10081 ttcagccgga ttaaacagcc gtaaaatcac ttcttgtcgg tcttcagctt tcttaagtgc
    10141 gcttatcagg catcccactg gtggcatttt caacaaacta taactttccg gcacgttgaa
    10201 tccggctttg ttgagcttca tcgcatccca tgggattttg ttgtaacact gtactggagt
    10261 cagccatgct cgcgcctgct gagctacacc agcggcggtt ggcgtaccgg tataactcaa
    10321 taaacttagg cgacaagaaa gcagaccacg tagttgtgag tccgggactg gcattttaat
    10381 tcccgaaggc cgcctaggcc ttaaaagcag atcttctttc cccagtaagc ccacgccgcg
    10441 cagcaacgta atggcaaagg ttttcttctc ttcaccgatg acttcaaatt cacgtagccc
    10501 ttcgctaaag acagccatgc cgttacgccc ttcctgtaag gctgcatagt tgagcatatt
    10561 ccataccgga accggcgctt ctttccagcc ttcttgctgc cagttgttca ttgcactgtc
    10621 gttcacgggg cgcgttagcg aaccaaactg ggtatctgcc agaacactat cggtgttaaa
    10681 agatgtaggg atcaggacgc gaaggcgatg atcgtcagcc tggttatcaa ggttgatatc
    10741 cacatcaatg cgcctgttgt tatgactaag agtgacaacc aactcgacac ctactctgcc
    10801 agtggattgt cttgcgctgc gttgttgcag attacgcggc accgccattt catagcggat
    10861 aacagccctg ctctgccagg cctcatgaat aatctcgcat tgcggtttcg cattcgccga
    10921 agtaattacc cactcttctt ttgcgggtga atagtcatat tcatcaccat catcagagct
    10981 ttcttcaatt tgcaacaccc ggtcatagcg cacaccgcta tctttatcta ccagttgtag
    11041 cgttccatcc tcattgagcg caatttgcca gaaagcattt tccagtatcc cttcagcgtc
    11101 actttttgcc gcaatcacgt tgccaggctg attcgcttcg atataaagcg tgcgatagcc
    11161 catagaaggg acaatctggt tgatctgtat atcaaactcc ataaagggat cgtaattacc
    11221 gtaatgaact atttgccgat cgattaggcc tggatcgatc tcacgggcat ggcgaataaa
    11281 atacggtaca ggctgaccgc gatcgtctcg tagattaaac tggctggcgc gcagccgcac
    11341 agtggtgttg atcacttctt cccgcggcca gggcatcagg ttaaacagga cgagtttgtc
    11401 ggcgtcgctc tgcggcatgt tgtcggcgat tttacgcata tagaaacgca gcagattatc
    11461 cgccatgtct tcagccagtt cgaagcgggc gacgatttcg cgatgaactt tgtcactaca
    11521 gcagcagccg atgctgtcgt gggcatgatt ttttaagatc tctttccaca ttttctccag
    11581 caagccgtgg tggtattcaa aacccaacgt ccaggccagc gttgccagcg gttccagcag
    11641 attaacaatc ttattttcaa tacgtgcgtg ggcaactttg atatccatgc gcgtggaatc
    11701 gatggtgcga tgcacgcgca tatatttgcc atcaataaat tcccctttca gggttgccag
    11761 attatctcgc tgcgcttcga tcttctcaaa tacctcttca aagcggctca tcacaaactt
    11821 tcgttgagga taaatttcgc gcagtttctc catcacttcg aagatatttt gctgcaatgg
    11881 catctgatca tgcccattcg gcagcaaaat ctctttggtt acagacgctt tttccagcac
    11941 gtcaaaataa ctgtcgaggc gtttgcgtaa tccgttttcg tccgcaggta agtacttacc
    12001 gatggcataa cccaacggca tcacctgcgc cgtcacttcg ctaccgtcac tgctttgcca
    12061 caaaaactcg gttttatccg taccgtggcg ctcagaacat ccacgccaga acatggtgca
    12121 ggtaatgcca aatccattgt agatatgcgg cagttgcccg gacatgccaa aggaatcggg
    12181 taaataacct attttcatcg gctcgccaaa cgcgaggcag tcacgcattc cgtacatcag
    12241 attacggacg atggattccg cagaaacaat cgtggtatcg gtctgggtat accaggggcc
    12301 gataatcaac ttgccggctt ctaccagttt cttcacgcgg tctttgtttt ccgatttcac
    12361 cgcgaaataa tcttcgagga tcgccgtttg cccgtcgagt acgtaatatt tgtattcgtt
    12421 gtcctgttcc agtcggcaca ggatctcttc catattattg accagcagaa tgcgtgactc
    12481 ttcggtggtg aaataccact cccgatccca gtgcatatgc ggggtgatgt gaacgcgaga
    12541 taccgctttc atcgtcgttt cctttttgtt tatggcatta cgccatcagt cagatagttg
    12601 ccatgcttaa ccgcgtgacg ccgccagatc aggagaattg cagtcgagat tgcagccccc
    12661 accaatgccg caccaaacca gccaattgcc gccataacac cgcccgcgcc attatcatga
    12721 agtaaaaaga gcgagaaaat gccagcaccg ggtgtcgaaa ggccgatatt catcgcaccg
    12781 acaatagcgc ccgttaccat agagcccagc acaaacgaac caataacccg cagcggatct
    12841 tcaatcgcca tcgggatcgc cccttcggta atacctgcca gccctaacag ccaggtggat
    12901 ttcccggtct caatttcaaa ctctttaaac aggcgcggtg cgagcatcgt ggaagcggtt
    12961 acggtaaatg ccgaaaccat tttgacggag gcgaaaatgg catacgggcc gtaaacgccg
    13021 ttcgccattg cgcccaagca gaatgcataa gcggctttat tcactggccc tccaaggtca
    13081 aaggaacaca taaaaccgag aatggcaccc agcaacagcg cgttacttcc tgacagaccg
    13141 ttcagctagg cggtaagcga gttattgatc caggcgacag gttcccccac cacaaacagc
    13201 atcagactgc ccgctcccaa tgtaccgagc accgggtaga gataaaaagt caggaagcca
    13261 ttgaatttac tgctaagacg caagtgattt ttcatccagc gtatcaagta accggctatc
    13321 aatccgccaa cgaccgcgcc gagaaacccg gagccgatca tgttggcggc aagtccagcc
    13381 gcaaagcctg gcgctaacgc ccggtttatc tgccagcgaa taggcggtat aggctgccag
    13441 caccggtacc atcaaaattc cgagcagccc gccgcccagt ttgcggtaca tccacagcca
    13501 ggagttttct tcattaaaca gatcttgtag cccgaagatt tgcgacagta ataccgcgac
    13561 cgccagcacc gtcccccccg cgacaatcaa cggtacggca aaagagatcc cgctcaacag
    13621 tgcctgtttc agctccgttt tgacgctttt caccggttgc gtatcttgct gtacggtacg
    13681 cgtctcatcg ccacgctcga gggtaagcga ttgttggatc aacgcttctg catggcgaat
    13741 cggctcggca acagacacag aaagcgcagg aatgccgtta aaacgctcac tctccttgat
    13801 ggcgacttca gccgcaaaaa tacaggcagt tgcactattg agttgatccg ccgttaaacg
    13861 cccttcaata ccgttagcac cttgcttttc aacgtagaca ttcacgccga gtttgcgtcc
    13921 ggctttttcc agatactccg cagccatgta ggtgtgagca atacccgccg ggcaggccgt
    13981 tacgcaaacg atagttggcg cgttggaaaa agaggcagaa ggttgcgtgc ctcccttgtc
    14041 atccagcgcc gagagcaatt catcaggcgt cgtcgccgac tgtatacgcg cccgaatctc
    14101 atcatccgcc aggcgcgtgg tcagcgctgt cagcagttgc atgtgcgtag tacccgcttc
    14161 attgggggga atcgccagca acaccactaa atcaaccgct tccgggccat ccacgccttc
    14221 ccactgaagc ggctcgctga gtgtggcgac cgcaaacgcc gcttctttta ccgcagcagt
    14281 tttgccatgc ggcacagcca acccttcacc taaggcggtc gggccaaggc tttcacggcg
    14341 atacacttct ttcagaaatt gctcagtact ggaaattttc cccagagcag caagacgttg
    14401 agtcaacgcg tgaatggcct cttcacggct ggtaaagcgc gcattcagac acaacgcatc
    14461 gcggtgggtc agagtcgtca gattcatcat gatcctcact tgatcgtttt tatagtcgcg
    14521 ccagtgtgcc cgataaaaca acaccattgc gtgatcaatt tcacttgatt gaaattattt
    14581 acattgtatt tgtattattt acaggatgaa tttatcgggc ataatggcta aattcgtatt
    14641 aatgagatgc taaataatgg gacacaaacc cttataccga cagattgctg atcgcattcg
    14701 tgagcaaatt gcgcgtggcg agcttaaacc tggcgatgcg ttacctaccg aatcggcatt
    14761 acaaacagag tttggcgtca gccgggttac ggtgcgtcag gctttgcgcc agttagtcga
    14821 gcaacagatc ctcgaaagca ttcagggcag cgggacttac gtcaaagaag agcgggtcaa
    14881 ttacgatatt tttcagttaa ccagttttga tgaaaaactg tcggaccgtc acgtcgatac
    14941 acacagtgaa gttctgatat ttgaagtgat tccggctgac gattttcttc agcaacagct
    15001 acaaatcacc gtgcaggatc gtgtgtggca tgtgaagcgc gtgcgctatc gcaagcaaaa
    15061 gccaatggcg ctggaagaaa cctggatgcc gcttgcgttg ttcccggatc tcacctggca
    15121 ggtcatggaa aattcgaaat atcactttat tgaagaagtg aagaagatgg ttatcgatcg
    15181 tagcgaacag gaaatcattc ctctgatgcc aaccgaagag atgagccgtc tgcttaatat
    15241 cagccagaca aagccgattc ttgaaaaagt atcgcgtgga tatttagttg atggtcgggt
    15301 gtttgaatat agccgcaacg cttttaatac cgatgactat aaatttaccc tgatagcgca
    15361 gcgaaaatca tcgcgataag cacaaaaaag gccatcatac gatggccaac catgtcgaaa
    15421 ccgacagccc ttcgccattc ctgtagaggg aagcggcgag ggctatttct tattacagat
    15481 atttatttca gaacagtttt cagtgcttca ccgatatccg ccaggctgcg aacggttttc
    15541 acgcctgcgg cttccagagc agcgaatttc tcatccgcag tccctttccc accggcaatg
    15601 atcgcacccg cgtggcccat acgtttgcct tccggcgcag tcacaccagc gatgtaaccc
    15661 acaactggct tggtaacatg ctctttgatg tacgctgccg cttcttcttc agcgctaccg
    15721 ccgatctcac cgatcatcac gatcgcttcg gtctgcggat ctttttcgaa catttcgaga
    15781 atgtcgataa agttagagcc agggatcggg tcaccgccga taccgacaca ggtcgactga
    15841 ccgaaaccgt aatccgtggt ctgtttaacc gcttcatagg tcagtgtacc ggaacgggaa
    15901 acgataccca ctttacccgg tttgtgaatg tgacccggct ggataccgat tttgcattca
    15961 cccggggtga taacgcctgg gcagttcggg ccgatcatac gaacgcctgc ttcgtccagt
    16021 ttcactttca cggtcagcat atccagcgtc gggatgcctt cagtgatggt gataatcagt
    16081 ttgatgcctg catcgatagc ttccagaatg gagtctttgc agaacggtgc tggtacgtaa
    16141 ataacagacg cggaagcgcc agtagcagca acagcttcac gcacggtgtt gaacaccggc
    16201 aggccgaggt gggtagtgcc gcctttacct ggagttacgc caccaaccat tttagtgccg
    16261 tatgcaatgg cctgttctga gtggaaagtc ccctggctac cggtaaagcc ctggcagata
    16321 accttggtgt ttttatcaat taaaatggac attatttccc ctccactgcg gcaacaacct
    16381 gctgagctgc atccgtcaga ccttttgctg caataatatt caggccgctg tcagccagtt
    16441 tcttcgcgcc gagttcggcg ttgttacctt ccagacgtac cacgaccggt acgttaacac
    16501 ccacttccgc taccgcgccg atgataccgt cagcgatcag gtcgcaacga acgataccgc
    16561 cgaagatgtt aaccagaacg gctttcactt tgtcgtcaga gaggatgatt ttgaacgctt
    16621 cggttacacg ttctttggtt gcgccgccgc caacgtccag gaagttagcc ggttcgccgc
    16681 cgtgcagttt aactatgtcc atcgtaccca tcgccagacc tgcgccgtta accatacaac
    16741 cgatgttacc gtccagcgca acgtagttca gttcccactg tgcagcctgt gcttcacgcg
    16801 gatcttcctg cgactggtca cacatttcgc gcagatcagg ctggcggaac agggcgttgc
    16861 catcagcgcc cagtttgccg tcgaggcaaa tcagatcgcc ctgtttggtg atgaccagcg
    16921 ggttgatttc gatcagcgcc aggtcgcgct ccaggaaaat ggtcgccagg cccatgaaga
    16981 ttttggtgaa ctgctgaacc agtttacctt ccagacccag tttgaacgcc agctcgcgtc
    17041 cctgatacgg catcgggcca gtcagcggat caagcgcaac tttatggatc aggtgcggag
    17101 tttcttccgc cactttttcg atttccacgc cgccttcggt ggaggccata aagaccacgc
    17161 gacgggaact acggtctaca acggcaccga gatacagctc tttagcgata tcggtcgccg
    17221 cttcaaccag aatctggtta accggttggc cattggcatc tgtttgatac gttaccagac
    17281 gcttgcccag ccagttttct gcaaaagcac ggatgtcttc tttgctgttt acaactttca
    17341 caccgcccgc tttaccgcgg ccaccagcgt gaacctgaca tttcactacc cacggaccgg
    17401 caccgatttt tgaagcggct tcttctgctt cgcgcggagt agtacaggca taacccaccg
    17461 gtgctggtaa gccatagcgg gcaaacagtt gttttgcctg atattcatgt aagttcatgt
    17521 gttctgtcca tccttcagta atcgttatct tttaaaccgt agaccgacac cctatccacg
    17581 tcggtctaca gtgcaggtga agcttaaact actacacgtc cagcagcaga cgcgtcggat
    17641 cttccagcaa ctctttgatc gttaccagga agcccacgga ttcgcgacca tcgatcagac
    17701 ggtgatcgta ggacagcgcc aggtacatca tcggcaggat ctcaacctga ccattcaccg
    17761 ccatcggacg atctttgata gcgtgcatac ccagaattgc gctctgcggc gggttgatga
    17821 tcggcgtaga catcagggaa ccgaacacac caccgttggt gatggtgaag ttaccaccgg
    17881 tcagatcttc aaccgtcagc ttgccatcac ggcctttgac tgccagctct ttgattttct
    17941 tctcgatgtc tgccatgccg agggtatcga catcacgcag aaccggcgtc accaggccgc
    18001 gcggcgtaga aaccgccatg ctgacgtcga aatagttgtg gtaaaccacg tcatcgccgt
    18061 cgatagacgc gttcacttcc gggtaacgtt tcagggcttc aaccaccgct ttcacgtaga
    18121 aggacataaa gcccagacgg atgccgtggc gtttttcaaa cgcttcaccg tactgcttac
    18181 gcagatccat aatcggcttc atgttgactt cgttgaacgt ggtcagcatg gcggtggagt
    18241 ttttcgcttc cagcagacgc tctgccacac gcttacgcag gcgagtcatc gggacacgtt
    18301 tttcactacg tgcagccaga gccggttgcg ccgccggagc agccgctgcc ggagcagact
    18361 ctttcgccgg ggctttcgcc agatgttttt ccacatcttc acgagtcaga cgaccaccca
    18421 caccggtgcc tttaatggcg ctggcgtcga gattgtgttc agccagcagg cgacggatcg
    18481 ccgggcttaa cgcatcgttg ttttgctctt ccagagacgc ctgctggcgt tgcgccggag
    18541 tggacgcttt ctcttcagat ttggcgctgg tttctttacc ggcgctgttg ccttcacgca
    18601 ggcgaccaag gatctgacga gacgttaccg ttgtaccttc atcttccaga accgcatcca
    18661 gaatgccgtc tgctgatgcc ggtacttcca gtaccacttt gtcagtttcg atttctacca
    18721 gcacttcatc acgtacgact gcgtcgccgg gttttttatg ccaggttgcg acggtggcat
    18781 cggctacgga ttcaggcagg tcagggacca gaatatctac gctactcatt gtgtatcctt
    18841 tatttattcg acgttcagcg cgtcattaac cagatcttgt tgctgtttct ggtgaacgga
    18901 catataccct accgccggag aggcggaggc cgggcggcct gcataacgca gagaagcccc
    18961 aaacggaatc acttcacgga aatgatgctg gctgcagtac catgcgccct ggttgagcgg
    19021 ctcttcctgg caccagacaa aatccttgac gtgagcaaac tgctgcaaca cttcctgcat
    19081 cgctttatgc gggaacgggt agagttgctc gatacgcaca atggcgacat cgtgttgatt
    19141 gttcttacga cgctgttcca gcaggtcgta ataaacctta ccagaacaca tcactacgcg
    19201 cttcacgccc ttcggatcaa gctcgtcgat ttcaccgatg gctggcagga aggtgccgtt
    19261 cgccagttct tcgaggcagg aaaccgccag cggatgacgc agcagggatt tcggcgacat
    19321 cacgaccagc ggacgacgca tcccgcgcag cgcctgacga cgcagcatgt ggtaaacctg
    19381 tgccggggta gacggtacgc aaacctgcat gttttgctca gcacaaagtt gcagataacg
    19441 ttccagacgc gcggaggagt gctccggccc ctgcccttcg taaccgtgcg gcagcaacat
    19501 caccagacca cacatccggc cccatttctg ctcgccagag gagatgaact ggtcgataac
    19561 cacctgcgca ccgttggcga agtcaccgaa ctgcgcttcc cagatggtca gggtgcgtgg
    19621 ttctgcggtg gcataaccat attcaaacgc cagcacggct tcttctgaca gtacggagtc
    19681 ccagacacgg aacgcgccct gaccgttatg gatgtgttgc agcggcgtgt aagtggaacc
    19741 gttagactgg ttgtggatca ccgcgtggcg gtggaagaag gtaccgcgac cggagtcttc
    19801 acccgacagg cgaaccggaa tgccttcatc aaccagcgtg gcgtaagcga ggttttccgc
    19861 accgccccag tcgaacagtt tctcaccggc tgccatcgcc tggcgatcgc cataaatctt
    19921 ggcaacgcga gactgcattt caactgcttc tggtaccgta ctgatacgtt tagccagttc
    19981 ctgcaggcgc ttcatctcaa ctttgttcgg gtactcttcg tcccattcgt ggttgaggta
    20041 cggcgaccag gtgaaagagt gcatgttcat cggacgccac tctgccacta cgcaatcgcc
    20101 agcatccagc gcatcgcggt acaggttaac catctcggtg gcatcttcca gcgtcaccac
    20161 tttttcctgc tccagcttgt cagcgtagat tttgcgcggt gtcggatgtt ttttgatttt
    20221 ctgatacatc agtggctggg ttgcgctcgg ctcgtcggct tcgttgtggc cgtgacggcg
    20281 gtagcatacc aggtcgatga agacatcacg tttaaaggtg ttacggaaat cgagcgccag
    20341 acgggtcaca aaggcaacgg cttccggatc atccgcgtta acgtggaaaa tcggtgcctg
    20401 aaccatctta ccgatatcag tacagtacgg tgtagaacgg gcatccagcg ggttagaggt
    20461 ggtgaaacca acctggttgt tgataacgat acgtaccgta ccgccaactt cataaccacg
    20521 cgctttcgac atgttcagag tttcctgaac cacgccctgc ccggtcactg cggcgtcacc
    20581 gtggatggtg attggcagca ctttgttgct gctcggctca tcaagtctgt ccagacgagc
    20641 acgaacagag ccgataacga ccgggcttac aatctcaagg tgagacgggt taaacgccag
    20701 cgccaggtgc accaggccgc catcggtctg gaagtcagac gagaagccca tgtggtattt
    20761 cacgtcgcct gtgccgaggt gttctttatg tttaccggcg aactcgtcga acaagtcttg
    20821 cggtttttta cccagcacgt tcaccagcac gttcagacga ccacggtgcg ccatcccgag
    20881 aaccacttcg cgggtgccgc tgttgccagc gtggcggatc atctctttaa gcatcgggat
    20941 taacgcgtca ccgccttcca gcgagaagcg ttttgcgcca gggaattttg cgccgaggta
    21001 acgctcaagg ccttcagcgg cggtcagttc gcttaagaag cgttttttct cttcgctatt
    21061 gaaagtcgcg cgaccagact cgatacgctg ttggatccag cgtttttctt cggtgctggt
    21121 gatgtgcata tactcggcac caatcgggcc gcagtaggtt tgcttgaggg cggaaataag
    21181 ctcgccgagt ttcatggttt ctttgccgct ggcaaatgaa ccgacgttga aggtttcctg
    21241 gaagtctgct tcggtcagat cgtggaaaga cggatccaga tcggccactt tatcttgctg
    21301 ccacagtccc agcggatcta gattcgcatg ctggtgacca cggaagcggt atgcgttaat
    21361 gagctgcagg actttaacct gcttcacatt ggtatcaggg tcggagatcg ttgaagagta
    21421 acgtgaagcg tctttcgcca ggcggcggaa atattcacgc gtttgagagt ggaattgatc
    21481 cggtttgact cccgtaccag gtaactgctg gaacgtcgaa cgccagttag cgtcaaccga
    21541 gtcaggatcg gttaagaagt cttcatagag ctgttctatc cagctctggt ttgcgccaga
    21601 gaggtaagaa gagtccaacc aggctttcaa agcgctgttc tgcatcgtga tcccttaagc
    21661 atctttttta tgcttacttc gccgtggata ctaccacgca cagtgatgtg cgtgccgggg
    21721 ttcacttgcg agctcttgtc tacgtagtgg ctcgcgaagg aacctttaaa aactgtctta
    21781 gtgtcagggg cagtttttaa agatttcctg catttctttg ttacgcctga tgcgctgcgc
    21841 ttatcaggcc tgcgggtaat gacttgtagg ccggataagg cgttcacgcc gcatccggca
    21901 ctggttgcct gatgcaacgc ttgcgcgtct tatcaggcct acagtttacg cattacgttg
    21961 caacaacatc gacttgatat ggccgatggc gcgcgtcggg ttcagcccct tcggacatac
    22021 actgacgcag ttcatgatgc tgtgacagcg gaatacgctg aatgcatcgc tcaaaccgtc
    22081 gaggcggctg tcagtctcgg tatcacggct atcgatcagg aaacgatatg ccgctaacaa
    22141 gcctgccggg ccgataaact tatcgggatt ccaccagaaa gacggacaag aggttgaaca
    22201 acatgcgcag agaatacatt catacagccc gtcgagtttt tcgcgctgct ctggcatctg
    22261 taaatgctcg cgagctggcg gattttgtcc attattcaac aggtaaggct taattttctc
    22321 atattgcgca tagaattgtc ccatgtctac caccaaatcg cggatcaccg gtaaacctgg
    22381 cagcgggcga atcacaatct tcttgcccgg ctggttgagt gccgaaatcg gggtaataca
    22441 ggccagacca ttcttgccgt tcatgttcag accgtcggaa ccgcacacac cttcacggca
    22501 ggagcggcgg aacgacaggc tgggatcttt ctctttcagc tggataagcg catccagcag
    22561 catcatgtca cgaccttctt ccgcttccag ggtgtaatcc tgcatacgcg gagcatcatc
    22621 aacatccggg ttatagcgat aaattgaaaa ctcgagtctc attttcctgt ctccgcatta
    22681 gtaagtacga atcttcggcg ggaatgccgg gcgcagtttc ggttccatgt tgacgcttcg
    22741 gcgcgtcatg gattccgact caggcagata cagggagtgg cacagccagt tttcatcatc
    22801 acgatccggg aagtcgaagc ggctatgtgc gccacggctt tcggtacgga agttggcaga
    22861 aacagccgtt gcatacgccg tttccatcag gttatccagt tccaggcact caacgcgctg
    22921 ggtgttgaac tcgctggaag tgtcatccag acgggcattt ttcagacgct cgcggatcac
    22981 tttcaactgc tcaagccctt tcgccatcgc atcaccttca cggaagaccg agaagttatg
    23041 ctgcatacat tcttgcagcg ctttacggat cgccaccgga tcttcaccgt tacgattatt
    23101 gttccagcgg ttcaggcgat ccagagacgc ttcaacatca gactcgctgg catcgcgcag
    23161 tgcgccctgc tcggcgatag actcttgcag atgcagacct gccgcgcgac caaagaccac
    23221 caggtccagc agcgagttgc cgcccagacg gttagcgccg tgtaccgata cacaagcgat
    23281 ttcaccaacg gcaaacagtc ccggaacaac cacatcttcg cctttctcat tcacagtcag
    23341 cgcctgaccg gtaactttgg tcggaatacc gcccatcatg tagtgacagg ttgggataac
    23401 cggaatcggc tctttcaccg gatcgacgtg agcgaaggta cgggaaagct ccaggatacc
    23461 cggcagacgg gattcgagaa cttctttacc caggtgatcg agtttcagtt tcgcgtgtgg
    23521 cccccacgga ccatcacagc cgcgaccttc acggatttca atcatgatgg aacgcgccac
    23581 cacatcacgg cccgccaggt ctttggcgtt cggcgcataa cgctccataa agcgttcgcc
    23641 atgtttgttc agcagataac cgccttcacc acggcaacct tcggtgacca gtacgcccgc
    23701 accggcaatg ccggtcgggt ggaattgcca catttccata tcctgcaccg gtacgcctgc
    23761 gcggatagcc atgccgacac cgtcgccagt gttaatgtgg gcgttagtgg tggactgata
    23821 aatacgccct gccccgccag tcgccagcac ggtagcgcgg gctttgaaat aaaccacttc
    23881 accggtttcg atgcacagtg cggtacaacc caccaccgcg ccatcctggt ttttcaccag
    23941 atccagcgca taccactcgg agaaaatggt ggtgtggttt ttcaggttct gctgataaag
    24001 cgtgtgcaac agtgcgtgac cggtacggtc agcagccgcc gcagtgcgtg ccgcctgctc
    24061 gccgccgaag tttttcgact gaccgccaaa cggacgttga tagatacgac catcatcgag
    24121 acgcgagaac ggcaggccca tgtgttcgag ttccagaatc gcttccggcc cggttttaca
    24181 catatattca atcgcgtcct ggtcaccgat atagtccgac ccttttacgg tgtcgtacat
    24241 atgccattcc cagttatctt catgggtatt acccagcgca acggtaatgc cgccttgcgc
    24301 agaaacggta tgggaacggg tcgggaagac tttagagagc agcgcacagg tctggccgct
    24361 ctgggaaatt tgcagcgccg cgcgcatacc tgcgccaccg gcaccaatca caactgcatc
    24421 aaattctctg actggcaatt tcatcacaca ccccacacca caacgaatcc ataaatcacg
    24481 taaaccacca gtgcaacgac aatcaccagt tgcagcatca ggcgcaaggc cagcggttta
    24541 acgtagtcgg tcaacacctg ccacatgccg atccaggcat gaatcaagat agaaaacagc
    24601 gccagcaggg tgaacacttt ggtgaacgca gaggcgaaga aaccgatcca gacttcatat
    24661 gtcagttcgc cactggtggc gaaaaaaccg accatataaa tgatgtagag cgtcaggacg
    24721 atagcggtag cgcgaacgag gatgaaatca tgtacgccat tgcgtcctaa tgcggaggcg
    24781 ttgcttacca tacgaggact cctgcgagaa gtgaaagcac gacagtaata acaaaggaga
    24841 ttttggcgga gcgtttaccc gcttcgaatg tttcttccag atagccaaaa tccatcatca
    24901 tgtggcgaat acctacgacg acgtgatacg ccagagcggt aaggatgccc cacatgataa
    24961 atttgacgaa gaagctgccc ataatcgcgg aagcttgctc gaaaccttca ggggaagaga
    25021 ggctggtacc cagaagccac agcaggatgc ccactgcaac aaaggtgatc acaccggaaa
    25081 cgcgatggag aatggacgct atcgccgtga tggggaaccg gatggtctgt aggtccagat
    25141 taacaggtct ttgttttttc acatttctta tcatgaataa cgcccacatg ctgttcttat
    25201 tattccctgg ggactacggg cacagaggtt aactttctgt tacctggaga cgtcgggatt
    25261 tccttcctcc ggtctgcttg cgggtcagac agcgtccttt ctataactgc gcgtcatgca
    25321 aaacactgct tccagatgcg aaaacgacac gttacaacgc tgggtggctc gggattgcag
    25381 ggtgttccgg agacctggcg gcagtatagg ctgttcacaa aatcattaca attaacctac
    25441 atatagtttg tcgggtttta tcctgaacag tgatccaggt cacgataaca acatttattt
    25501 aatttttaat catctaattt gacaatcatt caacaaaatt gttacaaaca ttaccaggaa
    25561 aagcatataa tgcgtaaaag ttatgaagtc ggtatttcac ctaagattaa cttatgtaac
    25621 agtgtggaag tattgaccaa ttcattcggg acagttatta gtggtagaca agtttaataa
    25681 ttcggattgc taagtacttg attcgccatt tattcgtcat caatggattc tttacctgca
    25741 agcgcccaga gctctgtacc caggttttcc cctctttcac agagcggcga gccaaataac
    25801 aaacgggtaa agccaggttg atgtgcgaag gcaaatttaa gttccggcag tcttacgcaa
    25861 taaggcgcta aggagacctt aaatggctga tacaaaagca aaactcaccc tcaacgggga
    25921 cacagctgtt gaactggatg tgctgaaagg cacgctgggt caagatgtta ttgatatccg
    25981 tactctcggt tcaaaaggtg tgttcacctt tgacccaggc ttcacttcaa ccgcatcctg
    26041 cgaatctaaa attactttta ttgatggtga tgaaggtatt ttgctgcacc gcggtttccc
    26101 gatcgatcag ctggcgaccg attctaacta cctggaagtt tgttacatcc tgctgaatgg
    26161 tgaaaaaccg actcaggaac agtatgacga atttaaaact acggtgaccc gtcataccat
    26221 gatccacgag cagattaccc gtctgttcca cgctttccgt cgcgactcac atccaatggc
    26281 agtcatgtgt ggtattaccg gcgcgctggc ggcgttctat cacgactcgc tggatgttaa
    26341 caatcctcgt catcgtgaaa ttgccgcgtt ccgcctgctg tcgaaaatgc cgaccatggc
    26401 cgcgatgtgt tacaagtatt ccattggtca gccatttgtt tatccgcgca acgatctctc
    26461 ctatgccggt aacttcctga atatgatgtt ctccacgccg tgcgaaccgt atgaagttaa
    26521 tccgattctg gaacgtgcta tggaccgtat tctgatcctg cacgctgacc atgaacagaa
    26581 cgcctctacc tccaccgtgc gtaccgctgg ctcttcgggt gcgaacccgt ttgcctgtat
    26641 cgcagcaggt attgcttcac tgtggggacc tgcgcacggt ggtgctaacg aagcggcgct
    26701 gaaaatgctg gaagaaatta gctccgttaa acacattccg gaatttgttc gtcgtgcgaa
    26761 agataaaaat gattctttcc gcctgatggg cttcggtcac cgcgtgtaca aaaattacga
    26821 cccgcgcgcc accgtaatgc gtgaaacctg ccatgaagtt ctgaaagagc tgggcaccaa
    26881 agatgacctg ctggaagtgg ctatggagct ggaaaacatc gcgctgaacg acccgtactt
    26941 tatcgagaag aaactgtacc cgaacgtcga tttctactct ggtatcatcc tgaaagcgat
    27001 gggtattccg tcttccatgt tcaccgtcat tttcgcaatg gcacgtaccg ttggctggat
    27061 cgcccactgg agcgaaatgc acagtgacgg tatgaagatt gcccgtccgc gtcagctgta
    27121 tacaggatat gaaaaacgcg actttaaaag cgatatcaag cgttaatggt tgattgctaa
    27181 gttgtaaata ttttaacccg ccgttcaaat ggcgggttaa tttttatatg cctaaacaca
    27241 aaaaattgta aaaataaaat ccactatcgg acattcatat attctaaaaa aaatatatcg
    27301 gtataaatta taaacaaatc gatgattttt aatcatctat cttcctctca gttacatgaa
    27361 taaaccacca aatatagcgc acccctataa tttccaatac tttattcatc aaacatttaa
    27421 ttcataccat caattaatta cttttacatt taataaaatt aataatattt ttaagaaaaa
    27481 ctaaaaacat tcgacaatca aataagatat tcttgcaccg atatactttt ttcttttctg
    27541 ttaaaggact cagttatgtt taaaggacaa aaaacattgg ccgcactggc cgtatctctg
    27601 ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca ctttaaaggt
    27661 gaagttattg aagcaccgtg tgaaatacat caggatgata ttgataaaga ggttgaactc
    27721 ggtcaggtga ccaccagcca cattaatcag tcacatcaca gcgatgccgt tgctgtcgac
    27781 ctgcgcttag tcaactgtga tctggaaaac tccagcaacg gttccggtgg caagatttct
    27841 aaagttgcag tcacctttga tagctcagcg aaaaccaccg gcgcagatcc gattctcaac
    27901 aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa agatcaaagc
    27961 aatatcgttt taggcaccgc tactccagat atcgacctgg ctccgacctc cagcgaacaa
    28021 acgctgaatt tctttgcctg gatggaacaa attgatcagg ctacacctgt aacgccaggc
    28081 gcagttacgg cgaatgcaac ctacgtgctg gattataaat aagtattact ttggcggcgc
    28141 tatcgccgcc tttcataaaa tatttatcag gtatggacac cgtgaatatt tatcgactct
    28201 cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa ttcaatctga
    28261 atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag gaaaaaggag
    28321 tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa atcagtaatg
    28381 ggcaaaagat taactggcac aaaaatgacg ataaaaccat tccgtgcatc aatgatttac
    28441 tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg ataaatcagt
    28501 gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc aatcagcaac
    28561 tgaatattag tattccgcaa gcctggctgg cgtggcactc agaaaactgg accccaccct
    28621 ccacatggaa agaaggtgtc gccggtgtcc tgatggatta caacttgttt gccagcagct
    28681 accgcccaca ggatggcagc agcagcacta atcttaacgc ctatggaaca gccggaatca
    28741 acacgggggc atggcgctta cgcagtgatt atcagttgaa tcatactgat agcgatgata
    28801 accatgaaca gtcaggcgaa atatcacgta cctatctttt tcgcccgtta ccgcaattag
    28861 gttcaaaatt aaccctcggc gaaacggatt ttagttccaa tattttcgac ggtttttctt
    28921 ataccggcgc ggcactggca agtgacgatc gaatgttgcc atgggaacta cgcggctacg
    28981 ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt caatcaggcc
    29041 gcgtcattta ccagaaaaaa gtcccaccag gcccatttat cattgacgac cttaatcagt
    29101 ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg aacaatttcc
    29161 aggtttcagc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc tataaactgg
    29221 ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc ttttttagca
    29281 atgaagtttc ctgggggatg ctgtcaaaca cctcgctgta cagcggcctg ctgctttctg
    29341 gtgatgacta ccattctgcc gcaatgggta ttgggcaaaa tatgctgtgg cttggtgcgc
    29401 tgtcgtttga tgtcacgtgg gccagtagcc attttgatac tcagcaggac gagcggggct
    29461 taagctaccg ttttaattac agcaaacaag tggatgccac taacagcacg atttccctcg
    29521 ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctg gatcacaaat
    29581 acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg ggccaaccga
    29641 ttaccccact aaacttcaat ctttacgcca acctgctaca tcaaacctgg tggaatgcag
    29701 acgcctccac gaccgccaac atcacggcag gttttaatgt tgatattggt gactggagag
    29761 atatctctat ttcgacgtca ttcaatacaa cccattacga agataaagat cgcgacaacc
    29821 agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc tatgacatgc
    29881 aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg gatgaacgta
    29941 atagctgggg catgtctgcc ggactgcaat ccgaccgtcc tgacaatgga gcccaggtga
    30001 gcggtaacta tcagcacctg agttcagcgg gtgagtggga tatttctggt acctatgccg
    30061 ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca acccaatatg
    30121 gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc agcaccgatg
    30181 gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt ggcattgcgg
    30241 tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggaagacgtt aacgtcatca
    30301 ttcgcaacgc cagcggtcag tttcctcccc tcggagcgga tatccgccag gatgacagcg
    30361 gcattagcgt ggggatggtt ggcgaggaag gacatgcctg gttaagcgga gtcgctgaaa
    30421 atcaaaaggt taccgtggtc tggggtgata gccagcattg ctcgctccat cttcctgaac
    30481 atatggaaga caccgcaaat cgcctgattt taccttgtca ttaattaagg aaatgaaaat
    30541 gacatttatg aaaggactac cccttttgtt gttggttgcc agtctgtgca gccacgctgc
    30601 actacaaccc gatcgcactc gtattgtgtt taacgctaat gataaagcta ccagtctgcg
    30661 ggtggataat cgtagcgata aactccccta cctcgcttat tcctggctcg aaaatgaaaa
    30721 aggggagaaa agcgatgatc ttctggtcgc cctgccgccg atacaacgtc tggaaccgaa
    30781 agcaacaacg caggtacgga tagtaaagca agcctcgacc acaaaattac cgggcgatcg
    30841 cgaaacgcta tttttctaca atatgcgtga aattcctccc gccccggaaa aaaacagcga
    30901 ccatgccgtt ctgcaagtcg ccattcagag tcgtatcaag gtgttctggc gaccggctgc
    30961 attacgcaag aaagcaggag aaaaggtcga actgcagtta caggtcagcc agcagggcaa
    31021 ccaactgaca ctgaaaaatc ctaccgcgta ttatctgacc attgcctacc tgggacgaaa
    31081 tgaaaaaggc gttctccccg gcttcaaaac cgtgatggtt gcgcctttta gtacggtcaa
    31141 cacaaacacc ggaaattata gcggcagcca gttttatctc ggttatatgg atgattacgg
    31201 cgcgttacgc atgacgacgc tcaactgcag cggacaatgc catttacaag ccgtggagac
    31261 gaagaaatga gtgctggcaa gggagtgttg ctcgtcatct gtctgttatt tctgccgttg
    31321 aagtctgcgc ttgccctgaa ctgctacttt ggttcatccg gtggctcggt agaaaaatca
    31381 gaagcgattc aaccttttgc tgtaccaggc aatgccaaac tcggcgataa gatctgggaa
    31441 tctgacgata ttaaaattcc cgtctattgt gacaacaata ccaacggcaa ctttgaaagc
    31501 gaacatgtct acgcatgggt aaacccctac ccgggggtgc aggatcgtta ttatcaactc
    31561 ggagtaactt acaatggcgt tgattatgac gccaaccagg gaaaaagccg catcgacact
    31621 aaccagtgta tcgacagtaa gaacattgac atttataccc ctgagcagat catcgcgatg
    31681 ggatggcaga acaaaatttg ctcgggtgat cccgctaata ttcacatgtc gcgtaccttt
    31741 ctcgctcgca tgcggttata cgtcaaaata cgagaaatgc cgccgcatga ttatcaaagt
    31801 acgcttagcg actatatcgt cgtgcaattt gacggtgccg gtagcgttaa cgaagaccct
    31861 actgcccaaa acctgaaata tcatattact ggtctggaaa acattcgcgt gctggattgc
    31921 agcgtcaatt tttccatttc accggaaaca caggtgattg attttggtaa atttaatttg
    31981 ctggatattc gtcgccacac aatgtcgaaa acattcagta ttaagacgac aaaatcacaa
    32041 aatgaccaat gcaccgacgg atttaaagtc agttcctcct tttataccga agaaacgttg
    32101 gttgaagaag ataaagcatt gttgattggt aatggtctga agctacgttt gctggatgaa
    32161 aacgcctcgc cttatacctt caataaatat gctgaatacg ccgatttcac cagcgacatg
    32221 ttggtctacg aaaaaaccta tacagctgaa ctttcgtcca ttgcaggtac ccccatcgag
    32281 gctgggccct tcgatacagt ggtgcttttt aaaattaact ataactgatt tgccatgatg
    32341 ccctttgcag ccggaaaggg catctctttc agaaaattgc tgattttccc cttttccttg
    32401 attgctgtta ttttttccta gcccggcttt ctttatgctg tatcgataca ccaaagagaa
    32461 aataatgagg gagcgaagga tgccagtttt gcagtgggga atgttatgtg tgctatcact
    32521 tctcctttct attggtttcc tcgcggtaca tctcccggcg gcgctattac ttgggccgat
    32581 gatcgccggg atcatcttca gtatgcgcgg aattactctg caacttcccc gctccgcttt
    32641 tctcgccgcg caggccattc ttggctgcat gattgcgcaa aacctcactg gttcgattct
    32701 caccacgctg gccgccaact ggccgatcgt gcttgctatt ttactggtga cgctgctttc
    32761 cagcgccatc gtgggttggt tattggtgcg ctatagctca ctgcctggaa ataccggtgc
    32821 ctggggttct tctccaggcg gcgcagcggc aatggtcgct atggcgcaag attacggcgc
    32881 agatattcgc ctggtggcgt ttatgcaata tctgcgagtg ctgttcgtcg cgggggccgc
    32941 tgttctggtt acccgcatga tgctgggtga taacgctgaa gcggttaacc agcaaatcgt
    33001 ctggttcccg cctgtgagca ttaatctcct gcttacgatt ttgctcgcag tcgttgccgg
    33061 tacggcggga tgcatgctgc gtcttccttc cggcacgatg ctcatcccga tgctggcggg
    33121 cgcagtgctc cagtctggtc agctcatcac catcgaacta ccggaatggc tgctggcgat
    33181 ggcgtatatg gcaattggct ggcggattgg tcttggtttc gataaacaaa tcttactgcg
    33241 ggcactacgc ccgctaccgc aaatcctgct gtcgattttt gctctgctgg ctatttgtgc
    33301 aggtatggcg tggggactga cccggtttat gcatattgat tttatgactg cctacctcgc
    33361 caccagtccc ggcgggcttg ataccgtggc ggtcatcgcc gcagggagca atgccgatat
    33421 ggcgcttatt atggcgatgc aaaccctgcg cctgttcagt attttgctga cggggccagc
    33481 cattgcgcgg tttatttcaa cctatgcgcc gaagcggtcg gcctagtgct ggcagcctgg
    33541 acaccagtaa aacgggcgcg aggacaacgt ggttttctca atgatgccgc cacagcgttc
    33601 gcacagttcg ccatcacgat gaaaaacctt aaagcgaaac agcgccccat gatgcttatt
    33661 ctcatccacc tgcccccgcg tagcgtaaga aaaccgagga atctccagta acgcgtgtgc
    33721 gagtgcatcc agttgcgccg cattgagatc tttcgcttta tgatttccag tcaacccaac
    33781 ctgccagagg atctccaccc gcaaataatt gccaagccca gccagaaacg cctgatcgag
    33841 cagtaatcct gcaaactgac ggttacgaaa gcgcggcgac aataaccgtt cctttaccac
    33901 ctccggcgtc agattcggat ccagcacatc gggaccaacg cgttgtaaaa acggatgcgt
    33961 ggtcagttgt tccggcctca acatctcaat atccgaggcg ctataaagca gaatggtttt
    34021 gtcagccgtt tgcagtttta cccgcaatac tcgcgtggtc tgcggctctt cgccggtatc
    34081 aaccacgcgc cagacgccgt aaagctgatt atggctgtag agcgttaaac cgttgggaaa
    34141 atgagttaac aacgccttac cacgcgtttc cacatgggta acgtgttgac cgataagttg
    34201 tgattgataa gtttttaact gcgggaaggc aaaccagaca tcagttagtg gtttgccttt
    34261 gatcgccgcc tccaggttat ccgctgcacg gcggatctcc gggccttcag gcatggtgat
    34321 atcctttttt cattctttat taagcggggt tatgctgcga caacaatccc ctttttggca
    34381 aatgtagctc gtaatcggcg ggcgaacgcc agagcgtgct cgccgtcgcc atgcaggcag
    34441 accgtttgcg ccgcgaccgt tgcccattcg ccggtgatac ttttgactct gccgtgttgc
    34501 accatctcca gcgtttgcgc caacgcctgt tcttcgtttt caatcaacgc gcctggctga
    34561 cttcgcggca ccagcgagcc gtcagcctga taaccgcgat cggcaaacac ttcctcgcgc
    34621 gttgtcaggc catattgctt gcctgcacga atcagctcgc ttcctgccag cccaacgaga
    34681 accaatgccg gatcgcaagc gtataccgct ctggcgatgg cgtctgccag ttgtgcttct
    34741 ttcgccgcct ggttgtacaa catgccgtgc ggtttgacat gacgcattac gccgccttgc
    34801 gcacgggcaa tcgttgccag cgcgccaatt tgatacagcg tctgggcgta aacggtttcc
    34861 ggcggcagct gcatggcgct gcgaccaaaa ttttccctgt cgggaaaact cgggtgagcg
    34921 ccaatcgcga caccattttt tattgcttca cgcacgcaag cctgcatggt ttgcgcatcg
    34981 cctgcatgaa atccacaagc aatattggca gaggaaacca gcgttaatag ctctgcgtcg
    35041 ctggcgcagc cttcgcccag atcggcgttc aggtcaattt tcattgtgca gccgccacgc
    35101 taattgttca aaataacgtt gctgatcttg ccgcgctttc agtgcctctt ccagtgaaca
    35161 ctggacaaaa tgaatcggct gaccgagcgg aatttgcgcc agatggtaca tatcagcctc
    35221 aatgatacag gcaatacgtg gataaccacc ggtggtctgt gcgtcgttca tcaacacaat
    35281 cggctgcccg ttatgcggca cctgcaccac gcccggtaac aaaccgtgaa ataacagttc
    35341 gcgatcggtg atgcgtttta aaatttgccc ctgtaagcga tagcccattc ggttactttg
    35401 cgagctaagc tgccagggcg aacgccagaa tgcatcctgc gaggcgcgat cgaactcatg
    35461 atattccggc cccggcaagg cgcgaatgcg gttgccccac agcagctgtt taacgccctg
    35521 cgcttccata aaatcacgct tcgatttgcc aatcgggagt cggtcaccat ccttcagtaa
    35581 acggccttcc agcccgccaa tccccacttt gagatcggtg ctgcatgacc ccattaccgg
    35641 cggaacatca ataccacccg caaccgccag ataactgcgc atcccgtgct gcgggcgttt
    35701 aagcgttaaa cgctggcctg ctttcatcgg caatcgccag ccggtccaga cggcgttatc
    35761 atccaaccgc gcttcgcaac ctgcacccgt cagggcaaac cacccatcag tttcgaactc
    35821 aactgttaac tgaccgagcg tgatctccag cgcgggggca ttggcgtcat tacccaccag
    35881 caggttagca atgcgcagcg caggcatatc cagtgcgcca cagtggctga tacccgactg
    35941 gcgaaaaccg tgacgaccgc catcctgcac agtggtgtac atgcccgcac gaataatctt
    36001 cagcatattc cctccttctg cggtacaaat cgcacgctgt ctcccggacg taataagatg
    36061 ggttcgtcac gcgccgaatc aaacaggctg agtgaggtat gaccaatcaa ctgccagcca
    36121 cccggcgttg ccagcggata aacaccagtc tgcggcccgc cgatcccgac agaacctgcc
    36181 ggaacgagca agcgcggttc agcgcgccgt ggcgtgtgta gttgttccgg caaactcccg
    36241 agatacggga agcccggttg aaaacctaaa aaccagacca cgtattccac ggaggagtgc
    36301 aattcaacaa cctgtttttc gctcaacccg caatacgccg cgaccaccgc caaatccggt
    36361 ccgcctgcac caccgtaaac caccggaatt tcaataaagc gagactcagg ttccagggcc
    36421 tcgctctcct cccaccagcg ttgcaaacgc tctatggcat ccagcgccag cgactcagga
    36481 ttacgcaaaa tcaccgtgat attgttcatg ccgggaatgg cttcaaccac attcggcata
    36541 tccaccagac gctgcgccag tcgccagatc cgtttctggc tagccagcgt caccggcggt
    36601 tccagttcca gcactaccgc cgtttcacct atcagataac aacgcgctcg ttgcactttc
    36661 gatccctctg attattcgtt atgcaggatt aggaatatca ataaaggtca catcaagatc
    36721 ggtattttca ttcagccact cgctcaatgc gcgaatacca ccgcgttcag tggcatggtg
    36781 acctgcagca taaaaatgca atccctgctc gcgggctgaa tgaatggtct gttcagaaac
    36841 ttcgccagta ataaaagcat ccacgccaaa acgcgcggcg ctatcgataa aactttgccc
    36901 gccgcccgtg caccaggcga cgcgctggac cacctcaggt ccggtatcgc cacaccataa
    36961 cggcttacgt cccagacgcg cttcaatcca ggaggctagt tccattcccg gcacaggcat
    37021 ggtcagctct ccccacggca ccaacggctc aatttcgccc atgaccgtga tccccagtaa
    37081 cgccgccagc tgtgcgttat tgcccagctc aggatgtgcg tcaagcggca aatgccagcc
    37141 atacaggttg atatcattcg ccagcaacgt ttttaaacgg ttacgcttca ttccgcgaat
    37201 gaccggagac tctcctttcc agaagtagcc atgatgcaca atgaccgcat ccgcgcccag
    37261 acgcactgcc tcatcgagca aagcctggct ggcggtgaca ccggtaacaa ttttttgcac
    37321 cgtctctttg ccttccacct gcaaaccatt cggcgcgtaa tcactaatcg ccgcgctgtt
    37381 caatttttcg ttaatcagtt gttccagttc ggtgtttttc atcttcgcgc cttccgtatt
    37441 ctgatagcta caccgttaac atagcgttac ctcgcctgtt cgtcgatagc caatcttttg
    37501 acatattaag aaacaaaccc gacgccaaaa gacgcaaatt gtttatatca tcacgcttca
    37561 gctaatgtat atttatgcaa tcagacagca tattgcataa ataatcagat ctgtatcaaa
    37621 gttttaactt taaggaaaca caagcacgcc gcgcaactat tacgtgacgt tgccgtcaat
    37681 tgtggaatta ttatgaataa acacgcatca caaccgcgcg ctatttacta tgtcgttgcg
    37741 ctgcaaatct gggaatattt tagcttttac ggcatgcgtg ccctgctgat tctctatctc
    37801 accaatcaac taaaatacaa cgatactcac gcctacgagt tatttagcgc ctactgttcg
    37861 ctggtgtatg tcacgccaat cctcggtggc tttttggcgg ataaagttct cggcaatcgc
    37921 atggcggtga tgctgggggc gttgttgatg gcgatcggtc atgtggtgct gggtgccagt
    37981 gagatccatc cgtcattcct ctatctgtcc ctggcgatta tcgtctgcgg ctatggcctg
    38041 tttaaatcta acgtaagctg tctgctcggc gagctgtatg agccaaccga tccgcgtcgt
    38101 gatggcggtt tctcgctgat gtatgcggcg ggtaacgtgg ggtctattat cgcacctatc
    38161 gcctgtggtt acgcccagga agagtacagc tgggcgatgg gctttggcct ggcggcggtt
    38221 ggcatgatcg cgggtctggt cattttctta tgtggcaatc gtcatttcac tcatacccgc
    38281 ggcgttaaca aaaaagtact gcgtgcgaca aactttctcc tgccgaactg gggatggctg
    38341 ctggttctgc tggtggcaac gcctgcactg attaccgtgc tgttctggaa agagtggtcg
    38401 gtatacgcct taattgtcgc gactattatt ggcctgggtg tactggcaaa aatttatcgc
    38461 aaagcagaaa accagaaaca gcggaaggag ctggggctga ttgtgacgct caccttcttc
    38521 agtatgttgt tctgggcctt cgcacaacag ggcggtagct cgattagcct ttatatcgac
    38581 cgcttcgtta accgcgatat gtttggttat accgttccga ccgcgatgtt ccagtcgatt
    38641 aatgccttcg cagttatgct gtgcggtgtg ttcctggcgt gggtggttaa agaaagcgtc
    38701 gcaggtaatc gtaccgtgcg catctgggga aaatttgctc ttggccttgg cctgatgagc
    38761 gccggattct gcattctgac cttaagcgcc cgctggtccg caatgtatgg tcactcttct
    38821 ctgccactga tggtgttagg cctcgcggtg atgggctttg cggaactgtt tatcgacccg
    38881 gttgccatgt cgcaaattac gcgcattgaa attccggacg tgaccggcgt attaaccggc
    38941 atctacatgc tgctttctgg cgcgatcgcg aactatctgg cgggcgtgat tgccgatcag
    39001 acatcgcagg cttcgtttga tgcttccggg gcgatcaact actccatcaa tgcatatatt
    39061 gaagtatttg atcaaattac ctggggcgca ctggcgtgtg taggactggt actgatgatt
    39121 tggctgtatc aggcgctgaa attcagaaac cgcgcgctgg cgctggagtc ataataggcc
    39181 agcaccgatg cctgatgcga cgcttgacgc gtcttatcag gcctacgtat ttcctgcaat
    39241 ttattgaatt tgcacaaatt tgtaggctgg attaggcgtt cacgccgcat ccggcatctg
    39301 ggctcgattg cctgacgcgt ctgttatttc cccttccgcg ccgcctcata cgctgccaac
    39361 gtttgtattc tcgcctcttt gtgctcgact atcggttgcg gataatccag cttcacacct
    39421 gctttctgcg cccacttcca cggctcatgc accgctttcc ctggtacatc gcgcagctcc
    39481 ggtaaccatc gacgaataaa ctcgccctca cggtcaaatt tctcgccctg agttatcgga
    39541 ttgaaaatac gaaaatacgg cgcagcatca gtacccgtag aagccgccca ctgccagcca
    39601 ccgttattag ctgccaaatc accatcaatc agctgcgaca tgaaatatcg ctcgccttcg
    39661 cgccagtcga tcaacaaatc tttcaccaga aaactggctg taatcatccg tagccggtta
    39721 tgcatccagc cagtgctgtt aagctgacgc atagcggcat caataatcgg gtatcccgtt
    39781 ttgcctttct gccaggcctg taaatgtgcg ggattgcttt gccactgcac acgatccgtc
    39841 caggcaataa atggacgatg tttacacaac gaggggtaat acgtcatcag atgacggtag
    39901 aactcgcgcc agatcagctc actaagccag acactaccgg ccccaccgtc cagcgcctgc
    39961 ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg ataacccgcc cgttgccaag
    40021 ctggcggaca aacggctggt gccttccact gccggaaaat ctcgttgttg ctcatactct
    40081 ccagcaccgt tctcgcaaaa ctggcgtaat tgcgcaatcg ccgctttttc ttccaccggg
    40141 aaatgcgcag tatcgaaaga ctgacgagga taattcagcg taatggatgg cgcgggcttt
    40201 atcgatccgc tactacgaac ttttggtgca gcgacgcact ccggcatccc ttcccgcagc
    40261 cgtttcagcc aggcattctt aaaaggcgta aagactttgt acatctcgtg attgccggtc
    40321 atcaccgccc ccggcggcag gatcacgctg tcatcaaatc cttcacacac cacgttacgc
    40381 agcgctcttt caacgtccac atcccgcgct cgctcattca cctcatactg atagttataa
    40441 aaaagatgag taacgctgtt ttccgcgcac acctgtttaa ctatttcgac actggcgaca
    40501 aagtcatcca cttcacggaa caataaagga ataccttttt ccgccagcgc gatttgtagc
    40561 ccattcagtt gagcattgat aagttcagcc tgacgcggcg acatgttatg cgtctcccac
    40621 tggcgtggtg tagcgatata cagcgccagc acgc
//
LOCUS       DADLSA010000020        40184 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.020, whole genome shotgun sequence.
ACCESSION   DADLSA010000020 DADLSA010000000
VERSION     DADLSA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.020"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(1..523)
                     /locus_tag="JN691_001083"
                     /pseudo
     CDS             complement(1..523)
                     /locus_tag="JN691_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiE"
     gene            complement(982..1068)
                     /locus_tag="JN691_001084"
     tRNA            complement(982..1068)
                     /locus_tag="JN691_001084"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1032..1034),aa:Leu,seq:gag)
     gene            complement(1083..1415)
                     /gene="secG"
                     /locus_tag="JN691_001085"
     CDS             complement(1083..1415)
                     /gene="secG"
                     /locus_tag="JN691_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAY7242582.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLTTLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1643..2980)
                     /gene="glmM"
                     /locus_tag="JN691_001086"
     CDS             complement(1643..2980)
                     /gene="glmM"
                     /locus_tag="JN691_001086"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAY7242583.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2973..3821)
                     /gene="folP"
                     /locus_tag="JN691_001087"
     CDS             complement(2973..3821)
                     /gene="folP"
                     /locus_tag="JN691_001087"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAY7242584.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3911..5845)
                     /gene="ftsH"
                     /locus_tag="JN691_001088"
     CDS             complement(3911..5845)
                     /gene="ftsH"
                     /locus_tag="JN691_001088"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAY7242585.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMPEQLGDK"
     gene            complement(5945..6574)
                     /gene="rlmE"
                     /locus_tag="JN691_001089"
     CDS             complement(5945..6574)
                     /gene="rlmE"
                     /locus_tag="JN691_001089"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAY7242586.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6700..6993
                     /gene="yhbY"
                     /locus_tag="JN691_001090"
     CDS             6700..6993
                     /gene="yhbY"
                     /locus_tag="JN691_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAY7242587.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(7149..7625)
                     /gene="greA"
                     /locus_tag="JN691_001091"
     CDS             complement(7149..7625)
                     /gene="greA"
                     /locus_tag="JN691_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY7242588.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7873..9306
                     /gene="dacB"
                     /locus_tag="JN691_001092"
     CDS             7873..9306
                     /gene="dacB"
                     /locus_tag="JN691_001092"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY7242589.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(9492..10652)
                     /gene="cgtA"
                     /locus_tag="JN691_001093"
     CDS             complement(9492..10652)
                     /gene="cgtA"
                     /locus_tag="JN691_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAY7242590.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDGEDWDEDDEEGVEFIYKR"
     gene            complement(10668..11633)
                     /locus_tag="JN691_001094"
     CDS             complement(10668..11633)
                     /locus_tag="JN691_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7242591.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11760..12017)
                     /gene="rpmA"
                     /locus_tag="JN691_001095"
     CDS             complement(11760..12017)
                     /gene="rpmA"
                     /locus_tag="JN691_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAY7242592.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(12038..12349)
                     /gene="rplU"
                     /locus_tag="JN691_001096"
     CDS             complement(12038..12349)
                     /gene="rplU"
                     /locus_tag="JN691_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAY7242593.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            12608..13579
                     /gene="ispB"
                     /locus_tag="JN691_001097"
     CDS             12608..13579
                     /gene="ispB"
                     /locus_tag="JN691_001097"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAY7242594.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13808..14086
                     /gene="sfsB"
                     /locus_tag="JN691_001098"
     CDS             13808..14086
                     /gene="sfsB"
                     /locus_tag="JN691_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAY7242595.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(14134..15393)
                     /gene="murA"
                     /locus_tag="JN691_001099"
     CDS             complement(14134..15393)
                     /gene="murA"
                     /locus_tag="JN691_001099"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAY7242596.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(15448..15702)
                     /gene="ibaG"
                     /locus_tag="JN691_001100"
     CDS             complement(15448..15702)
                     /gene="ibaG"
                     /locus_tag="JN691_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAY7242597.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15862..16155)
                     /gene="mlaB"
                     /locus_tag="JN691_001101"
     CDS             complement(15862..16155)
                     /gene="mlaB"
                     /locus_tag="JN691_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAY7242598.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(16155..16790)
                     /gene="mlaC"
                     /locus_tag="JN691_001102"
     CDS             complement(16155..16790)
                     /gene="mlaC"
                     /locus_tag="JN691_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAY7242599.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QVYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16809..17360)
                     /gene="mlaD"
                     /locus_tag="JN691_001103"
     CDS             complement(16809..17360)
                     /gene="mlaD"
                     /locus_tag="JN691_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAY7242600.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(17365..18147)
                     /gene="mlaE"
                     /locus_tag="JN691_001104"
     CDS             complement(17365..18147)
                     /gene="mlaE"
                     /locus_tag="JN691_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAY7242601.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(18155..18964)
                     /gene="mlaF"
                     /locus_tag="JN691_001105"
     CDS             complement(18155..18964)
                     /gene="mlaF"
                     /locus_tag="JN691_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAY7242602.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            19174..20151
                     /locus_tag="JN691_001106"
     CDS             19174..20151
                     /locus_tag="JN691_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAY7242603.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            20165..21151
                     /gene="kdsD"
                     /locus_tag="JN691_001107"
     CDS             20165..21151
                     /gene="kdsD"
                     /locus_tag="JN691_001107"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAY7242604.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRNALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            21172..21738
                     /gene="kdsC"
                     /locus_tag="JN691_001108"
     CDS             21172..21738
                     /gene="kdsC"
                     /locus_tag="JN691_001108"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAY7242605.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            21735..22310
                     /gene="lptC"
                     /locus_tag="JN691_001109"
     CDS             21735..22310
                     /gene="lptC"
                     /locus_tag="JN691_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAY7242606.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            22279..22836
                     /gene="lptA"
                     /locus_tag="JN691_001110"
     CDS             22279..22836
                     /gene="lptA"
                     /locus_tag="JN691_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAY7242607.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22843..23568
                     /gene="lptB"
                     /locus_tag="JN691_001111"
     CDS             22843..23568
                     /gene="lptB"
                     /locus_tag="JN691_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAY7242608.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            23616..25049
                     /gene="rpoN"
                     /locus_tag="JN691_001112"
     CDS             23616..25049
                     /gene="rpoN"
                     /locus_tag="JN691_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAY7242609.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            25072..25359
                     /gene="hpf"
                     /locus_tag="JN691_001113"
     CDS             25072..25359
                     /gene="hpf"
                     /locus_tag="JN691_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAY7242610.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            25477..25968
                     /gene="ptsN"
                     /locus_tag="JN691_001114"
     CDS             25477..25968
                     /gene="ptsN"
                     /locus_tag="JN691_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAY7242611.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTESTP
                     DEA"
     gene            26014..26868
                     /gene="rapZ"
                     /locus_tag="JN691_001115"
     CDS             26014..26868
                     /gene="rapZ"
                     /locus_tag="JN691_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAY7242612.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26865..27137
                     /gene="npr"
                     /locus_tag="JN691_001116"
     CDS             26865..27137
                     /gene="npr"
                     /locus_tag="JN691_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAY7242613.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            27350..27982
                     /gene="yrbL"
                     /locus_tag="JN691_001117"
     CDS             27350..27982
                     /gene="yrbL"
                     /locus_tag="JN691_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAY7242614.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27979..28707)
                     /gene="mtgA"
                     /locus_tag="JN691_001118"
     CDS             complement(27979..28707)
                     /gene="mtgA"
                     /locus_tag="JN691_001118"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HAY7242615.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGSEPFMQQHQLD"
     gene            complement(28704..29357)
                     /gene="elbB"
                     /locus_tag="JN691_001119"
     CDS             complement(28704..29357)
                     /gene="elbB"
                     /locus_tag="JN691_001119"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAY7242616.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKELAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(29587..31923)
                     /gene="arcB"
                     /locus_tag="JN691_001120"
     CDS             complement(29587..31923)
                     /gene="arcB"
                     /locus_tag="JN691_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAY7242617.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(32019..32948)
                     /locus_tag="JN691_001121"
     CDS             complement(32019..32948)
                     /locus_tag="JN691_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAY7242618.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGIRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            33623..38083
                     /gene="gltB"
                     /locus_tag="JN691_001122"
     CDS             33623..38083
                     /gene="gltB"
                     /locus_tag="JN691_001122"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAY7242619.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDMTFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            38096..39514
                     /locus_tag="JN691_001123"
     CDS             38096..39514
                     /locus_tag="JN691_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7242620.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            39698..40177
                     /locus_tag="JN691_001124"
     CDS             39698..40177
                     /locus_tag="JN691_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HAY7242621.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT         9961 a        10847 c         9922 g         9454 t
ORIGIN      
        1 tggactgaac gcttttttat tttttgcatc tttataagca aattgacaag cggaaaataa
       61 actgcccgaa atgacaatca gcaataatat tcctgtcact ttttttgtcg gcaaggaaac
      121 atcatatttt attattactg ccaaaaaaag aagggataaa aaagcaaaag cacatagata
      181 agggatatac atccccagca ttttcacaac ttcatccgga tcactctgca gcacactaat
      241 cgcaaatcca tcattgaaag ttgtaccaaa ggtacaccat gaatagaggc taatactcat
      301 gtctgccgca gtcagtaaca caaatggtgc ggcaacaagc aggcgaagcg gaagggatga
      361 tatacgttta acaaccagga tggtcaggac gaagaacagc aatcgctgaa ccatatgcgc
      421 accggaagag gcgactaagt tcgtaatacc aaaaacaata acacaaagat acaacaacca
      481 atgagattta aaatcttcta gcaaatttgt tgaatactgt catccagaaa tccaagttca
      541 caaccaatat aaacaattat caacaatgtt caattttata tcagacatgt ttttcaactc
      601 tgatccatat cagaccgaaa cactgttagt tttttctttt taaatgatgc gaccattgcg
      661 ttctcagtaa ttaaataaca ttatcaatgc gtctctaaac attacgcaga acgtagccat
      721 cagtagttat actgacagga gcaggatggt ttatcgagaa gtaaacatat ccgtattcgt
      781 accggaactg gagttgacac aataaagtgc caattatgtc agcagtaggg aaaagtttat
      841 aactaaggcg taccggcacc atcgtttcaa ggtaccagct acgagtaaag caactggacg
      901 agatacagat acctgacaac cattcatcag accaggacca aaacgaaaag agacgctttt
      961 cagcgtctct tttctggaat ttggtaccga ggacgggact tgaacccgta agccctattg
     1021 ggcactacca cctcaaggta gcgtgtctac caattccacc acctcggcac ggatactact
     1081 ttttagttcg ggatatcgct ggtcggctta gccggagcag ctggctgagt ttgttcggtt
     1141 ttcgccggtg cactcagatt ttcccattcg ctacctctat tggttttgtt gctattgatg
     1201 ttacccagca ccagactgat gatgaagaat aacgttgtca gcagcgccgt catgcgggtc
     1261 atgaagttac cagaaccact tgaaccaaac agcgtagcgg aagcgcctgc tccgaaggag
     1321 gctcccatat cagcgccttt accttgctgc agcatgatca gaccaacaag gccaattgcc
     1381 acaataagga aaactactaa aagagcttcg tacataatca acctgttcct tgcggagttg
     1441 ccgcgtacca atgcttcaac caataaagcg ggagttttta atctttccca ctgaagcggg
     1501 tgtgaatact aaccaaagcg aatgaccttc gcaagggcaa ttttatcgca ttgtatcaac
     1561 tgcggaaaaa aacagcaaaa ccgatgtgtt cgccgagaaa aaaggcgaca atcgccgcct
     1621 ttttagccag ttatctaacg ctttaaacgg cttttactgc atcggcgatg cggtgtgcaa
     1681 attcagtcac ctgcgcttcg tcttcgcctt ccaccatcac gcgaattaac ggttcggtgc
     1741 cggatttacg cagcaacacg cgtccacggt tgcccagcgc agcttcaacc tctgcggtca
     1801 cggctttaac tgactcatgc tcaagcggat cgccgctacc tgcggtgtaa cgtacgttaa
     1861 ccagaatctg cgggaacatt ttcatgccgc tgcaaaggtc gtgcaggctc atatggttac
     1921 gtgccatcgc cgccagcacc tgcaagccag caacgatgcc gtcaccggta gtggttttat
     1981 ccagcaggat cacatgaccg gaattctctg caccgatacg ccagcctttc tcctgcattt
     2041 tttccagtac gtagcggtca cccactttcg cgcgcgcaaa tggaattcct aactgtttca
     2101 gcgccagttc aagccccatg ttgctcatca atgtacccac agcgccacca cgcagctggc
     2161 cctgacgaag accttcacgc gcgatgatat acatgatctg atcgccatcg actttattgc
     2221 cttcatggtc aaccataatc acgcgatcgc cgtcgccgtc gaaggcaata ccgagatccg
     2281 ctttttcagc cagcacacga gcctggagcg cgcgaacgtc ggtagccccc acttcggcat
     2341 tgatgtttac accgtttggc tcacaaccga tagcgataac gttcgccccc agttcgcgca
     2401 gcacgttcgg cgcgatgtga taagtcgcac cgtttgcaca atccaccaca atcttcagtt
     2461 cactgaggct aagttcgttc gggaacgtgg ctttgcaaaa ctcgatatag cgacccgcgg
     2521 catcaacgat acggctggct ttacccagtt ctgccgaatc aacgcagctg atctcctttt
     2581 ccatttccgc ttcgatggcc tcttctaccg catccggcag tttagtgccg tcgatagaga
     2641 agaatttaat cccattatcg tagaacgggt tatgcgatgc agatatcaca attccggcct
     2701 ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt tggcatcggg ccagtgaaga
     2761 gtgcggaaag gcccgctgcc gccagacccg cttccagtgc tgactccagc atatagccag
     2821 aaatacgcgt gtctttacca ataataatct tacgggagcc gtggcgcgcc agcactttac
     2881 ccgcggccca acccagctta agcacaaaat caggtgtgat cggcgcatcc cctacacgac
     2941 cacgaatccc atcggtaccg aaatatttac gattactcat agcgtttgtt ttcctttgca
     3001 gacagagtgg cttccaccac ccgcatcgct tctacggttt ctttgacgtc atgaacacga
     3061 atgatgtgcg cgccttgcat tgcggcaatg accgcacagg ccagactacc gctcaggcgc
     3121 tcggacggcc ccacgttcag cagctgccca atcatcgatt ttcgtgacat acccaccaac
     3181 agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca gtaatgaata gttatgggag
     3241 agatttttac cgaaaccgaa tccggggtcg agcaacaatt tctcttttgc gatacccgcc
     3301 tgctcgcaac gtgctatttg ctcaataaag tagcgattca cttctgcaaa gacatcgtca
     3361 tacttcggag cttcctgcat ggtttttgga tttccctgca tatgcatcag acaaaccggt
     3421 aaaccggttt ctgcagccgc ctccagagcg ccaggttcgg aaagggagcg gatatcatta
     3481 ataatgtgag cgccaacttt cgctgactca cggatgactt ctggtttgga tgtatcgacc
     3541 gagatccaga cttcgaagcg ttgagcaatt gcctcaacca caggaataac acgttgcaac
     3601 tcttcttcaa cgctaacttc cgccgcccct gggcgcgtgg actcgccacc aacgtcaatg
     3661 atcgtcgcgc cagcattgat catcagattc gcatgtttca ccgcatctat cagcgagtta
     3721 tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga ggatccccat tacgtgagga
     3781 tggctaaggt ccagtgaagt accctgggca aagagtttca tgatgttatc cctggtatga
     3841 attgataaga aaaaaccccg gagcacgccc cggggttttc ggtacaaata cagtcatctg
     3901 atgcgggaac ttacttgtcg cctaactgct ctggcatggt gttacccggg ttcggcgtac
     3961 gcggttcatc aaccggacga ggagcctttg gactaccatt gtcgccagaa ttgttagaag
     4021 cgcctggttc ttcccagccc gctggcggac gtacatcgcg acgtgccatc aggtcatcaa
     4081 tctgcggtgc gtcgatagtc tcatatttca tgagagcatc tttcatcgca tgcagaatat
     4141 ccatattgtc ggtcagaagc tgacgcgcac gattatagtt acgctcaatc agtgctttca
     4201 cttcctggtc gatgatacgt gcagtttcat cggacatatg tttcgctttc gctacgctac
     4261 ggccgaggaa tacttcacct tcttcttccg cgtacagcag tggacccaat ttctcagaga
     4321 agccccactg agtcaccatg ttacgtgcca ggttggtcgc aactttaata tcgttggacg
     4381 caccggtaga aacatgttcc ggcccgtaga tgatctcttc tgccagacga ccaccgtaca
     4441 gcgtagaaat ctggctttcc agtttctgac ggctggcgct gattgcgtcg ccctcaggca
     4501 agaagaaagt cacacccagc gcacgaccgc gtgggataat cgtcacttta tgcaccggat
     4561 cgtgttccgg caccaggcga ccgataatcg catgacccgc ttcgtggtaa gccgttgatt
     4621 ctttctgcgc ttccgtcatc accatggagc gacgttccgc acccatcatg attttgtctt
     4681 tcgctttctc gaactcaacc atcgacacaa cgcgtttgtt gccacgagca gcgaacagtg
     4741 ccgcttcgtt caccaggttc gctaggtcag caccggagaa accaggagta ccacgggcaa
     4801 tgattgccgc gtcgatatcg ggtgccaatg gtacgcgacg catgtgaact ttcaggatct
     4861 gctcacgacc gcgaacatct ggcaagccga ccacaacctg gcggtcgaaa cggccaggac
     4921 gcagcagggc cgggtcgaga acgtccggac ggttagtcgc ggcgataacg atgatacctt
     4981 cgttaccttc gaagccatcc atctcaacca gcatctggtt cagagtctgt tcacgttcat
     5041 cgtgaccacc gcccagacca gcgccacgct ggcggcctac ggcgtcgatt tcatcgataa
     5101 agatgatgca cggtgccgct ttcttcgcct gttcgaacat gtcacgaaca cgggatgcac
     5161 ccacaccgac gaacatttct acgaagtcag aaccggagat agtaaagaac ggaactttcg
     5221 cttcgcctgc aatcgctttc gccagcaacg ttttaccggt acccggagga ccgaccatca
     5281 ggacgccttt cgggatctta ccgccgagtt tctggaagcg gctcggctcg cgcagatact
     5341 ctaccagttc agcaacttct tcttttgctt cgtcgcagcc cgcaacgtca gcaaaggtcg
     5401 ttttgatctg atcttctgtc agcatgcgcg ctttgctctt accaaacgac atggcacctt
     5461 tgccaccgcc gccctgcatt tgacgcatga agaagatcca gacaccaatc agcaacagca
     5521 tcgggaacca ggagatgaag atagaagcca gcaggcttgg ttcttcaggc ggttcaccga
     5581 caaccttgac gttcttggtc aacaggttat ccagtaattt cggatcctga accggaatgt
     5641 aagtggtata acggttacta tctttcttgg taacgttgat ttcacgtccg ttgatacgcg
     5701 cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt agagtaatcc accttacggc
     5761 cattagactc gctgggccca aagctctgga atactgacat cagcacaacg gcaatgacca
     5821 gccagagtat taggtttttc gccatgtcac tcaagggatt aacctcttat tacaactgtg
     5881 ttaaaaacag cgtcaggata ctctatatcc agcatctttc aaactttcgt ctgaaatctc
     5941 ccggttaggg tttacgcccg gtcgctacaa tatacacttc tcgcgaacgt gcacgagaag
     6001 agtccggctt acgaactttg accttcgtaa acagggagcg aatttccctt agatactcat
     6061 cgaaaccttc gccctggaac accttcacta caaaactgcc acctggcgct aatacatcac
     6121 gacacatttc tagcgccagt tccaccagat acatggcacg ggggatatcc accgccggtg
     6181 ttccgctcat gtttggtgcc atatcagaca tgacaacctg gactttgctg tcgccaacgc
     6241 gctccagcag tgctttcatc accagttcat cacgaaaatc gccctgaaga aagtccacac
     6301 caacgatagg atccataggt aaaagatcgc aagcgatgat gcggcctttg ccgccaattt
     6361 gggtgaccac atattgtgac caaccacccg gagcagcacc aaggtcgaca accgtcattc
     6421 ccggtttaaa gagtttgtca ctttgctgta tttcatcaag tttaaaccag gcacgggaac
     6481 gtaatccctt tttctgtgcc tgttgaacat atttatcgct aaagtgttcc tgaagccagc
     6541 ggctggagct ggcagaacgc ttcttacctg tcatttaact ttcccatggg gataactcat
     6601 cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga tatatgggag atggcggtag
     6661 aatgacccgt tttcaatccc aacgtaagca aaaaatacga tgaatctgag tactaaacaa
     6721 aaacagcacc tgaaaggtct ggcacatccg ctcaagccag ttgttctgct tggcagtaat
     6781 ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt tagagcacca tgaactcatc
     6841 aaggtgaaaa tcgccaccga agatcgcgaa acaaaaacct tgatcgtgga agctatcgtg
     6901 cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa cgctggtgct ttatcgccca
     6961 actaaagaac gtaaaatctc gctgccacgc taagattatc ctaaagttac acacatttgc
     7021 tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa tgccacgctc tgttcgttga
     7081 taaaaggccg cagagcggcc tttttccttt ctttacaata catcaacatc ttgagtattg
     7141 ggtaattctt acaggtattc caccttaatt acttcaaatt ctacttcgcc gcccggcgtt
     7201 ttgatgacca caacatcatc ttcttctttg ccgatcaggc cacgagcaat aggcgagttt
     7261 acagaaatca ggttttgttt aaagtcagct tcgtcatcgc caacgatgcg ataagtctgt
     7321 tcttcgtcag aatccagatt cagcaccgtt acggtagcac caaaaataac gcgcccattg
     7381 ttgggcattt tggtgacatc aatcacctgc gcgtttgaca gcttggcttc gatgtctttg
     7441 atacggcctt cgcagaagcc ctgctgttca cgagctgcgt ggtattcggc gttttctttc
     7501 aggtcgccat gctcacgcgc ttccgcgata gcagcaatga tttcaggacg gcgcacagat
     7561 ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc gtaaggtcat cggaatagct
     7621 tgcatttgtt atacctcttg aatattcctg atggggcaag tcttcaccca ccccggctgt
     7681 tcggcctgcc cggcataact cctcaccggg accagaagca aaaaaatacc gacccgggta
     7741 caagtcccag gtcagctaca attcacattt tgatggtcat tttaccctga agttcccgaa
     7801 gggtcatcgt ttactttata gggcgttgcg ccgtagtatg acggctcgat tccaggttgt
     7861 tagcgcgaga ttatgcgatt ttccagattt atcatcggat tgaccagctg tatagcgttc
     7921 agtgttcagg ccgcgaatgt tgatgagtac gttactcaac tccccgcagg tgccaacctt
     7981 gccctgatgg tgcaaaaagt cggtgcgtcg gcccccgcta ttgattacca cagtcagcag
     8041 atggcgctgc ccgccagtac ccagaaagtg attactgcgc tggcggcgtt gattcaactc
     8101 ggccccgatt ttcgtttcac cacgacgctt gaaaccaaag gcaatgtgga aaacggcgta
     8161 cttaagggtg acttagtggc gcgatttggt gccgatccga cgttaaaacg tcaggatatt
     8221 cgcaatatgg tcgcgacttt gaaaaaatct ggcgtcaacc aaatcgatgg caatgtgttg
     8281 atagatacct ccattttcgc cagccacgat aaagcccccg gctggccatg gaatgacatg
     8341 acacaatgct ttagcgctcc gcctgctgcc gccatagttg accgcaactg tttctccgtc
     8401 tcgctctaca gtgccccaaa gcctggtgat atggctttta tacgcgtggc atcttattac
     8461 cccgttacga tgttcagcca ggtacgcacc ctcccccgtg gttctgccga agcgcaatac
     8521 tgcgaactgg atgtggtgcc aggcgacctg aaccgcttta cgctgacggg atgcctgcca
     8581 caacgttctg agccgctccc gttggctttt gccgtgcagg atggagccag ctatgccggt
     8641 gcaattctga aagatgagtt aaaacaggcg ggtatcacct ggagcggaac actgctgcgc
     8701 cagactcagg ttaacgaacc tggaacggta gttgccagta aacagtcggc cccgctgcac
     8761 gatctgctta agattatgct gaaaaagtcg gacaacatga tcgccgatac ggttttccgc
     8821 atgatcggcc atgcgcgctt caatgtgcct ggaacatggc gggctgggtc ggacgccgtg
     8881 cgtcagatcc tgcgccagca agccggtgtc gatattggta acaccattat tgccgatggt
     8941 tcagggcttt cgcggcataa cctgattgcc cccgccacca tgatgcaggt gctgcaatac
     9001 attgcccaac acgacaatga acttaacttt atctccatgc tgccgcttgc gggctatgac
     9061 ggctctttgc agtaccgtgc aggtctgcat caggcgggcg tggatggcaa agtctcagcg
     9121 aaaaccggtt cgttgcaggg ggtatataac ctggcgggat tcattaccac agcgagcggg
     9181 caacgaatgg catttgtaca atatctttct ggctatgcag tagaacccgc ggatcagcgt
     9241 aatcgccgta ttccgttagt gcgttttgaa agccgtttgt acaaagatat ttatcagaac
     9301 aattagtcaa aaagaaaccc cggcgcatgg ccggggtttc agattattga caaagtgcgc
     9361 tttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaatcg tgcaaattca
     9421 atatattgca atcatcttgt aggcctgata agtgtagcgc atcaggctta tttggcattg
     9481 gtcattagtg attaacgctt gtaaatgaac tcaacgcctt cttcgtcgtc ttcgtcccag
     9541 tcttcaccat cctcttcagc aatctcttca agctgctgac ggtgataatc atcccacatg
     9601 aattcgactt tctctggctg tttcgcttct tcagcctgca cgaccgggtt ttcaatgata
     9661 aaggtcatca catcccagca gagatctttc acgcccagtc cgctcgccgc agagatcaga
     9721 taatatttat cttcccagcc cagcgcttca gcgatcgctt tcgctttctc ttcggcttct
     9781 gccttatcca gcagatcgat cttgttgaag actaaccaac gcggtttcgc cgccagatcc
     9841 tggctgtatt tttccagctc gctgataata atacgcgcgt tttcaaccgg atcggtgccg
     9901 tcaatcggat cgatatcgat gaggtgcaac aggacgcggc aacgttccag gtgcttcagg
     9961 aagcgaatgc ccagacctgc gccttccgca gcgccttcaa tcagtcctgg aatatcggca
    10021 acaacgaagc tcttttcgtt gtccattcgt accacaccca gacttggcac cagagtggta
    10081 aacggataat ccgccacttt cggtttagcc gccgataccg cacgaataaa ggtcgattta
    10141 cccgcgtttg gcatccccaa cataccgacg tcagccagca gcatcagctc cagcagcagc
    10201 tcacgcttat cgcccggcgt accgttagtt ttctgccgtg gtgtacggtt aactgacgat
    10261 ttgaaacggg tattgcccag accgtgccag ccgcccttag caaccagcag acgctgaccg
    10321 tgtttagtca tatcgcccat ggtttcacca gtaccctggt cgattacacg cgtacctacc
    10381 ggcactttaa tcgtcacgtc tttaccgcgc ttaccggtac aatcgcggct tgcgccgttc
    10441 tgaccgcgct ctgcacggaa agatttttca aaacgataat cgataagcgt gttcaggttc
    10501 tcgtcggctt ccatccatac gtcaccacca tcaccgccgt cgccgccatc cgggccgcct
    10561 ttcggaatat acttttcgcg gcggaagctc acgcaaccat taccgccatc acctgcaacg
    10621 accagaatcg atgcttcatc aacaaacttc attttattct ccgtaaatca ttcgcctgcg
    10681 cggggttgcg ataccaccgt tgtatgctta cgtaatccgc cccaaatacg atgaccaatg
    10741 gcggaataca tcgcgcccgc aaccacgaca aacgcaccga gataacctaa aaggtttaac
    10801 atcggtctgg cgaagaaatc gggccaggcc agtgataaaa gatctgaaaa aaacagcgta
    10861 aacagtgggg tgagcgtgat gatcgcgctc acctgcgctg cctgccagcg agccatcgct
    10921 tccgccaggg cgccatatcc taccaaggta ttcagtccgc aaaaaattaa acatgcgagc
    10981 tgccagtggc taagctgcgc tatcactcca ggctttgcca gagggaagag cgcgattgta
    11041 cataaagtgt acagtaaaaa caggatctgc ggtgaggcca gccgacgcaa taaaaccttt
    11101 tgcgccacgc cataactcac ccaaaccgtc gccgcaccga ccccaaagat aactccccag
    11161 gtgtaatcgg tgagctttgt aaatatctcg accagactgg tgttaaaaaa catcaccaga
    11221 ccgctcagga gcatcaatgc ccctacaacc tgagtgctgc gcattttctc tttcaggata
    11281 aatacgctgg caaccatcat gccaactggc gagagttgcc caatcacctg cgaagcggtc
    11341 ggactcaggt attgcaagga tgagctgaac aggatgaagt tcccaaacag cccggcggtc
    11401 gccactgcca acaaaatcaa ccagcgtggc ttacgaaaca cgcgtaatgg cggcaacctc
    11461 ttcttcaccg caagaatggc accaaggcca atactcgcca tcaagaaacg gtaaaacacg
    11521 attgtcggag gttccatcac ctccagcacc tgcttcattg cgattggcaa cgccccccag
    11581 caaattgctg tggtaagcgc caaaagaatg ccaatgcctg cctgctgctt cataccgtat
    11641 tccctgcaaa gcgcatttgc ggattaacgt cgcaccatgc gcatttttac cggttatcga
    11701 atgtaaaaag ccccgcaacg tgttgcgggg ctttcatccg ttaccgggac gcgaaaaact
    11761 tattcagctt cgatgctgat aaatttacgg tttttcgggc ctttaacttc gaatttcact
    11821 ttaccgtctg ctttagcaaa cagagtgtgg tcacgaccgc aacctacgtt agcgccagcg
    11881 tggaatttgg taccacgttg acgaacgatg atgctacccg ccagaacgga ttcgccaccg
    11941 aaacgcttaa cgcccaggcg tttagcttct gaatcgcgac cgttacgtgt ggagccgcca
    12001 gcctttttat gtgccatttg aaatctctcc tcaggtctta ggcgctgatg ccagtaattt
    12061 tcacatcagt gaaccactga cgatggccct gctgcttacg atagtgttta cgacgacgaa
    12121 acttaacgat tttaactttc tcgccacgac cgtgagcaac aacttcagct ttgattacgc
    12181 cgccatcaac gaaaggaacg ccgattttga cttcttcacc gtttgcgatc atcagcactt
    12241 cagcgaactc aacagtttcg ccagttgcga tgtccagctt ttccaggcga acggtctgac
    12301 cttcgcttac tcggtgttgt ttaccaccac tttggaaaac cgcgtacata taaaactccg
    12361 cttctgcgca caccttttca atgattcaga gtgcgctata aatattcaca atagggcgcg
    12421 aatattacgc aaaacgcacg cctttgacaa gtgctacagt caatacacga agaaaaaaaa
    12481 cacaacttgt acggtaacgt ttatctgtgc cattttttca gtacaattac cctatattcc
    12541 taaccataaa ccctaagttg cctttgttca cagtaaggta atcggggcga aaagcccggc
    12601 ttttgcgatg aatttagaaa aaatcaatga gttaaccgcg caagatatgg cgggtgttaa
    12661 tgcggcaatc cttgagcagc ttaattccga cgtccaactg atcaatcagt taggctatta
    12721 catcgtcagc ggcggcggta aacgtattcg tccgatgatt gctgtactgg ctgcacgagc
    12781 tgttggctat gagggaaatg cgcatgtcac cattgctgcc ctgatcgagt ttatccacac
    12841 ggcgactctg ctacacgacg acgttgtgga tgaatcagat atgcgcaggg gtaaagctac
    12901 cgccaacgcc gcatttggca atgccgccag cgtgctggta ggcgatttta tttatacccg
    12961 cgcgttccag atgatgacca gcctcggttc actcaaagtg ctggaagtca tgtcagaagc
    13021 tgtaaacgtc atcgcagaag gtgaagttct gcaactgatg aacgttaacg atccggacat
    13081 cactgaagaa aactacatgc gcgttatcta tagcaaaacc gcgcgtctgt ttgaggctgc
    13141 cgcgcagtgt tccgggattc tggctggctg tacgccggag gaggagaaag gcctgcagga
    13201 ttatgggcgc tatctcggca ctgctttcca gttgatcgac gatttactcg attacaatgc
    13261 cgatggcgaa cagttaggta aaaacgtcgg cgacgatctg aacgaaggta aaccgacgct
    13321 gccgctgctg catgcgatgc atcatggcac accagaacag gcacagatga tccgtaccgc
    13381 catcgaacag ggtaacggtc gccatcttct ggaaccggtt ctggaagcaa tgaacgcttg
    13441 tggatctctt gaatggacgc gtcagcgcgc cgaggaagaa gcagacaaag ccatcgcagc
    13501 gttacaggtg ctcccggaca ccccttggcg agaagcactc atcggcctcg cgcacatcgc
    13561 tgttcaacgc gatcgttaat cccctccctt catcccgcgc agtgcgcggg atgattccag
    13621 taaattccat aaaaacacta attcagcgct ataacacctg aaaaaaaagg gcgatgaatc
    13681 tttatatatt ctgaatattc acactcttta caggaacttt ttagcgcaat aggccatcag
    13741 gagtatagtg atgcccgaca gaagaagtgt tctgaatgaa agcgactaac ttaaggagtg
    13801 aggaaagatg gaaagtaatt tcattgactg gcatcccgct gacatcattg caggtttgcg
    13861 caagaaagga acgtcaatgg cggcggaatc tcgcagaaat ggtttgagtt cctcaacgct
    13921 ggcaaacgca ttatcgcgcc catggccgaa aggagagatg attattgcga aagccctggg
    13981 aactgacccc tgggttatct ggccatcacg ctaccatgat ccgcagaccc atgagtttat
    14041 cgacagaacg cagttgatgc gtagctacac taaaccgaaa aaatgagtgg tctggcggta
    14101 gccccgcgaa cggggctgcc agctctcaga cgattactcg cctttcacac gctcaatatt
    14161 tgcacctaaa gcgcgcagtt tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat
    14221 acgatcaacc accgtcgtcc cttccgcaat acagccagcc agcaccaggc ttgctgatgc
    14281 acgcagatcg gttgccataa cctgtgcacc agaaagtttt tcaacaccat gacaaataac
    14341 ggtattgctt tcgatttcgg cgtgcgcgcc catacggctc agctctggca catgcataaa
    14401 gcggttttca aagaccgttt cggtgatgaa cccggtccct tcagccacca ggttcaacag
    14461 cgtgaactgg gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac
    14521 agccttcgga cgtttgccat gcatatccag gctaatccag tcttcgccga cttcgatgtc
    14581 cgctccagca tcacgcagtt tcgccagcac ggcgtccaga gtatctggct gcgcgttacg
    14641 gcagataatt ttgccacgag agatcgccgc cgccaccagg aaagtaccgg tttcgatacg
    14701 atccggcagc acgcgataga caccgccgcc taaacgttcc acaccttcga tgacgatacg
    14761 atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac
    14821 gatttccggt tcacgcgctg cgttttcaat aatcgtggtg ccttccgcca gggttgcagc
    14881 acacatgatg gtcaccgttg cgccaacgct gactttatcc atcacgatat gtgcgccttt
    14941 cagacgacca tcgacggaag ctttaacgta accttcttcc agtttgatgg tcgcgcctaa
    15001 ttgttcgagg ccagaaatgt gtagatcaac cggacgcgca ccgatcgtac aaccgccagg
    15061 tagtgaaact tgcccctgac caaagcgcgc taccagcggc cccagcgccc agatagaagc
    15121 acgcatggtt ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat
    15181 atgcacagaa ccattacgtt ctactttcgc acccagctgg cttagcagtt tcattgatgt
    15241 atcgacgtct ttcagtttcg ggacgttctg gatctctacc ggttcttccg ccagtagtgc
    15301 ggcaaaaaga ataggcagag cagcattttt agcgccggaa attgtgactt cgccctggag
    15361 cttcgttggc ccctgaacac gaaatttatc catttagttt gttctcagtt aacaattcat
    15421 atccgctacc ggcgaatcgc ccatagctca aaagccgttc agtttgcgat cgcgcgccca
    15481 ctccgcaggg gtatacgctt tgatcgacac agcatgaatg cggttatccg caatatattc
    15541 catcagcggg ccatagaccg tctgctgttt tttaacccga ctcataccgt caaacaactc
    15601 acccacggca ataacctgaa agtggctgcc atcgccggaa acgtggactt cctggaggga
    15661 gagagcgttc atcaacacgc tctgaatttc attattttcc atgggatctt caatcatcag
    15721 ttaataaacc agcgaaacat cttagagcaa agttgcgctg gcataaataa gcaaaaagcc
    15781 tcgctgataa atcagacaag gctcgacttg caggcaggtt tgccggacag gcggttaacg
    15841 ccatatccgg cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat tatacaattt
    15901 cgcgagggta tacactttgt cgtttacccc ctgaagcgtc acgttgttgc cctgcttttt
    15961 cgccagatcg ataagatgga gcagcagtgc cagtcccccc gtatccacgc gggacacacg
    16021 gctaagatcg atgcaggtaa tccccttcac cgcttcctca cgcatttccc aaagcggtag
    16081 cagaacgtcc tgatccagct ctccggataa cgccagcgtg tcacccgtct gcatccagct
    16141 cagtgactcg ctcattattt tttctcttcc agagtgattt tctgttgaga aatcgatttc
    16201 agttgcgcag tcaggccgtc gatacctttg gtacgcagca gcgttcccca ctcgttttgt
    16261 ttggtggtga tcatactgac gccttcagca atcatgtcgt aagcttgcca gtttcccgtc
    16321 tgggagtttt tacgccactg gaagtccaga cgcaccggcg gacggccatt cgggtcaata
    16381 atggtaacgc gaataggcac aatggtttta tcgcccagcg gctgttctgg cgcaatctga
    16441 taggtttgac cgtgatacat cgccagcgcc tgaccgtaaa cctgcttcag gtactcacgg
    16501 aaagcggcaa agtaggcttc acgttgagca ggggtcgcac tcttgtaata ctggcccagc
    16561 accagcgcac cggcgtattt cacctgtacg tatggcagca gttcctgatc aacaatggta
    16621 cgcagataat ccgggttggc ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt
    16681 ttctgcgccg cctcgtccat cagcttataa ggattggtct ggtctgccgc ggttgccgca
    16741 ctcagaggtg caatcaccag caaagcgacc atcattaaac gtttaaacat gcgtcggttc
    16801 tcctgaaatt atttcgttgt acccacaggt tcagtggttt cattattacc tggcgcagca
    16861 gctggcgcat cgccactatt cttattgtca tcgcctttac taccgtaaag gaactgacca
    16921 atgagatctt ccagcaccat cgcagacttg gtgtcctgaa ttgtatcgcc atccttcagg
    16981 atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta atgccagata ttgttctccc
    17041 agcaggccgg aagtacgaat gctcagcgaa ctggtatcag gaatgtggtt ataacgttgt
    17101 tcaatttcca gcgttacgcg cggcagatag gttttcgggt ccagcgtaat atccgccacc
    17161 cggcccacaa caacaccacc aatgctgacc ggagagcgcg ctttcaggcc gccaatgtta
    17221 tcgaacgtcg cataaagcgt gtaggtcggt tcagtacgta tggacgtcac gttcgccgcc
    17281 ttcaggcaaa caaacagcgc cgccagcagt gctgctaata aaaagatacc cacccaaatt
    17341 tcattttttt tcgtttgcat gaactcaatt cccaaacatc aatgcggtca gcacaaaatc
    17401 cagccccaga acagccagag acgagtggac aacggtgcga gtggttgccc ggctaatccc
    17461 ggcagacgtc gggatggcgt cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc
    17521 gaacaccacg ctcttaatca gacagttgac cagatccata cgccagtcga cggcattttg
    17581 cattgccgac cagaagaacc cgctatcaat gcctttccaa ctgacgccga ccagcgatcc
    17641 gccccagatc cccacggcga cgaagataac cgtcaacagt ggtaatgaaa taaccccagc
    17701 ccagaaacgg ggagaaataa cccgacgcag cggatccacc gccatcatct ccatactgga
    17761 gagttgctct gtagcgcgca tcaggccgat ttctgcggtt aacgccgaac cagcacgccc
    17821 ggcaaacaac agcgcggcaa ccaccggccc cagttcacgc agtagcgata acgccaccag
    17881 catacccaga ctggtttccg cgctataagt ggtcagaacc agataacctt gcagccccaa
    17941 caccattccg atgaacacgc cagaaactac aataatcagc atcgacagga cgccgacatt
    18001 atagagctgg cgcaccagca gcggcgcatg tttgcgaaat tccggtttgc cgaccagcgc
    18061 attgaataac attaacccgg cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc
    18121 gagcgacgcc agcgcattta acagcatgag tggcttaact ccctggtaaa agatcagcgt
    18181 gataatcgcc ggcaggatag cggaacggaa caggcccgtc agctatcccg tccagaaact
    18241 gacgtacgcg cggatcagga ttcgtctgca acgcctgggc actgccatga gcgacaattt
    18301 ttttgtccgc caggatccag gcgtgatccg caatacttaa cacttccggc acatcgtgag
    18361 aaaccaccac acaagtcacg cccagcgcgc tgttcagctc agaaatcagc ttcaccagta
    18421 cgcccatggt gatgggatct tgcccaacaa aaggttcatc aaacatgatg agatccggct
    18481 ccagcgcaat cgcacgcgcc agcgctgcac gccgcgccat cccaccggaa agttcagaag
    18541 gcattagttt agccgctcca cgcagcccca cggcctccag cttcatcatc accgtactat
    18601 gcaacaatgg cgcgggaagt tgggtatgtt cgcgcagtgg ataggcgacg ttgtcaaata
    18661 cgttcatatc agtgaacaac gccccggact gaaataacat gctcatccgt ttacgcactg
    18721 tatacaggcg cgaacgagac atcgccggaa tattctcacc atcgaaaagg atctcaccat
    18781 gatctggtgc gatttgcccg ccaatcagac ggagtagcgt cgttttaccg atgcccgatg
    18841 gccccatgat cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc
    18901 agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc acagactgct
    18961 ccataattca cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga
    19021 acccaacata tttacagaat attacccgcc gtggttagcg aaagctggca tttgttttac
    19081 tttttagccg cataaagtca aaattaagaa tccgttacgg ctttctgaaa atcttcagcg
    19141 gaccggcgag tatacctgaa gaaaggacgt tagatgcttt tagctacggc actgttaatt
    19201 gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc gtctattctt
    19261 tgccgaacct ttggcatccc gccgctgatc atcggcatga cggtggtcag tattggtaca
    19321 tcgttaccag aaatcatcgt ctcgcttgcc gcgtctctgc acgaacaacg cgatttagcc
    19381 gttggtacag ccctcggctc aaacattatc aatatattgc tgatcctcgg tctggctgcg
    19441 ctggttcgtc cttttaccgt ccattccgat gttctgcgcc gtgaattacc cttaatgttg
    19501 ttggtcagcg tggtggccgg ttccgtactc tatgacggac aacttagtcg aagcgatggt
    19561 atctttctcc tctttctggc tgtgctatgg ctgctgttca ttgttaaact tgcacgtcag
    19621 gctgaacgtc aggggactga cagcctgacc agagagcagc ttgcagagct gccgcgtgac
    19681 ggcggattgc ccgtcgcgtt tttatggctc ggcattgcgc ttatcatcat gccagtggcc
    19741 acgcggatgg tggttgataa cgccacggtg ctggcgaatt actttgccat cagcgagttg
    19801 acgatgggtc tgacggcaat tgctatcgga accagtctgc cggaactggc aaccgcaata
    19861 gcgggggttc gcaaaggtga aaacgacatt gctgtcggaa atatcattgg cgcaaacatt
    19921 tttaatattg tcatcgtgtt gggtttaccc gcgctgataa cgccaggaga gattgatcca
    19981 ctggcgtaca gtcgtgacta cagcgtgatg ttgctggtga gcattatttt tgcgttgctg
    20041 tgctggcggc gctccccgca accgggccgt ggtgtagggg tattattaac tggcggattt
    20101 atcgtatggc tggcgatgtt gtactggtta tcgccaatac tcgttgaata actggaaacg
    20161 cattatgtcg cacgtagagt tacaaccggg ttttgacttt cagcaagcag gtaaagaagt
    20221 cctggcgatt gaacgtgaat gcctggcgga gcttgatcaa tacatcaatc agaatttcac
    20281 gcttgcctgt gaaaagatgt tctggtgtaa agggaaagtt gtcgtcatgg ggatggggaa
    20341 atcggggcac atcgggcgca aaatggccgc aacgtttgcc agcaccggta caccttcatt
    20401 tttcgtccat cctggtgaag ccgcgcatgg tgatttaggc atggtcaccc cacaggatgt
    20461 ggtgattgct atctctaact caggtgaatc cagcgaaatc acggccttaa ttccagtgct
    20521 taagcgtctt cacgtaccgt taatctgcat caccggtcgc ccggagagca gcatggcgcg
    20581 cgccgcagat gtgcatctgt gtgttaaagt agcgaaagaa gcctgtccgt tagggctggc
    20641 accgaccagc agcaccaccg ccacgctggt tatgggcgat gccctcgctg tcgcgctgtt
    20701 aaaagcacgc ggctttactg ctgaagattt tgcgctctca cacccaggcg gcgcactggg
    20761 tcgtaaactt ctgctgcgcg taaacgatat tatgcatacg ggcgatgaga tcccgcatgt
    20821 taagaaaacg gccagtctgc gtaacgcatt gctggaagtt acccgcaaaa atcttggtat
    20881 gactgtcatt tgcgatgaca atatgatgat tgaaggcatc tttaccgacg gtgatttacg
    20941 ccgtgtcttc gatatgggcg tggatgttcg tcagttaagt attgccgatg tgatgacgcc
    21001 ggggggaata cgtgtacgcc ctggcattct ggccgttgag gcactgaact taatgcagtc
    21061 ccgccatatc acctccgtga tggttgccga tggcgaccat ttactcggtg tgttacatat
    21121 gcatgattta ctgcgtgcag gcgtagtgta aagattcaag gataaacaac aatgagcaaa
    21181 gcaggtgcgt cgcttgcgac ctgttacggc cctgtcagcg ccgacgttat ggcaaaagca
    21241 gagaacattc gtctgctgat cctcgacgtt gatggcgtac tgtcagatgg cctgatttat
    21301 atgggcaata acggcgaaga gctgaaagcg ttcaatgttc gtgacggtta tggcattcgt
    21361 tgtgcgctca cctctgatat tgaagtcgct atcattaccg ggcgaaaggc taaactggta
    21421 gaagatcgtt gtgccacatt ggggatcact cacttgtatc aggggcagtc aaacaaactg
    21481 atcgccttta gcgatttgct ggaaaaattg gcgattgccc cggaaaatgt ggcttatgtt
    21541 ggcgatgatc tcatcgactg gccggtaatg gaaaaagtgg gtttaagcgt cgccgtggcc
    21601 gatgcgcatc cactgttgat cccgcgcgcc gattacgtga cgcgcattgc tggcggtcgt
    21661 ggcgcagtgc gcgaagtttg cgacttatta ctcctggcgc agggcaaact ggatgaagcc
    21721 aaagggcaat cgatatgagt aaagccagac gttgggttat cattgtgcta tcactggcgg
    21781 ttctggtgat gatcggcatt aatatggccg aaaaagacga taccgcccag gtggtcgtca
    21841 acaacaatga tcccacctat aaaagcgagc atacggacac gctcgtctat aacccagaag
    21901 gggcactaag ctatcgattg attgctcaac acgttgaata ttattccgat caggccgttt
    21961 cgtggtttac gcagccggta cttaccacgt ttgataagga taaaatcccg acatggtccg
    22021 taaaagcaga taaagccaag ctgaccaatg accggatgct ctatttatat ggacacgttg
    22081 aagtcaacgc acttgtgcca gactctcaac ttcgcagaat cacgacggat aacgcgcaga
    22141 tcaatctggt gacacaggat gttacctctg aagacctcgt cacgttatac ggaacaacat
    22201 ttaactccag cggtctgaaa atgcgcggca acttacgcag caagaacgcc gagctgattg
    22261 aaaaggttag aacatcctat gaaattcaaa acaaacaaac tcagccttaa tcttgtgctt
    22321 gccagctcac ttctggccgc cagcattccg gcatttgccg taaccggaga cactgatcag
    22381 ccgatccaca ttgaatcgga ccagcaatct cttgatatgc aaggcaacgt ggttaccttt
    22441 accggtaatg tcatcgtcac ccagggcacc atcaaaatta atgccgacaa agtggtcgtt
    22501 acccgtccgg gcggcgaaca aggtaaagaa gtgattgacg gatacggtaa accggcaacg
    22561 ttctaccaga tgcaggacaa cggtaaaccc gttgaaggtc acgcttccca gatgcactac
    22621 gaactggcaa aagattttgt cgttctgacg ggtaatgctt atctgcagca ggtcgatagc
    22681 aacattaagg gcgataagat cacttacctg gtgaaagagc agaaaatgca ggctttcagc
    22741 gacaaaggca agcgcgtaac caccgttctg gtgccgtcgc agctgcagga caaaaacaac
    22801 aaaggccaga ccccggcaca gaagaagggt aattaattcg ttatggcaac attaactgca
    22861 aagaaccttg caaaagccta taaaggccgt cgcgtggtag aagacgtcag cctgaccgtc
    22921 aactccgggg aaattgtcgg tctgctgggg ccaaacggtg ccggtaagac caccactttc
    22981 tacatggttg taggcattgt gccgcgcgat gcgggcaaca tcattattga tgatgacgat
    23041 atcagtctgc tgcctctgca tgcacgcgcg cgccgcggta tcggctatct gccacaggaa
    23101 gcctccattt tccgtcgcct cagcgtttac gataacctga tggcggtact gcaaattcgt
    23161 gacgacttgt ctgctgaaca acgtgaagac cgcgcgaacg agctgatgga agagtttcac
    23221 attgagcacc tgcgtgacag catggggcag tcactctccg ggggtgaacg tcgccgtgta
    23281 gaaattgccc gcgcactggc tgcgaatccg aaatttattc tgctcgacga accgtttgcc
    23341 ggggttgacc cgatctcggt tatcgacatt aaacgcatca ttgaacacct gcgcgacagc
    23401 ggcctgggcg tgctgatcac tgaccacaac gtgcgtgaaa cactggcggt ttgtgaacgc
    23461 gcttatatcg tcagtcaggg gcatttgatc gcccacggca cgcctacaga aatcttacaa
    23521 gacgaacacg ttaagcgtgt ataccttggg gaagacttca gactctgata gggtagaagt
    23581 ttgcgacgtt ttagcaggag agtacgattc tgaacatgaa gcaaggtttg caactcaggc
    23641 ttagccaaca actggcgatg acgccacagc tccaacaggc aattcgtctg ttgcagttgt
    23701 cgacgctgga acttcagcag gagctacagc aggcgctgga gagtaatccg ctgcttgagc
    23761 aaatcgacac tcatgaagaa atcgacaccc gcgaaacgca agacagtgaa acgctggaca
    23821 ccgccgacgc gctcgaacaa aaagagatgc cggaagagct gccgctcgat gccagttggg
    23881 acaccattta caccgctggt acaccatccg gcaccagcgg tgactacatt gacgacgagc
    23941 tgccggtcta ccagggcgaa acgacgcaga ccttgcagga ttacctgatg tggcaggtcg
    24001 agctgacacc gttttccgac actgaccgcg ctattgctac ctctatcgtc gatgccgttg
    24061 atgaaaccgg ttatctgact gtcccgctgg aagatattct cgaaagtatg ggcgatgaag
    24121 agatcgacat cgacgaggtt gaagccgtcc ttaagcggat ccaacggttt gatccggtcg
    24181 gtgtagcggc aaaagatctg cgtgactgcc tgctaatcca actctcccaa ttcgataaga
    24241 ccacgccgtg gctggaagag gccagattga tcattagcga tcatctcgat ctgttagcca
    24301 atcacgactt ccgcacttta atgcgcgtca cgcgtctgaa agaagatgtg ctgaaagaag
    24361 ccgtcaatct gatccagtcg ctcgatccgc gccccgggca gtcgatccag actggcgaac
    24421 ctgagtatgt cattccagat gtgctggtgc gtaagcataa cggtcactgg acggtagaac
    24481 tcaacagtga cagcattccg cgtctgcaaa tcaaccagca ctacgcctcg atgtgcaata
    24541 acgcgcgcaa cgatggtgac agccagttta tccgcagcaa tctgcaggat gccaaatggt
    24601 tgatcaagag tctggaaagc cgtaacgata cgctactgcg cgtgagtcgc tgtatcgttg
    24661 aacagcagca agccttcttt gagcaaggtg aagaatatat gaaaccgatg gtactggccg
    24721 atatcgccca ggctgtcgaa atgcatgaat cgacgatatc tcgcgtgacc acgcaaaaat
    24781 acctgcatag tccacgaggc atttttgaac tgaagtattt cttttccagt cacgtcaata
    24841 ccgagggcgg cggcgaagct tcctccacgg cgattcgtgc actggtgaag aaattaatcg
    24901 cggcggaaaa cccagcgaaa ccgttgagcg acagcaagtt aacctctttg ctgtcggaac
    24961 aaggtatcat ggtggcacgc cgcactgttg cgaagtaccg agagtcttta tccattccgc
    25021 cgtcaaacca gcgtaaacag ctcgtttgac ccaaccgata aggaagacac tatgcagctc
    25081 aacattaccg gaaataacgt cgagatcacc gaggcactgc gcgaatttgt tacagctaaa
    25141 tttgccaaac ttgagcaata ttttgaccga atcaaccagg tctatgttgt tctgaaagtg
    25201 gagaaagtca cccacacctc agatgcaaca ctgcatgtaa acggtggtga aattcatgcc
    25261 agcgcggaag gtcaggacat gtacgctgcc attgatggct taattgataa gctggcacgc
    25321 cagttgacca aacataaaga taaactgaaa caacactaat tgtccgggca attagcatgt
    25381 gcatggcggc ctgttgtgcg gcacaacggg ccatttgtac ggttaatgct ccgagcctgt
    25441 tccactgttt gagtgggcag gttcttaggt gaaattatga caaataatga tacaactcta
    25501 cagcttagca gtgttcttaa cagggaatgt acgcgaagcc gcgtccactg tcagagcaaa
    25561 aaacgcgccc tggaaatcat cagcgagctg gcggcgaaac aacttagcct gccgcctcag
    25621 gtggtttttg aagctatcct gacgcgggaa aaaatgggca gtaccggtat cggcaatggt
    25681 attgccattc cgcatggcaa acttgaagaa gatactctgc gtgccgttgg cgttttcgtt
    25741 cagctcgaaa cgcctatagc tttcgatgcc atcgacaacc aaccggtgga cttacttttt
    25801 gccctgctgg tgccggcaga ccaaactaaa acgcacctgc atactctgtc gctggtggcg
    25861 aaacgtctgg cggacaaaac catctgccgc cgtttacgcg cagcccagag cgatgaagag
    25921 ctgtatcaaa tcattacgga taccgaaagt actccggatg aagcgtagtt attcggtaat
    25981 gtctctttta gacgttgtga ggagaaacag tacatggtac tgatgatcgt cagcggacgt
    26041 tcaggttcag gtaaatctgt cgccctgcgt gcgctggaag atatgggttt ttactgcgtg
    26101 gataaccttc ccgtagtgtt gttacccgat ctggctcgaa ctctggccga tcgcgagatt
    26161 tctgccgccg tcagcattga tgttcgtaat atgccggagt caccagaaat attcgaacag
    26221 gcgatgagta acctgcctga cgctttctca ccgcaactac tgttcctgga tgccgaccgt
    26281 aataccttaa ttcgtcgtta cagtgacacg cgccgactgc atccgctttc cagcaaaaac
    26341 ctgtcgctgg aaagtgctat cgacaaagaa agcgatttgc tggagcctct gcgttcgcga
    26401 gcggatctga ttgtcgatac ctcagaaatg tccgttcacg agctggctga aatgctgcgt
    26461 acccgtctgc tgggtaaacg tgaacgcgaa ctgaccatgg tctttgagtc tttcggcttc
    26521 aaacacggta tccctatcga tgcagattac gtctttgacg tgcgcttctt gccgaacccg
    26581 cactgggatc cgaaactgcg tccaatgaca ggtcttgata aacctgtcgc cgcgttcctc
    26641 gaccgccaca cagaagtaca caattttatc taccagacgc gaagctatct tgagctatgg
    26701 ttacctatgc tggaaaccaa caaccgtagc tacctgacgg tcgccattgg ttgtaccggc
    26761 gggaagcacc gttcggtgta tattgcagag caactggcag actacttccg ctcgcgcggt
    26821 aaaaacgtcc agtcacgcca tcgtacgctg gaaaaacgta aaccatgacc gtcaagcaaa
    26881 ctgttgaaat cacaaacaag ctgggcatgc atgcccggcc tgcaatgaag ctgtttgaat
    26941 taatgcaggg ttttgacgct gaagtgctgt tacgtaatga cgaaggcacc gaggctgaag
    27001 ccaacagcgt tattgcgctg ctgatgttgg attctgccaa aggacggcag attgaagttg
    27061 aagcgaccgg tccacaggaa gaggaagcac tggccgccgt tatcgccctc tttaattctg
    27121 gttttgatga agattaatct tcatcacttt gacatacaac tcccttcaag ctcccccgac
    27181 aataagaaaa tcacgtactg aaatcgttct caatcaacgt catttgtaca ttttgtgcgc
    27241 ttttcacttt cagaagaacc ttaagaaaac cttaagaggc attgtttagg ttttgtttaa
    27301 gttaatcgat catactggag atcgtcagaa aatatttcca ggagatggca tgattcgctt
    27361 atctgaacaa agtcccctgg gcaccggaag acatcggaaa tgttatgcgc atccggaaga
    27421 tgcccaacgc tgtatcaaga ttgtctacca tcgtggcgat ggtggcgata aagagatccg
    27481 ccgtgagtta aagtactacg cgcatcttgg tcgccggtta aaagactgga gtggaatacc
    27541 gcgctatcac ggtaccgttg agacggattg cggaaccggt tatgtctacg atgtgattgc
    27601 cgattttgac ggcaagcctt ccattacgct gaccgaattt gctgaacaat gccgttacga
    27661 agaagacatt gcacaactgc gccagctact gaaacagctg aagcgttatt tgcaggataa
    27721 ccgtatcgtg acgatgtcgc tgaagccgca gaatatcctc tgtcaccgca tcagtgagtc
    27781 agaggttatc ccggtcgtct gcgacaacat tggtgaaagc acattgattc cactggcgac
    27841 ctggtcgaag tggtgttgtc tgcgtaagca agagcggtta tggaaacgat ttatcgccca
    27901 accggcgctg gctattgccc tgcagaaaga tctgcaacct cgtgagagca aaacgctggc
    27961 cctcacttcg cgcgaagctt aatccagctg gtgttgttgc ataaacggct cactgcctaa
    28021 ctgatacatc tgccgtaaaa tccacgcctg acggctacgc acgtagcccg atggcgagga
    28081 gactttgaaa cgaagtggat taggtaatac agctgccagt aatgcagctt ccgaccgggt
    28141 aagtttgctc gcgggtttgt ggaaataacg ttgtgccgca gcttcgacgc caaacacacc
    28201 gtcgccaaat tcggcgatat tcaggtaaac cgtcaggata cgctttttgc tccagactgt
    28261 ttctatcccc agcgttaatc cagcttccag cccttttcgt acccagctac gcccatccca
    28321 taaaaagaga tttttggccg tctgctgaga aatcgttgaa gcaccgcgaa tacggttttc
    28381 attgcgctcg ttgtgcgcca gggctttctc aatggaagcg acatcaaagc cccagtgctc
    28441 aggaaatttc tgatcttctg cggcaatcac cgccagtccc atccacggcg agatttgatc
    28501 catactgacc cagtcagaat gtgccacgta acgaaaattg ccatgcagcc aggcgctgac
    28561 ctgtcgctcg accattaccg ctgagaaggg aacaggcgca acgctaaaca acgcgatgcc
    28621 cccgccccag aaaacggcga ggacaaccat taaccgcaaa agaaaacggc gaacgaaact
    28681 aaacaccgtt aagcggcttt tactcattca gccagaacca gcacgcggga aaccagctta
    28741 tcaatgccgc tcgccgcttc tgcaatgttc tgcgccagca tatatgctgg ggtggtgaca
    28801 atcttattgt cttcatcaac cacgatatca tcgacaggac acggcacatg ctccgcgccc
    28861 atctcttcca gcacttctgc ggtatcgata tcagtaccga tggtcaaacg cagcgggaaa
    28921 tcgaaaattt tcggcagcat cgccggggca atacacataa aaccaagcgg ttttccggcc
    28981 tgatgcatcg cttgtgccag ctcctttaat tcacggtcaa cggtgcattc gctaccaaga
    29041 ctggcaaaat tgcttaaatt cttcgccgcg ccaaaccccc ccggcacaat caacgcatcc
    29101 agttcagcgg catcggcctg ggccagagga cggatttcac cgcgcgttat tcgtgccgct
    29161 tcaatcagca cattgcgcgt ttccgtcatc gcttcgccag ttaaatggtt gataacatca
    29221 acctgctgct tatccggtgc aaagcagacc gcctgcgcac cgctgcgtga aatagctaac
    29281 aacgtcaaca ccgcttcatg aatttcagaa ccgtcataga cgccgcatcc gctcagaatt
    29341 acgccaattt tcttcattgt gatcatcctt tcgcaattga ctgaaacaca ttaatttttt
    29401 taataaaaat ggtacgcatc acacatttaa ctgattcatg taacaaatca tttaagtttt
    29461 gctatcttaa ctgcgtgcgg cctgaaaaac agtgctgtgc ccttgtaact catcataata
    29521 atttacggcg cagccaagat ttccctggtg ttggcgcagt attcgcgcac cccggtctag
    29581 ccggggtcat tttttagtgg cttttgccac ccacgctttc agcacttcta cgtcgtgacg
    29641 ccactcttct ttcatctctt caatccattc accgacgtta tcttcccagg cgggaaggtc
    29701 aggagactga atttgctgac ccagctgttg cagatggcgt aaccccactg accccgccgc
    29761 acctttaatt ttatgtcctt cctcaacaat gcctttttta tcctgcgccg tcagattcga
    29821 ctccagcacg ctgacatagc ccggcatcat cttctcaaac accgctaacc cgtcggtgat
    29881 cagcttcggt cctacaagtt cgagatactg ttccagcatg ggaatatcga gcaatgcttc
    29941 tgatttactg ttctcttctg tcgtcaccgt actctcctca tcatcctggg tatcccagaa
    30001 tttcttgatc atcgcggtta gcgccggaac cgaaagcggc ttactcagca catcatccat
    30061 tccagcattg aggtactctt gtttgtcttt cagcacgtta gcggttaagg ccaccagcgg
    30121 cggtaaatcc tcgcgcggat aacgcttcgt cagttcacga gagatatcca gcccggtcat
    30181 atctggcaac tgaatatcca gcaacaccag gtcgtattcg cccggtttaa acatctccag
    30241 cgccgccttg ccggtcatgg cgacatcaac gctgttacct aatttttcca gcacagaacg
    30301 cgcgacaatc acgttcagtt caatgtcttc caccagcagc acattcagcg ccggtaaagg
    30361 catatcgtct tcatcaaacg catcatcgac ctcttctgcc accgacggtg cgtggatcgt
    30421 caacgtaaag gttgaacctt tgccctgttc gctggtaacc gtaatatcgc cacccatatt
    30481 tttcgccaga cgacgagaaa cggccagacc aataccggtg ccggtggcag gtttaccgcc
    30541 atgactgtct ttcacctggt aatacatggc gaagatttta tccagctcat cctgcggaat
    30601 gccgatacca gaatcttcca cttcaaaatg cagcatatcg ccttcatcgt agcgcacgcg
    30661 cacggtaacc tggccttgct gggtgaattt gacggcgtta ctgatgaggt tccacaggat
    30721 ctgccgtaaa cgcgtcccgt cggtaatgac ctgatgcggt aatggcagcg taggctccag
    30781 gttaaagcgc aatccttttt gttgcgcctg caatgcggag agattttcca gatcggcaag
    30841 gaagctggtg aaatcaaccg gctgattatc aagctggacc ttgcgccgtt ccatcttatc
    30901 catgtcgata atatcgttaa agatattccc cagcgtgacg gccgaaacat ggatagtttt
    30961 gagatatttt tcctgctcgg cggtgagttc ggtatccagc agaatgcggc tcaggccgac
    31021 gataccgttc agcggtgtac gcaattcgtg actgatggtg gaaataaacg tcgttttgtc
    31081 acggctggcg cgttcaagcg catcctgata ccgcttacgc tcggtaatgt cgcgaccaaa
    31141 gcccatcaaa ccgtgacgtt tacccacgcg gtcgtagtac ggcactttac ggatttcaaa
    31201 gcaggctttg cgcccgtccg ggtaatccag ccactgttca taggtcagtg acacattatg
    31261 acggaacact ttttcatcgg tttcaatgac ttttgcggcg gcttccggtg agtaaacatc
    31321 agcaggtttc aggtgaacca gttgtttttc gctttttccg gtcagcagct ccatcgcgcg
    31381 gttacagccg gaaaactctt tatcttcgtt acgataaaaa accaggtcgg gtgaagcatc
    31441 aaggaaggaa cgtaagaatg aggattgctg ctcgagctga atttgtgtct cttcgcgctc
    31501 tttgatttca attttcagtt ggccgaaggt ttcctgcagt tctgcttccg ctttttcacg
    31561 aacggcaatt tcctgattta gctgggcaat attatctttt aactgaacgt tgaggctcaa
    31621 atcgcgctcg cgcatctcct ccagtttttg caccagccgt gacagacgtt gtcgtgactc
    31681 ctccagttgc tcgacgacca ccgatagaaa gtagaccgcc cacggcgtaa tcagcaaacc
    31741 aaagaagata gaacgaataa catcaatgct ttcgacctga ccatgcagca ccatggttac
    31801 cgccatttgt accacaatgg caagaacgac gagggccagc gccagcaaca ttgagaagcg
    31861 caccagacct aacttcatca tcaggtcaac ataatactgc gccagcagac gaatttgctt
    31921 cattagggaa ttccttcacg acaacctgcc ccaataatac ccaattctga cagttacgtt
    31981 ataaattgtg tgagaaatgc ggaattcccc tcaacgacct actccgttgg aggtagccac
    32041 ggacgtccca gcgccgatcc ctgtacgcca tgttcattca gatagcgatc cagctcgacc
    32101 attcccgtcc atcgattttc gcaccacagc ggagcaagca gcgtcgggcg acgggcgctg
    32161 gcggaaatgc ggtggtagat cacttccggt ggcgtatggc gaatcatttc tccggcagtg
    32221 agcgtataat cctccagttc aataccgttc aaacgccccg cttcccaggc tttcgccata
    32281 atgctgcctt tcacaatatg cagcggatgc agctttatgc cgtccacgcc ggtttcaacc
    32341 actcgctcca gcgtttgcaa acattcagcc tgcccttcgc caggcaggcc gacaattaag
    32401 tgggaacaca ccttcagccc gcgctgacgt gccagctggg ttgtacgctg ataacaggca
    32461 aaatcatggc cgcggttgat gcgatgcagt gttttgtcgt gggcggtttg tagccccagc
    32521 tccagccaca cttcgtaacc ctggtcctta tattcgcaaa gcagatccag caccgcatcc
    32581 ggcacgcagt ccgggcggat accaacacac aaaccgacaa tattggcctg gctcaccgcc
    32641 tgctgataca tagaacgcag cacctgaact tccgcaaagg tgctggtata cgcctgaaag
    32701 taggccagat agcgtttagc gcggttaact aaattcgcct ggtgcgccag ttgctcggca
    32761 atggaacgat gctgctgcgc ttcatcggca aacgaggcaa cattacagaa tgtgcagcca
    32821 ccacgcccga tggtaccgtc acggttaggg cagctaaaac cgccgtgcag cgtcagctta
    32881 tgcacctttt gcccataacg acgggtgaga tcaccaccaa acatattgac taatttctgt
    32941 aactgcataa tctgatagac cgcgccttga aaagaggcca aagcctgcca tttttagctc
    33001 aattcggcga tgacctggat caatcgtccc ggcctgcttt tatcaactgc ataatcaatc
    33061 aaaattaccg aaatttcatg cataatcaca taaatcactt ttgcttatct tgtgtcagat
    33121 ttttttatct ccttatggat tttaggcaaa aacagtagca tgaaacgtca ttaccaatta
    33181 aggcagtata aaatgctggt tttctcgtca gttcaaggca ggataagggt taacacgcct
    33241 ttatgacagt caggaattga ctgtttctct aacgactctc cttttagcct taaagataaa
    33301 atctatttta atttcagcta tttaataaag aattttgcgc taaagcacat ttctgtacca
    33361 ataagcttgc catttgacct gtatcagctt tcccgataag ttggaaatcc gctggaagct
    33421 ttctggatga gcagcctgct catcatattt atgcagtaat tgagatcccc tcttcaccgt
    33481 attaaccgat gcgaaaagga caacaagggg gcgaatgcga ggcgcgcgta tgacacgcaa
    33541 accccgtcgc cacgctcttt ctgtgcccgt gcgcagcggt tcggaagtgg ggttcccgca
    33601 gagcctaggg gaggttcacg atatgttgta cgataaatcc cttgagaggg ataactgtgg
    33661 tttcggcctg atcgcccaca tagaaggcga acctagccac aaggtagtgc gtactgcaat
    33721 acacgcactg gcccgcatgc agcaccgtgg cgcgattctc gccgatggta aaaccggcga
    33781 cggttgcggc ttgctgttac aaaaaccgga tcgctttttt cgcatcgttg cgcaggagcg
    33841 cggctggcgt ttagcaaaaa actacgctgt cgggatgctc ttcctgaata aagatcctga
    33901 actcgccgct gccgcacgcc gcatcgttga agaagaactg caacgcgaaa ccttgtcgat
    33961 tgtgggctgg cgtgatgtcc ccactaacga aggcgtgctg ggtgaaatcg ccctctcctc
    34021 tctgccacgc attgagcaaa tttttgtgaa cgccccggca ggctggcgtc cacgcgatat
    34081 ggagcgccgt ctgtttatcg cccgccgccg cattgaaaag cgtctcgaag ccgacaaaga
    34141 cttctacgtc tgtagcctgt cgaatctggt gaacatctat aaaggtctgt gtatgccggc
    34201 ggatctgccg cgcttttatc tggatcttgc ggacctgcgt ctggaatcgg ccatttgcct
    34261 gttccaccag cgcttctcca ctaacaccgt accgcgctgg ccgctggcgc aaccgttccg
    34321 ctatctggcg cataacggtg aaatcaacac catcaccggt aaccgccaat gggcgcgtgc
    34381 gcgtacctat aaattccaga cgccgctgat ccctgacctg cacgacgccg cgccattcgt
    34441 caacgaaacc ggttctgact ccagttccat ggataacatg ctggaactgc tgctggcagg
    34501 cgggatggat atcatccgcg ccatgcgcct gttagtgcca cccgcctggc agaacaaccc
    34561 ggatatggac ccggaactgc gtgccttctt tgactttaac tccatgcata tggagccgtg
    34621 ggatggtccg gcgggcatcg tgatgtccga tggtcgcttc gctgcctgta acctcgaccg
    34681 taacggtctg cgtccggcac gctacgtcat taccaaagac aagctcatca cctgcgcctc
    34741 tgaagtcggt atctgggatt accagcctga cgaagtggtc gaaaaaggcc gcgtcggacc
    34801 aggcgaactg atggtgatcg acactcgcag tgggcgtatt ctgcactcgg cagaaaccga
    34861 tgacgatctg aaaagccgcc acccttataa agagtggatg gagaaaaacg tccgccgact
    34921 ggtaccgttt gaagatctgc ccgatgaaga agtgggtagc cgcgaactgg acgacgacac
    34981 gcttgccagc taccagaaac agtttaacta cagcgcggaa gagctggact ccgtaattcg
    35041 cgtactgggc gaaaacggtc aggaagcggt cggttcgatg ggcgatgata ccccattcgc
    35101 cgtgctctcc agtcagccgc gcattattta cgactacttc cgccagcagt ttgcccaggt
    35161 gactaacccg ccaatcgacc cgctgcgtga agcgcatgtt atgtcgctcg ccaccagtat
    35221 cggtcgtgaa atgaacgtct tttgcgaagc agagggccag gcgcaccgtt taagctttaa
    35281 atcgccgatt ctgctctact ccgatttcaa acagctcacg acgatgaaag aggagcacta
    35341 ccgcgcagat acgctggata tgacctttga cgtcactaaa accacgctcg aagcgacagt
    35401 caaagagctg tgcgacaaag ccgaaaaaat ggtacgtagc ggcaccgtgt tgctggtgct
    35461 ctccgaccgg aatatcgcta aagatcgcct gccggttccg gctccgatgg cggttggcgc
    35521 gatccagacc cgtctggtcg atcaaagcct gcgttgcgat gccaacatca tcgtcgaaac
    35581 cgccagcgcc cgcgatccgc accacttcgc cgtgttgctg ggtttcggcg cgacggctat
    35641 ttatccgtac cttgcctatg aaacgctggg ccgcctggta gacacccacg cgattgccaa
    35701 agattatcgt accgtgatgc tcaactaccg taacggcatc aacaaaggct tgtacaaaat
    35761 catgtccaaa atgggcatct ccaccatcgc ctcttaccgc tgctcgaaac tgtttgaagc
    35821 ggtcggtcta cacgatgatg tagtgggcct gtgcttccag ggcgcggtca gccgcattgg
    35881 tggggcaagc tttgaagact tccagcagga tctgctgaat ctgtcgaaac gtgcctggct
    35941 ggcgcgcaag ccgattagcc agggcggcct gctgaaatac gtccacggcg gcgaatacca
    36001 cgcctacaac ccggacgtgg tgcgcacgct gcaacaggcg gtacaaagcg gcgagtacag
    36061 cgactatcag gaatacgcga agctggttaa tgagcgtccg gcaaccacgc tgcgcgatct
    36121 gctggcaatt acgccgggtg aaaacgcggt caacattgct gatgttgaac cggcaagcga
    36181 actgtttaaa cgctttgata ccgccgcgat gtctatcggc gcgttaagcc cggaagccca
    36241 cgaggcgctg gcggaagcga tgaatagcat cggcggtaac tcgaactccg gtgaaggcgg
    36301 cgaagacccg gcgcgctatg gcactaacaa agtgtcgcgc atcaagcagg tggcttctgg
    36361 tcgcttcggc gttactccgg cgtatctggt caatgccgac gtcattcaga ttaaagtcgc
    36421 ccagggcgcg aagccgggcg aaggcggtca gttgccgggc gacaaagtca ctccttacat
    36481 cgccaaactg cgctattcgg tgcccggagt gacgctgatc tccccgccgc cgcaccacga
    36541 tatctactct atcgaagact tagcgcagct tattttcgac ctcaagcagg ttaacccgaa
    36601 agcgatgatc tccgttaagc tggtgtccga accaggcgtt ggcaccatcg cgactggcgt
    36661 ggcgaaagct tatgcggact tgatcaccat cgcgggctat gacggcggca ccggcgcaag
    36721 cccgctgtcg tcagtgaaat atgcaggctg cccgtgggaa ctcggtctgg tggaaaccca
    36781 acaggcgctg gttgccaacg gcttgcgtca taaaattcgt ttgcaggttg atggcggcct
    36841 gaaaacgggt gtcgatatca tcaaagcggc gattctcggc gcagaaagct tcggcttcgg
    36901 cactggccca atggtggcgc tcggctgtaa atatctgcgt atttgccacc tgaacaactg
    36961 cgccacgggt gtagcaactc aggatgacaa actgcgtaag aatcactatc acggcctgcc
    37021 gttcaaggtg actaattact ttgagtttat cgcccgtgaa acccgcgagc tgatggcaca
    37081 gctgggagta acgcgtctgg tggatctgat tggtcgcacc gacctgctga aagagctgga
    37141 cggtttcacc gccaaacagc agaaactggc gctgtcgaag ctgctggaga ctgccgagcc
    37201 acatgcaggt aagtcactct actgcactga aaacaacccg ccgtttgata acggcctact
    37261 gaacgcgcag ttgctgcaac aggcgaaacc gtttgtcgat gaacgccaga gcaaaacctt
    37321 ctggttcgat atccgcaaca ccgaccgttc tgtcggcgcg tcgctttcag gctatatcgc
    37381 ccagacgcac ggcgatcagg ggctggcagc cgatcctatc aaagcgtact tcaacggcac
    37441 cgcaggccag agtttcggtg tgtggaacgc gggcggcgtg gaactgtacc tgaccgggga
    37501 tgccaacgac tatgtcggta aaggcatggc gggcggctta atcgccattc gtcctccggt
    37561 tggttccgcc ttccgcagcc atgaagcaag cattatcggc aacacctgcc tgtatggcgc
    37621 gaccggtggt cgtctgtatg ccgcaggccg cgcgggtgaa cgtttcggcg tgcggaactc
    37681 cggtgctatc accgtggtag aaggcattgg cgacaacggc tgtgaatata tgacgggcgg
    37741 tattgtctgt attctgggta aaaccggcgt taacttcggc gcgggcatga ccggcggctt
    37801 cgcttacgtt ctcgatgaaa gcggtgattt ccgcaaacgc gttaacccgg aactagtcga
    37861 agtcttaagc gtcgacgatc tggcgatcca tgaagagcat ctgcgcggtc ttatcaccga
    37921 gcatgttcag cataccggtt ctcagcgcgg tgaagagatt ctggcgaact ggtcaacctt
    37981 cgccacgaaa ttcgcgctgg ttaaaccgaa gtccagtgat gtaaaagcac tgctgggtca
    38041 ccgtagtcgt agcgcagcag agttgcgcgt gcaggcgcag taaggggtag caacaatgag
    38101 tcagaatgtt tatcaattta tcgacctgca gcgcgttgat ccgccaaaga aaccgctgaa
    38161 gatccgtaaa attgagtttg ttgaaattta cgagccgttt tccgaaggcc aggccaaagc
    38221 gcaggctgac cgctgtctgt cgtgcggcaa cccatactgc gagtggaaat gcccggtaca
    38281 caactacatc ccaaactggc tgaagctcgc caacgagggg cgtatttttg aagcggcaga
    38341 actgtcgcat cagaccaaca cactgccgga agtatgcgga cgtgtctgcc cgcaagaccg
    38401 tctgtgcgaa ggttcctgca ccctgaacga tgagttcggt gcggtgacca tcggcaacat
    38461 tgagcgttat atcaatgata aagcgttcga aatgggctgg cgtccggata tgtccggcgt
    38521 gaagcaaacc ggtaaaaaag tggcgattat cggtgcaggc ccggcaggtc tggcgtgtgc
    38581 ggatgtcctg acgcgtaacg gcgtaaaagc cgttgtcttc gaccgtcatc cagaaattgg
    38641 cgggctgctg accttcggta ttccggcctt caagctggaa aaagaggtaa tgacgcgtcg
    38701 ccgtgaaatc ttcaccggca tgggtattga attcaaactc aataccgaag tgggccgcga
    38761 cgtacagctg gacgatctgc tgagtgatta cgatgccgtg ttccttggcg ttgggactta
    38821 tcagtcaatg cgcggcgggc tggaaaacga agacgccgat ggcgtgtacg cagcgctgcc
    38881 gttccttatc gccaacacca aacagttaat gggctttggt gaaacccgcg acgaaccgtt
    38941 cgtcagcatg gaaggcaaac gcgtggtagt acttggcggt ggcgacactg cgatggactg
    39001 cgtgcgcacg tctgtacgcc agggggcaaa gcacgttacc tgtgcctatc gtcgtgatga
    39061 agagaacatg ccgggttccc gccgcgaagt gaaaaacgcg cgggaagaag gcgtagagtt
    39121 caaattcaac gtccagccac tggggattga agtgaacggt aacggcaaag tcagcggcgt
    39181 aaaaatggta cgtaccgaaa tgggcgaacc ggatgccaaa ggccgtcgcc gcgcggagat
    39241 cgttgcaggt tccgaacata tcgttccggc agatgcggtg atcatggcgt ttggtttccg
    39301 tccacacaac atggaatggc tggcaaaaca cagcgtcgag ctggattcac aaggccgcat
    39361 catcgctccg gaaggcaacg acaacgcttt ccagaccagc aacccgaaaa tctttgctgg
    39421 cggcgatatc gtccgtggtt ccgatctggt cgttaccgcc atcgccgaag gtcgcaaagc
    39481 ggcagacggc attatgaact ggctggaagt ttaagctctg tcccagcgcc gcctcctcag
    39541 aaaagagtcg gcgctgaaat tctcctttct ttctggaaat cgcctcgcat aatctgaaga
    39601 aaaccaatgt aatacgctca tctggattgt taccggtaga tgcccggcgt atagctgatt
    39661 ccattattca acgcagcgcc atataaaggg acagataatg gaatcgctct cagagagaac
    39721 ctcaacaggc taccagcaaa tccacgacgg cattattcat ctggtcgata gcgcccgaac
    39781 ggaaacggta cgtagcgtta acgcgctgat gaccgcgacg tactgggaaa ttggccgacg
    39841 gattgtcgaa tttgaacaag gtggtgaggc cagagcagct tatggtgcac agttgattga
    39901 tcggctatcg aaggatttaa gtcgacggta taaacgtggg gtctctgcaa aaaacttacg
    39961 ccaaatgagg cttttttacc tcttttttca acatgttgaa attcgccaga cagtgtctgg
    40021 cgaattaaca ccattgggaa ttccgcagac accatctgca gaattcccat cggcaaagat
    40081 ttggcagacg ctgtctgcca aattgagagg ctgtttgata tttccacagc ttttgtattg
    40141 catctcctat tttcggtttg tatattcatg caaataaacg aagg
//
LOCUS       DADLSA010000021        39385 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.021, whole genome shotgun sequence.
ACCESSION   DADLSA010000021 DADLSA010000000
VERSION     DADLSA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.021"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(71..1324)
                     /locus_tag="JN691_001125"
     CDS             complement(71..1324)
                     /locus_tag="JN691_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAY7242622.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1557..2888
                     /gene="argA"
                     /locus_tag="JN691_001126"
     CDS             1557..2888
                     /gene="argA"
                     /locus_tag="JN691_001126"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAY7242623.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2950..4776)
                     /gene="recD"
                     /locus_tag="JN691_001127"
     CDS             complement(2950..4776)
                     /gene="recD"
                     /locus_tag="JN691_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HAY7242624.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMFEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4776..8318)
                     /gene="recB"
                     /locus_tag="JN691_001128"
     CDS             complement(4776..8318)
                     /gene="recB"
                     /locus_tag="JN691_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAY7242625.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="JN691_001129"
     CDS             complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="JN691_001129"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAY7242626.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKDGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AIITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11375..14743)
                     /gene="recC"
                     /locus_tag="JN691_001130"
     CDS             complement(11375..14743)
                     /gene="recC"
                     /locus_tag="JN691_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAY7242627.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKTLVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14756..15079)
                     /locus_tag="JN691_001131"
     CDS             complement(14756..15079)
                     /locus_tag="JN691_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY7242628.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15064..15471)
                     /locus_tag="JN691_001132"
     CDS             complement(15064..15471)
                     /locus_tag="JN691_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAY7242629.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHCWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15468..16031)
                     /locus_tag="JN691_001133"
     CDS             complement(15468..16031)
                     /locus_tag="JN691_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY7242630.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16022..16492)
                     /locus_tag="JN691_001134"
     CDS             complement(16022..16492)
                     /locus_tag="JN691_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY7242631.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLYQQGETEGCL"
     gene            complement(16676..17470)
                     /gene="thyA"
                     /locus_tag="JN691_001135"
     CDS             complement(16676..17470)
                     /gene="thyA"
                     /locus_tag="JN691_001135"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAY7242632.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17477..18352)
                     /gene="lgt"
                     /locus_tag="JN691_001136"
     CDS             complement(17477..18352)
                     /gene="lgt"
                     /locus_tag="JN691_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAY7242633.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18503..20749)
                     /gene="ptsP"
                     /locus_tag="JN691_001137"
     CDS             complement(18503..20749)
                     /gene="ptsP"
                     /locus_tag="JN691_001137"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7242634.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20762..21292)
                     /gene="rppH"
                     /locus_tag="JN691_001138"
     CDS             complement(20762..21292)
                     /gene="rppH"
                     /locus_tag="JN691_001138"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAY7242635.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            21977..22666
                     /gene="mutH"
                     /locus_tag="JN691_001139"
     CDS             21977..22666
                     /gene="mutH"
                     /locus_tag="JN691_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAY7242636.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22735..23448
                     /locus_tag="JN691_001140"
     CDS             22735..23448
                     /locus_tag="JN691_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY7242637.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23586..23804
                     /gene="ygdR"
                     /locus_tag="JN691_001141"
     CDS             23586..23804
                     /gene="ygdR"
                     /locus_tag="JN691_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAY7242638.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23912..24952
                     /locus_tag="JN691_001142"
     CDS             23912..24952
                     /locus_tag="JN691_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAY7242639.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(24985..26178)
                     /gene="lplT"
                     /locus_tag="JN691_001143"
     CDS             complement(24985..26178)
                     /gene="lplT"
                     /locus_tag="JN691_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAY7242640.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26171..28330)
                     /gene="aas"
                     /locus_tag="JN691_001144"
     CDS             complement(26171..28330)
                     /gene="aas"
                     /locus_tag="JN691_001144"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HAY7242641.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSCQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28915..29946
                     /gene="galR"
                     /locus_tag="JN691_001145"
     CDS             28915..29946
                     /gene="galR"
                     /locus_tag="JN691_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAY7242642.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29953..31215)
                     /gene="lysA"
                     /locus_tag="JN691_001146"
     CDS             complement(29953..31215)
                     /gene="lysA"
                     /locus_tag="JN691_001146"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAY7242643.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31337..32272
                     /gene="lysR"
                     /locus_tag="JN691_001147"
     CDS             31337..32272
                     /gene="lysR"
                     /locus_tag="JN691_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAY7242644.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32259..32951)
                     /gene="ygeA"
                     /locus_tag="JN691_001148"
     CDS             complement(32259..32951)
                     /gene="ygeA"
                     /locus_tag="JN691_001148"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY7242645.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33080..34498)
                     /locus_tag="JN691_001149"
     CDS             complement(33080..34498)
                     /locus_tag="JN691_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY7242646.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(34790..35551)
                     /gene="kduD"
                     /locus_tag="JN691_001150"
     CDS             complement(34790..35551)
                     /gene="kduD"
                     /locus_tag="JN691_001150"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAY7242647.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(35581..36417)
                     /gene="kduI"
                     /locus_tag="JN691_001151"
     CDS             complement(35581..36417)
                     /gene="kduI"
                     /locus_tag="JN691_001151"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAY7242648.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(36704..37878)
                     /locus_tag="JN691_001152"
                     /pseudo
     CDS             complement(36704..37878)
                     /locus_tag="JN691_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
     gene            38133..39362
                     /locus_tag="JN691_001153"
     CDS             38133..39362
                     /locus_tag="JN691_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY7242649.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLCIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPSSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNHA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         9705 a        10704 c         9627 g         9349 t
ORIGIN      
        1 attgaacacc ctaacgggtg tttttttgtt tctgatctcc cataaaaaag cgccattcag
       61 cgccttttta tcatcccctt ctcgccagcg ttgccccatc ggcaaaatac gctttaatcc
      121 ccgcaagaat agactccgca acttcctgct ggaaagtcgc cgttttcagt ttacgctctt
      181 cctcaacgtt actgataaac gccgtttcga ccagaatgga gggaatatct ggtgccttta
      241 gtacggcaaa cccggcctgt tcaacttgat ttttatgcag cttgttgatt tttccgagct
      301 tattcagtac cgctttacca aacttaaggc tgtcagcaat agtcagcgac tgcaccatat
      361 cgaacatggt gtggtcgaca tagcggtcac cgcttttgct cacgccacca atcaagtccg
      421 aggcgttctg ggtttgtgcc agatattttg ccgcagtact ggttgcgcct ttggttgaga
      481 gcgcaaacac cgaggaaccg ctcggctgac gactggtaaa ggcgtcggca tggatagaga
      541 caaacaggtc agcacgctgt ttctgggctt ttgctacgcg cacttgcaac ggaatgaaga
      601 tgtcttcatt gcgcgtcatg tacaccttca tattgccctc tttctcgatc agagagcgca
      661 aacggcgagc tatttgcaat accacatctt tttcgcgcgt tttgtatttc cccaccgcac
      721 cggagtcttc gccaccgtgg ccagggtcaa gcataatgac aatcggacga tcgcgccctg
      781 ctttacccgg ttgtggacca ctttgcgctg gcggcacctg cttttcgagg tcgcctttgt
      841 tgtaatcctc cagcagcgcc agcagcgggt cctgcatatc ctgtgcattg gccggataga
      901 ggtccatcac cagacgctct ttaaacccgg cgaccggcgc aagggcaaac agctgcggtt
      961 ttacgttttg ctttaattca aaaaccatgc gtacggtttg cgggtcaaat tgcccgacgc
     1021 gcgccgactt gatgaacggg tcgtctgcac gaatttgtgc cgccatcccc ttgagtaccg
     1081 agttcaggtt tacatcttcg atatccacca ccacgcgttc agggttactc aacgcgaact
     1141 gcttatattt cagctgacga tttgattcta ccgtcacgcg ggtgtagctg gacgcaggcc
     1201 agacgcgcac cgccacgacc tggctaaccg cagccaggct gacctgactt acgctcaata
     1261 gccacatagc gcccgcgcct tgcagtaaac gacgacggct gattgcagtg ttggatcctg
     1321 acatgcctct cccgagcaaa agaaatctaa gctgtgtaac aagtaaacga ctaatttgac
     1381 cggaaacttt agcgaaagac gcataatctg tcatctaata aacggtaaac attcttttta
     1441 tattcacggc attactgata aaaaagtcgc tctcgcataa aatttacact tgcaccctgc
     1501 gaaaaaacag aataaaaata caataatttc gaataatcat gcaaaggggg tgtgccgtgg
     1561 taaaggaacg taaaaccgag ctggtcgagg gattccgcca ttcggttccc tatatcaata
     1621 cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt
     1681 tctccagtat cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg
     1741 tctatggcgc acgtccgcag atcgacgcaa atctggctgc gcatcaccac gaaccgctgt
     1801 atcacaagaa tatacgtgtg accgacgcca aaacactgga actggtgaag caggctgcgg
     1861 gaacattgca actggatatt actgctcgcc tgtcgatgag tctcaataac acgccgctgc
     1921 agggcgcgca tatcaacgtc gttagtggca attttattat tgcccagccg ctgggcgtcg
     1981 atgacggcgt ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgatcc
     2041 atcgtcaact ggaaagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg
     2101 gcgagagctt taatctgacc tcggaagaga ttgccactca actagccatc aaactgaaag
     2161 ctgaaaagat gattggtttt tgctcttccc agggcgttac taatgacgac ggtgatattg
     2221 tctccgaact tttccctaac gaagcgcaag cacgggtaga agcccaggaa gagaaaggcg
     2281 attacaactc cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg
     2341 tgcgccgctg tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct
     2401 cacgcgacgg tatcggtacg cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa
     2461 caatcaacga tattggcggt attctggagt tgattcgccc gctggagcag caaggtattc
     2521 tggtgcgccg ttctcgcgag cagctggaga tggaaatcga caaattcacc attattcagc
     2581 gcgataacac gactattgcc tgcgccgcgc tctatccgtt cccggaagag aagattgggg
     2641 aaatggcctg tgtggcagtt cacccggatt accgcagttc atcacggggc gaggttctgc
     2701 tggaacgcat tgccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca
     2761 cgcgcagtat tcactggttc caggaacgtg gatttacccc ggtggatatt gatttactgc
     2821 ccgagagcaa aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt
     2881 tagggtaagc gatgaaaatc accggatgcg gcaggcgtaa cactcgtgcg tcgcatccgg
     2941 caattacgtt tattcccgcg aactaaacaa cgccgccaga ccactgcgcc gctcagtacg
     3001 agtggcgatt gccgcactta atatacgctc atcggcatac agcgacagac gacgacgcgc
     3061 gcgggtcacc gcggtgtata ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa
     3121 aatcaacgcc gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt
     3181 agtttcgtgc tctggcaagc gactcggttg cacagactta atattgccgt ccggcatcgc
     3241 aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa
     3301 cccaagcgcg ctgtcattac gggcaatcat caccggtcgg ccttcgtacc aacgagagtg
     3361 cggatgacga tgaattttgc gcttctgttg cataaactgc tcaattcgct cattcagtcc
     3421 agccacgcca aacggccctt cccgcagggc gcacaaaagc tggtactcat tgaacgcctg
     3481 aatgattaaa tccggctcgg cacgcgcttg cagcagatcc agataacgtc cgtaacccgc
     3541 aagagcttcc tcaaacatcg caatataatc ttcgccgctc tgtaaaagtc gtttttcgat
     3601 atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc
     3661 cgcagctaac tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca
     3721 gagactgtcg cgcaaagatg ccgcttctgt gccggttcct gccggaacgt gagtccccgt
     3781 caggcggctt agctgcctgg cacgctcggc ggtaaagccc gcgttggcat aagcgcagat
     3841 atcgcccagc acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat
     3901 cactcgcgca tgatctggca aggcgtcgat cagtctcgac atcataggca gatcgatcat
     3961 tgacgcttca tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa
     4021 acgctggcta cccggctgcg cgcccagcaa tcggtgcaaa gtgctggcat cttccggaat
     4081 gcgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt
     4141 taagcgcgcg gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc
     4201 ggccatttga attaacgctg ccagcaactt cgccacggtg gtcgttttac cggtgcctgg
     4261 gccgccggaa atcaccgaga tccgccgcgt cagcgccact gccgctgcaa ctttttgcca
     4321 gttaatttca tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc
     4381 aacctcaatg gcatgattca cctcattgaa gaagcgcgct accgtccgct cgttacacca
     4441 catgcgattc aaataaagac gatcgccaca gaggatcatc ggcgtgggtt catctcccct
     4501 gctgaccgct tgagaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact
     4561 gacacaggtc gccaacagcg gatgcgacgc ctcgttattt tccagtcgtg aaagcggcaa
     4621 acagacgtgt ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacggcagg
     4681 atgttcatct cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg
     4741 ctccacagct tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg
     4801 gcaaacatct catccatcag ggcaatcaac ccggcgttgg gtcgggttgt gtaaatccct
     4861 tgttgcggat gttctttatc aacgccacgc aggaacagat agataacgcc gccaaagtga
     4921 agatcatagt cgtaatcagc aatgcgatgg cgcagataac gatgcagcgc cagggtataa
     4981 agctgatatt gcagatcata gcggtgtgcc tgcatcgctg ctgccatagc ctgttgggtg
     5041 taagccgaac tgtcttcacc cagccagttg gatttatagt cgagcaggta ataacgccct
     5101 tcgtggcgga ataccaggtc gataaagcct tttaacatgc cgcgtacctg catgaactcc
     5161 agcggcgggc agccagcgga tagcgggtca aactggcgaa ttagtgcatc aagctggctg
     5221 gcgatgagcg gttcgctaat cggcaggtaa aactccatct ccacctgttt atcgcggtcg
     5281 gaaagctgac tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc
     5341 cactcggtca acaccggttc ccactgcgat tcaaagccgc cgagttccag tttttcctgt
     5401 acccagttcg ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat
     5461 gtccccggtg acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg
     5521 ctgaccacgc ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga
     5581 ccacgctgct gcaagccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt
     5641 agtgtcctcg cattcagttc tgcagttaaa gcatcattaa cctgccaggg ctggttatca
     5701 ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga
     5761 agccctgccg catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg
     5821 acgtcggtgt cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg
     5881 caatgccaaa ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt
     5941 tcgacctccg cgaggtcgac gctttctggc gcagcattaa gatccaaaac tgcctcaaac
     6001 gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga aattggtaat aaatggcaac
     6061 cagaccagcg gatattccag ccctttcgat ttatggatcg tgacaatctg caccagatgt
     6121 ttatcacttt cgaggcgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt
     6181 tgcgataacc agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgaagc
     6241 agttcgctga tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag
     6301 ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg
     6361 cgccagattt gccgataacc atcgaactct tcgactaccg catcccacgc atgttcgtca
     6421 ttattcagcg tttcaatatc cagcgcattc agccccatca ttgacgttgc cagcgcactg
     6481 cgcagggtgt tctcacgttc gggcgtcatc accgcctgca acaaccaaag catttcctgc
     6541 gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc
     6601 aacgttaagg catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacgctgatg
     6661 tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt
     6721 ccggcttgta gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgatag
     6781 tcgccaacgc cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca
     6841 cctttaaata caaaacgtaa cgcctgattt ttcccggctg atttcactgg aataaacggt
     6901 atttcgcgaa acatgaacgc gtcatcagtc tggctgaaaa gcttgttcac actgttcacc
     6961 attccaggtg cggagcgcca gttggtgtct aaagtgtagt gggcgtgaac ttcactacgc
     7021 gccttcatat aagtgaagat atccgcaccc cggaatgcat atatggcctg cttcgggtcg
     7081 ccaatcagca acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac
     7141 tgctgggggt cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc
     7201 gccgctgcca acacctcgcc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg
     7261 tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca
     7321 gccaatgcgc gggtgatcac cagatcgcgg atcgacaatg gttctgcaag cagttgatcg
     7381 atcgcctcaa acagtggatg tcgcggggtt tcccccccgg ccttcgtgcg atcttctaag
     7441 aaacgctggg agaatttttc cagcgactcc ggcaactgat aactgtttgt ctcttcttct
     7501 gcccaggcgc tgatcttttc gatccattta gcctgattgc tacggttaaa cttgcgtcga
     7561 tcaataccag aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt
     7621 tttaccgtat caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg
     7681 ggcggcggtg ctttgataac cggcgcttcg ccttgcagat aacgattaat atcgcgcagc
     7741 aacgcctgcg gccctttcca ggtttcaaag acgacctggg ctatttcacg cggcagcggg
     7801 tagcagtggc gacgccagaa atcggcgcag gcctggtagc gtagcagaga ctcatcttca
     7861 atcagctgct gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg
     7921 caaaagccgt gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac
     7981 cactgcgcgg cttgcgcttt atcgtcgatc tcttccagca ggcgttcgta cagtggattg
     8041 tcggtggttt cacgcagaca ggcgatgcgc aactcgtgga tattgctacg gatacgaccg
     8101 cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacgatcagc
     8161 gggcggggaa aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca
     8221 atcgtaaagg ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc
     8281 aagcgcaaag gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat
     8341 tgtttgctgc aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc
     8401 ggctttcccg ttctggctgc cggaaatctg cgacagaata gccatgcctt gcggctcgac
     8461 caccgcctga tggaagaaat cagcaagttt ttgcggcgtc agcagtttta tctgggccac
     8521 gattttatca cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga
     8581 tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg gtaattatcg cctgctggat
     8641 ttgcgcaaac tcatctggct tcatcgctcg caattttgcc tctgcggttg ggaaaaacgc
     8701 cttgtaacgc tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat
     8761 gccccactga cgccccacgc tcattggaaa cgcaaacacg gcatagccca attgttcttc
     8821 ggtacgcaac tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc
     8881 tgagctggtg tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt
     8941 gctgttaccg tctttttcaa agatgacgga ttgtttttta tcgaccacta catctttgtt
     9001 tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt
     9061 tgcctgggcc tcggtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa
     9121 ggcgtcgcga taggccagca cctctttcaa cgtaatggag ggcaaaattt tacgccgttc
     9181 atctcgcgag aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcaaa
     9241 cgctttaccc ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag
     9301 ctgctcttcc gtagcggtat agctaaagta cccctcgagc aatgcctgga acagctgcgg
     9361 cagacgctgg gtgtaaccat tagcattaac cataaggccg ttgttagcgt tggtggaaaa
     9421 acttatgcca ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata
     9481 atcattgagc gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa
     9541 aatcaggctg acatcagctt tgggctcgct ggcaaaataa cggcttggcg catacaccac
     9601 gcgcagattc gactcatcaa caatcagctc tggatggtcg tatttcttct ctgacttaat
     9661 cagcgagaaa tcatcaggaa tataagggtt aagctctggc aaagagagcg caatgtcggc
     9721 ggcttttttc tgccagtcgg cgaaagtttg tgcgctgatt ttatcgacct gatacggcgc
     9781 atcgacaaag taagccgttt tgttgtgcgg ctctttcggg ctgatatacc agatacgcgc
     9841 attctgcggc gtcatcatcg ccagacgttc ctttactgct ttagcatcgt accgatcggc
     9901 aatattgact gcatccagcg tatgctcaac aggaacgcga atcatggtat ctgccagcca
     9961 ttcgacgtaa tccatatcac gggtgatcga cggataacgg aagtcgatat ccagcacatt
    10021 cgccagttca tcgaagtatt gtttatcaat gcctttttca cgtaacagat tgagatagct
    10081 aaaaattgcc gccacaacct gatcgcgatt agccaggcct ttatcggtta aagacgcaga
    10141 gatcgctaat acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc
    10201 aactaatccc tgcttttgca gccagtcaga aagtgtacct gggctgcgat tgccaatcag
    10261 ataggtaatc aattcatcgg ttttactacg gaacttcgct gagttgttat cgatgcgaaa
    10321 ctcaacgcgc aacactttac gcggcagcgc agggacgtaa tgaatgataa tgcccttttg
    10381 cgcgtcggtg actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg
    10441 accaaaggtg tccgccgcca tttttgccaa ctccggcagc ggtttattac tgtaaataac
    10501 cgccttcatc aaattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac
    10561 cggattacca ggtttgtcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg
    10621 gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg
    10681 cgccatggtt aattcagcgt tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag
    10741 caagggttct gcaatagcat cggccaggcg gtctaccgca ccgggcaagg cgtcgttctc
    10801 aacttccaga tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg
    10861 cattttgaga tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact
    10921 catatgttca aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac
    10981 gggcactacc agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac
    11041 cataccgtta tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat
    11101 ggtttcctga atcggctgcc atcccgtttc tgcctgactt aatggtgccc agagggcaac
    11161 aaacaacaat aatgctttga accaggtgct gcggggcatt cacggacctc ataatcaact
    11221 ttattttctg gacagattca acaggttaac gcaaatcatc tgccagaatt taatcttgtg
    11281 ctgcacgagt cagcctatgt ttatataacc atcagtccgt gactggtgcg catcataaag
    11341 taagcggata gattgcgcaa tttttataca acactcatga ctgattaaag cgaaacagcg
    11401 gtaacaggaa acgttgcgac tgttcaacga tggcctccat tgtctctggt gttaattgcc
    11461 gccagagccg ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt
    11521 aagcctgaag gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat
    11581 cgttttgcgc gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca
    11641 atggcgcgga cattccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct
    11701 gttcggctgc aagcggcgga aaacgccact caccgtcttt gcgtagaaaa aggcgacttt
    11761 cgccattacc accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg
    11821 ccacacttaa taaagaggga cgccagcgca acaggccatc cggctgcacc tgcggcaacc
    11881 agccagttat ctgcacaccg ttgcaggcga gatcaatttc catactctgc cccggctggc
    11941 gacaggcaat gactctgtcg gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa
    12001 tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt
    12061 cggcatcatc ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa
    12121 gtccttccag aataaatggc tcggtgtctg ggatttcgct gtcttcagta cggaagttca
    12181 cctgcaaacg catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt
    12241 ccagcggcac ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt
    12301 taccagcctg gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct
    12361 ggtagttttg cggatcaaac ggcatccggg tatggaggca agtaagatgc gcttttaccc
    12421 ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga
    12481 tgtagtcgat cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg
    12541 aacgcccgat atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca
    12601 ggtagcggtc gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt
    12661 caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa
    12721 ccactttgaa cggaattgaa cgcattggca tcagagtaca aatgttaacc ggtccggcga
    12781 gaaaacgctg gctgatacgt tcttgatcca gacgctgtgc cagttcatca cgcaatagtg
    12841 acagcggcac cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt
    12901 gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgttga
    12961 gcatatcgcg acaaaccggc aaccactctt ctagcggacg ctcctgtgcc agcccgcgac
    13021 gccagatgtt tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc
    13081 cgctcgattc atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt
    13141 agcccagcaa catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt
    13201 ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccact
    13261 ggcgtaaata acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat
    13321 ccagcagcgc cagcacatcc tctgacacaa agcgactgtc aggcagtgat aacaggctga
    13381 taaacgcttc cagtaccgga tgtgactgcc gcgcacgacg gtcggaaatg gcgtaaggca
    13441 ggtaacgatc cgcaggtgcg ctaccaaaca ctgcctgaat aaacggactg tagctgtcga
    13501 tatcagccac catcacgatg atgtcgcgcg gagtaagtgt cggggcttcc tccagcatcg
    13561 ccagcaagcg atcgtgtaaa acttcaactt cacgctgcgg gctatggcaa acgtggaatg
    13621 tgatactgct atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga
    13681 tgttcacacc cgcaacggcg cggttttcca gttccagaat gtcagactga atgttatgca
    13741 gcaggttatc tggcgtcaca tcgacaaaag cgtccagctc ctggctgctc tccaggtcag
    13801 aaaggagata aatgtagtcg cgcccaagct tcccccatga ggccagcagc gggttgccga
    13861 catcctgttc accatcgctg ttaaagagct gtccggcatt ttcgctatcg cgaaatagcg
    13921 gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgagt cagcagtttc gccagataag
    13981 caggatcttt aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt
    14041 caatatgttt acccagcgcc tggagcgcct ggagataaac aggcggtaac gcggaaatac
    14101 cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt
    14161 cgataaagcg ctgatagaga ttcgcgcggt gccagcgcgg ttgtccgagt tcatgggtat
    14221 attccaccag cgtcttccac aacggggctt gccaggcctg tgcttctccc agcccttcaa
    14281 ccaaatgtcc tgtttcccac tgtgccagcc agtccggacg atagaccaga tactggtcaa
    14341 acaggtccgc cgcttttgag gaaagctgga acagttttcg cttgtcgcta tcgtcagtca
    14401 gataatgccg caacagggta aagtcttcgc gctccagcaa ttgcggcagc agagtcatca
    14461 gtttccagct catgctctgt ttgttaaagg cgctctcttt ggggatttcc ggtaacaccc
    14521 ggacgaacat atcccagata aagctcgctg gcagcggaaa atcaatgttt gccgcaatac
    14581 caaacttttg cgacagggtc atttgcagcc actgcgccat accggtactt tgcaccagaa
    14641 tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg
    14701 cttccagcac gtccagacga ttggaatggt agacccttaa catagcggct cctgactact
    14761 gacgattcgg gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg
    14821 taacgctgat gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttgg
    14881 caggtggcga aatcgcgcgc agttgcgttt gctgccaacc atgccgccag agctgttggt
    14941 actggtttct gctggcgaga ctgttcatta atgttcgctg gatacccgat aacgcagtga
    15001 caatcatcac catcagcacc atcgcaacca ttacctccgg caggctaaag ccttgttgat
    15061 tcttcaggga agctgacata atgccccctc tttcagcgga caaaaatcgc tccagccgcg
    15121 tggcgaaaaa acaatttttc catcaatgac ttcgcctgtt cgccacaacg aaacgccttc
    15181 ataaccggca atcaataagg cttcattatc tgccagtaaa cgcaaacaaa cctgggcatc
    15241 ggttccagcg tactgcgagc actgaactgc tggctgcgtc tgccagcagt gcatttttcc
    15301 ccacgccagc gccgactgaa cgatggcctg gcggcgcaat gactgacttt ccatgctcac
    15361 gcgagaggca aaactgcgat cctgctgact cattccttgc aatagcaagc tacccaaaac
    15421 cagcagcatc aggaccagag ccagtgacga aacacctttt tcgcggttca caggttgaat
    15481 cctgtcacgc tatagctggc atccaccacg gtttgcggtt cagacttact ggcagcacgc
    15541 atattaaccg tcaacaccgg cgagaagccg ctgacatcct gacgtacgac ctgaaaagtg
    15601 tcgataatga tggcatccgg attagtgact ttatcccagc ccttaccttc acaggatgtc
    15661 gcaccgcgta gcgtttccag cacatgctcc ttcagacgaa atccaatctg gtcggactct
    15721 tttaccggtt cgcgatccca gataccgtta ctgttcgcat cccactgcac aatgatacag
    15781 tcaccctgtc cgacaatttc cagcccttcg ccggtacagc tgccatgaca ataacccgcc
    15841 ctctggagat gcttcgcgac ggtaaatacc cgcagccaga tttcatcttc cagtgccagc
    15901 ttacgagtgc tcgttaaact ttcacgctgt aacgcaggca gaaagcgtgc agcccccagc
    15961 aacaatacgc tactgatcgc catagcaatc aacacttcca gcagagaaaa accttgctct
    16021 tttacaggca tccttctgtt tctccttgct gataaagccg gagtcttccc cacggagaaa
    16081 ccaccagcca ccactcgccc gttgagtttt tgaagcgaat atgcccggcc catgcggtat
    16141 tgcgcaggcc aaagaaagca agcgaaggtg tcaggtcgct catttcgact tcgggccagc
    16201 gtggcacaaa gaccaatggt gaactgccat gacaggtatt ggccccagca gcggaactca
    16261 caaggcacca taacgtcccc tccctgataa cgctgatact gtggtcgcgg ttatgccagt
    16321 tggcatcttc acgtaaatag agcaaatagt cccgcgcctg gctggcggtt tgccaaagcc
    16381 gttgcgactg ctgccagtat tgccagccat agagtccact tgcgcttagc atgaccaaaa
    16441 tcagcatcgc gaccagcgtt tcaatcagcg tataaccacg ttgtgttttc atgccggcag
    16501 tatggagcga ggagaaaaaa agacgagggc cagtttctat ttcttcggcg catcttccgg
    16561 actatttacg ccgttgcagg acgttgcaaa atttcgggaa ggcgtctcga agaatttaac
    16621 ggagggtaaa aaaaccgacg cacactggcg tcggctctgg caggatgttt cgtaattaga
    16681 tagccaccgg cgctttaatg cccggatgtg gatcgtagcc ttcaatctca aagtcttcga
    16741 aacggtagtc gaagatggat tcgggtttac gtttgataat caacttcggc agcggacgcg
    16801 gttcgcggct taattgcaga tgagtttgat ccatatggtt gctgtacagg tgcgtatcgc
    16861 caccggtcca gacaaaatca cccacttcca gatcgcactg ctgcgccatc atatgcacca
    16921 gtaacgcgta gctggcaatg ttgaacggca ggccgaggaa gacgtcacag gagcgctgat
    16981 aaagctggca agagagtttg ccgtctgcca catagaactg gaagaatgca tggcacggtg
    17041 ccagcgccat tttatccagt tcgcctacgt tccacgctga aacaataatg cggcgcgaat
    17101 ccgggtcgtt tttcagctgg ttcagtaccg tagtaatctg gtcaatatga cgaccatctg
    17161 gcgttggcca ggcgcgccac tgtttaccat acactggccc gaggtcgcca ttttcatcgg
    17221 cccattcgtc ccagatggtg acattgtttt cgtgtagata agcaatgtta gtatcgccct
    17281 gcaggaacca cagcagttcg tggatgatgg aacgcaggtg gcaacgtttg gttgtcacca
    17341 gcgggaatcc atcctgcagg ttaaaacgca tctgatgacc aaaaatggaa agcgttccgg
    17401 ttccggtacg gtcgtttttc tgtgtgcctt cgtcgagcac tttttgcatc agttctaaat
    17461 actgtttcat ggttcctcag gaaacgtgtt gctgtgggct gcgacgatat gcccagacca
    17521 tcatgatcac acccgcgaca atcatcggga tggaaagaat ttgccccatg ctgatgtact
    17581 gcacccaggc accggtaaac tgcgcgtcgg gctggcggaa aaactcaaca atgatgcgaa
    17641 acgcgccgta gccaatcagg aacaagcctg agacagctcc cattgggcgc ggtttacgaa
    17701 tatacaggtt gaggataata aacagcacca caccttccag cagcagttcg taaagctgtg
    17761 atgggtggcg cggcagcaca ccgtaagtgt cgaaaatgga ttgccactgc gggttggttt
    17821 gcagcagcaa aatatcttct gtacgggagc ctgggaacag catggcaaac gggaagttcg
    17881 ggtcaacgcg gccccacaat tcaccgttaa taaagttgcc cagacgcccg gcaccaagac
    17941 caaacggaat gagtggtgcg ataaaatcag agacctggaa gaaggaacgt ttagtacggc
    18001 gggcgaagat aatcatcacc acgataacgc caatcaggcc gccgtggaaa gacatgccgc
    18061 cgtcccagac acggaacaga tacagcggat cggccataaa ctgcgggaaa ttgtagaaca
    18121 gaacataacc aatacgtccc ccgaggaaga cgccgaggaa gcccgcatag agtaagtttt
    18181 caacttcatt tttggtccag ccactgcccg gacgattcgc ccgacgtgtt gccagccaca
    18241 ttgcaaaaat gaaacccacc agatacatca ggccgtacca gtgaagcgcc acgggtccta
    18301 ttgagaaaat gaccggatca aactccggaa aatgcagata gctactggtc atctgtcacc
    18361 acaagttctt gttatttcgc tgaaagagaa cagcgattga aatgcgcgcc gcaggtttca
    18421 ggcgctccaa aggtgcgaat aatagcacaa ggggacctgg ctggttgccg gataccgtta
    18481 aaagatatgt atatgatccg cgctataacc ctccgcgaat cagcccgccc atgccgcgac
    18541 gctccataaa ggctgcaacc tgatgacgaa cttcggtcgc cagttgcgct tccagactac
    18601 gctgcgcaag attttctgct tcggcaaaat caatgcgccg cagcaggtat tttacccgcg
    18661 ctacagaacg tccgttcata gacagatggc gatacccaag cccaatgagg attgccacgc
    18721 acatgggatc gcccgccatt tcaccgcaca aacggagatc gataccatgt atttccgctt
    18781 cccgggcgat catcgccaga gctcgtaaca ttgcaggatg aagactgtcg taaatgttcg
    18841 ccacccgggt attgttgcga tcaacggcca gaatgtattg agtcagatcg ttggtgccaa
    18901 cagagatgaa atcgacccgc tttgccagat gcggcagcat aaataccatt gacggcactt
    18961 ccagcatgat gccgatacgt ggtttgggaa tttcgtaacc gatcatctcc tcgacttcac
    19021 gtccggcacg ttcaatcagg cggcgtgctt cgtcaacttc atcgaggctg gtgaccatcg
    19081 gcaacagaat attcaggttg cccgtagcgg cattagcacg cagcatcgcc cgcacctgga
    19141 tcaagaagat ctccggctga tcgagcgtaa tgcgaatccc acgccaaccc aggcatggat
    19201 tctcttcgct gatcggcatg taaggcagct gcttatctgc tccgacatcc agcgtacgca
    19261 aggtgacggg tttatcattg aacatttgca gcatcccctg atactgcgcc acctgttctt
    19321 cttccgacgg gaaaccactt tgcagcatga atgggatttc agtgcgataa agaccgatgc
    19381 catcaatacg gctgcccagt ttttcttcat gttccgggct taaaccagca ttgagcatga
    19441 cttttatacg ctcaccgctt tttaactgcg cgggtaaatt gacgtcatct tccgccagac
    19501 ggctaagctc aatctcttca ctaattagcc gctgatattc ttgcagcagt accggctccg
    19561 gatcgaccag caattcaccg cgatagccat caacgatcag cgtcctacga tgcagcaccg
    19621 aaggctgaat atccgcgccc atcacggtag ggatccccag cgcacgtacc atgatcgcag
    19681 catgggagtt ggctgcgcca tctcgcacga caacaccgac taagcgatcc tggggcagct
    19741 cagcaagagt tgtcgctgac agttcatctg ccaccagaat gaaacgttcc ggccaggcgt
    19801 tcggcccttg attagcgtca tcaagatgaa acagcaatcg ctgacccagc gcacgtaaat
    19861 cgccagcccg ctctttgaga tagttatcgc ttagcgcggc aaactgttca gcaaattttt
    19921 caatgaccgt ttttaccgcc cactctgcca ccgagccttt atcaacctcg gcaaacaatt
    19981 cgcgacgcag ccgggtgtcc gaaagcaggt gcgagtaaag atcgaaaata gccgccgttt
    20041 ctttttgtgc tccggcggca aagcgtttgc tgtagcggcg aaactcgttt gccgcttctt
    20101 ccagcgcccc ggtcagtcgt tcgcgttcca gagccggatc cagcgttgat gcctgataca
    20161 cctgttccat taaaggtaac gtggcatcct gccagccttc ggcaatcgcc acaccaggtg
    20221 ctgccggtaa tgcgcggatt cgcgtctggc gatattgccc aaacagggca gtcaactgcg
    20281 actgagaaag aatagctgcc atctgggtgg caagcgtcac caggaaggat tcttcacttt
    20341 cgtcatactg gcgcaactct cgttgctgta ccaccagtac accaagcaac tggcgacgtt
    20401 gaataattgg tacgcctaaa aacgcgcgga aacgttcttc ttttacggag gggatgtatt
    20461 tgaagctggg gtgcttttgc gcatctgcaa ggtttatcgg ttccgccagc ctgccaacca
    20521 ggccgacgat cccttcatca aacgcgagcg ttacagtgcg accgcgtggt tttttcagcc
    20581 cccgggtcgc catcaggtag taacaacgtc gatcatgatc ggccaggtag accgaacaga
    20641 cctcggtatc catcgcaaga cagatgtcgg taaccagaat atttaacgcc tcattcaggc
    20701 gtggtgcgct ggctaccttt tcgactattt cgcgcaggcg agtgagcata attggcgtga
    20761 cttaacctct tttacgtcga taagcagatg cgttttgtgg ttttggcgta ttttcctgca
    20821 gtgacatcac cacactcgcg aactctttca ttaccctacg gtagacatca cgtttaaatg
    20881 acaccacctg tctgaccgga taccagtaac ttacccatcg ccagccgtca aactctggtg
    20941 tactgctggt ttgcatattg atttctgcat cgccgcttac cagctgcaag agaaaccatt
    21001 tttgtttttg gccgatacaa accggcttcg tgtcccaacg caccaaacgt ttcggtaatt
    21061 tgtagcgcaa ccagttacgc gttgaagcaa ggattcgaac gtctttgcgg cttaatccta
    21121 cttcttcaaa caattcacgg tacatcgcct gctctgcgga ttctccgggg ttgattccgc
    21181 cttgcggaaa ttgccaggag tgctgaccaa atcgccgggc ccacattacc tgcccctggc
    21241 gattacaaat cacgatacct acgtttgggc ggtagccatc gtcatcaatc accggactac
    21301 ctcaaaataa agctttatat acgaatgatt gtttcatact ccaggaagac ggtaaaccac
    21361 tctctgcagg gcattacaca ctaataacaa ttgaataact cacagttatg tgcagagtta
    21421 taaacagagg aaggggtgga tagccgtttt tattcacctt ttctgtggat agagttgtga
    21481 agaactacgg aattactacg ggaaaacccg gagcattctg aataagcccg atatagacat
    21541 tttaaaatat tcattattag tcaatgcatt acatgtaatt tgagtaatga aaaagagtaa
    21601 ttcgtgaccc aggtcacacc tctcatttac gggttggcga aagatcaacc aatgccgtat
    21661 ttatccacag aatgagccac taagttaagc actgaaccac taaaaactgg agtttcgtcg
    21721 cacgtcaagg ctgtaaatga aaacagtagt ggaggttttt cacagttatc ccagctttct
    21781 gtggataaca tggtgtaaga tcctgtttat tttcagtgac cagatttgga aaacctattg
    21841 cagtgttgcg caactcgatt accggcagcc taaaaagcaa tataaatcag agaattgaac
    21901 aacacatgtg gaaaaagtta cactgcgaat attcggcaca taattgctgt ttgtttttta
    21961 accaaggtat catgacatgt cccaacctcg cccactgctc tctcctcccg aaactgaaga
    22021 acagttgtta gcgcaagcac agcaactttc tggttataca ttgggagaac tggcggcact
    22081 tgccgggctg gttacgccag agaatttaaa acgcgataag ggctggattg gcgtgttact
    22141 ggagatctgg ctaggtgcca gcgcagggag taaacctgag caagattttg ctgctctggg
    22201 cgtggaactt aaaactatcc ctgtggatag tcttggtcgt ccgctggaaa caacattcgt
    22261 ttgtgttgcc ccgttaacgg gtaatagcgg ggtgacctgg gaaaccagcc atgtgcgcca
    22321 caagctcaaa cgcgtactgt ggataccggt tgaaggcgag cgcagcatcc cgctggcgca
    22381 gcgtcgcgta ggatcaccgt tgctgtggag cccgaatgaa gaggaagacc gacagctacg
    22441 cgaagactgg gaagaattaa tggatatgat tgttctcggt caggttgagc ggatcaccgc
    22501 tcgtcacggg gaatatttac agataagacc gaaagcagcg aatgcgaaag cgcttaccga
    22561 agccattggt gcccggggcg aacggattct gacgctgcca cgcggctttt atttgaagaa
    22621 gaatttcacc agtgcactac tggcccgtca ttttctgatc cagtagccat cgctttgacc
    22681 tgccgctttc cgggcatata attaccgctt catttttttg gcagggcttt ttagatgtta
    22741 tttgcatgga taaccgatcc taacgcctgg cttgcgctcg gtacgctgac gctgctggag
    22801 atcgttcttg ggatcgacaa tattattttc ctttctctgg tggtggcaaa gcttcccaca
    22861 gcacaacgtg ctcatgcgcg ccgtctgggg ttggcgggag ccatggttat gcgtctggcg
    22921 ctgctggcat caatctcttg ggttacgcgc ctgacaaatc cgctttttac aatattcagt
    22981 caggaaattt ctgcccgtga tttgattctg cttctgggtg gcttgttcct tatctggaaa
    23041 gccagcaagg aaatccacga atccattgaa ggtgaagaag aagggctgaa aacacgcgtt
    23101 tcatcattcc tcggcgctat cgtgcagatt atgctgctgg atattatctt cagcctcgac
    23161 tcggtgatta ccgctgtggg tctgtcagat cacctgttta ttatgatggc agccgtggtg
    23221 attgccgtag gcgtgatgat gttcgccgcg cgctcgattg gtgattttgt cgaacgccat
    23281 ccttcggtaa aaatgctggc gctctctttc ctgattctgg tgggctttac cctgattctg
    23341 gaaagtttcg acatccacgt accgaaaggt tacatctact tcgcgatgtt cttctctatt
    23401 gcggttgaaa gcctcaactt gattcgcaac aaaaagaatc cgctctgata ctccgtacgc
    23461 tctcctgcag gagggcgtac tcactccttt taccctcaca gtttaagatt ttcctgcttt
    23521 caagatatat aacgtcggtt tataaacaga ctattatcat aggtaggcca aatgaggata
    23581 accggatgaa aaaatgggca gtaataattt ctgcagtcgg actggcgttt gctgtttccg
    23641 ggtgttccag tgattacgtc atggcgacca aagatggccg tatgattttg accgatggaa
    23701 aacctgaaat tgatgatgat accgggctgg tgagttatca cgatcagcaa ggtaacgcga
    23761 tgcaaattaa ccgtgatgat gtttcgcaaa ttattgaacg ttaacaaata aggtcagcat
    23821 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
    23881 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
    23941 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
    24001 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
    24061 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
    24121 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
    24181 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
    24241 ccgcgaagcg ctgcatgaca gcctcaagcg cctacagact gattacctcg atctttatca
    24301 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
    24361 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
    24421 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
    24481 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
    24541 gttaaaccgc agttttgaag taggtctggc agaagtcagc cagtatgaag gggtcgaact
    24601 gctggcctat tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa
    24661 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
    24721 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggccttg atcctgctca
    24781 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
    24841 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
    24901 attagctgaa attgaagcgg tacatcaggt ttatacttat cctgcaccat aaagattaac
    24961 gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat
    25021 tgccagcgca aatagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat
    25081 taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg
    25141 tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt
    25201 gagcggaacg acaaaaaagc cccccagcac gccaatcagc atcaacaagg cataggctgg
    25261 cagcagctcg tgttgcaggg agaaaatcag taccaccacg ccaatcaaaa tcccggctgg
    25321 catacagcgt gacacggttt ccagcgtaac cagcttcgct gccgcacctg cgccaaccac
    25381 gataccaatc gctaccatcg cgttgagata ggtcggcgta gcgttatcgg taatgcccag
    25441 cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa
    25501 actggtgccc accagcgaaa aacgcgtttc accattgcac catagcgagg tgcaggcatt
    25561 caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag
    25621 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag
    25681 ggcgaccagg acatgccagt cagccagcac accaccggct acggaaccga gtaaaatcgc
    25741 cgctatggta gaagcttcca ttaaaccgtt agcttttact aacttactac ccgtggttaa
    25801 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
    25861 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
    25921 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
    25981 cgccaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
    26041 atagaactgc gctttcagta acgccagagt ggcaaacagt agcgcgttat cgccaaacgc
    26101 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt
    26161 gtgcactgac tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa
    26221 agtcaggttt gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta
    26281 ctgcaagctc cggcacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt
    26341 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
    26401 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
    26461 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct
    26521 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
    26581 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
    26641 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca
    26701 ggcgtgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
    26761 cgttgataga aacgacaggc gcgcattcgg tcacaccgta gccttcaagg atgcgcaggc
    26821 caaatttatc ctgccaaagc tgtttggtac tttcttgtag tttttctgcg cctgccacca
    26881 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
    26941 tcgaggtacc gaacaacacg gtgcaactgc ggtcatacac caagtccggc acaatgcggt
    27001 aatgcagcgg gcttggataa aggaacactt ctgcacctgt aagcagtggc gtaaacaggc
    27061 ctaccgtcag cccaaaggag tgaaacagcg gtaatgccga cataaagcga tcgttggtgg
    27121 tgaagtcggc aatcgtttta atctgctcga cgttcgccag aatgctttta tggctatgga
    27181 cgacgccttt tggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg
    27241 gctgctgttt tacctgcgcc aggcgcggca tcagcaaatg agcgaagatc catactttgt
    27301 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
    27361 gctccggcag atgccagagt ttgcctttat cgagaaactg gcaggaagtg aagatggttt
    27421 tgatttcagc cgccgtaata gcactggtca gcccttttac cccggcagtg tagttcatca
    27481 ttgcgggaat gcggcgacgc gcgatggccc caaaaatcac tgccgcactg atgcctgcat
    27541 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
    27601 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga
    27661 cacatttctt cccggctccg aagcggtaca ttgcactcag taaagattcg tacagagttt
    27721 cacgcgggcg caccgccata cgcgcttcca tcattatttg atgcagcatt tcgccagcga
    27781 ttttgcgacg gtcacgggca cgcggcgcat ccggcatcgc cacctgcgtt ggaggcaaaa
    27841 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
    27901 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
    27961 caaaacccgc gccatcgtag attttcatca gcgagcctgt agtggtgatg cgtccttcag
    28021 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atagccatag
    28081 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
    28141 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
    28201 aaatgccatc aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg
    28261 cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
    28321 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
    28381 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
    28441 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
    28501 ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat
    28561 gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca
    28621 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac
    28681 ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc
    28741 ggtcaatcgc aacagcgttt cgcaaagtgt aaccaaaggt ttgcaatctc ttttttgtct
    28801 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccacccct tgaaccaacg
    28861 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
    28921 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
    28981 aataattcac ccaaagccag cgaagcttcc cggctggctg tgcatagtgc aatggagtct
    29041 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
    29101 ggtctgatcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
    29161 caggtggctt atcacaccgg taatttctta ttgattggca acggttacca caacgaacaa
    29221 aaagagcgtc aggctattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
    29281 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
    29341 ctgatcaacc gtatcctgcc tggctttgaa aaccgttgta ttgctcttga cgatcgttac
    29401 ggtgcctggc tggcaacgcg tcatttaatt cagcaaggtc atacccgcat tggttatctg
    29461 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
    29521 gctgaaagtg gtattccggc caatgatcgg ctggtgacat ttggcgaacc agacgaaagc
    29581 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
    29641 tgttataacg attcaatggc ggcgggcgcg atgggcgttc tcaatgataa tggtattgat
    29701 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
    29761 ccgcgcctga ccaccgtgcg ttacccaatc gtaacgatgg cgacccaggc tgccgaactg
    29821 gctttggcac tggcggataa tcgccctctc ccggaaatta ctaatgtatt tagtccgacg
    29881 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
    29941 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
    30001 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctattgta
    30061 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacctgc
    30121 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
    30181 gcccgattca cataacggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
    30241 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
    30301 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
    30361 ccgcacctga gtaattaata cgccagactg cgctaccagg aagcgacccg gttcaatttc
    30421 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
    30481 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
    30541 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgca ccatagcacc
    30601 acacacctgc tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
    30661 tagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
    30721 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
    30781 cggattaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
    30841 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
    30901 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
    30961 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
    31021 cattaagcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacttcaaa
    31081 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
    31141 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
    31201 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
    31261 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatatg gattaccgga
    31321 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
    31381 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
    31441 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggctg aaattgtttg agcgcgtacg
    31501 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
    31561 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
    31621 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
    31681 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
    31741 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
    31801 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
    31861 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaattacat
    31921 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
    31981 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
    32041 ggcgggggta ggtgtttcgg tggttaaccc gctcaccgca ctagattatg cggcaagcgg
    32101 tttagtggtg cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
    32161 gcaccgcccg tcatcagcgc tggttcaggc gtttagtggg catttacaag cggggttacc
    32221 gaaactagtc acttctcttg acgctatttt gtcgtcagct acgacagcat aaaagcgaca
    32281 gcatcctcgg catggatcgc cgcggtatca aacacaggca aaacactgcg ctcttctggc
    32341 accagtaaac caatttctgt gcagccaaaa atgacgccct gtgcgccctg ttctgcaagg
    32401 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
    32461 tcttcaaaaa taatctgatt aattttcgcc cgttcatccg cttcaggaat aagacaattg
    32521 atggaaaatt gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
    32581 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca
    32641 atgtgtaaga aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta
    32701 ttggtacata acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc
    32761 agaatgtccc cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa
    32821 tcgacgctat gtagcagcat ttgcgcagag tgaagcccac caagccgctg tttaatgcct
    32881 tcatttatca gacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca
    32941 attgttttca ttcctctctc ctgatgtgtg ttaccgcaac aaggctgaat aaggaacgag
    33001 acaaacgcct caacggccag gtgcccaatc tctattaacg aaaaaagggc cggatgtaca
    33061 gcacatccgg cccgtgaaat cagacgccga tatttctcaa cttctcgcct gccatcagtt
    33121 tgcgttcgat atgttccagc gtgacatttt tggtttccgg aatgagccag aaagtaatgc
    33181 ccacaaacgc aatgttcagc gcagtgtaga gccagaacgt accggcagcg ccaatgctat
    33241 caagcagtgt caggaaggtc gcgccgataa tcatattcga cacccagttt gtcgtcgtcg
    33301 aacaggtaat accgaaatcg cggcatttca gcggctgaat ttcagagcac aggatccaca
    33361 ccactggcgc ggcgctcatc gcataaccgg caatacacat catcgtcatg ccaacagaga
    33421 gccaagacaa gccactggaa gccgtaccgt tatcaaactg catcaggcaa tagcccagca
    33481 ccagagtgcc taacgccatc acgctgaaac caattttcag agccggttta cgacctgctt
    33541 tatctaccgt aaagaccgca atgaaggtcg cgaacataaa ggtcagtccc acgaccagag
    33601 tcgcaatcat ctgttgttct gtggtcgtaa agcccgccat tttgaagata cgcggcgcgt
    33661 agtacatgat gatgttcata ccggtaaact gctgcatcgc ctgcaacaac ataccgagaa
    33721 acacagcacg acggacgtta cggttgatct taaacagtgc ccaaccgccc tgttttaact
    33781 tcaggctttc acgaatttcg ttgagttctt ctcgcgcttt ttccgacgta tcgcgcagca
    33841 tacgcaacac ttcttccgcc tcaatatgac gccccttttc cgccagccag cgcgggctat
    33901 ttggcaggaa gaccaccaga ataatcagca gaactgctgg taaagcaaga acccccaaca
    33961 ttgcgcgcca gttaccgcta taactgaacg ctgtatcgga taaaaacgcc agcacgatgc
    34021 cgagtgtgac catcaactgg tacatactga tcatcttacc gcgaacgttt tcacttgcca
    34081 tttcagaaag atacagagga gcggtgtaag acgcgatccc gacagcaatg cccagcacca
    34141 cacgagcggc gattaacatc tctacgctgg tcgcaaaagc ggaccctata gaaccgagta
    34201 caaacaggat ggcccccgcc atcaggctgt atttacgccc caggcggaac gacagccaac
    34261 cattaaacag cgcaccaatt gctgcgccga gcatcatgct actaaccacc cattcctgca
    34321 aacgactggt cagcacaaag tgatcggtaa tgaacggcaa cgctccggcg attacgccga
    34381 tatcaagacc aaataacaat cctgcgaccg cagcagctac cgaaacaaac atattcatac
    34441 gccgcgtatc acgcaaagaa cgtggcgtta aagcagattc cgtattgata gtaaccattt
    34501 tttcctgcca gcagagagta agacatagtg aaaaaatacg tgaacaattc acacaggtgt
    34561 caggtcggaa acagcataaa tatggattaa attgctgcga catgccgtta tgtgatggat
    34621 attccaattt taaaattaag ttgaattatt gagattatta ttaaccacct aattttaaag
    34681 cagataaaat tcataaagtt cattaattga taattgatat ggattatgat gatctagaga
    34741 tataaaaaac cctgccatgc ggcagggtca taaaagtaag aggaatgaat taacgcgcca
    34801 gccaaccgcc atccacagca atggtataac catttacata atctgaagcg ctggaggcaa
    34861 gaaacactac cggccccatc aggtcactcg gcagtcccca acggccagct ggaatgcgat
    34921 cgagaatttc cgcgctacgt tgttcatctg cccgcagttg ttgggtattg ttggtcgcca
    34981 tgtaacccgg agctatcgca ttaacattaa tgttgtgttt agcccattcg tttgccatca
    35041 atcgcgtcac acccatcacg ccgctttttg atgcggtata agaaggcaca cggatcccgc
    35101 cctggaagga gagcattgac gcgatattga taatcttgcc gccattgcct tgcgcgataa
    35161 agtgtttcgc cgctgcctga gacatgaaga atacgctctt gatattcagg ttcatgacat
    35221 cgtcccagtc tttttcgctg aactcgagag cgtcttcgcg gcgaatcaat ccggcgttat
    35281 tcaccaggat atcaatatga ccaaattcag ctaccgcgcg atccagcagt ccaggaatgc
    35341 catcaatctt tcgcagatcg gcggtcaggc ttaaaaaacg acgccccagc gccgtgacct
    35401 gctcgatggt ttcagtcggt tcaacgatgt taatgccaac aatgtcacag cccgcttgcg
    35461 ccagccccaa cgccatcccc tggcccagcc cagtatcaca accagtgacg accgcaactt
    35521 taccttggag agaaaatgca cttaaaatca taacaacacc ttattcgttt atgcccacaa
    35581 ctagcgcaaa tctttaacgg ccacatggtc catatcatta aagacctggt tttcaccgac
    35641 catgccccag ataaaggtat aagctttggt tccgacgccg gaatggatcg accagctcgg
    35701 ggagatcacc gcctgctcgt tatgcatcac aatgtgacgc gtttcttgcg gctgccccat
    35761 catgtggaaa acgcaggcgt catcatccat attgaaatag aaataaactt ccatccggcg
    35821 ctcgtgggtg tgacacggca tggtgttcca caagttacct ggagccagct ccgtcagccc
    35881 catactcaat tgacaggttt ccagtacatc cgggacaaaa tatttgttaa tcgtgcgacg
    35941 gttactggtg aggttatcgc ctaacgtgac tggagatact tcgtccggtg tgactttttt
    36001 ggtgggatac gtcgtatgcg cgggtgcgca attgtaataa aacttcgccg gagtggcggt
    36061 atcaatactg gcaaagacaa cttcttttgc acctttacca acatacaggg cgtcgcggtg
    36121 accgatttca tagcattgac catcgacagt aatcgtaccg gcaccgccaa tattgataac
    36181 acctaactcg cgacgttcaa ggaaatagct tacgccgagt tgtttaccaa cttccccgcc
    36241 aacggaaacc gttttagtta tcggcataat gccgccaaca ataattcggt caatgtggct
    36301 gtaaaccatg gtgtactcat cggcgacaaa taccttttca accaaaaatt cattgcgcag
    36361 cccttgggta tccagcgttt ttgcgtgcgc accgtggatg ctctgtctta cgtccacatc
    36421 aacctccgat aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac tgtttcgttt
    36481 tctctataaa gggatgatat gcccatgggc tagggttttc aattaaaaat aaaacaatgt
    36541 ttcattttta agttgaggat taaaaaaagt gcagtgtcga tcacgaatat gtcatgagaa
    36601 tggaaaaatg agggagccca gaaaatgtat gtcgacttgc cagccatcat tattcaacag
    36661 attaatgacg gcagatattt atctgtaagg aggaaatgca gagctactcg tcacgttcaa
    36721 tggtcaatgc cacgccctgg cccccgccga tacaaagcgt tgccagccct ttgcgggcat
    36781 tacgtttcac catttcatga accagagaaa ccaggattcg gcaaccggaa gcgcctatcg
    36841 ggtgaccgag tgcgatcgcg ccaccattga cattgacccg acgctcatcc cactcaagca
    36901 tcttgccaac cgaaagcgcc tgtgcagcaa ccgcttcatt agcctcgata agatcgacat
    36961 cagccaactg ccagcctaca cgctccaggc aacggcgggt cgcatacacc ggcgcaattc
    37021 ccatcaatgc ccaccaacgc tggcaaatgc gcggatgcgg gccagcacgg gtaaattcaa
    37081 cgctcgagct ttggcttcgc tcatcatcat taccgcagct gcgccatcgt ttatggatga
    37141 tgcattcccc gcggtcacag aaccgagact atcaaatgaa ggatttaaac gggctaaacc
    37201 ttctgcactg gcgtcagtgc gtggctgttc atcggtatca acaaccaacg tctgaccgtt
    37261 actttggctc attaccggga cgatctcatc tttaaatcgt ccggcgtcaa tcgccgctcg
    37321 cgctttttgt tgcgagctaa gtgcgtaagc atcctgcaac tgacggctga tgccatattc
    37381 gcgagccaga ttttcggcgg tgacaccaat atgataatca ttgaaggcat cccacaaccc
    37441 atcatgcacc agactgtcaa ccaactggct attgccaagc tgtgcaccgg tgcggctatc
    37501 agtcagaaca tgtggtgcgc ggctcatgtt ttcctggcca ccggcgatga caatatcagc
    37561 ctcgccacac tgtatcgcct gagtagccag atgcagtgct ttaagaccgg aaccgcaaac
    37621 gtcattaatc gtgattgcag aaacgctgtt aggaagacca cctttaatag ccgattgcct
    37681 tgccggattc tgccctgccc ctgcagtcaa cacctgacca agaattactt catccaccgc
    37741 atatgcagga acgccggtac gttctattaa cgctttcacg accagactac caagttccac
    37801 ggcagaatga cccgctaacg caccacgaaa gcagccgata ggtgtccgta acgccccgac
    37861 aatcacaacg tctttcatca caacctcgca ccaattaaca gtgcaatagt aaatgattgt
    37921 taattacagt tatcttaatt gtttaaaaaa agtgattttt atcacaaagg aaatatgcct
    37981 gagcagtagt cagagtcata actggcacgt aaggtttgca accactaacc caccaataga
    38041 tgggtagata gggcgttaat ctcccatact taacttggtt tatggtaaat tgccctccat
    38101 tttgtttaat ttgtagatga tacgttcaga taatgtctaa tatttggtct aaagaagaaa
    38161 ctctgtggag tttcgcgctc tacggcacag ccgttggtgc aggcacgcta ttccttccta
    38221 ttcagttagg ttcggcaggg gctgtggtcc tgtttattac tgctctggtc gcctggcctt
    38281 taacatattg gccacataaa gccttatgcc agttcatcct ctcatcaaaa acatcagcag
    38341 gtgaagggat aacgggcgcg gtaacacact actatggcaa gaagattggt aatctgatta
    38401 ccacgctgta cttcatcgcc ttttttgtcg tcgtgttgat atatgcagtg gcaattacca
    38461 actcacttac ggaacagctg gcaaagcata tggttattga tctttgcatc cgtatgttgg
    38521 tgagtctggg tgttgtatta attctgaatc tcatttttct gatgggacgt catgccacta
    38581 ttcgggtaat gggatttttg gtattcccat tgattgccta tttcttattt ctttccattt
    38641 accttgtcgg tagttggcaa cctgatctat taacaaccca ggtagagttc aatcagaata
    38701 cccttcacca gatatggata tcgattcccg tgatggtttt cgcctttagc catacgccca
    38761 ttatttctac gtttgccata gacagacgtg aaaaatatgg cgaacacgct atggataaat
    38821 gcaaaaaaat tatgaaagtc gcttatctca tcatctgcat aagtgtactg ttctttgtct
    38881 ttagctgcct gctttctatt ccatcttcgt atattgaagc ggctaaagaa gaaggggtca
    38941 ccattttatc ggcgctttct atgctgccga acgccccagc atggttgtca atttccggga
    39001 ttattgtcgc agtagttgcg atgtcgaaat cattcctagg tacgtacttt ggcgttattg
    39061 aaggtgccac agaggtcgtc aaaacaacac tacagcaggt tggtgtaaag aaaagtcgtg
    39121 catttaacca cgcactatca attatgttgg tatcgctgat taccttcatt gtttgttgca
    39181 ttaacccgaa cgcgatttcg atgatttacg cgatcagcgg cccgctcatt gccatgatac
    39241 ttttcatcat gcctacgctg tcaacgtatc tcatcccggc gcttaaaccc tggcgttcca
    39301 tcggaaatct gattacgctg atcgtgggta tcctgtgcgt atcggtaatg ttctttagct
    39361 aatatccgtt gtggatggtt caaaa
//
LOCUS       DADLSA010000022        38023 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.022, whole genome shotgun sequence.
ACCESSION   DADLSA010000022 DADLSA010000000
VERSION     DADLSA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.022"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(21..1019)
                     /gene="fbp"
                     /locus_tag="JN691_001154"
     CDS             complement(21..1019)
                     /gene="fbp"
                     /locus_tag="JN691_001154"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAY7242650.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1195..2568
                     /gene="mpl"
                     /locus_tag="JN691_001155"
     CDS             1195..2568
                     /gene="mpl"
                     /locus_tag="JN691_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAY7242651.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2651..3202)
                     /locus_tag="JN691_001156"
     CDS             complement(2651..3202)
                     /locus_tag="JN691_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAY7242652.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3296..4648
                     /gene="pmbA"
                     /locus_tag="JN691_001157"
     CDS             3296..4648
                     /gene="pmbA"
                     /locus_tag="JN691_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAY7242653.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKVPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4831..5217
                     /gene="cybC"
                     /locus_tag="JN691_001158"
     CDS             4831..5217
                     /gene="cybC"
                     /locus_tag="JN691_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HAY7242654.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLADNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5262..5726)
                     /gene="nrdG"
                     /locus_tag="JN691_001159"
     CDS             complement(5262..5726)
                     /gene="nrdG"
                     /locus_tag="JN691_001159"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAY7242655.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5884..8022)
                     /gene="nrdD"
                     /locus_tag="JN691_001160"
     CDS             complement(5884..8022)
                     /gene="nrdD"
                     /locus_tag="JN691_001160"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAY7242656.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFAGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8416..10071)
                     /gene="treC"
                     /locus_tag="JN691_001161"
     CDS             complement(8416..10071)
                     /gene="treC"
                     /locus_tag="JN691_001161"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAY7242657.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPCFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNGAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10121..11542)
                     /gene="treB"
                     /locus_tag="JN691_001162"
     CDS             complement(10121..11542)
                     /gene="treB"
                     /locus_tag="JN691_001162"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HAY7242658.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11661..12608)
                     /gene="treR"
                     /locus_tag="JN691_001163"
     CDS             complement(11661..12608)
                     /gene="treR"
                     /locus_tag="JN691_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAY7242659.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12793..12846
                     /gene="mgtL"
                     /locus_tag="JN691_001164"
     CDS             12793..12846
                     /gene="mgtL"
                     /locus_tag="JN691_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAY7242660.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12987..15683
                     /gene="mgtA"
                     /locus_tag="JN691_001165"
     CDS             12987..15683
                     /gene="mgtA"
                     /locus_tag="JN691_001165"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAY7242661.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15889..16275)
                     /gene="ridA"
                     /locus_tag="JN691_001166"
     CDS             complement(15889..16275)
                     /gene="ridA"
                     /locus_tag="JN691_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAY7242662.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16348..16809)
                     /gene="pyrI"
                     /locus_tag="JN691_001167"
     CDS             complement(16348..16809)
                     /gene="pyrI"
                     /locus_tag="JN691_001167"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAY7242663.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16822..17757)
                     /gene="pyrB"
                     /locus_tag="JN691_001168"
     CDS             complement(16822..17757)
                     /gene="pyrB"
                     /locus_tag="JN691_001168"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAY7242664.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17761..17895)
                     /gene="pyrL"
                     /locus_tag="JN691_001169"
     CDS             complement(17761..17895)
                     /gene="pyrL"
                     /locus_tag="JN691_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAY7242665.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18176..18571)
                     /locus_tag="JN691_001170"
     CDS             complement(18176..18571)
                     /locus_tag="JN691_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY7242666.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18703..19416)
                     /gene="bdcA"
                     /locus_tag="JN691_001171"
     CDS             complement(18703..19416)
                     /gene="bdcA"
                     /locus_tag="JN691_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7242667.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGTLDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPIRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19487..20080
                     /locus_tag="JN691_001172"
     CDS             19487..20080
                     /locus_tag="JN691_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7242668.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20225..20677
                     /locus_tag="JN691_001173"
     CDS             20225..20677
                     /locus_tag="JN691_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY7242669.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20800..21617
                     /locus_tag="JN691_001174"
                     /pseudo
     CDS             20800..21617
                     /locus_tag="JN691_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036420.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21732..22517
                     /locus_tag="JN691_001175"
     CDS             21732..22517
                     /locus_tag="JN691_001175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242670.1"
                     /translation="MINLSHILIDSLDDAKVNLTPVIHSMLVTFLELPYNNDVKILEW
                     CFNKSMQYFDDSAKIEHACSVINHINFLCDQSKVAETLFFNLDKEPYKNSPELQELIW
                     KKLVVYVNDFNLSNREKTYLIQRIFNNVESLFNKVPVSILVNDILLNDFFMKNTEMIN
                     WYFPRLLKSYEGEKNYFDNLKYDINDEKSNKEILKNQPDNVIKEKLNNEFKLRFRMMQ
                     TIFKSKVNVSPYIDQQRLNTLNPPENLRMAIEKFGWKKKTITA"
     gene            complement(22570..23583)
                     /gene="argF"
                     /locus_tag="JN691_001176"
     CDS             complement(22570..23583)
                     /gene="argF"
                     /locus_tag="JN691_001176"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAY7242671.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
     gene            23745..24161
                     /gene="rraB"
                     /locus_tag="JN691_001177"
     CDS             23745..24161
                     /gene="rraB"
                     /locus_tag="JN691_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAY7242672.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(24207..24710)
                     /locus_tag="JN691_001178"
     CDS             complement(24207..24710)
                     /locus_tag="JN691_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7242673.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            24903..26099
                     /locus_tag="JN691_001179"
     CDS             24903..26099
                     /locus_tag="JN691_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HAY7242674.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMK
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            complement(26153..29008)
                     /gene="valS"
                     /locus_tag="JN691_001180"
     CDS             complement(26153..29008)
                     /gene="valS"
                     /locus_tag="JN691_001180"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAY7242675.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENHESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(29008..29451)
                     /gene="holC"
                     /locus_tag="JN691_001181"
     CDS             complement(29008..29451)
                     /gene="holC"
                     /locus_tag="JN691_001181"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAY7242676.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(29585..31096)
                     /gene="pepA"
                     /locus_tag="JN691_001182"
     CDS             complement(29585..31096)
                     /gene="pepA"
                     /locus_tag="JN691_001182"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAY7242677.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRARFNGEE"
     gene            31363..32463
                     /gene="lptF"
                     /locus_tag="JN691_001183"
     CDS             31363..32463
                     /gene="lptF"
                     /locus_tag="JN691_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY7242678.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            32463..33545
                     /gene="lptG"
                     /locus_tag="JN691_001184"
     CDS             32463..33545
                     /gene="lptG"
                     /locus_tag="JN691_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY7242679.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(33664..35166)
                     /locus_tag="JN691_001185"
     CDS             complement(33664..35166)
                     /locus_tag="JN691_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAY7242680.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(35295..36314)
                     /gene="ahr"
                     /locus_tag="JN691_001186"
     CDS             complement(35295..36314)
                     /gene="ahr"
                     /locus_tag="JN691_001186"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAY7242681.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            36510..36594
                     /locus_tag="JN691_001187"
     tRNA            36510..36594
                     /locus_tag="JN691_001187"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36544..36546,aa:Leu,seq:caa)
     gene            36793..38023
                     /locus_tag="JN691_001188"
                     /pseudo
     CDS             36793..38023
                     /locus_tag="JN691_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
BASE COUNT         9137 a         9445 c         9796 g         9645 t
ORIGIN      
        1 gtgtggagag gcgcaggaga ttacgcgtcc gggaactcac ggataaagcg ttcgacatct
       61 tcaaccatat ggtcgttgcc gacaaagaac gaacggcgct ggtgcagagt ttccgggatg
      121 atatccagaa tacgctcttt gccatcgctc gctttaccgc ccgcttgttc cgccaggaat
      181 gccatcgggt tgcactcata aagcaaacgc agtttgccgt ccgggtggct ggcggtgctt
      241 gggtagagat aaataccgcc tttcagcagg ttacggtgga aatccgcaac cagtgaaccg
      301 atataacgcg aggtatacgg gcgattggtg gatttatctt cttcctggca gaatttaatg
      361 tacttcttca ccccgttcgg aaacttaatg tagtttcctt cgttgatgga gtaggttttg
      421 cctttctccg ggaagcgcat ccgttcctgg cacaggcaga aaacgcccag cgaaggatcg
      481 taagtaaagg cgtgaacacc gcatccggtg gtgtaaacca gcatggtaga ggagccgtat
      541 accacgtaac ctgccgcaac ctgtttgtta ccaggctgga ggaaatcttc ttccgttacc
      601 ggcgtgccaa caggcgtaac gcggcggtag atggagaaaa tggtaccgac agagacgtta
      661 acatcgatgt tggacgagcc atccaggggg tccatcagca ccacgtattt tgcgtgttca
      721 cagccttcaa agacgacaat ctcatcttct tcttcagagg caatccccgc aacgatatcg
      781 cgtgctttca gtgcggcttt cagtttttca ttagcgaaca agtcgagttt ttgctgaacc
      841 tcgccctgca cgttctcagc accgctggca cccaggatat caaccagtcc tgctttgttg
      901 atatcgcgat ggataatctt ggcgcccagt tttattgccg acagcaaagc agtgagctca
      961 ccggtagcat gagaaaactc gtgctgcttt tcgacaataa attcacctaa cgttttcata
     1021 aaactttccc tgcaatgttt atggagtaaa gcgaccgcaa caatcttaac aaataatctc
     1081 aatgttgcgc tcaggtgaat cgcgccagca aattgcggat tatcctgaaa tgcgtttctc
     1141 acttgcccga catatgcgta aaatgagcgg cagattaaaa aaggatagtg acgtatgcgc
     1201 attcatattt taggaatttg tggcacattt atgggcggtc tggcgatgct ggcgcgccag
     1261 ttaggccatg aagtaacggg ttcggacgcc aatgtgtatc cgccgatgag caccttactt
     1321 gagaagcaag gcattgagct gattcagggt tacgatgcca gccagctcga gccgcagccg
     1381 gatctggtga ttattggcaa cgccatgacc cgtggaaatc cgtgtgtgga agcggtactg
     1441 gaaaaaaaca tcccttatat gtcaggtcca cagtggctgc acgattttgt gctgcgcgac
     1501 cgctgggtgc tggccgttgc cggtacacac ggcaaaacca ccaccgcggg aatggcgacc
     1561 tggattctgg aacagtgtgg ttacaaaccg ggatttgtga tcggcggtgt gccggggaac
     1621 tttgaggttt cggcgcgtct gggcgaaagc gacttctttg ttatcgaagc ggatgagtac
     1681 gactgcgcct tcttcgacaa acgctctaaa tttgtccatt actgcccgcg tacgctgatc
     1741 ctcaacaacc ttgagttcga tcacgccgat atctttgacg acctgaaagc gatccagaaa
     1801 cagttccacc atctggtgcg tatcgttccg gggcagggcc gtattatctg gccggaaaat
     1861 gacatcaacc tgaaacagac catggcgatg ggctgctgga gcgagcagga gctggtgggt
     1921 gagcagggtc actggcaggc gaaaaagctg accaccgatg cttccgaatg ggaagttttg
     1981 ctggatggcg aaaaagtggg cgaagtgaaa tggtcgctgg taggcgaaca taatatgcac
     2041 aatggcctga tggcgattgc agcggctcgc catgttggtg tagcgccggc agatgccgct
     2101 aacgcgctgg gttcgtttat taatgctcgt cgccgtctgg agttgcgtgg tgaagcgaat
     2161 ggcgtcacgg tatatgacga ttttgcccat cacccgacgg cgattctggc aacgctggcg
     2221 gcgctgcgtg gcaaagttgg tggtacggcg cgcattattg ctgtgctgga accgcgctcg
     2281 aataccatga aaatggggat ctgcaaagac gatctggcac cttcattagg tcgtgccgat
     2341 gaagtcttcc tgctgcaacc ggcgcatatt ccgtggcagg tggcagaagt ggcagaagcc
     2401 tgcgttcagc ctgcacactg gagtggcgat gtggatacgc tggcagatat ggtggtgaaa
     2461 accgctcagc ctggcgacca tattctggtg atgagcaacg gcggttttgg tgggatccat
     2521 cagaaactgc tggatggtct ggcgaagaag gcggaagctg cgcagtaatt cggcttcagc
     2581 ctgatatagc attgccggat aaggcgttta cgccgcatcc ggcaaaccat actcacatca
     2641 acaacgaaaa ttacccttca ttctccgcca gttcgcgcag atactggaaa atctggcgtg
     2701 cggatttcgg cggcttattc ccttctttct ctttcttcgc gttgcggatc agtgtacgca
     2761 gttgctgacg atcggcatcg ggccacagat tcaatacttc agcgatggcg tcatcacctt
     2821 gatcgatcag acgatcgcgc aggttttcca gtttatggaa cagcaccacc tgctggttgt
     2881 gacggttttt cagcttatcc agcgcctgac gaataggctc tacgtcgcgc tggcgtagca
     2941 tcttaccgat gagctgcaac tggcggcggc gaccttccat cttaatacgc tgggcgagct
     3001 caatagccgg gcgcagatcc gcatctaacg ggatcttatc cagcgcgttt ttccccagat
     3061 caacaatttc cgcgccaagg cgttttagct cctcggcatc acgtttaatt tcacttttac
     3121 tgacccagat aatttcatcg tcttcgtctt cgatgtcatc accgggaacg tcgtcgagcc
     3181 agtcttcggg ctgcttagtc atctcaggct ccttaaaaaa agaggctaat gttaccagtt
     3241 aagatgcgca ctgaaaaacg gttctctgtt agacttcaga gaaactctct acattatggc
     3301 acttgcaatg aaagtaatct ctcaagttga agcgcagcgc aagattctgg aagaagcagt
     3361 ttccactgcg ctggagttgg cctcaggcaa atcggacggt gcggaagttg ccgtcagcaa
     3421 gaccaccggc attagcgtaa gcacgcgtta tggtgaagtg gagaatgttg aattcaatag
     3481 tgatggcgcg ctggggatca ctgtttatca ccagaaccgt aaaggtagcg catcgtctac
     3541 cgatttaagc ccgcaggcca ttgcccgcac cgtacaggcg gcgctggata ttgcccgtta
     3601 tacctcgcca gatccctgtg ccggcgtggc agacaaagag ctgctggcct ttgacgcacc
     3661 gaatctcgac ttgttccacc ctgcggaagt ttccccggat gaagccattg aactggcggc
     3721 ccgcgcagaa caggcggcat tgcaggcgga caaacgcatc accaataccg aaggtggcag
     3781 ctttaacagc cactacggtg tcaaagtttt tggcaacagc cacggcatgt tgcagggtta
     3841 ctgctcaacg cgtcattcgc tctccagctg tgtaattgcc gaagaaaatg gcgatatgga
     3901 gcgtgattac gcctacacca ttggccgcgc gatgagcgat ctgcaaacgc cagagtgggt
     3961 cggggccgac tgtgctcgcc gtactttatc gcgcctgtca ccgcgtaaac tctccaccat
     4021 gaaagtgccg gtcatttttg ccaatgaagt ggcaaccggg ctttttggcc atctggtggg
     4081 ggcgatagcg ggtggctcgg tttaccgtaa atctaccttt ctgctggatt cgctgggtaa
     4141 acaaattctg ccggactggc tgaccattga agagcatccg catctgctga aagggctggc
     4201 gtcgacgcca ttcgacagcg aaggtgtgcg caccgagcgt cgcgatatta ttaaagatgg
     4261 catcctgact cagtggctgc tgaccagcta ctcggcgcgg aaactggggc tgaaaagcac
     4321 cggacatgcg ggcggtattc acaactggcg gattgccgga caagggctaa gcttcgaaca
     4381 aatgctcaaa gagatgggca ccgggctggt ggtgacggag ctgatgggcc agggcgtgag
     4441 tgccattacc ggtgattatt cccgtggtgc agcgggcttc tgggtagaga atggcgaaat
     4501 tcagtatccc gttagtgaaa tcaccatcgc aggtaattta aaagatatgt ggcgcaatat
     4561 tgtcaccgtc ggtaacgata ttgaaacacg cagtaatata cagtgtggtt ctgtgctgtt
     4621 gccggagatg aaaatcgccg ggcagtaaaa aactggcgcg acctgccgcg ctataaacta
     4681 aaaaattaca caaaatcatt cgcactgcat cgagacggaa agtgaatgag ggccggggag
     4741 cgtactcgca gtacgtgacc cgggcgaatg agcgtagccg tcgatgaggc agtgtgaagg
     4801 atgaagtgta aataaaaaag gaagtgagca atgcgtaaaa gcctgttagc tattcttgca
     4861 gtctcctcgt tggtattcag ttctgcgtcg tttgccgctg atcttgcaga caatatggaa
     4921 accctcaacg acaatttaaa agtgatcgaa aaagcggata acgcggcgca agtcaaagac
     4981 gcgttaacga agatgcgcgc cgcagcgctg gatgcgcaaa aagcaacgcc gccgaagctc
     5041 gaagataaat caccggacag cccggaaatg aaagatttcc gccacggttt cgacattctg
     5101 gtcggtcaga ttgacgacgc gctgaagctg gcaaatgaag gtaaagtaaa agaagcgcag
     5161 gctgctgcag agcaactgaa aacgacccgc aacgcctatc accagaagta tcgttaattc
     5221 ctcatttccc tgttgcctgc actcaggtaa cagggaactt atcatcgcaa atgatgcacc
     5281 acctgattgc tgctgccgcg ccagatcagg gaagggtctt ttaaatcctg cacaaatttg
     5341 ccgtcgacca gcacgttaat caaatcaaca acctgcattt gcgcagcgtt gagttcgtcg
     5401 agtttatagc ctgtccacac ccagatgtct ttacccggac actcggcgcg gatgcgtttt
     5461 accagtttca gaatatccgg cacgttttgc ggatgcagcg gatcgccgcc ggagagggaa
     5521 atcccctggc gtttgatacg agtgtcattc agatctttaa tgatcttgtc ttccattgct
     5581 ttggtaaatg gctgaccaga atttacccgc catgtgcttt tgttatagca accggggcat
     5641 tcatgaacac acccggagac aaacagggtg cagcgagtgc cggggccgtt gacgatgtcg
     5701 acaggatagt actgatgata attcattatt tcgcctggct tatcagtccg cgtgtaggct
     5761 gcatccggta atcaatgcct gatgcgacgc tgtcgcgtct tatcaggcct acagccgttg
     5821 ccaaatgtag gccggataag gcgtttacgc cgcatccggc atttgcttag cgcagagtga
     5881 agattaacct atctgcccat tccccaaatg tttaacgcgg cgcttaactt cttcctgctt
     5941 accagcgtta aacggacgtg catccgggct acctaaatat ccgcacacgc ggcgagttac
     6001 cgacacacgg gaggcgtcat ggttaccaca tttcgggcaa gtgaagcctt tgctggtgca
     6061 ctcgaactca ccggtaaagc cacactcgta gcactcatca atcggtgtat tggtgccgta
     6121 atacggtaca tgctgatagc tgtaatccca gacatcttcc agcgctttca ggttgtgctg
     6181 aatgtttgga tactcgccgt agcaaatgaa accaccgttc gccagcggcg ggtaaggcgc
     6241 ttcaaagtcg atcttgtcgt acgggttcac cttcttctcc acatcaaggt ggaaactgtt
     6301 ggtgtagtaa cctttatcgg tcacgcccgg caccacgcca aactcagcag tatcgagacg
     6361 gcagaagcgg tcgcacaggt tttcactcgg cgtgctgtag agactgaaac cataacccgt
     6421 ttcttctttc cactgatcca ctgcctgacg cagacgttca acaatggcga tacctttcgc
     6481 gcgaagcttc tcgttgtcgt aaacatgctc gccggcgaac agcgcgttaa tggtttcgtg
     6541 gatgccgatg taacccagcg aaatagacgc acgaccgttt ttgaagattt cagaaacatc
     6601 atcgtcagca ttcagacgta cgccacaagc accttccata tagaggatcg gggcgacgcg
     6661 cgctttcacg ccttcgagac gagcgatacg ggtcatcagt gccttacgtg ccagcaccag
     6721 acgttcatcc agcagcttcc agaaggtggc ttcatcgcct tttgcttcca gagcaatacg
     6781 cggcaggttc aggctgatca cgccgaggtt gttacgacca tcgtggatct gctcgccgtt
     6841 ttcattttcc cacacgccga ggaagctgcg gcagcccatc ggggttttaa acgaaccggt
     6901 gactttcact acctgatcgt agttcaggat atccggatac atgcgcttgc ttgcgcactc
     6961 cagcgccagc tgtttgatgt cgtagttcgg atcgcctttt ttatggttca ggccatcgcg
     7021 aatcgcaaac accagtttcg ggaacaccgc agttttacgg tttttaccga ggcctgcgat
     7081 acggttacgc aggatcgatt cctgaatcag gcgcgattcc cagctggtgc ccagaccaaa
     7141 accaaaggtt acaaacggcg tctgaccgtt ggcggtgtgc agggtgttta cttcgtactc
     7201 cagcgactgg aaggcatcgt agcactcttt gatggtacga gagttagcgt agccttcggc
     7261 gtccgggatg ttccactctt ctgcggtctt gcgatgtttg ttgtagctgg cggtaacaaa
     7321 cggcgccagc acttcatcga tacggttaat ggtggtgccg ccataaatat ggctggcgac
     7381 ctgagcaata atctgcgcag ttaccgcggt tgccgtagag atcgacttcg gcggttcaat
     7441 ctcggcgttc cccattttaa agccctgggt cagcatgcct ttcaggtcga tcaacatgca
     7501 gttgaacatc gggaagaacg gcgagtaatc gagatcgtga tagtgaatat cgccacgctc
     7561 atgtgcctgc accacgtcac gcggcagcag gtgctggcgt gcatagtgtt tagccacgat
     7621 cccggccagc aggtcgcgct gggttggaat caccttgctg tctttgttgg cgttttcgtt
     7681 gagtaacgag gcgttggtct gctcgaccag accacggatc tcctggttca ggcgaccgcg
     7741 tttttcacgt tcaatgtcgc gatcgtgacg gtactcgatg taagcacgag ccagttgttt
     7801 gtatggaccc gacatcagct ggttttcaac tgcggtctgg atctcgttga tatccacctg
     7861 gttgcggccc tgcatctgct cgctgacaac cgcggcaaca gtggcgcaat aatcggcatc
     7921 atcgacttcc gctgctttag ctgcacgcag aatcgcttct ttgatgcgct ctgatttaaa
     7981 cggcactttg cagccgtctc gtttcatcac atgcggtgtc atgatcgctc catattttta
     8041 agaacaggtt atccacagaa attgggaaag gcgttttccg gttttttcgt ttcgttttcc
     8101 ggtgctttcc gcaatcgcca tccgcttatc cacaacgccg cacctttttc gtggggagct
     8161 gtagtagcaa ttatagtcga ttaatacaac atattgggtt gggacgcatt ttaaagtcta
     8221 tatatagtgc tttgcatcaa ggatgtttta actttttttg atgtagctca aagtaaaaag
     8281 cagagcgtac ggatgacggg cgctacagcg ttatgtaaat tttttaatga atttgctggt
     8341 tgaaaaatca acaaaaacaa catgctgaca gacaaaaccc cggaatgacc ggcattatcg
     8401 gggcttaggg aagatttact tctgtaacca ccagacagcc tcaaaaggcc gtaaattcat
     8461 ggcacagggt tgtggtgagg cttcttcgta gttatgcatc acaagctgcc agttgccgcg
     8521 catttgccct ggctgccagg gttggatctc acggctaagg ttggcaatga ccagcaaggt
     8581 ttgccccttc cattcacggc gatagcacca caatacaggg ctgtttggca gcagatcctg
     8641 gtaattgccc catgtcagga tggcttcttg cttacgcagt gcgattaact tttggtaggt
     8701 gtaaaacaca gaggagtcat cggccagcgc ggcttctacg ttgatttgtt gatagttatc
     8761 gcctaggcca atccacggtt cgccagccgt aaacccggca ttatcgccgt tgctccattg
     8821 catgggcgtg cggctgttgt cacgggattt actggcgagg attgccaata actcgtcggc
     8881 atcacgacca tcgttgcgca gctcggcaaa catattgagg ctctccacgt cgcgatagtc
     8941 agtaatgcgc gtgaaatgcg ggttggtcat gccaatctct tcgccctggt agatatacgg
     9001 cgttccctgc atgccatgca gcaccatcgc cagcattttt gccgcaggca cgcggtattc
     9061 accttcatca ccaaagcgag aaacaatgcg cggctgatcg tggttacacc agaacaaggc
     9121 attccatgcc ccgttgtgca ttccttgttg ccagtggcgg aacaatgttt tcaacgccac
     9181 aaagtcaggt ttagccagcg tccatttttc accaccggga taatcgacct tcaggtgatg
     9241 aaaattaaag gtcatcgaca attcactgcc tgtcagtgcc gcgtatcgct ggcaatgctc
     9301 aaggctggtg gaggacattt cacctacggt cattaacccg cgtggcgtaa acacatcgcg
     9361 gttcatctcg tgcaaaaact cgtgtgctcg tggcccgtcg gtgtagaagc gacgcccgtc
     9421 gccatccagg tcttcaggga aacacgggtc tttggagatc agattcacca catccaggcg
     9481 caacccgtcg accccacgat cggcccagaa ttcacagact tttttcagct ctgcgcgtac
     9541 cgctggattc tcccagttga gatccgcctg ttctggtgca aagagatgca aataatactg
     9601 ttcgctttcc gcatgccagc gccacgcact accgccaaat tttgaacgcc agttgttcgg
     9661 tggcgtttct ggttcgccat cgcgccagat ataaaactgg cggtaagggc tttctttgtt
     9721 cagcgcctcg cgaaaccagg catgttgggt agaggtatgg ttaaacacca tatcgagaat
     9781 gatacgaatc ccgcgcgatt ttgcctgcgt caccagttcg tcaaaatcgt ccagcgtgcc
     9841 gtaggtggga tcaatcgccg tatagttcgc tacatcgtaa ccgttatcga cctggggaga
     9901 gacataaaag ggggttagcc agatggcatc aacgcccagt ttatgcagat agtccaggcg
     9961 ttggataacg ccacgtaaat cgccggtgcc gctacccgtg gtgtcctgaa aactctttgg
    10021 ataaatctgg tagataacgc cgttttgcca ccagtgggga agattagtca ttacgttatt
    10081 cctgcaaatg cgagggggcg caattgcgcc ccgaagaaaa ttaaacaatg tccagcgtgc
    10141 ccaggcggta tttccgctga tagataaacg aggtgagtac aatcgggatg atgatggcga
    10201 tagccattgc cagcgcaaat acctgccagt agctcggttg aatcgagaga attcccggca
    10261 ggccccctac gccgatacca ttcgccataa cgccgttcag gccgcatagc aatcctgcca
    10321 gaccagaacc aatcatcgcg cacagcatcg ggaagcgata tttcaggttg atgccgtaca
    10381 ttgcaggctc agtgacccca agccaggcgg agatagcggc aggcacggag atctcgcgtt
    10441 cattgtgctt gcggctggaa atgataatgc ctatcacggc ggagccctga gcgatattcg
    10501 acagcgcaat cagcggccac actggcgtgc cacccatgct ttgaatcatc tgcaaatcaa
    10561 tagcaagggt ggtctggtgt acgccggtga tcaccagcgg cgcgtacagg aagccaaaca
    10621 atgccgcacc aatcggagca aagctgccgg tcatcaggtg acgtaccgca aaggcaacgc
    10681 catcgccaat catgcgacca aacggaccaa tcagcgcatg ggcgaggaac accgcgagga
    10741 tcagcgaaca gacgggcacc accaccagat agaggtaatc cggtacgatg cgtttaaggc
    10801 gagtttcaat aacgcccagc gccagcccgg ctaacagtgc cgggatcacc tgcgcctgat
    10861 agcccacttt ggcgatgctg aacatgccaa agtcccacac ttccggcagc tgctgcccga
    10921 gcagataagc gttcatcagc tggggggaaa ccagtgtcac gccaagcacg ataccaagga
    10981 tcggcgtgcc gcccattttt ttcaccgctg accagcaaat cccgaccggc aggtagaaga
    11041 aaatcgcttc accgatcaac cacagaaaat cgtagatcgt ttgcagggaa gggtacattt
    11101 gcgccagcgt ctgaccgttg ctcatgggca aatcgccgat cacattgcga aaaccgagga
    11161 tcaaaccgcc gctaatcaac gcgggcagca acgggaagaa gatctccgcg aaatgagaga
    11221 tcaactgctc atgccatttc atattttgcc gggcggcttt ttttacctgc tctttatcaa
    11281 cctgcgcctg tccggttgac gctattagtg cttgatagta atcacccacg ttggtgccaa
    11341 tcaccacctg aaattgcccg gcattggtga aacagccttt caccatgggg agttgctcaa
    11401 tttctttcgg tctggcattg gccggttggt tgaggacaaa gcgcaggcga gtaatacagt
    11461 ggctcaccgt cgcaatattg ccgcgcccgc cgaccagttc aatcaaccga tcgatatccg
    11521 tttggtttat tttgctcatc ataaagcccc atggcagatg acatttttgg ttggctgcag
    11581 aatatattgc gcggaaaaat ttaaaaacgg gaacgttccc gaaacgcagc gaagatcaca
    11641 atttatcgtt caggaaacga tcaggacagg gtggcgggga tgatgatttg ttgcggttcg
    11701 ctgcgcccgg ttacctgtgc gatcaattgg caggccgcct ggcgtccagc ttcggcgtag
    11761 ccaggatcta cggtgacgat ctccggatga aggaatttca ttaacggcgt attacctacg
    11821 ctcgccagtt gcaaggtgtc gatgcgttgc tcttgcaggt atttacttgc gccaagagcc
    11881 agcgtgtcgg ttgcgcacag taacgcggta gtttcaggcg taatcacttt tgctacgttt
    11941 tcatagcctt gcttcatagc aagccctggc agagcggcaa cgggatgcag tttatgcgct
    12001 ttgcagaacg ccaggtaggc ttcgtgacgt cgcttcccgg tcgtcacgtc actatgcggc
    12061 acgccgagat aactgatatt gcgatgcccc tggtcataga gccgttgcat cagaatcttg
    12121 attgcccctt cgtcgtcata acagaccgaa gcaaagcctt ttgcgtcacg cgccagcaga
    12181 accagcgatg actgccagtg ggctaacatt tcttcggtta tgccggtaaa accgaacagc
    12241 actacgccgt cgatattacg ccgtttcagc acccccaaat gttcggcaac taattgtggg
    12301 gaaaactgat tttccatcat gattggatcg taaccttgtt catagaacgc tggcagcatg
    12361 gtttgaacgg cgagattttc tgacaacgaa tccagacggg taacaatgat ggcgaccact
    12421 ttatcgcttt gcccacgcat agcgcgcgca gagcgggaag gggaaaatcc atgctgattc
    12481 atcactgctt caacacgctc gcgcgtgcgc tgactcacgc cgctttcgtt attcagcacc
    12541 cgggaaaccg tagatttccc cacgccgctt aagcgcgcga tgtctttgat ggtcagccga
    12601 ttttgcatcc tgttgtccca taacgtgttg tttaattatt tgagcctaac tttacccgtg
    12661 cattcagcaa tgggtaaagt ctggtttatc gttggtttag ttgccagcag gtattatatc
    12721 gccataaatg ctacgaatat tattggattc tccttattat ttgtggcgct tttttcactt
    12781 accggaggtt atatggaacc tgatcccacg cctctccctc gacggagatt aaaacttttc
    12841 cggtaagccc gtcttttcac ggcgttaccg gatgcgtaag gccgtgacgt tttaatgtcc
    12901 ctgctcagct ttattacctt caggtaaggc ttcgccacgc ctgaagacat ttctgtactg
    12961 tttcagacag tgcggaggga ctccttatgt ttaaagaaat ttttacccgg ttcattcgcc
    13021 atttaccttc ccgtctggtt catcgcgatc cattgcctgg cgcgcagcag acagtgaata
    13081 cggcggtccc gccgtcctta agtgcgcatt gcctgaaaat ggcggtgatg cccgaagaag
    13141 aattgtggaa aacgttcgac acccatccgg aaggattaaa tcaggcggaa gtggaatctg
    13201 cccgcgaaca acatggtgat aataaattac ccgcacaaca accgtcgccg tggtgggtac
    13261 atttatgggt ctgctatcgc aaccccttta atattttgct caccattctt ggtgctattt
    13321 cttacgccac ggaagattta tttgccgcag gtgttatcgc gctaatggtc gctatttcta
    13381 cgttgctgaa ctttattcag gaagctcgtt ccaccaaagc ggcagatgcc ctgaaagcga
    13441 tggtcagtaa taccgcgacg gtactgcgag taattaacga caaaggcgaa aatggctggc
    13501 tggagatccc gatagaccag ctggtgcccg gcgatattat aaaactggcg gcgggggata
    13561 tgatcccggc agatttacgt atcttgcagg cgcgggatct gttcgtcgct caggcgtcgt
    13621 taaccggtga gtctctgccc gtggaaaaag ccgctactac tcgccagccg gagcacagca
    13681 atccgctgga gtgcgacacc ctgtgtttta tgggcaccac cgtggtgagc ggcacggcac
    13741 aagcaatggt gattgctaca ggagccaaca cctggtttgg tcaactggcg gggcgtgtta
    13801 gtgagcagga aagcgagccg aatgcctttc agcaagggat cagccgcgtt agtatgctgc
    13861 tgattcgctt tatgctggtg atggctccgg tggtgttgct tatcaacggt tacaccaaag
    13921 gcgactggtg ggaagcggca ctgtttgcgc tttcggtcgc ggtaggctta acgccggaaa
    13981 tgttgccgat gattgttacc tcgacgctgg cgcgcggcgc agtaaagctg tcgaaacaga
    14041 aagtgatcgt caaacatctg gatgccattc agaactttgg cgcaatggat attctgtgca
    14101 ctgataaaac cggcaccctg acgcaggaca aaattgtgct ggagaatcat accgatatct
    14161 ccggtaaaac cagcgaacgc gtgctgcata gcgcgtggtt gaacagtcat taccagaccg
    14221 gacttaaaaa tctgcttgat accgcggtgc tcgaaggtac ggatgaagag tcagcgcgtt
    14281 cgctggccag ccgttggcaa aaaattgatg agattccgtt tgatttcgag cgtcgtcgga
    14341 tgtcggtggt agtggcagaa aataccgaac accatcagct ggtttgcaaa ggtgcattgc
    14401 aggaaatcct caatgtgtgt tcgcaggtgc gtcacaatgg cgagattgta ccgctcgatg
    14461 acaccatgct gcgtaagatt aagcgggtta ctgatacgct gaatcgtcag gggctgcgcg
    14521 tggttgcggt ggcgacgaaa tacctgccag cgcgtgaagg agattaccag cgggcggatg
    14581 aatccgacct gatcctcgaa ggatatattg ctttccttga tccgccaaaa gagacaaccg
    14641 ctccggcact gaaggcatta aaagcgagtg gaattaccgt aaaaatcctt actggcgaca
    14701 gtgagttagt tgcagcgaaa gtgtgccatg aagtggggct ggatgcggga gaggtggtca
    14761 ttggtagtga tattgaaacg ctatctgacg atgaactggc aaatctcgca cagcgcacca
    14821 cgctgtttgc ccgcctgacg ccgatgcata aagaacgcat tgtgacctta ctgaagcgcg
    14881 aagggcatgt ggttggcttt atgggcgatg gtattaatga tgcgcccgcc ttacgcgctg
    14941 cggatatcgg catttctgtg gacggcgcgg tagatattgc ccgtgaagcg gctgatatca
    15001 tcctgctgga aaaaagcctg atggtgctgg aagagggggt tattgaggga cgtcgcactt
    15061 tcgccaacat gctgaaatac atcaaaatga cggcgagttc taacttcggt aatgtgttca
    15121 gcgtgctggt ggcgagtgct ttcttgccct tcctgccgat gttgccgtta cacttgctta
    15181 ttcagaacct gctgtacgat gtgtcacagg tggcgatccc gtttgataac gtcgacgacg
    15241 agcaaattca aaagccgcag cgttggaatc cggcggatct ggggcgcttt atggtcttct
    15301 tcggaccgat cagctcgatt ttcgatattc tgacgttttg cttgatgtgg tgggtattcc
    15361 atgccaacac gccggaaacg caaacgctgt tccagtcggg ctggtttgtg gtgggattac
    15421 tgtcgcaaac gctgattgtg catatgatcc gtacccgccg tgtgccgttt attcagagct
    15481 gtgcatcgtg gccgttaatg atcatgaccg tgatcgtgat gattgtcggg atcgcattgc
    15541 cgttttcacc gctggccagt tatctgcagc tgcaggcgct gccgttaagc tatttcccgt
    15601 ggctggttgc gattctggca gggtatatga cgttaaccca gttggtgaaa gggttctata
    15661 gccgtcgtta cggctggcag taaagaataa actgggcatg gtggcccgga ttcagattgc
    15721 agacaaagtg agctttgttc atgccggatg cggcgtaaac gcccaatccg gcctacaaat
    15781 tcaataaatt gcaggaatca cgtaggcctg ataagcgtag cgcatcgggc gattttgctt
    15841 ttgtcatcag acttaatccg ggcatgatag cccggatttc catcaagatt agcgacgaac
    15901 agcgattgct tcgatctcaa tcttcacgtc tttcggcaga cgggcaactt caacgcaaga
    15961 acgtgccggg aaagtggcgt tgtgttcggt gaagaaggct tcgtaagtgg cgtttacggt
    16021 tgcgaagtcg ttcagatctt tcacgaacac agtggtttta acgatgtcgc ccactttcag
    16081 gccagcggct tcgacgattg cttttacgtt atccagcgac tgacgtgcct gtgcagcgac
    16141 gtctgccggt acttcaccag ttttcggatt taccgggatc tgaccggagg tgatgatcat
    16201 attgcccaga tcaacgccct gtacgtaagg accgatagct gccggtgcat tttccgtcgc
    16261 gatagttttg ctcatgattt ctcctttatt acagcggtaa aaagtctggc tcattatagg
    16321 gagccagact tttattacca accgcaatta attggccagc accacattat gggaaaactc
    16381 tttttcacag tatttgcatt taagcgcgat gtcattggcg cgttttcgca cggcaaagct
    16441 ggatgaaacc ggttcggcat ggctgataca gttgctgttc gggcagacca gcacattgtc
    16501 gatgcgctcc ggcagacttg ggcgcgattt acccaccact tcatagttgt cgatacggtt
    16561 aaccgtggct tgcggtgcat acaatgccag ttggtctact tgatcttcac tcaaaaaggt
    16621 attttcgatt ttgatcagat ctttgcggcc catctcgcca gaaggcaaat tcagaccaat
    16681 ggtgatgcgc tgatctgttt cggtcaactt gaacagactc aacagcttaa aaccgatctg
    16741 ggcggggata tggtcaatta ccgtgccgcg tttaatagct tcaacctgca atttattatc
    16801 gtgtgtcatc tctatttccc cttacagtac cagatcgcga ttcagaacca gtgccagtaa
    16861 cgcctggcga gcgaaaattc cgttgcctgc ttgctggaag taccaggcgt gaggcgtttt
    16921 atccacatcc gtcgcaatct catcaacacg cggcagcgga tgcagcactt tcatattggc
    16981 tttggcgttg tggagatcgc tggcgcgaag aacaaactgc gctttcacgt tggcgtactc
    17041 ggacgggtcc agacgctctt tttgcacgcg ggtcatgtac aggatgtcta cttccgccat
    17101 cacttcttca atagagctgt gcagactcca ggcgatccct ttttcatcga gcatatccag
    17161 aatgtattgc ggcattgcca gcgcatccgg cgcgataaag taaaaacggt tgccgtcgaa
    17221 cttcgctagc gcctgggtca gggagtgaac ggtgcggcca tatttcaggt caccaaccat
    17281 tgcgacgtgg agattgtcca gacgcccctg ggtttcctga atggtgaata agtccagcaa
    17341 ggtttgcgtc ggatgttggt tggagccatc accggcattc agtaccggta cattgccaga
    17401 aaattcggtg gccaggcgcg ccgcaccttc ctgcggatga cgcatcacta tcgcatcgac
    17461 gtaagtgctg ataaccgaaa tggtatcggc cagcgtttcg ccttttttac ccagtgatgt
    17521 attggcgctg tcggagaagc ccaccacgct ggcacccagg cggtgcatgg aagtttcgaa
    17581 agagaggcgg gtacgggtag aggcttcgaa gaaacagctg gcaatgactt tgtgcttcaa
    17641 cagctctggt tgcgggtttg ctttcagttt cgccgctgtc gccagcacca gattaaggtc
    17701 atcgcgacta aggtcgttta tggaaatgat atgtttctga tatagcggat tagccatctt
    17761 ttatctcctg acgcctaggc aaaaaaaagc ccctcgattg aggggctggg aatgggtgat
    17821 caacgggaag aaaaacggca ggccagcgtc ttttttcaga cgcggtaaga caaaatgtcg
    17881 aacacactga accatacatc ctcccggcaa attgtccggc attatactca tagccagaag
    17941 cggtgcaagc atttgatgca atattttgtc agcgcaaacg gtttatttga attaaaagtc
    18001 aaaatatatg catttttatt ttctatgatt ctggagggga agtgtcgttc ggtatcaata
    18061 attgcagaca tttcctgctt ttttcttttt cattttcacg caatcagaaa tgcattttat
    18121 tccattcatc cgacttatag ggcggagggt gaaaacgaac ggctaacact attgcttata
    18181 gctcagggat acgcgctatc actttaattt caaaatcaaa gcctgccagc caggtaacac
    18241 ccaccgccgt ccagtttgga taaggtgggg cgctaaatat ttcatttttc accgtcatga
    18301 tgtcttcaaa ttgtttttcc ggatcggtat gaaagctcgt aacatcaatg atatcgtcaa
    18361 aagtgcatcc cgcagctgcc agggtcgcat gcaaattatc aaaggccagt ctgacttgtt
    18421 gctgaaaatc gggttctggt gttccgtcct ctcgacttcc tacttgcccg gaaacaaaca
    18481 gcaaatcgcc ggaacgaata gccgcagaat aacgatgctc agcatacagt gaatgtcggc
    18541 cagcagggaa aacagcggtt ctttctacca tttggttatc ctcaagattt acgacatgaa
    18601 cagaagattt atatttaccg ggagccgctt ttagggggac gacgttagta aacaaaaccc
    18661 agacatcatg gataatggct gggcttaatt gagcgtagtc ggttatgcgc caaacgcgcc
    18721 atcaatggta tgcatcgcgc cggtaacaaa actggcttct ggccctgcta accatgcgac
    18781 cataccagcg acctcttccg gttgcccatg tcttttgatg gccataaaac tatgcaacat
    18841 atcgcgtatt ggcccgttgg cgggattaac gtcggtatca attggtcctg gctggacgac
    18901 gttgatggtg atccctcgcg gtccaaaatc acgggccagc ccgcgcgcca tgccttgcag
    18961 ggcagattta ctggcggcat aagcagccat gcctgcaaca ggcatacgat cgccattcac
    19021 ggagccgatg attaagatgc gcccgccttc gggcatctgc cgggcggctt caacagaggc
    19081 atgataagga gcatgaatat tgattttgaa aaggcgatca atatcgtcgg catttaattc
    19141 cagggcatcg ccaaagacgc caatacctgc atttaccacc aggatatcca atgtgccgct
    19201 cttacgaacg acatcaataa cagcgtctct gtcagcacta tctgtgaata ctgctgtcgc
    19261 tccagtctct tgtgccagac gttcagcggc atctttcgac cccgcatagg tgaatcgtac
    19321 attggcccca tcggtgacga aacgacgtac gatagcggca ccgattccac gactgccacc
    19381 gaggatgaga actgtcttac cggtaaaagc gcccataagg actccttgat ttattatgta
    19441 acatgcatta caaaactgtt ttaactttct gtcaacaagt tttgtaatgg tcactaaaaa
    19501 acaatctcgc gttccaggtc gtcccagacg tttcgctcct gagcaggcag tctctgcggc
    19561 aaaagtgctt tttcaccaaa aaggtttcga tgcagtaagt gtctctgaag ttactgatta
    19621 tcttggtatt aaccccccga gcctctacgc ggcttttggc agtaaggctg ggttatttag
    19681 ccgtgtactc aatgaatatg tcggtacgga agctattccg cttgccgata ttcttcgtga
    19741 tgatcgtccg gtaggcgagt gcctggttga ggtattaaaa gaagcggcgc gcagatatag
    19801 ccaaaacggc ggctgcgctg gctgtatggt tcttgaaggt attcatagtc atgatccaca
    19861 agcgcgtgat attgccattc aatattatca cgccgcagaa acgaccattt atgactatat
    19921 cgccaggcgg catccacaaa gcgcacaatg tgtgactgat tttatgagta ccgtgatgtc
    19981 agggctttct gcgaaggcac gggaggggca ctccatcgaa caactctgtg caacagctgc
    20041 cctggcgggg gaagcgataa aaactcttct caaggagtga tgctggcctt gatccgaaag
    20101 gcgggaacgc gcctgccgat aagttgtgat aagacaataa ttcacgcgtt aaggctggcg
    20161 gaattgatca tcttttcgta taacgataga aatgaaacat tgttttaatt aaggagtgga
    20221 aaagatgatc atcggaaata ttcataatct tcagccgtgg ctaccccagg agttacgcca
    20281 ggcgattgag catatcaaag cacacgttac ggcagaaacg ccaaagggca agcacgatat
    20341 cgaaggcaat cgtctgtttt atcttatctc ggaagatatg accgagccgt acgaagctcg
    20401 ccgtgcggag taccatgccc gctatcttga cattcagatt gtgttaaaag gtcaggaagg
    20461 catgaccttc agcacgcaac ctgcaggcgc gccggatacc gactggttag ctgataaaga
    20521 catcgcattt ttgccggaag gcgttgatga gaaaacagtt atcctaaatg aaggtgattt
    20581 tgttgtgttt tatccggggg aagtgcataa accgctgtgc gcagtgggcg cgccagccca
    20641 ggttcgcaaa gcagtagtga agatgctgat ggcgtgatga cttttcgccg taaataactc
    20701 caggtttacg gcgagtttgt gaaaagagcg ttttttgata tttttttgtg agtaaaattt
    20761 gtaatgctta gacgttctta ttaactcaag gagttcgtca tgagcaaaat atcagatttg
    20821 aattattctc aacacattac attagccgac aattttaaac aaaaaaatga agccttagat
    20881 acctggtatg tgggaatgaa tgattttgcc cgaattgccg gggggcagaa tagcagaagc
    20941 aatattcttt ctcctagagc atttttggag tttttggcaa agatatttac cctgggttat
    21001 gtggatttta gcaaacgctc caacgaagcg ggtagaaata tgatggctca tattgagttc
    21061 tcatcttatt ctaaagtact gatggcaatg aaaaaatgaa gttttacatg aataatcctg
    21121 aaggggagcg agcggattta tctaaggtga agacagaaat tacacttgcc tcagcttcta
    21181 ctaaagggat tcgtgaagga catacagtca ttatatttaa acaatctgat ggttcgacta
    21241 atcgttatga agggaagtct tttgaaagaa aagatgatag ttcattacac ctgattacta
    21301 acaaggttct ggcgtgttac caacgcgaag ctaacaagaa aatagcgcgt ctattaaata
    21361 atcatcagaa gttaaataat ctacagaagt taaataatat acaggagtta aatgattcgc
    21421 aggagttaaa taattcacag aagttaaata attcgcagga gttaaatgat tctcaggagt
    21481 taaataactc gcaggtgagt tgtaaaggtt cagttgattc tacgattacg gatttattag
    21541 aaaaagcatt gaataaagcc ttattagcaa taaggaacga acatctgcta ttaatgcctc
    21601 atgtatgtga tgaatagatt tcatacttac tgggcgaaaa tggtatactt gaagaaatag
    21661 ataagctcta cgaattaaat gatcacggaa ttgataatga caaagaaggt aacaatgaaa
    21721 ttaatgacat catgattaac ctgtctcata ttcttattga ttccttagat gatgcaaagg
    21781 ttaaccttac accggtcatc cattcgatgt tggtgacttt tttagaattg ccatataata
    21841 atgatgtaaa aatactggag tggtgtttta ataaaagcat gcaatatttt gatgattctg
    21901 caaagataga gcatgcatgc tccgtaataa atcatattaa ttttctctgc gatcagtcta
    21961 aagtagctga gacattattt ttcaatctcg ataaagaacc ctataaaaat agccctgaat
    22021 tacaggagtt gatttggaaa aagttggttg tatatgtcaa tgattttaac ttaagcaatc
    22081 gagaaaaaac atatttaata caaaggatat ttaataatgt tgagtcgcta tttaataaag
    22141 tacctgtcag cattttagtt aatgatattc ttctgaatga tttttttatg aaaaacactg
    22201 agatgattaa ctggtacttt cctcggttac ttaagagtta tgagggcgaa aaaaattatt
    22261 ttgataattt aaaatatgat ataaatgatg agaaatctaa taaagaaatt ttgaagaatc
    22321 aaccagataa tgttatcaaa gaaaaactga ataatgaatt caaacttaga tttagaatga
    22381 tgcaaactat ctttaaatcg aaggttaatg tatcgccata tattgaccaa cagcgtttaa
    22441 atacactaaa tcctccggaa aatttacgta tggcaataga aaaatttggc tggaagaaaa
    22501 aaactatcac tgcataaaat aatttgatgc cgggatgttt ttgtatttcc cggcatcttt
    22561 atagagatat taattattta atttactgag cgtcgcgacc atcaccgctt tgatggtatg
    22621 catgcggttt tccgcctgat caaatacaat gctggcggca gattcgaaga cctcatcagt
    22681 cacttccatt ccgccatgta ggccaaactc ttccgccatt tttttgccaa gcgtcgtttg
    22741 gtcgtcatga aacgcgggca ggcagtggag gaatttgacc tccgggttac cggtcaactg
    22801 catcatcttg ctgttcacct gataatcacg cagtaatgca atgcgttcag cccatttttc
    22861 ttttgcttca cccatcgaca cccatacatc ggtatagata aagtcagcac cttcaactcc
    22921 tttagctaca tcttccgtca gcgtaatatt cccgccattt tgttgtgcca gggcgcggca
    22981 ttccgtaacc agcgcagctt ctggccagca tgcctgtggc gcgaccagac gcaaatccag
    23041 accggtaagc gccgcagctt cgagcatcga attgcccatg ttgttacgtg cgtcacctgc
    23101 atagaccagc gtcatttcgt tgaatgcttt accgggtaaa tgctcctgca tggtgaggag
    23161 atccgccagc aactgcgtgg ggtgaaactc attggtcagg ccgttccata ccggcacgcc
    23221 agcgtattcc gccagtgttt cgacaatctc ctgaccatag ccgcgatact gaataccgtc
    23281 atacatgcga ccaagcacgc gggcagtgtc tttaatcgac tctttatgac caatctgact
    23341 accgcttggg ccaagataag taacgcgagc accctggtca tatgcggcaa cttcgaaaga
    23401 gcatcgggta cgagtcgagt ctttttcgaa gatgagcgcg atgtttttac cagtgagttt
    23461 ggcttcttct ttaccgcttt tcttatcagc tttcagcttc gcggctaact gcagcaggct
    23521 gttgagttca gctggcgtga aatcgagtaa tttcaggaaa tgcttatgat aaaacccgga
    23581 catagatccc tcctgtggct aacgcctcaa tgaattaaaa ttcaatttat atgtatgatt
    23641 attcatttgc aagtctaaag cataaatctt tgtcacaaag gtggaggcaa tgtcagtggt
    23701 gtgtgacaat aagagtatcg gcaggacatt aagaggaatg agccatggca aacccggaac
    23761 aactggaaga acagcgtgaa gaaacacgtt tgattattga agaattactg gaagatggca
    23821 gcgatccgga cgcgctgtac accatcgaac accatctttc cgcagacgat ctggaaactc
    23881 tggaaaaagc ggcagttgaa gcgtttaaac tcggttacga agtgaccgat ccagaagagc
    23941 tggaagttga agatggtgat atcgtgattt gctgcgacat cctcagcgag tgcgcgttga
    24001 atgccgatct gatcgatgcc caggttgaac aactgatgac gctggcggag aaatttgacg
    24061 ttgagtacga cggttggggc acttactttg aagatcctaa cggcgaagat ggcgacgatg
    24121 aagattttgt cgatgaagac gatgacgggg ttcgccacta attaattgat taaaatgccc
    24181 gatagcacat actagcgggc atttttttag agttcgcgta gcatccgcac ttcacaatcg
    24241 acatggcccg tgcagccaag cgcatagtcg atatgctcaa agcccaaatg ctcataaagc
    24301 gcaatggctt cctttaaaaa agcggtcgtt tccagatagc agcgtttgaa gcccatctct
    24361 cgtgcctgct ccatcgccat taaggccagt tttttcgcta gccctttacc gcgaatagcg
    24421 ggaagaaagt acattttctg cagttcgcaa atgtccgctt cactacctgt taatggcgca
    24481 atcccgccgc cgccgaccac ttcgccattg tattcaacca cccaataagc atggccaggc
    24541 tggctgtaca cttgatacag ttcgtcgaga ttcggatcgg caacggtgta gcctttatcg
    24601 gcggtaagac catactctgc gtaaacctga cggatgacgc tggcgatagc aggattatcc
    24661 tgcagcgtca ggcgacgcat tacaggtgat tgcggcgcaa tgttattcat agtttaaccc
    24721 aataaaaata aagtaattat acatttgttt aataccactc tcttcttacg tgcgcaagcg
    24781 tcttataaag caataaagtt tttgccagcc tattactgat aagagtattg taacgtggca
    24841 gaacatttta ctgtctgtat ctataaatta tatcaatgga ttttttaatt caggggattt
    24901 ttatggctca agttattaat gaaatggatg ttccgtccca ttcttttgtt tttcatggta
    24961 caggtgagag atattttctt atttgtgtgg tgaatgtgtt gttaacgatt ataacgctag
    25021 gtatctattt accatgggca ttaatgaaat gtaagcgtta tctttatgct aatatggaag
    25081 ttaacggaca acgattttct tatggaatta ccggtgggaa tgtttttgtt agttgtcttg
    25141 tttttgtttt tttctatttc gcaatcttaa tgacagtgtc agcagatatg ccgcttgttg
    25201 gctgtgtttt gactttgtta ctgttggttt tgcttatatt tatggcagca aaaggactgc
    25261 gttatcaggc cttaatgacc agtctcaacg gcgtaagatt tagttttaat tgctctttga
    25321 aagggttctg gtgggtaacc tttttcttgc cgattttaat ggccattggg atggggactg
    25381 ttttctttat ctcgacaaag atgctacatg ccaatagttc aagtagtgtt attatatctg
    25441 tggttctgat ggcaatagtt ggtattgttt ccattggtat ttttaatggt actttatata
    25501 gcctggtaat gagttttctc tggagcaata ccagtttcgg tatacatcgt ttcaaggtga
    25561 aattagatac tgcgtattgt ataaaatatg ccatcctcgc atttttagct ttattacctt
    25621 ttctcgctgt tgctggttat attatcttcg atcaaatatt aaatgagtat gatagttctg
    25681 ggtatgcaaa tgatgatatt gagaatttac agcaatttat ggaaatgaaa cgtaaaatga
    25741 taatcgcgca gttaatttat tattttggga ttgctgttag caccagttat ttaacggtgt
    25801 cgttgcgaaa tcattttatg agcaacctgt cattgaatga tgggcgtatt cgttttcgct
    25861 caactttaac gtaccacggt atgctttatc gcatgtgtgc gttggtggtg atatccggga
    25921 ttacgggtgg tctggcttac ccactgctga aaatatggat gattgactgg caggcaaaaa
    25981 atacgtattt gctgggcgat ttggatgacc ttcctttaat caataaagaa gaacaaccag
    26041 ataaaggctt cttagccagt atttctcggg gagttatgcc ttctttacca tttctgtaag
    26101 agataaaaaa ggccggagca tgctccggcc ttcgttttca tcactgtgtt gattacagtg
    26161 cggcgataac agcctgctgt tcaatcagct tggctttcgc ttctgcataa ccttccagct
    26221 tctcacgctc tttcgcgata accgcttccg gtgcgcgggc gacaaagcct tcatttgcca
    26281 gcttgttctc gatgcggctg atttcacctt cgatcttcgc cacttctttc gccaggcgcg
    26341 ccagctcatc ttctttgttg atgaggccag ccatcgggat cagcagctct gcgccgtcga
    26401 tgatcttggt aacggaaacc ggacctttgt catcggcagg cagcacggtg atgctttcca
    26461 gacgcgccag cgtttgcagg aagccacggt tttcatttac gcgacgttct gcatccgcgc
    26521 tgcaaccacg cagcagcagc tccagcggtt tgcccggcgc gatgttcatt tctgcacgga
    26581 tgttacgtac agcaacgatc gcctgtttca gccactcggt gtcggccagt gcggcttcat
    26641 caacctgaga tgcatcatac tgcgggaacg gctgcagcat gatggtgtcg gcagtgatac
    26701 cgcaaagtac tttcacacgc tgccagatgg tttcggtgat gaacggaatg atcggatgcg
    26761 cgaggcgcag cagaccttcc agtacagtca ccagcgtatg gcgagtaccg cgcagttcgg
    26821 cttctgttcc accgttcatt accggcttgg tcagctccag ataccagtcg cagaactggt
    26881 tccaggtgaa ctcatacaaa atgcctgcgg cgatatcgaa gcggaagctg tccagcgctt
    26941 cgcggtacgc tttgatagtc tggttgaact ccgccagaat ccagcggtcc gccagcgata
    27001 gcgtcatttc gccgccgttg aagccgcaat cctgaccttc tgtgttcatc agcacaaagc
    27061 ggctggcgtt ccacagcttg ttacagaagt tacggtagcc ttccagacgc ttcatatccc
    27121 agttgatgtc acgaccggta gacgccagcg ccgccagggt gaagcgcagg gcatcggtgc
    27181 cgtgcggctc aatgccgttc gggaactgtt tctcggtgcg cttacggatt ttgtccgcca
    27241 gctgtggctg catcatattg ccggtacgtt tttccagcag ttctggcagc gaaataccgt
    27301 caaccatatc cagcgggtcg ataacgttac ccttggattt ggacatcttc tgaccttcgt
    27361 cgtcacgaat cagaccggtc atgtaaacag tgtggaacgg cacctgcggt ttgccatttt
    27421 catctttgat gaagtgcatg gtcatcatga tcatgcgggc aatccagaag aagatgatgt
    27481 cgaaaccaga taccatcacg ctggttgggt ggaactgacg cagggcgtcg gtattttccg
    27541 gccagccaag ggtagagaag gtccacagtg cagaagagaa ccaggtgtcg agaacgtctt
    27601 cgtcctgacg cagggcaacg ttggcaccga ggttattttc cttgcgcact tcttcttcgt
    27661 tgcggccaac ataaacatta cccgcttcgt cataccatgc cgggatacgg tgaccccacc
    27721 acagctgacg agagatacac cagtcctgaa tatcgcgcat ccaggagaag tacatgtttt
    27781 cgtactgctt cggtacgaac tgaatgtcgc cgttctcaac cgcttcaact gccggtttcg
    27841 ccagtacatc ggcacgcacg taccactggt cggtcagcat cggttcgata accacgccgc
    27901 cacggtcgcc gtaaggaacg gtcaggtcgt gcggtttaat ttcttccagc aggccgagtg
    27961 catcaaccgc agcaacgact gctttacgtg cagcaaaacg ctcgagtttc tggaactctg
    28021 ccgggatctc gctggaataa acgtcagatt cgttaccttt ggtatcgaac acctgggcgc
    28081 tttcacggat atcaccgtca aaggtcagga tgttgatcat cggcagggcg tgacgtttac
    28141 cgacttcata gtcgttaaag tcgtgcgccg gggtgatctt cacgcagccg gtgccttttt
    28201 ccatgtcggc gtgttcgtcg ccaacgatcg gaatacgacg gttaaccagc ggcagaatga
    28261 catatttgcc aatcagatct ttgtaacgcg gatcttccgg gttaacagct acaccggtat
    28321 cgcccagcag ggtttccggg cgagtggtcg caaccaccag ataatcttta ccgtctgcgg
    28381 ttttcgcgcc gtcagccagc ggatagcgga tgtgccacat cgaacctttc gattcgtggt
    28441 tttccacttc caggtcagaa atagcggtac gcagtttcgg gtcccagttt accaggcgct
    28501 tgccacggta aatcaggtct tctttataca gacgaacgaa aacttctttc accgcattgg
    28561 acaggccttc gtccatggtg aagcgttcac gctcccagtc gacggagttg ccgagacggc
    28621 gcatctgacg ggtaatggtg ccgccagatt ccgctttcca ttcccagatt ttgtcgatga
    28681 aagcttcgcg gccgtagtcg tgacgggttt taccttcttc tgcggcaatc ttgcgctcaa
    28741 cgaccatctg ggtagcgatc ccggcgtggt cagtaccgac ctgccacagg gtgtttttgc
    28801 cctgcatgcg ctgatagcgg atcatggtat ccatgatggt ttgctggaag gcgtgaccca
    28861 tatgcaaact gccggtgacg ttcggcggcg ggatcatgat gcagaaactt tcctggcttt
    28921 catcgccatt aggcttaaag tagccctgct tttcccagtg ctcgtaaagc ggctgttcga
    28981 tatcttgtgg gttgtatgtc ttttccatta tttccaggtt gccgtattca ggttgaaacc
    29041 agccacgcgg taggctttat agcgttcgcg cgccagttgt ttcagagaat cttcataagg
    29101 aacgaagtct accacttctg tgaaagcggt ggcaaaatct gcaaagcttg ttcgcagact
    29161 aatcaatata tcgcgcgggc tgctgctgcg tttttgcggc caggcgatct ccaccggcgc
    29221 accgccgcgc ggtccttctc ccgctaaatt atgcggaaca aagctttctg ccggacgcgc
    29281 ccacagggct tcatccagcc ggtaagcctg cttttcatct tcacaggcga tgagtacgcg
    29341 cttaccgctg cgccaacgtt ctgcggcaat ttcacacacc agttgctcaa cggcgcttaa
    29401 gccatcgacg gtggtgtcat tgtccagaag gtagaacgtt gcgtttttca tatatggggc
    29461 ttcttgtggt ggatttaaat attgctgcat tcctcaccct gaaagatgag caatgaattt
    29521 ctattgcgct cgatcggttc cctctccctt agggagaggg ttagggtgag ggtaaaacgc
    29581 aaacttactc ttcgccgtta aaccgagcgc ggtttaacag gaactgtgcc agcaacgcta
    29641 ccggacgacc ggttgcgcct tttgctttac cagaacgcca ggcggttcct gcaatatcca
    29701 ggtgcgccca gttatactta cgggtaaagc gtgacaggaa gcaacctgcg gtaatcgccc
    29761 caccaggacg accgccaatg ttcgccatat cggcaaaatt ggactccagc tgttcctgat
    29821 actcgtcacc cagcggtaag cgccatgcgc ggtcaccgga ttgttcagac gcggcaatca
    29881 gttcatgggc cagcggatta tggttcgcca tcagaccagt aatatgatga cccagcgcga
    29941 tcacgcaggc accggtcagc gtcgccacgt caatcactgc ttccggctca aaacgttcaa
    30001 cgtaagttaa cacgtcgcac agtaccaggc ggccttcggc atcggtgttc agcacttcaa
    30061 cggtttgacc ggacatggtg gttaacacat cgcccggacg ataggcacgc ccgccaggca
    30121 tgttttcaca gcctgccagc acgccaataa cgttaatcgg cagttgcagc tccgcgacca
    30181 tacgcatcac gccgtaaacc gccgccgcgc cgcacatatc gtacttcatc tcatccatgc
    30241 cttctgaagg cttgatggag ataccgccgg agtcgaaggt taaaccttta cccaccagca
    30301 cgattgggcg tgcatcttcc gacgcgttgc ctttgtactc aatcaccgac atcagcgatt
    30361 cgttttgtga accctgaccg accgccagat aagagtgcat tcccagctct ttcatctgct
    30421 gttcgccgat aacgcgggtg atgacattct tgctgtagct gtcagccagc tggcgcgcct
    30481 gagaggcgag ataagcggcg ttacagatat tcggcggcat attaccgaga tctttcgctg
    30541 cttttatccc ggcggcaatc gccagaccgt gctggatcgc gcgctcaccg ctggtcagtt
    30601 cacggcgggt cggcacgttg aacaccattt tacgcagcgg acgacgcggt tcgctcttat
    30661 tcgttttcaa ctgatcgaaa ctgtagagcg tctcttttgc tgtctcgaca gcctgacgca
    30721 ctttccagta gttgttacgg cctttaacgt gcagttcagt cagaaagcag accgcttcca
    30781 tcgagccagt atcattcagc gtattaatgg ttttctgaat aacctgcttg tactgacgct
    30841 catccagctc acgttctttg ccgcaaccaa taaggagaat tcgctcggaa agtacattcg
    30901 gaacatggtg cagcaacaat gtctgccccg gttttccttc cagttcgccc cggcgtagca
    30961 gggcgctgat gtacccatcg ctgattttat cgagctgttc tgcaatcgga gaaaggcgac
    31021 gtggttcgaa gacgcccacg acgatgcagg cactccgctg tttctccggg ctaccgcttt
    31081 ttacactaaa ctccatgcac tacgctcctg aatcttaaag acaacggcgg tggctacaga
    31141 tagaattgca agctttcgta actcatgtcc gctgttgcga tgacttcgtg ttaatcttaa
    31201 cgttattacg gcattggcac gtcagaacaa aatctgagag gtgaatccgt tgagtataat
    31261 tatcttagcg acgatttcga cgactcaaga gaataaatga cgtttaagcc atgaaacaag
    31321 ctaaaatcct gcaaaagacg agtttttacg ggcgtattta aagtgataat cataagatat
    31381 ctggtgcggg agacgctcaa aagccagctg gcgatactct tcatcttgct tttgatcttc
    31441 ttctgtcaaa agttagtgag gatcctcggc gcagcggttg acggcgatat tccggcgaat
    31501 ctggtgctct cccttctcgg gttgggcgtg ccggaaatgg cgcagcttat cctgccatta
    31561 agcctgttcc tcgggctgct gatgacgctg ggcaaactgt ataccgaaag tgaaattacg
    31621 gtaatgcatg cctgcggctt gagcaaagcg gttctggtga aagcggcaat gatccttgcg
    31681 gtattcacgg caatcgtcgc ggcggttaac gtgatgtggg cgggaccgtg gtcatcgcgt
    31741 catcaggatg aagtgttagc agaagcgaaa gcgaaccctg gcatggcggc gctggcgcaa
    31801 gggcaattcc agcaagcgac taatggcagc tcggtgctgt tcatcgaaag cgttgacggc
    31861 agcgatttca aagatgtgtt cctcgcacaa attcgaccaa aaggtaatgc acgtccttct
    31921 gtggtggtgg ccgattccgg acatttaacc cagctgcgcg acggctccca ggtcgtcact
    31981 ctcaaccagg gaacgcgctt cgaaggcact gcactgttac gtgatttccg cattacggat
    32041 ttccaggatt atcaggcgat cattggtcac caggcggtgg cgctcgaccc gaacgatacc
    32101 gaccagatgg acatgcgcac attgtggaac actgacaccg atcgtgctcg cgcagaactg
    32161 aactggcgta tcacgttggt attcaccgtg tttatgatgg cacttatggt tgtaccgttg
    32221 agcgtggtta acccgcgtca gggacgcgta ctgtcgatgc tgccagccat gctgctgtat
    32281 ctacttttct tcctgatcca gacctccctg aaatcgaacg gcggtaaagg taagctggac
    32341 ccgacactgt ggatgtggac cgttaacctg atttatctgg ctttggcgat tgttctcaac
    32401 ctttgggaca ccgtgccggt ccgccgcctg cgcgccagtt tttcgcgtaa aggagcggtg
    32461 tgatgcaacc ttttggcgta cttgaccgct atatcggtaa aactattttc accaccatca
    32521 tgatgacgct gttcatgctg gtgtcgctgt cgggcattat caaatttgtc gatcagctga
    32581 aaaaagccgg gcaggggagt tacgacgcgt taggcgcagg aatgtatacc ttgctgagcg
    32641 tgccgaaaga tgtgcagatc ttcttcccga tggcggctct tcttggggcg ttgcttggtc
    32701 ttgggatgct ggcgcagcgc agcgaactgg tggtgatgca ggcttctggt tttacccgta
    32761 tgcaggtagc gctgtcggtg atgaaaaccg ccattccact ggtcttgctg acgatggcga
    32821 ttggcgaatg ggtcgcgccg cagggcgaac agatggcgcg taactaccgt gcgcaggcga
    32881 tgtacggcgg ctcgttgctc tctacccagc aaggtttatg ggcgaaagat ggcaacaact
    32941 tcgtctacat tgagcgggtt aaaggtgacg aggagttagg tggcatcagt atttatgcct
    33001 ttaacgagaa tcgtcgtctg caatccgtac gttatgccgc taccgcgaag tttgacccgg
    33061 aacataaagt ctggcgtctg tcgcaggttg atgaatctga tctgaccaat ccgaaacaga
    33121 ttaccggttc gcagacggtg agcggcacct ggaaaaccga cctcacgcca gacaaactgg
    33181 gcgtggtggc gctggacccg gatgcactct ccatcagtgg tttgcacaac tacgtgaagt
    33241 atctgaagtc gagcggtcag gatgccggac gttatcagct caacatgtgg agcaaaatct
    33301 tccagccgct atccgtggcg gtgatgatgc tgatggcgct gtcgtttatc tttggcccac
    33361 tgcgtagcgt accgatgggc gtgcgtgtgg tcacaggtat cagcttcggt tttgtcttct
    33421 acgtactgga ccagatcttc ggcccgctga cgttggttta cggcatcccg ccgatcatcg
    33481 gcgcactgtt gccaagtgcc agcttcttct taatcagcct gtggctgtta atgagaaaat
    33541 cgtaatctaa caagggagcg aaagctccct ttattattgt tgacaaagtc aaatacgagt
    33601 tcgaacggtc ccctcgcccc tttggggaga gggttagggt gaggggcaca gatcacgtct
    33661 gacttatctt tttctccccc ccagcaaact ccccaacatc ccgcgtacaa tctgattcgt
    33721 cacctggcga gcggcgcttt tcgccattgt ttgcaccaca ccatctttct tcccgccgcg
    33781 tggtccggta gtgccaaaca aaatatcctt caatccacca agaataccgt catccaccgc
    33841 cacctctttc cctttcacgg ggggattatt ttgctgctcg gtactggcct gaaagccttt
    33901 ttgcagcatc tcataggcgg actcgcggtc cacctcatct tcatatttgc catacaccgg
    33961 ggagtgatta atcaggccat tacgctcatc ttccgtcacc ggccccattc gcgaacaagg
    34021 tgcgatcacc atcgcccgtt ccaccacaga aggacttcct tttgcatcga gaaacgagat
    34081 taacgcctcg ccggtcccca gttcctggat tgccttttcg gtatcaaatg ccggattggc
    34141 ccgcatggtt tgcgccgctg tcttcactgc tttctgatct tttggcgtaa aagcccgcaa
    34201 agcgtgctga acacgattac cgagctggcc gagcacatta tccggaatat cagacgggtt
    34261 ttgcgaaacg aaccagacgc ccacgccttt tgagcgaata agccttatca cctgctcaat
    34321 cttatccagc agtacctgcg gtgcatcgtt aaacagcaga tgtgcctcgt cgaagaaaaa
    34381 caccagtttt ggtttctcca gatcgcccgc ttccggcaat tgttcataca actctgaaag
    34441 catccacagc aggctggcgg cgtacagttt cggcatctga taaagtttct cggcgctgag
    34501 gatattgata acgcctttac cgttggcatc ggtgcgcatc cagtctttga tatccagcat
    34561 cggctcacca aagaagtgtg ctgcgccttg ctgttccagc gacagtaatc cgcgctggat
    34621 ggcaccaacc gatgcgctac tgatatttcc gtactgattc tggaaggatt tggcgttatc
    34681 gccgatgtac tgggtaatcg cccgcaaatc tttaaagtcg agcagcaaca atccctgatc
    34741 gtccgcaatg cggaagataa tattcagcac gccagattgc acatcgttga gattcaacag
    34801 tcgcgccagc aacagcggcc ccagatccga aaccgtcgcc cgcaccgtgt ggcctttctc
    34861 gccaaagata tcccacacca ccaccggatt ggcatgcggt tgccagtcat tgacgccgat
    34921 atttttaagc cttgtgagca gtttttccga cgccgttcct gcctgcgcga taccggtcag
    34981 atcgcctttc acatcagcca taaacaccgg cacgccgatt tccgacaatg actctgccag
    35041 tttttgcagc gtaacggttt tacccgtccc cgttgcgcca gtaatcagcc cgtgacggtt
    35101 agccattccc ggcagtaaaa acagttctgt gtccggcgtg cgggcaatta acaggggttc
    35161 gctcatgaga tttcctccag tttcctgcct ggagtatagg caacggctgt gcactcttca
    35221 ccgctaaatt cagcagattg cgagacgcct tccaaagaga tttaagtgcc gggcgacagc
    35281 acccggcaaa gggatcagaa gtcggctttc aggactactc ggtagcgcgc tttaccatcg
    35341 cgtacatgct gaatggcgtc gttaattttc gacatcggga acagttcggt tgtcggcgca
    35401 accttgctgc gggcggcaaa gcgcatcagc tttcgcagct cataaggcgt gccggtagca
    35461 gaaccagaga tgctgcggtc gcccgcaatt aacgtaaagg ccggaacaga cagcggcgtg
    35521 agaaccgcac cgaccgtatg gaaattaccg ccataggtca gcgcctcaaa ataaggctgc
    35581 cagtcgaggc tgacgttcac agtattgata atgagatcaa actgccccga cagggctttc
    35641 agtgcctgcg gatcgcggct attcaccact ttatcggcac ccatcgccag tacttcctgc
    35701 tctttcgccg gattagaact aaaggccgtc acctcgcatc ccattgcgtg cagaagtttt
    35761 atagcgatat gccccagccc gccaatacca attaccccaa cgcggctggt agcagtgata
    35821 tggtgcatca acagtggttt aaagaccgtg ataccgccgc ataacagcgg cccggcggac
    35881 tcgatatcaa tattttctgg cagtggaatc acccattgcc agtccgcacg caacttctcg
    35941 gcaaagccac cgcgattcat aatcgtcggc accgcacctt gctcgcagtt gatctgatta
    36001 ccgctaatac aggcgtcgca gtgaccacag ctacgcgccg tccagccaat cccgacacgc
    36061 tgaccgacct gcaaaccttt atcctgcgcg gcgctcccga gtgccaccac gcgcccaatc
    36121 acctcatgcc cggcaaccag cggatattgt gaaaatcccc attcgttatc gatcatcgac
    36181 agatcggaat gacaaatccc gcagtaatcc acctgcactt caacatcttg tggcttcagc
    36241 tcaccgggat cgtactcata aacttccagt tcgccgcccg cttcttttgc ggcatagctt
    36301 tttatcatcg acatttttgg gtccttctct ggtgttgttt gggaagtgta gagcatggca
    36361 gggcggggtg cctggagtgt gacaaaggtt acatatcgct gagtgtaatg ctgaaaattt
    36421 cagcacttag cgaggtgggt gcaagctggc gcttgcatgg tggcgtgcga caggtataat
    36481 ccacaacgtt ttccgcatac ctcttcagtg ccgaagtggc gaaatcggta gacgcagttg
    36541 attcaaaatc aaccgtagaa atacgtgccg gttcgagtcc ggccttcggc accatcggaa
    36601 catcaataga cgtcaacgga cgtctttttt tgtgcctaaa atccagtatc cgcaaggctt
    36661 tcctcgcttt ttcactcaac ctaagtcaac ctgattcaat ctacatcaac ttactgatgc
    36721 gggtacaact gcgggtattt cccggttcga taatgtttgt acccacacag accccttgaa
    36781 aggatactca tcatggctct gagtgatgtg aaggttcgtt cggctaagcc tgaagcaaaa
    36841 gcctataagc tgaccgacgg tgaaggcatg gttttgctgg ttcaccctaa cggctccaaa
    36901 tactggcggc tacgttatcg ctttggcggc aaagagaaga tgctggcatt ggggaaatac
    36961 cccgaagtgt cgttggcgga tgccagggca cgccgggatg aggctcgtaa gcttttagct
    37021 aatggtgtcg atcccagtga aaacaagaaa gctgttaagg tagaacagga acaagaggcg
    37081 ataacgtttg aagtagtcgc cagagactgg cacgccagca atcagaagtg gtcggcatcg
    37141 catagcgctc gtgttttgaa aagtctggag gataatctct ttactgctat cggtaagcgg
    37201 aacattgcgg aactgaagac gcgggatctt cttgtaccca ttaaagcagt cgagtcatcc
    37261 gggcggctcg aagttgccgc ccgtttacag cagcgtacta ccgcgattat gcgctttgcc
    37321 gttcagagcg gcttaatcga ctacaacccc gcgcaagaga ttgcaggtgc ggttgctacg
    37381 gcgaaaagac agcaccgtgc agctcttgaa ctgaaccgta tacctgaatt acttcatcgc
    37441 atagatcact attctggcag accgttaact cgactggcgg ttgaactcac tttattggtt
    37501 ttcatccgtt cgagcgagct gcgttttgcc cgctggtcag aagtagattt tgaaacggct
    37561 atgtggacaa tcccaggtga gcgtgagcaa ctggaaggag tcaagcattc tcagcgtggc
    37621 tcgaagatgc ggacaccaca ccttgtacct ttgtcgcggc aggccctaag tattttggaa
    37681 aagatcaaaa gcatgagcgg aaatcgagag ctgatttttg tgggcgatca cgatccacgt
    37741 aagccgatga gtgagaatac ggtgaacaag gctttgcgcg taatggggta tgacacgaag
    37801 gtcgaggttt gtgggcatgg cttcaggacg atggcttgta gttcgttgat tgagtcggga
    37861 ttatggtcga gggatgcggt agagcggcag atgagtcacc aggagcgtag ctctgtgcga
    37921 gcagcttaca tccataaggc ggaacatctt ggggagcgta ggttgatgtt tgaagtggca
    37981 cactgaattt ggccacctga acagaggtga tatgctcacc tca
//
LOCUS       DADLSA010000023        36323 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.023, whole genome shotgun sequence.
ACCESSION   DADLSA010000023 DADLSA010000000
VERSION     DADLSA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.023"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(29..208)
                     /locus_tag="JN691_001189"
     CDS             complement(29..208)
                     /locus_tag="JN691_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAY7242682.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(292..1176)
                     /gene="yhdJ"
                     /locus_tag="JN691_001190"
     CDS             complement(292..1176)
                     /gene="yhdJ"
                     /locus_tag="JN691_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY7242683.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1262..1558)
                     /gene="fis"
                     /locus_tag="JN691_001191"
     CDS             complement(1262..1558)
                     /gene="fis"
                     /locus_tag="JN691_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAY7242684.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1584..2549)
                     /gene="dusB"
                     /locus_tag="JN691_001192"
     CDS             complement(1584..2549)
                     /gene="dusB"
                     /locus_tag="JN691_001192"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAY7242685.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2878..3759)
                     /gene="prmA"
                     /locus_tag="JN691_001193"
     CDS             complement(2878..3759)
                     /gene="prmA"
                     /locus_tag="JN691_001193"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAY7242686.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLYDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRNNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3771..5222)
                     /gene="panF"
                     /locus_tag="JN691_001194"
     CDS             complement(3771..5222)
                     /gene="panF"
                     /locus_tag="JN691_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAY7242687.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5212..5454)
                     /locus_tag="JN691_001195"
     CDS             complement(5212..5454)
                     /locus_tag="JN691_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAY7242688.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5563..6912)
                     /gene="accC"
                     /locus_tag="JN691_001196"
     CDS             complement(5563..6912)
                     /gene="accC"
                     /locus_tag="JN691_001196"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAY7242689.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6923..7393)
                     /gene="accB"
                     /locus_tag="JN691_001197"
                     /gene_synonym="fabE"
     CDS             complement(6923..7393)
                     /gene="accB"
                     /locus_tag="JN691_001197"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAY7242690.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8371..9345)
                     /locus_tag="JN691_001198"
     CDS             complement(8371..9345)
                     /locus_tag="JN691_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7242691.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9497..11437
                     /gene="csrD"
                     /locus_tag="JN691_001199"
     CDS             9497..11437
                     /gene="csrD"
                     /locus_tag="JN691_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAY7242692.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSIPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNARGQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11742..12785
                     /locus_tag="JN691_001200"
     CDS             11742..12785
                     /locus_tag="JN691_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAY7242693.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12851..13954
                     /gene="mreC"
                     /locus_tag="JN691_001201"
     CDS             12851..13954
                     /gene="mreC"
                     /locus_tag="JN691_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAY7242694.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13954..14442
                     /gene="mreD"
                     /locus_tag="JN691_001202"
     CDS             13954..14442
                     /gene="mreD"
                     /locus_tag="JN691_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAY7242695.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14451..15044
                     /gene="yhdE"
                     /locus_tag="JN691_001203"
     CDS             14451..15044
                     /gene="yhdE"
                     /locus_tag="JN691_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAY7242696.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15034..16503
                     /gene="rng"
                     /locus_tag="JN691_001204"
     CDS             15034..16503
                     /gene="rng"
                     /locus_tag="JN691_001204"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAY7242697.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16571..20371
                     /locus_tag="JN691_001205"
     CDS             16571..20371
                     /locus_tag="JN691_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAY7242698.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20527..21972
                     /gene="tldD"
                     /locus_tag="JN691_001206"
     CDS             20527..21972
                     /gene="tldD"
                     /locus_tag="JN691_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAY7242699.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22100..23029)
                     /gene="aaeR"
                     /locus_tag="JN691_001207"
     CDS             complement(22100..23029)
                     /gene="aaeR"
                     /locus_tag="JN691_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAY7242700.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23212..23415
                     /locus_tag="JN691_001208"
     CDS             23212..23415
                     /locus_tag="JN691_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAY7242701.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23423..24355
                     /gene="aaeA"
                     /locus_tag="JN691_001209"
     CDS             23423..24355
                     /gene="aaeA"
                     /locus_tag="JN691_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HAY7242702.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24361..26328
                     /gene="aaeB"
                     /locus_tag="JN691_001210"
     CDS             24361..26328
                     /gene="aaeB"
                     /locus_tag="JN691_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAY7242703.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPRDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26420..26692
                     /locus_tag="JN691_001211"
     CDS             26420..26692
                     /locus_tag="JN691_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242704.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26748..27011)
                     /gene="yhcN"
                     /locus_tag="JN691_001212"
     CDS             complement(26748..27011)
                     /gene="yhcN"
                     /locus_tag="JN691_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAY7242705.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27376..27846)
                     /gene="argR"
                     /locus_tag="JN691_001213"
     CDS             complement(27376..27846)
                     /gene="argR"
                     /locus_tag="JN691_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAY7242706.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28281..29219
                     /gene="mdh"
                     /locus_tag="JN691_001214"
     CDS             28281..29219
                     /gene="mdh"
                     /locus_tag="JN691_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAY7242707.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29281..30348)
                     /gene="degS"
                     /locus_tag="JN691_001215"
     CDS             complement(29281..30348)
                     /gene="degS"
                     /locus_tag="JN691_001215"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAY7242708.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30438..31805)
                     /gene="degQ"
                     /locus_tag="JN691_001216"
     CDS             complement(30438..31805)
                     /gene="degQ"
                     /locus_tag="JN691_001216"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HAY7242709.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31959..32357)
                     /locus_tag="JN691_001217"
     CDS             complement(31959..32357)
                     /locus_tag="JN691_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAY7242710.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32551..33678
                     /locus_tag="JN691_001218"
     CDS             32551..33678
                     /locus_tag="JN691_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAY7242711.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33897..34325
                     /gene="rplM"
                     /locus_tag="JN691_001219"
     CDS             33897..34325
                     /gene="rplM"
                     /locus_tag="JN691_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAY7242712.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34341..34733
                     /gene="rpsI"
                     /locus_tag="JN691_001220"
     CDS             34341..34733
                     /gene="rpsI"
                     /locus_tag="JN691_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAY7242713.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35136..35774
                     /gene="sspA"
                     /locus_tag="JN691_001221"
     CDS             35136..35774
                     /gene="sspA"
                     /locus_tag="JN691_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAY7242714.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35780..36277
                     /gene="sspB"
                     /locus_tag="JN691_001222"
     CDS             35780..36277
                     /gene="sspB"
                     /locus_tag="JN691_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAY7242715.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
BASE COUNT         8739 a         9055 c         9593 g         8936 t
ORIGIN      
        1 aaacgaatga attaagtgtc atgtaaattc acattctgtc ctgaaaattc agttcgtcac
       61 agttaaccag ttttagtgga ttaactgagt tcatattacg gcatttgtct gtttctgttg
      121 tcagcagttc aatccactgc atcagtaaag tatcaaacag aaaaacgaag acagcgaaaa
      181 cgacgagcca ccaatacttg cgaatcatta cctgagcctg tgcatgttta accaagtctg
      241 gcaggaaata tacacgaaat gaataatctg aaaaccaggc ttacacttcc tttactttgc
      301 aatgagatcg gggtcaactt cgcttaaccg gcttcgtttt gacaggttgc cggtctttct
      361 ttttttcact ttcgccagtt cttccgcgga gtaatgcgac gcgacatcca gccgtcgaag
      421 ccccattttg atgtactcgc tgttgatctc aataccaatg aattttcgtc cgctggcgat
      481 ggctacggca ccggtagtaa agctaccggc aaacgggtcg agaacgatat cgcctgggtt
      541 ggaagaggcg agaataatgc gtttcagtaa ggctttcggt ttttgcgtcg ggtggttctc
      601 atattcatcc atcaaataac gcacgcgcgg aaaatcccag acgttacccg gtactttttg
      661 atgattgtat ggctgtggag gatttttgcg ataatcgatc aacgcgcgct gcgatccggt
      721 tttggcttct accagaatag catcaccgtt gaatgtgtag ttctttgcgt ctttcaccat
      781 catcaggatg ggttcgtaca tggagccgta gtgttttttc gcctgtactc cagaactgtc
      841 atatgaccag acgatgcgac ttttgatggt aaaaagcttg cggcactgga gatcgataaa
      901 gggcatgttt tccgtactgt tcataatgta catgctgccc tgctttttca gaacgcggtg
      961 gcactctgca atcacttcaa acagccagtc gataaacaga tcttctttcc aggcttcgat
     1021 cagaccatca aaatttttac cgatgttata cggtgggtcg gcaaagagca gatcgacgct
     1081 ttcagtaggt agctttttaa gttcggcaag ggcatcaccg tgaataatgg tcttagcttc
     1141 attaccaaac cgggtcggtt cacatcctgt tctcatggtc actccctttg tgacacctat
     1201 aaaaaaggcg cttccccatg ccgagtagcg cctttttaat caagcattta gctaacctga
     1261 attagttcat gccgtatttt ttcaattttt tacgcagcgt accacggttg atgcccatca
     1321 tcagcgcagc acgggtctgg ttaccacggg tgtattgcat caccatgtcc aacaggggct
     1381 gttctacttc agccagtacc agctcataga ggtcattcac atcctgacca ttcagttgag
     1441 caaaatagtt cttcagtgcc tgtttaaccg agtcacgcag gggtttttgg gttacctgat
     1501 cctgagagtt aacggtagaa acggtcagta cgtcagaatt tacgcgttgt tcgaacatag
     1561 ttctgtcagc tctttatttc tgtttacgca aaattttcga agtatgcctc caacgcctcc
     1621 agctgttcgc tggcatcctc aatggcgttg aatgtgcgcc gaaactggtc atttggagcg
     1681 tgttcctgga gataccagga aacgtgttta cgtgcaattc ggtacccttt tgccggacca
     1741 taaaagtcat gcagttcccg aacgtgcgcg caaagcaagc gcttaacctc tgccaaaggc
     1801 agtgggggca gcaactcccc agtgtccaga taatgctgga tttcccgaaa gatccagggt
     1861 cttccctgag ctgcgcggcc tatcatcagg gcatccgccc ctgtatagtc gagcacagct
     1921 ctggctttaa gcgggtcagt aatgtcgcca ttcgcgataa ccggaatgga aactttctgc
     1981 ttaactgccc gaatactgtc gtactcagct tctccattga acaaacaggc gcgtgtacgg
     2041 ccatgaatgg tcagagcctg aatgccacag tcttcagcca gttgggcaat ctcttcgcag
     2101 ttacggtgtt ccggtgccca gccggtgcga atcttcaggg taacaggaac gtccactgca
     2161 ttgacgacct cggtaaggat cgatttaacg acatccgggt actgcaagag ggctgaacct
     2221 gcgagcttgc gattcacttt tttagccggg caacccatat tgatatcaat aatctgggca
     2281 ccgctttcca cgttaatacg tgctgcatct gccatttctt tcggagcgct accagcaatt
     2341 tgcacggtgc gaataccggg ttcatcaatg tgcaccatcc gtaaacgaga tttgtcgctt
     2401 tcccaaacct gtgggttaga agacatcatc tcggatactg tcaatccggc tcccatctcg
     2461 tagcacaacg tccgaaaagg tctgtctgta atgccagcca tgggcgctgc gatcaggcga
     2521 tttctgagct gatattgtcc gatgcgcatg agttaagaaa tgaccatact gtgactgcaa
     2581 ggcggcgtat attacgcatt ttttgcacga gatgaaaggc caaactttga ccaatcctct
     2641 gagatggatc aaagaattgc atttaaaatg agcgtggtgc gataattact cataaaaatc
     2701 atcatattag aaaatagtga ctaaaattta cactcaaaga aatttgagta agttctcaat
     2761 ttttctttat gaatgaaaat tttggcacgc aaatttgcgt aaataatcgg caaatttacg
     2821 tgccttttgt gagcttgctc gcacttcgcc ccgcgtcacc ctacgccgat gcgaaggtta
     2881 attcttacga ccggtaatac ggcaccactc ttctttttcc acgaccgggt ccagtgcgaa
     2941 gctatcggca taagcttcac aaacgctctc tgcctggctt gccagaatac cggaaaggcc
     3001 cagcaaaccg cctgaaaccg gcaggacgct gattaacggt gccagttcac gtaatgggcc
     3061 tgcaaggatg ttagcgacca ccacgtcggc tttcatttct tctggctgat ctttcggtaa
     3121 gtagagttcc agacggtcag aaacgccatt acgttcggcg ttattgcggc tggcctgaat
     3181 cgcctgcgga tcgatatcaa taccaatggc ttttgctgca cccagtttca gcgccgcgat
     3241 cgccagaatg ccggaaccac agccaaagtc gatgactgtt ttaccggtta aatcgaggct
     3301 gtcgagccat tgcaggcaca gagaggtggt tggatgggta cccgtaccaa acgccagccc
     3361 tggatctaac atcacgttga cggcgttttc gtccggcaca tcacgccagc tagggcagat
     3421 ccacagtcgt tcaccaaagc gcatcgggtg gaaattatcc atccattcgc gctcccagtc
     3481 tttatcttct agttgttcga ttttatgcgc gaagcctgcg ccgagcagcg gatggttttc
     3541 cagaatcgcc accacgtcgt tcatatcggt ttcagcgtcg tacagaccaa tcacatcggt
     3601 gtcgccccac aggcgcgttt cgcccggcag cggctcaaat actggcgtat cgtgggtatc
     3661 ctgaaaagtg atagaaacgg cacccgcttc catcagcgca tcgctaagat cttccgcgtt
     3721 cgcgccggtg gtgttcagtt tcagttggat ccaaggcatg gcaaaactct ttatttatca
     3781 gtagtcaaaa cggtagcttg tgggacggat gtaccgaaac ggtttccgac caggaaagcc
     3841 agcaaactta gtagtaacga gggcacgata gggtggaagc ccaggtactg aatattcagc
     3901 gtcgcgagta cggcatacag cacgccgcca acgatcatcg cacttagcgc gcctttggcg
     3961 ttggcgcgtt cccagtaaag acccagcacc agcggccaca ggaaaacggc ttccagccca
     4021 ccgaaggcca gcaaattcag ccagatgatc atttctggcg gcttccaggc ggcaagcagc
     4081 agcaacgcgc cgagaactaa cgtaattacc gccgacatcc gcttcagacg cgtctcgttt
     4141 tgcatttgat ccggacggat attcagatag agatctttaa tgatcgtagc ggaactttgc
     4201 agcagttggg cgttaattgt cgacatgatc gcagccatag gtgcagccag aaagatcccg
     4261 gcagcaaaag gtggcagcac ttttaccatt aacgtcggga tcaccagatc cggtacggtg
     4321 agatcgggga tcactgcacg acctaacgct ccggccaggt gcataccgaa catcaggatt
     4381 gcgactacaa tcgtaccgat gatgatcccc cggtgtactg ctttgctgtc tttataagag
     4441 atacagcgca ccgcagtatg cggcaggcca atcacgccaa agcacaccag tacccagaac
     4501 gacgtcataa aggcaggcga cagaatatcg tcagcgcctt gtggcgtaac cagttgcgga
     4561 tcgatggttt gcaaggtctg tactgcgttg cttaagccgc cagcggcatg taccacgcca
     4621 ataagcagca caacggtgcc aatcagcatc acaagccctt gcatggtgtc gttcagcacg
     4681 ctggcgcgaa agccaccaaa ggcggtatat aacgcaatgc tgataccaaa aatcagcagc
     4741 ccggtttcat aaggaatacc cgccgcggtt tccagcaggc gcgcaccgcc gataaactgc
     4801 acggtcatcg caccaacgaa cgcaaccagc aaactcaaac tcgccagcca caccagaaga
     4861 cgactctggt agcgggcaaa cagcatatcg ttcagcgtca ctgcattgta gcggcgcgca
     4921 agaatcgcaa acttcttgcc gagaataccg agtgaaagcc agacagcagg aagctgaatc
     4981 atcgccagca atacccagcc cagcccgtat ttataagcag ctcctggccc gccgataaac
     5041 gaactggcac tgatataggt cgcggtgagc gtcatcgcca gcacaatacc gcccatagag
     5101 cggctgccga ggaaatactc attaaggaag gtgccggtgc tccgtttacg catcgcataa
     5161 accgagatac cgaacaccac caccagatag gcgaccagcg gtagaattac ttcaagctgc
     5221 atcgtcatcc tccagtggga tatcgcgata gataaatttc accatcgccc agcacagtcc
     5281 aataaacagt agcggcgtca ggatgcaggc catctcaaac cagcgcggaa agccggtaaa
     5341 accgggggca acgccaggta agtaagcgtc tactaaccaa actgccagat acaaaagggt
     5401 cagccccagc gcccagcgcg cctctttatg ggcctgaaca aaacgagtgt ccattttttg
     5461 tccctgatgg gtgaagaaag cgaggattgt accttatggg ggttgtcgat ccccagtaat
     5521 aaaaaaggcc ggaaaatccg gccttttgac gcttttgcag tcttattttt cctgaagacc
     5581 gagttttttc tccagatagt ggatgttagt gccaccatgc tggaagttct cgtcattcat
     5641 gatgcggatc tgcagatcaa cgttggtttt gataccgtcg atgatcagct cctgcagcgc
     5701 attcttcatg cgggcaatcg ccacgtcacg gttttcaccg tagcaaatca gcttaccgat
     5761 cattgagtca tagtacggcg gtacggtgta gcccgcgtag atatgagact cccaacgtac
     5821 gccaaaaccg ccaggtgcgt ggaaacgggt gattttgcct gggcttggca ggaaggtgtt
     5881 cggatcttcg gcgttgatac gacattccac cgcatggccg cgaacgtgaa cttcttcttg
     5941 cttgatcgac agcggttgac cggcagcgat acgcagctgt tctttgatca ggtcaacgcc
     6001 ggtgatcatt tctgtaaccg ggtgttctac ctgaatacgg gtgttcattt cgatgaaata
     6061 gaactcgccg ttttcgaaca ggaactcgaa agtacctgca ccgcgatagc cgatatcaac
     6121 acacgcttta gcgcaacgtt cgccgatgta gcgacgcagt tccggggtaa tgcccggtgc
     6181 tggtgcttct tcgaccactt tctggtggcg gcgctgcatg gagcagtcac gttccgccag
     6241 atagatagcg ttgccctgac cgtcagccag tacctgaatc tcgacgtggc gaggattttc
     6301 caggtatttc tccatgtaaa ccatatcgtt gctgaaagca gctttcgctt ccgcacgggt
     6361 catggagatg gattgtgcca gttcagcgtc gccgcgcact acgcgcatac cgcgaccgcc
     6421 gccgccgccg gaggctttga taatcaccgg ataaccaatg cgtttagcaa tggcgcggtt
     6481 tttatccata tcgtcgccca gcgggccgtc agaaccaggt acgcaaggaa cgcccgcttt
     6541 tttcatcgcc gcgattgcgg atactttgtc gcccatcagg cgaatggttt ctgctttcgg
     6601 gccgatgaag ataaagccgg agcgttcaac ctgctcggca aagttggcgt tctcggagag
     6661 gaagccgtaa cccggatgga ttgctactgc gccggtgatt tcagcggcgc tgatgattgc
     6721 cgggatgttc agataacttt ttactgacgg agcagggcca atacagaccg tttcatctgc
     6781 cagtaatacg tgttttagat cgcgatccgc gctggagtgc acagcgacag tcttgatgcc
     6841 cagttcttta caggcacgaa gaatacgcaa tgcaatctcg ccgcggttgg caataacaat
     6901 tttatccagc atgttcgcct cgttactcga tgacgaccag cggctcgtca aattctaccg
     6961 gttgtccact ttcgaccaga attgctttca cggtaccgga tttgtccgct tcgatctggt
     7021 tcatcatttt catggcttca acgatgcaca gggtatcgcc cacgttgact ttctgaccca
     7081 cttcgatgaa cgcttttgcg tccgggcttg gggtgcggta gaaagtacca accatcgggg
     7141 aacgtacgat gtgaccactg atttccgctg ctgctggcgc ttccatggaa ggaacggtcg
     7201 ccggagcggc tgcgttagat tgagctggct gctgcatcat tggtgcagcg taagcttgtt
     7261 gcatcacagg gaaacttgcg gcaggagctg cacggctaat gcgtactgac tcttcgcctt
     7321 cagaaatttc cagttcggag atgcctgatt cttcaaccag ctcgatcagt tttttaatct
     7381 tacgaatatc catgagtggg ttccgtactc tttgtttagt gtgattgtga caggcgtttc
     7441 accgccgtct gtaaagcgta tgccccgaca gctgtatgca tagcgataaa ttccagcagg
     7501 ccgggacgcc ggtctatttt gccccagagt gtcaatgtta gacttgacgg acattgtgca
     7561 gcgatctgct gctatcctcc ggcaaaaaac aaaatatacc ttcgttgcgc caatgtcacc
     7621 ttttccgcag cgcaaaaact gcgtcaggga ggacgaggtc gcacattata acgatttcgt
     7681 agcaattggc agctaaatac tggtcttatc agggaagata atcaacagct aacatgtaaa
     7741 taaccttcaa caccgtgtaa tttgtaacaa gccgcacaat tcacgaaatt agcgccatca
     7801 tcgacggaac ttcttataac gtaaggtgaa aagctgaaaa gccagccctg cgaagatgag
     7861 gggctgcaat gagataatct tcacagacca caaataatgt agggatgcca ggatcgcgac
     7921 aagatagacg acattacgca gcggttgctg gggattaccc aattttcact gcatcgtctg
     7981 gatagatgtg aatgctaaag cacgcaaaat caaccaactg acaattctca gcattaaata
     8041 ggttcgagtg gttaatttgt agtcaaataa aatcagatta ttcaccccat ggtaccagaa
     8101 atcagtaaca ggttaagcgt agtgtcgccc aggcaaagca ccataaacct aacaggcgac
     8161 ggaggcgtat caataatggc tgtttagagg agtatcaatg cgcatcaatt tgtggtcagt
     8221 cgaaatcatg actttccagt ttggatgatg ttgtacaact tcctgaccaa ggtgttcgac
     8281 aaaagctgtc cggctaaccg cttttcccgt taaccgattg aataatgatg tcaaaatgag
     8341 ttccctctct tattattcct tgctaaatgg ttagttaacc ttcaccagcg tgcgaccctg
     8401 gatctggtta ttaatgatgg cctcggcaaa gttcggtgcc tctgacagag atatctcttt
     8461 tgccgcctgg gtatagaatg attccggtaa atcggcgacc agtcgctgcc aagcttgtgc
     8521 gcggcgttct ggtggcgtca ttactgaatc caccccttgc aaacggacat tacgcagaat
     8581 aaatggcatg accgtggttg gcagagtaaa accacccgcc agaccacagg ccgccacgca
     8641 gccgccgtaa ttcatttgcg ctagcacttt tgccagcact ttgtcgccaa cagtgtcaat
     8701 tgccccagcc cagacttgtt tttccagagg acgggattcg gcaaactcat cacgagggag
     8761 aatacggctg gcacctaaac ttttcagata ttcatgggta ctttcgcgac cggaaacggc
     8821 aacgacctga taacccaact tatgcagcag cgccacggcg gtactgccga cgccaccact
     8881 ggcacccgtc acgacaatct ccccgtcctg cgggcgaaca ccggcatctt ccagcgccat
     8941 cacacacagc atggcggtaa aaccggcagt accgataatc attgctttac gcgcgtccag
     9001 cccttgcggc atggcaacca gccagtcacc tttcactcgc gcctgctccg ccagcccacc
     9061 ccagtggttt tcaccaacgc cccagccagt gagtaacacc tcctgaccgg catgaaaacg
     9121 cggatcttcg ctggtgcgta cagttccggc aaaatcgatc ccaggaatca tcggaaaatt
     9181 acggatgatt tttcccttac cggtaatcgc cagcgcatct ttatagttca ggctcgacca
     9241 gtgaacatcg accgtgacat cgccctccgg caggcgactt tcgtccagag tctgtactga
     9301 tgcgagagtt ttgccgtcct gctgttctaa aagtaacgcc tgcataagtg gtcctcatgt
     9361 gcatgatgga ttggaaaata atttctgaag actatactcg ctaatggaaa agcaaagccg
     9421 atgaagcgca ataaattgcg agataaatct gatttgctag tatgcccgct tcctcactat
     9481 cggagttaac acaaggatga gattaacgac gaaattttcg gcctttgtta cgctgctcac
     9541 cgggttaaca atttttgtga ctttgctggg ctgttcgcta agtttctaca acgccattca
     9601 gtataagttt agtcatcgcg ttcaggcggt ggcgacggcg attgataccc accttgtgtc
     9661 gaatgacttc agcgcattaa ggccacaaat taccgaatta atggtgtcag cagatatcgt
     9721 tcgtgtagac ctgctgcatg gtgataaaca ggtttatacc ctggccagaa atggtagtta
     9781 tcgtccggtt ggcaccagcg atctgtttcg tgaactgagc attccgttga taaagcatcc
     9841 ggggatgtcg ttgcgtctgg tttatcagga tccgatgggc aactatttcc attcgttgat
     9901 gaccaccgcg ccgctcacgg gggcgattgg ctttatcatt cttatgctct ttctggcggt
     9961 acgctggtta caacggcaac ttgccgggca agaattgctg gaaacccggg ctactcgtat
    10021 cttaaacggc gagcgtggct ctaatgtgtt gggaaccatc tatgaatggc cgcccagaac
    10081 cagcagtgcg ctggatacgc tgcttcgtga aattcagaac gcacgcggac aacacagccg
    10141 tcttgatacg ctgatccgct cttatgccgc acaggacgtg aaaaccggcc tcaataaccg
    10201 actctttttc gataatcagt tagcaacgtt actggaagat caggagaaag taggtaccca
    10261 cgggatcgtg atgatgattc gtctgccgga tttcaatatg ttgagcgata cctgggggca
    10321 cagccaggtt gaagaacagt tcttcactct gacgaatctg ctgtcgacat ttatgatgcg
    10381 ctaccctggc gcactgctgg cgcgttacca ccgcagtgat tttgctgcgc tgttaccgca
    10441 ccggacgtta aaagaggcag agagcatagc cagtcagtta atcaaagccg tcgatacctt
    10501 gccgaacaat aaaatgctcg atcgcgacga tatgatccac attggtatct gtgcctggcg
    10561 tagtggtcag gataccgagc aggtaatgga acatgcagag tctgccacgc gtaatgcggg
    10621 attgcagggt ggcaatagct gggctattta tgatgactcg ttgcctgaaa aaggacgcgg
    10681 taatgttcgc tggcgtacgc ttatcgagca aatgctcagt cgcggcggcc cgcgccttta
    10741 tcaaaaaccg gcggttactc gcgaaggtca ggttcatcat cgcgaactca tgtgccgcat
    10801 cttcgatggt aatgaagagg ttagctcggc ggagtatatg ccgatggtct tgcagtttgg
    10861 cttatcggaa gagtatgacc gtctgcaaat cagccgtctt attccactat tgcgttactg
    10921 gccagaggaa aatctggcga ttcaggttac cgttgagtcg ctgattcgtc cgcgttttca
    10981 gcgttggctg cgcgatacgt taatgcaatg tgaaaaatca caacgaaaac gcataattat
    11041 tgaacttgca gaggccgatg taggtcaaca tatcagtcgt ttacaacctg ttattcgttt
    11101 agtgaatgct ttaggggtac gggtagccgt caaccaggct ggtttgacgc tggtaagcac
    11161 cagttggatc aaagaactta atgttgagtt actcaagctc catccggggc tggtcaggaa
    11221 cattgagaag cgaacggaga accagctgct ggttcaaagc ctggtggaag cctgctccgg
    11281 gaccagcacc cacgtttacg ccaccggcgt gcgttcgcga agcgagtggc agaccctgat
    11341 tcagcgcggt gttacaggcg ggcaagggga ttttttcgcg tcctcacagc cacttgatac
    11401 taacgtgaaa aaatattcac aaagatactc ggtttaacct gccgtttaat ccgttttcac
    11461 gtagaataac gcgcgctgcg tctcatggga gtgtgcttgt ctgctcgcca gattgttgca
    11521 gcacatatgc agatgaatga ccttacgcgg ttgcaaacag gcgaggaatg ctgctgatgc
    11581 attaagcctt tctggactca ggcagagatt tgtaacaaag gaaacgaact gcactaattt
    11641 tcaccgtagc agatgatttt tgcgccttgt cgctgctgcg tgtggttggt aaagtaagcg
    11701 gattttcttt tccgccccag ctttcaggat tatcccttag tatgttgaaa aaatttcgtg
    11761 gcatgttttc caatgacttg tccattgacc tgggtactgc gaataccctc atttatgtaa
    11821 aaggacaagg catcgtattg aatgagcctt ccgtggtggc cattcgtcag gatcgtgccg
    11881 gttcaccgaa aagcgtagct gcagtaggtc atgacgcgaa gcagatgctg ggccgtacgc
    11941 cgggcaatat tgctgccatt cgcccaatga aagacggcgt tatcgccgac ttcttcgtga
    12001 ctgaaaaaat gctccagcac ttcatcaaac aagtgcacag caacagcttt atgcgtccaa
    12061 gcccgcgcgt tctggtttgt gtaccggttg gcgcgaccca ggttgaacgc cgcgcaattc
    12121 gtgaatccgc gcagggcgct ggtgcccgtg aagtcttcct gattgaagaa ccgatggctg
    12181 ccgcaattgg tgctggcctg ccggtttctg aagcgaccgg ttctatggtg gttgatatcg
    12241 gtggtggtac cactgaagtt gctgttatct ccttgaacgg cgtggtttac tcctcttccg
    12301 tgcgcattgg tggtgatcgt ttcgacgaag ctatcatcaa ctatgtgcgt cgtaattacg
    12361 gttctctgat cggtgaagca accgcagaac gtatcaagca cgaaatcggt tcggcttacc
    12421 cgggcgatga agtccgtgaa atcgaagttc gtggccgtaa cctggcagaa ggtgttccac
    12481 gcggttttac cctgaactcc aacgaaatcc tcgaagcact gcaggaaccg ctgaccggta
    12541 ttgtgagcgc ggtaatggtt gcactggaac agtgcccgcc ggaactggct tccgacatct
    12601 ccgagcgcgg catggtgctc accggtggtg gcgcactgct gcgtaacctt gaccgtttgt
    12661 taatggaaga aaccggcatt ccagtcgttg ttgctgaaga cccgctgacc tgtgtggcgc
    12721 gcggtggcgg caaagcgctg gaaatgatcg acatgcacgg cggcgacctg ttcagcgaag
    12781 agtaatcgga tgcaggcagg ggaagcgtct gtttaccctg cctggtctga tacgagaata
    12841 cgcataactt atgaagccaa tttttagccg tggcccgtcg ctacagattc gccttattct
    12901 ggcggtgctg gtggcgctcg gcattattat tgccgacagc cgcctgggga cgttcagtca
    12961 aatccgtact tatatggata ccgccgtcag tcctttctac tttgtttcca atgctcctcg
    13021 tgaattgctg gatggcatat cgcagacgct ggcctcgcgt gaccaactag aacttgaaaa
    13081 ccgggcgtta cgtcaggaac tgttgctgaa aaacagtgaa ctgctgatgc ttggacaata
    13141 caaacaggag aacgcgcgtc tgcgcgagct gctgggttcc ccgctgcgtc aggatgagca
    13201 gaaaatggtg actcaggtta tctccacggt taacgatcct tatagcgatc aagttgttat
    13261 cgataaaggt agcgttaatg gcgtttatga aggccagccg gtcatcagcg acaaaggtgt
    13321 tgttggtcag gtggtggccg tcgctaaact gaccagtcgc gtgctgctga tttgtgatgc
    13381 gacccacgcg ctgccaatcc aggtgctgcg caacgatatc cgcgtaattg cagccggtaa
    13441 cggttgtacg gatgatttgc agcttgagca tctgccagcg aatacggata ttcgtgttgg
    13501 tgatgtgctg gtgacctccg gtctgggcgg tcgtttcccg gaaggctatc cggtcgcggt
    13561 tgtctcttcc gtaaaactcg atacccagcg cgcttatact gtgatccagg cgcgtccgac
    13621 tgcaggactg caacgtttgc gttatctgct gctgctgtgg ggcgcggatc gtaacggcgc
    13681 taacccgatg acgccggaag aggtgcatcg tgttgctaat gaacgtctga tgcagatgat
    13741 gccgcaggta ttgccttcgc cagacgcgat ggggccaaag ttacctgaac cggcaacggg
    13801 gatcgctcag ccgactccgc agcaaccggc gacaggaaat gcagctactg cgcctgttgc
    13861 gccgacacag cccgctgcta atcgctctcc acaaagggct acgccgccgc aaagtggtgc
    13921 tcaaccgcct gcgcgtgcgc cgggagggca atagtggcga gctatcgtag ccagggacgc
    13981 tgggtaatct ggctctcttt cctcattgcg ctgttgctgc aaatcatgcc ctggccggat
    14041 aacctgattg ttttccggcc aaactgggtg ttacttatct tgttgtattg gatcctggcc
    14101 ttgccccatc gcgtaaatgt gggcacaggt tttgtgatgg gtgccatact ggatctgatc
    14161 agcggctcga tgcttggcgt acgcgtattg gcgatgagca tcattgctta cctggtggcg
    14221 ctgaaatacc agcttttccg caacctcgca ttatggcagc aggcgctggt cgtcatgttg
    14281 ctttcgctgg tggtggatat tattgttttc tgggcagagt ttttagtgat taacgtctct
    14341 ttcagaccgg aagtgttctg gagtagtgta gtcaatgggg tgctctggcc gtggattttc
    14401 ttgctgatgc gtaaagtccg tcagcagttt gcagtgcaat aaaggtttct atgacttctc
    14461 tgtatttagc ttccggttct ccgcgtcgtc aggagttact tgcgcaactt ggcgtgacct
    14521 ttgaacgtat tgttacgggc attgaggagc agcgtcagcc gcaggagagc gcgcagcagt
    14581 atgttgtgcg tctggcacgc gagaaagcac aggcgggtgt cgcgcaaacg gcgcaggatc
    14641 tcccggtgct gggtgcggat attatcgtta tcctgaacgg agaagtgctc gagaaaccgc
    14701 gcgacgcaga gcatgcggcg cagatgttgc gcaaattatc gggtcagacc catcaggtga
    14761 tgacggcagt ggcgttggcc gacagccagc acattctcga ttgcctggtg gtcaccgatg
    14821 tgactttcag aacgttaaca gacgaagaca tcgcgggcta tgtcgccagc ggtgaaccgt
    14881 tagataaagc aggtgcatac ggtattcagg ggctgggtgg ctgttttgtc aggaagataa
    14941 atggcagcta tcacgccgta gtcggcttac cgctggttga aacgtatgaa ttattaagta
    15001 attttaacgc actgcgtgag aaaagggata aacatgacgg ctgaattgtt agtaaacgta
    15061 acgccttcgg aaacgcgagt ggcgtatatt gatggcggta ttctgcagga aattcatatt
    15121 gaacgtgagg cgcgacgcgg aatagtaggc aatatctaca agggtcgtgt aagtcgtgta
    15181 cttccgggta tgcaggcggc ttttgtagat attgggctgg ataaagccgc gtttcttcat
    15241 gcatccgaca tcatgccgca taccgaatgt gtggcgggtg aagaacaaaa gcaattcacg
    15301 gtgcgcgaca tctcggaact ggttcgtcag gggcaagatc tgatggtgca ggtggtgaaa
    15361 gatccgcttg gcactaaagg tgcgcgcctg accaccgata tcacgctgcc ttctcgctat
    15421 ctggtattta tgccaggggc ttctcacgtt ggggtttccc aacgaattga aagcgaatca
    15481 gaacgtgaac gcctgaaaaa agtggtcgca gagtattgcg acgagcaggg cgggtttatc
    15541 atccgcaccg cagcggaagg ggttggcgag gctgaactgg cctccgatgc cgcttatctg
    15601 aaacgcgtct ggaccaaagt tatggagcgt aaaaagcgcc cgcagacccg ttatcagctg
    15661 tacggcgaac tggcgctggc gcagcgtgtt ctgcgtgatt ttgccgacgc cgaactggac
    15721 cgcattcgcg ttgactcacg cctgacttac gaagcattgc tggagtttac ctcggagtac
    15781 attcccgaga tgaccagcaa gctggagcat tacaccggac gccagccgat tttcgatctc
    15841 tttgatgtcg aaaacgaaat ccagcgagcg ttggaacgca aagtagaact gaaatccggt
    15901 ggttatctga ttatcgacca gaccgaagcg atgaccaccg tggacatcaa taccggagcg
    15961 tttgtcggtc atcgcaatct ggacgacact attttcaata ccaatattga agcgacgcag
    16021 gctatcgctc gccagttacg gttgcgtaat ctgggcggga ttatcattat tgatttcatc
    16081 gatatgaata atgaagatca ccgccgccgc gtgctgcatt cgctggagca ggcgttgagc
    16141 aaagaccggg tgaaaaccag cgttaatggt ttttcggcgc tggggctggt ggagatgacg
    16201 cgtaaacgca cccgcgaaag cattgagcac gtactgtgta acgaatgccc aacctgccac
    16261 ggtcgcggaa cggtgaaaac cgtggaaacg gtatgctatg aaatcatgcg cgagattgtt
    16321 cgtgtccacc atgcttacga ctccgaccgt ttcctggtct atgcttctcc ggcagtagcc
    16381 gaagccttga aaggcgaaga gtcacactcg ctggcggaag tggaaatttt cgttggcaaa
    16441 caggttaaag tacaaattga accgctctat aaccaggagc agtttgacgt cgtaatgatg
    16501 taaacagatg ctgggccgcc atccggcaaa gggtttttga gtcacatttt tagcagacaa
    16561 ggagtgacgg gtgaggcgat tgccggggat tttactgctt actggagccg cgctcgttgt
    16621 gatcgctgcc ctgctggtta gcggcctgcg tattgcttta ccgcatcttg acgcctggcg
    16681 tccggaaatc ctcaacaaaa tagaatccgc gactggcatg ccggtagaag ccagtcagct
    16741 ctcagccagc tggcagaatt ttggcccgac gcttgaagca cacgacatcc gtgcagaact
    16801 aaaagatggc ggcgaatttt cggttaaacg cgttactctg gcgctggatg tctggcagag
    16861 cctgttacat atgcgctggc agtttcgcga cctcactttc tggcagctgc gctttcgcac
    16921 caacactcct atcaccagcg gtggtagcga tgacagtctg gaagccagtc acatcagcga
    16981 tctgtttctt cgtcaatttg accatttcga tctccgcgac agtgaagtca gttttctgac
    17041 gccatccggt cagcgcgccg agctggcgat cccacaactc acctggctga acgatccacg
    17101 tcgacaccgt gcggaaggcc tggtaagcct ctccagcctt accggacagc acggcgtgat
    17161 gcaggtgcgc atggatttgc gcgatgatga ggggttgtta agcaatggtc gcgtctggct
    17221 acaggcggat gacatcgacc tgaagccgtg gctcggtaaa tggatgcagg acaatattgc
    17281 tctggaaacg gcacagttct cccttgaagg ctggatgacg atcgacaaag gcgatgtaac
    17341 cggcggtgac gtctggctga aacagggcgg tgccagctgg ttgggcgaga agcaaacgca
    17401 tacgctgtcg gtggataatc tgaccgcgca tattacgcgt gaaaatccgg gctggcagtt
    17461 ctctattccc gatacacgga tcacgatgga cggcaaaccc tggccgagcg gagcattgac
    17521 gctggcctgg ataccggaac aggacgttgg cggcaaagac aataaacgca gtgacgaact
    17581 ccggattcgc gctagtaatc tggagctggc aggcctggag ggcatacgcc cgctggccgc
    17641 gaaactttca cctgcactgg gtgatgtttg gcgctccaca caaccgagcg gcaagattaa
    17701 cactctggcg ctggatatcc cgcttcaggc ggcagacaag acccgttttc aggcatcgtg
    17761 gagcgatctg gcctggaagc aatggaaatt attaccgggt gcggaacact tctccgggac
    17821 gctttccggc agcgttgaaa atggtttgct taccgcgtcg atgaagcagg caaagatgcc
    17881 ttacgaaacg gtattccgtg cgccactgga aatcgccgac ggccaggcaa ctataagctg
    17941 gctgaacaat gacaaaggtt tccagctgga tgggcgtaat attgacgtta aagccaaagc
    18001 cgtccatgcg cgcggcggtt ttcgttacct gcaacctgct aacgatgaac cctggctggg
    18061 tattctggct ggcatcagta ccgatgatgg ttcacaagcc tggcgctatt tcccggaaaa
    18121 cttgatgggt aaagacctgg ttgattattt aagtggtgcg attcagggcg gtgaagcgga
    18181 taacgcgacg ctggtttatg gtggcaatcc gcaactcttc ccctataaac acaacgaagg
    18241 tcagtttgaa gtgctggtgc cgctgcgcaa cgcgaagttt gccttccagc cggactggcc
    18301 tgcattaact aaccttgata ttgaactgga ctttattaac gacggtttat ggatgaaaac
    18361 cgatggcgtt aatctgggcg gcgtgcgcgc gagtaatctc accgcagtga tccctgacta
    18421 ctcaaaagaa aaactgctga ttgacgctga cattaaaggt ccgggtaaag ccgttggccc
    18481 ttactttgat gagacaccgc tgaaagattc tctgggtgcg accctgcaag aactccagct
    18541 cgacggcgat gtgaatgctc gcttacatct tgatatcccg ctgaacggcg aactggtaac
    18601 cgcgaaaggt gaagtgacgc tgcgtaataa cagtctgttt atcaaaccac tcgacagcac
    18661 cctgaaaaat ttgagcggta aattcagctt tatcaatggc gatctgcaaa gtgaaccact
    18721 gacagcaagc tggtttaatc agccgttgaa cgtggatttt tccaccaaag aaggggcaaa
    18781 agcctaccag gttgcggtga atctcaacgg taactggcaa ccggcgaaaa ccggcgttct
    18841 gcctgaagcg gtgaacgaag cattgagtgg cagcgtggcg tgggatggta aagtgggcat
    18901 tgatctgcct tatcatgctg gcgcgaccta taacgtagag ctgaacggcg atctgaataa
    18961 tgtgagtagt cacttacctt caccgttagc caaacctgcg ggtgaaccac tagcggtaaa
    19021 cgttaaggtt gatggcaatc tcaacagctt tgaattaacc ggacaggctg gtgcggataa
    19081 ccatttcaat agccgctggt tgcttggtca aaagctgacg ctcgatcgtg ctatttgggc
    19141 ggcagacagt aaaacgctcc cgccgttgcc ggaacaaagt ggcgttgaac ttaatatgcc
    19201 gccgatgaat ggtgccgagt ggctggccct gttccagaaa ggcgctgcgg agagtgtcgg
    19261 tggtgcagcg agtttcccac aacacataac gttacgtacg cctatgttgt cactgggaaa
    19321 tcagcaatgg aataacctga gtattgtttc gcaaccgacg gcaaatggca ccctggttga
    19381 ggcgcaaggg cgtgaaatca acgccacgct agcgatgcgt aataacgcgc cgtggctggc
    19441 gaatatcaaa tatctttatt acaacccgag cgtggcgaaa actcgtggtg attcaacgcc
    19501 gtcatcacct ttcccgacaa cggagcgcat taacttccgt ggctggccgg acgcacaaat
    19561 acgatgcaca gagtgctggt tctgggggca aaaattcggt cgcattgaca gtgatatcac
    19621 catttctggc gatacgttaa cgctgaccaa tggactgatt gatactggtt tctcgcggct
    19681 tactgccgat ggtgaatggg ttaataatcc ggggaatgaa cgtacctcgc tgaaaggaaa
    19741 actgcgtggg cagaaaattg atgccgccgc agaatttttt ggtgtcacga cgcccatacg
    19801 ccagtcgtca tttaatgtgg attacgattt acactggcgc aaagcaccct ggcagccaga
    19861 tgaagcgacg ttgaatggca tcattcatac tcaactgggt aaaggcgaaa ttaccgaaat
    19921 caataccgga catgccgggc aattgctgcg cttattgagc gtagatgccc tgatgcgtaa
    19981 gctgcgtttt gatttcagag acacttttgg cgaagggttc tattttgact ccattcgcag
    20041 caccgcgtgg attaaagacg gcgttatgca caccgacgac acgctggtgg atggcctgga
    20101 ggcggatatc gccatgaaag ggtcggtaaa tctggtacgt cgcgacctga atatggaagc
    20161 ggttgtcgca ccagagattt ctgcgacggt gggcgtggct gcggcttttg cggttaaccc
    20221 cattgttggc gcggcagtgt ttgccgccag taaagtgctg gggccgctgt ggagcaaagt
    20281 ctccattttg cgctatcaca tttcgggtcc gctggacgat ccgcaaatca acgaagtgtt
    20341 gcgccaaccg cgtaaagaaa aagcgcaatg atttgacgag ggcgcgtaat tgccccaatc
    20401 tcatatgata atcgttgcca aaggccaacg agccggaaca taactgtagg ccggataggg
    20461 cgttcacgcc gcatccggca gccgtaaaaa atcctctact gcagtaacta acgagtagca
    20521 aaaacgatga gtctgaacct ggtaagtgaa caattgctag cggcgaacgg cctgaaacat
    20581 caggacttgt tcgcgatcct cggtcaactg gccgaacgtc gccttgatta tggcgatctc
    20641 tattttcagt cgagctatca cgaatcctgg gttttagaag accgcattat taaagatggt
    20701 tcttacaaca tcgatcaggg cgttggcgtg cgtgcaatca gcggtgaaaa aaccggattt
    20761 gcttacgctg accaaatcag cctgctggcg ctggaacaga gtgcgcaagc ggcgcgcacc
    20821 atcgtccgtg atagtggtga tggcaaagta cagacgctgg gcgcggtaga gcatagcccg
    20881 ttgtatacct cggtagatcc gctgcaaagc atgagccgtg aagagaagct ggatatcctg
    20941 cgtcgcgtcg ataaggttgc tcgcgaagcg gacaagcgcg tacaggaagt gactgccagc
    21001 ctcagtggtg tctatgaatt aattctggtt gcggccaccg acggcacgct agcggcggat
    21061 gtccgtccac tggtgcgtct ttccgtgagc gttctggtcg aagaagatgg caaacgcgaa
    21121 cgcggtgcca gtggcggcgg cggtcgtttt ggttatgagt tcttccttgc cgatcttgac
    21181 ggcgaagtcc gtgcggatgc atgggcaaaa gaagcagtgc gtatggcgct ggtcaatctt
    21241 tctgccgttg ctgcaccagc gggcaccatg ccggtagtac ttggcgcagg ttggccgggc
    21301 gtgctgttgc atgaagcggt aggtcacggt ctggaaggcg acttcaaccg ccgtggcact
    21361 tcagtattta gtggacaggt cggggagctg gtggcttcag aactgtgtac cgtggttgat
    21421 gacggcacga tggtcgatcg ccgtggttcg gtggcgattg atgacgaagg tacgccaggc
    21481 cagtacaacg tgctgattga gaacggcatt ctgaaaggct acatgcagga taaactcaac
    21541 gcgcgtttga tggggatgac gccgactggc aacggtcgcc gtgaatccta cgcccatctg
    21601 cccatgccgc gtatgaccaa cacctatatg ctgccgggta aatcgacccc gcaagaaatt
    21661 attgaatccg ttgagtacgg tatctatgca ccgaactttg gtggcggtca ggtggatatc
    21721 acctccggca aattcgtttt ctccacttca gaagcatatc tgattgaaaa cggtaaagta
    21781 acgaagccgg tgaaaggcgc aacgttgatt ggttccggta tcgaaaccat gcagcagatt
    21841 tcgatggttg gcaacgacct gaaactggat aacggcgtgg gtgtctgcgg taaagaaggg
    21901 caaagtttgc cggttggcgt gggccagcca acgttgaaag tcgataacct gactgttggc
    21961 ggtactgcgt aataatttca ttattttcag aaggataatt aatctttcta cgtgcacgaa
    22021 cggtcccctc gctcctctgg ggttagggtg aggggaaccc gttggcacag gtttgtagaa
    22081 cgtaacagta caatatgaat tacttctctt tcccgcgccc gtgcatctcc tgaaacaatt
    22141 taccgacctc aacaaaataa tccgtcagcg agttgatcac gacctgtacc ttcagcggca
    22201 gcttatcttt ttcggtatat aacgcataaa ccgggcgtgg atctgactga taacgcggca
    22261 gcaggatctc cagctcccca cgattgatct cgttgatcac ccacatcagc ggcacgtagg
    22321 cgatcccggc acccgccgtt agccagcgca ccagcgtcat cggatcatta gtcacaaatc
    22381 tcccttgcgg gatcaggcga gtcgagatcc cttccggtgc aatcagttca aattcattgt
    22441 cgggccgcac gctgtattca agccatgaat ggctacttaa atcggcgggt ttttccggta
    22501 tgccgtattg tgtgagatag cttttcgcgg cgcacaccac cattggcatc gcgccaagac
    22561 ggcgggaaaa caggctggaa tcctgcaacg cgccgacgcg gatcaccaca tccagaccgt
    22621 cggcaatcag gtcgggggct ggaattccgg taaccagatt ggcgctcaaa cctgggtatt
    22681 ctttcagcat tttggctgtc agcccggcga gaacattttg tgccatagtt gaagaacagc
    22741 caatgcgtag cgtcccgatg ggggtgttat tgaaggcata cagttgctca tgaacatcct
    22801 gcacttcatg aagcatacga cggcagccct ggtagtaaat tctaccggct tcggtcaggc
    22861 caatgctgcg tgtgctacgg tttaacagct ttacctgcaa ctcatcttcc agttttgaca
    22921 ccgtctgact gatggacgaa acgctcatct gtagctgtct ggcggcggcg gtaaaagagc
    22981 caaattcaac tactttggca aacaccgaca tgcgttttag tcgttccatt attcactctg
    23041 acttaaaagt gatttagatc acataatata gataacagca taacagttac gctaatatat
    23101 taaatatcaa tctacagcaa tgttgctctc gcccggcttt ccatgccctt ctctgcggcg
    23161 acagatgctg aaaataataa cgcctgctct ctctatacaa ccaaggtcaa catgagtctg
    23221 tttcccgtta tcgtggtgtt tgggctgtcc ttcccaccga tattttttga attgctttta
    23281 tcactggcga ttttctggct ggtgcgccgg gtacttgtgc caacaggtat ctacgacttt
    23341 gtctggcatc cggcgttgtt caacaccgcg ctctattgct gcttgtttta tttgatatcg
    23401 cgactgttcg tttgaggttg aagtgaaaac actaataaga aaattctccc gtacggccat
    23461 cacggtcgta ttagtcattc tggccttcat cgcaattttt aatgcctggg tctattacac
    23521 cgaatccccc tggacgcgtg acgcgcgctt tagcgctgac gtcgttgcga tcgcgccgga
    23581 cgtttctgga ctcattaccc aggtgaatgt tcatgataac cagctggtga aaaaaggaca
    23641 gatactgttc accatcgacc agccgcgcta tcaaaaggcg cttgaggaag cgcaagccga
    23701 tgttgcttat tatcaggtac tggcacagga gaaacgccag gaggccggac gtcgtaaccg
    23761 tctcggtgtg caggcgatgt ctcgcgaaga gatcgaccag gctaacaacg tactacaaac
    23821 ggttctgcat cagttagcga aagcgcaggc gacccgcgat ctggcaaaac tggatcttga
    23881 acgcacggtg atccgagcgc cagcagatgg ctgggtgacc aacctcaacg tctataccgg
    23941 tgagtttatt actcgaggat caacggcggt tgcgctggtg aaacagaact ccttctatgt
    24001 actggcctat atggaagaaa ctaagctgga aggggtgcgt ccggggtatc gtgcagagat
    24061 cacgccgctt ggcagtaaca aagtgctgaa agggactgtt gatagtgttg ccgctggggt
    24121 caccaacgcc agcagcacgc gtgacgacaa agggatggcg accatcgact ctaaccttga
    24181 atgggtgcgt ttggcgcaac gtgttccggt tcgtattcgt ctcgacaacc agcaagagaa
    24241 catctggcct gcgggcacca ctgctacagt ggtggtcact ggcaaacaag atcgcgacga
    24301 aagccaggat tcgttcttcc gtaaaatggc ccatcgcctg cgtgagtttg gttaatcacg
    24361 atgggtattt tctccattgc taaccaacat attcgctttg cggtaaaact ggcgaccgcc
    24421 attgtactgg cgctgtttgt tggctttcac ttccagctgg aaacgccacg ctgggcggta
    24481 ctgacagcgg cgattgttgc cgccggtccg gcctttgctg cgggaggtga accgtattct
    24541 ggcgctattc gctatcgtgg ctttttgcgc atcatcggca catttattgg ctgtattgcc
    24601 ggactggtga tcatcattgc gatgatccgc gcaccattat tgatgattct ggtgtgctgt
    24661 atctgggccg gtttttgtac ctggatatcc tcgctggtac gaatagaaaa ctcgtacgcg
    24721 tgggggctgg ccggttatac cgcgctgatc attgtgatca ccattcagcc ggaaccattg
    24781 cttacgccgc agtttgccgt cgaacgttgt agcgagatcg ttatcggtat tgtgtgtgcg
    24841 attatggcgg atttgctctt ttctccgcga tcgatcaaac aagaagtaga tcgagagctg
    24901 gaaagtttgc tggtcgcgca atatcaatta atgcaactct gtatcaagca tggcgatggt
    24961 gaagttgtcg ataaagcctg gggcgacctg gtgcgacgca ccacggcact acaaggcatg
    25021 cgcagcaacc tgaatatgga atcttcccgc tgggcgcggg ccaatcgacg tttaaaagcg
    25081 atcaatacgc tatcgctgac gctgattacc caatcctgcg aaacttatct tattcagaat
    25141 acgcgcccgg aattgatcac tgatactttc cgcgaatttt ttgacacgcc ggtagaaacc
    25201 gcgcaggacg tccacaagca gctcaaacgc ctgcggagag ttatcgcctg gaccggggaa
    25261 cgggaaacgc ctgtcaccat ttatagctgg gtcgcggcgg caacgcgtta tcagcttctc
    25321 aagcgcggcg ttatcagtaa cacaaaaatc aacgccaccg aagaagagat cctgcaaggc
    25381 gaaccggaag taaaagtaga gtcagccgaa cgtcatcatg caatggttaa cttctggcga
    25441 accacacttt cctgcattct gggcacgctt ttctggctgt ggacgggctg gacttccggc
    25501 agtggtgcaa tggtgatgat tgcggtcgtg acgtcactgg caatgcgttt gccgaatcca
    25561 cgcatggtgg cgatcgactt tatctatggg acgctggccg cgctgccgtt agggctgctc
    25621 tactttttgg tgattatccc taatacccaa cagagcatgt tgctgctgtg tattagcctg
    25681 gcagtgctgg gattcttcct cggtatagaa gtacagaaac ggcgactggg ctcgatgggg
    25741 gcactggcca gcaccataaa tattatcgtg ctggataacc cgatgacttt ccatttcagt
    25801 cagtttctcg acagcgcatt agggcaaatc gtcggctgtg tgctcgcgtt caccgttatt
    25861 ttgctggtgc gggataaatc gcgcgacagg actggacgtg tactgcttaa tcagtttgtt
    25921 tctgccgctg tttccgcgat gactaccaat gtggcacgtc gtaaagagaa ccacctcccg
    25981 gcactttatc agcagctgtt tttgctgatg aataagttcc caagggattt gccgaaattt
    26041 cgcctggcgc tgacgatgat tatcgcgcac cagcgcctgc gtgatgcgcc gatcccggtt
    26101 aacgaggatt tatcggcgtt tcaccgacaa atgcgccgca cagcagacca tgtgatatct
    26161 gcccgtagcg atgataaacg tcgtcggtac tttggccagt tgctggaaga actggaaatc
    26221 taccaggaaa agctacgcat ctggcaagcg ccaccgcagg tgacggaacc ggttcatcgg
    26281 ctggcgggga tgctccataa gtatcaacat gcgttgaccg atagttaagt caaaaccgac
    26341 gccaaaagcg tcggtttttt catggctata cttagcgatg cacggcagaa ctcgccgcga
    26401 aacgtgacgg tggcaacaga tgaatattta tgcctttgat tttgatgaga ttgagagtca
    26461 ggaggatttt tatcgtgact ttagccaaac ctttggtctg gctaaagata aggtacgcga
    26521 tctcgactca ctatgggatg tgttaatgaa cgatgtcctg ccgctaccac ttgagattga
    26581 atttgttcat ctgggagaga aaacgcgtcg ccgttttggc gcgttaatat tgctgtttga
    26641 tgaggcagag gaagagctgg aagggcattt gcgttttaat gttcgtcatt agcgcaaaaa
    26701 aaagcccccg aaccgggggc aatatcgtcg gacaagacga tgagggttta tttgtacagt
    26761 tcagccgtag cgtgccaggt gtcaccgcta cgagcttcag taatctggta ggccgttgcg
    26821 cctttctctt ccgctttttt gttcagcatt tcacgtatat ccattggcga agacgccaca
    26881 ccacttacgg atacggtccc gattgcttca cgattttgtg cttgtgcagc atcaatggag
    26941 tcggcagcga atgcaccgaa agagagaaca gaaagtacgc ttaatgcagc tacagtggtt
    27001 ttgattttca tgatttttac ctcgacataa tcttttagct gggcctttgt ttcgtgaccc
    27061 atatcacaaa atcaagtata cactaattat tgaactaatt aataccacgc taactgtttc
    27121 agttgatact aagtgttaaa aatgtgaatt ttaataacaa aaaagtataa aaaacacggt
    27181 tatttattat taattatagt attttcaatt agataatgaa ttttgcataa tgtgcttttt
    27241 ttaccgtttg tattcatttt gtgaatgatg tgtcgcggta aaacgcacta ttcgttaaat
    27301 gtatcgcagg agaaagaagg ggaatgagag ggataagcaa cattttcccc gccgccagaa
    27361 gcgacggggc agagattaaa gctcctggtc gaacagctct aaaatcgctt cgtacaggtc
    27421 tttgacggtg aaaccgttag caggggtggt aaagatggtg tcatcgccag cgatggtgcc
    27481 cagaatacct tctgctttgc ccagtgagtc cagcaggcga gcaattaact gcgccgcgcc
    27541 agggctggta tgaatcacga caactgcatc gttgtagtcg atatccagca ccagattctt
    27601 caatggactg gaggtggttg gtacacccag ttcagctggc aggcagtaaa ccatttccat
    27661 tttggcattg cgtgtacgta cagcaccaaa cttggtcaac atccgcgaga ctttagactg
    27721 attaatattg tcaaagcctt gctcctgcaa cgcggcgacg atttcgccct gggagctaaa
    27781 tttctcttct ttaagtaatg ctttaaatgc tttaactagt tcttcttgct tagccgagct
    27841 tcgcataagt cacccgatat ggtggttgat acaacattat tgtgcataca gatgaatttt
    27901 tatgcaaaca gtcagccctg aagaaggctg aaataatgtt atgaaagagc gggattttat
    27961 caaatttcgt tattgagaaa catgcctgcg tcacggcatg caaattctgt ttaaaagtaa
    28021 attaattgtt atcaaattga tgttgttttg gctgaacggt agggtatatt gtcaccacct
    28081 gttggaatgt tgcgctaatg cataagcgac tgttaattac gtaagttagg ttcctgatta
    28141 cggcaattaa atgcataaac gctaaacttg cgtgactaca cattcttgag atgtggtcat
    28201 tgtaaacggc aattttgtgg attaaggtcg cggcagcgga gcaacatatc ttagtttatc
    28261 aatataataa ggagtttagg atgaaagtcg cagtcctcgg cgctgctggc ggtattggcc
    28321 aggcgcttgc actactgtta aaaacccaac tgccttcagg ttcagaactc tctctgtatg
    28381 atatcgctcc agtgactccc ggtgtggccg tcgatctgag ccatatccct actgctgtga
    28441 aaatcaaagg tttttctggt gaagatgcga ctccggcgct ggaaggcgca gatgtcgttc
    28501 ttatctctgc aggcgtagcg cgtaaaccgg gtatggatcg ttccgacctg tttaacgtta
    28561 acgccggcat cgtgaaaaac ctggtacagc aagttgcgaa aacctgcccg aaagcgtgca
    28621 ttggtattat cactaacccg gttaacacca cagttgcaat tgctgctgaa gtgctgaaaa
    28681 aagccggtgt ttatgacaaa aacaaactgt tcggcgttac cacgctggat atcattcgtt
    28741 ccaacacctt tgttgcggaa ctgaaaggca aacagccagg cgaagttgaa gtgccggtta
    28801 ttggcggtca ctctggtgtt accattctgc cgctgctgtc acaggttcct ggcgttagtt
    28861 ttaccgagca ggaagtggct gatctgacca aacgtatcca gaacgcgggt actgaggtgg
    28921 ttgaagcgaa agccggtggc gggtctgcaa ccctgtctat gggccaggca gctgcacgtt
    28981 ttggtctgtc tctggttcgt gcactgcagg gcgaacaagg cgttgtcgaa tgtgcctacg
    29041 ttgaaggcga cggtcagtac gcccgtttct tctctcaacc gctgctgctg ggtaaaaacg
    29101 gcgtggaaga gcgtaaatct atcggtaccc tgagcgcatt tgaacagaac gcgctggaag
    29161 gtatgctgga tacgctcaag aaagatatcg ccctgggcga agagttcgtt aataagtaat
    29221 tgattagcga ataataaaaa accggagcac agactccggt ttttgttttg agcactcgac
    29281 ttaattggtt gccggatatt cctgaatggt gacctgcagc gttaactgct tatcatcacg
    29341 catcactaca accgggatca ccgaaccagg gcgaatttcc gccacctgat ccatcgtctc
    29401 cagagcagag atggccggtt tgttatccac cgaaataatc agatcgttga cctgaatacc
    29461 cgcattcgcc gctgggccgt caggtgacac ttcattaacc acgatccctt gcagttgatc
    29521 tataccaccg ctctgcgcgt gcaatggtgc gatctcgcgt ccgccgatac caatgtagcc
    29581 gcggatcacg cgaccatcgc ggatcagctt atccataatt ttggttgcta actggaaagg
    29641 aatcgcaaag ccgatacctt ccggcgtttc gccatcgtta ctcttatcaa acgacagcgt
    29701 gttaatgccc atcagttctc ccagcgagtt caccagcgcg ccgccagagt taccgtggtt
    29761 aatggaagca tcggtttgta ggaagttttg ccgcccggtc gggttcagac cgattcgacc
    29821 cgtggcacta ataatccctt gggtaatggt ctgcccgagg ttgtacgggt taccgatcgc
    29881 cagtactacg tcgccaatgt gcggtacgcg acgtgcatta attggaatgg taggtaaacc
    29941 gccagtggca ttaattttaa gtaccgccag atcggttaga gagtcagatc ccaccagcaa
    30001 tgcttcaaat acacgtccat cctgtaaggc gacgatgatc tgatcggcgt cgttgatgac
    30061 gtgtttattg gtgatgatat aaccgcgttg atccatgatt acaccggatc ccagggtgcg
    30121 gatctcaagc tggttgtgag agttggtgtt caaaccacgg ttgtaaacgt taaccaccgc
    30181 tggcgcggcg cggcgaaccg ccagattata gctggcaggc gtctcatcgg tactgtcaaa
    30241 ttgcggagtg gaaagcgggt taaggctgcg cagcgaaggc atggcaacca gcagaatagc
    30301 gccgacaatt aatccaatcg caacggaacg taagagcttc acaaacatga tggaggcgtc
    30361 attaaaaaag ggaacggcag cagcatacca cgagttaacc ggacatcaca cgtcagcctg
    30421 atgcccggtt tacgacatta acgcatcagc agatagatgc tttcattgcc gcgtacaatt
    30481 tgcagggcga tgatggccgg ttttgccgcc agcactttac gcatttcagc aatcgagttc
    30541 acccgatcgc ggttgacgcc aatgatcaca tcgtcttttt gcaagccagc ctgagcagct
    30601 gggcttcctt tgacaacttc atctatctta atacctttgc cgccatcttt tagctgacca
    30661 tcgctcaacg ttgcaccttc cagcgctggc gtgatcattt cagcgctggc cgacgaagag
    30721 gtgctggtat caagcgtcac ttctacttcc agtggtttgc cgttacgcag caggccaagc
    30781 ttcactttcg tgcccggctc tgtagtcgcg atacgagagc gcaactcagc aaagctattc
    30841 agcggtttgc cgttgaggct ggtaataata tcgcccgctt tgacgcccgc tttcgctgag
    30901 ccagaacttg gcaacacttc gctgacaaac gcgccacgct gcacgtcaag gttgaaggct
    30961 ttggcgatat cggcactcat ctcggtgcct ttgatgccta acaaaccgcg tttgatttca
    31021 ccaaagtcga taagctgctg cgccagtgtt cgcgccatat tactggggat ggcaaatcca
    31081 atcccgacgc tcccgccgcc aggcgcaagg attgcagtgt tgatgccaat taactcaccg
    31141 ttaaggttta atagtgcacc gccggagtta ccgcggttaa tggaagcatc tgtctggata
    31201 aagttttcca gaccttcaag attcaacccg ctgcggccta atgcggaaac aatgccagag
    31261 gtggcggttt gcccaaggcc aaatgggtta ccgaccgcta cggcaaaatc accgacgcgc
    31321 aatttatcgg agtcggcaat agcgatttgc gttaatttgc tcgggttttg aatttgtaac
    31381 agggcgatat cgctctggtc atcgctacca atcagttttg catcaaactc gcgcccatca
    31441 ttgagctgaa tactgatttt ctgtgcctga ttaatcacat ggttgttggt cagcacatag
    31501 cctttactgg cgttgatgat gacaccggaa cctaaacctt cgaagggttg tgcaggttga
    31561 tccggtaaat catcaccaaa aaactttttg aattcttccg ggattttctg tccctgactg
    31621 gccgttcctt ccacccgtac gctcaccact gccggaagca ctttttccag cattggagcc
    31681 agactgggga gaggggcctg atcggcaacc tggcctggaa tcgacgcgac ggcctgaaat
    31741 gacgccgaga gagttaaccc ggcacttaac gctaatgcac tcaacagctg ggtttgtttt
    31801 ttcattattc ctgctctcgt acctgaatga taagaaaaga gattcaaaac agtttgatgt
    31861 tattgaattt tcagacggct aacaatgaga caccagatta aatgatagtc ggggagggag
    31921 agaagaggag ggcgcaatgg ctgcgcccga aaaataaatt agtcgcgctt cgcgccagta
    31981 cgcagcaggc cggatgcgcc ttcagaatag tcgcgaggca tctgcaccgg tgcctgatcg
    32041 ttgctggctt cagactctgc cagacgatta cggaacgggt ttgcttcagc agacagttcc
    32101 ggcagcaggc tgctggagct ttttgccatg tgctgataca gctggcgata gtcgtgcgcc
    32161 atggtatcca gtaattccgc gctgcgggca aagtggctaa ccagctcttc gcgatactcg
    32221 tccagttcag ctttattctt ttccagttcg tactgcaacg cctgttgctg gcgtagttta
    32281 cgattaccaa aacgcatggc cacagcacca ataatgatgc cgacgactaa cccaattagc
    32341 gcatattccc aggtcatgaa catctcccgt tgtcttttgt ttccgtaggg tgttggcttc
    32401 aggctccatg cctgcggctg attatgccac tataaccgtt aattccacag aagtggaatc
    32461 ccgactgcat atcgcgtagt gtagaacggc ctttttttcg tcaaccgtga acaacggcgc
    32521 accgattatt caaggaataa caataagatc atgcaaagcg ttaccccaac atcgcaatac
    32581 ctgaaggcgc tcaatgaagg cagccatcaa cccgacgacg ttcaaaaaga ggccgtcagc
    32641 cgcctggaaa ttatttatca agaactcatc aatagcaggc caccagcccc caggacgagt
    32701 gggctaatgg cgcgggtcgg taagctgtgg ggtaaacgcg aagacacaaa gcatacgcca
    32761 gtgcgtggct tatatatgtg gggcggtgta ggacgcggga aaacctggct gatggacctt
    32821 ttctatcaaa gcctgccggg agagcggaaa cagcgcctgc actttcaccg ttttatgctg
    32881 cgggtgcacg aagagctaac tgccttacag gggcagaccg atccgctgga aattattgcc
    32941 gatcgcttta aagccgaaac tgacgtgctc tgttttgacg aattttttgt ttctgatatt
    33001 accgacgcca tgctacttgg cggtctgatg aaagccctgt tcacccgcgg tattaccctg
    33061 gtagcgacgt caaatattcc gccggacgaa ctttatcgaa atggcctgca acgtgcgcgt
    33121 tttctgcctg caatcgatgc cattaaacag cattgtgatg taatgaacgt ggacgctggt
    33181 gttgattatc gactgcgtac actcactcag gcgcatctgt ggctttcgcc actcaacgat
    33241 gaaacccggg cgcagatgga taaactatgg ttggcgctgg cgggggtgaa acgagaaaat
    33301 tcaccgacgt tagaaatcaa ccatcggcca ttggcgacaa tgggcgtcga gaaccagacg
    33361 ctggcggttt cttttactac gctgtgcgtc gacgcccgca gtcagcatga ctatattgcg
    33421 ctctcacgcc tgtttcacac agtaatgttg tttgatgtgc cagtcatgac gcggttgatg
    33481 gagagcgaag cgcggcgctt tattgcgctg gtggatgagt tttacgagcg ccatgtcaaa
    33541 ttagtggtga gtgcagaagt gccgctgtat gaaatttatc agggtgagcg gctgaagttt
    33601 gagttccagc gttgcctgtc acgtctgcaa gagatgcaaa gcgaagagta tctgaagcgc
    33661 gagcatttgg cgggttaaaa cctgccacaa atcacaaaaa ggggtcgatc tttgaccccg
    33721 acttctctat aatcctgcga ccccacgtta caagaaagtt tttttcccaa aactttttgt
    33781 gtgctggcat aggctattcg aaggggtagg tttgccggac tttgtcgtgt gaacctcaac
    33841 aattgaagac gtttgggtgt tcaccaacgt gtaactattt attgggtaag cttttaatga
    33901 aaacttttac agctaaacca gaaaccgtaa aacgcgactg gtatgttgtt gacgcgaccg
    33961 gtaaaactct gggccgtctg gctactgaac tggctcgtcg cctgcgcggt aagcacaaag
    34021 cggaatatac tccgcacgta gataccggtg attacatcat cgttctgaac gctgacaaag
    34081 ttgctgtaac cggcaacaag cgtactgaca aagtgtacta tcaccacacc ggccacatcg
    34141 gtggtatcaa acaagcgacc tttgaagaga tgattgctcg ccgtcctgag cgtgtgattg
    34201 aaatcgcggt taaaggcatg ttgccaaaag gcccgctggg tcgtgctatg ttccgtaaac
    34261 tgaaagttta cgcgggtaac gagcacaacc acgcggcaca gcaaccgcaa gttcttgaca
    34321 tctaatcggg attataggca atggctgaaa atcaatacta cggcactggt cgccgcaaaa
    34381 gttccgcagc tcgcgttttc atcaaaccgg gcaacggtaa aatcgtaatc aaccaacgtt
    34441 ctctggaaca gtacttcggt cgtgaaactg cccgcatggt agttcgtcag ccgctggaac
    34501 tggtcgacat ggttgagaaa ctggacctgt acatcaccgt taaaggtggt ggtatctctg
    34561 gtcaggctgg tgcgatccgt cacggtatca cccgcgctct gatggaatac gacgagtccc
    34621 tgcgttctga actgcgtaaa gctggcttcg ttactcgtga cgctcgtcag gttgaacgta
    34681 agaaagtcgg tctgcgtaaa gcacgtcgtc gtccgcagtt ctccaaacgt taattggctt
    34741 ctgctccggc agaaaacaat ttgcgaaaaa acccgcttcg gcgggttttt ttatggctaa
    34801 attctgaatc agcgtaaaaa atgggaagtt gctttttgct gccgtctgaa agacaggtaa
    34861 aacaaaaacc acatcgccaa taagggtcta agtcaactat ttcagactaa agcgcatctc
    34921 tttttcccca tttccggcat ccactcacca caaaggtcgc aaaatctggt aaactatcat
    34981 ccaattttct gcccaaatgt cgggtattgc tcattttttg tttgattttc gaacaaagag
    35041 agtcgttcct tattgggtaa cacaacttct gactggccac ctggccacct ggtggctggt
    35101 agcagtaaaa attctgacta tacctggagg ttttcatggc tgtcgctgcc aacaaacgtt
    35161 cggtaatgac gctgttttcc ggtcctactg acatctatag ccatcaggtc cgcattgtgc
    35221 tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga aaaggacaat ccgcctcagg
    35281 atctgattga cctcaacccg aatcagagcg ttccgaccct ggtggatcgt gagctgaccc
    35341 tgtgggaatc tcgcatcatt atggaatatc tggatgagcg tttcccgcat ccgccactga
    35401 tgcctgttta cccggtagct cgcggtgaaa gccgtctgta catgcatcgc atcgaaaaag
    35461 actggtacac gctgatgaac actatcatca acggttcagc ttctgaagca gatgccgcac
    35521 gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt cttcggtcag aagccgtact
    35581 tcctgagcga tgagttcagt ctggtcgatt gctaccttgc tccgctgctg tggcgtctgc
    35641 cgcaactggg catcgagttc agcggcccgg gtgcgaaaga gctgaaaggc tatatgaccc
    35701 gcgtctttga gcgtgactct ttccttgctt ctttaactga agcagaacgt gaaatgcgtc
    35761 tgggccggag ttaatctgta tggatttgtc acagctaaca ccacgtcgtc cctatctgct
    35821 gcgtgcattc tatgagtggt tgctggataa ccagctcacg ccgcacctgg tggtggatgt
    35881 gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt gacgggcaaa tcgtactcaa
    35941 cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtgc gctttaacgc
    36001 gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc tggctatcta
    36061 cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg atgaagatac
    36121 cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt cggttattga
    36181 tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc cgcagccacc
    36241 acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaacaggcc caggcggcct
    36301 gttttgtctt tttaatgact taa
//
LOCUS       DADLSA010000024        35876 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.024, whole genome shotgun sequence.
ACCESSION   DADLSA010000024 DADLSA010000000
VERSION     DADLSA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.024"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..246
                     /locus_tag="JN691_001223"
     CDS             <1..246
                     /locus_tag="JN691_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242716.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="JN691_001224"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="JN691_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAY7242717.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="JN691_001225"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="JN691_001225"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY7242718.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3221
                     /gene="mdfA"
                     /locus_tag="JN691_001226"
                     /pseudo
     CDS             1990..3221
                     /gene="mdfA"
                     /locus_tag="JN691_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(3262..3546)
                     /locus_tag="JN691_001227"
     CDS             complement(3262..3546)
                     /locus_tag="JN691_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242719.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3632..4447)
                     /gene="ybjI"
                     /locus_tag="JN691_001228"
     CDS             complement(3632..4447)
                     /gene="ybjI"
                     /locus_tag="JN691_001228"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAY7242720.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4447..5655)
                     /locus_tag="JN691_001229"
                     /pseudo
     CDS             complement(4447..5655)
                     /locus_tag="JN691_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            5739..6275
                     /locus_tag="JN691_001230"
     CDS             5739..6275
                     /locus_tag="JN691_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7242721.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6450..8135)
                     /locus_tag="JN691_001231"
     CDS             complement(6450..8135)
                     /locus_tag="JN691_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAY7242722.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8405..8782
                     /locus_tag="JN691_001232"
     CDS             8405..8782
                     /locus_tag="JN691_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242723.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8812..9069)
                     /gene="grxA"
                     /locus_tag="JN691_001233"
     CDS             complement(8812..9069)
                     /gene="grxA"
                     /locus_tag="JN691_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HAY7242724.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9229..9516
                     /locus_tag="JN691_001234"
     CDS             9229..9516
                     /locus_tag="JN691_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAY7242725.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9500..10222
                     /gene="nfsA"
                     /locus_tag="JN691_001235"
     CDS             9500..10222
                     /gene="nfsA"
                     /locus_tag="JN691_001235"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HAY7242726.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10283..11185
                     /gene="rimK"
                     /locus_tag="JN691_001236"
     CDS             10283..11185
                     /gene="rimK"
                     /locus_tag="JN691_001236"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAY7242727.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERWAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11273..11749
                     /locus_tag="JN691_001237"
     CDS             11273..11749
                     /locus_tag="JN691_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAY7242728.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12101..13213
                     /gene="potF"
                     /locus_tag="JN691_001238"
     CDS             12101..13213
                     /gene="potF"
                     /locus_tag="JN691_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAY7242729.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13308..14441
                     /gene="potG"
                     /locus_tag="JN691_001239"
     CDS             13308..14441
                     /gene="potG"
                     /locus_tag="JN691_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAY7242730.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14451..15404
                     /gene="potH"
                     /locus_tag="JN691_001240"
     CDS             14451..15404
                     /gene="potH"
                     /locus_tag="JN691_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAY7242731.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15401..16246
                     /gene="potI"
                     /locus_tag="JN691_001241"
     CDS             15401..16246
                     /gene="potI"
                     /locus_tag="JN691_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAY7242732.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16306..16794
                     /locus_tag="JN691_001242"
     CDS             16306..16794
                     /locus_tag="JN691_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HAY7242733.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16835..17962
                     /gene="rlmC"
                     /locus_tag="JN691_001243"
     CDS             16835..17962
                     /gene="rlmC"
                     /locus_tag="JN691_001243"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAY7242734.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18137..18868)
                     /gene="artJ"
                     /locus_tag="JN691_001244"
     CDS             complement(18137..18868)
                     /gene="artJ"
                     /locus_tag="JN691_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7242735.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19160..19828)
                     /gene="artM"
                     /locus_tag="JN691_001245"
     CDS             complement(19160..19828)
                     /gene="artM"
                     /locus_tag="JN691_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAY7242736.1"
                     /translation="MFEYLPELMKGLHTSLTLTFASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19828..20544)
                     /gene="artQ"
                     /locus_tag="JN691_001246"
     CDS             complement(19828..20544)
                     /gene="artQ"
                     /locus_tag="JN691_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAY7242737.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSYGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20551..21282)
                     /gene="artJ"
                     /locus_tag="JN691_001247"
     CDS             complement(20551..21282)
                     /gene="artJ"
                     /locus_tag="JN691_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7242738.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21300..22028)
                     /gene="artP"
                     /locus_tag="JN691_001248"
     CDS             complement(21300..22028)
                     /gene="artP"
                     /locus_tag="JN691_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAY7242739.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCSVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22246..22761)
                     /locus_tag="JN691_001249"
     CDS             complement(22246..22761)
                     /locus_tag="JN691_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7242740.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23410..25179
                     /locus_tag="JN691_001250"
     CDS             23410..25179
                     /locus_tag="JN691_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7242741.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25390..25713
                     /locus_tag="JN691_001251"
     CDS             25390..25713
                     /locus_tag="JN691_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAY7242742.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25710..26540
                     /locus_tag="JN691_001252"
                     /pseudo
     CDS             25710..26540
                     /locus_tag="JN691_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            complement(26537..27550)
                     /locus_tag="JN691_001253"
     CDS             complement(26537..27550)
                     /locus_tag="JN691_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY7242743.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27649..29079)
                     /locus_tag="JN691_001254"
     CDS             complement(27649..29079)
                     /locus_tag="JN691_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7242744.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29090..30091)
                     /gene="ltaE"
                     /locus_tag="JN691_001255"
     CDS             complement(29090..30091)
                     /gene="ltaE"
                     /locus_tag="JN691_001255"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAY7242745.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSRILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30128..31846)
                     /gene="poxB"
                     /locus_tag="JN691_001256"
     CDS             complement(30128..31846)
                     /gene="poxB"
                     /locus_tag="JN691_001256"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAY7242746.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTKFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31979..32947)
                     /gene="hcr"
                     /locus_tag="JN691_001257"
     CDS             complement(31979..32947)
                     /gene="hcr"
                     /locus_tag="JN691_001257"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAY7242747.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32959..34611)
                     /gene="hcp"
                     /locus_tag="JN691_001258"
                     /gene_synonym="priS"
     CDS             complement(32959..34611)
                     /gene="hcp"
                     /locus_tag="JN691_001258"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAY7242748.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(34755..35654)
                     /gene="lysO"
                     /locus_tag="JN691_001259"
     CDS             complement(34755..35654)
                     /gene="lysO"
                     /locus_tag="JN691_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAY7242749.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8931 a         8879 c         9221 g         8845 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtcgga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggtc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 atcctctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccacg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg gcgctggcgc tgggattcgt tagcctgcca ttgctggcgt
     2701 ggatcgccca gtcgccgatt atcatcatta ccggggagca gttgagcagc tatgaatatg
     2761 gcttgctgca agtgcctatt ttcggggcgt taattgcggg taacttgctg ttagcgcgtc
     2821 tgacctcgcg ccgcaccgta cgttcgctga ttattatggg cggctggccg attatgattg
     2881 gtctgttggt cgctgctgcg gcaacggtta tctcatcgca cgcgtattta tggatgaccg
     2941 ccgggttaag tatttatgct ttcggtattg gtctggcgaa tgcgggactg gtgcgattaa
     3001 ccctgtttgc cagcgatatg agtaaaggta cggtttctgc ggcgatggga atgctgcaaa
     3061 tgctgatctt taccgtcggt attgaaatca gcaaacatgc ctggctgaac gggggcaacg
     3121 gactgtttaa tctcttcaac cttgtcaacg gaattttgtg gctgtcgctg atggttatct
     3181 ttttaaaaga taaacagatg ggaaattctc acgaagggta aaaaaatgcc tgattgattt
     3241 gtgcgatcag gcattctcga attaatggtg atggtcgtca atctggtgtt cgataaccat
     3301 cccttcacct acgctggcaa gatggcgaac ataaggatgc gggcggtaag ccggagctgg
     3361 cgcaggagcc acatagacgg tttgcggcac agtcgcgacg ctgaccgctt gtggaacgct
     3421 gactgaatca ggaaccacaa ccactttgta tccactcggc acatcgacgg tgacactttg
     3481 cgccatcgcc acaccagtaa agcccattaa aagcgcgccc aacagtagac aatttttcat
     3541 aatcatttcg cctgaaataa tgcagcgggt taatttccgc gaattatgca gccttcattc
     3601 aggcattgtg ttgccgggcg tgctgccgtt tttattggtc aaatggcgct tcgtgtttaa
     3661 gaactttatc gatcacatcc agtacgcctt cacggttatt ggagcctgcc cggtattttg
     3721 ccgctgcgac gaccgcgctg ccggcatttt ccattgcaaa actaaagcct gcctgacgca
     3781 gcatctcaat atcgttaccg ccatcgccaa agaccaccac ttcgctgtcg tctattcccc
     3841 ataatttctg cagttggcga aggccattgg ctttatgtac gccggggata atcagatcga
     3901 tgctgccgtt gccggtgtgg actggcacca taatatcgcc gatggcctca tgtaatgctt
     3961 tttgtacttg tggaatcagt tcatcggaaa gattcaggcc aaacttaaag aaaatatctt
     4021 ctaagttgtc aaagttatcg acgtattcca gacggtgata atacatttcc gccaccgttt
     4081 tcatggcatc gtcatatttt ttgagtgtat aggcgctatt ttttccgcag gcaataattt
     4141 ccacttccgg gcgcgtcagc aaatgttcca cgacagtagc aaacgcatcc ttcgacagct
     4201 cgccattaaa aacatctttg ccttcactca ctacccagcc gccgttttca gccacaaagg
     4261 caatttcatt cgcaatttca gggaagaaag agatcaactg ataatattga ttcccgctgg
     4321 cgaccacaaa gcgaatgcct tgtgctttca tttgctgata ctgagccata aaccgctcac
     4381 ggttataggt tttttgatcg cttaagaaag taccatccat gtctaccgca attaatttaa
     4441 tgctcatcaa ctattctcca tcgccgtctg cgttttggta tcgggtttgg cgacggcttt
     4501 cgcgacaata gccgcgagaa taaccagcgc cagtacaacc agcattgcac tacgtaatcc
     4561 ataatgttcg ccgagatagc ccagcagcgg cggcccgacg aggaaagcca gataaccggt
     4621 cgttgctacc acactgacgc gggttggtgc atcggggccg gtatcgctgg cggcagaaat
     4681 ggtcagcggg aagcccagcg aggcacccag tccccaaagt acaacagaca ctcctgcgac
     4741 ccaggcgcta tcgacaaaaa taatcagccc aatacccaac gcccccatta gtgcactggc
     4801 ccgaaccacg gcaacgcgac tgtaacggtc gatgaaccaa ccgccggtga agcgtccaac
     4861 ggtcatccct agggtaaaac cggcataaat cagtgagccg gaagtggggc taaaaccgtg
     4921 accatcaacc attaataagg gtaactagtc gttggcagaa ccttcggcaa aggccatcgc
     4981 cagcactaca acaccaatca acagcaactg gatatcgcga taaaaaggaa tgcctttttc
     5041 gccatgctgg gtgccatcgg cagcattttt gcccgtaccg tcagggattg cctgaatggc
     5101 gatataaata ggcgcgatac ctaccagcgc cgccaataaa atgtgcaccg ttgccggaac
     5161 gccaaaggcc gtcagtgcca tcccgacacc agcgcctgcc agcgtgccca ggctataaaa
     5221 accgtgcatc atcggcaaaa ccgttttatt catttctcgc tcaacggcgg caccttcaac
     5281 gtttatcgcc acttccgcag aaccaaaact tgccccaaag acgccgagac caacggcaaa
     5341 gagcaggggc gatgtcagcc agagtgccag acttaatatc atcatcccga tcaatgcgca
     5401 ggacatcgtg accaggatga cattacgtgt cccaaagcgt ttcactaacc acgccgagca
     5461 gagaataccg ctcatcgaac cgatcgacag accaaagaga acaccgccca tttcagcgat
     5521 cgagacagag agaatatcgc ggatagcagg cgtacgggtt gcccaggacg ccattaacag
     5581 gcctggcaaa aagaagaaca taaacagcgc ccaggttcgg cgtttcaatg cattacgtga
     5641 agaatttacg gtcatagatc acgtcaaaat aagaagaggg aagacaacat tagcaaggtt
     5701 gtgtacattt gtacacaatt gcagacagag gaaatgacat gcgtcgcgct aacgatccgc
     5761 aacggcgaga aaaaattatc caggccacgc tggaggcggt gaaactttac ggaatacatg
     5821 ctgttacgca ccgcaaaatt gctacccttg ccggggtacc gttgggatcg atgacctact
     5881 atttttcagg aattgatgag ttgttgctgg aggcgttcag cagttttact gagattatgt
     5941 cccggcaata tcaggcattt tttagcgatg ttagtgatgc tcaaggcgca tgccaggcta
     6001 tcaccgatat gatctacagc tcacaggttg caacgccgga taatatggag ctgatgtacc
     6061 agctctacgc gctggctagc cgaaaaccgc tattaaaaac ggtaatgcaa aactggatgc
     6121 agcgcagtca gcaaacgctc gaacaatggt ttgaacccgg aaccgcccgc gcgcttgatg
     6181 cgtttattga ggggatgacg ctgcattttg tcaccgaccg taagccgcta tcgcgcgagg
     6241 agattttgag gatggttgag agggttgcag ggtagtagat aaattttaga taacaaaaaa
     6301 cccatcaacc ttgaaccgaa atggcggggt tgatgggctc cacaaaatgg ggacatcaaa
     6361 gaaaagcagt ggcactaatt aagactgatg ccctgcggaa aagttctgcg gttgtgcaaa
     6421 aaaatttcat tttcagggca acttcagttt tatcctaatc ctggccatac catgacgatg
     6481 attgtccctg ccagcgtcag caggacgttg gcgattgcgt aggtgcccgc atagcccagc
     6541 gcagggatgt tactgcgagc tgtatcactg atgatctcca ttgccggcgc gcaggtgcgt
     6601 gcgcccatca ttgcgccgaa caacagtgcg cggttcattc gcaatacata agcaccgaac
     6661 aagaaacaga taaccacggg cacaagactg acaattaatc cggcaatcaa catctgaccg
     6721 ccaatcgcgc ccaggccgtt attaataccg ctaccggcgc tcagaccaac gcctgccata
     6781 aacaccatca agccgaactc tttcaccatg cttaatgcac cttgcggaat gtaaccgaag
     6841 gtcgggtggt tagcacgcat aaagcccagc ataattccgg cgaataacaa cccggcagcg
     6901 ttccccatgc cgaaactgaa tgtgctgaac tggaaggtga tcatcccgat catcagccca
     6961 ataacaaaga aggcgcaaaa tgccagcagg tcagtgacct ggctgtgaat cgagataaag
     7021 ccgatgcgat cggcgatggt ttttacgcga cgggcatcac cgctgacttg taaaacgtca
     7081 cctttgttaa gtacgacgtt gtcatctatc ggcatctcaa tctggctacg aatgacgcgg
     7141 ttaaggaagc aaccgtgatc ggtcaacttc agttgtgcga gacgtttacc tacagcgtta
     7201 tggtttttaa cgaccacttc ttcagtgacg atacgcatgt cgagaaggtc acgatcgaaa
     7261 acttctttac cgttacggaa gctgggatcg agtcgggcat gggcgtcggg atagcctacc
     7321 aacgctattt catcgcccat ttgtagcacg gcatcaccgt ctggatttgc cagaatcccg
     7381 ttacgtcgaa tacgttcaat gtagcagccg gtttgtcgat aaatacccag ttcacgcaga
     7441 tttttgccgt cggtccaggc caccagctcc gggccgacgc gataggcgcg gatcaccggt
     7501 aaataaacct tacggttggc atcagtgtcc aggccacgtt cgcgggcgat ttgctgggcg
     7561 ctggtctgta agtcctgatg ctgcaatttc ggcaagtaac gcgcaccaac aatcaaactc
     7621 accagaccga ttaaataggt taaggcatac ccgaggctca gattatccag tgccagtgag
     7681 agctgcctgc tttccatgcc ggaatgacgc agtgtatcgc cagcaccgac cagaaccggt
     7741 gtcgacgtca tagagcctgc taacataccg gccgtcaggc caatatccca gccaaacagc
     7801 ttacctaacc ctaaggcgat caccagcgca ctgccaacca tcaccagtgc taacattagg
     7861 taatttttcc catcgcgaaa aaaaatggaa aaaaagttcg gtccggcttc gaccccgacg
     7921 cagaaaataa acagcataaa gccaagatta agcgcatcgg tgttaatgct gaaatgttgt
     7981 tggcctaata acagcgatac gactaaaacg ccaatggaat tacccagttg gatcgaacca
     8041 agtcgtaact ttccgagaca tagcccaagc gcgaggacca caaataataa cagaatgtaa
     8101 ttcccattta acaattcggc gacgtttata ttcacggagg ctaacttctt gtttactagt
     8161 aagctgttga aagaaatggt aatttacgat aatgtttttt accagaattc agggcgcaga
     8221 ttcattcagc gcacctaaac gatagtaaag taacaatata ttttactagt gtaatcacat
     8281 taggtatcaa cggctatatg aattgcgttg gcctatatta gcatggaatg cgaagcggct
     8341 ttatcttact gaacgccaca ctggcgaaaa atgtgttcga tagacgcagt gtcaggagga
     8401 acgagtgaaa cataaacaac gttgggcggg ggcaatctgc tgttttgtcc tcttcattgt
     8461 ggtgtgcctt tttctggcga cgcacatgaa aggcgctttt cgggctgccg ggcatcctga
     8521 aatcggcttg ctgtttttca ttcttcctgg agcagttgcc agtttctttt cacagcgtag
     8581 agaagtcctg aaacctctat ttggcgcaat gctggcggca ccctgttcaa tgctcattat
     8641 gcggctgttt ttttcaccga cgcgctcatt ctggcaagag ctggcatggt tactaagcgc
     8701 ggtgttctgg tgtgcgctgg gggcactgtg tttcttattt atcagtagtt tgtttaaacc
     8761 acagcacaga aaaaatcagt aaagccctca acgcgagggc ttgtcagacg atcaggcgtc
     8821 cagattttct ttcacccatg cagcaaaatc ggtatagccg ccgatatgtt gctgatcgac
     8881 aaaaatctgc ggcacggttt ctacgggttt acctgccttt tgttgtagat cttctttagt
     8941 gatcccttcc gcacgaatat ctacatactg atactgaaaa tcatcgcgtt cattgctcaa
     9001 tttctcagcc agatcttttg cacgcacaca gtaagggcaa cccgaacgac caaaaataac
     9061 ggtttgcatt atttctctcc tcatagattt atgcctgtaa tgatcacgct aaaatgtatt
     9121 cgctgaaagt aggtttaacc tgttgcatta attgctaaaa gctataactg ttaaacacaa
     9181 tacagtgaaa agttttagac tgaaggctca ctttgcagag ggaagcgtat gcgcgcgatc
     9241 ggtaaattgc ctaaaggcgt gttgatactg gaatttatcg gaatgatgct actggcggtg
     9301 gcgctgctgt cggtaagcga ctccctgtcg ctgcctgagc cattttctcg gccagaagtg
     9361 cagattctga tgatttttct cggtgttttg ctcatgcttc ccgctgcggt ggtggttatt
     9421 cttcaggtgg caaaacgtct tgccccacag ctgatgaacc gtccaccgca atattcacgt
     9481 tcagaaagag aaaaagataa tgacgccaac cattgaactt atttgtggcc atcgctccat
     9541 tcgccatttc actgatgaac ccatttccga agcgcagtgt gaggcgatta ttaacagcgc
     9601 ccgtgcgacg tccagttcca gttttttgca gtgtagtagc attattcgca ttaccgacaa
     9661 agcattacgt gaagaactgg tgacgctgac cggcgggcaa aaacacgtag cgcaagcggc
     9721 ggagttctgg gtgttctgtg ccgactttaa ccgccattta cagatctgtc cggatgctca
     9781 gctcggcctg gcggaacaac tgttgctcgg tgtcgttgat acggcaatga tggcgcagaa
     9841 tgcattaacc gcagcggaat cgctgggatt gggcggggta tatatcggcg gcctgcgcaa
     9901 taatattgaa gcggtgacga aactgctgaa attaccgcag catgttctgc cgctgtttgg
     9961 gctgtgcctt ggctggcctg cggataatcc ggatctcaag ccgcgtttac cggcctccat
    10021 tttggtgcat gaaaacagct atcaaccgct ggataaagac gcactggcgc agtatgacga
    10081 gcaactggcg gaatattacc tcacccgtgg cagcaataat cgccgggata cctggagcga
    10141 tcatatccgc cgaacaatca ttaaagaaag ccgcccattt attctggatt atttgcacaa
    10201 acagggttgg gcgacgcgct aaaaccgcca cgtcgatgta tgatacgcgg gcttttgacc
    10261 aggtctgaca gagaggtgca gggtgaaaat tgccatattg tcccgggatg gaacgctcta
    10321 ttcgtgtaag cggctgcgtg aagccgctat acagcgcggt cacctggttg aaattcttga
    10381 tccgctttct tgctacatga acataaatcc tgcggcgtct tctattcact acaaaggccg
    10441 caagttaccc cattttgacg cagtgatccc gcgtattggc accgccatta ccttttatgg
    10501 gacggcggca ctgcgccagt tcgagatgct ggggagctat ccgctcaatg agtcggtcgc
    10561 cattgcccgg gcgcgtgaca aattgcgttc catgcaactg ctggcgcgtc agggcatcga
    10621 cctgcctgtc acgggcattg cgcattcgcc ggatgatacc agcgatttaa tcgacatggt
    10681 cggtggtgcg ccgctggtgg tcaagttggt tgaaggcacg cagggaattg gcgtcgtgct
    10741 ggcggagact cgtcaggcgg cggaaagcgt gattgacgct ttccgcggtc tgaacgcgca
    10801 tattctggtg caggaatata tcaaagaggc gcaagggtgc gatatccgct gtctggttgt
    10861 tggtgatgaa gtggtcgctg cgattgaacg gtgggcgaaa gagggctatt ttcgttccaa
    10921 tttgcatcgt ggcggcgcgg caagcgtcgc cagtatcaca ccacaggagc gtgaaatcgc
    10981 gataaaagcc gcgcgaacga tggcgctgga cgttgctggt gtggatattc tgcgtgctaa
    11041 tcgcgggccg ttggtgatgg aagtgaatgc gtcgccgggg ctggaaggaa tagaaaaaac
    11101 caccggtatc gatatcgcgg gtaaaatgat ccgctggatc gaacgccacg ctacgacaga
    11161 atattgcctg aaaacgggtg gttagtcgca atcacattac tgatcatggt tttgcctgcg
    11221 ctttttgcgt aagctgtgcc ggtcttttta tcgaaagagg ttgtacaaaa ttatgacatc
    11281 gctggtcgtt cctggtctgg atacgctgcg tcaatggctc gatgacctgg ggatgagttt
    11341 ttttgaatgt gataactgtc aggctctgca tctgccccat atgcagaatt tcgacggtgt
    11401 ctttgatgcc aaaatcgatc tgatcgataa cacgatcctg ttttctgcca tggcggaagt
    11461 ccgaccttca gccgtattgc cgctggcggc ggatttatct gccatcaatg ccagttcgct
    11521 gaccgtgaaa gcatttcttg atatgcagga tgataatctg ccaaagctgg tggtttgcca
    11581 gtctttatcc gttatgcagg gcgtaaccta tgagcagttt gcatggttcg tgcgtcagag
    11641 cgaagagcag atttcgatgg tcattcttga agctaatgcc catcaactgc tgttaccgac
    11701 tgacgatgaa gggcaaaaca acgttaccga aaactatttt ctccactgat aattcctttc
    11761 gagcacgcag tcgctgccgc agtgactgcg tgctgcaaaa ttatctgctg tttttaacct
    11821 tttcttaaag attatttcac ttctcttgtg tcgatttggc tttatcacat agagcaaata
    11881 cgcataaaaa tttgttaaat accgtttttt aatccgagct atagtctcaa accctggcta
    11941 aagttattct tgcgatactt ttatatagcg agcagtgctg gccgggagag agttctcttt
    12001 tcttacaccg cgccgataaa aaaatatgca cgtttattgc atatctttca gtgtgacaac
    12061 ttttgttcgt ttgttaacga actttcagaa ggaaagagat gtgaccgcct taaataaaaa
    12121 atggctatcg ggtctggttg cgggtgctct gatggccgtc tctgtcggca cgctcgcggc
    12181 tgaacaaaaa acactccaca tttataactg gtctgattat atcgcccctg acacggtggc
    12241 caattttgaa aaagaaaccg gtattaaagt cgtctacgat gttttcgact ctaacgaagt
    12301 actggaaggc aaattaatgg cgggtagcac cggctttgac ttggtggttc catctgccag
    12361 ctttcttgaa cgccagttga ctgcgggagt tttccagccg ctggacaaaa gcaaattgcc
    12421 ggagtggaag aatctcgacc cggagctgct gaagctggtc gccaaacacg atcccgacaa
    12481 taaatttgct atgccctata tgtgggcgac gaccgggatt ggctataacg ttgataaagt
    12541 taaagctgtg ctgggcgaaa acgcgcccgt cgatagctgg gacttgatcc tcaaacctga
    12601 aaatctggaa aaactgaaaa gctgcggtgt ctctttcctg gatgcgccag aagaagtttt
    12661 tgctaccgtg ttgaattatc tcggcaaaga tcccaacagc actaaagcgg atgattacac
    12721 cggaccggca accgatctgc tgttaaagct gcgcccgaac attcgttatt tccattcatc
    12781 tcaatacatt aacgacctgg caaacggcga tatttgcgtc gctatcggct gggcaggtga
    12841 tgtctggcag gcgtcaaacc gcgcgaagga agcgaagaat ggagtgaatg tctcgttctc
    12901 gattccaaaa gaaggggcga tggcgttctt tgatgtattc gccatgcctg cggatgccaa
    12961 aaacaaagac gaagcctatc agttcctgaa ttacctgctg cgcccggatg tagtagcgca
    13021 tatttccgac catgtgttct atgccaacgc caataaagca gccacgccgc tggtgagtgc
    13081 ggaagtccgt gagaacccgg gtatttatcc gcctgcggat gttcgtgcga agctgttcac
    13141 tctgaaagtg caggacccga aaatcgaccg tgtgcgcacc cgcgcgtgga ccaaagtgaa
    13201 gagcggaaaa taatccgcag ccttagatgc cgggcgggcg caccacaccc gccggcaatt
    13261 cgcaccatca tggtgcgctt gcacacattc aatgccggag agcagccgtg aatgacgcta
    13321 tccctcgccc gcaggcgaaa acccgtaagg cgctgacgcc gctattagaa atccgcaacc
    13381 tcaccaaatc ctacgatggt caacatgcgg tggatgatgt cagcctgacc atctacaaag
    13441 gtgaaatctt cgcgctgctg ggcgcatccg gctgtggcaa gtccacgctg ctgcgtatgc
    13501 tggcaggttt cgaacaacct tctgccggac agataatgct tgatggcgtc gatttgtcac
    13561 aggttccgcc ttacctgcgc cccatcaata tgatgtttca gtcttacgcg ctgtttccgc
    13621 atatgaccgt ggaacagaac atcgcttttg gcctgaaaca ggacaaacta ccgaaagcgg
    13681 aaatttccag tcgggtcaat gagatgctcg gcctggtgca catgcaggag ttcgccaaac
    13741 gtaaaccgca tcagctttct ggtggtcagc gacagcgtgt ggccctggct cgaagccttg
    13801 ccaagcgccc gaaactatta ctgctcgacg agccgatggg cgcgctggat aaaaagctgc
    13861 gtgaccggat gcagcttgaa gtggtggata ttctggagcg cgtcggtgtg acttgtgtga
    13921 tggtcaccca cgatcaggaa gaggcgatga ccatggcggg gcgcatcgcc attatgaatc
    13981 gtgggaaatt tgtccagatt ggcgaaccgg aagagatcta cgagcatccg actacccgct
    14041 atagcgccga atttattggc tcggtaaacg tctttgaagg cgtactcaaa gagcgtcagg
    14101 aagatggcct ggtgcttgat tcgccggggc tggtgcatcc actgaaagtc gacgcggatg
    14161 cctcggtggt cgataacgtg ccggtacatg tggcgctgcg cccggaaaaa atcatgcttt
    14221 gcgaagagcc gcccgccaat ggttgtaact tcgcggtggg ggaggtgata cacattgcct
    14281 atctcggcga tctttcggtg tatcacgttc gtctgaaaag tgggcagatg attagcgccc
    14341 agctacaaaa cgcccatcgt catcgtaaag ggttaccgac ctggggcgac gaagtgcgtt
    14401 tgtgctggga agtggacagc tgtgtggtgc tgacggttta aggagcaaag atgaatacac
    14461 ttgaacctgc tgcccagtcg aaaccgccgg gcggatttaa gctgtggttg tcgcagctgc
    14521 aaatgaagca tgggcgcaaa ctggtcattg cgttgccata tatctggttg atcttgctgt
    14581 ttctgctgcc atttctgatt gtctttaaaa taagcctggc ggagatggcg cgcgctattc
    14641 caccttatac cgaactgatg gagtgggctg acgggcaact gtccatcact cttaatctcg
    14701 gtaattttct gcaactgacc gacgatccgc tctatttcga tgcttatctc cagtcgctac
    14761 aggtggctgc gatttcgaca ttttgctgtt tactgatcgg ctatccgctg gcgtgggcgg
    14821 tggcgcacag taagccttcg acccgtaata ttttattact actggtgatc ctgccgtcgt
    14881 ggacctcgtt tctgatccgc gtttatgcct ggatgggaat attaaaaaac aacggtgtgc
    14941 tgaataattt tctgctgtgg ctgggggtta tcgatcaacc gctgaccatt cttcatacca
    15001 atctggcagt ttatattggc attgtttacg cctacgtgcc gtttatggtg ctgccgattt
    15061 ataccgcgtt gattcgtatt gattattcgc tggtggaagc agcgctggat ctcggtgcac
    15121 gaccgctgaa aacgttcttt actgtgatcg tgccgctgac taaaggtggg attattgccg
    15181 gatcgatgct ggtgtttatc ccggctgtgg gcgagtttgt gatcccggaa ctgctcggtg
    15241 gcccggacag catcatgatc gggcgcgtgc tatggcaaga gtttttcaat aaccgcgact
    15301 ggccggtggc ctcggcggta gcgatcatca tgttgctgct gctaattgtg ccgataatgt
    15361 ggttccacaa acaccagcaa aaaagcgtgg gagaacacgg atgaataatt taccggtagt
    15421 tcgttcgccc tggcggattg tgattttgct gctgggcttt acctttctct acgcgccaat
    15481 gctgatgctg gttatctatt cgtttaacag ctcgaagctg gtgacggtgt gggccggctg
    15541 gtcaacgcgc tggtatggtg agttattgcg cgatgacgcg atgatgagtg cggttggttt
    15601 aagcctgaca attgctgcct gtgcggcaac ggcggcggcg atcctcggga ctattgcggc
    15661 ggtggtgctg gtgcgctttg gcaggtttcg cggatcaaat ggctttgcct ttatgatcac
    15721 cgcgccgctg gtaatgccag atgtcatcac gggcttgtcg ctgttgttat tattcgtcgc
    15781 gcttgctcat gccattggct ggcctgcgga ccgcggtatg cttaccatct ggctggcgca
    15841 tgtcactttc tgtacggctt atgtggcggt cgttatttcg tcgcgtctgc gggaactgga
    15901 tcgctcgata gaagaagccg cgatggatct cggtgcgacg ccgctgaaag tgtttttcgt
    15961 cattacgcta ccgatgatca tgcccgcgat catttctggc tggttactgg cttttacttt
    16021 gtcgcttgat gatctggtga tcgccagctt tgtttccggg ccgggagcca ccacgttacc
    16081 gatgctggtc ttttccagcg tgcggatggg ggtgaatccg gaaatcaacg ccttggcaac
    16141 attgatcctc ggcgcggtcg gaattgtcgg atttatcgcc tggtatctga tggcgcgcgc
    16201 agaaaaacag cggatacgcg acatccaacg tgcaagacgt ggctgaagac actaaaattt
    16261 gccaacctgg ctacataatg ccgcgcatgt cgcggcattg ttttcatgga agacgaaacg
    16321 ttgggatttt ttaagaaaac atcttcatct catgctcgcc tgaatgtgcc tgcgctggtg
    16381 caggtggcgg cgctcgccat tattatgatc cgtggcctcg acgtgctgat gattttcaat
    16441 acgctgggcg tgcgcggtat tggcgagttc attcatcgca gcgtacaaac ctggagttta
    16501 acgctggtct ttttaagcag tctggtgctg gttttcattg agatctggtg tgcgttttca
    16561 ctggtgaaag ggcgtcgctg ggcgcgctgg ctatatctgc tgacacaaat caccgccgca
    16621 agttacttgt gggcggcttc gctggggtac ggttatccgg agctgttcag cattcccggt
    16681 gaatcaaaac gtgaaatctt ccatagcctg atgctgcaga agctgccgga tatgctcatc
    16741 ctgatgctgc tgttcgttcc ctcgaccagt cggcggttct tccagttgca ataatgtgta
    16801 taatcgtcgc ccctgatgat gtgaaggtca atgtatgcag tgcgcacttt acgacgcggg
    16861 tcgctgtcgt tcctgtcagt ggataacgca gccgattcca gagcaactct ccgctaaaac
    16921 cgccgatctt aaaaatctgc tcgccgattt tccggttgag gaatggtgcg cgccggtgtc
    16981 aggcccggaa caagggtttc gtaataaagc caaaatggtg gtgagtggta gcgttgaaaa
    17041 accactgctc ggtatgctgc atcgcgatgg cacgccggaa gacctttgtg actgcccgct
    17101 ttatccagcc tcatttgcgc ccgtttttgc ggcgctaaaa ccgtttatcg cccgagcggg
    17161 gttaacgccc tacaacgtgg cgcgtaagcg tggcgaactg aaatacattc tgctgactga
    17221 aagccagagt gatggcggca tgatgctgcg ctttgtactg cgttctgata ccaagctggc
    17281 gcaactgcgt aaggcgctgc cgtggttaca cgaacaacta ccgcagctga aagttattac
    17341 cgtcaatatt cagccggtac atatggcgat tatggaaggg gagacggaga tctacctgac
    17401 cgaacaacag gcactggcgg agcgttttaa tgacgtaccg ctgtggatcc gtccgcaaag
    17461 tttcttccag actaatccgg cggtcgccag ccagttgtac gccaccgcgc gcgactgggt
    17521 acgacagctg ccggttaaac atatgtggga tctgttctgc ggtgtggggg gctttggttt
    17581 acactgcgcg acgcctgaca tgcagttaac cgggatcgaa attgcgccag aggccattgc
    17641 ctgtgcaaag cagtcagccg ctgaactggg cttaactcgt ttacaatttc aggcgctgga
    17701 ctccactcag tttgccactg ctcaggggga ggtgccggag ctggtgctgg ttaacccgcc
    17761 gcgccgcggc attggtaaac cgctgtgtga ttatctttca acgatggcac cgcgttttat
    17821 tatctactcc agctgtaacg cccaaaccat ggcgaaagat atccgcgaac tgccaggtta
    17881 ccgcatcgaa cgggtacagc tttttgatat gttcccgcac accgcgcact atgaagtgct
    17941 gacgctgctg gtgaagcaat aaaaaagcca caggtgcggc ttcagattgc tgatcaggtg
    18001 cgctttgttc atgccggatg cggcgtgaac gccttatccg gcctacaaaa tcatgctaaa
    18061 tcaatacatt gcagagttga tgtgggccgg ataagcgcag cgcatcaggc aatcttactt
    18121 ttgtcatcag tctcaattac tgcggaaacc actggtcatt gattttctga tatgtaccgt
    18181 cagctttaat tgctgccagc gcgttattca gtttttccag cagagctttg ttatccggac
    18241 gtacagcgat acccaggccg gtgccgaagt attgcggatc ggtcactttt tcagtcgcaa
    18301 cgcccagttg cggattggtt ttcagccatt cgtttaccac cgcggtgtca ccaaataccc
    18361 catcaatacg accatttttc agatcgataa aggcattctg ataactgtca taggagacag
    18421 ttttcacttc cgggtgctga tcctgaatat atttctggtg cgtcgtaccg ttttccatcc
    18481 caatgcgttt gcctttcagg tcggcaaacg ttttgtaggt atcttttttg gcaatcacga
    18541 cggctgagtt ttcatagtag ggtgtggtaa acgaaacctg cttgctacgc tccggggtga
    18601 tatccatacc ggagattacg gcgtcatatt ttctgaattt cagtgacggg atcaggctgt
    18661 cgaacgcgtg attagtaaaa gtacattctg cctgcatttg tttgcacaag gcttttgcca
    18721 gatcgatatc aaagccgaca atctcattat tagcacctat agattcaaag ggtggatagg
    18781 tggctgaaac gccaaaattg attttctctg cggcagaagc accgaaagta aaggaagcaa
    18841 gtaaagcggc aagaactaac tttttcatga tggaactccc gtctgtcaat cttatgattt
    18901 ttggccgtgt ctgcggcatg ggataacaat gccaccaagt gaatttatat gcaataaaca
    18961 tgattaaata atttaaatga aataaaaaaa gacggataac ttagtgagtt gtccgtcttc
    19021 attataagaa tttatgcact atgtaggccg gataaggcgt ccacgccgca tccggcacag
    19081 gcaccgtgct gatgtctgat gcgacgctgg cgcgtcttat cagacctaca aacccccccg
    19141 gcgaatgtac gcagctacat taattccgcc gttcgaatgc cagtgctttg cgctcgatca
    19201 gacgcatcat cagcgtcagc aggccgttga cgaccaggta aataatccct gccgcaccga
    19261 acaccattac atcgtaggtg cgtccgtaca acaactggct gtatcccatc acttccatca
    19321 gcgtaatggt gtaagccaga gaggtacttt tgaacaccag caccacttcg ttggaataag
    19381 aagagagcga gcgtttaaag gcatacggca gcaggatcgc cagcgtatct tttttgctca
    19441 ttcccagggc gctacaggac tgccactgac cttctgggat cgcacgaatt gcaccgtaaa
    19501 acagctgcgt ggtatacgcc gcactgttca gcgataacgc aatcagcgca cataaccacg
    19561 gttctgacaa caaatgccac agtgccggat actcctgcaa cgtcggaaac tggcccggcc
    19621 cgtaataaat caggaagatc tgcaccagca gcggcgtacc ggtaaacagc gtgatataac
    19681 cccgcaccag ccacaccagc accggcgttt tcagcgtcag gatgatggta aaaatcaatg
    19741 ccagaatcag tgccacaatc agcgaggcaa aggttagcgt caggctggtg tgcagccctt
    19801 tcatcagctc gggtaaatac tcaaacatta gctgggcctc cgctcaaaac gtgtcgcgcg
    19861 caggtcaatg cgtttgagaa tgtactgact gagcagggtg atcaccaggt aaatcgccgc
    19921 cgccacaatg taccaggtaa atggttcctg ggtacgagta gcaatgcttt tggtttgtaa
    19981 cattaaatca ttcacactaa tcaaactgac cagcgcggta tctttcagca gcaccagcca
    20041 ctggttaccg aggccaggca gcgcatgacg ccacatctgc ggcatcacca gacggaaaaa
    20101 gatagccgat ttcgacagcc ccagcgcctg accagattcc cactggccca ccggtacagc
    20161 tttcaacgcg ccgcgcagcg tttgcgaggc ataggcggca tacagcagtg acagagcgat
    20221 gacaccacaa aggaacggac tcacgtcgaa gttctcaatg tccatctgca ccgggatctg
    20281 cacgaaccca agattgatag tgaagccata cgaaagcgtc agcagtaact gcgaggagcc
    20341 aaaatagata aacagcacta ccagaatttc tggcaggcca cgcagaatgg ttaccagcgc
    20401 tgaacctgcc cacgcgacag gacgccattt cgctgactcc cataccgcaa agaacatcgc
    20461 cagcgccagc ccgacaatca atgcacaaac ggcaaggccg acggtcatcc cggcggcgct
    20521 tgctaaagga aaaaattcat tcatcaggaa ttacttctgg aaccatttgt tgtagatggt
    20581 ttcgtaagtg ccatctttct tcactttttc cagcgcagtg ttgagtttct gctgcagttc
    20641 agtgttgccc tgacgtaccg cgatgccgag gccagtaccg aaataatctt tatcggtcac
    20701 tttatcgccc actgccgcca gttttgggtt atctttcagc cactcagtga ccactgcggt
    20761 gtcaccaaat acgctgtcga tacgcccgtt ttgcagatcc agttttgcgt tctggtagct
    20821 gtcatacgga acggtggtga tttccgggtg cttatccata atgaacttct ggtgtgtcgt
    20881 cccgttctga acgccgactt ttttgccttt cagctgatca acactggtgt atttgccttg
    20941 ctgacccaca aacagggcag agttgtcata gtacggggtg gtaaacagta cctgcttttc
    21001 acgctccgga gtgatatcca tgcccgccat cactgcttct acgcgacgga atttcaggct
    21061 tgggatcagg ctgtcaaacg cctggttaga gaaagtacag gttgcatcaa tctctttaca
    21121 cagtgcctgt gccaggtcga cgtcaaaacc aacgatctgg ttgtttgcat caatcgattc
    21181 aaacggagga taggaggctt cggtagcaaa acgaatggtt tcggcagctg tggcggaaag
    21241 actaaaacct gcaattaacg cggcaatcag aacttttttc attgttgtta tcccgaatct
    21301 tagtgagaga gatagttttt aaatgcttcg gtttgcggct cggtaaagca gctcgcgtcg
    21361 ccttgttcta cgatatgacc attttccata tacaccactc ggctggcggt tttacgcgcc
    21421 acttcaactt cgtgggtgac gatcacctgg gtaatattcg tttctgccag ctcacgaatg
    21481 atgctgacga tttgtgccgt aatttccggg tccagtgcgg cggtcggttc atcgaacagc
    21541 agtacctgcg gttccatcat caacgcacgg gcaatagcaa cacgctgctg ctgaccacca
    21601 gaaagatgca gcgggtaacg atcgctataa ggtttgagac gcagacgttc cagcagtttt
    21661 tctgcacggg ccagcgcctg atctttactc aaccccagta cactgcaggg cgcttcaatc
    21721 aggttttgct gcacggtcag atgcggccac aggttgtatt gctgaaacac catgccaacg
    21781 ttacgacgca aatcgcgaat cgctttgtca gagggtgttt tggtgaaatc gaaatggttg
    21841 cctgcaatgt tgagcgtacc ggagcgcggc atctcaagca gattgagtac acgcagcagc
    21901 gagcttttac ccgcgccgct ggggccaagt aacaccagcg tttcgccctg tgggcaatcc
    21961 agcgtgatat cgaacagcgc ctgatgcgcg ccgtagaagc aattaatgcc gtttaattga
    22021 atactcattg acactcgtat actggcagtc tgatagctat tgaggtcgaa gatagtacct
    22081 ttgacagaat aattatgcaa tatttatgct ttaaaagtta aaagcaaagc gcattattca
    22141 ataaacatag cacaaaataa cgggggcggt ggtcggtgag cataaatgtc ggcattcctc
    22201 acgaaatgcc ggataattta cggggtttat tggttgatca aggcgttagc gattctcgat
    22261 ggactgacgg agcgtacccg ccgtggcatg aacgctaccg cctaagtagc gcacatcgtc
    22321 gatgacccaa cactggcctt cctgaatcat taacacttca tcctgccagc cctggtcacc
    22381 ctgtttgaga tccacccgca atggaatgtt acgggcatca cgattaggga tagtcgatgc
    22441 actggcaacg tgggcgctgt ctggcaaggt ggttcgactg gagaacggat cgctgcttaa
    22501 caattcacga tgactattat cgcggctggc atcgctaagt tgggttgcca gtttgtcact
    22561 caaatagggg cgcagggcgg taatgtcatt gctgcgatgt tgaatgcggt agtcgtagaa
    22621 ttgttgagcc acactgtcag gccctccctg gacacaggaa ccggtgcggg ggccgttatc
    22681 tttataagct ggcgtgacgg tggtacaggc actcagcagc agtgcacatg gaataagtag
    22741 agatagtgaa cggtagcgca taatcatttc cttattagct tgctgtctgc gtcacactac
    22801 gaccaaacgc tatcagatta tttaatacat tgtttttaat acaattctga aacaatctga
    22861 acacctgatt gggtttgagt ccattgctgg ctacccactc aaggttgctc gtagctgact
    22921 tacttaaatt acccaaacaa tagacagtgt aaaccatttg tgaggttgag cgtgttatat
    22981 ggctgaaatg atcatttaag gtaaatatga tagttctgaa atggtggtat aaatcctcct
    23041 gtagaggtta agagcatctg caagaagcca gtttcacatt tataacgcgt gataaatgaa
    23101 tgcggttatt gtctaatcat tacaaaattt ttcacatgcg ataagagatc ctgcgattaa
    23161 gatgattgcc aaaattacgt tgtatttttg aaaatgagcg aaatttgata gtcccagctt
    23221 tgactctcga taatattatt tatgctacaa ttgtgggtaa agaatttaag ctaatgaatt
    23281 tgagtgaaga ataaattaaa ttgcttcagt attttgttaa ttaatatgaa tacaaaaggc
    23341 gttgtgcttt tataattata aagcgcgagg agttcggtgg attatcgttt ttcttgggtg
    23401 atttttaata tgataaacaa tatatctgat caagcatcat catttccagg cacacaacta
    23461 aatcaaagtg ataatttcct ggattcactg agggaatttt tcgcgatatt aaattccagt
    23521 cgtaagggtg agttgtctac atgggatacc atctacttgc atctgatatt agcaataaat
    23581 gctgacagtg atctcataaa aaatgatgtg ttgctcgcgg aaaatattcc atcagcgaat
    23641 taccaattta atacattttt tagcaatgct ttcgagattg atgttaaaaa atatctgggt
    23701 aaatcagaag ataacgaagt tgaaataaaa gcgggtaatg aaagaatctc tattggaata
    23761 cgtaatgtat cgaatggaaa acttgaaaga caacagtttc ttttcccact ggactatgag
    23821 aataaattac aagaacaatt agataagtat tttacgattg aatcgcaccc attattatac
    23881 aggtatacca ttggtagtaa gattgcgaat gtgatttttg aaaagttata cagtcgtatt
    23941 gactttaaca aagaacaata tatatccttt ataaaggacg catttattca tttttatgat
    24001 tatagcaggc gatatgctat tagtgagaat atcgataaag acgctgtaac aaacaatatc
    24061 gcattgatga gcacttttta tgacagtgat aatacgtcag gtgaggtttt aaataatgat
    24121 tttacagaag aagagtcatt tgaaactgcg ttagatgtag agcatgcaat agtgcttgga
    24181 tttgctgatg ataattttga aacaaaacct gttcattatc aggatttatt aacacgattt
    24241 agtgcctttc aagataccgt gtttaatctg tttcctgaaa tgcactcttc gcattaccac
    24301 gatatttgta gcgtaagtgt ggatatgaca aagggaacgc aatgcatgat tcacttgatg
    24361 gtgaatgaag aagttttcat gtctctgcct gttccagttg ccacgatggt acgtgaagat
    24421 gcaagtaatt tagtcaatct taaaacacta ctcaatgatg gttgtttcat taaatatagt
    24481 cattttaatg acgtggcatt aataaagcaa aacataagta atctctattt atcacacact
    24541 gttgtgaatg aaagcatatt gaaaaaatgt tgttttgaaa acggcagttt aggtgatgtt
    24601 aaaataacaa atagcaatgt aataaacagt gcattcaaaa atatttcgtt tagatctgtt
    24661 aaaattaata acgtaaatac tcattcttta aaatttatta actgcaattt tttcaatgtt
    24721 gatatgataa gagttaatct cagcaaatgt ctttttcatg aatgtagtat gcatggtgtc
    24781 aagataaaac cctggttgcc tgtaaaatgg acaaaagaat taataagtga ttatttgtat
    24841 ggctgtttac tatctctgta ctctatctgt gcaagggata tatataatat gaacgccggg
    24901 aataacgtga aggttgctgc ggatgctttc cttgaaatta ttttctctct taaaaataaa
    24961 tattgtatta aactattaag cgctcaggat cgggcgttta tatacgagtt tgcaagaatg
    25021 atcttcgctt atattaatga caaaagcatt gagatcttat tactttcttg ttttgcggcg
    25081 gctgaccaaa aagcaatcca gcggtataga cctcagtcac aagatgggga agactttagg
    25141 agtcatttac aatataagtt gcctctttcc gctcattaat gttgttggtg caacataata
    25201 agacaggtaa gcggtaccct ataatgaaac caatatcggg ttatgcaacg gataatcgta
    25261 aagacttaag tgggtgtttt tgacacaatg agatgatttc ctaaactgat caaaaccttc
    25321 ttcccaacat ggcatatcag tccgataatg ctttagagat cgaaggctta acaaacaagg
    25381 agatcgatca tgcaattttc tacaacccca actctggaag gccagaccat cgttgaatat
    25441 tgcggtgtgg tgaccggcga agcgatttta ggtgccaata ttttccgtga tttctttgcc
    25501 ggtatccgcg atatcgttgg cggacgttcc ggtgcgtatg aaaaagaact gcgtaaagca
    25561 cgggagatcg cctttgagga attaggctcc caggcgcggg cgctgggggc cgatgccgta
    25621 gttggtattg atatcgacta cgaaacggtc gggcaaaacg gcagtatgct gatggttagc
    25681 gtcagcggta cggcggtgaa aacgcgtcga tgagaagagt cttctggctg gtcgctgccg
    25741 ctctgttact ggcagggggt gcaggcgaaa aaggcattgt cgagaaagag ggatatcagc
    25801 ttgatacccg acaccaggcg caggcggcgt atccgcgcat taaagtgctg gtgatccact
    25861 acaccgcaga tgattttgat agctcgctgg cgacactgac tgataagcag gtcagctcgc
    25921 attatctgat ccctgcggtg ccaccgcgat acaacggtaa accgcgcatc tggcaactgg
    25981 tgccggaaca agaactggcc tggcatgcgg ggattagcgc ctggcgcggg gcaacgcgcc
    26041 ttaacgacac ctctattggc attgagctgg aaaaccgtgg ctggcaaaaa tcatccggag
    26101 tgaaatattt tgccccgttt gaacaggcac agattcaggc gcttattccg ctggcgaaag
    26161 atattattgc ccgttatcac atcaagccgg aaaacgtagt ggcacatgcg gatatcgcac
    26221 cgcagcgcaa agacgatccg gggccattat ttccctagca gcaactggcg cagcagggga
    26281 ttggtgcctg gccggatgcg cagcgggtga acttttacct tgccgggcgc gcgccgcaca
    26341 catcggtaga taccgcgtca ttgctggagc ttttggcgcg ctacggttat gacgtaaaac
    26401 ctgatatgac accgcgcgag cagcggcgcg tgattatggc attccagatg catttccgcc
    26461 cgacgttata taacggcgaa gcggatgcag aaactcaggc gattgccgaa gcattgctgg
    26521 agaaatacgg gcaggattag cgcggcagtt ttccgtggtc gcgtagccag gcggcagttt
    26581 tctcgatgcc ttcatccagg gtgatgaccg gctgataacc taactcttcc tgcgcacgcg
    26641 taatatccag cgtaaagtca aaattcaact tggagacgcc gtagtgggtc agcggcggct
    26701 cttttgctga cttgcggcct aaacgctcca tgctgcgggc gatcatatcc agcatcgggt
    26761 aagggacgga acgaatacga cagtcaatat tcaactcgtc gatcagcttc tgcacgatgc
    26821 tgcgcagtgt gcgatgctcg ccgttggtga tgttgtacac acgcccggaa ggtagcttat
    26881 cgcaggcttc ctggcttgcc agccacattg cgtgcacggc attttcatag taggtcatat
    26941 cgaccagcgc actgccgcca tgcggtaaca gaatactgcc gtagtggtgc atcatatgcg
    27001 ccagacgggg aataaagact ttatcgtgcg gtccgaacag actttgtggg cgcagaatag
    27061 taaagcgcgt ttgtggattc gcctgcgaaa gcatattgat cacttcttcg ctggctgctt
    27121 tgctgcgggc aaactcgttg gcgaagcggt gagggcgaaa atcttcttta atatcgcgat
    27181 ggtggtgata atcgaagtac agggaggggg aagagatatg aataaagtta cgcacacccc
    27241 aggcgacagc ccattcaccc aggcggcgag tggcgcgaac gttagccaga tcgaaagcct
    27301 gttgtgtccc ccagggtgag gtaaagctgg agcagtgcca cagcgtatca atgcccgcga
    27361 gcatcacttt agcttgtgat gaaaccagct cggtcagatc cgtcggaaca aactctgcgc
    27421 ccattttttc cagcaattta cccattgctt cgttgcgacc gctcgcacgc acgctgatgc
    27481 ctttctggca taaaaactct accgcgtttc gacctaagcc gctggtggcg ccggtaacca
    27541 gtaccttcat atcaatccac tgttgttgag aaaatgacgt gcgcattctt ccgtgatttc
    27601 ccccatgatg caatgggaaa catgaaagaa taacgcaggt tttgtcgatt aatctgtgct
    27661 ttgttctgcc agtctggcga tttgttttgc cattccgcga aaaataaaca gatgcgcggg
    27721 gatcatcaat aaccagtaaa acagccccgg cataccgtgc ggatgccaga aagcgcggac
    27781 atcgatagta cgatagtcac ctttatcttc cagggtaaaa cacagtcgtc ccagccccgg
    27841 cgctttcatg ccaaataaca acgtaagttg ctttttcggt tcaacgacaa tcactttcca
    27901 gctatccacc gcatcgccgg tctgtaaata ttcgcgctcc gggcggcctt tcgccagatt
    27961 atgaccgatc gcgcggtcca tcaacgcccg tgtctgccac aaaatattgc caaagaaata
    28021 acgctcttta ccgccgattt ggttcaccac cagccataaa gccgcaaggc tggcggacgt
    28081 tttaacggta aaccccgcct gtttggcgaa ataaccgtac tccggtcgcc agcgggcaaa
    28141 ggcctgagcg tcgtagcccc agtcgctgga gttgaccagt ttttcttcct ctttcaacgt
    28201 gctacgtacc gcgtcatcga aagcgatcag ccgttgtggg atgagtgcac gtagcgcggt
    28261 atcatctgcc agcagatcgt gtttcagccc ctgaatcaac gccctggcgg tggtgggcgg
    28321 tacggaagta atcacattga gaaaccacac cgaaatccag cgggtgggga gggggatggg
    28381 gatcaaccag cggcgcttac cgctcaccgc cataaaatgt tcaaactgtt gctgataact
    28441 gagcacctct ggtccggcgg cttcgaagat gcggtgttcg ttggctggat ggtctaacag
    28501 cgccaccaga tagtgcagca agttttccag cgcgatgggc gtggtgcgtg aacgtaccca
    28561 gcgtggcggc gttaacactg gcaggttgta gaccatatcg cgcatgactt cgaacgccgc
    28621 tgaacctgcg ccaacgataa ttccggcccg aagttcggtc actggtacat tcgcttcacg
    28681 aagaatgtcc gccgtagcct gacgagcacg cagatgatcc gactgctcat gtggcggggc
    28741 ctgcaacgaa ctgagaaaga ttaattgctt aactggtact tcacgtagcg catcgcggac
    28801 gttgagagcc acttggcgct cctgagcgat aaaatcgccg ccttcgccca tgctgtgcac
    28861 cagaaaatag acggtatcga tatcctgcaa cagggccgga aggttatccg gccagctgag
    28921 atcgacttta tggcaactga cgtttgccag ttgcagcttt gcaagcctgt cgacatgacg
    28981 tgccgccgcc aggatctgat gcccttgctg gctgagtgtg cgcaccagat gctgaccaat
    29041 gtagccactg gcaccgagaa ctaaaatgcg ttgcggcacg tctctctcct taacgcgcca
    29101 ggaatgcgcg ccagtgggcg gcgacttccg ccagttgttc gcgcgagacg tcaagatgcg
    29161 tcaccaggcg gacaatcggc gaggcgttaa tcagcacgtt tctcgctttc atgtattcgc
    29221 ctaacgcggc agcattttct tccccgacgc gaacaaacag catattagtg tcctgacgca
    29281 tcacatccgc gcctgcttca cgcagttgct ccgccatcca ggcggcgttg tcgtgatctt
    29341 cctgcaaccg tgcgacgtta tttttcaggg catacatccc ggcggcagcc agaatcctgg
    29401 actgacgcat cccgccaccg gccattttcc gccagcggat ggcacgttta atgtaatcac
    29461 gattaccgac gagtaatgaa ccgactggcg tcccaagacc tttcgacagg caaatggtga
    29521 acgaatcaca atattgcgtg atctctttca gttcgcagcc gtaagccacc acggcattaa
    29581 agatgcgcgc accgtcaaca tgcagcgcca gattgcgctc gcgggtaaat tcccatgctt
    29641 ctttcaggta ttcacgcggc agcactttgc cgttgtgggt gttttccaga ctgagtaatt
    29701 tggtgcgggc gaaatggata tcgtcgggtt tgattttcat cgccacttta tccagcggta
    29761 gcgtgccgtc ggcagccgcg tcgatgggtt gcggctgaat actgcccagc accgctgcgc
    29821 caccggcttc aaacagatag ttatgcgcgg cctgaccgac aatatactct tcgccacgtt
    29881 cgcagtgact gagcagagcg accaagttgg cctgagtgcc ggtaggcaga aaaatggcgg
    29941 cttctttacc ggaaagctct gctgcatagt cctgcagagc attaacggta gggtcgtctc
    30001 cgtaaacgtc gtccccaacc ggggcggcca tcatcgcttc gagcatggcg cggctcggtc
    30061 gggtaacggt atcactgcgt aaatcaatca tggcatgtcc ttattatgac gggaaatgcc
    30121 acccttttta ccttagccag ttcgttttcg ccagttcgat cacttcatcg ccgcgtccgc
    30181 tgataattgc gcgcaacata tacaggctaa aacctttggc ctgttcgagt ttgatctgcg
    30241 gtggaatggc taattcttct ttggcgacca ccacatccac caacaccgga ccgtcgatag
    30301 agaaggcgcg ttgcagggct tcatcaactt cagaggcttt ttctacacgg atacccgtaa
    30361 tgccgcacgc ttcggcaatg cgggcaaatt tggtgtcgtg cagctcagta ccgtctgtca
    30421 ggtaacctcc ggccttcatc tccatcgcca caaagcccag cacgctgtta ttaaagacga
    30481 taattttcac cggcagtttc atctgcacta ctgagaggaa atcgcccatc aacatgctaa
    30541 aaccgccatc gccgcacatg gcgaccacct gacgttccgg ctcggttgcc tgcgcaccca
    30601 acgcctgcgg catggcgtta gccatcgaac cgtggttaaa cgaacctaac aggcgacgct
    30661 tgccgttcat tttcagataa cgtgccgccc ataccgttgg cgtgccgacg tcacaggtaa
    30721 agatggcgtc atcggcggca aaatgactaa tttgctgcgc cagatattgc gggtgaatgg
    30781 ctttctcgct cggtttagct aaatcgtcca gccctttgcg ggcatcgcgg taatcttcca
    30841 gcgctttatc cagaaacttg cgatcggttt tttcttccac cagtggcagc aatgcgcgca
    30901 gggttgattt gatatcgccg accagcgcca tatctacctt gctatgcgcg ccgatgctgg
    30961 ctggattgat atcaatctga ataattttgg catcggtcgg gtagaacgca cggtagggga
    31021 actgagtacc gagtagcact aacgtgtcgg cgttcatcat ggtatggaaa cctgacgaga
    31081 atccgattaa cccggtcatt ccaacatcat acggattatc gtattcgaca tgctctttac
    31141 cgcgtagggc atgaacaata ggtgctttaa ttttcccggc aaactcaact aactctttat
    31201 gcgcccccgc gcagccgctg ccacacatca gggcgatatt gctggaataa cgcagcagtt
    31261 gcgccagttt gcgtaactct tcttcttccg gcgtcacgac tggctgtggc gcatgatacc
    31321 agtgcgtggt tgccccttct ggcgcaggtt ttaacgccac gtcgcccggt aacacaacaa
    31381 cggaaacgcc acggttaagc accgctttgc gcatagcaat tgccagcact tgtgggatct
    31441 gctccgggct ggaaaccagc tcgcaatagt gactacattc gcggaatagc tcttgtgggt
    31501 gggtttcctg gaaatagccg ctgccaattt cgctggaggg aatatgagcg gcaatcgcca
    31561 gtaccggaac gttattgcgg tggcaatcga ataggccgtt gatcaagtgc aggttgcctg
    31621 ggccgcacga tccggcacag accgccagtt ctcctgaaag ttgtgcttca gcgccagcgg
    31681 caaacgccgc cacttcttca tggcgggtgg acatccactc gatggtgccc atgcgattaa
    31741 gactgtcgct aagaccgttc agagagtcgc ccgtgactcc ccagatgcgt ttcacgcctg
    31801 ccgattcgag tgttttggcg atataagctg caaccgtttg tttcatggtt ctccatctcc
    31861 tgaatgtgat aacggtaaca agtttagttc atctgacgga gggggaaggg atgggagaga
    31921 aaggaggcac taacggttaa atagcccgat gaaaggaata tcatcgggca taaggcgatt
    31981 atgcgagaac caaatccccc tgcggatggc aggagcaggc cagtacgtaa ccttcagcga
    32041 tttcggcgtc ggtcagcgtc attgtactgc tcaccgtata ttcaccggaa accacttttg
    32101 tcttacagca gccgcaaaca cccgcacggc aggcagcgac aaccggaacg ttattgcttt
    32161 ccagcgcctc cagtagcgtg gtgccaaccg gggcgtaaaa ttctcgtgcc ggttgcagtt
    32221 tggtgaattt cagaccgctg gtcgccgctt ctgctactgg ggtgaagaat ttctctttaa
    32281 agaaacgcgt cacgccgagc gctttcactt cctgctctac ccaatccata tacggagccg
    32341 ggccgcaggt catcacggta cgtgaagcta agtcaggtac acctgccagc agttcgcgag
    32401 tgagacgacc agcgataaag ccttcggtaa cgttattttc tgccaccagc gttaccggat
    32461 agttacgcca ctcatcggcg aaaataacat cctgcggcgt acgcacgttg tagatcaccc
    32521 gcacatcggc ctgtggacgg ttcttcgcaa gccagcgacg catcgacata atcggcgtga
    32581 cgccgcagcc tgccgccagc aacaggaatt tatcttctgc tttatcgtcg caggtaaatt
    32641 cccccatcgc gtccgaaagc cagagataat caccgcgttt tacatcgcgc gtcagccact
    32701 gggagccgac accgtcatca atccgccgca cggtcagggt gatatattca ctcacgcctg
    32761 gcgtggagga aatggtgtaa gcacgcagcg tttccgctga gtttcgcacg ctgaccagtg
    32821 catattgccc ggcgcgatat gggtagtgat cgtggcaaat cagggaaatc gtccacacat
    32881 ccggcgtttc ttgcgtaatg tgatgaacct gcatccgcca cgggcattga ttcgttggca
    32941 tcgtcatcga caaactcctt acgcgctcaa cagttgcttc atgtcttctt caacagtggt
    33001 gatagaacgc aggccgaatt tctcgttcag caccgccagc aggtctggtg tcaggaaacc
    33061 aggtgcagtc gggccggtga cgatattttt cacgcccaga gaaagcagcg tcagcagaat
    33121 gacaatcgct ttctgttcaa accaggagag caccagcgac agcggcagat cgttcacacc
    33181 acagcccagt ttctctgcca gagtgacagc cagaataatt gctgagtaag catcgttaca
    33241 ttgacctgca tctaccagac gcggcagacc ttcgatatcg ccaaactcaa gtttgttaaa
    33301 gcgatattta ccacaggcga gggtcaggat caggcagtca tccggcacgc tggtggcgaa
    33361 atcggtgaag tagtggcgct cgccgcgtgc gccgtcacag ccaccaagca ggaagatatg
    33421 acgcagtttt tcacggctaa ccagatcaat cagcgtatcg gctgcaccaa gcagcgtctg
    33481 gcgaccaaag cccacggtga tcaggtgagg aatttcgctg tacgggaagc ccgccatttg
    33541 ttgcgcctgg gcgatgaccg cagagaaatc ttcgccatcc agatgacgca cgccaggcca
    33601 gccaacgatg ctgcgggtcc agatacgatc gtcataagcg cctacggttg ggtcgatgat
    33661 gcagttcgag gtcatcacga tggggccagg gaaacgagcg aactccactt gctgattctg
    33721 ccagccgctg ccgtagttac cgaccagatg cttgaattta cgcagctccg ggtagccatg
    33781 cgcaggcagc atttcgccgt gggtgtagac attaacgccc gtgccttcgg tctgttccag
    33841 caggttgtag agatctttga gatcgtgacc ggaaatcaga atgcatttac ccgccgtcgc
    33901 tttgacgttg acctgggttg gcgtcgggtg accgtattta ccggtttcgc ctgcatccag
    33961 aatgctcatc actttgaagt tcatctggcc gatttccatt gaacactcaa gaagcgcgtt
    34021 catatcggca ggccaggtcc ccagccacgc catgatttta tggtactggg cataaatatc
    34081 gttgtcgtat tgaccgagaa catgcgcgtg ttccatatag gccgccgcac ctttcaggcc
    34141 atacaggcac agcagacgca ggccgagaat gttttcgcca atcgccgctt tatctttgtt
    34201 aggggtaaat tctgctgcct gacgttgcag ctcgccgaga tcatcgctca ccagttgcag
    34261 gtcagccatc gggttatcga cgcgcgcgtt ggcatctaca gccaggcatt gtgctttcag
    34321 cgcctcgcgc aaggcaatcg cttcacgagc gtagccgaca atacgcggag aatcgaagtt
    34381 aacgttggtc agggttgaga aaaaggcacg tggcgcgaag ctgtcaacat cgtggttgat
    34441 gatgccgtat tcacgcgctt ttaccgccca ggcagaaagc ccttgcagcg ccgcgatgag
    34501 taaatcctga aggtcagaag tttccgccgt tttaccacac atcccctgcg cgtatgagca
    34561 gccgtttcct gccggagtac ggatagtttg ttcacattgc acacaaaaca tgatcacacc
    34621 ttttaaagtt atatttaata tacatgttta aggttaagac gcttaacgcg gggataaaag
    34681 ggatttttca tgcaacttta agggagattg atttagcgca attttggcgg cagggatcta
    34741 ccgccagaga ggtattacgc agagaaaaag gcgatgagga tcggcactaa caggctaaga
    34801 ataaaaccgt gaacaattgc cgccgggacc atatccagcc cgccagtacg ttgaagaacg
    34861 ggcagggtga aatccattga tgtggcaccg cataagccca gtgcagtaga gcggctgcgg
    34921 cgaatcagcc cagggatcaa cataatagca atcagttcac gggccagatc attaaaaaac
    34981 gccgcgctcc cgattaccgg accaaaagat tcggtcaata aaataccgga aagagaatac
    35041 cagccgaaac cggaggccat tgccagcgcg gtattgatgg ggagatcaag aataaaggcg
    35101 ttaattaaac caccaattaa tgaactgaca accaccacca cggcgacaat cattccccgg
    35161 cgattaagga caatctgctt taaggtcatg ccattattgc gcaactgaat accaacgagg
    35221 aaaagtagca aaattaacgt gtattcactg gcttcggtcg cgtgttgtaa gaaagccagt
    35281 ccactcagac caatggcaaa accaatcact actacgccgc acagttttag cgactccagc
    35341 gccatcgcaa tacgcgacgg gagtttttct tgctgatggt ggttgcgcca cggcaggcct
    35401 cgctccagcc acatcagggc ggcaatatta cacagtaaaa taacggtaat actgacggca
    35461 gaataatgca gaatcgccaa caggttactg gcgaggttat cgagaaacgc cagactgata
    35521 cccataaaaa agagaataag gtaaaccatc cagcttaata gctgattaat aacttttaac
    35581 gcagcttgtt ggcgaagcgg aatgaggtaa cccacaatca ggggaaccag aatgattaac
    35641 agcccagaaa acatgaaaat ccagtccttg caaagaagaa gtcgaaatgc gcgatgacac
    35701 actactgaaa gcggaaggac gagtaaagtt gcaattaaaa ggaaatgtta tgcatcagag
    35761 ggggattttt aatcctatct gagtgtttag cattgttgcg ctcctttcat gtgcaatgcc
    35821 atataaagcc actggcggag ggagcgcgca gggggcagcc agtcgccgcc gccccc
//
LOCUS       DADLSA010000025        35831 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.025, whole genome shotgun sequence.
ACCESSION   DADLSA010000025 DADLSA010000000
VERSION     DADLSA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.025"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..510)
                     /locus_tag="JN691_001260"
     CDS             complement(<1..510)
                     /locus_tag="JN691_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY7242750.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMMA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFM"
     gene            667..1707
                     /gene="gpr"
                     /locus_tag="JN691_001261"
     CDS             667..1707
                     /gene="gpr"
                     /locus_tag="JN691_001261"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAY7242751.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(1747..2241)
                     /locus_tag="JN691_001262"
     CDS             complement(1747..2241)
                     /locus_tag="JN691_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAY7242752.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            2432..3316
                     /gene="yghA"
                     /locus_tag="JN691_001263"
     CDS             2432..3316
                     /gene="yghA"
                     /locus_tag="JN691_001263"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY7242753.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(3588..4013)
                     /gene="exbD"
                     /locus_tag="JN691_001264"
     CDS             complement(3588..4013)
                     /gene="exbD"
                     /locus_tag="JN691_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAY7242754.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(4020..4754)
                     /locus_tag="JN691_001265"
     CDS             complement(4020..4754)
                     /locus_tag="JN691_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HAY7242755.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            5006..6193
                     /gene="metC"
                     /locus_tag="JN691_001266"
     CDS             5006..6193
                     /gene="metC"
                     /locus_tag="JN691_001266"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAY7242756.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            6333..6992
                     /locus_tag="JN691_001267"
     CDS             6333..6992
                     /locus_tag="JN691_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7242757.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(7032..7931)
                     /locus_tag="JN691_001268"
     CDS             complement(7032..7931)
                     /locus_tag="JN691_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7242758.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGYKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            8125..9288
                     /gene="yqhD"
                     /locus_tag="JN691_001269"
     CDS             8125..9288
                     /gene="yqhD"
                     /locus_tag="JN691_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAY7242759.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHFSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            9393..10220
                     /gene="dkgA"
                     /locus_tag="JN691_001270"
     CDS             9393..10220
                     /gene="dkgA"
                     /locus_tag="JN691_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAY7242760.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            10420..11346
                     /locus_tag="JN691_001271"
     CDS             10420..11346
                     /locus_tag="JN691_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAY7242761.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            11397..11654
                     /gene="yqhH"
                     /locus_tag="JN691_001272"
     CDS             11397..11654
                     /gene="yqhH"
                     /locus_tag="JN691_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAY7242762.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(11697..13916)
                     /locus_tag="JN691_001273"
     CDS             complement(11697..13916)
                     /locus_tag="JN691_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAY7242763.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKVSSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(14027..15439)
                     /gene="ftsP"
                     /locus_tag="JN691_001274"
     CDS             complement(14027..15439)
                     /gene="ftsP"
                     /locus_tag="JN691_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAY7242764.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(15514..16251)
                     /gene="plsC"
                     /locus_tag="JN691_001275"
                     /gene_synonym="parF"
     CDS             complement(15514..16251)
                     /gene="plsC"
                     /locus_tag="JN691_001275"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAY7242765.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(16485..18743)
                     /gene="parC"
                     /locus_tag="JN691_001276"
     CDS             complement(16485..18743)
                     /gene="parC"
                     /locus_tag="JN691_001276"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAY7242766.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAAHG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(19289..19771)
                     /locus_tag="JN691_001277"
     CDS             complement(19289..19771)
                     /locus_tag="JN691_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7242767.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(19824..20216)
                     /gene="ygiW"
                     /locus_tag="JN691_001278"
     CDS             complement(19824..20216)
                     /gene="ygiW"
                     /locus_tag="JN691_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAY7242768.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            20368..21027
                     /gene="qseB"
                     /locus_tag="JN691_001279"
     CDS             20368..21027
                     /gene="qseB"
                     /locus_tag="JN691_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAY7242769.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            21024..22373
                     /gene="qseC"
                     /locus_tag="JN691_001280"
     CDS             21024..22373
                     /gene="qseC"
                     /locus_tag="JN691_001280"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAY7242770.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(22419..22751)
                     /locus_tag="JN691_001281"
     CDS             complement(22419..22751)
                     /locus_tag="JN691_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HAY7242771.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            23070..23651
                     /gene="mdaB"
                     /locus_tag="JN691_001282"
     CDS             23070..23651
                     /gene="mdaB"
                     /locus_tag="JN691_001282"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAY7242772.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            23682..23996
                     /locus_tag="JN691_001283"
     CDS             23682..23996
                     /locus_tag="JN691_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY7242773.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(24044..25936)
                     /gene="parE"
                     /locus_tag="JN691_001284"
     CDS             complement(24044..25936)
                     /gene="parE"
                     /locus_tag="JN691_001284"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAY7242774.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPCFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAALVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(25965..26546)
                     /gene="yqiA"
                     /locus_tag="JN691_001285"
     CDS             complement(25965..26546)
                     /gene="yqiA"
                     /locus_tag="JN691_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAY7242775.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQVVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(26546..27373)
                     /gene="cpdA"
                     /locus_tag="JN691_001286"
     CDS             complement(26546..27373)
                     /gene="cpdA"
                     /locus_tag="JN691_001286"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAY7242776.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(27398..27820)
                     /locus_tag="JN691_001287"
     CDS             complement(27398..27820)
                     /locus_tag="JN691_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HAY7242777.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(27821..28450)
                     /gene="nudF"
                     /locus_tag="JN691_001288"
     CDS             complement(27821..28450)
                     /gene="nudF"
                     /locus_tag="JN691_001288"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAY7242778.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            28655..30136
                     /gene="tolC"
                     /locus_tag="JN691_001289"
     CDS             28655..30136
                     /gene="tolC"
                     /locus_tag="JN691_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAY7242779.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQMKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            30284..30955
                     /locus_tag="JN691_001290"
     CDS             30284..30955
                     /locus_tag="JN691_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAY7242780.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            30961..32121
                     /locus_tag="JN691_001291"
     CDS             30961..32121
                     /locus_tag="JN691_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY7242781.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTNLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(32159..32974)
                     /gene="ygiD"
                     /locus_tag="JN691_001292"
     CDS             complement(32159..32974)
                     /gene="ygiD"
                     /locus_tag="JN691_001292"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAY7242782.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLV
                     ELLAPIPVTLDKEAWGFDHGSWGVLIKMYPDADITMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            33090..33863
                     /gene="zupT"
                     /locus_tag="JN691_001293"
     CDS             33090..33863
                     /gene="zupT"
                     /locus_tag="JN691_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAY7242783.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(33921..34091)
                     /locus_tag="JN691_001294"
     CDS             complement(33921..34091)
                     /locus_tag="JN691_001294"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242784.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(34352..35005)
                     /gene="ribB"
                     /locus_tag="JN691_001295"
     CDS             complement(34352..35005)
                     /gene="ribB"
                     /locus_tag="JN691_001295"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAY7242785.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(35109..35257)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            35319..35669
                     /gene="ubiK"
                     /locus_tag="JN691_001296"
     CDS             35319..35669
                     /gene="ubiK"
                     /locus_tag="JN691_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HAY7242786.1"
                     /translation="MTYPYRTTMVLNTYQYRETTMIDPKKIEQIARQVHESMPKGIRE
                     FGEDVEKKIRQTLQAQLTRLDLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTE
                     IKKQPDPETLPPTL"
BASE COUNT         9011 a         9450 c         8903 g         8467 t
ORIGIN      
        1 cataaactca attgcggcaa agaaatcatt catcagtttg gttggatcaa cctgctgttg
       61 cagctcacga cctttatcat catttcccgg ataacctcca acggaactta atccgtcagg
      121 tgccagggcg atataccccg ccttcgccac tcgccgtgcc acatcttcga tatacggatt
      181 cagtccacga ttctcatgca ccaccaccac ggctggcgtt ttgccgctca tttttgcggg
      241 tttcaccaga taaccccgta cctcgccgtg accatttggc gaaggatacg tgatgtactc
      301 agcaacaatc tccgggtcgg taaactctac ctgagtcgcc agcgcataat ttggcttgag
      361 caaatcaaac aacgctaatg ccatcatccc gcccaccgca tacttcgccg caaggttgag
      421 gaactcacgt ttcgaaattt tcccgtgagc gtaatagtcg tagtaatcca acaactcttg
      481 tgggaaatct ttggcggtta aacgcggcat cgttacattc ctcaactggt gtttttttaa
      541 gcaaagcata agcgcgtgtt tttgcccagt ttttcgtcac tctgtgagcc agactacggg
      601 atacgcgctg gcgaatcgct aaactagaaa cattgtttcg aaattgaacg gtggaaagga
      661 gaggtcatgg tctggttagc gaatcccgaa cgttacgggc agatgcagta ccgctattgc
      721 ggaaaaagtg gtttacgcct gcccgcgtta tcgctcggtt tatggcacaa tttcggtcac
      781 gttaacgcgc tggaatcaca gcgtgcaatc ctgcgtaaag cgtttgattt aggcattacg
      841 cactttgatt tagccaacaa ttacgggccg cctccaggaa gcgcagaaga gaactttggt
      901 cgcctgctgc gcgaggattt tgccgcttat cgcgatgaac tgattatctc taccaaggct
      961 ggctacgata tgtggcccgg cccgtacggc tctggcggtt cacgtaaata cctgctcgcc
     1021 agcctcgacc aaagcctgaa gcgtatgggg cttgagtatg tcgatatctt ttactctcat
     1081 cgcgtcgatg aaaatacgcc gatggaagaa accgcctctg cgctggctca tgcggtacaa
     1141 agcggtaagg cgctgtatgt cgggatctcc tcttactcgc cagagcggac gcaaaaaatg
     1201 gtcgagttgc tgcacgagtg gaaaattccg cttttaattc atcaaccttc gtacaattta
     1261 ctgaaccgct gggtggataa aagcggcctg ctggataccc tgcaaaataa cggcgtgggc
     1321 tgtattgcct ttactcctct ggctcaggga ttgctgaccg gaaaatatct caacggcatt
     1381 ccgcaagatt cacggatgca tcgtgaaggg aataaagttc gtggtctgac gccgaaaatg
     1441 cttaccgaag ccaacctcaa cagcctgcgc ttattgaatg aaatggcaca gcagcgtgga
     1501 caatcaatgg cgcaaatggc gttaagctgg ttgctgaaag atgatcgcgt gacgtcggta
     1561 ttgattggtg ccagccgcgc ggagcaacta gaggagaacg tgcaggcgct gaataatctg
     1621 acatttagca ccgaggagct ggcgcagatt gatcagcata tcgccgatgg cgagctgaat
     1681 ctgtggcagg cgtcgtccga taaatgacct gttaataacg ggcgcgcgaa ccgcgcccga
     1741 atcagatcag tgattatgac gagtcagtcg gtcaagatag cccatcacaa atgcagaaag
     1801 cacaaacgtc agatggataa tgacgtacca catcagtttg ttatccggga catttttcgc
     1861 atccataaag acgcgcagta agtgaatgga agaaattgcc acaatcgacg ctgctacttt
     1921 gtttttcagc gacgttgcgt ccattttccc cagccagttc agcttctctt tgttctcgga
     1981 gatatccagc tgtgagacga aattctcata accggaaaac atcaccatca ccagcaaacc
     2041 gccaaccagc gtcatatcca ccagcgacag caacacgagg atcaaatctg attccgccat
     2101 cgagaagata ttcggcagta cgtgaataat ctcctggaag aacttcagcg ccagggcaac
     2161 taacgccagc gaaaggccaa agtacacggg ggcaagcagc cagcgagaag catacattgc
     2221 attttcaaga aaacgttcca tacattcctg tcgtgtagtt aaaacgggca acagtatatc
     2281 gcaattgagt aaacagcacg caacagcaaa tcaacatata cgttaaacct ttgtcgcata
     2341 attctgaggt ttagggatct tcctggttcc cttatcaacg catcgagagc gtctaccctt
     2401 aaaggacgtt gaacctgaag ggagaaaaac gatgtctcat ttaaaagacc cgaccacgca
     2461 gtattacact ggtgaatatc ccaaacagaa acaaccgacg ccaggcatcc aggcgaagat
     2521 gacaccgata ccggattgcg gcgagaaaac ctatgttggt agcggtcgcc tgaaagatcg
     2581 taaagcactg gtgacagggg gcgattccgg aataggtcgc gctgccgcca tcgcttacgc
     2641 gcgtgaaggg gctgacgtgg cgatcagtta tcttcccgtg gaagaagaag acgctcagga
     2701 tgtgaaaaag atcattgaag aatgcggacg caaagccgtt ctgctgccag gcgatttaag
     2761 cgatgagaaa ttcgcccgtt cgctggttca cgaagcgcac aaggcgttag gcgggctgga
     2821 tattatggcg ctggtcgccg ggaaacaggt tgccattccg gatattgcag acctcaccag
     2881 cgaacagttt caaaagacct ttgccattaa cgttttcgcg ctgttctggc taacccagga
     2941 agcgatcccc ctgctaccga aaggtgcaag tatcatcacc acttcgtcaa tccaggcata
     3001 ccagccaagt ccgcatttac tggactatgc ggctacgaag gcggcgattc tgaactacag
     3061 ccgtggcctg gcaaaacagg tcgcggagaa aggtattcgg gtgaatattg tcgcgccagg
     3121 ccctatctgg acggcactgc aaatttccgg cggacaaacg caggataaga tcccgcagtt
     3181 tggtcagaaa acgcctatga agcgtgcggg gcaaccggcg gaactggccc ctgtatatgt
     3241 ttatctggca agtcaggagt cgagctacgt caccgcagaa gtgcacggcg tgtgcggcgg
     3301 cgagcattta ggttaaaaaa atgcccggtt gtgaaaagca accgggcatc attgtgaatt
     3361 cactcttacc tgatgtggcg aaaacgcctt atccgcctac aaacgcatga agattcaaca
     3421 tgttgcaata gattttgtag gcctgataag cgtagcgcat caggcattga gcacctaatg
     3481 ccggatgcgt cgtgaacgcc ttatccggcc tacaaacgca cgaagattca acatgttgca
     3541 atagattttg taggcctgat gagcgtagcg catcaggcaa ttctacttta cttcgctttg
     3601 gcggtttctt cgccgaccag acctatcttc aggtaacccg cctgatgcag cgtatccatt
     3661 accttcatca acgtctcgta atcgacggtt ttgtccgctc ggaagaagat ggtggtgtct
     3721 ttcttgcctt cggttaacgc attcaacgcc gtaatcattg tttcatcggt gaccggatcg
     3781 ttaccgataa acatcgagtt gtctgccttc accgacagat aaaccggttt ttccgggcgc
     3841 ggctgcggcg tgctggtaga agcaggcaag ttcaccttca catctaccgt cgctaacggt
     3901 gccgccacca taaagataat cagcagaacc aacatcacgt cgataaacgg cgtcacgttg
     3961 atatcatgca tttcgccgtt atcgtcgagg ttttcgttaa gatgcattgc catcggacat
     4021 tatcctgcgc gtaatttttg tgcgacacga accggatgcg cagcggcgct ggcttccaga
     4081 tccaggtcac ggctttgcag caacaatacc tgcgctgcaa catcacccag catcgcttta
     4141 aagccgccaa tctggcgtgc aaatacgtta tagataacga ccgcaggaat cgctgccacg
     4201 aggccgattg ccgttgctaa cagagcttct gcgatacccg gcgcaacgac tgccaggtta
     4261 gtggtctgcg tttgcgcgat accaataaag ctgttcatga tgccccagac cgtaccaaac
     4321 agaccaacga acggagaaat cgcgccgatg gttgccagat agccgttacc gcggcccatt
     4381 tgacgaccca ctgcggcgac ccgacgctcc agacggaagc tggtacgttc tttaatacct
     4441 tcgttatcgt cgctgccttc tgacagttcc agctcattct gcgcttcatt gagcaaatgc
     4501 aggcttaagc ttttgctacc aaaatcagcg gcgatatcgt tggcctggtt taaggaacgt
     4561 gcttcagcca gcagttgctg ctcgcgctta aggcgacgct tctgattgaa gaactctacg
     4621 ctcttactga agaagattgc ccaggtgact acggaggcca aaataagccc aatcatcacg
     4681 cacttaacga caatatcggc gtgctgatac ataccccaga cggaaaggtc cgtctgcatt
     4741 aaattattac ccactgtgta tctccaggac gcaagtcaca aaatctgcgc ataataatat
     4801 caaaacgacg tcgaattgat agtcgttctc attactattt gcatactgcc gtacctttgc
     4861 tttcttttcc ttgcgtttac gcagtaaaaa agtcaccagc acgccatttg cgaaaatttt
     4921 ctgctttatg ccaattcttc aggatgcgcc cgcgaatatt catgctagtt tagacatcca
     4981 gacgtataaa aacaggaatc ccgacatggc ggacaaaaag cttgatactc aactggtgaa
     5041 tgcaggacgc agcaaaaaat acactcttgg cgcggtaaat agcgtgattc agcgcgcttc
     5101 ttcgctggta tttgagagta tggaagccaa aaagcacgcg acgcgcaatc gcgccaatgg
     5161 cgagttgttc tatggacggc gcggaacgtt aacccatttt tccttacaac aagcgatgtg
     5221 tgaactggaa ggtggcgcag gctgcgcgct atttccctgc ggggcggcag cggttgctaa
     5281 ttcgattctt gcttttgtcg aacagggcga tcatgtgttg atgaccaaca ccgcctatga
     5341 accgagtcag gatttctgta gcaaaatcct cagcaaactg ggcgtaacga catcgtggtt
     5401 tgatccgctg attggtgccg atatcgttaa gcatctgcag ccaaacacta aaatcgtgtt
     5461 tctggaatcg ccgggttcca tcactatgga agtccacgac gttccggcga ttgttgccgc
     5521 cgtgcgcagt gtggtgccgg atgccatcat tatgatcgac aacacctggg cagccggtgt
     5581 gctgtttaag gcgctggatt ttggcatcga tgtttctatt caggccgcca ccaaatatct
     5641 ggttgggcat tcagatgcga tgattggcac tgccgtgtgc aatgcccgtt gctgggagca
     5701 gctacgggaa aatgcctatc tgatgggcca gatggtcgat gccgataccg cctatataac
     5761 cagccgtggc ctgcgcacat taggtgtgcg tttgcgtcaa catcatgaaa gcagtctgaa
     5821 agtggccgaa tggctggcag aacatccgca agttgcgcga gttaaccacc ctgccctgcc
     5881 tggcagtaaa ggacacgaat tctggaaacg agactttaca ggcagcagcg ggctattttc
     5941 ctttgtgctt aagaaaaaac tcaatgatga agagctggcg aactatctgg ataacttcag
     6001 tttattcagc atggcctact cgtggggcgg gtatgaatcg ttgatcctgg caaatcaacc
     6061 agaacatatc gccgccattc gcccacaagg cgagatcgat tttagcggga ccttgattcg
     6121 cctgcatatt ggtctggaag atgtcgacga tctgattgcc gatctggacg ccggttttgc
     6181 acggattgta taacattgcc acttttggac aattttgcag acattttatt gtgaaaagtc
     6241 ttaaattgtt gcgtccggga tcaaggcgtc ccggacgatt caggagtaca ataggcagat
     6301 aaaggcttaa acgctgttcc acaggaaagt ccatggctgt tattcaagat atcatcgctg
     6361 cgctctggca acacgacttt gccgcgctgg cggatcctca tattgttagc gttgtttact
     6421 ttgtcatgtt tgccacgctg tttttagaaa acggcctgct gcccgcctca tttttgccag
     6481 gcgacagctt gttgatactg gcaggcgcat tgattgccca aggggttatg gattttctgc
     6541 ctacgattgc gattctgacc gccgcagcaa gtctgggctg ctggctaagt tatattcagg
     6601 ggcgctggtt agggaatacc aaaacggtga aaggctggct ggcacagctt cctgctaaat
     6661 atcaccagcg cgccacctgc atgtttgacc gccacggtct gctggcgctg ctggctggac
     6721 gttttcttgc atttgtccgt acgctgctgc caaccatggc gggaatttcc ggtctgccaa
     6781 accgccgctt ccagtttttc aactggttaa gtggattgct gtgggtcagc gtggtaacca
     6841 gttttggcta tgccttaagt atgattccgt tcgttaaacg ccatgaagat caggtaatga
     6901 cgttcctgat gatcctgcca attgccttgt taaccgctgg cttgttaggc acgctgtttg
     6961 tggtgattaa aaaaaaatac tgtaacgcct gacgattttc cccgttcccg gctgctgtac
     7021 cgggaacgta tttaattccc ctgcatcgcc cgcattcttg ccgcatcttc ccccggcgtc
     7081 acaccgaagt aacgtttaaa ctcacggcta aattgcgatg cgctttcata gccgacgcgc
     7141 atcgctgctg cgctggcctt catgccgtca tggatgatca tcatccgcgc cttatgcaga
     7201 cggtaattct tcaaatactg caacggcgag gtgctggtga cagacttaaa attatggtgg
     7261 aacgccgata cgctcatgtt ggcttctgcc gccagttgct cgacgctcag gttttcggtg
     7321 tatttattct caattcgttt cagcacgcgg ctaatcagac tgaagtgagt ctggcgactg
     7381 accagcgcca gtaacgcgcc gccgcaaggt ccggtcagca cgtagtacag aatttcgcgg
     7441 atgatctgtt tgccgagaat acgcgcatcc agtggtcgct ccatcacgtc gagtaaccgc
     7501 tccgccgcgc ataaaatctc ttctgataac gtggcggagt taatcccgct ggctgccatc
     7561 gacggctgga aatgctcatc ttcgccaatg tccatcaaca gttcctgtaa ctgcaaaata
     7621 tcgacattga gacgcaaccc tgccagcggc acctctgacg tggcataggt ttcgcactca
     7681 aacggcaacg gcaccgtcag cagcaggtat tcattggcat cataacgaaa cacgcgttca
     7741 ttgatataac cgattttata cccggaaaag agaattatga tgccaggctc gtacatcacc
     7801 ggtgtacgtg cgaaaggcgt ctcgccatac aacaaacgca catcgggcaa cagttctgac
     7861 aaactatttt ctttattttt cagtttatta actttatccg ccagcaagcg acaaatctct
     7921 tcacgtttca tatcgcgtaa tttcttagga ataatgcggc aatttgattg tgcgcaattt
     7981 tgtagcattt ctccagcact ctggagaaat aggcaagaca ttggcagaaa tgagcattga
     8041 gagccagggc gctggcgatc acaatgaaaa acatcaggca gatcgttctc tgccctcata
     8101 ttggcccagc aaagggagca agtaatgaac aactttaatc tgcacacccc aacccgcatt
     8161 ctgtttggta aaggcgcaat cgctggttta cgcgaacaaa ttcctcacga tgctcgcgta
     8221 ttgattacct acggtggcgg cagcgtgaaa aaaaccggtg ttctcgatca agttctggat
     8281 gccctgaaag gcatggacgt gctggaattt ggcggtattg agccaaaccc ggcttatgaa
     8341 acgctgatga acgccgtgaa actggttcgc gaacagaaag tgactttcct gctggcggtt
     8401 ggcggcggtt ctgtactgga cggcaccaaa tttatcgccg cagcggctaa ctatccggaa
     8461 aatatcgatc cgtggcacat tctgcaaacg ggcggtaaag agattaaaag cgccatcccg
     8521 atgggctgtg tgctgacgct gccagcaacc ggttcagaat ccaacgcagg cgcggtgatc
     8581 tcccgtaaaa ccacaggcga caagcaggcg ttccattctg cacatgttca gccggtattt
     8641 gccgtgctcg atccggttta tacctacacc ctgccgccgc gtcaggtggc taacggcgta
     8701 gtggacgcct ttgtacacac cgtggaacag tatgttacca aaccggttga tgccaaaatt
     8761 caggaccgtt tcgcagaagg cattttgctg acgctaatcg aagatggtcc gaaagccctg
     8821 aaagagccag aaaactacga tgtgcgcgcc aacgtcatgt gggcggcgac tcaggcgctg
     8881 aacggtttga ttggcgctgg cgtaccgcag gactgggcaa cgcatatgct gggccacgaa
     8941 ctgactgcga tgcacggtct ggatcacgcg caaacactgg ctatcgtcct gcctgcactg
     9001 tggaatgaaa aacgcgagac caagcgcgct aagctgctgc aatatgctga acgcgtctgg
     9061 aacatcactg aaggttccga cgatgagcgt attgacgccg cgattgccgc aacccgcaat
     9121 ttctttgagc aattaggcgt gccgacccac ttctccgact acggtctgga cggcagctcc
     9181 atcccggctt tgctgaaaaa actggaagag cacggcatga cccaactggg cgaaaatcat
     9241 gacattacgt tggatgtcag ccgccgtata tacgaagccg cccgctaagc tttttacgcc
     9301 tcaaactttc gttttcgggc atttcgtcca gacttaagtt cacaacacct caccggagcc
     9361 tgctccggtg agttcatata aaggaggaac gtatggctaa tccaaccgtt attaagctac
     9421 aggatggcaa tgtcatgccc cagctgggac tgggcgtctg gcaagcaagt aatgaggaag
     9481 taatcaccgc cattcaaaaa gcgttagaag tgggttatcg ctcgattgat accgccgcgg
     9541 cctacaagaa cgaagaaggt gtcggcaaag ccctgaaaaa tgcctcagtc aacagagaag
     9601 aactgttcat caccactaag ctgtggaacg acgaccacaa gcgcccccgc gaagccctgc
     9661 tcgacagcct gaaaaaactc cagcttgatt atatcgacct ctacttaatg cactggcccg
     9721 ttcccgctat cgaccattat gtcgaagcat ggaaaggcat gatcgaattg caaaaagagg
     9781 gattaatcaa aagcatcggc gtgtgcaact tccagatcca tcacctgcaa cgcctgattg
     9841 atgaaactgg cgtgacgcct gtgataaacc agatcgaact tcatccgctg atgcaacaac
     9901 gccagctaca cgcctggaac gcgacacaca aaatccagac cgaatcctgg agcccattag
     9961 cgcaaggagg gaaaggcgtt ttcgatcaga aagtcattcg cgatctggca gataaatacg
    10021 gcaaaacccc ggcgcagatt gttatccgct ggcatctgga tagcggcctg gtggtgatcc
    10081 cgaaatcggt cacaccttca cgtattgccg aaaactttga tgtctgggat ttccgtctcg
    10141 acaaagacga actcggcaaa attgcaaaac tcgatcaggg caagcgtctc ggtcccgatc
    10201 ctgaccagtt cggcggctaa cgtgcaaatt ctcccggtgg cggtaatgtt ccgctaccgg
    10261 acttttcaga aatcatttat tcccctcgcg tcccgcccgt tgttactctt ccttgttcag
    10321 gaatgccaaa tataaggaca tcatcatgca gagccggaag ctcttaaaag aacaactcat
    10381 ctatatctgg gataaacgca acggagaggt gaaaaacaga tgaaaataat acttctgttt
    10441 ttagcagccc tggcaagttt taccgtacac gcgcagcctc cttcacagac cgtagaacaa
    10501 acagtccggc agatttatca gaactataaa tcagatgcca gtacgcctta tttcggtgaa
    10561 accggagagc gggcgataac ttcagcgcgt attcaacagg cgcttaccct gaacgacaat
    10621 cttacactac cgggcaatat tggctggctg gattatgatc cggtttgtga ttgtcaggat
    10681 tttggcgatc tggtgttaga aagcgtagcg ataacccaaa ctgacgtcga tcatgccgat
    10741 gccgttgtgc gctttcgtat ctttaaagat gataaagaaa agaccacaca gacactgaaa
    10801 atggtggcgg aaaatggtcg ttgggtcatt gatgatattg tcagcaatca tggcagcgtc
    10861 ttacaagcag ttaatagcga gaatgaaaaa acgctggccg ctttagcttc gttgcagaaa
    10921 gaacagccag aatcctttgt tgctgaactc tttgaacata ttgctgatta tagctggccg
    10981 tggacgtggg tggtttccga ctcttaccgc caggcggtta atgccttcta taaaaccacc
    11041 ttcaagacgg ccaataatcc cgatgaagat atgcaaatag aacggcaatt tatttacgac
    11101 aatccgatct gttttggcga agagtcgcta ttttcacgcg ttgatgaaat tcgagtcctg
    11161 gagaaaaccg ccgattccgc ccgcattcat gttcgtttta cgctgaccaa tggcaacaat
    11221 gaagagcaag agctggtttt acagcggcgc gaaggtaagt gggaaatcgc tgattttatc
    11281 cgcccgaaca gcggcagcct acttaagcag attgaggcaa aaaccgccgc cagattaaag
    11341 caatgagctg aattaaataa caattagccg gaacaataaa taaaagggaa cactatatga
    11401 aaacgatttt caccgtggga gctgttgttc tggcaacctg cttgctcagt ggctgcgtca
    11461 atgagcaaaa ggtcaatcaa ctggcgagca atgtacaaac attaaatgcc aaaatcgccc
    11521 ggcttgagca ggatatgaaa gcactacgcc cacaaatcta tgctgccaaa tccgaagcta
    11581 acagagccaa tacgcgtctt gatgcacagg actattttga ttgcctgcgc tgcttgcgta
    11641 tgtacgcaga atgataaaaa aatccccggc agcatgtcag ttgccgggga ttttttttaa
    11701 cgtccaaccg ccgctttagg gcgtttcttc gcaccagcat tcaccggacg agattgcgta
    11761 gacgacactt tttttgccgt agcaggcgtc tgacgctgag tcgccatcgg cgtatgttta
    11821 gtcaacgccg gacgggtatt gcggttctgg cgacgagctt cacgcatctc ttcaatggtt
    11881 ggcgcaggca ctaagcaatc gcgacggctg ccaatcagat gctttttgcc catcgcttcc
    11941 agcgcctggc ggattaacgg ccagtttgcc ggatcgtggt aacgcaacaa cgctttatgc
    12001 aaacgacgct gtttgtcgcc cttcggtacg aagacgtctt cgctcttata accaatcttc
    12061 gccagcgggt ttttcccggt gtaatacatg gtggttgagt tcgccagcgg cgatgggtag
    12121 aagttctgta cctggtcgag acggaaacga tgctttttca gccacagcgc caaattcacc
    12181 atatcttcat cacgcgtacc ggggtgcgca gagatgaaat acgggatcag atactgctct
    12241 ttacctgcct gtttcgagta ggtatcgaac agctctttaa agcggtcata gctgcccatg
    12301 cccggcttca tcatcttcga caacggccct tcttcggtat gttccggagc aatcttcaga
    12361 taaccgccga cgtgatgggt cgccaactct ttgatatagc gcggatcttc cacggcgatg
    12421 tcataacgta cgcctgaggc gatgaggatt tttttaatgc ctttcagatc acgcgcacgg
    12481 cgatagaggt tgatcgtcgg ttcgtggtta gtgtccatgt gcggacaaat atccggataa
    12541 acgcacgaca aacggcgaca agtttgttca gcgcgtggcg atttgcagcg caacatatac
    12601 atgttggcag ttggcccacc gagatcggaa atcacgcccg taaaacctgg aacggtgtcg
    12661 cggatcgctt cgatctcatt aatgatcgaa tcttccgaac ggctctgaat aatgcgccct
    12721 tcgtgctcgg taatagaaca gaaagaacag ccgccaaagc agccgcgcat aatgttgacc
    12781 gaaaaacgga tcatttcgta agccggaata cgggcattgc cataggccgg atgtggcacg
    12841 cgcttgtacg gcagcgcaaa aacgctgtcc atctcttcgg tagaaagcgg gatagcaggc
    12901 gggttgatcc acacatagcg gtcaccgtgt ttttgcatca atgcgcgggc gcagcctggg
    12961 ttggtttcgt ggtgcagaat gcgcgaagca tgggcgtaca gcactttatc gcccttcact
    13021 ttctcgaaag aaggcagcaa tacgtaggtt ttttcccacg gtttctggcg cggtggctgc
    13081 acgattacag ctttcgcttc ctgctttttc ggcgcaacag gtttgttatc cgcacacggc
    13141 aaatcttcgc catacggatg cgggattggg tcgattttcc ctggggtatc aagacgggtg
    13201 gaatccacac cgctccagcc aggcagcgcc tcttttacga taattgcggt attacgcaca
    13261 tcgcggattt cactaatcgg ctcgcccatc gccagacgat gcgctacttc caccagcgga
    13321 cgttcaccgt taccaaacat cagcatgtcg gctttcgaat ctaccagaac ggaacggcgc
    13381 acggtatcgg accagtaatc ataatgcgcg gtacggcgca ggctagcctc aataccgccg
    13441 aggatcaccg gcacatcttt ccacgcctct ttacaacgct gggtataaac cagcgtggcg
    13501 cgatccggtc gcttacccgc gacattatct ggcgtgtagg catcatcatg acgtaaacgg
    13561 cgatcggcgg tatagcggtt gatcatcgaa tccatgttgc cagcagtaac accaaaaaac
    13621 agattcggtt tacccagacg cataaagtcg tctttgctgc tccagtccgg ctgggcgatg
    13681 atcccgacgc gaaagccctg cgcttccagc atacgaccgc aaatcgccat cccgaagctt
    13741 gggtgatcga catacgcgtc gccagtaacc aaaatgatgt cgcagctatc ccagccaagt
    13801 tgatccatct cttcacgaga catcggcaaa aacggtgccg gtccaaaaca ggcggcccag
    13861 tactgcggcc aggagaacag gtcgcgatcc ggttggatca gggagataga gctcattttg
    13921 cttccagaaa tgataaaaaa ataatcaaag gccggggatt ataagccgga acgaaagaga
    13981 aatcgaaagg tattccatac tcgccctcct cgggcgagta tgaagattac ggtaccggat
    14041 tgaccaacag ttgcccaatc gagccacggt ccgccatttc cagcgtctga ctgttgaagt
    14101 agaacgggaa gtgcgcccag gaaggctgac cgaaataaac aagcagctcc acttgtccgt
    14161 caacccaaac ggtatctttc cagcctctgt cttccgggaa cggcatcgcg ccattcacgt
    14221 tacggatctg gaacattacg ccttcaatat ggaacgcttg cggctcgtcc gcgcgtaccg
    14281 tccagcgttc ccacgttcct tgctgcgcgg tgacatcaat acggttgacg tcccacagct
    14341 gtccattaat acccgggtca tcacccagac tgatatcgcg actgcgaatt ggcgaaccag
    14401 ccatgatttc agttggcagc aagcgcatcg gaagactgtc tgtgaccagc ggcagaaggc
    14461 cggttgggcg cagcgttagc accaaggtag aaaccagaat actggatggc tcaaagaagc
    14521 cgcgaatacg atcaacaatg ctcgccgctt cgccacaggt gatcgacact tcatcgccgt
    14581 tgctcatatc caccagaatc tcgcggcgct cgcccggtgc cagcgaaagt tgcttcaccg
    14641 atacaggagc aggcaggaat ccctgatcgc cagaaatcac atgtaacggg cgaccatcgc
    14701 tcatctgtag ttgatagcga cgagagttcg acgcgttcag cagtcgcaag cgcacccagc
    14761 cacgcgagac ttcaacgtac gggctttgta caccgttaac cagcagcgta tcaccaacaa
    14821 agccgccgct tcccggttcg ttgtattctg gcgtaccaaa gttatccagc cgtttatcct
    14881 ggataatgac cggaaaatca tccacaccat aatggttggg gataggcagc gacttgctga
    14941 cttcatcttc caccagccac attccggcaa ggccgttata gacctgctga gccgtgcggt
    15001 tgggagtatt ggcgtgatac cacagagtag ctgcgttctg gcgaataggc agtacgggtg
    15061 cccagtcagc gtttggcgac atcatccgtg ccggaccgcc catcagcggg cctggtacct
    15121 gtagcccggc caccgtcatt gagacatttt ctgtcaggcg gttgctgtaa ataagcttaa
    15181 catcgtcgcc cttccagacg cggatagtcg gccccaggta acgaccattg attccccaga
    15241 ccgacgcgcg tgtccctggc gtaaatgacc agtgcgcacg ttgtacagtc ataaacagcg
    15301 gttgcccacg gcgagattca agtagcggcg gaacgggtag cggttgctgt tgcccggctg
    15361 cgctggcctt caggggaaca gcgcctgcac aaagtgcaat ccccgatgcc tgaatgaact
    15421 gacgccgact gagtgacata tttgctccat gtaaaactgg ctaaagtaat accgtgcagg
    15481 aaagtgttcc ccgcaaaaat acagattgcc gacttaaact tttccggcgg cttcgcgttc
    15541 tgcgacttct ttatcgagct cggcgatttt ttgttccatt atcgaacgac aatgggcagc
    15601 cagctcacga acctgatctt tgccatactg actgacgtca attggcggca gcatttcgac
    15661 aatcaccaga ccgttgtgca gtcgattaag attaatctta ttcgaagttg tagagacgca
    15721 cacgggaata atcgggacgc ccgccgcaat tgccgcgtga aatgctccag tcttgaacgg
    15781 tagcaggccg cgaccacggc tgcgggttcc ttccgggaac atccagatgg aaatacggcg
    15841 ttttttgaaa tgattcacga cttccgcaat ggtgccgtga gctttagtgc gattgtttct
    15901 gtcgatcaat aagttgccgg ttaaccagta caactgcccg aagaagggga tccacagcaa
    15961 gctcttttta cctaccgtca ccgtcggcgg ttgcacaatg ttcgatgctg tcaccatgtc
    16021 atagttgttc tggtggttag cgatatagat agcattgccg tagctttcag catctgcagg
    16081 tttgcggcac tcaactttca ggccgaacag tggcgcaaga cggccaaaca tatgcccaaa
    16141 ggtggccaca tgtttcgggt tacgcgggct gaaaaggcag taaatggagc cgaatacaca
    16201 gactaagatg ctgtaaatca cggtaataat aagacgaaag atatatagca taacaccctc
    16261 tgaaggtctg agagactggc attgtacgtc acctgaaatc aggttagggc attgttaaaa
    16321 gccgctctct ggaaaagcgt attgttaatc gcaacgcatg aataaacaac ggtttcaagg
    16381 gaatttttat cgtttccggg ggttgaatac gctgccggat gacgacttaa cgtttcatcc
    16441 ggcgttcctt gctagcggga ggaaacagcg ccctccccgg catattactc ttcgctatca
    16501 ccgctgctgg cacggcgagg agagtcgatc tcaacacgat cgatacgctg caaaccgcgc
    16561 atcaacgtac cgcggcgtcc acgttcgcca gtgactttct gtagctcttc cggacgcagt
    16621 ttaattttgc gtttcccaac atgaatggtc agcgtgcttt gcggcggcag aacgtacagt
    16681 tgcgccagac catcctcgcc atgcgcggct tctgccgatg gaatgttgat aatcttgttg
    16741 cctttgccct tcgacagctg cggcagatcg cttaccggga acatcaacat acggcctgcc
    16801 tgagtgattg ccagcagcat atcggaagca tcttcaatca ccaccggcgg cataacatgg
    16861 gcattttccg gtaaggtgat caaagcctta cccgcacggt tacgcgccac cagatcgtta
    16921 aaggtgcaga cgaaaccgta acccgcatcg gaagccatca gcagtttctg atcgtcgctt
    16981 tccatcagca tatggtcaac ggtcgcccca ggcggcaacg ttaatttgcc ggtgagtggt
    17041 tcgccctgac cacgcgccga cggcagcgta atcgggtcga tggcatagct acgaccggtg
    17101 gaatcaacaa acactaccgg ttggttactc ttacctttca ccgccgcttt gaagctatca
    17161 cccgctttat aattcaggcc cggcgcgtcg atatcatggc ctttagcgct gcgtacccag
    17221 cccatctgcg acagcacaat ggtgacaggt tcagacggca gcatgtcgtg ctcgctcatc
    17281 gctttcgctt cttcgcgttc ctgcaacggc gaacgacgat cgtcaccgta ggcttgcgcg
    17341 tctgcctgca gttctttctt cagcaggtta ttcattttac gctcggaagc caaaatgccc
    17401 tgcaactggt cgcgctcttt ttccagctca ctctgctcac cgcgaatctt catctcttcc
    17461 agtttggcaa gatgacgcag tttcagttcg aggatcgctt ccgcctgggt ttccgtaagg
    17521 ccaaaccgcg acatcagcgc cggtttcggt tcatcttcgt tacgaatgat ctcaatcact
    17581 tcgtcgatgt tgagaaacgc caccagcaaa ccttccagga tatgcaggcg cttgaggact
    17641 ttctccagac gatagttcag tcggcggcgc acggtatcgc gacggaacac cagccattcg
    17701 gagaggattt ccagcaggtt tttcaccgcc ggacgaccat ccagaccgat catattgagg
    17761 ttaatgcgat agctcttttc cagatcggtg gtagcgaaga ggtggttcat cacctgatcc
    17821 atatccacgc ggttggaacg cggcacaatc accagacggg tcgggttctc gtggtcagat
    17881 tcatcgcgca gatcgtcaac catcggcagc tttttgttgc gcatttgcgc agcaatttgc
    17941 tccagtacgc gcgcacctga aacctgatgc ggtaatgcgc tgataaccac cgcgccatct
    18001 tctttcttcc acaccgcgcg catacgcact gaaccacgtc cgttctcgta gattttacga
    18061 atctcggcgc gcgaagtgat aatttccgct tcagtcggat aatccggccc ctgcacgata
    18121 tccagcagct gatcgagcgt ggttttcggc tggtcgatta atgcgattgc cgcctgagct
    18181 acttcacgca ggttatgcgg tggaatatcg gtcgccatgc cgacggcaat accggtggtg
    18241 ccgttaagca aaatgtttgg cagacgggca ggtagcattt tcggctcctg caaagtgccg
    18301 tcgaagtttg gcacccagtc agccgtcccc tgccccagct cgctcaatag cagctcggaa
    18361 tatttcgaca accgggattc ggtgtaacgc attgccgcga acgatttcgg atcgtccgga
    18421 gcgccccagt tcccctgacc atcaaccagc ggataacggt aagagaacgg ctgcgccatc
    18481 aggaccatcg cttcataaca ggcgctatcg ccgtgcggat ggtatttacc cagtacgtca
    18541 ccgacggtac gggccgattt tttaaatttg gcgctggcat tcaggcccag ttcagacatc
    18601 gcatacacaa tgcggcgctg aacaggtttc agaccatcac caataaacgg caacgcacgg
    18661 tccatgatca cgtacatgga gtagtttaag taggcgtttt ccgtaaattc atgtagcgca
    18721 aggcgctctg ccatatcgct cattaattct gattcctcaa cttattcgcc agccttcaaa
    18781 ggggcaatat tgccggagat actacattat ctgccgcgtc gagtcacagg agatcagcgt
    18841 gaatagcgcc gccggacgtg ctccgacggc gataaaggca tcaatgtgcc ttgatataca
    18901 actctttgct gtagacttga tcggcataga gggcagcaca aggttgcgcc catgccacac
    18961 cactcggcat gggggtccgc tccgggcgat tcgagaagtt gaggttatgc gagtccaggg
    19021 aagcctaaag catcaaagta gtcgactgta agtatacggt tcagcccagt ttagctgtgt
    19081 ctccaccagt gccccgcgcc tcctgccata agtgcggctt gctcatggat acccccgaga
    19141 cggcttacgg cgcaccggat aataaatttc gcgtatacat ggtattcctt accggagaaa
    19201 ttataagccc aacaacaata cgcctgtgac attgttcgct caaacttatc gcgagtgatt
    19261 tggctcacac tccggtaaag attccccatc acgccaacgg cacataaaca tccgtttcca
    19321 gtcgctgctc tgtcacccct tcggcaagat tggtgtagtg aaacagaatc ggtgctttac
    19381 gcattttctc gccgcttgca ggcagccagt ggcgaataat gccccatatc gtgtgtgaaa
    19441 tatcgtccag ctcgccaacg tggcgggcca cggcataacg tccaccggta agttcaccat
    19501 tgctcacacc ataacgatta tcgggaatcg gttcgctaac gctgccgcag atatcaaagc
    19561 gaaacgcttc cggtgcggtg gtggcagggt cgtcccaggc gacgccaaaa gtctggcttt
    19621 ggttaacagg agaaagcccc gtctctttac gccacatgat aaatttcgcc acactgtagt
    19681 tgagcaactc agggctacag cggtgcggca acatcgccac agggattgat gggaaatcga
    19741 taatgtttac atccagtgtc aggtttgtca ttttgcctcc ctgcgaaatt attcccggga
    19801 acgcgtcccg ggagcggtaa caattacgga tttactttgc ggatctgttt gacgtcaatt
    19861 tcaacagaat tccagtcttt atcgacttca ccctgaatct caaccgtatc tttcggcgtc
    19921 accgtcacac cgttccagcg tttgtgatcg atatcaacat tgatagtacc gctggcatct
    19981 ttaaacacgt agagatcgtc agagatgcgt tcaacgatat tgccgcgcag ggttacccag
    20041 gtgtcgtcac gcagggattt tgcgctttct acagtcgtta cgctgccgtt cggcccctgg
    20101 aatcctccgg cctgactttg cgttgccgat gggccagaaa aaccgccctg ctctgctgcc
    20161 atcaccggtg cactgcacag ggccattact gcgattactg ctgcgaattt tttcatgttt
    20221 attactccct ttaatgtctg tttccgagca tttaacaaga tagtccttaa caacttctta
    20281 agggaaaaaa ataaaattta atgctgtaca gagcgcgtta caacacggtt tactggcagc
    20341 aaatacggtt atcgcaggga tgaaaaaatg cgaattttac tgatagaaga tgacatgctg
    20401 attggcgacg gcatcaaaac tggccttagt aaaatgggtt ttagtgtcga ctggtttaca
    20461 caaggtcgtc agggaaaaga ggcgctttat agcgcacctt atgatgcggt gatcctggat
    20521 ttaaccttac caggcatgga tggtcgcgat attttgcgcg aatggcgaga gaaaggtcag
    20581 cgtgagccgg tactgaacct gaccgcgcgt gatgcgttag cggaacgtgt agaagggctg
    20641 cgtctgggag ctgacgatta tctgtgtaaa ccttttgcgt tgatagaagt cgccgccagg
    20701 ctggaggctc tgatgcgccg aaccaacggc caggccagca acgagctgcg ccacggtaac
    20761 gtcatgctcg accccggcaa acgtatcgcc acgctggctg gcgaaccctt aacactgaaa
    20821 ccaaaagaat ttgccctgct ggaattactg atgcgtaacg ctggtcgggt actgccgcgc
    20881 aaactgattg aagagaaact gtatacctgg gacgaagagg tcaccagtaa tgccgttgaa
    20941 gtgcatgtgc atcatctgcg acgcaaactc ggtagtgatt ttattcgtac cgtgcatggt
    21001 attggctaca cattaggtga gaaatgaaat ttacccaacg tcttagtctg cgcgtcaggc
    21061 tgacgctaat ctttttaatt ctggcctcgg tgacctggct gctttccagc tttgtcgcct
    21121 ggaaacaaac aacggataac gtcgatgaat tgttcgacac ccaactgatg ctgtttgcca
    21181 aacggttaag tacgctcgat ctcaacgaaa tcaacgcggc ggatcgcatg gcacagacgc
    21241 caaataaatt aaaacacggt catgttgatg acgatgcgct gacctttgcc atctttaccc
    21301 acgacggcag aatggtcctt aatgatggcg ataacggaga agatattccc tatagctatc
    21361 aacgggaagg ttttgctgac gggcaactgg tcggtgaaga cgatccttgg cgttttgtct
    21421 ggatgacctc acctgatggc aaatatcgca tcgttgttgg ccaggaatgg gaataccgtg
    21481 aagacatggc gctggcgatt gttgccgggc aattgatccc gtggctggtc gcactgccga
    21541 ttatgttaat catcatgatg gtactactgg gtcgtgaact cgcgccgctg aacaaactgg
    21601 cgctggcact acgtatgcgt gaccctgact cggaaaaacc actaaacgcg actggcgtac
    21661 ccagcgaagt gcggccactg gttgagtcgc taaatcaact gttcgcccgc acacatgcga
    21721 tgatggttcg agaacgacgc tttacctccg atgcagctca cgaactacgt agcccgttaa
    21781 cagcgctgaa agtgcaaacc gaagttgcgc agctctctga cgatgatccg caggcgcgga
    21841 aaaaagcact gctccaatta cattccggga tcgatcgcgc tactcgtctg gttgatcaac
    21901 tgctcacgct atcgcggctg gactcactgg ataaccttca ggacgtcgcg gagatcccgc
    21961 ttgaagatct cctacaatcg tcggtgatgg atatttacca cacggcgcag caggcgaaaa
    22021 ttgacgtgcg actgacactc aatgcccacg gcatcaaacg caccggacaa ccgctattgc
    22081 taagtttgtt ggtgcgaaat ttgctggata acgccgtgcg ctacagtcca cagggcagcg
    22141 tgatagacgt cacgctgaat gctgataatt tcatcgtgag ggataacggc cccggtgtga
    22201 caccagaggc actggcgcga attggcgaac gcttctatcg cccacccgga caaaccgcta
    22261 ccggcagtgg acttgggcta tcgattgtcc agcgaatcgc caagctgcat gacatgaatg
    22321 ttgagtttgg gaatgcggaa caaggtggat ttgaggcgaa ggtaagctgg taacttctct
    22381 gtctatgaga gccgttaaaa cgactctcat agattttact aatagcaaaa tataaaccgt
    22441 ccccaaaaaa gccaccaacc acaaaccaga caaataacga gcgagaatac atatagccaa
    22501 aactcgaaca tttcccgcat aaagagtttt cttaagataa gaataataag tggcgtaaga
    22561 agaaaaaatg ctgcaatcat tgcttgcaat atacgatctt cgcctgaaac ggaaggtata
    22621 ctgcaaatat tgcctcccga ctcaagcatc cagtcatact tattgaaagc aaaaaaagaa
    22681 tctattaacc aataagtaag cattatatat agcaatgctt caaatattgt tttaattttc
    22741 tgctttaaca tatatcagta cgctcataaa aatccattta taattatcaa catgacacgt
    22801 tgatattttc atcttttact tattcaaagc catattttca atccccaaaa aaaccgaacc
    22861 aaacgtgtaa attattccgg tgcgagtaag aattatgagg aatggctatc agtattgtca
    22921 ttttcagaaa atatttatcc tgcatcggtg agtcagagct agatcagact tttgctaaat
    22981 tcgcaaaaga ctttgcacat tttgctaatt tcaccgtacc gctctgtgac gtactatagt
    23041 cgacaaacgt ctcaccttga ggttaaaaaa tgagcaacat cctgattatc aacggcgcga
    23101 aaaaattcgc ccactccaat ggtcaactga acgacaccct gaccgaagtc gcggatggca
    23161 cactgcgcga cctcgggcat gatgtccgca tcgttcgcgc cgacagcgac tacaatgtca
    23221 aagcggaagt acaaaacttt ctctgggctg atgtggtgat ctggcagatg ccaggctggt
    23281 ggatgggcgc gccgtggaca gtgaaaaaat atattgatga tgtattcacc gaaggtcacg
    23341 ggactctgta tgccagcgat ggtcgtaccc gcaaagatcc gtcgaaaaaa tacggttccg
    23401 gcggcctggt acagggcaaa aaatatatgc tttctctaac ctggaacgca ccaatggaag
    23461 ccttcaccga aaaagatcag ttcttccacg gcgttggcgt tgacggtgtg tatctgccgt
    23521 tccataaagc aaaccaattc ctcggtatgg aaccgctgcc gacatttatc gctaatgacg
    23581 tgataaaaat gcctgatgtt ccccgctata ctgaagaata tcgcaagcat cttgtggaaa
    23641 tttttggtta actagagctc aggctttaga aggagttaac catgcttacc gtaatcgcag
    23701 aaatccgtac tcgtcctggt caacatcacc gtcaggcggt attggatcag tttgctaaaa
    23761 tcgttccaac cgtactgaaa gaagaaggtt gccacggcta tgcgccaatg gtggattgcg
    23821 ctgctggcgt gagtttccag tctatggcac cggattctat cgtgatgatt gagcagtggg
    23881 aaagcatcgc gcaccttgaa gcgcatctgc aaaccccgca catgaaggcg tacagcgaag
    23941 ccgtaaaagg cgacgtgctg gagatgaata tccgtattct gcagccaggg atttaatcct
    24001 gccttgtttg cccggccatc ctgaccgggc aatgttcttt cctttaaacc tcaatctccg
    24061 ccatgtcgcc tttctcttgc aaccagttgc ggcgatcttc cgagcgtttc ttcgccagca
    24121 gcatatccat catcgcgtca gtacgctgat cgtcttcatc atcgatagtc aactgcacca
    24181 gacggcgagt gttcggatca agcgtggttt cacgcaattg catcgggttc atttccccca
    24241 gacctttaaa acgctggacg ttcggcttgc ctttcttgcg ttttaattgc tcaagtacgc
    24301 cctctttctc ttcttccgtc agcgcgtaat aaacctcttt cccgagatca atacggtaga
    24361 gcggtggcag tgcgacgtaa acgtgaccgt gtttcaccaa cgcgcggaag tgttttacga
    24421 acaaagcgca gagcagcgtg gcaatgtgca gaccatcaga gtccgcatcc gcgaggatac
    24481 agattttgcc ataacgaagc tggctcagat cgtcgctgtc aggatcgata ccgatcgcta
    24541 ccgaaatatc atgcacttcc tgcgaggcca gcacttcgtc agaagaaact tcccaggtgt
    24601 taaggatctt acctttcagc ggcatgatcg cctgatattc acgatcgcgc gcctgcttgg
    24661 cagatccgcc tgcggagtca ccttccacga ggaacagttc ggtacggtta aggtcctgcg
    24721 cggtacaatc agccagtttg ccaggcagcg ccgggccgct ggtcagcttt ttacgcacca
    24781 cttttttagc cgcacgcata cgacgctggg cgctggaaat cgccatctct gccagcaact
    24841 ccgccgcctg aacgttctgg ttcagccaca ggatgaaggc atctttcacc acgcccgaaa
    24901 ctaatgccgc acactgacgc gaagagagac gctctttggt ctgcccggca aactgcggat
    24961 cctgcatttt aaccgacaga acataggcgc agcgatccca gatatcttcc gccgacagct
    25021 tcacgccgcg cggcagaatg ttgcggtatt cacagaactc acgcatcgcg tccaacaggc
    25081 cctgacgcag accattaaca tgggtaccgc cctgcatcgt tgggataagg ttgacgtagc
    25141 tttcggtcag cagttcaccg ccttccggca gccacagtag cgcccagtcc acagcttcag
    25201 tatcgccagc gaaattaccg ataaacggtt tttctggcag cgtcggtaaa ccgtttaccg
    25261 cttccgccag gtaatcattc agaccgtcct gatagcacca gcgctgttcg gtgttgttga
    25321 tctcatcttt aaaggtaatc tcaacgccgg ggcacagtac tgctttggcc ttcagcacat
    25381 gcgtcaggcg tgaaacagaa aaacacgggc tgtcaaagaa ggtttcatcc ggccagaagt
    25441 gcacgctggt accggtattg cgtttaccgc aagtgccgac aacctgtaaa tcctgcacct
    25501 tttcgccatt ttcaaaggcg atgttataaa cctgaccatc gcggcgcacg ttaacttcta
    25561 cgcgcttcga caaggcgtta actaccgaaa tccccacgcc atgcaggccg ccagagaact
    25621 ggtaattttt gttagagaat ttaccgcctg catgcagacg gcaaagaatc agttcaaccg
    25681 ccggtacacc ttcttccggg tgaatatcca ccggcatccc gcgcccatcg tcaatcactt
    25741 ctaacgactg gtcagcatgt aagattacat ccacgcgttt tgcgtgaccc gccagtgctt
    25801 catcgacact gttatcaata acttcttgcc ccaaatggtt agggcgagtg gtatcggtat
    25861 acatccccgg acggcggcga accggctcaa gcccggtgag tacctcaatg gcatcagcgt
    25921 tataagtttg cgtcatggtt taagttagca attcgagttg atcgtcagag atggtgcaga
    25981 ccaagaaaat cgacgatcgg gttgaaataa tcttcgaagc ccgtgaatgc gtggttgccg
    26041 ccttctatga cagtctggcg gcaggaagcg tagtacgcca ccacctggcg gtaatccagc
    26101 acttcatctc ccgtctgttg cagcagccag atcaaatccg gcgcttccag cgggtcaatc
    26161 tgcatgactt tgagatcgta aatatggcgt gactctagca catattgctg cccggtgtag
    26221 gggttctcgt tctgaccgag atagtccgtc agcaattcaa acgggcgtac cgccgggttt
    26281 accaccactg cgggcagcat aaaacattgt gacaaccagg tggcgtaata tccccccagt
    26341 gacgaaccga caatacccag cgaatcgccg ccatgttcca ggacaatgga ttccagcagt
    26401 tctgccgcgt cggaaggata cggcggcaac tgcggaatga tcatctcaac gtcagggtga
    26461 tgttccgcca gccagttttt taacaagctc gcttttgcag agcgcggcga gctgttgaaa
    26521 ccgtgtaaat aaagaagcgt agacatcagt agccttctga agcggtatca ggttggaaac
    26581 gtgtgtccgc caggcgatgc acctcggtgg tcagcgtgcc atcagcatgt aactcgagag
    26641 tacgccagcc gggcgcgata gtatccagcg taaagttgga acagtgcggt ttaaactgca
    26701 cacaggtcga cggcgttgcc agcaggcggc gaccgttcca gtcgagatcc agctcctgat
    26761 gaatatgacc gcacagcaag tatttgacgt gcggaaactt cgccagcacg gtatccagtt
    26821 cgcccgcgtt acgcagactg tgttgatcga gccaactaca acccgcaggt agcggatgat
    26881 gatgcagcag cagcaacgta tggcgttctg gcgcatcggc cagtttacgt tccagccact
    26941 caagctgaaa ctcgctcagc tcaccgtgcg gcacgccaaa cacctggcta tccagcaaca
    27001 ggatttgcca ttgctcacca ataaacaccc gcttcgccgg ggagataccc gcatcctgta
    27061 acgcgctgta catcgcgggc tggaaatcgt ggttgcccgg cagccagacg cagggcgcac
    27121 gaaaacttgc gatgccttca gcgaaatgct gataggccgc agaggattga tcctgcgcta
    27181 aatcacctgt cgcgacaatc aggtcgaatt cgtgctggtg tggacgaatc gcctccagca
    27241 ccgcctggta gctttcccag gtgtttaccc ctaacagggc ttcgtgcttt tgtgcaaaca
    27301 ggtgagtatc ggtaatttgt aaaatccttc ctctggcctc accagccaga ggaagggtta
    27361 acaggctttc caaatggtgt ccttaggttt cacgacgcta gtaaaccgga atcgccatcg
    27421 ctccatgtgc taaacagtat cgcaaccagt ccgctaaaaa ctggttaatt tgatgctttt
    27481 cgtcgcgttg atgcaacttt ttattaggat aatcataccg cgctttgaag cgaaaaatct
    27541 gctggcttga acacacttca gccaccatcg cgtcatgata cagacgcacc gtcattgacg
    27601 gaaggctcca gtaactgatc gcgggcgcag tctgttctat tgtcaccagg gtagtgtatc
    27661 gggtcgattc cacaatcgtc agccgatatt gtgcgtttgc cacctgatag cttacagttt
    27721 cgccgggtgc gtcattgcgc ggtaacaaac ggcgcaattg tgaaaagttc atctcgcaca
    27781 ggcgcatcat ttcaggaaag tcaggtgtgt aacgcttcat ttatgcccac tcatttttta
    27841 acgcttgatg atgcagctgc agccattgca aagcgatgac cgacgctgcg ttgtcgattt
    27901 tcccctcttc tacccactgg tatgcctgtt cccggcttac cacatgaacg cgaatatctt
    27961 cgttttcatc agccagacca tgaataccgc ttgcggtcgt ggcgtccact tcgcccacca
    28021 taattgacga acgctcactg gtgccccccg ggcttgccag gaaacttaac accggtttgg
    28081 tccgtttgac tatcagtccc gcctcttcaa tcgcttcgcg acgggcaaca tcttccacac
    28141 tttcaccctc ttcaatcatc ccggcaacca tctccagtag ccagggggtt tcgctggtgt
    28201 cgtacgcggc aatccgaatc tgctcaatca gcacaacttc atcacgcact gggtcaaagg
    28261 gtagcaagac tgcggcgtga ccgcgctcaa aaatttcccg ccgtacctca tgactcattt
    28321 gcccgttgaa tagacgatga cgaaatctat aaagatctaa tgaaaaaaag ccgcgataaa
    28381 gtgtttctcg tgcaataatt tctacatcgt ttttgccaaa tgtaacgggc aggttgtctg
    28441 gcttaagcat tgttaatgtc ctggcactaa tagtgaatta aatgtgaatt tcagcgacgt
    28501 ttgactgccg tttgagcagt catatgttaa attgaggcac attaacgccc tatggcacgt
    28561 aacgccaacc ttttgcggta gcggcttctg ctagaatccg caataatttt acagtttgat
    28621 cgcgctaaat actgcttcac cacaaggaat gcaaatgaag aaattgctcc ccattcttat
    28681 cggcctgagc ctttctgggt tcagttcgtt gagccaggcc gagaacctga tgcaagttta
    28741 tcagcaagca cgccttagta acccggaatt gcgtaagtct gccgccgatc gtgatgctgc
    28801 ctttgaaaaa attaatgaag cgcgcagtcc attactgcca cagctaggtt taggtgcaga
    28861 ttacacctat agcaacggct accgcgacgc gaacggcatc aactcgaacg cgaccagtgc
    28921 gtccctgcag ttaactcaat ccatttttga tatgtcgaaa tggcgtgcgt taacgctgca
    28981 ggaaaaagca gcagggattc aggacgtcac atatcagacc gatcagcaaa ccttgatcct
    29041 caacaccgcg accgcttatt tcaacgtgtt gaatgctatt gacgttcttt cctatacaca
    29101 ggcacaaaaa gaagcgatct accgtcaatt agatcaaacc acccaacgtt ttaacgtggg
    29161 cctggtagcg atcaccgacg tgcagaacgc ccgcgcgcag tacgataccg tgctggcgaa
    29221 cgaagtgacc gcacgtaata accttgataa cgcggtagag cagctgcgcc agatcaccgg
    29281 taactactat ccggaactgg cggcgctgaa tgtcgaaaac tttaaaaccg acaaaccaca
    29341 gccggttaac gcgctgctga aagaagccga aaaacgcaac ctgtcgctgt tacaggcacg
    29401 cttgagccag gacctggcgc gcgagcaaat tcgccaggcg caggatggtc acttaccgac
    29461 tctggattta acggcttcta ccgggatttc tgacacctct tatagcggtt cgaaaacccg
    29521 tggtgccgct ggtacccagt atgacgatag caatatgggc cagaacaaag ttggcctgag
    29581 cttctcgctg ccgatttatc agggcggaat ggttaactcg cagatgaaac aggcacagta
    29641 caactttgtt ggtgccagtg agcaactgga aagcgcacat cgtagcgtcg tgcaaaccgt
    29701 acgttcctcc ttcaacaaca ttaatgcttc tatcagtagt attaacgcct acaaacaagc
    29761 cgtagtttcc gcgcaaagct cattagacgc gatggaagcg ggctactcgg tcggtacgcg
    29821 taccattgtt gatgtgttgg atgcgaccac cacgctgtac aacgccaagc aagagctggc
    29881 gaatgcgcgt tataactacc tgattaatca gctgaatatt aagtcagccc tgggtacgtt
    29941 gaacgagcag gatttgctgg cactgaacaa tgcgctgagc aaaccggttt ccactaatcc
    30001 ggaaaacgtt gccccgcaaa cgccggaaca gaatgctatt gctgatggtt atgcgcctga
    30061 tagcccggca cccgtcgttc agcaaacatc cgcacgcact accaccagta acggtcataa
    30121 ccctttccgt aactgatgac gacgacgggg cttcggcccc gtctgaacgt aaggcaacgt
    30181 aaagatacgg gttatctgcc gcattcttcc cccttctcgc ttcaatttcg accagccatc
    30241 ctctattctg atgggtattt accactggtc ccggaagaca aaaatgaaac ggacaaaatc
    30301 catacgccac gcatcgttcc gcaaaaactg gagcgcacgc catctgacac cagtcgctct
    30361 cgcggttgcc actgttttta tgctggctgg ctgtgaaaag agtgatgaaa cagtgtctct
    30421 ctatcaaaat gctgacgact gttcagctgc aaacccaggc aaaagcgccg aatgtaccac
    30481 cgcgtacaac aatgcgctga aagaagccga acgtactgcg ccgaaatacg ccacccgtga
    30541 agactgtgtt gctgaatttg gtgaaggtca gtgtcagcaa gcaccagccc aggctggcat
    30601 ggcaccagaa aaccaggcgc aggctcagca atccagcggg agtttctgga tgccgctgat
    30661 ggccggttac atgatggggc gtctgatggg cggcggcgcg ggatttgcac agcagccgct
    30721 gttctcctcg aaaaacccgg ccagtccggc ttacggtaaa tataccgacg cgacgggtaa
    30781 aaactatggc gcagcccagc caggccgcac catgaccgta ccgaagacgg caatggcacc
    30841 aaaaccggcg accaccacta ccgttacccg tggcggtttt ggtgaatctg ttgccaaaca
    30901 aagcactatg cagcgtagcg caaccggcac ctcttctcgt tcaatgggtg gctgataccg
    30961 atggaaagag tcagtattac cgagcgcccg gactggcgtg agaaagccca cgaatacggt
    31021 ttcaattttc acaccatgta cggcgagccg tactggtgtg aagatgctta ctacaagttg
    31081 accctcgccc aggttgaaaa gctggaagaa gtcaccgccg aactgcacca gatgtgcctg
    31141 aaagtggtgg aaaaagtgat cgccagcgat gagctgatga ccaaattccg cataccaaaa
    31201 cacacctgga gttttgtgcg tcaatcatgg ctgactcacc agccatcgct ttattcgcgt
    31261 cttgatctgg cgtgggatgg cactggcgaa cctaaacttc tggaaaataa cgccgatacg
    31321 ccaacgtcac tatacgaggc ggcgttcttt cagtggatct ggctggaaga tcagcttaac
    31381 gccggtaact tgccggaggg cagcgaccag tttaacagtc tgcaagaaaa actgatcgat
    31441 cgcttcgttg agctgcgtga acagtatggc ttccagttgc tgcatctcac ctgctgtcgc
    31501 gacacggtgg aagatcgcgg aaccattcag tatttgcagg actgcgcaac cgaagctgaa
    31561 atcgccaccg agttcctcta catcgacgag ataggtttag gcgaaaaagg ccagttcacg
    31621 aatttacagg atcaggtgat ttctaacctg ttcaagcttt atccgtggga atttatgctg
    31681 cgcgaaatgt tctccaccaa gctggaagat gctggcgtac gctggctgga accggcgtgg
    31741 aagagcatta tctccaacaa ggcacttcta ccgctactgt gggagatgtt cccgaatcac
    31801 ccgaacctgc tgcccgctta ttttgcggaa gatgatcatc cgcaaatgga aaaatatgtg
    31861 gttaaaccga tcttctcccg tgaaggcgca aacgtgtcga tcattgagaa cggcaaaacc
    31921 attgaagcag cggaaggtcc gtatggcgaa gaagggatga ttgttcagca attccacccg
    31981 ttaccgaaat tcggcgacag ctatatgctg attggtagct ggctggtgaa cgatcaacct
    32041 gccggaattg gcattcgtga agaccgtgcg ttgatcaccc aagatatgtc tcggttttat
    32101 ccacatattt ttgttgaata agccacgata ccggatggca ctcgccatcc ggtaattgtt
    32161 agcctatctg caccgacagc atacttaaac tgcccatttc tataccatcg accggaatgg
    32221 taattggctc ctgcccatcc cacacaccta acacatacaa caacggcaga tagtgctctg
    32281 gcgttgggtt cgataacgcg ccaccttcat ggtcgagata attcaccaga ggatgctgct
    32341 ctaccggccc ttgccacgtc agattcgctt tcacatactc attaaacgac gtcgcccacg
    32401 gatacggtga actatcaccg tgccacttca ctgtgcgcag gttatgcacc acgttaccgc
    32461 tggcgaccaa cattattcct tcatcacgca gcgccgcgag tttgcgcccc atttcgaaat
    32521 gccaggcggc aggtttgcta ctgtcgatac tcaactgcac catagtgata tcggcgtcag
    32581 gatacatctt aatcagcacg ccccacgagc cgtggtcaaa gccccaggct tctttatcca
    32641 gcgtcaccgg gatcggcgct aacagctcaa ccagacgctg tgccagcgca ggcgaacccg
    32701 gagcaggata atgcgtatcg tacagcgcct gcgggaagcc accaaagtca tgaatcgtgg
    32761 gcggcgtctc catcgcggtc actcctgttc cacgggtaaa ccagtgagcc gaaaccacca
    32821 caatcgcttg cgggcgtggc aatgtcatcc ccaacttctg ccagctgcgg gtatacaaat
    32881 tatcttccag cacgttcatc ggactaccgt gacctaaaaa caatgctggc atacgtgttg
    32941 aagacatgat gatatcctta actaaaggtg tcattttgat atcctcacaa tacgcttgtt
    33001 cggcggagta agaacccgga taacaatgat gatgatcatc agttattttg acgatctgcc
    33061 tgaaggtgaa gatttataag gagttgtcga tgtcagtacc tctcattctg accatactgg
    33121 cgggggcagc cacgtttatt ggcgcgtttc tcggcgttct cggacaaaaa ccctcgaacc
    33181 gcttactggc gttttcgcta ggttttgcgg cggggatcat gttgctcatc tcattaatgg
    33241 aaatgcttcc tgccgcacta gcagctgaag gaatgtcgcc ggtgttgggt tatggaatgt
    33301 ttatcttcgg tctgcttggc tactttggcc tggaccgcat gttgccgcat gcgcatccgc
    33361 aggatttaat gcaaaaatcg gtgcagccgt tgccaaaatc gatcaagcgc acagccattc
    33421 tgctcactct cggcatcagt ctgcataact tcccggaagg gattgccacc tttgtcacgg
    33481 cgagcagcaa cctggagctg ggatttggca tcgcactggc cgtcgcgttg cacaatatcc
    33541 ctgaaggtct ggcagtggca ggcccggttt atgcggcaac ggggtctaaa cgcaccgcga
    33601 ttctgtgggc ggggatttct ggactagcag aaattcttgg tggtgtgctg gcgtggttaa
    33661 tcctcggtag catgatttcc ccggtggtca tggcggcaat catggcggcg gttgcaggaa
    33721 ttatggtggc gctctcggtt gatgaattaa tgccgctcgc caaagagatt gaccctaata
    33781 ataaccccag ctatggcgta ctatgtggaa tgtcagtgat gggattcagt ttagtgctgc
    33841 tacaaacggc gggaattggt taaaaacaac atcagaaatt gtccagcaca tggacaactt
    33901 ctgccagctt cacgaaagcc ttattctacc ttttcggctg tctcttctct cgtactgttt
    33961 aagtatttgt tccgcgcttc aagcagtgcg tctctgtcct ggcgaaacgc ccgacaataa
    34021 cgtttcaaat gcaggaaata acccaccaca accagagcaa tcaatacaat ccagtaccag
    34081 gcgataaaca tccttttacc cttaatattt aacaatacta aacatttagc gcataaattt
    34141 cacatatcct ttttcggata tattcatatg gtcgcataat aacggaacaa ttatatgatt
    34201 ttgatcataa tgaaaaagaa tgcctgatat taatgaaatt ttctcataga aagagaagcg
    34261 gaattaacat cttgcatagc agggcaaaat aaaaaccgaa gccagctaca acttcggttt
    34321 cttgattaag gcagtaatta agcagcggtt ttcagctggc tttacgctca tgtgcctgac
    34381 ggtatgccac caggtcttca atagtcacga gcgccatatt gtgtttattg gcaaactcaa
    34441 tgcactctgg tgcacgcgcc atcgtgccat cgtcattagt cagctcacac agcacaccag
    34501 ccggtttaaa gccggccagc gtcatcaaat caatagtcgc ttcagtatga ccgccacgcg
    34561 tcagtacacc acctgcctgc gcgcgaagtg ggaaaacgtg gcccgggcga ttcagatcag
    34621 aaggttttgc gccatcggca atcgctgcgc gaacggtcgt aatacgatca gcggcagaaa
    34681 caccggtagt cacaccttca gccgcttcaa tggtcacggt aaaaccggtg ccataggcgc
    34741 tggtgttatt ttctaccatc attggcagat caagttgttt acggcgatct tcagtgatgc
    34801 acaggcaaac aataccgcta ccgtggcgaa tggtcagcgc catctgctca acggtcatgg
    34861 tttctgccgg gaagatcata tcgccttcgt tttcacggtc ttcatcatca agcaccatta
    34921 caccgcgtcc ttcacgcagc gcagccagtg cattttcaac acgttcgaaa ggcgtaccaa
    34981 aagaggaaag tagcgtctga ttcatggtaa aaaaacctca ctaaaattat ggttaccaga
    35041 atcagggcag tcttaggagt ggcggcatat agccaaaata acgtgagcgg gtccatgccc
    35101 gacagaatcg ttactctctc ccatccggac tctaaccgtc ggccccggaa ttacaccgga
    35161 tctgctgtcc tttgagttcg cacccaaagc gctcgcgggc tttcaactga gttgatttac
    35221 cgccggtggg gaatttcgcc ccgccctgag aataagcgga ttcactataa cgctaatgat
    35281 tagcggcagc aacgcatagc ttcacataat tctggtttat gacttaccct tatcgcacta
    35341 caatggtact caacacctat cagtacaggg aaactacaat gattgacccg aaaaaaattg
    35401 agcaaatcgc tcgccaggtt cacgaatcaa tgcctaaagg aatcagggag ttcggggaag
    35461 atgtggagaa aaaaatccgc caaaccctac aagctcagct gacgcgcctc gaccttgtaa
    35521 gccgtgaaga gttcgacgtc caaacgcaag tcctgttacg gactcgtgag aaactggcgc
    35581 tgcttgaaca aagaatcagc gaactggaaa atcgcagtac tgagatcaaa aagcagccag
    35641 atccagagac tctcccccca acgctgtaaa catttgtctt taatgcactg taaattatga
    35701 attaatcctc taatttacag tgttttctat tacattatcg cttaatccca ttcagagtat
    35761 catcacaaaa atgaatgatt gcaattgaaa atataattgt tatgtattct ttaagtttta
    35821 cttaatcgat a
//
LOCUS       DADLSA010000026        35278 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.026, whole genome shotgun sequence.
ACCESSION   DADLSA010000026 DADLSA010000000
VERSION     DADLSA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.026"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(47..4663)
                     /locus_tag="JN691_001297"
                     /pseudo
     CDS             complement(47..4663)
                     /locus_tag="JN691_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(4756..5403)
                     /gene="rnt"
                     /locus_tag="JN691_001298"
     CDS             complement(4756..5403)
                     /gene="rnt"
                     /locus_tag="JN691_001298"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAY7242787.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5506..5913)
                     /gene="gloA"
                     /locus_tag="JN691_001299"
     CDS             complement(5506..5913)
                     /gene="gloA"
                     /locus_tag="JN691_001299"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAY7242788.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(5994..7079)
                     /gene="nemA"
                     /locus_tag="JN691_001300"
     CDS             complement(5994..7079)
                     /gene="nemA"
                     /locus_tag="JN691_001300"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HAY7242789.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMEE
                     YYRQRASAGLIISDSAQTKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQLWHTG
                     RISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGIVNDF
                     RQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGDSVENRARLVLEVVDAG
                     IEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGE
                     PYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLVARLQ
                     LKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7116..7715)
                     /locus_tag="JN691_001301"
     CDS             complement(7116..7715)
                     /locus_tag="JN691_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7242790.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSSEPLENALAHVKNIIATPAV"
     gene            7818..8057
                     /locus_tag="JN691_001302"
     CDS             7818..8057
                     /locus_tag="JN691_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAY7242791.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8106..9002
                     /locus_tag="JN691_001303"
     CDS             8106..9002
                     /locus_tag="JN691_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAY7242792.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9083..9604
                     /gene="sodC"
                     /locus_tag="JN691_001304"
     CDS             9083..9604
                     /gene="sodC"
                     /locus_tag="JN691_001304"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HAY7242793.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9605..11617)
                     /locus_tag="JN691_001305"
                     /pseudo
     CDS             complement(9605..11617)
                     /locus_tag="JN691_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(11617..12474)
                     /locus_tag="JN691_001306"
     CDS             complement(11617..12474)
                     /locus_tag="JN691_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7242794.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNVAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(12477..12713)
                     /locus_tag="JN691_001307"
     CDS             complement(12477..12713)
                     /locus_tag="JN691_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAY7242795.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            12914..13348
                     /gene="slyA"
                     /locus_tag="JN691_001308"
     CDS             12914..13348
                     /gene="slyA"
                     /locus_tag="JN691_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAY7242796.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(13395..13862)
                     /gene="slyB"
                     /locus_tag="JN691_001309"
     CDS             complement(13395..13862)
                     /gene="slyB"
                     /locus_tag="JN691_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAY7242797.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14136..15245
                     /gene="anmK"
                     /locus_tag="JN691_001310"
     CDS             14136..15245
                     /gene="anmK"
                     /locus_tag="JN691_001310"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAY7242798.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            15343..15672
                     /gene="mliC"
                     /locus_tag="JN691_001311"
     CDS             15343..15672
                     /gene="mliC"
                     /locus_tag="JN691_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HAY7242799.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            15731..16387
                     /gene="pdxH"
                     /locus_tag="JN691_001312"
     CDS             15731..16387
                     /gene="pdxH"
                     /locus_tag="JN691_001312"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAY7242800.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            16516..17790
                     /gene="tyrS"
                     /locus_tag="JN691_001313"
     CDS             16516..17790
                     /gene="tyrS"
                     /locus_tag="JN691_001313"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAY7242801.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            17852..18712
                     /gene="pdxY"
                     /locus_tag="JN691_001314"
     CDS             17852..18712
                     /gene="pdxY"
                     /locus_tag="JN691_001314"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HAY7242802.1"
                     /translation="MKNILAIHSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(18756..19361)
                     /gene="gstA"
                     /locus_tag="JN691_001315"
     CDS             complement(18756..19361)
                     /gene="gstA"
                     /locus_tag="JN691_001315"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAY7242803.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19467..20969)
                     /gene="dtpA"
                     /locus_tag="JN691_001316"
     CDS             complement(19467..20969)
                     /gene="dtpA"
                     /locus_tag="JN691_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAY7242804.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(21580..22215)
                     /gene="nth"
                     /locus_tag="JN691_001317"
     CDS             complement(21580..22215)
                     /gene="nth"
                     /locus_tag="JN691_001317"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAY7242805.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(22215..22910)
                     /locus_tag="JN691_001318"
     CDS             complement(22215..22910)
                     /locus_tag="JN691_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAY7242806.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(22914..23534)
                     /gene="rsxG"
                     /locus_tag="JN691_001319"
     CDS             complement(22914..23534)
                     /gene="rsxG"
                     /locus_tag="JN691_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAY7242807.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(23538..24596)
                     /gene="rsxD"
                     /locus_tag="JN691_001320"
     CDS             complement(23538..24596)
                     /gene="rsxD"
                     /locus_tag="JN691_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAY7242808.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(24597..26435)
                     /gene="rsxC"
                     /locus_tag="JN691_001321"
     CDS             complement(24597..26435)
                     /gene="rsxC"
                     /locus_tag="JN691_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY7242809.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNDTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KAHKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(26428..27006)
                     /gene="rsxB"
                     /locus_tag="JN691_001322"
     CDS             complement(26428..27006)
                     /gene="rsxB"
                     /locus_tag="JN691_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAY7242810.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLQIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDESNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(27006..27587)
                     /gene="rsxA"
                     /locus_tag="JN691_001323"
     CDS             complement(27006..27587)
                     /gene="rsxA"
                     /locus_tag="JN691_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAY7242811.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(27664..28104)
                     /locus_tag="JN691_001324"
     CDS             complement(27664..28104)
                     /locus_tag="JN691_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAY7242812.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(28190..28405)
                     /gene="ydgT"
                     /locus_tag="JN691_001325"
     CDS             complement(28190..28405)
                     /gene="ydgT"
                     /locus_tag="JN691_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAY7242813.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(28678..28803)
                     /gene="blr"
                     /locus_tag="JN691_001326"
     CDS             complement(28678..28803)
                     /gene="blr"
                     /locus_tag="JN691_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAY7242814.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            29046..30086
                     /locus_tag="JN691_001327"
     CDS             29046..30086
                     /locus_tag="JN691_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7242815.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(30122..31123)
                     /gene="add"
                     /locus_tag="JN691_001328"
     CDS             complement(30122..31123)
                     /gene="add"
                     /locus_tag="JN691_001328"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAY7242816.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(31227..32399)
                     /gene="malY"
                     /locus_tag="JN691_001329"
     CDS             complement(31227..32399)
                     /gene="malY"
                     /locus_tag="JN691_001329"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAY7242817.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(32409..34001)
                     /locus_tag="JN691_001330"
     CDS             complement(32409..34001)
                     /locus_tag="JN691_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAY7242818.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVASAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            34176..35204
                     /locus_tag="JN691_001331"
     CDS             34176..35204
                     /locus_tag="JN691_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAY7242819.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGVRVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPATLDDIPVTWVSTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
BASE COUNT         8837 a         9083 c         9170 g         8188 t
ORIGIN      
        1 tgaacgtagc agggaaccac gtccttcagg gcgtggagga tgtcaactat ccccaatcca
       61 gcccttgtgg cgaacttgaa aatcccgcct cacgcagcag cgtaaacatc ggcgtttgcc
      121 ggaccggaag atcattcact tctgttagcg taaagcgcag ccttggttcg cgacgcagtg
      181 cggtggtcag cgcctggaaa atctctgggg cgagtaattc ctccttttcc tgccagacca
      241 gcattttttt gccgccttgc gccagataga gtaacaattt gccgccagaa acgaccacca
      301 gcgcacccgc ccgacgcgtt ggtaccagcg ttgccggatg tgcaggccaa ggcagaagat
      361 ttccccacac attagccgga tcgttggcgg agagcgccac tggtgtatag tggcgtgttt
      421 gcgtggcttg tgtcgccaga tcgcgcaatc ggtcaatagt cagacgttca gcgaattgcg
      481 cgccacccag accttctaca aaacgacctc gcataattcg cccggagtct tccatacttc
      541 gacaaagtgt ttgcatcgat ggaaacccgc cagggatatt ttcggctatc accgcctgac
      601 gactgatgat gccgtagcgg tcgagcatat tttccgccag cgctagcatc ctttcggtat
      661 cgtttagtgg ctccacctgc aataacgacc agcgtccagc cagatttggt gtgttgtaag
      721 atacccgcgg cgagacgggt tgtgcataga caggacgccc acgacgagcc cggtgactgc
      781 ggcgagttga ggtgcgtgcg ttggaactgc tgcgggtgag ggcgcgtaac ggtgcccaaa
      841 tatcgctggt aatgacgcct tgccagacca gcgcccataa cgcctcttgc agtgccgagg
      901 aatcaaccga ttcgccgatt ttatcgcgta tccgttggct gatttgttgt gcaaaccagg
      961 ctcctccgtc agccagaaca gcgattatcg cttgctgcag cgccgaacga ttcgcctgat
     1021 ccgcttcggc gggagtgaac gattctgcgg catatttctg tagatgcagt gccaccaggc
     1081 cgtcatcttc acccagcttt ttttgtcccg accagataac cgctccggtt gccagtaatt
     1141 catcgagcat ttctggcgaa tagtcgcgta cgcgggcagg caggatctgg ctttcccaga
     1201 gtgaggcggg taaaccgact ccggcaagct gttcgatcac ccgcatcacg ccatctacgc
     1261 cctcataaac gcctggctat gttgaggcaa agagcgccgg gctaccatcg gtggcgggta
     1321 atacgccctg acgttccaac agcaatcgcg cataggtcgt ggctgcaacg ggacgcgtcg
     1381 cttctctggc ggcttgcagc gagcgcaaac gcagacgacg aaatacttca tcgctcaccc
     1441 agatgtcttg ttgcagattc atcaccagac cctgttcacg cagttgctga agctgctctt
     1501 cggcaatggc aataccgaga ctaaactcat gagccagttg ttcagccgtg accagagcat
     1561 gagcccggag atagcgcaga aagaggtcgc gaagcgggta actgactcta tgtaaataaa
     1621 tctctggcaa tgactctggt agtcgtacgc cgagggcatc acgcagcctg gcggcatcat
     1681 ccatacaggc aagacgctcc tgtccgctaa tcatcgctgg gaagattcgt tttactgcaa
     1741 gaagattttc cagatacgac gcaacctctt cactgctgcc tgtatgccgt tgcgccaggt
     1801 cttcaacggt cattggcccc agttcgcgca gcaggtcgaa caatccttct tcacctttcg
     1861 ctcttctgcc aggagccagt cgttgcaact cttcttccac ctggcgaatg acctgcgggt
     1921 cgagtaattc ccccggatcg acctgtccga gtagattgcg cagtaactcg ctgtccagcg
     1981 acagtacgga tgcccggcgc tctgccagcg gggcgtcgct ctggtacata aattccgcga
     2041 catagccgaa caataaactt gtggcgaaag gcgagggcgt agtggtcgtt acatcggata
     2101 tttgaatttc gccaccgttc agacgacgca tcaaacgttc cagtgcggga agatcataaa
     2161 catcttgcag acattcgcgt agggtttcga gaatgaccgg aaaatccgga tatccctgag
     2221 cgatttccag caactgactg gcgcgcagcc gttgttgcca aagcggggtg cgatggcccg
     2281 gcgtgcggcc cggcattaat aatgcccgcg cggcgcattc gcgaaaacgg gcggcgaaaa
     2341 gtgccgagct gcctaccgcc tcgcggacaa tttgcagcaa cttttctggt tcaaacaaaa
     2401 aaatcgcggc gtcgggcaat ttaccatcag tgtcaggaat gcgcgcaaca atgccgtcat
     2461 cactggcgac cacagacgcg tcagcgcccc atagggcatg tattcgcccg gcaatcgcca
     2521 gcgcccaggg ttcatgcacc cgtcttccat agggagagtg caaaataata cgccagtcgc
     2581 caatttcatc acggcaccgt tcgaggacca gatggcgact ccccggaacg atgcccgtcg
     2641 cgttgcgttg ctcgtcaatc aacccctgaa tattggcgtt cgtattttct tctgccagcc
     2701 acgggggaat agtcccggaa aagaacgcgc catccgccag taaatgaaga aaatcgccga
     2761 tcatctcgcc taattcagcc ggacgtccgt taccttcacc acgccagaag gggagccggg
     2821 cagaacgacc cggagcagga gtcacaatca cctgatcgcg ggtgatttgc tggatccgcc
     2881 atgaggtagc gccgagagtg ataatgtcgt tcacccgcga ctcatatacc atctcctcat
     2941 ccagttcacc cacccgccgc gaaccggcct tttcttcacc ttcgggtaat aacacgctat
     3001 acatgccacg atccggaatg gtgccgccgc tggtaaccgc caacaattga gcgccaggtc
     3061 gggcggtcaa tatcccggtc tccctgttcc agaccagttt ggggcgaaaa gcagaaaaat
     3121 cgccagaggg atagcgcccg gaaagcatat ccagcgtggc gtcaaaaaca cgtcttggca
     3181 gatctttcca cggtgcggca cggcgtacgc gggagtacca ttcgtctacc tgtaatgcat
     3241 ccatcgccgc ggcggcaacg gtttgctgcg caaggacgtc gagaggatta tgcggtggtg
     3301 tcaggttttc cagcctgcct gcgaacatac actctacaat gactgcggaa tcgactaaat
     3361 cacgccgggt acgggggaaa aacagccctt tagatacacc gccaacctga tgtccggcgc
     3421 gaccaatgcg ttgtaaccca ctggcaacag aaagcggcgt tgccacctga atcaccagat
     3481 ccaccgcgcc catatcaatc cccagttcaa gactggaggt tgcgaccacg cagcgtaatt
     3541 cacccgattt cagcgcctgt tcggtgattg ctcgttgttc tttagagacg gagccgtggt
     3601 gtgagcgggc aataaaaacg tcgctacttt gtacacggtt agaggttgcg ccggaggtcg
     3661 actcgaaatg ggccacatca acggcgatag acggggaacg ctgtaagcgt gcggcgtaaa
     3721 gctcatttaa tcgtgccgtc agtttttccg ccagcccgcg tgaattagta aagacaatgg
     3781 tcgagcgatg gcgcaacact tcatcaagga tacccgtttc aatatatggc cagatggagc
     3841 cttcccggcc ggcatggctg tcttcgccgg tgccgctggc gaccgatgag acatcatcca
     3901 tattggcgac cggtacgaca attcgtatct gcggatggcg cattgcgggc gggttgacta
     3961 ccgtaaccgg gcgatcgcca ccaagaaatg ctgccacatc gctggctgag cgcacagtgg
     4021 cagaaaggcc aattcgctgt gctgaggtgt ggagcagcgc atcgagccgc tccagactta
     4081 acgccagatg cgcaccacgt ttactgcccg ctaccgcgtg gacttcatca ataattaccg
     4141 tttcgacgcc gcgtagcgtt tcgcgcgcgc gggaggtcag catcagatag agtgattcgg
     4201 gtgtggtgat cagaatatcc ggcggattac gggtgagttt gctgcgttcc tgtgcaggcg
     4261 tatcgccagt acggatccct acgcgaagat tgacttccgt ttcgccgcgc cgccgccgtt
     4321 catcggcaat acccttcaac gggatctgca agttgcgctg aacgtcggtg cccagggctt
     4381 ttatcggtga aatatagagg atgcgtgagg ttttacgctt atgcgcctcg cgggtatctt
     4441 cgccgccttc gcggaagagc cgatcgaggg cgtagagaaa tgctgccagc gttttcccgg
     4501 agccagtcgg tgcaatcacc agcgcatgtt cgcctcgcgc cgccacatgc caggtttgcg
     4561 actggacagc ggtcggctgt ttaaaggcgc gaagaaacca gtcgcgggtc gccggtgaaa
     4621 acacgtccgg caggagcgat gaagggtctg gattatctgc cattaacgct attccccgtg
     4681 aatatcatgc ttgatgcgtg gaatagcctc actatagatg cctgaatcat tgcatgtcat
     4741 caggcatcga ctcgattaca cctcttcggc ggcaggtagc ggccagcctc ccagacgttt
     4801 ccagcggttg acgatttcac aaaacagcac agcagtgcgt tcggtgtcgt acagcgcgga
     4861 gtgcgcctgg gtgctgtcga agtccatgcc agcggtctgg caagcctttg acaatacggt
     4921 ttgtccgagt gccagcccgg ccagtgcagc agtgtcaaaa gtggcgaaag ggtggaacgg
     4981 gttacgtttc agcgaggcgc gttctgcggc ggccatcata aagctgtgat caaaattggc
     5041 gttgtgcgcc accataatgg cgcggttaca gccgctcgct ttaatacctt tacgtacaac
     5101 tttaaaaatt tcgtgcagcg cctcgtattc gctgaccgcg ccgcgatcgg gatcgttcgg
     5161 gtcaatgccg ttgaaggcga gggcttctgg ttgcaaattt gcgccgacaa atggttcgac
     5221 gtggaaatgt aatgtggtgt ccggcatcag ccagccttgt tcatccattt tcagggtgat
     5281 ggcggcaatc tcaagcagcg catcggtttt ggcgttaaat ccggctgttt caacatcgat
     5341 cacaacagga taaaaaccac gaaaacggtc gcacagaccg gtaagttgag cgttatcgga
     5401 catctgattc tcttaatacg gataaattgc agcgcgcatt atgacaaata ttacttgtcg
     5461 atgcagtaaa gatgcgggcg cgatgagttc acgcccggca ggagattagt tgcccagacc
     5521 gcgaccggcg tctttctctt cgattaactc aattttgtaa ccgtccggat cttccacaaa
     5581 cgcgataacc gtagtaccgc cttttaccgg acccgcttca cgggtcacgt tacccccgtt
     5641 ttgacggatt ttttcgcacg cttcagcggc gttatctacg ctaagcgcga tgtgaccata
     5701 agcagtgccg agttcgtatt tatccacgcc ccagttgtag gtcagttcaa tcaccgcttc
     5761 ttcggtttcc gggccgtagc caacaaacgc cagtgagtat ttgtattccg ggttttcgct
     5821 ggtacgcagc agtttcatgc ccagcacttt ggtataaaaa tcgatggagc gttgcaaatc
     5881 gccaacgcgc agcatggtat gaagaagacg catcttttta tcctcaaaat ggctaacaga
     5941 ttcaaaatgt tgttttagta tagcggcggc tttacgccgc tcgcaatgtt ggattacaac
     6001 gtcgggtaat cggtatagcc ttccgcgccg ccaccgtaga aactttcggc acgctgtggg
     6061 ttaagctcag ctttgagctg caagcgggcg accagatccg ggttcgcaat ccagtcacga
     6121 ccaaatgcca ccgcatcaat taaccctttg ccgatcagcg tttcagcttt ttctactgtg
     6181 tatgcacctg cgccgataat cggaccgtgg aaacgggcgc gtactttttc gcggaacgca
     6241 tcagtatacg gttcaccccc cgcccaatct ggttctgaca tatgcagata agcaatgccg
     6301 cgtttaccca gttgttcaat cagatacagt gcatcggctt cttcattcgg gccgttatct
     6361 gtgttctgga aagtaccgat tggcgaaacg cgaatgccaa tgcgatcggc accccattct
     6421 tcaatcccgg catcgactac ttccagtacc aaacgtgcgc gattttccac gctgtcgccg
     6481 tactgatcgg tacgatggtt tgaagaaggg gaaaggaact gatgcagcaa ataaccgtga
     6541 gcagagtgga gctctaccag atcaaaaccg gcttcacgcg cgttagcaat ggcctgacgg
     6601 aaatcattga cgatacccgg aatctcttcc agttcaagcg cacgcggcat ggatgtttca
     6661 acgcggatcg cctgaccatt ttcatcgcgc agagaagtac gtgttcccgc gctaagtgct
     6721 gaaggcgcta ccggtgcctg accgccaggt tgcaggctgg cgtgagaaat gcgtccggtg
     6781 tgccacagct gcaaggccat atgaccattt tcagcatgaa cgccagcggt gatttttttc
     6841 catgcggcaa tttgctccgg actatggatg ccaggcgcac ctgcatatcc ttttgtctgg
     6901 gcagaatcac taataatcaa accggcactg gcacgttggc gatagtattc ctccatcaac
     6961 ggggtaggaa tgtcacccgg ttcaatactg cgcagacgcg tcagcggtgc cataaaaata
     7021 cggtttgccg ccgtgatcgc gcccactttc agtggggaat acagtttttc agatgacata
     7081 aaggctcccg gtcgtctggt gataaaaaat gcctgctaaa cggcaggcgt cgcaataatg
     7141 ttttttacat gggccagcgc gttttccagt ggctcggaac tgcgcgaaat tttggcctgc
     7201 agattcgcgc caagccatag tgcgtaaagc acttgtgcct gttgtagcgg ttcgccacaa
     7261 aaggttaaac aatggttctc acggccattt tccagagcct gcgagagcag ggcgatcacg
     7321 ccgcgagcac ctttatccat cgcgctgcgc atatcttctg acagatcgca cacttcggca
     7381 gagagtttta ctgtcaggca accactgatg gttccatgtt ggcaaaactg gttcagtgtt
     7441 tgctggtaat aagccagtat gcggtcgcgg tagttacctt cgccggattg cagcaactca
     7501 gtcagtcgct ggtgatatgc ggcgtaatga cgctcaagca tggcaacgcc aaacgcttct
     7561 ttagagcgaa agtagtgata gaaggaccct ttcggcactt cagcggtttt tagtaattcg
     7621 cttagcccca tcccggtgaa tccacgttgc aggcaaagtt gctcgcccgt cgccaagaga
     7681 tgttcgcgag tatcatgttc ggtgtgtttg ttcatgcgtt ggagtgtagt agaccagtcg
     7741 gtctagtgca agcaaagttg ctactttgga cattaacagc ttcaataatg gtaataatct
     7801 tatctgagga gggcgaggtg gcggagcaat tagagttctt tcccgtccag agcccgtgcc
     7861 ggggaatttg ccagtctgac gaacgcgggt tttgtcgcgg ctgttttcgt agtcgggatg
     7921 agcgttttaa ctggaataaa atgagcgatg gcgaaaaaca agaggtgctg cgcctttgtc
     7981 ggcagcgatt aatgcgcaaa ttacgcgcaa ataagccagc gtcatcagac gaaccggagc
     8041 aaccatcact cttttgaacc taatcttgcg tatactcctc tcatatctga taagaggaag
     8101 cgattatggt tcagcgtatt actattgcgc cgcaaggccc ggagttttcc cgttttgtga
     8161 tgggctactg gcgattgatg gactggaata tgtccgcccg ccagctggtc agttttattg
     8221 aagagcatct ggatctcggc gtgaccaccg tggaccatgc tgatatttat ggtggctatc
     8281 agtgcgaagc ggcgtttggc gaggcactga aactggcacc tcacctgcgt gaacggatgg
     8341 aaatcgtcag taaatgcggt atcgcgacga ccgcgcgtga agaaaacgtc attggtcatt
     8401 acatcactga ccgcaatcac atcattaaga gcgccgaaca gtcgctaatt aatctcgcga
     8461 ccgatcatct ggatctgctg ttaatccacc gaccagaccc gttaatggat gccgatgaag
     8521 tggcggacgc gttcaaacat ctgcatcaga gcggcaaagt gcgtcatttt ggcgtatcga
     8581 actttacgcc tgcgcaattt gccctgttgc aatcacgtct gccgtttacc cttgccacta
     8641 atcaggtgga aatatccccg gtgcatcagc cgttactgct ggatggcacg ctcgaccaac
     8701 tacaacaact gcgtgttcgt ccgatggcgt ggtcctgcct tggtggtggt cgtctgttta
     8761 atgatgatta tttccagccg ctgcgtgatg aactggctgt ggtggcggag gagttaaacg
     8821 cgggctcgat tgaacaggtg gtttacgcct gggtattacg tttaccgtcg cagccgctgc
     8881 caattatcgg ctcaggtaaa attgagcgcg tacgggcagc tgtcgaagca gaaacactga
     8941 aaatgacccg tcaacaatgg tttcgtatcc gtaaagctgc actggggtat gacgtaccgt
     9001 aagcactttt aggaatagcc gccgttcaaa aatgtgtcac tggtttacac ttattcagga
     9061 atgcacaatg aacggaggtc ctatgaaacg ttttagtctg gccattctgg cgctggttgt
     9121 tgcgaccggc gcacaagctg ccagtgaaaa agtcgagatg aacctcgtca cgtcgcaagg
     9181 ggttgggcag tcaattggta gcgtcaccat tactgaaacc gataaaggtc tggagttttc
     9241 acccgatctg aaagcattac cccccggtga acatggcttc catattcatg ccaaaggaag
     9301 ctgccagcca gccaccaaag atggcaaagc cagcgccgcg gaatccgcag gcgggcatct
     9361 tgatccacaa aataccggta aacatgaagg gccagaaggt accgggcatt taggcgatct
     9421 gcctgcactg gtcgtcaata atgacggcaa agctaccgat gccgtcatcg cgcctcgtct
     9481 gaaatcactg gatgaaatca aagacaaggc gctgatggtc cacgttggcg gcgataatat
     9541 gtccgatcaa cctaaaccgc tgggcggtgg cggtgaacgc tatgcctgtg gtgtaattaa
     9601 gtaattacga ggccagcgta ccttgcggtg gtgcttgctc aagttgcgaa agcgagcagt
     9661 acagccgcca gacaattgcc gccagctcac gggcggcagg ttgatgatga cgggcaaggc
     9721 tgtcgcaaat ccgctgtaat tcttcaagtg tggccgccag tgatttttgc tgaacgccac
     9781 gctcactcat cactccccgc aacagtgaaa tacagttatc ccgtactcgc gataacggat
     9841 cggaacgcga ttcccagtcg cgcaattgcc agacaacatg agaacagttc agcagcacta
     9901 caccccagcg taataaccaa cggcgagcca gcgcatcctg gctgttactc aactgactga
     9961 tgtgatgata agtgagcgat tcaaattcgc tttcactcag tgttggatgg cggcttagct
    10021 gatcgacaaa atcccggcgc agcgcgcgaa tatggcggcg gcttttacga gcatccgatc
    10081 ctggacgcag aatggcgaac gctagccaag ccagcgcaac gccaacgatt tttgccagat
    10141 tatcgttaag aaaatcagca aaatcataca ccgggggatt agtgacagcg ataaacgaac
    10201 ccataaaaac aatcagttgc ccccacagtg cggcaaattt gggcatctgc aatttaagaa
    10261 gctgcattgt cgccagcagt ggaaagagaa acagtaaaaa ttgccacaga tcgctaatct
    10321 ggaccatcag gccaaatttg accacaaagc tgaatagcga aagtaacacc agcgtgcgca
    10381 tcagcagtga caacgactta aacggtgctg cgacggcgga gtagagtacg cagctaattg
    10441 ctgccagcgt taatgcattg gcaccggcat cccattgcga tgcaatactc catgcgccaa
    10501 tcatcatcaa cgtacaaaat gtacgcagcc cgctccacat agcttcggcg ttgtcggtat
    10561 gacgagccag agcggatgtc cgtgcgagtc tggcatgatc atctacaccg ctttgcagac
    10621 gatgtaattc ctgactactt tgcagataaa ggcggcaaaa ataacgtagt cgctgccaga
    10681 aggcgacgtg ccggtagtcg gcgacgttgg tcgggcgtag cggggcgata atacgtgcga
    10741 cggtgtaagc atctgtttgc gaactggcga gcgccgtcag caattgttcg agaatttctc
    10801 gcgtggcact tggcggtgag ggccagttga gcaacatacg tcgcaggctg gagatgacac
    10861 tggtcatacg taattgctgg tggagtagcg cattaaggcg cgcgttttgc tggcgaaaac
    10921 gatagtggct ccagaaagcc tggatacgca gcaaattcat ggtcagtatc tgcccaatca
    10981 ccccctcatg tgctgcacga atggcatcgt ttgtttcagg ctgccagagt aaactggcat
    11041 gttccagtaa tcgggcgtgc atgtttttca atgcagttaa aagggcagta gcatcggaac
    11101 tgctaggcag gatcatcatc atcatgccgc cgcacaaaat accgactatc acctcgcaaa
    11161 cgcgcgcctg agcgatatcc cacagctggc tggcctcagt aatattaacc atcggaaagg
    11221 cgatgatggc agccgtgtag cccgccagtt gaaatgcata cgcgacgtta ttcgtgaagt
    11281 gcgcacaggc ctaggtacaa aagccaagcc acgccgacat gctcaataga aaaaaccacg
    11341 gctcattgag cgtatgtccg gcaagaagca gtgccgcaat ggctcccagt aaactgccag
    11401 cgatgcgtcc gaggcttttg ctgatgacac cgccaacggt gggaaagcta accactgcag
    11461 ccgaggtcat cgcccaatag ggttcatcca gattcaaata ataggcaacc gtcagcgcca
    11521 gacacatggc aatggtattg cgtaacgcat aacgccattg cgccagcgtg gccctgaacc
    11581 agggcaaatt gcgcaaggac catgacgatg cgttcattat tgacctacgg caatgctaca
    11641 ggtggtgccc gacaccagcg tgacatcgcc tggaacttta tccagcgcaa agcggacggg
    11701 aacgcgttgg gcgagacgaa cccaggggac gttaggtttc acatccggaa taaggcttga
    11761 gtcactttca acgctctgat cataaatcgc ccgaccgata ctggaaacgt gaccctgtaa
    11821 cgttttgtta tcactataga gggtaatttg tgctggtgca ccttcgcgga tatggcgcaa
    11881 cttggtttct tcaaaataac caatgacgta aaacgagtgg ctgtcgacaa gggcaaacag
    11941 cggtttcccg gtgtcagcgt aatcgccgat gcgggtagtg aggttagtca cccatccact
    12001 caccggggcg cgaatttccg tttgcgccag ttgccattgt gtctgtttca gcgtcgcctg
    12061 tgcgacatta acactggcct gcatcgcttt aacattgagg ttagcggtat cgagttcttc
    12121 ggcaaaaata aaattttgtg agagatgacg acgacgattc gcttcgttat tggcttttgc
    12181 gaggtcagat tgcgctttcg ccagctgagc ctgtgcgttc agctcggcga tctgaaaggg
    12241 cgttttatcg atcgtgagca gaagttcccc cgcgttaacc agttggttgt cttttatatt
    12301 cagctcaaca atacgtcccg acacctgtgg ggtgatagaa acctgttctg cgcgtatttt
    12361 cccgtcgcgt gtccacggag attgcatgta ataattccac agccaccatc cggcaagaac
    12421 cgcaactatc gctacaatga ttgttgagaa atacttaatt gttttaattg acatagttac
    12481 cacgcaatca gtattgccag agcaaggcaa acgcaaatcg caaacagcga taaatccatt
    12541 aacaagggat gccagatgtc accggcgtag atccagccac gaagcaggcg gtgtacgaca
    12601 agccagatga cgaatccaaa cgcgaatgct ttgaaaaacg gaggaaaata gacggacgca
    12661 ccgaacacca gatcttgtaa gggcaatcct gttgcattga gcataaactt cacaaagtag
    12721 attcctttac gaccgaatat attgcgttag attaataaat attctttaag tgcgaaaaat
    12781 ttacgcgcaa tttctgaaaa atatgtctga cggtaaccaa atgcagcaat acatttgttt
    12841 tagcaataca attgctgcac actattctaa aagccgcata atatcttagc aagctaatta
    12901 taaggagatg aaattggaat cgccactagg ttctgatctg gcacggttgg tgcgcatatg
    12961 gcgtgctctg atagaccatc gcctgaaacc gctggagtta acacaaaccc attgggttac
    13021 gttacacaat atccatcagt tacctccaga ccagtcgcaa attcaactgg caaaagcgat
    13081 tggcatcgag cagccatcac tggtccgtac tctggaccaa ctggaagaaa aagggttaat
    13141 ttcgcgtcaa acttgtgcca gcgatcgtcg ggctaaacgt attaaactga cggaaaaggc
    13201 agagccactg atcagcgaaa tggaagctgt tattaacaaa acccgcgcgg aaatattaca
    13261 tggcatctcc gcagaggaac tggagcaact gattacgctc atcgcaaaac ttgagcataa
    13321 tatcattgag ttacaggcca aagggtgaaa tgaagggggc gtgtggtcag taacctgacc
    13381 acacgcaagc ttatttagcg cggagaaacg gtcacctgac tgccattgct ggccagtacg
    13441 acacgttggc ccggagagaa acgagtgttg ccttgtttct gtaccaccat gatggtatta
    13501 ccatcgtctt tacgaatttc cagctcgaca ccctgcgttt tgttcattgc actctgtacg
    13561 ccctgaccag ctacgccacc tgcaacagcg cctgctgcag tagccagaga acgcccggtt
    13621 ccgccaccaa cagtattccc caggaaacca ccaagaacag caccgccaat tgcaccgata
    13681 acgttggaat catcaccgcc ctgaatctgt accggacgta cgttaacgat ggtgccatag
    13741 ctgacattct gtacttgttt cgcttcagaa gcggtataaa catcccctga cagggtgtcg
    13801 ttattaacac aaccgacaag agacagacct accattgaaa caaccaatac gcgtttaatc
    13861 attgaaaaat ctcctgttca ccatgaaacg ccacgcaagc atccctcatg gtcaaagtat
    13921 atggcatatg tgaatccatg gttacatgtt ctaccaaata tgagcaaatc ctaatgaaag
    13981 taggatcaac caattataaa caaaacattc atgatgatta ttgatttgaa gataatgttt
    14041 tactaccaac ggtaaaaatc ggtcattccg catggtattc acgaaaagtt tatgtttgag
    14101 tggtgcgcaa acgtcagatt gaaaaaggat gaactatgaa atcgggccgc tttattggcg
    14161 ttatgtcagg caccagcctt gatggtgttg atgttgtgtt ggcgacaatt gatgaacacc
    14221 gggtcgcaca gctggcaagc ttgagctggc cgatcccggt gtctctgaaa caggccgtac
    14281 tggatatttg ccagggccag cagcttacac tttcgcagtt tggacagctt gatactcaac
    14341 tcgggcgact ttttgctgat gcggtcaatg ccttgcttaa ggaacaaaac ctgcaggcga
    14401 gagatatagt tgcgatcggc tgtcacggtc aaaccgtctg gcatgaaccg acgggcgtgg
    14461 caccacacac tttacagatt ggcgataaca atcaaattgt ggcacgcacc ggaattacgg
    14521 ttgtcggtga ttttcgccgt cgcgatattg ccttgggagg acaaggcgca ccgttggtac
    14581 ctgcgttcca tcatgccttg ctggctcacc caaccgagcg acgaatggtg ctcaatattg
    14641 gcggcatcgc caatctgtca ctgctcattc ctgggcagcc ggttggcggc tacgataccg
    14701 gtcctggtaa catgctgatg gatgcctgga tctggcgtca ggcaggtaaa ccttacgata
    14761 aagatgccga gtgggcacgg gcgggtaaag ttattctccc actgctgcaa aatatgctca
    14821 gcgacccgta tttctcgcaa cctgcaccga aaagcaccgg acgcgaatac tttaactacg
    14881 gctggctgga gcgccattta cgccattttc cgggtgttga tccccgtgat gttcaggcca
    14941 cactggcaga actcaccgcc gtgaccattt ctgaacaagt tttgttgagc ggtggctgcg
    15001 aacgattgat ggtatgtggt ggaggtagtc gtaatccgct actcatggcg cgtctggcgg
    15061 cattactgct aggcacagaa gtcaccacca ccgatgccgt tggcattagt ggcgatgaca
    15121 tggaagcatt ggctttcgcc tggcttgcct ggcggacgct ggcgggatta ccaggaaatc
    15181 tgccttccgt cactggcgca agccaggaga cggtactggg ggctattttc cccgccaact
    15241 cgtgatatca gaattaactg aattttttct tatcgtgtct gccgttacac ttggaagatt
    15301 aaggagggta tttgccctcc ggactaggaa agtcttcggg atatgactat gaaaaaactg
    15361 ttaattatca ttttgccggt ccttctctcc ggttgtagcg cctttaatca actggttgag
    15421 cgaatgcaaa ccgatacgct ggaataccag tgtgatgaaa aaccgttgac ggtcaaactg
    15481 aataatccgc gccaggaagt cagttttgtt tacgataatc aactactgca tctcaaacag
    15541 ggcctttcag cctctggcgc gcgttactcc gacgggatct atgttttctg gtcgaaaggt
    15601 gaggaagcga ctgtctataa acgcgaccgc atcgtcttga ataactgtca gttacaaaat
    15661 ccacagcgtt gagatttttc caagggcggc gcacaatagc gtcacccact gacaatccgt
    15721 aaagaaaacc atgtctgata acgacgaatt gcagcaaatc gcgcatctgc gccgtgaata
    15781 caccaaaggc gggttacgcc gccgcgatct tcccgccgat ccattaaccc tttttgaacg
    15841 ttggctctct caggcttgtg aagccaaact ggcggaccct accgcgatgg tggtcgctac
    15901 cgtggatgaa catggtcagc cttatcagcg catcgtttta ctcaaacatt acgacgaaaa
    15961 aggcatggtg ttttacacca acctcggtag ccgtaaagca catcaaattg aaaataatcc
    16021 gcgcgttagc ctgctgttcc cgtggcatac ccttgaacgc caggtgatgg tgatcggtaa
    16081 agcagaacga ctttcgactc tcgaagtgat gaaatatttt catagccgcc cgcgtgatag
    16141 ccagattggt gcatgggttt cgaagcagtc cagtcgcatt tctgcccgcg gtatccttga
    16201 aagtaaattc ctggagctga agcagaagtt tcaacagggc gaagtgccat tgccgagctt
    16261 ttggggcggt tttcgcgtca gccttgaaca gattgagttc tggcagggcg gtgagcatcg
    16321 cctgcatgac cgctttttgt accagcgtga aaatgatgcg tggaagattg atcgtcttgc
    16381 accctgaaaa gatgcaaaaa tcttgcttta atcgctggta ctcctgattc tggcacttta
    16441 ttctatgtct ctttcgcatc tggcgaaaag tcgtgtaccg gcaaaggtgc agtcgttata
    16501 tacatggaga ttttgatggc aagcagtaac ttgattaaac aattgcaaga gcgggggctg
    16561 gtagcccagg tgacggacga ggaagcgtta gcagagcgac tggcgcaagg cccgatcgcg
    16621 ctctattgcg gcttcgatcc taccgctgac agcttgcatt tggggcatct tgttccattg
    16681 ttatgcctga aacgcttcca gcaggcgggc cacaagccgg ttgcgctggt aggcggcgcg
    16741 acgggtctga ttggcgaccc gagcttcaaa gctgccgagc gtaagctgaa caccgaagaa
    16801 actgttcagg agtgggtgga caaaatccgt aagcaggttg ccccgttcct cgatttcgac
    16861 tgcggagaaa actctgctat cgcggcgaac aactatgact ggttcggcaa tatgaatgtg
    16921 ctgaccttcc tgcgcgatat aggcaaacac ttctccgtta accagatgat caacaaagaa
    16981 gcggttaagc agcgtctcaa ccgtgaagat caggggattt cgttcactga gttttcctac
    17041 aacctgttgc agggttatga cttcgcctgt ctgaacaaac agtacgatgt ggtgctgcaa
    17101 attggtggtt ctgaccagtg gggtaacatc acttctggta tcgacctgac ccgtcgtctg
    17161 catcagaatc aggtgtttgg cctgaccgtt ccgctgatca ccaaagcaga cggcaccaaa
    17221 tttggtaaaa ctgaaggcgg cgcagtctgg ttggatccga agaaaaccag cccgtacaaa
    17281 ttctaccagt tctggatcaa cactgcggat gccgacgttt accgcttcct gaagttcttc
    17341 acctttatga gcattgaaga gatcaacgcc ctggaagaag aagataaaaa cagcggtaaa
    17401 gcaccgcgcg cccagtatgt actggcggag caggtgactc gtctggttca cggtgaagaa
    17461 ggtttacagg cggcaaaacg tattaccgaa tgcctgttca gcggttcttt gagtgcgctg
    17521 agtgaagcgg acttcgaaca gttggcgcag gacggcgtac cgatggttga gatggaaaag
    17581 ggcgcagacc tgatgcaggc gctggtcgat tctgaactgc aaccttcccg tggtcaggcg
    17641 cgtaaaacta tcgcctccaa tgccatcacc attaacggtg aaaaacagtc cgatcctgaa
    17701 tacttcttta aagaagaaga tcgtctgttt ggtcgtttta ccttactgcg tcgcggcaaa
    17761 aagaattact gtctgatttg ctggaaataa tgcattaagt ggaaaggggg agtgagaaat
    17821 cactccccct ggtttttata cagggaacat gatgaaaaat attctcgcta tccactctca
    17881 cgttgtttat ggtcatgcgg gtaacagtgc ggcagagttt ccgatgcgcc gcctgggcgc
    17941 gaacgtctgg ccgctgaaca ccgttcaatt ttctaatcac acccaatacg gcaaatggac
    18001 tggctgcgtg atgccgccca gccatttaac cgaaattgtg caaggcattg ccgccattga
    18061 taaattacac acctgtgatg ccgtcttaag tggctatctg ggatcggcgg agcagggtga
    18121 acatatcctc ggtatcgtcc gtcaggtgaa agcagcgaat ccgcaggcga aatatttttg
    18181 cgatccggta atgggtcatc cggaaaaagg ctgtatcgtt gcaccgggtg tcgcagagtt
    18241 tcatgtgcgg cacggtttgc ctgccagcga tatcattgcg ccgaatctgg ttgagctgga
    18301 aatactctgt gagcatgcgg taaataacgt cgaagaagcg gttctggcag cgcgcgaact
    18361 cattgcgcaa gggccacaaa ttgtgttggt taaacacctg gcgcgagctg gctacagccg
    18421 tgaccgtttt gaaatgctgc tggtcaccgc cgatgaagcc tggcatatca gccgtccact
    18481 ggtggatttt ggtatgcgcc agccggtagg tgttggtgat gtgacgagcg gtttactgct
    18541 ggtgaaactg cttcaggggg caacgctgca ggaggccctg gaacatgtga ccgctgcagt
    18601 ctacgaaatc atggtgacca ccaaagcaat gcaggaatat gagctgcaag tggtggctgc
    18661 tcaggatcgt attgccaaac cagaacatta cttcagcgca acaaagctct gaaatatctc
    18721 atttcggccc acaacgacct gtgggccgat ggctattact ttaagccttc cgctgacagc
    18781 gcgtcttgta cttccggacg ttcagccata cgttgcataa atgccgcaat gtgctctaac
    18841 ccttccagat tcagtttcac cgcgtatgcc cagcgcagaa ccgtaaacag ataggcatca
    18901 gcaattgtaa atctttgccc gcagatccag tgctcatcct tcagtgcctc gttcacatat
    18961 tgcagcttct tatccagctg cgcgcgaact gtcggtttgt actcttccgg tgtatctggg
    19021 cgaaacagag gtgtgaaacc tttatgcagc tcggtggcga tgtaattcag ccattcgatg
    19081 gttttatagc gggaaatact gtttaccggt gccagcaact ggcggtcggg gacgctgtcg
    19141 gcaagatact gcataatcgc aacgccttcc gtcagcaaag taccatcgtc cagcagcaat
    19201 gcaggcacct gccccttagg gttaacggca aagtaatcgt caccgttttc gagacgtttt
    19261 ttcattaaat ccacactgac gagcgtaaaa tcctttccgc tctcacgcag ggtgatatgg
    19321 gaagcgagag agcaggcgcc cggtttgtag aacaatttca tcggtaactc cttttgtctg
    19381 tggtttaaag tatggtagtg cgttgctgaa caaaaaaaaa gccgctaacg aaaagttagc
    19441 ggcttgtaaa agagtttccc tgaattttac gctacagcag ctttcgccgc tttgtctgca
    19501 gcgtcatcct gcgtcatgcg gtgcagttta ggcgcggtca gcagcatcag tactgcaata
    19561 acggcagtag cgacaccaat ctgcaagaat acgcgaccat agacttccag tgacatcagc
    19621 gggtcggtaa cgttatccgg cacagccatc atacccgcaa cataaccacc aatcaggttt
    19681 gcaccggcag tggtcaggaa ccagctaccc ataatgaagc ccatcagacg ctgcggaacg
    19741 agttgtgcaa ccattgccag acccagacca gagatcatca gttccccgat gctctgcagg
    19801 ccatagcttg cgaccagcca gctgacagac acgatacctg cgtcagacgc gaatttcgca
    19861 cccagcggca gaatcaggaa cgcaccagaa cacatcacca tgccgattgc aaacttggtt
    19921 ggcatcggca gggtatcacc catcttgtta tagatagcgg ccagaatcgg actaccgatg
    19981 atgatccaga acgggttcag tgcctgatac tgctcaggtt ctacggccag acccagaatg
    20041 gagtgctcaa cgttacgaat cgcaaagaag ttcagtgacg ttggcatctg gctgtacagc
    20101 acgaagaaga tgattgcttc gagcatcagg atgaaggcaa cgatcatttt acgacgcgca
    20161 gcacctttca tcgcgaaagc ttctttaccg aagataacca caataccgaa ggcaacaacg
    20221 cccagcgcca tacgcgcaac ttcctgattg tgcagtagcc aggtggcaac agcgatcagc
    20281 gccacaacac caataatggt cagcaacagg ttacggtagt tgactggttc aaagtctggt
    20341 tttgaaccgt actgtttaac ccagcgttgg cagaaggcga agttaacgat agtgatcagc
    20401 aggcctacaa cgctcaacgc aaacgcaaca ctccagccgt atttcgcggc cagccacggg
    20461 gtggctatca tagagaagaa agagccgatg ttgacggaca tgtagtacat ggtgaatgca
    20521 ccgtccagac gtgggtcgtt tttctcatag catgtagaaa gcagagaaga cgggttagct
    20581 ttaaacaggc cgttaccgac cgcaatagcc gccataccca tataaacaat accggcgtcg
    20641 tgaccagacc aggcaaccag agcataacca atcgccagca caatagcgcc gagcataatt
    20701 acgcgtttag tacccagtac cttgtcacct aaccagccgc cgatagcgac cagaccataa
    20761 accagggcac taaaggaaga gaaaagggtg attgaatccg cttcagacat acccagttgt
    20821 ttaaccaggt aaacagccat aattccttgt aggccgtaat aaccaaaacg ttcccataac
    20881 tcaatcgaga agatgagata gaacgccttc ggttgtttga aagcgttcaa actgacgctt
    20941 tcagttggtt tttggtttgc agtggacact tttacctctt tttttacatc ccatattaac
    21001 gggggtgctt tctcgcgtta tgtctaaaaa acatacgcgc aattgttata gtgggagggg
    21061 aaacggcagg taatgttcac tatcttgcac tttcagacaa gtcctttgta ataatctgtt
    21121 acatataact gaggcgggga attcaaccat tccgggaaat gttatccagc gttaaatttt
    21181 tcaaaaaacg taacggcaga ttttttcact gcattgagag ggggatgatg gcttgacggc
    21241 gatatgttct gctatttagc gtttttccta gtgagatagt ccagtttctg aaaaatagcc
    21301 agtgtaatgt tttgcaggtc aatatttgtt gcgtgtgtaa atttaaatca ttttaacctg
    21361 attcttgcaa tgtttttaca ttgccacagc atcgtgatct tgatcacgaa tgaatagaaa
    21421 atttcgctat taaaaaaaca attaacctgc atttttggtt atccgcaaac ggattacctg
    21481 atgataacgc taagcaatgg catcattgac gttaaaaaga tgttttaaaa gaggataaag
    21541 aaaggttatc aatggggtaa tcggtgttac cccttttctt cagatgtcaa ctttctcttt
    21601 gtattcacaa agatcttcaa taatacaaga gccacagcgg ggcttgcggg caatgcaggt
    21661 ataacgcccg tgcaggatca accaatggtg gcagtcgact ttaaactctg ccggaaccac
    21721 tttcagtagc ttttcttcta cctgttcgac gtttttcccc ggcgcaaatt gagtacgatt
    21781 acaaacgcgg aaaatgtgcg tgtcgacagc aatagtcggc cagccgaatg cagtgtttaa
    21841 tacgacgttg gctgttttac gacctacgcc gggcagggct tcaagcgcag cacgatcttc
    21901 cggaacctcg ccattatgtt gttccagtaa tatacggcag gttttgatga tattttctgc
    21961 tttgctgtta taaaggccaa tcgttttgat ataggttttc accccttcaa cgcccagttc
    22021 aagcatcgct gcaggcgtat tcgccaccgg gtagagtttc gccgtcgcct tattaacact
    22081 gacatcggtc gcctgagcgg aaagcagtac ggcaatcagc aattcaaaag gcgaactgaa
    22141 attaagctcg gtggtgggat gaggattgtt ctcacgcagg cgagtgagga tctccagacg
    22201 ttttgctttg ttcatcagac attccctgtt tcaccgtttg gcaatgcacg ttcggcagct
    22261 gcttcagcac ggcgcttttt cattctttca tcaatcaggt attttcctgc cagcatcagt
    22321 cccaagccaa taaatgcacc tggtggtagc atcgccagca ggaaagggga gtcggtgtgg
    22381 aaaatctcca cgcgtaatac ttttgcccag ctacctaaca gcgcatctgc accgtcaaac
    22441 aacgtgccat tgccgataat ttcgcgtagt gaacccagca cgaacatggc gcaggttgcg
    22501 cccgtaccaa ttgaaaagcc gtccagtgcc gaaagcgccg gacctttttt ggcggcgaag
    22561 gcttcagcgc ggcccacaac gatacagtta gtgacaatca gcggaataaa aatccctaat
    22621 gattgataca ggccaaaggc gtaggcgttg atcagcatct gtacagcgct gaccaccgag
    22681 gcgatgatca tcacgtaaat gggaatgcgg atctcggctg gcgtccagtg acgcagcgtc
    22741 gaaatggtca ggttggtcag cgtcagtacc agcgtagtcg caagtcctaa acccagagcg
    22801 ttagtggcag tggacgtgac cgccaatagc ggacaaaggc cgagcagttg gaccagtgca
    22861 gagttgtttt tccacaaccc ctgaacaata acgtctttaa tttcgctcac gctttattct
    22921 ccacaggcag gaagttgaga aagttgtgcc ggtaacgtct gagcgtacaa tccggcgcgt
    22981 tttaccgcat taaccaccgc gcggggagta atcgtcgcgc cggtgaactg gtcgaaatca
    23041 ccaccatctt tcttcaccgc ccagtgcgca tcatccgcac cactgatttt ttttcccgca
    23101 aaatgggtga tccagtcaga aaggcgcaat tcgattttat cgccaagccc tggcgtttcg
    23161 tggtgctctg tcacgcgcgt gccaagtacc gtgccattaa aatcggctcc caccagcagc
    23221 tgaatcgcac cggaatagcc atctggcgcg gttgcttcca gaacggcggc taccggtttg
    23281 tcatcctgtt tggcgatgta aacccgatgc tcacctttac ctaactctgg cgcagttacc
    23341 agatagcaac tctgtgcgag cgcattgtta tagcgttcgg ctggcagcac ctgatcaaat
    23401 aacgcctttt gttgcagact ggcctgttca gcaattgtcg ttttggtcat ctggttgatg
    23461 gccgcagtta accctgtaga acccgctgca aatagtgcca gtgtaatgcc gtgttttcgg
    23521 atagttttca gcatggttta ccctttgcga tggccgtaga cgcgcggacg cgtgtagtaa
    23581 tcgatcagag gaaccgtgat gttcgccagc aggacggcaa aagccacgcc gtcaggatag
    23641 ccgccgaaac tgcggatcaa ccagactaat aagcccgcca gcgcgccgaa catcagccga
    23701 ccacgattgg tcgtagaagc agtaactggg tcagtcaaaa taaagaatgc gccgagcata
    23761 gtcgcaccag acagcagatg aatttgcggt gctgccagtg tttctggtga gaacaaccag
    23821 cccaacgttg cgcataacgc cagcgttact aagaagctga ggggaatatg ccagcgaatc
    23881 gctttctgcc atagcaacca cacgccacca gccagccagg cgagatttac ccattgccaa
    23941 ccagcgcccg ccagaatacc gctgtagatc ggatattgca taatctgttc aaccgaatga
    24001 cctgcgcgga cagaggtttt gaatgtatcc agcggtgtcg cctgactaat gccatcaata
    24061 cctaagcgta gcgtattcat atcaccacca ctggcggtat gaccgctgaa aataacctgt
    24121 atggcgtcga taaaaccagg gatgttgacc gcaatttcat gtggcggtaa ccagctggtc
    24181 atctgcaccg ggaaggagat cagtaagacc acataaccaa tcattgccgg attaaacggg
    24241 ttttgcccca gaccgccata caactgttta gcgatgatca ccgcaaacac cgtacccagt
    24301 acgaccatcc accatggcgc taggggggga atacttaccg ccagcaataa gcctgtcagc
    24361 aatgctgagt tatctttcaa cgttgcggct accgactgct tgcgtagttt gagtacgaga
    24421 gcttcggcta acagagcact gaccgacgcc aacaggatct gaacgagagt accccaacca
    24481 aaaaaccaca gttgcgctgc gattcctggc acggctgcga gcaacaccaa cagcataatg
    24541 cgcgatgtct ggcgctggtt atgggtataa ggggagctag ctattctgaa taccatttag
    24601 tcctcgttta caaccttttg ctgggcggct tttttggcct gaacgcgggc aatagccgcg
    24661 gcaactgcgg ctttgcgcgg atcgacctgt tcttctggtt ccgcattagc ctgttgctgt
    24721 tccagcttgt gcgctttgga gcgggcgata gctgcttcaa cggcagtttt acgtggttca
    24781 gcaacggttg ctgcgtcgtt agtttgctcc agttctgcct gtttcgctct ggcttgcgct
    24841 ttacgggctt cccgtgctgc aataattgca ctgttatccg ggcgttcgcc cgctttaatc
    24901 acaataggct gtgtagcctg ggcctgtttc tctttcaccc gcgccagagc ggcagcaatc
    24961 gcatctttat ctttggctgc aggttgaacg gctgcgctct tatgtcgttc aaggcgagcc
    25021 gctttttcgc gctccagacg agcctggcgc gcttcgaaac gcgctttggc ttctgcggcg
    25081 cgcttttctt cctgacgaat agccgcaatt tcagcttttt cctgacggaa atattgcacc
    25141 aagggaatat tgctcgggca aacccacgcg caagccccac attcaatgca gtcggcaatg
    25201 ttatgcgtgg tagctttatc gtgttgctga cctttgctga accagtacaa ctgttgcggc
    25261 aaaagatcca cagggcaggc gtcagcacag gcgctacacc ggatgcagct ttgttcttcc
    25321 tgtggttcgc caagttcatt ggcagaggga gccaacagac agttggtaat ctttacgacc
    25381 gggacatcca gccatggcaa ggtaaagccc attagcgggc cacccataat caccatttga
    25441 tcggcagagg ggcagaatcc ggcatcattc aataaatgac gcactggcgt ccccagccgt
    25501 gcccagacgt tgcccgggcg agcgattgct tcgccagtca gggttacaac acgctcggta
    25561 atcggctcgc catcaataac ggcacgtttc actgcataag cagtgccgac gttttgcatt
    25621 aatacgccga tatcggatga acgcccgcca tgtggaacct gcttcccggt cagaatgtag
    25681 gttaattgtt tagcaccgcc agaaggatat ttggttggaa tcacccgcag agaaatatcg
    25741 ttagagtccg ccagcaccgc gcgcagcatg gaaatcgcct gcggtttgtt atcttcaatg
    25801 ccgataagaa tttcgcgtgg ctgcagaata tgcgcaagaa tgcgaatacc ctctacgacc
    25861 tgagccgcgc aatcctgcat caaacggtca tcggcggtaa tgtacggctc gcactcagca
    25921 gcgttgataa tcaacgtttc aatcttatct ccaccaccct gcaatttaac gcctgtcggg
    25981 aatccggcac cgcccagccc ggcaacacca aactgatgta tgcgctcgat taactcttcg
    26041 cgactgcgag agcgataatc gacccagccg tcgcgcggga tccagcagtc ttcaccatcg
    26101 gcatcaataa tcacgcttaa ttcagctaaa gctgaaggat gagccgtaga gtggggcgca
    26161 atagccgtaa cggtacccga ggtgggcgcg tgaacaggca gcattttgcc gcgaccacgg
    26221 gtaagcggct ggccgcgcaa tactttatcg ccgacgctaa cgcacaactc accttcagcg
    26281 ccaatatgct gtttcagtgg aataacaaaa cgctgcgcca gtggtacctg gcgcaggggg
    26341 gtatcgttgg actgggtttt catctccggt ggatggatgc cgccgttgaa atcccagatt
    26401 ttattttttc tgaatgcaga gaataactta agcatggtgt tccacgggaa tgatacgcac
    26461 gggaatggtg ttcagatccc atttccagga gtcaggtgtt tctgcgaccg gttgcaacga
    26521 gatgcagtgc gtcgggcacg gatcaacaca taaattgcag cccgtacaga gatcactcat
    26581 taccgtatgc atggcacggg tagcgccaac gatggcgtct accggacaag cctgaataca
    26641 tttagtgcag ccaatacagt tactttcatc aataaccgcc accatccgcg caggcgttag
    26701 ctcttgcgct tcgccatcca gcggctgcgg ctcgacatta agcaactcgg caatttgtag
    26761 catcacagct tcgccacctg gggcgcaacg gttgattttt tcaccgttac agctgatggc
    26821 ttccgcgtag gggcgacagc cgggataacc gcactgacca cactggctct gcggtaagat
    26881 ttcgtcaatt ttctcaacga ccggatcgtc ttccaccgca aaacggcggg aggcataacc
    26941 cagaatggcg ccaaacgcca ggcccagcag gctcacggcg gcaacggcaa tccagatagc
    27001 attcattaca acttcaccaa accactaaag cccataaagg ccagagacat aagacctgcg
    27061 gtaattaacg caatggcatt accgcgaaaa ggtgccggga catcagccac agcaaggcgt
    27121 tctcggatgg cggcgaagag caccatcacc agcgagaaac cgacagcggc ggaaaaaccg
    27181 tacagcgccg actgcaagaa attgtgcccg agattgatat tcagcaacgc cacgccgagc
    27241 actgcacagt tggtggtgat aagcggcaaa aaaatcccca gcagacggta aagcaccggg
    27301 ctggttttgc gcaccaccat ctcggtgaac tgcacgacca cagcaatcac cagaataaat
    27361 gccagggtgc gcaggtaaat cagattaagt gggatcaaaa tccacgtatc gataagccag
    27421 gcgcaaatag acgccagcgt catcacaaac gttgttgcca gccccatgcc catcgcggtt
    27481 tccagttttt tggaaacccc cataaacgga cagagaccga gaaacttgac cagtacaaag
    27541 ttattgacca gtacagttcc gacaaacagt aacaggtagt cagtcatttt tcggcctgaa
    27601 ataaaaaagc cgcctattat ctgttaatcc agagcaggcg acaacaggtt aactgtaagg
    27661 ttattacgga ttcacaaacg tcgcttttac ccgcgacgag cgtttgaaat aggggacgat
    27721 caatgcggtt gccaacaaag taaacagcaa ctggcgaacg gctatgccaa cttccacggg
    27781 tgaaaaagcg aatgctttta ctgccagcaa tacggaaata agcagccaga taatatagtg
    27841 tttaggaaca caacgccgac gtttaaagaa agcgatggtc aaccacagtg tgtaatacca
    27901 catggcgata gcggtaataa aagaaacgcc ccataagatt tgtgtcgtca gtgcttgtcc
    27961 gccgagcgtt tgaaatgttt gcggagaaga taacgcagcg gtatacagca acaacgccag
    28021 cgtcgtactc aacagagcga ctaaaagcca ggccaacggg ccaagcaacc agcctccaat
    28081 acgttgtggc gttgtggtgg tcataggttc tccagaatag cgacgcaaac aggcaaatct
    28141 aattttaggg gcgttattat aaggcttttg taaccgatcg ctactttctt tattggacat
    28201 agtgccagac ggacttcggt acctggccga ggtcaaacaa acgcccgcca gaaaccagct
    28261 ctgcgcgacg gtgatcggca gcacgataca tattgatcag ttcctgatcg tccgtcaggg
    28321 tgtaattaag atggtcgtag agtttttcca gactttcgag tgaactgatt ttgcgaaatt
    28381 ttaataagta gtcctgaaca gtcataaaag gtccataaaa taattaatac caaaggggta
    28441 ggtattatta gtgatgataa aatttcacca aaagtgaacg cttgtgttat tacaataata
    28501 agaatgcgct gatggcaaat attcagggat gaaaaactgc tctgaaaaga gtaacgcgct
    28561 ttactatccc tgacaatcac caacaacatc gaacaagata ataaattcct ggtttaatat
    28621 ccgacaagtg aaaacatgca cccggacggg cagcatgtcg ctccacaagt gcagagctta
    28681 cttgtgttgt accgaagcac tctgttcagg tggctgatag ttgtcaatgt gactcgccac
    28741 gccaagaaga atgactgaaa cgacaagaac gatccaacct gttaattcaa taagacgatt
    28801 cattacagcc cacatctctc ttgattgatc cattaacttc agggggtaaa tgttacttag
    28861 caataatagc tccagcaaga tttttactga ggtttttgcg atatttagct tttgtcgttg
    28921 gaaaattcgc tatttttttg acttaagtta aacaacatcc cttattgctg gcaggttatt
    28981 aaactgttga gcgtgggtaa aggatagtgt caaatagcca tcatacttca atgagaggca
    29041 atgacatgag cgacaacatc cgtgttgggt tgattgggta tggttatgcg agcaaaacct
    29101 tccatgcgcc cctgattgcg ggcacgcccg ggctggaact ggcggtaatc tccagcagtg
    29161 atgaaacaaa agtaaaagcc gactggccaa cggttacggt tgtctctgag ccgaagcatc
    29221 tgtttaacga tcccaacata gacctgattg tcattcctac acccaacgat acccatttcc
    29281 cgttagccaa agcggcgctt gaggcgggta aacatgtggt cgttgataaa ccctttaccg
    29341 tgacactgtc acaagcgcga gagctggatg cgctggcaaa aagcctgggg cgtgtgctgt
    29401 ctgtattcca taaccgtcgt tgggatagcg atttcttgac gctaaaaggt ttgctcgcgg
    29461 aaggcgtgct gggtgaagtt gcttactttg agtctcattt tgaccgcttc cgtccgcagg
    29521 tgcgcgatcg ttggcgtgaa cagggcggcc caggcagcgg tatctggtac gatttagcac
    29581 cgcatcttct tgatcaggcc attacgctat ttggtttacc ggtcagcatg acggtagatt
    29641 tggcacagtt acggcccgga gcgcagtcga ccgattattt ccacgccatc ttgtcctatc
    29701 cacagcggcg agtcatttta cacggtacca tgctggcagc cgctgagtca gcacggtata
    29761 tcgtgcatgg atcccgaggc agttatgtga aatatggcct cgatccacag gaagaacgtc
    29821 tgaaaaatgg cgagcgtctg ccgcaggaag actggggcta cgatatgcgt gatggcgtac
    29881 ttacccgcgt ggaaggtgag gaacgtgtcg aagaaacgct gttgacggtg cctgggaatt
    29941 atccggctta ctatgcggcg attcgtgatg cgttaaatgg cgatggtgaa aatccggttc
    30001 cggcaagcca ggcaatccag gtaatggagt tgattgagct gggcatcgaa tccgccaaac
    30061 atcgcgcgac gttgtgcctt gcgtgaaaaa tggatggtgc aatttgcacc atccattttt
    30121 gttacttcgc ggcgactttt tctcgcagtg cgcgtttttc ctctgcgctg aggaaagcca
    30181 tttccagacc attaatctgt gcctggcgga tttgctcacg ggataaccca gcagctggcg
    30241 cggcaacggt atattcgtga atgatatcca ctccctgtac gccgggatcg tcagtgttaa
    30301 tgctggcacg aatgccatgc tcaaggaacg ttttcagcgg atgtgcagcc agctctgcta
    30361 cggtgctggt ctgaatattg gaggtcagac aggattcaat accaatttgt tgctcggcga
    30421 gaaaatccat cagcgcccga tcttcaatgg cttttacgcc atgtccaata cgctccgcac
    30481 ccagttcacg aatcgcctgc cagatgcttt ccggcccggc agcttcgcct gcatggacgg
    30541 taatatgcca gcccgcatca cgcgcgcggt tgaagtgaga aaggaacaga cttcccggga
    30601 atccaagttc atcaccagct aaatcaagtg cggtaatctg gtcacggtgg gctaaaaagg
    30661 cctccagctc ttgctgacag gcggcttcgc cgaaggttcg gctcataatg ccgataagct
    30721 tcgcctgcac accaaaggtg cggcaacctt cacgtacgcc atcgatcacc gcttcgacaa
    30781 cacccgctac aggcagctta tgtgccattg ccatgtagcc tggtgaaaaa cgcagctcga
    30841 catagtgcag gccgttacgg gctgcatctt caatgttttc aaatgccacg cggcggcagg
    30901 catcaagaga ggcgagaact ttaacgcccc agtcaagttt ggtcagaaag ctcaccagat
    30961 cgggttcgtt ggcaatgacc tgaacgtggg gaatcagtgt ttcaagggat tgtgcaggaa
    31021 gcgagatatt atactggcgg ccaagttcaa gaatggtctg gggacgaatg ttgccatcaa
    31081 ggtggcgatg gatatcagtt aatggcaggg tggtatcaat catggtcgca ctctttttct
    31141 ggatataaaa gtgcgcacca ttataaaaac aaagcagggt aaaaatccac gattgcgcaa
    31201 cgatttttta cgttgcgcaa ttggggttag cgaacagcgc ggatggcgtt tattaatcca
    31261 gccacacctt tttccagttt cgaacgtggg cagccggcat tgagacggac aaaaccacga
    31321 ccttcttcac cataggtata ccccggcatg atcgcgactt tttcttgctc gataagcgct
    31381 ttttgcaacg cgttgtcgtc aatattcaac ggacgtagat caagccaggc cagataagtg
    31441 gattgcggga tctgccagtt gagttcagga aacgcggcgt tcattttatc tgagatatac
    31501 gtcaggttat ctttcagata gacgcgtaag gcatccagcc acggtgcacc ttgctgatag
    31561 gcggcgatat gggcagttaa cgccagtacc gaaggggaag aaagcccatc acgtcctttc
    31621 agtgccgata aataggcatc acggctgctg ctattttcta taatcccgta agcaccggtc
    31681 agcgcgggaa tattgaaact tttcgatccc gacgtcagca acgcccagtc tccccgcgcc
    31741 acattactcc agggaatatg cggctgctcg ccccagacca tatccatatg gatttcatcg
    31801 gaaataaccc gcacatcatg acgctcgcac aggtcagcca tgatctccag ctcatcgcac
    31861 gtccacactt tcccggtagg attctgtggg ctacacagga gcataatttt acattctggt
    31921 ttcgccaaaa cggcttccag cttgcccata tcgcaaaacc agccgtcagc ctgcttctct
    31981 aaagcaacgg gcattactgt gcgctggtta ccttcaatgg ccttgtaaaa tgcgtcatag
    32041 gcgggcgtgt ggatcaccac gccttcacct gtttcagacc actgacgaat cagttctgaa
    32101 accatataga tgacagaagg gccatacacc accgtctgcg tatcaattgc ggtgtaatgc
    32161 tgggtggaaa accagtgggc aatagccgcg agaaactcat cgtttttcca gcggctgtag
    32221 ccaaatacgc cgtgcatcag gcgctgattc agcgcctcga taatgcaggg ggcagtggca
    32281 aaatccatgt ctgaaatcgt gaacggtaac aggtcagcag tgccgaaacg gtcagcgaca
    32341 taatcccact gtgtacacca tgtgccatga cgatccacga cctttgaaaa atcgaacata
    32401 tcttatcctt atgcctggac agtatgcatc agaccggcca tttcatcttt tactgactga
    32461 acttgtggcc cgataacaac ctgcaggtta tgttgattaa gttgtactac gccaattgcc
    32521 cgattgtcct tcagtgcctg cacattaaca agcgacatat cttccacaga caaacgcaga
    32581 cgggtaatgc agttatcgag gctgacaata ttgtcggcac cgcctaatgc ttcgaggatt
    32641 gcaggaacgt tgtaacctga tttacccggc gcactggcaa cggctttttc gattgagctg
    32701 gcaacttcgc tatcgcgccc cggggttttc agattgaagc gggtgatagc gaaacggaag
    32761 atgacgtagt aaacgacaaa ccagattgcc gccaccactg gcaccatgta ccacttggtt
    32821 gacagaccat gcaaaatacc gaacaccacg aagtcgatga tattgccgtc ggtattaccg
    32881 atggtgacgc cgagcacaga catgacggtg aagccgaggc cggttaacag cgcgtggatg
    32941 acatacagaa ctggcgctac gaacaggaac aggaattcca gcggttcggt agtgccgcca
    33001 acgacgcagg cgatcaggcc agaaatcagc agacctttaa ttttatggcg attttccggg
    33061 cgcgcgcagt gatacatagc taacgctgca cctggcagac cgccgagaaa cgcaggcatt
    33121 ttaccttgcg aaaggaaacg cgtgacgctt tcagaaaaac cgtgagtggt cgggcaactc
    33181 aattgcgcct ggaagatggt cagtgcgccg ctgacggttt gaccgcagac ttcctgcgtg
    33241 ccgcctgcgt cggtaaagcg aattaatgcc accagaatgt gatgcagacc aaacggcaac
    33301 agcagacgtt caccggtacc aaacagcatc ggtccgaaat cacccgcgct gtttatcata
    33361 tggcccaagc cgctaatacc catggcgaaa atcggccaga ctaatggaat caccaggccg
    33421 acaaggccca tcaccagcga ggagataatt ggtacgaagc gcgtaccgcc gaagaatgcc
    33481 agcgcatccg gcaggcggat attatggaaa cgctcatgca gcatccagac gataataccg
    33541 gcgatcaccg caccgaggat cccggtatcg atcgactgga tcccaagaat gctctggatg
    33601 ttattggctt tcagaacggc ggcatctgtg gttggcagaa tgcctttatt ggtcaaccag
    33661 aagtttaccg cgaggttcat taccgcataa ccgacgaagc cagcgaatgc cgctacgcct
    33721 ttattttcgc gtgccaggcc cagcgggatg gcgatacaga acatgacagg caggaaacta
    33781 aaagcaaacg agccaatctt actcatccag gtaaagatag cctgcaacac ggggttgccc
    33841 aggaccggga tcagggttat gacatcatgg ctgctaagag aactaccaat gccgagcata
    33901 atgccgcaga acgacaataa tgccacgggt aacatgaagg ttttgcctaa ctgctggaag
    33961 aactcccaca gcgtgacttt cggtgctgtt ttcgccgtca taaaacgact cctcgtagag
    34021 aacaatctgg attcagtaaa ttgcgcgaga agataaaacg ttttatcaaa ttttagtgag
    34081 tcataaatca cattacgcaa cgataatagc gggtataaga taaataaaag gtaaaacgtt
    34141 ttatctgtca cataatcagg gagtaggtca tctgcatggc taccgccaaa aaaataacca
    34201 ttcatgatgt tgcgctggct gcgggcgtgt cggtaagtac cgtttcgctg gtgcttagtg
    34261 gcaaagggcg aatctctacc gccacaggag tacgcgttaa cgccgccatt gaagagctgg
    34321 gatttgtgcg caatcgccag gcgtcggcgc tgcgcggcgg gcaaagcggc gtcattggtt
    34381 tgatcgtccg tgatttatct gcgccgtttt acgccgaatt gacggccgga ttgacggaag
    34441 ctctggaagc gcagggacgg atggtttttt tgcttcacgg cggtaaagac ggcgagcagc
    34501 tggcacagcg gttttcactg ttactgaatc agggtgtcga tggtgtggta attgccgggg
    34561 ctgcaggaag cagcgatgac ctgcgacgga tggcagaaga aaaagctatc ccggtgattt
    34621 tcgcttcccg tgccagttat cttgatgatg ttgatacggt tcgcccggac aacatgcagg
    34681 ctgcacagtt gttgacggag catctcattc gcaatgggca tcagcggatc gcctggctgg
    34741 gagggcaaag ttcctcatta acccgtgcag aacgggtggg gggctattgt gcaactctac
    34801 taaaatttgg cctgccgttt cacagcgatt gggtgttgga gtgcacttcc agccagaagc
    34861 aagccgcgga agctatcacg gcgcttttac gtcataaccc gaccatcagt gccgtggttt
    34921 gctataacga aactattgcg atgggggcat ggtttggttt gctgaaagca gggcggcaaa
    34981 gcggggaaag cggagtcgat cgttactttg agcaacaggt ttcgctggcg gcatttaccg
    35041 atgcgacacc agccacactt gatgatatac ctgttacctg ggtcagcacg ccagcgcggg
    35101 aacttggtat cacacttgcg gatcgcatga tgcaaaaaat cacccatgaa gagacgcatt
    35161 cacgcaatct tattattccc gcccggctca ttgcggcgaa ataatcctct ctttatctgc
    35221 tatacttggt agtgtccttt cctcaaggtt aatgataaaa aatgtggcac acaggcgg
//
LOCUS       DADLSA010000027        35156 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.027, whole genome shotgun sequence.
ACCESSION   DADLSA010000027 DADLSA010000000
VERSION     DADLSA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.027"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..1587
                     /locus_tag="JN691_001332"
     CDS             <1..1587
                     /locus_tag="JN691_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY7242820.1"
                     /translation="LQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDF
                     AAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGAIS
                     VDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWAN
                     NKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSG
                     SSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQIY
                     MSNSTTFDDQGFASNNTGLSGPVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVAT
                     VNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYR
                     TTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQR
                     KPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQG
                     LSCTITFGKEIDESRNYICQ"
     gene            1603..2636
                     /locus_tag="JN691_001333"
                     /pseudo
     CDS             1603..2636
                     /locus_tag="JN691_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(2718..3056)
                     /gene="rcnB"
                     /locus_tag="JN691_001334"
     CDS             complement(2718..3056)
                     /gene="rcnB"
                     /locus_tag="JN691_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HAY7242821.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3275..4123)
                     /locus_tag="JN691_001335"
     CDS             complement(3275..4123)
                     /locus_tag="JN691_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HAY7242822.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHDHEHHQDHEHHHDQGHHHHHEHGEYQDA
                     HARDHANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLV
                     VSFSIGLALTLFTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHG
                     FMGIMR"
     gene            4245..4517
                     /gene="rcnR"
                     /locus_tag="JN691_001336"
     CDS             4245..4517
                     /gene="rcnR"
                     /locus_tag="JN691_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HAY7242823.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      4609..4705
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4740..5529
                     /gene="thiM"
                     /locus_tag="JN691_001337"
                     /pseudo
     CDS             4740..5529
                     /gene="thiM"
                     /locus_tag="JN691_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
     gene            5526..6326
                     /gene="thiD"
                     /locus_tag="JN691_001338"
     CDS             5526..6326
                     /gene="thiD"
                     /locus_tag="JN691_001338"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAY7242824.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6391..7209
                     /locus_tag="JN691_001339"
     CDS             6391..7209
                     /locus_tag="JN691_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HAY7242825.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            7261..8007
                     /locus_tag="JN691_001340"
     CDS             7261..8007
                     /locus_tag="JN691_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7242826.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(7981..8946)
                     /locus_tag="JN691_001341"
     CDS             complement(7981..8946)
                     /locus_tag="JN691_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7242827.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(8943..9946)
                     /locus_tag="JN691_001342"
                     /pseudo
     CDS             complement(8943..9946)
                     /locus_tag="JN691_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(9943..11220)
                     /locus_tag="JN691_001343"
     CDS             complement(9943..11220)
                     /locus_tag="JN691_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7242828.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPNTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYTYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11477..12529
                     /gene="fbaB"
                     /locus_tag="JN691_001344"
     CDS             11477..12529
                     /gene="fbaB"
                     /locus_tag="JN691_001344"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAY7242829.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            12837..13684
                     /gene="gatY"
                     /locus_tag="JN691_001345"
                     /pseudo
     CDS             12837..13684
                     /gene="gatY"
                     /locus_tag="JN691_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
     gene            13713..14975
                     /gene="gatZ"
                     /locus_tag="JN691_001346"
                     /pseudo
     CDS             13713..14975
                     /gene="gatZ"
                     /locus_tag="JN691_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            14985..15437
                     /gene="gatA"
                     /locus_tag="JN691_001347"
     CDS             14985..15437
                     /gene="gatA"
                     /locus_tag="JN691_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAY7242830.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            15468..15752
                     /gene="gatB"
                     /locus_tag="JN691_001348"
     CDS             15468..15752
                     /gene="gatB"
                     /locus_tag="JN691_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAY7242831.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            15756..17111
                     /locus_tag="JN691_001349"
     CDS             15756..17111
                     /locus_tag="JN691_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAY7242832.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            17177..18217
                     /gene="gatD"
                     /locus_tag="JN691_001350"
     CDS             17177..18217
                     /gene="gatD"
                     /locus_tag="JN691_001350"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY7242833.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            18317..19096
                     /locus_tag="JN691_001351"
     CDS             18317..19096
                     /locus_tag="JN691_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7242834.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(19178..20077)
                     /gene="yegS"
                     /locus_tag="JN691_001352"
     CDS             complement(19178..20077)
                     /gene="yegS"
                     /locus_tag="JN691_001352"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HAY7242835.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMPTGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            20483..20800
                     /locus_tag="JN691_001353"
     CDS             20483..20800
                     /locus_tag="JN691_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242836.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(21142..22503)
                     /locus_tag="JN691_001354"
     CDS             complement(21142..22503)
                     /locus_tag="JN691_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAY7242837.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(22650..22982)
                     /locus_tag="JN691_001355"
     CDS             complement(22650..22982)
                     /locus_tag="JN691_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAY7242838.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            complement(23173..23895)
                     /gene="baeR"
                     /locus_tag="JN691_001356"
     CDS             complement(23173..23895)
                     /gene="baeR"
                     /locus_tag="JN691_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAY7242839.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAVSYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(23892..25295)
                     /gene="baeS"
                     /locus_tag="JN691_001357"
     CDS             complement(23892..25295)
                     /gene="baeS"
                     /locus_tag="JN691_001357"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAY7242840.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(25292..26707)
                     /locus_tag="JN691_001358"
     CDS             complement(25292..26707)
                     /locus_tag="JN691_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7242841.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(26708..29785)
                     /gene="mdtC"
                     /locus_tag="JN691_001359"
     CDS             complement(26708..29785)
                     /gene="mdtC"
                     /locus_tag="JN691_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HAY7242842.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTEQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(29786..32908)
                     /gene="mdtB"
                     /locus_tag="JN691_001360"
     CDS             complement(29786..32908)
                     /gene="mdtB"
                     /locus_tag="JN691_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HAY7242843.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(32908..34155)
                     /gene="mdtA"
                     /locus_tag="JN691_001361"
     CDS             complement(32908..34155)
                     /gene="mdtA"
                     /locus_tag="JN691_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HAY7242844.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            34434..34490
                     /locus_tag="JN691_001362"
     CDS             34434..34490
                     /locus_tag="JN691_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7242845.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            34762..34818
                     /locus_tag="JN691_001363"
     CDS             34762..34818
                     /locus_tag="JN691_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7242846.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            35090..35149
                     /gene="ibsA"
                     /locus_tag="JN691_001364"
     CDS             35090..35149
                     /gene="ibsA"
                     /locus_tag="JN691_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HAY7242847.1"
                     /translation="MMKHVIILVILLVISFQAY"
BASE COUNT         8766 a         8780 c         9223 g         8387 t
ORIGIN      
        1 ttgcagttgg ccggtggtgg agcagatctt gatgttagcg tgaaagaggc ggacggctcg
       61 gtaaccacct atctggtgcc ttatgcagcg gtgccaaata tgctgcaacc cggcgtgtcg
      121 aaatatgatt ttgcggcggg ccgtagccat attgaagggg cgagcaagca aagtgatttt
      181 gtccaggcag gttatcagta tggttttaat aacttattga cgctgtatgg cggcacgatg
      241 gttgctaata attactatgc gtttaccctc ggaacgggtt ggaacacgcg cattggcgca
      301 atttccgtcg atgccacgaa gtcgcatagt aaacaagaca acggcgatgt gtttgacggg
      361 caaagttatc aaattgccta caacaaattt gtgagccaaa cgtcgacgcg ttttggtctg
      421 gcggcctggc gttattcgtc gcgtgattac cggacattta acgatcacgt ttgggcaaac
      481 aataaagata attatcgccg tgatgaaaac gatatctatg acattgccga ttattaccag
      541 aacgattttg gccgtaaaaa tagcttttct gccaatatga gccagtcatt gccagaaggc
      601 tggggttctg tgtccttaag tacgttatgg cgagattact gggggcgtag cggcagcagt
      661 aaggattatc agttgagtta ttccaataac tggcggcgga taagctatac cctcgcggca
      721 agccaggcgt atggcgagaa tcatcatgaa gagaaacgtt ttaatatttt tatatcaatt
      781 ccctgtgatt ggggtgatga cgttacgacg cctcgtcggc aaatatatat gtctaactca
      841 acgacgtttg atgatcaggg ttttgcctca aataatacgg gattatcagg accagtaggg
      901 agtcgggatc agttcaatta tggtgtcaac ctgagtcatc aacatcaggg aaatgaaacg
      961 acagctgggg cgaatttgac ctggaacgcg ccggttgcga cagtgaatgg cagttatagt
     1021 cagtcgagta cttatcgaca gactggagcc agtgtttcag ggggcattgt cgcctggtcg
     1081 ggtggcgtta atctggcgaa ccgtctttcc gaaacgtttg ctgtgatgaa tgcgccagga
     1141 attaaagatg cttatgtcaa tgggcaaaaa tatcgcacaa caaaccgtaa tggagtggtg
     1201 gtatacgacg gaatgacacc ttatcgggaa aatcacctga tgctggatgt gtcgcaaagc
     1261 gatagcgaag cagaattacg tggcaaccgg aaaattgccg ccccttatcg cggcgcggtt
     1321 gtactggtta attttgatac cgatcagcgc aagccctggt ttataaaagc gttaagagcg
     1381 gatggtcaac cattaacgtt tggttatgaa gtcaatgata tccatggtca taatattggc
     1441 gttgtcggcc agggaagcca gttatttatt cgcaccaatg aaataccgcc atcggttaat
     1501 gtagcaattg ataagcaaca aggactttca tgcacaatca ccttcggtaa agagattgat
     1561 gaaagtagaa attatatttg ccagtaatag tcaggtggtt ctatggaaat tcgcataatg
     1621 ctatttatat taatgatgat ggttatgcct gtaagctatg cggcatgtta tagtgagtta
     1681 tctgttcagc acaatttggt tgttcagggg aattttgcac ttactcaaac acaaatagcg
     1741 acatatgagc ataattttaa tgattcgtca tgcgtgagta caaatactat cacccctatg
     1801 agcccatcgg atattattgt tggactttat aacgatacca ttaaattaaa tttacatttt
     1861 gaatggacca ataaaaacaa catcacgttg tcaaataatc agagcagttt caccagtggt
     1921 tattcggtaa cggtgacacc tgcggccagt aatgcaaaag tgaatatttc tgcggggggc
     1981 ggtggttcag tgatgattaa tggtgttgcg acattatcca gtgcttcatc atcgacacgc
     2041 gggagtgccg cagtacaatt tctactgtgt ttattaggtg gcaagtcatg ggatgcatgt
     2101 gtaaatagct acagaaatgc attggcacaa aatgcaggtg tctattcctt caatctgaca
     2161 ttgtcataca acccgataac cacaacctgc aaacaggacg atttattaat tactttagac
     2221 agtattcccg tttcacaatt accagccaca ggtaacaaag caacaataaa tagtaaaaaa
     2281 agggatatta ttctgcgttg taaaaattta ttaggtcaac aaaatcaaac atcacggaaa
     2341 atgcaggtgt atttatcaag ttctgacttg ttaaccaaca gcaacacaat actgaaaggt
     2401 gcggaagata atgcgtagga tttattcttg aaagtaatgg ttcgccagtc acacttttaa
     2461 atatcactaa cagcagtaaa ggatacacaa atttaaaaga aattgcggca aagtcaaaac
     2521 ttacagatac aacggtttca attccgataa cagccagtta ctacgtctac gatacaaaca
     2581 aagttaaatc tggcgcactg gaggcaaccg cattaatcaa cgtgaaatac gactaataac
     2641 aagcaagacg agcaagtggc taataataaa aaataacaag gtgtctgcat ttccccctca
     2701 tgatgagggg gcttttttta gcgatgataa aaaatctcac cgtcgtaggc tttaatgatt
     2761 ttaccgtcgg tgtcgctgat caacacgtac gcgccaccca tataggtcca gtgcgtcccg
     2821 gcatcaggcg cgggcaggtt acgtaactgc cactgcttaa tgttgtactc atcggtgcgg
     2881 tacatctctg ggacggtatc acctggtttg aaatgcttaa agtcagcaat gaaactactt
     2941 aactcatact tagaaattcc cgaggtattg gtcgaacccg cggtggctgg tgcggcccag
     3001 gcggcactgg taaccagcaa aagcacaccc agcaacatct tatttttaat ggtcataaac
     3061 tctccacttt ggggatctgt aatatcagtc gcatatcttt cctgccatac aatataactg
     3121 acaagcgata ttttgttagt gatcccatga tttcgtaaaa agtgtaaatg aaaggatgcc
     3181 ctaaaagcat ccttagcgag cagagagtca gcggaaatag ctgatttaag aatcatcctg
     3241 ggctgaagat aacatgcaag cctaaaggat tgagttatcg cattatgccc atgaagccat
     3301 gtacgcccat atacacaccg actaagccaa tcaacagact ggaaaaatag ggggcgcgtt
     3361 tagcgagagt gttaaatccg ctccagcgtt ttgcgacctg ctgaacgctg attgctgcgc
     3421 caacgcctac ggtgaaaagc gttaacgcca ggccaatgct gaaactgacg accagtgttg
     3481 cgcccagtgt cagggctttc aactgaatgc atatcaacag cacggtaatt gctgccgggc
     3541 aggggataag gccaccggtt aagccaaata acaaaatttg ccagttgatg acctctctac
     3601 catcaaagcg tcgtttaatg tcattggcat ggtctcgtgc atgggcatcc tgatactcgc
     3661 catgttcgtg atggtgatga tgtccttggt cgtggtgatg ttcgtggtct tgatgatgtt
     3721 cgtgatcgtg atgatggtga tgatgctcat aatcatgccc gtgcatattc tccagccagt
     3781 tgcgttcgcc gcgccaggta cgccagaaca tccagaacgc ggtgccaata atgatcactg
     3841 cggaaatcag ctggagccac ggttctgctg attgagcagt aaagcgctta ctgatcacca
     3901 tcccgccaaa ggcaatcaac cagaccaccg cggtatgcga aatggttgct gccagtccga
     3961 gcatcaccgc ctgtttaatg gtgcctttga tggcgatgat aaacgccgcc atcatcgttt
     4021 ttgagtgccc tggttccagg ccatgaagcg caccaagtaa gatggcgctg gggatgaaga
     4081 accaggcgtt tccttgctga agaagagttg taaattcggt catgataata attcttagta
     4141 ttaattcggc aatctgattc tactcccccc cagtacctga tactactccc ccagtagatt
     4201 aatagtgcta tgatttttca tattcttgtt aaccaggtgt tgccatgtct catacaatcc
     4261 gtgataaaca gaaactgaaa gcgcgtgcca gtaagattca gggccaggtc gtggcgctca
     4321 agaaaatgct cgacgagccg cacgaatgcg ctgcagtttt acaacagatt gctgctatcc
     4381 gtggagcggt aaacggtctg atgcgggaag tgattaaagg tcatctgacg gaacacatcg
     4441 ttcaccaggg ggatgagcta aaacgtgaag aagatctgga tgtcgttctg aaggtgctgg
     4501 attcatatat caaataattt attaacgcga ttgtaaaact gccgtttttc ctcgtttaca
     4561 acgggtgcgc tggacattac catcctcctc tgcgatttat catcgcaacc aaacgactcg
     4621 gggtgccctt ctgcgtgaag gctgagaaat acccgtatca cctgatctgg ataatgccag
     4681 cgtagggaag tcacggacca ccaggtcatt gcttcttcac gttatggcag gagcaaacta
     4741 tgcaagtcga cctgctgagt tcagcgcaat ctgcgcacgc gttacacctt tttcaccaac
     4801 attcccctct tgtgcactgc atgaccaatg atgtggtgca aacctttacc gccaatacct
     4861 tgctggcgct cggtgcatcg ccagcgatgg ttatcgaaac cgaagaggcc agtcagtttg
     4921 cggctatcgc cagtgccttg ttgattaacg ttggcacact gacgcagcca cgcgcacagg
     4981 cgatgcgtgc tgccgttgag caagcaaaaa gctctcaaac acgctggacg cttgatccag
     5041 tagcggttgg tgcgctcgat tatcgccgcc atttttgtca tgaactttta tcttttaaaa
     5101 cggcagcgat acgtggtaat gcttcggaaa tcatggcatt agctggcatt gctaatggcg
     5161 gacggggagt ggataccact gacgccgcag ctaacgcgat acccgctgca caaacactgg
     5221 cacgggaaac tggcgcaata cgtcgtggtc actggcgagg tggattatgt taccgatgga
     5281 catcgtatca ttggtattca cggtggcgat ccgttaatga ccaaagtggt aggaactggc
     5341 tgtgcattat cggcggttgt cgctgcctgc tgtgcgttac caggcgatac gctggaaaat
     5401 gtcgcatctg cctgtcactg gatgaaacaa gccggagaac gcgcagtcgc cagaagcgag
     5461 gggccaggca gttttgttcc acatttcctt gatgcgctct ggcaattgac gcaggaggtg
     5521 caggcatgaa acgaattaac gctctgacga ttgccggtac tgatccgagt ggtggtgcgg
     5581 ggattcaggc cgatcttaaa accttctcgg cacttggcgc ttatggttgc tcagttatta
     5641 ctgcactggt ggcgcaaaat acccgtggcg tacagtcggt gtatcgcatt gagcctgatt
     5701 ttgtcgccgc ccagctcgat tcggtgttca gcgatgtgcg aatcgatacc actaaaatcg
     5761 gtatgttggc ggaaaccgat attgttgaag cggtggcaga acggttgcaa cgttatcaga
     5821 tccaaaacgt ggtactcgac accgttatgc tggcaaaaag cggcgacccg ctgctttcac
     5881 cttcggcggt tgctacgctg cgcagtcgat tattgccaca ggtttcatta ataacgccaa
     5941 acttgcccga agctgccgcc ttgctcgacg cgccacacgc gcgcaccgaa caggaaatgc
     6001 tggaacaagg gcgatcgctg ttggcgatgg gctgtggcgc agtgctaatg aaaggtggtc
     6061 atctggatga tgagcaaagc ccggactggc tgtttacccg cgagggtgaa caacggttta
     6121 ccgcaccgcg cattatgacc aaaaacaccc acggcactgg ttgtacactc tctgcggcgt
     6181 tggctgcact acgcccgcgc catacaaact gggctgacac cgtacaggag gcaaaaagct
     6241 ggctttcatc ggcgttagcc caggccgaca cgctggaagt tggtcacggt attggtccgg
     6301 ttcaccactt ccacgcctgg tggtgagcac cgtactatag ctgctttggt tattgtcagc
     6361 ctttggcact gacattcagg gatgaaacca atgcagctaa gaattaccag tcgaaaaaaa
     6421 ctcaccgctc tattatgcgc tcttgggcta atttccatcg ttgcaattta tccgcgtcag
     6481 acggtgaatt ttttctattc gacagcagtt cagattacag actacatcca cttctacggt
     6541 tatcgcccgg ttaaatcttt cgctattcgc attcctgcca gttacaccat tcacggatta
     6601 gatgtttcgc gctggcagga gcgtatcgac tggcagcggg tggcaaaaat gcgcgacaac
     6661 gatatccgct tacagtttgc ttttattaag gctacggaag gcgaaaagct ggtggacccc
     6721 tatttttcgc gtaactggca attaagccgc gaaaatggcc tgctgcgcgg ggcgtatcat
     6781 tatttctccc cgtcggtatc cgcttcagtt caggcgcgat tatttctgca aacggtggat
     6841 ttctcacaag gcgatttccc tgccgtgctg gacgtagaag aacggggaaa attatcggca
     6901 aaagaattac gcaagcgggt aagtcagtgg ctaaaaatgg tcgaaaaacg cacgggaaaa
     6961 aagccgatta tttactcagg agccgttttt tatcacacca atctggcggg ctatttcaat
     7021 gaatatccgt ggtgggtggc tcactattat caacgtcgtc cggacaatga cggcatggcc
     7081 tggcgcttct ggcagcattc cgaccgtgga caggtagatg gcatcaatgg tccggtggat
     7141 tttaatgtgt ttaatggcac ggtggatgag ttgcaggcat tcgttgatgg gattaaagaa
     7201 acgccttaaa taagaagcag tgatatactc ccaccagact ccctggcagg aaaaagacaa
     7261 atggaacaag cgcataccca gctgattgcc caactgaacg agcgtatttt agcggcggat
     7321 aacacgccgc tttatattaa gtttgccgaa acggtaaaaa atgccgtgcg cagcggggtg
     7381 ctggagcatg gcaatatttt gcccggtgag cgtgatttaa gtcagttaac cggcgtttcg
     7441 cgcattacgg tgcgcaaggc gatgcaggcg ctggaagaag agggcgtggt gacgcgttcg
     7501 cgcggttacg gcacgcagat caacaacatc ttcgaatact cgttgaaaga agcgcgaggt
     7561 ttttctcagc aggtggtatt gcgcgggaaa aagcccgata cgctatgggt taataagcgt
     7621 gtcgtgaaat gtcccgaaga agtcgcgcag cagctggcgg tcgaagcggg aagtgatgtc
     7681 tttttgctta agcgtattcg ctatgtcgat gaagaagcgg tatcgattga ggaatcgtgg
     7741 gttccggcgc atttaattca tgatgttgat gccatcggaa tttctcttta tgactatttc
     7801 cgcagccagc acatttaccc acaacgtacg cgttcccgcg ttagcgcccg gatgccggat
     7861 gccgagtttc agtcacatat tcagttagat agcaaaatac cggtgctggt gatcaagcaa
     7921 gtggcgcttg accaacagca acggcctatt gagtacagca ttagccactg tcgcagcgat
     7981 ctatacgttt ttgtgtgcga ggagtagttc ctcgcgcgtt ggcgcacaat caccgccccg
     8041 atgcccgaca acccacgacg ccactgcatt gcccagtaat acggcatccg ccagtggcag
     8101 accagaggcc agcccggcaa gtacgccacc tgcatgactg tcgcccgccc caatggtgtc
     8161 tacaacttgc gttggaaatg ccggaatgca gccagaagcg tcgttgctga aataccatgc
     8221 gccttcttta tcgagacgaa cgatcaacgg cgcggcaaat ttctcctgcc attgcttgcc
     8281 aagtgttgtt atctctgcgg ataaagcaaa acgttcggcg gcaatctcag cctcttgacg
     8341 attgagtgac actaaaggtc gacaggccat gatccgcgcc agtaatgcgt ccgggatatc
     8401 gccaatacgt gggccaaaat cgataaacgg cgtcacgtct tgcagctttt ccagccattc
     8461 cactaacaat tcgccgcagg gcgaggccag ttgataaccg gaaaaataga gcaggttgcc
     8521 aggcgcaacg gttaatcgtg ccaaccattg gcgattccat tgattttcca caccgctgaa
     8581 tgacataaaa gtgcgttcgc catccggctc aaccagtgcc agacaccagc cgttatcgct
     8641 ttcggcgtta tcgatcaggc tgattaaccc ctctttcgcc atccggttgc gaataatctc
     8701 cgcccacacg ccctgaccga gcggcaaggc attacccgct tcgatgccga ggcgctttaa
     8761 cgccacggca atattcagtg cgcagccgcc aacgttaacg ctctgctgtt tcagttcgat
     8821 atcgcaccca cgccagggga gggcataagc gtcggcgatc acgtcaatga ccgccgcgcc
     8881 gacgaccatc accggctgac gcgtggttaa ttcaggcagc agtgtgtgta atcgagcgcc
     8941 gctcataccg cctcccgttg ttgacgatat ttcgccagcg ctgtggcata gcggttgaaa
     9001 tcaagctgat ttaccgcatc cagctccgcc tttaacgcag gatcgacagc gttaatgcca
     9061 tgcaacgcgc cgcaaatcgc cgtcgccata gcacctatgg tgtctgtgtc gccgccaaga
     9121 ttggcgcaca ggacggcgca gcggtttggg tcggtttgtg ccagttcaac tagcgcaatg
     9181 gcgcacggaa cggactcaat agtgctggtg cctgcgccaa cgacttggta aagctgttcg
     9241 ctggcggatt cggtgccgtc ggcattacgc acaattttca gcgcaatctc cagacgtgcc
     9301 gccagtgagg cgctgaatgt ggtggtgcgt ttttgctgtg catgtcgcgc aattgaaggc
     9361 aatgaatcaa caatcgctga ccagcgttct ccgtcaatgg cacgagaaat cgcccatgcg
     9421 atgactaccg cgcctgcaac cgccagatcg gatttatggg tcgggctgga cgccagcgcc
     9481 acatcatcaa taaaggaatc aacatcacgc gctggaagca aacaacctaa tggcgagacg
     9541 cgcatcgctg cgccgttggt tacgccgttg ttttccagtt cagcaacggg tttaccgtcg
     9601 cgaatggcgt taagcgcaat cttcgaggtc ggacctagta cgtttttgtt aaaggcgtcg
     9661 aaacgcaacg cccagtcgag aatattacgc ccaatcagat tcggatcgat cttgccttca
     9721 cattccagta acgcatccgc cagacacagg ccatcgaggt atcgtcggtg aattcggcgc
     9781 ggttaaaata acaggccgcg ttattctcct ttggtccagg cagaaaacgg tcaatccagc
     9841 caaagtgtgc tttaacgcgg ctgcgtggcc aaagctcgga gggcatcccc atcgcatccc
     9901 ctaacgcctg cccataaaga gcaccgagaa tacgttctgt tttcatttaa cttccccttg
     9961 tgtcaacgca atatctcgat catcgacctt gatagccgta atttcgttgt cggattcgcg
    10021 gaaaaaaatc atgaacagca cggcgataat ggcaatcatc accgcgccga aagtccacat
    10081 cccggaccag ttgaaagtca gtccgtttac cggttcctga taagcgaaca tcttttccat
    10141 catcacaccg ccaagacgat agccgagcaa actgccgaag ccttggcagc agagcgtgat
    10201 cagcccctgc gcagcggtac gcatatgcac gggggctttt ttatcgacat aggtgtaagc
    10261 ggtaacgtag taaaaatcgt aacttacgcc gtgaagcaaa ataccgagga acagtaacgc
    10321 gtaggtgaaa tattcatccg cactaccgta aataaagaag ccatagcaga tcgcagcggt
    10381 gaccagacca agcaataata cctttttgat accaaagcgt ttagtgaaaa acggcaatgc
    10441 cagcataaag aagatttcag agaactggcc gagcgtcatc cagccggtgg cgtttttcat
    10501 gccaacttcg gtcagataac cgttggcaaa gatgtaatag aacgccagtg gcatcgcaaa
    10561 caggaatgaa cagaagaaaa agacgaggaa gtttttatcg cgcagcagga tcagcgcatc
    10621 caggccgagc atgactttaa tgtccatttt gccggtgctt tttggcggcg tgttgggcag
    10681 gaaaaacgca aacacaccga gcagagcaga acttccggcg gtaattagca gcgggatgtt
    10741 agtcggtgag atatcggcat accccagcat ttgcggcaag aaaccacatg ccagaccgga
    10801 ggcaatccag ccgatagtgc ccatcacacg aatgcgtggg aaatcacgtt ccacatccgg
    10861 cacgttggca aaagcgatgc tgttagtcag cgcaatggtc ggcatatagg ttagcgagta
    10921 ggccagcagt aacggaaaga acccggcaaa agtggtctgt tgcgcagcga aatacatcag
    10981 cacagcacca gcgaacatca ataccgccag cactttctgc gccgagaaaa agcggtcagt
    11041 gatggagcca accagaattg gcgacaggat cgccgcaatg gctgtgcagg catacgacca
    11101 gccaatttct ccggcactaa aaccgctttt acttaaccac aaccacaatg gcacaaacca
    11161 cgcgccccag ataaaccatt caacaaacat catgaacgac agctttgctg ttgttttcat
    11221 cttttaattc cttcatgaca ggtaatgtaa ggtacgcgat gacaatacca cctaataatg
    11281 ccttttgaat acctttgcgg tgaaaacttg accgcgctaa cattttttct gatgaatcga
    11341 gccaacagaa aacgctgaaa aaacatccaa aagatggaaa aactcgcccg taacgaccag
    11401 gattatgatt gcccgcaaaa ggcgggccag gacaatccat agccgatatc caatcggaat
    11461 ttacgggagc atagtaatga cagatattgc gcagttgctt ggcaaagacg ccgacaacct
    11521 tttacagcac cgttgtatga ctattccttc tgaccagctt tatctccccg gacatgacta
    11581 cgtagaccgc gtgatgattg acaataatcg cccgccagcg gtgttacgta atatgcagac
    11641 gttgtataac accgggcgtc tggctggcac aggatatctt tctattctgc cggttgacca
    11701 gggcgttgag cactctgccg gagcttcatt tgctgctaac ccgctctact ttgacccgaa
    11761 aaacattgtt gaactggcga tcgaagcggg ctgtaactgt gtggcatcaa cttacggcgt
    11821 gttggtgtcg gtatcgcggc gctatgcgca tcgcattcca tttctcgtca aacttaatca
    11881 caacgagacg ctaagttacc cgaataccta cgatcaaacg ctgtatgcca gcgtggagca
    11941 ggccttcaac atgggcgcgg tggcggttgg tgcgactatc tattttggct cggaagagtc
    12001 acgtcgccag attgaagaaa tttctgcggc ctttgaacgc gcgcacgagc tgggcatggt
    12061 gacagtgctg tgggcctatt tgcgaaactc cgcctttaag aaagatggcg ttgattacca
    12121 tgtttccgcc gacctgactg gtcaggcgaa ccatctggcg gcaaccatag gtgcagatat
    12181 cgtcaaacaa aaaatggcgg aaaataacgg cggctataaa gcgattaatt acggttatac
    12241 cgacgatcgc gtgtacagca agttaaccag cgaaaacccg attgatctgg tgcgttatca
    12301 gttagctaac tgctatatgg gccgggctgg gttgataaac tccggcggtg ctgcaggcgg
    12361 tgaaactgac ctcagcgatg cagtgcgtac tgcggttatc aacaaacgcg caggcggaat
    12421 ggggctgatt cttggacgta aggcgttcaa gaaatcgatg gctgacggcg tgaaactgat
    12481 taatgccgtg caggacgttt atctcgatag caaaattact attgcctgat acgcctcata
    12541 cgaacagcac caccccgtag gacggataag gcgtttacac tgcatccggc aacctatgcc
    12601 tgatgcgacg cggaagcgtc ttatcatgcc tacatagcac tgccacgtat gtttacaccg
    12661 catccggcat aaaaacacgc gcactttgct acggcttccc tatcgggagg ccgttttttt
    12721 gcctttcact cctcgaacaa ttttcatatt gtcgtttttg tgatcgttat ctcgacattt
    12781 aaaagcaaat aatttcatta tattttgaaa tcgaaagcaa acgacaagat atgaaaatgt
    12841 acgtggtatc gacaaagcag atgctgaaca acgcacagcg cggcggttat gcggttccgg
    12901 cattcaatat tcacaatctc gaaacgatgc aagtggtggt ggaaaccgct gccaacctgc
    12961 atgcgccggt catcatcgcc ggaacgcctg gcacatttac tcatgctggt acagaaaatc
    13021 tgttggcgct ggtcagcgcg atggcgaagc aatatcacca tccactggca attcatctcg
    13081 accatcacac gaaatttgac gatatcgctc agaaggttcg ttctggcgtg cgctcagtca
    13141 tgattgacgc ctcgcatttg ccttttgcgc aaaatatttc acgggtcaaa gaggtggtgg
    13201 atttttgcca tcgctttgat gtcagcgtcg aggcggagct ggggcaactt ggcggccagg
    13261 aagatgatgt gcaagtcaat gaagccgatg cgttgtacac caaccccgca caggcgcgtg
    13321 aatttgccga gggattgatt ccctggcggt cgccatcggc acggctcacg gtatgtatgc
    13381 cagcgcaccg gtgcttgatt tttctcgact ggagaacatt cgccagtggg tgaacttacc
    13441 gctggtgctg catggcgcgt caggattatc gactaaggat attcagcaaa ccatcaaact
    13501 ggggatatgc aaaatcaacg ttgcaacgga gctgaaaaat gccttctcgc agtcgttaaa
    13561 aaattacctg accgagcacc ccgaagcgac cgatccccgg gattatttgc agtcggctaa
    13621 atccgcaatg cgcgatgtgg tgagcaaagt gattgccgat tgtggctgcg agggcagggc
    13681 ataacgcacc tgccatttaa caaggaaaaa acatgaaaac gttaattgcc cggcataaag
    13741 ctggtgaaca tatcggcata tgttcagtct gttctgccca tccgttggtt atcgaagcgg
    13801 cgctggcatt tgatcgcaac agcacgcgca aagtgctgat tgaagcaacg tcaaactagg
    13861 tcaatcaatt tggcggttat accggaatga caccggcaga ctttcgcgaa tttgttttta
    13921 cgattgccga taaagttggg tttgcacgcg aacgcattat tctcggcggc gatcatctgg
    13981 ggccaaactg ctggcagcaa gaaaatgcgg atgcggcgat ggaaaaatcc gtcgagctgg
    14041 taaaagcata tgttcgtgct ggcttcagta aaattcatct tgatgcgtca atgtcctgcg
    14101 cgggggatcc cataccgtta gcaccagaaa cggttgcgga acgagctgct gtgctttgct
    14161 ttgctgcgga aagtgtggcg acagattgcc agcgtgagca actgagctat gtcattggca
    14221 ctgaagttcc ggttccgggc ggtgaggcca gcgccattca gtcagtacac atcacccatg
    14281 ttgaagatgc cgccaatact ttacgtacgc atcaaaaggc ctttattgcc cgtgggctga
    14341 cagaggcgtt aacacgtgtg attgccatcg tggtgcagcc gggtgtggaa tttgatcaca
    14401 gcaatattat ccattatcag ccgcaggaag cgcaggcgct ggcgcaatgg atagaaaaca
    14461 cccgaatggt ttatgaagca cattctaccg attaccagac ccggacggct tattgggaat
    14521 tagtccgcga tcactttgca atattgaaag tcggtcccgc attaaccttt gctttacgcg
    14581 aggcgatatt tgcactggca caaattgagc aggaacttat cgcccccgaa aatcgcagcg
    14641 gttgcctggc ggtaattgaa gaagtgatgc tcgacgaacc gcaatactgg aaaaaatatt
    14701 atcgcacggg ttttaacgat tcattactgg atattcgtta cagcctgtcg gatcgtattc
    14761 gttattactg gccgcatagt cggattaaaa atagcgttga aacgatgatg gtgaatctgc
    14821 aaggcgtgga catcccactg ggcatgatta gtcagtatct tcccaaacaa tttgaacgca
    14881 ttcagtccgg ggaattatca gcaatgccgc atcagcttat tatggataaa atttatgatg
    14941 ttttgcgcgc ctatcgctac ggctgtgcgg aataaggacg gtatatgact aacctgtttg
    15001 ttcgtagcgg aatttctttt gtcgatcgta gcgaagtttt aacctatatc ggtaatgaga
    15061 tgctcgcgaa aggtgtggtt catgatacct ggccacaggc attaattgcc agagaagcag
    15121 aattccctac cgggataatg cttgagcagc acgccattgc aataccgcat tgtgaggcga
    15181 ttcatgctaa gtcgtcagcc atttatctgt taaggccaac aaataaagtt cattttcagc
    15241 aagcggatga tgataacgac gtggcggtat cgttggttat tgcgttgatt gtggaaaatc
    15301 cgcagcagca attgaaactt ttacgctgtt tatttggcaa gttacaacag cccgatatcg
    15361 tcgagacact aattactctt cctgaaactc agttaaagga atacttcaca aagtatgttt
    15421 tagattcaga cgaataaatc cctctgtaac aataaaaagg actatttatg aaacgcaaga
    15481 ttattgtcgc ttgcggaggc gcggttgcga cctctacgat ggcggcggaa gaaattaaag
    15541 agttgtgtca gagtcataat attcctgttg aattaatcca gtgtcgggtt aatgaaatag
    15601 aaacctatat ggatggtgtg catttgatat gtaccactgc cagggtggat cgtagttttg
    15661 gcgatattcc gttagttcac ggcatgcctt ttgtttctgg tgtcggtatc gaagcattac
    15721 aaaataaaat tctgactatc ttacaggggt gacctatgtt ttcagaagtc atgcgttata
    15781 ttctcgacct cggccctaca gtgatgctgc cggttgtcat cattattttt tctaaaatat
    15841 taggcatgaa ggcaggcgat tgctttaaag cgggtctgca tatcgggatt ggctttgttg
    15901 gcattggcct tgtgattggc ttaatgctgg attccattgg tccggcggcg aaagcgatgg
    15961 cggaaaattt cgacctgaat ctgcatgtgg tcgatgtcgg ctggccgggc tcttcaccaa
    16021 tgacctgggc gtcgcaaatt gcgctggtgg cgattccgat tgcgatattg gttaacgtgg
    16081 cgatgttact gacccgtatg acgcgggtgg taaatgttga tatctggaat atctggcata
    16141 tgaccttcac cggcgcgttg ctgcacctgg caaccggttc atggatgata gggatggcgg
    16201 gtgtggtaat tcacgcggcg tttgtttata agctaggcga ctggtttgcc cgcgataccc
    16261 gaaatttctt tgagctggaa ggtattgcta ttccgcacgg tacgtcggcg tatatggggc
    16321 cgattgcggt gctggtcgat gctatcatcg agaaaatccc aggcgttaac cgaattaaat
    16381 ttagcgccga cgatattcag cgcaaatttg gcccttttgg cgaacctgtc accgtgggtt
    16441 ttgtgatggg gctgattatc ggcatcctcg cgggttacga tgtcaaaggt gtattgcagc
    16501 tggcggtaaa aacggcggca gttatgctgc tgatgccacg gatgattaaa cccatcatgg
    16561 atggtttaac gcccatcgct aagcaggccc gtagtcgttt acaggcgaag ttcggcggtc
    16621 aggagttcct gattggcctg gatcccgcat tactgctggg gcatacggcg gtggtatcgg
    16681 caagcctgat ttttattccg ctcaccattt taattgctgt ttgtgttccg ggtaatcagg
    16741 tactgccgtt tggcgatctt gccacaatcg gcttcttcgt ggcgatggcg gtcgcggtgc
    16801 atcgtggaaa tctgttccgc accttaatct cgggtgtcat cattatgagc atcaccctgt
    16861 ggatcgcgac gcaaactatt ggtttgcaca cccaactggc ggctaatgct ggggcgttaa
    16921 aagccggggg tatggtggct tcaatggatc agggcggttc tcccattacc tggttactga
    16981 ttcaggtttt ctccccgcaa aatattcccg gtttcattat tatcggcgca atttatctga
    17041 ccggtatttt catgacctgg cgtagagcgc gtggttttac taaacaagag aaagccgttc
    17101 tcgcagaata atttttacct gagggggagg ttatccccct taacaatcag gagtttttat
    17161 gaaatcagga gtttttatga aatcagtagt gaatgatact gatggtatcg tgcgcgttgc
    17221 aaaaagcgtc attcctgaaa ttaaacatca ggatgaggtg cgggtaaaaa ttgccagctc
    17281 gggcttatgt ggttccgatt tacccaggat atttaaaaat ggtgcacatt attatccaat
    17341 aacgttaggc catgaattta gcggctatat tgatgcggtg ggatccggtg ttgatgattt
    17401 acaccctggc gatgcggttg cctgtgtgcc gttattaccc tgttttactt gtccagagtg
    17461 tttgaaaggg ttttattccc agtgcgcaaa atatgatttt attggctcgc ggcgtgatgg
    17521 tggatttgct gaatatattg tcgttaagcg aaaaaatgtc tttgctctac ccacggatat
    17581 gcctattgag gatggggctt ttattgagcc gattaccgtt ggcctgcatg cttttcattt
    17641 agcgcaaggt tgtgagaata aaaacgttat tattattggt gccggaacca ttggcctgct
    17701 ggctattcag tgcgctgtcg cgctgggagc aaagagtgtg acggcgatcg acattagctc
    17761 agaaaaactg gcactggcaa aatctttcgg tgcgatgcaa acatttaaca gtagcgaaat
    17821 gagcgcgccg caaatgcaga gcgttttacg cgaactgcgc tttaatcagc ttatcctcga
    17881 gacggctggc gtaccgcaaa ctgtcgaact ggcggtagag attgccggtc ctcatgccca
    17941 actggcgctg gtgggcacgt tgcatcagga tctgcattta acatcggcaa cgtttggcaa
    18001 aatattgcgt aaagagctga cggttatcgg cagttggatg aactattcca gcccttggcc
    18061 ggggcaggag tgggaaacgg cgagccggtt gctgacagaa cgtaagttaa gcctggatcc
    18121 attaatcgct caccgtggaa gctttgaaag cttcacccag gcggtgcgtg acatcgctcg
    18181 taatgccatg ccgggcaaag tgttgctcat tccatgaaac cgcgggccag cgtgatgctg
    18241 gcccggtatt gtgcaaaaca gatcattcac caatggtccc ccttcgttta cactagccac
    18301 aattgaatat gggtaaatga cgatgaattc attcgagcga aggaataaga tcatccaatt
    18361 agtgaatgaa cagggaaccg tgcttgttca ggatctggcg ggagtatttg ctgcctcgga
    18421 agcgacaatc cgtgccgatt tgcgctttct cgaacaaaaa ggcgtggtta cgcgctttca
    18481 tggcggtgcg gcgaaaataa tgtccggtaa tagtgaaacc gagacccagg aagtcgggtt
    18541 taaagagcga tttcagctcg ccagcgcgcc aaaaaacaga atagcgcagg cggcagtcaa
    18601 aatgatccat gaagggatga ccgttattct cgacagcgga agtaccacaa tgcttatcgc
    18661 cgaaggatta atgaccgcta aaaatatcac ggtgattacc aacagtctcc cggcagcgtt
    18721 tgccctttcc gaaaataaag acattactct ggtcgtctgt ggtggcacgg tccgccataa
    18781 aacgcgctcg atgcatggtt ccattgccga gcgttcattg caggatatta atgccgattt
    18841 aatgtttgtc ggtgctgatg gtattgatgc ggttaatggt attacgacct ttaatgaagg
    18901 ttattcgatt agcggggcga tggtcaccgc cgcaaataaa gtgattgccg ttctcgattc
    18961 atcgaaattt aatcgtcgcg gttttaatca ggtactgcca atcgaaaaaa ttgacattat
    19021 tgttaccgat gatgctgtgt cagaggtgga taaattggca ttgcagaaga cacgggtaaa
    19081 attaattacg gtgtagtatt gttctgtatg ccggataagg tgtttacacc gcatccggca
    19141 ttacggattc tccctggttc aagagagaat agtttgatta acgcaataat ggacaatctg
    19201 gtggtaatcg acaacgcaac gccgccggaa gtatttcaat atggaagttt tgcccactca
    19261 acggttcgcc atcaagatta aaagtgattt cgtgtggggc ttgtatatca aaccacgacg
    19321 aagcgccttc gataatattc gggttatctt cgtcagattt taaggttgat acgagagccg
    19381 gaagaatttc atcgccggta aaaatgcgca gttgcagcaa gccatcgtta attaacgcgt
    19441 tcgggcacaa ttgttgaccg ccaccggcct gacgcccgtt accaatacca atgaccaggg
    19501 cgtcaccttg ccagtgaaag ttttcaccgc ggatttcaca acggtccggt tgcagagtat
    19561 ccatgcgcat taagccatga atgatgtaag agacgccacc cagcgcggct tttaattttt
    19621 ccggcgtttc tgtggtaata cgcgtcccaa atccgcctgt cggcatatta ataaaacagg
    19681 tttgtttgtt gacctgcgcc atatctatcg caatggcatt accggcaatt gccagtttca
    19741 gcgccttatc cagtgcctca ggaatcccta cactggtggc aaaatcattg gcggttccta
    19801 atggcaaaat tcccagcgcg ggtatgtcat ccccctcaca ctgaatcaac gccgtagaaa
    19861 cttcattaat ggtgccatcg ccaccaccgg caatcaccgt tgcgacgccc aacttccggg
    19921 cctcctctac aaatcgtgcg gcatcacctt tctcccaggt gacccgcaca tggatcgtca
    19981 ttccttcctc acgcaacagc ataattgctt cgcgcaaggg tagattgtca gtacttttgc
    20041 cattaagaat cagtaagctg gcgggaaatt ctgccatgat cgtatgtgcc tttatgattg
    20101 gtctgtagat agtgtagagc agaaaacaaa aggtggagtc agaacaggat gaaagtcgga
    20161 ggataaaaag tcagttcaag tatggaggat tcagtggtta ccaccaggag cttgcaatgg
    20221 ggacagcgaa tactaacggc tgccaccgat aaatttcaaa aaagagcata tacctaatat
    20281 tcaactaaac agtggcatct tcaatataat atattaaagc ccccatggag ttaccctgaa
    20341 gggcctcaat gtccgtaatt cctacttatg taggaaatgt tgtacagaac atttattata
    20401 atcctattca aatataataa tcatgccatt attatattta aacactagag agtgtcgttg
    20461 gtatttaatg ggggaaggtg agatgaaaaa gatagctgct atatcattaa ttagtatttt
    20521 tcttatatct ggttgtgctg tgcataatga tgaaacaagt atcggtaaat ttggtcttgc
    20581 atataaaagt aatattcagc gtaaactcga taaccaatac tacaccgaag ccgaagcttc
    20641 tttagccagg ggcagaatat ctggtgcaga aaatatagta aaaaatgatg cagcccattt
    20701 ctgtgttact cagggcaaaa aaatgcagat agttgacctg aagacagaag gtgcaggatt
    20761 acatggcgtc gctcgtctga cattcaaatg tggagagtga gaatattttt tggtaagcgt
    20821 caaacatgcg cgttctggct gtgcgtagcc ggaacctggg cgagcacgat accgttacgt
    20881 gaaaggcatt gtgctatgaa gggggattct atcgatgtgg tcaatggaag acggttatca
    20941 gggatcaatc cttctttaca gcttatgtac aaaaaataag cccgtgtaag ggagattaca
    21001 caggctaagg aggtggttcc tggtacagct agcattttat gggttatgtt tttcagcgaa
    21061 acggatgata accttaataa atgcagctgt atgtgatcgg tttctaagaa ttttccatcc
    21121 gggaaaaata atcgaaatta atcacttacc gtgggggtta cgcgtggttt ccccggagaa
    21181 attacgcatt agcagcgcgt aatttagctc aagatcctgc gggaccggga gccacacagt
    21241 ataaccatcg cctggtgcga ccggcatagc ttcgcctttg gcgttttcca tgtgctcaag
    21301 ggtaaagtta atgttgcctt gcggcgtcat cagctcaaga ctgtcgccaa cggagaattt
    21361 atttttcacc gctaccgctg cgaggtcccc cttgcgctca ccggtaaact caccaacaaa
    21421 ctgctggcgg tcagaaactg aataaccgta ttcgtagttc tgataatcgt cgtgagtatg
    21481 acgacgcagg aaaccttcgg tatagccacg atgcgccaga ccttccagag tttccagcag
    21541 gctggtatcg aacggttttc ccgcagcggc gtcatcgata gctttgcggt aaacctgtgc
    21601 ggtgcgtgcg caatagtaga aagatttggt acgaccttcg attttcagcg aatgcacgcc
    21661 cattttggtc aggcgttcta catgggcgat ggcgcgcaga tctttcgagt tcatgatgta
    21721 agtgccgtgc tcatcttcaa acgcggtcat atactcgccc ggacgctggg cttcttcgat
    21781 cataaacact ttgtcggttg gcgcgccgat acccagcgtc ggctcaacat tttgcaccgg
    21841 aatcggctcg tacttgtgta cgatgttgcc aacgtcatct tctttccctt cctggacgtt
    21901 gtactcccag cggcaggcgt tggtgcaggt gccctggttc gggtcgcgct tgttgatata
    21961 gccagagagc aggcagcgac cggagtaggc catgcacagt gcgccgtgaa cgaagatctc
    22021 gatctccata tccggcacct gattatggat ctcttcaatc tcttccagcg acagctcgcg
    22081 agagaggatc acgcgggtca gtcccatttg ctgccagaat ttcaccgtcg cccagttcac
    22141 ggcgttagcc tgcaccgaga ggtggatcgg catttcaggg aagtgctcac gcaccagcat
    22201 aatcagccct ggatcggaca taatcagcgc atccggcccc atttccacca ccggtttcag
    22261 gtcacggata aaggttttca gcttggcgtt gtgtggtgca atgttgacca cgacataaaa
    22321 ctttttcccc agcgcgtggg cttcattgat gccgagctga agattttcgt ggttgaattc
    22381 gttgttgcgc acacgtaggg agtaacgcgg ctggcccgca taaacagcat ctgcgccata
    22441 agcgaaagcg taacgcatat ttttcagcgt tcccgccggg gaaaggagtt ccggtttaaa
    22501 cataattctc tcgttctgat gacaggtcag atccgcttca cctgatgaag cggttagggg
    22561 aggcccccac tttaagggcg ggcattgtag cgctgtaggg gggatctggt aaagcgccgg
    22621 acgccaacaa cccacgcccg gcatcgtaat tacgtattgt ctttcaccgt ctggcttgtg
    22681 ccattggctt taacggaggc aattccggtt tcacgagatt gcgcggtggc gtacatctgg
    22741 ctggagccga taatttgatg attagcggct ttcagattga aatagaattt gccgttgctt
    22801 gccgtttttt tctcatagcg ctcttcttgc gggctgttgc tacgcaccga cgcgatgccc
    22861 ttttccgcag aggcttttga ggtatacagc tcgctggtga ggatagtctc accattgccc
    22921 gcttttagca caaaccggaa ctgattatca ctgctcttac tgagttcaaa ccaaccagcc
    22981 ataatgactc cttgattgaa attgcttatc gaaaacagca tctaaaggtg ctgtttactg
    23041 attaaagtat gagcgatttt ctgatgcctg ccagggcgga gagataatgt cagtttgctg
    23101 accatgcgtg gaataatgtt gccggatcgg gtctgaacgc catctccggc taacaaagta
    23161 atgtcgctaa aactaaacga tgcggcaggc gtcggcttcc cagcggtaac caacgccgta
    23221 aacggcgcgg ataaatgact gttcggcgtc gagagattcc agcttgcggc gcagattttt
    23281 aatgtggctg tcaatggtgc ggtcggttac tacgcggtag tcgtcataaa gatgattgag
    23341 caattgctcg cgggagaaca cttttcctgg ttcgtgagag agcgttttaa gcagacgaaa
    23401 ttccgcaggc gtcaggtcaa gcattttacc gcgccatgaa gcctgaaaac gaccttcgtc
    23461 gataatcaac gggctttcgg catcctgttg ctgaagttcg cgttgcggtt tacaacggcg
    23521 caaaatggtt ttgacacgcg ctaccacttc ccgtgggctg tacggcttgc agatgtagtc
    23581 atcggcgcca atctccagcc ccagcaggcg gtcgatctct tcgatttttg ccgtcaccat
    23641 cacgatcgga acatcggaaa aacgacgaat ttcccggcat aatgtcaggc catcggtgcc
    23701 agggagcatc agatccaaca ggatcagatc cggtggtgtc tggcgcacat acgccagtac
    23761 ctgatcgccg tggctgataa gcgtcggcgc atagctcaca gcacgcagat aatcaatgag
    23821 caactgcccc agcttcggtt catcttccac gatcaaaata cgcggtgtgt tttcgtcgat
    23881 tggtaactcg gtcatacttc tctctgtaaa tcccgttcca gcggtaactc tactgtaatg
    23941 cttaccccgc caaaaggcga atgggcggca ataatgcggc cattatgggc ttcaacaatg
    24001 ttcaggcaaa tcgccagccc cagcccggaa ccgccgctgg cgcggttgcg ggaaccttcg
    24061 gtgcgataaa aacgttcaaa caatttttgt agctgatcgt cactgacacc tggcgcactg
    24121 tcggcaaagg tcaggcgcac cgttttgtcg tgctgctcgg cagagatttt caggctgccg
    24181 ccgctgtcag tgtagcgcag gctgttttcc agtaagttat tgaataactg cattaaacgg
    24241 tcgcgatcgc caaatacggt gatactgtct ggcagggaaa attgcagttt caggccacgg
    24301 ctggcgaatc gttcgcgaaa tgcgccgccc gccacttcca gcagtgggat caaatctacc
    24361 ggtgcttttt gataggcgag agcgccttca tcagacatcg acaactgatg aagatcgtca
    24421 accagtttgg tcagtgtacc gacctccgcc tgtaaagacg ccaccgtctc cggcgtgaat
    24481 ttgcgcacgc catcctgaat ggcttccagt tcaccgcgca gcaccgctaa tggcgtacgc
    24541 agttcgtgag aaatatcggc cataaaatcg cgccgcattt gctggttttt ctccagcgtg
    24601 ctggcgagct ggttgaagtc ttgcgccagt ttgcccagtt catcttcact ggtgggcgtt
    24661 acgcgggtag tgaaatcgcc cgccgccagt ttgtgcgtgc catcgacaag tcgttttacc
    24721 ggtgccagca aaccgcgcgc cagcagaaag gtggcgagcg ccgcgagtaa cgttgccagg
    24781 gcgacgatca gccagctggt ttgccgctgt tgtttatcga aattgatatc agtattgcgg
    24841 gtcagccgct caacagggga ggcaatcacc gcgcccactt ccgcaccgtt gaccagaatg
    24901 ggtcgccgtg taccgtcagg tggaatcggc gctcgcggac caaccagtac tttgttgttt
    24961 tgatcgacca cccagaactg ggtacgccag ccgtgcggcg gcatacccgg gccgggttta
    25021 tcttccgaat tatcgtgttc aaatgaacgc aggatctgaa agacaaagcg atcgttgttg
    25081 cgcaggaagc gccagttacc atgctgcgca tactgctcgc caagcgcatc acttaacagc
    25141 tgtaatcgct gttcattgcc gtgtttgatg taatcaataa agccacgctc aaaactgata
    25201 cgcaccgccc agtgcatact gatcagcaag acaatgcagg tggcgaaaat cgccagaaac
    25261 agtttgccgg taataccggg tcgccagaac ttcattgcgc gctccttttt cgccgcgaaa
    25321 tagctacatt ttgatgcgta tcgttcggca ctctggcaaa gatgaacgcc ggaagggcga
    25381 tgataaacgc catgctaagc caggtgtaca taaagacggt ttgtgtggtg ccgctgtcga
    25441 cgctgacatg ctgcgaacca aaaagcccca gcaacagccc ggcgatggtg acgccgatac
    25501 tcatcgacaa ttgcataatc atcgacagca ggctgtttcc gctgctcgcc agattgtccg
    25561 ggagatcttt cagcgtcagg gtgttcatgg aggagaaacg ggtcgagttg accatccctt
    25621 gtaaaaacag gacgaacggc aaaacgtagt accagcccaa cagagcggta gtcataaaca
    25681 acagggtgac cagcgacaga cccagcgtgg tcgctaccag tacccgacga taaccaaagc
    25741 gattcaccac ctgtaccaca attcgcttca ttcccatgct gccaagcacc atcgggatca
    25801 tcatcagtcc ggcatgaaac ggcgagaaac cgaggccaat ttgcaggaaa accggtgtca
    25861 taaagggcaa catgccactg ccaatacgtc cggcaaagct ccccgccagg cccagcgaaa
    25921 aggtacgagt gcggaacagt ttcagactga acagggcgcg gttgttattt ctggcgtgca
    25981 gcagataaag caccagtgcc accacgccaa ctgcgaccag gccagcaatc gccagcggcg
    26041 ataaacctgt acctttactg ccgtccagcg ccagggttaa taccgccatg ccaaccgcca
    26101 gcaataaaaa gccggaaaga tcaaagcgcc gcgtctgcat ggtgtagttc ggcattaaca
    26161 tcaatgtggc gatcgcaccg ataatcccca ccggaatgtt gatcaaaaag atccagtgcc
    26221 acgacgcgta ttctaccagc agaccgccga gcgccggacc gagcagcgga ccgacctgac
    26281 cgggtaacgt gacaaaggtc atcgccgcca tatattgctc gcgcggtacg attttcatca
    26341 ccgtcaacct gccgaccggc accatcatcg caccgccaac gccctgtaac gcgcgtgcca
    26401 gcaacagttc gttcagcgtg ccggaaagcg cgcaaaacag tgaaccgaga gtaaacagca
    26461 cgatggcggt aaagaaaata ttgcgcacgc cgactttgtc cgccagccag ccgctggcgg
    26521 gcagcatcac cgccacggtc agcacataag agacaatgac catgtgcata tgcaacggac
    26581 tttccccgag gctttgcgcc attgagggaa gggcggtgtt tacgatggtg gtgtccagcg
    26641 actgcataaa gaagccgaaa gccacaatcc ataattgcca gcgggtgctg tcgggaagat
    26701 ctgtcattta ctcggttacc gtttgtttag gtttacgcga aaaacgcagc cgcagacggt
    26761 cgaaaaagag atacaccacc ggcgtggtat acagcgtcag gagctggctc atcaccagac
    26821 cgccgacaat ggtgatcccc aggggttgcc gcagctccga gccgtcaccg cccgacaata
    26881 ccagcggcag cgcgccaaac agcgccgcca gggtggtcat cataatcggg cgaaaacgca
    26941 gcagacaggc ctggaaaata gcttcctgcg gcgtcaggtt accgtgccgt tgggcttcaa
    27001 gcgcaaaatc gaccatcata atggcgtttt tcttcacgat gccgattaat agcatgatcc
    27061 ctattagggc gattaggctg aacggggcat tgaacagctc cagcgccaac agcgctccaa
    27121 cgcccgccga gggcagggtg gagagaatcg tcagcggatg tacgtaactc tcgtaaagga
    27181 tacccagcac gatatacacc gtggcgatgg cggcgataat caggatcacc tgcgagttca
    27241 tcgtctcctg gaaaacctgc tccgtgccgg caaaactgcc gcgcaccgtc gaaggcacac
    27301 caagctgggt cattgcgcga tcgatcgccg cactggcgtc cgagagcgat tttccggtcg
    27361 gcaggttgaa cgagatggtc gaggccgccg ataatccctg atgattcacc gatagtgggg
    27421 cattcgccgg ttgccattta gcgaaatacg acagcgggat cgctttgcct tcgttattga
    27481 taacgaacat tttttccagc gcactgatgt cctgggtata gcgcggatcc acttccatca
    27541 ccactttata ctggttcatc ggctggtaaa tggtcgagat ttgccgctga ccgaaggcgt
    27601 tatttaacag actgttggcg gcctgtacgt cgattcccag ccgtgccatg gtgtcgcggt
    27661 cgtaaaccag attcatctcc gcgccgttat cctgctgatc ggagttcacg tccgccagtt
    27721 ccggcaacgt cgccagtttt ttgcggattt tcggctccca ttcgcgcagt gccgccaggt
    27781 cgtcggataa caacgtgtac tggtagctgg cgttcgactg acgcccacca acgcgaatat
    27841 cttgtaccgc catcaggaac agattcgccc ccggttcttt cgccagcttc acgcgcagac
    27901 ggtcgataat ttgctgcgcc gtttcgctgc gttcgtcgcg tggcttgagg gtgataaaca
    27961 tcatcccact gttaactcgt gaaccgccgg taaagccggt gacattatcc actgccggat
    28021 cgtcacggat aattttcatg aaatcctgca atttgccgcg catcgcctga aacgaaatac
    28081 tctgatccgc ctgtatccca cccatcagta cgccggtgtc ctgctccggg aagaaggttt
    28141 tcgggatcga gatatacagc cagatattca gcgcaatggt gccaagcagc accacgccca
    28201 ccagacgggt atgattgagc acccatttca gcgacttgcc gtagccttgt tgcagggcta
    28261 ccaacatgcg gccaaaacca cgcagtcgct tttgctcacg cggcttgctg gcttttagca
    28321 tccagccgca catcattggc gttagcgtca gggaaaccag caacgaaata ccaatggcga
    28381 cagaaagcgt cacggcaaac tcgcgtaaca gtcggcccgg caatccgccc atcaacagca
    28441 gcggcaggaa caccgccacc agtgacagac tcatcgacag caccgtaaag ccgacttcgc
    28501 gagtaccttg cagcgcggcc tgtagtggtt tcatccccgc ttccagatga cgggcaatgt
    28561 tttccagcac cacaatggcg tcatccacca cgaaaccagt agcaatggtg agcgccatca
    28621 gcgaaagatt attgagactg aatccgcaca ggtacatcgc cgcaaacgta ccaatcagcg
    28681 aaaccggcac cgcaacggcg ggaataatag tggcgcgacc cgagcgcagg aataaaaaga
    28741 ccaccagaat caccagcgcc accgagataa tcagcgtttg ctcgacttct tccagcgagg
    28801 cgcgaatggt gggggagcga tcctgggcaa tttgcagatc aatcgccgcc ggaatggtct
    28861 cctgcaactc tggtaatttt gcccggatgc tgtctaccgt ctggataata ttggcttccg
    28921 gcagtttgcg gatcatcagt aaaatagccg gtttggcgtt ggtcatcccg gcgttgcgca
    28981 catcctgcac tgagtcggtc accgtcgcca catcgcccag acgaaccgcg ccgccgttgt
    29041 tgtagtgaat aatcagcggc tgatattcag cggcggtttt cagttcatcg ttggtctgga
    29101 tctgccagcg gtgagtgcca tcttccagcg ccccttgcgg tttacgcacg ttggcattgc
    29161 tgatggcagt gcgtacgtcg tccagcgaca cgccctgatt aaacagcgcc tgcgggttca
    29221 gcccgacgcg cacggcgggc agcgagctgc caccgacatc gacatcaccg acaccgtcga
    29281 tttgcgaaat cgtcggagcc agctgcgtcg aggcgaaatc gtacagttca ccctgcgaat
    29341 aagtatcgga tgtcagcgtg aggatcataa ttggcgcatc cgacgggttc gctttgcgat
    29401 aggtcgggcg gctgggcatc ccgctgggca gcaaactttg tgcagcgttg atcgccgcct
    29461 gcacatcacg cgctgcgccg ttgatatccc ggtcaaaatc aaactgcaaa ataatgcgcg
    29521 tgctgccgag cgaactgctg gaggtcattt cactaacccc ggcaatgcgc ccaagtgagc
    29581 gctccagcgg cgtggcaacg gaagacgcca ttgtctccgg cgacgcaccg ggcagcgagg
    29641 cgctgaccat aatcaccgga aaatcgacct gcggcagcgg ggcgaccggc agcatacgga
    29701 aacccagtat gccgcacagg gtaatggcga ccgacagtaa aatcgtcgcc acagggcggt
    29761 aaatgaagag ggcaaaaaac ttcacttacg cctcctcttc atggcgagca aagcgacttt
    29821 tggtccacaa tgccaggcgg tcgaacagca gataaatcac cggcgtggtg aacagcgtca
    29881 gcacctggct gacaatcaga ccgccgacca tgccgatacc taacggacga cgcagttccg
    29941 cgccgacccc ggtactcaac atcagcggca gcgcgccaag cagagccgcc agggtggtca
    30001 tcaggatcgg acgaaaacgc aacagacaag cctggtagat tgcatcgcgc ggcgacatgc
    30061 cttgctcgcg ctcagcagcc agcgcgaagt cgatcatcat gatggcgttc ttcttcacaa
    30121 taccgatcag caaaataatg ccgataatcg caatcacatc cagttcgcta ccggcaatca
    30181 ttaatgccag cagcgcgccg acccctgcgg tgggtagcgt cgagagaatg gtgatcgggt
    30241 gaataaagct ctcgtacaga atgccgagca cgatatacat cgccaccacc gccgcgacaa
    30301 tcagccagac agtgctgccc agcgccgact ggaaggcgag ggtgctgccc tggaattgcg
    30361 tggtgatatc caccggcaga ttcagcgtct tttcggtgtc cataatcgcc tgcaccgcat
    30421 cgcccagcga atagttatcc ggcacgttaa aggagatggt cgttaccggg aactgatcca
    30481 gatggttgat ggaaagcggc gcaaaacgct gctcaatttt ggcaattgag cttaacggca
    30541 ccacgccacc gtcgctgctg gtcaggcgaa tggtatccag cgccgcgagg cctggggtat
    30601 tttcggtatt gtgctccagc accacgcgat actggttagc ctgagtataa atagtggaaa
    30661 tcagtcgctg accaaacgcg ttgtacaggg cgttatcgac gtccgccatg ctgatcccca
    30721 gacggctggc gctgtcgcga tcaacattga catacgccac cagccctttg tcctgccagt
    30781 cgctggagac atcagaaagc tgtggcagtt gctggagttt ttccatcaac tgtggcaccc
    30841 aggtactgag cgcatccagt gacgtggcct gcaaggtaaa ctggtactgg gtgcggctga
    30901 cctgagtatc gatggtcagg tcctgcgttg gttgcaggaa gagatcgacg cccggcactt
    30961 tatctaccgc cgtttgcaga cgggcgatga ctttttgcac ccgatcatca cgttcatcca
    31021 acggtttgag gttgatttgt aaacgtgcac tattcagcga cgggttagtg ccatcaacgc
    31081 caacaaatga ggtcaggctt tgcactgccg gatcctgcaa aatcacgtcc gcgacctggc
    31141 gttgtcgctg ggccatattg gcaaaggagc tggattgcgg tgcctgcaaa gtgccctgaa
    31201 taatgccgtt gtcctgtacc gggaagaaac ctttcggaat gaacacccac agcagcacgc
    31261 taagcagcag cgtgctgagt gccacgctta aggtcagcca cggatgattc agcactttcg
    31321 ccagtccacg accataggcg gcgattatcc tgtcgaacat tttttccgag gcacgggaga
    31381 agcggttctg tttacgcaac gactcctggc tgagcatccg cgcgcacatc atcggtgtca
    31441 gggtcagcga caccaccgct gagatcaaaa tcgctaccgc cagggtaata gcaaattcgc
    31501 ggaacagtcg cccgacgata tcgcccataa acagcagtgg gatcaacacc gcaatcagtg
    31561 agaaggtcag cgagataatg gtaaagccga tttcacctgc gcccttgagc gccgccgcca
    31621 acggtttttc gcctttttcg atatagcggg aaatgttttc gatcaccacg atggcgtcat
    31681 cgaccacgaa tccggtggcg atagttaacg ccatcagtgt caggttattg attgaaaaat
    31741 cgagaaacac cataaccgcg aaagtgccga ttaacgacag cggtacagca acaccgggaa
    31801 tgatggtcgc cggaatattg cgcaaaaaca ggtagataat catgactacc agcgcgatag
    31861 ccatcatcaa ttcaaactga gtatcatcga cggatgcgcg gatattggtg gtgcgatcgg
    31921 aaagcactgt caccttcacc gatttcggca gactctcagt gagctgtggc agcatctgcc
    31981 gaatgctgtc ggcggtggag ataatgttag caccgggctg gcgctgaaca ttcatcacaa
    32041 tggcctgttc tttgttcgcc cacgcgccga gccagctgtt ttctgcacct tgctctacag
    32101 ttgcgacatc gcccagacga attggcgcgc cgttctggta ggcgatgatt agctggcgat
    32161 actcttcggc ggattgcatc tggtcgttcg cggaaagcgt gaccgcacgg gaagggccgt
    32221 cgaggctacc ttttgccgag ttaacgttag cgccggtaat ggcggtgcgc acggtttcgc
    32281 tggtcaggcc gagggcggca atcgcctgag cgttaagttt gacgcgaaca gccggacgct
    32341 gaccgccgga aagcgtcacc aggccgacgc cggaaatctg cgagattttc tgcgcgacgc
    32401 gggtttccac catatcttcc acttgcgtca tcggcatggc ggttgaggtg acggcgagcg
    32461 tcatgatcgg cggatctgcc gggttcactt tgctgtaaac cggcgggtta ggcagatcgc
    32521 tcggcaacaa gttggtcgca gcgttaatcg cggcctgcac ttcctgctcg gcgacattga
    32581 gcggtaatgt tagctggaac tgcaaagtga taactgacgc accgccggaa ctttgcgacg
    32641 acatctgttt caggccagac atctgcccga actggcgttc gagcggcgcg gtaacggcag
    32701 aggtcatgac atccgggctg gcacctgggt agagcgtgac cacctgaatg gtcggatagt
    32761 ccacttccgg cagcgccgaa acgggcaggg cgcgataacc gataatcccg gcgagtaaga
    32821 tcgccaccat cagcagcgtg gtggcgacag gacgcataat aaacaggcgc gacgggccgc
    32881 ctgtgctgct cgggggtaac acctgcatca ggagcgtgct ccttttttcg cgtattcgcg
    32941 gctggtggct ttctcttccg gagtggtggc gctctgggct tccaccactt ccactttcgc
    33001 cccttcggtc aggcgatcaa tgccgtctgt caccacgcga tcgcccgcag aaatacctgc
    33061 acggatcacc actttctgac tgtcctgaat gcccggcgtc accagatgtt tgctgacctt
    33121 gttttcgcta ttcagcaccc agacaaaatg gccttcattg cccatttgca gggcggctgt
    33181 tgggatcact acggcgtttt gttcggtgtc gactaacatg cgcgcgttaa caaactgatt
    33241 gggaaacagc gcatcatcct gattattaaa gcgtgctttc actttaatcg taccggttgt
    33301 ggcatcgatt tggttatcga ggcttaacag cgtgccttca cttaatttct tcgaattggt
    33361 gcgatcccag gcttctacca tcagcggttt tccggctttt tgcgcctgca ctacagtagc
    33421 gatatcgctt tccggcaggg taaagactaa atcgatagga tgcgtctggg tgatcaccac
    33481 aattccggtg gtatcaccac tggagatttg gttaccaaca tcaacttgct tgagaccaac
    33541 gcgaccatcg actggtgcgg tgatgcggct ccagtcgagt tgcaactgcg cgctggcgac
    33601 gcttgcttca tcagccttaa tggtgccttc ggtttcactg accagcgcct gttgggcatc
    33661 cagctcctga cgggagacga gattggtttt tgccagttgc tgataacgcg ccagatcacg
    33721 gcgggcgtta gcaagcgtgg ctttatcttt tgccagttgg ccctgggttt gtgctaatgc
    33781 aactttgaac tggctggggt caatttctgc cagtaaatcg cctgctttga cctgctggcc
    33841 ttcctggaaa tgtaacgcca tcagttgacc gtccacgcgg ctgcgcaccg taacagtatt
    33901 agcggcggta atggtgccaa gcccggtgag gtaacgcgga actgcctgtt ctacggcggt
    33961 cgccgcctga accggggcta atgggccgga acgcatacca cggcgaccac ccgctggcga
    34021 ttgctgcgct tgtttcgtcg cccctggggc tgcactccgg gagtcattgc ggccttgcca
    34081 gaaccagaat gcggcgatgg cggcgataac caccacgatt acgattaccc aacgggattt
    34141 ataactgcct ttcatcgtta agagtttctc ttcctgaaac gtttcgcgga atgatactag
    34201 tgtagccagc caataaggga gaaaaatgga ggaattatga aacactatcg ggaatcgtct
    34261 gaatggcggg cacatttgcg agcacgcatc cagtaataac acaggaaact attttatcta
    34321 cgcgttagcg atagactgca tgcatggcga aaggaggtaa gccgccgatt tcagcgggac
    34381 gctgaaacgg gaaagcccct cccgaggaag gggccataaa taaggaaagg gtcatgatga
    34441 agctactcat catcgtggtg ctcttagtca ttagctggcc agcttactaa gactaccagg
    34501 gcgggggaaa ccccgctcta ccctcactcc tgaaagtatg ccttcacgat aagattgtca
    34561 atctgcaggc tttgcagtct gcgatcctgc cagcaaatat tctttgcgag acgttacgca
    34621 ataatcacac tggaaactat tttatccgca agtcagcgat agactgcatt cagggcgaaa
    34681 ggaggtaagc cgacgatttc agcgggacgc tgaaacggga aagcccctcc cgaggaaggg
    34741 gccttaaata aggaaagggt catgatgaag ctactcatca tcgtggtgct cttagtcata
    34801 agcttccccg cttactaaga ctaccagggc gggggaaacc ccgctctacc ctcactcctg
    34861 aaattatgcc ttcacgataa gattgtcaat ccgtaggctt tgtagtctgc gatcctgcca
    34921 gcaaatattc tttgcgagtt gttacgcaat aatcacagag gaaagtattt tgttcacgcg
    34981 ttagcgatag actgcattca gggcgaaagg aggtaagccg acgatttcag cgggacgctg
    35041 acacgggaaa gcccctcccg aggaaggggc cttaaataag gaaagggtta tgatgaagca
    35101 cgtcatcata ctggtgatac tcttagtgat tagcttccag gcttactaag aacacc
//
LOCUS       DADLSA010000028        34770 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.028, whole genome shotgun sequence.
ACCESSION   DADLSA010000028 DADLSA010000000
VERSION     DADLSA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.028"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(19..94)
                     /locus_tag="JN691_001365"
     tRNA            complement(19..94)
                     /locus_tag="JN691_001365"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59..61),aa:His,seq:gtg)
     gene            complement(153..229)
                     /locus_tag="JN691_001366"
     tRNA            complement(153..229)
                     /locus_tag="JN691_001366"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Arg,seq:ccg)
     gene            complement(332..1717)
                     /locus_tag="JN691_001367"
     CDS             complement(332..1717)
                     /locus_tag="JN691_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7242848.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1908..2648)
                     /gene="rffM"
                     /locus_tag="JN691_001368"
     CDS             complement(1908..2648)
                     /gene="rffM"
                     /locus_tag="JN691_001368"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAY7242849.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2651..4003)
                     /gene="wzyE"
                     /locus_tag="JN691_001369"
     CDS             complement(2651..4003)
                     /gene="wzyE"
                     /locus_tag="JN691_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAY7242850.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVHFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(4000..5079)
                     /gene="rffT"
                     /locus_tag="JN691_001370"
                     /gene_synonym="wecF"
     CDS             complement(4000..5079)
                     /gene="rffT"
                     /locus_tag="JN691_001370"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAY7242851.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEQ
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5076..6326)
                     /gene="wzxE"
                     /locus_tag="JN691_001371"
     CDS             complement(5076..6326)
                     /gene="wzxE"
                     /locus_tag="JN691_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAY7242852.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6328..7458)
                     /gene="rffA"
                     /locus_tag="JN691_001372"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6328..7458)
                     /gene="rffA"
                     /locus_tag="JN691_001372"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HAY7242853.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7463..8137)
                     /gene="rffC"
                     /locus_tag="JN691_001373"
                     /gene_synonym="wecD"
     CDS             complement(7463..8137)
                     /gene="rffC"
                     /locus_tag="JN691_001373"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HAY7242854.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8115..8996)
                     /gene="rfbA"
                     /locus_tag="JN691_001374"
     CDS             complement(8115..8996)
                     /gene="rfbA"
                     /locus_tag="JN691_001374"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY7242855.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIRKILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQCFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9015..10082)
                     /gene="rffG"
                     /locus_tag="JN691_001375"
     CDS             complement(9015..10082)
                     /gene="rffG"
                     /locus_tag="JN691_001375"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY7242856.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQGERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10082..11344)
                     /gene="wecC"
                     /locus_tag="JN691_001376"
     CDS             complement(10082..11344)
                     /gene="wecC"
                     /locus_tag="JN691_001376"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAY7242857.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11341..12471)
                     /gene="wecB"
                     /locus_tag="JN691_001377"
     CDS             complement(11341..12471)
                     /gene="wecB"
                     /locus_tag="JN691_001377"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAY7242858.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12527..13573)
                     /gene="wzzE"
                     /locus_tag="JN691_001378"
     CDS             complement(12527..13573)
                     /gene="wzzE"
                     /locus_tag="JN691_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAY7242859.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13585..14688)
                     /gene="wecA"
                     /locus_tag="JN691_001379"
     CDS             complement(13585..14688)
                     /gene="wecA"
                     /locus_tag="JN691_001379"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAY7242860.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14928..16187)
                     /gene="rho"
                     /locus_tag="JN691_001380"
     CDS             complement(14928..16187)
                     /gene="rho"
                     /locus_tag="JN691_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAY7242861.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16272..16373)
                     /gene="rhoL"
                     /locus_tag="JN691_001381"
     CDS             complement(16272..16373)
                     /gene="rhoL"
                     /locus_tag="JN691_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAY7242862.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16514..16843)
                     /gene="trxA"
                     /locus_tag="JN691_001382"
     CDS             complement(16514..16843)
                     /gene="trxA"
                     /locus_tag="JN691_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAY7242863.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16974..18239
                     /gene="rhlB"
                     /locus_tag="JN691_001383"
     CDS             16974..18239
                     /gene="rhlB"
                     /locus_tag="JN691_001383"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAY7242864.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18373..19857
                     /gene="gppA"
                     /locus_tag="JN691_001384"
     CDS             18373..19857
                     /gene="gppA"
                     /locus_tag="JN691_001384"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAY7242865.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19904..21925)
                     /gene="rep"
                     /locus_tag="JN691_001385"
     CDS             complement(19904..21925)
                     /gene="rep"
                     /locus_tag="JN691_001385"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAY7242866.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22012..22293
                     /gene="ppiC"
                     /locus_tag="JN691_001386"
     CDS             22012..22293
                     /gene="ppiC"
                     /locus_tag="JN691_001386"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HAY7242867.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22339..23814)
                     /gene="ilvC"
                     /locus_tag="JN691_001387"
     CDS             complement(22339..23814)
                     /gene="ilvC"
                     /locus_tag="JN691_001387"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAY7242868.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGAYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     CKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23958..24848
                     /gene="ilvY"
                     /locus_tag="JN691_001388"
     CDS             23958..24848
                     /gene="ilvY"
                     /locus_tag="JN691_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAY7242869.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24845..26389)
                     /gene="ilvA"
                     /locus_tag="JN691_001389"
     CDS             complement(24845..26389)
                     /gene="ilvA"
                     /locus_tag="JN691_001389"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAY7242870.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26392..28242)
                     /gene="ilvD"
                     /locus_tag="JN691_001390"
     CDS             complement(26392..28242)
                     /gene="ilvD"
                     /locus_tag="JN691_001390"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAY7242871.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(28307..29236)
                     /gene="ilvE"
                     /locus_tag="JN691_001391"
     CDS             complement(28307..29236)
                     /gene="ilvE"
                     /locus_tag="JN691_001391"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAY7242872.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSLSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29256..29519)
                     /gene="ilvM"
                     /locus_tag="JN691_001392"
     CDS             complement(29256..29519)
                     /gene="ilvM"
                     /locus_tag="JN691_001392"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAY7242873.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29516..31162)
                     /locus_tag="JN691_001393"
     CDS             complement(29516..31162)
                     /locus_tag="JN691_001393"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAY7242874.1"
                     /translation="MNGAQWVVHALREQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMVEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31165..31215)
                     /locus_tag="JN691_001394"
     CDS             complement(31165..31215)
                     /locus_tag="JN691_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242875.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31301..31399)
                     /gene="ilvL"
                     /locus_tag="JN691_001395"
     CDS             complement(31301..31399)
                     /gene="ilvL"
                     /locus_tag="JN691_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAY7242876.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31751..33271
                     /locus_tag="JN691_001396"
                     /pseudo
     CDS             31751..33271
                     /locus_tag="JN691_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(33296..33634)
                     /gene="maoP"
                     /locus_tag="JN691_001397"
     CDS             complement(33296..33634)
                     /gene="maoP"
                     /locus_tag="JN691_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAY7242877.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33753..34592
                     /gene="hdfR"
                     /locus_tag="JN691_001398"
     CDS             33753..34592
                     /gene="hdfR"
                     /locus_tag="JN691_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAY7242878.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(34689..34764)
                     /locus_tag="JN691_001399"
     tRNA            complement(34689..34764)
                     /locus_tag="JN691_001399"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34729..34731),aa:Trp,seq:cca)
BASE COUNT         8475 a         9516 c         8782 g         7997 t
ORIGIN      
        1 tgtcacaact tctaataatg gggtggctaa tgggattcga acccacgaca actggaatca
       61 caatccaggg ctctaccaac tgagctatag ccaccactac aaatcttgtt acgcggtatt
      121 actaccaccg cagctcaagc gccgggacta aatggcgcgc ccgacaggat tcgaacctga
      181 gacctctgcc tccggagggc agcgctctat ccagctgagc tacgggcgct tagcgccgtt
      241 gcgggggtgg ataatacgga cttcccaccc ctctgtctag tcccttttta aataaattgc
      301 gcgtttggtt acggtttgtg cgttttgctg cttattcctc cagtttatgc gctttaccgt
      361 ggcgattaag cccaaaaact ttataaatcg ccgtcaccgc cagcataaag atgatgccga
      421 caaacagcga catacgcgta tcttcattaa agtacatgcc gatcaaaacg caaatcagga
      481 atgccattgt tacgtagttg gcccacggga acaggattga gcggaacgga tggctggcaa
      541 tcgccgcttt atgtgcacgc cgaaaacgca gctggcttat caatatcaca aaccatggca
      601 ccatccccgg aagcacgctg gcactgtaga cgtagacaaa cacacgctgc ggattgggaa
      661 tgatgtagtt caggcatgag ccaattagca gaatagcaat agatactgcc acacccgcaa
      721 ccggtacgcc gtgacgggaa acttttgcca ttgccgccgg taactgacgg tttttcgcca
      781 gtgcgtagag catacgtccg caactgtaca tgccgctgtt acagccagag agcgctgccg
      841 tcagcaccac aaagttgata atgcccgccg ctgcggtaat accgattttg gcaaaagtca
      901 gtacgaacgg gctgccgttg ctgccaattt cattccacgg gaagatggtg acgataacga
      961 aaatcgcgcc tacgtagaaa atcaggatcc gccacagcac tttgcctacg gcactgcgca
     1021 gcgtcacctg cggattcttc gcttcaccgg cagtaatgcc aatcagctcc acgccctggt
     1081 aggacgccac cacaatacac agagcggtca ggaacccttt ccagccaccc gcaaagaaac
     1141 cgccatgctc tgtgagattg ctaaaaccaa tcgactgccc gccattgcca aagccaaaga
     1201 aaatcacgcc caggccaatg acaatcatca cgataatcgt ggtgactttg atcatcgcga
     1261 accagaactc gatttcgccg tacaaccgca ccgccgccag attcgccaac gccaccagcg
     1321 ccaccgcgat caatgcgggt atccactgcg ccatctccgg gaaccaaaac tggacataaa
     1381 cgccaatggc ggtgatttct gagatcccca ccgccatcca cataaaccag taagaccagg
     1441 cggtgagata gccaaaaaac gggctcatat aacgatgcgc ataaacggcg aacgaaccgg
     1501 taaccggttc gaggaacaac atttcgccca ttgaacgcat gatgaaaaaa acgaacagcc
     1561 cggcgatgat ataggccaac aatacggatg gcccggccca tttcagggta ctggcggccc
     1621 ccataaacag gccgacgcca atggtgcccc ccagggcgat gagttcgata tgtcgagctt
     1681 ccagcccacg ctgtagctct ggtttgttat ctgccataaa tcctcgtgtt gtgtttgcat
     1741 gctttccggt gttaccggtt atcgttatgg gtacatcgag tgttgcgaat gttttcgtaa
     1801 ttcaggagaa atggcaaata aagcattaaa aatttgaatg ctttgtgtaa taaaaaagca
     1861 gacaggcgac gaagtgacca ctccgtcgct ttacaaagag aggaaaatca gaggttgccg
     1921 gtgtagtgcc agcgtaaata acgcagcaaa cgaagctgac gcttaatgcg gctcggctgc
     1981 gaaagcaggc ggtagagcca ctccagtccc agcgtttgcc agattttcgg tgcgcgtttt
     2041 acgtgaccgg tgaaaacatc gtaagtcccg ccaacgccca tatacagcgc atctggatgc
     2101 accagacggc agtcgcgcat gaagatctcc tgctttggcg atcccatcgc aacggtgacg
     2161 atttgcgctc cgctggcatg aatgcgttca aacagcgcct gacgctgctc gggtttaaaa
     2221 taaccatcct gactgccaac gatattcaca ttccattggt tgcgcagttt agcttcagtt
     2281 tgcgccagca cttcaggttt accgcccaca agaaataccg gcgtcccttc tttgcctgcg
     2341 cgcgccatca gctcttccca gagatcggca ccggcaacgc gggaaacctg cgcctgcggg
     2401 tactttttac gtactgaacg tacaacgctg atgccatccg catatttaaa ttcggcagcg
     2461 ttaattaact ccctgacctc ggcgttatct tcaatggtca gcattttttc ggcattaatg
     2521 gcaaccagcg ttccctgctt aagctgcccg tcagcaaaca gataatcgag ggcatgctgc
     2581 atatcacgcc aaccaatcaa ctgtaagcca cgcagcgtat aggtcggtgc cgtggtgttg
     2641 ttattcattg ttatccttca acctgcgtcc ggagcgatga ttttgtacgt ttatgaatga
     2701 gtccggcgct ttcaaaaagc cagtacaaca gttttgcgat catcagacat gcgccgaaga
     2761 ccacgataaa aaagactacg cgtgagacaa acgaatccag cccttcacgt gccagcacga
     2821 tcatattgaa gatcgcccca aagcagaaac tgtgcaatat cgcagcctta tagcgattag
     2881 tctcgcggtt gcccagctca tacagccagt cgaaccattt gatgatcagt ccaaccacaa
     2941 tcgccccgag tgggatgaac aacgcgccgc ccatcaccac cagtgagcct ataagcgtag
     3001 gcgagatcgc cagtccggag tggttattca gtacttccca ggtaaagtaa ttggctgagt
     3061 tcagcaccat actcgggcgc cccggccaca gccaggaagg gataaagaca tagaaatcgc
     3121 ggacaattgg agccaggccc tggaagtcga tgttgtcgta gttctgcaac agcaacgcca
     3181 gattctccca cggtgaaaag gtgtcgcgag tgagatagag aaacgtatag aacgcttcat
     3241 cgccgctcac attcattcca tagcgtttta acgccagcca gaacatgcca acaatcccca
     3301 gcacgcccgc cgccgccagc atccacaacg aaatccagcc gcgaataatg ccaataaaca
     3361 ggaagatagc gaatgcgatg atgatattgg cgcgagtgcc gccgacaatc atataagtca
     3421 gcaagccaaa ggcgaccgtg ctgacgagga aaaacagcca cgctttgctg tcctggcgca
     3481 gaaagtagac caccagcatc gccgggatga aaaagtaaaa gaagcgtttt aacgccacgc
     3541 cggagacttc actggaaaag atctgactgt aggagttaag ccggaacagc aaaaagccgt
     3601 tgtgcatgaa gaagatgccg acgcttacca gcgcgatacc catcaggatc acccacgtaa
     3661 gattggtctc cacgcggttc atggtaaaca gcggacggcg cggtacatca gcaacgcgtt
     3721 tgcgtaggcg ggttttgtag gtgacatagt aaaccgcgta gaagcagccc gcagaaagca
     3781 acgcctgcaa caagatttct ggcggcgcga caccaacatc aaagcgaaat accagcacgc
     3841 tggtcagcgg gaagccgaag aaaaaggtga gcaaaaacag caatgaaaag aagacattga
     3901 agttaaagtg cacacggcga aactcaaacc aggtcagcgt ggcaataaac agcgtgcaga
     3961 gcagccagac cacaaacagg ccactgaatt gcagcagact catgcgacct ccccggcggc
     4021 aatcgccaac gcccgctgcc agccttgtag atagttaggg ctaaagaagg caatggtgtt
     4081 tttatccgcc gacgccaact ggcgctgcgc ttcacgcaca atatcctcgt tgagatcgtc
     4141 ggtagtaaac agcactggca aatgctgttc cgtcatatcc tgccagaacg gattttccct
     4201 gttaagcaca caaggaatgc ccgcctgaat cagtaagcaa agcgtaccaa tgccctgctg
     4261 gcgggcaaaa ataaagtaac caagatcgca ctgacgaagt agcgccagat aggcgtcaaa
     4321 ttccagtttt tcgctcaaaa tttgcagatt ttcttcgctg aataactcca gccccgcctg
     4381 acgaacttcc tcaatgtacg cttcgttatt aggcggatat cccatcggca ccaccacttt
     4441 taccgtatcg ccaaattgct gatgaacggc acgtaaagcg gcgacatgct cattgctgcg
     4501 gtcgccggag ttccccacca gaatggtcat ttttccttca cgttgccgat cgttcgccat
     4561 cgtattgaga gaagggtcca tccgcgtcgg gaagtacagc tgttctcccc gcacctttgg
     4621 gtgcgttttg gcaaaaaagc tcaaatcgcc gcgggtggca aatacacagc cgactcgctt
     4681 ttgcgccagg cgacgtagtg ggtaaaaaag cttatatctc aagccactgg aaagctcata
     4741 caggtctgcc ccccagatat gccagaaaaa ctggctgggc ttaatgccac cactcagcag
     4801 agccagccac agtgtgggat tgaactgacc gtggaaaaaa aatcgctgct gacggttagc
     4861 ttttgctttc gcgatgaccg cttccgccag cgattttttc ccagggaaaa attgcacaga
     4921 aagcgccgga cagctatcac ttaagccgtc gtccttgcca acaaccataa actcgcgcgc
     4981 gtgctcgctc gtcgcggcca gcgcgtcatt gaaaaaccgc aaaacggttc ggttatggtg
     5041 agggatatcc gatcccagta cgtgaatcag tacagtcatg cccgcctacg ccagagtaaa
     5101 aacacgccac aacaaagaga aaaatagacg atataagttg ccatatatgc ctgcgccgcg
     5161 cccagtgcac catgcgcagg gattagccag tgggcaaata ccatcaataa agtgaactgg
     5221 ctgacttccg ccagaatata aaaccgcagt gacgctttgg cgatcaccag ataaccaaag
     5281 acataagcgc ccacttttaa cacatcaccc actaactgcc aggcaaagag atcgcgcata
     5341 gcggtaaatt tattcgacaa cagcaaccag atagcaaaat cacgcagcaa ccagacggta
     5401 aaactcgctg ccgccactgc cggtaagacg aatttcagcg atttaaccac ttcccgggtg
     5461 atatcgcgct tttccgttag ccgcgacaac gtgggcagca aatatacgct gaacgatgcc
     5521 gtaataaatt gcaggtaggc atcggaaata ctgctcaccc cttgccagat ccccacttca
     5581 tcccagctat actgcgccgc cagcagttta cgcatcatga tgtaagcaac aggcaaggtc
     5641 accgacgtaa tcaacgccat gagcgtaaat ttgctcaact gccctgccag accgttatcc
     5701 cagctgggtt tcagatagct taacgggatg acaccacgtt taatcaacat gatggcggca
     5761 ggaattacca ccagcgcggg aatcagcgcc agacccagca acgccccttc ataaccgccc
     5821 aaacggtaac tgacgtagta cgcgagaacg ccaatcaagc tgccgacaat cagcgataac
     5881 gcattacctg cggcatcgcg aaagcctttc atcagcgcca gtaacaggtt gccccaggcg
     5941 atccccattt gcaccagcgc cactaaacgc accagcccct gatagtcggt attaccaaac
     6001 aatccctggc tgattggcgc agctgccagc acaaaaacca gcgccatcag cgtagagaaa
     6061 ccaagtacca tcgctgatga agtgccgacc acgcggcgca gctgttgcgg attatcatgg
     6121 tactgggcaa cgtatttggt tacaccgtta aagatgccag ccccggcaag cacgccgagc
     6181 acggtaatca actggcggaa atttgccgcc agcccaagcc ccgccggtcc aaatgacacc
     6241 gccagcaact taccgaccag taacccggca ccaatcttga ccagtgtact ggccgccgtc
     6301 cacaaggacg cttttgccaa cgacatatca ggagaagtag ttcaacaaag tcgcaattac
     6361 cgtacgctga ttgacgggcg acaggttgta gaacagcggc aggcgcagca ggcgctcgct
     6421 ctctttggtg gtgtagcgat cttcaccgtg gaactcacca aagtgttccc ccgcagggca
     6481 accgtgcagc ggaatgtaat gaaacaccgc catgatttcc gcttctttca gaaagttaat
     6541 caacgcgctc cggtcatcaa tatcccgcag tttaatgtag aacatatgcg cgttctgcac
     6601 gcagccatcg ggaatcgacg gcagctcgat acgcccggct ttcgccagag gcgctaacgc
     6661 atcgtagtag ttttgccaca gcgccagacg ttgctggttg atacgatccg ctgcttccag
     6721 ttgcgcccac aggtacgcag cttgcagatc ggacatcaaa tagctggagc caatatcgcg
     6781 ccaggtatat ttatcgacct gaccacggaa gaactggctg cggttagtgc ccttttcacg
     6841 gatgatctcg gctcgttcga ttaacgcttt atcgttaatc agcgtcgcgc cgccttcacc
     6901 acccgccgtg tagtttttgg tttcatggaa gctaaagcag ccaatatgac caatggttcc
     6961 cagtgcacgc cctttgtaag tggacatcac gccctgagcg gcatcttcca ccacaaacaa
     7021 attatgcttt ttcgccaacg ccataatggt gtccatttcg caggccacac ccgcgtaatg
     7081 gaccggcacg ataacgcgcg ttttgtcggt gatcgctgct tcaatcaacg tttcgtcgat
     7141 gttcatggtg tccgggcgaa catccacaaa gacgattttc gcgccacgca gcacaaaggc
     7201 attggcggtg gagacaaagg tgtagctcgg catgatcact tcatcgccag gctggatatc
     7261 gagcagcagc gccgccatct ccagcgaagc ggtgcaggac ggcgtcagta acactttggc
     7321 gctgccaaaa cgttgctcca gccactgctg gcagcgacgg gtaaaaccgc catcgccaca
     7381 cagtttgccg ctacccattg ccgactgcat atagtcgagt tcggttccca ccaccggcgg
     7441 tgcgttaaat ggaatcatgt gatcacctgt ataaccagta cgcggtgctt tctacattcg
     7501 caccactttg tatgtatcgt ttaagcgcgg cggtgttgcc catttgggtc gccacccgca
     7561 aagttgtttt accgcgagca tacgcccagt ttagcgccgt ttgcatcagc tcagcacctg
     7621 caccgcgtcc agccagcagg ccaattcgcg catctgtcgc attgagttcc cgtaaagaga
     7681 catagccgcg aatatcgccg gacgccgcac gtagaatcag acattgatga tcaaaggtgc
     7741 cgcgcacggc attttcaatc cactgtgcat aaaagcgacc gctggcgtca ggcgcatacc
     7801 acggcgcacg aaaacggctt tgcgcaaatg cggcgctagc taactgacgt aatgcgggaa
     7861 tatcggtctc ttgtgccacg acagcaccgc tatcactgac attgttcacg ggtagcgcca
     7921 aatcaacttc accttctacc agggagaatc ccagctgttg cagggcatcc agttcccccg
     7981 tatttgatgc cgcaattttg gcctgcaccc gtgaccatgg cgctaaggcg tctgacgtca
     8041 ggagcggtgc ttcagacgta atgcgcacga tggcgctgtt aacaccaaag aaggcgtttt
     8101 cccaggttag tggctcaata ctggcgcgga cgggcacgaa gtaactccag cagatattgg
     8161 ccgtagccag ttttcgctaa tgaactggca gcacgcttca caccctcgtc atcgagccag
     8221 ccgttacgcc aggcgatctc ttccaggcag gcaatcttaa agccctggcg tttttctact
     8281 gtctgcacaa aagtgctggc ctcaatcagg ctgtcgtgag tgccggtatc cagccaagca
     8341 aatccgcgcc cgagcagttc aacggtcagg ttgcccgcct cgaggtacat ctggttgatg
     8401 gaggtaatct ccagttcacc gcgctccgac ggcttcacct gctttgcgta ctccacgact
     8461 ttactgtcgt agaaataaag cccggtcacc gcccagtttg acttcggctg ttttggcttt
     8521 tcttcaagcg agatagcgcg gaaattgtcg tcaaattcca ccacgccaaa gcgttccggg
     8581 tccatcacct gatagccaaa aaccgtcgcc ccttccgtgc gcgccgcaac atgacgcagc
     8641 ttcggactga agcactgacc gaagaagatg ttatcgccca gcaccagaca agaaggttca
     8701 ccattaagga aggtttcacc gatgataaag gcctgcgcca gaccgtccgg gctgggctgt
     8761 tcggcatatt ccagctggat accgaactca ctaccatcgc ccagcaggcg ctggaaataa
     8821 cctttatctt ccggcgtagt gatgatgaga attttgcgga taccggccag catcagcacc
     8881 gacagcggat agtaaatcat tggcttatcg taaatcggca acagttgctt cgatacgccg
     8941 cgcgtaatcg gatgcaatcg ggtaccggaa ccgcccgcca ggataatacc tttcatgttt
     9001 gcctccgctg aaaattagcc tttcagacct aaacgctcac cctgatagct gccgtcctgc
     9061 acctgcttcc accagctttc attagccaga taccactgaa ccgttttacg cattccactt
     9121 tcaaaggttt cctgcggcag ccagccaagt tcacgggcaa ttttcgaagc atcaatggca
     9181 taacgcagat catgccccgg acggtcagcg acaaaggtga tcaggtcacg atagtgcgcc
     9241 acgccgtgcg gcttgttcga agccagttct tccagcagct cgcaaatggt ttccacaaca
     9301 tcgagattct tacgctcgtt gtgaccacca atattatagg tttcaccgac tttcccggtg
     9361 gtcgccacgc aatacagcgc gcgggcgtga tcttccacat acagccagtc acggatttgc
     9421 tgcccgttgc catataccgg cagcggttta cccgccagcg cgttgaggat catcagcggg
     9481 atcagttttt ccggaaagtg gtaaggaccg tagttattcg agcagttggt gatcagcgtc
     9541 ggcagaccgt aggtccgcag ccaggcgcgc accagatggt cgctgctggc ttttgacgcg
     9601 gaataagggc tgctcggcgc atacggcgtg gtttcggtga agaaatcatc cgtcgagtgc
     9661 aggtcaccat atacttcgtc ggtggagata tgatgaaaac ggaacgctga ttttttatct
     9721 tccgtcagcg tattccagta agcccgtgcc gcttcaagca atgtataagt cccgacaatg
     9781 ttggtttcaa taaacgctgc cgggccgtca atagaacggt caacatggct ttcggctgcc
     9841 agatgcatga cacagtctgg ctgatgctca gtgaatacgc gtgccagttc tgcccgatcg
     9901 cagatatcaa ctttctcaaa ggcaaagcgc tcgccttgcg cgaccggtgc cagcgacatc
     9961 aggtttccgg cgtaggtcag cttatcgacc actaccaccg catcgctcgt ttcgttgatg
    10021 atataacgca ccagcgccga gccaataaac ccggcaccac ctgttatcag aatttttctc
    10081 atcagcgcca gactcctttg gcatcgacga catactgctg atggacattg tcgccattga
    10141 taactttgaa ctgactatga tcgaccagca tcaccagcac gtctgccgtt gccagcgcct
    10201 cgtcaagctg cgccagagta caaagcccgg tcagtttttt cggcaactgg tggatgttag
    10261 gctcaacaac cagcgtttcg ccgctgtgcc actgggcgat cagttcagcg atttccatcg
    10321 ccgggctttc gcgcaggtca tcaatattcg gtttaaacgc cagaccaaag caggcgattt
    10381 tcagttcact ggcgcgttta tcggtagcag caaggcagtc ggcaaccgcc gctttcacct
    10441 gatcgataac ccagaacggt ttgtgatcgt taacttcgcg cgcggtacgg ataagccgcg
    10501 cctgctgggg gttctgtgcc acgataaacc acggatcaac agcaatgcag tgaccgccca
    10561 cgccagggcc aggctgaaga atattaacgc gagggtgacg attcgccagg cgaatcagtt
    10621 cccagacgtt aatcccctga tcggcacaaa tcagcgacaa ttcattagca aaagcgatat
    10681 tcacatcgcg gaagctgttt tcggtgagct tacacatttc cgccgtccgc gagttagtga
    10741 cgacacactc accttcgagg aaaattttgt acaattcgct ggcgcgggcc gaacaaaccg
    10801 gcgtcatacc accaatcacg cgatcgtttt taatcagctc gaccataacc tggcccggta
    10861 atacacgttc cgggcagtaa gcaatgttga cgtccgcctg ctcgcccacc tgctgtggga
    10921 aagtcagatc cgggcgcatc tctgctaacc attctgccat cttctcggtt gaccccaccg
    10981 gcgaggtgga ttcaaggatc accagcgcgc cttttttcag cactggcgca atggagcgag
    11041 cagccgattc aacgtaggtc atatctggct catgatcgcc tttaaacggc gtaggtacag
    11101 caatcagcca ggcatccgct tcaactggcg tcgtgctcgc tcgtaaaaaa ccgccttcta
    11161 cggcagtttt tactacgctc gccaaatcag gctcgacgat atggatttcg ccacgattga
    11221 tggtatcaac cgcgtgttgg ttgatatcga caccaatcac ctgtttttgc cgtgaggcaa
    11281 acgctgctgc tgtaggcagc ccgatgtaac ccagtccgat aacagaaatg gtcgcaaaac
    11341 tcatagtgat atccgattat tttttaacgc ttccagaatg cgagagcatg cctgaccatc
    11401 accatacggg ttatgggcgc ggctcatagc ttgatattcg ttttcgtctt ttaaaagacg
    11461 cgtcacttcc tcgacaattc gctgcttatc cgtgcctacc agacgcaccg tacccgccgt
    11521 caccgcttcc ggacgctcag tggtatcgcg catcaccagc accggtttcc ccagcgaagg
    11581 cgcttcttcc tgaatgccgc ctgagtcagt caaaatcagc caggcgtggt tcatcagcca
    11641 gacaaacggt aaatactcct ggggatcgat cagaatgaca tttttcacat gccccagaat
    11701 gcgattgacc ggctctctga cgttcgggtt gagatgcacc ggatagacaa tctggatgtc
    11761 ctggtgcgtg gtggcgatgt ctgccagcgc gtggcagatt tcttcaaagc cacgaccgaa
    11821 actctcacgc ctatgaccgg tcaccagaat catcttttta tcggggtcga taaacgggta
    11881 atttgccgcc agttctgaac gcagcgtgtc gctgctcatc acctggtcac gcacccataa
    11941 cagtgcatca atgactgtat taccggtaat gaagattcgg ctatccgcaa cgttttcacg
    12001 cagcaagttt tgccgggaag tttcggttgg agagaagtga tacatcgcca gatgcccggt
    12061 caatgtacgg ttagcctctt ccggccacgg cgaatagaga tcgcccgtgc gcagaccagc
    12121 ctcaacgtga ccaacaggaa tacgctgata aaacgccgcc aggctggttg ccagcgtcgt
    12181 cgtcgtatcg ccgtgaacca gcacgacgtc tggtttgaac tcggcaagaa taggttttag
    12241 cccttccaga atccgacagg ttatctctgt caggccctgt cctggctgca ttatgttgag
    12301 atcgtagtca ggtacaatgg aaaagagttt cagtacctga tcaagcatct cccgatgctg
    12361 cgcagtgacg caaactttag cctcaaaaaa aggatctttt gccaacgcat gcaccaacgg
    12421 agccatcttg atggcttccg ggcgcgtacc aaatacagtc agtactttca catcgattct
    12481 cttcgaataa gcggcgagcg cctttgcgct caccgcagca gtgttgctat ttcgagcaac
    12541 ggcgggttaa tgcgacacca gccccgatca gccccccgac aatgccccac ataatcatca
    12601 ggaaggcacg acgtgggcta tcgcgtttta ccggttcttc cggcgtacgc aaatagcgat
    12661 aggtctgaaa acgcggatcc agggttggac caacattcag ggtgtttaac atggctcgat
    12721 tctgatcata gtcgagatca aaggccggac cgacggcctg taaattttcc agtcgagcct
    12781 ggagcattgg acgcccaagc aggaacattt ctgaatcagg taattcctcg gcaggcacat
    12841 ctgtcgcact gcgcgaaata ttatgctgct cagcaatttt cagcgcctgc tcaatgctgt
    12901 tcatccggcg gtcgtagatg gctttcgcca cctcttcctg acgcttcacc tgagctttca
    12961 tctggatggt gcgtgccgcc catgcgcctt tcagctcatc attcagatgg ctggctgcac
    13021 gctggctggc aaaagcaaca tactgacgta acaggttatt agcgtcaggc gcggtttcgg
    13081 caataagctt cacgctgtca ttgaccgcgc gagtaaagtc tccggggata aactggatgt
    13141 tgttaatcat ttcatccagc aacgccgcat cggctttgct gttgcccacc atccgctgtt
    13201 tgtaatagtc ggtttgcagc cagaactctc tgcgggtatc ccacgaggcc agttgcataa
    13261 caaactcttt ataggcttcg tccatgaccg atggttgatc ggcagaagcc atgtttgaac
    13321 ggacatccag gttacgcaaa aattgctgct gcgagtaata tccccccagc atattcaccg
    13381 ttggacgatc ggtaatcgcc gtcgagctcc actcctgacg agcaaaaaaa gtatacgcca
    13441 gcgcgattaa cgcaaacgcc agccccatgc caataatcca tagcttccca gcccacaagg
    13501 tacgaaacaa cccacgaata tccagttcat tttcagcgtc ttcggccggt ttcccaggca
    13561 ttggttgtgt catcacatcc tcatttattt ggttaaattg gggctgccac cacgatttct
    13621 acgcagtctg cgttttacgc gcttaataaa gcgagcaact ttccaggcac gcttaatgca
    13681 atatccatag aggaagaatg ctagcaaaaa gagcaccagc atgacccact ccgggacaaa
    13741 atgagaatat tctgccagca cgccaatgga agcgagcagt gctgcggcaa gggtaatcag
    13801 cacaaacgcc tgacgggaag taaacccggc acgcatgatc aaatggtgaa tatgctgacg
    13861 gtcaggagag aatgggctca tgcctttacg caggcgacgg tacataatcg ccaccatatc
    13921 cattagcgga atggcgatta tccacaaagc ggtaaccggg ctgatgggat gggttttgcc
    13981 ctgggtcgtt tcgagcagga tccagataac ggtaaaacca atcagcgtac tgcccgcatc
    14041 acccataaag actttgtagc ggcgacccag gataccaagg ttaagcatga tgtatggcag
    14101 gatggcggcg atcatcgcaa agcaccagat tgcgaggctg gtttgcccgt cgaaccacaa
    14161 aatcataccg attgctgcaa acgagacgca ggacaacccg cccagcaagc catcaatgcc
    14221 atcaaccatg ttgaacgcat taatggccgc ccagacggca aatagcgtca ggaagtaacc
    14281 aaacggtccg agcaccatct cccaggagcc aaagatataa cccaggctac tgagataaag
    14341 tttgccgaac accatcataa caatgccaac agcggcctgt atggtggcac ggatttttac
    14401 gctgatatca aaacgatcat ccagcgcgcc aatgaaaaca agcacaccgg cacaagcgag
    14461 atagagagat gcatgcggaa tatagtaatc gacaattccg aacgtgaagc aaatccctgc
    14521 gtaaaccgaa atccccccaa cgagaggtat caatccctgg tgacgtttgc ggaagtttgg
    14581 tttatccact aaaccgactt tttttgccac cttacgggca aaaaacagaa acagtgtcgt
    14641 gaataaaaaa atactgatga gatcagtact cactgtcagt aaattcacaa tgcatgctct
    14701 cagagaaaat tattagcaga agtataacca cgaagacctt tattcagaag ggaaagtccg
    14761 ttccgaaccc accagctctg tgcaaaaatt aaccatttga tatgcaaatt gcataacatg
    14821 ccagcaaatt ccaggaattt tcctaacatc gccagcgcgg cattaagatt acagatataa
    14881 aagcaaaacg ccacgtaaac acgtggcgtt tttggcataa gacaaattta tgagcgtttc
    14941 atcatttcga agaaatcgtc attggtcttg gtcatggcca gtttattaat gaggaattcc
    15001 attgcatcga tttcgcccat cgggtgaatg attttgcgca ggatccacat tttctgcagt
    15061 tcttcctgag tcgtgagcag ctcttcttta cgggtaccgg aacggttgta gtcgatagcc
    15121 gggaagacgc gtttttcagc gatcttacga gagaggtgca gttccatgtt gcctgtacct
    15181 ttaaactctt cgtaaataac ttcgtccatt ttagaaccgg tatcgataag cgccgtcgcg
    15241 ataatggtca ggctgccgcc ctcttccacg ttacgcgccg caccaaagaa gcgtttcgga
    15301 cgatgaaggg cattggcgtc cacaccaccg gtcaacactt tacctgacgc cggaacaaca
    15361 gtgttgtaag cgcgcgccag acgagtgatg gagtcgagca gaatgataac gtctttcttg
    15421 tgctcaacca ggcgtttggc cttctcgatc accatttccg caacctgaac gtggcgagat
    15481 gcgggttcgt caaaggtaga agcaacaact tcacctttta ccagacgctg catctcggtt
    15541 acttcttccg gacgttcgtc gatcagcaga accatcagca cacaatccgg gtggttgtaa
    15601 gcaatgctct gagcaatgtt ctgcaacagc atagttttac cggctttcgg cggtgccaca
    15661 atcagaccac gctgaccacg accgataggt gatgccagat ccagtacgcg agcagttaaa
    15721 tcttcagtag aaccgttacc acgttccata cgcagacgag agtttgcgtg cagcggggtt
    15781 aagttctcaa agaggatttt gttgcgggcg ttttcaggtt tgtcgaagtt aacttcgtta
    15841 actttcagca gcgcaaaata gcgttcacct tctttcggcg ggcgaatctt accagagatg
    15901 gtatcaccag tgcggaggtt gaaacggcgg atttggctag gggaaacgta gatgtcatca
    15961 ggaccggcga ggtaggagct gtctgcggaa cggaggaaac caaatccatc ctgcaatatc
    16021 tccagtacgc catcaccaaa gatatcttcg ccactctttg cgtgctgctt caggatggca
    16081 aaaataatgt cctgcttacg catgcgagcc aggttttcca gccccatatt ttcgccgaga
    16141 gtgatcagct cagaaaccgg cgtattcttt aattcggtaa gattcatagt ggtgtgagtt
    16201 cttaaacttg gggtaaatct cgaacttaat gttgtgaatg gtatggcagg atcatccatg
    16261 cctgtttacg gtcatcgact catgtctttt cgctgcctgg tcacaggaga gtacgcagaa
    16321 ctgaaacgac aagacggatt gagtgacgag ccagaaatct gttcacttcg catttaagat
    16381 aaaacgggaa gcgttggaaa ttacaagatt caaacttaat aaggtatgtt taatacgaag
    16441 tcaacactaa gttagcatga ctcacgccgg gcgtccagtt tttagcgacg gggcacccga
    16501 acatgaaatt cccttacgcc aggttagcgt cgaggaactc tttcaactga cctttagaca
    16561 gtgcgcccac tttggttgcc gccacttcac cgtttttgaa cagcagcaga gtcgggatac
    16621 cacggatgcc atatttcggc gcagtgccag ggttttgatc gatgttcagt tttgcaacgg
    16681 tcagtttgcc ctgatattcg tcagcgattt catccagaat cggggcgatc attttgcacg
    16741 gaccgcacca ctctgcccag aaatcgacga ggatcgcccc gtccgctttg agtacatccg
    16801 tgtcaaaact gtcgtcagtc aggtgaataa ttttatcgct catatataac tccacaggaa
    16861 taagcctggc gtgttggttt cgttgttggt gtaacattaa ccaactaaag gttgacttta
    16921 tttcaccgga tacgctttcg taaagcaata gtaagctgat attctaccac actatgagca
    16981 aaacacattt aacagaacag aagttttccg acttcgccct gcatccgaag gttgtagaag
    17041 cccttgaaaa aaaagggttt cataactgta cgcccattca ggcactggcc cttccgctga
    17101 cgctggcggg tcgtgacgta gccgggcagg cgcaaaccgg taccgggaaa acgatggcgt
    17161 ttcttacgtc aacgtttcat tatcttctct ctcatcctgc gattgccgat cgcaaggtga
    17221 atcaaccgcg tgccttaatt atggcaccga cgcgtgaact tgccgtgcag atccacgccg
    17281 acgcggaacc gctggcggaa gctaccggcc tgaagctggg cctggcttac ggtggtgatg
    17341 gctatgacaa acagctgaaa gtgctggaaa gcggcgttga cattctgatt ggcaccacgg
    17401 gtcgtttaat tgactacgcc aagcagaacc acattaacct cggtgccatt caggtagtgg
    17461 tactggacga agccgatcgc atgtacgatc taggctttat taaagatatc cgctggctgt
    17521 tccgccgtat gccgcctgca aaccagcgcc tcaacatgct gttctccgcc acgctttcgt
    17581 accgggtacg tgaactggcg ttcgagcaga tgaacaatgc cgaatatatt gaagtggaac
    17641 cggaacagaa aacgggccac cgtattaaag aagagctttt ctacccttct aacgaagaaa
    17701 aaatgcgttt gctgcaaacg ctgatcgaag aagagtggcc agacagagcg attattttcg
    17761 ccaataccaa acaccgttgt gaagagatct ggggccacct ggcggcagat ggtcatcgtg
    17821 tcggtttgtt gacaggcgat gtcgcgcaga aaaaacgtct gcgtattctt gatgaattta
    17881 cccgtggcga tctggatatt ctggttgcca ccgacgttgc cgcgcgtggt ttgcatattc
    17941 cggcagtgac gcacgtcttt aactacgatt tacccgatga ctgtgaagat tacgttcacc
    18001 gtattggtcg tacaggtcgc gcaggcgcaa gcggtcactc tatcagcctg gcgtgtgaag
    18061 agtatgcatt gaatttgcct gctattgaga cctatattgg tcactcaatt ccggtaagca
    18121 aatacaatcc ggacgcattg atgaccgatc tgccaaaacc gctgcgcctc acgcgcccgc
    18181 gcacaggcaa tggtccgcgt cgtactggcg ctccgcgtaa tcgtcgtcgt tcaggttaaa
    18241 taaaatgtca tactcaaaat cattcaaact gtatcaagat ggcaagagaa tgaatcccga
    18301 tgagcttact gtagtaagtg attcgggtga aagaatgcag ccaacacaga gacagattga
    18361 aggatgaaga gtatgggttc cacctcgtcg ctgtatgcag ccattgatct cggttcgaat
    18421 agttttcata tgctggttgt gcgcgaggtg gctggaagca tccagacgct gacgcgaatt
    18481 aaacgcaaag tgcgtctggc tgctggcctg aatagcgaaa atgacctctc taatgaagca
    18541 atggagcgcg gttggcaatg tctgcgcctg tttgctgaac gtctgcaaga tatccctccc
    18601 tcgcaaattc gcgttgtcgc tacggcgaca ttacgacttg ccgtcaatgc gggtgatttt
    18661 attgccaaag cgcaggaaat cctcggttgt ccggtacagg tgatcagcgg tgaagaggaa
    18721 gcgcgtctga tttatcaggg tgttgctcac accactggtg gtgccgatca gcgcctggtg
    18781 gtggatatag gcggtgccag tactgaactg gtaaccggca cgggtgcaca aaccacctcg
    18841 ttgttcagcc tgtcgatggg ctgcgtcacc tggctggaac gctattttgc cgatcgtaat
    18901 ctggggcaag aaaattttga tgctgcagaa aaagcggcac gcgaagtgtt acgtccggtt
    18961 gccgatgaat tacggtatca cggctggaaa gtgtgcgttg gtgcttccgg caccgtgcag
    19021 gcgttacagg aaatcatgat ggcacagggg atggatgaac gcattaccct ggaaaagttg
    19081 cagcaattga aacagcgagc cattcattgc ggtcggctgg aggaactgga gattgacggg
    19141 ctgacgctgg aacgtgcgtt agtgttcccg agtggtctgg cgatcctgat cgccattttt
    19201 accgaactga atattcagtg tatgaccctg gcgggcggtg cgctgcgtga aggcctggtc
    19261 tacggcatgt tgcatctggc cgtcgagcag gatattcgca gccgtacact gcgtaatatt
    19321 caacgccgct ttatgatcga tattgatcag gcacagcgcg tagccaaagt tgcggctaac
    19381 ttcttcgatc aggtggaaaa cgaatggcat cttgaagcaa taagccgcga tttgctcatc
    19441 agcgcctgtc agcttcatga aatcggcctg agcgttgact tcaaacaagc tccgcaacac
    19501 gctgcttatc tggtgcgtaa cctggatctt cccggtttta cccccgcaca gaagaaactg
    19561 ctggcgacgc tactgctcaa ccagactaac ccggtcgatc tctcatcgct gcatcagcaa
    19621 aatgccgtac caccgcgcgt cgcagaacaa ctctgccgtt tactccgcct ggctatcatt
    19681 tttgccagcc gtcgccgtga cgatctcgtg ccagagatga cattacaggc taaccatgaa
    19741 ctgttgacct tgacgcttcc gcaaggttgg ctaacccaac atccgctggg taaagagatt
    19801 attgctcagg aaaaccagtg gcagagctat gtccactggc cgctggaagt gcattaatga
    19861 gtaagtgccg gatgcgatgc tgacgcatct tctccggcct tgattatttc cctcgttttg
    19921 ccgccatcat cgctttcaga ttcgccagat ggctttgccc tttctgcatc cgttcttcgg
    19981 cactgaccac tttgcgctcc tgttcccaaa tcagatcatc ctgcggcagc tccagcaaaa
    20041 agcggctcgg ctccgggcgc accagttcgc cgtactggcg gcgttcttta cacagcgtaa
    20101 aggtcaattc cttctgggcg cgggtaatgc cgacataggc cagccgccgc tcctcatcga
    20161 tattatcttc atcgatactg ctctggtgcg gcaaaaaccc ttcttccata ccgaccatgt
    20221 agacataagg aaactccagc ccttttgacg cgtggagagt catcagttgc acctgatcca
    20281 gctcttcttc actctcacca cgctccatca tgtcgcgtaa agtaaagcgc gtcaccacct
    20341 gggtgagcgt catcggctca tccagttcgc tgccttccag catctccgtc atccagctaa
    20401 acagttggtt gacgttcttc atgcgcattt cggcagcttt cgggctgggc gatgtttcgt
    20461 acagccagga ttcataatcc atgccgtgga tcagatcgcg caccgcggca atcggctccc
    20521 gctccgccag acgctggatt tctgccaacc agtgagtgaa gcgggtcaat gcttcataac
    20581 cacgtccgct aagcgtctga ctcaggccca tatcaaagct ggcggtaaac atgcttttat
    20641 tgcgcgtcat cgcccactca cccagctttt tcagcgtcgc cgggccaatc tctcgcttcg
    20701 gcgtgttaac gatacgcaga aatgcgctgt catcgtccgg gttagtcagc acgcgcagat
    20761 aagccagcaa gtccttgatt tcaggacgag agaaaaacga cgtaccacca gaaattttgt
    20821 acgggatgcg gttttgcatc aggaattttt caaacacccg cgactgatgg ttaccgcgat
    20881 aaagaatagc gtaatctttg tactgcgttt tattgacgaa gtgatgcgca atcagttcac
    20941 cagtgacgcg ctcggcttca tgttcttcgt tattcgcgct taaaactttt agctctgtgc
    21001 cgtaacccag ttcggagaac agacgctttt caaagacgtg cgggttattg gcgatcagga
    21061 tgttcgccgc tttcaaaata cgtccggaag agcggtagtt ctgctcaagc ttaatcacct
    21121 tcagcgccgg aaaatcctgg ctcagcagca ccaggttttg cggacgtgcg ccgcgccagg
    21181 agtagatcga ctggtcatcg tcacccacca cggtaaagcg cgcgcggctg cccaccagca
    21241 gtttcaccag ttcatactgg ctggtgttgg tgtcctgata ttcgtccacc agcaggtagc
    21301 gaattttgtt ctgccagcgc tcgcggactt cttcattgcg ttgcagaagt agcgtcggca
    21361 ataaaatcag atcatcgaag tcgagcacat tacaggcttt caggtgtgca tcatacagcc
    21421 cataacaatg ggcaaaaata cggtcccgct cgccaatcgc actggctgcc gcctgggacg
    21481 gtgttttgag atcgttcttc cagttagaga tggtcgaaat caactgttgc aggagaactt
    21541 tgtcatcttc aatcagccct tcggtcaact ctttgagcaa cgccagctga tcggtatcgt
    21601 caaacagcga aaagttcgcc ttcatcccca gcgccgcata ctcacgcttg atgatatcca
    21661 gccccagcgt gtggaaggtt gagatcatca gcccacgcgc ctctttgcga cccagcgtct
    21721 gtccgacacg ctctttcatc tcgcgcgccg ccttattggt aaaggtcacc gccgcgatat
    21781 gtcgcgcctg ataaccgcac ccgcggatca gatgggcgat tttattggtg ataacacgag
    21841 ttttaccgga acccgcaccc gccagcacca ggcaggggcc ggtaacgaat tcgacagctt
    21901 gttgttggcc ggggtttaga cgcataggaa aatcgctcaa tgaaagtcgg gaggggaaaa
    21961 taacagcgtg gtagtatagc cagcctcatc cttaccactc aaggcacgat catggcaaaa
    22021 acagcagcag cactgcatat ccttgtaaaa gaagagaaac tggctctgga tcttctcgtg
    22081 cagattaaga acggtgccga tttcggcaag ctggcgaaga aacactccat ttgcccatca
    22141 ggcaaacgcg gcggtgattt aggtgaattc cgccagggtc agatggttcc ggcgttcgat
    22201 aaagttgtgt tctcttgtcc ggtactggag ccaaccggcc cgctgcacac ccagttcggt
    22261 taccacatca ttaaggtgct gtaccgtaac taatagcaag gccttctccg ggagaaggcc
    22321 ttgaactctg aatcgctatt aacccgcaac agcaatacgt ttcatatccg tcatatagcc
    22381 gcgcagtttc ttacctactt gctcaatcgc atggctgcga atcgcttcat ttacatcacg
    22441 cagctgcgcg ttatctaccg caccttccgg aatagcttta cccaaatcgc ccggttgcag
    22501 ttctgccata aacggtttca gcagcggcac acaagcgtaa gagaacagat agttaccgta
    22561 ctcggcagta tcagagataa ccacgttcat ttcatacaga cgcttacggg cgatggtgtt
    22621 ggcaatcagc ggcagttcgt gcagtgattc atagtaagca gattcttcga tgatgccgga
    22681 atcaaccatg gtttcgaacg ccagttcaac gcccgctttc accatcgcga tcatcagtac
    22741 gcctttatcg aagtactcct gctcgccgat tttgccttca tactgcggcg cggtttcgaa
    22801 ggcggttttg caggtctctt cacgccaggt cagcagtttc ttatcatcgt tggcccagtc
    22861 cgccatcata ccggaggaga attcgccgga gatgatgtcg tccatatgtt tctggaacag
    22921 cggtgccatg atctctttca gctgttcaga caacgcgtaa gcacgcagtt tcgccgggtt
    22981 agagagacgg tccatcatca gtgtgatacc gccctgcttc agcgcttcgg tgatggtttc
    23041 ccaaccgaac tgaattagtt tttctgcgta tgccggatcg gtgccctctt ccaccagctt
    23101 gtcgaagcac agcagcgagc cagcctgcaa cataccgcac aggatggttt gctcacccat
    23161 caggtcagat ttcacttccg caacgaagga cgattccagc acgcccgcac ggtgaccacc
    23221 ggttgctgcc gcccatgctt tagcgatcgc catgccttcg cctttcggat cgttttccgg
    23281 gtgaacggca atcagcgtcg gtacgccgaa tccacgtttg tactcttcac gcacttcggt
    23341 gccagggcat ttcggcgcaa ccatgactac ggtgatgtct ttacggatct gctcgcccac
    23401 ttcgacgatg ttgaaaccgt gcgagtaacc cagcgccgcg ccgtctttca tcagtggctg
    23461 tacggtgcgc actacatcag agtgctgctt gtccggcgtc aggttaacca ccagatccgc
    23521 ctgtgggatc agttcttcgt aagcacccac tttaaaacca ttttcggtcg ctttacgcca
    23581 ggacgcgcgc ttctcggcaa tcgcttcttt acgcagagcg taggagatat cgagaccaga
    23641 atcacgcatg ttcagaccct ggttcagacc ttgtgcgcca cagccgacga tgactacttt
    23701 tttaccctga aggtagctcg cgccatcggc gaattcatcg cgccccataa agcgacattt
    23761 gcccagctgt gccagctgct gacgcagatt cagtgtattg aagtagttag ccatggtgat
    23821 tcctcgtgat gttgtgcttc ttattgttgc gtttgcgaga ttgaattcac tatatgacag
    23881 gaaatttatt gcggaaattg atatattcac aacgtcatat tgcaatttct gcaacgtcaa
    23941 cattgagggc tgcgccattg gatttacgcg atctgaaaac cttcctgcat ctggcggaaa
    24001 gccgccattt tggccgcagc gcgcgggcga tgcacgttag cccgtccacg ctctcacggc
    24061 agattcagcg cctggaagaa gatctcggcc agccgctgtt tgtgcgcgat aaccgcacgg
    24121 tgacgctgac tgaagcgggc gaagaactgc gcgttttcgc ccagcaaacg ctgttgcagt
    24181 atcagcagct gcgccacacc atcgaccagc aagggccgtc gctctatggc gaattacata
    24241 tcttctgctc ggtgaccgcc gcttacagcc atctgccgcc gattctggat cgcttccgcg
    24301 cggaacaccc gtcggtggag atcaaactca ctaccggtga tgcggcagat gcaatggaaa
    24361 aagtcgtcac aggtgaagct gatctggcga ttgccgggaa gccagagact ctgcccggcg
    24421 cggtggcgtt ttcgatgctg gagaatctgg cagtggtgtt gattgcaccc gcattgccct
    24481 gcccggtgcg taatcaggtt tctgtcgaaa agccggactg gtcaacggta ccgtttatca
    24541 tggccgatca ggggccggtg cgccgccgca ttgagttgtg gttccgacgc aataaaatca
    24601 gtaacccgat gatttacgcc acggttggcg ggcatgaagc gatggtatcg atggtggcgc
    24661 tcggctgtgg cgtggcattg ttgccggaag tggtgctgga aaacagcccc gaaccggtgc
    24721 gtaaccgcgt gatgatttta gagcgcagcg atgagaaaac gccgtttgag ctgggcgtgt
    24781 gtgcgcagaa aaagcggcta catgagccgc taattgaggc attctggaag attttgccga
    24841 accattaacc cgccaaaaag aacctgaacg ccgggttatt ggtttcgtcg tggcaatcgt
    24901 agcccagctc attcagccgg gtttcgaaat ccggttcatg gtcgccaagt tcgaacgccg
    24961 ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg gaacaaagaa atgttccagt
    25021 gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg tgattccggg aattcgaagc
    25081 tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc gaccatatag cgcacatgca
    25141 acttcgccat ttcgtcgtcg gagagatcca ccacgctgta gccgccgtcg ttgagcatct
    25201 gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg cacgccgaca aagatgcagg
    25261 cgtttttggc atcggcaaaa cggtagttga actcggtgac cgatcgcccg ccaagcagtt
    25321 ggcagaattt gaggaagctg cctttttctt ccggaatggt caccgccaac aacgcttcac
    25381 gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag gccgtggaag ttcacgttgg
    25441 caccggaaag aatatgcgcc agccgttcgc cgcgaatgtt gtgctgggcg atatattttt
    25501 tcattcccgc cagcgccagc gcgccagagg gttccgccac cgcacgcaca tcttcgaata
    25561 aatccttcat cgccgcacag atcgcatcgc tatcgacggt gatgatgtcg tcgagatact
    25621 cctggcataa acggaaggtt tcgtcaccga tgcgttttac cgcgacacct tcggcgaaca
    25681 gtcccacgcg tggcagatca accggatgac ccgcatccag cgctgctttc aggcaggcgg
    25741 agtcttccgc ttctacggcg atcactttga tttgcggcat cagttgtttg atcagcaccg
    25801 ctacgcccgc cgccagaccg ccgccgccga ctggcacaaa tacgcggtcg agatgggcgt
    25861 cctgctggag cagttccagc gccagcgtgc cttgcccggc aatcaccatc ggatgatcga
    25921 acggcggcac ccaggtgaac ccctgctgct gtgacagttc gatcgcttta gctttcgctt
    25981 cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa tccacgtacc gcatcgactt
    26041 tgatgtcggc ggtggcggtt ggcataacga tcagggcctt cacgcctaac cgcgcagaag
    26101 aaaacgcgac gccctgcgcg tggttacccg cagaagcagt gatcacgccg tgcgcttttt
    26161 gttcttccgt caggcccgcc atcatggcgt atgcgccgcg cagcttaaag ctgtgcactg
    26221 gctggcgatc ttcgcgcttc accagaatga cgttatcaag acgcgacgac agtttttcca
    26281 ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg cgcgcgcagc actgctctta
    26341 aatattcggc accttccgga gcaccggaca ggggttgcga gtcagccatt attaaccccc
    26401 cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg gttgccaggc tggcgtaggc
    26461 gcgcagggca aaggaaacct gacgttcacg gtttttcggc gtccaggctt tgtcaccccg
    26521 agcttcctgc gcttcacgac gcgccgccag ttcggcatcg cttacctgta actgaatgcc
    26581 acggttcggg atgtcgatag cgatcaggtc accatcttca atcagaccaa tgctgccgcc
    26641 gcttgccgct tccggtgaga cgtggccgat ggaaagacca gaggtgccgc cagagaaacg
    26701 accgtcggtg atcagcgcac aggctttacc gagacccatt gatttcagga agctggttgg
    26761 gtagagcatt tcctgcatgc cagggccgcc tttcgggcct tcatagcgga tcaccaccac
    26821 atcgcccgcg acaactttgc caccgaggat cgcttctacc gcgtcgtcct ggctttcgta
    26881 cactttcgcc gggccggtga atttgaggat gctgtcatcg acgcccgcgg ttttcacgat
    26941 gcagccgttt tcagcaaaat taccgtagag caccgccagg ccgccgtctt tgctgtaggc
    27001 gtgttccagc gagcggatac agccgtttgc gcgatcgtca tcgagagaat cccaacggca
    27061 atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg cctgcgcgga acatattttt
    27121 taccgcgtca tcctgggtca gcataacgtc gtattgttcc agagtttgcg gcaacgtcag
    27181 gccaagtacg tttttcacat cacggttcag taaccccgcg cgatccagtt caccgagaat
    27241 accgataaca ccaccagcac ggtgaacatc ttccatatgg tatttttggg tgctcggcgc
    27301 aactttgcat aactgcggga ctttacgcga aagtttgtcg atatcactca tggtgaagtc
    27361 gatttccgct tcctgcgccg ccgccagcag gtgaagtacc gtgttggtag agccgcccat
    27421 cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg ctagcaatat tgcgcggcag
    27481 tgcactttcg tcgttttgct cgtagtaacg tttggtcaat tcaacaatgc gtttaccggc
    27541 attgaggaac aactgcttac ggtccgcgtg ggttgccagc agcgagccgt tgcccggctg
    27601 cgacaggccc agcgcttcgg tcaggcagtt cattgagtta gcggtaaaca tcccggagca
    27661 ggagccgcag gtcggacacg cggaacgttc aacctgatcg ctctgggagt cagaaacttt
    27721 cgggtctgcg ccctggatca tcgcatcaac cagatcgagt ttgattatct gatcggacag
    27781 tttggttttc ccggcctcca tcgggccgcc ggaaacaaag atcaccggaa tattcaggcg
    27841 cagggaagcc atcagcatcc ccggggtgat tttgtcgcag ttagagatgc agaccatggc
    27901 gtcggcgcag tgggcgttga ccatatactc aacggaatca gcgatcagtt cgcgagatgg
    27961 aagtgaataa agcatccccc cgtggcccat ggcaatccca tcatccaccg caatggtgtt
    28021 gaactctttg gcaacgccgc cagccgcttc aatttgttcg gcgaccagtt taccgagatc
    28081 gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag ttcacaaccg cgataatcgg
    28141 cttgccaaaa tcggcgtcgg tcattccggt ggcgcgccac agcgcacgag cacccgccat
    28201 attacgacca tgagtggtgg tggcggaacg gtacttaggc atactttatt tactcccagt
    28261 gtctgtctcg taaatgggac ggtgcgtgcc gtcccatttt ttgtatttat tgattaactt
    28321 gatctaacca gccccattta tcttcggttt cgccagtgaa gaggccgaag aaggcttgct
    28381 gaatgcgttt ggtaaccggg ccacaacggc cttcgccaat ctgaataccg tctacgctgc
    28441 gcactggcgt gatttctgcc gccgtaccgg acataaagac ttcatccgcc aggtacaggg
    28501 attcgcgcga cagcacctgc tcacgcactt caattcccag ctctttcgcc agtttgatga
    28561 tggcatcacg ggtaataccc ggcagcgcgg aggaggtgaa cggtggggtg aacagcacac
    28621 catctttcac ttcaaacagg ttttcgcctg cgccttcaga gatataaccg ttcacatcca
    28681 gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct acccaccagc agggaagaga
    28741 gatagttacc accggctttt gccgcagttg ggatggtgtt tggtgctgcg cggttccagg
    28801 aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc gcccagatac gctccccacg
    28861 ggaaagcggc gataatcaca tcggttgagt atcccgctgg cgggtttact cccatgccaa
    28921 catcaccgac gaagatcagc ggacggatat aggcgctggt gagattgttt ttgcggatca
    28981 cgtcacgaca agcttccatc agctcatcaa tgctcagtga aaccgggaag cgatagattt
    29041 tggcggagtc atgcagacgc tgcatatgct cacgatggcg gaatacaacc ggtcctttgt
    29101 gtgagtcgta gcaacggatg ccttcaaaaa ccgaggtgcc atagtgcagc gcgtgcgaca
    29161 tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc attgaaccaa atgtaatcag
    29221 ctttcttcgt ggtcattttt atattccttt tgcgctcagg cgcggatttg ttgtgatgtg
    29281 gttgtgctct ggcagatggc aacgtgtgcg acgtccacca gtttatttaa ctgactaaac
    29341 agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga tatttatatt ttgtgcatcg
    29401 ctggcggcag ccatattcat tgagcagacg tggaaaccac gatgacgcac cacgcgtaaa
    29461 acacgttcta aggtttccgg attgaagcga gccgatacat tgacctgatg ttgcatcatg
    29521 ataatttctc caacatttct gaattactgg cacctggcgg caccagcggc cagacgttct
    29581 caagttcgtc gattgagaca tgaagcaggt atggcccatc actgttcagc atggtgtcga
    29641 gtgccgcttc aacctggtct ttacgggtga tgtgttggcc agggatgccg aaggcgctgg
    29701 ctaacatgag gaaatcgggg ttatcggtaa gggtggtttc gctgtatcgt tcctgaaaaa
    29761 acagttgctg ccattgtcga accatcccta accgttggtt atcgagtaag acgattttca
    29821 acggtaactg cttgcgtttt acggtgccca gctcttgcac attcatcatg aaagagccgt
    29881 caccggagat acagacaacg gtatcgttcg gtcgcgcgac ttgtgcgcca actgccgccg
    29941 gtaaaccaaa acccatggta cctaagccgc tggaggtgat gaaattttcc gggcgagtgt
    30001 gggcgatgtg ctgcgcagcc cacatctggt gctgccccac atctgtggtc acgacgcaat
    30061 ccgcaggttt acgatccgac agttgtttta acaacaacgg agcgtagata gcgtcaccgg
    30121 gatggtcgta acgccaggaa tgttcatcac gcagctgcgc gcagtgttgc tgccagtcat
    30181 tgatatttaa cggctgctgt aatgctggta acagagcatt taaatcacct tgtaatgcca
    30241 catgtgcctg acgcagcttg ttcatttctg ccgggtcgat atccatatgg ataacactgg
    30301 cgtgtggtgc gaaggtgttc agtttgccgg tcacccggtc atcaaaacgt gcacccacgg
    30361 cgatcagcag gtcacactcc tgcaccgcga agtttgccgc tttggttccg tgcatcccca
    30421 gcatgcccag atagtacgga taatctgctt ctactgcgcc cagccctttc agcgtacagg
    30481 tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc cggaactgcc tgcgccatcc
    30541 ccacgccacc gccaacgtac agcatcggtt tttgcgcttt tgccagcatc tggcgcgctt
    30601 gctcgacttc ggcatgtggg aaagtcactt cgttttcaac ggtggtgaac cacggttcca
    30661 gatcgccgct ggctaactgg atatcttttg ggatatcgac cagaaccgga ccaggacgac
    30721 ctgagctggc aacgtcgaat gcttcaacca tgatgcgcgg caactcttcc agagactgca
    30781 ccagaaagct gtgcttggta caggctaacg acaatcccag gacatccact tcctgaaatg
    30841 cgtcggtgcc gataaacggt gcggacacct gaccagtgat ggcaacgaca gggatggaat
    30901 ctaacagtgc gtccgcaagc ccggttatca ggttggttgc gcccggacca gacgtggcga
    30961 tacatacgcc agttttaccg gtagcacggg cataaccgat agccgccatt gccgcaccct
    31021 gctcatgtcg gcacagcaag tgctccacgc cgccgtcata caatgcatcg taaaccggca
    31081 taattgcgcc acccggataa ccgaaaacgg tgttcacacc ctgttcccgc aacgcatgta
    31141 ccacccactg tgcgccattc atagttagtt ccccgtcctg aatcttgaga aacagaattt
    31201 tgtgctgtta ttcattgtct gctcctcggt tatgttttta aggtcaaaaa aacccccgga
    31261 cctttcggtg cgggggtctt agttcgttaa ggcttgatct ctaagccttt cctcgtccaa
    31321 gtgcagcccc gcacggtggg ataataatca ccaccacgct aatcacgacc aggctaatca
    31381 ctcgtagaag ggctgtcatt ttgtcttttc ttgcatcttg ttcgaaggaa tgcctaaaga
    31441 gttaccatag attttgcgaa tagcacaaga tatttttggg cctattttta tgatggcttt
    31501 aataatttca atagaaattg ttgtttttta aaaagaaaat agtgagtgaa aaaattcaat
    31561 ttcagtataa atgttgtccc cttcctcgta gttcatcctt tgtccgctgc atttttgcga
    31621 gcatcattcc accgccagaa caactcaact tcacttgcac aaataaacag gaatcagcca
    31681 ctaaaattca cgaattagcg ggctacagca gcgatatctt atgcacataa tggtgactca
    31741 aggagggttt atgtcactgt caattgttca tacccgcgca gccctggaag taaatgcgcc
    31801 cccaatcact gttgaggtac atatcagtaa aggtctaccc ggcttaacga tggtgggctt
    31861 accagaaaca acggtgaaag aagctcgcga tcgcgtgcgc agcgccatta tcaatagcgg
    31921 atatgaatat ccggcgaaaa aaatcaccat caaccttgct ccagccgatc tgccaaaaga
    31981 agggggacga tatgatttac ctatcgccat tgcgttgctg gcggcctcag aacagcttac
    32041 agccaataag ttagatgaat atgaattagt cggagaacta gcgcttacag gcgctctgcg
    32101 tggcgttccc ggtgcaatct ccagtgcaac tgaagctatt aagtcgggca gaaaaattat
    32161 cgtcgcgaaa gataacgaag atgaagtggg gctaattaac ggtgaaggat gcctgatagc
    32221 cgatcatctg caggctgtct gtgcgtttct ggaaggtaag aacactctcg aacgcccgaa
    32281 accaactgat gcagtatccc gggcgctaca acatgatctc agtgatgttg tcggtcagga
    32341 gcaaggaaaa cgaggactgg aaattaccgc cgctggtggg cacaaccttt tactgattgg
    32401 gccgccggga acaggtaaaa caatgctcgc cagccgtatt aatggcctgt tgccagattt
    32461 aagcaatgaa gaggcactgg agagcgctgc gatattaagt ctggtaaatg ctgaatcagt
    32521 acaaaaacaa tggcggcagc gcccgttccg ctcacctcat cacagtgcgt cgttaactgc
    32581 gatggtgggc ggtggtgcaa ttccagggcc aggtgaaatt tcgttggcgc ataacggcgt
    32641 gctttttctt gatgagctac ctgaatttga acggcgtaca ctggatgcct tgcgagagcc
    32701 gattgaatcc gggcagatcc atctttcacg cacacgagca aaaataacct atccagcccg
    32761 tttccagctt gttgcggcga tgaatcccag ccctaccgga cattatcagg gaaaccataa
    32821 ccgctgcacg ccagaacaga cattacgtta tctcaaccgg ctctcggggc cctttctcga
    32881 ccgcttcgat ctctcactgg agatcccatt accacccccc ggcattttga gtaaaacggt
    32941 agtgccggga gaaagcagcg ccaccgttaa acaacgcgta atggccgcca gagagcgcca
    33001 atttaagcgg cagaataaac tgaatgcctg gctggatagt ccggaaatac gccaattctg
    33061 caaacttgag agcaaagatg cgatgtggtt ggaagaaacg ctgatccatc tggggttatc
    33121 gattcgtgcc tagcagcggt tactgaaagt tgcacgaacc attgctgata ttgatcagtc
    33181 tgacattatc acacgtcagc atttgcagga ggcagttagc tatcgtgcga ttgaccgttt
    33241 gctcatccat ctgcaaaaac tactgacata aaaaaagggc atttcgccct ttttattaat
    33301 cgtcagaatc ggtgtagtct tcagcacctt caacctgcgg tttaccgccg gaaagggtgt
    33361 gaaaacgttt tggacgctta atacgcgtca tatacttgga ccacacgcgt tctgcttctg
    33421 tcactggctc acgttcgcca cggcatactg ctacgaagag tttctcttcc tcggtaaccg
    33481 gctcgcgttt gccaagatcc aactcattga aggcataacc atgacgctca agcagttgtg
    33541 cctctttgat ggtgaaatca ccatgacgag agaatccacg tggataatgt ttattgtcga
    33601 aatatcgatt agtcgtcgta aagctttccg ccatcctgca cgctcctaat tctttgaccg
    33661 agctagttat ggcgcggagt attagttacg cttgacagag tgtaaaacaa aacatttaaa
    33721 tcataacgac aaataatttt gcggagagca ctgtggatac ggaattgtta aaaactttcc
    33781 tggaagttag ccgaacgcgt cactttggtc gagcggctga atcgctctat ctgacccagt
    33841 cagcagtgag ctttcgaatc agacaactgg aaaatcaact gggtgtgaac cttttcaccc
    33901 gccacagaaa caatatccgt ttaaccgctg ccggtgaaaa actactgcct tatgcagaaa
    33961 cgctcatgag cacctggcag gccgcccgca aggaggtggc gcatacctca cgacataacg
    34021 agttttctat cggtgccagc gcctcgttgt gggaatgtat gcttaatcag tggttgggac
    34081 gcttgtatca aaatcaggat gcccatacag gcttacagtt cgaagcgcga attgcccaac
    34141 ggcagtctct ggtaaaacag ctgcatgaac gccagcttga tcttcttatt accactgaag
    34201 cgcccaaaat ggacgaattt agtagtcagt tgctggggta tttcacttta gcgctttata
    34261 ccagtgcccc ttctaaacta aagggagatc ttaactatct gcgactggag tgggggccag
    34321 attttcaaca gcatgaggca ggtttgatcg gtgctgacga agttcccatt ctgacaacca
    34381 gttctgctga actggcacag caacagattg cgatgcttaa tggttgcacc tggctacccg
    34441 tcagctgggc gcgtaaaaaa ggcggcctgc ataccgttgt cgatagcaca acactttcac
    34501 ggccgcttta tgccatatgg ctgcaaaata gcgataaaaa tgcgttgatt cgcgatcttt
    34561 tgaaaattaa cgtgctggat gaagtgtatt aatctgaatg gctggcaagg atgccggtag
    34621 aaggatttac ttcggagagg attatttcag ataaaaaaaa atccttagct ttcgctaagg
    34681 atgatttctg gcaggggcgg agagactcga actcccaaca cccggttttg gagaccggtg
    34741 ctctaccaat tgaactacgc ccctaattag
//
LOCUS       DADLSA010000029        34363 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.029, whole genome shotgun sequence.
ACCESSION   DADLSA010000029 DADLSA010000000
VERSION     DADLSA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.029"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..329)
                     /locus_tag="JN691_001400"
     CDS             complement(<1..329)
                     /locus_tag="JN691_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242879.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYLQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQ"
     gene            699..1649
                     /gene="corA"
                     /locus_tag="JN691_001401"
     CDS             699..1649
                     /gene="corA"
                     /locus_tag="JN691_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAY7242880.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1690..2070)
                     /locus_tag="JN691_001402"
     CDS             complement(1690..2070)
                     /locus_tag="JN691_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HAY7242881.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     TYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2084..2500)
                     /locus_tag="JN691_001403"
                     /pseudo
     CDS             complement(2084..2500)
                     /locus_tag="JN691_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2559..3449)
                     /gene="rarD"
                     /locus_tag="JN691_001404"
     CDS             complement(2559..3449)
                     /gene="rarD"
                     /locus_tag="JN691_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAY7242882.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3501..3968)
                     /locus_tag="JN691_001405"
     CDS             complement(3501..3968)
                     /locus_tag="JN691_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAY7242883.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASVLDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4133..5002
                     /gene="pldA"
                     /locus_tag="JN691_001406"
     CDS             4133..5002
                     /gene="pldA"
                     /locus_tag="JN691_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAY7242884.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5135..6964
                     /gene="recQ"
                     /locus_tag="JN691_001407"
     CDS             5135..6964
                     /gene="recQ"
                     /locus_tag="JN691_001407"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAY7242885.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7028..7648
                     /gene="rhtC"
                     /locus_tag="JN691_001408"
     CDS             7028..7648
                     /gene="rhtC"
                     /locus_tag="JN691_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HAY7242886.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7710..8330)
                     /gene="rhtB"
                     /locus_tag="JN691_001409"
     CDS             complement(7710..8330)
                     /gene="rhtB"
                     /locus_tag="JN691_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAY7242887.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8441..9463
                     /gene="pldB"
                     /locus_tag="JN691_001410"
     CDS             8441..9463
                     /gene="pldB"
                     /locus_tag="JN691_001410"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAY7242888.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNIRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9471..10271
                     /gene="yigL"
                     /locus_tag="JN691_001411"
     CDS             9471..10271
                     /gene="yigL"
                     /locus_tag="JN691_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HAY7242889.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMCFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10347..11246
                     /gene="bioP"
                     /locus_tag="JN691_001412"
     CDS             10347..11246
                     /gene="bioP"
                     /locus_tag="JN691_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAY7242890.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11134..12087)
                     /gene="metR"
                     /locus_tag="JN691_001413"
     CDS             complement(11134..12087)
                     /gene="metR"
                     /locus_tag="JN691_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAY7242891.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNCPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12323..14584
                     /gene="metE"
                     /locus_tag="JN691_001414"
     CDS             12323..14584
                     /gene="metE"
                     /locus_tag="JN691_001414"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAY7242892.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14623..15438)
                     /locus_tag="JN691_001415"
     CDS             complement(14623..15438)
                     /locus_tag="JN691_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY7242893.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYSGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15700..16461
                     /gene="udp"
                     /locus_tag="JN691_001416"
     CDS             15700..16461
                     /gene="udp"
                     /locus_tag="JN691_001416"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAY7242894.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16602..18029
                     /gene="rmuC"
                     /locus_tag="JN691_001417"
     CDS             16602..18029
                     /gene="rmuC"
                     /locus_tag="JN691_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAY7242895.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18124..18879
                     /gene="ubiE"
                     /locus_tag="JN691_001418"
     CDS             18124..18879
                     /gene="ubiE"
                     /locus_tag="JN691_001418"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAY7242896.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18893..19498
                     /gene="ubiJ"
                     /locus_tag="JN691_001419"
     CDS             18893..19498
                     /gene="ubiJ"
                     /locus_tag="JN691_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAY7242897.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19495..21135
                     /gene="ubiB"
                     /locus_tag="JN691_001420"
     CDS             19495..21135
                     /gene="ubiB"
                     /locus_tag="JN691_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAY7242898.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21214..21483
                     /gene="tatA"
                     /locus_tag="JN691_001421"
     CDS             21214..21483
                     /gene="tatA"
                     /locus_tag="JN691_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAY7242899.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21487..21987
                     /gene="tatB"
                     /locus_tag="JN691_001422"
     CDS             21487..21987
                     /gene="tatB"
                     /locus_tag="JN691_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAY7242900.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPEKASDE
                     AHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKTAAPSP
                     SSSDKP"
     gene            21990..22766
                     /gene="tatC"
                     /locus_tag="JN691_001423"
     CDS             21990..22766
                     /gene="tatC"
                     /locus_tag="JN691_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAY7242901.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22808..23590
                     /gene="tatD"
                     /locus_tag="JN691_001424"
     CDS             22808..23590
                     /gene="tatD"
                     /locus_tag="JN691_001424"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAY7242902.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23587..24075)
                     /gene="rfaH"
                     /locus_tag="JN691_001425"
     CDS             complement(23587..24075)
                     /gene="rfaH"
                     /locus_tag="JN691_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAY7242903.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24242..25735
                     /gene="ubiD"
                     /locus_tag="JN691_001426"
     CDS             24242..25735
                     /gene="ubiD"
                     /locus_tag="JN691_001426"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAY7242904.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25781..26482
                     /gene="fre"
                     /locus_tag="JN691_001427"
     CDS             25781..26482
                     /gene="fre"
                     /locus_tag="JN691_001427"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAY7242905.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEADWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26674..27837)
                     /gene="fadA"
                     /locus_tag="JN691_001428"
     CDS             complement(26674..27837)
                     /gene="fadA"
                     /locus_tag="JN691_001428"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAY7242906.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWSCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGMGQGIATVFERV"
     gene            complement(27847..30036)
                     /gene="fadB"
                     /locus_tag="JN691_001429"
     CDS             complement(27847..30036)
                     /gene="fadB"
                     /locus_tag="JN691_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HAY7242907.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30226..31557
                     /gene="pepQ"
                     /locus_tag="JN691_001430"
     CDS             30226..31557
                     /gene="pepQ"
                     /locus_tag="JN691_001430"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAY7242908.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31557..32171
                     /locus_tag="JN691_001431"
     CDS             31557..32171
                     /locus_tag="JN691_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAY7242909.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32210..33661
                     /gene="trkH"
                     /locus_tag="JN691_001432"
     CDS             32210..33661
                     /gene="trkH"
                     /locus_tag="JN691_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAY7242910.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAIDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33673..34218
                     /gene="hemG"
                     /locus_tag="JN691_001433"
     CDS             33673..34218
                     /gene="hemG"
                     /locus_tag="JN691_001433"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAY7242911.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8075 a         8696 c         9290 g         8302 t
ORIGIN      
        1 tgctgaccgt tttcgatgta caaaccgagc ggcgcatagc tttcatcata gatgccgccg
       61 ttcatcgcca tctgcacctg accctgacta ttaatatccg ccagcagagc atgtaacgtt
      121 ccccacgctt cgccattggc tttttgcaag tacattttca cccgctctgt ttgaggatta
      181 acggtatacg cctgtacggt cagcgtcgga tctgagagtg cgcaatcatc agcggcaaca
      241 gcaaacaagg gaagtaaggt gagggtgaga aaaatccgtt tgagattcaa ggtgatcatt
      301 cctttaccaa tgagtagccg atgcgccatt ataggtcctg gatgtgggat ttttttatcc
      361 tgttagcgac cttgacgagt accaaaaagc gcgaagttca actattgttc tgtggtgttc
      421 tgttgcgtgt tgacggcaaa attttgctgg cgtaacatgc gcgcacgatc actctaagag
      481 gacattcgcc ttggacacac ccagtagata ctggctcact atcctgtcat ccaggatcaa
      541 ctcctaaggc tatccctttt tgctgatagc cttagcggtt gtcagcgacc tcaatttttc
      601 ccgtcgcgct gagtcaggct gtttaatggt ctgaaaccca atttgtttct gtgtgcccac
      661 cgaactgtcc gatattttaa gcattgggag tcccggtcat gctgagcgca tttcaactgg
      721 aaaataaccg actgacccgg ctggaagtcg aagagtcaca accccttgta aatgcagtat
      781 ggattgatct tgtcgaaccg gacgacgacg agcgactgcg cgtacaatct gaacttggcc
      841 agagcctggc aacccgcccg gaactggaag acatcgaagc atcggcacgt ttctttgaag
      901 acgacgacgg cctgcatatt cactccttct tcttctttga agatgcggaa gatcacgccg
      961 gtaactccac tgtggcattt accatccgtg atggtcgtct gtttactctg cgtgagcgtg
     1021 aactgcccgc ttttcgtctg tatcgtatgc gtgcccgtag ccagtcgatg gtagacggta
     1081 acgcctacga gttgctgctg gatctgttcg aaaccaaaat cgaacagttg gcagatgaaa
     1141 ttgaaaatat ctatagcgac ctggagcagt tgagccgggt gattatggaa gggcatcagg
     1201 gcgatgagta cgacgaggcg ctctccacgc tggcggaact ggaagatatc ggctggaaag
     1261 ttcgcctgtg tctgatggat acccagcgcg ctctcaactt cctagtgcgt aaagcgcgtt
     1321 taccgggtgg gcaactggag caggcgcgtg aaatcctgcg cgatatcgaa tccctgctgc
     1381 cgcataacga atccctgttc cagaaggtga acttcctgat gcaggcggca atgggtttta
     1441 tcaacatcga gcagaaccgc atcatcaaaa ttttctcggt ggtatccgtc gtgttcctgc
     1501 cgccaacgct ggttgcttcc agctacggga tgaactttga gtttatgcca gaactgaagt
     1561 ggagtttcgg ctaccctggc gcgattatct ttatgatcct cgcgggcctg gcaccgtatc
     1621 tgtactttaa gcggaagaac tggttgtaaa aagaaggcgg tggcttagcc aggctaagcc
     1681 tcctgttatt caaaggctcc aggtatttaa cccttttacc tctttctcat aaaaccattt
     1741 gttcgtgtta acagcaacat aggttgctac ggcaattccc agaatgttaa cgccaattaa
     1801 tgcaccaaca aatagagata gaatgccaag taatagaact ataattgctt ttttccataa
     1861 gcccagaaca aataaataga tccaggaaca gaagaaagca atgaaattca tttgaatagt
     1921 taatcgttgt cttactttta atgctttaaa tgctgcttta tattctggtg ttgctcccca
     1981 gaatccagga aagccatgtt gatcataaaa attaaatcgg tatttccatt tttcacttaa
     2041 tgaatcatcg ttcatatatt ccttactcat aaatactcca taactattgt tttgatgaat
     2101 cagtaggtgc aagcattagc atactgaaag tggaaaaata acaaatcaaa aaaatcatcg
     2161 aaccattgcc tgaacaggca aaatcttcgg ctatcattgt gatgatagag atgatatata
     2221 ctgctaatgt accaaaaaca taaatttata tatatatgaa accactatca cggagtcgtt
     2281 ggcaattcat gttgatgacg agataatgga gtgcgatggt agagactata acaagaaaac
     2341 ctgcttcccc atcgttaaaa aagataataa gaaaggcaaa aatgaaattt attaaaataa
     2401 atgaaaatat atatcggcgt ctggaaatct taccgttaga tgttgggata aacatacgta
     2461 acataaattt tacatccttg tgtgagtctc ctgtcagcat ggcaatatgc ccactctcat
     2521 acaagagtcg gcatattttt tcagaatata tttatttttt atttggacgt tctacgctgc
     2581 gtataaatcg cgtccatcac aaaaattgcc agcgccaccc aaataaaggt gaaagtcacc
     2641 atcttatcgg cacccggttt ttcaccataa aacgtcacag ccagcaggaa catcagcgtc
     2701 gggccaatgt actgaaaaaa gcctaacgtt gagagacgca ggcgcgtggc agcggcagta
     2761 aaacacaaca gcggtacggt agtgacaata ccggcggcga tcagcagtaa attcagcgac
     2821 atcgggtttt gccccatatg gctggttgaa ctgtcggcaa tagcaaacag gtaaatcgcc
     2881 gccacgggca gcagccacat ggtttcaatt aacatgccgg tttgtgcttc taccgcaatc
     2941 ttcttgcgca ccaggccgta gaaggcaaaa ctaaatgcca gccccagcgc gataataggt
     3001 agcgaaccaa aagtccacag ctggaccaac acgccacata tcgccagaat caccgccagc
     3061 cattgcatcc ggcggaatcg ctcgccgagg aaaatcatcc ccagcacaat gttcaccagc
     3121 gggttaataa agtaaccaag gctcgcttcc agcatatggt gattgttcac cgcccagata
     3181 aacagtagcc agttgccacc aatcagcacg gcagagactg ccagcataaa aattttctgt
     3241 ggcgtctgaa tcagcgtttt taaataggac cactggcggc aaatgctcat cagcaccacc
     3301 ataaagaaaa acgaccagat cacgcgatgc gtcaggatct catcggcggg cacgtagtaa
     3361 atcagcttga agtatgctgg cgcaataccc caaataaaat aagcggcaag agcgagtaat
     3421 acgccctgcc gcgtttgttt tgcatccatc gggaatactc atttttaatt ggtaacagca
     3481 gtttacctgc ttttatgtct tcaacctacc atataggtgg cggtggcact ggcaatataa
     3541 agctgttctt cattgtgtaa ttcaacgcgg gcgacggcga ctttattgcc tgcacgcaac
     3601 aggctactgg tagcagtaaa acgctcgccc ctgcctgggc gcagataatc aacgcgaaga
     3661 tcaatggtcc ccatccgcga tagccgctgg cgtagttcat cttcactgat ggtttcgtgg
     3721 cgggttaagg tacttcccac gcacaccaga ccggcggcga catccagcac cgacgcaatg
     3781 actccgccgt gcaaaatgct ttgcgcccag ttacccacca tcatcggctg atttttaaag
     3841 gccagctgtg cgaactcttt ttcgtaacgc tccagttcca tccccaatgc gcggttaaat
     3901 ggcatgtgat aaacaaacat ctcacccact aatttcaggg cttgttcagc ggtcagtacg
     3961 gcagacatat catccttaca cttcattggt taatgaaatg ttgattttat gcttctttgt
     4021 tgttggtttc tactttagga agggataact aacggcaatg gagttaagta tgtaaaatag
     4081 cccgcagaaa aacattcacc ttatcaataa ttcgttacgg agaacacgac cgatgcggac
     4141 tctgcagggc tggttgttgc cggtgtttat gttgcctatg gcagtatatg cacaagaggc
     4201 aacggtgaaa gaggtgcatg acgcgccagc ggtgcgtggc agtattatcg ccaatatgct
     4261 gcaggagcat gacaatccgt tcacgctcta tccttatgac accaactacc tcatctacac
     4321 ccaaaccagc gatctgaata aagaagcgat tgccagttac gactgggcgg aaaatgcgcg
     4381 taaggatgaa gtaaagtttc agttgagcct ggcatttccg ctgtggcgtg ggattttagg
     4441 cccaaactcg gtgttgggtg cgtcttatac gcaaaaatcc tggtggcaac tgtccaatag
     4501 cgaagagtct tcaccgtttc gtgaaaccaa ctacgaaccg caattgttcc tcggttttgc
     4561 caccgattac cgttttgcag gttggacgct gcgcgatgtg gagatggggt ataaccacga
     4621 ctctaacggg cgttcagacc cgacctcccg cagctggaac cgcctttata ctcgcctgat
     4681 ggcagaaaac ggtaactggc tggtagaagt gaagccgtgg tatgtggtgg gtaatactga
     4741 cgataacccg gatatcacca aatatatggg ttactaccag cttaaaatcg gctatcacct
     4801 cggtgatgcg gtgctcagtg cgaaaggaca gtacaactgg aacaccggct acggcggcgc
     4861 ggagttaggc ttaagttacc cgatcaccaa acatgtgcgc ctttatactc aggtttacag
     4921 cggctatggc gaatcgctca tcgactataa cttcaaccag acccgtgtcg gtgtaggggt
     4981 tatgctaaac gacttgtttt gatgaacggt tgagtggttg gcaaatctgg aatccagcat
     5041 ccaggattac cctctcagag actaaaagca ttgcagtttc tcgcgcaggc gctgaaaata
     5101 gcgcctgttt ttatttcagg caatcggggt gaatgtggcg caggcggaag tgttgaatct
     5161 ggagtccgga gctaaacagg ttttacaaga aacctttggc taccaacagt ttcgccccgg
     5221 ccaggaagaa attatcgaca ctgtgctttc cggtcgcgat tgcctggttg ttatgcccac
     5281 tggtggcgga aaatcccttt gctatcaaat tcctgcctta ttgctaaacg gccttaccgt
     5341 ggttgtttca ccgctgattt cgttgatgaa agatcaggtg gatcaactac aggccaacgg
     5401 cgtggcggcg gcgtgcctta actcgacgca aacccgcgaa cagcaacttg aagtgatgac
     5461 aggctgccgc accgggcaaa ttcgcttact gtatatcgcc ccggaacgcc tgatgctgga
     5521 taactttctt gagcatctgg cgcactggaa tccggtgtta ttagccgtcg atgaagcgca
     5581 ctgtatctca caatggggcc acgatttccg cccggaatat gccgcgctcg gtcagttgcg
     5641 ccagcggttc ccgacgctgc cgtttatggc gctgaccgcc acagccgacg acaccacgcg
     5701 ccaggatatc gtgcgcctgc tggggctgaa cgatccgctg attcaaatca gcagttttga
     5761 ccgtccgaat attcgctaca tgctgatgga gaaattcaaa ccgctcgatc agttgatgcg
     5821 ctacgtgcag gaacagcgcg gtaagtctgg cattatctac tgcaacagcc gggcgaaagt
     5881 agaagacacc gctgcgcgcc tgcaaagcaa gggtattagc gcggcggcct atcatgccgg
     5941 gctggaaaat aatgtccgcg ccgacgtgca ggagaaattc cagcgcgatg atctgcaaat
     6001 tgtggtggcg acggtggcgt tcggcatggg catcaataaa ccaaacgttc gcttcgtggt
     6061 ccactttgat attccgcgca atatcgaatc ctattatcag gaaaccggtc gcgccgggcg
     6121 tgatggcctg cctgcggaag cgatgctgtt ttacgatccg gctgatatgg cgtggctgcg
     6181 ccgttgtctg gaagagaagc cgcaggggca gttgcaggat atcgagcgcc acaaactcaa
     6241 tgcgatgggc gcgtttgccg aagcgcaaac ttgccgtcgt ctggtattgc tgaactattt
     6301 tggcgaaggg cgtcaggagc cgtgcgggaa ctgcgatatc tgcctcgatc cgccgaaaca
     6361 gtacgacggt tcaaccgatg ctcagattgc cctttccacc attggtcgtg tgaatcagcg
     6421 gtttgggatg ggttatgtgg tggaagtgat tcgtggtgct aataaccagc gtatccgcga
     6481 ctatggtcat gacaaactga aagtctatgg tatgggccgt gataaaagcc atgaacattg
     6541 ggtgagcgtg atccgccagc tgattcatct cggcctggtg acgcaaaata ttgcccagca
     6601 ttctgcccta caactgacag aggccgcgcg cccggtgctg cgcggcgaat cctctttgca
     6661 acttgccgtg ccgcgtatcg tggcgctcaa accgaaagcg atgcagaaat cgttcggcgg
     6721 caactatgat cgcaaactgt tcgccaaatt acgcaaactg cgtaaatcga ttgccgatga
     6781 aagtaatgtc ccgccgtacg tggtgtttaa cgacgcaacc ttgattgaga tggctgaaca
     6841 gatgccgatc accgccagcg aaatgctcag cgttaacggc gttgggatgc gcaagctgga
     6901 acgctttggt aaaccgttta tggcgctgat tcgtgcgcat gtcgatggcg acgacgaaga
     6961 gtagtcagca gcataaaaaa gtgccagtat gaagactccg taaacgtttc ccccgcgagt
     7021 caaatgtatg ttgatgttat ttctcaccgt cgccatggtg cacattgtgg cgcttatgag
     7081 ccccggtccc gatttctttt ttgtctctca gaccgctgtc agtcgttccc gtaaagaagc
     7141 gatgatgggc gtgctgggca ttacctgcgg cgtaatggtt tgggctggga ttgcgttgct
     7201 tggcctgcat ttgattatcg aaaaaatggc ctggctgcat acgctgatta tggtgggcgg
     7261 tggcctgtat ctctgctgga tgggttacca gatgctacgc ggtgcactga aaaaagaggc
     7321 tgtatctgcg cctgcaccac aggttgagct ggcgaaaagc gggcgcagtt tcctgaaagg
     7381 cttactgacc aatctcgcca atccgaaagc gattatctat tttggttcgg tattctcatt
     7441 gtttgtcggc gataacgttg gcactaccga gcgctggggc attttcgcgt tgattattgt
     7501 cgaaacactg gcgtggttta ccgtcgttgc cagtctgttt gccctgccgc aaatgcgccg
     7561 tggttatcaa cgtctggcga agtggattga tggttttgcc ggggcgttat ttgccggatt
     7621 tggcattcat ttgattattt cgcggtgatg ccagacgcgt cttcagagta agtcggataa
     7681 ggcgtttacg ccgcatccga cattattttt cacgcatgcc tcgccgatgc taacagcgct
     7741 cccaccagca taaacaacga gccgaaaatc ttattcagcg ccttcatctg ctttggtcct
     7801 ttaatccata gagcaatcct ttgagcaagg gtggcgtaac cgatcatcac aataatatcg
     7861 accacaatag tggtgacgcc gagcacgata tactgcatca gttgcggctg ttgcggcatg
     7921 atgaattgcg gaaatagcgc cgccagaaac acaatacttt tgggattggt gagattcaca
     7981 aaaactgcgc gctggaacaa atgtcgacgc gattgagtag aggccagcga tttaagatca
     8041 attgcgccag cggcgcgcca ctgctggatt cccagccaaa tcaagtaagc cgcgcctgcc
     8101 cactttaaca cttcaaacgc aatcactgag cgggaaaata gcgtccccaa ccccacgcca
     8161 accagcacaa tatgaatcgc cagtccggtc tgaagcccag caatagacgc caccgcgccg
     8221 cgataaccgt ggttgagcga ggtggtcata gtgttgattg caccagagcc tggcgacagg
     8281 cttaaaatga tcgatgtcag caggtaggca aaccaccatt ctaaggtcat gatgaactcc
     8341 cggtgtgtct atttttgtgc cacaatacgc tactgtcgca gcgttgtgtc aggcacgctt
     8401 aaaaaaacga ttttacgtgg tttaagaggc agattacccg atgtttcagc agcaaaaaga
     8461 ctgggaaaca agagaaaacg cgtttgctgc ttttaccatg ggaccgctga ctgatttctg
     8521 gcgccagcgt gatgaagcag agtttactgg tgtggatgac attccggtgc gctttgtccg
     8581 ttttcgcgca cagcaccatg accgggtggt agtcatctgc ccggggcgta ttgagagcta
     8641 cgtaaaatat gcggaactgg cctacgacct gttccatttg gggtttgatg tcttaatcat
     8701 cgaccatcgc gggcagggac gttccggtcg cctgttagcc gatccgcatc tcgggcatgt
     8761 taatcgcttt aatgattatg ttgatgatct ggcggcattc tggcagcagg aggttcagcc
     8821 cggtccgtgg cgtaaacgct atatactggc acattcgatg ggcggtgcga tctccacatt
     8881 atttctgcaa cgccatccag gtgtatgtga cgccattgcg ctaactgcgc caatgtttgg
     8941 gatcgtgatt cgtatgccgt catttatggc acggcagatc ctcaactggg ccgaagcgca
     9001 tccacgtttc cgtgatggct atgcaatagg caccgggcgc tggcgcgcgt tgccgtttgc
     9061 tatcaacgta ctgacccaca gcagacagcg atatcgacgt aacatacgct tctatgctga
     9121 tgacccaacg attcgcgtcg gtgggccgac ctaccattgg gtacgcgaaa gtattctggc
     9181 tggcgaacag gtgttagccg gtgcgggtga tgacgccacg ccaacgcttc tcttacaagc
     9241 tgaagaggaa cgcgtggtgg ataaccgcat gcacgaccgt ttttgtgaac tccgcaccgc
     9301 cgcgggccat cctgtcgaag gaggacggcc gttggtaata aaaggtgctt accatgagat
     9361 cctttttgaa aaggacgcaa tgcgctcagt cgcgctccac gccatcgttg attttttcaa
     9421 caggcataac tcacccagcg gaaaccgctc tacagaggtt taaatttctt atgtaccagg
     9481 ttgttgcgtc tgatttagat ggcacgttac tttctcccga ccatacgtta tccccttacg
     9541 ccaaagaaac tctgaagctg ctcaccgcgc gcggcatcaa ctttgtgttt gcgaccggtc
     9601 gtcaccacgt tgatgtgggg caaattcgcg ataatctgga gattaagtct tacatgatta
     9661 cctccaatgg tgcgcgcgtt cacgatctgg atggcaatct gatttttgct cataacctgg
     9721 atcgcgacat tgccagcgat ctgtttggcg tagtcaacga caatccggac atcattacta
     9781 acgtttatcg cgacgacgaa tggtttatga atcgccatcg cccggaagag atgtgctttt
     9841 ttaaagaagc ggtgttcaaa tatgcgctgt atgagccggg attactggag ccggaaggcg
     9901 tcagcaaagt gtttttcacc tgcgattccc atgaacaact gctgccgctg gagcaggcga
     9961 ttaacgctcg ctggggcgat cgcgtcaacg tcagtttctc taccttaacc tgtctggaag
    10021 tgatggcggg cggcgtgtcg aaaggccatg cgctggaagc agtggcgaag aaactgggct
    10081 acagcctgaa ggattgtatt gcgtttggtg acgggatgaa cgacgccgaa atgctgtcga
    10141 tggcggggaa aggctgcatt atgggcagtg cgcaccagcg tctgaaagat ctgcatccgg
    10201 agctggaagt tattggtact aatgccgacg acgcggtgcc gcattatctg cgtaaactct
    10261 atttatcgta atcgttcttt atttggtcag ttgtcaacct gatacttcgc tacaatggat
    10321 actcgttaat caaagagttt tccattgtgg cgctacttat catcaccacg attctgtggg
    10381 ccttctcctt tagcttttat ggcgagtacc ttgcggggca cgtcgatagc tattttgcgg
    10441 tgctggtgcg cgttggcctg gcggcactcg tttttctgcc gtttctgcgt acccgtggca
    10501 atagcctgaa aacggtcggc ctgtatatgc tggtgggcgc gatgcagctc ggcgtgatgt
    10561 atatgctgag tttccgcgct tatctctacc tgacggtctc cgaattgttg ctgttcaccg
    10621 tactgacgcc gctctacatc acgctgattt atgacatcat gagtaagcgc cgtctgcgct
    10681 ggggctatgc ttttagcgcc ttgctggcgg tgattggtgc cgggattatt cgctatgatc
    10741 aggtcaccga ccatttctgg actggcttgt tgctggtgca actctccaat atcacttttg
    10801 ccatcggcat ggtgggttac aaacgcctga tggaaactcg cccgatgccg cagcataacg
    10861 cctttgcgtg gttctatctt ggcgcgttcc tggtggcggt aattgcatgg ttcttgctgg
    10921 gaaatgcgca gaaaatgccg caaaccacgt tgcaatgggg cattctggtg tttcttggcg
    10981 tggtggcttc cgggattggc tactttatgt ggaactacgg cgcgacgcag gtggacgccg
    11041 gaacgctggg cattatgaat aatatgcacg ttccggcagg gctgctggta aacctggcta
    11101 tctggcacca acagccgcac tggccaacgt ttattacagg cgcgctggtg atcctggcct
    11161 cactgtgggt gcatcgtaag tgggtcgctc cgcgctcttc acaaacggca gatgatcgca
    11221 ggcgtgattg cgcgctgagc gaataaacgc ttccgtaact ggctgacgct gctcgccatc
    11281 gcgcacggcg gcgtacagtc ggctccacaa gccttcgccc agggttttgg tcaccaccag
    11341 accctggcgc tcaaaactct ctactaccca atgcggtagc gcggcaatac ccatccgcgc
    11401 ggcaaccatt tgaatcaaca ataaggtgtt atcgacgctt ttcagtgacg ggctgacgcc
    11461 tgccggctga agaaaatgcc gccagacatc cagtcgacta cgctgcaccg gataaattaa
    11521 tagcgtctcg ctggcgagat cttccggtgt aatacgcgtt tttgccgcca gtggatggtc
    11581 aggtgctaac accaggcgta cttcataatc gaacatcggc gaataatgca ggccactgcg
    11641 cggcaggata tcggaggtca tcaccagatc cagctcgccc tgttgcaacg cgggctgcgg
    11701 gtcaaacgtc acgcccgatt taaaatccat ctctacctgc gggcagttct tatggaaatt
    11761 ttctaaagcg ggtgtcagcc actgaataca gctatggcac tcaatggcaa tgcgcagacg
    11821 cgtctgctgc ggttcattgc aggcttgcag tgcctggctg atttgcggca gcacctggtt
    11881 tgccagttgc aacaggattt ctccctgcgg tgtaaagcgt agcggctggc tcttacgcac
    11941 aaatagccgg aagccaaggc gttgttccag atcgctaaac tggtgagaca gggcggattg
    12001 cgtctgatgc aacgtcgccg cagcggctgc gagcgagccg cagttccgca acgcttgtag
    12061 cgttttcagg tgttttactt cgatcatgaa agtccttcac ttcggcatga ataatttgcg
    12121 cttgaggaat atacagtaac cgccaattat ggatgtgtaa acatctggac ggctaaaatc
    12181 cttcgtcttt taaatttatg gtgcgttggc tgcgtttctc caccccggtc acttacttca
    12241 gtaagctccc ggcgatgaat aaacttgccg ccttccctaa attcaaaatc cataggattt
    12301 acatataatt agaggaagaa aaatgacaat attgaatcac accctcggtt tccctcgcgt
    12361 tggcctgcgt cgcgagctga aaaaagcgca agaaagttat tgggcgggga actccacgcg
    12421 tgaagaactg ctgacagtag ggcgtgaact gcgtgctcgt cactgggatc aacaaaagca
    12481 agcgggtatc gacctgctgc cggtgggcga ttttgcctgg tacgatcatg tactgaccac
    12541 cagtttgctg ctgggtaatg ttccgccacg tcatcagaac aaagacggtt cggtagatat
    12601 cgacaccctg ttccgtattg gtcgtggacg tgcgccgact ggcgaacctg cggcggcagc
    12661 ggaaatgacc aaatggttta acaccaacta tcactacatg gtgccggagt tcgttaaagg
    12721 ccaacagttc aaactgacct ggacgcagct gctggaggaa gtggacgagg cgctggcgct
    12781 gggccacaac gttaagcctg tgctgctggg gccggttact tacctgtggc tggggaaagt
    12841 gaaaggtgaa cagtttgacc gccttagcct gctgaacgac attctgccgg tttatcagca
    12901 agtgctggca gaactggcga aacgcggcat cgagtgggta cagattgatg aacccgcgct
    12961 ggtactggaa ctaccacagg cgtggctgga cgcatacaaa cccgcttacg acgcgctcca
    13021 gggacaggtg aaactgctgc tgaccaccta ttttgaaggc gtaacgccaa atctcgacac
    13081 gattactgcg ctgcctgttc aggggctgca tgtggacctt gtacatggta aagatgacgt
    13141 tgttgaactg cacaagcgcc tgccttctga ctggctgctg tctgcgggtc tgatcaatgg
    13201 tcgtaacgtc tggcgcgccg atcttactga gaaatatgcg caaattaagg acattgtcgg
    13261 caaacgtgat ttgtgggtgg catcttcctg ctcgttgctg cacagcccca tcgacctgag
    13321 cgtggaaacg cgtcttgatg cagaagtgaa aagctggttt gccttcgccc tacaaaaatg
    13381 ccatgaactg gcactgctgc gcgatgcgct gaacagtggt gacacggcag ctctggcaga
    13441 gtggagcgcc ccaattcagg cgcgtcgtca ctctactcgt gtacataatc cggcggtaga
    13501 aaagcgtctg gcggcgatca ccgcccagga cagccagcgt gcgaatgtct atgaagtgcg
    13561 tgctgaagcc cagcgtgcgc gttttaaact gccagcgtgg ccgaccacca cgattggttc
    13621 cttcccgcaa accacggaaa ttcgtaccct gcgtctggat ttcaaaaagg gcaatctcga
    13681 cgccaacaac taccgcacgg gcattgcgga acatatcaag caggccattg ttgagcagga
    13741 acgtttggga ctggatgtgc tggtacatgg cgaggccgag cgtaatgaca tggtggaata
    13801 ctttggcgag cacctcgacg gatttgtctt tacgcaaaac ggttgggtac agagctacgg
    13861 ttcccgctgc gtgaagccac cgattgtcat tggtgacatt agccgcccgg caccgattac
    13921 cgtggagtgg gcgaagtatg cgcaatcgct gaccgacaaa ccggtgaaag ggatgctgac
    13981 ggggccggtg accatactct gctggtcgtt cccgcgtgaa gatgtcagcc gtgaaaccat
    14041 cgccaaacag attgcgctgg cgctgcgtga tgaagtggcc gatctggaag ccgctggaat
    14101 tggcatcatc cagattgacg aaccggcgct gcgcgaaggt ttaccgctgc gtcgtagcga
    14161 ctgggatgcg tatctccagt ggggcgtaga ggccttccgt atcaacgccg ccgtggcgaa
    14221 agatgacaca caaatccaca ctcacatgtg ttattgcgag ttcaacgaca tcatggattc
    14281 gattgcggcg ctggacgcag acgtcatcac catcgaaacc tcgcgttccg acatggagtt
    14341 gctggagtcg tttgaagagt ttgattatcc aaatgaaatc ggtcctggcg tctatgacat
    14401 tcactcgcca aacgtaccga gcgtggaatg gattgaagcc ttgctgaaga aagcggcaaa
    14461 acgcattccg gcagagcgcc tgtgggtcaa cccggactgt ggcctgaaaa cgcgcggctg
    14521 gccagaaacc cgcgcggcac tggcgaacat ggtgcaggcg gcgcagaatt tgcgtcgggg
    14581 gtaaatccaa accgggtggt aataccaccc ggtcttttct cattacagcg acttcttccc
    14641 accatactgc ttaaaccatt ccagcatacg ctgccagcca tctttcgcag attcggcatg
    14701 atagctcggg cgataatcag cgttgaatgc atgcccggca tccgggtaca cgataatctc
    14761 tgctttcgca ttagcagccc gcagcgcctg gcgcatcgtt tcaacgctct cctgcggaat
    14821 gctgttatcc tgaccactat ataagccgag aaccggcgcg ttaagatcgg ttgcgatatc
    14881 aacaggttgt ttcggtgaat tcagcgactt gtcgcccgtc agtttgccgt accacgccac
    14941 tgcggctttt agctgtggat tatgcgcggc atacagccag gtgatacgtc caccccagca
    15001 gaatccggtg atcattaaac gatgaacatc gccgccgttg cgggaagccc aactggcgac
    15061 atgatcgaga tcggccagca cctgcgagtc aggcactttt gctaccagac cgctaagcaa
    15121 cgtggggata tcggcaaaat cattcggatc gccttcgcgg aagtaaagtt caggtgcgat
    15181 agccagatac ccctccagcg ccagacggcg acaaatgtcg cggatatgtt catgcacgcc
    15241 aaaaatttcc tgcactacaa tgaccactgg cagtgggcca tcgctttgct ttggtctggc
    15301 atggtaagca ggcatgttat ccccttgtga agggatagag gtgaagcccg ccacaatcgc
    15361 gtcgtccggg gtctgaacga tggtcgaagc gagaggcgat gcagcaggtg caaatccaga
    15421 ttgttgtgtt gttgccatgg tattctccgt acccttataa aaatgttgcg caatgttaac
    15481 tatagtcagc atgcaacaaa tcacattgcc tgaatcggct catcttttat gcagtcctgc
    15541 agaatgaaga gtgatttatg tgatatgaat cacttttggt gggtaaattt atgcaacgca
    15601 tttgcgtcat ggtgatgagt atcacgaaaa aatgttaaac ccttcggtaa agtgtctttt
    15661 tgcttcttct gactaaaccg attcacagag gagttgtata tgtccaagtc tgatgttttt
    15721 catctcggcc tcactaaaaa cgatttacaa ggggctacgc ttgccatcgt ccctggcgac
    15781 ccggatcgtg tggaaaagat cgccgcgctg atggataagc cggttaagct ggcatctcac
    15841 cgcgaattca ctacctggcg tgcagagctg gatggtaaac ctgttatcgt ctgctctacc
    15901 ggtatcggcg gcccgtctac ctctattgct gttgaagagc tggcacagct gggcattcgc
    15961 accttcctgc gtatcggtac aacgggcgct attcagccgc atattaatgt gggtgatgtc
    16021 ctggttacca cggcgtctgt ccgtctggat ggcgcgagcc tgcacttcgc accgctggaa
    16081 ttcccggctg tcgctgattt cgaatgtacg actgcgctgg ttgaagctgc gaaatctatt
    16141 ggcgcgacaa ctcacgttgg cgtgacagct tcttctgata ccttctaccc aggtcaggaa
    16201 cgttacgata cttactctgg tcgcgtagtt cgtcacttta aaggttctat ggaagagtgg
    16261 caggcgatgg gcgtaatgaa ctatgaaatg gaatctgcaa ctctgctgac catgtgtgca
    16321 agtcagggcc tgcgtgccgg tatggtagcg ggtgttatcg ttaaccgcac ccagcaagag
    16381 atcccgaatg ctgagacgat gaaacaaacc gaaagccatg cggtgaaaat cgtggtggaa
    16441 gcggcgcgtc gtctgctgta attctcttct cctgtctgaa ggccgacgcg ttcggccttt
    16501 tgtatttttg cgtagcgcct cgcaggaaat gcctttccaa ctggacgttt gtacagcaca
    16561 attctatttt gtgcgggtaa gttgttgcgt caggaggcgt tgtggatttc tcaatcatgg
    16621 tttacgcagt tattgcgttg gtgggtgtgg caattggctg gctgtttgcc agttatcaac
    16681 atgcgcagca aaaagccgag caattagctg aacgtgaaga gatggtcgcg gagttaagcg
    16741 cggcaaaaca acaaattacc caaagcgagc actggcgtgc agagtgtgaa ttgctcaata
    16801 acgaagtgcg cagcctgcaa agtattaaca cctctctgga ggccgatctg cgtgaagtaa
    16861 ccacgcggat ggaagccgca cagcaacatg ctgacgataa aattcgccag atgattaaca
    16921 gcgagcagcg cctcagtgag cagtttgaaa acctcgccaa ccgtattttt gagcacagca
    16981 atcgccgggt tgatgagcaa aaccgtcaga gtctgaacag cctgttgtcg ccgctacgtg
    17041 aacaactgga cggtttccgc cgtcaggttc aggacagctt cggtaaagaa gcacaagaac
    17101 gccataccct gacccacgaa attcgcaatc tccagcaact caacgcgcaa atggcccagg
    17161 aagcgatcaa cctgacgcgc gcgctgaaag gcgacaataa aacccagggc aactggggcg
    17221 aggtagtatt gacgcgggtg ctggaggctt ccggtctgcg tgaagggtat gaatatgaaa
    17281 cccaggtcag catcgaaaat gacgcccgct cgcggatgca gccggatgtc atcgtgcggc
    17341 tgccgcaggg aaaagatgtg gtgatcgacg ccaaaatgac gctggtcgcc tatgaacgct
    17401 attttaacgc cgaagacgac tacacccgcg aaagcgcgct acaggaacat atcgcgtcgg
    17461 tgcgtaacca tatccgtttg ctgggacgca aagattatca acagctgccg gggctgcgaa
    17521 ctctggatta cgtgctgatg tttattcccg ttgaacccgc ttttttactg gcgcttgacc
    17581 gccagccgga gctgatcacc gaagcgttga aaaacaatat catgctggtt agcccgacta
    17641 cgctgctggt ggcgttgcgt actattgcca acctgtggcg ttatgagcat caaagccgta
    17701 acgcccagca aatcgccgat cgtgccagca agctgtacga caagatgcgt ttgttcatcg
    17761 atgacatgtc cgcgattggt caaagtctcg acaaagcgca ggataactac cgccaggcaa
    17821 tgaaaaaact ctcttcaggt cgcggaaatg tgctggcgca ggcagaagcg tttcgcggtt
    17881 taggagtaga aattaaacgc gagattaatc cggatttggc tgaacaggcg gtgagccagg
    17941 atgaagagta tcgacttcgg tcggttccgg agcagccgaa tgatgaagct tatcaacgcg
    18001 atgatgaata taatcagcag tcgcgctagc ccattagggg tagttaagcc gggtagaaat
    18061 ctagggcatc gacgcccaat ctgttacact tctggaacaa ttttttgatg agcaggcatt
    18121 gagatggtgg ataagtcaca agaaacgacg cactttggtt ttcagaccgt cgcgaaggaa
    18181 caaaaagcgg atatggtcgc ccacgttttc cattccgtgg catcaaaata cgatgtcatg
    18241 aatgatttga tgtcatttgg tattcatcgt ttgtggaagc gattcacgat tgattgcagc
    18301 ggcgtacgcc gtgggcagac cgtgctggat ctggctggtg gcaccggcga cctgacagcg
    18361 aaattctccc gcctggtcgg agaaactggc aaagtggtcc ttgctgatat caatgaatcc
    18421 atgctcaaaa tgggacgcga gaagctgcgt aatatcggtg tgattggcaa cgttgagtat
    18481 gttcaggcga acgctgaggc gctgccgttc ccggataaca cctttgattg catcaccatt
    18541 tcgtttggtc tgcgtaacgt caccgacaaa gataaagcac tgcgttcaat gtatcgcgtg
    18601 ctgaaacccg gcggccgcct gctggtgctt gagttctcga agccaattat cgagccgctg
    18661 agcaaagcct atgatgcata ctccttccat gtgctgccgc gtattggctc actggtcgcg
    18721 aacgacgccg acagctaccg ttatctggca gaatccatcc gtatgcatcc cgatcaggat
    18781 accctgaaag ccatgatgca ggatgccgga ttcgaaagtg tcgactacta caatctgacg
    18841 gcaggggttg tggcgctgca tcgtggttat aagttctgac aggagaccgg aaatgccttt
    18901 taaaccttta gtgacggcag gaattgaaag tctgctcaac accttcctgt atcgctcacc
    18961 cgcgctgaaa acagcccgct cgcgtctgct gggtaaagta ttgcgcgtgg aggtaaaagg
    19021 cttttcgacg tcattgattc tggtgttcag cgaacgccag gttgatgtac tgggcgaatg
    19081 ggcaggcgat gctgactgca ccgttatcgc ctacgccagt gtgttgccga aacttcgcga
    19141 tcgccagcag cttaccgcac tgattcgcag tggtgagctg gaagtgcagg gcgatattca
    19201 ggtggtgcaa aacttcgttg cgctggcaga tctggcagag ttcgaccctg cggaactgct
    19261 ggccccttat accggtgata tcgccgctga aggaatcagc aaagccatgc gcggaggcgc
    19321 aaagttcctg catcacggca ttaagcgcca gcaacgttat gtggcggaag ccattactga
    19381 agagtggcgt atggcacccg gtccgcttga agtggcctgg tttgcggaag agacggctgc
    19441 cgtcgagcgt gctgttgatg ccctgaccaa acggctggaa aaactggagg ctaaatgacg
    19501 ccaggtgaag tacggcgcct atatttcatc attcgcactt ttttaagcta cggacttgat
    19561 gaactgatcc ccaaaatgcg tatcaccctg ccgctacggc tatggcgata ctcattattc
    19621 tggatgccaa atcggcataa agacaaactt ttaggtgagc gactacgact ggccctgcaa
    19681 gaactggggc cggtttggat caagttcggg caaatgttat caacccgccg cgatcttttt
    19741 ccaccgcata ttgccgatca gctggcgtta ttgcaggaca aagttgctcc gtttgatggc
    19801 aagctggcga agcagcagat tgaagctgca atgggcggct tgccggtaga agcgtggttt
    19861 gacgattttg aaatcaagcc gctggcttct gcttctatcg cccaggttca taccgcgcga
    19921 ttgaaatcga atggtaaaga ggtggtgatt aaagtcatcc gcccggatat tttgccggtc
    19981 attaaagcgg atctgaaact tatctaccgt ctggctcgct gggtgccgcg tttgctgccg
    20041 gatggtcgcc gtctgcgccc aaccgaagtg gtgcgcgagt acgaaaagac cttgattgat
    20101 gaactgaatt tgctgcggga atctgccaac gccattcagc ttcggcgcaa ttttgaagac
    20161 agcccgatgc tctacatccc ggaagtttac cctgactatt gtagtgaagg gatgatggtg
    20221 atggagcgca tttacggcat tccggtgtct gatgttgcgg cgctggagaa aaacggcact
    20281 aacatgaaat tgctggcgga acgcggcgtg caggtgttct tcactcaggt ctttcgcgac
    20341 agctttttcc atgccgatat gcaccctggc aacatcttcg taagctatga acacccggaa
    20401 aacccgaaat atatcggcat tgattgcggg attgttggct cgctaaacaa agaagataaa
    20461 cgctatctgg cagaaaactt tatcgccttc tttaatcgcg actatcgcaa agtggcagag
    20521 ctacacgtcg attctggctg ggtgccacca gataccaacg ttgaagagtt cgaatttgcc
    20581 attcgtacgg tctgtgaacc tatctttgag aaaccgctgg ccgaaatttc gtttggacat
    20641 gtactgttaa atctgtttaa tacggcgcgt cgcttcaata tggaagtgca gccgcaactg
    20701 gtgttactcc agaaaaccct gctctatgtc gaaggggtag gacgccagct ttatccgcag
    20761 ctcgatttat ggaaaacagc gaagcctttc ctggagtcgt ggattaaaga tcaggtcggt
    20821 attcctgccc tggtgagagc atttaaagaa aaagcgccgt tctgggtcga aaaaatgcca
    20881 gaactgcctg aactggttta cgacagtttg cgccagggca agtatttaca gcacagtgtt
    20941 gataagattg cccgcgagct tcagtcaaat catgtacgtc agggacaatc gcgttatttt
    21001 ctcggaattg gcgctacgtt agtattaagt ggcacattct tgttggtcag ccgacctgag
    21061 tgggggctga tgcccggctg gttaatggca ggtggtctga tcgcctggtt tgtcggttgg
    21121 cgcaaaacac gctgattttt tcatcgctca aggcgggccg tgtaacgtat aatgcggctt
    21181 tgtttaatca tcatctacca cagaggaaca tgtatgggtg gtatcagtat ttggcagtta
    21241 ttgattattg ccgtcatcgt tgtactgctt tttggcacca aaaagctcgg ctccatcggt
    21301 tccgatcttg gtgcgtcgat caaaggcttt aaaaaagcaa tgagcgatga tgaaccaaag
    21361 caggataaaa ccagtcagga tgctgatttt actgcgaaaa ctatcgccga taagcaggcg
    21421 gatacgaatc aggaacaggc taaaacagaa gacgcgaagc gccacgataa agagcaggtg
    21481 taatccgtgt ttgatatcgg ttttagcgaa ctgctattgg tgttcatcat cggcctcgtc
    21541 gttctggggc cgcaacgact gcctgtggcg gtaaaaacgg tagcgggctg gattcgcgcg
    21601 ttgcgttcac tggcgacaac ggtgcagaac gaactgaccc aggagtttca ggacagtctg
    21661 aaaaaggttg aaaaggcgag cctcactaac ctgacgcccg aactgaaagc gtcgatggat
    21721 gaactacgcc aggccgcgga gtcgatgaag cgttcctacg ttgcaaacga tcctgaaaag
    21781 gcgagcgatg aagcgcacac catccataac ccggtggtga aagataatga agctgcgcat
    21841 gagggcgtaa cgcctgccgc tgcacaaacg caggccagtt cgccggaaca gaagccagaa
    21901 accacgccag agccggtggt aaaacctgct gcggacgctg aaccgaaaac cgctgcccct
    21961 tccccttcgt cgagtgataa accgtaaaca tgtctgtaga agatactcaa ccgcttatca
    22021 cgcatctgat tgagctgcgt aagcgtctgc tgaactgcat tatcgcggtg atcgtgatat
    22081 tcctgtgtct ggtctatttc gccaatgaca tctatcacct ggtatccgcg ccattgatca
    22141 agcagttgcc gcaaggttca acgatgatcg ccaccgacgt ggcctcgccg ttctttacgc
    22201 cgatcaagct gacctttatg gtgtcgctga ttctgtcagc gccggtgatt ctctatcagg
    22261 tgtgggcatt tatcgcccca gcgctgtata agcatgaacg tcgcctggtg gtgccgctgc
    22321 tggtttccag ctctctgctg ttttatatcg gcatggcatt cgcctacttt gtggtctttc
    22381 cgctggcatt tggcttcctt gccaataccg cgccggaagg ggtgcaggta tccaccgaca
    22441 tcgccagcta tttaagcttc gttatggcgc tgtttatggc gtttggtgtc tcctttgaag
    22501 tgccggtggc aattgtgctg ctgtgctgga tggggattac ctcgccagaa gacttacgca
    22561 aaaaacgccc gtatgtgctg gttggtgcat tcgttgtcgg gatgttgctg acgccgccgg
    22621 atgtcttctc gcaaacgcta ttggcgatcc cgatgtactg tctgtttgaa atcggtgtct
    22681 tcttctcacg cttttacgtt ggtaaagggc gaaatcggga agaggaaaac gacgctgaag
    22741 cagaaagcga aaaaactgaa gaataaattc aaccgcccgt cagggcggtt gtcatatgga
    22801 gtacaggatg tttgatatcg gcgttaattt gaccagttcg caatttgcga aagaccgtga
    22861 tgatgttgta gcgcgcgctt ttgacgcggg agttaatggg ctactcatca ccggcactaa
    22921 cctgcgtgaa agccagcagg cgcaaaagct ggcgcgtcag tattcgtcct gttggtcaac
    22981 ggcgggcgta catcctcacg acagcagcca gtggcaagct gcgactgaag aagcgattat
    23041 tgagctggcc gcgcagccag aagtggtggc gattggtgaa tgtggtctcg actttaaccg
    23101 caacttttcg acgccggaag agcaggaacg cgcttttgtt gctcagctac gcattgccgc
    23161 agaattaaac atgccggtat ttatgcactg tcgcgatgcc cacgagcggt ttatgacatt
    23221 gctggagccg tggctggata aactgcctgg tgcggttctt cattgcttta ccggcacacg
    23281 cgaagagatg caggcgtgcg tggcgcgtgg aatttatatc ggcattaccg gttgggtttg
    23341 cgatgaacga cgcgggctgg agctgcggga attgttgccg ttgattccgg cggagaaatt
    23401 gctgatcgaa actgatgcgc cgtatctgct cccgcgcgat ctcacgccaa agccatcatc
    23461 ccggcgcaac gagccagccc atctgcccca tattttgcaa cgtattgcgc actggcgtgg
    23521 agaagatgcc gcatggctgg ctgccaccac ggatgccaat gtcaaaacac tgtttgggat
    23581 tgcgttttag agtttgcgga actcggtgtt cttcacactg tgcttaatct ctttattaat
    23641 aagattaagc aatagcatgg agcgagcctc accatcgggt tcggtgaaaa tggcctgaaa
    23701 gccttcgaac gcgccttcgg taataatcac cttatcaccc ggataagggg gtgccggatc
    23761 gacaatgtct ttcggtttat ataccgatag ctgatgaatc accgccgatg ggactatcgc
    23821 tggcgacgcg ccaaagcgca caaagtggct gacgccacgc gtagcgctga tggtcgtggt
    23881 atgaatcact tctggatcaa attccacaaa caggtagtta gggaataacg gttcactgac
    23941 tgcggtacgt ttaccccgta cgattttttc cagggtgatc atcggagcga ggcaattaac
    24001 cgcctgtctc tcaaggtgtt cctgggcacg ttgaagttgc ccgcgcttgc agtacagtaa
    24061 ataccaggat tgcataatga ctcttatccg tttaatcggg gcgcaaggat agcaaaagct
    24121 ttacgctaag ttaattatat tccccggttt gcgttatacc gtcagagttc acgctaattt
    24181 aacaaattta cagcattgca aagatgaacg ccgtataatg ggcgcagatt aagaggctac
    24241 aatggacgcc atgaaatata acgatttacg cgacttcctg acgctgcttg aacagcaggg
    24301 tgagctaaaa cgtatcacgc tcccggtgga tccgcatctg gaaatcactg aaattgctga
    24361 ccgcactctg cgtgctggtg ggcctgcgct gttgttcgaa aaccctaaag gctactcaat
    24421 gccggtgctg tgcaacctgt tcggtacgcc aaagcgcgtg gcgatgggca tggggcagga
    24481 agatgtttcg gcgctgcgtg aagttggtaa attattggcg tttctgaaag agccggagcc
    24541 gccaaaaggt ttccgcgacc tgtttgataa actgccgcag tttaagcagg tgttaaacat
    24601 gccgacaaag cgactgcgtg gtgcgccctg ccaacaaaaa atcgtctctg gcgatgacgt
    24661 cgatctcaat cgcattccca ttatgacctg ctggccggaa gatgccgcgc cgctgattac
    24721 ctgggggctg accgtaacgc gcggcccgca taaagagcgg cagaatctgg gcatttatcg
    24781 ccagcagctg attggtaaaa acaaactgat tatgcgctgg ctgtcgcatc gcggcggcgc
    24841 gctggattat caggagtggt gtgcggcgca tccgggcgaa cgtttcccgg tttctgtggc
    24901 gctgggtgcc gatcctgcca cgattctcgg tgcagtcacc cccgttccgg atacgctttc
    24961 agagtatgcg tttgccggat tgctacgcgg caccaaaacc gaagtagtaa agtgtatttc
    25021 caatgacctc gaagtgccag ccagtgccga aatcgtcctg gaagggtata tcgatcctgg
    25081 tgagatggcg ccggaagggc cgtatggtga ccacacaggt tactataatg aagtcgatag
    25141 tttcccggtg tttaccgtga cgcatattac ccagcgtgaa gatgcgattt accattccac
    25201 ctataccggg cgtccgccag atgagcccgc ggtactgggc gtggcgttga acgaagtgtt
    25261 tgtaccgatt ctgcaaaaac agttcccgga aattgtcgat ttttacctgc cgccggaagg
    25321 ctgttcttat cgtctggcgg tagtgacgat caaaaaacag tacgccggac acgcgaagcg
    25381 cgtcatgatg ggcgtctggt cgttcttacg ccagtttatg tacactaaat ttgtgatcgt
    25441 ttgcgatgat gacgtcaacg cacgcgactg gaacgatgtg atttgggcga ttaccacccg
    25501 tatggacccg gcgcgggata ctgttctggt agaaaatacg cctattgatt atctggattt
    25561 tgcctcgcct gtctccgggc tgggttcaaa aatggggctg gatgccacga ataaatggcc
    25621 gggggaaacc cagcgtgaat ggggacgtcc catcaaaaaa gatccagatg ttgtcgcgca
    25681 tattgacgcc atctgggatg aactggctat ttttaacaac ggtaaaagcg cctgatgcgc
    25741 gtttgttttg ccctatttat cgatccgaca gagaaagcgc atgacaacct taagctgtaa
    25801 agtgacctcg gtagaagcta tcacggatac cgtatatcgt gtccgcatcg tgccagacgc
    25861 ggccttttct tttcgtgctg gtcagtattt gatggtagtg atggatgagc gcgacaaacg
    25921 tccgttctca atggcctcga cgccggatga aaaagggttt atcgagctgc atattggcgc
    25981 ttctgaaatc aacctttacg cgaaagcagt catggaccgc atcctcaaag atcatcaaat
    26041 cgtggtcgac attccccacg gagaagcgtg gctgcgcgat gatgaagagc gtccgatgat
    26101 tttgattgcg ggcggcaccg ggttctctta tgcccgctcg attttgctga cagcgttggc
    26161 gcgtaaccca aaccgtgata tcaccattta ctggggcggg cgtgaagagc agcatctgta
    26221 tgatctctgc gagcttgagg cgctttcgtt aaagcaccct ggtctgcaag tggtgccggt
    26281 ggttgaacaa ccggaagcag actggcgtgg gcgtactggt accgtgttaa cggcggtatt
    26341 gcaggatcat ggtacgctgg cagagcatga tatctatatt gccggacgtt ttgagatggc
    26401 gaaaattgcc cgtgacctgt tttgcagtga gcgtaatgcg cgggaagatc gcctgtttgg
    26461 cgatgcgttt gcatttatct gagatataaa aaaacccgcc cctgacaggc gggaagaacg
    26521 gcaactaaaa ctttattcag tggcatttag atctatgtcg catccggcaa ttggtgcacg
    26581 atgcctgatg cgacgctggc gcgtcttatc aggcctacat tggtgccgga tcggtagacc
    26641 ggataaggcg ttcacgccgc atccggcaag tggttaaacc cgctcaaaca ccgtcgcaat
    26701 accctgaccc ataccgatac acatcgtcgc cagaccaaac tgaacgtctt tgcgttccat
    26761 cagattcagc agcgtggtgc tgatacgcgc accggaacaa cccagcggat gacccagcgc
    26821 gatcgcacca ccattgaggt tgatcttctc gtcaatctgc tccattagcc cgagatcttt
    26881 aatgcacggc aggatctgcg cggcaaaggc ttcgttcatc tcaaacacgc cgatatcgct
    26941 ggcagaaagc cccgcttttt tcagcgccag cttcgaggcc ggaaccgggc cgtaacccat
    27001 aatcgatggg tcacaaccaa cgaccgccat cgaacgcaca cgagcgcgcg gcttaagacc
    27061 taattcatgg gcgcggcttt cactcatcac cagcatggca gctgcgccat cggaaagtgc
    27121 agaagatgtg cccgccgtta ccgtaccgtt tactggatca aacgccggac gcagcgtggc
    27181 gagggcttcc acggtggttt ccgggcgaat cacttcgtcg taattaaact gcttcaggac
    27241 gccgtcggca tcgtgaccac cagttgggat gatttcattt ttaaatgcgg ccgactgcgt
    27301 ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca tcctgcattt cacggctgat
    27361 accgtgcata cgcgccagca tttctgccgt taagcccatc atgcccgccg ctttggcgac
    27421 attgcggctc aggccggggt gaaaatcgac gccgtgactc atcggcacat ggcccatatg
    27481 ctccacgccg ccaaccagac atgcctgcgc atcgccagtc atgatcattc gtgctgcgtc
    27541 atgcagtgcc tgcatggatg aaccacacaa gcgattgacg gtaaccgccg ggacagagtg
    27601 cggcacttct gccagcagcg ccgcgttacg ggcgatgtta aaaccctgct ccagcgtctg
    27661 ttgcacacaa ctccagtaaa tatcgtcgag ggccgccgct tccagcgccg ggttacgcgc
    27721 cagcaggcta cgcattaaat gagcggagag atcttccgca cgcacgttac gaaaagcacc
    27781 gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca atgacaacct gttccattgt
    27841 gactccttaa gccgttttca ggtcgccaac cggacgggct ggctcaaccg gaggatagta
    27901 cggttcgtta tgacgcgctt tattacgcag accttccggc acttcataca gcgggccgag
    27961 gtgctgatat tgctgtgcca tatcgaggta ttttgcgcta ccgagggtgt ccagccagcg
    28021 gaacgcgccg ccgtggaacg gagggaagcc caggccgtag accagcgcca tatccgcttc
    28081 cgccggagtg gcgataatgc cctcttccag acagcgcacc acttcgttga ccatcgggat
    28141 catcatgcgg gcgataatct cttcttcgct gaaatcgcgc ttcggttggc tcacttctgc
    28201 cagcaggtct tcaacggcgg cgtcttcttc tttcttcggc ttacctttgc tgtcttcttt
    28261 ataacgccag aaaccgaggc cgttcttctg accaaagcgg ttggcatcaa acagcgcgtc
    28321 gatggcatcg cggtaatctt tctgcatccg ctgcgggaag cctgctgcca tgacagcctg
    28381 agcgtgatgc gcggtatcaa tgcccacaac gtccagcaga tatgccgggc ccatcggcca
    28441 gccaaactgt ttttccatca ctttgtcgat cttgcggaaa tccgcgccgt cgcgcagcag
    28501 ctggctgaaa ccggcgaaat acgggaacag cacgcggtta acaaagaagc cggggcagtc
    28561 gttaaccaca atcggcgtct tgcccatctt gctcgcccag gcgacaactt tcgcgatggt
    28621 ttcgtcggag cttttctcgc cgcgaataat ttctaccaac ggcattcggt ggaccgggtt
    28681 aaagaagtgc atcccgcaga agttttccgg gcgttccagc gcgttggcca gttcgctgat
    28741 aggaatggtt gaagtgttag acgccagcac ggtatcctgg cgtacttttt gttcggtttc
    28801 tgccagtacg gcttttttca ctttcgggtt ttcaacaacc gcttctacca caatatccac
    28861 gcggtcaaat ccggcgtagt cgagcgttgg gtggatcgtg gagatcacgc cagccagttt
    28921 cagaccatcg atcttgccgc gctcaagctg cttgttcagc agtttcgcgg cttcggtcat
    28981 gccgagggtt aacgacttgt cgttgatatc tttcatgaca accggcacgc ctttccacgc
    29041 ggactggtaa gcgataccgc cacccatgat gcctgcgccc agcacagcag cctgtttcgg
    29101 ggtttcaacg tctttggtga gtttcttcgc tttgcctttt acatattgat cgttaaggaa
    29161 aatgccgacc agtgcgcgag cttcgttggt atgcgccagc gggacaaaac ttttgttttc
    29221 cagatttaag gcttcttcac gaccaaaacg ggccgcagct tcaatggttt ttactgcggt
    29281 gatgggggcc ggataatgtt tccccgccgt ttgcgcgacc attcctttag cgatggtgaa
    29341 gctcatggtg gcttcaatct tgctcagttt tagcggttcc agcttcggct gacgtttcgc
    29401 tttccagtcg aggtcgccgt taatggcctg gcgtaaaatc gccatcgccc cttcaatcag
    29461 tttttctgct ttgactacgc catccaccag accgattttc agcgcctgat ccgcgccgac
    29521 atctttaccg gcggcaatga tttccagtgc actgtcagcg cccagcatac gtggcatacg
    29581 tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt tccggcagac cgatgcgcag
    29641 atccggcgtc gccagacgat aatcggtcgc cagcacgcat tcgcagccac cacccagcgc
    29701 atagccgttg acggcagcaa tggtcggcac cggcagatct tccaggcgat taaacacgct
    29761 attggcaaag tgcagccact gacttaactg ttcttcagga acgaggaaca gggacaaaaa
    29821 ttcggtgata tcagcaccga cgataaaggc tgctttgttc gaacgcagca gcagcccttt
    29881 tagatctgat tgctgttcca gcacgccgat ggcctcgccg aggctggcga cggtcgcagt
    29941 gtcgagttta ttaactgaac ctggggcatc aaataccagt tcggcaatgc catcttccag
    30001 ccagtcaagg tacagggtgt cgcctttgta aagcatgtca gtctcctgaa tccgcaaggt
    30061 gatctggtcg taccagatgc atcggagtgt gtgttttgtg ttaaaaatat gcaaataaaa
    30121 gattaaagaa atgccgatct gatcacgctc ggcagaaatc acgctctgga tgaacgatgt
    30181 gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt aaaaaatgga atcactggcc
    30241 tcgctctata aaaatcatat agctacctta caagaacgga ctcgcgatgc gctggcgcgc
    30301 ttcaagctgg atgcattact tattcactcc ggcgagctgt tcaacgtttt tctcgacgat
    30361 catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt gggtgccggt aactcaggtg
    30421 ccaaactgct ggttgctggt ggatggcgtg aacaagccga aactgtggtt ctatctgccg
    30481 gttgattact ggcacaacgt cgaaccgctg ccgacctcct tctggactga agatgtggaa
    30541 gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc tgcctgctgc gcgcggcaat
    30601 atcggttata tcggtccggt gccggaacgt gcgctgcaac tgggtattga ggccagcaac
    30661 atcaacccga aaggggtgat cgactacctg cattactacc gctccttcaa aaccgagtac
    30721 gaactggcct gtatgcgtga agcgcagaaa atggcggtca atggtcatcg tgcggcagaa
    30781 gaagcgttcc gttctggcat gagcgagttc gatatcaata ttgcctatct gaccgcgacc
    30841 ggtcatcgtg ataccgacgt accttacagc aacattgtgg cgcttaacga acacgctgcg
    30901 gtgttgcatt acaccaaact ggaccaccag gcaccggaag agatgcgcag cttcctgctg
    30961 gatgccgggg ccgaatataa cggctatgcg gctgacctga cccgtacctg gtcggcaaaa
    31021 agcgacaacg actacgcaca gctggtgaaa gacgtaaatg atgaacaact ggcgctgatc
    31081 gccaccatga aagcaggcgt cagctatgtg gattaccaca tccagttcca tcagcgcatc
    31141 gccaaactgc tgcgtaaaca tcaaatcatc accgatatga gtgaagaagc gatggtcgaa
    31201 aacgatctca ccggaccgtt tatgccgcac ggtattggcc atccgctggg cctgcaggtg
    31261 catgacgtcg ccggttttat gcaggatgat agcggtacac acctcgcggc accggcaaaa
    31321 tatccgtacc tgcgctgcac ccgtattctc cagccgggca tggtgttaac catcgaaccg
    31381 ggtatctact tcattgaatc gcttctggca ccgtggcgtg aagggcagtt cagcaagcac
    31441 ttcaactggc agaaaattga agcactgaaa ccgttcggcg gcattcgtat cgaagacaac
    31501 gtggtgatcc acgaaaacaa cgtggaaaac atgacccggg atctgaaact ggcgtgatgg
    31561 aaagctggtt aattcctgcg gcaccggtca cggtcgttga agagatcaaa aagagccgct
    31621 tcataacgat gttggcgcat accgatggcg ttgaggcggc gaaagcgttt gttgaatcgg
    31681 tgcgggcaga acaccccgat gcccgccacc attgcgtggc gtgggtggcg ggtgcgccgg
    31741 atgattctca acagctgggt ttctctgacg acggggagcc ggcgggaacg gcaggtaaac
    31801 cgatgctcgc ccagctaatg ggcagcggcg tcggggaaat taccgctgtg gtagtgcgct
    31861 actacggcgg catattgctt ggcaccggtg ggttagtgaa agcgtatggc ggcggcgtga
    31921 atcaggcgct gcgccagcta acgacccaac gcaagacgcc attaaccgaa tatactttgc
    31981 aatgtgaata tagtcagtta accggcattg aagcgttgct ggggcagtgt gacggcaaaa
    32041 ttatcaacag tgattatcag gcattcgttc tgctgcgggt ggcgcttccg gcggcgaaag
    32101 tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg ttcattgcaa ttgttagcga
    32161 ttgaagaata atccccactt cgttttgcag aactaaggaa gcggcagaga tgcattttcg
    32221 cgccattacc cgaatcgttg gactactggt catcttattt tcagggacca tgattatccc
    32281 tgggctggta gcactcatct accgggatgg agcgggccgc gcttttaccc agaccttttt
    32341 tgtcgccctc gccattggct ctatgctgtg gtggccgaac cgcaaagaga agggcgaact
    32401 gaaatcccgt gaggggtttc tgatagtggt gctgttttgg accgtgctgg gtagtgtcgg
    32461 tgcgctccct tttatcttct cggaaagccc gaacctcacg attaccgatg cgttttttga
    32521 atctttctct ggcctgacca ccaccggggc cactacgctg gtggggctgg attcgctccc
    32581 tcatgccatc ctcttttatc gccagatgct gcaatggttt ggcgggatgg ggatcatcgt
    32641 gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg atgcagctct atcgcgcaga
    32701 aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga attgcggaaa cggcgaaaac
    32761 cctgtggttg atttatgtct tgctgaccgt cgcctgtgcg ctggcgttgt ggtttgccgg
    32821 aatggatgcc attgatgcca tcggccatag ctttgcgact atcgctattg gcggcttctc
    32881 gacacatgat gccagtatcg gttatttcga cagtccgact attaacacta tcattgctat
    32941 cttcctgctg atctccggct gtaactacgg tctgcacttt tcactgttaa gtgggcgtag
    33001 tctgaaggtt tattggcgcg atccggaatt tcgcatgttt atcggcgtac agtttacgct
    33061 ggtggttatt tgtacactcg tactgtggtt tcataatgtc tacagttcgg cgctgatgac
    33121 aattaaccag gcgtttttcc aggtggtatc gatggcgaca accgccgggt ttacgactga
    33181 cagcattgcc cgctggccgc tctttttgcc ggtactgctt ttatgttcag cttttatcgg
    33241 cggttgcgcc gggtcaacgg gcggtggcct gaaagtgatc cgcatcctgc tgctgtttaa
    33301 gcaggggaac cgtgagctga aacgactggt gcatccgaac gccgtctata gcattaagct
    33361 ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt tggggatttt tctccgccta
    33421 tgcattggtg tttattgtca gtatgctggc gattatcgcc acgggcgtgg atgatttttc
    33481 tgcctttgcc tcagttgttg cgacattgaa taacctgggg ccagggcttg gcgtggttgc
    33541 tgataacttt accagtatga acccggtggc taaatggatc ctgattgcca acatgctgtt
    33601 tggtcgtctc gaggtcttta cattgctggt gctctttacc ccgactttct ggcgtgaatg
    33661 atggagtaat acgtgaaaac attaattctt ttctcaacaa gggacggaca aacgcgcgag
    33721 attgcctcct acctggcttc ggaactgaaa gaactgggga tccagacgga tgtcgccaat
    33781 gtgcaccgca ttgaagaacc acaatgggaa aactatgacc gtgtggtcat tggtgcttct
    33841 attcgctatg gtcactacca ctctgcgttc caggaatttg tcaaaaaaca tgcgacgcga
    33901 ctgaattcga tgccgagcgc cttttattcc gtaaacctgg tggcgcgcaa accggagaag
    33961 cgtactccac agaccaacag ctacgcgcgg aagtttctga tgaactcgca atggcgtccc
    34021 gatcgctgcg cggtcattgc cggggcgctg cgttacccac gttatcgctg gtacgaccgt
    34081 tttatgctca agctgattat gaagatgtca ggcggtgaaa cggatacgcg caaagaagtt
    34141 gtctataccg attgggagca ggtggcgaat ttcgcccgag aaatcgccca tttaaccgac
    34201 aaaccgacgc tgaaataagc ataaagaata aaaaatgcgc ggtcagaaaa ttattttaaa
    34261 tttcctcttg tcaggccgga ataactccct ataatgcgcc accactgaca cggaacaacg
    34321 gcaaacacgc cgacgggtca gcggggttct cctgagaatt ccg
//
LOCUS       DADLSA010000030        34180 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.030, whole genome shotgun sequence.
ACCESSION   DADLSA010000030 DADLSA010000000
VERSION     DADLSA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.030"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(78..479)
                     /gene="nikR"
                     /locus_tag="JN691_001434"
     CDS             complement(78..479)
                     /gene="nikR"
                     /locus_tag="JN691_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAY7242912.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(485..1291)
                     /gene="nikE"
                     /locus_tag="JN691_001435"
     CDS             complement(485..1291)
                     /gene="nikE"
                     /locus_tag="JN691_001435"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAY7242913.1"
                     /translation="MSLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            complement(1288..2052)
                     /gene="nikD"
                     /locus_tag="JN691_001436"
     CDS             complement(1288..2052)
                     /gene="nikD"
                     /locus_tag="JN691_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAY7242914.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2052..2885)
                     /gene="nikC"
                     /locus_tag="JN691_001437"
     CDS             complement(2052..2885)
                     /gene="nikC"
                     /locus_tag="JN691_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAY7242915.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRMSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYSRMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(2882..3826)
                     /gene="nikB"
                     /locus_tag="JN691_001438"
     CDS             complement(2882..3826)
                     /gene="nikB"
                     /locus_tag="JN691_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAY7242916.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(3826..5400)
                     /gene="nikA"
                     /locus_tag="JN691_001439"
     CDS             complement(3826..5400)
                     /gene="nikA"
                     /locus_tag="JN691_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAY7242917.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADN
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5511..6098)
                     /gene="acpT"
                     /locus_tag="JN691_001440"
     CDS             complement(5511..6098)
                     /gene="acpT"
                     /locus_tag="JN691_001440"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAY7242918.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6153..7202)
                     /locus_tag="JN691_001441"
     CDS             complement(6153..7202)
                     /locus_tag="JN691_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7242919.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RNLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7301..8551
                     /locus_tag="JN691_001442"
     CDS             7301..8551
                     /locus_tag="JN691_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7242920.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPEHVPGAASSS"
     gene            complement(8555..9112)
                     /gene="dcrB"
                     /locus_tag="JN691_001443"
     CDS             complement(8555..9112)
                     /gene="dcrB"
                     /locus_tag="JN691_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAY7242921.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9185..9850)
                     /locus_tag="JN691_001444"
     CDS             complement(9185..9850)
                     /locus_tag="JN691_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAY7242922.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQRFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10071..10316
                     /gene="tusA"
                     /locus_tag="JN691_001445"
     CDS             10071..10316
                     /gene="tusA"
                     /locus_tag="JN691_001445"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAY7242923.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10418..12616)
                     /gene="zntA"
                     /locus_tag="JN691_001446"
     CDS             complement(10418..12616)
                     /gene="zntA"
                     /locus_tag="JN691_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAY7242924.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTVNALRLLRRR"
     gene            complement(12690..13316)
                     /locus_tag="JN691_001447"
     CDS             complement(12690..13316)
                     /locus_tag="JN691_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAY7242925.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            13457..13816
                     /locus_tag="JN691_001448"
     CDS             13457..13816
                     /locus_tag="JN691_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAY7242926.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(13819..14088)
                     /locus_tag="JN691_001449"
     CDS             complement(13819..14088)
                     /locus_tag="JN691_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAY7242927.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14078..14674)
                     /gene="rsmD"
                     /locus_tag="JN691_001450"
     CDS             complement(14078..14674)
                     /gene="rsmD"
                     /locus_tag="JN691_001450"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAY7242928.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            14824..16320
                     /gene="ftsY"
                     /locus_tag="JN691_001451"
     CDS             14824..16320
                     /gene="ftsY"
                     /locus_tag="JN691_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAY7242929.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16323..16991
                     /gene="ftsE"
                     /locus_tag="JN691_001452"
     CDS             16323..16991
                     /gene="ftsE"
                     /locus_tag="JN691_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAY7242930.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            16984..18042
                     /gene="ftsX"
                     /locus_tag="JN691_001453"
     CDS             16984..18042
                     /gene="ftsX"
                     /locus_tag="JN691_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAY7242931.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18287..19141
                     /gene="rpoH"
                     /locus_tag="JN691_001454"
     CDS             18287..19141
                     /gene="rpoH"
                     /locus_tag="JN691_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAY7242932.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19412..20501
                     /locus_tag="JN691_001455"
                     /pseudo
     CDS             19412..20501
                     /locus_tag="JN691_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
     gene            complement(20700..21083)
                     /gene="panM"
                     /locus_tag="JN691_001456"
     CDS             complement(20700..21083)
                     /gene="panM"
                     /locus_tag="JN691_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAY7242933.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTALQGGWEKR"
     gene            21507..22616
                     /gene="livk"
                     /locus_tag="JN691_001457"
     CDS             21507..22616
                     /gene="livk"
                     /locus_tag="JN691_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAY7242934.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLLNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            22664..23590
                     /gene="livH"
                     /locus_tag="JN691_001458"
     CDS             22664..23590
                     /gene="livH"
                     /locus_tag="JN691_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAY7242935.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            23587..24864
                     /locus_tag="JN691_001459"
     CDS             23587..24864
                     /locus_tag="JN691_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAY7242936.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGRDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            24861..25628
                     /gene="livG"
                     /locus_tag="JN691_001460"
     CDS             24861..25628
                     /gene="livG"
                     /locus_tag="JN691_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAY7242937.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            25630..26343
                     /gene="livF"
                     /locus_tag="JN691_001461"
     CDS             25630..26343
                     /gene="livF"
                     /locus_tag="JN691_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAY7242938.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            26740..28056
                     /gene="ugpB"
                     /locus_tag="JN691_001462"
     CDS             26740..28056
                     /gene="ugpB"
                     /locus_tag="JN691_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HAY7242939.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNLGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            28154..29041
                     /gene="ugpA"
                     /locus_tag="JN691_001463"
     CDS             28154..29041
                     /gene="ugpA"
                     /locus_tag="JN691_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HAY7242940.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            29038..29883
                     /gene="ugpE"
                     /locus_tag="JN691_001464"
     CDS             29038..29883
                     /gene="ugpE"
                     /locus_tag="JN691_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAY7242941.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            29963..30850
                     /gene="ugpC"
                     /locus_tag="JN691_001465"
     CDS             29963..30850
                     /gene="ugpC"
                     /locus_tag="JN691_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAY7242942.1"
                     /translation="MADGEFIVMVGPPGCGKSTLLRMVAGLERVTEGDIWINDQRVTE
                     MEPKDRGIAMVFQNYALYPHMNVEENMAWGLKIRGMGKQQIAERVKEAARILELDGLL
                     KRRPRELSGGQRQRVAMGRAIVRDPAVFLFDEPLSNLDAKLRVQMRLELQQLHRRLKT
                     TSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGTPVEVYEKPASLFVASFIGSPAMNLLT
                     GRMNNEGTHFELDGGIVLPLNGGYRQYAGRKMTLGIRPEHIALSSQAEAGGATGASGA
                     PDGRQHAVAASGGKSAASF"
     gene            30872..31615
                     /gene="ugpQ"
                     /locus_tag="JN691_001466"
     CDS             30872..31615
                     /gene="ugpQ"
                     /locus_tag="JN691_001466"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY7242943.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(31602..32042)
                     /locus_tag="JN691_001467"
     CDS             complement(31602..32042)
                     /locus_tag="JN691_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAY7242944.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            32162..33904
                     /gene="ggt"
                     /locus_tag="JN691_001468"
     CDS             32162..33904
                     /gene="ggt"
                     /locus_tag="JN691_001468"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAY7242945.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAAPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(33963..>34180)
                     /locus_tag="JN691_001469"
     CDS             complement(33963..>34180)
                     /locus_tag="JN691_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7242946.1"
                     /translation="NRGVASALMREMIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFG
                     FEIEGTGKKYALRNGEYVDAYYMARVK"
BASE COUNT         7920 a         8914 c         9545 g         7801 t
ORIGIN      
        1 atctatctat gcaactgaac ttaattttat aaattaagtt cagttaatat cttttctttc
       61 cttgttttag aaataactca atcttccttc ggcaggcact gcaaatgccc gtgccgcacg
      121 ccgcgctggg cgataacgtc atcggcaaaa tgctgcacgt cacccatgtc acctttcaac
      181 acggcgattt ccagacagtc gtcgtggttg atgtgcacat gtagcgtggc gacggagagg
      241 tcgtggtgat gatgctgggt ggaaacaata cggctggcta agtcgcgttt ttcgtgttca
      301 tacacatacg acagcaccgc gaaaccttgt gtgccgtgct gctgggtagc ttcctgcgcc
      361 agggcactac gcagaatatc gcggatagct tcggaacggt tgttataacc acgacgctgg
      421 ctcaggctgt ccagcgtctc cagtaaatcg tcatcaagcg tgatggtgac tcgttgcatt
      481 ggcgttaaac cttttctgag gtgcgacggc gcacggggaa tgcgggtaat accgcgtttt
      541 gtagcacacg tccggcgtca gaggaaaagg ttaatttatc tcccaccgcc tgggtttcga
      601 cgatttgtcc gttgtccatc accatcactc gctggcaaaa gcgttccacc aggcgtaagt
      661 catgagtgat gaacaggcag gcagtgccaa actgttgttg tagctttttc agcaggcgaa
      721 tgacgcccgc ctgtaacacg agatcaaggt tagaaacggc ttcatccaga atcagcaatt
      781 tcggttcgac cgccagcgcg cgtgccaggc agacgagctg gagctggccg ccgcttaact
      841 gcggtgggcg tttgtcgaga acgctgtcat cgagatcgac cgccttcagc atttcgctgg
      901 cgcgcgccag ttgttcggat tttttcagtg atagcaggtg gcgcatcggt tcacgcagga
      961 tctcgcgcac ggttttgcgc ggattcacgg cactgatgga gtcctgaaac accatctgaa
     1021 tatcacggcg gaaggcttta cgttgggcgc gattaagttt cgccagcgat tcgccgcgcc
     1081 agctaatatt cccttgtgag ggagactcta agcctaccag cagccgcgcg agggtgcttt
     1141 ttccgcagcc gctgcgtccc agcagggcga cggtttcgcc gcttttcagg gtcagggaaa
     1201 cgttattcag caccgcctga tgttgatgtt ttccgctaaa tccaccgtgg gcatagtgat
     1261 gggaaaggcc gcagacgtta agtaagctca tgatgccagc tccataccgt agagggcgag
     1321 atgagcggaa accaggctgc gcgtcaccgt atgtttgggg gcgttaaaca gcgtttctac
     1381 atcgccctgt tcgacaattt taccttgtga catcaccgcc acgtcatccg ccagacgcgc
     1441 cacaacgccc atatcatggg tgaccagcag cattcccggc gcttgttttt gcataatgct
     1501 ttccagcaga tcgaggatgc gcgcctgtgc taccacgtcg aggtcggtgg tcggttcatc
     1561 ggcgatgata aacggtgatt cacacagcac cgccatcgca atcatcatgc gctgcaacat
     1621 gccgccgctc atctcgaacg ggtacagctt cagcacgcgc gcggcgtttt ccagccccac
     1681 cgcttctatg gcagcggtaa gcgtagcgtc atcggcgggt ttccctaacg ccaggcaggt
     1741 ttcccgcgcg tgggtgtgca tggtgtgcag tggattaaag gcgctgcgcg ggttctgcat
     1801 gatggtggca attttgatgc cgcgcagggc gcaaggcgaa accggtttgc catcggctaa
     1861 aatttccccc gccgtctggc gaacgccagc gggcaaaatg cccagcgtcg cggcgcaggt
     1921 taatgatttc ccgctgccgc taccgccgac taacgccagc acgcgcccgc gttgcagggt
     1981 taacgataca ccgtgtacca gcggctgcgc ggcctgtagc gcgatattac gtagttcaat
     2041 ctgttgcggc attagtgtgc gtgctccgtc accagatgag gatccagatg atcgcgcagt
     2101 gcgtcaccca ccaggttaaa ggccatcacg ctgataaaca gcgccagccc cggccagaac
     2161 atttgcagcg gctgggtcca gatatactgg cgcgcgtcgt taatcatcac gccccattcg
     2221 gcggtcggcg cggtcacacc gaggccaagg aaagacatcc ccgcgacgtg cagcatcata
     2281 tggccgatat ccagcgttgc cagcaccagc agcgaaggga tcaccgcgcc tgccagatga
     2341 tcgacaaaca cccgcacatg gcccgcaccg gaaagccgtg acgccagcac aaactcgcgt
     2401 tggcgtagtg aaatcaccaa gctgcgcacc atgcgtgaat accacgccca gtgcgacagg
     2461 gcgatggcga taattacgtt ggtcagcccg gtgccgagca cgccaaccat aaagaacgac
     2521 agaatcgagg tcgggaaggt cataaacata tcggcgacgc gcatggtggc ctgatcaacg
     2581 cgcccgccaa tcaacccggc gctgccgcca ataaccagcc ctaatgtcag caccagcagc
     2641 aggcaggcca ttaccgaacc gagcgacatg cgggtcgctg ccatcagccg cgagaaaata
     2701 tcgcgaccta agtgatcggt gcccagccag tgctgcgcat ccggcgaaag caggcacgac
     2761 ggcaaatcaa ttgcctgtgg atcgtacggc agccaccact ggctggtgag cgcaatcagc
     2821 gccagcaggg cgataatgat cagcgccagg cgtaccgacc agcgggaaga gaggaaaaag
     2881 ttcacgcgtg cgctccttca tgacgacgaa tgcgcgggtc cagcgcggcg ttgagcaaat
     2941 cgacaatcaa attacagacc acaaaaacca ccaccatcat cagcgtaaag cactgaatca
     3001 ccggatagtc acggttaaaa atcgccgaca ccgcatagcg cccgacgcca ggccaggcaa
     3061 agatgttttc gataatcatc gtcccgccaa tcaattcgcc aatgtgcatc cccacggcgg
     3121 tgatcatcgg cagcgaggca ttgcgcagaa tatggcggcg ttcggtctgt ttgtcattca
     3181 ggccgcgtag ccgcgcccag gtgacgtgac gctgaccggc gacgtccagc atactggcgc
     3241 gcagcagtcg cgcgttaatc gccagcgaca taaaggcgat ggaaaccgca ggtaaaatga
     3301 tgtgctgcca gccgccgtag cccatcgcgg gtagccattg cagatacacc gaaaacgcca
     3361 tcactagcaa aaatgccagc cagaagttgg gcatcgacac gccaagaaac gcgataaagc
     3421 gcacggcgaa atccggcaaa cgatcgcggt ggcgcgccgc ccagataccg agcggtacgg
     3481 aagtgagcag aattaatacc agcgccgcac ctgcaagttc cagcgtggcg ggcaggaagt
     3541 tcagcatatc gtccagtacc gggcgttggc tggcgaatga gataccaaag tcaagatgca
     3601 gcgccttcca caaccaggta ccgtactgga cgtacagcgg ctgatccagc ccgagcatgg
     3661 tgcgggtaga ggccagcatc tccggcgtcg gcggcaggtt agataaacgc agataatcga
     3721 gcgccgggtc gccggtaccg aggcgcagca tcagaaaaat gatcaccgag gcggcgagca
     3781 ccatcgggat cagcagcaga aagcggcgta atacgtaacg caacattaag gtttcaccgg
     3841 tttaatctgt tcgaacggaa tttcggtggc gatcggcgcg taggggatat tacccagctc
     3901 cggttttgat accaccatca tcgagatgta gctgataggc agataaaccg cctcgtcatg
     3961 cagacgggtc agaatgtcgc gatacagcgc ctgacgttgc gtttcgtcat gggtcgccag
     4021 cacttcgccg atctctttat caatcagcgg gttgtcggct aatccttgct gtgcctggaa
     4081 gtcagcgtgc gacggtacgc gcattgaact caggaaggcg tgtggatcgt atggcgcgcc
     4141 ccaggtgcgg tggaaaatca tgccaaaacg accgtcgcgc tgacgagcat agatactgct
     4201 ctcttcttcg ccaatcagcg agacatctgc gccaatctgg cgcatatcag cctgaatgat
     4261 ttccgccatc gatttgctta acgcatcggt gccgatgaac gacagttcaa tgcgcagcgg
     4321 ctgaccattt ttctcgcgga tgtctttgcc cgcaggcagc gtccaaccgg ctttttccag
     4381 caactctttc gctttctgcg gatcgtactg gcgcggtttc aggccgagat tagcgtaagg
     4441 cacagaaggg gcaaacaggg tgtcggcgac ctgctgggtg ccatataacg cgttatcaat
     4501 cagcgatttt ttgtttaccg cgtaattaag agcttcacgt actgccagct cgttggtggg
     4561 ggctttggcg gtattgagcg ccagcatcac ggtttcgatc ggctgtgaca gttgggtgtg
     4621 gtaagccgga ttctggctaa agcgggcgaa ggtatcgagc ggtaataacc cttcgtttcc
     4681 gtacagcagg tcgatatcgc cagtttcaaa cgccaccgcg cgggtagtcg ggtccgggat
     4741 gacgttaaag gtgatctttt taatcgctgg cttttcgccc cagtagtttt cgttacggac
     4801 gaagacatcg tactgattca gtttcgattc ctgcaaaatc cacggtccgg tgccaatcgg
     4861 cgctttaatt ccgttcatgg tttcatggtt tttaaactgc gagggagcga taaaacggaa
     4921 aggacggggc agggccagtt cttgcaggaa aggatagtag gcgcttttca gggtaatttg
     4981 cagctctgtt ttactgagtg ctttaacatc aacaatctgg tttgccagct ccagccaggc
     5041 gtgacgttga cggttatcga gcactgcgcg gaagttttct gccgccgcct cggcatcgaa
     5101 cggttcaccg ttggagaatt tcacgtcatc acgcagggtg aaggtccagg ttttaccatc
     5161 ttctgaatga gtccagcttt ttgccagcca cgggatcacc gaaccgtctg cctgatattt
     5221 caccaatggt tcataaacca tgctctgggc gaacatctgg ttaggcgtgt aaaggtgcgg
     5281 gtttagtggc ccgacattca ccggccaggc ggtggtgatt tcatctggtg cagcggcatg
     5341 gacgataaaa gacgcacaag ccagcagcgc aaatagagtg cggcggagtg tggagagcat
     5401 ggtaacccca atggattaaa atagatggcg gaaataagta tgacgatttt aagtattcgt
     5461 catactgatt acctgttctg gatcaataga tgggcagtcg ggtgggcgga tcagttaact
     5521 gaatcgatcc attgcacact gtcggcggtg agcgtaaagg gggtgggggt gcagatcgca
     5581 aggcttaaat tttctagctg acaatggctg actgacagcg aggagtgata ggtgctatct
     5641 acgctgacga tttgccaggc gctgccgcca cgctgtttaa cgatggcttc tttgcgcgtc
     5701 cagatgcgcc agaacatttc cagttgctga tcaggatgca cggcgtccat ttcagcgtgt
     5761 tccccgaggc tgaacacggc gttcgccagc cagcgccagt tggcgcgcgg gcgaatcact
     5821 tcgatatcgc agccgacttc gccttcatcg ctcaacagca gagcgatatc gtcaccgcta
     5881 tggcttaagt tgaaccatag cggcgtttcc ggcgcgaatg caggtttgcc ttgttcgcca
     5941 tagatgatct ccggtagcgg ggaaagcgtg tgcgaaagca atgcacgccc cgccagccag
     6001 cgttcgcgtc gtggaccttg cggtgcttgc tcgcgtaaac ccggtggcag tggagctgcg
     6061 cttaaggtcg aaactttccc cagaactatc cgatacatat cagggccaac gtttaatgga
     6121 aaatgaaagt gcgtatcgta tcacttgtcg cctcatcccg gtaaccgact tttcggtctg
     6181 cccggcccca gtaaaatcgc cagtttgctg ccacctttcg tcgtttccat ccagatttta
     6241 cacacgctgg ttaatggcac cgaaagtagc atccctaccg ggccgagcag ccatccccag
     6301 attaacaatg aaagaaatac caccatggtg gacatcccca ggcgatggcc catcatccgt
     6361 ggttctaaaa tattgccgat gaccatatgg accactaaaa acaatgcgcc gaccagaata
     6421 cattcgtaaa caccattaaa cagcagcacc tgaatcattg gcggtacggc ggaaattacc
     6481 gcgccgatat tgggcacgta gttgagcaaa aacgccagta ctgcccacat cagcgcaaac
     6541 tgtacgccca ttagcgccag ccccagccag acgatgacac ctgtccataa actgagtagc
     6601 gtctttaacg ccagataatg cgaaacgcct ttaagtgcgc ggtgtaatcc tgcgatgtga
     6661 atctgtgggt tattcagcgc aaaacgcatt ttgtaaggga cgtggcgcac ttcaaacagc
     6721 ataaaaacta cggtcatcac cagcaaaagc acgctcgcca ttgccccgga aagcccggtc
     6781 attagcgctg tggtgaaggt aaccactttt tccgagtcca tccgctgcag cattcgctcc
     6841 ggcgacatat gcaaattaag aaaaggcaac atctcctgca atttaaaaag gttgcgcgtc
     6901 agctccttat taaacttcgg cagcatagag ataaattcgt taaacgatgc cgccagtacg
     6961 ccgaccagcg cggttagtgc gatcagcatc accaccacta caatcgtaat ggcaacgggg
     7021 cgttgtactc cccgacgaat aaaccaggta acgagcgggt tgaggacgat ggcaaaaaac
     7081 agcgccagta acagctgcac aatgatatct gccgctgcgt gaatgcccgc gaggatcact
     7141 accagcgagg ccaacttgag cagaatgtgc atgcccgttt tatcgggttg aggggtttcc
     7201 attggggctt ccttgtgact ttttgtgtta agtgtagcgg gagtcacgcc agcaatattc
     7261 tgattcttac tgctgatttt cacgtcagca catggtaaaa atgaaacact gttgtaaaaa
     7321 tgtggtgatc ctcatgcccg aacccgtagc cgaacccgcg ctaaacggat tgcgcctgaa
     7381 tttgcgcatt gtctccattg tcatgtttaa cttcgccagc tacctcacca tcgggttgcc
     7441 gctcgctgta ttaccgggct atgtccatga tgtgatgggc tttagcgcct tctgggcagg
     7501 attggttatc agcctgcaat atttcgccac cttgctgagc cgccctcatg ccggacgtta
     7561 cgccgatttg ctgggaccca aaaagattgt cgtcttcggt ttatgcggct gctttttgag
     7621 cggtctgggg tatctgacgg caggattaac cgccagtctg cctgtcatca gcctgttatt
     7681 actttgcctg gggcgcgtca tccttgggat tgggcaaagt tttgccggaa cgggatcgac
     7741 cctatggggc gttggagtgg ttggctcgct gcatatcggg cgggtgattt cgtggaacgg
     7801 cattgtcact tacggggcga tggcgatggg tgcgccgtta ggcgtcgtgt tttatcactg
     7861 gggcggcttg caggcgttag cgttaatcat tatgggcgtg gcgctggtgg ccattttgtt
     7921 ggcgatcccg cgtccgacgg taaaagccag taaaggcaaa ccgctgccgt ttcgcgcggt
     7981 gcttgggcgc gtctggctgt acggtatggc gctggcactg gcttccgccg gatttggcgt
     8041 catcgccacc tttatcacgc tgttttatga cgctaaaggt tgggacggtg cggctttcgc
     8101 gctgacgctg tttagctgtg cgtttgtcgg tacgcgtttg ttattcccta acggcattaa
     8161 ccgtatcggt ggcttaaacg tagcgatgat ttgctttagc gttgagataa tcggcctgct
     8221 actggttggc gtggcgacta tgccgtggat ggcgaaaatc ggcgtcttac tggcgggggc
     8281 cgggttttcg ctggtgttcc cggcattggg tgtagtggcg gtaaaagcgg ttccgcagca
     8341 aaatcagggg gcggcgctgg caacttacac cgtatttatg gatttatcgc ttggcgtgac
     8401 tggaccactg gctgggctgg tgatgagctg ggcgggcgta ccggtgattt atctggcggc
     8461 ggcgggactg gtcgcaatcg cgttattact gacgtggcga ttaaaaaaac ggcctccgga
     8521 acacgtccct ggggccgcct catcatctta aaacttactg aataaccagc gtattaatga
     8581 tgttttctgc ggtggtttgc gctttttgct gatcgtcagc tggcagcgta atttgcatgg
     8641 tcagcagttg attacccacg ttacccagaa taacggaaga gtacgccgtc tggcctttcg
     8701 cggagataat actgtctaac tgctgcattt tgtgaccttt cagctcaatg gctttattgg
     8761 ttaccacttg cagctgcgga tcgcggctac gttgctgatc ttccagacgc ttcgccaaca
     8821 ccgccagatc ttctttcgga tcatcgccca tgatgacgat gactgctttc tgcccggtgg
     8881 cgtcggacca gacatgcatg ttattggcct gcgttcccag cttaccgctc tggtcggtca
     8941 tatccgctgg cagcgagaaa cttaacttgc catcaagcag gttgacggga ttcccggtag
     9001 cgttactttc cgcgaccgaa ccctgcgccg tagcgttagt gtctttatca tcacaggccg
     9061 caagccccat aaccagcagg ccaattccga catatttaac cagattgcgc attgacttct
     9121 tcctttcgat aaacggccat aacggctcat tcatccatct tatcacaact ctgataacga
     9181 acctttaact cgcctgcaaa gcgttgattt cggatttatc tgccagtctt ttcaacagca
     9241 tattgagtaa tacgccatac attggcagga agaaaacgat actgattaac actttgaaac
     9301 agtaatcgac cagcgcgatt tccatccagt gttcagccat aaaggcatcc gggctacgcc
     9361 agaaggcaat gaagaaaaag gccagcgtgt cgctgacgtt accgaacagt gtggacgctg
     9421 tcggtgccag ccaccagcgg cgactctgac gcaggcggtt aaaaacgtgc acgtcgagga
     9481 tttgccccag cgcgtaggcc atgaaactgg cggtggcgat acgggcgaca aacaggttga
     9541 agtgggcgag tgcgccgaat ctctgccagg aacccatata gaatagcgac gagatgacgt
     9601 aggagattaa taacgcaggg atcattaccg cgaagataat gcgtcgggcc agcggtgcgc
     9661 caaaaatacg cacggtcagg tcggtagcaa gaaaaataaa cggaaagcta aacgcgcccc
     9721 aggtggtatg gaaaccaaaa atggagacgg gaagctgaac cagatagtta ctggaggtga
     9781 tcaccagaag atgaaataac gataaccaga acaacgcctt atagcgttga gtttgcgaga
     9841 aaacgttcat attgtacctt tttgattaac cattggggtg agggaaccca atacgtacga
     9901 cacgtctgtt accttaacat aataacgtgc ttaccctttt ttcgagccgc cgcatgatac
     9961 tgctttacgt tgacaatgca atggttaatt ttcacgcaat cgttaaccta gtttgcttac
    10021 ggactcacag ggcgtaaact agcgccgttt ttttaagtga tgagaagaaa atgaccgatc
    10081 tcttttccag ccctgaccac acactcgacg cgcttggcct gcgctgcccg gaaccggtga
    10141 tgatggtgcg caaaaccgtg cgcaatatgc agcctggcga aacgttgctg attatcgccg
    10201 acgatccggc cactacccgc gatattcctg ggttttgtac ctttatggaa cacgaactgg
    10261 ttgcgaaaga gacggatgga ctgccttatc gttatttgat tcgtaaaggc ggttgatagg
    10321 ggctgattgg cttcgatgcc gccttttcct caccccctaa ccctctcccc agaggggcga
    10381 ggggaccgat cgcgctcaat gttgcgatcg gtttgcctta tctcctgcgc aacaacctta
    10441 acgcattcac cgtcaccagc accgtcgcac ccgtatctgc cagcactgcc agccacaacc
    10501 cggtcatccc taaaagcgtg gtgacgagga agatcccttt cagtcccagc gcaatggtga
    10561 tgttctggcg gatattggcg tgagtggcgc gtgccagttc aatcatttgc accaggccgc
    10621 gcaggtggtt atgggttaat gctgcgtcgg cggtttccag cgccacgtct gtgccgctac
    10681 ccatagcaat cccgatggcg gcagctttca tcgctggcgc gtcgttaata ccatcaccga
    10741 ccatcgccag gggcgcatgt tgattcagct tggtcaccgc tttgacttta tcttccggca
    10801 ataggcccgc tttaaactcc agccccagct ccccggcaat tgccgccgct gcgcgtggat
    10861 tatcgccggt gaggatcact cctttaaccc ccagcgcgtt cagttcactg atggcagttg
    10921 cagcgtcggc gcgcagggta tcctgcaatg caatgatacc cagcacgtca tcattacgta
    10981 ctaccagcac aaccgtttgc ccggcgcttt ccagttcatt aatcaaacca gcaaatgcat
    11041 cagcgggatg tttcccggca gcgcatatca acacgcgctc accattaacc tgcgcttcaa
    11101 tgccagaccc gaccagtgcc cgctgtgatt cggcggtggg aatggcgagt tcagcaacct
    11161 gtgcttcgcg tacgatggct tgcgccagtg gatgcgtcgc gccttgctcg accgccgccg
    11221 ccagtgtcag cagttcagat tcactaatac ccgttgccgg atgaatcgcg gtaacgcgcg
    11281 gtttaccgac ggtcagcgta ccggttttat caaacgccac ttgggtaaca cgacctagtt
    11341 gttccagcgc cgcaccgcct ttaattaacg ccccacgacg cgctgctgct gccagcccgg
    11401 aggttatcgc cgcaggcgtt gagataacta acgcacacgg gcaaccaatc agcagcagcg
    11461 tcagaccttt ataaatccac tcctgccagc tggcggcaaa cagcagcggc ggcactagcg
    11521 ttaccagcag ggcgacggcc ataatcgctg gcgtataaat acggctgaaa cggtcgataa
    11581 accgctcaat gggggcgcgg cgctcttcgg cttcttcgat cagtttcaga atccggtcaa
    11641 tggcgctggc acccggttct gacagcactt ccagcgttac cagacggtct acgctggtgg
    11701 ctcctgcagg aactttatcg cccgtcgcgc gctccaccgg aatggattct ccggtcaggg
    11761 cgctttcatc aaaactggca aacggtgaga gcagtttacc gtcggcaggc aaacgcccac
    11821 ctgcggcgac ttcaatcaca tcgccaggcc gcaggctgtt aatcgccacc tcttcccgct
    11881 caccgttacg caggcgcgtg gcggtttctg gtttcagcgc cattaacgcg ctaacgccct
    11941 gacgcgcgcg gctggcggcc cagccttcca gtcgttcacc aatcaaaaac agcagcaaca
    12001 ccatcgcagc ttcagccgtt gcgccaataa acagtgcacc aatagcggct acgctcatta
    12061 aggtttcaat ggcgaagtag ctgccggatt tgatcagccg taatgcctga cgagcaatcg
    12121 ggtacagccc aactagcgtg gtcgcgataa acgccagttg cccgaacgga tgattgaact
    12181 gctccagacc ccagctgatt gccatcatca cgataagcgt aatcagcggc agattctctt
    12241 tcaggcgcga tgcttgtggc tcgtcggcgg cctgctcatc gcgcagggaa tagcccgctt
    12301 tttgcaccgc agattcaacc tgggcacgga tgtcattgtc ggcatcaacc accagttttt
    12361 cggtggcgaa caacacctgc acctgattca cgcctgcaag ctggcgcacg gcattttcta
    12421 ccttgcgcgc acaggcggcg cagtccatgc cgctgacttt ccagctatag cgggtgccgg
    12481 agacgttttc agagagcgtt ggcgagctgg agcatgcgcc gtcgcagcaa cagtcgttgg
    12541 cgttctgtac cgtggttagc ggtttgaacg cagcaaattg aggggctttc ttgccgtgat
    12601 tgtcaggtgt cgacatggca tcctccggtt aagttttttc tcattaaccg aaggatacac
    12661 tctggagtcg actccagagt caagttttat cagagataca gtgagcggac gatgaggaag
    12721 tgaccggcaa agtagcaggc cgcagcaatc gcgttatccg cacggaagcg tcggcgatag
    12781 tggctcccca gccagacaaa gttactgata aacagcaacg aagcgccaac aaacgcagag
    12841 agcgccggag cggtcggacg gaagaaccac agttcacctg ccagccacac catcaccagc
    12901 gtcatgccga taaacgtgca gataggccaa cggtactctt ccagccgcgt ccagataatc
    12961 gccagtagca gcgcacccag aaccagcaac accagtggca gaggccagaa gaaagagagc
    13021 gtcatctgac tggcgaaata gatggtgtac agcaggtgcg agaggaaaaa cgcgccgatg
    13081 gcgtacatca gacgttgacg tggcaacagg gttagcgcat cgcccagcag tgaggcgcac
    13141 agtcccgcca gcaccagata gctaatggcg tcgaacatcg gtgcttgcca ggccagtaac
    13201 agcaggagaa gaagggttaa cggtttaaac acccagcgtt gccaggttgg cccacgatac
    13261 gatgcatcca cagatagcca tgcggaaaga cagacagcga taaacgacca aagcatctta
    13321 gttccttgaa ttcgttattt tttcgtaagc ggaatgccta cggtcttgct tccagtttag
    13381 tggcgagggt ggacggatga caacaccata actggcaggg tatgcttaat tccgcatttt
    13441 ccgatgaggg ataaagatga gtaaaccacc tcttttcttt attgttatca ttggcttaat
    13501 tgtcgtcgcc gcatcgtttc gttttatgca acagcgacgg gaaaaagctg ataatgatat
    13561 ggctccgctc cagcaaaagc tggtggtggt gagcaacaag cgggaaaaac cgattaacga
    13621 tcgccgttcg cgccagcagg aagtgactcc ggcaggcacc agtatgcgct atgaggcaag
    13681 cttcaaaccg caaagcggag gaatggagca gacgtttcgc ctcgacgccc agcagtacca
    13741 cgccctgaca gtgggcgata aaggtacgct gagctataaa ggaacgcgct ttgttagctt
    13801 tgttggcgaa caataaggtt attttttaac tttaaatttc ttctgccaga ccagcagttc
    13861 aaacacgccg aaaaggaaaa tccgcacctt ttcggcggtg gtcatctgcg ggccatcttt
    13921 cggtaaagtg gatttcaata gcgccagctg cataccatgc atcagcacgg taaaaatcag
    13981 cgccacgtta acgaaaatat ttagcgggcg cgggaaggga tgcaccagat tgaggattaa
    14041 aaatccccaa acgcagagca ttaacaaacg accaatatta atcagcatca ctttctcctt
    14101 gtgcttcgtg ttgatacagc cgataagcca cctgacccgc cactttttcc cgatgtaatg
    14161 accagtttgc cggaacagtg ggcagaccgt tttcgacttc gctttcgaca taaatcaggg
    14221 cttcgtcagc cagccagccg ttatcttcca gtaaatttat cgtctcttct aacaagccac
    14281 ggcggaacgg tggatcgaca aacacgatat tatgcggtgt gcctttttgc gccaggaatg
    14341 acattgcgtt gctgttcacc acgcgtgcat tgcctgcttt tagtgtcgcc agattcttaa
    14401 ttaactgctg agaaaccacg cgatccatct caatcaacgt tgcccccgca gcgtagcgcg
    14461 ataacgcttc cagccccagc gcgccgctcc cggcgaagca atccagacat tgggcgtcaa
    14521 caatgaccgg agccagccag ttaaacaacg tttcgcgtac gcggtcggtg gtggggcgca
    14581 gacctgggct atcaggaacc gggagtttac ggcctcgcca ctgcccgcca ataatgcgga
    14641 tttggccgct gccggaatga ttcggttttt tcatgataaa ttgctcaatc cgccagataa
    14701 cacatacttg caggcggtga tgtgaattaa gttaagtgat agactatttc atcatttttt
    14761 tagctgctat gtacatagcg ttaacgctgt gccatgaagc aacagcgagg agtgtagtcg
    14821 caaatggcga aagaaaaaaa acgtggcttt ttttcctggc tgggctttgg tcaaaaagag
    14881 cagaccccgg aaaaagagac agaagttcag aatgaacaac cggttgtaga agaaatcgtt
    14941 caggcgcaag agcctgtgaa ggcttctgaa caagccgttg aagagcagcc gcaggcgcat
    15001 actgaagccg aggcggaaac ttttgctgcc gacgttgtgg aagtcactga acaggttgtt
    15061 gaaagtgaaa aagcgcagcc tgaagcggaa gttgttgcac agccggaacc ggtcgtagaa
    15121 gaaacgccgg agccagtggc tatcgaacgt gaagagctgc cgttgccgga agacgtcaac
    15181 gccgaagagg tttcgccaga agagtggcag gctgaagcgg aaaccgtaga gattgtcgaa
    15241 gcggcagaag aagaagcggc taaagaagaa atcaccgacg aagagctgga agcacaggcg
    15301 ctggctgccg aagcggcaga agaggcggtg atggtagttc ctccggcaga agaagagcag
    15361 ccggttgaag aaattgccca ggagcaagaa aaaccgacca aagaaggctt cttcgcgcgc
    15421 ctgaaacgca gcctgttaaa aaccaaagaa aatctcggtt ccggatttat cagcctgttc
    15481 cgcggtaaaa aaatcgacga tgatctgttt gaagagctgg aagaacagct gttgatcgcc
    15541 gatgtgggcg tggaaaccac acgtaaaatt atcaccaatc tgacggaagg cgcatcccgc
    15601 aagcagcttc gtgatgccga ggcgctctat ggcctgctga aagaagagat gggcgagatt
    15661 ctggcgaaag tcgatgagcc gctgaatgtt gaagataaaa cgccgttcgt gatcctgatg
    15721 gtgggcgtca acggtgtggg taaaaccacg acgattggta agctggcgcg tcagtttgag
    15781 cagcagggta aatcggtgat gctggcggcg ggcgatactt tccgtgcagc tgcggttgaa
    15841 cagcttcagg tctggggtca gcgcaacaat attccggtga ttgcccagca taccggtgcg
    15901 gattccgcct ctgttatctt cgacgccatt caggcagcta aagcgcgtaa tatcgacgtc
    15961 ctgattgccg ataccgccgg acgtctgcag aacaaatcgc atctgatgga agagttgaag
    16021 aaaatcgtcc gcgtgatgaa gaaactcgac gttgaagcgc cgcatgaagt tatgctgact
    16081 attgatgcca gcaccgggca gaacgcggta agccaggcca aactgttcca cgaagctgtt
    16141 ggcttaaccg gcatcacgct aacgaaactg gacggcacgg cgaaaggcgg ggtaattttc
    16201 tcggtggctg accagtttgg tatccctatc cgctacattg gtgtcggcga acgtattgag
    16261 gatttgcgtc cgtttaaggc ggacgacttt atagaggcac tttttgcccg agaggattaa
    16321 caatgattcg ctttgaacat gtcagcaagg cttatctcgg tgggagacag gcgctgcagg
    16381 gcgttacgtt ccatatgcag ccgggtgaga tggcgtttct gaccggtcat tccggcgcag
    16441 ggaaaagtac cctcctgaag ctgatctgtg ggattgagcg gcccagcgcc gggaaaatct
    16501 ggtttagcgg ccatgacatc acgcgtctga aaaaccgtga agttccgttt ctgcgtcgcc
    16561 agattggcat gattttccag gatcaccatc tactgatgga ccgtactgtc tacgataacg
    16621 tggcgatccc gctgattatc gctggtgcca gcggtgacga tattcgtcgc cgggtgtcgg
    16681 cggcactgga taaagtcggg ctactggaca aagcgaagaa cttccctatt cagctttccg
    16741 gcggtgaaca acagcgtgtt ggcattgccc gcgcggtggt gaataagccc gcggtactgc
    16801 tggcggacga accgaccggt aacctggacg acgcgttgtc ggaagggatt ctacgtctgt
    16861 ttgaagagtt taaccgcgtt ggggtaaccg tattgatggc aacgcacgac attaacctga
    16921 tttcacggcg ttcctatcgc atgctcaccc tgagcgatgg tcacttgcat ggaggcgtgg
    16981 gccatgaata agcgcgatgc aatcaatcat attcggcagt ttggtgggcg tctggatcgc
    17041 ttccgtaaat cggtcggcgg ctcaggcgac ggcggtcgta acgcaccgaa acgcgcgaaa
    17101 tcctcgccaa aaccggtaaa tcgcaaaacc aacgttttca acgaacaggt gcgctatgcc
    17161 ttccacggcg cattgcagga tctgaaaagc aaaccgttcg ccacgttttt aacggtgatg
    17221 gttatcgcca tctccctgac gctgcccagc gtctgttata tggtgtacaa aaacgttaat
    17281 caggcggcga cgcagtatta tccgtcaccg caaatcactg tttacttgca aaaaacgctg
    17341 gacgatgacg ccgctgcggg cgtggtggca cagttgcagg ccgagcaagg cgtggagaaa
    17401 gtgaactatc tttctcgtga agacgcactg ggcgagttcc gtaactggtc tggttttggt
    17461 ggtgcgctgg atatgctgga agaaaacccg cttccggcag tggcggtggt gatcccgaaa
    17521 ctcgattttc aggggacgga atcactcaat acgctgcgtg atcgtatcac gcagattaac
    17581 ggcattgacg aagtgcggat ggatgacagc tggtttgccc gtctggcggc gttgaccggg
    17641 ctggttgggc gcgtttcggc gatgatcggc gtgttgatgg tggcggcagt gttcctcgtc
    17701 atcggcaaca gcgtacgtct gagcatcttt gctcgccgtg actccatcaa cgtgcagaaa
    17761 ctgattggtg cgacggatgg tttcatcctt cgcccgttcc tgtacggcgg tgcgctgctg
    17821 ggattttctg gcgcattgtt gtcattaatt ttgtcagaaa ttctggtact gcgtttgtcg
    17881 tcagcggtgg cggaagtggc acaggttttc ggaacgaagt ttgatatcaa tggtttatcg
    17941 ttcgatgaat gcctgctatt gctgctggta tgctcgatga ttggctgggt ggcagcgtgg
    18001 cttgccacgg tacaacattt acgccacttt acgcctgaat aataaaagcg tgttatactc
    18061 tttccctgca atgggttccg tagcagggaa agagaccccg ttgtctcttc ccggtatttc
    18121 atctctatgt cacattttgt gcgtaattta ttcacaagct tgcattgaac ttgtggataa
    18181 aatcacggtc tgataaaaca gtgaatgata acctcgttgc tcttaagctc tggcacagtt
    18241 gttgctacca ctgaagcgcc agaagatatc gattgagagg atttgaatga ctgacaaaat
    18301 gcaaagttta gctttagccc cagttggcaa cctggattcc tacatccggg cagctaacgc
    18361 gtggccgatg ttgtcggctg acgaggagcg ggcgctggct gaaaagctgc attaccatgg
    18421 cgatctggaa gcagctaaaa cgctgatcct gtctcacctg cggtttgttg ttcatattgc
    18481 tcgtaattat gcgggctatg gcctgccaca ggcggatttg attcaggaag gtaacatcgg
    18541 cctgatgaaa gcagtgcgcc gttttaaccc ggaagtgggt gtgcgcctgg tctccttcgc
    18601 cgtccactgg atcaaagcag agatccacga atacgttctg cggaactggc gtatcgtcaa
    18661 agttgcgacc accaaagcgc agcgcaaact gttcttcaac ctgcgtaaaa ccaagcagcg
    18721 tctgggctgg tttaaccagg atgaagtcga aatggtggcc cgtgaactgg gcgtaaccag
    18781 caaagacgta cgtgagatgg aatcacgtat ggcggcacag gacatgacct ttgacctgtc
    18841 ttccgacgac gattccgaca gccagccgat ggcaccggtg ctctatctgc aggataaatc
    18901 atctaacttt gccgacggca tcgaagatga taactgggaa gagcaggcgg caaaccgtct
    18961 gaccgacgcg atgcatggtc tggacgaacg cagccaggac atcatccgtg cgcgctggct
    19021 ggacgaagac aacaagtcca cgttgcagga actggctgac cgttacggtg tttccgctga
    19081 acgtgtgcgc caactggaaa agaacgcgat gaaaaaactt cgcgccgcta ttgaagcgta
    19141 atttccgcta ttaagcagag aaccctggat gagagtccgg ggtttttgtt ttttgggcct
    19201 ctacaataat caattccccc tccggcaaaa cgccaatccc cacgcagatt gttaataaac
    19261 tgtcaaaata gctattccaa tatcataaaa atcgggtatg ttttagcaga gtatgctgct
    19321 aaagcacggg tagtcatgca taaaacgaaa taaagtgctg aacaacaaca tcacaacaca
    19381 cgtaataacc agaagaatgg ggattctcag gatgaacata aagggtaaag cgttactggc
    19441 aggatgtatc gcgctggcat tcagcaatat ggctctggca gaagatatta aagtcgcggt
    19501 cgtgggtgca atgtccggtc cggttgcgca gtacggtgac caggagttta ccggcgcaga
    19561 gcaggcggtt gcggatatca acgctaaagg cggcattaaa ggcaacaaac tgcaaatcgt
    19621 aaaatatgac gatgcctgtg acccgaaaca ggcggttgcg gtggcgaaca aagtcgttaa
    19681 cgacggcatt aaatatgtga ttggtcacct ctgttcttca tcaacgcagc ctgcgtctga
    19741 catctacgaa gacgaaggca ttttaatgat caccccagcg gcaaccgcgc cggagctgac
    19801 cgcccgtggc tatcagctga tcctgcgcac caccggcctg gactccgacc aggggccgac
    19861 ggcggcgaaa tatattcttg agaaagtgaa accgcagcgt attgctatcg ttcacgacaa
    19921 acagcaatac ggcgaaggtc tggcacgagc ggtgcaggac ggcctgaaga aaggcaatgc
    19981 taacgtggtg ttctttgatg gtatcaccgc cggggaaaaa gatttctcaa cgctggtggc
    20041 gcgtctgaaa aaagagaata tcgacttcgt ttactacggc ggttatcacc cggaaatggg
    20101 gcaaatcctg cgtcaggcac gcgcggcagg gctgaaaact ggaaggtgtg gctaacgttt
    20161 cgctgtctaa cattgcgggc gaatcagcgg aagggctgct ggtgaccaag ccgaagaact
    20221 acgatcaggt tccggcgaac aaacccattg ttgatgcgat caaagcgaaa aaacaggacc
    20281 caagcggcgc attcgtctgg accacctacg ccgcgctgca atctttgcag gcgggcctga
    20341 atcagtctga cgatccggct gaaatcgcca aatacctaaa agcgaactcc gtggataccg
    20401 tgatggggcc actgacctgg gatgagaaag gcgatctaaa gggctttgag ttcggcgtat
    20461 ttgactggca cgccaacggc acggcgaccg atgcgaagta attgttaatc ggcaactttg
    20521 ggttgccgcc aaattgctaa tatcgaatac gttgcttcat gccggatgcg gcgtaaacgc
    20581 cttatccggc ctacaagatc caaagaaatc agtaaattgc agcacacatt ataggcctga
    20641 taagcgtagc gcatcaggca atttctcgtt ggtcatcact tttgaaatca acttgacgat
    20701 taacgcttct cccagccgcc ctgcagtgcc gtaaatccca gtgcctgcat aaacgccgtc
    20761 atcacaccgc gatcttccac gcctgcatcc gccatccacc agcatgaaac gccaggattg
    20821 ttacgcaaaa cctcttccag cagatattgc cccacaccgc gacggcgggt gacttcccgc
    20881 acgcgtagag aatccagtgc tccctcggtg ccgcttaagg ttacccgcac ggccgcaagt
    20941 agacgctcgt taaaacgcgc ggcgtagata cggtggttat cgtcaacctg taaagaggaa
    21001 ggggaatact ccggccagat cttttgcagg tcaatccggt cctggtcgct aaagttttct
    21061 aatcgaatga tggtcagctt catgggtaac ccgtgtaaat cacaaaagtg gaaccagtgt
    21121 agcgaaataa tttaatcgga ggctttctct tttttatttc ttttggcagg tgattaattt
    21181 tttaacagca ataattacaa agttaaaaca ttagagaatg aaaaatgtcc agcataatcc
    21241 cctgaatgat agtgaattat tccgcccttt gtgccgttat tttatgctga caaaggcact
    21301 tttttctgtt tatctatcaa taaattcaga atattatctg ttcttaatcg actgaaaaat
    21361 agggatttta atcgctatca tcacaaaata ctgcgctaac cccttaatca gacaggcaaa
    21421 aacagtgcag tataaaaaaa gaacagtctg atttgttaac acataaaaac aaagcaacac
    21481 aacatcacga atggggattt ttgactatga aacggaatgc gaaaactatc atcgcaggga
    21541 tgattgcact ggcaatttca cacaccgcta tggctgacga tattaaagtc gccgttgtcg
    21601 gcgcgatgtc cggcccgatt gcccagtggg gcgatatgga atttaacggc gcgcgtcagg
    21661 cgattaaaga cattaatgcc aaagggggaa ttaagggcga caagctggtt ggcgtggaat
    21721 atgacgacgc ctgcgacccg aaacaagccg ttgcggtcgc caacaaaatc gttaacgacg
    21781 gcattaaata cgttattggt catctgtgtt cttcctctac ccagccagcg tcggatatct
    21841 acgaagacga aggtattctg atgatctcgc cgggagcgac caacccggag ctgacccaac
    21901 gcggttatca acacattatg cgtactgccg ggctggactc ttcccagggg cctacggcgg
    21961 caaaatacat tcttgagacg gtgaagcccc agcgcatcgc catcattcac gacaaacaac
    22021 agtatggcga agggctggcg cgttcggtgc aggacgggct gaaagcggct aacgccaacg
    22081 tcgtcttctt cgacggtatt accgccgggg agaaagattt ctccgcgctg atcgcccgcc
    22141 tgaaaaaaga aaatatcgac ttcgtttact acggcggtta ctacccggaa atggggcaga
    22201 tgctgcgcca ggcccgttcc gttggcctga aaacccagtt tatggggccg gaaggtgtcg
    22261 gtaacgcatc gctgttgaac attgccggtg atgccgccga aggcatgttg gtcactatgc
    22321 caaaacgcta tgaccaggat ccggcaaacc agggcatcgt tgatgcgctg aaagcagaca
    22381 agaaagatcc gtccgggcct tatgtctgga tcacttacgc ggcagtgcaa tctctggcaa
    22441 ctgcccttga gcgtaccggc agcgatgagc cgctggcgct ggtgaaagat ttaaaagcta
    22501 acggtgcaaa caccgtgatt gggccgctga actgggatga aaaaggcgat cttaagggat
    22561 ttgattttgg tgtcttccag tggcacgccg acggttcatc cacggcagcc aagtgatcat
    22621 cccaccgccc gtaaaatgcg ggcgggttta gaaaggttac cttatgtctg agcagttttt
    22681 gtatttcttg cagcagatgt ttaacggcgt cacgctgggc agtacctacg cgctgatagc
    22741 catcggctac accatggttt acggcattat cggcatgatc aacttcgccc acggcgaggt
    22801 ttatatgatt ggcagctacg tctcatttat gatcatcgcc gcgctgatga tgatgggcat
    22861 tgataccggc tggctgctgg tggcggcggg attcgtcggc gcaatcgtca tcgccagcgc
    22921 ctacggctgg agtatcgaac gggtagctta ccgcccggtg cgtaactcta agcgcctgat
    22981 tgcgcttatt tccgcaatcg gtatgtccat cttcctgcaa aactacgtca gcctgaccga
    23041 aggttcgcgc gacgtggcgc tgccgagcct gtttaacggt cagtgggtgg tgggccatag
    23101 cgaaaacttc tctgcctcta ttaccaccat gcaggcggtg atctggattg tcaccttcct
    23161 cgccatgctg gcgctgacga ttttcattcg ctattcccgt atggggcgcg cctgtcgtgc
    23221 ctgcgcggaa gatctgaaaa tggcgagcct gcttggcatc aacaccgacc gggtgattgc
    23281 tttgaccttt gttattggcg cggcgatggc ggcagtggct ggcgtactgc tcggtcagtt
    23341 ctactgcgtc attaacccct atatcggctt tatggccggg atgaaagcct ttaccgctgc
    23401 ggtgctcggt gggattggca gcattccagg ggcgatgatt ggcgggctga tcctggggat
    23461 tgcggaggcg ctctcttctg cctatctgag tacggaatat aaagatgtgg tctcattcgc
    23521 cctgctgatt ctggtgctgc tggtgatgcc gaccggtatt ctgggtcgcc cggaggtaga
    23581 gaaagtatga aaccgatgca tattgcaatg gcactgctct ctgccgcgat gttctttgtc
    23641 ctggcgggcg tctttatggg cgtacaactg gagctggatg gcaccaaact ggtggtcgac
    23701 acggcttcag acatccgttg gcagtggatg tttatcggca cggcggtggt ctttttcttc
    23761 cagcttttgc gaccggcttt ccagaaaggg ttgaaaagcg tttccgggcc gaagtttatt
    23821 ctgcccgcga ttgatgggtc cacggtgaag caaaagctgt tcctcgtggc gctgctggtg
    23881 cttgtggtgg cgtggccgtt tatggtgtca cgtgggacgg tggatatcgc cactctgacc
    23941 atgatctaca ttatcctcgg tctcgggctg aatgtggttg ttggtctttc tggtctgctg
    24001 gtgctggggt acggcggttt ttacgccatc ggcgcttaca cttttgcgct gctcaatcac
    24061 tattacggtc tggggttctg gacctgtttg ccgattgccg gactgatggc agcggcggcg
    24121 ggtttcctgc tcggttttcc ggtgctgcgt ctgcgcggtg actatctggc gatcgttacc
    24181 cttggtttcg gcgaaattgt gcgcatattg ctgctcaata acactgaaat caccggcggc
    24241 ccgaacggaa tcagtcagat cccgaaaccg acactcttcg gactcgagtt cagccgtacc
    24301 gctcgtgaag gcggcaggga cacgttcagt aatttctttg gcctgaaata cgatccgtcc
    24361 gatcgcgtta tcttcctcta cctggtggcg ttgctgctgg tggtgttaag cctgtttgtc
    24421 attaaccgcc tgctgcggat gccgctgggc cgcgcgtggg aagcgttgcg tgaagacgag
    24481 atcgcctgtc gttcgctggg cttaagcccg cgccgtatca agctgaccgc ttttaccatc
    24541 agcgccgcat ttgccggttt tgccggaacg ctgtttgcgg cgcgtcaggg ctttgtcagc
    24601 ccggaatcct tcacctttgc tgaatcggcc tttgtgctgg cgatagtggt gctcggcggt
    24661 atgggctcgc aatttgcagt gattctggcg gctgttctgc tggtggtgtc gcgcgaattg
    24721 atgcgtgatt tcaacgaata cagcatgtta atgctcggtg gtttgatggt gctgatgatg
    24781 atctggcgtc cgcaggggtt gctgcccatg acgcgcccgc aactgaagct gaaaaacggc
    24841 gcagcgaaag gagagcaggc atgagtcagc cattattatc tgttaacggt ctgatgatgc
    24901 gcttcggcgg cctgctggcg gtgaacaacg tcaatcttga actgtacccg caggaaatcg
    24961 tctcgttaat cggccctaac ggtgccggaa aaaccacggt ttttaactgc ctgaccggtt
    25021 tctacaaacc caccggcggc accattttac tgcgcgatca gcatcttgaa ggtttaccgg
    25081 ggcagcaaat tgcccgcatg ggcgtggtgc gcaccttcca gcatgtgcgt ctgttccgtg
    25141 aaatgacggt aattgaaaac ctgctggtgg cgcagcatca gcaactgaaa accgggctgt
    25201 tctctggcct gttgaaaacg ccttctttcc gtcgcgccca gagcgaagcg ctcgaccgcg
    25261 ccgccacctg gcttgagcgc attggcttgc tggaacacgc caaccgtcag gcgagtaacc
    25321 tggcctatgg tgaccaacgc cgtctggaga ttgcccgctg catggtgacg cagccggaga
    25381 ttttaatgct cgacgaaccg gcggcaggtc ttaacccaaa agaaaccaaa gagctggatg
    25441 agctgattgc cgaactgcgc aatcatcaca acaccactat cttgttgatt gaacacgata
    25501 tgaagctggt gatggggatt tcagaccgaa tttatgtggt caatcagggg acgccactgg
    25561 caaacggtac gccggagcaa atccgtaata acccggacgt gatccgtgcc tatttaggtg
    25621 aggcataaga tggaaaaagt catgttgtcc tttgacaaag tcagcgccca ctacggcaaa
    25681 atccaggcgc tgcatgaggt gagcctgcat atcaatcagg gcgagattgt cactctcatt
    25741 ggcgcgaacg gggcggggaa aaccaccttg ctcggcacgt tatgcggcga tccgcgcgcc
    25801 accagcgggc gaattgtgtt tgatgataaa gacattaccg actggcagac ggcgaaaatc
    25861 atgcgcgaag cggtagcgat tgtcccggaa gggcgtcgcg tcttctcgcg gatgacggtg
    25921 gaagagaacc tggcgatggg cggcttcttt gccgaacgtg accagtttca ggagcgcata
    25981 aagtgggtgt atgaactgtt tccacgtctg catgagcgcc gtgttcagcg ggcgggcacc
    26041 atgtccggcg gtgaacagca gatgctggcg attggtcgcg cgctgatgag caatccgcgt
    26101 ttgttgttac ttgatgagcc atcgctcggt cttgcgccga ttatcatcca gcaaattttc
    26161 gacaccatcg agcagctgcg cgagcagggg atgactatct tcctcgtcga gcagaacgcc
    26221 aaccaggcgc taaagctggc ggatcgcggc tacgtgctgg aaaacggcca tgtggtgctc
    26281 tccgataccg gcgacgcgct gctggcgaac gaagcagtga gaagtgcgta tttaggcggg
    26341 taataacacg ttgattgata aggagtcaaa agactccttt gagacaggtg acaaatgtaa
    26401 aattgcctga tgcgctgcgc ttatcaggcc tactgggtga gttgcaatat attgaatttg
    26461 cacgatcttg taggccggat aaggcgttta cgccgcatcc ggcatgaaac gatgagtaat
    26521 ctgtagagtt tgattcagac cttctatttt tccgcttatc cgtgccccat ctcccatttt
    26581 ccctcaccca cgccgtcacc gccttgtcat ctttctgaca ccttactatc ttacaaatgt
    26641 aacaaaaaag ttatttttct gtaattcgag catgtcatgt taccccgcga gcataaaacg
    26701 cgtgaattcg cgcattcggt acaacaagag agataaacga tgaaaccgtt acattataca
    26761 gcttcagcac tggcgctcgg actggcgtta atggggaatg cacaggcagt gacgaccatt
    26821 ccgttctggc attctatgga aggggaactg ggtaaagagg tggattctct ggcccaacgt
    26881 tttaacgccg aaaatccgga ttacaaaatt gtaccgacct ataaaggcaa ctacgaacag
    26941 aatttaagcg cggggattgc cgcatttcgt accggcaacg ctccggctat tttgcaggtt
    27001 tatgaagttg gcaccgccac catgatggcg tcgaaagcca ttaaaccggt gtatgacgtg
    27061 tttaaagagg cagggattca gttcgatgag tcgcagtttg tgccgacggt ttccggttac
    27121 tactccgaca gcaaaaccgg ccacttactc tcccagccgt tcaacagctc gactcccgtt
    27181 ctctattaca acaaagacgc cttcaagaaa gcggggttag acccggaaca gccgccgaaa
    27241 acctggcagg atctggcgga ctattccgca aaactgaaag cctccggtat taagtgcggc
    27301 tacgccagcg gctggcaggg ctggatccaa ctggaaaact ttagcgcctg gaacggtctg
    27361 ccgtttgcca gcaaaaacaa cggctttgac ggcacggacg cggtgctgga gttcaacaag
    27421 ccggagcagg tgaaacacat cgccatgctc gaggagatga acaagaaggg cgacttcagc
    27481 tacgtcggtc gtaaggatga atccaccgag aagttctata acggtgattg cgcgatgacc
    27541 accgcctctt ccggttctct tgccaacatt cgcgagtacg ccaaatttaa ctacggcgta
    27601 ggtatgatgc cttacgacgc cgatgcgaaa gatgcgccac aaaacgccat tatcggcgga
    27661 gccagcctgt gggtgatgca gggcaaagat aaagaaacgt ataccggcgt ggcgaagttt
    27721 ctcgacttcc tcgcgaagcc agaaaacgct gctgagtggc atcagaaaac cggttatctg
    27781 ccaattacca aagcggcgta tgacctgacc cgtgagcagg gcttttacga gaaaaaccta
    27841 ggtgcggata ccgcgacgcg tcagatgctg aacaagccgc cgttgccgtt caccaaaggg
    27901 cttcgtctgg gcaacatgcc gcagatccgc gtgattgtgg atgaagagct tgagagcgtg
    27961 tggaccggta agaagacgcc acagcaggcg ctggataccg cagttgagcg tgggaaccag
    28021 ttactgcgcc gctttgagaa atcgacgaag tcttaatcag tgtaatgccg gatgcgtttc
    28081 gcttatctga cctggcatcg cgtgtaggcc ggataagcga agcgcatccg gcacagttca
    28141 ggaattaacc gtaatgtcat catcccgtcc ggtgttccgc tcgcgctggc tgccttatct
    28201 gctggtcgcg ccgcagctca tcatcaccgt tatctttttt atctggcctg cgggcgaagc
    28261 gttgtggtac tcgctacaaa gcgtcgatcc gtttggtttc tccagccagt ttgtcggtct
    28321 ggataacttc gtcacgctgt ttcatgacag ctattatctc gactccttct ggacgacgat
    28381 aaaattcagc acctttgtca ccgtcagcgg tttgctggta tcgctgttct ttgcggcgct
    28441 ggtggagtac atcgtgcgcg gcagtcgttt ctaccaaacc ttaatgttgc tgccgtatgc
    28501 cgtggctccc gccgttgccg ccgtattgtg gatcttcctg tttaaccccg gtcgcgggct
    28561 gatcacccat tttctcgctg agttcggcta cgactggaac cacgcgcaaa atagcggtca
    28621 ggcgatgttt ctggtggtgt ttgcctcggt gtggaagcaa atcagctaca actttctgtt
    28681 cttctatgcc gcgctgcaat ccattccccg ttcgttgatc gaagccgcag ccatcgacgg
    28741 tgcagggccg attcgccgct tctttaagat tgcgctgccg cttatcgccc cggtgagttt
    28801 cttcctgctg gtagtgaacc tggtgtatgc cttcttcgac accttcccgg tgatcgacgc
    28861 cgccacgtcc ggcgggccgg ttcaggccac cacgacgctg atttataaga tctaccgcga
    28921 aggttttacc ggactggatc tggcttcgtc tgccgcacag tcggtggtgt tgatgttcct
    28981 cgtcatcgtg ctgacggtgg tgcagttccg ctatgttgaa agcaaggtgc gttaccaatg
    29041 attgagaacc gtccgtggct gacgatattc agccatacca tgctgatcct cgggatcgcg
    29101 gtgatcctct tcccgctgta cgtggcgttt gtcgcggcga cgctggataa acaggccgtc
    29161 tacgccgcgc cgatgacgct catccccggc acgcatctgc tggaaaacat ccacaacatc
    29221 tgggtgaacg gggtaggcac aaatagcgcg ccgttctggc ggatgttgct taacagcttt
    29281 gtgatggcgt tcagcattac gctcggcaaa attactgtct cgatgctctc ggcatttgcc
    29341 attgtctggt ttcgttttcc gctacgtaac ctcttcttct ggatgatttt tatcaccctg
    29401 atgctgccgg ttgaagtacg tatcttcccg acggtggaag tcatcgccaa cctgaagatg
    29461 ctcgacagct acgccggttt aacgctgccg ctgatggcct cggcgaccgc cacgtttctg
    29521 tttcgccagt tctttatgac gctgccggat gaactggtgg aagcggcgcg gatcgacggt
    29581 gcgtcaccaa tgcgcttctt ttgcgacatc gtttttccgc tctccaaaac taatctggcg
    29641 gcgctgtttg tgatcacctt tatttacggc tggaaccagt atttgtggcc gttgttgatt
    29701 attaccgacg tggatctcgg caccaccgtg gcagggatca aagggatgat cgccactggc
    29761 gaaggcacca cggaatggaa ctcagtgatg gcggcgatgt tgttaaccct gatcccacca
    29821 gtagtgattg ttttagtgat gcagcgtgcc ttcgtgcgcg gcctggtcga tagtgagaaa
    29881 taagatggca ggactgaaat tacaggcagt aaccaaaagc tgggatggca aaacctaggc
    29941 cattaaaccg ctgacccttg atgtggcgga tggcgaattt atcgtgatgg tcgggccgcc
    30001 tggctgcggg aaatcgacgc tgctgcgtat ggttgccggg ctggagcggg tgaccgaagg
    30061 cgatatctgg atcaacgacc agcgcgtgac cgaaatggag ccgaaagatc gcgggattgc
    30121 gatggtgttc cagaattacg cgctttatcc gcatatgaac gtcgaagaaa acatggcgtg
    30181 ggggctgaaa attcgcggca tgggcaagca gcaaatagcc gagcgcgtta aagaagcggc
    30241 gcgcattctg gagctggacg gtctgctcaa acgtcgcccg cgcgagcttt ccggcggtca
    30301 gcgccagcgt gtggcgatgg gacgcgcgat tgtgcgcgat ccggcggtgt tcttgtttga
    30361 tgagccactc tctaacctcg atgccaagct gcgcgtacag atgcgtcttg aactgcaaca
    30421 gctgcaccgt cgcctgaaaa cgacttcact ctacgttact cacgatcagg ttgaggcgat
    30481 gacgctcgcc cagcgagtaa tggtgatgaa cggcggcgtt gccgaacaga ttggcacgcc
    30541 ggtggaagtc tacgaaaagc ccgccagcct gtttgtggcg agttttatcg gcagcccggc
    30601 gatgaacctg ctgacaggcc gcatgaataa tgaaggcacg catttcgaac tggacggcgg
    30661 tatcgtgcta ccgctaaacg gtggctaccg tcagtatgcc gggcgtaaaa tgaccctcgg
    30721 cattcgcccg gaacatattg cgctaagctc gcaagcagaa gctggtggtg cgactggcgc
    30781 atcaggagcg cccgacggca ggcagcacgc tgtggctgca tctggcggaa aatcagctgc
    30841 atctttttga tggtgaaaca ggacaacgag tatgagtaac tggccttatc cccgcatcgt
    30901 cgctcatcgt ggcggcggta agctggcccc ggaaaacacc ctggcggcaa tcgacgtcgg
    30961 ggcaaaatac ggtcataaga tgatcgaatt tgacgcgaag ttatcgaaag atggcgagat
    31021 cttcctgctc catgacgaca atctcgaacg caccagcaac ggctggggcg tcgcgggtga
    31081 actgaactgg caggatttac tgcgcgtgga tgcgggcagt tggtacagca aagcgtttaa
    31141 aggtgagccg ctgccgttgc tttcgcaggt ggcggaacgc tgtcgcgaac acgggatgat
    31201 ggcgaatatc gaaatcaaac ccaccactgg cactggacca ttaacgggca aaatggtggc
    31261 gctggcggca cgccaactgt gggccggtat gacgccgccg ctgctgtcat cgtttgagat
    31321 tgatgcttta gaagctgcac aacaggcggc accggaactg ccgcgtggtt tattgcttga
    31381 tgagtggcgc gacgactggc gcgaactgac cgcgcggctg ggctgcgtct ctattcatct
    31441 caatcataag ttactcgata aagcgcgagt gatgcagttg aaagacgccg gattacggat
    31501 tctggtttat accgtcaata aaccccagcg cgcggcagag ttgctgcgct ggggcgtgga
    31561 ttgcatctgt accgatgcga ttgacgtgat tggtccgaac tttacggccc aatagttttc
    31621 aacggaatgt caggctgcgg cgtgctgggc tgattcagca tgtcgccgtt tctcctctcc
    31681 ggcagcatat gctgctgatt caacgaacta tccggattac ggttgctgtt taacatcccg
    31741 ccgttggtgt tgggcagcat ttgctgccgg gcgggatttc gctcccccgg ctgtgactgc
    31801 aacacccgct gagaattatt gtttatctgg ttttctaaat gctgctgttg caactgagtt
    31861 tgcgttttca gttgctgatt cagcatccct ttttgctgga tttgctgagt ctgcatctgg
    31921 gtttgcatcc gctgctggct gggtatctga taccccggct ggttagggtt gttcagagta
    31981 ttaatgggct gtgcaaagcc gacaaacggc aggagtgccg taagaatcag aagtcgtttc
    32041 atcgcgtatc ctcctctgaa gatatccttt aagtttactc gcttcccgac aaaacgatga
    32101 ttaattcaga gttatatacc aggcttagct ggggttgccc cttaatctct ggagaataac
    32161 gatgataaaa ccgacgtttt tacgccgggt ggccattgct gctctgctct caggaagttg
    32221 ttttagcgcc gccgccgcgc ctcctgcggc gcccgtctcg tatggtgtgg aggaagatgt
    32281 cttccacccg gtacgcgcga aacagggaat ggtagcgtct gtggacgcca ctgccactca
    32341 ggtgggggtg gatatcctca aggagggcgg gaatgccgtt gatgccgccg tggcggtggg
    32401 ctacgcgctg gcggtaacgc atccgcaggc agggaatctg ggcggtggtg gttttatgtt
    32461 aatccgctcg aaaaatggca ataccacggc tatcgatttc cgcgaaatgg cacccgccaa
    32521 agcgacccgc gatatgttcc tcgatgatca gggcaacccg gacagcaaaa aatcactcac
    32581 ttcgcatctg gcttccggca caccgggtac ggtagcaggt ttctcgctgg cgctggataa
    32641 atacggcacc atgccgctga acaaagtcgt gcagcccgcg tttaaactgg cacgcgatgg
    32701 ttttatcgtt aacgacgcgc tggctgacga tctcaaaacc tacggtagcg aagtgttgcc
    32761 gaatcacgaa aacagtaaag ctatcttctg gaaagagggc gagccgctga aaaagggcga
    32821 cacgctggtg caggcgaacc tggcaaagag cctggagatg attgctgaaa acggcccgga
    32881 cgaattctat aaaggcacga ttgcggaaca gatcgcccag gagatgcaga aaaacggtgg
    32941 cttgatcact aaagaagatt tagcggccta taaagcggtc gaacgcactc cgataagcgg
    33001 cgattatcgc gggtatcagg tttactccat gccaccgcca tcctccggcg ggatccatat
    33061 cgtacaaatc ctcaatattc tggaaaactt cgatatgaag aaatacggct ttggcagcgc
    33121 cgatgcgatg caaatcatgg cagaagcgga gaaatacgcc tacgccgacc gctcggaata
    33181 tcttggcgac ccggattttg tcaaagtccc gtggcaggca ctgaccaata aagcctatgc
    33241 caaatccatt gccgatcaaa ttgatatcaa caaagcgaag ccgtccagtg agattcgccc
    33301 tggcaagctt gcgccttatg agagtaatca aactacccat tactctgtag tggataaaga
    33361 cggtaacgcg gtggcggtga cctatacgct gaacaccacc ttcggtacgg gtattgtcgc
    33421 gggcgagagc ggtattctgc ttaataacca gatggatgat ttctccgcca aaccgggcgt
    33481 accgaacgtt tacgggctgg tgggcggtga tgccaacgcc gtcgggccga acaaacgccc
    33541 gctgtcgtcg atgtcgccga ccattgtggt gaaagacggt aaaacctggc tggttaccgg
    33601 tagcccaggc ggtagccgga ttatcaccac ggtgctgcaa atggtggtga atagcatcga
    33661 ttatggcatg aacgtcgccg aagcgaccaa tgcgccgcgt ttccaccatc agtggttgcc
    33721 ggacgagctg cgtgtcgaaa aagggtttag cccggatacg ctcaagctgc tggaagcaaa
    33781 aggtcagaaa gtggcgctga aagaggcgat gggcagcaca caaagcatta tggttgggcc
    33841 ggacggtgag ttgtacggcg catccgaccc gcgctcggtg gatgatttaa cggcggggta
    33901 ttaatatctt aagccctccc ggtaggtgag ggcttaaatg acaaacttag attgcaaatt
    33961 ctctatttca cccgcgccat ataatacgca tcgacatatt caccattacg caatgcgtac
    34021 ttcttaccgg tcccttcaat ctcaaagccg aattttttat agaccttaat tgctggcgcg
    34081 ttatcgacaa acacggttag ttcaatgcga tctacccgta accagttgtc gcacatttca
    34141 atcatctctc gcatcagggc gctggcgacg ccgcggttct
//
LOCUS       DADLSA010000031        33649 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.031, whole genome shotgun sequence.
ACCESSION   DADLSA010000031 DADLSA010000000
VERSION     DADLSA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.031"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            1327..2106
                     /gene="ipaJ"
                     /locus_tag="JN691_001470"
     CDS             1327..2106
                     /gene="ipaJ"
                     /locus_tag="JN691_001470"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HAY7242947.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3052..3981)
                     /locus_tag="JN691_001471"
     CDS             complement(3052..3981)
                     /locus_tag="JN691_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HAY7242948.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4198..4434)
                     /locus_tag="JN691_001472"
     CDS             complement(4198..4434)
                     /locus_tag="JN691_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7242949.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4438..6339)
                     /gene="ipaA"
                     /locus_tag="JN691_001473"
     CDS             complement(4438..6339)
                     /gene="ipaA"
                     /locus_tag="JN691_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HAY7242950.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSLGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQPDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6348..7346)
                     /gene="ipaD"
                     /locus_tag="JN691_001474"
     CDS             complement(6348..7346)
                     /gene="ipaD"
                     /locus_tag="JN691_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HAY7242951.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDTLSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKNALEELKKKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSEKNGGYVVNINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7397..8488)
                     /gene="ipaC"
                     /locus_tag="JN691_001475"
     CDS             complement(7397..8488)
                     /gene="ipaC"
                     /locus_tag="JN691_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HAY7242952.1"
                     /translation="MEIQNTKSTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIAHLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMDTSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSKNSTASQIAGNIRA"
     gene            complement(8508..10250)
                     /gene="ipaB"
                     /locus_tag="JN691_001476"
     CDS             complement(8508..10250)
                     /gene="ipaB"
                     /locus_tag="JN691_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HAY7242953.1"
                     /translation="MHNVSTTTTGLSLAKILTSTELGDNTIQAANDAANKLFSLTIAD
                     LTANKNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELAPDSPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KSFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10256..10723)
                     /gene="ipgC"
                     /locus_tag="JN691_001477"
     CDS             complement(10256..10723)
                     /gene="ipgC"
                     /locus_tag="JN691_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocator chaperone
                     IpgC"
                     /protein_id="HAY7242954.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10781..11407)
                     /gene="ipgB"
                     /locus_tag="JN691_001478"
     CDS             complement(10781..11407)
                     /gene="ipgB"
                     /locus_tag="JN691_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgB"
                     /protein_id="HAY7242955.1"
                     /translation="MQILNKILPQVEFAIPRPSFNSLSYNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDNVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11423..11812)
                     /gene="ipgA"
                     /locus_tag="JN691_001479"
     CDS             complement(11423..11812)
                     /gene="ipgA"
                     /locus_tag="JN691_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HAY7242956.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11825..13309)
                     /gene="icsB"
                     /locus_tag="JN691_001480"
     CDS             complement(11825..13309)
                     /gene="icsB"
                     /locus_tag="JN691_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HAY7242957.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEE
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13623..15239
                     /locus_tag="JN691_001481"
     CDS             13623..15239
                     /locus_tag="JN691_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HAY7242958.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGNPKGVSVISYQRVKNGER
                     NKGVEALNRLYLQNQTSLTGKGLLFARDRAAVFYEAIKLAGGDTSKIKAMMEQLDTYK
                     LGEVDKRNINELNKVISEEIRAQLGIKNKKELQSEIKQIFTKYLNNKNWEPINKNINY
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESQHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELASAALYSRRELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVKALKGLNSKRGEPTKLLIRNSDGLLQEVNVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNGESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKDIINKKLQKNDNGEPYKLSQRMALLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHDTGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYAERIGDPKIWNMVKGYSSFV"
     gene            15249..15611
                     /gene="ipgE"
                     /locus_tag="JN691_001482"
     CDS             15249..15611
                     /gene="ipgE"
                     /locus_tag="JN691_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HAY7242959.1"
                     /translation="MEDLADVICRALGIPSIDTDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGSLIARLDLTGINEFEDIYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15611..16069
                     /gene="ipgF"
                     /locus_tag="JN691_001483"
     CDS             15611..16069
                     /gene="ipgF"
                     /locus_tag="JN691_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgF"
                     /protein_id="HAY7242960.1"
                     /translation="MSRFVFILLFFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESR
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYTEEMLISHPCLSVHYAAKLLNEFM
                     LMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYKRYLRIAAESKQNNRRI"
     gene            16078..17193
                     /gene="mxiG"
                     /locus_tag="JN691_001484"
     CDS             16078..17193
                     /gene="mxiG"
                     /locus_tag="JN691_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HAY7242961.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLAISKDNICQFSDEKGEQIDINSQFNSFEYDGVS
                     FQLKNMMEDKSRVHILNGMYNKPSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     YINLYVLNLVSDKAELLVFLSKERNSSKDTELEKLKNALIVEFPYIKNIKFNYLSDHN
                     AREDAKGIFTKVNVQYKEICEANKVTYSVREELTDEKLELINRLISDHKNVYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17201..17452
                     /gene="mxiH"
                     /locus_tag="JN691_001485"
     CDS             17201..17452
                     /gene="mxiH"
                     /locus_tag="JN691_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex protein
                     MxiH"
                     /protein_id="HAY7242962.1"
                     /translation="MSVTVPDKDWTLSSLSETFDDGTQTLQGELTLALDALAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17465..17758
                     /gene="mxiI"
                     /locus_tag="JN691_001486"
     CDS             17465..17758
                     /gene="mxiI"
                     /locus_tag="JN691_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HAY7242963.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVGNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17764..18489
                     /locus_tag="JN691_001487"
     CDS             17764..18489
                     /locus_tag="JN691_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HAY7242964.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEIKSEFLTNEVIYVFLG
                     MAVLVVTLLVWAFKTGWFKRNKI"
     gene            18486..19013
                     /locus_tag="JN691_001488"
     CDS             18486..19013
                     /locus_tag="JN691_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HAY7242965.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18955..19650
                     /locus_tag="JN691_001489"
     CDS             18955..19650
                     /locus_tag="JN691_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxiN"
                     /protein_id="HAY7242966.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIINTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19643..20050
                     /locus_tag="JN691_001490"
     CDS             19643..20050
                     /locus_tag="JN691_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa secretion machinery protein MxiL"
                     /protein_id="HAY7242967.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFDAGREADDSTW"
     gene            20034..20462
                     /gene="mxiM"
                     /locus_tag="JN691_001491"
     CDS             20034..20462
                     /gene="mxiM"
                     /locus_tag="JN691_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HAY7242968.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20592..21224
                     /gene="mxiE"
                     /locus_tag="JN691_001492"
     CDS             20592..21224
                     /gene="mxiE"
                     /locus_tag="JN691_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional activator MxiE"
                     /protein_id="HAY7242969.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21211..22911
                     /gene="mxiD"
                     /locus_tag="JN691_001493"
     CDS             21211..22911
                     /gene="mxiD"
                     /locus_tag="JN691_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HAY7242970.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAKNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22930..23997
                     /gene="mxiC"
                     /locus_tag="JN691_001494"
     CDS             22930..23997
                     /gene="mxiC"
                     /locus_tag="JN691_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HAY7242971.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDQDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIDVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24010..26070
                     /gene="mxiA"
                     /locus_tag="JN691_001495"
                     /gene_synonym="virH"
     CDS             24010..26070
                     /gene="mxiA"
                     /locus_tag="JN691_001495"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiA"
                     /protein_id="HAY7242972.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKISANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26083..26484
                     /gene="spaK"
                     /locus_tag="JN691_001496"
     CDS             26083..26484
                     /gene="spaK"
                     /locus_tag="JN691_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone SpaK"
                     /protein_id="HAY7242973.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26488..27780
                     /locus_tag="JN691_001497"
     CDS             26488..27780
                     /locus_tag="JN691_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HAY7242974.1"
                     /translation="MSYIKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IIARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGVKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27873..28211
                     /gene="spaM"
                     /locus_tag="JN691_001498"
     CDS             27873..28211
                     /gene="spaM"
                     /locus_tag="JN691_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface presentation of antigens protein SpaM"
                     /protein_id="HAY7242975.1"
                     /translation="MEALDKRIIYFQQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28198..29076
                     /gene="spa32"
                     /locus_tag="JN691_001499"
     CDS             28198..29076
                     /gene="spa32"
                     /locus_tag="JN691_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HAY7242976.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29070..29951
                     /gene="spaO"
                     /locus_tag="JN691_001500"
     CDS             29070..29951
                     /gene="spaO"
                     /locus_tag="JN691_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SpaO"
                     /protein_id="HAY7242977.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESDHTEVSLAL
                     FNYDDINVKVDFILLEKKMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29956..30606
                     /gene="spaP"
                     /locus_tag="JN691_001501"
     CDS             29956..30606
                     /gene="spaP"
                     /locus_tag="JN691_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaP"
                     /protein_id="HAY7242978.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLIEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FIVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            30621..30881
                     /gene="spaQ"
                     /locus_tag="JN691_001502"
     CDS             30621..30881
                     /gene="spaQ"
                     /locus_tag="JN691_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HAY7242979.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLTIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30881..31651
                     /gene="spaR"
                     /locus_tag="JN691_001503"
     CDS             30881..31651
                     /gene="spaR"
                     /locus_tag="JN691_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaR"
                     /protein_id="HAY7242980.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31658..32686
                     /gene="spaS"
                     /locus_tag="JN691_001504"
     CDS             31658..32686
                     /gene="spaS"
                     /locus_tag="JN691_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein SpaS"
                     /protein_id="HAY7242981.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32699..32941
                     /locus_tag="JN691_001505"
     CDS             32699..32941
                     /locus_tag="JN691_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7242982.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
     gene            33451..33606
                     /locus_tag="JN691_001506"
                     /pseudo
     CDS             33451..33606
                     /locus_tag="JN691_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT        10510 a         4886 c         6740 g        11513 t
ORIGIN      
        1 ttaccccctg gatctgattt caggcgttgg gtgtggatca ctattgcacc gttcgtgaca
       61 cttgttgact gcctgtgcgg catctggtat caaggtggcg tgaagccggt tcaggttctc
      121 atgcggaacg ttggaaccta tatccccccg ataccaaagg cgaaatgtaa gtgacaaaat
      181 gcaatgaaca cgttctccgt taactgagga gcatggtgat aaataaaggt aaatgcagaa
      241 ggtgtagagt ctcgcagatt tctgacatca tagagcctaa cattagatgc ataacgctac
      301 agattgtaga atctctacag gtgaaatata cactggttgg cgtttatgtc gcaaaacatg
      361 tcattcaggt tcatctcacc aataaggata tgagtgaagt ggaggataag tgagtctgct
      421 gtcagagttt tctggtgtat gtcagtaact gcgaaccatg tctgcttgat atgggagctt
      481 atggaggctc tctgtacaag gttagggagc taacaaagca gggataaaaa gtccgcctga
      541 tgcaggcacg gttcgttaaa tcttttgcca ttggtaacaa aatggtgcga tgaatgttca
      601 ggctatcttt atcttgatgg tggttcagct gtttggtaaa gaaatcgctg taacaataga
      661 agaactgcag gcagtactgg tcccacatcg tttgctcagt caactggtga agttccgtag
      721 cgcgtaaatt aatgctttgc atgaaacgtt actggagtgg atgaaagaat acctgcatat
      781 atcattactg cgttggatta ccagtacaac agattgaatg aactggacat gttgatagac
      841 ggcattgaaa agcagcttat aagcatagtg cggcagagtt aattggagag cacttcgcct
      901 tctgagtatt aagtaagcat ggtggtacct gcggatacta tctattcact gtgctcggtc
      961 ggcagcctta ctgatcaagg agttggggga gttgataaca aagttgaaaa aacagctctc
     1021 aaccaaggtg gttattgtaa caatagcaaa taagctggtg tgacctgtat cacaataacc
     1081 gattatggac gtaaatacga caaaaatatg tcagtatcag gcggtattaa ttgctgatgc
     1141 tgatggacat taactcttaa taaaagggtg gatgctgaaa ggttgttatg gtgacggacg
     1201 tgatgacgac ggaaaagagt atgaggtttt ggatttaaag ttcgttcggg gaataaggac
     1261 ccggtcagct aattctcata gtagttcgca ttgctggatg caatactgtg ctgcaattac
     1321 tcgtcgatgt cggaacaacg gaagccttgc aaacggggat gtattcatac aggtgttatg
     1381 ttatatgggg ttttattgca gggggctata ccaagagaat atatgatatc tcatcagact
     1441 gatgtgaggg taaatgagaa tagagtaaac gaacaaggtt gttttttggc gaggaagcag
     1501 atgtatgaca attcttgtgg agctgccagt ttactctgtg ctgcaaagga attaggggtt
     1561 gataaaatac cacaatacaa agggtctatg tccgaaatga caaggaagag ttctcttgat
     1621 ttggataatc gttgtgagcg ggatttatac ttaataacta gtggtaatta taatccgaga
     1681 atacacaaag ataatatcgc ggatgccggt tattctatgc ctgataagat agtcatggct
     1741 acgaggcttc ttggattgaa tgcttatgtc gttgaagaaa gtaacatttt ttcgcaagta
     1801 attagtttta tttatcccga tgcaagggac ttattaattg gcatgggatg taatatagta
     1861 catcaacgag atgtgttatc ttctaaccaa agagttttgg aagctgttgc tgtatccttt
     1921 attggagttc ctgtcggcct ccactgggtg ttgtgtagac ctgatggaag ctatatggat
     1981 ccagctgtag gagaaaatta ttcttgtttt tcaactatgg aattaggtgc aagaagaagt
     2041 aattctaact ttataggata tactaaaata ggtatttcca tagttataac taatgaggct
     2101 ttgtaaattg tcgtttttta tctgtgaagg agcttgaata ttttggggcg gggggcataa
     2161 caaatgatat tactgagtac aaatttagct atattgggcg gattagttgt gtgggaagac
     2221 gaaatgtttc tcttgaaata agtgaactcc ctcaaggaat gattgtgtgc ttaaatattt
     2281 aagtgctggt aagtttgaga aggctgacat attttgcaga gcaaacagtg attgacttgg
     2341 atgatatttt tattttagtc actcgttgta attggagtat gctttatttt atttgaaaat
     2401 agatgtattt tatacccgca atttaaactg taagccctat gaggggtatg gatattataa
     2461 gttactcttt gactaagcat aacttttggg gttagaaaaa taaatctctt gttaataatt
     2521 aatatgattt taaaatcaaa cttgtttata attataaaat aatgtaagtc ggtcatttta
     2581 tgggaagatt aggagaataa aacagtaatt atatttgaga aatctgctaa tatattctta
     2641 aagctttttt gatactgtga acgaagtctg ttaatggtgg attttttgtt tatcaccatg
     2701 taaaaaaatt gagtagtaaa agcgttgact gctaatcctc gctgttttta ttgaaacgat
     2761 taaaaatacg ctgaatattt tcggccagtc actcatcttc acataaaatt ttgaccatca
     2821 tgccgcatcc cttagttgca caattcattt tcaatgatat gaatattact aatttcacca
     2881 gataatgttc tcgtggtcag cagggtatga gttttcaccg taattttaag gaaacacagt
     2941 gcgtggggat tgaataacag atagccgacg gaattacccc accggctgaa agtcaagttc
     3001 aacgaagatt tttattatct gaattgggca gtttacatca gtgttcgatg tttatgaaga
     3061 cgatagatgg cgagaaatta tatcccgaat agcttcatct atttctctct gaactgtttt
     3121 gtttatttta gataatgtaa atattagagt tctgtttgtt ttattctctt ttctttttat
     3181 gtaagtccgt ttgtctttag aaataaataa cgagtcaact tttagcgaag ggttttgctt
     3241 tctgtttttg cttttcttta tgatgtttaa aatttctttt ttatatatgt ccgggggcaa
     3301 atttgtatca aggtctttta tttcttcctt taatattggt agtgtagaac taagagattc
     3361 attagccttt tcaagtcctt tataataatt gaataatatc ttgtagtcat taaagttaag
     3421 ttctgacgcg attggaaata gagatattat ttcctgtgga acgcttgctg cctgaaaggc
     3481 acgagtgact ttcgcgcgag acagattctc ttttttggct atgtctttat aggacatccc
     3541 tgatactttc agaaaattaa gaccaatacc aagttctcgg atgctatgct cttttgctgt
     3601 ttgtatatcg tttgctagtt ttctggcatc aagagtagat atatattctt ttgaatatag
     3661 aacttcaaga tctgctcctg catatattgc agatgctctt ctacgagtgc catccagaat
     3721 ttcaattcta ccatcaatct cccttcctat tacagggaag aattgttgta gctttatagt
     3781 ttttatgata tcggctaatg attcttctgt tagggattct tgatcgcgta cattcgtttt
     3841 ttggttgacg aaggttaaat cttggatcct tttatgagga atcttggctt tgataaagga
     3901 aactttatta ccagaatgga gtgtaaactc tttcttttgg ccttccttta tacttaacaa
     3961 gtcgttgcac aaatccacca tatcacaccc tgtttattca tattgattta tattgacaaa
     4021 agttaaatgc agtgtgagca agattgtatg taactgttga tgatagtcaa cggtagtctt
     4081 aaaaattact attttaatag ctggattgat gcaatttatc tttattggat aaaagaataa
     4141 tattcttgtg gagctctgat gtgagagctc catagatgaa aaaatgctta tcaattacta
     4201 tattgatttt gtactatttt tgactacaga aataagatct ccaatagtaa taatgtgttc
     4261 taatgcagat tcatctatac gtagattgaa ctcaaaacta atcatatcca gcatttcaat
     4321 cagagaaaga gaatcggcag aaagatcttc aataaattta gtttcttcag atagtttgct
     4381 gtacgctata ccatagcaaa aggccactat tgataatatt ttttctttta tcatatttta
     4441 atccttattg atattcttta atacttttga tagggaagtg gttacatctt ttgctgcttt
     4501 atatatatta ttgttttcag ctgtaatatc attgttattt tttaaatcag atatatcatc
     4561 tgtaagtaat tctgcagagg tatcgtcaat ttttgacaat accttgctga gggcgctaga
     4621 tacttctttt gccttttcat agatcgcatg atttttatca attgtatctg cgttggtttc
     4681 tgttacgtag tttattgtat ttgcatctgt aacttttaat ttgttgttta ttctgtgttc
     4741 tgttgtggat tttgtaatgt cctttgattt ttcctgtaac tctcgttctt gtgttcctga
     4801 tttagttcca atgagagata taagattact tagggagttt gttacttttt ttgaagcttc
     4861 aaatatcgtt tcccgtgtta aatatgtgtc tccatttctt gataaaaaat caggctgaag
     4921 tacttttgaa tcagtgtttg agttgaattt attgatttgc gaagctactc tatgagaaag
     4981 agaagcgcta cttttagttg tatctgatga gttatcatta ctgcttgtca ctccttccgg
     5041 gtggagaggg ggggtggttg aagagttctg tatgttcaga ggcctgtgtc ccagagaaag
     5101 agagttattt gcagatcttt cttgactact cgtagttaac aggtctgaac ctattccatc
     5161 cgaattatct ggagtatggt tgaaaacctt ttctaaagct gaattatttg caaatgtctg
     5221 gacagtatct gatatagagt gtggtagaac ggaagttact ttttgaacta gtgctctttg
     5281 tggatttagt aatgagtttc cgttacggga taatagatcg tttgtctgag aagtaggaga
     5341 ctgtgcatca ttttctgggt tctcgtgata gctattgtca tatactctgt tgtcaaaaac
     5401 tctattatcg taagttctat tatcattatt tatatgatag tgaattacag gttgggatgt
     5461 tttctcattg gctccggggg ttggtccgcc atctggcata tcttcgggag gtgtagggag
     5521 gccatcttgg ataggatgcg aatcttttat atcttctaaa atatcattaa ggacttttct
     5581 caacagctca ggaaacacgg ctactgtatt taagtctctt gacagtttac ctataccaaa
     5641 tcctttacta tcaatagtat ttttacgttg attgcgcaaa ctatcaaagg caagttttgc
     5701 ctgtgcgtca actaaatcat ctagtttgag agatgaaaca gtttcattac ttaactttaa
     5761 ttcagcaaga atattattct tgataaatga tgatacttca gtattgagga gtttttgtcc
     5821 caatgataaa gcttcaccag atgtgtgttg ttgtgctaat agtgcaagtc tgtgctcaat
     5881 gttttgtcgt aattgatgga gtttcttatt ctttgccgac catggtccaa aggcagaatc
     5941 ttgggataat ttatctttta taattagttc cgaatactgt gcgacaattt tttgaagtcc
     6001 cttttcgttt tcaaaattaa gaccattttg atgtactaag gagtacaaaa catttgaaaa
     6061 ttcgagaatc tcgcttttgg catacgcatt cattgagttg gaataaatgc ttcttaacac
     6121 atcttctttc cctgaggaag aaaataaaaa atcaagacat ttctgattaa aagacgttgc
     6181 aaagttttct ttttcagaca ctgatgctgg tgtttttaga gaagtttgcg aactatggat
     6241 atcggatatt ttgcttttta actcgctgta ttctgttgat gatggtgaag ttgccttata
     6301 taagaatgtt ggcgcttgag tattatttac attatgcatg gcgcacctca gaaatggaga
     6361 aaaagtttat ctgtatctgt acatgagctt attgtactac tcaaaacctt tactaaatta
     6421 tcaaaaatac tattggcatt actgtatttt tgaactaaag tttgaagatt atttttcatt
     6481 gtttcatctt cggcagagaa tccggcattc catgcctgat attttgcatt atctagcaca
     6541 acctcgccat ttccacctag attatctaag ctttttaaca tattgtctat tggggtcatg
     6601 tttatattga caacatatcc cccgtttttt tcagatacct tgccgattgt tccacctaat
     6661 tctgtaagcc atttatttgc ttgttcctga ctaacagtat tatttgctgg atatagcggt
     6721 ttatctttat attttttctt gagttcttcc aatgcgtttt taagcgagtt gacttgtaat
     6781 ttcacggagt ttccgtcgtt acctccggga gagatccagc cggcaagact ggaaagaaca
     6841 gcgctaaaat cttgatacat ttgagtatat gaactaacgg catgttcata tactttcaga
     6901 tactgttcat taatatcatt gatggagttt gcaattttag cccacagttc tctatgagat
     6961 atcatttgat ctccatcaag ctcggcttct ttcggggctg aatgtaataa ttctcttgcg
     7021 tctttattaa ttggatattc gttcctggaa agagtatcca atagttgagc ggatttattt
     7081 acatccatgc taatctgaga tgaatgtaat gctatttctt ctagcgatgt tttagtcaac
     7141 gttttttgtg aaagctcttt ctttaatgcc tgatttgttg ttctgatatt atgaagggtg
     7201 tcgttgagca tagtaaggga acttacagga tgagaactgg tcgttgtttt tatatcagaa
     7261 ttaactgttt cggttgatga accgttggta ttgtttggac tgaatgatga ggtggaaata
     7321 ctattagtca gagttgttat attcatggtt atttccttac aggaagagcc atatattatt
     7381 atggctcttg gatatattaa gctcgaatgt taccagcaat ctgactggct gtcgaattct
     7441 ttgattgggt gatgctgtca attatattca ataatttttg tattaattga tttgtcgctt
     7501 gggatgcttc tttagatact tgggatgctt cctctgcttg tttactgctg gcctgactga
     7561 ttagttgttc ttcttcttca agagcagatg cataacgccc tcctgatgtg gatatattac
     7621 cagcaactgc tgatgtgtcc attgttgcac ggccaatgtt ttgtttttgc tgcgcagaaa
     7681 gggtattgag ctcgtaagtt cttctctggg tgtcaatttt atcctgcaaa gaaatatcgg
     7741 gagaactaag agaagtttta tgttcagttg acagggatat attatctggc gccagtttat
     7801 ttttacctaa aaattttgtg ctagagttag tttgtggtga ggtgatattt gtatctattt
     7861 gtttatttaa ccctaattta gaacctgcaa gctctttttc aagagattga gcagtggcaa
     7921 ggttctttct taaggctcct ttttggtcgc taatccctga atgcgttttt ttggcaccga
     7981 tacccgttat acctacttgt gtgactgctc cagtaatgct tgatgatagg gctgccaggc
     8041 cttgccgaac aatgttctct gcagctgatt ttgtagcatc gaacgcaatc aatgacaatt
     8101 gagagcccaa tttagtttct gcagtgcgga gagcagaaag tagaacggct acactaataa
     8161 ttaaagaaac agcattagaa gaaagactgg aaatatccag agtgttctct ggagtaaggg
     8221 tgtgactcat ctctgaaaga tctttcatct ttttgtcagt aaggtgagca atgatttctg
     8281 aatcatgctg aacctgctct gataacttta aaagttgatc atcatttaat gtggttgtta
     8341 atacgggatt tttaccgaca ttaagtggaa tatgcgctgc aatctgctga taattttgtg
     8401 atttttgtgt ttcggaagaa ctttgagttt gttttgtaga tatatctgta tataaaatct
     8461 gggttgattt tgtgttttga atttccataa cattctcctt atttgtatca agcagtagtt
     8521 tgttgcaaaa ttgcttttgc aacatcagtt ctattagcct gagagttgga cattgaggct
     8581 aatagatctg caattacttc ctgcaattgg ccgaattttt ctattgcttc actgatatat
     8641 tttgacagtt gttcaacttg atatttcgat aatgtcaggt ctgctagatt tgtcgacgcg
     8701 ctgttctgga aaacagcaga agcgacactt cctcccgctt gtgtggcaga gttaacactt
     8761 tctcctaata aaactgcttg gtttaaatgg gtggaaataa tttgtttgga tattacttca
     8821 tcacccgctg caccaagaaa tttatttaat ctggcaacag cattagtgat taaatcgtcc
     8881 agttgagaag aaaaactctt gagaaacttt ggtataaggt ctgtgagggt tttacctatt
     8941 attttgccaa tattctctgc aagttttgct gctgcctgtt taccaacagt ggctacgaga
     9001 acgactgctg caactaggac aagagcgcct gcgattgccc ccagaataga gccaatcatt
     9061 ttggcttttt tcgagtcgac gcccaagcct tcgagcattt ttgtaaatgc atctgaaagg
     9121 agtttgatta agggttcaat gactgctttc atgatcggat tcagggcttg ttccatgaag
     9181 gaattgccgg tcgctgcttg tactatagca tccgtaacca taagagctaa accaacagct
     9241 gccagtgcta gagaggctcc tccagaaaaa gctgctgcaa caacactaac gatagttaaa
     9301 agtgccccaa gtattttccc aacacaaccc attactctgt tgagttcttc tgctttacgt
     9361 acttcagcag catactcatc agattttctc tccatttcag tttttcttga ttcttggaga
     9421 gactggaata gagccagatc attttttaaa gattcttcat tattttttcc aactagttga
     9481 ataaaggttg ccatcaattg agtaacactg gcaagtccgg ttaatgattt ctgctgggtt
     9541 gatagctgtt cagcagatgc tgtgtttgaa aatgcagaaa aagagtctat ttctttttcg
     9601 agttgcattg atttatctgt aagtttgcta tgaattgaca gggttttctg ctcaatcaat
     9661 gtcctgtctt taactgctgc gtcttttttg atagtgagtt gtatttcttc ccggcttaat
     9721 tttttctttt ctggtgaatc tggggcgagt tcggataatc ttgtttgaat ttggttaatt
     9781 ttattttcta ggtcttttat tttagaatct gcgtttttta gtttattaat ttgtttttca
     9841 tagtctctgg ttagtccttc agtttcagat agaagagtat taattttatc ggagaattct
     9901 aggttttttt gctgtcttgc ctgttgctga gacttccaag cagtaatttt atttgttaat
     9961 gcagttaaag atttttcacc gagtatttga ataaggtttc caattaaaag cgttagttgg
    10021 gaacttgcat ttaatgactt tggtgcttta agttcaggga ttaatatatt tgaagttgag
    10081 tgtgcattag ttgtattaat atttttgtta gcagtaagat cagcaattgt aagagaaaat
    10141 aatttgttag ctgcatcatt tgcagcctgg atagtattgt ctccaagctc agtggaagtc
    10201 aatattttgg caagagacaa accagtggtt gtggtactta cattatgcat aataattact
    10261 ccttgatatc ctgaattgcg tccaagtatg attgtgcttt tatttttaat ttttcatcat
    10321 tgctgtgttg aattacgagt tcgaagcact ctttggcttt caagggggct ttcaaccgaa
    10381 gctggcattg tccagtatgg aataccggtg tatagtcatt ttttcctaat gcaaaagcga
    10441 cagcataaag gtctgctgct tgttggaact gttcttttat ctgataaata gctgcgagtc
    10501 ccataatgta gtctacattg taaaagtcgt atatacataa aaatctgaag aaaacttcag
    10561 cttcctctat tcttcctttg ttgtaaaagt cataagcata tgaataaatg tcatccatca
    10621 tatcatcagg aattgcatta atatctttca gtgtagcgcc agagttaatt gcatcaatta
    10681 ctgcagtaga gatgctttca ttttcagtga tatttaaaga cataaggtct cctttttgtc
    10741 tgctttactt taaaaagtat aagatttaat ataaacagat ttaatttgta ttgctttgac
    10801 ggtatacggc ctttagaacc tctgcgctta tttctatttg taaaactttt gttgattcat
    10861 taaatatttt atcgtagacg gaactggata ctagatttgt aagatccttc atttgtgtgt
    10921 taaacaaatc catgtcactg ctatagcgtt ttaaaatctt tgctctgagg ccagatgagg
    10981 caatctcgtt tagtgctgta tgactgatag atgtttgtgc tgatttttca tcaagtttta
    11041 tatccgggaa ttttttttca atagctgcaa atatgttttt tttgttatct gaggtcaaaa
    11101 caactccatt gtgtatggcc atttcatcta tacttctgtt tatcattgaa gagacataag
    11161 aggttcgctc ttggctcatc caacaaaata gttgattccc cgaattacta tcttttattt
    11221 tgtctatgac gttgtccctg attttattta tattcaccgg gcatccaaaa ttcttctgag
    11281 gaaagcgttg ttttaagtta aaaacagata atattttctt tacaagctta ttgtaactta
    11341 acgaattaaa tgatgggcga ggaatcgcga attcaacctg tggaagtatt ttgtttagaa
    11401 tttgcatgat accccctata tgttagttca cttctgaagt gatgtttgca atggttagtt
    11461 taatacaaaa taaaaattca tacaaagatt caatgagttc atcaggtgaa agatattttt
    11521 tgtagtgttt cttggtcaaa acacattgat gagtatcagg gagaagacag atagaagaac
    11581 cataatctct actcaaagag tagttaagga tgagtatgtg ttctggatta ataatatttt
    11641 ctgtaaaaag ttcatgttca ttaagaagtc cggtcaaaaa gatgtattca tcattatctg
    11701 ttatacaaac aggtatttgt tcttcgagca atataaaggc ttgattttta tcgttaaaat
    11761 caagctttgc acctgttatt tcataaagtt tatcatatag tttgcgacac atttctatgg
    11821 ccttctatat attagaatga gagttattca ataaaagctt attgatctgt aataagtcgt
    11881 gaatgtaaag tttggttcca agatctggcg atttaagaga attgtaataa tcaatagttt
    11941 ttattgactt ttcgataatt tcttcttttt tatctgttgc tccggttttt aattcgttta
    12001 atacacgaga catattgctg ggtgctgatt ttactaagta tgagtggcaa gcgttgatga
    12061 ccgcatcaaa gtcttctgaa gatacatttt cagtaggatg tttgtaaagt gaaattgata
    12121 aaggcttgaa tgtttttggg gtgtggataa ggttaatttt attgaaactt ttaagatagc
    12181 tacgtcgtaa ttgagtcaat ctggaaagat caaaatcaga ttcaatatac ttcattatct
    12241 cgttataaga ctgattgaga ctttcaatgc gttgcctaac tttatttgca tacgcgtata
    12301 cattgttagg tgtagcaacc aatttaacgt cagggagtgg aaagtatatt tcagaatttc
    12361 ctgctttaag aacattaagt gccataccag cacagttttc agatttactg attaatttat
    12421 aatttgccaa ttgtctgaaa gcatctgctt tcttttggca tataaatttt gacatgtttg
    12481 tttcatcaag gccaaaaagg ttatagctga ttttaccatc ctttgtttta ttatcacctg
    12541 ataatggaat gtatacttta tcagcactga ccccccaata accatagggg tctttttttt
    12601 gattagctct gggaaaaaat atttttttgt tgttattttg atcttgtttt gaatttaata
    12661 agtttctaac ctcttggcct gcatcaagtc tttcggctgt ttggtctgaa atcatattta
    12721 gtttgtcttg tttataggta tcgacactgt aacctgagcg tttttcaaaa aggtagttta
    12781 tttctgtatc ttttgtaaca ttcccgccgg gataccagct gagataggta tgagttggtt
    12841 cctgtagtaa attgtttttt atactgatac tggtatgccc taaatgggtt atgcctctat
    12901 ttccccagca acgaaaaact aaatgtcttt caagattttt ggctgtaccg atgccatgaa
    12961 aacgctctgc ggtattcaac attaatttga tgtctttacc tgtaaaagaa tgttgaattg
    13021 aatttttgac tgcttcatag gcggcaaacg cactttctgc agatttttcg ctagctaatg
    13081 tcttgaggaa aagcaataag gatttttgat tcgctagtcg ataatttttt agttgttcat
    13141 ttaatatctg tgaatttatc tttgcattaa tagtgcttaa tgttctgaaa aagagatcct
    13201 ttaggttgaa tttctgtgct accaatatag gtccatgctc tgtatcttca acgcgtatag
    13261 gcccttttgt attgcttgcg tcaatgaaat tgctaatttt gagaatcata ctttattaac
    13321 tctccattac gtggtgattg tggggatgta aaatgtattt gatatataat atattcaatt
    13381 aataaattta gaaacttgag cctgttaaca taatcaaatt ttcttttgtt gtacataata
    13441 tgtacctcgt gagcatatgt agtgctcgtt tcatcatgaa atcccacaag ttaaagtgtc
    13501 tgatatatca ggctcggagt gttatagaaa aagagagaac cctgttgaat aagtatcttt
    13561 tttgttgtaa aacgaggtac aaaactgcaa tcaatgcccc atttatgacg gaggataatt
    13621 aaatgcacat aactaatttg ggattgcatc aggtttcatt tcaaagcgga gattcctata
    13681 aaggcgcaga agaaaccgga aacccaaaag gtgtaagcgt gatttcatat caaagagtta
    13741 aaaatggaga gagaaataag ggggtcgaag cgcttaatcg attatattta cagaaccaaa
    13801 caagcttaac agggaaaggc cttttgtttg cgcgtgacag agcagccgtt ttttacgagg
    13861 caataaagtt ggcaggtggt gatacgtcaa aaattaaagc catgatggaa caattagata
    13921 cctataaact tggtgaagtt gataaaagaa atattaatga gcttaataaa gtaataagtg
    13981 aagagataag agcacagcta ggcattaaaa ataaaaagga attgcaatcg gaaataaaac
    14041 aaatatttac taaatattta aataataaga attgggaacc gatcaacaaa aatattaatt
    14101 atcatgggaa aaattatggt tttcaattaa cacccgcctc tcatatgaag atagggaata
    14161 aaaatatatt tgtcaaagag tataatggaa aaggaatttg ttgtgcttct acgagagaga
    14221 gtcagcatat tgcgaatatg tggctttcaa aagtggtgga tgacgagggg aaggaaattt
    14281 tttcgggtat cagacatgga gttatatcag cctatggatt gaaaaaaaac tcatctgaaa
    14341 gagctgttgc tgctcgtaat aaagctgagg agttagcaag cgcagcatta tatagcagac
    14401 gagaattatt atcacaggct ttgtctggta aaacagtaga tttaaagatt gtttcgactt
    14461 ctcttctgac gccgaccagt ttaactgggg gagaggagag tatgttaaaa gatcaggtaa
    14521 aggcattaaa agggttaaac tctaaaaggg gggaaccaac gaaattatta attcggaata
    14581 gtgatggtct tctgcaagaa gtaaacgtta atctaaaagt ggtgacattt aactttggtg
    14641 taaatgaatt agcgctaaaa atgggcttag gctggaggaa tgttgacaag cttaatggtg
    14701 aatcaatatg ttctttgttg ggggataatt tcctcaaaaa tggtgtgatt ggaggttggg
    14761 ctgcagaagc tatagaaaaa aatccaccat gtaagaatga tgtgatatat ctggctaacc
    14821 agataaaaga tattataaac aagaagttac aaaaaaatga taatggagag ccgtataaat
    14881 tgtcacaaag aatggctcta ttggcttata ctataggtgc tgtaccttgc tggaattgta
    14941 agagtgggaa ggacagaaca ggtatgcagg atgctgaaat taaaagagaa ataataagaa
    15001 aacatgatac agggcagttt tctcaattaa atagtaaatt atcctcagaa gagaaaagat
    15061 tattttctac tattctaatg aatagtggta atatggaaat ccaagagatg aatacgggtg
    15121 tgcctggaaa taaagttatg aaaaaattac cgctatcttc gttagagctg tcttatgctg
    15181 aaagaatagg ggaccccaaa atatggaata tggtgaaagg gtattcgtca tttgtataag
    15241 aggaatatat ggaagattta gcagatgtta tttgccgagc cttgggtatc ccttcaattg
    15301 ataccgatga tcaagcaatc atgcttgatg atgatgtgtt aatatatatt gaaaaagaag
    15361 gagattcaat aaatttatta tgcccttttt gtgcattgcc tgaaaatata aatgatctta
    15421 tatatgcgtt gagtctaaat tactcagaaa agatatgttt agctactgat gatgaaggtg
    15481 gaagtctaat tgcgcgatta gatcttaccg ggataaatga attcgaggat atatatgtta
    15541 atacagaata ttatatttcg cgtgtgcgtt ggctgaagga tgaatttgcg cgaagaatga
    15601 aggggtatta atgtcccgtt tcgtatttat tttgctgttt tttatcccat atttagggag
    15661 agctgattgt tgggataagg ctggtgaaag gtacaacatt ccatcgagtt tgttaaaagc
    15721 gattgcggaa aaagaatcca gatttaataa atctgcagtc aatgttaata acaatggaag
    15781 taaagattat ggtataatgc aaataaatga ctttcattct aaaagactta gagaaatggg
    15841 atatactgag gaaatgctaa ttagtcatcc atgtctttct gtacattatg cagcaaagtt
    15901 attaaatgag tttatgctga tgtatggaag agggtgggag gctgttggtg cctataatgc
    15961 aggaacatct ccgaaaaaga aaaaagaaag attaaagtac gcagaggata tctataaacg
    16021 ctatttgcgg atagcagcag agagcaagca gaataatcga aggatataag aggatttatg
    16081 tctgaggcaa agaactcaaa tcttgcacca ttcaggttat tagtgaaact cacaaacggt
    16141 gtcggggatg agtttccgtt atattatggg aataacttaa ttgttctcgg acggacgata
    16201 gaaacacttg agtttggaaa cgataatttc ccagaaaata ttattccagt aacagatagt
    16261 aaatcggatg gtataattta tttggccata agcaaagata atatctgtca atttagtgat
    16321 gaaaaaggtg aacaaattga tatcaattca caatttaact catttgaata tgacggagtt
    16381 tcttttcaac ttaaaaatat gatggaggat aaatcgagag ttcatatact aaacggaatg
    16441 tataataaac cctcggtgtt ttttttcttt gctgttattg ttgtgttaat tataattttt
    16501 tccctctctt taaagaaaga tgaagtaaaa gaaattgctg aaattataga tgataagaga
    16561 tatggaatag tcaacacagg acagtgcaat tatattcttg ccgaaacaca aaatgatgcg
    16621 gtatgggcat ctgtagcctt aaataaaacc ggatttacca aatgcagata tattctggtg
    16681 tccaacaaag aaataaatag aattcaacaa tatatcaatc aacgattccc atatatcaat
    16741 ttgtatgtgt tgaatttagt gagtgataaa gcggaattac tggtattttt aagtaaagaa
    16801 agaaatagtt caaaagatac cgaacttgag aaattgaaaa atgctctgat tgttgaattt
    16861 ccgtatatca aaaatattaa attcaactat cttagcgatc ataatgcaag ggaggatgct
    16921 aaaggtattt tcacaaaggt aaatgtccag tataaagaga tttgtgaagc taataaagtt
    16981 acatattctg tcagagaaga actgacagat gaaaagcttg agctcattaa tagactgatt
    17041 tctgatcata aaaatgtata tggagatcag tatattgagt tttctgtact tttgatagat
    17101 gatgatttta aaggtaaatc atatcttaac agcaaagaca gttatgtaat gttgaatgat
    17161 aaacactggt tttttttaga taaaaataag tgaggataaa atgagtgtta cagtaccgga
    17221 taaagattgg actctgagtt cattatctga aacttttgat gatggaactc aaacattaca
    17281 aggtgaacta acattggcac tagatgcatt agctaaaaat ccttcgaatc cacagttgct
    17341 ggctgaatac caaagtaaat tatctgaata tacattatat aggaacgcgc aatctaatac
    17401 tgtgaaagtg attaaggatg ttgatgctgc aattattcaa aacttcagat aatagggagc
    17461 attcatgaat tacatttatc cagtcaatca ggttgatatt atcaaagcca gtgattttca
    17521 atctcaagag atatcaagtc tggaagacgt cgtgtcggct aaatatagtg atattaagat
    17581 ggatacagat attcaagtat cacaaataat ggagatggta ggcaatccag aatcattaaa
    17641 tccagaatct ttggccaagt tacagacgac gctctcaaat tattcaatag gagtatcatt
    17701 agctggcacg ttagcaagaa aaacagtttc ggctgttgaa actttattaa agtcttaatt
    17761 tatatgatta ggtataaagg ttttatttta ttcttgttgc tgatgttgat tggatgtgag
    17821 caacgtgaag agttaatttc taatttatct caaagacagg caaatgaaat aatatctgtg
    17881 ctagaacgcc ataatattac tgctagaaaa gttgatggag gtaaacaggg gatctcggta
    17941 caagtcgaaa aggggacatt tgcatcggca gttgatttga tgcgcatgta cgatttgcca
    18001 aatccggaga gagttgatat ctcacaaatg tttcctacag attcattagt gtcttctcca
    18061 agagctgaaa aggcccgttt atatagtgct attgagcaac ggctggaaca gtctttagtt
    18121 tctattggtg gtgttatttc ggcaaaaata catgttagct atgatcttga agaaaaaaat
    18181 atatcttcaa aaccgatgca tatatcagta atcgctatat atgactcacc gaaagagtct
    18241 gaactattag ttagtaatat taagcgattt ttgaaaaaca ccttttctga tgttaagtat
    18301 gaaaatatat ctgtcatatt aactccgaaa gaagaatatg tttatacaaa tgtacaacct
    18361 gttaaggaaa ttaaatcgga atttttaaca aatgaagtaa tatatgtatt tctcgggatg
    18421 gctgtactag ttgtcactct tttggtatgg gcattcaaaa cagggtggtt caagagaaac
    18481 aaaatatgat aagaatggat ggaatttata aaaaatatct ttcaataatt tttgatccag
    18541 cgttctatat aaatagaaat cggttgaatt tgccttctga actgttagaa aatggcgtaa
    18601 tcagaagtga gattaataat ctcataatta ataaatatga tctaaattgc gatattgaac
    18661 ctttaagcgg ggtaaccgct atgtttgttg ccaactggaa tttacttcca gctgttgctt
    18721 attttatcgg tagtcaagag tctcggttaa ttaatcatag tgagatggtt attagttatt
    18781 atgggggcaa aatcagtaag caaggggagg ccgctattcg aagtggtttt tggcatctta
    18841 ttgcgtggaa ggaaaatata tctgttggta tatatgaaag aataaactta ttatttaatc
    18901 caattgcttt ggagggaaat tatacgcctg tagaaagaaa tttgtctcga ttaaatgaag
    18961 gtatgcaata tgcaaaaagg cactttaccg gtatccagac atcatgccta tgatggtgtt
    19021 gttataaaga gaattgaaaa ggaattatgt aaaaccataa aagatagaga cactgagagt
    19081 aagaagaaag caatttgtgt tataaaggat gctacgaaaa aagctgaatc actgcgtata
    19141 gatgctgtat gtgatggtta tcaaattggt atacagactg cttttgagca tattattgac
    19201 tatatttgtg aatggaagtt aaagcaaaat gaaaacagaa gaaatattga ggactatata
    19261 actagtttgc tttcagaaaa tttacatgat gagaggatca ttaacacttt attggagcag
    19321 tggcttagtt cgttgcgtaa tacagtaaca gaattaaaag tggttttgcc taagtgtaat
    19381 ttagcactga ggaaaaagtt agaacttgat ctccataaat atcgctcaga tgtcaagatt
    19441 attttaaaat attctgaagg gaataattat atattctgta gtggtaatca agtagtggag
    19501 tttagtcctc aggatgtaat atcaggtgtt aaaatagaac ttgcggaaaa attaacaaaa
    19561 aatgataaaa aatatttcaa agagttagca cataagaaac taagacaaat tgcagaagac
    19621 ttgttaaaag agaatcctgt taatgattaa tcaaataaat gcaagcaatg ctctgcagca
    19681 aagattaaat agtgaagaat ttgttaattt aaatgagaga ttatcatctt cgcaatcatt
    19741 tgatgaggat ataatctatg aaataatgca atatttctca cagagtgaat taaattccat
    19801 agataatgat gaactgcaca ataagattga acaattattc aattcacgtt ttccttatct
    19861 tacagctgca caaaaaagtt cacttcttaa taaactcata gatgcaaatc aatatgtcga
    19921 tttgcatgag ggattttatg catcattgtc catatataac aatattgatt tctatattaa
    19981 gactacaacc ttcgattcat taattagtgt ctttgacgca gggagagagg cagatgattc
    20041 gacatggtag taataagttg aaaatattta ttttaagtat attgctatta acactgagtg
    20101 ggtgtgcttt aaagtcatca tctaattctg aaaaagaatg gcatattgtt cctgtaagta
    20161 aggattattt ttctattcca aatgatttat tatggtcgtt taatacaacc aataaaagta
    20221 taaatgttta ctctaaatgt attagtggta aggcggttta tagttttaat gcaggtaaat
    20281 tcatgggcaa ctttaatgtt aaggaagtag atgggtgctt catggatgca caaaagatag
    20341 ctatagataa actattttct atgctgaaag acggggttgt tttaaaaggt aataagataa
    20401 atgataccat ccttatagag aaggatgggg aagttaaatt aaaattaatt cgagggatat
    20461 aattgtattg tgagtaaata taaaggtcta aatacaagta atatgtttta catttactct
    20521 agtggacatg aaccagttaa cgttgagctt gtaaaagata aagaacgtaa cataattgag
    20581 ctggctccag catggaaggg tttttttttg tccgaaatca aaatataaaa ttcagtgata
    20641 atgtgaacta tcattatcgc tttaacatta atagttgtgc aaaatttctt gctttttggg
    20701 actatttcag tggtgctcta gtagagcata gccatgctga aaaatgtatt catttctatc
    20761 atgagaatga tcttcgggat agttgtaata ccgaatcgat gcttgataag ttaatgttaa
    20821 gattcatctt ttcatctgac cagaatgtaa gtaatgcact ggctatgata cgaatgactg
    20881 agtcatatca tttggttctt tatcttctga gaactattga gaaagaaaag gaggtaagaa
    20941 taaagagtct gacagagcat tatggtgtgt cggaggctta ttttagatct ttatgtagaa
    21001 aagctttagg ggcaaaagtc aaagaacaac tgaacacttg gcggttagta aatggtttat
    21061 tagatgtatt tttgcacaat caaacaatca cttctgctgc catgaataat gggtatgcat
    21121 caacatccca tttttcaaat gagataaaaa cacgactagg cttttctgca agagaattat
    21181 cgaatattac ttttttagtg aaaaaaataa atgaaaaaat ttaatattaa atctttgact
    21241 ctcttgattg tattgttacc attgatagtt aatgctaata atattgactc tcatttatta
    21301 gaacaaaatg acatagcgaa atatgttgct caaagtgata cggtaggatc tttttttgaa
    21361 cgtttttctg cgttattaaa ttatcctatt gttgttagca aacaagctgc aaaaaagaga
    21421 atatctgggg agtttgattt atctaatcca gaagaaatgc ttgaaaaact gacattactt
    21481 gtgggactca tatggtataa agatggaaat gcattataca tatatgactc tggagaatta
    21541 ataagcaaag ttattttgct ggagaatata tcacttaatt atcttattca gtatctgaag
    21601 gatgctaacc tttatgatca cagatatcct ataagaggaa acattagtga taaaacattc
    21661 tatatatctg gtcctccggc attggttgaa ctggtagcta atacagcaac tttgcttgat
    21721 aagcaggttt cttctattgg gacagataaa gttaattttg gggttataaa attaaaaaat
    21781 acatttgtta gtgacagaac ttataatatg agaggggagg atattgtcat cccaggggtg
    21841 gctactgttg tagaacgttt attaaataat ggtaaggctc tgagtaacag acaagccaaa
    21901 aatgatccga tgccaccctt taatattaca cagaaagtat ctgaggatag taatgacttt
    21961 tcatttagtt cagttaccaa tagttctata ttagaggatg tatcacttat tgcatatcca
    22021 gaaaccaatt ctatattagt caaaggtaat gatcagcaaa tacagattat tcgcgatatt
    22081 atcactcagt tagatgtagc caaacgacat atagaattat cattatggat aatcgatatt
    22141 gataaatctg aattaaataa ccttggcgtt aattggcaag gaacggcttc atttggtgat
    22201 tcgtttggtg cgtcatttaa tatgagctca tcagcatcga taagcacttt ggacggcaat
    22261 aagtttattg cttcagttat ggcattaaat cagaaaaaga aagcaaatgt agtttcaagg
    22321 ccggtgattt tgacacaaga gaatattccg gctatatttg ataataacag gactttttat
    22381 gtgtcattag ttggtgagcg caattcttcg ttggagcatg ttacgtatgg aacattgata
    22441 aatgttatac ctcgcttttc atctcgggga caaattgaga tgtctttaac gatagaggat
    22501 ggaacaggaa atagtcagtc aaattacaat tataataatg aaaatacatc tgtccttcca
    22561 gaagttggaa ggaccaaaat cagtacaata gcgagagttc cacaaggcaa aagtttgttg
    22621 ataggtggtt atacgcatga aacgaactct aacgaaatag tatctattcc ttttctctcc
    22681 tctattcctg taatcggtaa tgtgttcaag tataaaacaa gtaatatcag taatattgta
    22741 agggtttttc tgattcaacc ccgtgagata aaggagtcaa gttattataa tactgcagaa
    22801 tataagagcc tcatatctga aagagaaata caaaagacta cacaaataat cccatctgaa
    22861 actactttgc tggaagacga aaaatcattg gtttcatact taaattacta actataaagt
    22921 aggtgatgta tgcttgatgt taaaaataca ggagttttta gctctgcatt cattgataag
    22981 ttgaatgcaa tgacaaattc agatgatgga gatgagactg ctgatgcaga gcttgattct
    23041 ggcttggcta atagcaagta tattgactca tctgatgaga tggcttccgc tctttcgtca
    23101 tttataaaca gaagagacct tgagaaactg aaaggaacaa atagtgatag tcaggaacgt
    23161 attttagatg gggaagaaga tgaaattaat cacaagattt ttgatttaaa gagaacttta
    23221 aaagataacc ttcctctaga tcaggatttt atagacagac taaagagata ttttaaagat
    23281 ccaagtgatc aagtcttagc attaagggaa cttttgaatg aaaaagatct tactgctgaa
    23341 caagtcgaat cattaactaa aattattaat gagataatat caggtagtga aaaaagtgtt
    23401 aatgctggaa taaattcagc tatacaggct aaattatttg gcaacaaaat gaaacttgaa
    23461 ccacagcttt tgcgtgcatg ttatcgtggt tttatcatgg ggaacatatc aacaacagat
    23521 cagtatatag aatggcttgg taattttggt tttaatcaca gacatacaat tgtgaatttt
    23581 gtagagcagt cactgattgt agacatggat tctgagaaac cgagctgtaa tgcttatgag
    23641 tttggttttg tgttatctaa attaattgca attaagatga ttagaacttc agatgtaatt
    23701 tttatgaaga aactggaatc ctcaagcttg ctaaaagatg gcagtttaag tgcagagcag
    23761 ctattgctaa ctttattata tatttttcaa tatccaagtg aaagtgagca aattcttact
    23821 tctgttatag acgtatcacg agccagtcat gaggattctg tagtgtatca aacatatcta
    23881 tcttctgtta atgaaagtcc tcatgatata tttaaaagtg aaagtgaaag agaaattgcg
    23941 atcaatattc tacgagagct tgtcacaagt gcatacaaga aagagctttc tagataacag
    24001 gagatgaaag tgatccagtc ttttcttaag caagtaagta ctaagcctga attaattata
    24061 ttagtgctta tggttatgat cattgctatg ttgatcatac cattacctac ataccttgtt
    24121 gattttttaa tagggcttaa tattgtactt gctattctcg tttttatggg atcattttat
    24181 attgaaagaa tattgagttt ttctacattc ccttcggttt tgttgataac gacattattt
    24241 cgtctcgctt tatcaataag tactagtcga ctaattttag ttgatgcgga tgcgggtaaa
    24301 attattacta catttggcca gtttgtcatt ggtgatagtc tggctgtagg ttttgttatt
    24361 ttttcaatag taactgttgt gcagttcatt gttataacaa aaggatctga aagggttgcg
    24421 gaggtagctg ctcgcttttc tcttgacggt atgccgggga aacaaatgag cattgatgcg
    24481 gatttaaagg ctggaattat tgatgccgca ggagctaaag aaagacgcag tattctggaa
    24541 cgtgagagtc aattgtatgg ttcatttgat ggagcaatga agttcattaa aggtgatgca
    24601 attgctggca tcattattat ttttgtcaat ttaataggag gtatttctgt tggtatgagt
    24661 caacatggaa tgtctctatc tggtgcatta tctacttata ccatactaac tattggtgac
    24721 ggattggtct ctcagattcc tgctctgtta atttctataa gtgcaggatt tatcgttaca
    24781 agagttaatg gtgatagcga taatatggga cgtaacataa tgtcccagat atttgggaat
    24841 ccttttgttc tcattgttac atcagctctt gccttggcga tagggatgtt gccaggtttt
    24901 ccgttttttg tttttttcct gatagcagtt actttgacgg ctttattcta ttataaaaag
    24961 gtcgtagaaa aagaaaaaag tctgtctgag tctgattcta gtggctatac tggtacattt
    25021 gatattgata atagccatga ctcatctttg gcaatgatag aaaatctgga tgctattagt
    25081 tcagaaaccg ttcctttaat tttattattt gccgaaaata agataagtgc aaatgatatg
    25141 gaaggtctta ttgaaaggat aagaagtcag ttctttattg attatggtgt caggcttcca
    25201 actattttat atagaacaag caatgagctt aaggtcgatg atattgtttt gttaataaat
    25261 gaggtgcgtg ccgatagttt taatatatac tttgacaaag tgtgtattac agatgaaaat
    25321 ggagatatag atgctctagg tattcctgtt gtttcaactt catataatga acgtgttatt
    25381 tcttgggtag acgtttcata tacagaaaat ctaactaata ttgatgctaa aattaaaagt
    25441 gctcaagatg aattttatca ccagttgtca caagctttat taaacaacat aaatgagatt
    25501 tttggtatac aagaaacaaa aaatatgtta gatcagtttg aaaatcggta tcctgatcta
    25561 ttaaaggaag tcttccgaca tgtgactata cagagaattt ctgaggtatt acaaagattg
    25621 cttggagaaa atatttctgt tcgcaattta aaacttatta tggagtcttt ggcgctttgg
    25681 gctcccagag aaaaagatgt tataacatta gttgaacatg tccgtgcatc actttctagg
    25741 tatatttgta gtaaaatagc tgtttctggt gagattaaag ttgtgatgct ttccggatat
    25801 attgaggatg caataagaaa agggataagg caaacctctg gtggctcttt cttgaatatg
    25861 gatatagagg tttcggatga ggtaatggaa actttagcac atgctttgag agaattgaga
    25921 aatgcaaaaa aaaatttcgt tcttttggta tcagtagata tacgtaggtt tgttaaaaga
    25981 cttatagata acagatttaa gagtatactc gttatatcgt atgctgagat tgatgaagca
    26041 tataccatta atgtattaaa gactatttag tgaggtttaa atatgagtaa cattaattta
    26101 gttcaattag ttagagatag tcttttcacg attggttgtc ctccatcaat tattacagat
    26161 ttagacagcc attcagcgat taccatttct ttggatagta tgcctgctat taatatagct
    26221 cttgtcaatg agcaggttat gttatgggct aattttgatg caccaagtga tgtaaaactt
    26281 cagtcatctg cttataacat attaaattta atgttaatga actttagtta tagcattaat
    26341 gagctggtag agcttcatcg ttctgatgag tacttacagt taagagttgt tattaaagat
    26401 gactacgttc acgatgggat tgtttttgca gagattctgc atgagtttta tcagaggatg
    26461 gaaatcttaa atgaggtctt ataatcaatg agctatataa aattgctcac tcaattatct
    26521 tttcctaata gaatctcggg gccaatcttg gaaacaagtc ttagcgatgt ttcgattggt
    26581 gagatttgta acattcaggc tggaattgaa agtaatgaaa ttattgcaag agctcaggtt
    26641 gtaggatttc atgatgaaaa aacaatatta agcttgattg gaaattctcg tggactttca
    26701 cggcaaacgt tgattaagcc cactgcccag tttcttcata cgcaagtcgg ccgtggatta
    26761 ttgggagcag tagtcaatcc tttaggggag gttactgata agtttgctgt tacggataac
    26821 agtgaaattc tttatcgacc tgtagataat gctcctccgc tatatagtga aagggctgca
    26881 attgagaagc cttttttaac aggtgttaag gttattgatt ctttactcac gtgtggtgaa
    26941 ggacagcgaa tggggatttt tgcgtcagct ggttgtggca aaacttttct catgaatatg
    27001 ctcattgaac atagtggtgc tgatatatat gttattgggt taattggtga gcgaggtcga
    27061 gaggttacag aaacggttga ttatttgaaa aactctgaga aaaaaagcag gtgtgtttta
    27121 gtatatgcaa cttcggatta ctcttcggtt gatcgttgta atgctgcata tatagccact
    27181 gctatagccg aattttttag gactgaagga cataaagtag cgctttttat tgattcatta
    27241 acaaggtatg ccagagcatt acgtgatgtg gccttagcag ctggagaatc acctgccaga
    27301 agaggctatc cggtttcggt ttttgatagc ttacccagac ttcttgaaag gccaggaaag
    27361 ttaaaggcag gtggctctat tactgcattt tacactgttc ttttggagga tgatgatttt
    27421 gctgatccat tagctgaaga ggtaagatcc attttagatg gacatatata tttgagcaga
    27481 aatctagccc aaaaaggaca atttcctgca attgattcct taaaaagtat aagcagggta
    27541 tttacacagg ttgttgatga aaaacatcgt attatggccg ctgcatttcg ggagctactt
    27601 tctgaaatag aagagctaag gacaattatt gactttggtg aatacaaacc gggggagaat
    27661 gcctctcagg ataaaatcta caacaaaata tctgttgttg agagttttct gaagcaagat
    27721 tatcgactcg gttttactta tgagcagaca atggagctta ttggtgaaac aattagataa
    27781 ggttttaaaa atcaaagata aatatcaacg gtctgttaaa ttaattgaag cacacatatt
    27841 aacactaaaa aaaaaactct ttgtatcgag atgtggaggc attagataaa aggattatat
    27901 acttccagca actggagaat gacttggaac ctgtaggcgc tcagagtgtt agtcagcttt
    27961 tcaatacaag acgtaagatc gctatagtaa aaaagcatat tatccaatat cagtctgaac
    28021 gaattcttct aaagggaaga attgaagaaa ttcaaaaaga tattgatgag gcaaatgctt
    28081 ctaaaagaaa actgcttcat aaagagagta aaatatgtaa acggattggc ttgattaaaa
    28141 gaaataactt tgccaaacag ttgatacttg atgagttatc acaagaggat atgaagtatg
    28201 gcattagata atataaacct aaattttagt agtgacaaac agatagaaaa atgtgagaaa
    28261 ctatcttcaa tagataatat tgatagtctc gttttgaaga agaagaggaa ggttgaaatt
    28321 cctgagtact ctttaattgc atctaactat tttactattg ataagcactt tgaacataag
    28381 catgataaag gagaaattta tagtggcatt aaaaatgcgt tcgaacttag aaacgaacga
    28441 gcgacatatt ctgatattcc agaatcaatg gccattaaag aaaatatttt gataccagat
    28501 caagatatca aagcaaggga aaaaataaat atcggcgata tgagggggat cttttcatat
    28561 aataagagtg gaaatgcaga caagaacttc gaaagaagtc atacttcttc tgtaaaccct
    28621 gataatctgc tagaatctga taatagaaat ggtcaaattg gtttaaaaaa tcatagcttg
    28681 tctattgata agaatattgc tgacatcatt tctttactaa atggaagtgt tgctaaatca
    28741 tttgagctgc ctgtaatgaa taaaaatact gcagacataa ccccatccat gtcattgcaa
    28801 gaaaaatcaa tagttgaaaa tgataaaaat gtttttcaaa aaaatagtga aatgacttac
    28861 cactttaaac agtggggggc tggacattct gttagtattt cagtggagtc tggttctttt
    28921 gttctaaaac cgtcagatca atttgtagga aataaacttg acttaatttt gaaacaagat
    28981 gctgagggta attacagatt tgatagcagt caacataata aggggaataa aaataatagt
    29041 acaggatata atgaacagag tgaagaagaa tgctaagaat taaacatttt gacgctaacg
    29101 aaaaactaca gattttatat gcaaagcaac tctgcgagcg tttttcgatt cagacattca
    29161 aaaataaatt tacaggcagt gaaagtttag tcactcttac ttccgtgtgt ggggattggg
    29221 taattcgtat tgatacatta tcttttttga aaaaaaaata cgaggtattt tcaggatttt
    29281 ctacacaaga atctttactg catttatcaa aatgtgtctt tatagagtcg tcatctgtat
    29341 tttcgattcc agaactgtct gataagatta ctttccggat cacgaatgaa atccagtatg
    29401 caactactgg gagtcattta tgctgttttt cctcttcttt aggtattatt tattttgaca
    29461 agatgccggt attacgtaat caagtttctc ttgacttatt gcatcatctt ttagagtttt
    29521 gcttaggttc atctaatgta aggctggcta ctttaaaaag aattcgtact ggtgatataa
    29581 tcatagttca gaaactttat aatttattat tgtgtaatca agttattatt ggggattata
    29641 ttgtgaatga taataatgag gcaaaaatta atctgtcaga aagtaatggt gagtcagatc
    29701 acacagaagt ttctttggca ttattcaatt atgatgatat caatgtaaaa gtggacttta
    29761 tccttttaga aaaaaagatg acaatcaatg aactaaaaat gtatgtagaa aacgaattat
    29821 tcaagtttcc cgatgacata gttaaacatg taaatattaa agtaaatggt tctttggttg
    29881 ggcatgggga acttgtttct attgaggatg gttatggtat cgagattagt tcttggatgg
    29941 taaaggagta atctcatgct gagtgatatg tccctcatcg ctacactttc tttttttacg
    30001 ctattaccat ttttagttgc tgctggtact tgttatataa agttttctat tgtttttgta
    30061 atggtacgaa atgcacttgg tttgcagcag gttccatcaa atatgactct caatggtatt
    30121 gcattgatta tggctttgtt cgtgatgaaa cccattattg aagctggtta tgaaaattat
    30181 ctaaatggcc cgcaaaaatt tgatacaata tcagatattg tccgtttttc tgattctggc
    30241 ttgattgaat acaagcaata cttgaaaaag catacagatc ttgagttggc acgttttttc
    30301 caaaggtctg aagaagaaaa tgctgattta aagagtgcag agaataatga ttattcattg
    30361 ttttccctct tgcctgcata tgcgctaagt gaaattaaag atgcgttcaa aataggtttt
    30421 tatttatatt tgccatttat tgttgttgat cttgttattt caagcatttt acttgcactt
    30481 ggtatgatga tgatgagtcc aatcacaata tcagtaccaa taaaacttgt attatttgtt
    30541 gcgcttgatg ggtgggggat cctgtctaag gccttgattg aacaatatat caatgttcct
    30601 gcttagatga tggaggtaat gtgtctgata tagtttatat gggtaataag gctctttatt
    30661 taatccttat cttttcctta tggccagtag gtatagctac ggttattgga ttaactattg
    30721 gtttattaca gacagtgact caacttcaag agcagacact tccttttggt ataaagctta
    30781 taggtgtctc aatatctttg ctacttcttt ctggatggta tggtgaggtt ttattgtctt
    30841 tttgtcatga aataatgttt ttaattaaga gtggggtttg atggacattt caagctggtt
    30901 cgaaagtatt catgtgtttt taatactcct gaacggcgtt ttttttagat tggctccatt
    30961 atttttcttt cttccatttt taaataacgg tataatttct ccatctatta gaatacctgt
    31021 gatttttctt gttgcatcag gattaattac ttctggtaag gtagacatag gttcttctgt
    31081 ttttgaacat gtttatttcc ttatgttcaa ggaaataatt gttggcctcc ttctctcttt
    31141 ttgcttgtct cttccctttt ggatatttca tgctgttggt agcattattg acaaccagcg
    31201 tggggcaacg cttagtagtt caattgatcc tgccaatggt gttgatacgt ctgagttggc
    31261 aaaatttttc aatctttttt ctgcagttgt atttctatac agtggtggaa tggtctttat
    31321 tttagaatcc atacaattgt cttataatat atgcccgtta ttttctcaat gttctttccg
    31381 tgtctcaaat atcttaacat ttctgacttt attggcaagt caggctgtta ttttagccag
    31441 tcctgttatg atagtattgt tactatcaga agtattactt ggtgtattat cgagatttgc
    31501 tccgcagatg aatgcttttt ccgtatcatt aactattaaa agtttacttg caatatttat
    31561 tattttcatc tgttcttcta ctatttactt ttctaaagtt caatttttcc tcggtgaaca
    31621 taaatttttc acaaatctat ttgttagata aaatattatg gcaaataaaa cagaaaagcc
    31681 gacacctaaa aaactaaagg atgccgcaaa aaaaggacag tcatttaaat ttaaggattt
    31741 aacgactgtt gttattattc tggtagggac atttactata atatcattct tttccttaag
    31801 tgatgtaatg ctcttataca gatatgtaat aattaatgac ttcgaaatta atgagggtaa
    31861 atactttttt gcagtggtta ttgtcttttt taagataatt ggcttcccac tttttttctg
    31921 tgttctttcg gctgtgttgc caacattggt tcaaacaaag tttgttcttg cgactaaagc
    31981 tatcaagatt gatttttcag tattaaaccc tgttaaaggg ttaaaaaaaa tatttagtat
    32041 aaagacaata aaagaatttt tcaaaagcat tctgcttctt attattctag cattaacaac
    32101 ctatttcttt tggattaatg accgaaaaat aattttttct caggtgtttt ctagtgttga
    32161 tggcttatat cttatttggg gggggctgtt taaggatata atattatttt tcttggcatt
    32221 ttctattttt gttattattc ttgactttgt gattgagttc attttataca tgaaagatat
    32281 gatgatggat aaacaggaga taaaaagaga atatatagag caagagggac actttgagac
    32341 aaagtcgaga aggcgtgagt tgcatatcga gattctttca gagcagacta aatctgatat
    32401 acgtaattca aaattagtgg taatgaaccc gactcatatt gcaattggta tttattttaa
    32461 tccagaaata gcgcctgcac cttttatttc tctcattgaa actaaccagt gtgccttggc
    32521 tgtcagaaaa tatgcaaatg aagttggtat accaactgtg cgtgatgtga aattagctag
    32581 aaaactatat aaaacacata caaaatatag ttttgttgat tttgaacact tggatgaagt
    32641 cctacgtctt attgtttggc ttgagcaggt tgaaaacact cattaataag taaaggagat
    32701 gtgttatatg ggagttaatt tctgtaataa aataggtatt gatcagtctg aatttgaaat
    32761 agaatcttcc atcattaact ccattgctaa tgaggtattg aacccaatat ctttccttag
    32821 caacaaggat ataataaatg ttttactcag aaagatttca tctgaatgtg acctagttag
    32881 aaaagacatt tatcgttgtg ctctggagtt ggtcgttgaa aaaacacctg atgatttata
    32941 atcattcata ataactcttt tgctgatata aatatttttg ttgaagatat ttatgtagtc
    33001 acaggatcaa ttgtagaaaa ttgttatgtt tttgttagga actgcactaa ataatatatg
    33061 ataagacagc aaaaacgact gaccataatc cttcttttat taggagttga taagagagac
    33121 tattcatcct gtaatgttaa aactctgtta tatagtattc gcgattatgc aaagtctgta
    33181 aatgatcatg agatactaac cgagagtaat cgtttattat ctcattgcat ttcagatagt
    33241 aacggagctt tctttaaaag tagtaaatac gttcccctta agtacttacg taaaaggaga
    33301 atagcacgaa agataccaaa tgattagcac tttgctagta agcttgctgg taaactatat
    33361 tgaccgttgg gtttgttttg agactgtaat ttaaattgtg taattgcctg tttttgatat
    33421 gtttactcca acaacggaga caggtaaatt atggacgaaa agaagcttaa agcccttgcg
    33481 gctgaactgg ctaaaggcct caaaaccgag gccgacctca atcagttttc ccgtatgcag
    33541 acgaaattaa ctgtcgaaac ggtgcttaat gcggaactaa ctgaccatct cgggcatgaa
    33601 aaaagctaca tccggtagta aacattatca gtgggttatg cgatatgat
//
LOCUS       DADLSA010000032        32297 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.032, whole genome shotgun sequence.
ACCESSION   DADLSA010000032 DADLSA010000000
VERSION     DADLSA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.032"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..848
                     /locus_tag="JN691_001507"
     CDS             <1..848
                     /locus_tag="JN691_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7242983.1"
                     /translation="KEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGA
                     QLTFIARRDVTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLAPDPA
                     ALKEAPDDATFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTF
                     NPAHAQQKIASADLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDA
                     QLRKADPVAWGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLR
                     RYGTH"
     gene            821..2357
                     /locus_tag="JN691_001508"
                     /pseudo
     CDS             821..2357
                     /locus_tag="JN691_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2357..3010
                     /locus_tag="JN691_001509"
     CDS             2357..3010
                     /locus_tag="JN691_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY7242984.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            3077..4384
                     /locus_tag="JN691_001510"
     CDS             3077..4384
                     /locus_tag="JN691_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HAY7242985.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPVGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVENEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPGSSK"
     gene            complement(4393..5013)
                     /locus_tag="JN691_001511"
                     /pseudo
     CDS             complement(4393..5013)
                     /locus_tag="JN691_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
     gene            5100..5507
                     /gene="nudG"
                     /locus_tag="JN691_001512"
     CDS             5100..5507
                     /gene="nudG"
                     /locus_tag="JN691_001512"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HAY7242986.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(5473..5745)
                     /locus_tag="JN691_001513"
     CDS             complement(5473..5745)
                     /locus_tag="JN691_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HAY7242987.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            5981..7324
                     /gene="gdhA"
                     /locus_tag="JN691_001514"
     CDS             5981..7324
                     /gene="gdhA"
                     /locus_tag="JN691_001514"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAY7242988.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(7441..8481)
                     /locus_tag="JN691_001515"
     CDS             complement(7441..8481)
                     /locus_tag="JN691_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7242989.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSATLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(8609..10570)
                     /locus_tag="JN691_001516"
     CDS             complement(8609..10570)
                     /locus_tag="JN691_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY7242990.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(10575..11618)
                     /gene="selD"
                     /locus_tag="JN691_001517"
     CDS             complement(10575..11618)
                     /gene="selD"
                     /locus_tag="JN691_001517"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAY7242991.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(11735..12286)
                     /locus_tag="JN691_001518"
     CDS             complement(11735..12286)
                     /locus_tag="JN691_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAY7242992.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGGERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            12447..14303
                     /gene="sppA"
                     /locus_tag="JN691_001519"
     CDS             12447..14303
                     /gene="sppA"
                     /locus_tag="JN691_001519"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAY7242993.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSPHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            14470..15486
                     /gene="ansA"
                     /locus_tag="JN691_001520"
     CDS             14470..15486
                     /gene="ansA"
                     /locus_tag="JN691_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAY7242994.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNASQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            15497..16138
                     /gene="pncA"
                     /locus_tag="JN691_001521"
     CDS             15497..16138
                     /gene="pncA"
                     /locus_tag="JN691_001521"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAY7242995.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16231..17589)
                     /locus_tag="JN691_001522"
     CDS             complement(16231..17589)
                     /locus_tag="JN691_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7242996.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(17707..18465)
                     /locus_tag="JN691_001523"
     CDS             complement(17707..18465)
                     /locus_tag="JN691_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7242997.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(18602..19582)
                     /gene="ydjG"
                     /locus_tag="JN691_001524"
     CDS             complement(18602..19582)
                     /gene="ydjG"
                     /locus_tag="JN691_001524"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HAY7242998.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKLIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(19592..20539)
                     /locus_tag="JN691_001525"
     CDS             complement(19592..20539)
                     /locus_tag="JN691_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HAY7242999.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(20544..21380)
                     /locus_tag="JN691_001526"
     CDS             complement(20544..21380)
                     /locus_tag="JN691_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAY7243000.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            complement(21401..22444)
                     /locus_tag="JN691_001527"
     CDS             complement(21401..22444)
                     /locus_tag="JN691_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HAY7243001.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(22461..23840)
                     /locus_tag="JN691_001528"
     CDS             complement(22461..23840)
                     /locus_tag="JN691_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7243002.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLLAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(23867..24943)
                     /locus_tag="JN691_001529"
     CDS             complement(23867..24943)
                     /locus_tag="JN691_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7243003.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSSANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(25313..25585)
                     /locus_tag="JN691_001530"
     CDS             complement(25313..25585)
                     /locus_tag="JN691_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HAY7243004.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(25627..26040)
                     /gene="msrB"
                     /locus_tag="JN691_001531"
     CDS             complement(25627..26040)
                     /gene="msrB"
                     /locus_tag="JN691_001531"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAY7243005.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            26382..27377
                     /gene="gapA"
                     /locus_tag="JN691_001532"
     CDS             26382..27377
                     /gene="gapA"
                     /locus_tag="JN691_001532"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY7243006.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            27461..28345
                     /locus_tag="JN691_001533"
     CDS             27461..28345
                     /locus_tag="JN691_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAY7243007.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(28396..29157)
                     /locus_tag="JN691_001534"
     CDS             complement(28396..29157)
                     /locus_tag="JN691_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAY7243008.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(29247..29993)
                     /locus_tag="JN691_001535"
     CDS             complement(29247..29993)
                     /locus_tag="JN691_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAY7243009.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            30429..>32297
                     /gene="yeaG"
                     /locus_tag="JN691_001536"
     CDS             30429..>32297
                     /gene="yeaG"
                     /locus_tag="JN691_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAY7243010.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEK"
BASE COUNT         8107 a         8368 c         7753 g         8069 t
ORIGIN      
        1 taaaagaggc caatttatta caaacgggct gggcggagac tctgcccaac tggcgctatg
       61 tcgacacaca gctgccggtg cgggaagatt tttcagtgcc gacacaaggt gcggaatcgc
      121 cctggggcgg cgcacaactg acgtttatcg cccgccgcga tgttacgcca cagccaccac
      181 aaacgccgca agccttactg gagtttgcta aagccaatcc cggcacggtt acttatccgc
      241 gcccaccgga ctttaccggc acggcgtttc ttgaacagtt gctgattatg ctggcgccag
      301 atcccgccgc attaaaagaa gcgccggacg atgcgacttt cgcccgtgtc actgcgccct
      361 tgtggcaata tcttgatgca ctgcatccgt atttgtggcg cgaaggaaag gatttcccgc
      421 cttcgcccgc gcggatggat gctctgctga aagccggcac attgcgcctg tcgctgacct
      481 ttaaccccgc gcatgcgcag caaaaaatcg ccagcgccga tttgccggca agcagttaca
      541 gttttggctt tcgcgagggg atgattggca acgtgcattt cgtcaccatt ccagccaacg
      601 cgaatgccag tgctgcggcg aaggtagttg ccaatttcct gctctcaccc gatgcgcaac
      661 tgcgtaaagc agatcccgtt gcctggggcg atccttctgt tctcgatccg caaaaactgc
      721 ctgacgggca gcgcgaaata ttgcaatcaa gaatgccgca ggatctgccg ccggtactgg
      781 ctgaaccgca cgcaggatgg gtgaatgcac tggaacaaga atggttacgc cgttacggta
      841 cgcattaatc tttttgctgt gggcgatggt ggcggtgatt tatgcaccgc tgatcccggc
      901 agctctcacg ctgatatcgc ctgcgttgtc gttgacacgc tggcaggcgt tatttgccga
      961 tccacagtta ccgcaggcat tactggcaac gctggtgtcg acaaccatcg cggcggtcgg
     1021 ggcattgttg attgccctgc tggtgattgt ggcgctatgg cctgggccga aatggcagcg
     1081 tatgtgcgcc cgtctgccgt ggctgctcgc cattccccat gtggcttttg ccaccagcgc
     1141 cctgctgctc tttgctgacg gagggctgct atatgactat ttcccgtatt tcactccgcc
     1201 aatggaccga tttggcatcg gtctgggcct cacccttgca gtgaaagaaa gcgcatttct
     1261 gctgtggatc ttagcggcag tattgagcga aaaacggctg ttgcagcagg tcattgtgct
     1321 ggattcgctg ggctacagcc gctggcaatg cctgaactgg ctgctgttgc cctccgtcgc
     1381 gccttcgctg gcaatggcga tgctggcgat tgttgcctgg tcgctttcgg tcgtggatgt
     1441 ggcgattatt cttgggccag gtaatccacc gacgctggca gtaattagct ggcagtggtt
     1501 aactcagggc gacgcggatc aacaaacgaa aggcgcactc gccagtctgc tgctgatgct
     1561 gttactcgcc gcctacgttt tgctgagcta tctgctatgg cgcagctggc ggcgcactat
     1621 tccccgcgta gatggcattc gcaatgcctg ccacaccttt attaccaggc actacgctgg
     1681 cgagtttttt acccttaacc ggtgtgctgt gtgtggttct gctggcgatc ctcgcggatc
     1741 agtcgacgat caatagtgaa gcactcatca acagtctgac aatggggctg gtagcaacat
     1801 tcatcgcttt gctcctgtta ctgctgtggc tggaatgggg ggcacagcgt cgccagttgt
     1861 ggctatggtt gcccattcta ttgcccgctc tgccactggt ggcgggccag tacacgctgg
     1921 cgctatggct gaacctggat ggaagctgga cggcggtggt ctgggggcat ctgctgtggg
     1981 taatgccgtg gatgctgttt atcctgcaac ccgcctggca gcgcattgat tcacggttaa
     2041 ttttgattgc gcaaacactg ggctggtcgc gggccaaaat cttcttttac gtgaaatgcc
     2101 cactcatgtt gcgccctgtg ctgattgcct tcgcggtggg attttcagtc agtattgcgc
     2161 agtatatgcc gacgctgtgg ctgggcgcgg ggcgttttcc gacgctcacc actgaggcgg
     2221 tggcattaag cagcggcggc agcaacggta ttctcgccgc ccaggcttta tggcaactgt
     2281 tgttaccgct tattattttt gccctgaccg cgttagtcgc aaaatgggta ggttatgtca
     2341 gacaaggaat ccgctaatgc tctgcgtgaa aaatgtttcg ctacgtttac cagaaagccg
     2401 cttgctgaca aacgttaact ttacggttga taaaggtgac atcgtcacgt taatggggcc
     2461 gtctggctgt ggaaaatcca ctctgttttc atggatgatt ggtgcactgg ccggacagtt
     2521 ttcttgtaca ggtgagctat ggctcaatga gcaacggatt gacatgctac ccaccgcaca
     2581 gcgtcagatt ggcattcttt ttcaggatgc actgttattt gaccagttca gcgtcgggca
     2641 aaatttactg ctggcgctac cggcgacact taaggggaat gcccgacgta atgccgtgaa
     2701 tgatgcactt gagcgttcag gccttgacgg agctttccat caggatcctg ccactttgtc
     2761 tggcggtcag cgagcgcgcg ttgctctact acgcgccctt ctcgcccagc caaaagcgtt
     2821 actcctggat gagccattca gccgtcttga tgtggctctg cgcgataatt ttcgccagtg
     2881 ggtgttcagc gaagttcgcg ccctggcgat ccccgtcgtt caggtaacgc acgatctcca
     2941 ggatgttcct gctgatagtt ctgttctgga tatggcgcag tggtcagaaa attacaacaa
     3001 actgcgataa cgcaaagttt ttctcaatgc gtcagttcag aatggcgcac tcaaaactac
     3061 aatgtcggga ttttcgatga aacgtgtttc tcaaatgacc gcgctggcaa tggctttagg
     3121 gctggcttgc gcttcttcgt gggccgctga actggcgaag cctcttacac ttgaccaact
     3181 tcagcaacaa aatggcaaag cgatagatac tcgccccagc gcgttttata acggctggcc
     3241 acaaacctta aatggccctt ctggtcatga acctgccgcc ttaaacctct ctgctagctg
     3301 gctcgacaaa atgagcaccg aacagctcaa cgcgtggatc aagcaacata acctgaaagc
     3361 cgatgccccg gtggcgctgt acggtaatgg caaagatgtc gacgccgtca aaacgcgact
     3421 gcaaaaagca ggttttacgc atatctccat tctgagtgac gcgctaagcg aaccttcccg
     3481 tctacaaaaa ctgccgcact ttgagcagct ggtttatccg caatggctgc atgacctgca
     3541 acaaggtaaa gaggttacgg cgaaacctgt cggtgactgg aaagtcattg aagcggcctg
     3601 gggcgctcct aagctttacc ttatcagcca tattcctggc gctgactaca tcgataccaa
     3661 cgaagtggaa aacgaaccgc tgtggaacaa agtttctgat gaacaactaa aagcgatgct
     3721 ggcaaaacac ggcattcgcc atgacaccac ggtcattctg tacgggcgtg acgtatacgc
     3781 tggagcgcgt gtggcgcaaa ttatgctcta cgcaggcgtg aaagatgtgc gtctgctgga
     3841 tggcggctgg caaacctggt ccgacgcggg actgcctgtt gagcgaggaa cgccaccgaa
     3901 agtgaaagcg gaaccggatt tcggcgtgaa gatcccggca caaccgcagt tgatgctcga
     3961 tatggaacag gcccgtggac tgctgcatcg ccaggatgca tcgctggtga gcattcgttc
     4021 gtggccagaa tttatcggta cgaccagcgg ttacagctat attaaaccaa aaggtgaaat
     4081 agccggagca cgttggggac acgctggtag cgactcgacg catatggaag atttccataa
     4141 cccggatggc accatgcgta gcgccgatga tattaccgct atgtggaaag catggaatat
     4201 caaaccagat cagcaagttt cattctactg cggcaccggc tggcgcgcgt ccgaaacctt
     4261 tatgtacgca cgcgccatgg gttggaagaa tgtttccgtg tatgacggcg gctggtacga
     4321 atggagcagc gatccaaaaa atccggttgc aaccggtgaa cgcggcccgg gcagtagcaa
     4381 ataacattgc aattactgac gctggagcga cttcagcgtc agatacccac tccacacccg
     4441 cgtaaaggtc gtcatccagc acaacgcacc gaagattcac gcaaaccacg caaaccatgc
     4501 agggaataaa catcccagca caaacagtaa gatcgtttct gtgccttcgg ttaatccccc
     4561 cagatagtaa aacgacttat gtgcataacc ggggttatca atctgatgtt ttgctgccag
     4621 tgcagcaaag gcgagaaaac tgctgcccgt gccgataaac gcaaacaaca accagccgcc
     4681 tgccagcgca ttttgctccg gtgcagcaag aataaagcca aacggcacca gcgcgtaaaa
     4741 gagaaaatcg agagaaatat caagaaagcc gcccgcgtcg gtaagccctc tgcgccgcgc
     4801 cagcgcgccg tccagaccat caagcagcct gttcaacaaa atgacgacta gcgccgccag
     4861 ataccagccc agcgccagaa acggcagagc cagcacgccg atggcaaatc ccactaatgt
     4921 taaaccatct ggcgtaatgc ccggtttatc aagaacccgc acgcactgat gcaacaacgg
     4981 tttaatccgg ggatgaagat ggcggtctag cacaggcact ccttaaatat aaagcctttc
     5041 tgattgagca acagtgcgga tattatggca tttttcgctt atctgcccgt gtgtaattta
     5101 tgaaaatgat tgaagttgtt gccgccatca ttgaacgtga tggcaaaatt ttactcgcgc
     5161 aacgccccgc ccagagcgat caggcgggat tatgggagtt tgccggtggt aaagtcgagc
     5221 tggatgaaag ccagcagcag gcgctggtgc gtgagttaaa cgaagaattg ggcatcgaag
     5281 caactgtggg tgagtatgtt gccagccatc agcgagaagt ttcgggccgg attatccatc
     5341 ttcatgcctg gcacgtaccc gacttccacg ggacgttaca ggcacatgaa catcaggcgc
     5401 tggtctggtg ctcacctgaa gaggcgctgc aatatccgct ggcccctgct gacattccat
     5461 tattagaggc gtttatggct ttacgcgccg ccagagcagc ggattagtgc taagggtttt
     5521 gtcatcacgc tggcattgca gcagtattcc ttcggcttta attaccgccc cttcagaata
     5581 attttgatcc tgataaacgc agcactgagt acagggctgc gctgactgcc cgcctgaact
     5641 gaacacttct ggcggtacgt ttacctccac gtccggacga taatgcgggt tagccagtgc
     5701 aattaatgga aatgctaata ctacggcgaa caatgctcga ctcacaggga actccttaac
     5761 gttattgtct ctgctactga taacggtagc cgggtggcaa aactttagcg tctgagttat
     5821 cgcatttggt tatgagatta ctctcgttat taatttgctt tcctgggtca tttttttctt
     5881 gcttaccgtc acattcttga tggtatagtc gaaaactgca aaagcacatg acataaacaa
     5941 cataagcaca atcgtattaa tatataaggt ttttatatct atggatcaga catattctct
     6001 ggagtcattc ctcaaccatg tccaaaagcg cgacccgaat caaaccgagt tcgcgcaagc
     6061 cgttcgtgaa gtaatgacca cactctggcc ttttcttgaa caaaatccaa aatatcgcca
     6121 gatgtcatta ttggagcgtc tggttgaacc ggagcgcgtg atccagtttc gcgtggtatg
     6181 ggttgatgat cgcaaccaga tacaggtcaa ccgtgcatgg cgtgtgcagt tcagctctgc
     6241 catcggcccg tacaaaggcg gtatgcgctt ccatccgtca gttaaccttt ccattctcaa
     6301 attcctcggc tttgaacaaa ccttcaaaaa tgccctgact accctgccga tgggcggtgg
     6361 taaaggcggc agcgatttcg atccgaaagg aaaaagcgaa ggtaaagtga tgcgtttttg
     6421 ccaggcgctg atgactgaac tgtatcgcca cctgggcgcg gataccgacg ttccggcagg
     6481 tgatatcggc gttggtggtc gtgaagtcgg ctttatggcg gggatgatga aaaagctctc
     6541 caacaatacc gcctgcgtct tcaccggtaa gggcctttca tttggcggta gtcttattcg
     6601 cccggaagct accggctacg gtctggttta tttcacagaa gcaatgttaa aacgccacgg
     6661 tatgggtttt gaagggatgc gcgtttccgt ttctggctcc ggcaacgtcg cccagtacgc
     6721 tatcgaaaaa gcgatggaat ttggtgctcg tgtgatcact gcgtcagact ccagcggcac
     6781 tgtagttgat gaaagcggat tcacgaaaga gaaactggca cgtcttatcg aaatcaaagc
     6841 cagccgcgat ggtcgagtgg cagattacgc caaagaattt ggtctggtct atctcgaagg
     6901 ccaacagccg tggtctgtac cggttgatat cgccctgcct tgcgccaccc agaacgaact
     6961 ggatgttgac gccgcgcatc agcttatcgc caatggcgtt aaagccgtcg ctgaaggggc
     7021 aaatatgccg accaccatcg aagcgactga actgttccag caggcaggcg tactgtttgc
     7081 accgggtaaa gcggctaatg ctggcggtgt agcaacgtcg ggcctggaaa tggcacaaaa
     7141 cgctgcgcgc ctgggctgga aagccgagaa agttgacgca cgtttgcatc acatcatgct
     7201 ggatatccac catgcctgtg ttgagcatgg tggtgaaggt gagcaaacca actacgtgca
     7261 gggcgcgaac attgccggtt ttgtgaaggt tgccgatgcg atgctggcgc agggtgtgat
     7321 ttaagttgta aatgcctgat ggcgctacgc ttatcaggcc tacagatggg cacaattcat
     7381 tgcagttacg ctctaatgta ggccgggcaa gcgcagcgcc cccggcaaaa tttcaggcgt
     7441 ttatgagtat ttaacggatg atgctcccca gggaacattt cttatgggcc aacggcattt
     7501 cttactgtag tgctcccaaa actgcttgtc gtaacgataa cacgcttcaa gttcagcatc
     7561 cgttaacttt ctgcgatagc agcagatatg ccagtaaaga aatcccatta gactattttt
     7621 ttgataatct tcttcgcttt cgaacaactc gtgcgccttt cgagaagcaa gcattatata
     7681 atgccaggcc agttcttctt caattgtccc gttttgaaaa gctgtgcttg atatcgagat
     7741 catccatgat aattccgccg cccatattag cttcgccgag gatttaccgg agcgattatt
     7801 agcgcaatca gagatatagt ctgagggaaa aacagcaaat ttattcaaca aggcgataac
     7861 ctgctctggg gcttcctcca tgtttgcttt aaaggtattg gctccatggt cgccagaaag
     7921 aaaatgctcc attaaggcac aataactttc gctatcttcg ataccccatt gattctctaa
     7981 agactcgcgt cttttactga tgatatcgat cgagtcaaaa ggaagcacat gatattggaa
     8041 ggtatctttg ccaggttcag gctttcgcgg ccagaactct agcgttgcag accattgctt
     8101 atgatagaat cgataaggtg cgatcaattg tagcgcctgt aacttctcga tactgagcgg
     8161 ctcaatacct ttagcctgat aataatgcag ttgttctttt tttgctttaa aaccggcccg
     8221 aacaataagc cccatcataa ttaatagata aagaaaagag catcccgcgg taatcaggcc
     8281 tctttcattc aaaccgttgg atgttatcgc tgcgaataca aacattacag cgacaacaca
     8341 tgttaaataa aacccccact tacaaagcag catggcctta ttttctttaa tcacccgttc
     8401 aaaattacta ttaaatattt cccagccatt aaaagaatac ttctcgctcc caggatggtt
     8461 ttgtaataaa acttttttca tcacattccc tgttattaca tactttagag gcgataattt
     8521 tatccatgca aaaaaaatat ccaacgaatt ttcttgatcc gctggatatt tcagaatatg
     8581 actcgaatag cacgaaagat tcgctcgctt acgctatcgc cccgcttccg gcttcatctg
     8641 ctggcggact ttttttcgca ctacgtttac gcggtgcagc ctttttctta tcagcactgc
     8701 caccactgcc cggagccaca atgccgcgaa actgccgcac cggcgtacgt ttggcttgat
     8761 caataagctg atatagcgtc cccaccagcg gctgcataaa gtcctgatag cgacactgct
     8821 tttcgctgat ttgcgtcagc accgattccc agtgcgcggt catgtccggt cgcgtcgcca
     8881 tttctggcag cgaatggaat aacgcttttc cggcgtcggt ggagtggata tagcgccctt
     8941 ttttggtcag aaaaccacgc ttgaacagca gttcaataat cccggcacgg gttgcttctg
     9001 tccccagacc atcggtcgca cgaaggacct ttttcagatc tttgtcctgc acaaagcgcg
     9061 cgatcccggt catcgccgaa agcagtgttg catcggtaaa atggcgcggc ggctgggttt
     9121 gccgctctac cacttcacct ttttcacaca gcaactcatc gcctttcgcc accacaggca
     9181 atggcgtgcc gtcgttttct tcatcgcgct ctttgctgcc taacagcgtg cgccagcctg
     9241 cttcagcaag aaaacgggct ttagcgacaa atttgccttt ggcaatgtcc agttcgataa
     9301 cacacttgcg gaacaccgca tccgggcaga actgcatcag gtattgacgg gcaatcaggt
     9361 tatagacctt cgcttcgttc tccgtcaggt tgatcgcaga actccgtgcg gtcgggatga
     9421 tggcgtggtg cgcatcgacc tttttgtcat cccaacagcg gttgcgtata tctggatcta
     9481 ccactggctg cggcaacaga tccggtgcat gaacgctgat ggcattcatc accgcgtggc
     9541 gtccggcaaa atgttcttct ggcaaatagc gacaatcgga acgcggataa gtgattagct
     9601 tgtgcgtttc gtacagcttc tggcagatat caagcacgtt ctgcgcgctc agaccgaagc
     9661 gttttgccgc ttcaatctgc aacgccgaaa gcgaaaacgg cagcggcgcg gattctgatt
     9721 cccgtttatc gttatagctg gtgacaatag ccggttgacc actaatgcgg ttaaccacat
     9781 gctccgccag tggacgatgt aacaagcgcc cttcttcatc ctggtacggt tcacacgctt
     9841 cgctcggttg ccagatagcg gtaaaccgct catcggcagg tgtcacgata tgtgctttga
     9901 cttcaaagaa gtctttcgcc acgaagtttt caatctcttc atcgcggcgc accaccagcc
     9961 cgagcacggg tgtctgcacg cgtcccacgg aaagtacgcc ctgataaccg gcattgcgac
    10021 cgagaatggt atacgcacgg gtcatattga tgccgtacag ccagtcggca cgcgctcgcg
    10081 ccagcgcaga aacgcacaat ggcacaaact cactgttgga acgaagacgg tcgatcgccc
    10141 gctcaaccgc ctgcgggttc aggtcgttta tcaagcaacg ctgcacctgc tggcgctttt
    10201 ccggtgccag ttgcagatag tccagcactt catccaccag caattgccct tcacgatccg
    10261 ggtccccggc gtgaacgatt tcgctggctt catgcaggaa gcgtttgatg acgttaagtt
    10321 gtttggtcac ggagggtcgt ggctgtaatt gccacttttc cgggacaatc ggcaaatccg
    10381 caagattcca gcgcgcatag cggctgtcgt aggcgtctgg ctgcgcctgc tcaagcaggt
    10441 gaccgataca ccaagtcacc acctgaccat taccgcactc gataaagcca tcgcctttcc
    10501 ggtgcggttt gggcaggaca tcagcaatgg cgcgcgccag actcggtttt tcggcaataa
    10561 acaaccgcat tgaattaacg gatctcaacc atggcacgac cgccgcgcgc tggcaccagt
    10621 tcgccaatcg ccgtcagttc aatgccaaac tcggcggctg tagttttaac ctcattttct
    10681 gcttccggca tgaccgccag cagcaaaccg ccagaagttt gcggatcgca cagcagatcg
    10741 cgcacttcac gcggcatttc acccatcaga tgaccgtagc tggcaaagtt acgttcagtg
    10801 ccgccaggta ctgcgcccaa cttaatgtac tcttcaacac cggggagttt cgggatcgct
    10861 tcatagtcga cgcgtgcctg cacaccagcc ccctgacaca tttcgctcaa gtggcccagc
    10921 agaccaaagc ccgtaacgtc ggtcatcgct tttacgcctt cgatgttggc aaaggacgcg
    10981 cctgcgatgt tcatccggca catcacttcc gtcgccagtc cctgatgttc tggtttcaac
    11041 agtgattttt tctcagccgt ggtaagaacg ccgatcccca gcggtttcgt caggaacagt
    11101 ttgcatccgg cttgtgcggt actgtttttc ttcacccgct cggtcggtac gatccccgtt
    11161 accgccagac caaaaatcgg ctccggcgca tcgatggagt gaccgccagc cagcgcaata
    11221 cccgcctgac ggcatgcata gcgtccacct tcggttactt cgcgggcaat ttccggggaa
    11281 agtttgttaa tcggccagcc gaggatcgca atcgccataa tcggtttgcc ccccatcgcg
    11341 aagatatcgc tgatggcgtt agtcgccgca atgcggccaa aatcgaaagg attatcaacg
    11401 atcggcataa agaagtcggt ggtactgata acgctggtgc cattgcccag atcgtacacc
    11461 gccgcatcgt cgcgggtttc attacccaca agcaaattcg gatcaacaaa cttcgcctgc
    11521 tcactgtgca ggatggtttc caacactttt ggggaaattt tacagccgca accagctccg
    11581 tggctgtatt gggtcaaacg aatcgagttc tcgctcatgg acatctcctg tcaatgcaat
    11641 ccgggtatgg taaccctcat tccgtgaagt gataagtgag agtgtctgaa ttcctgcgcc
    11701 tttgctcaca atccagacag tttcgcgaca attatcagaa ataagttaca aacggcgtcg
    11761 ggtccgggac gttaatcgac gtagatgctt tcaactgcgg cgtgccgagg tagagaaaac
    11821 cgacaatttt atcctgctca cggcaaccga atgcttcacg cactaccgga ctttcagtta
    11881 atgcgccact gcgccagatg ccgccaaatc cctgggcaac ggctgccatt tgcatcgcca
    11941 tgaccgcgca tccggcagac atttcctgtt cccatcgcgg gactttatga ttttctttgc
    12001 atttcgccac caccgtgatg atgagcggtg cgcggaacgg cgcattacgg gctttgtcga
    12061 tagctttatc atcactaccg gcagcaatcg ccccctgttc cagtacggcg ctgaaacgct
    12121 cgcccccttc cccttcaatc acaaaaaaat gccacggttg catggactta tggtccggcg
    12181 cacgcatacc cgcacgcagg atgttttgca gttgttcacc cgttggcgcg ggttcagcca
    12241 ggcgggaggc gctacggcga ttgatcaata gttcgagtgc atccatttga ttaactcctg
    12301 tcgtgatatt tattcacaaa attaacacga gagtggattt tgttacagca cagtccgcaa
    12361 ttcctgctga caagtgccgg ttgggtcatt acgataacca catctattgc gcctgtgaca
    12421 ggtgtgacct taagttggga gaatacatgc gaaccctttg gcgatttatt gccggatttt
    12481 ttaaatggac gtggcgtctg ctgaatttcg tccgtgaaat ggtacttaac ctgttcttta
    12541 ttttcctcgt actggttggt gtggggatct ggatgcaggt cagtggtggt gattcgaaag
    12601 aaacggccag tcgtggcgca ctgctgctgg acatttctgg tgtgatcgtc gataaaccag
    12661 acagttctca gcggtttagt aaattaagcc gccagctgct tggtgccagt tccgatcgtc
    12721 tgcaggaaaa ctcactgttt gatatcgtca acaccattcg ccaggcgaag gacgaccgca
    12781 atatcaccgg tatcgtgatg gatctgaaaa acttcgcagg cggcgaccag ccgtctatgc
    12841 agtacatcgg caaagccctg aaagagtttc gtgacagcgg gaaaccggtt tatgccgttg
    12901 gcgagaacta cagccagggg caatattatc tcgccagttt cgccaataaa atttggctgt
    12961 caccgcaagg cgtggttgat ctgcacggtt ttgctaccaa cggtctgtac tacaaatcgt
    13021 tgctggataa gctgaaagtt tccccccatg tgttccgcgt gggtacgtat aaatctgccg
    13081 ttgaaccgtt tattcgtgat gatatgtcac cggcagcccg cgaagctgac agccgctgga
    13141 ttggtgagct gtggcaaaac tatctgaata ctgttgccgc taaccggcag atccctgctc
    13201 agcaggtatt ccctggcgcg caagggttgc ttgagggttt aaccaaaacc ggtggcgata
    13261 ccgcgaaata tgcactggaa aacaagctgg tcgatgcact ggcatcgagt gccgaaatcg
    13321 aaaaagctct gaccaaagag ttcggctgga gtaagactga taaaaattat cgcgccatca
    13381 gttattacga ttacgcattg aaaacgccgg cagataccgg tgacagcatc ggtgtcgtct
    13441 tcgctaatgg cgcaattatg gatggcgagg aaactcaggg gaatgttggc ggtgatacca
    13501 ctgcggcaca aatccgcgac gctcgccttg acccgaaagt gaaagcgatt gtcctgcgtg
    13561 ttaatagccc aggcggcagc gttaccgcgt ctgaagtgat tcgcgctgaa ctggcagcag
    13621 cccgggcagc gggtaagtct gtggttgtat cgatgggcgg catggcggca tctggtggtt
    13681 actggatttc cacgccagct aattacattg tggctaaccc cagcaccctg accggttcta
    13741 tcggtatctt cggcgtgatc accaccgtag aaaatagtct ggattcgatt ggtgttcata
    13801 ctgatggtgt ctcaacttca ccgctggcgg atgtttctat caccagggca ctgccgccgg
    13861 aagcgcagca gatgatgcaa ttaagcattg agaatggcta taaacgcttt atcacgctgg
    13921 ttgctgatgc gcgtcattcg acgccggagc aaattgataa aatcgcccag ggccacgtct
    13981 ggaccggtca ggatgcaaaa gctaacgggc tggtcgatag tctcggggat ttcgatgatg
    14041 cggttgccaa agcagcagag ctcgcaaaag tgaaacagtg gcatctggaa tactacgttg
    14101 atgaaccgac cttcttcgac aaagtgatgg acaacatgtc tggttctgtc cgggcaatgt
    14161 tgccagatgc gttccaggcc atgttacctg caccgcttgc ctcggtagcc tctaccgtta
    14221 aaagtgaaag cgacaagctg gccgcgttta acgacccaca aaaccgttat gcgttttgcc
    14281 tgacctgcgc caacgtgcgt taagtcttgt actgagtggc cgacagatcg tcggccacat
    14341 tattttttac ctcgacgaat cctcttcccg ctgtctcgcc ccatatttcc ttatccgcat
    14401 agtatcaggt gcgctcctcc tgcctcacgt atatactttt gctctttcga tatcattcat
    14461 atcaatatca tgcaaaagaa atcaatttac gtcgcctaca cgggcgggac catcgggatg
    14521 cagcgttccg agcagggtta tattccggtg tcaggtcatc tacaacgcca actggcgctg
    14581 atgccggaat tccatcgccc ggagatgcca gatttcacca ttcatgaata tacgccgttg
    14641 atggattctt ctgatatgac gccggaagac tggcagcata ttgctgaaga tattaaagcg
    14701 cactatgacg actatgatgg ttttgtcatt ctacacggta ccgacacgat ggcgtatacc
    14761 gcctctgcgc tgtcgttcat gctcgagaat ctcggtaaac cggtcattgt gacagggtca
    14821 caaatcccgc tggctgagtt acgctctgac ggacaaatta atctgctgaa tgcgttgtac
    14881 gttgcggcga attatccgat caacgaagta acgctctttt tcaataaccg attgtatcgc
    14941 ggcaaccgca ctaccaaagc ccatgccgat ggttttgatg cgtttgcctc tccaaacctt
    15001 cctccgttac tggaagctgg tatccatatt cgtcgtttga atacgccacc cgccccgcac
    15061 ggtgaagggg aattaatcgt tcatccaatc acgccacaac caattggcgt agtgacgatt
    15121 tatccaggga tttctgctga cgtcgtgcgc aattttctgc gccaaccggt gaaagcattg
    15181 attctgcgct cctatggcgt gggtaatgcg tcacaaaaca aagccttcct gcaggaatta
    15241 caagaagcca gcgatcgcgg tattgtggtg gtcaacctga cacaatgtat gtccggtaaa
    15301 gtgaatatga gtggttatgc caccggtaac gccctcgccc atgccggcgt tattggcggt
    15361 gcagatatga ctgtagaagc cacgctaacc aaactgcatt acctgctgag ccaggaactg
    15421 gatactgaaa ccattcgcaa ggccatgagc caaaacctgc gcggcgaact gacgccggat
    15481 gattaaggag actgcaatgc cccatcgcgc cctgttactg gtcgatttac aaaatgattt
    15541 ctgtgctggt ggcgcgctcg ccgtaccgga aggtgacagt acggtggagg ttgctaaccg
    15601 ccttattgac tggtgccagt cgcgcggaga agcggttatc gccagccagg actggcatcc
    15661 ggcgaatcac ggcagctttg ccagccagca cggcgtagag ccttatacgc caggccaact
    15721 cgacggcctg cggcaaactt tctggcctga acactgtgtg cagaacagtg aaggcgcaca
    15781 attacatccg ttactgaatc aaaaagagat cgcagcggtg ttccataaag gcgaaaatcc
    15841 tttagttgac agttacagtg ccttttttga taacggacgt cggcagaaaa ccgcgcttga
    15901 tgactggttg cgcgatcatg aaatcgatga attgatcgtt atgggcctgg ctactgacta
    15961 ttgcgtgaag tttaccgtgc tggacgcgtt acagttaggt tataaggtaa acgtgattac
    16021 cgatggctgt cgtggcgtga atatccagcc ccaggacagt gcacacgcgt ttatggagat
    16081 gtcagcagct ggggcaacgc tatatacgct ggcagactgg gaagagacgc aggggtaatt
    16141 ttacgctggc ctacaattct gtactggcat tgtaggccaa ataaaacacg tcagtggtac
    16201 atctggcaat tgatgccatc aacgaaagat ttaattcacc tcagaaatct cttccagcga
    16261 caccttccgc gtttcgatgc caaaaatgga gagaaccagc gcacagagca ctaacatgac
    16321 accaagcacc ataaacaccg tgatcgaccc ataatgtgtt aataatgccg caacgccata
    16381 gggcgtgaaa actgcgacga tccgtccgac ggcattaacg aaacccgaac cgcgcaggcg
    16441 taaatgcgtt ggccaaagct ccgggatata aaccgccgac gcgaagcaaa catacatgta
    16501 taaaaagaag atcatcacca gaccatagat taaaatcgcc cactctgtag tctgacttga
    16561 atagatatag cctaacacgg caataataat aagtaaggcg gagccaaata atcgacgagg
    16621 aaaatgatca ataattaatg ccgcaataaa tattcctacc ggagcaccaa tcataataac
    16681 agcggtcatt aatattgatt tatcgacatc aatgccggag ttaacaaata tggtcggtat
    16741 ccatacggta atggtataaa gcgaaatgtt cattgcaatt aaaacagtta tcgcaactaa
    16801 tgtacgtcgt aacatttccc ctttaaacag gagccagaag gtacccttga ttaccttaac
    16861 cttgctgttg ctctggttca aagtaagctg gggtaaacga atactcttct ccctttcaat
    16921 ttgctgctct acttcacgaa gttggctttc tgcaccggcg atttgccctt tccctgccag
    16981 ccatcgtggc gactcgataa agtatttacc tgagagaaac caggctaaca gtatgccaat
    17041 accacccaga agaaacatta ttcgccaact aaaaaaagcg ataaccacca cgcctatcgc
    17101 cgcagacagc atgggcgacc agttaccaac aaatgagagc cgcgcggacc attttccacg
    17161 caccgtcgcg gggataaact ccgtaaatga ggcatagcca accataatca gcgcccccat
    17221 tcctgttccc atcaggaagc gaaagaaaat gagccagtac atatcaggga caaaagcggc
    17281 ccctgttgca gcaataccga cgatgagaag atttatgcga aacgccctgc gccgcccaaa
    17341 gtagtcacca ataaacccac cagtaagtga gccgatgaaa taaccaaaca ttaatgccga
    17401 ggtaaaggcg gcattgagga aattatttga ccatccattg cttaccagct ttgctaagac
    17461 gacattacct gagtaactca aaaaccccgt taataacaga ctaaagctta ttataccaaa
    17521 tatacgataa tgaaaccggg ccaaaggcaa gcggtccagt cttgcgccaa tttgatcata
    17581 ttgttccatt gggtgcctcg gatttgctaa aaaaggtagc aattgcggca aattcacttg
    17641 ccgcatcttt atcatcattt tatttccagg cgcaggaaca aataatattg acaacgtgtg
    17701 cttaaattac cacaccagct ggatattatt gtctttgcaa aatgctatcc actctgcacc
    17761 cggtgattta tcagttatta tataattaat atgactaaag tcagctaact ggacaaaagc
    17821 tttacgatca aacttagagt gatcaaccaa taacgcaact tctgtcgcct gacggatcat
    17881 tgtctttttg atttcagctt ccgcttcatt agagtccagc gcgccgctgt taatatcaag
    17941 acctttacaa ctcattacca tgatatcgac atggtagcgg ctgatgatct ctttagtaat
    18001 tcttccttgc agggaaagtg tatttttgtt tagttcccca cccgttgaaa cgactttaat
    18061 ttctgactga gccagaacat gaatcgcttc cgccgagttg gttagcaacg tcaggtcact
    18121 acggtcctgt aacaatttga gcaattccat tacggtacta ctggaatcgg ctgccatggt
    18181 ggttttattg tcgataaagg gtagtgcctt gcgtgcaata agctgcttct cttcataaaa
    18241 cgatgaagcg cgcttataaa aatggatatt ctccgtcaac atcgctgtat ttaaaacagc
    18301 accaccatag gttctggtca aaaagccttc atcttccagc ttctcaagat cgcggcgaat
    18361 ggtttcttcg gttacctgaa aaatcccact caaatttgag actgtcacct ttttatcgtt
    18421 ggcaaccatt tgcttaattg cctgaatcct gtcttttgcc gccacgatta cacccctgta
    18481 tctttttaca tcacattagc gcgattatcg cataaccgat gtttactttc aaaataacct
    18541 atttgaatca cagattttca tcacagtttt cacagaaaca gaggtgaatc gtgttgagta
    18601 tttaacgctc cagggcctct gccatttccc tcatcaatgt tgcgtctgca tccgataagt
    18661 tgatattcag tgccgcgaca ttttcgcgta cctgttccgg tgcagtagcc ccactaagaa
    18721 tggagattaa atcactctgt tttaatatcc acgccagtgc cagagtggga attgtgcact
    18781 gataacgagc acaaagtggc tgccactgtt caagcatatc aatcaatttc agcatgtttt
    18841 cacgctggaa ccagacttta tttgcccgag cgccgcccgg aacgtaatca cgagtgatgg
    18901 tgccggtcaa caatccctgc tctagcgggg aataaacctg aaccacaatg ccattatcac
    18961 gacatagcgg cagcagttcg ttttccattg cccggtcgag aatactgtat ttcgcctgaa
    19021 taatatccag ttcaccatat tgcagatact cgcggatatg gtcagcatcg acgttagcag
    19081 cgcctatagc gcgaattttc ccttcagcct ttaactcatt aagcactgcg acagtttcag
    19141 ctatcggcgt aaaaaatggc ggcaccgact gccagtgcgt catatagata tcgatgtaat
    19201 caatacccag acgttgcaag ctggcttcta cctcttcgcg gatagattcc ggggaaaggt
    19261 ttttatacaa ctgccgatcg ccaactttgt tgaataaact tccttttcgt tcccagacaa
    19321 tgccgcattt ggtttctact acaacctgtt cacggggcag tttttttaac gcctgaccga
    19381 cgataacttc actattgcca aagttatatc ctggcgcagt atcaatcaga ttaatgccac
    19441 aacgatgggc ttcaagaatc gtatcaatac atatttgccg atcgagatcg ccattccatg
    19501 caggaccgcc gccaatggcc catgtcccca accccattcg cgaaagcgtt atatccgttg
    19561 tgcctaaagg tatctttttc atttgccttc attatccttc gtattcttcc agcaattgtt
    19621 cgaccagctt tctgtttttt acgccggtgg tggcaccgac gcttagaacc gagatagccg
    19681 ccgttgcatt ggcaaagcgt gcgcattcac gcagattttt gccttctaac agtgccgcaa
    19741 taaaacctga agcaaagtta tcgcccgcgc caatggtgtc gatggcggtt attcctgcga
    19801 ccgccggcac cttcatcgtc atgtcaccac gcttgataaa gcagccgtct ttacccgttt
    19861 taatcaccac cgtttttacg ccgcacgcaa gaaagcagtc agcaatttca tccagtgtct
    19921 ctttccctgt gagtaatttt gcctcggcaa aatttggaaa cagataatcg acataactca
    19981 atgcttcgca aatatcatcc agcgtttcat tcaaccgcgg tttgatcata tcggcacaaa
    20041 tgatcatctg ccgggctttc gcttgcgtaa aaatttctgt tagcgcttta ccatccaata
    20101 gaggactgtt gaaaatactg gccagtgata ataatttcgc ctgaggaaac cgcgcaaaat
    20161 caacatcgtc aatatttaat ttccacaaac tgccgttacg gttcgtgaca aacgtccgct
    20221 cgccatcctc cgtcaccagt ccaacgttaa tagatgtatc tatgctgacg tcctgtttca
    20281 ggctttgaat atcaatattc tctttgcgac aatggtcgag aataaattgc cctgaggcat
    20341 ctttaccaat gcggctcatt aatgcggtac gatgccccag acgagaaata attgttgctt
    20401 catttattgc atcgcccccg gtgctcattg cgattctttc aagaggataa gaatctacat
    20461 caaagatatt tttactgacc ggttgtaatg gaatatcaac aatagcggca cctatacaaa
    20521 taacgtcgag attatccata tcattattcc gctttgccat cagaaccgaa cagttcaatt
    20581 ttttccaatg cccgttcttt tacagcttta cgtacttcac gttctaaatg caggaaaggc
    20641 tgatcctggt tttctttcac tgctaccatt gcggcctgac agagctccgt atgaatgttg
    20701 attttcgcaa tacccaacga aattgcagtt ttaatgtcgg catcactgat ccccgatgcg
    20761 ccatgcaaaa ccagcggaac agaaacagca tcgcgtacgc gtttgacgac ctcaaagttc
    20821 aattgcggct ctgacgtata aaccccatgc tggttgccga tggcgacagc caatgagtca
    20881 cagcccgtac gttcaacaaa ttcagccgcc tggtcaggat cggtatagtg atagcccgcc
    20941 agcgcttcct cataaacggt ttcattaccg acatgcccta attccgcctc taccggaata
    21001 cccagcggat gaaagaaatc aacagcctct ttggttaaac gaatattttc ttcgaaatca
    21061 aatgcagaag catcacgcat taatgaattc ataccatgag tccaggcgtt atgaataatc
    21121 tccatactcc gaccatgatc ccaatgagtt attaccggca ccgttgcttt ttgtgccatt
    21181 gataccatca tgtgagagaa atcttcaaat gaggtgttac cgacaaaacc tgtaccaaaa
    21241 gaaataataa ccggggattt cgcttcttcg gctgcgtctt taacgcccat caacatttct
    21301 gcattccata cgttaaaatg ggcaattgca taatgtttat ttgtggcatc gttttcccaa
    21361 tatctgatat ctgcgagcat gttctgttct cccgtattaa ttaatcgcta attttaataa
    21421 cgcctttaat aatgtcgcgt ttgttgttaa ctgactcttc aaatgcctgt tgtacatccc
    21481 gataatcgta aatatgcgtc accatcgatt tcacatcgaa tcgccctgaa gaaatagctt
    21541 caatcgtgac cggataacga ttggcatagc ggaataccgt ctggatagtg acttcgcgat
    21601 tgattttgag gaaattgatt gccgaatcgc cgggtacagt accaacaatc ataattttac
    21661 cgccgcgcat taccagataa ggtgcctgtt taacggtgac cgcagaaccc gctgtttcga
    21721 aaacaatatc tgcgcccatg tcttcggtaa attgctgaca gcgtgcaata gtgtcctctt
    21781 ttgcgccatt aataaccact gtcgcaccaa gctgttccgc cattgccaga cgtttttcca
    21841 gtacatcaac gacggcaatt tccgttgcgc ccaggcattt gcacgcttgc aacgtcatca
    21901 aaccaataca acctgctccc agaataatta tcttcttacc cggtttaaca tctgccagca
    21961 tcgcggcatg catcccgact gcggcaggct ccaccagcgc cccttccatc gtgtccatat
    22021 tgtcgggcag tttgtaagta aagctctccg gatgacacag atagtgcgtt aatgcgccgc
    22081 ggtagttggg ttgtgtcgcc ataaaatcaa cgtccgggca gatgttatat ttgccttcca
    22141 gacagtaacg acagtgaccg caaggaacgc caggttcgat atttacccga tcccccggtt
    22201 taaatttgcg cacgcggctt cccacagcca caaccgtccc ggcgcattca tgacccaggc
    22261 caatttcttg atttgggtct ttaggcggaa taaacgggcc tgattcaaaa ccatgtacat
    22321 ctgaaccaca aataccgaca tattctactt taatcaaaac ttcatcttct ttaggcactg
    22381 gtatttctgc tgaaataatt ttcattgtgc ccggcacctg caatattgct tttgaatttt
    22441 tcattttgaa tccacccgta ttatttattg gctactgcat caatacttaa actttcaacg
    22501 gagacacctt tggtttcaat tccaatggtg gcgatagcaa ttgcgacaat aattgaaaca
    22561 gcccccagaa gaataaagac tcccgttaca ccataactac tgagcagcac tgcaacggca
    22621 taaggtgcgg caataccact gattcgccct accgcattcg ccagaccgga gccacggagt
    22681 ttggcctctg tcggccagat ttcagggaca tacactgccg aggcatagca aacgtacata
    22741 tagacgaaag taatcaggaa gaaaccaatc agcgttatga gcaacatact ggtttgcagt
    22801 gaatagatat atccgagcac cgcaatcagg attaatagcc ccacacccat tgttttacgc
    22861 ggaattttat ccatcaccag catggcaata aaaataccaa atggcgcgcc aaacatactc
    22921 atggtattta aaacaatcga gtcttttaaa ttaatcccct gggtcatgaa tattgttggc
    22981 aaccagttaa ttagtgtgta ctgcacaacg ttcatggtaa tcagcacaca agagcctaat
    23041 atcacgcgtt tcagtaatac tcctgtcagt aaggctgaat acggcaccgc ttgtggcgct
    23101 ttaccgtcat cagcaataac cacaggcggc aaaggtttac ccgtctggcg tatgacgcct
    23161 tcttctatac tccgcatgac tttctcggct tcctgatacc gtccgcgcga ttccagccag
    23221 cgcggggatt caggaaagta gcgccaggcc agcgccgtag cgataagcga caatattgca
    23281 ggaataagca gttgtacccg ccagttccac tctgcactaa tcagcggcgt gagtcccatc
    23341 gcaatcagtg agcagagcgg atatgaccag ttgccaataa aggaaacacg actggaccac
    23401 gttccgcgat ttctaccggg catatattcg gtgaaaccag caagcagtgt aaccagtaaa
    23461 gctcccagcc caacgcccat cacaaaacgg caggcaataa gaaaatccat attcggtgag
    23521 aaagcaccga caaccatcga ggcaatatga atggcctcat agaggatgaa cgcatttctg
    23581 cgaccggttt tgtcgccaat gatgccgccc accagcgcac cgagaaacat tccggcggtc
    23641 gtgattgctg agaatgtggc tgtggtggaa ttatctgtcc agcccaacgc tttcagctgc
    23701 gcgaggatca agccaccaac ggcattactc cagcagacaa gcaagccaaa cgcgacgatg
    23761 gcaaacattg acgaatgcca gcggcaatcc ggtaagcgat ccagccgtgc accacaatgc
    23821 ggttttgtta tctgttccat gcgataacgt tccttcagga gagtagttat tcatcaaagt
    23881 cgtaagtcat gatcactttg attgcggttt tatcgaccat cgcatcaaac ccttcgcgcc
    23941 attgcgacag gccgatacga tgcgtgatca tcggtttgac tttgatagcg ccgctggcta
    24001 atagccggat agcgttacgc catgaggtgg agtcataggc catatgccca atgatgcttt
    24061 tattccaggc ggtaatgtca ttaatcgaga aatcaagagg tttgaagccc attccaacgc
    24121 gtaccacttc cccattcggg cggagcattt cgatggcttg tttcagtgcg atattggcac
    24181 tggagcattc aatcaccagt cccagattat ctttgccaca aatttgctgg cagcgcgcca
    24241 ccacatcttc ggtagaacca tttactactg ccgtcgcccc cagttctttt gcaaccggga
    24301 agcggaccgc cacatcttct tgcagaccaa cgacgacgat atttaccgcc cccataatcc
    24361 gcgccatttg tacggaaaac agcccgagtg ggccagtgcc aatgacgaca acatcctgac
    24421 caggaaggaa tttcgattgc tgcgcgatgg atttataggc attacagata gggtcaagta
    24481 cggctgcgtc ctcataatca acaccatcag ggatttccca caacgcatga cgatgaattt
    24541 tgagaatttc accaggaacc agacaatatt tggaaaaacc accgccccag gtattgttat
    24601 ccagaccaag gtttactttt tctgtacaac acagaaaatc accctgttca caggccggac
    24661 aaacgccgca aacgtgaccg ctgttatccg acacgacgcg ttgccccact ttccagtctt
    24721 tgactttttc accaacctgc gcaatacaac ctgcgaactc atggccgcgg atagagttaa
    24781 actcatcaga accgctatcg acattgtagt gcttcatgtc tgcgccgcag attgccgcgg
    24841 ctttaatttc aatcactacg tcttccgggc cacaaatggg ttgtgggaca tgaatcatct
    24901 tgtagccgcc aaaggccttg ccaaaccgag ccagtgcttt cattgcgtca tcctctgtgt
    24961 tttgttttgc ttagatggcc atcatactta cccacatgat ttttgaggtc aacattaaat
    25021 ctgcataaca cagataatga cctgcgcttt acaacttaga tcacaattac gcagaaaatg
    25081 gctgggtctg ttacaggttg gtggaaggcg ggacgcaaaa agagcaaatt cgagagggat
    25141 taccggccaa ataggtctga gacttaagtt caggagaagt cattcaggcg tgaacgcgac
    25201 tgcaagagat cggactggga cttagccagg tcgggtcgag ataattgaga cgagtcgtgg
    25261 ggtcattgtt ggccatcccc ttcatagccg cggcaaaaag gggatgaacc acttacttaa
    25321 acatggcaat aggctttgct gagataccaa aatcttcttt taactgctgt ttgctcttca
    25381 tgaccatctg accatttgtg tcaattgtca tatgctgtgc ttcggtatta tggcgggctt
    25441 gccacagcat caccagttgc aggcaattct ctttttgttc ctcggtcaac gcaacgccat
    25501 caggccattt ccccagttca acggcggtcg acaaacgctg gtatacctca ggcgtcatgc
    25561 tgttgataat gtcatcaaga ttcataattt ttccgctcct gtggaataag ttgctgaatc
    25621 gttttttcaa ccgttgattt cttcgccgtt ttcgccatca gtgaagcgta aagaagcaga
    25681 gttaacacaa taacgttcgc ctgttggttg cggcccgtcg ggaaagacat gccccagatg
    25741 ggcatcacag ttaccgcaac gaatttctat gcgctgcatt ccatgtgaca agtctttgat
    25801 ataacgaatg gattcttcac tcaccggttc gtagaaactg ggccagccac agccggaatc
    25861 atacttggtt tcggaatgaa acagcggggc atcgcagatc aaacagtgat atacgccgtc
    25921 acgcttgtta tgcagtaaac gacccgtaaa tggcggttct gtcccatgat tctgcgtcac
    25981 gtaaaactgc atctcggaca aatttttttt cagttcttct gccgaaggtt tattagccat
    26041 ttactcacat ctcactttaa tcgtgctcac attacgtgac tgattctaac aaaacattaa
    26101 caccaactgg caaaattttg tcctaaactt gatctcgacg aaatggctgc acctaaatcg
    26161 tgatgaaaat cacattttta tcgtaattgc cctttaaaat tcggggcgcc gaccccatgt
    26221 ggtctcaagc ccaaaggaag agtgaggcga gtcagtcgcg taatgcttag gcacaggatt
    26281 gatttgtcgc aatgattgac acgattccgc ttgacgctgc gtaaggtttt tgtaatttta
    26341 caggcaacct tttattcact aacaaatagc tggtggaata tatgactatc aaagtaggta
    26401 tcaacggttt tggccgtatc ggtcgcattg ttttccgtgc tgctcagaaa cgttctgaca
    26461 tcgagatcgt tgcaatcaac gacctgttag acgctgatta catggcatac atgctgaaat
    26521 atgactccac tcacggccgt ttcgacggta ccgttgaagt gaaagacggt catctgatcg
    26581 ttaacggtaa aaaaatccgt gttaccgctg aacgtgatcc ggctaacctg aaatgggacg
    26641 aagtgggtgt tgacgttgtc gctgaagcaa ctggtctgtt cctgactgac gaaactgctc
    26701 gtaaacacat caccgctggt gcgaagaaag tggttatgac tggtccgtct aaagacaaca
    26761 ctccgatgtt cgttaaaggc gctaacttcg acaaatatgc tggccaggac atcgtttcca
    26821 acgcttcctg caccaccaac tgcctggctc cgctggctaa agttatcaac gataacttcg
    26881 gcatcatcga aggtctgatg accaccgttc acgctactac cgctactcag aaaaccgttg
    26941 atggcccgtc tcacaaagac tggcgcggcg gccgcggcgc ttcccagaac atcatcccgt
    27001 cctctaccgg tgctgctaaa gctgtaggta aagtactgcc agaactgaat ggcaaactga
    27061 ctggtatggc gttccgcgtt ccgaccccga acgtatccgt agttgacctg accgttcgtc
    27121 tggaaaaagc tgcaacttac gagcagatca aagctgccgt taaagctgct gctgaaggcg
    27181 aaatgaaagg cgttctgggc tacaccgaag atgacgtagt atctaccgat ttcaacggcg
    27241 aagtttgcac ttccgtgttc gatgctaaag ctggtatcgc tctgaacgac aacttcgtga
    27301 aactggtatc ctggtacgac aacgaaaccg gttactccaa caaagttctg gacctgatcg
    27361 ctcacatctc caaataagtt gagatgacac tgtgatctaa aaagagcgac ttcggtcgct
    27421 ctttttttta cctgataaaa tgaagttaaa ggactgcatc atgattaaga aaatttttgc
    27481 ccttccggtc atcgaacaaa tctcccctgt cctctcccgt cgtaaactgg atgaactgga
    27541 cctcattgtg gtcgatcatc cccaggtaaa agcctctttt gcattacagg gcgcacacct
    27601 tctctcgtgg aaacctgcgg gtgaagaaga agttctgtgg ttgagcaaca acacaccgtt
    27661 caaaaatggc gtcgctattc gcggtggcgt accggtttgc tggccgtggt ttggtccggc
    27721 ggcacaacaa ggtctgcctg cgcacggttt tgcccgcaac ctgccgtgga cgctgaaatc
    27781 acatcatgaa gatgctgatg gcgtagcgct gacttttgaa ttgacacaaa gcgaagagac
    27841 gaaaaaattc tggccgcacg actttacgct gttagcgcat ttccgcgtgg gtaaaacctg
    27901 tgaaatcgat cttgaatcac atggcgaatt tgaaaccacc tctgccctgc atacctactt
    27961 taacgtgggt gatatcgcta aggtaagcgt cagtgggctg ggcgatcgct tcattgataa
    28021 agtgaatgac gcgaaagaag atgtactgac cgacggtatt cagaccttcc ctgaccgtac
    28081 cgatcgcgtg tatctgaatc cgcaagattg cagcgtgatt aatgatgaag cgctgaatcg
    28141 tattatcgcc gtaggccacc agcatcatct gaacgttgtc ggctggaacc cgggaccggc
    28201 gctttcagtt agcatgggcg acatgccgga cgatggctac aagacctttg tttgtgtaga
    28261 aacggcttac gcttcagaaa cgcaaaaagt caccaaagag aaacctgcac atctggcgca
    28321 atccattcgc gttgccaaac gttaatttgc gttaatgttg tgtgccgggt gcgatgcatc
    28381 cggcacgtat caacatcaca ccatatccag cgcagttttt ccttttggtg ccggatatgc
    28441 cttatccagc atagctaatt ccgctgacga aagttcgacc tcaagcgcag ctgcattttg
    28501 ttggacatgt gcaatttcgt tgactaccgc gttttttaac agtccattgc gcaaccgccc
    28561 ggcctgggct aatggactgt aagccatcac cggcatctgc tgttgctggc accaggggag
    28621 tagatcgtac tcaatccctc gtgaaccgag atggtaaagc acctgattag tggcacactg
    28681 atttcccccc ggcagctgcc agagttcctg catatcggca taaccaaggt tagaaacgcc
    28741 ccagcgacgg atttttccct gggcgatcaa tttttccatc gcagcaacag tctcttcaaa
    28801 agcgaaactg ccagaccagt gtaataagta gagatcgaga taatcagtat tgagacggcg
    28861 taaactggct tcgcatgcat ttatcgcttt ttgcccgcca gcattccacg gatagacttt
    28921 agagacgaga aagaccttct ctcgcagacc ggttaatgct tccccaacca ccttttcggc
    28981 accgccatcg gcatacattt cggcggtatc aatgagggtt aaaccgagtt caatgcctgc
    29041 gcgtagtgca gcaacttctg ttttgcgctg actggcatct tcgcccatat accatgttcc
    29101 ctgccctatg gctggcagtg agacatcgcc actaaattga atcatttttt gttgcattgt
    29161 ttcctcccag gtattgcacc accgtaatgc aaaacagggc gtgacgccct gtttttatgc
    29221 acaaaatgcc ctggaaagat gcattatcag aatttgtagg tgatcccggt agaaatcagg
    29281 ccagtccagg atttatccac catcgggctg tcagtaactt catcagacag acgggtgtag
    29341 cgcgcggtac cgtaaacact ccagtcgccg aggaagttgt agctggcgct cagctcaagg
    29401 taagggctcc agctgtcatt cgggttatag ccacgcagac cgctgcgagc ggactctttg
    29461 cgcgatacgc cataatagta ttcgttctgg ttttcgctgt tccactgcac accaataccc
    29521 ggagtcacgg tcaggccacc gttggtgtaa cgatacaacc aggccatatc ccagacaatg
    29581 ccgttgctgt tatccagggt atcgccagcc agggtggtac gcaggtaacc gtactgggta
    29641 aagtgagcat aagacagacc agccatcatg gtgctcttac ggtcatccag gtgacgcatt
    29701 tggtgatcgc cactgtcttt cgctttgaag taaagcggcg accagtaagc ggtaattgaa
    29761 agtttatccg ttgcgtcatt ccacaggtag taaccaccac ctaagccacg gaaccagaag
    29821 ttatcgcctt catagttgat taccggtact gggtaaacat cggtatcgta atctttatat
    29881 gggtgctcaa cgacacctac gcctgcgccc agggaaaatt taccttcagc gtgcgctacg
    29941 cctgcagacg ttgcgataag cactccaagt gccagaagtt tgagtttggt cacaattaat
    30001 cattccttaa acaaatgttt agcgggcgac aaagtttacc cgtcaataca tcgaaaccca
    30061 acctttttac gttttcattt tttaaagtaa ctgtttaatt ttcctgccgc ggatgacacc
    30121 gcgcttacag ccaaatgaat ttagcgttac tggcgagcct ggtctttaca ttaattatgc
    30181 aaaatttatg gatgagttgt tgatatgcca ttgaaattaa gaaagccgtg caggcaagtt
    30241 ttccatttgc catctacgct taattttgaa ggtgtatcac cgggcacgtt gttctcatcg
    30301 tcgataaaat ggcatgagag ttgctgtgtt ttagcaagag acgtcgttca gtttacctct
    30361 tccgggagcc tctactattc atatgaacgg ctcttaacct gtgctaaaaa acgaaaggac
    30421 ggcataccat gaatatattc gatcactatc gccagcgata tgaagctgcc aaggacgaag
    30481 agttcacact gcaggagttt cttaccactt gtcggcaaga tcgcagtgct tatgccaacg
    30541 cggctgagcg gctattgatg gctatcggtg agcctgtcat ggtcgataca gcccaggaac
    30601 ccagactttc tcgactcttt tctaaccggg tcattgcacg ttatccggca tttgaagagt
    30661 tttacggcat ggaagacgcg attgaacaga ttgtctctta tctgaaacac gcggctcagg
    30721 ggctggaaga gaagaaacaa atcctgtatc tgctggggcc tgtgggtggg ggtaaatcat
    30781 cgcttgctga gcgactgaaa tcattaatgc agctcgtacc gatttatgta ttgagcgcca
    30841 acggtgagcg tagcccggtc aacgatcatc cgttctgtct tttcaatccg caggaagatg
    30901 ctcagattct ggaaaaagag tatggcattc ctcgccgtta tctcggcacc atcatgtcgc
    30961 catgggcggc aaaacgcctg catgaatttg gtggcgatat cactaagttc cgggtagtga
    31021 aggtctggcc gtcaattctg caacaaattg ctatcgccaa aacggaaccc ggtgatgaga
    31081 acaaccagga catctccgcg ctggttggga aagtcgatat tcgtaaactc gaacactacg
    31141 cgcagaatga cccggacgcc tacggctatt ccggtgcgct gtgccgcgcc aatcagggga
    31201 tcatggaatt cgttgagatg tttaaagcac cgataaaagt gctgcatccc ttgttaaccg
    31261 ccactcagga aggtaactac aacgggacgg aaggtatctc cgccctgccg ttcaacggga
    31321 ttattctcgc ccactcgaac gagtccgaat gggtcacttt ccgtaataac aaaaacaacg
    31381 aagccttcct cgaccgtgtt tacatcgtga aggtgccgta ttgcttgcgc atttccgaag
    31441 agatcaaaat ctacgagaaa ttgcttaatc acagtgaatt gactcacgcc ccatgcgccc
    31501 ctggcacgct cgaaacactg tcacgttttt ccattctttc gcgcctgaaa gagccagaaa
    31561 actccagcat ttattcaaag atgcgggttt atgatggcga aagtctgaaa gacaccgatc
    31621 cgaaagccaa gtcgtatcag gaatatcgtg actacgccgg tgtcgatgaa gggatgaacg
    31681 gtctgtcgac gcgttttgcg tttaagatcc tctcccgcgt gttcaacttc gatcatgtag
    31741 aagtggcggc aaacccggtc catctgttct acgtcctgga acagcagatc gagcgcgagc
    31801 agttcccgca agagcaggca gaacgctatc tggagttcct gaaaggttat ctgatcccga
    31861 aatatgccga gtttatcggc aaagagatcc agacggccta ccttgaatcc tattccgaat
    31921 atgggcaaaa cattttcgac cgttatgtta cctacgcgga tttctggatt caggatcagg
    31981 agtatcgcga tccggatacc gggcagctgt ttgaccgcga gtctcttaac gccgagctgg
    32041 agaaaatcga gaaaccggcg gggatcagta atccaaaaga tttccgcaac gagattgtta
    32101 acttcgtact gcgcgccaga gcgaataaca gcggacgcaa tccgaactgg accagctatg
    32161 aaaaactgcg cacggttatc gagaagaaaa tgttctccaa taccgaggag ctgttgccgg
    32221 ttatctcgtt taacgccaaa acgtcaaccg acgagcagaa gaaacacgac gactttgtcg
    32281 accgtatgat ggaaaaa
//
LOCUS       DADLSA010000033        31553 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.033, whole genome shotgun sequence.
ACCESSION   DADLSA010000033 DADLSA010000000
VERSION     DADLSA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.033"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..170)
                     /locus_tag="JN691_001537"
     CDS             complement(<1..170)
                     /locus_tag="JN691_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7243011.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTV"
     gene            383..1846
                     /gene="bepA"
                     /locus_tag="JN691_001538"
     CDS             383..1846
                     /gene="bepA"
                     /locus_tag="JN691_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAY7243012.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKSYTKM"
     gene            1867..2226
                     /gene="arsC"
                     /locus_tag="JN691_001539"
     CDS             1867..2226
                     /gene="arsC"
                     /locus_tag="JN691_001539"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAY7243013.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2359..3105)
                     /gene="hda"
                     /locus_tag="JN691_001540"
     CDS             complement(2359..3105)
                     /gene="hda"
                     /locus_tag="JN691_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAY7243014.1"
                     /translation="MVNFSRFCEILVEVSLNTSAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(3155..4444)
                     /gene="uraA"
                     /locus_tag="JN691_001541"
     CDS             complement(3155..4444)
                     /gene="uraA"
                     /locus_tag="JN691_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAY7243015.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(4530..5156)
                     /gene="upp"
                     /locus_tag="JN691_001542"
     CDS             complement(4530..5156)
                     /gene="upp"
                     /locus_tag="JN691_001542"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAY7243016.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            5480..6517
                     /gene="purM"
                     /locus_tag="JN691_001543"
     CDS             5480..6517
                     /gene="purM"
                     /locus_tag="JN691_001543"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAY7243017.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKCHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            6517..7155
                     /gene="purN"
                     /locus_tag="JN691_001544"
     CDS             6517..7155
                     /gene="purN"
                     /locus_tag="JN691_001544"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAY7243018.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            7327..9393
                     /gene="ppk1"
                     /locus_tag="JN691_001545"
     CDS             7327..9393
                     /gene="ppk1"
                     /locus_tag="JN691_001545"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAY7243019.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            9398..10939
                     /gene="ppx"
                     /locus_tag="JN691_001546"
     CDS             9398..10939
                     /gene="ppx"
                     /locus_tag="JN691_001546"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAY7243020.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEDAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(10978..13221)
                     /gene="pdeF"
                     /locus_tag="JN691_001547"
     CDS             complement(10978..13221)
                     /gene="pdeF"
                     /locus_tag="JN691_001547"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY7243021.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSLIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRCGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(13403..13555)
                     /locus_tag="JN691_001548"
     CDS             complement(13403..13555)
                     /locus_tag="JN691_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243022.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            13573..13764
                     /locus_tag="JN691_001549"
     CDS             13573..13764
                     /locus_tag="JN691_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HAY7243023.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            14075..14593
                     /locus_tag="JN691_001550"
     CDS             14075..14593
                     /locus_tag="JN691_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY7243024.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            14609..15148
                     /locus_tag="JN691_001551"
     CDS             14609..15148
                     /locus_tag="JN691_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243025.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(15243..16820)
                     /gene="guaA"
                     /locus_tag="JN691_001552"
     CDS             complement(15243..16820)
                     /gene="guaA"
                     /locus_tag="JN691_001552"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY7243026.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16889..18355)
                     /gene="guaB"
                     /locus_tag="JN691_001553"
     CDS             complement(16889..18355)
                     /gene="guaB"
                     /locus_tag="JN691_001553"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAY7243027.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            18517..19887
                     /gene="xseA"
                     /locus_tag="JN691_001554"
     CDS             18517..19887
                     /gene="xseA"
                     /locus_tag="JN691_001554"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAY7243028.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(19884..20099)
                     /locus_tag="JN691_001555"
     CDS             complement(19884..20099)
                     /locus_tag="JN691_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243029.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(20169..21641)
                     /gene="der"
                     /locus_tag="JN691_001556"
     CDS             complement(20169..21641)
                     /gene="der"
                     /locus_tag="JN691_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAY7243030.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(21759..22937)
                     /gene="bamB"
                     /locus_tag="JN691_001557"
     CDS             complement(21759..22937)
                     /gene="bamB"
                     /locus_tag="JN691_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAY7243031.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(22948..23568)
                     /locus_tag="JN691_001558"
     CDS             complement(22948..23568)
                     /locus_tag="JN691_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAY7243032.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(23586..24860)
                     /gene="hisS"
                     /locus_tag="JN691_001559"
     CDS             complement(23586..24860)
                     /gene="hisS"
                     /locus_tag="JN691_001559"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAY7243033.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKQLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(24971..26089)
                     /gene="ispG"
                     /locus_tag="JN691_001560"
                     /gene_synonym="gcpE"
     CDS             complement(24971..26089)
                     /gene="ispG"
                     /locus_tag="JN691_001560"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAY7243034.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(26116..27129)
                     /gene="rodZ"
                     /locus_tag="JN691_001561"
     CDS             complement(26116..27129)
                     /gene="rodZ"
                     /locus_tag="JN691_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAY7243035.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(27414..28568)
                     /gene="trmG/rlmN"
                     /locus_tag="JN691_001562"
     CDS             complement(27414..28568)
                     /gene="trmG/rlmN"
                     /locus_tag="JN691_001562"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAY7243036.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(28718..29149)
                     /gene="ndk"
                     /locus_tag="JN691_001563"
     CDS             complement(28718..29149)
                     /gene="ndk"
                     /locus_tag="JN691_001563"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAY7243037.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(29298..31421)
                     /gene="pbpC"
                     /locus_tag="JN691_001564"
                     /pseudo
     CDS             complement(29298..31421)
                     /gene="pbpC"
                     /locus_tag="JN691_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708358.1"
                     /note="penicillin-binding protein 1C; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            complement(31423..>31553)
                     /locus_tag="JN691_001565"
     CDS             complement(31423..>31553)
                     /locus_tag="JN691_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY7243038.1"
                     /translation="YYTRKMSQGKRHNQVLIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT         7950 a         8383 c         7800 g         7420 t
ORIGIN      
        1 acggttggcc attccagcaa ataagccagc acaatagcca ccagcaacgg agcaagcagg
       61 ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa ggcaatcgct
      121 tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat aaagaccttc
      181 cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac aaaaaagact
      241 tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta atcttcgccg
      301 ttacactcaa aggcggcgag gtgggaacga tatttcacag tatcggtcaa atgactatct
      361 tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctgg ttgcaaccct
      421 cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacagcg cagacacctt
      481 gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa tgcagatggg
      541 cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc cgctgttaac
      601 gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg ttaagacacc
      661 gtttcatttt tttctgatca acaacgacga aattaacgcc tttgctttct ttggcggcaa
      721 cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac tggcttcagt
      781 tatggcgcac gaaatctccc acgtcaccca acgtcacctg gcgcgagcga tggaagatca
      841 acaacgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac tggcgatggc
      901 cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc gccaggggat
      961 gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc aggtgctgca
     1021 acgctcggga ttcgatccac aggcgatgcc gaccttcctg gaaaaattgc tcgatcaggc
     1081 gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg aaagtcgtct
     1141 ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt cgtcggaaga
     1201 tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta accagctcac
     1261 cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag cggcgcaata
     1321 tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta aaacgctgca
     1381 accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta ccgatatcga
     1441 tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc gcgatttacg
     1501 caccaatccg gtgttgcagc tcaacctggc gaacgcttat ctgcaaggcg gtcaaccaca
     1561 agaagcggcc aatattctga atcgctacac ctttaataat aaagatgaca gcaacggctg
     1621 ggatttgctg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc tggctgcgcg
     1681 agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt tgagtagcgc
     1741 cagttcgcag gtgaaattag gcagcctgca acaagcgcgt tacgatgcgc gcatcgacca
     1801 gttgcgccag ctgcaggaac gctttaagtc ttataccaag atgtaattcg cattaaagga
     1861 agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa gagccgggaa
     1921 acgctgaacc tgctgaaaga aaacggcgtg gagccagaag tggtgctcta ccttgagaca
     1981 cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa tagcgcccgt
     2041 gaattgatgc gccagaaaga ggatctttat aaagaactaa acctggctga cagttcgctt
     2101 agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga gcgcccgatt
     2161 gtggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact ggaaatcgtg
     2221 ggttaagatg tgctccatcg ctagttgaag cacattgccg gatgcgacgc ttgccacgtc
     2281 ttatccggcc tacgaatact atggattaac ttcgcgtagg tcaggcgttc gcgccgcatc
     2341 cgacaataaa caccttatct acaacttcag aatttctttc acaaacggaa tggtcagctt
     2401 acgttgcgcg gtaatcgacg cacgatccaa ctgatccaac gtcataaata gcgtgcgcat
     2461 ttctctgtcg agccgcttca gcaagaaacg ccccacatct tccggcagtt caaaaccacg
     2521 caaacgcgcg cgtaactgta gcgcctgcaa cttatcttca tcagaaagtg gctgcaattt
     2581 gtagatctgc ccccagtcga gacgcgacgc gagatccggt aatcccagat tcaactgccg
     2641 cggtggacga tcgccggtga tcaacaaccg tgttttgccc gattccagaa ttcgattgta
     2701 gagatcgaaa atcgccatct cccacaactc atcgcctgca atacactcaa tgttgtcgat
     2761 acagaccagc gacaaatgct ccataccgtc gagcacttcc ggaacaaacc aggtgcgttt
     2821 atccagcggg acatagccca ccgcatcgcc acgctgcgac aattccgcgc aagccgcgtg
     2881 cagcagatgg ctgcgccccg cgccttcgcg tgcccagaga tagatgtaac cgctatgttc
     2941 ctgacgcagc acgttttgca gcgcggccag taaagaggag ttatcccccg gccagaaact
     3001 tgcaaaggtt tcgtcgtcag gaagataaag tggcaaagag agctgtgccg atgtgttcag
     3061 agatacctca accaggattt cacaaaatcg cgagaagttt accacagaat cccatgatgt
     3121 ttgaaccggg cagcaacact gcccggtcgg tacattattt gtctgttata tccgcgtctt
     3181 ctgcgtccag caccacttct tcagggcgca gcacactgat cagtttgaaa atcaaactta
     3241 agccgatacc gacgatggtc gccagcgcca taccttttaa ctccgccgcg ccgatgttta
     3301 ccttcgcgcc actgacgccg atgatcaaaa tcacggaagt caggatcagg ttctgtgctt
     3361 tgttgtaatc cactttcgat tcgatcaaaa cacgaatacc ggaagcacca atgacaccat
     3421 aaagcagcag cgaaacgccg cccatcaccg gcaatgggat catctggata gcggcagcca
     3481 gtttaccgac gcaggaaagc aggatagcga aaatcgccgc cccgccgata acccaggtac
     3541 tgtaaacacg ggtgatcgcc atcacgccaa tgttttctcc gtaagtggta tttggcgtag
     3601 agccaaagaa gccggagatc acggtcgaca ggccgttagc aaacatcgaa cggtgcagac
     3661 ctggatcgcg cagcagatct tttttgacga tattagccgt tactaccagg tgccctacgt
     3721 gttcggcaat aaccactaac gccgctggca gaatagtcag aatggcaaac cactcgaagc
     3781 gcggcgtata gagggttggc agtgcaaacc agtgagcatt aataatcggc gtggtatcga
     3841 caattcccat tgcgaaagag agcgcgtacc ccaccagcac gccaattaaa atcgggataa
     3901 ttgccaggaa accacgaaac agcacggaac ctaaaaccgt gaccgccagg gtggtaatag
     3961 agatgatgat ggttttggag tttggcgttt gcccttcagc cgggagtaaa cccgccatac
     4021 cggcagctac gcccgccagc tccagaccga tgacggcaac gattgcgccc attgccgcag
     4081 gtggaaacag cacgtccaac cagccggtcc ccgctttctt cacgataaaa gaaaccaggc
     4141 agaacagcac gccgcacata ataaagccgc ccagcgcgac ttcataccct aacggcaaca
     4201 gtaacaatac cggtgaaata aaggcaaagc tggaaccaag ataagccgga attttccctt
     4261 tacagatgaa gagatacagc agcgttccaa taccgttaaa taacagtaca gtcgccgggt
     4321 taatatgaaa taagacgggc accaggacgg ttgcaccaaa catggcgaac aaatgttgca
     4381 aactaagcgg gattgtctgt aaaagtggcg gtctttcact caccccgata gcacggcgcg
     4441 tcatagtatt atcctctgta ttatgtgtta taggcgcttt actcaaaaaa aagccgactc
     4501 ttaaagtcgg ctttaattat ttttattctt tatttcgtac caaagatttt gtcaccggca
     4561 tcgccgaggc ccggaataat gtatccgtgc tcgttcagtc cctggtcaat cgatgcagta
     4621 tacagttcga cgtccgggtg cgctttttcc agcgcagcga taccttctgg cgcagcgacc
     4681 agcaccagaa ctttgatgct gctgcagccc gcttttttca gcaggtcgat ggtcgcgata
     4741 acggaaccac cggttgccag cattgggtca acgatcagcg ccatacgctc atcgatgtta
     4801 gaaaccagtt tctggaagta cggtaccggc tccagcgttt cttcattacg gtacataccg
     4861 acaacgctga tgcgcgcgct cggaacgttt tccagcacac cgtccatcat accaagaccc
     4921 gcacgcagaa ttggcacaac ggtaattttc ttacctttga tctggtcgat ttctaccggg
     4981 ccgttccagc cttcgatagt tactttttcc gtttcgaggt cggcggtcgc ttcgtaagtc
     5041 agcaggctac ccacttcgga agcgagttcg cgaaagcgct tggtgctgat atcttgctca
     5101 cgcatcagtc ccagcttgtg tttgacgagt gggtgtttga cttccacgat cttcatactc
     5161 tttctccttt gaggggcagc cacaaaaaaa tcgacggatt atacctcctt tcttcaaggc
     5221 ggcaatatta ttttcgttga ctttagtcaa aatgataaca gtttgagata aagttatttt
     5281 atattcaaat ggttatgaaa gaagattatt ccatccgaaa actaaccttt gccctggcac
     5341 aagtcttctt tcgccgcgcg cccggggaaa agacgtgcaa aaaggttgtg taaaacagtc
     5401 tcgcaaacgt ttgctttccc tgttagaatt gcgccgaatt ttatttttct accgcaagta
     5461 acgcgtgggg acccaagcag tgaccgataa aacctctctt agctacaaag atgccggtgt
     5521 tgatattgac gcgggtaatg ctctggttgg aagaatcaaa ggcgtagtga agaaaacgcg
     5581 tcgtccggaa gtgatgggcg gtctgggcgg cttcggtgca ctgtgtgcat tgccgcaaaa
     5641 atatcgtgaa cccgtgctgg tttccggcac tgacggcgta ggtaccaagc tgcgtctggc
     5701 aatggactta aaatgtcacg acaccattgg tattgatctg gtcgccatgt gcgttaatga
     5761 cctggtggtg caaggtgcgg aaccgctgtt tttcctcgac tattacgcaa ccggaaaact
     5821 ggatgttgat accgcttcag cggtgatcag cggcatcgcg gaaggttgtc tgcaatcggg
     5881 ctgttctctg gtgggtggcg aaacggcaga aatgccgggg atgtatcacg gtgaagatta
     5941 cgatgtcgcg ggtttctgcg tgggcgtggt agaaaaatca gaaatcatcg acggctctaa
     6001 agtcagcgac ggcgatgtgc tgattgcact cggttccagc ggtccgcact cgaacggcta
     6061 ttcgctggtg cgcaaaattc ttgaagtcag cggttgtgat ccgcaaacca ccgaacttga
     6121 tggtaagcca ttagccgatc atctgctggc accgacccgc atttacgtga agtcagtgct
     6181 ggagttgatt gaaaaggtcg atgtgcatgc cattgcgcac ctgaccggcg gcggcttctg
     6241 ggaaaacatt ccgcgcgtat tgccagataa tacccaggca gtgattgatg aatcttcctg
     6301 gcagtggccg gaagtgttca actggctgca aacggcaggt aacgttgagc gccatgaaat
     6361 gtatcgcacc ttcaactgcg gcgtcgggat gattatcgcc ctgcctgctc cggaagtgga
     6421 caaagccctc gccctgctaa atgccaacgg tgaaaacgcg tggaaaatcg gtatcatcaa
     6481 agcctctgat tccgaacaac gcgtggttat cgaataatga atattgtggt gcttatttcc
     6541 ggcaacggaa gtaatttaca ggcaattatt gacgcctgta aaaccaacaa aattaaaggc
     6601 accgtacggg cagttttcag caataaggcc gacgcgttcg gccttgaacg cgcccgccag
     6661 gcgggtattg caacgcatac gctcatcgcc agcgcgtttg acagtcgtga agcctatgac
     6721 cgggagttga ttcatgaaat cgacatgtac gcacccgatg tggtcgtgct ggctggtttt
     6781 atgcgcattc tcagcccggc gtttgtctcc cactatgccg ggcgtttgct gaacattcac
     6841 ccttctctgc tgccgaaata tcccggatta cacacccatc gtcaggcgct ggaaaatggc
     6901 gatgaagagc acggtacatc ggtgcatttc gtcaccgatg aactggacgg tggcccggtt
     6961 attttacagg cgaaagtccc ggtatttgct ggtgatacgg aagatgacat caccgcccgc
     7021 gtgcaaaccc aggaacacgc tatttatcca ctggtgatta gctggtttgc cgatggtcgt
     7081 ctgaaaatgc acgaaaacgc cgcgtggctg gatggtcaac gtctgccgcc gcagggctac
     7141 gctgccgacg agtaatgccc ccgtagttaa agcgccagct ctgccgctgg cgtttttcaa
     7201 ttcacctgta aatcgcaagc tccagcagtt tttttccccc ttttctggca tagttggaca
     7261 tctgccaata ttgctcgcca taatatccag gcagtgtccc gtgaataaaa cggagtaaaa
     7321 gtggtaatgg gtcaggaaaa gctatacatc gaaaaagagc tcagttggtt atcgttcaat
     7381 gaacgcgtgc ttcaggaagc ggcggacaaa tctaacccgc tgattgaaag gatgcgtttc
     7441 ctggggatct attccaataa ccttgatgag ttctataaag tccgcttcgc tgaactgaag
     7501 cgacgcatca ttattagcga agaacaaggc tccaacgctc attcccgcca tttactgggc
     7561 aaaattcagt cccgggtgct gaaagccgat caggaattcg acggcctcta caacgagcta
     7621 ttgctggaga tggcgcgcaa ccagatcttc ctgattaatg aacgccagct ctccgtcaat
     7681 caacaaaact ggctgcgtca ttattttaag cagtatctgc gtcagcacat tacgccgatt
     7741 ttaatcaatc ctgacactga cttagtgcag ttcctgaaag atgattacac ctatctggcg
     7801 gtggaaatta tccgtagcga tagcatccgt tacgcgctgc tggagatccc atcagataaa
     7861 gtgccgcgct ttgtgaattt accgccagaa gcgccgcgtc gacgcaagcc gatgattctt
     7921 ctggataaca ttctgcgtta ctgccttgat gatattttca aaggcttctt tgattatgac
     7981 gcgctgaatg cctattcaat gaagatgacc cgcgatgccg aatacgattt agtgcatgag
     8041 atggaagcca gcctgatgga gttgatgtct tccagtctca agcagcgttt aactgctgag
     8101 ccggtgcgtt ttgtttatca gcgcgatatg cccaatgcgc tggttgaagt gttacgcgaa
     8161 aaactgacta tttcccgcta cgactccatc gtccccggcg gtcgttatca taattttaaa
     8221 gactttatta atttccccaa tgtcggcaaa gccaatctgg tgaacaaacc actgccgcgt
     8281 ttacgccata tttggtttga taaagcccag ttccgcaatg gttttgatgc cattcgcgaa
     8341 cgcgatgtgt tgctctatta tccttatcac acctttgagc atgtgctgga actgctgcgt
     8401 caggcttcgt tcgacccgag cgtactggcg attaaaatta acatttaccg cgtggcgaaa
     8461 gattcacgca tcatcgactc gatgatccac gccgcacata acggtaagaa agtcactgtg
     8521 gtggttgagt tacaggcgcg tttcgacgaa gaagccaaca ttcactgggc gaagcgcctg
     8581 accgaagcag gcgtgcacgt tatcttctct gcgccggggc tgaaaattca cgccaaactg
     8641 ttcctgattt cacgtaaaga aaacggtgaa gtggtccgtt acgcacacat cgggaccggg
     8701 aactttaacg aaaaaaccgc gcgtctttat actgactatt cgttgctgac cgcagatgcg
     8761 cgtatcacca acgaagtacg gcgggtattt aactttattg aaaacccata ccgcccggtg
     8821 acatttgatt atttaatggt gtcaccgcaa aactctcgcc gtctgttata tgaaatggta
     8881 gaccgcgaaa tcgccaacgc gcagcaaggg ctgcccagtg gtatcaccct gaagctaaat
     8941 aaccttgtcg ataaaggcct ggttgatcgt ctgtatgcgg cctccagctc cggcgtaccg
     9001 gttaatctgc tggttcgcgg aatgtgttcg ctgatcccca atctggaagg cattagcgac
     9061 aacattcgtg ccatcagtat tgttgaccgt taccttgaac atgaccgggt ttatattttt
     9121 gaaaatggcg gcgataaaaa ggtctacctt tcttccgccg actggatgac gcgcaatatt
     9181 gattatcgta ttgaagtggc gacaccgctg ctcgatccgc gcctgaagca gcgggtgctg
     9241 gacatcatcg acatattgtt cagcgatacg gtcaaagcac gttatatcga taaagaactc
     9301 agtaatcgct acgttccccg cggcaatcgc cgcaaagtac gggcgcagtt ggcgatttac
     9361 gactacatca aatcactcga acaacctgaa taaccctatg ccaatacacg ataaatcccc
     9421 tcgtccgcag gagtttgctg cggtcgatct tggctcaaac agttttcaca tggtcatagc
     9481 ccgtgtggta gatggtgcca tgcagattat tggccgcctg aaacagcggg tgcatctggc
     9541 ggacggcctg gggccagata atatgttgag tgaagatgca atgacgcgcg gtttgaactg
     9601 tctgtcgctg tttgccgaac ggctacaagg gttttctcct gccagcgtct gtatagtcgg
     9661 cacccatacg ctgcgtcagg cgctgaacgc cactgacttt ctgaaacgcg cggaaaaggt
     9721 cattccctac ccgattgaaa ttatttccgg taatgaagaa gcccgtctga tttttatggg
     9781 cgtggaacat acccaaccgg aaaaaggtcg caaactggtt attgatattg gcggcggatc
     9841 tacggaactg gtgattggtg aaaatttcga acctattctc gttgaaagcc gccggatggg
     9901 ttgtgtcagc tttgcccagc tttattttcc tggcggggtc atcaataaag agaattttca
     9961 gcgcgctcgc atggcggcag cacaaaaact ggaaacttta acctggcaat tccgtattca
    10021 gggctggaac gttgcaatgg gcgcttccgg taccataaaa gccgcccatg aagtgttaat
    10081 ggaaatgggc gagaaagacg ggataattac cccggaacgt ctggaaaaac tggtaaaaga
    10141 agttttacgt caccgtaatt tcgcatcgct gagtttaccg ggtctttccg aagagcggaa
    10201 aacagtcttc gttcctggac tggcgatttt atgtggtgtg tttgatgctt tagccatccg
    10261 tgaactgcgc ctttctgacg gggcgttacg cgaaggcgta ctgtatgaaa tggaaggacg
    10321 tttccgtcat caggatgtgc gtagtcgcac cgccagcagc ctcgccaacc agtatcacat
    10381 agacagcgaa caggcccgac gagtgctgga taccactatg caaatgtacg aacagtggcg
    10441 ggaacagcaa ccgaagctgg cgcatccgca actggaggcg ctactgcgat gggccgccat
    10501 gctgcatgag gtcgggttga atatcaacca cagcggtttg catcgccact ccgcttatat
    10561 tctgcaaaac agtgacttgc cgggttttaa tcaggaacag cagctgatga tggcgacact
    10621 ggtgcgctat caccgtaaag cgattaagct cgacgatctg ccgcgcttta ccttgtttaa
    10681 gaagaaacag ttcctgccac tgatacagct attgcgcctt ggcgtattac taaacaatca
    10741 acgtcaggca accaccacac cgccaacatt gacactgatt actgatgaca gtcactggac
    10801 actgcgtttc ccgcatgact ggtttagtca gaatgcgctg gtactgcttg atctggaaaa
    10861 ggagcaagaa tactgggaag gcgtggctgg ctggcggttg aaaattgaag aagaaagtac
    10921 cccagaaatc gccgcttaat gctttgcggg ccgacgagaa atgtcggccc gcattattca
    10981 ggcactttcg cgaatgggtt cgatttcatt cagcgtatca attaacggtt gcggcttacc
    11041 aataagataa ccctgcatat aatcgatccc caaagagagc accgcctcgc ggatctcttc
    11101 gttttcaacg tactctgcca ctacccgcat tttcttcatt cgcgccagat ggcaaatcga
    11161 cgccacaatc tgataatcca gactatttga cacaatattg cggataaagc tgccgtcaat
    11221 tttaagcaga tcggcattca catttttaag ccgtgcatag ctggcgtagc cggtgccgaa
    11281 atcatcaatc gcaatctggc agcctaattc ctgaagatgc tgcaaggtga tttgcgcctg
    11341 cttaacattg gtcagagcat tactttcggt gacttcaaaa ataagttgcc acgcttcaat
    11401 ctgatattta gccagcaact gactgacttc aacaggaaaa cgagcctgac ataccgaggt
    11461 tggagacaga ttaatagcaa aacggtgagc gggcatcttc gctctgtttt cagccataaa
    11521 ttgcagcgta tgctcaatga cccacatgtc gatactcgac gataaaccaa attcgtgcgc
    11581 gaccggcaag aagctatcgg ggccgatcag ttcatcattc tcacctttca tgcgcagaag
    11641 aatttcatgg taaacatcac cacgcatacc ggtaatcggc tgggccatca ggaaaaaatg
    11701 gttgtgttcc agcgcctgct gtagtcgatt catcatcgcg actttatctt tcaattcgcg
    11761 ttgcaaatac attgccccgc aacgctgcat attttccggg gcgttggtca cgatggaaag
    11821 ttcggcgacc gtatttagct ctcccagcag caggtagata tgattcactg gcgagcgcac
    11881 atagcagtaa ctgacgccaa tctgcggttg catcggcatc ccatcccaaa agaaacgaaa
    11941 ttgcttgaga tgactatcca gtgcggtaat gcgctcctgg tgcgattctg tattcagtcg
    12001 cagcgcgaga tcgttacccg aaagctgata aacatcttca cccggttcca gcaagggtga
    12061 cagccagtga gaaagttttt gcttgtattg aatccgcagc atgatgccat agttcttaac
    12121 cagcatttcc atgccaggga tgcgtaaata acaaagcgca gaccaagggg catcacgcaa
    12181 cgcgcgattc agggcgcgaa catttggcag atgaaccacc gggtccacat acgcaagccg
    12241 ctgaatgcgt ctgactaccg ctcgctgacg agttgccagt acagccatat aattgacaat
    12301 caaagagaat accagataac tggaggaggt tatggtcagc tgcgtggtat agccaggata
    12361 aatgggaatg taattttgat agctgtggat gctgatcatc aacacgaccg cccagagcag
    12421 cgaaatcagc ttataaccat agcgcattgc tccccacatc atcaggggca gcaataatga
    12481 caaggtataa ttggtgctaa aaattgtgct tttttcattt aacggcatgc ataacagcaa
    12541 caataaagca cctaatgcca gtagccagag cgcgaactct tttttggtga ctttggcatc
    12601 aacctgctgt tttaattgcg aataatagct acgtaaataa aatggatttc gcactacccg
    12661 aatgatgaag tagcacagcg ggacaccgat cagattaccc accagtaagg cctgataatt
    12721 gattaaagtc ccgaggttaa aaggcatgac accgaccaga ttttctctgc tcgccagtaa
    12781 tcctacaaat gcagcaaact ggaaaagtat cagaaacagc gttgcaggaa acactatctg
    12841 ccagaatata cgctgggaaa ttaaacgggt atcaccatgt gaaacgttgt tacgccgggg
    12901 agtaaagacc ctgtaaccgc cccaacagag aatgataatg ataataaaat tagccgttaa
    12961 tgagagcgtt tcgtaaaaac ccagctctgc atatttgcgg acaaaaatcc ctaaggtgat
    13021 ccctggcaat gccgcccagc tgaaaaacat catcatgctg atcatcagtg ccaaaggcag
    13081 ataaaagaga aaaacctcac cggaagaaat atgcgcgaaa gtattaatgt gggcgaaaat
    13141 gggcaagatt aaagaaggca ggaacagcgg aagcccccac catttatcac gtatttttat
    13201 ataagttgca ttcagtttca tagatgctca gcagaatccc ccacatcctg aaggaggtgt
    13261 attcagacag gcatcccacc tgacttcgaa tgatgattat tcatcactat agagagcatt
    13321 gattctaagt gtcatatgaa agttccaatt gatatatatc aaacaaaata accctgatta
    13381 atgaattatt acgtttatca tgttaattca tcattattac atcatcattg taaataatta
    13441 aattaacttc cataacatta aaatatgtat ccactgacgc ttttttacat aacgaagaat
    13501 tgaccatttt gtcctgttgt gccttaatgt aagtaccgtc cacagcgtgg gacatacttc
    13561 aaggaacctt ttgtgagtca ggcaactagt atgcgaaaac gacaccgatt taacagtcgc
    13621 atgacccgta tcgtactgct catcagcttt atcttcttct ttggccgttt tatctactcg
    13681 tccgtcggtg cctggcagca ccatcagagc aaaaaagaag ctcagcaatc cacactctcc
    13741 gtcgaatcac cggtacaacg ttagcggtta ccttctccac tttcacagaa cataacggca
    13801 cttcgctgtc ggatgctttt gctctttggg attatcaaag cggcagatat tctttcatct
    13861 taaattttac gtctttgtcc tgactgatgt ttatcctgtt tggctgcgaa ataaatataa
    13921 aattaatata tatgttgtaa tgatatattt ttataaatta ttccctgcgt gaattttaat
    13981 aaatttaatc tatcccttta tacgcaatac atttactttc ctcttttgat gatcttaaat
    14041 gtcttatttt tcgtaatgtg tataacaagg aatagtgatg aaatttaaaa aatgtcttct
    14101 gcctgtggca atgttagcgt cattcactct ggcaggatgc cagtcaaatg ctgacgatca
    14161 tgccgccgat gtttatcaaa ccgatcaact gaataccaaa caagaaacta aaaccgttaa
    14221 tattatttcc attcttcccg caaaagttgc cgtagacaac tcccaaaata aacggaacgc
    14281 acaagccttc ggcgcgctta ttggcgcagt cgctggcggt gttatcggcc acaacgtcgg
    14341 ttctggcagc aattccggaa cgacggcagg ggcagttggc ggcggagctg taggcgcggc
    14401 agcgggttct atggtgaatg ataaaacctt agtggaaggt gtttctttaa catataagga
    14461 aggcaccaaa gtgtatacct ccacccaggt gggtaaagag tgccagttta cgacaggttt
    14521 agccgttgtt attaccacaa cgtataacga aacgcgtatt cagccaaata ccaaatgtcc
    14581 tgaaaagagc taataatcag gaggagtcat gaagaaagtt tttctttgcg ccatcttagc
    14641 ctccttaagc tatccggcta tagcctcatc attgcaggat caactctctg ctgtcgcaga
    14701 agcggaacag caaggtaaaa atgaagagca aaggcagcat gacgaatggg tcgcggagcg
    14761 caacagggaa atccagcaag agaagcaacg tcgcgcaaat gcccaggccg ccgcgaacaa
    14821 aagagcggca acggcagcgg caaataagaa agctcgtcag gataaactgg acgccgaagc
    14881 cactgcggac aaaaaacgcg atcaaagtta tgaagatgag ctacgcagct tagagattca
    14941 gaaacaaaaa ctggcgctgg cgaaagaaga agcccgcgtt aagcgcgaaa acgaatttat
    15001 cgatcaggaa ctgaagcaca aagctgcgca aaccgatgtg gtgcaatctg aagctgacgc
    15061 aaacagaaat atgactgaag gcggtcgcga tctgatgaaa agcgtgggca aagcagaaga
    15121 gaacaaatcg gacagctggt ttaattaatc gatgttagta acttcaagcc tatgattctt
    15181 gaagataaaa aaccctctgt agtaacggag ggttttgttc attcatagtg cagggatcaa
    15241 aatcattccc actcaatggt agctggcggc ttgccgctga tgtcatacac cacgcgggaa
    15301 ataccgttca cttcattgat aatgcggttg gaaacgcgac cgaggaaatc gtacggcaga
    15361 tgcgcccagt gtgcggtcat aaagtcgatg gtttcgacag cacgcagaga gacaacccag
    15421 tcatacttac gaccatcgcc cattacgcca acggaacgta ctggcaggaa gacggtgaac
    15481 gcctggctga ctttgtcgta caggtccgct ttacgcagtt cttcaatgaa gatggcgtca
    15541 gcacggcgca gcaggtcaca gtactctttc ttcacttcac ccagaacacg aacgccaagg
    15601 cctggtcccg ggaacggatg acggtacagc atgtcgtacg gcaggcccag ctccagacca
    15661 atcttacgca cttcgtcttt gaacagctct ttcagcggtt caaccaggcc catcttcatc
    15721 tctttcggca ggccgcccac gttgtggtga gatttgatga cgtgtgcttt accggttgca
    15781 gaagccgcag attcgataac gtcagggtag atggtgccct gcgccagcca cttcacgtct
    15841 tccagtttca gcgcttcttc atcgaatact tcaacgaaaa cgcgcccgat gattttacgt
    15901 tttgcttccg gatcgttttc gccagccagc gctgacagga agcgatcttc tgccggtacg
    15961 tgaacaatgt taagaccaaa gtgatcgcca aacatatcca gaacctgctc tgcttcgttg
    16021 agacgcagca ggccgttgtc gacgaatacg caagtcaggt ttttaccgat agcacggtgc
    16081 agcagcattg cggttacgga ggaatccaca ccaccagaga ggccgaggat gactttatcg
    16141 tcgcctacct gctcgcggat gcgagctaca gcatcgtcga taattttcgc tggcgtccac
    16201 agggcttcac actggcagat atcacgcaca aaacgctcca gcatgcgcat accctggcgg
    16261 gtgtgagtca cttccgggtg gaactgtacg ccatagaagc gtttttcttc gttagccata
    16321 atggcaaacg ggcagctttc ggtgctggct acggtgacga agtcggacgg gatagcggta
    16381 actttatcgc cgtggctcat ccagacatcg agcagcggtt taccgtctgc ggtcagcgca
    16441 tcttcgatac cgcgaaccag tgcgctgtcg tttacgactt caacctgcgc gtagccaaat
    16501 tcacgttcgt tagaggcttc aacgtgaccg cccaactgca ttgccatggt ctgcatgcca
    16561 tagcaaacgc cgaatatcgg tacgcctgct tcaaagacat actgcggcgc acgcggactg
    16621 ttttcttcag tagtactttc cgggccgccg gaaagaataa tgccgcttgg attgaagtca
    16681 cgaatttgtg cttctgtcac atcccacgcc cacagttcgc agtaaacacc cagctcacgc
    16741 acgcggcgcg caaccagttg agtgtactga gaaccgaagt ccagaatgag gatgcgatgc
    16801 ttatgaatgt tttccgtcat tgacgcttat tccgaggcaa gtgaaacaga taatataaat
    16861 cgcccgacat aaagtcgggc gaagagaatc aggagcccag acggtagttc ggggactctt
    16921 tagtaatggt cacgtcgtga acgtggcttt cctgaatacc cgcaccgctg atacgtacaa
    16981 actccgcttt agtacgcagt tcgtcgatag taccacagcc ggtcagaccc atacaggagc
    17041 gcaggccgcc catctgctgg tgaatgatct ctttcaggcg acctttataa gctacgcgac
    17101 cttcgatacc ttccggcacc agtttgtcgg cagcgttatc gctctggaaa taacggtcag
    17161 aggaaccttt ggacatcgcg cccagggaac ccataccacg gtaagattta taagaacggc
    17221 cctggtagag ttcgatttca cccggagatt cttcagtacc cgccagcatg gaacctacca
    17281 tcaccgcgct tgcgccagcg gcgatagctt tggcgatgtc gccggagaag cgaataccac
    17341 catctgcgat aaccggaata ccggtgcctt ccagggcttc aactgcgtca gcaacggcag
    17401 tgatctgcgg aacacctacg ccagtaacga tacgagtcgt acagatagaa ccagggccga
    17461 taccgacttt aaccgcgctg caaccagctt ctgccaaagc gcgtgcacct gcagctgttg
    17521 ccacgttgcc gccgatgatt tgcagatccg gatatttagc acgggtttca cggatacgct
    17581 gcaaaacgcc ttctgagtga ccgtgggagg agtcgatcag cagaacgtca acgccagcgg
    17641 caaccagcgc gtcaacacgc tcttcgttac ccgcacctgc gccaactgca gcaccaacac
    17701 gcagacggcc ttgctcgtct ttacaggcgt tcggtttacg ttccgctttc tggaagtctt
    17761 tcacggtgat catgccgatc aggtggaatt cgtcatcaac caccagcgct ttttcaacgc
    17821 gtttttcgtg catttttgcc agcaccactt cacgggcttc accttcacgc acggtgacca
    17881 gacgctcttt cggcgtcatg taaacgctaa ccggctggtt caggtcggta acaaaacgca
    17941 cgtcacgacc ggtgataata cccaccagtt cgttttcttc ggtaacgacc ggatagcccg
    18001 caaaaccgtt acgctcggtc agttctttca cttcgcgcag cgtcgtggtt ggcagaacag
    18061 tctgcggatc agtcaccaca ccagattcgt gttttttcac acggcgaact tcttctgcct
    18121 ggcgttcaat ggacatgttt ttgtggataa agccgatacc gccttcctga gccagagcaa
    18181 tagccaggcg cgcttccgtt acggtatcca ttgctgcgga aagcataggg atattcagac
    18241 gaatagtttt cgtcagctgg gtgctgaggt cagcagtatt cggcagaacg gtagaatgag
    18301 caggaacgag gagaacgtcg tcaaacgtca gagcttcttt agcgatacgt agcatgggca
    18361 atatctcgac cagagtggtt aataaatatt gccgcggcat tatacagagc gtaaccgatt
    18421 gcatctaccc ctttttgcaa aaaatgcttg ctatccccga agggcgggtt actatcgact
    18481 gaataacctg ctgatttaga atttgatctc gctcacatgt taccttctca atcccctgca
    18541 atttttaccg ttagtcgcct gaatcaaacg gttcgtctgc tgcttgagca tgagatggga
    18601 caggtttgga tcagcggcga aatctctaat ttcacacaac cggcttccgg tcactggtac
    18661 tttacactca aagacgacac cgcccaggta cgctgcgcga tgttccgcaa cagcaaccgc
    18721 cgggtcacct tccgtccgca gcatgggcaa caagttttag ttcgcgccaa tattacgctc
    18781 tacgagccgc gcggcgacta ccagataatc gttgagagta tgcagccggc cggtgaaggg
    18841 ctgctgcaac agaagtacga acagctcaaa gcgaagttgc aggctgaagg tttgttcgat
    18901 cagcaataca aaaaaccact tccctcccct gcgcattgcg ttggtgtgat cacctcaaaa
    18961 accggtgctg cgctacatga tattttgcat gtgttaaaac gtcgcgatcc ttctctaccg
    19021 gtgatcatct accccacctc cgttcagggc gatgacgcgc cggggcaaat tgttcgcgcc
    19081 attgaactgg cgaatcagcg caatgagtgc gacgtgttga tcgttgggcg cggcggcggt
    19141 tcgctggaag atttatggag ttttaacgac gaacgcgtag cgcgggcgat ttttgccagc
    19201 cgcattccgg tcgtcagcgc cgtcgggcat gagacggatg tgaccattgc cgattttgtt
    19261 gccgatctgc gtgcgccaac gccgtctgcc gccgctgaag tagtgagccg taatcagcaa
    19321 gagttactgc gccaggtgca atcggcccgt caacggctgg agatggcgat ggattattat
    19381 ctcgccaacc gtacgcgtcg ctttacgcag attcatcatc gcttgcagca gcagcatccg
    19441 cagctccggc tggcacgcca gcaaaccatg cttgaacgcc tgcaaaaacg gatgagcttt
    19501 gcgctggaaa atcagcttaa gcgtgccggg caacagcagc agcgattaac acagcggctg
    19561 aatcagcaaa atccacagcc gaagattcat cgcacgcaaa cgcgcattca gcaactggaa
    19621 tatcgtttag cagaaatcct gcgcgcacag cttagcgcca cgcgtgaacg tttcggtaat
    19681 gcagtaacgc acctcgaagc cgtaagccca ctgtcaacgc tggcgcgtgg atacagcgtt
    19741 actactgcta ctgacggcaa tgtactgaaa aaagtgaagc aagttaaagc gggtgaaatg
    19801 ctaaccacac gtctggaaga cggctggata gaaagtgaag taaaaaacat ccagccggta
    19861 aaaaaatcgc gtaaaaaggt tcattaagcc agcacgaatt caacgcgttt tttcgatatc
    19921 aagccatgcc cgtgctgaca gaagtaatct actgcaccgc aggcttttaa cacctgcaac
    19981 ggttgatggc agtctggaca aagagctttc atttcaataa attccccgca ggatgggcaa
    20041 cgcgcatggc cgttatcctg atcaagcaca tgctgacatt ttggacaatg cagttccata
    20101 tgaattcctc cggtagccat tccctctaca ttcatagagg gaatggcaga taaaatactt
    20161 acggataatt atttattttt cttgatgtgc ttcatcagac gcttacgttt acgcatctgg
    20221 gttggcgtca gggtgttacg cttgttcgca tacgggtttt ccccttcttt gaactgaata
    20281 cgaatcggcg atcccattac gtccagcgat ttgcggaagt agttcatcaa gtagcgcttg
    20341 taggaatcag gcaggtcttt cacctgatta ccgtgaatca ccacaatcgg cgggttataa
    20401 ccaccagcgt gggcatattt cagcttcaca cgacgaccgc gtaccagcgg cggttggtga
    20461 tcttcaacag ccatcgtcat gatgcgcgtc agcatagagg tccccacacg acgggtggag
    20521 ctgtcatacg cttcacgtac tgattcaaac aagttaccaa caccactgcc gtgcaaggca
    20581 gagataaagt gcacacgagc aaaatcgata aagcccagac ggaagtccag cgtttctttc
    20641 acctgctctt tcacttcctg gctcaggcca tcccacttat tcaccacaat gacaagtgag
    20701 cgcccactat tgagaataaa gcccagcagc gagagatcct gatcggaaat accttcgcgc
    20761 gcatcaatca ctaacatcac cacgttggcg tcttcaatgg cctgcaacgt tttgattacg
    20821 gagaatttct ctacagcatc ggtgattttg ccgcgtttac gtacgccagc ggtgtcaatg
    20881 agcacatact cacgtccatc gcgttccatt gggatgtaga tgctgtcacg cgtcgtgcca
    20941 ggcatgtcgt aaacaacaac gcgctcttct ccaagaatac ggttagtgag tgtagactta
    21001 cctacgttcg gacgacccac aatcgccagt ttgatcggca gactttgcgg gtcgaagtcg
    21061 tcttcctctt cttcttcgcc gttctcttcc gcttcaaatt gcgcccagta ttcagcgtct
    21121 tcgtcgactt cctcttgcgg tgcgagatct tccatccacg gcagcagcac atgctccagc
    21181 agacttaata cgccacgacc gtgagacgcg gcgatcgggt agatttcacc taaaccaagc
    21241 gagtagaaat caaccactgc ctgatcggga tccagaccgt cagttttgtt tgccaccaag
    21301 aaggtcggtt tttcacggga gcgcagatgt ttggcaatcg cttcatctgc cggcatcagg
    21361 cccgcgcgcg catccaccat aaacagtacg acgtccgctt cttcaatcgc cagcagcgac
    21421 tgttccgcca tgcgggtttc tacaccgtct tctgtgccat caatcccgcc ggtatcaata
    21481 cagataaact cacggccttc aatttccgca cgaccgtact tacggtcacg agtcagaccc
    21541 gggaaatccg caaccagcgc atctcgggtg cgagttagac ggttaaataa cgtggatttt
    21601 cctacgttag ggcgcccgac aagcgcgacc acaggtacca tgtttaaagc ctcattttta
    21661 taaatcatca gacaacgcac gctatattcg cgtcgttgtt aaaaacagga aaacggcccc
    21721 tgtccaggag ccgttttcaa agtgaacgac agagacgatt aacgtgtaat agagtacacg
    21781 gttccgtctt ttgcctggat cagcagtttg ccgtcagcgg caaccggttc agtctggaaa
    21841 ccggaactat caactttttg ctgggcaacg aaacgaccat cttcgacgtt aatccagtgc
    21901 agataacctt cactgtcacc gaccaccagg ttgccattat acagcaccgg agaagtcagc
    21961 aggcgatgca gcagatcgct ttgtgcccac agcgtaacgc cgccatcaat ggtcaacgcc
    22021 atcacccggt cattttgatc gaccagatag atgcgattgc catcgacgat gaaatcattc
    22081 accgaaccca gttcgcgttt ccacataatc tgaccactgc gcagatcaag cgccgtcagg
    22141 ttaccattat aggccagcgc gaaaacaacg ccgttaacaa cgacgggagt cgtgtcaaca
    22201 tcgctcagac ggtcaatttc ggtagaaccg gtcgcctggg aaatacgctg ctgccaaatc
    22261 atctggccct gttccatcag cactgcgctg acgcgaccat tatcgccacc cacgacggcc
    22321 gcaccaaaag ccgttgccgg cgcagactcg ccacgcaaag agagcgaagg catatcgagg
    22381 ttaactgtcc atttgacagc gccgtcagct tcgttcagcg cttgtaactg accgttactg
    22441 gtgtggatta acaccagacc gtcgctgacc accgggcgcg aaagtgcttc acccgcgact
    22501 ttagtttgcc atgccacagt accatcgctg gtattcagcg cgtaaacctg cgccttttcg
    22561 ctgccaatgt agacatgccc accagacacg gtcacaccgc cagaaagtaa tgcaggctct
    22621 ttagagaacc agccatcttt ctcggccagg ctgacagacc agatttcttt gccatcatcc
    22681 gcattcagcg cttttaccaa accagcgcgg tccgctgcat agacaacgtt gtccgccagt
    22741 gccggatgaa gattggaata gaagttgcca atgccgctac caacggaagt gctccacgcc
    22801 gtggtcggcg taaactggtt ttcaacggtt ggcaatgggg acatctttac cacatcttct
    22861 tcgctgttaa acagcgaaca gccgcttaaa agggtaacgg aaagcagtcc tggcagcagt
    22921 aatttacgca attgcatcgg gtccctctca gatggacaaa ttattaattt tcatctgcat
    22981 catttcgctc agtgccggag taacatcgct tttcacgcct gcttcccatg cactacgcgc
    23041 accttgctta tcacctttgc tcagcaatgc ttcaccacgc aggtcggcaa caatggcagc
    23101 ccacccttca cctttgatgg tatcaagggt tttcagcgcg gcatcagcct gcttgagctg
    23161 tacctgaacg cgagcaagac gcagatttat cacggctttg agattttcat cgctcgtgtc
    23221 tgccagcccc tgttgtaact gggcggcagc tttctctagt tcatttttgt caacaaattg
    23281 ctgcgcaagt tccaaagaag ccagcgcacc ataagtattt ttattttcag cagcaaattt
    23341 ttccgccgcc gggatgctat ccggtttgcc ttcgctcact gcggtaaccg cattttgata
    23401 ggcaagagaa gcggagcgtg cagaatcaac ctgatggctg ttccagtagc gccagccaat
    23461 cagtgcgcca acgcccaaaa tcaccccaac agccagtgct ttgccatttt cagcaaaaaa
    23521 gcgtttaacc gcttctacct ggtcgttttc gttctcgtaa atttccacgc tgtccttctc
    23581 cttccttaac ccagtaacgt gcgcaaatgc gcggctacgc tatcctgcgc aactgccgtt
    23641 tgctcaccag agcgcaaatc cttcactact gctgtgccgt tagccacttc agactcaccc
    23701 agcaccacag caacgcgggc accccattta tcagcacggg caaactgttt cttaaagttg
    23761 ccgccgccgt ggttggtcat caatttcacg cccagtaatt catcacgcag acgctcagct
    23821 aatgccatag ccgcagattg tgtatcagca cctgaagcca ccaggtatat atcgacaaca
    23881 ggatcggctt taaattccgg attaacggcc tgtactaaca atacaagacg ttcgaggccc
    23941 atcgcaaaac cgacagccgg tgttgcacga ccgcccagtt gttccacaag accgtcataa
    24001 cgaccgcctg cacacacggt gccctgggag ccgagactgt tagtcaccca ctcgaaaacg
    24061 gtacggttat agtaatccag accacgcacc agacgctggt ttacggtgta agcgatcccc
    24121 gcgctctcca gctgtttgca cagaccggca aaatgctcac gagattcctc gtccagataa
    24181 tcacctaatg ccggagcgtc gttgagaagc gcctgcactt ccggattttt tgaatccagc
    24241 acgcgcagcg ggttagtgta catgcggcgt ttgcagtctt cgtccagctt ttctttatgc
    24301 tgctcaagga atgccaccag cgcatcgcgg taattggcgc gtgcttccag cgaaccaata
    24361 gaattcagct caagagttac gtgttcgaag atacccagcg cacgccacca gcgggcagtg
    24421 agcataatca gttcagcgtc gatatccgga ccttgcagac cgaaaacttc gcagcccaac
    24481 tgatggaact gacgataacg ccctttctgc ggacgctcgt gacggaacat cggcccgata
    24541 taccacagac gctgttcctg attgtacaga agaccatgct cgatgccggc gcgtacacag
    24601 cccgccgtcc cttcagggcg cagagtcagg ctgtcgccat tgcgatcctc aaaggtgtac
    24661 atctcttttt caaccacgtc ggtgacttca ccaatcgcac gtttgaatag cggggtctgc
    24721 tctacaatcg gcaagcggat ttcactgtaa ccgtagctgc cgagcacgtt tttcagtgtg
    24781 ccttcaatgc gctgccagat ggccgtttcg ccaggcaggt aatcgttcat gccgcgaatg
    24841 gcttgaatgt tttttgccac gtttattctc tttctgaata taaaaatgaa ccctcaacgc
    24901 ttccctcaat gtttcgggag ccatgcgggt tcaatcatac acgggaagcg aggcgcttcc
    24961 catcacgtta ttatttttca acctgctgaa cgtcaattcg acgcgcttcg tccagctgac
    25021 tggctttcgc acgaatgcgt gcttccagct ggtcgatcat atcgttgttg tccagacggt
    25081 ctttgcgcac gccatcttca tagaggccgc ttttcttgtt gccgccggtg acgccgagtg
    25141 tagaaaccag cgcctcacct gggccattca ccacgcagcc gataatcgaa acgtccatcg
    25201 gagtgatgat atcttccagg cgttgctcca gcgcgttaac cgtaccgata acatcaaatt
    25261 cctgacgcga acaggtcggg caggcgatga agttaatccc tcgcgaacgg atacgcagcg
    25321 atttcaaaat atcgaaaccg actttgatct cttcgaccgg atcggccgcc agcgatacgc
    25381 gcagcgtgtc gccgatgcct tccgacagca gcagacctaa accaatggcg gattttactg
    25441 ccccgctgcg tgcaccaccg gcttcggtga tccccagatg cagcggctga tcgatctgtt
    25501 ttgccagcaa acgataagac tcaacagcga ggaagacgtc agacgctttc acgctgactt
    25561 tgaactgatc gaagttcagg cgatcgagat gatcaacatg acgcatggcg gattccagca
    25621 acgcctgcgg cgtcggttcg ccatactttt cttgcagatc tttttccagc gatccggcgt
    25681 taacgccaat acggatcgga atgtttttat cgcgcgcaca gtcaaccacc atgcgaatac
    25741 gctcttcatt accgatatta ccagggttaa tacgcagaca atcgacgccg tattccgcta
    25801 ctttcagcgc aatgcgatag tcgaagtgga tgtcagccac cagcggcacg ttaacccgct
    25861 gtttgatgag tttgaacgct tctgccgcgt ccatcgtcgg tacggagaca cggacgatat
    25921 cagcgccaac gcgttccagc gccttgattt gattgaccgt tgcttcgaca tctgtcgtac
    25981 gcgtattggt catggactgt acggcgatgg gagcaccatc gccaatcggc acattcccaa
    26041 cgtaaatacg tgttgatttt ctacgttgaa ttggagcctg gttatgcatg aaaaatctcc
    26101 cgcgttaccc gtctgttact gcgccggtga ttgttcggca ttgagggtca gacgcgcaac
    26161 ctggttagtt ctgataaaac gactcagatc gacaggtttc ccttgatact ggatctgtac
    26221 tgcggctggc gcaccaattt tcagtttata cggtgcctgg ccggttaaat tcaaattacc
    26281 gtctttacgc tgcatcccgc taaacaattt tttaccggta gcatcagtga cctccagcca
    26341 gcaatcggca gtaaagttca tcaccagcgc attcggatca gccgccggcg tggtcacgcc
    26401 agcctgatcg gttggcaacg gcgcagcacc atctggcgtt gttgctgccg ttggtaccgg
    26461 ggtcgcggcg gtatcaacat ttgcctgcga aggcgaaaca accgcattct gttgcggatc
    26521 aacagctggt gctggcgcag ttacggcagg tgtttgcgtg ttggttgcgg tagtatccac
    26581 agacgctggc ggcgtggacg ttgtagccgg gtctgtagtt gtcgacgtat ttaacggaac
    26641 actctgcccc tgctcgctat tactgctcag ttccgccgaa gattgatcgg ccatagtggt
    26701 gatctcttcc tgctgagctt tgtggtcttg ccaccaccag gcaccgctca ggccgataac
    26761 cacaaacaac accagccagg tgaaggtcat cagccagccg tcgcgttttt tgcggcgttt
    26821 accgagggaa aaactctgca ttggcgcaac ttttgcagcc cgaagtggag cctgcttttc
    26881 cagccctggc agcagttctt cttctggaat atgtaccaga cgcgcataag agcggatata
    26941 tccgcgcagg aatgttgaag caagatcggc gggtgcctta tcttcttcaa tgtcgcgtac
    27001 cgtggaaacc ttcaggcaaa gtcgctcggc aacggcctgc tgactaagtc ctagttgttc
    27061 gcgagcatta cgcaggcgag cgccggtagt aagtgcttca ttttggtcgt gcgtggcttc
    27121 agtattcatt cgctgcaggt acgtttaaat gagaattgag atgccggtga accatcatgc
    27181 tcacccacat cgcgaaacat ccgttaagtt aaccgttatc atacagtata agaccgtctg
    27241 tgtgctcgtg acaaagccca cattcactga ggctaactta ctgttgcatc gttacatact
    27301 gccttaaagt cagcaaaaac gcaccgttaa tattgaccag acaattgcaa cttaatactt
    27361 cattcataaa agattacgcc acggtacata aagtaaccgt ggcgtaatgg ctatcagacc
    27421 gctttaatat cgatggcttc accctgcatc cgtttacgca gagtacgttt cgtacggtcg
    27481 ataacatcgc ccgccaactg accacaggca gcatcgatat catcaccacg cgttttacgc
    27541 acaatagtgg tgaaaccgta gctcatcagc acttttgaga aacggtcgat acggctgttc
    27601 gaactgcgtc catacggcgc gcccgggaac gggttccacg ggatcaggtt gatcttacac
    27661 ggcgtatctt tcagcagttc cgccagttgg tgcgcgtgtt cagtgccgtc gttaacgtgg
    27721 tcaagcatca cgtattcaat agtgactcgg ccctgattgg cgttggattt ctccagataa
    27781 cggcgcaccg cagcaaggaa cgtttcgata ttgtactttt tgttgatcgg cacaatttcg
    27841 tcacgaattt cgtcgttcgg cgcgtgcagg gaaattgcca gtgcaacgtc gatcatatcg
    27901 cccagtttat ccagcgccgg aactacaccg gaagtggaaa gcgtcacgcg acgtttagac
    27961 aggccaaaac cgaaatcatc aagcatgatt tccatcgccg gaacgacgtt gttcaggttg
    28021 agcagcggct cgcccatgcc catcatcacg acgttagtga ttgggcgctg accggtgact
    28081 tttgctgcgc cgacgatttt cgccgcacgc cacacctggc caataatttc cgatacccgc
    28141 aggttgcggt taaagccctg ctgggcggtg gaacagaatt tacactccag cgcacacccc
    28201 acctgcgaag agacgcagag cgtggcacgg tcgtcttccg ggatatacac cgtttcgacg
    28261 cgctgatcgc caacggcgat cgcccattta atggtgccgt cggatgaacg ctgttcttca
    28321 accacttccg gtgcgcggat ttccgccacc tctttcagtt tgccgcgcaa cactttgttg
    28381 atgtcggtca tctcatcaaa gttgtcgcag caatagtgat acatccactt catcacctga
    28441 tcggcgcgga aggttttttc acctaaatct ttaaaaaact cccgcatctg ctgacggttg
    28501 agatccagca ggttgatttt tccatctttc gtggtgacgt tttcaggtgt gactaattgt
    28561 tcagacatat gctattccgg cctcgttatt acacgttatg gcccctggag ggttgaaaaa
    28621 agaaacgccc cggtgagcgg ctgctcgtcc gggggcgctg cattgtacaa attctggcgc
    28681 acggatgcca cgtttgcacg cggcatttac gaaattatta acgggtgcgc gggcacactt
    28741 cgccttcgcc gaagaaataa gcgatttcgc gagcggcaga ttcgacggaa tcagaaccgt
    28801 gggtaccgtt ttcggtcagg ctgtcagcgt aatcagcgcg cagagtacca gccagtgcgt
    28861 ttgccggatt ggtcgcgccc agcagatcgc ggtgacgctg aacggcgttt tcaccttcca
    28921 gcacggaaac cacgatcggg ccagaggtca tgaattcaac cagaccatca aagaacggtt
    28981 ttccatcgtg ttcagcataa aagccacgtg cctgttcaac ggtcaggtgc agcattttgg
    29041 tgccgacaat tttgaaccct gcagcttcaa agcgcgcaaa gatattacca atgacgtttt
    29101 ttgctaccgc gttcggtttg atgatggaaa aagtacgttc aatagccatt tttacctctg
    29161 taaattgttc tgttgttgtc tgtaccagcg tacgagatgg cgcggattat aatgagcaac
    29221 agggccgttg actattgatg aaggtaacat ttttttaaaa taaaatgagt tttagcaaca
    29281 atcagggaca aaacagacta ttgcatgaca aatttcactg tcgcgatttg ccccacatca
    29341 tccatcacca gcaattgata atcaccttta tccgtcaaat gcagggtcac gttgcgcccg
    29401 cgttcagtta acggttcgcc attcagaaac caccagcgtt cacctgcccc tccacttgat
    29461 tgcaacggca aagtcgcttc cgctgcgccc ggtaaacgtt taataatcgc gccatcgcga
    29521 acgcctgtca gctgcagagg gagctgggca tcgtgaccgt atggcgggca aattgtcgag
    29581 gccggtggta agcgcacagc gcgacgttct gatgcgggca gccagggttc cagcggtagc
    29641 ggccagacat ttaccatttc ttgcttcgcc tgcgggcagt cagcggcgac gcgctgccct
    29701 ttgtcatcca gccagatggg gaagcgaatg ccattaatcc cttcctgctc tgacagtaac
    29761 agcgttggcg gctggctgcc atccagcagc caggttgcca ggcggcggcg acagttaccg
    29821 tcaccttccg gcaaagactg tccgcccggc cagcagataa cgccacgagt gactgagttc
    29881 gggcgcgggt cttccggcag attcgcactg cgcgacagta agatattatt gacctgattc
    29941 aacaatggta cggcactggc aaagccaaac tgaccaacaa cgggcgtgcc gtcaggtctg
    30001 ccagtccaga tcccaatgac atagcgagcg ttaaccccaa tcgcccaggc gtcacgatag
    30061 ccatagctgg tgcccgtttt ccatgccagt ggggcgacgc gcggcaaggc actatccggc
    30121 aagggttgcg cttcatcagc cataatccgg cgaatgatcc acgccgcccc cgacgacatt
    30181 aaaggccgtt caagcagcgg atcgtcaggc tgtaagcgca atttgcctgc cttgccgtgg
    30241 cgagcaaacg cggtatacgc tgccgccata tcttccagtt ttgcaccagc gccgccgaga
    30301 atgagtgaaa gattcggcgc agcgccgttg ggcaaatata acagcaatcc aacattgcgt
    30361 aactttgccg caaaccgttt cggtccatag gcttccagca cctgcacagc aggtaagttc
    30421 agcgagcgca ccagcgcctc gctcatgttg atcgggccat gaaaaccgct atcaaagtta
    30481 cctagtcgat aatcaccggt gcgccggggg acgtcttgca gcagtgatgc cgggtggatc
    30541 aagccttcat ccagcgccag accataaaca aacggtttga gcaccgatcc tggcgatcgg
    30601 atcgcattga ccatatcaac atgaccaaag cgtgaatcat cgttgagatc aaccgatccc
    30661 acccagccgc gaacacgcat atcggtatga ttaaccacga tcatcgccag tgagctgcgc
    30721 ggtggcaacc gccctttcca gttttgcgcc agttcttcca gacgtcgttg aagaccggca
    30781 tccagcgtag taacgatttt gtcgcttttg cttttaccga gcatcatgcg cgaaaacagc
    30841 ggtgccagtt gcggcatttg tcggggtgcc agccagatgg gttcttccct tgactctttt
    30901 acctgctcac gggaccacac accttgcgca gccatccgtt cgagcacttt attacgcgta
    30961 ttacgcgcgg cttcggcacg ctccggccaa cgatccgggc gcagtcggct gggcgcttgc
    31021 ggtaacaccg ccagcattgc cgcctaggaa tagcttaaat tcgcaggcga ttttccgaga
    31081 taagcccaac ttgccgcacc gatcccctgc aacgtaccgc caaacggagc gcggttaaga
    31141 tacaagatca gaatttcacg cttagacaaa tgccactcca gttgcaacgc gcgccagagc
    31201 tggagaattt tgccaccaaa tgttttgggg tgaggatcaa gcagacgagc aacctgcata
    31261 gtgagcgtgc tgccaccgga aataacccgt cccgaagtga gatcttgcca tgctgcgcgc
    31321 gccaccgaga atggattaac ccccggatgc ttccagaacc agcgatcttc ataattgatc
    31381 agcgcttcaa ggtaacgtgc ccctattaag ttgtcaagca tgttatgacc cctgcggggt
    31441 ataaaaagtc ccgtcgcgca tcatggcgaa cagaacgtcg cagcgtcgtc tcgccagggc
    31501 gataagcacc tgattgtgtc gttttccctg actcattttg cgggtgtagt aag
//
LOCUS       DADLSA010000034        30139 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.034, whole genome shotgun sequence.
ACCESSION   DADLSA010000034 DADLSA010000000
VERSION     DADLSA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30139
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.034"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..70
                     /locus_tag="JN691_001566"
     CDS             <1..70
                     /locus_tag="JN691_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7243039.1"
                     /translation="LRFAIREGGRTVGAGVVAKVLS"
     gene            300..683
                     /gene="secE"
                     /locus_tag="JN691_001567"
     CDS             300..683
                     /gene="secE"
                     /locus_tag="JN691_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAY7243040.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            685..1230
                     /gene="nusG"
                     /locus_tag="JN691_001568"
     CDS             685..1230
                     /gene="nusG"
                     /locus_tag="JN691_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAY7243041.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1389..1817
                     /gene="rplK"
                     /locus_tag="JN691_001569"
     CDS             1389..1817
                     /gene="rplK"
                     /locus_tag="JN691_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAY7243042.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1821..2525
                     /gene="rplA"
                     /locus_tag="JN691_001570"
     CDS             1821..2525
                     /gene="rplA"
                     /locus_tag="JN691_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAY7243043.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2938..3435
                     /gene="rplJ"
                     /locus_tag="JN691_001571"
     CDS             2938..3435
                     /gene="rplJ"
                     /locus_tag="JN691_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAY7243044.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3502..3867
                     /gene="rplL"
                     /locus_tag="JN691_001572"
     CDS             3502..3867
                     /gene="rplL"
                     /locus_tag="JN691_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAY7243045.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4187..8215
                     /gene="rpoB"
                     /locus_tag="JN691_001573"
     CDS             4187..8215
                     /gene="rpoB"
                     /locus_tag="JN691_001573"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAY7243046.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8292..12515
                     /gene="rpoC"
                     /locus_tag="JN691_001574"
     CDS             8292..12515
                     /gene="rpoC"
                     /locus_tag="JN691_001574"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAY7243047.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12757..13890)
                     /gene="thiH"
                     /locus_tag="JN691_001575"
     CDS             complement(12757..13890)
                     /gene="thiH"
                     /locus_tag="JN691_001575"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAY7243048.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13887..14732)
                     /gene="thiG"
                     /locus_tag="JN691_001576"
     CDS             complement(13887..14732)
                     /gene="thiG"
                     /locus_tag="JN691_001576"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAY7243049.1"
                     /translation="MSSGRNISCRMATRSCFFRLLQGVEMLRIADKTFDSHLFTGTGK
                     FASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVALLPNTSGAKTAEE
                     AIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPV
                     LCKRLEEVGCAAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQA
                     LEMGADAVLVNTAIAVADDPVNMAKAFRLAVEAGLLARQSGPGSRSYFAHATSPLTGF
                     LEASA"
     gene            complement(14659..14859)
                     /gene="thiS"
                     /locus_tag="JN691_001577"
     CDS             complement(14659..14859)
                     /gene="thiS"
                     /locus_tag="JN691_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAY7243050.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14843..15598)
                     /gene="thiF"
                     /locus_tag="JN691_001578"
     CDS             complement(14843..15598)
                     /gene="thiF"
                     /locus_tag="JN691_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAY7243051.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15591..16226)
                     /gene="thiE"
                     /locus_tag="JN691_001579"
     CDS             complement(15591..16226)
                     /gene="thiE"
                     /locus_tag="JN691_001579"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY7243052.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16226..18121)
                     /gene="thiC"
                     /locus_tag="JN691_001580"
     CDS             complement(16226..18121)
                     /gene="thiC"
                     /locus_tag="JN691_001580"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAY7243053.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18202..18302)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18355..18831)
                     /gene="rsd"
                     /locus_tag="JN691_001581"
     CDS             complement(18355..18831)
                     /gene="rsd"
                     /locus_tag="JN691_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAY7243054.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18926..19699
                     /gene="nudC"
                     /locus_tag="JN691_001582"
     CDS             18926..19699
                     /gene="nudC"
                     /locus_tag="JN691_001582"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAY7243055.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19739..20803
                     /gene="hemE"
                     /locus_tag="JN691_001583"
     CDS             19739..20803
                     /gene="hemE"
                     /locus_tag="JN691_001583"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAY7243056.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20960..21631
                     /gene="nfi"
                     /locus_tag="JN691_001584"
     CDS             20960..21631
                     /gene="nfi"
                     /locus_tag="JN691_001584"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAY7243057.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21674..22264
                     /locus_tag="JN691_001585"
     CDS             21674..22264
                     /locus_tag="JN691_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAY7243058.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22451..22723
                     /gene="hupA"
                     /locus_tag="JN691_001586"
     CDS             22451..22723
                     /gene="hupA"
                     /locus_tag="JN691_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAY7243059.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22736..23431
                     /locus_tag="JN691_001587"
     CDS             22736..23431
                     /locus_tag="JN691_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAY7243060.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23433..23858)
                     /gene="zraP"
                     /locus_tag="JN691_001588"
     CDS             complement(23433..23858)
                     /gene="zraP"
                     /locus_tag="JN691_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAY7243061.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24096..25472
                     /gene="zraS"
                     /locus_tag="JN691_001589"
     CDS             24096..25472
                     /gene="zraS"
                     /locus_tag="JN691_001589"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAY7243062.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25469..26794
                     /gene="zraR"
                     /locus_tag="JN691_001590"
     CDS             25469..26794
                     /gene="zraR"
                     /locus_tag="JN691_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HAY7243063.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCATLNESLLESELFGHEKGAF
                     AGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQVMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26791..28080)
                     /gene="purD"
                     /locus_tag="JN691_001591"
     CDS             complement(26791..28080)
                     /gene="purD"
                     /locus_tag="JN691_001591"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAY7243064.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28092..29681)
                     /gene="purH"
                     /locus_tag="JN691_001592"
     CDS             complement(28092..29681)
                     /gene="purH"
                     /locus_tag="JN691_001592"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAY7243065.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7057 a         7859 c         8291 g         6932 t
ORIGIN      
        1 tctgcgtttc gcaatccgtg aaggcggccg taccgttggc gcgggcgttg ttgctaaagt
       61 tctgagctaa ttgccgataa catttgacgc aatgcgcact aaaagggcat catttgatgc
      121 cctttttgca cgctttcgta ccagaacctg gctcatcagt gattttcttt gtcataatca
      181 ttgctgagac aggctctgtt gagggcgtat aatccgaaaa gctaatacgc gtttcgattt
      241 ggtttgcctc gcgatcgcgg ggtgaaaatg tttgtagaaa acttctgaca ggttggttta
      301 tgagtgcgaa taccgaagct caaggaagcg ggcgcggcct ggaagcgatg aagtgggtcg
      361 ttgtggtggc attgctcctg gtggcgattg tcggcaacta cctttatcgc gacattatgc
      421 tgccgctgcg tgcgctggcc gtagtaattc tgattgctgc agcgggtggt gtcgcgctgt
      481 taacgacaaa aggtaaagct accgttgctt ttgcccgtga agcgcgtacc gaagtccgta
      541 aggtcatttg gccgactcgc caggaaacat tgcacaccac gctgattgtg gctgcggtta
      601 ccgcagtaat gtcactgatc ctgtggggac tggatggtat tctggttcgc ctggtatcct
      661 ttatcactgg cctgaggttc tgagatgtct gaagctccta aaaagcgctg gtacgtcgtt
      721 caggcgtttt ccggttttga aggccgcgta gcaacgtcgc tgcgtgagca tatcaaatta
      781 cacaacatgg aagatttgtt tggtgaagtc atggtaccaa ccgaagaagt ggttgaaatc
      841 cgtggcggtc agcgtcgcaa aagcgaacgt aaattcttcc ctggctacgt cctcgttcag
      901 atggtgatga acgacgcgag ctggcacttg gtgcgcagcg taccgcgtgt gatgggcttc
      961 atcggcggta cttccgatcg tcctgcgcca atcagcgata aagaagtcga tgcgattatg
     1021 aaccgcctgc agcaggttgg tgataagccg cgtccgaaaa cgctgtttga accgggtgaa
     1081 atggtccgtg ttaatgatgg tccgttcgct gacttcaacg gtgttgttga agaagtggat
     1141 tacgagaaat ctcgtctgaa agtgtctgtt tctatcttcg gtcgtgcgac cccggtagag
     1201 ctggacttca gccaggttga aaaagcctaa cccagcgatc aaaaaagcgg cgatttaatc
     1261 gttgcacaag gcgtgagatt ggaatacaat ttcgcgcctt ttgtttttat gggccttgcc
     1321 cgtaaaacga ttttttatat cacggggagc ctctcagagg cgttattacc caacttgagg
     1381 aatttataat ggctaagaaa gtacaagcct atgtcaagct gcaggttgca gctggtatgg
     1441 ctaacccgag tccgccagta ggtccggctc tgggtcagca gggcgtaaac atcatggaat
     1501 tctgcaaagc gttcaacgca aaaactgatt ccatcgaaaa aggtctgccg attccggtag
     1561 taatcaccgt ttacgctgac cgttctttca ctttcgttac caagaccccg ccggcagcag
     1621 ttctgctgaa aaaagcggct ggtatcaagt ctggttccgg taagccgaac aaagacaaag
     1681 tgggtaaaat ttcccgcgct cagctgcagg aaatcgcgca gaccaaagct gccgacatga
     1741 ctggtgccga cattgaagca atgactcgct ccatcgaagg tactgcacgt tccatgggcc
     1801 tggtagtgga ggactaagaa atggctaaac tgaccaagcg catgcgtgtt atccgcgaga
     1861 aagttgatgc aaccaaacag tacgacatca acgaagctat cgcactgctg aaagagctgg
     1921 cgactgctaa attcgtagaa agcgtggacg tagctgttaa cctcggcatc gacgctcgta
     1981 aatctgacca gaacgtacgt ggtgcaactg tactgccgca cggtactggc cgttccgttc
     2041 gcgtagccgt atttacccaa ggtgcaaacg ctgaagctgc taaagctgca ggcgcagaac
     2101 tggtaggtat ggaagatctg gctgaccaga tcaagaaagg cgaaatgaac tttgacgttg
     2161 ttattgcttc tccggatgca atgcgcgttg ttggccagct gggccaggtt ctgggtccgc
     2221 gcggcctgat gccaaacccg aaagtgggta ctgtaacacc gaacgttgct gaagcggtta
     2281 aaaacgctaa agctggccag gttcgttacc gtaacgacaa aaacggcatc atccacacca
     2341 ccatcggtaa agtggacttt gacgctgaca aactgaaaga aaacctggaa gctctgctgg
     2401 ttgcgctgaa aaaagcaaaa ccgactcagg cgaaaggcgt gtacatcaag aaagttagca
     2461 tctccaccac catgggtgca ggtgttgcag ttgaccaggc tggcctgagc gcttctgtaa
     2521 actaatgcct ttacgtggga ggtgattttg tctacaatct tacccccacg tataatgctt
     2581 aatgcagacg tatatccgag atattcgggt tgtggcaagg cggcaactga gtgagtcgcc
     2641 agtcacatag ctaactatgt gactggtgcg aatgaaggaa gccaacgccg ccacaagttg
     2701 aatagcgacg gatagaaaag atttgttcgt tggagcctgg cctatccagg cctccgtcga
     2761 agaccgcagg agtttcgcaa gaaacttaat cccctgcgta gacggtgaca gaacgctaag
     2821 attattcttt tatattctgg cttgtttctg ctcaccgtaa ttaagacgct ctctccgttt
     2881 ggaggagtga agtgagttcc agagattttc tctggcaaac atccaggagc aaagctaatg
     2941 gctttaaatc ttcaagacaa acaagcgatt gttgctgaag tcagcgaagt agccaaaggc
     3001 gcgctgtctg cagtagttgc ggattcccgt ggcgtaactg tagataaaat gactgaactg
     3061 cgtaaagcag gtcgcgaagc tggcgtatac atgcgtgttg ttcgtaacac cctgctgcgc
     3121 cgtgctgttg aaggtactcc gttcgagtgc ctgaaagacg cgtttgttgg tccgaccctg
     3181 attgcatact ctatggaaca cccgggcgct gctgctcgtc tgttcaaaga gttcgcgaaa
     3241 gcgaatgcaa aatttgaggt caaagccgct gcctttgaag gtgagctgat cccggcgtct
     3301 cagatcgacc gcctggcaac tctgccgacc tacgaagaag caattgcacg cctgatggca
     3361 accatgaaag aagcttcggc tggcaaactg gttcgtactc tggctgctgt acgcgatgcg
     3421 aaagaagctg cttaatcgca gttatctttt taacgcattc gcttacgtat aaacttattc
     3481 tgatattcag gaacaattta aatgtctatc actaaagatc aaatcattga agcagttgca
     3541 gctatgtctg taatggacgt tgtagaactg atctctgcaa tggaagaaaa attcggtgtt
     3601 tccgctgctg ctgctgtagc tgtagctgct ggcccggttg aagctgctga agaaaaaact
     3661 gaattcgacg taattctgaa agctgctggc gctaacaaag ttgctgttat caaagcagta
     3721 cgtggcgcaa ctggcctggg tctgaaagaa gctaaagacc tggtagaatc tgcaccggct
     3781 gctctgaaag aaggcgtgag caaagacgac gcagaagcac tgaaaaaagc tctggaagaa
     3841 gctggcgctg aagttgaagt taaataagcc aacccttccg gttgcagcct gagaaatcag
     3901 gctgatggct ggtgactttt tagtcaccag cctttttgcg ctgtaaggcg ccagtagcgt
     3961 ttcacactgt ttgactactg ctgtgccttt caatgcttgt ttctatcgac gacttaatat
     4021 actgcgacag gacgtccgtt ctgtgtaaat cgcaatgaaa tggtttaagc gtgatagcaa
     4081 caggcattgc ggaaagtgtt ccattttccg gtcaacaaaa tagtgttgca caaactgtcc
     4141 gctcaatgga cagatgggtc gacttgtcag cgagctgagg aaccctatgg tttactccta
     4201 taccgagaaa aaacgtattc gtaaggattt tggtaaacgt ccacaagttc tggatgtacc
     4261 ttatctcctt tctatccagc ttgactcgtt tcagaaattt atcgagcaag atcctgaagg
     4321 gcagtatggt ctggaagctg ctttccgttc cgtattcccg attcagagct acagcggtaa
     4381 ttccgagctg caatacgtca gctaccgcct tggcgaaccg gtgtttgacg tccaggaatg
     4441 tcaaatccgt ggcgtgacct attccgcacc gctgcgcgtt aaactgcgtc tggtgatcta
     4501 tgagcgcgaa gcgccggaag gcaccgtaaa agacattaaa gaacaagaag tctacatggg
     4561 cgaaattccg ctcatgacag acaacggtac ctttgttatc aacggtactg agcgtgttat
     4621 cgtttcccag ctgcaccgta gtccgggcgt cttctttgac tccgacaaag gtaaaaccca
     4681 ctcttcgggt aaagtgctgt ataacgcgcg catcatccct taccgtggtt cctggctgga
     4741 cttcgaattc gatccgaagg acaacctgtt cgtacgtatc gaccgtcgcc gtaaactgcc
     4801 tgcgaccatc attctgcgcg ccctgaacta caccacagag cagatcctcg acctgttctt
     4861 tgaaaaagtt atctttgaaa tccgtgataa caagctgcag atggaactgg tgccggaacg
     4921 cctgcgtggt gaaaccgcat cttttgacat cgaagctaac ggtaaagtgt acgtagaaaa
     4981 aggccgccgt atcactgcgc gccacattcg ccagctggaa aaagacgacg tcaaactgat
     5041 cgaagtcccg gttgagtaca tcgcaggtaa agtggttgct aaagactata ttgatgagtc
     5101 taccggcgag ctgatctgcg cagcgaacat ggagctgagc ctggatctgc tggctaagct
     5161 gagccagtct ggtcacaagc gtatcgaaac gctgttcacc aacgatctgg atcacggccc
     5221 atatatctct gaaaccttac gtgtcgaccc aactaacgac cgtctgagcg cactggtaga
     5281 aatctaccgc atgatgcgcc ctggcgagcc gccgactcgt gaagcagctg aaagcctgtt
     5341 cgagaacctg ttcttctccg aagaccgtta tgacttgtct gcggttggtc gtatgaagtt
     5401 caaccgttct ctgctgcgcg aagaaatcga aggttccggt attctgagca aagacgacat
     5461 cattgatgtt atgaaaaagc tcatcgatat ccgtaacggt aaaggcgaag tcgatgatat
     5521 cgaccacctc ggcaaccgtc gtatccgttc cgttggcgaa atggcggaaa accagttccg
     5581 cgttggcctg gtacgtgtag agcgtgcggt gaaagagcgt ctgtctctgg gcgatctgga
     5641 taccctgatg cctcaggata tgatcaacgc caagccgatt tccgcagcag tgaaagagtt
     5701 cttcggttcc agccagctgt ctcagtttat ggaccagaac aacccgctgt ctgagattac
     5761 gcacaaacgt cgtatctccg cactcggccc aggcggtctg acccgtgaac gtgcaggctt
     5821 cgaagttcga gacgtacacc cgactcacta cggtcgcgta tgtccaatcg aaacccctga
     5881 aggtccgaac atcggtctga tcaactctct gtccgtgtac gcacagacta acgaatacgg
     5941 cttccttgag actccgtatc gtaaagtgac cgacggtgtt gtaactgacg aaattcacta
     6001 cctgtctgct atcgaagaag gcaactacgt tatcgcccag gcgaactcca acctggatga
     6061 agaaggccac ttcgtagaag acctggtaac ttgccgtagc aaaggcgaat ccagcttgtt
     6121 cagccgcgac caggttgact acatggacgt atccacccag caggtggtat ccgtcggtgc
     6181 gtccctgatc ccgttcctgg aacacgatga cgccaaccgt gcattgatgg gtgcgaacat
     6241 gcaacgtcag gccgttccga ctctgcgcgc tgataagccg ctggttggta ctggtatgga
     6301 acgtgctgtt gccgttgact ccggtgtaac tgcggtagct aaacgtggtg gtgtcgttca
     6361 gtacgtggat gcttcccgta tcgttatcaa agttaacgaa gacgagatgt atccgggtga
     6421 agcaggtatc gacatctaca acctgaccaa atacacccgt tctaaccaga acacctgtat
     6481 caaccagatg ccgtgtgtgt ctctgggtga accggttgaa cgtggcgacg tgctggcaga
     6541 cggtccgtcc accgacctcg gtgaactggc gcttggtcag aacatgcgcg tagcgttcat
     6601 gccgtggaat ggttacaact tcgaagactc catcctcgta tccgagcgtg ttgttcagga
     6661 agaccgtttc accaccatcc acattcagga actggcgtgt gtgtcccgtg acaccaagct
     6721 gggtccggaa gagatcaccg ctgacatccc gaacgtgggt gaagctgcgc tctccaaact
     6781 ggatgaatcc ggtatcgttt acattggtgc ggaagtgacc ggtggcgaca ttctggttgg
     6841 taaggtaacg ccgaaaggtg aaactcagct gaccccagaa gaaaaactgc tgcgtgcgat
     6901 cttcggtgag aaagcctctg acgttaaaga ctcttctctg cgcgtaccaa acggtgtatc
     6961 cggtacggtt atcgacgttc aggtctttac tcgcgatggc gtagaaaaag acaaacgtgc
     7021 gctggaaatc gaagaaatgc agctcaaaca ggcgaagaaa gacctgtctg aagaactgca
     7081 gatcctcgaa gcgggtctgt tcagccgtat ccgtgctgtg ctggtagccg gtggcgttga
     7141 agctgagaag ctcgacaaac tgccgcgcga tcgctggctg gagctgggcc tgacagacga
     7201 agagaaacaa aatcagctgg aacagctggc tgagcagtat gacgaactga aacacgagtt
     7261 cgagaagaaa ctcgaagcga aacgccgcaa aatcacccag ggcgacgatc tggcaccggg
     7321 cgtgctgaag attgttaagg tatatctggc ggttaaacgc cgtatccagc ctggtgacaa
     7381 gatggcaggt cgtcacggta acaagggtgt aatttctaag atcaacccga tcgaagatat
     7441 gccttacgat gaaaacggta cgccggtaga catcgtactg aacccgctgg gcgtaccgtc
     7501 tcgtatgaac atcggtcaga tcctcgaaac ccacctgggt atggctgcga aaggtatcgg
     7561 cgacaagatc aacgccatgc tgaaacagca gcaagaagtc gcgaaactgc gcgaattcat
     7621 ccagcgtgcg tacgatctgg gcgctgacgt tcgtcagaaa gttgacctga gtaccttcag
     7681 cgatgaagaa gttatgcgtc tggctgaaaa cctgcgcaaa ggtatgccaa tcgcaacgcc
     7741 ggtgttcgac ggtgcgaaag aagcagaaat taaagagctg ctgaaacttg gcgacctgcc
     7801 gacttccggt cagatccgcc tgtacgatgg tcgcactggt gaacagttcg agcgtccggt
     7861 aaccgttggt tacatgtaca tgctgaaact gaaccacctg gtcgacgaca agatgcacgc
     7921 gcgttccacc ggttcttaca gcctggttac tcagcagccg ctgggtggta aggcacagtt
     7981 cggtggtcag cgtttcgggg agatggaagt gtgggcgctg gaagcatacg gcgcagcata
     8041 caccctgcag gaaatgctca ctgttaagtc tgatgacgtg aacggtcgta ccaagatgta
     8101 taaaaacatc gtggacggca accatcagat ggagccgggc atgccagaat ccttcaacgt
     8161 attgttgaaa gagattcgtt cgctgggtat caacatcgaa ctggaagacg agtaattctc
     8221 gctcaaacag gtcactgctg tcggggtaaa acccggcagc ggattgtgct aactccgacg
     8281 ggagcaaatc cgtgaaagat ttattaaagt ttctgaaagc gcagactaaa accgaagagt
     8341 ttgatgcgat caaaattgct ctggcttcgc cagacatgat ccgttcatgg tctttcggtg
     8401 aagttaaaaa gccggaaacc atcaactacc gtacgttcaa accagaacgt gacggccttt
     8461 tctgcgcccg tatctttggg ccggtaaaag attacgagtg cctgtgcggt aagtacaagc
     8521 gcctgaaaca ccgtggcgtc atctgtgaga agtgcggcgt tgaagtgacc cagactaaag
     8581 tacgccgtga gcgtatgggc cacatcgaac tggcttcccc gactgcgcac atctggttcc
     8641 tgaaatcgct gccgtcccgt atcggtctgc tgctcgatat gccgctgcgc gatatcgaac
     8701 gcgtactgta ctttgaatcc tatgtggtta tcgaaggcgg tatgaccaac ctggaacgtc
     8761 agcagatcct gactgaagag cagtatctgg acgcgctgga agagttcggt gacgaattcg
     8821 acgcgaagat gggtgcggaa gcaatccagg ctctgctgaa gagcatggat ctggagcaag
     8881 agtgcgaaca gctgcgtgaa gagctgaacg aaaccaactc cgaaaccaag cgtaaaaagc
     8941 tgaccaagcg tatcaaactg ctggaagcgt tcgttcagtc tggtaacaaa ccagagtgga
     9001 tgatcctgac cgttctgccg gtactgccgc cagatctgcg tccgctggtt ccgctggatg
     9061 gtggtcgttt cgcgacttct gacctgaacg atctgtatcg tcgcgtcatt aaccgtaaca
     9121 accgtctgaa acgtctgctg gatctggctg cgccggacat catcgtacgt aacgaaaaac
     9181 gtatgctgca ggaagcggta gacgccctgc tggataacgg tcgtcgcggt cgtgcgatca
     9241 ccggttctaa caagcgtcct ctgaaatctt tggccgacat gatcaaaggt aaacagggtc
     9301 gtttccgtca gaacctgctc ggtaagcgtg ttgactactc cggtcgttct gtaatcaccg
     9361 taggtccata cctgcgtctg catcagtgcg gtctgccgaa gaaaatggcg ctggagctgt
     9421 tcaaaccgtt catctacggc aagctggaac tgcgtggtct tgctaccacc attaaagctg
     9481 cgaagaaaat ggttgagcgc gaagaagctg tcgtttggga tatcctggac gaagttatcc
     9541 gcgaacaccc ggtactgctg aaccgtgcac cgactctgca ccgtctgggt atccaggcat
     9601 ttgaaccggt actgatcgaa ggtaaagcta tccagctgca cccgctggtt tgtgcggcat
     9661 ataacgccga cttcgatggt gaccagatgg ctgttcacgt accgctgacg ctggaagccc
     9721 agctggaagc gcgtgcgctg atgatgtcta ccaacaacat cctgtccccg gcgaacggcg
     9781 aaccaatcat cgttccgtct caggacgttg tactgggtct gtactacatg acccgtgact
     9841 gtgttaacgc caaaggcgaa ggcatggtgc tgactggccc gaaagaagca gaacgtctgt
     9901 atcgctctgg tctggcttct ctgcatgcgc gcgttaaagt gcgtatcacc gagtatgaaa
     9961 aagatgctaa cggtgaatta gtagcgaaaa ccagcctgaa agacacgact gttggccgtg
    10021 ccattctgtg gatgattgta ccgaaaggtc tgccttactc catcgtcaac caggcgctgg
    10081 gtaaaaaagc aatctccaaa atgctgaaca cctgctaccg cattctcggt ctgaaaccga
    10141 ccgttatttt tgcggaccag atcatgtaca ccggcttcgc ctatgcagcg cgttctggtg
    10201 catctgttgg catcgatgac atggtcatcc cggagaagaa acacgaaatc atctccgagg
    10261 cagaagcaga agttgctgaa attcaggagc agttccagtc tggtctggta actgcgggcg
    10321 aacgctacaa caaagttatc gatatctggg ctgcggcgaa cgatcgtgta tccaaagcga
    10381 tgatggataa cctgcaaact gaaaccgtta ttaaccgtga cggtcaggaa gagaagcagg
    10441 tttccttcaa cagcatctac atgatggccg actccggtgc gcgtggttct gcggcacaga
    10501 ttcgtcagct tgctggtatg cgtggtctga tggcgaagcc ggatggctcc atcatcgaaa
    10561 cgccaatcac cgcgaacttc cgtgaaggtc tgaacgtact ccagtacttc atctccaccc
    10621 acggtgctcg taaaggtctg gcggataccg cactgaaaac tgcgaactcc ggttacctga
    10681 ctcgtcgtct ggttgacgtg gcgcaggacc tagtggttac cgaagacgat tgtggtaccc
    10741 atgaaggtat catgatgact ccggttatcg agggtggtga cgttaaagag ccgctgcgcg
    10801 atcgcgtact gggtcgtgta actgctgaag acgttctgaa gccgggtact gctgatatcc
    10861 tcgttccgcg caacacgctg ctgcacgaac agtggtgtga cctgctggaa gagaactctg
    10921 tcgacgcggt aaaagtacgt tctgttgtat cttgtgacac cgactttggt gtatgtgcgc
    10981 actgctacgg tcgtgacctg gcgcgtggcc acatcatcaa caaaggtgaa gcaatcggtg
    11041 ttatcgcggc acagtccatc ggtgaaccgg gtacacagct gaccatgcgt acgttccaca
    11101 tcggtggtgc ggcatctcgt gcggctgctg aatccagcat ccaggtgaaa aacaaaggta
    11161 gcatcaagct cagcaacgtg aagtcggttg tgaactccag cggtaaactg gttatcactt
    11221 cccgtaacac cgaactgaaa ctgatcgacg aattcggtcg taccaaagaa agctacaaag
    11281 tgccttacgg tgcggtactg gcgaaaggcg atggcgaaca ggttgctggc ggcgaaaccg
    11341 ttgcaaactg ggacccgcac accatgccgg ttatcaccga agtaagcggt tttgtacgct
    11401 ttactgacat gatcgacggc cagaccatta ctcgtcagac cgacgaactg accggtctgt
    11461 cttcgctggt ggttctggat tccgcagaac gtaccgcagg tggtaaagat ctgcgtccgg
    11521 cactgaaaat cgttgatgct cagggtaacg acgttctgat cccaggtacc gatatgcctg
    11581 cgcagtactt cctgccgggt aaagcgattg ttcagctgga agatggcgta cagatcagct
    11641 ctggtgacac cctggcgcgt attccgcagg aatccggcgg taccaaggac atcaccggtg
    11701 gtctgccgcg cgttgcggac ctgttcgaag ctcgtcgtcc gaaagagccg gcaatcctgg
    11761 ctgaaatcag cggtatcgtt tccttcggta aagaaaccaa aggtaaacgt cgtctggtta
    11821 tcaccccggt agacggtagc gatccgtacg aagagatgat tccgaaatgg cgtcagctca
    11881 acgtgttcga aggtgaacgt gtagaacgtg gtgacgtaat ttccgacggt ccggaagcgc
    11941 cgcacgacat tctgcgtctg cgtggtgttc atgctgtgac tcgttacatc gttaacgaag
    12001 tacaggacgt ataccgtctg cagggcgtta agattaacga taaacacatc gaagttatcg
    12061 ttcgtcagat gctgcgtaaa gctaccatcg ttaacgcggg cagctccgac ttcctggaag
    12121 gcgaacaggt tgaatactct cgcgtcaaga tcgcaaaccg cgaactggaa gcgaacggca
    12181 aagtgggtgc aacttactcc cgcgatctgc tgggtatcac caaagcgtct ctggcaaccg
    12241 agtccttcat ctccgcggca tcgttccagg agaccactcg cgtgctgacc gaagcagccg
    12301 ttgcgggcaa acgcgacgaa ctgcgcggcc tgaaagagaa cgttatcgtg ggtcgtctga
    12361 tcccggcagg taccggttac gcgtaccacc aggatcgtat gcgtcgccgt gctgcgggtg
    12421 aagctccggc tgcaccgcag gtgactgcag aagacgcatc tgccagcctg gcagaactgc
    12481 tgaacgcagg tctgggcggt tctgataacg agtaatcgtt aatccgcaaa taacgtaaaa
    12541 acccgcttcg gcgggttttt ttatgggtgt tattcagagg gcaaattctt cacggaaaat
    12601 attaagaaag gcagggcgaa acgaggaaga agcttttcgc cctggacaac attcctgctg
    12661 acggcactac cataaaaaaa cacaacgctt tcaacaacct tgcgtttttc atcttccgta
    12721 gcggctcgga ataaatttac gttcttacat tccgtctcat agtctttgcg aggcgcgtcc
    12781 cagatagctg tcccagtctt tccataccgg ctgcaaaccc tgagcggtta acgcggcagc
    12841 aaccgcttcc ggtctgcgat cgtcgtgcgg tgagaactgt tccaactcgg ggtgattatc
    12901 ggcatagcca cctggctgcg ttttcgagaa ggcgctgacg ttattgatcg ccagcggaat
    12961 aacgcgatcg cgaaaccacg gtgattcccg cgtggagagt gacagttcaa tctccggtgc
    13021 aagcagtcgg aaggcgcaga tggtttgcac taactggcgt tcatccataa tcgacgcagg
    13081 ctcaatgccg ccagtacacg ggcgcaggcg cggaaaggag acagagtaac ggctttgcca
    13141 gtaatgctgt tgcagccata gcaaatgttc ggcaaccata tagcagtcaa cgcgccagtt
    13201 gtcggaaagg ccaattagcg cgccgaggcc tatcttatca atccccgcac gccccagccg
    13261 atccggcgtt tccagccgcc agaagaagtc ctgttttttg cctttcaaat gatggcgggt
    13321 ataagtcgcc tcgtgatatg tctcctgata aaccatcacc ccatccagac caagttgctt
    13381 taactcggcg tattccgtct ccgccagcgg ttgcacttcc atttgtagtg aagagaactg
    13441 ttcacgcagg gcggggagat gacgacgaaa gtaatccatc cccactttcg cctgatgttc
    13501 accagtgact aatagcagat gttcaaagcc catctcccgt atagcggcgc tttccctggc
    13561 aatatccgct tcatccagcg ttttgcgctt gatgcgatta ctcatggaaa atccgcagta
    13621 cgtgcagtcg ttagcgcaaa gattggaaag ataaagcggg acgtagaaac taactgtgtt
    13681 gccaaatcgc tgacgggtca gacgctgcgc ctgttgggcc agttgttcca gatagccact
    13741 ggcggcaggc gataacagcg ccatcatgtc gtcgcgggtg agttgcgagg catttagcgc
    13801 ccgctctacg tcagcagccg ttttgccgtt gatacgcagg cggatgtcgt cccagtccag
    13861 ttgtcgccag cgattgctga aggttttcat gccgatgcct ccagaaatcc ggtcagcggg
    13921 ctggtggcat gagcaaaata actgcggctg cccggtccgg actgacgtgc cagcaggcct
    13981 gcttctaccg ccagacgaaa tgccttcgcc atgttgacgg gatcgtccgc gacggcaatc
    14041 gccgtattca ctaacaccgc gtcggccccc atttccagcg cctgcgcggc atggctggga
    14101 acgccgatgc cagcatcgac aaccaccgga actgtggcct gctggataat aatctccagc
    14161 atggcacggg tttccagtcc ctgattcgag ccaatcggcg cgccgagcgg catcaccgct
    14221 gcacagccga cttcttccag acgtttacac aataccggat cggccccgca gtaaggcagc
    14281 acgacaaatc cctgttgtac cagcgtttcg gcggctttca gggtttcgat gggatcgggc
    14341 aacagccagc gggcgtcagg gtgaatctcc aattttagcc agtttgtgcc taacgcttca
    14401 cgtgccagat gggcggcgaa aatggcttct tccgctgttt tcgccccgga agtattcggc
    14461 agcagggcca cacccgccgc gataagcggt tcgaggatag cgtcgttgtg ctggcgcaag
    14521 tcgacacgtt tcatcgccag tgtcaccagc tggctgccgg aagcgcggat cgcctccacc
    14581 atcagttgtg aagaagcgaa tttccctgtg ccggtaaaca gatgtgaatc aaacgttttg
    14641 tccgcaatac gtaacatctc aaccccctgc aataacctga aaaagcagga tctggtcgcc
    14701 atcctgcacg atatgttgcg tccactgctc acgcgggacg atttgctgat taatcgccag
    14761 agccgcgccc gcttgtcgtt ggtcgagttg ctccagtagt tcgtgaacag tttgcccggc
    14821 ggcgcactgc atcggttgat cgttaaacag gatctgcatt gcttcctccg cataccgggc
    14881 aaccactggc gcggcgcaac gccaggctgc gccactggct cgacttaccg tcgaacagtc
    14941 ggagttctcc cgcaggcgtc tctataccgc ttaataactt aatggcttcc agtgcctgca
    15001 aagtgcccat caccccgacc accgggccaa ccacgcccgc cgtgcggcag ttgcgttctg
    15061 gctgctggtt atctggccac aggcagcggt aacacccctg ctcccagggc ggtgtcagta
    15121 ccatcaactg accgccaaat ccgaccgcgc tggcggtgat aagcggcgtg ttgagtgcca
    15181 cgcaggcggc attaatctcc tggcgagtcg ccatattgtc ggtacagtcg agcaccacat
    15241 cggcttgtgc aaccgcatct ttcaacgcct cacccgttaa ccgttgttgt aatgctgtca
    15301 gttgaatgtc gggattcaac tgtgtcagtc gctgttggct gacctgcgat ttcgggctgt
    15361 tgatatcttc agtggtaaag aggatttgtc gttgcagatt gcttaaatgc acatcgtcgt
    15421 catctgccag taccagcgtc ccgacgccag cccctgccag gtatagcgca gcaggtgtac
    15481 ccagcccgcc aagaccaata atcagcacct ggctgtcgag cagtttttgc tgcccgtcaa
    15541 gagcgatatc gtcgagcaga atttggcggc tataacgcat aaagtcacgg tcattcatcg
    15601 ccaactcctg caatttccag caactgtgcc gttgccaaac gccagtctgc ggcttgagta
    15661 atggcgctga cgacggcgat actgccgaca cccgttgcta tcaccgcagg cgcgcgtgcc
    15721 agactgatac cgccaatcgc cacggtggga taatccgcca ggcgctcaac atgccgtgcc
    15781 aactgttcca gcccctgcgg tgcagaaggc atctgtttgg tttgcgtcgg gaacacatgt
    15841 cccagcgcga tataagaggg gcgtgctgcc agcgcgacgt cgatttccat atcgtcatgt
    15901 gtcgaaacgc ccagccgcag gcctgccgcg cggatggcat tgagatcggt ggcttgcaaa
    15961 tcttcctgcc ccaaatggac gccatacgcc tgatgcttga tcgcgagccg ccagtaatcg
    16021 ttgataaaca atcgcgcgtt atagcggcgg cccagcgcaa ttgccgccac gacatcggct
    16081 tccacctctt catcgcgccg atctttgatg cgtagctgga gagtacgtac gcctgcatcc
    16141 aacagacgtt cgatccactg tacgctgtcc accaccgggt acagtcctaa acgaaaaggt
    16201 acaggaggaa aatcaggctg atacatcacg cttcctcctt atgcaggtag atttctccgc
    16261 ctctggcacg gaagttctcc gacatatccg ccattcccac ttcaatggtt tgcgcggcgg
    16321 cgtaatcgcg tacttcctgg ctgattttca tcgagcagaa ttttggccca cacatggagc
    16381 aaaaatgggc gactttgccg gactcttggg gcagggtttc atcgtgataa gcgcgggcgg
    16441 taaacgggtc gagggccaga ttaaactggt cttcccagcg aaattcgaag cgggctttcg
    16501 acatggcgtt atcgcgaatt tgcgcgcccg gatgcccttt tgccaggtca gcagcgtggg
    16561 cggcaatctt ataagtgata agcccctgct taacatcttc tttattgggc agacccagat
    16621 gctcttttgg cgttacgtaa cagagcatcg cgcagccaaa ccagccaatc atcgccgcac
    16681 caatccccga cgtgaagtgg tcatagcccg gtgcaatatc ggtagttagc ggccccagag
    16741 tgtaaaacgg cgcttcgtgg cagtgctcta actcctcggt catattgcgg cggatcatct
    16801 gcatcggcac atggcctggg ccttcaatca tcacctgtac gtcatattcc caggcgattt
    16861 tggtcagttc gcccagcgta tgcaattcgg caaactgcgc ttcatcgttg gcgtcctgaa
    16921 tagaaccggg gcgcagaccg tcgcccagcg acagcgaaac gtcataagcg gcacagattt
    16981 cacaaatttc gcggaagtgt tgatagagga aattttcctg atgatgggag aggcaccatt
    17041 tcgccataat cgaaccgccg cgagagacga taccggtcag gcgtttcgcg gtcatcggca
    17101 catagcgcag caggacgccc gcatggatag tgaagtaatc cacaccttgc tcggcctgtt
    17161 ccagcagcgt gtcgcggaac gcttcccagg taagatcttc ggcgatcccg ttaaccttct
    17221 ccagcgcctg gtagatcggc actgtaccga tcggcaccgg actgttacgc aaaatccact
    17281 cgcgggtttc gtgaatatag cgaccggtgg agagatccat caccgtatcc gctccccagc
    17341 gcgtggacca taccagcttt tccacttctt cttcgatgga agaggtgacc gccgagttgc
    17401 cgatattggc gttaactttt accaggaagt tacggccaat aatcatcggc tccgattccg
    17461 gatgattaat gttggccggg ataatcgcac gtccggcagc aacttcatca cggacaaatt
    17521 ccgcagtgat attttccggc agatgtgcgc caaagctcat tcccggatgc tggtggcgta
    17581 aaacctcgct acggatgcgc tcgcggccca tattctcgcg gatggcgatg aattccattt
    17641 ccggcgtgat gatgccctgg cgggcgtagt gcagttgggt gacacggcgt cctgttttgg
    17701 cgcgttttgg cgttaataag ccgctaaaac gtagctcgtc gagaccatca tctgccagcc
    17761 gcgttctggt gtaatcggaa ctgcgcacgg taagttcttc ggtatcggcg cgcgcatcga
    17821 tccacggctg gcgtagtttt gccagccctt gctgcacgtt aatggcgatc tgcggatcac
    17881 catacgggcc ggaggtgtcg tagaccggaa tcgcttcgtt ttcttcgtac tgcggctgtt
    17941 ctttgctacc gccaattagc gtcgggctaa gctggatctc acgcatcggc acgcgcacgc
    18001 cggggtgtgt gccagtgata taaatgcgtt ttgagttggg aaaggcggtg ccttccaggg
    18061 tgtcgataaa atgttgggcc cgggcgcgtt gttcgcggcg ggtcagtttt gttgcagaca
    18121 tagctcattc caaaaagtta aggacgtggc ttgtcagacg acggatgaag caagagacga
    18181 tcgccgcaaa ggcgatgcga tagcagatta actcttgttc ccttcgcagg tattagcctg
    18241 atcaggttcc gcggatcccg aataaacggt ctcagccagt taaggcactc cgacaagaaa
    18301 ttagccccca caaacggggc gttgaatgta aattacgcgt taacagcgca gaactcaagc
    18361 aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc agcttatctt ccagcacaaa
    18421 gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc
    18481 atgatcgatg gcggtttcca ggctggaatc gtagtaatcc ataatctgtt gggtattggc
    18541 ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg
    18601 aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag taatcgacca ggctctgaca
    18661 aaaatcatca agggcttttt cgtttagcct catgtacgat tctttgccag gcttaatgcc
    18721 aaccagattg tagtaagcca cgagcagatg cttacgtaca tgtagccagc gatcaaccag
    18781 tttgttactt cctctgacgc gctccgtcag gttatcgagc tggttaagca tgattgactc
    18841 cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca
    18901 gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt gaaaaattag atcacggctg
    18961 gtgggtcgtc agccatgaac aaaaattatg gttgccgaag ggagaattgc catatggcga
    19021 agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa atcggcgaat ggcagggaga
    19081 acctgtttgg ttaatacaac agcagcggcg tcacgatatg gggtcggtac gtcaggtcat
    19141 tgatctcgat gttgggctgt ttcaactggc cggacgtggc gtgcaactgg cggagtttta
    19201 ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg tatccgagca aaaccgaatg
    19261 ggcgatgctg tgcagccatt gccgtgagcg ttactacccg caaatcgccc cctgcattat
    19321 tgttgccatc cgtcgcgatg attcgatcct cctcgcccag catacccgcc atcgtaacgg
    19381 tgtccataca gtacttgccg gattcgtcga agtgggcgaa accctcgagc aggcagtcgc
    19441 gcgggaagtg atggaagaga gcggaattaa agttaaaaac ttgcgttacg tgacttctca
    19501 gccgtggccg tttcctcagt ctttaatgac cgcgtttatg gcggaatatg acagcggcga
    19561 catcgtgatc gacccgaaag aattgctcga ggcgaactgg tatcgctatg acgatttgcc
    19621 gttactcccg ccgcccggca ccgtagcgcg ccgtctgata gaagatactg tggcgatgtg
    19681 tcgggcagag tatgagtgat gatacactga ccgcctgacg cactaaggaa cagccaaaat
    19741 gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac
    19801 tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc
    19861 ccaggcgggc gattttatgt cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt
    19921 gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc ttttccgata tcctcaccgt
    19981 gccggacgcg atggggttag ggctctattt tgaagccgga gaaggtccgc gttttacctc
    20041 gccagtcacc tgcaaagccg acgtcgataa actgccaatt ccggacccgg aagatgagct
    20101 gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct
    20161 gattggtttt tccggcagcc cgtggacgct ggcaacctac atggtggaag gcggcagcag
    20221 caaagccttc accgtgatca aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct
    20281 gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat gcgcagatta aagccggtgc
    20341 tcaggcagtg atgattttcg acacctgggg cggcgtgctt accgggcgcg attatcaaca
    20401 gttctcgctc tattacatgc ataaaattgt tgatggttta ctgcgtgaaa acgacggtcg
    20461 ccgcgtgcca gtcacgctgt ttaccaaagg cggcggacag tggctggaag ccatggctga
    20521 aaccggttgc gatgcgctgg gcctcgactg gacaacggac atcgccgatg cgcgccgccg
    20581 tgtgggcaat aaagtcgcgt tgcagggtaa tatggatccg tcgatgctgt acgcgccgcc
    20641 tgcccgcatt gaagaagaag tagcgactat acttgcaggt ttcggtcacg gcgaaggtca
    20701 tgtctttaac cttggtcacg gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt
    20761 cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc taagagcctg atccatcagg
    20821 ctattttatt tgccattttg aggccgggca gtgctcaaaa tcctcacgta ctacgtgtac
    20881 gctccggttt ttccgcgctg tccggcctca aactgccttc aacaattacg cctgttgaac
    20941 caggctctaa ggagcgatta tggatctcgc gtcattacgc gctcaacaaa tcgaactggc
    21001 ttcttctgtg atctgcgagg atcgactcga taaagatcca ccggatctga tcgccggagc
    21061 cgatgtcggg tttgagcagg gcggagaagt gacgcgagcg gcgatggtgc tgctgaaata
    21121 tccctcgctt gagctggtgg agtataaagt tgcccgcatc gccaccacca tgccttacat
    21181 tccaggtttt ctttccttcc gcgaatatcc tgcgctgctg gcagcgtggg agatgctgtc
    21241 gcaaaagccg gatttagtgt ttgtcgatgg tcatgggatc tcgcatcctc gccgtcttgg
    21301 cgtcgccagc cattttggct tattggtgga tgtgccgacc attggcgtgg cgaaaaaacg
    21361 gctctgcggt aaattcgaac cgctctccag cgaaccgggc gcgctggccc cactgatgga
    21421 taaaggcgag cagctggcct gggtctggcg cagcaaagcg cgctgtaacc cgttgtttat
    21481 cgctaccggc catcgggtca gcgtggacag cgcgctggcg tgggtacaac gctgcatgaa
    21541 aggctatcgt ctgccggagc caacgcgctg ggcggacgcg gtggcctcgg aacgtccggc
    21601 gttcgtgcgc tatacagcaa atcagcccta attcaggtaa actgcggata atttccgtat
    21661 ttgagaactc aacatgttac aaaacccaat tcatctgcgt ctggagcgcc tggaaagctg
    21721 gcagcatgtc actttcatgg cttgcttatg cgaacgcatg taccccaatt acgccatgtt
    21781 ctgccagcaa accggttttg gtgatgggca aatttaccgt cgtattctcg atctcatctg
    21841 ggaaacgctg accgttaaag acgcaaaagt aaatttcgac agccaactgg agaaatttga
    21901 agaagcgatt ccttcagccg acgatttcga tctgtacggc gtttatccgg caatcgatgc
    21961 ctgcgtggcg ttaagtgaac tggtccattc gcgtttgagt ggtgaaacgc tcgaacacgc
    22021 ggtggaagtg agtaagacct ccatcacgac cgttgcgatg ctggaaatga ctcaggctgg
    22081 tcgcgaaatg agcgatgaag agcttaaaga aaacccagct gtagagcaag aatgggacat
    22141 tcagtgggaa atattccgac ttttagctga gtgcgaagaa cgcgatatcg agctgataaa
    22201 aggccttagg gcagacctgc gtgagtcggg tgagagcaat attggtataa tttttcagca
    22261 ataagaccag aaaacgtgat ttaacgcctg atttgtcgta cctggagtct tccctttcgc
    22321 cccccgtctg gtctacattt ggggggcgaa aaaaagtggc tatcggtgcg tgtatgcagg
    22381 agagtgctat tctggcattt ccgtcgcact cgatgcttag caagcgataa acacattgta
    22441 aggataactt atgaacaaga ctcaactgat tgatgtaatt gcagagaaag cagaactgtc
    22501 caaaacccag gctaaagctg ctctggagtc cactctggct gcaattactg agtctctgaa
    22561 agaaggcgat gctgtacaac tggttggttt cggtaccttc aaagtgaacc accgcgctga
    22621 gcgtactggc cgcaacccgc agaccggtaa agaaatcaaa attgccgcag ctaacgtacc
    22681 ggcatttgtt tctggcaagg cactgaaaga cgcagttaag taagattgcg tggcagtgaa
    22741 cagttttaac gaaggggtgg tttcacccct tttgtctttc tggcgtcgat cattgatgct
    22801 ggcaggtgcg ctgtttctca ctgcctgtag ccacaactct tcacttcctc cctttaccgc
    22861 cagtggattt gctgaagacc agggcgcggt acgcatctgg cgaaaagaca gcggcgataa
    22921 tgtgcatctg cttgccgtgt ttagcccgtg gcgcagtggc gataccacga cgcgagagta
    22981 tcgctggcag ggcgataacc tcacgctcat caatatcaat gtttacagca aaccgccggt
    23041 gaacattcgc gcgcgttttg acgatcgcgg cgatctgagc tttatgcaac gtgaatccga
    23101 tggggaaaag cagcagcttt ctaacgacca aatcgattta taccgttatc gtgctgatca
    23161 gatccgccag attagcgatg ccttacgtca ggggagagtc gtgctgcgcc aggggcgctg
    23221 gcatgcgatg gaacagaccg tgaccacctg cgaagggcaa accattaaac ctgatttaga
    23281 ttcgcaggcg atagcgcata tcgagcgccg ccagagccgc tcttctgttg atgtcagcgt
    23341 ggcatggctg gaagcgcccg aaggttcgca attactgtta gtggcaaact ctgatttctg
    23401 tcgctggcaa cccaacgaga aaacgttctg atttaccagt ggcccatacc catatgaccg
    23461 ccaccaccgc agccgccgta gcccatgccc attccggctc cgcgcggaat acccgcttca
    23521 gccatcgcga tatctcgttt cacccgtaac tcatctaacg actgacgcaa attctccatc
    23581 tctttggcga ccgcgttaat tttgctgcta tccggtgggt tcgcggctaa cagggcattg
    23641 tattcataac gcttcgtcac cagttgctgt tgcagtgcgc tgctttgagc gtaaaagtca
    23701 ttatggattt tctgccacgc tgtctgttgt tcgctggtca aaggcgcggc attttgctgc
    23761 cacataccgt gtccgccgtg agcaaatgca gatgtcgatc ccatcgccat tgctgaaagc
    23821 gccatcatta ccagggcaat tttcgtgttc cgtttcatgg ttaatcctcc agtggttgtc
    23881 ttacttcggg tattgcatct tccgtgccaa cgatgaaacg ctgatatgac gggtaatctg
    23941 gcatgataaa cgagtaaaaa tgactcgcct gctgcgggta gcgagtcatt tttactcatt
    24001 gaaacttaag cctttgtgtt acagcgcagg gtaagcgctg ataaaagatg gcatgatttc
    24061 tgctgtcaga aagggatgag caggcaaaga agaagatgcg ttttatgcaa cgttctaaag
    24121 actccttagc taaatggtta agcgcgatcc tccccgtggt cattgttggg ctggtagggt
    24181 tgtttgcggt gacggtgatt cgcgattatg ggcgcgagac tgccgccgcc agacaaacgc
    24241 tgctggaaaa aggcagtgta cttattcgcg ctcttgaatc cggctcgcgc gtcggcatgg
    24301 ggatgcgcat gcatcatgcg cagcagcagg ccttactgga agaaatggcc gggcagcctg
    24361 gagtacgttg gtttgcggtc acggatgaac aaggaacaat cgtgatgcat agcaactccg
    24421 gcatggtggg aaaacagctt tattcccctc aggaaatgca gcagttacat ccgggagatg
    24481 aagaagcgtg gcggcggatc gatagcgcag acggcgagcc tgttctggaa atttatcgcc
    24541 agtttcaacc gatgtttgct gctggaatgc accggatgcg ccatatgcag cagtatgccg
    24601 cgacaccaca agcaattttc atcgctttcg acgccagtaa tattgtgagt gccgaagatc
    24661 gtgagcagag aaacaccctg attattctct tcgccctggc gacggtcttg ctggcaagcg
    24721 tattgtcatt cttctggtat cgccgctatc tgcgctcgcg ccagcttcta caagatgaaa
    24781 tgaagcgcaa agagaagctg gtggcgctgg ggcatcttgc ggcaggcgtt gcccacgaaa
    24841 tccgtaaccc actttcctcg attaaaggac tggcgaaata ctttgccgag cgcgcgcctg
    24901 cagggggaga agcgcatcaa ctggcgcagg tgatggcgaa agaggccgac cgtttaaacc
    24961 gcgtggtaag cgagttgctg gaactggtta agccaacgca tctggctttg caggcggtgg
    25021 atctcaacac gctgattaac cactcattac agctggtaag ccaggatgca aacagccggg
    25081 agatccagtt acgctttacc gccaacgaca cattaccgga aattcaggcc gatccggaca
    25141 ggctgactca ggtcctgttg aatctctatc tcaatgctat tcaggcgatt ggtcagcatg
    25201 gcgtgattag cgtgacggcc agcgaaagcg gcgcgggcgt gaaaatcagc gttaccgaca
    25261 gcggtaaggg aattgcggca gatcagcttg aagccatctt cactccgtac ttcaccacca
    25321 aagccgaagg caccggattg gggctggcgg tcgtgcataa tattgttgaa caacacggtg
    25381 gtacaattca ggtcgcaagc caggagggaa aaggctcaac gttcaccctc tggcttccgg
    25441 tcaatattac gcgtaaggac ccacaaggat gacgcacgat aatatcgata ttctggtggt
    25501 ggatgatgac attagccact gcactatttt gcaggcttta ctgcgcggct ggggctataa
    25561 cgtcgcgctg gcgaacagcg ggcgacaggc gctggagcag gtgcgggaac aggtttttga
    25621 tcttgtgctt tgcgatgtgc gaatggcgga gatggacggc atcgccacgc tgaaagagat
    25681 caaagcgtta aacccggcaa ttccggtgct gattatgact gcgtactcca gcgtcgagac
    25741 ggcggtagag gcactgaaaa ctggggcgct ggattatctc atcaagccgc tggatttcga
    25801 taacctacag gcgacgctgg aaaaggcgct cgcgcatacg cacagtattg atgctgaaac
    25861 acctgcggtg actgccagcc agttcggtat ggtcggtaaa agcccggcga tgcaacacct
    25921 gctcagtgaa atcgccctcg tcgcgccatc ggaagccacg gtgctgatcc acggcgattc
    25981 cggcaccggt aaagaactgg tcgctagggc gattcacgcc agtagcgcac gtagcgaaaa
    26041 accgctggta acgctcaact gtgcgacact caacgaatcc ttgctggaat ctgaattgtt
    26101 cggtcacgaa aaaggggcgt ttgctggagc cgataaacgc cgggaagggc gctttgttga
    26161 ggcggacggc ggcacgctgt ttctcgatga aattggcgat atctcgccga tgatgcaggt
    26221 gcgtctgctg cgtgcgattc aggagcgcga agttcagcgt gtcggtagca accagactat
    26281 ctcggttgat gtccggctga ttgcggcgac ccatcgcgat cttgccgcag aggtgaatgc
    26341 cgggcgtttt cgccaggatc tctactatcg cctgaatgtg gtggcgattg aagtaccatc
    26401 gctgcggcaa cggcgggaag atatccctct gctggctggc cattttcttc agcgctttgc
    26461 cgagcgtaat cgcaaggcgg taaaaggctt tacgccacag gtgatggatt tgctgatcca
    26521 ctacgactgg ccgggaaata ttcgtgaact ggaaaacgcg gtggaacggg cggtggtgct
    26581 gctgaccggg gaatatattt ccgaacgcga gctgccgctg gcgattgcca gtacgccgat
    26641 cccgctgggg caaagtcagg atattcagcc gctggtggaa gtggaaaaag aggtgattct
    26701 ggcggcgctg gagaaaacgg gcggcaacaa aaccgaagcc gcccgtcagt tagggatcac
    26761 gcgcaaaacg ctattggcaa aactgtcgcg ttagttctgc tcgcgttcga tggcgcgcca
    26821 accgatatct ttccggcaga agcaatcatc ccagtggata tcggtcatta aggcgtaggc
    26881 gcgtttctgc gcttctgcca cggtatgacc cagcgcggtg acgcacagta cgcgcccgcc
    26941 gctggttacc acctgctcgt catccgccag ttttgtgccc gcgtggaaca ctttgccgtc
    27001 agccacttct tccagcggca ggccgtggat cacatcgccg gtgcggtaat cgcccggata
    27061 tccacccgca gccatcacca cgctgagaga agcacgttca tcccactctg acgttttctc
    27121 gtccagtttg ccttcacagg ccgccaggca gagctcaacc agatcggact tcatgcgcag
    27181 cataatcggc tgggtttccg gatcgccaaa gcggcagtta aattcaataa ccttcggatt
    27241 gccctgtttg tcgatcatca ggcccgcata gagaaaaccg gtgtaggtgt tgccttccgc
    27301 cgccatgcct ttcacggttg gccagatgat acgttccatg gtgcgctgat gaacttcatc
    27361 ggttactacc ggcgcggggg agtaagcgcc catcccgccg gtgtttggtc cgttatcttt
    27421 atcgcctacg cgtttgtgat cctggctggt agccatcggc agcacatgct cgccgtccac
    27481 catcacgata aagctcgctt cttcgccatc gaggaactct tcgataacga tgcgatgacc
    27541 cgcgtcgcca aaagcgttgc ccgccagcat atcgtgaaca gccgcttccg cttcttccag
    27601 cgtcatcgcc acgataacgc ctttcccggc agccagaccg tccgctttaa tgacgattgg
    27661 cgcgcctttc tcacgcagat acaccagcgc aggttctacc tcggtgaagt tctggtattc
    27721 cgccgtaggg atcttatggc gggccaggaa atctttggta aacgcttttg agccttccag
    27781 ttgggccgca cctgcggttg ggccgaagat tttcagcccg gcggcgcgga aggtatcgac
    27841 cacgcctttc accagcggcg cttccgggcc gacgatggtc agatcaatct tttcgttttg
    27901 tgcgaaatcc aacagcgccg ggatatcggt cacgccgata gcaacgtttt gcagcgcggg
    27961 ttccagcgca gtgcctgcat tacccggagc aacaaaaaca gtctcaacca gcggcgactg
    28021 ggccgctttc caggccagcg cgtgctcgcg cccgccgtta ccaatcacta atactttcat
    28081 ctattgctcc attaatggcg gaagtggcgc atgtcggtga agagcatcgc aataccgtgc
    28141 tcgtcggcgg cggcaatcac ttcgtcatca cggatggaac cgccaggctg gattacgcag
    28201 gtcacgcccg cagcggcggc ggcatcaata ccgtcgcgga acgggaagaa cgcgtcagaa
    28261 gccatcgagg aacctttcac ttccaggcct tcatcggcag ctttaatacc ggcgattttc
    28321 gcggagtaca cgcggctcat ctggcccgcg ccaataccga tggtcatatt gtttttggca
    28381 tagacgatag cattggattt cacgaatttc gccactttcc agcagaacag cgcatcacgc
    28441 agttcctgtt cggtcggctg acgttgggtg acgacgcgca gttcttccgc accgaccatc
    28501 cccaggtcac gatcctgaac cagcagaccg ccgttcacgc gtttgaaatc aagacccgga
    28561 acacgctcgc cccactgacc gcaggtcaga acgcgtacgt tctgcttggc ggcggtgatt
    28621 ttcagggctt cttcgctggc ggaaggcgca ataatcactt cgacaaactg acgagaaatg
    28681 atggcctgtg cggtttccgc atccagctcg cggttaaagg caatgatgcc gccgaatgcg
    28741 gaggttgggt cggttttgta cgcgcgatcg taagcatcaa gaatggaatt gccgatagct
    28801 acaccgcaag ggttggcgtg cttcacaatc acacatgccg gctcggcgaa ctctttcacg
    28861 cactccagcg ccgcatcggt atcggcgatg ttgttataag agagggcttt accctgaacc
    28921 tgggttgcgg tagcaacgga ggcttctttc acattctctt ctatatagaa ggcagcctgc
    28981 tggtggctgt tctcgccgta acgcatatcc tgcttcttaa tgaagttcag gttcagcgtg
    29041 cgtgggaagc gaccggcggc ttctttgctt tcaccgtggt acgccggaac catgctgccg
    29101 aagtagttgg caatcatgct gtcgtaggcg gcagtgtgtt cgaaggcttt gatggcgagg
    29161 tcgaaacggg ttgcaagcgt cagcgatcct tcgttgtcat ccatctcttt aataatggcg
    29221 tcgtagtcgc tgctcttcac cacgattgcg acatctttat ggttcttagc ggcggagcgc
    29281 accatggtcg ggccgccgat atcgatgttc tcaaccgcat cttccagcga gcagccttca
    29341 cgagcaacag tctgggcgaa cggatacagg ttaacaacca ccatatcgat aggctggatc
    29401 tgatgttctt ccataatggc atcgtcctgg ccgcgacggc ccagaatgcc accatgtact
    29461 ttcggatgca gggtcttcac gcgtccatcc atcatctccg ggaaaccggt gtaatcggaa
    29521 acttcggtta ccggcagacc tttttctgct aacagacggg cagtgccccc tgtagacagc
    29581 agctccacac cgcgtgcgga aagtgcctgg gcgaattcga cgataccggc tttgtcagaa
    29641 acactgagca gagcgcggcg gactggacga cgttgttgca tggtaaatcc cctgggtttg
    29701 actattacag agagcgttag ctgaattttt cgcgaaaaac tcagctaacg cccctaacgg
    29761 ggcatcctta tttttcgccc gcattgtaac gaaaacgttt gcgcaacgct cgcgaatttt
    29821 tctctttcaa tggtgatcac aattttgaca gtggttaccg tgggaaaaat acagaaatta
    29881 cattgatgat tgtggataac tctgtgcgta aaaaggtata aagcggggtt ttgctgggga
    29941 atgcagcagt cagtcatttt tctgcaattt ttctattgcg gcctgcggag aactccctat
    30001 aatgtgcctt catcgacacg gcggatgtga atcacttcac acaaacagcc ggttcggttg
    30061 aagagaaaaa tcctgaaatt cagggttgac tctgaaagag gaaagcgtaa tatacgccac
    30121 ctcgcgacag tgcgctaaa
//
LOCUS       DADLSA010000035        29653 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.035, whole genome shotgun sequence.
ACCESSION   DADLSA010000035 DADLSA010000000
VERSION     DADLSA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.035"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(93..1064)
                     /gene="secF"
                     /locus_tag="JN691_001593"
     CDS             complement(93..1064)
                     /gene="secF"
                     /locus_tag="JN691_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAY7243066.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFCKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(1075..2922)
                     /gene="secD"
                     /locus_tag="JN691_001594"
     CDS             complement(1075..2922)
                     /gene="secD"
                     /locus_tag="JN691_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAY7243067.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(2950..3282)
                     /gene="yajC"
                     /locus_tag="JN691_001595"
     CDS             complement(2950..3282)
                     /gene="yajC"
                     /locus_tag="JN691_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAY7243068.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(3305..4432)
                     /gene="tgt"
                     /locus_tag="JN691_001596"
     CDS             complement(3305..4432)
                     /gene="tgt"
                     /locus_tag="JN691_001596"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAY7243069.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(4487..5557)
                     /gene="queA"
                     /locus_tag="JN691_001597"
     CDS             complement(4487..5557)
                     /gene="queA"
                     /locus_tag="JN691_001597"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAY7243070.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIGRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            5650..6231
                     /gene="acpH"
                     /locus_tag="JN691_001598"
     CDS             5650..6231
                     /gene="acpH"
                     /locus_tag="JN691_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HAY7243071.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(6236..8053)
                     /gene="malZ"
                     /locus_tag="JN691_001599"
     CDS             complement(6236..8053)
                     /gene="malZ"
                     /locus_tag="JN691_001599"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAY7243072.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGVLLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNAVQWQCKEGHGQLTDRILALPAISATVWMN"
     gene            complement(8209..9582)
                     /gene="proY"
                     /locus_tag="JN691_001600"
     CDS             complement(8209..9582)
                     /gene="proY"
                     /locus_tag="JN691_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAY7243073.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGVGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(9658..10977)
                     /gene="brnQ"
                     /locus_tag="JN691_001601"
     CDS             complement(9658..10977)
                     /gene="brnQ"
                     /locus_tag="JN691_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAY7243074.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(11384..12679)
                     /gene="phoR"
                     /locus_tag="JN691_001602"
     CDS             complement(11384..12679)
                     /gene="phoR"
                     /locus_tag="JN691_001602"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAY7243075.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(12737..13426)
                     /gene="phoB"
                     /locus_tag="JN691_001603"
     CDS             complement(12737..13426)
                     /gene="phoB"
                     /locus_tag="JN691_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAY7243076.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            13616..14818
                     /gene="sbcD"
                     /locus_tag="JN691_001604"
     CDS             13616..14818
                     /gene="sbcD"
                     /locus_tag="JN691_001604"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAY7243077.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            14815..17958
                     /gene="sbcC"
                     /locus_tag="JN691_001605"
     CDS             14815..17958
                     /gene="sbcC"
                     /locus_tag="JN691_001605"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAY7243078.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTTLQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            18084..19268
                     /gene="araJ"
                     /locus_tag="JN691_001606"
     CDS             18084..19268
                     /gene="araJ"
                     /locus_tag="JN691_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAY7243079.1"
                     /translation="MKKVILSLALGTFGLVMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(19411..20319)
                     /gene="mak"
                     /locus_tag="JN691_001607"
     CDS             complement(19411..20319)
                     /gene="mak"
                     /locus_tag="JN691_001607"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAY7243080.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            20444..21355
                     /gene="rdgC"
                     /locus_tag="JN691_001608"
     CDS             20444..21355
                     /gene="rdgC"
                     /locus_tag="JN691_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAY7243081.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(21433..21888)
                     /locus_tag="JN691_001609"
     CDS             complement(21433..21888)
                     /locus_tag="JN691_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243082.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRLIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(21885..22220)
                     /locus_tag="JN691_001610"
                     /pseudo
     CDS             complement(21885..22220)
                     /locus_tag="JN691_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(22375..22659)
                     /gene="ppnP"
                     /locus_tag="JN691_001611"
     CDS             complement(22375..22659)
                     /gene="ppnP"
                     /locus_tag="JN691_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAY7243083.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(22731..23408)
                     /locus_tag="JN691_001612"
     CDS             complement(22731..23408)
                     /locus_tag="JN691_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HAY7243084.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIYAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(23666..23857)
                     /gene="yaiA"
                     /locus_tag="JN691_001613"
     CDS             complement(23666..23857)
                     /gene="yaiA"
                     /locus_tag="JN691_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAY7243085.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRVDHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(23907..24431)
                     /gene="aroL"
                     /locus_tag="JN691_001614"
     CDS             complement(23907..24431)
                     /gene="aroL"
                     /locus_tag="JN691_001614"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAY7243086.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(24614..25072)
                     /locus_tag="JN691_001615"
     CDS             complement(24614..25072)
                     /locus_tag="JN691_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAY7243087.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            25192..26001
                     /gene="proC"
                     /locus_tag="JN691_001616"
     CDS             25192..26001
                     /gene="proC"
                     /locus_tag="JN691_001616"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAY7243088.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(26018..27133)
                     /gene="adrA"
                     /locus_tag="JN691_001617"
                     /gene_synonym="dgcC"
     CDS             complement(26018..27133)
                     /gene="adrA"
                     /locus_tag="JN691_001617"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAY7243089.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(27235..27555)
                     /gene="psiF"
                     /locus_tag="JN691_001618"
                     /pseudo
     CDS             complement(27235..27555)
                     /gene="psiF"
                     /locus_tag="JN691_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
     gene            complement(27674..29089)
                     /gene="phoA"
                     /locus_tag="JN691_001619"
                     /pseudo
     CDS             complement(27674..29089)
                     /gene="phoA"
                     /locus_tag="JN691_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(29190..29450)
                     /gene="iraP"
                     /locus_tag="JN691_001620"
     CDS             complement(29190..29450)
                     /gene="iraP"
                     /locus_tag="JN691_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAY7243090.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGVLYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         7417 a         8003 c         7309 g         6924 t
ORIGIN      
        1 gtttcccaag atgcggggat tttcgcaatc tccatacatc aaaaaaaatc ccgatcttct
       61 gaccgggatt ttcaacatca acgggaactt gattaaggca gaattgacgg ctgatccgcc
      121 ccttcttttt ccactttctg ctgcaacatg tgttcgcgct tcatacccag tttcagagcc
      181 aacgcagatg ctacgtagat ggaagatgca gtgccgatgg aaacaccgat aagcatggtc
      241 agcgagaagc cttccagtac cggaccaccg aagagataca gcatcaggat aaccatcaag
      301 gtagtaccgg atgtgatcaa ggtacggtgc agcgtctggg tcaaggacac gttaaagatt
      361 tcgtaaggcg taccgcgacg gatcttgcag aagttttcac gaattcggtc cgataccacg
      421 atactgtcgt taagcgagta accgataacc gacatcaacg atgccacaat ggtcaggtca
      481 atctcgatat ggaataacga caaaataccc agcgtaatga taacgtcgtg cgccagcgca
      541 ataaccaccc ctgccgccag tcgccactca aagcggaaac ctacgtacac gaggatagac
      601 agcagcgctg ccatcaacgc catcgcaccg gtttgcgcaa ggtctgcccc cacgctcgga
      661 ccgacgaact caatacgctt cactgctgca ttctgattgg tggattcgtt aatcaccttc
      721 agaacttggc tgcccagcac ctgaccgccg gtttcgcctt cagcaggcgg catacggacc
      781 atgatgtcgt ggctgctacc aaagttttgc agcattggct cttcaaaacc ggctttttgc
      841 aatgcatcac gcattacgtc aatttcagcc ggtttttcga gcgtaatttc gataaccgta
      901 ccaccggtga aatccagccc ccagttaaag ccgcgcacgc ccataataac gatagcagcg
      961 attaacagca gaccagagat gccgaaagcc cagtagtccc agcgcataaa gtcatagact
     1021 ttacggccgt ggtttagttg ttcaacagta tattcctgtg ccacatcgca ctcctcagat
     1081 tgacagcttc ttgacgcgct tgccgccata tagcaggttt acgatggcac gcgtaccgac
     1141 aatcgcggta aacatcgacg tcgccacacc gataccggta gtaatcgcga accctttaat
     1201 tgccccggta cccactgcgt acaggatgat gactttaatc agcgtggtga tgttcgcatc
     1261 gaagatagaa ctgaatgcgc cacgataacc ttcatcaatt gcctgttgaa cagtacgtcc
     1321 gttgctcaac tcttctttaa tacgttcgtt gatcagtacg ttcgcatcga ccgctaccgc
     1381 aagggttaag acgatacccg cgatacctgg catactcagt gttgcgcctg gcaacagcga
     1441 cataataccg acgatcaaga tcaagttggc aatcagagca ctggtcgcaa tcagaccaaa
     1501 cttcttatag aagatgatca tgaacagaat agaaaccagc agaccggcaa ggcaagcctc
     1561 cagcccctgc tcaatgttct gcatacccag ggtcgggcca attgtacgtt cttcaacaat
     1621 ctgaatcggc gcgatcaacg caccggcacg cagcagcagt gacagctgac gggcttcgtt
     1681 cgggttgttg atgccggtga tacggaagct gttacccaga cgagactgga tgttggcgat
     1741 gttaatcacc tcttcctgtt tcaccagaac cgcacgacca tttgcatctt tcttaccgct
     1801 gtctttgtac tccacaaaca gggttgccat cggtttgccg atgttgtcct tggtgaagtt
     1861 agacataatg ttgccaccag cgctatcgag cgagatgtta acctgcggct ggttgtattc
     1921 gtcctggctg gaagtggagt cggtgatatg gtcaccagtc agaattacgc gtttgtacag
     1981 cacaaccggc tgaccttcgc gggtctgttt cacttcagag tcgcccggta cgcgaccgga
     2041 tgccgcagcg gcctgatcaa cattggtgtt taccagacgg aattccagcg ttgccgtcgc
     2101 gcccagaatc tctttcgcac gcgcagtgtc ctgaatacca ggcagttcaa caacgatacg
     2161 gtcagcaccc tgacgctgaa ccaccggctc cgccacgcca agttggttta cacgattacg
     2221 caggatatta atgttctgct gcaccgcata ttcacgcgct tcactcagac gagcatcgct
     2281 cattaccgca cgcagctggt tgctgccctg gctgctaatc accaggtccg gatggcgctt
     2341 gctcagatac gcaatggctt catcacgagc tttagcatcg cggaaagtga tgctcaggcc
     2401 gtagttgttt tctttacgaa cagtggtata cgggatgccc ttttcgcgca ggtcactgcg
     2461 taggctatcg atattttgtt cctggagttt gccaagcgcg gtgtccatat ccacttccat
     2521 caggaagtga acgccgccac gcaggtcaag gccgagcttc atcggctcag catgaatagc
     2581 tgccagccag cgcggcgttg ccggggcaag gttaagcgcc acgacgtatt tgtcacccat
     2641 aacgcccatt aatgcttcac gagcgcgcaa ctgggtgtca gtggagtcga agcgcgcaag
     2701 aatagcgccc tcttccagtg ccacagactt agcagttatt ttttcttctt gtaacgtttt
     2761 ctggacctgg atcagcgttt gctcactggc ggcgactccg cgcgcaccag tgatctgaac
     2821 agccggatcc tcaccaaaca ggttgggaag cgcatacagc agaccgatga caatcaccac
     2881 gatcagcatg acgtacttcc acaaaggata acggtttaac acggcaattc ccttagggaa
     2941 aaattttaat tacagcgcct tcatggtgcc tttcggcagg acggcagcta cgaagtcacg
     3001 tttaataact acttcagtgg tgtcattcag cgcgatagca atgtagccgt tttccgctac
     3061 tttggttacg cgaccaacca ggccaccgtt cgtcagaact tcatcacctt tggcaatgga
     3121 gtccatcagc tttttgtgtt ctttggtgcg cttctgctgt ggacgcagga tcatgaaata
     3181 gaaaatcaga ccgaatacca ccagcatcaa aatcaaagac atcgggctac cttgcgccgg
     3241 tgcacccgtt gccgctaccg catcagaaat aaaaaagctc attaaatttc cctcattatt
     3301 aatattaatc aacgttcaaa ggtggtactt ctcgcccctg acgctggtaa aaatcagtta
     3361 cgaagctctc taatttaccc tcttcaatag ccttgcgtaa acccgccatc aaacgctggt
     3421 agtaacgaag gttatgaatg gtgttgagtc gcgcgcctaa tatttcgttg caacggtcga
     3481 gatgatgcaa gtaagcgcgt gaataattgc gacaggtgta gcaatcacac tcaggatcga
     3541 gtgggccagt atcgctctta tatttcgcat tgcggatttt caccacgcca tcggtcacga
     3601 acaaatgacc atttcgggcg ttgcgggttg gcattacgca gtcaaacata tcgataccgc
     3661 gacgtacgcc ttcaaccagg tcttccggtt taccaacgcc catcaggtaa cgcggtttgt
     3721 ctgccggaat ttgcggacac acatgctcca gaatacggtg catatctgct ttcggctcac
     3781 ccacagccag accgccgaca gcgtagccat caaagccgat atctaccaga cctttaacag
     3841 atatatcacg taaatcttcg taaatactgc cctgaattat accaaataac gcatttttgt
     3901 taccaagact gtcaaaacgc tcacggctac gcttcgccca acgcagagac atctccatag
     3961 agcgttttgc gtaatcccag tcagcaggat acggcgtaca ctcatcaaag atcatgacga
     4021 tatccgaacc aagatcgtac tgaatctcca tcgacttttc cgggtcgagg aaaatcgggt
     4081 cgccgttgat cgggttacgg aagtgaacgc cctgttcggt gattttacga atatcaccaa
     4141 ggctgaagac ctggaagccg ccggagtcgg taagaatcgg tcctttccac tgcataaaat
     4201 cgtgcagatc gccgtgcagt ttcatgattt cctggcccgg gcgcagccac aggtggaagg
     4261 tgttgccgag gataatttgc gcgccagtgg cttcaacttc ttccggcgtc atccctttta
     4321 cggtgccgta ggtgccaaca ggcataaaac aaggcgtttc cactacgcca cgatcaaaga
     4381 ccaggcggcc acggcgtgcg cgaccgtcgg tggtgtccag ttcaaatttc attttttctc
     4441 cactacgtca gaaaaacagt ccaacgttta aaccagcgcc gcggaattac tccccgacgc
     4501 gctcattaat tgcctgcgga ttgtacgtga taaacatcgc atcaccgtaa ctaaaaaagc
     4561 gatatttctc ttctaccgcc gctttatagg cattcatggt gtgttgataa ccggcaaagg
     4621 ccgaaaccag cataatcagc gtcgactctg gcaagtggaa gttcgtcacc agcgcatcga
     4681 ccactttgta ctggaagccc ggatagataa agatttgggt atcgtcaaag aacggttcga
     4741 taagatcgtt ttttgccgcc tgtgccgcgc tttccagcga acgtactgaa gtggtgccaa
     4801 ccgcaatcac ccggttaccg cgcgctttcg ccgccagtac cgcgtctacc acatcctgcg
     4861 gtacttcagc gtattccgag tgcatgatgt gatcttcaat ggtgtcgacg cgcaccggct
     4921 ggaaggtgcc cgcaccaacg tgcaacgtca caaacgccat ctccacgcct ttggcgcgta
     4981 atttttccag caaaggctcg tcaaaatgca gaccagcggt cggggctgca accgcgcccg
     5041 gtttttcgct ataaacggtt tgataaagtt cgcggtcagc gtcttcgtcc ggacgaccga
     5101 tatacggcgg cagcggcata tggccgatgc tgttgaggat atccagtacc gagcgttcat
     5161 cattaaattc gacttcaaac agtgcgccgt ggcgcgcggt cattgttgcg ttgatacttt
     5221 cgtcatcgcc cagcaacagt tctgcgccag gttttggcgc tttcgaggcg cgaatatgcg
     5281 caagaatgcg tttgtcgtcg agcatccgct caaccagcac ttcaatcttg ccgccgctgg
     5341 ctttacgccc aaacagacgc gccgggatca cgcgggtatt attaaaaacc agaagatcgc
     5401 cggggttgag cttatcgagt aaatcggtga aagtaccgtg cgtcagcgcg cccgtcggcc
     5461 cgtccagcga cagtaaacga cagctactgc gttcaggcat gggatagtgg gcaatcaggg
     5521 attcgggcaa ttcaaaggag aaatcggtaa cgcgcatgac actgactcag actaaaataa
     5581 gagtcgggta gtctagtgcc ggggcgctct ccctgcaacc tttacccccc tttggataga
     5641 atataatcga tgaatttttt agctcacctg catttagccc atctcgcgga aagctcgctt
     5701 tctggcaatt tactggctga tttcgtacgc ggaaatcctg aagaaagttt tccgcccgac
     5761 gtcgtggctg gcattcatat gcatcgacgt atcgacgtat tgactgacaa tctgccggaa
     5821 gtccgcgaag cacgggagtg gtttcgtagt gaaacgcgcc gcgttgcgcc tattacgctg
     5881 gatgtcatgt gggatcactt tctttcccgc cactggtcgc agctgtcgcc ggactttccg
     5941 ctacaggaat ttgtctgtta tgcccgcgag caagtgatga cgattttgcc ggactcaccg
     6001 ccacgtttta tcaatctgaa caattacttg tggtcagagc agtggctggt gcgctatcgc
     6061 gatatggatt tcatccagaa cgtgttaaac ggcatggcaa gccgccgccc acgtctggat
     6121 gccctgcgtg actcctggta cgatttagac gctcattatg acgccctcga aacccgcttc
     6181 tggcagtttt atccgcggat gatggcgcag gcgtcacaca aggcgttata aaacgttagt
     6241 tcatccatac cgtagccgaa atggcaggca aagccagaat cctgtcagtc agttgcccat
     6301 gcccttcttt gcattgccat tgcacggcat tgagaaacgg tgacgcgggt agcaccactt
     6361 cacaggcctc gccacggttg attgccacca gtacacgttg ctgattcagc acgcggacaa
     6421 ataccactac gttatcttcc gcatacagca cctgacagcc gccacgacgc agcgcctgac
     6481 ttttcttacg cagcacaatc attcgccggt acagcgcgaa taacgccgta tcctgctttt
     6541 ccacctgcca ggggaacggt ttacggcaaa acggatcgtt tttaccatcc agtcccactt
     6601 catcaccgta ataaatgcac ggtacaccag gccaggtgaa cagccagacc accgccagcg
     6661 gcaggcgcgc aatatcccga ccgagcagcg ttttaaatcg cgcagtatcg tggctatcga
     6721 gctgattaaa catacgtaat tgttgttgat gagaaagccc tgcgcggtaa ttatccatcc
     6781 aggccataca ggtttgggcg tcaatttgct gcggatcata agagatgtcg gtattggcaa
     6841 gaaatcccca caacgggaat gtgaagccac gatagttcat ggcagcatct tccacatcgg
     6901 cctgtaacca ttgccgtgca tcgccaaaat gttcgccgac aatatacgcg tccggctggg
     6961 tttctttcgc cgcttcggtg atcccggcaa cgtgctgcat attattgcgc gccccacccg
     7021 cctcccccag catatgcacc acatccagcc gccagccgtc catattccac ggcgctttca
     7081 gccagtggcg gacaatactg tcttccccgc gataaatttc attcaccaga ctttccgact
     7141 gataatccag cttcggcaag ctggcatagc caagccagtc gagcgccgtg ccatcatcac
     7201 taaacgagta ccagtcgcgc cagggcgatt cagagttgtg acaagctccc cccgtgccaa
     7261 gattgtgcct gtcaaaccag gcatgggaat cgccactgtg gttaaacacg ccgtccagca
     7321 ccagccgcat tcccagctgc tgcgtattgt gtcgcaaacg cagcaacacc ccatcaccgc
     7381 caaactgcgg atcgacatgg cgataatcct cggtatcgta tttatgtacg ctgggagctt
     7441 taaacaccgg attgagatac agcgctgtca cgccaagctt tttcagatac ggcagttttt
     7501 cgcttatccc gtccagatcg ccgccataga acgttgatcc gcccgcctgc gccgtgaccg
     7561 gttcatccca gtcacgcaag atgatctctt gtccggctgc atgatggtaa tagacatgat
     7621 cctgttcagc ttcacgagga agactacgcg caaaacgatc agggaagatc tgataaaaaa
     7681 tctgatccgc agcccattgt gggccgatat ccggtacatc gacggcaaac tgctccagtc
     7741 gtgccggcgg cattcggctg aagccctgcg gtgtaaacca acgctggcga tcgtgccaca
     7801 gcagtttgaa actgtaacgc cgccggggtt gtccgctgga gagatcaatc gccgcacgcc
     7861 atgcggtgac gcctggctgc ggctgactgc gctgcttatg cattggtact gacatttctt
     7921 cgttatcgtg ttctgtacgc agcataatgc gctgcggtgg gtcttcgccc gtcagccaca
     7981 gtgtaatgag cagttgatct ttgctttgtt taacaaatgg gggcaccggc aggtgccatg
     8041 catttaacat cataattccc ctgtgatgaa aatctgatca ccttgccata gcctaatgca
     8101 ggatcactca tcatctgaag gatatttggg ggaggaggac gggggcggat aactttcgga
     8161 atagccggat caggatttcc tcctgaaccg gcttacagaa cgcaagtatt attggttttc
     8221 agccagctga cgatcgtggc ggcgtttaaa catccagcca atcaacagca caacaatcca
     8281 cgcgaaaccg acatacagcg agatacgcgt atccgggtga taaccaatca acccgataat
     8341 aaagagcagg aaaatcagcc cgccgatggt cgttgctacc ccacccggca ctttaaattt
     8401 cagcgcctta acttcttctg gcggcaaacg gcgacggaag gcgatttgcg acagcaggat
     8461 cataatccac acccataccg tggcgaaggt tgccagcgaa gcgatcacca ggaagacgtt
     8521 ttccggcatg atgtagttca gatacaccgc aaacagcagc gcggtagtca tcaccagcac
     8581 cgtaacccac ggaataccgc gacgcgacgt tttgctgaaa attttcggtg cgctgccctg
     8641 ctctgccata ccgtggagca tacggcctac gccaaatacg tcactgttaa ttgccgacag
     8701 cgaagcggtc agcacaacaa agttaagaat gctggcggca aaggtaatgc ccatatgctg
     8761 gaacgtcagc acgaacgggc taccggcagt gccaacctga ttccacgggt agatagacat
     8821 aatgacgaac agcgtaccga cgtagaacac cagaatacgc atcggcacgg agttaatcgc
     8881 acgcggaatc gatttctcag gatctttcgc ttcaccggcg gtaatcccga taatttcgat
     8941 cccaccgtaa gcaaacatca ccatttgcaa cgacattacc atgccaagcc agccgttact
     9001 gaagaagccg ccgttgctcc acaggttatg aataccggtc ggttgcccgc cgttgccaac
     9061 cccccagatg atgatgccga aaccggcgac aatcatgatg atgatggtgg cgactttaaa
     9121 gaacgagaac cagaattcca gctcaccgaa gaccttcacg ctcatcaggt ttacggcgca
     9181 aatgatcagc accacgctca gtacccaaat ccagtgcggc accgtcggga acaagacacc
     9241 catatagata ccaaaagcgg tcacatcggc gatggcgaca ataaggattt caaagcagta
     9301 ggtccatccg gtaatgtaac ctgccagcgg gccaaggttt tcctgcgcat aacgcgagaa
     9361 agagctggcg gccgggttat gtaccgacat ttcccccagc gcacgcataa tgatatacgc
     9421 cgcgatacca ccgataatat aggccaacaa cacgctcgga ccggccattt tgatggcgtc
     9481 tgccgaaccg taaaacagcc cggtgccaat tgctgaaccc agtgccataa agcgtatgtg
     9541 gcgggtactt agcccacgct ttagcttatt cttactttcc atcaattcct aacccatcaa
     9601 cacaataaaa aaccacgggg catcgcagcc ccgtggttaa aacaaatgtt cagtgattta
     9661 gtgagcgctg gaggtcacct gacgacctgc cgcgcgatcc cagataatgg ccagaaccac
     9721 catcaccact gttggcatta accacgccag accttgttcg gccagcggta aacgctgcgc
     9781 ccaggacggt aagatatcgc tgaatgcaga tgctttgatc ccgtcgagaa taccaaaaag
     9841 caggctgata aacatcggcg gagcaatcac gcgggacgaa ttatgccacc atgagcgtgt
     9901 aaaacttaat acaaccagtg cgatacacgg cggataaata gcggtcagca ccggtacgga
     9961 gatctggatc agctggctta agccgaggtt agacaccacc atcgagaagc cgccgaggat
    10021 aaacaccagc gtacgataag agagcggtac gtactgggca aagaattctg cacaagcaca
    10081 ggtcaggcca accgccgtga ccagacaggc gatgaagatt aacgccgcca gcaggaagct
    10141 accgccgccg ccaaaggtgt gctgaacgta agcatgcaga atagcagcgc cgtttgcaga
    10201 ctgatcgacc agcgacgcgc tgtctgaacc cagacagaac agcgccaggt acagcaaagt
    10261 cagaccaaca cccgccatca ggccagccca gacggtataa cgggtcagca gacgcgcttc
    10321 ggtaacgcca cgagaacgcg ccgcgttaac aataacgata ccaaacacca ttgcgcccag
    10381 cgtatccatg gtcagatagc cgttaacgaa gccgttagaa aacgcagcgt tttgataagc
    10441 ctcagtcgcc gtgctgatag aacccgccgg ccagacaatc gcggcaacag acaggatgac
    10501 cagcgcgata attttcagcg gcgcaaggaa gttgcccacg gtatccagca gcttgcccgg
    10561 atagagcgaa accagaataa cgatagcgaa atagaccagg ctgtagataa acagcggcag
    10621 cgcggaatca cccgtcagcg gcgcaatccc cacttcaaag gaaacggtag ctgtacgcgg
    10681 cgtagcgaaa agcggcccca ccgccaggta acaaaccgtt gccagcagta cgccagcgac
    10741 tttaccgatt ggcgtgctga ggctgtcaac accgccgcca acttttgcca gcgccactac
    10801 cgttaatacc ggcaggccaa cggcagtaat gaggaagccg aatgccgcag tccagacgtg
    10861 ttcgcctgcc tgtaagccga ccattggagg gaaaataatg ttacctgcgc cgacgaacaa
    10921 cgcaaatgtc ataaagccca gagcgatgat atcgcgcgat cttaattgat gggtcataaa
    10981 tcatactgcc tgtggatgtg gtgttgtaac gttaaatttt tgtgccaatc cctggggatg
    11041 atacagcggt atatttaaac aattttagcg ggaaatactc ccaaccaggc gcggcgaaga
    11101 atagtttttc gatttaccgc gttaatgcag tcagtatgac agcacctgaa gcgcaattta
    11161 aacgcttata acgttttaaa gcaaggaggg atctaaaaac cagaggtata tagcgataag
    11221 aaatcaccaa ccagtataaa acactatttc cgtgaaaaag acatggctaa tcatgcgaac
    11281 aaaaaatcac caaccgttgt aaaatctaac gcccgtattt tgactgcaaa gaaaataagc
    11341 cagcatcggc tggcttatgg caataaaaga tgacaaaggc ggattaatcg ctgtttttgg
    11401 caattaaacg ttccgggata acaaaactga aacgtgttcc ttttcctact gtactctcaa
    11461 tattcaggcg actttcgtga tgattcaccg catgtttcac gatcgctaac cctaatccgc
    11521 taccgccggt ttgccgggaa cgcgctttat caacgcgata aaaacgctcg gtcaggcgcg
    11581 gaatatgctc cggtgcaatg cccggtccgt tatcttcaac gctaaattcg gcaccgtgcg
    11641 gcactcgctg ccagcgtacg gtgatatgcg tgccttccgg cgtatgattc acggcgttat
    11701 agaccaggtt cgaaatcgca ctgcgtagct gatcttcgtt gccagacacc ttgaggccgt
    11761 tatctatctc aaaggtaaat gtctgttttt tctgactcag agtctgagcc tcgcgctcaa
    11821 caacgcgcag catcatcggc acatcaacct tttcattgag caattgcgtc ggcgcggctt
    11881 caattttcga cagcgttagc aactgcttca ccagcccttc catccgctgg gtctgctcgc
    11941 gcatggtgtg caacgctttt tcgcgtaccg cgccttccag cggctgctca tccatcatct
    12001 ccaggtaacc ctgtaacacg gtcaatggcg tacgtaactc atggctcacg ttggcaaaaa
    12061 agttacgccg cgccccttcc agttgatgca tttgcgtgac atcacgcgcc accatcagca
    12121 actgtttgtg ggtataaggc atgacgcgaa tttccagatg ccgcccggtg ttgagcacca
    12181 gattgagcgg gcgagaaaaa tcacgcgttt tcagatattg cgtaaactcc gggtaacgca
    12241 gtaggttaag gatgttctgc ccgttatctt ccggccagcg caaaccaaga atttgttgcg
    12301 ccagaccgtt acaccagaaa ataccgccct cttccgtggt cagcaccacc gcgtcgggca
    12361 gcgactccgc gccgctacga aagcgtttaa tcagattgcc cagttcacgg cggcgttttt
    12421 tatttcgcag ctgcatctgg tgtaagccgt atagtagcgg ttcccagcta ccacgccccg
    12481 gtggcggggt catactgcga tccacccaca gccaccatga aaggcgcaat aaattccaga
    12541 aatgccagat aagcagtcct gttaccgatg ccagcaaaaa ccagggcagg taaccaaaaa
    12601 atgcacccag gatgaaagcc gggaggcagc aaagtagcag ctccagcacc agccttttcc
    12661 acgacagccg ttccagcacg taagatactc cagttaagaa atcataagcc ctgctctgcg
    12721 tccgatgagc aaggcgttaa aagcgggttg aaaaacgata tcctgtaccg cgcacggtct
    12781 gcaccatgcg gtcatgcccg ccgggctcca gtgctttacg caggcgacga atgtggacat
    12841 cgaccgtgcg gtcttccaca tacacgttag ttccccagac gtggtttaac agctgctcgc
    12901 ggctgtacac gcgctcagga tgcgtcataa aaaagtgcag cagtttaaat tctgtcggcc
    12961 ccatctccag cggctcttcg cccgccatca ctcggtgaga tgtcgggtcg agacttaatc
    13021 cctgcatctc aatcacctct tccaccgcca ttggcgaaat acggcgcatt accgctttga
    13081 ttcgcgccac cagctccttc ggcgaaaatg gcttggtgat atagtcatcc gcgccggttt
    13141 caaggccgcg cacgcgatct tcttcttccc ctctggcggt caacatcacc actggaatat
    13201 cccgggtcat cgactcgcgc ttgaggtgtt tgatgaactg gataccggag ccgccaggta
    13261 acatccagtc aaggagaatt aaatccggcc agggttcatt cagttgattc acagcactgt
    13321 cataatcttc cgcttcgacc ggctgaaagc cattttgttc gagcacgaag cagaccattt
    13381 cgcgaattgg agcttcatct tctacgacca gaatacgtct cgccatgatt tgccctgttg
    13441 taataaatag gttgcgatca ttaatgcgac gtcattatgc gtcagattta tgacagattt
    13501 atgaaaagct cgtcgcacat atcttcaggt tattgatttc cgtggcgcag aaaaaagcaa
    13561 atggcacatc tgtttgggta taatcgcgac catgcttttt cgccagggaa ccgttatgcg
    13621 catccttcac acctcagact ggcatctcgg ccagaacttc tacagtaaaa gccgcgaagc
    13681 tgaacatcag gcttttcttg actggctgct ggagacagca caaacccatc aggtggatgc
    13741 gattattgtt gctggtgatg ttttcgatac cggctcgccg cccagttacg cccgcacgtt
    13801 atacaaccgt tttgttgtca atttacagca aactggctgt catctggtgg tactggcagg
    13861 aaaccatgac tcggtcgcca cgctgaatga atcgcgcgat atcatggcgt tcctcaatac
    13921 aaccgtggtc gccagcgccg gacatgcgcc gcaaatcttg cctcgtcgcg acgggacgcc
    13981 aggcgcagtg ctgtgcccca ttccgttttt acgcccgcgt gacattattt ccagccaggc
    14041 ggggcttaac ggtattgaaa aacagcagca tttactggca gcgattaccg attattacca
    14101 acaacactat gccgatgcct gcaaactgcg cggcgatcag cctctgccca tcatcgccac
    14161 gggacattta acgaccgtgg gtgccagtaa aagtgacgcc gtgcgtgaca tttatattgg
    14221 cacgctggac gcgtttccgg cacaaaactt tccaccagcc gactacatcg cgctcgggca
    14281 tattcaccgc gcacagatta ttggcggcat ggaacatgtt cgctattgcg gttcccccat
    14341 tccactgagt tttgatgaat gcggtaagag taaatatgtc catctggtga cattttcaaa
    14401 cggcaaatta gagagcgtgg aaaacctgaa cgtaccggta actcaaccca tggcggtgct
    14461 gaaaggcgat ctggcgtcga ttaccgcaca gctggaacag tggcgcgatg tatcgcagga
    14521 gccacctgtc tggctggata tcgaaatcac tactgatgag tatctgcatg atattcagcg
    14581 caaaatccag gcattaaccg aatcattgcc tgtcgaagta ttgctggtac gtcggagtcg
    14641 tgaacagcgc gagcgtgtgt tagccagcca acagcgtgaa accctcagcg aactcagcgt
    14701 cgaagaggtg ttcaatcgcc gtctggcact ggaagaactg gatgaatcgc agcagcaacg
    14761 tctgcagcat cttttcacca cgacgttgca taccctcgcc ggagaacacg aagcatgaaa
    14821 attctcagcc tgcgcctgaa aaacctgaac tcattaaaag gcgaatggaa gattgatttc
    14881 acccgcgagc cgttcgccag caacgggctg tttgccatta ccggcccaac cggtgcgggg
    14941 aaaaccaccc tgctggacgc catttgtctg gcgctgtatc acgaaactcc gcgtctctct
    15001 aacgtttcac aatcgcaaaa tgatctcatg acccgcgata ccgctgaatg tctggcggag
    15061 gtggagtttg aagtgaaagg tgaagcgtat cgcgcgttct ggagccagaa tcgggcgcgt
    15121 aaccagccgg acggtaattt gcaggtgcct cgcgtagagc tggcgcgctg cgccgacggc
    15181 aaaattctcg ccgacaaagt gaaagataag ctggaactga cagcgacgtt aaccgggctg
    15241 gattacgggc gcttcacccg ttcgatgctg ctttcgcagg ggcaatttgc tgccttcctg
    15301 aatgccaaac ccaaagaacg cgcggaattg ctcgaggagt taaccggcac tgaaatctac
    15361 gggcaaatct cggcgatggt ttttgagcag cacaaatcgg cccgcactga gctggagaag
    15421 ctgcaagcgc aggccagcgg cgtcacgttg ctcacgccgg aacaagtgca atcgctgaca
    15481 gcgagtttgc aggtacttac tgacgaagaa aaacagttaa ttaccgcgca gcagcaagaa
    15541 caacaatcgc taaactggtt aacgcgtcag gacgaattgc agcaagaagc cagccgccgt
    15601 cagcaggcct tgcaacaggc gttagccgaa gaagaaaaag cgcaacctca actggcggcg
    15661 cttagtctgg cacaaccggc acgaaatctt cgtccacact gggaacgcat cgcagaacac
    15721 agcgcggcgc tggcgcatat tcgccagcag attgaagaag taaatactcg cttacagagc
    15781 acaatggcgc ttcgcgcgag cattcgccac cacgcggcga agcagtcagc agaattacag
    15841 cagcagcaac aaagcctgaa tacctggtta caggaacacg accgcttccg tcagtggaac
    15901 aacgaactgg cgggttggcg tgcgcagttc tcccaacaaa ccagcgatcg cgagcatctg
    15961 cggcaatggc agcaacagtt aacccatgct gagcaaaaac ttaatgcgct tgcggcgatc
    16021 acgttgacgt taaccgccga tgaagttgct accgccctgg cgcaacatgc cgagcaacgc
    16081 ccactgcgtc agcgcctggt cgcgctgcat gggcagattg ttccccaaca aaaacgtctg
    16141 gcgcagttaa tggtcactat ccagaatgtc actctggaac agacgcaacg taatgtcgca
    16201 cttaacgaaa tgcgccagcg ttataaagaa aagacgcagc aacttgccga tgtgaaaacc
    16261 atttgcgagc aggaagcgcg catcaaaacg ctggaagccc agcgtgcaca gttacaggcg
    16321 ggtcagcctt gtccactttg tggttccacc agccacccgg cggtcgaggc gtatcaggcg
    16381 ctggagcctg gtgttaatca gtctcgatta ctggcgctgg aaaacgaagt taaaaagctc
    16441 ggcgaagaag gtgcggcgct gcgcgggcaa ctggatgcat taacgaagca gcttcagcgc
    16501 gatgaaaacg aagcgcaaag cctccgacaa gatgagcaag cacttactca acaatggcaa
    16561 gccgtcacgg ccagcctcaa tatcaccttg cagccacagg acgatattca accgtggctg
    16621 gatgcacaag atgagcacga acgccagctg cggttactca gccaacgaca tgaattacaa
    16681 gggcagattg ccgcgcataa tcagcaaatt atccagtatc aacagcaaat tgaacaacgt
    16741 cagcagcagc tcttaacggc attggcgggt tatgcactga cattgccaca ggaagatgaa
    16801 gaagagagct ggttggcgac acgtcagcaa gaagcgcaga gctggcagca acgccagaac
    16861 gaattaacca cgctgcaaaa ccgtattcag cagctgacgc cgattctgga aacgttgccg
    16921 caaagtgatg atctcccgca cagcgaagaa actgtggcgc tggataactg gcggcaggtt
    16981 catgaacaat gtctcgcatt acacagccag cagcagacgt tacagcaaca ggatgttctg
    17041 gcggcgcaaa gtctgcaaaa agcccaagcg cagtttgaca ccgcgctaca ggccagcgtc
    17101 tttgacgatc agcaggcgtt ccttgcggcg ctaatggatg aacaaacact aacgcagctg
    17161 gaacagctca agcagaatct ggaaaaccag cgccgtcagg cgcaaacgct ggtcactcag
    17221 acagcagaaa cgctggcaca gcatcaacaa caccgacctg acgggttggc tctcactgtg
    17281 acggtggagc agattcagca agagttagcg caaactcacc aaaagttgcg tgaaaacacc
    17341 acgagtcaag gcgagattcg ccagcagctg aagcaggatg cagataaccg tcagcaacaa
    17401 caaaccttaa tgcagcaaat tgctcaaatg acgcagcagg ttgaggactg gggatatctg
    17461 aattcgctaa taggttccaa agagggcgat aaattccgca agtttgccca ggggctgacg
    17521 ctggataatt tagtccatct cgctaatcag caacttaccc ggctgcacgg gcgctatctg
    17581 ttacagcgca aagccagcga ggcgctggaa gtcgaggttg ttgatacctg gcaggcagat
    17641 gcggtacgcg atacccgtac cctttccggc ggcgaaagtt tcctcgttag tctggcgctg
    17701 gcgctggcgc tttcggatct ggtcagccat aaaacacgta ttgactcgct gttccttgat
    17761 gaaggttttg gcacgctgga tagcgaaacg ctggataccg cccttgatgc gctggatgcc
    17821 ctgaacgcca gtggcaaaac catcggtgtg attagccacg tagaagcgat gaaagagcgt
    17881 attccggtgc agatcaaagt gaaaaagatc aacggcctgg gctacagcaa actggaaagt
    17941 acgtttgcag tgaaataact attcagcagg ataatgaata cagaggggcg aattatctct
    18001 tagccttgct ggtcgttatc ctgcaagcta tcactttatt ggctacggtg attggtagcc
    18061 gttctggtgg ttgtgatggt ggtatgaaaa aagtcatttt atctttggct ctgggcacgt
    18121 ttggtttggt gatggccgaa tttggcatta tgggcgtgct cacggagctg gcgcataacg
    18181 taggaatttc gattcctgct gccgggcata tgatctcgta ttatgcactg ggggtggtgg
    18241 tcggtgcgcc aatcatcgca ctcttttcca gccgctactc actcaaacat atcttgttgt
    18301 ttctggtggc gttgtgcgtc attggcaacg ccatgttcac gctctcttcg tcttacctga
    18361 tgctcgccat tggtcggctg gtatccggct ttccgcatgg cgcatttttt ggcgtcggag
    18421 cgatcgtgtt atcaaaaatt atcaaacccg gaaaagtcac cgccgccgtg gcggggatgg
    18481 tttccgggat gacagtcgcc aatttgctgg gcattccgct gggaacgtat ttaagtcagg
    18541 aatttagctg gcgttacacc tttttattga tcgctgtttt taatattgcg gtgatggcat
    18601 cggtctattt ttgggtgcca gatattcgcg acgaggcgaa aggaaatctg cgcgaacaat
    18661 ttcacttttt gcgcagcccg gccccgtggt taattttcgc cgccacgatg tttggcaacg
    18721 caggtgtgtt tgcctggttc agctacgtaa agccatacat gatgtttatt tccggttttt
    18781 cggaaacggc gatgaccttt attatgatgt tagttgggct agggatggtg ctgggaaata
    18841 tgctaagtgg caggatttca ggacgttatt caccactgcg cattgcagca gtgactgact
    18901 ttatcattgt actggcactg ctgatgctct ttttctgcgg cggcatgaaa acaacgtcgc
    18961 ttatttttgc ttttatttgt tgcgcgggat tatttgccct ttcagcacct ctgcaaatat
    19021 tgttactgca aaatgccaaa ggcggagagt tattaggtgc cgcaggtggg caaatagcgt
    19081 ttaacctcgg tagcgccgtc ggcgcatatt gcggaggtat gatgctgacg ctggggctgg
    19141 catataatta cgtggcgctg cctgccgccc tgctttcgtt tgctgcgatg tcatcgttgc
    19201 tgctgtatgg tcgctataag cgccagcaag cggcggatag tccggtgctg gcgaaaccac
    19261 tggggtagat tactgtcacg gtgcgcgttg cttattgccg gatgcggcgt gaacgcctta
    19321 tccgccctac gcggttctgg cacattttgt aggcctgata agacgcggca agcgtcgcat
    19381 caggcatgat gcgccaattg cctacgtttt ttactcttgt ggccataacc acgcagcgcc
    19441 gcgtacgccg ctggaatcac cgtgcttcgc cttacgcacc ggcgtttcac attcgccgcc
    19501 gaagacaaat tgtttaatca actgcccaac cgtttgatat aaacggtcta cattgctcat
    19561 cccgcccccc aggacaatca catccggatc gagaatattc acgacatgtg ccagcgattt
    19621 tgccagccgc agctcgtagc gacgcaatgc cagttccgct accggatcgc tttcttcaac
    19681 caggcggata atttcactgc ctttcagcgc atgtccgctc aaacgacgat aatccgtcgc
    19741 gaatcccgtg cccgaaataa aggtttcaat acaaccttgt ttaccgcaat aacaagggac
    19801 ttcctcgcga taacgcagtt cgtcttcgtc catccacggt agcggattgt gtccccactc
    19861 acctgccgtg ccattgccgc cgatatgcgt ccgcccattg aatgccacgc ccgcgccgca
    19921 tcccgtgccg ataatcacgg caaataccgt ctgcgctccc gctgccgcgc catctactgc
    19981 ttctgaaacc gccagacagt tggcatcatt tgccagccgc acttcccgct gcaacctcgc
    20041 gcttaagtct ttatcgaatg gctgaccgtt gagccaggtt gaattggcat tcttcaccac
    20101 accggtgtag ggcgaaatcg aacctggaat gcccatacct accgttccgc gctgccctgt
    20161 cgcctgctcc gccatatcaa ccaacgtggc gatcgtttca atagtctgcc ggtaatcatc
    20221 acgcggcgtg ggcagacgat ggcggtacaa ctgctcccct gcatcgccca gtgcaatcac
    20281 ttcagttttg gtgccgccta aatcgatacc tatacgcact gtactctcct tatttttttc
    20341 aatatcaata gcgtagagac ggacaaccgg attggcaatg caaggccgcc gacaattcgt
    20401 tatcatgccc gctaaattta acgacaaggc cgtggaaatt atcatgctgt ggttcaaaaa
    20461 tttaatggtt taccgtctta gccgcgagat ttcgctgcgt gcagaagaga tggaaaaaca
    20521 gctagcctcg atggcattta ccccatgcgg cagccaggac atggcgaaga tgggctgggt
    20581 tcctccgatg ggatcgcaca gcgatgcgtt aacgcacgtt gccaatggtc aaattgttat
    20641 ctgcgcgcgc aaagaagaaa aaatcctccc gtctccggtg attaaacagg cgctggaagc
    20701 gaaaatcgcc aaactggaag cggaacaggc gcgtaagctg aagaaaaccg aaaaagattc
    20761 gctgaaagac gaagtgctgc actctctgct gccgcgtgct ttcagccgtt ttagccagac
    20821 aatgatgtgg ctcgacacgg ttaacggttt gattatggtg gactgcgcca gtgccaaaaa
    20881 agcggaagat acgctggcat tactgcgtaa aagcctgggg tcgttaccgg ttgtaccatt
    20941 gagcatggaa aacccgattg aactgacgct gaccgaatgg gttcgctccg gtagtgcggc
    21001 acagggcttc cagctgcttg atgaagccga gctgaaatcg ttgctggaag atggcggcgt
    21061 gatccgcgcg aagaaacaag atctgaccag cgaagagatc accaatcaca ttgaagccgg
    21121 aaaagtggtg actaaactgg cgctcgactg gcagcagcgc attcagtttg tgatgtgcga
    21181 cgatggttcg ctcaagcgtc ttaagttctg cgacgagctg cgcgatcaaa acgaagatat
    21241 cgaccgtgaa gatttcgccc agcgttttga tgccgatttc atcctgatga ctggtgaact
    21301 ggcagcgtta attcaaaacc tgattgaagg attaggtggc gaagcacaac gttaattgct
    21361 gattttcctt taatgccgga tgcgacgcct gccgcgtctt atccggcgta cgaagccaca
    21421 ccaggcatat aattattcgc tacggcgagc aataattttt agcgcagcaa tattatgcgt
    21481 tttacgccgt aactgatcta atcgcctcat ccctattagc ctgtcagcag cacgcagaat
    21541 attcactaac ccccatttat cgctggtaaa ttgttttgtt aacagctccg cttttggggt
    21601 atcaatatat tcacgcaata aatcagaaat tgaacaaata tccgtcaggt aaggatacca
    21661 gcaagtttca ccgtgaaaat tacgcactcc agcgttacct gcaataaagt ctttcgggta
    21721 atcccagatc acgtttttat ccaacgtgat ccaataacgc ggtaaatcgg tacttccacc
    21781 attttggctg cgcatgggat aacgggtaca gtgaatttgc aggttgatcg ttggcgtcag
    21841 taagtcatag atctcccgct gtaacttgct ccatggacgt tgtgtcattg tttttcctca
    21901 agccagtgac gcgcttcact gcgaattttc cgcgttgccc cggttttcac taaatcacgt
    21961 aactgcgaaa gatcgggttg ttcgacaaat aagagtaatg atggttcctc ttttaaccac
    22021 gccgttttca catcggcagc cagctgtgga ttattaatag ccagcgaccg atcctgaggc
    22081 tcagtcaatg tcgttaacaa cgacgccggg gtatgtgcgt tgcgtaatgc cgtttggcgc
    22141 aaatgatcat tgcgactttg cagcaatgca cgccaccatt catcatctga tttatctttt
    22201 atcgtagcca gttccgcaaa aaatacctgg agatcttcac tgatatttat atcaaccttg
    22261 atgaattatg aatatatcgg aaattgaaaa aatcgcagca aaggaaatat tattatgggt
    22321 taaagagagg atatttcaga aaaatgccct cccgttcaag gggaaggcga ggaattacag
    22381 atagcggcac agataagagg tgggttcggc aacttgaaga tgaaactcac tgtgaccggg
    22441 aacattaaac accgaaccgg cttcatacac ttgccagtcg gtcgcgtcag gcagtaacac
    22501 attcagcgcg ccactgatta ccgtcatctc ttccggctca gcggtgctga aggtgtattc
    22561 gccttcaacc ataacaccca cgctggcgcg accagtgctg ctgctggaaa agccgatcga
    22621 tttcactttg ccggaaaagt actcattact ttgaagcata aactggccct gtctggtagt
    22681 tgaacatcta cttcactata ggggccagag gcgctggctg tcacgcaaaa ttacaccagt
    22741 aattccgcag ccagccgtgc aatcaatacg ttagagagca agacaggaac atcgagctgt
    22801 ttttgcagta aatcacgatg acgttggtga aatcctaaac aatccagcat gatgacatca
    22861 gccccttttg ccagtaattc tttcccagca taaatgattt tttgttctga atcatgaatg
    22921 gggttaccca atgaaaatac cggcgatttc tgcaaaattt gccatttttg cgcctgaacg
    22981 ggcagcatct cctcaaccgg aacgataacc cccacctgat gatcttcaac aatagaggaa
    23041 accagtggag gcaatattcg cgacggctca agaaagatcg tattacgcgc agtcatacta
    23101 ctaatgtttg ctgtactcat taataaaatg acgtcataac cctgattatc gagcacttca
    23161 accacacctt gcagatctcg ctccacttta cgacgcgaaa catgggccag ctggttgtca
    23221 tttaataatg tgagaatggt gtcttcgcct gcttctggcg cgtactccgc catcacttct
    23281 tcacgactta acttccccag caggctatga tgggaaatat tatcttcgtc aatgtattcc
    23341 gtcaggagcg gcaaaacctc ctgcataggt acaatgccga tggtcaggat cgccaacgac
    23401 gcactcattg ttcatccacc ttacttttct ttcgtcgtta attaccgggc aagtgtgaag
    23461 caccactgct gacacttact tgtgctacaa atgacaaaaa gcgtagcagc agacgtgcac
    23521 ggcataatgt aaagattcca aatgattcca gtaatggaat gttattgttt aatattctaa
    23581 ttattagaaa aacatgaatt atgaaaaatg tgacgcagat cccttgtagt ggcactcaga
    23641 atcccttccg agcagtctga tgcggtcact ctttgtcagg gtcctcatag cgttttttcg
    23701 catccatcgc ttcctgagcc gtcggatgtt cactgatcaa cgagtctggt ttaggatgat
    23761 cgactctgag ttggtaccag gttaccgtct gtcctggctt tcctttttca atcgtcacta
    23821 tatatgcttc acgaggataa ggcggtttcg ttggcatcgt tcttccttat ttcacgggat
    23881 gaacgttaag tataggcgct ctaaaatcaa caattgatcg tctgtgccag ggcgctacga
    23941 atttcagaaa tcacctggct gggttcgttt gttgcgtcga tgataatatg cgcaacttcg
    24001 cgatatagcg catcgcgttc ttccagcact tcctgaactt cttcgctcag cggttttccc
    24061 gttaaggttg gccgtaaatc ttcttccggt gcagcttgca gtcggttaac caggactgat
    24121 actggcgcac acaaataaac cacgatcccg ttattttgca tgaagtgacg attaaattcc
    24181 gtcagaataa tgccgccgcc tgtagcgata acggtggatg gcgcagttac cgcttccagc
    24241 gccgccgttt ctctggcgcg aaatcccgcc cactcttccc tttcgacgat ctccgcgacc
    24301 gtcatattga gctgtgattg caaccactga tcggtatcga caaaccgacg gttaagcgaa
    24361 tcggcaaggg ccattccgac cgttgtttta ccacagcccc gaggcccgat cagaaaaaga
    24421 ggttgtgtca tcgtgggttt tccccaatag gtcgcaatgc ggcgaaagcc ggtgtcatga
    24481 gaatagcgat cataccatca aactagtaca atttcgattg taaagaaaaa attccactta
    24541 aagtgaaaat ctcaatacat cccttactat accaataaat attcaagaat gaagtgtaaa
    24601 taataaatta catttagcca cgactacgtt gcacttccag ccaccacttc tccagctccg
    24661 cggcaaaggc ctggcggtca cgttgtgaaa ggctatctgg tccgccggtc tggatcccac
    24721 tggcacgtaa ggtatccata aaatcgcgca tcgtcaggcg ctcacgaatg gtcgctggcg
    24781 tgtaacgttc gccgcgcgga ttaagcgccg ctgcgccttt ctcgatggct tcagcagcca
    24841 aaggtatatc tgcggtgatc accaaatcgc ccgcttcaca ctgccggaca atttcgttat
    24901 cggcaacgtc gaaacctgcc gcgacgcgca gcgtacgaat aaatcgcgat ggcggcacgc
    24961 gtaaactctg gtttgctacc agtaccagcg gcatctgcat acgttccgcc gcgcgataca
    25021 aaatctcttt aattacattg ggacacgcgt cggcatccac ccaaattgtc ataaagtcat
    25081 cctttgttgg gtaatcctct attgtgtcgc gcttttgcct tccggcatag ttctgtttat
    25141 gcttctgcca gcgattatca aaacaatgaa tttcacggca ggagtgaggc aatggaaaag
    25201 aaaatcggtt ttattggctg cggcaatatg ggaaaagcca ttctcggcgg tctgattgcc
    25261 agcggtcagg tgcttccagg gcaaatctgg gtatacaccc cctccccgga taaagtcgcc
    25321 gccctgcatg accagttcgg catcaacgcc gcagaatcgg cgcaagaagt ggcgcaaatc
    25381 gccgacatca tttttgctgc cgttaaacct ggcatcatga ttaaagtgct tagcgaaatc
    25441 acctccagcc tgaataaaga ctctctggtc gtttctattg ctgcaggtgt cacgctcgac
    25501 cagcttgccc gcgcgctggg ccatgaccgg aaaattatcc gcgccatgcc gaacactccc
    25561 gcgctggtta atgccgggat gacctccgta acgccaaacg cgctggtaac cccagaagat
    25621 accgctgatg tgctgaatat tttccgctgc tttggcgaag cggaagtaat tgctgagccg
    25681 atgatccacc cggtggtcgg tgtgagcggt tcttcgccag cctacgtatt tatgtttatc
    25741 gaagcgatgg ccgacgccgc cgtgctgggc gggatgccac gcgcccaggc gtataaattt
    25801 gccgctcagg cggtaatggg ttccgcaaaa atggtgctgg aaacgggaga acatccgggg
    25861 gcactgaaag atatggtctg ctcaccggga ggcaccacca ttgaagcggt acgcgtactg
    25921 gaagagaaag gcttccgtgc cgcagtgatc gaagcgatga cgaagtgtat ggaaaaatca
    25981 gaaaaactca gcaaatcctg atgactttcg ccggacgtca ggccgccact tcggtgcggt
    26041 tacgtccggc tttctttgct ttgtaaagcg ccaaatctgc cgatttcaac cactcacgat
    26101 agtgactcat ttgtgggttc agcggcgcaa cccccacact aatccgtaaa gttacctgtg
    26161 gcgtattcgg caagcgtaac gtgtttagcc cttcacgcac ccgtaacatg gcggtaatgg
    26221 cgctctcagc tggcgtaccg gacatgatta ctgcaaactc atcgccgcca aaccgaccaa
    26281 tcacatcgct accgcgcagg gtaatttgta actgtcgggt aagcgccaca atcgcttcat
    26341 cgcccacatc atgaccccag gtatcgttga tgctcttgaa atggtcgata tcgataatca
    26401 gtaacgttgc atcgcgatta tgccgccgac agttatcaaa ttcattgcgt aacatagttt
    26461 cccaatgacg tcggttatac acgcccgtca tgccgtcgcg agtactcatg acctgcaacc
    26521 tgcgtttatg ttccgccagt ttggttgccg tctggtagct gacccagcca aacagcagag
    26581 gataaacgac aataatggga agggagagcc accattccag cggcgcacta ttgaacgaca
    26641 cggtaatgcc cgtaagctcg agggtgacaa ggcaggaaac caccatcaac accagacccg
    26701 cgacaaacag acgggggccg cctgccccca tcaaattcag acacataatc atcaacatag
    26761 cggtggaagg cagcacgttt acgcccatta cgcctaccca cattcccgct aatactgcat
    26821 cggtttttaa gttgtaaatt tcccggctaa gcggatcgac ggccctgctc gctatctgcc
    26881 aagctaaatg cggccagacg aacgcccagc cgaccaacac cagccaccac cagcccggcg
    26941 gcgggtgcga aaccagcgtt gaagcaatcg gtaagaacat gccagccagg ccaaccgcac
    27001 ggggtagtct gacgcgacgg gcgaagcgca gcccggaccg ctgatgttcg ttttgaggag
    27061 ttaaaggagg ttcctcccca tgcgccgccg cttttttgaa aaagttttca tcattcatta
    27121 tttttgggaa cattcagaaa caattttccc aaattataga gacggggcta aaggcagaag
    27181 tatgaaattt cgggtgagcc actttcgtag ctcacccgtt agcactatca ggtatcaggc
    27241 ggctttcttg aggcaggcac tcataaactt attacgatca tcacctttca gagattgttg
    27301 cgttgcttga ttattacatt cgcgcatctt ttgctactgt ggcgtcaaac ttttttcacc
    27361 aggcgcagac ttgctgttct tcaggcaatc actcatgtag gtcttacgag catccccttt
    27421 caacgcctgc gccgtcgcct gctgattaca ggaggtcata cgctgttgtt gtggggttaa
    27481 ggttctctcg gcagcgccga cggtggttaa aaaaaccaga ccaaaaagca aggtaaccag
    27541 taatgttatt ttcatagcac catccctctt catgttttaa ccatgagcgt ttgcgcccgt
    27601 gatctgccat taagtctggt tgctaacagc aaaaaaacca cccggcagcg aaaattcact
    27661 gccgggcgcg gttttatttc agccccagag cggctttcat ggtgtagaag agatcggtct
    27721 ggtcggtcag tccaacaaca ttggcggcat gcgggccata cgccgcaata cgcaactgac
    27781 tgccggtatg ttcttgtgaa tcctcttcgg aattcccgta actcatcacc atcactgcgc
    27841 catctttagt atttagcgcc tgagtgaggc ccggcgcttt ggtatccggc gcaacaacct
    27901 ggctggcgtg ggcgtgatca gcggtgacta tgaccagcgt gttaccctcc tttttagcga
    27961 attccagcgc ccgttgtacg gcttcatcga gatcgaccgt ctcgccaatt tgcccacaag
    28021 gattcgcagc gtgatcctgt ttatcgattg acgcaccttc aacttgcagg aaaaatcctt
    28081 tctcattttt actcaacaat tcaatggctt tgtcggtcat ctgtgccagg gttggtacac
    28141 tgtcattacg ttgcggatta ggcgtatagg tgactgcggg cttatcgata ttgccgtggt
    28201 acgttgcttt cggccctagc cagcgcactg gcatattgcc gtcagcaaac aggccaagca
    28261 ggggtttttg ctgattcgct tccgtcaccg aattcagtga ggcagcatcg ttcaccaact
    28321 gataaccacg cgcctgtgcc tattcacgca gcgtttttcc ctgccattca ccagcggttg
    28381 ccgtttcagc aaaggttttt gcgccgccgc caagcgtaac gtcggcacga gcgttaagca
    28441 gctgttcggt aatcgatcct tttccgcctt tttccagagc gttacccgga catttttcac
    28501 tggtcgcgct cggaccgtag catttgcgcg aagtcacatg tgccaccagc gcagcgggcg
    28561 tggcatcctg caactctgcg gtagaaacgt taccggtcgc cagacctgcg gcttttgcca
    28621 tttccagaat cgttgggtga tctttttcgt gaatatcgac gcccagcgcg ccgttatagg
    28681 ttttgacacc ggttgaccag gcggttgctg atgcagccga gtcggtgacg tagtccggtt
    28741 tgccagtttt tttattcagc gcatagtgag tgtattgccc ggtaagcggt aaggcatcga
    28801 tacctttaaa aaagccgccc gcaccttcgg cataattacg tgcggcagta atttccgaat
    28861 cccccatccc atcgccaatc agcaaaataa tattttttgc aggtttatcg ctaagagaat
    28921 cacgcagagc ggcggtctga tcacccgtta agcggcgagc accgccgggt gcagtaatat
    28981 cgccctgagc agcccggttt tccagaacag gcatttctgg tgtccgggct tttgtcacag
    29041 gggtaaacag taacggtaag agtgccagtg caatagtgct ttgtttcact ttattttctc
    29101 catgtacaaa tacattaaaa aataaaaaca aagcgactat aagtctcggc cgtgacaact
    29161 ttatgacagc tgttgaaaag attaactttt tactgacgag gatgcttcaa taacttcttt
    29221 acgtaatcgc gcagcagctc cgtatcgtcg tcaggaatgc tggcatcggg ctttacctcg
    29281 tacagcaccc cctctacctg atcaatcaac cgctgttggt cattttgcgc catattgcga
    29341 agcattgcag tgacgataat ctccaaagct tctacctgcg cacacagttc tttcgactct
    29401 tcttcttttt gggcaagctt aaataacaac tcagcaatga gatttttcat gtctgtattt
    29461 ccttatccaa agtatggaga agttaacatt atgtttcaca acttcatagt gtctggaagt
    29521 attattttta tgacaaagga aatactttgc gcaaatattt tttgtttgat taccagcgaa
    29581 acgtttctct tatttaaata attacccaca caaaatatag gcttttaatt atcaaccttt
    29641 attataaata aaa
//
LOCUS       DADLSA010000036        29573 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.036, whole genome shotgun sequence.
ACCESSION   DADLSA010000036 DADLSA010000000
VERSION     DADLSA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.036"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..427
                     /locus_tag="JN691_001621"
     CDS             <1..427
                     /locus_tag="JN691_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY7243091.1"
                     /translation="VNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(459..1295)
                     /locus_tag="JN691_001622"
     CDS             complement(459..1295)
                     /locus_tag="JN691_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase YeiG"
                     /protein_id="HAY7243092.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            1553..2221
                     /gene="folE"
                     /locus_tag="JN691_001623"
     CDS             1553..2221
                     /gene="folE"
                     /locus_tag="JN691_001623"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAY7243093.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            2238..3395
                     /locus_tag="JN691_001624"
     CDS             2238..3395
                     /locus_tag="JN691_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAY7243094.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            3537..4577
                     /gene="galS"
                     /locus_tag="JN691_001625"
     CDS             3537..4577
                     /gene="galS"
                     /locus_tag="JN691_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAY7243095.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            4857..5855
                     /gene="mglB"
                     /locus_tag="JN691_001626"
                     /pseudo
     CDS             4857..5855
                     /gene="mglB"
                     /locus_tag="JN691_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
     gene            5916..7436
                     /gene="mglA"
                     /locus_tag="JN691_001627"
     CDS             5916..7436
                     /gene="mglA"
                     /locus_tag="JN691_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAY7243096.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            7452..8462
                     /gene="mglC"
                     /locus_tag="JN691_001628"
     CDS             7452..8462
                     /gene="mglC"
                     /locus_tag="JN691_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAY7243097.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8533..9768)
                     /gene="preA"
                     /locus_tag="JN691_001629"
     CDS             complement(8533..9768)
                     /gene="preA"
                     /locus_tag="JN691_001629"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HAY7243098.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(9762..11000)
                     /locus_tag="JN691_001630"
     CDS             complement(9762..11000)
                     /locus_tag="JN691_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY7243099.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(11194..11433)
                     /locus_tag="JN691_001631"
     CDS             complement(11194..11433)
                     /locus_tag="JN691_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAY7243100.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(11436..12155)
                     /gene="sanA"
                     /locus_tag="JN691_001632"
     CDS             complement(11436..12155)
                     /gene="sanA"
                     /locus_tag="JN691_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAY7243101.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(12305..13189)
                     /gene="cdd"
                     /locus_tag="JN691_001633"
     CDS             complement(12305..13189)
                     /gene="cdd"
                     /locus_tag="JN691_001633"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAY7243102.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(13319..14014)
                     /locus_tag="JN691_001634"
     CDS             complement(13319..14014)
                     /locus_tag="JN691_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAY7243103.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(14011..14409)
                     /locus_tag="JN691_001635"
     CDS             complement(14011..14409)
                     /locus_tag="JN691_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAY7243104.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYILIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            14648..15595
                     /gene="dusC"
                     /locus_tag="JN691_001636"
                     /pseudo
     CDS             14648..15595
                     /gene="dusC"
                     /locus_tag="JN691_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
     gene            complement(15966..16049)
                     /gene="yohP"
                     /locus_tag="JN691_001637"
     CDS             complement(15966..16049)
                     /gene="yohP"
                     /locus_tag="JN691_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HAY7243105.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            16183..17709
                     /gene="mdtQ"
                     /locus_tag="JN691_001638"
     CDS             16183..17709
                     /gene="mdtQ"
                     /locus_tag="JN691_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HAY7243106.1"
                     /translation="MLLMYCHAKLKNISQHTVISAHRFLPDYSPMNRDSFYPAIACFP
                     LLLMLAGCAPMHETRQALSQQTPAAQVDTALPTALKNGWPDSQWWLEYHDNQLTSLIN
                     NALQNAPDMQVAEQRIQLAEAQAKAVATQDGPQIDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEWQTQAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVDGVETDINASKTRQQ
                     LNDVAGKMKIIEARLNALTNHQTKSLKLKPVALPKVASQLPDELGYSLLARRADLQAA
                     HWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFD
                     SGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDAL
                     RVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIQLTGALGGGYKR
                     "
     gene            17762..18523
                     /locus_tag="JN691_001639"
                     /pseudo
     CDS             17762..18523
                     /locus_tag="JN691_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(18640..19218)
                     /locus_tag="JN691_001640"
     CDS             complement(18640..19218)
                     /locus_tag="JN691_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7243107.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            19388..19975
                     /locus_tag="JN691_001641"
     CDS             19388..19975
                     /locus_tag="JN691_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAY7243108.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVQEVLLALTVILWGYGYRLF"
     gene            20149..21081
                     /gene="pbpG"
                     /locus_tag="JN691_001642"
     CDS             20149..21081
                     /gene="pbpG"
                     /locus_tag="JN691_001642"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAY7243109.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(21119..22834)
                     /gene="dld"
                     /locus_tag="JN691_001643"
     CDS             complement(21119..22834)
                     /gene="dld"
                     /locus_tag="JN691_001643"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY7243110.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            23030..25327
                     /gene="bglX"
                     /locus_tag="JN691_001644"
     CDS             23030..25327
                     /gene="bglX"
                     /locus_tag="JN691_001644"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAY7243111.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            25579..26496
                     /gene="osmF"
                     /locus_tag="JN691_001645"
     CDS             25579..26496
                     /gene="osmF"
                     /locus_tag="JN691_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HAY7243112.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            26503..27660
                     /gene="yehY"
                     /locus_tag="JN691_001646"
     CDS             26503..27660
                     /gene="yehY"
                     /locus_tag="JN691_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HAY7243113.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            27653..28579
                     /gene="yehX"
                     /locus_tag="JN691_001647"
     CDS             27653..28579
                     /gene="yehX"
                     /locus_tag="JN691_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HAY7243114.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            28584..29315
                     /gene="yehW"
                     /locus_tag="JN691_001648"
     CDS             28584..29315
                     /gene="yehW"
                     /locus_tag="JN691_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAY7243115.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQATLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(29296..29403)
                     /locus_tag="JN691_001649"
     CDS             complement(29296..29403)
                     /locus_tag="JN691_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAY7243116.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
BASE COUNT         7119 a         7384 c         7941 g         7129 t
ORIGIN      
        1 cgttaacaaa gctcgtattc agggcgtgga aaccgaactg aaaattccgt tcaacgatga
       61 atggaaactg tcgatcaact acacctacaa cgatggtcgt gatgtcagca acggcgaaaa
      121 caaaccgcta tccgatctgc cgttccatac tgctaacggt acgctggact ggaaaccgct
      181 ggcgctggaa gactggtcat tctatgtttc tggtcactat accgggcaga aacgcgccga
      241 cagcgcgacg gctaaaacac cgggcggtta taccatctgg aataccggcg cggcctggca
      301 ggtgactaaa gacgtcaaac tgcgcgcagg cgtgctgaac cttggcgaca aggatctcag
      361 tcgtgacgac tacagctata acgaagacgg acgtcgttac tttatggcag tggattatcg
      421 cttctgatga gaagatgccc ggcgaaccgg gcggactttc acttcagtaa atactgtgca
      481 tggaagcgca ggtgatcctc tataaaagag gcgatgaagt agtaactgtg atcatatccc
      541 ggctgaatac gcagcgtcat cggccacgct ttctggcgcg cggcttctgc caataccgca
      601 ggttgcaact ggtcggcaag aaactgatca ttatcgccct gatcgataag cgtcgggatc
      661 gcatcctgcg cgttactggc atacatcagc gcgcaactat cccattccag ccatgcattt
      721 ttgtcctcac ctaaataggt gctaaacgct ttgattcccc acgggacgct gcacggattc
      781 acaatgggcg caaaggccga aacgctggtg tatttacccg gatttttcag cgccataatc
      841 agcgcaccgt gaccacccat tgagtgaccg ctaatggcgc agcggtcgct gacattaaat
      901 tgcgactgaa ccagcgccgg taattcatcg cgcagataat catacatccg gtaatgcgtc
      961 gcccacggcg gttgcgtggc attaagataa aagcctgcgc cctggcctaa atcgtagcca
     1021 tcgtcgttgg caaccttttc gccgcgcggg ctggtgtctg gcatcaccag tacaatcccc
     1081 agttccgccg ctacccgctg ggcaccagcc ttggtggtga agttctcgtc attgcaggtt
     1141 aatccggaaa gccagtacag cactggtggc ggggtgtgat cacgaggggg tgggagaaag
     1201 atactgaacg tcatcgggca gtttaaggta ctggagtcgt gtctccagcg ttgctgccag
     1261 ccttcaaaac agcggtgctc ttcgagcatt tccatgcgtg gctccttgtt gtgttgtttg
     1321 caatttcttc atcataccgt ttattcaatg tgctgtgagt aactttcact tccgtatttg
     1381 cataacgatg ttttaacatc tgctgatgaa aggcagcggc aattacaata attatcgctg
     1441 tgaatactgg attatgtgcg ccgcctcacg cacaataatc aagctgtaaa tcagcttaat
     1501 aactttgccc ccacgcaggg cggaggcgtc acacctgcag gagaaatcat aaatgccatc
     1561 actcagtaaa gaagcggccc tggttcatga agcgttagtt gcgagaggac tggaaacacc
     1621 gctgcgcccg cccgtgcatg aaatggataa cgaaacgcgc aaaagcctta ttgctggtca
     1681 tatgaccgaa atcatgcagc tgctgaatct cgacctggct gatgacagtt tgatggaaac
     1741 gccgcatcgc atcgctaaaa tgtatgtcga tgaaattttc tccggtctgg attacgccaa
     1801 tttcccgaaa atcaccctca ttgaaaacaa aatgaaggtc gatgaaatgg tcaccgtgcg
     1861 cgatatcact ctgaccagta cctgtgaaca ccattttgtt accatcgatg gcaaagcgac
     1921 ggtggcttat atcccgaaag attcggtgat cggtctgtca aaaattaacc gtatcgtgca
     1981 gttctttgcc cagcgtccgc aggtgcagga acgtctgacg cagcaaattc ttattgcgct
     2041 acaaacgctg ctgggcacca ataacgtggc tgtctcgatc gacgcggtgc attactgcgt
     2101 gaaggcgcgt ggcatccgcg atgcaaccag tgccacgaca acgacctctc ttggtggatt
     2161 gttcaaatcc agtcagaata cgcgccacga gtttctgcgc gctgtgcgcc atcacaactg
     2221 attaaaaggc aggaaccatg gagcgcaacg tcacgctcga ttttgttcgc ggcgtcgcca
     2281 ttctggggat cctgctatta aacatcagcg cctttgggct accaaaggcg gcttatctca
     2341 atcccgcctg gtacgatgct attacgccgc aggatgcatg gacctgggca tttctcgatc
     2401 tcatcgggca ggtgaaattc ctcacgcttt ttgcgctgct gtttggtgct ggcctgcaaa
     2461 tgttgctgcc ccgtggcaga cgctggatcc agtcgcggtt aacgctgtta gtcttgttgg
     2521 gctttattca cggtttattg ttctgggacg gcgatattct gctggcttac gggctggtgg
     2581 gcttaatctg ctggcggctg gtgcgcgatg cgccatcggt aaaaagcctg tttaataccg
     2641 gcgtcatgct ttatctggtg gggcttggcg ttttgctgtt attggggctg atttccgaca
     2701 gccagaccag ccgcgcctgg acgccggatg catcggctat tttgtatgaa aaatactgga
     2761 agcttcacgg cggcgttgaa gcgatcagta atcgtgccga tggtgttggc aacagtttac
     2821 tggcactggg cgcacagtat ggctggcaac tggcagggat gatgctcatt ggtgcggcgc
     2881 tgatgcgcag cggctggctg aaagggcagt tcagcttacg tcactatcgt cgtactggtt
     2941 ttgtgctggt ggcgattggg gtgaccatta accttcctgc catcgccctg caatggcagc
     3001 tggactgggc gtatcgctgg tgtgccttct tacttcaaat gccgcgggaa ctgagtgcgc
     3061 cgtttcaggc gattggctat gcgtcgctgt tttatggctt ctggccgcaa ttgagccgct
     3121 ttaagctggt gcttgcgatc gcctgcgtcg gacggatggc gctgaccaac tatctattgc
     3181 aaacgctgat ttgtaccacg cttttttacc acctcggttt gtttatgcat tttgaccgcc
     3241 tggagctgct ggcgtttgtt attccggtat ggctggcgaa tatcctcttc tctgttatct
     3301 ggctgcgtta cttccgccag gggccggtgg aatggctctg gcgtcagtta actttgcgtg
     3361 ctgccggacc ggcaatatct aaaacatcaa gataacgatc tggatcacat tcgttaacaa
     3421 aacggctgta accgtttcca ttgctgtgac tcgattcacg aagtcctgta ttcagtgctg
     3481 acaaaatagc cgccagcaag cagtcattta ctgcaatctc ataacaggta gtgaatatga
     3541 tcaccattcg tgatgtagcg cgtcaggctg gcgtctctgt ggcaacggtt tcccgggtgc
     3601 tcaataacag cacgctggtc agtgccgaca cgcgtgaagc agtaatgaaa gccgtgagtg
     3661 agctggatta tcggccaaac gccaatgccc aggcgctggc aactcaggtt agcgacacca
     3721 ttggcgtggt ggtgatggac gtttctgatg cgtttttcgg cgcgctggta aaagcggtgg
     3781 atctggtcgc ccagcagcat cagaaatacg tgctaatcgg caatagctat catgaagcgg
     3841 aaaaagagcg tcacgccatt gaggtgttaa ttcgccagcg ttgtaatgcg ttgattgttc
     3901 actcaaaagc attgagtgac gatgaactgg cacaatttat ggataacatt cccggtatgg
     3961 tgttaatcaa ccgcgttgtg ccggggtacg cccatcgttg cgtttgcctg gataatctca
     4021 gcggtgcccg aatggcgacg cgcatgttgc tgaataacgg tcatcaacgt attggttatc
     4081 tttcttccag ccacggcatt gaagatgacg ccatgcgtaa agcaggctgg atgagtgcgt
     4141 tgaaagagca ggatattatt ccgccggaaa gctggattgg cactggtacg ccggacatgc
     4201 cgggcggtga ggcggcgatg gttgaactgc tggggcgcaa tctacaactt accgctgtat
     4261 ttgcttataa cgacaatatg gccgctggcg cactgacagc attaaaagat aatggcattg
     4321 cgattccgtt acatctctca atcatcggtt tcgatgatat tcccatcgcc cgttacaccg
     4381 acccgcaatt aacgaccgtg cgttatccca ttgcttcaat ggctaaatta gccaccgaac
     4441 tggccttgca gggggcagca ggcaatattg atcctcgtgc cagccactgt tttatgccga
     4501 cgttagtgcg tcgccattct gtcgcaacgc gccagaatgc ggcggcgatc actaactcaa
     4561 caaatcaggc gatgtaaccg ctttcaatct gtgagtgatt tcacagtatc ctaacaatgt
     4621 gatagctatg attgcaccgt tttaacgttg taacccgtat gtaacagtga ataatcactt
     4681 tcaccgaggt aatagcgtca taacaataat taaagccgtt ttctggagcg ttaccgggca
     4741 tggaagaacg aattttaaaa agtgagcttc ggcgttcagt aacacttcat taactctact
     4801 gccccgccga gcatttatct caagcactac cctgcataag aaaaaccgga gataccatga
     4861 ataagaaggt gttaaccctg tctgctgtga tggccagcat gttattcggt gccgctgcac
     4921 acgctgctga tactcgcatt ggtgtaacaa tctataagta cgacgataac tttatgtctg
     4981 tagtgcgcaa ggctattgag caagatgcga aagccgcgcc agatgttcag ctgctgatga
     5041 atgattctca gaatgaccag tccaagcaga acgatcagat cgacgtattg ctggcgaaag
     5101 gggtgaaggc actggcaatc aacctggttg acccggcagc tgcgggtacg gtgattgaga
     5161 aagcgcgtgg gcaaaacgtg ccggtggttt tcttcaacaa agaaccgtct cgtaaggcgc
     5221 tggatagcta cgacaaagcc tactacgttg gcactgactc caaagagtcc ggcattattc
     5281 agggcgattt gattgctaaa cactgggcgg cgaatcaggg ttgggatctg aacaaagacg
     5341 gtcagattca gttcgtactg ctgaaaggtg aaccgggcca tccggatgca gaagcacgta
     5401 ccacttacgt gattaaagaa ttgaacgata aaggcatcaa aactgaacag ttacagttag
     5461 ataccgcaat gtaggacacc gctcaggcga aagataagat ggacgcctgg ctgtctggcc
     5521 cgaacgccaa caaaatcgaa gtggttatcg ccaacaacga tgcgatggca atgggcgcgg
     5581 tagaagcact gaaagcacac aacaagtcca gcattccggt gtttggcgtc gatgcgctgc
     5641 cagaagcgct ggcgctggtg aaatccggtg cactggcggg cacagtactg aacgatgcta
     5701 acaaccaggc gaaagcgacc tttgatctgg cgaaaaacct ggccgatggc aaaggtgcgg
     5761 ctgatggcac caactggaaa atcgacaaca aagtggtccg cgtaccttat gttggcgtag
     5821 ataaagacaa cttggctgaa ttcagcaaga aataagactg attgggcgca gtcgtttact
     5881 gcgcccttat tgtatggcca acaaggtaaa attatatggt cagctcaacg actccgtctt
     5941 ccggggaata cttgttggaa atgagcggta tcaacaagtc ctttcctggt gttaaggcac
     6001 ttgataacgt taatttaaaa gtccggccac attctatcca tgcattaatg ggggaaaacg
     6061 gtgcaggaaa atcgacatta ttaaaatgcc tgtttggtat ttatcaaaaa gactccggca
     6121 ccattttatt ccagggtaaa gagatcgatt tccattccgc caaagaggca ctggaaaatg
     6181 gtatttcgat ggtacaccag gagttaaacc tggtattaca acgttcggtg atggataaca
     6241 tgtggctggg gcgatatccc accaaaggca tgtttgtcga tcaggacaaa atgtaccgcg
     6301 aaaccaaagc gatttttgat gaactggata ttgatatcga tccgcgtgcg cgcgtcggca
     6361 cattatccgt ttcgcaaatg cagatgattg aaatcgccaa agcgttttcc tataacgcga
     6421 aaattgtgat tatggatgaa ccgacttctt cgttaaccga aaaagaggtc aatcatctgt
     6481 tcactattat tcgtaaatta aaagagcgcg gctgcggtat tgtttatatc tcgcataaaa
     6541 tggaagaaat cttccagtta tgtgatgaag ttaccgtatt gcgcgacggt cagtggatcg
     6601 ccaccgaacc gctggcagga ctgacgatgg acaagatcat cgccatgatg gttgggcgtt
     6661 ctcttaatca gcgtttccct gacaaagaaa acaagccggg cgaagtcatc ctcgaggtgc
     6721 gtaacctaac gtcactgcgc cagccgtcga ttcgcgatgt ctcgtttgat ctgcataaag
     6781 gggagatcct cggtattgcc gggctggtgg gggcgaaacg taccgatatt gttgagacgt
     6841 tatttggtat tcgcgagaaa tcggctggca ccattacgtt gcacggcaaa cagatcaata
     6901 accataatgc caacgaagcc ataaaccacg gatttgcact ggtaactgag gagcgccgct
     6961 caacgggaat ttatgcctat ctggatattg gttttaactc gttaatttcc aatattcgca
     7021 actacaaaaa taaagttggt ttactggata actcgcggat gaaaagcgat acccagtggg
     7081 tgattgattc gatgcgggta aaaacgccgg gtcatcggac gcaaattggt tcgctctccg
     7141 gtggtaatca gcaaaaggtg attattggtc gctggctact aacgcaacca gaaatattaa
     7201 tgctcgatga accgacgcgc ggtattgatg tcggggcgaa gtttgaaatt tatcagttaa
     7261 ttgccgaact ggcgaagaaa ggcaagggga ttattattat ctcctctgaa atgcctgagt
     7321 tgttagggat aacagaccgt attctggtca tgagcaatgg tctcgtttcc ggaattgtcg
     7381 atacaaaaac aacaacgcaa aacgaaattc tgcgtcttgc gtctttgcac ctttaagatt
     7441 aggggcttcc catgagtgcg ttaaataaga aaagttttct tacttacctg aaagagggcg
     7501 gtatttatgt cgttctttta gttttgctgg cgattattat tttccaggac ccaacatttt
     7561 taagtctgtt gaacttaagt aatattctca cccagtcatc ggtgcgtatt attatcgcgc
     7621 tcggtgtggc agggttaatt gtcacccagg ggaccgatct ttctgctggt cgtcaggtag
     7681 ggctggcggc agtggtggct gcgacattat tgcagtccat ggataacgcc aacaaagtgt
     7741 tcccggaaat ggcgacgatg ccgattgcgc tggttattct gattgtctgt gccattggtg
     7801 cggtgatcgg tttgatcaac ggtctgatta tcgcttatct caacgtgacg ccgttcatta
     7861 ccacgctcgg cacgatgatc atcgtctatg gcatcaactc gctctattac gactttgtcg
     7921 gggcgtcgcc aatttctggt tttgacagtg gcttctctac ctttgctcag ggctttgtcg
     7981 cgctggggag tttccgtctc tcttacatca ccttctacgc gttgattgcg gtggcgttcg
     8041 tctgggtgtt gtggaacaaa acccgcttcg gtaagaacat ttttgccatt ggcggtaacc
     8101 cggaagcggc aaaagtatct ggtgtcaacg tcggcctgaa cctgctgatg atctacgcgt
     8161 tgtctggcgt gttctatgcc tttggcggga tgttagaagc cggacgtatc ggctctgcca
     8221 ccaacaacct cggctttatg tatgagctgg atgctatcgc ggcgtgcgtg gtaggcggtg
     8281 tatcgttcag cggcggtgtg gggacggtga ttggcgtggt gaccggggta attattttta
     8341 ccgtcatcaa ctatggcctg acgtatatcg gcgtaaaccc atactggcag tacatcatca
     8401 aaggggcgat tattatcttc gccgtagcgc tggattcact gaaatacgcg cgtaagaagt
     8461 gatgtgattg cggatattgc cggatgcgct gcatccggca atcggtgcca aatgccggat
     8521 gcgtctctgg ttttacaacg ttaccgggtg ttctttctcg cctttcttaa acttcacttc
     8581 cccgagctca atacaaccca ccgggcagac gtgaccacaa agcagacaac ccacacattt
     8641 ctcggtatta caatgcggtg tgcgggtttt ctcgctccat tccatcgcct ggtgaccgcc
     8701 gtcgtaacag gaaatataac agcgtccaca gccaacacat ttgtcaagat tgatacgggg
     8761 atagacaata taactgcggt ctaaatcttc cgccgggaca atattgttat tcgccagacc
     8821 taccatctcc tgcaacgaat caaatccctg atcggcgaga taatgcgata acccgctcgc
     8881 catatcttct acgatgcgat agccgtactg cataatgccg gtggtgacct gtaacgttgc
     8941 tgcgccgagc aataaaaact ccgccgcatc ttcccaggtt tcaatgccgc cgataccgct
     9001 gattgggaaa tcgcgcagtt ctggatgggt gcgcatttgc tgaatgaagc gcagggcgat
     9061 cggttttacc gctttaccgg aatatccgga aatactcgat ttaccgttaa cgatcggcat
     9121 accgattttc tgattaagat cgatattggt gatggattta acggtgttaa ttgccgcaat
     9181 gccatctgcg ccgccgcgct tcgccgccag cgccacttcg cacatatcac cgatattcgg
     9241 cgtcatcttc gccagcattg gcagcgtgga accccgtttc actgcccgac aatatttttc
     9301 taccagctcc gggctttgcc cgacatcgct acccatcgca tgagaagtca tttgcggaca
     9361 ggagaagtta cactcgatca tatccgcgcc agcttcttgc accaggcgcg ccagctcctc
     9421 ccattgctgc tcattttccc ccatgatcga agcgatcaat actttgtccg ggtaatcttc
     9481 cttcagccga cgcagggcgg tcagattctc ttccaacgga tgttcagcaa tctgctccat
     9541 atttttgaag ccgataaaac cggtatcttc tttcaccaga tgatcaaaac gcggcgagac
     9601 ttcgttggcg ataaaaaagc cgatcgtttt aaacaccaca ccgccccaac ctgtgtcgta
     9661 ggctttggca cacatctcat agcagttgcc taccggcgaa gaagagaggc agaacgggtt
     9721 gggaaagttc acgccgcaaa aagtaatcga aagatctttc gttaacatga gcaagctccc
     9781 tctaaatagt gatgaatcgc ctcggcggct tctttcccgg ttttcacggc atagaccacg
     9841 gttttgtcac cctcaacaat atcgccagca gcaaagactt gcggatcgcg ggtctgataa
     9901 ttttgtgttt tgatggtgtt acgctgctgc tctaactccg caaaggcatc cagtctggca
     9961 tgctgaccga cggcgagaat aattttatct gccgccatcg tcagttcgcc tgataaccgt
    10021 acatgcttaa acgtgacttt attgccttcc acggctactg gcgtgaatcc atcgatgatc
    10081 gaaacgccca gctcccgggc gctggcaaac tctttttcgc ttgccggaaa ctcatctaac
    10141 tcttcacgtg ctacgcaagt taccgcctga cagccgagaa ctttcagcgt gctggctacg
    10201 tccatcgcga catcaccgcc gccgataatt aatgcgcttt gtggaatgct gatatcgcct
    10261 tgcgcctgtc gtacacgttg caggaagtcg acggcaatct caacgtcgct atgctcaaac
    10321 agcggtagcc cggaaccgct tgataacccc acggtgacca gtaccgcgcg gttttccgct
    10381 ttcagttgct ccagggtgag tgttttaccg atttcgttat tgcacttaat ggtcacgccc
    10441 attttttcaa tacgggcgat ctctgcatcc agcactgatt gcggtaaccg gaattgcgga
    10501 ataccgttac gcaaccagcc accggggtgc gcttctttct cataaatcgt gacgtcataa
    10561 ccctggtttg tcagtgtcac actggcctgc aatccggcag gacctgcgcc aataatcgcg
    10621 actttgccga gcgttttagt accgggctga taaatttcca tcccggtttg ttgttcaaaa
    10681 tcagtaacaa aacgctgtaa gcggccgata tcaatgggag catcaacacc ggcacgggta
    10741 caaccgcttt ggcataattt ctccgtcggg cacactctgg cacaaacggc accgagggca
    10801 ttattttcgc gaattgtctc ggcagcgcct ttaaaattac gaaagtagat tgagcgaata
    10861 aatttccccg gatcggtctg tgccgggcaa gcctgactac agggagcgtc gtgacataat
    10921 aaacagcgag aggcttcttt aatcgccagt aacggcgtaa aagccggagt gagttcatcc
    10981 agataatttt gttgcggcat gtgatcttcc ttaatgctat gagtaattat ggcgaacgct
    11041 aaataaatat tgcgtgctga tattaaggct atgcgcgtgg ggaaatttaa actgtgaaag
    11101 gattcacaaa tagctgcggt tcagaatgtt atatccgcag cttactgagt gaagtaataa
    11161 ttattctcaa taattaacag aaaagagaaa atattaacga tagaaaataa gataaacaat
    11221 catgccgata gataaaattc cgaagccaat atatgccacc atataagcga agaagagtcc
    11281 gggatttttt gctcgccaga catacaccgg ttccggtgca tttttaaccc gtttatcaat
    11341 cgcgcctgcg cgccagcctt tccatgcttc gcggaaacaa aatatcggga taaggaaaaa
    11401 aacggcaacg acaaaaaact gctgtacatc cataactact ttccttgttt cttttgtaat
    11461 tcaagtaact gttcgggtgt aacggcggga tacccctgcg catcttccgg cacctggtgc
    11521 atagccggaa tagggaccag cggccctaaa aaacgcggtt cacgtttaaa aatataaagg
    11581 tcagccagcg caccgaaacg ggcggcaaat tcgcgaatac gtactgacag catatctttc
    11641 ggtgacggta cggcataaca ctgagcctga atccccatat gcagcgcaat aaataatgct
    11701 cgctcacagt ggaaacgttg ggtgataata atgaaatcat tagtatcgaa aactttgcgt
    11761 gtacgcacga tggaatccag cgtacgaaag cctgcgtaat cgagaacaat atctgatggg
    11821 tcgacaccag cagcgattaa atctttgcgc atggtcatcg gctcgttata actttgcaat
    11881 gcgttatcgc cgctcagtaa tagataattt accttaccgc tgttataggc attaatcgct
    11941 ccttgaatgc ggtagcgata atactgatta ataacgccgg tacgataata ttttgctgtg
    12001 ccgagcacca cgccgacctg gcggtagggg agatcctgca attcgtcgta gatataaggc
    12061 gcggttttcc agctcatcca gcgatcgagg ccgagcacag tcaacagcag caagccgatc
    12121 aggactaaca ggctgaggaa cacgcgcttt aacatgcagg agactcgagc taaggaaaat
    12181 ttccatcagg ctactttacc cgctggtttt gcgcaagaaa cggcaaactg tttgccggga
    12241 atttcgacac caggagaggg ctggcaagcc ggacaaggcg ttcacgccgc atccggcacc
    12301 aggcttaagc gagaagcacg cggtcgatac tgtgacagcc gagagctttc agcgttgcgg
    12361 aggtggcatc ccactgaatc aacggcgcat cggctttttc tgccagaacc gcgcgctgga
    12421 tatccgggta atcataaccc ttgagattca acagaattaa cgccccctgc aacggcggca
    12481 gagtcgggtt gaatgcggcg ttttcagcgt agctgccgct gaaaatacga ccgtctttac
    12541 attccagcgc gacaccgctt ggcgacttac tgtaaggcat gtgcgaacgg tttgccgctg
    12601 caatcgccgc ctgagaaagc gcatcaccag tcagcgcata gccgtgatcc tgctcgtcca
    12661 tcagcagcgt tttaatctcc agatctttcg gcccaaaggc atccggcaga tagtcacgca
    12721 gcgcgtgtgc ctcgcggccc ggcaaatgaa tacgcagatc cagaccgctg ttcagttcat
    12781 tcataaactg acggcagtga ccacaaggcg tatagttaac ggtgatggct gcaagcgctt
    12841 tttcaccact caaccaggcg tggctgatcg cgctttgttc ggcatgaacg gtttgctgca
    12901 ttgttgcacc aataaattcc atattggcac caaaatacca ggttccgctc acaccgcgcg
    12961 caattgcgcc aacattaaaa ttcgacaatg gcgtacgcgc acaggccgcc gccagcggaa
    13021 gtagtgcgaa tgccagcgcg tcttcgtcca gccccgttgc gctcttcagc gatgagactt
    13081 gctccccggt caacaaagcg gggaagtact tgtctgccag aataggttcc agtgcagatt
    13141 gcaagttatc cgcaagttgg gcaaaagcgg tttgaaaacg tggatgcatg gcgttgcctc
    13201 ataatcatgt aatgggataa cagtttacga acccgttatg gctttatatg tgatctgaat
    13261 ctcattaatt atgcaacacg tgaaacttac atcaaaaatg cgcgacgtat cgcaaatttt
    13321 agcccattac tgccagaata atcgggaaaa ggaacggtgc gatcagcgaa gtaattatcc
    13381 cgcataacac cagcgctagc gaactaaatg caccttcctg ataatccagc tcggcgcagc
    13441 gcgccgtacc gagggcgtgc gaggcagtcc ccattgccag accgcgcgca gctttggtac
    13501 gaatacgcat cgcattaagc aatgtatggc caaataccgc gccgaggatg ccgacgaaaa
    13561 tcacgcaaac tgcgctgatt gccggaatac cgccaatact gccgccaacc gccattgcaa
    13621 taggcgtggt gactgatttc ggcaggattg acgcggcgat ttccggtgaa gcgcccatca
    13681 acaatgccac ggaagtcccc gtcaccatcg caaccacgct gccgataaaa cagatggtga
    13741 tgatcgattt ccagcgcgcg cggatctggt gtagctgctc atataaagga taggccagcg
    13801 cgacgaccgc cggttgcagc agatcgttta gcacttcgct acctttaaag taactgtcgt
    13861 aagagatgcc ggtcagcatt aaaaaaggaa tgatcaccac cattgctacc agcaacgggt
    13921 taagcagggg aaatttatac cgtgccgcca gtttgcgggc ggcaaaaaag acaatcaacg
    13981 tcagcggtaa tgaccaccag atattcgcca tcattcttct gatcctttct gacctacgac
    14041 tttacgttca ccgtgtacca gttgtgaact ccagctcacc accagaaaaa ccaccagcgt
    14101 actgacagca caggaaacca ctaccgggcc aaactgcgcg cggagcaaat caaaatattg
    14161 catgacgcct acgccaatcg gcacaaacaa tagcgccata tagcgaatca gtatgtagca
    14221 ccccggattg acccatttcg ccggaagaat ttgcaaggcc agcaggacaa acaggatcag
    14281 catcccgatg atgctgcccg gaatggttac cggtagcagg gaagcaatga aaatgcctgc
    14341 atacaggcag gcataaatca ggacgaaagc gcgtaaatat tgccagataa tgttcagtgt
    14401 cttactcata atcaacaccc cagatgagat ggattcatca tacaattaat tcgtaaaacg
    14461 tgctaccgat cacattaaga aatcagcgct tattttttcg ctacccggta ttacgcaatg
    14521 agcagcgaac tcaggcatac atcaagcata gagagggatt gcagtttgca gcaatccctc
    14581 tttgcagacc tttaatccgc tgaccatagg cgctaacata gcgcctcatt tttttgcggg
    14641 tgatgatatg cgtgtgttac tggcaccgat ggagggagtg cttgactctc tggtgcgtga
    14701 attgctgacc gaagttaacg actacgatct gtgcatcacc gagtttgtcc gcgtggtgga
    14761 tcaactgctg ccggtaaaag tctttcatcg catttgccct gagctacaaa acgccagccg
    14821 gacaccatct ggtacgctgg tgcgcgtgca gttgttaggt cagttcccac aatggctggc
    14881 agagaacgcc gcccgtgcgg tggagttagg ttcctggggc gtggatctca attgcggctg
    14941 cccgtcgaaa acggttaacg gtagcggcgg cggggcgacg ttactcaaag atcctgaact
    15001 catctactag ggggcaaaag cgatgcgtga agctgtaccg gcgcatttgc ctgtcagcgt
    15061 gaaagtgcgt ctgggctggg acagcagtga gaagaaattt gaaatcgccg atgcggttca
    15121 acaggctggc gctacggagc tggtggtgca tgggcggacg aaagagcagg gttaccgcgc
    15181 ggagcatatt gactggcagg cgattggcga gattcgccag cggctgaata ttccggtgat
    15241 tgccaacggt gaaatctggg actggcagag cgcgcaacaa tgcatggcga tcagcggctg
    15301 cgacgcagtg atgattggtc gcggggcgct caatattccc aacctgagcc gggtggtaaa
    15361 atataacgaa ccgcgaatgc cgtggccgga ggtggttgct ttgctgcaaa aatatacccg
    15421 tctggaaaag cagggcgata ccgggttata tcacgttgcg cggattaaac agtggttgag
    15481 ttatttgcgt aaagaatacg atgaagcaac ggaattattt cagcatgttc gggtgttgaa
    15541 taactcccct gatattgcaa gggctattca ggaaattgat atcgagaaac tctaaaaacc
    15601 atagtgatgt ttttgacaat gtgttagctc tttatcaata ggccgcacgg ttgtttggtg
    15661 gcgtcaaaat cgccgaggcg ttccctgaag gccggggcag cccacatgga tgtgggctga
    15721 gggcgcgttt tacagggatg ttacctcgcg cccgacccgg tagccgtaag ggataagccg
    15781 agggcaccgc gcagcggcga ttttgttcgc cagagcccgg gggtgcaggg ggcggcggcg
    15841 attggtcgcc ccctgcgcgc tccttgcgcc agtgattata tgaagcaatg cacatgacag
    15901 gagcgcaaca ggatgagaga ctgaaatatt ctcgttattt tcggatctga tgacattaat
    15961 taattttaaa atatcatctt aaatacgcca gtcaccacca gtagcccaat caggaaaata
    16021 atcaatacag cccagagtat aattttcatt ggcgttcctt tatttgtgag tgctgaataa
    16081 gtataggaaa aaacgaagcc ctttgccgtt atccataaac gattaagata aatcggaaga
    16141 tatcatttac ttagtctata tttttctggg tatgtcgttt tattgctact gatgtattgc
    16201 cacgcgaaac taaaaaatat atcgcaacac acggtaatct ccgcgcaccg tttcttacct
    16261 gattattccc ccatgaatcg tgattccttt tatccagcca tcgcctgttt tccgctgtta
    16321 ctgatgctgg ccgggtgtgc gcctatgcat gaaacccgcc aggcgttaag ccagcaaacg
    16381 cccgctgcac aagttgacac cgcattaccc acggcgctga aaaatggttg gccagacagc
    16441 caatggtggc tggagtatca cgataatcag ctcacttcat taattaacaa tgcactacag
    16501 aacgcaccgg atatgcaggt ggctgaacag cgcattcagc tagctgaagc ccaggcgaag
    16561 gcagttgcca ctcaggatgg tccgcagatc gacttttcgg cggatatgga gcggcaaaaa
    16621 atgtcggcag aagggctaat ggggccgttt gctctgaacg atccggccgc aggtacgacc
    16681 ggtccgtggt acaccaacgg aacttttggc ttaacggcgg gctggcatct cgatatctgg
    16741 ggaaagaatc gggcggaggt tactgcccgc ctgggtacgg ttaaagcacg ggcggcggaa
    16801 cgcgagcaaa cccgccaatt gctggctggc agcgtagccc gcctgtactg ggagtggcaa
    16861 acccaggcgg cgttaaacac ggtcttgcag caaatagaaa aagagcagaa caccattatc
    16921 gcgaccgatc gccagctata tcagaacggg attacttctt cagttgacgg tgtggaaacc
    16981 gatattaatg ccagcaaaac ccggcagcag ctcaacgatg tcgcggggaa aatgaaaatt
    17041 attgaggcac ggttaaatgc actcacaaat caccagacta agtcattgaa gcttaaaccg
    17101 gtcgcgttgc cgaaagtggc aagccagctt cctgatgaac tggggtactc cttactggcc
    17161 cggcgggcag atttgcaggc ggcgcactgg tacgttgagt catcgttaag cactattgat
    17221 gcggcaaaag cggcatttta tcctgacatc aacctgatgg ccttcctgca acaggatgcg
    17281 ttgcacttaa gcgatctgtt ccgtcattcc gcgcagcaaa tgggcgttac ggcaggcctg
    17341 acgctaccta ttttcgatag tggtcgtctt aacgccaatc tcgatatcgc aaaagccgaa
    17401 agcaacttgt ctatcgccag ctacaacaaa gcggtggttg aagcagtgaa tgacgtggcg
    17461 cgggcagcca gtcaggttca gacactggcg gagaaaaacc agcatcaggc gcaaattgag
    17521 cgcgatgcct tgcgtgtggt aggtcttgcg caggcccgct ttaacgcggg catcattgct
    17581 ggttcccgcg tcagcgaagc cagaatcccg gcgctgcgtg agcgggccaa tggcctgtta
    17641 ttgcaagggc agtggctgga tgcctccatt caactcaccg gtgcgctggg cgggggttac
    17701 aaacgctgat gatatgcgcc ttctatactt aacgtttatt cagcgttaag tggagaactc
    17761 gatggcatag gtcgcgataa ttaccgcttc cgattcgggg atcggcaaag agtgcgcgtt
    17821 attactggcg cagcaggggt ttgatattgg cattacctgg cactcagatg aagaaggggc
    17881 aaaagatacc gcgcgtgagg tggttaccca cggcgtacgt gcggagatcg tgcaactgga
    17941 tctcggcaat ctgccagaag gtgcgcaggc gctggagaca ctcatccagc gatttgggcg
    18001 tattgatgtg ctggtgaata atgcgggtgc aatgaccaaa gcgccgtttc ttgatatggc
    18061 ttttgatgag tggcgcaaga tttttaccgt tgatgttgat ggtgcatttt tatgctcgca
    18121 aattgcggct cgtcagatgg tgaaataagg gcagggcggt cgcatcatca acattacgtc
    18181 ggtacatgaa catacgccgc tgccggatgc cagcgcctac acagccgcta aacatgcgct
    18241 cggcgggtta accaaaacga tggcgctgga gttggtcagg cataagattt tggtgaacgc
    18301 agtcgcgtct ggggcgatag ccacgccaat gaacggcatg gatgatagcg acgtgaagcc
    18361 cgacgcggag ccttcgattc ctctacggcg ttttggcgca acgcatgaga ttgccagctt
    18421 ggtggtgtgg ctttgttcgg agggcgcaaa ttacacgaca gggcagtcgt tgatagtgga
    18481 tggcggcttt atgttggcga atccgcagtt caacccagaa tagcgcagtg aggtgcgtgg
    18541 atatttcaat gggttaggcc agataagaca ccagcgtcgc atcaggcatg gaacacccgg
    18601 tgccggatgt ggagtgaatg cttatctgtg gcaactgctt tacgcctgat tatccggctt
    18661 tttcttcccg cgccgtttca gccaccagcg cacgcccacc accagaacca cgaccagaat
    18721 caaccagacc cagtgcttca aatgctggtc gagattgtgc aaccacggcg caatcacctg
    18781 accaccagcg taaccaatag tggtaaaaat caacgcccag gcaaatgcgc cgagaatatt
    18841 cagcggcaga aagattttcg gcggcagctg gctggcacca atcagcgtcg ggccaatcac
    18901 ccgaaagcca tacataaagc gcgtaccaat gacaaacaga tacggatggc gttggataag
    18961 tttctgcgcc cgctcaattt tatcctgatg tttcgagaaa cggcgtaaca gcttgccgcc
    19021 aaaccgccgc ccgcacagat agagcacctg gtcgccaatc atgccaccaa gcgccacaga
    19081 aagtaccacc agtgggaact ttaatagccc ctgatgtgcc gcaacgcctc ccagcaaagt
    19141 cacggtttca ccttccgcca ggctaccgat aaccagcgcg gcataaccat attgtgagat
    19201 gagtgtattg agatccatag gccagtctgt ctccctttgg ttgcctgtaa gcatacaccc
    19261 tgtgaatcat tcacgctgaa aaaggacata catcagacgg tgcaaattac atggatgaac
    19321 aaaaaataat cacaagctgg cttatacttg aactgtcagg ttcatgaact cacaacaagg
    19381 gggcggtatg agccatgtct ggggactgtt ttcccatccc gatcgtgaaa tgcaggtgat
    19441 taatcgcgaa aacgaaacga tttctcacca ttacacccac cacgttttgc tgatggcggc
    19501 gattccggtg atttgcgcct tcattggcac tacacagatt ggctggaatt ttggcgatgg
    19561 cactatcctg aagttatcct ggtttaccgg actggcgctg gctgtcttgt tttacggcgt
    19621 tatgctggct ggggttgcgg tgatggggcg ggtcatctgg tggatggcgc gcaattaccc
    19681 gcagcgtccg tctcttgcgc actgcatggt cttcgcgggc tacgttgcaa ctccgctgtt
    19741 tttaagcggt ctggtggcgc tttatccact ggtctggttg tgtgcgctgg tcggcacggt
    19801 ggcgctgttt tacaccggct acctgcttta ccttggtatt ccgagcttcc tgaatatcaa
    19861 caaagaggaa ggccttagct tctccagttc gacgctggcg attggcgtgc tggtacagga
    19921 agtgctgctc gccctaaccg ttatactatg gggctacggt tatcgtttgt tctagaaagt
    19981 tctttgacgt tgcattgctg gcgtaaatgt cagcaatgca gcatttactc acgattctcc
    20041 tttgacgatc gcactttttg ctcgttatga tggcgtcgct agcctcagta aatccttact
    20101 ccggcggtgc gcaacccgtg cgcgtgaacc actatctgaa tgctcatcat gccgaaattt
    20161 cgagtttctt tatttagcct ggccctgatg ctggctgtgc cttttgcacc gcaggccgtt
    20221 gcgaaaacgg cagccgctac caccgcttca caaccggaaa ttgcctccgg tagcgcgatg
    20281 attgttgatc tgaataccaa caaagtgatc tattcgaacc acccggatct ggtgcgtccg
    20341 attgcgtcta tcagcaaatt aatgaccgcg atggttgtgc tggatgcacg actgccgctg
    20401 gatgaaaaac taaaagtgga tatcagccag acgccggaga tgaaaggggt ctattcgcgc
    20461 gtacgactga atagcgaaat cagccgtaaa gatatgctgt tgctggcgct gatgtcttca
    20521 gaaaaccgcg cggcggcaag ccttgcgcac cattatcccg gtggttacaa agcctttatt
    20581 aaggcaatga atgcgaaagc gaaatcgctc ggaatgaaca acacgcgctt tgttgaacct
    20641 accggattgt cggtgcataa cgtttcaact gcccgtgact taaccaaact gctcattgcc
    20701 agcaaacaat atccgttgat cgggcagtta agtactaccc gggaagatat ggcaaccttc
    20761 tctaacccga cgtatacgct gccgttccgc aatactaatc atctggtgta tcgcgataac
    20821 tggaatattc agttaaccaa aaccggcttt accaatgcgg cgggccattg tctggtgatg
    20881 cgtacggtta tcaataataa accggtggcg ctggtagtga tggacgcgtt tggcaaatat
    20941 acccattttg ccgatgccag ccgcctgcgt acctggattg aaaccggtaa agtgatgcct
    21001 gtgccggcag cagcgttgag ctataaaaaa caaaaagccg cccaaatggc ggcggcgggg
    21061 cagacggcac agaacgatta agtgaattcg gatggcgata ctctgccatc cgtaattttt
    21121 actccacttc ctgccagttt ttccgtttac tggttttacc gatccccgga ttcatgctat
    21181 tggtcggatc gttctcgcga tagaacttct gcaacgtctc cggtgcttta tacaaatgac
    21241 cgacgttatg ctcggcaggg tactgcgcgc cgcgctgctg tagcagttcc agcatctgtt
    21301 cttttaacgc atgcacatcc acgccttttt tcactatgta atcctgatgg aagacatagc
    21361 acataaaatg gccgtaatag agcttgtgca ccagctggct gtcgatctcc ggcggtaaat
    21421 gctcatacca ctcggtgtcg ttacgccgca gagcgatatc cagtgccaga atgtcttcga
    21481 cttcatcgga atgcaccgcc tgataacgaa ttgctgcgcc cgcagcggcg aaacggtgta
    21541 aaaacgcttt gctaccttcc tccggcgtac agacaaagaa atcgccctcg gcctgtttga
    21601 aataatccac cagccacgat ttggcttcac cgacgccatc gcctgccatt tttaacagca
    21661 gatgatgctc gtatttatcg cgccagtttt tcatgcgcgg cggtaaatgg ctggggaaca
    21721 ggtgaccgaa tttttgcatt gcacggtcag taaaatgcgg acggaagaat ttcactttct
    21781 ccagcatcgc atcggtgcgt cccttgagat taaagaagaa cggcatcttg tcggtgccta
    21841 acttatcaat catcaggaag gtgtctttgc cgtatttttc cgcaatatcg tagatatccc
    21901 ggtgcatata ttccccggca accggcagat tttcgaagtt agccagaata tgacggcgga
    21961 tttcggtcag cacttccggc tggttggtgc cgatataaaa cacctgctga tttttttccg
    22021 cttcgaaggt atcaagacgt accgcaaaga cggccagctt cccggcgcaa ccagaagatt
    22081 caaataaccg atccggatcg gcgttataac gtgcgggcgt gtcggcttca atatcacgaa
    22141 cgcggtggac atagtcataa tcgtgggcgt gacgaccatc gtgacgcaca tcgtcatctt
    22201 tgatgcgatc gtcatccagc ttgctaagga tctgctccgg cgtttcgccc agatcaatcc
    22261 ccagatggtt caccagcgtc agtttgccat cttcatctat acgcgcgaat aacgacattt
    22321 cggtatacgc cgggccacgt tgcaccagcg agccgccgga gttgttacaa ataccgccga
    22381 tgaccgatgc gcctatacac gatgatccaa tcaccgaatg cggttcgcgt cccagcggtt
    22441 tgagggcttt ttccagcgaa tagagcgtgg tgcccggata ggccagcacc tgttcgccct
    22501 tgccgagaac gtgcagcttg tcgagacgca gggtgctgat gatcacgata tcgcgatcat
    22561 agtcgttacc gtttggcgtc gatccttcgg tcaggcctgt attggcagcc tgcatcagaa
    22621 taattttgtc ggcggtgacg caggctttca gcacccgcca caattccagt agtgagccag
    22681 ggaaaacgac agccagcgcg tcgccctgac cagaacggaa gcccttgcga tagcgggccg
    22741 tttttgcggg atcggtgagc aggtgtgaat gaccgaccag acgggcaagt tcattcaaaa
    22801 aggctttatt atcagttgtt gtcatggaag acattttcca ctccttgtgg tggcgaaaaa
    22861 atatcacaaa ctagaatagc gccttgtatg acaattcagc gagtgaatat tttgaaaatt
    22921 cagacaataa actgagcatt ctttttctct ctccatcatg cttatggcaa actacggttt
    22981 ttgcaccatt ttcgctgcca cgtcgggcaa caaaggaaga aaaatccata tgaaatggct
    23041 atgttcagta ggaatcgcgg tgagtctggt cctgcagcca gcactggcgg atgatttatt
    23101 cggtaaccat ccattaacgc ctgaagcgcg ggatgcgttc gtcaccgatc tgctcaagaa
    23161 aatgacggtt gatgagaaaa ttggtcagct gcgcttaatc agcgtaggcc cggataatcc
    23221 gaaagaggcg atccgcgaga tgatcaaaga cggtcaggtt ggggcgattt tcaacaccgt
    23281 aacccgtcag gatatccgcg ccatgcagga tcaggtgatg gaattaagcc gcctgaaaat
    23341 tcctcttttc tttgcttacg acgtgctgca cggtcagcgc acggtgttcc cgattagcct
    23401 cggtctggcc tcgtctttta acctcgatgc ggtgaaaacg gtcgggcgtg tctctgctta
    23461 tgaagcggca gatgatggcc tgaatatgac ctgggcaccg atggtcgatg tctcgcgcga
    23521 tccgcgctgg ggacgtgctt ccgaaggttt tggcgaagat acgtatctca cctcaataat
    23581 gggtaaaacc atggtggaag cgatgcaggg taaaagcccg gcagatcgtt actcggtgat
    23641 gaccagcgtc aaacactttg ccgcatacgg cgcggtagaa ggcggtaaag agtacaacac
    23701 cgtcgatatg agtccgcagc gcctgtttaa tgattatatg ccgccgtaca aagcggggct
    23761 ggacgcaggc agcggcgcgg tgatggtggc gctgaactcg ctgaacggca cgccagccac
    23821 ctccgattcc tggctgctga aagatgttct gcgcgaccag tggggcttta aaggcatcac
    23881 cgtttccgat cacggtgcaa tcaaagagct gattaaacat ggcacggcgg cagacccgga
    23941 agatgcggtg cgcgtggcgc tgaaatccgg aatcaacatg agtatgagcg acgagtacta
    24001 ctcgaagtat ctgcctgggt tgattaaatc cggcaaagtg accatggcag agctggacga
    24061 tgctgcccgc catgtactga acgttaaata tgatatgggg ttgtttaacg acccatacag
    24121 ccatttgggg ccgaaagagt ctgacccggt ggataccaat gccgaaagcc gcctgcaccg
    24181 taaagaagcg cgtgaagtgg cgcgcgaaag cctggtgttg ctgaaaaacc gtctcgaaac
    24241 gttaccgctg aaaaaatcgg ccaccattgc ggtggttggg ccactggcgg acagtaaacg
    24301 tgacgtgatg ggcagctggt ccgcagccgg tgttgccgat caatccgtga ccgtactgac
    24361 cgggattaaa aattccgtcg gtgaaaacgg taaagtgctg tatgccaaag gggcgaacgt
    24421 taccagtgac aaaggcatta tcgatttcct gaatcagtat gaagaagcgg tcaaagtcga
    24481 tccgcgttcg ccgcaagaga tgattgatga agcggtgcag acggcgaaac aatctgatgt
    24541 ggtggtggct gtagtcggtg aagcacaggg gatggcgcac gaagcctcca gccggaccga
    24601 tatcactatt ccgcaaagcc aacgtgactt gattgcggcg ctgaaagcca ccggtaaacc
    24661 gctggtgctg gtgctgatga acgggcgtcc gctggcgctg gtgaaagaag atcagcaggc
    24721 tgatgcgatt ctggaaactt ggtttgcggg gactgaaggc ggtaatgcaa ttgccgatgt
    24781 attgtttggc gattacaacc cgtccggcaa gctgccaatg tccttcccgc gttctgtcgg
    24841 gcagatcccg gtgtactaca gccatctgaa caccggtcgt ccgtataatg ccgacaagcc
    24901 gaacaaatac acttcgcgtt attttgatga agctaacggg gcgctttatc cgttcggcta
    24961 tggtctgagc tataccactt tcaccgtctc tgatgtgaaa ctttctgcgc cgaccatgaa
    25021 gcgtgacggc aaagtgacgg ccagcgtgca ggtgacgaac accggtaagc gcgagggggc
    25081 cacggtagtg cagatgtact tgcaggatgt gacggcttcc atgagtcgcc ctgtgaaaca
    25141 gctgaaaggc tttgagaaaa tcaccctgaa accgggcgaa actcagactg tcagcttccc
    25201 gatcgatatt gaggcgctga agttctggaa tcaacagatg aaatatgacg ccgagcctgg
    25261 caagttcaat gtatttatcg gcactgattc cgcacgcgtt aagaaaggcg agtttgagtt
    25321 gctgtaatgc tttagtaagg gcgcgacgtt tgcgcccttt gcttgatgtt catatcttaa
    25381 cttgctgaat tttcgccctt tgtaggccgg ataaggcgct cgcgccgcat ccggcaggaa
    25441 acgccggacc gaatatgcct cttaagtatt tatctgattc tgcaactgaa tccttccgct
    25501 caggctaact ctgtcattat caactatgct tttctcttaa ttcgctgaaa acaacagtaa
    25561 aagaatgagg aaagcatcat gccactctta aagctctggg caggttcact ggttatgttg
    25621 gcagccgtga gcctgccgct acaggcggct tcccccgtta aagtcggttc aaaaatcgat
    25681 accgaaggcg cgctgctcgg caacatcatc ttgcaggtac tcgaaagcca cggcgtaccg
    25741 acggtgaata aagtgcaact cggaacgact cctgtggtgc gcggggcgat tacttccggt
    25801 gaactggata tctatccgga atataccggc aatggcgctt tcttctttaa agatgaaaac
    25861 gatgcagcgt ggaaaaatgc cgggcagggc tacgagaaag tcaaaaagct cgatgcggag
    25921 cacaacaagt taatctggct gacgcccgcg cctgcaaata acacctggac tatcgccgtg
    25981 cgtcaggatg tggcagagaa aaacaaactc acttcgcttg ccgacctgag tcgttatctg
    26041 caagagggcg gcaccttcaa actggcagcc tcggcagagt ttatcgaacg cgccgatgcg
    26101 ttacccgcgt ttgaaaaagc ctatggcttt aagctcggtc aggatcagtt gctgtcactg
    26161 gctggtggcg acacggcggt gacgattaaa gccgctgccc agcaaacctc tggcgttaat
    26221 gccgcaatgg cttacggcac tgacggtccg gtcgcggcgc tggggctgca aaccttaagc
    26281 gatccgcaag gcgtgcaacc tatctacgcg cctgcaccag tggtgcgtga atcggtgttg
    26341 aaggagtatc cgcaaatggc acagtggcta cagccagtct tcgccagcct cgatgcaaaa
    26401 acattgcagc aactgaatgc cagcattgca gtggaaggac tggatgccaa aaaagtggct
    26461 gccgactacc tgaaacaaaa agggtgggcg aagtaatttc ccgtgactta tctacgtatt
    26521 aatcctgttc tggcgctgct gttgttgctg acggcaatcg cagcggcgct gccgtttatc
    26581 agttacgcgc ctaatcgttt agtctcgggt gaagggcgtc atctctggca actgtggccg
    26641 caaacgatct ggatgctggt gggcgttggt tgcgcctggc tgacagcctg ttttgtcccc
    26701 gctaaaaaag gcagcatttt tgcactcatt ctggcgcaat tcgtcttcgt attgttggtg
    26761 tggggagctg gaaaggcggc gacccaactg gcgcaaaatg gcagtgcgct ggcgcgtacc
    26821 agcctcggca gtggtttctg gctggctgcg gcgctggcat tgctggcctg tagcgatgcc
    26881 atccgccgaa tctccacgca tccgctgtgg cgctggttgt tgcatatgca gattgccatt
    26941 attccgctgt ggttgctgta ctccggcacg cttaacgatc tctcactaat gaaagaatac
    27001 gccaaccgtc aggatgtgtt tgacgacgcg ctggcacaac atctgacgtt gctgtttggt
    27061 gcggtgctgc ctgcgttagt gattggtgtg ccgttgggca tctggtgcta cttttccact
    27121 gcgcggcagg gggcaatttt ttctctgctc aatgtcattc agaccgtgcc ttcggtggcg
    27181 ctctttggcc tgttgattgc gccgcttgcc gcgctggtta cggcctttcc gtggctgggg
    27241 aagctcggca tagcaggaac cggaatgaca cccgcactga ttgcgctggt gctctatgcc
    27301 ttgctgccgc tggtgcgcgg cgtggtagtc ggtttgaacc agatcccgcg cgatgtgctg
    27361 gagagcgcca gagcgatggg gatgagcggg gcgcagcgtt tcctgcatgt tcagttaccg
    27421 ctggcgttac cggtatttct gcgcagcctg cgggtggtga tggtgcaaac tgtaggtatg
    27481 gcggtgattg cggcgttaat cggcgcaggc ggttttggtg cgctggtttt ccaggggctg
    27541 ctaagcagcg ccattgattt agtgttgctg ggggtgatcc cggtaattgt tctggcggtg
    27601 cttaccgacg cgctgttcga tttgcttatc gcactgctga aggtgaaacg taatgattga
    27661 atttagccat gtcagcaaac tgttcggcgc acaaaaagcc gttaacgatc tcaatctcaa
    27721 ttttcaggaa gggagttttt cggtgctgat tggcacatct ggctccggca aatccaccac
    27781 cctgaaaatg attaaccgcc tggtggagca tgacagcgga gtgatccgct ttgccggaga
    27841 agaaattcgc tcgctgccag tactggagtt gcgccgccgg atgggctatg ccattcaatc
    27901 tattggcctg ttcccacact ggagcgtggc gcaaaacatc gctaccgtgc cgcaattaca
    27961 aaaatggtcg cgggcgcgga ttgacgatcg tatcgacgaa ttaatggcgc tactggggct
    28021 ggagtcaaat ttgcgtgagc gttatccgca tcagctttcc ggtggtcagc agcaacgtgt
    28081 gggagtggcg cgtgcactgg ctgccgatcc gcaagtctta ctgatggatg aaccttttgg
    28141 cgcactggac ccggtaacgc gcggcgcgtt gcaacaagag atgacgcgca ttcaccgttt
    28201 gctggggcgc actattgtgc tggtcactca tgatattgat gaggcgctac ggctggcaga
    28261 acatctggta ttgatggatc acggtgaagt ggtgcagcag gggaatccgc tgacgatgct
    28321 gactcgtccg gcgaatgatt ttgtccgcca gttttttgga cgtagtgaac tgggggtgcg
    28381 cctgctttcg ttacgtagcg tggcggatta cgtgcgtcgc gaagaacggg cagaaggtga
    28441 ggcactggca gaagagatga cgctacgcga tgcgctctcc ctgtttgtcg cgcggggatg
    28501 cgaggtgctg ccggtggtga acacgcaggg ccagccttgc ggcacgctgc attttcagga
    28561 tctgctggtg gaggcgtaag cgtatgaaga tgttgcgcga tccgctgttc tggctcattg
    28621 ctctttttgt ggcgctgatt ttctggctgc cttacagcca gccgctgttt gctgccttgt
    28681 tcccacaact gccacgaccc gtttatcagc aagaaagttt tgcagctctg gcactggctc
    28741 atttctggct ggtgggaatt tcgagtttgt ttgcggtgat cattggcaca ggtgccggaa
    28801 ttgctgttac tcgcccgtgg ggcgcggaat ttcgcccact ggtggaaact attgccgccg
    28861 ttggacagac ttttccgcct gtcgcagtgc tggcgattgc cgttccggtg atcggctttg
    28921 gtctgcaacc agcgattatc gccttgatcc tttacggtgt gctgcccatc ctgcaggcga
    28981 cacttgccgg gctgggagcg attgatccca gcgtgacaga agttgcgaaa ggtatgggaa
    29041 tgagtcgagg tcagcgactg cgtaaggtcg agctaccgct ggcggctccg gtgattctgg
    29101 cgggcgtgcg aacttcggtg attatcaaca ttggtacggc gacgatcgcc tcaacggtag
    29161 gggccagtac gctgggtacg cccatcatca tcgggcttag cggatttaat accgcgtatg
    29221 tgatccaggg ggcgttactg gtggcactgg cggcgatcat cgcagaccgc ctgtttgaaa
    29281 ggctggtgca ggcgcttagc cagcacgcaa aataaaggta taacctgcga gcatgacgcc
    29341 accaattccg cctaacgcca taaacaggaa cagggcgatg accccaattt tagctatgcg
    29401 cataatgcac tccttatgtt aacgaaagga ttgtacagta aagcgcattt gttaacgaat
    29461 cattaaatac cgagtgggaa aatatcatgg ccttgttctt gccaactggt gagttgctgc
    29521 tgttgggcgg agtgaaccgc cccgggtttc ctggagagtg ttttatctgt gaa
//
LOCUS       DADLSA010000037        29128 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.037, whole genome shotgun sequence.
ACCESSION   DADLSA010000037 DADLSA010000000
VERSION     DADLSA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.037"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(1..845)
                     /gene="gsiB"
                     /locus_tag="JN691_001650"
                     /pseudo
     CDS             complement(1..845)
                     /gene="gsiB"
                     /locus_tag="JN691_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
     gene            complement(865..2736)
                     /gene="gsiA"
                     /locus_tag="JN691_001651"
     CDS             complement(865..2736)
                     /gene="gsiA"
                     /locus_tag="JN691_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAY7243117.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQDPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEYAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCEVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(2723..3688)
                     /gene="iaaA"
                     /locus_tag="JN691_001652"
     CDS             complement(2723..3688)
                     /gene="iaaA"
                     /locus_tag="JN691_001652"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY7243118.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            3892..5127
                     /gene="moeA"
                     /locus_tag="JN691_001653"
     CDS             3892..5127
                     /gene="moeA"
                     /locus_tag="JN691_001653"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAY7243119.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            5127..5876
                     /gene="moeB"
                     /locus_tag="JN691_001654"
     CDS             5127..5876
                     /gene="moeB"
                     /locus_tag="JN691_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAY7243120.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(6055..6717)
                     /gene="fsa"
                     /locus_tag="JN691_001655"
     CDS             complement(6055..6717)
                     /gene="fsa"
                     /locus_tag="JN691_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY7243121.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            6848..7747
                     /locus_tag="JN691_001656"
     CDS             6848..7747
                     /locus_tag="JN691_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAY7243122.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLPAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            7753..10185
                     /locus_tag="JN691_001657"
     CDS             7753..10185
                     /locus_tag="JN691_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAY7243123.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            10331..11146
                     /gene="ybiV"
                     /locus_tag="JN691_001658"
     CDS             10331..11146
                     /gene="ybiV"
                     /locus_tag="JN691_001658"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAY7243124.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            11298..12563
                     /locus_tag="JN691_001659"
     CDS             11298..12563
                     /locus_tag="JN691_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HAY7243125.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPNRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(12804..14396)
                     /locus_tag="JN691_001660"
     CDS             complement(12804..14396)
                     /locus_tag="JN691_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAY7243126.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            14615..15535
                     /gene="ldtB"
                     /locus_tag="JN691_001661"
     CDS             14615..15535
                     /gene="ldtB"
                     /locus_tag="JN691_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7243127.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDLVVLDEAIKNRSGMPVRLN"
     gene            complement(15593..16711)
                     /locus_tag="JN691_001662"
     CDS             complement(15593..16711)
                     /locus_tag="JN691_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAY7243128.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAVGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(16708..17175)
                     /locus_tag="JN691_001663"
     CDS             complement(16708..17175)
                     /locus_tag="JN691_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HAY7243129.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            17361..17489
                     /gene="mntS"
                     /locus_tag="JN691_001664"
     CDS             17361..17489
                     /gene="mntS"
                     /locus_tag="JN691_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAY7243130.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            17761..19347
                     /locus_tag="JN691_001665"
     CDS             17761..19347
                     /locus_tag="JN691_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY7243131.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETSFVQSKETSCYL
                     YTMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQV
                     PFMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITN
                     FQLQKVDYNHLGTDIFDPKP"
     gene            complement(19395..19910)
                     /gene="ompX"
                     /locus_tag="JN691_001666"
     CDS             complement(19395..19910)
                     /gene="ompX"
                     /locus_tag="JN691_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAY7243132.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(19963..20028)
                     /locus_tag="JN691_001667"
     CDS             complement(19963..20028)
                     /locus_tag="JN691_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243133.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            20263..21150
                     /gene="rhtA"
                     /locus_tag="JN691_001668"
     CDS             20263..21150
                     /gene="rhtA"
                     /locus_tag="JN691_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAY7243134.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            21449..21952
                     /gene="dps"
                     /locus_tag="JN691_001669"
                     /gene_synonym="pexB"
     CDS             21449..21952
                     /gene="dps"
                     /locus_tag="JN691_001669"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAY7243135.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            22356..23102
                     /gene="glnH"
                     /locus_tag="JN691_001670"
     CDS             22356..23102
                     /gene="glnH"
                     /locus_tag="JN691_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAY7243136.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            23241..23900
                     /gene="glnP"
                     /locus_tag="JN691_001671"
     CDS             23241..23900
                     /gene="glnP"
                     /locus_tag="JN691_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAY7243137.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            23897..24619
                     /gene="glnQ"
                     /locus_tag="JN691_001672"
     CDS             23897..24619
                     /gene="glnQ"
                     /locus_tag="JN691_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAY7243138.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVIQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            24880..27105
                     /gene="ybiO"
                     /locus_tag="JN691_001673"
     CDS             24880..27105
                     /gene="ybiO"
                     /locus_tag="JN691_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAY7243139.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVTELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRVPVQTYQVLPAPGAPPAEPLPPGEPT
                     L"
     gene            complement(27102..28028)
                     /gene="rlmF"
                     /locus_tag="JN691_001674"
     CDS             complement(27102..28028)
                     /gene="rlmF"
                     /locus_tag="JN691_001674"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAY7243140.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            28304..28564
                     /locus_tag="JN691_001675"
     CDS             28304..28564
                     /locus_tag="JN691_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7243141.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            28829..>29128
                     /locus_tag="JN691_001676"
     CDS             28829..>29128
                     /locus_tag="JN691_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY7243142.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         7018 a         7767 c         7305 g         7038 t
ORIGIN      
        1 tatcgaacgg cttttgcgtc acgttcatac tgatataacg ctgcataatt gacggactgg
       61 ccatcaactc gatatttttg tttttctcca gcagtgtggc ctgctcgtag ggaatgggga
      121 aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg ggtgttgtta tccgccaccg
      181 gacgccaggt tatgctgtcc agtttgggca atcctggctg ccagtaaccc gcgaattttt
      241 tcaccttcac aaaatcggtc tgattccagg tatccagttc atactggtcc ggttcccacc
      301 ggatgaaaac caatctcctt gccatatttt tccagcgctg tcggtgaaat catcgcggtc
      361 gccggatggg caagaatatt aataaacgct gagaacggct gtttgagggt aatctttacc
      421 gttgtcggat cgatcgcctc cgttttggca atattcttat acaggttata gcgtttaaga
      481 tgattcgccg gatcgctggc ccggtccaga ttcgctttta ccgccactgc gttgaaatcg
      541 gtgccatcct ggaatttaat tccttcccgc aatttcacgg tgtaagtaat gccgtcatcg
      601 gaaacggtat aactctccgc cagcacgttt ttcagtttca tctctttatc cagaccgaac
      661 agcccctagt aaaacgattt cgctacggcc tgagataacg tgtcattcgc gtcatacgga
      721 tcgagcgtgg tgaaattcga tcctaccgcc accaccacat ctttggcagc gaatgcacaa
      781 gatgccatca acgctgtcgc aatgcccagc gccactaacc cactacggtg tacagctctt
      841 gccattttat tctccgcctg aatgttatct acgcatgaat gcgtattctg attgtggttg
      901 tgcgacgtaa tgccccggcc cgacgcattg caacgagacg gctgccacct cttcgccacg
      961 cagatgaata ttgctgggaa gatcgtccga cagcagtaca cgctgcggtc gttgtcggga
     1021 cggttcagcg accggaactg ccgccagtaa tttacgcgta taaggatgct gcgggttttc
     1081 gaagacctcg caccgtgaac caatttcaac aatttgcccg agatacatca ccgccacacg
     1141 atgactaatc cgctctacca cagccatatc gtgggagata aacagatacg caatgccgaa
     1201 atcacgctgg agatcgagca acaagttgat aatctgcccg cgaatagaaa catccagcgc
     1261 cgaaacggct tcgtcggcaa tgatcacttt tggattcaat gccaacgcgc gagcaatgca
     1321 gatgcgctgg cgctgaccgc cggaaaactc atgcgggtaa cgccaggcat attcaggtaa
     1381 caggcccacg cgctccagca accacgcaac gcgtgcagcc gcgtctttac ctggcaataa
     1441 accgtgtaca cgcagcggtt cgataatcga atcaccgatg gtctgacgtg ggtccagcga
     1501 agcgtaaggg tcctgaaaaa taaactgaat atcccggcgc aatgcctgaa gtttgccagg
     1561 tgacaaggta tcgattcgct gaccgttaaa gataatttcg ccgccctgcg attcgaccag
     1621 gcgcagcaac gcccgcccgg tagtggattt accgctgcca gactcgccca ccagcgatag
     1681 cgtttcgcca ggccagagat caaaactgac tttctcaacg gcatgcactt cccgcgttac
     1741 gcgattcaac aaaccgctgc gcaaagggaa acgggtgaca agattacgca cccgtaaaac
     1801 aggttcgcca tccaccaccg ttttctgctc gatggggggg tcctgtttcg ctggatgttc
     1861 aagcgatatc aatgggaaac gtcggggata atctaaccct ttcatcgcac caagttgcgg
     1921 aacagcagct aacagcgcac gggtgtaagg atgttgcggt gcatgaaaaa tctgttcgac
     1981 ggtacccgtt tccaccgcct cgccctgata catcaccagt acccgatcgg caatctctgc
     2041 caccacgccc atatcgtgag tgataaagat aacgcccatc gacatctctt tttgcaatac
     2101 tttgattaat tgcaggatct gcgcctgaat agtgacatcc agcgcggtgg ttggctcatc
     2161 ggcaatcagc accgccgggc ggcatgacag cgccatcgca atcatcactc gctggcgcat
     2221 cccgccagag agttgatgcg aataacgtga aagaatggtt tgtgcctccg gaatgcgtac
     2281 ctgatccagc atccgcttcg cctcgaccat cgcttcttca cgactggcgt tctgatgcag
     2341 acgaattgat tcggcaatct gttcacccac agtaaatact gggttcagcg atgtcatcgg
     2401 ctcctgaaat atcatcgcca tatccgcacc gcgcacatgg cgcatttgtg cagcgctctg
     2461 ctcgctaagt tcaatcacat cacgactgcg ccgccgcaac agcattttat cgcactgtac
     2521 taaaccgccc gcctgttcca acagacgcat caatgccagc gcagtcactg acttaccgga
     2581 gccggattcg cctacaattg ccagcgtctc accgcgttgc agactaaaag agagattgcg
     2641 gaccgcagct attttctgct ggtcctgcat aaaggcaata ttaagatttt caaccgccag
     2701 cacattaccg gcatcaagtt catcactgtg tggcaacggt gtcccctttt tcacggtaga
     2761 taccggtggt tggcgtatcg cctgcgtagc cccaggcgcg atacattcct tcggtgttaa
     2821 acggtagcgc gacattccct tcatggtcga tagcgattaa gccaccgcta ccgccaagcg
     2881 cagggagttt ttccattact acccgctcgc aggcttccgc gagacttaat ccgccgtaat
     2941 ccattaacgc ggcgatgtca tatgccgcca gcgcgcggat gaagacttcg cctgtgccgg
     3001 tacaagaaac cgccacactg gcgttattgg cgtagcatcc ggcacccact aagggactat
     3061 cgccaactcg tccgggtaat ttattggtca ttccgcctgt ggacgtggct gccgccagat
     3121 tgccgtctaa atccaacgcc acggccccca cggtgcccat tttctgtttt tcatccagtg
     3181 gcgcaccgct atggtcgagg actgttgccc cttcctcgcg cgctgccagt agttgttcat
     3241 aacgcaaagg cgtggagaaa atctccggtg agacgcgctc catgccatga gcaaacgcaa
     3301 aattttctgc cccttcgcca atcatcatca catgcgggct ttgctccatc accagccggg
     3361 cggcaagaac cggattacgc agatgactaa cgcccgccac cgcaccggct ttcagggtgt
     3421 taccatccat cacacaggcg tccagttcat gggtttcatc acgcgtaaag acagcgccaa
     3481 ttccggcgtt aaacagtgga cactcttcca gcagacgcac cgcttccgtc accacatcca
     3541 gcgcactttc gccggcttcc agcattttct gcccggtttc aacaatggca gacaacgcct
     3601 cgatgtagcg taattcctgt tgcagactca tctgcgcgcg gctaattgca cctgcgccac
     3661 catgaattgc aatgactgct ttgcccatag tattcgtcca ttaagggtgg ctcgccactt
     3721 ttgctataaa tatcagaatt tttatgaatt actcattcaa tttttgaata tagaaagatg
     3781 gatatggcat gtaaaggcag gagccaacgc tacgcccgag gtggcataca gtctatggcg
     3841 tttttctgac ataataggca aattcgattt tgcctccgca ggagtgtttt catggaattt
     3901 accaccggat tgatgtcgct cgacaccgcg cttaatgaga tgctttctcg cgttacccca
     3961 ctgaccaccc aggaaacgct gccactggtt cagtgttttg gtcgtattct ggcgagcgat
     4021 gtcgtttcgc cactggatgt cccggggttt gataactccg ctatggacgg ctacgcggtg
     4081 cgtttagccg atattgcctc cgggcaaccg ctgcccgttg ccggtaaatc ctttgccggt
     4141 cagccatacc atggtgaatg gcctgcgggt acctgcattc gtattatgac cggtgcgccg
     4201 gtgccggaag gctgcgaagc ggtggtgatg caggagcaga ctgaacaaac ggacaatggc
     4261 gtgcgtttta ctgctgaagt gcgtagcggg caaaatattc gccgtcgcgg tgaagatatc
     4321 tctgcaggtg cggttgtttt cccggcggga actcgcctga ctaccgcaga gctgccagta
     4381 attgcttcac tggggattgc cgaagttccg gtgattcgta aagtgcgtgt agcgcttttt
     4441 tctaccggtg atgaactcca gttgcccggt cagccgctgg gcgacggcca aatctacgac
     4501 accaaccgtc tcgccgtaca cctgatgtta gaacagctgg gttgcgaggt aatcaactta
     4561 gggattatcc gcgacgatcc acatgccctg cgcgccgcat ttattgaagc cgacagccag
     4621 gcggatgtag tgatcagttc cggcggtgtt tcagtgggtg aagcggatta caccaaaacg
     4681 attcttgaag agctggggga gatcgccttc tggaagctgg cgattaaacc aggtaaaccg
     4741 ttcgcgttcg gtaaactcag caatagctgg ttctgcggcc tgccgggcaa cccggtttca
     4801 gcgacgctga ccttctatca actggtacag cctttgctgg caaaactaag cggcaacacc
     4861 gccagcggcc tgcccgcgcg ccagcgcgta cgcacagcgt cccgcctgaa aaaaacgcca
     4921 ggacgtcttg atttccagcg cggcgtgctg caacgcaacg ccgacggtga acttgaagtg
     4981 acgaccaccg gacatcaggg ttcacatata tttagctcct ttagtctcgg caactgcttt
     5041 atcgtgctgg aacgcgatcg cggcaatgtg gacgtgggcg aatgggtgga agtagaaccg
     5101 tttaacgcgt tgttcggagg cctgtaatgg cggaactcag cgatcaggag atgctgcgct
     5161 acaaccggca aatcattctg cgcggttttg attttgacgg tcaggaggcg ctaaaagatt
     5221 ctcgtgtgct ggtagtcggc ctcggtggac tcggctgcgc agcttcgcag tatctggcaa
     5281 gcgccggtgt cggcaacctg acgctgctcg acttcgacac ggtttcgctc tcgaatctgc
     5341 aacgccagac actgcacagt gatgccacgg tcgggcaacc gaaggtggaa tccgcccgtg
     5401 acgccctgac gcggatcaac ccacatatcg cgattacgcc agtcaatgca ctgctagatg
     5461 acgcagaact tgcagcattg attgctgaac acgatctggt gctcgactgt acggataacg
     5521 ttgcggtacg taatcaactg aacgcaggct gttttgccgc gaaggtgccg ctggtttccg
     5581 gcgcggcaat tcgtatggaa gggcaaatca cggtcttcac ttatcaggac ggcgaaccgt
     5641 gctatcgctg ccttagccgt ttgtttggcg agaacgcgtt aacctgcgta gaagcaggcg
     5701 tgatggcacc gttgatcggc gtaattggtt cgttgcaggc aatggaagcg atcaaactgc
     5761 tggcaggtta tggcaaaccg gccagcggga aaatcgtcat gtacgatgcg atgacctgtc
     5821 agtttcgcga aatgaaactg atgcgcaatc cggggtgtga ggtgtgcggg cagtaattgc
     5881 cacctccagg cctgataaga cgcgacaagc gtcgcatcag gcatttgctc cagacgccag
     5941 atgcggcgtg aacgctttat ctggcctacg gttcgggcac agatttccaa gcgcggcagg
     6001 catcggtgca ctccgccacg tcatcagcaa tttatgagga tgcagaacgt ccggttaaat
     6061 cgacgttctg ccaaacgctc cctgccagtc ctgctcaaac ttcgccacag cggcttccac
     6121 cgccggatag ctaatcatct gttgtgccac atccagtggc agagtaattg attcacatcc
     6181 tgccagtaag cagtccagcg cctgacgcgg ggttttgaaa ctcgctgcca gcactttcgc
     6241 ctgcggcgca tgcattttca ataactggtg taagtcggtc acagtctgaa tgccgctacc
     6301 gccctgagca tcaatacgat taacgtaagg cgcaacatat tccgcacctg ccagcgccga
     6361 cagcagccct tgtgccgcgc catataccgc ggttcccagc gtcggaatcc cttccgcttt
     6421 taacatctta atcgctgcca gcccctcggc tgtcaccgga actttcacca cgatatccgc
     6481 aataatagaa cgcagcttaa gcgcgtcatt caccatccct tcagcagtgg tagccattac
     6541 ctgggcaaac agacgcccct gaccgcccat cgcttcatga agttgcggaa gcacaacatc
     6601 cagcggtttt ttacccgcgg cgataatgct tgggttagtg gtcacacccg ccagcggaaa
     6661 aatacgtgac agcgccttca ccgcaacaac gtctgaagta tccagataca gttccataac
     6721 catcctcaaa atgtactttt tgtctgaatg tagactatca cgggaaaact ctctgaacag
     6781 ttgacgcaca tcaatctaac tttcattcga aagtatttta atgtttgaac gaaacagaga
     6841 ggcaactatg attttcaata ttcagcgcta ctcgacccat gatggccccg gtatccgcac
     6901 ggtcgtattt cttaaaggct gttcgctggg ctgccgctgg tgtcagaacc cggaaagccg
     6961 cgcccgtacg caggatctgc tgtatgacgc acgactgtgt ctggaaggct gcgagctgtg
     7021 cgctaaggcc gcgccggaag tgattgagcg cgcgctgaat ggtttgctta ttcatcggga
     7081 aaagttaacc ccggagcatc tgacggcgtt aaccgactgc tgtccgacac aggcgttaac
     7141 cgtgtgtggt gaagtgaaaa gcgttgagga gatcatggcg accgttctgc gcgataaacc
     7201 gttttacgat cgcagcggcg gcggcttaac gctttcgggt ggtgaaccct tcatgcagcc
     7261 ggaaatggcg acggcgctgc tgcaagcctg ccacgaggca ggcattcata ctgcggtaga
     7321 aacctgtctg catgtgccgt ggaaatatat cgccccttct ctgccctata tcgatctgtt
     7381 tcttgccgat ttaaaacacg ttgccgacgc gccgtttaaa cagtggaccg acggtaacgc
     7441 cgccagggtg ctggataacc tgaaaaaact ccccgcagcg ggcaaaaaaa tcattatccg
     7501 cgtgccgctg attcagggct ttaatgccga cgaaacctct gtaaaagcca ttaccgattt
     7561 tgccgccgac gagctgcacg ttggcgaaat tcattttctg ccctaccaca cgctgggcat
     7621 caacaaatat cacttactta atctgcccta tgacgccccg gaaaaaccgc ttgatgcgcc
     7681 agagctgctc gattttgccc agcagtatgc ctgccagaaa gggttaaccg cgaccttacg
     7741 aggataacaa ccatgaccac actgaaactg gacacgctca gcgaccgcat taaagcgcac
     7801 aaaaatgcgc tggtgcatat tgtgaaaccg ccagtctgta ccgagcgcgc gcagcactat
     7861 accgagatgt atcaacaaca tctcgataag ccgatcccgg tacgtcgcgc gctggcactg
     7921 gcgcatcacc tggcgaatcg caccatctgg atcaaacacg atgagttgat cattggcaac
     7981 caggcaagcg aagttcgcgc cgcgccgatc ttcccggaat atactgtctc gtggatcgaa
     8041 aaagagattg atgatctggc agatcgtccc ggtgctggct ttgcggtgag cgaagagaac
     8101 aaacgtgttc tgcatgaagt gtgcccgtgg tggcgcggtc agacggtaca ggatcgctgc
     8161 tatggcatgt ttaccgatga gcaaaaaggt ctgctggcga ccggcatcat taaagcggaa
     8221 ggcaatatga cctccggcga tgcgcacctg gcggtcaatt tcccgctgct gctggaaaaa
     8281 gggcttgatg gtctgcgtga gaaagtagcg gaacgtcgct cgcgcatcaa tctgacggtg
     8341 ctggaagatt tgcacggcga acagttcctg aaagcgattg atatcgtgct ggtggcagtc
     8401 agtgaacaca ttgaacgttt cgctgccctg gcgcgtgcaa tggccgcgac cgaaacccgc
     8461 gaaagccgtc gcgatgaact gctggcgatg gcagaaaact gcgatcttat cgcccaccag
     8521 ccgccgcaga ctttctggca ggcgctgcaa ctgtgttact tcatccagtt gattttgcag
     8581 attgaatcta acggtcactc ggtgtcgttt ggtcgtatgg atcagtatct ctacccgtac
     8641 tatcgccgcg acgttgaact gaaccagacg ctggatcgcg aacacgccat cgaattgctg
     8701 catagctgct ggttgaaatt gctggaagtg aacaaaatcc gctccggctc ccactcaaaa
     8761 gcctctgcgg gaagtccgct gtatcagaac gtcaccattg gcggacaaaa tctggttgat
     8821 ggtcaaccaa tggacgcggt gaatccactc tcttacgcga tcctcgaatc ctgcggtcgc
     8881 ctgcgttcca ctcagcctaa cctcagcgtg cgttaccatg caggaatgag taacgatttc
     8941 ctcgacgcct gcgtacaggt gatccgctgc ggcttcggga tgccggcatt caacaacgac
     9001 gaaatcgtga tcccggaatt tattaaactc ggtattgaac cgcaggacgc ttatgactac
     9061 gcagcgattg gttgtataga aaccgccgtc ggtggcaaat ggggctatcg ctgtaccggc
     9121 atgagcttta tcaacttcgc ccgcgtgatg ctggcggcgc tggaaggcgg gcgtgatgcc
     9181 accagcggca aagtgttcct gccacaagaa aaagcgttgt cggcaggtaa cttcaacaac
     9241 ttcgatgaag tgatggacgc gtgggatacg caaatccgtt actacacgcg taaatcaatc
     9301 gaaatcgaat atgtcgtcga caccatgctg gaagagaacg tgcacgatat tctctgctcg
     9361 gcgctggtgg atgactgtat tgagcgagcg aaaagtatca agcaaggcgg cgcgaaatat
     9421 gactgggttt ctggcctgca ggtcggtatt gccaacctcg gcaacagcct ggcggcagtg
     9481 aagaaactgg tgtttgaaca gggcgcgatt ggtcagcaac agctggctgc cgcactggcg
     9541 gatgacttcg atggcctgac tcatgagcaa ctgcgtcagc ggctgattaa cggtgcgccg
     9601 aagtacggca acgacgatga taccgtcgat acgctgctgg ctcgcgctta tcagacctat
     9661 atcgacgaac tgaaacagta ccataaccca cgctacggtc gtggtccggt tggcggcaac
     9721 tattacgcgg gtacgtcgtc tatctccgct aacgtaccgt ttggcgcgca aactatggca
     9781 acgccggacg ggcgtaaagc ccacaccccg ctggcagaag gcgctagtcc ggcctctggt
     9841 actgaccatc tcggccctac tgcggtcatt ggctcagtgg gtaaactgcc tacggcagcg
     9901 attctcggcg gcgtgttgct caaccagaaa ctgaatccgg caacgctgga gaacgaatct
     9961 gacaagcaga aactgatgat cctgctgcgt accttctttg aagtgcataa aggctggcat
    10021 attcagtaca acatcgtttc ccgcgaaacg ctgctggagg cgaaaaaaca tccggatcag
    10081 taccgcgatc tggtagtacg tgtcgcgggt tattccgcct tcttcaccgc gctctctcca
    10141 gacgctcagg acgatatcat cgcccgtact gaacatatgc tgtaatctct tcggcccggc
    10201 tgcggcgtcg ccgggctaaa tcattcacat catcaatttc atccttactt tcattcgaaa
    10261 tataatttgt gctctgcgtc acattgttat tacactgtta cggtttcatt ttcgagccag
    10321 gagcacacct atgagcgtaa aagttatcgt cacagatatg gacggtactt ttcttaacga
    10381 cgccaaaacg tacaaccaac cacgttttat ggcgcaatat caggaactga aaaagcgcgg
    10441 cattaagttc gttgtcgcca gcggtaatca gtattaccag cttatttcgt tctttcctga
    10501 gctaaaggat gagatctctt ttgtcgcgga aaacggcgca ctggtttacg aacatggcaa
    10561 gcaactgttc cacggcgaac tgacccgaca tgaatcgcgg attgttattg gcgagttgct
    10621 aaaagataag caactcaatt ttgtcgcctg cggtctgcaa agtgcatatg tcagcgaaaa
    10681 tgcccccgaa gcatttgtcg cactgatggc aaaacactac catcgcctga aacctgtaaa
    10741 agattatcag gagattgacg acgtactgtt caagttttcg ctcaacctgc cggatgaaca
    10801 aatcccgtta gtgatcgaca aactacacat agcgctggat ggcattatga aacccgtcac
    10861 cagtggtttt ggctttatcg acctgattat tcccggtcta cataaagcaa acggtatttc
    10921 gcggttactg aaacgctggg atctgtcacc gcaaaatgtg gtagcgattg gcgacagcgg
    10981 taacgatgcg gagatgctga aaatggcgcg ttattccttt gcgatgggca atgctgcgga
    11041 aaacattaaa caaatcgccc gttacgctac cgatgataat aatcatgaag gcgcgctgaa
    11101 tgtgattcag gctgtgctgg ataacacatc cccttttaac agctgacctc tctaccggag
    11161 ccatgcgctc cggttttccc ttctttttac atcctgtaca tcacatcaaa tcattaaact
    11221 tgcattaact tatttataac ttgtattgtc acatgcaaat cagcatagtt tcgaatgaaa
    11281 gttaatgcga ggtggttatg gcttctactt ttaccagcga cacattgcct gccgatcaca
    11341 aagcagctat ccgtcagatg aagcacgcgc tgcgggcgca gcttggcgat gtccagcaga
    11401 tctttaatca gctaagcgat gacattgcca cgcgagtggc tgaaatcaac gcactcaaag
    11461 cacagggcga tgccgtctgg ccggtgctgt cttatgccga tatcaaagca ggtcatgtta
    11521 ctgcagagca gcgcgaacag attaaacgtc gcggttgtgc ggtgataaaa ggccatttcc
    11581 cccgcgaaca agcgctaggc tgggatcagt cgatgctgga ctatctggac cgcaaccgct
    11641 ttgacgaggt ctacaaaggc cccggcgata atttcttcgg gacgctcagc gcttcacgtc
    11701 ccgagattta ccccatctac tggtcgcagg cgcaaatgca ggcccgccag agtgaagaaa
    11761 tggcgaatgc gcagtcgttt ctcaatcgtc tgtggacatt tgaaagtgat ggaaagcaat
    11821 ggtttaaccc ggatgtgagc gtcatctacc ctaaccgtat ccgccgcctt ccgcccggaa
    11881 cgacctccaa aggtcttgga gcgcataccg actccggggc gctggaacgc tggctgcttc
    11941 cagcatatca gcacgttttc gccaacgtct ttaatggcaa tctggcgaaa tacgatccct
    12001 ggcatgcggc acatcgtacg gaagttgaag agtacacggt ggacaacact accaaatgtt
    12061 ccgtgtttcg gacattccag ggctggactg cgctctcgga tatgctgcct ggtcaggggt
    12121 tgctgcacgt cgtgcccatt cctgaagcca tggcgtacgt actgttacgt ccgctgcttg
    12181 atgatgtgcc ggaggatgaa ctgtgcggcg tagcgcccgg aagagtgttg ccggtatcag
    12241 agcaatggca tccactgtta attgaggcgt taaccagcat tccaaaactc gaggccggag
    12301 actccgtctg gtggcactgc gacgtcatcc attccgttgc ccccgttgaa aatcaacagg
    12361 gttggggcaa cgtgatgtac attcctgcgg caccgatgtg cgagaaaaat cttgcctacg
    12421 cgcacaaggt gaaggccgca ctggaaaaag gcgcatcgcc gggcgacttc ccgcgcgagg
    12481 actatgaaac aaactgggaa ggacgcttta cgcttgccga cctcaacatt cacggtaagc
    12541 gagcgttggg catggatgtt tgagagttaa tggaagtgaa ttgatgtggg attaagaaaa
    12601 tcgattcgac gttactcaga ttgctgacaa gtgcgctttc ttaatgcctg atgcggcgtt
    12661 aacgccttat ccggcctaca aagtcctgca gattcaatat attacaggta ctgcgtaggc
    12721 ctgataagcg tagcgcatca ggctattttg catttgtcat cagccgtcgt caccccgaca
    12781 gtatcggggt gacggataat cacttactcg atccctttac tacgcaggta atcttcgtaa
    12841 ttaccgctaa agtcgatcac gcgttccggg gtgatttcca gaatgcgggt cgccagggag
    12901 cttacgaact cacggtcgtg tgaaacaaag atcagcgtgc cctgatacag ttccagcgcc
    12961 atgttcagtg actcgatgga ttccatatcc aggtggttgg tcggttcgtc catgatcaga
    13021 atgttcggct tctgcatcat taacttacca aacagcatcc gccctttttc cccaccggaa
    13081 agcactttag ctggcttttt gatgtcgtcc tggctgaaca gcaaacgacc gagaatgctg
    13141 cgtaccgcct gctcgtcatc gccttcctgc ttccactgac tcatccattc gaacacggtc
    13201 agatcatttt caaactcata ttcgtggtcc tgggcgtagt aaccaatgcg cgcgttttca
    13261 gaccatttta cggtgccgct gtccggttgc agatcgccca ccagcgtttt cagcagcgtt
    13321 gatttaccga cgccgttggt acccagtacc gcaagttttt caccgacctc cagcagcagg
    13381 ttgagatttt taaacagtgg accgttatca aaccctttgg tcagaccttc cacttccagc
    13441 gcgttacgga acagtttctt atcctgttca aaacggatga acgggttctg acggctggag
    13501 gctttcacct cttccagttt gattttatca atctggcgcg cgcgcgaagt tgcctggcga
    13561 gatttcgagg cgttggcgct aaagcggcta acgaaagatt gcaactcagc aatctgcgct
    13621 ttcttcttgg cgttatcggc cagcagacgt tcacgcgcct gggtcgccgc cgtcatgtac
    13681 tcatcgtagt tacccggata aacgcgcagc tcgccgtaat ccagatccgc catgtgggta
    13741 cagaccatgt taaggaagtg acggtcgtgc gagatgatga tcatggtgct gtcacgctcg
    13801 ttcagcacct gttccagcca gcgaatggtg tcgatgtcga ggttgttggt cggttcgtcg
    13861 agcaggagaa tatccggctc agcaaacagc gcctgcgcca gaagcacacg cagcttccag
    13921 ccaggagcaa cttcactcat cgggccgtag tgctgttcca ctggaattcc cacgccaagc
    13981 aacagttcac cggcgcgagc ttccgcagag taaccgtcca tttcgccgta tttcacttcc
    14041 agatcggcca ctttatagcc gtcttcttca ctcatttccg gcaaagcata gatgcggtcg
    14101 cgctcctgct tcacttccca caactcttta tgccccatga tcaccgtatc cagcacagtg
    14161 aactcttcaa aggcaaactg atcctgacgc agcttaccaa tgcgctcgtt gggatcgagg
    14221 gaaacgttac ccagcgtcgg ctcaaggtcg ccgccgagaa tcttcataaa ggtggattta
    14281 ccactaccgt tcgcgccaat caggccgtaa cggttgccgc cgccgaattt gacggaaatg
    14341 ttttcaaata acggcttact gccgaactgc atggtgacgt tactggaaac taacacaggc
    14401 gtatcctgaa aagagatatg acaaaccgcg cattatgcca taagtctgaa agaagatcct
    14461 gctcctcgct taaggtctac actcaagcca aaggcaaaaa atgtgatttc gtacacatct
    14521 gatttcactg tgagctggaa tgaacttata atgcgcttcc aatactctaa tattctcaac
    14581 ccaatggcct gccaggcaca aaatctcgct taacatgaat atgaaattga aaacattatt
    14641 cgcagcggcc ttcgctgttg tcggcttttg cagtaccgcc tctgcggtaa cttatcctct
    14701 gccaaccgac gggagtcgcc tggttggtca gaatcaggtg atcaccattc ctgaaggtaa
    14761 cactcagccg ctggagtatt ttgccgcgga gtaccagatg gggctttcca atatgatgga
    14821 agcgaacccg ggtgtggata ccttcctgcc gaaaggcggt actgtactga acattccgca
    14881 gcagctgatc ctgccggata ccgttcatga aggcatcgtc attaacagtg cagagatgcg
    14941 tctgtattac tatccgaaag ggaccaacac cgttatcgtg ctgccgatcg gcattggtca
    15001 gttaggcaaa gatacgccta tcaactggac caccaaagtt gagcataaga aagcaggccc
    15061 gacctggacg ccgaccgcca aaatgcacgc agagtaccgc gctgcgggcg aaccgctacc
    15121 ggctgtcgtt ccggcaggtc cggataaccc gatgggtctg tatgcactct acatcggtcg
    15181 cctgtatgct atccatggca ccaacgccaa cttcggtatc ggcctgcgtg taagtcatgg
    15241 ttgtgtgcgt ctgcgtaacg aagacatcaa attcctgttc gagaaagtac cggtcggtac
    15301 ccgcgtacag tttattgatg agccggtaaa agcgaccacc gagccagacg gcagccgtta
    15361 tattgaagtc cataacccgc tgtctaccac cgaagcccag tttgaaggtc aggaaatcgt
    15421 gccaattacc ctgacgaaga gcgtgcagac agtgaccggt cagccagatg ttgacctggt
    15481 tgttcttgat gaagcgatca aaaatcgttc cgggatgccg gttcgcctga attaatcgtc
    15541 aaaacgtaaa acaaaggcgg actcataatc cgcctttttt atttgccaga ccttaccagg
    15601 ccgggatcat aactaacaaa acgtatccca ccagcgccgc ccacaacagc atcggtattg
    15661 aatagagatg gaaacgccac cagatgcggc gatcgttcgc catacgtagc gcaatcaaat
    15721 ttgccagcga tcctggtaat aaaccaaagc cccccacgtt taccgcccat gccagtaata
    15781 aagacggagg tacataattg agcagcaaaa tggtactcgg cacgttactg atcacctgcg
    15841 ataaaccgac tgccgttaac cataacccgg gttcagataa atgactcacg ttacccaaca
    15901 cgccttgcaa cgctggaagc tgggtcagta aatggacgtc gataaacatc gccataaaca
    15961 ccagcagcaa cgtccagtcc acactcaaca ccacgcgacg cgccagcagc acaaagccca
    16021 ccgccacaat caccagtccc cacagctctt gtttgaactc cagcgccgtc agaaagacga
    16081 tatacagccc cagacaattc cacaccagcc gcggtttcca ctccggtgtt tgcacccccg
    16141 tatggtattg cagcgccttt ccagggaaac aacaccagca caacagcagg agtgtcagca
    16201 tcattgcgcc agccagcggt gccatttggg caataaatcc ggcaaacgaa agaccagaac
    16261 gtccccagat aagaatattt tgcgggttgc caattggcgt cagtagcgaa ccagcgttga
    16321 ctgccagcgc ctcaaaaata atcagccgat taaccgggat ctcacacagt ctttttagcg
    16381 tgatagtcag cggaacaaca ataaacagcg cgacatcgtt ggtcagaaag gtagaaagca
    16441 gcgccgccgc cagcaccata aacatcgcca gccgacgctc cgtagcaaag cggcgcacca
    16501 ttttgcgccc cagcacatca aaataaccgc ttaactccac acctttggtc agcagcatca
    16561 ggccgcttaa ggtgatgatg gtgtgccagt cgatagcagc aggccaggat ttcggtgcaa
    16621 agggcacgaa aaagcttaat ccgataccaa caagaattaa taactgaaaa aaacgatcgc
    16681 cttgcagcgt gcgtaaaaaa ggcaggctca tttggcaccg tgtttctggg taaacaaacg
    16741 aaaagcgtcg agcgtctctt cactaacatg gtgctccatg ccttccgcgt cgcgacgggc
    16801 gatttccgga ctgacgccca acaccaacaa gaaattttcg actatctgat gtcgctcgcg
    16861 gctttcctgc gccagcttct ctccttctgc cgttaaaaac acgccacgcc aggggatcat
    16921 ttcaatcagc cccatggttg ccagccgctt aagcatttta gccaccgtcg gttgcgaaac
    16981 tcccagacga gcagccatgt ccacctgacg agcttccccc acttccctga tcaagtcaga
    17041 aatcagctca acgtaatcat caataagctc gcgccgatgc gcctctctga cctggcggaa
    17101 cccttcaacg tgctcttcca cgttcactaa ctgcgtcact ttttttgctg ttggcgtacc
    17161 tgcgcgacga ctcattgtgc ttcctcatta tggtgacgca tacatagcgt tctcatttcg
    17221 tgcgcacatt gtaaaccaga gttgcgaagg tacaaaaaat taacgtttta gcaatagcta
    17281 tataatatag cctgtgctat atctgtatgt aatgcaatca tccctcaagg atcgacggga
    17341 ttagcaagtc aggaggtctt atgaatgagt tcaagaggtg tatgcgcgtg tttagtcatt
    17401 ctccctttaa agtacggtta atgctgctct ctatgttgtg cgatatggtc aacaacaaac
    17461 cgcagcaaga taaaccttca gataaatagc ggcgtcgcgg tacgccgctt cactcctgct
    17521 tttatgcagg cataacgcgt tttggtctga aaaaacccac tttttgtcgg atttgcaatc
    17581 cccttcgcaa aagatttgtt cgtcagtagt tgacctgaac ggcggctcgc tctatcttct
    17641 tgcagccctg cgtatattgc ggctcgcgga tgcggacccc tttcccctct tcacgcactc
    17701 ttgcaggtat tgacccttga cgccagggta agcacatggc gtttgttacg atagtggcat
    17761 atgaatttaa ccctcaaaga atcgcttgtt acccgtagcc gggtatttag cccgtggact
    17821 gcgttctact ttttacagtc gctattaatt aacctcggct taggttaccc cttcagtttg
    17881 ctctacaccg ctgcgtttac ggctattttg cttttgctat ggcgaacatt gcctcgcgta
    17941 caaaaagttc tggtcggtgt cagttcgctg gtggcggctt gttatttccc ttttgctcag
    18001 gcctacggcg cgcctaattt caatacattg ctggcattgc actccaccaa tatggaagag
    18061 tcgaccgaaa tcctgacgat ttttccgtgg tacagctacc tggtcggctt atttattttt
    18121 gcgctcggcg taatagcaat caggcgaaaa aaagagaatg aaaaagcgcg ctggaatacc
    18181 ttcgacagcc tgtgtctggt attcagtgtg gcgacatttt ttgttgctcc cgtgcaaaac
    18241 ctggcctggg gtggcgtatt taaactgaaa gatactggct atccggtatt tcgttttgct
    18301 aaggatgtca tcgtcaataa taacgaggtg attgaagagc aagaacggat ggcaaaactt
    18361 tccggaatga aagatacttg gacggtcact gccgttaagc cgaagtatca gacctatgtg
    18421 gtagtgatcg gtgaaagcgc gcgtcgcgat gccctcggtg cctttggcgg tcactgggac
    18481 aataccccgt ttgccagcag cgttaacggt ttgatatttg ctgactacat tgccgccagt
    18541 ggctccacgc agaaatcgct tggcttaacg ctcaatcgcg tagtcgatgg caaaccacag
    18601 tttcaggata actttgtcac cctggcaaat cgcgcgggct tccagacctg gtggttttcc
    18661 aaccagggcc aaatcggcga atacgatact gctatcgcca gcatcgccaa acgtgcagat
    18721 gaagtgtact tcctgaaaga aggtaatttt gaagcagata aaaacacgaa agacgaagcg
    18781 ttactggata tgaccgctca agtgctggcg caagagcact cgcaaccgca gctgattgtt
    18841 ctgcatctga tgggctcgca tccgcaggcc tgcgacagga cacaaggcaa atacgaaacc
    18901 tcctttgtgc aatcgaaaga aacgtcgtgc tatctctata ccatgacgca aacggacgat
    18961 ttactgcgca agctgtacga tcagttacgc aacagcggca gcagcttctc tctggtttac
    19021 ttttctgacc acggtctggc ctttaaagag cgcggcaaag acgtgcaata ccttgcccat
    19081 gatgataaat atcagcaaaa tttccaggtg ccttttatgg tcatttccag cggcgataaa
    19141 gcgcatcggg tgattaaagc ccgccgctca gccaatgact tcttaggctt tttctcgcag
    19201 tggacgggaa ttaaagcgaa ggaaataaat atcaaatacc cgtttatatc tgagaagaaa
    19261 gccgggccga tatacatcac caacttccag ttacagaagg tggattacaa ccatctcgga
    19321 accgatattt tcgacccgaa accttaaaaa caaaaatccg ccccgagagg cggattttta
    19381 tatcaccaaa gtgattagaa gcggtaacca acaccggcaa tccaggtgcc tacgtcaacg
    19441 ctacgaatac ggctctgctc gtaagagaag tccagagcaa cgttttccat cgggttgaac
    19501 tgcagaccag caccgtagga gaaaccgtag tcgctggtgt cgtgtttgta ggtcgggtat
    19561 tcagtggtct ggaatttacc ataacccaca cccactacac cgtagatgct tgcccagtcg
    19621 ttaatgcggt aagccggacc agcagtgatg ccatagtact ggtttttgtt gtagtcacca
    19681 gagcttgcag tacggctttt ctcggtgtaa gtgaaagaac cgatcacacc cagcgggctg
    19741 ttgtcttctt catagcggta tttcaggttg aaaccgccca ttttgttcat ttggccctga
    19801 gcgtcgctct gtgcgtaacc gccagttacg gtagaagtcg cagctacgga agtacctgcg
    19861 gtgaaagcca gaactgcggc cagtgctgaa agacatgcaa tttttttcat aaccacctca
    19921 aatgtgattc aaataagtcc taagttttaa atatatcaaa aattaatggg aaactcttcg
    19981 cgatttgtga tgtctaacgg gccatttcat gtaacagaac gtttccatac accgctatcc
    20041 atctaaattt aaatcacttt ttcagagaac tgcgtaagta ttacgcatgt tttccctgtc
    20101 attcatccag attattccta atcaccagac taatgattcc atcaatcctg gcgcatttta
    20161 gtcaaaacgg gggaaaattt tttcaacaaa tgctcaacca gcattgggta tatccagtac
    20221 actccacgct ttacttaagt ctagatattt gtgggagaaa ggatgcctgg ttcattacgt
    20281 aaaatgccgg tctggttacc aatagtcata ttgctcgttg ccatggcgtc tattcagggt
    20341 ggagcctcgt tagctaagtc actttttcct ctggtgggcg caccgggtgt cactgcgctg
    20401 cgtctggcat taggaacgct gatcctcatc gcgttcttta agccatggcg actgcgcttt
    20461 gccaaagagc aacggttacc gctgttgttt tacggcgttt cgctgggtgg gatgaattat
    20521 cttttttatc tttctattca gacagtaccg ctgggtattg cggtggcgct ggagttcacc
    20581 ggaccactgg cggtggcgct gttctcttct cgtcgcccgg tagatttcgt ctgggttgtg
    20641 ctggcggttc ttggtctgtg gttcctgcta ccgctggggc aagacgtttc ccatgtcgat
    20701 ttaaccggct gtgcgctggc actgggggcc ggggcttgtt gggctattta cattttaagt
    20761 gggcaacgcg caggagcgga acatggccct gcgacggtgg caattggttc gttgattgca
    20821 gcgttaattt tcgtgccaat tggagcgctt caggctggtg aagcactctg gcactggtcg
    20881 gttattccat tgggtctggc tgtcgctatt ctctcgaccg ctctgcctta ttcgctggaa
    20941 atgattgccc tcacccgttt gccaacacgg acatttggta cgctgatgag catggaaccg
    21001 gcgctggctg ccgtttccgg gatgattttc ctcggagaaa cactgacacc catacagctg
    21061 ctggcgctcg gcgctatcat cgccgcttca atggggtcta cgctgacagt acgcaaagag
    21121 agcaaaataa aagaattaga cattaattaa atttacattt ctgtatggtt atgcataacc
    21181 atgcagaatt tctcgctact tttcctctac accgtcttta tatatcgaat tatgcaaaag
    21241 catatttatt ccgaaaattc ctggcgagca gataaataag aattgttctt atcaatatat
    21301 ctaactcatt gaatctttat tagttttgtt tttcacgctt gttaccacta ttagtgtgat
    21361 aggaacagcc agaatagcgg aacacatagc cggtgctata cttagtctcg ttaattactg
    21421 ggacataaca tcaagaggat atgaaattat gagtaccgct aaattagtta aatcaaaagc
    21481 gaccaatctg ctttataccc gcaacgatgt ctccgacagc gagaaaaaag caacagtaga
    21541 gttgctgaat cgccaagtta tccagtttat tgatctttct ttgattacca aacaagcgca
    21601 ctggaacatg cgcggcgcta acttcattgc cgtacatgaa atgctggatg gcttccgcac
    21661 cgcactgatc gatcatctgg ataccatggc agaacgtgcc gtgcagctgg gcggtgtagc
    21721 tctggggacc actcaagtta tcaacagcaa aaccccgctg aaaagttacc cgctggacat
    21781 ccacaacgtt caggatcacc tgaaagaact ggctgaccgt tacgcaatcg tcgctaatga
    21841 cgtacgcaaa gcgattggcg aagcgaaaga tgacgacacc gcagatatcc tgaccgccgc
    21901 gtctcgcgac ctggataaat tcctgtggtt tatcgagtct aacatcgaat aaatccatcg
    21961 ctgatggtgc agaactttag tacccgataa aagcggcttc ctgacaggag gccgttttgt
    22021 tttgcagccc acctcaacgc acttatttag tgcatccatc tgctatctcc agctgattaa
    22081 gtaaattttt tgtatccaca tcatcacaca atcgttacat aaagattgtt ttttcatcag
    22141 gttttacgct aaataatcac tgtgttgagt gcacaatttt agcgcaccag attggtgccc
    22201 cagaatggtg catcttcagg gtattgccct ataaatcgtg catcacgttt ttgccgcatc
    22261 tcgaaaaatc aatgagttgc aaaactggca cgattttttc atatatgtga atgtcacgca
    22321 ggggatcgtc ccgtggatag aaaaaaggaa atgctatgaa gtctgtatta aaagtttcac
    22381 tggctgcact gaccctggct tttgcggttt cttctcatgc cgcggataaa aaattagttg
    22441 tcgcgacgga taccgccttc gttccgtttg aatttaaaca gggcgataaa tatgtgggct
    22501 ttgacgttga tctgtgggct gccatcgcta aagagctgaa gctggattac gaactgaagc
    22561 cgatggattt cagtgggatc attccggcac tgcaaaccaa aaacgtcgat ctggcgctgg
    22621 cgggcattac catcaccgac gagcgtaaaa aagcgatcga tttctctgac ggctactaca
    22681 aaagcggcct gttagtgatg gtgaaagcta acaataacga tgtgaaaagc gtgaaagatc
    22741 tcgacgggaa agtggttgct gtgaagagcg gtactggctc cgttgattac gcgaaagcaa
    22801 acatcaaaac taaagatctg cgtcagttcc cgaacatcga taacgcctat atggaactgg
    22861 gcaccaaccg cgcagacgcc gttctgcacg atacgccaaa cattctgtac ttcatcaaaa
    22921 ccgccggtaa cggtcagttc aaagcggtag gtgactctct ggaagcgcag caatacggta
    22981 ttgcgttccc gaaaggtagc gacgagctgc gtgacaaagt caacggcgcg ttgaaaaccc
    23041 tgcgcgagaa cggaacttac aacgaaatct acaaaaaatg gttcggtact gaaccgaaat
    23101 aataacgcta cacctgtaaa gcgcactggc agttccctct cccccctggg gagaggatta
    23161 gggtgagggg cgcaaacccg ctccggggcc attaattacc ctgaatttga ttatttacac
    23221 cacggtaaca ggaacaacat atgcagtttg actggagtgc catctggcct gccattccgc
    23281 ttctgattga aggtgccaaa atgaccctgt ggatttcggt cctcggtctg gctggcggtc
    23341 tggtaatcgg attgctggca ggttttgcac gcaccttcgg tggttggata gccaaccacg
    23401 tcgcgctggt ctttattgaa gtgatccgcg gcacacctat cgtcgtccag gtgatgttta
    23461 tctatttcgc cctgccgatg gcgtttaacg acttacgcat cgacccattt actgcggcgg
    23521 tggtcaccat catgatcaac tccggcgcgt acattgcgga aatcacgcgt ggtgcggtgc
    23581 tgtctatcca caaaggtttt cgtgaagcag gactggcgct cggtctttca cgttgggaaa
    23641 ccattcgcta cgtcatttta ccgctggcac tgcgtcgtat gctgccgccg ctgggtaacc
    23701 agtggatcat cagcattaaa gacacctcgc tgtttattgt tatcggcgtg gcggaactga
    23761 cccgtcaggg gcaagaaatt attgccggta acttccgcgc ccttgagatc tggagcgccg
    23821 tggcggtgtt ctatctgatt attaccctgg tgctgagctt tattctgcgt cgtctggaaa
    23881 gaaggatgaa aatcctgtga ttgaatttaa aaacgtctcc aagcactttg gcccaaccca
    23941 ggtgctgcac aatatcgatt tgaacattgc ccagggcgaa gtcgtggtga ttatcgggcc
    24001 gtccggttcc ggtaaatcga ccctgctgcg ctgcatcaac aaactggaag aaatcacctc
    24061 cggcgatctg attgtcgatg gcctgaaggt taacgatccg aaagttgacg agcgcctgat
    24121 tcgccaggaa gcaggtatgg tgttccagca gttttacctc ttcccgcatc tgacggcgct
    24181 ggaaaacgtc atgtttggtc cgctacgcgt gcgtggcgcg aacaaagaag aggcggaaaa
    24241 actggcgcgt gagctgctgg cgaaagtcgg tctggcagaa cgtgcacatc actacccttc
    24301 cgaactttct ggtggtcaac agcagcgtgt ggcgattgcc cgcgcgctgg cggtgaagcc
    24361 gaaaatgatg ctgtttgatg aaccgacttc cgctcttgac ccggaactgc gccatgaagt
    24421 gctgaaggtt atacaggatc tggctgaaga agggatgacg atggtgatcg tgacccacga
    24481 aatcggtttt gccgagaaag tagcttcgcg gctgatcttt atcgacaaag gccggattgc
    24541 ggaagatggc aatccgcagg tgttgatcaa gaacccgccg agccagcgct tgcaggaatt
    24601 tttgcagcac gtctcttaat aagacacatt gcctgatgcg ctacgcttat caggcctaca
    24661 ggatatctgg caacttatta aaattgcatg aacttgtagg acggataagg cgttcacgcc
    24721 gcatccggca aaaaagcccg cacgttgtca gcaacctgct taatatccct tcctcccttt
    24781 cacccgaaag ggaggcacac cagattcctc tcatttaaaa tcgcccctcc cccagcatct
    24841 atacttatct ttttgctctg ttttctcact ggaggagtca tgcggtggat cctgttcatc
    24901 ctcttctgcc tgctgggcgc acctgcccac gcggtatcca tacccggcgt tacaaccaca
    24961 acgacaacgg actcaacgac tgaaccggcc ccggaaccgg atatcgaaca aaaaaaagcg
    25021 gcctatggcg cactggcgga tgtgctggat aacgacacct cgcgtaaaga gttgatcgac
    25081 cagttgcgta ctgtcgccgc gacgccccct gcggaaccgg tcccgaagat cgtgccgccg
    25141 acgctggttg aagagcaaac cgtgctgcaa aaggtcaccg aagtcagccg ccattatggt
    25201 gaagcccttt ccgcccgctt cgggcaactt tatcgcaata tcaccggctc cccgcataag
    25261 ccatttaatc cacaaacctt cagcaatgcg ctgagccatt tttcaatgtt agcggtatta
    25321 gtgtttggtt tttactggct gattcgcctg tgcgcactgc cgctgtatcg caaaatgggt
    25381 cagtgggcga ggcaaaaaaa tcgtgagcgc agcaactggt tgcagcttcc ggcgatgatt
    25441 attggggcgt ttattatcga cctgctgtta ctggccctga cattatttgt cggccaagta
    25501 ttaagcgaca acctgaatgc aggcagtcgc accatcgctt tccaacaaag tttgtttctc
    25561 aacgcctttg ccctgattga atttttcaaa gccgtactac gcctgatttt ttgcccaaac
    25621 gtggcggagc tgcgcccgtt cacgattcag gacgagagcg cccgttactg gagtcgtcgc
    25681 ctgagctggt taagcagcct gattggctat ggcctgattg tggccgtgcc gattatctct
    25741 aatcaggtga atgtacagat aggtgcgctg gcgaacgtca tcattatgct gtgcatgacc
    25801 gtctgggcgt tgtacctgat cttccgaaat aaaaaagaga ttacccagca tttgctcaac
    25861 tttgcggagc attcgctggc ttttttcagc ctgtttatcc gcgcctttgc gctggtgtgg
    25921 cactggctgg caagcgccta ttttatcgtg ctgtttttct tttcgttgtt cgatccgggc
    25981 aacagtctga aatttatgat gggtgcaacg gtgcgcagcc tggcgattat tggtatcgca
    26041 gcgtttgttt ccggtatgtt ttcccgctgg ctggcgaaaa ccatcaccct ctcgccacat
    26101 actcagcgta actatccgga gctacaaaaa cggctgaatg gctggctttc ggcggcgctg
    26161 aaaacggcgc gtattctgac cgtctgcgtg gcagtaatgt tgctgttgag cgcatgggga
    26221 ttgttcgatt tctggaactg gctgcaaaac ggcgcggggc agaaaaccgt agatatcctg
    26281 atccgcatcg cactcattct tttcttctcg gcggttggct ggacggtgct cgccagtttg
    26341 atcgaaaacc ggctggcttc ggatattcat ggccgcccgc tacccagcgc ccgtacgcgt
    26401 accctgctga cgctgttccg taacgcgctg gcggtgatta tcagtaccat caccatcatg
    26461 attgtgttgt cggaaatcgg cgtcaatatc gcgccattgc tggcaggtgc cggggcatta
    26521 ggtctggcta tctcgtttgg ttcgcaaacg ctggtaaaag atattatcac cggggtattt
    26581 attcagtttg aaaacggcat gaacactgga gatttggtga ctatcgggcc gttgaccggc
    26641 acagtggaac ggatgtcgat tcgctccgtg ggcgtgcgcc aggataccgg ggcgtatcac
    26701 atcattccgt ggtcttcgat aaccaccttt gctaacttcg tccgcggtat tggttcggta
    26761 gtggcaaact acgatgttga tcgccatgaa gatgctgata aagccaatca ggcactgaaa
    26821 gatgcggtaa cggaattgat ggaaaacgaa gaaattcgcg ggctgattat tggtgaaccg
    26881 aactttgccg ggattgtcgg cttaagcaat accgcgttta cactgcgtgt ttcgttcacc
    26941 acgctgccac tcaaacagtg gacggtacgc tttgccctcg acagccaggt gaaaaaacat
    27001 ttcgacctgg cgggcgttcg cgtgccagtg cagacttatc aggtgctgcc tgctccgggc
    27061 gctcccccgg ctgaaccgct gccgccgggg gagccaacgc tttaacgttg gcgattaaca
    27121 aaacggcggc gctgctcgtc gttcataaag gtccaggcaa taaagcgact ctgcttttgc
    27181 ccctgggcca tctctttttt taccaccttc accgcgccca cgtcggtcag agcacgatac
    27241 aacggcggta agttttcacc acgagatacc agcgaggtaa accacatcac ctgcttcgcg
    27301 aagcctttgc tctcttcaat catctttttg ataaagttga cttcaccgcc ttcacaccac
    27361 aactcctgtt gctgaccacc aaagttcagt gcatcgtctt tgttcagccc caggttacgg
    27421 cgtttacgct cactacctgc ccgcgccgca gcggcggaat cgtggaatgg cgggttacac
    27481 aaggtcgcgt cgtattgctc gtttttgtgg atgatgccgt taaaaatcgc cccactctct
    27541 ttttgccgac gcagacgaat ggcgcggtta agccccggat tagcactgat aatcgcctgc
    27601 gcactgctta acgcctggct gctggtttca ctaccggtaa aacgccagcc atattcatgt
    27661 acgccaatca gcggataaat acagttcgca ccaacgccga tatccagaat gctggcattc
    27721 gctggaattg ttccgcttgc ttcagccagt aaatcggcaa ggtgatgaat ataatccgcc
    27781 cggcccggta ctggtgggca gagaaaaccg tcggggatat cccagttcgc tacggcgtaa
    27841 aaatgggcca gcaacgcctt attgagcgcc ttcaccgcca gcggattggc aaagtctacg
    27901 ctttgctccc ctgcgggtgt aagcgtgagg aattgcctga gttcaggatt gacctgacaa
    27961 agcgtggcga gatcgtagcg gctgtgatga cggttgcgcg gatgcaaccc cggtttctgg
    28021 gcggacatag cattctcctt taagcagcgg cgtaagatac ccgcataacg cgacagggta
    28081 aatatctggc tgcgtttatc attccccaaa accttaacat ggctatggtt tatggcaatt
    28141 catcataaaa atcagcatta tgcatgtcat tgatttgtct tattttgtaa aaatatatcg
    28201 ttcaaaaaat aagcacatct ctgtcaattt tttgcgtcat ttgcatcgcg caggcgacct
    28261 acacttatca ggcactaccc gttgtattta tgaggatgtc attatgaaaa agtgcctcac
    28321 actactgatt gccaccgtcc tgagcggaat ctctctcacg gcttatgccg cgcaaccgat
    28381 gagtaacctg gacagcggtc agctacggcc cgccggtacc gtttcggcaa ctggcgcatc
    28441 aaacctaagc gatctggagg ataaactggc agaaaaagcg cgcgaacaag gcgcgaaagg
    28501 ttatgtcatt aattccgcag gcggaaatga ccagatgttc ggtactgcaa ccatctacaa
    28561 ataaccccac caacgtaact gcattcgcct ctggatgcag ttttcgcctg ttttgtaatc
    28621 aaaaaacatc aaatatgaac gcaatgtaaa taaatgtatt tctttttcgc gcaatgggtg
    28681 atagaaaatc gcgtcaagtg ataatgctta tcaaaattat tatcacttgt gcgaacacta
    28741 tcacgggatt aacagtggca tcgcatccgc agagatgctt tctcgtggca gtgaaaattt
    28801 caacatataa gaaaaagtca cctgcaaaat ggaaaacaat cgcaatttcc ctgccagaca
    28861 atttcattcg ctcacgttct ttgccggtct ttgtattggt attacgcctg tggcacaggc
    28921 actcgccgcc gaagggcaag ctaacgcgga tgacacgctg gttgtcgaag catcaacgcc
    28981 ttcgctttat gcgccacaac aatctgccga tccgaaattc tcgcgtccgg tagcggatac
    29041 tacccgcacg atgacggtga tttctgaaca agtgattaaa gatcagggcg caaccaacct
    29101 taccgacgcg ctcaaaaacg tccccggc
//
LOCUS       DADLSA010000038        28483 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.038, whole genome shotgun sequence.
ACCESSION   DADLSA010000038 DADLSA010000000
VERSION     DADLSA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.038"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(9..263)
                     /locus_tag="JN691_001677"
     CDS             complement(9..263)
                     /locus_tag="JN691_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HAY7243143.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPVTLFVPLIMGC"
     gene            573..2345
                     /gene="aspS"
                     /locus_tag="JN691_001678"
     CDS             573..2345
                     /gene="aspS"
                     /locus_tag="JN691_001678"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAY7243144.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2463..2915
                     /gene="nudB"
                     /locus_tag="JN691_001679"
     CDS             2463..2915
                     /gene="nudB"
                     /locus_tag="JN691_001679"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAY7243145.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAQGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2944..3684
                     /locus_tag="JN691_001680"
     CDS             2944..3684
                     /locus_tag="JN691_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7243146.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3719..4240
                     /gene="ruvC"
                     /locus_tag="JN691_001681"
     CDS             3719..4240
                     /gene="ruvC"
                     /locus_tag="JN691_001681"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAY7243147.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQAGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4242..4844)
                     /locus_tag="JN691_001682"
     CDS             complement(4242..4844)
                     /locus_tag="JN691_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HAY7243148.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSLQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4915..4980
                     /locus_tag="JN691_001683"
     CDS             4915..4980
                     /locus_tag="JN691_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243149.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5119..5730
                     /gene="ruvA"
                     /locus_tag="JN691_001684"
     CDS             5119..5730
                     /gene="ruvA"
                     /locus_tag="JN691_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAY7243150.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            5739..6749
                     /gene="ruvB"
                     /locus_tag="JN691_001685"
     CDS             5739..6749
                     /gene="ruvB"
                     /locus_tag="JN691_001685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAY7243151.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6896..7681)
                     /gene="znuB"
                     /locus_tag="JN691_001686"
     CDS             complement(6896..7681)
                     /gene="znuB"
                     /locus_tag="JN691_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAY7243152.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7678..8433)
                     /gene="znuC"
                     /locus_tag="JN691_001687"
     CDS             complement(7678..8433)
                     /gene="znuC"
                     /locus_tag="JN691_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAY7243153.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8512..9444
                     /gene="znuA"
                     /locus_tag="JN691_001688"
     CDS             8512..9444
                     /gene="znuA"
                     /locus_tag="JN691_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAY7243154.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9460..10782
                     /gene="mepM"
                     /locus_tag="JN691_001689"
     CDS             9460..10782
                     /gene="mepM"
                     /locus_tag="JN691_001689"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAY7243155.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10902..11873
                     /gene="lpxM"
                     /locus_tag="JN691_001690"
                     /gene_synonym="msbB"
     CDS             10902..11873
                     /gene="lpxM"
                     /locus_tag="JN691_001690"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7243156.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(12004..13446)
                     /gene="pyk"
                     /locus_tag="JN691_001691"
     CDS             complement(12004..13446)
                     /gene="pyk"
                     /locus_tag="JN691_001691"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAY7243157.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13574..14443)
                     /locus_tag="JN691_001692"
     CDS             complement(13574..14443)
                     /locus_tag="JN691_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY7243158.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14780..16255
                     /gene="zwf"
                     /locus_tag="JN691_001693"
     CDS             14780..16255
                     /gene="zwf"
                     /locus_tag="JN691_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAY7243159.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16490..18301
                     /gene="edd"
                     /locus_tag="JN691_001694"
     CDS             16490..18301
                     /gene="edd"
                     /locus_tag="JN691_001694"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAY7243160.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18338..18979
                     /locus_tag="JN691_001695"
     CDS             18338..18979
                     /locus_tag="JN691_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAY7243161.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(19035..20213)
                     /gene="purT"
                     /locus_tag="JN691_001696"
     CDS             complement(19035..20213)
                     /gene="purT"
                     /locus_tag="JN691_001696"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAY7243162.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20348..20638
                     /gene="yebG"
                     /locus_tag="JN691_001697"
     CDS             20348..20638
                     /gene="yebG"
                     /locus_tag="JN691_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HAY7243163.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            20668..21060
                     /gene="yebF"
                     /locus_tag="JN691_001698"
     CDS             20668..21060
                     /gene="yebF"
                     /locus_tag="JN691_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAY7243164.1"
                     /translation="MLSPESLCYGEKHEKRGAFLGRLLVSACASVFAANNETSKSVTF
                     PKCEGLDAAGIAASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVP
                     LTVRGKSADIHYQVSVDCKAGMAEYQRR"
     gene            21387..22046
                     /locus_tag="JN691_001699"
     CDS             21387..22046
                     /locus_tag="JN691_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAY7243165.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQVIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22255..24315
                     /gene="ptrB"
                     /locus_tag="JN691_001700"
                     /pseudo
     CDS             22255..24315
                     /gene="ptrB"
                     /locus_tag="JN691_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
     gene            complement(24312..24974)
                     /gene="exoX"
                     /locus_tag="JN691_001701"
     CDS             complement(24312..24974)
                     /gene="exoX"
                     /locus_tag="JN691_001701"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAY7243166.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24998..25654)
                     /locus_tag="JN691_001702"
     CDS             complement(24998..25654)
                     /locus_tag="JN691_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAY7243167.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIPGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(25756..25986)
                     /locus_tag="JN691_001703"
     CDS             complement(25756..25986)
                     /locus_tag="JN691_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAY7243168.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26125..26499
                     /gene="yobA"
                     /locus_tag="JN691_001704"
     CDS             26125..26499
                     /gene="yobA"
                     /locus_tag="JN691_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAY7243169.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26503..27375
                     /gene="copD"
                     /locus_tag="JN691_001705"
     CDS             26503..27375
                     /gene="copD"
                     /locus_tag="JN691_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HAY7243170.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLMIRRLLTKRSL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            27388..27729
                     /locus_tag="JN691_001706"
     CDS             27388..27729
                     /locus_tag="JN691_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAY7243171.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28122..>28483)
                     /locus_tag="JN691_001707"
     CDS             complement(28122..>28483)
                     /locus_tag="JN691_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7243172.1"
                     /translation="IAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGIRKNSPDG
                     SIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQRLLGHTSE
                     KTTKMYLDEREKTYLLL"
BASE COUNT         7205 a         7026 c         7396 g         6856 t
ORIGIN      
        1 ctggctggct aacaccccat tataaggggt acgaataaag taacgggggc atcaaccggc
       61 tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa cacgggctga
      121 ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc atcggcagta
      181 tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac caacgcagtg
      241 gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg cctcacacct
      301 taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag ttcgggcaac
      361 acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt ggtaacgagg
      421 cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa tagccgcctt
      481 ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg cagcaggggg
      541 catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt ggacagctcc
      601 gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt cgtcgtgatc
      661 ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag gtatttttcg
      721 atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag ttctgcattc
      781 aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat atggcgaccg
      841 gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat gttctgccgc
      901 ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac ctcgacctgc
      961 gtcgcccgga aatggcgcag cgcctgaaaa cccgcgctaa aatcaccagc ctggtgcgcc
     1021 gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc aaagccacgc
     1081 cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa ttctacgcac
     1141 tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt gaccgttact
     1201 atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag cctgaattta
     1261 ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa gtgatggaag
     1321 cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat ttcccggtaa
     1381 tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg cgtaacccga
     1441 tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta tttgcggggc
     1501 cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc gcatcgctga
     1561 cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg aaaggtctgg
     1621 cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc ccggtagcga
     1681 agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg caagatggcg
     1741 atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg ggcgcactgc
     1801 gcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca ccgctgtggg
     1861 ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca atgcaccatc
     1921 cgttcacctc accgaaagac atgactgctg cagaactgaa agctgcaccg gaaaatgcgg
     1981 tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt tcagtacgta
     2041 tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac gaagaggaac
     2101 agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg ccgcacgcag
     2161 gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac aatatccgtg
     2221 acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa gcaccgagct
     2281 ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag gctgagaata
     2341 actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca actgagtgaa
     2401 ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa cgaagaggca
     2461 gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc tatgcacaag
     2521 atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg cagtcggtaa
     2581 ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa gtaaaggaag
     2641 aggtcaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag cgcacggtag
     2701 agtttgaaat tttttcacat ttacgtcatc gctatgcgca gggcgtgacg cgtaatacgg
     2761 aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact gaacatctgg
     2821 cttacaagtg gcttgatgcg cctgcggcgg cggcgctcac taagtcctgg agcaaccggc
     2881 aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc tggagatatt
     2941 tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc gcaggatgct
     3001 aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc taagctgggc
     3061 ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc actgtctaac
     3121 aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg tgatgatgat
     3181 gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc aatcatgatt
     3241 gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc attcagcaaa
     3301 tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa aaaaggcgtg
     3361 atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga agcaggtgct
     3421 gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg ggaagaaatg
     3481 ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc ggaagtttcc
     3541 atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact gatgcgtctg
     3601 atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg tgaaatctct
     3661 gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga gacgcgtgat
     3721 ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg tcatccgcca
     3781 ggcaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag tggatgattt
     3841 accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc agttccagcc
     3901 tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag ccctgaaact
     3961 gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc cggtatttga
     4021 atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg aaaaaagcca
     4081 ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg cggatgccgc
     4141 cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga tgcagatgag
     4201 cgaatcgcgg ctgaacctgg cgagagggcg actgcgttaa gttataccgc ctcggtcagt
     4261 tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg atgatgccac
     4321 aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa ccagaatttc
     4381 cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt ctctatttca
     4441 cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga acagaactgg
     4501 agcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga gggaacctgt
     4561 tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt tatagcatag
     4621 cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga aaggggaacc
     4681 cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc gacgtgatta
     4741 ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat acatgtaaag
     4801 acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc attccattga
     4861 aatagatacg aatatacgta atagcataaa aaaattattt tttcaggagt cgcaatgtat
     4921 atttttatta cgcatttctt cactgaatat gtaatattca aatatttgct tccaatataa
     4981 cctgttgaat aaattatact gtgccatttt tcagatcatc gggacacctc gcaagttttc
     5041 ttcatccttc gctggatatc tatccagcat ttttttatca tacagcatta tctttgattc
     5101 attacgcagg agcgtcatgt gataggcaga ctcagaggca tcatcattga aaaacaaccc
     5161 ccgctggtgt taattgaagt gggcggcgta ggctatgaag tgcatatgcc gatgacctgt
     5221 ttttatgaac tccctgaagc gggtcaggaa gcgatcgttt tcacccactt tgtggtgcgt
     5281 gaagacgcgc aactgctgta cggttttaac aataaacaag aacgcacgtt gttcaaagag
     5341 ttgatcaaaa ccaacggcgt cggcccgaag ttggccctgg cgatcctctc cggaatgtca
     5401 gcgcagcagt tcgttaatgc cgttgagcgt gaagaagtgg gggcactggt gaaactgccg
     5461 ggtattggca aaaaaaccgc cgaacgcttg attgttgaaa tgaaagaccg atttaaaggt
     5521 ttgcatggcg atctctttac gccagccgcc gacctggtac tcacgtctcc tgccagcccg
     5581 gcgaccgacg atgctgaaca agaagcagtt gccgcgctgg tggcgctagg ctataaacca
     5641 caagaagcaa gccgcatggt gagcaaaatc gctcgccctg acgccagcag tgaaacatta
     5701 attcgcgaag ccctacgcgc cgcgttatga ggtaaaggat gattgaagca gaccgtctga
     5761 tttctgccgg taccactttg ccggaagatg tagcagatcg cgccattcgc cctaaattac
     5821 tggaagagta tgttggtcag ccgcaggttc gttcacagat ggagattttc atcaaagcag
     5881 cgaaactgcg cggcgatgcc ctcgatcatt tgttgatttt tggtcctccg gggttgggta
     5941 aaactacgct tgccaacatt gtcgccaatg aaatgggcgt taatttacgc acgacttctg
     6001 gtccggtgct ggaaaaggcg ggcgatttgg ctgcgatgct cactaacctt gaaccgcatg
     6061 acgtgctgtt tattgatgag atccaccgtt tatcgccagt tgttgaagaa gtgctgtacc
     6121 cggcaatgga agactaccaa ctggatatca tgattggtga aggtccggcg gcacgctcca
     6181 ttaaaattga tttgccgccg tttaccctga ttggcgcaac cacgcgcgca ggttcgctga
     6241 catcaccgtt gcgcgaccgt tttggtattg tgcaacgtct ggagttttat caggtgccgg
     6301 atctgcaata tatcgtcagt cgcagtgcac gctttatggg gcttgagatg agtgatgacg
     6361 gtgcgctgga agtagctcgt cgcgctcgcg gtacgccacg tattgccaac cgtctgctgc
     6421 gtcgagtgcg tgatttcgcc gaagtgaagc acgatggcac catctcggca gatatcgctg
     6481 ctcaggcgct ggatatgttg aatgtcgatg ctgaaggttt cgattatatg gaccgcaaat
     6541 tgttgctggc ggtaatcgat aagttctttg gtgggccggt aggtctggat aacctggcgg
     6601 cagccattgg cgaagaacgt gaaaccattg aagatgtact ggaaccttat ttgattcagc
     6661 aaggcttttt gcagcgtaca ccgcgcgggc gtatggcgac gacgcgggcg tggaatcact
     6721 ttggcataac gccgccagaa atgccgtaag tcggattggt cgctcgcgcc gcatccgaca
     6781 ctggcagtgt gtctgatgcg acactgttcg cgtcttatca ggccagccag agatagcgaa
     6841 ccgtaggtcg gataaggcgc tcgcgccgca tccgacaaat gtgttcagcg atagattagc
     6901 tggcctgctt tttcatcata ctgataataa atagcagtgc cgcacatagc accaccgacg
     6961 gacccgccgg cgtatcgtaa aacgcggaaa aggttaaacc gccagttact gccaccatcc
     7021 ccaccaaaac agcgacacca gccatctgtt ccggcgtacg ggcaaagcga cgcgcagtag
     7081 ctgcaggaat aatcagcaac gaagtaataa tcaacgcacc gacaaatttc atcgctacac
     7141 caatcgtcaa tgccgtcacc agcatcaaca acaatttcac ttgctgtaat ttcacaccat
     7201 caacaaacgc cagatccggg ctaatcgtca tcgacagcaa attgcgccat tgccagaaca
     7261 aaatagccac cacgatgacc acgccaatcg caatagagat gagatcttct ggcgtcactg
     7321 ccagtaaatc accgaacagg taagccatca aatcaacacg aatgttagac atcagactaa
     7381 cgaccactag gcccagcgac agggcactgt gcgccataat ccctaataac gtgtcgatcg
     7441 ccagctgtgg acgcttctcc agccatacca gaccgcccgc cagcagcagc gtaacggcaa
     7501 tcaccgcata gaatggattc acgtccagca acaaaccaaa cgcgacacca agtaatgagg
     7561 catgagccag cgtatcaccg aaataagaca tacgacgcca gactacaaac gaacccagcg
     7621 gacccgcggc acaggcgagc atgatcccgg ctaaccaacc gggaaataat aattcaatca
     7681 tgagcgatca tttccccgac gcaaaacaat tcgtccctgt aaatcgtgac gatgattatg
     7741 atgatggcga tagataccca gttgttcagc accacgagga ccaaacattg aaataaactc
     7801 cggatgcagg gaaacaactt ccggtgtgcc ggaacaacaa atgtggtgat tcaggcacag
     7861 cacttcatcg gtttttgcca tcaccagatg cagatcgtga gaaaccatta aaacgccaca
     7921 atccagttca cgacgcaact ggtcaataag gtcatataac gccacttggc catttacatc
     7981 cacgccttga gtgggttcat ccagcactaa taattgcggg caatttaaca atgctcgcgc
     8041 taatagtaca cgctgcgttt cgccaccaga gagcttttgc atcggtgcgt taatcaggtg
     8101 cccggcctgg acacgtttca gtgcaggcaa aatatcttct ttatgtgtac cagggcgtaa
     8161 gcgtaaaaaa cggtttacgg tcagtggcaa cgtggtgtcg agatacagct tctgcggtac
     8221 atagccgatg cgcagttttc cgttgcgctt gataacccct tcatcgggtg ttaccagccc
     8281 gagcactacc cgtaccagtg tcgacttacc tgcgccattt ggcccaagta aagtcaaaat
     8341 ttttccaggt ttaagttcca gcgacacatc agagaggacg cggcgttggc caaaagaaac
     8401 cgagacattt tccagggaaa ccagacttgt catgttaatt ttagtcttgc agtagtcatg
     8461 aaatgttata atatcacact tctcatattc attacgatga ttggtcgcat tatgttacat
     8521 aaaaaaacgc ttcttttcgc agcattatcc gccgctctct ggggaggtgc aacacaggcc
     8581 gcagatgccg ccgttgtcgc ttcgcttaaa cccgttgggt tcatcgcttc tgccattgct
     8641 gatggtgtaa cagaaacgga ggttttactg cctgacggcg cttcagaaca tgattattca
     8701 ctgcgtccat cggatgtaaa acgcttacag aacgcggact tagtcgtttg ggttggcccg
     8761 gagatggaag cgtttatgca aaaaccggta agcaaattac cagaagcgaa gcaggtaacg
     8821 attgcgcagc ttgaagatgt gaaaccgcta ctgatgaaaa gtattcacgg cgatgatgat
     8881 gatcacgacc acgcggaaaa aagtgacgaa gatcaccatc acggcgattt caacatgcat
     8941 ctttggcttt ccccagagat agcgcgggct acagcggttg caatccatgg aaaattagtg
     9001 gaacttatgc cgcaaagtcg agccaaactt gacgccaacc tgaaggattt tgaggcacaa
     9061 ttggcctcaa ccgaaacgca ggttggtaac gagctcgcgc cgctcaaggg gaaaggttat
     9121 ttcgtttttc acgatgctta cggctatttc gaaaaacagt tcggactgac accgcttggt
     9181 cattttaccg ttaaccctga gattcaacct ggcgcgcagc gtttacatga aataagaaca
     9241 cagttggttg agcaaaaagc aacctgcgtt tttgctgagc cacagttcag gccagcggtc
     9301 gttgagagcg tcgcacgagg gacatccgtt cgtatgggaa cgttggatcc cctggggacg
     9361 aatatcaaac tgggtaaaac aagctattca gaattcctga gtcaattagc caaccagtat
     9421 gcgagctgcc tgaaaggaga ttaatgagga agtgattacg tgcaacagat agcccgctct
     9481 gtcgccctgg cgtttaataa tttaccgcga ccacaccgcg ttatgttggg gtcgctcacc
     9541 gttcttactc tggccgtcgc tgtctggcgg ccctatgttt atcaccgtga tgccacgcca
     9601 attgtcaaaa ccattgagct ggaacagaac gaaattcgtt cgctcttacc tgaagccagt
     9661 gagccgattg atcaagctgc acaagaagat gaagccattc cccaggacga actggatgac
     9721 aaaatcgccg gtgaagcggg cgtgcatgaa tatgttgttt ccactggcga tacgctaagc
     9781 agcattctca atcagtatgg tattgatatg ggtgatatca cccaactggc tgctgccgac
     9841 aaagaattgc gtaacctgaa aatcggtcaa caactctcct ggacattaac cgcggacggc
     9901 gaactgcagc gcctcacctg ggaagtgtct cgtcgtgaaa cccgaaccta tgaccgtact
     9961 gccgctaacg gttttaaaat gaccagcgaa atgcagcaag gagagtgggt taacaatctg
    10021 ctgaaaggta ccgtcggagg gagctttgtt gccagcgcca gaaacgccgg tttaaccagc
    10081 gccgaagtga gcgcagtgat taaagccatg cagtggcaaa tggatttccg caaactgaaa
    10141 aaaggcgatg aatttgcggt attaatgtct cgagaaatgc ttgatggtaa acgtgagcaa
    10201 agccagctgc tgggcgtacg tttgcgttca gaaggtaaag attattacgc aatccgcgct
    10261 gaggatggca aattctacga tcgtaacggt actggtctgg cgaaaggatt cttgcgattc
    10321 ccgacggcga aacagttccg tatctcgtct aactttaacc cgcgtcgaac taatccggtg
    10381 acaggtcgcg ttgcaccaca cagaggtgtt gatttcgcca tgccacaagg tacgccagtg
    10441 ctttcagtgg gtgacggtga agtggtggtt gccaaacgca gtggcgcagc aggttattat
    10501 gtggctattc gtcatggtcg cagctacacc acgcgttata tgcacttgcg caagattctg
    10561 gtgaaaccgg gacagaaggt gaaacgtggc gaccgtatcg cgctttccgg taataccgga
    10621 cgttcaactg ggccgcatct gcactatgaa gtatggataa accagcaggc cgtaaacccg
    10681 ctgacggcaa aactgccgcg taccgaaggg ctgaccggct ccgatcgtcg cgaattcctg
    10741 gcgcaggcca aagagattgt gccgcagcta cggtttgatt aattaacatc cattcgcagc
    10801 cggtacgcag tcagtaccgg ctttttttat ttggtgcggg gcaagttgcg ccgctacact
    10861 atcaccagat tgatttttgc cttatccgaa actggaaaag catggaaacg aaaaaaaata
    10921 atagcgaata cattcctgag tttgataaat cctttcgcca cccgcgctac tggggagcat
    10981 ggctgggtgt agcagcgatg gcgggtatcg ctttaacgcc gccaaagttc cgtgatccca
    11041 ttctggcacg gctgggacgt tttgccggac gactgggaaa aagctcacgc cgtcgtgcgt
    11101 taatcaatct gtcgctctgc tttccagaac gtagtgaagc tgaacgcgaa gcgattgttg
    11161 atgagatgtt tgccaccgcg ccgcaagcga tggcaatgat ggctgagttg gcaatacgcg
    11221 ggccggagaa aattcagccg cgcgttggct ggcaagggct ggagatcatc gaagagatgc
    11281 ggcgtaataa cgagaaagtt atctttctgg tgccgcacgg ttgggccgtc gatattcctg
    11341 ccatgctgat ggcctcgcaa gggcagaaaa tggcagcgat gttccataat cagggcaacc
    11401 cggtttttga ttatgtctgg aacacggtgc gtcgtcgctt tggcggtcgt ctgcatgcga
    11461 gaaatgacgg tattaaacca ttcatccagt cggtacgtca ggggtactgg ggatattatt
    11521 tacccgatca ggatcatggc ccagagcaca gcgaatttgt ggatttcttt gccacctata
    11581 aagcgacgtt gcccgcgatt ggtcgtttga tgaaagtatg ccgtgcgcgc gttgtaccgc
    11641 tgtttccgat ttatgatggc aagacgcatc gtctgacgat tcaggtgcgc ccaccgatgg
    11701 atgatctgtt agaggcggat gatcatacga ttgagcggcg gatgaatgaa gaagtcgaga
    11761 tttttgttgg tccgcgacca gaacaataca cctggatact aaaattgctg aaaactcgca
    11821 aaccgggcga aatccagccg tataagcgca aagatcttta tcccatcaaa taaaaaagcc
    11881 tctcgcgagg agaggccttc gcctgatgat aagttcaagt ttgcttcaga atattcgaaa
    11941 tctgttgaac tatcattgaa ctataggccg gatgtggcgt tttcgccgca tccggcaacg
    12001 tacttactct accgttaaaa tacgcgtggt attagtagaa cccacggtac tcatcacgtc
    12061 gccctgggtg acaatcacca ggtcaccaga catcaagtaa cctttatcgc gcagcagatt
    12121 aaccgcttcg ctggcagctg ctacgccgtc attagcgcta tcaaagtgca ccggcgtaac
    12181 gccacgatag agagcagtca ggttcagcgt acgttcatgg cgcgacatgg cgaaaattgg
    12241 tagaccagag ctgatacggg aggtcatcag cgcggtacga cccgattcgg tcatggtaat
    12301 gatcgccgta acgcccttca ggtggttagc tgcgtacatt gccgacatgg caatagcttc
    12361 ttccacattg tcgaactgaa cgtccagacg gtgtttagaa acgttgatgc tcgggatttt
    12421 ttccgcaccc aggcaaacgc gcgccatggc tgcaacagtt tctgacggat actgcccagc
    12481 ggcagtttct gcagacagca tcacagcgtc agtaccatcc agaacggcgt ttgctacgtc
    12541 catgacttct gcacgcgtcg gcatcgggtt agtaatcatt gactccatca tctgggtcgc
    12601 cgtgattacc gctcggttta gctgacgcgc acgacggatc aacgctttct gaatgccgac
    12661 cagttccggg tcgccaattt ccacaccgag gtcgccacgt gcaaccatta ccacgtcaga
    12721 ggcgaggatg atgtcatcca ttgcatcctg gctgcaaacg gcttccgcac gttcaacctt
    12781 ggcaacaatt ttcgcatcac atcctgcatc gcgtgccagg cgacgggcat agttcagatc
    12841 ttcgccacag cgtgggaagg agacagccag gtaatctacg ccaatcaacg ccgcagtctt
    12901 aatgtctgct ttgtcttttt cggtcagcgc ttcagccgac aaaccgccgc caagtttgtt
    12961 gataccttta ttgttggaga ggggaccacc gacggtgact tcggtgaaca ctttcatgcc
    13021 ctgaacttcc agtactttta actggacgcg accatcgtcc agcagcagga tgtcaccagg
    13081 cacgacgtca gcaggcaggc ctttgtagtc gataccgact ttttctttgt cgccttcacc
    13141 tttacctagg ttggcgtcga gcaggaattt atccccaata ttgaggaaaa ctttgccttc
    13201 tttaaaggtg gatacacgga ttttgggccc ctggaggtca cccagaatag ccacatgacg
    13261 ccccagtttt gcggcaatct cacgaacttt atccgcgcgc attttgtgat cttcaggcga
    13321 gccgtgagaa aagttcatac gtacaacgtt ggcacccgcc gcgataactt tttcaagatt
    13381 attatcgcga tctgttgctg ggcctaacgt ggtaacgatt tttgttctgc gaagcctttt
    13441 ggacatgtaa tactccgttg actgaaacaa ccaggtgttg cttgaacatg aaatccgaat
    13501 gaaataacgc cgcgatggtg ataaattaat ctcattcgga tgtcgtataa gattaggaca
    13561 gtgacagtcg cttttagcga tcgtcactta aattaagtaa ctgcttatca aaacgcgatt
    13621 ccttcagcgc ttccttgacc cgcttcaagt tatctctgaa ttttgcaccg cgtcgcaaag
    13681 taaatcctgt cgccagcaca tctatcacgg tcagctgtgc aagtcgagaa accatgggca
    13741 tataaatgtc agtatcttcc ggtacgtcga gggtaattgc cagcgttgct tcccgggcga
    13801 gcggggtacc cgcagaggtg agggcaatca ccatggcgtc gttttcgcgt gccagctgcg
    13861 ccagctcgac cagatttttt gttcttcctg tgtgagaaat cagcaccacc acgtctccgt
    13921 cgctacaatt catacaactc atgcgttgca gcacgatatc atcggagtac accaccggaa
    13981 cattaaaacg aaagaactta ttcatcgcat cgtgggcaac ggcggcagaa gagcctaatc
    14041 cgaaaaaggc gatttttttt gcctgagtga gcaagtcgac ggcgcggttg atggcagatt
    14101 tatccagtga atgacggaca tgatcgagcg ttgccattgc ggactcaaat attttccccg
    14161 tgtatgattc aacgctgtca tcttcattga cgttgcgatt aacataggga gtgccattcg
    14221 ccagactttg tgccagatga agtttaaaat caggaaaacc gcgcgtgtcc atgctgcgac
    14281 agaaacgatt caccgtcggt tcgctaacat tggcttccag cgccagagca gcaatactcg
    14341 aatggatcgc gttatcgggc gaagccagaa tgacctcggc aactttgcgc tctgatttgc
    14401 tcaaatgttc cagctgagac tggatttttt ccagcatatt catgatgtaa agagactcac
    14461 gggtaatgac gatttccgca ctgaaagaaa tcgaaatgca gttttgtcag atattacgcc
    14521 tgtgtgccgt gttaatgaca aaagcagata aaaaagttgt tatttttttc ataacatgat
    14581 cagtgtcaga tttttaccga atggaaaacg atgatttttt tatcagtttt gccgcacttt
    14641 gcgcgctttt cccgtaatcg cacgggtgga taagcgttta cagttttcgc aagctcgtaa
    14701 aagcagtaca gtgcaccgta agaaaattac aagtataccc tggcttaagt accgggttag
    14761 ttaacttaag gagaatgaca tggcggtaac gcaaacagcc caggcctgtg acctggtcat
    14821 tttcggcgcg aaaggcgacc ttgcgcgtcg taaattgctg ccttccctgt atcaactgga
    14881 aaaagccggt cagctcaacc cggacacccg gattatcggc gtagggcgtg ctgactggga
    14941 taaagcggca tataccaaag ttgtccgcga ggcgctcgaa actttcatga aagaaaccat
    15001 tgatgaaggt ttatgggaca ccctgagtgc acgtctggat ttttgtaatc tcgatgtcaa
    15061 tgacactgct gcattcaacc gtctcggcgc gatgctggat caaaaaaatc gtatcaccat
    15121 taactacttt gccatgccgc ccagcacttt tggcgcaatt tgcaaagggc ttggcgaggc
    15181 aaaactgaat gctaaaccgg cacgcgtagt catggagaaa ccgctgggga cgtcgctggc
    15241 gacctcgcag gaaatcaatg atcaggttgg cgaatacttc gaggagtgcc aggtttaccg
    15301 tatcgaccac tatcttggta aagaaacggt gctgaacctg ttggcgctgc gttttgctaa
    15361 ctccctgttt gtgaataact gggacaatcg caccattgat catgttgaga ttaccgtggc
    15421 agaagaagtg gggatcgaag ggcgctgggg ctattttgat aaagccggtc agatgcgtga
    15481 catgattcag aaccacctgc tacaaattct ctgcatgatt gcgatgtctc cgccatctga
    15541 cctgagcgca gacagcatcc gcgatgaaaa agtgaaagta ctgaagtccc tgcgccgcat
    15601 cgaccgctcc aacgtacgcg aaaaaaccgt acgcgggcaa tatactgcgg gctttgccca
    15661 gggcaaaaaa gtgccgggat atctggaaga agagggcgcg aacaaaagca gcaatacaga
    15721 aaccttcgtg gcgatccgcg tcgacattga taactggcgc tgggccggtg tgccattcta
    15781 cctgcgtact ggtaaacgtc tgccgaccaa atgttctgaa gtcgtggtct atttcaaaac
    15841 acctgaactg aatctgttta aagagtcgtg gcaggatctg ccgcagaata aactgactat
    15901 ccgtctacaa cctgatgaag gcgtggatat ccaggtactg aataaagttc ctggccttga
    15961 ccacaaacat aacctgcaaa tcaccaagct ggatctgagc tattcagaaa cctttaatca
    16021 gacgcatctg gcggatgcct atgaacgttt gctgctggaa accatgcgtg gtattcaggc
    16081 actgtttgta cgtcgcgacg aagtggaaga agcctggaaa tgggtagact ccattactga
    16141 ggcgtgggcg atggacaatg atgcgccgaa accgtatcag gccggaacct ggggacccgt
    16201 tgcctcggtg gcgatgatta cccgtgatgg tcgttcctgg aatgagtttg agtaatatct
    16261 gtgcttatcc tttatggtta ttttaccggt aacatgatct tgcacagatt gtagaacaat
    16321 ttttacactt tcaggcctcg tgcggattca cccacgaggc tttttttatt acactgactg
    16381 aaacgttttt gccctatgag ctccggttac aggcgtttca ctcataaatc ctctgaatga
    16441 aacgcgttgt gaatcatcct gctctgacaa ctcaatttca ggagccttta tgaatccaca
    16501 attgttacgc gtaacaaatc gaatcattga acgttcgcgc gagactcgct ctgcttatct
    16561 cgcccggata gaacaagcga aaacgtcgac cgttcatcgt tcgcagttgg catgcggtaa
    16621 cctggcacac ggtttcgctg cctgccagcc agaagacaaa gcctctttga aaagcatgtt
    16681 gcgtaacaat atcgccatca tcacctccta taacgacatg ctctccgcgc accagcctta
    16741 tgaacactat ccagaaatca ttcgtaaagc cctgcatgaa gcgaatgcgg ttggtcaggt
    16801 tgcgggcggt gttccggcga tgtgtgatgg tgtcacccag gggcaggatg gaatggaatt
    16861 gtcgctgcta agccgcgaag tgatagcgat gtctgcggcg gtggggctgt cccataacat
    16921 gtttgatggt gctctgttcc tcggtgtgtg cgacaagatt gtcccgggtc tgacgatggc
    16981 agccctgtcg tttggtcatt tgcctgcggt gtttgtgccg tctggaccga tggcaagcgg
    17041 tttgccaaat aaagaaaaag tgcgtattcg ccagctttat gccgaaggta aagtggaccg
    17101 catggcctta ctggagtcag aagccgcgtc ttaccatgcg ccgggaacat gtactttcta
    17161 cggtactgcc aacactaacc agatggtggt ggaatttatg gggatgcagt tgccaggctc
    17221 ttcttttgtt catccggatt ctccgctgcg cgatgctttg accgctgccg ctgcgcgtca
    17281 ggttacacgc atgaccggta atggtaatga atggatgccg atcggtaaga tgatcgatga
    17341 gaaagtggtg gtgaacggta tcgttgcact gctggcgacc ggtggttcca ctaaccacac
    17401 catgcacctg gtggcgatgg cgcgcgcggc cggtattcag attaactggg atgacttctc
    17461 tgacctttct gatgttgtac cgctgatggc acgtctctac ccgaacggtc cggccgatat
    17521 taaccacttc caggcggcag gtggcgtacc ggttctggtg cgtgaactgc tcaaagctgg
    17581 cctgctgcat gaagatgtca atacggtggc aggctttggt ctgtctcgtt atacccttga
    17641 accatggctg aataatggtg aactggactg gcgggaaggg gcggaaaaat cactcgacag
    17701 caatgtgatc gcttccttcg aacaaccttt ctctcatcat ggtgggacaa aagtgttaag
    17761 cggtaacctg ggccgtgcgg ttatgaaaac ctctgccgtg ccggttgaga accaggtgat
    17821 tgaagcgcca gcggttgttt ttgaaagcca gcatgacgtt atgccggcct ttgaagcggg
    17881 tttgctggac cgcgattgtg tcgttgttgt ccgtcatcag gggccaaaag cgaacggaat
    17941 gccagaatta cataaactca tgccgccact tggtgtatta ttggaccggt gtttcaaaat
    18001 tgcgttagtt accgatggac gactctccgg cgcttcaggt aaagtgccgt cagctatcca
    18061 cgtaacacca gaagcctacg atggcgggct gctggcaaaa gtgcgcgacg gggacatcat
    18121 tcgtgtgaat ggacagacag gcgaactgac gctgctggta gacgaagcgg aactggctgc
    18181 tcgcgaaccg cacattcctg acctgagcgc gtcacgcgtg ggaacaggac gtgaattatt
    18241 cagcgccttg cgtgaaaaac tgtccggtgc cgaacagggc gcaacctgta tcacttttta
    18301 agacgacaaa tttgtaatca ggcgagagaa aactctgatg aaaaactgga aaacaagtgc
    18361 agaatcaatc ctgaccaccg gcccggttgt accggttatc gtggtaaaaa aactggaaca
    18421 cgcggtgccg atggcaaaag cgttggttgc tggtggggtg cgcgttctgg aagtgactct
    18481 gcgtaccgag tgtgcagttg acgctatccg tgctatcgcc aaagaagtgc ctgaagcgat
    18541 tgtgggtgcc ggtacggtgc tgaatccaca gcagctggca gaagtcactg aagcgggtgc
    18601 acagttcgca attagcccgg gtctgaccga gccgctgctg aaagctgcta ccgaagggac
    18661 tattcctctg attccgggga tcagcactgt ttccgaactg atgctgggta tggactacgg
    18721 tttgaaagag ttcaaattct tcccggctga agctaacggc ggcgtgaaag ccctgcaggc
    18781 gatcgcgggt ccgttctccc aggtccgttt ctgcccgacg ggtggtattt ctccggctaa
    18841 ctaccgtgac tacctggcac tgaaaagcgt gctgtgcatc ggtggttcct ggctggttcc
    18901 ggcagatgcg ctggaagcgg gcgattacga ccgcattact aagctggcgc gtgaagctgt
    18961 agaaggcgct aagctgtaaa agtcaaaatg cccgatctag gatcgggcat ttttgtagcg
    19021 tttttttgcc gggtttaacc ctgtactttt acctgtccgg cggcgtgctt cgcgcgttca
    19081 atggcgtcaa caacactctc tgaagtagcc agtaccaccc ccagacgacg gctgccatca
    19141 atttccggct taccaaataa acgaatctgc aaatctgcgc ctacggcatt ctgcacatta
    19201 tcaaacgtga cattctgact ggtcagttgt ggcagaataa cggcagaagc tgcaggacca
    19261 tactgacgga tcccgccaac aggaagtccg aggaaggcac gtacatgcag ggcaaactct
    19321 gagagatctt gagaaattaa cgtcaccatc ccggtatcat gtggacgagg ggagacctca
    19381 ctgaaaatca cctcatcacc acagacaaac agctcgacac caaacagacc ataaccgccc
    19441 agtgccagca ccactttacg ggcaatctcc tgcgcacgtt caagggcaag cgggctcatt
    19501 tgctgtggtt gccaggattc acggtagtcg ccatcttcct ggcgatgacc tactggtgca
    19561 cagaaatgga cgccatccac cgcgctgacg gttagcaggg taatttcgaa gtcaaactta
    19621 acgacgcctt caacaattac gcggcccgct ccggcgcgac cgccttgctg agcgtattcc
    19681 catgcctgag caagttgctc tgcagagcga ataaacgtct gccccttgcc ggaagagctc
    19741 atcaccggtt ttacaatgca gggatagcca atggcggcaa ccgcctcgcg gaaaaggctt
    19801 tcgctatcgg caaaacgata agtggaagtg ggcagctgca gctcttctgc cgccaggcga
    19861 cggataccct cgcgattcat cgttaatttc gttgcgcgag cgcaggggac aacattcagt
    19921 ccttcctctt caagttggat cagcatatcg gtggcaatag cttcgatctc cggcacgata
    19981 taatgtggtt tttccagttc aaccacacgg cgtaatgcat caccatcaag catattaatg
    20041 acatgggagc gatgcgcgac atgcatggct ggtgcgtcgg catagcgatc gacggcaatc
    20101 acctctacgc cgagacgctg acactcgatt gccacttctt tacccagttc accggagcct
    20161 aataacatca cgcgagttgc tgccggacgc agcgcagtgc ctaataacgt cataacgaat
    20221 atcccctgat taattccgcg cgcagtataa acgaaaacgt ttgcgtgtgt ctttattgct
    20281 gatgctgatt tctcaaccga aaagaaatat actgatataa aatcacagtt attatgagag
    20341 gctgcaaatg gcggttgaag ttaaatacgt agtcattcgt gagggcgaag agaaaatgtc
    20401 gtttaccagc aaaaaggaag ccgatgctta tgacaaaatg cttgatacgg cggatctgct
    20461 tgacacctgg ctgacaaatt ctccagtgca aatggaagac gagcaacgtg aagccctttc
    20521 gctatggctg gcagaacaaa aagatgtgct gagcaccatt ctgaaaaccg gcaaattacc
    20581 gtccccacag gtcgtcggtg ctgaatccga agaggaagat gcctcacacg cggcctgaag
    20641 caacttgcgc tcctgacaat ttgtaccatg ctttctcctg aatcattgtg ctatggagaa
    20701 aaacatgaaa aaagaggggc gtttttaggg cggttgttgg tttctgcctg cgcatcagtt
    20761 ttcgctgcca ataatgaaac cagcaagtcg gtcactttcc caaagtgtga aggtctggat
    20821 gctgccggaa ttgccgcgag cgtaaaacgt gattatcaac aaaatcgcgt ggcgcgctgg
    20881 gcagatgatc aaaaaattgt cggtcaggcc gatcccgtgg cctgggtcag tttgcaggac
    20941 attcagggta aagatgataa atggtcagta ccgctaaccg tgcgtggtaa aagtgccgat
    21001 attcattacc aggtcagcgt ggactgcaaa gcgggaatgg cggaatatca gcggcgttaa
    21061 tggcttctga taattcgatt gaccgttggc ggatgcggcg cgagtgcctt gtccgcccca
    21121 gaacgcacca caatcgtagg cctgagaaga cgcaacagcg tcgcatcggg caatcagcat
    21181 caaacaatgc cactcaaaac gacaaactac tttcacctaa aataccctta tcacctgata
    21241 tacagtaaat accttacctg gcgcgaattt ctttgaataa cggcctaccg ccccgcttat
    21301 ttgcgcatct tttgtctcga tatacatttt ctgacatcct catccgctat gctacaggca
    21361 ctaaaaatgg ttattgagga gcaaatatgg ctaactggtt aaatcaactg caatccctgc
    21421 ttgggcaaag cagttcttct acctcctcgt ctgcggatca gggattgggc aaactgttag
    21481 tgccaggcgc attaggcgga ctggctgggc tgctggtcgc aaataaatca gcacgtaaac
    21541 ttcttacgaa atatggcacc aacgcgttac tggttggcgg cggagcggta gcgggtacgg
    21601 tgctgtggaa taaatacaaa gataaaattc gcgcggcgca tcaggacgaa ccgcagtttg
    21661 gcgcgcaaag tacgccgctg gatgagcgta cggaacgttt gatccttgcg ctggtctttg
    21721 ccgctaaaag tgatggtcat attgatgcca atgaacgtgc ggcaatcgac cagcaattgc
    21781 gtgaagccgg cgtggaagag caggggcgtg tactcattga gcaggtaatc gaacaaccgc
    21841 tggatccaca acgcctggct accggggtcc gcaatgaaga agaggcgctg gaaatctatt
    21901 tcctgagctg cgcggctatt gatattgacc attttatgga acgcagttac ctgaatgcac
    21961 tgggtgacgc gctgaaaatc ccccaggatg ttcgtgaagg cattgaacgc gatctcgaac
    22021 agcaaaaacg cactttggcg gaataattcc gctctctgga tagcctgaag ttgactattt
    22081 cgggctatcc agaaccgcac agccatttga acaggctgat tttctctgtc tgcataacat
    22141 tcgatatccg cgccattacc tgtcatcatc taagcaatga cgaccctgtt tcgcttgcat
    22201 ccccggtgag ttttgccacc cttataagat gtttcaacca gaaagaacaa taacatgcta
    22261 ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg catcgataat
    22321 tactactggc tgcgggacga tacgcgttct cagccagaag tcctggacta cctgcaacaa
    22381 gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga tcgcatctta
    22441 aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat aaaaaatggc
    22501 taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca gcgtcaatcg
    22561 gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa gcgcgcagct
    22621 catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac cattatggcg
    22681 ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa tctggaaaca
    22741 ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg ggcaaatgac
    22801 tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta tcaggtctgg
    22861 cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga aaaagacgat
    22921 acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat tcatctggcc
    22981 agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc cgagccgttt
    23041 gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca gcatcgtttt
    23101 tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg tatgcgtgat
    23161 gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga agggtttacg
    23221 ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag tttgcgccaa
    23281 attaaccgca agacccggga agtcattggc attgcctttg atgatccggc ctatgtgacc
    23341 tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta ttcttccatg
    23401 accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg tgtattaaaa
    23461 caaacagaag tgcccggttt tgatgcggcg aattaccgca gtgaacacct gtggatagtc
    23521 gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca ttttcgcaaa
    23581 ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat tgatgccgat
    23641 ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat tgtccatgtt
    23701 cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct gaagaagaaa
    23761 aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg ctatggctct
    23821 ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg cgttgcaatt
    23881 aattaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt tgatgttgta
    23941 acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga gtgggggaat
    24001 ccgcagggtc cgcaatatta cgagtacatg aaaagctaca gcccgtatga caacgtcacc
    24061 gcacaggctt atccgcattt actggtaacg accggtttac acgattctca ggtgcaatat
    24121 tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga ccatctttta
    24181 ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt taaatcgtac
    24241 gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac attacccgct
    24301 acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag ctccgggctc
    24361 atgctgtcca ggttattaaa taaccagcgc agatagcccg gatcgcgttc ggcaacgtcg
    24421 gaaaccgctt tgccacggta tttgccaaag gtgaaggtcg tcatcaacga cggacgtccg
    24481 gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc gataagcaac
    24541 gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccgg cggtgtctgt
    24601 acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc tggccacaaa
    24661 cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag tacgcggcgg
    24721 tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg gatcacatct
    24781 tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc catcgcttgt
    24841 ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat ttttccgtca
    24901 atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt ttctgtatcg
    24961 ataatgcgca acatggtatg ctccaggccg aaaaagccta gcgtaatgga atgatatcgc
    25021 cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga tcggcgcgaa
    25081 cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt gccatcagca
    25141 cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc agcaggtcgg
    25201 gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata tccatacctt
    25261 gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat gcaccatgac
    25321 tctgatggta gatcccaggc gtttttcgcc acggtgcgaa caccgcaatt ccaggaataa
    25381 agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgccag gtcgttgcgg
    25441 cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca cgtcgcgaat
    25501 aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt tgcctggcgg
    25561 cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt ttacgaggct
    25621 catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca aaatgactgc
    25681 aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt aagaaagtgt
    25741 aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca gattgaccga
    25801 agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt ctggctgttc
    25861 acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag ccaccccggc
    25921 cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag ccagattctt
    25981 cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga gcctggatgg
    26041 caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc tgtaaaaaag
    26101 ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg ctacgcgctg
    26161 gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca tttaacgcat
    26221 cagtatcctg cggcaaacgc gcaagtgaca gctgccccgc aggcaatcac tttaaacttc
    26281 tcggaaggcg ttgaaacagg attcagtggt gcaaaaatca cggggccaaa aaacgaaaat
    26341 attaaaacat taccagcgaa gcggaatgag caggaccaaa agcaactgat tgttccactg
    26401 gccgattcgc tgaaacccgg aacctacacc gttgactggc atgttgtttc ggtggacggg
    26461 cataaaacga aaggacacta cacctttagc gtgaaataaa tcatgctggc gtttacctgg
    26521 atcgcgctgc gatttatcca tttcacctcg ttgatgctgg tctttggctt tgcaatgtat
    26581 ggtgcctggc tggccccctt aatgattcgt cgcttgctga cgaagcgttc tctgcgttta
    26641 caacagcacg ctgccgtctg gagtttgatc agtgccactg caatgctcgc cgttcagggc
    26701 gggttgatgg gaacgggatg gacagatgta ttttcgccaa acatttggca ggctgttttg
    26761 caaacgcagt ttggtggcgt ctggttatgg caaattgttc tcgccctcgt aacgttgatc
    26821 gtcgccctta tgcaaccgcg gaatatgcca cgtttgctgt ttatgctcac taccgcgcaa
    26881 tttattctgc tggcgggagt ggggcatgcg acgctgaatg aaggggtaac agcgaaaatc
    26941 catcagacta atcacgcgat tcacctgatt tgtgcggcgg cgtggtttgg gggattactg
    27001 ccggtgctct ggtgtatgca actcatcaaa ggccgttggc gacaccaggc tattcaggcg
    27061 ctgatgcgtt tttcctggtg cgggcatttt gcggtgatcg gcgtactggc aagcggcgtg
    27121 cttaatgcat tgttgattac cggatttccc cccacgctta cgacttactg gggccaactc
    27181 ttattgctca aagccatctt ggtgatgatt atggtggtca ttgcgctggc taatcgttat
    27241 gttcttgtac cgcgcatgag gcaggatgaa gatcgtgcgg caccctggtt tgtgtggatg
    27301 actaaactcg aatgggcgat aggtgcggta gtgctggtga tcatcagcct gcttgcgact
    27361 ctcgaacctt tttgatggat tacgaaaatg atgaaaaaaa gtattctggc gtttctgtta
    27421 ctcaccagtt ctgcagcggc gctggcggct ccacaggtga taaccgtgag tcgttttgaa
    27481 gtgggtaaag acaaatgggc gtttaatcgc gaagaggtaa tgctgacttg ccgaccgggt
    27541 aatgctttgt atgtcatcaa cccaagtacc ctcgtgcagt atcctttaaa cgatatcgca
    27601 caaaaggaag ttgccagtgg gaagaccaat gcccaaccca tttcggtgat tcagattgat
    27661 gatcctaaca atcccggcga aaaaatgagt ctggcaccgt ttatagaacg agctgaaaaa
    27721 ctctgttaat tacctaaaat agccttttga tttccaataa aaaaaccgcc tcagttcttt
    27781 caccagaacg ggcggttttt aacatttaag ctgatgacca ccgcgctttt tattgaccat
    27841 tttgcacgca aactggaaaa cctggcgtcg tcatctattc ttaaagggca aggcaactaa
    27901 gcctgcatta atgccaactt ttagcgcacg gctctctccc aagagccatt tccctggacc
    27961 gaatacagga atcgtattcg gtcttttttt aattgcattt gaaatcaatt agttgcaaat
    28021 gtctcccacg aaattccccg aaatttactc gaatttctgt attccggtct ttttggttat
    28081 atcacaacca aaatacattt aacaatccat ttacgttaaa atcagagcag taagtacgtt
    28141 ttttctctct catcaagata catttttgtt gtcttctccg atgtgtggcc aagtagacgc
    28201 tgagcaaatt cttctccaca tgtttctttg tacaatcgtc cagccaggct tctgatctcg
    28261 tgaaaagttg gtgggttttc actgaactgg atacctgtga attttctggc tgcgacaaat
    28321 ttttttgtca ggccatccgg gtgaatgctg ccgtcagggc tgttttttct aatcccggca
    28381 ctgattagat aatctccccg gcttaccagg cggcatcgtt caactactgt accaagccgt
    28441 agaccagcga caggaaggct gagtgacagg gggatagcaa tca
//
LOCUS       DADLSA010000039        27699 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.039, whole genome shotgun sequence.
ACCESSION   DADLSA010000039 DADLSA010000000
VERSION     DADLSA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.039"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..613
                     /locus_tag="JN691_001708"
     CDS             <1..613
                     /locus_tag="JN691_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7243173.1"
                     /translation="MDIHSYHCEGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQ
                     ELTEVAELITSPEAIKNNLAVAFLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHAS
                     LQQRWSVADMAATIPCSEAWLRRLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGE
                     VADTLNFFDSFHFSKAFKHKFGYAPSAVLKNTDQHPTDASPHN"
     gene            complement(610..1424)
                     /locus_tag="JN691_001709"
                     /pseudo
     CDS             complement(610..1424)
                     /locus_tag="JN691_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inactive 6-phospho-alpha-glucosidase"
     gene            complement(1424..3040)
                     /locus_tag="JN691_001710"
     CDS             complement(1424..3040)
                     /locus_tag="JN691_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY7243174.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3335..4009
                     /locus_tag="JN691_001711"
     CDS             3335..4009
                     /locus_tag="JN691_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7243175.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(4048..5709)
                     /locus_tag="JN691_001712"
     CDS             complement(4048..5709)
                     /locus_tag="JN691_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAY7243176.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQQVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(5905..6333)
                     /gene="ibpB"
                     /locus_tag="JN691_001713"
     CDS             complement(5905..6333)
                     /gene="ibpB"
                     /locus_tag="JN691_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAY7243177.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6445..6858)
                     /gene="ibpA"
                     /locus_tag="JN691_001714"
     CDS             complement(6445..6858)
                     /gene="ibpA"
                     /locus_tag="JN691_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAY7243178.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            7163..7495
                     /locus_tag="JN691_001715"
     CDS             7163..7495
                     /locus_tag="JN691_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY7243179.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7497..8747)
                     /locus_tag="JN691_001716"
     CDS             complement(7497..8747)
                     /locus_tag="JN691_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HAY7243180.1"
                     /translation="MMAGTVLYQDRAMKQITFAPRNHLLTNTNTWTPDSQWLVFDVRP
                     SGASFTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWH
                     YDFHHRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVM
                     HQLDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAY
                     EEGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPP
                     RGVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGE
                     PRKLTHNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAV
                     VFSPDGQWLAWMEGGQLWITETDR"
     gene            8812..9912
                     /gene="cbrA"
                     /locus_tag="JN691_001717"
     CDS             8812..9912
                     /gene="cbrA"
                     /locus_tag="JN691_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HAY7243181.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSDAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(9873..11210)
                     /locus_tag="JN691_001718"
     CDS             complement(9873..11210)
                     /locus_tag="JN691_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7243182.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11285..12433)
                     /gene="dgoD"
                     /locus_tag="JN691_001719"
     CDS             complement(11285..12433)
                     /gene="dgoD"
                     /locus_tag="JN691_001719"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAY7243183.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPFGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(12430..13047)
                     /locus_tag="JN691_001720"
     CDS             complement(12430..13047)
                     /locus_tag="JN691_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAY7243184.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13031..13909)
                     /locus_tag="JN691_001721"
     CDS             complement(13031..13909)
                     /locus_tag="JN691_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HAY7243185.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(13906..14595)
                     /gene="dgoR"
                     /locus_tag="JN691_001722"
     CDS             complement(13906..14595)
                     /gene="dgoR"
                     /locus_tag="JN691_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HAY7243186.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            14873..15529
                     /locus_tag="JN691_001723"
     CDS             14873..15529
                     /locus_tag="JN691_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243187.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(15575..16388)
                     /gene="yidA"
                     /locus_tag="JN691_001724"
                     /pseudo
     CDS             complement(15575..16388)
                     /gene="yidA"
                     /locus_tag="JN691_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
     gene            complement(16502..16909)
                     /locus_tag="JN691_001725"
     CDS             complement(16502..16909)
                     /locus_tag="JN691_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAY7243188.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(17130..19544)
                     /gene="gyrB"
                     /locus_tag="JN691_001726"
     CDS             complement(17130..19544)
                     /gene="gyrB"
                     /locus_tag="JN691_001726"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAY7243189.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19573..20646)
                     /gene="recF"
                     /locus_tag="JN691_001727"
     CDS             complement(19573..20646)
                     /gene="recF"
                     /locus_tag="JN691_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAY7243190.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPEFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20646..21746)
                     /gene="dnaN"
                     /locus_tag="JN691_001728"
     CDS             complement(20646..21746)
                     /gene="dnaN"
                     /locus_tag="JN691_001728"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAY7243191.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(21751..23154)
                     /gene="dnaA"
                     /locus_tag="JN691_001729"
     CDS             complement(21751..23154)
                     /gene="dnaA"
                     /locus_tag="JN691_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAY7243192.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23451..23531
                     /locus_tag="JN691_001730"
                     /pseudo
     CDS             23451..23531
                     /locus_tag="JN691_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23761..23901
                     /gene="rpmH"
                     /locus_tag="JN691_001731"
     CDS             23761..23901
                     /gene="rpmH"
                     /locus_tag="JN691_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAY7243193.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            23918..24277
                     /gene="rnpA"
                     /locus_tag="JN691_001732"
     CDS             23918..24277
                     /gene="rnpA"
                     /locus_tag="JN691_001732"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAY7243194.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24241..24498
                     /gene="yidD"
                     /locus_tag="JN691_001733"
     CDS             24241..24498
                     /gene="yidD"
                     /locus_tag="JN691_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAY7243195.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24501..26147
                     /gene="yidC"
                     /locus_tag="JN691_001734"
     CDS             24501..26147
                     /gene="yidC"
                     /locus_tag="JN691_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAY7243196.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26253..27617
                     /gene="mnmE"
                     /locus_tag="JN691_001735"
     CDS             26253..27617
                     /gene="mnmE"
                     /locus_tag="JN691_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAY7243197.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEACQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6699 a         7465 c         7050 g         6485 t
ORIGIN      
        1 aatggatatt cactcttatc actgtgaagg tttagtctgg gaacagtact ggatggaatt
       61 tacccccacc agtatgatgg atattcccgt tggtcagcaa agcgttattt ataatggcga
      121 aatttataat caggaactca ccgaagttgc tgagttaata acttcaccag aagcaataaa
      181 aaataatctg gcagtcgctt ttctgacgaa aattatttat cagtggattt gtcttatgta
      241 cgcagatggt aaaaaagatc cacaacggcg gcaaattgaa aaattaattg ccactttaca
      301 tgccagtctg caacaacgct ggagcgtagc tgatatggct gccacgatcc cctgtagcga
      361 agcctggttg cgtcgtctgt ttttacgcta taccggcaag acgccgaaag aatattacct
      421 cgatgcgcgt ctggatctgg cgctatcgct attaaagcaa caaggaaact cggttggcga
      481 agtcgctgat acgctcaact tcttcgactc ctttcatttc agcaaagcct ttaaacataa
      541 atttggttat gcgccgtcag ccgtgctaaa gaatacggac cagcacccaa cggatgccag
      601 tccacacaat taacgtaact ctggccagta cgctttgttg gcctccagca actcatccag
      661 aatagctttg gcgaccgagg cgctcggtac cgttttcgac aacgtaatcg cctgccacag
      721 gtgctgatat gaacgctgtt cccaggcgtc cacgaccagt ttttccaccg ccacttgctg
      781 actcattaac cctttctgaa actgcgggat atcgccgacc accagtggtt caggccgtag
      841 tagcgcacgc gcatctgttt gcgatcttgc agcccaacaa tttgcgccat ccggctttca
      901 ataccgattg gcatgtcaca gatgttgagg attttcgcat tcgggcgcag acgacgcgag
      961 gcttctgcga caattgctgc cgggttggag tagttgagca tccaggcatt tggtgaatat
     1021 ttttccatat aatccaccag ttccaggacg ccgccaatgg aacgcatgcc gtacgctatt
     1081 ccgccgggtc cgcaagtttc ctgaccaaca acaccgtggc gcagtgggat tttttcatcc
     1141 agttcgcgca tcgggtactt gccgacgcgg atgtgcgcca tgacaaaatc aacgtcgctg
     1201 aacgccactt caggatcggt ggtgtaacta aacgcaatgt ccggcgcttt ttctttaagg
     1261 atgactttac aggcttcggc aatcacttcc tgccgcgcgc catcgttatc ataaaatttc
     1321 agtgcacgaa gcgggaaacg gtcctgattc gccaggagca tcaacacgat gcctggcgta
     1381 aaggtgcttc caccgcctgc gacaaccact gagaatttgg tcattatact gcctccgtaa
     1441 tggcaacatt ttctgctgat tgatgagaat taattaagct atcgagctgt tcacgcaatt
     1501 gggatacatg cagaccaatg atcacctgaa tggcatcgcc actacggaag acgccgtgcg
     1561 ctcccagctt tttaaagact tcgtcatcca gcgtttgtga catgtcatgc agtgcaatac
     1621 gtagacgcgt cgcgcaattg ttaatgctgg agatattgcc gaccccgccc agggcttgca
     1681 ggataccggc agcctgatcc agctcttttt ttggctctgc agcggtggtt tggcctcgcg
     1741 aggctttgta ttcggctttt gagtagagtt tcacttccgc atcttcacgt cccggcgtgc
     1801 acatgttgaa ctgcagaatc agggtgcgga aaaccacgaa gtacagcagg gtaaagcaca
     1861 acccaatggc gatttgggtc agcatcatat ccgcgtggtt gctgaacatc gggatccagt
     1921 tttgcggtaa aacctggtca atcagacctc cgcccatgtt gcccaccaca ccaaagagat
     1981 acattacggt cgacattgag gccgccagca cggcgtgtac cgcaaacagc aacggtgaaa
     2041 tgaacaggaa ggtaaattcc agcggttcgg taattcccac cagcatggcg gttaaggttg
     2101 cgggaatcag caagcccgcc acttttaccc gattttccgg tgcggcagtg aagtacatcg
     2161 ctaaagaaat gcccacggca ccaaagattt ttgagttacc gtgcagggca aaaccgcctt
     2221 ccgggaacaa cgatttcagc ggctcggcgc tcagactgaa ctcttgcaga tgctgcgccc
     2281 agtacatctg aatgccgcct tcaacagctg ccggaccaaa gataaacggt ccgtagatga
     2341 agtggtgtaa accggttggg atcagaatac gttcgaggaa ggtgtaaacc cacaccccaa
     2401 gcgcacccgc cgaacgcagg aacgcttgca gagattcaat ccccatttgt acttttggcc
     2461 agccgagcaa tgtcagccag gcacagggga tcatcaccag gaaggcgata atcaccacat
     2521 aagacgtccc ctggaaaatg ccgagaaaaa ccggcagttt tttatcgaac agacggttat
     2581 gcagcgccgt cacaatgccg gaaatgataa ttgcgccgat aatgctggta tcgagggttt
     2641 taatcccggc catcattgtc agaccgctac ctgccaccgc gtcctgagtg aaatcgacgc
     2701 cgaagtagct tccccaggtc attcccatcg cattgatgaa atagttccag gtcaggaaac
     2761 tcaccatcac cgccagacaa gcacgtccct gcgcttgctt agcaaggcca atgggtaaac
     2821 cgacagcaaa aatcagcggc atattacgga ataccgtcca accgccctct tcaataatgt
     2881 gtacgatttg cgcgaataaa ctgttcggat cggtcagtga ttccccgaca aacatcgggt
     2941 tttgcagcaa gatggcaaga cccaccacaa tcccggcgaa gggaaacagc agcactggcg
     3001 tgaacatcgc gccgccaaag cgttgaattt gactgagcat ttccacatcc tcttttctca
     3061 attctgtaag taggaagcct gattggttat gcggcaggcc tggagcgagg ataggaagat
     3121 gcgatcggca gaacatgacg acccaatggg attcgtgatc gctttcatgc ttttcgttgg
     3181 gcggtagaga tctacgtttt cgcgaaaaag tagacatctg tgagcacagg tacacagcat
     3241 cgaacacatc tttaaaaaaa agattttttt caatcgatta agcagaactt gtgggcgcat
     3301 tacccgtgct tgcaggcaaa aaagagagct agagatgatc tacaaaagca ttgcggagcg
     3361 gttaagaatt cgacttaatt ccgcggattt cacgctaaac agccttcttc ccggtgaaaa
     3421 aaagctggcg gaagagtttg cggtatcgcg gatgactatc cgtaaagcca ttgacctgct
     3481 ggtagcgtgg gggctggtgg tccgccgcca cggcagcggc acttacctgg tgcgcaaaga
     3541 tgtgctgcat caaaccgcca gcctgaccgg actggtggag gtgttaaaac ggcagggaaa
     3601 aacggtcacc agccaggtgc tgatttttga aatcatgcct gcgcctccgg ccattgccag
     3661 ccagttacgg attcaaatca acgagcagat ctacttctcc cgtcgcgttc gttttgtgga
     3721 agggaaaccg ctaatgctgg aagacagcta tatgccggta aaactgttcc gtaacctttc
     3781 gctgcaacat ctggaagggt cgaagtttga atatattgaa caagagtgcg ggattttgat
     3841 tggcggtaat tatgaaagcc tgacgccggt gctcgccgat agactgctgg cgcggcaaat
     3901 gaaggtagcg gaacacacgc cactgctgcg gatcacctcg ttgtcatata gcgagagcgg
     3961 ggagtttttg aattattcag tgatgttcag aaatgccagc gaatactagg tggagtacca
     4021 tttacggcga ctccacccgg aaaagagtta accgatactc cagaagagca ccgccagtaa
     4081 ttggggggtg ataatgcgca ggaacatcac taacggatag acagtggcgt aagagagcgc
     4141 cgccgcacca ctggttggat gaagattatt agcaaacgcc agcgccggag gatcggtcat
     4201 ggaacctgcc agcatcccgc acatggtcag gtaattcatt ttggctaaca tccgcgccag
     4261 aatgccaaca gtaatcagcg gaacggcggt gatcagggca ccataaccaa tccagcttag
     4321 cccttcgcca ttgaccaggg tattcacaaa atccccacca gatttcagac caacgaccga
     4381 gaggaacagc acgatcccca gctcccgcag cgcgaggttg gcacttggcg gcataaacca
     4441 gtacagcttg ccgatactgc cgatacgccc gaggatcaac gccataatca gcggaccgcc
     4501 cgccagcccc agtttcaacg cggccgggaa tcctggcaca aagacgggaa tagaacctaa
     4561 caatacgcca agcccgatgc caataaacac tggcagcatc tgaacctgtt gcagtttttg
     4621 ctgcgcattc cccagcacat tggcaacggc atcaattgcg gacggacgcc ccaccaggtt
     4681 gaggatatcg ccgaactgca ggctgatatc gccactggcg accagttcga ccccggcacg
     4741 gttcaggcgc gagataacaa cgtcatagcg ttctttaaag tgcaggtcgc gaatacgttt
     4801 tccgagcacg ttttcattgg tgaccaccac acgctcgacg cgcaaatcag tgcctttcgt
     4861 agacagcgag gtatcgacct cctgaccaat cacctgttgc gcattatgta aatccgctgg
     4921 ctgccccacc agatgcagca aatcgcccag ttggataatg gtatctggcg aaggtacttt
     4981 tagggtttct tctcgtttca gacgcgagca gataattttg tcgccgttga gaatcggtac
     5041 atctttaatg gctaaatcat gcaggttagg gttctcaacg cgaatattga tagtcttgat
     5101 cagcgcgccg ccattggtgc gtgaagactc gtgctgctga gcttctgtct cgacattgac
     5161 gcggaaaata acccgcaaca tccacatggt gaacaaaatc ccgcaaatgc cgaatggata
     5221 cgccatcgcg taactcatcc ccatctgatc gaccatttcc attggtgtac ccaggtcgcg
     5281 caagatctgc tgccctgccc ccagcgctgg cgtattggtt accgcaccgg agaaaatccc
     5341 cagcactacc ggcagtggaa tatcaaacag tttatgcagg atggcggtaa ccagaccacc
     5401 gatgatgacg atcagaacag caaacaggtt gaggcgtaat ccggagacgc gcaatgaggc
     5461 aaagaagccc ggccctacct gaatcccgat ggtataaacg aacaggatca ggccaaattc
     5521 ctgaataaca tgcagcatat cgctacttaa tgtcatcccc gcctgagaaa caaaatggcc
     5581 gacgatgatc ccaccaaaca gcacgccgcc aatacctaat cctatgccgc gaaatttgac
     5641 gttgccgata aacaaaccga cgactgccac caaagccaga atactgaccg ttaatgctat
     5701 atcactcatc gtccatttct cttgagataa cattttaatt gctacggatt atggctgaat
     5761 cggtgaagca gatttgcctt atagcgcaca ttatcacggg aaagaacgtg ccgaaatatc
     5821 ttaaacagtc gaaatttaac gcattgttga agaagaaaga caaaaaaagc cccaccagaa
     5881 tggcggggca aagagaatag ctagttagct atttaacgcg ggacgttcgc tgatagcgat
     5941 acgctgcgct gcgatgggtt caggctcatt acgaattaaa tcaatatgca gtaaaccgtt
     6001 tacgaaggtt gcgccagaga cttccatatt ttcagccagc gtaaaactca ggctaaatgg
     6061 ctgattcata agcccttgat gcagccattt tttctcttct tttggctgct ccggcgtgcc
     6121 ttttacgctc aggcgcgtac cttccagttg aatctctaaa tcttcctgac ggaaacctgc
     6181 cagcgcaagg gtaatgcggt agtggttatc gtcgcttttc tcaatgttgt acggcgggaa
     6241 gctctgactt tcaccggcgt tttgcagtgc gttggccagt ttgtcaaaac cgatccattg
     6301 acgcatcagt ggggataaat cgaagttacg catagtcatt tctccttcta agaagcgagt
     6361 aagtacctgc aaatccgaag attcgcatat gctccctgac ggcgagcatg gagatgtcag
     6421 gccgcgccag gcggccttag ggaattagtt gatttcgata cggcgcggtt ttttcgcttc
     6481 cggaatcacg cgttcgagat cgatatacag caaaccattt accaggttag ccccacgaac
     6541 atgaatgttc tcagctaact ggaatttgcg ttcaaagttg cgttcagcga tgccctggta
     6601 cagataggtg cgctcttttt gttcgtcggc gtgagcacct ttcaccacca gcagattatc
     6661 ctgggcggta atttccagtt cgctctcagc aaaaccagcc acagcgatag caatgcggta
     6721 atggttttcg tctaccagtt caacgttata cggagggtag ccgccattac tctggctctg
     6781 gttgttttct aagtggttaa acaaacggtc aaatccaata gcagaacggt aaagcgggga
     6841 taaatcaaag ttacgcataa tcaatagctc ctgaaatcag cgagaatgta agaccttcca
     6901 caatggacag gtcaggtagc cagaacaccc atcaggcgcg tcctcatcga ctacgatgta
     6961 aaatgggtct ggaaatgaac ttttcaagcc ttatcagcgg actttttttt gcagtttatg
     7021 gtctattgca tagactgagg gggcagcacg aaacgttaaa gtgcgatagc cgccacagtg
     7081 cgacgtaatg atgatgttat tttcagcaag ggatcgctat aactcatcag gccaacaccc
     7141 acgatcaaca gatgaattta ttatgataag aaatgtgttg ttagcgttca tgatatgcag
     7201 cggaatgaca ttactcggag gatgctccag cgtgatgtcc cataccgggg ggaaagaagg
     7261 aacgtatccc ggcacccgcg ccagcgccac catgattggc gacgatgaaa ccaactgggg
     7321 cactaagtcg ctggcgattc tcgatatgcc gtttaccgct gtgatggaca cgcttttact
     7381 gccgtgggat gtgttccgca aagacagctc ggtgcgctcg cgcgttgaaa aaagcgaagc
     7441 caacgcgcaa gccaccaatg cggttattcc accggccaga atgcctgata actgatttag
     7501 cgatcagttt cggtgatcca cagctggcca ccttccatcc acgccagcca ttgaccatcc
     7561 ggcgagaaga ccacggcgtc cgcagaaggt ggattggcgt ggttttcggt taaatactca
     7621 acctcgccgc tttgcgcatg ggcacaaaca attcgattat ccagcacaaa gcccaaccat
     7681 tctcctgacg gatgccagtt aaatgcagac tgaatatccg ttttgttatg ggttaacttg
     7741 cgcggttcgc cgccctgtgg cgagataagc cacagttgca caatgccgtt atcatcacgc
     7801 attaaaaacg cgatttgcgt accctgcgga ttacagcgca cccagtggcg cgggacgttg
     7861 actaaccccg gataagcccg atgatgggta aaggttaaac gtcgctgcac gacgccacgc
     7921 ggtggcgcgg gtagggttgt ttccgttcca cttaacggcg catcacctgc cgatttccag
     7981 ctagcttcat cttgcggtaa ctcaacgata aatagctccg gcactttctc gccctttggc
     8041 gaaagtgtgt cgccaataaa cgccagcgcg tgatttccta cccatccttc ttcataagca
     8101 cgattgattt catcactgcc aggctgcggc gtgggcgtgg ttttactcac cagcacacac
     8161 cagtggctac cgctgtattc acgcggatgt tgcttttgta cgttgaccgg gccaaacggt
     8221 gcagcaaccc cgacgtttcg caaatccagc gccggatcga gttgatgcat tacatggtcg
     8281 ttataggtaa agctccccct ttcaccgttc gggctaaaga catgcacatg gctgccaccg
     8341 cgcagcgctc ctggcgtata cggcgcggta atatccattg catcgagatt gctcaccttg
     8401 ccgccttcag caatgactcc gcgtcgatga tggaagtcgt aatgccatgt ttcatcagga
     8461 ttttcagggc cgtgaataaa gacatatttc tctgacttcg gatgaacggt caccacgccg
     8521 acgtgtgcgc cctgtgacgc gcgatagata acctcgacct cgcctgtatg gatattcaca
     8581 cgctcaatgg tctcgccggt aaacgacgcc ccagaaggac gcacgtcaaa taccagccac
     8641 tggctgtcgg gcgtccaggt attggtattg gtgagcaggt gattacgggg agcaaaggtt
     8701 atctgtttca tggcgcggtc ctgatacaaa acggttcccg ccatcatact tcacaaggac
     8761 ttcactttca gacaatagtc ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat
     8821 ttcgacgtgg cgattattgg cctcggcccg gcagggtcgg cgttggcacg aaagttagcc
     8881 ggcaaaatgc aggtgatcgc gctggataaa aagcaccagt gcggtactga aggtttcagc
     8941 aaaccttgcg gcggtctgct ggcaccggac gcgcagcgat cttttattcg cgatggactg
     9001 acgcttcctg tcgatgtgat cgccaatcca cagattttca gcgtcaaaac tgtcgacgtc
     9061 gccgcatcgc tcacgcgtaa ctaccagcga agctatatca atattaatcg ccatgctttc
     9121 gacttgtgga tgaaatcgct gatccccgcc agcgttgagg tttatcacga tagcctgtgc
     9181 cgaaaaatct ggcgtgagga tgataaatgg catgtcattt ttcgtgcaga cggctgggag
     9241 caacatatta ccgcccgcta tctggtcggt gccgatggcg caaactcgat ggtgcggcga
     9301 catctctacc cggatcatca aatccgtaaa tatgtcgcta tccagcagtg gttcgcagag
     9361 aaacatccgg tgccgttcta ctcctgcatc tttgataatg cgataactga ctgttactca
     9421 tggagtatca gcaaagatgg ttattttatc tttggcggtg cctatccaat gaaagacggt
     9481 cagacgcgtt tcacgacgct gaaagagaaa atgagcgcct ttcagttcca gtttggcaaa
     9541 gcggtgaaaa gtgagaaatg cacggtgttg tttccctcac gctggcagga ttttgtctgt
     9601 ggtaaggaca acgcctttct gattggtgaa gcggcgggat ttatcagcgc cagctcgctg
     9661 gaggggatta gctatgcgct ggatagcgca gagattctgc gtgcggtgtt cctgaaacag
     9721 ccggagaaga gcgacgccgc ttactggcgc gccacacgca aactgcgttt aaaactcttc
     9781 ggcaagatag taaaaagccg atgcctgacc gcaccggctt taagaaagtg gattatgcgc
     9841 agtggtgtgg cgcatattcc acagttgaaa gattatccaa cgcgcttcac atcacccacc
     9901 agcaggatgt aagagagcgc gccaatcaac gcgacggcgg agatataaac cagtgcaggc
     9961 gcgaaaccgt aaccctgcgc caggtacccc accaccagcg gaacggtgat gccgcccaga
    10021 ccaccggcga agttaaacac gccgccggtt aaaccaatca ggcgcatcgg tgccagagaa
    10081 gagaccagcg accaggtaat cgaagcaaaa ccgttgccga agaatgccag cgccatcagg
    10141 cacataatca tcatcggatc gttggtgtag ttagcgccca taatgcaggt ggagatcagc
    10201 aagccgcaga taatcggcgt tttacgcgca aagcccagtg aaaagccctt acgtaccagc
    10261 agatccgcga cccagccaga gagcaggacg ccgacaaacg ccgcgaggaa tggcaccgtg
    10321 gtcataaagc ctgctttcag cgccgtgatt cctttttcct gggttaaata gttcgggaac
    10381 caggttaaga aaaaccacag tgcagaagcc accgcaaatt gcccaagata aacgccgatc
    10441 agtttacgat ggaacaccag tttccagtct ttggctgtta acggctgacg cgcctctttc
    10501 ttcaccggcg catcaccatc caccagaccg ccgccatcac gaatgtaatc cagttcagct
    10561 ttgctgatac ctttggtcag gcgcggcggc tgataaacct taaaccaaat cagcgaccag
    10621 ataatgccga taccaccagt gacaatgaac acccagtgcc agctcaacat ctcctgaatc
    10681 cagatcagca gcggagtcaa aaacgccaga ccgacaaact gaccagacgt ataaaaacca
    10741 acggcggaag cgcgttcatg ttccgggaac cagctggtca ccatccggtt attggtcggg
    10801 aaagcgggcg cttcgaaaat accggttatc gcacgcagac caattaatga cattaatccc
    10861 gtggcaaagc cctggaataa agtcgccact gaccaaccaa atatcgcaat aaaataagtc
    10921 acgcgagaac ctacgcgatc taaaaaccaa ccgccgggga tctggcatag cgtataaagc
    10981 caagcgaagg ccgaaaatac atagcccatt tccgctttcg taatgccgaa ctcttcctga
    11041 atatgggcgg aagccacggc caggttagcg cggtcaacat aacaaatgac caccgtaata
    11101 aagatcatca ccagcgtcag ataacgccga cgccccggct ttgctgcatt aacgggaata
    11161 tccatagcga gctttctcca gattttgggc atagcgaagc cgctcaccat gccctgtaat
    11221 ttacagaggg ttatattttt gtattgctgt tttagtgccc gatgaggggc ttacgtggca
    11281 ggaattacca ctctgctacg ctgttatctt catgacgcca gagcggatta cgccagtccg
    11341 gggcattttt actgaactca ataactttag cttcgtcgat ttccacacct aagcccggtt
    11401 tcgttaacgg tttaaagaag ccgccaacca tgctgaagtc ttctttgttt ttcacaaagt
    11461 cgagtaactc cgcgcctttg ttgtaatgaa tgcccatact ttgttcctga agtaccgcgt
    11521 tataggaaac aaagtcgata tgcaggcaag ccgccagtgc aatcggtccg aacggacagt
    11581 gcggcgcaag ggtcacatca taggcttctg ccattccagc aattttgtag cattcggtaa
    11641 taccgcctgc atgggagaga tccggttgca gaatcgaaat accgcctgcc tccagcacgc
    11701 gtttaaaatc gaaacgtgag aacatgcgct cacccgccgc cagtggaata tgcgtttgtg
    11761 ccgccaattt cgggtagtat tcggcttgtt ccgccagcac cggctcttca ataaacagcg
    11821 ggcgatacgg ctccagttct ttaatcagca ctttcgccat tggcgcgctg acgcgaccgt
    11881 ggaaatcaag accaaactca atctgattgc caaaagcttc acgaatttgt gccacggtgt
    11941 tgactgccgc atctaccgcg cgggagttat caattagccc cagttcttca caaccgttca
    12001 gtttgaaggt atcgaagccg atttcgcgca gggttttaat gccgtcgata acatccgccg
    12061 gacgatcgcc gccgacccaa ctgtaggctt taattttgtc gcgaaccagg ccgcccatca
    12121 gttgccagac tggcgcattc agcactttgc ctttgatatc ccataacgcc tggtcgatcc
    12181 cggcgatggc gctcatcagg attggaccgc cacgataaaa tccggcgcga tacatcactt
    12241 gccataagtc gttgatgcgc gaaggatcct gaccaatcaa atagtcaccc agctcgtgaa
    12301 ctgccgcttc caccgtacgg gcgcggcctt caatcacggg ctcgccccaa ccgaccacgc
    12361 cttcatcggt ttcaattttc aggaacatcc agcggggagg taaacgatac gtggtaattt
    12421 tggtgatttt cattgcactg cctctcgata cgccttaaca aatgctgctg cctgctgcgc
    12481 ggtgcgttct acggactgcc cggcgcgata gagatcgctg cctaagcccg ccccaacaca
    12541 acctgcgtct atccactgcg ccaggttttc tggcgtcacg ccgccaacgg caaagactgc
    12601 gatgtccggt ggtaataccg cttttaacgc tttgatgtat tgcggaccaa aagccgatga
    12661 cggaaatatt ttcagcgcct gcgcgcccgc ttcgagcgcg gtaaaggctt cggtcgccgt
    12721 cgcgcagccg gggcaaacgg tcatgccgta gcccaccgcg cggcggatca cttcactatg
    12781 gatattgggc gtaacgatga gctgacagcc catcctggcg agcgcatcga cctgttcagg
    12841 tttcagtacc gtacctgcgc caatcaacgc cttgtcgcca tacgcatcaa cgatggcggg
    12901 aatgctctgc tcccattgtg gggaattcag cgggatttca accgcgtcga acccggcgtc
    12961 aatcaccgcg ccaacatgcg ccagcgcctc gtcgggcgta atgccgcgca aaatggcgat
    13021 cagcgggagt ttagtttgcc actgcatgag cgatgctcct tataccagcc tgaaatgccg
    13081 tgtcgcccgc caccgccgcc acgtcgcaac ccatcgcctg aaaggcttgc tggtagcgcg
    13141 cggtcagcga tgttccggcg acaagggtga tggcgtgttg atgggtcaca tagtcgcgca
    13201 tactggcaac ctctgcgcca atcaacaaac cagagagaaa ttcgctgacc tgttcgcggg
    13261 gaagtgttcc cagcacatgc gaggcgcgaa cttcaaaaag ctgcggcaat atggcgggcg
    13321 cattaaggcc gcgctcaagg ccagccgcga aggcatcggc agagttttcc tgcggcggca
    13381 aacctgcgcc aatcaatgag tgatttaaca gtaaatgatg taattcaccg gtcatcacgg
    13441 tgcgaaaatc gttgatttgc tggctatcgg cctgcaccca tttgcaatgc gtaccgggca
    13501 taacataaag agaggaagga gccagagctc gcgcgccgat caattgtgtt tcttcgccgc
    13561 gcatcacatt gtggttatcg tcatgagaga cgcataatcc gggaataatc cagatattgt
    13621 cgccaacaga cgttaattgt tcgccaatag acgaaaaacg ggcaggaaca gataaatacg
    13681 gagcaacttt ccagccgaca ttgctgccga ccattcctgc catcaccact ggcgtatttt
    13741 cttcacgcca gtcggtcgtg acttctgcta acaccgcagc cggagatttt ccgttcaggc
    13801 gcgtgacgcc tgcttctgat tgcctgctct ccaggcagtg gtcgccctga taaagccagg
    13861 cgcgcagatt ggtcgatccc cagtcaattg cgatgtagcg agctgtcatg tgatttcctt
    13921 taaccttcgt gtcgagctgg caatcatggt aagcgccgcc tgctctgccg catcgccgtc
    13981 ctgatgccgt atcgcatcga acagcgcctt atgttcctgg agcgtttgcg gcatgttggc
    14041 ctcatcgccc atccaggttc gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct
    14101 aagttgctgt aacaccgggt tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc
    14161 cgcttcgtta aacgcttcgc ggtcctgatt gttggcaatc atctcgttca gcgccgattc
    14221 aatctgcgcc agatcgctgg aggtcgcgcg ctctgctgcc caacgggcaa tcgccggttc
    14281 caccagattt cgcacttcgc tcatggcact gataagccgt gggtcgtagt cgttttccag
    14341 cacccattgc agtacgtcag tgtcgaggta attccactgg ttacgcggtg ccacaaacgc
    14401 cccgcgataa cgtttcattt caatcagccg cttcgccatc agcgaacgga acacctcacg
    14461 gatgatgttg cgcgaggttg caaactcttc acagagctcc gcctcagccg gaagtggcga
    14521 gccaggtacg tatttgccgt gaacgatctg tttacccagc gtaatgacaa tgcgatcggt
    14581 tttattgaga gtcatggaga gtccttgtgc tctgtatgtt cttctctact ttaccccgat
    14641 cgatgcataa cgcggcaact ttgtagtacc agcgtgatga cgttcgcgtt tgccgtgtgt
    14701 gtaatgtggt acaaacttat gttgttgtac tacaatttag atcaaaaaaa gaacaatgcg
    14761 taaaaaaatg acatgcgtcg ggcagaaatc tgaaaaggga tatcaggcgc taaacaggag
    14821 ggaaagaaga gtatgctttc aacggcttag ctactcgttt aaaggattaa tcatgaagtt
    14881 gaattttaag ggatttttta aggctgccgg tttattccca ctggcgctga tgctttcagg
    14941 ctgtatctcg tatgctctgg tttcccatac cgcaaagggt agttcaggaa agtatcaatc
    15001 gcagtcagac accatcactg ggctatcgca ggcaaaagat agtaatggaa caaaaggcta
    15061 tgtttttgta ggggaatcgc tggattacct tatcactgat ggtgccgatg acatcgttaa
    15121 gatgctcaat gatccagcac ttaaccggca caatattcag gttgccgatg acgcaagatt
    15181 tgttttaaat gcggggaaaa agaaatttac cggcacaata tcgctttact actactggaa
    15241 taacgaagaa gaaaaggcac tggcaacgca ttatggtttt gcctgtggtg ttcaacactg
    15301 taccaggtca ctggaaaacc taaaaggcac aatccatgag aaaaataaaa acatggatta
    15361 ctcaaaggtg atggcgttct accatccatt taaagtgcga ttttatgaat actattcacc
    15421 cagaggcatt ccggatggtg tttccgcagc attactgcca gtgactgtta cgctggacat
    15481 cattactgca ccgctgcaat ttctggttgt atatgcagta aaccaataat cagtaagcgg
    15541 gcaaacgcgt ttatgctgtt tgcccgccca cagattaatt cagcacatac ttctcaatag
    15601 caaacgccac gccatcttca atggttggat tttgtgacaa agttcgccac ttctttcacc
    15661 gaaggaatag cgttatccat cgccacaccg acgcctgcat attcaatcat tgcgatgtcg
    15721 ttttcctgat cgccaatcgc catgatttct tccggtttaa tacctaacac gtcggccagt
    15781 gatttcacac ccgtaccttt gttaacgcgt ttatcgagga tttcgaggaa gtacggcgca
    15841 cttttcagca cggtatattt ctctttcact tcctgcggaa tacgcgcgat agcctggtcg
    15901 aggatggcgg gttcatcaat catcatcact ttcaggaact gggtattggg gtccattttc
    15961 tccgcttcgc agaacaccag cggaattgtg gcaacgaagg attcatgcac cgtgtagtag
    16021 ctgatatcgc ggttagcggt gtacagcgtg gtgcggtcca gggcgtggaa atgagaaccg
    16081 acttcgcgag agagtttttc caggaaacga tagtcgtcat agctgagagc ggtttgcgcc
    16141 acggtgctac catcagcggc cttctgtacc agcgcgccgt tataagtaat gcagtagtcg
    16201 cccggctgtt ccatatgcag ctctttcagg tagttgtgca cacctgcata cggacgaccc
    16261 gtcgttagca cgacattcac gccacgggcg cgagctgcgg caatcgcatt tttaacggcg
    16321 ggtgaaatgg tgtgatcggg cagcagaagg gtgccatcca tatcgatagc aatgagtttg
    16381 atagccatga gttcccaagg tagattagtt cctgacccat gctaacgcga ttccgctcaa
    16441 aaatcagtac aacacccgag ggaaaagggg atgcaacgcg ggttgcatgc tccccatttg
    16501 cttagcggaa gagtttccct ttcagcagtt ccatgcctgc ggaaagcaga tcgttattgg
    16561 cttgtgctga cacttcacct tgcggtgaga gcgcatcaat aatcttcggc aattgctctg
    16621 ccagtaaact ggaagccgta ctggtatcca cgccaagttt ttgcccgaga tcggacaccg
    16681 catttgtgcc gagcgccgat tccagttgct cgccgctaac tgattgattt ctctgttgat
    16741 tactcaacca ggttgagaga atggccccta agccgccact ttgcagtttt tccagcagca
    16801 cctgaatgcc accctgctcc tcaacccaac ttaaaatcgc ctgatatttc cccgcatcgc
    16861 ctttcagaaa ggcaccgaca acttcatcaa aaagccccat gataatcacc tgtaaggcgt
    16921 tacgtgttga cccaaaaagt atagatctgc gggatgataa ttgcggattg cagaaataaa
    16981 aagggcggag atgatctccg cccttttctt atagcttctt gccggatgcg gcgtgaacgc
    17041 cttatccggc ctacaaaatc aatatattgc aggagtctcg taggactaat aagcgtagcg
    17101 catcaggcac gctcgcatgg ttaacgccat taaatatcaa tattcgccgc tttcagggcg
    17161 ttctcttcaa taaacgcacg acgcggttct acggcgtcgc ccatcagcgt ggtgaacaac
    17221 tggtcagcgg caatcgcatc cttaacggta acgcgcagca tacgacggct ttccgggtcc
    17281 atggtggttt cccacagctg ttccgggttc atctcgccca gacctttata acgctggatg
    17341 gagaggccac gacgggactc tttcaccagc cagtccagcg cctgctcgaa gctggctacc
    17401 ggctgacgac gctcgccacg ttcgataaac gcatcttctt ccagcaagcc acgcagtttc
    17461 tcacccagcg tgcagatacg acgatattcg ccaccggtga taaactcgtg atccagcgga
    17521 tagtcagtat ccacaccgtg ggtacgcacg cgaacaatcg gctcgaacag gttttgttcg
    17581 gcattggtgt ggacatcaaa cttccactgg ctgccgtgct gttctttgtc gttcagttcg
    17641 ctgaccagcg cgttcaccca gcgggtaacg gtctgctcat cagaaaggtc agcttccgtc
    17701 aacgtcggct gatagataag ctctttcagc attgctttcg gataacgacg ctccatgcgg
    17761 ttgatcattt tctgcgtcgc gttgtactca gataccagtt tctctaacgc ttcgccagcc
    17821 aatgccggtg cactggcgtt ggtgtgcagc gttgcgccgt ccagcgcgat agagatctgg
    17881 tactgatcca tcgcttcgtc gtctttaatg tactgttcct gcttgccttt cttcactttg
    17941 tacagcggcg gctgagcgat gtagacgtga ccgcgttcaa cgatttccgg catctgacga
    18001 tagaagaagg tcaacagcag cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg
    18061 atgatgatgc tgtgataacg tagtttgtcc gggttgtact cgtcacgacc gataccacag
    18121 ccaagcgcgg tgataagcgt cgccacttcc tgagaagaga gcatcttatc gaagcgcgct
    18181 ttctcgacgt taaggatttt acccttcagc ggcagaatcg cttggttctt gcggttacgc
    18241 ccctgcttcg cagagccgcc cgcggagtcc ccttccacca ggtacagttc ggaaagcgcc
    18301 ggatcgcgtt cctggcagtc tgccagtttg cccggcaggc cagctaagtc gagcgcacct
    18361 ttacggcggg tcatttcacg cgcgcgacgc gccgcttcac gggcacgggc agcatcgata
    18421 attttgccaa ccacgatttt cgcatcagtt gggttttcca gcaggtattc cgccagcagt
    18481 tcgttcatct gctgttcaac cgccgatttc acctcagaag aaaccagttt gtctttggtc
    18541 tgggaggaga atttcgggtc cggcactttt acggaaacga ccgcaatcag gccttcacgc
    18601 gcatcgtcac cggtggcgct gactttggct tttttgctgt agccttcttt gtccatgtag
    18661 gcattcaggg tacgggtcat cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc
    18721 tgcggaatgt tgttggtaaa gcagtagatg ttttcctgga agccatcgtt ccactgcagc
    18781 gccacttcaa cgccaatacc gtctttttcg gtggagaagt agaagatatt tgggtggatc
    18841 ggcgttttgt tcttgttcag atattcaacg aacgctttga tgccgccttc atagtggaag
    18901 tggtcttctt tgccgtcacg cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac
    18961 gacaactcac gcagacgttt cgccagaatt tcatattcga actcggtcac attggtgaag
    19021 gtttcgaggc tgggccagaa acgcaccatg gtgccggttt tttcagtctc gccggtaacc
    19081 gccagcggag cctgcggtac accgtgttcg tagatctgac ggtgaatttt accctcgcgc
    19141 tgaataacca gctccagttt ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc
    19201 agaccgccgg acactttata ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc
    19261 atgatcactt ccgccgccga tacgccctct tccgggtgaa taccggtcgg aatgccgcgc
    19321 ccgtcatcct gtacagagac agagttatcg gcgtgaatgg tgacgataat ttctttacag
    19381 tgacccgcga gcgcttcgtc gatagcgtta tctaccacct cgaataccat gtggtgcaga
    19441 ccggtgccgt catccgtgtc gccgatatac atacccgggc gcttacgcac cgcatccagc
    19501 cctttcagga ctttgatact ggaggagtca taagaattcg acatcaacgt ttctcgctca
    19561 tttatacttg ggttaatccg ttattttacc cttttccacg gtaaacatct tcgaattttc
    19621 gtccgacatg tctataacgt gttcagcact gatcgcgctg acaaagacct gtgattgcgt
    19681 cgcttttaag cggctggcaa gcaacccgcg acgctcatca tcaagctcag aggcaaaatc
    19741 atctatcagg tagagacacc gccgcccgct ttcacgagtg aggaactctc cttgcgccag
    19801 acgtaaggcg cacatcaaca gcttaagctg cccacgcgat aaggtatctt ccaccggcgc
    19861 accgtcggcg cgaatgcgta agtccgcttt atgcgggcca tgcgcggtgt aggttagctg
    19921 gcgatcgcgt tcaaaattac gttccagcac ctcagcgtac tctgtctctt tctcccagcc
    19981 gcgctggaaa gagaaagtca gagaaaactc agggagaaat tgcttacagg tatcagccat
    20041 atcggccgcg ataccggcgc tatactccgc gcgccaggtg ctgatttgct ccgccagcgg
    20101 gatcagttct ttatcccacg ggcgtagctg ttcgtaacga gtcacctggc gcagcgccgc
    20161 attgcgctgc ttgagcaatc gcttgagatt gctccaggcg gtgaaaaatt caggttcgtt
    20221 gtgaaagcat ccccagtcga ggaatgctct tctgtatttg gggccgccgt tgagtaaagt
    20281 aaacccttct ggcgttatca actgcatcgg catcaggtgc gccagttccg cgactttatg
    20341 cccgtccgta ccgtcaatac ggactttgct gtcgccctgt ttgtctttgg ttaagccaat
    20401 cgccgtctcg cgctcttcgc cctgtaatcg cccgtggaga acaaatgcct cctgctcatg
    20461 gcgaatgacg cgaccaatct gcaaactgcg aaacgcccga ccatggccga gcgtatagat
    20521 ggcttccagc acgctggttt tgccactgcc gttggcacct accagaaagt taaagccggg
    20581 agataaagcg agatccgcgg tttcaatatt gcggaaatcg cggatcaaca agcgggtgag
    20641 ggacattaca gtctcattgg catgacaaca taggcagccg actgtgatgc ggcatcttca
    20701 atctgcacgc tggaaaccga atcggtcagc atcatgcgga cgttttcgca tttcagcgcg
    20761 ttcagaacat ccagcacata gctgacgttg aagccgattt ccatctccgc accgctatag
    20821 gtaacgtcga ggatctcttc cgcttcttcc tgttccgggt tgttggcagt aattttcagc
    20881 tggttttcgc tgacatacag gcgcacaccg cggaatttct cgttagagag aatcgccgcg
    20941 cgagcaaacg cctgcttgag cagatcgcag cctgcttcca gatgtttgtc cgggttcttc
    21001 ggcagaacgc gacggtaatc cgggaagcga ccatccacca gtttcgaggt gaaaataaag
    21061 tcgccaacgt gggcacgaat gttattgctg ccaatctgca cacgcagcag attgtcgccg
    21121 ccgtcgagca tacgcatcag ttcaatcacg cctttacgcg gtacgatcac cgaatggctt
    21181 ggcaaagatt gaccaattgg cattgaacag accgccagac ggtggccgtc ggttgccacg
    21241 gtgcgcagtt cttcaccttc ggtttcaaac agcataccgt ttaagtaata gcgaacatcc
    21301 tgatgcgcca tcgaaaactg ggttgcttca atcagacgct tcatcgtcgc ctgcggcagg
    21361 gtaaattcga cttcgctctg ccagtcatcg aggttcggga aatccgccgc tggcagggta
    21421 gacagcgaaa aacggctacg cccggagcgc accagcatcc gttcaccttc cagctgcacg
    21481 gcaatttccg cgccttcagg cagaccacgg cagatatcaa agaatttgcg cgccggaacg
    21541 gtcgtcgctc ctggctcgtg tggctgaacc agcgcaacgc gtgccaccat ttccatctcg
    21601 agatcagtac cggtcagcga caacgtaccg tcagcaacct gtaacagcag attaccgaga
    21661 atcggtagcg taggacgacc acctaacgga ccgctcacct gttgtagcgg ttttaataaa
    21721 tgctcacgtt ctacggtaaa tttcataggt ttacgatgac aatgttctga ttaaatttga
    21781 aaaatcttct ttgatatcgt ggctctcttc acgcaactgc tcgatcttac ggcaggcatg
    21841 aagcaccgtc gtgtggtcac gcccaccaaa cgcatcgcca atctctggca gactgtggtt
    21901 agtcagctct ttcgccagcg ccatcgccat ctggcgcgga cgcgccaccg agcgggatcg
    21961 acgcttggaa aggagatccg cgactttgat cttgtagtac tccgccaccg tcttctgaat
    22021 attgtcgatg gtgaccagtt tttcctgcaa tgccagcaag tcgcgcagcg cctcacgcac
    22081 gaagtcgatg gtgatcgccc gtccggtaaa gttggcgtta gcaataacgc ggttcagcgc
    22141 cccttccagc tcacgtacgt tagatcgtag acgcttggcg ataaagaacg ccacttcacc
    22201 cggcaaacga atgtcatttt cgtcggcctt tttcatcagg atcgccacac gggtttccag
    22261 ctctggcggt tcgatcgcca cagtcagtcc ccaaccgaag cgggatttca aacgatcctc
    22321 aacgccgttg atctctttcg gatagcgatc cgaggtgaga atgatctgtt gattaccttc
    22381 cagcagggcg ttgaaggtgt ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa
    22441 ctgaatatcg tcgatcagca gtgcatctac ggaacggtag tagcgtttaa actcttcgat
    22501 cgcgttgttt tgcagggctt taaccatgtc ctgaacaaag cgctcggagt gcatataaac
    22561 cactttggca ttcggcttgc gcgccataat gccgttaccc accgcatgca gcaggtgagt
    22621 tttacccaga cccgtgccgc cataaaggaa caacgggtta taggcaccgc cagggttatc
    22681 cgccacctgg cgagccgccg cgcgcgccag ttggttagat ttaccttcaa cgaagttatc
    22741 aaacgtgtgt ttgacgttta cgttagaacg ataggtcggt tctgccgggg ccgggacgtt
    22801 atcccaacct gagcgcgtag aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc
    22861 aggggccgcg acgttgctcg tcactgccgc ttgtggcgtt tgcgtcaccg gtttggtgcc
    22921 gacttcaaaa cgcagctgtg gggcatccgc tccgcagaaa ctggttagca gtccattgat
    22981 attattaagg tacttgtccc gtacccaatc gaggacaaaa cggtttggcg cgtacagggc
    23041 cagcgtgtta tcgctcagtt ccgcctgcaa tgggcgtatc cacatactga attctgtggc
    23101 tggtaactca tcctgcaatc gggcaagaca ctgctgccaa agcgaaagtg acacggcgga
    23161 ctccactcga acaaaagtcg ataatgacta aggctgaaac attcatgatt gttgacgtac
    23221 gtcgaaaaga ccctgcttga gggtgacgca cgaaccgctg tctgcggtta tatgcccgat
    23281 caagatcctg caaaacgctc gggaccgcgg atcatagcct aaactgcgca agagatcttc
    23341 tgtttctcac agattttctc tatttatcca caggactttc cagaactcgc taagtgtaaa
    23401 cgatcctgcc gcgaggcggg cacgatttac gccgcatatt ggaaaattta atgaccatag
    23461 acaaaaattg gcttaattga tctaataaag atccaggacg atccttgcgc tttacccatc
    23521 agcccgtata atcctccacc cggcgcgcca tgctggtttc cactggtgtg aggtcgtaca
    23581 ttttccctgc gaaaaggtgc ggaaaagcgc ggtaaataag gaaagagaat tgactccggt
    23641 gtgtacaatt attacaatcc ggcctcttta atcacccatg gcttcggtgt ccatcgtttc
    23701 atttttcggc ggatatccaa taaagccatt gaatttattc aagtttaggt agaaatcgcc
    23761 atgaaacgca cttttcaacc gtctgtactg aagcgcaacc gttctcacgg cttccgtgct
    23821 cgtatggcta ctaaaaatgg tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct
    23881 cgtctgaccg tttctaagta ataaagctaa cccctgagtg gttaagctcg catttcccag
    23941 ggagttacgc ttgttaactc ccagtcaatt cacattcgtc ttccagcagc cacaacgggc
    24001 tggcacgccg caaattacca ttctcggccg cctgaattcg ctggggcatc cccgtatcgg
    24061 tcttacagtc gccaagaaaa acgttcgacg cgcccatgaa cgcaatcgga ttaaacgtct
    24121 gacgcgtgaa agcttccgtc tgcgccaaca tgaactcccg gctatggatt tcgtggtggt
    24181 ggcgaaaaaa ggggttgccg acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt
    24241 atggcgccgc cactgtcgcc tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa
    24301 cgcctgatta gtccgctact cgggccgcat tgtcgtttca ctccaacctg ttcaagctac
    24361 ggaattgagg cattgcgcag gtttggagtg ataaaaggca gttggttgac ggtgaaacgc
    24421 gtattaaaat gccacccttt acaccctggt ggtgacgatc ccgtcccgcc cggaccattt
    24481 gataccagag aacactaacg atggattcgc aacgcaatct tttagtcatc gctttgctgt
    24541 tcgtgtcttt catgatctgg caagcctggg agcaggataa aaacccgcaa cctcaggccc
    24601 aacagaccac gcagacaacg accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg
    24661 ccagtggcca ggggaaactg atctcggtta agaccgacgt gcttgatctg accatcaaca
    24721 cccgtggtgg tgatgttgag caagctctgc tgcctgctta cccgaaagag ctgaactcta
    24781 cccagccgtt ccagctgttg gaaacttcac cgcagtttat ttatcaggca cagagcggtc
    24841 tgaccggtcg tgatggcccg gataacccgg ctaacggccc gcgtccgctg tataacgttg
    24901 aaaaagacgc ttatgtgctg gctgaaggtc aaaacgaact gcaggtgccg atgacgtata
    24961 ccgacgcggc aggcaacacg tttaccaaaa cgtttgtcct gaaacgtggt gattacgctg
    25021 tcaacgtcaa ctacaacgtg cagaacgctg gcgagaaacc gctggaaatc tcctcgtttg
    25081 gtcagttgaa gcaatccatc actctgccac cgcatctcga taccggaagc agcaacttcg
    25141 cactgcacac cttccgtggc gcggcgtact ccacgcctga cgagaagtat gagaaataca
    25201 agttcgatac cattgccgat aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg
    25261 cgatgctgca acagtatttc gcgacggcgt ggatcccgca taacgacggt accaacaact
    25321 tctataccgc taatctgggt aacggcatcg ccgctatcgg ctataaatct cagccggtac
    25381 tggttcagcc tggtcagact ggcgcgatga acagcaccct gtgggttggc ccggaaatcc
    25441 aggacaaaat ggcagctgtt gctccgcacc tagatctgac cgttgattac ggttggttgt
    25501 ggttcatctc tcagccgctg ttcaaactgc tgaaatggat ccatagcttt gtgggtaact
    25561 ggggcttctc cattatcatc atcaccttta tcgttcgtgg catcatgtac ccgctgacca
    25621 aagcgcagta cacctccatg gcgaagatgc gtatgctgca gccgaagatt caggcaatgc
    25681 gtgagcgtct gggcgatgac aaacagcgta tcagtcagga aatgatggcg ctgtacaaag
    25741 ctgagaaggt taacccgctg ggcggctgct tcccgctgct gatccagatg ccaatcttcc
    25801 tggcgttgta ctacatgctg atgggttccg ttgaactgcg tcaggcaccg tttgcactgt
    25861 ggatccacga cctgtcggca caggacccgt actacatcct gccgatcctg atgggcgtaa
    25921 cgatgttctt cattcagaag atgtcgccga ccactgtgac cgacccgatg cagcagaaga
    25981 tcatgacctt tatgccggtc atcttcaccg tgttcttcct gtggttcccg tcaggtctgg
    26041 tactgtacta tatcgtcagc aacctggtaa ccattattca gcagcagctg atttaccgtg
    26101 gtctggaaaa acgtggcctg catagccgcg agaagaaaaa atcctgattc ggtgagtttt
    26161 cgctaaaata agggcggtca gttgaccgcc ttttttcttt tcgtagggcg gataagcacc
    26221 gcgcatccgc cacacaaagc aacaggaaca tcatgagcga taatgacact atcgtagccc
    26281 aggccacgcc tccgggacgt ggcggcgttg gcatcctgcg catctccggc ttcaaagccc
    26341 gtgaagttgc cgaaaccgtg ctgggtaaac tgcctaagcc gcgctacgcc gattatcttc
    26401 cgtttaaaga cgccgacggc agcgtgctcg atcaggggat tgcgctatgg ttccctggcc
    26461 cgaactcgtt caccggcgaa gatgtgctgg aactgcaagg tcatggcggt ccggtgatcc
    26521 tcgacctgct gttaaaacgc attctgacca ttcccggcct gcggattgct cgccctggtg
    26581 agttttccga acgcgcgttt cttaacgata aacttgactt agcccaggcc gaggcgattg
    26641 ccgatcttat cgacgccagt tcggaacagg cggcccgttc ggcacttaac tcgctgcaag
    26701 gcgcattctc cgcacgggtt aatcatctgg tagaagccct cacccacttg cgcatttacg
    26761 tcgaagcggc aattgatttc cctgatgaag agatcgattt cctctccgac ggcaaaattg
    26821 aagcccagct taatgacgtg atggccgatc tcgatgcagt gcgtgctgaa gcatgtcagg
    26881 gtagtttgtt gcgcgaaggg atgaaagtag tgattgccgg acgtcctaat gccggtaaat
    26941 cgagcctgtt aaacgcgctg gcgggccgtg aagcggcaat cgtaaccgat atcgccggaa
    27001 ccacgcgtga cgtgctgcgt gagcatatcc acattgacgg aatgccgctg catatcatcg
    27061 acactgccgg gctacgtgag gccagtgacg aagtggaacg tattggtatc gagcgcgcgt
    27121 ggcaggaaat tgaacaggcc gaccgcgtgc tgtttatggt cgatggcacc acgacagacg
    27181 ccgttgatcc ggcagagatc tggccggaat ttatcgcccg tctgcccgcg aaactgccga
    27241 tcaccgtggt acgcaataaa gccgatatca ccggcgaaac gctgggaatg agtgaagtga
    27301 acggtcacgc gttaattcgt ctctcggcaa ggactggtga aggcgtggac gtgctgcgta
    27361 accatctcaa acagagcatg ggctttgaca ccaatatgga aggcggcttc ctggcgcgcc
    27421 gtcgccactt acaggcgctg gaacaggcag cggagcattt gcagcaaggt aaagcgcaac
    27481 tgttgggagc ctgggcaggt gaactgctgg cggaagagtt gcgcctggca cagcagaact
    27541 taagcgaaat caccggggaa tttacttcag acgacctgct ggggcggatt ttctccagct
    27601 tctgtattgg taagtaaccg cgcttacgaa gccgtattct gactgtcaga tgcggcttcg
    27661 cttcattgtt agcactcctg ttatgcctca acccttttt
//
LOCUS       DADLSA010000040        26913 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.040, whole genome shotgun sequence.
ACCESSION   DADLSA010000040 DADLSA010000000
VERSION     DADLSA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.040"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            126..449
                     /gene="iraM"
                     /locus_tag="JN691_001736"
     CDS             126..449
                     /gene="iraM"
                     /locus_tag="JN691_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY7243198.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIQIC
                     PYGLK"
     gene            complement(552..1802)
                     /gene="icd"
                     /locus_tag="JN691_001737"
     CDS             complement(552..1802)
                     /gene="icd"
                     /locus_tag="JN691_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAY7243199.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1974..2627
                     /gene="rluE"
                     /locus_tag="JN691_001738"
     CDS             1974..2627
                     /gene="rluE"
                     /locus_tag="JN691_001738"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAY7243200.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTKRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGTELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2637..3098
                     /gene="nudJ"
                     /locus_tag="JN691_001739"
     CDS             2637..3098
                     /gene="nudJ"
                     /locus_tag="JN691_001739"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAY7243201.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3152..4258
                     /gene="mnmA"
                     /locus_tag="JN691_001740"
     CDS             3152..4258
                     /gene="mnmA"
                     /locus_tag="JN691_001740"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAY7243202.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4294..4935
                     /gene="hflD"
                     /locus_tag="JN691_001741"
     CDS             4294..4935
                     /gene="hflD"
                     /locus_tag="JN691_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAY7243203.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4939..6309
                     /gene="purB"
                     /locus_tag="JN691_001742"
     CDS             4939..6309
                     /gene="purB"
                     /locus_tag="JN691_001742"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAY7243204.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKNIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6478..7149
                     /gene="phoP"
                     /locus_tag="JN691_001743"
     CDS             6478..7149
                     /gene="phoP"
                     /locus_tag="JN691_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAY7243205.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            7149..8609
                     /gene="phoQ"
                     /locus_tag="JN691_001744"
     CDS             7149..8609
                     /gene="phoQ"
                     /locus_tag="JN691_001744"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAY7243206.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAEESMLGGARMEVIFGRQHSAPKDE"
     gene            8685..9806
                     /locus_tag="JN691_001745"
     CDS             8685..9806
                     /locus_tag="JN691_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY7243207.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9855..11081)
                     /gene="pepT"
                     /locus_tag="JN691_001746"
     CDS             complement(9855..11081)
                     /gene="pepT"
                     /locus_tag="JN691_001746"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAY7243208.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEALSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            11331..12467
                     /gene="potA"
                     /locus_tag="JN691_001747"
     CDS             11331..12467
                     /gene="potA"
                     /locus_tag="JN691_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAY7243209.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12451..13308
                     /gene="potB"
                     /locus_tag="JN691_001748"
     CDS             12451..13308
                     /gene="potB"
                     /locus_tag="JN691_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAY7243210.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            13305..14099
                     /gene="potC"
                     /locus_tag="JN691_001749"
     CDS             13305..14099
                     /gene="potC"
                     /locus_tag="JN691_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAY7243211.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            14096..15142
                     /gene="potD"
                     /locus_tag="JN691_001750"
     CDS             14096..15142
                     /gene="potD"
                     /locus_tag="JN691_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAY7243212.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(15298..16119)
                     /gene="cobB"
                     /locus_tag="JN691_001751"
     CDS             complement(15298..16119)
                     /gene="cobB"
                     /locus_tag="JN691_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAY7243213.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(16135..17046)
                     /gene="nagK"
                     /locus_tag="JN691_001752"
     CDS             complement(16135..17046)
                     /gene="nagK"
                     /locus_tag="JN691_001752"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAY7243214.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17075..18319)
                     /gene="lolE"
                     /locus_tag="JN691_001753"
     CDS             complement(17075..18319)
                     /gene="lolE"
                     /locus_tag="JN691_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAY7243215.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(18319..19020)
                     /gene="lolD"
                     /locus_tag="JN691_001754"
     CDS             complement(18319..19020)
                     /gene="lolD"
                     /locus_tag="JN691_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAY7243216.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(19013..20212)
                     /gene="lolC"
                     /locus_tag="JN691_001755"
     CDS             complement(19013..20212)
                     /gene="lolC"
                     /locus_tag="JN691_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAY7243217.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPVQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            20474..21547
                     /locus_tag="JN691_001756"
     CDS             20474..21547
                     /locus_tag="JN691_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY7243218.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21675..25121
                     /gene="mfd"
                     /locus_tag="JN691_001757"
     CDS             21675..25121
                     /gene="mfd"
                     /locus_tag="JN691_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAY7243219.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            25264..26226
                     /gene="ldtC"
                     /locus_tag="JN691_001758"
     CDS             25264..26226
                     /gene="ldtC"
                     /locus_tag="JN691_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HAY7243220.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPIGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(26309..26566)
                     /gene="bhsA"
                     /locus_tag="JN691_001759"
     CDS             complement(26309..26566)
                     /gene="bhsA"
                     /locus_tag="JN691_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAY7243221.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            26808..>26913
                     /locus_tag="JN691_001760"
     CDS             26808..>26913
                     /locus_tag="JN691_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7243222.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQH"
BASE COUNT         6621 a         6946 c         6793 g         6553 t
ORIGIN      
        1 tttgttttat ataagcaatc gttttaatta atttattttt tgaaggggta atatactcat
       61 atgcaaaata aagaaatgaa catccaaatg aactatatta aataccgtgg gataagacat
      121 aacaaatgaa gtgggtagta attgacacgg taattcaacc tacatgcggc atatcttttt
      181 cagccatatg gggtgatatg aaaatgatca tctggtatca atctactata tttcttcctc
      241 ctggaaggat atttacaccg gttaagcttg gtattatcct taaggaaaaa gaatatccta
      301 ttactattta taacattgca ccattcaaca agaatttatg gagtttactc aaaagcagtc
      361 aagagtgtcc tccaggagaa agcgaaataa caaataaatg tttgcataag agttgtatta
      421 tacaaatatg cccatatggg ctcaagtaat aggttttata taaatatgct cgatatgcta
      481 ttaataatat ccacataaat aaaacaacgg gcgtgttata cgcccgttta aatatttaac
      541 acatgtagtg attacatatt cttgatgatc gcatcaccaa actctgaaca tttcagcagt
      601 ttagcgcctt ccatcagacg ctcgaagtca taggttacgg tcttggcgtt gatcgcgcct
      661 tccatacctt taacaatcag gtctgcggct tcagtccagc ccatatggcg taacatcatc
      721 tctgcggaga gaataataga gccagggttt actttgtcct gaccggcgta tttcggcgca
      781 gtaccgtggg tggcttcaaa cagggcgcat tcgtcaccga tgtttgctcc aggggcgata
      841 ccgataccgc caacctgcgc tgccagagcg tcagaaatgt agtcaccgtt caggttcata
      901 caggcgataa catcatattc agccggacgc agcaggatct gttgcaggaa tgcatcagca
      961 atcacgtctt taatgacgat ctctttgccg gtattcgggt ttttaacttt cagccacggg
     1021 ccgccgtcga tcagttcacc gccaaattct tcacgcgcca gctggtagcc ccagtcttta
     1081 aacgcgcctt cggtgaactt catgatgttg cctttgtgca ccagggtcac agagtcacga
     1141 tcgttagcaa ttgcgtattc gatcgctgca cgaaccagac gtttggtgcc ttcttcagaa
     1201 cacggcttaa taccgatacc gcaatgttcc gggaagcgaa ttttcttcac gcccatctct
     1261 tcacgcagga atttaatcac tttctcggcg tcggcagagt cagctttcca ttcgataccc
     1321 gcataaatgt cttccgagtt ttcacggaag ataaccatat cggtcagttc agggtgttta
     1381 accgggcttg gagtgccctg atagtaacgt accggacgca ggcagatgta gagatccagt
     1441 tcctgacgca gggcaacgtt gagagaacga ataccgccac caaccggagt ggtcagcggg
     1501 cctttaatgg caacgcgata ttcacgaatc agatcaaggg tttcagcagg cagccagaca
     1561 tcctgaccat aaacctgtgt ggatttttca ccggtgtaaa tttccatcca ggagatttta
     1621 cgctcgcctt tataggcttt ctcgactgca gcgtcgacca ctttcagcat ggctggggtt
     1681 acatctacac cgattccatc accttcaatg taagggataa tcggattttc aggaacgttg
     1741 agtttgccgt tttgcagggt gatcttcttg ccttgtgccg gaacaactac tttactttcc
     1801 attcacctct ccttcgagcg ctactggttt gctcgtctgc caccacgttg catatgcgtt
     1861 tgcgtcctgc gatacggatg ctttagagca attttttgtt aatgatttgt aattggcttg
     1921 tcaatactac cctattgttt gccgtcatga aagatgcgcg ttattaggct ataatgcggc
     1981 aattcataat ctctgaaaat accatgcaaa aaacttcttt tagaaatcac caggttaagc
     2041 gattcagctc gcaacgttct accaagcgta aacatgaaaa ccagcccacg cgtgtgatcc
     2101 tcttcaataa accctacgat gttcttccgc agttcaccga tgaagccgga cgcaaaacat
     2161 taaaagaatt catcccggtt cagggcgttt atgcagcagg tcgccttgac cgcgatagcg
     2221 aagggttgct ggtgctgacc aataacggcg cgttgcaggc gcgtttaacc cagccgggta
     2281 aacgcaccgg aaaaatctat tatgtgcagg tggaaggtat tcccacacaa gacgcacttg
     2341 aagccttgcg caatggcgta accttaaatg atggccctac cctgcccgca ggcacggagc
     2401 tggttgacga acccgcgtgg ttatggccgc ggaatccacc aattcgtgaa cgcaaaagta
     2461 ttcccaccag ctggctgaag atcaccttat atgaaggacg taatcgccag gtgcgccgca
     2521 tgaccgccca tgttggtttc cccacgctgc gactgattcg ctatgcgatg ggagattact
     2581 ctttggataa tcttgccaat ggcgaatggc gagaagtgac agattaagga tgcaaaatgt
     2641 ttaaaccgca cgttaccgtt gcttgcgtgg tgcacgcaga aggcaaattt ttagtcgttg
     2701 aagagacgat taatggtaaa gcgttatgga accaacctgc cgggcatctg gaagccgatg
     2761 aaaccttagt ggaagccgcc gcccgtgaac tgtgggaaga aaccggcatc agcgcgcagc
     2821 cgcaacactt tattcgtatg catcagtgga ttgcgccaga taaaacgccg tttttgcgtt
     2881 tcctctttgc cattgagctt gagcaaatat gcccgaccca gcctcatgac agcgatatcg
     2941 actgctgccg ttgggtcagc gccgaagaaa ttttacaggc gtcaaatctt cgttcgccgc
     3001 tggtggcgga aagtattcgt tgttatcaaa gcgggcaacg ttatccgctg gagatgattg
     3061 gcgattttaa ctggcctttt acaaagggtg tcatctaaaa ggctgcgtga tagaatacgc
     3121 cgccttgaag ttcaatgtcg tgagtgatcc aatgtctgaa accgcaaaaa aagtaatcgt
     3181 cggcatgtcc ggcggtgtcg attcctccgt ttctgcctgg ctgttgcaac aacagggata
     3241 tcaggtcgaa ggcctgttta tgaagaactg ggaagaagac gacggtgagg aatattgcac
     3301 agcggcagcg gatctggctg atgcccaggc ggtctgcgac aagctcggca ttgaactgca
     3361 caccgttaac tttgctgccg agtactggga caacgtcttc gaactgttcc ttgccgaata
     3421 taaagccggt cgcacgccga atccggatat tctgtgcaac aaggagatca aatttaaagc
     3481 cttcctcgaa tttgccgccg aagatttagg tgccgattat atcgctaccg gtcattacgt
     3541 acgtcgggcc gatgtcgatg gcaagagccg cctgctgcgt ggtctggaca gcaataaaga
     3601 ccagagctac ttcctttata cgctcagcca tgagcagatt gcgcaaagcc tgttcccggt
     3661 cggcgaactg gaaaaaccgc aggtgcgtaa gattgctgaa gatcttggtc tggtcaccgc
     3721 gaagaaaaaa gactctaccg gcatttgctt cattggcgaa cgtaaattcc gcgagttctt
     3781 gggccgttat ctcccggcgc aaccgggcaa aatcattacc gtcgatggcg atgaaattgg
     3841 tgagcaccag gggctgatgt atcacactct cggtcagcgt aaaggtctgg gtatcggtgg
     3901 caccaaagaa ggcaccgaag aaccgtggta tgtggtggac aaagacgtcg aaaacaacat
     3961 tctggttgtc gctcagggcc atgaacaccc gcggctgatg tctgtcgggt tgattgccca
     4021 gcagctgcac tgggtcgatc gcgaaccatt caccggcact atgcgttgca cggtaaaaac
     4081 ccgctatcgc cagaccgaca ttccgtgcac cgtcaaggcg ctggacgacg atcgcattaa
     4141 agtgattttc gatgagccgg ttgccgccgt gacgccgggc cagtctgccg tcttctataa
     4201 cggtgaagtg tgcctcggtg gcggtattat tgagcagcgt ctgccgctgc cggtctgatt
     4261 attatcttta cttaaaacaa ggaagcagtg aacgtggcaa agaattacta tgacatcacc
     4321 ctcgccctgg ccggtatttg tcagtcggca cgcctggtgc aacaactcgc tcaccagggg
     4381 cattgtgatg ccgatgcgct acacgtctca ctcaacagta ttattgatat gaaccccagc
     4441 tcgacgctgg cggtttttgg cggtagcgaa gccaacctgc gcgtcgggct ggaaaccctg
     4501 ctcggcgtgc tcaatgccag cagttgccag ggcttaaacg ccgaattaac ccgctacaca
     4561 ctcagcttga tggtgcttga gcgcaaactc tcctcagcga aaggcgcgct cgacactctg
     4621 ggcaaccgga tcaacggcct gcaacgccag ctcgaacact tcgatttaca gtccgaaacg
     4681 ctgatgagcg cgatggctgc tatctatgtt gatgtgatca gcccgcttgg cccgcgcatt
     4741 caggtcaccg gttcccctgc tgtactgcaa agcccacaag tgcaggcgaa agttcgcgca
     4801 accctgctgg caggcattcg cgccgccgtg ctctggcacc aggtcggcgg cggacgtcta
     4861 caactgatgt tttctcgtaa tcgcctgacc actcaggcaa aacaaattct tgctcattta
     4921 accccggagt tgtgatctat ggaattatcc tcactgaccg ccgtttcccc tgtcgatgga
     4981 cgctacggcg ataaagtcag cgcgctgcgc gggattttca gcgaatatgg tttgctgaaa
     5041 ttccgtgtac aagttgaagt acgttggctg caaaaactgg ccgcgcacgc agcgatcaag
     5101 gaagttcctg cttttgctgc cgacgcaatc ggttaccttg atgcaattgt cgccaatttc
     5161 agcgaagaag atgccgcacg catcaaaaac atcgagcgta ccactaacca cgacgttaaa
     5221 gcggttgagt atttcctgaa agaaaaagtg gcggagatcc cggaactgca cgcggtttct
     5281 gaattcatcc actttgcctg tacttcggaa gatatcaata acctctccca cgcattaatg
     5341 ctgaaaaccg cgcgtgatga agtgatcctg ccgtactggc gtcaactgat tgatggcatt
     5401 aaagatctcg ccgttcagta ccgcgatatc ccgctgctgt ctcgtaccca cggtcagcca
     5461 gccacgccgt caaccatcgg taaagagatg gcaaacgtcg cctaccgtat ggaacgccag
     5521 caccgccagc ttaaccaggt ggagatcctc ggcaaaatca acggcgcggt cggtaactat
     5581 aacgcccaca tcgccgctta cccggaagtt gactggcatc agttcagcga agagttcgtc
     5641 acctcgctgg gtattcaatg gaacccgtac actacccaga ttgaaccgca cgactacatt
     5701 gccgaactgt ttgattgcgt tgcgcgcttc aacaccattc tgatcgactt tgaccgtgac
     5761 gtctggggtt atatcgccct taaccacttc aaacagaaaa ccattgctgg tgagattggt
     5821 tcttccacca tgccgcataa agttaacccg atcgacttcg aaaactccga agggaatctg
     5881 ggcctttcca acgcggtatt gcagcatctg gcaagcaaac tgccggtttc ccgctggcag
     5941 cgtgacctga ccgactccac cgtgctgcgt aacctcggcg tgggtatcgg ttatgcgctg
     6001 attgcgtatc aatccaccct gaaaggcgtg agcaaactgg aagtgaaccg tgaccatctg
     6061 ctggatgagc tggatcacaa ctgggaagtg ctggcagaac caatccagac agttatgcgt
     6121 cgctatggca tcgaaaaacc gtacgagaag ctgaaagagc ttactcgcgg taagcgcgtt
     6181 gacgccgaag gcatgaagca gtttatcgat ggtctggcgt tgccagaaga agagaaagcc
     6241 cgcctgaaag cgatgacgcc ggctaactat attggtcgag ctatcacgat ggttgatgag
     6301 ctgaaataag cctcaaatca gtgccggatg gcgatgctgt ccggcctgct tattaagatt
     6361 atccactttt tcttttttca ctttacctcc cctccccgct ggtttattta atgtttaccc
     6421 ccataaacac ataatcgcgt tacactattt taataattaa gacagggaga aataaaaatg
     6481 cgcgtactgg ttgttgaaga caatgcgttg ttacgtcacc accttaaagt tcagattcag
     6541 gatgctggtc atcaggtcga tgacgcagaa gatgccaaag aagccgatta ttatctcaat
     6601 gaacatttac cggatattgc gattgtcgat ctcggattgc ctgacgagga cggtctgtca
     6661 ctgattcgcc gctggcgtag caacgatgtt tcactgccga ttctggtatt aaccgcccgt
     6721 gaaagctggc aggacaaagt cgaagtatta agtgccggtg ctgatgatta cgtgactaaa
     6781 ccgtttcata ttgaagaggt gatggcgcga atgcaggcat taatgcggcg taatagcggt
     6841 ctggcttcac aggtcatttc gctccccccg tttcaggttg atctctctcg ccgtgaatta
     6901 tctattaatg acgaagtgat caaactgacc gcgttcgaat acaccattat ggaaacgttg
     6961 atacgcaata atggcaaagt ggtcagcaaa gattcgttaa tgctacaact ctatccggat
     7021 gcggagctgc gggaaagcca taccattgat gtactgatgg gacgtctgcg caaaaaaatt
     7081 caggcacaat atccgcaaga agtcattacc accgttcgcg gccagggcta cctgttcgaa
     7141 ttgcgctgat gaaaaaatta ctgcgtcttt ttttcccgct ctcgctgcgg gtacgttttc
     7201 tgttggcaac ggcagcggta gtactggtgc tttcgcttgc ctacggaatg gtcgcgctga
     7261 tcggttatag cgtcagtttc gataaaacta cgtttcggct gttacgtggc gagagcaatc
     7321 tgttctatac ccttgcgaag tgggaaaaca ataagttgca tgtcgagtta cccgaaaata
     7381 tcgacaagca aagccccacc atgacgctaa tttatgatga gaacgggcag cttttatggg
     7441 cgcaacgtga cgtgccctgg ctgatgaaaa tgatccagcc tgactggctg aaatcgaatg
     7501 gttttcatga aattgaagcg gatgttaacg ataccagcct cttgctgagt ggagatcatt
     7561 cgatacagca acagttgcag gaagtgcggg aagatgatga cgacgcggag atgacccact
     7621 cggtggcggt aaacgtctac ccggcaacat cgcggatgcc aaaattaacc attgtggtgg
     7681 tggataccat tccggtggag ctaaaaagtt cctatatggt ctggagctgg tttatctatg
     7741 tgctctcagc caatctgctg ttagtgatcc cgctgctgtg ggtcgccgcc tggtggagtt
     7801 tacgccccat cgaagccctg gcaaaagaag tccgcgaact ggaagaacat aaccgcgaat
     7861 tgctcaatcc agccacaacg cgagaactga ccagtctggt acgaaacctg aaccgattgt
     7921 taaaaagtga acgcgaacgt tacgacaaat accgtacgac gctcaccgac ctgacccata
     7981 gtctgaaaac gccactggcg gtgctgcaaa gtacgctgcg ttctctgcgt agtgaaaaga
     8041 tgagcgtcag tgatgctgag ccggtaatgc tggagcaaat cagccgcatt tcacagcaaa
     8101 ttggctacta cctgcatcgt gccagtatgc gcggcgggac attgctcagc cgcgagctgc
     8161 atccggtcgc cccactgctg gacaatctca cctcagcgct gaacaaagtg tatcaacgca
     8221 aaggggtcaa tatctctctc gatatttcgc cagagatcag ctttgtcggt gagcagaacg
     8281 attttgtcga ggtgatgggc aacgtgctgg ataatgcctg taaatattgc ctcgagtttg
     8341 tcgaaatttc tgcaaggcaa accgacgagc atctctatat tgtggtcgag gatgatggcc
     8401 ccggtattcc attaagcaag cgagaggtca ttttcgaccg tggtcaacgg gttgatactt
     8461 tacgccctgg gcaaggtgta gggctggcgg tagcccgcga aatcaccgag caatatgagg
     8521 gtaaaatcgt cgccgaagag agcatgctgg gcggtgcgcg gatggaggtg atttttggtc
     8581 gccagcattc tgcgccgaaa gatgaataaa tatgtccata cttcacgcat tacgttaagc
     8641 atccgttata atcggttgca gataccagcc tgtggatgct taacatggaa taccaactca
     8701 ctcttaactg gcccgatttt cttgaacgtc actggcagaa acgcccggtg gtgttaaaac
     8761 gcggctttaa taattttatt gacccgatct ctccagacga gttggcgggt ctggcgatgg
     8821 aaagcgaagt tgacagtcga ctggtcagtc accaggatgg caaatggcag gtcagccacg
     8881 gcccgttcga aagctacgat catctcggtg aaaccaactg gtcattactg gtacaggcag
     8941 taaaccactg gcatgagccg accgccgcgc tgatgcgacc gttccgtgaa ctaccggact
     9001 ggcgtattga tgatctgatg atttcttttt ctgtacctgg cggcggcgtc ggcccgcatc
     9061 tcgatcagta cgacgtgttt atcattcagg gtaccggacg tcgtcgctgg cgagtgggcg
     9121 aaaagctgca aatgaaacag cactgcccac atccggatct gttacaggtc gatccgttcg
     9181 aagccatcat cgatgaagag ctggagcctg gtgatattct ttatattccg ccaggattcc
     9241 cgcatgaagg ctacgcgctg gaaaatgcga tgaactattc cgtgggtttt cgcgcgccaa
     9301 atacgcggga attaattagc ggatttgccg attatgtgct gcaacgtgaa ctgggcggca
     9361 actactacag cgatccggat gttccacctc gcgctcatcc tgcggatgtt ctgccgcaag
     9421 agatggataa actgcgtgag atgatgctcg aattgatcaa ccagccggaa cactttaagc
     9481 aatggtttgg cgagtttata tcccagtcac gtcatgaact ggatatcgcg ccgccggaac
     9541 cgccttatca gccagatgaa atctacgatg cgctgaaaca aggtgatgtg ctggtgcgcc
     9601 tgggtggtct gcgcgtattg cgcattggcg acgacgtgta tgccaatggt gagaagatcg
     9661 attccccgca ccgtccggca ctggatgcac tcgccagcaa cattgcgctg actgcggaga
     9721 attttggcga tgcgctggaa gatccgtcat tcctcgcgat gctcgcggcg ctggtcaata
     9781 gcgggtattg gttcttcgaa gggtaagttt ggatttaagc cggatgcagc atcacatgcc
     9841 gcatcccttt tcgcttactt ccgttgcgcc gttaactcgg caatacggac gatcacctgc
     9901 accgcttttt ccataccttc cagagtcaca aactcatgct taccatgata gttgtaaccg
     9961 ccagtgaaca ggttcgggca aggtaatccc ataaacgaca actgcgcgcc gtcggtaccg
    10021 ccgcggatcg gtttcagttc cggttcaata tcgcagtcac gcatcgcctg ctgggcgata
    10081 tcgagaatat gcggatgctc aaccactttc tcgcgcatat tgtagtaact gtcttcaatc
    10141 accaattcaa tgtagcaatc aggatgtaac cctttgccca cttttttggc gatctccatc
    10201 attttacgtt tacgcgcttc aaactgttta cggtcgaaat cacggatgat gtagtgcata
    10261 tcggcccgtt caacggtgcc tttcatgctc gccaggtgat agaaaccttc atagccttct
    10321 gtcatttccg ggctttcatc cgccggaact tccgcatgaa tacgtgccgc cagcgacagc
    10381 gcatttacca tcactccttt cgccgtgccc ggatgaacat tgttaccgac aattttgata
    10441 ttgaccgatg cggcgttgaa gttttcaaac tccagttcgc ctacgccacc accgtcaaca
    10501 gtgtaagccc agcgggcatc gaaggcatca acatcaaaat gtttcgcccc tttgcccact
    10561 tcttcatccg gggtaaaggc gacgcgaata tcaccatgcg gaatgttttt ctgttgcaat
    10621 accgccagcg cggtcatgat ttctgcaata cctgctttgt catcggcacc taacaaggtt
    10681 ttaccatcgg tggtaatcaa cgtctgaccc agtagctgat gcagcaccgg gaacataacc
    10741 ggtgataaag cttcatcgcc gatacccagc gcaatatcgc caccgcgata gttttcaaca
    10801 atttgcggat taacattttt gccgctgcaa tccggtgagg tatccacatg agaaataaag
    10861 ccaatcgccg ggatatcgcc agggacgtta gccggtaacg tcgccatcaa agtgcccttc
    10921 tcacttaagg tcacattgat aagccccatt tcttcgagct gctctttcag cagatgcaat
    10981 aacttccatt ggccttccgt gctgggaacc tgtctcaccc ctgcttttga ttgggtatcc
    11041 agagacacgt agttcaaaaa tcgctcaagt agtttatcca tgtagtcacc ctcacttttt
    11101 gtgacaacat tattaagaag caagaaaaga caaatattgc gtcaggtcac ttttacccct
    11161 gcaagcggga atatttatca gcattacctt aatgaatata aagctaagcc agtaattcac
    11221 aacaaagatt ggcgattcaa gcggtttgcc gtagaattac cgccctcatt aagagtcacc
    11281 aaggtggtta accacaaacc ccgcatcggt aagccatccg ttgcgtttac atgggacaga
    11341 gtaaaaaatt gaataaacaa ccgagttcgc tttcaccgct ggtgcaattg gcgggaattc
    11401 gcaaatgctt tgatggtaaa gaggtcattc cccagctgga tctgactatc aacaatggcg
    11461 agttcctcac gctgcttggc ccttctggct gcggtaaaac aaccgttctt cgcctgattg
    11521 caggtctgga aactgttgat tccggacgca tcatgctgga taacgaggac atcacccacg
    11581 ttccggcgga aaaccgctat gtgaacactg ttttccaaag ctatgcactt ttcccccaca
    11641 tgaccgtgtt cgaaaatgtg gcctttgggt tgcgcatgca aaaaaccccc gctgctgaaa
    11701 ttacgccccg cgtgatggaa gccctgcgga tggtgcagct ggaaaccttc gctcaacgta
    11761 aaccgcatca gctctctggt ggtcaacagc aacgcgtcgc cattgctcgc gcggtggtta
    11821 acaagcctcg tctgttgttg ctggatgagt cgctctcagc gctggattac aaactgcgta
    11881 agcaaatgca gaacgagctg aaagcgttac agcgtaagct tggcattaca ttcgttttcg
    11941 tgactcacga ccaggaagaa gcactcacca tgtcggacag gattgtggtg atgcgcgatg
    12001 gtcgcattga gcaagacggc acgccgcgtg aaatctacga agagccgaaa aacctgtttg
    12061 ttgccgggtt cattggcgaa attaatatgt ttaacgccac ggtgatcgag cgactcgacg
    12121 agcagcgcgt acgcgccaac gttgaaggcc gcgaatgtaa tatctacgtt aacttcgccg
    12181 ttgaaccggg gcaaaaactg catgttctgc tgcgcccgga agacttacgt gttgaagaga
    12241 ttaacgacga caaccacgct gaagggctga ttggttacgt tcgtgagcgt aactacaaag
    12301 gcatgacgct ggagtcggtt gttgaactgg aaaatggcaa gatggtgatg gtcagcgaat
    12361 tcttcaatga agacgatcct gactttgacc actctctcga ccaaaaaatg gccattaatt
    12421 gggtagaaag ctgggaggtc gtactggctg atgaagaaca caagtaagtt ccagaatgta
    12481 gtgattgtca ctattgtcgg ttggcttgtg ttgtttgtct ttctgcccaa cctgatgatc
    12541 attggcacca gctttttgac ccgcgacgac gccagtttcg tcaaaatggt ctttacgctg
    12601 gataactaca cgcgtctgct cgatccgctc tattttgaag tgctattgca ctcgctgaat
    12661 atggcgctga tcgccaccct cgcctgcctg gtgctgggct atccatttgc ctggtttctg
    12721 gcgaagctgc cacacaaggt gcgtccgctg ctgctgtttc tgctgattgt tccgttctgg
    12781 accaactcat tgattcgtat ctacgggctg aaaattttcc tcagcaccaa aggctatctc
    12841 aacgagtttt tgctctggct gggcgttatc gacacaccaa tccgtatcat gttcacgccc
    12901 agtgcggtga ttatcggtct ggtttacatt ctgctgccgt ttatggtgat gccgctgtac
    12961 tccagtatcg aaaaactgga taagccgttg cttgaggcgg cgcgcgatct cggtgccagc
    13021 aagttacaga cttttatccg catcattatt ccactgacga tgccggggat tattgccgga
    13081 tgtctgctgg tgatgctgcc agcgatgggc ctgttctatg tgtccgacct gatgggcggt
    13141 gcgaaaaacc tgctgatcgg taacgtcatc aaggtacagt tcctcaatat tcgtgactgg
    13201 ccgtttggtg cagccaccag cattacgctg actatcgtaa tgggcctgat gttgctggtt
    13261 tactggcgcg cttctcgttt gctgaataag aaggtggaac tcgaatgatc ggtcgactgc
    13321 ttcgcggcgg ttttatgacc gctatctacg cgtacctgta tatcccaatc attattttga
    13381 ttgtgaactc ctttaacagc tcgcgctttg gcatcaactg gcagggtttt accaccaaat
    13441 ggtatagcct gctgatgaac aacgacagcc tgttacaggc agcgcagcat tcactaacaa
    13501 tggcggtgtt ttcggcgacg tttgctacgc ttatcggttc actgacggca gttgcgctgt
    13561 accgttatcg ctttcgcggt aagccgttcg ttagcggaat gctgtttgtg gtaatgatgt
    13621 caccagatat cgtgatggca atttcgctgc tggtgctgtt tatgctgctg ggtattcagc
    13681 ttggcttctg gtcgctgctg ttctcgcata tcaccttctg cctgccattt gtggtggtga
    13741 cggtgtattc gcgcctgaaa ggttttgacg tgcggatgct ggaagcggcg aaagatctcg
    13801 gtgccagcga atttaccatt ctgcgaaaaa tcattctgcc actggcaatg ccagcggtgg
    13861 cggcgggctg ggtgttaagc tttaccctgt cgatggacga tgtggtagtt tcttcgttcg
    13921 tcaccggacc aagttatgaa attctgccgt taaaaattta ttcgatggtc aaagtcggcg
    13981 tatcgccgga agttaacgcg ctggcaacca tattactggt gctgtcgctg gtgatggtta
    14041 ttgccagcca gcttattgct cgtgataaaa cgaaaggtaa cacaggggac gttaaatgaa
    14101 aaaatggtca cgccacctgc tcgcggcggg tgctctggca ctgggcatga gcgccgctca
    14161 cgccgatgac aacaacacgc tgtatttcta caactggacc gagtacgtgc cgccaggact
    14221 gcttgaacag ttcaccaaag aaaccggtat taaggttatc tattcgactt acgagtcgaa
    14281 cgaaaccatg tacgccaagc tgaaaacgta caaagacggt gcctatgacc tagtggttcc
    14341 ttcaacctat tacgtcgata aaatgcgtaa agaggggatg atccagaaga tcgacaagtc
    14401 gaagttaacg aacttcagca atctcgatcc agacatgctc aacaagcctt ttgacccgaa
    14461 taacgactac tccattccgt atatctgggg tgcgacggcg attggtgtta acggtgatgc
    14521 ggtggatccg aaatctgtca ccagctgggc cgatctgtgg aagccagagt acaaaggcag
    14581 cctgctgttg accgacgatg cccgtgaagt gttccagatg gcgctgcgta agctgggcta
    14641 ctccggtaac accaccgatc cgaaagagat tgaagctgca tataacgagc tgaaaaaact
    14701 gatgccaaac gtcgcagcgt ttaactccga taacccggct aacccgtaca tggaaggcga
    14761 agttaacctc ggcatgatct ggaacggttc tgctttcgtt gcacgccagg cgggtacgcc
    14821 tattgacgtg gtgtggccga aagaaggcgg catattctgg atggacagcc tggcgatccc
    14881 ggcaaatgcc aaaaacaaag aaggcgcgct gaaattgatc aacttcctgc tgcgcccgga
    14941 tgtggcaaaa caggttgctg aaactatcgg ttatccaacg ccaaaccttg cggcgcgtaa
    15001 gctgttaagt ccagaagtgg caaacgataa gacgctctac ccggatgctg aaaccattaa
    15061 aaatggcgaa tggcagaatg acgttggcgc agccagcagc atttatgaag agtattatca
    15121 gaagctgaaa gcaggacgtt aattcagcaa gcgggcggtg gtattaccgc ccgtttttta
    15181 gttagcaagg atgtggctaa tgagtcagga cagtaaagtt ttatcccgct ctttcgtcgg
    15241 tattgtggtc cgcttgtagg cctgataagc gtagcgcatc aggcaatgct tcctgcttta
    15301 taagcccttc agcaactttt caacaaattc tggcaccacc tggcttgccg ggccgtaata
    15361 tttctcggca aattcattac caacctgact cggttcaaga ttcagttcaa cggtgtgagc
    15421 accatgtagt ttggcttcgt gtacaaaccc agccgccgga tagacatgcc cggaagtacc
    15481 aatggcaata aagacatccg ccatcgacaa cgccatataa atttcatcca tgccgagtgg
    15541 catttcgcca aaccaaacga cgtgcgggcg caagggtgcc gggaactggc aacaatggca
    15601 tttatcttct ggcgtaacgt ctccggtcca gtcgagaacc tgaccacttt gcgaacaacg
    15661 aactttcagc aattccccat gcatatgaat gacattggta ttacctgcgc gttcatgcag
    15721 gttgtctata ttctgcgtta ccagcaaaaa gcgatcgccg agagcgtctt gcagtttagc
    15781 cagcgcaaga tgcgcggcgt taggctgaat ttctggctgc tgcagctgtc gacgacgggc
    15841 gttataaaac gcttgcacca gttcaggatc gcgatcgaaa ccttccggcg ttgccacatc
    15901 ttcaacccga tgttcttccc acaggccatc tgcggcgcga aaggtacgaa tacctgattc
    15961 cgcagaaatt cctgcccctg tcagtacgag tactcttggt ttttccattg cttccggcac
    16021 cactttatct ctgaaaaaaa tacgctgacg caaacgctcg cgcaggcggc gtttattttt
    16081 acgaaaacga cttaaccgat gaccccgacg cgacagcata gcaacctctg ttgtttaatc
    16141 ggttagatgt aggaaggccg caccacgcat tcctcccgca tcaccgtggc gcgcgcgttc
    16201 aatgcgcgga acacgagcta caggtaagag atgacaaggc aacctgtccg ccagttgcgt
    16261 tgtgattgcc gggaaattcg ataagccacc accaatgacg accaggtcag ggtcaacaat
    16321 ggtcaggata tttcccagag aaaccgctaa taaatccaga taacgctcaa cgtgcgccct
    16381 tgcctgctca tcgccttgat cataaagcgc aataatttcg ggagcctgca acggttgatg
    16441 ataatagtgt tgatacagcc acgcaaaacc gcgaccagac agataatttt caatgcagcc
    16501 atgctgacca cagccgcagc ggcgtaacgg gaaatccaac cccatcatgg ttaacgcatc
    16561 aaccggcaga cgcatatggc caaactcgcc ggtaatgtag cttttgccag taatcggttt
    16621 gccgttgaaa atcagcccgc cgccaacgcc ggtgccgaga attaacccca tcaccagtgg
    16681 atattgcgta aattcatcat cccaggcttc tgaaagggca aaacagttgg catcgttatc
    16741 aaggcgtaca tcgcgatcaa gacgtgcgct caggtcggca cgcagcggtt tgccgctggc
    16801 agcggggaca ttagcggcat acagcttacc atcttctgtt tccggcattc ccggaatacc
    16861 gatgccgaca gaacctttac agccaaaacg ttggtcagct tcagctacca gctcacacac
    16921 tgcatctaaa aatgcgtcat agctgtcacg cggtgtcggc acccgctttt cccactgcaa
    16981 ctgccgaccg ctatcaaaca cgccaagcgc aatttttgtt ccaccaatat caaacccgta
    17041 atacattgcc gctccttgtt ttaatgtact gcctttactg gccgctaagg actcgcgcag
    17101 ggtcaatatt actggcgcgc cgcgccggat accaacttgc caaaagactc agcaacaatg
    17161 ctgtgaccag tacgtagaag acgtccagcc agtgcaattc cgatggcagg aagtcaataa
    17221 aatagatatc gctggagagg aactgatgac cgatcagctt ttcaatccac tcaataatcg
    17281 gggtaagttg cagtgaaaca actacgccga taaccacccc acacaggctg ccgaacagcc
    17341 ctgccagcaa tccataccag acaaagatgg cgcgaattaa accatctttc gcccccagcg
    17401 ttcttaatac tgcgatatcg ccactcttgt ctttcaccgc catcactaag gtggagacga
    17461 tgttgaaaca ggccacgcca atcaccagta ccatcgccag atacataatg gcgcggatca
    17521 tctggatatc gcgatacata tagccgtaag taccaatcca acttttaata taaacatagc
    17581 tgttggtcac ttcacccgca tcgcgtacca gcttattggc gttgaaaaca tccgtcattt
    17641 taagggcaat acctgacacg ctggaaccca tatcaagata ttgttgggca tccgccagcg
    17701 ggatcatggc aaaactgtga tcgagttgac cactcaactg caaaataccg gcaatgtgca
    17761 aacgcacacg ttttggctgc atcagtttat gctcgggatt cgagttgggg atcatgatcg
    17821 acacccaatc gccctgcttc actttcagcg catccgccac gcctttgccg atgataattt
    17881 gctgttcgcc cgctttaaaa ttgcgccacg catccccctg aacaaacgag ggtaatgcgc
    17941 tcagacgctg ttcctgttgt gggttaacgc ccttcacctg gattgcgcgc agattcgctc
    18001 cactttccac cagaccggtg aaattgatat acggcgcagc ggcggcaata cccggcactt
    18061 tctgcacgtt atccagtgct tcctgccagt tagtccatgg ctgatttacc gcttcgattt
    18121 cgccatgcgg caccaccgcc agaatgcggt tattcagttc gcgttcaaag ccgttcatcg
    18181 cgcttaagcc gacgatcaat accgccacgc cgagggcaat gccaatggta gaaatgacag
    18241 agattagcga caccatgccg ccgcgtcgcc gtccgcgact aaaacgcagg ccaatcaata
    18301 acgataaagg catcgccatt actccgcccc catcaggctc agttccgccg tcagacgtcc
    18361 atcacgcatt tccagttggc ggctcatacg tttcgccagt tgcaggtcgt gagtgaccac
    18421 caggaaagcg gtgccctgca agcgattcaa ctccccaagc aactggaaga tgctgtcggc
    18481 gttacgcgca tcgaggttac cggtaggttc atccgccagt accagacgcg gattattgac
    18541 cagcgcacgg gcaatcgcta cacgctggcg ttcgccgcca gaaagttcag atgggcggtg
    18601 attcgcacga tgctccagcc ccaccgcttt taacatctcc agtgcacggc tgttgatttc
    18661 agcgggcttt ttcttgccaa tcagcagtgg catagccacg ttttccaggg cagtaaaatc
    18721 cggcagcagg tggtgaaact gataaataaa gcccagcttc tggttgcgca gttctgcttt
    18781 cgccgctgaa gacagtttgc tcatcggctg accgttaaag atcacatcgc cggaggttgg
    18841 cgtatccagc ccgcctaaca ggtgcagcaa ggtactttta ccggaaccag agctaccgac
    18901 aattgccatc atttcacctt cgcccacgct gaaactgaca ttgtgcagca catcggtttg
    18961 cacactgcct tcctgatagc gtttgcacag gttgtcgcat tgcaacagga tcttattcat
    19021 aacgtaaagc ctcagcgggt tgtgtggcgg cagcgcgcca tgaagggtaa agcgtagaca
    19081 gcagcgcgat agccatcgcc accagcgcaa taacaatgac ctgtaaaggt tcgatagcca
    19141 ccggcagcgc cgcgccatca agcaggacgc cgattatcgg catcagatta tttaactggc
    19201 tggcaagcag tgcgccaagc gccgctccga ggattgcacc gataatcccg gcgctggccc
    19261 cttgcaccat aaagaccatc atgatttgtc gcggagttaa gccttgcgtt tgcaggatcg
    19321 cgacttcgcc ctgcttctcc atcaccatca gccccagcga ggtaataatg ttaaacgccg
    19381 caacagcgac aatcaggctc agcagtaagc ccatcatatt tttttccatg cgtacggcct
    19441 ggaacagctc gcctttacgg tcacgccagt cctgccattt gctgccttca ggcagttttt
    19501 gctgacttaa agagtcaact ttcagcggct catccagcca caaacgccag ccggtaatat
    19561 tggctgccgg ataacgcatc agacgcgagg catcctcaat attcaccagc atttcatagc
    19621 catcgacttc actgttggcg gcgaaagtgc caatcacatt gaacaggcgc tgacttggaa
    19681 tacgccccat cggcgtgaac tggctggcag atggcaccat cacgcggatt tgatcaccgc
    19741 gattgacgcc caactgtgag gcaagttgtt cgccgaggat gacattatat ttccccggct
    19801 cgaggtcagt ttgtttcaca ttgaccagat acggcgttag tggatctttt tgcaccgggt
    19861 cgataccgag catcaccccg accgccacgc tgcgcgcgct ttgcagtacc acatcaccgg
    19921 tagtaatagg tgcgacgcga ttaacgccgt ccagtttgac tgccgtttcc gggagttgct
    19981 gcgggttaag agagccatgc tcagaagaga gaattgcctg tggcatcagg ccaaggatgt
    20041 tgttttgcag ctcgcgctca aagccgttca tcactgacaa tactgtgacc agcgccatca
    20101 ccccgagggt aatgccgatg gtagaaagcc aggagacgaa acgaccgaag cgatccgctg
    20161 cacgcccacg catgtaacgc aggccaataa atagagcgac aggttggtac atgaaatccg
    20221 tctggttgct gtagcaaaga cacgagtata taagcgaaag caagatgggt aaacgtctga
    20281 actgtaagtg atcgtcaggg ctaatctgaa aagcccccgt aaatcaatcg gctaaattgc
    20341 catgttttat catcaatctt gccgcctgat cttaatctga aaaatcatcc aatacagaca
    20401 gatacccatt acacctcggg tcataaccag caggatcaac cgcatgtaaa ttaaaaaaga
    20461 gcgtgttgtc ctgatgaaac aaaaagagct atggattaac cagatcaaag ggttatgtat
    20521 ttgtctggtg gtgatttatc actcggtcat taccttttat ccgcatatga ccacttttca
    20581 gcatccgtta tcggaagtcc tgagcaaatg ctggatctat ttcaatcttt accttgcccc
    20641 ctttcgtatg ccggtttttt tctttatatc cggctatttg attcgccgct atatcgacag
    20701 cgtaccgtgg ggaaattgtc tcgataaacg gatctggaac atcttctggg tgctggcgct
    20761 ttggggcgtg gtgcagtggc tggcgttaag tgcgctgaac cagtggctgg cacctgagcg
    20821 cgatttaagc aatgcctcca atgccgctta tgccgattcc accggtgagt tcctgcacgg
    20881 gatgatcacc gccagcacca gcttatggta tctgtatgcg ttaattgtct atttcgtggt
    20941 atgtaaaatt tttagccgtc tggcgctgcc actattcgcc ttgtttgtac tgctgagtgt
    21001 ggcggttaat ttcgttccca cgccgtggtg gggaatgaac agtgtgatac gcaatttgcc
    21061 ttattacagc cttggcgcat ggtttggcgc aacaataatg acctgtgtta aagaggtgcc
    21121 gttgcgccgc catctgctga tggcttcttt gcttaccgtt ctggcggtcg gtgcctggtt
    21181 gtttactatc tcgctgctgt tgtcgctggt atcgattgtg gtgatcatga agctgtttta
    21241 tcagtacgag caacgattcg gtatgcgctc caccagcctg ctgaatgtga ttggctccaa
    21301 caccattgct atctacacca cccatcgcat tctggttgaa atattcagct taactctgct
    21361 tgcgcaaatg aacgcagcac gctggtcgcc gcaagtcgaa ctgacactcc tgctggttta
    21421 tccctttgtc agtttgttca tctgtactgt tgcgggcttg ctggtaagaa aactttcaca
    21481 gcgtgcattc agcgatctgt tgttctcccc gccttcactg cccgctgccg tcagttactc
    21541 tcgttaaacc atcgccccag ccggggcgat tcaatttgct aaaccatgtc gattacggat
    21601 aatgaacagc attgcttatc agagatatgc ccccatatgt tgaggcatat cctaacgaga
    21661 atctgacaac cgttatgcct gaacaatatc gttatacgct gcccgtcaaa gcgggtgagc
    21721 agcgtctgct gggcgagtta accggcgcag cctgtgcaac gctggtagcg gaaattgccg
    21781 aacgtcacgc cggtccggtg gtactcattg caccagatat gcaaaatgct ctgcgtttgc
    21841 atgatgaaat cagccagttc accgatcaga tggtgatgaa tctggcggac tgggaaactc
    21901 ttccctacga cagtttttcg cctcatcagg acattatctc ctcgcgcctt tccacccttt
    21961 accagctacc gacgatgcag cgtggcgtac tgattgttcc ggtgaatacg cttatgcagc
    22021 gcgtttgccc acacagtttt ctccacggtc atgcgctggt gatgaaaaaa ggtcagcgcc
    22081 tgtcacgaga tgcattacga acccaactgg acagcgccgg ttatcgccat gttgaccagg
    22141 tgatggagca cggcgaatac gccacgcgcg gcgcgttgct ggatctcttc ccgatgggga
    22201 gtgagctgcc ttatcgtctt gatttctttg atgatgaaat cgacagcctg cgggtgtttg
    22261 acgtcgacag ccagcgcacg ctggaggaag tagaagcgat caatctgctg cccgcgcacg
    22321 aatttccgac cgataaagcg gcaattgaac tgttccgcag ccagtggcgc gataccttcg
    22381 aagtgaagcg cgatccggaa catatttacc agcaagtgag taaaggcaca ttacctgccg
    22441 ggatcgagta ctggcagcca ttgttcttca gcgaaccact gccgccgctg ttcagttatt
    22501 tccctgccaa taccttgctc gtgaatactg gcgatctgga aaccagtgcc gaacgtttcc
    22561 aggctgacac gctggcgcgt tttgagaatc gcggcgtcga tccgatgcgc ccgctgttgc
    22621 caccacaatc gctctggctg cgggtggacg agctcttctc agagctgaaa aactggcccc
    22681 gagtgcagct aaaaactgaa catttaccga caaaagccgc gaatgccaat ttaggtttcc
    22741 agaaactgcc agacctggcc gttcaggcgc aacaaaaagc gccgctggat gcgctgcgta
    22801 agttcctcga gactttcgac ggtccggtgg tgttctcggt agaaagtgaa ggtcgccgtg
    22861 aagcgctggg tgaactgctc gcacgaatta aaattgctcc gcaacgcatt atgcgtcttg
    22921 atgaagccag cgaccgtggg cgttatctga tgattggcgc tgccgaacat ggttttgtcg
    22981 ataaggtgcg taatctggcg ctgatctgcg aaagcgatct gctcggtgaa cgtgttgccc
    23041 gccgtcgtca ggattctcgc cgcaccatca accccgatac actgatccgt aaccttgcgg
    23101 agctgcatat tggtcagccg gtggtccatc tggagcacgg cgtcggtcgc tatgccggaa
    23161 tgaccacgct ggaagctggc ggcattactg gcgagtacct gatgctcacc tatgccaacg
    23221 acgccaaact gtatgttccg gtgtcgtcac tgcatctgat tagccgttac gcaggtggcg
    23281 cggaagaaaa cgccccgctg cataaacttg gcggcgatgc gtggtcacgc gcgcggcaga
    23341 aagcggcgga aaaagtgcgt gatgtggcgg cggaattgct ggatatctac gcgcaacgcg
    23401 ccgccaaaga gggcttcgcg tttaaacacg atcgtgagca gtatcagttg ttctgcgaca
    23461 gcttcccgtt tgaaaccacg ccggatcagg cgcaggccat taatgcggta cttagcgaca
    23521 tgtgtcagcc gctggcaatg gatcgtctgg tgtgcggcga tgttggcttt ggtaaaacag
    23581 aagtggcgat gcgcgccgct ttcctggcag tagataacca caagcaggta gcggtgttgg
    23641 tgcctaccac ccttcttgcg cagcagcatt acgacaactt ccgcgaccgt ttcgccaact
    23701 ggccggtacg tatcgaaatg atctcccgtt tccgcagcgc caaagagcag acgcaaatcc
    23761 ttgcggaagt ggcggaaggg aaaatcgata ttctgatcgg tacgcacaaa ctgctgcaaa
    23821 gtgacgtcaa gtttaaagat ttaggcctgc tgattgtcga tgaagaacac cgcttcgggg
    23881 tgcgtcataa agagcgcatt aaagcgatgc gcgcgaacgt ggatattctg acgcttactg
    23941 caacaccgat cccacgtacg ctgaatatgg caatgagcgg aatgcgtgac ctgtcgatta
    24001 tcgccacgcc gcccgcccgt cgtctggcag ttaaaacctt tgtccgtgag tatgacagcc
    24061 tggtggtccg ggaggcgatc ctgcgtgaaa ttttgcgcgg aggacaggtt tattatctct
    24121 acaatgatgt ggaaaacatt cagaaagccg ccgaacggct ggcagaactg gtgcctgaag
    24181 cacggattgc catcggtcac gggcagatgc gcgagcgcga actggaacgg gtgatgaatg
    24241 atttccatca tcaacgtttc aacgtgctgg tttgtaccac cattatcgaa accgggatcg
    24301 acatcccgac agccaacacc attatcattg aacgtgcgga tcacttcggt ctggcgcagc
    24361 tgcaccagtt acgcggtcgc gtcggacgtt cacatcatca ggcatatgca tggctgctga
    24421 cgccgcatcc aaaagcgatg actaccgatg cacaaaaacg tcttgaagcg attgcctcgc
    24481 tggaagatct cggggcaggt tttgcgctgg caacgcacga tctggagatt cgcggcgcgg
    24541 gtgaactgct tggcgaagaa caaagcggct caatggaaac catcggtttc tcgctgtata
    24601 tggagttgct ggaaaacgcc gtcgatgcac tgaaagccgg acgcgagccg tcgctggaag
    24661 atctcaccag ccagcaaaca gaagtcgagc tgcggatgcc gtcgctattg ccagatgatt
    24721 tcatccctga cgtgaacacg cgtctgtcgt tctacaaacg tattgccagc gccaaaacgg
    24781 aaaacgaact ggaagagatc aaagtcgagc ttatcgatcg cttcggcctg ctgccagatc
    24841 cggcgcgtac cctgctggat attgcccgtc tgcgccagca agcgcagaaa ctggggatca
    24901 ggaagctgga aggtaatgag aaaggcgggg tgatcgaatt tgccgagaag aatcacgtta
    24961 atccggcctg gttgattggt ttgctgcaaa aacagccgca gcattaccgc cttgatggtc
    25021 cgacacgcct gaaatttatt caggatttga gtgagcggaa aacgcgtatc gaatgggtac
    25081 gccagtttat gcgtgaactg gaagagaacg cgatcgctta atgtaaatcg ggggcgtttc
    25141 tgcgccccca tctgcaacat ttacaaattc tttgcacttc cctgcactat ccgacactgt
    25201 caccatccat aattcaggct tatctgttta ttacaataac cttatattta ttatggattt
    25261 ttggtgatga tcaaaacgcg tttttctcgc tggctaacgt tttttacgtt cgccgctgcc
    25321 gtggcgctgg cgctaccggc aaaagccaac acctggccgc tgccgccagc gagcagtcgt
    25381 ctggttggcg aaaacaaatt tcatgtggtg gaaaatgacg gtggttctct ggaagccatc
    25441 gccaaaaaat ataacgtcgg ctttctcgct ctgttacagg ctaaccccgg cgttgatcct
    25501 tacgtaccgc gcgcgggcag cgtgttaacg atcccgttgc aaaccctact tccagacgcg
    25561 ccgcgcgaag gcattgtgat caacattgcg gagctgcgtc tctattacta cccgccgggt
    25621 aaaaattcgg taaccgtgta tccaataggt attggtcagt taggtggtga cacgctgaca
    25681 ccgacaatgg tgaccacagt ttcagacaaa cgtgcaaacc caacctggac gccaacggca
    25741 aacatccgcg cccgttataa agcacaggga attgagttgc ctgcggtagt gccggctgga
    25801 ccggataacc caataggcca tcatgcgatt cgtctggcgg cctatggcgg cgtttatttg
    25861 cttcacggta cgaacgccga tttcggcatt ggcatgcggg taagttctgg ctgtattcgt
    25921 ctgcgggatg acgatatcaa aacactcttt agccaggtca ccccaggcac caaagtgaat
    25981 atcatcaaca ctccgataaa agtctctgcc gaaccaaacg gtgcgcgtct ggttgaagta
    26041 catcagccgc tgtcagagaa gattgatgac gatccgcagc tgctgccaat tacgctgaat
    26101 agcgcaatgc aatcatttaa agatgcagca caaactgacg ctgaagtgat gcaacatgtg
    26161 atggatgtcc gttccgggat gccggtggat gtccgccgtc atcaagtgag cccacaaact
    26221 ctgtaaatcc tggataaaaa aaatccccgc gacacggcat gtcgcgggga aaaaatcagc
    26281 agtagcaggc attaatgagg gttaatgctt atttataaat tactgctgtt ccatggaggg
    26341 tattcggacc ggttacagaa gtaatacgga aagattttgc gcccatctca tccgcttttt
    26401 gcgccagctg ctcttccagc gatcccagat ttgtccccgc gttagcactg atagtaccga
    26461 ctttttgttg gccttctggc gttgactgaa cttcgacagc cgcaaagttg gcaaatgaca
    26521 tggagcttaa aatcgccgca gcgatgaggg tttttacgtt tttcataata gtggccttat
    26581 gcagatgaat gacgtcgcaa gaaattactt aacgatcgat aactaaatcg atagatgtga
    26641 tctggatcac atacaagata ttttttataa caatcattaa ttaatttaaa aatctttaaa
    26701 tttcagttaa atagaaagca attattttta tgggatctgg actggtggac agaggtgatt
    26761 atttttataa taacctttca ttcacagtca ccaggtacaa cggcatcatg gcaactgact
    26821 caacacaatg tgtaaaaaaa agccgtggcc gcccaaaagt gttcgacagg gatgccgcgc
    26881 ttgataaggc catgaaattg ttctggcaac acg
//
LOCUS       DADLSA010000041        26812 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.041, whole genome shotgun sequence.
ACCESSION   DADLSA010000041 DADLSA010000000
VERSION     DADLSA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.041"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(33..1220)
                     /gene="eutG"
                     /locus_tag="JN691_001761"
     CDS             complement(33..1220)
                     /gene="eutG"
                     /locus_tag="JN691_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HAY7243223.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQLRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSERKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSQIGRALRTKKSDDSD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(1210..2046)
                     /gene="eutJ"
                     /locus_tag="JN691_001762"
     CDS             complement(1210..2046)
                     /gene="eutJ"
                     /locus_tag="JN691_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HAY7243224.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVM
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(2057..3460)
                     /locus_tag="JN691_001763"
     CDS             complement(2057..3460)
                     /locus_tag="JN691_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HAY7243225.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECRSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(3472..3759)
                     /gene="eutN"
                     /locus_tag="JN691_001764"
     CDS             complement(3472..3759)
                     /gene="eutN"
                     /locus_tag="JN691_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HAY7243226.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(3866..4159)
                     /gene="eutM"
                     /locus_tag="JN691_001765"
     CDS             complement(3866..4159)
                     /gene="eutM"
                     /locus_tag="JN691_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HAY7243227.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(4198..5214)
                     /gene="pta"
                     /locus_tag="JN691_001766"
     CDS             complement(4198..5214)
                     /gene="pta"
                     /locus_tag="JN691_001766"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY7243228.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLAHAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGYKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRTLTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEMNRESSLQ
                     TLVE"
     gene            complement(5211..6014)
                     /gene="eutT"
                     /locus_tag="JN691_001767"
     CDS             complement(5211..6014)
                     /gene="eutT"
                     /locus_tag="JN691_001767"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HAY7243229.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQLVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLGHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            complement(6011..6712)
                     /gene="eutQ"
                     /locus_tag="JN691_001768"
     CDS             complement(6011..6712)
                     /gene="eutQ"
                     /locus_tag="JN691_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HAY7243230.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(6687..7166)
                     /gene="eutP"
                     /locus_tag="JN691_001769"
     CDS             complement(6687..7166)
                     /gene="eutP"
                     /locus_tag="JN691_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAY7243231.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(7179..7514)
                     /gene="eutS"
                     /locus_tag="JN691_001770"
     CDS             complement(7179..7514)
                     /gene="eutS"
                     /locus_tag="JN691_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAY7243232.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(7807..10086)
                     /gene="maeB"
                     /locus_tag="JN691_001771"
     CDS             complement(7807..10086)
                     /gene="maeB"
                     /locus_tag="JN691_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAY7243233.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            10375..11325
                     /gene="tal"
                     /locus_tag="JN691_001772"
                     /pseudo
     CDS             10375..11325
                     /gene="tal"
                     /locus_tag="JN691_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
     gene            11345..13348
                     /gene="tkt"
                     /locus_tag="JN691_001773"
     CDS             11345..13348
                     /gene="tkt"
                     /locus_tag="JN691_001773"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY7243234.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     NPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(13451..14494)
                     /locus_tag="JN691_001774"
     CDS             complement(13451..14494)
                     /locus_tag="JN691_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAY7243235.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSSELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(14620..15195)
                     /gene="nudK"
                     /locus_tag="JN691_001775"
     CDS             complement(14620..15195)
                     /gene="nudK"
                     /locus_tag="JN691_001775"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAY7243236.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(15263..17242)
                     /gene="aegA"
                     /locus_tag="JN691_001776"
     CDS             complement(15263..17242)
                     /gene="aegA"
                     /locus_tag="JN691_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HAY7243237.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            17448..19160
                     /gene="narQ"
                     /locus_tag="JN691_001777"
     CDS             17448..19160
                     /gene="narQ"
                     /locus_tag="JN691_001777"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HAY7243238.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGKPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAISCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEGKGNT
                     PAEV"
     gene            19362..22475
                     /gene="acrD"
                     /locus_tag="JN691_001778"
     CDS             19362..22475
                     /gene="acrD"
                     /locus_tag="JN691_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HAY7243239.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGID
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(22574..22633)
                     /gene="ypfM"
                     /locus_tag="JN691_001779"
     CDS             complement(22574..22633)
                     /gene="ypfM"
                     /locus_tag="JN691_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAY7243240.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            23014..23370
                     /locus_tag="JN691_001780"
     CDS             23014..23370
                     /locus_tag="JN691_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAY7243241.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23374..24501
                     /gene="dapE"
                     /locus_tag="JN691_001781"
     CDS             23374..24501
                     /gene="dapE"
                     /locus_tag="JN691_001781"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAY7243242.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            24529..24729
                     /locus_tag="JN691_001782"
     CDS             24529..24729
                     /locus_tag="JN691_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAY7243243.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(24839..25537)
                     /gene="ypfH"
                     /locus_tag="JN691_001783"
     CDS             complement(24839..25537)
                     /gene="ypfH"
                     /locus_tag="JN691_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7243244.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(25611..>26812)
                     /locus_tag="JN691_001784"
     CDS             complement(25611..>26812)
                     /locus_tag="JN691_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY7243245.1"
                     /translation="HQLVLRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQ
                     PIRWAQGCPLEKMVSEALVFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLS
                     GAHYRTSPLDLRRMMDAPGQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRP
                     RGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYL
                     SVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRL
                     RRDAQALAQWNGEMLPVDPLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSE
                     LALPALRGRLQKNASDAQLCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRI
                     TQWQFFH"
BASE COUNT         6183 a         7375 c         6948 g         6306 t
ORIGIN      
        1 ggataagacg cggcaagcgt cgcatccggc atttattgcg ccgctgcgta caggccgaca
       61 atctgctcca ggctggcggt acgcgggtta ctgcgcagac aaatatcttc cagcgcggcc
      121 tgcgcccatg cgccgtaatg cgcagatgtc gcaccaacat cgcccagtcg tttaccaatc
      181 ccaacttcag caatcagctc acttaccgcg ttaatagcgt cactatcgtc ggatttttta
      241 gttcgcagcg cccgaccaat ctgactaaac cgatcacgac aaaccatccg gttaaactcc
      301 atcaccgttg gcagcaacat agcgttcgcg agaccatgtg gaatatgcag cgccgcgccc
      361 ggctgatgcg ccatcgcgtg gcacaaccca agacccgcac tggaaaacgc cattcccgcc
      421 atacatgaag ccagcaacat gctctcgcgc gcggcaaggt cgtgaccgta gcccaccgct
      481 ttcggcagcg atttgccaat catcgcaatg gcaccaatcg ccagactgtc ggtaaacggt
      541 gtagcgttca gggcgctgta tgcttcaatg gcatgggtta acgcatcaat gccggtcatc
      601 gccgtgacat gcgacggcac accttcggtc aatgcggcgt cgaggatcgc cacatccggc
      661 atcagcgagg catgggctaa cacctgcttg cgctcgctca ccgcgtcgat aatcaccgtt
      721 acattggtgg tttcagagcc ggttccggcg gtagttggaa tggcaatcag cggcaagcgc
      781 agttgcagaa cgctggtttc tgacatctct gccagcgtgc tatccgggtt cgtcaccagc
      841 aacgccacgg ctttcgccgc atccagcacc gagccgccgc caaatgcgat caccccgtca
      901 caacctgact cacgcaactg cgccacggct gcacacacgt cggtaatgca cggttcgccc
      961 accggacatg gccagagcgt catggcgata cctttaacgg ccaggctacg cgtcagcccg
     1021 gcggtcatcc ctgcctgatg caaaaagctg tctgccatca cgaacagatg tttcagccca
     1081 cgcgtttgcg cttgctgtcc gcaactgctc accgagcccg gaccgcaaag cgtcaccggt
     1141 ggaacgctaa atgtttttac ccgttgcaga ttcagggtat cgaacgcctg aaagagcgcg
     1201 gtctgcaatt cattttgcat atagcccctc cgctttctct cgcccactac tggcgatcgc
     1261 cagcggggtc ataaacaaac tgtgctgcgg taaatgcacc tgtaacgccg ggaattgttt
     1321 gcgaaacagt tctgccacgc ccggttgcat acaggagccg cccgccagcc ataaatcagt
     1381 aatgccctgc ccttcaatat gccgggcgac aatgtccgcc attttttcgt acaccggttt
     1441 caccgcaggc caaatctctt caccgtgacc gcgcttgtac tgctccgcct cttccagcga
     1501 aatacggcga tttccggcga gagtcagaga gatgtgatgt ccgccggtgg cttcatccgc
     1561 cgagtacatc actttgccct ttttcacgat ggcaatgccg gtagtgccac cgccgatatc
     1621 cactacaccg gcgttatcca gttgcagtag atccgcgact gcggttggct catccagcac
     1681 atggctcact tccagcccgg cagattccag cacgttgatg gaaatacgcg ggtccgtacc
     1741 tggaggaaat gaagtcgccg catggctaaa acgacgaccg aattgctgct cgagcgtgtc
     1801 gagatggcga cgaacaatgg tgaccgcgcc gaagaaatcc cagacgatgc cgtcgcggac
     1861 cacatcggcc cagtcgaggc ataccgccac cggctgaccg tcgcggtcga caaccatcga
     1921 caccacatcg caggtgccca gatccacccc cagccacagc ggggattccg tcgcggcagg
     1981 cgtctggtta cacagcgttg ccgccgtttg cagacgtggg gtgagccatt gttcgtcgtg
     2041 cgccatcggt tactccttaa acaatgcgaa acgcatcgac taatacacag cgacgcagac
     2101 ggacaaacgt acgcgcgctg gttacccctt caccggttgg cgtggtgatg gtcatggtgg
     2161 tccagccttc cccgcccagt cccagcccgg caatgcacgg tccgttctta acgaaaatgc
     2221 tggtatcaat ggcattcgcc atctggttca tgttctcgat attgcgcgag tgcattgccg
     2281 ccgtgtggtg gcaaccgccc tccagtttca ccgccagcgc aatggcatcc gccacgttgg
     2341 cgacgcgcac gacgggcagc accggcatca tcagttcggt cacggcaaac ggatgttctg
     2401 cagtggtttc cacaaacagc aaacgcgttt cttgcggcac gttcaggccg attgccgccg
     2461 cgattttcgc cgcatcgcga ccaacccagt cacggctgac ggtgcctttt ccgcgctcgt
     2521 cgatattttt cagcaacacc ggttgcagct gctgcgcctg ttctgcggtc agtttcaccg
     2581 cgtgctggcc ttccatcaga cgcatcagtt catcggctac gctatcaaca acaatcagta
     2641 ccttttcgtc ggcacaaatg atgttgttat cgaaagacgc gcctttgacg atggactgag
     2701 cagcacgggc gaggtcagcg gtttcatcca ccactaccgg cgggttgcca gcgcctgcgg
     2761 caatcagacg tttattggtg tgtttacgcg ccgcttctac taccgcttcg ccgccggtca
     2821 ccaccagcag gccgataccc ggaaacttga acaagcgttg cgcggtttcg atatccggat
     2881 ttgccacagt aaccagtaag ttttccggcc cacctgcggc aacaatcgcc tggttgagca
     2941 gcgtaatcgc ccgctgggag acttttttcg ccgccggatg cggggcaaaa atgacgctgt
     3001 tgcccgcggc aatcaggctg atggcgttgt taattacggt tgccgccggg ttagtggaag
     3061 gcgtcaccga agccaccacg ccccagggtg cgttttcaat tagggtcagg ccgttgtcgc
     3121 cagtcagcac ttgcggagag cggcactcaa cgcctggtgt gccgcgcgcc tgagcgacgt
     3181 tttttgcaaa tttatcttca acgcgcccca tgccggtttc actgacggca agttccgcta
     3241 aatctctggc gtgtttttcg cctgcttcac gaatggcagc aatggctaac tggcgcattg
     3301 ccacgctttt taacccttgc tgggcgactt tggctgccgc aacggcgtca tccagggacg
     3361 cgaaaacgcc catctcatga acagcggcgg gcggcgtgtc actgctttgc attttcagca
     3421 gtaccgcttt caccacctgt tcaatatcct gttgattcat gatgttctgc cttatttgtg
     3481 gaaaattacc tgaccgccag acaccacctc atcgacgatg ccaatcacgc acagatcgac
     3541 cggtgacgtt tcgcttttat gcgcctggcg ggcggaactg ccactcacca gcaacaccca
     3601 ctccccggtt cccgcgccaa tattgtcgat ggcgacggca cattgcccgt caggattacc
     3661 ttgtggatca atcatttcca ccatcagcaa tttgtcatgc gccagtccgt gatggcgtac
     3721 ggtacaaaca atttgtccag tgacgactgc cagtttcata cccgcctccg tggcgtattt
     3781 caggtaaaag ctccccctac cctccgcaga aggtaaaatg aaaaaggaga gagcgtgacg
     3841 cccgaatcga cgtcacacag ggtgattaca ggttgctgct atcgcctttc aggccgatcg
     3901 ggaacacttc ttccaggtcg ccgtgcggac gcgggataac gtgtacagat accagctcgc
     3961 cgatacgctg tgccgctgcg gcaccggcat cggtcgccgc tttacaggct gcaacatcac
     4021 cacgaaccat ggcagtacac aggccgccgc caatctgctt aacaccaacc agcttgacgc
     4081 gcgcagcttt caccatcgcg tcagaagcct caatcagtgc aaccaggccc cgggtttcga
     4141 tcattcctaa tgcttccatt gtgctttcct ctttatcagg gtccagaacg ggaccgttca
     4201 ttcaaccagt gtttgtaaac tgctttcgcg gttcatttct gtctgacgcg gcacagctgc
     4261 caccagcgcc agctcgataa tttcctgcac gctacaacca cgagagagat cgtgcatcgg
     4321 cgcggcaagt ccttgtatca atggcccgac ggcacgatat ccgccgagtc gttgtgcgat
     4381 tttgtagcca atatttccgg cttccagcga cggaaaaacc atcacattgg ctttgccctg
     4441 tagcgggctg gcaggcgctt tttgcgccgc cacttccggc acgaaggcgg cgtcaaactg
     4501 caactcgcca tcaaccacca gctttggtgc gcgctcacgg acgatttctg tcgcctgctg
     4561 gacgttggca acgcaggggt gacgggcgct accgttactg gaaaacgaca gcatcgccac
     4621 gcgcggctct tctccggtga gggtgcgcca ggtttcggca ctggcaagcg cgatatccgc
     4681 cagttgcgcc gccgtcggct gtggcaccac gctgcaatcg gcaaagccca acgccggacc
     4741 gctgtactgt ggcagcatca ggaaaatgga tgagagcgtt ttatagcctg gctgcaagcc
     4801 gataatgcgt aatccggcac gcagcacatt cgccgtggaa gagaggttgc ccgcgataca
     4861 gacatccgct ttaccggcgc tgaccattgc tgcggcgaac atcagcggat cggtaagttt
     4921 ttccagcgca tccggcggcg ttttttcgcc cgcgtgggcc agccagcgat gagcaaattc
     4981 ttcccgcatt gcgaggttgc catgcggatc aatcacctgt agcccgtcca tcgccacgcc
     5041 gtgactgagt gcaaactgac gaagttcaaa cggattagcg accagaatgg gcgttgccag
     5101 accttgttga tgtaaatatt gcgcagcttt cagcacacgt tgatctaacg catccggaaa
     5161 aaccactctg gcgggcgctc gcaacgccag ttcacgacaa cgttcaataa tcattgcgtt
     5221 tcccccagtc gttgttggat ctgcttcacc gttagcggcg gcttcgttac gctcaggatc
     5281 atcattacgt agaccgtgct cgaaaggcgg ttcagcgcct gcaagatgtc cgggcgtaat
     5341 acttcaaaac tgcgggtgat aaacacctgc gccgctacgg tttcggtttc acgcactttg
     5401 gtgcgtagca gattgagcaa cgccgcatcg cgtccgtggc tggcttccgg caccagatga
     5461 tcgtgaccaa gataacgcaa cggctgatga gaaagccggt gcagatcttc gtcagaaagc
     5521 ccgacaatcg cctggcagcc cagcggttcc cccagcgcat cggcgcgcat aatgttgcca
     5581 agacgcgaac ggatatccgc cagccacggc tgccacggtt ccgccagttc aatttgcagc
     5641 cacactgcca gcgcaatggt gctgtcgaga accgcgcgaa aacccagacg cggatcgctt
     5701 ttggcgacca ttttttccgc cgacaggtga gtcagcgtat ccggcttttt tgccaccagc
     5761 tggcggcaca gttcgcagca cgcctgcgga tgttcatcgc tactagtcag cccatgaacg
     5821 ggctgcggct gctgctgttc atcgtcaaca aacaggcggc cctgctcgtc aataaacttg
     5881 atgcgcagat ggcggctttc cagcaactcc cgggcagagg gcgtcaggcg actgtccgca
     5941 gggagatgga tctctgcgcc ttcgctgagc gtatggttcg ctcttagcca tgcttcggtg
     6001 atgaaatctt tcatagggat tgccagttag ccggccaggc gacatacagg aatttcacgc
     6061 tggatgtcgt accaaattcg atgctggagc ctttcgggat aaacatcacg tcgcctgctt
     6121 tggcgatcat ggtttcgcct tcgtggcgaa catgcagctc cccttccagc accatgtcga
     6181 tttcgtcgta gttcagggtc cacgggaaga atgcgttctc ccactgcata aaaccggcgg
     6241 ccatgctgct gccgtcgtct ccggtcacaa gatcagtcaa gcccacgcaa tgcggctctg
     6301 cgccgtcgaa acgaccaaac ttgacgctgc tgccatcaat cactttgatg ccgcctttgc
     6361 cggtcaccga tttgaagctc ggctgcattg cgccctgctc cagcgactgt ttttccttca
     6421 tcactttttc catcagctgt gcgaccaggc tttcggtaaa ctggccttcc ggcagctggg
     6481 caatgatggt ttcgcggatg cgctggcttt cggttttgtc ggctggcacg ctggcgggca
     6541 cggacgccgt taccgggatc gattcatcac attcggtgat ggtaaaaccc agtagatccg
     6601 ccacttcacg ggcttccggg gtaataatgc tggcgcgcag aaccacggac attgcctgtt
     6661 cgccgcgcgc gtgtgcttca cgaatatcat tcgctgtgat gagttttttc acctgcttcc
     6721 tcctgtttgg tgagagatgc cagataatcc accagttgct gcacgctttg cgggtcgtgg
     6781 ctgtttagct cgaaaatcgg ctcttcgaaa cccgtctcaa gcaataactt tcgcgttgcg
     6841 gcgacgtcag catctggcat gtccgtttta ctgatgacgg cgatttgccg tttgctgacg
     6901 ccaatatcca gcaacccggc aggcaaccga ctttccgggt catttgcgcc atggacatag
     6961 atcagcatgt ctacatcctg tagcgtggta attaaggcgt gataccagcg gggatggcta
     7021 aaatattcac ccggcgtatc aatatcgccc ttatcattaa attccacggc ctgtgttttt
     7081 ctggcgaggg tataattccc ctgcaacgca ttaaataacg ttgtttttcc tgccccgacg
     7141 ctacccacaa acgcaattcg tttcatgaat gcctcgtatt aacttttggt catttcacaa
     7201 agcgtataat ttaataagcg acctaaaccg ctgacggttt gtgataacgc ttcttctact
     7261 gcgcccaccg agccataaat caccaatgca ccgctgaagc ggtcgagaaa accgatatgc
     7321 acatcagcgg ctttcagcgc taaatcaccg gcgatcatcg ccgtttcgcc gggagttagc
     7381 gtcataatgc caattgcgcc cgcatccgga acgccgattt tcttcgcaag ttcctcaccg
     7441 ggatgcgcga tgagatgcgc cagtgtgacc tgtttgccgg gcacaaattc ctgaatgatg
     7501 cgttctttat ccatgagtcg ccgcctgtga aaaactttcg tgtatcgtga caatttttcg
     7561 tcagagtaaa taacaaaatt cggcaagcgc gtttaaaagg tgaggtagat cactaagaat
     7621 ggagagatat ttactgttat gaataaactt tcatatgcga cgcatgaata taaataaaat
     7681 aatatcgaga tattttatat actgattttt ttattattcg caaactcagt aataagcttt
     7741 gctaatatat ttcaggaaaa ccgcttaccc tgaatattca gggtaagcgt gagagttaag
     7801 aatgaattac agcggttggg tttgcgcttc aaccacggcc agcgccacca tattgacgat
     7861 acgacgcacc gatgcgatcg gcgttaacac gtgaaccggt ttcgccacgc ccatcagcac
     7921 cggaccgaca gtcacacctt ccgagctgga aacgcgcagt aagttgtaac taatgcgggc
     7981 agcttccatg ttcggcatca ccagaatatt ggcagaacct ttcaaagggc tgtccggcat
     8041 acggtcgttg cgaatcgctt ccaccagcgc tgcatcgccg tgcatttcac catcaatcat
     8101 cagatctggc gcacgatccc tgaccagttc cagcgcctga cgcattttgc tcgacgacgg
     8161 gcagtcagaa gaaccaaagt tggagtgcga caacagagca acgcgcggct caataccaaa
     8221 acgacggaca gtttctgccg ccatcaaggt gatctccgcc agctcttctg cgtctggttc
     8281 gtcattgacg taggtatcgg caataaaggt gttaccactc ggcagcagca gcgcgttcat
     8341 tgcacctgcg gtgtgaacgc catcgcgata accaaagaca tttttcacca cgctaaaatg
     8401 ttcatgataa tcgcccaccg taccgcagat cattgcatcg gcttccccac gctgaaccat
     8461 gatcgcgccg atcactgtcg ggttactgat cagcgcacgc tgcgcctgtt cctgagtgac
     8521 accgcgacgc ttcatgatct ggaagtattc ggtccagtac tctttaaagc gcggatcgga
     8581 ttcgttattg acgatttcaa aatcaacgcc cgctttgatc tgcaagccca gtttctgaat
     8641 gcgcatttcg atcacgttcg gacgaccgat aaggatcggt ttcgccagtc ccagcgttac
     8701 cagttcctga gtcgcatgca gaacgcgcgc ctcttcccct tccggcagaa ctacgcgctt
     8761 cggcgctttg cgagcctggg agaaaatcgg cttcataaac aggttggttt tgtaaacgaa
     8821 ctcagtcagc ttgtcgatgt agacgtcgaa atcagcaatc ggacgcgtcg ccacgcccga
     8881 ctccatcgcc gcttttgcga ccgcaggagc gatcttaacg atcaagcgcg gatcaaacgg
     8941 ttttggaatg atgtattccg gaccaaagct cagatcctga acgccatacg ctgaagccac
     9001 cacttcactc tgttctgcat gggccagttc tgcaatcgcg cgtaccgccg ccagtttcat
     9061 ctcttcgttg atggcggttg cgccaacgtc cagcgcgcca cggaagatga acgggaagca
     9121 aagaacgttg ttcacctggt tcgggtagtc ggaacggccg gtacagatga tggcatccgg
     9181 acgcacttct ttcgccagcg gcggcagaat ttccggttcc gggttcgcca gcgccaggat
     9241 cattggtgcc cgagccattt tcttcaccat ttcctgggtc agcactttcg ggccggaaca
     9301 gcccaggaaa atatccgcac cttcaatcac atcatcgagg gtacgtttgc cgtcatccac
     9361 caccgcatat gcggctttgg tttccgccat atttggctca cggccctgat agataacgcc
     9421 ttttgaatcg caaaccacga tgttatgttt ttgcagcccc agcgctacca gcaggttcat
     9481 acaggcgatt gctgcggcac ccgcgccgga aaccaccatc cgcacgtcgg agatgttttt
     9541 ctccaccacg cgcaagccgt tgaggatggc ggcagtgctg atgattgccg tgccgtgctg
     9601 atcgtcgtgg aataccggaa tattcatccg ctcgcgcagt ttctgttcaa tatagaaaca
     9661 ttctggcgct ttaatgtctt cgaggttgat gccgccgaag gtcggttcga gcgcggcgac
     9721 gacttcaata aatttgtccg ggtcgagttc atcaacttca atgtcaaata catcaatccc
     9781 agcgaatttc ttaaatagaa cgcccttgcc ttccatcacc ggtttgcctg ccagcgcgcc
     9841 aatgttgcct aaccccagca ccgccgtacc gttagagatc accgcaacca ggttaccgcg
     9901 cgcggtatat ttgtaggctt ttagcgggtc tttttcgatt tcaagacaag gtgcggcaac
     9961 gcctggtgag taggccagcg ccagatcgcg ctgcgttgcc agaggcttgg ttggagaaac
    10021 ctggattttc cctggaactg gaaattcatg gaaatcaagt gcactttgtt ttaactggtc
    10081 atccatttgg ttgttccttt cacgtaacgt tcacaaataa agtgtgtggg caacagcccc
    10141 tgcccacaac gtggcgcaca ttattaccct gccggagtct acagactttg agcaagtcca
    10201 aactctcacc attaatataa tgttttggta ataatcctat aacactgatg ttacctgctt
    10261 aatccagcaa taccatgcct gtctgctatg cttttttgat gcgtttcgcg aaatttctca
    10321 gaagtgtgaa ttaacgcact catctaacac tttacttttc aaggagtatt tcctatgaac
    10381 gagttagacg gcatcaaaca gttcaccact gtcgtggcag acagcggcga tattgagtcc
    10441 attcgccatt atcatcccca ggatgccacc accaatcctt cgctgttact caaggctgcc
    10501 ggattatcac aatatgagca tttaatagac gatgctatcg cctagggtaa aaaaaatggc
    10561 aagacccagg aacaacaggt ggtcgcagcg tgtgacaaac tggcggtcaa tttcggtgct
    10621 gaaattctca aaatcgtacc cggttgcgtg tcaacagaag ttgatgcacg cctctctttt
    10681 gataaagaaa agagtattga gaaggcgcgc catctggtgg acttgtatca gcaacaaggc
    10741 gttgagaaat cacgcatttt gatcaaactg gcttcgacct gggaaggaat tcgcgcggcc
    10801 gaagagctgg aaaaagaagg tattaactgc aacctgacgc tgctgttttc ttttgcccag
    10861 gcgcgggcct gtgcggaagc aggcgttttt ctgatttcgc cgtttgtagg gcgtatttat
    10921 gactggtatc aggcacgcaa gccgatggac ccgtatgtgg tggaagaaga tccgggcgtt
    10981 aaatcggtgc gcaatatcta cgactactat aagcaacacc actatgaaac cattgtgatg
    11041 ggcgcgagct tccgtcgcac cgaacaaatc ctcgccttaa ccggctgcga tcgactgact
    11101 atcgcaccga atttgttgaa ggagctgcag gaaaaagttt cgccagtggt acgtaaatta
    11161 atcccgcctt ctcagacgtt cccacgccca gcacccatga gcgaagcgga gttccgttgg
    11221 gagcacaatc aggatgcgat ggcggtagaa aaactgtctg aaggcattcg tctgttcgct
    11281 gttgatcaac gcaaactgga agatcttctt gccgccaaac tataaaccag ccacggagtg
    11341 ttatatgtcc cgaaaagacc ttgccaatgc gattcgcgca ctcagtatgg atgcggtaca
    11401 aaaagccaat tctggtcatc ccggcgcgcc gatgggcatg gctgatattg ccgaagtgct
    11461 gtggaacgat tttcttaaac ataatcctac cgacccaacc tggtatgatc gcgaccgctt
    11521 tattctttcc aacggtcacg cgtcgatgct gctctacagt ttgctacatc tgaccggtta
    11581 cgacctgccg ctggaagaac tgaagaactt ccgtcagttg cattcgaaaa ccccaggcca
    11641 cccggagatt ggctatacgc caggcgttga aaccaccacc ggcccgcttg gacaaggttt
    11701 ggcgaacgcc gtcgggctgg cgatagcaga gcgtacactg gcggcgcagt ttaaccagcc
    11761 agaccatgag atcgtcgatc acttcaccta tgtgtttatg ggcgacggct gcctgatgga
    11821 aggtatttcc cacgaagtct gttcgctggc aggcacgctg ggactgggca agctgattgg
    11881 tttttacgat cacaacggta tttccatcga cggtgaaaca gaaggctggt ttaccgacga
    11941 tacggcaaaa cgttttgaag cctatcactg gcatgtgatc catgaaatcg acggtcacaa
    12001 tccgcaggcg gtgaaggaag cgatccttga agcgcaaagc gtgaaagata agccgtcgct
    12061 gattatctgc cgtacggtga ttggctttgg ttcgccgaat aaagcaggta aggaagaggc
    12121 gcacggcgca ccactggggg aagaagaagt ggcgctggca cggcaaaaac tgggctggca
    12181 ccatccgcca tttgagatcc ctaaagagat ttatcacgcc tgggatgccc gtgaaaaagg
    12241 cgaaaaagcg cagcagagct ggaatgagaa gtttgccgcc tataaaaagg ctcatccgca
    12301 actggcagaa gagtttaccc gacggatgag cggtggttta ccgaaggact gggagaaaac
    12361 gactcagaaa tatatcaatg agttacaggc aaatccggcg aaaatcgcta cccgtaaggc
    12421 ttcgcaaaat acgcttaacg cttacgggcc gatgctgcct gagttgctcg gcggttcggc
    12481 ggatctggct cccagcaacc tgaccatctg gaaaggttct gtttcgctga aggaagatcc
    12541 ggcgggcaac tacattcact acggggtgcg tgaatttggc atgaccgcta tcgccaacgg
    12601 catcgcgcac cacggcggct ttgtgccgta taccgcgacg ttcctgatgt ttgttgaata
    12661 cgcccgtaac gccgcgcgga tggcggcact gatgaaagcg cggcagatta tggtttatac
    12721 ccacgactca attggcctgg gcgaagatgg tccgacgcac caggctgttg agcaactggc
    12781 cagcctgcgc ttaacgccaa atttcagcac ctggcgaccg tgcgatcagg tggaagcggc
    12841 ggtgggctgg aagctggcgg ttgagcgcca caacggaccg acggcactga tcctctcaag
    12901 gcagaatctg gcccaggtgg aacgtacgcc ggatcaggtt aaagagattg ctcgtggcgg
    12961 ttatgtgctg aaagacagcg gcggtaagcc agatattatt ctgattgcca ccggttcaga
    13021 gatggaaatt accctgcaag cggcagagaa attagcagga gaaggtcgca atgtacgcgt
    13081 agtttccctg ccctcgaccg atattttcga cgcccaagat gaagaatatc gggagtcggt
    13141 gttgccttct aacgttgcgg ctcgcgtggc ggtggaagca ggtattgccg attactggta
    13201 caagtatgtt ggtctgaaag gggcaattgt cgggatgacg ggttacgggg aatctgctcc
    13261 ggcggataag ctgttcccgt tctttggctt taccgccgag aatattgtgg caaaagcgca
    13321 taaggtgctg ggagtgaaag gtgcctgatg gtgatcgccg gatgctgtat gttcccctca
    13381 ccctaaccct ctccccagaa gggcgagggg acggtccgtg cgctttttag ggccgcatct
    13441 gcgattttgt ttaacgggtg atccacaacg tgggccaggc gtctggccca tgccagttat
    13501 cgcaggtggg ttctgcggcg taacgcacca ggcgaaaacg ctgaccgtca aagcgccagc
    13561 gcgcctgaat gccacaatcg cttaatccgc gccctttcgc taaggtcacc agttcactcg
    13621 atttctcatc aaatgttgcg ttcatcagtt ccagttcatt cgtctcctga ccgttgttga
    13681 acggcaaacg caaccgaacc gggcgcgagg ctagcggctt tttacgcgac acaatccatg
    13741 ccaaatcaat ggtgttatag gcccctgcct cacagctaat catcatcagt gctttatcat
    13801 cggtcagcgc agtgacattc acctcacgac gtaacggatc aagcgagcag cgcagaccat
    13861 tcatccgcca gtttccataa tccagcaaat cgttacgttc ttcgagtgag agtggcgtcg
    13921 gcgttgggtt aaccaccgcg acctctttca gcgcaggcgc gggcggtacg ctgagaggcg
    13981 gttcgtcccc tttcttgatc cacgcggttt cactgccaac gcgcttttgc tgagcatcaa
    14041 taaacaacaa cgccgctttt aagccgctca gagaaatggt ctgattgccg tcgcgtaagg
    14101 tgatcgcctt cccttcctga attatctgca aaaacgcggt gatggttgcc gtatcatcgg
    14161 ttactaataa ccatggtgaa atccgccact tgtcaccact taatgccaac ggctcaccat
    14221 ctaatagcag ccgtggcgct atctcccctt cagacgcatc cggcgacttc aacccaccgc
    14281 gctcaatacg taaaacggca tcggtatgcg ccccagcgct gcggctcagg gtcatcacca
    14341 gtccattatg atcgcccgta ttgcgcgcca cacaaaaatt ttgattatta caggtgacct
    14401 gccagtcgga aaacgcccgt tgcgctggtg ccgcccacac caaagacgtc ggcaacaaag
    14461 cgaaaaaaaa gagaaggaaa atgcgatagc gcatggacgg tacgacccca gaagttcaaa
    14521 caaacagcac aagtcgtatc ttcgttgcgc aggtagcgcg gctcaatcgg atttgtcgga
    14581 ttgagccaat aataaacaac gaaatattat tgttattttt cagtccatta aatgtgacat
    14641 ttgcaaatag ttaagcaata acaccgtctt accgtcacgt atctcgccgg ttttgatcat
    14701 ctccagcgcc tggctgaacg gcagctcgag cacttcaata tcttcatctt caacaccgcc
    14761 accggcgtta gcgcgctgat tgtcactgta ttcggcgata aaaaagtgga ttagctcagt
    14821 cacaccgcca ggcgacatat acagttcaaa taatttgcgc acttcgccca cttcatagcc
    14881 cgtctcttca atcgcttctt tacgaatgca cacttccggt tcgtcgttat ccagcagccc
    14941 ggcgcaggtt tcaatcagct gcccgctttc attgccatta acccaggtag cgacacggaa
    15001 ctgacgaatc agaaccacgc tctttttctt cgcgttgtac aggaggatcg tcgcaccatt
    15061 accgcgatcg taaacttcac gtttatggcg gataacttcg ccatctttgc gcgtgagatc
    15121 gtaagtaatg ttgtgcaggg tgaaatagtt atcggagaga attttgtctt taatgagggt
    15181 gatttgttgc gtcataccga ctccacagcg cgaaatgaac aattatatta cgctgtgaag
    15241 tcgggtttgt ctgcgcaggc tatcagtgag atttgactga ttttaccccc agccagtcaa
    15301 taatcccctg tgccgcatga cgtccttctg ccattgcggt aaccaccaga tccgcaccac
    15361 gcacggcgtc gccaccggcg aagattttcg gattggtggt ctggtaacgg tactggcttt
    15421 ccacatccgc gatgatgcgg ccccatttgt ctaccgttac accgtgcgac tccagccacg
    15481 gcatcccgtg tggattgaag ccaaacgcca taatcaccgc atcggctggc atgacaaatt
    15541 cactgccctc aatcggtaca ggtcgccgac gcccctgggc atccggctcg ccaagacgcg
    15601 tgcgcaggaa acgaattccg caaacgtgac cttgctcatt cagctcaagc gccaccggct
    15661 ggacgttaaa ttcgaagttg gccccctctt cgcgggcgtt cttcacctct ttcttcgagc
    15721 ctggcatgtt agcttcatca cgacgataag cgcaggtgac gttactcgcg ccgtggcgca
    15781 gcgcagtacg cacacagtcc atcgcggtgt cgccgccgcc cagtaccacg acgttaagtc
    15841 cggcggtatt gataaacggc tcttccggta gctcttcgag ccccatcacc tgtttagtgt
    15901 tggcaatgag gaacggcagc gcgtcataaa cgcccggcgc atcttcattg ggtaaacccg
    15961 ctttcatgga acggtaagtg cctacgccaa cgaagaccgc gtcgtattgt tccaaaagcg
    16021 aatccaaaga gacatcttta cccacttcac aattgagttc gaagtgaatc cccatcgcgc
    16081 tgaagatttc ccgacggcgt gccagcaggg atttatccag tttgaaagaa ggaatgccga
    16141 aagtgagcaa gccaccgatt tctggatggc gatcgtacac cgtcaccgcc acgccattgc
    16201 gggtcagaac atccgcacag gccagccctg ccggacctgc cccgataatc gccacccgct
    16261 tgtccacttt ggtgacatga cttaagtcag gacgccaacc tttcgccaac gcctgatctg
    16321 aaatatagcg ttcaatgttg ccgatagtta ccgcgccgtg ctcatcgcga atagtacagg
    16381 caccttcaca caaacggtct tgcggacaaa cgcgtccggt aatttccggc agggtgttgg
    16441 tctggtgaga aagctcgacg gcggcgtcga tgtttccggc tttcaccagt tcaatccact
    16501 gcggaatatg gttatgcagc gggcaggtcc attcacaaac gctgtgctcg ccgaacttaa
    16561 ggcagcgcga ggcttcccgt tgtgcctggt cggcgcgaaa tggcagataa atttcatcaa
    16621 aaccggtttt gcgcgcttca atcgccagtt tatccggctc gccacgcgcg ggcgttgcct
    16681 gcatttgttc gactttactc attaccggca tttcttgtgc agcggtactg gcatgccacg
    16741 gttgatgctc ctgacgtgcg gtgcgcaagc ggcgggattt cgccatgccg gagagtgcga
    16801 cgtcagtgac cagttgcagc gcgtccgccg ggcaattctc gacacaggca ggaccgtttt
    16861 cgcgccctgc acaaaggtcg catttatgcg ccgtggcttt tacttttcct gccgcgacgg
    16921 gtgtcaggac gatttgcatc gtaccaaacg gacaggccac cacgcaggat ttacagccaa
    16981 tacacttttg ctgattgacc tgaatgctgt catcaacgtg gctgattgcg ccattagggc
    17041 agctacgggc gcagggcgca tcttcacaat ggtgacaggt cactgcacta cgttgctgct
    17101 gatgtttgat aaccgtaatt cggggatgaa aatggtgttg gctcaggaca tgttgctcat
    17161 cattgtgagc catgacacag gcgatttcac aagcatgaca acccagacat tgctgactgt
    17221 tggccataat aaaacgattc ataacgacct tcttttttgg ttgtaaaaac cttattcttt
    17281 atatgagtgt tgttattacc cgacttacag gggaaccggc aatgttcatg tgcccagaat
    17341 aagtaactat ttcgctataa actgtggcag atcaaataat cccatcactg actaaattgc
    17401 gtttcagcga actggaacat taatgatttt ttgtggagaa gacgcgtgtg attgttaaac
    17461 gacccgtctc ggccagtctg gcccgggcct ttttttacat tgtgctgctg tcgattcttt
    17521 ccacgggtat cgctctgcta actctggcga gcagtttgcg cgacgctgag gctatcaata
    17581 ttgccggatc gctgcgtatg cagagttacc gcctgggcta cgacttgcaa agtggcagtc
    17641 cacaactcaa tgcacatcgc cagttgtttc agcaggcact gcattcaccg gtattaacca
    17701 acctcaacgt ctggtatgtg ccagaagcag taaaaactcg ctatgcgcat ctgaatgcca
    17761 actggctgga gatgaataat cggctcagca agggcgattt gccgtggtat caggccaata
    17821 ttaataatta tgttaatcag atagacctgt tcgtgctggc tttacagcac tatgctgaac
    17881 gcaaaatgct gctggtggtg gcgatttccc tggctggcgg tatcggtatt ttcacgctgg
    17941 tcttttttac tttgcgccgc atacgccatc aggtggttgc cccgctgaat cagctggtta
    18001 ccgccagtca gcgtattgaa cacgggcagt tcgactcgcc gccgctggat accagtctgc
    18061 cgaatgagct tggtctgctt gcaaaaacct ttaaccaaat gtcgagcgag ctgcataaat
    18121 tgtacctttc gctggaggcg tcagtagaag aaaagacccg cgatctccac gaggccaagc
    18181 gtcgtctgga ggtgttgtat cagtgttccc aggcgctgaa caccagccag attgatgtgc
    18241 attgtttccg ccatattttg cagattgttc gcgacaatga agcagctgaa tatctggagc
    18301 tgaacgttgg ggataactgg cggattagcg aagggaagcc aaaccctgag ctgccaatgc
    18361 agattttacc ggtgacaatg caagagacgg tttacggcga actgcactgg caaaatagtc
    18421 acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc gatgctggga cgcggtttgt
    18481 actttaatca ggcgcagaag cattttcagc agttattgtt gatggaagaa cgtgcgacca
    18541 tcgcccgcga attgcacgac tcgctggctc aggtgctttc ttacttacgt atccagttga
    18601 cgttactgaa gcgttcgata ccggaagata acgccactgc acaaagtatc atggccgatt
    18661 tttcccaggc gttgaatgat gcttatcggc agttacgcga gctgttgact actttccgcc
    18721 tgacgctgca gcaggcggat ctcccctccg cgttgaggga aatgctggat acgttacaaa
    18781 atcaaaccag cgctaaactg accctcgact gccgtctgcc aaccctggca ctggatgcgc
    18841 aaatgcaggt gcatttgttg caaattattc gcgaagcggt gctgaatgcg atgaagcacg
    18901 ccaacgccag cgaaatcgcc atcagttgcg tcaccgcgcc ggacggcaat cacacggttt
    18961 atatccgtga taacgggatt ggtatcggtg aaccgaaaga acccgaaggt cattatggac
    19021 tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac gctgactttt tcacaacctt
    19081 ccggcggcgg cacgttagtg agtattagct ttcgctccgc agagggggag gggaagggga
    19141 acacgccggc tgaggtgtaa ataattaccc tcacaccggc cctctccctt taaaggcgag
    19201 agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt cagcaatctc atgataattt
    19261 acattaactc ctttttttct ccacgattgg ctcgtacctt gccgctacag tgaagcaagt
    19321 caagcctaca acaatacgca gaaacacgag gtcctctttt aatggcgaat ttctttattg
    19381 atcgccccat ttttgcctgg gtgctggcaa tcctgttgtg tctgacaggt accctggcga
    19441 ttttttcatt gcccgttgaa caataccccg atctcgcgcc accgaatgtg cgagtgaccg
    19501 ctaactatcc cggcgcatcg gcccagacgc tggaaaacac cgtgacccag gttatcgagc
    19561 aaaatatgac cggcctcgat aatctgatgt atatgtcatc tcagagcagt ggcaccggtc
    19621 aggcatctgt cactttaagt tttaaagcag gcaccgatcc ggacgaagcc gtgcagcaag
    19681 tacaaaacca gctgcaatca gccatgcgaa agttaccgca ggcggtgcaa aatcagggcg
    19741 tgacggtgcg taaaaccggc gataccaaca ttctgaccat tgccttcgtc tctaccgatg
    19801 gctcgatgga taaacaggat attgctgatt atgttgccag taatattcag gacccgttaa
    19861 gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc gcaatattcc atgcgtatct
    19921 ggctggaccc ggcgaaactc aacagtttcc agatgacggc taaagatgtc actgatgcca
    19981 ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg tggtacacct tccgtcgata
    20041 agcaggcgct caacgccacc attaacgccc agtcactgct gcaaacacca gaacagttcc
    20101 gcgatatcac cttgcgggtg aatcaggacg gctcagaggt aaggctgggc gatgtcgcca
    20161 ccgtcgaaat gggggcggag aaatacgatt atcttagccg cttcaatggt aagccagcct
    20221 ccgggttggg ggtaaaactg gcctccggcg caaacgaaat ggcgacagcg gagctggtgc
    20281 tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg actggaatac aaggtggcgt
    20341 atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt ggtgaaaacg ctgctggaag
    20401 ctatcgctct ggttttcctc gttatgtatc tgttcctgca aaacttccgc gccacgctga
    20461 tacccactat cgccgtgccg gtggtgttga tgggaacctt ctccgtactt tacgccttcg
    20521 gttacagcgt caacacctta accatgttcg cgatggtgct ggcgatcggt ctgctggtgg
    20581 atgacgccat cgtggtggtg gaaaacgtcg aacgtattat gagtgaggaa ggactcactc
    20641 ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg ggcactggtc gggattgcga
    20701 tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg cggcaccacc ggtgccatct
    20761 atcgccagtt ctctattacc attgttgcgg cgatggtgct gtcagtactg gtagcgatga
    20821 tcctcactcc ggctctgtgt gccacgctac ttaagccact gaaaaaaggt gagcatcacg
    20881 ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa ccgcaacgcc gaacgctacg
    20941 aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg gattgtgatt tatgtcctgc
    21001 tgcttggcgg catggtgttc ctgttcctgc gtttgccgac gtcgttctta ccgctggaag
    21061 accggggcat gtttactacc tcggtacaat tgcccagcgg ttcaacccaa caacagaccc
    21121 tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca tgaaaaagac aacatcatgt
    21181 cggtgtttgc caccgttggt tctggccctg ggggtaacgg gcaaaacgtg gcgagaatgt
    21241 ttatccgcct gaaagactgg agcgaacgcg acagtaagac cggcacctcg tttgccatta
    21301 tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc tcgcgttatc gccagcagcc
    21361 caccagcaat tagcggtctt ggtagttctg caggttttga tatggagttg caggaccacg
    21421 ctggagcggg tcacgatgcg ctgatggcag cacgtaatca gttgctggcg ctggcggcgg
    21481 aaaacccgga gctaacccgt gtgcgccata acggcctcga tgacagtccg cagttgcaga
    21541 ttgatatcga ccagcgtaaa gctcaggcgc tgggcattga tatcgacgat attaacgaca
    21601 cactgcaaac cgcctggggt tcgagctatg tgaatgactt tatggatcgc ggtcgcgtga
    21661 agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct gccagatgac atcaatctct
    21721 ggtatgtccg aaataaagat ggcggcatgg tgcccttctc tgctttcgcg acctcacgct
    21781 gggaaacagg ctcgccgcgt ctggaacgct ataacggtta ttctgcggtt gagattgttg
    21841 gggaagccgc accgggggtc agtaccggta cggcgatgga tattatggaa tcgttagtga
    21901 agcagctgcc aaacggcttt ggtctggagt ggacagcgat gtcgtatcag gagcggcttt
    21961 ccggcgcgca ggctccggcg ctgtacgcca tttccttgct ggtggtattc ctgtgtctgg
    22021 cggcattgta tgaaagctgg tcggtaccgt tctcggtaat gctggtcgtg ccgctggggg
    22081 taatcggcgc gctgctggca acctggatgc gcgggctgga aaacgacgtt tacttccagg
    22141 tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc catcctgatc gtcgagtttg
    22201 ctaacgagat gaaccaaaaa ggccacgacc tgtttgaagc gacgctccac gcctgccgtc
    22261 agcgtttacg cccgattctg atgacctcgc tggcatttat cttcggcgta ttgccaatgg
    22321 caaccagcac gggtgccggt tccggtggtc agcatgcggt gggtactggc gtaatgggcg
    22381 ggatgatttc agctactatt ctggctattt acttcgtgcc gctgttcttt gtgctggtgc
    22441 gccgccgctt cccgctgaag ccgcgcccgg aataagcaat aaaaaaggcg acatgccaat
    22501 gtgtcgcctt tttcaacttt ccgattaaga acctgctcag cgggttcttg ctgtttatac
    22561 tttgtctcag gaattactta cgaagcataa cttcgataaa gtctttccag ttccccagtt
    22621 cacgttcaat cataacaacc tctcttataa ttatgggtat tctacggaaa caatataccg
    22681 tggtgaagct aatttactcg attgctgcga tgactacctc cgggggacaa accttatgta
    22741 aatactatgg tcctgcggtg ataatttgta tgtgatacac agcaacattt cgagatattc
    22801 atacggcatc taatactgat ttaattctgg ttaaaataca gacagataac aagatgaata
    22861 ttcttaatgt ttacgttaaa aatgtttaat attatttaat agttgttaat ttgaatactt
    22921 cgataatgtt atattttctg ataatcattt gcaggcaaaa tgttttcacc cttaaatgag
    22981 tatttattct cataaatcga aaaaggattc attatggtta cactttacgg catcaaaaat
    23041 tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca ataacatcga ctatcgtttt
    23101 catgattacc gcgtcgatgg gctggacagc gaattattga acggttttat caacgaatta
    23161 ggctgggaag cgttactcaa cacccgtggt acaacctggc gtaaactgga cgaaactacc
    23221 cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa tgactgaaat gcctgcaatt
    23281 atcaaacgtc cattgctctg cgcgcctggt aagcctatgc tgctgggttt cagtgattcc
    23341 agttatcagc aatttttcca tgaggtgtag tctatgtcgt gcccggttat tgagctgaca
    23401 caacagctta ttcgccgccc ttccctgagt cctgatgacg caggatgtca ggctttgttg
    23461 attgaacgtt tgcaggcgat cggttttacc gttgaacgca tggattttgc cgatacgcag
    23521 aatttttggg catggcgtgg gcaaggtgaa acgttagcct ttgccgggca taccgacgtg
    23581 gtgccgcctg gcgacgccga tcgttggatc aatccgccgt ttgaacccac cattcgtgac
    23641 ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct cgctggcggc gatggtggta
    23701 gcagcagaac gttttgtcgc acaacatccc aaccatacgg ggcgactggc atttctgatc
    23761 acttctgatg aagaagccag tgcccacaac ggtacggtaa aagtcgtcga ggcgttaatg
    23821 gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac cgtcgagtat cgaagtggta
    23881 ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct gcaacctcac cattcatggc
    23941 gttcaggggc atgttgccta cccacatctg gctgacaatc cggtacatcg cgcagcacct
    24001 ttccttaatg aattagtggc tattgagtgg gatcagggca atgaattctt cccggcgacc
    24061 agtatgcaga ttgccaatat tcaggcggga acgggcagta acaatgttat tccgggtgaa
    24121 ctgtttgtgc agtttaactt ccgcttcagc accgaactga ctgatgagat gatcaaagcg
    24181 caggtacttg ccctgcttga aaaacatcaa ctgcgctata cggtggattg gtggctttcc
    24241 gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg cggtcgttaa cgcggttgag
    24301 cactataatg aaattaaacc gcagctactg accacaggcg gaacgtccga cgggcgcttt
    24361 attgctcgca tgggggcgca ggtggtggaa ctcgggccgg tcaatgccac tattcataaa
    24421 attaatgaat gtgtgaacgc tgccgacctg cagctacttg cccgtatgta tcaacgtatc
    24481 atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg aaataagcat ggactggctg
    24541 gctaaatatt ggtggattct ggtgattgtc tttttggtag gcgtcctgct gaacgtgatt
    24601 aaagatctca agcgcgtcga ccataagaaa tttctcgcca acaagccgga gcttcccccg
    24661 catcgtgatt tcaacgataa gtgggacgat gacgacgact ggccgaaaaa ggatcaaccg
    24721 aagaagtaag ctgagtgctc aaccgtaggc cggataaggc gttaacgccg catccggcaa
    24781 tggtgaacga tgcctgatgc gacgctcacg cgtcttatca ggccaggtaa tctgtctttt
    24841 acatcatttc aatcacatca tcatcgccag gcttaccgcc acttagcgct tcgtcgaaat
    24901 aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa ttgcatactg cgattgtcga
    24961 tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc accaccggca ctgattaacg
    25021 cttcctgcgc cgcaactgca tgcgccagat cgataaccgg atcttcacca ccatgaatca
    25081 agtgaattgt cgtggctgta gacgcggtct ccggcaggct cgcatagcgt ccgttaaagg
    25141 caatgacgcg cgaagcaagg cccggttcgg ctttaatgct ctccagcgcc ataatcgcgc
    25201 cttgtgagaa accaattagc gccgtggcgt tagcccctac tccgctctgt ttctgccagt
    25261 agcgtaccgt ttcaataaac gtcggcatga tggcatccac acgcgcctgg cgattatctt
    25321 ccgtaatacc ctgcactgaa aaccactgac gccccgccgg attaccgctc ggctccgcgc
    25381 caccgacact caccaccagc gcatcaggaa acagcggtgc aaaccagctg cctatttccc
    25441 ccattgccac cgggttatcc ccgacaccgt ggaaaagcag taacaactgt tgtgcaggtt
    25501 tatccgggct ttgaacaaca aaatggtcat gtttcatggc gaactcctta actgatgtca
    25561 ttaattttac gccgctgact cacaatgacc atgggagaaa attgaaggag tcagtgaaaa
    25621 aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc gaacctcatt aagtgcgaac
    25681 agcgcctgcg cggcctcttc ccgctggcga accagtaaca tcttgcggcc tgaaagttta
    25741 agtgtggtac ataactgcgc gtcactggcg tttttctgta aacgcccacg cagcgccggt
    25801 aatgccagtt cactggtttg tagcagacgc aataagcaac ctaacgatgt taatagcgga
    25861 cgatgagcga aagcaaaacc ggccagttca agccagtcgt cgtcagaaag gatggcatcg
    25921 tttagtggat caacaggaag catttcgcca ttccactgcg cgagagcttg cgcatcgcga
    25981 cgtaaacggt aatgctcacg ttcagccagc tgtttacccg catcactcat cggtaacagc
    26041 gccatcgccg tatagcaacc gctgctggct tcccgatgat tacccatccg caccagcaca
    26101 aaaccgcagc gttgccagaa acgccataac tccccggtgt aaccaaaact caccgaaaga
    26161 tagtcgaggt catgcgtata ttgcaaagct ccagcaataa gttgccgccc tgtgccttcc
    26221 cgctgacgag ccggatgaac tgctatccgg ctaacccgcc ttccgcgcaa tgtcgccgcc
    26281 agtggattgc tgccgtgcgc cgccagcgac tgggccacca gattaccccg cgggcgacga
    26341 aaacctgccc ataccgcctg actgagttct tgagataatc cgccctcatc caccagccac
    26401 aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa aatgttgccc tggtgcatcc
    26461 atcatccggc gtaaatccag cggcgaggtc cggtagtgcg caccagacaa taactgataa
    26521 acctttaacg gcgtttctgg ctcgcttcgc cataacgttt gttcaaatgc ggaaatgacg
    26581 atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa aaaccagtgc ctcactaacc
    26641 attttttcca gcgggcatcc ctgtgcccag cggatcggct gttgcagttc aaaacggtgt
    26701 aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac gtccggtacc ttcgtagccc
    26761 tgcaccgtag tggttaacaa cgttcgagga aaacgcaata ccagttgatg ca
//
LOCUS       DADLSA010000042        26624 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.042, whole genome shotgun sequence.
ACCESSION   DADLSA010000042 DADLSA010000000
VERSION     DADLSA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.042"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..375)
                     /locus_tag="JN691_001785"
     CDS             complement(<1..375)
                     /locus_tag="JN691_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243246.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINKFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQ"
     gene            899..2827
                     /gene="thrS"
                     /locus_tag="JN691_001786"
     CDS             899..2827
                     /gene="thrS"
                     /locus_tag="JN691_001786"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAY7243247.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2831..3373
                     /gene="infC"
                     /locus_tag="JN691_001787"
     CDS             2831..3373
                     /gene="infC"
                     /locus_tag="JN691_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAY7243248.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3470..3667
                     /gene="rpmI"
                     /locus_tag="JN691_001788"
     CDS             3470..3667
                     /gene="rpmI"
                     /locus_tag="JN691_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAY7243249.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3720..4076
                     /gene="rplT"
                     /locus_tag="JN691_001789"
     CDS             3720..4076
                     /gene="rplT"
                     /locus_tag="JN691_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAY7243250.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            4199..4243
                     /locus_tag="JN691_001790"
     CDS             4199..4243
                     /locus_tag="JN691_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAY7243251.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4526..5509
                     /gene="pheS"
                     /locus_tag="JN691_001791"
     CDS             4526..5509
                     /gene="pheS"
                     /locus_tag="JN691_001791"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAY7243252.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5524..7911
                     /gene="pheT"
                     /locus_tag="JN691_001792"
     CDS             5524..7911
                     /gene="pheT"
                     /locus_tag="JN691_001792"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAY7243253.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQTSLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7916..8215
                     /gene="ihfA"
                     /locus_tag="JN691_001793"
     CDS             7916..8215
                     /gene="ihfA"
                     /locus_tag="JN691_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAY7243254.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8316..9296
                     /gene="btuC"
                     /locus_tag="JN691_001794"
     CDS             8316..9296
                     /gene="btuC"
                     /locus_tag="JN691_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAY7243255.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9359..9910
                     /gene="btuE"
                     /locus_tag="JN691_001795"
     CDS             9359..9910
                     /gene="btuE"
                     /locus_tag="JN691_001795"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAY7243256.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9910..10659
                     /gene="btuD"
                     /locus_tag="JN691_001796"
     CDS             9910..10659
                     /gene="btuD"
                     /locus_tag="JN691_001796"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAY7243257.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSVLDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10737..11201
                     /locus_tag="JN691_001797"
     CDS             10737..11201
                     /locus_tag="JN691_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7243258.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11448..12161
                     /gene="ydiV"
                     /locus_tag="JN691_001798"
     CDS             11448..12161
                     /gene="ydiV"
                     /locus_tag="JN691_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HAY7243259.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12225..13661
                     /locus_tag="JN691_001799"
     CDS             12225..13661
                     /locus_tag="JN691_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAY7243260.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13665..13856)
                     /gene="hemP"
                     /locus_tag="JN691_001800"
     CDS             complement(13665..13856)
                     /gene="hemP"
                     /locus_tag="JN691_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAY7243261.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13988..15034)
                     /gene="aroH"
                     /locus_tag="JN691_001801"
     CDS             complement(13988..15034)
                     /gene="aroH"
                     /locus_tag="JN691_001801"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAY7243262.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(15191..16024)
                     /gene="ppsR"
                     /locus_tag="JN691_001802"
     CDS             complement(15191..16024)
                     /gene="ppsR"
                     /locus_tag="JN691_001802"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAY7243263.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16357..18735
                     /gene="ppsA"
                     /locus_tag="JN691_001803"
     CDS             16357..18735
                     /gene="ppsA"
                     /locus_tag="JN691_001803"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAY7243264.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18792..20492)
                     /gene="fadK"
                     /locus_tag="JN691_001804"
                     /pseudo
     CDS             complement(18792..20492)
                     /gene="fadK"
                     /locus_tag="JN691_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707412.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20495..20788)
                     /locus_tag="JN691_001805"
     CDS             complement(20495..20788)
                     /locus_tag="JN691_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY7243265.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20785..22074)
                     /locus_tag="JN691_001806"
     CDS             complement(20785..22074)
                     /locus_tag="JN691_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7243266.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22130..23068)
                     /locus_tag="JN691_001807"
     CDS             complement(22130..23068)
                     /locus_tag="JN691_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAY7243267.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYDPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23088..23852)
                     /locus_tag="JN691_001808"
     CDS             complement(23088..23852)
                     /locus_tag="JN691_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HAY7243268.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24168..25079
                     /locus_tag="JN691_001809"
     CDS             24168..25079
                     /locus_tag="JN691_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7243269.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLINTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(25122..26273)
                     /locus_tag="JN691_001810"
     CDS             complement(25122..26273)
                     /locus_tag="JN691_001810"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY7243270.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYSRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(26287..>26624)
                     /locus_tag="JN691_001811"
     CDS             complement(26287..>26624)
                     /locus_tag="JN691_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243271.1"
                     /translation="GSLKTEITDGKLNIVQEGRVKKFIRELPEITFSGKIALERGLDV
                     RYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPELKLMDERLFIDAAM
                     GFVLPEAAH"
BASE COUNT         6580 a         6438 c         6932 g         6674 t
ORIGIN      
        1 ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg tggtttccag
       61 taataaggaa atgatttctt ttttatagtg taatgtaatg agtatatttt ctgcatggat
      121 gggtgaagtg gcaaagttaa tggtaaaaag agcagtggaa taacaatttt gtgatatttt
      181 tcgacagttt gcggtaaatt cctgagtaac acagtatgac agcatcgaac ttaagtcaga
      241 ggtatcaagt agggtgatga gctggcgcgc aaatttatta ataggctctg atgaaggtgt
      301 tggtatgtcg ctatcgatgt gcaatctatg aggagactta taatgagttc tgataatgat
      361 atcgttttga ctcataatct attcgcctta tatatatctg gaggttttga ggatgatgat
      421 taattattat cgccagaacg aaatgtgcac gaaaaattgt tataagaatt gtgtcacttt
      481 tatcattgtg atatgaccac ttcgtcatta atctcatgag cgtttaaaat actaccgcca
      541 aatcacgcaa cgaactttgc tggattcagt ttatgctgct gtaaatccgc tcgcagtaaa
      601 cctttcagac gcaccgtgat gttatcagtg gttctttaag cgttttgctg gtgtactcac
      661 tacaaacgaa ttgcgaatcg aatcaatgtg aaacggaaag gtacaatctc ccttttttta
      721 gtgttaacat cgttcaaccg ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct
      781 gattgcgaac caatttagca tttgttggct agatggtttc gcaatgaact gttaataaac
      841 aaatttttct ttgtatgtga tctttcgtgt gggtcaccac tgcaaataag gatataaaat
      901 gcctgttata actcttcctg atggcagcca acgccattac gaccacgctg taagccccat
      961 ggatgttgcg ctggacattg gtccaggtct ggcgaaagcc tgtatcgcag ggcgcgttaa
     1021 tggcgaactg gttgatgctt gcgatctgat tgaaaacgac gcacaactgt cgatcattac
     1081 cgccaaagac gaagaaggtc tggagatcat tcgtcactcc tgtgcgcacc tgttagggca
     1141 tgcgattaaa caactttggc cgcataccaa aatggcaatc ggcccggtta ttgacaacgg
     1201 tttttattac gacgttgatc ttgaccgcac gttaacccag gaagatgtcg aagcactcga
     1261 gaagcggatg catgagcttg ctgagaaaaa ctacgacgtc attaagaaga aagtcagctg
     1321 gcacgaagcg cgtgaaactt tcgccaaccg tggggagagc tacaaagtct ccattcttga
     1381 cgaaaacatc gcccatgatg acaagccagg tctgtacttc catgaagaat atgtcgatat
     1441 gtgccgcggt ccgcacgtac cgaacatgcg tttctgccat catttcaaac taatgaaaac
     1501 ggcaggggct tactggcgtg gcgacagcaa caacaaaatg ttgcaacgta tttacggtac
     1561 ggcgtgggca gacaaaaaag cacttaacgc ttacctgcag cgcctggaag aagccgcgaa
     1621 acgcgaccac cgtaaaatcg gtaaacagct cgacctgtac catatgcagg aagaagcgcc
     1681 gggtatggta ttctggcaca acgacggctg gaccatcttc cgtgaactgg aagtgtttgt
     1741 tcgttctaaa ctgaaagagt accagtatca ggaagttaaa ggtccgttca tgatggaccg
     1801 tgtcctgtgg gaaaaaaccg gtcactggga caactacaaa gatgcaatgt tcaccacatc
     1861 ttctgagaac cgtgaatact gcattaagcc gatgaactgc ccgggtcacg tacaaatttt
     1921 caaccagggg ctgaagtctt atcgcgatct gccgctgcgt atggccgagt ttggtagctg
     1981 ccaccgtaac gagccgtcag gttcgctgca tggcctgatg cgcgtgcgtg gatttaccca
     2041 ggatgacgcg catatcttct gtactgaaga acaaattcgc gatgaagtta acggatgtat
     2101 ccgtttagtc tatgatatgt acagcacttt tggcttcgag aagatcgtcg tcaaactctc
     2161 cactcgtcct gaaaaacgta ttggcagcga cgaaatgtgg gatcgtgctg aggcggacct
     2221 ggcggttgcg ctggaagaaa acaacatccc gtttgaatat caactgggtg aaggcgcttt
     2281 ctacggtccg aaaattgaat ttaccctgta tgactgcctc gatcgtgcat ggcagtgcgg
     2341 tacagtacag ctggacttct ccttgccgtc tcgtctgagc gcctcctatg tgggcgaaga
     2401 caacgagcgt aaggtaccgg taatgattca ccgcgcaatt ctggggtcga tggagcgttt
     2461 catcggtatc ctgaccgaag aattcgctgg tttcttcccg acctggcttg cgccagttca
     2521 ggttgttatc atgaatatta ccgattcaca gtctgaatac gttaacgaat tgacgcaaaa
     2581 actatcaaat gcgggcattc gtgttaaagc agacttgaga aatgagaaga ttggctttaa
     2641 aatccgcgag cacactttgc gtcgcgtccc atatatgctg gtctgtggtg ataaagaggt
     2701 ggaatcaggc aaagttgccg ttcgcacccg ccgtggtaaa gacctgggaa gcatggacgt
     2761 aaatgaagtg atcgagaagc tgcaacaaga gattcgcagc cgcagtctta aacaattgga
     2821 ggaataaggt attaaaggcg gaaaacgagt tcaaacggcg cgccctaacc gtatcaatgg
     2881 cgaaattcgc gcccaggaag ttcgcttaac aggtctggaa ggcgagcagc ttggtattgt
     2941 gagtctgaga gaagctctgg agaaagcaga agaagccgga gtagacttag tcgagatcag
     3001 ccctaacgcc gagccgccgg tttgtcgtat aatggattac ggcaaattcc tctatgaaaa
     3061 gagcaagtct tctaaggaac agaagaaaaa gcaaaaagtt atccaggtta aggaaattaa
     3121 attccgtcct ggtacagatg aaggcgacta tcaggtaaaa ctccgcagcc tgattcgctt
     3181 tctcgaagag ggtgataaag ccaaaatcac gctgcgtttc cgcggtcgtg agatggcgca
     3241 ccagcaaatc ggtatggaag tgcttaatcg cgtgaaagac gatttgcaag aactggcagt
     3301 agtcgaatcc ttcccaacga agatcgaagg ccgccagatg atcatggtgc tcgctcctaa
     3361 gaagaaacag taaggccttc aagtagcact gtctgtggag cctttgggct tcacaggcat
     3421 tgttcgcctt tgtttcgttt attaacaatg cgaagtggaa gttattaaaa tgccaaaaat
     3481 taagaccgta cgcggtgctg ctaagcgctt caaaaaaacc ggtaaaggtg gttttaagca
     3541 caagcacgct aacctgcgtc acattctgac taaaaaagcg accaaacgta aacgtcacct
     3601 gcgtccgaaa gccatggttt ccaaaggcga tctgggcctg gtaatcgcgt gcctgccgta
     3661 cgcataagcc gttaacgttt ttaacttttt aattagaata tagatacagg agagcacata
     3721 tggctcgcgt aaaacgtggt gttattgcac gcgcacgtca caagaaaatt ttgaaacaag
     3781 ctaaaggcta ctacggtgcg cgttctcgcg tataccgcgt tgccttccag gctgttatca
     3841 aagctggtca gtatgcttac cgtgaccgtc gtcaacgtaa gcgtcagttc cgtcaactgt
     3901 ggattgcgcg tatcaacgca gcagcacgtc agaacggtat ttcttacagc aaattcatca
     3961 atggcctgaa aaaagcctct gttgaaatcg accgtaagat cctggctgat atcgcagtat
     4021 tcgacaaagt agcgttcacc gctctggttg aaaaagcgaa agcagctctg gcataagcca
     4081 gttgaaagag ggagctagtc tccctctttt cgtttcaacg ccatcaaaac attgactttt
     4141 atcgccgtag ccttttcaat aaaggtcttt tgaagagtac caaaaggtaa cgcaagcaat
     4201 gaatgctgct attttccgct tcttttttta ctttagcacc tgaatccagg aggctagcgc
     4261 gtgagaagag aaacggaaaa cagcgcctga aagcctccca gtggaggctt tttttgtatg
     4321 cgcgttttga aaaataaaaa gccagtctat caggcgtaat tgtcgcagcc agtctggaca
     4381 cggccagtgc ggagaaaccg gagcgtactt aagtacgtga ggatttcgag cactgcccgg
     4441 gaccaaaatg gcaagtaaaa tagcctgatg ggataggctc taagtccaac gaaccagtgt
     4501 caccactgac acaatgagga aaaccatgtc acatctcgca gaactggttg ccagtgcgaa
     4561 ggcggccatt agccaggcgt cagatgttgc cgcgttagat aatgtgcgcg tcgaatattt
     4621 gggtaaaaaa gggcacttaa cccttcagat gacgaccctg cgtgagctgc cgccagaaga
     4681 gcgtccggca gctggtgcgg ttatcaacga agcgaaagag caggttcagc aggcgctgaa
     4741 tgcgcgtaaa gcggaactgg aaagcgctgc actgaatgcg cgtctggcgg cggaaacgat
     4801 tgatgtctct ctgccaggtc gtcgcattga aaacggcggt ctgcatccgg ttacccgtac
     4861 catcgaccgt atcgaaagtt tcttcggtga gcttggcttt accgtggcaa ccgggccgga
     4921 aatcgaagac gattatcata acttcgatgc tctgaacatt cctggtcacc acccggcgcg
     4981 cgctgaccac gacactttct ggtttgacgc tacccgcctg ctgcgtaccc agacctctgg
     5041 cgtacagatc cgcaccatga aagcccagca gccaccgatt cgtatcatcg cgcctggccg
     5101 tgtttatcgt aacgactacg accagactca cacgccgatg ttccatcaga tggaaggtct
     5161 gattgttgat accaacatca gctttaccaa cctgaaaggc acgctgcacg acttcctgcg
     5221 taacttcttt gaggaagatt tgcagattcg cttccgtcct tcctacttcc cgtttaccga
     5281 gccttctgca gaagttgacg tcatgggtaa aaacggtaaa tggctggaag tgctgggctg
     5341 cgggatggtg catccgaacg tgctgcgtaa cgttggcatc gacccggaag tttactctgg
     5401 tttcgccttc gggatgggga tggagcgtct gactatgttg cgttacggcg tcaccgacct
     5461 gcgttcattc ttcgaaaacg atctgcgttt cctcaaacag tttaaataag gcaggaatag
     5521 attatgaaat tcagtgaact gtggttacgc gaatgggtga acccggcgat tgatagcgat
     5581 acgctggcga atcaaatcac tatggcgggc ctggaagttg acggtgtaga accggttgcc
     5641 ggtagcttcc acggcgtggt cgttggtgaa gtggttgagt gtgcgcagca tccgaacgct
     5701 gacaaactgc gtgtgacaaa agtaaatgtc ggcggcgatc gcctgctgga tatcgtctgc
     5761 ggtgcgccaa actgccgtca gggcctgcgt gtggcggtag ctaccattgg tgctgttctg
     5821 ccgggtgatt tcaaaattaa agcggcgaaa ctgcgtggcg aaccgtctga agggatgctg
     5881 tgctcctttt ctgagctggg aatttctgac gatcacagtg gcattatcga actgcctgcg
     5941 gatgcgccga ttggcaccga catccgcgaa tacctgaaac tcgatgataa caccatcgaa
     6001 atcagcgtga caccaaaccg tgccgactgc ttaggtatca ttggtgttgc gcgtgacgtt
     6061 gccgtgctga atcagctgcc gctggttgaa ccggaaatcg ttccggttgg tgcgaccatc
     6121 gacgacacgt tgccgattac agtcgaagcg ccggaagcct gcccgcgcta tcttggccgt
     6181 gtggtaaaag gcattaacgt taaagcgcca actccgctgt ggatgaaaga aaaactgcgt
     6241 cgttgcggga tccgttctat cgatgcagtt gttgacgtca ccaactatgt actgctcgaa
     6301 ctgggccagc cgatgcacgc tttcgataaa gatcgcattg aaggcggcat tgtggtgcgg
     6361 atggcaaaag agggcgaaac gctggtgctg cttgatggca ctgaagcgaa gctgaatgct
     6421 gacactctgg tcatcgccga ccacaacaag gcgctggcga tgggcggcat ctttggtggc
     6481 gaacactctg gcgtgaatga cgaaacgcaa aacgtcttgc tggaatgcgc tttcttcagc
     6541 ccgctgtcta tcaccggtcg tgctcgtcgt catggcctgc atactgatgc gtctcaccgt
     6601 tatgagcgtg gcgttgatcc ggcactgcag cacaaagcga tggaacgtgc gacccgtctg
     6661 ctgatcgaca tctgcggtgg tgaggctggc ccggtaattg atatcaccaa cgaagcaacg
     6721 ctgccgaagc gtgcaaccat cactctacgt cgtagcaaac tggatcgcct gataggccat
     6781 catattgctg atgagcaggt aactgacatt ctgcgtcgtc tcggctgcga agtgaccgaa
     6841 ggcaaagacg aatggcaggc agttgcgccg agctggcgtt tcgatatgga gattgaagaa
     6901 gatctggtcg aagaagtcgc gcgtgtttac ggctacaaca acatcccgga tgagccggta
     6961 cagacaagcc tgattatggg tactcaccgt gaagctgacc tgtcgctcaa gcgcgtgaaa
     7021 acgctgctca acgacaaagg ctatcaggaa gtgatcacct acagcttcgt tgatccgaaa
     7081 gtgcagcaga tgatccatcc aggcgttgaa gccttactgc tggcaagccc gatctctgtt
     7141 gaaatgtcag caatgcgtct ttctctgtgg accggcctgc tggcaaccgt ggtgtacaac
     7201 cagaaccgtc agcagaaccg tgtgcgcatt ttcgaaagcg gtctgcgttt cgtaccagat
     7261 actcaggcac cgttgggtat tcgtcaggat ctgatgttag ccggtgtgat ttgcggtaac
     7321 cgttacgaag agcactggaa cctggcaaaa gagaccgttg atttctatga tttgaaaggc
     7381 gaccttgaat ccgttctcga cctgaccggt aaactgaatg aggttgagtt ccgtgcagaa
     7441 gcgaatccgg cactgcatcc ggggcaatcc gcagcgattt atctgaaagg tgaacgtatt
     7501 ggttttgttg gagttgttca tcctgaactg gaacgtaaac tggatcttaa cggtcgcact
     7561 ctggtgttcg aactggagtg gaacaagctc gcagaccgcg tggtgcctca ggcgcgcgag
     7621 atttctcgct tcccggcgaa ccgtcgtgac atcgcggtgg tggtcgcaga aaacgttccc
     7681 gcagcggata ttttatccga atgtaagaaa gttggcgtaa atcaggtagt tggcgtaaac
     7741 ttatttgacg tgtaccgcgg taagggtgtt gcggaggggt ataagagcct cgccataagc
     7801 ctgatcctgc aagataccag ccgtacactc gaagaagagg agattgccgc taccgtcgcc
     7861 aaatgtgtag aggcattaaa agagcgattc caggcatcat tgagggattg aacctatggc
     7921 gcttacaaaa gctgaaatgt cagaatatct gtttgataag cttgggctta gcaagcggga
     7981 tgccaaagaa ctggttgaac tgtttttcga agagatccgt cgcgctctgg aaaacggcga
     8041 acaggtgaaa ctctctggtt ttggtaactt cgatctgcgt gataagaatc aacgcccggg
     8101 acgtaacccg aaaacgggcg aggatattcc cattacagca cggcgcgtgg tgaccttcag
     8161 acccgggcag aagttaaaaa gccgggtcga aaacgcttcg cccaaagacg agtaatctga
     8221 tctaactaaa aaggccgcta tgcggccttt tttcttttca ctgtcgaaga gtcaccgtaa
     8281 aatcaacgcc atgacacttc agcagaacgg ataccatgct gacacttgcc cgccaacaac
     8341 agcgacaaaa tattcgctgg ttattatgcc tgtcagtttt gatgctgctg gcgcttctct
     8401 taagcctttg cgccggtgaa caatggattt cgccaggtga ctggtttact cctcgtggcg
     8461 aactgttcgt ctggcaaatt cgcctgccac gtacgctggc tgtattgctg gttggtgcgg
     8521 cgctggctat atccggcgct gtaatgcagg cgttgtttga aaatcctctg gcagaacctg
     8581 gactacttgg cgtctctaac ggcgcaggcg tggggcttat cgccgcggta ttgcttgggc
     8641 aagggcaact ccccaactgg gcgctagggc tgtgtgcgat tgctggcgcg cttatcatca
     8701 ctttaatact cttacgtttc gcccgtcgtc atctttcgac cagtcggtta ttgctggctg
     8761 gcgttgcatt agggattatc tgcagcgcac taatgacgtg ggctatctac ttttccacct
     8821 ctgttgattt acgtcagctg atgtactgga tgatgggcgg ttttggcggc gtagactggc
     8881 ggcaaagctg gctgatgctg gcattgatcc ccgtgttgtt gtggatctgt tgtcagtcca
     8941 ggccgatgaa tatgttagca cttggcgaga tctcggcgcg gcaactgggt ttacccctgt
     9001 ggttctggcg caatgtgctg gtggcagcga ccggctggat ggttggcgtc agtgtggcgc
     9061 tggcgggtgc tatcggcttt attggtctgg tgatcccaca tattcttcgg ttgtgtggtt
     9121 taaccgatca tcgcgtatta cttcccggct gcgcgctggc aggggcgagc gcattgctgc
     9181 tggccgatat tgtagcgcgc ctggcattag ctgccgcaga gctgcctatt ggcgtggtca
     9241 ccgcaacgtt gggtgcgccg gtgtttatct ggttattgtt aaaagcagga cgttagccgc
     9301 aaaaagacgg tctatgatta aaagctaaga tttttactga ccacacccag gagaaacgat
     9361 gcaagattcc attctgacga ccgtagtgaa agatatcgac ggtgaagtga ccacgctgga
     9421 gaagtacgcc ggtaatgtgc tgttaattgt caatgtcgcc tcaaagtgtg gcttaacgcc
     9481 gcaatatgag cagttggaga atattcaaaa agcctgggcc gatcgaggtt ttgtggtgct
     9541 gggattcccg tgcaaccagt ttctggaaca agaaccgggc agcgatgaag agattaaaac
     9601 ttactgtacc accacatggg gggtgacgtt cccgatgttc agtaagattg aagttaatgg
     9661 cgaaggacgc catccgctgt atcaaaaatt gattgccgca gcgccgaccg cagtcgcgcc
     9721 ggaagagagc ggattctatg cccgtatggt cagcaaaggc cgtgcaccgc tgtacccgga
     9781 tgatatttta tggaattttg aaaaattcct ggttggcagg gacggaaaag tcatccagcg
     9841 tttttccccg gatatgacgc cggaagatcc cattgtgatg gaaagcatta aactggcgtt
     9901 ggcaaaataa tgtctattgt gatgcagtta caggatgttg cggaatctac ccgcctgggg
     9961 ccgctttcag gcgaggttcg ggctggggaa atcctgcacc tggtggggcc gaatggcgcg
    10021 ggtaagagta ccttactggc gcgaatggcc ggaatgacca gcggtaaggg aagcattcag
    10081 ttcgcggggc aaccactgga agcatggtcc gcaacaaaac tcgcgctgca tcgcgcctat
    10141 ctttcacaac agcagacgcc gccgtttgca atgccggtct ggcactacct gacactgcat
    10201 cagcacgata aaacgcgtac cgaactactg aatgatgtcg caggggcgct ggctcttgat
    10261 gacaaactcg gtcgtagcac caatcaactt tccggcggtg aatggcaacg cgtacgtctt
    10321 gctgcggtgg tgttgcaaat cacaccacaa gccaatcccg caggccaatt gctgcttctt
    10381 gatgagccga tgaacagtct tgatgttgcg caacaaagtg tgttagacaa aattctgagc
    10441 gcgctgtgtc agcaaggact ggcgattgtg atgagcagtc acgatctcaa ccacacgttg
    10501 cgtcatgcgc atcgggcgtg gttgctaaaa ggtggaaaaa tgctggccag tggacgcaga
    10561 gaagaggtgc tcacgccgcc aaatctggcg caggcctatg ggatgaattt tcgccgtctg
    10621 gatatcgaag gtcacagaat gctgatttcg accatctgat ttcattagta tcttgaaaat
    10681 cgggtaatta cgacgctaaa ttaatgcaag aataaaaaac agaggaaccg tacggaatgc
    10741 gtttctgcct tattttgatc acagcactgt ttctggccgg gtgtagccac cataaagcac
    10801 cgccgccaaa tgccagactt tctgattcga ttaccgttat tgccggtttg aacgaccagc
    10861 tacaaagctg gcatggcacg ccgtatcgtt atggtggcat gacgcggcgc ggtgtggact
    10921 gttcgggatt tgtggttgtg acgatgcgcg atcgtttcga tttgcatctg ccccgtgaaa
    10981 ccaaacaaca agcctctatt ggcacgcaaa ttgataaaga cgagttgctg cctggtgacc
    11041 tggtcttttt caaaacgggt tccggacaaa atggtttgca tgtaggtatt tacgatacca
    11101 acaaccaatt tatccacgcc tctaccagca agggagtgat gcgttcctca cttgataatg
    11161 tctattggca gaaaaatttc tggcaggcga gacgaatcta gtatgtcgaa aaattgacgg
    11221 cgatgataca tcgccgtcaa tttactcctg aatacagtag tattttgcag acaaaaattc
    11281 cgtaaatgga ttattcgttt ataatcagga taatctaaaa aaaggccccc tgaaaaagtg
    11341 gttaacatgg aacaaatgaa agttactcct tctgtaattg ttatgtctta tggcctcatc
    11401 ccttttagcc ggatactgaa aaacatcctt cgagagggac ggttaccatg aagatttttt
    11461 tggagaatct ttatcattct gattgctatt tcctgccgat aagagataac cagcaactcc
    11521 ttgtcggcgt tgagttaatt acccatttct ccagtgaaga tggcacggtt cgtatcccga
    11581 cgagtcgggt tatagcgcaa cttaccgagg agcaacattg gcaattattt tcagagcagt
    11641 tggaattact aaaatcatgc caacactttt ttattcaaca taaactcttt gcctggctta
    11701 atttaacgcc acaagtggct acattattat tagacaggga taattacgca ggcgaactat
    11761 taaaatatcc gtttattgaa ctgttgatta atgagaatta cccgcatctc aatgaaggaa
    11821 aagataatcg cgatcttttg tccttatcgc aaatgtatcc ccttgtgtta ggcaatttgg
    11881 gagcaggtaa tagcacaatg aaagcggttt ttgatggtct tttcacccgc gtaatgctgg
    11941 agaaaggttt tattcagcaa caaatcacgc atcgctcctt cgaaccgttt attcgtgcga
    12001 tccaggcgca aatttcccct tgttgcaatt gcatcatcgc ggggggcatt gatacgccag
    12061 aaatattggc acagattacc cccttcgatt tccatgctct ccagggatgc ctgtggcctg
    12121 ccgttcctat aaatcagata acgacgttgg ttcagcgata accctcctgt ttgctggtgt
    12181 ttaagacgag agtaaccgtc tacactatca aacagatagg atctatgacc ctgtctttta
    12241 ttacccgctg gcgcgatgaa ttgccagaaa cctatacagc actttcccct acgcctttaa
    12301 ataatgcccg gctgatttgg cataataccg aactcgctaa cacgctgagt attccatcgt
    12361 cgctgtttaa aaatgccgca ggtgtctggg gcggcgaaac cctactgcct ggtatgtcac
    12421 cactggcaca ggtttacagt ggtcatcagt tcggcgtctg ggcgggccaa ctgggtgatg
    12481 ggcgcggcat tttacttggc gaacaactgc ttgctgatgg cactacaatg gactggcatc
    12541 tgaaaggtgc tggcctgacg ccttattcgc gaatgggtga tggacgggcg gttttacgtt
    12601 cgacgatacg agaaagtctc gccagtgagg cgatgcatta tctgggcatt ccgacgaccc
    12661 gcgcgttaag tatcgtcacc agcgattcgc cagtgtatcg ggaaacggtg gagccaggcg
    12721 cgatgctgat gcgtgtggca ccaagtcatc tgcgctttgg tcatttcgaa catttttact
    12781 atcgccgcga gccggaaaag gttcgtcagt tggctgactt tgccattcgt cattactggt
    12841 cacatcttga agacgatgag gacaaatacc gtctctggtt taacgatgtt gtcgcacgta
    12901 ccgcgtcgtt aattgcccaa tggcagacgg tcggctttgc acatggggtg atgaataccg
    12961 acaacatgtc gctgctggga ctgacgcttg attacgggcc gtttggtttt cttgatgatt
    13021 acgaacccgg ttttatttgt aatcactcgg atcatcaagg gcgttatagc tttgataatc
    13081 aacctgctgt cgcgttgtgg aatttacagc gtctggcgca gacattgtca ccatttgttg
    13141 ccgtagatgc cctgaatgag gccctggaca gctatcagca ggttttgttg acgcattatg
    13201 gacaacggat gcggcagaaa ctgggcttca tgacggagca aaaagaggat aacgcgctac
    13261 tgaatgaatt attcagtctg atggcgcgag agcgcagcga ttatacccgc acattccgca
    13321 tgctgagtct gaccgagcag cacagcgcgg cgtcaccgct acgtgatgag tttattgatc
    13381 gtgcggcatt tgatgactgg tttgcccgtt atcgggggcg tttgcaacaa gacgaggtta
    13441 gcgatagtga gcgtcagcaa ctgatgcaaa gcgttaaccc agctctggta ttgcgcaact
    13501 ggttggcgca acgggcgatt gaagcggcag aaaagggtga tatgatggaa ttgcaccgcc
    13561 tgcatgaggc gttgcgaaat cctttcagcg acagagatga tgactatgtc agtcgtccac
    13621 ctgactgggg taaacggctg gaagtcagct gctcgagtta aaggctactt agttaacagc
    13681 agcttgccag cctgagtttt acggagcaga tattcttgcc cgtcatgatc gataatcagt
    13741 ttgccatccg gacctaacag tgtctggctg gtaattcgcc ggatgggctg cggggaagcg
    13801 gtcttgtgat tggcatccgt ttcaggcgtg agttttctgc tatccgtata acgcataaaa
    13861 attaataata attgatataa caatgattct tattatcaat attatggcag gccagagcaa
    13921 gcagtaatgt gttgagaaga caacaagttg cgcagaaatg cccatcccat caggaatggg
    13981 cacgcattca gaagcgggta tctaccgcag aggcgagttt ttcgaccagg cgttcggtat
    14041 cttcccagcc cagacacggg tcggtaatgg attgaccgta agtgagcggc tgaccgccga
    14101 cgattttttg cgttccttcg cgcaggaaac tttccgccat aattccagca atctccgtag
    14161 agccattgcg gatttgctga caaatatcct cacaaacttc taactggcga cggtgctgct
    14221 tctggcagtt accgtggctg aaatccacca ccagatgttc aggtaaatca aactcgtgca
    14281 gcgtatcgca ggctgcggcg atatcatcgg catgataatt cggttttttg ccgccacgca
    14341 taataatgtg gccatacggg ttgccgctgg tctgatagat ggtcatctga ccatttttgt
    14401 ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat agcatccacg gcaatccgcg
    14461 tgttgccatc ggtaccattt ttaaaaccta ccggacagga gagtgctgaa gccatttcgc
    14521 ggtggatctg actttcggta gtacgagcgc caatcgcgcc ccaactgatt aaatcagcaa
    14581 taaactgacc ggtcaccata tcgaggaact cagtcgcggt agggacaccc agctcattta
    14641 cctgtaaaag taatttgcgc gccagctcca gaccgtgatt tacccgatag ctgccgttta
    14701 aatccggatc ggagattagc cctttccagc cgacaacagt tcgtggtttt tcaaaatagg
    14761 tgcgcattac gatttccagc cgtgactggt actggttgcg cagcgactgc agacgggtgg
    14821 cgtactccat tgcagcggtg agatcgtgga tcgagcaggg gccaataatg accaacagtc
    14881 gcttatcttc accattcagt attttttcaa ttctgcggcg ggagtcggtg acatgggtgg
    14941 cgacgccagg cgttacggga taccgtagcg cgagttcggc gggcgttacc aggctctcaa
    15001 tacgcgcagt acggagttcg tcagttctgt tcattacaag tctcagaaat ttgttgcttc
    15061 tacggcagga ctaccggaaa gtgatgcgca tcacgataaa ccaatcgcag ctgatttcaa
    15121 gtgcgaggtg tgtcacacct cgccgggagg ttagcatgat aacaaaaaaa caaaccaatg
    15181 cactagttct ctagtacatt cggcgactaa ggcccatgat atcgaggatc ttggtggcaa
    15241 tctcttctac cgaataattg gtactgttaa tccacgggat ctgattttta cggtacaagg
    15301 cttccacttc cgcgacttcc atcctgcact gacgaagcga ggcatagcga ctgttctccc
    15361 gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat agtcaggccg aacaatttat
    15421 gctgaagcgg tttgagcgac gcgggtagca ccagattatc catatcgtcg gcaataaagg
    15481 ggtagtttgc cgcgcggata ccaaattgca ttgccagata cagactggtg ggggttttac
    15541 cacagcgaga aacaccgagc aggatcacct gagcctgatc cagattgcgc aacgaaatgc
    15601 cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg cgcatcatat ttattgagat
    15661 tattagggtt aaggccatgg gtacgatgag caatcggcgt tggatccagt ttcatctctt
    15721 gttgtagcgg ggcaaccagc gcctgaacga tatcctggca aaagccttca ctttgcaaga
    15781 tgatggcgcg aatctccggc aacacgatgg agtagaagac cagcgggcgc acgcctgtct
    15841 ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc acggctctca ttttcgacaa
    15901 acggcagcgt gatgctgctg atagtgacgg gaaattgtga cattactgcg tgtcctaata
    15961 cctccgcagt tattgccgta ccatcagaaa tataaaaaac gtggcgatca acagcattat
    16021 ccattttgtt cttcccgtga tgcagacata attctatgaa agcataaatt aaaaacgcac
    16081 agaagcgtag gacgtaatgt ctggtttata aaatgaacct tcaattttat tttttatgaa
    16141 aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa attgtcgaat
    16201 agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat atttgcttga
    16261 acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca gaaatgtgtt
    16321 tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg ctcgtcaccg
    16381 ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg gggcaaaaat
    16441 gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt tccgaatggt
    16501 ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt aaaccagcgc
    16561 atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc gaaagcgggc
    16621 gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga aaacgccata
    16681 cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc ggtacgctcc
    16741 tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga aaccttcctc
    16801 aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc ttctctgttt
    16861 aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg cgtggcgctc
    16921 tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt gatgttctcc
    16981 attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg gggccttggg
    17041 gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa accgacactg
    17101 gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat ccgcatggtt
    17161 tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc gcaggaacag
    17221 cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca ggccgtacaa
    17281 attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg ccacaccggc
    17341 aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca ggtcatggag
    17401 cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat cggtcatcgc
    17461 atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg catcgaacct
    17521 ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat gaagaaagca
    17581 tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat cgctcgtgaa
    17641 ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa agacggtgag
    17701 aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga gttgctggaa
    17761 tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa agtgatgatg
    17821 aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga aggcgtgggc
    17881 cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg cgcactgctt
    17941 gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat gaaaggtttt
    18001 gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac gctgggtgcc
    18061 gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa cgaatatgcc
    18121 aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct cggcttccgt
    18181 ggcgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga gtgtgaagca
    18241 gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat cccgttcgtg
    18301 cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg gctgaaacgt
    18361 ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc cttgctggcc
    18421 gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat gacgcagctg
    18481 gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga gcgcaacgat
    18541 gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg caaatatgtc
    18601 gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt gatggaagag
    18661 gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt aagcctggct
    18721 gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta tgtgatcccc
    18781 gaagatgaaa cttattcaat ctcttcacag atatcctgcg ttaatcgccg tataatatct
    18841 tttcttaaca aaaacttttg tattttacct gaggcagtgc gcggtagttt ttcgattacc
    18901 acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa agccaccatc
    18961 tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca tgatcgttca
    19021 cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg atgctgcaat
    19081 aaaatatctt ccacttcacg gctgctaata tattcgccgc cgcggacaat aatatctttt
    19141 ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc gccgctgtaa
    19201 taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa ataccccata
    19261 aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa ggtcttgcgt
    19321 gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt atgcataaag
    19381 cgcgacaaag aatcatcgag attcaccacc gcatgcggcg aactttctgt ggaaccataa
    19441 acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt ggggattgtg
    19501 gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt ctctagtaaa
    19561 ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg ctgctgctca
    19621 agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg agcgccaatt
    19681 aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg cgcaggcatc
    19741 ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc actggcgaga
    19801 atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga ggtaaacagc
    19861 accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat attgtcggcg
    19921 ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac gccgacaatt
    19981 tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg acgcgtttgt
    20041 ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac ccacaccagt
    20101 tctgcttccc gctaggaagg caacagcggc acggaaaccg caccgatttt caggcaggca
    20161 agatggataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg atcgcctgat
    20221 tcaatgccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc gagcgcgcta
    20281 taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat cgcacgagcg
    20341 gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg acgatacgcc
    20401 gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg aaacagaaca
    20461 tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa tcaataccga
    20521 aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc acccgacagg
    20581 tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca tcctgcttat
    20641 aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg ggattttccg
    20701 tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct aatttgatgt
    20761 cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc catctttcag
    20821 caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta ccggctggtt
    20881 tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc gtgggtattg
    20941 gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct gcatatcacg
    21001 catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg cggagaaatc
    21061 cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg caatggctaa
    21121 atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt caccaacgat
    21181 catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg gcaccatatg
    21241 cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg ggtgttgttt
    21301 aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac ccaatccaca
    21361 aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc ccatcaggcc
    21421 gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa tattaaagcg
    21481 atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag cgtaatgatg
    21541 cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa cgccatcagc
    21601 cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag tgaccttgtt
    21661 tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc ccgcctgctc
    21721 ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag atgcgtgttg
    21781 cggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt cggttaagaa
    21841 ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa agcctggaat
    21901 gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac atccggcact
    21961 gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca gtgcggcaac
    22021 gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg acatgcgtta
    22081 tgctcccttc ccgctgcgtt tacagcggga gatgtggttg aaaggggaat tagcggctca
    22141 actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg tagtcggcat
    22201 agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt acgccgttgc
    22261 cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga tcggatttca
    22321 gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg ccttcggcaa
    22381 ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg accattttca
    22441 ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct ttgctcaggt
    22501 ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga cgcggagcaa
    22561 cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac ggttcctgaa
    22621 caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga gcgaacgcta
    22681 acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc accgccgtgg
    22741 catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga gctttaccac
    22801 gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg gcagcaatgc
    22861 tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca taaatacatt
    22921 ttggatcata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg gcatacacct
    22981 gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga ttatcgctaa
    23041 agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca gttcagggct
    23101 ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc gagaatgatg
    23161 tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag tggcgcgctc
    23221 tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc cgcgccgaga
    23281 atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca gagcacggct
    23341 ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat caccagtgta
    23401 ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag aatttctccg
    23461 accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa gatcagtaaa
    23521 tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag aggcagtgca
    23581 tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga tagcacgtct
    23641 ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc tatctcatcg
    23701 tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg gctgattttg
    23761 gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc ctgttcttca
    23821 ggcaccagct taaagcaggt tattattttc atggtttctc ctgtgagtcg catacggaac
    23881 catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt gcggggtttt
    23941 cgacggagca agctgttttc cttgccaaca atcttgcaaa aaaaatgcga tggctaaaac
    24001 aaaagtgcaa tttgcggcaa tatagttatt ccatgaccgt acaatcttgc attcagtgta
    24061 aataatcttg tttagctctc gctaaacgcg gttgtataac cgcaatacac gccgtatttt
    24121 atccggcata tattgttttt gcttgctcat ggaataggga ggttgtcatg tatcaacgct
    24181 gttttgataa tgccagtgaa acgctgtttg tcgccggtaa aacgccacgg ctttcacgtt
    24241 ttgcatttag cgatgatcca aaatgggagt ctggacatca cgttcatgac aatgaaaccg
    24301 agctgattta cgtcaagaaa ggggttgcaa ggtttaccat cgattcttcg ttatatgtcg
    24361 cacatgcgga tgacattgtg gtgatagaac gcggcaggct gcatgcggtg gcctctgacg
    24421 ttaacgatcc ggcaacgacg tgtacctgtg cgctgtacgg ctttcagttt cagggggctg
    24481 aggaaaatca gttactgcaa ccgcattctt gtccggtaat tgccgcgggg cagggaaaag
    24541 aagtcataaa aaccttattt aatgagctaa gtgtgatttt gccgcaaagt aaaaatagcc
    24601 aaacatcttc gttatgggat gcatttgcct atacattagc aattctttac tacgaaaact
    24661 ttaaaaatgc ttatcgttcg gagcaggggt atattaaaaa agatgttctg ataaaagata
    24721 ttcttttcta tctgaataat aattatcgcg aaaaaatcac tttagaacag ttatcgaaaa
    24781 aatttcgtgc cagcgtcagt tatatttgcc atgaatttac caaagagtat cgtatttccc
    24841 ctattaacta tgttattcaa cggcgtatga cggaagcaaa atggtcactc attaatactg
    24901 aattatcaca ggcagagatc tcctggcgtg tgggttatga aaatgtcgat cactttgcca
    24961 aactgttttt gcgccatgtc ggttgttcgc ccagcgatta ccgtaggcaa tttaaaaatt
    25021 gttttgcgga acaagaaatc ttatctgaat ttcctcaacc ggtaagtctt gccggataaa
    25081 tctggcggcg cactttgtta aaaagctgcg ccgcctgcag attatttgtt ctgatagtct
    25141 ttcaggatct gccgacctgc tacgtaaatc ataatttcgt ctgtaccgcc gccgatacgt
    25201 tcacaacgga catcacgcca gaagcgggag acgcgcgcct catcggtata gcccagaccg
    25261 cccatgattt gaatcgcatc atcaatgact tccattgcgg tacgtgcaca gtacagtttt
    25321 gccagcgccg cgctggtgcg cagtgattga tgctgatcgg cttgccatgc cactttcagc
    25381 accatgttgc gcatgttgtc gatcttaatc gccatcagcg ccagtttttc ctggatcatc
    25441 tggttatgcc caatgggttt accaaaagcg atacgctggt tggcatagcg agcggcatct
    25501 tcaaaggcac actcggcaaa gccggtgctg cgcgcggcgt tgatcaggcg ctccatctca
    25561 aagttgtaca tcacattgag gaaacccatt ccttcttcgc ccaccatgtc gctctcttca
    25621 acttccacgt tgtcgagata gacttcgcag gtgctgagca tatgccagcc gattttatgc
    25681 agcgggttaa tcttaatgcc gggcttactg gagtcgaccc accacagggt gaaggctttt
    25741 ttgggatctt tcggttgcgg atcgcgcgcc aacaccagca tatacgggta ctctttcgca
    25801 ccggtaataa aggttttctg tccgttgatg taaaccttgc cgtttttacg cgtgtaagtg
    25861 gtagtggcac tgttgttgtc tgagcctgcg cctggctccg tcaacgccag ggcataggcg
    25921 ggatcacccg tttccagtgt gctttctgcc gttttacgta gctgctctgc agaaccgaaa
    25981 cggcgcatac tgtgaataca ttgaccgttg gtaatcaaaa atgccggagc accgcatttt
    26041 gacacttcca tcagcgccag catctgggta acgtaatccg cagggatacc gccgaactct
    26101 tccggcacac caagcatgga aataccgtta tccgccagtg cccgcataaa ctcacgcggg
    26161 tatgttccgt tttgatcgca ggtgcgggaa tactcttccg gaaagttagt cgtaatcaac
    26221 tcgcgaatac tggccagcag cagttcttgt tcttcagtta aagaaaaatc tatcgtgata
    26281 ctcctgttaa tgagccgctt caggcaggac aaaacccatc gccgcatcga taaataatct
    26341 ttcgtccatc agtttgagtt ctggcgaaat cactggggtg aaatccattt tgtcgagaat
    26401 atctttttgt aaatcgacgc caggagcgat ttcgattaaa tgcaggccgt cttctttcag
    26461 cgtgaataca gcacgttcgg tgatgtaacg aacatccagt ccccgttcga gcgcgatttt
    26521 tccgctgaaa gtaatttccg gtagttcccg aataaatttc ttcacccgtc cttcctggac
    26581 gatatttaat ttgccgtcgg taatttctgt ttttaaactg cccg
//
LOCUS       DADLSA010000043        26311 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.043, whole genome shotgun sequence.
ACCESSION   DADLSA010000043 DADLSA010000000
VERSION     DADLSA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.043"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..1371
                     /locus_tag="JN691_001812"
     CDS             <1..1371
                     /locus_tag="JN691_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7243272.1"
                     /translation="DKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQ
                     EARQRYISNALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTEL
                     AKLKYQTAQGELKTLTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNE
                     AELLGYGQGERLRECMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYL
                     SERLKDQETFYAESDAQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNI
                     TEMLNGNKVEWRSWASSVLQEISKVLMNAAIVNGIRTAANSMSGAGGFIGSIGSWLGG
                     AVANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLG
                     VRAVGSMNGSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRD
                     GGMLSG"
     gene            1364..1705
                     /locus_tag="JN691_001813"
     CDS             1364..1705
                     /locus_tag="JN691_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7243273.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEATRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            1705..2403
                     /locus_tag="JN691_001814"
     CDS             1705..2403
                     /locus_tag="JN691_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAY7243274.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQSGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQLSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYMGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            2414..3157
                     /locus_tag="JN691_001815"
     CDS             2414..3157
                     /locus_tag="JN691_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7243275.1"
                     /translation="MTQTESAILAHTRRCAPAESCGFVISTPEGERYLPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGELPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFHREDDWWRNGQNLYLDNMVVT
                     GFYRVPLSSAQPGDILLCCFGASVANHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWNTSAFTGIYNDLAAASACM"
     gene            3055..3735
                     /locus_tag="JN691_001816"
     CDS             3055..3735
                     /locus_tag="JN691_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAY7243276.1"
                     /translation="MATTNAFCLASPPLEHICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRLQMNEGWYQIRIAGEDTAPEVVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSAMAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            3976..7444
                     /locus_tag="JN691_001817"
                     /pseudo
     CDS             3976..7444
                     /locus_tag="JN691_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            7513..8136
                     /locus_tag="JN691_001818"
     CDS             7513..8136
                     /locus_tag="JN691_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY7243277.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAESGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTVHLGGIVALSYASVKKSDTMKTGENTFHYESLHG
                     RYVSLMAGPVWQLSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVEMVVIDLAYEGSGSGDWRTDGFIVGIGYKF"
     gene            8201..11014
                     /locus_tag="JN691_001819"
     CDS             8201..11014
                     /locus_tag="JN691_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7243278.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSASEAATRATDAADSARAANTSAG
                     QAATSAQEAFSSAGTASAKASEASKSAAAAESSKSAAATSAGAAKTSETNAAASVTAA
                     EKSAESAASSASTATTKAGEATEQASAAARSASAAKTSETNAKASETRAESSKTAAAS
                     SASSAASSASSASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAA
                     TRAETAAKRAEDIASAVALEDANTTKKGIVQLSSATNSTSETLAATPKAVKAANDNAN
                     GRVPSNRKVNGKALTADITLTPKDIGTLNSVTISFSGGAGWFKLATVTMPQASSIVYI
                     ALIGGAGYNVVSPQQAGISELVLRAGNGNPKGITGALWKRTAVGLTNFAWINTSGDTY
                     DIYVEIGNYATSVNIHWDCTTNASVSIYTSPTYSASKPSSVTDGVVYTMYSSHQKPTP
                     SDIGALPTTGGTISGPLSVTDGITGALKGNADTATKLAAAPKINGVKFDGSADINLTP
                     ENIGAFARRSTGAYADSDGAVPWNAESGAYNVTRSGDSYILVNFYTGVGSCRTLQMKA
                     HYRNGGLFYRSSRDGYGFEEDRAEVYTSKNLPPESYPVGAPIPWPSDTVPSGYALMQG
                     QTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASST
                     DLGTKTTSSFDYGTKSSNATGDHNHSRGTMEITGTLGYFRSDSGNFYTASEAFTLGGS
                     APAHGFTGSQFTYGVPVNFNASRTWSGATSTTGNHAHSVPIGAHAHSVAIGSHGHTIT
                     VNAAGNAENTVKNIAFNYIVRLA"
     gene            11014..11598
                     /locus_tag="JN691_001820"
     CDS             11014..11598
                     /locus_tag="JN691_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7243279.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPLDIPAGFVAVFNSDESSWHLFEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIRDAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(11714..11962)
                     /locus_tag="JN691_001821"
     CDS             complement(11714..11962)
                     /locus_tag="JN691_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY7243280.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADAWFVH"
     gene            complement(12024..13121)
                     /locus_tag="JN691_001822"
                     /pseudo
     CDS             complement(12024..13121)
                     /locus_tag="JN691_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            13210..14247
                     /gene="dusA"
                     /locus_tag="JN691_001823"
     CDS             13210..14247
                     /gene="dusA"
                     /locus_tag="JN691_001823"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAY7243281.1"
                     /translation="MHDNHETQKINQTSVMPEKTGVYWNSRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFLGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            14381..14623
                     /gene="pspG"
                     /locus_tag="JN691_001824"
     CDS             14381..14623
                     /gene="pspG"
                     /locus_tag="JN691_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAY7243282.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(14789..15772)
                     /locus_tag="JN691_001825"
     CDS             complement(14789..15772)
                     /locus_tag="JN691_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAY7243283.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            15855..17270
                     /locus_tag="JN691_001826"
     CDS             15855..17270
                     /locus_tag="JN691_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAY7243284.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            17323..18402
                     /gene="alr"
                     /locus_tag="JN691_001827"
     CDS             17323..18402
                     /gene="alr"
                     /locus_tag="JN691_001827"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY7243285.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRGAMKYVD"
     gene            18655..19848
                     /gene="tyrB"
                     /locus_tag="JN691_001828"
     CDS             18655..19848
                     /gene="tyrB"
                     /locus_tag="JN691_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAY7243286.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(20071..20613)
                     /locus_tag="JN691_001829"
     CDS             complement(20071..20613)
                     /locus_tag="JN691_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005059113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243287.1"
                     /translation="MVINFITRDGDDDMNISYVNSNKTTSLPVELDALNNKDISYAKD
                     FSYAKDFFLYIETQLKIAKDFCRPREEVSSSIASKVFHAFIDLVNKIRGKKDFMYICT
                     LCCFAEEVKGDYSHYRTFLFDIGNQYKVKLTQSGKKEFSLTLEFNDTIIESQKVTGNK
                     AKHILEDIEIFYRNKPDTYY"
     gene            20976..21689
                     /locus_tag="JN691_001830"
     CDS             20976..21689
                     /locus_tag="JN691_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B acid phosphatase"
                     /protein_id="HAY7243288.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            21800..22216
                     /locus_tag="JN691_001831"
     CDS             21800..22216
                     /locus_tag="JN691_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAY7243289.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            22220..22576
                     /locus_tag="JN691_001832"
     CDS             22220..22576
                     /locus_tag="JN691_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY7243290.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(22611..25433)
                     /gene="uvrA"
                     /locus_tag="JN691_001833"
     CDS             complement(22611..25433)
                     /gene="uvrA"
                     /locus_tag="JN691_001833"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAY7243291.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTSGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            25687..26223
                     /gene="ssb1"
                     /locus_tag="JN691_001834"
     CDS             25687..26223
                     /gene="ssb1"
                     /locus_tag="JN691_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAY7243292.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
BASE COUNT         6381 a         6467 c         7282 g         6181 t
ORIGIN      
        1 gataaacagc agagtgccct gacaaaaaca gagcgtgagc tggccctgtc cagactgaaa
       61 ggggaagaaa aagagcgtgt ccgactgggg tatgcggcgg atgacctcgg ttttgtgggt
      121 gatccgtatc aggaggcgag acaacgttat atcagtaatg ccctggaagc ctggcgcaat
      181 aacgaggcga ataaacccaa atcccggggt ggaaaatcag agacggaaaa agcggaagac
      241 agtttttccc ggctgctgaa gcagcagaaa gagcaattgg cacttgcggg gcagaataca
      301 gagctggcga agctgaaata ccagactgcg cagggcgaac tgaaaaccct gacggagatg
      361 cagaagcagg aactgctgcg caatgcggcc ctgattgacc agcaaaaaat ccgggagcag
      421 ttgcggtccc gggaagagac cctgaagaat gataatgtgg ctgcgcgtgc atcaaatgaa
      481 gcggaactgc tggggtacgg gcagggagag cggctccgtg aatgcatgcg ggagttgcag
      541 cagatccgcg acagtttccg ccagaaggat gcggaccttc agtctcagta tcagaccggg
      601 gatatcagtg aggattttta cagacaggct ctggcacaga acgcgcagta tctgagtgaa
      661 cgtcttaaag accaggaaac tttttatgcc gaatcggatg cgcagcgtgc ggactggcag
      721 aaagggctgc aggaggggtt cagtaactgg gtggataatg cgtccgatta cgcctcacag
      781 gcagcacagc ttgcgacgga gggtatctca gggatggtga ataacatcac ggagatgctg
      841 aacggaaata aagtggaatg gcgcagctgg gcctcatcag tgctgcagga aatatcaaaa
      901 gttcttatga atgccgcgat tgtcaacggg atcaggacgg cggcaaacag tatgtccgga
      961 gcgggaggat ttatcggcag tattggtagc tggctgggcg gtgcggtggc caatgcaaaa
     1021 ggcggcgtgt atacctcggc aaacctgagt gcgtacagca acagtattgt ggatacgccc
     1081 acgtactttg cctttgcaaa aggggcggga ctgatggggg aggccggtcc tgaagccatt
     1141 atgcccctga cccgggcggc ggatggctcg ctgggtgtgc gagcggtggg cagtatgaac
     1201 ggcagtgcag gtctggtgta ttccccggtc taccatatcg ccattcagaa tgacgggact
     1261 aatggccaga tagggccgga agctgcgggc agccttgtgc aactgattga ccagcgggtg
     1321 caggcggtga tgctgtccat gcgacgtgac ggaggaatgc tgagtggctg agataaaaac
     1381 gctgcatctg gtcccgcgtg aagggatgca ggtgagtgag aaaccgtcgg tggcgagggt
     1441 acggtttggt gacggttatg aacagcgccg cccgacggga cttaatgccc gactgaagac
     1501 gtttcaggcg gtgttccggg tgacggatga ggcgacccgg cgatggctgg aagagttttt
     1561 atcgtggcat ggtggttacc gtgccttttt gtggcgaccg ccgaaacata accggacggt
     1621 gagggtggta tgccgggagt ggagcgtcac agataacgcc aggtacagtg atttcagttg
     1681 tacgattgag caggtggtga actgatgcag gatattcacg aagaaagtct gaacgagtcg
     1741 gttaaatcag agcagtcacc gcgggtggta ctctgggaaa tcgacctgac ggtacagagc
     1801 ggtgagcggt attttttctg taatgagctg aatgaaaaag gggagccggt cacctggcag
     1861 gggcgtaagt atgaggcgta cccgattgat ggcagcggct ttgaaatgaa cgggaaaggc
     1921 agcagtgccc gcccgtcgct gacggtgtcc aatctgtttg gccttgtcac cgggatggcg
     1981 gaggatttgc agagtctggt gggtgccacg gtggtccgtc gccgggtgta tgcgcgtttt
     2041 ctggatgcgg tgaattttgt ggcgggcaat ccggaagcgg acccggaaca ggaactgagc
     2101 gaccgctggg tggtggagca gttatcagag ctgacggcca tgacggcctc gtttgtgctg
     2161 gcgacaccga cggagacgga cggggcgctg tttcccggtc gcatcatgct ggcgaacacc
     2221 tgtatgtgga cctaccgctc tgatgagtgt ggttacatgg gcggggctgt ggcggatgag
     2281 ttcgataaac ccaccacgga tatccgtaag gacagatgca gcaagtgcat gcgcgggtgt
     2341 gaactgcgca ggaatgtcgg caattttggc ggtttccttt ccattaataa actttcgcag
     2401 taaatcccgg tttatgacac agactgaatc agcgattctg gcacacaccc ggcgatgtgc
     2461 gcctgcggag tcttgcggct tcgtgataag cacgccggag ggggagcggt atctgccgtg
     2521 tgtgaatatc tccgcagagc cggaggcgta ttttcgtatc gcaccggaag actggctgcg
     2581 ggcagagatg cagggggaga ttgtggcact ggtccacagt catcccggtg agctgccctg
     2641 gctgagcgag gctgaccggc ggctgcagat aaaaagcgca ctgccctggt ggctggtctg
     2701 ccggggggaa attcataaat tccgctgtgt gccacatctg acaggacggc gctttgagca
     2761 cggggtgacg gactgttaca cgctgttccg ggatgcatac catctggcgg gaattgatat
     2821 gccggatttt catcgcgagg atgactggtg gcgcaacggc cagaacctgt acctggacaa
     2881 tatggtggtc accggctttt accgggtgcc cctgtcctct gcacagccgg gcgatatcct
     2941 gctgtgctgc ttcggcgcat cggtggctaa tcatgccgcc atttactgcg gcaacggtga
     3001 actgcttcac catattcctg aacaactgag taaacgggag aggtattccg aaaaatggca
     3061 acgacgaacg cattctgtct ggcgtcaccg ccactggaac acatctgcct tcacggggat
     3121 ttacaacgat ttggccgccg cctcagcctg tatgtgaaca cggcagcgga agccattcgc
     3181 gccctgtcga tgcagatgcc gggctttcgc cttcagatga acgaaggctg gtaccagata
     3241 cgtattgccg gtgaagacac ggcaccggag gtggtgtacg cccgccttca cgaacagctg
     3301 ggtgagggaa cggtcatcca cattgtgccg cgactggccg gggccggaaa gggtggactg
     3361 cagattgtgt tgggggcggc agccatcgtg ggctctttct tcactgccgg ggcatcaatg
     3421 gcgttatggg gttcagccat ggcagccggt ggtttttctg ccaccacgat gctgttttca
     3481 cttggagcca gcatgattct gggcggtgtg gcccagatgc tggccccgaa ggcaaaaaca
     3541 ccggattacc gcgcaacgga taacggcaga cagaacacgt acttttcctc gctggataac
     3601 atgattgccc aggggaaccc gatgccggtg ccttacgggg aaatgctggt tggctcccgc
     3661 cgtatatccc aggacatcag cacccgtgat gaaggcgggg gcggaaaggt cgtggttatc
     3721 gggcggcagg gataaaacat aaaaaaatcc cgcagtgatc gcggagctgc ggggacagac
     3781 aaatgaagat caatgttaag gagttgtttt tgttactcgg gcaaaaaaac actaacgcag
     3841 cgaaattata agcgccacag tcagtgtgtg aaaatgtgaa gatattcaga aattttattc
     3901 cgtcatgacg caggcacccg gtgaggtgcc tgttgttttt gtgagtgaac aattatcacg
     3961 gtaagaggtg atgtaatggg caaaggtggc ggcagggcgc acacaccgcg tgaggcgaaa
     4021 gacaatctca aatccacgca gatgatgagc gtgattgatg cgattggtga gggaccgata
     4081 gaaggcccgg tgaaaggcct gcagagtatt ctggtgaaca aaaccccgct gacggacacg
     4141 gacggtaatc ccgtgataca cggtgtgacc gcggtctggc gtgccgggga gcaggagcag
     4201 acaccaccgg aaggctttga gtcctctggc tctgaaactg tactgggtgt cgaagtgacc
     4261 agggcaaaac cggtaacacg caccattacg tcagcgaaca ttgaccgcct gcgggtgacc
     4321 ttcggggtgc agtcactggt ggagaccacg tcaaagggtg accgtaatcc gtcctctgtc
     4381 cgtctgctga ttcagttaca gcgtaacggt aactgggtga cggaaaagga tgtcaccatt
     4441 aacggcaaga ccacctcaca gttccttgcg tcggtgattc tggataatct gcctccccgc
     4501 ccctttaaca tccggatggt cagggagacg gcggacagca ccacggacca gctgcagaac
     4561 agaacgctgt ggtcgtcata caccgaaatc atcgatgtga aacagtgcta cccgaacacg
     4621 gccattgtgg ggatgcaggt ggatgcggag cagtttggtg gtcagcagat gacggtgaac
     4681 taccatatcc gcggtcgcat catccaggtg ccgtcaaact atgacccgga aaaacgcacg
     4741 tacagtggta tctgggacgg cagtctgaaa ccggcataca gcaacaatcc ggcctggtgt
     4801 ctgtgggaca tgctgactca cccgcgctac ggcatgggaa aacgtctggg ggcggcggat
     4861 gtggacaagt gggcgctgta tgccatcggg cagtactgcg accagatggt gccggatggc
     4921 ttcgggggca ccgagccgcg gatgaccttc aatgcgtacc tggcacaaca gcgtaaggcg
     4981 tgggatgttc tcagtgattt ctgctcggcg atgcgatgta tgccggtatg gaacggtcag
     5041 acgctgacgt tcgttcagga ccgcccgtcg gatgtggtgt ggccgtacac cagcagcgat
     5101 gtggtggtgg atgataacgg cgtggggttc cgctacagct tcagtgccct gaaggaccgg
     5161 cacacggcgg tggaggtgaa ttacaccgac ccgcagaacg gctggcagac ctccacggaa
     5221 ctggtggaag acccggaagc catactgcgc tacgggcgca acctgctgaa gatggatgcg
     5281 ttcggctgca ccagtcgcgg tcaggcccac cgtgccggac tgtgggtgat aaagaccgga
     5341 ctgctggaaa cgcagacggt ggatttcacg ctcgggtcac aggggctgcg tcacacaccc
     5401 ggtgacatta ttgaaatctg tgataacgac tatgccggga ccatgaccgg cggacgtgtc
     5461 ctgtccatcg atgccgccac ccgcaccctg acgctggacc gtgaggtgac actgccggag
     5521 accggtgccg ccacggtgaa cctgattaac ggcagcggta agccggtgag tgtggacatc
     5581 accgcacacc ccgcgccgga ccggatacag gtcagtaccc tgccggatgg cgtggagaca
     5641 tacggtgtgt ggggactctc cctgccgtca ttgcgccgtc gcctgttccg ctgtgtctcc
     5701 gtccgggaaa acacggacgg cacctttgcc atcacggcag tgcagcatgt gccggagaaa
     5761 gaagccatcg tggataacgg ggcgcacttt gacggcgacc agagcgagcg tcatccctcc
     5821 ggcagtgcag cacctgacgg tggaggtgag cgcagctgac ggccagtatc tggcgcaggt
     5881 gaaatgggac acgccgcggg tggtgaaggg cgtgcgcttc agtctgcgac tgaccagcgg
     5941 aagcggagaa ggcagccatc tggtgaccac cgccatcact gcggatacag agcatcgttt
     6001 cagtggtctg ccgctcgggg aatacaccct gacagtcagg gcaattaaca gttatggcca
     6061 gcagggcgaa ccggccacca ccaccttccg gattaacgcg ccagcaaaac ccgccaccat
     6121 tgagctgacg ccggggtatt ttcagataac ggcggtccca cgtcttgcgg tgtatgaccc
     6181 gacggtacag tttgaattct ggttctcaga aaaacgcatc acgaacacag cacaggtgga
     6241 aaaatctgcc cgttatctgg ggaccggcag tcagtggact gtctccggcc cgcacattaa
     6301 gcccgggaag gatttctggt tttatgtacg cagcgttaac ctagtgggga aatctgcttt
     6361 tgttgaagcc agtggacggg ccagcaatga tgcagaaggg tatctggggc tgtttcggga
     6421 aaaaatagga aaactgcatc tggctcaggg gctgtgggag ctgatagaca acagccagct
     6481 tgcggatgag atggcggaga tgaagaccac catcaccgaa acccgcaatg aaatcacaca
     6541 gacggtcagt aaaacgctgg aagaccagag cgccaccatt cagcagatac agcgcgtgca
     6601 gaaggacaca aatgatgacc tggctgcgct gtacatgctg aaggttcaaa aaacgaaaga
     6661 cggcattccc tatgtggccg ggattggtgc agggattgag gatactgatg gccagccact
     6721 gagcaacata ctgctgctgg ctgaccgtat cgcgatgata aatccggaga acggcaacag
     6781 cacgccgtta tttgtggcgc aggggaatca gctgttcatg aacgacgtgt tcctgaagcg
     6841 actgtttgcg gtgagtatca cgtcatccgg caaccccccg acgttttccc tgacgccgga
     6901 agggaagctg acagccagga acgcggatat cagcggagca attaccgcga ataccggcac
     6961 gctcaataat gtcaccatta acgagaactg tgtcatcaga gggaaactgt ctgcaaacca
     7021 gattgaaggc gacctggtga agacggtggg gaaagccttt ccccggaata acagttatgc
     7081 cagcgggacg gtaactgtca cagtttacga tgaccaggga ttcgaccggc agattatcat
     7141 tcccccggtg ctgtttcgcg gggcgaaaca ccagaatttc aacagcccga atcagcagtc
     7201 gtactggtat tccacctgta agctgcaggt gctgaagaac ggggttgaga ttttccatga
     7261 accggcaacg gatgtcagcc gggtgttctc atcggtgata gatatgccgg cagggcaggg
     7321 tcatgtcacc ctgacgttta atgtgtcgtc ggccggtgcg aacaactgga cgccgacaac
     7381 gtacatcagt gatttactgg ttgtggtcat gaaaaaatcc acggcaggga tcagtatcag
     7441 ctgacggttt attaacccgg acgggcacct caggaggtgc cttttttatt gactgaaaac
     7501 aaagaggtaa tcatgcggca tttatacgca acgatattat tgtttactac cctgctggca
     7561 ggaattgcct ttcctgcaca ggctgaaagc ggacacggtg cattttccgt gggatatgct
     7621 caggttcacc cgggcggcgt accggcattg tccggtaccg gtgcgcgtgc aggtgattta
     7681 aaagggatta atgtgaaata ccgttatgag ttcacggttc acctgggcgg cattgtcgcg
     7741 ctgagttatg catcggtgaa gaaaagtgac acgatgaaga cgggtgaaaa taccttccat
     7801 tatgaaagcc tgcacggtcg ttatgtcagt ctgatggccg gccctgtctg gcagctcagt
     7861 gagcgggtca gtctctatgg catggccggg atggcgtaca cccgctggtc tgacagtgtt
     7921 caggattacc ggcgtgatga agtaaaaccg gggtatgtga aggagaccac caccgccagt
     7981 gatggtcata ctgcgcgtca tctgtcgccg gcctggaatg ccgggattca gttcagtccc
     8041 gtagagatgg tggttattga ccttgcttat gaaggttccg gcagtggcga ctggcgcact
     8101 gacggtttca tcgtgggtat cggttataaa ttctgattag ccaggtaaca cagtgttatg
     8161 acagcccgcc ggttcaggcg ggcttttttg tggggtgaat atggcagtaa agatttcagg
     8221 tgtactgaaa gacggcacag gaaaaccggt acagaactgc acaatccagc tgaaagcaaa
     8281 acgtaacagc accacggtgg tggtgaacac gctggcatct gaaaatccgg atgaagccgg
     8341 gcgttacagc atggacgttg agtacggtca gtacagcgtt attctgttgg tggaagggtt
     8401 cccgccgtca catgccggga ccatcaccgt gtatgaagat tctcaacccg gtacgctgaa
     8461 tgattttctt ggtgccatga ctgaggatga tgtccgtccg gaggcactgc gccgttttga
     8521 actgatggtg gaagaggtgg cgcgtaacgc gtccgcagta gcacagaaca cggcagccgc
     8581 gaagaagtca gccggcgatg ccggcacatc tgccagtgag gcggcaaccc gtgcgactga
     8641 tgctgcagat tcagcacgtg cagccaacac gtcagccgga caggccgcga cgtcggctca
     8701 ggaggctttt tccagcgcag gaacggcatc agcaaaagcc tctgaggcat cgaaaagtgc
     8761 tgctgctgca gagtcctcaa aaagcgcggc ggccaccagt gccggtgcag cgaaaacgtc
     8821 agaaacgaat gcggcagcca gcgttaccgc cgctgaaaaa tcggcagaaa gcgccgcatc
     8881 gtccgcttca acagccacaa cgaaggctgg cgaagccact gaacaggcca gcgcagcagc
     8941 gaggtctgct tccgcagcga agacatccga aacgaacgcg aaagcgtcgg aaacccgtgc
     9001 agaatcctca aaaacggctg ccgcatcatc cgccagttcg gcggcgtcat cggcatcatc
     9061 tgcgtctgct tcaaaagatg aggcgaccag acaggcgtca gcagcgaagg gcagcgccac
     9121 gacggcatcc acaaaggcga cagaggctgc tggcagtgcg acagcggcag cacagagcaa
     9181 aagtacggca gaatctgcag caacgcgcgc tgagacagcg gcaaaacggg cagaggatat
     9241 tgcatccgcc gtggcgcttg aggatgcgaa cacgacgaaa aaggggatag tccagctcag
     9301 tagtgcaacc aacagcacat ctgaaacact tgccgcgaca ccgaaggcag ttaaagcagc
     9361 gaatgacaat gcgaatggca gggtaccatc taaccgaaaa gttaacggga aagcactgac
     9421 tgcggatata acattaacac cgaaagatat tggcacttta aattcagtaa cgatctcttt
     9481 ctctggcggg gcagggtggt tcaaactggc aacggttacc atgccacaag cgagttccat
     9541 cgtttacatc gcattgattg gtggcgctgg ttacaacgtc gtctccccac agcaggcagg
     9601 catttcagaa ctggttctac gagcaggcaa tggaaacccc aaagggatta ccggagcttt
     9661 gtggaagcgt acagccgtcg gattaacgaa tttcgcctgg atcaacacat ccggcgatac
     9721 atatgatatt tacgttgaga ttggcaatta tgcgacgagt gtaaatatcc attgggattg
     9781 tactacaaat gcgtcagttt ctatttatac ctcgccaaca tattcagcga gtaagccttc
     9841 cagcgttacc gatggtgttg tttatacgat gtatagctca catcagaaac ctacaccatc
     9901 agatattgga gcgctgccaa cgactggagg gactatttca ggtccgttgt ctgttactga
     9961 tgggatcacc ggggcactga aggggaacgc cgataccgcg acgaaacttg cggcagcccc
    10021 aaaaattaac ggtgttaagt ttgatggctc ggcggatatt aacctcacgc cggaaaatat
    10081 tggtgcattt gcccgacgtt cgacgggggc ttatgcggat tcggatggag ccgttccctg
    10141 gaatgccgaa tcaggcgctt acaatgtcac ccgctctggc gacagctata ttctggttaa
    10201 cttctatacc ggagtcggaa gttgccggac cttgcagatg aaggcgcatt acagaaatgg
    10261 tggtctgttc taccgttctt caagagacgg ttatggtttt gaggaagaca gggcagaagt
    10321 ttatacctcg aaaaatcttc caccagaaag ctacccagtc ggcgcaccaa tcccgtggcc
    10381 atcagatacc gttccgtctg gttatgccct gatgcagggg cagacttttg acaaatctgc
    10441 ctacccgaaa cttgcagccg cttatccgtc aggcgtgatc cctgatatgc gtggctggac
    10501 gattaagggc aaacctgcca gtggtcgtgc cgtattgtct caggaacagg acggcattaa
    10561 atcgcacacc cacagcgcca gtgcatccag tacggatttg gggacgaaaa ccacgtcgtc
    10621 gtttgattat ggtactaagt cttcaaatgc cactggtgac cataaccaca gccgaggcac
    10681 tatggaaatt accggtactc tcggttactt cagaagtgat agcggtaatt tctatacagc
    10741 aagtgaagca tttacactgg gtggctctgc gccagcccat ggatttacgg gctctcaatt
    10801 tacttatggt gttcctgtaa actttaacgc ttccagaacc tggtctggtg ccacaagcac
    10861 tacaggtaac catgcgcact ctgttccaat tggtgctcat gcgcactccg ttgcgattgg
    10921 ttcacatgga cacaccatca ccgttaacgc tgctggtaac gcggaaaaca ccgtcaaaaa
    10981 catcgcattt aactatattg tgaggcttgc ataatggcat tcagaatgag tgaacaacca
    11041 cggaccataa aaatttataa tctgctggcc ggaactaatg aatttattgg tgaaggtgac
    11101 gcatatattc cgcctcatac aggtctgcct gcaaacagta ccgatattgc accgttagat
    11161 attccggcag gcttcgtggc tgttttcaac agtgatgagt catcgtggca tctctttgaa
    11221 gaccatcggg gtaaaacggt ttatgacgtg gcttccggcg acgcgttatt tatttctgaa
    11281 ctcggtccgt taccggaaaa tgttacctgg ttatcgccgg aaggggagtt tcagaagtgg
    11341 aacggcacag cctgggtgaa ggatacggaa gcagaaaaac tgttccggat ccgggatgcg
    11401 gaagaaacaa aaaacaacct gatgcaggta gccagtgagc atattgcgcc gcttcaggat
    11461 gctgcagatc tggaaattgc aacggaggaa gaaacctcat tgctggaagc ctggaaaaag
    11521 tatcgggtgt tgctgaaccg tgttgataca tcaactgcac ctgatattga gtggcctacg
    11581 aaccctgtca gggagtaatc attgggatta tgccgcagca cgtcttaagc aagaacgtgc
    11641 tgcggttgga tggtgaactt tcgatagtgc gagtattgaa tgatttccag ccgttaccga
    11701 ttttacgtgt taattagtga acaaaccacg cgtcagcaga ctcccaggta tctttcagag
    11761 tttcctgaac aaaagtttta gctgaatctt tatcggcggt gcgcgtaaca gaaaggccat
    11821 cgttgctggt agcttttacg atcacctcta catcgtcata acgtttactg atgcgtcggg
    11881 ttaattcttc ctttaacgca tccacagcac cggttggcat tttagtcatt ttttctttgg
    11941 ctatgcagat ttcaatacgc ataaaagtcc ctctatactg tgtttgtata cagtattatt
    12001 tttaactgta tggataaaca gtgtcaagag atcttatttc tgctcctttg gagctcttca
    12061 aaacgattat gtaaagattt cggatacagt tcggtatata cctgccatag cacgtttaat
    12121 gaacgatgcc ctgtaacctg ggctacttcc tcaatactaa aaccagcctc aaataagcga
    12181 cttgcccctt ctctacgcaa atcatggtat cgcagatcct taatacctaa tttgcttctt
    12241 accctctgaa atcccgcagt aacagaagtg ctgttatatg gaaaaatgaa ttccgatttt
    12301 tggggctgtc gttggacgat atcccaggct tccccaagca aggctacttt catgtggttg
    12361 ccttcctttt tgcgtggatc tttcctgtct cttacgagta tagatttttg ttcctggtcg
    12421 agatcttctc atcgtaaccg gcatacttct ccgattcgca tacaggacca cacagaaaat
    12481 ttgaggatat caacgaacgg aatttttgag catttatgag tagatcgttg ttgaaggcct
    12541 tcaatgagca tgtccagttc atcagatgct ggtctacgat tacgacggtt tgatttacca
    12601 atcaaaccaa gtttaagtag gtatggacga gcacttttcg ccgggtttga tgtgtaatta
    12661 attccgtata caggtttggc cgcatccaga acactgccaa gataactaac atcgtggctg
    12721 actgttgctg gacttgcgcc agcgttgttt cttagcctgc aatgttcaat tacgtcattt
    12781 tctgtcagtt cagatagttt gatcgcggag atgtcactat ccataagcag ttccagcaca
    12841 tatcttttag tacggcctgc tttacctccg gcatttgggt catttaaata tttgtgtagt
    12901 aagtcacgga ctgtaagtcc gtcaactgca tttgatgatg gaattccata tagatctaat
    12961 tccatcactt tctgtgtgcc ccatgttttg gcatgagcat gtttagggaa tgttttgctt
    13021 tccctgtaag tgataacacc tttttctttg ataatcacat tacagcgata gcgtggtgtg
    13081 ccatcggatt ttagtcgttt ctctatgtta tagtacgcca ttacacgacc tcgttatttc
    13141 gggttcccat aaaacgtggg aacctgtgcg ggaacctgac gcgagaaaaa tagcctgaaa
    13201 tgttcaaaaa tgcacgataa tcatgaaaca caaaaaatta atcaaaccag cgtgatgcct
    13261 gaaaaaactg gtgtttactg gaattctcgg tttagcgttg caccaatgct cgactggacg
    13321 gacagacatt gccgctattt cttgcgtctg ctttcccgca atacgttgct ttataccgaa
    13381 atggtgacca caggggcgat tattcacggt aaaggtgatt atctggcgta cagtgaagaa
    13441 gaacatccgg tagcgttgca actgggcggt agcgatccgg cggcgctggc gcagtgtgca
    13501 aagctggcag aagcgcgcgg atatgatgag atcaacctga atgtcggctg cccgtctgac
    13561 cgggtgcaga acggcatgtt tggtgcgtgt ctgatgggta atgcgcagct ggttgccgac
    13621 tgcgtgaaag cgatgcgcga tgtggtgtcg attccggtga cggtgaaaac gcgtattggc
    13681 atcgacgacc aggacagcta tgaatttctc tgcgatttca tcaataccgt ttccggcaaa
    13741 ggcgagtgtg agatgttcat catccacgca cgtaaagcct ggctttcggg gttaagcccg
    13801 aaagaaaacc gtgaaatccc gccgctcgat tatccgcgtg tgtatcaact gaagcgtgac
    13861 tttccgcatc tgacaatgtc gattaacggt ggtatcaagt cgctggaaga agctaaagcg
    13921 catttgcaac atatggatgg cgtgatggtc gggcgcgagg cgtatcagaa tccgggtatt
    13981 ctggcggcgg tagaccggga gatctttggt tcctcggata ccgatgccga tccggtggca
    14041 gtagtgcgtg ccatgtatcc gtacattgag catgaactca gccaggggac gtatctcggc
    14101 catattatcc ggcatatgct gggcttgttc ctgggtattc ctggcgcgcg gcagtggcgg
    14161 cgttatttaa gtgaaaatgc ccataaagcg ggtgctgaca ttaacgtgct ggaacacgcg
    14221 ctcaaactgg tggcggataa gcgttaactt ttcaccaaaa agtagtcaaa ttcaccacgc
    14281 cctgcgcacc gtcgcggggc gttttgctgt taaatcaata gattattttt ggcatgattc
    14341 ttgtaatgcc agcaagagat ttcatatttg ggagagcatc atgctggaac tactttttgt
    14401 gattggcttt tttgtcatgc tgatggtcac cggcgtttcg ttgctgggca ttatcgccgc
    14461 gctggttgtg gcgacggcca ttatgttcct cggcggtatg ctggcattga tgattaagtt
    14521 gctgccgtgg ttactactgg cgattgcggt ggtgtgggtt attaaggcga ttaaagcacc
    14581 aaaaatgccg aaatatcagc gttatgaccg ctggcgttac taagggattg tgcggatgat
    14641 cacaacctaa ggttttatcc ttagaacaaa ataggaattg ataatcaaat ctgtcactat
    14701 tgcgcctcta acagattcat cgtgctgtac cctacataca gccgaactat aaaaagaaag
    14761 ggcttcccag gtggaagccc tatttctttt atggaatcag caggctggaa ccttgcgtcg
    14821 cccggctttc cagaatctca tgcgcacgct gcgcatcctt cagcggatat ttctgctgct
    14881 cggcgacatc gaccttaatc acaccgctgg caatcaaaga gaacagttca ttactggcct
    14941 cggttaattc ctcccgcgtg gtgatatagc cttgcaggga agggcgtgtc acatacaacg
    15001 agcctttttg attgagaatg cctaagttca caccggtaac cgcacctgat gagttgccaa
    15061 aactgaccat taagccgcgg cgttgcaggc aatccagcga ccgttcccag gtgtctctgc
    15121 ccacggaatc gtacactacg cgcactttct taccgccggt gatctctttc aatcgctcga
    15181 ccagattctc ttcacgatag ttaataacct gccacgcgcc ggcttttagc gcgctctgcg
    15241 ctttttgcgc ggttcctacg gtgccgatga gtttcgcgcc cagggctttt gcccactggc
    15301 aggcaattaa gccaacgccg ccagccgctg cgtggaacag gaactgttca tcgggtttaa
    15361 tttcataggt tttgcgcagc agataataaa ccgttaagcc tttcaggaag gatgccgcag
    15421 cttgctcaaa agaaattgcc gcaggcagaa tcgccgcttt atccgcatta atgttatgca
    15481 cagagctgta agcgcctaac gccgactgcg catagactac atgatcgcca gccttaatat
    15541 gctttacacc actgccgact ttactcacga tgcctgccgc ttcggtacct aatccgctgg
    15601 gtagcgatgg cggcgggtaa aggccgctgc ggatatacgt gtcgataaaa ttgatgccga
    15661 tggctttatt ttcgacctgg atttcattct ccgccggatc ggcaggagtg aactctacgg
    15721 cttgaagtac ttccgggcca ccgtgcttgt gaaattcaat tcgtgttgcc atgtgtcctc
    15781 cagaacgtat cgtcagggtc tgcttcatat ggtaaagttt cgacccattc tttatctcgg
    15841 taactccatt cactatggca ggaaataaac ccttcaacaa acagcaggct gaaccccgcg
    15901 aacgcgatcc acaagttgcc gggctgaaag tgcctccgca ctcgatcgaa gcggagcagt
    15961 cggtgttggg cggtttaatg ctggataacg aacgctggga tgatgtagcc gagcgtgtgg
    16021 tagcagacga tttttacacc cgcccacacc gtcatatctt tactgaaatg gcgcgtttgc
    16081 aggaaagcgg tagtcctatc gatctgatta cccttgcgga atcgttggaa cgccaggggc
    16141 aactcgatag cgtcggtggt tttgcttatc tggcagagct gtcaaaaaat acgccaagtg
    16201 cggcgaacat cagtgcttat gcggacatcg tgcgtgaacg tgccgttgtc cgtgagatga
    16261 tctcggttgc gaatgagatt gctgaagccg gttttgatcc gcaggggcgt accagcgaag
    16321 atctgctgga ccttgctgaa tcccgcgtct ttaaaattgc cgaaagtcgt gcaaacaaag
    16381 acgaagggcc gaagaacatc gccgatgtgc tcgacgcaac cgtggcgcgt attgagcagt
    16441 tgtttcagca gccacacgat ggcgttaccg gggtaaacac cggttatgac gatctcaaca
    16501 aaaaaaccgc tggcttgcag ccgtcggatt tgatcatcgt cgccgcgcgt ccgtcgatgg
    16561 gtaaaacaac atttgcgatg aacctcgtcg aaaacgcggc aatgttgcag gataaaccag
    16621 tacttatctt ctcgctggag atgccatcag aacagatcat gatgcgttct ctggcgtcgc
    16681 tgtcgcgcgt tgaccagact aaaatccgta ccgggcagct cgatgatgaa gactgggcgc
    16741 gcatttccgg caccatgggt attttgctcg aaaaacgcaa tatctatatc gatgactctt
    16801 ccggcctgac gccaacggaa gtgcgttccc gcgcacgccg tattgcccgt gaacacggcg
    16861 gcatcgggct tatcatgatc gactacctgc aactgatgcg cgtaccggcg ctttccgata
    16921 accgtacgct ggaaattgca gaaatctccc gctcgctgaa agcactggcg aaagaactga
    16981 acgtgccggt ggtggcgctg tcccagttga accgttctct ggaacaacgt gccgacaaac
    17041 gcccggtcaa ctccgacctg cgtgaatctg gctctatcga gcaggatgcg gacttgatca
    17101 tgtttatcta tcgtgatgag gtgtatcacg aaaacagtga tttaaaaggc atcgcggaaa
    17161 ttattatcgg taaacaacgt aacggcccaa tcgggacggt acgcctgacc tttaacggtc
    17221 aatggtcgcg cttcgacaac tatgcggggc cgcagtacga cgacgaataa taattatttt
    17281 atgaattagg taattaaagc aaacacttat caaggaacac aaatgcaagc ggcaactgtt
    17341 gtgattaacc gccgcgctct gcgacacaac ctgcaacgtc ttcgtgaact ggcacctgcc
    17401 agtaaaatgg ttgcggtggt gaaagcgaac gcttatggtc acggtcttct tgagaccgcg
    17461 cgaacgctcc ccgatgctga cgcctttggc gtagcccgtc tcgaagaagc tctgcgactg
    17521 cgtgcggggg gaatcaccaa acctgtactg ttactcgaag gcttttttga tgccagagat
    17581 ctgccgacga tttctgcgca acattttcat accgccgtgc ataacgaaga acagctggct
    17641 gcgctggaag aggctagcct ggacgagccg gttaccgtct ggatgaaact cgataccggt
    17701 atgcaccgtc tgggcgtaag gccggaacag gctgaggcgt tttatcatcg cctgacccag
    17761 tgcaaaaacg ttcgtcagcc ggtgaatatc gtcagccatt ttgcgcgcgc ggatgaacca
    17821 aaatgtggcg caaccgagaa acaactcgct atctttaata ccttttgcga aggcaaacct
    17881 ggtcaacgtt ccattgccgc gtcgggtggc attctgctgt ggccacagtc gcattttgac
    17941 tgggtgcgcc cgggcatcat tctttatggc gtctcgccgc tggaagatcg ctccaccggt
    18001 gccgattttg gctgtcagcc agtgatgtca ctaacctcca gcctgattgc cgtgcgtgag
    18061 cataaagccg gagagcctgt tggttatggt ggaacctggg taagcgaacg tgatacccgt
    18121 cttggcgtag tcgcgatggg ctatggcgat ggttatccgc gcgccgcgcc gtccggtacg
    18181 ccagtgctgg tgaacggtcg cgaagtaccg attgtcgggc gcgtggcgat ggatatgatc
    18241 tgcgtagact taggtccaca ggcgcaggac aaagccgggg atccggtcat tttatggggc
    18301 gaaggtttgc ccgtagaacg tatcgctgaa atgacgaaag taagcgctta cgaacttatt
    18361 acgcgcctga cttcaagggg cgcgatgaaa tacgtggatt aatcgttctg taatatttga
    18421 ttgtctgtgc cggatgcggc gtgaatgcct tatccggcca ataaaatcct aaaaattcaa
    18481 taagttgatg ttctttcatg ctcttataaa ggtcgtgcct ctggcggatg tacgtttatc
    18541 attagtctca ctctgttgct aattgccgtt cgctcctgaa catccactcg atcttcgcct
    18601 tcttccggtt tattgtgttt taaccacctg cccgtaaacc tggagaacca tcgcgtgttt
    18661 caaaaagttg acgcctacgc tggcgacccg attcttacgc ttatggagcg ttttaaagaa
    18721 gaccctcgca gcgacaaagt gaatttaagt atcggtctgt actacaacga agacggaatt
    18781 attccacaat tgaaagccgt ggcggaggcg gaagcgcgcc tgaatgcgca gccccatggc
    18841 gcttcgcttt atttaccgat ggaagggctt aacagctatc gccatgccat tgcgccgctg
    18901 ctgtttggtg cggaccatcc ggtactgcaa caacagcgcg tagcaaccat tcaaaccctt
    18961 ggcggctcag gggcattgaa agtgggcgcg gatttcctga aacgctactt cccggaatca
    19021 ggcgtctggg tcagcgatcc tacttgggaa aaccacgtag caatattcgc cggggctgga
    19081 ttcgaagtga gtacttaccc ctggtatgac gaagtgacta acagcgtgcg ctttaatgac
    19141 ctgttggcga cgctgaaaac attacctgcc cgcagtattg tgttgctgca tccatgttgc
    19201 cacaacccaa cgggtgccga tctcactaat gatcagtggg atgcggtgat tgaaattctc
    19261 aaagcccgcg agcttatccc attcctcgat attgcctatc aaggatttag tgccggtatg
    19321 gaagaggatg cctacgctat tcgcgccatt gccagcgctg gattacccgc tctggtgagc
    19381 aattcgttct cgaaaatttt ctccctttac ggcgagcgcg tcggcggact ttctgttctg
    19441 tgtgaagatg ccgaagccgc tggccgcgta ctggggcaat tgaaagcaac agttcgccgc
    19501 aactactcca gcccgccgaa ttttggtgcg caggtggtgg ctgcagtgct gaatgacgag
    19561 gcattgaaag ccagctggct ggcggaagta gaagagatgc gtactcgcat tctggcaatg
    19621 cgtcaggaat tggtgaaggt attaagcaca gagatgccag aacgcaattt cgattatctg
    19681 cttaatcagc gcggcatgtt cagttatacc ggtttaagtg ccgctcaggt tgaccgacta
    19741 cgtgaagaat ttggtgtcta tctcatcgcc agcggtcgca tgtgtgtcgc cgggttaaat
    19801 acggcaaatg tacaacgtgt ggcaaaggcg tttgctgcgg tgatgtaatg caggaaagca
    19861 ggctggagtt acccagcctg cagtgaaatt aaactgtcgt cgctttcact atttctttgt
    19921 agatgatttt tttgatgccg ttgttctacg tgagagataa taaaacgttg tcagttcttt
    19981 tattgttaag tttatcccaa ttatctggaa ttcctttccc tattttttcg tgtggagtaa
    20041 taatttctgc acatctaacc attattaaat ttaataataa gtatcgggtt tgttacgata
    20101 gaatatttct atatcctcaa gaatatgctt tgccttattg cctgtgactt tctgagattc
    20161 aattatagta tcgttaaatt ctaatgttaa agagaactct ttttttccgc tttgtgtaag
    20221 tttaacctta tattgattac caatatcaaa taaaaaggtc ctgtaatgag aataatcacc
    20281 tttaacctcc tcggcaaaac agcaaagcgt gcaaatatac ataaagtctt tcttacccct
    20341 gattttgtta actaaatcaa taaatgcgtg aaaaacttta cttgcaatag aacttgatac
    20401 ttcttctcta ggtctacaaa aatcttttgc aatttttaac tgtgtttcta tgtataaaaa
    20461 gaaatctttg gcataagaga aatctttggc ataagagata tctttattat ttagtgcatc
    20521 tagctcaaca ggtaaagatg tcgttttgtt gctattcaca tatgatatat tcatatcatc
    20581 atctccatcg cgagtaataa aattaatcac cattgtaggg tagggggctg gtcaatcaga
    20641 aatcatcttt ataaacttcg attgtttttg taatgctgta tcattaagtt cattaaatcg
    20701 tacagcagat aaatgttcta tcaaatttcg ctcatttgcc gaggattcat cataataaac
    20761 gtaaaattaa tgtatcctta catcgagtaa taaacatttt ttatacaaaa aaagacagga
    20821 acgtatttac tgggttaaat ataatcatcc tgcttttcat cacaaaaacc gcagataatc
    20881 cttcctttcc ccggcagctg gcgttatggt cagatggttt ttgcaacaaa tctcacaata
    20941 aaaagtttca acatactgac tatttaggga aaaatatgcg caagatcaca caggcaatca
    21001 gtgccgtttg cttattgttc gctctaaaca gttccgctgt tgccctggcc tcatctcctt
    21061 caccgcttaa ccctgggact aacgttgcca ggcttgctga acaggcacca attcattggg
    21121 tttcggtcgc acaaattgaa aatagcctcg cagggcgtcc gccaatggcg gtggggtttg
    21181 atatcgatga cacggtactt ttttccagtc cggggttctg gcgcggcaaa aaaaccttct
    21241 cgccagaaag cgaagattat ctgaaaaatc ctgtgttctg ggaaaaaatg aataatggct
    21301 gggatgaatt cagcattcca aaagaggtcg ctcgccagct gattgatatg catgtacgcc
    21361 gcggtgacgc gatcttcttt gtgactggtc gtagcccgac gaaaacagaa acggtttcaa
    21421 aaacgctggc ggataatttt catattcctg ccaccaacat gaatccggta atctttgcgg
    21481 gcgataaacc agggcaaaat acaaaatcgc aatggctgca ggataaaaat atccgaattt
    21541 tttatggcga ttccgataac gatattaccg ccgcacgcga tgtcggcgct cgtggtatcc
    21601 gcattctgcg cgcctccaac tctacctaca aacccttgcc acaagcgggt gcgtttggtg
    21661 aagaggtgat cgtcaattca gaatactgac agagcgggag agagtgatgc tctcccgaat
    21721 gctgtttttt taatcacacc tttatccttt cgctgtcttg ctgcaaactg attaagagag
    21781 ttttatcaag gagcagcaca tgtggtatca aaagacgctc acgcttagcg ccaaatctcg
    21841 tgggtttcat ctggtaacgg atgaaattct gaatcagctg gctgatatgc cgcgcgttaa
    21901 catcggctta ctgcatctgt tgctgcaaca tacctccgcc tctctgacac ttaatgagaa
    21961 ctgcgatccc accgtacgcc acgacatgga gtgttttttc ctccgcaccg ttcccgacaa
    22021 cggaaattat gagcatgact atgagggagc agacgatatg ccttctcata tcaaatcctc
    22081 aatgctggga acatcgcttg tattgccggt acataaaggg cgtattcaga ccggcacttg
    22141 gcaaggcatc tggctgggag aacatcgcat ccacggcgga tcgcgtcgca tcatcgcgac
    22201 actacaaggg gagtaaaaaa tgaccatttc ggagttgcta caatattgca tggcaaaacc
    22261 aggcgcagaa cagagcgtgc ataacgactg gaaagcgacg cagatcaaag tggaagatgt
    22321 actgtttgcg atggtgaaag aagtagaaaa tcgcccagct gtttcgctga aaaccagccc
    22381 ggagctggcg gagctgctac gtcagcagca cagcgatgtg cgtcctagcc gccatctgaa
    22441 taaagcgcac tggagcaccg tgtatctcga cggttcgctg ccggattcgc aaatctatta
    22501 tctggtggat gcgtcttatc agcaggcagt gaatttacta ccggaagaaa agcgtaaatt
    22561 gctggtacaa ctctgaaagg aaaaggccgc tcagaaagcg gccttaacga ttacagcatc
    22621 ggcttgagga agcgtgccgt atgcgaagct tcgcactccg cgacggtttc tggcgtaccg
    22681 gagacgagga tttcgccgcc gccactgccg ccttctggtc ccaggtcgac aatccagtca
    22741 gcggttttga tcacgtcgag attgtgctca atcaccacaa tggtattgcc ctgatcgcgc
    22801 agtttatgca gcacgtcgag gagttgctga atatcggcga agtgcagacc gctggtcggc
    22861 tcgtcgagaa tatacagcgt ctgcccggtg ccgcgttttg acagctcacg cgccagcttc
    22921 acgcgctggg cttcaccacc agaaagtgtg gttgcggact gccccaggcg aatgtacgtc
    22981 agaccaacgt ccatcaacgt ttgcagctta cgcgccaggg ctggcaccgc atcaaagaac
    23041 tcacgcgcct cttcgatggt catatccagc acttcgtgga tggttttgcc tttgtactta
    23101 atttccagcg tttcacggtt atagcgttta cctttgcact gatcgcacgg cacgtagata
    23161 tccggcagga agtgcatctc cactttgatc acaccgtcgc cctgacaggc ttcgcagcgc
    23221 ccgccacgga cgttaaagct gaaacgtcct ggcgtataac cacgcgcacg ggattccggt
    23281 acgcccgcaa acagttcgcg cacaggcgta aacacgccgg tataggtcgc cgggttagaa
    23341 cgtggagtac gaccaattgg gctttggtcg atatcgatca ctttatcgaa atgctccagt
    23401 ccctgaatat cgcgatacgg tgccggttcg gcgatggtcg caccattcaa ctggcgttgg
    23461 gcaatcggga acagtgtgtc gttaatcagc gtcgatttac cggaacctga aacccctgtg
    23521 atgcaggtaa acagaccgac tggcagcgtg agcgtcacgt ccttcaggtt gttgccgcgt
    23581 gcgcctgtca gcttcagcac tttttctgga ttcgccggaa cgcgtttctt cggcacttca
    23641 atcttgcgtt taccgctcat gtactgcccg gtcaacgatt caggcaccgc cataatcgct
    23701 tccagcggac cttctgcgac cacttcaccg ccgtgaacac ccgcaccagg accgatatcg
    23761 atcacatggt cagcggcgcg aattgcgtct tcgtcatgct ccaccacaat cacggtatta
    23821 ccgagatcgc gcagatggat aagcgtaccc aacaggcgct cgttatcgcg ctggtgcagg
    23881 ccgatagacg gctcgtccag cacgtacata acgccaacca ggcccgcacc aatctggctc
    23941 gccagacgga tacgctgggc ttcaccgccg gaaagtgttt ctgccgagcg ggaaagtgtc
    24001 aggtaattca ggccgacgtt tacgaggaat ttcaggcgat cgccgatctc tttaagaatt
    24061 ttttccgcaa tcttcgcccg ctgaccagcg agtttgagat tgttgaagaa ttccatcgca
    24121 tgaccgatgc tcatgtcgga gatagcaggc agcggcgtat tctcgacata aacgtggcgc
    24181 gcttcccgac gcagacgcgt cccttcgcag ctggcgcatg ggcgattgct gataaacttg
    24241 gctaattctt cacgtaccgc actggattct gtctctttat aacggcgctc catattgtgc
    24301 agcacgcctt cgaacggatg acgacggatg gaggtatcgc cacgatcgtt catgtatttg
    24361 aattcaatgt tttctttgcc agaaccgtac aacaccactt tatgcacgtt cgcgctcagg
    24421 ctgccccacg gcgcttcgac gtcgaactta tagtgatctg ccagcgattt cagcatctgg
    24481 aagtaataga agttgcggcg atcccagcca cggatcgcac cgccagccag tgacagctcg
    24541 gggttttgga tcacgcggtc aggatcgaaa tattgctgta cgccaaggcc gtcacaggtc
    24601 gggcaggcac ctgccgggtt gttaaacgaa aacagtcgcg gctccagttc acgcatactg
    24661 tagccgcaaa ttgggcaggc gaagttggcg gagaacagca gctcttccgc tttcgggtcg
    24721 tccatatccg ccactaccgc tgtaccaccg gaaagttcca gcgcggtttc aaacgactcc
    24781 gcaagacgtt gggtaagatc gtcacgcacc ttgaagcgat caaccaccac ttcaatggta
    24841 tgtttctttt gcagttccag tttcggcgga tcggaaagat cgcagacttc gccatcaata
    24901 cgagcacgga tgtaaccctg gcttgccagg ttctccagcg ttttggtgtg ttcgcctttg
    24961 cgctctttaa tgattggcgc aagcagcatc agacgcttgc cttccggctg cgacagcacg
    25021 ttatccacca tctggctgac ggtttgcgcc gccagcggga cgtcgtggtc cggacagcgc
    25081 ggttcgccga cgcgggcgaa caacaaacgc aaatagtcgt ggatttcggt gattgtcccc
    25141 accgtagaac gcgggttatg agacgtcgat ttctgctcaa ttgagatggc aggagaaagc
    25201 ccctcaatat gatcgacgtc cggcttttcc atcagtgaca gaaactgccg cgcgtaggcg
    25261 gaaagggatt caacgtaacg gcgctgccct tcggcatata aggtgtcgaa agcgagcgag
    25321 gatttgccag aacccgaaag cccggtcacg acaatgagct tgtcgcgggg aataacgagg
    25381 ttgatgtttt tgagattatg ggtgcgggcg ccccgaactt cgatcttatc cattcacctt
    25441 tcccggatta aacgcttttt tgcccggtgg catggtgcta ccggcgatca caaacggtta
    25501 attatgacac aaattgacct gaatgaatat acagtattgg aatgcattat ccggagtgtt
    25561 gtgtaacaat gtctggccag gtttgtttcc cggaaccgag gtcacaacat agtaaaagcg
    25621 ctattggtaa tggtacaatc gcgcgtttac acttattcag aacgattttt ttcaggagac
    25681 acgaacatgg ccagcagagg cgtaaacaag gttattctcg ttggtaatct gggtcaggac
    25741 ccggaagtac gctacatgcc aaatggtggc gcagttgcca acattacgct ggctacttcc
    25801 gaatcctggc gtgataaagc gaccggcgag atgaaagaac agactgaatg gcaccgcgtt
    25861 gtgctgttcg gcaaactggc agaagtggcg agcgaatatc tgcgtaaagg ttctcaggtt
    25921 tatatcgaag gtcagctgcg tacccgtaaa tggaccgatc aatccggtca ggatcgctac
    25981 accacagaag tcgtggtgaa cgttggcggc accatgcaga tgctgggtgg tcgtcagggt
    26041 ggtggcgctc cggcaggtgg caatatcggt ggtggtcagc cgcagggcgg ttggggtcag
    26101 cctcagcagc cgcagggtgg caatcagttc agcggcggcg cgcagtctcg cccgcagcag
    26161 tccgctccgg cagcgccgtc taacgagccg ccgatggact ttgatgatga cattccgttc
    26221 tgatttgtca ttaaaacaat aggttatatt gttttaaggt ggatgattaa agcatctgcc
    26281 agccataaaa aagaagcctc cgttatggag g
//
LOCUS       DADLSA010000044        26178 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.044, whole genome shotgun sequence.
ACCESSION   DADLSA010000044 DADLSA010000000
VERSION     DADLSA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.044"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            240..434
                     /gene="bfd"
                     /locus_tag="JN691_001835"
     CDS             240..434
                     /gene="bfd"
                     /locus_tag="JN691_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAY7243293.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            507..983
                     /gene="bfr"
                     /locus_tag="JN691_001836"
     CDS             507..983
                     /gene="bfr"
                     /locus_tag="JN691_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAY7243294.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(980..1447)
                     /locus_tag="JN691_001837"
     CDS             complement(980..1447)
                     /locus_tag="JN691_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAY7243295.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1826..2137
                     /gene="rpsJ"
                     /locus_tag="JN691_001838"
     CDS             1826..2137
                     /gene="rpsJ"
                     /locus_tag="JN691_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAY7243296.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2170..2799
                     /gene="rplC"
                     /locus_tag="JN691_001839"
     CDS             2170..2799
                     /gene="rplC"
                     /locus_tag="JN691_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAY7243297.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2810..3415
                     /gene="rplD"
                     /locus_tag="JN691_001840"
     CDS             2810..3415
                     /gene="rplD"
                     /locus_tag="JN691_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAY7243298.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3412..3714
                     /gene="rplW"
                     /locus_tag="JN691_001841"
     CDS             3412..3714
                     /gene="rplW"
                     /locus_tag="JN691_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAY7243299.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3732..4553
                     /gene="rplB"
                     /locus_tag="JN691_001842"
     CDS             3732..4553
                     /gene="rplB"
                     /locus_tag="JN691_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAY7243300.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKLGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4570..4848
                     /gene="rpsS"
                     /locus_tag="JN691_001843"
     CDS             4570..4848
                     /gene="rpsS"
                     /locus_tag="JN691_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAY7243301.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4863..5195
                     /gene="rplV"
                     /locus_tag="JN691_001844"
     CDS             4863..5195
                     /gene="rplV"
                     /locus_tag="JN691_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAY7243302.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5213..5914
                     /gene="rpsC"
                     /locus_tag="JN691_001845"
     CDS             5213..5914
                     /gene="rpsC"
                     /locus_tag="JN691_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAY7243303.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5927..6337
                     /gene="rplP"
                     /locus_tag="JN691_001846"
     CDS             5927..6337
                     /gene="rplP"
                     /locus_tag="JN691_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAY7243304.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6337..6528
                     /gene="rpmC"
                     /locus_tag="JN691_001847"
     CDS             6337..6528
                     /gene="rpmC"
                     /locus_tag="JN691_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAY7243305.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6528..6782
                     /gene="rpsQ"
                     /locus_tag="JN691_001848"
     CDS             6528..6782
                     /gene="rpsQ"
                     /locus_tag="JN691_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAY7243306.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6947..7318
                     /gene="rplN"
                     /locus_tag="JN691_001849"
     CDS             6947..7318
                     /gene="rplN"
                     /locus_tag="JN691_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAY7243307.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7329..7643
                     /gene="rplX"
                     /locus_tag="JN691_001850"
     CDS             7329..7643
                     /gene="rplX"
                     /locus_tag="JN691_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAY7243308.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7658..8197
                     /gene="rplE"
                     /locus_tag="JN691_001851"
     CDS             7658..8197
                     /gene="rplE"
                     /locus_tag="JN691_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAY7243309.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8212..8517
                     /gene="rpsN"
                     /locus_tag="JN691_001852"
     CDS             8212..8517
                     /gene="rpsN"
                     /locus_tag="JN691_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAY7243310.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8551..8943
                     /gene="rpsH"
                     /locus_tag="JN691_001853"
     CDS             8551..8943
                     /gene="rpsH"
                     /locus_tag="JN691_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAY7243311.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8956..9489
                     /gene="rplF"
                     /locus_tag="JN691_001854"
     CDS             8956..9489
                     /gene="rplF"
                     /locus_tag="JN691_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAY7243312.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9499..9852
                     /gene="rplR"
                     /locus_tag="JN691_001855"
     CDS             9499..9852
                     /gene="rplR"
                     /locus_tag="JN691_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAY7243313.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9867..10370
                     /gene="rpsE"
                     /locus_tag="JN691_001856"
     CDS             9867..10370
                     /gene="rpsE"
                     /locus_tag="JN691_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAY7243314.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10374..10553
                     /gene="rpmD"
                     /locus_tag="JN691_001857"
     CDS             10374..10553
                     /gene="rpmD"
                     /locus_tag="JN691_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAY7243315.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10557..10991
                     /gene="rplO"
                     /locus_tag="JN691_001858"
     CDS             10557..10991
                     /gene="rplO"
                     /locus_tag="JN691_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAY7243316.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10999..12330
                     /gene="secY"
                     /locus_tag="JN691_001859"
     CDS             10999..12330
                     /gene="secY"
                     /locus_tag="JN691_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAY7243317.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12362..12478
                     /gene="rpmJ"
                     /locus_tag="JN691_001860"
     CDS             12362..12478
                     /gene="rpmJ"
                     /locus_tag="JN691_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAY7243318.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12625..12981
                     /gene="rpsM"
                     /locus_tag="JN691_001861"
     CDS             12625..12981
                     /gene="rpsM"
                     /locus_tag="JN691_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAY7243319.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12998..13387
                     /gene="rpsK"
                     /locus_tag="JN691_001862"
     CDS             12998..13387
                     /gene="rpsK"
                     /locus_tag="JN691_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAY7243320.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13421..14041
                     /gene="rpsD"
                     /locus_tag="JN691_001863"
     CDS             13421..14041
                     /gene="rpsD"
                     /locus_tag="JN691_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAY7243321.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14067..15056
                     /gene="rpoA"
                     /locus_tag="JN691_001864"
     CDS             14067..15056
                     /gene="rpoA"
                     /locus_tag="JN691_001864"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAY7243322.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15097..15480
                     /gene="rplQ"
                     /locus_tag="JN691_001865"
     CDS             15097..15480
                     /gene="rplQ"
                     /locus_tag="JN691_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAY7243323.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15587..15955
                     /locus_tag="JN691_001866"
     CDS             15587..15955
                     /locus_tag="JN691_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HAY7243324.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15966..16391
                     /gene="zntR"
                     /locus_tag="JN691_001867"
     CDS             15966..16391
                     /gene="zntR"
                     /locus_tag="JN691_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HAY7243325.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16447..16665
                     /locus_tag="JN691_001868"
     CDS             16447..16665
                     /locus_tag="JN691_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HAY7243326.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16662..17072)
                     /gene="mscL"
                     /locus_tag="JN691_001869"
     CDS             complement(16662..17072)
                     /gene="mscL"
                     /locus_tag="JN691_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAY7243327.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGGIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(17202..18578)
                     /gene="trkA"
                     /locus_tag="JN691_001870"
     CDS             complement(17202..18578)
                     /gene="trkA"
                     /locus_tag="JN691_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAY7243328.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18600..19889)
                     /gene="rsmB"
                     /locus_tag="JN691_001871"
     CDS             complement(18600..19889)
                     /gene="rsmB"
                     /locus_tag="JN691_001871"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAY7243329.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(19935..20882)
                     /gene="fmt"
                     /locus_tag="JN691_001872"
     CDS             complement(19935..20882)
                     /gene="fmt"
                     /locus_tag="JN691_001872"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAY7243330.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20897..21406)
                     /gene="def"
                     /locus_tag="JN691_001873"
     CDS             complement(20897..21406)
                     /gene="def"
                     /locus_tag="JN691_001873"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAY7243331.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21537..22661
                     /gene="dprA"
                     /locus_tag="JN691_001874"
     CDS             21537..22661
                     /gene="dprA"
                     /locus_tag="JN691_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAY7243332.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGLLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22633..23106
                     /locus_tag="JN691_001875"
     CDS             22633..23106
                     /locus_tag="JN691_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAY7243333.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23135..23677
                     /locus_tag="JN691_001876"
     CDS             23135..23677
                     /locus_tag="JN691_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAY7243334.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23682..24254
                     /gene="tsaC"
                     /locus_tag="JN691_001877"
     CDS             23682..24254
                     /gene="tsaC"
                     /locus_tag="JN691_001877"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAY7243335.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24259..25077
                     /gene="aroE"
                     /locus_tag="JN691_001878"
     CDS             24259..25077
                     /gene="aroE"
                     /locus_tag="JN691_001878"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAY7243336.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEEVAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25074..25331
                     /locus_tag="JN691_001879"
     CDS             25074..25331
                     /locus_tag="JN691_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAY7243337.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25307..25861)
                     /locus_tag="JN691_001880"
     CDS             complement(25307..25861)
                     /locus_tag="JN691_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAY7243338.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6725 a         6202 c         6793 g         6458 t
ORIGIN      
        1 atccgtgaag gcggccgtac cgttggcgcg ggcgttgttg ctaaagttct gggctaatta
       61 cacgttaatt agttttgaat tgaaaagggc gcttcggcgc cctttttgct gcccgtaatt
      121 tgagctcgaa atatttagtc gtaattttgc tcgctgaact atggcgcaaa taaaactgtg
      181 ctattgtaat cataaccatt ctcatttaca ctttgcgcag aatttgaacg ggagcaatca
      241 tgtacgtttg tttgtgtaat ggtgtaagtg acaaaaaaat tcgccaggca gtccgtcagt
      301 ttcatccgca atctttccaa caattgcgta agtttattcc tgtgggaaat caatgcggta
      361 agtgcattcg cgccgcgcgc gaagtgatgc aggacgagtt gatgcagatg ccagaattta
      421 aagagattgc ctgaagccca cgcttatttt ttgacatccc gctagcccca tctacgctct
      481 aatagtggaa gcggagggac tataaaatga aaggtgatgt taaaatcata aattatctca
      541 ataaactatt gggaaatgag cttgtcgcaa tcaatcagta ttttctccat gcccggatgt
      601 ttaaaaactg gggcctgatg cgtctcaatg acgtcgaata ccatgaatcc atcgatgaga
      661 tgaaacacgc tgataaatat atcgagcgta ttttgttttt agaaggaatc cccaacttac
      721 aggatttagg caaactgggg attggtgaag atgtcgagga gatgctgcaa tctgacctgc
      781 gattagaact ggaaggcgcg aaggatctgc gtgaggctat cgcatatgcc gacagcgttc
      841 atgactatgt tagccgcgat atgatgatcg agatccttgc tgatgaagaa ggtcatatcg
      901 actggctcga aactgaactg gatctcatcg gtaaaatcgg cctacaaaat tacctgcaat
      961 cacaaattaa agttaaagat taacggccta tcagcaaact atcccagcct ataacgaaac
     1021 ccgcagccgc cagaaatggc ccaaaaggca gcgggttttt taatgattct tttcctctca
     1081 tgagcagacc aataactacg gctccgcaag cgaatgaggc agcaaggaaa accagtcgtg
     1141 gtaagaaggc ccatgaatgc caggcgccaa gggcggcgag aaacttcaca tcaccatagc
     1201 ctaatccttc tttatgtcgc agtatgcgat acccccagta aatgacagcg aatgtgccgt
     1261 aaccaacaat tgccccccat aacgcatcgg ctaaaccatc aggatgacaa acttgataga
     1321 acaaaagacc tgaccagagt aacgggcacg taaatctgtc aggtaacaag ccatgctttg
     1381 catcccagaa aaaaagtagg gctgataaac aagcgtacaa aattagaaag ggaagggtag
     1441 tgtccataaa acatcctctg taattggagt atcaccatcg aataactccg gatgctcagc
     1501 aacagagaga gcgctgattt gttaacctga ttccggtgaa aaacgctatc ttcacaacct
     1561 tacaaatttc gtggaatgca gctcgcaatc tgcgcgttgc gattaatttc tacccgatgc
     1621 ttgcgtcagg ctcagatttg cgtataatgc gcgggattgt cgtagttgac agcaggttca
     1681 atctgaacct cagttagccg aatttggcta cctaacaatg ctcccaatcg gggagctacg
     1741 taagaacggt tacactctcc catcaatcgt aatgggtctg aggagtaatc attttcgttt
     1801 ataaaataat tggagctctg gtctcatgca gaaccaaaga atccgtatcc gcctgaaagc
     1861 gtttgatcat cgtctgatcg atcaagcaac cgcggaaatc gtcgagactg ccaagcgcac
     1921 tggtgcgcag gtccgtggtc cgatcccgct gccgacacgc aaagagcgct tcactgttct
     1981 gatctccccg cacgtcaaca aagacgcgcg cgatcagtac gaaatccgta ctcacttgcg
     2041 tctggttgac atcgttgagc caaccgagaa aaccgttgat gctctgatgc gtctggatct
     2101 ggctgccggt gtagacgtgc agatcagcct gggttaatca ggtcattgag cgattgagag
     2161 gttgaaacaa tgattggttt agtcggtaaa aaagtgggta tgacccgtat cttcacagaa
     2221 gacggcgttt ctatcccagt aaccgtaatc gaagttgaag caaaccgcgt tactcaggtt
     2281 aaagacctgg ctaacgatgg ctaccgtgct attcaggtga ccaccggtgc taaaaaagct
     2341 aaccgtgtga ccaagcctga agctggccac ttcgctaaag ctggcgtaga agctggccgt
     2401 ggtctgtggg aattccgcct ggctgaaggc gaagagttca ctgtaggtca gagcattagc
     2461 gttgaactgt ttgctgacgt taaaaaagtt gacgtaactg gcacctctaa aggtaaaggt
     2521 ttcgcaggta ccgttaagcg ctggaacttc cgtacccagg acgctactca cggtaactcc
     2581 ttgtctcacc gcgttccggg ttctatcggt cagaaccaga ctccgggcaa agtgttcaaa
     2641 ggcaagaaaa tggcaggtca gatgggtaac gaacgtgtaa ccgttcagag ccttgacgta
     2701 gtacgcgttg acgctgagcg caacctgctg ctggttaaag gtgctgtccc gggtgcaacc
     2761 ggtagcgacc tgatcgttaa accagctgtg aaggcgtaag gagatagcaa tggaattagt
     2821 attgaaagac gcgcagagcg cgctgactgt ttccgaaact accttcggtc gtgatttcaa
     2881 cgaagcgctg gttcaccagg ttgttgttgc ttatgcagct ggtgctcgtc agggtactcg
     2941 tgctcagaag actcgtgctg aagtaactgg ttccggtaaa aaaccgtggc gccagaaagg
     3001 caccggccgt gcgcgttctg gttctatcaa gagcccgatc tggcgttctg gtggcgtgac
     3061 ctttgctgct cgtccgcagg accacagtca aaaagttaac aagaagatgt accgcggcgc
     3121 gctgaaaagc atcctgtccg aactggtacg tcaggatcgt ctgatcgttg tcgagaagtt
     3181 ctctgtagaa gcgccgaaaa ctaagctgct ggcacagaaa ctgaaagaca tggctctgga
     3241 agatgtgctg atcatcaccg gtgagctgga cgaaaacctg ttcctggctg cgcgcaacct
     3301 gcacaaggtt gacgtacgcg atgcaactgg tatcgacccg gttagcctga tcgccttcga
     3361 caaagtcgta atgactgctg atgctgttaa gcaagttgag gagatgctgg catgattcgt
     3421 gaagaacgtc tgctgaaggt gctgcgtgca ccgcacgttt ctgaaaaagc gtctactgcg
     3481 atggaaaaat ccaacaccat cgtactcaaa gttgccaaag acgcgaccaa agcagaaatc
     3541 aaagctgctg tgcagaaact gtttgaagtc gaagtcgaag tcgttaacac cctggtagtt
     3601 aaagggaaag ttaaacgtca cggacagcgt atcggtcgtc gtagcgactg gaaaaaagct
     3661 tacgtcaccc tgaaagaagg ccagaatctg gacttcgttg gcggcgctga gtaagtcgga
     3721 ggagtaatac aatggcagtt gttaaatgta aaccgacatc tccgggtcgt cgccacgtag
     3781 ttaaagtggt taaccctgag ctgcacaagg gcaaaccttt tgctccgttg ctggaaaaaa
     3841 acagcaaatc cggtggtcgt aacaacaatg gccgtatcac cactcgtcat atcggtggtg
     3901 gccacaagca ggcttaccgt attgttgact tcaaacgcaa caaagacggt atcccggcag
     3961 ttgttgaacg tcttgagtac gatccgaacc gttccgcgaa catcgcgctg gttctgtaca
     4021 aagacggtga acgccgttac atcctggccc ctaaaggcct gaaagctggc gaccagattc
     4081 agtctggcgt tgatgctgca atcaaactag gtaacaccct gccgatgcgc aacatcccgg
     4141 ttggttctac tgttcataac gtagaaatga aaccaggtaa aggcggtcag ctggcacgtt
     4201 ccgctggtac ttacgttcag atcgttgctc gtgatggtgc ttatgtcacc ctgcgtctgc
     4261 gttctggtga aatgcgtaaa gtagaagcag actgccgtgc aactctgggc gaagttggca
     4321 atgctgagca tatgctgcgc gttctgggta aagcaggtgc tgcacgctgg cgtggtgttc
     4381 gtccgaccgt tcgcggtacc gcgatgaacc cggtagacca cccacatggt ggtggtgaag
     4441 gtcgtaactt tggtaagcac ccggtaactc cgtggggcgt tcagaccaaa ggtaagaaga
     4501 cccgcagcaa caagcgtact gataaattca tcgtacgtcg ccgtagcaaa taattttaga
     4561 ggataagcca tgccacgttc tctcaagaaa ggtcctttta ttgacctgca cttgctgaag
     4621 aaggtagaga aagcggtgga aagcggagac aagaagcccc tgcgcacttg gtcccgtcgt
     4681 tcaacgatct ttcctaacat gatcggtttg accatcgctg tccataatgg tcgtcagcac
     4741 gttccggtat ttgtaaccga cgaaatggtc ggtcacaaac tgggtgaatt cgcaccgact
     4801 cgtacttatc gcggccacgc tgctgataaa aaagcgaaga agaaataagg taggaggaag
     4861 agatggaaac tatcgctaaa catcgccatg ctcgttcttc tgctcagaag gttcgccttg
     4921 ttgctgacct gattcgcggt aagaaagtgt cgcaggctct ggatattttg acctacacca
     4981 acaagaaagc ggctgtactg gtcaagaaag ttctggaatc tgccattgct aacgctgaac
     5041 acaacgatgg cgctgacatt gacgatctga aagttacgaa aattttcgta gacgaaggcc
     5101 cgagcatgaa gcgcattatg ccgcgtgcaa aaggtcgtgc agatcgcatc ctgaagcgca
     5161 ccagccacat cactgtggtt gtgtccgatc gctgagactc tggagactag caatgggtca
     5221 gaaagtacat cctaatggta ttcgcctggg tattgtaaaa ccatggaact ctacctggtt
     5281 tgcgaacacc aaagaattcg ctgacaacct ggacagcgat tttaaagtac gtcagtacct
     5341 gactaaggaa ctggctaaag cgtccgtatc tcgtatcgtt atcgagcgtc cggctaagag
     5401 catccgtgta accattcaca ctgctcgccc gggtatcgtt atcggtaaaa aaggtgaaga
     5461 cgtagaaaaa ctgcgtaagg tcgtagcgga catcgctggc gttcctgcac agatcaacat
     5521 cgccgaagtt cgtaagcctg aactggacgc aaaactggtt gctgacagca tcacttctca
     5581 gctggaacgt cgcgttatgt tccgtcgtgc tatgaagcgt gctgtacaga acgcaatgcg
     5641 tctgggcgct aaaggtatta aagttgaagt tagcggccgt ctgggcggcg cggaaatcgc
     5701 acgtaccgaa tggtaccgcg aaggtcgcgt accgctgcac actctgcgtg ctgacatcga
     5761 ctacaacacc tctgaagcgc acaccactta cggtgtaatc ggcgttaaag tgtggatctt
     5821 caaaggcgag atcctgggtg gtatggctgc tgttgaacaa ccggaaaaac cggctgctca
     5881 gcctaaaaag cagcagcgta aaggccgtaa ataaggagcg tcgctgatgt tacaaccaaa
     5941 gcgtacaaaa ttccgtaaaa tgcacaaagg ccgtaaccgc ggtctggcgc agggtacgga
     6001 tgttagcttc ggcagcttcg gtctgaaagc tgttggccgt ggtcgtctga ctgcccgtca
     6061 gatcgaagca gcacgtcgtg ctatgacccg tgcagttaag cgtcaaggta agatctggat
     6121 ccgtgtgttc ccggacaaac cgatcactga aaagccgctg gcagtgcgta tgggtaaagg
     6181 taaaggtaac gtggagtatt gggttgcctt gattcagccg ggtaaagtcc tgtatgaaat
     6241 ggacggtgtt ccggaagagc tggcccgtga agcattcaag ctggcagcag cgaaactgcc
     6301 gattaaaacc acctttgtaa ctaagacggt gatgtaatga aagcaaaaga gctgcgtgag
     6361 aagagcattg aagagctgaa caccgagctg ctgaacctgc tgcgtgagca gttcaacctg
     6421 cgtatgcagg ctgcaagtgg ccagctgcaa cagtctcacc tgttgaagca agtgcgtcgc
     6481 gatgtcgcac gcgttaagac tttactgaac gagaaggcgg gtgcgtaatg accgataaaa
     6541 tccgtactct gcaaggtcgc gttgttagcg acaaaatgga gaaatccatt gttgttgcta
     6601 tcgaacgttt tgtgaaacac ccgatctacg gtaaattcat caagcgtacg accaaactgc
     6661 acgtacatga cgagaacaac gaatgcggta tcggtgacgt ggttgaaatc cgcgaatgcc
     6721 gtccgctgtc caagactaaa tcctggacgc tggttcgcgt tgtagagaaa gctgttctgt
     6781 aatacagtac actctctcga tacgaataaa cggctcagaa atgagccgtt tattttttct
     6841 acccatatcc ttgaagcggt gttataatgc cgcgccctcg atatggggat ttttaacgac
     6901 ctgattttcg ggtctcagta gtagttgaca ttagcggagc actaaaatga tccaagaaca
     6961 gactatgctg aacgtcgccg acaactccgg tgcacgtcgc gtaatgtgta tcaaggttct
     7021 gggtggctcg caccgtcgct acgcaggcgt aggcgacatc atcaagatca ccatcaaaga
     7081 agcaattccg cgtggtaagg tcaaaaaagg tgatgtgctg aaggcggtag tggtgcgcac
     7141 caagaagggt gttcgtcgcc cggacggttc tgtcattcgc ttcgatggta atgcttgtgt
     7201 tcttctgaac aacaacagcg agcagcctat cggtacgcgt atttttgggc cggtaactcg
     7261 tgagcttcgt agtgagaagt tcatgaaaat tatctctctg gcaccagaag tactctaagg
     7321 agcgaatcat ggcagcgaaa atccgtcgtg atgacgaagt tatcgtgtta accggtaaag
     7381 ataaaggtaa acgcggtaaa gttaagaatg tcctgtcttc cggcaaggtc attgttgaag
     7441 gtatcaacct ggttaagaaa catcagaagc cggttccggc cctgaaccaa ccgggtggca
     7501 tcgttgaaaa agaagccgct attcaggttt ccaacgtagc aatcttcaat gcggcaaccg
     7561 gcaaggctga ccgtgtaggc tttagattcg aagacggcaa aaaagtccgt ttcttcaagt
     7621 ctaacagcga aactatcaag taatttggag tagtacgatg gcgaaactgc atgattacta
     7681 caaagacgaa gtagttaaaa aactcatgac tgagtttaac tacaattctg tcatgcaagt
     7741 ccctcgggtc gagaagatca ccctgaacat gggtgttggt gaagcgatcg ctgacaaaaa
     7801 actgctggat aacgcagcag cagacctggc agcaatctcc ggtcaaaaac cgctgatcac
     7861 caaagcacgc aaatctgttg caggcttcaa aatccgtcag ggctatccga tcggctgtaa
     7921 agtaactctg cgtggcgaac gcatgtggga gttctttgag cgcctgatca ctattgctgt
     7981 acctcgtatc cgtgacttcc gtggcctgtc cgctaagtct ttcgacggtc gtggtaacta
     8041 cagcatgggt gtccgtgagc agatcatctt cccagaaatc gactacgata aagtcgaccg
     8101 cgttcgtggt ttggatatta ccattaccac tactgcgaaa tctgacgaag aaggccgcgc
     8161 tctgctggct gcctttgact tcccgttccg caagtaaggt agggttacta aatggctaag
     8221 caatcaatga aagcacgcga agtaaaacgc gtagctttag ctgataaata cttcgcgaaa
     8281 cgcgctgaac tgaaagcgat catctctgat gtgaacgctt ccgacgaaga tcgttggaac
     8341 gctgttctca agctgcagac tctgccgcgt gattccagcc cgtctcgtca gcgtaaccgc
     8401 tgccgtcaaa caggtcgtcc gcatggtttc ctgcggaagt tcgggttgag ccgtattaag
     8461 gtccgtgaag ccgctatgcg cggtgaaatc ccgggtctga aaaaggctag ctggtaattg
     8521 tcaccaattg aatcacggga gtaaagacag atgagcatgc aagatccgat cgcggatatg
     8581 ctgacccgta tccgtaacgg tcaggccgcg aacaaagctg cggtcaccat gccttcctcc
     8641 aagctgaaag tggcaatcgc caacgtgctg aaggaagaag gttttattga agattttaaa
     8701 gttgaaggcg acaccaagcc tgaactggaa cttactctga agtatttcca gggcaaagct
     8761 gttgtagaaa gcattcagcg tgtcagccgc ccaggtctgc gcatctataa acgtaaagat
     8821 gagctgccga aagttatggc gggtctgggt atcgcagttg tttctacctc taaaggtgtt
     8881 atgactgatc gtgcagcgcg ccaggctggt cttggtggcg aaattatctg ctacgtagcc
     8941 taatcggagg aaaaaatgtc tcgtgttgct aaagcaccgg tcgttgttcc tgccggcgtt
     9001 gacgtaaaaa tcaacggtca ggttattacg atcaaaggta aaaacggcga gctgactcgt
     9061 actctcaacg atgctgttga agttaaacat gcagataata ccctgacctt cggtccgcgt
     9121 gatggttacg cagacggttg ggcacaggct ggtaccgcgc gtgccctgct gaactcaatg
     9181 gttatcggtg ttaccgaagg cttcactaag aagctgcagc tggttggtgt aggttaccgt
     9241 gcagcggtta aaggcaatgt gattaacctg tctctgggtt tctctcatcc tgttgaccat
     9301 cagctgcctg cgggtatcac tgctgaatgt ccgactcaga ctgaaatcgt gctgaaaggc
     9361 gctgataagc aggtgatcgg ccaggttgca gcggatctgc gcgcctaccg tcgtcctgag
     9421 ccttacaaag gcaagggtgt tcgttacgcc gacgaagtcg tgcgtaccaa agaggctaag
     9481 aagaagtaag gtaacactat ggataagaaa tctgctcgta tccgtcgtgc gacccgcgca
     9541 cgccgcaagc tccaggagct gggcgcaact cgcctggtgg tacatcgtac cccgcgtcac
     9601 atttacgcac aggtaattgc accgaacggt tctgaagttc tggtagctgc ttctactgta
     9661 gaaaaagcta tcgctgaaca actgaagtac accggtaaca aagacgcggc tgcagctgtg
     9721 ggtaaagctg tcgctgaacg cgctctggaa aaaggcatca aagatgtatc ctttgaccgt
     9781 tccgggttcc aatatcatgg tcgtgtccag gcactggcag atgctgcccg tgaagctggc
     9841 cttcagttct aaggtagagg tgtaagatgg ctcacatcga aaaacaagct ggcgaactgc
     9901 aggaaaagct gatcgcggta aaccgcgtat ctaaaaccgt taaaggtggt cgtattttct
     9961 ccttcacagc tctgactgta gttggcgatg gtaacggtcg cgttggtttt ggttacggta
    10021 aagcgcgtga agttccagca gcgatccaga aagcgatgga aaaagcccgt cgcaatatga
    10081 ttaacgtcgc gctgaataac ggcactctac aacaccctgt taaaggtgtt cacacgggtt
    10141 ctcgcgtatt catgcagccg gcttccgaag gtaccggtat catcgccggt ggtgcaatgc
    10201 gcgccgttct ggaagtcgct ggggttcata acgttctggc taaagcatat ggttccacca
    10261 acccgatcaa cgtggttcgt gcaactattg atggcctgga aaatatgaat tctccagaaa
    10321 tggtcgctgc caagcgtggt aaatccgttg aagaaattct ggggaaataa accatggcaa
    10381 agactattaa aattactcaa acccgcagtg caatcggtcg tctgccgaaa cacaaggcaa
    10441 cgctgcttgg cctgggtctg cgtcgtattg gtcacaccgt agagcgcgag gatactcctg
    10501 ctattcgcgg tatgatcaac gcggtttcct tcatggttaa agttgaggag taagagatgc
    10561 gtttaaatac tctgtctccg gccgaaggct ccaaaaaggc gggtaaacgc ctgggtcgtg
    10621 gtatcggttc tggcctcggt aaaaccggtg gtcgtggtca caaaggtcag aagtctcgtt
    10681 ctggcggtgg cgtacgtcgc ggtttcgagg gtggtcagat gcctctgtac cgtcgtctgc
    10741 cgaaattcgg cttcacttct cgtaaagcag cgattacagc cgaagttcgt ctgtctgacc
    10801 tggctaaagt agaaggcggt gttgtagacc tgaacacgct gaaagcggct aacattatcg
    10861 gtatccagat cgagttcgcg aaagtgatcc tggctggcga agtaacgact ccggtaactg
    10921 ttcgtggcct gcgtgttact aaaggcgctc gtgctgctat cgaagctgct ggcggtaaaa
    10981 tcgaggaata agtagcagat ggctaaacaa ccgggattag attttcaaag tgccaaaggt
    11041 ggcttaggcg agctgaaacg cagactgctg tttgttatcg gtgcgctgat tgtgttccgt
    11101 attggctctt ttattccgat ccctggtatt gatgccgctg tacttgccaa actgcttgag
    11161 caacagcgag gcaccatcat tgagatgttt aacatgttct ctggtggtgc tctcagccgt
    11221 gcttctatct ttgctctggg gatcatgccg tatatttcgg cgtcgatcat tatccagctg
    11281 ctgacggtgg ttcacccaac gttggcagaa attaagaaag aaggggagtc tggtcgtcgt
    11341 aagatcagcc agtacacccg ttacggtact ctggtgctgg caatattcca gtcgatcggt
    11401 attgctaccg gtctgccgaa tatgcctggt atgcaaggcc tggtgattaa cccgggcttt
    11461 gcattctact tcaccgctgt tgtaagtctg gtcacaggaa cgatgttcct gatgtggttg
    11521 ggcgaacaga tcactgaacg aggtatcggc aacggtattt caatcattat cttcgccggt
    11581 attgtcgcgg gactcccgcc agccattgcc catactatcg agcaagcgcg tcaaggcgac
    11641 ctgcacttcc tcgtgttgct gttggttgca gtattagtat ttgcagtgac gttctttgtt
    11701 gtgtttgttg agcgtggtca acgccgcatt gtggtaaact acgcgaaacg tcagcaaggt
    11761 cgtcgtgtct atgctgcaca gagcacacat ttaccgctga aagtgaatat ggcgggggta
    11821 atcccggcaa tcttcgcttc cagtattatt ctgttcccgg cgaccatcgc gtcatggttc
    11881 gggggcggta ctggttggaa ctggctgaca acaatttcgc tgtatttgca gcctgggcaa
    11941 ccgctttatg tgttactcta tgcgtctgca atcatcttct tctgtttctt ctacacggcg
    12001 ttggttttca acccgcgtga aacagcagat aacctgaaga agtccggtgc atttgtacca
    12061 ggaattcgtc cgggagagca aacggcgaag tatatcgata aagtaatgac ccgcctgacc
    12121 ctggttggtg cgctgtatat tacctttatc tgcctgatcc cggagttcat gcgtgatgca
    12181 atgaaagtac cgttctactt cggtgggacc tcactgctta tcgttgttgt cgtgattatg
    12241 gactttatgg ctcaagtgca aactctgatg atgtccagtc agtatgagtc tgcattgaag
    12301 aaggcgaacc tgaaaggcta cggccgataa ttggtcgccc gagaagttac ggagagtaaa
    12361 aatgaaagtt cgtgcttccg tcaagaaatt atgccgtaac tgcaaaatcg ttaagcgtga
    12421 tggtgtcatc cgtgtgattt gcagtgccga gccgaagcat aaacagcgcc aaggctgatt
    12481 ttttcgcata tttttcttgc aaagttgggt tgagctggct agattagcca gccaatcttt
    12541 tgtatgtctg tgcgtttcca tttgagtatc ctgaaaacgg gcttttcagc atggaacgta
    12601 catattaaat agtaggagtg catagtggcc cgtatagcag gcattaacat tcctgatcat
    12661 aagcatgccg taatcgcatt aacttcgatt tatggcgtcg gcaagacccg ttctaaagcc
    12721 atcctggctg cagcgggtat cgctgaagat gttaagatca gtgagctgtc tgaaggacaa
    12781 atcgacacgc tgcgtgacga agttgccaaa tttgtcgttg aaggtgatct gcgccgtgaa
    12841 atcagcatga gcatcaagcg cctgatggat cttggttgct atcgcggttt gcgtcatcgt
    12901 cgtggtctcc cggttcgcgg tcagcgtacc aagaccaacg cacgtacccg taagggtccg
    12961 cgcaaaccga tcaagaaata atcggggtga ttgaataatg gcaaaggcac caattcgtgc
    13021 acgtaaacgt gtaagaaaac aagtctctga cggcgtggct catatccatg cttctttcaa
    13081 caacaccatc gtgactatca ctgatcgtca gggtaacgcg ttgggttggg caacagccgg
    13141 tggttccggt ttccgtggtt ctcgcaaatc cactccgttt gcagctcagg ttgcagcaga
    13201 gcgttgcgct gacgccgtga aagaatacgg catcaagaat ctggaagtta tggttaaagg
    13261 tccgggtcca ggccgcgaat ctactattcg tgctctgaac gccgcaggtt tccgcatcac
    13321 taacattact gatgtgactc cgatccctca taacggttgt cgtccgccga aaaaacgtcg
    13381 cgtataacgc ctcgttttcc aggtttgttg gagaaagaaa atggcaagat atttgggtcc
    13441 taagctcaag ctgagccgtc gtgagggcac cgacttattc cttaagtctg gcgttcgcgc
    13501 gatcgatacc aagtgtaaaa ttgaacaagc tcctggccag cacggtgcgc gtaaaccgcg
    13561 tctgtctgac tatggtgtgc agttgcgtga aaagcaaaaa gttcgccgta tctatggtgt
    13621 gctggagcgt cagttccgta actactacaa agaagcagca cgtctgaaag gcaacaccgg
    13681 tgaaaacctg ttggctctgc tggaaggtcg tctggacaac gttgtatacc gtatgggctt
    13741 cggtgccact cgtgcagaag cacgtcagct ggttagccat aaagcaatta tggtaaacgg
    13801 tcgtgttgtt aacatcgctt cttatcaggt tagtccgaat gacgttgtaa gcattcgtga
    13861 gaaagcgaag aagcagtctc gcgtgaaagc cgctctggag ctggctgagc agcgtgaaaa
    13921 gccaacttgg ctggaagttg atgctggcaa gatggaaggt acgtttaagc gtaagccgga
    13981 gcgttctgat ctgtctgcgg acattaacga acacctgatc gtcgagcttt actccaagta
    14041 aagcttagta ccaaagagag gacacaatgc agggttctgt gacagagttt ctaaaaccgc
    14101 gcctggttga tatcgagcaa gtgagttcga cgcacgccaa ggtgaccctt gagcctttag
    14161 agcgtggctt tggccatact ctgggtaacg cactgcgccg tattctgctc tcatcgatgc
    14221 cgggttgcgc ggtgaccgag gttgagattg atggtgtact acatgagtac agcaccaaag
    14281 aaggcgttca ggaagatatc ctggaaatcc tgctcaacct gaaagggctg gcggtgagag
    14341 ttcagggcaa agatgaagtt attcttacct tgaataaatc tggcattggc cctgtgactg
    14401 cagccgatat cacccacgac ggtgatgtcg aaatcgtcaa gccgcagcac gtgatctgcc
    14461 acctgaccga tgagaacgcg tctattagca tgcgtatcaa agttcagcgc ggtcgtggtt
    14521 atgtgccggc ttctacccga attcattcgg aagaagatga gcgcccaatc ggccgtctgc
    14581 tggtcgacgc atgctacagc cctgtggagc gtattgccta caatgttgaa gcagcgcgtg
    14641 tagaacagcg taccgaccta gacaagctgg tcatcgaaat ggaaaccaac ggcacaatcg
    14701 atcctgaaga ggcgattcgt cgtgcggcaa ccattctggc tgaacaactg gaagctttcg
    14761 ttgacttacg tgatgtacgt cagcctgaag tgaaagaaga gaaaccagag ttcgatccga
    14821 tcctgctgcg ccctgttgac gatctggaat tgactgtccg ctctgctaac tgccttaaag
    14881 cagaagctat ccactatatc ggtgatctgg tacagcgtac cgaggttgag ctccttaaaa
    14941 cgcctaacct tggtaaaaaa tctcttactg agattaaaga cgtgctggct tcccgtggac
    15001 tgtctctggg catgcgcctg gaaaactggc caccggcaag catcgctgac gagtaaccgg
    15061 atcacaggtt aaggttttac tgagaaggat aaggtcatgc gccatcgtaa gagtggtcgt
    15121 caactgaacc gcaacagcag ccatcgccag gctatgttcc gcaatatggc aggttcactg
    15181 gttcgtcatg aaatcatcaa gacgactctg cctaaagcga aagagctgcg ccgcgtagtt
    15241 gagccgctga ttactcttgc caagactgat agcgttgcta atcgtcgtct ggcattcgcc
    15301 cgtactcgtg ataacgagat cgtggcaaaa ctgtttaacg aactgggccc gcgtttcgcg
    15361 agccgtgccg gtggttacac tcgtattctg aagtgtggct tccgtgcagg cgacaacgcg
    15421 ccgatggctt acatcgagct ggttgatcgt tcagagaaag cagaagctgc tgcagagtaa
    15481 tctgaagcaa cgtaaaaaaa cccgccccgg cgggtttttt tatacccgta gtatccccac
    15541 ttatctacaa tagctgtact ctttttgttc atcccctgga gtatttatgt ggttacttga
    15601 ccagtgggca gagcgccata tagcagaagc gcaagcgaaa ggtgagtttg ataacctggc
    15661 aggtagcggc gaaccattga tactggatga tgattctcac gtgccaccgg aattacgtgc
    15721 ggggtatcgc ttgctgaaga atgccggttg cttaccgcca gaacttgagc aacggagaga
    15781 agcaattcag cttctggata ttctcaaagg tatccgtcac gatgatccgc aatatcaaga
    15841 ggttagccgt cgattgtcat tactggaatt gaagctgcga caagctggat tgagtaccga
    15901 ttttttacgc ggcgattatg ctgacaagtt gttggacaaa atcaacgata actagtggag
    15961 tatgtatgta tcgcattggt gagctggcaa aaatggcgga agtaacacct gacacgattc
    16021 gttattacga aaaacagcag atgatggagc atgaagtgcg tactgaaggc gggtttcgcc
    16081 tgtataccga aagcgatctc cagcgattga aatttatccg ccatgccaga caactaggtt
    16141 tcagtctgga gtcgatccgc gagttgctgt cgatccgcat cgatcctgaa caccatacct
    16201 gtcaggagtc aaaaggcatt gtgcaggaaa gattgcagga agtcgaagca cggatagccg
    16261 agttgcagag tatgcagcgt tccttgcaac gccttaacga tgcctgttgt gggaccgccc
    16321 atagcagtgt ttattgctcg attcttgaag ctcttgaaca aggggcgagt ggcgttaaga
    16381 gtggctgttg attttttgca ctgacaggat tacactcgcg ccgttaaata accaactgga
    16441 gtttttatga gccgatatca gcatactaaa gggcagataa aggataatgc gatagaagca
    16501 ttactacatg atcccttatt ccgacagcga gtagagaaaa ataagaaagg gaaaggcagt
    16561 tacatgcgaa aaggaaaaca tggcaatcgg gggaactggg aggccagtgg caagaaagta
    16621 aatcactttt ttaccactgg tcttctgctt tcaggcgctt gttaagagcg gttattctgc
    16681 tctttcagca aatcacgaat ttctgccagt aatacttctt ctttagttgg tgcaggtgcg
    16741 gcagctggtt cttctttttt ccgattcagt ttgttgatta gcttaatcgc cataaagatg
    16801 gcaaaggcca caatcagaaa atcaaagacg ttttgaatga agacaccgta atgcatcaca
    16861 acagcaggga tacccccctg cgcatcgcgt agcgtgacag caaactgttt aaagtcgatc
    16921 ccgccaatta ataagcccag aggtggcatg atgatatcgg caaccagtga agatacaatc
    16981 ttcccgaatg ccgcaccgat aatgacaccc accgccaaat ccaccacgtt cccgcgcatt
    17041 gcaaattcgc gaaattcttt aataatgctc atgttattct ccctatgaag ccgacaacca
    17101 taagtctaac aaatgttaag ccattttcct gccaggaatg aatttaatta atccttgata
    17161 atcaaaggga aataataagg cgtcattaga cgccttatta attacaagaa gaaagggctt
    17221 ggctggaaga gtctttcgac gtcggtaata aactttttat ctgtgaggaa cataattacg
    17281 tgatcgcctt gctcaatgcg cagattgtca ttggcaatca tcacgtcgtt tccacgtacc
    17341 accgctccaa taatcgttcc tggcggtagc ttgatttcgt caatgactct gccgacaacg
    17401 cgtgaggtgc tttcatcacc gtgagcaacg gcttcaatag cttctgctac gccgcggcgc
    17461 aatgaggaaa caccaacaat atctgctttt cgcacatggc taagcaacgc agaaatagtt
    17521 gcttgttgtg gtgaaattgc aatatcgatc acgctcccct gaaccagatc cacataagcg
    17581 cgacgctgga tcaataccat cacctttttc gcacccatac gtttggcaag catggcggac
    17641 atgatattgg cctcgtcatc gttggtgacg gcaataaaca gatcaacttg atcgatatgt
    17701 tcttcggcca gcagttcttg atccgacgca tcaccaaaaa agacgatcgt attctgcaac
    17761 ttttccgcca gttcggcagc gcgctgctga ttacgttcga tgagtttaac gctgtaatct
    17821 ttttccagac gacgcgccag ccctgcaccg atattaccgc caccaaccag catgatccgc
    17881 ttatacggtt tttccagtcg ctgtaattca ctcatcaccg cgcggatatg ctgtgaagcg
    17941 gcaataaaga acacttcatc accagcttca acaatggtcg aaccttgtgg acgaatgggg
    18001 cgatcgtggc ggaaaattgc tgccacacga gtatcgatat gtggcatatg ttcgcgcatg
    18061 gtcgaaagtg cattaccaat cagcgggccg ccataatagg ctttaactac agcgaggctg
    18121 actttaccct cagcgaagtt caccacctgc aatgcgcctg gatactcaat cagtcggtaa
    18181 atattatcga taaccaactg ctctggtgcg atcagatgat caatcggtac agcatctgaa
    18241 tgaaatagct tatcggcatc gcgtacgtag tctggtgagc ggatgcgagc gatgcgatta
    18301 ggggtgttga aaagtgagta ggctacctgg caggcaacca tattggtttc atctgaactg
    18361 gttacagcaa ccagcatatc ggcgtcgtcg gcacctgcct cccgcaatac gcgtggatga
    18421 gagccatgcc cctgcacgac tcgcaggtca aatttatcct gcaaggtccg cagacgctca
    18481 ccgttggtat cgacaacagt aatatcgttg ttctcgccaa ccaggttttc cgccagtgtg
    18541 ccgccaacct ggccggcacc cagaatgata attttcatca gtcgcgaccc gttatctcat
    18601 cactttttga ttagcttagc gtaaaagaag ccgtcgccct cttcggcacc aggctgattt
    18661 tgtttacccg gttgctctgg tgttcctgtt tcgcaaagtt cggcatcagc ggtacgttgc
    18721 aaaaaggctt taatctgcag gctattctct tccggtaaca ccgaacaggt ggcatagacc
    18781 agggttccac cggattttaa atgcggccaa atggcgtcga gaatttcaga ctgcaattgc
    18841 gcgagttccg ggatatcgcg atcgcgacgt aaccatttaa tatctggatg gcgacgaatc
    18901 acccccgttg ctgaacaagg cgcatctaat aaaatgcgat caaactgttg ctcgccacac
    18961 cattgggaag ggtaacggcc atcaccttgt ttcacggttg ccttcatacc aaggcgtttt
    19021 aaattgtcgt aaacgcgaga gaggcgctgt tcgtcaatat caaccgcaac aacctgcgct
    19081 tctggtgcca cctcaaggat atgcgttgtt ttaccgccgg gggcggcaca aagatccaag
    19141 atgtgttcac cgttttgtgg cgcaagccag gtcatgcaac cttgtgctga tgcatcctga
    19201 acggtaaccc atccgtcttc aaaaccaggt agcgcatgaa caggtgcagg tgtttccagc
    19261 cgtacagcat cagggtaatc cgcatgcggg aaacctttca ttcctgcttc atccagcaat
    19321 gcaagccagc tgtcgcggga atgatgcgta cgattaatac gcagccacat tggcggacgc
    19381 tggttattgg cttcgacgat ggattgccac tgctctggat acgctttttg cagacgcttc
    19441 agcaaccagg aaggatgcag ataacgtgca tcacgggtat taaactcggc taataactct
    19501 tcttgccgac gctggaactg gcgtaataca ccgttaatca accctttaag ttgcggacgc
    19561 ttaattgcga tagcgccttc aaccgtttca gccagcgcag catgaggtgg aatgcgggta
    19621 taaagcagtt gatacaaacc aaccataatc aggtaatgca cagtgcgctg tttgccggtc
    19681 atcggacggg ccattaactt attaatcagc cagtctaact gcgaaagcgt acgcagtacg
    19741 ccaaagcaca actcttgaag aagtgctttg tctttatcgg aaactttttg ctggagcggt
    19801 ggcagaatgt tgcttaatga ttgcccttgc tcgacgactt gttcaacggc ctgggccgcc
    19861 atgctacgta aattacgttg ttttttcata agtataaaaa tgctcggcaa gaccgggctt
    19921 agaagagtgg actatcagac cagacggttg cccggaacaa accattcccg acgagagttc
    19981 aggaggtctt gcgcgctcat cgctttctta cccgcaggtt gtaacgagag caggttcagg
    20041 atgccatcac cagtcgcaac ctgaatgcct tgtttgttgg cttcaaggat cgttcctggt
    20101 gcagcgttgg ttgccgtatc aatgaccgac gctttccaga ctttaaccgg ctgtccttca
    20161 atttccagcc agctcattgg ccatgggttg aaagcgcgaa tgcagcgttc aagctgtgct
    20221 gccgaaagtg accagtcaat acgcgcttct tctttactca acttctcggc gtaagtgaca
    20281 agagtttcgt cctgaacttc tggtttcgcc gtgccgtctg ccaattgttt caacgtggtg
    20341 ataagccctt gtgggccaag ctctgccagc ttgtcgtaca gcgtaccact ggtatcttct
    20401 gcagtaatcg ggcaggagag cttatagagc atgtcaccgg tgtctaaacc aacatccatt
    20461 tgcataatgg ttacaccagt ttctgcatca cccgcccata gtgagcgttg gattggtgca
    20521 gcaccgcgcc agcgtggcag cagtgaacca tgaacgttga tacagccaag acgcggcatc
    20581 tccagcacag ctttaggcag aattaaacca taggcgacga cgaccataac atcagcctgc
    20641 aaatcggcga ccagttgctg gttttcttgt ggacgcaggg aaacaggttg aaaaacgggc
    20701 agaccttctt cctcagccag aactttaatc gggctgggca tcagtttttt accgcgtcct
    20761 gccggtcggt ctggctgggt gaacacgcca acgacgttat gaccagaaga caacagcgcg
    20821 tcgagatgac gcgctgcaaa gtcaggtgta cccgcaaaaa taatacgtag tgattctgac
    20881 acgttagttc ttatccttaa gcccgggctt tcagacgatc cagtttttca actttctgac
    20941 gaattcgttg ttgtttcagc ggtgacagat aatccataaa cagtttgcca accaggtgat
    21001 ccatctcatg ctgaatacag atagctaaca gaccctctgc ttccagttca aatggtttac
    21061 cgtcgcggtc aagggcgcga attttaactt tctctgcgcg cggcactaaa gcacgttgtt
    21121 cagggatcga caggcaacct tcttcaatgc ctgtttcgcc gcttttttct aaaagctccg
    21181 gattgattaa caccagccgt tcgtcacggt tttccgaaac atcaataaca atgatacgtt
    21241 gatggatatc aacctgggtt gccgccaggc caataccttc ttctgcgtac atcgtctcga
    21301 acatatcatc gacgatacgc tgaatttctg cattcacttc ttctaccggt ttagcaactt
    21361 tgcgaagccg ctcgtccgga atatgtaaca cttgcaaaac tgacataaat ctccagagat
    21421 gtgttcagga gttagaaaga ttatttcttc tattctagac aaatccccct ctgattgaca
    21481 gcatcactga ccaatcgcaa agattgctaa ggcttgctta tggcagggag ataaggatgg
    21541 tcgatacaga aatttggctg cgtttaatga gcatcagcag cttgtacggc gatgatatgg
    21601 tccgtatcgc tcactggctg gcaaaacagt cgcatattga tgcggttgta ttgcagcaaa
    21661 cagggcttac attgcggcag gcacaacgct ttctttcatt tccacgaaag agtatcgaaa
    21721 gctcactttg ttggttggag caacccaacc atcatttaat ccctgcggac agcgaatttt
    21781 atcctcctca acttcaggcg acgacagatt accccggcgc actgtttgtt gaaggagaac
    21841 tgcacgcgct gcattcattt cagcttgccg tagtggggag tcgggcgcat tcatggtatg
    21901 gcgagcgatg ggggcgatta ttttgcgaaa ctctggcgac gtgtggagtg acaattacga
    21961 gtggactggc gcgtggaatc gatggtgtgg tgcataaagc ggccttacag gtaaatggcg
    22021 tcagcattgc tgtattgggg aatggactta ataccattca tccgcgtcgc catgcccgac
    22081 tggctgccag tctgtttgaa cagggtggcg ctctcgtctc ggaatttccc ctcgatgttc
    22141 caccccttgc ttacaatttc ccacgaagaa atcgcattat cagtggtcta agtaaaggtg
    22201 tactggtggt ggaagcggct ttgcgcagtg gtttgctggt gacagcacgt tgtgcgcttg
    22261 agcaggggcg tgaagttttt gccttgccag gtccaatagg gaatccggga agcgaagggc
    22321 ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga accggaagaa attctggaaa
    22381 acttgcaatt tggattgcac tggttgccag acgcccctga aaattcattt tattcaccag
    22441 atcagcaaga cgtggcattg ccatttcctg agctcctggc taacgtagga gatgaggtaa
    22501 cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt gccagaggta gttactcaac
    22561 tactcgaact ggagttagca ggatggatcg cagctgtacc cggcggctat gtccgattga
    22621 ggagggcatg ccatgttcga cgtactaatg tatttgtttg aaacctatat tcacactgaa
    22681 gctgagttgc gtgtggatca agacaaactt gaacaggatc ttaccgacgc aggttttgag
    22741 cgagaagata tctacaatgc cctgctatgg ctggaaaaac ttgctgatta tcaggaaggg
    22801 ttggcagaac cgatgcaact ggcctcggat cctctctcca tgcgtattta tacaccggaa
    22861 gagtgtgaac gactggatgc cagctgccgt gggtttctgc ttttccttga gcagattcag
    22921 gtgctcaacc ttgaaactcg tgaaatggtg atagagcgag tgctcgcgct ggataacgca
    22981 gagttcgaac tggatgatct gaaatgggtg atcctgatgg tgttgttcaa catcccgggc
    23041 tgcgaaaatg cgtaccagca aatggaagaa ttactctttg aagtgaatga aggtatgctg
    23101 cattaatcat ttctttaatt cagcataagt tgttatggca aaatcagcac tgttcacggt
    23161 gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg gttattcgat ccggaaaaca
    23221 cggtccgttt cttggatgct cacagtatcc ggcgtgtgac tacgtccgtc ctctgaaatc
    23281 ttcagcggat ggacatattg tcaaagttct ggaggggcag gtttgccccg tatgtggcgc
    23341 aaatctggta ttacgccagg gacgctttgg tatgtttatt ggttgcagta actaccctga
    23401 atgcgaacat accgaactta tcgataaacc ggacgaaaca gcaattacat gcccccaatg
    23461 tcggacgggc catctggtcc agcgccgctc ccgttatggc aaaacatttc actcctgtga
    23521 tcgctacccg gagtgtcaat ttgccattaa cttcaaacca atagctggag aatgccctga
    23581 gtgtcattat ccgctactca tcgaaaagaa aaccgcgcag ggtgtaaaac acttttgtgc
    23641 cagtaaacaa tgtggaaagc cggtttcggc ggaataataa cgtgaataat aacctgcaag
    23701 gagacgctat cgcagctgcg atagatgtcc tcaatgaaaa acgtgtcatc gcctatccaa
    23761 cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga aacagcagtg atgagactgt
    23821 tggagttaaa acagcgtccg gttgataagg ggctgatttt aatcgcggca aattacgagc
    23881 agcttaaacc ctatattgat gacaccatgt tgactgacgt gcagcgtgaa accatttttt
    23941 cctgctggcc aggtcctgtc acctttgtct ttcccgcgcc tgcgacaaca ccgcgctggt
    24001 tgacgggccg ctttgattcg cttgctgtac gagtcaccga ccatccgttg gtggttgctt
    24061 tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc caacttgagt ggattgccac
    24121 cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc ggcgttcccg gttgtgcctg
    24181 gtgaaacggg ggggcgttta aatccttcag aaatccgcga tgccctgacg ggtgaactgt
    24241 ttcgacaggg gtaacataat ggaaacctat gctgtttttg gtaatccgat agcccacagc
    24301 aaatcgccat tcattcatca gcaatttgct cagcaactga atattgaaca tccctatggg
    24361 cgcgtgttgg cacccatcaa tgatttcatc aacacactga acgctttctt tcgtgctggt
    24421 ggtaaaggtg cgaatgtgac ggtgcctttt aaagaagagg cttttgccag agcggatgag
    24481 cttactgaac gggcagcgtt ggctggtgca gttaataccc tcaagcggtt agaagatgga
    24541 cgcctgctgg gtgacaatac cgatggtgta ggcttgttaa gcgatctgga acgtctgtct
    24601 tttatccgcc ctggtttacg tattctgctt atcggcgctg gtggagcatc tcgcggcgta
    24661 ctactgccac ttctttccct ggactgtgcg gtgacaatca ctaatcggac ggtatcccgc
    24721 gcggaagagg tggctaaatt gtttgcgcac actggcagta ttcaggcgtt gggtatggac
    24781 gaactggaag gtcatgagtt tgatctcatt attaatgcaa catccagtgg catcagtggt
    24841 gatattccgg cgatcccgtc atcgctcatt catccaggca tttattgcta tgacatgttc
    24901 tatcagaaag gaaaaactcc ttttctggca tggtgtgagc agcgaggctc aaagcgtaat
    24961 gctgatggtt taggaatgct ggtggcacag gcggctcatg cctttcttct ctggcacggt
    25021 gttctgcctg acgtagaacc agttataaag caattgcagg aggaattgtc cgcgtgaatc
    25081 aggccatcca gtttccggac agggaagagt gggacgagaa taaaaaatgt gtatgttttc
    25141 ccgctctcgt gaatggtatg caactgacat gcgcgatctc tggcgagagt ctggcgtatc
    25201 gctttactgg agatacgcca gaacagtggt tagcgagttt tcgtcagcat cgctgggacc
    25261 tggaagaaga agcggaaaac ttaattcagg aacaaagtga agatgatcaa ggctgggtct
    25321 ggttaccctg atccagatat tcgtccttcc atttcacgta attattcgcg gaatagcgta
    25381 acccagcctt ctcttcatca cttaacgggc ggatctgttt gacggggcta ccgagataca
    25441 gatatccgct ctccagccgt ttattttgtg ggaccagact acccgcacca atcatcacat
    25501 catcttctac tattgcgcca tcaagtaaaa ttgagcccat cccaaccaaa actcgattgc
    25561 caatggtgca gccgtggagc atcaccttgt gaccaacagt gacatcttcg ccaatggtta
    25621 atgggttgcc atctgggttg tacgaggatt tatgagtgac atgcaacata ctgccatcct
    25681 ggatattggt gcgtgctccg atctgtacat aatgtacatc tccacgaatc acaacgagcg
    25741 gccagatccc cacatcatca gccagacgaa cgtcaccaat cacgacactg ctatcgtcga
    25801 tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta tgggcgtaaa acatcagaca
    25861 tacttacctc agcaataaat gatttactaa tgactttggg ggcattattg gccttgtgca
    25921 agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgat tgcagcaaaa agggtgaaaa
    25981 aacaacaaac agaaaaaaag atcaaaaaaa tacttgtgca aaatattggg atccctataa
    26041 tgcgcctccg ttgagacgac aacgtgaaac acttcacagg atggtcggaa caacgaagag
    26101 aaaaaatcct gaaattcagg gttgactctg aaagaggaaa gcgtaatata cgccacctcg
    26161 cgacagtgag ctgaaagc
//
LOCUS       DADLSA010000045        25652 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.045, whole genome shotgun sequence.
ACCESSION   DADLSA010000045 DADLSA010000000
VERSION     DADLSA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.045"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..602)
                     /locus_tag="JN691_001881"
     CDS             complement(<1..602)
                     /locus_tag="JN691_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7243339.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALVVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            1050..2021
                     /locus_tag="JN691_001882"
     CDS             1050..2021
                     /locus_tag="JN691_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7243340.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKNLLLPRLSEFLYQHPGIELELSSHNRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            2070..3083
                     /gene="yhjD"
                     /locus_tag="JN691_001883"
     CDS             2070..3083
                     /gene="yhjD"
                     /locus_tag="JN691_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAY7243341.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            3494..4816
                     /locus_tag="JN691_001884"
     CDS             3494..4816
                     /locus_tag="JN691_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY7243342.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(4998..7058)
                     /locus_tag="JN691_001885"
     CDS             complement(4998..7058)
                     /locus_tag="JN691_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY7243343.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLTPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(7128..7895)
                     /gene="pdeH"
                     /locus_tag="JN691_001886"
     CDS             complement(7128..7895)
                     /gene="pdeH"
                     /locus_tag="JN691_001886"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAY7243344.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQLLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            7908..9056
                     /locus_tag="JN691_001887"
     CDS             7908..9056
                     /locus_tag="JN691_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7243345.1"
                     /translation="MFKSDAARTRQRLSARKGRTLPPGRGKFPHSTTESFRNTFWLKK
                     IMEHCFNMVDQQTTTAQTNANFLQIRFTTMSKKIAVIGECMIELSEKGADVKRGFGGD
                     TLNTSVYIARQVDPAALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGL
                     YYIETDSTGERTFYYWRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTS
                     REKLLSLLRECRAKGGKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDA
                     LWGQQPVEDVIARTHNAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTA
                     AGDSFSAGYLAVRLTGGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(9152..10648)
                     /locus_tag="JN691_001888"
     CDS             complement(9152..10648)
                     /locus_tag="JN691_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY7243346.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADSLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(10869..12155)
                     /locus_tag="JN691_001889"
     CDS             complement(10869..12155)
                     /locus_tag="JN691_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAY7243347.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVEVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(12339..14327)
                     /gene="hmsP"
                     /locus_tag="JN691_001890"
     CDS             complement(12339..14327)
                     /gene="hmsP"
                     /locus_tag="JN691_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAY7243348.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVNAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(14409..17882)
                     /gene="bcsC"
                     /locus_tag="JN691_001891"
                     /pseudo
     CDS             complement(14409..17882)
                     /gene="bcsC"
                     /locus_tag="JN691_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            complement(17864..18970)
                     /gene="bcsZ"
                     /locus_tag="JN691_001892"
     CDS             complement(17864..18970)
                     /gene="bcsZ"
                     /locus_tag="JN691_001892"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAY7243349.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTGPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQDWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(18977..21316)
                     /gene="bcsB"
                     /locus_tag="JN691_001893"
     CDS             complement(18977..21316)
                     /gene="bcsB"
                     /locus_tag="JN691_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAY7243350.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNKFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLSTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(21327..23929)
                     /gene="bcsA"
                     /locus_tag="JN691_001894"
                     /pseudo
     CDS             complement(21327..23929)
                     /gene="bcsA"
                     /locus_tag="JN691_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            complement(23926..24678)
                     /gene="bcsQ"
                     /locus_tag="JN691_001895"
     CDS             complement(23926..24678)
                     /gene="bcsQ"
                     /locus_tag="JN691_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAY7243351.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKANGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(24690..24878)
                     /locus_tag="JN691_001896"
     CDS             complement(24690..24878)
                     /locus_tag="JN691_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243352.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            25151..>25652
                     /locus_tag="JN691_001897"
     CDS             25151..>25652
                     /locus_tag="JN691_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY7243353.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRS"
BASE COUNT         6015 a         7101 c         6460 g         6076 t
ORIGIN      
        1 caggaggaga tatttaacat cattgccgcc tgggtgcgat ttttcacttc cagacggcga
       61 tacagggatt ccagatgcgc ttttaccgtt ccggtactga tattcagcgc tctgccgatc
      121 tccttatttg attcgcccgc cgctaacatg gttaaaatct cccgctggcg ggcgcttaac
      181 gatttgagat ctttaatgtc cttttccggc gtcgtccgcc agtctccagg cagaaacatc
      241 atccccatcg ccgcactatt taccaccaac gcaaatgtct cgacggttga atcgcgaggc
      301 acaatggcca gcacattaaa atggataact tcctgtaacc accgtttatt gcaatccgtc
      361 gccgtgatta acaccttaac ctcaggaaac tgcaccacgg ttttttgcag caaccagtag
      421 caaaactcac catcctgatc gccatcgagc ataactaagg cttcagggta actttccagc
      481 ttttgccata actcgtctgc ctgactgacc ccctgaatac tcactcctgg aatacgctgc
      541 tgtaaactga ttttcattcc atgaataaat attgactgcc tgtcaaacat gactatttgc
      601 ataactgaat ctccacctga atacgttaaa aagacttaag tagtggaagg gtattacccg
      661 cgagaaaaaa taagaattcg ccatttggcg gtggccattc tacagagatg acgcgtagaa
      721 aatagttacc gatataaata gttacagcta aacgcctgaa attacatgtc gagggcacta
      781 tttaaaacaa ttttgaggat ttccttatat tggtggttag tacgcatgca attaaaaatg
      841 aaattccgcg actacaagcc aaaataacaa atggcaatga gacaaaaata agcacaaata
      901 gccaatacgt cctctattca ctttaaaggg aatcactgaa aaatacgctc tgtttaaggg
      961 gattcacctt tctcataaag ctattccgcc cttttcctgc tgagaaatcg ccacattcgt
     1021 catgacaaca ttgtgaaacc cggcattaga tgttagaaaa aacaataaac aatgcgatat
     1081 gcgcgttact gtttcgctgt gaacaacaat cggtcaaaga aatggataaa attcacgcaa
     1141 tgcagttgtt catcaaagtc gcggagctgg aaagtttttc ccgcgcggcg gatttctttg
     1201 ctttgccaaa gggaagtgtt tcgcgccaga tacaggcact ggaacatcaa cttggcaccc
     1261 agcttctcca gcgcactacg cgacgggtca aactcacgcc agaaggcatg acctattatc
     1321 aacgggcaaa agatgtgttg agtaatctca gcgaactgga cggtctgttt caacaggatg
     1381 ccaccagtat cagcggtaaa ttacgcatcg acatcccgtc aggaatcgcg aaaaacctgt
     1441 tactgccgcg tctgtcggaa tttctctatc agcatccggg aattgagctg gaactgagta
     1501 gccataaccg tccggtagat attcttcatg atggttttga ttgcgtgata cgcactggcg
     1561 cgttaccgga agatggcgtt atcgcccgtc ccctcggcaa actaaccatg gtcaactgtg
     1621 ccagtccgca ctatctgacg cgctttggtt atccgcaaag ccccgatgat ctgacttcac
     1681 acgcaatagt gcgttataca ccgcacctgg gtgtacatcc gttaggtttt gaggttgcca
     1741 gcgttaatgg cgtccagtgg tttaagtctg gcggcatgtt gacggtaaac agtagcgaaa
     1801 actatctcgc cgccggtctt gccggtctgg ggattattca gatcccgcgc attgccgtgc
     1861 gcgaagccct gcgtgccggg cggcttattg aagtgttgcc aggctaccgt gccgagccgc
     1921 tctccctttc gctggtttac ccgcagcgtc gggagctttc ccggcgtgta aacctgttta
     1981 tgcagtggct ggctggcgta atgaaagagc acctggactg accgactata ctttttaaga
     2041 atgaccacga cagaaggaca aaagagcgga tgacgcagga aaacgagatc aaacgtccca
     2101 cccaggatct ggagcacgag ccgattaagc agttggataa tagcgagaaa ggcggcaaag
     2161 ttagccaggc gctggaaacc gtcaccacca ctgccgaaaa agtccaacgc caaccggtta
     2221 ttgcgcacct tattcgcgcg acagaacgct ttaacgatcg gctgggtaac cagtttggtg
     2281 cggctatcac ctatttctca tttttgtcga tgatcccgat tttgatggtg tcgtttgccg
     2341 cagggggctt tgtgctggcc tcccatccga tgttgctaca ggatatcttc gacaaaattc
     2401 tgcaaaacat cagcgatccg acactagccg ccacgttgaa aaacaccatc aacaccgccg
     2461 ttcagcagcg tacgactgta gggcttgtcg gcctggcggt ggcactttat tccggcatca
     2521 actggatggg taacctgcgt gaagcgattc gtgcccagtc gcgcgatgtc tgggaacgct
     2581 cgccgcaaga tcaggagaag ttctgggtaa aatatctgcg tgattttatt tcactgattg
     2641 gtttgttgat tgcgctgatt gtgacgcttt cgatcacctc ggttgccggt tcggcgcagc
     2701 aaatgattat tagcgccctg cacctgaaca gcattgagtg gctgaaaccg acgtggcgat
     2761 tgattggcct ggcgatttcc atcttcgcca actatctgct tttcttctgg atcttctggc
     2821 ggttgccgcg ccaccgtccg cgcaaaaaag ccctgattcg cggaacattt ctggctgcta
     2881 ttggttttga agtgattaaa atcgtgatga cctacaccct gccatcgctg atgaaatccc
     2941 cctctggcgc agcatttggt tccgtgctgg ggctgatggc atttttctac ttcttcgccc
     3001 gtttgacgct gttttgcgcg gcgtggattg ccaccgccga atataaagac gacccgcgaa
     3061 tgccagggaa aacgcagcct taaaataggc cggatgcggc gtaaacgcct tatccggcat
     3121 acgattttat tcggcctggc tccccgtagg ccggataaga tgcgccagca tcgcatccgg
     3181 ctataatgcg cacataacct cttgaaactc ttccccagag cctcttcagc catctatttg
     3241 ggagcaaaca atttcactcc aactcataac cccagcatat aaatccagtt ggtaactttt
     3301 atttaacctg aaaccagttt tatccactat ttataaaatt atgtgaagca tttcatagaa
     3361 gaaaaatcac tggcctaaac attatcccct ttttgcctgg tttttgacca tttccgcgat
     3421 ttgttacaca ttgaaatatc acttttgctg tgcgtaatat ggctattcgt tagccaaaaa
     3481 ataagaaaag attatgcaag caacagccac aacactcgac cacgaacaag aatacacgcc
     3541 gatcaactcg cgtaataaag tccttgtcgc ctctctcatt ggcacggcca ttgagttctt
     3601 cgacttttac atttacgcca ctgcggccgt tattgtgttt ccgcatatct tcttcccgca
     3661 gggcgatcca acggcagcaa cgctacagtc gctcgccacc ttcgccatcg ccttcgtcgc
     3721 gcgccccatt ggctctgccg tttttggtca ttttggcgat cgcgttgggc gtaaagcgac
     3781 gctggtcgcc tcgttgctaa cgatggggat ttcgaccgtg gtgattggtc tgctgccggg
     3841 ctatgccacg attggtattt tcgccccact gctgctggcg ctggctcgat ttggtcaggg
     3901 tctgggctta ggcggtgaat ggggcggcgc ggcgctgctg gcgactgaaa acgccccacc
     3961 gcgcaaacgt gcactgtatg gctccttccc acagctaggc gcaccgattg gcttcttctt
     4021 tgccaacggc actttcttgc tgctttcctg gctactgacc gatgagcagt ttatgagctg
     4081 gggctggcgt gtgccattta tcttctcggc ggtgctggtc attatcggcc tgtatgttcg
     4141 cgtgtcgctg catgagtcgc cggtgtttga gaaagtcgct aaagcgaaaa aacaggtgaa
     4201 gatcccgctg ggtacgctgc tgaccaaaca tgttcgcgta accgtactgg gtacgttcat
     4261 tatgctggca acctatacgc tgttttacat catgacggtc tactctatga ccttcagtac
     4321 cgccgccgcg ccagttgggc ttggcctgcc gcgtaacgaa gtgttgtgga tgctgatgat
     4381 ggcagttatt ggttttggcg tgatggtgcc agtcgccgga ttactggctg atgcctttgg
     4441 tcgccgtaaa agcatggtga tcatcaccac gctgatcatc ctgttcgcgc tgttcgcctt
     4501 taacccgctg ctcggttctg gcaacccgat tctggttttt gccttcctgc tgctggggtt
     4561 aagtctgatg gggctgacct tcgggccgat gggtgcgctg ttaccagagc tgtttccgac
     4621 agaagtgcgt tacaccggag catcgttctc ttacaacgta tcgtcgattc tcggggcttc
     4681 cgttgcgccg tatatcgcag cctggttgca ggctaactac gggttaggtg cggtggggtt
     4741 atatctggcg gcgatggctg gcttgacgtt aatcgccctg ctgctgaccc atgagacgcg
     4801 acatcagtcg ttgtaataag tgtgaaaagc ctgatgcgct acgcttatca ggactgaatt
     4861 tccatacaat atattgaatt cacaaggatt tgtaggccgg ataaggcgtt cacgccgcat
     4921 ccggcatgaa caaagcacac tttgccaaaa atctaaatct gtttaattgc cggatgtcag
     4981 acatccggca attatcatca cttcttcatc tgcgacaata tcgtccgaca ctgattagcc
     5041 tccccttcgg aaggtgagat cagtgccagt aacgccgccg ccggtgttac cagcgttgcc
     5101 agtgccgcag caacagcacc acgggcaatc agcggtccgg ctttcacccc agcctgcgga
     5161 tttttaaacg tcccacgcac atacagcggc gaacgcagtg tgataatccg aatcccttta
     5221 ctctccggat caatagtcaa atccagctgt tccgaagcaa aacttgccgt gccggtaaca
     5281 ttaatcaacg cgttctcagt atcgaaagca aaaatctgcg ggcgcgccac gccgttggca
     5341 atattcagat tcgccgccgc gcagttcacc cgcacctcat cgtcaccaaa tatcgcaccg
     5401 acaatgtagt tgccgacatt cagcccaaca atctccatca ggttgcggct caccagcccg
     5461 tcattcatca acagtttcag gttgccgtta ctgttgccta aaagcgccgc caccgagtta
     5521 ccgctaccgc gtagttccgc gtcaccgttc atttccccca gcgtcttctg catcagttcc
     5581 acatcgggca tcagttcttt cagtttcagt cgacgagcct gaatatctgc ccgcccctgc
     5641 atcggctttt tatcgccttc cagatgaata ttcgccgcaa tgctgccgcc cgccatgcca
     5701 aatttcagcg gttgcaggcg caggtcagca tttttgagga tgatatgagt agaaagatcg
     5761 ctaatcggca ggctactgcc atgctcaatg cgccgccctt tgaagcgaac atcggcatcc
     5821 ataacgtccc atttatcggt ttcgaagcgg tcataaggca gcactttgcc cgcaggctga
     5881 acgctttttt cgcccttctt ctgttcagac cgtttcgact tttctgcccc tttcccggaa
     5941 tcaacgccaa tcaacggtcc caggtccgcc agccgcaatt gccgcgactc gacatcacct
     6001 tccagttttg gtcgtggctt tccggtggtg tagaccagag aaccgtggat atcgctatcg
     6061 ccaattcgcc cattaaaacc gcgataatca aagaccgacg atttttcagt gtcgattttc
     6121 gctaccagcc gaccatccgt ttcaaacggc ggggtatcgg gcagcagaac gcccgtcagt
     6181 tcatagagat cacccagtga atcgccagaa aatttaagcc gtaaatcgac accgcccatc
     6241 ttcattgggt cattcacgac gccatcaaaa gcaacacggg tattacctga acggaaatca
     6301 gcctgtaccg gaaacggcgt cccttcgcca cgcagcgcca gcataccgcc tatttttccc
     6361 gtacccgtga gcggttcacc gttataacgt ccctgcgcct tcaggccaaa aatgtaatcg
     6421 cccacctttt ctttatccgc tttacctttc gatccagtaa cttcgctgaa tggcagcggc
     6481 ttgcctaacg gatcaacaaa aatttccaga tccgctttgc ttactttgtc atcaatggcg
     6541 atccgccctt gatcgaaaag aatattatcc agccgaaacg accatgccga cggctttgca
     6601 ttcgcgtctt tgttgtcatc gttggcaaga ttaaacgtcc agttattgtt cttttcagag
     6661 aggcgaatca ggcgcgcgtc gggcttttcg agcttgatcc acggcagcca gaccgttttg
     6721 gtcagcagcg ccagcggtgt cagcgttgcc tctactcgcg gcaaatgcac catcgtgact
     6781 tcgggaatat ccggtggatt accaagaatg atgtcttctg catgtacatg aggccacggc
     6841 acccagctgc gccagccagt ttcttgtttt tgccgctccc acaccacgcc cagatcgcca
     6901 cggatagcga acggacgatt caactccgca gagactttct ggttgatggt cggtttgagt
     6961 cgattccagt caaaagttgc aatcaaaatg atcgccacga caatcaacaa caagaaagcc
     7021 cctgaaatcg cagcggttat tttgcctgcc ttgctcatcg ttcgcttcct catattcttc
     7081 ctgtgccagt cctaaagata gtccagcccg gcggaaaatg aggcagctta tagcgccaga
     7141 accgccgtat tcagcgtttc catcggtgcc gggcgtgaaa gaaaccagcc ttgtgcggcg
     7201 aatgcgggcg agttctgaac atcacgccac tcttccggtg tttctacgcc ctcgacaatc
     7261 accccgcgac aatagcgatt cattagatgt aaaagctgag aaaagagtgt gcggccttcc
     7321 ggcgactgac gcagcatcac aaacagttct cgcgcgattt tgatgtagtc ataacgcact
     7381 tcacttagtg cagagaaatt tgccatcccg gtaccaaaat catccagcca cagcgggcca
     7441 aattcacaca tcgaggcaaa ggttgaatct ttcggcagac ggatatgctc caccagttcg
     7501 aaacgcagcc agggaagacg ctcaatctgg cgcaggattt ttggttgctg acgcagggcg
     7561 atgagcgtag ggccatcaat attgaccgat gccagcaggc cgtgctctac aaagaagtcg
     7621 gctttttgcg ccagcaaatc aatctgctct ttcacaacct ccatccgatg gctgacggtg
     7681 atttcagtaa aatagcgatc cggcggcagg agttgcgaag ggttcaaggg atgcgtgacc
     7741 accgttaata gctccacggc cattaaccgc ccgcatgttt gatagatcgg ctgccaggtg
     7801 taagcacgct cacactgcaa ccaaaaacgc cgttcctgca agctctcgat gcttgcttca
     7861 gggttgctta ttcgctggat aacctgcctt atcatcaaag atgtcctgtg tttaaaagtg
     7921 atgctgcccg gactcgacaa agattatcgg cgcgtaaggg cagaacttta ccgcctggcc
     7981 ggggtaaatt tcctcactcc acaacggaat ctttcaggaa tacgttctgg ctcaaaaaaa
     8041 taatggaaca ctgttttaat atggttgacc agcaaaccac cacagcgcaa actaacgcta
     8101 attttttaca gatcaggttc acgactatgt ccaaaaagat tgccgtgatt ggcgaatgca
     8161 tgattgagct ttccgagaaa ggcgcggacg ttaagcgcgg tttcggcggc gataccctga
     8221 acacttccgt ctatatcgcc cgtcaggtcg atcctgcggc attaaccgtt cattacgtaa
     8281 cggcgctggg aacggacagt tttagccagc agatgctgga cgcctggcac ggcgagaacg
     8341 ttgatacttc cctgacccaa cggatggaaa accgtcttcc gggcctttac tacattgaaa
     8401 ccgacagcac cggcgaacgt acgttctact actggcgtaa cgaagccgcc gccaaattct
     8461 ggctggcgag cgagcagtct gcggcgattt gcgaagagct ggcgaatttc gattatctct
     8521 acctgagcgg gattagcctg gcgatcttaa gcccgaccag ccgcgaaaag ctgctttccc
     8581 tgctgcgcga atgccgcgcc aaaggcggaa aagtgatttt cgacaataac tatcgtccgc
     8641 gcctgtgggc cagcaaagaa gagacacagc aggtgtacca gcaaatgctg gaatgcacgg
     8701 atatcgcctt cctgacgctg gacgacgaag acgcgctgtg gggtcaacag ccggtggaag
     8761 acgtcattgc gcgcacccat aacgcgggcg tgaaagaagt ggtggtgaaa cgcggggcgg
     8821 attcttgcct ggtgtccatt gctggtgaag cgttagttga tgttccggcg gtgaaactgc
     8881 cgaaagaaaa agtgatcgat accaccgcag ctggcgactc tttcagcgcc ggttatctgg
     8941 cggtacgttt gacgggcggc agtgcggaaa acgcggcgaa acgcgggcac ctgaccgcaa
     9001 gtaccgttat tcagtatcgc ggcgcgatta tcccgcgtga ggcgatgcca gcgtaaacgc
     9061 agcatatgca aaaggctgga taaagtgtta cccctctatc cagctcatcc ataaatacct
     9121 gatggttgtg ctaactcatc accatcaggc attactgtgc aggtggaata tccgtcactt
     9181 caggatgtac gtcatccgtg gcgacagagg tggtcgcagc ggcggtagat ggggccatga
     9241 tttgatccca ggccgcctgc aacgctttca tgttaaattc cggctcgcct ttcggctgca
     9301 gcagtattaa cgccatatca ttcgacaact gctgacgcag atcctgattt aacatctcca
     9361 cagtcaggct gttaaggaaa tcctgccgca gtttctgata ctgctccggc gcgatatcga
     9421 cgacctgatt ttgcaacgaa cgcatccgct ggcccatcag aatatcggta tcagcccgtg
     9481 cataggcggc aaacagtttc tgcagctcca gtttcttttg cgccactaac gcattgaact
     9541 cttcttccgg cagaccttta tcgcgaacct tcgccagttc acgcgccacc agattaaggt
     9601 tgctgttcag cttgtcgttt ggtgattcga tgttgatggc acactgcgca cgcagataca
     9661 gcacacggca gtcaaatcca agaccgatgt ctttgctgtc actggcgctt aacgcttgct
     9721 gaacatgcca gaacagcgcc tcgcgggcca ggtccgcacg ccagtagcgc agcagtgcgg
     9781 ctgattcacg aatcggctgc cacggcgtat cccacatgat agataaccgg tcctgacgca
     9841 ccgcgtcagt cataatactc accgcttccg cacgcagcgg agaaagcgtc ggcaccggag
     9901 ctggcgtttc acgtttgcct ttcagttcgc caaacgtttt gttgatttgg tcgacaaccg
     9961 agcgcgcatc cacgtttccc accaccagta gcgtcattgc atccggggtg taccatttct
    10021 gatagaaatc tttaattttt tccgcttcaa cgggttgttt cagcgaatcg gcaggatcgt
    10081 gacctaacaa ggttgaccct ttcagacgat agcgccacca gccctcttta gtatcggcag
    10141 gccaggttgc caccatgtcc tgactttgca gcgcgtggtt gatggtttct ggtgtgatgg
    10201 tcaatttgcc tgtggcattt gccagataag agagcgcttc tttcagcaaa tcgttacggt
    10261 tattgggcaa actcagatta aacagcgtgg tgtcataaga gacaattacc ggcggcatcg
    10321 ggcgtttagg gtcgatcccc tgctgccaca atgaacgcgc ctgtgctgcg tcaaggccac
    10381 cgctttgcgt tagcgcaata cgagggatgg cgtgactgta accgctctgt tgtgtacttt
    10441 cggcgagcga accggtatta accagcaggc gaatttcaac acgatcgctg ggacgctggg
    10501 gggtggtcag cacttgccac tgtaaaccgt tggaaagcgt cccctgttgc catgctggat
    10561 caggctggag cgcatctgcc tgcacatagc cagcagtggc catcatcagc aaaccgcccg
    10621 ctaaaagtcg aatttttatg ccctgcatgt gaacccctga tcaactatcc tggtaataaa
    10681 aaaactgccc gttgtcggtc agtctaaatg acgttaaaaa cacttcgtgt tagaccgcaa
    10741 gaacatgaaa atgtcacgga agaagtgaaa taaacctgaa ctcacccagg tggtaagttc
    10801 aggcacaggg gtcaattatg cgcaaacacc cgcactcggg gaagggagtg cgggcataag
    10861 tggtgagatt aagaggataa ttcgtgcgtt ttgccatccg gcgcacgatt attcagcaca
    10921 tcgtccagtt ttttgtggtc cagttctttc acccacttag caacgacaat ggtcgctacg
    10981 ccgttaccga ccaggttagt tagcgcacga gcttctgaca taaagcggtc gataccgagg
    11041 atcagcgcca gacccgctac cggcaaatgg cccaccgcag agagcgtcgc cgccagcacg
    11101 ataaagccac tacccgttac ccctgccgcc cctttagaag aaagcagcaa cacgattaac
    11161 agcgtgattt ggtggacgat atccatctga ctgttagtgg cctgggcgat aaacaccgcc
    11221 gccattgtca ggtatatcga tgtgccatca aggttaaacg agtagcctgt cgggatgacc
    11281 agccccacca ccgatttacg gcagccgagt ttctccatct tgtcgagcat acgcggcagc
    11341 gccgactcgg aagatgaagt ccccagtaca atcagcagtt cttcacggat gtagcggata
    11401 aatttgaaga tactgaaacc agtcgcttta gcgattgaac ccaataccag caccacaaac
    11461 aggatacagg taatgtagaa acagataatc agctgcccca gttgcaccag tgtgccgacg
    11521 ccgtatttac cgatggtaaa cgccattgcc ccgaacgcac caataggtgc cagacgcatg
    11581 atcatattga tgatgccgaa gatgacctgc gagaaacttt cgatcacgtt aaaaatcagt
    11641 tggcctttgc tgcccagacg gtggagcgca aaaccaaaca gtacggcaaa cagcagcacc
    11701 tgcagaatgt taccgctggc aaatgcgcca atgacgctcg ccgggatgac atccataatg
    11761 aaggcgacaa tgccctggtc tttcgcctga tcggcgtaaa cctctaccgc tttcgcatca
    11821 agcgttgccg gatcgacgtt cattccggca ccaggctgca cgacgttaac gatgataaga
    11881 ccaataatca gcgcgatggt actgacaatt tcaaagtaaa gcagtgcgac tgcgccggta
    11941 cgaccgaccg ccttcatgct ttccatgccc gcaatgcccg ttacgacggt acaaaagatg
    12001 acaggagcga tgatcatctt aatgagctta acgaagccgt cgccaagcgg tttcatttgc
    12061 tcgcctattt caggatagaa atggccaagg agaataccaa tggctatcgc tgtcaggacc
    12121 tgaaagtaaa ggcttttaaa cagagaggtt ttcatagggt gtcctttagt aaaaccacag
    12181 gtcttgtaag gttatggggg tacctgcggc cttaaaataa cacccagaca acatcacaga
    12241 aatgtacctg gatcataatt gaaacaaaaa ggttaaaaag tttgagctgg ctcgcacaaa
    12301 ccagcacttt ttaaagtttt gtaatcagtt tggggtagct acttttcttc caggtaactc
    12361 tcttcgaaga tttcaatagg gagtgggcga gcaaaaagga agccctgggc aataccaacg
    12421 cccgcttttg ccagccagtc tcgttgtgct tcagtctcca cgccttcggc aatcatttgt
    12481 aagttcaggc tctgcgccag catgataatt gcagcaatca tgctgctatc ttccggcaag
    12541 ccttcaacaa acattttgtc gattttcagt acgtcgattg gcaacgattt catatgctgc
    12601 agctgacgca gccctgcgta gcccatgccg aaatcatcca gcgccacccg aactccggca
    12661 ttgcgcagcg gacggaggat tgccaccgca gcatgagggt cgtcaatacg tcggctttct
    12721 gtcacttcca gaatcagtgt tcccggctga atgcgatagc gggttaacag ttccagcata
    12781 tccgccacca tattcgggtg catcagttgc agcgcagaga ggtttaccga caagggcagc
    12841 ataatgccgc gctcttgcca ggctgcaagc aatcgacagg actcttccag cacccagtga
    12901 ccgacggtaa ccatcagccc acagcactca atgcgatcga ttaagccatc cggcaggtcc
    12961 caactgccat ccggttgctg gatacgcagt aacacttccg cattgaccag tttaccgctg
    13021 gtcatctcga cctgtggctg taaccaaatt gcaaactgat gattttccag tgcattaagg
    13081 atatcgctct cttccgtcaa ccgcttctgg gctgcttcca tctgctgcgg atcaaagaac
    13141 tgaatctgat tcttgccttt atggcgagcg gtaaatgccg cagaaatagc gcgactgtaa
    13201 agctgttcgg cggtgagatc gccgtagaac atcgccacgc caatgctaca gtgcggacgg
    13261 agttgaatac gttcaatcgg caggcgctcg ctcatgatag tgagtacttg ctgacccaaa
    13321 gtgattgcgt gccacggttc ctgtacaccg ttggcaatga cagcaaagtc ataaccgcta
    13381 atctgcgcga ggatcatacg tggcgacagt accgatttga gtttttccac cagcgtcagc
    13441 agcagaattt ctcgttgcgc ctctttcagc acgcccgcag tatcacgcag ggtttcacag
    13501 gtgatgatca tcagcgcggt ggtttgttta cgcgcaacaa cctgctccag catctccatc
    13561 agcaaggctt tgttcggcaa atccgacacc gggaagcgca tcgcattttc gttctgttct
    13621 tcataatggc gctgcagcaa ttgctggttg aggttgtaac tgcgcaccaa cataccgatt
    13681 tcatcgtcct gatgcagacg cggtaatgcc agttggtgac caacaagctc ctgggctggg
    13741 atggcgttaa gttcgcgagc aatattgcgt aacggatgca aaatcaggcg gttaatgcac
    13801 cagctgatgg cgacggtcag gataagcgac aaaagtaagt aaatggtcac taacgttgag
    13861 agagtgctca tcacgaactt atacatacgg aaggaatccg cctgtagcac cagataggct
    13921 attggctgcg gatttgccgg acgttcgagc gagtagacgc ccagcgagat ttgaaccggt
    13981 agctcaaaca ggcgagtaac cattaccggc accggacgct ctggaataaa acttttacgc
    14041 agcgcctgga actggttagg cagcactaca tcggcacggc tgaccacgcc tgccggttta
    14101 atgctggcaa gaatagcttc cgcttcggga atatcgcctt tcaaaatagc tgaagataag
    14161 ggttcgcgga cagagcgagc aatgctttcc agttgcgtag ccgtgttata gcgattctgc
    14221 tggaccagat ggaacagcaa aacggtgcaa aaaataaaaa cgaacaccag gacaacggct
    14281 gccaccattg ccatctgctt gattgttaac gagcgactta cgcgcaaatt gtctcccccc
    14341 ggaaatccca gattgccgcc cgagtatacc cgatcgcagc ggcattaaga gaggcgctat
    14401 ctgaaaactt accagtcggc gtaaggtatc agcggctgcg gcggtaaatc catgtcaccc
    14461 tgccatccgg cggcggaata acgtacgtag agcagggaat ggctgggtgc gtaatctttc
    14521 gcctgctgga tatcaattgc cgtgccaaca aaccagttgg aagtaacacg tcgttcaagt
    14581 aatgcccgcg ccgtgtagcc gaagccctga ctgctgccgc catcgttgga ttgccgcgca
    14641 gcttcttcct gccagtcggt cgggatcaga ttcatcagcg gataacgcgg catggttttg
    14701 gtgcgtgaat gcgaccacga gccagacgcc cccagctccc acgaccagtt ttccgtgcgc
    14761 tcccgccaca tcaccggtat ggcaaacgac aggtattcct gcggactgta gtaaccgccc
    14821 tgaccgagtg agtagccact cagatctttg tcgtaatgcc agatcatgtt gttcaggccg
    14881 attgtgacgc ggcgattgtt ctggttaatg accttatagt aatagcccgt catccagcgc
    14941 acgcgccagt tatcttcgac atttttaccg gttaactggt cgccactaag cgatgcccag
    15001 acgccgtttg cttcaccttt atcgtagctc agacttagcc ccacaccgtc ggcacgtacg
    15061 ccaccccatt ttttcccggt attgctcggg gagtcttttt gcccaccaaa ggccagcaaa
    15121 gaactggaga tgggccgacg gtgggcgtta acggtgtaac ccagcggccc gatatcatcg
    15181 ctgtaactga tgccgccgac cacatccacc acgttgaagc ccatcggcgt ggtaccgata
    15241 tcccagctcc agacgtcatt tcgccagccg accgccacgc tggcaccgga atccgactgg
    15301 ctgcggttgc cgctacagtc ctgtaatgta caggtgcccc agttgtcatc ccatttgcca
    15361 tcggcattag tggagaaact gccgacgttc atattgacga aatcactgcg aaagaacatc
    15421 cgcccgtcag aatacggcgc atccacctgc aacatggtag tgtgcgcttt cagatcggag
    15481 taaccaccgg tgccgctcga accccagtaa tcgtgctcaa gggtgacgtt aagatcctgc
    15541 tggcgataga ggtccgccgc atcgctgcgc acgccgcgtt tcagccagtc atctttctcg
    15601 tcgttacggg tcagtcgggt aaaggtgtcg ttatcctgcg gacgcgtcgt agtcacaccg
    15661 gatgcgacca tggcgtcttt gtaggtttcc agcgcctgct ttggatcgcc cgcctgcgct
    15721 tcaaacttcg caccgtcgcg cagcaccatc gcgctttcca tcgacggtgg ctgagatttt
    15781 gcctgcggga tcaacttatt aaacgtctgc tgcgccgctg cggtatctcc aagctgcgcc
    15841 tgtgccagcg ccacgcgccg ctgagtgttc agcgaggtgt tatcggtggc aggcagtttc
    15901 gccagctggc tacgtgccgc cgctttgtca ccggcagcaa tatccacttc cgtcagacca
    15961 agaatggcat cggcgttagt tggctcccgc gtcaggacat tctgatatgc agtgcgggcg
    16021 gcggtgtaat cacgtcgttg ttacgcccag tcagccagcg tgaggtcaat acgcgtggaa
    16081 ggtggttgct gacgcagcat cgcttccgct tcagcctctt tgccgctttc tcgcaggcga
    16141 ttagcggttt ccagcacctg atcgctttgc agtcgattaa ccagctcctg aatattgctg
    16201 ttccactgcg cgcgcggcag gctgttgata tgcgccagcg ccgctctgtc ctggttgtga
    16261 cctgagaggt acagcccgta agcgtaaacc tgctccgggt cgttcggctt ctgctgcgcc
    16321 agattgcgca ttaacgtatc ggcctggctg cgttgtccgg cctgccagag atcctgcgaa
    16381 agtcggtaag taatccatac gctgccgggg tccagcgcca gtcgttgccg ctgaagtgct
    16441 gccgcctgcg cccatttgcc ctggttttcc agtacctctg cctgctgtgc cagacggtcg
    16501 ttttgcaggc tgcgttcgat atcatcaatg ctgcgccgct ggctggcaga gagcgaggcg
    16561 ataaacgctt cagctttttc tggcgactgc tggcggtaaa tattcgccag cccgcgcacg
    16621 gcattagtgt tgccgctgtc catccgcagg gtctgctgat aatagcgttc agcggcgggg
    16681 taatctttgc gcgccatcgc cacatcgccc agccccagca ccgcatagct gtcggtgtta
    16741 tcgacattac gtgcctgctg gaacaaccgt tctgcccggt caggattatt ggctttcagc
    16801 gcagcatcac cctgctggat cgccagccag tagcggttta ctttcagcag actgttccat
    16861 ttgtcgttgt tgctgctgtg cgggtccagt gcgagggctt tttccagatt cgccactgca
    16921 ttggcgcgat cgcctttctg agaatacgcc tggcccagcg cccccagagc ttcactgtct
    16981 ttcgggttcg cccgcaccgc ctgttgtagt tcgggaatgg ctttacccgc cataccagag
    17041 tccaccgccg ctaaaccttg cgcacgagcg cggaaagcag gatcggccag ctgtttttgc
    17101 tgttctgcca gttgcgattg cgcagccgcc acgctatcgc catcactaaa gatcgagaga
    17161 tattttttca gcgccgacac actggcatca ctgacgggca tgtctttaat ctgcccgtac
    17221 cagattttag aggccccttc gcgcccggcg ttcgatttcg ccatctgttc caggacggca
    17281 aaaccttcgt cacggcgatc gctactaaac agcaatagcg ccagattgtt ttgcaggccc
    17341 gtattgcccg gtgcatccgc attgatgcgt tttaactgat taatcgcttc gccacggcga
    17401 gccggaattt tcgccaccgt actccagtac tcgacagcaa tgtcaccttc cggcggcgca
    17461 ccgttgaaca gtttgttgta actcgccaca gcttcttccg catgaccggt agtcgcctgc
    17521 aatcttgcct gttgcagtgc ctgacgacca tccggcgtgg aaagtagcat cgtagtccgc
    17581 gacgatttat acgcatttga actcggcgct aactgcgaca gccgatcgag ctgtttttgc
    17641 gcgccatcaa tatcgccctg acgtaacaaa gaacggaaac gggcggcaac gacgtccggg
    17701 ttattcggat caataagttc cagccgatat aacgactgtt gcaccagatc ttcacgatgg
    17761 gtcgcttcgc ctaaccgaac ttgctccagc aactgttgct gagcggttgg tgctgcctcg
    17821 accatcggca tgacggccag accgagggaa agcgtgaata tgtttagtgt gaatttgcgc
    17881 attcctggcc ccagtcaggt aataactcac cttttgtcga gaagcggaaa cgatgttgat
    17941 cccagtcttg tccaaacagg gtcagcacat agttgtaata ggtatcgctg ccgggaaagt
    18001 tatcggccac gcgctggcgc tgaactgcct gcgcatcgcg gttttgtaaa aaaggcagca
    18061 tggcggcaga aaaaccgacc ggtcctttac cctgcgcttt ccccgtagcc acatccactt
    18121 tttccggcgg ataaccgttt ttctcagtga atgtcgccat cggtttaaac cggttgagca
    18181 tccgcgcttt ttgcggatcg ctgtcaggca tcatgcctac ccacatgtaa acgcggatag
    18241 cgtcgtagct gctgatcaat gttttttcgg cttttagctg ccagcctttg tctttctcat
    18301 agcgcaccca gtctggcgaa aagcctttcg gggcggtttc cagcaataaa cgttgattgg
    18361 tttcgcgcag cgtggtccac ggcgcgccaa agcgggtgaa atactgcgcc agcgtcggcg
    18421 gcatgtagct ggggttaaaa cgccagctgt tatcctcagc aaaacccact ttgcccggta
    18481 acaacatgga acccagccca ggcaccgtca ccacttcctc ccgcgcgata cgttttagca
    18541 acgcgctgcc gatgtcggta taacgctgct ctttccacaa acgccccgcc tccagcaacg
    18601 accaggccat ccagacatca ccatcggagg ccgaattgct gtccagcact tcccacttac
    18661 tgttctcttt cttgccccac agccaggcgg gcaaacgttc ttttaaagaa ccctgagcga
    18721 gattgttctg cgtccagtcg agaatattat cgaaagctgc acggtcgtta gccgccaggg
    18781 caaagaacat accgtaactt tgcccttcgg aggtggtgat tttgcgcgcg tcgctggggt
    18841 cgatgacgcg cccttcctga ctgatgtaat cctttttaaa ctgctcccag gcaggcccgg
    18901 tacaggctgc ctgaacacta aaggcagcca acaacagcat cgtcacgatt ccactacgca
    18961 acacattcat cttcaattac tcgttatccg ggttaagacg acgacgacta ataattcgca
    19021 gcagacgcca cagtacccat gccagcaata tcacactgat agccgccagc accgccagca
    19081 gaatcggatg gtttgccagc gcataccaca agcgctcgaa ccacggcaga tgacctacgt
    19141 aataaacgtc gccaacacgt aggctgttga taccggactc gcggatcacc gcgaccgaac
    19201 cgaacatggt ggcgcgtttg ccgctatcgt tcaccgcatc gttaagcatt tcataaccgc
    19261 gtgggctatc tgccaacagc gcaatcacgc tgcgctggtc gttatacggc gactggaagc
    19321 caatcaccgc cgccatcgca ccggaagagg tcagcgttga ctgggtttct gccgcgcgat
    19381 cgctctcgtc cggcacaatg ccggggaacg gggtctggcg catcggtgtt ttcacccagc
    19441 tttcggtcgc ctgcaccaat aggtcgatct gcttatcgtc tttcagtttg tccgggatac
    19501 caccgatgat catgatgtcg gcatctttgc cctgaatggt gctgccatca tcggtcaccg
    19561 tcagattaat cgccgggaag cccgtctgtg cgccgataaa accaacagta ctcagcaacg
    19621 tttccatctg tgcttcgtta ggcgctttcg gcatcacggt gatggtttgc gacagatccg
    19681 ccatccggct gaatgggaag cccgcgttag caaaggcgcg tagatccggc atcgggatga
    19741 agtggtaata cttcgagaag tcgatggtgg aatcgtcacc aatcaccaca tgattctgca
    19801 ccggctggaa ggtaatacag ttatctaccg aaccgcctgg catcgggttc atatactcaa
    19861 agtcgaagcg cagctggttg gtcgcgccca gtttcagcgc cggaatagag acatctgttt
    19921 tgccatccag caaaccttgt aataccggaa tacgcagcag cagacggttc gcctcctgtt
    19981 tgctgctcag gttgaaggat tgcaggaact tgttattcag gctgatatcc atccgcgaac
    20041 tgtctttcac cggcggcatg gtgtagcggt aattaatatc catatcaatg ccggtactgc
    20101 gcatcaggta gagatccggc ggcaggttta gcgaaacgtt aatcgctgct ggctcaaggc
    20161 cgctggattg taactgttct tcataggttt tcagttcgcc aaatgtgacc ggacgatcgg
    20221 tacgtaccca gttcggcgca tcgtacggct tacgcggtag cagcggtttc acttcattca
    20281 ctaccacgct ttcaccacgg aacagaatgt taccctgagc gatacctttc gctgcctgca
    20341 acaggtcttt gtcgtcacga ccaaacacca ccagcagttt gacgtaagga ttctgcggat
    20401 ggttaatcat ctcaatcacc ggggctttta ccgccggatg atcgcgcagg aagtccggcc
    20461 gtttgtcgtt ggttgcaaag acaatggcat tgcgatccgg caattggtta tagagcaccg
    20521 ggaagttctg cccacgccag ccagaacgcg aaccaaacca cgaggcgaca atggcagatg
    20581 cttgttgcag cccaacatcc ggcgcacccg caaagaccat cggcaaggtg ttagtacggt
    20641 tatcgcgcgg gtcaaagaat ggcaccggga agtgtgacag gtcattcttc acattcaggg
    20701 tctgataggt cagatccagt ccactgctcc gcccaacatc cagccaaagc gtggtgctgg
    20761 ccgggttttc gcacacgtcc tgataatggc cgacaaactc cagccgcaca cggttgaagt
    20821 cggtaataaa cagtggatta atgggcattt gcgccagcgt ttttttaccc aactgttctt
    20881 tggtcactgg cagcacgccc atcagctcat cattgagata aacctttaac tgcgactgga
    20941 caggcagtaa cgatggcgat ggcgtgtatt cgaggttgag catcgccttc gtcaccactt
    21001 catcgctgcg cataccaaac tcaatgctgc cgttcgggtt aataccacgt agcaccatgc
    21061 tgcccggcgg cggcgcgatt tgcgcaaagg tcagcttcac atcacgcgaa ggaccgttct
    21121 gcgccacgat gggggcatca gctccctgct cgcctgacat cacttgcccc acctgcggat
    21181 tttgttccgt ctgggcggct acagctggct cagcattgat cagtggttgc gttgctggcg
    21241 tcgcctgcgt catgaaagag gggaacgcac tcatccccat agccactgca caaatccaga
    21301 atagttttct tttcatcgcg ttatcatcat tgttgagcca aagcctgatc cgatggttgt
    21361 gccgtttcgc tccgctccgg gcggcgcgga ataaacgata caacccagga aaccagagaa
    21421 gtcagcacac ggaatatgcc cttcaccgaa gaaggcgcaa actccgccag atggcggtag
    21481 ccacggaagc cgagcttcag aatatccagc agactttcca gcggcttatc ttccgggtag
    21541 ctgtcctgcc agagcgccca tgtatccgca cgggcaaacg tacactgcac aaaatcgata
    21601 tgttgctggg tggtgagcgg cattaatttc agcccaactt cattacccat cacgcgcgcc
    21661 acctgggtcg ggaagacgta ttcctgctga ccgcgtttaa gcaacagatt cactttctgc
    21721 ccttccagaa tctgcgcctg accgttgatc ttgatcccca aaccaccgtc ggagaaatcc
    21781 tgaacggtac acgagaagag gtgaccatct tcgcgggcaa ttgccgcggg catcgtcatc
    21841 tccacgcggt gcgatcggcg tacctgtttg ctttctaccg ataccgcaac tgcgccgcca
    21901 agaacaatca ggttgtagaa cacccacacc atactgacga ccacggtgag catctcggtt
    21961 ggcgggccat agaagtagcg ccagatgcct accgcaacgc ccaccaggtt gagtagaaca
    22021 aggaagatgt agggccgcga gatcacccag tcgacgtact cttcttccac cagtccacct
    22081 ttggcggtga cgttaaattt gcctttgtgc gggttaatca gcgccaccag cgtcggtggt
    22141 gcgatatacc acgccagcac cgtttcgtag atttcactcc agaaagagtg gcgatggatc
    22201 ttcgagttgg tcaggctggc atggatcata tgcggcagca cgaatagggc gatcatcaac
    22261 gctggcgcat agatgatgta ggcatgaagc agcaggaacg ccagcggcgc agtcaggaag
    22321 atcagccgtg gaatgcccga caagaagtgg aacatggcgt tgacgtagca cagccgctga
    22381 gcaaacttca gtcctttacc ggtgagcggg ttatcgagac ggaagatttg taccatcccg
    22441 cgcgcccagc gaatacgctg accgatatgc gccgacagac tttcggtcgc cagccccgcc
    22501 gcctgcggaa tacgcatata cgcggaggta tagccacgac ggtgcaaacg cagagaagta
    22561 tgcgcatctt cagtcacggt ttcgacagca atgccgccaa tttcatccag cggcttacga
    22621 cgaatcaccg cacaggaacc gcagaagaaa gtggcgtccc acatatcgtt gccatcctgc
    22681 accagaccat agaacagcgt gccttcgttc ggcgttttac gaaaacgtcc caggttgcgt
    22741 tcaaacgggt ccggcgagaa gaagtggtgt ggagtctgca tcatcgccag ctgtttctct
    22801 ttcaggaacc agcccatggt catttgcagg aacgatcgcg ttggtacgtg gtcgcagtcg
    22861 aaaatcgaca cgaactcgcc tttggcatat ttcagcgcat tgttgatgtt gcccgctttc
    22921 gcatgttcat gagtggtgcg ggcgatatat ttcaccccca cgttttgcgc aaactggcga
    22981 aactcttccc tgccgccgtc atcgaggatc cagatgttca gtttgtcttt cggccagtcg
    23041 atacccagcg aggcgtaaat ggtatttttc accacattga gatcttcgtt gtaagtcggg
    23101 acaaagatat ccactgacgg ccacagcgac atatccttcg gcaatggcac cggctgacga
    23161 ttcagcggcc atactacctg gaagtagccg agcaccagca caatccacgc gtacgtttca
    23221 gcgaagagca gaataagccc gcacaccagg ctgaccggat cgtcccagtt cagcgtagag
    23281 gtgtaacgcc accagatata acggcaagaa acggtcagcg acagcacaat caacattagc
    23341 gccgagaagc gccccggcat ccgccgtacg atcagcgcta ccccccacag cagcatcagg
    23401 aagataaact gcgccagcgg gttaaacggc tgagtaacgc agattaacgc cagaatcagc
    23461 gagaaggtga cgatgatacc gaggatcaac cgccgcgccc cggcactcaa atgaccgagc
    23521 tcttttttct catccagatg ctgtgtttta tggctaacgc gctcaggcag ctcgttcatc
    23581 cattgatggt aacgtccacg aatattttgc agacctgaaa atgcccgctg cgcggtttcg
    23641 gcgtttcttt gcgcgatgca ccgatcaata accagcatgt ttgaatgaga taacggaccg
    23701 ggtccagcgg acgcgaggcg ttgatatgcg tatacaggtt tttatgttct gcgcgaatac
    23761 gctgccagcg cgggtgctcc agcggaataa aaatccaggc caggatcatc cagaaacagc
    23821 cgagcgtcgc gctgaaagcc gacgcaccgt gacgacgata atcgcgataa cgcccgataa
    23881 gccgcgcgtt gaccggcggg ataagcaacc accgggtcag gatactcatg atgcactccc
    23941 gactggcgtt ttcagcccgg agtagttcaa caggcaccag ttcgccagcg tcagtatctc
    24001 ttcagccgcc agcgcatcac tgcgatattc acctactggt tgcttagccg ccaggcattc
    24061 agccatcgct tcatcacgat gaatgagcat cggcagtaat cggcgctggc tttgcaacca
    24121 aagctggtaa atatcgtcct gaacctgact gccaatacgg aagtcattaa tcaaaatatg
    24181 tgcgccatcc ggcagcgctt gctgatgcag tcggatatgg cagttggcat ccacattgac
    24241 gattgccagc gagtgctcgc acaaactcag cagctggtgg gttatctgcg aggcatcacg
    24301 cggtaagtcg attaaaatcc actggtaacg cccgttggct tttagttgct gtaagccgga
    24361 gcaaatatcg ctcagccggg tttgccagtg ctgtggattt tcttgttctt caatggataa
    24421 ctgaccaaaa ggcagcaaat cgagctgcga ggtgtagcgc aacccagcgt cacgccagtc
    24481 ctggccatcc agcatcgctc tggcccagcc ctgacggtgg gtaaaatcaa cattaaatga
    24541 caggcgcaac aagttgtccg ggcaggcatc gaccaccagg acattttctc ccaacatttg
    24601 taatgaccag gctaatgcgg cggtgatggt tgttgtcccc acgcctcccc gcaccccctg
    24661 caatcccagt acggccattc accaatcccc tacttttgtt gcgcaaactc tgccagcaac
    24721 ggccagcgtt ttaatgccgc ggccaactgt tcgcgttggg aaatatcggc ataatcaata
    24781 tcaggcagtg aaaatgcctg ctttaacgcc acaatatcat tctggaagat atagcctatc
    24841 gcgggatcag gcagagtatc tggttcattg ttattcattt tgcttgatcc ctatgaaata
    24901 accgtaccgt tcatgagaca attctaaccg tgagcatttc gccggtttat tgtttacggc
    24961 gagacgggaa tttgctcact ttttgacttt ccgttacgca acagaaaaag ttaaaaacga
    25021 aaagactaaa atcacacgtc ttgtgacctt ctaaattgat tcacatgcta aatctgataa
    25081 gttttaattt caatggtagg tttatttctt agctttcgct agtaaactga taatcagaca
    25141 aaatggtgac ataagggaca ttgtggaccc tgtattctct atcggtatct catcattatg
    25201 ggatgagctg cgacatatgc cagcaggcgg cgtctggtgg tttaacgtcg atcgccatga
    25261 agatgctatc agtctggcga atcaaacaat tgcatcccag gctgaaaccg cacacgtcgc
    25321 ggtcattagc atggacagcg atccggcgaa aatctttcaa ttagatgatt ctcaagggcc
    25381 ggaaaaaata aaattatttt caatgctaaa tcatgaaaaa ggtctatact atttggcccg
    25441 tgatttgcag tgttctattg atccccataa ttaccttttt attcttgttt gcgcaaataa
    25501 cgcatggcaa aacattcctg ccgagcggct tcgctcatgg ttggataaaa tgaataaatg
    25561 gagcaggtta aaccattgtt cgcttttggt aattaatccc ggaaataata acgataaaca
    25621 attttcattg ttgcttgagg aataccgttc ac
//
LOCUS       DADLSA010000046        25496 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.046, whole genome shotgun sequence.
ACCESSION   DADLSA010000046 DADLSA010000000
VERSION     DADLSA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.046"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..537
                     /locus_tag="JN691_001898"
     CDS             <1..537
                     /locus_tag="JN691_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY7243354.1"
                     /translation="FAEKLQQAVSKLHIGDGLEKGVTIGPLIDEKAVAKVEEYIADAL
                     EKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLAPLFRFKDEADVIAQ
                     ANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVAPFGGIKASGLGREG
                     SKYGIEDYLEIKYMCIGL"
     gene            551..1831
                     /gene="gabT"
                     /locus_tag="JN691_001899"
     CDS             551..1831
                     /gene="gabT"
                     /locus_tag="JN691_001899"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HAY7243355.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTRHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            2154..3494
                     /gene="gabP"
                     /locus_tag="JN691_001900"
     CDS             2154..3494
                     /gene="gabP"
                     /locus_tag="JN691_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HAY7243356.1"
                     /translation="MLSIAGVIGASLFVGSSVAIAEAGPAVLLAYLFAGLLVVMIMRM
                     LAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFWVLVIPLEANIAAMILHSWVP
                     GIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKVIAILAFIFLGAVAISGIYPY
                     AEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAEIVTIAAAESDTPEKHIVRAT
                     NSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSVLELLNIPHAKLIMDCVILLS
                     VTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYY
                     APAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIG
                     FITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            3515..4177
                     /gene="csiR"
                     /locus_tag="JN691_001901"
     CDS             3515..4177
                     /gene="csiR"
                     /locus_tag="JN691_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HAY7243357.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(4178..4627)
                     /gene="kbp"
                     /locus_tag="JN691_001902"
     CDS             complement(4178..4627)
                     /gene="kbp"
                     /locus_tag="JN691_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAY7243358.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVLEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(4711..4869)
                     /locus_tag="JN691_001903"
     CDS             complement(4711..4869)
                     /locus_tag="JN691_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAY7243359.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            5052..5351
                     /locus_tag="JN691_001904"
     CDS             5052..5351
                     /locus_tag="JN691_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7243360.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            5361..5885
                     /gene="ygaP"
                     /locus_tag="JN691_001905"
     CDS             5361..5885
                     /gene="ygaP"
                     /locus_tag="JN691_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAY7243361.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(5932..6336)
                     /gene="stpA"
                     /locus_tag="JN691_001906"
     CDS             complement(5932..6336)
                     /gene="stpA"
                     /locus_tag="JN691_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAY7243362.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKFLDDFLI"
     gene            7004..7453
                     /gene="alaE"
                     /locus_tag="JN691_001907"
     CDS             7004..7453
                     /gene="alaE"
                     /locus_tag="JN691_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAY7243363.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(7490..7834)
                     /locus_tag="JN691_001908"
     CDS             complement(7490..7834)
                     /locus_tag="JN691_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAY7243364.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            7986..8315
                     /locus_tag="JN691_001909"
     CDS             7986..8315
                     /locus_tag="JN691_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY7243365.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            8563..8808
                     /gene="nrdH"
                     /locus_tag="JN691_001910"
     CDS             8563..8808
                     /gene="nrdH"
                     /locus_tag="JN691_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAY7243366.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPQAASA"
     gene            8805..9215
                     /gene="nrdI"
                     /locus_tag="JN691_001911"
     CDS             8805..9215
                     /gene="nrdI"
                     /locus_tag="JN691_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAY7243367.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            9188..11332
                     /gene="nrdE"
                     /locus_tag="JN691_001912"
     CDS             9188..11332
                     /gene="nrdE"
                     /locus_tag="JN691_001912"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAY7243368.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            11342..12301
                     /gene="nrdF"
                     /locus_tag="JN691_001913"
     CDS             11342..12301
                     /gene="nrdF"
                     /locus_tag="JN691_001913"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAY7243369.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            12656..13858
                     /locus_tag="JN691_001914"
     CDS             12656..13858
                     /locus_tag="JN691_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAY7243370.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            13851..14915
                     /gene="proW"
                     /locus_tag="JN691_001915"
     CDS             13851..14915
                     /gene="proW"
                     /locus_tag="JN691_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAY7243371.1"
                     /translation="MADQNNPWDTTPAAGSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            14973..15965
                     /gene="proX"
                     /locus_tag="JN691_001916"
     CDS             14973..15965
                     /gene="proX"
                     /locus_tag="JN691_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAY7243372.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KSGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            16157..17341
                     /locus_tag="JN691_001917"
     CDS             16157..17341
                     /locus_tag="JN691_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7243373.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCMTFANFSILWTSMAFLLAAPPFNYSDGDIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGATGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            17465..18202
                     /locus_tag="JN691_001918"
     CDS             17465..18202
                     /locus_tag="JN691_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAY7243374.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            18192..18527
                     /gene="ygaH"
                     /locus_tag="JN691_001919"
     CDS             18192..18527
                     /gene="ygaH"
                     /locus_tag="JN691_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAY7243375.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(18546..18647)
                     /locus_tag="JN691_001920"
     CDS             complement(18546..18647)
                     /locus_tag="JN691_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243376.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            18618..19148
                     /gene="emrR"
                     /locus_tag="JN691_001921"
     CDS             18618..19148
                     /gene="emrR"
                     /locus_tag="JN691_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAY7243377.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            19275..20447
                     /gene="emrA"
                     /locus_tag="JN691_001922"
                     /pseudo
     CDS             19275..20447
                     /gene="emrA"
                     /locus_tag="JN691_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            20464..22002
                     /gene="emrB"
                     /locus_tag="JN691_001923"
     CDS             20464..22002
                     /gene="emrB"
                     /locus_tag="JN691_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAY7243378.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRLAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(22066..22581)
                     /gene="luxS"
                     /locus_tag="JN691_001924"
     CDS             complement(22066..22581)
                     /gene="luxS"
                     /locus_tag="JN691_001924"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAY7243379.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(22731..24287)
                     /gene="gshA"
                     /locus_tag="JN691_001925"
     CDS             complement(22731..24287)
                     /gene="gshA"
                     /locus_tag="JN691_001925"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY7243380.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(24360..24788)
                     /locus_tag="JN691_001926"
     CDS             complement(24360..24788)
                     /locus_tag="JN691_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7243381.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(24785..25351)
                     /gene="yqaB"
                     /locus_tag="JN691_001927"
     CDS             complement(24785..25351)
                     /gene="yqaB"
                     /locus_tag="JN691_001927"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAY7243382.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         6062 a         6522 c         6526 g         6386 t
ORIGIN      
        1 tttgccgaaa aattacagca ggcggtgagc aaactgcaca tcggcgacgg gctggagaaa
       61 ggcgtcacca tcgggccgct gatcgatgaa aaagcggtag caaaagtgga agagtatatt
      121 gccgatgcgc tggagaaagg tgcgcgtgtg gtttgcggcg gtaaagcgca cgaacgcggc
      181 ggcaacttct tccagccaac cattctggtg gacgttccgg ccaacgccaa agtgtcgaaa
      241 gaagagacgt tcggccccct cgccccactg tttcgtttta aagatgaagc cgatgtgatt
      301 gcacaagcca atgacaccga gtttggtctt gccgcctatt tctacgcccg tgatttaagc
      361 cgcgtcttcc gcgtcggcga ggcgctggag tacggcatcg tcggcatcaa taccggcatt
      421 atttccaatg aagtggcccc attcggcggc atcaaagcct cgggtctggg tcgtgaaggt
      481 tcgaagtatg gcatcgaaga ttacttagaa atcaaatata tgtgcatcgg tctttaactg
      541 gagaatgcga atgaacagca ataaagagtt aatgcagcgc cgcagtcagg cgattccccg
      601 tggcgttggg caaattcacc cgattttcgc tgaccgcgcg gaaaactgcc gggtgtggga
      661 cgttgaaggc cgtgagtatc ttgatttcgc gggcgggatt gcggtgctca ataccaggca
      721 cctgcatccg aaggtggtgg ccgcggtgga agcgcagttg aaaaaactgt cgcacacctg
      781 cttccaggtg ctggcttacg agccgtatct ggagctgtgc gagattatga atcagaaggt
      841 gccgggcgat ttcgccaaga aaacgctgct ggttacgacc ggttccgaag cggtggaaaa
      901 cgcggtaaaa atcgcccgcg ccgccaccaa acgtagcggc accatcgctt ttagcggcgc
      961 gtatcacggg cgcacgcatt acacgctggc gctgaccggc aaggtgaatc cgtactctgc
     1021 gggcatgggg ctgatgccgg gtcatgttta tcgcgcgctt tatccttgcc cgctgcacgg
     1081 cataagcgag gatgacgcta tcgccagcat ccaccggatc ttcaaaaatg atgccgcgcc
     1141 ggaagatatc gccgccatca tgattgagcc ggttcagggc gaaggcggtt tctacgccgc
     1201 gtcgccagcc tttatgcagc gtttacgcgc tctgtgtgac gagcacggga tcatgctgat
     1261 tgccgatgaa gtgcagagcg gcgcggggcg taccggcacg ctgtttgcga tggagcagat
     1321 gggcgtggcg ccggatctta ccacctttgc gaaatcgatc gcaggcggtt tcccgctggc
     1381 gggcgtcacc gggcgcgcgg aagtgatgga cgctgttgca ccgggcggtc tgggcggcac
     1441 ctatgccggt aacccgattg cctgcgtggc tgcgctggaa gtgttgaagg tgttcgagca
     1501 ggaaaatctg ctgcaaaaag ccaacgatct ggggcagaag ctaaaagacg gattgttggc
     1561 gatagccgaa aaacacccgg agatcggcga cgtacgcggg ctgggagcga tgattgccat
     1621 cgagctgttt gaagatggcg atcacagcaa accggacgca aaactcaccg ccgagatcgt
     1681 ggcacgcgcc cgcgataagg gcctgattct tctctcctgc ggcccgtatt acaacgtgct
     1741 gcgcatcctt gtaccgctca ccattgaaga cgctcagatc cgtcagggtc tggagattat
     1801 cggtcagtgt tttgatgagg cgaagcagta gcaccgcccc catgcaggag gcgacgctgc
     1861 gcgtcttgtc cggcctacga gaatccaagt cggataaggc gtttacgccg catccgacaa
     1921 tctgtacgtg aacaggaata aatctatgtc ggccggataa ggcggagccg ctctccggca
     1981 aaaagaatca ataacaatta aacgcgtgac ccgacgcggg aaatgtcggg gcgctctccc
     2041 aagtgacaca ctttcgagag gattcaggat ggggcaatca tcgcaaccac acgagttagg
     2101 cggcgggctg aaatcacgtc acgtcacgtc acgtcacgtc acgtcacgtc accatgttgt
     2161 ctattgccgg tgttatcggc gcaagtctgt ttgtcggttc cagcgtcgcc atcgccgaag
     2221 cgggcccggc ggtattactg gcctatctgt tcgccggatt actggtggtt atgattatgc
     2281 ggatgctggc ggaaatggca gttgccacgc ccgataccgg ttcgttttcc acctatgccg
     2341 ataaagccat tggccgctgg gcgggctata ccatcggctg gttgtactgg tggttttggg
     2401 tactggttat cccactggaa gccaacatcg ccgccatgat cctgcactcg tgggttccag
     2461 gcattcccat ctggttattt tccctcgtca ttaccctcgc cttaactggc agtaatttat
     2521 taagcgttaa aaactacggc gaatttgagt tctggctggc gctgtgcaaa gtcatcgcta
     2581 tcctggcctt tattttcctt ggtgcagtcg caattagcgg tatttaccct tatgccgaag
     2641 tgagcgggat ctcaagattg tgggatagcg gcggctttat gcccaacggt ttcggtgcgg
     2701 tattaagcgc gatgttgatc accatgttct cgtttatggg cgcagaaatt gtcaccattg
     2761 ccgccgcgga atccgacacg ccggaaaaac atattgtccg cgccaccaac tcggttatct
     2821 ggcgtatttc tatcttctat ttgtgctcta tttttgtcgt cgtggcatta ataccgtgga
     2881 atatgccggg gctgaaagcc gttggatctt atcgctcggt tctggaattg ctcaatattc
     2941 cccatgcgaa attaatcatg gactgtgtga tattactttc cgtaaccagt tgcctgaact
     3001 cggccctgta taccgcctca aggatgctct actccttaag ccgtcgcggt gatgctcccg
     3061 cggtaatggg taaaatcaac cgcagtaaaa ccccgtacgt ggcggtgtta ctctccaccg
     3121 gcgcggcatt cttaacggtg gtggtgaact attacgcgcc tgcgaaggta tttaaatttc
     3181 tgatcgacag ctccggcgct atcgccctgc tggtttattt agtcatcgcc gtttcacagt
     3241 tgcggatgcg caaaattctg cgagcagaag gaagcgaaat tcgcttgcga atgtggcttt
     3301 atccctggct cacctggctg gtcatcggct ttattacctt tgtgttggta gtgatgctat
     3361 tccgtccggc gcaacagtta gaagtgatct ccaccggctt attagcgata gggattatct
     3421 gtaccgtgcc aattatggct cgctggaaaa agctggtatt gtggcaaaaa acacccgttc
     3481 ataatacgcg ctgataatga tcaggagtca caccatgacc attacgtctc tggatggcta
     3541 tcgctggctg aagaacgata ttattcgcgg taattttcaa ccggatgaaa aattacggat
     3601 gagtttactg acatcgcgtt atgcacttgg cgttgggccg ttgcgggaag cactttctca
     3661 actggtggcg gaacggctgg tcacggtggt gaatcaaaaa gggtatcggg tggcgtctat
     3721 gtcagagcag gagctgctcg atattttcga cgcccgcgcc aatatggaag cgatgctggt
     3781 cagtctggcg attgcccgcg gtggcgatga atgggaggca gacgttctcg caaaagcgca
     3841 tctgctcagt aagctggaag cctgtgacgc cagcgagaaa atgcttgatg agtgggatct
     3901 gcgtcatcag gcgtttcata cagcaattgt ggcgggctgt ggttctcact atttgctgca
     3961 aatgcgtgaa cggttgtttg atctggcggc gcgttatcga tttatctggc tgcggcgaac
     4021 ggtgctttcg gtggaaatgc tggaggataa acacgatcag caccagaccc tgactgcggc
     4081 ggtactggcg cgagatacag cgcgcgccag tgagttaatg cgccagcatt tactgacgcc
     4141 aattcccatt atccagcagg cgatggctgg caattaacta ctcttccgga atacgcaaca
     4201 cttgccccgg ataaatttta tccgggcttt ttagcatcgg tttattcgct tcgaagattt
     4261 tattgtacag attagcgtta ccgtagacct gtttggaaat ggcactcaga gtgtcgccag
     4321 acttaacggt ataaaactgg ctggcagtgg ctggtgtcgc cgttttcacc tgatcatcaa
     4381 cactggcaat accggaaata ttccccaccg caacaaggat tttctccttc gcctcctgac
     4441 tcaggccgtc accagtgacc gtcgctttgc cgtcggcaat ttgaatgttc actttatcgg
     4501 catcaggtat accggttttg ctcagatgct ccagcacctt cttcgcctga tcgtctttat
     4561 cgtgctgacc tgtaaccgcg tcccagagtt tttctccggc atctttcaca aaattgaaca
     4621 gacccatagc tacctcattc gttaacggaa acactcagaa agtgtagcag aggaacgcgg
     4681 cgcgttaacg ccgcgggggt gagcacaggt ttaatcgcgg gtttgcaccc agaacgcgtg
     4741 aatcaaaccg gggatatacc caagcagtgt cagcagaata ttaataatga acgcccaacc
     4801 gaacccttta ccgagcagca cgccgagcgg cggcagaatg atggtgatga cgattctcca
     4861 gaaacccata tgtactccct ataagaaaat tactcattgt ttaaaaagag attttatctc
     4921 ttaagcgtag taattttagc ggagcctgcc agtttcagct tacgctttta ctctcattta
     4981 cgctgctgag gctggtatat gttgcatttt tagtttagag tttacttaat ttagaaaata
     5041 cttaaataaa tatgactgaa ctcgcgcaat tacaggccag taccgaacag gcagcggcct
     5101 tattgaaagc aatgagccac cctaaacggt tgctgattct gtgcatgctt agcggttccc
     5161 ccggcaccag cgcgggagag ctgacgcgca ttaccggact gagcgcctct gcgacatcac
     5221 agcatctcgc tcgtatgcgg gacgaagggc ttatcaacag ccaacgggat gcccaacgca
     5281 ttctatattc cattaaaaat gaggcggtaa atgccattat cgccaccctg aaaaatgtct
     5341 attgtccgta aggagtcact atggctttga caaccatttc gccgcatgat gcacaagaat
     5401 taatcgcacg cggcgcaaag ttaatcgata ttcgtgacgc tgatgaatat cttcgtgaac
     5461 atattcctga agcagatctg gctccattat ccgtgctgga acagtcaggt cttccaccta
     5521 aattacgtcg cgagcaaatt atattccact gccaggcagg taaacgcacc agtaataacg
     5581 ccgataaatt agcagcgatt gccgcccccg cagaaatatt tttactcgaa gatgggattg
     5641 atggctggaa aagagctgga ttgccagtag cggtaaataa atctcaaccc ttgccgttaa
     5701 tgcgccaggt gcagatcgct gcgggtggtt taatattaat cggcgttgta ctgggttata
     5761 ccgtaaatag cggtttcttc ttattaagtg gctttgttgg tgccgggtta ctgtttgcag
     5821 gaatcagcgg tttttgtgga atggcaaggt tgttagataa gatgccgtgg aaccaacgag
     5881 cttgagaagc gacgccggac gcgccctagc agcgacatcc ggcctcagta attagatcag
     5941 gaaatcgtcg agaaatttac cttctgccag cgcctgagca attggcttcg gtgtacggcc
     6001 ctgaccggtc caggttttag tttcaccgtt aacatcagtg aatttatatt tcgccggacg
     6061 cggctggcgt tttttaccag cacgtggtgc agcagcagag ctattaccca ataactcttc
     6121 cgggttaatt ccgtcagctt tcatcagctc cagccaggtg ctaatttttt cctgacgctc
     6181 agccagttca cgctgctgct gttcttcttc ttcacgtctt tctttagtga caaccctgaa
     6241 tttttcgagc atttcttcaa gaacgtcaat ggagaattcg cgagccatcg cacggagggt
     6301 gcgaatgtta tttaaacttt gtaacattac ggacataata aagaaaacct tttaacgcca
     6361 aaacaaaaaa gtatttcgtc gcgtagtgta agcgatcact tattatattt ccacaaaatt
     6421 taatctattt acagtcctgc gcgagattat ttcagctgtt attttctgaa actctcagaa
     6481 taatttcaag attatatatt agccattttg atgagtaatt taggttattg gtgaacagag
     6541 tggcgctctc tgctcgctaa ctccgcaagc ctgacaaaac cgaagtgaat aaaaatgcaa
     6601 tataacagaa taaaagaaaa cacccctcac tggtgctttc tgctgttaat gtacattttc
     6661 tggcatcaaa aatgctgtaa atgacccggt cacgacaaac accgaattct ctacgcttat
     6721 agcgttacct cacccccaaa ctttcattag tgaaattaaa ggcaaggcca tatccaattg
     6781 atttaattga aaataaatca tatcagcatg taaaaatcca caatgtacaa aaaacgagcc
     6841 gttacggaat attttatcta caaaaactga ctaaataaaa atttttcact aattgattag
     6901 tcatagccag cgatatacgc tatgcgaaaa tgcagattgc aatgagatcc actgctttca
     6961 tctccattaa catcccatta cgcttttatt aaggagcatt agcatgttct caccgcagtc
     7021 acgcttgcgt catgcagttg cagatacgtt cgcgatggtt gtttactgtt ctgtcgtgaa
     7081 catgtgtatt gaagttttcc tctccggaat gagcttcgaa cagtcttttt attccagatt
     7141 ggtagcgatt ccggtgaaca tcttaattgc atggccatac ggtatgtacc gcgatctgtt
     7201 tatgcgcgcg gcacgcaaag ttagcccgtc gggctggata aaaaatctgg ctgatatcct
     7261 ggcttatgtg acgttccagt caccggtgta tgtggcgatc ttgttagtgg tgggcgcaga
     7321 ctggcatcag attatggcgg cggtcagttc aaacatcgtt gtttcgatgt tgatgggggc
     7381 ggtttatggc tacttcctcg attattgccg ccgactgttt aaagtcagcc gttaccagca
     7441 ggtaaaagcc tgactcttct tttcgcgact ggcatcgcca gtcgcgtctt taactggctt
     7501 cgccaaataa accattcaaa taacgttcaa gcgcaacacg cgaactaaag ccatgcggga
     7561 taccgtaatg ctcgtgtcgc tcgcctgcta aatagagcgg aaattgctgg taatgatcgc
     7621 aagttttaat atcggaagcg ggttcagcca gtgttgaact gcgttctttt aatgttggat
     7681 gaattaccag agcggtacgt cccattcgcg cttcgcgatt aacatagaca taattttccc
     7741 cacggcggta accatacgct ttctgggtta ccacatcgac agtaaaaccg actttttcaa
     7801 gtacgcgcgc cacctcgtct ggtcgtaaat acatatttga tcctcgttat tttcaaccgc
     7861 gggcctacct tacctgattg cgcattcaca acgctttcag aaaagtccat aaagccgcga
     7921 tgaacctcag ttaagaaaat tatggtctac actgaaaatt acatcgaatt ctaatggggg
     7981 atcatatgtt taaccgaccg aaccgcaacg acgttgatga tggcgtgcag gatatccaga
     8041 atgatgtcaa tcaattagct gacagcctcg aatctgtatt gaagtcctgg ggcagcgacg
     8101 ccaaagggga agctgaagcc gcacgcagta aagctcaggc gttgctgaaa gaaaccaggg
     8161 cgcgaatgca tggtcgtact cgcgtccagc aagccgcgcg cgatgccgtt ggctgcgcgg
     8221 attcttttgt tcgtgaaaga ccctggtgta gcgtgggtac agcagctgcg gtaggtattt
     8281 ttatcggcgc actgttaagc atgcgcaaat cgtagtgcaa aaatgataat aaatacgcgt
     8341 ctttgacccc gaagcctgtc ttcggggttt ctttttgcct ggtgaatcac aaaaatcccc
     8401 ctcccccgtc acgctcatat ctagggtaat ttcgatcact atttgctata tattgtgtgg
     8461 ttgaatcttt tttcaactac atctagtatc tctgtatcaa cagagagaca acccgacgcg
     8521 tatcatcgcg ccgtatcttc attttaaacg gaaatacgaa tcatgcgcat aactatttat
     8581 actcgtaacg attgcgttca gtgccacgcc accaaacggg cgatggaaaa ccggggcttt
     8641 gattttgaaa tgattaatgt cgatcgcgtt cctgaagcag cggaagcgtt gcgtgctcag
     8701 ggctttcgtc agttgccggt agtgattgct ggcgatctta gctggtctgg tttccgtccg
     8761 gacatgatta accgtctgca tccagcgcca caagcggcca gtgcatgagc cagctcgtct
     8821 acttctccag cagctccgaa aacacgcagc gttttatcga acgtttaggt ctgcccgcgg
     8881 tgcgcatccc gctcaatgag cgggaacgga ttcaggtaga cgagccttac atcctgatcg
     8941 tgccctctta cggcggcggc ggtacggctg gcgcggtgcc acgacaggta attcgctttt
     9001 taaacgacga gcacaaccgg gcgttgcttc gcggcgttat tgcttctggt aatcgcaact
     9061 ttggtgaggc gtatggccgc gccggagatg tgattgcccg gaaatgcggc gtgccgtggc
     9121 tgtaccgttt tgaactcatg ggtacgcaaa gcgatatcga aaacgttcgt aaaggagtaa
     9181 ccgaattttg gcaacgacaa ccgcagaatg cctgacgcag gaaacgatgg attaccacgc
     9241 gctgaatgcg atgcttaatc tttacgatag cgcaggtcgc attcagttcg ataaagaccg
     9301 ccaggccgtt gacgccttta ttgcgacgca tgtgcgtccg aacagtgtga ccttcagtag
     9361 ccagcagcag cgcctgaact ggctggtcaa cgaaggttac tatgatgaaa gcgttcttaa
     9421 tcgctactct cgcgactttg tcattacgct gtttgcccac gcacacacca gcggttttcg
     9481 tttccagaca ttcctcgggg catggaagtt ttacaccagc tatacgttga agacattcga
     9541 cggtaaacgt tatctggaag attttgccga tcgagtcacg atggtggcgc tgacgctggc
     9601 acaaggcgat gagacgctgg cgttgcaact gaccgatgaa atgctgtcag gacgctttca
     9661 gccagccacg ccaacattcc tcaactgcgg taagcagcag cgcggcgaac tggtttcctg
     9721 ttttttgctg cgtattgaag acaatatgga gtcgattggt cgggcggtaa attccgcact
     9781 gcagctgtcg aaacgcggcg gcggcgtagc atttttgctg tcgaatctgc gggaagcggg
     9841 cgcgccaatt aaacgtattg aaaatcaatc ttctggcgta attccggtga tgaaaatgtt
     9901 ggaagacgca ttttcctatg ccaaccaact cggcgcgcgt cagggggcag gtgcagtcta
     9961 tttacatgct catcatcccg atattctgcg ttttctcgac acgaaacggg aaaatgccga
    10021 cgaaaaaatc cgcattaaaa cactgtcgct gggcgtggtg atcccggata tcactttcca
    10081 tctggcaaaa gagaatgcgc agatggcgct gttttcgccc tatgacgttg agcgagttta
    10141 tggcaagccg tttgccgata tcgccattag cgaacactat gacgaactgg ttgccgatga
    10201 acgcatccgc aaaaaatacc tcaacgcccg tgatttcttc cagcgactgg cagaaatcca
    10261 gtttgagtcc ggctatccct acatcatgta tgaagacaca gtaaaccgtg ctaaccctat
    10321 cgccgggcgc ataaatatga gtaatctctg ctcagaaatt ttgcaggtta acagcgcctc
    10381 agagtatgac gagaatctcg actatgcccg cacaggccat gatatttcct gcaatttagg
    10441 ttcgttgaat attgcgcaca ccatggattc ccccgatttt tcccgcacgg tagagactgc
    10501 cgtgcgcggt ttaacggcgg tgtcagatat gagtcatatc cgcagcgtgc cgtccatcga
    10561 agccggaaat gccgcctcgc acgccatcgg actggggcag atgaatttac atggttatct
    10621 ggcgagggaa ggcatcgctt atggttcgcc ggaagccctg gatttcacca atctctattt
    10681 ctataccatc acctggcacg cactgcgtac ctcgatgttg ctggcgcgcg aacgcggtga
    10741 aaccttcgcc gggttcaaac agtcacgcta tgccagtggt gaatatttca gccaatatct
    10801 gcaaggtaac tggcagccga aaacggcgaa agttggcgaa ctgtttgccc gcagcggtat
    10861 tacgttacct acccgtgaga tgtgggcgca actgcgcgac gacgtgatgc gctacggcat
    10921 atacaaccag aatctccagg cggtgccgcc aaccgggtct atctcttata tcaaccatgc
    10981 tacgtcgagt attcatccga ttgtggcgaa agtagagata cgcaaagagg gcaaaacagg
    11041 acgcgtttac taccctgccc cgtttatgac taacgaaaat ctggcgctgt atcaggacgc
    11101 ttacgaaatt ggcgcagaaa agatcatcga cacctacgcg gaagcgactc gccatgtcga
    11161 tcaggggctg tcgctgacgc tgtttttccc cgataccgcc accactcgcg atatcaacaa
    11221 agcgcagatt tacgcctggc gcaaaggtat caaaacgctc tattacatcc gcctgcgtca
    11281 gatggcgctg gaaggcactg aaattgaagg ctgcgtctcc tgtgcacttt aaggaatatc
    11341 tatgaaactc tcacgtatca gcgccatcaa ctggaacaag atatctgacg ataaagatct
    11401 ggaggtgtgg aatcgcctga ccagcaattt ctggctacca gaaaaggtgc cgctgtcgaa
    11461 cgatattccc gcctggcaga cattaactgt cgtagaacaa caactgacga tgcgcgtttt
    11521 tactggcctg acgctgctcg acacgctgca aaatgttatc ggcgcgcctt cgctgatgcc
    11581 cgatgcgctc acgcctcatg aagaggcggt attatcgaat atcagcttta tggaagcggt
    11641 tcatgcccgc tcttacagtt cgattttctc gacgctgtgc cagaccaaag atgtcgatgc
    11701 cgcctacgcc tggagtgaag aaaacgcacc gttgcagcga aaagctcaga ttattcagca
    11761 acattatcgc ggtgatgatc cgctgaaaaa gaaaatcgcc agtgtgtttc ttgaatcttt
    11821 tttgttctat tccggtttct ggctgccgat gtatttttcc agccgcggaa agctgaccaa
    11881 taccgcggac ctgatccgtc tgattatccg cgatgaagca gtccacggtt actacatagg
    11941 ctataaatat cagaaaaaca tggaaaagat atctctggga caacgtgaag agttgaagag
    12001 tttcgccttc gatttgttgc tggaactcta cgacaacgag ttgcaataca ccgatgagct
    12061 gtacgccgaa accccgtggg ctgacgatgt gaaagcgttt ctctgttaca acaccaataa
    12121 ggctttgatg aatctgggct acgaaccgtt atttcccgca gaaatggcgg aagtgaatcc
    12181 ggcaatcctc gccgcgcttt cgccgaatgc cgatgaaaat cacgatttct tttccggttc
    12241 aggctcctct tatgtgatgg ggaaagcggt tgaaacagaa gatgaagact ggaatttctg
    12301 agggtgttat tttcaaaaat atcactaccc gcagcaggga aataattccc gccaaatagc
    12361 tttttatcac gcaaatattc tgtagtgatc tacactgata ctctgttgca ttattcgcct
    12421 gaaactacaa tattcaggcg ttttttcgct atctatgaca aaaaatatca acttttctcg
    12481 atttgctctc agcccttata tcacggaaaa ttccggcgat ttgctcgcat caatattcat
    12541 gccacatttg ccctcagggg ttgcctcaga ttctcagtat gttagggtag aaaaaagtga
    12601 ctatttccat tgggtaatat atcgacataa acaaataaag gaatcattct attgcatggc
    12661 cattaaatta gaaattaaaa atctttataa aatatttggt gagcatccac agcgagcgtt
    12721 caaatatatc gaacaaggac tttcaaaaga acaaattctg gaaaaaactg ggctatcact
    12781 tggcgtaaaa gacgccagtc tggccattga agaaggcgag atatttgtca tcatgggatt
    12841 atccggctcg ggtaaatcca caatggtacg ccttctcaat cgcctgattg aacccacccg
    12901 cgggcaagtg ctgattgatg gtgtggatat tgccaaaata tccgacgccg aactccgtga
    12961 ggtgcgcaga aaaaagattg cgatggtctt ccagtccttt gccttaatgc cgcatatgac
    13021 cgtgctggac aatactgcgt tcggtatgga attggccgga attaatgccg aagaacgccg
    13081 ggaaaaagcc cttgatgcac tgcgtcaggt cgggctggaa aattatgccc acagctaccc
    13141 ggatgaactc tctggcggga tgcgtcaacg tgtgggatta gcccgcgcgt tagcgattaa
    13201 tccggatata ttattaatgg acgaagcctt ctcggcgctc gatccattaa ttcgcaccga
    13261 gatgcaggat gagctggtaa aattacaggc gaaacatcag cgcaccattg tctttatttc
    13321 ccacgatctt gatgaagcca tgcgtattgg cgaccgaatt gccattatgc aaaatggtga
    13381 agtggtacag gtcggcacac cggatgaaat tctcaataat ccggcgaatg attatgtccg
    13441 taccttcttc cgtggcgttg atattagtca ggtgttcagt gcgaaagata ttgcccgccg
    13501 gacaccgaat ggcttaattc gtaaaactcc tggcttcggc ccacgttcgg cactgaaatt
    13561 attgcaggat gaagatcgcg aatatggcta cgttatcgaa cgcggtaata agtttgtcgg
    13621 cgcagtctcc atcgattcgc ttaaaaccgc gttaacgcag cagcaaggtc ttgatgcggc
    13681 gctgattgat gcgccgttag cagtcgatgc acaaacgcct cttagcgagt tgctctctca
    13741 tgtcggacag gcaccctgtg cggtgcccgt ggtcgacgag gaccaacagt atgtcggcat
    13801 catttcgaaa ggaatgctgc tgcgcgcttt agatcgtgag ggggtaaata atggctgatc
    13861 aaaataatcc gtgggatacc acgccagcgg cgggcagtgc cgcgcaatcc gcagacgcct
    13921 ggggtacacc gacgactgca ccggctgacg gcggtggtgc tgactggctg accagtacgc
    13981 ctgcgccaaa cgtcgagcat tttaatattc tcgatccgtt ccataaaacg ctgatcccgc
    14041 tcgacagttg ggtcactgaa gggatcgact gggttgttac ccatttccgt cccgtcttcc
    14101 agggcgtgcg cgttccggtt gattatatcc tcaacggttt ccagcaattg ctgctgggta
    14161 tgccagcgcc agtggcgatt atcgttttcg ctctcatcgc ctggcagatt tccggggtcg
    14221 gaatgggcgt ggcgacgctg gtctcgctga ttgccatcgg cgcaatcggt gcctggtcgc
    14281 aggcaatggt gactctggcg ctggtgttaa ctgccctgct gttctgtatc gtcatcggtt
    14341 tgccgttggg gatatggctg gcgagaagtc cgcgagcggc gaaaattatt cgtccactgc
    14401 ttgatgccat gcagaccacg ccagcgtttg tttatctggt gccaatcgtc atgctatttg
    14461 gtatcggtaa cgtgccgggc gtggtggtga cgatcatctt tgcgctgccg ccgattatcc
    14521 gtctgaccat tctggggatt aaccaggttc cggcggatct gattgaagcc tcgcgctcat
    14581 tcggtgccag cccgcgccag atgctgttca aagttcagtt accgctggcg atgccgacca
    14641 ttatggcggg cgttaaccag acgctgatgc tggccctttc tatggtggtc attgcctcga
    14701 tgattgccgt cggcgggctg ggtcagatgg tacttcgcgg tatcggtcgt ctggatatgg
    14761 ggcttgccac cgttggcggc gtcgggattg tgatcctcgc cattatcctc gatcgcctga
    14821 cgcaggccgt tgggcgcgac tcacgcagtc gcggcaaccg tcgctggtac accactggcc
    14881 ctgttggtct gctgacccgc ccattcatta agtaactctg cacttgcccg gtgacgccgg
    14941 gcattatcac cctgccaaaa aaaggaataa caatgcgaca tagcgtactt tttgcgacag
    15001 cgtttgccac gcttatctct acacaaactt ttgctgccga tctgccgggc aaaggcatta
    15061 ctgttaatcc agttcagagc accatcactg aagaaacctt ccagacgctg ctggtcagtc
    15121 gtgcgctgga gaaattaggt tataccgtca acaaacccag cgaagtggat tacaacgttg
    15181 gctacacctc gcttgcttcc ggcgatgcaa ccttcaccgc cgtgaactgg acgccactcc
    15241 atgacaacat gtacgaagct gccggtggcg ataagaaatt ttatcgtgaa ggggtatttg
    15301 ttaacggcgc agcacagggt tacctgatcg ataagaaaac cgccgaccaa tacaaaatca
    15361 ccaacatcgc acaactgaaa gatccgaaga tcgccaaact gttcgatacc aacggcgacg
    15421 gtaaagcgga tttaaccggt tgtaaccctg gctggggctg cgaaggtgcg atcaaccacc
    15481 agcttgccgc gtatgaactg accaacaccg tgacgcataa tcaggggaac tacgcagcga
    15541 tgatggccga caccatcagt cgctacaaag agggcaaacc ggtgttttat tacacctgga
    15601 cgccgtactg ggtgagtaat gagctgaagt cagggaaaga tgtggtctgg ttgcaggtgc
    15661 cgttctccgc actgccgggc gataaaaatg ccgataccaa actgccgaat ggcgcgaatt
    15721 acggcttccc ggtcagcacc atgcatatcg ttgccaacaa agcctggacc gagaaaaatc
    15781 cggcggcagc caaactgttt gccattatgc agttgccagt ggccgatatt aacgcccaga
    15841 acgccattat gcatgacggc aaagcctcag aaggcgatat tcagggccac gttgatggct
    15901 ggatcaaagc ccaccagcag cagttcgatg gctgggtgaa tgaggcgctg gcagcgcaga
    15961 agtaattttt attcgggcgg ataaggcgtt ttcgcctcat ccgccgctct gtacacaatg
    16021 cctgatgcga cgctgacgcg tcctatcatg cctacaaacc acatcgcaca tttcaacaag
    16081 ctatctttca tcccatattc atcaacatcc gctattattg atttccagct taatcatcac
    16141 ctgatgaaca aaaataatga ctaaacctaa tcatgagctt agcccggcgc tgatcgtgct
    16201 gatgtctatc gccaccggtc tggcggtagc cagtaactat tacgcccagc cattgctcga
    16261 caccatcgcg cgtaactttt ccctttccgc cagttcggca ggctttattg ttaccgccgc
    16321 gcagttgggc tatgccgcag gtctactgtt tcttgttccc ctcggtgata tgtttgaacg
    16381 ccgccgcctg attgtctcga tgaccttact ggcagcgggc ggtatgttaa tcactgccag
    16441 cagtcagtca ctggcgatga tgatcctcgg tacggcatta accggtttat tctcagtcgt
    16501 ggcacaaatt ctggttccgc tggcagcgac gctggcttca ccggataaac gcggcaaagt
    16561 ggttggcacc attatgagcg gtctgctgtt ggggatattg ctggcacgaa ccgttgccgg
    16621 gttactggca aacctcggcg gctggcgcac cgtcttttgg gttgcttcgg tgttaatggc
    16681 actgatggcg ctggcattat ggcgtggtct gccacaaatg aaatcagaaa cccacctcaa
    16741 ctacccacag ttgttgggtt ccgttttcag catgttcatc agcgataaga tcctgcgcac
    16801 ccgcgcgttg ctgggctgca tgacctttgc caacttcagc attctctgga cctcaatggc
    16861 ctttttgctt gccgctccac cttttaacta cagcgatggt gacattggtc tgtttgggct
    16921 tgcgggagct gccggagcgt tgggcgctcg tccggcgggc ggttttgccg acaagggcaa
    16981 atcgcaccac accacaactt tcggtctgct tctgctgtta ctttcatggc tggcaatctg
    17041 gtttggtcac acttccgtac tggcgttgat tatcggcatt ctggtgctgg acctcaccgt
    17101 acagggcgtg catatcacta accagacggt aatttatcga atacatcctg atgcgcgtaa
    17161 tcgcctgacc gcaggttaca tgaccagcta ctttattggc ggtgccaccg gttcgctaat
    17221 ttcagcctca gcctggcaac atggcggttg ggctggcgtt tgtctggctg gcgcgacgat
    17281 tgccctggtt aacttactgg tctggtggcg aggttttcat cgtcaggaag ccgcaaatta
    17341 agcaatcagc aaccgttttc gtggctttac acttataagg gtgttaagaa gcccatcagt
    17401 ctgataaggt taagatattc attcagtcta tttataatat taacaatcgt taagcgtaca
    17461 ctctatggaa agccctactc cacagcctgc tcctggttcg gcgaccttca tggaaggatg
    17521 caaagacagt ttaccgattg ttattagtta tattccggtg gcctttgcgt tcggtctgaa
    17581 tgcgacccgt ctgggattct ctcctctcga aagcgttttt ttctcctgca tcatttatgc
    17641 aggcgcgagc cagttcgtca ttaccgcgat gctggcagcc gggagtagtt tgtgggttgc
    17701 tgcactgacc gtcatggcaa tggatgttcg ccatgttttg tatggcccgt cactgcgtag
    17761 ccgtattatt cagcgtctgc aaaaatcgaa aaccgcccta tgggcgtttg gcctgacgga
    17821 tgaggttttt gccgccgcca ccgcaaaact ggtacgcaat aatcgccgct ggagcgagaa
    17881 ctggatgatc ggcattgcct tcagttcatg gtcatcgtgg gtatttggta cggtaatagg
    17941 ggcattctcc ggcagcggct tgctgcaagg ttatcccgcc gttgaagctg cattaggttt
    18001 tatgcttccg gcactcttta tgagtttcct gctcgcctct ttccagcgca aacaatctct
    18061 ttgcgttacc gcagctttag ttggtgccct tgcaggcgta acgctatttt ctattcccgt
    18121 cgccattctg gcaggcattg tctgtggctg cctcactgcg ttaatccagg cattctggca
    18181 aggagcgccc gatgagctat gaggttctgc tgcttgggtt actggttggc gcggcgaatt
    18241 attgcttccg ctatttgccg ctgcgcctgc gtgtgggtaa tgcccgccca accaaacgtg
    18301 gcgcggtagg tattttgctc gacaccattg gcatcgcctc gatatgcgct ctgctggttg
    18361 tctctaccgc accagaagtg atgcacgata cacgccgttt cgtgcccacg ctggtcggct
    18421 tcgcggtact gggtgccagt ttctataaaa cacgcagcat tatcatccca acactgctta
    18481 gtgcgctggc ctatgggctc gcctggaaag tgatggcgat tatataaccc acaagaatca
    18541 tttttctaaa acaatacatt tactttattt gtcactgtcg ttactatatc ggctgaaatt
    18601 aatgaggtca tacccaaatg gatagttcgt ttacgcccat tgaacaaatg ctaaaatttc
    18661 gcgccagccg ccacgaagat tttccttatc aggagatcct tctgactcgt ctttgcatgc
    18721 acatgcaaag caagctgctg gagaaccgca ataaaatgct gaaggctcag gggattaacg
    18781 agacgttgtt tatggcgttg attacgctgg agtctcagga aaaccacagt attcagcctt
    18841 ctgaattaag ttgtgctctt ggatcatccc gtaccaacgc gacgcgtatt gccgatgaac
    18901 tggaaaaacg cggttggatc gaacgtcgtg aaagcgataa cgatcgccgc tgcctgcatc
    18961 tgcaattaac ggaaaaaggt cacgagtttt tgcgcgaggt tttaccaccg cagcataact
    19021 gcctgcatca actctggtcc gcgctcagca caacagaaaa agatcagctc gagcaaatca
    19081 cccgcaaatt gctctcccgt ctcgaccaga tggaacaaga cggtgtggtt ctcgaagcga
    19141 tgagctaacg cgtcatctcg ctcaaaaatc cagatttata aaagaaaaaa tgaccggcca
    19201 gcatcgcaac atgctggcct ttttggcaag caggtcggct cagccgatga gttaagaaga
    19261 tcgtggagaa caatatgagc gcaaatgcgg agactcaaac cccgcagcaa ccggtaaaga
    19321 agagcggcaa acgtaagcgt ctgctcctcc ttctcacctt gctctttata attattgccg
    19381 tagcgatagg gatttattgg tttttggtac tgcgtcattt cgaagaaacc gatgacgcat
    19441 acgtggcagg gaatcagatt caaattatgt ctcaggtgtc tggcagcgtg acgaaagtct
    19501 gggccgataa caccgatttt gtaaaagaag gcgatgttct ggtcactctc gacccgacag
    19561 atgctcgcca ggcgtttgaa aaagccaaaa ctgcactggc ttccagcgtt cgccaaaccc
    19621 accagctgat gattaacagc aaacagttgc aggcgaatat tgaggtgcaa aaaatcgccc
    19681 tcgcgaaagc acaaagcgac tacaaccgcc gtgtgccgct gggcaatgcc aacctgattg
    19741 gtcgcgaaga gctgcaacac gcccgcgacg ccgtcaccag tgcccaggcg caactggacg
    19801 tcgctattca acaatacaat gccaatcagg cgatgattct ggggactaaa ctggaagatc
    19861 agcctgccgt gcaacaggct gccaccgaag tacgtaacgc ctagctggcg ctggagcgta
    19921 cccgtattgt cagtccgatg accggttatg tttcccgtcg tgccgtgcaa ccgggtgcgc
    19981 aaattagccc aacgacgccg ctgatggcgg tcgttccagc caccaatatg tgggtggatg
    20041 ccaactttaa agagacgcag attgccaata tgcgcatcgg tcagccggtc accatcacca
    20101 ccgatattta cggcgatgat gtgaaataca ccggtaaagt ggttggtctg gatatgggca
    20161 caggtagcgc gttctcactg cttccggcgc aaaatgcgac cggtaactgg atcaaagtcg
    20221 ttcagcgtct acctgtgcgt atcgaactgg accagaaaca gctggagcaa tacccgctgc
    20281 gcattggttt gtccacgctg gtgagcgtca ataccactaa ccgtgacggt caggtactgg
    20341 caaataaagt acgttcaaca ccggtcgcgg taagtaccgc gcgtgaaatc agcctggcac
    20401 ctgtcaataa actgatcgac gatatcgtaa aagctaacgc tggctaatcc agaggtgcgt
    20461 gtgatgcaac agcaaaaacc gctggaaggc gcgcaactgg tcattatgac gattgcgctg
    20521 tcactggcga cattcatgca ggtgctggac tccaccattg ctaacgtggc gatccccact
    20581 atcgccggga atctgggctc atcgctcagc cagggaacgt gggtaatcac ttctttcggg
    20641 gtggcgaatg ccatctcgat cccgcttacc ggctggctgg caaagcgcgt cggggaagtg
    20701 aaactgttcc tttggtccac catcgccttt gctattgcgt cgtgggcgtg tggtgtctcc
    20761 agcagcctga atatgctgat cttcttccgc gtgattcagg ggattgtcgc cgggccgttg
    20821 atcccgcttt cgcaaagtct attgctgaat aactacccgc cagccaaacg ctcgatcgcg
    20881 ctggcgttgt ggtcgatgac ggtgattgtc gcgccaattt gcggcccgat cctcggcggt
    20941 tatatcagcg ataattacca ctggggctgg atattcttca tcaacgtgcc gattggcgtg
    21001 gcggtggtgt tgatgacact gcaaactctg cgcggacgtg aaacccgcac cgaacggcgg
    21061 cggattgatg ccgtggggct ggcactgctg gttattggta tcggcagcct gcagattatg
    21121 ctcgaccgcg gtaaagagct ggactgtttt tcatcacagg aaattatcat tcttaccgtg
    21181 gtggcggtgg tggctatctg cttcctgatt gtctgggagc tgaccgacga taacccgata
    21241 gtcgatctgt cgttgtttaa gtcgcgcaac ttcaccatcg gctgcttgtg tatcagcctc
    21301 gcgtatatgc tctacttcgg cgctattgtt ctgctgccgc agttgttgca ggaggtctac
    21361 ggttacacgg cgacctgggc aggtttggcc tctgcgccgg tagggattat tccggtgatt
    21421 ctgtcgccga ttatcggccg cctcgcgcat aaactggata tgcggcggct ggtaaccttc
    21481 agctttatta tgtatgccgt ctgcttctac tggcgtgcct atacctttga accaggtatg
    21541 gattttggcg cgtcggcctg gccgcagttt atccaggggt ttgcggtggc ctgcttcttt
    21601 atgccgctga ccaccattac gctgtctggt ttgccaccgg aacgactggc ggcggcatcg
    21661 agcctctcta actttacgcg aacgctggcg gggtctatcg gcacgtcgat aaccacgacc
    21721 atgtggacca accgcgagtc gatgcaccat gcgcagttga ctgagtcggt aaacccgttc
    21781 aacccgaatg cccaggcgat gtacagtcaa ctggaagggc ttgggatgac gcaacagcag
    21841 gcatcaggct ggattgccca gcagatcacc aatcaggggc tgattatttc cgccaatgag
    21901 atcttctgga tgtcagccgg gatattcctc gtactgctgg ggctggtgtg gtttgctaaa
    21961 ccgccatttg gcgcaggtgg cggcggaggc ggtgcgcact aagtacaact aagccagttc
    22021 atttgaactg gcttttttca attaattgtg aagatagttt actgactaga tgtgcagttc
    22081 ctgcaacttc tctttcggca gtgccagttc ttcgttgctg ttgatgcgta cgtcacgttc
    22141 cagaatgcta cgcgcaatat cctgcgcttc ctgcaacgag tgcatctggt aagtgccaca
    22201 ctggtagacg ttcagttccg ggatctgatt ctgatcctgc actttcagca cgtcttccat
    22261 tgccgctttc caggcatcag caacacgctg ctcatctggc gtaccaatca gactcatata
    22321 aaaaccggtg cggcagccca ttggcgagat atcgataatc tctacaccat taccgttaag
    22381 atggttacgc ataaaaccag caaacaggtg ctccagggta tggatccctc tttctggcat
    22441 cacttctttg ttcggcacgc agaagcgcag atcgaacacg gtgattgcgt cgccatgcgg
    22501 ggtgttcatt gttttcgcca cccgaactgc aggcgcttcc atccgggtat gatcgactgt
    22561 gaagctatct aacaacggca tttagccacc tccggtaatt tttttaaaaa ttttctgaac
    22621 tctttcttcc caggcgagtc tgagtatatg aaagacgcgc atttgttatc atcatccctg
    22681 aattcagaga tgaaattttg gccactcacg agtggccttt ttcttttctg tcaggcgtgt
    22741 ttttccagcc acaccgcaaa cggttcggta tcagcggttt ccatttcctg ctgacggcgt
    22801 tcagatgcct cgcgctcggc tacaaaatcc tcttcccgca gaatttccag cggctcttca
    22861 cgcagcagat tacggtaggc ttctgcaaac gctttgcctg ttccgccaat accagtatca
    22921 atcatagacc ttaagatacg ggcagagaaa gtcagatcgg gattatcgaa gcaggcaacc
    22981 agttcatcac acactttctg atacgcttcg ccgccgttaa tgctatccag cgtttgcgcg
    23041 acgcgtttca gatcgcggaa cagatcttta cccacctgca gtaacgggaa ctgcgcggtt
    23101 tcgcagccga tacccagcgt caggcccggt ttgcgacctt caaggatcac ccggttccag
    23161 ttaacgcgcg tacaggcaag ttcgctactg ctcatttccg gtgcatcagc cagcgcacac
    23221 cagaccataa acaggtcgag gaatcgcacc tgctgttcat ctacaccaat cggcgagaac
    23281 gggttgatgt ccagcgaacg cacttcaata tattcaatgc cgccacgtaa cagcgcatca
    23341 gaaggtgact cgccgctgcg ggtaacgcgt tttggacgaa tcggcgcata cagttcgttt
    23401 tcaatctgca gcacgttgct attgatttgc agcctcttac cgtctttctc aataccaatc
    23461 ttcgcgtact cttccgatgg cgttttgatt gcctgtttaa ggcccgctac gtactcgtaa
    23521 agatcgttga aggtaatacc aagattgctt tgcgatttat tggtatagcc gagatcgctc
    23581 aaacgaagag aggtcgcata cggcaggtaa tacataccgc actcggtttt ctcaaacggc
    23641 agcgacgttg gttttccttg caggaaagaa gaacaaatcg ccggagatgc accaaacaga
    23701 taaggaatga cccaaccgaa acgatagtaa ttgcggataa cgcggaaata gcccgcagaa
    23761 attttctctt tggcatcagc gcccgagata tcaccgcact tcgcttgcca gaatgccatt
    23821 ggcaaagaga aattgtagtg cacgccggaa atggtttgca tcagcgcgcc gtagcgattt
    23881 ttcagccctt cacgatacag cgttttaaag cgtccggtgt tagaagtgcc gtactgtgcc
    23941 agttcgatgt cctgaccttc tgcgatgtag catggcatac ttaacggcca catccgctca
    24001 tcgcccatat tgcgcgccgt ataacgatgc agatcgcgca taaaggtcag catatgttca
    24061 atatcaccat ccactggtgt aatgaattcc agcaatgctt ccgcaaaatc ggtagtaatc
    24121 catttgtgcg tcagtgcgga acctaatgct tcaggatgac ctgttgttgc cagtgtgcca
    24181 tcagcattaa cacgcaaagt ttcgcgctcc agcccacgct gtatcccctt taacgcctga
    24241 ggatgttttt ccagccaggc cagcgcctgt gatacgtccg ggatcaaatt gacctcccgc
    24301 ctgtcaaaat cgttttaatt agcataactg taatggtgac catatgtgca ggcctacaat
    24361 tagtgccacc acatcatgcc ctgaacggtc gctgctgcaa ctgcaacata gcgtaacgct
    24421 ttaccaaggc acaaaaaaaa gattactggc ccccaggaga tgcgcatcca tcccgctaac
    24481 agacacagta aatcgccaac cacgggcatc cagcttaata atagcgtgac tgcaccatag
    24541 cgtttcagcc agccggtagc tttctcttgc cagcgcgatg ttttacgcaa tggaaagaaa
    24601 cgcccaagga taacgttagt taaccctcca aggctattac ccattgttgc tgttaaaact
    24661 aaaacccagg gatgactgat cccggaaagc aacattgcca ccagcacgac ttcggagttg
    24721 ccgggtaata gtgtagcgct gagaaaacta ctggcaaaca acgagaaaag cgataacgct
    24781 tcactcacag caagcgaaca tccacagcgt ccatgcctgc tgcacgggcc gcctgaatac
    24841 cgaaatcggc atcttcaaag accacacact gcgtcggttg cacgcccata cgctgcgcgc
    24901 acaacaaaaa tgtgtctggc gcgggtttat ggtgtttgac gtgatcagca gcgacgacgg
    24961 cgtcaaaata acggcgtaat cccaggtgcg ccagcaatgc ctcagcgatg gcgctttcac
    25021 tccccgttcc tacagccatt gggcgacgac catgccaact tttcaccact tcaacaagag
    25081 gaagcggctc gacactatcc agcagcatac ttcttactgc ttctgttttt tcacgcgcta
    25141 acgcatgcgg gtcgagatcg gcctgattca gctcaataat tgcctgagca atacgccagg
    25201 tgggcgatcc attaagcgca atcatcgcct gaatatcgta ctgaagaccg tagtgcccta
    25261 atacttcgcg ccacgcttta cggtgcgtag gctccgtatc caggattgtg ccatccatat
    25321 caaaaattaa acctgcataa cgctcgtaca tggtcttctc gcaagtcgaa aaatatgacg
    25381 ctactttagc gtaattgctt gattttgtcg ctgatggcga aggagaagaa aattcaggaa
    25441 atggataaag tagttattta gaaatgagat atttttgaag gaaatttttt ggagaa
//
LOCUS       DADLSA010000047        24961 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.047, whole genome shotgun sequence.
ACCESSION   DADLSA010000047 DADLSA010000000
VERSION     DADLSA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.047"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(572..1348)
                     /gene="sseB"
                     /locus_tag="JN691_001928"
     CDS             complement(572..1348)
                     /gene="sseB"
                     /locus_tag="JN691_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAY7243383.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGAAAQGE
                     AVVEDSVLDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSGQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1490..2773)
                     /gene="pepB"
                     /locus_tag="JN691_001929"
     CDS             complement(1490..2773)
                     /gene="pepB"
                     /locus_tag="JN691_001929"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAY7243384.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2832..3032)
                     /gene="iscX"
                     /locus_tag="JN691_001930"
     CDS             complement(2832..3032)
                     /gene="iscX"
                     /locus_tag="JN691_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAY7243385.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3044..3379)
                     /gene="fdx"
                     /locus_tag="JN691_001931"
     CDS             complement(3044..3379)
                     /gene="fdx"
                     /locus_tag="JN691_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAY7243386.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3381..5231)
                     /gene="hscA"
                     /locus_tag="JN691_001932"
     CDS             complement(3381..5231)
                     /gene="hscA"
                     /locus_tag="JN691_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAY7243387.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKTLAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5248..5763)
                     /gene="hscB"
                     /locus_tag="JN691_001933"
     CDS             complement(5248..5763)
                     /gene="hscB"
                     /locus_tag="JN691_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAY7243388.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5859..6182)
                     /gene="iscA"
                     /locus_tag="JN691_001934"
     CDS             complement(5859..6182)
                     /gene="iscA"
                     /locus_tag="JN691_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAY7243389.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6199..6585)
                     /gene="iscU"
                     /locus_tag="JN691_001935"
     CDS             complement(6199..6585)
                     /gene="iscU"
                     /locus_tag="JN691_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAY7243390.1"
                     /translation="MAYSEKVIDHCENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6613..7827)
                     /gene="iscS"
                     /locus_tag="JN691_001936"
     CDS             complement(6613..7827)
                     /gene="iscS"
                     /locus_tag="JN691_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY7243391.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(7939..8427)
                     /gene="iscR"
                     /locus_tag="JN691_001937"
     CDS             complement(7939..8427)
                     /gene="iscR"
                     /locus_tag="JN691_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAY7243392.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8697..9437)
                     /gene="trmJ"
                     /locus_tag="JN691_001938"
     CDS             complement(8697..9437)
                     /gene="trmJ"
                     /locus_tag="JN691_001938"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAY7243393.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9556..10359
                     /gene="suhB"
                     /locus_tag="JN691_001939"
     CDS             9556..10359
                     /gene="suhB"
                     /locus_tag="JN691_001939"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAY7243394.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            10504..11358
                     /locus_tag="JN691_001940"
     CDS             10504..11358
                     /locus_tag="JN691_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7243395.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     APWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            11549..12829
                     /gene="csiE"
                     /locus_tag="JN691_001941"
     CDS             11549..12829
                     /gene="csiE"
                     /locus_tag="JN691_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAY7243396.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12821..13960)
                     /locus_tag="JN691_001942"
     CDS             complement(12821..13960)
                     /locus_tag="JN691_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAY7243397.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGCEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYPYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14120..15010)
                     /gene="hcaR"
                     /locus_tag="JN691_001943"
     CDS             complement(14120..15010)
                     /gene="hcaR"
                     /locus_tag="JN691_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HAY7243398.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSTEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            15146..16507
                     /locus_tag="JN691_001944"
     CDS             15146..16507
                     /locus_tag="JN691_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HAY7243399.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWVLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16504..17022
                     /locus_tag="JN691_001945"
     CDS             16504..17022
                     /locus_tag="JN691_001945"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HAY7243400.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17022..17342
                     /locus_tag="JN691_001946"
     CDS             17022..17342
                     /locus_tag="JN691_001946"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HAY7243401.1"
                     /translation="MNRIYACPVEDVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            17339..18151
                     /gene="hcaB"
                     /locus_tag="JN691_001947"
     CDS             17339..18151
                     /gene="hcaB"
                     /locus_tag="JN691_001947"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(cis-5,6-dihydroxycyclohexa-1,
                     3-dien-1-yl)propanoate dehydrogenase"
                     /protein_id="HAY7243402.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTCSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALEQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18161..19363
                     /locus_tag="JN691_001948"
     CDS             18161..19363
                     /locus_tag="JN691_001948"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HAY7243403.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIAIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            19460..19882
                     /locus_tag="JN691_001949"
     CDS             19460..19882
                     /locus_tag="JN691_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY7243404.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(19930..20802)
                     /locus_tag="JN691_001950"
     CDS             complement(19930..20802)
                     /locus_tag="JN691_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY7243405.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGNKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(20814..21896)
                     /locus_tag="JN691_001951"
     CDS             complement(20814..21896)
                     /locus_tag="JN691_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY7243406.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAKGRGAKRIIGVDMLPER
                     LAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTADWGR
                     VVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAITHRF
                     SLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(21929..22927)
                     /locus_tag="JN691_001952"
     CDS             complement(21929..22927)
                     /locus_tag="JN691_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7243407.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVLID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIK
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(22952..24463)
                     /locus_tag="JN691_001953"
     CDS             complement(22952..24463)
                     /locus_tag="JN691_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7243408.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     KGASKSTLIRKLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(24486..>24961)
                     /locus_tag="JN691_001954"
     CDS             complement(24486..>24961)
                     /locus_tag="JN691_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY7243409.1"
                     /translation="AFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKL
                     IISTPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAV
                     VDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
BASE COUNT         5881 a         6980 c         6229 g         5871 t
ORIGIN      
        1 tcagacaaat ataaacaaat cctgctcaaa attaaaaatt ctaaccggta aaagatatta
       61 cttaaacatg taaatttact ttcctttaaa aaacaaaaaa ccgccaaaat caggcggtta
      121 tttgttgctg gtccggttcg cggcctttcc agcaggttgt attaccgtag taatgcaagc
      181 gcgtctcagc ggagacaata ctcgccagta actctctttt tgtcaagcaa aagagagtta
      241 ttattgttct gttagtgtat tatccactgc ggccctttcc gccgtctcgc aagcgggcgc
      301 tggcttaagg aaaggatgtt ccgcgaccgt tagagcaagc gcgtctcagc gcttgctatc
      361 gcggcaatat cgccagtggt gctgtcgtga tgcggtcttc acatggaccg cacaatgaag
      421 atacggtgct tctgtatcgt acttattgtt tctggtgcgc tgttttccca agtaaataat
      481 aaccggagtc tctccggcga caatttactg gtggttaaca accttcagag cagcaagtaa
      541 gcccgaatgc cgccctttag ggcggcatat tttagattat ccggttttgt ttaaagtcac
      601 gcaaaaaacc accccagcga cgttcataga atggcgcaat atgttcggta ataaagtggc
      661 taattccttt ttcccctttt ttcacctgac aaatatcgat tggttcatcg ccaggtaatg
      721 tatcggtcgc tacacttccc gtcgcctgaa taatttcttc gatatcacca tcggcttcaa
      781 tgccaataag taaattaggc tgtgcctctt cgttctcttt aattgaacaa ataaaagcac
      841 gcttcaccgg cttaatggtt ttaaataagg tggtgagtga atcaatcatt tgtgctggcg
      901 gctctgcaac ttccgataat atcagcgatt caccgccttc caggatttcc tggccgctca
      961 gcggatttcc ctcttcacca atcaacaaac tgatttcacg cggcataaat tctttaccgg
     1021 tcggcagttt ggcattaagg aagagcgttt cgccaagggt catctcaaac aatgtgcgaa
     1081 cgggcattac gacaaatgcc tgttcgtctt caaccgcctg ttgaagcgct tctaacgagg
     1141 tgaaaaaagg aatgacgctg gtgccgtctt ctttttccca gtgctgtaaa tcaagcacac
     1201 tatcttcaac cacagcctcg ccctgcgccg ccgcaccagg cacccagacg gtggattcca
     1261 gtagagtacg gaaaaaggcc gggcggtgcg ccggttcggt tgctgctttt tccagcaggt
     1321 cttcaagttc gttttttgtt tcggacataa gaaccacaat tcattcaacg ttcggggcgc
     1381 aaatgcctga tgcgctacgc ttatcaggcc tacaagggat tcgcaatttg ttgaatttgc
     1441 agaatttgta ggccggataa ggcgtttacg ccgcatccgg catattagtt tacgccgtta
     1501 acagattagc tatcgtgcgc acaccaagtc ccgtagcgcc cgcagaccac tgttcaaccg
     1561 gcgctttacg gtaagtcgcc gagcagtcga tatgcagcca gccttgctga tagttctcaa
     1621 caaagtgcga caggaagccc gccgccgtgc tcgcgcctgc cggatacgcc gcgcttccgg
     1681 tattgttcag ttcggcaaag ttagacggca gctggctgcg gtggaattcc gccagcggca
     1741 gacgccagaa tggttcgttc tcttgtgagg cactcgccag taaacgaccg gcaagcgcat
     1801 cgtcaaaact gaacagcgcg tgataatcat tacccagcgc agttttcgcc gccccggtga
     1861 gggtcgccgc atcaatgatc atttccggtt tctgcgcact ggcatcaatc agaccatcgg
     1921 caagcaccag acgcccttcg gcatcagtgt tcatcacttc aactttttta ccgttgcgat
     1981 aggtgatgat atcgcccagc ttgaacgcat tgccgctaat caggttatcc gcacagcaga
     2041 ggaacagctt cacgcgcttg ttcagtccgc gcgtaatggc aaatgccagc gccccggtaa
     2101 ccgttgccgc gccgcccatg tccgacttca tcgagtccat aaacgcagtc tgtttgatgc
     2161 tgtagccgcc ggagtcaaaa gtgatacctt tacctaccag gcacgcgtac accggtgctt
     2221 ctttatcgcc agttgggttg taatccagcg ccagcaatac cggagaacgt tctgaaccgc
     2281 gcccgacagt gtgcagcccc atataacctt gctcacgcag atcttcgcct ttggtgatcc
     2341 gataagtcac acgatcgccc gcgacgttgc tgatcagatc aacagcacgc tgtgccagtt
     2401 gcgatggtcc caattcttct gccggtgcgt tgatggtgtc acgcacccag tcgatgatca
     2461 tcaggcggtt atccagttcc tggcgctggg catcgtccag atccggccac tccactttac
     2521 gcgtgccttt cgggcctttg taaccttgcc agaatgccca gcagcgatcc gcatcccagc
     2581 cttcaccgct taactgaaca tgcttgatgc ccagaccgtc aatcttgcgg gccgcacgct
     2641 ggatcagccc cagatcgtct gccccgttca aatgcagggt aatgccgtca ttattaatgc
     2701 tgtaagttgc tttttctccc cagcgcgcgt cggcaggttg ggtagagagg gtaatcttca
     2761 tcgcttctgt cattttagtt atccttattc gtttaacagc aaaacgggct acccccaggc
     2821 agcccgttca ttcactcggc ttcgtccagc cagactaaca aaatcgcttc gaggattttc
     2881 tcgttggatg cctgcgggtc gtcgtcgaaa tcttccagat cgcaaatcca ctgatgcata
     2941 tcggtgaatc gaaccgtttt cggatcaaga tcgggatacg catcgtacag tgcttcgcca
     3001 atttcgcggc tatcggtcca cttaagtccc atactaacct ctgttaatgc tcacgcgcat
     3061 ggttgatagt gtaacgcggg atttcgacca ctaaatcttc gtcggtgacg cgcgcctggc
     3121 aacttaaacg gctttccggc tccagtcccc aggctttgtc cagcatgtcg tcttcctgct
     3181 ctgagctttc cggcagtgag tcaaaacctt cacgaacgat gcagtggcag gtggtgcaag
     3241 cacaggattt ttcacaggcg tgttcaatct cgataccgtt acgcagcgct gcgtcgagaa
     3301 tggtttcacc gctattagct tccagaacag cgccatcagg gcagaggtcc tgatgaggca
     3361 aaataacaat ctttggcata ttaaacctcg tccacggaat ggcctttcag cgcacgacga
     3421 accgactggt ccatgcggcg agcggcgaaa tcctgggttt gtttgtctac gtttttaatc
     3481 gcttgttcga tggcgtcaac atcatcgccc tgcgccactt cactcaggtg agcggcagca
     3541 ttgtcaatga cctgacgctc tgcggcgctt aacagcgcgg catcagcagc cagcgcgccg
     3601 tgcagacttt ccagcacacg cgccgcttct actttttgtt ctgccagcat acgggctttt
     3661 acgtcctgct cggcatagct cattgagtct ttgatcatcg aagcgatttc gctatcggtc
     3721 agaccgtaag acggtttgac ctgaatagac gcctcaacgc cggtggattt ctccatcgcc
     3781 gtcacgctca aaagaccgtc ggcatcgacc tggaacgtca cgcgaatatg cgcaccgcca
     3841 gccggtagcg ccggaatacc acgcagcgca aaacgcgcca gtgagcggca gtcctgcacc
     3901 agttcgcgct caccctgcat tacatggata gacatcgccg tctgaccatc tttaaaggtg
     3961 gtgaaatcct gagcgcgggc caccggaata gtggtattac gcggaatcac tttctccacc
     4021 aggccgccca tcgtttcgag gcccagcgac agtgggatca catcaagcag cagcatttcg
     4081 ctgtctggct tgttacccac cagaatatcc gcctgaatcg ccgcgccaat agcgacgact
     4141 ttatccgggt cgatggaagt cagcggtgga cgaccgaaaa attcgcctac ccgttcacgc
     4201 accagcggca cgcgagtaga accgcccacc atcaccactt ccagcacttc gtcagcttct
     4261 acacccgcgt ctttcagcgc gcgacgacaa gccagtaagg ttcgttttac cagtggcgcg
     4321 atcagttcat tgaattgttc acggctgatt tcgccctgcc agcccgcaac gttaacggtc
     4381 acggagtccg catcgctcag cgcgattttg gctgcaatgg cggcatccag cagttcacgc
     4441 tgaacgcggt tatcgctacg atcaggaatg tccgcctgct cgcgaatgta atccgccagc
     4501 agatggtcga aatcatcgcc gccgagcgcg gaatcaccgc cggttgccag cacttcaaac
     4561 acgccgcgac ttaagcgcag aatggaaata tcaaacgtcc cgccaccgag gtcataaaca
     4621 gcgatcacgc cttcctgacc ggaatccagc ccgtaggcga tagccgcagc ggtcggttcg
     4681 ttaagtaagc gcagaacatg caaccccgcc agacgcgccg cgtctttggt gccctgacgc
     4741 tgggcatcgt caaagtacgc cggaacggtg ataactacac catccagctc gcctgccagg
     4801 gcttcagttg cccgcgccgc cagtgttttg aggatgtccg cagaaacgcg caccgggttc
     4861 agtagccccg ccgccgtttc aatcatcggc aggccgtttt cgctggcctg gaattgataa
     4921 ggcagatgcg gatagcgttg ctggatatca gccagcgagc gccccatcag gcgtttaaca
     4981 gaactaatgg tgttggcggt gtcaagcgct gcgttagtac gcgcgtcata acccaccgaa
     5041 tgcccttgct gttgatagtg aacaacagat ggcagcaggt gacggccttc atggtcggct
     5101 aacgtttcgg cctgaccgct gcgcactgtc gccaccagcg agttggttgt gcccaggtca
     5161 ataccagccg ccagacgacg ctgatgcggc gcagcactca aaccaggttc actaatttgt
     5221 aataaggcca tgtttagctt ccagaaatta aaaatcgagc agtttttctt cgagttgttc
     5281 ggcactgctc cgcagtttat cgagaaaacg cagcttacgc acggtatccg ccgccgcgtc
     5341 ccacgcctcg ttgtctaact gttcaaccat caactgatgg cgggtatcaa acatcttttt
     5401 cacacgtttg ataaagcttt ccagccgcgc ttcatctttc gcctgttcga tctcgtccag
     5461 ctcttcgcgc agctccaact gttccatcag gaacgcggtg tcgcgcacag tatgctgctc
     5521 gctggcgaga tcaaaaccgt gtaaagaaag caaatattcc gcgcgcatta acggatgacg
     5581 cagcgtttgc caggcctggt taatggttgc agattgctgt acggcggcga gttgttccgc
     5641 ctggcttccg ctggcgaatt tatcaggatg atactgacgt tgtagatcct gaaaacgcag
     5701 gctcagtgcc tgggtatcga gttgataacg ggcaggcaag ccaaagaggg tgaagtaatc
     5761 cataacattc tcagggctgc gatccgccgc gataacggcg aatcaggtaa aacaaacccc
     5821 acgcgcaggc gaccacggtg gggttatcgg tatgcgcatc aaacgtggaa gctttcgccg
     5881 caaccacact catctttaac gttcgggttg gtgaatttaa acccttcatt caggccttct
     5941 tttacgaagt ccagctgcgt accgtccaga aattgcaggc ttttgccatc gaccacgact
     6001 ttcacgcctt tgtcttcaaa cacgatgtct tccggcgtcg gttcgtcaac aaattccagt
     6061 acataagcca tacctgaaca tccggaggtt ctcacgccca gacgcaggcc aaaccctttg
     6121 ccgcggttag ccagaaaggt atttactcgc gctgctgcac tgtcgctcag tgtaatcgac
     6181 ataaccaaac ctcaactctt attttgcttc acgtttgctt ttatagtccg caatggcggc
     6241 tttgatcgcg tcttctgcca gaatagaaca gtgaattttc accggcggca gttcaagttc
     6301 ttcagcaata tcggtgtttt tgatcgcctg cgcttcgtcg agagacttcc ctttcaccca
     6361 ttcggtgacc agggagctgg aagcgatagc ggaaccgcag ccgtaagttt taaaacgcgc
     6421 gtcttcaatg ataccttcat cgttgacttt aatctgcaac ttcattacgt cgccacaggc
     6481 cggtgcaccc accatgccgc tgccgacgtt ctcgtcgttg ttgtcaaagg aacccacgtt
     6541 acgcggattc tcgcaatggt cgataacttt ttcgctgtaa gccattataa attctcctga
     6601 ttccgatacc gattaatgat gagcccattc gatgctgttc agatccacgc cctgcttgta
     6661 catttcccac agcggagaaa ggtcacgcag acgaccgatg gatttacgaa ctaactcgat
     6721 ggtgtagtcg atctcttctt cagtagtaaa acgacctaaa gagaaacgga tagagctatg
     6781 tgccagctcg tcgttcagcc ccagcgcgcg cagcacgtag gacggttcga ggcttgctga
     6841 cgtacaggcg gaacctgaag aaactgcgag gtctttcagc gccataatca gcgactcacc
     6901 ttcaacgtag ttgaagctga cgttgagaat gttcggcgca ccgtgttcca ggtcaccgtt
     6961 caggtaaact tcttcgatat ctttgatgcc gttccacaga cggttacgca ggccgcgcag
     7021 acgttccatc tcggtcgcca tctcttcttt tgcgatgcga taggcttcgc ccattccgac
     7081 gatctggtga acaggcagag tgccggaacg cataccgcgc tcgtgaccgc cgccgtgcat
     7141 ttgcgcttcg atgcgtacac gcggtttacg acgcacatac agcgcaccga tacctttcgg
     7201 gccatagatt ttgtgaccgg agaaagacat caggtcaact ttcaactggc tcaggtcgat
     7261 tggcagtttg cccacgctct gggttgcatc aacgtgatag ataatgccac gagcacggca
     7321 catttcgccg atagccgcga tatcctgcac cacgccgatt tcgttattta cgtgcatgat
     7381 ggaaacaagg atggtgtcgt cacgcatcgc cgcttcgagt tctttcaggt cgataatacc
     7441 gttacgctgc ggtgccaggt aggtgacttc gaaaccttcg cgctccagct gacggcaagt
     7501 atccagtacc gctttgtgtt cggttttgct ggtgatgatg tgcttgcctt ttttctgata
     7561 aaagttggct gcacctttga tcgccaggtt gtcagattcg gttgcaccag aggtaaagac
     7621 gatttcacgc ggatcagcgc cgaccagatc ggcaatctga ttacgggcga tatctaccgc
     7681 ttcttcagcc tgccagccga aacggtgaga acgagaggcc gggttaccaa aggttccgtc
     7741 catcgtcatg aactgcatca ttttctcggc aacacgcggg tccaccggcg tggttgcgga
     7801 gtagtcgaga taaatcggta atttcattgc tctataaact ccgtacatca ctcaatgcaa
     7861 ggaatcaggc taccggctgg atgtacgacc gtgtttacgg agtatttagc actccggcct
     7921 gattctgaat tctttttatt aagcgcgtaa cttaacgtcg atcgcgtctt gtgtgcgggt
     7981 gcgtggcgcg tcgtgagtat gctgacgacc agacacatcc agcacttcct ggttattaac
     8041 cagttcgcct aaagtaatgt tgttgagaaa accggtgaga cggtcgctca aatcacgcca
     8101 cagcgcgtgg gtcaggcatt tatcgccgcc ctggcagccg cctttaccct gacaacgggt
     8161 ggcatctaca gattcgtcaa cggcgctaat cacttcgcca acggcgatgc tgctggcatc
     8221 tttgcctaac agataaccac cgcctggtcc acgtacgctg gaaaccagac catttttacg
     8281 cagacgggaa aacagttgtt ccagataaga aagggaaatt ccctgacgtt cggaaatatc
     8341 agccaacggt accgggcccg cttcagagtt gagcgcaacg tcaagcattg cggtcacggc
     8401 atagcgccct ttagatgtca gtctcatgtc ttacttcacc tcaaactcgc ccctgcccgg
     8461 ggttttttat tgtaaaagtg ggggtattgc atagcagggt caagtctgac attcccgagt
     8521 aaattggtca actatttact tgactgattt agtcgggtat ttaaccttca gtgccatttt
     8581 tttatcgtgg cgtttgtacc tgttgtcgga tgcggtgtga acaccttatc cgacctacga
     8641 ttctgtacat tgtaggcctg ataagacgcg ttaagcgtcg catcaggcaa ccactgttat
     8701 tcggccttgt tacctttatt ctgctgctca atagaagcca gaatcccgcg caggatattc
     8761 aattcctggc tttccgggcg cgcacgggta aacagacggc gcaatttatt catcacctgc
     8821 cccggatggt tttcacggat aaaaccggtt gccagcagcg tttgttccag atgaccataa
     8881 aaacgctcca gatcatcgac cagcggatac ggcgtctctt catgctcgac ctgctcgcca
     8941 ttttcctgag ttgccagcca ggccatacgc acttcatagg caataacctg aaccgccatt
     9001 gccaggttca gcgaactgta ttccgggttt gccgcaatcg cgacatgata atggcatttc
     9061 tgcaactctt cattggtcaa gccgacgcgc tcacgaccaa acaccagcgc caccggggta
     9121 tttgccgctt cagcgacgct tttcagaccg cattcgcgcg ggtcgagcat cggccacggc
     9181 agcgtgcggg agcgtgcgct ggtgcccacc accaggctac aaccggctaa cgcttcgtcg
     9241 agcgtatcga cgatgtgagc attaccaatc acatcgctgg cccctgctgc cagggcaatc
     9301 gcctgggagt cgggtttcac cagtggatta accagccaca gattggttaa tcccattgtt
     9361 ttcatggcac gggcaacaga acccatattg ccggtgtgtg acgtctccac cagcacaatt
     9421 cgaatatttt gcagcattgt ctttcttcat ctaaagatta ttcacgcatc ttatcataaa
     9481 acgaagacag atgccgatct cgctgctata ctctgcgccg ttttcccgtt ctttaacatc
     9541 cagtgagaga gaccgatgca tccgatgctg aacatcgccg tgcgcgcagc gcgcaaggcg
     9601 ggtaatttaa ttgccaaaaa ctatgaaacc ccggacgctg tagaagcgag ccagaaaggc
     9661 agtaacgatt tcgtgaccaa cgtagataaa gctgccgaag cggtgattat cgacacgatt
     9721 cgtaaatctt acccacagca caccatcatc accgaagaaa gcggtgaact tgaaggtact
     9781 gatcaggatg ttcaatgggt tatcgatcca ctggatggta ctaccaactt tatcaaacgt
     9841 ctgccgcact tcgcggtatc tatcgccgtt cgtatcaaag gccgcaccga agttgctgtg
     9901 gtatacgatc ctatgcgtaa cgaactgttc accgccactc gcggtcaggg cgcacagctg
     9961 aacggctacc gtctgcgcgg tagcaccgct cgcgatctcg acggtaccat tctggcgacc
    10021 ggcttcccgt tcaaagcaaa acagtacgcc actacctaca tcaacatcgt cggcaagctg
    10081 ttcaacgaat gtgcagactt ccgtcgtacc ggttctgcgg cgctggatct ggcttacgtc
    10141 gctgcgggtc gtgttgacgg tttctttgaa atcggtctgc gtccgtggga tttcgcggca
    10201 ggcgagctgc tggttcgtga agcgggcggc atcgtcagcg acttcaccgg tggtcataac
    10261 tatatgctga ccggtaacat cgttgctggt aacccgcgtg ttgtgaaagc catactggcg
    10321 aacatgcgtg acgagttaag cgacgctctg aagcgttaat gactcaggcg ggtgatatca
    10381 ctcacccgcc ctcgcctttc aggcgctatt ccgaaatact tcctcaccgc tttactttct
    10441 ttcgtcactc tcccaccatc tttccccgat taatggatga taaagaagtc gcaaggaaag
    10501 gttatggcac tgccagtgaa caaacgcgtt cccaaaattc tgtttattct ctttgttgtt
    10561 gccttctgcg tttatttagt gccgcgcgtt gccatcaact tcttctatta tcctgacgat
    10621 aaaatttacg gtcccgcccc ctggtcggcg gaatccgtcg aatttacggc taaggacggt
    10681 actcgtctgc aaggctggtt tattccttct tcgacgggcc ctgctgacaa cgccatcgca
    10741 accatcattc atgctcacgg caatgccgga aatatgtccg cccactggcc gctggtcagt
    10801 tggttacctg agcgtaattt caacgttttt atgtttgatt atcgcgggtt tggtaaatca
    10861 aaaggcacgc cgtcccaggc cggattgctg gacgatacgc aaagcgccat caatgtggtg
    10921 cgccatcgca gtgatgtaaa cccacaacgg ctggtgctgt tcgggcagag tattggcggg
    10981 gcgaatattc ttgctgttat tggtcagggt gatcgtgaag gcatacgtgc ggtgatcctc
    11041 gactccacat ttgcctctta tgcaaccatc gccaaccaga tgatccccgg cagtggctac
    11101 ttacttgatg agagttacag cggcgaaaat tacatcgcca gcgtcagccc gatcccgctt
    11161 ttactcattc acggtaaagc cgatcacgtt atcccatggc agcacagcga aaagttgtat
    11221 agcctggcaa aagagccaaa acggttgatc ctaatcccgg atggcgaaca cattgatgct
    11281 ttttccgatc gtcacggcaa tgtttatcgc gaacagatgg tggactttat ccttagcgcg
    11341 ttgaatccgc agaactaacc catgatcgct agcacgataa tcattcacaa aaccacctta
    11401 agacatgcta atccactggt cagaacagtt taaaatgaga aaaattctgt gacgcttgcc
    11461 aacatttctg atgattagca ttcccttcgc catttccctg agcaaacttt agctattctt
    11521 atcaattatg cttatgggag atctacagat gatgcctacg cttgctccac catctgtcct
    11581 ttcggctccc cagcgccgct gccagatctt gctgacgctc tttcagccgg ggttgaccgc
    11641 caccatggca accttcagcg agcttaatgg tgtggatgat gatattgcca gtcttgatat
    11701 cagcgaaaca ggacgggaga tcctgcgcta tcatcaactc acactgacga ctggttatga
    11761 cggtagctac cgggttgaag gtacagtgct taaccaacgt ttgtgtttat ttcactggct
    11821 acgacgtggt ttccgtctgt gtccgtcatt tattaccagc caattcaccc ccgccctgaa
    11881 gagtgaactg aagcggcgcg gaattgcgcg taacttttac gacgatacca atctacaagc
    11941 gttagtgaat ctctgctccc gacggctgca aaaacgcttt gaatcgcgcg atattcattt
    12001 cctgtgtctg tatctgcaat attgtttgct gcagcaccac gctggaatta cgcctcagtt
    12061 taatccgctc caacgtcgct gggcggaatc ctgccttgaa tttcaggtag cgcaggaaat
    12121 tggacgccac tggcagcgtc gggcgctcca gcatgtaccg cctgatgagc cactgtttat
    12181 ggcattactt ttttccatgt tgcgggttcc cgatccactg cgggatgcgc atcagcggga
    12241 cagacaattg cgtcagtcta tcaaacgtct ggtaaaccat tttcgtgagc tgggaaatgt
    12301 tcgtttttat gatgaacagg ggttatgtga tcagctttat atccacctcg cccaggcgtt
    12361 aaatcgcagt ttgtttgcca tcggtattga taataccctg ccggaagagt tcgccagact
    12421 gtatccacgc ctggtacgca ccacccgcgc ggcgctggcc ggatttgaaa gtgaatacgg
    12481 cgtccatctt tctgatgagg aaagtggtct ggtcgcggtg attttcggtg cctggctaat
    12541 gcaggaaaac gatctgcatg aaaaacagat tattctactg accgggaatg atagcgagcg
    12601 agaagcgcag attgagcagc agttacgcga actaacgtta ctgccgctca acattaagca
    12661 tatgtcggta aaggcatttt tgcagacagg cgctccgcgt ggcgcggcac tgattattgc
    12721 gccttatacc atgccgttac cgctcttttc accaccgctg atctatacgg acctgacgct
    12781 gacaacacat caacaggagc agatccgcaa aatgctcgaa tcagcatgag ggaacaactt
    12841 tcgggcgcaa aaacattgcc ggaagcgcca ccagcgccat tacccagaac acgccgtggc
    12901 ccagatatgg atacaggaaa ccggcgaaaa cggtcatgat agcgatactg ccgcccatcg
    12961 cgacggcaga gtaaaccgcc tgtaaacgga tgacttcgca accctggcga gcagcaatat
    13021 aacgcatggc ggccaggtgg cagaccgtga aggtgccgca atgcagaatt tgcaccacta
    13081 tcaaccacgg caacgccgta gttgctccca taatgcccca gcgcactacg ccgcaaatcg
    13141 ccgagatcaa cagcatatcg cgagcactac aacggcggaa aagtttatta ctcagcgcaa
    13201 agataatgac ttccgccacc acgcccagcg accacaaata ccccaccgcc gaggccgagt
    13261 agccagctgc ctgccagtaa atggcgctaa aaccgtaata ggccgcatgt gccccctgca
    13321 ataaacaaac gcaggccaga aagcgccagt tctggcgaac cagcgccaac cacgcagacc
    13381 aaccggtgct ctcctgctgg cggcttgccc cttgtggctg aatcgtcgga cggatgagaa
    13441 agccgagcag catggatgcc acgcccaacg tcaacagcac gaggatcacc cgataatcaa
    13501 acatagtgac cagtttgccc gtcagcgccg agccaatgac aaacgccacc gagccccaca
    13561 gtcgcacttt gccgtaatca agcgggaact gcttttgcca cgtattcgcc agtgcatcgg
    13621 tcaacggtac cagcggtgag aaaaagaggt taaagccaat catcaccagc atcagccacg
    13681 ctacgtgcgc ccccgcccag aaggcgacag caaagagaag tggcagcagt gccagcacgc
    13741 gcaaggcgga aatcaggcgg gaaggatcgc tgacgcgggg cgcgatgagc aaactcccga
    13801 gaaaacgggc aaccagacct gcccccaata acaggccgat ggtttctggc gttaaaccaa
    13861 tccctttaag ccagacgctc cagaaaggta aaaaaatgcc gtaactaaaa aagtatgtga
    13921 aatagccgag cgccaaccag cgcgtggatt gcaaaaccat gagtccctcc cgtcaggagg
    13981 cgttagtctg gggttaatcg aatgttggtg caagttgaaa aataagaata ttcgagcctg
    14041 cttcccgatg tctgtaacgc cagttcacaa gcactaaaaa actgcgatcc gcgacgcatg
    14101 ttgcaaaaat aatgcgtaat tatgccgtta cgcttgccaa acgttcctgc acaatggcga
    14161 tgaaatcgcg taatgccggc ttcatctccc ctttcttcca cgccatcagt aaagcaatgg
    14221 aaggcacatt tccggcaatg ggacgaaaaa caacctgccc ggtattaaaa ttattcatat
    14281 aaccgggtat caaagtgacg cccagcccca tgcccaccag attcatggtc accagaatat
    14341 tcgttgccac ctggacgata tttggctggc tgttttcttg cgcaaaccac gctttaacga
    14401 tcggcgcaag cgaaccggaa tacgccggat cggtactgac gaaattcacg ccatccagtt
    14461 gcgcggcggt gatctctttt tcatgtgcta aagggtgatc aaccggtaac accaccacta
    14521 atggttcgtc aaaaagctcc agataatcaa tctccgtgct ataaacagga tggcgcatta
    14581 agccgacgtc gagttcacca cggcgaattt tttcctcctg ttgcgtggtg attaaactca
    14641 ccagctcaat caaggtgtct ggctgtctga gacgaaacat cggtaatact tttggcaata
    14701 aattcacttc cgccgatggc acaaagccaa tggttaattg tctgtcttcc tgaacaattt
    14761 tccgcgcccg taatttggca ttttccgctt gttcgaggat tgccagcgcg tcctggagaa
    14821 aacattctcc cgccgccgtc aacgcgactt tgcgcttatc cctcaccagt aacggaacac
    14881 cgacacagtt ttcaagatcg cggatctggc tgcttaacga aggctgtgag gtatgcagtt
    14941 tttccgccgc acgggtaaag ttcagtgcct gcgccactgc gacgaaatag cgtaaatgcc
    15001 gtagttccat caccttcccc ttgttatcga aaaaacgtct cagctggtag aaattaacta
    15061 tttcacatat tagcaaccaa ccagcaacat ccttatggca caaaaataga aggtcaatac
    15121 atcttatctt tcaggattaa aaaatatgac cacaccctca gatttgaaca tttaccaact
    15181 gattgacacc caaaacggtc gggtcactcc gcgtatttat accgacccgg acatttacca
    15241 actggagctt gagcgtattt tcggtcgttg ctggctattt ctcgcccacg aaagccagat
    15301 cccaaaaccc ggtgatttct ttaacaccta catgggagaa gatgcagttg tcgtggtgcg
    15361 ccagaaagac ggcagcatca aggcgtttct caaccaatgc cgccaccggg ccatgcgtgt
    15421 gagttatgca gattgtggca acactcgcgc ctttacctgc ccgtatcacg gctggtctta
    15481 tggcattaac ggcgagttga tcgatgtacc gctggaacct cgcgcctacc cacaagggtt
    15541 gtgtaaatcc cactgggtgc taaacgaagt tccttgtgtg gagagttata aaggactgat
    15601 ttttggcaac tgggatacca gcgcaccggg cctgcatgat tacctgggtg acattgcctg
    15661 gtatctggat ggcatgctgg atcgtcgcga aggcggcacc gaaattgtcg gcggcgtaca
    15721 aaagtgggtg atcaactgta actggaaatt cccggcagag cagttcgcca gtgaccagta
    15781 tcatgctctg ttcagccatg cttctgccgt tcaggtatta ggggcgaaag atgatggcag
    15841 cgataagcgc ctcggtgatg gacaaaccgc ccgcccggtg tgggaaaccg ccaaagatgc
    15901 gctgcaattt ggtcaggacg gtcacggtag cggtttcttc tttactgaaa aaccggatgc
    15961 taatgtctgg gtcgatggcg cagtttcaag ctattaccgc gaaacctatg ccgaagcaga
    16021 acaacgttta ggtgaagttc gcgccctgcg cctggcgggt cataacaata tattccccac
    16081 gctttcatgg ctaaacggca ctgccacact ccgcgtctgg catccgcgcg gccctgatca
    16141 agttgaagtg tgggcgttct gtattactga caaagccgcc tccgatgaag ttaaagccgc
    16201 ttttgaaaac agcgccactc gtgcttttgg tcctgctggt tttctcgagc aggatgactc
    16261 ggagaactgg tgtgaaatcc agaaattgct taaaggccac cgcgcccgca acagcaaact
    16321 gtgtctggaa atggggcttg gtcaggaaaa gcgccgcgac gacggcattc ctggcattac
    16381 taactatatc ttttcagaaa ctgccgctcg tggaatgtac caacgctggg ccgatcttct
    16441 gagtagcgaa agctggcagg aagtgctcga taaaaccgcc gcttaccagc aggaggtgat
    16501 gaaatgagtg cgcaagtttc actggagtta catcaccgca ttagccagtt tctctttcac
    16561 gaagccagtc tgctggatga ctggaaattt cgtgactggt tggcacagct cgacgaagag
    16621 attcgttaca ccatgcgcac cacggttaac gcgcaaacac gcgaccgtcg caaaggcgtc
    16681 cagccgccga cgacctggat tttcaatgac accaaagacc agctggagcg gcgaatcgcc
    16741 cgtctggaaa cgggcatggc ctgggcagaa gagccgccgt cacgcactcg tcacttaatc
    16801 agcaactgcc aggtaagcga aaccgacatc ccaaacgtat ttgccgtgag ggtgaattat
    16861 ctactttatc gggcacaaaa agagcgcgat gaaacattct atgttggaac gcgtttcgac
    16921 aaagttcgcc gtctggaaga tgacaactgg cgcttgctgg aacgggatat cgtcctagat
    16981 caagcggtga tcacttccca taacctgagt gtactgttct gatgaatcga atttatgcgt
    17041 gtcccgttga ggatgtgccg gagggtgagg ctctccggat agatacctcg cccgtcatcg
    17101 ccttgttcaa cgtgggcggc gagttttatg ccattaacga tcgttgcagc catggtaatg
    17161 cgtcgatgtc agaagggtat ctggaagatg acgccacggt ggagtgcccg ctacacgccg
    17221 ccagtttttg cctgaaaacg gggaaagcgt tatgcctgcc cgccaccgat ccgctcacca
    17281 cttatccagt acacgttgaa ggtggtgaca ttttcatcga cttaccggag gcgcagccat
    17341 gagcgatctg cataacgagt ccatttttat taccggcggc ggatcgggat tagggctggc
    17401 gctggtcgag cgatttatcg aagaaggcgc gcaggttgcc acgctggaac tgtcggcggc
    17461 aaaagtcgcc agtctgcgtc agcgatttgg cgaacatatt ctggcggtgg aaggtaacgt
    17521 gacctgttat gccgattatc aacgcgcggt cgatcagatc ctgacttgtt ccggcaagct
    17581 ggattgtttt atcggcaatg caggcatctg ggatcacaat gcctcactgg ttaatactcc
    17641 cgcagagacg ctcgaaaccg gcttccacga gctgtttaac gtcaacgtac tcggttacct
    17701 gctgggcgca aaagcctgcg ctccggcgtt aatcgccagt gaaggcagca tgattttcac
    17761 actgtcaaat gccgcctggt atcctggcgg cggtggcccg ctgtacaccg ccagtaaaca
    17821 tgccgcaacc ggacttattc gccaactggc ttatgaactg gcaccgaaag tgcgggtgaa
    17881 tggcgtcggc ccgtgtggta tggccagcga cctgcgcggc ccacaggcgc tcgagcaaag
    17941 tgaaacctcg ataatgcagt ctctgacgcc ggagaaaatt gccgccattt taccgctgca
    18001 atttttcccg caaccggcgg attttacggg gccgtatgtg atgttggcat cgcggcgcaa
    18061 taatcgcgca ttaagcggtg tgatgatcaa cgctgatgcg ggtttagcga ttcgcggcat
    18121 tcgccacgta gcggctgggc tggatcttta aggaagcacg atgaaagaaa aaacgatcat
    18181 tattgtcggt ggcgggcaag cggcagcaat ggctgcggcc tcgctacgcc agcaagggtt
    18241 caccggtgag ctgcatctgt tttccgatga gcgacatctt ccttatgaac gacctccact
    18301 ctcgaaatcc atgttgctgg aagattcccc gcagttacag caggtgttac ccgctaactg
    18361 gtggcaggaa aacaatgttc atctgcattc cggtgtaacc atcaaaacgc tgggtcgcga
    18421 cacacgagag ttagtgttaa ccaacggcga aagctggcac tgggatcagc tttttatagc
    18481 aaccggcgcg gcagctcgac cgctgccgtt gcttgatgca ctgggagaac gctgctttac
    18541 cctacgccat gccggtgatg ccgccagact gcgagaagtt ctgcagcccg aacggtcagt
    18601 cgtgattgtc ggtgccggaa ctattggtct ggaactggct gccagcgcca cgcagcgcag
    18661 atgtaaggtg acagtgattg aactggcggc aaccgtcatg ggccgtaatg caccaccgcc
    18721 cgtgcaacgc tatcttttac agcgccatca gcaggctggt gtgcgcattc tgctcaataa
    18781 tgccattgaa catgtggtcg atggtgaaaa agtagaactg acgctgcaaa gtggggaaac
    18841 gcttcaggct gatgtggtga tttacggtat tggtatcagc gccaacgagc aactggctcg
    18901 cgaggccaac cttgatactg ccaatggcat tgccattgat gaggcttgcc gcacctgcga
    18961 tcccgcgatc tttgccggtg gcgatgtggc aatcactcgt cttgataatg gtgcactaca
    19021 ccgctgcgaa agctgggaaa acgccaataa ccaggcgcaa attgccgctg ccgcaatgtt
    19081 agggctaccg ctaccgctac tgccgccgcc gtggttctgg agcgatcagt acagtgataa
    19141 cttacagttt attggcgata tgcgtggcga tgactggctt tgtcgtggca acccggaaac
    19201 tcagaaggcg atttggttta atctgcaaaa cggcgtgctt atcggtgcgg tcacgctgaa
    19261 tcaggggcgt gagattcgcc caattcgcaa atggatccag agcggcaaaa cgtttgatgc
    19321 gaaactgctg atagatgaga acatcgcgct taaatcactg taaccaggat aattagcgaa
    19381 tatctcaatg cctggggcgt ggcgaggtgc aagagtgtgt attacgttta aatcacatta
    19441 tcttgcaaag ggattggtta tgaacacact acgttatttt gattttggag ctgcccgccc
    19501 cgttttgtta ttaattgccc gtatcgccgt ggtcttaatt ttcattattt ttggttttcc
    19561 caaaatgatg ggctttgacg gtacggtcca atatatggcc tcgttgggcg cgccaatgcc
    19621 gatgctggca gcgattattg cggtagttat ggaagtgccc gccgcgatat taatcgtgct
    19681 tggctttttc acccgtccgc tggcggtgct gtttattttc tacacgctgg gtacggcggt
    19741 gattggtcac cattactggg atatgaccgg cgatgcggtt gggccaaata tgattaattt
    19801 ctggaagaat gtcagtatcg ctggcgcgtt cttgctattg gcaattaccg ggccgggggc
    19861 aatttctctc gatcggcgtt aggaaagatg ccggatgcgg cgtgaacgcc ttatccggca
    19921 ttttataaat tacagccact ctacccgcaa cgacatctct gaggttgttg attcccctgg
    19981 tgctagcgca atgagatcac ccccttccgg gcgatgatga tcatccggcg catgactcat
    20041 cggttccaga cagaagaaat caaacgcata tcctttatca aacgcagggt cggaaacaaa
    20101 gatgaaataa cacggtgcag gtggcgtcgt ttccatgatg atggcataac cttcctgcgg
    20161 ctgctcgatg cgggcctgac cattccatcc ggcaaaacca ttgttcaccc actgacgcgg
    20221 caacggcgcg agctggttaa aatctagttc ctgcggtagc tgctcgcaaa actcacccgc
    20281 cagccactgc tcccgttcca gccagtaacc gctcgcctgc gcctgaatcc gcgtttgtgg
    20341 tgacaacgga aaataaggat gccagccggt accaaatggc agcgtctctg ccccttgatt
    20401 ggtgacagag agcgtcaccg tcagcgtatc cgccgttaaa tgaaacgcct gacttacccg
    20461 atagtgatag acaccgctgc gatgttcata caccagacac aaactatcat cgctgtgcga
    20521 gacacattgc cactcgccca gccagccatc gccgtgtaga tagtgcgcat cccactcaac
    20581 attcggttgc agttgatact cacgcccctg ccagacaaaa cggttgccgc tcacccggtt
    20641 tgcaaatggc accagcggaa aacatgaggc atcggttgcc acaccgcttt tgttaccagg
    20701 acgtaagagc ggcgtcgtat cgcgccagaa gccttcgatt acgccgccct ggtcagaaac
    20761 gtccagcttt agcgacccgt gggataaggt atagatggtc atccgctaac tccttaatcc
    20821 gggaagttaa tcacaacttt cccgcatttg ccgctcgcca tcagcacata ggcatcacct
    20881 gcctgttcta gcgagaagcg atgggtaatg gcgttacgcg gccacagctt ccagtcagtc
    20941 aaatcgtggg cgcatttttc catatggaac agactggtca cccaggagcc gataatccgc
    21001 cgttgatggt gcatcagatc agcgctgacc tcgaattcca cttttccggt ttcaccaatg
    21061 taaaccaccc gcccccagtc agcagtggat tgcagtgcca gcaagcgacc tgcggcatta
    21121 ccggaacaat cgagcgcaac atccgcgcca ccgtgggtaa gttcagcaat tatctgcggc
    21181 agaccttcgg tggttgctaa atagccgtga tccatcaccc ctaactgttt tgccatcgcc
    21241 agacgttccg gcagcatatc aacgccgatg atccgttttg caccgcgacc tttcgccatc
    21301 atgccgaccg gccccagacc gaccaccagc acgttatcgc tgccggaaac ttcgccgcgc
    21361 aaaatccctt cataggccgt gccaacgccg caactgataa acgcaccatc ttcgtagctc
    21421 agcgcatccg gcaggaggat cagatctttt tcttccgcca gcagatattc ggcatgaccg
    21481 ccgtcacgct gccagccgta agccgctttt cctttgccag tacaagagat aggaaagccg
    21541 cggcggcagt tcgggcaaaa accacagcca gaaatgtgat acaccagcac gcggtcgccc
    21601 tctttaaaat ggcggcagcc ttgccccatc gccacaatct gcccgcacgg ttcatgaccg
    21661 ttgataaatc cctggtataa cggtttatcg ggtgccgccg ccgtggcacg gtgttgatga
    21721 tagatatagt ggacatcgct tccgcaaatc ccggaggatt tcattttgat cagtacctgg
    21781 ttaatccccg gcgtcggcac cgcaacttcc cgcagatcga cggttgaatt tcctggtaaa
    21841 taagctgcca gcatcgtttt cataaatccc tcattaacaa tacgattaat tttcatccct
    21901 gccgcacccg cgccaggggc gttaaggttt agcgtttcgc tttactgctt cgctgggtca
    21961 gcaagatatt cgccagcacc gccaccacga tgatgacgcc gcgtaccacc tgctggaaaa
    22021 aggagttaat accgagcagc accagaccgt taccgattag cgtaatcacc agcacaccaa
    22081 gcaatgtacc gaacagggaa ccgcgaccgc cggaaagtgc cgtaccgccg accacgaccg
    22141 cggcgatgac gtcaaactcc agaccgtttg cggcacctgc gttaccggaa ccgaggcgtg
    22201 ccgccaacaa aatgccggtc accgccgcta ataatcccga aagggtaaag ataagaatgc
    22261 gcactcgacg aactttaata ccgcacaact gcgccgccgt ggcattaccg ccaacggcaa
    22321 aaaccgagcg cccgaaggcg gtttttcggc taatgaacac aaacagcgca aacaacacca
    22381 tcatgatcag cgcggatact ggcacaccga gaaattgtcc gcccagccag tccagcacct
    22441 cgttttcgtc aatcagcact ggcagtgcgt tggtcataaa cagccccatt ccgcgcaggg
    22501 cgctccacag ccccagtgtg gcaacgaaac ttggtacgtt aaacacgccg cgcagcaccc
    22561 cggcgagcgt tcccatcagc gcgcctaaca gcaacaccag cagacaagca atcgccaacg
    22621 gaacttcaaa ttgcagcaaa aatgccaggc acaccgagac aaaagccacc atcggcccaa
    22681 cgctgacatc aatttcaccg gagataataa tcagcgtcat cgcccaggcg gcaatcccaa
    22741 tggtggcggc atcgcgcagc acgttcatct ggttattcaa tgagataaag ccaggcgcgt
    22801 tcagggagaa gaccagataa agaatggcaa tcaccaccag caaaccgatc tcattaatat
    22861 ggcgactgac aaattgtttg agcgagacgc tcttgccctg cggcaatggt aatgacgaag
    22921 cagacatctt caggttcctc ggtaaaattc atcagtgcac agacagaatg gcggacatca
    22981 gctcatccac attgaccggc gagtgaaact cctgcgagaa cgtgccgtgc tgtaataaca
    23041 ggatgcggtc acacaccagc ggcaactcct ccacttcact ggagataaac accacgcttt
    23101 ttccttcggc agccagctca cggacaatac ggtaaatctg ctgtttggct tcgatatcga
    23161 cgccgcgcgt tggctcgtcg agcaacaaaa tctggctggc ggcatagacc caacgaccga
    23221 tcaccacttt ttgctgattg ccaccagaaa gcgtgccgat gggtgtttcg ctactggcag
    23281 ccttgaccgt catccgctgc atcacctctt cggtcaggcg gcggatggtg gaccattgca
    23341 gcacaccgtt ggtgctgatt ttttgccgat tggtcagcac tgtattttcg tcaacgccca
    23401 gccagggaat gatccccgct tctttgcggt tttctggcgt atagccaatg ccgcgtttca
    23461 gcatgtcgcc gtaatcgggg cgcatgattt tctcgccgtt gataacaatt tcgccctgtt
    23521 catacgtctc cagcccaaca atcgccttca gcaattcact gcgccctgcc cccagcaggc
    23581 cagcaatgcc aagcacttcg ccacgacgta gcgtaaagct gatatcctcc agcttgggct
    23641 tatggcgtaa cgcacggact tccagtacgg cctgatccac aatttcctga ggggcaaccg
    23701 gcgcaatatc aacgtgatca cgccctagca ttagcgacac aatatgatgc gtggaggtgt
    23761 tttcgagcat cacatcgccc gccacctgac catcgcgcat aacggtggca caggaggcaa
    23821 tgcggcggat ttcttccatc cggtggctga cataaatcac cgccacgccc agtgccgaca
    23881 tctttttcac cgcactgatc accagttcaa cttctgcact ggcaagcgag ctggtgggtt
    23941 catcaagaat gaccacgcgc ggctcgccct tcatcacccg tgcaatttcc accagttgct
    24001 tttgcgccgg gcttagcgtt gaaacaagtt gttcaggact aacgtcaacg cccagcgcct
    24061 gtaagcaacg ttgggcatcc tgcgccattt gcaggtaatc aatcatgccg ttgcggcgag
    24121 gccattgacc aaggcagagg ttttctgcca ccgtcagccc ttccaccaga cttaattcct
    24181 gataaaccgc acgaaccccc agttcagcgg cacggcgagt caacgtagct tcgtcacctt
    24241 ccagtcgcgt ctcgccaatc cagatatcac cgctatccgg gcgttcgctg ccggtaagct
    24301 ttcgaatgag agtcgatttg ctcgcgcctt ttttacctaa cagcgcacga acttcgcctt
    24361 tattgagcgt gaagttaacg ttatccagcg caacgacgcc ggggtaacgc ttatttcctg
    24421 ccaccacttt tgctaccggg actgcctctg ttgccgtgaa catagggacc tctgcgaatc
    24481 agcgattagg gcagaccatc aacgtgcgtt gccagccact gtttaccatc ttccgttttg
    24541 gtatagagat cgattggcac ctgaatcact ttttcaccgt cggcttgttt attgataacc
    24601 ttcaatgttt gcgcgaaaac agcattgccc attttcttac cggaaatatc cactaccgct
    24661 ttcagcacct gattgttttc cagctcctga gcaatttcgg ttgtcatatc cgaaccgaaa
    24721 acagcaattt ttccggcctg attttgatta cgtaccgctt ttaccgcgcc gagtgtcgca
    24781 ccgcccgact cccccataat ggcgttgaga tccggcgtgg agataatcag tttttcacca
    24841 acggaaatcg ctttatctaa aacagtccct tcctgattag cgacaatttg cgcgccggga
    24901 acgcgggatt ttaatacttc ttcaaatcct ttacgtcgct gcacacaaac ttcaaaggct
    24961 t
//
LOCUS       DADLSA010000048        24589 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.048, whole genome shotgun sequence.
ACCESSION   DADLSA010000048 DADLSA010000000
VERSION     DADLSA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.048"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(58..1083)
                     /gene="tdh"
                     /locus_tag="JN691_001955"
     CDS             complement(58..1083)
                     /gene="tdh"
                     /locus_tag="JN691_001955"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAY7243410.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1093..2289)
                     /gene="kbl"
                     /locus_tag="JN691_001956"
     CDS             complement(1093..2289)
                     /gene="kbl"
                     /locus_tag="JN691_001956"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAY7243411.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2564..3421)
                     /gene="yibB"
                     /locus_tag="JN691_001957"
     CDS             complement(2564..3421)
                     /gene="yibB"
                     /locus_tag="JN691_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HAY7243412.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3725..4657
                     /gene="rfaD"
                     /locus_tag="JN691_001958"
     CDS             3725..4657
                     /gene="rfaD"
                     /locus_tag="JN691_001958"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAY7243413.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4667..5713
                     /gene="rfaF"
                     /locus_tag="JN691_001959"
     CDS             4667..5713
                     /gene="rfaF"
                     /locus_tag="JN691_001959"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAY7243414.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5717..6709
                     /gene="rfaC"
                     /locus_tag="JN691_001960"
     CDS             5717..6709
                     /gene="rfaC"
                     /locus_tag="JN691_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAY7243415.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGVAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPRLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6706..7914
                     /gene="rfaL"
                     /locus_tag="JN691_001961"
     CDS             6706..7914
                     /gene="rfaL"
                     /locus_tag="JN691_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HAY7243416.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(7950..9092)
                     /locus_tag="JN691_001962"
     CDS             complement(7950..9092)
                     /locus_tag="JN691_001962"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HAY7243417.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9101..10114)
                     /locus_tag="JN691_001963"
     CDS             complement(9101..10114)
                     /locus_tag="JN691_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAY7243418.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILENNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKELPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDEAIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10139..10846)
                     /gene="rfaY"
                     /locus_tag="JN691_001964"
     CDS             complement(10139..10846)
                     /gene="rfaY"
                     /locus_tag="JN691_001964"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HAY7243419.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAAKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10872..11879)
                     /gene="waaO"
                     /locus_tag="JN691_001965"
                     /gene_synonym="rfaI"
     CDS             complement(10872..11879)
                     /gene="waaO"
                     /locus_tag="JN691_001965"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HAY7243420.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNLRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(11922..12719)
                     /gene="rfaP"
                     /locus_tag="JN691_001966"
     CDS             complement(11922..12719)
                     /gene="rfaP"
                     /locus_tag="JN691_001966"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAY7243421.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12712..13836)
                     /locus_tag="JN691_001967"
     CDS             complement(12712..13836)
                     /locus_tag="JN691_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY7243422.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQSKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13833..14891)
                     /gene="rfaQ"
                     /locus_tag="JN691_001968"
     CDS             complement(13833..14891)
                     /gene="rfaQ"
                     /locus_tag="JN691_001968"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAY7243423.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYLDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQDGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            15304..16581
                     /gene="waaA"
                     /locus_tag="JN691_001969"
     CDS             15304..16581
                     /gene="waaA"
                     /locus_tag="JN691_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAY7243424.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            16589..17068
                     /gene="coaD"
                     /locus_tag="JN691_001970"
     CDS             16589..17068
                     /gene="coaD"
                     /locus_tag="JN691_001970"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAY7243425.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(17107..17916)
                     /gene="mutM"
                     /locus_tag="JN691_001971"
     CDS             complement(17107..17916)
                     /gene="mutM"
                     /locus_tag="JN691_001971"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAY7243426.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLLLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(18014..18181)
                     /gene="rpmG"
                     /locus_tag="JN691_001972"
     CDS             complement(18014..18181)
                     /gene="rpmG"
                     /locus_tag="JN691_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY7243427.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(18202..18438)
                     /gene="rpmB"
                     /locus_tag="JN691_001973"
     CDS             complement(18202..18438)
                     /gene="rpmB"
                     /locus_tag="JN691_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAY7243428.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(18655..19323)
                     /locus_tag="JN691_001974"
     CDS             complement(18655..19323)
                     /locus_tag="JN691_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAY7243429.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            19495..20715
                     /gene="coaBC"
                     /locus_tag="JN691_001975"
     CDS             19495..20715
                     /gene="coaBC"
                     /locus_tag="JN691_001975"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAY7243430.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            20693..21151
                     /gene="dut"
                     /locus_tag="JN691_001976"
     CDS             20693..21151
                     /gene="dut"
                     /locus_tag="JN691_001976"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAY7243431.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     QGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            21258..21854
                     /gene="slmA"
                     /locus_tag="JN691_001977"
     CDS             21258..21854
                     /gene="slmA"
                     /locus_tag="JN691_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAY7243432.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(21891..22532)
                     /gene="pyrE"
                     /locus_tag="JN691_001978"
     CDS             complement(21891..22532)
                     /gene="pyrE"
                     /locus_tag="JN691_001978"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAY7243433.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(22598..23314)
                     /gene="rph"
                     /locus_tag="JN691_001979"
     CDS             complement(22598..23314)
                     /gene="rph"
                     /locus_tag="JN691_001979"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAY7243434.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            23441..24304
                     /locus_tag="JN691_001980"
     CDS             23441..24304
                     /locus_tag="JN691_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAY7243435.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEIMQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
BASE COUNT         6556 a         5963 c         5461 g         6609 t
ORIGIN      
        1 acataattaa cagattatcc tcagataaaa gggctggaat accagccctt gttcgtgtta
       61 atcccagctc agaataactt tcccggactg gcccgaacgc atagcgtcaa agcccttctg
      121 gaaatcatcg atagagaaac gatgggtaat gatcggcgag agatcgaggc cagactgaat
      181 cagcgccgcc atcttgtacc aggtttcaaa catctcacga ccgtaaatgc ctttaatgaa
      241 caagccttta aagatcactt tggtccagtc gatagacata tcagacggtg gaatacccag
      301 catcgcaata cgaccgccgt ggttcatggt atcgagcatg gtacgaaacg ctggcggcgc
      361 gccggacatt tccagaccga catcaaagcc ttcggtcatg cccagttcag ccatcacatc
      421 attaaggttt tctttcgcga cgttaaccgc acgggtgata cccattttgc gcgccagctc
      481 aaggcggtat tcgttaacat cggtgatcac cacattgcgt gcaccaacgt gtttcgccac
      541 cgccgcagcc ataataccta tcggacctgc accagaaacc agcacatctt cgcccaccag
      601 atcgaacgac agcgcggtat gcacggcgtt accgaaggga tcaaaaatgg aagccaggtc
      661 gtcggagata ttgtcgggga ttttgaaagc gttgaacgcc ggaatcacca gatattcggc
      721 gaagcagccc ggacggttaa caccgacacc aatggtgttg cggcacagat gggtacgacc
      781 gccacgacag ttgcgacagt gaccgcaggt gatatggcct tcgccagaaa cgcgatcgcc
      841 gattttgaag cctttcactt cctgaccaat acctaccact tcaccgacat attcatggcc
      901 cacgaccatc ggcaccggga tggttttttg cgaccactca tcccagttat agatgtgaac
      961 gtcagtcccg cagatggctg ttttacggat tttaatcagc agatcgttat gcccgagttc
     1021 cggtacaggg acgtcggtca tccagatgcc ctcttccgct ttcagtttgg ataacgcttt
     1081 catctcacat cctcaggcga taacgcccag ttgtttacca atacgcgtga atgcttctac
     1141 tgcacgcgta atttgctcag gggtatgcgc cgcagacatc tgggtacgaa tacgcgcctg
     1201 acctttcgga acgaccggat agaagaaacc ggtgacgtaa atgccctctt tttgcagctc
     1261 tcgggcaaat ttctgcgcca ctaccgcatc accaagcatg accggaataa tggcgtgatc
     1321 ggctcccgcc agggtaaagc ccgccgccga catttgctca cggaactgac gcgcgttcgc
     1381 ccacagacgg tcacgcaact cgctacccgc ttcaaccatc tccagcactt tgatggaagc
     1441 ggcgacaatc gctggtgcca gcgagttgga gaacaggtac ggacgagaac gctggcgcag
     1501 ccactcaacc acttctttgc gcgccgcggt ataaccacca gaagccccgc ccagcgcttt
     1561 accgagcgtg ccggtgatga tgtcgacacg ccccatcaca tcgcagtatt catgggaacc
     1621 acgaccattt tcaccgacaa aaccgaccgc gtgggagtcg tctaccatca ccagggcatc
     1681 gtatttatct gccagatcgc aaacgccctt cagattggca atcacgccgt ccatcgagaa
     1741 cacaccatcg gtggcaatca gcacatgacg cgcaccggct tcacgcgctt ctttcagacg
     1801 agcttcaagc tcctgcatat cgttgttggc atagcgatag cgtttagctt tacacagacg
     1861 cacaccatca ataatagacg cgtggttcag tgcgtcggag ataatggcgt cttccgcgcc
     1921 cagcagcgtt tcaaacaggc caccgttagc atcaaagcag gaagagtaga gaatcgcatc
     1981 ttccatcccc aggaatgccg ccagtttttg ttcaagctct ttatggctgt cctgagtgcc
     2041 gcaaataaaa cgcaccgaag ccatgccgaa accgtgagaa tccattcccg cctttgctgc
     2101 cgcaatcaga tccggatgat tcgccagccc gagatagttg ttggcacaaa agttaatgac
     2161 gtggcttcca tcagccacgg tgatatctgc ttgctgcgca gacgtaataa tgcgctcttc
     2221 tttaaacaac ccttccgccc gtgcggtttc cagatcgttg gttaactgct gataaaattc
     2281 tccacgcatt gcgattctcc agacagggca aattccagca catattaccc aaacttatag
     2341 gtcgcgacga gatgatgcgt taacacttct gcaaaattca ggataaataa cgtgtatctt
     2401 gatgtctctc aacacctaca gatagtagaa aaactgtaca gttttggctg agcataatga
     2461 tgagtgattt tactgcattg atataaaaag aaaaaatgat gccaaagcat catttttcct
     2521 atctcagttg gcatcaaaac gctacccact aatggaggga aacctatttt cttgaaagga
     2581 aaattctcag cgcctgcatt tttgccccta aagtgttact cctgaagcag cgaaacaaat
     2641 cgaaccattt tcctctcccc agatagttta ggttgaagag atcggaacgt ttgtaatagc
     2701 acatgacaaa aatgccctgg tcatcgtcca caatgttatt gtttagtgtt attttttgac
     2761 tttccagaac taatttatag aattctttcc atttatgctg tgaaccaaca attgcgcccc
     2821 caataatata tacatgattg ccaatcatga aatcaaaagc ctgttgttga gatgtaacag
     2881 taagcccttt ttttatagtg aaaagatgca tcttattttc atcaaatggg aaatcccaaa
     2941 ttttcaatcc tcgagttaca tttggcttac ggcaatagcc aaaatctatc cacgcaacaa
     3001 gaggcgtttt cactaaaccc atgttgatag ctttatttac aaaataggcc ttaaggttac
     3061 ataccaaaac atactctggt gaccagtact ctgggttttt taattgtcga ggctcaagtc
     3121 tatttgtaaa cgactcatct ttttgaattt tttcgatacg acttctgata tatctaaatt
     3181 tcttttttat atcgataaca ataaccgttg ttggttttcc gttgcgaatc gcttcaatcc
     3241 ttggtttcaa atcgggagaa gtgaaaataa tcatctcatt ttcaagtgcg gccagacgct
     3301 caaagtaact aaaataaaca tcaactgaac gagctagttt ttcacggaac cctttattag
     3361 cagtccaatc ccctctacca atgtcaaaat atgcagtgat aattgtggtg gatgatttca
     3421 ttactaataa gaccattaat ataaaaaaag aattatatca taaatattaa cagaatgtat
     3481 actgtatatt atccattcaa atgattccgt tatttatcat acatcggaat attgatacta
     3541 aagcactatt cacatgcaaa accaacatcc gccatgaagg actagctaaa acccaaacta
     3601 gtttgttgca attagcatcc ttgcacctct atgtaaaggg ctgaagggat tcggatgtga
     3661 tggtatgatt acagacattc gtgtctgaga ttgtctctga ctccataatt cgaaggttac
     3721 agttatgatc atcgttaccg gcggcgcggg ctttatcggc agcaacatcg ttaaggcctt
     3781 gaatgataaa ggcatcaccg atattctggt ggtggacaac ctgaaagacg gcaccaagtt
     3841 tgtgaacctg gtggatctgg atatcgcgga ctatatggat aaggaagact tcctgatcca
     3901 gattatggct ggcgaagagt tcggtgatgt cgaagcgatt ttccacgaag gcgcatgctc
     3961 ttccaccacc gagtgggatg gcaagtatat gatggataac aactatcaat actccaaaga
     4021 gctgctgcac tactgtctgg agcgcgaaat cccgttcctg tatgcctctt ccgcagccac
     4081 ctacggcgga cgcacctccg actttattga atcccgcgag tacgaaaaac cgttgaatgt
     4141 ctacggttac tcaaaattcc tgtttgatga atatgttcgt caaatcctgc cagaagcgaa
     4201 ctcgcagatt gttggcttcc gctatttcaa cgtttatgga ccgcgtgaag gccataaagg
     4261 cagcatggcg agcgtcgctt tccatctcaa cacccagctt aataacggtg aatcaccgaa
     4321 gctgttcgaa ggtagcgaga acttcaaacg tgacttcgtc tatgtgggcg acgtggcaga
     4381 tgtaaacctg tggttcctgg aaaatggcgt ttccggtatc ttcaacctcg gcaccggtcg
     4441 tgcagaatcc ttccaggcag tagcagatgc tacgctggct tatcacaaga aaggccagat
     4501 cgaatacatt ccgttcccgg ataagctgaa aggccgctac caggcgttca ctcaggcaga
     4561 tctgacaaat ctgcgcgcgg cgggttacga caaaccgttc aaaaccgttg ctgaaggtgt
     4621 aacggaatac atggcctggc tgaatcgcga cgcataagag ctctgcatga aaatactggt
     4681 gatcggcccg tcttgggttg gcgacatgat gatgtcgcaa agtctctatc gcacgctcca
     4741 ggcgcgctat ccccaggcga taatcgatgt gatggcaccg gcatggtgcc gtccattatt
     4801 atcgcggatg ccggaagtta acgaagctat ccctatgcct ctcggtcacg gagcgctgga
     4861 aatcggcgaa cgccgcaaac tgggtcatag cctgcgtgaa aagcgctacg accgcgccta
     4921 cgtcttacca aactccttca aatctgcatt agtgcctttc ttcgcgggta ttcctcatcg
     4981 caccggctgg cgcggcgaga tgcgctacgg tttactcaac gatgtacgcg tgctcgataa
     5041 agaagcctgg ccgctaatgg tggaacgcta tgtcgcgctg gcctatgaca aaggcattat
     5101 gcgtaccgca caagatctgc cgcagccatt gttatggccg cagttgcagg tgagcgaagg
     5161 tgaaaaatca tatacctgta atcaattttc gctttcatca gaacgtccga tgattggctt
     5221 ttgcccgggt gcggagtttg gtccggcaaa acgctggcca cactaccact atgcggaact
     5281 ggcaaagcag ctgattgatg aaggttatca ggtggttctg tttggctctg cgaaagatca
     5341 tgaagcgggc aatgagattc ttgccgcttt gaataccgag cagcaggcat ggtgtcggaa
     5401 cctggcgggg gaaacacagc ttgatcaagc ggttatcctg attgcagcct gtaaagccat
     5461 tgtcactaac gattctggcc tgatgcacgt tgcggcggcg ctcaatcgtc cgctggttgc
     5521 cctgtatggt ccaagtagcc cggacttcac accgccgcta tcccataaag cgcgcgtgat
     5581 ccgtctgatt accggctatc acaaagtgcg taaaggtgac gctgcggagg gttatcacca
     5641 gagcttgatc gacattactc cccagcgcgt actggaagaa ctcaacgcgc tattgttaca
     5701 agaggaagcc tgacggatgc gggttttgat cgttaaaaca tcgtcgatgg gcgatgttct
     5761 ccatacgttg cccgcactca ctgatgccca gcaggcaatc ccagggatta agtttgactg
     5821 ggtggtggaa gaaggggtcg cacagattcc ttcctggcac gctgccgttg agcgagttat
     5881 tcctgtggca atacgtcgct ggcgtaaagc ctggttctcg gcccccataa aagctgaacg
     5941 caaagcgttt cgtgaagcgc tacaagcaga gaactatgac gcagttatcg acgctcaggg
     6001 gctggtaaaa agcgcggcac tggtgacgcg tctggcgcat ggcgtaaagc atggattgga
     6061 ctggcaaacc gctcgcgaac ctttagccag cctgttttac aattgtaagc atcatattgc
     6121 aaaacagcag cacgccgtag aacgcacccg cgaactgttt gccaaaagtt tgggctatag
     6181 caaaccgcaa acccagggcg attatgctat cgcacagcat tttctgacga acctgcctac
     6241 agatgctggc gaatatgccg tatttcttca tgcgacgacc cgtgatgata aacactggcc
     6301 ggaagaacac tggcgagaat tgattggttt actggctgat tcaggaatac ggattaaact
     6361 tccgtggggc gcgccgcatg aggaagaacg ggcgaaacga ctggcggaag gatttgctta
     6421 tgttgaagta ttgccgaaga tgagtctgga aggcgttgcc cgcgtgctgg ccggggctaa
     6481 atttgtagtg tcggtggata cggggttaag ccatttaacg gcggcactgg atagacccaa
     6541 tatcacggtt tatggaccaa ccgatccgag attaattggt gggtatggga agaatcaagt
     6601 agagtgtcgt tctacaagta tgtctcttgc agatttgcca gctcaaaccg tttttcaaaa
     6661 tttaaatctt gaaataataa ccaataagtt gacatcggag ataagatgac ctcaacatta
     6721 tttttctctc tcgagaaaaa aaactggata gcgtactgga acagagctct cgtattctta
     6781 ttcattacca cctatttttt gggtgggatt acaaggtata aacatcttat tgttattctt
     6841 atgacaataa cgacaatcgt ctatctctgc aaacggccaa aacactatct ctcactattt
     6901 aaaacatttc tttttggtag tgttgccata ttaactattg ctgcattgct gtcacttctt
     6961 caatcccctg atgcaggtgc tagcatgaag gaaattttca aagctattat tgagaatact
     7021 ttactatgca caatagcaat accggtcata ttgagagacg agaaaagaga agatgtcgaa
     7081 aaaatcgttt tcttctcatt tattagtgcg ttgggcttac gctgtttttc tgaattgatt
     7141 acctattata aggactatca acaagggata atgccattcg cagattatag acaccgtagc
     7201 atttctgact cgatggtctt tttattccct gcattgttaa atctctggct tatcaaatca
     7261 gcaaaatacc gcatttcttt tgtggttcta agcgttattt ttatttttct gatattagga
     7321 actttatcca gaggggcctg gctttccgtg ttagtcattg gattaatatg gattctgatg
     7381 tttaaacaat ggaagttact attagtagga gtaatggttt ccatcattgc attgtcggtt
     7441 attttcacac ataaggagat gaccgcaaag ctaacgtata aacttcaaca aactaatagt
     7501 tcttatcgct atgcaaatgg tactcaaggc agcgcactcg atctaatatt agaaaatcct
     7561 gttattggtt atggttacgg taacgttgca tataaagatg tctataataa acgtgtcatt
     7621 gattatccag aatggacctt tagacaatca atagggccac ataattttgc gctattcatc
     7681 tggtttggca ctggtttatt agggctggta agtcttatga tgctatactg tgcaatattg
     7741 aaagagtgta taaaaaatgg cgtcaagaat aaatatcgct caccatataa tgcatattat
     7801 ataatcttac tatcttttat aggttatttt gttatccgtg gaaacgtaga acaaattgaa
     7861 ccaaatttat taggcgttta cgccggctta ttattagcga tgaaaaacaa gtaaaaaata
     7921 aaaaaggctg cataatgcgg cctttttatt tattcaaacc aattatgaat aacctcttcg
     7981 aatctttgag ttacgccatc ccaactgtag tgatcaaaca caaagtcctg accttgcttt
     8041 gcaactgctg ttaattcagg attcgctaac gtttttaaaa tatcgctgct tatggagtct
     8101 gcagtcatag gttcttttaa atggaatccc gtagtatttt ctttgacaaa ttccgtcatc
     8161 cccccacgcg tactcactaa aacaggctta ccagcaccca tagcttcaat tgccaccatg
     8221 caaaatggtt cctggaactg ggaaggaatg acgactaaat ctgccagtgg gtaataacaa
     8281 tgcatctttt caggcggtat gcttcctaac ataatgcaac gatccttcaa acgttttgca
     8341 atttccctga cattgcgttg atatgcacct ttgtcacttt tactcatttc tgtataatca
     8401 ccaatcacaa ctaattttaa atttttgtga gcagcggcca atttttcgaa tgattgcatc
     8461 aacagcaata tgcctttgtc agggacgata cgcccagcat aaaaaataat tttctcttca
     8521 ggggagatat taagttcgct tctagtgatc ggctgaaaat tagattgata ggtttctaaa
     8581 tcaattccat ttggtacaat ctcaatatct gcatttgcta aataagattg gtaatacttt
     8641 ttcaagtata aactagggac tatcatttta acattttgct caagcaattt tggttcaaat
     8701 gcattatgca tatgtataac cactcttgcc tgaggagctc gcttacgaat ttgagtgtat
     8761 agcttcatgc tattatgaac tacaataaca ctttctttcg ttataggaaa atcatgtgca
     8821 atattcaata ttctctgtga atatggaaga ggatcaagtc ttgtccattt ttgaaataac
     8881 cgcttatata tgcgactgaa tccaacccta tgaatactac aattatcatt aacaaaggtg
     8941 taatttgagt atcctggatt tttgatacaa acaatacgat ttggaaaatt ggtacgctga
     9001 gcaacctgat acatccacgt ttcaacagca gccgctccgc gaggaggaat tgaaaaaata
     9061 ggagtaaccg taaatattat tttatcaacc atctcgtata ttaacctttc ataacattat
     9121 atttaattgc tgtaaaaaca ccatcgagaa attttttctg gtaaagcaag tgtttatatt
     9181 tttctttgtg ctcatgtttt ttaacagctt ttttataagg tatatttctc catggtgata
     9241 tattataaat attacgaaaa taatctgcag aggcgtagtt cgcccaatca tgccatggct
     9301 tagttatacc agtataatgt atgaagacag tgtcatcatt aataaaccgg gtgtaatatt
     9361 cactattttt ttcttcaaat tctgacttta tagtataaat acaattatat tttctcggta
     9421 atattttagc atgatgtaga aacattaagt ttaaaatatc ttgatccggg tactttaatt
     9481 tttttataat tgcctcatct gataatagat caaagaattt ttccgttagt ctttgtttta
     9541 accattcccg taaattaata tacattacac cagagttaaa atagctaccg ttaaaatcct
     9601 cattacacaa acgctcagcg cttttacttt gcatagcatc tacatcaatt accactgccc
     9661 catattcatc tttaaactct aatgctatta actcgttcaa tgaaccctta cagacgatat
     9721 cagcatcaag atataataat gaatccaatc gcgcagaaaa ataatcaaat gaaagcaaac
     9781 gataataaat agatactggc cagatatttg attgaggtag ctccttcaat ggctcagaat
     9841 caatatgata taatttaatt actgtttgat acttcgttgc taattgtgat aataattcaa
     9901 tatactcttt gtctaagtaa tcagcgatta tgtgaaaggt aaatttgttt tgtttattat
     9961 tttcaagaat tgaagcaatt gaaatcgcag ccccaacctg gtagttctca tcaatacccc
    10021 atgagacatt aaacgtttcc cgttcatcaa gtttaacggg gcgcttgtcc agttcaatta
    10081 tatctttaaa ttgagtaaga tgtttaaaat ccaatttaat tacccttcat cataatcatt
    10141 atttgctctt caatcctagt ttgactttta catccctgat tactttttta attttctttt
    10201 tgaatataag atatgtatag ccaaagtctt tcaattcatt ttttatatta tagtgccgtt
    10261 caagatcaat gcgatctttt gcttttaaaa cagcggttgt ttttttacct gaaaggtcaa
    10321 ttagtcttaa acctttttcg gatactataa aatttccttt atgaggatcg ccagacacca
    10381 ttccatgctg atgcaactct tttatcgatt ctgataattg atccttcaga tcttcagaaa
    10441 tttccaaata ctcatttaac ccgacacctt caatatactc aatgagcata atataataat
    10501 gagcgaaatt taatgtttta cgttcagcta gcaaaaaata gtcattaatt gattggatgc
    10561 cttcaccacg tacccgatcg gtttgataaa ttaaattttc ataataatct ttttttatgc
    10621 aagattttaa gaaacgttcg gtcattttat gctttgccgc ataaacttta agtacaagag
    10681 gtccacgagc agtgtcaata agaatgactt tagtatcatc aatagaacga aaaactttta
    10741 atgttttaac cctgcaagtt aaaaagtcat ttagaacttg ctcataaaag ggatcattat
    10801 cttttatgaa aactttcaac ccatttatcg ttttattata tatcataatg aattatttta
    10861 accttaaatc tttagaagca tttttcttta taatacttta aataattaaa aatgcctgcc
    10921 atataatgct tttgtttaaa tttatgtttt gcacaatacc gatactgatt gctatttaca
    10981 ggtttaagta aatcttcttt actccacgga gaagctgctt tggcgatcaa aaaactacgt
    11041 gaaactggat aatcagccca ctcatgccag ggttttgtag gtcccacata gtgtataaac
    11101 acggtgtcat cattgactgg attatcaact ttgtctttta attcatagtt aatactatat
    11161 cgggtattat atttctccga aataaacttc actttaccat tcaataatac attcagtaca
    11221 tcttgatcaa ggtgggtgat tttacttacc caatctgggt cacgcaacat ttcaatagct
    11281 tttgacgaaa tgttatttag attccactca tcaatattta tcagtaaaaa accagcattg
    11341 aaatatccag aagctaactg tggtgtagtt aataccgagg ctcgattttg ccaccattca
    11401 atatctcttt cagctacaac tgcggcaatt tcattagtag aaaattgata atctaagagt
    11461 tctttaatac tacccttgca agcaatatct gcatcaagat atagtatttt ttcatgttta
    11521 tgatagaaat aatctgcaat tataaatcga aaatatgttg cgtaagtcca gtttttcgtg
    11581 cttggtaatg acttcagctt atcacaattg ataacataaa tattaattct taaattatat
    11641 tgttttgcta aatcagaaaa atataatttg tctttatcac tgatataatc tgtgaaaaca
    11701 tgaaattcac aagagatttc tctattgttt aatagaatag atgcgatggc tacaccacat
    11761 ccaaaaagaa aatttctgtc aataccaaat gctatatcaa gagtattttt agatcttata
    11821 ttttgataat gaaaattata ctcaaaaagg atgatgtcac tatcattgag ttgagacata
    11881 tttattctgt atccaaaatt ttggcaaaaa taagttacgg gttacaacga ttttcgaatc
    11941 gttctttccc tgatttttgt ggcttttgct tctgcttgcg acagcagtcc ttgttcctgc
    12001 ttgagaatgt ctttaagcgg ggcggcaaaa tacaccttca taaagcgcca gatatcccgc
    12061 tgagtcaggc caatattcat tgaagaaaaa taaagcccta taagatcttt atcgcgccaa
    12121 cgacgtggaa cgcgcgtgcg aagctgcgcc cggtgtaggt caattaccga aatttttaac
    12181 tcctcttcct taccggaaaa aggcaagtgc agcaggaaat gacagatata acagtcacga
    12241 tggttaatgc ccgcagcatg catatcgcgc accatcgtcg cgacacgctt aataagcata
    12301 cgctttacgc gaacatctgg tgggttggtc gcccagtcag cacagtaatc ttccagactg
    12361 atggttggtg tcagatcttc ggtaataata aacgaggttc tggtcagcgg attcatgcct
    12421 ttttcgccga atgccacccc atacatagta tcaacgccga catcccgcag tcgatgaatc
    12481 gcattccatt cgcggtctgc gcctaatact ggcatccgca atgagagtaa atttttgatt
    12541 atctctttca gggtcgtgcc gcgatgccat ttgagaaaat agctttttcc cgccatttca
    12601 aagcgcagag tacggcgagt ttccagttca cgaaatacct caccctgcaa ggttttaact
    12661 tcctcaaaag gatctttgcc gcgccataac gtggcaaacg gctctttaag ttcaaccatc
    12721 cagaccaccc gttatgatat ccgccgcttt ctctggcaga ctgtataaat cttgtgtatc
    12781 agcataatgt cgcgcatttt ccgcccaagc ctggcgcaat gaagattgcg ttaacgcttt
    12841 gcgtaaaatc tcattcaatg tttcctggcg gaatggctca gcaatagcct cgccgcaatt
    12901 agcgtcgaca atataatgcg catagccaca aacggcagtt gttagtaccg gtaatcctgc
    12961 agtaatcgct tccagcagca caattcccgc cgcttcctgg taggcaggat gcagtaataa
    13021 atccgccgcc gccattaatt ccgagacatc gttgcgcccc gagaagaagt gaacattact
    13081 gcgcacgccg cgtttttctg ccagtgcctc aaattttcgc ggtttatcct gcccaacaac
    13141 atatagcaat gtgttgtggc gcagcgaatc cggtaacgaa gcaagtgctt caatggaacg
    13201 atcgacacct ttacgcgtga agtctgaacc gacctgcaac aataaatatt gttgttcggt
    13261 tattccattc ttcttacgga agatttcacg gctattggct ggctgctggc tatatttacg
    13321 atcaggataa atccccggtg gcagaatatg aaaacgctcc gcttcagtct gataatgttt
    13381 ctggaaatcg gcgatttgct tatctgtcag catcagcagc tgtgtcggct tgctctgttc
    13441 gaaggttgcc cgctcaaagg cggcataatg gcgataacgt gacgtcaggc gatagaaaaa
    13501 gcctttttcc tgcgctactt tctcggcata acaaacatca gcggcataat aaacgtccag
    13561 ccccggcatt ttgttgaatc caacgacttt atcgacggga tgttcgcgta aatgtttttg
    13621 cacccaggca aaatactccg cattgcgccc gtgattggta tgcgatttaa ccggcacttt
    13681 gatcagttca aatacatcag ggcattcgcc ttcccacgac tgggtataaa cccgaacatg
    13741 atgacctcgg gcggcgactg tctgagcaat acgcataaaa tcgcgctgca aaccgccaaa
    13801 gggaaaatat ttatataaac aaaaagcaac gatcataacg acgtacctgt cgtggaggag
    13861 ggcagtaatt tatcgacagc agcaatgaca tctgccgccg gaataaccga aagatacatc
    13921 tcatttcggt cacgctgatc gcgcgttggc atttcccggt aatctcccgc ccagaattga
    13981 atcatgttat ttgaccaggg acgccagaaa atatgatctg ttgcgccaaa cagcgatatc
    14041 agcggcgtat taactgcagc ggcaatatgc gccggtgcgg aatcaacgcc aataaacagc
    14101 tgcgcatgat cgattaacgc accaagttcc gggaaggtca cctttccagc cagcgccgtt
    14161 actggtggcg tctggcatcc ctgcgcaatt tcattgacgc aggccagatc gtctttatct
    14221 gggccggacg tcagaacgac ttcataacca cgagcatgta aggcatcaat cacagcggaa
    14281 aacttggcgt tgtcccagca tttgaagatt tgccgcgccg taggttggat aaccacataa
    14341 ttttgtccaa caccagcgtg atcaagttcg cgacgcatac gtttccagct tgcaggcggg
    14401 taactcatgg ttgtctgctt caccaacgaa tcaagtccca atggtgtcag cacggataag
    14461 ttactttcca ccacatttcc atcctgcaac ggcaccaaat gggtgaaact tttacgccaa
    14521 aaagcagact gccgatgatg ataatcctgg gaaattttca cacgagcatt taataagcga
    14581 accagtatag caaccatcca ttgatcggta agattgacga taaggtcata cttattggca
    14641 cgtaatacct tgatgagatg aaaaaagttg gcaatttttt ctgaggcttt tgctttttta
    14701 ttttttatgc cgtagagcgc gttaatctct ggattttcag acaggatcgg gatggtgtcc
    14761 tgataaagca gcacatcgat ttttgcgtca aggtaatttt ttttcagcga actaatgacg
    14821 ggagtagtta ataacatatc cccatgaaaa cgcatcttaa tgagcaaaat tcttcgaaat
    14881 ggcttatcca caattaactc tttttgttga gattatccga ttaaggtgaa gcagaaaaag
    14941 cacgctaccg ccccaggctc aacagctacc tgaatactga taccgcactg atattgacgc
    15001 gctagtgtat cacttctttc gcggcaatcc gacccgaatg atttttaagc acattgttga
    15061 ttttcaataa agaaatcaat caaataaaat cattagcaac catattcttt cactgttgct
    15121 ctgcgattcc ttcctgcacg tacagcagaa atcaatgaat cgcataaaaa tacaaaaaaa
    15181 gtatagatgt tcagcaagaa ttggcgggta aattgctatt taagtcaaaa acgggaaaag
    15241 taatggtaaa gccacagcta aatacataga atccccagca catccataag tcagctattt
    15301 actatgctcg aattgcttta caccgccctt ctctacctta ttcagccgct gatctggata
    15361 cggctctggg tgcgcggacg taaggctccg gcctatcgaa aacgctgggg tgaacgttac
    15421 ggtttttacc gccatccgct aaaaccaggc ggcattatgc tgcactccgt ctccgtcggt
    15481 gaaactctgg cggcgatccc gttggtgcgc gcgctgcgtc atcgttatcc tgatttaccg
    15541 attaccgtta caaccatgac gccaaccggt tcggagcgcg tacaatcggc tttcgggaag
    15601 gatgttcagc acgtttatct gccgtatgac ctgcccgatg cgctcaaccg tttcctgaat
    15661 aaagtcgacc ccaaactggt gttgattatg gaaaccgaac tatggcctaa cctgattgcg
    15721 gcgctacata aacgtaaaat tccgctggtg atcgctaacg cgcgactctc tgcccgctcg
    15781 gccgcaggtt atgccaaact gggtaaattc gtccgccgct tgctgcgtcg tattacgctg
    15841 attgctgcgc aaaatgaaga agatggtgca cgttttgtgg cgctgggcgc aaaaaataac
    15901 caggtaaccg ttaccggtag cctgaaattc gatatttctg taactccgca gttggctgct
    15961 aaagccgtga cgctacgccg ccaatgggca ccacaccgcc cggtatggat tgccaccagc
    16021 actcacgaag gtgaagagag cgtggtcatt gccgcacatc aggcattgtt acagcaattc
    16081 ccgaatttat tgctcatcct ggtaccccgt catccagaac gcttcccgga tgcgattaac
    16141 cttgtccgcc aggctggact aagctatatc acacgctctt caggggaagt cccctccacc
    16201 agcacgcagg ttgtggttgg cgatacgatg ggcgagttga tgttactata cggcattgcc
    16261 gatctcgcct ttgttggcgg ttcactggtt gaacgtggtg ggcataatcc gctggaagct
    16321 gccgcacacg ctattccggt attgatgggg ccgcatactt ttaactttaa agacatttgc
    16381 gcgcggctgg agcaggcaag cgggcttatt accgttaccg atgccactac gcttgcaaaa
    16441 gaggtttcct ctttactcac cgacgccgtt taccgtagtt tctatggccg tcatgccgtt
    16501 gaagtactgt atcaaaacca gggcgcgcta cagcgtctgc ttcaactgct ggaaccttac
    16561 ctgccaccga aaacgcattg aggttgttat gcaaaaacgg gcgatttatc cgggtacttt
    16621 cgatcccatt accaatggtc atatcgatat cgtgacgcgc gccacgcaga tgttcgatca
    16681 cgttattctg gcgattgccg ccagccccag taaaaaaccg atgtttaccc tggaagagcg
    16741 tgtggcactg gcacagcagg caaccgcgca tctggggaac gtggaagtgg tcgggtttag
    16801 tgatttgatg gcgaacttcg cccgtaatca acacgctacg gtgctgattc gtggcctgcg
    16861 tgcggtggcg gattttgaat atgaaatgca gctggcgcat atgaatcgcc acttaatgcc
    16921 ggaactggaa agtgtgtttc tgatgccgtc gaaagagtgg tcgtttatct cttcatcgtt
    16981 ggtgaaagag gtggcgcgcc atcagggcga tgtcacccat ttcctgccgg agaatgtcca
    17041 tcaggcgctg atggcaaaat tagcgtagcg tttatgccgg atggtatgcc atccggctcg
    17101 catgaattac ttctggcact gccgacaata aaacgttgcc cgctgcgcat gtttagtcgc
    17161 cacaatcggc gtaccgcaca cccgacacgg ctcacctttt cgcccgtaaa cctgcaattc
    17221 ctgcgcgaaa tagcccggtt taccatcact ttgcagaaaa tctttcagcg ttgtaccacc
    17281 ctgctcaatc gaacgcagca acaccgcttt aatcacccga gctaacaatt cacactctgc
    17341 cagcaacagt gatgacgcca gccgatccgg atggatcccc gccgcaaaca gtgattcgct
    17401 ggcatagata ttccctaccc ctaccaccag cttgttatcc atcagccacg gtttaatcgc
    17461 cgtttttttc ttcgcgcact tctgatgcag atactcacca ttgaaatcgt cgctaagcgg
    17521 ctccggtcca agatgggcca gcacattatg cccttccagc tctttggtcc acagccaggc
    17581 accaaagcgg cgcggatcgg tgtagcgcag cactttaccg ttgctcatca ccaaatccac
    17641 atggtcatgc ttttcagggg gaagttcttc tggaaggatg cgcaggctgc cagacatccc
    17701 caaatgaatg ataatccagc cctcaggcag ctccagcagc agatatttag cccgccgctg
    17761 cacgctaagc actggttgat cgcttaatcg gtagatctct tctgaaaccg gccagcgcaa
    17821 gcgtccgttg cgcaccaccg catgaagaat ggttgcacca acgagatgcg gttctatgcc
    17881 gcgacggctg gtttcaactt cgggtaattc aggcatagca tctccaggaa tgaacagatg
    17941 caatcaatat gggggcaagc agataacaaa aaaccccgcc ggagcgaggt ttttcgttac
    18001 atcaaagcga gaattatttg attttcgctt ctttgtagat cacgtgctga cgaacaactg
    18061 gatcgaattt tttcagttcc agtttttccg gcttagtacg tttgttcttc gtagtggtat
    18121 agaagtgacc agtaccagca gaagaaacca gcttgatttt ctcacgaata cctttagcca
    18181 tgatttattt cctctaagta cttagtactt ttcgccacgg gcacgcagtt cagccagaac
    18241 tgtatcgatg ccttttttat cgattacacg cataccttta gcagatacgc gcagggtgac
    18301 aaaacgcttc tcgctctcaa cccagaaacg gtgagagtgc aggttcggca ggaaacggcg
    18361 tttagtcgcg ttcagtgcgt gggaacggtt gttaccggtc accggacgct tgccagtaac
    18421 ttggcagact cgggacatgt ctattctcca aaaatcaaat tagctcgagc ttcgtatggg
    18481 gtattggcgc ctcgtcaggc tttacagccc ggtcatcgca gttctatgtg aactctcgat
    18541 tgccaggccc aaatgccaaa cccgagattc tcaaaggtgg cgtagtatac gctgactcag
    18601 cgatgtgctc aagtcccgaa cagacaaaga tcccgaagga tcgcgcatag cgggttaaat
    18661 ccagccgcgt tcggcaaaag aaacatactc tccacgccca atcacgatat ggtcgagcac
    18721 gcgtaaatcc atgaactgac aactctttat tatccgttca gtaatgagtt tatccgcttt
    18781 actgggttca gcacaacccg aagggtgatt atgtgcaagg atcagcgccg aggcgtttat
    18841 ttttatcgct tcgcggataa tttcccgagg atggacttca acatggttta gcgtgccgga
    18901 aaaaagacgg ctatgcgtta taacccggtg ttgtgagtcg agaaagatca ccataaagat
    18961 ctcccgctcc tcacccgtga gctggctttg taaaaattcc cgcgtcatct ccgggctgag
    19021 taaagggctt tcttcacgca tccgcacgtt gtagtaacgt cgcgccagtt cagcaatccc
    19081 ctttaactgg gcaaatttcg ctacgccaat tccatgaaca ccactaaatt gttcatattc
    19141 agaggttaac aagccataaa gagagccgaa attctccagc atctcttttg ccagggttaa
    19201 tacatcttta ccgcgcgttc cggtacgcag aaatagcgcc agcagctcga catccgttaa
    19261 ggcgctaata ccaaacttca gcattttttc gcgcggcatc aacagctgtg cattgttttt
    19321 caccttcacc tcctttgtgg tgctcgcatc ctgtcatagc cgttccgtta aatcgacggc
    19381 cagttttcaa tcctggaaag agcctcgcaa agtgaatctc aggtgaaggc acgacaacaa
    19441 aagtattgtg ataaaatcgc caacttctgg tgtcacccaa caggaaaaat catcatgagc
    19501 ctggccggta aaaaaatcgt tctcggcgtt agcggcggta ttgctgccta taaaacccct
    19561 gaactggtgc gtcgtttgcg cgatcgcggg gccgacgtcc gcgtagccat gaccgaagcg
    19621 gcaaaagcct ttatcacccc acttagcttg caggcggttt ctggttatcc cgtttccgac
    19681 agtctgcttg acccggcagc cgaagccgct atgggccata ttgagctggg taaatgggct
    19741 gatttagtga ttctcgcccc tgccacggca gatttgattg cccgtgttgc tgccggaatg
    19801 gcgaatgacc tggtatcgac gatttgtctg gctacacctg cgcctgtagc cgtgctcccc
    19861 gccatgaacc agcagatgta ccgtgccgct gccacgcagc ataatttaga ggtgcttgct
    19921 tcccgtggtt tgctcatctg ggggccagac agtggcagtc aggcttgtgg tgatatcggt
    19981 cctgggcgaa tgctcgatcc gttaaccatt gtggatatgg cggtggcgca tttttcgccc
    20041 gtcaacgacc tgaaacatct gaacattatg attaccgccg gtccgacgcg tgaaccgctc
    20101 gatccggtgc gttatatctc taatcacagc tccggcaaga tgggttttgc tatcgccgcc
    20161 gctgctgccc gtcgtggcgc gaacgtcacg ctggtatcag gtccagtttc actaccgacg
    20221 ccaccgtttg ttaaacgtgt tgatgtgatg accgcgctgg aaatggaagc cgccgtgaat
    20281 gcttctgtac agcagcaaaa tatttttatc ggttgcgccg ccgtggcgga ttatcgcgca
    20341 gctaccgtgg ccccagaaaa aatcaaaaag caggccacgc agggtgatga attaacaata
    20401 aaaatggtta aaaaccccga tatcgtcgca ggcgttgccg cactaaaaga ccatcgaccc
    20461 tacgtcgttg gatttgccgc cgaaacaaat aatgtggaag aatacgcccg gcaaaaacgt
    20521 atccgtaaaa accttgatct gatctgcgcg aacgatgttt cccagccaac tcaaggattt
    20581 aacagcgaca acaacgcatt acaccttttc tggcaggacg gagataaagt cttaccgctt
    20641 gagcgcaaag agctccttgg ccaattatta ctcgacgaga tcgtgacccg ttatgatgaa
    20701 aaaaatcgac gttaagattc tggacccgcg cgttgggaag gaatttccgc tcccgactta
    20761 tgccacctct ggctctgccg gacttgacct gcgtgcctgt ctcgacgacg ccgtagaact
    20821 ggctcagggt gacactacgc tggttccgac cgggctggcg attcatattg ccgatccttc
    20881 actggcggca atgatgctgc cgcgctccgg attgggacat aagcacggta tcgtgcttgg
    20941 taacctggta ggattgatcg attccgacta tcagggccag ttgatgattt ccgtgtggaa
    21001 ccgtggtcag gacagcttca ccattcaacc tggcgaacgc atcgcccaga tgatttttgt
    21061 tccggtagta caggctgaat ttaatctggt ggaagatttc gacgccaccg accgcggtga
    21121 aggcggcttt ggtcactctg gtcgtcagta acacatacgc atccgaataa cgtcataaca
    21181 tagccgcaaa catttcgttt gcggtcatag cgtgggtgcc gcctggcaag tgcttatttt
    21241 caggggtatt ttgtaacatg gcagaaaaac aaactgcgaa aaggaaccgt cgcgaggaaa
    21301 tacttcagtc tctggcgctg atgctggaat ccagcgatgg aagccaacgt atcacgacgg
    21361 caaaactggc cgcctctgtc ggcgtttccg aagcggcact gtatcgccac ttccccagta
    21421 agacccgcat gttcgatagc ctgattgagt ttatcgaaga tagcctgatt actcgcatca
    21481 acctgattct gaaagatgag aaagacacca cagcgcgcct gcgtctgatt gtgttgctgc
    21541 ttctcggttt tggtgagcgt aatcctggcc tgacccgcat cctcactggt catgcgctaa
    21601 tgtttgaaca ggatcgcctg caagggcgca tcaaccagct gttcgagcgt attgaagcgc
    21661 agctgcgcca ggtattgcgt gaaaagagaa tgcgtgaggg tgaaggttac accaccgatg
    21721 aaaccctgct ggcaagccag atcctggcct tctgtgaagg tatgctgtca cgttttgtcc
    21781 gcagcgaatt taaataccgc ccgacggatg attttgacgc ccgctggccg ctaattgccg
    21841 cgcagttgca gtaatatgac gccggatgac ttttcatccg gcgagtttct ttaaacgcca
    21901 aactcttcgc gataggcctt aaccgccgcc agatgttccg ccatttccgg cttctcttcc
    21961 agataggcaa tcagatcttt cagggtgatg atagagatca ctttgcagtt gtaatcacgc
    22021 tcaacttcct gaatcgccga aatctcgccg cgcccgcgtt cctgacgatc gagcgaaatc
    22081 aacacgccag caagcgtcgc gccattggcc tgaataatct ccatcgactc gcgaatcgcc
    22141 gttccggcgg tgatcacatc atctaccagc attacgcgtc cttgtaacgc gctaccaacc
    22201 agattgccgc cttcaccgtg gtcttttgct tctttgcggt taaagcagta cggcaggtcc
    22261 aagtcgtgat gctccgccag tgccacagcg gttgtggtgg caatcgggat ccctttgtaa
    22321 gcagggccaa acagcagatc gaactcaatg ccggaatcca ccaacgcttc agcgtaaaaa
    22381 cggcctaaca gtgccagatc gcgcccggta ttaaacagcc cggcgttgaa gaaatagggg
    22441 cttttgcgcc cggatttcag cgtaaactcg ccaaacttta acacctgctt gctaagcgca
    22501 aattcaataa actggcgctg atatggtttc atgccttcgc tcctcatctt acttttctac
    22561 agacaaaaaa aaggcgactc atcagtcgcc ttaaaaatca gtttgccagc gccgccttct
    22621 gcgtcgctac aatggattcg attccccctc gggccagcgc caacaaggtg agtagctctt
    22681 catgggtgaa cggctcgcct tctgccgtcc cctgcacttc aatgatgcgc ccgtcttcgg
    22741 tcatcactac gttcatgtcg gtctctgcgg ctgagtcttc aacgtattcc agatcgcaaa
    22801 ttgcttcacc gtttacaata ccgacagaaa ctgcggctac catccctttc atcgggttgg
    22861 ttttcagctt gccgttttcc accagcttct gtagcgcatc tgccagcgcc acgcaggcac
    22921 ccgtaatcga cgcggtacga gtgccaccat cagcctgaag cacgtcgcag tccagcgtaa
    22981 tggtgaactc acccagcgct ttcaaatcta ctgccgcgcg aagagcacgg gcgatcagac
    23041 gctggatttc cattgtgcgt ccaccctgct tacctttcgc cgcttcacga gcgttacggg
    23101 tgtgggtaga acgtggcagc atgccgtact ctgcggtgat ccagccctgg ccctgacctt
    23161 tcaggaagcg cggcacgcct tcttcaatag aggcggtaca caatactttg gtatcgccaa
    23221 attcgaccag caccgagcct tctgcatgtt ttgtatagtt acgagtcagg gtaacgggac
    23281 gcacctgatt attgctacgg cctgctggac gcatattgaa atctccggct tgaaacaaat
    23341 gtggctgcgc attatacgga cttccggcgg ttattcctat cctgacaagg catcgatggc
    23401 tataatcctt ccacctctcc ttttataaac aggaacgtct atgatccgca gtatgaccgc
    23461 ctacgcccgg cgtgaaatca agggtgaatg ggggagcgca acctgggaaa tgcgctcggt
    23521 aaaccagcgt tatctggaaa cttactttcg tctgccggag cagttccgta gccttgaacc
    23581 tgtcgttcgc gagcgtattc gttctcgcct gacgcgcggt aaagtggaat gtaccctgcg
    23641 ctatgagcca gatgttagcg cacaaggtga gctgatcctc aatgaaaaac tggctaaaca
    23701 gctggtaact gccgcgaact gggtaaaaat gcagagtgac gaaggggaaa tcaacccggt
    23761 tgatattcta cgctggccgg gcgtgatggc agcccaggag caggatctcg acgcgattgc
    23821 cgctgaaatt ctcgcggcgt tggatggtac gctggacgac tttatcgtcg cgcgcgaaac
    23881 cgaaggtcag gcactgaaag cattgatcga gcagcgtctg gaaggcgtca ccgccgaagt
    23941 ggtcaaagtc cgcgcccata tgccggaaat tatgcaatgg cagcgtgagc gtctggtcgc
    24001 gaagctggaa gatgctcagg ttcagttgga aaacaaccgt ctggagcagg agttggttct
    24061 gctggcacaa cgaattgacg ttgccgaaga gctggaccgc ctcgaagcgc atgttaaaga
    24121 gacgtacaac attctgaaga aaaaagaagc ggttggtcgt cgtctggact ttatgatgca
    24181 ggagttcaac cgtgagtcga acactcttgc gtcgaaatct atcaatgccg aagtgacaaa
    24241 ctccgccatc gagctgaaag tgttgattga gcagatgcgc gagcagattc agaatattga
    24301 gtaactcgta acaccaattc tgacttttca gccttcacaa atgccgccca ctcaaacaga
    24361 gcggcatttt tcttccccgc aacttcaatt ctgttttgcg tgcctgctcc agattttgcg
    24421 atgttttttt gcccagcaca ctgagaacgt gagatactca caactgtata taaatacagt
    24481 tacagattta ctttctttgc aatcgatatc acatggagtg ggcaatgaac gaacatcatc
    24541 aaccttttga agagataaaa ctgattaatg caaactgaac cgccccggg
//
LOCUS       DADLSA010000049        23859 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.049, whole genome shotgun sequence.
ACCESSION   DADLSA010000049 DADLSA010000000
VERSION     DADLSA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.049"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..1095
                     /locus_tag="JN691_001981"
     CDS             <1..1095
                     /locus_tag="JN691_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY7243436.1"
                     /translation="VASPAQASVSDLTGDQPAAQPVPVSAPATSTAAVSAPANPSAEL
                     KIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNVEELLKSNTPLNVLALNQPENIENR
                     VNICYFALSPEDEARDAARHIRDQGKQAPLVLIPRSALGDRVANAFAQEWQKLGGGTV
                     LQQKFGSTSELRAGVNGGSGIALTGSPITPRATTDSGMTTNNPTLQTTPTDDQFTNNG
                     GRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATLYASSRSAQGTAGPDFRLEMEGLQY
                     SEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDAWSLANHFSQMRQVQGFEINGN
                     TGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            1053..1448
                     /locus_tag="JN691_001982"
     CDS             1053..1448
                     /locus_tag="JN691_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY7243437.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            1468..2058
                     /gene="diaA"
                     /locus_tag="JN691_001983"
     CDS             1468..2058
                     /gene="diaA"
                     /locus_tag="JN691_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAY7243438.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            2068..2643
                     /gene="yraP"
                     /locus_tag="JN691_001984"
     CDS             2068..2643
                     /gene="yraP"
                     /locus_tag="JN691_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAY7243439.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(2757..3797)
                     /locus_tag="JN691_001985"
     CDS             complement(2757..3797)
                     /locus_tag="JN691_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7243440.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVTMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(3870..4505)
                     /locus_tag="JN691_001986"
     CDS             complement(3870..4505)
                     /locus_tag="JN691_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAY7243441.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            4633..5151
                     /gene="yhbO"
                     /locus_tag="JN691_001987"
     CDS             4633..5151
                     /gene="yhbO"
                     /locus_tag="JN691_001987"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAY7243442.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLATSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(5131..5563)
                     /locus_tag="JN691_001988"
                     /pseudo
     CDS             complement(5131..5563)
                     /locus_tag="JN691_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
     gene            5614..5916
                     /gene="yhbQ"
                     /locus_tag="JN691_001989"
     CDS             5614..5916
                     /gene="yhbQ"
                     /locus_tag="JN691_001989"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAY7243443.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(5903..6406)
                     /locus_tag="JN691_001990"
     CDS             complement(5903..6406)
                     /locus_tag="JN691_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7243444.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(6400..6924)
                     /locus_tag="JN691_001991"
     CDS             complement(6400..6924)
                     /locus_tag="JN691_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAY7243445.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            7133..8128
                     /locus_tag="JN691_001992"
     CDS             7133..8128
                     /locus_tag="JN691_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY7243446.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDDYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            8137..9015
                     /locus_tag="JN691_001993"
     CDS             8137..9015
                     /locus_tag="JN691_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY7243447.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            9096..10103
                     /locus_tag="JN691_001994"
     CDS             9096..10103
                     /locus_tag="JN691_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HAY7243448.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(10221..11465)
                     /gene="mtr"
                     /locus_tag="JN691_001995"
     CDS             complement(10221..11465)
                     /gene="mtr"
                     /locus_tag="JN691_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY7243449.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(11619..13508)
                     /locus_tag="JN691_001996"
     CDS             complement(11619..13508)
                     /locus_tag="JN691_001996"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAY7243450.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGECREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(13501..13581)
                     /gene="yrbN"
                     /locus_tag="JN691_001997"
     CDS             complement(13501..13581)
                     /gene="yrbN"
                     /locus_tag="JN691_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAY7243451.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(13688..14572)
                     /gene="nlpI"
                     /locus_tag="JN691_001998"
     CDS             complement(13688..14572)
                     /gene="nlpI"
                     /locus_tag="JN691_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAY7243452.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(14681..16816)
                     /gene="pnp"
                     /locus_tag="JN691_001999"
     CDS             complement(14681..16816)
                     /gene="pnp"
                     /locus_tag="JN691_001999"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAY7243453.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGCV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(17063..17332)
                     /gene="rpsO"
                     /locus_tag="JN691_002000"
     CDS             complement(17063..17332)
                     /gene="rpsO"
                     /locus_tag="JN691_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAY7243454.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(17481..18425)
                     /gene="truB"
                     /locus_tag="JN691_002001"
     CDS             complement(17481..18425)
                     /gene="truB"
                     /locus_tag="JN691_002001"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAY7243455.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDSLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LCELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(18425..18826)
                     /gene="rbfA"
                     /locus_tag="JN691_002002"
     CDS             complement(18425..18826)
                     /gene="rbfA"
                     /locus_tag="JN691_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAY7243456.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(18990..21662)
                     /gene="infB"
                     /locus_tag="JN691_002003"
     CDS             complement(18990..21662)
                     /gene="infB"
                     /locus_tag="JN691_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY7243457.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNILGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(21687..23174)
                     /gene="nusA"
                     /locus_tag="JN691_002004"
     CDS             complement(21687..23174)
                     /gene="nusA"
                     /locus_tag="JN691_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAY7243458.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(23202..23654)
                     /gene="rimP"
                     /locus_tag="JN691_002005"
     CDS             complement(23202..23654)
                     /gene="rimP"
                     /locus_tag="JN691_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAY7243459.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
BASE COUNT         5628 a         6661 c         6001 g         5569 t
ORIGIN      
        1 gttgccagcc cggcacaagc ctcggttagc gatctgaccg gtgatcagcc tgcggcccag
       61 ccggtgcctg taagcgcccc ggcgacaagc accgcagcgg taagcgcacc cgcaaatcca
      121 tccgcagagc tgaaaatcta cgatacctca tcacaaccac ttagccagat cttaagccag
      181 gttcagcagg atggcgcgag tattgtggtt ggtccgttgc tgaaaaataa cgttgaagag
      241 ttgctgaaga gcaacacccc gctgaacgta ctggcactga accagccgga gaatatcgaa
      301 aaccgcgtca atatttgtta cttcgcgctt tcaccggaag acgaagcgcg cgatgcagcg
      361 cgtcatattc gtgaccaggg taaacaagcg ccgctggtgc tgatcccacg cagtgcattg
      421 ggcgatcgcg tagccaatgc gtttgcgcaa gagtggcaga aactgggcgg cggcaccgtt
      481 ctgcaacaaa aatttggttc caccagcgaa ttacgcgcgg gtgttaacgg cggttctggt
      541 attgctttaa cgggtagccc gattactccc agagcgacaa ccgactccgg catgacgacc
      601 aacaatccaa cgctgcaaac cacgccaacc gatgaccagt tcaccaataa tggcggtcgt
      661 gtcgatgcgg tctacattgt ggcaacgccg ggtgaaatcg cttttatcaa accgatgatc
      721 gccatgcgta acggtagcca gagcggtgca acgctgtacg ccagttcccg cagcgcacaa
      781 ggcaccgcag gcccggattt ccgactggag atggaaggct tgcagtacag cgaaatcccg
      841 atgctggcag gcggtaatct gccgttaatg cagcaggcgc tcagcgcggt gaacaacgat
      901 tattcgctgg ctcgcatgta tgcgatgggc gttgatgcct ggtcgttggc aaatcatttc
      961 tcacaaatgc gccaggttca gggttttgaa atcaacggta ataccggaag tctgacggct
     1021 aacccggatt gcgtgattaa caggaagtta tcatggctac agtaccaaca aggtcaggta
     1081 gtccccgcca gttaaccacc aaacagaccg gcgatgcgtg ggaagcacaa gcgcgtcgct
     1141 ggctggaagg caaaggactg cggtttatcg ccgctaacgt gaacgagcgt ggcggcgaga
     1201 tcgatctgat aatgcgtgaa ggcctgacca ccgtttttgt cgaggtacgc tatcgccgct
     1261 ctgcgcttta tggcggcgcg gcagccagtg tgacccgcag caaacaacac aaattattac
     1321 agactgcccg cttgtggctc gcgcgtcata atgggagttt tgatactgtg gattgccggt
     1381 tcgatgtggt agccttcacc gggaatgagg ttgagtggat taaggatgcc tttaatgacc
     1441 actcataatt aaggtttaag gattagcgtg caagaaagaa ttaaagcttg cttcactgaa
     1501 agcattcaaa ctcaaattgc ggcggcagag gcgcttccgg atgccatctc ccgtgcagcc
     1561 atgacgctgg ttcagtctct gctcaatggc aacaaaatcc tctgttgtgg taatggaact
     1621 tccgctgcca atgcacagca ttttgctgcc agcatgatca accgtttcga aacggagcgg
     1681 cccagcttac ctgccattgc actaaatact gataatgttg tcttaacggc gattgccaac
     1741 gatcgcttac atgatgaagt gtatgcaaaa caggtgcggg cgctgggtca tgcgggagat
     1801 gtattgttag ccatttccac ccgtggcaac agccgcgata ttgttaaagc agttgaagcc
     1861 gccgttacgc gtgatatgac cattgtggca ttgaccggct atgacggcgg cgaacttgca
     1921 ggtttgttag ggccacagga tgtggagatc cgcattcctt cgcatcgtag tgctcgcatt
     1981 caggaaatgc atatgctgac ggtaaattgc ctgtgcgatc tgatcgataa cacgcttttc
     2041 cctcaccagg atgattaagg agaatacatg aaggcattat cgccaatcgc agtccttatt
     2101 tccgcgctgc tgttgcaagg ttgtgttgcc gctgccgtag tgggtaccgc tgctgtgggt
     2161 accaaagccg caactgaccc acgcagtgtc ggcacccagg tggacgatgg taccctggaa
     2221 gtgcgcgtga acagcgcatt gtcgaaagac gaacagatta agaaagaaac ccgtattaat
     2281 gtcacggcct atcagggcaa agtgctgctg gttgggcagt caccaaatgc tgaactttcg
     2341 gctcgcgcca aacagattgc tatgggcgta gacggtgcca acgaagtgta taacgagatt
     2401 cgtcagggcc agccgattgg tctgggcgaa gcatctaacg atacgtggat caccaccaaa
     2461 gtgcgttcgc agctcttaac cagcgacctg gtgaaatcgt ccaacgtgaa agtgaccacc
     2521 gaaaacggtg aagtgttcct gatggggctg gtgactgaac gtgaagcgaa agcggcggca
     2581 gatattgcca gccgggtgag cggcgtaaag cgggtaacca cagcgtttac gtttattaaa
     2641 tagcagcccc ttgtaatgcc tgatgcgacg cttgccgcgt cttatcatgc ctacaacgat
     2701 gcaaaccgta ggtcggataa ggcgtttacg ccgcatccga catgttttcc ctcaaatcag
     2761 aacaacagcg ccagcccgcc gacaatcaca ccagacactg ccaccatcgc ccctgtcagc
     2821 cataaggctt tcgccgggaa cgctttgcgc agcataatca gtgacggcaa actcactgcc
     2881 gggagcgtca tcaacaatgc cagcgccgga gcggttccca tacctgccag catcatcgtt
     2941 tgtacaatcg gaatttctgc tgccgtggga atgacaaaca agcatcctgc taccgccatc
     3001 gtcaccaccc acatcaggct gttatcgaca gcaccatcgg catgggggaa taaccagacg
     3061 cgtgcggcac ccaacaccag tactgcaagg atgtaaaccg ggatcgtact ccagaaaagt
     3121 gtccatagcg ccctgcccca gcggctaaaa aaaccgctct gtgcttccgg tatgtcaatt
     3181 tcgaccggtg cctgcgtttg cggtgtttca cgcacccatt tttgcaccag cgtcgcaatc
     3241 agcaacacca tcaccagccc ggccaccaga cgaatcgccg caaaacccca gccgaggaca
     3301 aaccccataa acaccagcgt cgccgggttt aacaccggat tgcccatcca gaatgccagc
     3361 gcaccgccca tcgatacctg ttggcgacgc attcccgccg cgaccggagc cgcacagcag
     3421 gtacacatca tgcccggcag cgaaaacagc gttcccagca gcgtgccgcg aaagcgcgat
     3481 tgcccaagcg tacgcaacaa ccagtcacgc gggatcagca cctgaatcaa cgaaccgagg
     3541 atcaccccca gcaccgccgc tttccatact gcgaggaaat agatcatcgc gtaatccaac
     3601 gccgcttgcc acggattagc atccgcctgc gcaaggatag atttaccgat actgtgggtt
     3661 tcggctgcag taaacgcttt gccgtagtaa ggttgccatt tcacatacca gagaccggca
     3721 atgacaacga gaaagaaaag cgcgggtttc caccactgaa tgggcgttgc cgcctgagat
     3781 gaagactgac cagtcatagc attccccagg aataagttat gataattgag cgcgtgaata
     3841 ttacgctcac tatcaattct tgggaataat tattcagctc ttttgcgtaa ttctgaagag
     3901 cttaaaatcg tcacgccttc atgctcaggc cgcatcgcct ctgccagcat gacccgcgcc
     3961 acgtctctcg catcaatgga tttccagtta cctggtaaca aacggaacag cggcgcaaaa
     4021 agcgtttcgt tcatccgctg tttgctacga tcgcccagta acatcgacgg gcgagcaatg
     4081 gtcaatttcg gccagttctg ggcaattaat gcttcttcca tctccccttt gacgcggtta
     4141 tagaaaaacg gcgagtgggc attggcaccc atagcactga ccaccagcat atgctgcgcg
     4201 cccagtcgcc gcccggttaa tgcggtatcc actaccagcg tgtaatcggc atgaataaac
     4261 gcctctttgc tccccgcttc tcgccgcgtg gtgccgagac aacaaaacac aatgtcgatg
     4321 ggttcggtga cctgcgccaa cgcgtcactc agttgcggat catggggatt aaacaccccg
     4381 ggcatatcgc ccaacggacg tcgcgtcggc gcagcaatgg cgttaacttt cggttcatta
     4441 atcaacatcc gcagcaggtg accgcccacc agccccgttg cgcctgtaat cagtacctga
     4501 ctcatcttcg ctcctttaca gaattgtctg ccttgcgctc cccggctcat gcaccagtct
     4561 taatagacca tgaggtaatt atccccgatt gtggaaaatt cgcctcatcc aatgcaacaa
     4621 cgcggaggaa gcatgagtaa gaaaattgcc gttttaatca ctgatgaatt tgaagattca
     4681 gaatttactt cacccgcaga cgagttccgt aaagccggac acgaagtgat tactattgaa
     4741 aaacaagcgg gtaaaacggt gaaaggcaaa aaaggagaag ccagcgtgac catcgataaa
     4801 tccatcgatg aagtgacgcc agcggagttt gatgccctgt tgctaccggg tggtcattca
     4861 ccggattatc tgcgtggcga caaccgtttt gtcaccttta cccgtgattt tgtgaatagc
     4921 ggcaaacctg ttttcgccat ctgtcacggt ccgcagctgc tgatcagcgc cgatgtgatt
     4981 cgggggcgca aactgaccgc agttaaaccg atcattattg atgttaaaaa tgcgggcgtg
     5041 gaattttacg atcaggaagt cgtggttgat aaagatcagc tggctaccag ccggacaccg
     5101 gacgatctgc cagcgtttaa ccgcgaggcg ttacgcctgc tcggtgcctg agtcgcgtag
     5161 ccaaatcatt tttttaccaa agcccagcgt gttgtcggtg aatttgattt catcgagccg
     5221 gatttcccac accggggccg acagcattct ggcaaccgga aagcgacgat tgtacgcctt
     5281 gcgcgcgagg tcgctttcct caccttccag cctgcggatc tcacctttaa actgcacacc
     5341 gcgaattaac gctaccgttt tcggctgacc gtttaccgtt agcctgcggc ccactcatct
     5401 gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc gactttctgc gcatcaaaaa
     5461 gataaaaggc attagcgcac cagagttccc cttcctgctg cacacaccag gtgacaacat
     5521 gttgttttgc cagccaacgg ctgatggcga tgagtgtttc cattgctgtt ctcttttata
     5581 ctgtgggcct gaccttaaca tgcgatgcag acgatgacac cctggtttct ttacttgatc
     5641 cgtaccgccg acaataagct ttataccggg atcaccacgg atgtcgaacg ccgctatcag
     5701 cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga aaggagaact gacgctggcg
     5761 ttttctgcgc cagtgggcga ccgttcgctg gcactacggg cggaatatcg cgttaagcaa
     5821 ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg cagggtttgc agaactgttg
     5881 agcagcctgc aaaccccgga gattaaaagc gattgaaatg ctcgtgatac tcaaccagac
     5941 cggttacgcc attcagcgcg tcatccgcta aacgatgtac ctggaaggcg ctttcagtgc
     6001 ccggccagcg gcaacgcaga tcgtgatgcg ccgccagttc aaagccgaaa cgactgtaca
     6061 gcgccggatc gcccagcgtc accactgcgg catagccgaa ctcattaagc gaatcgagtc
     6121 cttcatagac cagttggcgt gccagcccct gtccacggta tttttcatca accgccaacg
     6181 gtgccatgcc gacccattgc aggtcttcgc cctgcacatc aaccggacta aatgccacat
     6241 agccaatgac ctgaccttcg tcatctgtcg ccaccagccc cagcgtcaga aagccatctt
     6301 cacgcagatc gtgaaccagc ttcgcttccg catcactttc gaatgagcga cgcagcaggg
     6361 catcaatacc cggcgcatca atgggaattt ctactcgaat tagcatggtt cacctaccga
     6421 tgtctgtttg gtttcaggcg cggttttcat tcccgcctca acaaaatccg ccagttgcag
     6481 cagcatcatg cgtaaggctt tcggcatttg ctccagttca atggcgtcca tcaggttttt
     6541 cacatacacc cccagctccg tatcgccttc aatcaccagc cgacgctgga agaagagcgt
     6601 atccggatct tgtttacgcg ccgcaatcat cagcagatcg ctggcgtcgg cactaaaact
     6661 cacatcagct tgcgcgttct ggctgaccac cagtttgcca ttcaccaccg aggtaaacca
     6721 ttgcaggtca atatcacgca catgaatact taaccagcgg ccttcaagaa actccagctc
     6781 gccatcatcc agcgcctgac ggaattgcca gcttaagacc tgctcaagaa cctggcgttt
     6841 tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga gatggcccca aatgcacaat
     6901 acgggaacgc agtttatcca acacgagttt tactccctgt ttcaacaatc atcctatttt
     6961 gccatatcag aaaaataaca tagcggtata aatcaacaat tccatatgaa attgctgcta
     7021 ccaccaatac aactttaact gccttaaatc aaaaattgtc gcagcaaggt taactaaaat
     7081 cccagttcgt taacattttt acgttttgat agcgcaacct tcaggaaaaa ttatggagct
     7141 gctctgccct gccggaaatc tcccggcgct taaggcggcc atcgaaaacg gcgcagatgc
     7201 tgtttatatc gggctaaaag atgataccaa tgcccgtcac ttcgccggcc ttaactttac
     7261 cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa catcgccgca aacttcacat
     7321 cgcgattaac acttttgcgc atccggacga ttacgcccgt tggcagcgtg ccgtggatat
     7381 ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac ctcgccatgc tggagtacgc
     7441 cgccgagcgt tacccgcata ttgaacgtca tgtgtcagtg caggcttcgg cgaccaatga
     7501 agaggcgatt aacttttatc atcgccattt tgacgttgcc cgcgtggtgc tgccgcgcgt
     7561 gttgtcgatt catcaggtga aacagctggc acgggttact cctgtcccgc tggaagtgtt
     7621 tgctttcggc agcctgtgca ttatgtcgga aggccgttgc tatctgtcgt cgtatctgac
     7681 gggtgagtcg cccaacactg tgggtgcgtg ttctccggcc cgtttcgtgc gctggcaaca
     7741 aacgccgcag gggctggaat cccgcctgaa cgaagtgctg atcgaccgtt atcaggacgg
     7801 cgaaaacgca ggttatccga cgctgtgtaa agggcgttat ctggtggacg gcgagcgcta
     7861 tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa ctgctgccgg agttaatggc
     7921 tgcgaatatt gcttcggtga aaattgaagg ccgccagcgt agcccggcgt atgtcagcca
     7981 ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag gccgatccgc aaaacttcgt
     8041 accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc gaaggcacgc aaaccaccct
     8101 tggcgcgtat caccgtaaat ggcagtgaga agagcaatga aatattcctt agggccagtg
     8161 ctgtggtact ggccaaaaga gacgctggaa gatttttatc agcaggccgc cgccagcagc
     8221 gccgacgtga tttatcttgg tgaagcggta tgcagcaagc gccgggcaac caaagttggc
     8281 gactgtctgg agatggcaaa atcgctcgcc ggaagtggca agcagattgt gctctccacg
     8341 ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga aacgctatgt tgagaacggt
     8401 gagtttctga ttgaagccag cgatctcggc gtggtgaata tgtgcgctga acgcaaactg
     8461 ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg tgacgctgaa aatattgctc
     8521 aaacagggca tgatgcgctg gtgtatgccg gtggagcttt cccgcgactg gctggtgaat
     8581 ctgcttaatc agtgcgatga gctgggcatt cgcaaccagt ttgaagtgga agtcctgagc
     8641 tacggtcatc taccgctggc ctactccgcc cgctgcttta ccgcgcgttc ggaagaccgc
     8701 ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga acgggcgcaa cgtgctgtcg
     8761 caggaaaacc aacaagtgtt tgtactcaat ggcattcaga ccatgagcgg ctacgtttac
     8821 aacctcggta acgagctggc atccatgcag ggattggttg atgtagtccg cctgtcaccg
     8881 cagggcactg acactttcgc gatgctcgac gccttccgcg ctaatgaaaa tggcgctgtg
     8941 ccactgccgt tgacggcaaa cagcgactgt aacggctact ggcggcggct ggcaggactg
     9001 gagctgcaag cctaagtaaa tagctcactt tgttaacaac tttaactatt ctttaatgca
     9061 gtattaaaga ttaatcggta acaaagtgag ctgttatgac tgataaaacc attgcgtttt
     9121 cgctactcga tctggccccc atacccgaag gctcttcagc gcgggaagcg ttctcccact
     9181 ctctcgatct cgcccgtctg gctgaaaagc gcggctatca tcgctactgg ctggcagaac
     9241 accacaatat gaccggcatt gccagtgctg caacatcggt attgattggc tatctggcgg
     9301 cgaataccac cacgctgcat ctggggtctg gcggcgtgat gttgcctaac cactcaccgt
     9361 tggtcattgc cgaacagttc ggcacactca atacgctcta tccggggcga atcgatttgg
     9421 ggctgggtcg tgctccgggt agtgaccaac ggacaatgat ggcgctacgt cgtcatatga
     9481 gcggcgatat tgataatttc ccccgcgatg tcgcggagct ggtggactgg tttgacgccc
     9541 gcgatcccaa tccgcatgtg cgcccggtac caggctatgg cgagaaaatc cccgtgtggt
     9601 tgttaggctc cagcctttac agtgcacaac tggcggcgca gcttggtctg ccatttgcgt
     9661 ttgcctcaca cttcgcgccg gatatgttgt tccaggcgct gcatctttat cgcagtaact
     9721 tcaaaccgtc ggctcggctg gaaaaaccat acgcgatggt gtgcatcaat attatcgccg
     9781 ccgacagcaa ccgcgacgct gaatttctgt ttacctcaat gcagcaagcc tttgtgaagc
     9841 tgcgccgtgg cgaaaccggg caactgccgc cgccgattca aaatatggat cagttctggt
     9901 caccgtctga gcagtatggc gtgcagcagg cgctgagtat gtcgttggta ggcgataaag
     9961 cgaaagtgcg tcatggtttg cagtcgatcc tgcgcgaaac cgacgctgat gagattatgg
    10021 ttaacgggca gattttcgac caccaggcgc gcctgcattc gtttgagctg gcgatggatg
    10081 ttaaggaaga gttgttggga tagtgtgtct taacgcggga agccttatcc gagctggcaa
    10141 cgctgtccta cgtaggcctg ataagcgaag cgcatcaggc attgtgtagg cagcagaaat
    10201 gtcggataag gcaccgctga ttactgatac accggcagta aattaaagct cgataaaata
    10261 tgcaccagtg cgttgccgac gccaaacacc agaatcagcg caatcatcgg cttgccaccc
    10321 cagacgcgga atttcgggct gccaaagcgt ttacgcgatg cgcgggccag cagtgccgga
    10381 acaattgccg cccagatggt agccgctaaa ccagcataac caatggcata caggaatccg
    10441 ttcgggaaca gcagaccacc cacaacaggc ggggcaaagg tcagcaatgc cgttttcaag
    10501 cggcccacag ccgagtcgtc gaaaccaaac agatctgcca gatagtcaaa caaacccagc
    10561 gttacgccaa ggaacgaact cgctaccgca aagtttgaga acacgaccag cagaaggtcc
    10621 aggctacggc tgttcagcac gccgcttaat gcttgtacca gcacatcaat attaccgccc
    10681 ttctcggcga tgccgataaa ctccggacga gggatgttgc ccattgtcgc cagcaaccag
    10741 atggtataca gcgccagtgc catcagcgta ccgtacacca ggcatttcac gatggttttc
    10801 ggatctttgc cgtaatactt catcaggctt ggcacgttac cgtgataacc aaacgacgcc
    10861 aggcagaacg gcagggtcat cagcaaatac ggcgcataag atgcattgct ttcggcaacg
    10921 ttgaacaatg tcgcaggctg cacatgcccc agcaggctac cgaaggtgag gaagaaggta
    10981 atgactttcg cccccagcac aatcgccgtc atgcggctga cggctttagt actcaaccac
    11041 accacaaacg ctaccagcag tgcaaaacca aaacccgccg cccgtgccgg gacgtttagc
    11101 gacatctctg cgaaggtgtg atgcagaatc gaaccactgg cagaaatata ggcataggtc
    11161 aggatataga gcacaaaggc aatggaaatg ccgttgacca cgttccagcc tttgcccagc
    11221 aaatctttgg tgatggtgtc aaaactcgaa ccgattcggt aattcaggtt agcttccaga
    11281 atcatcaagc cggaatgcag catacagaac caggtaaaga tcagcgctgc cattgaccag
    11341 aaaaaccacg ccccggacat gaccactggc agagaaaaca tccctgcgcc aataatggtg
    11401 ccgccgataa tcaccacgcc gccaagcagc gacggtgacg tttgggtggt ggttagtgtt
    11461 gccatgaggg cttctctcca gtgaaaaata gtgcgactgc gttgttatgc attgcactgt
    11521 accagtacat gagtacaaaa gacagaaaaa aagccccgat ggttaaaatc ggggctgtat
    11581 atattatttt acagattgtg ttcgctgttc agcgatgatt acgcatcacc accgaaacga
    11641 cgacgaccgg tagagtcatc acgacgcggt gcgcggcctt cacgacgttc gccgctaaaa
    11701 cgacgaccat caccacgacc accttcacgg cattcaccgc tgaagttacg accgccttca
    11761 cgacgctcgc caccgaaacc acgaccaccg ccacgacgct caccgccagt atgcggctgt
    11821 gcatcgccca gtaactgcat gttcatcggc ttgttgagaa tgcgagtgcg cgtaaagtgt
    11881 tgcagcactt cacccggcat acctttcggc agttcgatgg tggagtgaga agcaaacagc
    11941 ttgatgttac cgatgtaacg gctgctgatg tcgccttcgt tagcaatcgc gccaacgata
    12001 tgacgaactt caacaccatc atcgcggccc acttcaatgc ggtacagctg catatcgcca
    12061 acatcacgac gttcacgacg cggacgatct tcacggtcgc cacgcgggcc acggtcgtta
    12121 cgatcgcgcg gaccacggtc atcacggtca cggaattcac gtttcggacg catcggcgca
    12181 tctggcggaa caatcagagt acgttcaccc tgtgccattt tcagcagtgc cgcagccaga
    12241 gtttcgagat ccagctcttc accttctgca gtcggctgaa ttttgctcag cagtgcgcgg
    12301 tattgatcca gatcgctgct ttccagctgc tgctgtactt tagcggcgaa tttttccaga
    12361 cggcgtttgc ccagcagttc tgcgttcggc agttctactt ccggaatagt cagcttcata
    12421 gtacgttcaa tgttgcgcag cagacgacgc tcgcggttct caacgaacag cagcgcgcgg
    12481 ccagcacgac ccgcacgacc ggtacgaccg atacggtgaa cgtaagactc agaatccatc
    12541 gggatatcgt agttaaccac caggctgata cgctcaacgt ccaggccacg ggccgcaacg
    12601 tcggtcgcaa tcaggatgtc cagacgaccg tctttaaggc gttccagtgt ctgctcacgc
    12661 agcgcctggt tcatgtcacc gttcagcgcg gcgctattgt agccgttacg ctcaagagct
    12721 tcagccactt ccagagtcgc gtttttggta cgaacgaaga taatcgccgc atcaaaatct
    12781 tccgcttcca ggaaacgtac cagtgcttcg tttttacgca taccccacac agtccagtag
    12841 ctctggctga tgtcaggacg ggtagtcacg ctggactgaa tacgcacttc ctgcggctct
    12901 ttcataaagc ggcgggtaat gcgacgaatc gcttccggca tggttgcaga gaacagagcg
    12961 gtctgatgac cttccgggat ctgcgccata atggtttcaa cgtcttcgat gaagcccatg
    13021 cgcagcattt cgtcagcttc atccagaacc agaccgctca gtttagagag gtccagagtg
    13081 ccacgtttca ggtggtccag cagacggccc ggagtaccga caacgatctg cggcccctga
    13141 cgcagggcgc gtaattgcac gtcataacgc tggccgccgt acagggcaac cacatttacg
    13201 ccgcgcatgt gtttagagaa atccgtcatt gcttcagcaa cctgtaccgc cagttcgcgg
    13261 gtcggtgcca gcaccagaat ctgtggtgct ttcagctcag gatcaagatt ctgcagcaga
    13321 ggtaaagaga atgctgcagt ttttccgctc cccgtctggg ccatacccag aacgtcgcgg
    13381 ccattcagca gatgtggaat acactctgcc tgaattggag atggtttttc gtaacccaga
    13441 tcgttaaggg cttcaaggat aggagccttc aggcccagat ctgcaaaagt ggtttcgaat
    13501 tcagccatgt agtacgtgtg cctcaaaatt aatggcggcc agtctacata actcatcatg
    13561 aaattgatca gcaattttca ttgaaaagtg tgaaccggct caaagtaggt gtattaacga
    13621 acaacaacgc cctcacccgt taaggtgatg gcaatcaaaa aagattacgg gctgatgtgt
    13681 acgtcagcta ttgctggtcc gattctgcca ggtcatcttg gtcctggccc aggagcgata
    13741 attccaacaa tgcgtatcgg tgctcaacaa agttatgaac gttgttggca accgccagtt
    13801 tgaacagtgc cgtggcgctg tccaaatccc ccagacttag gtagtactta cctaaataga
    13861 agttggtttc actgagatgc tcagcgagcg aggtgttatc cgttgcgtcc gccttgagcc
    13921 tttccattaa cgtttgttcg ctaatgttgc ccaggtagaa ctcgacaatg ttccatcccc
    13981 actgttcctt atccgatttt tcgaagtgct gtttcaacac ttctttagcc tgcttctcat
    14041 cgagcttctg ctcggcgaga taaagccaca gactacggaa aggatcattg ggatcgtctt
    14101 gataaaacgc cagcagatca tcttgcgcta acttgtcacg accgccgtaa tataatgcga
    14161 tcccgcgatt caagtgcgcg tagttgtaag ttggatcaag ctcaagtaca gaatcaaacg
    14221 cttcataggc agcatcaaaa ttgcctgcct gcgttaaata tatgcctaag taattgaata
    14281 cttcaggcat atccggtcgg attgccagcg cttgcgaaaa atcgttacgc gctaatgccc
    14341 tcagaccgag actatcatac aacactccgc gctcatataa aagctgtgcg cgttcgtcat
    14401 cggttaaagc ccgactggca aggatttgtt ccatacgtgc cagaatcact tcctgctgta
    14461 aagtcggttg caatggtacc gcgaggactt cacttttacg ccaggaagta ttactgcatc
    14521 ctgcaagcgt aagtgctgtc gcaacgaaac accagcgcaa aaaaggcttc atttcccact
    14581 cccgaagacc acggttgaat gaacgtcctg ttcccggttg ctaacaaggc gtcctgcccg
    14641 gttaaaagcc ccccgccgca gcggagggca aatggcaacc ttactcgccc tgttcagcag
    14701 ccggagcttc cggtgctgca gcaggttgag actgctcagt cgcttcttta atgctcagac
    14761 ggatacggcc ctggcgatca acttccagaa ctttcaccgg tacttcctga cccatctgca
    14821 ggtaatcggt cactttctca acgcgtttgt cagcgatttg agagatgtgt accagacctt
    14881 ctttaccgcc gccgatggca acaaatgcgc caaagtcaac gatacgggtc actttaccag
    14941 tgtagacgca gcccacttcg atttctgcag tgatctcttc gatacgacga atagcatgtt
    15001 tcgctttctc gccgtcggtc gctgcaatct tcacagtacc gtcatcttcg atttcgatgg
    15061 tggtgccagt ttcttcggtc agggcacgga taacagagcc gcctttaccg ataacgtctt
    15121 tgatcttgtc cgggttgatc ttgatggtat ggatacgcgg tgcgaactca gagatatcgc
    15181 cacgcggcgc gttgatcgcc tgttccatta cgcccaggat atgcagacgc gcacctttag
    15241 cctggttcag cgcaacctgc atgatctctt tggtgatacc ttcaattttg atatccatct
    15301 gcaatgcaga gataccctcg cgggaacccg caactttgaa gtccatatcg cccaggtgat
    15361 cttcgtcgcc caaaatgtca gacagtacaa cgtagttgtc gccttctttc accagaccca
    15421 ttgcgatacc cgcaacggca gctttgatcg gcacacctgc gtccatcagc gccagagacg
    15481 cgccgcacac ggaagccata gaagaggaac cgttggattc agtgatttca gacacaacac
    15541 gtacggtgta cgggaatttg tccatatccg gcatgactgc cagcacgccg cgcttcgcca
    15601 gacgaccgtg accaatttca cgacgcttcg gagaaccgac catgccggtt tcgcctacgg
    15661 agtacggagg gaagttgtag tggaacagga aggtatcggt acgttcgccc atcagttcat
    15721 caagaacctg cgcgtcacga gcagtaccca gcgttgcggt aaccagcgcc tgcgtttcac
    15781 cacgggtgaa cagcgcagaa ccgtgagtac gcggcagcac gccagtacgc acatccagac
    15841 cacggatcat atctttttca cgaccgtcga tacgcggttc gcctgccagt acgcggctac
    15901 gaacaacgtt tttctcgatc gcgtgcagaa tttcacccag ttcgttttcg tccagggttt
    15961 cgtcttcagc aagcagcgtc gcgatggttt cagatttgat gacatcaacc tgcgcataac
    16021 gctcttgttt gtcggtgatg cggtaagcat cgctcaggcg agcttcagcc agtgcagcaa
    16081 cgcgcgcgtt cagcgcttcg tttaccggct ccggctgcca gtcccaacgt ggtttaccgg
    16141 cttctttcac cagttcattg atgttctgaa taacaacctg ctgttgttca tgaccgaaca
    16201 ctactgcgcc cagcatctgg tcttcgctca gcagttcagc ttcagattca accatcagta
    16261 cagctgcttc agtaccggca acaaccagat ccagtttgct ctctttcagc tcgtcctgag
    16321 tcgggttcag tacgtactgg tcattgatgt aacctacgcg agcagcacca atcggaccat
    16381 tgaacggaat accagacaga gacagtgctg cggaagcacc aatcatcgcg acgatatccg
    16441 ggttaacttg cgggttaaca gaaaccacgg tggcgataac ctgaacttcg ttgacgaagc
    16501 cttccgggaa cagcgggcga atcgggcggt caatcagacg cgcgatcagg gtttcgcctt
    16561 cgcttgggcg gccttcacga cggaagaagc tacccgggat acgaccagca gcgtaggtac
    16621 gctcctgata gttaacggtc agtgggaaga agtcctgacc tggtttggct tttttctggc
    16681 caacaacggt aacaaatacc gcggtgtcat ccatgctaac cataacagcg gcagtagcct
    16741 gacgagccat catgccggtt tccagagtca cggtgtgttg gccgtactgg aatttacgaa
    16801 cgatcggatt aagcaatgta atatcctttc tctttcttag acagtacctt acggcactgg
    16861 tgttaatacc cgatcttctg cgcatcctcg cgactaatga caaccctaac ccagctctat
    16921 gtgggtaaag cctctcatta gccgcgcgaa cctctgcaac ggaagatcat tcatagcaac
    16981 aatacattag tttccagtga attgctgccg tcagcttgaa aaaaggggcc actcaggccc
    17041 ccttttctga aactcgcaag aattagcgac gcagacccag gcgctcgatg agctgggtgt
    17101 aacgtgctac gtctttacgt ttcaggtagt cgagcagttt acgacgctga gaaaccatgc
    17161 gcagcagacc acgacggctg tggtgatctt ttttgtgctc tgcaaagtgg ccctgcaggt
    17221 ggttgatctg tgcagtcagc agtgctacct gaacttcagt agaaccggtg tcgtttgcgt
    17281 cacgaccaaa ctcagaaacg attttagctg ttgcttcagt acttagagac attttaaaac
    17341 tccaaagtat atagaatgaa aggacgccga tctctaattc agcgatccca gtgtacgtta
    17401 cgcaaagtgt taaacaattt acgcgacgtt aagcggcagt attctactcg tagcgacctg
    17461 ttatcgcaag acggttaaca ttacgccggg tattcaacca ccaggcgacg aggcgcaacg
    17521 cggccttcat cgtcaatttc gcccataccg ataaatttgc cgttctcacc ttctgtaacg
    17581 cgaaccagtc cttccagcgg cgcaccggat gtacgaactg ggttaccatt tttgaagtaa
    17641 acagaagacg ttaacggaag attcaccacc gggtagtccg aagctggact gtccattggc
    17701 atcagtaatg gatcaagtaa ctccgcagcc ggaatatcct gctgttcggc ttgttcgacc
    17761 agttcacaca gatgttccag ggtcaccatc cgttcaaccg gatatttact taccgccaga
    17821 cgacgcaggt aaataacatg cgcgccacaa ccgagttttt caccaaggtc atcaatgatg
    17881 gtgcggatat aagtgccttt tgagcagtga atttccagct ccagctcatt gccttcgtgg
    17941 cgaataaaca gcaattcata aacggtaatc gggcgcgctt cacgcggtac ttcaatgccc
    18001 tgacgcgcat attcgtacag ttttttaccc tgatatttca gtgctgaata catcgaaggg
    18061 atctgttcga tatcgccacg gaaagtatcc agcgccgccg ccagttgctc tgcgctaaag
    18121 gttaccggac gttcttcaac gatttgtccg tcggcatcag aagtatcggt acgctgtcca
    18181 agacgcgcaa tgacccgata gcgtttgtcg gagtccagca gatactggga aaacttcgtc
    18241 gcttccccga ggcaaattgg caacatgcca gttgccagcg agtccagcgc accggtatgc
    18301 ccggcacggt tggcgttata aatccgtttc actttttgca gcgcatcatt gctggacata
    18361 ccctgcggtt tatccagcaa caaaacgccg ttaatgtcgc gaccgcgacg acgaggacga
    18421 ctcattagtc ctccttgctg tcgtccgggt taacacgacg ttcttcgtca tgtttgacca
    18481 cgctggtcac caggtttgac atgcgcatcc cttcaaccag agagttgtcg tagaagaagg
    18541 tcagttccgg cacgatacgc aggcgcatcg ctttccccag caggctgcgg atgaaaccag
    18601 aagcttcttg caacgctttg atgcccgctt taaccgcgtc ttcatctttg tcgttgagga
    18661 acgtcacata tactttggca tacgccaggt cgcgagacat ttcgacaccg gaaacggtgg
    18721 tcatcatgcc caggcgagga tctttaattt cacgctgcag gatgagagcg atctcttttt
    18781 gcatttcctg cgctacgcgc tgcgggcgac caaattcttt cgccataata aattctcctg
    18841 acaaaaaagg ggctgttagc ccctttttaa aattaatttc aggtggaagg gctgttcacg
    18901 ttggcctgat aagacgcgcc agcgtcacat caggcaatcc atgccggatg cggcgtaaac
    18961 gccttatccc gcatggaacc ctaaaaacct taagcgatgg tacgttggat ctcgatgatt
    19021 tcgaatactt cgatcacatc gccagtgcgg acgtcgttgt agttcttaac gccgatacca
    19081 cattccatac cgttacggac ttcgttaacg tcatctttga agcggcgcag ggactccagc
    19141 tcgccttcgt agataaccac gttgtcacgc agaacgcgga tcgggttgtg acgtttaacc
    19201 acaccttcgg taaccataca gcctgcgatg gcaccaaatt tcggcgattt gaacacgtca
    19261 cgaacttccg ccagaccgat aatctgctgt ttcagttccg gagacagcat accgctcatc
    19321 gccgctttca cttcgtcaat caggttatag atgacggagt agtaacgcag atccaggctt
    19381 tccgcttcaa tcactttacg tgcagaggca tcagcacgta cgttgaagcc aaccaggatg
    19441 gcgttggacg ccgcagccag ggtggcgtcg gtttcggtga taccacctac gccagaaccg
    19501 atgatcttca ctttaacttc gtcagtagac agtttcagca aggagtcgga gatcgcttcc
    19561 acagaaccct gtacgtctgc cttcaggacg atgttcactt cgtgaacttc gccttcggtc
    19621 atgttggcga acatgttctc gagtttagat ttctgctgac gcgccagttt aacttcgcgg
    19681 aatttaccct gacgatacag tgcaacttca cgcgctttct tctcgtcacg tacaacggta
    19741 acttcatcac ccgcagccgg tacgccggac aggccgagga tttccaccgg aatggacgga
    19801 cccgcttcca gcacttcctg acccagttcg ttacgcatcg cacgaacacg accgtattcg
    19861 aagccacaca gaacgatatc gcccttgtgc agagtacctt cacgtaccag aacggtagca
    19921 accggaccac gacctttatc gaggaaggat tcgataaccg caccgctcgc cataccttta
    19981 cgtaccgctt tcagctccag aacttccgcc tgcagcagga tagcgtccag cagttcatcg
    20041 ataccagtac ccgctttcgc agacacatgt acgaactggc tttcaccgcc ccactcttcc
    20101 ggcaggatgc cgtactggga gagttcgttc ttaacgcgat ccggatcggc ttctggttta
    20161 tcgatcttgt tcactgcaac gaccaccgga acctgcgccg ctttcgcgtg ctggattgct
    20221 tcgatcgtct gcggcattac gccgtcgtcg gcagcaacaa ccaggactac gatgtccgtt
    20281 gcctgcgcac cacgagcacg cattgaagta aacgcggcgt gccccggggt gtccaggaag
    20341 gtgatcatgc cgttttcagt ttcaacgtgg tatgcaccaa tgtgctgggt aatgccgccc
    20401 gcttcgccag aggccacttt cgttgaacga atgtagtcca gcagagaggt tttaccgtgg
    20461 tcaacgtgac ccatgatggt cacaaccggc gcgcgcggtt cagccgcagc acccgtgtca
    20521 cggtcgctca ttaccgcctc ttccagctcg ttttcacgac gcaggataac tttatggccc
    20581 atctcttcag caaccagctg tgcggtttcc tgatcgataa cctggttgat ggttgccatt
    20641 gcgcccagtt tcatcatcgc tttgatgacc tgagagcctt taaccgccat cttgttcgcc
    20701 agttcgccaa cggtgatagt ttcgccgatc acaacgtcac ggttaacggc ctgagcaggc
    20761 ttctggaagc cttgctgcag cgaagaacct ttacgttttc cgcctttacc gccacgtact
    20821 gctgcgcgtg cttcttcacg atcagctttt gattcagcgt gtttgttgcc tttcttcgga
    20881 cgcgctgctt tcgcgttacg accacggcca cggccgcctt cgacttcacg atcgctttcg
    20941 tcttctgcct ggcgagcatg ttgagaagta gtgacgtgat aatcgctgga atcttcagtc
    21001 ggttccgcgt tatcagtcca tttgttttct tccgccatac gacgtgcttc ttcagcaacg
    21061 cgacgtgctt cttcttcgag tttacgacgc gcttcttctt cagctttacg cttgagctct
    21121 gcagcttcct gctcacggcg ggctttttca gcctgggcgt ttttagtcat atcgtcttgt
    21181 tgattgctca ctttgtcttt ttccgcagct tcacgtttcg cttgttcagc agcttcacgc
    21241 ttagcttgtt ctgcggcctc acgttcagct ttttgttgcg cctcgcgttt agccgattct
    21301 tctgcctcac gacgggcttg ctcttccgct tcacgctgcg cttgctcttc cgctgcaagg
    21361 cgttcagcct cttgcggatc gcgtttcaca aaggtgcgtt tcttgcggac ttcgatttgt
    21421 accgatttgc tttttccacc ggtaccaaga atgttaaggg tgctacgtgt tttacgttgc
    21481 agcgtcaatt tgtccgggcc tgaatttttc tgattcaggt ggtcaatcaa agtctgtttc
    21541 tcttgtgcag acacagagtc gtcagcagac ttccggatac ctgcatcagc aaattgctgt
    21601 accaggcgtt ccacggaggt ctgtcgctct gcggccagcg ttttaatcgt tacatctgtc
    21661 atgctgttcc ttcctgctac agtttattac gcttcgtcac cgaaccagca aatattacgg
    21721 gcagccataa tcagtgctcc ggctttttcg tcggtcaacc cttcgatatc agccagatca
    21781 tcaatgccct gttcggcgag atcttccagc gtacaaacgc cacgggcggc cagtttgaat
    21841 gccaaatcac gatctacccc ttcaaggttc agcagatcgt cagccggttt gttatcaccg
    21901 aggctttctt cctgggcctg tgcaatggtg gccagtgcat ttttagcacg ctcgcgcagt
    21961 gcttcaacgg tcggctcatc aaggccttcg atttccaaca gctctttcat cggcacatag
    22021 gccagttctt ccagcgtcga gaaaccttct tctaccagaa cagtcgcgaa gtcttcgtcg
    22081 atgtcgagat atttggtgaa ggtgtcgatc gctgcgtgcg cttccgcctg atgcttagcc
    22141 tgcaggtcgt caacggtcat tacgttgagt tcccaaccgc tcagctgcga agccagacgc
    22201 acgttctgac cgttacggcc aatcgcctgc gccaggttac cggcttcaac ggcgatatcc
    22261 atggtgtgtt tatcttcatc caccacgata gaagcaacgt ctgccggtgc cattgcgtta
    22321 atcacaaact gcgccgggtt atcatcccac agaacgatat cgatacgctc gccgcccagt
    22381 tcagtagaaa ccgcctgaac acgcgcgcca cgcataccta cgcaagcacc taccggatcg
    22441 atacgtttat cgttggtttt caccgcgatt ttcgcacgag aacccgggtc gcgagccgct
    22501 gctttaattt caatcacttc ttcgccgatt tccggcactt caatacggaa cagttcgatc
    22561 agcatttccg gcttggaacg agtgacgaac agttgcgcgc cacgcgcttc cgggcgaacg
    22621 gaataaagca cgccacgaac gcggtcgcca gggcggaagt tttcacgcgg cagcatatct
    22681 tcgcgcagga tcacggcttc agcgttgttg cccagatcca gagagatgtt gtcgcggttt
    22741 acttttttca ccacgccggt gatgatttca ccttcgtgtt cacggaactg atcaaccacc
    22801 atcgcacgtt cggcttcacg cactttctgc acgataacct gttttgccgt ctgggtggtg
    22861 atacggtcaa aggtaacaga ctcaatctga tcttcaacgt aatcgcccag gttcaggctt
    22921 tcatcttcat aacgtgcggc ttcaagggtg atttccttgg tcggctgggt gacttcatca
    22981 acaactaacc agcgacggaa ggtgtcaaaa tcaccgcttt tgcgatcgat ctgtacacgg
    23041 acgtcgatct cttgctcata ttttttcttt gttgctgtcg ccagcgcgct ttccaatgct
    23101 tcgaaaatct tctcgcgagg tagcgccttt tcattggata cggcttcaac tacagccaaa
    23161 atttctttgt tcatcgcggg cttttcacct catccagact attaaaagtg gggaaccagg
    23221 ttcgccttct ggatattact cagcgcgaac acttcatctt taccttcgac ggtaactgtg
    23281 atcatttcac cgtctaccgc tttgataacg ccctgccatt tacgacggtt ttgtaccgcc
    23341 atacggagaa ccagagtcac ctcttctccg acaaaacggg cgtagtgttc agccgtgaac
    23401 agtgggcgat cgagacccgg tgaggagact tccaggttat aagcaacggt gatgggatct
    23461 tcaacatcca gcacagcact tacctggtgg ctcacatcag cacaatcatc aacattgatg
    23521 ccatcttcac tatcaatata gatgcgcagt gtggatgtgc gaccgcgaat aaattcgatg
    23581 cctaccagtt caaaacccag ggcctcaact ggcgcagtaa tcatctctgt taatttttgc
    23641 tctaatgtgg acaagcccac ccccaagaca taaaaaaagg gcctaaagcc cagttattct
    23701 gtagtcagat aacaaaaaac cccgataaat cggggcttta tataactgaa ccctataacc
    23761 gcaactgcgg tctggagcac tttccagaag gattttttca aatcccacta cgaaggccga
    23821 agtcttcaca gtatatttga aaaaggactc taagggaaa
//
LOCUS       DADLSA010000050        23143 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.050, whole genome shotgun sequence.
ACCESSION   DADLSA010000050 DADLSA010000000
VERSION     DADLSA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.050"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(260..877)
                     /gene="tdk"
                     /locus_tag="JN691_002006"
     CDS             complement(260..877)
                     /gene="tdk"
                     /locus_tag="JN691_002006"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAY7243460.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1482..1895
                     /gene="hns"
                     /locus_tag="JN691_002007"
     CDS             1482..1895
                     /gene="hns"
                     /locus_tag="JN691_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAY7243461.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2039..2947)
                     /gene="galU"
                     /locus_tag="JN691_002008"
     CDS             complement(2039..2947)
                     /gene="galU"
                     /locus_tag="JN691_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAY7243462.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3149..4162)
                     /gene="rssB"
                     /locus_tag="JN691_002009"
     CDS             complement(3149..4162)
                     /gene="rssB"
                     /locus_tag="JN691_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAY7243463.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4254..5159)
                     /gene="rssA"
                     /locus_tag="JN691_002010"
     CDS             complement(4254..5159)
                     /gene="rssA"
                     /locus_tag="JN691_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAY7243464.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5272..5730
                     /locus_tag="JN691_002011"
     CDS             5272..5730
                     /locus_tag="JN691_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAY7243465.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5780..6622
                     /gene="purU"
                     /locus_tag="JN691_002012"
     CDS             5780..6622
                     /gene="purU"
                     /locus_tag="JN691_002012"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAY7243466.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6782..6866
                     /locus_tag="JN691_002013"
     tRNA            6782..6866
                     /locus_tag="JN691_002013"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6816..6818,aa:Tyr,seq:gta)
     gene            6913..7044
                     /locus_tag="JN691_002014"
     ncRNA           6913..7044
                     /ncRNA_class="other"
                     /locus_tag="JN691_002014"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7228..7905)
                     /gene="narI"
                     /locus_tag="JN691_002015"
     CDS             complement(7228..7905)
                     /gene="narI"
                     /locus_tag="JN691_002015"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAY7243467.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7905..8615)
                     /gene="narJ"
                     /locus_tag="JN691_002016"
     CDS             complement(7905..8615)
                     /gene="narJ"
                     /locus_tag="JN691_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAY7243468.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDVVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8612..10150)
                     /gene="narH"
                     /locus_tag="JN691_002017"
     CDS             complement(8612..10150)
                     /gene="narH"
                     /locus_tag="JN691_002017"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY7243469.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10147..13890)
                     /locus_tag="JN691_002018"
     CDS             complement(10147..13890)
                     /locus_tag="JN691_002018"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY7243470.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPIADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNSVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYVH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14283..15674)
                     /gene="narK"
                     /locus_tag="JN691_002019"
     CDS             complement(14283..15674)
                     /gene="narK"
                     /locus_tag="JN691_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAY7243471.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16013..17809
                     /gene="narX"
                     /locus_tag="JN691_002020"
     CDS             16013..17809
                     /gene="narX"
                     /locus_tag="JN691_002020"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAY7243472.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17802..18452
                     /gene="narL"
                     /locus_tag="JN691_002021"
     CDS             17802..18452
                     /gene="narL"
                     /locus_tag="JN691_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAY7243473.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18453..19847)
                     /gene="ychO"
                     /locus_tag="JN691_002022"
     CDS             complement(18453..19847)
                     /gene="ychO"
                     /locus_tag="JN691_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAY7243474.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20032..20385
                     /gene="ychN"
                     /locus_tag="JN691_002023"
     CDS             20032..20385
                     /gene="ychN"
                     /locus_tag="JN691_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAY7243475.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20429..21124)
                     /locus_tag="JN691_002024"
     CDS             complement(20429..21124)
                     /locus_tag="JN691_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY7243476.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21282..21512)
                     /gene="chaB"
                     /locus_tag="JN691_002025"
     CDS             complement(21282..21512)
                     /gene="chaB"
                     /locus_tag="JN691_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAY7243477.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21782..22882
                     /gene="chaA"
                     /locus_tag="JN691_002026"
     CDS             21782..22882
                     /gene="chaA"
                     /locus_tag="JN691_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAY7243478.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYSVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         5797 a         6038 c         5570 g         5738 t
ORIGIN      
        1 ccttctgggg ccgttttttt gtttacccaa agcaacccga cagcattagc cttcatcata
       61 tttgcgacga tgtataacgc ctaaacacag ggatattgta ctttacaggt cacaagtcaa
      121 cgtcggtgct taagagccct gtgaggcgta tagcggcgtt aaaaaactgc cgagaagggt
      181 atatagcccg gaagaagtgc gtaaaacgaa ctgacaggat aaaagtgccc tgctcaccct
      241 gtcagtaaag aaattcttat taatcgtggc gatgcctttc ctgaatagcc gttaatgagc
      301 cgacttgtaa cgcctcttta tagtgtttac ggcatacaga aacgtatcgt tcattaccac
      361 caattaccac ctgctcacct tcgttataag gtctgcctgc ttgatcaaga cgcagcacca
      421 tgcttgcttt acggccacaa aaacagatgg tttttaattc aaccagtttg tcggaccatg
      481 ccagtaagta ttggctgcca ataaataatt cacctcgaaa atcggtacgt aaaccataac
      541 aaagtacggg tatatcgagt tgatcgacaa cctccgataa ttcatatact tgttgtctgg
      601 ttaaaaactg gcattcatca accagtacgc aatgaattgc ctgctgttca tgttccgcac
      661 gaatctcatc aaataatgat gaattttggt taaataattt tgcaggcgat gacaaaccta
      721 tacgcgaact gactttcccg gcaccaaagc gatcatcaat ttctgccgta tatacgacag
      781 tgcgcatgcc gcgttcctgg taattgtatg aagattgcaa caatgctgta gacttacccg
      841 cattcattgc ggaatagtag aaatatagct gtgccatcag ccacaggccc tcaatgatat
      901 gaaataaagt tggctggagt ttatcataat tcgtcagttt ttcagctcaa cagtatgcac
      961 agaagtgggt atatgcgttc tcccttacga agccttgcat aatccttctg aactatcatt
     1021 acaactgcct cgcttgttat aagcgggtaa atgactgctg gtaactattc acaatcttta
     1081 acctgttgcg caagtaatag ccctctgttg acctccaaga gatagtgcaa tactaagtcc
     1141 atgctcttat tgcgactgtt ctacttttca tcattcgctt aatagggaat tctcgtaaac
     1201 acaactaata cagaagactg aaaggtcgtc agcctacgat aatctcccca taaaatgtga
     1261 catgaatcag gaagttttaa cctcacgtgc tgcgaaatca tcggtgtaaa tagggctata
     1321 tgccgcgtct tttctggcta attttatgaa aagatattta ttggcggcac aaaataaaga
     1381 acaattttga attccttaca ttcctggcta ttgcacaact gaatttaagg ctctattatt
     1441 acctcaacaa accaccccaa tataagtttg agattactac aatgagcgaa gcacttaaaa
     1501 ttctgaacaa catccgtact cttcgtgcgc aggcaagaga atgtacactt gaaacgctgg
     1561 aagaaatgct ggaaaaatta gaagttgttg ttaacgaacg tcgcgaagaa gaaagcgcgg
     1621 ctgctgctga agttgaagag cgcactcgta agctgcagca atatcgcgaa atgctgatcg
     1681 ctgacggtat tgacccgaac gaactgctga atagccttgc tgccgttaaa tctggcacca
     1741 aagctaaacg tgctcagcgt ccggcaaaat atagctacgt tgacgaaaac ggcgaaacta
     1801 aaacctggac tggccaaggc cgtactccag ctgtaatcaa aaaagcaatg gatgagcaag
     1861 gtaaatccct cgacgatttc ctgatcaagc aataatcttt tgtagattgc acttgcttaa
     1921 aatcccgcca gcggcgggat tttttattgt ccggtttaag acaatttaat aagaataagc
     1981 tataaaaaaa cggcgtcgat tgctcaacgc cgtttcgtgg ataacaccga tacggatgtt
     2041 acttcttaat gcccatctct tcttcaagcc aggctttaaa ttccgtgcca agggtgttat
     2101 gacgaatacc gtattcaacg aaggcctgca tgtaacctaa tttattaccg cagtcatggc
     2161 tcttcccttt catatgatag gcttccaccg tttctttttc gatcagcata tcaattgcgt
     2221 cggtgagctg aatttcatca ccagctcccg gaggggtttt tgccagcaac ggccaaatat
     2281 ccgcgctaag tacgtaacga cccacaatag cgagattaga cggcgcaaca tccgctttcg
     2341 gtttttctac cacaccaacc atcggtacgc tttcacccgg cgctaattca acgcctttgc
     2401 aatccacaac gccatatgcg gtcacatcag caaccggttc aaccatgatc tggctatgac
     2461 ccgtttcatc aaagcggcgg atcatctctg ctaggttatc ctgtgacaaa tcggattcat
     2521 attcatccag aataacatca ggcaaaataa cagctaccgg ttcatcaccc actaccgggt
     2581 gagcacacaa taccgcgtgt cccaggcctt tcgccagacc ctgacgaact tgcataatag
     2641 tcacgtgcgg tggacaaata gactgcactt catcaagcag ttgacgtttt acacgttttt
     2701 ccagcattgc ttccagttca aaactggtat caaagtggtt ttcaatagag tttttagatg
     2761 agtgtgtaac cagcacaatt tcagtaatgc cagccgcaat acattcattc acgacgtatt
     2821 gaattaatgg cttatcgaca agtggcagca tctctttcgg gatggctttc gtcgccggca
     2881 acatcctggt tcctaatccc gcaacgggga taacggcttt tttgactttc gtattaatgg
     2941 cagccattta aattctcctg gactgttcgt gttttgaacg tgttcatatt tctgtatcgc
     3001 atcccagtat atcagcaccc cgaaggggct aagttctgaa aaggaggcga gatagcataa
     3061 ttaagacaat tacttatacg aatggcgacg atagtaccac cagccgacat tagcaggtaa
     3121 tgcaaattta gcccgcgtta tcgtttgctc attctgcaga caacatcaag cgcagtcgac
     3181 caccggttcc ccatatttgg cattgccagg catcgcatcg ctggctcaat tgattcaaat
     3241 aagcgttacc taaagtgcct aacggaacac cattactgat ttgcacctgg tgttcgccgg
     3301 tatttaacgt cgcattcaga cccgcagaaa ccagaatgag atttttcagt tcgcgatgat
     3361 aatagccaac taatagcgga aactgccccg gcagattggc ctgacgaagt aaatggttta
     3421 cctgcttcaa taacgcgccc aactctggca accgttgatt ttggtgtgca agctgttcct
     3481 gtaataatcc gttaaacaat gcgcgtaata acaaggcagc aagtacgcca ttatgtccag
     3541 ctcgggtgac atcaaggcaa taaaatgcca gatcgttttc cgaaagtgcg gcaatatcaa
     3601 gcaccaggcc gggtttgtcc gcggcaacca attgacggta attaacccgg caatgggaaa
     3661 tcacctgctg aaccggcggt tgtagttcct gtaataattt cgccgctgcg gcagggttat
     3721 caaccattgc atcccagtcg cgaaaaagcc tttcctcttc ctcaacgcgc gaattaaaca
     3781 tgctgggata gagacaggca aaaaccatct cgcgcaagcg attcagatct ttaactggtt
     3841 tcagcaaaac atcttcaacg cccagacgta acgctttggc aatatctgcc atattttcag
     3901 tggcagatat caccagaact ggggtctggt cgcctctgtt acgtatatgc tccagcagtt
     3961 taagcccgtt cattcgtggc atcgcgatat cacatatcat caggtctgga gtgaaacctc
     4021 ccagcaactc aagggcatcc accccatcag ccgccagtac cgttgtcgct cccaatgagg
     4081 aaaaccatga atccagaagc gagcgaaata cctgctcatc ttcaacaatg agaatctgtt
     4141 ttccgaccaa tggctgcgtc atgttctctc ccctgactgg ctttactcaa tagtggcatg
     4201 ctattgccac ctgcgcctgt cagaattaac ttaagtgtag ataaaaattc tggtcaaatg
     4261 ttggtgcgta ccaacggcaa aagttcgtcc attttccttt ccactgccag ctgtccggcc
     4321 gcaatggcag cgtgcgcgcg atggaaatca agcgtagata tttgcgggca aacaggttga
     4381 atcagaatat cgggcggatc acctgccatg cggttccttt taaggcggtt ctccaacacc
     4441 tggatagacg tggtcataat ctctgttgcc gttggcgctg tcacagcccg acgtgtcgtt
     4501 atgctgccta acctttcttt cagacgcgca tgccacggta gagaatcacc attctcgcta
     4561 ttttcttcac tgacattaaa ggagagcaga tcttgttgca tcaaatgagc atcgtgctgc
     4621 aggtcgaccg ctatcacaat atcagccccc aatgcacgcg taagagaaat aggaattggg
     4681 ttaactactg ccccatcaac cagccagtag ccgttatgtg caacgggtgc catgagtcct
     4741 ggaatactgc atgatgcgcg aatagcaaga tggagatcgc cttcagtaaa ccataattca
     4801 cgccccgtac ttaaattggt ggcaacagcc gcaaagcgac gggaacaatt ttcgatctct
     4861 gtttccggca ttatttcgcg atattgattg aagacacgct cgccgcgcag taacccaccg
     4921 cgctgccagg agaggtccat caggcgtaaa acgtcccaat aactgaaaga ggttacccaa
     4981 tcttccagcg cagataatcg atcgcatgca taggccgcgc ccaccagcga accaattgaa
     5041 catcctgcaa cgatatcaat ttcaataccc acttttttta gcgcattaat aacgccaata
     5101 tgcgaccaac ctctcgccgc gccagatccc agcgccagcc ctatttttat ctttctcatt
     5161 attcccgcaa gattcccctg gaatgcaatc gtagccacat cgctactgct cggttaacat
     5221 agtgctactc aggcggttaa tacgccgtta ttgtttccag ggagattatt tgtgtctcag
     5281 ctttgtccct gtggtagtgc tgtcgagtat agcctatgtt gccaccctta tgtgtctggt
     5341 gaaaaggttg cacctgatcc agaacatctc atgcgttcgc gttactgcgc ttttgtgatg
     5401 caagacgctg attatttaat aaagacctgg catccctctt gtggagcagc ggcgttacgt
     5461 gccgaattga tgaccggatt tgcgcatact gaatggttgg gattgaccgt atttgaacat
     5521 tgttggcagg acgccgacaa tatcggattt gtcagttttg ttgctcgatt taccgaaggt
     5581 ggcaaaacag gtgcaataat tgaacgttcg cgatttttga aagaaaacgg tcaatggtac
     5641 tatattgacg gtacacgtcc gcagtttggt cgcaacgatc cctgcccttg tggttcaggt
     5701 aaaaaattta aaaagtgctg cggccaataa tggttgacgg tacggtttag caaacactct
     5761 caacaaggtt tttccagcaa tgcattcact ccaacgtaaa gttctgcgta ctatttgtcc
     5821 ggaccaaaaa ggtctgatcg cacgtattac caatatttgc tacaagcacg agttaaatat
     5881 cgtacagaac aatgaatttg ttgatcaccg taccgggcgc ttttttatgc gcacggaact
     5941 ggaagggatt tttaatgatt ccaccctgct ggcggatctc gatagcgcat tgccagaagg
     6001 ctccgtgcgt gagctgaatc ctgccggtcg tcgccggata gtgattctgg tcactaaaga
     6061 agcgcattgc cttggcgatt tgttgatgaa agccaattac ggcggtctgg atgtcgaaat
     6121 cgcggcagtt attggtaacc acgatacttt acgttctctg gttgagcgtt ttgatattcc
     6181 gtttgagctg gtaagccatg aagggttaag ccgcaacgag cacgatcaaa agatggcgga
     6241 cgccattgat gcttatcaac ctgactatgt ggtgctggcg aagtatatgc gggtattaac
     6301 gccggaattt gtggcacgct tcccgaataa aatcatcaat attcaccatt cattcctgcc
     6361 tgcgtttatc ggcgcacgtc cttatcacca ggcctatgaa cgtggtgtga agattattgg
     6421 cgcaaccgct cactatgtga atgacaatct ggacgaaggc ccaatcatca tgcaggacgt
     6481 tattcatgtc gatcatacct acacagctga agatatgatg cgcgcaggtc gtgacgtcga
     6541 gaaaaacgtc ttaagtcgtg cgctatacaa agtactggcg caacgcgtct ttgtttacgg
     6601 taatcggacg attattcttt aatcgccagc aaaaataact ggttaccttt aatccgttac
     6661 ggatgaaaat tacgcaacca gttcattttt ctcaacgtaa cactttacag cggcgcgtca
     6721 tttgatatga tgcgccccgc ttcccgataa gggagcaggc cagtaaaaag cattaccccg
     6781 tggtggggtt cccgagcggc caaagggagc agactgtaaa tctgccgtca tcgacttcga
     6841 aggttcgaat ccttccccca ccaccatcac tttcaaaagc cccggaattc tcaaacgaat
     6901 ctgcaatcaa atattctgcc caagcgggga aggatgagaa gcttcgacca aggttcgact
     6961 tgagcgccag cgagagagcg ttgccgcagg caacgacccg aagggcgaag cgcgcagcgc
     7021 tgagtaatcc ttcccccacc accatcgctt ccttgaataa tcaaaacctg ttactccgag
     7081 ctaattttgc ccgatggcac cacgtttacc gggcctacaa tatcctcccc tgtaggtcgg
     7141 ataagcgaag cgcatccgac aactcacccc gcaccgtgcc cataaaaaaa ccccgccgaa
     7201 gcagggtcta tgtgaactaa aattcgttta gtgacgagcg cgcaccagct ggtacttacg
     7261 tgtcagatac tccaccggta cgctccagat gtgtaccaga cgcgagaacg ggaacagcag
     7321 gaataacgtc atccccagca ccaggtgcag acggaagata aacgccacac catcgaggtg
     7381 ttgagaagcg ccaccgtgga aggtcaccac cgactgcgcc cagccaacca gtttcatcat
     7441 ctcgctaccg tccatatgct gagcggagaa cggaatggtc agcaggccca gcgcgcactg
     7501 gataacgagc agcgacagga tcaggatatc cgctccggta gtggttgcac gcacgcgtgg
     7561 gctgaacata cgacgtttca gcaaaagcac gccgccaatc agacacagca cgccgctggc
     7621 accaccagca aacatcgcca ttttctgttt cacttcaatc ggcagccagg cttcatacat
     7681 ccagtgcggc gtcaacatac cgaagaagtg accgacaaaa atccccagaa taccgatatg
     7741 gaacaggttc gacgccaggt tcatcccttt gcgatccagc atctggctgg acgccgcgcg
     7801 ccaggtgtac tgcccgtagt cataacgcag ccagctacca atcaggaaga ccgccccggc
     7861 tatgtacggg tagatatcaa agaagaacat attcaggaat tgcattagtg ctgtcctccg
     7921 gtggtgatat tcagatattg cggcgcgacg gcaccggcaa agcgacgctg atgagcagtg
     7981 attgctgaat caccgcagcc tttgtcagca aagaatttaa cctgctcttc ttcccagaca
     8041 acatccagcg cctgcggcgt atcatcgcgc gcttcgtcgg caattttttc cgccactttg
     8101 tcgctgtcga tagcggtatt ggccaatttc agcagcagat caaacatcac ggcataacgg
     8161 ctttcacgct gttgcagacg cgcgctcagc aatgccagaa tcggcgcgat atctttcaaa
     8221 ccttccacgg cttcgctttg cggcagctgc gacaggtact ccagatacag cggcagatgg
     8281 tccggtaatt cgcggctgtt taactgcaag ccgtgctgct cgtactgcgc cagcaggtcc
     8341 accatcgcct ggccgcggtc acgggattca ccgtgaacat gttcaaatag caacagtgac
     8401 gtggcgcggc cacggtcgaa cagttcgctg tactgggcct gggcatcgag cggatccatc
     8461 gccgttaaat cgcgcaggaa aatgcccagc gcatgggcat cttctttcga cagatttttc
     8521 gacgcagcaa tcgcctcaaa catctcttgt tgatgctgcc ataaggcagc atccggatat
     8581 tcaaggagac gcgatacaat cacgagttcg atcatggatg cggctccgtt ttgctggtca
     8641 catcgatggc atcgatacga cggctgttga acagattgaa tttggtatct gagccgtggc
     8701 agccatcacc aaaggtaaag ccgcagccat ttttctccgg gaaggcttcc cgtgccagtt
     8761 cacgatgact actcggcacc acaaagcgat cttcgtagtt agcaatcgcc agataacggt
     8821 acatctcctg tgcctgggct tcggtcagac cgacctcttc cagcgcacgg gtatcaactt
     8881 taccgtcaac agtttcagca cgtttgtaat gacgcatcgc cagcatacgt ttcagcgcac
     8941 gcagtaccgg tttggtatca ccggcggtca gcagattcgc cagatactgt accggaatac
     9001 gcaagctttc gacgtctggc agaattccgt tactgcccag ctcgcccgcg tctgctgcag
     9061 actgaatcgg agacagaggc ggcacgtacc agaccatcgg cagtgtgcga tattctggat
     9121 gcagcggcag cgccagtttc cattccatcg ccattttata aaccggcgac tgctgtgcag
     9181 cttcaataac gctcagcgga ataccgtctt taatcgcctg ctcgatgact ttcggatcgt
     9241 tcggatcgag gaacacgtcc agctgacgct ggtagagatc tttctcgttc tcggtgctgg
     9301 ctgcacgttc aatcgcgtcg gcatcgtaca gcagcacgcc aagataacgg atacgaccga
     9361 cacaggtttc tgagcacacg gtcggctgac ccgcttcaat acgcggatag cagaagatgc
     9421 acttctcaga cttaccgctc ttccagttga agtagatttt tttgtacggg catccggtga
     9481 tgcacatacg ccagccacgg catttatcct ggtcgatcag gacgatgcca tcttcttcac
     9541 gcttgtaaat cgcaccgctc gggcaggtcg ccacacatgc cgggttcagg cagtgttcgc
     9601 acaggcgcgg caaatacatc ataaaggtgt tttcgaactg gctatacatc gccttctgga
     9661 tgttgtcgaa gttcttgtct ttcgccagtt tatcaaactc accgcccagg tcatcttccc
     9721 agttcggccc tttttcgatt ttcgccatcc gttccccggt aatcagcgaa cgcggacggg
     9781 caatcggctg cgatttgctg ccttccggcg cggtatgcag gttctgataa tcaaaatcga
     9841 acggctcgta ataatcgtcg atccccggca gatgcgggtt agcaaagatt ttacccagca
     9901 gcatggctcg gttacccatg cgcggttgca gtttgccatt gattttacgg atccagccgc
     9961 ctttgtattt ttcctggttt tcccagtcag tcgggaagcc ctggcccggc ttggtttcca
    10021 cgttgttgaa ccacgcgtat tccacgcctt cacggctggt ccagacgttt ttacaggtaa
    10081 ctgaacaggt gtggcagccg atgcacttat cgagattcag caccatgccg acttgtgaac
    10141 gaattttcat tttacgctct cctgtacctg gtcattgcct tcgccatcta accagtcaat
    10201 gttcttcatc ttacgcacta caacaaactc atcgcggtta gaacctacgg tgccgtagta
    10261 gttaaagccg tatgccagat ggacatagcc gccgatcata tgcgtcggtt tcggcgtgat
    10321 acgggtgacc gagttatgga taccaccacg ctgttgggta atttccgaac caggcaggtt
    10381 aacgatacgt tcctgcgcgt ggtacatcat ggtcatccct gccggaacac gctggctgac
    10441 aaccgcacgg gcagtcagtg caccgttgct gttgaagact tcaatccagt cgttatcggc
    10501 gatacccaga tctttggcat cggcttcact caaccagacc accggaccac cgcgacctaa
    10561 agtcagcatc agcaggttgt cgctgtaggt ggagtggata ccccacttct ggtgcggcgt
    10621 caggaagttg agcgcttttt ccgggttgcc gttggatttc tggcctatca cttctttcac
    10681 cgaacgggtg tcgatcggcg gacgataaac cagcaggctt tcaccgaaat cacgcatcca
    10741 ctggtgatcc tgatacagtt gctgacgacc agagagcgta cgccatggga tcagctcgtg
    10801 aacgttggtg taaccggcgt tgtaagaaac gtgttcatct tccagaccag accaggtcgg
    10861 gctggagata attttgcgcg gctgtgcctg aatatcgcgg aagcggatct tctcgtcttc
    10921 tttattcagc gccagatgcg tatggtcacg accggtaaat tcgctcaggg cagcccaggc
    10981 tttcacggct acctgaccgt tggtttccgg tgccagcgtc aggatcatct ctgccgcatc
    11041 aattgcggtg ttcagcatcg gctggccttt tgccggacct tccgctttgg tgtagttgag
    11101 cttacgcagc agatccatct cgctctgggt gttccaggca atccctttac cgccgttacc
    11161 gattttctcc atcagcgggc cgatagaggt aaagcgttcg taagttgccg gataatcgcg
    11221 ctctacgacc ataatgtgcg gtgcagtttt gcctgggatc aggtcgcatt cgcctttttt
    11281 ccagtctttc acatccagcg gctgcgccag ttcagcggca gagtcgtgct ggataggcag
    11341 cgtgacgatg tcggtttctt tacccagatg gccgacgcac acttcggaga atttcttcgc
    11401 gatggctttg tagatttccc agtcgctttt cgcttcccag gccggatcga ccgccgcaga
    11461 cagcgggtga ataaacggat gcatatccga agtattcatg tcgtcttttt cgtaccaggt
    11521 cgccgtcggc aaaatgatgt cggaatagag acaggtgctc gacagacgga agtccagcgt
    11581 aaccaccaga tccagcttgc cttccagacc attgtcctgc cagtccactt cttccggctt
    11641 cacgccgccc tgttgcccca gatctttacc ctggataccg tgctccgtcc ccagcaggta
    11701 cttgagcata aactcatgac ctttaccgga agaaccgagc aggttagaac gccagatgaa
    11761 caggttgcgc gggtggtttt taccgttttc tggttgttct gccgcaaaac ggatggaacc
    11821 ctctttcagg gatttcaccg tatagtccac cgaattcatc ccggcttttt ccgcttcgcc
    11881 agcgatagtc agcgggttag tgcctaactg cggtgcagac ggcagccagc ccatgcgttc
    11941 cgcacggacg ttaaagtcga tcaagtgtcc ggtatagcgg gatttgtccg ctatcggtga
    12001 cagcaactct tccgccgtga cggtttcata acgccactgg ctggagtggt tatagaaata
    12061 agaagtgctg ttcatgtgac gcgccggacg ctgccagtca agggcaaacg ccagcggctg
    12121 ccagccggtt tgcggacgca gtttttcctg acctacatag tgcgcccagc cgcccccgct
    12181 ctgaccgaca cagccgcaga aaatcagcat gttgatcaga ccacgatagt tcatatcgag
    12241 gtgataccag tggttcagcc ccgcaccgac gataatcatc gaacgaccgt gcgttttatc
    12301 agcgttatcg gcaaattcac gggcgatgcg aataatctgg ctgcgagaaa cgccggtaat
    12361 ctgctcggcc caggccgggg tataagcttt cacatcgtca tagctggttg cacagttaac
    12421 gtcgttcagg ccacgttcca gaccgtagtt tgccagcgtc agatcataaa cggtggtcac
    12481 cagggcggtg ctgccatcag ccagttgcag gcgtttcacc ggcagtttgt gcagcagcac
    12541 gttttccagt tccactttgt tgaagtgttc cgtgccgtcg ccaccaaagt acgggaagcc
    12601 tacctcagcg atctcatcct ggctacccag caggctcagt tgcagctcgg tttcttcgcc
    12661 agttttgccg tcgcgctgct caagattcca tttgcccttc tcgccccagc ggaagccaat
    12721 agaaccgttc ggcgcaacca tttcgccatt ggtattaaag gcgacagttt tccattccgg
    12781 attgttttcc tggcccagcg catcaaccag atcagcagcg cgcagcatac gacctgcagc
    12841 gtagtaaccg tcgcgttctt ccagcatcac cagcatcggc atgtcggtgt agcgacgcac
    12901 atagtcggtg aaatactggc ttgggttgtc gaggtggaat tcacgcagca ttacgtggcc
    12961 catcgccagc gccattgccg catcggtgcc ctgtttcggt gccagccaca gatcgcacag
    13021 tttggcgatt tcagcgtagt ctggtgtgac ggcaacagtt ttggtgcctt tgtaacgcac
    13081 ttcagtaaag aagtgagcat ccggggtacg cgtctgcggc acgtttgaac cccaggcgat
    13141 gatgtagctg gagttatacc agtcagcaga ttccggtacg tcagtttgct cgccccaggt
    13201 ttgcggagac gcaggaggca ggtcgcagta ccagtcgtag aagcttaagc aagtaccgcc
    13261 aatcagcgag agatagcgtg cacccgatgc gtaagaaacc atcgacattg ccggaattgg
    13321 cgagaaacca gcaacacgat ccgggccgta gtttttgatg gtgtaaacgt tagatgcggc
    13381 gatcagttcg ttcacctcct gccaagaaga acgaacaaat ccaccgcgtc cacgcgcctg
    13441 cttaaagctt ttcgctttat cggcgtcttc aatgatagaa gcccatgcct caaccggatc
    13501 gctatgcagc gccttcgctt cacgccacat tttcatcagg cgtttgcgca tcatcgggta
    13561 tttcaggcgg ttggcactgt aaagatacca ggagtagctg gcaccgcgcg ggcagccgcg
    13621 aggttcatgg tttggcagat ccggacgggt acgcggatag tcagtctgct gggtttccca
    13681 ggtgaccaga ccgtttttga cgtagatttt ccagctgcag gagccggtgc agtttacccc
    13741 gtgggtagag cggacgattt tgtcatgctg ccaacgctgg cgatatccat cctcccagtc
    13801 acggttggta ttgagaagct ggccatgccc atcggcaaag gtttcaccct tctgtttgaa
    13861 gtagcgaaac cggtccagga atttactcat cggttttctc ctgtgggagc ctgtcggccc
    13921 tctgataaat cgacattgct aatgtttaag ggcaaggtaa cgctctgaaa cagcgtggga
    13981 attgataacg atcaagagtg atggggatga aaaataaagt aaattcctaa gatacccctt
    14041 taggataatt aacagttaca gcataacatg ctgtaaatta agatgtttta tacctgtagt
    14101 tatttaaact attaagattt ttatgcaaat gggtattaag gagtattccc catgggtaac
    14161 atattaatgg ctcttaatga aatccggcga aataagccat ataacactaa tatgttgtaa
    14221 ctaaatgacg cataaaaaaa gcgcggcatc atgccgcgca aaggataatc aaatattaag
    14281 atttattttt tagaatgccg accatatacc gcccaggtaa tcactacgca ggcgatatag
    14341 aaaatcaaaa atactttcat tgcacctact ggcgaacccg ttaatgccag cgagctacca
    14401 aacgctttcg ggataaagaa gccaccaatc gcgccaatcg cagagatgaa acccagcgcc
    14461 gctgccgtgt cggttgccgc ttcacgcatc gcacgttcgt cagaaccccc ttctgctttc
    14521 acgcgatcca ttgtcagttt acggaagatc actgaaatca tctggaaagt ggaaccacta
    14581 cccagcccag ctgtcaggaa cagcgccagg aagaccgcga agaacgccat gaagcttccg
    14641 ccctgcccgt cagtcggtaa ggtcaggaac agcaggccgc tgaaaatcgc catcagaata
    14701 aagttcacca gcgtgacacg agttccgccc agacggtcag ataatgcacc acctgcagaa
    14761 cgcgccagcg caccaataaa cggcccgaag aaagcgtatt gcagaatctg aacatccggg
    14821 aactgcgttt ttgacagcat cgcaaagccc gcggagaagc cgatgaagga gccgaaggtt
    14881 gccagataca gcaggctcat aatccacaga tgaccccgtt tgagtaccgg caactgctcc
    14941 ttgatggagg ctttcgaggt agcaagatcg ttcatgccaa accacgccgc aatggtgaag
    15001 atggcaagga acggcaccca tatccaggac gcattcgcca gatacagctc agtcccatcc
    15061 ggctgtttga cgccctggct accaaatact gcgaaaatcg acagtgatac caccagcgga
    15121 gcaaccaact gcatgacgct gacgcccatg tttcccagac caccattcag acccagcgcg
    15181 ccaccctgct tctgtttcgg aaagaagaag ctgatgtttg ccatactgga tgcgaagttc
    15241 gcgccagcaa agccgcatag cagagagatg atgatgaaga cgctataagg cgtggaggta
    15301 tcctgcacgg caaaacccag ccagacgcaa ggaataatca gaataccggt gctgaacgcc
    15361 gtccagcgac gaccaccgaa gataggaacc ataaaggagt atggaacacg taataacgcg
    15421 ccagaaaccg aaggcagcgc agtcaacata aatagctgat cggtcgtaaa attaaaaccg
    15481 actttcggta agttcaccgc aacagcgctg aacaacatcc atacgcaaaa cgccagcagc
    15541 agacagggaa cggaaatcca caggttgcgg ctggcaatac gttgaccgcg ttgttgccag
    15601 aacgcaggat cttccggtcg ccaatctgtt atgacagctc cagtagccct ttcggaggca
    15661 gatgagtgac tcatagacac ctctgatact cgtttcgatg tctgccacct tagtgtctgg
    15721 agctaaaggc aatttgatgt aaatcaaacg ataagataac tttatctatc attgatattt
    15781 atcattaccc atagtgagta cagtgatttc ataaaaatta tgagattttt cacggtgctg
    15841 tagaatccct acccttaccg atgtaaagcg actaaccaca cggcaaataa ggagtaactc
    15901 tttccgggta tgggtatact tcagccaata gccgagaata ctgccattcc agaatatatc
    15961 gtcacattca ttaaggttat tgctcattta aagcctgaag gaaggggttt acatgcttaa
    16021 acgttgtctc tctccgctca ccttggttaa tcaggttgcg cttattgtgt tgctttctac
    16081 tgctattgga ctggcaggga tggcggtttc tggctggctg gtgcaaggcg ttcagggcag
    16141 cgcccatgcg atcaacaaag cgggatcgct gcgcatgcaa agttaccgtc tgttggcggc
    16201 agtgccatta agcgagaaag acaagccctt aattaaagag atggaacaaa cggcatttag
    16261 cgccgagttg actcgagcag cagaacgaga cggacaactg gcgcaattac agggtttaca
    16321 agattactgg cgtaatgaac tgatccctgc gctgatgcgt gcacaaaacc gagaaacggt
    16381 gtcagcggat gtcagccagt ttgttgccgg gcttgatcag ctggtatctg gttttgaccg
    16441 caccacggaa atgcgtattg agacagtggt actggtccat cgggtaatgg cggtatttat
    16501 ggcactttta ctggtgttca ctattatctg gttgcgggcg cgactgctac aaccgtggcg
    16561 gcaactgctg gcaatggcga gtgccgtcag ccatcgcgat tttacccaac gcgcaaacat
    16621 cagcgggcgc aacgaaatgg cgatgcttgg aactgcgttg aacaatatgt ctgcagaact
    16681 ggccgaaagt tatgccgtac ttgagcagcg ggttcaggag aaaaccgccg ggctggagca
    16741 taaaaatcag atcctctctt ttttatggca ggctaaccgc cgtttgcatt cccgcgcccc
    16801 gctgtgtgaa cgcctgtcac cggtactcaa cggcttacag aatttaaccc tgctacgtga
    16861 tatcgaactg cgggtgtatg acactgatga tgaagagaat catcaggagt ttacctgcca
    16921 gccagatatg acttgtgatg ataaaggctg ccagctctgc ccgcgcggcg tattacccgt
    16981 tggtgatcgc ggcacgaccc tgaagtggcg gctggctgac tctcatacgc agtacggtat
    17041 tttgctggcg accctgccgc aggggcgtca tcttagccat gatcaacaac aactggtgga
    17101 taccctggtt gaacaactta ccgccacgct ggcgctggat cgccatcagg aacgtcagca
    17161 acagttgatc gtgatggaag agcgtgccac cattgcgcgc gaactgcatg attctattgc
    17221 ccaatctctc tcttgcatga agatgcaggt gagttgttta cagatgcagg gcgatgcgct
    17281 gccagaaagc agccgcgaac tgttaagtca gatccgtaac gaactgaatg catcctgggc
    17341 gcagttgcgt gaattgctca ccacattccg cttgcagctc accgagcctg gattacgtcc
    17401 ggcgctggaa gcgagttgcg aagagtacag cgccaaattt ggcttcccgg tgaagctgga
    17461 ttatcaattg ccgcctcgtc tggtgccttc acatcaggca atccacttgt tgcaaattgc
    17521 ccgtgaggca ttaagtaacg ccctcaaaca ttcgcaagcg agtgaggtcg tggtgacggt
    17581 ggcgcaaaac gataatcagg tcaaactgac cgtccaggat aacggctgcg gcgtgcctga
    17641 aaatgccatc cgcagcaatc actacggcat gataataatg cgcgaccgtg cgcaaagttt
    17701 acgaggcgat tgccgcgtcc gccgtcgtga atcaggtggc accgaagtgg tggtcacctt
    17761 tattcccgaa aaaactttca cagacgtcca aggagatacc catgagtaat caggaaccgg
    17821 ctactatcct gctgattgac gatcacccga tgctgcgaac tggcgtaaaa cagcttatca
    17881 gtatggcacc agatatcacc gtggttggcg aagcgagtaa tggcgaacag ggtattgaac
    17941 tggcggaatc tcttgatccc gatctgatcc tgttagatct caatatgccc ggcatgaacg
    18001 gtctggaaac gctggataaa ctgcgcgaaa agtccctctc agggcgcatt gtggtattca
    18061 gcgtctctaa ccatgaagaa gatgtggtca ccgcactgaa acgcggcgcg gatggctatc
    18121 tgttaaaaga tatggaaccg gaagatctgc tgaaagcatt gcatcaggca gctgctggcg
    18181 aaatggtatt aagcgaagca ttaacgcctg ttctggccgc cagcttgcgc gctaaccgtg
    18241 ccactactga gcgcgatgtt aaccagttaa ccccacgcga gcgcgatatt ctcaagctga
    18301 ttgcccaggg tttgccgaac aagatgattg cccgccgcct ggatatcacc gaaagcacag
    18361 taaaagtgca cgtcaagcac atgctgaaga aaatgaagct caagtctcgc gtggaagcag
    18421 cggtatgggt gcatcaggag cgcattttct gattacggtt cccagcgcag ttcgtcgttt
    18481 gacaatgcat cgaacggttc gacaagcgtt agcgtgatct cattggagga gacacgctgc
    18541 ccctggttat cttccaccac cacagacaat cgccagtgat tgcttgcccc ttccccgttc
    18601 tgccagtcag gcatgatcag cgtccagccc tccgcgctgt tggcttgtgc gcccggcgtc
    18661 aaacttaata tctgcgtatc gccctgccaa atcagttgcc gaataccgta acgactgcgg
    18721 atttgtaatt tcagcggcac tgtttcgcca ggttttagat cccacggcgg tgtcgccaga
    18781 aacaccgtta acgtttttcg ctgtcggtac tcaagagtcg gtagattatt tcgctgcgga
    18841 ttgtcatagc gactaccacg taacgactga ctttcggcaa cctcgcccgc agaaagttgt
    18901 tttttgagcg gtacaccaaa gcggtaatta agattcagcc cgaggttatt ttgattttcg
    18961 ccactttccc cctgtttatg ctgggccgtc acagtgacta atggcacagg ggtgtaattt
    19021 aatcccagac tcaacgcgac gggattgtga taacccgtac cagagttaaa caaatcaaca
    19081 cgatcaccaa aatactgttc taggctgaca ctggtattga ggtgttgata gaacggcatg
    19141 cgcatccgag ctgtcaggtc gtacccgcgc gccatccgtt gttcctgcgt ggctgtctgt
    19201 tcatgccatg cagcaaacgg ctgataaaag tttgccgata atcgcaaata ttcgccccac
    19261 gcttcggcac caaagcccgc tcgctgaaga ttttcgtcca gcaagttgtc ataaaaagtg
    19321 ttataaccca ccagccagtt gccgcgcgcc cagcgttgcc caacgcccac attgctcacc
    19381 aacccattat cctgctgagt aagaccaagc tggctccagg tgagataacg atcattatct
    19441 tgtaacggca caaaccagct tccacgactg ccggtgaaat gtccttcgtt atccactttg
    19501 acgtcaacac tggcatttcc ccacggtgat agccaggact ctacgtgctg attaacctgt
    19561 tgactaagcg cgtcgcggac ttttcccaat gcgaaagctt ttgcctgctc gccagtatcc
    19621 agcccgttat cattcatact ggtttcgccg aaatctttca caatttcagc aaagtgtttt
    19681 tccccatcat gattttcggg agccatgcct aaatccggca gaccatcatt gttattatca
    19741 aagggatttg ccgctttttg ctcgaaggta gattgtgcgt tagctgtacc gcctgctaca
    19801 agcaagagta aataaaacgg gataatgcga ggaacgaaac ggctcaaaac gtcggtaact
    19861 tccggtaata atgaaaaacc atagccttaa agaataacga ttactggcaa cttttgtcgt
    19921 attttctcgc gctttcagga aaatccttaa aaatgtgacc gattggtcag acgctgcaga
    19981 agcagtaaga tagaagatga gtgtctaatt ccctgaatac aggagtaaat aatgcaaaaa
    20041 atcgtgatcg ttgccaatgg cgcaccttac gggagcgaat ccttgtttaa cagcttgcgg
    20101 ctggccattg cgttacgaga gcaggagagc aatctggatc tgcgtctgtt cctgatgtct
    20161 gatgcggtca cagccgggtt gcgcgggcaa aaaccagggg aaggctacaa cattcagcaa
    20221 atgctggaga tccttaccgc tcagaatgta ccggtgaaat tgtgcaaaac ctgtaccgac
    20281 gggcgcggga ttagcacact tcctctgatt gatggggtgg aaatcggtac tcttgtggaa
    20341 ctggcgcaat ggacgctgtc agccgataaa gtgctcacat tttaatgttt aagggggcgt
    20401 ctgacgcccc actatgaggg aagtttactc aggcgaatcc cggccttaac acaccatccg
    20461 gataattctc cgccagtagt ttttttaccg ataccagcaa atcattcagc ccatcgtcct
    20521 gcattcccag tttgataagc tcctgttcca gtgaaaacag gtattgtgca ttggttccca
    20581 gcggaccgct cgccgccgca atcaacggcg cgatgacctg agcgcgagtg tcagattcat
    20641 attctggatg tcgcgggtcc ataataaaca caatggcgtt tactgtgcgt ccatcatcaa
    20701 gatcaagctg acaccaggtt ggcagataac agccggtaat catctcgcgc ttccacaaca
    20761 gggttagttc ctgctccagc gtctcttctg gtagtcgata ggcgacgcct gtggtgcgtc
    20821 cgccctcttt cagtgcaagc atccgtcccg gctggtgcgc agtcccgcgc ccggcggtca
    20881 ggcgcaggca aaacgcgcga tgccatccaa ccagtgtacc ggtgcatgat tcggtaaact
    20941 ccagtgccgg attccacatc aacgatccat aaccgaagat ccacaccggt ccctcatcag
    21001 gtcgacaagc cagcgtcgcc gccagcgaag ccgcccgttg ttctgctgac cataagagtg
    21061 attcttcaat ggcaccaaat gccgttttac aatcggcatt catcaagaaa tcacgcgtta
    21121 tcaccttaca cctccgccac tgcatacttc cctgcgacaa ctttttggtg tcttccggac
    21181 atttttaccg ctcattttga aaccactaac gggacaatat gcaatccacg gaccacttgc
    21241 aaggcaacac cgcacgaata gctttaacta agctaccggt tttacgattt tttatgccat
    21301 ttatcatcat cccctttggc atattcatgc ttcacagcag cccaggccac tttatgcgcg
    21361 gtttcttcgc gactggcgtc atcacgccga tcttctttat ctttatattg atcccatgcg
    21421 ctgttgaacg cttctttata gatatcctgg gcatgagacg gtagaacgtg ctttacgctt
    21481 tccggcagat cgcttttcgt tttatacggc atcgttgact ccttttacaa agttgaacat
    21541 taagtgtggt caacaatatc tactcttgcc aaaacataag aattttcatg tcagactgtt
    21601 cttaagaaac gctaaaacaa acattgtcta aacgtaatac ttatttttat gccccaatga
    21661 gtaaaaaatc gacagcgggg taaaaattcg taaaatgtta ccgatatcca gacactttct
    21721 gttatttttc ctacctattt tttacatttt cgataaattc atctgcaaag ggataaccat
    21781 aatgtcaaat gctcaagagg cggtaaaaac ccgccacaag gagacttcgc ttattttccc
    21841 ggttctggcg ctggtagtgc tgttcctgtg gggaagtagc cagacactac cagtggtcat
    21901 tgccatcaat cttcttgcgc ttattggtat tttaagtagc gcctttagtg ttgtccgtca
    21961 tgcggacgta ttagcccatc gcctgggaga accttacggt tcgcttattc ttagcctttc
    22021 agtggttatt cttgaagtca gtttgatttc agctttaatg gcaaccggcg acgccgcgcc
    22081 aacgctaatg cgtgatacgc tctattcaat cattatgatt gttaccggtg ggctggttgg
    22141 cttttcatta ttgttgggcg gtcgtaagtt tgccacccaa tatatgaatc tgtttggtat
    22201 caagcagtat ttaattgcgc tgttccccct ggcgataatc gtactggtat ttccaatggc
    22261 tctgcctgcg gcgaattttt caaccggtca ggcgttactg gtagcattaa tttctgcggc
    22321 aatgtatagc gtatttttgc tgatccagac caaaacgcat caaagtttgt ttgtctacga
    22381 gcacgaagat gacagtgatg acgacgaccc gcaccacggt aaaccgtctg cccatagcag
    22441 cctgtggcat gctatctggt tgattatcca tctgattgcc gttattgcag tcaccaaaat
    22501 gaacgccagc tcgctggaga cattgctcga cagcatgaat gcccctgttg cctttactgg
    22561 cttcctggtg gcactgttga ttctgtcgcc ggaaggttta ggtgcgttaa aagcagtgtt
    22621 gaacaaccag gttcagcgcg cgatgaatct gttctttggt tcagtgttag caacaatttc
    22681 gcttaccgta cctgtcgtca cgctaattgc ctttatgacg ggtaacgaat tgcagtttgc
    22741 acttggtgcg ccagaaatgg tggtgatggt ggcctcttta gtgctgtgcc atatctcctt
    22801 ctccaccgga cgtactaacg tgctcaatgg cgcagcgcat ctggcactgt ttgccgccta
    22861 tttgatgacg atatttgcct gacagttata tctcctccga tgacggaaat cgggagagat
    22921 aagataaatt cctgcttatt gtatttcttc atactacctg gacagctcgt taatcagaat
    22981 tgcgagcctg taccacaacc tgcacgaaac cactgcaaca gctgttgaaa agccggaatg
    23041 tactctttat tgcaaacatt tcagggatga agtgaagatg aattgagacg tagtatccac
    23101 accaagccgg aattatccgg tgaggcgcaa tgttgcgggg gct
//
LOCUS       DADLSA010000051        22903 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.051, whole genome shotgun sequence.
ACCESSION   DADLSA010000051 DADLSA010000000
VERSION     DADLSA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.051"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            106..327
                     /locus_tag="JN691_002027"
     CDS             106..327
                     /locus_tag="JN691_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243479.1"
                     /translation="MTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQEGMHEQAIKIA
                     LRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(368..838)
                     /locus_tag="JN691_002028"
     CDS             complement(368..838)
                     /locus_tag="JN691_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY7243480.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(893..2095)
                     /locus_tag="JN691_002029"
     CDS             complement(893..2095)
                     /locus_tag="JN691_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HAY7243481.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPPEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGNEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(2182..2724)
                     /locus_tag="JN691_002030"
     CDS             complement(2182..2724)
                     /locus_tag="JN691_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243482.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            3003..3428
                     /gene="nudI"
                     /locus_tag="JN691_002031"
     CDS             3003..3428
                     /gene="nudI"
                     /locus_tag="JN691_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAY7243483.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(3467..4069)
                     /locus_tag="JN691_002032"
     CDS             complement(3467..4069)
                     /locus_tag="JN691_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY7243484.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSEGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            4377..5516
                     /gene="arnB"
                     /locus_tag="JN691_002033"
     CDS             4377..5516
                     /gene="arnB"
                     /locus_tag="JN691_002033"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAY7243485.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            5520..6488
                     /gene="arnC"
                     /locus_tag="JN691_002034"
     CDS             5520..6488
                     /gene="arnC"
                     /locus_tag="JN691_002034"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAY7243486.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSTHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            6488..8470
                     /gene="arnA"
                     /locus_tag="JN691_002035"
     CDS             6488..8470
                     /gene="arnA"
                     /locus_tag="JN691_002035"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HAY7243487.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHTARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            8467..9357
                     /locus_tag="JN691_002036"
     CDS             8467..9357
                     /locus_tag="JN691_002036"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAY7243488.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            9357..11009
                     /gene="arnT"
                     /locus_tag="JN691_002037"
     CDS             9357..11009
                     /gene="arnT"
                     /locus_tag="JN691_002037"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HAY7243489.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            11006..11341
                     /gene="arnE"
                     /locus_tag="JN691_002038"
     CDS             11006..11341
                     /gene="arnE"
                     /locus_tag="JN691_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAY7243490.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            11341..11727
                     /gene="arnF"
                     /locus_tag="JN691_002039"
     CDS             11341..11727
                     /gene="arnF"
                     /locus_tag="JN691_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAY7243491.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(11721..11993)
                     /gene="pmrD"
                     /locus_tag="JN691_002040"
     CDS             complement(11721..11993)
                     /gene="pmrD"
                     /locus_tag="JN691_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAY7243492.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(12098..13453)
                     /gene="menE"
                     /locus_tag="JN691_002041"
     CDS             complement(12098..13453)
                     /gene="menE"
                     /locus_tag="JN691_002041"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAY7243493.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(13450..14412)
                     /gene="menC"
                     /locus_tag="JN691_002042"
     CDS             complement(13450..14412)
                     /gene="menC"
                     /locus_tag="JN691_002042"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAY7243494.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(14412..15269)
                     /gene="menB"
                     /locus_tag="JN691_002043"
     CDS             complement(14412..15269)
                     /gene="menB"
                     /locus_tag="JN691_002043"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAY7243495.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(15284..16042)
                     /gene="menH"
                     /locus_tag="JN691_002044"
     CDS             complement(15284..16042)
                     /gene="menH"
                     /locus_tag="JN691_002044"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAY7243496.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(16039..17709)
                     /gene="menD"
                     /locus_tag="JN691_002045"
     CDS             complement(16039..17709)
                     /gene="menD"
                     /locus_tag="JN691_002045"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAY7243497.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFGSHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     VGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSCLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(17798..19093)
                     /gene="menF"
                     /locus_tag="JN691_002046"
     CDS             complement(17798..19093)
                     /gene="menF"
                     /locus_tag="JN691_002046"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAY7243498.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAALGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAVLRTLLQME"
     gene            complement(19172..19477)
                     /gene="elaB"
                     /locus_tag="JN691_002047"
     CDS             complement(19172..19477)
                     /gene="elaB"
                     /locus_tag="JN691_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAY7243499.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(19532..19993)
                     /locus_tag="JN691_002048"
     CDS             complement(19532..19993)
                     /locus_tag="JN691_002048"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7243500.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            20058..20975
                     /gene="rbn"
                     /locus_tag="JN691_002049"
     CDS             20058..20975
                     /gene="rbn"
                     /locus_tag="JN691_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAY7243501.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            21163..22382
                     /gene="elaD"
                     /locus_tag="JN691_002050"
                     /pseudo
     CDS             21163..22382
                     /gene="elaD"
                     /locus_tag="JN691_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         5672 a         6027 c         5589 g         5615 t
ORIGIN      
        1 ttgcgcatgc tggagcaggg ctttgaacgt gagattgtgc tggcggcaac ccaactcact
       61 gatgctgata ttccgaactg ttattaaaag gagagggaga gattgatgac tattgctgaa
      121 cggctacggc aggaaggaca tcaaattggc tggcaagaag gtaaattagt aggtttgcag
      181 caaggtaaat tagaaggttt gcaggaaggt atgcatgaac aagccattaa aattgctttg
      241 cgcatgctgg aacagggcat tgatcgtgac caggtgctcg cggccaccca gctaagcgaa
      301 gccgatctgg cagcgaataa ccactaatta acacaggccc acagccgatc cccatgggcc
      361 tttgatatca ataactacct tttatgcgtg gcccattttt gcctgattta aacatcgcca
      421 gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac gccaacagcg acaaagttcg
      481 ctccccacgc caggcattgc tgcgccatat caggagccac agccagaaaa ccagccgctt
      541 tacccgcagc acggatccgc cgaatgctgg tttcaataat tcgctgcact tccgggtgcc
      601 cggcgttatc cgggtagccc aacgacgcag aaagatccgc tggtccaata aacacgccat
      661 caatcccttc gacgtcgagg atctcgtcca ggttatccag tgccgtttta ctttccacct
      721 gcactaacag acaaagcgaa tcgttgactt gcgccatgta attctcaatg cgtccccagc
      781 gcgcagcccg tgccacactg gccccaacac cacgctcacc gtagggaggg ttggacaaat
      841 tttgccggag ggggaatgtg ccggtgcagt aaaccggcac agagtggcga aatcagacgg
      901 ataaagtcat ggactcaaca acctcaatcc agccatgctc actagcgatg tcctggccgt
      961 ttaaccaacg gcgtagcata ttcagtgcca tcattgcgca cacttcctga cggatagcca
     1021 ggctgtagcg tgtagtgctg aaacgcacac gcagagcgaa agtgccgtct ggcgtggcta
     1081 gcgcaaagtt gagatgctcg ttctcgaaac ccgaaacagc cagtgccagc ccggcaaaat
     1141 ggttggcccg ccgttctgta atccagtgcg cagtttgcgc cagggtttct tcctgtgaag
     1201 gaaccacttc acacgccagc aatggagcac ctgcgcgaga aagttgcaaa gccaataaac
     1261 caccggtgaa ttgctcgctc aacgtcaggc tgaactggcg gttttgcaat tcgcgactga
     1321 tctgcgcggg cagtccttcg ttgccttcga aaatcacgct ctgtccggca acacgtttaa
     1381 cgtccagcca cagtttttcc atcgcctgtt gctcgcttgc cggtccggtg agtttcagtt
     1441 cgatgatcgg cattgaggag cgatagccca ttgttacgcc cggcggcagt tgtagagtgt
     1501 ccaggctttg tgccagatcg ctttccgaac gaccaaaggt agttaaacgc agacaaaccg
     1561 gcggctgtgg taaagaaaaa cgctcgcgca ggcgcggcag gatttcgtgc tcgaccatca
     1621 ccttaaattc tggcggtacg ccgggagtaa agaacatcag gcaacgatta agctgcacgg
     1681 caaaaccaca ggcggtgcct accgggttat tgataaattc agcactggca ggcagctccg
     1741 cttgtttacg gttgctcggt gccattactc gtccacgttc gtggaaatag cgttccatct
     1801 ctttgagcca ggcttcatgc agcaccaggc cttcaccttt tgctgtcgca gcggcgagtg
     1861 cgcttaaatc atcgctggtc ggtcccagcc cgccgttaac gatcagcaca tcggcgtgct
     1921 gactacgttc gcgaagaatg gtgactaagt catcaaggtt atcccccacc gtattgcggc
     1981 gagataatgg cagcccctga tgaaagaaaa aatcggccag ccaggcagcg ttagtgtcaa
     2041 cgatttgccc gtataacact tcatccccgg tggatagcat ttccactttt aacatggtta
     2101 tctcctgctt ttgttatcga atcactataa cgcaaaggga gataaggcgt agtaagaaac
     2161 tgaatgccgc gccggaaggg atcagaaact ggcgttaacc ccaacatacg ggccgtcagc
     2221 cacggcgttg tcgcggttac cgtctttacc cgacagattc aggtagcgat aacccgcctc
     2281 aatactgact ggacgcataa tggtgtaacg cgcgccagca ttcgcttcct cataactttg
     2341 aataccgctg gagagcgaat ccggagagta gtaatactcg ccaaacaaac ggaagctgtt
     2401 gccaattttc cactgcaaac cacctcctac cgctgcggca taaccttcat cgccgtaatt
     2461 cgggttggtg tacacgcctt ttccgccaac ggtcgccatt aacggcccga gaggcagatt
     2521 caaccccagc cccacgcccg cgacgtcacc gtcgtcgtcg ttatgtgtcc agttaccgct
     2581 taaagccagg cccgtcgatt cagtcccaaa accgacacca atattggtgt attctttacc
     2641 cgcctggccg ctgatgctca ttgcattgac cgatgcagaa accaacagca ttccggccag
     2701 acctgctagc gcaatttttt tcattatccc ctttcccaca agcaattaat caaaattccc
     2761 aaagctgcgg gattgtactg cctggcacag aggattcaat gagcgaaaaa agggatctag
     2821 tcacttattg taactaaata cttcttaaat aattaaacgc cgaaaaaata aacacacttt
     2881 accgatacac acaaaaataa taataagtgc agttatcatt gcaaaagaga ttactgtcac
     2941 tttcacatat ttctgagtta ccgtattctc atggtttctt tttatttata aggaataccc
     3001 ctgtgcgaca acggactatt gtatgccctt tgattcaaaa tgatggtgct tatttgctgt
     3061 gcaaaatggc cgacgatcgc ggcgttttcc ccggtcaatg ggcgctttcg ggtggcggcg
     3121 tggagtctgg cgaacgaatt gaagaggcac tacgccgcga aattcgcgaa gaactgggag
     3181 aacagctgct tttgacagaa atcacgccgt ggaccttcag cgatgatatt cgcaccaaga
     3241 catatgcaga tggtcgcaag gaagagattt atatgattta cctgattttt gactgcgttt
     3301 ctgccaaccg agaagtgaaa ataaacgaag agtttcagga ctacgcgtgg gtaaaacctg
     3361 aagatctggt gcattatgat ttaaatgttg ccacccgaaa aacgttacgt ttgaaaggtc
     3421 ttctttaaca atgatgacag ccatcactgg ctgtcagatt gaaaatttag tggttaacga
     3481 attccccatc cagataaact ttgccttttt ccacatgcat gactaaacca tccagataat
     3541 caggtttaaa tgtcgcatca cgcttatttt tagcaatata tgtcaggcaa tgattatggg
     3601 tgaaaataac gatattttta tcaggcgctt tgctttgtaa gtccttaatt gcactataaa
     3661 tctcattacc gcactgaaga agtcgtttat ctaccgtcag ctttttaccc tcggaaaacc
     3721 aggtcgccga ctgaatggtc cggacggtat tactggaata aagatcgaaa tcagggatgt
     3781 cagcgctaaa agcgttgccc agttcacggg catcctgggt acctttaacc gtaatacctg
     3841 ttttatctga caagcattga ttggttgaac gatcgcaacg ttcagcatgg cgaaacaaaa
     3901 cgacaaccgg gtgctgctgt gccagtctgg ccaatgtttt attgtcgata cgtggcaaac
     3961 cattgctact ccaggcggca tgagtaccca gtccggcaat tgcagcgagc gccagtaaaa
     4021 tgatgaaata tttttttgac ttcaatgatg agcggcaaaa agctaacacg taaactccac
     4081 ctatagacaa gcgcaaccag acaattaccg tgaaattgag cgacatttct ggcgataatt
     4141 agcagttggt gtaatattaa aaatcctacg atgtcggcaa aatgcctcaa atttttgcca
     4201 aatgcaaagc ctaaataaga aaaaatataa aaatttcaat atttacgtct aatattagtt
     4261 tcttaaggtt aagttaatat tctatcctta aaatttcgct ccaaatggca aaatatacac
     4321 aacactcttt atagcaaata taagtggaca ggtattcaat ggcggaagga aaagcaatgt
     4381 cagaattttt gcctttttcg cgaccagcaa tgggcgtgga ggaactcgct gcagttaaag
     4441 aggttctcga atccggttgg atcacaaccg gtccgaagaa tcaggcgctt gagcaagcct
     4501 tttgccagtt gacgggaaat cagcatgcca tcgcggtcag ttcagccacc gccggaatgc
     4561 atatcacgct aatggcgttg gaaattggca agggcgatga agtgattacg ccttccctga
     4621 cctgggtttc aaccctcaat atgatttcct tgctgggtgc aacgccggta atggtggatg
     4681 tcgaccgcga tacgctgatg gtcacgcctg aagcgatcga atcagccatt acgccacgca
     4741 ctaaagccat cattccggtg cattatgccg gtgcgccagc agatattgac gccattcgcg
     4801 ccattggcga acgttacggc atcgcagtta tcgaagatgc tgcccatgcc gtcggtacgt
     4861 attacaaagg gcgacatatt ggcgcaaaag gtaccgctat tttttcattt catgccatta
     4921 aaaatattac ctgtgctgaa ggtggcctga ttgtaactga taatgaaaac cttgcccgcc
     4981 agctacggat gctgaaattt cacggtctgg gtgtcgatgc ctatgacaga caaacctggg
     5041 gccgtgcacc gcaggctgaa gtcttaacac cgggctataa gtacaatctg accgatatta
     5101 acgccgcgat tgccctgaca cagttagtca aattagagca cctcaacacc cgtcggcgcg
     5161 aaattgccca gcaatatcag caagcactgg cagctctccc ctttcagcca ttaagccttc
     5221 ccgcctggcc gcacgttcac gcctggcatc tgtttattat tcgtgtcgat gaacaacgtt
     5281 gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga aagaggcatt ggtaccgggt
     5341 tacatttccg cgccgctcac acacaaaaat attatcgcga gcgttttccc acgctgtcgt
     5401 taccgaatac cgaatggaat agcgaacgca tctgttcgtt gccgctgttc ccggatatga
     5461 ctaccgccga tgccgaccgc gtcatcacag cccttcagca actcgcagga caataagcca
     5521 tgtttgaaat ccaccctgtt aagaaagtct cggtggttat tcccgtttat aacgagcagg
     5581 aaagcttacc ggaattaatc aggcgcacca ccacagcctg tgaatcgttg gggaaagagt
     5641 atgagatcct gctgattgat gacggcagta gcgataattc cacgcatatg ctggtcgaag
     5701 cctcacaagc ggagaacagc catattgtgt ctattttgct taaccgcaat tacgggcaac
     5761 attcagcgat tatggcggga ttcagtcacg ttactggcga cttaattatt acccttgatg
     5821 ccgatctcca gaatccgcca gaagaaatcc cccgcctggt ggcaaaagcc gatgaaggtt
     5881 acgacgtggt agggactgta cgccagaacc gccaggacag ctggtttcgt aaaacagctt
     5941 cgaagatgat taaccggctt attcagcgca ccactggcaa agcgatgggt gactacggtt
     6001 gtatgctgcg cgcctatcgc cgtcatattg tcgatgcgat gttgcactgc catgaacgca
     6061 gcacctttat cccgattctg gcgaatatct tcgcccgccg tgcaattgaa attccagtac
     6121 atcatgccga gcgtgagttt ggtgaatcca aatacagttt tatgcgcctg attaatttga
     6181 tgtacgacct ggtgacctgc cttaccacaa cgccgctacg tatgctgagt ctgctcggca
     6241 gcattattgc gattggaggt tttagcattg cggtgctgct ggtgatttta cgcctgacct
     6301 tcggaccaca atgggcggca gaaggcgtct ttatgctatt tgccgtgctg tttactttta
     6361 ttggcgctca gtttatcggc atgggattac tcggtgaata tatcggcagg atctacaccg
     6421 atgtccgcgc ccgcccccgc tattttgttc agcaagttat ccgtccatcc agcaaggaaa
     6481 atgaataatg aaaaccgtcg tttttgccta ccacgatatg ggatgcctcg gtattgaagc
     6541 tctgctggct gccggttacg aaattagcgc catttttacc cataccgata atcccggtga
     6601 aaaagccttt tatggttcgg tggctcatct ggcggcggaa agaggcattc cggtttatgc
     6661 gccggataac gttaatcatc cgctgtgggt ggaacgcatt gcccaactgt caccagaggt
     6721 gattttctct ttttattatc gccatcttat ttgcgacgag attttgcagc tcgctcccgc
     6781 aggtgcattt aatctgcacg gttcgctgtt accaaaatat cgtggtcgcg cgccgctgaa
     6841 ctgggtgctg gtgaacggtg aaacggaaac gggcgttaca ttgcaccgaa tggtaaaacg
     6901 tgccgatgct ggggccattg tggcgcaact gcgcgttgcc attgcgccag acgatatcgc
     6961 tattacgctg catcataaat tgtgccatac cgcgcgccag ctactggaac agacattacc
     7021 cgccattaaa cacggtaata ttctggaaat cgcccagcgc gaaaacgaag ccacctgttt
     7081 tggtcgcaga acgccggatg acagtttcct tgaatggcat aaaccggcat ccgtactgca
     7141 caacatggta cgtgccgttg ccgatccgtg gccgggtgcc ttcagctatg ttggcaatca
     7201 gaaattcacc gtctggtcgt cgcgtgttca tcctcatgcc agcaaagcac agccggggag
     7261 cgtgatttct gttgcgccac tgctgattgc ctgtggcgat ggcgcgctgg aaatcgtcac
     7321 cggacaggcg ggcgacggca ttactatgca gggctcgcaa ttagcgcaga tgctgggcct
     7381 ggtgcaaggt tcacgcttga atagccagcc tgcctgcacc gcccgacgcc gtacccgggt
     7441 actcatcctc ggggtgaatg gctttattgg caaccatctg acagaacgcc tgctgcgcga
     7501 agatcattat gaagtttacg gtctggatat tggcagcgat gcgataagcc gttttctgaa
     7561 tcatccacat tttcactttg tcgaaggcga tatcagtatt cattccgaat ggattgagta
     7621 tcacgtcaaa aaatgtgatg tcgtcttgcc gctggtggcg atagccacgc cgattgaata
     7681 tacccgcaac ccgctgcgcg tatttgaact cgattttgaa gagaatctgc gcattatccg
     7741 ctactgcgtt aagtaccgta agcgaatcat cttcccgtca acttcagaag tttatgggat
     7801 gtgtagcgat aaatacttcg atgaggacca ttctaattta atcgtcggcc cggtgaataa
     7861 accacgctgg atttattcgg tgtcaaaaca attacttgat cgggtgatct gggcctatgg
     7921 cgaaaaagag ggtttacagt tcaccctctt ccgcccgttt aactggatgg gaccacgact
     7981 ggataacctt aatgcagcgc gaattggcag ctcccgcgct attacgcaac tcattctcaa
     8041 tctggtagaa ggttcaccaa ttaagctaat tgatggcgga aaacaaaaac gctgctttac
     8101 tgatatccgg gacggtatcg aggcgttgta ccgcattatc gaaaatgcgg gaaatcgctg
     8161 cgacggcgaa attatcaaca ttggcaatcc tgagaacgaa gcgagcattg aggaactggg
     8221 cgagatgctg ctggcgagct tcgaaaaaca tccgctgcgc catcatttcc caccgtttgc
     8281 gggctttcgc gttgtcgaaa gtagcagcta ctacggcaaa ggatatcagg acgtagagca
     8341 tcgtaaaccg agcatccgca atgcccaccg ctgcctggac tgggagccga aaattgatat
     8401 gcaggaaacc atcgacgaaa cgctggattt cttcctgcgc accgttgatc ttacggataa
     8461 accatcatga ccaaagtagg cttacgcatt gatgtcgata cctttcgtgg cacccgtgaa
     8521 ggcgtgccgc gtctgctgga aatcttgagt aagcataata ttcaggccag catttttttc
     8581 agcgtcggcc cggacaatat gggccgccat ctctggcgac tggtgaagcc acagtttttg
     8641 tggaagatgc tgcgctcaaa cgcggcatcg ctttatggct gggatatttt actggcaggt
     8701 acggcctggc caggtaaaga gattggtcat gccaatgccg atatcattcg tgaagcggct
     8761 aaacatcacg aagtcggcct gcacgcctgg gatcaccatg cctggcaagc ccgtagcggt
     8821 aactgggatc ggcaaacaat gatcgacgat attgcacgcg gtcttcgcac tctggaagag
     8881 attatcggtc aaccggtaac ctgttctgcc gctgcgggct ggcgtgccga ccagaaggtg
     8941 atcgaagcaa aagaagcgtt ccatttgcgc tacaacagcg attgtcgtgg ggccatgccg
     9001 ttccgtccac tgctcgaatc aggaaaccct ggcactgcgc aaattccggt gaccttaccc
     9061 acctgggatg aagtgattgg tcgggatgtg aaagcagaag attttaacgg ttggttactc
     9121 aaccgcatcc tgcgagataa aggcacgccg gtttatacca ttcatgcaga agttgaaggc
     9181 tgcgcttatc agcataattt tgtggatctc ctcaaacgcg cagctcagga aggcgtgaca
     9241 ttttgccctt taagcgaact gttatcagag acgttgccgc tcggacaagt tgttcgcgga
     9301 aatattgccg gacgtgaagg ctggctgggt tgccaacaaa ttgcgggtag tcgctgatga
     9361 aatcggtacg ttaccttatc ggcatcttcg cgtttattgc ctgctattac ctgttaccga
     9421 tcagcacgcg tctgctctgg caacccgatg aaacgcgtta tgcggaaatc agtcgagaaa
     9481 tgctggcatc cggcgactgg attgtgcccc atctgttagg gctacgttat ttcgaaaaac
     9541 ccattgccgg atactggatt aacagcattg ggcaatggct atttggcgcg aataactttg
     9601 gtgtgcgggc aggcgttatc tttgcgaccc tgttaactgc cgcgctggtg acctggttta
     9661 ctctgcgctt atggcgcgat aaacgtctgg ctctactcgc cacagtaatt tatctctcat
     9721 tgtttattgt ctatgccatc ggcacttatg ccgtgctcga tccgtttatt gcattctggc
     9781 tggtggcggg aatgtgcagc ttctggctgg caatgcaggc acagacgtgg aaaggcaaaa
     9841 gcgcaggatt tttactgctg ggaatcacct gcggcatggg ggtgatgacc aaaggttttc
     9901 tcgcccttgc cgtgccggta ttaagcgtgc tgccatgggt agcgacgcaa aaacgctgga
     9961 aagatctctt tatttacggc tggctggcgg ttatcagttg cgtactgacg gttctcccct
    10021 ggggactggc gatagcgcag cgggagcctg acttctggca ctattttttc tgggttgagc
    10081 atattcaacg ctttgcacta gatgatgccc aacatagagc tccgttctgg tactacgtgc
    10141 cggtcatcat tgccggtagc ctgccgtggc tgggattact ccccggtgca ctgtacacag
    10201 gctggaaaaa ccgcaagcat tccgcaaccg tctatttgtt gagctggacg ataatgccgc
    10261 tgctgttttt ctccgtcgct aaaggcaaat tgcccaccta tattctttct tgctttgcac
    10321 ctctggcaat gctgttggcg cattacgctt tgctggcagc aaaaaataat cctctggcgc
    10381 tgcggattaa tggctggatt aacatcgctt ttggcgtcac tggcattatt gccacatttg
    10441 tgatctcccc ctggggacca atgaacacgc cggtgtggca aaccttcgag agctataaag
    10501 tcttttgtgc ctggtcgatt ttttcgctat gggcattttt cggctggtac accttaacaa
    10561 acgtcgaaaa gacctggtct ttcgccgcgc tttgcccgct ggggctggcg ttgctggtag
    10621 gattttcaat tcctgacaga gtcatggaag gaaaacatcc gcaatttttt gtcgagatga
    10681 cacaagaatc acttcagcca agccgctata ttcttactga tagcgtcggt gttgccgcag
    10741 gtctggcatg gagcctgcaa cgcgatgaca tcatcatgta tcgccagaca ggtgagttga
    10801 aatacggcct taattatccg gatgcgaaag ggagatttgt cagcggtgat gagttcgcaa
    10861 actggcttaa tcaacatcgt caggagggga ttattacact cgtgctttcg gttgaccgcg
    10921 atgaagatat caacagtctc gccattccgc ccgcagatgc catcgatcgt caggagcgtc
    10981 tggtgctgat tcagtatcgt cccaaatgat ctggctaaca ttagtctttg ccagcttgct
    11041 tagcgttgcc gggcagttgt gtcagaaaca ggcaacctgc tttgtggcga taaacaaacg
    11101 gcgcaaacat atcgtgctgt ggctgggact ggcgctggct tgtcttggtc ttgccatggt
    11161 gctctggctg ctggtcttgc agaacgtacc ggtaggcatt gcctacccga tgttaagtct
    11221 gaattttgtc tgggtgacgc tggctgcagt aaaactgtgg cacgaaccgg tatcgccgcg
    11281 tcactggtgt ggggtggcgt tcattattgg tggcattgtg atcctcggga gtacggtgta
    11341 atgggcctga tatggggatt attcagcgtc attattgcct cagttgcgca attaagcctg
    11401 ggttttgcgg caagtcatct gccgccgatg acgcacttat gggattttat cgcggctctg
    11461 ttagcttttg gcctggatgc ccggatactg ttactcgggc tgctgggtta tctgctttcc
    11521 gttttttgtt ggtataaaac gctacataaa cttgcgctaa gcaaagccta tgccttgctc
    11581 agtatgagtt atgtgctggt atggattgct tcaatggtac ttcccggctg ggaagggact
    11641 ttttcgctga aagcactact gggagtagcc tgtattatga gcgggttgat gctgattttc
    11701 ctgcccacga caaaacaacg ttactgagtt ttccctgccg ctgtacactg ccgttcccac
    11761 tcatcggggc tataactgct tgccgagagg actttcaccg tatgcagctt ttcacgatta
    11821 atacaataaa gcgcattctg caaaggcgag agtaaatccc ccactttcac ggcgaagtca
    11881 ctcttcactt cggcgatcat ttttatcgcg ccgccagcat cgcagagcat aagtacatgt
    11941 ctgttatctt gtttattgca gcacgatttt ttgtgccagc cattccattg cattatcctg
    12001 tttgctaaga gttttcacat caatttacag attaacagat aaactcttta tcaacggcaa
    12061 tggaatggca gaaatgagaa gtgaaacgga gggttaatta ttgctgacgt tgcacccact
    12121 cttttagcgc ctgacgtgaa attttaatac cgccgttttt cagctccggc ggtaaagtca
    12181 gccagcgcac cggttgttga aaacgagcca gcttatcttt cacccattcg ccaagatcaa
    12241 cggtttgctg gtcatactcc atcaccgcta ccggccgatg accaaactcc ttgtcggcaa
    12301 cgggaacgat aaacacctgc aaaaccgcag gatgtgcagc aataacacgc tcgacttctt
    12361 ccggctgaat accctcaccg ccactgaaga acagattgtc taaacgtccg acaatggtca
    12421 gcttgccatt atgcatctca ccgcgatcgc gcgtagcgta ccagccttcg tcattaacca
    12481 gtgaaaccag ttgcccgtta cgccagtaac cttctgccat actggcagcc cgcagccaca
    12541 cttcattatt aacgattttc acttcccgac ccggcagcgg caaaccaacg tctgccaggc
    12601 cgtcggcttc tttcgcacat accgtggagg caaactcggt cagaccatag ccgcaaaagc
    12661 aacgaatccc ctgctcgcgc gcctgttccg tcaactcgac cgggatagcc gcgccgccaa
    12721 gtaacaccgc tttcagtgaa acggaactgc ggttaaccag caaacgccag agttgtgttg
    12781 gcaccagtga agcgtgagta cagcctgcca gcatttgctc caatggctgt ttatcacgca
    12841 ccgtcatccg cgcaccagcg tataaccagc gccacataat tccctgaccg gagacgtgaa
    12901 acagcggtaa agagagcaac cagtcatcgt gatcgccaaa cggaatcagc gataacacac
    12961 cttgcgcact ggcaagatgg gcctgataag tatggacagc ggctttcggc aaaccggtag
    13021 aacccgaggt caacgtcatt gagcacagac gcgtcggctg ccacgcagcg gcatgtacgc
    13081 cttcaaccag ctgaatgtgc agcgacgtta atgccggaaa cgtgttttcc ccatccggca
    13141 ccagcgcaaa ttgcagcgtc agattgggca gcaattcttc aagcaacggt tgcggcagct
    13201 gagggttcac gggcaacacc cgcgccccgc attgcagtaa cgccagccag gcgagcagcg
    13261 tttgcggcgt attccacgcc cgcaacatca cgccgctgcc ctcaaccaca ccctgcgccg
    13321 caaagcccgt agctaactca tcgacgcgag cacaaagctc gcgccagttg agttgctcgt
    13381 cattaagacg taaggcgatg gcttctcccc gcacttgccg ccagtgacgc cacggccagt
    13441 cagagaagat cataacaact gctccagtac atcagcgtcc actaacggca gtgggctccc
    13501 cggccagcga cgaacctgct gcgcctgcat cagatccagc gtgtccagtc ccggaatggt
    13561 gtctggcgtt aaccaggcag caatccgcgc cagttgcgtt agacctaagc tcgactcaat
    13621 ggaagaactg atcaccgccg tcagccccag cgcatgcgcc gcctgtacct gctcgcgtac
    13681 tttttccaga ctgcccgtga gcgtgggttt gataactacc gcgcgcacgc cctcttcagc
    13741 cacaaaggca aaatccggct cgcgcaggct ttcatcccag gcaatggcaa tgccggtttc
    13801 acgggcaaac gctcgcgaat catcgcgggt tttgcacggc tcttcgagaa acgcgatgcg
    13861 gtggcgataa tcagggttaa catatttggc aaactgctga cctttcagcg gtgtccaggc
    13921 gcgatttgcg tcaagacgca aatgcagatc cggaattgcc tccagcaaca gattcaccac
    13981 catgccgtcg cgcaccgctt cgtacaatcc cactttgacc ttcgccactt tctcgcccgg
    14041 catatcggca agtttgagga tcagatcgtc cggatcgcca ttacacagcg gtgccgcacg
    14101 gtagttggct gcttgcggca acgtatctgt cagttctgcc aatgcacagc ttacgccaaa
    14161 ggccacggaa ggcatctgcg gtagctcgca atcgcctgcc agccagttat ttacccaggc
    14221 aagcagcacg ctttgcgcct cttcccaggt ttcctggctg aagcccggca gtggggagat
    14281 ctccccccac ccttcgcgct cgccctcacg caggcaaacg tacagcccgt cgcgggtttt
    14341 taaccgcctg tcgcgcagaa ccacccccgc gtccatgggg atctgccagc ggtatacctg
    14401 cgcgctacgc attacggatt ccgtttaaat ttgctgaaat caggctgacg tttctggttg
    14461 aaagcgttgc gaccttcctg accttcttcc gtcatgtaga acagcatggt ggcgttgccc
    14521 gccagctcct gcagccccgc ctgcccgtca cagtcggcgt tcagcgcagc tttcaggcag
    14581 cgcagcgcca tcgggctgtt ttgcagcatt tcgcggcacc aacggacggt ttctttttcc
    14641 agatccgcca gcggtaccac ggtgttgaca aggcccatat ccagcgcctg ttttgcgtcg
    14701 tactgacggc acaggaacca gatttctcgc gcttttttct gcccgacgat acgagccatg
    14761 taggaagcgc cccagccgcc gtcgaaggaa ccgactttcg ggccagtctg accgaagatg
    14821 gcattatctg ctgcgatagt caggtcgcac atcatgtgca gaacgtgacc accgccgatg
    14881 gagtagccag ccaccatcgc gacaaccggt ttcggacagg tacggatctg acgctggaag
    14941 tccagcacgt tcaggtgatg tacgccggaa tcatctttat agccgccgta atcaccacgc
    15001 actttctggt caccaccgga gcagaacgct ttatcgcctg cgccagtcag aatgatcacg
    15061 ccgatattgt cgtcataacg cgcatctgcc agtgcctgga tcatctcttt taccgtcaga
    15121 ggacggaagg cattgcgcac ctgcggacga ttaatggtga tttttgcgat accgtcggtg
    15181 gatttttcat aacgaatgtc ctcgaaacct tcggagcagt cgtgccattc aaccggtgcg
    15241 taaggcattg cttcatcagg ataaatcata ttgtgtcctt tagtcagaaa cgcaagatct
    15301 gcgccagact tgcgattacg ccagcgggat tttcccgatg cgcgttatgt ccggcgcgag
    15361 gaatgacatg gcagtcggca gccagttccg ccgccagggc gcggaatttg ctgtcacgtt
    15421 caccacataa ataataaaac gcaaatgtgc gggcgctaag gttagcacgt aaatcaggct
    15481 ggacggcgag agaagtcgcc tccagcatgg cggcaagggt tgcgccatta ttgttgctgc
    15541 gcagcgccac cagctcccgg cgttgatcgt cattgagcga ggcaaaaaca ggctgttgat
    15601 accagtcggc aaataccgcc gttaacggtt ctgtgcgaaa acgctgcgcc cattggcgat
    15661 cggaacgctg acgttccgca cgttgttcag cattttgcag ccccggatgc ccgccttcga
    15721 caataacccc acaaagcccc gccagcccct ggcaagccgc catcatcgcc acccgtccgc
    15781 caagcgagta ccccaccagc cagaagtcaa ggatattgta actaaccaag gttttacgca
    15841 gtaagtcggt gacatcatca aatccatcga cgctaatcgc cgccgaacca ccatgacctg
    15901 ggagatcaac atacaaccgt gagtagtcgg caaacgcctc gcccacttct tgccattcgt
    15961 ggcaatcgcc ggaaaaaccg tgcaaaaaca ccagccaggg taaacctggt tttccgtgtt
    16021 ttgcctgcgc gtgcaggatc ataaatggct tacctgcgcc agaagttgct ggagcgtttg
    16081 cgcaccatcg gtgtcgttaa ccaccatttc aatcaccgtg gtggttggcg tgcgccaggc
    16141 gtcggcaaat gccgtttcaa gttcctgcca gttttgcgga cgatgatatt tcagctcgaa
    16201 catcgcggcg gcgtgctcaa aatggacgtt ttgcggcatc agatagaaac gctcacgctc
    16261 gctttgcggc gttggcaaca gcgagaaaat ttgcccgccg ttgttgttca ccacaattaa
    16321 taccagcggc gcagaaacct gacgcaataa cgccagcgcg ttgagatcgt aaagtgcgga
    16381 gagatcgccc acaatcgcca gcgtcggttt gccgcttgcc cgctgaacgc ctgcggcggt
    16441 cgaaagcagt ccgtcgatac cgctggcccc tcggttgctg tacaccgggt aacctgccgg
    16501 aagttgcgaa agcgcatcaa tcagacgtac caccaggctg ttaccaacaa acaattgccc
    16561 ctgttcaggc agatagtcgc tgatacgatg cgccagttgc gcttcgccaa acgcatcacg
    16621 gcgggcaata accgcctgca ttgcctgttc cgccaggcgc gggatttcaa cgcaccaggg
    16681 ctggcgtttt tctgccggat gcagctccag ccagtcggca atattggcaa ttaagcgacg
    16741 tccgcgatgg tgtgccggat caagtcgccc ttcaatgtca tcaacaatcc agtactcttc
    16801 tggttcacag cttgcctgcc attgcaggag ccgtttcccc gtcaggcagc ttcccagttg
    16861 caccacaatt tgcgcctgct gcagctcgct ggtcgctttg gcattgccta accaaagatc
    16921 ggcacacggc agcggctgcc cggtttgtga cagcacatcg ccaatcagcg gccagccaag
    16981 agtttgcgcc cacagggcaa cttttttgcc ctcttccgca ctcatgcgcc cggcaaccac
    17041 cacgccgcgc ttttgtcgcc agaagaacca gtcgcgctgt ttttcacttt ccagacgagg
    17101 cgcttcacgc agccacggtt tgtcgtcctg ccaccagtca cccagacgct gttgccagct
    17161 aagcccggta tcgtccattt cgccatacag cggttcagca aacgggcagt tgatatggac
    17221 tccccccaca tgaagcgtac cgagagcgtg gtcgatggta gaaaccagcc aacgtgcggg
    17281 gatatcctgg gtcgggcgcg gcaatgaaat actgtgcgtg gggtgagagc cgaacattcc
    17341 cggctggcga attgcctgat tcgcgccgca gtcaattagc tccggcgggc gatcggcggt
    17401 taagagaatc agtttttctc cggttaaccc ggcttcaatc agtgccggat agagatttgc
    17461 caccgccgtg ccggaggtca caatcaccgc caccggctgc ttgctgactt tcgccagccc
    17521 cagcgccaga tgccccaacc cacgctcatc gaaatgggtg tggtgaatga atgcggaatt
    17581 ctccgccgcc gctaacgtta acggtgtaga acgcgagcct ggggcgatac agatgtgtct
    17641 gacgccgtga cgcgttaatg cttccagaat gaccgccgcc cagcgtcggt taaatgcgct
    17701 tactgacatg agtttgtccg gtatcaatat tgcggctaag tataaggagc tacaaaaata
    17761 gattattgat atgaatcggt aatgatgcga ctcattacta ttccatttgt aataaagtac
    17821 gcagcactgc cgctttgttg tcgatttcct gccactcttg ctcggggtcg gaaccacgga
    17881 caatgcccgc gccagcatat aatcgcacga cattgccgct aatttttgct gagcgcaggg
    17941 aaacgcagaa ttcgctttgt tgtaatgaga gatagcccgc agaaccggcg taccattctc
    18001 gggtgaacgg ttcgtgacgg gcgataaact gtcgcgccag atcgcgcggt aagccagcaa
    18061 ctgctgccgt tggctgcaac tgatgtaaac agatcacatc atccgctttg ttgagtgaag
    18121 tccagataca gcggcgaaga tgctgcactt tacgcagacg cagtacctgc ggcggtaaaa
    18181 catccagcgt ctgggtatcg gcctgtaatc gttggcagat atcttccacc accagcatgt
    18241 tctcgcgctg gtttttatca tccgccatca gccactctcc taactgctgc gcctgcttat
    18301 catcaggatg atttgctact gttcccgcca gcgcttcagt acgcagcgct ttgtcacgcc
    18361 gccgccataa ccgttccggt gaagagccaa gaaaagcatt ttcgccatca aaggccatgt
    18421 aaaaatggta gcaattcaga ttcagtcgac gactggcagc catcatcgcc gccgcgttga
    18481 ccggacttgc gaaatgcagg tcagttgccc gagcgagcac cactttgtcg agctcacctt
    18541 cggcgatggt tttcgttgcc agttcgatta attgcgtcca gcccgttttg tccggccagt
    18601 gttgttctcg cgtggtggtt aaatgtaacc caggcaaggg cttgatactc accagtgtgg
    18661 cgataaattc ttttgcctga atcgcatcgt gctgaaggga gctttcgctg aatagcgtca
    18721 gccgcagcgt ggctttaccg ccacagcgtc gccattccag gcggggtaaa agtaaattgc
    18781 cctgcgacgg gtcaaaagca ttcagccccc aaatgcgtaa gtcggcgtgt tccgggtgct
    18841 ggcgaagaaa acgttgtgcc tggtccaacg acgtaaaacg ggtaatcgcg cccagggcgg
    18901 cagcttcttc atcaccatta cgttgttgcc agtagaattg cgggtacgtt tgctgactgg
    18961 ccagccagct caaggcatca aaagcgtctt tgagagggaa aggaatatcg ataacccgaa
    19021 tgccgggtgt cgccggaatc tcttgcgaca aatggcgcag tagattttcc agcgccgtag
    19081 taagtgattg cacgcggacc tctccctgtt aaaagcctca cattatacgg ggtactacaa
    19141 aaaaaatgca gtaccccggt gtagggaggt tttaacggcg tgccagcaac agtcctagta
    19201 ccagcccgac ggccgcgccc acaccaattc cttgccaggg tttttcgtgg acgtagtcat
    19261 cagcacgata aacagcctgc ttcgcccgat agtaataact gtctgaagcc tggctaaccc
    19321 gttttttcac atcatccagc gctttttctg cacgcgcttt cagctcaaca tatttctgat
    19381 cggcgggatc gccagaggag cggagcacct cttccagtgt ttcactaagc agcgtcaggt
    19441 cgtcatcgat acgtgtatca ccaaactgat tagacattct cgttctccat ttgcgtaaaa
    19501 cctgtcacta actatagaca agagttgttg attacgcctg aattacctcg cgtgccatcc
    19561 caatgtgtgg gataccatct tcttcataga cctccgtcac agggataaaa ccgaaactct
    19621 ggtagaagtt ttgcagatgc gcctgcgccc cgaggtaaac aggcttatca ggccagtgat
    19681 gcgtacatgt ttcgagtgtt ttactcatca gctgctgccc cactttttca ccacgcaacg
    19741 cttcactgac aatcacccga cctataacga ccggctcaag atcatcatca cttttcagaa
    19801 tcctcgcata cgccaccagt tcatcatttt tccaaccgag aatatggcga ttatcccccg
    19861 tcaaatcatc accatcgata tcctgataag ggcaattctg ctcgacaaca aacaccgcgc
    19921 aacgcagttg taataaggca taaagctggg aaaccgataa ttcagagtga tgcagatctt
    19981 gccattcaat cataatgctc tccttttaga tgtatgcctt atactagccg gaatattttt
    20041 tgaaacgtga tgaactcatg gaattaattt ttttaggtac ttcagccggt gtgccaaccc
    20101 gcacgcgcaa tgtcacggca atattgctta acctgcaaca tccgacccag agcggacttt
    20161 ggttgtttga ctgcggtgaa ggcacccagc atcagctact gcataccgcc tttaaccctg
    20221 gaaaactgga caagattttt atcagtcacc ttcatggcga tcatcttttt ggtttacccg
    20281 gcttgctgtg cagtcgttct atgtcaggca ttatccaacc cttaacgatt tatggtccgc
    20341 aaggtatccg tgaatttgtg gaaaccgcgc tgcggattag cggctcatgg accgattatc
    20401 cgctggaaat tgtcgaaatt ggcgctggcg aaattctcga tgatggcctg cgcaaagtaa
    20461 ccgcttatcc gctggaacac ccactggaat gttatggcta tcgtattgaa gaacatgata
    20521 aaccgggtgc attaaatgcc caggcattaa aagctgctgg cgtgccgcct ggcccactgt
    20581 ttcaggaatt aaaagcgggc aaaacaatca cgctggaaga tggaaggcag attaacggcg
    20641 cagattactt agctgctcca gtgccaggta aagcgctcgc tattttcggc gataccggcc
    20701 cctgcgatgc cgcacttgac ctggctaaag gtgtcgatgt catggtgcac gaagcgacgc
    20761 tggatataac catggaagcc aaagccaata gtcgcggcca tagctctaca cgccaggctg
    20821 cgacactagc ccgtgaggct ggagtcggca agctaatcat tacccacgtc agctcgcgct
    20881 atgatgacaa aggttgtcaa cacctgttac gtgaatgcag gtcaattttc ccggcgactg
    20941 aactggcgaa tgatttcacc gtgtttaacg tttaactctg cttattatta aaaacagggt
    21001 gaaacttgcc ctgttatagc aacccgcgct acatatatcc acccacacag gcatattgag
    21061 cgatttttga tatttattca tctccttacg cactatattc agagcaataa gttgcctctt
    21121 ttcatcttga ttcagcacaa cctttgcaac taaaaggagc ccatgatggt tacagttgtc
    21181 agcaattatt gtcaattatc tcaaacccaa ctcagtcaga catttgcaga aaaatttact
    21241 gtgaccgagg aattactgca gtctttaaaa aaaacagcgt tatccggaga tgaagaaagt
    21301 attgagttac tgcataatat tgcgttaggt tatgatgaat ttggaaaaaa agctgaagat
    21361 attctttacc atattgttag aaacccaaca aatgagaccc tatcgattat ccgacttatt
    21421 aaaaattcct gtttaaaatt atataatttg gcacataccg caaccaactc ccaccttaaa
    21481 ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc cccttcgaaa
    21541 ttaatgacaa ttatcggtaa tgaaattcct cttatatctg aaaaacagtc gctttcaaag
    21601 gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg ggataaaaat
    21661 cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc taatgcaaaa
    21721 aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag aactcacctg
    21781 gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga agtccagtta
    21841 ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat agtaaataaa
    21901 aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca ctaggccatg
    21961 ataaagaaaa ttcacttaag cgtgtcatta atagttttaa ttctgaactc atgggagaaa
    22021 tgtcgaataa taatataaaa gttcatttaa atgaatcaga gataatattt ttacatgccg
    22081 atcttcagca atacttaagc caaagttgcg gtgcgtttgt gtgcatggca gcccaggaag
    22141 tgattgaaca aagggaaagc aattctgaca gcgcccccta tacattatta aaaaactatg
    22201 ctgacagatt taaaaaatat tcagcagaag agcagtacga aattgatttt caacatcgac
    22261 tggtaaacag aaattgttat ttagataaat atggcgatgc acgaatcaat gcttcttata
    22321 cacaattaga aataaaacac tcacaaccca aaaatagagc atccggcaaa agagtgagtt
    22381 aacatcattg ttaatcaatt cgaccagtca gtaaatatgc tgactggtcg aacataattt
    22441 gctaaaaagt catcactgac tgatgtcagt cccaccatct gccagttcaa tctacgggta
    22501 tttccagcgt atcgcggcac gcgattgctg gtctgttgat gtagtggatt gtagttgctg
    22561 acttttctta ccctcagatt tctgccccac ttgtcaattc aatctgccaa taaataacat
    22621 aattatattt ttattcctca cagaaattcc tttttaaaaa tagcgaaccc actgtaaaaa
    22681 acactttttg tatacctcta ccaagcgtaa taaaataaac gctcataatt cattataaat
    22741 aataatgaaa taacgccttt tattgataat ttacatatga aaaaaatttt ccttttgtta
    22801 tattatttct gttttcactc cctctgatta tttttaccaa ccctgggtca ctgcattatc
    22861 gccaacgccc cgacacgtaa gtcctgagca attagaaaaa aca
//
LOCUS       DADLSA010000052        22176 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.052, whole genome shotgun sequence.
ACCESSION   DADLSA010000052 DADLSA010000000
VERSION     DADLSA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.052"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            139..1320
                     /gene="nimT"
                     /locus_tag="JN691_002051"
     CDS             139..1320
                     /gene="nimT"
                     /locus_tag="JN691_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HAY7243502.1"
                     /translation="MTCSTSLSGKNRIVLIAGILIIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            1375..1722
                     /locus_tag="JN691_002052"
     CDS             1375..1722
                     /locus_tag="JN691_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAY7243503.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKNQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(1744..1998)
                     /gene="yoaF"
                     /locus_tag="JN691_002053"
     CDS             complement(1744..1998)
                     /gene="yoaF"
                     /locus_tag="JN691_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAY7243504.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            2181..2738
                     /gene="dgcP"
                     /locus_tag="JN691_002054"
                     /pseudo
     CDS             2181..2738
                     /gene="dgcP"
                     /locus_tag="JN691_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
     gene            2782..3747
                     /gene="yeaX"
                     /locus_tag="JN691_002055"
     CDS             2782..3747
                     /gene="yeaX"
                     /locus_tag="JN691_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HAY7243505.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(3801..4916)
                     /gene="rnd"
                     /locus_tag="JN691_002056"
     CDS             complement(3801..4916)
                     /gene="rnd"
                     /locus_tag="JN691_002056"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAY7243506.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(4998..6683)
                     /gene="fadD"
                     /locus_tag="JN691_002057"
     CDS             complement(4998..6683)
                     /gene="fadD"
                     /locus_tag="JN691_002057"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAY7243507.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEVKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(6888..7469)
                     /gene="yeaY"
                     /locus_tag="JN691_002058"
     CDS             complement(6888..7469)
                     /gene="yeaY"
                     /locus_tag="JN691_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAY7243508.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTLQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(7509..8204)
                     /gene="tsaB"
                     /locus_tag="JN691_002059"
     CDS             complement(7509..8204)
                     /gene="tsaB"
                     /locus_tag="JN691_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAY7243509.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(8262..10172)
                     /locus_tag="JN691_002060"
     CDS             complement(8262..10172)
                     /locus_tag="JN691_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAY7243510.1"
                     /translation="MTDDFAPDGQLAKAIPGFKSREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQRALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            10304..10648
                     /locus_tag="JN691_002061"
     CDS             10304..10648
                     /locus_tag="JN691_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY7243511.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            11010..11369
                     /locus_tag="JN691_002062"
     CDS             11010..11369
                     /locus_tag="JN691_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAY7243512.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11489..11668)
                     /locus_tag="JN691_002063"
     CDS             complement(11489..11668)
                     /locus_tag="JN691_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAY7243513.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            11742..13103
                     /gene="pabB"
                     /locus_tag="JN691_002064"
     CDS             11742..13103
                     /gene="pabB"
                     /locus_tag="JN691_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAY7243514.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            13107..13685
                     /locus_tag="JN691_002065"
     CDS             13107..13685
                     /locus_tag="JN691_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAY7243515.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            13869..15233
                     /gene="sdaA"
                     /locus_tag="JN691_002066"
     CDS             13869..15233
                     /gene="sdaA"
                     /locus_tag="JN691_002066"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY7243516.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            15364..16962
                     /locus_tag="JN691_002067"
     CDS             15364..16962
                     /locus_tag="JN691_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7243517.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(16966..18516)
                     /gene="yoaE"
                     /locus_tag="JN691_002068"
     CDS             complement(16966..18516)
                     /gene="yoaE"
                     /locus_tag="JN691_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAY7243518.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            18979..19950
                     /gene="manX"
                     /locus_tag="JN691_002069"
     CDS             18979..19950
                     /gene="manX"
                     /locus_tag="JN691_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAY7243519.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            20013..20813
                     /locus_tag="JN691_002070"
     CDS             20013..20813
                     /locus_tag="JN691_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY7243520.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            20826..21677
                     /locus_tag="JN691_002071"
     CDS             20826..21677
                     /locus_tag="JN691_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY7243521.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            21732..>22176
                     /locus_tag="JN691_002072"
     CDS             21732..>22176
                     /locus_tag="JN691_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAY7243522.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
BASE COUNT         5352 a         5757 c         5572 g         5495 t
ORIGIN      
        1 tcggtcgggt tcatagccat tgagattcaa cctgtgcatc attttgtccg aacttagcga
       61 taatttgtca ttttagcttg attcaacata acaataaaaa cggtaaggta cagcctcgtt
      121 tgtaacaatg agaagcatat gacctgttca acttcattaa gcggcaaaaa caggattgtc
      181 cttatcgctg gcattctgat aattgccaca acattacgcg tcacctttac cggcgcagcg
      241 ccgttactgg atacgattcg ttccgcttac tcgctgacga cagcgcaaac cggcttatta
      301 accaccctgc cattattggc ctttgcgcta atctcacctc tggctgcccc ggtggcgcga
      361 cgttttggta tggaacgtag cctgtttgcc gcgttacttt tgatctgtgc aggtatcgca
      421 attcgttctc tcccttcgcc ttacttatta tttggcggta cagcggtcat tggcggtggg
      481 attgcattag gcaatgtctt actgccagga ttaattaaac gcgatttccc tcattccgtc
      541 gccagactta ccggcgcata ttccctgaca atgggagctg cagcggcact gggatcggct
      601 atggtcgtgc cgctggcttt gaacggtttt ggctggcaag gcgcgttgtt catgctgatg
      661 tgttttcctc tgctggctct ttttttatgg ctgccacagt ggcgaagtca gcaacatgca
      721 aatttgagta cctcacgcgc cttacatact cggggtatct ggcgttcgcc acttgcctgg
      781 caggtaacat tgtttcttgg gatcaactca ctggtctatt acgtgattat tggctggctt
      841 ccggcgatcc tcatcagtca cggctatagc gaagcacagg cgggttcact gcatggtttg
      901 ctgcaactag ccacagcagc tcccggtttg ctgatcccac ttttcttaca tcatgtgaaa
      961 gaccagcgtg gtattgcagc gttcgttgcc ttgatgtgcg cagtgggcgc ggttgggctc
     1021 tgctttatgc cagcgcacgc gatcacctgg actctgcttt tcggttttgg ttccggcgca
     1081 acaatgatac tggggttgac gttcattggt cttcgggcaa gttctgcgca tcaggcggcg
     1141 gcactctcgg ggatggcaca atccgtcggg tatttgctgg cagcctgtgg gccgccgcta
     1201 atgggtaaaa tacacgatgc taacggtaac tggtccgtac cactgatggg tgttgcaata
     1261 ctttcactac tgatggcgat tttcggactt tgcgccggga gagacaaaga aattcgctaa
     1321 tatccggtgc tatagtgacg tgacaaatct tgcgtgaaag ggagaacaaa cacgatgaat
     1381 attcagtgca aacgcgttta tgatccggct gaacagagcg atggttatcg cgtactggtc
     1441 gaccgcctct ggccgcgcgg tatcaaaaaa accgatttag cccttgatga gtgggataaa
     1501 gaaatcacgc cgtcaacgga actgcgcaaa gcctttcacg gcgaagtcgt cgattttgca
     1561 acctttcgcg agcaatatcg cgcagaactg gcgcaacacg agcaagaagg aaagcggctg
     1621 gcggacatcg ccaaaaacca gccgctgacc ctgctctacg cagcaaaaaa caccacgcag
     1681 aaccatgcgc tggtgctggc cgactggcta cgtagcttgt gattttagta cggcatccgg
     1741 cggttatttt tcaccagccg gatggtcccg tcgccacaat gcccattcat caatcgtttc
     1801 accgcccggt aatttgcaat tgttgctgac cccttgcgct gtctgcactg gaatgagcgt
     1861 cccgcccttc tgctggcaat agaccgacgc cggatttgcc ataccaatct gcggcggttt
     1921 aggtgcttct ggctgagaag gggttgaaca accagccagg accagcaagc aaggcagaac
     1981 aaaactgata attttcattt attgatctca catatttatc caagcttaga gtatcgcggt
     2041 atcgttttgt tttgcagcac tatttttatt acattcactc aaaacatatt acgtcttgtt
     2101 tcatctttgt tgatgatgtt ttatcatgcc tgcaaagatt aaataatcag cacttacccg
     2161 ccgtatcctg gagttgttcc gtgtcagatc agattatcgc ccgcgtctcg caatcccttg
     2221 ccaaagaaca gtcactggaa agtctggtcc gacagcttct ggagatgctg gaaatggtca
     2281 ctgatatgga atcaacctac ctgaccaaag tggatgtcga agcgcgcctg cagcatatta
     2341 tgtttgcccg taacagccag aaaatgcaca tcccggagaa ttttaccgtc tcgtgggatt
     2401 actcgttatg caaacgcgcc attgatgaaa actgcttttt cagcgatgaa gtccctgacc
     2461 gttggggcga ctgtattgcg gcacgcaatc ttggcatcac cacatttctg agcacgccaa
     2521 ttcacttacc ggatggatca ttctatggca cgctttgcgc cgccagcagt gagaagcgcc
     2581 agtggagtga acgcgcggaa caggttttac agttattcgc cggactgatt gcacaatata
     2641 ttcaaaaaga ggcactggtt gaacagctgc acgaagccaa tgctgcgctg attgcgcaat
     2701 cgtataccga ctcgttaacc gggctaccga atcggcggcg gtattttccg ccgctgggct
     2761 gatttttgat ggagtacagc aatgtcagac tatcaaatgt ttgaagtaca ggtgagccag
     2821 gttgaacccc ttaccgaaca ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg
     2881 ctgcctgcgt tcaccggagg aagtcacatc ctagtgcaga tgagcgatgg tgataaccag
     2941 tacagcaatg cgtattcact actgagttca ccgcataaca cctctagtta tcagattgcc
     3001 gttcgactgg aagaaaactc gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg
     3061 ggcgatcggt taacgatctc aacgcctaat aacctgtttg cgctaattcc ctcagccaga
     3121 aagcatctgt ttatcgcggg cggtattggt atcacccctt tcctgtcgca catggcagag
     3181 ttgcaacaca gcgacgtcga ctggcagcta cattactgct cgcgaaatcc agaaagttgc
     3241 gcatttcgtg atgagctagt ccagcatccg caggctgaga aagtccattt gcatcattca
     3301 tcaaccggaa cacgactgga attagcgcga ttattggcgg atatcgaacc tggcacacac
     3361 gtttatacct gtggccccga ggcgctaatt gaagcggtaa gaagtgaagc tgcgcgtctg
     3421 gacatcgccg ccgatacgct gcactttgag caatttgcta tcgaagacaa aaccggcgat
     3481 gcatttaccc tggtgcttgc ccgttccgga aaagagtttg tggtgccgga agagatgact
     3541 attttgcagg ttattgaaaa taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg
     3601 gtatgcggaa cctgcgaaac ggcaatactg gaaggtgaag ctgaccatcg ggatcagtat
     3661 tttagcgatg aagagcgtgc cagccagcaa agtatgttga tctgttgttc gcgtgcgaag
     3721 ggtaaacgcc tggtgttgga tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc
     3781 cagtccggct tcggaagatt ttactgcgga tattcctgca ataaattgtg taatgcttcc
     3841 gccatcagct caccacgcca gccggaaatc agctccggca aattgttctg cggtttcagt
     3901 ttccagtgcc agttcagcag ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg
     3961 atcttatgcg tttcgctcac gtcagtaatc agcgacttaa tcgctttaaa cgctttacga
     4021 taaccaggca tgtccatcag gttaagcatc ggctgcggta aggcatcttc cggcaatgtc
     4081 tgcgcttttt ccaccagcgc cagcagcgtt ttaccgtgaa agcggatttc gctaccggat
     4141 aaacccaggc tgtccagttc gcctaaactt cccggcatat aacgcgcgac cgaccacaaa
     4201 tgctcttcac gcacgacaaa gttcaccgcc agatcacgct ctcgcgcctt gcgcagtcgc
     4261 cagtcggcta acagttgcag acaggccagt tggcgtgtgc gtaactgcca ggcattggtg
     4321 atatcacgcc aggcatcttc cggcgcaacg acttcctgac gacgcatttg catcaggcgg
     4381 cattcatcca gcgccgcagg tagccagccg gaggcctccg tttctaccat aagcttggcg
     4441 gtgatcggta acagatacca gacatccgcc gctgcgtatt cacactgacg ttcggtcagc
     4501 ggtctggcca gccagtcggt gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct
     4561 tccaccatgg aagcgaaacc ccatgacatc gggcgtccgc agaaggcagc aaggatttgc
     4621 gtgtcaatca agggttgtgg taattcgcca aagacattga ggaacacttc cagatcttca
     4681 ctgcctgcat ggagaaattt tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac
     4741 cagtcggtga tcccgagagg atcaattagc gccagatgct cgccatcgaa aagttgaatc
     4801 aaccccagct gcgggtaata agtgcgcgta cgaacaaatt cagtatccag ggctatcgcc
     4861 ggaaaggcac ggacggcttc acacaaagaa gccagcgcat cgtccgtggt aatcatttgg
     4921 taattcaaat tgttttctct ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct
     4981 gacgactgac ttaacgctca ggctttattg tccactttgc cgcgcgcttc gtcacgtaat
     5041 tctcgtcgca aaatttttcc gacgttagat ttcggtaact catcacgaaa ctccaccagc
     5101 ttcggtactt tgtatcccgt gagctgacgg cggcaaaaag tcaccagtga ctcttcggta
     5161 agcgatggat cttttttcac tacgaagatt ttcaccgctt caccactgga gccggaaggt
     5221 acgccaacag ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg
     5281 ttgggataga cgttaaaacc ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc
     5341 aggaatcctt cttcatccat caccgcgatg tcgccggtgt gtaaccagcc atttttgatg
     5401 atctcatctg tagcatccgg acgctgccag taacccagca tcacctgcgg tcctttgaca
     5461 caaagctcac ccggttgacc cggtggtact tcgttgtcgt cgtcatccac cagtttgact
     5521 tccgtcgatg gcaccggcaa accgatgcta ccgctatgat aatcaatatc atacgggtta
     5581 acgctcacca gcggcgcgca ctcggtcagg ccataacctt ccagcagata ctgtccggtc
     5641 agtttcaccc agcgttctgc caccacttgc tgaacaggca tcccaccgcc tgcggaaaga
     5701 tgcagactgg agaaatccag ctgctggaac tctttattgt tcagcaacgc attgaacaag
     5761 gtgttaacgc cagtgatagc ggtaaacgga tatttcgcta actcttttac caaccctgga
     5821 atatcacgcg ggttagtgat aagcaggttc tgcccaccca gttcgataaa cagcaggcag
     5881 ttaatggtca gggcaaaaat gtgatacagc ggcagtgccg ttactaccag ttctttacct
     5941 ggatgcagca acggcccata ggtggcgttg acctgttcca ggttcgccag catattgcgg
     6001 tgagtcagca tcgcgccttt cgccacacct gtggtgccgc cggtgtattg caggaaggct
     6061 aaatcttccg gcaccagttc gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg
     6121 ctacgaaatg aaatggcatc tggcagatgg tatttcggca ccaaacgctt gatgtattta
     6181 acaacgaaat tgactaccgt gccttttgcc gtagatagct gatcgcccat acgggtcaga
     6241 attacgtgct gaacggcggt tttatcaacc actttttcca gagtgtgggc aaagttagac
     6301 acgataacaa tcgccgatgc gccgctatcg ttaagctgat gctcaagctc acgcggggta
     6361 tacaacgggt taacgtttac gacgatcatc ccggcacgca aaatgccaaa tagcgccacc
     6421 ggatattgca ataagttagg catcatcaac gcaacgcgat cgcctttctt cagccccaac
     6481 ccttgttgca aataagcggc aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatc
     6541 acctccccca tattcacaaa cgcaggctga tcggcgtagc gcgcgaccga ctgctcaaac
     6601 atatctacca gagattgata acggtcaggg ttgatctccg tcggaacgtc cgcgggataa
     6661 cggttaagcc aaaccttctt caattcttca cctctaaaat gcgtgttcgt cgtcatcgca
     6721 accccaaatg atatacatgc tgttaacata atattaactc atcataccag cttgataatt
     6781 acccaacgaa aaggttgcga agcgcgtcac tatttatttt tatctttacc gtaagaatgc
     6841 agaaacagcg gaccagccgc tgtttctttt tctttaaaaa caagcaatta ttcggttaca
     6901 actgtttgta ctctcgcggg gccgggatta taccagcccc atccgccgta gccatagggc
     6961 cagccacgac cgccataaaa ccatggatca atcggctgag gcggcataat cacctgcagg
     7021 gttaaatgcc aacgtttgta acccgttact tgcatcacca taaatttata gggcgtattg
     7081 ccgattttgc cgtcaaccgc accggtgatt ggcccgacta ccgtaaccag ttgtccacgg
     7141 aaatccaccg ggtccagaaa accgttcaca tcggcataaa tgcgaccgcg agaaggttct
     7201 cccagcgtcg gcctggctcc gctgtccagc ggtacggtag caatttccag gcgggttttc
     7261 ccttgctggt tttgtaccgc aaccactttg ccaccaaagc gtgcctcctg accaacgtac
     7321 agctgcggcg cactcatcac ccgaactaaa tcttgttgcg gcgtgggact gctgccttta
     7381 atggcgtccg gcacagtgac acaaccgctc agcattagcg caaacgtacc tgccagtatg
     7441 cctttgataa cattcttttg aaccgccatg atgcgactcc tttttttctc agggcatact
     7501 cttaagattc attctttgcc cggaagtttc ttccatgcga cgttgttacg caaataaacc
     7561 ggctcggcat gttccaccgc caccgtttta ccctcggcaa acatctgaca agcaatcggc
     7621 agcatatctt ctgcagcggg gagtaacact tcgccatcgc gcaaaaccag cccgctctct
     7681 ttaccgagat ccggccaggc ttgccagccc gtccctaccg tcacccattc gccggaaagc
     7741 tgttgcattc gttcatgaac gagttcgggt ttgagtacgg cttcggtttc ttcaccgtgc
     7801 cagataccgt tttcatcacg ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca
     7861 atggctgaca gcacgcgtgt tgcgccgttt ttgcgccacg ccccttgcgc catcgtcatt
     7921 agcgtggaga cgccaatcat cggtaattcc gcgccaagcg ccagcccttg cgcgatgcca
     7981 ataccaatgc gcacgccggt aaagctaccg gggccgcgcc cgtaagccag agcgttaata
     8041 tcagtcaggg aagttccgct ggtggtcagg atatcctgca ccatcggtaa gattcgttga
     8101 gtatgttcac gagggcaaag ctcaaaatga gcgttgacag taccgtcgtt ccacagggcg
     8161 acagagcagg cctctgtcgc ggtatcgata gccagaattc gcatgggtct tcgtgcttag
     8221 atcaataaaa aggcgcgcat cataccatac tccgtaacaa attacctgga ggatggtatc
     8281 gcaaggaaac gaaccgcacg ggcaatgtca cgggtgcgtg gcgcgggcgg cagactggcg
     8341 agaaacgtcg cgccgtaagg acgcatcacc agccgatagt cacaaatcac cagcacgcca
     8401 cgatcgtcgg cgtcgcgaat cagtcgacct accccctgtt tgagagtaat gacggcatct
     8461 ggtagttgca cttcatcgaa cgggtcgcca ccgcgcaaac gacaatcttc catgcgcgct
     8521 tttaacagtg gatcatccgg cgaggtaaac ggcaatttgt cgataattac caatgacaat
     8581 gtatcgccac gcacgtccac cccttcccag aaactactgg ttgccacaag aagcgcatta
     8641 ccggcactga caaactgctg caacagttgg cctttactgg tttccccctg caacaaaacg
     8701 ggaagcgtca tggtagcgcg gaactgctcg gccagatcgc gcatcatggc gtgcgaggta
     8761 caaagcataa aacaacgacc gttgttagct tcgatgatcg gtcgcagcat tgccgctaac
     8821 tggcgagcag aacctggctg gttggtttgc ggcagattgc gcggcacaca gagtaacgcc
     8881 tggcggctgt aatcaaacgg gcttggcaat agcaacgact ccgcctgttc tatcccaagc
     8941 cgcgaggtga aatgatgcag atcgtcgttc accgacagcg ttgctgaggt gaagatccag
     9001 ctaccgggct tttgcgccat taactctttg aatttatccg ccacgctgag cggcgtgaga
     9061 gccagagtaa aatggcgcga agtgcattcg taccagtagc tgtagcccgg ctgattgatc
     9121 tcttttagcc gcttcagccg tgtgcgatac aacgtggcgc gctcaaatgc cgcatccagc
     9181 aaggcggaac gccccagtga cagtttcgcc acgtcataac aaagttccaa ggtgtcatcg
     9241 agcagtaaaa atgcccgctg aatttgcgga ttagctaaca gctcacgcag gttaccgcga
     9301 taacctggct caccgagttg cagacgaaaa tcctgcgcac tctgggcaag acgatcagcg
     9361 cacttttgta actgctgggt gtcttttaat tcggtgcggt aggcgatggt gatgtctttt
     9421 gccaggtcga gcagttgtcg actggagagt gactgaccaa aatactggct ggcaatgtcc
     9481 ggtagctggt gggcttcgtc gaagatcatg acgtccgctt cagggatcaa ctcgccaaat
     9541 ccactctctt taaccaccat atccgccaga aagagatgat ggtttaccac caccacatcg
     9601 gcgtccatcg cttttttacg tgctttgacc acaaagcaat ctttatacat cgggcagtcg
     9661 ctgccaagac agttgtcgtt ggtgctggtg accagcggcc acgcctgtga atcttccgcc
     9721 acgctgacgc aggtgctgat atcaccatcg actgtttgat tagaccagga gcgcagcagg
     9781 atcacatcgc ttaagatttg taccggcaga tcgccccccg ccagcgcccg ctgttcgaga
     9841 cgttcgaggc agaggtagtt tgagcgccct ttcagcagcg ccacgttgcc cgtatatttc
     9901 aatgcctttg agactgttgg caaatcgcgg ctgtagagct gatcctgcaa cgcttttgag
     9961 ccggtcgaga taatgacttt ctttttcgcc cgcagcgcag gagccaggta agcgtaggtt
    10021 ttgcccgtac cggttcctgc ttccaccacc agcggctggc ctttttctat cgcctgggtg
    10081 acggctaccg ccatctgtcg ctgtggttct cgcgacttaa agcctggtat cgctttcgcc
    10141 agctgaccgt ctggtgcaaa atcgtccgtc acactacccc ctgttgattt gaacagggat
    10201 tatgtcagga tgagggcgct ttcgccagtt gaagtggtga cggcaacctc acattgtggc
    10261 agtctttgca gcacgaaaat ggaagtataa tgaggacaaa atgatgacta tcgttcgtat
    10321 cgatgctgaa gcccgctggt ctgatgtagt aatccacaac aacacgctct actacactgg
    10381 tgtaccggaa aacctcgacg ccgatgcctt tgagcaaacc gccaacacgc tggcacagat
    10441 tgacgccgtg ctggaaaaac agggcagcaa taaatcgagc attctggatg ccaccatttt
    10501 cctggccgat aaaaacgact tcgcggcgat gaataaagcg tgggatgcct gggttgtcgc
    10561 gggtcatgcg ccggtgcgct gcacggtaca agcgggtttg atgaacccga agtataaagt
    10621 tgaaattaag attgtggctg cggtgtaagc tttatcgaag caaaataagt cagacgataa
    10681 tttatcgata atactggtcg gttttacata aatcgaccag agaataagat attactgttg
    10741 atgcttctgc taattcatta tttatattta taatttcaat tttatctata aaactaacta
    10801 tataaataaa cataaccatg ccgttgtgtt cgccctttga ttagcaactc tggttttctt
    10861 tcttagctga acaatctggt tatagcgtgc gggctgaccg ttaattttct ccatcttact
    10921 ggataacaca ccatgagctg ctacctttaa aacacctgaa tgtcaacagg ttaactcgtg
    10981 ccatatcgtt ataatccctt tgatattaga tgcaaattaa ggtcatatat agccttatcg
    11041 ataacatggt taattttaag gataagaata tgcctgctgt aatagataaa gccctggatt
    11101 tcattggtgc catggatgta tcagcgccaa caccaagttc gatgaatgaa agcacggcga
    11161 agggaatctt taaatattta aaagaactgg gagtacccgc gagtgccgct gatattacgg
    11221 cgcgagctga ccaggaaggc tggaaccctg ggttcacgga aaaaatggtt ggatgggcaa
    11281 aaaaaatgga gacaggtgaa cgctctgtga ttaaaaatcc tgaatacttt tcaacatata
    11341 tgcaggaaga actaaaagca ctggtctgag ttaaatttat atcagcataa atgggtcagg
    11401 acgcttttaa tcacatataa aaagcgtcct ttttctcaca atcaaatgat catcggcttc
    11461 tataacgaaa tcgatacgga ttagtgtgtt actcgtcttc atcctcaaaa cgcgccacga
    11521 ttcgctcgcc ggagtggttg gcacgcagct cttctgccac cagcgcgatt gcctggccgc
    11581 tgctcatccc ttgtgccatc agttcctgga tccgctcgac agctttttgc tgctgttcat
    11641 gggtgagtga aggtaaacct gcaaacattg ttaactcctg ctaaattgtt ggcgctaatt
    11701 atttcatgct acccggcaca tagccagtag agtcaggact gatgaagacg ttatctcccg
    11761 ctgtgattac tttaccctgg cgtcaggacg ccgctgaatt ttatttctcc cgcttaagcc
    11821 acctgccgtg ggcgatgctt ttacactccg gctatgccga tcatccgtat agccgctttg
    11881 atattgtggt cgccgatccg atttgcactt taaccacttt aggtaaagaa accgttgtta
    11941 gtgaaagcga aaaacgcaca acgaccactg atgacccgct acaggtgctc cagcaggtgc
    12001 tggatcgcgc agacattcgc ccaacgcata acgaagattt gccatttcag ggcggcgcac
    12061 tggggttgtt tggctacgat ctgggccgcc gttttgagtc actgccagaa attgcggaac
    12121 aagatatcgt tctgccggat atggcagtgg gtatctacga ttgggcgctc attgtcgacc
    12181 accagcgtca tacagtttct ttgctgagtc ataatgatgt caatgcccgt cgggcctggc
    12241 tggaaagcca gcaattctcg ccgcaggaag atttcacgct cacttccgac tggcaatcca
    12301 atatgacccg cgagcagtac ggcgaaaaat ttcgccaggt acaggaatat ctgcacagcg
    12361 gtgattgcta tcaggtgaat ctcgcccagc gttttcatgc gacctattct ggcgatgaat
    12421 ggcaggcatt ccttcagctt aatcaggcca accgcgcgcc atttagcgct tttttacgtc
    12481 ttgaacaggg tgcaatttta agcctttcgc cagagcggtt tattctttgt gataatagtg
    12541 aaatccagac ccgcccgatt aaaggcacgc taccacgcct gcccgatcct caggaagata
    12601 gcaaacaagc agaaaaactg gcgaactcag cgaaagatcg tgccgaaaat ctgatgattg
    12661 tcgatttaat gcgtaatgat atcggtcgtg ttgccgtagc cggttcggta aaagtaccag
    12721 agctcttcgt ggtggaaccc ttccctgccg tgcatcatct ggtcagcact ataacggcgc
    12781 gactaccaga acagttacac gccagcgatc tgctgcgcgc agcttttcct ggtggctcaa
    12841 taaccggggc tccgaaagta cgggctatgg aaattatcga cgaactggaa ccgcagcgac
    12901 gtaatgcctg gtgcggcagc attggctatt tgagcttttg cggcaacatg gataccagca
    12961 ttactatccg cacgctgact gccattaacg gacaaatata ctgctctgcg ggcggtggaa
    13021 ttgtcgccga tagccaggaa gaagcggaat atcaggaaac ttttgataaa gttaataaga
    13081 tattacgcca actggagaag taagacgtgg aataccgtag cctgacgctt gatgattttt
    13141 tatctcgctt tcaacttttg cgcccgcaaa ttaaccggga aaccctaaat catcgtcagg
    13201 ctgccgtgtt aatccccatc gtccgtcgac cgcaaccggg gttgttgctg actcagcgtt
    13261 caattcatct gcgtaaacac gctggacaag tggcattccc tggaggtgca gtcgatgaca
    13321 cggacgcatc agttatcgcc gccgcgctgc gcgaagctga agaagaggtc gctataccgc
    13381 cttccgccgt tgaagttatc ggcgtgctgc cgcccgtcga tagcgtaact ggctatcagg
    13441 taaccccagt ggtcggcatt atcccgcccg atctgccgta tcgcgccagt gaagatgaag
    13501 tctcggcggt gtttgaaatg ccgctcgccc aggcattaca tctgggtcgt tatcaccctt
    13561 tagatatcta ccgccgtggc gattcacatc gggtatggct gtcctggtac gaacagtatt
    13621 ttgtatgggg aatgaccgca ggcataattc gtgagctggc gctgcaaatt ggtgtgaacc
    13681 cctgactata cttatcttta catctacaaa acactacttg agacaatcat cgcaatatta
    13741 gttaaatcgc ggtttttgat tagtttaatt catgtgaata gttaagccag tcgccgcgtt
    13801 ccctcttaca ctatgcgctg ttattagttc gttactggaa gtccagtcac cttgtcagga
    13861 gtattatcgt gattagtcta ttcgacatgt ttaaggtggg gattggtccc tcatcttccc
    13921 ataccgtagg gcctatgaag gcgggtaaac agttcgtcga tgatctggtc gaaaaaggct
    13981 tactggatag cgttactcgc gttgccgtgg acgtttatgg ttcactgtcg ctgacgggta
    14041 aaggccacca caccgatatc gccattatta tgggtcttgc aggtaacgaa cctgccaccg
    14101 tggatatcga cagtattccc ggttttattc gcgacgtaaa agagcgcgaa cgtctgctgc
    14161 tggcacaggg acggcatgaa gtggatttcc cgcgcgacaa cgggatgcgt tttcataacg
    14221 gcaacctgcc gctgcatgaa aacggtatgc aaatccacgc ctataacggc gatgaagtcg
    14281 tctacagcaa aacttattat tccatcggcg gcggttttat cgtcgatgaa gaacactttg
    14341 gtcaggatgc tgccaacgaa gtgagcgtgc cgtatccgtt caaatctgcc accgaactgc
    14401 tcgcgtactg taatgaaacc ggctattcgc tgtctggtct cgctatgcag aacgaactgg
    14461 cgctgcacag caagaaagag atcgacgagt atttcgcgca tgtctggcaa accatgcagg
    14521 catgtatcga tcgcgggatg aacaccgaag gtgtactgcc aggcccgctg cgcgtgccac
    14581 gtcgtgcgtc tgccctgcgc cggatgttag tttccagcga taaactgtct aacgatccga
    14641 tgaatgtcat tgactgggta aacatgtttg cgctggcagt taacgaagaa aacgccgccg
    14701 gtggtcgtgt ggtaactgcg ccaaccaacg gtgcctgcgg tatcgttccg gcagtgctgg
    14761 cttactatga ccactttatt gaatcggtca gcccagacat ctatacccgt tactttatgg
    14821 cagcgggcgc gattggtgca ttgtataaaa tgaacgcctc tatttccggt gcggaagttg
    14881 gttgccaggg cgaagtgggt gttgcctgtt caatggctgc tgcgggtctt gcagaactgc
    14941 tgggcggtag cccggaacag gtttgcgtgg cggcggaaat tggcatggaa cacaaccttg
    15001 gtttaacctg cgacccggtt gcagggcagg ttcaggtgcc gtgcattgag cgtaatgcca
    15061 ttgcctctgt gaaggcgatt aacgccgcac ggatggctct gcgccgcacc agtgcaccgc
    15121 gcgtctcgct ggataaggtc atcgaaacga tgtacgaaac cggtaaggac atgaacgcca
    15181 aataccgcga aacctcacgc ggtggtctgg caatcaaagt ccagtgtgac taatacttct
    15241 tactcgccca tctgcaacgg atgggcgaat ttatacccgc tttctcgtct gctgtaatat
    15301 tccccactac acttccactg ttgcgtcagg cgtttgtcgc catacgctta cagggtggcc
    15361 cgcatgcaaa aagcacaacg gatcattaaa acctatcgcc gtaatcgaat gattgtttgt
    15421 acgatttgcg cactcgttac gctcgcttcg accctgagcg tgcgatttat ttcacagcgt
    15481 aacttaaatc aacaacgggt agtacaattc gccaatcacg ctgtagagga attagataaa
    15541 gtactgcttc ccctacaggc aggtagcgaa gtcttgcttc cgctgattgg tctgccctgc
    15601 tctgtcgccc atttgccatt acgtaaacag gcggcaaaac tccaaactgt gcgatccatt
    15661 ggcctggtgc aagacggcac actttattgc tccagcattt ttggttatcg caatgtgccc
    15721 gtcgtggaca ttctggctga acttcctgca ccgcaaccac ttttacgcct gacgatcgac
    15781 cgtgccctga ttaaaggcag tccggttttg attcaatgga cgcctgcagc gggcagtagc
    15841 aatgctgggg tcatggagat gattaacatc gacttactga cggcaatgct ccttgagcca
    15901 caactgccgc aaatcagtag cgccagcctg acggtggaca aacggcattt gctctatggt
    15961 aatgggctgg tagattccct tccgcaacct gaagacaatg aaaactatca ggtttcttcg
    16021 caaagctttc cttttaccat taacgtaaat ggtccggggg ctacggcgct ggcatggcac
    16081 tatcttccaa cacaattacc gctggcggtg ctgctaagtt tactggtggg ctatatcgcc
    16141 tggctggcga ccgcttaccg gatgagcttt tcccgcgaaa ttaatctggg cctggcgcaa
    16201 catgagttcg aattgttctg tcagcctttg cttaatgcgc gcagccagca atgtattggt
    16261 gtagagattt tgctgcgctg gaataatccg cgtcagggct ggatttcacc ggatgtgttt
    16321 attcctatcg cggaagaaca tcatttaatt gtgccactga cccgctatgt gatggcagaa
    16381 accattcgtc agcgccatgt tttcccgatg agtagtcagt ttcatgttgg cattaacgtc
    16441 gcacccagcc attttcgccg tggtgtgctg ataaaagatc tcaatcagta ctggtttagc
    16501 gctcacccga ttcagcaact gatcctcgaa atcaccgaac gcgatgcctt actggatgtt
    16561 gattatcgga ttgcccgcga gctgcatcgt aaaaacgtca aactggcgat tgatgacttc
    16621 ggcaccggca acagctcgtt ttcctggctt gaaacattac gtcctgacgt gctgaaaatt
    16681 gataagtcat ttaccgcagc tataggttct gacgcggtta actcgacggt gaccgatatc
    16741 atcatcgcgc tggggcaaag actgaatatt gaactggtgg cggagggcgt ggaaacgcaa
    16801 gaacaggcga agtatttgcg ccgtcatggc gtgcatattt tgcaagggta tttgtacgca
    16861 cagccgatgc cgctacgtga ttttcccaaa tggctggcgg gcagccaacc gccgcccgcc
    16921 cggcataatg gacatatcac gcccattatg tcgttacgtt aacgcttact catcttcatc
    16981 gtgcgccggt tgctctttaa caatgcgaac cagatcaaca cgataatcat tggcttcaag
    17041 gatggtgata tgcagtggcc ctacatcaat cacatcgccc acacggggaa tgtgaccatt
    17101 tgccgagatc acgaggcccg cgaccgtcgc gatatcatcg tcatcggcaa ggtgctcaac
    17161 atcaagcgcc tgctgcaagg catgcaaatc tgtaccgcct tttaccagcc agccgttacc
    17221 atcggtaatg atttccggcg tttcgtcagc gtccgggaat tcacccgcaa tggcttccag
    17281 cacatccagc ggcgtgacca agccttgtac cacaccaaac tcgttggtca cgataacaaa
    17341 gctcccgcga gcgcgacgca gcacgcccag caggttgatc ggatcgaggg tttccgggac
    17401 gataatcgcc ggagacgccg aagcaatcgc cgccacatca acgccctctt ccagcgccac
    17461 cagcagttct ttagcacgta cgataccgat gatttcatcc agttcaccgc gacataccgg
    17521 gaacagactg tgcggtgaag agagcagttg ctcgcggatt tcatcgaccc cgagattagc
    17581 gtcaacccag cttatttcac cgcgcggcgt catgatcccg cgcagagaac gcgacgccag
    17641 cgtcagtacg ccgttaatca tgtaccgttc ttcttcggca aatgcacctt ccgggatcgg
    17701 catcgggtta tcggcatcgt gctgaacatt ggcctgacgt ttcccgccca tcaaacgcag
    17761 gatggcatcg gcagtacgcg ctcgcagcgg caaagtcgac tggtgacgaa taaagttgcg
    17821 acgcgcaatc tggttaaaca cttcgataat gatcgagaag ccaatcgcgg catacaggta
    17881 acctttcgga atgtggaaac cgaaaccttc tgccaccaga ctcagaccaa tcattaacag
    17941 gaagctcaga cagagcacca ccaccgtggg gtgctggtta acgaatcgcg tcagcggttt
    18001 cgatgccagc aacataaccg ccatcgcaat cactaccgcc gccatcatca ccggcagatg
    18061 gttaaccatc cctactgcag taattaccgc atccaacgag aagacggcgt caaggatgac
    18121 aatctgtgtg acgaccaccc agaaactggc gtagccttta ccgtggccgg aatcatgatc
    18181 gcggttttcc agccgttcat gcagttcggt tgttgctttg aacagcaaga atatcccccc
    18241 gaacaacata atcagatcgc gtccggagaa ggagaaatcc atgacggtaa atagcggttt
    18301 ggtcagcgtg accatccatg aaatcagcga cagcagcccc agacgcataa tcagcgccag
    18361 tgataacccc agcaaacgcg ctttatcgcg ttgttttggc ggcagtttgt cagcaagaat
    18421 ggcgatgaag accaggttat cgatacccag cacaatttcg agaacaacaa gcgtgagtag
    18481 ccccgcccaa attgaggggt ccattaagaa ttccatgaca agctcctgct taaggaatag
    18541 ctattcgacg ccagaaataa tgcaggcgta acgacaaaat gcaaacgaaa ggtgcggcat
    18601 agagtgccag aaaggcaggc gttaaaaggc ctgatgctga aatgacgtcg gtgacgatcc
    18661 atactgcggg ctactgccct atactccatg gttgttaaac gggagttaaa catatcagag
    18721 acgcctctga tttggcaaag atttaccttc ctttgcaaac gaatgtgaca aggatatttt
    18781 acctttcgaa atttctgata atcgaaagtt aaattacgga tcttcatcac ataaaataat
    18841 tttttcgata tctaaaataa atcgcgaaac gcaggggttt ttggttgtag cccttatctg
    18901 aatcgattcg attgtggacg acgattcaaa aatacatctg gcacgttgag gtgttaacga
    18961 taataaagga ggtagcaagt gaccattgct attgttatag gcacacatgg ttgggctgca
    19021 gagcagttgc ttaaaacggc agaaatgctg ttaggcgagc aggaaaacgt cggctggatc
    19081 gatttcgttc caggtgaaaa tgccgaaacg ctgattgaaa agtacaacgc tcagttggca
    19141 aaactcgaca ccactaaagg cgtgctgttt ctcgttgata catggggagg cagcccgttc
    19201 aatgctgcca gccgcattgt cgtcgacaaa gagcattatg aagtcattgc aggcgttaac
    19261 attccaatgc tcgtggaaac gttaatggcc cgtgatgatg acccgagctt tgatgaactg
    19321 gttgcgctgg cagtagaaac aggccgtgaa ggcgtgaaag cactgaaagc caaaccggtt
    19381 gaaaaagccg cgccagcacc cgctgccgca gcaccaaaag cggctccaac tccggcaaaa
    19441 ccaatggggc caaacgacta catggttatt ggccttgcgc gtatcgacga ccgtctgatt
    19501 cacggtcagg tcgccacccg ctggaccaaa gaaaccaatg tctcccgtat tattgttgtt
    19561 agtgatgaag tggctgcgga taccgttcgt aagacactgc tcacccaggt tgcacctccg
    19621 ggcgtaacag cacacgtagt tgatgttgcc aaaatgattc gcgtctacaa caacccgaaa
    19681 tatgctggcg aacgcgtaat gctgttattt accaacccaa cagatgtaga gcgtctcgtt
    19741 gaaggcggcg tgaaaatcac ctctgttaac gtcggtggta tggcattccg tcagggtaaa
    19801 acccaggtga ataacgcggt ttcggttgat gaaaaagata tcgaggcgtt caaaaaactg
    19861 aatgcacgcg gtattgagct ggaagtccgt aaggtttcca ccgatccgaa actgaaaatg
    19921 atggatctga tcagcaaaat cgataagtaa cgtattgtgt tgattatcat tcagttttca
    19981 cacttaagtc ttacgtaaac aggagaagta caatggagat taccactctt caaattgtgc
    20041 tggtatttat cgtagcctgt atcgcaggta tgggatcaat cctcgatgaa tttcagtttc
    20101 accgtccgct aatcgcgtgt accctggtgg gtatcgttct tggggatatg aaaaccggta
    20161 ttattatcgg tggtacgctg gaaatgatcg cgctgggctg gatgaacatc ggtgctgcag
    20221 ttgcgcctga cgccgctctg gcttctatca tttctaccat tctggttatc gcaggtcatc
    20281 agagcattgg tgcaggtatc gcactggcaa tccctctggc cgctgcgggc caggtactga
    20341 ccatcatcgt tcgtactatt accgttgctt tccagcacgc tgcggataag gctgctgata
    20401 acggcaacct gacagcaatt tcctggatcc acgtttcttc tctgttcctg caagcaatgc
    20461 gtgtagctat tccggccgtc atcgttgcgc tgtctgttgg taccagcgaa gtacagaaca
    20521 tgctgaatgc gattccggaa gtggtgacca atggtctgaa tatcgccggg ggcatgatcg
    20581 tggtggttgg ttatgcgatg gttatcaaca tgatgcgtgc tggctacctg atgccgttct
    20641 tctacctcgg cttcgtaacc gcagcattca ccaactttaa cctggttgct ctgggtgtga
    20701 ttggtactgt tatggcagtg ctctacatcc aacttagccc gaaatacaac cgcgtagccg
    20761 gtgcgcctgc tcaggcagct ggtaacaacg atctcgataa cgaactggac taacaggtga
    20821 gcgaaatggt tgatacaact caaactacca ccgagaaaaa actcactcaa agtgatattc
    20881 gtggcgtatt cctgcgttct aacctcttcc agggttcatg gaacttcgaa cgtatgcagg
    20941 cactgggttt ctgcttctct atggtaccgg caattcgtcg cctctaccct gagaacaacg
    21001 aagctcgtaa acaagctatt cgccgtcacc tggagttctt taacacccag ccgttcgtgg
    21061 ctgcaccgat tctcggcgta accctggcgc tggaagaaca gcgtgctaat ggcgcagaga
    21121 tcgatgacgg tgctatcaac ggtatcaaag tcggtttgat ggggccgctg gctggtgtag
    21181 gcgacccgat cttctgggga accgtacgtc cggtatttgc agcactgggt gccggtatcg
    21241 cgatgagcgg cagcctgtta ggtccgctgc tgttcttcat cctgtttaac ctggtgcgtc
    21301 tggcaacccg ttactacggc gtagcgtatg gttactccaa aggtatcgat atcgttaaag
    21361 atatgggtgg tggcttcctg caaaaactga cggaaggggc gtctatcctc ggcctgtttg
    21421 tcatgggggc attggttaac aagtggacac atgtcaacat cccgctggtg gtttcgacca
    21481 tcaccgggca ggatggacag acccgtgtca ccaccgtgca aactattctt gaccagttaa
    21541 tgccaggcct tgtaccactg ctgctgacat ttgcttgtat gtggctgctg cgcaaaaaag
    21601 ttaacccgct gtggatcatc gttggcttct tcgtcatcgg tatcgctggt tacgcttgcg
    21661 gcctgctggg actgtaagac tgttgtacac taccggggcc ttttggcccc gtttttttat
    21721 ctggaggatt aatgacaatc acggacctgg tactgattct tttcatcgcc gcactcctgg
    21781 ccttcgcgat ctacgatcag ttcatcatgc cccgccgtaa cggccccacc ctgctggcaa
    21841 ttcctttgct ccggcgtggt cgcatcgata gcgttatctt cgtcggattg attgtcattc
    21901 ttatctataa caacgtcacg aatcatggcg cgctaataac aacatggtta ttaagtgcac
    21961 tggctctgat gggtttttat atattctgga tccgcgttcc gaagatcatc tttaaacaaa
    22021 aaggtttttt cttcgccaat gtctggattg aatatagccg aatcaaagcg atgaacttgt
    22081 cggaagatgg cgtgttggtg atgcaattag aacagcgtcg gctgttaatc cgcgttcgaa
    22141 atatcgacga tctggaaaaa atttataagc ttctcg
//
LOCUS       DADLSA010000053        21738 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.053, whole genome shotgun sequence.
ACCESSION   DADLSA010000053 DADLSA010000000
VERSION     DADLSA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.053"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..798)
                     /locus_tag="JN691_002073"
     CDS             complement(<1..798)
                     /locus_tag="JN691_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY7243523.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHG
                     AQLLDRLLEPGDIVGFSWGRAVSALVENLPQAGQSQQLICVPIIGGPSGKLESRYHVN
                     TLTYSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSP
                     AIRDGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEK"
     gene            1146..2018
                     /gene="rluF"
                     /locus_tag="JN691_002074"
     CDS             1146..2018
                     /gene="rluF"
                     /locus_tag="JN691_002074"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAY7243524.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(2151..2423)
                     /locus_tag="JN691_002075"
     CDS             complement(2151..2423)
                     /locus_tag="JN691_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAY7243525.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLNNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(2676..4025)
                     /gene="lysC"
                     /locus_tag="JN691_002076"
     CDS             complement(2676..4025)
                     /gene="lysC"
                     /locus_tag="JN691_002076"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAY7243526.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQAVQKLHSNLFE"
     gene            4550..6199
                     /gene="pgi"
                     /locus_tag="JN691_002077"
     CDS             4550..6199
                     /gene="pgi"
                     /locus_tag="JN691_002077"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAY7243527.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQGLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            6661..6903
                     /locus_tag="JN691_002078"
     CDS             6661..6903
                     /locus_tag="JN691_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243528.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            7017..7655
                     /locus_tag="JN691_002079"
     CDS             7017..7655
                     /locus_tag="JN691_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY7243529.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            7652..8389
                     /locus_tag="JN691_002080"
     CDS             7652..8389
                     /locus_tag="JN691_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY7243530.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRVPQ"
     gene            8389..10485
                     /locus_tag="JN691_002081"
                     /pseudo
     CDS             8389..10485
                     /locus_tag="JN691_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
     gene            complement(10532..10810)
                     /locus_tag="JN691_002082"
     CDS             complement(10532..10810)
                     /locus_tag="JN691_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243531.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            10811..10954
                     /locus_tag="JN691_002083"
     CDS             10811..10954
                     /locus_tag="JN691_002083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243532.1"
                     /translation="MFQRIIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            11080..11490
                     /gene="psiE"
                     /locus_tag="JN691_002084"
     CDS             11080..11490
                     /gene="psiE"
                     /locus_tag="JN691_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HAY7243533.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(11534..13009)
                     /gene="xylE"
                     /locus_tag="JN691_002085"
     CDS             complement(11534..13009)
                     /gene="xylE"
                     /locus_tag="JN691_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HAY7243534.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(13381..14271)
                     /gene="malG"
                     /locus_tag="JN691_002086"
     CDS             complement(13381..14271)
                     /gene="malG"
                     /locus_tag="JN691_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HAY7243535.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(14286..15830)
                     /gene="malF"
                     /locus_tag="JN691_002087"
     CDS             complement(14286..15830)
                     /gene="malF"
                     /locus_tag="JN691_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HAY7243536.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(15984..17174)
                     /gene="malE"
                     /locus_tag="JN691_002088"
     CDS             complement(15984..17174)
                     /gene="malE"
                     /locus_tag="JN691_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HAY7243537.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            17539..18654
                     /gene="malK"
                     /locus_tag="JN691_002089"
     CDS             17539..18654
                     /gene="malK"
                     /locus_tag="JN691_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HAY7243538.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            18726..20066
                     /gene="lamB"
                     /locus_tag="JN691_002090"
     CDS             18726..20066
                     /gene="lamB"
                     /locus_tag="JN691_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HAY7243539.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            20218..21138
                     /gene="malM"
                     /locus_tag="JN691_002091"
     CDS             20218..21138
                     /gene="malM"
                     /locus_tag="JN691_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HAY7243540.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPSLGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            21367..>21738
                     /locus_tag="JN691_002092"
     CDS             21367..>21738
                     /locus_tag="JN691_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243541.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVTGLNRDN
                     FIYISDIFLNIKQRNEKNHAINMLREVSISNDTISVKFYRNEKIECACNFLMDKDAQG
                     YIDLSDFDLTNCHFKGDVISKV"
BASE COUNT         5455 a         5539 c         5587 g         5157 t
ORIGIN      
        1 tttttcgctc atattcgttt caaccattgc gccgtgaata tcaaaaaagc gcgagcaaat
       61 atcgccagca acctggcggg cattcaggtc gtcactctct tcaccaccat aaaacgcatg
      121 ccagttagcg ccgtcgcgaa tggccggtga gccaattccc accagggcga catccagatt
      181 atcccagtag gcagagatgg ttttaaagtg ctgagactgc atgatcccat tacgaattaa
      241 tgggttatcc agcagagccg gaaaatccgc gagatgcgat tcccctttca gcttcgctgc
      301 cgcgctgtag gttaatgtgt ttacatgata gcggctttcg agtttaccgg acggtccgcc
      361 aataattggt acgcagatta actgctgcga ttgccccgcc tgcggcaagt tttcaaccag
      421 tgcgctcacc gcgcgacccc aggaaaaacc gacaatatcg ccaggctcca gcaagcgatc
      481 cagcagttgc gcgccgtgta accccatcat cgccagttga gtctcttcat cctcatcatt
      541 tcccgacacc accacaacgt ctttcaggcc aaacttttgc ttcagttgct gctccagcca
      601 gagattttcg ttgtagtcat agtagtcata gttgatggcg atggtgacca ttccctgatc
      661 gcggcctcgt ttaagcaagc ggctgatggt ggtgcggtaa atacccagtt cgcgcgcgat
      721 ttgcgcctgc gtcatatcct gttcgtaata gagttgggca atcttcacaa tcaaacggat
      781 atcgtcactg ttttccattg ccgcctcctg gttctgcaca tttgtgcaaa tacgctttct
      841 caccgcttga tctgatataa ccccatcatg agggcctttc aaagcgattt ttcactcttt
      901 gcatggggag aaatagcgct ttctcttgca catttgtttc tctgcgtagt agtcatcagc
      961 acaggtgtgc agcgattcgt aatatgttga ttatccttat tttgccaggc aaatttgcat
     1021 cgtacttccg gagacacttt tgtgataaaa ggcgtggcag atcacattta atttcgacgt
     1081 tgccgcataa ccctgttttt ccccgctaag aggtacggta tattccgcgt tttacaggag
     1141 aatttatgct gcccgactca tcagtccgtt taaataaata catcagcgaa agcggaattt
     1201 gctcacgccg cgaagcggat cgctatatcg agcaaggcaa tgtgttcctt aatggcaagc
     1261 gagccaccat tggcgatcag gtgaaacccg gcgacattgt gaaagtaaac ggtcagttga
     1321 ttgagcctcg ggaagccgaa gatttggtac ttatcgccct gaacaagccc gtcggtattg
     1381 tcagcaccac cgaagatggc gagcgcgata acattgtcga tttcgttaac cacagcaaac
     1441 gcgtgttccc gattggccgc ctggataaag actcccaggg gctgattttc ctcaccaatc
     1501 acggcgatct ggtgaataag atcctccgtg cgggtaacga tcatgagaaa gagtatctgg
     1561 tgacggtcga taaaccgatt accgatgaat ttattcgtgg tatgggggcg ggggtgccga
     1621 tcctcgggac ggtgacgaaa aagtgcaaag tcaaaaaaga agcgccgttt gtcttccgca
     1681 ttaccctagt gcaggggctg aaccgtcaga tccggcgcat gtgcgagcat ttcggctatg
     1741 aagtgaaaaa gctggaacgt acgcgcatta tgaacgtcag cttaagcggc attccgctgg
     1801 gggagtggcg cgatttaacc gacgatgagt taatcgacct ctttaagctc atcgaaaatt
     1861 cctcttccga ggcaaaacct aaagcgaagg ccaaaccgaa aacagcgggc atcaaacgtc
     1921 cagtcgttaa gatggaaaaa acggcggaaa aaggcggtcg cccggcgtcc aacggtaagc
     1981 gttttacctc gccggggcgt aaaaagaagg gacgctgatt aacgcctcag actgatgaaa
     2041 acgtccaaaa agagctcgga cagtcccctc gccccttcgg ggagagggtt agggtgaggg
     2101 gaagattgtg tccacacaga cgagtgaaaa tctcaggcgt tgattgttca ttagcgcctg
     2161 ccgcgcgtcg acgtatttgc tgaccagcta aatgacgctt caggatccgg tttataagcc
     2221 tgctttttct tcaattggcg ggcttttttc gcttcagcct cacgctcgac cattaattta
     2281 tcgatgtact ctttcttaat gctgttggtt tcggagttgt tcagtacgcg accatgcgca
     2341 atccgcgccc gatccagcag tgttttaagt tcacgctgct cgcgttcagt catctctttt
     2401 tgggtaatac gggggagtgc catgatggtg ccctcagtga gccagagagt tcagtgtacg
     2461 ggaattcgtt tgcgagcaga actgataata ttcattttgc cttctcttaa ctatttatct
     2521 ctttcgataa ttaattagag atgcagataa aaaaattaaa ggcaattatt ctccgataaa
     2581 gacgttggtt aacagagtac aggctcgttt attgatgagc atagtgacaa gaaaatcaat
     2641 acggcccgaa atatagcttc caggccatac agtatttact caaacaaatt actatgcagt
     2701 ttttgcaccg cctgctcggc atcttcgccg ggcaccagga agcacaggtt atggctggat
     2761 gcgccgtaac aaatcatgcg aatgttgaac ggttccagta cgccgaatac ctctttgcca
     2821 acgccgcagg cttttgacag gtcattgcca atcaacgcga ccagcgccag accttcttcc
     2881 acctccaccc ggcacagtgc ggaaagctcc atcagcagag attgcgtcag caacgtatcg
     2941 ccagtggagg ttgaaccggt ggtatcaagg gttaatgcca cgctcacttc tgacgtggtg
     3001 attaagtcta ccgaaatatt atgccgcgcg aggatgccga aaacttccgc gaggaagccg
     3061 cgagaatgca gcatattcag gctgtgcaaa gtgagcagag tctgattgcg acgaagcgcc
     3121 agcgcgcgga acagcggcgg attttcagtt ttattgcaca ccagcgtacc acctgcgcgt
     3181 gggtctttgc tggagccgac aaagaccggg atatcgctgc gtactgcggg tagcaacgtt
     3241 gccggatgca gtacttttgc accaaaagtt gccatctctg ccgcttcggc aaacgcgatt
     3301 tcatcaatgc gttttgctgc ggaaactacg cgtggatcgg tggtgtagat gcccgggaca
     3361 tcggtccaga tatcaacacg agatgcgtgt aaagcctccg ccagcaaggc tgccgtataa
     3421 tcgctgcctc cacggccaag cgtcgttgta cgacctttat tttcgctacc gataaatccc
     3481 tgggtgatca ctaagccttc attgaggcgt gggagcagct gcagcgcggc cagttccgcc
     3541 agcgcggcta catctggctc tgcacgacca aatcggtcgt tggtacgcat cactttacgt
     3601 acatcaaacc actgtgcctg aacatcgcgt tcgcgcagga tttcgacaaa cagcagggtc
     3661 gacatcagct cgccgtggct gaccagctca tctgtcagcg ccggagacgt tgccagcgcc
     3721 gccgcttctg ccagaacagt aatgttctcc agcagacgtt caatctcttc acggataacg
     3781 ttcgggtaac gcagacgttc cagaatggca aactggatgt tgcgaatagc gtcgagtttt
     3841 tcgaatcgct cgccaggttc cagcccttcc gctaaagcga ccagcagatt agtgatacca
     3901 gcagaagccg agaggactac taaacgcacg ttggcatcag aaagcacaat atcagcgctg
     3961 cggttcatgg cgtcaaaatc agctacgctg gtaccgccaa atttggagac aacaatttca
     4021 gacataacta cctcgtgtca ggggatccat tttcagcctt ggcacaaggg aagagcggaa
     4081 gacgggtggg cgcagagcga tacttcgcta ctattttcac ccagaagtgc tccaccactt
     4141 gcgaaacgcc cgactgcgaa cgcttctggt gacaacccag gggattcagc ccctgtagcc
     4201 gatgatgaac gtggccagcc gttcaatcac ctcggcgatg caccccctca ggtgttatca
     4261 caggactggc tcctccaaca ccgttacttg ggcaacgcgc ctcttctggc ctgcgctagc
     4321 gcaggtagta catttataaa taaagggtga gcggggcggt tgtcaacgac ggggtcatgc
     4381 ggatttttca tccactcctg gcggtcagta gttcagctaa taaatgcttc actgcgctaa
     4441 gggtttacac tcaacattac actaacggca ctaaaaccat cacatttttc tgtgactggc
     4501 gctacaatct tccaaagtca caattctcaa aatcagaaga gtattgctaa tgaaaaacat
     4561 caatccaacg cagaccgctg cctggcaggc actacagaaa cacttcgatg aaatgaaaga
     4621 cgttacgatc gccgatcttt ttgctaaaga cggcgatcgt ttttctaagt tctccgcaac
     4681 cttcgacgat cagatgctgg tggattactc caaaaaccgc atcactgaag agacgctggc
     4741 gaaattacag ggtctggcga aagagtgcga tctggcgggc gcgattaagt cgatgttctc
     4801 tggcgagaag atcaaccgca ctgaaaaccg cgccgtgctg cacgtagcgc tgcgtaatcg
     4861 tagcaatacc ccgattctgg ttgatggcaa agatgttatg ccagaagtca acgcggtgct
     4921 ggagaagatg aaaaccttct cggaagcgat tatctccggt gagtggaagg gttataccgg
     4981 caaagcaatc actgacgtag tgaacatcgg gatcggcggt tctgacctcg gcccgtacat
     5041 ggtgaccgaa gcactgcgtc cgtacaaaaa ccacctgaac atgcactttg tttctaacgt
     5101 cgatgggact cacatcgcgg aagtgctgaa aaaagtaaac ccggaaacca cgctgttcct
     5161 ggtagcatct aaaactttca ccactcagga aaccatgacc aacgcccata gcgcgcgtga
     5221 ctggttcctg aaagcggcag gtgatgaaaa acacgttgcg aaacattttg cggcgctctc
     5281 aaccaatgcc aaagccgttg gcgagtttgg tattgatact gccaacatgt tcgagttctg
     5341 ggactgggtc ggcggccgtt actctttgtg gtcagcgatt ggcctgtcga ttgttctctc
     5401 catcggcttt gataacttcg ttgaactgct ttctggcgca cacgcaatgg acaagcattt
     5461 ctccaccacg cctgccgaga aaaacctgcc tgtcctgctg gcgctgattg gcatctggta
     5521 caacaatttc tttggtgcgg aaactgaagc gattctgccg tatgaccagt atatgcaccg
     5581 tttcgcggcg tacttccagc agggcaatat ggagtccaac ggtaagtatg ttgaccgtaa
     5641 cggtaacgtt gtggattacc agactggccc gattatctgg ggtgaaccag gcactaacgg
     5701 tcagcacgcg ttctaccagc tgatccacca gggaaccaaa atggtaccgt gcgatttcat
     5761 cgccccggct atcacccata acccgctctc tgatcaccac cagaaactgc tgtctaactt
     5821 cttcgcccag accgaagcgc tggcgtttgg taaatcccgc gaagtggttg agcaggaata
     5881 tcgcgatcag ggtaaagatc cggcaacgct tgactacgtg gtgccgttca aagtgttcga
     5941 aggtaaccgc ccgaccaact ccatcctgct gcgcgaaatc actccgttca gcctgggtgc
     6001 gttgattgcg ctgtatgagc acaaaatctt tactcagggc gtgatcctga acatcttcac
     6061 cttcgaccag tggggcgtgg agctgggtaa acaactggca aaccgtattc tgccagagct
     6121 gaaagatgat aaagaaatca gcagccacga tagctcgacc aatggtctga ttaaccgcta
     6181 taaagcgtgg cgcggttaat catcatgcgt gcctgatgcc gatatgtagg ccggataagg
     6241 cgtttacgcc gcatccggca accggagcct gatgccaata tcgacattaa tgcatagtat
     6301 gaggagttaa ataaccttaa aaacaatgcc gacaataagt cggcattgtt ttatcagata
     6361 aatccccttg tctgtgattt aacggaaatc ataccgtgag gttaatccta aaatagattt
     6421 ttaatcgttg tttatttcgg agaatacgca gattaattac ttttgttttt attttaagtt
     6481 tatgattttt attgttattt aaatataagt tgaaacttat atttgatatt cattccaatt
     6541 atcctaaaac tccatcgcta attccccgcg ccgtaattcg catgctttag ttgtgtatac
     6601 tcgatcccgc ccgaaatgtt tttgggtaaa tctccattca ttcaatgaag ggaaattgtt
     6661 atgaaaaaag ttctgtatgg catttttgcc atatctgcgc ttgcggcgac ttctgcgtgg
     6721 gctgcacctg tacaggtggg cgaagcggca gagtcggcag caacgtcggt ttcggcgggg
     6781 agttcctccg cgaccagcgt cagcaccgta agctcggcgg tgggtgtcgc gctcgcggca
     6841 accggtggcg gtgatggttc taataccggg accaccacaa ccacgaccac cagtacccag
     6901 taataaagta tgtatcccca aaataatttg agtcattgca tctgtggctt gaagtatgaa
     6961 gggatttacc ataaccacac tccggtgtgg ttattctgcc cctctggaga agagtcgtga
     7021 agcgacctgc actcattctt atctgcctgc tattacaggc ctgttcagcc acgactaaag
     7081 agctgggcaa ttcactgtgg gacagtctgt tcggcacgcc aggcgtacag ctgacggacg
     7141 atgatattca aaatatgccc tacgccagcc agtacatgca gcttaatggc gggccgcaat
     7201 tgtttgtggt gctggccttc gctgaagacg gacaacaaaa atgggtcact caggatcaag
     7261 ccactcttgt cacacaacat ggtcgtctgg tgaagacttt gcttggcggc gacaacctga
     7321 ttgaagtgaa taaccttgcc gccgacccgc tgattaaacc cgcacaaatt gttgatggtg
     7381 caacatggac gcgcacgatg ggctggaccg agtaccagca ggtacgctac gccaccgcac
     7441 gctcagtctt caaatgggat ggcaccgata ccgtcaaggt cggcagcgat gaaaccctgg
     7501 ttcgcgtgct ggacgaagaa gtctccaccg accaggcgcg ctggcataac cgctactgga
     7561 tcgacagcga agggcaaatt cgccagtcgg aacagtatct cggcgcggat tatttcccgg
     7621 tgaaaaccac tctcatcaag gcggcaaaac aatgattaaa caaactattg tcgcgttgct
     7681 tttgagtgtg ggagcgtcat cggtcttcgc ggcaggaacc gtcaaggtgt tcagcaatgg
     7741 cagcagtgag gccaaaacgc taacgggcgc agagcattta atcgatctgg tagggcaacc
     7801 tcgactggca aacagctggt ggcctggtgc ggtgattagc gaagagctgg caacggcggc
     7861 agcattgcgt cagcagcagg cgttgctgac acggctggca gaacagggcg cagattccag
     7921 caccgacgat gccgccgcca ttaacgcctt acgccagcaa atccaggcat tgaaagtgac
     7981 aggcaggcaa aaaatcaatc ttgatcccga tatcgtccgc gttgccgaac gcggtaaccc
     8041 gccgttgcag ggcaactaca cgctgtgggt cggaccaccg ccgtccacgg tcacgttgtt
     8101 cgggcttatc agccatcctg gcaatcagcc attcactcca ggtcgcgacg tggcgagcta
     8161 tctctctgac cagagcctgc tcagcggtgc ggatcgcagc tacgcgtggg tcgtttaccc
     8221 ggacggacgc acgcaaaaag cgccggtggc ttactggaac aagcgtcacg tagagccgat
     8281 gcccggcagc attatttatg ttggcctcgc agactccgtc tggagtgaga cgcctgatgc
     8341 ccttaacgcc gacattcttc agactctgac gcagcgggta ccccaataat gaaaaaaaga
     8401 catctgctta gcttactggc gctgggcatt agcacagctt gctacggcga aacatatcct
     8461 gcgcccattg gtccgtcgca gtcggatttc ggtggcgtag gattattaca aactcccacc
     8521 gcgcgtatgg cacgggaagg ggagttgagt ctgaactatc gcgataacga tcagtaccgt
     8581 tattactcag cttcagtgca actcttcccg tggctggaaa caacgctgcg ctacaccgac
     8641 gtgcgcaccc ggcagtacag cagcgtcgaa gcgttctctg gcgatcaaac gtataaagat
     8701 aaagccttcg atctcaaact gcgtttgtgg gaagagagtt actggctgcc gcaagtggcg
     8761 gttggtgcgc gggatatcgg cggtacgggg ctgtttgatg cggaatatct tgttgccagc
     8821 aaagcctggg ggccgttcga ttttacgctc ggcctgggct ggggatatct gggcaccagc
     8881 ggtaatgtaa aaaatccgct ctgttcagcc agtgataaat attgctatcg cgataacagc
     8941 tacaaacagg cgggatctat cgacggtagc cagatgttcc acggtcctgc ctcactgttt
     9001 ggcggcgtgg aataccagac gccttggcaa ccgctgcgtc tgaaactgga gtatgaaggt
     9061 aataattatc agcaggattt tgccgggaag ctggagcaaa aaagtaagtt taacgtcggg
     9121 gcgatttatc gcgttatcga ttgggccgac gttaacctta gctatgaacg tggcaacacc
     9181 tttatgtttg gcgtcacgct gcgcaccaac tttaacgatc tgcgcccgtc ctacatcgat
     9241 aacgcccgcc cgcaatatca accgcagccg caggatgcca ttttgcagca ttcggtggtg
     9301 gcgaatcagt taacgttgca gaaatacaac gccggacttg ccgatccgca gatccaggcg
     9361 aaaggcgata cgctgtatgt taccggcgag caggtgaaat atcgtgattc gcgcgaaggg
     9421 atcatccgtg ctaatcggat cgtgatgaac gatctgccgg atgggatcaa aacgatccgc
     9481 attacggaaa atcgccttaa catgccgcaa gtgacgacgg aaaccgatgt cgccagcctg
     9541 aaaaatcatc tcgccggaga gccgttgggc cacgaaacga cgctggcgca caaacgcgtc
     9601 gagccagtgg ttccgaagtc caccgagcag ggctggtata tcgacaaatc acgctttgat
     9661 ttccatatcg atccggtgct gaaccagtcg gtcggtggcc cggaaaactt ttacatgtat
     9721 cagctgggcg tgatgggaac ggcagatttg tggctgacgg accatctact gaccaccggc
     9781 agcctgtttg caaatcttgc caacaactac gacaagttta actacaccaa tccaccgcag
     9841 gactcgcact taccgcgcgt tcgtacccat gtgcgcgagt atgtgcagaa cgatgtctat
     9901 gtgaataacc tgcaagccaa ctacttccag catctgggca acggcttcta cggtcaggtc
     9961 tatggaggtt atctcgaaac catgtttggc ggtgcggggg ccgaagtgtt gtatcgtccg
    10021 ctggacagta actgggcgtt tggtctggat gccaactacg ttaaatagcg cgactggcgt
    10081 agtgcaaaag atatgatgaa attcaccgac tacagcgtga aaaccggaca tctgaccgcc
    10141 tactggacgc catctttcgc ccaggacgtg ttggtgaaag ccagcgtcgg gcagtatctg
    10201 gcaggggata aaggcggcac gctggagatc gctaaacgct ttgacagcgg cgtggtggtg
    10261 ggtggctatg ccacgatcac caacgtttcg aaagaggagt acggcgaagg ggacttcacc
    10321 aaaggcgtgt atgtctcggt accgttggat ctcttctcgt ctggcccgac acgcagccgt
    10381 gcggcgattg gctggacgcc gctgacgcgt gacggtggtc agcaacttgg gcgtaagttc
    10441 cagttgtatg acatgaccag cgaccgtagc gtaaatttcc gctaagtcat gggaaaggtg
    10501 ccagttttcg cactcactgg caccctcatt cttaatgacc tgttgagcca tcaggaacgc
    10561 agttcacccc cggtttacga tctggtgtgt aaaggcgaaa acattctgtc ttcctgtccg
    10621 tcgatatcgg ttgcaggtct ggttgcctga gcgactgggc catcattggg ctggagacat
    10681 tgctttgcgg taggtcggct ttatcagcag taccgagcgg accagcataa gcaggaagaa
    10741 cagagactga taacatcaaa gcagcaaaat aaggcttcat ttttaccacc tttatcaggt
    10801 tacgtttcat ttgttccaga ggatcattgt cgatttttcg cgcattgctg gtggctggga
    10861 atcacctgaa tgggtgattt ttgaattacc ggctttggtg cggtttgtct tgccggatgc
    10921 gccgccaggc gcggcttatc cggcctacgg gtaggtatat ccggctttgg tggaggcgcg
    10981 ctccaaatcc aggttgaaca aaacatacac aaaaaatata gatctccgtc acatttttgc
    11041 gttatacagg aagctcgcca ctgtgaagga ggtactgcta tgacgtcact ctctcgtccg
    11101 cgcgtggagt ttatctccac cattttgcag accgtactca atcttggcct gctgtgcctc
    11161 ggcctgattt tggttgtctt cctcggcaaa gaaacggtgc atctggctga tgtgctgttc
    11221 gcgccagaac aaaccagcaa atatgagctg gtagaaggac tggtggttta ctttctctat
    11281 ttcgaattta tcgcgctgat tgtgaagtac tttcagtccg gttttcactt cccgctacgt
    11341 tactttgtct atattgggat caccgccatt gtgcgcttga tcatcgtcga tcataaatcg
    11401 ccactggatg tgttgatcta ctcggctgcg atcctgttac tggtgatcac cctgtggctg
    11461 tgtaattcga aacggctgaa gcgggagtaa aaagacagca cgccgaaatg gcgcggcgtg
    11521 ctggacagga agattacagc gtagcagttt gttgtgtttt cttcgtttcc ggttcccaga
    11581 gcgcttccag ctcctcaagg gttttacctt tggtttccgg gacaaatttc cacataaaca
    11641 gtgctgccag aacgcccata caaccgtaaa tccagtagga gaaaccgttg tggaaatggg
    11701 ccaccagcca ggagtttttg tccatcatcg ggaaggtcca ggagacgaag tagttcgcca
    11761 gccactgggc cgccaccgcg attgccagcg ctttaccacg aatagcattc gggaagattt
    11821 ccgacagcag tacccagcat accggacccc aggacatggc aaaggcggca acatagaaca
    11881 gcatcgacag tagcgccaca atacccgatg cctgagtgta aaacgcggta ccgaggctaa
    11941 acataccgat tgccattccg agtgcgccga taatttgcag tggcttacga ccaaatttat
    12001 ccacagtcat aattgccagt acggtaaagg tgaggttgat aactccgaca ataatggtct
    12061 gcaacagcgc gatatccgtg ctggccccca gcgttttgaa cacttccggc gcgtagtaca
    12121 gcaccacatt gatgccgaca aattgctgga agatggagag cataacgccg attacaatca
    12181 cgcccacgcc aaacatcagc agacgaccac cggttttgcg gccatgatcc agggagtgtt
    12241 taatttcctg tactgcctga gttgcaagcg tgttgcccat aattttgcgc aggatacttt
    12301 ccgcctgttc ttgcttgccg cgtgacatca gccagcgagg actttctggc acggtataca
    12361 gcagcattaa gaacagcagt gcagggatac attccgaggc aaacatataa cgccagccgt
    12421 cagtattcag ccagctggca tcaccggaac gggcaataaa atagtttacg cagtaaacta
    12481 aaagttgccc gaaaataatc gcaaactggt taaatgaaac cagttttccg cgaatatgag
    12541 ctggagccag ttccgcaata tacattggtg aaagcattga ggctaaacca acgccaatac
    12601 cgccaataat acgataaata acaaattccg ggacataacc tgccagataa ataggtactg
    12661 tgttgtccgg gtttatagag gtaaaaccaa gttctggcca ggcagaacct acaccagaaa
    12721 taaaaaacag gacagcagaa atcttaagtg aatcacgacg gccgaagcgg ttactgcaat
    12781 aaccaccgag ggcaccgccg atgatgcaac caatcagagc gctggctacg caaaatccta
    12841 acagggagtt ggcagcggat tcacttaagt tttgtggagc aacaaagacg gtattgagtg
    12901 actcaacagt accggaaata acggcggtgt cgtagccaaa taataaacca cctaatgtag
    12961 cgactaaggt aatcgaaaat atataactgg aattatactg ggtattcatt cagacctgcc
    13021 ttagaccatt ctgatctttt atcagtgcat tgatgttgga attgtcaggc agttaaggca
    13081 attatcattt tttacaatgc gtttacgtaa tgtcttttct gtgatcttaa ttgtgataat
    13141 tatcaaaaat tgatgaaaac aatactattg ccgtgactca gagcacgaaa gagaattatc
    13201 gtaagtggga aaacaaataa cgtaaaaata ataagttcta tgatgggaat attaaccggc
    13261 gaacgattca gattgcagac gaaagaaaaa aaggcgctcc gtggagcgcc gaataatagt
    13321 cacaagttgg gataacgtaa gttgagggtg cagcggcata acattggcag aacaacatct
    13381 ttaacctttc acaccacctg ccgtcaggcc gttgaccagc cagcgttgag ccagcaagaa
    13441 gacgatggtg atcggtaatg cagacatcac ggcagcggcg gcaaagtcac cccacaggta
    13501 gttttgcggg ttgaggtatt gctgcatccc cacggccagg gtgtagctgt ttacgtcacg
    13561 cagtaacagc gacgcgaccg gaacttcagt aatggcagcg ataaacgaca ggatgaatac
    13621 caccgccaga atcggtactg acagcggcaa caggacaagg cggaaggcct gccacggtgt
    13681 cgcaccatcc agcgcagccg cttcttccag cgaactgtcg atggtttcga aatagccttt
    13741 gatggtccag acatgcagcg caatcccacc cagatacgcg aaaattacgc cgccgtgagt
    13801 attcaggcca atgaatggaa tgtactcacc cagacgatca aacaacgcat acaacgcgac
    13861 cagtgaaagt actgccggga acatctggaa aatcagcatt cctttcagca gcgtcgcttt
    13921 gcctggaaag cgcatacggg cgaaagcgta ggcgcaggtg gtggagagcg ccacaatgcc
    13981 aatcgcggaa atcccagcga cctttaccga gttccacagc cacagcagta ccgggaatgg
    14041 cggtggcgta atgcgaccat cagcctgttc aacgctaaaa cctaacgcca gtttccagtg
    14101 atcccaggag atttgctccg ggatcaggct accggtcgcg aagtttccct gacgcagcga
    14161 gatagcgacg accatcagca gcgggaacat aatcgccgcg ataaaaagta gcagtagcag
    14221 gtgagtaata aacaaacgtg ctttttgcga tttcggttgg accattgcca tttttgttat
    14281 ctcccttaat caaacttcat tcgcgtggct ttcaggttca ctatcgccag cgcacccacc
    14341 agcaggaaga tcagcgtggc aattgctgcc gccagaccga agtcctgacc cccgccgcct
    14401 tcaaaagcga tacggtaggt gtagttaaca agcaggtcgg tataaccggc tggcgtggtc
    14461 gtgccaagac gatccgggcc gccgttggtt aacagctgaa tcagcacgaa gttgttaaag
    14521 ttaaaggcga agctggcgat catcagcggc gtcagcggtt taatcagcag cggcagcgta
    14581 atcttaaaga agttctggaa cggacctgcg ccatccattg ctgaggcttc atacaaatcg
    14641 tccggaatcg ctttcagcaa gcccatgcag aggatcatca tgtacggata acccagccag
    14701 gtattgacga taatcagcat cgtgcgagcg gtggtcggat cgctgaacca ggcgggcttc
    14761 acgccaaaca gcgcgctcaa catcatgttg atttcaccga agctctggtt aaacaatcct
    14821 ttgaaaatca agattgaaat gaacgatggc accgcgtagg gcagaatcag caggacgcga
    14881 tagaccgctt tgccgcgcaa cgcttcccac tgcaccagac atgccagaac catgccgacc
    14941 gccaccgtta aaaagacagt gatcagcgag aacaccacgg tccagacgaa aatggcgagg
    15001 aacggtttct gaatgccttc gtcggtaaag acgcgggtaa agtttttcca gccggtggtc
    15061 acggtgtaac cggggcttag cttttcatcc ccccagttgc cgtcggcggt aatggactgg
    15121 taaaagccaa tttggttatt cggacgatat ttcacgccac tctgattatt cgtcaacgtg
    15181 ccgtcaccgt cgagtgtgta gagcggctgc gtgccagaaa actggcgcag ggagctcatc
    15241 atcactttgt tgccatccgg cagaatggcg gtaatgtcac tcagcgcctg acgattctgg
    15301 gtaatcacgc gcagattcgc gcgttcgcct tcgggctggg cggtcgtttc tttcagttgc
    15361 agtttttgct cgccgccaaa tttaaacgcg tcggagaggt aatttttgcc ggtttcgccg
    15421 tcgctgagcg ccagttgcca ctcatcgccc gccgggtaaa gaccaaagtt ataggttttg
    15481 cctgcttgcc aggagcgatc taacaacact tcctgcgcac gttcaaaagt cagctggtta
    15541 gtgctgctgt agttggtgaa ggcaatggcg atggtgcaga ccagagggaa gaggacgaat
    15601 aatcccattc cagccattcc cgggtaaaca tagcgccagg cgtaggcttt acgattggcg
    15661 aaaatataca gccccgctga actcaatatc agcgtggtaa tggcgaacag gtattcccct
    15721 tgtgcgtaca ttaaaacaac aaggtaaccc accagcaggc cgagcagacc tagcactgac
    15781 catttcagcg cgtcgctttg ccaccaatgt ttctttttaa tgacatccat ggggttcttc
    15841 ctcattccag gacggataag gctttcacgc cttatccgac aacaactgcc tgatgcgaca
    15901 ctgatgcgtc ttatcaggcc tacatgcgtt tcagttttgt aggccggaca aggcgttcac
    15961 gccgcatccg gcatttcaca gcattacttg gtgatacgag tctgcgcgtc tttcagggct
    16021 tcatcgacag tctgacgacc gctggcggcg ttgatcaccg cagtacgtac ggcataccag
    16081 aaagcggaca tctgcgggat gttcggcatg atttcacctt tctgggcgtt ttccatggtg
    16141 gcggcaatac gtggatcttt cgccaactct tcctcgtaag acttcagcgc tacggcaccc
    16201 agcggtttgt ctttattaac cgcttccaga ccttcatcag tcagcagata gttttcgagg
    16261 aactctttcg ccagctcttt gttcggactg gcggcgttaa taccggcgct cagcacgcca
    16321 acgaacggtt tagatggttg acccttgaag gtcggcagta ccgttacacc ataattcact
    16381 ttgctggtgt cgatgttgga ccatgcccac gggccgttga tggtcatcgc tgtttcgcct
    16441 ttattaaagg cagcttctgc gatggagtaa tcggtgtctg cattcatgtg tttgttttta
    16501 atcaggtcaa ccaggaaggt cagacccgct ttcgcgccag cgttatccac gcccacgtct
    16561 ttaatgtcgt acttgccgtt ttcatactta aacgcataac ccccgtcagc agcaatcagc
    16621 ggccaggtga agtacggttc ttgcaggttg aacatcagcg cgctcttacc tttcgctttc
    16681 agttctttat ccagcgccgg gatctcttcc caggtttttg gcgggttcgg cagcagatct
    16741 ttgttataaa tcagcgataa cgcttcaaca gcgatcgggt aagcaatcag cttgccgttg
    16801 taacgtacgg catcccatgt aaacggatac agcttgtcct ggaacgcttt gtccggggtg
    16861 atttcagcca acaggccaga ttgagcgtag ccaccaaagc ggtcgtgcgc ccagaagata
    16921 atgtcagggc catcgccagt tgccgcaacc tgagggaatt tctcttccag tttatccgga
    16981 tgctcaacgg tgactttaat tccggtatct ttctcgaatt tcttaccgac ttcagcgaga
    17041 ccgttatagc ctttatcgcc gttaatccag attaccagtt taccttcttc gattttggcg
    17101 agagccgagg cggaaaacat catcgtcgtt aatgcggata atgcgaggat gcgtgcacct
    17161 gtttttattt tcataatcta tggtccttgt tggtgaagtg ctcgtgaaaa cacctaaacg
    17221 gactctagtt tctttatacg gcaacctctt tccatcctcc ttgcccctac gccccaccgt
    17281 tgctttgtgt gatctctgtt acagaattgg cggtaatgtg gggatgcgca cataaaatcg
    17341 ccacgatttt tgcaagcaac atcacgaaat tccttacatg acctcggttt agttcacaga
    17401 agccgcgttc tcatcctccc gcctcctccc ccataaaaaa gccagggggt ggaggattta
    17461 agccatctcc tgatgacgca tagtcagccc atcatgaatg ttgctgtcga tgacaggttg
    17521 ttacaaaggg agaagggcat ggcgagcgta cagctgcaaa atgtaacgaa agcctggggc
    17581 gaggtcgtgg tatcgaaaga tatcaatctc gatatccatg aaggtgaatt cgtggtgttt
    17641 gtcggaccgt ctggctgcgg taaatcgact ttactgcgca tgattgccgg gcttgagacg
    17701 atcaccagcg gcgacctgtt catcggtgag aaacggatga atgacactcc gccagcagaa
    17761 cgcggcgttg gtatggtgtt tcagtcttac gcgctctatc cccacctgtc agtagcagaa
    17821 aacatgtcat ttggcctgaa actggctggc gcaaaaaaag aggtgattaa ccaacgcgtc
    17881 aaccaggtgg cggaagtgct gcaactggcg catttgctgg atcgcaaacc gaaagcgctc
    17941 tccggtggtc agcgtcagcg cgtggcgatt ggccgtacgc tggtggccga gccaagcgta
    18001 tttttgctcg atgaaccgct ctccaacctc gatgctgcac tgcgtgtgca aatgcgtatc
    18061 gaaatctccc gtctgcataa acgcctgggc cgcacaatga tttacgtcac ccacgatcag
    18121 gtcgaagcga tgacgctggc cgacaaaatc gtggtgctgg acgccggtcg cgtggcacag
    18181 gttgggaaac cgctggaact gtaccactat ccggcagacc gttttgtcgc cggatttatc
    18241 ggttcgccaa agatgaactt cctgccggta aaagtgaccg ccaccgcaat cgatcaagtg
    18301 caggtggagc tgccgatgcc aaatcgtcaa caagtctggc tgcctgttga aagccgtgat
    18361 gtccaggttg gagccaatat gtcgctgggt attcgcccgg aacatctact gccgagtgat
    18421 atcgctgacg tcatccttga gggtgaagtt caggtcgtcg agcaactcgg caacgaaacc
    18481 caaatccata tccagatccc ttccattcgt caaaacctgg tgtaccgcca gaacgacgtg
    18541 gtgttggtag aagaaggtgc cacattcgct atcggcctgc cgccagagcg ttgccatctg
    18601 ttccgtgagg atggcactgc atgtcgtcga ctgcataagg agccgggcgt ttaagcaccc
    18661 cacaaaacac acaaagcctg tcacaggtga tgtgaaaaaa gaaaagcaat gactcaggag
    18721 atagaatgat gattactctg cgcaaacttc ctctggcggt tgccgtcgca gcgggcgtaa
    18781 tgtctgctca ggcaatggct gttgatttcc acggctatgc acgttccggt attggctgga
    18841 caggtagcgg cggtgaacaa cagtgtttcc agactaccgg tgctcaaagt aaataccgtc
    18901 ttggcaacga atgtgaaact tatgctgaat taaaattggg tcaggaagtg tggaaagagg
    18961 gcgataagag cttctatttc gacactaacg tggcctattc cgtcgcgcaa cagaatgact
    19021 gggaagctac cgatccggcc ttccgtgaag caaacgtgca gggaaaaaac ctgatcgaat
    19081 ggctgccagg ttccaccatc tgggcaggta agcgcttcta ccaacgtcat gacgttcata
    19141 tgatcgactt ctactactgg gatatttctg gtcctggtgc cggtctggaa aacatcgatg
    19201 ttggcttcgg taaactctct ctggcagcaa cccgctcctc tgaagctggt ggttcttcct
    19261 cttttgccag caacaatatt tatgactata ccaacgaaac cgcgaacgac gttttcgatg
    19321 tgcgtttagc gcagatggaa atcaacccgg gcggcacatt agaactgggt gtcgactacg
    19381 gtcgtgccaa cctgcgtgat aactatcgtc tggttgatgg cgcatcgaaa gacggctggt
    19441 tattcactgc tgaacatact cagagtgtcc tgaagggctt taacaagttt gttgttcagt
    19501 acgctactga ctcgatgacc tcgcagggta aaggtctgtc gcagggttct ggcgtcgcgt
    19561 ttgataacga aaaatttgcc tacaatatca acaacaacgg tcacatgctg cgtatcctcg
    19621 accacggtgc gatctccatg ggcgacaact gggacatgat gtacgtgggt atgtaccagg
    19681 atatcaactg ggataacgac aacggcacca agtggtggac cgttggtatt cgcccgatgt
    19741 acaagtggac gccaatcatg agcaccgtga tggaaatcgg ctacgacaac gtcgaatccc
    19801 agcgcaccgg cgacaagaac aatcagtaca aaattaccct cgcacaacaa tggcaggctg
    19861 gcgacagcat ctggtcacgc ccggctattc gtgtcttcgc aacctacgcc aagtgggatg
    19921 agaaatgggg ttacgactac aacggcgata gcaaggttaa cccgaactac ggcaaagccg
    19981 ttcctgctga tttcaacggc ggcagcttcg gtcgtggcga cagcgacgag tggaccttcg
    20041 gtgcccagat ggaaatctgg tggtaatagc aaaacctggg ccggataagg cgtttacgcc
    20101 gcatccggca taaaaacagg ttgtcattat ctgaaagggg cgaaagcccc tctgattatc
    20161 gggtttagcg cgctattgcc tggctaccgc tgagctccag attttgaggt gaaaacaatg
    20221 aaaatgaata aaagtctcat cgccctctgt ttatcagcag ggttactggc aagcgcgcct
    20281 ggaattagcc ttgccgatgt taactacgta ccgcaaaaca ccagcgacgc gccagccatt
    20341 ccatctgccg cgctgcaaca actcacctgg acaccggtcg atcaatctaa aacccagacc
    20401 acccaactgg cgaccggcgg ccaacaactg aacgttcccg gcatcagtgg tccggttgct
    20461 gcgtacagcg tcccggcaaa cattggcgaa ctgaccctga cgctgaccag cgaagtgaac
    20521 aaacaaacca gcgtttttgc gccgaacgtg ctgattcttg atcagaacat gaccccatca
    20581 gccttcttcc ccagcagtta tttcacctac caggaaccag gcgtgatgag tgcagatcgg
    20641 ctggaaggcg ttatgcgcct gacaccgtcg ttggggcagc aaaaacttta tgttctggtc
    20701 tttaccacgg aaaaagatct ccagcagacg acccaactgc tcgacccggc taaagcctat
    20761 gccaaaggcg tcggtaactc gatcccggat attcccgatc cggttgctcg tcataccagc
    20821 gatggcttac tgaaactgaa agtcaaaacg aactccagct ccagcgtgtt ggtaggacca
    20881 ctatttggtt cttccgctcc agctccggtt acggtaggca acacggcggc accagctgtg
    20941 gctgcacctg ctccggtgcc ggtgaagaaa agcgagccga tgctcaacga cacggaaagt
    21001 tattttaata ccgcgatcaa aaacgctgtc gcgaaaggtg atgttgataa ggcgttaaaa
    21061 ctgcttgatg aagctgaacg cctgggatcg acatctgccc gttccacctt tatcagcagt
    21121 gtaaaaggca aggggtaatt acgccccaca gtgctgattt tgcaacaact ggtgcgtctc
    21181 ctggcgcacc tttttttatg cttccttcct gggatatgag cgatttttta tagtacttca
    21241 cttcttcttc actaagaatg tccattattt caatgcatta tcagagattc ttttcctttc
    21301 gccggtagtg tctggacgct caggctactt ttccaggtta ttttatttct gtattacaga
    21361 ggttttatga taggtcatat cacaaattct ggcggcaata actctttgat gaaacatgat
    21421 gtggtgcaag gaaataatat agtagatctt gatttactac gtaatttcaa tggggtgaca
    21481 ggtttaaaca gagataactt tatttatatc agtgatatat ttttaaatat aaaacaacgg
    21541 aacgaaaaaa atcatgcaat aaatatgtta cgtgaagttt caattagtaa tgatactata
    21601 agtgtaaaat tctacagaaa tgaaaaaata gaatgcgctt gtaattttct gatggataaa
    21661 gatgcgcagg ggtatatcga cctgtctgat tttgatttaa caaattgtca ttttaaaggt
    21721 gacgttattt ctaaggtg
//
LOCUS       DADLSA010000054        21686 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.054, whole genome shotgun sequence.
ACCESSION   DADLSA010000054 DADLSA010000000
VERSION     DADLSA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.054"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(15..1010)
                     /gene="yjfF"
                     /locus_tag="JN691_002093"
     CDS             complement(15..1010)
                     /gene="yjfF"
                     /locus_tag="JN691_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HAY7243542.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(997..2019)
                     /locus_tag="JN691_002094"
     CDS             complement(997..2019)
                     /locus_tag="JN691_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7243543.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2033..3535)
                     /locus_tag="JN691_002095"
     CDS             complement(2033..3535)
                     /locus_tag="JN691_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7243544.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQFGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3675..4631)
                     /gene="ytfQ"
                     /locus_tag="JN691_002096"
     CDS             complement(3675..4631)
                     /gene="ytfQ"
                     /locus_tag="JN691_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HAY7243545.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            4941..5471
                     /gene="ppa"
                     /locus_tag="JN691_002097"
     CDS             4941..5471
                     /gene="ppa"
                     /locus_tag="JN691_002097"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAY7243546.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5851..6192)
                     /locus_tag="JN691_002098"
     CDS             complement(5851..6192)
                     /locus_tag="JN691_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY7243547.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="JN691_002099"
     CDS             complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="JN691_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAY7243548.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="JN691_002100"
     CDS             complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="JN691_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAY7243549.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11910..12539
                     /gene="msrA"
                     /locus_tag="JN691_002101"
     CDS             11910..12539
                     /gene="msrA"
                     /locus_tag="JN691_002101"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY7243550.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGVCLPPEA"
     gene            12862..14205
                     /locus_tag="JN691_002102"
     CDS             12862..14205
                     /locus_tag="JN691_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY7243551.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14267..14473)
                     /locus_tag="JN691_002103"
     CDS             complement(14267..14473)
                     /locus_tag="JN691_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAY7243552.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14798..15352
                     /locus_tag="JN691_002104"
     CDS             14798..15352
                     /locus_tag="JN691_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY7243553.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15415..16353)
                     /locus_tag="JN691_002105"
     CDS             complement(15415..16353)
                     /locus_tag="JN691_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243554.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16565..17305)
                     /gene="cysQ"
                     /locus_tag="JN691_002106"
     CDS             complement(16565..17305)
                     /gene="cysQ"
                     /locus_tag="JN691_002106"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAY7243555.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17495..19433
                     /gene="cpdB"
                     /locus_tag="JN691_002107"
                     /pseudo
     CDS             17495..19433
                     /gene="cpdB"
                     /locus_tag="JN691_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
     gene            complement(19559..19939)
                     /locus_tag="JN691_002108"
     CDS             complement(19559..19939)
                     /locus_tag="JN691_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY7243556.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYSVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20028..20888
                     /gene="qorB"
                     /locus_tag="JN691_002109"
     CDS             20028..20888
                     /gene="qorB"
                     /locus_tag="JN691_002109"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY7243557.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            20997..>21686
                     /locus_tag="JN691_002110"
     CDS             20997..>21686
                     /locus_tag="JN691_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7243558.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDCARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRP"
BASE COUNT         5224 a         5812 c         5340 g         5310 t
ORIGIN      
        1 acggagaggc atttttatcg ctgcgcaatg ttgactcttg tcactggcga actctgacga
       61 ttctcccaca gcaccgttaa cccacgctgt aatgctataa aaataaacaa caaaataccg
      121 atggcgattt tcgtccacca ggaactcagc gtgccgtcaa agttgatgta agtctggatc
      181 agtccctgaa ttgccacgcc aaaaagcgtc ccaagcaccg tcccgacgcc accacttaac
      241 aacgtaccgc caatcaccac cgacgcaatg gcgtccagtt ccacacctac gcccgccagt
      301 gcatatccgg cttgggtata aatcgagaag acaatccccg ccagtgttgc cagtccggtg
      361 gagagcatat aaatgcgaat agtggtgctg cgagtagaaa tccccatcag gttcgccgac
      421 gtcgcgttgc cgccaatagc gtacacctga ttgccaaaac gggtacgatg cgcaaggaaa
      481 ataccgataa ccaccaccgc cagcatcaac agccccatcg cactaaggcg accgccgccg
      541 gggattttcc acgcaaggct tgagagcgtg tcataaatgg gatggtttat cggaatcgac
      601 tcttccgaaa cgagatagct gacgccgcgc aaaaagaaca tccccgcaag cgtaatgata
      661 aatgccggga tcttcagggc gtcgatcaga agccccataa atgcgccgaa ggcacagccc
      721 atcaccagca ctagcggaaa cgccagcagc ggcgagaggc cgaaatcgcc aaccactttt
      781 gccagaaaca cgccagtaaa ggcgatcacc gaaccgacgg agagatcgat accgccagag
      841 aggatcacaa aggtcatgcc aacggcgatt atcccaagaa aggcgttatc ggtcaggata
      901 ttgcagatca cccttgtgga agcaaaaccg ggaaactggg tcaggcagta gagatagccc
      961 agcacaaaga cgccgatggt gatcatcagc ggcaagttac gttttatcat ggctacgtac
     1021 tcctttaatc agactgataa agcgttgcga ctgaacaatc agcacacaaa gcaccaccac
     1081 cgctttcacc acctggttca tctccggcgg aaagcccgaa agcaaaattc cggtgttcat
     1141 cccctgaata atcagcgccc ccactaccga gagcagcagg ttaaagcgcc cgcccatcag
     1201 cgatccgccg ccaatcacca ccgcgagaat ggcgtccagc tccagccata atccggcgtt
     1261 gttggcatcg gcaccgcgaa tatccgccgc cacgataatg cccgcaatcg ccgcacatag
     1321 cccgctcaac acgtaggtga gcatgacgat gattcgcgtg tttaccccgg cattttttgc
     1381 cgcccgaatg ttgataccaa cggcttcgat aaacatcccc agcgccgttt tgcgggtcaa
     1441 cagccagaac aggagaagcg tcagcaccgc gataatgacc ggcgttggca ggaacaacag
     1501 cgatccgctg ccaaaccatg agagatccgg cgagttaaac gtgacgatct gcccggcggt
     1561 gatcagttgc gccacgccgc gcccggcgac catcaggatc agggtggcga caaacggctg
     1621 aattttgagg atcgctacca gtatgccgtt ccacaatccc gccaggatgc cggtgcccag
     1681 ggcgcttaac aaaacaatcg gcaggctgaa tcccgcgacc gtcatcgcag ccgttgtggc
     1741 tccggcgata gccatcaccg cccccacaga gagatcgata ccaccggtgg cgatcaccag
     1801 cgtcatacca atcgccagta acgcaacggg agctgcacgg ttaagaatgt cgatagggct
     1861 accgaacaaa cgcccatcct gaagcaccac ctgccagaaa tgcggggcta ccaggctatc
     1921 gaccagcagc accagcaaca gtgccgccag ctgcggcatc cccgtcggcc agtgaaaacg
     1981 cctttttggc ggcgtagtgt cagggagaga ttgaggcatc acactgttct ccttacgccg
     2041 caatggcgtt catgatcgcc ggaacggaaa gctgtgccag cgggatctcc gccacctgtt
     2101 tgcgatcgcg catgatgatc acccggtcgg catagcccac cagctcttcc agttcggagg
     2161 agatcaccag cagcgccaga ccgtcagcac acagcgtttc aatcaggcgg atgatctcgg
     2221 cgtgggcacc aacatcaatg ccacgggttg gctcatcaag gatcagaaat tgcggtcggg
     2281 tcagtagcca acgtgaaagc agcacttttt gctgattgcc accggagaga aattcaatcg
     2341 gttgttcagt tgaaggtgtg cgaatgccaa actggcggat aaagcgttcg gcaatctctt
     2401 gctgctcttt gcgggaaatg ggtcgtagcc agccgcgctg ggcctggaga gcgaggatga
     2461 tattttcccg caccgaggcg gcggcgatga tgccatcggt tttcctgtct tccgggcaga
     2521 atccgatgcc cagtactgaa gcctgatgtg gcgatcgcag ggtttgcggt ttgcctttga
     2581 tcaacgccgt gccgctgtca gcaggtttga taccgaagat cacttcggcg gtttcggtac
     2641 gtccggatcc cagcaagcca gccagaccga cgatctcgcc ggggcgcact tcgagatcaa
     2701 acggtgctat cgttcctttt ttgccgtaat ttttgaacgc ggcaacgggt ttgtcgctca
     2761 acaatgttcg cccggcacgc tgtagcgcgt gggtatccag ctcgcgcccc agcatcattt
     2821 ttaccagttc gatctgcggt agctcgcgcg tttcccgaca gcctacgaaa ctgccgttgc
     2881 gtaagacggt gatccgatcg ctgacctgat agacctgatc gagaaagtga gtgacaaaga
     2941 tcaggctgac gccgcgatcg cgcaactgac gcatcaggcc aaataatagc tccacttcct
     3001 gggtgtcgag gctggcggtg ggttcatcga ggatcagcac tttggcggag agatcgatag
     3061 cccggcaaat ggcgacgatt tgctgcatcg ccaccgaaaa acggttaagc ggttcgcgca
     3121 cgtcgaggga gaaaccgtaa gatgccatca gttcggtggc gcgcttttcc atctctttac
     3181 ggcgtagaag gccgaaacgt ttgggttcgc gacctatgaa tagattatca gcaaccgaca
     3241 tattgggtag caggttgact tcctgataga cggtgccgat accgagttgt tgtgcatggg
     3301 cggtattttt cggtgagata gcctggcctt ccagccagat ggtgccgcga tcggcgtggt
     3361 atacaccagt taatgctttg attagcgttg attttcccgc cccgttttca ccaagcagcg
     3421 ccatgatttc gccacggcgc aggctgaaat caacgttgtc taacgctttg acgccgggga
     3481 aaaatttact taatccttcg gtgcggagga tctcctggtg ttggtcggtc gtcatggttt
     3541 cccccggtgt aaagcgtgct cccagtaggc atgataagac gcggtaagcg tcgcatcagg
     3601 cttcagtgca ccatcgccgg atgcggcgcg agcgccttat ccggcctacg aatcggcatc
     3661 aggcatagca accctcaata ccccatattt ttcttcttct ctaactcttc ttttgcggta
     3721 tcaggcaggt aaagtgtgga tttggttaac gtcagctttt caggcatggt gccgtctttt
     3781 ttgtatttct ccagcgcatc gaaagcgggt cccgccatgt tcggcgtcag ttcaacactg
     3841 gcgttcgctt cgccatccat catcgctttg tagatgtccg gtacaccgtc aatggaaccc
     3901 gtgaggatat ctttgcccgg tttcaggccc gcttctttaa ttgcctgaat tgcaccaatc
     3961 accatgtcgt cgttatgggc gtaaaccatg cagatgtttt tgccgttgtt ttccgctttg
     4021 ataaagctct ccatgacttc tttgccttta ctgcgggtga agtcacctga ctgcgagcgg
     4081 ataattttga tatttggcgc attcttaatg gcttcggcaa agcctttctt acggtcaatg
     4141 gcgacgctgg ccccaacggt gccctgcagc tccaccacgt tgcatggttt gccattcact
     4201 tcttttacca gccagtcacc gatcaactta ccttcgagga tgttgtcggc agtaacggtg
     4261 gtcatataga gagatttgtc tttcacatcg atggaacggt cgagcaagaa gaccgggatt
     4321 tcggcatctt tcgcctcttt taataccggt tcccaacccg tcgcaaccac tggagcgata
     4381 aagatcgcat ccaccccttg tgcaacgaag gaacgtaccg ctttaatctg gttttcctgc
     4441 ttttgctgac catcggcaat tttcaacgta attccgcgtt tttcggcctc acttttcgcc
     4501 acattggttt ctgcggcgcg ccagccggat tccgatccga cctgcgaaaa tcctacggtt
     4561 aatggagcgg ccaacgccat agacgacatg gctgccgaga ctgcagagac tacaagtaag
     4621 cgtttccaca tagggttgtc ctcgtcgggg taattattat tggtgaaaag atgttcgcga
     4681 aaaaactata gacaattcgt tatgtaacgg attgcgttac atcacacttc aaaaaagtaa
     4741 ataaaacgtt aatcacaagt ttgtaatcgc tttcatctca ctatgaaaaa tgcggctacg
     4801 gttatggatt ttcctgctct gtataccgtc ttaaaactgg cgaaaaagga aaatgaagac
     4861 gaaaacaagc aaagacattc ggcgcgagtt ggctataata ctcggcactt gtttgccaca
     4921 tatttttaaa ggaaacagac atgagcttac tcaacgtccc tgcgggtaaa gatctgccgg
     4981 aagacatcta cgttgttatt gagatcccgg ctaacgcaga tccgatcaaa tacgaaatcg
     5041 acaaagagag cggcgcactg ttcgttgacc gcttcatgtc caccgcgatg ttctatccgt
     5101 gcaactacgg ttacatcaac cacaccctgt ctctggacgg tgacccggtt gacgtactgg
     5161 tcccgactcc gtacccgctg cagcctggtt ctgtgatccg ttgccgtccg gttggcgttc
     5221 tgaaaatgac cgacgaagcc ggtgaagatg cgaaactggt tgcggttccg cacagcaagc
     5281 tgagcaaaga atacgatcac attaaagacg taaacgatct gcctgaactg ctgaaagcgc
     5341 aaatcgctca cttcttcgag cactacaaag acctcgaaaa aggcaagtgg gtgaaagttg
     5401 aaggttggga aaacgcagaa gccgctaaag ctgaaatcgt tgcttccttc gagcgcgcaa
     5461 agaataaata agttcttcta gcgcaataac cctgaacgcc gggcttcggt tagtaagggt
     5521 ttttttatgc ccgcgataaa taaactctct attccaccat cattattctc agcggttgca
     5581 aggcttgaac ggtaagaaca agcaaacccg accaccattt tgctgttcat agccacttgc
     5641 tggaagttag ccgacctcac tcatactcac cgttcaaagc gtgcccccgt tttacccctg
     5701 cacatttgtg aacgccagca gagtttttta tgcgattgca gatatggaaa gcggctcgga
     5761 gagaaaagca aaagatggaa aagttacaaa aaaaaatcgc gagatacatc gcataaaaca
     5821 acgccacccg aaggtggcgt gcttttatta ttacttatcc ctgtctaacc agtcgccgct
     5881 ttcaattagc tttaatccat cgacgggtcg ttgataaacg tacatccatg cactcccata
     5941 cggcgtctga atcaactggc gcgcgtattc accgcccctg gtgcgcaagg catcaagttc
     6001 ggccagcgtg gcgttgtcaa tacgataaac ttcaccgtgt accgttccgt tccccggaac
     6061 tgcgcctgga tagtggccca ggctatacaa ctggtagtta tcgatactga aatcgcccag
     6121 taactgggca ttggtcatcc agtgactgtt gccttgtttg tggcgtaaac tgccgtagac
     6181 aaatattcgc attgctaaaa ctcgaactga tagagcaaat ccagtgcctg gtctacacca
     6241 gacacggctt ccagatatag cttaggcatc aggcgataac gtaacgtgag tgttgctata
     6301 gagtcaaata tacccacgcc gtatttcact tgcagacctg gcaatacata gccgctgacc
     6361 actacctggg aggagtcgcc tactcccagg gtgtcgagcg ctaaattgct tacgccaaac
     6421 gtctcgccga ttttacccac aatctggcca ctttgcgcaa cccccagacc aatcagcatc
     6481 gaggtcattg ccgcactgtc gctctgatcg ctctccagcc cttgtccacg tagcaaataa
     6541 gacaatgcag cttgttgcga catcgccggg tcagagaaga tctccgcttt cggttcgtcc
     6601 gccagaccag tgacgcgaac tccggcgatt acatcgtctt ctgtagcatc cgggttacga
     6661 atagcttcaa tattaagata cggttgatcc ggcggaccag agaacagtaa ctcaccctta
     6721 cgcacaatca gatcctgacc ataggcatgg aagcgccctt cagggatgtt gatctgcccg
     6781 ttcaggccca gcccttgttt gtcctgtacg acgttaagat cgcccgtcag ccgcgctttc
     6841 aggccaaagg cgtcaatgcg cacattgttg ccaacgtgga caatcaggtt actgttaatc
     6901 ggaatcgacg ccgttttcgg ctcttccggt tgcaggttat cgttaagcat caccacatcg
     6961 ctggagacgc ctactgcgct ttccggcaga tcgtgcacca cgatgcgcgc ccacgggaca
     7021 tccacgcgac catcgagggt aaacaggttt ggtgtagcct cgaatacaac atctggcgat
     7081 acatccattc gtaccatcgg cggcacggtg atccgcactt tactgccttt cgccgttact
     7141 cgtgcccgcc agttttcaat ttggctccag tcggcatcac cgttcaggta gatttcaccc
     7201 tgctgggtcc gtactgtacc ggcaagcgtc gagcgcatac cgttaaagtt gaccgcaagc
     7261 tggctcggct gcatatcaaa cggcataaag ttgccgtcga tatccacacc cgtaacctga
     7321 agctgaccaa acaactgcgg gctttgcaca tcaccaccca gacgcaagtt ggcactcacc
     7381 atccccgctg ctttttcccc acgggtaaag atggggttta tcatcgcaag gttgaagtta
     7441 cggatattga cgttgccacc aagattacgg cggccttgcg gatcggtcac ctgcacctgt
     7501 ccatcaaact ggccgttatt ggtcaggcgg atggtccagc ccaattcggc acggttgtta
     7561 cgcaattccg ccgtcagatt cagtgtctga aacgccaccg gcagcgccgc atcgttgacg
     7621 gtttgcgtta cctgcacgtt acgccccgaa agggtgatac tgccctgcgg cagcccctct
     7681 ttcgtggtgt cccaggcaac atccgcttta cccgtgaaga taccgctggc ctgagtggtt
     7741 tctggcataa acggtttcag catggcgagg tcgaagcggt tgagattcac caccgcacgc
     7801 ccttcggccc ccgcatcgat agtttgcggc acgcacagtt ccgcattcgg gttaagccag
     7861 cagtgtggcc cgatgctgat tttttgctcc ttattgcggt aatccagcgc aatatcgcgg
     7921 gtcagcgacc acgggccgac cggcgtctgg aagcgggtat tgctaagagt tcccttccag
     7981 cgttcttctt tgcgatcaaa acttcctgcc agattaagct gcccggagac aggctcgccc
     8041 tgaatccgca actgtagctc gtgctgcttt tcgctgcctt tggcattcag ggtgacgagg
     8101 ttgatattta catccggctg cgaaatttgc tcaacgcgta cgtcgagttt cccggcgatc
     8161 tgatcggtgg atttaatgtc gccttccacg cgaacctgcg ccacggaaag ttcctgccag
     8221 cgcaggccgc gcgcggtgat atctgccagt agttgtggcg cttccaccgt gccgcgtact
     8281 ttcaccagcc ctttcgctgt accgccaagc cccggcagcg cgttatccag ccccggcgcg
     8341 ttgatggtgg catcaagatt gagatctttt acccccagct cgcctttcac ttcggcactg
     8401 tttggcccga gttccagatg aagcccaggg atcatccact gcatataact gttgcctttc
     8461 agcgtgccgt caacgttcac tttgttctgt ttgacgttac cggtcagctt cagttctggc
     8521 acctccatct gccaggtgcc gccgtacagg ctaccgcggg ttttaatcaa gccattgagt
     8581 ttcgacggcc actccgggaa ctctttggcg gtgttaatgc cattaagcgt tagctcaccg
     8641 cgccaactaa tggcctgctg ccagtcgagc aacgccttga gttcagtttt cccttccagc
     8701 gccgcgacgg tgagtttgtc gagattgacc tgctgttcat tacctttggc atcaagggta
     8761 atggtggctg gcgggatctc ctgtcccttc actgccgtac gcatagagag cgtgtaatcg
     8821 gtcattttgc cggtaagttt cagtttcaga tcatccgcct gatactgctt ctcaccagtg
     8881 aacggccagt aaagctgttt gctgttcact tccacgttga gtggcaatcc ggcttccgcc
     8941 agtcgcgtct gggcgcgtaa atccatatcc accggaccgg aaaggttaac gcctatctcc
     9001 agctgttcgc gcagcgcgcc gcccactttc agcttcacct tttcaccttt caacggctcc
     9061 acgttcagtg tgctgttgag ggtgatatcc accggccagt tgtctgacag ctgcgccgtg
     9121 ccgctggcgt tgacgatccc ctggttggaa tcgatatcca gggcgtccag tttagtattg
     9181 ccgtcaatgc tgctcacttt cagcagcatg gtgcgcacgg tgatgtccgt gtcgcccgtc
     9241 acgcgcagct gctcgccctt aaactcttca atgttcaggt taagcggcaa atgcacgtcg
     9301 gtcatttccg gcaataccgg gcgagaaaag agatctttca gcgtttcgcc gagcggcttt
     9361 tcctccggct gcggattttc aattttcggt tcgaccactt cttcctgcgc cacttccacc
     9421 actttcggca gagcaatcag caggcctttc agcgacgtcg gtttcagggt cagggttttc
     9481 tcctgccagt tcaggccgga ggtgaagtcc atcaccgaca ccgtggtgtc atcaatcttg
     9541 atgttgacgt tgtccagcgc tacccgtgtc agggtgatgg gatacggcgt ggagagatcc
     9601 agcggaccgc tatcttcctc ttcttcaacc tgttcagaag gaggcatttt tttactgtcg
     9661 atgttgacct gaatgtcttt cagcgccagg tcattaatac aaacgctact gttccacagg
     9721 cactcaagac cgaccgccag atgcagatta cccgctttta ccgccacgcc tggctgctca
     9781 taacgaacgt cagacaaggt gagatcgcgc cagccgccgg tgaccttgcc aatatccagt
     9841 cctggcaccc agcgatccgc cgctttaaat acaagatgca ggccgctggt ggtgcccacc
     9901 agaaacgcca ccgatcccag caacagtaag ataacgataa ccacgccgag gctgattttt
     9961 ttccataaac tcataattct ggccccagac cgatgtaaaa ctgtaacccg tgttcgtctt
    10021 tatccgcgac cggtacggca aaatcgagtt tgattggccc gaccggcgat tcccagcgta
    10081 cgccgacccc ggtaccggtt ttaaagtcgc tgcggcgaat atcgcttacc gcttcgccac
    10141 tatcgacaaa caccgcgccc caccattttc cggtcacgtt gtactggtat tccagcgatc
    10201 cggttatcaa cttcgaggcc cctttcaggt caccgttggc gtatttcggg gcgatagatt
    10261 tgtatttgta gccgcgaata ctgcgatcgc ccccggcgaa gaaacgcaga tccggcggta
    10321 ctttgtcgaa atcaccggtt tcaatccagc ccagcgtgcc gcgtgtcaca aagcgatggc
    10381 gatcgtacag tgtgcggatc cagacgttct gcgcctggaa aacggagaaa tcgacatctg
    10441 aaccccaggc cgtgttggag tagtcgatag agtagcgttg cgagtcgccc caggttggca
    10501 tcaggccacc acgagaacgc gtgcggctaa tcatcacccc aggataaaac agcatcgtgg
    10561 tgttggtaat ttcaccctgg gtaaagtggt cgaggctcca gcgcaggtta atggcacgct
    10621 gccagccgct ggagagatcc cagtagcgag aagccaccag cgtagtggag tcagattcgg
    10681 tatcgttcag gtcagtgcgc ttaaaaccgc cctgcaccaa ataatattgt tccagtggat
    10741 tcttcagcag cggcatttta tagctgaagt cgagggtctg ttccggcgcg gaaatactgg
    10801 tactggtggt caaactgtga ccgtatgagt tcatccacgg ctttttccac gtcgctttca
    10861 cgcgcggtcc cacgtccgta gagtaaccga ccccggtttc gatggtgttt tctgttcgcg
    10921 gcgaaaccac gcccgtcaat ggtaatactt tcgtttcgcg cgctttatca aattgtggag
    10981 ccaccaccac cgagttaaac cagccggtag cagaaagtcg gcggttcagt tccgccagat
    11041 ctttcgattc atactcatcg ccctctttaa acggcaccag attttgcagg tattcatcgc
    11101 ggatttgtga tccttcaaag gtcacatgcc caaagcggta acgttcgcca ctgttataat
    11161 caatatccca gaaggcttta tgcaggccga gcgcaatgcc cagctgcgct ttggtaaatt
    11221 cgctatcgaa ataaccttta cgcaacgcaa tactggttaa ggactttttg aaattttcat
    11281 aatcgccctg gttcagtacc gtaccaatag ccgggcgagt atcgagcaat ttcaaatagt
    11341 ctttatcggt ccgcgcgccg ccgcgcaata ccacatcggt gccgccaatt aacaccggca
    11401 cacctggcgt gactttggcg atcaatacct gccgcccttt ctttggcggt ggacggagat
    11461 caaattcaat ggtcggctgg taataaccca gcgctttcag accttcacgg atggcatcat
    11521 cgacgcgtgc gcgaaagcga cggtctggcg tcacttcatc actttcaatc gtagaaagct
    11581 gcgcacgaac gttcttttcc agctgtcccg ataacccctc gacctgtaga cggacgttcg
    11641 cggcgacggc agatccgctt aagcagagta agcttacaca gcataactgt cggatatagc
    11701 gcacattttc tcctgaatat ccttttttcc tgcccctgga acgccgttaa acggcttaac
    11761 aaaaatccag taatatggat taaaaagcag actaaacccc aaatatttct tatgttttac
    11821 tttagaccta ttcacggtgg ttattgtgtg caaatacgcc tcttgttaca accttaaccc
    11881 caatgaccga ttttcgggag agcgacacca tgagtttatt tgataaaaag catctggttt
    11941 cccccgccga tgccctgcct ggacgtaaca ccccgatgcc cgtagccacg ctgcatgcgg
    12001 tcaacggtca ctcaatgacc aatgtacctg acggaatgga gattgccatt tttgcgatgg
    12061 gttgtttctg gggtgtggag cgtctgttct ggcagttacc cggcgtttac agcaccgccg
    12121 caggctatac cggcggctat acgccaaatc cgacttatcg ggaagtgtgc tccggtgata
    12181 cgggtcatgc cgaagcggta cgcattgttt acgatccttc cgtcatcagc tatgagcagt
    12241 tgctacaggt attttgggag aatcacgatc ccgcccaggg aatgcgtcag ggcaatgacc
    12301 acggcacgca gtatcgttca gcgatttatc cgctgacccc agaacaggat gccgcagctc
    12361 gcgccagtct ggaacgtttt caggcggcga tgcttgccgc cgatgacgat cgtcacatca
    12421 ccacggaaat cgctaacgcc acaccgtttt attatgccga agatgaccac cagcaatatc
    12481 tgcataaaaa cccatatggt tactgtggaa ttggcgtctg tctgccgccg gaagcatagc
    12541 gttacgggta caaatgtagt ttgttgataa agtgctcttt gtctatgcct gatgcggctt
    12601 aaacgactta ccaggcctac aaaatcgtcc aaattcaata tattgcaagg actgcgtagg
    12661 cctgatcggc atagcgcatc aggcagtatt gcatttatca gcagtctgaa ttttaaccct
    12721 ctggcgactt tacagtacct tacgctatac tagccactga aaatgccggt tcactttctt
    12781 cgaatcggct ttcaatgtgt atttcacaca aattaatcaa ctttcccttc cgaggatctg
    12841 gcctgaaagg tcggataaga tatgttaaac agtattttag tcatactctg cttgatcgct
    12901 gtaagtgcgt tcttctcgat gtccgagatc tcgcttgccg cctcacgcaa aatcaaactt
    12961 aaactgctgg ctgatgaagg caatataaat gcccaacgcg ttctgaatat gcaggaaaat
    13021 cccggcatgt tctttaccgt ggtccaaatc ggtctgaacg cagtggcgat tctcggcggt
    13081 atcgtcggtg atgcggcatt ttctccagct tttcacagcc tgttctcccg ctatatgtcg
    13141 gcagagctct ctgagcaact gagctttatt ctctctttct cgttagtgac tggcatgttt
    13201 atcctgtttg cggatttaac cccgaaacgc atcggtatga ttgcgccaga agcggtggct
    13261 ttgcgtatca tcaacccgat gcgcttctgc ctgtacgttt gcaccccgct ggtgtggttc
    13321 ttcaacggcc tggcgaacat gatcttccgt attttcaaac tgccaatggt acgtaaagat
    13381 gacatcactt ctgatgacat ctacgcggta gtggaagccg gtgcgctggc gggcgtgtta
    13441 cgtaaacagg aacacgagct gattgaaaac gtctttgagc tggaatcccg taccgttccg
    13501 tcttcaatga caccgcgtga aaacgtgatt tggtttgatc tccacgaaga tgagcaaagc
    13561 ctgaagaata aggtggcgga acatccgcac tctaagttcc tcgtctgtaa tgaagatatt
    13621 gaccacatca tcggttatgt cgattctaaa gacctgctga accgcgtgct ggctaaccaa
    13681 agcctggcac tgaacagcgg cgtacaaatt cgcaacacgc tgattgtgcc ggatacgtta
    13741 accctttcag aggcgttgga aagttttaaa accgcaggtg aagacttcgc ggtgatcatg
    13801 aacgagtacg cgctggtggt ggggatcatc accctcaacg acgtgatgac cacgctgatg
    13861 ggcgatctgg tcggtcaggg gctggaagag cagattgtcg cccgtgatga gaactcatgg
    13921 ctgattgacg gcggcacccc aattgacgac gtcatgcgcg tgctggatat tgacgagttc
    13981 ccgcagtcgg gcaactacga aaccatcggc ggctttatga tgtttatgct gcgtaagatc
    14041 ccgaaacgca ccgattcggt gaaattcgcc ggctataaat ttgaagtggt ggatatcgat
    14101 aactaccgca tcgaccagct gctggtgacc cggatcgaca gcaaggccac cgccctttcg
    14161 ccaaaactgc ctgacgctaa agataaagaa gaaagcgtcg cgtaaccccc agaaacatca
    14221 acggctcctg aatcaggagc cgtcttatta ctgcatagca ctttggttaa gccatctctg
    14281 tttgcagacg cataacctga cggttaactt cggacatcac tgacaaatgc agcttatcct
    14341 tcacttttgg gataagaatc ttacccttat caaattcaaa agcgccaacg tccttgatgt
    14401 ataaccgtcc acggaacagg atctttacgt acttcgccac ctgaagtggg ttgtaccgtt
    14461 ggaaaatttt cattgttatc tcctgctgag tattacgcct gtgcggtgcc acaatcggcc
    14521 caactattat gaggcgcaaa ttttaatgct tagtgactat agactatccg ggcaatgttt
    14581 ccaccgcgta taactttttt tacctaaagg ttacaattat tcagaattat ctttttaccg
    14641 aagcgcggtc ttcagtataa gcattcattt ttcatatgat ttgtgcgctt gaccgcaaac
    14701 tggcatcaca cttgcgggaa attcgataaa tagcacatat gattaaaact cagacccaag
    14761 tggtcggatc acctgcatat cataagaagg aaacaccatg accctacgca agattctggc
    14821 actcacctgc ctgctgttgc cgatgatggc ttccgcacat cagttcgaaa tcggtcagcg
    14881 agtgccgccg attggcatta ccgatcgggg cgagttggtg cttgataaag atcagtttag
    14941 ctacaaaacc tggaacagcg cgcagttagt gggaaaagtg cgagtactgc aacatattgc
    15001 tggtcgcacc tctgcaaaag agaaaaacgc gacgctgatt gaagcgatta aatcagcgaa
    15061 gttaccgcac gatcgttacc agaccaccac cattgttaac accgacgacg cgattccggg
    15121 ttccggcatg tttgtgcgca gcagtctgga gagtaataaa aagctttatc cctggtcgca
    15181 gtttattgtc gatagcaatg gtgtcgcgcg cggtgcctgg cagctggatg aagaaagttc
    15241 cgctgtagtg gtgctggata aagacggtcg cgtgcaatgg gccaaagacg gggcgctcac
    15301 accggaagag gtgcagcaag tgatggacct gctgcaaaag ttgctcaaat aatggcaata
    15361 acataaattc tggaaaaata attttcccct atcgttatct atgggatagg ggaattaagt
    15421 ctctgagttc ttacaccgaa ctaatactgg taacgctagt tgctcttttt taaacgcctc
    15481 aatggtcgat ttatatttct cgcaaatctt ttctttggta atattactat ctgcggccag
    15541 attcgcactt actatcgcat ccacttgttc ggctaattcg acttgttggc aacctgcatt
    15601 aaccagaaaa ttataaataa gagagtaatc ttcatcctcc aaaacaacag aggaaccatt
    15661 acgaaaacgt tcatcccctc catagatttt aagcgcaaag cccaaagatt ctatgactat
    15721 ggcaaaatta tcatcaatta ttttttcgta aatataccat ccatcgcatt ctttaattcc
    15781 accgcctggt atttggagtt caaatttacc ccgttttaat ttgcttacta catcctccaa
    15841 taaggcataa ccgaaagata cgtcaccaaa cgcctggcga atataacctt taatcctttt
    15901 tggtatgtca gactgttttt tctgcaaaat atcacaggca tacaatcttc tgttttgttc
    15961 taacggaaac tcgcggtttt tgaaatccat gaatgagtta tactcattta ttgttatggg
    16021 aatttcttcg tattcaaacc cactgcctcg caatgtcatt gtatattcag caccgttatc
    16081 tttaaggctg aaactgataa gaaaattatc catggcatta acagtttcat tttttgatga
    16141 aagcacttga taactatttt tatcaatctg tgttgcgcaa cgaaaggact cccgcaaagt
    16201 taacagacta tcataattcg aaacccaatc ttttttctta aatgcatgct gaatattatt
    16261 aaaaaaattg tttgaatgtg tttttgtatg cgatgacttt acaaataact ccaccgcacc
    16321 aataaaatta ttgtgtctga tcctgggtaa catatggcta ccttatttta tttggttatt
    16381 ttatgtcgcc attaaatatc tcattataga agtaaactat caaaaaacgc tcattattaa
    16441 aatgtaactt attagcaatt gaatttttgc actttaaata caatctcttt ttatttataa
    16501 ctttttaggt tgggataaat caggatggca gaatcaggaa atacactgtt tctgccatct
    16561 gaatttagta aatggacact ctgaaccccg gattcaggaa cgactcgcgc ggagtgtaat
    16621 ccagcggttt accctgccag tcgtgaacgt gcgctccggc agctgcagcc acagcatgtc
    16681 cagcggcggt gtcccaaata ttcgttggtc cgaagcgcgg gtacagctgc gcctgtcctt
    16741 ccgccaccag gcagaatttc agcgaagagc cgatggacgt ggtctgatgt tcgccaagct
    16801 gttgcagata ctctttcagc tccgcatctg catgggaacg gctgataacc accagcggcg
    16861 ggcgcgcatc gcggacctga atcagcttgc gcacaccgca ctcttctttc caggccttgc
    16921 cttctgccgc gctgtacatt acgttcatta ccggcgcata caccacacct aaaatcggtt
    16981 tgccatggtc aatgagcgca atgttaacgg tgaattcgcc attacgttta ataaactctt
    17041 tagtaccatc cagcgggtct accagccagt aacgctgcca gtgctgacgg acttcccaac
    17101 cgggaggatc ttcttcagaa aggaccggaa tatccggtgc cagcgtacgt aaaccgtcca
    17161 tgataacggt gtgagcggca atatccgctg ccgttaccgg agaattgtcc gctttgctga
    17221 cgacgtccat cggtttcgtc ccgtcgtaga cctgcataat ggcatcgcct gcattccgtg
    17281 caagctggca tacttgatct aacatttctc cacctcgtct ctgtgagcgg tgttaactta
    17341 ttgttttact tataccctat cgttaatgaa tgcgccaact gtgatagtgt catcattttc
    17401 aaagcgtaaa attgtggcat tcttcactgt tctataagta aggcgtttat tctccctttt
    17461 ctttcgtatt cccgatgata aaaggatgtc cctgatgatt aagtttagcg caacgctcct
    17521 ggccacgctg attgccgcca gtgtgaatgc agcgacggtc gatctgcgta tcatggaaac
    17581 cactgatctg catagcaaca tgatggattt cgattattac aaagacaccg ccacggaaaa
    17641 attcggactg gtacgtacgg caagcctgat taacgatgcc cgcaatgaag tgaaaaacag
    17701 cgtactggtc gataacggcg atttgattca ggggagtccg ctggccgatt acatgtcggc
    17761 gaaaggatta aaagcaggtg atgttcatcc ggtttataag gcgctgaata cgctggatta
    17821 tacggtcggt acactcggca atcatgaatt taactacggt ctggattacc tgaaaaatgc
    17881 gctggcggga gcgaaattcc cttatgtaaa tgccaacgtc attgacgcca gaaccaaaca
    17941 gccaatgttt acaccgtatt taattaaaga taccgaagtg gtcgataaag acggaaaaaa
    18001 acagacgctg aagattggct atattggcgt cgtgccgccg caaatcatgg gctgggataa
    18061 agctaattta tccggaaaag tgacggtgaa tgatattacc gaaaccgtgc gcaaatacgt
    18121 gcctgaaatg cgcgagaaag gtgccgatgt cgttgtcgtt ctggcgcatt ccgggctgtc
    18181 tgccgaataa agtaatggcg gaaaactcag tttattacct cagtgaaatt ccgggcgtta
    18241 acgccattat gtttggccat gctcacgccg ttttcccggg taaagatttt gctgatatcg
    18301 aaggggctga tatcaccaaa ggcacgctga atggtgttcc ggcggtaatg ccgggcatgt
    18361 ggggcgatca tcttggggtg gtcgacttac aactcagtaa tgacagcggt aaatggcagg
    18421 tgacgcaggc gaaagcggaa gcacggccga tttacgacat cgccaataaa aaatccctcg
    18481 cggcggaaga cagcaagctg gtagaaacac tcaaagccga tcacgatgcc acacgccagt
    18541 tcgtcagcaa gccaatcggt aaatctgccg acaatatgta tagctatctg gcgctggtgc
    18601 aggacgatcc gaccgtgcaa gtcgtgaaca acgcgcaaaa agcgtatgtc gaacattaca
    18661 ttcagggcga tccggatctg gcaaaactgc cggtgctttc agctgccgca ccgtttaaag
    18721 ttggtggtcg caaaaatgac ccggcaagct atgtggaggt ggaaaaaggc cagttgacct
    18781 tccgtaatgc cgccgatctt tatctctacc ccaatacgct gattgtagtg aaagccagcg
    18841 gtaaagaggt gaaagagtgg ctggagtgct ccgccggaca gtttaaccag attgctccta
    18901 acagcacgaa accacagtca ctcatcaact gggatggttt ccgcacttat aactttgatg
    18961 ttattgatgg tgtgaattat cagattgatg ttacccaacc cgcccgctat gacggcgagt
    19021 gccagatgat taatgccaat gcagagcgga ttaagaacct gacctttaat ggcaagccga
    19081 ttgatccgaa cgctatgttc ctggttgcca ccaataacta tcgcgcttac ggcggcaaat
    19141 ttgccggtac gggcgacagc catatcgctt ttgcttcacc ggatgagaac cgctcggtgc
    19201 tggcagcgtg gattgctgat gagtcgaaac gtgcggggga aattcatccg gcggcagata
    19261 acaactggcg tttagcaccg atagctgccg ataagaaact ggatatccgt ttcgaaactt
    19321 ccccgtcaga taaagccgca gcgtttatta aagagaaagg gcagtatccg atgaataaag
    19381 tcgcgaccga tgatatcggg tttgcgattt atcaggtgga tttgagtaag taaaacactt
    19441 ctttttcggc ctataaatca accgcatccg gcatttattg gcggatgcga tgctgccgta
    19501 tcttatccac cctacaagcc atgaaccgta gaccggataa gcgcagcgca tccggcagtt
    19561 atgccgcacg ttcatcccgc accgccaaca cctcgggcaa attcaactca atccagtccg
    19621 ccagtgcagc aaccttttcg ctcacctgct cgcccagcgg cgtgaggcta tattccacat
    19681 gcggcggcac caccgaatac gcgatacggt taagaaaacc atcctgttct aacgcctgta
    19741 acgactgcgc aagcatcttt tcactcaccc caccgatttt gcgccgcagg tcgctaaagc
    19801 gatgagtacc ttcgcgtagc gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt
    19861 tcaacacctc gcgcgacggg cactgttccg caaagagatt accctctttc agttgttgtg
    19921 acaggctaac ctgactcatt tcatacttac ctttttgtat gtacttacta aaagtaagtt
    19981 taggtgttag cgtatttaaa cacaagacaa aacgatggag acttcccatg atcgctatta
    20041 ctggtgccac tggccaactt ggtcactata ttattgaatc cttgatgaaa acggttcctg
    20101 ccagccaaat agtggctatc gttcgtaatc cggcaaaagc ccaggccctg gcagcacaag
    20161 gcattaccgt gcgtcaggct gactacagcg atgaagccgc actgacatct gcacttcagg
    20221 gagtggaaaa actactgctg atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc
    20281 gtaatgttat taatgccgca aaggcggctg gcgtgaaatt tatcgcttat accagcctgc
    20341 tacatgcaga tacctccccg ctcggcctcg ccgatgagca catcgagacg gagaaaatgc
    20401 tggctgattc tggcatcgtt tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc
    20461 tcgccagcgc cccggcagca ctggaacacg gcgtatttat cggtgcggcg ggcgatggca
    20521 aaatcgcctc agcaacgcgg gcagattatg cgacagctgc ggcacgcgtg attagcgaag
    20581 ccggtcacga aggcaaggtt tacgaactgg cgggcgatag tgcctggacg ttgacacagt
    20641 tagcggcaga gctgaccaaa cagagcggca aacaggttac ctatcaaaat ctgagcgaag
    20701 ccgatttcgc cgcggcactg aaaagcgtcg gactgcccga cggactggcg gatatgctgg
    20761 cggattctga cgttggcgca tcgaaaggtg gtctgtttga taacagcaaa acgcttagca
    20821 aattgattgg ccgcccaacg acaacgttag ccgaaagcgt aagccatctt tttaatgtta
    20881 ataactagtt aattaaagtg gcatcctccc gcatcctctc tgataatgac gggatgccgg
    20941 gagcaatcat gtctgcttcc tgaacgtttc ttctgacaga ccaatggatg ccagtaatga
    21001 ttagcggcgt gctgtacgcc ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc
    21061 cgttgatcgt gccggaatac ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag
    21121 ggttaattgc gctacccatt gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc
    21181 gggactggct taccgccttg atgctcacta tgatgggcaa cctcatttat tacttctgcc
    21241 ttgccagtgc cattcaacgt actggcgcgc ctgtttccac gatgattatc ggcaccctgc
    21301 cggtggtgat tcctgtcttt gccaatctgc tttatagcca gcgcgacggc aaactcgcgt
    21361 ggggaaaact cgccccggca ctgatttgta ttggcatcgg cctggcgagt gtgaatattg
    21421 ctgagttaaa ccacggactc cccgattttg actgtgcacg ttatacctct ggcatcgtgc
    21481 tagcgttagt ttccgtggtc tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc
    21541 gggaaaatcc cgacaaacat ccgatgatgt gggcgacggc gcaggcgctg gtcacgctgc
    21601 cagtttctct catcggctat ctcgtcgcct gttactggct gaatatacaa acgccggact
    21661 tctccttacc ttttggcccc cgtccg
//
LOCUS       DADLSA010000055        21411 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.055, whole genome shotgun sequence.
ACCESSION   DADLSA010000055 DADLSA010000000
VERSION     DADLSA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.055"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            41..850
                     /locus_tag="JN691_002111"
     CDS             41..850
                     /locus_tag="JN691_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7243559.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQLIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKICGRCNGNRFSRLPTTLARHHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAETQLRKVTRQMIFAEDGDMMLAFFKKYG"
     gene            944..1201
                     /locus_tag="JN691_002112"
     CDS             944..1201
                     /locus_tag="JN691_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002432029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243560.1"
                     /translation="MQGEKQQPYFFNPGMTVEQLEDWLEQQKLHLSRYNRLVKEKAEL
                     EERLSDISVEIERVSAGGFNGKLSFPWESSSLLRNHQQGSV"
     gene            1281..1667
                     /locus_tag="JN691_002113"
     CDS             1281..1667
                     /locus_tag="JN691_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243561.1"
                     /translation="MSDPISGTGLAGGALTGASVYGLLTGTDYGVVFGAFAGAVFYIA
                     TAADLSASRRLAYFIVSYIAGILCSGLVGSKLAHLTGYSDKPLDAIGAVIVSALTVKI
                     LTFLNNQDIGSLVALITRRGGSGGAK"
     gene            1654..1935
                     /locus_tag="JN691_002114"
     CDS             1654..1935
                     /locus_tag="JN691_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAY7243562.1"
                     /translation="MELNDPTATINALLCACVVITLMFYRRGDSRHRPWVSRLAWLIT
                     VTYSAVPLAYLCGIYPHSSWPIIVANTIFLSVLVAVRGNVARLVDHLRH"
     gene            1935..2552
                     /locus_tag="JN691_002115"
     CDS             1935..2552
                     /locus_tag="JN691_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HAY7243563.1"
                     /translation="MNQQLFQKAAGISAGLAARWFPHIDAAMKEFGITAVNDQAMFIA
                     QVGHESAGFTSLVESFNYSVDGLKKTFSKRLTPYQCEMLGRVDGKQVAHQPQIANLVY
                     GDRMGNNSQGDGWKYRGRGLLQITGRENYAKCGAALKLDLISTPELLTQEKHAARSAA
                     WYFTLRGCLMYSGDVVRVTQIINGGQNGLADRNSRYNKARAALLV"
     gene            2549..3079
                     /locus_tag="JN691_002116"
     CDS             2549..3079
                     /locus_tag="JN691_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HAY7243564.1"
                     /translation="MTAVFAFVQARWKTIIVLLMLAGAFLAGNIWSERGWQKKWADHN
                     SMESSQEANAQTAARWIEQGRIIARDEAVKDAQAQAAKSAATAAGLSATVSQLRTEAT
                     KLAARLDAAKHTADLAAAVRSKTTDAGMFADMLGDIAAEAKRYAGIADERYTAGMTCE
                     RIYDSLRESNNAIPHN"
     gene            3146..3421
                     /locus_tag="JN691_002117"
     CDS             3146..3421
                     /locus_tag="JN691_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243565.1"
                     /translation="MSKQGYAVSGHSDGLADILSIVIVLAVEHWIIASLIVGLIMYLV
                     GASINENAQTNAGAIASGIIGVCGQFIFGFGVLASIIGIVKFIWTHT"
     gene            3486..3683
                     /locus_tag="JN691_002118"
     CDS             3486..3683
                     /locus_tag="JN691_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016150493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243566.1"
                     /translation="MTVVLTAKQIEDLAAFAKEDGQPQYTITTGTIPEFEADDGEIIP
                     EYTGLIAYSESLEHGVLQLDD"
     gene            3747..4097
                     /locus_tag="JN691_002119"
     CDS             3747..4097
                     /locus_tag="JN691_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003034766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7243567.1"
                     /translation="MPPRTPKACRVRGCRNTTTDPSGYCENHKGEGWKSYKPGQSRQQ
                     RGYGTKWEVIRERILKRDKGLCQNHLRQAIVKQASCVDHIKAKAHGGTDEDSNLESLC
                     WSCHAAKTARERLK"
     gene            4224..4718
                     /locus_tag="JN691_002120"
     CDS             4224..4718
                     /locus_tag="JN691_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003832359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY7243568.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            4715..6448
                     /locus_tag="JN691_002121"
     CDS             4715..6448
                     /locus_tag="JN691_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY7243569.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     SRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTDPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDAVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIIQNYTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKTIPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            6460..6642
                     /locus_tag="JN691_002122"
     CDS             6460..6642
                     /locus_tag="JN691_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243570.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPVGFVVAGALCLFWSWL
                     VARYLDRTQQSVGGGK"
     gene            6642..7883
                     /locus_tag="JN691_002123"
     CDS             6642..7883
                     /locus_tag="JN691_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY7243571.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLTESVGMLPCNLYHLNGGLKQRSTGERLHKLISSHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPGCVVPKLNSSWEPVYQVTFPDGSTDVLT
                     QEDIWHVRTLTLDGLVGLNPVAYAREAILLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGPDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKLGIYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            7861..8511
                     /locus_tag="JN691_002124"
     CDS             7861..8511
                     /locus_tag="JN691_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAY7243572.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSVLNALKNLNF"
     gene            8526..9731
                     /locus_tag="JN691_002125"
     CDS             8526..9731
                     /locus_tag="JN691_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY7243573.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDARSQTATSKLGLIEPFMGEIYGNPQATQKMLDDTFFNVEAWINSELATE
                     FAEQEEIAFTTGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATTVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDTEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTCILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            9782..9982
                     /locus_tag="JN691_002126"
     CDS             9782..9982
                     /locus_tag="JN691_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243574.1"
                     /translation="MILKQDLKWSPDGMRVEVIQAGEYDDGALPARVQEIALQAGLAE
                     RGISAKSSKAAKEKKATTSKEG"
     gene            9985..10308
                     /locus_tag="JN691_002127"
     CDS             9985..10308
                     /locus_tag="JN691_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY7243575.1"
                     /translation="MLLTMEEIKAQLRLDEDFDTDDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            10305..10715
                     /locus_tag="JN691_002128"
                     /pseudo
     CDS             10305..10715
                     /locus_tag="JN691_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
     gene            10690..11196
                     /locus_tag="JN691_002129"
     CDS             10690..11196
                     /locus_tag="JN691_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243576.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            11193..11753
                     /locus_tag="JN691_002130"
     CDS             11193..11753
                     /locus_tag="JN691_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243577.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLKLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELQTLA
                     IDVDYLEPGNGPDGDIEHHTEITLPS"
     gene            11762..11932
                     /locus_tag="JN691_002131"
     CDS             11762..11932
                     /locus_tag="JN691_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HAY7243578.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            11916..13412
                     /locus_tag="JN691_002132"
     CDS             11916..13412
                     /locus_tag="JN691_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7243579.1"
                     /translation="MTISFNTIPSNTLVPLFYAEMDNSAANTAQDSGASLLIGHANNG
                     AEIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPEATGAAATV
                     TLTVNGAATETGTVNVYVGRTRVQAPVTNGDNVTTIASSIQDAINAVPALPFTASSSA
                     GVVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAG
                     DQFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPACPTQTGELVGMLPAPKG
                     KRFTMTEQQTLLFHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            13412..13768
                     /locus_tag="JN691_002133"
     CDS             13412..13768
                     /locus_tag="JN691_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7243580.1"
                     /translation="MARIGGTCYFKMDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVSKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            13768..14037
                     /locus_tag="JN691_002134"
     CDS             13768..14037
                     /locus_tag="JN691_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HAY7243581.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            14179..16014
                     /locus_tag="JN691_002135"
     CDS             14179..16014
                     /locus_tag="JN691_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7243582.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGSPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILILGLMGVGSAISIVSRIMSMTPIGMIATAIAMAAGLIITNWDVVGP
                     YFKKLWETIGPYFEAGWELLKKVFAWSPLGMVINHWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYPVVDLAQSNNASGAIGPNPFMIN
                     KATAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            16075..17403
                     /locus_tag="JN691_002136"
     CDS             16075..17403
                     /locus_tag="JN691_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HAY7243583.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVIGNASIMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSDVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNAAVGQATTPAQSTCWPSATHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAETWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            17400..18479
                     /locus_tag="JN691_002137"
     CDS             17400..18479
                     /locus_tag="JN691_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HAY7243584.1"
                     /translation="MNDNVTLRVNGREWNGWTLVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATTVRYDARSVITGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            18479..19027
                     /locus_tag="JN691_002138"
     CDS             18479..19027
                     /locus_tag="JN691_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HAY7243585.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     IEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPKARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMGA"
     gene            19027..19452
                     /locus_tag="JN691_002139"
     CDS             19027..19452
                     /locus_tag="JN691_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7243586.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMIDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            19439..20497
                     /locus_tag="JN691_002140"
     CDS             19439..20497
                     /locus_tag="JN691_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HAY7243587.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFVRLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATAASGYMRLEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRVPITCSTTGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEDLETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYLQGELKVSRISEAISGANGEYSHQLLAPAD
                     NISIAKNELAVLGTISWT"
     gene            20488..21072
                     /locus_tag="JN691_002141"
     CDS             20488..21072
                     /locus_tag="JN691_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HAY7243588.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAQSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRQDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNSTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            21076..>21411
                     /locus_tag="JN691_002142"
     CDS             21076..>21411
                     /locus_tag="JN691_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243589.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTLATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGAE"
BASE COUNT         5052 a         5261 c         6374 g         4724 t
ORIGIN      
        1 gcgctggcaa taggcgtact ggcgtaagtg gagaacgagc atgaaccttg aagccttacc
       61 aaaatattac tccccaaaat ctccaaaact gagcgatgac gcaccggcga caggctcggg
      121 tggtttaacg attacggatg tgatggctgc gcaggggatg gtgcagtcga aagcaccgct
      181 tggatttgcc ttattcctgg caaaagttgg tgttcaggat cctcagtttg cgattgaagg
      241 tctgctcaat tacgcgatgg cactggataa tccgacattg aataaattga gtgaagaaac
      301 ccggttacag ctcatccctt accttgtgaa ttttgccttt gctgattatt ccaggtctgc
      361 ggcaagtaag gctcgctgtg agcattgtgc tggtactgga tttcataatg tattgcgcga
      421 agtggtgaaa cactccagaa gcggtgaatc tgttatcaag gaggagtggg tgaaggaact
      481 atgtcagcat tgtcatggta agggagaagt cagcacagcg tgcagagggt gtaagggtaa
      541 aggtattgtc ctggatgaaa aaaggacccg gcttcatggc acgcctgttt ataagatttg
      601 tgggcgttgc aatggaaacc ggtttagccg tttgccaacc acactggcgc ggcatcatgt
      661 ccagaagctg gtaccggacc tgacggatta tcagtggtac aaaggatatg cagatgtcat
      721 tgataaactg gttacaaagt gctggcagga agaagcatat gcagagacac aattgagaaa
      781 agtgacaaga caaatgattt tcgccgaaga tggcgacatg atgcttgcat ttttcaaaaa
      841 atatggttag gattctccta acgatgggct ttgtatgtct gccgttaacg aaatcataac
      901 aaacctcgct tcggcggggt ttttgctttt ctggaggtca ataatgcagg gcgaaaagca
      961 gcagccatat ttttttaacc ctggtatgac tgttgaacag cttgaagact ggctggagca
     1021 gcaaaagctt catctaagcc gctataaccg tctggtaaaa gaaaaagcag agcttgaaga
     1081 acggctcagt gatatttctg tggaaattga acgagtgtct gctggtggtt ttaacggaaa
     1141 gttgagtttc ccctgggagt caagttcgct tctgagaaat catcaacagg gtagtgtttg
     1201 actgaaataa taaatagact gtcattaaga tcccttcccc tcatatctga gaggaccaac
     1261 agcaattaag agggggctaa atgtccgatc cgatttccgg tactgggctg gctggtggtg
     1321 ccctgacggg tgccagtgtt tatggactgc tgaccggaac tgattacggc gttgtatttg
     1381 gcgcatttgc aggggctgta ttctacatag caacagcagc agatctgagt gcatcgcgcc
     1441 gactggcata ttttatcgtg tcatatattg ccgggatcct ttgctctggg ttggttggct
     1501 ccaagctggc gcacttgacc ggatacagtg ataaacctct ggatgctatt ggtgccgtaa
     1561 tcgtctctgc tttaaccgtt aaaatcctga cgttcctgaa taatcaggat atcggctcgc
     1621 tggtggcgct cataacgcgc cggggaggtt caggtggagc taaatgaccc gacagcaact
     1681 ataaatgcgc tgttatgtgc ttgtgttgtt attactctga tgttttatcg tcgtggtgat
     1741 tcgcggcatc gtccttgggt ttcacgttta gcctggctga ttactgttac atacagtgct
     1801 gttccgttgg cctatctctg tgggatttat ccccattcct catggcccat tatcgtggcg
     1861 aacactattt ttctttccgt gctggtggcc gtcagaggca acgttgcacg tctggttgat
     1921 catctgaggc actaatgaac caacaattat ttcaaaaggc ggctggtatt agcgccgggc
     1981 tggctgcgcg ctggtttccg cacattgatg ctgcaatgaa agagtttgga atcacagcag
     2041 ttaacgatca ggccatgttc attgcacaag ttgggcatga atctgctggt tttacctcgc
     2101 tggtggaaag cttcaactac tcggtagacg ggctgaagaa aacctttagt aaacgcctga
     2161 cgccttatca gtgtgaaatg ctggggcgtg tcgatggtaa gcaggtagcc caccagccgc
     2221 aaatagccaa tctggtttat ggtgaccgca tggggaataa cagccagggt gacggctgga
     2281 aatatcgcgg tcgtggcctg cttcaaatca ccggccgcga gaactacgcc aaatgcggtg
     2341 cggcgctgaa gttggatctg atcagcacac cagagttgct gacacaggag aaacatgcag
     2401 cccgttctgc tgcatggtat ttcacattac gtggttgtct gatgtattca ggtgatgttg
     2461 tccgtgtaac gcagatcatc aacggtggcc agaatggact ggctgacaga aatagtcgtt
     2521 ataacaaagc gcgggcggcg ttgctggtat gacagcggtc tttgcttttg ttcaggcgcg
     2581 gtggaaaaca atcattgttt tgctgatgtt ggctggtgca tttcttgccg ggaacatctg
     2641 gagtgagcgg ggctggcaaa agaagtgggc tgaccacaat agcatggaat cttcacagga
     2701 agcgaacgcg cagactgccg cacgctggat tgaacaaggg cgcataattg cccgtgatga
     2761 ggctgtaaaa gatgcacaag cacaagccgc taaatctgct gccactgctg ctggcctgtc
     2821 tgccactgtt agccagttgc gtaccgaagc aacaaagctt gccgcacgcc tggacgccgc
     2881 aaagcacacc gctgatcttg ccgctgccgt ccgaagcaaa acaaccgacg ccggaatgtt
     2941 cgccgacatg ctcggagata ttgcagcaga agctaaacgt tatgctggaa tcgctgacga
     3001 acgctacacc gccgggatga cgtgtgaacg tatttacgac tcgttgagag agtcaaataa
     3061 cgctatcccc cacaattgat tttttatcat attcgaacga ctatgaaggg tgtaaacaat
     3121 tataatccag tgatgaattt ttcaaatgtc aaagcaagga tatgcagtga gtggtcatag
     3181 tgatggttta gcagatattt tgagcattgt aattgtatta gcagtggaac attggattat
     3241 cgcatctctc atagttggtt tgattatgta tctagtgggg gcgagcataa acgaaaatgc
     3301 tcagacaaat gccggtgcca ttgcatcggg aatcataggg gtatgtggtc aatttatttt
     3361 tggttttggc gttctggcta gcattattgg gattgttaaa ttcatttgga cccatacctg
     3421 acagcatcac tttagtcaat catagcctcg caatagcggg gctttttaac aactgaggta
     3481 tgagcatgac agtagttctt acagctaagc agattgaaga cctagcagca ttcgcgaaag
     3541 aagatggtca gccccaatac accatcacca ctggtacaat cccggaattc gaagcggatg
     3601 atggcgagat tatccctgaa tacacagggc tgatcgctta ctcagagtca cttgagcacg
     3661 gtgtattgca actcgacgat tagtggcatt cagcaggcat tcattaagtg tctgcgataa
     3721 tgctatacgt tttttgggga gtgaatatgc caccgcgcac accaaaggct tgccgtgttc
     3781 gcggctgtcg caacaccaca acagacccat ctggctactg tgaaaatcac aaaggtgaag
     3841 gctggaagtc ctacaagcct ggtcaatcac ggcagcagcg aggatacgga acaaagtggg
     3901 aagtcatccg ggagcggata cttaagcgcg acaaagggct gtgtcagaac catctacggc
     3961 aggcgatagt gaagcaggcg tcctgcgtgg accacatcaa ggcgaaggcc cacggcggta
     4021 ctgatgaaga cagcaacctt gaaagcctgt gctggtcgtg tcacgcagcg aagaccgcgc
     4081 gtgagcggct caagtgataa ttaatgtcat catcagactg gggaggggga ggtcaaatct
     4141 ctgtgaccgc gcgccttccg gactgcccgc cccatcgttt ttttataccc gcgaaaaatg
     4201 aaatttaacc aggagtgccg catatggctg gaacggcggg gcgttccggg cgtcgcccca
     4261 agccaacggc gcgcaaggcg ctggccggaa accccggcaa gcgagccctg aataaagatg
     4321 aacctgtttt tacgcccatc aaaggtgttg agccaccgga gtggttcgct gaagaagatc
     4381 tccctctcgc tacgatcatg tggcaactga caactaaaga actctgcggt cagggcctgc
     4441 tgtgcgtgac tgacctcgcg gtgcttgagc ggtggtgcgt ggcctacgag ttctggcgac
     4501 gtgccgtgaa aaatattgcc agacagggca acaccatcac cggtgcaatg ggcggtatgg
     4561 tcaaaaatcc ggagctgacc gccaaaaaag aacaggagtc cgagatgagc agtacggggg
     4621 caatgctcgg actcgacccc agcagccgcc agcgtctgat tggcctggcg gggcagaaga
     4681 aagccactaa cccgtttctg aaaatcatcg aatcatgagc cggaaatctt accccaacgt
     4741 aaatgctgcc aatcagtatg cccgtgatgt cgtgcgcgga aagattgtgg cctgccagtt
     4801 tgtgattcag gcctgccagc gccatcttga tgacctgatg gcggaaaaaa gtaagtcgtt
     4861 tcgttaccgc ttcgacaagg acctggctga acgggccgcg aaatttattc agctgttgcc
     4921 acacaccaag ggtgagtggg cattcaaacg gatgcccatc acgctggagc cgtggcagct
     4981 ctttgtgatc tgctgcgcgt ttggctgggt caataaaggc tcccggctgc gccgcttccg
     5041 tgaggtgtat accgaaatcc cccgtaagaa cggcaaatcg gcaatctctg ccggtgtcgc
     5101 cctgtattgt tttgcctgtg ataacgagtt cggcgcggaa gtgtattccg gtgccacgac
     5161 ggagaagcag gcatgggaag tctttcgtcc ggcacgactg atgtgtaaac gcacccccat
     5221 gctgacggaa gcgttcggga ttgaggttaa cgcctcaaac atgaatcgtc cggaggatgg
     5281 cgcgcggttt gaaccgctga tcggtaaccc cggtgatggt tcatcacccc actgtgcggt
     5341 ggtggatgaa tatcacgagc acgccaccga tgcgctttac accacgatgc ttaccgggat
     5401 gggggcgcga cgtcagccac tgatgtgggc tatcaccacc gccgggtaca acattgaggg
     5461 gccgtgctac gacaagcggc gggaagtcat cgagatgctc aacggctcgg tgcctaacga
     5521 tgaactgttc gggatcatct ataccgttga tgaaggtgac gactggaccg acccgcaggt
     5581 gctggaaaaa gccaatccaa atattggcgt gtcggtttat cgcgaatttt tgttaagtca
     5641 gcagcagcgt gcgaaaaata acgcccgtct ggcaaacgtc tttaaaacaa aacacctcaa
     5701 tatctgggtg tcggcgcgtt cggcgtattt caacctggtg agctggcaga gctgcgagga
     5761 taaatcactg acccttgagc agttcgaggg gcagccgtgc attctggcct ttgacctggc
     5821 gcgtaagctg gatatgaaca gcatggcgcg actttatacc cgcgagattg acggtaaaac
     5881 gcattactac agtgtggccc cgcgtttctg ggtaccgtat gacgcggtgt atagcgtcga
     5941 gaaaaatgaa gatcgccgga cagccgaacg ctttcagaaa tgggtggaaa tgggcgtcct
     6001 gaccgttacc gatggtgcag aggtggatta tcgctacatc ctcgaagagg ccaaagcggc
     6061 gaacaaaatc agcccggtca gcgagtcacc catcgacccc ttcggggcga ccgggctgtc
     6121 acatgacctt gctgatgaag acctgaaccc cgtcaccatc attcagaact acaccaacat
     6181 gtccgacccg atgaaagagc tggaagcggc aattgaatcg gggcgctttc atcatgatgg
     6241 caatcccatc atgacctggt gtatcggcaa cgtggtcgga aaaaccattc cgggtaacga
     6301 tgatgtggtg aagcccgtca aagagcaggc ggaaaacaaa atcgatggtg cagttgcgct
     6361 gattatggcg gttggcagag ccatgctgta cgagaaagaa gacacgctgt ctgaccacat
     6421 tgagtcctac gggatccgct cgctttaact gaggtaatta tgatcatgct gattctcgcg
     6481 cctctggtag gcgtgctggg ggcgcttttg ctggcgtatg gtgcctggct gatttatccc
     6541 ccggtgggtt ttgttgttgc cggggcgttg tgcctgttct ggtcgtggct ggtggcgcga
     6601 tatctcgacc gtacacagca gtctgtcggc ggaggtaaat agtgttcttt tcgggattat
     6661 ttcaacgaaa aagtgacgca ccggtgacca cgccagcaga gctggcggat gctatcgggt
     6721 tgtcctacga cacctatacc ggaaagcaga tcagcagtca gcgggccatg cgactgacgg
     6781 cggttttttc ctgtgttagg gtgctgacgg agtcggtcgg gatgttgccc tgcaacctgt
     6841 atcacctgaa tggcggtctg aagcagagat ccactggcga acgtctgcat aagctgatct
     6901 cctcgcatcc taatggctat atgacgccgc aggagttctg ggagctggtg gtcacctgtc
     6961 tgtgcctgcg gggcaacttt tacgcctaca aagtgaaagc atttggtgaa gtggctgaac
     7021 tgctgcccgt cgatcccggc tgtgtggtac cgaagcttaa cagtagctgg gagccggtct
     7081 atcaggtcac attcccggac ggctcaacgg atgtgctgac ccaggaggat atctggcatg
     7141 tgcgcacgct gacgctggac ggtctggtgg gactgaatcc cgtcgcctat gcccgcgagg
     7201 caatattgct ggcagcagcg actgaagaac acggtgccag actgttcagc aatggcgcgg
     7261 tgacgtccgg tgtgttgcgt acagaacaga cgctgtcgga tcaggcttat gagcgcctga
     7321 agaaagattt tgaggagcgt cacaccgggc ttggcaatgc tcaccgcccg atgatccttg
     7381 agatggggcc ggactggaag tcgatggcat tgaacgccga ggacagccag ttcctggaaa
     7441 cccgcaagtt tcagcttgaa gaaatctgtc gtctgttccg ggtgccattg catatggtgc
     7501 agaacaccga tcgcgccacc ttcaacaata tcgaagagct ggggctggga tttatcaact
     7561 attcactggt gccgtatctg acccgcattg agcagcggat caacaccgga ctggtacgaa
     7621 aaagtaagct gggcatttat tacgccaaat ttaacgccgg ggcgttactg cgcggggata
     7681 tgaagtcccg ttttgaagcc tacgccaccg ggatcaactg gggaatttac tctcccaatg
     7741 actgccgcga cctggaagat atgaatccgc gtcccggtgg tgatgtctat ctcacaccga
     7801 tgaacatgac cacgaaaccc tccgatggca gtaaagccgg taagcagaag gataacgcca
     7861 atgcagacga aacaacgtct tgatgtaccg ctgagtctga aatctgtcag tgactccggt
     7921 gagtttgaag ggtatggctc cgtctttggt gtaaaggaca gccacgatga tgtggtgatg
     7981 tccggggcat ttgctgcttc cctgcgggcg tggagtgaca gaaaagcgtt acctgcgctg
     8041 ctctggcagc accgcatgga tgaacccatc ggtgtttata ccgaaatgaa ggaagacgat
     8101 gtcgggcttt acgtcagggg acggttgctc attgatgatg atcccctcgc aaaacgcgca
     8161 catgcacaca tgaaggccgg ttcgttaacc ggcctttcta ttgggtacgt cctgaaagac
     8221 tgggaatacg accggagtaa agaagccttt ctgctgaaag aaatcgacct ctgggaagtc
     8281 agtctggtga cgttcccgtc taacgacgag gcgcggatca gcgacgtcaa gaacgcgctg
     8341 gcccgcggtg aaatccccga acagaaaaaa atcgaaagag tcctgcgtga tgtcggactc
     8401 tcccgtaccc aggccaaagc attcatggcc gggggctatg gcgcactgtc cctgcgcgac
     8461 gctgaggatg tgggctctgt actgaatgca ctgaaaaatc tgaacttcta atcaggagaa
     8521 atacgatggc ggttgatatt aaagatgtgg aacaggtcgc gcaggagctg cagcagaagt
     8581 ttgacgactt caaagcaaag aacgacaagc gcgtggatgc gattgagcag gaaaaaggca
     8641 aacttgccgg gcaggtggaa accctgaacg ggaaactcag cgagctggaa aacctcaaaa
     8701 gcgatcttga aaaagagctg cttgagctga aacgtccggc aggtggtgcg caaaataaac
     8761 tggccaccga gcataaagaa gcgtttgtgg gcttcctgcg taaaggccgt gaagatggtc
     8821 tgcgcgatct ggagcgcaag gcattacagg tgggcaccga tgaagacggc ggctatgccg
     8881 tgccggaagc actggatcgc aacattctca ccctgctgaa agatgaagtg gtgatgcgcc
     8941 aggaagccac ggtgatcacc gttggcggtt ccgactacaa aaaactggtg aatctgggcg
     9001 gcacggcttc cggatgggtt ggcgagactg acgcgcgctc ccagactgcc acctcaaaac
     9061 tgggactgat tgaacctttc atgggggaaa tctacggtaa cccgcaggcc actcagaaaa
     9121 tgctggatga taccttcttc aacgtggagg cctggatcaa cagcgagctg gcgaccgaat
     9181 ttgccgaaca ggaagaaatt gcctttacca ccggtgatgg caccaagaag ccaaaagggt
     9241 tcctggcgta tgaatccacc gatgaaaccg ataaggttcg tgcgttcggt aaacttcagc
     9301 atattgtatc cggtgaagcg acgacggtga ccgcagacgc cattatcaaa ctgatttaca
     9361 cgctgcgtaa ggcacaccgc actggcgcga agttcatgat gaacaacaac agcctgtttg
     9421 ccatccgtct gctgaaagac accgagggta actatctgtg gcgtccgggg ctggagctgg
     9481 ggcaaccgtc ctctctggcg ggttacggta tcgctgaaaa cgaacagatg ccggatatcg
     9541 ccgctgatgc gaaagctatt gcatttggta acttcaagcg gggttacacc atcgttgacc
     9601 gtatcggtac ctgcattctg cgtgatccgt acaccaataa accgtttgtc ggtttttata
     9661 caaccaagcg caccggcggc atgctggtcg attcgcaggc catcaaactg ctgaagattg
     9721 cagcggcgta atcactcagg ggcgcggaac cgcgccccct gttctgacag gtgaaagaat
     9781 catgatcctg aaacaagatc tgaaatggtc accggacggt atgcgtgttg aggtcattca
     9841 ggccggtgag tatgacgacg gggcgcttcc tgcccgggtg caggagattg cacttcaggc
     9901 cgggttagca gagcgcggaa tcagtgcaaa aagcagtaaa gcggcaaaag agaaaaaagc
     9961 cacgaccagt aaagagggct gagtatgctt ctgacaatgg aagagattaa agcccaactc
    10021 cggctggatg aggatttcga tactgatgac cgccatctgc aactgctggc atgtgcggca
    10081 caaaagcgga cggaaacgta tctgaaccgg aagctctatg caccggatga aaccattccg
    10141 gacagcgatc cggacgggct gcacctgccg gatgatattc gtctggggat gctgatgctt
    10201 atcagccatt tttacgaaaa ccgctcgtcg gttacggaag tggagaaact cgacatgccg
    10261 cagagttttg gctggcttgt cggcccgtac aggtactttc cgcaatgaaa attcgtcagg
    10321 cgcagaccag cgcaacctac attctgccgg accccggtga actgaataaa cgcgtcctga
    10381 tccgcctgcg ggtggatatg cccgcggata actttggcgt ggagcctcaa tacccggtta
    10441 cgttccggac atgggcgaag gttatccaga ccagtgccac cacctggcag taaaccgcgc
    10501 agaccgggga cgccatcacc cattacatca ccattcgtta ccgccggggg atcaccgctg
    10561 attatgaggt ggtctgcggt gacagtgtgt accgggtgaa acgtcagcgc gatctgaacg
    10621 gggcgcggcg ctttctgctg ctggagtgta cggagctggg cgaatgtagg cagagtcacg
    10681 gaggcaacaa tgacgacttc ctttttgcac gttgattttc agcagcccgc ggagatgcgc
    10741 tttaaccgcg cccgtgtccg gcgggcgttt gtcacgattg gtcagcgtca tatgcgtgat
    10801 gcccgtcggc tggtgatgcg ccgtgcgcgg tcggcaccgg gtgaaaaccc cggttatcag
    10861 accggacgcc tggctcgttc gattggttac atggtaccca gagccagtaa acatcgccct
    10921 ggttttatgg cacgtatagc ccctaaccag cgtaatggag agggaaaccg ccgtatcacc
    10981 ggtgattttt atccggcttt tttgttctat ggcgtgaggc gaggggcaaa gcgtcgtcgc
    11041 agccatcatc gtggtgcatc cggtggcagc ggctggcgac tggctccacg taataacttc
    11101 atggtggaaa cgcttgaaaa gaaccgcagc tggacacgct attttctggc gcgggaattg
    11161 cgtaaatcac tgaagccgga gcgacgacac agatgaaact gacgcctgtt attgctgcac
    11221 tgcgtgcccg ctgtccgtat tttgaaaacc gggttgcagg cgcggcccag ttcaaaaatc
    11281 tgccggaggt cggaaagctg aaactcccgg cggcatatgt tgtaccgggt gatgattctc
    11341 cgggagaaaa caaaagccag accgactact ggcaggagct gaaagagggt ttctccgtgg
    11401 ttgtcatact gagtaacggg cgtgatgagc gcggtcagtt tgcctcgtat gatgtggtgg
    11461 acgatgtccg gcagatgctc tttaaggctc tgctgggctg gaacccggag gcgtgcggta
    11521 acccgattac ctatgacggc ggcacgctgc tggatctgaa tcgtcatgag ctgatttatc
    11581 agttcgattt ttcggtcatc agcgagctga ctgaagacga tacccgccag caggatgatc
    11641 tgaacagtct ggatgaactg caaacgctgg cgattgatgt tgattatctc gagcccggta
    11701 acgggcctga cggcgatatc gaacatcaca ccgaaataac ccttccttcc tgaggatcct
    11761 catgtttgtc aaacctgtta aagggcggtc agttcctgac cctgcccgcg gcgacctttt
    11821 gcccgccgaa gggcgaaatg ttgacgagaa caactactgg ctgcgccgtg aagcagcggg
    11881 tgatatccgg cgcgtgaata aaaaggtgaa taccgatgac gataagcttt aacaccattc
    11941 cgtcgaatac gctggttccg ttgttttatg cggaaatgga taactcggcg gcgaatactg
    12001 cacaggacag cggagcatcg ctgctgattg gtcatgccaa taacggtgca gagattgttg
    12061 ccaacagtct ggtactaatg ccgtcggcag actatgcacg ccagatttgt ggtgcgggaa
    12121 gtcagctggc gcgtatggtc gaggcttatc gccagaccga cccgtttggt gagctgtatg
    12181 tgattgccgt tccggaagcc acaggcgcgg cggcaacggt tacgctgacg gtgaacgggg
    12241 cggcaaccga aaccggcacg gtgaatgttt atgtgggacg tacccgcgtg caggcaccgg
    12301 tgaccaacgg cgataacgtc acgacgattg ccagcagtat ccaggatgcc atcaatgccg
    12361 ttccggccct gccgtttacg gcctcatctt cggcaggcgt ggtcacactg accgcgcgtc
    12421 ataaggggct ttgcgggaat gaaattcctg tcagcctcaa ttactacggc tttggtgggg
    12481 gcgaagtgct gccagcgggc gtacagattg ccgtggcgac ggggaccgcc ggaacgggcg
    12541 ctccggttct caccggtgcg gtggctgcaa tggcggatga gccgtttgat tatatcggtc
    12601 tgccgttcaa cgacacggcc tccgttaaca cgctggtgac cgagatgaac gataccagcg
    12661 gtcgctggag ctatgcgcgt cagctgtatg gtcatgtgta tacggcaaag atcggcacgc
    12721 tgtcagaact ggtgaccgca ggtgaccagt ttaaccagca gcacatcacc ctggcggggt
    12781 acgaaaaaga gacccagacg cctgccgacg agctggcggc aagccgtacc gcccgcgcag
    12841 cggtgtttat ccgcaacgat ccggcatgtc ccacgcagac cggtgagctg gtgggtatgc
    12901 tgcctgcgcc gaaggggaaa cggttcacga tgaccgaaca gcagaccctg ctgtttcatg
    12961 gcgtggcaac ggcgtatgtc gaaagcgggg tgctgcgcat tcagcgtgat gtcaccacgt
    13021 acaggaaaaa tgcttacggg gttgcggata acagctacct cgacagcgag acgctgcata
    13081 ccagtgcgta tgttctgcgc aaactgaaat ccgtcattac cagtaagtac gggcgtcaca
    13141 agcttgccag cgacggtacc cgctttggtc ccggtcaggc gattgtcacc ccggcggtga
    13201 tcaaagggga actgctggca acctaccgtc agcttgagcg tgcggggatc gtggaaaact
    13261 acgaactgtt taagcagtac ctggttgtgg agcgtgatgc cagcgatccg aaccgcctga
    13321 acacgctgtt cccgcctgac tatgttaacc agttgcgtgt ctttgccgtg gttaaccagt
    13381 tccgtcttca gtattcagag gagtctgcat aatggcccgt atcgggggaa cctgttattt
    13441 caaaatggac ggtcagcagc tatcgctgac cggcggcatt gaggtgccca tgaacaggac
    13501 ggtcaatgat gacatcatcg gcctggacgg ttcagtggac cgcaaggaaa ctcaccgtgc
    13561 gccttatgtc aaagggacct tcaaggtgcc gaagaatttt ccggtgagca aaatcacctc
    13621 gtctgatgag atgaccatca ctgccgagct ggcgaacggt caggtctatg tactgtcgtc
    13681 tgcctggctg cacggcgaag cgaaccataa tgccgaagaa ggcacggtcg atcttgagtt
    13741 ccacggtgaa gaaggggatt accagtaatg aaagagcttg agttaaagaa accgattacc
    13801 gctcatggcg agacactctc cgtactggag tttgatgagc ccaccgggaa ggatgtccgc
    13861 gagctggggt atccctacca gatgaatcag gatgagtccg tcagacttct ggcgcatgtg
    13921 gtgtcgaaat atattgtgcg gctggcgaaa gtgccgcaaa gctctgtcga ccagatgtct
    13981 ccggcagacc tgaatgcagc ggcgtggctt gtggctggtt ttttcctcca ggcctgacgg
    14041 ctgaatacct tactgatcgc ttctttgatt gcgccagcta ctggcgcatt aatcctttcg
    14101 aattgctgaa tatgccgatc agtgaaattc ccttactggt cagtcaggca aacaggatag
    14161 agcaggagaa acgcacacat ggctgaattt gagcttaagg cgttgatcac cggtgtcgac
    14221 aggctttctc ccgcgctgtc gaaaatgcaa aagaaaatcc ggggatttaa acgccaggcg
    14281 gaagaagcgt cacagggtgg gctggcgctt ggtggcggac tggcagcggg tctgacgctt
    14341 tccctgaaat cttatgccga tcaggaaaac gccgccaccg ggctgaaagt cgccatgatg
    14401 gatgcgaacg gcgaggttgg aaagagcttt caggacatca ataaactggc tattggcctg
    14461 ggtaaccagc tacccggtac aacggctgat ttccagaaca tgatgcagat gctggtgcgt
    14521 caggggagcc cggcagaaaa cattcttggc ggtgtgggta aagcgacagc ttatcttgcg
    14581 gtacaactga aaaaaacacc ggaagcggct gctgagtttg ctgcaaagat gcaggatgct
    14641 accggaacgg cctcagaaga catgatgggg ctgttcgaca caattcagaa ggcgttttat
    14701 ctgggcgttg acgacaccaa catgctgtca ttcttcacta aaaccagctc tgttctgaag
    14761 atggtgaata aggacggtct tcaggctgca cagagtcttg cccccatcag tgtcatgatg
    14821 gatcagatgg ggatgaacgg ggagtcggca ggtaatgccc tgcgaaaagt tatccagtcc
    14881 ggattaagcg ttaagaaaat cagggacgtc aataaaatca tggcccgcca gaaactcggg
    14941 gtacagctcg attttactga tggcaaaggg agttttggcg gccttgataa catgttcagg
    15001 caactggcaa agctgcgaaa actgaccgac gttaagcgaa ccggtgtact taaggcaata
    15061 tttggtgatg atgccgaaac ccttcaggtg gtcaatgcgc tgatcgataa aggaaaggat
    15121 ggctacgatc agatccagca gaagatgaat aaacaggcca gcctgaataa acgtgttcag
    15181 gctcagcttg gtacgctgtc caacctgtgg gaggcaatga cggggaccgc aactaacggc
    15241 cttgcggcta ttggcggcgc attttctggt gacgctaaga acatcacgca gtggctgggg
    15301 gagttggggg agaaattcac gaagtttgcg gatgaaaatc cccgggttat tcgcggcgtc
    15361 gtcgggcttg ctgccggtct tgcgattctg atactgggat tgatgggcgt gggcagtgcc
    15421 atcagcattg tcagcaggat catgtcgatg acgccgattg gcatgattgc gacggcgata
    15481 gccatggctg cgggattaat tatcactaac tgggatgttg tcggacctta tttcaagaag
    15541 ctctgggaaa ccattggtcc ttattttgag gctggctggg aactccttaa gaaagttttt
    15601 gcctggtcgc cgctggggat ggtgatcaat cactgggggc cggttgttaa gtggtttcag
    15661 gatatgtggg acaagctgaa gccaattatt gagtggttta ccgacagttc cggagacacg
    15721 gtcgatgcca ttaactctgc gcagtggggc gcgggtgctt atgatgctta tgggacggga
    15781 ataccggcac ggggatacac accttatccg gtggtggatc tggctcagtc aaacaacgcc
    15841 tccggtgcca taggcccgaa tcccttcatg attaataaag ctactgcgcc aaaagttgat
    15901 ggtgagatca aggtctcttt tgtgaattcg cctccgggta tgcgggttat ggaaacgcga
    15961 tccagcggtt ttgatgtcag ccatgatgtt ggctatacgc gctttggcag gtaatgaaaa
    16021 attaatctgt taatgagtcc cacaccggtg ggatttttta tgtacggagt ttatatgacg
    16081 tggaaagaca gacttcagga cgcgtcattt cgcggtgtgc cgtttaaggt tgaagaagaa
    16141 agtgcgggaa ccggtcgtcg tgtggaaacg cacgaatacc cgaaccgcga caaaccctat
    16201 accgaagacc tggggaaaat cactttccgc ccgtccatca cggcttatgt ggtgggcgat
    16261 gactgctttg accagcgcga tcgcctgatt gacgcgctga ataaacccgg tcccggcacg
    16321 cttgtccatc cgacatacgg cgagctgaaa gtctgtgttg acggagaagt tcgggtcagc
    16381 acatcgaaga gtgaagggcg tattgtccgc tttgacctga agtttgtcga agcgggagaa
    16441 ctctcttacc ccacatcagg tgcggcgacg gcgcagacgc tgatgtcatc ctgttctgca
    16501 ctggatgact gcatcagtga cagcttcagc ggtttcagta tcgatggtgt ggcggatttc
    16561 gtgcagaacg acgttatcgg taatgccagc ataatgctgg ggtatgtttc tgatgcgatg
    16621 aaagtggtgg attctgccgt atcggatgcc gccaggctgt tgcaggggga tatctcggta
    16681 cttctgccgc cgccatcgtc aggcaaaaat ttcgttgagc aggtgcagaa aatgtggcgt
    16741 accgggaaac gcctttatgg taacgccagc gacctggtca ccatgatcaa aacgctttcc
    16801 gatgtcagcc tcggcagcga tctgcaaccg cgcggcgtct ggaaaacgga cagtaaaacc
    16861 accgccacgg cgacgcagca gcgtaacgtg gttgccagca cccttcgtac gaccgcaatc
    16921 agcgaagcgg cgtatgccgt tacccgattg cctgcgccaa caacttccgc ggtgatgcag
    16981 aatgccgcag tggggcaggc aacaacaccc gcgcagagca cttgctggcc ttccgccacg
    17041 catccggcac tgaacaatgc accggcggtg aaaaacacgg ttgacctgcc gacgtgggaa
    17101 gaactgactg acattcgcga cacactgaat acggcaattg ataaggagtt gtcccgtaca
    17161 accagcgatg cgctgtttct ggcgctgcgc cgggtgaaag cagatctgaa tgcggatatc
    17221 aacacgcgcc ttgaacagtc tgcacggatc attcagcgca caccggatga ggttttaccc
    17281 gcgctggtgc tggcggagac ctggtttgat aacgcggcgc gtgacgcgga cattatccgg
    17341 cgtaatgcca ttacgcatcc cggctttgtg ccggtgatcc ctctgaaggt gccagtgcaa
    17401 tgaacgacaa tgtcacgcta cgggtaaatg gccgggagtg gaatggctgg acattggtgc
    17461 gcatcggtgc cggtattgaa cggctggcgc gggatttcag tgtggagatc acccgccagt
    17521 ggccgggaga tgagggtatt accacgcttc agccgcgcat taaaaacggt tcaaaagtgg
    17581 aggtgctgat tggtgatgag ctggtgatca ccggctgggt ggaggcgacg accgttcgtt
    17641 acgatgcccg ttcggtcatc accggtattg ccggacgcag tctgaccgct gacctgattg
    17701 actgtgcagc cgaaccgaca cagtttaacg gacgatcgct ggtacagatt gcgcaggcgc
    17761 ttgctgcgcc tttcggcatt gaggtggtga acagcggtgc gccgtcgggt gttattcctg
    17821 atgttcagcc tgatcacggt gaaacggtga ttgaggtaat caacaaaata ctcggtcagc
    17881 agcaggcact ggcttacgac gacccgcacg gcaggctggt gattggcggt attggctcaa
    17941 cgcgggcaca tactgcgctg gtactcgggg aaaacatcct ttcctgcgat acggagaaga
    18001 gtatccggga gcgattttct gtttaccagg tggcggggca gcgtgccgga aatgacgatg
    18061 atttcggtga ggccaccacc accgcgctgc gggcccgtac agaggacgca tttattgccc
    18121 gttaccgtcc gatgtatatc aggcagacag ggcaggctac gggggcaggc tgtattgcgc
    18181 gtgctgactt tgaagcccgg caacgggcgg cgcggacgga tgaaaccacc tatgtggtgc
    18241 agggctggcg acagggtaac ggtacgctgt ggcagcccaa ccagcgggtg attgtctttg
    18301 atccggtctg tggtttcgac aataccgaac tgcttgtttc ggaagtcacg tttactcagg
    18361 accagaacgg caccctgacg gaaatccgtg tcggcccgcc tgatgcttat ctgcctgaac
    18421 ccgaagcccc cggcgcgcgg aaaaagaaaa aagccagagt acaggaggac ccgttctgat
    18481 gaggacgatt gaagccatgc agcgacaact cctcggcctg attgggcggg cagtggtgaa
    18541 aagcatcagc gccgccacga aatgtcagac cgtggatgtg tccctgattg ccggtgaacc
    18601 caaagccggg attgaacatc ttgaacccta cggttttacc gcaagggcaa acagcggtgc
    18661 ggaagcggtg gtgttgtttc cggatggcga ccgttctcat gcggtggttg ttacggtgtc
    18721 ggaccggcgc taccgcctga aagggctgca gacgggtgag gtggctgtct atgacgatca
    18781 ggggcagtct gtgacgctga cccgggaggg gatcgtggtg gacggtgcag gtaaaacgat
    18841 cacgtttcgc aatgcgccta aggcacgttt tgaaatggac ctggaagtga caggacaggt
    18901 gaaagacctg tgcgactccg gcggcaccac catgtcagcg atgcggcttg cctataacgg
    18961 gcatcgtcac agagagaacg gtcagggcag taacaccgac aaacctgata aagcgatggg
    19021 ggcatgatgg aactgtggct gacggtgaac ggtaaacgca cctgcgccag cgcaccgctg
    19081 gatccgctga cccgcgccgt ggtgatttcc ctgtttacct ggcggcgggc ggagcctgat
    19141 gacaacgccg acgtcccgat gggatggtgg ggggatacct ggcctgcggt acagaatgac
    19201 cgttacggct cccgactgtg gctgcttcag cgcagcaaac tgaccaatca gctggtgcag
    19261 acggtaaggg ggtatatccg cgaatgcctg caatggatga ttgatgacgg cgtggtgtcc
    19321 cgtattgatc tggatatccg ccgcaccggg attaatgaac tgggtaacag tatcactctc
    19381 tggcgtcgtg acggaccggt aatgatttct tttgatgatc tgtggagtgc gataacgcat
    19441 ggcggacagt gaatttcagc gcccgacgct ggcagaaaat atcagtatgc tccgtaacga
    19501 tttattcgtc aggctggacg tcagcgacac gctccggcgc atggatgaag acgtgcgggc
    19561 aaaggtgtat gcggcggcgc tgcatacggt ctacgggtac atcgattatc tggcaatgaa
    19621 tatgctgcct gacctgtgcg atgagtcctg gctggcgcga catgctgcga tgaaacggtg
    19681 tccgcgcaag ggggccacgg ctgccagcgg gtatatgcgc ttggaaggtg tcagcgatgg
    19741 cctgaaggtg actgccggga gcgtgattca gcgcgatgac ctggttcagt acacggcaac
    19801 tgccgatgca accagctccg gtggtgtcct gcgtgtgccg atcacttgct caactacagg
    19861 cgcggtcggt aacgctgacg acggtacggc attaatcctg gtcacgccgg tgaatggtct
    19921 gccgtcttcc ggtgttgcag ataccctgac tggcggattc gatactgaag atctggaaac
    19981 gtggcgcgcc cgggtcattg agcggtatta ctggacgccg cagggcgggg ctgacgggga
    20041 ctatgtcgtc tgggctaaag aagtgcccgg cattacccgt gcatggacat accgtcactg
    20101 gatgggaacg ggaactgttg gtgtgatgat tgccagcagt gacctgatta accccattcc
    20161 ggaagaatca acggaaacgg cggcaagaca acatatcggg ccactggccc cggtggcagg
    20221 ctctgatttg tatgtgttca ggccggtggc acatacggtg gattttcata ttcgcgtgac
    20281 gccggacaca ccggaaatac gggctgccat caccgcggag ttgcgttcgt tcctgctgcg
    20341 tgatggttat ctgcagggag aactgaaggt atcgcgtatc agtgaggcga tttccggtgc
    20401 gaacggggaa tacagccatc agttgcttgc accggcagac aatatctcca ttgcaaaaaa
    20461 tgaactggcg gttctgggga cgatttcatg gacgtgacaa acgatgatta catccgtctg
    20521 ttgtcggcac tgctgccgcc cggtccggcg tggtcagcca gcgatccggc gattgccggt
    20581 gcggcacagt cattaacccg cgttcatcag cgtgcggatg ccctgatgcg ggagctggat
    20641 ccgcgcacca ccaccgaact gataaatcgc tgggagcgtc tgtgcggcct gccggatgaa
    20701 tgtattcccg caggaacaca gacccttcgc cagcgtcagc aacgactgga tgcgaaggtt
    20761 aacttggcgg gcggcatcaa tgaggatttt taccttgcac agcttgctgc cctgggcaga
    20821 caagacgcca ccatcacgcg atacgacaaa agcacgttca cctgctcatc ggcctgtact
    20881 gacgcggtga atgcgccgga atggcggtat tactggcagg tcaacatgcc agccgccacc
    20941 aacagcacct ggatgacatg tggcgatccc tgtgattccg cgctgcgtat ctggggcgac
    21001 accgtcgtcg aatgtgtgct taacaaactc tgcccttcgc atacctacgt aatttttaaa
    21061 tatccggagt aatccatgca tcgtatagac acgaaaaccg cgcagaagga taagttcggc
    21121 gcgggtaaga acggttttac ccgtggtaac ccccagactg gcacgcttgc caccgatctg
    21181 gatgatgact actttgacat gttgcaggag gagctttgca gcgtggtgga ggcatccggt
    21241 gccagcctgg agaaggggcg gcacgatcag ttgcttaccg cacttcgcgc gctgctgtta
    21301 agccgcaaga atccgtttgg cgatatcaaa tcggatggca cggtgaaaac ggctctcgaa
    21361 aaccttggtt tgggagaagc agctaaaagg aatgtaggta caggggccga a
//
LOCUS       DADLSA010000056        21395 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.056, whole genome shotgun sequence.
ACCESSION   DADLSA010000056 DADLSA010000000
VERSION     DADLSA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.056"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            59..931
                     /locus_tag="JN691_002143"
     CDS             59..931
                     /locus_tag="JN691_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HAY7243590.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(991..2289)
                     /gene="eno"
                     /locus_tag="JN691_002144"
     CDS             complement(991..2289)
                     /gene="eno"
                     /locus_tag="JN691_002144"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAY7243591.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2377..4014)
                     /gene="pyrG"
                     /locus_tag="JN691_002145"
     CDS             complement(2377..4014)
                     /gene="pyrG"
                     /locus_tag="JN691_002145"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAY7243592.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4242..5033)
                     /gene="mazG"
                     /locus_tag="JN691_002146"
                     /pseudo
     CDS             complement(4242..5033)
                     /gene="mazG"
                     /locus_tag="JN691_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
     gene            complement(5129..7363)
                     /locus_tag="JN691_002147"
     CDS             complement(5129..7363)
                     /locus_tag="JN691_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAY7243593.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7411..8712)
                     /gene="rlmD"
                     /locus_tag="JN691_002148"
     CDS             complement(7411..8712)
                     /gene="rlmD"
                     /locus_tag="JN691_002148"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAY7243594.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLGALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLQEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8769..11525
                     /gene="barA"
                     /locus_tag="JN691_002149"
     CDS             8769..11525
                     /gene="barA"
                     /locus_tag="JN691_002149"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAY7243595.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11757..13097)
                     /gene="gudD"
                     /locus_tag="JN691_002150"
     CDS             complement(11757..13097)
                     /gene="gudD"
                     /locus_tag="JN691_002150"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY7243596.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13118..14458)
                     /locus_tag="JN691_002151"
     CDS             complement(13118..14458)
                     /locus_tag="JN691_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY7243597.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14460..15812)
                     /locus_tag="JN691_002152"
     CDS             complement(14460..15812)
                     /locus_tag="JN691_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7243598.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKSVAGQ
                     "
     gene            complement(16246..16695)
                     /locus_tag="JN691_002153"
     CDS             complement(16246..16695)
                     /locus_tag="JN691_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAY7243599.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16713..17495)
                     /gene="truC"
                     /locus_tag="JN691_002154"
     CDS             complement(16713..17495)
                     /gene="truC"
                     /locus_tag="JN691_002154"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAY7243600.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17495..17824)
                     /locus_tag="JN691_002155"
     CDS             complement(17495..17824)
                     /locus_tag="JN691_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAY7243601.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18446..18991)
                     /gene="syd"
                     /locus_tag="JN691_002156"
     CDS             complement(18446..18991)
                     /gene="syd"
                     /locus_tag="JN691_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAY7243602.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19059..19907
                     /gene="queF"
                     /locus_tag="JN691_002157"
     CDS             19059..19907
                     /gene="queF"
                     /locus_tag="JN691_002157"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAY7243603.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20019..21383
                     /gene="ppnN"
                     /locus_tag="JN691_002158"
     CDS             20019..21383
                     /gene="ppnN"
                     /locus_tag="JN691_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAY7243604.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5257 a         5701 c         5104 g         5333 t
ORIGIN      
        1 tctaaaatta ccgatgccgc cgtcaattta tttatccaga tttaatgaga aatttataat
       61 gcgctatttc atactaatgt tcactttcgt atgttccttt gttgcagccc aaccaactat
      121 tgtcccacaa ttacagcaac aagttacggg tcttacgagt agcttaaact cacaagaaaa
      181 gaaagaactg acgcacaagt tagaatctat tttcaataat acccaagtgc aaattgctgt
      241 attaattgtt tccacaacca aagatgagac aattgaacaa tatgctacaa gagtttttga
      301 caattggcgt ttaggagatg ccaaacgtaa tgatgggata ctgatcgttg ttgcctggtc
      361 ggatcgcact gtccgcatcc aggtaggtta tgggctggaa gaaaaggtaa ccgatgctct
      421 ggctggagat atcatccgta gcaacatgat acccgcattt aaacaacaaa aattagctaa
      481 gggattagag ttagctataa tcgctttgaa taaccaactc acttcacaac accaatatcc
      541 ggctaatcct tcagaaagtg aatcaacgtc ttccactgat cattattact ttgctatttt
      601 ttgggtattt gcagtgatgt tcttcccttt ctggtttttt catcaaggca gtaatttttg
      661 tcgcgcatgt aaaagtggag tctgtatttc agcaatccat cttttagatt tattcctgtt
      721 ctccgataaa actttttcca ttgctgtatt ttccttcttt tttactttca ccatatttat
      781 ggtctttacc tgtttatgtg tacttcagaa aagagcatct ggtagaagtt atcattcaga
      841 caacagcggt tccgctggag gctcaagtgg cggcggattt agcggcgggg gcggttcttc
      901 tggcggtggc ggcgcatccg gccgctggta actccaccat aaaaaatgcc agcccgaagg
      961 ctggcattat caaatcagat aaagtcagac ttatgcctgg cctttgatct ctttacgacc
     1021 gttgtacggt gctttttcgc ccagagcttc ttcgatacga atcagctggt tgtatttagc
     1081 aacacggtca gaacggctca tagaaccagt tttgatctgg cctgcagcag taccaacagc
     1141 cagatcagcg atggtagcat cttcagtttc accagaacgg tgagagataa ctgcagtgta
     1201 gccagcatct ttcgccatct tgattgcagc cagagtttcg gtcagagaac cgatctggtt
     1261 gaatttgatc aggatggagt tagcgatacc tttttcgata ccttctttca ggatcttggt
     1321 gttggttacg aacaggtcgt caccaaccag ctggattttg tcgcccagaa ctttggtctg
     1381 gtatgcgaaa ccgtcccagt cagattcgtc cagaccgtct tcgatagaaa cgatcgggta
     1441 ctgtttggtc agttcttcca ggaagtgagt gaattcttca gaggtgaacg ctttgttgcc
     1501 ttcgccagcc agaacatatt taccatcttt gtagaattca gaagctgcgc agtccatcgc
     1561 cagagtgatg tctttgccca gttcataacc agcagcttta acagcttcag cgataacagc
     1621 cagagcttca gcgttggaac ccaggttcgg cgcatagcca ccttcgtcac caacagcagt
     1681 gttcatgcct ttcgctttca gaacttttgc caggtgatgg aaaacttcag aacccatgcg
     1741 gatggcttct ttcacagttt tcgcgccaac cggctgaatc atgaattcct ggatatcaac
     1801 gttgttgtca gcgtgctcac caccgttgat gatgttcatc atcggaaccg gcatggagta
     1861 tttgcccgga gtgccgttca gttcagcgat gtgctcgtac agcggcatac ctttagcagc
     1921 tgcagcagct ttggcgttag ccagagatac agccaggatt gcgttcgcgc cgaatttgga
     1981 tttgttttcg gtgccgtcca ggtcgatcat gatcttgtca atgccagcct gatctttagc
     2041 atctttgcca atcagcgcct gagcgatcgg gccgtttacc gcagcaacag ctttggttac
     2101 gcctttaccc aggaaacggg atttgtcgcc atcgcgcagt tccagagctt cacgggaacc
     2161 agtagaagca cctgacggag cagctgccat accgacgaaa ccaccctcca gatgtacttc
     2221 ggcttcaaca gtcgggttac cacgggagtc gatgatttca cgaccgatga tttttacgat
     2281 tttggacatt aggttttcct caagtcacta gttaaactga aactccagac aaacaacgcg
     2341 tacccagggt acgcgttgcc gttctaactt ttttacttac ttcgcctgac gtttctggaa
     2401 ctcgctggcg gctttcacaa agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt
     2461 aaactccgga tggaactggc aagccacgaa ccacgggtga ttcggaactt cgatgatctc
     2521 gaccaactga tcatccccgg aacggcccgc aacgcgcaga cctgcatctt caatctgttt
     2581 caacagcatg ttgttgactt cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt
     2641 gtatagctgg cgaaccaggc tatcgtcaac caactggcac tgctgtgcgc cgagacgcat
     2701 ggtaccgccg agatcgctct tctcgctacg aacttcaacg ttgccgtttt catcgcgcca
     2761 ctcggtaatc agcgccacaa ccgggtactt acagtctggc acaaattctg tagagttggc
     2821 gttctccatg ttggcaacat ggcgagcgta atcaattaac gccacctgca tacccaggca
     2881 aatgcccaga taaggaatat tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc
     2941 tacgccacga tagccgaaac cgccaggtac gaggattgcg tccagacctt taaggatttc
     3001 aacgccgcgc gtttcaacat cttgtgaatc gatcagtttg atgttgacgc tgacacgatt
     3061 cttcagccca ccgtgtttca gtgcttcgat cactgattta taagcatccg gcagttcaat
     3121 gtacttgccg accataccga tggtgacttc acttaccgga ttcgcttctt cgaagataac
     3181 ctgttcccat tcggacagat ttgcttccgg gcagtttaag ctgaatcgtt tacaaatata
     3241 atcgtccagc ccctgagatt tcaacaggcc cggaatttta tagatggaat cgacgtcttt
     3301 cagagaaata accgcttttt ccggaacatt acagaacaat gcaatcttcg cacgttcgtt
     3361 cgccggtaca gcgcgatctg aacgacaaat caggatgtca ggctggatac cgatggagag
     3421 cagctctttt acagagtgct gagtcggttt ggttttgact tcaccagacg ctgccatgta
     3481 cggcaccagc gtcaggtgca taaacagagt gtgctcacgg ccaatttcaa cagccatctg
     3541 gcgaatagct tcgaggaacg gcaaggattc gatatcacct actgtaccgc cgatttctac
     3601 cagtactacg tcatgacctt cgccaccttc cagcacgcgc tctttgattg cgttagtgat
     3661 gtgcggaata acctgcacgg ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac
     3721 gtcagagtag atacgacccg tggtgaagtt gttgcggcgg ctcattttgg tacgaatgaa
     3781 acgctcgtag tgccccaggt ccaggtcggt ttcagcgccg tcttcagtaa cgaacacttc
     3841 cccgtgttgg attgggctca tagtacctgg atcgacgttg atgtaaggat ccagtttcat
     3901 gatggtcaca ttgaggccac gggcttcaag aatggctgcg agggaggctg cggcaatgcc
     3961 tttacccaga gaggatacga ccccgccggt cacaaaaata tagttcgttg tcatgctgaa
     4021 cctgagaagt taggttgaaa agacgatgga ataaccaaga cgggaaagca gtatacccga
     4081 acatgacctg tgccacaaac tttcattatc cctcctcttc gccagcgcac tattgaaatc
     4141 aggagtgaga aaatagcccc tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca
     4201 tttaaaaaat gacacaaatg gcgcttgacc gcgtaattcc cttagagatc aatttcctgc
     4261 cgttttacct gttgccagac ttcttccatt gtttcgaggt caacacctgt catttccagt
     4321 ccacgcgcgg caacaatacg ctccacttcg cgaaaacgac gctcgaattt ttcgttcgct
     4381 ttttgcaatg cgatttctgc tttcgtccct aaatggcgag ccagattaac cgtggcaaac
     4441 agcaggtccc ccatttcctc ctccagttta gcctggtcga caacagcctg ccgcgcctcg
     4501 tacatcacct cgtcgatctc ttcgtagact ttatcaacca ccgggccaag cgtcgtccag
     4561 tcgaagccaa cgttggcgca acgtttctgg attttttgcg cccgcattaa agccggtaaa
     4621 ctacgaggaa tatcgtccag cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg
     4681 atttgctccc aacgggcaag cacttcacta ctgttttcgg cagaactatc agcaaaaaca
     4741 tgcggatggc gacgctctaa tttatcgcta atagcagcgc aaatatcatt aaagtcaaag
     4801 cgcccttctt cctgagccat ttgcgcgtaa aacaccacct agaatagcag atcgcccagt
     4861 tcaccgcgaa gatcgtcaaa atcttcacgg gcgatggcgt ccagcacttc gtaggtttct
     4921 tcaagggtgt aaggcgcaat ggtggcaaat gtctgctctt tatcccacgg gcagccgttt
     4981 tccggatcgc gcaggcgctg cataatagtg agcaaacggt cgatttgatt cattgaattg
     5041 tcctgaaaat tgcgggtctg tcaggtgtaa taccttcgag taaaagatat cgctggatac
     5101 ggctgacgcc atatccagct atgtaagact aactcccgtg caaccgacgc gcgtcgataa
     5161 catccggcac ctggttgagt ttacccagca cgcgccccag cacttgcagg ttgtaaatct
     5221 caatggtcat gtcgatggtc gccagttgct gtttggtgtc gctacggctg gcaacgccaa
     5281 gcacgttcac cttctcgttg gcgagaatgg tcgtgatatc acgtaacaac ccactacgat
     5341 cattagccac cacgcggacc accagcgaat atccggcgga gtagctctca ccccataccg
     5401 cgtcaacaat gcgttctggc gcatgggagc gcagttccgc cagttgttcg caatcggcgc
     5461 ggtgtactga aataccgcgt ccctgggtaa tgaagccgac aatctcatct ccaggaatcg
     5521 gctggcagca gcgcgcgatg tggtgcatca ggttaccaac accttcaacc accacacgac
     5581 cgttatcttt actgcggttt tgcggtgtgt agcttttttg ctgaagctgt ttcagcgcgg
     5641 cggcgtcctg ctcttcggca ctcggcttat taaattgcga ttgcaggaag ttcaccatct
     5701 gattgagacg gatatccccg ccaccaatcg ccgccagcaa ctcgtcgaca tcattgaagt
     5761 tgtaacgcgg cagcagatgt ttttctgctt ctttcaggct gatccccaga tgttccagct
     5821 cgtcgtcaag gatttgccgc ccagccagaa tgtttttgtc acggtcctgt ttacggaacc
     5881 aggcgtgaat tttcgaacgc ccacggctgg ttgtgacgta accgaggttt gggtttaacc
     5941 agtcacggct ggggttcggc tgtttctggg tgataatttc aatctggtcg cccatttgca
     6001 gctggtaggt gaacggcaca atgcgcccgc caatttttgc cccgatgcag cggtgtccga
     6061 catcactgtg gatgtggtaa gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga
     6121 catcaccttt cggcgtaaag acatacaccc ggtcgtcaaa cacctgacta cgtacttcgt
     6181 cgagcatttc gccggaatca gccatctctt cctgccacgc aatcagttta cgcagccagg
     6241 caatccggtc ttcatgtccc gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc
     6301 agtgcgcagc aacacccaac tccgcatctt catgcatctg tttggtgcgg atttggatct
     6361 caacggtttt tccacccggc cccagaacca cggtatgaat agactgataa ccgtttggtt
     6421 tcgggttagc gacgtaatcg tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta
     6481 tccccagtgc ggcataacaa tcctgtaaac gctcggcgac aatacgtacc gcacgcacat
     6541 caaacagctc atcgaaggcg aggttctttt tctgcatttt gcgccagatg ctgtagatgt
     6601 gtttcggacg accatacact tcagctttaa cgccttcagc tttcatctca gcgcgcagat
     6661 ggccgacaaa ctcttcgata tagtgttcgc ggtcgagacg gcgttcatgc agcagttttg
     6721 cgatgcgttt gtattcggtc gggtggagat aacggaagca gtaatcttcc agctcccatt
     6781 tcagttgccc aatcccaaga cggttcgcca acggcgcgta gatattggtg cactcttttg
     6841 ccgccagtac acgttcatct tccggcgcat cttttacttc gcgcagatga gcaatacgct
     6901 ccgccagttt gatgactacg cagcgaaaat catcgaccat cgccaataac atccggcgaa
     6961 cgttatcgac ctgttcggag gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg
     7021 ccgccatatc acgcacgccg tgaataaggt taacaaccga cttaccgacg ctctcacgca
     7081 gcacatcttc gctgactacg ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg
     7141 tgtcaatgtc catacttaat gtcgagagga tctccaccat ctcaacacca cgccacaata
     7201 acagactggc atccggatgc ccctgcgtct gttgcagaca atacgcccag gtttcggcta
     7261 agcactcaca cgacttctgg ctggtaatac ccagacttgc gatccatttt tccggatcaa
     7321 attcaccagc cttattgata tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg
     7381 accagacctg ccgaaatcgg caaatcgtaa ctatttaacg cgcgagaaaa gtaccatcga
     7441 ttccagatgt cccgtgtgtg ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc
     7501 tgcttttaat aacgcttcgc tatcccgagc cagcgttgca gggttacagg atacataaac
     7561 tatacgaata ggttccagtt ttataatttg ctgcataaca cctgcggcac ctgctcgcgc
     7621 cgggtccagc aacactttat cgaagccgtt tttcgcccac ggctgctttg tgacatcttc
     7681 ttgaagattt tcgtgataaa acgtcacatt ctgtaagcca ttaagacgcg cattctgctg
     7741 gcctttttcc accagcgccg gaacaccttc tacaccgacc acactggcag cttgtgtcgc
     7801 caatggcagt gtaaagttgc ccataccgca gaacagatcc agtacgcgat cttcaggttg
     7861 cacatccagc cattccaacg cacgcgctac cattttttgg ttcacacccg cattgacctg
     7921 aataaaatcg cgcgggctaa aagttaagcg caacccgttt gagtcatacc agggcatctc
     7981 accagagacg gtttcgagta tctcactatc gggggcgaga tacagatcca ggccttcaga
     8041 atgcgaaaag cgttccagtt tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa
     8101 aatcatcagc gtgccgctgg ttgcctgtac cagttcaaca tgaccaagat ggcgcatagc
     8161 ttgtaagctg cccagacatg ccctgacttt gggcagcaat gctccaagtt ggggcgctaa
     8221 aatggggcat tgtttgacgt cgacaatgtc actggagccc gctttgcgaa accccatctg
     8281 aagttgctgt gttttcggta agtagttcag acttaaacgc gcgcggcggc gatagcccca
     8341 gggaacatcg gcgatcactt cagagacatc gtgtttcatt aatcgggcga gtgccgcact
     8401 tttgcttcgc tgctgtaaat ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc
     8461 aaaatgagga cagcgtggcg tttcgcgttc cgggctatcg cttaaccggc gtacgacttt
     8521 agcgcgggca tactgttttt tatcttcagt aacagcaact tccgcgtttt cctgcggcaa
     8581 taatccgggg ataaataacg ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc
     8641 gaggtcgttg actgaaacgg ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta
     8701 gaattgcgcc attggcgaga ctttctcaat ttaacagtgt gaccttaatt gtcccataac
     8761 ggaactccat gaccaactac agcctgcgcg cacgcatgat gattctgatc ctggcaccga
     8821 ccgtccttat tggtttattg ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc
     8881 agcgtcaact ggaagatgcc ggtgctagca ttattgagcc gcttgcagtt tctactgaat
     8941 atggcatgag cctgcaaaat cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc
     9001 gccattccga tattgttcgc gcgatttcgg tttatgatga aaataaccga ctctttgtca
     9061 cctccaattt tcatcttgat ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc
     9121 gccagctcac tgtcactcgt gacggcgata ttatgatcct ccgcacgccg attatttctg
     9181 agagttactc ccccgacgaa tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg
     9241 gatatattgc gcttgagctg gatcttaaat cggttcgctt gcagcaatat aaagagatct
     9301 ttatttcctg cgtgatgatg ctgttttgta tcggtattgc gcttattttt ggctggcgct
     9361 taatgcgcga tgtaaccggt ccgattcgca acatggtgaa taccgtcgac cgcatccgtc
     9421 gcgggcaact cgacagccgg gtggaaggat ttatgctcgg cgagctggat atgctgaaaa
     9481 acggtatcaa ctcgatggca atgtcgctgg ctgcttatca cgaagagatg cagcacaata
     9541 tcgaccaagc gacgtccgat ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg
     9601 agttggatct ggcgaaaaag cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg
     9661 caaatatgtc acacgagctg cgtacaccac tgaatggtgt tattggcttt acccgcctga
     9721 cgctgaaaac agaattaaca ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg
     9781 caaataattt gctggcaatt attaatgatg ttctcgactt ctcgaaactg gaagcaggta
     9841 agctgattct ggaaagtatt ccattcccac tacgcagcac gctggatgaa gtcgttactc
     9901 tgctggcaca ttcttctcac gataaagggt tggaactgac gctcaatatt aaaagcgacg
     9961 tgcctgataa cgtgatcggc gacccactgc gattacagca aatcatcact aacctagtgg
    10021 ggaatgcaat taaattcacc gagaatggca acattgatat tctggtagaa aaacgtgcgc
    10081 tgagtaatac caaagtgcag attgaagtgc agattcggga taccggcatt ggtattcctg
    10141 aacgcgatca atcgcgctta ttccaggcct tccgacaggc tgatgccagt atttcccgcc
    10201 gtcatggtgg caccgggctg gggctggtga ttacacaaaa actggttaat gaaatgggcg
    10261 gcgatatttc gttccatagc cagccgaatc gcggttcaac tttctggttc cacattaatc
    10321 tcgatctgaa cccgaacatt attatcgaag ggccatccat ccagtgcctc gcaggtaagc
    10381 gcctggccta tgtcgaacca aactccgcag cagcgcaatg cacgctggat attttaagtg
    10441 aaacgccgct ggaagtggtt tatagcccaa cgttctccgc gctgcctccc gcgcattacg
    10501 acatgatgtt gttaggcatc gcggtgacct tccgcgagcc gctaacaatg caacatgagc
    10561 gattagcgaa agcggtatcg atgaccgatt tcctgatgct ggcacttcct tgccatgcac
    10621 aagtcaatgc tgaaaaactc aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa
    10681 cacctacgcg cctgttgcct gccctgacgg aattttgtca tcacaaacaa aacacgcttt
    10741 tgcctgtaac cgatgaaagt aagctggcaa tgacagtcat ggcggttgat gacaaccccg
    10801 ctaacctgaa acttatcggc gcattgctgg aagatatggt gcaacatgtg gaactttgcg
    10861 atagcgggca tcaggcagtt gaacgggcga aacagatgcc gttcgatttg atcttaatgg
    10921 atattcaaat gccggacatg gatggcattc gggcctgcga actcatccac caactcccgc
    10981 atcagcgaca aacgccggtt atcgcggtaa cggcgcatgc aatggccggg caaaaagaga
    11041 agctgcttgg cgcagggatg agcgattatc tggcgaaacc gattgaagaa gagcgattgc
    11101 ataatttgtt gttgcgctac aagcctggca gcggtatttc gtctcgcgtc gtgacgcccg
    11161 aagtcaacga aattgtggtg aacccgaatg cgaccctcga ctggcaactg gcactacgcc
    11221 aggcagcagg aaaaaccgat ttagcgcgcg atatgctgca aatgttactc gatttcctgc
    11281 ctgaagttcg caacaaagtt gaggaacagc tggttggaga aaacccggaa ggcctggttg
    11341 atttgattca taaactgcat ggcagttgcg gctatagcgg cgtaccacgt atgaagaatc
    11401 tctgccagct gatcgaacaa cagctacgta gcggtactaa agaagaagat ttggagccgg
    11461 agctgctgga actgttggac gagatggata atgtcgcgcg cgaagccagc aaaattctcg
    11521 ggtaacggac gtcttgtcga gtagcaatcg gctttttatg acgctggttt cagattggtg
    11581 acaaagtgcc tgtgtttatg ccggatgcgg cgttaacgcc ttatcctgcc tacaaaacca
    11641 attaaattca atgaattaaa aaatgatgta gacctgatac atagcacaac aggctatttt
    11701 gcgtttagca tcagtctcaa accggctcca gatagagccg gttttggttt tctgtcttaa
    11761 cgcaccatgc acgggcgctt gttatcgaac gtccagccag gaatcagata ctgcattccc
    11821 atcgcatcgt cacgcgcgcc aagcccgtgt ttctgataca gctcatgggc tttcatcact
    11881 tgatccatat cgatttctac acccagcccc ggtttttctg gcacctgtac cagcccgcct
    11941 ttgatctcaa acggttcttt ggtcaggcgc tgattacctt cctgccagat ccagtgcgta
    12001 tcaatagcag taattttacc cggtgcagcg gcggcaacat gggtaaacat cgccagggaa
    12061 atatcgaagt ggttgtttga gtgtgaaccc caggtcaggc caaattcatg gcacatttgc
    12121 gccacacgta ccgaaccttg catagtccag aaatgcggat ccgccagcgg gatatcaacg
    12181 gattgtaacg agagcgtatg gcccatctgc cgccagtcgg tggcgatcat attggttgca
    12241 gtcggcaggc cagtcgcgcg acggaactca gccatcactt cacgcccgga gaaaccttgc
    12301 tccgcaccac acggatcttc tgcataagcc agcgaacctt tcaggtattt accaatttta
    12361 attgcttcgt tcagcgacca ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag
    12421 cgttgcgcca gtgccacaat agactcggct tcttcttccc cggccagtac accgcctttc
    12481 agtttgaaat cgttgaagcc atatttttca tacgccgctt ccgccaggcg caccaccgca
    12541 tccggcgtca tcgcttcttc atgacgcagg cgataccagt cgcatgagtc atccggctgg
    12601 ctttgatacg gcagaggtgt ggctttgcga ttaccgacga agaacagata accgagcatt
    12661 tcgacttcgc tacgctgttg accatcgccc agcagcgatg ccacgtttac ccccagatgc
    12721 tgccccagca gatccagcat tgccgcttct atcccggtaa ctacatgaat agtggtacgc
    12781 aaatcaaatg tctgcaaacc gcgcccacta gcatcacgat cggcaaaagt attacgcacc
    12841 agcgtcagaa cgtttttgta ttcacccagc gttttaccta ccaccagcgg aatcgcatct
    12901 tccagcgttt tacggatttt ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa
    12961 ttatctttga taatcacaat attacgcgtg aagaacggtg cgtgtgcacc actcagattc
    13021 atcagcatac tgtcatgacc cgccaccggg atgacctgca tttcagtaac aacaggcgtc
    13081 gtaaattgag aactcatatt tatgtccttt ttcagaatca atgacggccg aaaacgggac
    13141 gtttacggtc aaaggtccag ccggggatca ggtactgcat cggacctgcg tcgttacgcg
    13201 caccgcaagg cagacgttta taggcctcat gtgccttttg tacctgttcc cagtccagtt
    13261 ccacgcccag accgggcgca tcaggaacgg caatttttcc gtttttaatc tccagcggat
    13321 ttttggtcag gcgacaatcg ccctcctgcc aaatccagtg ggtatcgata gcggtaggat
    13381 tacccggtgc cgccgcgccc acatgggtaa acatcgccag agagatatcg aaatggttgt
    13441 tggaatggca gccccaggtc agcccccagt cgtcgcaaag ctgcgccacg cggactgcac
    13501 cggaaagcgt ccagaagtgc ggatcggcaa gtggaatatc taccgcattg agcatcaccg
    13561 catgacccat ttcgcgccag ttggtggcga tcatgttagt cgcgacgggc aagccggtcg
    13621 cccgtcgaaa ctccgccatc acttcacgtc cggagaagcc ctgttctgcg ccgcatggat
    13681 cttcggcata ggtaagaaca tcattcagcc ctttgcataa agaaatggct tcatcaagca
    13741 gccatgcacc gttgggatca acggtaatcc gcgcatccgg gaagcttttc ttcaatgcac
    13801 gaacagtgtc gatttcttgc tcgccaggta acaccccgcc cttaagtttg aaatctttaa
    13861 agccatagcg atcctgtgag gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt
    13921 tctgatggcg caactgatac cactcatggt tgcccggcgt attttccaga taaggaagat
    13981 cggttttggt ccgatcaccg atataaaaca gataaccgag gacggtaata gtctcgcgtt
    14041 gcttgcctgg ccccaacagt tcgcagaccg gaacattcag cgccttacct agcaggtcaa
    14101 gcaaggcggc ttccagcgcc gccacggcat taacgcgcaa ttcaaaagtc caggcacctt
    14161 tgccgaaggt atcaaaatcg gctgcctgat tacctttatg cacctgctga accactttat
    14221 tcaggcgcgc aacttcctgg cccagaacca tcggaatagc atcgaccagt gtttgataaa
    14281 tcacctctcc gcccggcgct tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca
    14341 cgatattgcg ggtgaagtat gcgttatgcg caccaccaat attgagcaac atgctgtcat
    14401 gtccggcgac cggaatgact ttcatatcag taataacagg actggattgt gtcgccatct
    14461 tattgccccg cgacagattt cagctcgata cgcttgatat cgcccaccag caccaggtag
    14521 ctcagtaccg cgattagggc atgaacacca acataaatca gcgccccatt aaacgagcca
    14581 gtcgtgccaa cgatataacc aattgcgatt ggcgtgacga tgccagaaat gttaccgaac
    14641 atgttgaaca ggccaccgga aagaccgctg atctcttttg gcgcggtatc tgccattact
    14701 gcccaaccca gcgccccgat gcccttaccg aagaaggcca gcgccataaa gccgatgatc
    14761 atccactcaa cgttgacgta gttgcagaac accatcacca tcgacagcaa catgcccatt
    14821 acgatcggtg ttttacgcgc aatgttcagc gatcccgtgc ggcgcatcag ccagtcggaa
    14881 ataattccac ccagcacacc gccgataaaa ccgcaaaccg ccggaacgga agccacaaag
    14941 cccgctttca gaatcgacat cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg
    15001 aaaaagtaag tcagggcgtt gatacagtac tgaccgatat aaacgccgat catcatccgt
    15061 gacccgagca gctgtttgat ctgcccccac ttcacgctga acggcacttt aactttggtg
    15121 ttttgctgat ccatattgat cagcgcgcca cccgcggcga tatactccag ctctttctga
    15181 tttacccccg gatgttgatt tggctcgtgg atgactttca accagataaa gctgatgaca
    15241 atgcccagac cgcccataaa gaagaagacg tgtgaccagc ccacttcatg cgtcagccag
    15301 cccataatcg gcgcgaagat caccgttgcg aagtattgag cggagttaaa aatcgacacc
    15361 gccgttcccc tttcctgcgc cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa
    15421 gatggcgctt cagcaagccc gaccaggaag cgcagagtaa acagggcaac gataatgccg
    15481 aatccactaa agatatcgac gaagccttgc agcaaggtaa acatcgacca gataaagatc
    15541 gaccagaagt agacgcgttt tgaaccaaaa cgatccagca accaaccacc agggatctgc
    15601 ccgataacat aagcccatga gaaggcagag aacacatagc ccattcccac gggatcaagg
    15661 ccgatatctt tggccatttc cgaaccggca atagaaagcg tagctcggtc gccgtagttg
    15721 aaggatgtga cgataaacaa catcaccact atccagtaac gagcattcgt gcgtttttcc
    15781 acactgctcg cagcctgact taaagaactc attgttgcac tcctgaaaat tcgcgttagc
    15841 cacgctcact ctggactgcg acatcgccag gaaatcagag gtgacgtagg gtgttttttg
    15901 ccgtttttat aggtcgttcg ccgaatacgg cgcgtgttta tatctggcaa tagcagtata
    15961 aaaagcgcgc catagcggct caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc
    16021 cgattttggc attagcccta gacggtggaa tccacttcac ggaaatgaaa acaagaacaa
    16081 gaaaggaagg gtaaaaacga agaaataaag aagagtatga aatggatcgc ttgactctag
    16141 gcaaacgcca gtaaaaatcc gcgctatgaa gcagttttta ctggcatttg cctgaaaaga
    16201 ttcgattcag caccgctaaa acgacattta ccgctcgctg acatatcagg acaacagcgt
    16261 gccccactgt tcgacccacg gatttgattc cgtttccggt tccgggttct cactggcgtc
    16321 aatcaacagc atttcgccaa cccgctgagc gctctgttcc tgcaacaagg catcgaactg
    16381 tttgccgcca ttgcaaaaat ttacataact gctgtcgccg agcgcaatca cgccataacg
    16441 cagattcggc tggaagccca gactatcttt gattccctga aagagcggca caatgctatc
    16501 aggaaggtcg ccctgcccgg tcgtggacgt aaccaccaga acatacttat cctgataggg
    16561 cagccagtcg cttaattcag gatcttcaaa taccgttgct ttgtggccct gtgcggtcag
    16621 aattgcttct gcttcttccg ccactaacag cgaatttccg tacatggtgc cgacaaaaat
    16681 accaatttcc gccataccgt tttctccctg gattaggaac ttatctctcc atcctgaccc
    16741 gatggcgcac tgaactcaac cctttcattt tcaggaagca gaccgcgcca gccaaattgt
    16801 gataacgcct gcatccaggt gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta
    16861 aaaggatgcg tcaatgacaa ctgactggca tgtagcatta accgctggag gccaaaatgc
    16921 tcagcaccgc tgcgattctg gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga
    16981 cgcaaatggg caagatgtcg gcgaagctga tgtttgcgtc cggttttcgg ctccagttcc
    17041 accaggccgt agcgcgtggt cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc
    17101 agaccgcgat aatgcgtcac tgctggctgc gggcctttat cttcgcgggc aaatttatca
    17161 gcgattttgt ccagttcttc caccagtgga taatccaaca ccgcttcttc catcaaccag
    17221 ccgcgcacaa tcgcatggta acgtttctgg atttggtgct gttcaaactg ttgtgccagc
    17281 agccgtccgg cctcgctgga taatcccatc aacaacacac cagaagtggg tcggtccaga
    17341 cgatgagcag taaaaacatg ctgtcctatc tggtcacgca cggtttgcat gaccactact
    17401 ttctcgtcgc gatccagcca gctacggtga accagccagc cggagggttt atttaccgca
    17461 accagccatt catcctgata gagtatttcc agcattagct cgcatcatcc gcaaaaagag
    17521 catccagttt ttccaactca gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg
    17581 ccatttcata atagggtgca acagcaaaag cgcccggtaa cggctggttg ttatttaaca
    17641 aatcgtgcat tcgcgggatc agcccccact gcaaccactc cagtggttcc atggtgtcca
    17701 taaagaacgg ttgggtacta ttaaattgat gcggctgggg ttcatcgttt cgccagtgct
    17761 gatgttcacg cagtaacgct tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg
    17821 tcataaagca acctcaataa gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga
    17881 gcactgtatt cacagtgctc ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc
    17941 ctgctcatcc ttgacaactt ttcctctggc cttgcggcca atcgttcatc ctgaactatt
    18001 gcttcctgct cacaccaccc cgatgtgata cttcatcctg aagtgtccct ggccttcctg
    18061 acccaccgaa tcatcctgac cggttctcat tctccatcct ggaggtgtcc tttaacgcgt
    18121 cctgcgtcat cctcttcgct tcatccagaa gcctttccct gaaacaccat cctggtgtgt
    18181 cctgcagaag tgtcatcatc ctgatgttca cttcgttgtc tgactccctg tcgacgaaga
    18241 tagaatcgtc tttttcgcga agtcttacaa ggcgcttaca gacaatgcct taaggaaaat
    18301 ttcatatgaa agttaagaat taacaatcaa cattttgatt ttaaatgaaa ataacgattc
    18361 ttatcttttt aagtattcgc ttatttcctg gcgatctcct acagagtctg taagagatct
    18421 ctcacaacca gatggggtat ttggattaca gaagaggctt aagttgatta aggaattccg
    18481 caagatttga ggctaaatgc gtccgtttac gtgttcccag cgtctctttg cacacttcac
    18541 cggagagatt acacaccgag atgacctcta actcttcttc cagcgtcgcg ataaatagcg
    18601 tgggcggcag ttttagcctt ttctgagtca ccagatgacc aatcagattt tcctgcaccc
    18661 gacggaagtc gtcttcactc caggtttgca gcaatgtcag tttgatatcg ccaaactgcg
    18721 cgtgcatatc cccggcaaac tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa
    18781 tatcaaaagc gcgttcaacc gcgtttacat tttgttcccc ggtaaacggc tggggttgcc
    18841 agtatacggc atcttcggta gttgaaataa tgcacggtga aggaacaccg tacagttcct
    18901 cacttaacgg ccagctttta tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat
    18961 ctttcagggc ttgtgcggtc aaatcgtcca ctaatttctc tcttcactta aaccagatac
    19021 acttgtcgtt tagtttatct ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc
    19081 aggcacttgc gggcctgact cttggaaaat caaccgatta ccgggatacc tatgacgcca
    19141 gcctactgca aggcgttcca cgcagcctga atcgcgaccc gctgggtctg aaagcggata
    19201 acctgccttt tcacggtacg gatatctgga cgctgtatga actttcctgg ctgaatgcga
    19261 aaggtttgcc gcaggtcgct gtcggtcatg ttgaacttga ttacaccagc gtaaatctga
    19321 ttgagtcgaa gagttttaag ctctatctca acagttttaa ccagacgcgt tttaataact
    19381 gggatgaggt gcgccagacg ctggagcgcg acttaagcac ttgcgctcag ggtaagatta
    19441 gcgtggcgtt atatcgtctt gatgaactgg aaggccagcc gataggtcat ttcaatggca
    19501 cttgcattga tgaccaggat atcactatcg ataactatga attcactact gactatctgg
    19561 agaatgccac cagcggtgaa aaagtagtgg aagagacgct cgtcagccac ctgctgaaat
    19621 caaactgcct gatcacccat caaccagatt ggggttcgat ccaaattcag tatcgtggac
    19681 gccaaattga cagagaaaaa ctgctgcgtt acctggtctc attccgtcat cacaacgagt
    19741 tccacgaaca gtgcgtggaa cgcatcttta atgacctgtt acgcttctgc cagccagaga
    19801 aattgagcgt ttacgcacgt tatacccgtc gtggcggtct ggacattaac ccgtggcgca
    19861 gtaatagcga ttttgtccca tcgaccacaa gactggttcg gcaataaatt ttttctcaat
    19921 tttgcgtgct ggattcacgc agaaggttgt gaaaggtcat caggcagggc tattgtaatc
    19981 aaagggaatg acgatattcg tcccataagg agtttttctt gattacacat attagcccgc
    20041 ttggctccat ggatatgttg tcgcagctgg aagtggatat gcttaaacgc accgccagca
    20101 gcgacctcta tcaactgttt cgcaactgtt cacttgccgt actgaactcc ggtagtttga
    20161 ccgataacag caaagaattg ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc
    20221 gtgaacgcgg cgtaaagctg gaactgatta atcccccgga agaggctttt gtcgatgggc
    20281 gaattattcg cgctttgcag gccaacttgt tcgcggttct gcgagacatt ctcttcgttt
    20341 acgggcaaat ccataatacc gttcgttttc ccaacctgaa tctcgacaac tccgtccaca
    20401 tcactaacct ggtcttttcc atcttgcgta acgctcgcgc gctgcatgtg ggtgaagcgc
    20461 caaatatggt ggtctgctgg ggcggtcact caattaacga aaacgagtat ttgtatgccc
    20521 gtcgcgtcgg gaaccagctg ggcctgcgtg agctgaatat ctgcaccggc tgtggtccag
    20581 gagcgatgga agcgccgatg aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag
    20641 acagtcgttt tattggtatg acagagccgt cgattatcgc cgctgaaccg cctaacccgc
    20701 tggtcaacga attgatcatc atgccggata tcgaaaaacg tctggaagcg tttgtccgta
    20761 tcgctcacgg catcattatc ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt
    20821 tgctgggaat tttaatgaac ccggccaaca aagatcaggt tttaccattg atcctcaccg
    20881 gcccgaaaga gagcgccgac tacttccgcg tactggacga gtttgtcgta catacgctgg
    20941 gcgaaaacgc gcgccgccat taccgcatca tcattgatga cgccgctgaa gtcgctcgtc
    21001 agatgaaaaa atcgatgccg ctggtgaaag aaaatcgccg tgatacaggc gatgcctaca
    21061 gctttaactg gtcaatgcgc attgcgccag atttgcaaat gccgtttgag ccgtctcacg
    21121 agaatatggc taatctgaag ctttacccgg atcaacctgt tgaagtgctg gctgccgacc
    21181 tgcgccgtgc gttctccggt attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca
    21241 ttgaagagtt tggtccttac aaaatcaacg gcgataaaga gattatgcgt cgtatggacg
    21301 acctgctaca gggttttgtt gcccagcatc gtatgaagtt gccaggctca gcctacatcc
    21361 cttgctacga aatctgcacg taatctcccc tcccc
//
LOCUS       DADLSA010000057        21264 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.057, whole genome shotgun sequence.
ACCESSION   DADLSA010000057 DADLSA010000000
VERSION     DADLSA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.057"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            14..922
                     /locus_tag="JN691_002159"
     CDS             14..922
                     /locus_tag="JN691_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243605.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1161..1220
                     /locus_tag="JN691_002160"
     CDS             1161..1220
                     /locus_tag="JN691_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7243606.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1537..1596
                     /gene="ibsE"
                     /locus_tag="JN691_002161"
     CDS             1537..1596
                     /gene="ibsE"
                     /locus_tag="JN691_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HAY7243607.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1716..3149)
                     /gene="hldE"
                     /locus_tag="JN691_002162"
     CDS             complement(1716..3149)
                     /gene="hldE"
                     /locus_tag="JN691_002162"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAY7243608.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3197..6037)
                     /gene="glnE"
                     /locus_tag="JN691_002163"
     CDS             complement(3197..6037)
                     /gene="glnE"
                     /locus_tag="JN691_002163"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAY7243609.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDVAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SRLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVSARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6060..7361)
                     /locus_tag="JN691_002164"
     CDS             complement(6060..7361)
                     /locus_tag="JN691_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAY7243610.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVMAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7603..8223
                     /locus_tag="JN691_002165"
     CDS             7603..8223
                     /locus_tag="JN691_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAY7243611.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8287..9525
                     /gene="cca"
                     /locus_tag="JN691_002166"
     CDS             8287..9525
                     /gene="cca"
                     /locus_tag="JN691_002166"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAY7243612.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9688..10509)
                     /gene="bacA"
                     /locus_tag="JN691_002167"
     CDS             complement(9688..10509)
                     /gene="bacA"
                     /locus_tag="JN691_002167"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY7243613.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10600..10968)
                     /gene="folB"
                     /locus_tag="JN691_002168"
     CDS             complement(10600..10968)
                     /gene="folB"
                     /locus_tag="JN691_002168"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAY7243614.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11073..11690
                     /gene="plsY"
                     /locus_tag="JN691_002169"
     CDS             11073..11690
                     /gene="plsY"
                     /locus_tag="JN691_002169"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAY7243615.1"
                     /translation="MSAIALGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11703..12635)
                     /gene="ttdR"
                     /locus_tag="JN691_002170"
                     /gene_synonym="dan"
     CDS             complement(11703..12635)
                     /gene="ttdR"
                     /locus_tag="JN691_002170"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HAY7243616.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            12842..13685
                     /gene="ttdA"
                     /locus_tag="JN691_002171"
                     /pseudo
     CDS             12842..13685
                     /gene="ttdA"
                     /locus_tag="JN691_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13682..14287
                     /gene="ttdB"
                     /locus_tag="JN691_002172"
     CDS             13682..14287
                     /gene="ttdB"
                     /locus_tag="JN691_002172"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAY7243617.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14335..15798
                     /locus_tag="JN691_002173"
     CDS             14335..15798
                     /locus_tag="JN691_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAY7243618.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLLIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(15841..16854)
                     /gene="tsaD"
                     /locus_tag="JN691_002174"
     CDS             complement(15841..16854)
                     /gene="tsaD"
                     /locus_tag="JN691_002174"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAY7243619.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17092..17307
                     /gene="rpsU"
                     /locus_tag="JN691_002175"
     CDS             17092..17307
                     /gene="rpsU"
                     /locus_tag="JN691_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAY7243620.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17418..19163
                     /gene="dnaG"
                     /locus_tag="JN691_002176"
     CDS             17418..19163
                     /gene="dnaG"
                     /locus_tag="JN691_002176"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY7243621.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19358..21199
                     /gene="rpoD"
                     /locus_tag="JN691_002177"
     CDS             19358..21199
                     /gene="rpoD"
                     /locus_tag="JN691_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAY7243622.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5118 a         5881 c         5465 g         4800 t
ORIGIN      
        1 acaagaagcg tttatgacgc ttgagcagga gcagcaggtt aaaacccgta ctgccgaaca
       61 gaatgcacgt attgcggctt ttgaagctga acgtcgtcgt gaagcagagc agacgcgaat
      121 tctggctgaa cgacaaattc aggaaacgga gatcgagcgc gagcaggccg tccgctcaag
      181 aaaggttgag gctgaacgtg aagttcgtat taaagagatc gaacagcagc aggtcaccga
      241 aatcgccaac cagacgaaat cgatcgctat tgccgccaaa tcggaacaac agtcccaggc
      301 agaagcgcgt gctaaccttg cactcgcaga agcagtaagt gcgcaacaaa acgtagaaac
      361 cactcgccag accgcagaag ccgatcgtgc taaacaagtt gccctaatcg ctgccgcgca
      421 ggatgcagaa accaaagcgg ttgaactgac cgtgcgggcg aaagcagaaa aagaagccgc
      481 agagatgcag gcggcggcta tcgttgagtt agccgaagct acacgtaaaa agggtctggc
      541 ggaagcagaa gcacaacgtg cgctgaacga tgctatcaac gtactttctg atgaacaaac
      601 cagccttaaa ttcaaactgg ccttgttgca ggcgctacct gcggtaatag aaaaatccgt
      661 tgagccgatg aaatctattg acggtatcaa gattattcag gtcgatggtc tgaatcgtgg
      721 cagcgctgcg ggtgatgcaa acacgggtaa tgtggggggc ggaaacctag cggaacaagc
      781 attatcagcc gctctctctt accgcacaca ggcaccgctg attgactcct tgctcaatga
      841 aattggcgtt tcaggcggtt cactggcggc attgacttca cccttaacct caacaactcc
      901 cgtcgccgaa aacgtagaat aaattttcag ctatgaacgt ccctagcaca aaaagccggg
      961 gattttttat atctgcgttc cgctaaaaac tgcaaatgat caggtcgttg caacgttttg
     1021 cgtgaccgct cggggaaggc aaaactgcct ctgggaaagc gttgcgcgag gtccggcgct
     1081 catcaacaat cggggagtca gcaaagggct gaaacgggaa agcccctccc gaggaagggg
     1141 ccttacagaa ggaaagggtt atgatgaagc tcgtcatcat actgattgtg ttgttactcg
     1201 taagtttcgc agcttattaa cagccaatca gaggggggag aaatcctccc tcacccttgt
     1261 tcctttactt taggttgaaa aaaaatcagc gtcaagaagg tttcattaac acatatccct
     1321 ggcccaaaaa gccggggatt ttttatatct gcgttccgct aaaaggtgca aatgctcagg
     1381 acgttgcagc gttttgcgtg accgctcggg gaaggcaaaa ctgcttcagg gaaagcgttg
     1441 cgcggggtcc ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa cgggaaagcc
     1501 cctcccgagg aaggggcctt atagaaggaa agggttatga tgaagttcgt catcatactg
     1561 gttgtgttgt tactgttaag tttcccgact tactaacaac tgatcagagg ggggagaaat
     1621 cctcccttac ccttgttcct ttactttagg ttgaaaaaac aacagcgtca ataggcctgc
     1681 ctgctacgaa gcgagatctg tgaaccgctt tccggttagc cttttttatc ctgttggatc
     1741 ttcttgatga tgttagtggt cgagcaaccg tcttcgaagt tgagcaccag cacttcgccg
     1801 ccgttggccc agacttcttt acttccggcg atctcttctg gtttatagtc gccccccttc
     1861 accagtaaat ctggcaggat cccggcgatc aagcgctgcg gcgtgtcttc ttcaaacgac
     1921 accacccagt cgaccgcttc cagtgcgccc agcacaatca tacgctgttc gagcgggtta
     1981 acggggcggg aatccccttt cagccgtttg gtggaggcat cgctgttcac agcgacaatc
     2041 aagcggtcac ccagtttgcg ggcatttgcc agataggaga cgtgtccggc gtgcaggatg
     2101 tcaaagacgc cattggtcat caccactttt tcaccgcgtt tacgcgctgc cgctacggcc
     2161 agcttcagtt cctcttcggt catcacgcca aagccggtat ctgcgcgccc acgcactgca
     2221 ttttccagct cgatcggcga aaccgtggag gttcccagtt tgccgaccac cacgccagcc
     2281 gccgcattgg caaagaagca ggcttcttcc agcgaattac ccgctgccag cgttgccgcc
     2341 aggacgccaa tcaccgtgtc gcctgcaccg gtaacgtcat acacttcctg cgcctgggtt
     2401 ggcatatgca gcggcgcttt acccggttgc agcagcgaca taccctgttc ggaacgggtc
     2461 actaacagag ccgagagttc gtaatcggca atcagtttca tgccgcgctc aacaatctct
     2521 tcttcggtct tacatttacc gacaacagct tcaaattccg agagattcgg tgtcagcagc
     2581 gtagcgccgc ggtagcgctc aaaatcggta ccttttggat caatcagcac cggaacgccc
     2641 gctttacgcg ccagttggat catctgctgt acgcttgcca gcgcaccttt ggcgtagtca
     2701 gaaagcacca gcgcgccaat cgaactcagc gcctgattaa tccgctcgtg cagcggctgc
     2761 ggatcaacac cttcgaaacc ttcttcaaaa tccagacgga tcagctgttg gttgcgggaa
     2821 agtacccgta atttggtgat ggtcggatgc gtcggtacag aaacgaagtc gcatttgacg
     2881 ttgacgtcgg ccagagattt actcagcgcg cgcgctgcat cgtcaatgcc cgtcaacccg
     2941 accaggcgtg cattagcacc gagagaagcg atattcatcg ccacgttagc cgcgccgccc
     3001 ggacgttctt cgatggtatt cactttaacc acgggcaccg gcgcttccgg cgagatacga
     3061 ctggtggggc cgtaccagta acgatccagc atcacatcac caaccaccat cactcctgca
     3121 cgttcaaact ctggcagcgt tactttcatt cctgtctcct gagagattca aaatttgcgc
     3181 gcgataatac catacttcat tcttccacca gccacttctg ccagcttgcc cgcaccagat
     3241 cacgctctgc ggtgaagcaa tcctccgaca catggcccgg caattcctgt aatgccagat
     3301 gatgaagttc atcgcgcagc gtcgtgtaag cacgggtcag cgccatcgct tcctgctctt
     3361 ccataatgtc gttttgcgcc agcagctcca gaatacgcac gttgtctgac cagcgcgtta
     3421 acttcggttt ttcatgagcg tagcgcaaca ccagatattg ggtaataaat tcgatatcgg
     3481 taattccgcc ttcatcggct ttgatatcaa agcgattacg atgtttattg ccgagatgag
     3541 cgcgcatttt ctcgcgcatt tcccgcactt ccgtttgcag agttttacct tctcgcggca
     3601 gtgtcataat ctcacgacgc accgcgtcaa agtgcgcagt gagctgcgga tcgccgtaca
     3661 ctacacgcgc actcaccagc gcctgatgtt cccacgtcca ggcctcgttt ttctgataat
     3721 cggcaaatgc ttctgcggat gtcaccagca ttcccgccgc cccggacgga cgcagtcgag
     3781 catccacttc atacaaaatg ccggaagagg tacgcgtact gaacagatgc ataatgcgtt
     3841 gcgccagacg cagataaaac tgccgcccgt cgatttcccg ctcgccgtca gtcatcgcat
     3901 ccattgggca atcatggagg aagataaggt caagatcgga actgtagcct aactcccagc
     3961 cgcccagctt gccgtagccg accaccgcaa aaccgcgccc ttcacgttcg ttgaggtgat
     4021 ttggcttacc gtagcgggcc accatttgac cccacgcctg ctgaacgacg gcatctatca
     4081 tagcttctgc cagccaggtt aagtgatcgc tcactttcat taccggtagc gtaccggcga
     4141 tatccgctgc cgcaatgcgt aacagctgcg cctgtttgaa ctgacgcagc gcctcaagct
     4201 gttgttcttc gtcatcttcc ggcacgcgca gcaaatactg gcgcagttca tcacggtagg
     4261 catcggtcgc cgtcggctgg taaagggtat ttggatcgag caattcatcc agcaataatg
     4321 gataacgcgc tagccggctg gcaatcatcg gcgatgcggc acacagagaa atcaaatgtt
     4381 taagcgccgc agggaattca ctgagcaatt ctaaataggt tgtgcgggta acaatcccca
     4441 ccagcaaggc ggtaatgcgc gacagcgtga cagcaacgtc ttcacgcgca cagacatcac
     4501 ttagcagatg tggcatcaga tggtcgagca cctgacgtcc tcgcggcccg atagtacgct
     4561 tatccagctc tttgcggaaa tcagcaatca gcgttaggac ctgtttgcga tcatcctcgc
     4621 taagatgcgc cagcactggc gtagtgtcat cttcctgcaa cgcatcctgc cacagctcac
     4681 gccactgttc cgacagcgac tcttcctgag tttcactttc gtcgtcgcca atcaattcat
     4741 taaacacccg gcgcacattg gtcatatgtg cggtcagcgc cccggtcagt tgcggccagt
     4801 cagcaaagtc catcgcccac gccagccgcg cacggttaag ctcatcagaa ggaagcgttt
     4861 gggtttgttc gtcgttaatg ctttgcagca ggttttccag acgccgcagg aacagatatg
     4921 ccactcgcaa ttgttcagca tcgttttcag aaagcagatg cagcgcggca atggcgctga
     4981 gcgttggcag taaagagcgc gactgcagcg acggttcgcg cccaccgcga atgagctgga
     5041 acacctgaac gataaattca atttcgcgaa tgccgcctgc gccgagtttg atattgtcgg
     5101 tcaaaccacg tcgacgcact tcacgggcaa tcatcccttt catgttgcgc agcgactgaa
     5161 tcacgctgaa atcgatgtaa cgacggaaaa caaacgggcg cagcatcgca cgcaactcgt
     5221 tagcatagac gccttcgcta tcgcccataa tccgcgcttt gaccatcgcg taacgctccc
     5281 agtcgcgtcc ctgctcctgg taataatcct ccaacgcggc aaagctcagc accagcgggc
     5341 cactttcgcc aaacggacgc agtcgcatat ccacgcgata gacgaagcca tcctgcgttg
     5401 gttgatccag cactttaatc agccgctgcc ccatgcgggt aaaaaactgc gcgttatcca
     5461 gttcccggcg tccgccctgc gtacaaccat gttccggcca ggcaaaaatc agatcgatat
     5521 cagaggagaa attcagttcc ccgccgccca gcttacccat gcctaagatc agcagcggtt
     5581 gcgcttcgcc ctgcgcattg cacggcgttc cccactcgcg gcagcaggcg tcatacagcc
     5641 agtcacgcgc cgcaacaatc agcgtctccg ccagataact gagctgctgc aatatgctct
     5701 cttcagtaac cagtgccagc gtttgcgccc atgcgatgcg caccataatg cgccgccgga
     5761 atagccgcag ctcgcgcatt aacccggctt cgtcgctcac attactgagc gcctcctgca
     5821 accacacgct gtaatgttgc cattcgtcag cctgtggcgg ttggctttcc agttccgtca
     5881 gccactctgg atgcgcaatc acgctgtcct gcacaaaatc actgaaagta agtactgact
     5941 tcgcctgtgc actaagtgat tcctcggcta aaagctctgg cagccgctca acaacggtct
     6001 gccagtactg ctgtaacggt gaagagagcg gcttcataag cgattttatc cttgcctgat
     6061 taacgttttc cgctgtgcaa ccagaacggt tcctgattgt ttgcctcatt acggaaatgt
     6121 tcaatttcaa tgcgttgccc ggtcgcaata gcgtgacgca gcccctgcca gttctccagc
     6181 caggcttgcg cgacgacagg atcatagtaa cccgccagca acagtattga gtcaatatca
     6241 cgcatcaggc gtggcaactg gtcacggtag cgatcgccta acggctggca gaaaacgctt
     6301 ttcagttcag cggcatggcg ggaaagatgg atatcggcaa agcgtttgaa ggagtcgcta
     6361 attttgccct gcgctttggc atctaaaaac ggctgccatg ctttgcttac cagccattcg
     6421 gtcaacgcca gcttcgccat tgccgtttcg gtagagtaga ccgccatcac ggcagaaacc
     6481 gcagaagcga tggtcgcttc gcattgagtc agcaaatcac gtaagtgagt gctcgcttta
     6541 cgcggcacaa tgccaccgaa cagcatcagt gtatgacgga ctaggctaat ggctgccagc
     6601 acctgctctt tcgccgcatc gttgccgcgt acccacagtt cttcatgata ctgccattgc
     6661 gctaacacca gctcgagcgc cgcttccagc ccctgttcca catcggcttt tgccgcaaca
     6721 tgcaaaatgg tggtcggttt gatttcacgc gccggattgc cctgcgccag atgatagcca
     6781 cgcgccgctt tgctcaggct gccctggcgt aatccggttt gcgataccag ttggttcgcc
     6841 agtttcagca ccgcgcgcgt gtcgccgcta agcagctcca gttccagctc gcagataggc
     6901 tcagcaaatt cacccgcttt cacttccccc tggtcgagag cgatttcaat tcggctgcca
     6961 tcgatctcta ctagccattt ttcgcgataa aaatcggtgc taaacagcgg ctgcacgcgg
     7021 gaagcgagat cggcaggcaa ttcgccgttc ggccagattt ccgtcggtaa ctgcgccagg
     7081 tcgagcgtcg gttcgctcaa cgccacgtta tattccgggc gctggtgtaa gccgcctgtc
     7141 actcttcctg caactttcat ggtcatctca tagcgaccgt tttcgccacg aatacgtaag
     7201 cccatatcgt gcctacgcag ccagttatcc ggcgtttcgt agtaaatatt cagcaactgc
     7261 acggggtcat ggtgctcgcc gcccagcgta ttgagatggt cacgcaacgc ctcaacggca
     7321 ctgtgattaa caataaactt taattcgatt tcctgagcca tggcctttac ttatcggtta
     7381 tgtcacataa gtgacgatga acggcgaact taatgcgatc ttttttgtca gtagatagta
     7441 ttttgcgcca aattgccatg caacgggcaa tttgacgggc gtaaaagttt gaagcagtgg
     7501 caagacctga cacagaggat tccgattgat gtcgaatgct ttgcgtagcg acactgatgc
     7561 cactactatc gttccacttt ccatgacaat aacgacagcc tgatgccaaa attacgcctg
     7621 atcggattaa ctttactcgc acttagcgcg actgccgtct cacacgctga agaaacgcgc
     7681 tatgtttctg acgaactgaa tacctgggtc cgtagcggtc cgggagatca ttatcgcctc
     7741 gtgggcacgg ttaacgccgg cgaggaagtg accttattac aaactgacgc caacaccaat
     7801 tatgcccagg tgaaagacag ctctggccgt accgcctgga tcccgttgaa acaacttagc
     7861 actgagccaa gcctgcgctc ccgtgtgcca gatctggaaa atcaggtcaa aaccctgacc
     7921 gataaactca ccaatatcga taacacctgg aatcagcgca ccgcagaaat gcagcaaaaa
     7981 gtggcgcaga gcgacagcgt gatcaacggg ttaaaagaag aaaatcagaa actgaaaaac
     8041 gagctgattg tcgcgcagaa aaaggtcgat gccgccagcg tacagctgga tgacaaacag
     8101 cgcaccatca tcatgcaatg gtttatgtat ggcggtggcg tgctggggct tggcttgctg
     8161 ctcggtctgg tactgccgca cctgatccca agccgcaaac gcaaagatcg ctggatgaac
     8221 taaatcgcct tcttcgttgc actgtcttat tattaggaat tgtgtatttg acgaagggat
     8281 gatggcgtga agatttatct ggtcggtggt gctgttcggg atgcattgtt agggctatcg
     8341 gtcaaagaca gagattgggt ggtggtcggc agtacgccac aggagatgct cgacgcgggc
     8401 taccagcagg taggccgcga ttttcctgtg tttctgcatc cgcaaacgca tgaagagtat
     8461 gcgctggcac gtaccgaacg gaaatccggt tccggttaca ccggttttac ttgctatgcc
     8521 gcaccggatg tcacgctgga agatgatctt aagcgtcgcg atctgaccat taatgcgctg
     8581 gcccaggacg ataacggcga gattatcgac ccgtacaacg gtctgggcga tctgcaaaat
     8641 cgtctgttgc gccatgtttc ccccgctttt ggcgaagatc cgttacgcgt actgcgcgtg
     8701 gcgcgttttg ctgcgcgtta tgcccacctc ggttttcgta ttgccgatga aactctgacg
     8761 ctgatgcgcg agatgaccca tgcgggtgaa ctggaacacc tgacgcctga acgggtatgg
     8821 aaagagacgg aaagcgccct caccacccgc aatccccagg tgttcttcca ggtactgcgc
     8881 gattgcggcg cacttcgcgt tttattcccg gaaattgacg cactgtttgg cgttccggca
     8941 cctgccaggt ggcatccgga aatcgacacg ggtattcata ccttaatgac gctctcaatg
     9001 gcggcgatgc tgagtccgca ggttgatgtc cgtttcgcaa ctttatgcca cgatctcggt
     9061 aaaggactga cgccgccaga actttggccg cgtcatcatg ggcatggccc ggcgggtgtt
     9121 aagttagtgg aacaactatg ccagcgtctg cgcgtgccaa atgaaattcg cgatttagcc
     9181 agactggtgg ctgagtttca cgatctcatc cacaccttcc caatgctgaa cccaaaaacc
     9241 atcgtcaaat tatttgattc catcgatgcc tggcgtaaac cgcagcgtgt cgagcaactg
     9301 gcgctgacca gcgaggctga cgtgcgcggc agaaccggtt ttgaatcagc ggactacccg
     9361 caaggccgtt ggttgcgcga agcctgggaa gtggcgcagt cagtgccgac aaaagcggtc
     9421 gttgaagcgg gatttaaagg cgtggagatt cgcgaggagc tgacccgacg gcggattgcg
     9481 gcggtagcca gctggaagga acaacgttgc ccaaagcctg actgagtata aaactaaacg
     9541 ttagcacgaa ccgtcccctc gccccttcgg ggagagggtt agggtgaggg gaacagacca
     9601 gcaccggtgc gaacaatcac ccccaccccg gccctctccc tacagagcga gggagaaaac
     9661 ccggcctgct cccgaacctt ttctcaatca aaagaacacg acatacaccg cagccgccac
     9721 aataaagcga taaatggcga acgggataaa cgaaatgcgc ttaatcaatt gcaggaaggt
     9781 tttaatcgct atcagcgcca ccacaaaagc ggtgataaac ccaacggcaa acatcgggat
     9841 atcgccggtt gtcaggaagc cccagctttt gtagagatcg agcgccgttg cgcccatcat
     9901 catcggtacc gccagcagga acgagaactc ggaagcagcg taacggctca cgcccatcag
     9961 catcccacct gaaatggtcg ccccggaacg ggagaaaccc ggccacagcg ccagacactg
    10021 gaaacagcca atcataaacg cctgacgata ggtcatatca tcaagacccg gcgcacgcgg
    10081 ctctttcggc ttcaggcatt cggcggcaat cagcaacaaa ccgccaacga ccagcgcata
    10141 catcacattt atcgggttaa acaatgactt aatcgtgtcg tggaataata gccccagtac
    10201 caccgccgga atcatcccca gcaaaatgtg gatcagcgtt aaacgacctt tgctttcacc
    10261 ttcgtgctgc aacgggcggc caaagtggat gccaatcagg ccaaacagac gccgccagaa
    10321 catcactact accgccagaa ttgatcctaa ctggatcaca acttcaaagg ttttcgccgt
    10381 gtcgccctca aaccccaaca agtgaccgac aataatcata tggcccgtgc tggataccgg
    10441 cagaaattct gtcaatcctt cgaccacacc caatattgcc gctatcagca gcgagtgcat
    10501 atcgctcatc aataaacccc taaattatta aaatgtaccg cttgtccgaa ctactgcagt
    10561 atgaccaggt tataaccgtt tggtttaaca gctgtaaaat taattatttt ctttcagatt
    10621 attgccacgc tcaatgatta cgccaacatt cgccgcccgc gccactgcgc ctggcttgct
    10681 gagtttgata cgcacccacg gcgagttgaa gcgtgctaac agcagctccg ccacctcttc
    10741 agccacgcgt tccaccagcg caaaacgcgc cccctcgacg tggctgacca ccgtttctgc
    10801 aatgtcagcg taactgaggc aatccgccac atcatcactt ttcgccgctt tacggttatc
    10861 ccatgccatt tcgatatcga acactaactt ctgttcgatg gtctgttccc agtcgtaaac
    10921 accaatagtg gtgattaccg aaagttgctc tataaataca atatccatca cgtcctgcct
    10981 gctttttggc taaccggata ccacttccgg cgaaatgtgc gtattatcca cagattcatc
    11041 gttgaacacg aattttcaaa acggaacagc ttatgagtgc aatcgcgctt ggaatgatcc
    11101 tcatcgcgta cctctgcggc tccatttcca gtgccattct ggtttgccgc ttgtgtgggc
    11161 tgcccgatcc gcgaaccagc ggctccggca atccaggcgc aaccaatgtg ttacgtatcg
    11221 gtggcaaggg agcagccgta gcagtactga ttttcgacgt tctgaaagga atgttgcctg
    11281 tctggggcgc gtatgaatta ggtgtcagcc ccttctggct aggcttaatt gccatcgccg
    11341 cctgtcttgg acacatctgg cccgttttct tcggttttaa aggaggaaaa ggcgttgcta
    11401 ccgcttttgg agcaatcgca ccaattggct gggatctcac cggagtaatg gcgggaacct
    11461 ggttactgac cgtgctattg agcggatact cgtcgctggg agcgattgtc agtgcactga
    11521 ttgctccgtt ttatgtctgg tggtttaagc cacaattcac cttcccggtt tcgatgctct
    11581 cttgcctgat cctgctgcgt catcatgaca acatccagcg tctgtggcgt cgtcaggaga
    11641 caaaaatctg gacgaaattc aaaagaaagc gcgaaaagga tcccgagtga tttctggtgg
    11701 atctacatga cctgatagcc ttcatcgggc ttgcccagcc gttgctggca ccatgccgcc
    11761 agaaattcca cgcagacacg taatttcatg ctgcgataga gcggctcccg gtaaacagcc
    11821 cagatattgg cgctttgtgc atactccggc aatacttgca ccagtttgcc actctccaga
    11881 aacggcaaca catcccactc ggaacgcagc ataatccctt tgccctccag cgcccattgc
    11941 aacactatct cgccgctatt ggaggaaaga tgcccgctaa ccttcaccga ttttttctcc
    12001 tgaccgttcc ccaactccca tattccgtgg gtcatatcgc gctctttggt caccagacag
    12061 tcatgacgac ttaattcctg taatgattgt ggctgcggat atttttgcag atactcaggt
    12121 gctgcgcata atattctttt atttttcgtt aacagatgcg caatataata atcaggaatt
    12181 tcgtcattaa tacgaatatc cagatcaata ttatcctgca ctaaatcaat ttgccgatcg
    12241 aacaattcaa aatgcacctg taactcagga taattgcgca taagttcggt aatggctggc
    12301 gcaatatggc tgcgcccaaa accaaaactg caaccaatac gaatcatccc ctccgggcgc
    12361 gttttgattt gcgtgacgtc atcgaccagt cgctgatatt gggtgaggat ttccagcgca
    12421 tgttcgtaac aacgttggcc actttcagtc agcgccacgc cgcgagcgga gcggttcagc
    12481 agcgttgttg ccagcgtatt ttcaaggatt tggatccgct tggtgacaaa tgcaggcgtt
    12541 tgcccaagcg tcgctgctgc agcactaaaa ctcccgctgt ggacgatttc taccagtacc
    12601 tgcaagtctt tggctaaagg ccagctattc agcatcgtta attatccgca gttgtgataa
    12661 gcgcagtgta tttcgcaaaa cattgccatt ccctgctctg gcgcaatatt cattcttatt
    12721 ttcgcgttga ttcacgaact gttaattaca tattcacggt ggcaaaaaat ataaaaccac
    12781 atttttagtg gtagtttgtg gcggtgaatt tttccagaca aatacaaaaa ctggagttgc
    12841 catgatgagc gaaagtaata agcaacaggc agtgaataag ttgacagaga ttgtcgctaa
    12901 ctttaccgcc atgatttcta cccgaatgcc tgatgacgtg gtggataaac taaaacagct
    12961 aaaggatgcc gaaacgtcgt cgatggggaa aattatctac catacgatgt tcgacaacat
    13021 gcaaaaagcg attgacctga atcgtcctgc ctgtcaggac accggggaga ttatgttctt
    13081 cgttaaagtc ggttcccgct tcccactgct tggcgagctg caaagcatac tcaaacaagc
    13141 cgtggaatag gcaaccgtca aagcgccact acgtcacaat gcggtagaaa tttttgacga
    13201 agtaaacacc ggcaaaaata ccggtagcgg cgtaccgtgg gtcacctggg acatcatccc
    13261 cgacaatgac gatgcggaaa tcgaagttta catggcaggc ggcggctgca cgctacctgg
    13321 ccgctcgaaa gtgttaatgc cgtcagaagg ctacgaaggc gtggtgaaat tcgtcttcga
    13381 aaatatctcc accctcgccg taaacgcctg tccaccggta ctggtgggcg tgggcatcgc
    13441 cacctcggtg gaaaccgccg ccgtactctc gcgtaaagcc attttgcgcc cgattggcag
    13501 ccgccatccc aatccaaaag cggcagaact ggagctacgc ctggaagaag gactcaaccg
    13561 tctggggatt ggtccacaag ggctgaccgg caacagttca gtgatgggcg catcgtcgcg
    13621 gcacgctgct ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt
    13681 tatgaaaaag atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcgg
    13741 cgatgtgatc tacctgaccg gtacgctggt gacctgccgc gacgtttgtc accgccgttt
    13801 gatcgaactg aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg
    13861 ccccatcgtg cgcaaaaacg gcgacaaatg ggagatggtc tccgtcggcc cgacaaccag
    13921 tatgcgtatg gaaagttttg aacgtgaatt tattgagcag accggcgtaa aactggtggt
    13981 tggcaaaggt ggtatggggc cgctgaccga agaaggctgc cagaaattca aggcgctaca
    14041 tgtgattttc ccggcaggct gcgcggtgct ggcggcaacc caggtagaag agattgaaga
    14101 agtgcactgg acagaactcg ggatgccgga gtcactgtgg gtctgccggg tcaaagagtt
    14161 cggcccgctg attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa
    14221 gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat
    14281 caaataaccc tcccggagag gctcacccct ctcctttttc gcaggcataa cacgatgaaa
    14341 ccttccactg aatggtggcg atatcttgcg ccgctggcgg tcatcgccat tattgctcta
    14401 cttccggttc ccgcagggct ggagaaccat acctggctct actttgccgt ttttactggc
    14461 gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg tggtggcgat ggtgggtatc
    14521 tccatcatcg ccatactctc tccctggctg ctgttcagcc ccgaacagct cgcccagcca
    14581 ggctttaagt tcaccgccaa atccctctcc tgggcagttt ctggtttttc taattcggtt
    14641 atctggctca ttttcgccgc ctttatgttt ggcacaggct atgaaaaaac cgggcttgga
    14701 cgccgcattg cgctgattct ggtgaaaaag atggggcatc gcacgctgtt tctcggctat
    14761 gcggtgatgt tctccgagct gatcctggca cctgtaacac cgtccaactc ggcgcgtggt
    14821 gcgggtatta tctatcccat catccgtaac ctgccaccgc tctatcaatc acaaccaaac
    14881 gacagcagtt cgcgcagcat tggctcgtac atcatgtgga tggggattgt tgccgactgc
    14941 gtgaccagcg ccattttcct gacggcgatg gcacctaact tgctgttaat tggactgatg
    15001 aaaagcgcat ctcacgccac gctgagttgg ggcgactggt tcctcgggat gttgccgctc
    15061 agcattttac tggttctgct ggttccctgg ctggcttacg tgctgtaccc gccggtactg
    15121 aagtctggtg atcaggtgcc gcgctgggca gagacggaac tgcaggcaat gggcccgctc
    15181 tgttcgcgtg aaaaacggat gctggggctg atggtaggcg cgctggtgct gtggattttc
    15241 ggcggtgatt atatcgatgc cgcgatggtc ggttacagcg tggtggcgct gatgctgctt
    15301 ctgctcatta tcagttggga cgacattgtc agtaataaag cggcatggaa cgttttcttc
    15361 tggctggcct cgcttatcac cctcgcgacc ggactcaaca ataccggttt tattagctgg
    15421 tttggcaaac tgttagcagg cagcttaagt ggttattcgc cgacgatggt gatggtgacg
    15481 ttgattgtgg tgttttatct actgcgctac tttttcgcca gcgccacggc gtatacctcc
    15541 gccctcgcac cgatgatgat cgccgccgcg ctggcgatgc cggaaatccc gctgccggtg
    15601 ttctgcctga tggttggcgc ggcaattggt ctggggagca ttcttacacc atacgccacc
    15661 ggacccagtc cgatttacta cggtagtggt tatctgccaa cggcggatta ctggcgactg
    15721 ggggcgattt ttgggctgat attcctcgtg ttgctggtga ttaccggctt actgtggatg
    15781 cccgtggtgt tgctttaagt gagggcggtg ccggaaattg ctctccggca cccaatagtt
    15841 ttacgcagcc ggtaactccg ccagcggcca gcgcggacgc acgctaacgc cgagatccgc
    15901 cgtcgcgcct gctttaaacc gcaccattcc ggcataggcg atcatcgcgc cgttatcagt
    15961 acaaaattcc ggacgcgcgt agaacacttc gccgcggcgt tttttcatca tttcagccag
    16021 cttcgcccgt aacgtgcggt tagcactcac gccgcccgcc atgaccagtc gcttaaagcc
    16081 cgtctgatcc aacgctcgct tacacttaat catcaacgta tcgaccaccg catcttcaaa
    16141 ggcgcgggcg atatcagcac gcgtctggtc gtcggtgccg ttgtcacgaa tcgtgttcgc
    16201 cgcaaaggtt ttcagaccag aaaagctgaa atccagcccc ggacggtcgg tcatcggacg
    16261 cgggaaaaca aagcgcccgg cagtaccctg agccgccatt ttcgacagta acggtccgcc
    16321 aggataatcc agccccagca gcttcgcggt tttatcaaac gcttcaccgg cggcatcatc
    16381 gatagactcg ccgagcagct cgtactgacc aatgccagtc acgctgatta actgcgtatg
    16441 accgccggac accagcagcg cgacaaacgg aaattccggc gggttatctt ccagcatcgg
    16501 cgctaacaga tgcccttcca tatggtgtac cgggattgcc ggaacgttcc aggcaaacgc
    16561 cagagaacgc cccacggttg cgccaaccag cagtgcgccg actaatccag ggcctgcggt
    16621 ataggccaca gcatcaatgt cttttgccgt taaaccagac tcctttagcg ccgcctggat
    16681 caacggtacg gttttacgca catgatcgcg ggaggccagt tcaggcacga cgccgccgta
    16741 gtcagcgtgc aatttcacct gactatacaa ttggttggct aacaaacctt tttcatcgtc
    16801 gtaaatggcg atgccggttt catcgcagga agtttcaata cccagtacac gcatgacttg
    16861 ttttacctcg ctttattacc gcgcagtgta ggaccaatgc gggttgatgt aaaactttgt
    16921 tcgcccctgg agaaagcctc gtgtatactc ctcaccctta taaaagtccc tttcaaaaaa
    16981 ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata
    17041 agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta
    17101 attaaagtac gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc
    17161 gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc
    17221 gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa
    17281 aacgcacgcc gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga
    17341 gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt
    17401 gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg
    17461 cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga
    17521 atttccacgc gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg
    17581 agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga
    17641 tgaactacga caagctcgag ttcgtcgaaa cggtcgaaga gctggcagca atgcacaatc
    17701 ttgaagtgcc atttgaagca ggcagcggcc ccagccagat cgagcgccat cagaggcaaa
    17761 cgctttatca gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg
    17821 ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgctc
    17881 gctttgcgat tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca
    17941 atccagaaaa tcgccagtca ttgattgatg cggggatgtt ggtcactaac gatcagggac
    18001 gcagttacga tcgtttccgc gaacgggtga tgttccccat tcgcgataaa cgcggtcggg
    18061 tgattggttt tggcgggcgc gtgctgggca acgatacccc caaatacctg aactcgccgg
    18121 aaacagacat tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata
    18181 acgctgaacc caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc
    18241 aatacggcat taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac
    18301 aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc
    18361 gcgatgccgc atggcgagcg ctggaaacgg cgctgcctta catgacagac ggccgtcagc
    18421 tacgctttat gtttttgccg gatggcgaag accctgacac gctggtacga aaagaaggta
    18481 aagaagcgtt tgaagcgcgg atggagcagg cgatgccact ctccgcattt ctgtttaaca
    18541 gtctgatgcc gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg
    18601 cactgccatt gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat
    18661 taggcaacaa attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg
    18721 cagagagcgg cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta
    18781 tagggttgct ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg
    18841 atgaaaataa gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc
    18901 agccaggtct gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca
    18961 cccttgaaaa actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct
    19021 tcaccgactc actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa
    19081 tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa
    19141 accaggagct ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc
    19201 cccgacagcc gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt
    19261 ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc
    19321 aacaccaacc tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca
    19381 gctgaaactt cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa
    19441 tgaccatctg ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat
    19501 caacgacatg ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc
    19561 tgaaaacacc gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga
    19621 atctgaaatc gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt
    19681 tgaactgttg acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa
    19741 ccaggttcaa tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta
    19801 cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc
    19861 gaacgcagaa gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga
    19921 agatctggac gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga
    19981 tgacaacagc atcgacccgg aactggctcg cgaaaaattt gcggaactac gcgctcagta
    20041 cgttgtaacg cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga
    20101 gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta
    20161 cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat
    20221 gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg
    20281 caacgaaacc agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga
    20341 aaaactgcac gatgtctctg aagaagtgca tcgcgccctg caaaaactgc agcagattga
    20401 agaagaaacc ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg
    20461 tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat
    20521 ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg
    20581 taacatcggc ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc
    20641 cacctacgca acctggtgga tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg
    20701 caccatccgt attccggtgc atatgattga gactatcaac aaactcaacc gtatttctcg
    20761 ccagatgctg caagagatgg gccgtgaacc gacgccggaa gaactggctg aacgtatgct
    20821 gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga
    20881 aacgccgatc ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct
    20941 cgagctgccg ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct
    21001 ggctggcctg accgcgcgtg aagcaaaagt tctgcgtatg cgtttcggta tcgatatgaa
    21061 caccgaccac acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg
    21121 tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgttgcg
    21181 tagcttcctg gacgattaat cggtaggccg gatcaggcgt tacgccgcac ccggcactag
    21241 tctctctgca caaacgccac cttt
//
LOCUS       DADLSA010000058        21038 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.058, whole genome shotgun sequence.
ACCESSION   DADLSA010000058 DADLSA010000000
VERSION     DADLSA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.058"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..584)
                     /locus_tag="JN691_002178"
     CDS             complement(<1..584)
                     /locus_tag="JN691_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7243623.1"
                     /translation="MNTTNGYNLALQRQLVDSKINDISDLKQKLEFSKIGSASDGMSV
                     TSTVEECEKHGKYTSYEKYLTISGKRITSSKSECPQCLEEKIRKKEIECEQAEQRARQ
                     SKIEYLLNSLNIPERFKNCTLQNYEPVNDDAKRVLKVCQAYASKWPERLQKGGGLVMC
                     GKPGTGKNHLALAIARHAIIEHQSSVIFTTALKIA"
     gene            complement(587..1453)
                     /locus_tag="JN691_002179"
     CDS             complement(587..1453)
                     /locus_tag="JN691_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243624.1"
                     /translation="MARSRNIKPGFFTNDELAECSPYARLLFAGLWTIADKEGRLDDR
                     PKKIKAMVLPFDDVDCNDLLQQLHSHKFINRYQVNGGSYIQINNWKKHQNPHCKEAAS
                     EIPEPVENNNSTMQVQCKDDKDEEKEDVESLQFIENNEAPEKHHASTVQEPVENSLNP
                     ADSLNLIPDSLSNTQAADATCEGGGADVHKISSRYAFEGNIVRLNHKDYEAWKRLYPN
                     IDLNYELERLDIEFSHEKPKNWFITASQKLSYQNKQALSRPVRKVANGLKAENFAAKD
                     YGQTEMPSWAQE"
     gene            complement(1465..2265)
                     /locus_tag="JN691_002180"
     CDS             complement(1465..2265)
                     /locus_tag="JN691_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7243625.1"
                     /translation="MAKKPTPDQIKKIRSGITKKIRFEVFKRDGFKCQYCGSSAPDVI
                     LHVDHINPVSKGGDNDMMNLITSCDSCNGGKSDKLLSDNSIMEKQRQQLQELNTKREQ
                     LEMMIKWRDGLKSLKDDVVDIVATKIDDCIAPFTVNDNGRKSIKRWLRIYKLEEILDA
                     IEIAADKKLTQEITHELTGEFFEYIPRIAATKRKPPKEQRILYIRGILKNRIYINQNH
                     VMSYLKAWLSYDLDLDELTEFAKTVPNWTTFKEWVSERIREAQKELPY"
     gene            complement(2287..2586)
                     /locus_tag="JN691_002181"
     CDS             complement(2287..2586)
                     /locus_tag="JN691_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243626.1"
                     /translation="MEQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGLTNKKRPVCKTERSEQIQMEF"
     gene            complement(2725..2949)
                     /locus_tag="JN691_002182"
     CDS             complement(2725..2949)
                     /locus_tag="JN691_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7243627.1"
                     /translation="MNLVIQRALKIVGSQKRLADKCGVTQPAVHKWLKGGQVSPEKVT
                     AIVNATGGQIKAYEIRPDLPHLFPKPNQAA"
     gene            3094..3768
                     /locus_tag="JN691_002183"
     CDS             3094..3768
                     /locus_tag="JN691_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7243628.1"
                     /translation="MRSAGVSQSQLADMVGISQPAIQKMSSGKTNGSRKMVELANALK
                     VRPEWLSSGIGEMRDGTHEEPSNIRESSLKAVVWEDIKKNDDEFVALPLLNVSLSAGS
                     GSCEIEESSEFSLVFRKYYLKKMGVTESSAKLVRVTGQSMEPTLHDGDVVGVNTQDTT
                     IRDGKTYAICQSDLLRVKTLIATPTSVIIRSINREEYPDEVMDRDEFHKTVRIIGRVF
                     WSSHSW"
     gene            3806..4147
                     /locus_tag="JN691_002184"
     CDS             3806..4147
                     /locus_tag="JN691_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HAY7243629.1"
                     /translation="MAFTDLEYQAVKKEVHQFIESIRPPEHIRNELDIVYSINDQTID
                     IGEQRPVWQGNPGETNILPSARIKYIRSLDRWKIYWMRKDMKWHQYSTELSLTDALEL
                     VRADPDCCFFG"
     gene            4679..4870
                     /locus_tag="JN691_002185"
     CDS             4679..4870
                     /locus_tag="JN691_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243630.1"
                     /translation="MAPKKKAVADVQVGVKTFELPIPLRGDLIVTIGNLPRDLTKDEA
                     KRISLIVESFAMIDGGTKE"
     gene            5675..6058
                     /locus_tag="JN691_002186"
     CDS             5675..6058
                     /locus_tag="JN691_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7243631.1"
                     /translation="MTRRTQFKGNSRSRRRERLKAKALANGVLTREEAISSEVLHRPT
                     LSRAQIQAKGTHETPERIEDAKPIKFMAQDVIWQQKEYRRNLERAAIVYANEFGHKQP
                     ETGVCLPNVALYAAGYRKSKQLTAR"
     gene            6120..6743
                     /locus_tag="JN691_002187"
     CDS             6120..6743
                     /locus_tag="JN691_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243632.1"
                     /translation="MSSIRLTTRIKEVIARNALIKSGVFTELEEVTKLKNQLALDARV
                     IAFGGKKKTEEVDQLSSRLMAISKELKKLGCSFYSCDVLSTSIYLAVSGRSVGWYSYG
                     KDSNGEDIWLPTPTRDKCMFDAGHEITKRFDEICALQQKLEARKKDIESNVWAALNSV
                     TTVKRLIEVWPESKELLPKEADKASTALPALRVEDLNKMIGLPSEAA"
     gene            6849..6989
                     /locus_tag="JN691_002188"
     CDS             6849..6989
                     /locus_tag="JN691_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HAY7243633.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDVLNQPGVPK
                     NG"
     gene            6982..7095
                     /locus_tag="JN691_002189"
     CDS             6982..7095
                     /locus_tag="JN691_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HAY7243634.1"
                     /translation="MDKTLMAIQTKFTIATFIGDEKMFREAVEAYKKWRSK"
     gene            7092..7280
                     /locus_tag="JN691_002190"
     CDS             7092..7280
                     /locus_tag="JN691_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243635.1"
                     /translation="MIPVELAKTPELSRLKREYHIAEARYWRKAGDKSKKQLCLWQAQ
                     RERMNEREFLSSPSELPF"
     gene            7289..7945
                     /locus_tag="JN691_002191"
     CDS             7289..7945
                     /locus_tag="JN691_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020753290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7243636.1"
                     /translation="MGTATLILGESGTGKSTSLRNLPPEKTLIIQCINKPLPFPSSDW
                     VKISKENNNGNIYRTDNTENIVRALINAPHEIVVIDDYQAVMVNELMGKCNERGYDKF
                     TSIGKNAWDVFRAAGEGADTRRVYILAHTQTDDFGYTRMKTVGKLVDSTLVPEGYFTI
                     VLRTHVQNGKYSFSTQTNGSDCCKSPVGMFDSQFIDNDLYEVDKKICSYYGFEKNETS
                     "
     gene            7932..8537
                     /locus_tag="JN691_002192"
     CDS             7932..8537
                     /locus_tag="JN691_002192"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243637.1"
                     /translation="MRRLEVSGERFGNLVVIGDAGMRGTQRLVNCVCDCGVVCVKQLG
                     NLRSGHTRSCGCSRSSTTKARKNKHGMYGTPTYKSWSSMLTRCFNERNSKYKHYGGRG
                     ITVCERWLDFKLFFADMGKRPDGTTLGRIDNNGNYEPSNCEWQKSIEQARNKSNTVLF
                     KFNGVLATISEHCEKTGLNPSTVRSRIYTYHWSVDKALSKG"
     gene            8542..9129
                     /locus_tag="JN691_002193"
     CDS             8542..9129
                     /locus_tag="JN691_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HAY7243638.1"
                     /translation="MSNVIFTYNEEAALTAGQGGFINETGAHIITITEAELKQSEKGA
                     KFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGANVVHAMMGCVGIKQLTQHMVSASK
                     FVAPEFHGKKIGLVLQKVLTTNKKTGADSYQMEIRIPFIAQTGQTLKEKSEGKQPETI
                     ANMVASLKDKDNRAKNVSQNHADDYGYSQNDYPPF"
     gene            9153..9449
                     /locus_tag="JN691_002194"
     CDS             9153..9449
                     /locus_tag="JN691_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HAY7243639.1"
                     /translation="MPAPLYGADDPRNCSGSSKAEVLENIKNNFDAFLALTPETKAER
                     MYRRDIQLALKQEKDRTNETAMRPLRKATIDKFPEYIDPRLRNYRSRYGAISND"
     gene            9460..9624
                     /locus_tag="JN691_002195"
     CDS             9460..9624
                     /locus_tag="JN691_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HAY7243640.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRNSFGSVNDNKY
                     LNAMWRSGKK"
     gene            9645..9974
                     /locus_tag="JN691_002196"
     CDS             9645..9974
                     /locus_tag="JN691_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001476141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eae protein"
                     /protein_id="HAY7243641.1"
                     /translation="MDGFLKGKCIPRDLKVNETNAEYLVRKFDEVRAEARNEGINYTA
                     SRLAAAFNHGFINKSLREVFDVTRMILSAKEELANEQHPIDGLSGGYAEKSLEEWAEQ
                     IRKGGNQ"
     gene            9971..10474
                     /locus_tag="JN691_002197"
     CDS             9971..10474
                     /locus_tag="JN691_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY7243642.1"
                     /translation="MSKIDYQALREAAEQATKGSYIVGHTSVNRHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAAFNPNVALALLDERERNLQYIKSRDQEN
                     EEIALTVGKLRVELEAAENNLIDSECHVAELEEALRDKQALLEASEKRIAELEAQTVT
                     VKEVGDA"
     gene            10547..11161
                     /locus_tag="JN691_002198"
                     /pseudo
     CDS             10547..11161
                     /locus_tag="JN691_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550159.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            11286..12068
                     /locus_tag="JN691_002199"
     CDS             11286..12068
                     /locus_tag="JN691_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000993708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY7243643.1"
                     /translation="MNTQISVETLSPITYKQTNVITSELLAQLYGTDVVRIRQNHNEN
                     KSRFVEGVHFYKVVGDELRALKHKVALNYFVKIAPNARHLILWTERGAARHAKMLETD
                     QAWDVFEKLEDGYFNQREKVANHPQPVPQPESSLDRARAMEIALNVADRLVNMFPNLS
                     SESKQAAVAGVFNPIVGRDVVPLPAISEHYYSATEVGQRYGISANKVGRIANTYMLKT
                     EKYGKWFIDKSAHNDKQVETFRYNEAGVHKIEELIEGGRKAA"
     gene            12394..13050
                     /locus_tag="JN691_002200"
     CDS             12394..13050
                     /locus_tag="JN691_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY7243644.1"
                     /translation="MATQISVESLSPITYNHIPVITTELLARLYGTETNNIKVNYTRN
                     AGRFVCGKHYFKLEGGELREFKNKVTHSNLVAPRAKHLILWTERGAARHAKMLETDQA
                     WEVFEKLEDCYFSPGKAEQHPQIQQQFTDEEIILLCYMQVQMENALDICKRLYPILKE
                     LNSSYTSKLYDIAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF
                     "
     gene            13135..13353
                     /locus_tag="JN691_002201"
     CDS             13135..13353
                     /locus_tag="JN691_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY7243645.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            13331..14401
                     /locus_tag="JN691_002202"
     CDS             13331..14401
                     /locus_tag="JN691_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7243646.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            14536..15819
                     /locus_tag="JN691_002203"
     CDS             14536..15819
                     /locus_tag="JN691_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAY7243647.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(15960..18140)
                     /locus_tag="JN691_002204"
     CDS             complement(15960..18140)
                     /locus_tag="JN691_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAY7243648.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(18323..19756)
                     /locus_tag="JN691_002205"
                     /pseudo
     CDS             complement(18323..19756)
                     /locus_tag="JN691_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
     gene            complement(19832..20884)
                     /locus_tag="JN691_002206"
     CDS             complement(19832..20884)
                     /locus_tag="JN691_002206"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HAY7243649.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
BASE COUNT         6031 a         4802 c         4930 g         5275 t
ORIGIN      
        1 gcaattttca acgcagtggt aaatatcaca gagctttgat gctcgataat ggcgtgccta
       61 gcgatagcca gtgcaagatg gttctttcca gttccaggct taccacacat gaccagtccg
      121 ccaccttttt gaaggcgttc aggccactta ctggcatatg cctggcatac cttaagaact
      181 cgctttgcat catcgttaac aggctcataa ttctgaagag tgcaattttt gaacctctct
      241 ggaatattga gagaattcaa caagtattca atttttgatt gtcttgctct ttgttctgcc
      301 tgctcacatt caatctcttt cttacgaatt ttctcctcaa ggcactgtgg gcactcagat
      361 ttacttgaag taattctttt tcctgagatg gtcagatatt tctcatagga agtatattta
      421 ccgtgtttct cgcactcttc cactgtgctg gtaactgaca ttccatctga tgctgatcca
      481 attttgctaa actcaagttt ctgtttcaga tcagaaatgt cattgatttt tgagtctaca
      541 agttgccttt gcagtgccag attgtaacca tttgtcgtat tcatattcac tcctgcgccc
      601 atgaaggcat ttcagtttgt ccgtaatctt tggcggcaaa gttttcagcc ttaagtccat
      661 tagcaacctt gcgtaccggt ctggataacg cctgcttgtt ctggtagctg agtttctggc
      721 tggcagtgat aaaccagttc tttggcttct catggctaaa ttcgatatcc agcctttcca
      781 gttcgtaatt caggtcaatg ttcggataca ggcgcttcca tgcttcgtag tccttgtggt
      841 ttaaccgaac gatatttcct tcgaaggcat agcgacttga aattttatgg acatcagccc
      901 cacccccttc acaagtcgcg tcagcggctt gggtgttaga aagggaatca ggaatcaggt
      961 taagggaatc agcaggattt aaactgttct ctactggttc ttgcactgta cttgcatggt
     1021 gcttttctgg tgcttcattg ttttcaatga attgcagtga ttctacatcc tccttctctt
     1081 catctttatc gtccttgcac tgtacttgca tggtgctgtt attgttctct actggttctg
     1141 gtatctcact ggcagcttct ttgcagtgag ggttctggtg ctttttccag ttattgattt
     1201 ggatatatga gccgccattt acctgatatc ggttgatgaa tttgtgactg tgaagttgct
     1261 gaagcagatc attgcagtcc acatcatcga aaggcagtac cattgcttta attttctttg
     1321 ggcgatcatc caaccgccct tctttgtcag caatggtcca taacccggca aaaaggagac
     1381 gggcatatgg agaacactca gctaattcat cattggtgaa aaagcctggt ttgatatttc
     1441 ttgaacgtgc cattttgtcc acctttagta cgggagttct ttctgagctt cacgaatgcg
     1501 ttcagatacc cactccttga acgttgtcca gttaggtact gttttcgcaa actcagtaag
     1561 ttcatcgaga tctaagtcgt atgaaagcca tgccttgaga taactcatta catggttttg
     1621 attgatatat atgcggtttt taaggattcc tctgatgtat agaatccttt gttcttttgg
     1681 cggttttctt tttgtcgctg ctatgcgagg gatgtactca aagaattcac cagtaagctc
     1741 atgggttatt tcttgggtga gttttttgtc tgcggcaatc tcaatcgcat cgaggatttc
     1801 ttcaagctta tatattcgaa gccatctctt gatagatttt cgcccattgt cattaacagt
     1861 aaatggcgca atgcagtcgt caattttggt agcgacaata tcaacaacat cgtctttcag
     1921 gctcttaaga ccatctcgcc acttgatcat catctccagt tgctctcgct tggtattaag
     1981 ttcctggagt tgctgccttt gcttttccat tattgagtta tcgctgagca gtttgtcgct
     2041 tttcccgcca ttacaactat cgcaagaagt aataaggttc atcatgtcgt tatcgccacc
     2101 cttgctaact gggttgatat ggtcgacatg aaggatgacg tctggcgcag aactcccaca
     2161 gtactggcat ttgaagccat cgcgcttgaa aacctcaaac cgtattttct tggtaatgcc
     2221 ggaacgaatc ttttttatct ggtctggtgt aggttttttt gccataatta actccgtaga
     2281 aagcagttaa aattccattt gtatttgttc agaacgctcg gtcttgcaca ccgggcgttt
     2341 tttattggtg agtccatcaa gcgcatactt aaaagccctg ctaatcggac tgatgtctga
     2401 tgccattccg aaagcacaca agaccgaagc aataaaccgc cagtcggtac gactgatctt
     2461 cgattcatga cagccaatca tctttgccag accgcgctgg gtaagcgttg acaggttgat
     2521 gagtaaatct gtttctgcac gatcaacgtc gcgctgtgat agtttgctgt aacttgtttg
     2581 ttccatttct taagatttcc aatagtgaat agttagttga aagctatgcg tggaaacgca
     2641 tatggcctta gttggtcaga tatcttgggg ctcgctttgt cagcgacgta ggacgaatgc
     2701 ccattgtgaa aatagcggtg ttacttatgc tgcctgattc ggttttggaa acaggtgtgg
     2761 taaatctggg cgaatttcgt aagccttgat ctgccctcca gtggcgttaa cgatggcggt
     2821 aactttctct ggagaaacct gcccgccttt cagccatttg tgtactgctg gctgcgttac
     2881 accacacttg tcggcaagcc gcttttgact accgacaatc ttcaaggctc gttgaattac
     2941 taaattcatg agcatacctc tttcagccat tacttataac caaggataac ccaagttata
     3001 aaagatagca ataaccttta ttattttact ttggataacc gtagttatag atttgtggat
     3061 atgaaaacat tcgcagaaag actaaatgca gccatgagat cggcaggggt gtcgcaatca
     3121 cagcttgcgg acatggttgg aatatctcag ccagccatac agaagatgtc atccggtaaa
     3181 acaaacggat ctcgcaagat ggttgagtta gctaatgctt taaaagtacg ccctgaatgg
     3241 cttagttctg gtattggtga aatgagggat ggtacacatg aagaaccatc caatatccgt
     3301 gagtcatctt taaaagctgt ggtatgggaa gacattaaaa aaaacgatga cgagtttgtc
     3361 gcattgcctc ttcttaacgt ttcgctttcg gctggaagcg gtagctgcga gatagaagaa
     3421 tcatcagagt tctctttggt tttcagaaag tactatctga aaaagatggg ggtaactgaa
     3481 agttctgcca agctggtaag ggtaacaggt caaagcatgg aaccaactct tcacgatggc
     3541 gatgttgttg gtgttaacac acaagatacc accatcagag atggtaaaac ctacgctatt
     3601 tgccagtctg atttgttgcg agtaaaaaca ttaatcgcca ctcctacatc ggtgataatc
     3661 agatcaataa atcgcgaaga gtacccggat gaagtaatgg atagagatga atttcataaa
     3721 accgtaagga ttattggcag agtattctgg tcatcgcata gctggtaacc agtggcctga
     3781 agaaacgttt gggtgaggag gatagatggc gttcactgac cttgaatatc aagcggtcaa
     3841 aaaagaagtt caccaattca ttgaaagcat aaggccgcct gaacatatcc gcaatgaact
     3901 ggatattgtt tatagcatca atgaccaaac gatagatatc ggcgaacagc gccccgtgtg
     3961 gcagggcaac ccaggtgaaa caaacatcct gccatcagca agaatcaagt acatacgttc
     4021 tctggataga tggaaaatct attggatgcg gaaggatatg aaatggcatc agtacagtac
     4081 tgaactttcg ctgactgatg cgcttgagct tgtgcgtgct gacccggatt gctgcttctt
     4141 cggatgagtg aagagacatt ttggtgatgg atagtcgcag aagtgcggcc tttaatgtaa
     4201 tcgaagaaaa gtaatccttg taaaacttga tttgactgta ttgtcacaaa gaaagaaatc
     4261 aacgatcgtt tcttgggtct tgatttgttt tgacgatcaa attagtattg cctcatcaac
     4321 tgtatgaggc ttttttatgc aaaccaaaag ctacaccatc aaagcatttc aggacttctt
     4381 acagatgctt cctgagcttg gtatcgtcaa tgatgctaca gcccgcaatc tccgtgactc
     4441 ttccctgcgc cttcttactg ttcttgggtc ggatttcacc gatgccgata tccgtgatta
     4501 tagcgttgcc aacttagcaa gttcgtacgc agactcagca gaaagcaaac cctctgaatc
     4561 atctttgcag gcatacaaaa gccggatgca aagtgctatt gataaattta tctcctacca
     4621 gaatggtgag attaatgtgt cagttggtga tgtaaataaa aaaaaggagc gtaaaaagat
     4681 ggcacctaag aagaaggcgg tagctgacgt acaggttggt gtaaaaacat ttgaactgcc
     4741 gattccattg cgcggagatc ttatcgtcac cattggcaac ctgcctcgcg acctgacaaa
     4801 agatgaagcc aagcgtatca gtctgattgt ggaatctttt gcgatgattg atggcggaac
     4861 aaaagaataa agccccgagg gaacggggct ttttgggtcg gaaatatatc cagtatttcc
     4921 gaagtgtatg gaggattacc taccgctaca cccttggcaa ccgcaagcca aggatagcgg
     4981 ttctcccaac aaactacaac ctgttcgggt aaacaggctg tatacaaaaa caggagaaaa
     5041 gctatggctc acgatgttaa tcgcgatgac ttcatctacg aacacgacaa gccggtttat
     5101 gtgcgcagtt actgccgcat acgtttcggt gagctcgaac atgttcgtca gcacttccgc
     5161 tcttatccga gctgctgaaa ataactaaat ctcaaacccg gcttccgcgc cgggttttct
     5221 ttgcctcgcg atcgtccccc atatttaaca gccgcaaatg cggtaaacca cgaaccaaca
     5281 cgccagcaat acagcgcccc ccttccaata ccgaccaatc cgcaacattt acaaaaataa
     5341 aataccttta ttatccatca cttataactt atttatcaga atccataaat taagttattg
     5401 accaccgcta taacctaggt tatctttaag ccatcagcag gacgcactac tcaccagggc
     5461 ggtgaatata caacgattcg aatatgaatc tacggcgctg acaaagcgca ataaccaaag
     5521 tgaactttgg ggtgtggtga agggttcatg gacgggaata tgtcgcacgt aaagcggcga
     5581 ggcctgcggg actattgccg aattgaagta ggccgaaaca ggtcaaaatg ggtctcccac
     5641 ctaccacacc accaaagttc atcaggaggt ctatatgaca cgcagaactc agttcaaagg
     5701 caattcacgt tctcgtcgtc gtgagcgttt aaaggcaaag gcattagcta acggcgtgct
     5761 gacccgcgaa gaagcaataa gttcagaagt attacaccgc cctactctaa gcagagcaca
     5821 gattcaggct aaaggtactc acgaaacgcc tgagcgcata gaagacgcta agccaattaa
     5881 gttcatggca caggacgtga tctggcaaca gaaagaatac agacgcaatc tggagcgagc
     5941 ggccattgtg tacgcgaatg agtttggaca taagcaacca gaaactggtg tatgtcttcc
     6001 aaacgtagct ctttacgcgg caggctacag gaaatccaaa cagctgacag caagatgact
     6061 tgtgttggtc gccagaaaat gaaatcaggc agcaaaccac ttatttgaga ggaattaata
     6121 tgtcatcaat ccgcttaaca acgagaataa aagaagtaat agcacgcaac gctttaatta
     6181 agtccggggt gttcactgaa cttgaagaag taacaaagct aaagaaccag cttgcacttg
     6241 atgccagagt tattgcgttt ggcgggaaga agaaaactga ggaagtggat cagttatcat
     6301 ccaggttaat ggctataagt aaagaactta aaaaactggg atgttcattt tactcatgcg
     6361 atgttctttc tacttcaatt tatctggccg tatctggcag aagtgttggc tggtattcat
     6421 acggaaaaga cagcaacggc gaagatatat ggctccctac tccgaccaga gataaatgca
     6481 tgtttgacgc agggcacgaa ataacaaaaa ggtttgatga aatctgcgca ttgcaacaaa
     6541 aacttgaagc caggaaaaag gatatcgaat caaatgtatg ggctgcttta aactcagtca
     6601 caacagttaa gcgacttatt gaagtttggc ctgaaagcaa agaattgcta ccaaaagaag
     6661 cagataaagc aagtacagca cttcctgctt tacgggtaga agatttgaat aagatgattg
     6721 gacttccttc cgaggccgca tagtcggcct ttatttttag cataaacaac agaataaaca
     6781 ctgcactgtg tattcattcc aaggagtgaa tacacggagc aatttaaagc gtaaataagg
     6841 agcacaccat gcaatatgcc attgcagggt ggcctgttgc tggctgccct tccgaatctt
     6901 tacttgaacg aatcacccgt aaattacgtg acggatggaa acgactcatc gacgtactta
     6961 atcagccagg agttcccaaa aatggataaa acacttatgg ctatccagac taaattcact
     7021 atcgccactt ttattggcga tgaaaagatg tttcgtgaag ccgtcgaagc ctataaaaaa
     7081 tggaggtcaa aatgattccg gtagagctgg cgaaaactcc agagttaagt cgattaaaaa
     7141 gagaatatca cattgctgag gctcgttact ggcgtaaagc gggagataaa tcaaagaaac
     7201 aactttgttt atggcaggca caaagagagc gcatgaatga gcgcgagttt ctttcctccc
     7261 catccgaatt accattctga ggcaaattat gggaactgcg acattaatac tcggtgaatc
     7321 tggtaccgga aaatcaacca gtttaagaaa cttaccacca gaaaaaacat taatcataca
     7381 gtgcataaac aaaccgctac cattcccatc aagtgattgg gttaaaataa gcaaggaaaa
     7441 caataacggg aacatataca gaacagataa tactgaaaat attgtaaggg cactaattaa
     7501 tgctccgcat gaaattgtag ttattgatga ttatcaggct gtgatggtta acgaactaat
     7561 gggtaaatgc aatgagagag gatatgacaa atttacctcg atagggaaaa acgcatggga
     7621 tgtgttcagg gctgctggtg aaggggcaga cactcggaga gtttatattc tggcacacac
     7681 gcaaactgat gattttggct atacacgaat gaaaaccgtc ggaaaactag tcgattcgac
     7741 acttgttcct gaaggttatt tcactatcgt gttaagaact cacgtacaga acggaaaata
     7801 ctcattttcc acccagacaa acgggagtga ttgttgtaaa agtccggttg ggatgtttga
     7861 tagtcagttt attgataatg atttgtacga agtagataaa aaaatatgct cttactatgg
     7921 gtttgaaaaa aatgagacgt cttgaggtat ccggcgagag atttggcaat ttagttgtta
     7981 ttggtgatgc aggaatgcgt ggtactcaaa ggttggtaaa ctgtgtgtgt gactgcggag
     8041 tagtttgtgt taaacagctt ggcaacctcc gttcagggca cacaagaagt tgtggttgct
     8101 cacgctcatc tacaacaaaa gcaagaaaaa acaaacatgg catgtatgga acgccaacat
     8161 acaagtcatg gtcttcaatg ttaacaagat gcttcaatga aagaaatagt aaatacaaac
     8221 actatggagg aagaggaata actgtttgtg aacggtggct ggactttaaa ttatttttcg
     8281 ctgatatggg aaaaagacca gacggaacta cattgggaag aattgataac aatggtaatt
     8341 acgaaccatc aaattgcgaa tggcagaaaa gtatcgagca agcaagaaat aagagtaata
     8401 ccgtattatt taaattcaat ggagtgctgg caacaatttc agagcactgc gaaaaaactg
     8461 gattaaaccc aagcacggta agaagcagga tttacacata tcattggtct gtagacaagg
     8521 ccttatcaaa aggataaaag catgagcaac gtgattttta cttataacga agaagcagca
     8581 ctgaccgcag ggcaaggtgg ttttattaac gaaactggcg ctcatatcat taccattact
     8641 gaagcagaac taaagcaatc agagaaagga gccaaattta ttgagttttc tggcgaatcc
     8701 gacgacggac gtaaaatcca atatcttagc gtttgtgttc agaaaaatga cggaacggaa
     8761 aacaagtttg gtgcaaacgt cgttcatgcc atgatggggt gtgttgggat taagcagtta
     8821 acgcaacata tggtttccgc cagtaaattt gttgctcctg aatttcatgg aaagaaaatc
     8881 gggttagtgc tccagaaagt attaaccaca aacaaaaaga ctggcgcaga cagctaccag
     8941 atggaaatac gcatcccgtt tattgcacaa acaggtcaaa cccttaaaga aaagtcggaa
     9001 ggcaagcaac cagaaactat cgccaacatg gttgccagcc tcaaagataa agacaatcgc
     9061 gctaaaaacg taagccagaa tcatgcagat gattatggtt acagccagaa cgattaccct
     9121 cctttctgat tactgaaaat aaggctccca ttatgccagc gcctctgtat ggtgcggatg
     9181 acccgcgcaa ctgctccggt agctccaagg cggaggtgct ggaaaatatc aaaaacaatt
     9241 tcgacgcgtt tcttgctctg acaccagaaa caaaagcaga acggatgtac cgacgcgata
     9301 tacaactcgc gctaaaacag gagaaggacc gaacaaacga aacagcaatg agaccgttgc
     9361 gaaaagcgac aatagacaaa ttccctgaat atatcgaccc gcgcctgcgt aattaccgct
     9421 cacgctatgg cgctatcagt aatgactgag gaatttacca tgagaggact tgcatacaat
     9481 cccggcattc ttccggcaga aatgattatt cgccaacgcg taaagccaat gccatcgaga
     9541 gaggaattgc ttaagcgaaa tagtttcggt tctgttaatg acaacaaata tctgaatgcg
     9601 atgtggcgga gtgggaagaa atgaaacaaa tgtcactaat tgagatggat ggatttctga
     9661 aaggtaaatg catcccacga gatttaaagg ttaacgaaac aaacgctgaa tatctggtgc
     9721 gtaaattcga tgaagtacgt gctgaggctc gcaacgaggg tattaactat accgcaagcc
     9781 gtcttgctgc tgcgttcaat cacggattta tcaataagtc tttgcgtgaa gttttcgacg
     9841 ttacacgcat gattttgtca gcgaaagaag agttggctaa tgaacagcat ccgattgatg
     9901 gcctgtctgg tggatatgcg gagaaatccc tagaagaatg ggcggaacag attcgcaaag
     9961 gaggcaacca gtgagcaaga ttgactatca ggcactgcgt gaggcggcag agcaggcaac
    10021 taaagggagc tacatcgtag ggcatacatc tgttaatcgg catggcaatt taacaggagt
    10081 ttttgtttgt caaaaatgga aaggagaacc cggtggtgtg attgcggaat gtcatgttaa
    10141 ctgcctggtt gaaacagatg ctcaggctta tgcaaacgct gaatttattg ctgcttttaa
    10201 tccaaatgtt gcgctggcac tactggatga acgggaaaga aacctgcaat acatcaaaag
    10261 ccgcgatcag gagaacgagg aaattgcgct aacggtaggg aagttgcgtg ttgagctgga
    10321 agcagcagag aacaacctta ttgatagtga atgccatgtt gctgaactgg aagaagcgct
    10381 acgcgataag caggcgttac ttgaagcctc agaaaagcgc atagcagaac tggaggcaca
    10441 gacagttacc gttaaagagg ttggagatgc ttgatagttt tgcgctcgtt gcactggttg
    10501 gcattgtagc gttgatttgg gtgacatccg ggaatcggag gcataagtga acgacaaaga
    10561 actgattaaa gaaatcaaag agcgtatcag cagtttggat gtgcgagaca atgttgaacg
    10621 ccgtgcttat gaaattgctc tggcctcgct gacagcagag ccgataggtg ctttccacat
    10681 tgcagaacag caggtcgatg gcacaagtga ttacatcaag gatggggatt ggcctattga
    10741 taatgggata attgaagtat acgccgcccc tccagcgcca atagtaccgg aagaaaaacc
    10801 aatgcctaat cctcttagca tgtacgcggt tgatgctgtt gccgctattg cagaggtgag
    10861 aggctggaac gcctgtcgtg ccgctatgct tcagagttaa gataattgca ttaaggatgc
    10921 ctggataagc tgtagtgagc gaatgccaga aatgggagag cgacaatgct atgtgttagc
    10981 agctgacttt aaaaacaact acccaccaaa catccccaac actcaggtcg gcgtatatgg
    11041 cgactggttt aatgatggca atcccacttg ggatgacggt gatggcgaag acctgtatct
    11101 caaagaggta acccactgga tgcagctacc agaaccgccg caggaggtta accgtggcta
    11161 acctgcaact tgccgtaaaa ggtgaataac aatcctcgca ttcgcggaga tttcttttat
    11221 ctgaactcgc tacggcgggt tcaccgtcaa gctttcttca atgcaaaagc caatgtgagg
    11281 acgctatgaa tactcaaatt tctgtcgaaa cactttcacc aatcacctac aaacaaacca
    11341 acgtcatcac cagcgaacta ttggcgcagt tgtacggaac ggatgttgtg cgcatccggc
    11401 aaaaccataa tgaaaacaag tcgcgctttg ttgagggggt acacttctac aaggtcgttg
    11461 gtgacgagct gcgcgcgcta aaacacaaag tagctttaaa ctactttgtg aaaattgccc
    11521 caaatgcacg ccacctcatc ctctggacag agcgcggagc cgcccgtcac gcgaagatgc
    11581 tcgaaacgga tcaggcatgg gacgtgttcg aaaaactgga ggacggctac ttcaatcagc
    11641 gcgaaaaggt tgccaaccat cctcaaccag taccgcaacc agaaagtagc ctagaccgtg
    11701 cgcgagccat ggaaatagct ttgaacgtgg cggatcgact ggtgaatatg ttcccgaatc
    11761 tgtcctcaga atcaaagcag gcagcagtag ctggagtgtt caacccgatt gttggtcgcg
    11821 atgttgtacc tctgccagct atctcagagc actactactc tgctacggaa gtcggccagc
    11881 gctacggtat ttcagccaac aaggtaggac gcatagccaa cacctacatg ctcaaaacag
    11941 aaaagtacgg gaagtggttc atcgacaaat cggcgcacaa tgacaagcag gttgaaacat
    12001 ttcggtacaa cgaggctgga gtgcacaaaa tagaggaact catcgaagga gggcgaaagg
    12061 ctgcatgatt ttgacaagat agttttcccc aaatgtgggg aaaagcccga atggcgcggc
    12121 ttacagcaag ataaggccta catgatttga caacaccgca ttaacggggc tatattccgc
    12181 ttcatggtgc tcaaaacacc tttgcgatag cggaaaccgc cccgtcagtt atgcggattt
    12241 ttttatgtct acgacattga gaattcaatg gtcgggcgtg cggctaataa aacacccgca
    12301 agggaaatat gcccgccgtc tatcgccggt tttgagcgcc cgaccaccct ctcaaaaggg
    12361 gtaaattcaa aatatgcgat aggacataaa accatggcta ctcaaatttc tgttgaatcc
    12421 ctttccccaa ttacctataa ccacatcccc gtcatcacta ccgaactact ggcacgactt
    12481 tacggcactg agaccaacaa cattaaagtg aactatacgc gcaatgccgg gcgttttgtt
    12541 tgtggtaagc actactttaa actggagggt ggtgaattgc gggaatttaa gaacaaggtt
    12601 actcacagta acttagttgc accgcgagca aagcacctca tcctctggac agaacgaggc
    12661 gcagcccgcc acgcaaaaat gctcgaaacc gatcaggcgt gggaagtgtt cgaaaaactg
    12721 gaagactgct atttcagccc gggcaaagcg gaacagcacc cgcagatcca gcagcaattc
    12781 acagacgaag aaatcatcct cctttgctat atgcaggtac agatggagaa tgcgctggac
    12841 atctgcaaac gtctgtaccc gatattgaag gaactgaact catcatacac gagcaagctg
    12901 tatgacatcg cgtttgaaac tttttacacg gtgacgaaaa acagagatgt actgctaagg
    12961 gaggcgacac gacttgacca agcaagcgcc cttttcgaac gggcaaaacc aatgctgaaa
    13021 agccttcggg cgagacaatt cgaattttaa tcatcaaagg agcttcggct ccttttttgt
    13081 tggagaaaat aaaccaatac tcgctccctt gcgagtaatt gcggagactt tgcgatgtac
    13141 ttgacacttc aggagtggaa cgcacgccag cgacgcccaa ggagccttga aacagttcgt
    13201 cgatgggtac gcgagtgcag gatattccct cctccggtta aggatggaag agagtatttg
    13261 ttccacgaat cagcggtaaa ggttgactta aatcgaccag taacaggtag ccttttgaag
    13321 aggatcagaa atgggaagaa ggcgaagtca tgagcgccgg gatttacccc ctaaccttta
    13381 tataagaaac aatggatatt actgctacag ggacccaagg acgggtaaag agtttggatt
    13441 aggccgagac aggcgaatcg caatcactga agctatacag gccaacattg agttattttc
    13501 aggacacaaa cacaagcctc tgacagcgag aatcaacagt gataattctg ttacgttaca
    13561 ttcatggctt gatcgctacg aaaaaatcct cgccagcaga ggaatcaagc agaagacact
    13621 cataaattac atgagcaaaa ttaaagcaat aaggaggggg ctgcctgatg ctccacttga
    13681 agacatcacc acaaaagaaa ttgcggcaat gctcaatgga tacatagacg agggcaaggc
    13741 ggcatcagcc aagttaatca gatcaacact gagcgatgca ttccgagagg caatagctga
    13801 aggccatata acaacaaacc cggtcgctgc cactcgcgca gcaaaatcag aggtaaggag
    13861 atcaagactt acggctgacg aatacctgaa aatttatcaa gcagcagaat catcaccatg
    13921 ttggctcaga cttgcaatgg aactggctgt tgttaccggg caacgagttg gtgatttatg
    13981 cgaaatgaag tggtctgata tcgtagatgg atatctttat gtcgagcaaa gcaaaacagg
    14041 cgtaaaaatt gccatcccta caacattgca tgttgatgct ctcgggatat caatgaagga
    14101 aacacttgat aaatgcaaag agattcttgg cggagaaacc ataattgcat ctactcgtcg
    14161 tgaaccgctt tcatccggca cagtatcaag gtattttatg cgcgcacgaa aagcatcagg
    14221 tctttccttc gaaggggatc cacctacctt tcacgagttg cgcagtttgt ctgcaagact
    14281 ctatgagaag cagataagcg ataagtttgc tcaacatctt ctcgggcaca agtcggacac
    14341 catggcatca cagtatcgtg atgacagagg cagggagtgg gacaaaattg aaatcaaata
    14401 atgattttat tttgactgat aatgacctgt tcgttgcaac aaattgataa gcaatgcttt
    14461 tttataatgc caacttagta taaaaaagca ggcttcaacg gattcatttt tctatttcat
    14521 agcccggagc aacctgtgaa cacattttca gtttcccgtc tggcgctggc attggctttt
    14581 ggcgtgacgc tgaccgcctg tagctcaaca ccacccgatc aacgtccttc tgatcaaact
    14641 gcgcctggta cctcttctcg cccgattctg tcggcaaaag aagcgctgaa tttcgatgcg
    14701 caacactatt ttgcatccct gacaccaggt gctgcagcgt ggaatccgtc cccgataacc
    14761 ctgcctgcgc aacctgactt tgttgtcggc ccggcgggta ctcaaggtgt aacgcatacc
    14821 acgattcagg cggcggtaga tgcggcaatt atcaagcgca ccaacaagcg ccagtatatt
    14881 gccgtgatgc ctggtgagta tcagggaacg gtgtatgtcc ctgctgctcc aggtggaatc
    14941 actctgtacg gtacaggtga aaaaccgatt gatgtgaaga ttgggctttc ccttgatggg
    15001 ggcatgagcc ctgccgactg gcgtcacgac gtcaacccgc gcggcaaata tatgccaggt
    15061 aaaccagcgt ggtatatgta cgatagctgc cagagcaaac gcagcgacag tatcggtgtt
    15121 ctgtgttctg cggtcttctg gtcacaaaac aatggactgc aactgcaaaa tctgaccatc
    15181 gaaaacacgc tgggcgatag cgtagatgca ggtaaccatc cggcggtagc actgcgtact
    15241 gacggtgacc aggtacagat taacaacgtt aacattctcg gtcgtcagaa caccttcttt
    15301 gtcaccaaca gcggtgtgca gaaccgtctg gaaaccaacc gtcagccgcg tacgctggtg
    15361 accaacagct atattgaagg ggatgtggat atcgtttctg gtcgcggcgc agtggtgttc
    15421 gataacaccg aattccgcgt ggtgaactca cgtacccagc aagaagcata tgtgtttgca
    15481 ccggctacgc tgtctaacat ctattacggt ttcctcgccg taaacagccg tttcaatgct
    15541 tccggtgatg gcgtggcgca actgggccgc tcgctggatg ttgatgctaa taccaacggt
    15601 caggtggtga tccgtgatag cgccatcaac gaaggtttta acacggctaa accgtgggcc
    15661 gatgcggtga tctctaatcg tccgtttgcg ggtaataccg gcagcgtaga tgataacgac
    15721 gaaatacagc gcaatctgaa tgacactaac tacaaccgca tgtgggaata caataaccgc
    15781 ggcgtgggca gcaaagtggt tgcagaggcg aagaagtaag agctattaac tatttgccgg
    15841 atgcggcgta aacgccttat ccggcctacg gttcgatgcg atttgtaggt cggataagat
    15901 gcgccagcat cgcatccgac aataagtgcc gaatgcgacc tacattcaca tggcgctttt
    15961 tacatctgac ggtttttatt gaagttaatc aaactacccg ccttgataat ctcgcgctct
    16021 tcagccgtca ggctttccat gtaaagcgta atttccgtta ccggcgcatc ttcatggatc
    16081 acataacctt taaacgtcgt acccggatta tccagcgccg ctttaatgcc agggatgtaa
    16141 atgtaatccc ccacttcaaa ggttggcact tccgccatct gcagcggtaa catcccccag
    16201 ttgatgacgt tagaacggta gcgtttagtc gcgtactcct cggcaatatt cgccagaccg
    16261 ccaatcacac gctggcagct cgccgcctgt tcacgcgcag aaccatcgcc tggtttcacc
    16321 gcatagacca tgctgccaat ttcagtttgc agcggatcaa tatgctcctg accagcaatc
    16381 tgcttaatgc gcgcaaacac ctctgtcagc tcgctgacat tccccgccag accaatcgga
    16441 tttgaacgat aagaagaggt ttcaccggaa ggaatcagtt cgtcggtggt ggtgacttcg
    16501 tcgaggatct tcgagcacac tttcaggacg atattgtcag tcagcgcacc caattccggc
    16561 cagtctttaa tgttcggtcc gtaaatcagc ggttgctgag ttgccccttt cacaaagccc
    16621 tgataaacac ggtttttata cggcgttaca tcgaaggcgt actccggcac gttgtcccag
    16681 caatcaagtt cgctggcaga ggttaaatag ccaccgtttg ccgcagtcgc agcgatagaa
    16741 cgagcgtcca tcaacgccac cgctgacatc tgcccattgg ctggcttaga gccttcgcgg
    16801 ttcgggaagt tacgcgtggt gtggcgaata ctcaaaccgt tgttgattgg cgtatcgccc
    16861 gcgccaaagc atgggccaca gaatgcggtt ctgatgattg cgcctgcgcc aatcaaatct
    16921 gctaccacac cttttttggc gagatccata aacaccggct gtgatgacgg gtaaaccgcc
    16981 agcgagaagg tgtcattgcc acaggattga ccgcgcagtg cattcgccgc cgcgatgacg
    17041 ttttcgtagt taccgccaga acagcccgcg ataatcccct gctgcacttt caggcgacca
    17101 ttttccactt tatccagcag cgagagtttg gctttaccgt gcgccacgcg ttcggactca
    17161 atttcaatct cacgcagaat gtcggtcagg ttctggttca gtgtgtcgat ttcatacacg
    17221 ttgctcgggt ggaacggcag cgcaatcatt ggtttgatgg cgcttaaatc aacgctgatg
    17281 cagccatcgt agtacgccat cggttgaggg ttaagctggc agtaatcctg gccgcgaccg
    17341 tgcagcgcca gccagttatg cacttcttca tcagtttgcc agacggagct taaacaggtg
    17401 gtttccgtgg tcatcacgtc aacgctgtta cggaaatcgg tagagagcgc gctaacgccc
    17461 ggtccaacga actccatgac tttgtttttg acgtaaccgt ttttgaacac cgcgccaatg
    17521 atagccagcg ccacatcctg tggccccaca tacggcgcag gttttccggt cagatgcacc
    17581 gcaaccacgc ccggatagtc gatatcccag gtgtcattaa gcagctgttt taccaactca
    17641 ccgccaccct caccgactgc cattgtccct aatgcaccgt aacgggtgtg gctgtctgac
    17701 ccgaggatca ttttgccccc gcctgccatc atctcacgca tatattgatg gatgaccgca
    17761 atatgcggag gcacaaaaat accgccataa cgctgagctg ccgataaacc aaaaacatgg
    17821 tcatcaccgt taatggtgcc gccgacggcg cagagtgagt tatggcagtt ggtcagcaca
    17881 tacggcagcg ggaaacgttc cattccggac gctttagcgg tctgtacaat accaacaaag
    17941 gtaatatcgt gagaggctaa tgaatcaaac ttaattttaa gtttatccat atttccggac
    18001 gtattatgag aagagagaat agaccaggca atagtgcctt tttttgcttc ttctttttta
    18061 atttcgccgg tgaaatgttc ttcggcaatt atttcgttat tactggcgag aaacacgcct
    18121 ttttcagata acttgatcat caaatgctcc agatatctgc gcaccgtgaa gtgcgcagaa
    18181 agataataaa tggatatact ctaaataatt cgagttgcag gaaggcgaca agtgagtgaa
    18241 tccccaagag cttacataag taagtgactg gggtgagcga acgcagacgc agcacatgca
    18301 acttgaagta tgacgagtat atttacagca tgttccagcc gatcagcata ttccaccacc
    18361 acacgccgag ggtgatatgc accaggaagg ttaatatcgt cagtaccgca ccgaccaacc
    18421 atcaggattt aatatcgttg taacccacgc caaagatgac cggacctgcc gcgccgccat
    18481 agtgagtaac catgccgcca taggagttgg agaacaacag tgccagcgcg gttaacatta
    18541 acggtgcgcc ggagacgttc gccagcatgg caaatacagg taacatagca acgatatagg
    18601 cactaccgga agcgaagaaa taacgcacga tgatgctgag gaaaataata acgaagaaag
    18661 caacgttacc gtgaccatca aatgccaggt tatttttaaa gacttcagct aaccattcga
    18721 aaaatttaac tttcgataat aaggagctta agccgataat accgccgtac cagattaagg
    18781 tattccagcc gcctttattt ttaaccacgt cttcccaggt aacgataccc agcagcagca
    18841 tggtcgccat aacaacgatt gccacagtgg attcatcaac ccccagagac ttactgaaaa
    18901 tccagcccag tagcgccagc acaaagacac cgagcagcat tttttcgcgg attttcatcg
    18961 gtcccagttc tgcaaggccc gctttagcga tggttttgtt atccaccttt ttaatttctg
    19021 gtggatacat ggtgtaaatt accagcgggg tgaccagcag cataatgatg cccggcaatc
    19081 cggcggccag cgcccatcca ccccagctaa tttgcaggtg cagaatgtcg ttgatcattt
    19141 tcagcgccag aatgttcccc tccattgcgg taaagaacat atagctggtg gttttggtga
    19201 ccatgtaaat ggacatcatc aggtaatgtc cgacacgacg cggacttttt tccggttcgg
    19261 accccaaagc caccgccacg ctgttgatga tcggtaacac aatgccgccc gcacgcgcgg
    19321 tgttagacgg tgttgccgga gccagtacca gatcgaggaa taccgtaacg taacccagac
    19381 ccagcgtggt gttaccgatt ttaccaatca gcagataggc aatacgttta cctaaaccgg
    19441 tggtcacaaa tgcggcgctt aaggtaaacg ccgagaacac cagccaggta gtacctgaag
    19501 agtaaccgct taatacggcg gtggtcttaa acgcaccgtc ggataagtta ccgaccacca
    19561 ccattgaggc agcaacggca attaacagta cgacaggttc cgggaaaggc ttgataacca
    19621 gacccacaat ggccgccagg taaataccaa aaagcaccca cgccagttcg cttaatcctg
    19681 ccggagctgg cataaaacca ataatacatg ggatcgctaa tatcagaatt agcttccata
    19741 acgatttctt attcatttaa aatcctgttc tttctgtatg actcatacag aagtccttta
    19801 ttgattgtcc ttatcctgac aacgaatttt ttcagggaag ataaacttca ccggaaaata
    19861 tttttctggt tgtccgaata acagatgcgc gtaacgtcgt ggcatcctga ccttcattac
    19921 ttaaatgaac gtcgagcgca ccactggggt gttcaatatt gatattgcca tatcctacag
    19981 aagggacgat ttgtcgggtg acggtgcctt ccaatgcaca actactggaa atagcaatag
    20041 caccggttat cgccagcgcg cgatggcaag aatgcggcat aaaataacgc acattaattg
    20101 ccccgccttt ctgcgctgga gaaataagca caggtttagg gataaccata ttactgacat
    20161 cacctaagcc cattgcttta cccgcttgta gccggataga ttcaatgcgg gctaataatg
    20221 ctttgtcggc atccagttcc gccggtaatt cataacctgt ttttcccaga tattcagccg
    20281 gaataatgac gactggcatc gccatatcga tacaggtcac cgggacatcg tcaaaataat
    20341 caatctgatt atcagtcggg aaaacttttc cggttttcgt tccagcggca ttcaggaaag
    20401 tgagcgcaac cggtgcggca gtacccggta cgccgtcaat tctggcgcta ccctcgtact
    20461 cgacaacacc atttggcgtt tgcacatcag cttcgatgaa cgtacccgta ttgacgttgc
    20521 ggatacgtac acgggtaact ggcgaagtcg ctgcaatcaa accattttca atggcgaatg
    20581 cccctacgcc agacagcata ttgccgcagt taggcgtggt atcgacacgt tgctcatgga
    20641 cgataacttg agcaaacaga taatcgacat cagcacgcgg atcgctggaa cggctaataa
    20701 tggcgacttt actggtcagt ggattaccgc cgccaatacc gtcaatttcc agatcgttac
    20761 cggaacccat aattgccatc aatattttat cgcgctgcgt ttgatcttcg ggtaaatgtt
    20821 ccgctaacag gaacgcgccc ctcgaggttc cacctcgcat catcacgcag ggtatttttt
    20881 tcataataat gccctcaatt taaaaacata acttattgcg atatggttac attaaggaca
    20941 aagcatctct aatgacttta gctcaacgat taataacatt ttttcattaa tttaagttgg
    21001 ctaaatgcag ttaaaatttt taacgaccag ccacccaa
//
LOCUS       DADLSA010000059        20991 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.059, whole genome shotgun sequence.
ACCESSION   DADLSA010000059 DADLSA010000000
VERSION     DADLSA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.059"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..333)
                     /locus_tag="JN691_002207"
     CDS             complement(<1..333)
                     /locus_tag="JN691_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7243650.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRV"
     gene            complement(511..1650)
                     /gene="actP"
                     /locus_tag="JN691_002208"
     CDS             complement(511..1650)
                     /gene="actP"
                     /locus_tag="JN691_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAY7243651.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALSLGLGLM
                     FGTAGLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYK
                     DAAGHLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLY
                     ANVFKKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPI
                     ILLSMYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSI
                     SVAFLGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1647..1961)
                     /locus_tag="JN691_002209"
     CDS             complement(1647..1961)
                     /locus_tag="JN691_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAY7243652.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2161..4119)
                     /gene="acs"
                     /locus_tag="JN691_002210"
     CDS             complement(2161..4119)
                     /gene="acs"
                     /locus_tag="JN691_002210"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HAY7243653.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4512..5948
                     /gene="nrfA"
                     /locus_tag="JN691_002211"
     CDS             4512..5948
                     /gene="nrfA"
                     /locus_tag="JN691_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAY7243654.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5993..6559
                     /gene="nrfB"
                     /locus_tag="JN691_002212"
     CDS             5993..6559
                     /gene="nrfB"
                     /locus_tag="JN691_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAY7243655.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6556..7227
                     /gene="nrfC"
                     /locus_tag="JN691_002213"
     CDS             6556..7227
                     /gene="nrfC"
                     /locus_tag="JN691_002213"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAY7243656.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7224..8180
                     /gene="nrfD"
                     /locus_tag="JN691_002214"
     CDS             7224..8180
                     /gene="nrfD"
                     /locus_tag="JN691_002214"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAY7243657.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8200..9918
                     /locus_tag="JN691_002215"
     CDS             8200..9918
                     /locus_tag="JN691_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY7243658.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9911..10294
                     /gene="nrfF"
                     /locus_tag="JN691_002216"
     CDS             9911..10294
                     /gene="nrfF"
                     /locus_tag="JN691_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAY7243659.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10291..10887
                     /gene="nrfG"
                     /locus_tag="JN691_002217"
     CDS             10291..10887
                     /gene="nrfG"
                     /locus_tag="JN691_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAY7243660.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11229..12542
                     /gene="gltP"
                     /locus_tag="JN691_002218"
     CDS             11229..12542
                     /gene="gltP"
                     /locus_tag="JN691_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAY7243661.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12620..13309)
                     /locus_tag="JN691_002219"
     CDS             complement(12620..13309)
                     /locus_tag="JN691_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7243662.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13403..15550)
                     /gene="fdhF"
                     /locus_tag="JN691_002220"
                     /pseudo
     CDS             complement(13403..15550)
                     /gene="fdhF"
                     /locus_tag="JN691_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(15748..17214)
                     /gene="mdtP"
                     /locus_tag="JN691_002221"
     CDS             complement(15748..17214)
                     /gene="mdtP"
                     /locus_tag="JN691_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAY7243663.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTHLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17211..19262)
                     /gene="mdtO"
                     /locus_tag="JN691_002222"
     CDS             complement(17211..19262)
                     /gene="mdtO"
                     /locus_tag="JN691_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HAY7243664.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19262..20284)
                     /gene="mdtN"
                     /locus_tag="JN691_002223"
     CDS             complement(19262..20284)
                     /gene="mdtN"
                     /locus_tag="JN691_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAY7243665.1"
                     /translation="MESTPKKAPRNKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGRIIS
                     LKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEG
                     KVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIIVDKPDPEMFRIGASAV
                     ANLEPQ"
     gene            complement(20303..20578)
                     /locus_tag="JN691_002224"
     CDS             complement(20303..20578)
                     /locus_tag="JN691_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243666.1"
                     /translation="MPTVLSRMAMHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(20787..>20991)
                     /locus_tag="JN691_002225"
     CDS             complement(20787..>20991)
                     /locus_tag="JN691_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallo-beta-lactamase"
                     /protein_id="HAY7243667.1"
                     /translation="RKTLQPQVDASFYISREDLHAVLTGQAKMADLVKAKRAKIIGNG
                     AKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         4719 a         5476 c         5698 g         5098 t
ORIGIN      
        1 aactcttctc gtaattctat cgtcacattt attatttgca tataacgcta tatctataaa
       61 atatttatgt attttcctgt ccgtttcttt cgtgattttt cctgatggta aactatcata
      121 aattcttttc attttctgaa tcaagcctat cactttttca tgatcatcta ttttgaaaag
      181 agagagggct ttattgtcaa ttaacgtgtt tttatgatgt ttgctgtaat tatctgattt
      241 tgttttcttt gtatgtgatg aacttgtgat agagtggtta tcgttaatct ggcaaagagg
      301 gaaaaacata ttatttaaac cattatagcg cataaaatat ccttataatt aacaagaaag
      361 aaaaggcata ttctctgcat tatcattttc tacgactgtc aaaaatcgct cattttttaa
      421 tgagtttatt tgtttaatat tatgggaaaa ggtgatgcat gttggagagg aagagtattc
      481 cccggtcagg cgaccgggga aggggagaga ttaatgtgcg cggccttgct caacgccaaa
      541 gccggtctgg gagcggataa actgcgcgcg gaacagttca cgctcgcgcg ccccttccgc
      601 tgagttatcg gttgccgaga agaaccagat gccgaggaat gccacgctga tagagaacag
      661 cgccgggtat tcatacggga agatggcttt ttcgtgaccg aggatctgta cccaaatagt
      721 cgggccgagg atcatcagca ccactgcggt aatcagcccc agccagccgc ccagcatcgc
      781 gccacgggta gtcagtttcg accagtacat ggaaagcaga atgatcggga agttacagct
      841 cgccgcgatg gcaaacgcaa ggcctaccat aaaggcgatg ttctgattct caaacagcac
      901 gccgaggata atcgcaatca cgccgaggat cagtacggtg atttttgata cccgcagctc
      961 ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac aagtcatgcg aaaccgccga
     1021 tgcgcccgcc agcgtcagac ccgcaaccac cgcgaggata gtggcgaaag caaccgctga
     1081 aataaaaccg aggaataggt tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt
     1141 gttaccacca atcagatggc ccgccgcgtc tttatattcc ggattcgcac caacaagcat
     1201 gatcgcgccg aagccgataa taaaggtcag aatatagaag tagcccataa atccggtggc
     1261 gtagaacacg ctcttacgtg cttcgcgggc atcgctgact gtaaagaagc gcatcagaat
     1321 gtgcggcaag cccgccgtac caaacatcag tcccagcccc agagagagcg cggagatccc
     1381 caagaatgag gcggcggaca tatagtcccc ggcaatcgcc agcccgttct ggaagccagt
     1441 gatattgccg cctgcggtgt agtagtcgct acgagaacgt acgcgttttg atgcccagta
     1501 ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct gccagttcgt
     1561 tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga aagggagtgt
     1621 ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag gacttcatta
     1681 ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccggtgag aacaaaggag
     1741 atcacaatca ctccaacacc aattggaata ccgcgtgtga cgctggtgtt cggattcagc
     1801 ggcgtgccca gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc
     1861 agcataataa tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa
     1921 tgcgcattgt cttctatccg ctgataaatc gtaccattca tcacagattc tccagaggta
     1981 atgtagggat tgttttaatt cccgctccct tatgggagaa ggttaacgct cgggtaaccc
     2041 ttgccgaatg taggccggat aaggcgttta cgccgcatcc ggcaatcaat gcctgatgcg
     2101 acgctgtcgc gtcttatcag gcctacaaac cgttaccgat tcgcatccgg caattgtggg
     2161 ttacgatggc atcgcgatag cctgcttctc ttcaagcagc ttctcgacca cgccaggatc
     2221 ggcaagcgtc gaggtatcgc ccaggttgct ggtatcgccc gccgcaattt tgcgcagaat
     2281 acggcgcata attttgccgg agcgggtttt aggcagggag tcggtccagt gcagcacgtc
     2341 tggcgtcgcc agcgggccaa tctctttacg cacccagttg cggacttctg cgtacagttc
     2401 tggtgacggt tcctccccgt gattaagcgt gacataggcg tagatcgcct gacctttaat
     2461 attgtgcgga atacctacta cggcggcttc ggcaatcttc ggatgcgcca ccagcgccga
     2521 ctctatctct gccgtcccca gacggtgacc ggagacgttc agcacgtcgt ccacacgccc
     2581 ggtgatccag taatagccat cttcatcgcg acgcgcgccg tcgccgctga aatacatatt
     2641 tttgaaggtg gaaaaatagg tctgctcaaa acgttcgtga tcgccaaaca gcgtacgcgc
     2701 ctgaccaggc caggagtcgg tgatcaccag gctaccttcg gtagcccctt ccagcgggtt
     2761 accttcgtta tcgaccagcg ccggttgcac gccgaagaac ggacgtgttg ccgaaccggc
     2821 tttcagctcg gtagcgccag gcagcggggt gatcatgaaa ccgccggttt cggtctgcca
     2881 ccaggtatcg accaccggac atttctcgtt gccgattttt ttccagtacc actcccacgc
     2941 ttccgggtta attggctcgc ccacggaacc gagaatgcgt agcgacgaac ggtcggtgcc
     3001 ttcaatcgct ttatcgcctt ccgccatcag cgcgcggatc gccgtgggtg cggtatagag
     3061 aatattgacc tgatgcttgt ccaccacctg cgccatacgg gcaggcgtcg gccagttcgg
     3121 tacgccttca aacatcagcg tggtcgcgcc gcaggccagc gggccgtaca gcaaataact
     3181 gtgtccggta acccagccca catcggcggt gcaccagtag atatcacccg gatgataatc
     3241 aaagacatat ttaaaggtca gcgccgcgta caccagataa ccgccggtag tgtgcagtac
     3301 gccttttggt tttccggtag aaccggaggt atagagaata aacagcggat cttcggcgtt
     3361 catctcttcc gcctggtgct gatcgctggc ttgctcaacc aggtcgtgcc accacaggtc
     3421 gcgcccttcc tgccagtcga tgtttccgcc agtacgcttc agtaccacca catgctctac
     3481 gctggtgacg ttcgggtttt ttagtgcgtc atcaacgttt ttcttcagcg gaatactacg
     3541 cccggcgcgc acgccttcgt cggaagtgat caccagtcgt gagttggaat caataatgcg
     3601 cccggcaacg gcttccggcg agaagccgcc gaaaatcacc gaatgcaccg cgccaatgcg
     3661 ggcgcaggcc agcatcgcaa ccgcggcttc cggcaccatc ggcatataaa tcgccaccac
     3721 atcacctttt ttaatgccca gctcgagcag ggtattggcg aagcggcaga cgtcgcggtg
     3781 cagctcttta tagctgatat gtttgctctg gctggcgtcg tcgccttccc agatgatggc
     3841 ggtgcgatcg ccgttttctt gcagatggcg gtcaaggcag tttgccgcca gattcagcgt
     3901 gccgtcctcg taccatttaa tggacacatt accgggggca aaggaggtat ttttcacctt
     3961 ctggtacggt ttgatccagt cgagaatttt tccctgttcg ccccagaagg tatcaggtac
     4021 gttaatagat tgttgataca tcgcctcgta ctgctgaggg tttatcaggc aacggtctgc
     4081 gatgttggca ggaatggtgt gtttgtgaat ttggctcatg cttttgttct ccttgtagga
     4141 tgttaataat atgtggcata agcgttaaat gtaggggtat tggcagtttt gtttagtatt
     4201 tgggcgacag atcacgcaaa agtagaatgg tgcaaataaa cggcagggta atttttgaag
     4261 gtcaccaaca aaagttgatt aattctttga ggaacatgca gttatgcatg ctgttgaaaa
     4321 agaggaagat actgactaac tctaaagtgg tattttacat gcacttacaa ttgattaaag
     4381 acaacattca cagtgtggtt atttgttaca cataggggcg agcaatgtca tgacagtgta
     4441 ggtgcggtta ctgtcgtgaa aaacaataaa aaccgccatt gcaacaatgg cgcaattcgg
     4501 atgaagcccc tatgacaagg ataaaaataa acgcacgccg tatcttcagc ttattgattc
     4561 cttttttctt tttcacttct gttcacgctg aacaaacggc agctcccgca aaacctgtaa
     4621 ctgtggaagc gaagaatgaa acctttgccc cgcagcatca cgatcaatat ctctcctgga
     4681 aagccacctc ggaacagtca gagcgtgttg acgccctggc ggaagatcca cggctggtga
     4741 tcctgtgggc ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga catgcctttg
     4801 ctgtgaccga tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct gaagatggtc
     4861 cgctaccgat ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg atccagaaag
     4921 acggcgaaga tggctacttc cacggtaaat gggcgcgcgg cggcccggaa atcgtcaaca
     4981 acttaggttg tgccgactgc cataacaccg cctcaccaga gttcgccaaa ggcaaaccgg
     5041 agttaaccct ttcccgtccg tatgcggctc gcgcgatgga agccattggt aaaccttttg
     5101 agaaagccgg acgtttcgac cagcaatcga tggtttgcgg tcagtgccat gtggagtatt
     5161 acttcgacgg caaaaacaaa gcggttaaat tcccgtggga tgacggcatg aaagtcgaaa
     5221 atatggagca gtattacgac aaaattgcct tctctgactg gactaactcc ctgtcgaaaa
     5281 cgccaatgct gaaagcgcag cacccggaat atgaaacctg gacagcgggc attcacggta
     5341 aaaacaacgt gacctgtatc gactgccata tgccaaaagt gcagaacgcc gaaggcaaac
     5401 tctacaccga ccataaaatt ggtaatccgt ttgataactt cgcccagact tgtgcgaact
     5461 gccataccca ggataaagct gccttgcaaa aagtggtcgc ggaacgtaag caatcgatta
     5521 acgacctgaa aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa gcgaaagcgg
     5581 cgctggatgc aggcgcgacg gaagccgaaa tgaagccgat tcaggacgat atccgtcatg
     5641 cccagtggcg ttgggatctg gcgatcgctt cccacggtat tcatatgcac gcaccggaag
     5701 aaggtctacg gatgctcggt acggcgatgg ataaagcggc ggatgcgcgc accaaactgg
     5761 cgcgcctgct ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat atctcgacca
     5821 aagagaaagc ccagcaggcc attggcctga acatggaaca aatcaaggcc gagaagcagg
     5881 acttcatcaa aacggtgatc ccgcagtggg aagagcaggc acgtaaaaac ggtctgttaa
     5941 gccaataacc ccgttccgcc tcgcaagggg cggaaaacac aatggagtga atatgagcgt
     6001 attacgttcg ttattaactg ctggggtgct ggcgtcgggc ctgttgtgga gcctgaacgg
     6061 aattaccgct acccctgcgg tgcaggcatc tggtgatcgc tacgaagtta cccagcagcg
     6121 taacccggat gccgcctgtc tggactgtca taaaccagat accgaaggta tgcatggcaa
     6181 acatgcttcc gttatcaacc cgaataacaa attgccggtc acctgcacca actgtcacgg
     6241 ccagccatca ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta acgagccgat
     6301 gtacaaggtc ggagagcaga acagcgtctg tatgtcctgt catctgccgg aacagttgca
     6361 aaaagcgttc tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca gctgccattc
     6421 cctgcatcca cagcaagata cgatgcagac gttaagtgac aaaggacgga ttaagatttg
     6481 cgtcgattgc cacagcgatc agcgcaccaa tccgaacttt aacccggcgt ctgttccgtt
     6541 gcttaaggag cagccatgac ctggtctcgt cgccagtttc tcaccggcgt cggcgtgctg
     6601 gcagccgtca gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat caatggggtg
     6661 cgttacggca tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg tatggatgct
     6721 tgtcgggaag tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat tcgtagcgag
     6781 ccgcagggcg aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg ccagcactgc
     6841 gatcatgcgc cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga tgccgctagc
     6901 gggattgtcg atgttaatcc tgatctctgc gtcggttgtc agtactgcat cgccgcctgt
     6961 ccgtaccgcg tgcgctttat ccatccggtc acgaagacgg cggacaaatg cgatttctgt
     7021 cgtaagacca atttgcaggc cggtaagctt cccgcgtgcg ttgaagcttg cccgacgaag
     7081 gcgctgacgt ttggcaatct ggacgatcct aacagtgaga tttcgcaact gctgcgccag
     7141 aagcctactt accgctacaa gctggcgctg ggaaccaaac cgaagctgta ccgcgtaccg
     7201 tttaaatacg gggaggtgag ccaatgacgc agacttccgc atttcatttt gaatcgctgg
     7261 tgtgggactg gccgattgcc atctacctgt ttttgattgg tatttctgcc ggtctggtga
     7321 cgctggccgt gctgttacgt cgcttctacc cgcaggcggg cggtgcagac agtacgttgc
     7381 tgcgcaccac gctgattgtc gggccgggcg cggtgatcct cggtctgttg atcctcgtct
     7441 tccacctgac aagaccgtgg accttctgga agctgatgtt ccactacagt tttacctcgg
     7501 tgatgtcgat gggggtgatg ctgtttcagc tctacatggt ggtgctggtg ctgtggctgg
     7561 cgaaaatctt tgaacatgat ttgcttgccc tgcaacaacg ctggttgccg aagctgggga
     7621 tcgtgcaaaa ggttctgagc ctgctgacgc ccgttcatcg cggactggaa acgctgatgc
     7681 tggtgttggc ggtgctgctg ggggcttata ccggctttct gctgtcggcg ctgaaatcgt
     7741 atccgttcct caataacccg agcctgccgg tgctgctcct cttctccggc atctcgtccg
     7801 gtgcggcggt ggcgctgatc gccatggcga tacgccaacg cagtaacccg cattccacgg
     7861 aagcgcactt tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc ttcctgctgg
     7921 tggcgttttt tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg ctggtggcgg
     7981 cattaggtgg cggtttctgg acgtggtggt tctggcttgg tgtcgccggg ctggggctga
     8041 ttgtgccaat gttgctcaaa ccgtgggtca atcgcagttc cggcattcct gccgtgctgg
     8101 cggcgtgtgg ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt ctctacgccg
     8161 gacagttgac ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt ccttcctgaa
     8221 gtcggttttc tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc gttgacggcc
     8281 tttgcgggag tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat cggcattctg
     8341 gcgcagttcg cgctcctgct gctcgccttt ggcgtactga cgtattgttt tctcatcagc
     8401 gatttctcgg tcatttatgt cgcccaacat agctacagcc tgctgtcgtg ggaactcaaa
     8461 ctggcggcgg tgtggggcgg tcatgaaggt tcgctgctgc tttgggtgct gctgctttcc
     8521 gcctggagcg cgctgtttgc ctggcattat cggcagcaaa gcgatccgct atttccgctg
     8581 acgctagccg ttttatctct catgctcgcc gcactgctac tgtttgtggt gctgtggtcc
     8641 gatcccttcg tgcggatatt tccaccagca atcgaaggcc gcgatctcaa tccgatgctg
     8701 caacatcccg gtcttatctt tcatccaccg ctgctttacc ttggctatgg cggtttgatg
     8761 gtagcggcga gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc ggccagcgcc
     8821 cgaatttgct ggcgctgggc gttacctggc tggagtgcat taacggcggg gatcatcctc
     8881 ggttcctggt gggcctactg cgaactcggc tggggcggct ggtggttctg ggatccggtg
     8941 gaaaacgcct ctttattacc ctggctttct gccactgcgc tgctgcacag tttgtccctg
     9001 acacgccagc gggggatttt ccgccactgg tcgctgttgc tggcgatagt tactctgatg
     9061 ctgtcgctgc tgggcacctt aattgtccgt tctggcattc tggtttcggt tcatgcgttc
     9121 gcactggata acgtccgcgc tgtgccgttg ttcagcctgt ttgcactgat tagccttgcg
     9181 tctttggctc tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg tttttcgggg
     9241 ttatcgcggg aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt gctactgatc
     9301 gtgctggtgg gaacgcttta tccgatgatt tacggcctgc tgggctgggg acgcctctcc
     9361 gttggcgcgc cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat gctggtggtg
     9421 attgtgctgg cgacgtttgt ctctggcaaa cgcgtgcagc ttccggcgct ggtagctcat
     9481 gctggcgtgc tgttatttgc cgcggggatc gtggtttcca gtgtcagtcg tcaggagatc
     9541 agcctgaatt tacagccggg tcagcaggtg acgctggcag gatacacctt ccgttttgag
     9601 cgactcgatc tgcaagccag aggcaattac accagcgaaa aagcgatagt ggcgctgttt
     9661 gaccatcagc aacgcattgg tgaattaacg ccggagcggc gtttttatga agcacgccgt
     9721 cagcaaatga tggaaccgtc aattcgctgg aacggcatcc atgactggta tgcggtcatg
     9781 ggtgaaaaaa ccagagcgga tcgttacgct tttcgtttgt atgtacaaag cggtgtgcgc
     9841 tggatctggg ggggaggatt gttgatgatt gcgggcgcat tgttaagcgg atggcggggg
     9901 aagaagcgcg atgaataaag ggcttctcac gctactgctg ttatttacct gttttgcgcg
     9961 cgctcaggtt gtagacacct ggcaattcgc caatccgcaa caacagcaac aggcgttaaa
    10021 tattgccagc cagttacgtt gtccgcagtg ccagaatcaa aacttactcg aatccaatgc
    10081 gccggtggcg gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg ggaagaacga
    10141 agtcgaaatc attggctgga tgaccgaacg ctacggagat tttgttcgct ataacccacc
    10201 gttaacgggt cagacgctgg tgttgtgggc gctgccagtg gtgttgttat tgctgatggc
    10261 actgatcctc tggcgagtga gggctaagcg atgaaacagc ctcaaatacc ggtgaaaatg
    10321 ctgacaacac tcacgatatt gatggtattc ctctgtgtcg gcagttatct gttaagccca
    10381 aaatggcagg cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca tcagtttgcc
    10441 agccagcaaa ccccggaagc gcagcttcag gcattgcagg ataaaatccg cgctaatcca
    10501 caaaacagcg aacaatgggc gttactgggc gagtactatc tgtggcaaaa cgattacagc
    10561 aattcgctgc tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc tgaactgtat
    10621 gcggcgctgg cgacggtgct ttattaccag gccagccagc atatgaccgc ccagactcgc
    10681 gcaatgatcg acaaagccct cgcgctggac tctaacgaaa tcaccgctct gatgctgctg
    10741 gcttccgatg cgtttatgca ggcgaattac gcgcaagcca tcgaactatg gcaaaaagtg
    10801 atggatctca actcaccccg gatcaaccgg acacagctgg tcgagtcgat taatatggca
    10861 aaactgttgc agcggagatc ggattaatca tctggtgatg aagattatgt cattgagctg
    10921 cataaaaaat aatcgaatga acatatgcca aaaataatca ccaatcagta ttattgcaga
    10981 ttaaacaaat aaaaatcttt ccataacaaa tggttattca ttaatcctgc cataaactcg
    11041 cgttattatg cattaatgca gcgaaaagct ctgttgttaa agggttgcgc aacataccgc
    11101 gcaaatgata ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac acaattcatg
    11161 ccctggcagt atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc attgaggaag
    11221 ttattctgat gaaaaatata aaattcagcc tggcctggca gattctgttt gctatggtgc
    11281 tgggcattct cctgggaagc tacctgcact accatagcga cagccgcgac tggctggtcg
    11341 tcaatttgct ctctccggcg ggtgatatct tcatccatct gatcaaaatg attgttgtgc
    11401 cgattgtgat ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc aaacagctcg
    11461 ggcgtattgg cgcgaaaacc attatttact tcgaggtgat caccaccgtc gccatcattt
    11521 tggggatcac tctggcgaac gtcttccagc ccggtgccgg ggtggatatg tcgcagctgg
    11581 cgaccgtcga tatctcgaaa tatcagagca ctacggaagc ggtacaaagc agttcccacg
    11641 gcattatggg cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg atggcgaaag
    11701 gcgaaatgct gccgatcatc tttttctcgg tgctgtttgg tctggggctt tcttccctgc
    11761 ccgcgacgca tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa accatgttta
    11821 aagtgactca catggtgatg cgttatgcac cggtgggtgt gtttgcgctg attgcggtga
    11881 cggtggctaa ctttggtttc tcgtctctgt ggccactggc gaaactggtg ctgctggtgc
    11941 atttcgccat tctgttcttc gcgctggtag tgctgggaat tgtggcacgt ctgtgcgggt
    12001 taagcgtctg gatcctgatt cgtattctga aagatgagct gattctggcg tactccactg
    12061 ccagctctga aagcgtactg ccgcgaatta ttgagaagat ggaagcctac ggagcaccgg
    12121 cgtcgatcac cagcttcgtg gtgccgaccg gttactcttt taaccttgat ggttcgacgc
    12181 tgtatcaaag tattgccgct attttcatcg ctcagctgta tggcattgac ctgtccatct
    12241 ggcaggaaat cattctggtg ctgacgctga tggtgacctc gaaagggatt gctggcgtgg
    12301 ctggcgtgtc gtttgtggtg ttgctggcaa cgctgggtag cgtaggtatt ccgctggaag
    12361 gtctggcgtt tattgctggt gttgaccgta tcctcgacat ggcgcgtact gcgctgaacg
    12421 tggtgggtaa tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa tttgaccgca
    12481 aaaaggcgct ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact gcggatcaat
    12541 aattgaagat tgccggggat atcccccggc aatgtgtgga tgcctgatgc gacgcttgcc
    12601 gcgtcttatc aggcctacct taaccgttgg ttaatttttc aaactcttca cacccggtat
    12661 caaacccttc catacagctc aggttcaacc agtgcagcgc cttttgtttg ttcttctcga
    12721 taaaaccttc ctcaccgttt aagaacatca ttcccgccca gtactcggaa taaccggttc
    12781 gggaaattgc agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc
    12841 ccacgccgtt ggcgtaaatc aaaccaagca gcatttgggc atcgacggcg gagtcattct
    12901 ccagatcttc cgaggcgttt tctaacagcg aaatcgcctt tggataatcc ggtttacccg
    12961 cttgagtatt taccagaata tgcgccagcg ttacttcacc ttctttactg cccgcttgcg
    13021 ccgctttctc tgcaagaact tttgcttgtg gatagtccag actgaccgga ttggtgattt
    13081 tgatctgcgc caacagcgcg caggcatcag catcaccgct gtcggcggct ttctgtgccc
    13141 aatattcggc tttgctcaaa tcgccggagc taaaccagct gtcggcaaga aaatattgtg
    13201 cgcgtcggtc cccggcctct gctgccttta aatactggct gccaggctcg gagtcgttcg
    13261 cgtgggcaaa gaatgtcaaa aacaacatca atgcgataat ttttttcatt ttgataatac
    13321 gattgggtag ggagtaacca gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa
    13381 ggaggctgta gaaaggactg tattacgcca gtgccgcttc gcgcaggcga gttttcaact
    13441 tgttgtactc gtcaatcacg tactgctcgg cggcgcgctg atcggcgatc ggctcgacgc
    13501 gcacggcgca gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca
    13561 gctcgttaca ggcaccaatc caccactggt aggtcatgta aatcgcccct ttgttcggac
    13621 gatcgctgac ctgcgcacgg gtgataattt tgcctttacg cgagtgcact caaaccaatg
    13681 cctcatcttc aatacccaga cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt
    13741 catcagccag cgccgccagt gccgcacagt taccggtcat cgaacggcaa gagtagtgac
    13801 caacttcacg caccgttgac agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg
    13861 ctacccagtc gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa
    13921 acagataaga agtcccctga tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc
    13981 ccagttcgcc cattttctcg taagtcgcac cgtagaaatc cgggcacaga tgacgcaact
    14041 catcccagat ctcctgggtg ttgttgtagt gcatcggata acccatacgg gtggcgattt
    14101 cactgatgat ttgccagtcc gttttcagat cccatttcgg ttcaactgct ttgaagaaac
    14161 gctggaagcc acggtcagcc gcagtaaaca cgccttcatg ctcgccccac gacgttgacg
    14221 gcaaaatgac atccgccgcc gacgcggttt tggtcataaa gatgtcctga acgatgacca
    14281 gttccagatc ttcaaaggct ttacgtactg ccgacagctc cgcatcagtt tgcagcggat
    14341 cttcgcccat aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc ggcaactcgc
    14401 tgatgcgata gccggtatga gcaggcaggc tttccacgcc ccaggctttg gcgaatttct
    14461 cgcggttagc cggatctttc acgtactggt atcccggata cgtatccggc agcgcgccca
    14521 tatcgcaggc accctgcacg ttgttctgac cacgaaccgg gtttacaccc gcatgcggct
    14581 taccgaggtt accggtcagc atcgcgaggc tggtcagaga acgcacggtt tccacgccct
    14641 ggtagaactg ggttacaccc atgccccaca ggatggcggc gcttttcgcc tgggcataca
    14701 tccgtgccgc ctgacgaatc tcactggcgc tgacaccggt gatatcttca accgactccg
    14761 gcgtgtagcc ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg gaagcgacga
    14821 acgctttgtc gtacagattt tcttcaataa tgacatggcc catcgcattc aacagcgcga
    14881 tgttcgagcc gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc
    14941 gcggatcgca gacgataatt ttcgccccgt tacgtttagc gttaattacg tgattcgcca
    15001 cgattgggtg ggaatccgcc gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa
    15061 tttcgttaat agcattgctc attgcgccat taccgaccga ttggtgcaga cctgcaaccg
    15121 atgggccgtg tcagacacga gcgcagcagt caacgttatt ggtaccaata acggcgcgcg
    15181 caaatttttg cattacatag ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct
    15241 ggatggcatc cggaccgtac ttctctttga tggcgctcag gcgctcggca acgtaattca
    15301 gtgcctcatc tcaggaaaca ggttcgagtt tgccgccacg ctggcgacgg atcatggggg
    15361 ttttcaggcg cggggtcagg atctgggtat cgttaatgaa atcccagcca taataaccct
    15421 tcagacacag ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg acgattttgc
    15481 cgttatcgac gaccaggttg attttgcaac ctgatgcgca ataggggcaa accgtgacga
    15541 cttttttcat cggtctcgct ccagttaatc aaatcacgca tacgcgctct cgactacagt
    15601 atgcatcttt tatgccacat tttatttggg gtcattccct gatattacgg gcactattta
    15661 ttcaaaactc tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa cgaaataccg
    15721 cgtgacagcc atcacgcggc agacatttta ttttttctcg acgacgggag ccgcctgata
    15781 cccgccgccc agcgatttca tcaactgaat gctttggatc acccggcggc tgtccagcat
    15841 cagtaatgac atctcttcgg caagtactgg caaccgggct tcagtggcct gtaagcggct
    15901 ggttaagccg cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac
    15961 gtgttcagcc tgcatttctc gctcgtcgtt gagcgtttgc agatgcgtgc cgttgacggc
    16021 aacgtcacgc accgcgttca gtactgactg gttgtaacgt tcaatcatca tgttgctggc
    16081 ggcgcgcgtg ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag
    16141 acccgggatg aagttgaact ggcgactggt ttttttgaat aaggtatcca ggtggatggc
    16201 atcaaggccg aaaaacgctt tgatatcaaa gctcggatag aacaacgccc gcgcggaatc
    16261 cacctgatct aatgacgcct gaacatacca gcgcatggct tgcagatccg ggcgtctggc
    16321 gagcaactca taagagagtg tcgccggaat gccggtctgg actcgtggta atgccacagg
    16381 tttgatctcc ggcatatcgc tggctcccgc gccaatcaat gcgcgcagag attctctcgt
    16441 ttcggtgatt tgccctttga cggcagcaat ttgtttatcg accgccagaa tctgtgcccg
    16501 cgcgccgtgg aaaggcactt gtgcttccag accgtgcgcc actttacttt ggtgcgcttt
    16561 caccgcgtaa tcaatcacat cgcgagtttg ttctaacaga tcgagcatct gatagctggc
    16621 ctgcatactg taataaagct gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt
    16681 ttctgccagc gcggcattat gcgcgccaat ggcggcggca accgctgagc gatgcacacc
    16741 ccacaaatca agatccagtc cggcaaacaa acctactgtg gcttccgtat agtacggccc
    16801 gtccatcccc agtgcgggcg catccatcgc ataagggctt aaaaagccgt tcgccgagac
    16861 acgttggcgg ttaagcatcc ctaacgctgc cacctgcaac tgtgaaccgg catctaacaa
    16921 atcggcctgc gattgcgctt tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc
    16981 acttagcgtc cgttggatca gggcatccaa ctgcgggtca ttgagttgtt tccaccactg
    17041 cgcctgcggc cagccggagc tggcaagatg aatatcatcg gccagtttga tttgttccgg
    17101 tttgagctgt tgatgaggcg cagaatcctt acgtaccagg gcacagccgg aaatcagcgt
    17161 cgtgctgccg agaatgctgc acaacagcag acgtgaaagt tgacgattga tcattgcgtg
    17221 gctccttgtg cctgttctgt ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg
    17281 gcgaaaagct ggcagacgtg ttgctcctgt tttaataagg tgggcaggat ggcctgcgag
    17341 gcgggtgtct cttcaagcgt tatttgagga gaacggctga gtgcggttgc cagaccggca
    17401 gcgtatttct ccagggcgtc ggcaaactga cctgctctgt ccggctgtag tgcgttatcc
    17461 agcgcctgcg ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg gccctgacga
    17521 ataaccgttt gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc
    17581 agcgccacgc gttggcacat ctcttcacag gcattaaacg ccgcatgcag accgatacga
    17641 atttgcagat aagtgcgcag agccgtgact tcctgctggc gtgggatccg cattacttta
    17701 cttaacatac ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc actttcaggc
    17761 cagacaaagg tgtaaatcat cgcggacacc acggtaccaa tgatgatacc cagggcgcga
    17821 tcgcgaattt ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt ggcgagcgcg
    17881 aaggtgacca ccatctgtgt gccgatataa gaagagcgtt cagagctggt agcaatccat
    17941 gcgcccaaca ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt gtccagccag
    18001 ggcatgacca gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa
    18061 cgcagcacca tcttctggta cgacgaaccg acatttggat tagcgacgat cacgcaggtc
    18121 agcatacagg tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta acagatcaaa
    18181 caggcgagca atgtttttac cgcgtagcgt atatagtctg gattggtaaa agcatcggcg
    18241 accattgatg gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat ctggcccagt
    18301 tgtaacaacg tctggcagat attctccagg ttacattccc gtgccgccac cgcttcactt
    18361 tcactgatcc gccagtcgct ttgccagcac tgaccttcag caacggcatg ctgcagcttg
    18421 ttgatttctg aggctaattt ttgccgaaat tcaataattg cctgagaatc agcaaaagag
    18481 gtgggatcgt agcgattcag cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc
    18541 caccatgcgc tttgagttcg ccagttggca tcgtccgcga gacaaaagac attcagtttt
    18601 tgcagcgcca gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag gctgtccgtc
    18661 aggtggctaa tggcatcatc aagccgatca ttaagcgctt gatgcatttg cgtaatggca
    18721 cgactgggaa accacagcac gccgattaac gtcatcagca aggttggata gaggccaaca
    18781 acgatacacc acagcgttaa gcgcacgact acttccggat agtcgagcat ggcggggaag
    18841 gtttgcccgt aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg
    18901 cgcatcaaaa acatgcagcc catcaggatc ggcccggcga tgatcaatcg gatcaacggt
    18961 tcgccgtatg accatttgta gatcaaaaac aggctgccga tctccagcac cgtggcaacc
    19021 acaaacaaga tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc
    19081 actgccagcg ataacgccac aaaagggatc tcaaaagtca tcgagatcag aatcaccagc
    19141 aggcagccta cccagagttg cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac
    19201 tcttcatgaa agaacgccag cagcctgacc accggtaatg gcagggagtt gagcgcgctc
    19261 attattgcgg ctcaagatta gcgactgccg aagcgccgat gcggaacatt tccgggtcag
    19321 gtttatcgac tatgattttg accggaaaac gctgggcaac gcggacccag ttaatagaac
    19381 gagacacttt tggcaggccg cccagcacca ggccgccgtc atccggtagc acgccgtagc
    19441 caatcgaatc cactttaccc tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg
    19501 cgggtgtacc tgagcgaata tttttcagat cggtttcgcg gaagttggcg atcacgtacc
    19561 agtgacgagt gtcgatgagg gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg
    19621 aggttttgag ggaaatgatc cggccatcaa atggcgcgcg aacggtcgtc atttccagat
    19681 gtagtttggt cagggcaata tccgcttcga ccgccgcacg ttgcgccacc agcgcatcga
    19741 cgccgctggc ggctgactgc gcctgtaaca atacggcatt aagatccgct tctgcggcgc
    19801 gctgcgccgt tcttgcacgg tcaacgtctt ctgctgagac aaaaccctct ttcagtaatg
    19861 gctcggtgcg gcgtaatgta tctgtggcct gtttcgcggc ggcacgggct ttttctaccg
    19921 tggcattaac cgagtcggca ccaaactgtt gggcgtcaac gctacgctgg gtgagcataa
    19981 tttgcttatc cagcgccgcg agggaggctt cggctttcgc caggttggct tcgtacgggc
    20041 gcgggtcgat gcggaacagc aaatcgccct gtttcaccgc ctggttgtcg gtgaccgcca
    20101 gttctacaat gcgaccgctg acttccggca ccacatcaat ggtatctgct gacgcgtaag
    20161 cgtcattagt tgatggcgca ctgtctacgc gccagataac gaaaacaagg gcaaccagcg
    20221 ccaacgcaac cactaacaga gcagggaatt tattgcgagg agcttttttc ggcgtacttt
    20281 ccatgagcgc gtctcaaata gattagaaaa atgccagcca gaacagcatg gcgtagagag
    20341 caaaaagggc ggtataaata attccgacaa atgccagatt gatatttgcc cgctgaataa
    20401 ctcgcctcgt gatgagcgtc aaaataaggc tggcaatggc gcagaaaaac cagctgggat
    20461 aataagcgcc gatcaccggg attgccggag ataatgagca tcccgaaacc atgaagacgg
    20521 gaattatcca ggctgttttt ttgaggtgca tcgccatgcg agagagaact gttggcatat
    20581 taaatacatc ctgtctgctg cgaacaatat ctgtagtgaa tatcaatatc cattgaaggc
    20641 gtcagtatgg ctattttctc gatgaataaa aatagcttta tttagccacc gcattcataa
    20701 cttatatatt ttatttatta attggctgta tatattttta aaataaatgc ttttgggggc
    20761 ttcagcgccc ccaaaagcga gtgacattaa tttggggtta cgatattcac ccacaaatcg
    20821 aaattatcca gacaggcgat aatttcttcc agtttcgcgc cattgccaat aattttggct
    20881 ctcttcgctt tcaccagatc cgccattttg gcctgtccgg tcagcacggc gtgcagatct
    20941 tcacggctga tgtagaaaga ggcgtcgact tgcggttgca gtgttttacg g
//
LOCUS       DADLSA010000060        20851 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.060, whole genome shotgun sequence.
ACCESSION   DADLSA010000060 DADLSA010000000
VERSION     DADLSA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.060"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..882
                     /locus_tag="JN691_002226"
     CDS             <1..882
                     /locus_tag="JN691_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7243668.1"
                     /translation="RNYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFST
                     FLRAQISLAWVIGPPLAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVV
                     TQPVEILPSTHRKRDTRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGI
                     AAGLEIPMMLIAGYYMKRIDKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIF
                     LGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLA
                     IGMLGIAMICLLFIKDI"
     gene            993..1916
                     /locus_tag="JN691_002227"
     CDS             993..1916
                     /locus_tag="JN691_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7243669.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1920..2738)
                     /gene="nlpA"
                     /locus_tag="JN691_002228"
     CDS             complement(1920..2738)
                     /gene="nlpA"
                     /locus_tag="JN691_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HAY7243670.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAQEDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2960..3252
                     /locus_tag="JN691_002229"
                     /pseudo
     CDS             2960..3252
                     /locus_tag="JN691_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3293..4483)
                     /gene="nepI"
                     /locus_tag="JN691_002230"
     CDS             complement(3293..4483)
                     /gene="nepI"
                     /locus_tag="JN691_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HAY7243671.1"
                     /translation="MSEFIAENCGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNIFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIGSFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4694..5146)
                     /locus_tag="JN691_002231"
     CDS             complement(4694..5146)
                     /locus_tag="JN691_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAY7243672.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(5199..6533)
                     /locus_tag="JN691_002232"
     CDS             complement(5199..6533)
                     /locus_tag="JN691_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7243673.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6708..8460
                     /gene="adeD"
                     /locus_tag="JN691_002233"
                     /pseudo
     CDS             6708..8460
                     /gene="adeD"
                     /locus_tag="JN691_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
     gene            complement(8506..9897)
                     /gene="uhpT"
                     /locus_tag="JN691_002234"
     CDS             complement(8506..9897)
                     /gene="uhpT"
                     /locus_tag="JN691_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAY7243674.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(10035..11354)
                     /gene="uhpC"
                     /locus_tag="JN691_002235"
     CDS             complement(10035..11354)
                     /gene="uhpC"
                     /locus_tag="JN691_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAY7243675.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(11364..12866)
                     /gene="uhpB"
                     /locus_tag="JN691_002236"
     CDS             complement(11364..12866)
                     /gene="uhpB"
                     /locus_tag="JN691_002236"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAY7243676.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12866..13456)
                     /gene="uhpA"
                     /locus_tag="JN691_002237"
     CDS             complement(12866..13456)
                     /gene="uhpA"
                     /locus_tag="JN691_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAY7243677.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13531..13821)
                     /locus_tag="JN691_002238"
     CDS             complement(13531..13821)
                     /locus_tag="JN691_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase isozyme 1 small subunit"
                     /protein_id="HAY7243678.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFSRRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13825..15513)
                     /gene="ilvB"
                     /locus_tag="JN691_002239"
     CDS             complement(13825..15513)
                     /gene="ilvB"
                     /locus_tag="JN691_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAY7243679.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(15619..15717)
                     /gene="ivbL"
                     /locus_tag="JN691_002240"
     CDS             complement(15619..15717)
                     /gene="ivbL"
                     /locus_tag="JN691_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAY7243680.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            16282..16371
                     /gene="tisB"
                     /locus_tag="JN691_002241"
     CDS             16282..16371
                     /gene="tisB"
                     /locus_tag="JN691_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HAY7243681.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            16651..17835
                     /gene="emrD"
                     /locus_tag="JN691_002242"
     CDS             16651..17835
                     /gene="emrD"
                     /locus_tag="JN691_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAY7243682.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17843..18340)
                     /locus_tag="JN691_002243"
     CDS             complement(17843..18340)
                     /locus_tag="JN691_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY7243683.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(18337..18699)
                     /locus_tag="JN691_002244"
     CDS             complement(18337..18699)
                     /locus_tag="JN691_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAY7243684.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18689..19036)
                     /locus_tag="JN691_002245"
     CDS             complement(18689..19036)
                     /locus_tag="JN691_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HAY7243685.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            19145..19558
                     /locus_tag="JN691_002246"
     CDS             19145..19558
                     /locus_tag="JN691_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243686.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(19605..>20851)
                     /locus_tag="JN691_002247"
     CDS             complement(19605..>20851)
                     /locus_tag="JN691_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY7243687.1"
                     /translation="MGRYFKDAGYHTCYIGKWHLDGHDYFGTGECPPEWDADYWFDGA
                     NYLSELTEKEISLWRNGLNSVEDLQANHIDETFTWAHRISNRAVDFLQQPVRAEEPFL
                     MVVSYDEPHHPFTCPVEYLEKYADFYYELGEKSQDDLANKPEHHRLWAQAMPSPVGDD
                     GLYHHPLYFACNDFVDDQIGRVINALTAEQRENTWVIYTSDHGEMMGAHKLISKGAAM
                     YDDITRIPLIIRSPQGERRQVDTPVSHIDLLPTMMALADIEKPEILPGENILAVKEPR
                     GVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLVLNLFTSDELYDRRNDPNEMHNLIDD
                     IRFADVRSKMHDALLDYMDKIRDPFRSYQWSLRPWRKDARPRWMGAFRPRPQDGYSPV
                     VRDYDTGLPTQGVKVEEKKQKF"
BASE COUNT         5194 a         5610 c         5202 g         4845 t
ORIGIN      
        1 cgcaactact tcattctcct ctctacgggc gtacttctga gtagttttgc ttctaccgca
       61 aacccgcaaa tgttcgccct cgcccgtgaa cacgccgaca gaacaggccg tgagacggtc
      121 atgttcagta catttttacg tgctcaaatc tcgcttgcct gggttatcgg gccaccgctc
      181 gcttatgaac tggcaatggg gtttagtttt aaagtgatgt atctcaccgc tgccatcgca
      241 tttgttgttt gcgggctgat agtctggttg tttttgccat caatacaaag aaatattcct
      301 gtcgttaccc aacccgtaga aattttaccc tccacccata ggaagcggga tacgcggcta
      361 ctttttgtgg tctgttcaat gatgtgggcg gcgaataatc tctacatgat aaatatgccg
      421 ctatttatta ttgatgaact gcatctaacc gataaactgg ctggagaaat gattggtatc
      481 gctgccggtc tggaaattcc gatgatgtta atcgcaggct attacatgaa acgtattgac
      541 aagcgactat taatgctcat tgctatcgtg agtggtatgt gtttttacgc cagcgtactc
      601 atggcgacga ctccggcggt tgagctggaa ttgcaaattc taaatgccat cttccttggt
      661 attctctgtg gtatcggcat gctttatttt caggacttga tgcctgaaaa aataggctct
      721 gcgacaacgt tatatgcaaa tacttcacgc gtcggctgga ttatcgccgg ctctgttgac
      781 ggaattatgg ttgaaatctg gagctaccat gcgttgttct ggctggcgat agggatgttg
      841 ggtattgcga tgatttgcct gctgtttatt aaagatattt agctcaccag tctgggctaa
      901 gaaaaatcgg cgtggcgcaa acagagccat cctcgccatt tttgctatca tgcctgcata
      961 cataaacgac aaaacagtat gcagagggaa aaatgggttc caccagaaag gggatgctga
     1021 acgttctgat tgccgccgtg ttgtggggaa gttcaggggt ctgtgcgcaa tacatcatgg
     1081 agcaaagcca gatgtcgtcg cagtttttga ctatgacgcg tttgatattc gccggtttga
     1141 ttctgctgac gctgtcattt gttcatggcg ataaaatctt ttctattatt aacaatcaca
     1201 aagatgccat tagtctgctg attttttccg tggttggcgc gctaaccgta cagctcactt
     1261 ttttgctaac catcgaaaaa tcgaacgcag ccacggcaac ggtgctgcaa ttcctctcac
     1321 cgacgattat cgtcgcctgg ttctcactgg tacgtaaatc gcgcccgggc attctggttt
     1381 tctgcgctat tttgacatcg ctggtcggga cttttttatt ggtgacacac ggtaatccga
     1441 cgtcattatc gatctctcct gccgcgttat tctggggcat tgcctcggca tttgctgctg
     1501 cattctatac cacctatccc tcaacgctga ttgcccgcta tggcacgttg cctgtcgtcg
     1561 gctggagtat gctgatcggc ggtctgattc tgttgccttt ttatgccaga caagggacaa
     1621 actttgtcgt taacggcagt ttgattttgg cgttttttta tttgatcgtc attggtacgt
     1681 ccctgacatt tagtctgtac ctgaaaggag cacaattaat tggcggtcca aaagccagca
     1741 ttttgagctg tgcagaacca ttaagtagcg cgctactctc tttgctgttg ctggggatca
     1801 cgttcacatt accggactgg ctgggaacgc tgctgattct gtcatcggta attttgattt
     1861 caatggattc ccgtcgccgc gccagaaaaa taaatcgtcc ggcgcgggat gagtgagaat
     1921 taccagccag gcaccgcgcc accgttaaag atggtttctg ccgctttagc gacttcggat
     1981 gattgataag attgcagaaa ttccttcacg ttttctgcat tcttattatc ttcctgtgcc
     2041 accaaaatat tcacatacgg cgaattttta tcttcaataa atacgctgtc gtgcaccgga
     2101 gaaagcccgg tctgctgaat gtaagtggtg ctgataatcg ccacatcaac tttgggatca
     2161 tccagtacgc gcgggagctg cgccccctcg agttccataa tctgcaaatg acgaggatta
     2221 tcggttatat ccagcgcggt aggtaataac ccttttccct ctttcagagt aattagcttc
     2281 tctttttgca acagtaaaag cgcacgacca aggttggtcg ggtcgttggg gatcgccact
     2341 gtcgcgcctt cttttatttg cgccacggtt ttgatttttt tggaataacc cgccatgggg
     2401 aagacaaacg tatttcccac cgctaccagt ttataaccat gcgcctgatt atcttgttca
     2461 agaaagggtc gatgctggaa gacgttcgca tcaagttcgc catgatttgt cgcatcgttc
     2521 ggtagcaacg aaccgctaaa tcctaccagt tgaacatcga ggccatattt ctcttttgcc
     2581 acctttttag cgacttccgc gacatcttgt tctgcgccat ttataacgcc aactttaatg
     2641 tgttttgcat cgctgctact ctggtcgcaa cctgccagca gaattccggc cagcaataat
     2701 gcggcccctg tccgtagatg atgtgttgtc agtttcattt ttttatcctt ttattaatga
     2761 ttacctgact atagcggtaa ggtcgctgcg gtttaaaaaa cgaaacgcta tcgataagaa
     2821 taaaaaggaa taaaagtgga atataaagtc tggcacttct tactgaccac gcaagcgcgt
     2881 ttcgtacagc atgatgagag cgatgaaagc aaactacact tatgcttcat tcgatacaca
     2941 tttgtaaaag gttgaatcca tgaaaccaac gacactactg cttattttca cttttttcgc
     3001 tatgccaggc atcgtatacg ccgagtcgcc ttttagctca ctgcagtccg caaaagagaa
     3061 aaccacggta ttacaagatt tacgtaaaat ttgcacgcca caggcgtcat tatcagatga
     3121 agcgtgggaa aagttaatgt tgtcagatga aaacaataaa caacatatac gcgaggccat
     3181 cgtggcgatg gaacgtaata atcagagcaa ttattgggaa gcgttgggta ggtagaatgc
     3241 ccggatatgt aagaacagca acaactactg accaacgctt ctttacctga tatcaggatt
     3301 tcttcatttt cacctttgca gtaaccaaca atgcagtcag caacatcaat gtgccggaca
     3361 acatcaacgg cgaagtcaga ccaatattat ccagcgcata accgccgatt gccgcgccac
     3421 aggtattagc aagctgaata accgccacct gaatagaccc ggctttttct gcctgatcgg
     3481 ccagcgagcg ggtgatccac gttgaccagc cgacgggaac caatgcaaaa gttagccccc
     3541 agataatcgc cacgccggta gcaacgattt tatcgcttcc ccacaacgtc agtaccaacg
     3601 cactcacagc cagtattaac ggcgcgcctg ctaaggccag ttttaccgaa cgtttaagaa
     3661 tgaacgacga aagcgacgta ccaataaagc tgccgatacc aaaactcaac agcaccagcg
     3721 ttaagccatc cacgccgaat cccgctaggt tcatatacac cgggcgaata tacgtgaaga
     3781 aagcaaactg cccggcgaaa gacatgaaga tggcgatcat ccctgccatc acacccggac
     3841 gttgtaataa gcggaaaata ttttgtttct gatgcgaggg ttcgcctggc agtgaaggca
     3901 atgatttgat aatccagaaa atacacagca cgcccatcac cgccgccgca ttaaagacat
     3961 tgcgccaacc gataagctcg cctaaaaaac tgcccaacgg cgcggcaatc accagcgcaa
     4021 tagaaaccgc gccgaagatc accgacagcg ccttcggcac cgtacgcggc ggtaccagac
     4081 gcatggtcag cgacgccgac atcgcccaga acccgcccag cgccagcccc agacaggcac
     4141 gaccgattaa aagcaaactg aatgagttag caaaggaaac cagcaagcag gagagcgtca
     4201 gcaaaacggc aaacaaaata acaacgtagc ggcggtcagt ggcctgaatt gtctgggtaa
     4261 taaacaaact ggcaaacatt gccacaaagg cggtcacggt caccgattgc ccggcaaccc
     4321 cttccgaaat gcctaaatcc tgggccattg gcgtcaacaa actgacgggc aaaaactcaa
     4381 cgataatcag acaggcgaca caaaacgcca ccgagaaaac ggctgaccag ttcggtcggg
     4441 tgatggcatc cgcgccgcag ttttcggcaa taaattcact catggtgtta cccgtggcat
     4501 gtttcagggg aaaagccgac cactttaaca ttgaaatgtg tgacgcattt aacgtttttg
     4561 caacttttac ggtgttgctt gagctggagt taagatcgaa acggaacagg gcaaagatta
     4621 ccggatgcgg cgtgaacgtc ttatccaccc gacaggtcag gcgcggtctg taggctgata
     4681 agacgaaacg gtttcaatgc aaccagtgaa cgccctcggc aggctgaaag aacgcgttat
     4741 agcgcatctg aaaagcatta atatcctgca tttcaggttc ggtttcacgc agaaaaccca
     4801 gcgccagcct gacctgagca tcggtaatcg gcgcggaaag gcgggctttt tgtaaaatcc
     4861 ggtgccattg cagcaggttc tgttcgctat catcgacaat gacaatttgc cagatgagca
     4921 acacctgcgc ggcgttttgc aggtgcgact catccgggcg atgcaaataa cgtaaaaatt
     4981 cgtcaccggc tgactttccc atttgatcga ccatcgactc cacgggtacg acatcgatat
     5041 tcagccgatc gctgagacgg cgaatcgctt ttctggcccc ggatgtcagc acccgaaaac
     5101 caaccacaaa cactaccgcc agcgtggcga gcattatcca gatcatgagt tctcctgcgt
     5161 aatcagaact catgatatct ggaaaccctc gccagttact aatccaccag aataatcttc
     5221 aatgcgaaca gagctgcgac caccaccaca cacaggttca gatcgcgcca gcgcccggtg
     5281 cacactttca tgatgcagta cgacataaag ccgagtgcaa tcccttcggt gatcgagaaa
     5341 gtaaagggca tcatcaccgt ggtaataaac gcaggcaccg attcggtaaa atcatcccag
     5401 ttaacgcgcg ccaggctcga agtcatcagc acgccaacaa agattaacgc tccagcggtt
     5461 gcgtaaggag gaaccatcgc caccagcggt gagaagaaca taactaacag gaacataacg
     5521 ccgaccacga cagccgttag ccccgtgcga ccaccgactg ccacaccaga agtactttca
     5581 atataggcag taacagacga ggtaccgata aatgcgcctg ccaccgaact gacgctatca
     5641 acatacagcg ccttattcat attggggaat ttaccgttgc tatctattaa gcccgcttta
     5701 tcagttacac caattaatgt tcctgatgaa tcaaataggt tgatcagcat aaaggagaaa
     5761 atgataccgg cgagttcaag tgttaacgcg ccgctcaaat ctacttcacc aatgacgccg
     5821 ctaatatcag gcggaatgga atagacgccg ctaaaatgaa catcaccgaa aaataatcca
     5881 cagcaagacg tcaccacaat agaaaccagc accgcggcat gaaaatgacg tgatgacaac
     5941 acggttataa taaaaaaccc taaaatacct aacaacacgc cgtgagaact taaatcgcca
     6001 atcatcacca gcgtgtcttt attggcgaca ataacaccag tgttttttaa tcccattaag
     6061 gcaataaata atccgattcc actggtgata ccaatacgta aacttaatgg aatgttggag
     6121 atcatccagt accggatacg aaacagcgtg agcaaaaata gcccaattgc gccccagaat
     6181 atcgcgccca tcccggtctg ccaggagatc cccatcgccc ccacgaccac gaacgcaaag
     6241 aaggcgttca gccccattgc cggagccagc gccacgggta agttagcaaa tatccccatc
     6301 gcaatactgc cgataccggc aatcaaacag gtggtaacaa acaccacttt cgggtccatt
     6361 tgtgccgcgc cgaggatttg cgggttcaca aaaacaatgt acaccatggt taaaaaagtg
     6421 gtcatccccg caatcaattc tgtgcggacg gtggtcccat gctgaggtag tttaaataat
     6481 cgcgaaagcg tccctgattc attactcacg taatcggtat tgtcattatt catttttttg
     6541 tccattggag gagatttaat cccttgcttt tgtaaaacaa atgacaacaa cggttcagtg
     6601 ataattatca catttcaatt gcacattaat agatattctt taataatctc gcgacgtttc
     6661 tttatgataa ataataatca aattgataaa atcaaaatga gaaaaatatg aataattcca
     6721 ttaaccataa atttcatcac attagccggg ctgaatacca ggaattgtta gccgtttccc
     6781 gtggcgacgc tgttgccgat tatattattg ataatgtctc tattctcgac ctgatcaatg
     6841 ccggagaaat ttccggccca attgtgatta aaggacgtta cattgccggt gttggcgcag
     6901 aatacgctga tgctccggct ttgcagcgga ttgatgctcg cggcgcaacg gcggtgccag
     6961 ggtttattga tgctcacctg catattgaat ccagcatgat gacgccggtc acttttgaaa
     7021 ccgctaccct gccgcgcggc ctgacgaccg ttatttgcga ccctcatgaa atcgtcaacg
     7081 tgatgggaga agccggattc gcctggtttg cccgctgtgc cgaacaggca aggcaaaacc
     7141 agtacttaca ggtcagctct tgcgtgcccg ctctggaagg ctgcgatgtt aacggtgcca
     7201 gttttaccct tgaacagatg ctcgcctggc gggaccatcc gcaggttacc ggccttgcag
     7261 aaatgatgga ctaccctggc gtaattaacg ggcagaatgc gctgctcgat aaactcgatg
     7321 catttcgcta cctgacgctg gacggtcact gtccgggttt gggtggcaaa gaacttaacg
     7381 cctatattgc tgcgggtatt gaaaactgcc acgaaagtta tcagctggaa gaaggacgcc
     7441 ggaaattaca actcggcatg tcgttgatga tccgcgaagg gtccgctgcc cgcaatctca
     7501 acgcactggc accgttgatc aacgaattta acagcccgca atgcatgctc tgtaccgatg
     7561 accgtaaccc gtgggagatc gcccatgaag ggcatatcga tgccttaatt cgccgcctga
     7621 tcgaacaaca caatgtgccg ctgcatgtgg catatcgcgt cgccagctgg tcgacggcgc
     7681 gccactttgg tctgaatcac ctcggcttac tggcacccgg caagcaggcc gatatcgtcc
     7741 tgttgagcga tgcgcgtaag gtcacggtgc agcaggtact ggtgaaaggc gaaccgattg
     7801 atgcgcaaac cttacaggcg gaagagtcgg cgagactggc gctgtctgcc ccgccatatg
     7861 gcaataccat tgcccgccag cgactttgcc ctgcaattca cgcccggaaa acgctatcgg
     7921 gtcattgacg tcatccataa cgaattgatt acgcactccc gctccagcgt ctacagcgaa
     7981 aatggttttg atcgcgatga tgtgtgcttt attgccgtac ttgagcgtta cgggcaacgg
     8041 ctggctccgg cctgtggttt gctcggcggc tttggcctga atgaaggcgc gctggctgcg
     8101 acagtcagcc atgacagcca taatattgtg gtgatcggtc gcagtgccga agagatggcg
     8161 ctggcggtca atcaggtgat tcaggatggc ggcgggctgt gcgtggtacg taacggccag
     8221 gtacaaagtc atctgccgtt acccattgcc gggctgatga gcaccgacac ggcgcagtcg
     8281 ctggcggaac aaattgacgc cttgaaagcc gccgcccgtg aatgcggtcc gttacccgat
     8341 gagccgttta ttcagatggc gtttctttct ctgccagtga tccccgcgct aaaactaacc
     8401 agtcaggggc tatttgatgg cgagaagttt gccttcacta cgctggaagt cacggaataa
     8461 taaaaaaagc ccggcgtcat gccgggcaaa agtcaccagt tacgtttatg ccactgtcaa
     8521 ctgctgaatt tttttctcgc ggcggatttt gcgttcttcc attaccgcca ctatcgccat
     8581 caggcagata caaccaatcg cggcgatatc cagcgcggcg aaggtgcctg cccagccggt
     8641 aaggccgaat accggcgtcc catcggcaat cattcccaga cctaacttgg caaagctgtc
     8701 accaatcagg taagcaaagg tgcctttaat accatcggca gcgccaatcg cttttttagg
     8761 tacaaagcca acagcagcca caccaatcaa caattgcggg ccaaagacca ggaaacccaa
     8821 cgcaaagaga gaagccagat agatatattc gttactggca tgttgataca caccgagcgt
     8881 ggcgataatc agcgccagcg cgatgcaggc caccaggcca cggcgaccgt tcgccaggtc
     8941 agagagccag ccccacagca gcgtaccgac cagcgcacca gcttcaaaca gcgtaaagcc
     9001 ctgaatcgcc accgctttag agagtttcag ttcctggaac gcgtatacgg ttgaccactg
     9061 gtcgatacca atacgtacca catagaggaa aatgttggcg aagcacagca gccagatcac
     9121 tttgtttttc agcacatact caacaaagat ctgccacttg gtcatatcgg tagattctgt
     9181 ctctttgtcc tcttcgctga tctcctcgcc gaacagttct tcagctttgc cgaggccata
     9241 agattccggg gagtcgctgc cgtaacgcag gccgataaaa ccgacaatca gcgcgataat
     9301 cgacgggaag ataaacatgc cgatgacatg gccatcgaac aggtaatttg ccccgaacag
     9361 cgccacacct gctgcgcctg caccgccaag attgtgagaa atattccaga aaccgaggaa
     9421 tgtcccgcgt ttacgacgcg gcgtccattt ggtgatggtg gagtaactgc acgaaccgcc
     9481 ggtactctgg aaaaagccgc ttaaggcgta gaaggcaatc atcaggaaca ggctaaccga
     9541 gccgctgccc atactggcac tgaagcccag catacaaatg gcagagagga taagcatgaa
     9601 cggcaggaat tgtttggtgt ttttgccgtc ggcgtagtat gaaaccaacg ttttacccac
     9661 gccataagtg atggagaaac ccaggccgat catccccagc tgcgtcatgc tcaacccgta
     9721 ggtcgaaatc atatcgttct gcgcgatgtt aaagttcttg cgaatcaggt acatcgtcag
     9781 gtagccgata aagaccacca ggtaggattg catgaacggt ttgaaccaca ttttgcgccg
     9841 cacttcgagc ggaaggtcca gggtcggctt gcgaacctgg tttaggaaag ccagcatggg
     9901 ttactcctga aatgaatacc tgcgtgaggc atgcattgta aaaatcagca gcaaatcagg
     9961 cctgagatag cgtccaagaa aaacctggaa aatttcttag ttttgccgga tatgaagtga
    10021 aaaggtgaga tgcatcacgc ttcacgcggt gtctgggcgt tcaaaaaggg cagtaacagc
    10081 agtgcggaaa tcccggcggc gatagagata accacaaaaa atccgctcca gtgccaggta
    10141 tcgagcactt tcgccagcgg ccaaccagca agcgacgccc ccagataagc aaacaagccg
    10201 acaaaccccg tcgccgcccc tgccgcctct ttgtgggaac actctgccgc cgccatacca
    10261 attaacatct gcgggccaaa gacaaaaaaa ccaatggtga agaagcaggt tgcctgcatc
    10321 acgtagctgg caaatggcat cagccacagc gagccgactg aaagcaaaat tccggcggcg
    10381 aaaatcaaat tcatcggccc acggttgccg ttaaacaatt tgtccgagcc ccaaccggct
    10441 accagcgcac cgataaatcc gcccagttca aacatcgtca ctgccgtatt cgccgtgacc
    10501 agatcgacgc ccagcgtctc ggacatatac aaattgcccc agtcgttgat cgccgcccgg
    10561 accacataga ccagcacata gcaaaacgaa agcagccaga tatacggatt cagcaacaca
    10621 tatttggtga ggatctcttt acgcgtcaac cctgcccctt cttgttgttg agcaatttcc
    10681 agcgcgtcgt gccgccattc accgaccgcc ggtaaaccta acgcctgcgg gcgatcgcgt
    10741 agccgccagc agagaaaaat ccccacgact atcgccatac aaccagcaat catcatcccg
    10801 gcacgccagc cgtaatgcag cgcgctcgct gccatcacaa tgggaatgag tgcgccgccg
    10861 acgttatgcg ccgtgttcca taatgcccac caaccgccgc gctcggtacg tgaataccag
    10921 gccgttaaca gacgcgcaca caccggtgaa ccccagccct ggaaaaaggc gttcagcacc
    10981 cagagtacgg caaacgccca tagcgacgtc gagaagccaa acagaatgtt catgatgccc
    11041 gtggcaataa gccctatccc cataaaataa cgggcatttg agcgatcgct gacaatgccg
    11101 gagacaaact tcgacacgcc gtaagtaatg taaaacaggg tcgctaacag gccgatatcg
    11161 ctacggctga gcacgccgtt agcaaggatt tctggtaccg cggcgttaaa acttttccgc
    11221 gtgaagtaaa acagcgcgta acccagccag atggtcagca gaatatgccg acgccagtag
    11281 cgataacggg catcaatttc atgtttatca gtcattaatg gcgcatcggc aggcgctttc
    11341 agaaacggca acatcatcga accttagaca taacgttgag gtagagaaac gctgacacgc
    11401 gtgccgtgca gacaggaaat gtgtaatgtg ccgcccagcg ccgttacgcg ctcgcgcatt
    11461 ccggtgaggc caaaaccttg ttgcccggaa cccggcggca aaccgctgcc atcgtcttca
    11521 atcaccagca tcaaccgttc atcctgctgc cagccttgca gcgtaaccgc gctggcatcg
    11581 gcatgtttca caatgttgtt cagcccttcc tggcagacac gaaacagcgt cacgcgctgg
    11641 ttttcgctta atgctgattc atcgatacgc cattcgagat ggctgacaat gccgcgccct
    11701 tccagctcca tttcccgcat cagtgagcgg atggcctgct ccagggtgag atcatccagc
    11761 tggcgcggac gtaaccgtcc taacaaacgg cgcaccgcgt cgtaaacgcc cagcgacagt
    11821 tgttcgatga gctgcccgct ctgcttcaca ctggcgttat ctgccgccag ccgctgaaca
    11881 atgcccgcct gagtacgaat agcagtgatg gtctgaccga tatcgtcgtg cagctcacgc
    11941 gccacgtcgc ggcgcacgct ctcttcggtt tctaacaaac gttcagccag atgctgattg
    12001 cgcgccagtt ccttttgtag cgactggtta agttcacgca accgctggat gcctgcgccc
    12061 agtaacaacc ctgtcagact ttgcaccagc aacgagagta ataaatccac cggatgatcg
    12121 cgccaggttt gactggcgat cagcgcgatg gcgttcatca acgtcgcaat cagcgctcct
    12181 tgccagccat agtgccaggc cagcgcgata atcggcagcg ccagacagaa tggcgtaaag
    12241 cgcgacagtt cgtccggcaa tcccaactgg agccagagac tgataacaaa cagcagcaag
    12301 taccagacca gatgccgccc gcgccagttg atgggctgag aaaccagtga cgggccgagc
    12361 ggcagccagg tgttattggc gagatagtgc cagaacacca gacatatcgg ggccagcgtc
    12421 agaccgccag ttaaagtcag caacagcgca ttccacgact ctttgccgtg ccaaagccag
    12481 ggcagcgact gcaacaacgc cgccgccgtt aacgccgcac cctgtaacag caaggtgcgc
    12541 cagtcacgct gatggcgata gcgcgagatc agcgctacgg gcagtaacgt cagtaaacta
    12601 ccgatcatca ataacggaaa atgggttaaa ccgaccgcct gcgttagcca gtaaatcagc
    12661 agccactccg cacccagcaa tacgggccag tatccgcgcg ggcattgcag cattagcccc
    12721 agacgcagac caaacggaaa taacagcacc gccatatcag ggcgctcaac cagatgcagg
    12781 ctgatactcc ataggcaaaa ccatgcggca gagaagataa aaaagcaggc aataacggta
    12841 attaagcggg agaacaacgt cttcatcacc agccatcaaa catgcggcgc gccagctcta
    12901 cgtcgttact gacgcccagt ttttccatca gattggcgcg atggacgtgt accgttttcg
    12961 gtgacaagcc cagttcggcg gcaatctctt tcaccgccat tccttgcgcc agtttttccg
    13021 ccacctggcg ttcacgtttg gtcagcgggt cctgacgacc ggatgccagt ttaatggcaa
    13081 tatccggcgt cagataacag ccgcccgtgg caaccgtatg caccgcagca atgagttcat
    13141 ccgggctaca gcgtttggaa agaaagccgc gtgcccccgc gttaagcgcc tgctcaacca
    13201 gcgcaggact gtcgtgaacg gagagcataa tcgtcgccat acctttcggc agctggctta
    13261 gcagctccag accggagata tcgggcatgg agatatcgca aatacacacc tgcacaccgc
    13321 gccccggcag ccccgccagc gcctcgcgcc ccgaaccaaa ctcggcaact acctgcaaat
    13381 caggttccag ccccagcagc tgcgcaaagc cggagcggac gatgaggtga tcgtctataa
    13441 gggcaacggt gatcatggtc ttgtcctggc gggtaaaaaa aaacgcgctt accttaacga
    13501 taagcgcgat gttgttcaag ccttgagcgg ttactgaaaa aacaccgcga tcttgttaaa
    13561 catcgtcgga tcggactgat tacgctgcac tttcacgaca tcttccagct tatcgatttg
    13621 gcttatcatc tgctccagac gctggtcgtc attgaccagt agccagatat ggcttttgtc
    13681 gctgtcctga atcggcagac aaagaatgcc ttcaacgtta aaagcgcggc gggaaaaaag
    13741 gccacaaacg tgggtcatta cgcccggatg gttgcgaacg gtgagctcca gaattacgtt
    13801 gttatgagtt gtgttttgca tggcttattc ccccaccatt tcagtattcg ccgcacctgg
    13861 cggcaccatc gggtaaactt tttcttcggc atcaatgcgc acatggatca gcgccgggcc
    13921 agggcgattg atgatttcct gcaatgcagc ctgcggatcg gcttcgttat tcaaatcaca
    13981 ggtttcgagg ccgaatccgg cggcaatctg cataaagttg attttgcccg gataggtggc
    14041 ggcaaaaacg ccttgctcgt agaacagact ctgttgctga tgcaccagcc ccagcgcttc
    14101 gttgttcatc agaatgattt tgacatccag ctgattttca ctggcggtcg ccatctcctg
    14161 aatattcatc atcaggctgc cgtcgccgga gaaacacaac actttgcgat ccgggttcgc
    14221 cagcgccgcg ccaatcgccg caggcaggcc aaaacccatc gtgcccagcc caccggaggt
    14281 cagccactgg cgtgggcgat tgagcggata agcttgcgcg gtccacatct gatgctgacc
    14341 cacatcggtg gtgataatcg cattgtcatc gacacaggcg gcaacggcgt tgatcaggcc
    14401 gtaatggctt aatggatcgc acgctttcgg gattggacac gggaattcgc gctgcaaatc
    14461 cgctaccaac tggtgccact ctgcacgcgg ttgcgcttcc acctgcggga tcaattgcgc
    14521 cagcacgtca tcaacatccg cctgaatcgc cacatgcggc tgcttgattt tacccagctc
    14581 tgcgcgatcg atatcgacat gaataatttt ggcattcgga cagaactgct cggttttgcc
    14641 aatcgcccgg tcatcaaaac gcgcaccgag cacaatcaac aaatccgcct cctgcaaaat
    14701 atagttggta ctgcgcacgc cgtgcatccc cagcataccc agcgacaacg gatgcgcttt
    14761 tggcagcatg cccagcgcca ttaaagtcat ggtggtaggc agttgcgctt tctccgccag
    14821 ttcacgcacc cgcgcaggcg cattgatcac accaccaccc agataaagca ccgggcgttt
    14881 ggcagcgtta atcattgtag ctgcgtcacg aatgctttct tcgctaaagg cgggtgcagc
    14941 ggctttttct gccaccgcgg gctgagcttc aatctcaaaa accgccgttt gtacatcctt
    15001 aggaatgtct atccacaccg ggccagggcg gcctgattgc gcaatgcgga aggcatcgct
    15061 catgacctgt gggagctctt cgatatgtct gaccagatag ttgtgtttgg tgatggggat
    15121 agagatgccg taggtgtcca cttcctggaa ggcgtcggtg ccgatcatcg aggcgggaac
    15181 ctgaccagtg atgcaaatca gcgggatgga gtccagccgc gcatcagcaa tggcggtcac
    15241 caggttagtc gcacccggcc cgctacaggc catacagacc gccggtttac catcggtgcg
    15301 cgccattccc tgagcgataa accccgcgcc ctgttcatgg cgagccagaa tatggcggat
    15361 ttgcgtgctt tggcttaagg cgtcgtaaac aggcaggata gaaccgcccg gaatgcccgt
    15421 cacaatctta atgccctgct gttccaggaa atgaacgata aattctgcgc cggtaaagcg
    15481 cttacgcgtc gatgttgtgc ccgaacttgc catgctccag tccttttctt ctgggccgac
    15541 tttccgggag gtgcttaaac gaaaaacccc gcccggtttg cgccggcggg gttttggaat
    15601 cgtgtgttgt tccagtccct acggcgcatt gccgacgacc accaccacac gcacgacgac
    15661 cactgcggcg gatggcgcag ttggtagtag ttttgcgttg aacatggaag tagtcattgg
    15721 ggaccttgtt ggttttgtgt ttaacaatat ttatacaagc acagctttac aggggagaca
    15781 atggaaaatt tttcagcaag ggaaaattga ggggttgatc acgttttgta ctgaattgca
    15841 gataacaaaa aaccccgccg gagagaggtt tcgtcagccg cctgcggctg gtaaccgcaa
    15901 agcacactgt attatgtcaa cactgaaagt atacgtgttc cgcgcagaac gcgcaatttc
    15961 ggcacgaatt ttgacgtatt tagtgcatag ttgagtatcg atcacagttt gcgttttgtc
    16021 caaatattac tgtttattta tacagtaaac ttctataata tcactgtacg caatgtgtta
    16081 tgcgggggcc gcatcgttac ccggcgcact aagtcctggc tgaaacgggt ggtgccgtca
    16141 gcgccttaac cccgcgtgag cacactgtgt tatgtcaaca agcacaacgt ttctccttga
    16201 gataccgcgt gcacaacagc tggcaacagg cagcggaaag gtacgtcagc tggcagtgct
    16261 cctgaaccac aggagacgcg tatgagcctg gtggatatcg ccattcttat cctcaaactc
    16321 attgttgcag cactgcaact gcttgatgct gttctgaaat acctgaagta attcagattc
    16381 aagtcgcacc aaaggggagc gggtaaccgc tcccctttta tatttagcgt gcgggttgga
    16441 gtcggatgcg atgctgacgc atcttatccg ccctaccatc cctcccggca acatttattg
    16501 ccgcttttgt ttacatattc tgccgctaaa caattcccca ttcctggcgt atatctggct
    16561 aacattcatc aatgtgatag attcctctcc cgcatttatg ggaatgcgta gtgacttatt
    16621 ctaattattt ttataaaagc atccgtgata atgaaaaggc aaagaaacgt caatttgtta
    16681 ttgatgttgg tattactcgt ggccgtcggt cagatggcgc aaaccattta tattccagct
    16741 attgccgata tggcgcgcga tctcaacgtc cgtgaagggg cggtgcagag cgtaatgggc
    16801 gcttatctgc tgacttacgg tgtctcacag ctgttttatg gcccgatatc cgaccgtgtc
    16861 ggtcgccgac cagtgatcct cgtcggaatg tccattttta tgctggcaac gctggtcgcg
    16921 gtcacgacct ccagtttgac ggtattgatt gccgccagcg cgatgcaggg gatgggcacc
    16981 ggcgttggcg gcgtaatggc gcgtactttg ccgcgtgatt tatatgaacg gacacagttg
    17041 cgccatgcta acagcctgtt aaacatgggg attctcgtca gtccgttgct cgcaccgcta
    17101 atcggcggtc tgctggatac gatgtggaac tggcgcgcct gttatctctt tttgttggtt
    17161 ctttgtgctg gtgtgacctt cagtatggcc cgctggatgc cggaaacgcg tccggtcgat
    17221 gcaccgcgca cgcgcctgct taccagttat aaaacgcttt tcggtaacag cggttttaac
    17281 tgttatttgc tgatgctgat tggcggtctg gccgggattg ctgcctttga agcctgctcc
    17341 ggcgtgctga tgggcgcggt gttagggctg agcagtatga cggtcagtat tttgtttatt
    17401 ctgccgattc cggcggcatt ttttggggca tggtttgccg ggcgtcctaa taaacgcttc
    17461 tcaacgttga tgtggcagtc ggttatctgc tgcctgctgg ctggcttgct gatgtggatc
    17521 cccgactggt ttggcgtgat gaatgtctgg acgctgctcg ttcccgccgc gctgttcttt
    17581 ttcggtgccg ggatgctgtt tccgctggcg accagcggcg cgatggagcc gttccccttc
    17641 ctggcgggca cggctggtgc gctggtcggc ggtctgcaaa acattggttc cggcgtgctg
    17701 gcgtcgctct ctgcgatgtt gccgcaaacc ggtcagggca gcctggggtt gttgatgacc
    17761 ttaatgggat tgttgatcgt gctgtgctgg ctgccgctgg cgacgcggat gtcgcatcag
    17821 gggcagcccg tttaagcgca cgtcaccgca gcatcgtcat cagctccatg ggagaacgat
    17881 gctgctttat cagatcacgc atcacccgca tatgcggtgc ggagtaagaa taaaacgcct
    17941 gatagcccgc acaaagcacg ctgtctggcg tctcctgccg agcgtgcggg cacaggcgat
    18001 gccagtcgca cgcctggcag tcacggcgcg gttgcgggtt ttcgctgcca caccattttt
    18061 gtaaggtggc gtcaaaaagc tcaatcgaga tacgactgat atctgcccgc acccaacact
    18121 caaacagggc aattaaaaat ttcccccaca gctctgctgt caccgaagcg gccgtcagat
    18181 cgccccggtc atcaaattca agatggggaa tgagctgtac ggagcggatg ccccgctgtt
    18241 tgaaattgtg ataaaaatcg gcaggttgca gagcatcctt gtactctaca accagcttat
    18301 gcctgtcctc ttcagcatca atgacctgac ttcctgtcat gcgactctct caataaatac
    18361 gataagttga tgtatatgcg ttacagcaaa cagtattgcg agagataaca ccgcgaggga
    18421 gatcaaaaat ttgtcacgga ctacgtgaaa ttgggaaaaa tcgctattcg tgacatccat
    18481 taaattgcga ttacgggtgt agtgccagag gatcagcgcc acgatggcga ggatgccaat
    18541 cgaaatccag aataacatgc ccgcctggtg ccagttgtgt ttgatagcca acgccatcaa
    18601 cgcgccgtag cccagcatgg tacgaaacca cgccagtgat gtgcgctccg gctgcagccc
    18661 tgggtcggca atgcgtctgg ctttacggct atccggcata caacaccagt cccataacga
    18721 tcaccgccac aaccatcaat attaagctga tgattaataa gctgttggtg tagggcaggt
    18781 cctctttcag acgcatcgct ttttcattgc gtaaccagcg caggtagcca tacattgcta
    18841 acccgccgga aaacagacac aacaacaacg ccagcagttc acgaatgacg ggcgtggcga
    18901 aatctggcgc aagttgatca agcccaaccc ccgccgccag aaatcctagt gcggtacgga
    18961 tccacgccag aaaagtacgc tcattagcca gcgagaagcg gtaatcgggc gcttctccaa
    19021 ggcgggaaat tttcattgtt gctcctttgc aatttcacta acgctctatc tttagcggtt
    19081 ttttttaaga atttcgtccg cccgtttcct gatatgcttt tgccatccgc tacaaggagg
    19141 tcagatgggt atcatagcgc aaaataaaat cagttcattg ggcatgctgt tcggtgccat
    19201 cgctttaatg atggggatta tccacttttc attcggccct ttttccgcac caccgccaac
    19261 gcttgaaagc atcgtggcgg acaaaacggc tgaaattaaa agaggtcttc tcgccggaat
    19321 aaaaggcgaa aaaattacca ctgtggagaa aaaagaggac atggatatcg ataaaatcct
    19381 cgatcaaagc ggtatcgcac tggccattgc tgcgctactt tgcgcattta ttggcggtat
    19441 gcgtaaggaa aatcgctggg gcattcgcgg cgcgctggtt tttggtatcg gcatcgtgtg
    19501 cagcattttg ctgatattcc tgattttttc cttcctgacc ggcggttcgc tggtttaagc
    19561 agcctgaata atgcccgccg ggtgatcatc agcgggcatt atcgtcagaa tttctgtttt
    19621 ttctcctcca ccttcactcc ttgcgtcggt aggccggtgt catagtcgcg taccactggc
    19681 gaatagccat cttgtgggcg tgggcgaaac gcccccatcc agcgcggtcg tgcatcttta
    19741 cgccacggac gcagactcca ttggtaactg cggaacggat cgcgaatttt gtccatgtaa
    19801 tccaataagg cgtcatgcat tttgctgcga acgtctgcaa aacggatatc atcgatcagg
    19861 ttatgcattt catttgggtc attacggcga tcgtaaagtt catcactggt gaagaggttg
    19921 agcaccagtt taaagtcatc cgtcacccag caacgcaccg gaataaaacc gccaaagctg
    19981 tcatgctcaa tctcgtagcg gttaaattcc accatcacgc cgcgtggctc tttcacggca
    20041 aggatatttt cccccggcag aatctctggt ttttcaatat ctgccagcgc catcattgtc
    20101 ggcagtaaat caatatgact gactggcgta tcgacctgtc gccgctcccc ttgcggcgaa
    20161 cggatgatca gcggaatacg ggtgatgtcg tcatacatcg ccgctccttt actgatcagc
    20221 ttatgtgcgc ccatcatttc accgtgatcg gaggtataaa tgacccacgt attttcacgt
    20281 tgctctgccg ttaaggcatt gatgacacgt ccgatttggt catcaacaaa gtcattgcag
    20341 gcaaaataga gcggatggtg ataaagcccg tcatcaccga ctggcgatgg catcgcctgc
    20401 gcccataagc ggtgatgttc cggtttgttc gccaggtcat cctgtgattt ctcgccaagt
    20461 tcgtagtaaa aatcagcgta tttctctaaa tactccaccg gacaggtgaa cgggtgatgc
    20521 ggctcatcat acgaaaccac catcaggaag ggttcctcgg cgcgcacggg ctgttgcaga
    20581 aaatctaccg ctcgattgct gatgcggtgc gcccaggtga aggtttcgtc aatatggttc
    20641 gcctgtaaat cttcgacgct gtttaggcca ttgcgccaca ggctgatctc tttttccgtc
    20701 agttcgctaa gatagttcgc cccatcaaac cagtaatcag catcccactc cggcggacac
    20761 tcgccagtgc cgaaatagtc atgaccgtcg agatgccatt tgccgatgta acaggtgtga
    20821 tagccggcat ccttaaagta gcgccccata g
//
LOCUS       DADLSA010000061        20848 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.061, whole genome shotgun sequence.
ACCESSION   DADLSA010000061 DADLSA010000000
VERSION     DADLSA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20848
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.061"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(48..782)
                     /gene="araH"
                     /locus_tag="JN691_002248"
                     /pseudo
     CDS             complement(48..782)
                     /gene="araH"
                     /locus_tag="JN691_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
     gene            complement(794..2308)
                     /locus_tag="JN691_002249"
     CDS             complement(794..2308)
                     /locus_tag="JN691_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HAY7243688.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(2378..3367)
                     /locus_tag="JN691_002250"
     CDS             complement(2378..3367)
                     /locus_tag="JN691_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7243689.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVETPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            4164..4667
                     /locus_tag="JN691_002251"
     CDS             4164..4667
                     /locus_tag="JN691_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAY7243690.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(4747..4998)
                     /locus_tag="JN691_002252"
     CDS             complement(4747..4998)
                     /locus_tag="JN691_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAY7243691.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(5113..5199)
                     /gene="azuC"
                     /locus_tag="JN691_002253"
     CDS             complement(5113..5199)
                     /gene="azuC"
                     /locus_tag="JN691_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAY7243692.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            5462..5785
                     /locus_tag="JN691_002254"
     CDS             5462..5785
                     /locus_tag="JN691_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243693.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            5956..6453
                     /gene="ftnA"
                     /locus_tag="JN691_002255"
     CDS             5956..6453
                     /gene="ftnA"
                     /locus_tag="JN691_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAY7243694.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(6490..6729)
                     /locus_tag="JN691_002256"
     CDS             complement(6490..6729)
                     /locus_tag="JN691_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAY7243695.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            6919..8130
                     /gene="tyrP"
                     /locus_tag="JN691_002257"
     CDS             6919..8130
                     /gene="tyrP"
                     /locus_tag="JN691_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAY7243696.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLATAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(8192..8857)
                     /gene="yecA"
                     /locus_tag="JN691_002258"
     CDS             complement(8192..8857)
                     /gene="yecA"
                     /locus_tag="JN691_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAY7243697.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(9053..9139)
                     /locus_tag="JN691_002259"
     tRNA            complement(9053..9139)
                     /locus_tag="JN691_002259"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9103..9105),aa:Leu,seq:taa)
     gene            complement(9152..9225)
                     /locus_tag="JN691_002260"
     tRNA            complement(9152..9225)
                     /locus_tag="JN691_002260"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9191..9193),aa:Cys,seq:gca)
     gene            complement(9280..9355)
                     /locus_tag="JN691_002261"
     tRNA            complement(9280..9355)
                     /locus_tag="JN691_002261"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9320..9322),aa:Gly,seq:gcc)
     gene            complement(9507..10055)
                     /gene="pgsA"
                     /locus_tag="JN691_002262"
     CDS             complement(9507..10055)
                     /gene="pgsA"
                     /locus_tag="JN691_002262"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAY7243698.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(10112..11944)
                     /gene="uvrC"
                     /locus_tag="JN691_002263"
     CDS             complement(10112..11944)
                     /gene="uvrC"
                     /locus_tag="JN691_002263"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAY7243699.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(11941..12597)
                     /gene="uvrY"
                     /locus_tag="JN691_002264"
     CDS             complement(11941..12597)
                     /gene="uvrY"
                     /locus_tag="JN691_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAY7243700.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            12893..13069
                     /locus_tag="JN691_002265"
     CDS             12893..13069
                     /locus_tag="JN691_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243701.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            13056..13280
                     /locus_tag="JN691_002266"
     CDS             13056..13280
                     /locus_tag="JN691_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAY7243702.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(13313..13933)
                     /gene="sdiA"
                     /locus_tag="JN691_002267"
                     /pseudo
     CDS             complement(13313..13933)
                     /gene="sdiA"
                     /locus_tag="JN691_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(14163..14915)
                     /gene="yecC"
                     /locus_tag="JN691_002268"
     CDS             complement(14163..14915)
                     /gene="yecC"
                     /locus_tag="JN691_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAY7243703.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(14912..15580)
                     /gene="tcyL"
                     /locus_tag="JN691_002269"
     CDS             complement(14912..15580)
                     /gene="tcyL"
                     /locus_tag="JN691_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAY7243704.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(15595..16581)
                     /gene="dcyD"
                     /locus_tag="JN691_002270"
     CDS             complement(15595..16581)
                     /gene="dcyD"
                     /locus_tag="JN691_002270"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAY7243705.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(16686..17486)
                     /gene="tcyJ"
                     /locus_tag="JN691_002271"
     CDS             complement(16686..17486)
                     /gene="tcyJ"
                     /locus_tag="JN691_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7243706.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(17574..18125)
                     /gene="fliZ"
                     /locus_tag="JN691_002272"
     CDS             complement(17574..18125)
                     /gene="fliZ"
                     /locus_tag="JN691_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAY7243707.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(18171..18890)
                     /locus_tag="JN691_002273"
     CDS             complement(18171..18890)
                     /locus_tag="JN691_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HAY7243708.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNEREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(19055..20707)
                     /locus_tag="JN691_002274"
                     /pseudo
     CDS             complement(19055..20707)
                     /locus_tag="JN691_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
BASE COUNT         5212 a         5366 c         4829 g         5441 t
ORIGIN      
        1 gcaaacatgg actggcaaaa gtgtagcggt attaagcgca gctatttagt catacgtgaa
       61 gccagaataa ttccggctat cgcagatacc aggccagaga gaacaaagat aataattttg
      121 gtgcgaacaa ccggtacacc cgccagacgc gcagcctctt cgttcccgcc aatcgccagg
      181 gtattacgac caaaggtggt tttattcagc agcaaaccaa agataatcag acacgcgacg
      241 gtgagccaga ttggcgcagg cagaccgaac cagttagcgt aaccaagggc aaagaagctt
      301 tcatcttcga taccgaccgc tttaccgtct gaaatgatgt acgccagacc tcgaacaatc
      361 tgcatcgttg ccagtgtcgt gatcagagca tttattttca gtttggcgat aacaaagcca
      421 ttgaccaggc cacagagaac gcccagcgac aaacccgctg ccacgccaat ccacaggctt
      481 tcagtcaggt tgataaccac cgccgtggtg acacccgcac aggcaattac ggaggcgaca
      541 gaaaggtcaa agtcaccgga agcaaggcag aacaacatgc cacacgccac catccccgac
      601 atggaaattg ccaggcccaa ccctttcata ttaatgaagg tggcaaaatt tgggacaaaa
      661 atggcacagg cgataaagag caccgcaaac accaccagca tgccgtactg atcccagata
      721 cgcccgaagc tgaatgacga cttaggtgcg ccagaccccg atgtagaaac agaagacatc
      781 atactctcct tactcaggca actgcctggc tgactttagg catcgcaagg ctcagtgcct
      841 gacgctcatc tgcctgctcg tgtaacaatt caccggcgat ttcgccttcc cgcatcacca
      901 caatccggtc ggcaacgccg aggacttcag gtaagtcgct ggaggcaaac agcaccgcca
      961 cgccctgcgc cgccagcgca taaattacgt tatatatttc gtgcttagcg ccaacatcaa
     1021 tgccgcgcgt aggttcatcc agcaaaatga ccttcatctc ttccgataac cagcggccca
     1081 gaatggcttt ttgctgattt ccgcctgaga gattcatgat cagttgctcc gcgcccggcg
     1141 ttttgatgtt gagcgaacga atgtgctgat cggcattgtt ttcttcccaa ctgttgttga
     1201 ttacacaacc gccgagcaca tgtttgcgtc tggcactgat gttgatattg tcgcgaacgg
     1261 agtgcacggg aataatgcct tccgctttgc gagcttccgg gcagagcatc atgcctgcgg
     1321 caatggcgtg gctcggttta cgaatatcga tcggctgttg gtcgatataa acctgaccgg
     1381 cggtgatttg cgtcccgcca aacaagcctt tcattaattc gctacgcccc gcccctacca
     1441 gaccaaacag cccaacaatt tcaccactgc gaaccgccag acttattggc gtacgcacgc
     1501 ctggtgcttt cacagcatca agacgcaggc gttcctcgcc ataactacga ggttgccagc
     1561 cgtagatatc gccaatgtcg cgcccgacca tcgcctgcac cagcgcgtcg tggtcaacct
     1621 gctgcatatc ggtaaaggtt ttgacataac gtccatcttt aaagacagtg atggcatcgc
     1681 tgagggcaaa tatttcttcc atacggtgag aaacgtataa gatcacccgc ccctcttttc
     1741 gcagttcacg aataacgcgg aaaagattgc cgatttcgcg ggcagaaagg gagctggttg
     1801 gctcatcaaa ggcgataatt ttagcgttac gcgccagcgc tttggcgatt tcaaccatct
     1861 gccactgacc aatggagaga tatttcagcg gcgtgtccgg gtcaatatcc ataccaagat
     1921 gtttaagttg taaacccgcc tcataattca gcaatgagcg attcacaatg ccgcctttat
     1981 gcggcagctg gccgagatag atgttttccg cgacggtcat ttccggcacg agatgcagtt
     2041 cctggtaaat aatcgccaca cccgcgttaa gtgctgcggt cgtgtcggaa aaggacattt
     2101 cctgcccatt aatcactaca gaacccgtgg ttggcgcata gttgccgctg aggattttta
     2161 agagagttga ttttcctgcg ccattttcac ccatcaacgc atgaacctga ccggcatagc
     2221 agtcaaaact aatatccgtc agcgccttaa cgccgggaaa cgttttaccg atgccgcgaa
     2281 atgagagata cggggtagac tgttgcataa cgtctccgtg aatcactggt cgttcacacc
     2341 ccctctgcat catgcagagg ggaatttttc cggcaaatta cttaccgcct aaaccttttt
     2401 tctccagttc ttctttaaag ttgtcacgcg tgatcagtac cacgtctgta acttcggtaa
     2461 attttggcgt ttcaacgtct tttgctaccc agttgtaaag catttcgctg gatttatagc
     2521 catgtacgtc cgggcttggc agcagggaac cgtagaagcc ggttgcctgt gctttagaca
     2581 gttcgcttac tgcatccaca ccgttaatac caataccgat gatatcggct gctttaaagc
     2641 cctgaccttc ggttgcacgt acgccgccaa gtacggtgct gtcgttcata ccgacgatca
     2701 gccaatgttt aacttccgga tgttgaacca gcattgagtt ggcagcgtca aatgcccccg
     2761 ggatgtcgtt agatttggta ggtacctgat aaatttgttt ttccgggaat cctgcagctt
     2821 tcagtgcgtc catagaaccc gttgtacggc ggcgggcggt atccagttcg ttggcggtaa
     2881 tcgccatcac tgcgctttct ttgacatccc agccacgttt ctgcatctct ttatacagtt
     2941 cctggccctg acgttcgcca attttagtcg ccgccatcat caccagagga acggtatcca
     3001 ttggcttacc tttggcgtta acaaactggt catccactgc aatgactttc atatcgtagc
     3061 cacgcgcttt cgcgacgatg gcagagccga gtttcgggtc cggagtacaa ataacgaaac
     3121 cttttgcgcc actggctgcc aggctgtcga tcgcgttcaa tgttttttcg ccatccggca
     3181 cggcaatctt aataacctca aaccctaaat ccttcccggc tttatcggca aacttccatt
     3241 cggtctggaa ccacggctct tccggttgct tcaccagaaa accgagcttc aggttctccg
     3301 ccatagcgga ttgtgacata acggctgcca gaccaatggc tgccagggct ttagtaaatt
     3361 tgtgcatggt tctctccagc tttagtgtcg ttttgtgtag ggcaaaaacg aatgacattc
     3421 gttaaattaa tcggaaaaca aagcattacc ttttatctaa aagataagtg accgtgttga
     3481 catagtttta gcgagaaatt aattctccat aggagagcaa tatcacatcg cagaattaca
     3541 gtgagaacgt gcataaattt agcgggaaaa gacataagga aaagccaatt tgtcagacaa
     3601 attgttgaat gcacagcaga ttaatccata agattagcct ggaaatcctt gttgtctttg
     3661 gtacccatgc gggatgtctt ctttttaacc agtcaatagg ccgcattacc tggcgttgag
     3721 tttttgaaat ggtgtaataa ccacaactca aagatgtgga aaatgcgcgt cattcatttc
     3781 gccattaatt atcactgtgc tcattaatta acagaacacg tataatgaga gccatctcgc
     3841 aaaaatgaaa aaacgtttta taaaatcatc acttcatcat gaattcaaat tcatcgatta
     3901 atatcaacaa gatacaaaaa gcactatcat taaaattcat tgcatttaca ttgatttcac
     3961 caatgaaatg taaaaatata taaacttgat gatttaagca ttttcttata accgttcaga
     4021 cgttattctt atttcagatc atcgtcagaa ttgactccac gatcacattt cggaccggca
     4081 gaaaggaatt attctgcaaa cagtaattat ggtgttttga tttatcttgc acctctccac
     4141 ttctggatat aaggatatta ggtatggcaa ccgctggaat gcttctcaaa ctcaactctc
     4201 aaatgaaccg cgagttttac gcatccaatc tctaccttca cctgagtaac tggtgttctg
     4261 aacagagtct gaacggcacc gccactttcc ttcgcgccca ggcacagagt aatgtgaccc
     4321 aaatgatgcg catgtttaac tttatgaaga gtgtcggcgc tacccccatc gttaaagcca
     4381 ttgatgttcc aggtgaaaaa ctgaactctc tggaagaact gttccaaaaa acgatggaag
     4441 aatacgagca acgttcaagc acgctggcgc agttagccga tgaagcgaaa gaactgaatg
     4501 atgattcaac cgtcaatttc ctgcgcgatc tggaaaaaga acagcagcat gatggcctgt
     4561 tgctgcaaac cattcttgat gaagtgcgca gtgcgaaact tgcgggtatg tgccctgtgc
     4621 agaccgacca acatgttctg aatgtcgtgt cacaccagct gcattgatca tacatcggcg
     4681 ctaatacatt gcgccgatga agtttttgag aaaccgctgc ctcatctgtt tgaagcagcg
     4741 gtttttttaa tgggattcac cctgtggggt aaacttgagt tcaatcagcg cgatggcttt
     4801 ttggattgcc cgcatggtga ccgggtctgc ggcagcggga tgatgagtaa agtcgatatt
     4861 cttcagctga ctggacattt tttcacgaac ttcaacgggc gcgattacat cgagaacatc
     4921 cagaatttgt ttgataacca actggcaagc taccacatca gaaaccagtt cctgatcggc
     4981 attcagcggc tgggacatcg taaactcctg atagcatttt gaaagccgtt atagtagcgg
     5041 cttcacatct tcagcgatag tcacatccac cgtcatcagg acacaaaaaa acctgccgga
     5101 gcaggttttt tgttatcgga acatattgcc tggcggtacg tctttgaacg tcttgcaata
     5161 gttattgaac atacttttca ggattttgcg cagtttcatc gcggcactcc gaccatttgt
     5221 tatacaggag ttattgtctt tgcgcttata atatgacaac catcacaaaa atcaatcttt
     5281 atgtgataca aatcacataa ataccccttt aatgttataa aaatgataat caaaaaacag
     5341 cccccctatt tctgacacct acagatggca agaaatagcg cctgccaggc gtcttttccg
     5401 gccattgtcg cagcgctgta acgcgtaaaa tagtgctttc tcttactctt ctggctggac
     5461 catgagactt ctgattctga ctctttcatt aataacgctg gcggggtgta cggttactcg
     5521 ccaggcccat gtgagcgaag ttgacgcggc aacaggtatt gtacggttgg tttatgacca
     5581 ggcttttttg cagcatgccc ataccgatcg ttatgtcagt cgcgggatcg ccgatcgcgc
     5641 atgccagcag gaaggctata cccacgcgat cccctttggt cagccagtag gcaactgtag
     5701 cctttttgcg ggttccttat gtttgaatac ggaattcact ttgtcatatc agtgccatca
     5761 ttccgccttc cctgtctttc tctaaatatt ttaatcagcg aggggatctt cgctgattaa
     5821 agaaatagcg gtaatgcgtt ttattcccat tcgtattttt aataattgaa gtttatattt
     5881 taccttttgc aaataataaa ataacaaatt atagtgacgc cacagcaaca aatataacct
     5941 ttgtggagca ctatcatgct gaaaccagaa atgattgaaa aacttaatga gcagatgaac
     6001 ctggaactgt actcttcact gctttatcag caaatgagcg cctggtgcag ctatcatacc
     6061 ttcgaaggtg ctgccgcgtt cctgcgccgt cacgcccagg aagagatgac gcatatgcag
     6121 cgtctgtttg attacctgac tgataccggc aatttaccgc gtattaatac cgttgaatct
     6181 ccgtttgctg aatattcctc acttgatgaa ttattccagg aaacctataa acacgaacaa
     6241 ttaatcaccc agaaaattaa cgaactggct catgctgcaa tgaccaatca ggactaccca
     6301 acatttaatt tcctgcagtg gtatgtttct gagcagcatg aagaagagaa actgttcaaa
     6361 tcgattattg ataaattaag cctggcaggc aaaagcggcg aaggtctgta ttttatcgac
     6421 aaagaactct ctaccctcga cacacaaaac taatgctctc cggcggcagg tttctgccgc
     6481 cgtttcaaat cagtgacggc aaatcttact ctgatcggtc gaaaaacctt cttctgaagg
     6541 tataaatttg ccttttgctg ccagaaacgc taccagctct cccgctgtca tcccttctgc
     6601 cgagcacgtg tgaaaacgtg cctgttcacc aaaacgcgct ttaatcgcag cttccagact
     6661 ggcatgcgta tattgctcgc ctgattcaat catcatattt aacacttcat gaccgtgaat
     6721 agagtccatc gttcctcctc aaaaaaagcc tagcgtagcg attacctctt atgaagactt
     6781 tgcgccagcg caggactgaa tgcttttatt gtacatttat atttacacaa tatgtaacgt
     6841 cggtttgacg aagcagccgt tatgccttaa cctgcgccgc agatatcact cataaagatc
     6901 gtcaggacag aagaaagcgt gaaaaacaga accctgggaa gtgtttttat cgtggcggga
     6961 accacaattg gcgcaggcat gctggcaatg ccgctggcta cggccggtgt tggttttagc
     7021 gttacgttaa tcttgttgat tgggctttgg gcgttgatgt gctacacggc gctattactg
     7081 ctggaggtgt accagcatgt tccggcagat accggtctgg gcacgctggc aaaacgctat
     7141 ctgggacgct acggtcaatg gctgacgggc ttcagtatga tgttcttaat gtatgctctg
     7201 actgcggcat acatcagcgg tgccggtgaa ttgttggcct ccagcatcag cgactggaca
     7261 ggtatttcaa tgtcggcaac cgctggcgtg ctgttgttca cttttgttgc cggtggcgtg
     7321 gtttgtgttg gaacatcgct ggtcgattta tttaaccgtt ttctgttcag cgccaaaatt
     7381 atttttctgg tggtgatgct ggtattgctg ctaccgcata ttcacaaagt gaatctttta
     7441 accctgccgt tgcaacaggg gctggcactg tctgcaatcc cggtgatttt tacgtcgttt
     7501 ggttttcacg gtagcgtgcc gagtattgtc agctatatgg atggcaacat tcgtaagcta
     7561 cgctgggtgt ttataatcgg tagtgccatc cccctggtgg catatatttt ctggcaggtg
     7621 gcgacgcttg gcagcattga ttcaacaacc tttatgggat tgctggctaa tcatgctgga
     7681 ttaaacgggc tgttacaggc gttacgcgaa atggtggcct ctccgcatgt tgagctggcg
     7741 gtacatttat ttgctgattt agccctcgcc acgtcatttc tcggcgttgc gttaggctta
     7801 tttgattatc tggctgattt gtttcagcgt tcaaataccg ttggtggacg attgcaaact
     7861 ggtgcaatta cctttctgcc gccgttggcg tttgcactgt tttatccacg aggatttgtg
     7921 atggcgctgg gttacgccgg tgtggcgctg gcggtactgg cattgattat cccttcacta
     7981 ttgacctggc aaagcagaaa gcacaatcct caggcgggtt accgggtcaa aggtggtcgt
     8041 ccggcgctgg tggtggtgtt tctctgtggt attgctgtga ttggcgtgca atttttgatt
     8101 gcggcagggt tgttaccaga agtggggtga tcagatagcc tcaaattcct tattgggtgc
     8161 cagaattaac gctgacaccc aatgtgacct cttaatgcag gcagcactgc ttaaatttct
     8221 taccactgcc gcacggacaa ggatcgttac gccccggttt ctcttctgct ttgatcggtt
     8281 gctgaacagc tttttcctgc ggatgcgcca tccagtatgc atgtaaatca agcgccgcca
     8341 gtcgaatggc atctacgctc tcttcaaacg cttctggcga cattttctct acccgctcga
     8401 agttttcctc agtaccgtgc agcgcaatcg cctccagcgc tggttttaaa gaatcgggca
     8461 acgttgacca gtcagaaagt gccacgcccc gcatatagcc aaagcaccac tcctcaacaa
     8521 tcgtcagctc gctgccatca acttctcgca agccgaataa cggctcaaac tgctccggga
     8581 attcgttcag acgctctgcg gtatcggcca tatgttgaaa agccaggttc ataaagcgcg
     8641 tcatctcttt ctctgacgcc cagcgcggca catagtcagc cccaccccac acggcaacca
     8701 gccactgttc tggttcaatc tcttgcggag aactcaacac cgccgtcaat aaaccgtcca
     8761 gctccgccac atcaaggatg gcgtggtcag tgttgtattt ggtcagaata tcgtccagcc
     8821 attccaactc actttcgttt aacggtcccg ttttcatacg cttttccttg tggatctcaa
     8881 ctcgccagca cctatcttac atgccggtcc gtatcagaga tactttttga gtggctttgc
     8941 cggtgattaa aaattaagga gggtgtaacg acaagttgca ggcacaaaaa aaccacccga
     9001 aggtggtttc acgacactgc ttattacttt gattttattc ttatctttcc catggtaccc
     9061 ggagcgggac ttgaacccgc acagcgcgaa cgccgaggga ttttaaatcc cttgtgtcta
     9121 ccgattccac catccgggct cgggaagaaa gtggaggcgc gttccggagt cgaaccggac
     9181 tagacggatt tgcaatccgc tacataaccg ctttgttaac gcgccaaatt cttcaggcct
     9241 ttcagccaga catccgcttg acgccgatgt cttttaaact ggagcgggaa acgagactcg
     9301 aactcgagac cccgaccttg gcaaggtcgt gctctaccaa ctgagctatt cccgcattca
     9361 tcaagcaatc agttaatcac ttgattttat tatcgtctgg caatcagtgc cgccgttcga
     9421 tgcgttgcat tctacttacc tggcgcgatg agtcaacgat atttttcacc acttttgatc
     9481 gtttgctgaa aattacgccg aaacgatcac tgatcaagca aatctgcacg cgcagcgctc
     9541 aaatattgca acattgacca cagagtcagt accgcagcca caaagaaaag tgcaataccg
     9601 gcgtactcaa cccaaatgtt cggacgccac agcagccagg ccaacgccac catctgggca
     9661 gtggttttca ctttcccaat ccaggagacg gccacgctac tgcgtttacc caactccgcc
     9721 atccattcgc gtagcgcaga aataataatt tcacgggcga tcatcgttgc cgccggtaat
     9781 gtcacccacc agctgtgata atgctcggtt accagcacca tggcgatagc cacgagaact
     9841 ttatctgcca cagggtcaag gaaagcacca aaccgggtac tctggttcca gcggcgtgcc
     9901 agaaaaccat cgaaccagtc agtcaccgcc gcgacgcaga aaatgagcgc ggcggcaaac
     9961 ggcgaccagg tgacaggcag ataaaagacc aatacaaaga atgggataag gatgacacgg
    10021 aacagtgtaa gcaacgtagg gatattaaat tgcataatga cgggtaacta tctgttgtca
    10081 gtaagattac ccctatgttg ctacagagac atcaatgttt caacgaccag aagatctttt
    10141 ctgccagacc ttgcgaaata cccggcactt ttgcaatttc ctcgacgctg gcgttacgta
    10201 aaccttgcaa accgcccata tatttcaaca acatttgccg acgttttggc ccgacgcctt
    10261 caatggtttc cagggaactg gtatttttga ccttcgcccg ttttttacgg tgcccgccaa
    10321 tcgcgtgatc atgtgattca tcgcgaatat gctggataac atgcagcgcg ggtgaatctg
    10381 gcggcaaact aaatccctca ccttccggct caaagaacag cgtttccagt ccagccttac
    10441 gatctgctcc tttggcaacg ccaagtagca gcggatgatt tttatcccat gagacatcca
    10501 gttcggcgaa gacatttttc gcctgcgcaa gctggccttt gccgccgtcg ataaggatca
    10561 catccgggat cttactgtcg tcaatggctt taccataacg ccgacgcagc acctgattca
    10621 tcgccgcata atcatcgccc ggcgtgatgc ctgtaatgtt atagcgccga tactccgcac
    10681 gcagcgggcc gttagcatca aacaccacac aggaagcgac ggtttgttcg cccatggtat
    10741 ggctgatgtc aaagcactcc atccgcttca cttccggcaa tttcaacacg ctggcaagcg
    10801 cggtcagtcg ctggtgaacg gtagattgct gcgaaagttt gctggttaag gccgtcgccg
    10861 cattggtgcg cgcgagtttc agataacgcg ccctatcgcc gcgaggtttg gtttgaacat
    10921 taatcttgcg tcccgccagt tctgaaaggg aatcggcgag cagcgtttta tcgctaagat
    10981 taaaatcgag caggatctca cccggtaagg tgcgcatctg gctgccttgt aaatagaact
    11041 gacctacgaa ggtttctacc acctcgctca gttccgtacc gccaggcact ttcgggaaat
    11101 agctgcggct gccgagcact ttgccctgac gaatgaacaa tacgtggaca caagccatgc
    11161 ccgcatcgaa cgccacacca ataacgtcaa ggtcgtcgcc ggtattggaa acaaactgtt
    11221 tttcggtgac gcgtcgcacc gcctgaattt ggtcgcgaat acatgccgct tcttcaaact
    11281 ccagattctg gctggcagtt tccatacggc taatgagttg cgtaagcacc tgatcatctt
    11341 tgccagacaa aaacaggcgc acatactcga cctgctgagc gtattcttct tcactcacca
    11401 gtccttcaac gcacggtccc agacagcgcc ctatctggta ttgcagacac ggacgcgagc
    11461 gattgcgata aacactattt tcgcactggc gaatggggaa aatcttttgc agtagcgcca
    11521 gtgtttcacg tacggcatag ccattcggga acgggccgaa atattcacct ttggcatgct
    11581 ttgcaccacg atgcatcgcc agacgcgggt gggtatcgcc actcaggaag ataaaaggat
    11641 aagatttatc atcgcgtagc aaaacgttgt aacgcggctg atagagtttg atgtagttgt
    11701 gttccagcaa cagcgcttcg gtttctgtat gcgtaaccgt tacatcaatt tgctggatct
    11761 gggcgaccag cgcttcggtt ttgcgcgaag cgaggttgct gcggaaatag ctggaaagcc
    11821 gttttttcag gtctttcgct ttgccgacat agataaccgt accaccagca tcgtacatgc
    11881 gataaacgcc tggctggctg gttacggttt ttaaaaacgc ttttgcgtca aactgatcac
    11941 tcactgactt gataatgtct ccgcattaca cagaccatgg cgaattgcca ggtgagtcag
    12001 ctcaacatcg ccatgaatgt ttagtttact gaacatacga tagcggtagc tgttcaccgt
    12061 tttcggactg agattgagct gttctgagat ctcattgacc ttctggccct tagtgatcat
    12121 cagcataatc tgcaattcac gttcagacaa actggcaaat gggctttctg ttttttctgg
    12181 ttcgatctgg cttaacgcca tttgttgagc gatatcagaa gcaatgtaac gctgcccaga
    12241 atagacagaa cgaatcgcac tcacgacttc ctgcggagcc gcgcctttgc tgaggtagcc
    12301 agcagcacct gcctgcatga ctttcgctgg taaagggttt tctgtatgga ccgtaagcat
    12361 gatgatttta acatcagctg tggaacgcgc gattttacgc gtcgcctcaa gaccgccaat
    12421 gcccggcata ctcatgtcca ttagcaccac gtcaacggca tttgtccggc accacttaac
    12481 ggcgtcttca ccgcacgatg cctctccgac gacttttata ccctttatat cttccagaat
    12541 gcgtcgtatc cctgcgcgca ccagttcgtg gtcatcaaca agtagaacgt tgatcaaagg
    12601 aatatctcca gaaataggga taacgctact gatagttagt cattcgtata ttagcggttt
    12661 ttattgcaac tttgaaacgt taaaaatggt gggtattcga tttttctctc gtttttgtca
    12721 attcgattgt ccacagttat ggtcacgccc gtcaccaacg gcttaccgga tcgtttcaca
    12781 gtaagcccta cgatgaaatg tgacaaaaat tgactttatt cagcaaaaat gaaaatcagc
    12841 ccgctgaata tgtaacatta attaacccga acaatacggg caaaaaacat tcatgataaa
    12901 aatatttatt ggtcattata ttaacgtatt ttatagcact gccgatatca cgctcaaaaa
    12961 acaaccactg ctatttttag caaagcttat ggtatactcc gccgccttaa catttttcac
    13021 cgcaaatttt cattgcaaca tgacgaggaa aataaatgag tacgcctgat ttttctactg
    13081 ccgagaataa tcaagaactg gcaaatgaag tctcctgcct gaaagcgatg ctgacgctga
    13141 tgctgcaggc gatgggacaa gctgacgcgg gccgcgtgat gttaaagatg gaaaaacagc
    13201 ttgcgctgat cgaagacgaa acccaggctg cagtattttc caaaacggtt aagcaaatta
    13261 aacaagccta ccgtcagtaa tataaaaccg gctgagagcg tgcctttcag ccggttattg
    13321 acaaaatcat cgctatctct gctgatgtct tcccttccgc cgtccatttc agaatttctt
    13381 tttcgcgctt gctgaaattc atctctggcg tcatcactat ttcatcattt aaacgcatca
    13441 gagccatcag actttcgcgc accagtaact gcatttttaa ttgcagttca tcactaagaa
    13501 tgggtatttc gcgcgtgctg caacgggaaa aggacaaaaa gcccagcgcc cgatttggca
    13561 gcattaaata ctgagtgaca ccgcggcgta aaccatgtgc gtgcgcggct tcccataatg
    13621 gctgtgcttc gctgaataag tcatcattcc acattaaatg gccctgacta aagttttcag
    13681 ggttcagcac aggatcaatt gcgagaaaat tttttgcctg ataataacta acccacgact
    13741 cagggtaatt ggtgtagaaa gccactttag gtcgcgtgaa tggtaccggg tggcgaacac
    13801 ataacgaata gtaatcgtac tccagctgct gagcctgaag ctctatttca tggtagacct
    13861 cttctgcggc ctccatcctc tgaaaacgca acagcatcgt gcgacgccag ctgaaaaaat
    13921 ccgtatcctg catagtaaac cgcaacgccc ctgagagtga gtatcattta taatgataat
    13981 tcaaactatc acataaataa gatttatata taacttatat tattcaggca atgaattact
    14041 tttgcaatcc atcgcattct cttatgttat taatgagtta tgctgatttg ttaagcagtt
    14101 ttatcacgct tgaaatggcg tcccgccccg acaggtgaat cgtcggggct gattttttct
    14161 tattattgca gcagaagctt ctcgaggaac tggcgggtgc gaggctgctg gggatcggca
    14221 aataacgctt ttgctgcccc ctgctcgact atccgcccct ggtccataaa gatcgcccgg
    14281 tccgcaacat cccgggcaaa gctcatttcg tgtgtcacaa tcaccatcgt gcgcttttcc
    14341 tgcgccagct ggcggatggt gttcaggact tcgcccacca gctctggatc cagcgccgac
    14401 gttggctcgt caaacaaaat cacctcagga cgcattgcca gtgcacgcgc aatcgcaaca
    14461 cgctgctgtt gaccgccaga caaacgacgt ggatagctgg tttctttacc ggccagccca
    14521 actttcgcca gcagctcgcg agcgcgcgcc gtggcctctt ctttcggttc gcctttgacg
    14581 atcaccggcc cttcaataat gttttccagc accgtacgat gcggaaacaa attaaagttc
    14641 tggaagacaa acccgacgtg ctgacgcaac tggcgaatca gcgatttttg ctgacttaat
    14701 gaacgtgcag tatcaatagt gatatcgcca acggtgatcg ttcccgcttc cggttgttcc
    14761 agcagattta tgctacgtag caatgtggtt ttgccggaac cactcggacc gataattgcc
    14821 accacttcgc caggctttac ctcgaggtcg ataccgtgca gcaccgtctg accgtggaat
    14881 tttttcacca ggttcttaac ttcaatggca ctcattttgg ttctctctcc tggcgattaa
    14941 gttgattctc aaaatggttc tgcaacgtcg ataacacagt cgccatgatc cagtagatga
    15001 gcgaagccgc caggtacatg gtgaacacct ccagcgtgcg cgaggtaatc aactgcgcct
    15061 gacggaacag ctccggcacc tggattgtcg cggccagcga cgtatctttt accagactga
    15121 taaagctgtt cgacagcggc ggcaacgcca cccgcgccgc ctgtggcaaa atcgcgcgac
    15181 gcatggtctg ccacggcgtc ataccaatac tggcagcggc ttcccactga cctttatcaa
    15241 tagaagaaat ggcggcgcgc agcgtttcgg cggcataggc ggcagtgttc agcgacagac
    15301 caatcattgc tgacggaatg ggatctaatt cgataccaaa ctgcggcagg ccgtaataga
    15361 tcataaacag ttgtgcgatg agcggcgtac cacgaaaaat agagatataa aaacgcgcca
    15421 gccagcgcac gggccagatt ggcgacaggc gcatcagcgc gagaataaac ccgagtagta
    15481 agccaaaaaa catgccgccg atactgagtt gcagggtata cccagcccct ttcaacagga
    15541 acggcaagga atcaataacc agttgtatac tttcttgcat gagcgttccc ggagctaaac
    15601 gtggggatga taggcgaaca gcgcaggcgc gccgccggta tgaataaaca gaatcggccc
    15661 ttcatctttg aagcgtttct gactgatacc gtcaatcagc cccgccatcg cttttccggt
    15721 atacacagga tcaagcagaa tgccttccag acgcgccagc aatttcactg cttccatgcc
    15781 ttcgtcgttc ggcacgccgt agccaggtgc aaaatagtca tcccagagta aaatttccgc
    15841 tgatgcggtc agctccagtt ctttcgcaat cgcctgttgt aggttaacca ctttcggcaa
    15901 ttgatcggca acggaacgcg acacggtcac gccaatcagt tcgctttcag gcatcaggtg
    15961 ttccagccca acagccagtc cggcgtgagt tccggcactg cccgatgcga ctaccaccga
    16021 cgaaatatta accgcccctt cacactgttg cgcgatttcc agcgcactct ccacataacc
    16081 tagcgcgcca agagcattag aaccgccaac cggaatgaca tacgggcgaa agccttgtgc
    16141 ttcgactcgc gtcgccagct cttccagttg ggcattggga tcggtcagtg cgtcgcacat
    16201 ttcaatctgg gtattgaaca gatccagcaa caaacgattg ccgttggtta aatagttttc
    16261 tgcggttgtg ccaataggat tttccagcag cgccacgcag tgcagaccga gtttcgccgc
    16321 gactgcggca gtctggcgca catggttaga ctggatcgcg ccggcagtaa tcagcgtatc
    16381 ggcaccttcg cgcagggcat ctgccgcgag aaattccagc ttacgtaatt tattgccgcc
    16441 cattgccatg ggggtgacgt catcccgttt gatgaaaatt tcccgtccaa gataatcaga
    16501 aaagcgcggc agatattcga gcggcgttgg cgcgccgata aactccaacc gtggaaaacg
    16561 ggttaaatta tgcagtggca taacagcctc cgatgtgtgt tgttgtgatt ttcttattat
    16621 gcacgctgaa aacgcgtaaa taaaaaaggc gctagtgaaa gcgccctttt ttgtcattat
    16681 gctgattatt tggtcacatc agcaccaaac catttttcgg aaagagcttg cagagtgccg
    16741 tctttttgca tttccgcaat tgcatcattc accgctttca gcaggtcttc atttccttta
    16801 cgcagcgcca cgccagactc ctgacgggag aatgcttctc cggttactgc cagcgtatcg
    16861 ttggttttct tcaccagatc cagcgccgcc agacgatcaa cgaggatcgc atcgatacgc
    16921 cctacgcgca gatcctgata tttggtcggg tcatcatcat aggtacgcac atcgacaccc
    16981 tgaacattct gccgcagcca ctcttcatag ttggtgccca gaccgacacc cacttttttg
    17041 cctttcagat cagcagccgt tttaatggtg ccttcgttac cttttttcac cagcgcctga
    17101 ataccggaaa tggtgtaagg ggtagagaaa tcgtattttt tcttgcgctc gtcagaaatg
    17161 gtgacctgat taatcaccac atcaatgcgt ttagagtcca gcgacgccag cataccgtcc
    17221 catttggtcg gttttagtga cgcctcaacg ccaagatgtt ttgccagctg ttgggcaaat
    17281 tccacttcaa aaccggttaa tttgccgtca tccccctgaa aactgaacgg cggataagtg
    17341 ccttccagcc ctaccagcag cgtgccgcgc tctttgactt tattaagcag accttcatct
    17401 gcaaaacttt taacgctcat gcccgcaacc agcgccacgg ccatcacacc catcaatgcc
    17461 tgacgtccca gatgtgctaa tttcatattc accccgaatg ttgttatgtc tgtttgcagt
    17521 gtagagccat tgtttgtaaa cacaaaaaca actccgctac atcttattct tatttaatat
    17581 atatcagaag caggcaggct ggaggatttc tgcacaagtc cggtacaggt ttggcgctga
    17641 tagtgttgat acttccgtaa tgcgatgcgg taattgttgg tgctggtggt gggcagccac
    17701 ggggcgagga tttcatcaag gaaaccgtcc tgcagctgat cgagggaaat attttgctca
    17761 tgtagatgat tacccaaacg gcgcagacga acgacatatt cacgcaccgt acctgggctc
    17821 atttcagttt gctcaaaaag aaatttctta aagccgataa tatcgaaaaa atcactctgc
    17881 gttttgcaat gtaaatcacc gcaaaatcgg cacaatgccg cccatgattg ttgttccgtt
    17941 tgccagcctt tttcatcaag cagcgtgtcc aggcgggaaa tctcgatttt attcactatt
    18001 ttgccgtcgc gaactaaggt aatgcgatcg agtaatttac ggcaatgcgc gcaatgggtc
    18061 tggctgtgtt taaagtcttt aagatagcgg cttaatggcc gtcttttcag gtgctgcacc
    18121 atcattaaga actcctggta gtcaaagtta aagtgcggca tttactgacg ttataactta
    18181 cccagtttag tgcgtaaccg tttaatggcc tggctgtgta actgactgac ccgcgattcc
    18241 ccgacctcca gcaccgcgcc aatctctttg agattcagct cttcctggta atagagggtt
    18301 aataccagtt tttcgcgctc cggcaacgtt tcgatggctt ccatcacccg ctggcgcaga
    18361 ttactgtcca gcagttgttg tagcgggttt tctcgctgat gatcatcagt aaccagttcg
    18421 atgctatcgc cgtgctcttc acgccactca tcgtaggaga agagctggct gttattggtg
    18481 tcgagcagca tttgacgata atcggcaata tcgatcccta aacgttccgc tacctcagtt
    18541 tccgtggcgt tgcggccaag ttcctgctcc agttgcccta ttgcctgtgc cacttcacgc
    18601 tcgttgcgtc gcacgctgcg cggcacccag tcacggctgc gaagttcatc cagcatagcg
    18661 ccacggatac gctgcactgc gtaagttgta aatgccgttc cttgtagggc gtcatagcgt
    18721 tcgacagcat taagtaaccc aatgccgccc gcctgtagca gatcgtcaag ttccacgctc
    18781 gcgggcagtc gaacctgcag gcgcaatgct tcgtgacgca ccagcgggac ataacgctgc
    18841 cacagcgagt gtttatccat tacaccttca gcggtataga gtgaattcac gataaacagc
    18901 cctgcgttat atgagttatc ggcatgatta tccgtttctg cagggttttt aatcggacga
    18961 ttagtgggtg aaatgagggg ttatttgggg gttacgcaaa aagccgaatg taaaaaaccc
    19021 cgccggtggc ggggtttgag aggcattcag acgattaacc ctgcagcaga gacagaacct
    19081 gctgcggtac ctggttggct tttgccagca cggagttacc ggcctgctgg ataatctgcg
    19141 ctttcgacat gttggacact tcggtcgcat agtcggcgtc ctgaatacgg gactgcgctt
    19201 cagacaggtt ggtagtggtg ttgttcaggt tggtgactgc ggaatccaaa cggttttgca
    19261 ccgcacccag ggaggagcgg aatttgtcga tggagctgat ggcttcatcc agagctttca
    19321 ggggatcggc tgtcgtagct gcttttgttt cagcatctgt cgtcaattta ccatcagcat
    19381 ctttataaac tgccttaccg ctgccgttag tcactgaacc atcatcctgt aggtaaaaat
    19441 ctacagtatc agatgtggtc aacgccccgt tgttatcaac ataaaccgaa tcaacacctt
    19501 ttgttacagc gccagttgtg ccgttgacgc tataacctgc agtagtgaaa tcaacattgt
    19561 ttcccgaggt tgatacagct ttatcgaaag ctgcctaatc aacttttgca cctgttactg
    19621 aataagtaat agtgtccggc gtagtactat taccggtaaa ggagatagag gtaccgccaa
    19681 tgtcaatggt tgtcgctttg gtattattgg ctgcaagtgt ggacagacta gcctcagtca
    19741 aagaagcacc agagccgttt ttcgttaagc gcccagttgt atcaatgtag agtttatctc
    19801 cattgcttgc cgtaatttta ccatcactgc tgatctgaac atcctgtgca gaaccatcga
    19861 tagtaatagt gcccttagcg gtatcaccaa ctcccggggt caaatatttc tgaacatcgg
    19921 cagctgaagc cgttgtggta tcaaaagagt atgacttact tgcactgtca tacttgtaat
    19981 tcgttgcact agccgccgtt ccgaagccgt tattaatggt tgcctgaaca gtatcaccat
    20041 cactaactcc agccagcaaa tcagacgctt tagcagcatc gtaacccgca ctcacagtat
    20101 atttgttgcc gaccactgtt gcattagcag ctaccaagtc agctttagat gcagcagtat
    20161 tagccacagc cccgccacca ttcacattaa acccattcag ccccagcgta tcagagtcaa
    20221 ttttcttcag atcaatagtg atagtctggc cgtcattcgc accaacctga attttcatcg
    20281 aaccgtcttt tgccagtacg ttcacgccgt tgaactgggt ctgaccggat acgcggtcaa
    20341 tttcgtcgag acgggatttg atttcgtcct gaatggagtc cagatccgaa tcagagttag
    20401 tcccggtaga agcctgaacc gtcagctcac ggatacgctg taagttgttg ttgatttcgg
    20461 acagcgcgcc ttcagtggtc tgtgcaacag aaataccgtc gttggcgtta cgtgcagcct
    20521 gagtcaggcc tttaatgtta gaagtaaaac ggttagcaat cgcctgaccc gcggcgtcat
    20581 ccttcgcgct gttaatacgc aagccagaag acagacgctc gatagaactc gacagcgcag
    20641 actggttctt gttgatatta ttttgagtga tcagcgagag gctgttggta ttaatgactt
    20701 gtgccatgat tcgttatcct atattgcaag tcgttgatta cgttttgggt ttccacccgt
    20761 cggctcaatc gccgtcaacc ctgttatcgt ctgtcgtaaa acaaccttta gaattttttt
    20821 cacaaacagc cattttttgt tagtcgac
//
LOCUS       DADLSA010000062        20684 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.062, whole genome shotgun sequence.
ACCESSION   DADLSA010000062 DADLSA010000000
VERSION     DADLSA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.062"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..69)
                     /locus_tag="JN691_002275"
     CDS             complement(<1..69)
                     /locus_tag="JN691_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7243709.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRR"
     gene            77..577
                     /gene="tnpA"
                     /locus_tag="JN691_002276"
     CDS             77..577
                     /gene="tnpA"
                     /locus_tag="JN691_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY7243710.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(616..963)
                     /gene="grxD"
                     /locus_tag="JN691_002277"
     CDS             complement(616..963)
                     /gene="grxD"
                     /locus_tag="JN691_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAY7243711.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1298..2125
                     /locus_tag="JN691_002278"
     CDS             1298..2125
                     /locus_tag="JN691_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAY7243712.1"
                     /translation="MARINRISITLCALLFTTLHLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEIYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2253..2834
                     /gene="sodB"
                     /locus_tag="JN691_002279"
     CDS             2253..2834
                     /gene="sodB"
                     /locus_tag="JN691_002279"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAY7243713.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2980..4149)
                     /locus_tag="JN691_002280"
     CDS             complement(2980..4149)
                     /locus_tag="JN691_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7243714.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGSPLMTLLLSHRARRSALIFLMAIFTPGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFLGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4315..4404)
                     /gene="ynhF"
                     /locus_tag="JN691_002281"
     CDS             complement(4315..4404)
                     /gene="ynhF"
                     /locus_tag="JN691_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAY7243715.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            4703..5728
                     /gene="purR"
                     /locus_tag="JN691_002282"
     CDS             4703..5728
                     /gene="purR"
                     /locus_tag="JN691_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAY7243716.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5725..6657)
                     /locus_tag="JN691_002283"
     CDS             complement(5725..6657)
                     /locus_tag="JN691_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7243717.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            6770..7981
                     /locus_tag="JN691_002284"
     CDS             6770..7981
                     /locus_tag="JN691_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAY7243718.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8272..9420
                     /gene="cfa"
                     /locus_tag="JN691_002285"
     CDS             8272..9420
                     /gene="cfa"
                     /locus_tag="JN691_002285"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAY7243719.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9460..10101)
                     /gene="ribE"
                     /locus_tag="JN691_002286"
     CDS             complement(9460..10101)
                     /gene="ribE"
                     /locus_tag="JN691_002286"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAY7243720.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10316..11689
                     /gene="mdtK"
                     /locus_tag="JN691_002287"
     CDS             10316..11689
                     /gene="mdtK"
                     /locus_tag="JN691_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAY7243721.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTATRTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11730..12986)
                     /locus_tag="JN691_002288"
     CDS             complement(11730..12986)
                     /locus_tag="JN691_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243722.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13294..13370
                     /locus_tag="JN691_002289"
     tRNA            13294..13370
                     /locus_tag="JN691_002289"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13328..13330,aa:Val,seq:gac)
     gene            13375..13451
                     /locus_tag="JN691_002290"
     tRNA            13375..13451
                     /locus_tag="JN691_002290"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13409..13411,aa:Val,seq:gac)
     gene            13559..13864
                     /locus_tag="JN691_002291"
     CDS             13559..13864
                     /locus_tag="JN691_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAY7243723.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            13990..15525
                     /locus_tag="JN691_002292"
     CDS             13990..15525
                     /locus_tag="JN691_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HAY7243724.1"
                     /translation="MKKIAIVGAGPTGIYTLFSMLANIASIEIPPINCTYLEWLQKQE
                     ASHLQRYGVKKETLHDRQFLPRILLGEYFRDQFLRLVDQARQQKFAVAVYESCQVTDL
                     QITNAGVMLATNQDLPSETFDLAVIATGHVWPDEEEATRTYFPSPWSGLIEAKVDACN
                     VGIMGTSLSGLDAAMAVAVAVAVAVAVAIQHGSFIEDDKQYVVFHRDNASEKLNITLM
                     SRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKGEEGLLDRVFRLIVEEIKFADPDW
                     SQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNLQEVERNKREKHTVPWRYVILRLH
                     EAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPSESIRRLLALREAGIIHILALGED
                     YEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKVKDIPFPGLREQLQKTGDEIPDVG
                     EDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTACAEIGEAMARAVVKPASRARRRL
                     SFD"
     gene            complement(15537..16349)
                     /locus_tag="JN691_002293"
     CDS             complement(15537..16349)
                     /locus_tag="JN691_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243725.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(16353..17138)
                     /locus_tag="JN691_002294"
     CDS             complement(16353..17138)
                     /locus_tag="JN691_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HAY7243726.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(17135..17803)
                     /locus_tag="JN691_002295"
     CDS             complement(17135..17803)
                     /locus_tag="JN691_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7243727.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAYIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(17867..18505)
                     /locus_tag="JN691_002296"
     CDS             complement(17867..18505)
                     /locus_tag="JN691_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HAY7243728.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(18518..20620)
                     /locus_tag="JN691_002297"
     CDS             complement(18518..20620)
                     /locus_tag="JN691_002297"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HAY7243729.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPLGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLIPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGKEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKPMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
BASE COUNT         5134 a         5376 c         4856 g         5318 t
ORIGIN      
        1 gcgcctcatt tcacgctcct gttgaccacc gggtcttaac tggaatttaa atgcctgtaa
       61 tcgtttcaca ggtcaaatga tactttggtc tgtgaaaaaa gaaaccgata tccggcgtgg
      121 aaggcattgt gttttcctga tgcatgttca cctggtcttt gtcaccaaat accgacgcca
      181 gatttttgac catgacacga cagaaaaact acgcacttac ttttcaaatg tatgtgctga
      241 ttttgcagct gaactggttg aaatggatgg cgaaccagat cacgtccatt tgttaattaa
      301 ctatcctccc aaactggcga tatccagtct ggtaaacagc ctcaaaggca tatcgaatag
      361 gttactgcga cgagatcgac cagatattgc agtcaggtat tactacaaag gcgttttgtg
      421 gagtcctggc tattttgcca gtagctgcga aggtgcgcca atatccgtca tccgccaata
      481 cattgaacaa cagcaaacac ctggtcaggt ggaaaaccgc gccttatatc cccgccctga
      541 aggacgaggt tttacggcgc accggataaa ggactgacgg atgcccgttc gcatccgtca
      601 gtattgcagg acggattatt ccgcgtccgg ctcttcagac ttgtatttag cggcagtttc
      661 tttgatcagc tgctgcagtt cgccacgctg atacatttcg atcacgatat cacaaccgcc
      721 gaccagctcg ccgtcaaccc acagttgcgg gaaggtcggc cagttagcat atttcggcag
      781 ttccgcacga atgtccggat tctgcagaat atcaacatag gcaaaacgtt cgccacatgc
      841 ggcaagcgcc tggactgcct gggcagagaa accgcagctc ggcagtttcg gtgaaccttt
      901 catgtacagc aggatcgggt tttcagcaat ctggcgttgg attttttcga tagtggtgct
      961 cattgtcttg cttcctcaaa cttctttacg gcagtaatct gacattgtag cgggtcagtg
     1021 cggcatcgga aaataacatt ttcatcacgc ttttgctatt ttatcccttt gctctatttg
     1081 ttgcatttca aatattcgtt tcttctctga tgcgcggcaa actggcgtat ttataaccat
     1141 ttttagcttt cactgctgca tttttttgcg ctcgccaacg aaacgtattt tttaacaata
     1201 aaagctatta actttctctt cttctatgca ttagaatcat caagttttgt aaatcagacg
     1261 caggcatgat agacctgcct taacagaggg acgctcagtg gcgcggataa accgtatttc
     1321 gatcacgctc tgtgctttac tttttaccac cctgcattta acgcctatgg cccatgcttc
     1381 aaagcaagcc agggagagtt ctgctaccac tcatatcacc aaaaaagcag ataaaaagaa
     1441 aagcacggca accaccaaaa agacccagaa agccagccag aaaacagcga aaaaagccgc
     1501 cagtaaaagt acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc
     1561 ttctaaaaac gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt
     1621 taccgaaaag tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc
     1681 cgcgtcagga acccttgccg acgcgcacaa agcgaaggtg caaaaagcca cgaaagtggc
     1741 aatgaataaa ctgatgcagc aaattggtaa gccatatcgt tggggcggca gctcaccgcg
     1801 taccggtttt gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg
     1861 tattccgcgt acggcgaatg aaatatatca cctgcgtgat gcaggaccta tcgaacgtag
     1921 tgagctgaaa aacggcgacc tggtcttttt ccgtactcag ggacgcggta cagccgatca
     1981 tgtcggcgtg tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat
     2041 tcaaatcact tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt
     2101 aatgacccca aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt
     2161 catcttttgt ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta
     2221 cgtatgcaaa ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat
     2281 atgctaaaga tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca
     2341 agcatcatca gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag
     2401 gtaaatcact ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc
     2461 aggtctggaa ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga
     2521 ctggaaaagt cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc
     2581 agtttactga tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca
     2641 gcgatggcaa actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg
     2701 cgactccgct gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg
     2761 cacgtcctgg ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa
     2821 atctcgctgc ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat
     2881 gccaacgcgg atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa
     2941 ctgtaacgca gaaggttatc cttctgcgtt tttgtttaat tagctgttag caacgcaaac
     3001 tgtttcaggt tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat
     3061 cgcccccgtc accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc
     3121 agctgctccc agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc
     3181 tggcgcttca ctggcgacat gcatcacgcg catctgtaac ggaggtacaa ccgcaaaggt
     3241 tgctgcgccc cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg
     3301 gattgccagc ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga
     3361 acgatctgcc agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac
     3421 cagcattgcc gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat
     3481 ataggtgtag agggtaaaca ttgcaccagc tcccagtacc gtcgtcagca atgcagacag
     3541 cacctgcgga cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc
     3601 acctttaggt aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc
     3661 cagaaatgac atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc
     3721 gatatttgcc agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt
     3781 tggcaccacg cttgcggcca cgactgaacc caaacccaaa aatgctccgt gattcaggct
     3841 ggtcaaaata cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagaacgtt
     3901 gcctggcgtg aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag
     3961 tagaagcgtc atcagcggcg agcccaccat tacgccaact gcataggcac tgattaacat
     4021 tccggcagcg ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg
     4081 cgagaactcc gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt
     4141 aattttcatg ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga
     4201 aagcatgaca gcaatcacaa aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt
     4261 ttgctaacaa acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg
     4321 cagcgcggca gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag
     4381 tgaaaattta aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg
     4441 atattacgca tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg
     4501 tttttaccac ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg
     4561 tactggccat tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt
     4621 ttgttgtgcg taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag
     4681 ttagggtctg gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt
     4741 ttccactaca actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg
     4801 caacgccgtg tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag
     4861 cctgaaggtt aaccacacca agtctatcgg tttgttggcg accagcagcg aagcggccta
     4921 ttttgccgag atcattgaag cagttgaaaa aaattgcttc cagaaaggtt acaccctgat
     4981 tctgggtaat gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca
     5041 aaaacgcgtc gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat
     5101 gctggaagag tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga
     5161 cttcaccgat gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct
     5221 gattgaacgc ggtcaccgcg aaatcggcgt catccccggc ccgctggaac gtaacaccgg
     5281 cgcaggccgc cttgccggtt ttatgaaggc gatggaagaa gcgatgatca aggtgccgga
     5341 aagctggatt gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat
     5401 cctgtcgcag ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg
     5461 cgcactttgt gctgctgatg aaatgggcct gcgcgtcccg caggatgttt cgctgatcgg
     5521 ttatgataac gtgcgcaacg cgcgctattt tacgccggcg ctgaccacga tccatcagcc
     5581 aaaagattcg ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg
     5641 tgaagaaccg cagtccattg aagtgcatcc gcgtttgatt gaacgccgct ccgtggctga
     5701 cggcccgttc cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg
     5761 cagccactcc ttattcagcg tctcactatc gccgagatac tcaagcagcc aggttaacgc
     5821 aggcgacata tcattttgct gccatgtcag acaacatgcc gaatccggga aggggttttc
     5881 cagttcaagt gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac
     5941 catccctatg cataatcctg ccgagataca ggttgccgat gattcccagt caggtacgac
     6001 aagtcttttt tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc
     6061 ttcgcgcacc aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa
     6121 cgccagcggg tggtgactgg caacaacgca actccagctt agcatcccca tatcccggaa
     6181 ggcataacga ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc
     6241 gtcggaaagc gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc
     6301 atcaaaatgg cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt
     6361 atccactgcg atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg
     6421 ggtgatctgc atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc
     6481 ggtcagctcc acatcacggt gccgtcgttc aaagagcggt accgccagcc actcttccag
     6541 ctgacgcacg gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct
     6601 aaaactacca ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt
     6661 ctgcctgcaa aatttttgaa accagtcatc aaatattacc gtttcacaac actaatttca
     6721 ctccctacac tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg
     6781 gaaaagattt ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat
     6841 gtatctgcct gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag
     6901 tgccagcctt agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccact
     6961 ctccgaccgt tatggtcgta aaccggtgtt attaatcggc ctgacaattt ttgcgttagg
     7021 tagtctgggg atgctgtggg tagaaaacgc cgccacgctg ctggtattgc gttttgtaca
     7081 ggctgtgggg gtctgcgccg cggcggttat ctggcaagca ttagtgacag attattatcc
     7141 ttcacagaaa gtcaaccgta tttttgcgac catcatgccg ctggtgggtc tgtctccggc
     7201 attggctccc ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc
     7261 caccctgttt gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac
     7321 gaaggcccgt aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac
     7381 ctatcgcggc aacgtgctga tatacgcagc ctgttcagcc agtttttttg catggctgac
     7441 cggttcaccg ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta
     7501 tgtcccgcaa actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cactgcagaa
     7561 atggcaaggc aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc
     7621 gacctgggct gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg
     7681 tgtgatggcg attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc
     7741 cttcccacat gcaaccggtc gcgccgcagc gttgcagaac actctgcaac tgggtctgtg
     7801 cttcctcgca agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac
     7861 caccagcgtg atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg
     7921 tgaagaagtt gactgcccga atcatggcaa tgccgaagtc gctcatagcg aatcacactg
     7981 atctatatcg atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt
     8041 agcggcctat actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga
     8101 caggatcgtt cccgactcac tatggatggt catttcggca agggttcctc ctttccctct
     8161 gttctacgtc ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa
     8221 agcgtctacg ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca
     8281 tcgtgtatag aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta
     8341 cttagccgtg ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac
     8401 cccgattttt ttaaacgcgt tctgcaggaa ggctctttgg ggttaggcga aagttatatg
     8461 gatggctggt gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt
     8521 ctcgagaacc aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc
     8581 ttcaatctgc agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt
     8641 aatgacttgt tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa
     8701 gatgccgata atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg
     8761 cagttaaaac cagggatgcg cgtactggat attggctgcg gctggggcgg actggcgcac
     8821 tacatggcat ctaactatga cgtaagcgtg gtcggcgtca ccatttctgc cgaacagcaa
     8881 aaaatggctc aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt
     8941 gacctgaacg accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg
     9001 aaaaattacg atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggtatattc
     9061 ctgctccata ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa
     9121 tatatttttc cgaacggttg cctgccctct gtacgccaga ttgctcaatc cagcgaaccc
     9181 cactttgtga tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg
     9241 tggtatgaac gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt
     9301 aaacggatgt ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt
     9361 cagctctggc aggtggtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa
     9421 aggctattct atcgccccct cttcgggggc gagttcagat caggcttctg tacctggttg
     9481 attcatggca ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt
     9541 ttgtggatcg atttcaatgt tgacgcgtgc gccaagtttt ttcttcccaa gagtcgtgcg
     9601 ttccagtgtt tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt
     9661 caggctaata ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact
     9721 atcctggact ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc
     9781 agcggtggtc ataatatgac ctgacattaa gtgtccgcca atttcatcac tgaatttcgc
     9841 cgcacgctca acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa
     9901 cgtttctttc atcaggtcaa aactgacatg gttgccgtta atttccgtca cggtcaggca
     9961 gcaaccgtta tgcgccacgg aagcaccggt ttccaggccg tccagcatgt ggtcgggtaa
    10021 ctccaccaca tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc
    10081 ctgtacaatc cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag
    10141 cacaatagca ggtggaaaac gcccttacca gtgaaggggt aagaatggct attttttcac
    10201 tggagaatta ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct
    10261 gcccattcag gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca
    10321 gaagtatatc agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca
    10381 aatcgcccaa actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac
    10441 cgacatggcg gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca
    10501 cggactgctg ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga
    10561 gcgcattgcg catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat
    10621 tatgctggtg ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc
    10681 tctggcggac aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc cgggatatct
    10741 gttcttccag gttgcccgta accaatgtga aggtctggca aaaaccaagc cgggtatggt
    10801 aatgggcttt atcggcctgc tggtgaacat cccggtgaac tatatcttta tttatggtca
    10861 tttcggtatg cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg
    10921 ggtcatgttc cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatattcg
    10981 taacgaaaaa ggcaccgcta agcccgatcc tgcggttatg aaacgactga ttcaactcgg
    11041 tttgccgatt gcgctggcac tgttctttga agtgacactg tttgccgtcg tggctctgtt
    11101 agtgtctccg ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc
    11161 actgatgttt gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg
    11221 tctgggtcag ggctcaacgc tggatgcgca aaccgctacg cggaccgggc ttatggtggg
    11281 tgtctgtatg gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct
    11341 gttgtacaac gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc
    11401 ggtatatcag atttctgact caatccaggt gattggcagt gggattttgc gtggttataa
    11461 agatacgcgt tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag
    11521 cggctatatt ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg
    11581 gataggcttt attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt
    11641 cctgcaacgt atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg
    11701 cgcaaccttc acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg
    11761 tcttcgtctg gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc
    11821 tcaacgcgta tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct
    11881 ttcgcactgg tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc
    11941 acatgcgttg ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca
    12001 acaatgaaag tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc
    12061 tgctgtacag taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg
    12121 ccgtccagta atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca
    12181 aaattttcgt ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca
    12241 ttttccagcg taccggcatg cacttcagcc cgacccccca cattcaccga caggttttgc
    12301 gtcttaccgg aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc
    12361 tcaacaatag tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac
    12421 gtatcactgg ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg
    12481 gcttctccac ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt
    12541 gtcccattaa ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct
    12601 tccagtacac gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca
    12661 acgctgaatt caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt
    12721 gcgcctccct cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg
    12781 ccattgttac cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca
    12841 gcccccgtga ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca
    12901 gtaagttgcg tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc
    12961 atcaagtttt tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat
    13021 tgctacctaa gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa
    13081 acaactctgt ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc
    13141 ctgaataaaa atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc
    13201 aaacggtcga aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg
    13261 ctaatattcg tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca
    13321 ccaccttgac atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc
    13381 gtagctcagt tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact
    13441 cggacgcacc agattttctt aatctggtct tttccttttt ccctctgttt cttctctgta
    13501 tccaatacgt taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat
    13561 ggcaactttg ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga
    13621 gcagcttaaa ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg
    13681 gacagaaagt gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag
    13741 cgcccttgcc tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt
    13801 tgtcgccaaa gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc
    13861 ctgacagaat ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa
    13921 attctaaaaa tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg
    13981 taagtaacaa tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta
    14041 ttctcgatgc tggcaaatat tgccagtatt gaaataccgc cgattaattg tacgtatctc
    14101 gaatggctac aaaagcaaga agccagccat ctccagcgtt atggcgttaa aaaagaaacc
    14161 ttgcacgatc gtcagttttt accgcgaatt ctgctgggcg aatatttccg cgatcaattt
    14221 ttacggttag tagaccaggc acgacagcaa aaatttgcag tggctgttta tgaatcatgc
    14281 caggttaccg atctgcaaat tacaaatgct ggcgtcatgc tcgctacaaa tcaggattta
    14341 cccagcgaga cgtttgattt agcggtgatt gccacgggtc acgtctggcc tgatgaagaa
    14401 gaagcaacca gaacgtattt tcccagcccg tggtcaggcc tgatagaagc aaaggtcgat
    14461 gcgtgtaacg tgggtattat gggaacatcc ttgagcggac tggatgcggc aatggcagtg
    14521 gcagtggcag tggcagtggc agtggcagtg gctattcagc atggttcgtt cattgaagat
    14581 gataaacaat acgtcgtttt tcaccgcgat aacgcaagtg aaaagctaaa tattacgttg
    14641 atgtcgcgca cggggatttt acccgaagcc gatttctatt gccctattcc ctacgagccc
    14701 ttacacatcg tcaccgatca ggcattaaat gctgagattc aaaaaggtga agagggcctt
    14761 ttggatcggg tatttagatt gatagtagag gaaatcaagt ttgctgatcc agactggagc
    14821 caacgcatag ccttagagag cctgaatgtc gattcctttg ctcaagcctg gtttgccgag
    14881 cgcaaacaac gcgacccatt tgactgggca gaaaaaaatc tccaggaagt cgaacgcaat
    14941 aaacgagaaa aacatactgt tccctggcgt tatgtcattc tgcgcctgca tgaagccgta
    15001 caggaaattg ttcctcatct gaatgaacac gaccataaac ggttcagtaa aggccttgcc
    15061 agggttttca tcgataatta tgcggcaatc ccttcagagt ctattcgtcg actactggcc
    15121 ttacgtgaag cggggatcat tcatattctc gccctcggtg aagactacga aatggaaatt
    15181 aatgagtcgc gcaccgtcct gaaaacggaa gacaacagct actcgtttga cgtttttatt
    15241 gatgcccgcg gacagcgtcc gcttaaagtg aaagatattc ctttccctgg gctacgcgag
    15301 caattacaga aaacagggga tgaaatccct gatgttggcg aagattatac gttacagcaa
    15361 cccgaagata ttcgtggacg cgtagcgttc ggcgcgttgc cctggttgat gcacgaccag
    15421 cctttcgttc agggacttac ggcatgtgca gaaattggtg aggcgatggc tcgggcggtc
    15481 gtaaagcctg catcccgtgc tcgtcggcgt ctttcgtttg attaaaggta aagctatcag
    15541 cactcaacaa actgatgctg ttcattaaga cgatagcgga ctccggttcg aatattgttt
    15601 tcaccttcaa tggcgacggc gaagcgaact ctttcgccgt catgatatgc cagtgcggcg
    15661 cttccccctg gcccgagaat aattgagtcg accacgcccg tgctcactac cgttgaattt
    15721 tcaccgctag caatgatgtg tacattgtcg ccgctgttgg caattctggc gtttgcgcca
    15781 aagctggcga tttgcaccag gtcaccatta ctggcgacat gacaccgttc acccaatgta
    15841 caaacgcgta ctcgcattcc ggtattcgca atccgactac tatctccagc gctgcttatc
    15901 cgcgaagaat taccggttac agcaacccgt gcgcgttcac cggagctgcc aatgtgactg
    15961 ttaaagccga cacttccaat ccgcacgcta taacccatac tggcaatttg cgcggcatac
    16021 ccagcggttg cgattcgggc gttatcctca caacagccga tgcgagcatg atcgtcgctg
    16081 cgggcaaact gactgcaaaa tggtatctct ccggggtctg tggccagttt ttgcatagag
    16141 gaaacttcct gatgcgcgaa gttttcatcc gccagccatt ttttccacgc atactcaacc
    16201 agactctccg cccagtccgt atatccttcc tcaatcagtg catgacgaat atcggcataa
    16261 ctaccgccgc gtgggaaatg gcgcaggaac cagcgataca tcaccgctcc gatacgccat
    16321 tctcgcaggt ctgctcgtgt aatgatcatt cgttagtgcc ggtgatagcc atcgaccatg
    16381 cttttaaagg tttcgtgtgg cgtacgtccc gtggtgcaaa gataaagatg accgaagata
    16441 aaaaagaggc ttataaatgc cagagcaaaa tgcgcctgca ataaccagta tcttacgcca
    16501 ggaaacacat ctcccacggc ttgcggatag agacacaaca gccccgtcaa tagtaacaac
    16561 ggcagcaatc catacatgac accaacatag gcgacctgct gtaagggatt aaatttagac
    16621 tgtgttgttg ccgggaaagg atgttcttcc ccctgcataa tgccaaacaa ataaaatcgc
    16681 gtttgttttg ccgctcgttc cagccacccc tgacgacgaa tgcgatagtg gtgaccatta
    16741 tccccaacgg cattgatcag cacaaagccg agccagcatg ccagtaacaa aaatccgcaa
    16801 acttcatgca ccgcaaccag acttttaacc gcagttgcgc ccaccatcgc aaagtgattt
    16861 atcagcccac ttgccagcaa caatacaaag agtaacgcat tcgaccagtg ccataatctg
    16921 accgctttgc tgtaaagata aaccttctca ccatgatcgg tggctgattt cttcacgcca
    16981 cgagcacgaa gcaaggcgtg cagccccagc accaaccaca tcccaaccag caatactccg
    17041 gcaatgatca accacaccgg ccaaaattcg ggagtgaact gtggcacata gttcgctaac
    17101 tggctctgaa actgttcagc atgttgcgac gggttcatac attttccttt ttaatcaaat
    17161 gttgaccgaa ccggcgatac agatgcggtt ttccggcacc aggtagctga tattgatagt
    17221 atttattatc ctgcaaccac gcctgaattt ctgggctatc ttcgcgacca aagatcaatg
    17281 cgtgttccgg acaggcgctg acgcaaatgg gcggaaagcc ttttgccagt cgggactcag
    17341 cgcagaaatc acatttatct gccactttag tgacagggtt aaggtagcga acctggtacg
    17401 gacaagcgcc aatgcaataa ctgcagccga tgcactgcga tttttctacc cgcacgatcc
    17461 cctgctcatc acgccacgat gcccccgtcg ggcatacgtc gatgcacgga gcatcttcgc
    17521 agtgctgaca tgactggcga aaaaagtgat actgcgtttc gttgtcgtta tcggtgactg
    17581 gaatgtatgc tatcgataaa cggcttccct gagcggggac gtggttagtt ttacggcatg
    17641 cgcgggcgca aatattacag ccattgcacc gtgactcgtc atgaatcatg gcataccgaa
    17701 cttccttttc ttgcctcgtg ttcgctaaca gagaagacgc agaaccagta aaaaagatta
    17761 ctgttcccat ccccagaaca aatttgcgtc gagtgaagga catctgatta tcccctaatg
    17821 cagtggctgc aactgtccac cctatccatg acatctccct gaaatttcag gggttctgaa
    17881 actgctctac ctcgaaccat tcgcataagc cataagcctt gccataggtc ataaatcgct
    17941 cgctaaacaa gtacaacacc gccgtcgatg actccacctg ccgtatatcg gcatattcag
    18001 gtgcaacatc catcgcggcg atcgcggctt caatctgcgc ttcctggaag taatatggcg
    18061 cttgcatcag catggcgact ttgtaaggac gaggatacgt ctggcactca aaacgaacgg
    18121 cgtgtgcaat ggcgcgacaa atgtcctgct ccacaacttg caaagacatc gccgcgtagt
    18181 tttcactcat ggcctgcgtt gagtaatagt aatcatcctg acttcccgtc atacaagcga
    18241 tatccgcgta gtctccgttc tgcttaatgc tttccagcaa aactgacagt tcctcttctg
    18301 caaccgaata gggtggcgag agaaacacgg tgcgcgccac cagctgccca gccgcagaat
    18361 gctggcgaat gaactcagcc aggatttcag caggttctgg ttcggttacc gtatcaacgg
    18421 ggtgacgcat cccctgcaac cattgccgct tcgcctcgtc aacttcgctg actttcgcta
    18481 acggcaattc atcctgatgg ttcatcttac ccattcctta cgcaggcagt agattgtgtg
    18541 ctgccagatc ggcggcaata tcttccagcc ccagacgctg caatgtttcg cgggttggac
    18601 aaccaagctg tgggtcccag cccatttctt tgtagaacat cgtgagcgaa gcatgcatat
    18661 cgtcacgatc cattttgtca gtaccttcag taaagaccgg gatctgcgga tccttgtcga
    18721 aaacccagga acagataaga tcgtgttcat tacgcatatc tttggtttgc atcggtttta
    18781 ccgtgtaggc acgatgcaac gtaaagatac gctctgcagc taaatctaat ttttcctgag
    18841 tcatctcttc gccggtgatc gctttgaaga atttggcttc aagcgccaga tcgccccggt
    18901 aattacggct tttcagcggc gaaacggtca ttggccagac ccagttgcac agggtgacgg
    18961 cgttatgcaa acagacccgc aacagcgacc actttgcata tttaattttt gcgtcattga
    19021 ttggcgtgta gtttttggtt tcatcgtaag catcttcaga accaaaaagt tctttcgcca
    19081 cttcacgttg cagtttcaat ggcaagccgg aaccaataaa gttgatatgg gtatgcgtca
    19141 tgcagtcacg gttgaacata cagttaacaa tggaaccgac ctgcgctgac gcttcattgg
    19201 catggtgaac cggatagcca aacggcgacc agagtttatt tttcgcatag ccccagtact
    19261 ctttacccaa attccagcgt tctgcgatgg catatgaacc atcagccagg tgactcagct
    19321 cacccacacg atgcgccaga cggtagtaaa aatctttaat gaagttaacg tcacccgctt
    19381 ccagttgatc ccagcgaatt tctgcatact cttcagctgg cagaacacgc ttgaacacac
    19441 ctttgctgta acaataagta aagtcgcgat gcaactgccc gtagttacac cataggccgt
    19501 agtcatcgaa caaattcaga ccaaccaggt taccaatcac acgaccatcg tctttatctt
    19561 caaaatcttt cggcccgttc gggaagatgg tggtatggac aaagtttgct acacaagtgt
    19621 taccacctgt gctgggcacg ccaaactctt tcacccgagg aatattcatt tgggtcatac
    19681 aacggatcgg gcaagagtgg cagccgctca tttttactgt gtatttctct gccgccggtc
    19741 ctaagtcaaa aacggattta taggtacgaa agccgaccgt attctgattg cctggcggaa
    19801 tttcacccgt ttcaatcgga ccaccttcag ccgcgcccca aaacagccct ttacgtgctg
    19861 tccagcgtga cttgggatct gaatactctg cccacgattg tggcgtactt ggcacgacat
    19921 ggttgttatt cgcaccaata agttcagtca tcatgtaatc attgagacgc ttcatctcct
    19981 gacgatcggc aatgttgacc cctttcgtcc cttcaaccgc aatcgctttc aggtttttcg
    20041 aacccattat tgcgccagtt cccgcaccgc cgctgtggtt acggctattc aacatgccag
    20101 agagaggaat aaggttttcc ccagcctgac caatagccgc cacacaggtt tccggactgg
    20161 tcaatcgaca aatttcttcc gtcgttgcgc gcgtcccttt tccccataag aaatcggctt
    20221 tttccaggct aactttgtca tctttaatct tcagccatac cggtgatttc gccttccctt
    20281 cgataataat gacgtcgtat ccagcgaatt tcatttgcgc tgcaaaaaag ccacccatat
    20341 gggcatcgac gactaaattt cctttggtaa aagtagaaag tgaggtgata tttacgcgag
    20401 aactacaggg ggcaccagat ccagttaatg ggccggtagc aaagactaat ttattcgctt
    20461 catcgaaagg tttcgtgcct agcggtactt cgtcatacat aattttgtag ccgaagccca
    20521 tgccaccgac aaaactttta aacttactgg aatcttcgag ggtaatattt cctgtcgtga
    20581 gattgactct taatatatta cctgtccaac cgttagccat gatttttttc tttgcaagat
    20641 tcacacagta atatctttcc actcgataat ttttaacgcc cctg
//
LOCUS       DADLSA010000063        20621 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.063, whole genome shotgun sequence.
ACCESSION   DADLSA010000063 DADLSA010000000
VERSION     DADLSA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.063"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..848
                     /locus_tag="JN691_002298"
     CDS             <1..848
                     /locus_tag="JN691_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7243730.1"
                     /translation="VLVVFVGILLLMAFVVLPIAWQQGIYLIRDMPGMLNKLSDFAAT
                     LPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLLTIAVYLVLVPLMVF
                     FLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVLEMIVVGIATWLGFL
                     LFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGTEFWSCFAVYLIIQA
                     LDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPLATLIKAVIHAWPDG
                     QIAQE"
     gene            complement(886..1734)
                     /locus_tag="JN691_002299"
     CDS             complement(886..1734)
                     /locus_tag="JN691_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HAY7243731.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAILHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(1731..3767)
                     /locus_tag="JN691_002300"
     CDS             complement(1731..3767)
                     /locus_tag="JN691_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAY7243732.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEELLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCTAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVRRCCNLLLQEYA"
     gene            complement(3797..4210)
                     /gene="hyfJ"
                     /locus_tag="JN691_002301"
                     /pseudo
     CDS             complement(3797..4210)
                     /gene="hyfJ"
                     /locus_tag="JN691_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
     gene            complement(4203..4961)
                     /gene="hyfI"
                     /locus_tag="JN691_002302"
     CDS             complement(4203..4961)
                     /gene="hyfI"
                     /locus_tag="JN691_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HAY7243733.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(4958..5509)
                     /gene="hyfH"
                     /locus_tag="JN691_002303"
     CDS             complement(4958..5509)
                     /gene="hyfH"
                     /locus_tag="JN691_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HAY7243734.1"
                     /translation="MLKLLKTIMRAGTTTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTV
                     THKADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCP
                     ECKQRATLINDDTDVPLVAKEQL"
     gene            complement(5519..7233)
                     /gene="hyfG"
                     /locus_tag="JN691_002304"
                     /pseudo
     CDS             complement(5519..7233)
                     /gene="hyfG"
                     /locus_tag="JN691_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            complement(7223..8803)
                     /gene="hyfF"
                     /locus_tag="JN691_002305"
     CDS             complement(7223..8803)
                     /gene="hyfF"
                     /locus_tag="JN691_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HAY7243735.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     ARGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGILKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(8808..9458)
                     /gene="hyfE"
                     /locus_tag="JN691_002306"
     CDS             complement(8808..9458)
                     /gene="hyfE"
                     /locus_tag="JN691_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HAY7243736.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(9470..10909)
                     /locus_tag="JN691_002307"
     CDS             complement(9470..10909)
                     /locus_tag="JN691_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
                     /protein_id="HAY7243737.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLLGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(10926..11873)
                     /gene="hyfC"
                     /locus_tag="JN691_002308"
     CDS             complement(10926..11873)
                     /gene="hyfC"
                     /locus_tag="JN691_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HAY7243738.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(11884..13901)
                     /gene="hyfB"
                     /locus_tag="JN691_002309"
                     /pseudo
     CDS             complement(11884..13901)
                     /gene="hyfB"
                     /locus_tag="JN691_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(13901..14518)
                     /gene="hyfA"
                     /locus_tag="JN691_002310"
     CDS             complement(13901..14518)
                     /gene="hyfA"
                     /locus_tag="JN691_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HAY7243739.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(14771..15241)
                     /gene="bcp"
                     /locus_tag="JN691_002311"
     CDS             complement(14771..15241)
                     /gene="bcp"
                     /locus_tag="JN691_002311"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAY7243740.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(15241..15813)
                     /locus_tag="JN691_002312"
     CDS             complement(15241..15813)
                     /locus_tag="JN691_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAY7243741.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKSTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15959..16837
                     /gene="dapA"
                     /locus_tag="JN691_002313"
     CDS             15959..16837
                     /gene="dapA"
                     /locus_tag="JN691_002313"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAY7243742.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAACDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16854..17888
                     /gene="bamC"
                     /locus_tag="JN691_002314"
     CDS             16854..17888
                     /gene="bamC"
                     /locus_tag="JN691_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAY7243743.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            18101..18814
                     /gene="purC"
                     /locus_tag="JN691_002315"
     CDS             18101..18814
                     /gene="purC"
                     /locus_tag="JN691_002315"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAY7243744.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18982..19845
                     /locus_tag="JN691_002316"
     CDS             18982..19845
                     /locus_tag="JN691_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAY7243745.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNHLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19860..>20621
                     /locus_tag="JN691_002317"
     CDS             19860..>20621
                     /locus_tag="JN691_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY7243746.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDE"
BASE COUNT         5185 a         5474 c         5546 g         4416 t
ORIGIN      
        1 ttgtattggt ggtttttgtc ggtatattgc tactgatggc gttcgtggta ctgcctatcg
       61 cctggcaaca gggcatctac ttaatccgcg atatgccggg gatgctcaat aagctttctg
      121 actttgccgc cacgttgccg cgccgctatc cggcgttaat ggacgcgggc attattgatg
      181 caatggccga aaatatgcgc agtcggatgc tgaccatggg cgattcggtg gtgaaaattt
      241 ccctcgcctc gctggtcggt ttgctgacca tagccgtcta tctggtgctg gtgccattga
      301 tggtcttctt cctgctgaaa gacaaagagc agatgctgaa cgccgttcgc cgggtgctgc
      361 cgcgtaaccg tgggctggca ggacaggtgt ggaaggagat gaatcaacaa atcaccaact
      421 atatccgcgg caaagtgctg gagatgatcg tggtggggat cgccacctgg ctggggttct
      481 tgctcttcgg gctgaactat tcgctgctgc tggcagtgct ggtcggcttc tcggttctta
      541 ttccgtacat tggcgcattt gtggtgacca ttccggtggt tggcgtggcg ctattccagt
      601 ttggcgcagg tactgaattc tggagctgct tcgcggtgta tctgattatt caggcgctgg
      661 acggcaacct gctagtaccg gtgttgttct ccgaagcggt taacctgcat ccgctggtga
      721 ttattttatc ggtggtgatc ttcggtggtt tgtggggatt ctggggcgta ttcttcgcca
      781 ttccattggc gacgctgatc aaagccgtga tccacgcctg gcccgatggg caaatcgcgc
      841 aagaataatc ttcagcccga cgaactgttt cgccgggctg atttttcagg attcctgacg
      901 taaataaata gcccgataac aaatactcac cagcactgca ccgccgataa tattacccag
      961 catcaccggg agcagattgg cggtaataaa atggctgacc gtcagtgccg gaaaattgtc
     1021 tgcgctactg tgcgccagtt gccagaagga ggtaggggcg aaatggcgaa tggcaatggc
     1081 gaacggaatc acaaacaaat tggcgataca gtgctcaaag ccactggcga caaacagggt
     1141 gatgggcaaa atcatggcga cgattttgtc gcataacgaa cgtccgcaat aactcatcca
     1201 cagcgccagg caaaccatca gattgcacat aatgccgagg ctgacagatt cagtaaatgt
     1261 atgatgcatt ttgccctcgg cgcagtgtaa aatcgcgact ccccacatcg cgttttcact
     1321 catcaccagc ccggaaaacc agattaacaa actgaaacag gcaatacctg ccagattacc
     1381 gcaggccacc agaagtgcgt taatcagcca agttcgccaa ctaataacgc cccgactttt
     1441 tgccattacc gtcattaccg acgaggtgaa caggctggtg ccgcaaaccg ccagcaaaat
     1501 aaagccaagt gtaaagcaaa ggccgcccac cagatgggtt aatgcctgtg aagacggggc
     1561 atcggcgata acggaaaggt aaaaagtaaa tccgatcgcc ataaacacgc cagcggacat
     1621 cgccattaaa aagaccgaca ctttactgcg ggcaatttta tgggcggcaa tccgttcagc
     1681 gattgccgct tttctggcgc tcaactgcaa gtcgaaagag agtttgtttc tcatgcatac
     1741 tcctgcaaaa gcagattaca acacctgcga accgagattc ccagacgctg cattcgtgac
     1801 agcagcgtgg tgcgcttcat ccctaatcgt gtcgccgcgc cacggggccc ggcaacaatg
     1861 ccattggttt ctcgcaatac ctgaacaatg cgctgacgtt cttcttcgtc attctctggc
     1921 gtcgtcgggt gcagcaactg cgccattgaa ctgcgaagcg ctgaatcttc atttagcgtc
     1981 ggcagtaaac ggctttgtcg gacattaaga tgtaaattca gactgttacc gcgagtcagc
     2041 agtaccgctc gctcaatcac gttttccagc tcgcgcacgt tgcccggcca gtcccacgac
     2101 atcaactggc gtagcgcctc ggtcgggatg gcgtcaattg aacgattcat atggcgcgcc
     2161 attttttgcg tgaaatgctt tgctaaaaga gggatatctt ccggacggtc gcgcagcgga
     2221 ggcaattcca gtgggaagac attcaggcga taaaagagat cgctgcgaaa ctggcgatct
     2281 tcaaccattt gccacaaatc acggttggtg gcggcaatga ctctgacatt taccgggatc
     2341 gttctactcc cgccgagacg ctcaatctcc cgttcctgca atacgcgcag cagtttaggc
     2401 tgaagttcta acggcagatc gccaatttca tcgagaaaca acgtgccgcc atcggcaatt
     2461 tcaaaacgac cacgatgggt attaatcgca ccagtaaacg cccctttgtc atgaccgaat
     2521 aactcacttt ccagaagact ggcggggatg gcagtgcagt tgattttgac cagcggcttg
     2581 tcgtgtcgcg ggctaagttg atggatcgct ctggcgatca cctctttgcc ggttccggtt
     2641 tcaccgcaaa tcaacaccgt actgtcgctc ttcgccacaa tatctacctg ctggagcagc
     2701 tcttccattg cctggctttg atagataatg tcaccgacgc ccagattcga aaggagctgc
     2761 tcgctaagct ggtggttttc ttgctgcaaa ctttcctgca aatcggtcat tctacgccag
     2821 gcatcggcat tgccaacggc aatagcgatg cgatcggcta tgtgttgtag tagctggcag
     2881 ttttcctcac taaagagagt ggaagaggta tgcgccagca acaatgcacc cggtgtatgg
     2941 ttgccaaagg taagcggtat aaggagcgca gattcgcagc cgttagatgc aagtaagagt
     3001 aataacgggt cgcgttgcca gagaaacaga tcatctgcac ggtgcgtcaa ggtcggttgt
     3061 cgggtttgta gcgtttgtgt cagcaataca ctgtcgccag gcatattgcg tggcagacac
     3121 gcacaatgtg aggcagaaag atcgctgcac cacaggctga acttctcgtt ctttcgatga
     3181 tcgcccagta ccatactgac tgaagccaga ccgaaaaaat gatggatctc acgagcgacg
     3241 tcagcgatca gatcgtcgag atcaagatgt gacagcactg cattagtgat atccaccagt
     3301 acccgatgat gatcgcgatc tttctccagc aaacgcagcg ccacctgctg ttcttcatca
     3361 ttgagccatg attgcagaac cggggtgata atgcgagcca gccagttaaa cagtgtgcaa
     3421 tcctggggag taaaacggct gccgtgggta cgaataaagc gtagctccgc cattccacta
     3481 ctgccgagaa ggacggcgtg acgcgaataa tcgaccatct cgccctgata ctctgttgtt
     3541 gccgtcacat cgctgccatc atcatggtag cggtaataga agttgctttt gagaacatgc
     3601 agttcaataa gttgtaccgg gctgaacggc ggcggcagcg ggatgaaggc gcgtaataaa
     3661 gacgccttgc cgtgtttctg cgctaaccgc tccgcgagca ttccgtttac cgcttcaatt
     3721 gttattcctt gcggcggggc aaacatcgct tcgtctgaca tagccatcaa cagtccttat
     3781 taatgagaaa tccttctcac gcaatttttc tggccatcag atagatagcc ggttccagca
     3841 caatttcatc caacagatga atgaattgaa tgctacgcgc ttgtcacaca gacgtcgcgt
     3901 catctgccag tggcgcgaat gcccgggtca acagggcggt gtggctggcg tcaataacaa
     3961 tctcaccaaa agtcatcacc cccagcatct tacgtcgggc ttgctcctct tcgaccagtg
     4021 caagccaatc ttcgtattcc gtcagtgggc agcggaaggc gacattcaga caatcaatca
     4081 cgccaacgtg atgaccgata gccagcgagt aatacattac ctgagagctg tgttccggca
     4141 tctcgtcgct actggcgaca aacttttttc gcagcgtcca gaaaacaatt tccccgcact
     4201 cttcagtcat gtaacccccg gagtacgcga aagagttgct gaacgatact attgagacgt
     4261 ggatcgtcgg catcgcgcaa ccaggtgtta acccgatttc ctgtaggatc gtcgcttaaa
     4321 tggcgcagga gccgatcgca aatttctcgc ccctgacgat agcccgccag ccgccgcgct
     4381 tctcgctcaa tggcgatacg ctgtgatggc gggatctgcg gccacaacgg ttgcatagtc
     4441 accccggtgg gatcgcgata atccaccgcg tgaatcttct gttgcagcaa accgagcgcc
     4501 acggcgaaac cgtgaatggt ggcggccggt gttggcgggc agccggggat ccaaacatca
     4561 atggggacaa tggtgtcgct gccgccccag acgctgtaga gatcgtggaa aataccgccg
     4621 ccgacaccgc acgcgccgta ggaaacacag attttatgat cgggggcaga ctcatacgcc
     4681 cgaagtgcag gcatacgcat cgcccgggtg actgcgccag taaataacaa aatatcggcg
     4741 tgacgcggtg atgaaacaac cttaatgcca aaacgttctg cgtcgaatac gggggtgatg
     4801 gcagcaaaaa tttcgatttc acaggcgttg cagccgccgc aatcgatgcg ataaacgtaa
     4861 gccgagcgac ggatatcctg tagcaaatgc cgcttcatct tttgcgtttg ctcgtccagc
     4921 gtgatgggct ggctgacata ttgcgtaagc actggactca tagctgctcc ttagccacca
     4981 gcggtacatc tgtatcgtcg ttgatcagcg tcgcgcgttg tttgcactcc gggcagacgc
     5041 tcgcttgcgc ccataacatt tcgcggtttt gtggcgcatt ttgttgctgt gccaacaatt
     5101 cagcagccag tgcgacggtt ttttgcgggg caaacgggcg ttcgcaacgg ctgcaacgtt
     5161 gtagatggaa cgtcgcgtgg gtatagagat cggctttatg ggtgacggtc agttcaaagt
     5221 tattggtaag ctggatggct ctggtcgggc acacttcttc acaacgtccg cagtaaatac
     5281 aacgccccag atagagctgc caggtgcgcg aattttgctg gtcgtcggtc tggatagtca
     5341 gcgcatttgc cggacaagca caggcacagg cgcaggcacc gcaggcaata cattggctgg
     5401 gcatcaggtc cggtttcccg cgaaagcctg ggctgacctc cagtggcgcg aagggatatt
     5461 tcaccgtcgt ggttccggcg cgcataatag ttttcagtaa cttcagcatc ttctgtcctt
     5521 acttcagcgg cgaacggtta cgatcaatgc cgtagcgttc gatctcttta tacggcacgg
     5581 tttttgactg gcgcttgcgc acatctacca gcgtcacacg gtcggtacag gagtagcagg
     5641 gatcaaggct accgataatc agcggtgcgt cagaaacggt attgccgcgc agcatgtaac
     5701 gcaacaccgg ccagttggcg taggtggcgg cacgacagcg ccagcggaac aatttttggt
     5761 tatcaccgag catgctccag tgcacgtctt caccgcgtgg cgcttcaaca aagcccagcg
     5821 cgaatgcgtg aggtttatag ctaaagcctt cggtcagcag tggggtatcc ggcatgttgt
     5881 cgagggcaaa ttccagcatt gccagcgaat caaacgtctc tttgacacgg accatcacgc
     5941 gggagaaaac atcgccgccg gtaaaggtaa acagtgtttt tggaatattg ccgtagtcgg
     6001 cgtaggggtg atcaaagcgc aaatcacggg caaaaccact gcgcggatca gcggccctac
     6061 agggctataa tcacgggcga tttgtcggtc gagaatgcca atgccctgag tgcgttgttc
     6121 catattcggc gtagcaagca gcatctctac cagctccgac acgtcggcgc gcatctcgcg
     6181 cactagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa caccaccaat
     6241 cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccatcg tcatcgactt
     6301 ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaaa ccaacaaaat ggcaggagag
     6361 gccaaggttg agcaaatgac tgtgtagccg ctcgacttcc agcagaatcg agcgaatagt
     6421 atgtgctcgt tgcggcacct caatccccag tgcattttca accgagttgg tataggccac
     6481 actgtgggca aaaccgcaaa tcccacacac gcggtccgat aagaaggtca cttcgttgta
     6541 gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca ggcggtaatc
     6601 agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt cataggaggt
     6661 gatatgcagc gggccgacag ggatcacccg cgcatcgctg ttgccctcat tgataaacgc
     6721 atacgtttcg gaatctgtcg tcggttcagg gcgcagtcga taatccatcg catctttgcg
     6781 cagcggatgc atatcttccg gccagtcatc gggcaacacc aggcgacgct gatccggcag
     6841 gccaaccgga atcagcccgt acatatcgcg aatttctcgc tcgccccaga ccgcggcagg
     6901 gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagtg ccttcaccac
     6961 gatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt aatgaccgtt
     7021 aagtgtccgc tcgtcgttgc caaacaggac tggaagccag ccatcatgtt gataataaag
     7081 ataatgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct gttcaggcgt
     7141 ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg cgagaatcgc
     7201 ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca gatgcggtgc
     7261 ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc gtccctgaga
     7321 ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata tgcgttccca
     7381 tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag ccgagatcac
     7441 cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgcagccatc cgtaccaggc
     7501 ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg tgattacgtg
     7561 ccagtcctgc ggtaacggtc ataaattcgc taagaaaaat gttgaagggc ggcatccctg
     7621 ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt ttgagtatcc
     7681 cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg gaaccgcaga
     7741 acagcagcgt ttttgccaga ctgtggttta aggtgtgcag cagcgcggca aaaattccca
     7801 gcggcccgcc aatgcctagc gccaccgcga ccagccccat gttctccacg ctggagtacg
     7861 ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg gcaaccgaca
     7921 acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggcc tggcaaataa
     7981 tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg gagagcaggg
     8041 cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc atgggaaata
     8101 gcccggtttt ggtaccaaag ccaattagca caaacacaaa ggccaacagc attaatgttg
     8161 ggtcaagcaa ggacgattgc ttaagaacct cgctccagaa tatcgccatt tctgcctgcg
     8221 gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca aaagcgacac
     8281 caacagtaca aataatgatg tacttccatg ctgattccag cgatgaacgc tgaccgtaaa
     8341 tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac atcacaatca
     8401 ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag aaaccgtagt
     8461 aatcgcacag tgtaacgggt gaaagctcgc cgcgtgccac ttcatgacgc atgtagccaa
     8521 tcgagtaaac accggtgaga aagccaatca caccaagaat ggcgaggaac aaaccgccca
     8581 gaccatcaat atgaagccac agtcccgcag cgaatatttc tcctgcatca gcggcagttt
     8641 ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt aatacagtca
     8701 ccgtacaagt cgcagagagc ccccgtttcc ggcaggcaaa acagagcagc gaaaaaagca
     8761 gcggcgtgag caggagtaaa gcgaacatca cagaataact catctcatta tcccttcagc
     8821 gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct tgccagtaac
     8881 accatcacaa tgacggcgaa gatggcgtcg gtagcgatac ctatttccac cagttccggt
     8941 gctcgccagg caagaagcgc cagcaccaga tgggagccgt tttccatcag gcagtaacca
     9001 aaaatttgcc gcaggatatt gcgctggctg acaatgcaca gcaggccaag cagaaaatga
     9061 cctaacgcta ccgccagcgc cggtttcagc ccggtagcca tcggtagctt cacgggctga
     9121 acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgccatcat tgccggaccg
     9181 aataacgctt tttccgggat gttctgggga atatttcgtg cagcgtaagt catgattaac
     9241 ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag ttgctctgcg
     9301 gcgaacaggc acgagagagt ggcgaaaata gacaccagca ccagcgactg gcaggcgtaa
     9361 aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagcgatgt cagcatcatc
     9421 agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct tactgcaacc
     9481 aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc aacaccaggc
     9541 gcattgaccc tagcagcggt gcggcatcgg cgacggcctc gctcggtttg cccggcacga
     9601 cgcgaccgaa ccagcgaata aaccaggcga aactggcgac cgattcaatc atcagcagaa
     9661 tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccggcg gcaaacagcg
     9721 ggaatttact gaagaagccg ttgaacggcg gcacaccggt aattgccagc gctgccacgc
     9781 agaaacccac gcctggcaac ggcagggtgt gcaatacgcc acgcagacgc ggcaacaagc
     9841 gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa aaacaggctt ttagcgaacg
     9901 cgtggttgac gatgtaggcg atgctaccct ccagcgccag ccgcgagccg aagatggaga
     9961 gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc agcaaccgct
    10021 tcatatcctg ctgtggcaaa tacatcagaa agccataaag aatggtgacc agtgccatga
    10081 ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg gcgcgggcaa
    10141 aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcgctg atcggtgttg
    10201 gcgcttccat tgcgtccggt agccacgctt gcatcggtag ctgggccgat ttcccccacg
    10261 cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccatgtaac tcgctcatcg
    10321 cgctaagcac aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga tacaacccca
    10381 acgaaccgat atgggtgata agcaacgctt ttagtgctga acgctgcgct ttatcgctct
    10441 ggtaataact gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac aacaactgac
    10501 cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc agcaaaaatg
    10561 cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc agataacccg
    10621 tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc agcgtactca
    10681 cgcgatcaat gaccaggcca aacaatgcca catcccccac gttgaccaac gtcaacgtga
    10741 cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac agcgtggtca
    10801 gcgcggcgaa caaaaccccc cattcggcgg cccgacgttg tggcgaaaac gaaacgacca
    10861 gtgcgccgat aaaaggcagc attaacgtcg tcagagcaag attttccata ttccgtcagt
    10921 gctccttaca gaccggttaa ccagaagacc catgcaagcg cagcaaggct gaagccaagc
    10981 caggtcacat ggtgaatgag taaaaaacgc ccgcgtgcca gcgtgttttc tgcgattgag
    11041 gccagtacaa aaatcagcaa aaccttgagc agcgtaacga caagtgaagt cagcaggcag
    11101 gcgagagaaa gttcttgcgc gcgcccaaag ggcagaaaca gggccacaaa cagtgctgcc
    11161 atcacgacct gtttcagccc cagcccccat ttcgctagcg ccagcccggc accggaatat
    11221 tcggtcagcg ggccttcctg taattcctgt tctgcttcag caacatcaaa gggaattttt
    11281 cccatctcaa tgaagcaggc aaaaccacag gccagtaacg ccagtacggt ggttagcggc
    11341 gagttccagc ccatcgccag cgtattgctg atcatctcga tatgcgtgga acctgctatc
    11401 agcgccaata ccagcagtga gagaataagc attggttcga ccagaatgcc gagcgtcaac
    11461 tcgcgactgg caccgactcc cgcaaacgga cttccggtat ccagcccgga aagagcaaag
    11521 aaaaaacgaa acagggcaag aagatagata agggtgatca gatcgccgcc gcccgcaaaa
    11581 ggggaaacgg taataaatag tggtaaggcc atcgccagca ccagcatgct gctgattaat
    11641 acccacggca tcaggcggaa catcagacct gaagatgtcg gcgcaacttc ctggcgttta
    11701 aacagtttgt ggatatcgcg ataatcctgc cagatccccg gcccgcggcg ggagtgcata
    11761 cgcgcgcgta tctgccggga aatacccgta aaaagtgggg ttagcatcag cagaatcacg
    11821 gcctgtgcta acgcaaaaat gaccagatat ccgtcgcaaa gagtttgtct catggtgatt
    11881 tccttagacg gcaatagcga ttagcagcac aaccagtgcg gcgaccacat acaggcaata
    11941 gagacgaaag tcgccgcttt gcagatgctg tatttctttg accagccgtt gggtggcgct
    12001 cacgatgggg cggatcaccc gctcatccca gaagggttct gtgctctgag ccctggcggt
    12061 gacgtgcgca agacctttat ttagcctcag cgtagggtcg agctgttgtc gtagacgaaa
    12121 taacgcagag aagaccacac gcagcggctg catcacgcca ttgcctgacg gagccatcgc
    12181 gttttctcag ccgtagccgc atgcccaggc atctcctgtg cgacgaaacg ctgcgcggcg
    12241 cgaacgacac cacagccagt aaaggccagg cagcaaaggc atcgccagta gtaacagcaa
    12301 cagtaatgaa ggagtgaccc gtgtatgaaa cgtgccgggt acaagtgcta ttccgctggc
    12361 gacagtgacg ggaggggtat tggtaaacgc atgggcaata tgcattatct tcggtgccag
    12421 ccaactggca ctaagcgcaa ttaatacgca gagtgccgcg agcagtagca tggcgacgat
    12481 catcgtattt ggcacttcct gtgcctcttc agcgtgtgta ctgcgcggcg caccacagaa
    12541 agtaataccg tacattttga cgaagcacat tactgccagc ccaccagtga ctgccagcat
    12601 taccagagca ataggacccg caagttgtag cgctacggct tccacacgac ttagtgagaa
    12661 cagcgactgc caggtgtacc attcgctgat aaaaccattc agcggaggaa tggctgatat
    12721 cgcgaggcaa ccaatcaggc atgctgcggc tgtccacggc atccgtttcg ccagtgcccc
    12781 cattttttcc atgtcgtggg tatgcaaacg cgaaataatc gcgcccgcgc cgagaaatag
    12841 cagccctttg aacagcgcat ggttgagcag atgaaacagt gcgccgagca gtccaaccac
    12901 ggtgagcagc gggtcgtgca gtgacagacc gtccatcgcc acaccgactg ccagcaaaat
    12961 aatgccgacg ttttcgacgg tactccaggc cagcagccgt ttgatatcct gttccgccag
    13021 cgcatatagc acgcccagga gcgcggagat tgcgccgatc gccatcacca gaatgcccca
    13081 caacagaggc aaacccgttt gcgccagcag atccatcgct actttcagga tgccgaaaat
    13141 accgattttg accattacgc cagacatcaa cgccgaagcg tgcgatggtg cggcagggtg
    13201 agcgcgcggc aaccagctgt gcaacggcat catcccggct ttcgcggcaa aaccgaaaaa
    13261 ggccagcaga aacaccgccg acgccagccc cggagaaagt gaaagcgtgc gaaactggca
    13321 aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcataat cagcaccgat
    13381 ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt atcctgcctg
    13441 gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat gaaccaaaaa
    13501 gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaagattcat aaagaagccc
    13561 atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata gagcgaacaa
    13621 accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc cattcgcagg
    13681 gttaaatcgg caaagtcaaa aggggtggca aaatgggcga ctaaagttac accagcatgt
    13741 aattgcgtaa ttccgctgat gatgccaatc acgccgcaca ccagcgatgt gatgccggaa
    13801 agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact ggcaaacaga
    13861 tagaggatca gcgaccaggt taataattgc agggcatcca ttagtgttgc tcctgagtga
    13921 gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc tgtttttctt
    13981 tcatctgacg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc gggcaagcgc
    14041 gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc gcgacggtct
    14101 ggacacccgg ttcccagcgc agcaacgcat gttggggcgg cacgttttct tcgccgtctt
    14161 tgagtgagcc ttcagcctga aaaacatatt gcgcatgggc attcaccgga cggcttcctg
    14221 aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca atacagaggc
    14281 tttcgttgag ttggatcgca tcatccctct gagagatggc attgaccggg cagacctgca
    14341 ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgattgtt gacgtcttcg
    14401 ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc gaacaggcag
    14461 cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag cggttcatgt
    14521 cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagga tgccattctg
    14581 attaactcaa tctattcaat aagttatgta ttaactggat tctgaacggt acaaaaatga
    14641 tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg aaagcgagaa
    14701 tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag cacggaatgg
    14761 agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg tgattgctgg
    14821 ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg aagctgatgc
    14881 gatgaatacc atcgtaggtt ttgcccatga aggacttttc accccagacg ccgaattgtt
    14941 cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct ttttccgcaa
    15001 aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg cccgcttttt
    15061 tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg ggggtcatgg
    15121 ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa ttaacttgtt
    15181 ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc agtggattca
    15241 ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa tactgccttg
    15301 tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg cgtccgcaga
    15361 tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat tttcagcagg
    15421 ttgtgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa gtgctgtgaa
    15481 gcgttcaatt aaatgcgggg agtctgcaac atcgacctga acccagacag atgctggcat
    15541 tggcggacgc ggacgcgccg tcgtgctctt catcacgatt aaaagatcca gttcggcacc
    15601 tttcaacggt aacgttgatt caatcagagt gatggcattc catgaaccgg aaagcagcat
    15661 gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat tacagccgca
    15721 actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac ccaacgcagt
    15781 gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa ggggtgagtt
    15841 gttcttaagg aaagcataaa aaaatcatgc atacaacaat cagaacggtt ctgtctgctt
    15901 gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag gatggcccat
    15961 gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta atgtctgtcg
    16021 ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg cgatcgtttc
    16081 tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg atgtggtgat
    16141 gatgacgctg gatctggctg atgggcgcat tccggtaatt gccgggaccg gcgctaacgc
    16201 tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg tcggctgcct
    16261 gacggtaact ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc atttcaaagc
    16321 catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc gtactggctg
    16381 cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta tcggaatcaa
    16441 agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt cagatgattt
    16501 tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattgg gcggtcatgg
    16561 ggttatttcc gttacggcta acgtcgcagc gtgtgatatg gcccagatgt gcaaactggc
    16621 agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc cattacacaa
    16681 caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg aactgggtct
    16741 tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg gtcgtgagac
    16801 ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat ttgatggctt
    16861 actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt ttattactcg
    16921 ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa gcctacctgg
    16981 aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg gtgacctccg
    17041 gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg ctggacattc
    17101 gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc acgggcgata
    17161 ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag gtggttagcg
    17221 tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag acactgacca
    17281 ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt cgttatcaaa
    17341 tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgctg aacctggaac
    17401 aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg gagatgatga
    17461 acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct gcgcaaaatc
    17521 gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta ccaatgctgg
    17581 tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg gaaaaagtgg
    17641 gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact tataagccgc
    17701 tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca tccggtgact
    17761 ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc gatccgaaag
    17821 gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag gctgcgttta
    17881 gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatataa tacgcaaacg
    17941 tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg tttgtcggac
    18001 gtagttcgga taaggcgttc acgccgcatc cgacaaaaca tccggcacac cagacagcaa
    18061 aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc aagctgagtt
    18121 gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt tggtgctcga
    18181 attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg atcgcaaagg
    18241 tatggtgaac aacaagttca actacttcat tatgagcaaa ctggctgaag cgggtatccc
    18301 gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc tggatatggt
    18361 gccggttgag tgtgtcgtgc gtaaccgtgc tgctggctct ctggtgaaac gtcttggaat
    18421 cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa acgatgccat
    18481 gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga gcaaagagaa
    18541 cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa aactgttcga
    18601 tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca aaggtgaagt
    18661 ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag aaacactgga
    18721 gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag cctatgaagc
    18781 cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat cttgccgtgc
    18841 agctggcacg gcaagacaac cgctctcgga atatatgcat tgaacgctca ttcctccgcc
    18901 atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca tttctataat
    18961 gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga caatgttgaa
    19021 gacaggcgca acagctctgg cggtccgtca atgggcggtc ccggtttccg tctgccgagc
    19081 ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta tggcgttgat
    19141 ttaaccgggt tgatgaccgg gcagccggtt tctcaacaac aatcaacgcg gtcaattagc
    19201 cctaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac ggaagacacc
    19261 tggggacaac agttcgaaaa gatgggtaag acctatcagc aaccgaagct ggtcatgtac
    19321 cgtggaatga cgcgcaccgg ctgcggggcg ggccagtcca ttatggggcc gttctattgc
    19381 ccggcggatg gcacagttta tatcgatctc tccttctatg atgacatgaa agacaaactt
    19441 ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg tcatcatgtg
    19501 cagaaactgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc gacgcaggcg
    19561 gaagtaaacc acttatctgt gcgtatggaa ctccaggccg actgttttgc cggtgtctgg
    19621 gggcatagta tgcagcagca aggcgttctg gaaactggcg atctggaaga ggcgctgaat
    19681 gcggcgcagg ccatcggcga tgaccgttta caacagcaaa gtcaggggcg agtagtacca
    19741 gacagtttca cacatggcac ttctcagcaa cgctacagct ggtttaaacg tggtttcgac
    19801 agcggcgatc cggcacaatg caatactttt ggtaaaagca tttaattttc ggggcaggga
    19861 tggctgaact gactgcgctt tacacattaa cagcgcaaat gaaacgtgaa gggatctgcc
    19921 gcttgctggt gttgagcggg gaagagggtt ggtgttttga tcatgctctt aagttgcgtg
    19981 atgccttacc tggcgactgg ctgtggattt cgccgcagct agatgctgaa aaccactgtt
    20041 caccctcggc gctacaaact ttacttgggc gcgagttccg gcatgcggta ttcgacgccc
    20101 gccacggctt tgatgccgct gcctttgcag cacttagcgg aacgttgaaa gcgggaagct
    20161 ggctggtttt gttactccct gtatgggaag agtgggaaaa ccaacctgat gccgactcgc
    20221 tgcgctggag tgattgccct gaccctattg cgacgccgca ttttgtccag catctcaaac
    20281 gcgtacttac ggcggataac gacgctatcc tctggcggca aaaccagccg ttctcgttgg
    20341 cgcattttac tccccgtact gactggcacc ccgcgaccgg cgcaccacaa ccagaacaac
    20401 agcaactctt acagcagcta ctgaccatgc cgccgggcgt ggcagcggta acggctgcgc
    20461 gtgggcgcgg taagtcggcg ttggcagggc aactcatttc tcgtattgcg ggcagtgcga
    20521 ttgtcaccgc gcccgcaaaa gcggcaacgg atgtactggc acaatttgcg ggcgagaagt
    20581 ttcgctttat tgcaccggat gccttgttag ccagcgatga g
//
LOCUS       DADLSA010000064        20528 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.064, whole genome shotgun sequence.
ACCESSION   DADLSA010000064 DADLSA010000000
VERSION     DADLSA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.064"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..686)
                     /locus_tag="JN691_002318"
     CDS             complement(<1..686)
                     /locus_tag="JN691_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY7243747.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARG"
     gene            860..1573
                     /locus_tag="JN691_002319"
     CDS             860..1573
                     /locus_tag="JN691_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY7243748.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            1605..2363
                     /locus_tag="JN691_002320"
     CDS             1605..2363
                     /locus_tag="JN691_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243749.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            2409..3758
                     /locus_tag="JN691_002321"
     CDS             2409..3758
                     /locus_tag="JN691_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAY7243750.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            3758..4582
                     /locus_tag="JN691_002322"
     CDS             3758..4582
                     /locus_tag="JN691_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243751.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            4594..5153
                     /locus_tag="JN691_002323"
                     /pseudo
     CDS             4594..5153
                     /locus_tag="JN691_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
     gene            5184..6254
                     /gene="lptF"
                     /locus_tag="JN691_002324"
     CDS             5184..6254
                     /gene="lptF"
                     /locus_tag="JN691_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY7243752.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYNVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            6251..7242
                     /locus_tag="JN691_002325"
                     /pseudo
     CDS             6251..7242
                     /locus_tag="JN691_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
     gene            complement(7278..8450)
                     /locus_tag="JN691_002326"
     CDS             complement(7278..8450)
                     /locus_tag="JN691_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY7243753.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDVIIQAFATLKQ"
     gene            complement(8450..8698)
                     /locus_tag="JN691_002327"
     CDS             complement(8450..8698)
                     /locus_tag="JN691_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7243754.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8730..9626)
                     /locus_tag="JN691_002328"
     CDS             complement(8730..9626)
                     /locus_tag="JN691_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAY7243755.1"
                     /translation="MTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNLTWVRGS
                     LEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGFCQRFLF
                     ISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKPLFDWML
                     RGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDWQRVRQL
                     AAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADWSASNNR
                     ISEDINWFPGISLEHALRNGLF"
     gene            complement(9641..11328)
                     /locus_tag="JN691_002329"
                     /pseudo
     CDS             complement(9641..11328)
                     /locus_tag="JN691_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            11732..12874
                     /locus_tag="JN691_002330"
     CDS             11732..12874
                     /locus_tag="JN691_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7243756.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQDFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(12998..13768)
                     /gene="glcC"
                     /locus_tag="JN691_002331"
     CDS             complement(12998..13768)
                     /gene="glcC"
                     /locus_tag="JN691_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HAY7243757.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            14019..15518
                     /gene="glcD"
                     /locus_tag="JN691_002332"
     CDS             14019..15518
                     /gene="glcD"
                     /locus_tag="JN691_002332"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HAY7243758.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVDVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            15518..16570
                     /gene="glcE"
                     /locus_tag="JN691_002333"
     CDS             15518..16570
                     /gene="glcE"
                     /locus_tag="JN691_002333"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HAY7243759.1"
                     /translation="MLRECDYSQALLEQVNQAISDKMPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLLGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            16581..17804
                     /gene="glcF"
                     /locus_tag="JN691_002334"
     CDS             16581..17804
                     /gene="glcF"
                     /locus_tag="JN691_002334"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HAY7243760.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTFKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            17809..18213
                     /locus_tag="JN691_002335"
     CDS             17809..18213
                     /locus_tag="JN691_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HAY7243761.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            18235..20406
                     /gene="glcB"
                     /locus_tag="JN691_002336"
     CDS             18235..20406
                     /gene="glcB"
                     /locus_tag="JN691_002336"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HAY7243762.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
BASE COUNT         4967 a         5109 c         5522 g         4930 t
ORIGIN      
        1 taaccacgcg cggtcataaa atccgtaatc gctttttctg catcaaccaa cacctgttcc
       61 agtggctgat tggcatcgat atcaaccagt tgtgcaccac caaaggttaa ctgtggcgtt
      121 atggcaatct tcctcgccag cgattcccgt ttatggtcgg gtttacgtgc acaggcaact
      181 tcaaggtcaa cattgagttt gatgaccaga tcaggcttat ggctcgccat ccagtgaaac
      241 gctttacgtt cctggcttgc cagccatgag acaaaacgac caccttcaac gttaggtggg
      301 aacaccgtac catcgtaagc gccaggaatt tggtcctgag gataacggtc ggttagaaca
      361 attaacccgc gacgacgaca ggcaagcata tgacgaaagc gcagtaagcg acgggcgaca
      421 aacgctgtaa ttaccagcgc cggaactggt ccaggcaatt tttttgctgt tttcacctga
      481 tttcgttcaa ttgttttatg taaggatttt cccatcaaag gtaattttgt cactgcacga
      541 ccgacatttc cagcctgttt tcctaaatga actctttcgg cagcaccata tttttcgaca
      601 acggtaataa gatgttcaca caccgttgac ttgcctgaac catcgctacc aataacggca
      661 attaatggag gtgttattga ctgcatgaat atatccttta atttaaatat ccattaaaaa
      721 tatttatttg gttaatatgt ttttatgaaa gcgtaattca ggtcaatgtc acaattaacc
      781 attgtcacaa taaggttgaa cggatattct gagcacggac cacgtaatat tcaatacatt
      841 atttactgcc gggtttttcg tgaatcaacg taatatgtca ataattaatt ccacgccagt
      901 gcgtgttatt gccattgtag gatgtgatgg ttcaggtaaa tcgaccctca cggcaagcct
      961 ggtaaatgaa ctggcagcaa gaatgccaac agaacacatt tatctcgggc aatcgtccgg
     1021 gcgaattggc gaatggattt cacagctccc tgttattggc gcaccttgtg ggtgttatct
     1081 gcgaagtaaa gcggcacatg tacacgaaaa gccctcaaca ccgcctggca atattactgc
     1141 gctggttatc tatctgcttt cctgctggcg ggcgtacaag tttcgcaaaa tgttgtgtaa
     1201 aagccagcaa ggctatctgc tcatcaccga ccgctacccg caggttgaag tgccgggttt
     1261 tcgctttgat ggcccgcaat tggcaaaaac cacgggcggt aacggttgga taaaaatgtt
     1321 aaggcagcgc gagttgaagc tgtaccaatg gatggcgtct tatttacccg tattgttgat
     1381 tcgtcttggc attgatgaac aaaccgcgtt tgcgcgtaaa cctgaccacc aactggcagc
     1441 gttacaggaa aaaatcgccg ttacgccgca actgacattc aatggcgcca agatccttga
     1501 actggatggt cggcaaccag ccgatgaaat tatgcaagcg tcactacgcg caattcacgc
     1561 cgcgctttcc tgatcaccaa aatggatagg ggatgattta acgaatggat gcactacaaa
     1621 cacaaactgt taatagtaca accgcgccgc agcccaacta cattccgggg ctgattgcgg
     1681 tggtcgggtg tgatggcacc ggtaaatcca cgctgaccac cgacctggtg aaatcgctgc
     1741 aacaacactg gcaaaccgag cgccgctatc tggggctgct ctccggcgaa gacggcgaca
     1801 aaatcaaacg attgccgttg gttggcgtct ggctggaacg gcgactggcg gccaaatcct
     1861 cgaaaaccca aagcatgaaa accaaatctc cggcgctatg ggcggcggtg attatgtact
     1921 gcttctcgct gcgaagaatg gcgaatctac gcaaggttca gcgactggcg caaagtggcg
     1981 ttctggtggt cagcgatcgc ttcccgcagg ctgaaatttc gggcttttat tatgacggac
     2041 cggggatcgg cgtcgaacgt gcgaccggga aaatcagcat gtttctggcg cagcgcgaac
     2101 ggcgtttata ccaaaaaatg gcgcaatacc gtcctgaatt aatcattcgt ttggggattg
     2161 atattgatac tgccatctcc cgcaagcctg accatgagta tgccgagctg caggacaaaa
     2221 tcggcgtcat gtcgacgatt ggctataacg gcacaaaaat tcttgagatt gattccagag
     2281 cgccctacag cgaagtgctg gaacaggcac agaaggcggt ttctctggtt gccatcgttt
     2341 ctgaccgccg aagtttaact taaggctggc gggatatttt ttactatcaa cgataaggtc
     2401 aggctgaatt ggcgggtttt aacatcaaac attggtttgc agatggcgcg tttcgcacca
     2461 ttattcgcaa tagcgcctgg ttaggctcca gtaatgtcga gagcgccttg ctggggctgt
     2521 tggcgctctc gtgtgccggt aaagggatga cgcccgccat gtttggcgta ctggtgattg
     2581 tgcaatcgta cgccaagtcg atcagcgatt ttattaagtt tcagacatgg cagctggtgg
     2641 ttcagtacgg aacgccagcc ttgaccaaca ataatccgca gcaatttcgc aatgtcgtct
     2701 cattttcctt ctcgctggat atcgtcagcg gcgcggtggc gattgtcggc ggcattgcct
     2761 tactgccatt cctttcccat tcattaggtc tggatgacca aagcttttgg ctggcggcgc
     2821 tgtattgcac gctcattcct tcaatggctt cctccacgcc gaccggcatt ctgcgtgcgg
     2881 tagatcgctt tgatttgatt gctgtacagc aggcgacgaa accttttctg cgcgcagcgg
     2941 ggagcgtcgc agcctggtat tttgactttg gttttgcggg ttttgttatt gcctggtacg
     3001 tgtcgaatct ggttggcggc accatgtact ggtggtttgc cgcgcgcgaa ttacgccgta
     3061 gaaatatcca taacgccttc aaattgaatc tgtttgagtc tgcccgacac attaaaggcg
     3121 cgtggagttt tgtctggtca accaacattg cccactccat ctggtcggcg cgtaactcgt
     3181 gcagcacagt gttggtgggg atcgtgttag gacccgctgc cgccgggtta tttaaaatcg
     3241 ccatgacatt cttcgacgcc gccggaacgc cagcgggttt gctgggtaaa agtttttacc
     3301 cagaggtgat gcgtttagat ccgcgcacca ccagaccgtg gttgttaggt gtgaagtccg
     3361 gtttactggc gggcggaatc ggtattctgg tggcgctcgc ggtgttgatc gtcggcaagc
     3421 cgctcatttc gctggtgttt ggcgttaagt atctcgaagc gtatgacctg attcaggtga
     3481 tgctgggcgc aattgtgatt tcgatgctgg gcttcccgca ggagtcattg ctattgatgg
     3541 cgggaaaaca gcgcgctttt ctcgtggcgc aaaccatcgc ctcaatcggc tacatcgtat
     3601 tgctgttcat gttctgtcat ctgtttggtg tgctgggagc agcgtttgct tacttcggtg
     3661 ggcaatgtct ggacgtggcg ctctcgttaa ttccaacctt aaaggcattt tttcagcgcc
     3721 attccttgct ttataacgca gccggagaga aatcctgatg atgagaaaat atttcccgct
     3781 tgaggcgagc gagcgtctat ttgttgcgat tgaagaagat gatgtcgttg atgcacaggt
     3841 tagtttgccg ccgactattg cactaagttg taccaccgaa atcattcatg ataattacgc
     3901 gttatgcctg caattctggc tcaatggcgt tgatcgtcag gaattactgc gtttagttcg
     3961 caaacaagcc aaaggcgatg agctgactgc tgatgagcgc aaacaattta aatacatgcg
     4021 agcgcgctac aagcacctgc gttttgcgca acggctgtat ttaaagaagc atcaggccgg
     4081 atttttattc ggcaaaacga ccgtttttct ggggcgtttt caggacggct ttcgtaacgg
     4141 taagaaaaat attgtgtcgt attacggcaa cctgttacgg atttatttaa gctctccggt
     4201 ctggtcgttg gtgaactatt cattacgcca tagccagtta gaaagcgtaa gcagttttat
     4261 tgcctatcgg caaaagcaaa tgcataccct gaaagagatt attgccaaac cgcgactaac
     4321 cggcagggaa tttcatgatg tgcgaaaaat catcagccag caggtttctt attacgacac
     4381 cttaagatcg cttgatcctg aaaataaaga ggcgttacag atttcccgtt tcctcgccgc
     4441 cattaatggc ctgatgggag ataaacacga cgacatggtg gcggatgata tggaaaaccg
     4501 ccagtcgtat gatgcaccgt tggcgttgga tagcgatatt cgtcagcgct tagagctgct
     4561 gatttcgcgt tttccgctat agccggagca atgatgtcat ctcgtttgat tattgcgttg
     4621 attatcatgc tcttagcccc gagtgtgcag gcacacaatt tcgtcactgg caaaacggtt
     4681 acccctgttt atatccagga aggcggcgag ctgcttctga atagcgacga cgaaattcac
     4741 taccaaaagt ggaacagcac acaattagcg ggaaaagtcc gcattattca gtatatcgcc
     4801 gggcgtaagt cggcgaagaa aaagaactca ctgttaatta aagcggttga agcggcaaac
     4861 tttccgcagg atcgttttca gcccaccacc attgtgaaca ccgatgatgc catctttggt
     4921 actggctatt ttgttgttgg caaaatcgaa aaaaataaac gcgctatccg tgggcgcaat
     4981 tcgttattga cggcaatgga cagggacgcg ttgcctggcg cttgccggag cagagttcaa
     5041 ccattctggt gctgaataaa gccgggcaaa ttcagtgggc aaaggacggc agcctgacgc
     5101 cggaagaggt agatcatgtc attgcgttgg cgcagaaact gatcaatgaa taaaaaactc
     5161 aaaggattga gggcgttagt gagatgaagc tggttgaaca ctacatcatg cgtggtacgc
     5221 gccgtctggt gctgattatc gtcggttttc tgatctttat tttcgccagc tactccgcac
     5281 agcgttatct gaccgaagcg gcaaatggga cattagctct tgatgttgtg ctggatatcg
     5341 ttttctacaa cgtactgatt gcactggaga tgttgttacc tgttggtctg tatgtgtcag
     5401 ttggcgtgac gctagggcag atgtataccg actcggaaat taccgctatc tctgcggcgg
     5461 ggggcagtcc gggacgcttg tacaaagccg ttctttatct ggcgataccg ctaagtattt
     5521 ttgtcaccct tctgtcgatg tatggtcgtc cgtgggctta tgcgcagatt tatcaactgg
     5581 agcagcagtc gcagtcggag ctggatgttc gccagttgcg ggcaaagaaa tttaacacta
     5641 acgataacgg acgaatgatc ctttcgcaga cggttgatca ggataataat cgcctgactg
     5701 acgcgctgat ttatacttct actgccaatc gaacccgtat tttccgcgcc cgttcggttg
     5761 atgtggttga cccatcaccc gagaaaccga ccgttatgtt gcacaacggc actgcctatc
     5821 ttctcgatca tcaggggcgt gacgacaacg aacagatcta ccgtaatctg caattacatc
     5881 tgaatccgct ggatcaaagc ccgaacgtca aacgcaaagc gaaatcggtc acggagctgg
     5941 cgcgctccgc ctttcctgcc gatcatgccg aactgcaatg gcgacaaagc cgtggcctga
     6001 ccgcattgtt gatggcgctg ctggccattt cattaagtcg ggtaaaaccg cggcaagggc
     6061 gattttcaac gttattgcca ctgacgttgc tgtttattgc cattttttat ggcggcgacg
     6121 tctgccgtac actggtggct aacggcgcga ttcccctcat tcctggtttg tggttagtgc
     6181 ccggactcat gctaatgggc ctgctgatac tggtcgcacg tgacttctct ttgctgcaga
     6241 aattttcccg atgaatgttt tcagtcgcta tttaatccgt catctctttc tcggttttgc
     6301 cgccgctgca gggctattgc tgccgctttt taccaccttc aacctgatta acgaactgga
     6361 tggtgtcagc ccgggcggtt atcgctggac tcaggcggtg ctggtggtgc taatgacctt
     6421 accgccattc gcagcacggg gttttctatc ttccgtattg cactggtggc gctggttgcc
     6481 gggatattgt ggactgtttc gttaggcgcg atagatgagt gggtggcgtc gccattgcag
     6541 caacaggcgt tgcaaatcaa atcgaccgcc accgcgttgg gggaggacga tgacattacc
     6601 ggcaatatgc tgtgggccag gcgtggtaat gaatttgtga cggtgaaatc gctgaacgag
     6661 cagggccagc cggtgggcgt ggagatattt cattatcgcg acgatctctc gcttgaatcc
     6721 tacatttatg cacgcagtgc caccattgaa gacgacaaaa cgtgggtcct gcatggtgtg
     6781 aatcataaaa aatggctgaa tggcaaagaa acgctggaaa cattagataa tcttgcctgg
     6841 caatcggcct tcaccagtat gaatcttgaa gagttatcaa tgccggggaa tactttttct
     6901 gtccgtcagc ttaatcatta catccattat ttgcaggaaa ccgggcaacc cagcagtgaa
     6961 taccgccttg cactgtggga aaaactgggg caacctatcc tgaccctggc gatgattttg
     7021 ctggctgtgc cgtttacctt tagtgccccg cgttcgcctg ggatgggtag ccgtctcgct
     7081 gtaggcgtca tcgtgggctt actcacctgg atcagctatc aaatcatggt taatctggga
     7141 ttgctatttg cgttaagcgc acctgttacc gcgctcggtt taccggtagc gtttgtgttg
     7201 gtggcgttga gcctggtgta ttggtatgac agacaacatt aaacccgccg tggcgcaaac
     7261 accacggcgg gctcacatta ctgcttcaga gtagcaaatg cctgaatgat gacatcgatt
     7321 tgctcacgac tgtgggcagc atttacgcta cagcgcagca gcgtgatgcc tgcaggcgcg
     7381 gcaggcggca ggataagatt cacatacacg cccagcgaga taagctcgcg ccagatgcgt
     7441 aaaccttcct ctttcgaacc gattattact ggcactaccg ggctaatatg tgaacttaac
     7501 tcataaccca gtttttgcag accgtcatac agatggttgg cgttatccat cagcttttgc
     7561 cgcagctctg gttgtgtggc aatagttctt aacgacgagc gcacggtcgc aatgcaggac
     7621 ggagaaggag aggcggtaaa aatatacggg cggctgccgt agcgcagcac ttccatcgct
     7681 tccgaaccga cagcaaaacc gccaatcgag gccagacttt tactgaaagt accaacgata
     7741 atatccacat catcttcaac gccgacagct tcagccagtc cgcgccccgt tgcgcctaac
     7801 acgccaaacg aatgcgcttc atcgacaatc aaataaccac cgagacgacg tttgatatca
     7861 acaatttccg ccagcggcgc aacgtcaccg agcatgctgt agatgccctc aacgatgata
     7921 atcgcctctt tcgcccgttc accgaggcga accatgcgcc gttccagatc tttagcatcg
     7981 ttatggcgga aacgaataat ttccgcgcca ccgagagaac aggcgtcata aatactggcg
     8041 tggctgtcgg catccagtaa caccacggcg ttatggtcgg caagtgcgct gatgacgcct
     8101 aaattagcgg tataaccggt tgaaaaaacg atagcggtag ggcgattgaa aaactcggca
     8161 atctctttct ctaacgccag gtgcggcgca taactgccgt ttgccatacg agaaccggtg
     8221 gtgccggtgc cttgggccgc taacgcggcc tgaccttcgc tgatggcatc atgattaaag
     8281 gttaaaccga gataattatt ggtcccggcc agaatcactt ttttattgcc gatccggcct
     8341 tcggtggcgg aatacacttc atcaatacag gtgccaaacg gtgttaaccc gttgtcacta
     8401 aattgcagac gctcgccagc aaggcgcgca tatttatcgt atagccccat tatttattct
     8461 ccagccatgg aagtagagtt tccattaatt gcgcaggggt tttgacatcc agcagaatat
     8521 taataggaat ggaaatatcg aatctgtctt ccagcatcat taagagatcc atcactttga
     8581 tcgactccag gccaagatcg ttgacgagat cgctgtcggg cttaatttcc acaccgtttt
     8641 cgaccaaatc ctgtaaacag gaaaggatgt aatccattac tatttcacga tttaccatag
     8701 aaacgttaac acacctttaa acaagacaat caaaatagcc cgttgcgtaa tgcgtgttcc
     8761 aggctaatcc cgggaaacca attaatatct tcagaaatac ggttattact tgccgaccag
     8821 tcggcgtggg ttaattcacg aattttcgag cgggtcagca taggttcttt acctgccagg
     8881 cgactcaacg cggtactgat atccgcaagg cctgtgagta ccggcagagg aatgtcaacc
     8941 attcgcacgg aaccacaacg ggctttggcg gcaagctgtc gtacgcgttg ccagtcatag
     9001 ccaccggcga cgccatcgca taattcatag gtttgcgtct gtacagtttc ggcgcttaac
     9061 cactgaccca ctgcttgcgc aaaatcggtg acgtgaagaa aagagagttg tgtatctggc
     9121 gcaccaagtc gtggcagcag gccgcgtagc atccagtcaa acagcggttt taactcttta
     9181 tcgccgggac catacacggc tgtcgggcga aaaacgccca gcgtaatttc gtccgccatt
     9241 gctgccagtc gttgctcggc gacgtatttg gattttgcat accaggagag ctcgggatgg
     9301 cgcgccgcca gcgaagagat aaacagaaaa cgttggcaaa agccgctctc ttttgctgct
     9361 tgcatcaggc gcaggctgcc gtcaacgtta cagcgggtga aaatctcttc tttgtgcccg
     9421 cgcacttgcc cggcgcaatg gaccactacg ctggctccgg caaccagctc gctaagtgaa
     9481 tgagtatctt ccagcgaacc gcgcacccag gtgagattat cattgacgtg agcgcgggca
     9541 gtacgcgtca atgcgcgaac gtgaaagccg cgggcgagca ggttatcgat aatatattta
     9601 ccgataaacc cggtagcgcc cgtcaccgcg acagtttgat tcatgccagg gattcctgca
     9661 cattaagact ggcagcataa gccttctgat aacgtttttt cgcttccgca cgggcaggct
     9721 tgccggagga cgttcgggga atactgtggg gcggcaacag atcgatagcc gcggtcacgc
     9781 caaattcgct ttggatccgt gccgccagcg cgtggataag ctgcccgcga cgttcttcgt
     9841 cgctgatccg acactggatc tgcaaaatga ttttttcctg ggcggtaaca aaagcaatcg
     9901 catcgccaga atgaatttcc ggttcctgtt ccgctatata ttcaatatcc tgcggccaga
     9961 tattacggcc acgaataata atcagatctt taatgcgtcc ggtgacatac agataaccgt
    10021 ccagcagata accgaggtcg ccggtgtcta accagcccgt cgcggcaatc tcgtcttgcg
    10081 aaacctggtc gccaaagtaa ccgctcatca gactgggacc ggagatgcaa atatggccta
    10141 ccacacgttc cgcgaccggc atacctgctt cattgcggat ttcaatacca tgttccggca
    10201 acgctttgcc gcagttgacg aaagtcgata cggcgcgtgt ctctgcaccc ggcgcgacgg
    10261 ctttaccctg atattcgagg atgtcgcgat ccacttcgtt aaccacaacc ccggaggctt
    10321 catcagagaa gctgacagcc agcgcatttt ctgccagtcc gtagcacggc atgaaagttt
    10381 tattgtcaaa gttaacctga cggaaacatt cagcgaattg atggagttgt tctgcggaga
    10441 tcggctcagc accaatacca gcgacgcgcc agcaggaaag atccagttca gcgagatctt
    10501 tttcattcac gcggcgttgg cacaattcat agccaaacgg cggcgcaacg gaaacggtgc
    10561 cgcgattttt actgatcaat ttaagccatt gcaaaggacg catggcaaaa tcctgagtgc
    10621 gcaaataatc tactgaaagt tgcgtggcga cggggtcagg agaaagccga ccagtcccat
    10681 atcatggtag aaaggctgcc aggagacgca gcggtcgcta gggcgtaatt taatgccatc
    10741 gtggcttata gcacgtagat tagccatcac ttcgcgatgg gggataatga cgccacgggg
    10801 aaaacgggtg ctgccggagg tgtactggag gtaggcgata tcgttcggaa ctggacgctg
    10861 gagcgcaaca tcggcttccg gtaatgcctt aaaccaagcg tggcttaaaa catgtaattc
    10921 ggggttgtta tgcgtcgcgg cattgaccag tggcaaccac tcatcaccag tgataatggc
    10981 tgcgggctgg caacttgcta gtaaaccctg gagtttggcg ctccaggaat cccgctgacc
    11041 gacgcccatt ggaatcgcca acgggacggc gactaagcca gcatactgac aggcaaaaaa
    11101 agcctctaca aacccgctac tcgtttcggc aatcagcgcc acgcgttcgc cttttttcag
    11161 gttcagcgat aacaaccgct tcgcaccagc ttcggcacgc gtttttaatg tctgatattc
    11221 cagttgatct tcaagttggc aacgtctgtc ataaaaattc attccggcgc tactcagagc
    11281 ggcgtagtcc aaagcatcaa ccagcgttgg aaaatcggca tagcgcatag gtagggaatg
    11341 cgtaaagatt ttattagaca tatacaccac gaaaaaattg gatatcaaaa ataaaatgac
    11401 aataatgcgg tggattatat aggcataaaa gataaatgag atagtatttt catcggtgtg
    11461 aaaatgaaca ttgatttaaa taatgagttt aagttttttg tttttattat gaatgatatt
    11521 taatttatga atatattaag aagaagcctt gtccggtcgc cttaaatcga caaaaggcat
    11581 tgaggcgttt ctgtgtagcc tctgacctta tcataatcat taattaatac tatttattcg
    11641 tcgtttgtat tcattgagtt gattattaag atgccgctcg ttgctgttgc aacctgtttt
    11701 tcaccattaa ttatttgtta tccacagaga tatggaatgc gatctgctga tgattaaaat
    11761 tgaaaaagta attaataaaa atgaccttaa ggcatttatc gcttttccgt catcacttta
    11821 tcccgacgat ccaaactgga tacccccttt attcattgaa cgcaacgagc atttgtctgc
    11881 gaaaaatccg gggacggacc atatcatctg gcaggcgtgg gtggcaaaaa aagcggggca
    11941 gatagttggg cgtattaccg cgcaaataga taccttgcac cgtgagcgtt acggtgaaga
    12001 taccggtcat ttcggcatga ttgacgccat tgatgatccg caggattttg ccgcgttgtt
    12061 tggcgcagcg gaagcgtggt tgaagtcaca aggtgcaagt aagatcagcg ggcctttcag
    12121 cctgaatatc aatcaggaaa gcggattact gattgaaggt tttgacacac caccctgtgc
    12181 gatgatgcca cacggcaaac cgtggtatgc cgcgcatatt gaacaactgg gttatcacaa
    12241 aggcattgat ttactggcgt ggtggatgca gcgaaccgat ctcactttct ctccggcgct
    12301 aaaaaaactg atggatcagg tgcgcaaaaa ggtgaccatt cgctgcatca atcgtcagcg
    12361 gtttgccgaa gagatgcaga tcctgcgtga gattttcaac tctggctggc agcacaactg
    12421 gggatttgtg ccgtttaccg aacatgaatt cgcgaccatg ggcgaccaac ttaaatatct
    12481 ggtgccggac gatatgatct acatcgctga gattgattct gcaccctgcg cgtttattgt
    12541 cggcttgcct aacatcaacg aggcgattgc cgatctgaac ggatcgctct ttcccttcgg
    12601 ctgggcaaaa ttgctgtggc gtttgaaagt cagcggtgtg cgaactgcgc gagtaccgct
    12661 gatgggcgtg cgcgacgagt atcagttcag ccgcatcggc ccggtgattg cgctgttatt
    12721 gattgaagcc ttacgcgatc cgtttgcccg ccggaagatc gatgcgctgg agatgtcatg
    12781 gatcctcgaa accaatacag gcatgaataa catgctggaa cgcattgggg cggagccgta
    12841 caagcgttac cgattgtatg aaaagcaaat ttaataccag atcctgttta gcgcaggggc
    12901 ggtcattcgg gagagggtat agatgaacag atttaacacc agatcctgtt aagtgcaggg
    12961 gcggtcccct cgccttgggg atagggtaaa actctgctca cattcccacc ttgttcatct
    13021 ccagcggcac cgccgagcga atcaaatggt ggccttccag ctcgatatcg tggagattct
    13081 ttttcacggt ccgtacatga tcgcgcgctg cgcgctgagc gacgtgcggc agccgctgca
    13141 acaccgcgtt gtagatccgc gcatgctggc gatcgatctg ctttttttgc tgtggtcgat
    13201 ggtagagatt atttacgctg gcaaacactg aattaaacat cagatcggtc aatgattgca
    13261 gcgtaaacac cagcacctga ttgtgagaag cctgacaaat ggcgagatgg aaagcgtgat
    13321 ccaactgcgc atgttcgatc agcgaaatct ctttgttgtt ctcactggcg gcgagcattt
    13381 tttcataaca gcgggttatc acaacaaaat cagcctgcgt tcccagcgtt gccgccagcc
    13441 ttgccgattc gccctccagt aatgcgcgaa cgtcgagcag atcgtacagc gttcgcggct
    13501 gcgtactgaa cagatggatc agcgggctgg tgtcctgcac ccgattaagc cgtgcgacac
    13561 gagaatcgcg accctgcgcc gtttcaataa tcccgcgccc gcgcagcacg gtcagccctt
    13621 cacgcagtgc ggagcgtgag aagccgagct tttcacacag tcgacgttcc gagggaagcg
    13681 gctgaccgac cttcagtacg ccgtcgataa ttaaccgttc gatactctct gcaaccactt
    13741 cgcaaatagg gcgacgttca tctttcattc ccggacctcg tgcacaggta ggaccaattt
    13801 ttctgttctt aactcgcaaa acacgcacat cacgtaagtg attatgttac atcaatttaa
    13861 cattaagtta accaagacaa ggtcacagag ctggaaaaaa aatggtctga ccggtagcta
    13921 aagagataga cgaaaacgaa aagcccgctt aataactgtt cacagaagca gcgcgcaaaa
    13981 atcagctgcc acacaacaca acaaagcgaa gcctactcat gagcatcttg tacgaagagc
    14041 gtcttgatgg cgctttaccc gatgtcgacc gcacatcggt actgatggca ctgcgtgagc
    14101 atgtccctgg acttgagatc ctgcataccg atgaggagat cattccttac gagtgtgacg
    14161 ggttgagcgc gtatcgcacg cgtccattac tggttgttct gcctaagcaa atggaacagg
    14221 tgacagcgat tctggctgtc tgccatcgcc tgcgtgtacc ggtggtgacc cgtggtgcag
    14281 gcaccgggct ttctggtggc gcgctgccgc tggaaaaagg tgtgttgttg gtgatggcgc
    14341 gctttaaaga gatcctcgac attaaccccg ttggtcgccg cgcgcgcgtg cagccgggcg
    14401 tacgtaacct ggcgatctcc caggccgttg cgccgcataa tctctattac gcgccggatc
    14461 cttcctcaca aatcgcctgt tcaattggcg gcaatgtggc ggaaaatgcc ggtggcgtcc
    14521 actgcctgaa atatggtctg accgtacata acctgctgaa aattgaagtg caaacgctgg
    14581 acggcgaggc gctgacgctg ggatcggacg cgctggattc acctggtttt gacctgctgg
    14641 cgctgttcac cggatcggaa ggtatgctcg gcgtgaccac cgaagtgacg gtaaaactgc
    14701 tgccgaagcc gcccgtggcg cgggttctgt tagccagctt tgactcggta gaaaaagccg
    14761 gacttgcggt tggtgacatc atcgccaatg gcattatccc cggcgggctg gagatgatgg
    14821 ataacctgtc gatccgcgcg gcggaagatt ttattcatgc cggttatccc gtcgacgccg
    14881 aagcgatttt gttatgcgaa ctggacggcg tggagtctga cgtacaggaa gactgcgagc
    14941 gggttaacga catcttgttg aaagcgggcg cgactgacgt ccgtttggca caggacgaag
    15001 cagagcgcgt acgtttctgg gccggtcgca aaaatgcgtt cccggcggta ggacgtatct
    15061 ccccggatta ctactgcatg gatggcacca tcccgcgtcg cgccctgcct ggcgtactgg
    15121 aaggcattgc ccgtttatcg cagcaatatg atttacgtgt tgccaacgtc tttcatgccg
    15181 gagatggcaa catgcacccg ttaatccttt tcgatgccaa cgaacctggt gaatttgccc
    15241 gcgcggaaga gctgggcggg aagatcctcg aactctgcgt tgacgttggc ggcagcatca
    15301 gtggggaaca tggcatcggg cgagaaaaaa tcaatcaaat gtgcgcccag ttcaacagcg
    15361 atgaaatcac gaccttccat gcggtcaagg cggcgtttga ccccgatggt ttgctgaacc
    15421 ctgggaaaaa cattcccacg ctacaccgct gtgctgaatt tggtgccatg catgtgcatc
    15481 acggtcattt accttttcct gaactggagc gcttctgatg ctacgcgagt gtgattacag
    15541 tcaggcgctg ctggagcagg tgaatcaggc aattagcgat aaaatgccgc tggtgattca
    15601 gggcagcaat agcaaagcct ttttaggtcg cccagtcacc gggcaaacgc tggatgtgcg
    15661 ttgtcatcgc ggcattgtta attacgaccc gaccgagctg gtgataaccg ctcgtgccgg
    15721 aacgccgctg gtggcgattg aagcggcgct ggagagcgcc gggcaaatgc ttccctgcga
    15781 gccgccgcat tatggtgaag aagccacctg gggcgggatg gtcgcctgcg ggctggcggg
    15841 gccgcgtcgc ccgtggagcg gttcggtccg cgattttgtc ctcggcacgc gcatcattac
    15901 cggcactgga aaacatctgc gttttggtgg cgaagtgatg aaaaacgttg ccggatacga
    15961 tctctcacgg ttaatggccg gaagctacgg ttgtcttggc gtgctcactg aaatctcaat
    16021 gaaagtgtta ccgcgaccgc gcgcctctct gagcctgcgt cgggaaatca gcctgcaaga
    16081 agccatgagt gaaatcgccg agtggcaact ccagccatta cccattagtg gcttatgtta
    16141 cttcgacaat gcgttgtgga tccgccttga gggcggcgaa ggatcggtaa aagcagcgcg
    16201 tgaactgctg ggtggcgaag aggttgcggg tcagttctgg cagcaattgc gtgaacaaca
    16261 actgccgttc ttctcgttac taggtacctt atggcgcatt tcattaccca gcgatgcgcc
    16321 gatgatggat ttacccggcg agcaactgat cgactggggc ggggcgttac gctggctgaa
    16381 atcgacagcc gatgacaatc aaatccatcg catcgcccgc aacgctggcg gtcatgcgac
    16441 ccgctttagt gccggagatg gtggctttgc cccgctatcg gctcctttat tccgctatca
    16501 ccagcagctt aaacagcagc tcgacccttg cggcgtgttt aaccccggtc gcatgtacgc
    16561 ggaactttga ggagcaggct atgcaaaccc gattaaccga agagatgcgg cagaacgcgc
    16621 gcgcgctgga agccgacagc atcctgcgcg cctgtgttca ctgcggattt tgtaccgcaa
    16681 cctgcccaac ctatcagctt ctgggcgatg aactggacgg gccgcgcggg cgcatctatc
    16741 tgattaaaca ggtgctggaa ggcaacgaag tcacgtttaa aacacaggag catctcgatc
    16801 gctgcctcac ttgccgcaat tgtgaaacca cctgtccttc tggtgtgcgc tatcacaatt
    16861 tgctggatat cgggcgtgat attgtcgagc agaaagtgaa acgcccactg ccggagcgaa
    16921 tgctgcgcga aggattgcgc caggtagtgc cgcgtccggc ggtcttccgt gcgctgacgc
    16981 aggtagggct ggtgctgcga ccgtttttac cggaacaggt cagagcaaaa ctgcctgctg
    17041 aaacggtgaa agctaaaccg cgtccgccgc tgcgccataa gcgtcgggtt ttaatgctgg
    17101 aaggctgcgc ccagcctacg ctttcgccca acaccaacgc ggcaactgcg cgagtgctgg
    17161 atcgtctggg gatcagcgtc atgtcagcta acgaagcagg ctgttgtggc gcggtggact
    17221 atcatcttaa tgcgcaggag aaagggttgg cacgggcgcg caataatatt gatgcctggt
    17281 ggcccgcgat tgaagcaggt gccgaggcaa ttttgcaaac cgccagcggt tgcggcgcgt
    17341 tcgtcaaaga gtatgggcag atgctgaaaa acgatgcgtt atatgccgat aaagcgcgtc
    17401 aggtcagtga actggcggtc gatttagtcg aacttctgcg cgaggaaccg ctggaaaaac
    17461 tggcaattcg cggcgataaa aaactggcct tccactgccc gtgtacccta caacatgcgc
    17521 aaaagctgaa cggcgaagtg gaaaaagtgt tacttcgtct tggatttacc ttaacggacg
    17581 ttcccgacag ccatctgtgc tgcggttcag cgggaacata tgcgttaacg catcccgatc
    17641 tggcacgcca gctgcgggat aacaaaatga atgcgctgga aagcggcaaa ccggaaatga
    17701 tcgtcaccgc caacattggt tgccagacgc atctggcgag cgccggtcgt acctctgtgc
    17761 gtcactggat tgaaattgta gaacaagccc ttgaaaagga ataaaaaaat gaaaactaaa
    17821 gtcattctta gccagcaaat ggcgagtgca attattgccg caggtcagga agaggcgcag
    17881 aaaaataact ggtctgtttc cattgctgtt gccgatgacg gcggtcatct gctggcgtta
    17941 agtcgcatgg acgattgcgc gccgattgcg gcttacatct cccaggagaa agcgcgtacc
    18001 gccgcgctgg ggcgtcgtga aactaagggc tatgaagaga tggtgaacaa cggacgtacc
    18061 gcgttcgtga ctgcgccgtt actgacgtcg ctggaaggcg gcgtaccggt tgttgttgat
    18121 gggcaaatta ttggtgccgt gggcgtttct ggtttaaccg gagcacagga tgcgcaggtc
    18181 gcgaaagcgg cagcagcggt gttggcgaaa taagcgaaaa cgaggagaga aacaatgagt
    18241 caaaccataa cccagggccg tttacgcatt gacgccaatt ttaaacgttt tgtggatgga
    18301 gaagttttgc cgggcgtgga actggacgct gctgcattct ggcacaatgt tgatgagatc
    18361 gttcacgatc tggcaccaga aaatcgtcag ttgctggcag aacgcgatcg cattcaggcg
    18421 gcgcttgatg agtggcatcg cagcaatccg gggccggtaa aagataaagc ggcctataaa
    18481 tctttccttc gtgaactggg ctacctggtg ccgcaaccgg agcgcgtgac ggtggaaacc
    18541 acgggcattg acagcgaaat caccagtcag gcggggccac agctggtggt tccggcaatg
    18601 aacgcccgct acgcgctgaa cgcggcgaac gctcgctggg gctcactgta cgatgcgtta
    18661 tacggcagcg acatcatccc gcaggaaggg gcgatggtca gtggctacga tccgcagcgc
    18721 ggtgagcagg ttatcgcctg ggttcgacgt ttcctcgatg aatcactacc gctggaaaac
    18781 ggcagctatc aggatgtggt ggcgtttaag gtggtcgata aacaattacg catccagttg
    18841 aaaaatggta aagaaaccac gttacgtact ccagcacagt ttgtcggtta ccgtggcgat
    18901 gccgctgcgc cgacctgcat tttgctgaaa aataacggcc tgcatattga actgcaaatt
    18961 gatgccaacg ggcggattgg caaagacgat ccggcgcaca tcaacgatgt tatcgtcgaa
    19021 gctgctatca gtaccattct cgactgcgaa gattcggtcg cggcggttga tgcggaagat
    19081 aaaatcctgc tgtaccgcaa tctgctgggc ctgatgcagg ggactctgca agagaaaatg
    19141 gagaaaaacg gtcggcaaat cgtacgtaaa ctgaatgacg atcgtcagta caccgccgcc
    19201 gatggctctg aaatttctct gcacggacgc tcgttgctgt ttatccgcaa cgtgggtcat
    19261 ttgatgacca ttcctgtgat ttgggacagc gaaggcaatg aaatcccgga aggtattctt
    19321 gatggcgtca tgactggcgc gattgccctc tatgatttaa aagtgcagaa aaactcgcgc
    19381 actggcagcg tctatattgt gaaaccgaaa atgcacggtc cgcaggaagt ggcgttcgcc
    19441 aacaaactgt ttacccgcat tgagacaatg ctcggtatgg caccgaatac cctgaaaatg
    19501 ggcattatgg atgaagaacg ccggacctcg ctgaacttgc gtagctgtat cgctcaggcg
    19561 cgcaaccgcg tggcgttcat caataccggt ttcctcgacc gtaccggcga tgaaatgcat
    19621 tcggtgatgg aagctggccc gatgctgcgt aaaaaccaga tgaaatcgac gccgtggatc
    19681 aaagcctacg aacgtaataa cgtgctttcc ggtctgttct gtgggctgcg cggtaaagcg
    19741 caaatcggta aaggcatgtg ggcaatgccg gacctgatgg cagacatgta cagccagaag
    19801 ggcgaccaac tgcgtgccgg ggcaaacaca gcctgggttc cgtcaccaac cgctgctacg
    19861 ctccatgcgc tgcactacca ccaaaccaac gtacagagcg tgcaagccaa cattgcccag
    19921 accgagttca atgctgaatt tgaaccgctg ctggacgatc tgctgactat tccggtggct
    19981 gaaaacgcta actggtcggc gcaagagatc caacaagagc tggataacaa cgtgcagggg
    20041 attctggggt acgtggtgcg ttgggtggag caggggattg gttgttcaaa agtgccggat
    20101 attcacaacg tggcgctgat ggaagaccgc gcaacgctgc gtatctccag ccagcatatc
    20161 gccaactggt tacgtcacgg tattctgacc aaagaacagg tgcaggcgtc gctggagaat
    20221 atggcgaaag tggttgatca gcaaaacgct ggcgatccgg cttatcgtcc gatggcgggg
    20281 aatttcgcta actcgtgtgc ttttaaagct gccagcgatt taatcttcct cggcgtgaaa
    20341 cagccaaacg gttataccga accgttatta cacgcctggc gtttacgcga aaaagaaagt
    20401 cattaatagc gtcaaaaaag ccccggcaag ttttattgtc ggggctatga tagtaactgc
    20461 ggtagtacat ttatcccgga aataaccgga gcgattatca gggtttaaca atattaaata
    20521 cccgtagc
//
LOCUS       DADLSA010000065        20521 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.065, whole genome shotgun sequence.
ACCESSION   DADLSA010000065 DADLSA010000000
VERSION     DADLSA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.065"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="JN691_002337"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="JN691_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAY7243763.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="JN691_002338"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="JN691_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HAY7243764.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="JN691_002339"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="JN691_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAY7243765.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JN691_002340"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JN691_002340"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAY7243766.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JN691_002341"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JN691_002341"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAY7243767.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGREPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="JN691_002342"
     CDS             4449..4814
                     /locus_tag="JN691_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAY7243768.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4964..5482
                     /locus_tag="JN691_002343"
     CDS             4964..5482
                     /locus_tag="JN691_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4154 domain-containing protein"
                     /protein_id="HAY7243769.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="JN691_002344"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="JN691_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="JN691_002345"
     CDS             6713..7195
                     /locus_tag="JN691_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAY7243770.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JN691_002346"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JN691_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAY7243771.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JN691_002347"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JN691_002347"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAY7243772.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JN691_002348"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JN691_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAY7243773.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JN691_002349"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JN691_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAY7243774.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JN691_002350"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JN691_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAY7243775.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10864..11730
                     /locus_tag="JN691_002351"
     CDS             10864..11730
                     /locus_tag="JN691_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAY7243776.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLVIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="JN691_002352"
     CDS             11751..13037
                     /locus_tag="JN691_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY7243777.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JN691_002353"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JN691_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAY7243778.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="JN691_002354"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="JN691_002354"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAY7243779.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="JN691_002355"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="JN691_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAY7243780.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="JN691_002356"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="JN691_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAY7243781.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="JN691_002357"
     CDS             complement(16985..17275)
                     /locus_tag="JN691_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAY7243782.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JN691_002358"
     CDS             complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JN691_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAY7243783.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGG
                     WKFTPLSQEACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="JN691_002359"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="JN691_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAY7243784.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="JN691_002360"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="JN691_002360"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="JN691_002361"
     CDS             19137..20378
                     /locus_tag="JN691_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7243785.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKKRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5298 a         5357 c         4820 g         5046 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcattaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgctaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacaggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggctcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaagcaatt tggtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acaccccctt tgaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaggggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcaaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aggagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gccttatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cacccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgata tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca ttcccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgtcttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcgacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccaa ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctggtgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtgccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg cgagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttga ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgcta
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtactgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacccggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gagtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgccccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagaaacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DADLSA010000066        20449 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.066, whole genome shotgun sequence.
ACCESSION   DADLSA010000066 DADLSA010000000
VERSION     DADLSA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.066"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(234..929)
                     /gene="aqpZ"
                     /locus_tag="JN691_002362"
     CDS             complement(234..929)
                     /gene="aqpZ"
                     /locus_tag="JN691_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HAY7243786.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLINIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            1354..3012
                     /locus_tag="JN691_002363"
     CDS             1354..3012
                     /locus_tag="JN691_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAY7243787.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(3009..3965)
                     /locus_tag="JN691_002364"
     CDS             complement(3009..3965)
                     /locus_tag="JN691_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAY7243788.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            4116..5231
                     /gene="macA"
                     /locus_tag="JN691_002365"
     CDS             4116..5231
                     /gene="macA"
                     /locus_tag="JN691_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAY7243789.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5228..7175
                     /gene="macB"
                     /locus_tag="JN691_002366"
                     /pseudo
     CDS             5228..7175
                     /gene="macB"
                     /locus_tag="JN691_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
     gene            complement(7248..7472)
                     /gene="cspD"
                     /locus_tag="JN691_002367"
     CDS             complement(7248..7472)
                     /gene="cspD"
                     /locus_tag="JN691_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAY7243790.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7795..8115
                     /gene="clpS"
                     /locus_tag="JN691_002368"
     CDS             7795..8115
                     /gene="clpS"
                     /locus_tag="JN691_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAY7243791.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8146..10422
                     /gene="clpA"
                     /locus_tag="JN691_002369"
     CDS             8146..10422
                     /gene="clpA"
                     /locus_tag="JN691_002369"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAY7243792.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTAALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10825..10912)
                     /locus_tag="JN691_002370"
     tRNA            complement(10825..10912)
                     /locus_tag="JN691_002370"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10876..10878),aa:Ser,seq:gga)
     gene            complement(11166..11384)
                     /gene="infA"
                     /locus_tag="JN691_002371"
     CDS             complement(11166..11384)
                     /gene="infA"
                     /locus_tag="JN691_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAY7243793.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11669..12373)
                     /gene="aat"
                     /locus_tag="JN691_002372"
     CDS             complement(11669..12373)
                     /gene="aat"
                     /locus_tag="JN691_002372"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAY7243794.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12415..14136)
                     /gene="cydC"
                     /locus_tag="JN691_002373"
     CDS             complement(12415..14136)
                     /gene="cydC"
                     /locus_tag="JN691_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAY7243795.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(14137..15903)
                     /gene="cydD"
                     /locus_tag="JN691_002374"
     CDS             complement(14137..15903)
                     /gene="cydD"
                     /locus_tag="JN691_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAY7243796.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(16026..16991)
                     /gene="trxB"
                     /locus_tag="JN691_002375"
     CDS             complement(16026..16991)
                     /gene="trxB"
                     /locus_tag="JN691_002375"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAY7243797.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17536..18030
                     /gene="lrp"
                     /locus_tag="JN691_002376"
     CDS             17536..18030
                     /gene="lrp"
                     /locus_tag="JN691_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAY7243798.1"
                     /translation="MVDSKKRSGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            18165..>20449
                     /gene="ftsK"
                     /locus_tag="JN691_002377"
     CDS             18165..>20449
                     /gene="ftsK"
                     /locus_tag="JN691_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY7243799.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIALFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAP"
BASE COUNT         5171 a         5093 c         5437 g         4748 t
ORIGIN      
        1 gcacccccgg gctctggcga acaaaatcgc cgctgcgcgg taccttcagc ttatcccctt
       61 cggcaaccgg gtcgggcgcg aggtaacgtc ctgtaagacg cgccctcagc ccacatccat
      121 gtgggctgcc ccggccttcg gggaacgctt cagcgatttt gacgccacca atacccgtgc
      181 ggtttgtaat gtcgaagata aaacaaccag ggcagataaa atgccaatgc taattaatca
      241 cgcttttcca gcagagtccg gtaaatcaga ccaccgataa tgccgccgac aattggcacc
      301 acccagaaga accacagttg ttctaatgcc cagccgccct ggaagatagc aaccgcggtg
      361 ctgcgcgccg ggttaacaga agtgttagtc accggaatat tgattaagtg aatcagggtt
      421 aaggccagac caatagcgat cggcgcaaaa cctgccggag cgaatttgtc ggttgcaccg
      481 tggatcacta acaggaagcc cgcactcaat accagttcaa ctaccagcgc ggaaagcatg
      541 gaataaccgc ctggtgaatg ctcgccataa ccgttagaag caaaaccgct ggctgccgcg
      601 tcaaaacccg ttttaccact ggcaattaaa tacagcagcg ccgctgcaac aataccgccg
      661 acaacctggg caattacgta gccaacgact tcttttgccg gaaaacgtcc gccagcccat
      721 aaaccaatag tgaccgccgg gttaaaatga ccaccagaaa tatgaccaac agcgaaggcc
      781 atcgtcagaa cggtcaaacc aaatgccagc gccacgccgg caaaacctat gcctaattcc
      841 gggaagcctg cggccagtac agcactacca cagccaccaa aaacaagcca gaaagtacca
      901 aaacattcag ctgctaattt tctgaacata tccacctcaa ttaaaaattg accctgtgaa
      961 aaatatggtc gttttatagg gtcgtcgtaa aaaatgacga cggaaataat gcgcggctat
     1021 tttaaaaacg aaggcgagtc attcaccaga taaataaatc cagtaaattt gatttagggc
     1081 aacagcgggt tgccccatat agtcatttgt ctgattgaca gtgtagtgca agcaaaagat
     1141 ttaatccttt aggcgtaata aaaaataatt tatcatgcta attatttgat tttgttgttt
     1201 ttgcgggctt atcagcaaga gggagtataa cgcgattatt cgctcatttt tcagacaatt
     1261 gccatgctta aatgtgatgt catcacgtat tagcaaggcc tttcccgtta tactgccagc
     1321 gtaaaggata agtcacatat ttctggaggg gatatgattc ttgagcgcgt tgaaattgtg
     1381 ggttttcgcg gtatcaaccg tttgtcgttg atgctcgaac aaaacaacgt cctgattggg
     1441 gagaacgcgt ggggtaaatc cagcttgctg gacgccttaa ccctgctgct atcgccagaa
     1501 tcagatctct accattttga gcgcgacgat ttctggttcc cgccgggaga tatcaacggg
     1561 cgagaacatc atctgcatat tattttgacc ttccgcgaat cgctgccagg ccgacatcgg
     1621 gttcgccgtt atcggccgct ggaagcgtgc tggacgccat gcaccgatgg ctatcaccgt
     1681 attttttatc gtctggaagg ggagagtgcg gaagacggca gcgtgatgac actgcgcagt
     1741 tttctcgata aagacggaca tccgattgat gttgaggata ttaacgatca ggcacgccat
     1801 ctggtgcgtt taatgccggt gctgcgcttg cgtgatgccc gttttatgcg ccgtattcgt
     1861 aacggcacgg tgccaaatgt ccccaatgtg gaagtcaccg cgcgccagct cgatttcctc
     1921 gcccgtgagt tgtcctcaca tccgcaaaat ctctctgatg gacagattcg tcagggactt
     1981 tccgcaatgg tgcagttgct tgagcattat ttctctgagc agggggctgg acaggcgcga
     2041 tatcgtttaa tgcggcggcg ggccagcaat gagcaacgaa gctggcgcta tctggacatc
     2101 atcaaccgga tgattgaccg acctggaggg cgctcgtatc gggttatttt gctcggcctg
     2161 tttgctactt tattgcaggc aaaaggcaca ttgcgactgg ataaagacgc gcgtccgtta
     2221 ttgctaattg aagatccaga aacccgcctg catccgatta tgctttccgt tgcctggcat
     2281 ctgttgaatc ttttgccttt acagcgtatt gctaccacca actctggcga gctgctttcg
     2341 ttaactccag tggaacatgt ttgccgcctg gtgcgtgaat cttctcgtgt tgccgcctgg
     2401 cgtctggggc caagtggctt gagcaccgaa gacagccgcc gcatctcttt tcacattcgt
     2461 tttaatcgtc cgtcatcgct gtttgctcgc tgctggttgc tggtggaagg ggaaacggaa
     2521 acctgggtta tcaatgaact ggcgcgtcag tgcggacatc attttgatgc cgaagggatc
     2581 aaggtcattg agtttgccca gtccgggcta aagccactgg ttaaatttgc ccgccgaatg
     2641 gggattgaat ggcatgtact ggtcgatggc gatgaagcag ggaagaaata tgccgctacg
     2701 gtacgtagcc tgttgaataa cgatcgggaa gccgaacgag aacatttaac ggcgttaccg
     2761 gcgctggata tggaacattt tatgtatcgc cagggatttt ccgatgtgtt ccaccgcgtg
     2821 gcgcaaatcc cggaaaatgt accgatgaat ctacgcaaaa ttatctcgaa agcgatccat
     2881 cgctcttcca aacccgatct tgccattgaa gtggcaatgg aggcaggacg tcgtggtgtg
     2941 gactccgtac caacgctgct gaaaaaaatg ttctcacgcg tgctgtggct ggcgcgcggt
     3001 cgcgcggatt aaccgcgaaa catcgtggcc atttgtggct gaatagcgtc gagcatctca
     3061 tagcgccgac ggtattcagc ccgtttttta ctggcgattt cggcaatctc ttttcgtgct
     3121 atctgtgctg gaaggcggta atggcgttca gcatcacata cgccgccaac cgactcccag
     3181 aacgcgttgt aatcagcatg gatcttgcct tctttatcgc gataacgcag gctgcggtaa
     3241 atatgcgttt cattgctgac ggcaataatc tgctctacct gcaaacgttg ggcaaacaga
     3301 caggccgctt ccatcacgag gcgtttggga aatagcccgt ggcaggcttt cgtcgcattc
     3361 tggatttcct gatgtggaat ttcccatttt gcgccttgca gtccgccaat aaacatcgtt
     3421 cttttcccct gatattcaca cagggtaaac gtgatctctg ccagaggaat accttcgctg
     3481 ttgcggaaca ggattgtgct gtcaccttct ttatccattg agatcatcat ggtcagctca
     3541 agcgtgaact gctcaccgtt tttgccttcc agtttagcca gttgcagccc gggggtattc
     3601 aaatataagc tgaattcttc cgccgacata cacccgcgga gtaacgcata atggtaacgt
     3661 aacgcctcca gcaattgctt acggctaaga ttcgccgcaa ggtaagggcg atgcagacgc
     3721 acaggcagtc gcggctggcg cgttaacaac acattgagat taggccagtg ggaaagttcg
     3781 ttcatccact caacgcttaa acgcggcata atcaacgagc gcagcaaaaa tttctggcga
     3841 aaactacagc gatgccagaa tttacccggc cgacactgtc cacgtgccag actaagaaaa
     3901 agtgacaggc tgctgagaga ttcagatggc gtaaaggtcc gttcagttag ctgcgacata
     3961 ttcatgaaat caatggttat acatgacgtc gatttcacca ttgcgtatct taaccaaaca
     4021 tcaatagtgt gattactaac gtaaatttta gggttttgtt gatatttcgt tgaagttaat
     4081 gacccggatt ggcatatgga gtattcagaa tatttatgaa aaagcggaaa accgtgaaga
     4141 agcgttacgt tattgcgctg gtgatagtca tcgccggact gattacgtta tggagaattc
     4201 ttaacgcacc cgtgccgact tatcagacac tgattgtgcg ccccggtgat ttacagcaaa
     4261 gcgtgctggc gaccggaaag ctggacgcgc tgcgtaaggt tgacgtgggc gcgcaggtca
     4321 gcggtcagtt gaaaacgctg tcggtggcga ttggcgataa agtaaaaaaa gaccagcttt
     4381 taggggtcat tgatcctgaa caggctgaaa accagatcaa ggaggtcgaa gcaacgctga
     4441 tggagctacg cgcgcagcgg cagcaggcgg aagcggagct gaaactggcg cgggtgacgt
     4501 attcccgtca gcaacgtctg gcacaaacgc aagcggtttc actgcaggat ctcgataccg
     4561 ccgcgacgga gatggctgtg aaacaggcgc aaattggcac cattgacgcg caaatcaagc
     4621 gcaatcaggc ctctctcgat acggctaaaa ccaatctcga ttacacccgt atcgttgccc
     4681 cgatggccgg ggaagtgacg caaatcacca ctctgcaagg ccagacggtg attgccgcac
     4741 agcaagcacc gaacattctg acgctggcag atatgagcac tatgctggta aaagcgcagg
     4801 tttctgaagc ggatgtaatc cacctgaagc cggggcaaaa agcctggttt acggtgcttg
     4861 gcgatccact gacgcgctac gaggggcaaa tcaaggatgt gctaccgacg ccggaaaagg
     4921 taaacgacgc tattttctat tacgcccgtt ttgaagtacc caaccccaat ggtttgctgc
     4981 ggctggatat gaccgcgcaa gtgcatattc agctcaccga tgtgaaaaat gtgttgacga
     5041 tccctctgtc ggcgttaggc gatccggttg gcgataatcg ttataaagtc aaattgttgc
     5101 gtaatggtga aacacgcgag cgtgaagtga cgattggcgc acgtaacgat accgatgttg
     5161 agattgtcaa agggctggaa gcgggcgatg aagtggtgat tggtgaggcc aaaccaggag
     5221 ctgcacaatg acgcctttgc tcgaattaaa ggatattcgt cgcagctatc ctgccggtga
     5281 tgagcaggtt gaggtgctga agggcatcag cctcaatatt tatgcgggtg agatggtcgc
     5341 gattgttggc gcttcgggtt ccggtaaatc gaccctgatg aatattctcg gctgcctgga
     5401 taaggccacc agcggcacct atcgcgtagc cggtcaggat attgccacgc tggacgccga
     5461 tgcgctggcg caactgcgcc gcgagcattt cggctttatt ttccagcgtt accatttgct
     5521 ttcgcattta accgccgagc agaacgttga agtacccgcc gtctatgccg gtcttgagcg
     5581 gaaacagcga ctgcttcgtg cccaggagtt gctgcaacgg ctggggctgg aagaccgtac
     5641 agagtattat ccggcacagc tttcgggtgg tcagcaacag cgcgtcagca tcgcgcgggc
     5701 attgatgaac ggcggtcagg taattcttgc cgatgaaccg accggcgcac tggacagcca
     5761 ttctggcgaa gaggtgatgg cgatcctgca tcagctgcgc gatcccgggc atacggtgat
     5821 tatcgtcacc cacgatccgc aggtcgctgc tcaggccgag cgggtgatcg aaattcgcga
     5881 cggcgaaatt gtgcgcaatc ctcccgccat tgaaaaagtg aatgttgctg gcgggacgga
     5941 gcctgttgtc aatacagtgt ctggctggcg gcagtttgtc agcggtttta acgaggcgct
     6001 gacgatggca tggcggacgc tggcagcgaa taaaatgcgt actttactga ccatgctggg
     6061 gattattatc ggtattgcgt cggtggtttc cattgtcgtg gtgggtgacg ccgccaaaca
     6121 aatggtgctg gcggatattc gttctattgg tacgaatact attgatgtct atcccgggaa
     6181 agattttggc gatgacgatc cgcaatatca gcaggcgctg aagtacgacg acttaatcgc
     6241 catccaaaaa caaccgtggg tcgcctcagc cacacctgcc gtctcgcaaa acctgcgcct
     6301 gcgttataac aatgttgatg ttgctgccag tgccaatggc gtgagcggcg attattttaa
     6361 tgtctatggc atgacacttc agtgaaggaa acacctttaa tcaggagcag ctgaacggtc
     6421 gtgcgcaggt cgtggttctc gacagaaata ctcgccgcca gcttttcccc cataaagcag
     6481 atgtggttgg cgaggtgatt ctggtcggca atatgcccgc cagagtcatt ggtgtggcgg
     6541 aagaaaaaca gtcgatgttt ggtagcagta aagtgctgcg tgtctggcta ccttacagca
     6601 cgatgtccgg gcgagttatg ggccagtcgt ggcttaactc cattactgtc agggtgaaag
     6661 aaggatttga cagcgccgag gcggaacagc aactcacgcg tttactttca ctgcgccacg
     6721 gtaagaagga tttctttacc tggaacatgg acggcgtctt gaaaactgtt gaaaagacca
     6781 cacgtacttt acaactgttt ctgacgctgg tggcggtgat ttcgctggtg gtgggcggta
     6841 ttggtgtaat gaatattatg ctggtgtcag tgaccgagcg gacgcgggaa attggcattc
     6901 gcatggctgt aggtgcgcga gcaagcgatg ttttgcaaca gttcctgatc gaagccgtac
     6961 tggtttgcct ggtcggtggc gcgttgggaa taacactgtc actgttaatt gctttcacct
     7021 tgcagctttt cttacccggc tgggagattg gtttttcacc gttggcgctg ctgctggcgt
     7081 ttctctgctc gacggccacc gggattttat ttggctggtt acccgcacga aatgcggcac
     7141 gactggatcc agtagatgct ctggcacgag agtaattttt gagataaaaa tgccagccga
     7201 tcgggctggc attttgcctt taggatgtac acaatgagac agaagagcta tgcgactgcc
     7261 gcttctactt cgacgggcac aataacactg gcgtgattgc cttttggccc ctggtggaca
     7321 tcaaactgaa cggattgtcc agcttttagc gttctgtaac catccatctg aatggtggaa
     7381 taatgagcga aaatatcttc gccgccgcct tcagggcaga tgaaaccaaa ccctttggca
     7441 ttgttgaacc acttaacagt acccttttcc atgcttcgac atccttcgca aatcttatac
     7501 aagtaagatg gaataaaccg gggtcagaga gggggctgtt caaaacctcg ccaactctag
     7561 aaatacaatt tagagaatta gggcgagccg tcaagcattt gacaggggcc agggggcagg
     7621 tatgaatcaa aaatttgaag cagttaacgc tattgacagg aatgtgacag atgtcgctga
     7681 tgccaacgat agatgatagt tatctatcat gtggagtaga ttggtcaggc aaataagctc
     7741 ttgtcagcgg cagggcgttc tgccgataac cgtaaccgaa gatgataact gacaatgggt
     7801 aaaacgaacg actggctgga ctttgatcaa ctggcggaag aaaaagttcg cgacgcgcta
     7861 aaaccgccat ctatgtataa agtgatatta gtcaatgatg attacactcc gatggagttt
     7921 gttattgacg tgttacaaaa attcttttct tatgatgtag aacgtgcaac gcaattgatg
     7981 ctcgctgttc actaccaggg gaaggccatt tgcggagtct ttaccgccga ggttgcagaa
     8041 accaaagtgg cgatggtgaa caagtacgcg agggagaatg agcatccatt gctgtgtacg
     8101 ctagaaaaag cctgaatgca ggcataaaaa ttgggggagg tgcctatgct caatcaagaa
     8161 ctggaactca gtttaaatat ggctttcgcc agagcgcgcg agcaccgtca tgagtttatg
     8221 accgtcgagc acttgttact ggcgctgctc agtaacccat ctgcccggga ggcgctggaa
     8281 gcgtgttctg tggatttggt tgcgctccgt caggaactgg aagcctttat tgaacaaacc
     8341 acacccgttc tgcctgccag tgaagaggag cgcgacacac agccgacgct gagttttcag
     8401 cgtgtactgc aacgtgcggt cttccatgtc cagtcctccg gtcgcaatga ggttaccggt
     8461 gcaaacgttc tggtcgctat cttcagcgaa caggagtcgc aggcggcata tctgttgcgt
     8521 aaacatgaag tcagccgtct cgatgtggtg aactttatct ctcatggcac gcgtaaagac
     8581 gagccgacac agtcttctga tcctggcagc cagccaaaca gcgaagaaca agctggtggg
     8641 gaggaacgta tggagaattt cacgacgaac ctgaaccagc ttgcgcgtgt gggcggaatc
     8701 gacccactaa ttggtcgtga gaaagagctg gagcgtgcta ttcaggttct ctgccgtcgc
     8761 cgtaaaaaca acccgctgct ggtgggggaa tctggtgtcg gtaaaaccgc gattgcagaa
     8821 ggtcttgcct ggcgaattgt tcagggtgat gtgccggaag tgatggctga ctgtacgatt
     8881 tactctctcg atatcggttc tctgttagcg ggcacaaaat atcgcggcga ctttgaaaaa
     8941 cgttttaaag cgttgctcaa gcagctggag caggacacta acagcatcct gtttattgat
     9001 gagatccaca ccattatcgg tgcgggtgca gcgtctggtg gtcaggtcga tgcggctaac
     9061 ctaatcaaac cgttgctctc cagcggtaaa attcgcgtaa ttggttcgac aacctatcag
     9121 gagttcagca acattttcga gaaagaccgt gctctggcgc gtcgcttcca gaaaattgat
     9181 attactgaac cgtcgatcga agaaactgtt caaatcatca atggcctgaa accgaagtat
     9241 gaagcgcacc acgacgtgcg ttataccgca aaagcggtgc gtgcggcggt agagctggcg
     9301 gtgaaataca ttaacgatcg tcatctgccg gataaagcca ttgatgttat cgacgaagcg
     9361 ggcgctcgcg cacgtctgat gccggtaagc aaacgcaaga aaaccgttaa tgtggcggat
     9421 attgagtccg tggtggcccg tattgcacgc attccagaga agagtgtttc tcagagtgac
     9481 cgcgataccc tgaaaaacct cggcgatcgc ctgaaaatgc tggtcttcgg ccaggataaa
     9541 gccattgagg cgctgactga agccattaag atggcgcgtg caggtttagg tcacgaacat
     9601 aaaccggtcg gttcgttcct gtttgccggt cctaccgggg tcgggaaaac agaggtgacg
     9661 gtacagcttt cgaaagcgtt gggcattgag cttctgcgct ttgatatgtc cgagtatatg
     9721 gaacgccata ccgtcagccg tctgattggt gcgcctccgg gatacgttgg ttttgatcag
     9781 ggcggtttgc tgaccgatgc ggtaatcaag catccacatg cggtgctgct gctggacgaa
     9841 atcgagaaag cgcatccgga cgtgttcaat attctgttgc aggtgatgga caacggtacg
     9901 ctgaccgata acaacggacg caaagcggac ttccgtaacg tggtgctggt gatgaccact
     9961 aacgccgggg tacgggaaac tgagcgcaaa tccattggtc ttatccacca ggataacagc
    10021 accgatgcga tggaagagat caagaagatc tttacaccgg aattccgtaa ccgtctcgac
    10081 aacattatct ggttcgatca tctgtcaact gacgtgatcc atcaggtggt ggataaattc
    10141 atcgtcgagt tgcaggttca gctggatcag aaaggtgttt ctctggaagt gagccaggaa
    10201 gcgcgtaact ggctggccga gaaaggttac gaccgggcaa tgggcgcacg tccgatggcg
    10261 cgtgtcatcc aggacaacct gaaaaaaccg ctcgccaacg aactgctgtt tggttcgctg
    10321 gtggacggcg gtcaggtgac ggctgcgctg gataaagaga aaaatgagct gacttacgga
    10381 ttccagagtg cacaaaagca caaggcggaa gcagcgcatt aatctgattg tcaggtaggt
    10441 tggtgaagtc cgtaatctcg tcaggggtta cggacttttt atttatgggg tggaacggtg
    10501 aaaaccctat ttttggaggt gaaggtaagt tgttgataat tagtgctgct ggaaggtaag
    10561 gataaaaaag ggtgctgcag gagaatggga tggttttgct ttattaacaa cgggctaaac
    10621 gtgtagtatt tgagttcact gccgtacagg cagcttagaa atcgacgggg tgcggtaaaa
    10681 cctttgcgaa cgcgttcact gccgtacagg cagcttagaa attcacaggt aacatactcc
    10741 acccgcccac catgttcact gccgtacaga cagataaaat gcgaaaaaaa agctcgcact
    10801 ttcgtacgag ctcttcttta aatatggcgg tgaggggggg attcgaaccc ccgatacgtt
    10861 gccgtataca cactttccag gcgtgctcct tcagccactc ggacacctca ccaaattgtt
    10921 ttgctaccaa acctcatggg tggcaacggg gcgttactat agggagttgg agtaaaacgg
    10981 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaact tgtgcagtat
    11041 atctacatcg agacaagtta tggacttata cttccaaagt acttcataca tatcacaaaa
    11101 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
    11161 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
    11221 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
    11281 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
    11341 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
    11401 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
    11461 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
    11521 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
    11581 aaaattcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
    11641 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag caccgtggta
    11701 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataatta aggtaatccc
    11761 ggcggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
    11821 taagcttacc gccatgaccg ataaattcct cacagaatac cagaagcgcc gttttagacg
    11881 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
    11941 acataccgcc gacaagctca tcttcacgcc agacttcaat ggagtgggca tgaccgagtt
    12001 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
    12061 cgctggcaca gccttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
    12121 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattctggc catagcaccg
    12181 cgcggggatc gggcgaccac cagaggatgg ggtcgcctgg agaaaaccac ggaaaaatac
    12241 cacgctggta agccattaac aggcgcgcag ggctaagatc gcccccaagt gccagcaggc
    12301 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
    12361 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
    12421 accctgcttg aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
    12481 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
    12541 tcgatgggtg accattaaca ccgttttctc acgcatcatt tctgcaagca attcaaggat
    12601 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
    12661 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
    12721 tccgccttca cctaaccaac tgttgagacc tgcatcctcg agcagcttct ccagaccaac
    12781 gcgacgcaag atctccgaca gagcctcatc actactgcca ggcgaggcga gtaacagatt
    12841 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
    12901 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct cgccctgttg
    12961 cgggtcccat gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
    13021 gagaatcgct atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
    13081 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
    13141 acgagtttgg gtatcaggga aggtgacctc cggtttttga tccgttaagt cagagatacg
    13201 tacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagtgc
    13261 ttcaaacgcg gctaacgcac aaaagacaaa cagggcaatt aatgcgccgg gttgagcatt
    13321 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
    13381 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
    13441 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
    13501 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt
    13561 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
    13621 cagtaacata acgctgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat
    13681 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
    13741 atgatcgagc gtatcaacat ccgccaccac gcggttgagc aattcgccct gacgatagcg
    13801 cgccagtccg gcaggggaga ggggcaacaa tttgctgaag gtgtaaatgc gcagatgctg
    13861 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
    13921 ggtgattgcg gcaccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
    13981 ggcaaccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
    14041 actggcgagc agcgtcacaa ttgccagcac aataccaaga cttaacatcc atttatgacg
    14101 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
    14161 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
    14221 aatccggcca tcctgcataa cccaaatgac atcccagtca gcaagatctt ctaactggtg
    14281 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
    14341 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
    14401 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
    14461 gtcgccaaca ggtgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc
    14521 gttatccagc gctgcttgta attcttgttc gctggcatca ggtcgcgcca gtagtacgtt
    14581 atcccgcaat tttgctgccg gtaattgtgg gttttgccca acccaggaga gatgtttacg
    14641 ccatgattct ggtgataaat cacgtaattc tatcccgttg attcgtaatg atccctgata
    14701 tgagagaaaa ccagaaagcg cgttcagcag tgagctttta cctgaaccgc tgcggccaac
    14761 caacactgca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc
    14821 ttccggcgac gtgataaaca gatcctcggc ctcaatggtc agcggatcgg tcaatgctaa
    14881 ttccgcctca ccgcgctgcg gatgggcgag cggggtttcc ataaacgttt tcagactgtc
    14941 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
    15001 gaaaaactct ggcgcaagga tcagggccag aaaacctgca gccagcgtca ccccagtatc
    15061 gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
    15121 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaacgcca gtcgtagcac
    15181 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
    15241 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
    15301 taagcgagca agagcgagaa agttacgccg gttagcatcg gcagccccca ttccaaccag
    15361 cgccataaac aacggaatta gcggtgctgt gcccagcaga atgagcgccg cagcccagtt
    15421 agaggggaag atcgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
    15481 cagatagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
    15541 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
    15601 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
    15661 ccagaccacc catgcgcgca atacaaaggt cagaaccagt aacgtaaagg gaagcagcag
    15721 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
    15781 ctgggcaatg atcaatatgc cgcttacaaa gcccagcaga cgagaaatat tcagccaacg
    15841 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gagatttatt
    15901 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
    15961 aaaaataaag gcgacccata gtcgcaaggt gtcgccttct ttacttttgt tactgatttg
    16021 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
    16081 cagccagtac cggccgaagt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg
    16141 gcaaagacgc caggaatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
    16201 atgtagccgt tttccagttc cagctgccct tcgaaaatcg cagtattcgg gctgtgaccg
    16261 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
    16321 cgcagacgaa caccagtgac acccatttga tcgccggtca cttcttccag cgtacggttg
    16381 gtgtgcagaa tgatgttgcc gttctccact ttatccatca gacgcttaat aaggattttt
    16441 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
    16501 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
    16561 tagaagaaac cgtcgcaggt tgcacaagca gaaaccccac ggcctttaaa ggcttcttca
    16621 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
    16681 tattcgccgt tatcgccatt cagacggaac ggacggtttt gcagatccac cttgttgata
    16741 tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg ctccattaat
    16801 aacggaccgg tcagatcgtt tggatcacca ggccagtttt ccacttccgt ggtggtggtc
    16861 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
    16921 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
    16981 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
    17041 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
    17101 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
    17161 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
    17221 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
    17281 acagtattct tatatgcgca taaccatgta tgtaaatacc atgtttaccg tgctagtgaa
    17341 atctacgtat ggcgtggaca gacgccattc gtgatgtcga tagctgccac aaggcaacgg
    17401 tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt
    17461 gacgggtagc gactctgaac agtgatgttt cagggtcaga caggagtagg gaaggaatac
    17521 agagagacaa taataatggt agatagcaag aagcgctctg gcaaagatct cgaccgtatc
    17581 gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct
    17641 aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa
    17701 gggtttattc agggctatac ggcgctgctt aacccccatt atctggatgc atcacttctg
    17761 gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc
    17821 gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatctggtga tttcgactac
    17881 ctgttgaaaa cacgcgtgcc ggatatgtca gcttaccgta agttgctggg ggaaaccctg
    17941 ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag
    18001 agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt
    18061 gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg
    18121 ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca
    18181 ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt
    18241 tgctgatcct tattgccctg tttgccgtct ggttgatggc tgccttacta agctttaacc
    18301 cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga
    18361 tgcccggtgc gtggttggca gatacgctgt tctttatttt tggcgtgatg gcttacacca
    18421 ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcatcagtcc agcgacgaat
    18481 acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct
    18541 cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca
    18601 ttggcagctt actaagcact acgctacaac cactgctaca cagtagcggg ggaactattg
    18661 cgctgctctg cgtttgggca gcgggcctga cgttgttcac cggttggtca tgggtgacta
    18721 ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc
    18781 gtcgcgatga tacctgggtc gatgaagatg agtatgaaga cgacgaagag tatgaagatg
    18841 aaaatcacgg caaacagcat gaatcacgcc gtgcccgtat tcttcgtggc gcgctagcgc
    18901 gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca gacgctgcgt
    18961 tgttctccgg taagcggatg gatgatgacg aagagattac ctacactgca cgcggtgtgg
    19021 ctgccgaccc ggacgacgtc ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg
    19081 aatacgatcc attattaaac ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg
    19141 ctaccacggc gacacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg
    19201 ttgcctctgt tgatgttcca ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc
    19261 cacaaacggg agagccggtt attgctcctg caccggaagg ttacccacag cagtcacaat
    19321 atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca accagtacag ccgcagcagc
    19381 cgtattatgc acctgcagct gaacagcctg cgcaacagcc gtattatgcc cctgcgccag
    19441 aacaaccggt ggcaggtaac gcctggcaag ccgaagagca gcaatccact tttgctccac
    19501 agtctacata ccagactgag caaacttatc agcagccagc cgctcaggag ccgttgtacc
    19561 aacagccgca acccgttgaa cagcagcctg ttgtggagcc tgaacccgtt gtagaagaga
    19621 caaaacccgc gcgtccgccg ctttactatt ttgaagaagt ggaagagaag cgagcccgtg
    19681 aacgtgaaca acttgcggcc tggtatcaac cgattccaga accggttaaa gaaccagaac
    19741 cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcagt acctccagta gaagccgctg
    19801 ccgctgtttc tccgctggca tctggcgtga aaaaagcgac actggcgacg ggggctgccg
    19861 caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg tggaccgcgt ccgcaggtca
    19921 aagaggggat tggcccgcag ttgccacgac cgaaacgtat ccgcgtgcca actcgtcgtg
    19981 aattggcgtc ttacggtatt aagctgccct cacagcgtgc ggcggaagaa aaagcccgtg
    20041 aagcccagcg caatcagtat gattctggcg atcagtataa cgatgatgaa atcgatgcga
    20101 tgcagcagga tgaactggca cgtcagttcg cccagacaca gcagcaacgc tatggcgaac
    20161 agtatcaaca tgatgtgccc gtaaacgcag aagatgcaga tgctgcggca gaggctgaac
    20221 tggctcgtca gtttgcgcaa actcaacaac aacgttattc cggcgaacaa ccggctgggg
    20281 cgaatccgtt ctcgctggat gattttgaat tttcgccaat gaaagcgttg ctggatgatg
    20341 gtccacacga accgctgttt acgccaattg ttgaacctgt acagcagccg caacaaccgg
    20401 ttgcaccgca gcagcagtat cagcagccac aacagccggt tgcgccaca
//
LOCUS       DADLSA010000067        20441 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.067, whole genome shotgun sequence.
ACCESSION   DADLSA010000067 DADLSA010000000
VERSION     DADLSA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.067"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..214)
                     /locus_tag="JN691_002378"
     CDS             complement(<1..214)
                     /locus_tag="JN691_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP endonuclease"
                     /protein_id="HAY7243800.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKE"
     gene            complement(222..932)
                     /locus_tag="JN691_002379"
     CDS             complement(222..932)
                     /locus_tag="JN691_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAY7243801.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1148..2971)
                     /gene="typA"
                     /locus_tag="JN691_002380"
     CDS             complement(1148..2971)
                     /gene="typA"
                     /locus_tag="JN691_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAY7243802.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            3344..4753
                     /gene="glnA"
                     /locus_tag="JN691_002381"
     CDS             3344..4753
                     /gene="glnA"
                     /locus_tag="JN691_002381"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAY7243803.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEAAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            4926..5975
                     /gene="glnL"
                     /locus_tag="JN691_002382"
     CDS             4926..5975
                     /gene="glnL"
                     /locus_tag="JN691_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HAY7243804.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            5987..7396
                     /gene="glnG"
                     /locus_tag="JN691_002383"
     CDS             5987..7396
                     /gene="glnG"
                     /locus_tag="JN691_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAY7243805.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            7508..7618
                     /gene="yshB"
                     /locus_tag="JN691_002384"
     CDS             7508..7618
                     /gene="yshB"
                     /locus_tag="JN691_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAY7243806.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(7847..9220)
                     /gene="hemN"
                     /locus_tag="JN691_002385"
     CDS             complement(7847..9220)
                     /gene="hemN"
                     /locus_tag="JN691_002385"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAY7243807.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(9409..9918)
                     /gene="yihI"
                     /locus_tag="JN691_002386"
     CDS             complement(9409..9918)
                     /gene="yihI"
                     /locus_tag="JN691_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAY7243808.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            10500..11132
                     /locus_tag="JN691_002387"
     CDS             10500..11132
                     /locus_tag="JN691_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAY7243809.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(11514..14300)
                     /gene="polA"
                     /locus_tag="JN691_002388"
     CDS             complement(11514..14300)
                     /gene="polA"
                     /locus_tag="JN691_002388"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAY7243810.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            14302..14544
                     /locus_tag="JN691_002389"
     CDS             14302..14544
                     /locus_tag="JN691_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243811.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSAGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFVFHAVKNKSIPNN"
     gene            14664..15596
                     /locus_tag="JN691_002390"
     CDS             14664..15596
                     /locus_tag="JN691_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7243812.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(15637..17067)
                     /locus_tag="JN691_002391"
     CDS             complement(15637..17067)
                     /locus_tag="JN691_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY7243813.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKNRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(17222..17848)
                     /gene="dsbA"
                     /locus_tag="JN691_002392"
     CDS             complement(17222..17848)
                     /gene="dsbA"
                     /locus_tag="JN691_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAY7243814.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(17865..18851)
                     /gene="srkA"
                     /locus_tag="JN691_002393"
     CDS             complement(17865..18851)
                     /gene="srkA"
                     /locus_tag="JN691_002393"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAY7243815.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHTGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(18928..19197)
                     /locus_tag="JN691_002394"
     CDS             complement(18928..19197)
                     /locus_tag="JN691_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAY7243816.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            19267..19851
                     /gene="mobA"
                     /locus_tag="JN691_002395"
     CDS             19267..19851
                     /gene="mobA"
                     /locus_tag="JN691_002395"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAY7243817.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            19833..20360
                     /gene="mobB"
                     /locus_tag="JN691_002396"
     CDS             19833..20360
                     /gene="mobB"
                     /locus_tag="JN691_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAY7243818.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         4969 a         5165 c         4951 g         5356 t
ORIGIN      
        1 gttctttatc gtccatatta ccaagagaaa aacgctcgcc atccagaaca tggattttga
       61 cgccacgtag attattttca cttgcgatat caagtaaatc agcaggcaac actctttcca
      121 gccgcatatt taaatgaaag gcataagcat gtaaataaag aggaagagtg tcgacacgtt
      181 gtagaatctt tcttgcgtta tttattgtca ccataaaaca cctacagatt aatgatgagg
      241 tctacggcct caaatcgcca atattcaata tctatctgga caatattgcc ttgctggtcg
      301 gcgcgatgct tttcaattaa gatcgctggc atacccgcag aaccgccaag atacttgctg
      361 atatcgcccg gcattctgac gggtttgaaa atcaattttt ttactaccgt ctctttttgg
      421 tagcactttt cccagactgc agaaaatgag tggttttcaa gttgttcaat aaaacccgga
      481 gcaacttcag gattaatata tgtttcgtga tagaaaactt tatgtccttc cagcgccccc
      541 cagccggtga tgcgatagag ttcagttgat ggcgtcactg caatcagtgg cgcgagcgtc
      601 tcgggaatat cgctggtacg gtttttctcg gtaaaccccc aagaaggctc tcgcccttgc
      661 tcaattgcgg cccgctgaaa gctcgccgac agctccggac tgtaattaaa tcgcggctgg
      721 gtcacgaacc accccttacg atccttacgg aagattttgg attctgcctc gaggttcaac
      781 aacgcctggc gcagcgtcat acgtttaatg cccagcagtt ctcccagctc tctttctgaa
      841 gggagttttc cacccggcgt ggtgatgcca tctttcaacc accgatccag tttctctttt
      901 gcattttcta cggtggattg attctccgcc attgtttaat ccttttgatt taaaccagat
      961 gaagcatctt acgcttacgc caaaaaagaa agcaattaac ggtgtatgac tttgcagata
     1021 attgtgacaa agctctctat tgacgtaatc acagacttat aagggagata ctcgcattgg
     1081 tgtgcgctca cgacaggtaa aaaaaacctg ccatcgctgg caggtttttt atgactaaaa
     1141 aacgaaatta atcgtctttc ggtgcgcggt tggcgcggcg acgatcgttt tccgtcaggt
     1201 gacgtttacg aatacggata gaggtcggag tcacttctac cagttcgtca tcatcgatga
     1261 actccagagc ttgttccaga gtcatgcgga taggcggaac cagaacaaca gcttcgtcag
     1321 taccggaagc acgcatgttg gtcagtttct taccggtcag gcagtttaca gtcaggtcgt
     1381 tagagcggct atgaataccg ataatctgac cttcgtaaac ttctgcaccg tgaccgagga
     1441 acagcttacc gcgatcctgc agaccgaaca gcgcgaacgc gacagcttta ccctgaccgt
     1501 tagagatcag tacaccgttc tgacgctgac ccacttcgcc cggacgtacg tcgtcgtagt
     1561 ggctgaaggt ggagtacagc agaccagtac cggaagtcat ggtcatgaac tcagaacgga
     1621 agccaatcag accacggctt gggatcacgt agtcgagacg tacgcggcct ttaccgtctg
     1681 gattcatgtt tttcaggtcg cctttacgtt cgcccagcgc ctgcatcaca gaaccctgat
     1741 gctgttcttc aacgtccagc gtcacgtttt catacggctc ttgtttacga ccgtcgattt
     1801 cacggaagat tactttcgga cgggataccg ccagttcgaa accttcacga cgcatgtttt
     1861 cgataagaac agagaggtgc agttcaccac gaccagaaac gcggaacgca tcggcgtctt
     1921 cggtttcttc tacgcgcagc gcaacgttgt gtaccagttc tttgttcaga cgatcgagga
     1981 tctgacgaga cgttacgaac ttaccttctt taccgcagaa cggcgaggtg ttaacgcaga
     2041 agaacataga aacggtcggc tcatcaacgg agagtgccgg cagcgcttca acgttttgcg
     2101 tgtcgcaaac ggtgtcagaa atgttcagtt cgcccaggcc cgtgatcgcc acgatatcgc
     2161 cagcttccgc cagatcggtt tcgatacgtt ccagaccgag gtggcccagc actttaccga
     2221 ctttcgcgtt acgggttttg ccttcgctat cgatgatagt aacctgctgg ttcggcttca
     2281 ctttaccacg cttgatgcgg ccaatgccga taacgccaac atagctgttg taatcgagct
     2341 gagaaatctg catctggaac ggaccgtcaa ggtcaacgtc cggcgcagga acgtggtcaa
     2401 caatcgcctg atacagcggg gtcatgtctt ccgccatatc ttcgtggtcc agacccgcga
     2461 taccgttcag cgcagaagcg taaacgatcg ggaagtccag ctgctcgtcg gtcgcgtcga
     2521 ggttaacgaa cagatcgaat acctgatcca caacccaatc aggacgcgcg ccagggcggt
     2581 caactttgtt gataacaaca atcggcttca ggccgtaagc aaacgctttt ttggttacga
     2641 agcgcgtttg cggcatcggg ccgtcaaatg cgtcaaccac cagcagcact gagtctacca
     2701 tggacattac acgttcaact tcaccaccga agtcggcgtg ccccggggta tcaacgatgt
     2761 tgatacggta atcattccat ttgatagcgg tgtttttcgc gaggatggta atcccacgct
     2821 ctttctccaa atcgttggag tccatcacgc gctcttgggt ttcggcacga gagtcgaacg
     2881 taccggattg ttggagcagc ttgtctacca gggtggtttt accatggtct acgtgcgcga
     2941 tgatggcgat attacgcaat ttttcgatca caactttgcc tcaggcatta gaaatagcgc
     3001 gttattgtac acggattaat cgcactacaa aacaggatca caaacatcct ccgcaaacaa
     3061 gtattgcaga gtccctttgt gatcgctttc acggagcata aaaagggtta tccaaaggtc
     3121 attgcaccaa tatggtgctt aatgtttcca ttgaagcact atattggtgc aacattcaca
     3181 tcatggtgca gcccttttgc acgatgttaa gcatgataac gccttttagg ggcaatttaa
     3241 aagttggcac agatttcgct ttatcttttt tacggcgaca cggccaaaat aattgcagat
     3301 ttcgttacca cgacgacaat gaccaatcca ggagagttaa agtatgtccg ctgaacacgt
     3361 actgacgatg ctgaacgagc acgaagtgaa gtttgttgat ttgcgcttca ccgataccaa
     3421 aggtaaagaa caacacgtca ctatccctgc tcatcaggtg aatgctgaat tcttcgaaga
     3481 aggcaaaatg tttgacggct cctcgattgg cggctggaaa ggcattaacg aatccgacat
     3541 ggtgctgatg ccagacgcat ccaccgcagt gattgacccg ttcttcgccg actccaccct
     3601 gattatccgt tgcgacatcc ttgaacctgg caccctgcaa ggctatgacc gtgacccgcg
     3661 ctctattgcg aagcgcgccg aagattacct gcgttccact ggcattgccg acaccgtact
     3721 gttcgggcca gaacctgaat tcttcctgtt cgatgacatc cgtttcggat catctatctc
     3781 cggttcccac gttgctatcg acgatatcga agccgcatgg aactcctcca cccaatacga
     3841 aggtggtaac aaaggtcacc gtccggcagt gaaaggcggt tacttcccgg ttccgccggt
     3901 agactctgct caggatattc gttctgaaat gtgtctggtg atggaacaga tgggtctggt
     3961 ggttgaagcc catcaccacg aagtagcgac tgctggtcag aacgaagtgg ctacccgctt
     4021 caataccatg accaaaaaag ctgacgaaat tcagatctac aaatatgtcg tgcataacgt
     4081 agcgcaccgc ttcggtaaaa ccgcgacctt tatgccaaaa ccgatgttcg gtgataacgg
     4141 ctccggtatg cactgccaca tgtctctgtc taaaaacggc gttaacctgt tcgcaggcga
     4201 caaatacgca ggtctgtctg agcaggcgct gtactacatt ggcggcgtaa tcaaacacgc
     4261 taaagcgatt aacgccctgg caaacccgac caccaactct tataagcgtc tggtcccggg
     4321 ctacgaagca ccggtaatgc tggcttactc tgcgcgtaac cgttctgcgt ctatccgtat
     4381 tccggtggtt tcttctccga aagcacgtcg tatcgaagta cgtttcccgg atccggcagc
     4441 taacccgtac ctgtgctttg ctgccctgct gatggccggt cttgatggta tcaagaacaa
     4501 gatccatccg ggcgaagcca tggacaaaaa cctgtatgac ctgccgccag aagaagcgaa
     4561 agagatccca caggttgcag gctctctgga agaagcactg aacgaactgg atctggaccg
     4621 cgagttcctg aaagccggtg gcgtgttcac ggatgaagcg atcgatgcgt acatcgctct
     4681 gcgtcgcgaa gaagatgacc gcgtgcgtat gactccgcat ccggtagagt ttgagctgta
     4741 ctacagcgtc taatagttga agttgtacta cccggcgcac aacgccggga tttagttgcc
     4801 gtggaaactt tcagcccatc tctgcatggg cttttttctc cgtcaattct ctgatgcttc
     4861 gcgcttttta tccgtaaaaa gctataatgc actaaaatgg tgcaacctgt tcaggagact
     4921 gctttatggc aacaggcacg cagcccgatg ctgggcagat cctcaactcg ctgattaaca
     4981 gtattttgtt aattgatgac aatctggcga tccattacgc caaccccgcc gcgcaacaac
     5041 tgctcgccca aagctcccgc aaattgtttg gtacgccgtt accggaactg ttgagctact
     5101 tctcattaaa tatcgagctg atgcaagaaa gtctggaggc ggggcaaggc tttaccgata
     5161 acgaagtgac gctggtcatc gatggtcgct cgcatatcct ttctgtgacg gcccagcgta
     5221 tgccggacgg catgatcctg ctggagatgg caccgatgga taaccagcgc cgcttaagcc
     5281 aggaacaact acaacatgct caacaagtag ctgcccgtga tttagtgcgc ggcctggcac
     5341 atgagattaa aaatccgctt ggcggtttac gtggcgcggc gcagttactc agcaaagcgt
     5401 tacctgaccc atcactactc gaatatacca aagtgattat cgaacaggcg gaccggctgc
     5461 gaaatttggt cgaccgtctg ttggggccgc agctgcccgg tacgcgcgtt accgaaagta
     5521 ttcacaaagt ggctgaacgc gtggtaacgc tggtgtcgat ggaactgccg gacaacgtgc
     5581 ggttgattcg tgattacgat cccagcctgc cggaactggc gcacgacccg gatcaaattg
     5641 aacaggtctt gctgaatatt gtgcgcaatg cgctacaggc gctggggccg gaaggcggtg
     5701 aaatcattct gcgtacccgc accgcgtttc aactgacctt acacggcgag cgctaccggc
     5761 tggcggcgcg gattgatgtg gaagataacg ggccgggcat tccgcctcat ttgcaggata
     5821 cgctgtttta cccgatggtc agcggccgcg aaggtggcac cgggcttggc ttatccatcg
     5881 cccgtaattt gattgatcag cattcaggca aaattgaatt taccagttgg ccaggtcata
     5941 ccgagttctc ggtttacctg cctatcagga aataaaggtg acgtttatgc aacgagggat
     6001 agtctgggta gtcgatgacg atagttccat ccgttgggtg cttgaacgtg cgctcgctgg
     6061 agcgggttta acctgtacga catttgagaa cggcgcggaa gtactggagg cgctggcgag
     6121 caaaacgccg gatgtgctgc tttcagatat ccgtatgccg ggaatggacg ggctggcgct
     6181 gctcaagcag attaaacagc gccatccaat gcttccggtc atcattatga ccgcacattc
     6241 cgatctggat gctgccgtca gcgcctatca acaaggggcg tttgattatc tgcccaaacc
     6301 gtttgatatc gacgaagcag tggcgctggt tgagcgcgct atcagtcatt accaggaaca
     6361 gcagcagccg cgtaatattc agcttaacgg cccaacgacc gatatcatcg gcgaagcgca
     6421 agccatgcag gacgtgttcc gtattatcgg tcggctttcg cgttcttcta ttagcgtgct
     6481 gattaacggc gaatccggca ccggtaaaga actggtcgct catgccctgc atcgccacag
     6541 tccgcgcacc aaagcgccgt ttatcgcgct gaatatggca gctatcccaa aagatttgat
     6601 cgaatcagaa ctgtttggcc acgagaaagg cgcgtttact ggcgcgaata ccattcgtca
     6661 ggggcgtttt gaacaggccg atggcggcac gttatttctc gacgaaattg gtgatatgcc
     6721 gctggatgtg cagacgcgtt tgctgcgcgt gctggcagac ggtcagtttt atcgcgttgg
     6781 cggctatgcg ccggtgaaag tggatgtgcg gattatcgct gccactcacc agaatcttga
     6841 acagcgggtg caggaaggta agttccgtga ggatctgttc caccgcctga acgttatccg
     6901 cgttcatctg ccgccgctgc gcgaacgtcg ggaagatatt ccccgtctgg cgcgccattt
     6961 tttacaggtt gccgcgcgcg aactgggcgt agaagcgaag ttgctgcatc cggaaaccga
     7021 agctgctctg acgcgtctgg cgtggccagg caacgtgcgc cagctggaaa acacctgccg
     7081 ctggctaacg gtgatggccg ccgggcagga agtgttgatt caggatttgc ccggcgaact
     7141 gtttgaatca acggttgcgg agagtacttc gcaaatgcaa ccggacagtt gggcgacact
     7201 tttagcgcag tgggcagaca gagcgctgcg ttccggtcat caaaatctgc tttccgaagc
     7261 gcagccagag ctggagcgga cgttactgac gaccgcgttg cgacatacgc aggggcataa
     7321 acaggaagcg gcgcggctac tcggctgggg ccgcaacact ctgacgcgta agttaaaaga
     7381 gctggggatg gagtgattca cggcttgtgt gtaaagattg attattgagc gcaaattgct
     7441 ggtattttgc gctttactgt tccgataagt tcagtatgat cttgcccgga aaacggggag
     7501 agtcattatg ctggaatcaa taattaatct ggtatcgagt ggcgcagttg acagccacac
     7561 accgcaaact gccgttgctg ccgtgctgtg tgccgcgatg attgggctgt ttagctgaag
     7621 ctcgttgttt atgccggatg cggagaaagt cgtaggcctg ataagacgcg accagcgtcg
     7681 catcaggcat catgctcaga tgcctgatgc ggcgtaaacg ccttatccgg cctacaaaag
     7741 caataatttc aacaggtttt gttgcccagc gcatcaggca atcttgttct cagcacattg
     7801 atggcgcttt cgttttctac tttgtaaacg aagcgccatt cactctttaa atcacccgtg
     7861 agaactgctg catccgcgct ttctggcgca gataggtatc aaagcacatg caaatgttgc
     7921 ggatcagcaa gcgacctttc gccgtcacct gtattccctt ctcatccaca tccaccagcc
     7981 catcttttgc taacggggcg agcagcttga gatcttccgc aaagtaatca gcgaagtgca
     8041 aatcccactg tttctcaaga ggggcgtaat ccagacggaa gttgcagatg agcgacttaa
     8101 tcacatcgcg gcgaatacag tcatcacgcg ttagcgcaat accacgccac agcgcattgc
     8161 cttgttcatc cacttgctga tagtactgct tcaactcttt ctggttctgc gcgtagcagt
     8221 cgccaatcat gctgatggcg gaaacgccca tccccagcag atcggtatcg ccctgagtgg
     8281 tgtagccctg gaagttacga tgcagcacgc cttcacgctg ggccaccgcc agctcgtcat
     8341 caggacgggc aaagtgatcc ataccgataa actgatagcc cgattgcgtc aggaaggcga
     8401 tggtttcctg caggatatcg agtttttgct gcggactcgg caggtcagca tctttgattt
     8461 tgcgctgagc agcaaaaatg gtcggcagat gcgcgtagtt aaaaacgctc agacgatcgg
     8521 ggttcagctc cgccacacgt ttcagggtaa aggcgaaact ctccggcgtc tgtttcggca
     8581 ggccgtaaat caggtcgatg ttggtggagg taaaaccaat ctcacgcgca tggttaagca
     8641 gtgcaaagat gaactcttca tcctgctcgc ggttaaccag acgttgcact tctttgttga
     8701 agtcctgcac gcccatgctc aggcgattaa agccttcggc gcgtaaatga tcgagtacat
     8761 ccagttcgat ttcccgcgga tcgacttcga tcgaaatctc cgcatcggta ttgaaatgga
     8821 agttttcgcg cagcagcttc atcagacggc tgatttgcgc tttattcaga tacgtcggcg
     8881 ttccgccgcc ccagtgcaat tggctgacgt gacgcccggc gaacagcggt gcacgatgga
     8941 cgatttcttg ctccagcgcg tccagatact gatcggcctt gtgctgctgg cgagtaacaa
     9001 tcttattgca accgcagaag taacaaagct tatggcagaa cgggatatgt acgtagagag
     9061 ataatggacg ctcaggatag cgcgccacgg cttgtaaaaa cgcctgttcg ccgaagtctt
     9121 ctgaaaactc cagcgcggtc gggtacgagg tgtatcgtgg cccggaatag ttatatttct
     9181 ggatcagggc cagatcccag tcgatttgct gtacagacat gctcactcct tccgatggcg
     9241 tctctggcgg ctacggcgta ccggcggatg cccgtttcgg gtcatcagcc ggttgcgcag
     9301 ccacttttgt cgccgcgaca accgctgtag tttaacgaat aaccacacca gataacatat
     9361 aaccggaagg gttatcagca gaacgggaag gcccacggcg gcaatccgtt agttgccccg
     9421 cagcagacgc atcatgtctt cttgcttctc gtcttcttcc tcttcttcgt catcgtcata
     9481 agagaggccg agtttctgca tcaactcatc aatacgatcc agtttagcat ccacccagga
     9541 ttgctcttcg gcgctcaggg tttcgcctgc ttccagacgt tccagcagcg catccagacg
     9601 ctcatccgtt tccagtaatt ccaactccgc ctgcggtgaa agcataggtt tctcactctt
     9661 cggtttgtgc tgtttggtga ctttttcagt cactcccaat ggaatagggg ttttactgcc
     9721 aatacgtgga tcttttggtg cgttctggcc tttgctgcct gacgtggtgt ttccgcccgc
     9781 tgcgcggctg cccggcgcat gaccacgacg ttttttctga cgcttacggt cacgagcttc
     9841 ctgatccagc tcctcgcgtg tttttcgacg cgcttttgca tgacctttgc tgcgtgagtt
     9901 tgaagatgat ggtttcataa tggttcgtct tttagccgtt ttattcagta tagatttgcg
     9961 gcggaatcta acagaaagca agcaaagaaa aaaggcgaca gattactctg tcgccttttt
    10021 tcctgactca taacccttaa cgggtcttac aatccttgca ggccgccaac acaccctgtt
    10081 tttccattag ccggaacaat ccgtgttgat tccatttccg tttaattacg tctccggacg
    10141 tttgtcttcc tgacaatcct ctgtcttcgc ctcctggcgc tcctgaccct catcctgagt
    10201 cattgttcct gttagcgtcc tcgctctatg ggtgctactt tacgcctttg ctttaaacaa
    10261 acaatcaacc gccatcagta tttgcacatt ttcagagttt tatataaaag caattcactg
    10321 atttatatag cttatttgac atgcatcgca gaattttctc tgctatttcg cataaagact
    10381 atcggcaatg tctcacaaag cgtatggctt ttacttacaa cgctcacgcc gaaaaatccg
    10441 ccttttatta tggttcaggt ataatcgcca caaagctttg actgacggag acgaccgctt
    10501 tgactaattt gaattatcaa cagacgcatt ttgtgatgag tgcgcctgat attcgccacc
    10561 taccttccga taccggaatt gaagtggctt ttgcaggccg ttccaacgca ggtaaatcca
    10621 gcgcgctgaa cacgctgact aaccagaaaa gcctggctcg tacctcaaaa accccagggc
    10681 gcacccagct tatcaacctg tttgaagtgg ctgacggcaa gcgtctggtt gacttgcctg
    10741 ggtacggtta tgcggaagtc ccggaagaga tgaagcgcaa atggcagcgt gcgctcggcg
    10801 aatacctcga aaaacgtcag agcctgcaag gtctggtggt gctaatggat attcgccatc
    10861 cgctgaaaga tttggatcag cagatgattg agtgggcggt agacagcaat atcgccgttc
    10921 tggtgctgct gaccaaagcg gacaaactgg caagcggcgc acgtaaagcg caattgaata
    10981 tggtgcgtga agctgtactg gcgtttaacg gtgatgtgca ggttgaaacg ttttcttcgt
    11041 tgaagaaaca aggcgtagac aagctgcggc agaaactgga tacctggttt agcgagatgc
    11101 agcctgtaga agaaacgcag gacggcgaat aattttcttg ccttaatgct tgtgccggat
    11161 gtggcgtatc cggcccgtaa attcaataga ttacaattct accgttggcc tgataaacat
    11221 agcgcatcag gcattacgga tcttttcttt cgcccaataa aaaacgcccc agtcattact
    11281 gactggggcg gctaaaatat tcagccaaat ccgattacgt gaagtaaaag gtctgaaaga
    11341 tagaacatct tacctctgta ccctacgcga ctaactttac tcttttttgt tcagttcaga
    11401 aagcactttt tgtaattttt tttcagttgt tgcataggaa tttctaatag ccatcacaaa
    11461 acgtgacagc ttatgttgct tacttacgaa aaaagggcat gttcaggcga atcttagtgc
    11521 gcctgatccc agttttcgcc actccccact tccaccagca acggcacatc cagacgggta
    11581 cagttttcca tcagttgatg aatctgcttc gcgacggcat caacatcatc tttatgaact
    11641 tcaaatacca gttcatcgtg tacctgcatg atcatacgta cacgcggttg ctcagcctgt
    11701 aaccacgcat caacggcaat catcgcccgt ttgataatgt cggcggcggt tccctgcatt
    11761 ggcgcgttaa tggctgcacg ttcagccgct gcacgacgag caccattgct ggatttgata
    11821 tccggcagat acagacggcg tccgtccagc gtttcaacgt agccctgctc tttcgcctga
    11881 gcacgggtgc gttccatata ctccagcacg ccagggtagc gttcgaagta aaggtccatg
    11941 tacttctgcg cttctttacg tggaatgttc aattgccgcg ccagaccgaa agcactcatg
    12001 ccataaatca gaccaaagtt gatcgctttc gcgctacggc gttgctcgct ggtgacggtt
    12061 tccagtggca aaccaaacac ttctgccgcc gttgcccggt ggatatcttt tccttccgcg
    12121 aatgcggtca gcaagccttt atcacgcgag agatgcgcca taatgcgcag ttcaatctgc
    12181 gagtagtccg ctgagacaat cacataatcc tctggcgcaa taaacgcctg gcggatacga
    12241 cgaccttctt cgttacgcac cggaatgttt tgcaggttag gatcggttga cgataaacgt
    12301 cccgttgcag ttactgcctg gtgataagag gtatgcacac gcccggtttt cgggttgatc
    12361 atcagcggca gcttgtcggt gtaagtcgac ttcagcttcg ccagaccacg atactccaga
    12421 atcacttttg gcaacggata gtccagcgcc agttcttcca gtacctcttc cgacgttgac
    12481 ggcgcgccac ccggcgtttt cttcagcggt ttaataccct gtttttcaaa cagaatggtt
    12541 tgcaactgct tggtggaaga aaggttaaat tcttcacctg caatttcatg cgctttcttt
    12601 tccagctcag ccagacgcaa ggtgagctct tcagaatgat tgtgcagcac tttcggatcg
    12661 atcttcacac cgttacgttc aatgcgtgaa agcaccggca ccagcggcat ttcgatattc
    12721 tcgaagacgt tcaacggccc tttgtgtttt tgcagatccg gccacatttt cagatgcaac
    12781 tgcaaggtga catctgcatc ttcggcggcg taacgtccgg cctcttcgag ggcaatctgg
    12841 ttaaaggtca gttgattttt gcctttaccc gcaatctctt caaaagtgat ggttttgtgc
    12901 ttcaaccaac gttccgcgag gctgtccata tcgtgacgcc cggcaacgct attgagaatg
    12961 taggactcca gcatggtatc aaacgcaatc ccacgcagtt caatgccgta attcgccaga
    13021 ataccgcgat cgtatttcag gttttgcccg accttcagcg ccttttcatc ttccagcagc
    13081 ggttttagca actcgagtgc acgctcgtga gagatttgat cgggcgcatc aagataatca
    13141 tgagcaaccg gaatatatgc cgctacgcct ggctcgatag caaaagaaag cccgaccagg
    13201 ttagcagaga tgttatcaag gctgtcggtt tcggtatcaa atgcaaatac cggcgctttt
    13261 tccagcttcg caatccacgc tttcagtgtt tcttcatcaa ggatagtgac gtagttgtca
    13321 taagaaatca ccgttgccgt cacttctggc gcttcatctg caacactggt ttcctgcggc
    13381 ttcgcggctg gttttgcccc tttggcctgt aaccatttgc ccgcttcgac atcagcagtc
    13441 cagcgtttga actcatactt tttgaacagc cccaacaact cttccgctgc cggttgctgc
    13501 acttccagtt gttcacaggt cagctccagt tcaacatcgg ttttaatcgt cgccaactgg
    13561 tatgagagat aagcaacttc tttgttttgc tcgagcttcg ctgccattgt tttcgcgcca
    13621 cggaagctca acccagcaat tttttctggc tcggcataca gcgtatccag cccgccaaga
    13681 ccttgcagca atgcctgcgc ggttttttca ccgacgcccg gtacgccagg aatgttatca
    13741 gaggagtcac ccatcagcgc caggaagtcg atgatcaatt ctggcggcac gccgtactta
    13801 ttcaccacct cttccggtcc gaggatggta ttcgtcatgg tattgataag cgtaatattt
    13861 ggcgtcacca gctgcgccat atctttatcg ccagtgctaa tcagcaccgg acgcccggct
    13921 ttttcggctt cgcgcgccag agtaccgata acgtcgtccg cttctacgcc agaaaccgcc
    13981 agcagcggca gtcccatcgc tttaaccatc gcgtgcagag gttcgatttg tgctcgcaga
    14041 tcgtccggca ttggcgggcg atgtgatttg taatgttcaa acagttcatc acgaaaggtt
    14101 tttcccttgg cgtcaaagac caccgctgca tgcgtcggtt tatattgcat gatcagactg
    14161 cgcagcatgt tgaggacacc atacatcgca ccggtcggct cgcctgcgct gttagtcagc
    14221 gggggaaacg cgtgatatgc gcgataaaga taagatgaac catctacaag gataagtgga
    14281 ttttggggga tctgaaccat aatgtccgtg cctgtttatc agattatggg taaaggatgc
    14341 cacagacagg atgaaaacat gagtttttca ccgcttttcg tgaaaatctt ttgtagatct
    14401 gctggatcgc tcgcaaatcg tcatgtggat aactttgtgc ataaattatt tacatcgctt
    14461 ttaatttatt atcatgcttt ttacagcatg atgtttgtct ttgtttttca tgcagttaaa
    14521 aataaatcca tccctaataa ttgagataat gggatttttt ctaatgtgga tagatatgaa
    14581 ttatttttct ccttaaggat catccgttat ttgggtcatt ttctgctaaa atctgcgcct
    14641 tgcgcttctt tagtgcactg tctatggcta accttttgaa taaattcatt atgacgagaa
    14701 tactcgctgc gataaccctt ttgctgagta tcgtattgac cattttggtc actatttttt
    14761 gttccgtccc gatcatcatt gccgggattg taaaactatt gctacctgtg ccagtcattt
    14821 ggcgaaaggt ttcgcgtttt tgtgatttta tgatgtattg ctggtgtgaa ggtctggcgg
    14881 tattactgca tctgaaccca cacctgcaat gggaagttca cgggctggaa gggttaagta
    14941 agaagaactg gtatctgctt atttgtaacc accgtagttg ggcagatatt gtcgtactgt
    15001 gcgtgctgtt tcgtaagcac attccgatga ataaatattt cctcaagcaa cagctggcct
    15061 gggtgccatt tcttggcctg gcgtgctggg cgctggatat gccgtttatg aagcgttatt
    15121 cccgcgctta tttattacgt catccggagc gacgtggtaa agatgttgaa accactcggc
    15181 gttcttgtga aaagtttcgc ctgcatccca ccaccattgt caatttcgtt gaaggctccc
    15241 gtttcacgca agaaaaacat cagcaaaccc actccacttt tcaaaacttg ttgccaccaa
    15301 aggcggcagg cattgcgatg gcgctaaatg tactgggtaa acaattcgat aaactgctta
    15361 atgttacgct gtgttatccg gacaataacc gtcagccgtt cttcgatatg ttaagcggta
    15421 aactgacgcg gattgtcgta catgtggatt tacagcctat tgccgatgag ttacatggcg
    15481 actacatcaa tgacaaaagc tttaagcgcc attttcagca atggctgaac tcattgtggc
    15541 aggaaaaaga ccggcttctg acgtctctca tgtcatcaca gcgtcagaat aagtaagttt
    15601 tactcgcctc tgaatgagca gaggcgagtg agtattttaa tgaacaaaac gtccggcacg
    15661 agacataaat tcttcttcgc tcatctcctg tccgttaaaa acaactttac ccggtgtata
    15721 acgcagctgc agcgaggcgg tattgttgtc aatcgtgatt aactgaaaca tctgacctaa
    15781 tgtcatcatc ccgctgattt gtttatcagc ctgcttttgc gctttttccg catccattcc
    15841 ttcagataaa ttaagctgtt ttgcggtttc tgtgacgaca ttaagcggta acttcacatc
    15901 aaaattgagc gatttgatat ctttgtttgt ggatgatgaa gatttggctg ggtcggcaat
    15961 actgatatcc agattggcat ttagttcgcc gagtgcgttc ttccatctta ccggttgttt
    16021 aatggtcggc tcactttttt gtaataacgg taaatactct ttgaacaatg cagcattcac
    16081 ttcttgcaga gcaacttcat cgtttgctaa ctcagggtgt gcagcaagct gcttctgcat
    16141 cgcaatgtta tactggatga taaattggcg cactgctgag ggatcaatag attcagcaat
    16201 caatgaccac tcaccactac cgagttgctg attccctttt gttaacttat caagttcgta
    16261 ggtcagattg acattgatat aatctttgtc tttaacgcga tccagcctgt tttttgcaac
    16321 gaaagcatcg atttgggcaa cgtcttcccc gtgattgata atgttaatct gattaaattt
    16381 cgactcgctt tcaccaaccg gaaatttctt ttcttctaat cgcgccagag aatcaatggt
    16441 aaaacttttt gccgttactt tgttcattgg tgataattgc agaacaatat cttcgacttt
    16501 gccttcaata tccaggttag acaatgaact accatcacca ttaaaagtaa actggccctc
    16561 accaaaagaa aactttccat actctgcagg ggcaacatct aatgtcgtag agaatgagcc
    16621 accaaacgcg aatcgcaatg tcgcttccac gggcgattta tttttcgcgg cgataaatag
    16681 cggttgtgtc agttcattat tcactaagtt cacttttgcc gctgccagtg ctgggatgac
    16741 attaccatgc attaacattg tgatgggcaa cggaccgtgc tcaacatcca catcaaatac
    16801 aactttctgg cctggcttga tattgagatc gggtgcaccg ttatcgaatg tcatttgcat
    16861 ttgaaaacgg gaattaaaaa agcctttgtc aaagttttta taacttaacg tcatatcgta
    16921 ggcatgttca cctttctttt tcgcatcgtt aaaatcttta ataaactttt cgataccagg
    16981 ctgaatttgc gtacctgtgt accatgtgcc accaccccag attacggcta acgcaacaat
    17041 aacacctgta gctgacttac gtatcatata aatattccct gataaaacag caaaactttg
    17101 aatatccact tatgctgaaa taatgtccag cggcaggatg cattatcaga atgtatttat
    17161 atttattaaa taataaaaaa agcccgtgaa tattcacggg ctttatgtaa tttacattga
    17221 attatttttt ctcggacaga tatttcaccg tatcagcata ctgctgaaca aaaacatcca
    17281 tattgctggt atccataccc tgcggattca gctgatattt accgttaaca aacatcgccg
    17341 gaacaccacg caattgcaca tcagctgcgg ctttttcctg ctgagcgacc agagatttca
    17401 ccacgaagct gttccacgcc gcgtcgtact cttcaccttt aatacctgca ttgataaata
    17461 catcgcggat atcagatgct gaacgaatgg tctgggtttt ctgtacgcct tcaaacagcg
    17521 gaacagtcac tttgtcttcc acgcccagcg ccatcgccac agcccatgcc tgagtcagat
    17581 ctttgcccag gtcaccaccc atgaagttga cgtggtattt agtcatcttc acgccttccg
    17641 gcagtttttt cttcacatta tcagaaatat gcagaacttc ttcaaactga tagcagtgcg
    17701 ggcagaagaa agagaaaaac tccagcactt gcggcgcgcc agctaccggt ttttccaggg
    17761 tagtgtactg tttaccatct tcatactgcg ccgccgatgc gctaaacgct aaaactaaac
    17821 cagccagcgc cagccaaatc tttttcatga tctactctct ccgattaata cataggtgtt
    17881 aattgcaaag ggggttcttg tagaactttt gcctgttcta taaaagtcgc cgtctgtcgt
    17941 agccagtaat cttccccggt taaccacggg aaatttttcg ggaacgcggg atcagcccaa
    18001 tgccgcatta gccaggcaag ataataaacc aaacgcatgg cgcgtaaagg ttcaatcagt
    18061 ccgatttcag cggtgtcgaa ctcgctaaat tcttcataag cttcaataat agtttccagt
    18121 tgcatccgct gctcggcttt atcgccattg agcaacatcc acaaatcctg aatagctgga
    18181 ccattacgtg catcatccag atcaacaaac attggaccat cgcgccagag aatattcccg
    18241 gtgtggcagt ctccatgtag ccgcagaacg gtgaaatctt cccgccagtg tgctgtaacg
    18301 gcggcgatca gctcatctgt cgctttcaga aatgccgctt tcaacccgga aggtatcagt
    18361 gtagcgtcct caaacagctt gcgaggctca atgagatatt cattcagacc gatggtcggg
    18421 cgatgaataa aaagctgttt gcgccccgtc tggtgcatac gccctaaata acgcccaacc
    18481 gcttccatct gatcgatatt atcagcttcg aactggcgac caccgacgct tggaaaaaca
    18541 gcgaaataaa atccctgatg attcaataaa gtctgaccgt taaaggccac aggtgctgcg
    18601 accggaactt catcatttac cagctgcaac gcaaattgat gttcttcgag gatttgatcg
    18661 gctgtccaac gttcagggcg ataaaatttg acgacaaaac gtcgacgctc ttcgtcctga
    18721 aattgataga cacggttttc atagctgtta agcggggtaa gaccggaatc cacccggatc
    18781 ccttgctcaa acagagcgtc catgatggta tccgggtgta gtgtctggaa agtaaaagcg
    18841 ctgttgttca tcccatcatc cggaaaacat tacgaatgat gaaggatatc atttcacgat
    18901 gattttggtg gcgccgctta caagctttta ctctttaatt acgccgcgag cgcgtaacag
    18961 cgccgttttg aagtcttctt cataatcttt ctgcaaaccc gggatcaccg catctttcgc
    19021 ggaatcacgc attttcaggt gatagatcag aatgtcatcc gtcaaatccg ccagttcgcc
    19081 gtcaaaacct gactctttcg ccagtttctg caaaaattgc aacaagttaa agtctggctc
    19141 tttttgccag gctggctgga ggagttcaat aacttcattc agacgtttac atttcatagt
    19201 aatgctcctt actcttgaga cagacacgtt agcagggtca atcccacaat aaaagaggcg
    19261 atatcggtga atctgatgac gacgataaca ggcgttgtgc tggcaggcgg taaagccaga
    19321 cgaatgggcg gcgtagataa aggattgctg gacttaaatg gcaaaccgct gtggcaacat
    19381 gtcgctgacg cgcttaagac gcagctctct cacgtcgtgg ttaatgctaa tcgtcatcag
    19441 gaaatctatc aggcaggcgg tctgaaagtg attgaagatt cactggcgga ttacccaggc
    19501 cctctggcag gaatgctttc agtaatgcag caggaagctg gtgagtggtt tttgttttgt
    19561 ccgtgcgata cgccttacat tccccatgat ttagccgccc ggcttaatca tcagcgcaaa
    19621 gatgcgcctg tcgtgtgggt ccatgacggt gaacgcgatc acccgactat tgctctggta
    19681 aaccgcgcta ttgagccttt attactggaa tatctgcaag caggagaacg ccgggtaatg
    19741 gcatttatgc gtctggctgg cggtcatgcg gttgatttca gcgatcataa agatgcattt
    19801 gttaacgtga atacgccaga ggagcttgcc cgatggcagg aaaaacgatg ataccgttac
    19861 tcgcctttgc cgcgtggagt ggtaccggaa aaactacgct tttgaaaaaa ctgatcccgg
    19921 cattatgcgc cagagggatc cgtccggggc tgattaagca tacgcaccat gatatggatg
    19981 ttgataagcc aggcaaagat agctatgagc tgcgcaaggc tggcgcggca caaaccatcg
    20041 ttgccagcca gcaacgatgg gccttgatga cggaaacacc agacgaagaa gagctggatc
    20101 tacattttct cgcaagtcga atggatacct caaagctgga tttgattctg gtcgaagggt
    20161 ttaagcatga agagatcgca aagattgtgc tgtttcgcga tggagccgga catcgacctg
    20221 aagaattagt gatagacagg catgttattg ctgtagccag tgatgtgccg cttaatcttg
    20281 atgtcgcatt actggatatt aatgatgttg aggggctggc tgattttgtg gtggagtgga
    20341 tgcaaaagca gaacggataa gcaagagtag gctggatcag acgcagccag tgtcgcatcc
    20401 gacctttttc tgcaccagcg caaaaaggcc cgactgggcc t
//
LOCUS       DADLSA010000068        20374 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.068, whole genome shotgun sequence.
ACCESSION   DADLSA010000068 DADLSA010000000
VERSION     DADLSA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.068"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(178..2499)
                     /locus_tag="JN691_002397"
     CDS             complement(178..2499)
                     /locus_tag="JN691_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAY7243819.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     GTERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLVPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(2596..3108)
                     /gene="greB"
                     /locus_tag="JN691_002398"
     CDS             complement(2596..3108)
                     /gene="greB"
                     /locus_tag="JN691_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAY7243820.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANW
                     YVNAIEYVKP"
     gene            3300..4019
                     /gene="ompR"
                     /locus_tag="JN691_002399"
     CDS             3300..4019
                     /gene="ompR"
                     /locus_tag="JN691_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAY7243821.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            4016..5368
                     /gene="envZ"
                     /locus_tag="JN691_002400"
     CDS             4016..5368
                     /gene="envZ"
                     /locus_tag="JN691_002400"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAY7243822.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5449..7071)
                     /gene="pckA"
                     /locus_tag="JN691_002401"
     CDS             complement(5449..7071)
                     /gene="pckA"
                     /locus_tag="JN691_002401"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAY7243823.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7450..9174
                     /locus_tag="JN691_002402"
     CDS             7450..9174
                     /locus_tag="JN691_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAY7243824.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWALWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(9309..10187)
                     /gene="hslO"
                     /locus_tag="JN691_002403"
     CDS             complement(9309..10187)
                     /gene="hslO"
                     /locus_tag="JN691_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAY7243825.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDSKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(10212..10613)
                     /gene="hslR"
                     /locus_tag="JN691_002404"
     CDS             complement(10212..10613)
                     /gene="hslR"
                     /locus_tag="JN691_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAY7243826.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(10624..11292)
                     /gene="yrfG"
                     /locus_tag="JN691_002405"
     CDS             complement(10624..11292)
                     /gene="yrfG"
                     /locus_tag="JN691_002405"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAY7243827.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(11357..13492)
                     /gene="igaA"
                     /locus_tag="JN691_002406"
     CDS             complement(11357..13492)
                     /gene="igaA"
                     /locus_tag="JN691_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAY7243828.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPSPTGASAAPLSLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATGVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            13812..14372
                     /gene="nudE"
                     /locus_tag="JN691_002407"
     CDS             13812..14372
                     /gene="nudE"
                     /locus_tag="JN691_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAY7243829.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(14537..17089)
                     /gene="mrcA"
                     /locus_tag="JN691_002408"
     CDS             complement(14537..17089)
                     /gene="mrcA"
                     /locus_tag="JN691_002408"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAY7243830.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITHKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLGQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            17209..17988
                     /gene="hofM"
                     /locus_tag="JN691_002409"
     CDS             17209..17988
                     /gene="hofM"
                     /locus_tag="JN691_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAY7243831.1"
                     /translation="MAFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            17988..18527
                     /locus_tag="JN691_002410"
     CDS             17988..18527
                     /locus_tag="JN691_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HAY7243832.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            18511..18951
                     /gene="hofO"
                     /locus_tag="JN691_002411"
     CDS             18511..18951
                     /gene="hofO"
                     /locus_tag="JN691_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAY7243833.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            18941..19345
                     /gene="hofP"
                     /locus_tag="JN691_002412"
     CDS             18941..19345
                     /gene="hofP"
                     /locus_tag="JN691_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HAY7243834.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNYVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            19257..>20374
                     /gene="hofQ"
                     /locus_tag="JN691_002413"
     CDS             19257..>20374
                     /gene="hofQ"
                     /locus_tag="JN691_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAY7243835.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRDLGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQ"
BASE COUNT         4614 a         5284 c         5538 g         4938 t
ORIGIN      
        1 ttaattaagg tttcagttgt taatgaagtg ataaatatta acggagtata ttgtgagttt
       61 ggctgtggaa taaaagtgcc ggagatattc tccggcactt ttatgtcagg caaagcgttc
      121 gttgccagat gcggcgtgaa cgccttatcg cgcaggttta ggggccagtc cgggcgttta
      181 acgtttttta cccatcgctg ccgccagcgc atccatcatc gcgctattac cggcaggctg
      241 cgcttcacga ccgcgtggtt tggctgccgg gcggttgttt tgcgggcgtt cattaccgcc
      301 gccgcgacga gagttggttt cgccaggctg ctcgtccaga cgcatagtca gggcgatacg
      361 tttacgctga agatccactt ccagcacttt caccttcaca atgtcgcccg ctttcaccac
      421 ggtatgcgga tcttccacaa acttgttcga caatgaagag atgtgaacca ggccgtcctg
      481 atgcacgcca atatcgacaa acgcgccaaa gttggtgacg ttggtcactg cgccttcgag
      541 gatcataccc ggttgcaggt cgttcattgt ctcgacgcca tcggcaaact gagcggtttt
      601 aaattccgga cgcggatcgc gacccggttt ttccagctct ttgatgatgt cagttactgt
      661 cggcacaccg aatttttcat cagtaaagtc agacgctttc aggttacgca gttcgctgct
      721 gttacccatc agatctttca gtgcctgctg tgttgctgcc agaatgcgtt ccaccaccgg
      781 ataggcttcc ggatgaacgg tagaggcgtc cagcgggtta tcaccgtggt taatgcgcag
      841 gaagcccgcg cactgctcga aggctttcgg caccagacgg ctgactttca gcagttgctg
      901 acggttctgg aactggccgt tctcatcgcg ccaggccacg atgttttgcg ccatcatgcg
      961 cgtcaggccc gccacgcggg ttaatagcgg aacagaagcg gtgttgagat cgacgccaac
     1021 ggcgtttacg cagtcttcta ctactgcatc cagtttgcgg gccagttgcg tctggctgac
     1081 gtcatgctga tactgaccta cgccgataga tttcggatcg attttcacca gctccgccag
     1141 cggatcctgc aaacggcggg cgatagatac cgcaccacgc agcgaaacgt cgagatccgg
     1201 gaactcctgc gctgccagct cggaagctga gtaaaccgac gcgccagctt cgctgacgat
     1261 cactttctgt gcggtcactt tcgggaactg cttctgcacg tcgagataga aacgttcagt
     1321 tccgcgggaa gctgtaccgt tgccgatcgc caccagttca acgttatgtt tttcacacaa
     1381 ggcagcaacg gtcatcgctg cttttgcggc ttgtccggtg tgcgggtaaa tggtatcggt
     1441 cgccaccagt ttgccagtgg catcgaccac cgccactttt accccggtac gcagacccgg
     1501 atcgaggccc atcgttgcac gcagtccggc aggggccgcc atcagcagat cgtgcaggtt
     1561 acgggcaaag acgttgattg cttcatcttc cgcgcgttcg cgcacggtac ccatcagttc
     1621 ggtttccaga tgcatcagca ccttgatgcg ccaggtccag ctcactacgc ctttgcgcca
     1681 gctatccgcc ggggcattgt tcaggcgcag gccaaggtga tccatgatga tttgctcgca
     1741 atagctctct ttgggcggct catcgaactg cggatcggca ttcagcgaaa gctggagtac
     1801 gccttcgtta cgcccacgga acatcgccag cgcgcggtga gaaggcaccg tggacaacgg
     1861 ttcgtgatga tcgaaatagt cgcggaattt cgccccttcc tcttctttac cgctcaccac
     1921 cgtagaaacc aaatgcgcgt tcttccacag ataatcacgc actttcgcca gcagcgcggc
     1981 atcttcggca aaccgttcca tcaggatata gcgcgcgcca tccagcgcgg ctttggtatc
     2041 tgccacgcct ttatcggcat caacatattg tgcagcggcg acttctggcg tgtgtgacgg
     2101 atcgctccac agcaggtcag ccaacggctc aagccctgct tcaatggcga tttgcccgcg
     2161 ggtgcggcgt ttaggtttgt agggcaggta gaggtcttcg agttcggttt tgcttagggt
     2221 ggcgttgatg gccttcgcca gatcatcggt gagtttgcct tgctcggaaa tggatttgag
     2281 gatcgcctga cgtctctctt ccagctcgcg cagatagctc agacgcgttt ccagattacg
     2341 cagctgcgtg tcatccagac cgccggtgat ttccttacga taacgtgcga taaacggcac
     2401 ggtattccct tcgtcaagca ggcgaacggc agcgtcaacc tgttccgggc gcgcctgaat
     2461 ttcacccgca ataatgcggc agaacgaatc attcatcatg gtttgagttc atcttttcgg
     2521 atcaaaaatc aggggatagt tatacggact ggctggcaaa aatgccagcc atcggcagga
     2581 ggttaagact cttccttacg gtttcacgta ctcgatagca ttaacatacc agttcgcttc
     2641 cccggcaggg gtattcacca ccgccagatc gccgacttct tttttcaaca atgcgcgggc
     2701 catcggggaa tcgatagaga tgtaatcttt acggccaaaa atttcatcgt agccgacaat
     2761 acggaaacgg tgagtcacgc catcgtcgtt ttcaatctcc acccacgcgc caaaaaagac
     2821 tttgccttcc tgctgagggg agtaatcgac gattttgaga ttttccaggc atttagtgag
     2881 atagcgcacg cgacggtcga tttcacgcag acgcttttta ttatactgat agtcagcatt
     2941 ttcgctgcgg tcgcccagac ttgcggccca ggtcaccttt tttgtgacct ccgggcgttc
     3001 ttcacgccag agataattaa gctcttgttt gagtttttca tacccttccc gggtaaccag
     3061 gggcgttttc atctcgttga ttccctttgt ctgtttgata atgcgcacat tgggtataac
     3121 gtgatcatat caacagaatc aataatgttt cgccgaataa attgtatact taagctgctg
     3181 tttaatatgc tttgtaacaa tttaggctga aattcatacc agatttagct ggtgacgaac
     3241 gtgagctttt ttaagaatac acgcttacaa attgttgcga acctttggga gtacaaacaa
     3301 tgcaagagaa ctacaagatt ctggtggtcg atgacgacat gcgcctgcgt gcgctgctgg
     3361 aacgttatct caccgaacaa ggcttccagg ttcgaagcgt cgctaatgca gaacagatgg
     3421 atcgcctgct gactcgtgaa tctttccatc ttatggtact ggatttaatg ttacctggtg
     3481 aagatggctt gtcgatttgc cgacgtcttc gtagtcagag caacccgatg ccgatcatta
     3541 tggtgacggc gaaaggggaa gaagtggacc gtatcgtagg cctggagatt ggcgctgacg
     3601 actacattcc aaaaccgttt aacccgcgtg aactgctggc ccgtattcgt gcggtgctgc
     3661 gtcgtcaggc gaacgaactg ccaggcgcac cgtcacagga agaggcggta attgctttcg
     3721 gtaagttcaa acttaacctc ggtacgcgcg aaatgttccg cgaagatgag ccgatgccgc
     3781 tcaccagcgg tgagtttgcg gtactgaagg cactggtcag ccatccgcgt gagccgctct
     3841 cccgcgataa gctgatgaac cttgcccgtg gtcgtgaata ttccgcaatg gaacgctcca
     3901 tcgacgtgca gatttcgcgt ctgcgccgca tggtagaaga agatccagcg catccgcgtt
     3961 acattcagac cgtttggggc ctgggctacg tctttgtacc ggacggctct aaagcatgag
     4021 gcgattgcgc ttctcgccac gaagttcatt tgcccgtacg ttattgctca tcgtcacctt
     4081 gctgttcgcc agcctggtga cgacttatct ggtggtgctg aacttcgcga ttttgccgag
     4141 cctccagcag tttaataaag tcctcgcgta cgaagtgcgt atgttgatga ccgacaaact
     4201 gcaactggag gacggcacgc agttggttgt gcctcccgct ttccgtcggg agatctaccg
     4261 tgagctgggg atctctctct actccaacga ggctgccgaa gaggcaggtc tgcgttgggc
     4321 gcaacactat gaattcttaa gccatcagat ggcacagcaa ctgggcggcc cgacggaagt
     4381 gcgcgttgag gtcaacaaaa gttcgcctgt cgtctggctg aaaacctggc tgtcgcccaa
     4441 tatctgggta cgcgtgccgc tgaccgaaat tcatcagggc gatttctctc cgctgttccg
     4501 ctatacgctg gcgattatgc tattggcgat aggcggggcg tggctgttta ttcgtatcca
     4561 gaaccgaccg ttggtcgatc tcgaacacgc agccttgcag gttggtaaag ggattattcc
     4621 gccgccgctg cgtgaatatg gcgcttcgga ggtgcgttcc gttacccgtg cctttaacca
     4681 tatggcggct ggtgttaagc aactggcgga tgaccgcacg ctgctgatgg cgggggtaag
     4741 tcacgacttg cgcacgccgc tgacgcgtat tcgcctggcg actgagatga tgagcgagca
     4801 ggatggctat ctggcagaat cgatcaataa agatatcgaa gagtgcaacg ccatcattga
     4861 gcagtttatc gactacctgc gcaccgggca ggagatgccg atggaaatgg cggatcttaa
     4921 tgcagtgctc ggtgaggtga ttgctgccga aagtggctat gagcgggaaa ttgaaaccgc
     4981 gctttacccc ggcagcattg aagtgaaaat gcacccgctg tcgatcaaac gcgcggtggc
     5041 gaatatggtg gtcaacgccg cccgttatgg caatggctgg atcaaagtca gcagcggaac
     5101 ggagccgaat cgcgcctggt tccaggtgga agatgacggt ccgggaattg cgccggaaca
     5161 acgtaagcac ctgttccagc cgtttgtccg cggcgacagt gcgcgcacca ttagcggcac
     5221 gggattaggg ctggcaattg tgcagcgtat cgtggataac cataacggga tgctggagct
     5281 tggcaccagc gagcggggcg ggctttccat tcgcgcctgg ctgccagtgc cggtaacgcg
     5341 ggcgcagggc acgacaaaag aagggtaaat aaacgggagg cgaaggtgcc tcccgtttcg
     5401 ttttgctttc tataagatac tggatagata ttctccagct tcaaatcatt acagtttcgg
     5461 accagccgct accagcgcgg cacccgcagg ggtgtcggtg tatttatcga agttgtcgat
     5521 aaacagtttc gccagggttt cggctttttc ctgccactgt tccggagaag cgtaggtgtt
     5581 acgcggatcg agaatcttcg tgtctacgcc cggcagttcg gttgggatcg ccaggttaaa
     5641 catcggcaga gtgaaggttt ctgcattatc cagcgaaccg ttgaggatgg cgtcgataat
     5701 ggcgcgggta tctttaatcg agatacgttt gccagtgccg ttccagccag tgttaaccag
     5761 ataagcctgc gcgcccgccg cctgcatacg tttcaccagc acttctgcgt actgagtcgg
     5821 gtgcagcgac aggaacgccg cgccgaagca agcggagaag gttggcgtcg gttcggtgat
     5881 gccacgctca gtaccggcca gtttggcggt gaagccagag aggaagtgat actgggtttg
     5941 atcggcagtc aggcgagaaa ccggcggcaa cacgccgaaa gcatcagcag tcaggaagat
     6001 aaccttagtc gcgtggcccg ctttggaaac cggcttaacg atgttatcga tgtgatagat
     6061 tggataagaa acgcgggtgt tctcggtttt tgaaccatca tcaaagtcga tagtgccatc
     6121 ttcacgcacg gtgacgtttt ccagcagtgc atcacgacgg atagcgttgt agatttcagg
     6181 ttccgcttct ttcgacagct tgatagtttt tgcgtagcag ccgccttcga aattaaacac
     6241 gccgtcatcg tcccagccgt gttcgtcatc gccaatcagg cggcgtttcg ggtcggtgga
     6301 aagggtggtt ttaccggtgc cggaaaggcc gaagaacacc gcaacatcgc ctttctcgcc
     6361 aacgttggcg gagcagtgca tagaagcgat acctttcagc ggcagcaggt agttcatcat
     6421 cgagaacatc cctttcttca tttcgccgcc gtaccaggtg ccgccaatca gctgcatgcg
     6481 ctcggtcagg ttaaacgcca cgaagttttc ggagttcaga ccctgttctt tccactgcgg
     6541 gttagtgcac ttcgcgccgt tcataacgat aaagtctggt ttgaaacctg ccagctcttc
     6601 atcgctcggg cgaataaaca tgtttttgac aaaatgcgcc tgccaggcca cttcggtgat
     6661 gaaacggacg gaaagacgag tatccgggtt cgcaccacag aaagcgtcga caacgaacag
     6721 acgtttgccg gaaagctgcc tggtcaccag gcctttcaga tgctgccagg tttccggaga
     6781 gagaggtttg ttgtcgttct tacctttgcc tttgtctgcc caccagaaag tatcgcgagt
     6841 ggtatcgtca cggacgatat acttatcttt tggtgaacga ccggtgaaga tcccggtatc
     6901 gacggcaacg gcacccagat tagttaacac cccgcgctca taacctgtca ggctcggatc
     6961 gagctcttcc tgatacagca ggtcgtagct tgggttgtaa acgatatcat gtacgtcact
     7021 gataccataa gcctcgagtt cttgcggggt caaaccattg ttaacgcgca tttcactgct
     7081 ccttagccaa tatgtattgc ctgaatagta aagtcttttt gggggtgtta accgcgacaa
     7141 ggctcataga tttacgtatc tggagaaatt catatttgac aaaaaactcc gtgattcctg
     7201 tcacgaaacg gttgctatta tcgcagaaat agcattttag gtgtggaaaa tgttctcaaa
     7261 aggttaaatc ttggtttata tggcgggaat attgtgagtg gaatcgcatt cctgaatgaa
     7321 attgatgcaa attcaaccgg taaagtatcg attcaccacg ctaacttttt ttgattattt
     7381 tctgaaaaaa gcaaaatact actgcccgga tgcgataatt aacccacctt ttgattctgg
     7441 ataaccaaca tggataacgt tgaactttca ccggcaacac gctgggggat gattgctacc
     7501 ggattacttc aggggctggt ttgctacctg cttatcgcct ggcttgccgg aaaaaatcac
     7561 agttggattg tttatggcgt gcctgcgacc gtcgcgtttt catccgtttt actcttctcc
     7621 gtgatctctt ttaaacagaa gcgcctttgg ggatggctgg cgctggtgtt tattgccacg
     7681 acagggatga gcggttggct gaagtggcaa actgatggca tgaacccctg gagagctgaa
     7741 aaggcgattt gggattttgg ctgctatctg ctgttgatgg caatgttgtt gctaccgtgg
     7801 atacaacaaa gccttcgtat ccgcaatggc agtagccgct atagctactt ttaccagtca
     7861 gtatggcata acgtacttat attactggtg atttttctct ccaatggcct gacgtggctg
     7921 gtgcttttac tgtggggtga gttgtttaaa cttgttggca tcacgttttt caatacgctc
     7981 ttttttgcaa ccgactggtt tatgtatctt acatttggtc tggttaccgc actggcggtg
     8041 atcctcgcgc gtacacagtc acgtttaatc gactctattc aaaagttgtt cacgctaatc
     8101 gccacggggt tgctgccgtt agtatcattg ctaaccctga tgtttatcat caccctgccg
     8161 tttacgggcc tgagcgcgat ttctcgccac atctccgccg ccgggttgct gttaacgctg
     8221 gcctttttgc aattgatctt aatggctatt gtccgcgatc cgcaaaaagc gtcacttccc
     8281 tggacggggc cgttgcgttg cctgattaaa accgctttgc tggttgctcc gctgtatgtg
     8341 ttcgtcgcca cctgggcgtt atggctgcgg gtcgctcagt acggctggac tgtcgaccgc
     8401 ttgcagggcg cgctggcgga gctggtgtta ctggtgtggt cgctggggta ttttgtcagt
     8461 atcgtctggc gtaatgggca aaatcccctt gttcttcagg gcaaagtgaa ccttgcggtt
     8521 tcgttattgg tgttggtgat actggtgctt cttaattcgc cggtgctgga cagtatgcgt
     8581 attagcgtga acagtcatat ggcgcgttat cagagcggca aaaacacgcc agaccaggta
     8641 actatctaca tgctcgaaca gagcggtcgc tatggacgtg cggcgcttga gtcgctgaaa
     8701 agcgatgccg ggtttatgaa agacccgaaa cgcgcgcggg atctgctgat ggcgttagat
     8761 ggagagcaac atcttcagga acaggtatcg gaaaaagtat tagccgaaaa tgtgttaatt
     8821 gcccctggtt ctgttaaacc ggatgcgaca ttctggtcgg ccttaatcca ggatcgctat
     8881 aacgtgatga cctgtattga aaaagacgcc tgcgtactgg tcgagcaaga tctgaatagt
     8941 gatggtcagg cggagcggat cctgtttgct tttaatgatg acagagtcat tgtctatggc
     9001 tttgactcag acagaaaaga atgggacgcg cttgatatga gtttacttcc gaacgaaata
     9061 acgaaagaaa aattactcac agctgcgaag gatggaaaac tggggacgag gccgaaagcg
     9121 tggcgcgatc tgacggtaga tggtgaaacg ctggaaataa acttaagtaa gtgacaacgc
     9181 ttttatcgga tacgacgctg tatctgacca aaataatttg cgatttattt cataggcgct
     9241 ggtaagcgca gcacatcagg ccattaagtc gtaaaatccg gcagagccct ctctgccgga
     9301 catactcatt aatgaacttg cggatctgcc ggagacgcgt tgttgcggat ttcagcaata
     9361 tccatcgcat tgaacagata gtggttaccg cagtaatcac aatgcatgtc aatttcgcca
     9421 tcttccgcca ggatgctatc aacttcttca tcaggcagcg ttttcagcgc atcggcgcaa
     9481 cgttcacgcg agcaggtgca tttgaactcc acatcctgcg gatcgtaaac cgtcacctct
     9541 tcttcgtgat acaaacgcca caacacttcg tttgccggta aggtcagcag ttcttcggtt
     9601 ttgatggttt cggttagcgt cgccaggtgg tcaaagtcgt cctgctgggt attttgcgca
     9661 ggcattacct gcaacaacat accgcctgca gccggtttgc tgtctacgtc gccggtgcga
     9721 ataaacaggc gcgtcggcag ctgttcagaa cgcataaagt aatcttccag gcaggccgcc
     9781 agggtatcac cttccagacc aactacgccc tgatagcgtt cgccttcgct cggggtaatg
     9841 gtgatcacca cgtaaccatt gccgaccagc gttttcaggt cggcattttc tggaatttcg
     9901 ccctgcacgc gcgccacacc gcgcatttgc tggttattgt taccgttaat aaccgccaga
     9961 ttcatcggac cgtcgccctg cagctgtacg gtgatatcac catcaaactt cagcgtagcg
    10021 gttaacaggc tggtcgcaac cagcagttct gccagcacgt ttttaacggg ctgcggataa
    10081 tcgtggttct caaggatctg ttgcagggtt tccgaaacgg ttaccagttc gccgcgaacg
    10141 gcaaaatttt caaacagata gcgatgtaat tggtcatgtt gcggcataat catctctctt
    10201 gcaggtgaca gttattcact gtcgccgtgt ttaaatcgta acaggtcgcg gcgctctttt
    10261 ttgtccgggc gtcggtccgg gtgcggcatg gttaaggcat taagtttacg tgccatcgcc
    10321 attttttcgc gtttctctac actttccgca gtctcttcat acagcaaggc tgcctcgctg
    10381 gcggggcgac gctgttcagt aatcgccttt acaatcaccg tgcgttcgtc atttccctgg
    10441 cgcagagtga gcgtggcatt cagctcgacg attttgctcg gcttgctgcg ctgcccgttg
    10501 taatgcacct taccgccttc aatcatttca cgggccagcg cgcgggtttt ataaaaacgg
    10561 gcagcccata gccatttatc cagtcgaacc tcaacagcag gtttctcttt catggcgtct
    10621 ccttcacatt agcgagggga tcaggcggcg gtagtcattc agtgacggat ggcgttgata
    10681 ctgtttctcg gcaatcccgg aatcaggatt agtcacgccg aggcagtaac gaataccaaa
    10741 ttgcgcggca gcatcgagaa tcgcttcgct gtcatcaata aacagcgttc tttcagcttt
    10801 cagacccgta gcttcggcca ccgcatgcca taaccgctga tcctctttcg gataaccaaa
    10861 tgtgtgggtg gaaagtaata aatcaaggtg tgcgtccaga cccgtatgct caagttttac
    10921 cgccaggttg tgcggatgag cattggtgag caaaattcgc tgcttaccgc tggctttcag
    10981 tgcctcaaga aacggaatgg tatcttcacg cagtacggcg cgcggtccca tctcggtggt
    11041 catcgcacag atatccagac ccagttgctc actccagtaa tcaagacagt accagtttag
    11101 cgtatgctgt acgtcgtgat attgctggcg catatattcc atcgcttcct gtggcgtaac
    11161 cccgtttttc gcgccccatg tttcaggcac cagcttttgc cagaaatagt tatcgaaggc
    11221 gaggtcgagc aacgtgccgt ccatatccag cagaacggta tctacgtcct gccaggcaat
    11281 gttgatatgc atgagggaaa tctccagagt gaagcaattt gcgcgacagg gtagcataac
    11341 ctgccgcgca aacgtgttat tcgataaggc tttctgaagg ggtgatcagt tgcgggttca
    11401 ggcagctttc ataataggcc tgaatctcca tcatgcgagt gcgatgacgc tggtaacgac
    11461 gccaggcctg tacgccattg taaatggcgc tacccagcat cgtcagtagc agcaatgtgg
    11521 tgctgagata gcgccacagg ccggaacgat ccgggatcgg atgaaggcca atatgctgcg
    11581 taccattggc gtcagtgaag atttttgtga cgataccttc ggcgttaaac ggcgtatgca
    11641 tcagcatttg tgccagtttc tggaaagcgt tccactgttc ttgcggcggg tagtcgtaaa
    11701 gtgatgccga aggccagggc tgatcaacaa aatcgctgcc ttcgtcgctg acaatcagga
    11761 atccgccggg ggccggactg tttagtgatt gtgccgctcg cgccgtttca tgcgtgataa
    11821 acggcgcggt ggaggttgcc accaggttat ccagcgattc cgcactcacc gggcgtaata
    11881 acacattcac gccatcgagc ttcccggcgt tggcgcgttt taccagcgcg tcccagtctt
    11941 tactgttgcc gagattgacc agcgcatttt tcagtcgcac acagtcatct ttggcagaac
    12001 ataaatccgc tgtcttcagt acaatgtcgc caaaatcatc aagcaatacc atgccggatt
    12061 tttgaatcgc tgaacgtaat gaggcactga cgcgagattc atcttccggt ttagggtgca
    12121 gctggcgatt aactgcttca gtcaatgccg tcgctttgtt gaccagttca gattctggta
    12181 atggtaatga gcgggcgtca ttccagatga tctgcgagca gtcaaacggt aaaaaaggtg
    12241 aattggtttt cgcgctccag gttccggaag tgcgaatatt acacattccc gtaccgctaa
    12301 tacgcaatgt atcgcctacc cgcacgccag cgtcagccag ttgttttacg ccggtggctt
    12361 caatggtctg cgcgcctttc atccatgaga gagtgaattt cagcggcata tccagcggga
    12421 tccagaataa cagcataaac agcaccagca gcgagcccgc agcgataatc gtactgcgca
    12481 gccagtgctg taacggaaag ttttttactt catcatgcag cgaaagatat cgcccctgac
    12541 gcactacgtg gcggtcgaga tagatatcaa tatcggtttg ttgaccgaga tcctgagcaa
    12601 tgtatggctg ccagtgtgcg ggatagacca ggtcgataat accgagtgaa atattgttga
    12661 tctgttcctg atcgttttcg ccaaacaatc cccaacgacg gggtgtaccg cgcagacaat
    12721 gaatttctcg cagggaggat tttgccgggg gcgcgaatag cccccacaga cctgcgccca
    12781 gcagcagtaa tgcgccgcct gccagccacg gaacaaatac atccggggta atcaggcaga
    12841 aaaagaacat caggaaggag gcgacgatca gcaacgcttc acgcagcccg cgcggacgac
    12901 tcagcgcata ttcttcatgc gtttctttgc gaatattgag tagctcgatt tgctcactct
    12961 cttccccgcg aatcgacgcc tgcgtggacg gaacaggttg caaggcatag ccgcgcgttt
    13021 cctgcatgta ctcctgcagc gtatgaccgt tgagtgaaat aaccaacggc agcgaatcgg
    13081 tatgaatcag ttcaacggta ttctcatcgt tgatgtactg ttcccagaag gggggcaggt
    13141 ggacttctac cgaatcgagg tagtaacgcc atttattcgg atcgtcggta gagatgccgt
    13201 aacgtgtgat agcgtgtgtc agcatcatga cgttgttgct ttcggcattc agcgccagag
    13261 agagcggtgc cgcgctagct cccgttgggc taggcacctg taatacctgc gtcaggctct
    13321 caagataatt ttcaacggcg ctacgttctt caggtgtgag tttacgcgtt tgcgcatccg
    13381 cgaaggcgtt ggtccagggc agctgacgcc gtctggatcg cacttttatc agccatcccg
    13441 caagtagtga gcaggccagc aaagcagcta aaaaaatcac aatggtgctc atgctttccc
    13501 catcttactt agtctgtcag gcgtggtcag tcgcaccgga cacaatacat gattctgcgg
    13561 ttggctatcg gcaagcgtgg agtttaactt gagcatcatt aagcacatca cagtaaccgc
    13621 tgatcatagc aaaccctctg aaggcggaat atcagcaaat tcctggagtt atttcaacct
    13681 attgactttt aatttgattt gaggacgatt atttattaag ttacataaat gtaaataaca
    13741 tgcaattcac attgccgaaa gcgtgtccga tatcgcacaa taaccgctga tttcacagca
    13801 tagatcccac gatgagcaaa tcattacaaa aacccaccat tctgaatgtt gaaactgtag
    13861 cccgttcccg actgtttacc gtcgagagcg tggatctgga gttcagcaat ggcgtgcggc
    13921 gtgtttatga acgaatgcgt ccaaccaacc gggaagcagt gatgattgtg ccgattgtgg
    13981 acgatcacct gatcctgatc cgcgaatacg cagtgggaac tgaatcctac gaattaggtt
    14041 tttcgaaagg attaattgat ccgggtgaaa gcgtctacga agccgctaac cgcgagctaa
    14101 aagaagaggt tggatttggt gcgaacgatc tgactttttt gaagaagctc agcatggcac
    14161 cgtcttactt ttccagcaaa atgaatatcg tggtcgcgca agatctctac ccggaatcgc
    14221 tggaaggcga tgagccagag ccgctaccac aggtgcgctg gccgctggcg catatgatgg
    14281 atttgctgga agaccctgac ttcaacgaag cgcgtaatgt cagcgcgctg ttcctcgtgc
    14341 gcgaatggtt gaaagggcag gggcgagtgt aaatgcgtga agtttgcctg atgcacaacg
    14401 cttatcaggc ttatggggtt cctgcaatat attgattttt cacgattttg ttagccggat
    14461 aacgcgttca cccgcatccg gcataaacaa atgcactttg tcagccaact gaaaaggcgc
    14521 cgaagcgcct ttttaatcag aacaattcct gtgcctcacc attatcgata atggtcgttc
    14581 ccacctcgtg caccgcctgt tgtgtcggct gcgtaccttc gatgaaatac tcttcgcggc
    14641 tgttgccacc attagctaac tgcccggtgc tgcgatcgat attcaccgtc acaatacccg
    14701 gaggcggcgt cagcggctgc tccggcacac cttcaagaac ggctttcata taagcgtccc
    14761 atgcaggctg ggcactcttg gcaccgcctt cgtaacctga gatctgatct ttaatcgctc
    14821 cggaagccgt tgtatgaccg agattacgac ggtgatcatc aaagccaatc cagaccgagg
    14881 tcacaacgcc cggaccgtaa cccgagaacc acgcatcttt cgaactgtta gtggtaccgg
    14941 ttttcccgcc gatatcgcga cgctgcaaat cacgacctgc acgccagccg gtaccctgcc
    15001 agcctggttc accaaagata ttggtgttca aagcactctt aatcaggaat gccagcggag
    15061 tgttgatgac gtgcggtgcg tactcctgcg cgccagtctt cgccactaac gcctgatttg
    15121 cctgccccag ctgcggcatt ggtacagaaa cattctgctg ctcgcgggag atagcgacat
    15181 cttcaacatc gttattttcc agcacgttcg atttctgcgt atcaccgtaa atcactggaa
    15241 tatcgcattc cgggcaggct actttcggtt tcgcttcgaa aatcacgccg ccctgatcgt
    15301 tttcaatttt gctgataaac cacgggtcca ccaggaagcc gccgttcgcc atgaccgcgt
    15361 agccgcgcgc cacctgcatt ggggtgaagg acgctgaacc cagcgccagc gattcggtgt
    15421 ggacaatgtt ttgcgccggg aagccgaagc gttgcagata ttctgcagcg tagtcgacgc
    15481 ccatcgcccg cattgcacgt accatcacca cgtttttcga ctgacccagc ccctgacgta
    15541 agcgaattgg accagcatac tgcggtggtg agttcttcgg ctgccagtca gaaccggcac
    15601 ctgcatccca gcgagaaatt ggcacatcgt tcaacatact tgccagcgtc agacctttat
    15661 ccatcgccgc ggtgtagagg aacggtttga tgttggaacc cacctgacgc agtgcctggg
    15721 tggcgcggtt aaacttgctc tgattgaaat caaagccacc gaccagcgcc ataacggcac
    15781 cattttgcgg attgatcgac accagcgccg agttcacttc cggcacttgt gccagccacc
    15841 atgcatcgcc aacctgacga acccagattt gctgacccgt ttgcagaaca tcggtcactt
    15901 tacgcggcgt cggtccctgc tgagtatccg aacggtaagg acgcgcccag cgaacgcctt
    15961 ccatactcaa tgcgacggtc gacccgtccg ccagcatcgc cgtcgctccc tgaggattgg
    16021 cgctggtgac tgcggcaggc agcagcggac cataggtcgg cagcgccttc agcgtatcgg
    16081 taatcttgtt gttatcccac gccgactcgc ccactttcca cagcacattt gccgggccgc
    16141 gatagccgtg gcgcatgtcg tagtccagca cgttattacg taccgcctgc tgcgcggcct
    16201 gctgcacttt gtgggtgatg gtggtgtaaa tgcgataacc gtcttcatag gcactttcgc
    16261 cataacggtt atacatctcc tggcgcacca tttcgctcag gtacggcgca gagaaagcaa
    16321 tctccggcgc gtgataatta gcgttaatcg cctcagtgcg tgtctgatcg aactgttgct
    16381 gggtgatata cccttcatcc agcatccgcg acagtacgac gttacgccgc gcgacggcac
    16441 gatccattga gtagagcgga ttaaaggtgg aaggcgcttt cggcaacccg gctatcaccg
    16501 ccatttcgtt cagcgtcagt tggtcgaccg ttttaccgaa atagacttgt gccgcagcac
    16561 cgacaccata ggcgcggtaa ccaaggtaaa tcttgttcag ataaagctcg aggatttcgt
    16621 ctttcgtcag caactgttca atgcgaatcg cgaggaagac ttccttaatc ttacgcatca
    16681 gcgtgcgttc tggactgagg aagaaatttc tcgccagctg ctgggtaatg gtactcgccc
    16741 cttgtgacgc gtggccggag aacagcgcca cacttgccgc acggaagatc cccaccgggt
    16801 caacgccgtg atgctcgtag aagcggctgt cttccgtcgc gataaaggct ttcaccatct
    16861 ccggtgggat ttgatccaac gtaaccggaa tacgacgttt ctcaccgtat tgagcaatca
    16921 gctcgccatc ggcgctgtaa atctgcatcg gaatttgcag gcgaacatct tttaatgtcg
    16981 ccacatccgg cagttgtggc tcgatgtagc ggtataggcc ataaatcgag cctgctccca
    17041 gcagaatgca acagactgca aggatcaaaa aatactttac gaacttcact ggaaatttcc
    17101 catttagttt catttgggca gtttataaac aaacgcgcgg tagtataaag gcaagccagg
    17161 cgcattgata tacccgtcag agttacgggt gataaggaga tcatcacaat ggcatttaag
    17221 atctggcaaa ttggtttgca tttacaacag caagaagcgg cagcggttgc gatcgtgcgg
    17281 ggcgcaaaag aatgcttttt gcaacgctgg tggcggttgc cgctggcgca cgacattatc
    17341 aaagatgggc ggattgttga tgcgcagcag ctggctaaaa cgttgttacc gtggagtcgc
    17401 gaactgccgc agcgtcatca cattatgttg gcgtttcccg ccagtcgcac attacagcgg
    17461 tcatttccgc gcccgtcgat gtcccttggt gagcgggagc aaacggcttg gctgtcaggg
    17521 acgatggccc gcgagctgga tatggatccg gactccctgc gcttcgatta cagcgaagac
    17581 tcactcagcc ccgcttataa cgtgactgcc gcgcaaagca aagagctggc aacgctgctt
    17641 acgctggcag aaaggttgcg tgttcatgtt agtgcgatca ccccggatgc cagtgcatta
    17701 cagcgattcc tgcctttttt accttctcat cagcaatgtc tggcctggcg tgataacgaa
    17761 cagtggctgt gggcgacgcg ctatagctgg gggcgcaaac tggcggtggg gatgactagc
    17821 gcgaaggagc tggcggcagc gttatccgtt gatcccgaca gcgtcgcgat atgtggcgaa
    17881 ggcggatttg acccctggga ggccgtttct gttcgtcagc cgccgctacc gccgcctggt
    17941 ggagactttg ccatcgcgct ggggctggcg cttgggaagg cgtactgatg aacccgccaa
    18001 ttaatttttt gccctggcga cagcaacgcc ggaccgcttt tctgcgtttc tggttgctga
    18061 tgttcgttgc gcctctgctg ctggccgtcg ggataacgct aatactgcgt ctgacaggca
    18121 gcgccgaagc gcgcatagac gcagttttgc ttcaggcgga acaacaactc gcccgcagct
    18181 tacagataac gaagccacgt ttgctggagc aacagcagtt acgcgaacag cgttcgcaac
    18241 ggcagcgcca gcgacaattt acccgcgact ggcaatctgc gctggaagca ctggcggcgc
    18301 ttttacccga gcacgcctgg ctgacaacga taagctggca gcagggaacg ctggagatca
    18361 aggggctgac aacaagcatt accgcgttaa acgcactaga aacgtcactc cgccaggatg
    18421 cgtcttttca tctcaatcag cggggagcca cgcagcagga tgcgcaggga cgctggcaat
    18481 ttgagtatca gttaacaagg aaggttagcg atgaacatgt tctttgactg gtggttcgcc
    18541 acatcacccc gcctccgcca gttttgctgg gcattctggt tgctgatgtt agttacgctc
    18601 atttttctat catcgacaca ccatgaagag cgcgacgcat taattcgact acgggcaagt
    18661 catcaccagc agtgggccgc actgtatcgc ctggtagaca ccactccctt cagcgaggaa
    18721 aaaacgctgc ccttttcgcc actggatttt cagttatccg gcgcgcaact ggtttcctgg
    18781 catccatccg cgcagggagg cgagttggcg ttgaaaacgc tgtgggaagc agtgccgtcg
    18841 gcatttacac ggctggcaga gcgcaacgtc agcgtgagcc gtttttcgtt aagcgtggaa
    18901 ggtgatgatc ttttgttcac gctacaactg gagacgccgc atgagggtta aacgctggtt
    18961 gttggcaggt attgcattgt gccttttaac cggtatgcgt gaccctttta aaccgccgga
    19021 agatctatgc cggattagcg aacttagcca gtggcgctat caggggatgg tagggcgagg
    19081 cgagcgcatc atcggtgtaa taaaagacgg gcaaaagaaa tggcgacggg tgcagcaaaa
    19141 ctatgtgctg gaaaacggct ggacaatttt acagctgacg ccagacgtac taacgctggg
    19201 taccgggaca aactgcgaac cgccacaatg gttgtggcaa cggcaaggag atacaaatga
    19261 agcaatggat agccgcacta ctgttgatgc tgatacccgg cgtacaggcg gcaaagccgc
    19321 aaaaagtgac gctgatggtg gatgacgttc cggtagctca ggtgttgcag gcgctggctg
    19381 aacaggagaa gttgaacctg gtcgtgtcgc cagacgtcag cggtacggtg tcgttacatc
    19441 taacagatgt tccctggaag caggcactac aaactgtagt gaaaagcgcc ggactgataa
    19501 cgcggcagga aggcaacatt ctctcagtgc attccattgc ctggcagaat gacaatatcg
    19561 cccgccaaga ggcggagcag gcgcgggcgc aggcaaatct gccgctggaa aatcgcagta
    19621 taaccctgca atacgccgac gcgggagaac tggcgaaagc gggggagaag ctactgagtg
    19681 ccaaagggag tatgaccgtc gataaacgca ccaatcgcct tttgctacga gataacaaaa
    19741 cggcgttaag cgcgcttgaa cagtgggtag cgcaaatgga tctgccggtc gggcaggttg
    19801 agctgtcggc gcatattgtc accattaatg aaaaaagttt gcgtgattta ggcgtgaaat
    19861 ggacgctggc cgatgcgcaa cacgctggtg gcgttgggca agtcaccacg cttggtagcg
    19921 acctctccgt agcgacggcg acaacgcatg tcggttttaa cattgggcgc atcaacggac
    19981 gcttgctgga tcttgagctt tccgcgctcg aacaaaaaca gcagctggat attatcgcca
    20041 gtccgcgtct gctggcctca catcttcagc ctgccagcat taaacagggg agcgaaattc
    20101 catatcaggt ttccagcggg gaaagtggcg cgacgtcggt ggaatttaaa gaggccgtcc
    20161 tggggatgga ggtcacgccc acggtgttac aaaaaggtcg catccggctg aaattacaca
    20221 tcagccagaa cgttccgggg caggtgctac agcaggccga tggcgaagtg ctggcgattg
    20281 ataagcagga gatcgaaacg caggtcgagg taaaaagcgg agaaacgttg gcgctgggcg
    20341 gcatttttac ccgtaaaaat aaatcgggtc agga
//
LOCUS       DADLSA010000069        19970 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.069, whole genome shotgun sequence.
ACCESSION   DADLSA010000069 DADLSA010000000
VERSION     DADLSA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.069"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..758
                     /locus_tag="JN691_002414"
     CDS             <1..758
                     /locus_tag="JN691_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAY7243836.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSEAQHYDFIKKKSIYEKLNSKSSFD
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
     gene            complement(827..1783)
                     /gene="yjiA"
                     /locus_tag="JN691_002415"
     CDS             complement(827..1783)
                     /gene="yjiA"
                     /locus_tag="JN691_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAY7243837.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQMGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(1794..1997)
                     /locus_tag="JN691_002416"
     CDS             complement(1794..1997)
                     /locus_tag="JN691_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY7243838.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2047..4197)
                     /gene="btsT"
                     /locus_tag="JN691_002417"
     CDS             complement(2047..4197)
                     /gene="btsT"
                     /locus_tag="JN691_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAY7243839.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            complement(4590..5102)
                     /locus_tag="JN691_002418"
     CDS             complement(4590..5102)
                     /locus_tag="JN691_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HAY7243840.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            complement(5120..6682)
                     /gene="hpaB"
                     /locus_tag="JN691_002419"
     CDS             complement(5120..6682)
                     /gene="hpaB"
                     /locus_tag="JN691_002419"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HAY7243841.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            complement(6909..7765)
                     /locus_tag="JN691_002420"
                     /pseudo
     CDS             complement(6909..7765)
                     /locus_tag="JN691_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376701.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(7873..8763)
                     /gene="hpaA"
                     /locus_tag="JN691_002421"
     CDS             complement(7873..8763)
                     /gene="hpaA"
                     /locus_tag="JN691_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HAY7243842.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            complement(8773..10128)
                     /gene="hpaX"
                     /locus_tag="JN691_002422"
     CDS             complement(8773..10128)
                     /gene="hpaX"
                     /locus_tag="JN691_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HAY7243843.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMAIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            complement(10150..10893)
                     /locus_tag="JN691_002423"
     CDS             complement(10150..10893)
                     /locus_tag="JN691_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HAY7243844.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGLADLSADMGYAGNPQHPEVQAANEQLAKRYLELGALFVAVGVDTTL
                     LARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            complement(10904..11707)
                     /gene="hpaH"
                     /locus_tag="JN691_002424"
     CDS             complement(10904..11707)
                     /gene="hpaH"
                     /locus_tag="JN691_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HAY7243845.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            complement(11775..12155)
                     /locus_tag="JN691_002425"
     CDS             complement(11775..12155)
                     /locus_tag="JN691_002425"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HAY7243846.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMEGRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            complement(12165..13016)
                     /gene="hpaD"
                     /locus_tag="JN691_002426"
     CDS             complement(12165..13016)
                     /gene="hpaD"
                     /locus_tag="JN691_002426"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HAY7243847.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            complement(13018..14484)
                     /gene="hpaE"
                     /locus_tag="JN691_002427"
     CDS             complement(13018..14484)
                     /gene="hpaE"
                     /locus_tag="JN691_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HAY7243848.1"
                     /translation="MKKVNHWINGKNVADNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTTTGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            complement(14481..15770)
                     /locus_tag="JN691_002428"
     CDS             complement(14481..15770)
                     /locus_tag="JN691_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HAY7243849.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            16042..16488
                     /gene="hpaR"
                     /locus_tag="JN691_002429"
     CDS             16042..16488
                     /gene="hpaR"
                     /locus_tag="JN691_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HAY7243850.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAEST
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            16607..18271
                     /gene="tsr"
                     /locus_tag="JN691_002430"
     CDS             16607..18271
                     /gene="tsr"
                     /locus_tag="JN691_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY7243851.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPCQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(18320..19681)
                     /locus_tag="JN691_002431"
     CDS             complement(18320..19681)
                     /locus_tag="JN691_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7243852.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSIRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREKLGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
BASE COUNT         4846 a         5087 c         5247 g         4790 t
ORIGIN      
        1 attatactaa gattttagag aatgattttg aacacccttc aatacgacga aaaatagcat
       61 cgctaagtaa tttaggggta gatgagtgga ttcctccggt tatggcattc atgaatcgaa
      121 tggctcgtac agaagatttt aatttagatg atttttctca atttattacc gcctttgaga
      181 aggtatatat gcatgggtgg ttaaaaaaac aaattaaaag ccaaagagaa atggtttgtt
      241 attcagctct ggtagcaata aataatgata tgccttttga ttctgtgata aatcagataa
      301 accaacatgc agataattca ggttttatag ctgctctgga tgaagattta tatgagcctc
      361 gtccgaatca agtgaactta ataaaagcaa ttttgcttcg cttagatatg gagcaacaag
      421 atgagagcgt gattaaaacc tatacgggga gaatcacaat tgagcatatt cttcctcaag
      481 cattagttaa tgaatactgg attaatagat tccaacctca agagcatgtc tactggctgc
      541 ataaaatagg taatttaaca ctcataagtg gttccaaaaa ttctgaagca caacactatg
      601 attttatcaa aaagaaatct atttatgaga aacttaatag taaaagctct tttgatttaa
      661 caaaagatgt ttgtaattct tcagaatggg gattggctga actgaaaatg agacatgaaa
      721 aaatgaaaac gcaattgaag aagctttggt tggtctgaca tatctaaaga gttccactcg
      781 taataatgtt gcggtggaac tctcaaaaag ttgatctttc ccgaacttac ttcctcaatc
      841 ccgcaaacgc cgcccgaatc tcttcctccg gcaactgaat cccgataaac accattgtgc
      901 tatgcggttt ttcatcgccc cacggcctgt cccagtcggc gctgtagagg cgctggacgc
      961 cctggaacag caggcggtta ggttcgccgt caatccacag catccctttg taacgcagca
     1021 gtttatccgc cgattccagc agcaggtttt ccatcacgcg ggaaacttcg ctgatatcta
     1081 ccgggtaatc cagttccacc acaattgacg aaatatcgtt ttgtttatcc gcgataaagt
     1141 ggaaacgcgg tttggtgctg acgacgttct cttccagcat aaaaccgttg gtgttgaaca
     1201 gcaggcccag gtcgatgtcg ccgtgggtga cggtgtagac cggtgcgcgg gcgttgatgc
     1261 gcgccaggcg ttcacgcagt ttctctgctt cgcctgcgac gtcggtttta gtcagcagaa
     1321 tacggtcggc gtagcccatc tgggactggg cgatggtgaa ctggttcatc tgctcatcgg
     1381 catgtaccgc atccaccagc gcaatcacgc cgtccagcag gtagcgctgg cataaaatct
     1441 catgggagaa aaaggtctga ataatcgggc cgggatcggc catgccggtg cattcaatca
     1501 ccagacgatc gaactgaata ttgcccttgt cgagattatc cagcaggtcg agtagtgcgt
     1561 cctccagctc gttggagcgc gaacagcaga tgcagccgtt ggtcagcgtt ttgatctgcg
     1621 tggcgcggtc gccaatcagt tgatcatcaa cggagacttc gccgaattcg ttttcaatca
     1681 cggcaatctt gtagccgtgt tgttcgttaa gaatatggcg cagcagggta gttttgcccg
     1741 cgcctaaaaa accggtgagt agggtaactg caatcgggtt catgctctct ccattaacaa
     1801 cagcgcatac cgcctttacc atcgccgccg tagcgcgcgt tctggcgttc gcggaagaat
     1861 tcttcatagc tcatgtacgg cttgtcggga tggttggtct tcatatgctc gacgtagttg
     1921 tcgtagtctg gaataccaat caacatcttc gccgcctggc cgagatattt ttttgcctgt
     1981 cctaagttac caaacatagt tcactctgat aagaacaaag ccccgccgaa gcggggctaa
     2041 acacggttag tggtgcgaag agatcttcac gccgccttcc gggattggaa cgtacggtgt
     2101 ttctttgtca gtacgtttgt cgctgttacg caccgcaagc caggttttga aaccgtagaa
     2161 gatgatgctg tacaccacaa tcaggaacag aatactcagg cctgcgttgg tgtagttgtt
     2221 cacaacgatg tggttcatgt tggcaatctg ctgcgccgtc aggtcagtac cgttagcaat
     2281 cttctctttg tactggcttg ccatgtagaa gaagccttcc atctgcgggt tggtgctgaa
     2341 cagtttcaga cccagcgccc aggtggtgca gataagcagc catacagccg gaacaacagt
     2401 tacccagatg tattgggtgc gcttcatctt aatcagcaca acggtgccca gtaccagcgc
     2461 tacggctgcc agcatctggt tggagatacc gaacagcggc cacaggctct taacgccgcc
     2521 cagcggatcg accacgccct gatacagcag gtagccccac agacccacac agcccgcagt
     2581 accgatgata ccggcaacca gagagtcggt ttttttcagg aacgggatga agttacccag
     2641 caggtcttgc agcataaagc ggccagaacg ggtacccgca tccagcgcgg tcaggatgaa
     2701 cagggcttcg aacagaatac cgaagtgata ccagaagccc atgtcagcca tcggcagcac
     2761 tttgtggaac acgtgagcga tacctaccgc cagcgtcggc gcaccacctg cacggttcag
     2821 gacagaaggc tcaccaatgt ctttcgcggt ttgcaggatc tgctctggcg aaatcacgaa
     2881 gccccaggag ctgacggtcg ctgccgcgtg tgcggtaacg tctttcagct gcgccatgat
     2941 gatcggcgcg ttctcgccac ccatttcatg caggttaggc atggtgatgc caaggccagc
     3001 aggcggggtg ttcatcgcga agtaaagacc cggttcgatg atggacgctg caaccagcgc
     3061 cataatcgcc acgaaggact ccatcagcat tgcgccgtag ccaatgaaac gcgcgtcggt
     3121 ttcgttagcc agcagtttcg gcgtcgtacc ggaagagatc agcgcgtgga agccagatac
     3181 cgcaccacag gcgatggtga tgaacaggaa cgggaacaga gcgcctttcc acagcgggcc
     3241 agtaccgtca atgtactggg tcatcgcagg cattttcagt tccgggttca gcaccacgat
     3301 acccagcgcc aggccgacga taacgccgat tttcaggaag gttgccagat agtcgcgcgg
     3361 tgcgaggatc agccacaccg gcagcagtgc ggaaacaaac gcatagccaa tcagcgcgaa
     3421 ggtaatggtg gtgtctttaa aggtcagtgc cggaccccag tacggatcgt gagcaatcac
     3481 gccaccgaag tagatagagg caaccagcag cacgatacca ataacagaga cttcacccac
     3541 acgccccgga cggataaagc gcatgtagat acccataaac agcgcaatcg gtacggttga
     3601 gcaaacggtg aagacacccc acggactttc ggccagggct ttaaccacaa tcagcgccag
     3661 aacggcgagg atgatgatca tgattaagaa acagccaaac agcgcgatag tccccggtac
     3721 tggccccatc tcttctttga tcatctcacc gagagatgcg ccgttacggc gagaggagat
     3781 aaacagcacc ataaagtcct gtaccgcacc ggccagcact acccccgcca gcagccacag
     3841 cgtgccaggc aggtagccca tctgcgcggc gagaaccggt cccaccagcg gaccagcacc
     3901 ggcgatagcg gcgaagtggt gaccaaacaa cacgtaacgg ttggtcggaa cgtagttcag
     3961 accgtcgttg ttaataaccg caggcgtcgc gcgcgtgggg tcgagtttca tcaccttctg
     4021 ggcgatgtac agactgtagt agcgatacgc caccagatac accgacacag aggcgaccac
     4081 gatccacagg gcgctgacgt gctccccccg acgtaatgca actaccgcga ggcagaatgc
     4141 accgatgatt ccgagaatca cccagggtat gtgcttgaat agttttttcg tatccatagt
     4201 aaaacctggc attttactcg tcgtcttttg gcttacaggt gcggcagctg cgttcgttca
     4261 cctctgacac ttacttttgt aagctcccgg ggattcactc acttgctgcc ttcctgcaaa
     4321 ccaaaatcca tagagtaatt tgttaatcat aggccgaagc cgttctaggt ttgcgttgcg
     4381 tttgaggagg taaattgacc gctatcctgc agtgatgttg ccagagatac gtgcgcgtaa
     4441 agtaaggtaa ataactgagt ggttatttta gggatgtaag cggtcaggaa tggcggtgag
     4501 cggttgggca agaattattc atcgggtaga tatgtgattg agatcacaat aaaaatgccc
     4561 cttcgacgaa tgcgtgaagg ggctggagct taaatcgcag cttccatttc cagcatcacc
     4621 ggatggaaac ggcgtttaaa gtaaataagt ccgtgacctt ctgcactgag gatgatgttt
     4681 ttaatctcca ccagatacac cagatgtgtg ccaatcgcct gcacatcgcg gatctctcct
     4741 tcaagactgg ccagcgaacc ttttagcacc ggctgcgcca gcggaccttt ttgccagcat
     4801 gagaggctaa aacgctcttc catcgccatg cctgtcatgc ccgcgaagtg gcgcgccatc
     4861 agttcctgct catggttgag gacgttgacg cacaacttgc cgttgccctg aaaaaccggg
     4921 ttcatcgcac tgttggcgtt aatgcacacc atcagcgacg gtggtgtatc cgtgaccgag
     4981 cagacagccg ttgccgtaat cccgcattgt ccggcgtcgc cctcggtggt gataatattt
     5041 accgctgccg acaggctggc catcgcgtca tgaaagcgca ggcgttgttc atctaattgc
     5101 atcttaacct cctgctgcgt tatttcagca gcttatccag catgttgata tcgtcgttgt
     5161 tgtgcaggtg cggcacagtc cagccgttct ggtcgtattc cgacaggcag cgatcaacca
     5221 tcgccatcat cttgtccata ttgccggagc tttgtgcctg gcgcagacat tgcaggcgaa
     5281 tctcatcctg gctaccggag tagttgattt catacagctc gtgacgacca ccaaattcgc
     5341 tgccaatggc gtcccacatc agtttgagga tcttgatgcg ctggacgtga tccataccgt
     5401 tcgaaccgcg cacatacttc gccagatact ggtcgatctg cggattgttc aggtcacggg
     5461 cactggaagg gaggtagatc aggccactgg taacgttgcg ttctataatg tttttgatct
     5521 tcgcgtaggc cattggtgcc agtacgcgat aggtttgcag tgcggcatga tccggtaaat
     5581 aagccccgtt gacccacggc gtcgcttctg aacacatcga gtcactcaat gcccagaagg
     5641 tgttgcgcca cgccaccact tcaccgagat cggcctgcac accacggaac tccagggtgc
     5701 cggtacattc gagtgatttt ttcagcagtg ccgtaatgaa gtcgagtttc actgccaggc
     5761 gcacacaggc ttgcagcgga tacatacggg cgaaaccgcc ttccatcgtc cagcgacggc
     5821 agcgatcaaa atcgcggtag atcagcacgt tttcccatgg gatcagtacg ttatccatca
     5881 ccagaatcgc atcgttctca tcgaagcggc tggagagcgg gtagtcgtat ggcgagccgg
     5941 tagcacccgc gaccatctca taagaggcgc gggagattaa tttcacgcca tcggcatcca
     6001 ttggcgcaac gaacatcagt gcgaagtccg ggttttcgcc catcacttgt gccgagccga
     6061 agccaatcat gttgtagtga gtcagcgccg agttggtggc aaccactttc gcaccgctga
     6121 cgataatccc ggcgtcagtc tctttttcca gcttgatgta aacgtctttc actttatcgg
     6181 tcggcaaatg acgatcgatc ggtgggttaa caatcgcgtg gttaaagtag aggccagttt
     6241 cctgaatacg ggtataccag ttacgggcgt tctgctcgaa ctgaccgtaa aagcccggat
     6301 tcgcgcccag tgcgcaaccg aaagcggctt tgtagtctgg ggtacggccc atccagccat
     6361 agctcaggcg tgaccattca gcgatggcgt cgcgttgctg gcgcaggtcg tcggcacttt
     6421 tcgccacgcg gaagaattta tgggtatagc cgccgctgcc ggtgtcggtg ttccaacaca
     6481 gagagtcctg catctccggt ttgtgcagcg cgtcgtacag ttgggcaaca gacgctgccg
     6541 cattacgaaa tgccggatga gtggtgacgt ctttcactcg ctcgccatag atatagatct
     6601 cgcgaccatc ctgcaggctt ttcagatact cttccccggt gaaaggacgt tgggtactgg
     6661 cgcggaaatc ttctggtttc atgtcgacct ctacagtatt aatcggaata cgattgttaa
     6721 ttttgtgtgt tgtttttgtt atttaattga agcgttgaac gcggttgtcg ggaagagacg
     6781 tttcaggcga acgttggtac ttttcgacag caaggtggag ttaaatagta aactgcgatc
     6841 gtgatcaact tacagtaagg caaatcataa tattccccgc taacaagggc taacggggac
     6901 ggaatttttt atggggcgaa tggaatggta atcgtcatat tttcatgacc aacgacgata
     6961 gggcctgaat agttttctgt gcttcttgct tccatgtttc atccggaacg gtgattggta
     7021 ccaggtggct aaggatgagt tttttcactt ttgcttcatt ggcaattttg cctacatcct
     7081 caatagtcgt atggtgagaa gcaatggctt ttgccagtgt tttaccattg ccaatactgt
     7141 ctgccagctt agcgacggag ggctcatgta cggcctcatg cacaagtata tcagcgcctt
     7201 ctgagaaagt tgccagtggg ggaaagtacg aggtatctcc tgagaagaca attcgcttgc
     7261 cttgtgtgtc gaagcgataa gcaaaagcct caccgtcagg gaaaggagaa tgagggacct
     7321 ttaatgcagt cacggtcacg gtatcatctt tataaactac gccttcacca acatcttgag
     7381 ctgtaacgag tttgcgagga tcgggttttc cttcttcttc catacgcagt gtgatgtccc
     7441 gatcgaagac ctgccaactg gcagcggtca tggctcgggt gcctggcgga ccgaatacat
     7501 gaatagtttt gtcctgcaga ccgctagccc atgcattcat taagactgat ggatagtcca
     7561 gaatatgatc actgtgtaaa tgggtgagta aaatattatc aatctggcgc agtggaatat
     7621 tattttttac cagttgtaag ctggccccgt aaccagcatc aattaactag gttttattgc
     7681 caataataag tgccgttgcc atcggtaaac ggtcagcttt taataaagaa gggccacctt
     7741 tcgttccaag tagtacaatt ttcatttatt gcggttcgga ggcgtaggtg aatgccactg
     7801 gcgcaatcag gcacgccaca aatagtgagc gtaaagatat tttcatgtcg agtctctcag
     7861 gttgataaat ggtcagcgac cacggaattg tgttggtgag caacccatca ttcggttgaa
     7921 aaaacgggcg aaataggcgg gatctttgta gccaagatca agggctatct ggttgatgct
     7981 ttgctcgcta tagagcagaa gccgtctggc ttcccggctc actcgttcga aaatcaatct
     8041 cttcggcgat tgattggcaa aacgtcggca cagctcggta agccgtgatt cactcatacc
     8101 gagcattgtt gcatattcag ggaccattaa atgttggcga taattttcgt caatcagccg
     8161 attaaacttc tggaataaac acatctcgcc gcgcacgctg catgttgaaa tatcatttgg
     8221 tggtgcttca cgcagtagga ttgtaaaaat actttgcgcc agtgattgca atagaatttc
     8281 ccgtcctggc ttattttgtt gaaactccag ggatattatc ggccaataat gatttaatgc
     8341 cgccagtgta tgttccattc cctccagtga cagacagata ccttgtagca ttgcggcatc
     8401 tccactacca ggccagagct tttctaccag gggccaaatt aactcctgac gtacggtcag
     8461 aacatgccca tcgctatcag gatcagtaaa gaaagtatgt ggcaccgatg gtggtgttag
     8521 tacaaaaagc ggtgcgcgca cagaatagaa gtgctcatcc agttgtaaag taatgcgtcc
     8581 agtgaccaga taatggagtt gaaaatatct atcgtgccag tgagggcgca tatcgcgtcc
     8641 aaagaaattc gctaaccggg caaatgtttc ataatgcaca tcttcaccag cgtagcgggc
     8701 atcatattcc cggaccaaat caatattttc ataaccatgt tcaaagatag cttcactgga
     8761 catcatgact ccttattgcg gttttggtgc gataagttca gatgaaagag agtgttcccg
     8821 actccccata gggattcggg tcatgactaa cgcgcccagt accagcaatc ctgctacaaa
     8881 ccacaaacct gagctaaaac tgccggttgc atctttcaaa ataccgataa gtaatggact
     8941 gactgctgaa cctacattgc cgacagcatt gatgacagcc atcgcaacgg cacgcgattg
     9001 caagcttata acttgatccg gtgttgtcca aaagatagcc atcgcggtaa atgatcccat
     9061 tgaggccata atgatgccgc tcagttgaat gaggctgtga tctgtcgcag aagcgagtaa
     9121 ccatccggca gcggcaaaca ggtagggaag aatcgtatgc tttttgcgtt ctttaagacg
     9181 gtcggaacga cgactccacc agatcatgcc aaaaattgtg cagaattggg ggatggccgc
     9241 cagcaaacca atcatgatgt tgctactgcc agtattgaaa ctctgtagga tttgcggcgt
     9301 ccagatatta atcgcactca gagtattggt caggcaaaaa taagcaagtg tatatagcat
     9361 gatggctggg gtcaggactt cacgtaaggt tgagcgtgga acagatgcat gggcaaatgc
     9421 actctcttgt tcacggagaa tcatcgtttt taaagcctgt ttttcgtttt catcaagcca
     9481 ggtcgcctgg tctggtgtgt cgttgagata aaaccaggtg acgacaccaa gaatgaccga
     9541 tggcagacct tccagcaaaa agagccattg ccagcctttt agattccaca gtccatccaa
     9601 ggccagaatg tagcctgaaa ggatcaatcc cagcatcata gtgactggca tcgctatcat
     9661 aaataaggca ttggctctgg cccgatgata tgccgggaac caccaggtca gataaaccaa
     9721 aataccaggt aaaaaacccg cctcagcaat accaaccagc atacgcagta gatagagtgt
     9781 ttcggggctt gttgcaaaca ttgtgcaggt ggaagcaatt ccccagacca ccatgatccc
     9841 agcgatccat cgacgagcgc caattttcgc taacatcata ttactgggga tcccgcacat
     9901 gacgtacgtg acataaaaga gtgtcgcggc gaggccaaac attgtggacg tcaggccaag
     9961 atccttgccc attgtcagtc cggcaaatcc tatattgatg cgatcaagaa acgaaaaaac
    10021 aaacagaata aaaagaaaaa tgattaggcg acgaaataat ttattaatga cacgatgttc
    10081 aatttcaaca gttttattat ctttatattg cagggtagag gtcgtcatga tgcgctccac
    10141 atcgtacgtt tagtagacag agttgttgtt ttgcttcgcc atcgaaatag aagaactatt
    10201 accaaaccgt gctgccagcg cttcggcggc gcgggcgagc agggtggtgt caacgccgac
    10261 ggcgacaaac agcgcgccca gttccagata gcgttttgcc agttgctcat tggcggcctg
    10321 tacttccggg tgctgcggat taccggcata acccatatcg gcgctcagat ccgccaggcc
    10381 gataaacacg ccgtcgaccc cttccacgtc cagaatctgc ggtaagttct tcattgcctc
    10441 acgcgtttcg atctgcacca gcacgcacat ttgatcgttg gctttttgca ggtaatcagg
    10501 aatgcgattc cagcgcgagg cgcgagccag cgcactgccc acaccgcgaa taccggcggg
    10561 gggataacgg gtggcgcgta ccgctttacg ggcttcgtcg gcgttttgta ccatcggcac
    10621 cagtaaggtt tgtgtgccta cgtccagcag ttgtttgatt tgcaccggat cgttccacga
    10681 tgggcgtacc actggctggc tggggtaggg cgcaatcgcc tgtagttggg tgagcacggt
    10741 ttgcacgtta ttcggcgcgt gctcaccgtc gatcaataac cagtcgaatc ctgctccggc
    10801 cagtaactcc gcgctgtagc tgctgctcag ccccagccat aatccaatct gcggacggcc
    10861 tgctttcagc gccgctttaa aactgttttc catcgttctc tccttaaaca aagcggcagc
    10921 taatggagcc catgttgccg taatcgacgt ggaaggtgtc gcccttacgc gccggaaccg
    10981 ggcgggtgaa cgaaccaccg agaatgattt gcccggcttc cagttgtacg tcatacggtg
    11041 cgagtttgtt cgccagccag gccacgccgt ttgccggatg attcagcaca ccagcggcga
    11101 cgccggtttc ttcaatcacg ccattgcgat acatcagggc ggagatccaa cgcagatcca
    11161 gttcgtctgg tttgatcgga cgacctccga ggatcactcc ggcgttggcg gcgttatcgg
    11221 aaatggtgtc gaatactttg cgcgggcgct gggtttccgg atcgatgttg tggcagcgag
    11281 cgtcgatcag ctccagcgcc gggatcacat agtccgtggc gttgtaaacg tcgaacagcg
    11341 tgcagtttgg cccacgcagc ggttttgcca gcacaaaagc cagctccact tcaatgcgcg
    11401 gcacgataaa gcgatcggtc gggatatcgc tgccatcgtg gaagaacatg tcgtccagca
    11461 gtgcaccgta atccggttcg ctgatctgcg agcttgcctg catcgctttc gaggtcagac
    11521 cgattttgtg gcctttcagc gtgcgacctt cggcgatttt caggcgaacc cattcgcgct
    11581 gcacggcgta ggcgtcttcg atggtgatct ccgggtaatc cagcgagatc gcgcggatct
    11641 gttcgcgctg tttttctgcc tgatccagac gctgggcgat cagggtgtgg gtgtgtttat
    11701 cgaacatagc gatatcctgt ggcattgccg gatgcggcag cgccttatca tccggcctgg
    11761 aagctggtgc aaatttattt aaacaacgcg tgtacgttgt tctgtttgaa attcaacgtc
    11821 gggtgcagct ctgcaatctc aaatgaaagc gctaggtaac ggccctccat cagtgaggcg
    11881 aagtgcgttt taatcagatc aaacagcatt tcgcccactt cctgacggct ttccagactg
    11941 cgtccgctgc cgatttttag cgtcatatgc acaaaggcgt aatcgtgctt gccatcagcc
    12001 atttgccagg tatcgatcca gtgcgcccgg ctacgtattc ccccaagcgg aaaaatcccc
    12061 gttgccgcca gtgcagtgtt aacactggca aacagctcag gtaagcgggc ttcttcacga
    12121 atattttcag tacattccac gataaagtgc ggcataaaac gtccttacgc gggcagcggg
    12181 aaaacagcgt taacctgacc ggtgccagag cttgggaaca gctcggtaat aaactccacc
    12241 ttgccgtcgt atttatccca gccgagcatc cccagcagca tcaccgtgtc gtgcatattg
    12301 ccttcgccgt agcagtagtc ggcgtactcc ggcagcatat tgcaaaactc tttgaactgg
    12361 ccttcgcgcc acagcttcac cacacgctcg tccatctggc ggtcgaactc gcgggtgtag
    12421 ctgttcatcc cttcttccgc acgctgatcg tcaataaagc ggtgcgataa cgaaccgctg
    12481 gcaaggaccg ccacggtgcc gtcgtactgt tcgatcgctt tcagaatcgc ttcgcccagc
    12541 ttgcggctgt cggcaaaatc gtgaaccgtg cagaaagccg aaatggagac cactttgaag
    12601 tgcttgtctt cattcatgta gcgcatcggt accagcgtgc cgtattccag tttcaggctg
    12661 ggaatattgt gcgcttttgc ccgcacgccg agctttaagg cttcctcggc aattagctgc
    12721 cccaactccg ggttgccctc gtagttgtag gtcatgtcgc gaataaaatg cggcagctcg
    12781 ttgctggtgt agacgccttc aaaatggtct gcacagttga tgtgataagc actgttgacc
    12841 agccagtgag tatcgaaaac gataatggta tcgacgccca tttcccggca acgcttgctg
    12901 atctctttat gcccgtcgat cgcaccctgg cgacaaccgt ggttttttcc tggtagttca
    12961 gagagataca tcgacggtac gtgcgtgatc ttggcggcta acgctaactt acccatatca
    13021 gactccccat ttcggaattg gatggtcgcc catggaaatg cagacgttct tcatttccgc
    13081 gaacacttcg aagctgtact caccgccttc acgcccggtg ccggaggctt ttacgccgcc
    13141 aaatggctgg cgcaggtcgc gcacgttctg ggtgttgacg aacaccatgc ctgcttcaat
    13201 gccacgcgcc aggcgtaaca ctttgctgac atcctgtgtc cagatgtacg acgcgaggcc
    13261 gtactccacg tcgtttgcca ggcgtaagcc ttcggcttcg tctttaaacg gcagcaggca
    13321 ggcgaccggc ccgaaaatct cttcctgggc aacgcgcata cggttatcta cgtccgccag
    13381 caccgttggg cgcaggaagt tgccgccttt caggtgcgca ggcaggtcgg acggtttatc
    13441 cgggccgccc gccaacaggg ttgcgccttc ttcaatgccg agacggatat agccggagac
    13501 tttttcccag tgttgctgac tgataagcgc cctaacctgg gtattcggat cgttcggatc
    13561 gcccacgcgc agacggttgg cgcgttcggc aaagcgttta acgaattctg gatagatgct
    13621 ctgttgaata aagatgcgcg aaccggcggt gcagcgttcg ccgttgattg agaagatggt
    13681 gaacagggcg gcgtccagcg cgcgctcaat atcggcatct tcaaaaatca gcaccggtga
    13741 tttaccgccc agctccatgg agtatttttt cagcccggcg tttttcatga tgttgcgccc
    13801 ggtggtggta ccgccggtga acgacacggc acgcacgtca tgatgacgga ccagcgcatc
    13861 gcctgcggtt gcgccgtagc cctgtaccac gtttagcacg cccgccggaa taccagcttc
    13921 cagcgccagc tcacccaggc ggtcggcggt cagcggggag agttccgaca tcttcagcac
    13981 cgcggtattg cccagcgcca gacacggcgc gaccttccag gtggcggtca taaacggcac
    14041 gttccacggc gacaccagcg cacaaacgcc taccggttgc accagcgtgt agttgagcat
    14101 cttgtcgtca accggatagg tcttgccgtt catctgctgg cagacttccg cgaaaaattc
    14161 aaagttgtga gaagcgcgtg ggatcaacac atttttggtc tgatggatcg gcaggcccgt
    14221 gtccgcggtt tccatcgcgg cgatctcagg cacgttctgg tcgatcagat cgcccagacg
    14281 gcgcatcagg cgcgcacgct ctttcatcgg cagatcagcc catttcggga acgcctcttt
    14341 cgctgccgct accgcctgat tgatctccgc ttcaccgcca gaggccacat ccgccagcac
    14401 ttcacccgtt gccggattgg tagtctggaa gtagtcgtta tctgcaacat ttttgccgtt
    14461 gatccaatga tttacttttt tcatttcgct gtttcctcac tcacaattcg gttcaccagg
    14521 cggcccacgc cttctacttc caccactact tcatcgccag gcaccacgtc agataagcct
    14581 tttggtgtgc cggtggcgat catgtcgccc ggatttaggg tcataaattc gcttaagtag
    14641 gcgatcagga agggcacgct gaagatcaga tcggcggtgg tgccttgctg gcgtaactcg
    14701 ccgttgacga aggtgcgaag ggtcagatta tgcgggtccg ggatcgcctc tttcggcacg
    14761 atggttgaaa gcatcggcgt cagtccgtcg cggcttttga cccgcaggtt agggcggtag
    14821 tagttttcca gatagtcgcg aatggcgtag tcgttacaca cggtgtagcc cgcgacataa
    14881 tccatggcat cggcttcgct gacgttacgc gcctgcttgc caataactac caccagctcc
    14941 gcttcgtagt gcatgtattc aatattgttc gggcgcacgg aggtctggtt gtcgccagtg
    15001 agggtattcg gcgctttcag gaacaccagc ggctcttccg gtggcttaaa ttccagttcg
    15061 ctggcgtggt cggcgtagtt caggccgagg gcaaacagcg taccgtgcgg gtgtggcagc
    15121 gttgggaagc tcttgcgcgt ggtcacttca cgttcgtcca ctaccggatt ttccagcggc
    15181 gggaaacctt ctgcgagaac acgcacgcga tcgcctggct gtatttccac gcgcgcctgt
    15241 ggcgtgccga gcagaatagc atcgcctgga ttcagcgtgg caaattcgct cagggcgctc
    15301 agcagctgtg cggcgttacg ttgtaaatcg gcggtattcc agtgatcggc aggacgcccg
    15361 ttgatctcgg tatagatagt cagattatcg acattgctga gagccacggt ttcgccaatg
    15421 gggcagaatc catcacgaca ttttgctttg attgccggac ggtaaaagct ctcttccggc
    15481 aggctgacgt cgttagccag cgcatatccg gcgatgtact ctgccgcatc ttcttcacgt
    15541 actttcgtcg ccgttttccc cacaatcaga gcaacagtcg caccgctcag tactttttca
    15601 ccctgtggaa agggaatcgg ttcaccgcaa ccaatcaccg tattgcgcgg tttaataaac
    15661 cagaccgcag ttttaggcgg ggctttgtag ggagattgct ggaacgcttc ctgccaggca
    15721 tcaagttggc tgcgatggtt caacgctacg gcgaagatag tgcctttcat taccactcct
    15781 cggattcgat catcttaatc attaatatgt taatgattac ttttattccc gattgcgtag
    15841 tttggcaaaa gtaagtgata taaatgtgat ctaattaaca aactatttac atttaaagat
    15901 aataaaatat taaatatcag taagttaata aaatatcatc aattttgata aaatgaagaa
    15961 ttgcgggggc agctcagtcc cggtaacgtg atgattaatt gtttctatat taatgatttt
    16021 gaatttttca ggggaaagat aatgcacgac tcactaacca ttgcgttgct ccaggcgcga
    16081 gaagcggcga tgagttattt tcgcccaatc gttaaacggc ataatttgac tgagcaacag
    16141 tggcgcatcg tgcgtattct ggcggaaagc acatcaatgg attttcacga tctggcgtat
    16201 cgcgcctgca ttttgcgccc aagcctgacc gggatcctca cgcggatgga acgcgacggt
    16261 ttagtgttgc gattaaagcc gatcaatgat cagcggaagc tctatatctc gctgaccaaa
    16321 gaggggcagg cgctgtataa ccgcgctcag acacagattg aagaagccta tcggcagatt
    16381 gaagctcagt ttactgccga gaaaatgcaa cagttaaccc atctgttaga ggaatttatt
    16441 gctctgggca attcccgaca agaagacatc ccgggcgata atgaataact ttttagtatc
    16501 tgataaccag cgcctggttt ttccatggat ggcgggttac accttttcat aaagtttttg
    16561 ctttccaggc cgaaaatctt gcatcggtcc acaggaaaga gaaaccatgt taaaacgtat
    16621 caagatcgtg accagcttac tgctggtttt ggccgttttt ggccttttac aactgacatc
    16681 aggcggtctg ttttttaatg ccttaaagaa tgacaaagaa aatttcactg ttttacaaac
    16741 cattcgccag cagcaatcca cgctgaatgg cagctgggtc gcgttgttgc agacgcgtaa
    16801 caccctcaac cgcgcgggta tccgctacat gatggatcag aataatattg gtagcggttc
    16861 aaccgttgct gagctgatgc agagtgccag tatttcgctg aaacaggcgg aaaaaaactg
    16921 ggcggattac gaagcgttgc cgcgtgaccc gtgtcagagc accgccgcag cggcagagat
    16981 caaacgtaat tacgatattt atcacaatgc gctggcggag ctgatccaac tgttaggtgc
    17041 aggcaaaatc aacgagttct ttgatcagcc gacccaggga tatcaggacg gtttcgagaa
    17101 gcagtatgtg gcttacatgg agcaaaacga tcggctctat gatatcgccg tcagcgataa
    17161 caatgcctcc tacagccagg cgatgtggat tctggtgggc gtgatgatcg tcgtactggc
    17221 ggtcatcttc gccgtctggt tcggtattaa agcctcgctg gtagcgccaa tgaatcgcct
    17281 gattgacagc attcgtcata ttgcaggcgg cgatctggtg aaaccgattg aggtggatgg
    17341 ctctaatgag atggggcaac tggcagagag tttgcgccat atgcagggag agctgatgcg
    17401 taccgtcggt gatgtgcgca acggggccaa tgccatctat agcggtgcca gcgaaatcgc
    17461 taccggcaat aatgatctct cttcgcgcac cgagcaacag gccgcttcgc tggaagagac
    17521 ggcagccagc atggagcaac tgaccgcaac ggtgaaacag aacgccgaga atgcgcgcca
    17581 ggccagccat ctggcattaa gtgcttctga aacggcgcaa cgcggcggca aagtggtaga
    17641 taacgtggtg cagactatgc gcgatatctc caccagttcg cagaaaatcg ccgatattat
    17701 cagcgtaatt gacggcatta ccttccagac caatattctg gctttgaacg cggcggttga
    17761 agcagcgcgt gcgggtgagc aaggacgtgg ttttgcggtg gttgcgggag aagtgcgtaa
    17821 tctggcccag cgtagcgccc aggcggctcg tgaaattaaa agcctgattg aagactcggt
    17881 ggggaaagtg gatgttggct ctacgctggt cgaaagcgcc ggggaaacaa tggcggagat
    17941 tgtcagtgct gtgacccgcg tgacggacat tatgggcgaa atagcttctg cttctgatga
    18001 gcagagccgt ggtatcgatc aggttggctt agcggttgct gagatggacc gggtaactca
    18061 acagaacgcc gcgctggtgg aagaatctgc cgctgccgcc gccgcgctgg aagagcaggc
    18121 cagtcgcctg accgaagctg tggcagtgtt ccggattcag caacagcagc aacagcagcg
    18181 tgaaacatcg gctgtggtaa aaaccgtgac gccagctacg ccgcgtaaaa tggcagtggc
    18241 agatagcggg gagaactggg aaacgtttta atcgccataa aaatgcccga taagcaaaat
    18301 gttatcgggc atagggagtt taatctttac gcgggtcgtt gatcggctgg cgaaccagga
    18361 agatgtacgc cagtgctccc gctgcggtga cgcaaccgca aatgattagt gccagacgga
    18421 atgagtgggt tgtatcaaca ataaaaccag taatgatcgg cgcgaaagag gcgcagatga
    18481 agctggcaaa gttctggata ctgcccaccg acgcagtcat gcgagaagca actgcgacgt
    18541 ggatcaagcc ccagcaggat gttccggcaa agtgaataca gaacagtgcc atgccaatca
    18601 gcagaaccgc catcatggat gttgtcgctt gtggtactat cagcgtaaag gcggcagaac
    18661 agaacatccc ggtaataatg cagatcttac ggcttttaat cggagccatt ccccctttga
    18721 ccagccagtc agtaacgtaa ccgttgacca gcatcccggc agccccaaac aggaaaggga
    18781 tagccgccat caaccctgtg ctttttaaat ctaggttata ggctgtttgc aggtaaccag
    18841 gaagccaggc cagatacagc cacgcagtgt agttgatgcc actgaatccg agcatcattc
    18901 cccacattgt gcggttacgg aacaggctgc gccattcggc aaaattgagc ggatctcggc
    18961 gggcattgac gctacctgca ttgagataag cttgttcaac ggcagtcagt tctacgtgct
    19021 cgcggttgcg atagagcata taccagccga tggcgagaaa aatccccagt acaccaatgg
    19081 taataaacat cccgcgccag cccatcacca gcatcattgc cgccagaatc ggtgggctta
    19141 cagcaacgcc aatggtagaa gctgcgttga agaagcccat cgggcgtccg cgctctttga
    19201 tgttgaacca gtcgttaatg acttttacac cgcatgggtt catcggcgct tcaccaatcc
    19261 ccataccgat acgcaccaac acgaactgcg taaagctgtg caccatgcca gacatggcct
    19321 ggaacagtga ccagaagaac atccccagtc ccagcatcag gcgtgggcct ttacgatcca
    19381 atagtgggcc gcaaggaagt tgcgcaatgc cgtaagcgag tgaaaacacg gagagcaaag
    19441 cgccgatttc ggtggcactt aatcccaatt tttcacgaat cgttaaattt gctaccgaca
    19501 gcgaactgcg gtcgagataa ttgattaccg ccgcaaaaaa taataacaac atggcggtgg
    19561 tttgaatgcg tttaattcgg atactgcgtt gaactaatcc atctggtggc acgggaatat
    19621 ctgccgacga agatggagta aatgaagaac gcggatcgag ggtgatattt tctttttcca
    19681 cgccggactc ctgtatttat aattttaatt gtttacgtaa ttgtgcatta cgcaaggttg
    19741 acattaacgg caggttaaag ttttaggact ttactaccgc tcacaaaaac atttttagtc
    19801 gactgattgt gagcataggt aagcgggaat tttccggtat ttatcttttg ataaaaccgt
    19861 gcacccgcta atagtttgct gtttttaatc cgtacttaat gagcataacg cgtgttctca
    19921 ttaatggatc gaatcattga ttgtttagct gaattcaaat ggttacggag
//
LOCUS       DADLSA010000070        19838 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.070, whole genome shotgun sequence.
ACCESSION   DADLSA010000070 DADLSA010000000
VERSION     DADLSA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.070"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..441
                     /locus_tag="JN691_002432"
     CDS             <1..441
                     /locus_tag="JN691_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HAY7243853.1"
                     /translation="DHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNLTAADGL
                     AVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMAGPQRVC
                     ASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(549..2087)
                     /gene="emrY"
                     /locus_tag="JN691_002433"
     CDS             complement(549..2087)
                     /gene="emrY"
                     /locus_tag="JN691_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAY7243854.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAICYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2087..3250)
                     /gene="emrK"
                     /locus_tag="JN691_002434"
     CDS             complement(2087..3250)
                     /gene="emrK"
                     /locus_tag="JN691_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAY7243855.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            3666..4280
                     /gene="evgA"
                     /locus_tag="JN691_002435"
     CDS             3666..4280
                     /gene="evgA"
                     /locus_tag="JN691_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAY7243856.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            4285..7878
                     /gene="evgS"
                     /locus_tag="JN691_002436"
     CDS             4285..7878
                     /gene="evgS"
                     /locus_tag="JN691_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAY7243857.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNNYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPSVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFKIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(7934..9079)
                     /gene="yfdE"
                     /locus_tag="JN691_002437"
     CDS             complement(7934..9079)
                     /gene="yfdE"
                     /locus_tag="JN691_002437"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAY7243858.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(9153..10097)
                     /gene="yfdV"
                     /locus_tag="JN691_002438"
     CDS             complement(9153..10097)
                     /gene="yfdV"
                     /locus_tag="JN691_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAY7243859.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLEPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(10167..11861)
                     /gene="oxc"
                     /locus_tag="JN691_002439"
     CDS             complement(10167..11861)
                     /gene="oxc"
                     /locus_tag="JN691_002439"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAY7243860.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(11915..13165)
                     /gene="frc"
                     /locus_tag="JN691_002440"
     CDS             complement(11915..13165)
                     /gene="frc"
                     /locus_tag="JN691_002440"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAY7243861.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(13678..14301)
                     /locus_tag="JN691_002441"
     CDS             complement(13678..14301)
                     /locus_tag="JN691_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAY7243862.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            14598..14873
                     /locus_tag="JN691_002442"
     CDS             14598..14873
                     /locus_tag="JN691_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243863.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(14950..15192)
                     /locus_tag="JN691_002443"
     CDS             complement(14950..15192)
                     /locus_tag="JN691_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAY7243864.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            15545..16465
                     /gene="lpxP"
                     /locus_tag="JN691_002444"
     CDS             15545..16465
                     /gene="lpxP"
                     /locus_tag="JN691_002444"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAY7243865.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMLGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTIVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            16820..16891
                     /gene="ypdK"
                     /locus_tag="JN691_002445"
     CDS             16820..16891
                     /gene="ypdK"
                     /locus_tag="JN691_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAY7243866.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(16956..18194)
                     /gene="alaC"
                     /locus_tag="JN691_002446"
     CDS             complement(16956..18194)
                     /gene="alaC"
                     /locus_tag="JN691_002446"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAY7243867.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            18571..>19838
                     /locus_tag="JN691_002447"
     CDS             18571..>19838
                     /locus_tag="JN691_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY7243868.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQ"
BASE COUNT         5628 a         4591 c         4220 g         5399 t
ORIGIN      
        1 gatcatgttc actgcttttt tgccgaacca acgcactccc cttgtatgtt gttaggcgtc
       61 catacaggat tacacgatca gatttctgtt caggatattg gtatcgacaa ccttaccgca
      121 gcggatggcc ttgcagttgg tcgcgcatca ggctttgtcg ggcgggcaat ggagcgtctg
      181 ctggatggct tctataccct tagcgatcaa accatgtatg acatgcttgg ctggctggcg
      241 caggaagaag gtattcgtct tgaaccttca gcactggcgg gtatggccgg acctcagcgc
      301 gtgtgtgcat cagtaagtta ccaacagatg cacggtttca gcgcagaaca attacgtaat
      361 gccactcatc tggtgtgggc gacgggaggt ggaatggtgc cggaagaaga gatgaatcaa
      421 tatctggcaa aaggccgtta ataacgtttc aacgcagcat cgcaatcctt tccctaggtg
      481 agcgatgctg ccgatggcgc agacttaaga tccccggtct tacccgctat aaccccctcc
      541 ttttatcatc acccaacgcc tttcgctgta aacggcggtt tcgcaaacca aaccaaaacc
      601 gtaagtaaga taaaagcaat agccgccata cggaaaatct catttgcaga aatagaaagt
      661 gactgctggg ttatttcatt attaatttca ttaagaactc ctgaaagcga accatagtat
      721 ttatccataa tttgtgacga tgaattaaac acggggttaa attgatcgat ggttgctgtc
      781 aactgactat ggtgtaacga ttcgcgtcgt ccccacagcg tcattgtcaa cgacgtacca
      841 actgatcctg acaaggtacg aaaaaaatta ctcatactcg aggcattggc aaatttatta
      901 tctggcaagc ctgaaaacga aatcgttgtt aagggtaaaa agaaacaggc aacggcgaat
      961 ccctggaaaa actgtggcaa aatgatgcct gtaaaatcaa tcgttggcat aaatgtcaca
     1021 gaacgccagt aatagcaaat cgcatacatc aaaaaactga atgtcactaa cacccgcatg
     1081 tctattttat tgccataacg tcctatcaaa ggtgaaatca atagtggcat gatgccgatg
     1141 ggcgcataag caagcccggc ccatatcgca ttatacccca tcgtttcctg gagtaactgc
     1201 ggcataagga cgatcgctcc agagtaaaat aaatacgcgc aggtgatact cacaatacca
     1261 atggtgaaat tacgggactt aaacaaactg agatcaagaa tcgggttctc tgaggtcgac
     1321 tcccaaatga ctaaagagat cagagaaata actgatacta ctgttaatat tattattgta
     1381 ctcgagttga accaatccag atcgcgccct ttatcaagca taatttgcaa gccaccaaca
     1441 ccgagcacta acagggtcag tcctggtaga ttcattttta ccggtgaagt ttcagtttct
     1501 cttcctttaa gtaaggttaa gcataatgtc aggacgataa tccccatagg gacattgatt
     1561 aaaaatatcc aaccccagct aaagttatcg caaatatagc cgcccaatat cggcccacat
     1621 atcggagcga taatcacggt cattgaccat aatgccagag caaatgttct tttttctggt
     1681 ggataattcc ttaataataa actctgtgac agtggaatta acggccccgc cattaatccc
     1741 tgaacgactc taaaaaatat cagcacatca agattggtcg ataaactaca cattaaggaa
     1801 gacagcgaaa aaaaagtgac tgaaagtaaa aataacctta attcgcctat tctttgtgcc
     1861 agcctgccgg taacagggat cgcaatggca tttgctacgc caaacgaggt gataacccag
     1921 gtgccttcgt ctgttgatgc tcccagaaag ccagatattg tcggtattgc gacgttagaa
     1981 atagtggaat ccaacatttg cataaacgtc gctaatgaca atgcaatagt aacgcaccat
     2041 aacgtcccac cggttaatgg tgccggagtt gatttagtga ttgccattaa agttgtccat
     2101 tatgcgaaat aatgttgcta atttcttttt ctatcggact ggtatcgata actaaagcct
     2161 tactggtata agcaggcatg gaggtcacgg ttgaagccag atcaggcatc tcagcaatgt
     2221 cttcgtcctt cgtatcaata gttgcagtca tcgataaacc aatgcgcaag gggtgttcca
     2281 tgagttcttt tggatcaaga gaaacttcaa ccggtacacg ctgaacgatt ttgatccagt
     2341 tccctgtcgc attttgtgca ggtaataagg agaacgcatt gccggttccc atattgatcc
     2401 ctgtcacccg accatgaaac acaacatttt caccataaag atcgctgata atattgactg
     2461 attgaccaat ccgtacatcc gtgagttgtg tttctttaaa gttggcatta acccacattt
     2521 gacgtgccgg tacgacagcc attaacgatt gtccggaact cactgtttcg ccgacctgaa
     2581 cacttcgctg ggcaatatag ccagtaaccg gactccttat atccgtacgt ttaagcacca
     2641 accaggcttc tttggttgca tccgccgctt caactacttg tggctgacgg tttaatggtg
     2701 tgttcattac taaagcttta ttcgctttat aagcctggat agcggcattc agtgccgctt
     2761 tgctacttat taacgtatct ttggtatgct ccagcgtttc ttttgaaata accccctgct
     2821 tcgctaaagg cacacgacgg ttataatctt ctaaagattg ttgatactga atacgtgctg
     2881 aagcgacttc ggcactgtat tgtttatcct gtaagtacag tttattcgtt tgccgaacaa
     2941 tatttgccag attattttta gctttattga gtgcgatagt ggcatcagtt ttatccagtg
     3001 aaactaaaat gtcaccctgt cgaacgtagt tcgtatcttt atgattaact acagtgacac
     3061 tacctgagac ttgtgcagaa attggatctg cattccccgt gacataggcg tcatctgtac
     3121 taatcatgtc ttctaattcc attgaccaat aggcataggc acctgaaaac gcaataaata
     3181 aaactatcac caataaagaa aagtattttt ttctgttaga ctgtttttta tttgaattaa
     3241 tctgttccac tattatctct catttcgcat agatgaaatt tatgaattga atgaaaagag
     3301 tattcattct tcatatcaaa cacagtgcat gtgttgcaca ttatcccagg aacgttggag
     3361 gcctaattta aatgaggtgc aaaaaaaaca acagaggact aaaccgtggc ttttgcaata
     3421 caattcttac gcctgtagga ttagtgagaa gacttatagt gctaacttga aactataaat
     3481 catcggtata atccctgatt ttattgttga catttcattt atgccgacta tttatatggt
     3541 atacttgtcg aattatctta aaggaagctc agattttctt atttttattg agaaaatgag
     3601 atgacgcctt atgtctgtat tactacaggg agaagggaga tgcttcattg caaagggaat
     3661 aatctatgaa cgcaataatt attgatgacc atcctcttgc tatcgcagca attcgtaatt
     3721 tattgatcaa aaacgatatt gaaatcttag cggagttaac tgaaggcgga agcgccgttc
     3781 agcgggtgga aacacttaag cctgatatcg tcatcattga tgtcgatatc cccggagtta
     3841 acggtatcca ggtgttagaa acgctgagga agcgccaata tagcggaatt attattatcg
     3901 tctccgctaa aaatgaccat ttttacggga aacattgtgc tgatgctggc gctaatggtt
     3961 tcgtgagtaa aaaagaaggc atgaacaata tcattgcggc tattgaagct gcaaaaaatg
     4021 gctactgcta tttccccttc tctctcaacc ggtttgttgg aagtttaacg tccgaccagc
     4081 aaaaactcga ctccttatcg aaacaagaaa ttagtgtcat gcggtatatt cttgatggca
     4141 aggataataa tgacattgcg gaaaaaatgt tcatcagcaa caaaactgtc agcacgtata
     4201 aaagtcgcct gatggaaaaa ttagaatgta aatcactgat ggatctttac acattcgcgc
     4261 aacgtaacaa aatcggctaa tcacatgaag tttttaccct atttttttct tctctgttgt
     4321 ggtctttggt cgaccataag tttcgcagac gaagattaca tcgaatatcg tggcatcagt
     4381 agtaacaacc gtgtcacact tgatccagta cgtctgagca acaaggaatt acgttggtta
     4441 gcgagcaaaa aaaatcttgt gattgcagta cacaagtccc aaacggctac cttgttgcat
     4501 accgattcgc agcaacaggt tcgtggtatt aatgctgatt atttaaatct tttaaaaaga
     4561 gcgttaaata tcaaattaac actccgggaa tacgcagatc atcaaaaagc gatggacgcg
     4621 cttgcagaag gtgaagtcga tatagtgtta tcacatttag ttacttcgcc gcctcttaat
     4681 aatgacattg ctgcaaccaa accattgata attacctttc cggcgctggt aaccaccctt
     4741 cacgactcaa tgcgaccgct tacctcacca aaaccagtaa atattgctcg ggtagcaaat
     4801 taccccccag acgaggtaat tcatcaatca tttccaaaag caacaattat ctcttttaca
     4861 aatttatatc aggcattagc atccgtctca gctgggcaca ataattactt tattggtagt
     4921 aacatcatta ccagcagtat gatttcccgc tatttcactc actccttaaa tgtagtgaaa
     4981 tattataact cgccgcgtca atataatttt ttcttgacca gaaaagaatc tgtcattctt
     5041 aatgaagtac tcaatagatt tgttgatgct ttaacaaatg aagttcgcta tgaagtatca
     5101 caaaattggc ttgatacagg aaacctggcc tttctgaaca aaccattaga actcactgaa
     5161 catgaaaaac agtggattaa gcagcatccc aatttaaagg tgctggaaaa tccttactcg
     5221 cccccctatt ctatgacgga tgaaaatggc tcggttcggg gcgttatggg ggacattctt
     5281 aatattatta ccttgcaaac aggtttaaat ttttctccga tcaccgtttc acacaatatc
     5341 catgctggaa cacagcttag ccccggagga tgggatataa tacctggcgc tatttatagt
     5401 gaagatcgag aaaataatgt tttatttgct gaagccttca taacaacgcc ttacgttttt
     5461 gtcatgcaaa aagcgcctga cagtgaacaa acattaaaaa aaggaatgaa agttgccatt
     5521 ccatattatt atgagctgca ttcgcaatta aaagagatgt atccggaggt tgaatggata
     5581 caggtcgata atgccagcgc tgcatttcac aaggttaagg aaggtgaact tgatgctctg
     5641 gtcgcgacac agctaaattc gcgttacatg atcgatcatt actatcctaa tgaactttat
     5701 cattttctta ttcctagcgt tccgaatgca tcgctttcgt tcgcttttcc tcgcggagaa
     5761 ccggaactta aggatattat taataaagca ctgaatgcaa ttcccccaag cgaagttctg
     5821 cgcctgacgg aaaaatggat taaaatgccc aatgtgacca ttgacacatg ggacctatat
     5881 agcgagcaat tttatattgt tacgacatta tccgttttat tagttggcag tagcctttta
     5941 tggggattct acctgttacg ctcagttcgt cgtcgtaaag tcattcaggg tgatttagaa
     6001 aaccaaatat cattccgaaa agcactctcg gattccttac cgaatccaac ttatgttgta
     6061 aactggcaag gtaatgtcat tagtcataat agtgcttttg aacattattt cactgcggat
     6121 tactacaaaa atgcaatgtt accattagaa aacagtgact caccctttaa agatgttttt
     6181 tctaatgcgc atgaagtcac agcagaaacg aaagaaaatc gaacaatata cacacaggta
     6241 tttaaaattg ataatggcat cgagaaaaga tgcattaatc actggcatac attatgcaat
     6301 cttcctgcaa gtgacaatgc agtatatatt tgtggttggc aagatattac tgaaacgcgt
     6361 gatctaatta atgcactcga ggtagaaaaa aataaagcga taaaggctac cgtagcaaaa
     6421 agtcagtttc tggcaacgat gagtcacgaa ataagaacac caataagctc tattatgggc
     6481 ttcctggaac ttctgtcggg ttctggtctt agcaaggagc aacgggtgga ggcgatttca
     6541 cttgcctacg ccaccggaca atcactcctc ggcttaattg gtgaaatcct tgatgtcgac
     6601 aaaattgaat cgggtaacta tcaacttcaa ccacaatggg tcgatatccc tactttagtc
     6661 cagaacactt gtcactcttt cggtgcgatt gctgcaagca aatcgatcgc attaagttgc
     6721 agcagtacat ttcctgatca ttacctggtt aagatcgacc ctcaggcgtt taagcaggtc
     6781 ttatcaaatt tactgagtaa tgctctcaaa tttaccaccg agggggcagt aaaaattacg
     6841 acctccctgg gtcacattga tgacaaccac gctgttatca aaatgacgat tatggattct
     6901 ggaagtggat tatcgcagga agaacaacaa caactgttta aacgctacag ccaaacaagt
     6961 gcaggtcgtc agcaaacagg ttctggttta ggcttaatga tctgcaaaga attaattaaa
     7021 aatatgcagg gcgatttatc attagaaagt catccaggca taggaacaac atttacgatc
     7081 acaatcccgg tagaaattag ccagcaagtg gcgactgtcg aggcaaaagc agaacaaccc
     7141 atcacactac ctgaaaagtt gagcatatta atcgcggatg atcatccgac caacaggcta
     7201 ttactcaaac gccagctaaa tctattagga tatgatgttg atgaagccac tgatggtgtg
     7261 caagcgctac acaaagtcag tatgcaacat tatgatctgc ttattactga cgttaatatg
     7321 ccgaatatgg atggttttga gttgactcgc aaactccgtg agcaaaattc ttccttaccc
     7381 atctgggggc ttacagccaa cgcacaggct aacgaacgtg aaaaagggtt aagttgcggc
     7441 atgaacttat gtttgttcaa accgttgacc ctggatgtac tgaaaacaca tttaagtcag
     7501 ttacaccagg ttgcgcatat tgcacctcag tatcgccacc ttgatatcga agccctgaaa
     7561 aataatacgg cgaatgatct acaactgatg caggagattc tcatgacttt ccagcatgaa
     7621 acgcataaag atctacccgc tgcgtttcat gcactagaag ctggcgataa cagaactttc
     7681 catcagtgta ttcatcgcat ccacggtgcg gctaacatcc tgaatttgca aaagttgatt
     7741 aatattagcc atcagttaga aataacacct gcttcagatg acagtaagcc tgaaattctt
     7801 cagctgctga actctgttaa agaacacatt gcagagctgg accaggagat tgctgttttc
     7861 tgtcagaaaa atgactaaat tgcggctccc acaatgttct aatgtgggag ctatttacca
     7921 gcacatctta catttatgat gagaactcct ggcgaatttg ttccccatgc tggtcgagcg
     7981 ttgccgctcc cggcataaca tgcgggtccg cgcagccgct gatttttatc ggattacccg
     8041 gcatcattat tcctccggct tcaatcaaca tatttctcgc ctgagtttgt ggcaaattaa
     8101 tggcctcagc cacacttaat aacggcgcga cgggcacacc aacttcatgt attttggcta
     8161 accaaacttc agctgcctgc gtttttaacg ttcgctcaat atattgttta agaatagcct
     8221 ggttttgtac gcgtaaaata ttgctgctaa atcggggatc attaaccagt tccgtaagct
     8281 ccagtgcctg gcataacgca gaaaaaagct tgtcattacc acaacaaatc gtaatcggct
     8341 tatcctgagt atcgaaaaca tcaaaaggtg ccatgtaggg atggcgattt cccaggcgtt
     8401 gtggtgactt ccctgtcgca atatatgcca tcagaccatg ctccagaaaa ctcagcgtgg
     8461 catcaaacat cgctatatcg acatgcgccc ctctctggct cttttcgcgg ccataaagtg
     8521 cactcactat tccgctgaat aaatagacac cgccgcatag atccgcaaga gaggtaccaa
     8581 cacgcactgg cggagcatca gggtaccctg tttccatcat tatcccactc attgcctgaa
     8641 tgatggtatc gtaggcagga gcatctttta gcggaccggt atgtccgaaa cctgacgatg
     8701 aagcatatat gaggcgcggg ttgatttctt gtaacctttc ccatgaaaac cccagttttt
     8761 ccattgtacc tgggcgaaaa ttctcagcta atacatcagc ttgtttgagc atatttataa
     8821 atatactttt atcatgatca ttctttaaat caagaaccac actctctttg ccatgattaa
     8881 taaaactgta atagagtgac tgtccatcca catagggacc aaatgtgcgg gtatcatcac
     8941 catgacctgg tggctcaact ttaattaccc ttgcgcccat attataaaga agttgagttc
     9001 cgaaaggtcc attaaggaca tgtgtcatat cgataactaa taagccttca aacggccctt
     9061 tgctttcatt atttgtcatt tgcttatgcc ttgaaaaaag ccgaaggtga aaagacactt
     9121 cggcatttat gtaaaatcaa taacatgatt aattatgaaa ccagtcgact gacataaatc
     9181 cacaagggag ccgtgacgac aaagcccagt acgctcacag ccaatgacgc tgtaccagta
     9241 cgggtgtaga cattaaaccg gctggcaata atgattccgg agaatgccgg cggtaatgcg
     9301 cctgccagta ccatcatctg caaatgttcg ctgttcaaat gacatgccat accaacgaga
     9361 agcagcgcca gtggcatcag aatcaacttc aggaaggtgt tataagcaat ttcggcactg
     9421 aactcgaatt tatgtgcagc cagagtcaac cccgcagcga ataccgctac ccctgagtta
     9481 gccttcgcaa tcagattaaa ggttgggtcc catgctgccg gaatttttac cccaaccagc
     9541 accaggatcg ttgccagaac aggtgcccat actactggct cctttgccgc agaaattaat
     9601 gcgctcagat tactattctt cttaccatcc gctcctgagg aaggattcag caaatacaga
     9661 ccaataggaa tagtaattgc gttaacaata atagaaataa ttgctaccac taaacctgtt
     9721 gatacggaat caccataaat aggctcgaga actgcaaacc ccaagaatcc aatggtaggt
     9781 gaacccgcaa ttaatgcaca tacagctgct tctgcatggg tacgtttaaa aaatttgtag
     9841 caaccaaacc aggagaaaaa gaaacatccg acaataacca caagtgatac aagggtcaga
     9901 cgagtgtccg caaaaatcat ttccctgttt gcccgggtaa tagaaacaaa tagagccgca
     9961 ggaagagcgt agtttaatac cagtttatta aatgcgcggg cttgatcttc tgaaaaagtt
    10021 tctcgtctgc cgctaaaata acccaacagc atgattacga taataggcaa taaatcgcca
    10081 ataaaaaatg ttagcataat agtatttccg ataagggtcc cctcaccatc gcataatgag
    10141 ttggcaagag gagacgatgt cagtatttaa ttaccagcga cttgttttgg gttaagtttg
    10201 gtaatatggc cactttcagt tcctgctgca gggtcgatga ccacattaat aatggtcggt
    10261 ttgcgcgact gaataccggt ggttaaagca tgacgaagtt catctgtcgt ggtgacgtta
    10321 tagccaacgc cacgaaacgc atccattaat ttgtcatacc ttgcatggtg caacagatcc
    10381 gttggtgatg gtgcaccagc gccactgaga tcaacaccgt ctcctctgta gatgccgcca
    10441 ttattaaaaa taacgatcgt caccggcagg ttatatcgac aaatcgtttc aatttccatc
    10501 ccactgaaac caaaagcact atcaccttca atggcgacaa ccggagaacc agaagtcacg
    10561 ctagcaccga tggcatagcc cataccgatg cccatgacac cccaggtacc acaatccaga
    10621 cgacgacgtg gtttatacat atcaataata tttcgtgcat tatccagggt atttgcacct
    10681 tcattaacta aataaatatc ctggttctcg cgcaatacgt cgcgcacagc acttaatgca
    10741 ttaaagtaat ttaatggttg agtatctgta cttaattttt catgcatttt ttgtgcattt
    10801 tgctgcttgt ggatatttaa aatatcgcgc cataccagtg gagtcgtaaa tgtgttttgt
    10861 ttcagttctg ccagcatacc ttgcatactg gatgcaatat caccaacgac tggcacagca
    10921 atggggcggt tgctgtcaat ttcctgcggt tcaatatcca gttgaataaa ctgtgtatct
    10981 gccgcccatc cttttttacc gtgtgccaat aaccaattca gtcgtgcacc aacaagcatg
    11041 acaacgtcag catttgccag ggcaaacgaa cgcgcagctg ccgcagaaag tggatgcgta
    11101 tcttcaagga tccctttcgc catagacatt ggcaggaatg gaatctgagc actttcaata
    11161 aattcacgaa gctgttcatc agcttgtgaa tacgccgcgc ctttgccaag gataattaat
    11221 ggccgttcag cttttgctaa aagcgaaatt gcgctagtga ctgacttcgg gcatggcaat
    11281 aatgctggcg acggattttc aactttaaca atcgtggtta acgcttcgtc tttttccatc
    11341 gtcgcggcca ggacatttgc tggcaaatca agataaactc cgccagggcg acccgataca
    11401 gagacccgga tagcgcgtgc caatgcaatg ccaagatcct gcggctgatt aacgcgaaat
    11461 gctgctttgg catacggttt tgccgcattc atttggtcca gctcttcata atcaccttgc
    11521 tgtaggtcga cgatcgcgcg gtcgctggag ccgctaatca tgatcatcgg aaaaccattt
    11581 accgttgcgt tggccaatgc ggtcaaacca ttgaggaatc ctggcgcaga aactgtcagg
    11641 cagatccccg gtttttgggt aagaaaaccg cttgccgcag cggcatagcc tgccgactgc
    11701 tcatgacgaa aaccaatata acgaatgcct tccgcctggg catggcgtgc catatccgtc
    11761 acaggaatac ctacaacacc ataaatagtg tcaatattat tctgttttaa tgcttcaacg
    11821 atgatatgca taccatctgt catttgaagt tgatctgaca tgattaataa cctcgaaatt
    11881 tttataaatt gggagccaga agcccccgtt aatatcagat ggcgtggttt tgcttcattg
    11941 cagcaatttc atcgtcgcta taacccagct cctgcaatac agcagcggta tgttcaccta
    12001 atagcggcgc agctttaata tccggcgtaa aggcagagaa tttcattgga caaccaacgg
    12061 tcagatattt tccacgcaac ggttgttcca cttcaacaac actgccactt tggcgcaaag
    12121 agggatcaag tgaaatttct ttcatactta aaaccggtgc acaaggaata tcaaactgag
    12181 tcaaataggc caccgcttca tgtttatcaa tagtgacagt gtatttttcg atttcagcaa
    12241 aaatatcgaa aatatgtggc tgtcgggcat gggctgtact gtatgccgga tcggtaatcc
    12301 attctggttt gccgatggct ttacaggtgt tttcccagtt ttgctcctga atagtgaaat
    12361 aaatataggc gttaggatcg gtttcccagc ctttacattt caggatccag ccaggctgac
    12421 cgccaccacc tgcattacca ccgcggggaa ctgcatcacc aaatgtacca ttcggatact
    12481 gcgggtattc ttccagataa cccaatttat cgagacgctg ctggtcacgt aatttcacgc
    12541 ggcaaaggtt caatacggca tcctgcattg acatggtgac tcgttgccca cgccccgttt
    12601 tttcgcgatg cagcaaagca gcaagtaaac cgatcagcaa atgcattccg gtgttgctgt
    12661 cacccaacgc tgcagcgctt accagcggcg gaccatccca aaaacccgta gtggatgccg
    12721 cgccacccgc tgcctgagca acgttttcat aggcttttac attcacataa ggcgaacact
    12781 catcaaaccc tttgatcgaa ccaaaaatca gacgtggatt gatttcttga atatgctccc
    12841 aggtgaagcc catgtgatca atggcccctg gatgaaagtt ctcgactaag atatcagctt
    12901 cgcggatcag cttttccatt acctctttgc cttccgctgt tttggtattt aactcaatag
    12961 aacgtttgtt actgttaagc atggtgaagt aaagcgcatc gatatcagga atatctcgca
    13021 gttggtgacg cgttacgtca ccaacgccgg gacgttcaat tttaataacg tcagcgccaa
    13081 accaggccag catttgagta caagatgggc cagattgcac accggtgaaa tcgagaactt
    13141 taattccttg aagtggagtt gacatagcaa tacctattag ttaattaaat atgtgttaat
    13201 ttagcgttta tttaaggcat aacgccctct ataaataatc tgcaaaatat cttcgtagcg
    13261 gcactcgata tttttaaatt cctgtatcaa gtactgaaaa ttatttcatc gccacttcca
    13321 attcctttct gcatggtaag tagtgaggac gaaaagaaga atatgataaa tattgattcg
    13381 tggtaatcag agagagtcgc gacatttgtg atgttattct tacaactgta agaagcgccg
    13441 gtgatttaca agtaagagta tgattacgct ttttaaagcg taccataaag cataacataa
    13501 taaatgattt gtgatgacac gggattattg cggtttactt tgaggaaaat aaaatgcgtg
    13561 tttatagaac cgcgagcgcc aaataatttt atgaccacaa cctgtcgtat tgtggtcata
    13621 aaaaattgtc ggctggaggg agttaaatcc tttacctcaa tatactgcct gtcagcatta
    13681 gttcacaaac agtgcttcgc tatcgacgat gattccatct tccgcacctt taagcgccag
    13741 gtttgcctcg tagtattgct ttttatcaag cagtttctgc gcatcagcaa gtgcattacg
    13801 agtctgttta agcggcatca gatattggtt ttccattacg ccaacaccgg ccaggcgaag
    13861 ctcttccatt gcgccctttt tatccccttt cgccattttt tcattggcaa tttttatcgc
    13921 ggcttctttt tcgggtgttg ctacataact ctcagaaatt ccgacagatg cattaatgac
    13981 aatatattgg tcatcattga gattggtctt tttacctggc ttagcgaact ttgcccattc
    14041 ggtactgtca tctgacagca aagcggaagc ttcattggtc agttcttttg ccttttccgg
    14101 atcgccatga aacagagcca ggcgcgctac ctgcacgtca cgcatcgcat ataaaccttg
    14161 ttcagagata cgctctgctg ctgccgtttt ctgggtttgc tgagtttgct gttgtgccgc
    14221 taccggtgca ttatcagcag cccatacagt tgaagatgcc agaattgctg tgaccatcgt
    14281 agccataatt aaacgtttca tacttacctc attctttaac agttttagaa aacgccgcca
    14341 tcatattggg tgagcatgaa atgagaaatc agagagcaac ggtcattttg aaagggttat
    14401 cttacaattg tagttattat cgcaaagcgg catttagtct tacagttgta ggaatatgct
    14461 tcctgtcgca gagaactatt cttatatacc acaaagagat gtcatttatt atattgatgt
    14521 aaatattgat taattatatg taaatacagc cataacatcg tatggttctc agtgaaccat
    14581 tttcaggata actttctatg aaagtaaact taatactttt cagcttattt ttattggtct
    14641 ctattatggc atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt gagaggagct
    14701 ataaagagac tgaaaaaaca tcagcgatga cgaccacaca ctctacaaaa cttcagccat
    14761 cacaggcgat tttgtttaag atgagagaag atgttccacc attaaacctc acagaagaaa
    14821 tcacgcctac ttatccgaca aaggcgaatt atcttattca tcctgtgcga tagcgtctgt
    14881 cacatggcag gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca aatgagccat
    14941 cctgacccac tatcgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg
    15001 ggcaagaaac ataaatatcc ccgtcaccag ttcattaccg ccagttaacc acatggtgaa
    15061 gtaataaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg ccgataccac
    15121 gttcagtacc tgaccgatgt agccgcttac gcagacaata cacagggcga gaaacatcca
    15181 taaataaatc atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta
    15241 atagtaagac aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcacgt
    15301 taaatgcaac gctattgcgt gcggaaaatt ttttactttg cacgattaat aatcagtagc
    15361 tgaaagcagt cagccagtga taaagtagag ccaccgcatt catgctcctc gtggttatgt
    15421 cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt
    15481 gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt
    15541 aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac
    15601 atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg ttctctgctt
    15661 tctcggcacg cgtattggcg caatggcgcg accattcctg aaacgtcgtg aatctatcgc
    15721 ccgtaaaaac ctggaacttt gtttcccgca gcattctgcg gaagaacgcg agaagatgat
    15781 tgccgaaaac tttcgttcac tcggcatggc gctggtagaa accggcatgg catggttctg
    15841 gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ttggataacc ttaaacgcgc
    15901 acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg
    15961 cggccgcgtg atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca
    16021 gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgctcggcag
    16081 aaataatctg cgcggcattg tcggtgcact gaagaaaggt gaagcggtat ggtttgctcc
    16141 cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt
    16201 cgccacaacc aatggcacct atgttctctc ccgtctctct ggcgcagcca tgttgaccgt
    16261 aacgatagta agaaaagcgg attacagcgg atatcgtttg ttcatcaccc cagagatgga
    16321 aggctacccg acagatgaaa atcaagccgc tgcctatatg aacaagatta tcgagaaaga
    16381 gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa cgcgcccggt
    16441 gggagaatcg tcgttgtaca tttaacaaac tgctcttact ctttccctcc tcaaatcggc
    16501 cataataacc tcatgatttt tatggctttt ttcttaagtt ttttccttcg ctgaatatct
    16561 aattattacc gtcagaaatt aaactgtcgc cgatatgaga cacttgtaag ttactaaaag
    16621 taaataagaa aatgcctctt gtctcgtttc agttttaggc gtacattagc gccgtctcga
    16681 gcagtttagc tcagaattct ggggtgatgc ccacggtaaa atacagggct tttaaggtcg
    16741 ctttgcccgg tgtcaactca ctgtatcagg tgtaatgaag tcattcaggc gtaacagtaa
    16801 ttacgcggag agatgtaaag tgaaatattt ctttatgggc atttctttta tggttatcgt
    16861 ttgggccggt acttttgccc tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc
    16921 catcaggctc ctgtttgcat ttctgacgac ggtgtttatt ccgcgttttc gtgaatatgt
    16981 ttgctgctgg cgggtagtaa accgtcggca cggaacatcg ctttaatccc tctgatggcc
    17041 tgacgaatac ggtcgcggtt ttcaatcagt gcaaagcgaa catgcgtatc accatagtcg
    17101 ccaaagccaa tccccggcga gacacaaacc ttcgcttcgt taaggagctt cttggcaaat
    17161 tccagcgatc ccatggccgc atatggttcc gggattttcg cccagacata catcgaagcc
    17221 ttcggcattt cgaccatcca gcccgcttca tgcagccctt taaccaatac atcacgacgg
    17281 cgtttgtact gttcggcaat gtcgcgcacg cactgttgat cgccctcgag tgccgcaatc
    17341 gccgccacct gcaacggcgt aaaagtgccg taatcgtgat agcttttgat tcgcgccagg
    17401 gcactgacca gcgttttgtt gccgaccata aagccgatac gccagcccgc catgttgtag
    17461 cttttcgaca gcgtaaagaa ctcgaccgcc acatcgcgtg cacccggcac ctgcataatt
    17521 gacggcgctt tccagccatc gtagacgata tcggcatagg ccaggtcatg gaccaccagc
    17581 acatcgtagc gtttcgccag cgccacaacc ttttcgaaga actccagttc cacgcattgc
    17641 gcggttgggt tcgacgggaa gccgaggatc atcatcttcg gtttcggata actttcacga
    17701 atggcgcgct ccagttcgtt gaagaaatcg acaccttcca ccagcggcac tgagcgtacc
    17761 tgcgccccgg caatcaccgc gccataaata tggatcgggt aacttggatt cggcaccagc
    17821 accgtgtcac catgatccag cgtcgccagc atcagatgcg ccaggccctc tttcgaacca
    17881 atagtgacga tggcttctga ttccgggtcg atttcaacgt cgtagcgatc ctgataccag
    17941 cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc atgcgtgtcc
    18001 gggcgctggg ccacagtaca taatttttcg acgatatgcg gcggagtcgc accgtccggg
    18061 ttacccatgc tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat tttcagttca
    18121 gcggtgatgt taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg
    18181 cgagtgtcag ccataatttc ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct
    18241 gccacaatgg tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc agcgttttag
    18301 aacattttta tcaatggctt taaatgagga actccacttc aaaaacagga aatttttcta
    18361 cgctaacaag aaaattacca ccaattttat taacattata atttcaataa atttaccagt
    18421 cagaagtgca ttatcaagag atacattgct aatacaccat ttatctttca atcccctttt
    18481 aaatttatgg tttttcctca aaaacggcct gctggtcatc agccttcagg ttacctatca
    18541 tagaggttta atccttattc agagtcaccc gtgcacgaaa tattcaacat gctgctggcg
    18601 gtcttcgatc gggcagcgtt aatgcttatc tgcctgttct ttctcatccg tatccgcctg
    18661 tttcgcgaac tgttgcacaa gtcggcgcac tccccaaaag aattgctcgc cgttaccgcc
    18721 attttctcgc tgttcgccct gttcagcacc tggtccggcg ttcccgtaga aggctcgctg
    18781 gtgaacgtac gtattatcgc ggtgatgtcc ggcgggattc tgtttggccc gtgggtaggc
    18841 atcattaccg gcgtgattgc gggtattcac cggtatttaa ttgatatcgg cggcgtgacg
    18901 gcgatcccct gctttatcac cagcattctg gcgggttgta tatcaggctg gataaacctg
    18961 aaaatcccca aagcacagcg ctggcgcgtc ggtattctcg gcggcatgtt gtgtgagacg
    19021 ttgaccatga ttctggtaat tgtctgggca ccaactaccg cgttggggat cgatatcgtc
    19081 tctaaaatcg gcattccaat gatcctcggt agcgtctgta tcggctttat tgtgcttctg
    19141 gtgcaaagcg ttgagggcga aaaagaggcc agcgccgcgc ggcaggccaa gctggcgctg
    19201 gatatcgcca acaaaacgct accgctgttt cgccatgtca atagcgagtc attacgcaag
    19261 gtctgcgaaa ttatccgcga tgacattcac gccgatgcgg tggcgattac taataccgat
    19321 catgtgctgg cctatgttgg cgtgggtgaa cataactatc agaatggcga tgacttcatt
    19381 agcccgacta cccgtcaggc gatgaattac ggaaaaatca tcattaaaaa caatgatgaa
    19441 gcccaccgca caccagagat tcattccatg ctggtgatcc cattgtggga gaaaggggtc
    19501 gtgaccggaa cgctgaaaat ttactactgc cacgcgcatc agatcacctc gtcattacag
    19561 gaaatggcgg tcggtctgtc gcaaatcatc tccacgcaac tggaggtttc acgcgccgag
    19621 cagctacgtg aaatggcaaa taaggcagag cttcgcgccc tgcaaagcaa aattaatccc
    19681 cattttctgt ttaacgctct gaacgctatt tcatcgtcaa tccgtctgaa tccggatacc
    19741 gctcgccagt tgatctttaa tctgtcgcgt tatctgcgct ataacattga attaaaagac
    19801 gatgagcaaa tcgatatcaa aaaagagctg tatcaaat
//
LOCUS       DADLSA010000071        19697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.071, whole genome shotgun sequence.
ACCESSION   DADLSA010000071 DADLSA010000000
VERSION     DADLSA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.071"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            206..1303
                     /gene="mltA"
                     /locus_tag="JN691_002448"
     CDS             206..1303
                     /gene="mltA"
                     /locus_tag="JN691_002448"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAY7243869.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1542..2348
                     /gene="tcdA"
                     /locus_tag="JN691_002449"
     CDS             1542..2348
                     /gene="tcdA"
                     /locus_tag="JN691_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAY7243870.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2399..2842)
                     /gene="csdE"
                     /locus_tag="JN691_002450"
     CDS             complement(2399..2842)
                     /gene="csdE"
                     /locus_tag="JN691_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAY7243871.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2842..4047)
                     /gene="csdA"
                     /locus_tag="JN691_002451"
     CDS             complement(2842..4047)
                     /gene="csdA"
                     /locus_tag="JN691_002451"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAY7243872.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4239..4466
                     /locus_tag="JN691_002452"
     CDS             4239..4466
                     /locus_tag="JN691_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAY7243873.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4817..5734
                     /locus_tag="JN691_002453"
     CDS             4817..5734
                     /locus_tag="JN691_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAY7243874.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5753..6148
                     /locus_tag="JN691_002454"
     CDS             5753..6148
                     /locus_tag="JN691_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAY7243875.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6141..7241
                     /gene="rlmM"
                     /locus_tag="JN691_002455"
     CDS             6141..7241
                     /gene="rlmM"
                     /locus_tag="JN691_002455"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAY7243876.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7285..8016)
                     /gene="fucR"
                     /locus_tag="JN691_002456"
     CDS             complement(7285..8016)
                     /gene="fucR"
                     /locus_tag="JN691_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAY7243877.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8074..8496)
                     /gene="fucU"
                     /locus_tag="JN691_002457"
     CDS             complement(8074..8496)
                     /gene="fucU"
                     /locus_tag="JN691_002457"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAY7243878.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8498..9916)
                     /gene="fucK"
                     /locus_tag="JN691_002458"
     CDS             complement(8498..9916)
                     /gene="fucK"
                     /locus_tag="JN691_002458"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HAY7243879.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVNKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAWLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     YELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10025..11800)
                     /gene="fucI"
                     /locus_tag="JN691_002459"
                     /pseudo
     CDS             complement(10025..11800)
                     /gene="fucI"
                     /locus_tag="JN691_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            complement(11833..13149)
                     /gene="fucP"
                     /locus_tag="JN691_002460"
     CDS             complement(11833..13149)
                     /gene="fucP"
                     /locus_tag="JN691_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAY7243880.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13696..14343
                     /gene="fucA"
                     /locus_tag="JN691_002461"
     CDS             13696..14343
                     /gene="fucA"
                     /locus_tag="JN691_002461"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAY7243881.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14371..15519
                     /gene="fucO"
                     /locus_tag="JN691_002462"
     CDS             14371..15519
                     /gene="fucO"
                     /locus_tag="JN691_002462"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAY7243882.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15572..16417)
                     /gene="xni"
                     /locus_tag="JN691_002463"
     CDS             complement(15572..16417)
                     /gene="xni"
                     /locus_tag="JN691_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAY7243883.1"
                     /translation="MRGLFLFSQPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKADRPP
                     MPEELHDEMPALRAAFEQRGVPCWSASGNEADDLAATLAVKVTQAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(16438..17805)
                     /gene="sdaB"
                     /locus_tag="JN691_002464"
     CDS             complement(16438..17805)
                     /gene="sdaB"
                     /locus_tag="JN691_002464"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAY7243884.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17863..19152)
                     /locus_tag="JN691_002465"
     CDS             complement(17863..19152)
                     /locus_tag="JN691_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY7243885.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4865 a         4826 c         5196 g         4810 t
ORIGIN      
        1 gtggatgcgc actatactcc gctcgcgttt tcatgcaacc ttttttttac cccgatcacg
       61 gatttagcat gattttgaac aaaaacaaac cactaaagcc tattttttgt gcaaaatttg
      121 cgtcagggag aggctgctca tttcattaaa cgcggcggtt tgttatcttc gttgcgcctt
      181 attttttaac ctgaagaaga gaacaatgaa aggacgttgg gtaaagtacc ttcttatggg
      241 cacggttgtg gcaatgcttg ccgcctgctc ttccaaacca accgatcgcg gacagcaata
      301 taaagacggg aaatttaccc agcctttctc tctggtgaac cagccagatg ccgttggcgc
      361 gccgattaac gccggtgatt ttgccgagca aattaaccat atccgtaatt cgtcaccgcg
      421 tctgtatggc aaccagagta atgtttataa cgcggtgcaa gagtggctgc gcgcaggcgg
      481 tgatacccgc aatatgcgcc agttcggcat tgatgcctgg cagatggaag gtgccgacaa
      541 ctatggtaac gtgcagttta caggctatta cacgccggta attcaggcgc gccatacccg
      601 ccagggcgag ttccagtatc ctatttaccg tatgccgcca aaacgtggtc gtctgccgtc
      661 tcgtgcggag atctacgcgg gggcattggg cgataaatac atcctcgctt acagcaactc
      721 tctgatggat aacttcatta tggatgtgca gggtagtggg tatatcgact ttggtgatgg
      781 cagtccgctt aactttttca gctatgcagg gaaaaacggt catgcctatc gcagcattgg
      841 taaggtgctg atcgaccgtg gcgaagtgaa aaaagaagat atgtcgatgc aggcgattcg
      901 tcattggggc gaaacacaca gtgaagccga ggttcgcgag ctgctggaac agaacccgtc
      961 tttcgtcttc tttaaaccgc aatcttttgc accggtgaaa ggggcaagtg cggtgccgct
     1021 ggttggccgt gcttctgttg cctccgatcg ttcaattatt ccgccaggta ctaccttgct
     1081 ggcagaagtg ccgctgctgg ataataacgg caaatttaac ggtcagtacg aattgcgtct
     1141 gatggtggcg ttggatgtcg ggggcgcaat caaaggccaa cacttcgata tctatcaagg
     1201 gatcgggccg gaagccggac accgcgcagg ttggtacaac cactatggac gggtttgggt
     1261 gctgaaaacg gctccgggcg caggtaacgt ctttagcggc tgatgtgata ttctgtatgc
     1321 cgtttttacg gatatgacag ggtgaggggt aacctcacat cctgattatt gactaagtgc
     1381 gcgttgttca taccggatgc ggcgtgaacg ccttatccgg cctacaaatc atgcaaatcc
     1441 aaaatattgc aggagttatg taggcctgat aagcgtagcg catcaggcat ttattcgttt
     1501 agggtgaggg ataacctcgc cctttttaat tctgaggtgc tatgtctgtg gtaattagtg
     1561 atgcatggcg tcagcgtttt ggtggcacag cgcgtctgta tggtgaaaaa gcgttgcaac
     1621 tgtttgctga cgcgcatatt tgtgtggttg gtatcggtgg tgtcggttcc tgggcggcgg
     1681 aagcgctggc gcgcacgggg attggcgcaa tcacgcttat cgatatggat gatgtgtgcg
     1741 tcaccaatac caatcggcaa attcacgctc tgcgcgataa cgtcgggctg gcaaaagcgg
     1801 aagtcatggc ggagcgcatt cgccagatta acccggagtg ccgtgtaacg gtggtggatg
     1861 atttcgtgac gccggataac gtagcgcaat atatgagtgc cggttattcg tacgtgattg
     1921 atgccattga tagtgtacgg cccaaagcgg cgctgattgc ttattgtcgg cgcaataaaa
     1981 tcccgctggt tacgaccggt ggcgcgggtg ggcagattga cccgacgcag attcaggtta
     2041 ccgatctggc gaaaacgatt caggatccgc tggcggcgaa gttgcgcgag cgcctgaaaa
     2101 gcgattttgg cgtagtgaaa aacagtaaag gtaagctcgg cgtggattgc gtgttttcta
     2161 ctgaagcgct ggtgtacccg cagtcagacg gtacggtgtg tgcgatgaaa gccacggcag
     2221 aagggccgaa gcggatggat tgtgcatctg gatttggcgc ggcaacgatg gtgaccgcca
     2281 cctttgggtt tgttgcggtt tctcatgcgc tgaagaagat gatggcgaaa gcggcgcgtc
     2341 agggttaaac aatcaggtcg gatgagacgc gaacgtctta tccgacccgg ttctgtgctt
     2401 aaacctgctt cgtcgcagcg ataatcgcct cgcttaacgc atttaacccc tggctgcgtg
     2461 aggcgctaag ctgcgcacgt aatcccagct catcaaacaa tgccagcggc gactgtgcct
     2521 gtaattcggc ggcggttttc ccctcaacgg cagtcaacaa caccgccaac aggccgcgca
     2581 caatgcgccc ttcgctgtcg ccaaagaagt gcattttgcc gttttcagcc acggtatatc
     2641 ccagccagac gcggttttcg catccggcaa tctctttagc ctgcgctttt aactcgtctg
     2701 gcaaagccgg aagctgtttc cccagcatga tcaactggcg atatttatct tcccattgcg
     2761 acaacggtgc gaaggtattg cgtaacgttt ctgcggttac ggttgtgccg aacggatgtc
     2821 cggcgaattg cgggtttgtc attaatccac caataattcc agcgcgcggt caacggcatt
     2881 caccagcgca tccacatcac tctttgtatt atatggcgca aatgaggcgc gcagtgtgcc
     2941 ggttacgcct aattctgcca gtagcggctg agcgcaatgt tgcccggccc gcaaggcaat
     3001 accgtactcc gccagtagcg tcaccatatc gctgtgatga acgccagcaa aatcaaaggc
     3061 cagcaggctg gaatcctggc agcggaatga acgaaagcct ggacgtttcg ccagcgcttc
     3121 ttctgcaagc gttgctaagc tacggctcca gctttcggcc tggttgatat cgtaatctgc
     3181 cagccattcc agcgccgcgc ttaatcctat gacgccagcg acatttggcg ttcctgcttc
     3241 cagtttccac ggcgcagatt gagtggtgaa accgtcaaaa ctcacttcgt gaatcatttt
     3301 gccgccaccc agccagggcg acatcgcttc cagcagttct gatttgccat acagcacgcc
     3361 gatacccgtc gggccataca gtttgtgacc agagaaagca tagaaatcga tatccagttg
     3421 ctgaacatcc gcggggaaat gcaccgcccc ctgagcacca tcaaccatca ccaccatccc
     3481 ggctgaatga gcaaaggtaa tcgctcgcgc cagatccggg caaccgccag tcacgttcga
     3541 catctgaccc aacgccagaa tccgactacg gggagtaatc agttctggca acaaatcgac
     3601 atccggcagt cgctgcgcat taagcggcaa tttcaccact ttggctctag tttgttgggc
     3661 gaccatcagc caggggacga ggttagcgtg gtgttctgcc acgctgacaa taatctcatc
     3721 gcccggttgc agacgcggac gcgcatagca ttgtgccacc atgttgatgg attcagtggt
     3781 gccgcgcgtc cagacgatag ttttatcatc cggtgcattc agtaattgcg ccactttctc
     3841 tcgtgcagct tcataacgcg cggtcaggcg ttgggcttcg gcaaactggc tgcgatggac
     3901 gtttccggcg ctcaggctgt aaaactgtcg agtggcttca accacggctt caggtttaag
     3961 cgcggtcgcg gcgctgtcga gatagacgcc cgcatcctgt agtgcgggaa actgggcgcg
     4021 aaactgcgcg ggattaaaaa cgttcatggt actcctcggc ttgatgcacc gatcgtgacg
     4081 caaaattcac tttgatacaa ggagagtgct aaccatcaga attatctgga tatcctggcg
     4141 aagccgcgta agtaacatat gctgaatagt gttaggcgtt aattttagcc tgttaatcaa
     4201 tactattaag atttattaat cgctttaagg agaagatgat gaaaaagact gccgcaatta
     4261 tttctgcctg tatgctgact tttgccctga gcgcctgttc cggttcgaac tatgtgatgc
     4321 acaccaatga cggacgtacc atcgtctctg acggcaaacc acagactgat aacgataccg
     4381 gtatgatttc gtataaagac gctaatggca acaaacagca gatcaaccgt actgacgtga
     4441 aagagatggt cgaactggaa cagtaattcg cgatcgcaag gtaaaaaaaa gcaccgcaat
     4501 taggcggtgc tacattaatc actatggaca gacagggtaa atgtacagga agtgaaaaaa
     4561 ggtagctttg ctaccatggt ctgaatcgca gaccaattgc aaacgcaaca acacaacatc
     4621 acaaccgtaa gccaaaagtt caccagaata cgcattccga taaaactttt cgttccggct
     4681 caggaagtgc cgccactata ggtatttgct ggtagaagct caacggacaa tttataatgg
     4741 ctcagattaa aaaaactaat aggttacaca gtgtgatcta attgttaaat tcatttaaca
     4801 tcaaagttta aaagccatgt ctaaacgatt accaccgcta aatgccttac gagtttttga
     4861 tgccgcagca cgccatttaa gtttcactcg cgcagcagaa gagctttttg tgacccaggc
     4921 cgcagtaagt catcaaatca agtctcttga ggattttttg gggctaaaac tgttccgccg
     4981 ccgtaatcgt tcactcctgc tgaccgagga agggcaaagc tatttcctcg atatcaaaga
     5041 gatattttcg caattaaccg aagcgacgcg taaactccag gcccgtagcg ccaagggggc
     5101 gttgacggtc agtttactcc ccagtttcgc cattcattgg ttggttccgc gactttccag
     5161 ctttaattca gcttatccgg gaattgacgt tcgaatccag gcggttgatc gtcaggaaga
     5221 taagctggcg gatgatgttg atgtggcgat attttatggt cggggcaact ggccggggct
     5281 acgggtggaa aaactgtacg ccgaatattt attgccggtg tgttcgccgc tactgctgac
     5341 aggcgaaaaa cccttgaaga ccccggaaga tctggctaaa catacgttat tacatgatgc
     5401 gtcacgccgt gactggcaga catatacccg acagttgggg ttaaatcata tcaacgttca
     5461 gcaagggcca atttttagtc atagcgccat ggtgctgcaa gcggctattc acgggcaggg
     5521 agtggcgctg gcaaataacg tgatggcgca atctgaaatc gaggccggac gtcttgtttg
     5581 cccgtttaat gatgttctgg tcagtaaaaa tgctttttat ctggtttgtc atgacagcca
     5641 ggcagaactg ggtaaaatag ccgcctttcg ccaatggatc ctggcgaaag ccgctgctga
     5701 acaagaaaaa ttccgctttc gttatgaaca ataatttacg tagggtacga ccatgaccag
     5761 ccgttttatg ctgattttcg ccgccattag cggcttcatt tttgtggcac tgggcgcttt
     5821 tggtgcgcat gtgttaagta aaaccatggg ggccgttgag atgggctgga tccaaaccgg
     5881 tctcgaatac caggcgtttc atacgctggc gatcttaggt ctggcggtgg caatgcagcg
     5941 tcgcatcagt atctggtttt actggagtag cgttttcctc gcgttaggca cggtgctgtt
     6001 cagcggcagc ctttattgcc tggcgctgtc ccatctgcgt ttgtgggcgt ttgtcacacc
     6061 ggttggcggc gtgagcttcc ttgcgggctg ggcgttaatg ttagttggtg ctatccgttt
     6121 aaagcgcaag ggcgtaagtc atgaataagg ttgtattgct gtgccgtccg ggctttgaaa
     6181 aagagtgcgc cgcagaaatt accgataaag ccggccagcg ggaaattttc ggttttgccc
     6241 gcgtgaaaga gaatgcgggt tatgtcattt atgaatgtta tcaacctgat gatggcgata
     6301 agttaatccg tgagctgccg ttcagttcat taatttttgc ccgccagtgg tttgtggtgg
     6361 gggaactcct gcagcatttg ccgccagaag atcgtattac ccccattgtc ggcatgttac
     6421 agggtgtagt agagaagggc ggtgaactgc gtgttgaagt tgccgatacc aacgaaagca
     6481 aagagttgct gaaattctgc cgtaagttca ctgttccgct acgcgctgcc ttgcgcgatg
     6541 ctggggtgct ggcgaactat gaaacgccga agcgtccggt tgtgcatgta ttctttattg
     6601 caccaggatg ctgctatacc ggttactcat acagcaacaa taattcgccg ttctatatgg
     6661 gcattccgcg cctgaaattt ccggcagagg cgccgagtcg ttccacgctc aaactggaag
     6721 aggcatttca tgtgtttatt cctgcggatg agtgggatga acgcctggcg aacgggatgt
     6781 gggcggtgga tttaggcgct tgccctggcg gctggaccta ccaactggtg aagcgcaaca
     6841 tgtgggttta ttccgtcgac aacggcccga tggcgcaaag tctgatggat accgggcagg
     6901 tgacgtggct gcgggaagac ggtttcaaat tccgtccgac acgcagcaat atctcctgga
     6961 tggtatgcga tatggttgaa aaaccggcta aagttgcggc attgatggcg cagtggctgg
     7021 ttaatggctg gtgccgtgaa accattttca atctcaaact gccgatgaaa aaacgctacg
     7081 aagaagtatc acacaatctg gcgtatattc aggcacagct tgatgaacat ggcataaatg
     7141 ctcagattca ggcgcggcag ttgtatcacg atcgcgaaga agtgacggtg cacgtccgcc
     7201 gcatctgggc tgcggtgggt ggtcgtcgcg acgagcgata acaggcaagg ccggggcgtc
     7261 gtctccggcc tgctaccctt tccgtcaggc tgttaccaaa gaagttgcaa cctggcgctc
     7321 atcagaaata atgtgtgtta cctcatccag atgcccgatg cgggcttccc ctgaacgatt
     7381 aaatttactt ttatcaatca ataacaacga ttgcgcggca cgttttaata gcatcgattt
     7441 gtaatcagcg ttgatcgcat tggagtccca cagtgcgccg ctgctatcga tcccttcaca
     7501 agaaaaaata aacagatcga tttccagcga tttaagttgg gaaatcagcg agggattgac
     7561 gtaacagcca tatttgcgct caagtgtgcc gccggaactg atcagttgaa tgcgttcgcg
     7621 tttaccgagt tcatggcaaa tcggatggct attggtgaag acctgaatgt tgatgtcagg
     7681 caactggcgt gccagatacc agcaagttga actggcgtct aaggctatca ccatcccttc
     7741 ttcaatccac gcgagcgcct cgcgcgcgat atctgcttta tgcgcataat ggcttttcag
     7801 cctgatgtga aagggatcgc cactgtcttg attttgacgg tggatatatt tagcgcgtcc
     7861 atgattgcgc agaattttac cctgcgtctg taattcattg agatcgcgac gaatggtttc
     7921 tttactgacc tttagctgtt cagagagagc ttccgtggtc aggctggtat ggttcagcag
     7981 caggtcgact atcgcttgct ggcgtgccgc tttcatattt ttcaccccat cattcgataa
     8041 ccgcccgcgt catgcgggcg taccggtatg agattacggt gttacccctt tttttaaaag
     8101 aatattcccg tacttcgctc gttcgcctgt gataacgatc gcaaaggctt tttgcgcccg
     8161 ttcataaaac gcaaaacgat tgatgcggat gatgtcagga cacggggctt gtagtgaaag
     8221 cgcattacgg taacgtcgtt ctacttcagg atcgagagtg tcaccctcta ccgccgccat
     8281 catcaccagc ggcggggcat aactgtccag ctcaaataac gggataatcg cctggagcaa
     8341 gtcgctcacc aacaggccat cagcgcggat cacctgcggt cccatcgaat gggcgggaaa
     8401 gtgagcatcg gaaaaaataa tttcatctcc atgtcccatc tctgccagca ctttcaatag
     8461 ttcgggagaa attaacggcg aaattgtttt cagcatttca cacttcctct ataaattcag
     8521 gttcagtttg cgggtagaaa taacggtact gataatgaat ctgtgcacgg gcttcttccg
     8581 ggctgttaaa ttcccctacg ccataccaac cgaacagcgc agctcctgcg acggtcgttt
     8641 cggcatcgtc gagaactttt accggaatat caagcatatt ggctttaatc tgattccaca
     8701 atgtgttgcg acttcctcca ccgactaaca ataattcata ggctttaaag tgcccgattt
     8761 tttccagcat ctgtagattg cgctgtaatt gcgcagttaa cccttccagc gccgcgcgat
     8821 agaaatgccc ccgcgtggta ttaagcgtca ctccttgcca gccagcgttc tgacacgaca
     8881 ataaatcaca ctgcattttc acaccatccg cgccaggtgc gatcagacga gcttcttcaa
     8941 tcaacatttg ccagggtgtt tcagccgtcc agaacagttt tctcacccat tccagcacgc
     9001 cggatgccag ccattgcata cctgggttat acaaccatgc ctggctatct agttcgcagg
     9061 tggaaccggc gtactgactt aacagcgaag tatcaacctg ggcgctgcga accattaaaa
     9121 tttcccatgt accggaggag agcacgggtt cattttgttc ggcaccagcg ccaaaaaggg
     9181 cgaactgggt atcgtgacct gcggaaatca ccggtatgcc aacgggtaag ccgagcattg
     9241 ctgcggcgct gttctgtagc gtaccaatct gttcacccgc ttccaccaga cgagggaaga
     9301 gtcggcgtgg aataccggtg gcttgtaaaa tttgcggact gaaatcgcgt tgctggatat
     9361 ccagcatctg gctggttccg gccatcgtga tatcagtagt gaattcgccg gttaaacggt
     9421 ggttaatcag cgacgaaatg aagagccagg cgtgcgcgcg ttccagcagt tgtggatgat
     9481 tttctttcaa ccacaccaac ttatataacg tattgaaact aaaggctccg acgccagaaa
     9541 tagcctgcaa ccgctgtgcg gagattaacc gttcaatatt gtccataacc gctgctgttc
     9601 gcggacattt ccagctaatg atcggataga gcagattgcc ttgcttattt accagagcgc
     9661 catccacacc aaaggtggtg acggcgatac cgcggatgtg gcattcagtc agttcactat
     9721 tgatttgccg acagcaatca gcaaagcgtt gcaaaatggc gtctaaagac cactggtgcc
     9781 aggtgttgtt ttccatcgcg atatcgctgg cattaggcgt tgaggcgcgg gcaacaattt
     9841 ttccctgccg attaaccgcg atggccctga cattggtcgc gccacagtcg agtaccagga
     9901 taacttcttg tttcataatc gctcctgcaa tatagccgga taacattgtt tatccggcta
     9961 accactcttg cttgttcagg cccggtagcc ggagcgaccg ggcatcacat cagggagtaa
    10021 tgtattaacg cttgtacaac ggaccgtagt tctggcaagc gcggtaatcc tggccttcaa
    10081 tatccatgcc gtgcgcagcc caggcagaag gacgatacac tttggtctct tcaacgttgt
    10141 gcatacatac cgggatacgc agcatggagg caagagtgat aaagtctgcg ccaacgtggc
    10201 cgatggtcag aactccgtgg ttggcacccc agttcgccat taccgaatac acatccgtaa
    10261 acggcccttt accagtgagg cgcggtgcaa accaggtggt tggctaggtt gagttggtgc
    10321 gtttgttgag gatgtcatgc acatccttcg gcaattccac gctccagcct tccgcgattt
    10381 gcagtaccgg tcccaggcct ttgatgatgt tgacacgagt catggtgaac gggacgccgc
    10441 cttcggtaag gaagcgggaa gagtaaccgc cgccacggaa gtattcgtgg atcgccgggc
    10501 accattcggt agcggcgagg caagcgtcag cctcttgctg agagatttcc cagtgcggct
    10561 tcatcgtcgg gttaccttcg ctgtcgcgtt gtttacagga accgtccagc gcagcagaac
    10621 ctgagttgat caaatggatg atgccgtgtt ctgccagtcc gtccagttta tgccccgtta
    10681 cacgctcaat tgcttctggt gaccagtagg tacgcacatc ggcaaatacc tgagcggtgc
    10741 cggtgagctg gtgacccatt agcattgcca cgccgttaag actgtcgttt tcggtcgcca
    10801 cgacaaaggg ttcgcgcacg ccattccagt caaatgaact gttgaggatc gcttcggcgg
    10861 tgtcaccatt gggatattga tcggtccagt gacgttgccc ctggaagccc gcagcgatgg
    10921 cgttgtagcc aagtgattct tccacgcgac caatatcggc cagtttgctg ttgccttgca
    10981 tcatgtcgcg gatacacatc gccatcagta aactttcgcg cagaactgcg cggctttgct
    11041 cggcattgcg ttgatactgt ttgttatttt catcttcgcc atagcggaag tttttatcag
    11101 cccaggccag tgccatttcc agttcggctt cgtcgtaaat cttctgatcg atacggcgac
    11161 gcagttcggt catatccacc gcctggactt tcattcccag ccaggattca aagaagttgt
    11221 gatcaacaat agaaccggcg atacccatcg aaacgccgcc cagcgacaga tagcttttac
    11281 ctttcatgct ggcgacggcc aaaccggcgc gggcaaagcg cagcagtttt tcttcaacat
    11341 cggcaggaat cgatgtgtca tcggcatcct gaacgtcatg accgtaaatg gagaatgccg
    11401 ggatgccttt ctggctgtga gctgccagag ccgctgccag gtaaacagcg ccggggcgtt
    11461 cagtgccgtt aaagccccaa atggctttcg ggcgggttgg atccatgtcg atggtttcac
    11521 tgccatagca ccagcaaggc gttaccgtaa tggtgaggcc tacattctgg ctgctgaatt
    11581 tttcttcgca agcagcggct tcagccatac ccgcgataca ggtatcggaa atgacacact
    11641 cgacggcagc tccgcaggca tggcgcagtt tctcggtcag cagtgcggcc gtagctttcg
    11701 ccatattcat tgtttgttct tcaagcgact cacgaacacc catgcgacga ccgtcaataa
    11761 ccgggcggat accaattttc ggtaagctga tttttttcat tacagattcc tcactttatt
    11821 cggaaaatat gttcagttag ttgccgtttg agaacggaaa cgggcaaaga taaagatgac
    11881 cgcgaagcag agtgcgggga tcagttcagc agtggggatg ttgcccgccg cgtcactgac
    11941 aaaacccatg accggagtga caataccgcc gccaataatg gtcataacga tgaaggacga
    12001 accgtatttg gtgtcctggc cgagattctt aatgcccagc gagaagattg ttgggtactg
    12061 aatcgacata aaggcgctgc ataaagtcag ggctattaag cccacatgac cgccagcgaa
    12121 ggctgagatc aggcacagtg ccatagcgat taatgcgtag gcggccagga ctttgtgtgg
    12181 tgcgaagcga ctgatgagcc aggtaccggt gaaacgacca ataaagaagc acaccatggt
    12241 tccggttaaa tagttagcgg caaagcctgc agtcatacct ggaatttctt ctacagcgta
    12301 gcgaatcaaa tagctccagc aggccgtttg tgcgccgaca tagcagaatt gcgctaatac
    12361 cgcccagcgc cagtgacgaa tacgcgccag acgagaaagc gatgcggaga acgatccttg
    12421 tttggcgtca ctgtgattat cactctgcaa tgccgggaat ttcgtcagca tgatcagcag
    12481 ggcgaccagt aacacgatag ccacgatgat catataaggt gtctgtaccg ataataccag
    12541 gctgtgttta tacgcactca attgctctgg agacatttta tcgagaacgt cttgcgattg
    12601 atgtggcacg ttagacaaaa taagactttg cccaaagaca accgcgataa ttgcgccaaa
    12661 cgagttaaat gtttgcgcaa gatttaagcg gaagtgacta ctgctttccg gccctaatac
    12721 cgtaacaaaa gggtttgcgg cagtttccag acaacctaat ccggctgcaa taataaatag
    12781 gccaactaaa aataaggtgt agttcattat ttctgcagcg ggccagaata atgcagcacc
    12841 caaggcatat aaaaataatc cggtaataat ccctgcttta taactgagtt ttttcatcaa
    12901 tatcccagca gggattggga taatgaaata accaaagtaa aaggccgatt ggatcaggcc
    12961 agcctggaaa tttgtcagcg taaaagcctg ctggaattga ggtaataaaa tgtcgttaag
    13021 gttattggct accgcccaaa gaaaaaacag tgagcacagc agcgcgaatg gaataatgta
    13081 acttctgctt tgccctgcat ctttatctac cgcacggtaa ctctgcgttt gtattgatgt
    13141 gtttcccata gcatcctctt aggttcagaa gcttaagcac gtaatcggtt atgttttcaa
    13201 aagtgcaaaa ccagcgagtt cgggcttcat atcggtgaat atttgcctgt ctgaaatgaa
    13261 ctcatggatt cattttcaaa taaaaaacgc tctgtaatat gacggcggtc acacttatta
    13321 gctggaaggt gactttatgt gactaccatc actttaataa tcgaaatttc atgtccgttt
    13381 cagaaaataa atcataaaaa acacaaacca gatgaccgta ttaattgttt gtgccgaaaa
    13441 cgggcatttc tggcgagggg aaatggaaaa atgaccgttt tgataaatat ttttaagagc
    13501 ggtcattttt aacggggtta taaaaagaga acttgcagtt atttactgtg attagttgag
    13561 ctaggtcaca aaatataagc ttgttaattt ctttcttgtt aatcaggaag aagatggtgg
    13621 gtaattaaac ggctaattca atagtgtgaa aggaacaaca ttattgccct gttttgaatc
    13681 agagagaggt agctaatgga acgaaataaa cttgctcgtc agattattga cacttgcctg
    13741 gaaatgaccc gcctgggact gaaccagggg acagcgggga acgtcagtgt acgttatcag
    13801 gatgggatgc tgattacgcc tacaggcatt ccatatgaaa aactgacgga gtcgcatatt
    13861 gtctttattg atggcaacgg taaacatgag gaaggaaagc tcccctcaag cgaatggcgt
    13921 ttccatatgg cagcctatca aagcagaccg gatgccaacg cggttgttca caatcatgcc
    13981 gttcattgca cggcagtttc cattcttaac cgatcgatcc ccgctattca ctacatgatt
    14041 gcggcggctg gcggtaattc tattccttgc gcgccttatg cgacctttgg aacacgcgaa
    14101 ctttctgaac atgttgcgct ggctctcaaa aatcgtaagg caactttgtt acaacatcat
    14161 gggcttatcg cttgtgaggt gaatctggaa aaagcgttat ggctggcgca tgaagttgaa
    14221 gtgctggcgc aactttacct gacgaccctg gcgattacgg acccggtgcc agtgctgagc
    14281 gatgaagaga ttgccgtagt gctggagaaa ttcaaaacct atgggttacg gattgaagag
    14341 taatttcgta aagcaacaag gagaaggatg atggctaaca gaatgattct gaacgaaacg
    14401 gcatggtttg gtcggggtgc tgttggggct ttaaccgatg aggtgaaacg ccgtggttat
    14461 cagaaggcgc tgatcgtcac cgataaaacg ctggtgcaat gcggcgtggt ggcgaaagtg
    14521 accgataaga tggatgctgc agggctggca tgggcgattt acgacggcgt agtgcccaac
    14581 ccaacaatta ctgtcgtcaa agaagggctc ggtgtattcc agaatagcgg cgcggattac
    14641 ctgatcgcta ttggtggtgg ttctccacag gatacttgta aagcaattgg cattatcagc
    14701 aacaacccgg agtttgccga tgtgcgtagc ctggaagggc tttccccgac caataaaccc
    14761 agtgtaccga ttctggcaat ccccaccaca gcaggtactg cggcagaagt gaccattaac
    14821 tacgtgatca ctgacgaaga aaaacggcgc aagtttgttt gcgttgatcc gcatgatatc
    14881 ccgcaggtgg cgtttattga tgctgacatg atggatggta tgcctccggc gctgaaagcc
    14941 gcgacgggtg tcgatgcgct cactcatgct attgaggggt atattacccg tggcgcgtgg
    15001 gcactaaccg atgcactgca cattaaagcg attgaaatca ttgctggggc gctgcgagga
    15061 tcggttgctg gtgataagga tgccggagaa gaaatggcgc tcgggcagta tgttgcgggt
    15121 atgggcttct cgaatgttgg gttagggttg gtgcatggta tggcgcatcc actgggcgcg
    15181 ttttataaca ctccacacgg tgttgcgaac gccatcctgt taccgcatgt catgcgctat
    15241 aacgctgact ttaccggtga gaagtaccgc gatatcgcgc gcgttatggg cgtgaaagtg
    15301 gaaggtatga gcctggaagg ggcgcgtaat gccgctgttg aagcggtgtt tgccctcaac
    15361 cgtgatgtcg gtattccgcc acatttgcgt gatgttggtg tacgcaagga agacatttcg
    15421 gcactggctc aggcggcact ggatgatgtt tgtaccggtg gcaacccgcg tgaagcaacg
    15481 cttgaggata ttgtagagct ttaccatacc gcctggtaaa tgcgctgatg tgataatgcc
    15541 ggatacgatt tgcggcgtat ccggctcgcc gttaccgtac caaccgcaat tgctgtaaat
    15601 tgccgtcgat atgcaaatcg gtttgtaagc gggcaatatc gcggcacaga aacgccatct
    15661 ctttatgggt ttctaatttt ttgcgccact tttcggcaac cgcatccaga ttctcatata
    15721 tcccttccag actctgaaac tcgaccagca gctgcgtggc gctttttggt ccgattcccg
    15781 caacacccgg tacctttgaa ctgctgatcc ccgcaagtcc ccagtaatcg ggcaactgct
    15841 gcggttgaac gccaaattct ttatcgataa atggcgcatc cagccaacgt ttctggaagt
    15901 aatcacgaat acgtaatgtc ggtgaaagta actgacagta gcctttatct gtcgaaacaa
    15961 tcgttgcctg atgcccggcc tgtgtcactt tgaccgccag cgtggcggct aagtcatcgg
    16021 cttcgttgcc gctggctgac cagcacggga cgccgcgttg ctcaaaggcg gcgcgtaatg
    16081 caggcatctc gtcgtgcaac tcttccggca ttggcggtcg atccgctttg taatctggta
    16141 aacgctgatg acgccagccg ctactgcggt tttcatcatc aaaaacggcg accgcgtggg
    16201 ttggctggct gtgcataatg agctgatcga gcgcatgctg gcaggtttcg acacagggcg
    16261 aaccctgaac ggcatgaatg cgacggataa gattcagtgc atcgacaata agcaaatgaa
    16321 cagccacgat aaccccctta aagatcaggc gataagggta acattcgatg ccggaacagg
    16381 ctatggctgg ttgagaaaac aggaagaggc ctcgcaaaac gaggcctctg ggagagatta
    16441 atcgcaggca acgatcttca tcgccaggcc gccgcgagag gtttcgcggt acttggcgtt
    16501 catgtcttta cctgtttcgt acatggtttc gataacttta tcgaggcaga cgcgcggttc
    16561 gctggtacgg cgcagtgcca tacgcgcggc gttcaccgct ttcactgctg caatggcgtt
    16621 acgctcaatg cacggtacct gaacctgccc cgcgaccggg tcacaagtca gaccgaggtt
    16681 gtgctccatc gcgatttccg ctgcgatgca aacctgcgcc gggcttgcgc ctaacagttc
    16741 tgccagacca gctgccgcca tagagcacgc cacgccaact tcaccctggc aacccacttc
    16801 ggcaccagaa atcgacgcgt tcatcttata aagagaacca atggcgctgg ctaccagcag
    16861 gtaacgagcc agtgagttag cgttcacttc gcggataaac ttatcgtagt acgccagcac
    16921 agccgggata atcccgcacg caccgttagt cggcgcagtc accacgcgac cgccagcagc
    16981 gttctcttcg ttcactgcca gtgcaaacat gttgatccag tcaacaaccg ccatcgggtc
    17041 agtggtggtt ttatcctggc tgaccagcat ccggcgtagt gccgcagcac ggcgtggaac
    17101 gcgcagtttg ccaggcaaca cgccttcggt ggaaataccg cgctcaatac cgccacgcat
    17161 gacttcccag acgttcgcca ggtgctgttc cagctcttct ttactgtgca gcgccagctc
    17221 gtttttcatc atcaggccag agagtgacag cccggtttcc tgacaatgtt tttgcagatc
    17281 ggctgctgaa ctgtacggat aaggaacttc aaccggtgcg ctatcctgct ggccaaaatg
    17341 ctcttcatca acgataaagc caccgccaat agagtagtaa gtctggctgt aaacgacttt
    17401 atcgcccgcc agcgcggtaa tgcgcatacc gttttcatgc agagaaaggt tgtctgcatg
    17461 gaagttcatg cactgatcaa ccgggaactc cacttcatgc tgaccgtttg ccagcatcag
    17521 gcgaccatga gtattcacat cctgaataaa accggggatg gaatcgatat ccacggtatc
    17581 cggcaggtta cccgctaggc ccataataat ggcgatatct gtgtggtggc ctttaccagt
    17641 cagagagagc gagccgtaca cgtcaaccac cacgcgggtc acgtctttaa gcaggttacg
    17701 ggcaatcaga tcgtcggtaa attgtttacc cgctttcatt ggtccaacgg tatgagaact
    17761 ggaagggcca atgccgattt tgaaaatatc gaatacgcta atcataggaa atacatcgcg
    17821 ttaaaacgga ggaagcgccg cccgaaagcg gcgcgaaagg acttagctga acagagagta
    17881 gaagattgcg gagattgcaa tcagacccat cacgacaacg aatacgttgc tgatgtgacc
    17941 gctgtactta cgcattgccg gtactttctg aattgcgtac atcggcatca ggaacaggat
    18001 catcgcgatg attgggccgc ccagggtttc aatcataccc aggatgctcg ggttcagggt
    18061 ggcaacaatc caggtcgtta ccagcatgaa cagcgcagtg atacggttca gcttgttgat
    18121 ttcgatagac ttacctttac cacgcagaga tttaatcacc ataccgttga agccttcacg
    18181 tgcgcccagg tagtggccga ggaaggattt ggtgatagcg ataatcgcga taatcggagc
    18241 catccacgcg ataaccggtg cgttaaagtg gttagccagg taagacagaa tcgagatgtt
    18301 ctgctcttta gccgcagcca ggtctgccgg agtcaggctc agtacgcagc tgaagacgaa
    18361 gaacattacg gtcagcacca tcatgatgtg tgcgaatgcc aggatcttcg agcatttctg
    18421 ttctgccata tcgccgtact cttcacgctt cgcaacggcg aaagaagaga tgatcggaga
    18481 gtggttgaac gagaacacca ttaccggaat tgccagccac agggtcatcc acagaccgtt
    18541 tccggttgca gatgcagtgt ccagagacag cgtttccagt gctgcgccgt tccactgcgg
    18601 gatcaggtac agagccagca gcatcagtac gccaacaaac gggaatacca gaatactcat
    18661 cgctttaacg atcatctgct caccaaagcg aacgatggtc atcataccca cgatcaggat
    18721 cagcgacaga atcgcacgcg gcggtggcgt catacccagc tggtgagaca tgaagctttc
    18781 aacggtattg gtgattgcca cgctataaac cagcaggatc gggtagatag cgaagaagta
    18841 gagcagggta atcagtttac ctgcgccaat accaaagtgt tcttctacaa cctcggtgat
    18901 gtcttcgccc gggtttttac cagacagtac gaagcgagtc aggccgcggt gagcaaaaaa
    18961 cgtcatcggg aacgcaagga tagccatgat gatcagcggg atcataccgc caacaccggc
    19021 gttgattggc aggaacagca cgcccgcgcc gattgccgtg ccgtaaaggc ccagcatcca
    19081 catggtgtct gtcttgcgcc aggcactacg agagtctttc gacgcaatcg tgctggtttg
    19141 agtcgtttcc atctatttct cctggaggaa tgcaaataat tcagctttta gccagttcag
    19201 cccgggaagg tgcctgaccg taaaaatgaa atctgtctat cggcgatggc ttaatcattt
    19261 accgccgtaa taatttgagg gcggaaagat acacacaagt tatgtatcta tcagtgatct
    19321 tgatcccaaa tacaataatc tacttggaag atagattatc ccaacgcttt actctgtaaa
    19381 tcaaaagtaa aagagaattt aagggcaata tttgcccatt aaacgacatc tgacttcttc
    19441 cggggcgcgc aatatatgta ggttgaactg aaaaaatcaa tacttttagt taatcatggc
    19501 gataatttct ttttcatagc gataaaatac ggtttatgaa aatagggaaa cgtttgcgtt
    19561 gcaacataaa ttgtatgaca tattaactgt gatgatattt gtcgcgtgag ggtggtaaga
    19621 aacattcata taacaaatga aatgagttga tgagtaagtg accaaaaacg agaaaacccg
    19681 gcaacaaacg tcaccgg
//
LOCUS       DADLSA010000072        19368 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.072, whole genome shotgun sequence.
ACCESSION   DADLSA010000072 DADLSA010000000
VERSION     DADLSA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.072"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="JN691_002466"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="JN691_002466"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAY7243886.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="JN691_002467"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="JN691_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAY7243887.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3744..4163)
                     /gene="atpC"
                     /locus_tag="JN691_002468"
     CDS             complement(3744..4163)
                     /gene="atpC"
                     /locus_tag="JN691_002468"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAY7243888.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4184..5566)
                     /gene="atpD"
                     /locus_tag="JN691_002469"
     CDS             complement(4184..5566)
                     /gene="atpD"
                     /locus_tag="JN691_002469"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAY7243889.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5593..6456)
                     /gene="atpG"
                     /locus_tag="JN691_002470"
     CDS             complement(5593..6456)
                     /gene="atpG"
                     /locus_tag="JN691_002470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAY7243890.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6507..8048)
                     /gene="atpA"
                     /locus_tag="JN691_002471"
     CDS             complement(6507..8048)
                     /gene="atpA"
                     /locus_tag="JN691_002471"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAY7243891.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8061..8594)
                     /gene="atpH"
                     /locus_tag="JN691_002472"
     CDS             complement(8061..8594)
                     /gene="atpH"
                     /locus_tag="JN691_002472"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAY7243892.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8609..9079)
                     /gene="atpF"
                     /locus_tag="JN691_002473"
     CDS             complement(8609..9079)
                     /gene="atpF"
                     /locus_tag="JN691_002473"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAY7243893.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9141..9380)
                     /gene="atpE"
                     /locus_tag="JN691_002474"
     CDS             complement(9141..9380)
                     /gene="atpE"
                     /locus_tag="JN691_002474"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAY7243894.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9427..10242)
                     /gene="atpB"
                     /locus_tag="JN691_002475"
     CDS             complement(9427..10242)
                     /gene="atpB"
                     /locus_tag="JN691_002475"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAY7243895.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10251..10631)
                     /gene="atpI"
                     /locus_tag="JN691_002476"
     CDS             complement(10251..10631)
                     /gene="atpI"
                     /locus_tag="JN691_002476"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAY7243896.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11248..11871)
                     /gene="rsmG"
                     /locus_tag="JN691_002477"
     CDS             complement(11248..11871)
                     /gene="rsmG"
                     /locus_tag="JN691_002477"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAY7243897.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11935..13824)
                     /gene="mnmG"
                     /locus_tag="JN691_002478"
     CDS             complement(11935..13824)
                     /gene="mnmG"
                     /locus_tag="JN691_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAY7243898.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14203..14646)
                     /gene="mioC"
                     /locus_tag="JN691_002479"
     CDS             complement(14203..14646)
                     /gene="mioC"
                     /locus_tag="JN691_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAY7243899.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14736..15194)
                     /gene="asnC"
                     /locus_tag="JN691_002480"
     CDS             complement(14736..15194)
                     /gene="asnC"
                     /locus_tag="JN691_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAY7243900.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15346..16338
                     /gene="asnA"
                     /locus_tag="JN691_002481"
     CDS             15346..16338
                     /gene="asnA"
                     /locus_tag="JN691_002481"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAY7243901.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16343..17794)
                     /gene="viaA"
                     /locus_tag="JN691_002482"
     CDS             complement(16343..17794)
                     /gene="viaA"
                     /locus_tag="JN691_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HAY7243902.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17788..19284)
                     /gene="ravA"
                     /locus_tag="JN691_002483"
     CDS             complement(17788..19284)
                     /gene="ravA"
                     /locus_tag="JN691_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAY7243903.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELQEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4619 a         5126 c         4857 g         4766 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca attgaccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc aacagttcgt tgttcggtgc
      301 aacgacgata accggcatat ccgcgtcaat cagcgccagc gggccgtgtt tcagctcgcc
      361 tgcagcgtag gcttcagcgt gaatgtaaga gatctctttc agcttcaatg cgccttccag
      421 cgcgattggg tactgatcgc cacggcccag gaacagcgcg tgatgtttgt cagagaaatc
      481 ttctgccaga gcttcaatgc gtttatcctg agacagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttta tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcatcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaggccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
     1441 cccgccttgt ttcagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccattatgc accaccacaa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatgca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcgag tcatatgacc
     1741 ttccgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgcccg gctttcgtgc ctttacccag acgcgctttt
     2341 ttcatctcaa cgaagttacc aacgtgtgca ccttccagca actcagcacc aggacgcaga
     2401 cgggcaaacg ggccaatagt acaggccgct gccagattcg cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttggc aatcagaatg
     2881 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctggggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaggtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg gttcaacgcg taaaaaaaag ccagcctgtt tccagactgg
     3721 cttttgtgct tttcaagccg gtgttacatc gcttttttgg tcaactcgat aacgcgcagc
     3781 tgcgcgatcg ctttggccag ttccgcagac gcctgagcgt aatctacgtc gccgtgagag
     3841 ctgctaatgt gctcttcagc cttacgtttc gcttccatgg ctcgcgcttc gtcgagatcc
     3901 tggccgcgaa ttgcggtgtc ggccagaacg gtcacgttgc caggctgcac ttcaagaatg
     3961 ccgccagaca gatagataaa ctcttcgtga ccgtgctgtt tcacgatgcg aatcatacca
     4021 ggcttaatgg cggtgagcag cggtgcgtgg ccagggtaga tccccagttc accttcgcta
     4081 cccgttacct ggattttctc gaccagacca gagaacattt gttgctctgc gctgacgacg
     4141 tccaggtggt aagtcattgc catatcaccc tccgattaag gcgttaaagt tttttggctt
     4201 tttccacagc ttcttcgatg gaaccgacca tgtagaacgc ctgctccggc aggtgatcgt
     4261 attcgccttc catgatgcct ttaaagccac ggatggtgtc tttcagggag acgtatttac
     4321 ccggagaacc ggtgaatact tctgccacga agaacggctg ggacaggaag cgctggatct
     4381 tacgagcacg cgctaccacc agtttgtctt cttcagacag ttcatccata cccaggatgg
     4441 cgatgatgtc tttcagttcc tgataacgtt gcaggatgga ctgaacgcca cgcgcggtgt
     4501 cgtagtgttc ctgaccaacc accagcgggt ccagctgacg gctggtggag tccagcgggt
     4561 caacggccgg gtagataccc agagacgcga tctgacggct cagtaccacg gttgcgtcaa
     4621 ggtgcgcaaa ggtggttgcc ggagacgggt cagtcaagtc atccgcaggt acgtataccg
     4681 cctgtacgga ggtgatagaa ccagttttgg tggaggtgat acgttcctgc agaacgccca
     4741 tctcttccgc cagggtcggc tgataaccta ccgctgaagg catacggccc agcagtgcgg
     4801 atacttccgt accggccagg gtgtaacgat agatgttgtc aacgaacagc agaacgtcac
     4861 gaccttcgtc acggaatttc tcagccatgg tcagaccggt cagagcaacg cgcagacggt
     4921 ttcccggcgg ctcgttcatc tggccataca ccagggatac tttgtcgata acgttggagt
     4981 cggtcatttc gtggtagaag tcgttaccct cacgagtacg ttcacctacg cccgcaaaca
     5041 cagagtaacc ggagtgctcg atcgcgatgt tacgaatgag ctccatcatg tttacggttt
     5101 tacctacacc cgcaccaccg aacagaccaa ctttaccgcc cttagcgaac ggacacatca
     5161 ggtcgataac tttgataccg gtttccagca gttcctgaga gtttgacagc tcttcgtagg
     5221 aaggtgctgc gcggtgaatc gcccaacgct cttcttcacc gatctcgcct ttcatgtcga
     5281 ccggttcacc cagtacgttc atgatacggc ccagagtcgc tttacctacc gggacttcga
     5341 tcgggtgttc gaggtctttt acatccagac cgcgacgcag accgtcggag gaacccattg
     5401 cgatggtacg cacgataccg ccgccgagct gctgctgaac ttccagcacc agacgctcat
     5461 taccattttg cacctcaaga gcatcgtaca cgcgcggtac ggcatcctga gggaattcga
     5521 cgtcaactac ggcgccgatt acctggacaa tctttccagt agccatctta aatcctctac
     5581 gaaataacct gtttaaaccg cggcggcccc cgagacgatc tcggtgagtt cctgagtaat
     5641 gctggcctga cgagctttgt tgtataccaa ctgcagctct ttaatcaggc tgccgccatt
     5701 gtcggtcgcg gctttcatcg ccaccatacg ggcggcctgc tcgctggcca ggttttcaac
     5761 cacgccctga taaacctgag attcgacata acgacgcagc agggtatcca gcaacgcctt
     5821 cggatcgggt tcgtacaggt aatcccagga tttatgtttc agatcatcat catctgatgc
     5881 cggtaacggc agcagctggc tgatggtcgg aacctgagac atggtgttaa taaatttgtt
     5941 gctgacaatg taaagcttgt ccagacggcc ttcgtcgtag gcctgcaaca tcacttttac
     6001 cggaccgatc agttcggaca gggaagggtt atcccccatg ccggtgacct gggcaacaac
     6061 attgccgccc acggagttga agaacgacac gcctttcgag ccgatcattg cgaggtcgca
     6121 ttgaacgcct ttgtcggtcc aggtcttcat ttccgccagc agttttttga acaggttaat
     6181 gttcaaacca ccgcacaaac cacggtcggt cgacaccacc aggtagccca cgcgtttaac
     6241 gtcgcggtct tccaggtaag ggtgcttata ttccagatta ccgtgtgcaa ggtgaccaat
     6301 cactttgcgc atggtttctg cataaggacg gctggccgcc atgcgatcct gcgatttacg
     6361 cattttggaa gcggcgacca tctccatcgc tttagtgatc ttttgcgtgt tctggacgct
     6421 tgcgatctta ctacgtatct cttttgcgcc ggccatgagc ttctcctcaa tgccttgcgg
     6481 cctgccctaa ggcaagccgc cagacgttac caggattggg ttgctttgaa ggaatcgagg
     6541 atgcctttca gcttgccttc gatttcgtcg ttgtagccac cggtctggtt gatctcttgc
     6601 atcaacggag cgtgatcacg gtcgacgtaa gccagcagag cggcttcgaa gctgccaatt
     6661 ttcgacagtt caacatccgc caggtaacca cgttctgctg cgaacagaac cagagactgc
     6721 tgcgcaacgg acatcggcgc atactgtttc tgtttcagca gttcggtcac tttctgaccg
     6781 tggtcaagct gcttacgtgt tgcatcgtca aggtcggatg caaactgaga gaacgctgcc
     6841 agttcacgat actgtgccag agcggtacgg ataccaccgg acagtttttt catgatcttg
     6901 gtctgtgctg caccaccaac acgggatacg gaaatacccg ggttaaccgc aggacgaata
     6961 ccggcgttga acaggttggt ttccaggaag atctgaccat cggtaatgga gattacgttg
     7021 gtcggaacga acgcagaaac gtcacccgcc tgagtttcga taatcggcag tgcggtcaga
     7081 gaaccggttt tccctttcac ttcacctttg gtgaaggctt caacgtattc ggcgttaaca
     7141 cgtgcagcac gctccagcag acgagagtgg aggtagaaaa cgtcgcccgg gaatgcttca
     7201 cgtcctggcg gacgacggag cagcagggag atctgacggt aagcaacagc ctgtttagac
     7261 aggtcatcgt aaatgatcag cgcatcttca ccgcggtcac ggaagtattc gcccattgcg
     7321 caaccggcat acggtgccag gtattgcagt gcagcggatt cagacgcggt tgctaccaca
     7381 acgatggtgt tagccagtgc gccgtgctct tccagtttac gtaccacgtt agaaatggtg
     7441 gacgctttct ggccgatagc gacatagata catttgatac cggaatcgcg ctggttgatg
     7501 atggcatcga tagccagtgc ggttttacct gtctgacggt caccgatgat caattcacgc
     7561 tgaccacgac cgattgggat catggagtca acggctttat aaccggtctg taccggctga
     7621 tctacggact gacgttcgat aacgcccgga gcgattgctt ctacagcaga gaagccgtcg
     7681 tgatccagcg gacctttacc gtcgattggt gcacccagag tgttaaccac acggcccagc
     7741 aggccacggc caaccggaac ttccaggata cggccagtac acttaacttt catgccttcg
     7801 gcaaggtcag cgtacggacc cataacaacc gcacctacag agtcgcgctc gaggttcagt
     7861 gcgatagcgt aacggttacc cggcagggag atcatttcac cctgcataca atcggccagg
     7921 ccgtgaatgc ggataacacc gtcacttaca gaaacaatag taccttcgtt gtgagcttca
     7981 ctcacaacat tgaactgagc aatgcgctgc ttgatcagtt cgctgatttc ggtggaattc
     8041 agttgcatgc tccagtcccc ttaagactgc aagacgtctg caaggcgctc aagacgaccg
     8101 cgtacgctgc catcaatgac catatcaccc gctcggatga taacgcctgc cattacagac
     8161 ttatcgattt tgcaattcag cttaactttg cgtgacagac gtttttccat cgcagcagaa
     8221 attttcgcga gctgttgttc actcagtgcg gcagcggaaa tgacgtctac ctcagcggta
     8281 gcctcactca cggcacgaag gtgaataaac tgctccagaa catccgggag cgcgttaaga
     8341 cgaccatttt cagccattac ccgaatcagg ttctgaccgt tttcgtccag ttgctcacca
     8401 caaactgcga taaacgactc ggcgagcgtt tctggcgcaa gcgcgccaga gagaagctct
     8461 gccatttgtt cgtttttggt tacctcggcg gcaaacgcca gcatgtcctg ccagcgttct
     8521 acactttggt gttcgacggc aaagtcaaaa gctgctttgg cgtaggggcg agctaccgta
     8581 ataaattcag acatcagccc ctccctcctt acagttcagc gacaagttta tccacgatgt
     8641 cgctgttagc agcttcatcc acggaacgtt cgatgatctt ctcggcgcca gcaacagcca
     8701 ggatagcaac ttgcttacgc agctcttcac gggcacgttt acgctcggct tcaatttctg
     8761 cctgcgcctg ggccacgatt ttagtacgtt cctgttctgc ctcagctttc gcttcgtcca
     8821 gaatctgcga gcggcgtttg ttcgcctgct cgatgattac ctgggcttcc gctttcgctt
     8881 ttttcagctg gtcggtcgcg ctggcctttg caaggtcaag gtccttatgt gctcgttctg
     8941 cggaagcaag gccgtcagca atttcttttt gacgtttttc gatggctgcc attaatggcg
     9001 gccatacgta cttcatgcag aacagaacga acaggacaaa cgcgatggcc tggccgagga
     9061 ttgttgcgtt aagattcaca gcacaatgcc tctatttagt taacgttctg atattgctct
     9121 ttaaataaaa gcaacgctta ctacgcgaca gcgaacatca cgtacagacc cagacctaca
     9181 gcgatcatcg ggatagcatc caccagaccc ataacgataa agaactgagt acgcagcaga
     9241 ggaatcagat caggttgacg cgctgcgcct tccaagaatt tacccccgag gatgccgata
     9301 ccgatcgcag caccgattgc cgccagaccc atcatcacag cggcagccat gtacagcaga
     9361 tccatattca ggttttccat gacagtctcc agtttgtttc agttaaaacg tagtagtgtt
     9421 ggtaaattaa tgttcttcag acgccatcga cagatagacg atcgtcagaa ccatgaagat
     9481 gaaggcttgc agcgtaatga tcaggatgtg gaaaatggcc cacggcacat tcaggatcca
     9541 ctgtgaccac cacggcaaca gaccagcaat cagaatgaaa atcagctcac cggcatacat
     9601 gttaccgaac agtcgcaaac cgagtgaaac tggtttggac agcaggctta ccccttcaag
     9661 gattaagttg acaggaatga acgcccagtg attgaacggc tgcagcgtca actctttcgt
     9721 gaagccgccg atgcctttca ttttgatgtt gtagaacaga atcaggataa atacgcccag
     9781 tgccatagac agcgttacgt tcacgtccgc agacggaacc acacgcagtg caggcagacc
     9841 cagtacatgt tcagcaatgt acggcagcag gtcgataggc agtaaatcca tcaggttcat
     9901 caggaatacc cagacgaaga tcgtcagggc cagcggagca atcagcttgc ttttgccatg
     9961 gtacatgtct ttcacgctac cattaacaaa gccgatcacc agctcaatcg cggtctgaaa
    10021 cttacctggc acaccgctgg tcgccttttt ggctacgcta cggaataaaa ccaggaacaa
    10081 cagacccagc accaccgaga agaacatgga gtcaatattg attgtccaga aggtggctgg
    10141 ggggttttgt ggatccacca gcgagaatgt acgcaggtcc agctgaaggt tattcaggtg
    10201 gtgtcctatg taatcctgcg gcgtcatatt ttctgaagcc atgatgcctt ttaccctttg
    10261 ttgttaatta cagccggtgc cagtatctga accaccagca ccaaaaccca cgtaacgatc
    10321 agcggcaaga ataccgcctt taaaaccgcc aacgccacca ccagtaacac caacatcgcc
    10381 agaactttga aagcttcgcc aaatgcgaat gtccaagcca cccggccttt cgctggtgta
    10441 tgcgcctggt gacgccaggc aaatatcata aacaaaacgt taggcagaaa gactgccagg
    10501 cccccgctta ttgcagagac gccccagaag gggtctttga ggctgaacag caatccactt
    10561 gctatcacca ccagtaactg aacgagcaga agcttccgag caacgtttcg actcacgagc
    10621 gacacagaca tcacgttttt cactcctgct cccttcgagg tatgccgcgt gtcgtataaa
    10681 actttcttta aggcttagag tcaagcatca aaaagcggtc aaattatacg gtgcgccccc
    10741 gtgatttcaa acaataagta gccaaaaggt gaataaatgt ttaaatattt ttccagtgca
    10801 tacaattgcg acttttccgc taaccctgtt cgatcatgaa aaactgtaaa taacgcgtaa
    10861 acactggtga taaagcgtgc ttcagatcac atattgcgca tgttcgcgca cagcatattt
    10921 atttacttgg caaatgatgc ctttgcaagt ttatgatatt tcagtctaaa aacagatact
    10981 gttttaataa atgacattta cacaacaaaa accacccatt gacattttta ataatgtttt
    11041 aacagccaat gatggttctt agcgccgatt tttagcagac tgatattttc actaatgact
    11101 tattttctgc ttaccaaaaa aagccacgtt atcttgttga tgcaaaagag tgaacgtggc
    11161 gttaaatgta accagttata tcagtagaaa acctggttgt tgttaacagt ctaaccggtc
    11221 aattttttat gatttttttg ataaaaatta aattttattt gctttaatca ccaccagatg
    11281 acgttcgcca tccagggctg gaacctgaag tttaaccact gattcgacct gatattcttc
    11341 gggcaacaaa gcgatttcat cttccggcat ttgccctttc agcgcgtaga aacggccttg
    11401 ctcaccagga agatggtggc accagctcac catatcgttc agagaggcaa aagcgcggct
    11461 aattacgcca tcaaatggcg gctctgaagg aaactcttct accctgctct gtactggttc
    11521 aatattctcc agtttaagct catgttgcac ctgacgaagg aaacgcacgc gtttaccaag
    11581 gctatccaac agagtgaaat gggcttcagg acgcacgata gagagtggaa tgcctggcag
    11641 tcctggtccg gtgccgacat cgataaaccg ttcaccttgc agatacggtg ccaccacaat
    11701 gctatcgaga atatggcgta ccagcatctc attaggatcg cggaccgaag tcaggttgta
    11761 cgctttgttc catttatgca gcatattcac gtaggcaata agctggtttt tctggtgatc
    11821 agtaagcgaa atacctgcgt ctttcagcag taaggagagt ttgttgagca cggtgattac
    11881 ctgttcttga tgcgttgcct ggtaagcggg tgcttaccag gcatttttaa tgcgttatgc
    11941 gctacgacgc agcataccct gttttttcag ccacaccagc agaatggaga tggccgcagg
    12001 cgtgacgcca gaaatacgcg aagcctggcc gatagaggcc ggtttgtgat cgttaagttt
    12061 ggcgatcact tcgttagaaa gaccggatac ctggcggtaa tccagtgtcg cgggtagcag
    12121 ggtgttctcg ttacgcagct gcttttcgat ctcatcttgc tggcgcgcga tataaccttc
    12181 gtatttaacc tgaatctcaa cctgttccgc cgcctgttcg tctgtcaacg caggggcaaa
    12241 cggcgtcagc gtggttaatt tttcataagt catttccgga cgacgcagca gatcttcacc
    12301 actggcttca cgggaaagcg gcgcagtcag gtgagcattc acttcggctg cagcttccgc
    12361 cgacggggtt acccaggtcg atttcagacg ctgacgctca cgctcgatat tctcaagttt
    12421 ctcgttaaag cgcgcccaac gttcgtcatc caccaggccc agttcacgac cgatttcagt
    12481 caaacgcaga tccgcattat cttcgcgcag catcagacga tattctgcgc gcgaagtaaa
    12541 catacgatac ggttctttgg ttcctaaagt gcacaggtca tcaaccagta cgccgagata
    12601 cgcctgagaa cgtgccggag cccaaccttc tttgtcagca gacagacggg cagcgttaag
    12661 accggccagc aaaccttgcg cagcggcttc ttcgtaaccg gtagtgccgt taatctgacc
    12721 agcaaagaac agcccctgga taaacttgct ctccagcgtc ggtttcaggt cgcgaggatc
    12781 gaagaagtca tactcaatgg cataacccgg acgcacgatc ttcgcgtttt ccatcccctg
    12841 catagagcgg acgatttgca tctgcacatc gaacggcagg ctggtggaga taccgttcgg
    12901 ataaatttca ttagaggtca gtccttccgg ttcaaggaag atctgatgct gatttctgtc
    12961 tgcgaagcgc atgactttgt cttcgatcga cgggcagtag cgtgggccga caccttcgat
    13021 cacccctgca tacattgggc tacgatcgag gttactgcgg atcacatcat gggttttctc
    13081 gttggtatga gtgatataac acggcacctg ctggggatgc tgggacgcat tgcccataaa
    13141 cgagaatacc ggcattgggt tatcgccatg ctgttgcgcc agcacgctaa agtcgatggt
    13201 tcgagcatca atacgcggcg gagtaccggt tttcagacga ccaacgcgca gcggcagttc
    13261 acgcaaacgg cgagagagcg gaatggatgg cggatcgcca gcacggccac cgctgtaatt
    13321 atccagaccg atatgaattt tgccgtcgag gaacgtccca acggtgagca ccacagcttt
    13381 ggcacggaac ttcagtccca tttgggtaac agcaccgacc acgcgatcgt tttcgacaat
    13441 aagatcttca accgcctgct ggaagatcat caggttcggt tggttctcca gcgccgtacg
    13501 taccgcctga cggtagcgca cacgatccgc ctgagctcgg gtagcgcgaa ccgccggtcc
    13561 tttgcttgcg tttagtatcc taaactggat acccgcttga tcgatcgctt tcgccatcag
    13621 accgccgagt gcatccactt cttttaccag atgtcccttc ccaataccgc cgatcgccgg
    13681 gttgcagctc atctgcccca gagtgtcgat attgtgtgtc aaaagcagag tctgttgacc
    13741 catacgcgcc gcagccatcg cggcctcggt gcctgcatga cccccgccaa tgatgatgac
    13801 gtcaaaagga tccggataaa acatggtgat tgcctcgcat aacgcggtat gaaaatggat
    13861 tgaagcccgg gccgtggatt ctactcaact ttgtcggctt gagaaagacc tgggatcctg
    13921 ggtattaaaa agaagatcta tttatttaga gatctgttct attgtgatct cttattagga
    13981 tcgcactgcc ctgtggataa caaggatccg acttttaaga tcaacaacct ggaaaggatc
    14041 attaactgtg aatgatcggt gatcctggac cgtataagct gggatcagaa tgaggggtta
    14101 tacacaactc aaaaactgaa cagcggttgt tctttggata actaccggtt gatccaagct
    14161 tcctgacaga gttatccaca gtagatcgca cgatctgtat acttatttga gtaaattaat
    14221 ccacgatccc agccattctt ctgccggatc ttccggaatg tcgtgatcaa gaatgttgat
    14281 cttcagtgtt tcgcctgtct gttttgcacc ggaatttttg agttctgcct cgagtttatc
    14341 gatagcccca caaaaggtgt catattcacg actgccaata ccgattgcgc caaagcggac
    14401 cgcagaaaga tcgggcttct gttcctgcaa tgcttcatag aaaggagaaa ggttgtccgg
    14461 aatatctccg gcaccgtggg tggagctgat aaccagccag atccccgagg cagataaatc
    14521 ttccaacagc ggaccgtgca gcgtttcggt ggtaaaaccc gcctcttcca gcttttcagc
    14581 caggtgttct gctacatatt cggcaccgcc gagggtgctg ccgctgataa gagtgatatc
    14641 tgccataaac cgccaccttt attaagagtg gcgtattgta cgctgtgaac gcgttgggat
    14701 ctacctgtgg aaaagtatgg gattaaaaaa gctgatcagg gcttgatggt acgcatgatc
    14761 gggttttgca ggacgatcaa tgtctcggtg gactgaattt catcaattgt ttggatcttg
    14821 ttgataagta catgctggag agcgtcgatc gaacggcaca taacttttat aaagatgctg
    14881 tagtggccgg ttgtgtaata ggcttcagtg acttcatcaa ggctttccag ctttgccagc
    14941 gcggaagggt agtctttggc gctctttaat ataatgccga taaagcagcc tacgtcataa
    15001 ccgagctgct tcgggctgac atcaatacgc gccccggtaa tgatccccgc ctgcttcatt
    15061 ttctctactc gaacgtgaat cgtccccgga ctgacgccaa attgtttcgc cagttcggcg
    15121 taagcggtgc gcgcattgcc cattaatgct tccaggatgc cacggtccag attgtcgatc
    15181 agataatttt ccataggatt ttcttatgca gattgatgat tcattctatt ttagccttct
    15241 ttcttaatga atcaaaagtg tgttaggctt tttattgaat gattattgca tgtgtgtcgg
    15301 tttttgttgc ttaatcataa gcaacaggac gcaggagtat aaaaaatgaa aaccgcttac
    15361 attgccaaac aacgccaaat tagcttcgtg aaatctcact tttctcgcca actggaagaa
    15421 cgtctggggc tgatcgaagt ccaggcgccg attcttagcc gtgtggggga tggcacgcag
    15481 gataacttgt cgggctgtga aaaagcggtg caggtaaaag tgaaagctct gcctgatgcc
    15541 cagttcgaag tggttcattc actggcgaag tggaaacgtc agaccttagg gcaacacgac
    15601 ttcagcgcgg gcgaagggct gtacacgcac atgaaagccc ttcgccccga tgaagaccgt
    15661 ctttctccgt tgcactcggt ctatgttgac cagtgggact gggaacgcgt aatgggcgac
    15721 ggtgagcgtc aattctcgac tctgaaaagc acggtagagg cgatctgggc gggaattaaa
    15781 gcaaccgaag ctgcggttaa cgaagagttt ggcctggcac cgttcctgcc ggatcagatt
    15841 cacttcgtac acagccagga gttactgtct cgttatccgg atctcgatgc caaagggcgt
    15901 gagcgggcga ttgcgaaaga tcttggcgcg gtattcctcg tcgggattgg cggcaagttg
    15961 agtgatggtc atcgtcacga cgtgcgcgca ccggattatg atgactggag caccccgtca
    16021 gagctgggcc atgcgggcct gaacggcgat attctggtgt ggaacccggt actggaagat
    16081 gcgtttgagc tttcttccat ggggatccgc gtggatgccg acacgctgaa gcatcagctg
    16141 gcgctgaccg gtgatgaaga tcgtctgcag ctggaatggc atcaggcgct gctgcgcggt
    16201 gaaatgccgc agaccatcgg cggcggtatc ggccagtctc gtttgactat gctgctgctg
    16261 caactgccgc atatcggcca ggttcagtgt ggagtatggc cagctgctgt tcgcgagagc
    16321 gtcccttctc tgctgtaata atttatcgcc gccagcgtct gagcaggcgg cttcgcatcc
    16381 cggtatcaaa gcgccagata tgatcgaaaa tgcgcatgat gccgggtttg ccgtgtgccg
    16441 acatcgccac ggcatgaaag cgatgctgat gtacccgctg cagctctttc actttactcg
    16501 tcacgtcgtc aggcaaccgc tgagcgataa aatcagaaat caccaccgca tcggcatcaa
    16561 accattccct gctttgcaag cgttccataa tggcgcgaaa acaactggca agatcggtgc
    16621 cgccacgaaa ctgctggctt aaaaaacgga ttgcttgttc gatgccttgt gggcctgaaa
    16681 gctcataacg gacgatctcg gtggaaaata gcataatata acagcgccgg ttttcagcga
    16741 gagcaatgcg catcaaagcc aggcagaacg ctttcgcaca ctgttcatta aagccgccca
    16801 ttgagccgga agtatccaca cagacaataa acggcccgcg cggctgttca tcgtaatctt
    16861 tatgtaccac cggacgttcg agcacttttt cacgccacga ctcaccgtgc aggcgatagg
    16921 tgagcaactg tttttccacc agccgacggt aaaactcata ctccagttcc gttatcccta
    16981 gtgtcgccag ttctggcggc aggagacgta aaatatcatc gctttgttgc agaccatcaa
    17041 cctgctcagg aaccgtcgcc ggttcgcgca ccatggtgcg gaaggtttcc atctgcgcat
    17101 cgttgcgcgg tattgatttg gcttcccgag aacgccccag ctgctctgct aggcgtttca
    17161 gttccggctg ttcgttaaga aattcaccgt atttcacaat caactgatag tcgccacgtt
    17221 taagctgacc ggcgctcata tcccacagac gaccagctgc ggtattgtta tctgcgagaa
    17281 tcggttcaag ttgtccgctc agcgtcatgc gttcctgaac ttcacttaac agttgttcgc
    17341 gttcttcttc taatagctgt tgatttaacg tcgttgcttg cacgatcaga cttaaacgcc
    17401 aacgctggag aaaaagcgtg tgtaacgctg aagttattgt gctgttagca tcaaccaact
    17461 gccgggcttg ttctgcccat ggggaattca gacgatgcag taagtccagg atctgtggta
    17521 gctgcacaat aaactgcggc gtggagagga gctggctttg ctgatagcac atcacctctt
    17581 cggtgagttc cggcgggact cgggcatctt tcagccgact gcgcagcgcc tcacgccagc
    17641 ggggaacatc atcagtgatt gccgccttca gtcgtgggaa tttttcaaag aagaccgcca
    17701 gctgcggtga ggccagcagt gcgatgatca tctcttcgat caatccctct tcgctgacgg
    17761 ccagcatcac attaagcgta tccagcgtta gcattgttgt gcctggcgaa tctgtgcacc
    17821 gacatcctgt aggctggctt caatgcgacc taaccagtcg ccagggataa acagacattt
    17881 ttgctgttcg ctgaataaag cgtgttgctt acgccagtcg ctttcaagct cttccagttg
    17941 ttgttttatt tcacctggca gaccttcagc cgatgaaccg ggaagtgcca gcgtactgcc
    18001 ttgtaaactt acatcgcgta caacaagatg ttgggtgcta tcaacttcca gattcagttt
    18061 ctgggcaaag ccgataccgt tcagtttgcc gcgaatttca ccacctttac tcagccactg
    18121 ttccagcgcg ctacgctcaa aggagatatg aaccacttcc atatcatgca gttttaacgg
    18181 tttttgcagc agcagagtca gagtggaagc agtaacgtta acaggaagtt gatactgctg
    18241 acgacggctg aaaatgccgc ccagacgaat taacgttaag gctgttttat cgctttgttg
    18301 ctgctggagt tgcaggtgac gttgcacaat cgcgcccagg cgggtcaaca tcccttgctg
    18361 ttgccaggca tgaccggtca tcaatacatc aatttgttgt tgtatcaaat tcaggctttg
    18421 cgcgtcatac cacaggcaat ctttcagcaa aatgagatca accggggcaa cagcactgcg
    18481 accgctaaaa aaggcgctgg cctgcaataa gcggatcgct tttttccagc gacgatccga
    18541 gacataaggc gcatccggta atttatccag ttgctggcgc agcataaaaa tcagctcaaa
    18601 tacatgatcg ggcagcgtaa tttcaccaat ctctttctgc cagcgttcat attcttcatc
    18661 tgtgacctgc aaggcatcag gaaccggatt gtcgttttca tcctgttgac tggtcagcat
    18721 ggagcggaaa ttcgctttat cttgcacttt atctaaccat agacgaatca gcatgcggtc
    18781 atataacgct tccagactgc tgtctgcttc ctgcagctcg ttggaggccg ccaccagtag
    18841 acgcatcggg attttttcta cgtgtgcacc gttgcggaac tggcgctcgt taatggcggt
    18901 gagcaaggta ttaagaattg ccgggcccgc tttccagatc tcatccagaa agacgatttc
    18961 tgcttccggc aggtaaccgc tggttaaacg ttcatagcgc ccttcatctt ttagcgcctg
    19021 aatagaaagg ggaccaaaaa cttcttccgg cgtggagaag cgggtcatca gatattcaaa
    19081 cgcgcgggca ttctgaaagg cgaattttaa gcgccgggcg atcaaacttt tggcaatacc
    19141 tggcgggcca aggaggaaca cactttcacc acttaatgcc gctaataaac acaagcggat
    19201 ggcgtggcta cgttcataaa gccccttttc caacgaactg ctcaggcggg aaattctttc
    19261 cgctaataaa tgagggtgag ccataatgaa gtggcgtcct ttcgtcaaaa gttctgcgta
    19321 aattgcgagt atagacgttt cttgctggtg gctaaaatag tctcaaag
//
LOCUS       DADLSA010000073        19219 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.073, whole genome shotgun sequence.
ACCESSION   DADLSA010000073 DADLSA010000000
VERSION     DADLSA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.073"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            8..2287
                     /locus_tag="JN691_002484"
     CDS             8..2287
                     /locus_tag="JN691_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY7243904.1"
                     /translation="MLVETDGETVFSSRGALATGMENSLQSAVRDQVHSNTRVRFPMV
                     RKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIREAYGPASIFAGSYGWRSN
                     GVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVVGGSEVYQQQTSWPLVLEH
                     SDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKLICIDPMRSETVDFFGDKM
                     EWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYAVFASYLLGESDGIAKNAE
                     WAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGEQKHWMIVTLAAMLGQIGT
                     PGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDKIPVARIVEALENPGGAYQ
                     HNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISECFWTAAAKHADIV
                     LPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAELSERWEKGGYARF
                     TEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPENPDSERFIRFADF
                     CRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGNAEPEQLQVLSAH
                     PAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRVWNSRGQILAGAV
                     ISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLGNGCAGNTALAWL
                     EKYNGPELTLTAFEPPASS"
     gene            complement(2256..2696)
                     /locus_tag="JN691_002485"
     CDS             complement(2256..2696)
                     /locus_tag="JN691_002485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7243905.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2693..3256)
                     /gene="tag"
                     /locus_tag="JN691_002486"
     CDS             complement(2693..3256)
                     /gene="tag"
                     /locus_tag="JN691_002486"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAY7243906.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKAPKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3414..4112
                     /locus_tag="JN691_002487"
     CDS             3414..4112
                     /locus_tag="JN691_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7243907.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4341..5549
                     /locus_tag="JN691_002488"
     CDS             4341..5549
                     /locus_tag="JN691_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7243908.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5873..7564
                     /gene="eptB"
                     /locus_tag="JN691_002489"
     CDS             5873..7564
                     /gene="eptB"
                     /locus_tag="JN691_002489"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAY7243909.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDNYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7656..7732
                     /locus_tag="JN691_002490"
     tRNA            7656..7732
                     /locus_tag="JN691_002490"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7690..7692,aa:Pro,seq:cgg)
     gene            8476..10083
                     /gene="dppA"
                     /locus_tag="JN691_002491"
     CDS             8476..10083
                     /gene="dppA"
                     /locus_tag="JN691_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HAY7243910.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVSPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10391..11410
                     /gene="dppB"
                     /locus_tag="JN691_002492"
     CDS             10391..11410
                     /gene="dppB"
                     /locus_tag="JN691_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAY7243911.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11420..12322
                     /gene="dppC"
                     /locus_tag="JN691_002493"
     CDS             11420..12322
                     /gene="dppC"
                     /locus_tag="JN691_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAY7243912.1"
                     /translation="MSQVTENKVISAPVPMAPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12333..13316
                     /gene="dppD"
                     /locus_tag="JN691_002494"
     CDS             12333..13316
                     /gene="dppD"
                     /locus_tag="JN691_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAY7243913.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13313..14317
                     /gene="dppF"
                     /locus_tag="JN691_002495"
     CDS             13313..14317
                     /gene="dppF"
                     /locus_tag="JN691_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAY7243914.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14347..15618)
                     /locus_tag="JN691_002496"
     CDS             complement(14347..15618)
                     /locus_tag="JN691_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY7243915.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            16094..16201
                     /locus_tag="JN691_002497"
     CDS             16094..16201
                     /locus_tag="JN691_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HAY7243916.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(16288..17967)
                     /gene="bcsG"
                     /locus_tag="JN691_002498"
     CDS             complement(16288..17967)
                     /gene="bcsG"
                     /locus_tag="JN691_002498"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAY7243917.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(17964..18155)
                     /gene="bcsF"
                     /locus_tag="JN691_002499"
     CDS             complement(17964..18155)
                     /gene="bcsF"
                     /locus_tag="JN691_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAY7243918.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(18152..>19219)
                     /gene="bcsE"
                     /locus_tag="JN691_002500"
     CDS             complement(18152..>19219)
                     /gene="bcsE"
                     /locus_tag="JN691_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY7243919.1"
                     /translation="FGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLV
                     QSEEAEIQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSL
                     QQNAQIEPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPL
                     SRCLTMIESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLP
                     AHGKGVLVALRPVPGIRVEQAQTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALN
                     HIFPLPTGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDG
                     RHWRRIPEPMRLLDDAVERSS"
BASE COUNT         4585 a         4818 c         5163 g         4653 t
ORIGIN      
        1 ggggcccatg ctggttgaaa ccgacggcga aaccgtgttt agctcgcgtg gcgcgttagc
       61 cacaggaatg gaaaactcct tgcagagcgc ggttcgcgac caggttcaca gcaatacgcg
      121 ggtacgattt ccaatggtgc gcaaaggctt tctcgcgtca ccggaaaacc cgcaaggcat
      181 tcgtgggcag gatgaatttg ttcgcgtgag ttgggatgag gcgctggatc ttattcacca
      241 acaacataaa cgcattcgtg aggcttatgg tccggcatcg atttttgctg gttcctacgg
      301 ctggcgttca aacggcgtgc tgcataaggc ctcgacatta ttacaacgct atatggcgct
      361 ggcaggcggt tataccgggc atctggggga ttattcgacc ggtgcggcac aggcgatcat
      421 gccgtatgtc gtaggtggca gcgaagttta tcaacagcaa accagttggc cgctggtgct
      481 ggaacatagc gatgtcgtgg tgttgtggag tgctaaccca ctcaatacgc tgaaaattgc
      541 gtggaatgca tccgatgagc aggggctttc ttacttttcc gcactgcgtg acagtgggaa
      601 aaagctgatc tgcattgatc caatgcgatc ggaaaccgtc gatttctttg gcgataaaat
      661 ggagtgggtg gcaccgcaca tgggcaccga tgttgcgctg atgctgggga tcgcccatac
      721 gctggtggaa aatggttggc acgacgaagc gtttctggcg cgttgcacca caggttatgc
      781 cgtcttcgcc tcttatttgc tgggcgagag tgacggaata gcgaaaaacg ccgaatgggc
      841 ggcagagatt tgtggtgttg gcgcagcgaa aatccgcgag ctggcggcta ttttccacca
      901 aaataccacc atgctgatgg caggctgggg aatgcagcgc caacagtttg gtgagcaaaa
      961 acactggatg atcgtcacgc tggcagcaat gttggggcaa atcggcacac ccggcggcgg
     1021 ttttggtctt tcttaccatt ttgccaatgg tggtaacccc acgcggcgtt ctgcggtgct
     1081 ctcttccatg cagggcagct tgccgggtgg ctgcgatgcg gtggataaaa tccctgttgc
     1141 ccgcattgtt gaagcactgg aaaaccctgg tggcgcatat caacacaacg gtatgaaccg
     1201 acatttcccg gatattcgtt ttatctggtg ggcgggcggt gccaacttta ctcatcatca
     1261 ggataccaat cgtctgatcc gtgcctggca aaaaccggag ctggtggtga tctctgaatg
     1321 cttctggacg gcggcggcga aacacgcgga tatcgttctg cctgcgacta cctcttttga
     1381 gcgtaatgat ctcaccatga ccggtgatta cagtaatcag catctggtgc cgatgaagca
     1441 agtggtgccg ccacgctatg aagcgcgtaa tgattttgat gtttttgccg agttgagtga
     1501 acgttgggag aagggcggct atgcgcggtt tacggaagga aaaagtcagc tgcaatggct
     1561 ggaaacgttt tataacgttg cccggcagcg cggggcaagc cagcaggttg aattgccgcc
     1621 atttgctgag ttctggcaag ccaaccagtt aattgagatg ccggaaaacc cggacagcga
     1681 gcggtttatt cgcttcgctg atttttgccg cgatccgctg gcgcatccgt taaaaaccgc
     1741 cagcggcaag attgaaatct tctcacagcg tattgccgat tacggttacc cggattgccc
     1801 tgggcatcca atgtggctgg agccggacga atggcagggc aatgccgaac cagaacagtt
     1861 gcaggtactt tctgcccatc cggcgcaccg cctgcacagc cagctgaatt acagttctct
     1921 gcgcgaattg tacgcggtag caaaccgtga gcctgtcacc attcatcctg acgatgctca
     1981 ggcgcgcggc ataaccgaag gggatatggt gcgggtgtgg aactcccgtg ggcagatcct
     2041 tgccggagcg gtcattagcg agggaattaa acctggcgtg atttgcattc acgaaggggc
     2101 atggccggat ctggatttaa ccgctgacgg tatttgtaaa aacggcgcag tgaacgtgct
     2161 gaccaaagat ctccccagct cgcggctggg gaatggctgt gcgggtaata cggcgctggc
     2221 atggctggaa aaatacaacg gtccggaact gacacttaca gcgtttgaac caccggccag
     2281 ctcataatcc aggtgggtag ttgggtttca tcctgccatg cgcaatcgac aatgtgaaaa
     2341 ccctgcgcac ggtaaaaatc tatcgccggt tgattttttt gataaacctc cagcatcaga
     2401 tggggatagc gctgctgcac atactgcatc agcgccttac caataccgcg tctgacggcc
     2461 ttcggtgcga caaacattgc tgccagaaat cggccttcca taatgctgac aaaaccgaga
     2521 agcttaccgt cctcttccca gacccagttt tgcgcgttgg caagataggc atcccgcacc
     2581 agcggaatgc agtcacgcca gtaattcgct tttataaagg gatgccccca ggttgtactt
     2641 tccagccaca gttcgaggat cgcggggagt tctgaacgtt gcgcttcccg aatcatggtt
     2701 tatttcccgg atagcaacag cagccaacca catgatcatt caccagccca catgcctgca
     2761 taaaggagta acagattgtg gtgccgacaa acttaaaacc acgttttttc ggtgccttag
     2821 atagggcgtc ggaggcggac gtagatgtgg gaatttcgct caacgttgtg gcttgtgtca
     2881 cctgtggctg atgatttaca aacgaccaga caaagtcggg aaacggttcg ccgttctgtt
     2941 ccatttgcag gtacgcccgc gcattaccaa taattgcctg aattttccct cgatggcgga
     3001 taatcccggc gtcctgtacc agtctttcga catcctcttc ctgcattgct gcgaccttca
     3061 ccggatcgaa ctgatgaaag caggcgcgat agttttcgcg ttttttgagg acggtgatcc
     3121 acgataaccc agcctgctgc ccttcaaggc agatcatttc gaacagtttt ttactgtcag
     3181 tttcaggcac gccccactca ttatcatggt aggcaatata aagcgggtcc tgactcaccc
     3241 agccgcaacg ttccatactt tccctcgcta tgtacccgat cgtaaaaatt tcagccgctt
     3301 ttcttgacgc atcggtgaaa aagtcaatat ttaatacagg gctactatgc ccgatatcct
     3361 tcatacaatg acaataatat tgccgaattc ggctcttccc tggagtcgtg ttaatgatca
     3421 taaaaaaaag cggtggtcgc tggcagctaa gcctgctggc gagcgtggta atcagtgcct
     3481 tttttctcaa cacagcttac gcctggcaac aagaatatat cgttgatacg caacccggac
     3541 attccacaga gcgttacacc tgggatagtg atcatcaacc tgattacaac gatattttgt
     3601 cgcaacgtat tcaaagtagc caaagggcgc tgggactgga agtcaatctg gcggaagaaa
     3661 ctcctgtgga tgtgaccagc agtatgagta tgggctggaa ttttccttta tatgaacagg
     3721 ttacaaccgg cccggtcgcg gcattacatt acgatggcac aaccacctcg atgtataacg
     3781 agtttggcga cagtactacc acgctgaccg atccgttatg gcatgccagc gtgagtagct
     3841 taggctggcg tgttgactcc cggcttggcg atctccgacc ctgggcgcaa atcagctata
     3901 accagcaatt tggcgagaat atctggaagg cgcaatcagg cctgagccgg atgacggcga
     3961 caaaccagaa cggcaactgg ctggatgtca ccgtaggcgc tgatatgttg ctcaatcaaa
     4021 atattgccgc ctatgctgcg ctaactcagg cagaaaatac cactaataat agcgactatc
     4081 tgtatacgat gggggttagc gccagatttt aacgtaacag tcacaattga aaccattgaa
     4141 taacaacagt tgtggcgata gtgggtgcta acttatcaaa taataaattt ggtgaataat
     4201 tgtcgcgtca ttcattcctg aactaaggca tttcattccg ttctgatggc atttcatgcc
     4261 gtttttcccc aggcataaaa tgcacttcgt tatggttgtc ggcagagatt tttccttttt
     4321 attactgcag gaatactgcc atgacacctt caaattatca gcgtacccgc tggctgacac
     4381 tcatcggtac tatcattacc cagtttgcgc tggggtcggt ttatacctgg agcctgttta
     4441 atggcgcgct ttctgccaag ctggatgcgc cggtaagcca ggtcgctttc tctttcggct
     4501 tgttaagtct ggggctggca atttcgtctt ctgttgcggg caaattgcag gaacgttttg
     4561 gcgttaaacg cgtcaccgtg gcttccggca ttttgctggg attaggcttc ttcctgaccg
     4621 cgcattccaa caacctgatg atgctgtggt taagcgccgg tgtgctggtc ggtctggcgg
     4681 atggcgcggg ttacctgctg acgctctcta actgcgtgaa gtggttcccg gagcgtaaag
     4741 gtctgatctc cgcgttcgct atcggttctt atggtctggg tagcctgggt ttcaaattta
     4801 tcgacacgca gctgctcgaa acggtgggtc tggaaaaaac ctttgtgatt tggggagcga
     4861 ttgcgctggt gatgattgtt ttcggcgcaa cgttaatgaa agacgcacca aaacaggaag
     4921 tgaaaaccag caatggtgtg gtggagaaag attacacgct ggcagagtcg atgcgtaaac
     4981 cgcagtactg gatgttagcg gtaatgttcc tgactgcctg catgagcggc ctgtacgtga
     5041 ttggggtagc gaaagatatc gcccaaagtc tggcacacct tgatgtggtt tccgcagcca
     5101 atgcagtaac ggttatttcc atcgctaacc tttcaggtcg tctggtgctg ggcattctgt
     5161 ctgacaaaat tgcccgtatc cgtgttatca ccattggtca ggtgatttcg ctggtgggta
     5221 tggcggccct gctgtttgca ccattaaatg cagtgacgtt ctttgcagcg attgcctgcg
     5281 tggcatttaa ctttggcggc actattaccg tctttccatc actggtcagt gagttcttcg
     5341 gcctcaataa cctggcgaaa aactacggtg tgatttacct cggtttcggt atcggtagca
     5401 tttttggttc gattatcgcc tcactgtttg gcggtttcta tgtgaccttc tacgtgattt
     5461 tcgccctgct gattctgtca ttggcgcttt ctacgacgat tcgtcagcca gagcagaaaa
     5521 tgttgcgtga ggcgcatggc tccctttaat gctcagccgc gagctactgg gcttcagctt
     5581 ccgtgtgcat caaacgcatc aacgaaatgt ataaacaatg actcgtaaaa tttgggccag
     5641 ttaactggcc ctttttactg ctttttgtaa cgctaaagtc tcttttcaaa cttgcatttt
     5701 tgtaaatttg tgcttcatgc acactctttc cccacacttt ttcgctttgc tgtggtctac
     5761 ttattcgcgc gtgtagattt tacttatctg actacctccg cacttttccc ctgccgggcc
     5821 tgaaaagccg ctaagcaggg tgttatcacc tgtttgtcca gggtttgttt gcatgagata
     5881 catcaaatcg attacacagc agaagctgag ctttttgctt gcaatctata ttggcctttt
     5941 tatgaatggc gcggtttttt atcgccgctt cggcagctat gcgcacgatt ttaccgtctg
     6001 gaaaggcgtt tctgctgttg ttgaactggc cgccaccgta ctggtgacct tctttttact
     6061 acgtcttcta tcgctgtttg gccgccgcag ctggcgtatt ctggcgtcgc tggtggtgct
     6121 cttttccgca ggtgccagct attacatgac cttccttaat gtggtcattg gttatggcat
     6181 catcgcttcc gtcatgacca ccgatatcga cctgtcaaaa gaagttgttg gtctgaactt
     6241 tattctctgg ttaatcgccg ttagtgcatt gcctcttatc cttatctgga ataaccgctg
     6301 tcgctacacc ttgctccgac aactgcgaac cccagggcag cgtattcgca gcctggcggt
     6361 cgtcgtactg gcgggtatta tggtttgggc accgattcgt ttgctggata tccagcagaa
     6421 gaaagtggag agggcgaccg gcgttgattt gccgagttat ggcggtgtcg tagcgaactc
     6481 ttatctgcca tcaaactggc tttctgcgtt ggggctgtat gcctgggcgc gggtcgatga
     6541 atcttccgat aataattcat tgcttaatcc ggcgaagaag tttacatacc aggctccaca
     6601 aaacgttgat gatacttatg tcgtgtttat catcggtgaa accacgcgtt gggaccatat
     6661 gggtattttc ggctatgagc gtaataccac gccgaaactg gcccaggaga aaaatctggc
     6721 ggcgttccgt ggttactcct gtgataccgc aaccaaactc tcactgcgtt gcatgtttgt
     6781 acgtcagggg ggcgcggaag ataatccgca gcgcacatta aaagaacaga acattttcgc
     6841 ggttctgaag cagttaggat tcagttctga cctctacgct atgcagagcg aaatgtggtt
     6901 ctacagcaac acgatggcgg acaacattgc ttatcgtgag cagattggtg cggagccacg
     6961 taatcgtggc aagccggtag atgatatgtt gctggtagat gaaatgcagc aatcgctagg
     7021 gcgcaacccg gatggtaagc atctgatcat tctgcatacc aaaggttcgc attttaacta
     7081 cacccagcgt tatccgcgta gcttcgcgca gtggaagccg gaatgtattg gtgttgatag
     7141 cggctgtacc aaagcgcaga tgatcaactc ctatgacaac tcggtgacct atgtggatca
     7201 ctttatctcc agcgtgattg atcaggttcg cgataagaaa gcgattgtgt tctacgcagc
     7261 tgaccacggt gagtcaatta atgaacgcga gcacctgcac ggcacgccgc gtgaactggc
     7321 accgccggag cagttccgcg taccgatgat ggtctggatg tcagataact atctggaaaa
     7381 tccggccaat gcgcaggcgt ttgcgcagct gaaaaaagaa gccgacatga aagtgccacg
     7441 ccgtcacgta gagctgtacg acaccatcat gggttgtctt ggctatactt caccggatgg
     7501 tggaattaac gaaaacaaca actggtgtca catcccgcag acaaaagtgg cagcggctaa
     7561 ctaagcggcc tgctgacttt ctcgccgatc aaaaggcatt ttgctattaa gggattgacg
     7621 agggcgtatc tgcgcagtaa gatgcgcccc gcattcggtg attggcgcag cctggtagcg
     7681 cacttcgttc gggacgaagg ggtcggaggt tcgaatcctc tatcaccgac caaattcgaa
     7741 aagcctgctc aacgagcagg tttttttgca tttgtagctc atgaggatga gaacctccgg
     7801 gggcagggag gctcgactcg acgtaggcct gataagacgc gccagcgtcg catcaggcaa
     7861 cggcaccgca cttattgtcg gatgcggcgc gaccgcctta ttcgaccaac acaccacaca
     7921 ttttcctgcc aactttgtgc cccatatctc actatccatt aacggatttg tgacagaaac
     7981 cattgtcttt ttttatattt gcttaaatct tttttgcgtt acttatggct ggcgctggca
     8041 ttttccgctg tgcgttttag cgttcatggt attaatcgct cagatatcca ccttttcctc
     8101 agattttttt cattaaatgc ataaaactta atcaccaaat gttgctaaat attaagctga
     8161 ttgttctggt tatgttggag tagcacaaat ttctctgctg caaatagctg tttgaagttt
     8221 ttttaatctt tgtttgtgga ttctcaccgt tggtgtaaat cccggttggc tgtattgacg
     8281 ttttcacatt ctgttgacag attgtaggtc acgaggggca ttttatggag gatccgcact
     8341 gttacactga tgttaattag tacggcatcc ccacctcata acgttgaccc gaccgggcaa
     8401 aaaacaaaaa aggtcaggca gcgacaaccc attgcaaagg gttaaaacaa caaacatcac
     8461 aattggagca gaataatgcg tatttccttg aaaaagtcag ggatgctgaa gcttggtctc
     8521 agcctggtgg ctatgaccgt cgcagcaagt gttcaggcta aaactctggt ttattgctca
     8581 gaaggatctc cggaagggtt taacccgcag ctgtttacct ccggcaccac ctatgacgcc
     8641 tcttccgtcc cgctttataa ccgcctggtt gaatttaaaa tcggcaccac cgaagtgagc
     8701 ccgggcctcg ctgaaaagtg ggaagtcagc gaagacggta aaacctatac cttccatctg
     8761 cgtaaaggtg tgaagtggca cgacaataaa gaattcaaac cgacgcgcga aatgaacgcc
     8821 gatgacgtgg tgttctcgtt cgatcgtcag aaaaacgcgc aaaacccgta ccataaagtt
     8881 tctggcggca gctacgaata cttcgaaggt atgggcttgc cggagctgat cagcgaagtg
     8941 aaaaaggtgg acgataacac cgttcagttt gtgctgactc gaccggaagc gccgttcctc
     9001 gctgacctgg caatggactt cgcctctatt ctgtcaaaag aatatgctga cgcgatgatg
     9061 aaagccggta caccggaaaa actggacctc aacccaatcg gaaccggtcc gttccagtta
     9121 cagcagtacc aaaaagattc ccgtatccgc tacaaagcgt ttgatggcta ctggggcacc
     9181 aaaccgcaga tcgatacgct ggtcttctct attacccctg atgcttccgt gcgttacgcg
     9241 aaattgcaga agaatgaatg ccaggtgatg ccgtacccga acccggcaga catcgctcgc
     9301 atgaagcagg ataaatccat caacctgatg gaaatgccgg ggctgaacgt cggctacctc
     9361 tcgtataacg tgcagaaaaa accgctggat gacgtgaaag ttcgccaggc actgacctac
     9421 gcggtgaaca aagacgcgat catcaaagcg gtttatcagg gcgcgggtgt atcagcgaaa
     9481 aacctgatcc cgccaaccat gtggggctat aacgacgacg ttcaggatta cacctacgat
     9541 cctgaaaaag cgaaagcctt gctgaaagaa gcgggtctgg aaaaaggttt ctccatcgac
     9601 ctgtgggcga tgccggtaca acgtccgtat aacccgaacg ctcgccgcat ggcggagatg
     9661 attcaggcag actgggcgaa agtgggcgtg caggccaaaa tcgtcaccta cgaatggggt
     9721 gagtacctca agcgtgcgaa agatggcgaa catcagacgg taatgatggg ctggactggc
     9781 gataacgggg acccggataa cttcttcgcc accctgttca gctgcgccgc ctctgaacaa
     9841 ggttctaact actcaaaatg gtgctacaaa ccgtttgaag atctgattca accggcgcgt
     9901 gctaccgacg accataacaa acgcgttgaa ctgtacaagc aagcgcaggt ggtgatgcac
     9961 gatcaggctc cggcactgat catcgctcac tccaccgtgt ttgaaccggt acgcaaagaa
    10021 gtcaaaggct atgtggttga tccattaggc aaacatcact tcgaaaacgt ctctatcgaa
    10081 taattaaaag ccatacaaga ctgatggcaa aggcaaaact gcctgatgtg ctccgcttat
    10141 caggcctacg aaaattctgc aatgtattga atttgcacga ttttgtaggc cggataaggc
    10201 gttaacgccg catccggcat aaacaaagcg cactttgtca acaatctgta tacccggtgg
    10261 cgctgtgcct ctgcgtgcag cgccatccgg cagcaatact tcattctctg tccgacaagg
    10321 gcgggaattg atttgtgagc aatacagaca cgcagttcca ggctgcgggt cactatagag
    10381 aatccgggtt atgttgcagt ttattctccg acgtttggga ctcgtcatcc ccacgtttat
    10441 cggtattacc cttctcacat ttgcctttgt ccacatgatc ccgggcgatc cggtgatgat
    10501 catggcaggc gaacgtggga tctcccctga gcgtcacgtg cagctgctgg ctgaactcgg
    10561 cttagataaa ccgatgtggc agcagtatct ccattacatt tggggcgtaa tgcacggcga
    10621 tctaggcatt tcaatgaaaa gccgaattcc agtatgggaa gagttcgtgc cgcgctttca
    10681 ggccacgctg gaacttggcg tctgcgcgat gatttttgct acggcagtcg gtattccggt
    10741 tggtgtgctg gctgcggtta aacgcggttc cattttcgat cacactgcag ttggcttggc
    10801 gctgaccggc tactcgatgc cgatcttctg gtggggcatg atgctgatca tgctggtttc
    10861 ggtgcactgg aacctgacgc ccgtctccgg tcgcgtgagc gatatggtgt tcctcgatga
    10921 ctccaatccg ttaaccggtt ttatgctaat cgacaccgcc atctggggtg aagacggcaa
    10981 ctttatcgat gccgtcgccc atatgatctt gcccgccatt gtgctgggta ctattccgct
    11041 ggcggtcatt gtgcgtatga cacgctcctc gatgctggaa gtgctgggcg aggattacat
    11101 ccgcaccgcg cgcgccaaag ggctaacccg catgcgggtg attatcgtcc atgcgctgcg
    11161 taacgcgatg ctgccggtag tgaccgttat cggcctccag gtgggaacat tgctggcggg
    11221 ggcgattctg accgaaacca tcttctcgtg gcccggtctg gggcgctggt tgattgacgc
    11281 actgcaacgc cgcgactatc cggtagtgca gggcggcgta ttgctggtgg cgacgatgat
    11341 tatcctcgtc aacttgctgg tcgatctgct gtacggcgtg gtgaacccgc gtattcgtca
    11401 taagaagtaa ggggacatca tgtcacaggt tactgaaaat aaagtgatta gcgcaccggt
    11461 gccgatggcc ccgttacagg agttctggca ctattttaaa cgcaacaaag gcgcggtcgt
    11521 cgggctggtt tacgtcgcca tcgtgctgtt catcgcgatc tttgccaact ggattgcgcc
    11581 ttataacccg gcggatcagt tccgcgatgc gctgctcgcc ccgccagcct ggcaggaagg
    11641 cggcagtatg gcacacttgc tgggcaccga tgacgtcggt cgtgatgtgc tgtcgcggct
    11701 gatgtacggc gcgcgtttat cgctgctggt tggctgtctg gtggtggtgt tgtcgctgat
    11761 tatgggcgtt attctcggcc tgatcgccgg ttacttcggc ggcctggttg ataacattat
    11821 catgcgcgtg gtcgatatca tgctggcgct gccaagtctg ctgttggcac tggtgctggt
    11881 ggcgattttc ggcccgtcga ttggtaacgc cgcgctggcg ctgaccttcg ttgccttgcc
    11941 gcactatgtg cgcttaaccc gcgccgccgt gctggtggag gtgaatcgcg attacgtcac
    12001 cgcctctcgc gtggcaggtg ccggggcgat gcgccagatg tttattaaca tcttcccgaa
    12061 ctgccttgcg ccgctgattg ttcaggcgtc gctcggtttc tctaacgcca ttctcgatat
    12121 ggctgctctt ggtttcctcg gcatgggggc acagccgcca acgcctgagt ggggcaccat
    12181 gctctccgac gtgttgcagt tcgcgcaaag cgcctggtgg gtcgtgacct tccctggtct
    12241 ggcgatcctg ctgacggtgc tggcatttaa cctgatgggt gacggtctgc gtgacgcgct
    12301 cgatcccaaa ctgaagcagt aagaggttcg agatggcgtt attaaatgta gataaattat
    12361 cggtgcattt cggcgacgaa agcgcaccgt tccgcgccgt agaccgcatc agctacagcg
    12421 taaaacaggg cgaagtggtc gggattgtgg gtgagtccgg ctccggtaag tcggtcagtt
    12481 cactggcgat tatggggctg attgattatc cgggccgcgt aatggcagaa aaactggagt
    12541 ttaacggcca ggatttgcag cgtatctcag aaaaagagcg ccgcaacctg gtgggtgccg
    12601 aagtggcgat gatcttccag gacccgatga ccagccttaa cccgtgctac accgtgggtt
    12661 tccagattat ggaagcgatt aaggtgcatc agggcggcaa caaaagtacc cgccgtcagc
    12721 gggcgattga cctgctgaat caggtcggta ttcccgatcc ggcttcgcgt ctggatgttt
    12781 acccgcatca gctttccggc ggcatgagcc agcgcgtgat gatcgccatg gcgattgcct
    12841 gtcggccaaa actgctgatt gccgatgaac cgaccaccgc gctggacgtg accattcagg
    12901 cgcaaatcat cgaactactg ctggagctac agcagaaaga gaacatggcg ctggtgttaa
    12961 ttacccatga cctggcgctg gtggcggaag ctgcgcataa aatcattgtg atgtatgcag
    13021 gtcaggtagt agaaacgggc gatgcacacg ccatcttcca tgcgccgcgt cacccgtata
    13081 ctcaggcatt gctgcgtgcg ctgccagaat ttgctcagga caaagaacgt ctggcgtcgt
    13141 tgccaggtgt tgttcccggc aagtatgacc gcccgaacgg ctgcctgctt aacccgcgct
    13201 gcccctatgc cactgacaga tgtcgcgctg aagaaccggc gctgaatatg ctcgctgatg
    13261 ggcgtcagtc caaatgccat tacccacttg atgatgccgg gaggcccaca ctatgagtac
    13321 gcaagaggcc acctcgcaac aaccgctgtt gcaggctatc gacctgaaaa aacattatcc
    13381 ggtgaagaaa ggcatgttcg tgccggaacg tctggtgaag gcgctggacg gcgtttcgtt
    13441 taaccttgaa cgtggcaaaa cgctggcagt agtgggcgaa tctggctgcg gtaaatcgac
    13501 cctcggtcgg ttgctgacga tgatcgaaac gcccaccggc ggcgagctgt attatcaggg
    13561 gcaggatctg ctcaagcacg atccgcaggc gcagaagctg cgtcggcaga aaatccagat
    13621 cgtcttccag aatccctacg gttcgctgaa tccgcgtaag aaagttgggc aaattctcga
    13681 agagccgctg cttattaaca ccagcttaag caaagaacag cgtcgggaaa aagccctgtc
    13741 gatgatggca aaagtcggcc tgaaaaccga gcactatgac cgctatccgc acatgttttc
    13801 cggcggacaa cgtcagcgta tcgctattgc ccgtggtctg atgctcgacc cggatgtggt
    13861 gattgccgat gagccggttt ctgcgctgga cgtctcagtg cgtgcgcagg tgctgaatct
    13921 gatgatggat ttgcagcagg agttggggct ttcttatgtc tttatctccc acgacctgtc
    13981 ggttgtggag cacattgctg atgaagtgat ggtgatgtac ctaggccgct gcgtggagaa
    14041 aggaacgaag gaccaaatct tcaataaccc gcgccatccg tacacccagg cgctgctctc
    14101 cgcgacgcca cgcctgaacc cggacgatcg tcgtgaacgc atcaaactca ccggtgaact
    14161 gccaagcccg ctgaatcctc cgccgggttg cgccttcaac gcccgctgtc gtcggcgctt
    14221 cggcccctgc acccagttgc agccgcagct aaaagactac ggcggtcaac tggtagcttg
    14281 ttttgctgtt gatcaggatg aaaatccgca gcgttaactc acaaaaccgg ggccatgcgc
    14341 tccggtttac tcaatcagct tcaacagtgg cgacaagcat agcaaaatgc cgtaaagcag
    14401 aatcagccag gctttaaatc cgcgaagttt ttccagttgt gcgactttat agatcaggaa
    14461 aaacggaatg aggcacgaca caatgccata taacgggctt cccaactgaa agaacaccag
    14521 cactgataca cgaaacgaaa cccaaatcgt caacgtaatg acgataaaag cgcagatcgc
    14581 cagagtcagc atgcgtgagt taattttctt ggtatcaata attcggctta acagattgag
    14641 aataatgcct ttaatagcct cgtggaaacc gagataaatg ccaaagaatg cggtcagtac
    14701 ggcaaagata ttcagcaccg tagaggtgat atgaatgata tgtccaggga tcacctgcgc
    14761 ggccagtgcc agtgccgaga tattttgttc aaaggcagaa acggcttctt cgtggctaat
    14821 tgagaaggta aacgaaaagg caaaaaacag gatcaccgcg atgagcgtga tataactaat
    14881 tcggtgggta cgcagcgcga gccgggttgc cagtacttta tctgcttccc gtttacgata
    14941 ggcaatattc attgggttaa gtacctgaat aaataccgca gaaaagaagc aaaatggaat
    15001 ggtaagcagg acatcgcgga aaaagacgga ggcttgcggg aaggcggtta tattggcgaa
    15061 attccagtgc gggatcatcg caaaaccgaa cacgacaata atccctactt tgaccaccac
    15121 cattggccca gaaatcttaa acagtaatcg ttcaccacca gacgcaatcg ctatcagtac
    15181 ggcgaaaata gcgactttat aaagtagaga ttgtgaaaga tcggcatcgg ttaaaccgaa
    15241 ggttttcagg tacgaggcgc tgtcgaaaac cacggagaga gagtagataa aaatcccgtg
    15301 gataatcatc aaaaagtaga taaccccgag gaaaattccc cagttcttcc ccaggtaatg
    15361 actgataata tcggtgtagt cattacagga atcgctttca gaaagggttt ttaaataaat
    15421 gtcctgcact acccaggtgg caggataagc aatgatcgct gcggtaataa atacccaaat
    15481 tcccttcaag ccaatttgta ctggcatcag cacggttccg gcaccaatcg ccatgcctat
    15541 gcataataaa acccagccga agtcgtagcg tgtaaacggc aatttctgat tgtgtttcga
    15601 tagtgtgttg tgctgcataa agtcctgtcc agaaagaagt cggcctcgca aaaatgaata
    15661 aacccagttt ctcttgcgca acaggtggag aagtcgaata cgcagtatgt aaaaaatgag
    15721 gcaacagatt cactggggag gtcgcgagga ggcgcaattc tacaaaattg cgcatttcac
    15781 taccagtctg gagtcagccc ggatatgaac tgcaactatt gcaaataaag aaattaagct
    15841 ggaaggcatc gcgcaatgag gaagattgcc gggtgctgac agtaaaaagc aatcgtagaa
    15901 tcagcggcaa gccggaaagg ttccggtgag gcgcaatgtt gcgggggctt tatccctggt
    15961 ggcattggtt gctggagaga gaaaaccccc gcacgttgca ggtatgcacc tgacaacatc
    16021 acgggggcta atcttgaacc caaacaactc aagaatagcc gcgaaacgtt ggcattacaa
    16081 cacaggcggc tatatgacgc tcgcagaact gggcatggcc ttctggcatg atttagcggc
    16141 tccggtcatt gctggcattc ttgccagtat gatcgtgaac tggctgaata agcggaagta
    16201 acgtgtcatg cgggcgtcag gctgccgtaa tggcaatttg cgcccggatc aggccgcaga
    16261 ggttaaactc tgcggccttt ttcgtgctta ctgcgggtaa ggcacccagt cgccgccgtt
    16321 caggcgaacg tacggtttat cttggtattg aataactact gcatttgagt tctcggagac
    16381 cggtgctgtt tgtggcaacc cactggtgag ttttttccag tcaacattgt cttcggtgaa
    16441 aatcttgcca tcaagaacgc gaaccaccag atcggagata gccaggaagc tgctcggttg
    16501 gtcaatgaca atcggtgccc cctgatgtgg tgccttcatg ccgaagaatt tcaccccaac
    16561 ggggacgtct gtgatagacg ggctagggat atcacgtagg ccagatacct gcattctgtc
    16621 gcccttcagc gcgccgccgt gttccggcac cacgaccacc atcactttac gacccgattt
    16681 ctccagttca gtaaagaagg cgtccagttc atcaaagaat ttctgcgccc gcgctttgta
    16741 atccgctgtt ttgctgaccc ccggataatg gttgccgtca tgcagtggaa gcgtgttgta
    16801 gaacgtggca ctacggctgt ttttatcttt ttcggtaacg tccagccagc ggttaagcac
    16861 ggcggtatcg tcataaaccg gcgaaccatc aaagcccagc aaaataaccg gcagatttgt
    16921 ttgatccatc aattcagtct gcatgccgcc attttcgcga acttctttca aaaaaccgcc
    16981 gaactggccg ttatgtccca tcatcaggtg ctgggtaaag cccagtttcg aaaggttatc
    17041 aaacagatag cagtcgttat ttgccggttg atacagatta gtgtgcgaag tctgcccgca
    17101 gctggcgcgc agtaaacgga tcgccgccgg gccactgtag gaggtggcgg agttaaagtt
    17161 cttgaactca atatcgaaat gcgaccacag tggatgcgac atcaacccgg cggcttctat
    17221 atccgaccag gaaagcgaac agatgttaat caccagtagt tcaaatggct gagcatcagc
    17281 gggcagcgaa gacgggaagg tcgatttacg tttcgcctcc gcgttataga aattattcag
    17341 ccaggcgtta aggttcgccg ttgttggcgg tgcagtttgt gcgggcatat cacccactac
    17401 cggtgcgcca cccgtcgccg caacggttgc cgctgcgtta ccacccgtcg ttgttacagt
    17461 ggtcgtcggt tgtccggctg gccacaagga gaaacttggt cccgccaggg taagtacgtt
    17521 cagccatagc agtatggcaa ccacaaaaac ggtaatgcga atccattgtg acaggaataa
    17581 ccaggccact aataaaacaa aaatggcccc aatcatctgc cagttaataa agcgtgtgac
    17641 aaggtcgatt aaataatcgg tactgaaccc cgccacctgc gaaccctggc tcattatgct
    17701 ttccgggcca ggcaaccagg tgtcatgcca gaacaaagca aagccgatcg gcagggcaat
    17761 ccagtggcgc aagcgatgca ggctgtaacg cggaatgggc atcagcagaa acgcggcaaa
    17821 caccaaattg aggagcgggt ggaagttaag atatcccgcc cacaacaggc caaacttaac
    17881 cagaaaatag aagttccagc cggaaaggcc gcgccagtat tgccagaggg aagaaggcat
    17941 ggcggtattt tgcgtaaatt gagtcatttt ttggttgccc tggctttttc cgtgcggcgt
    18001 aacgtcccgg ccggtttaac ataacgaggt ttagcaaaga acaaacgtaa ggtgtcgcga
    18061 atgcgtcgca aagagtgccg cgccagatag cccagcggga aaaatatcag tgcgcaaacg
    18121 acaataattt caatgatatc gctgatggtc atcatgatga gcgttccaca gcatcatcta
    18181 acagtcgcat gggttctggt attcgtcgcc agtggcgacc atcgtgctcg gcattgatga
    18241 ccggtttaga actttgcgtt aaaggcagcg gcattcccca ttgttctggg gcaagcaggc
    18301 gcatctgcac cagctcggca ctgatttgat catcttcaaa ccagaccata cggtttgaga
    18361 aaatgtcgcc agtaggcaat gggaaaatat gattcaacgc ggtatccaga tcgttaatcc
    18421 gacagaatga gagaaacagc accagccgat taccgccaat ggtcatgata tcgccggtac
    18481 ggttagggcg acatagcgtc tgggcttgct caacgcggat acccggtacc ggacgtaggg
    18541 caaccagaac gcctttaccg tgggcaggca agagagggtt attcatcatg ttgttgacgg
    18601 cattacagaa cacatcccac ttctggaaac cacgcagttt gagcggctgg gtcattgaca
    18661 gcaaggtagt gatatcttcc ggtacatagc gactaaactt ctgcccttgc acgctttcga
    18721 tcatcgtcag acaacgggag agtggcgcat tccacggaat aaccatattt gcaccgcagg
    18781 ccaataacaa acgttcatcg gtggcgcgca ggctagcggt attttcccgc acgaggattt
    18841 tcatcgcact accgcgctgg cgacgcaggg tatgaatgct gcgggccagt ggctcgattt
    18901 gcgcattttg ctgtaaagaa aagaccaccg tcgccgcctg agcggtacgg gcttcattga
    18961 acaggacttc gttattgttg aacagttgcc agtgttccga tagcggcggc gcaccttcca
    19021 gtacagcaac attactcaga atgcgttttt cgtcgctgcg tggttggatc tccgcctctt
    19081 cgctttgaac taatgtccag ataccatttt gttgctgaac gctaagctgc tgacgggcgc
    19141 tgaccccttt ttcgttgcac cagaaggcaa tatccagcaa atgttggtca ccctgaaaac
    19201 gcaaactggc aagaccaaa
//
LOCUS       DADLSA010000074        18938 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.074, whole genome shotgun sequence.
ACCESSION   DADLSA010000074 DADLSA010000000
VERSION     DADLSA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18938
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.074"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            426..1007
                     /locus_tag="JN691_002501"
     CDS             426..1007
                     /locus_tag="JN691_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY7243920.1"
                     /translation="MKQQEEHNNKIVLLEKQQAQLKSQLETIQKQQTGIINSTKNLTH
                     VIKSVKDQQNTFIFTEFNPAKTKYFILNNGSVALAGRVLSIDATENGSVIHISLVNLL
                     STPISNIGFNATWGGEKPVDAKEFARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVS
                     PNNLGYLKLAINMENIQFDNLPSAENRQKRSKK"
     gene            1051..5713
                     /locus_tag="JN691_002502"
                     /pseudo
     CDS             1051..5713
                     /locus_tag="JN691_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            6081..7736
                     /gene="lldP"
                     /locus_tag="JN691_002503"
     CDS             6081..7736
                     /gene="lldP"
                     /locus_tag="JN691_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAY7243921.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7736..8512
                     /gene="lldR"
                     /locus_tag="JN691_002504"
     CDS             7736..8512
                     /gene="lldR"
                     /locus_tag="JN691_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAY7243922.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8509..9699
                     /gene="lldD"
                     /locus_tag="JN691_002505"
     CDS             8509..9699
                     /gene="lldD"
                     /locus_tag="JN691_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAY7243923.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9895..10368
                     /gene="trmL"
                     /locus_tag="JN691_002506"
     CDS             9895..10368
                     /gene="trmL"
                     /locus_tag="JN691_002506"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAY7243924.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10421..11242)
                     /gene="cysE"
                     /locus_tag="JN691_002507"
     CDS             complement(10421..11242)
                     /gene="cysE"
                     /locus_tag="JN691_002507"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAY7243925.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAISIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11322..12341)
                     /gene="gpsA"
                     /locus_tag="JN691_002508"
     CDS             complement(11322..12341)
                     /gene="gpsA"
                     /locus_tag="JN691_002508"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAY7243926.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12341..12808)
                     /gene="secB"
                     /locus_tag="JN691_002509"
     CDS             complement(12341..12808)
                     /gene="secB"
                     /locus_tag="JN691_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAY7243927.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12871..13122)
                     /gene="grxC"
                     /locus_tag="JN691_002510"
     CDS             complement(12871..13122)
                     /gene="grxC"
                     /locus_tag="JN691_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAY7243928.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13264..13695)
                     /locus_tag="JN691_002511"
     CDS             complement(13264..13695)
                     /locus_tag="JN691_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY7243929.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13940..15484
                     /gene="gpmM"
                     /locus_tag="JN691_002512"
                     /gene_synonym="pgmI"
     CDS             13940..15484
                     /gene="gpmM"
                     /locus_tag="JN691_002512"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAY7243930.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15494..16777
                     /gene="envC"
                     /locus_tag="JN691_002513"
     CDS             15494..16777
                     /gene="envC"
                     /locus_tag="JN691_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAY7243931.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16781..17740
                     /locus_tag="JN691_002514"
     CDS             16781..17740
                     /locus_tag="JN691_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAY7243932.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17727..18761)
                     /gene="waaH"
                     /locus_tag="JN691_002515"
     CDS             complement(17727..18761)
                     /gene="waaH"
                     /locus_tag="JN691_002515"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HAY7243933.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPIDRLRSTGVLTGPDWLRMGLFSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
BASE COUNT         4796 a         4867 c         4874 g         4401 t
ORIGIN      
        1 tacgtctctt tttttcggat ttaattgtga aattttaatc atcatattca caaaatcaat
       61 tatctgtaaa atttttccca gacatggttt ctttcatatt tgacatttat taataatcat
      121 cattagaatc cacgaaaaat aatatattca tacaaacaca gacatctttt gaaacgaatg
      181 atacattttt atgatgaatg cttaaaacaa tatttaatcg acacacatca ctgtcattaa
      241 tgtaaagcaa catagctcac caataataca atggacaact ctaccggcat tgatcgctgg
      301 tcggaggaaa taaggatttt tacatgaact tgaagaagat ttttttcagt gctgtcactg
      361 tttcagttct ctgcgcatta acgggatgtg actacattga agaaggcaag ccagaaagta
      421 gtttactgaa acaacaagaa gaacataaca ataaaattgt gctacttgaa aaacaacaag
      481 cacagctaaa gagtcaactc gaaactattc aaaaacaaca aactggcata ataaacagta
      541 caaagaattt aactcacgta attaaatccg ttaaagacca acaaaatact tttattttta
      601 ccgagtttaa cccagcaaaa acgaagtatt tcatcctgaa caacggttcg gtggctttag
      661 cgggtcgagt gttatccatt gacgccacag agaatggtag tgttattcat atctcactgg
      721 tcaacttatt aagtacacct atctcaaaca ttggttttaa tgcgacatgg ggtggtgaaa
      781 aacctgtcga tgccaaagag tttgcccggt ggcaacaatt acttttcaac acatcaatga
      841 aatccacatt gaaattatta ccaggtcaat ggcaagacat taatttgacc ctgaagggtg
      901 tatcgcccaa taacctagga tatctgaagt tagccatcaa catggaaaat attcagttcg
      961 acaatcttcc ctctgctgaa aatcggcaga aaagaagcaa aaaataaatg cagttagtac
     1021 gcttataaca aggaatagcg agcatcatta atgaacaaaa tatttaaagt tatctggaac
     1081 cctgcgacag ggaattatac tgttaccagc gaaacggcaa aaagccgtgg caagaaatct
     1141 gggcgcagta agctgttaat ttctgcgctg gttgcgggtg gaatgttgtc gtcgtttggg
     1201 gcattggcga atgccgggaa tgacaacggt cagggtgttg attacggtag tggatcagct
     1261 ggcgacggct gggttgctat aggcaaaggg gcgaaagcaa atacttttat gaacaccagt
     1321 ggttccagta ctgctgtggg ttatgacgct atagctgaag gccaatatag ctctgccatc
     1381 gggtcaaaaa cccatgcgat tggtggtgca tcaatggcct ttggggttag tgcaatatca
     1441 gaaggcgata gaagtatagc actgggtgcc tcttcgtatt cattgggcca atactcaatg
     1501 gccctcggcc gttattcaaa agcattgggt aaattgtcta ttgctatggg ggactcttcc
     1561 aaagcggaag gagcaaacgc cattgccctg gggcgtagca gtgtagctag cggtactgac
     1621 agcctcgcat ttggcagaca atcacttgcc agcgcagcga acgctattgc gataggtgct
     1681 gagaccgaag ccgctgaaaa tgcaactgct attggcaata atgcgaaggc aaaagggact
     1741 aatagcatgg caatggggtt cggaagcctt gccgataaag tcaatactat cgcattagga
     1801 aatggcagcc aggctctggc agataatgca atcgccatag gccagggcaa caaagctgat
     1861 ggcgtggatg ccatcgctct gggtaatggt agccagtcga gaggcttaaa caccattgcc
     1921 ttaggcacag ccagtaatgc aactggtgat aagagtcttg cgcttggtag taatagcagt
     1981 gccaacggta ttaactctgt cgcgctgggc gcagattcca ttgcggattt agacaatacc
     2041 gtctctgtcg gcaatagttc attaaaacgc aagatcgtta atgtgaaaaa tggcgcgatc
     2101 aagtctgaca gttacgatgc cattaatggt tcacagcttt atgccattag cgactcggta
     2161 gcaaaaaggc ttggaggagg ggctgcagta gatgttgatg acggtactgt tacagcacca
     2221 acctacaatt taaaaaatgg tagcaaaaat aacgtagggg ctgcgctcgc tgtacttgat
     2281 gaaaacaccc tgcaatggga ccaaaccaaa ggcaaataca gcgctgctca tggtactagt
     2341 agcccaactg ccagcgtaat caccgatgtt gcggatggca cgatttcagc ctccagtaag
     2401 gatgcggtta acggttccca actgaaagct accaatgacg atgtcgaagc caacaccacc
     2461 aatatcacca acctgacgga ttccgttggt gaccttcagg ctgatgccct gctctggaac
     2521 gaaactaaaa aggcattcag tgcagctcac gaccaggata ccaccagcaa aatcaccaac
     2581 gttaaagatg ccgacctgac ggctgacagc actgatgctg ttaacggctc tcagctgaaa
     2641 accaccaacg atgctgtggc gacgaatacc accaatatcg ccaataacac ttccaatatt
     2701 gccactaaca ccaccaacat ctctaacctg actgagacgg tgactaatct tggtgaggat
     2761 gcgctgaaat gggataagga caatggtgta ttcacggcag ctcatggcac cgagaccacc
     2821 agcaaaataa ccaacgttaa agatggcgac ctgacgactg gcagcaccga tgccgttaac
     2881 ggctctcagc tgaaaaccac caacgatgcc gtggcgacga ataccaccaa tatcgccact
     2941 aacaccacca acatctctaa tctgactgag acggtgacta atcttggtga ggatgcgctg
     3001 aaatgggata aggacaatgg tgtcttcact gcagctcatg gcaacaatac cgccagcaaa
     3061 atcaccaata tcctggacgg cacagtcact gcaaccagtt ccgatgccat taacggtagc
     3121 cagctttatg acttaagcag caatatcgcc acctacttcg gcggcaatgc ttctgtgaat
     3181 actgacggtg tgtttaccgg tccaacctac aaaatcggtg aaacaaatta ttataacgtc
     3241 ggcgatgcac tggctgcgat taactcctca tttagcacgt ctctcggcga tgctctgctt
     3301 tgggatgcca ccgcaggtaa attcagtgcc aaacacggta ctaatggtga cgcaagcgtg
     3361 atcactgatg tcgcagatgg tgaaatttca gactccagtt ctgacgcagt aaacggctca
     3421 caactccacg gcgtgagcag ttatgttgtt gatgcgctgg ggggggggtg ccgaagtcaa
     3481 tgcagacggc accatcactg cgccgacgta caccattgct aatgctgatt acgataatgt
     3541 cggtgatgcc ctgaatgcta tcgataccac tcctgacgac gctctgctct gggatgcgga
     3601 cgccggtgaa aatggtgcat ttagcgccgc tcacggaaaa gataaaactg ccagtgtaat
     3661 cactaacgtc gctaacggtg caatctctgc tgccagcagc gacgcgatta acggctcaca
     3721 actctatacc accaataagt acatcgctga tgcgctgggt ggtgacgcag aagtcaacgc
     3781 tgacggcacc atcaccgcac cgacttacac cattgcgaac gccgagtaca acaacgtcgg
     3841 tgacgccctg gatgcgcttg atgataacgc cctgctgtgg gatgagactg ccaatggcgg
     3901 tgctggagcc tacaatgcca gccatgacgg taaagccagc atcatcacta atgtcgctaa
     3961 tggcagtatt agtgaggaca gtaccgatgc agtgaacggt tctcagttga atgcgacgaa
     4021 tatgatgatt gagcagaaca cccaaattat caatcagctc gctggtaaca ccgacgcaac
     4081 ctatatcgaa gaaaatggcg ctggtatcaa ctacgttcgt actaacgaca acgatttagc
     4141 ctttaacgat gcaagcgcct ctggtgtcgg cgctacagct gtaggttata acgctgtcgc
     4201 gtctggtgcc agcagcgtag ccattggtca gaacagcagc agcaccgttg ataccggtat
     4261 tgcgctgggt agcagctccg tttccagccg tgtgattgcc aaaggttctc gtgacactag
     4321 cgtaacggaa aatggcgtgg tgattggtta tgacaccact gacggcgaac tgctaggtgc
     4381 attgtcaatt ggtgatgacg gtaaataccg ccaaatcatc aacgtagctg atggttcaga
     4441 agcccatgac gccgttacgg ttcgccagtt gcagaacgct attggagcgg tcgccactac
     4501 gccaaccaag tactttcacg ccaactcaac ggcagaagac tcactggccg ttggtgaaga
     4561 ctcgctggca atgggtgcga aaactgtcgt taatggtaat gcaggtattg gtatcggttt
     4621 gaacactctg gttctggctg atgcgatcaa cggcattgct atcggcagta acgcacgcgc
     4681 aaaccatgca aacagtatcg caatgggtaa cggttctcag accacccgtg gtgcacagac
     4741 tggctacacc gcctacaaca tggacgcacc gcagaactct gtaggtgagt tttctgtcgg
     4801 tagtgaagac ggtcaacgtc agatcacaaa cgtcgcagct ggttcagcgg ataccgatgc
     4861 ggttaacgtg ggtcagttga aagtcactga tgagcgcgta gcgcaaaata cccagagcat
     4921 tactaacctg aacaatcagg tcactaatct ggatactcgc gttactaata tcgaaaacgg
     4981 tattggcgac attgtcacca ccggtagcac caagtacttc aagaccaaca ccgatggcgt
     5041 agatgccaac gcccagggta aagatagcgt tgctattggt tctggttcca ttgctgccgc
     5101 tgacaacagc gtcgcactgg gtaccggttc cgttgcagag gaagaaaata caatctctgt
     5161 aggttcttct actaaccaac gccgtatcac caacgtagct gccagtgtta atgccaccga
     5221 tgcggttaac gtgtcgcagc tgaaatcttc tgaagcaggc ggcgtgcgct acgacaccaa
     5281 agctgatggt tctatcgact atagcaatat caccctcggt ggtggcaacg gcagtacgac
     5341 tcgtatcagc aacgtctccg ctggcgtcaa caacaacgac gcggtgaact acgcgcagtt
     5401 gaagcaaagc gcgcaggaaa cgaagcaata caccgatcag cggatggttg agatggataa
     5461 caaactgtct aaaactgaaa gcaagttgag cggtggtatc gcttctgcaa tggcaatgac
     5521 cggtctgccg caggcttata caccgggtgc cagcatggct tctattggtg gcggtactta
     5581 caacggtgaa tcggcagttg ctttaggtgt atcgatggtg agcgccaatg gtcgttgggt
     5641 ctacaaatta caaggtagta ccaatagcca gggtgaatac tccgccgcac tcggtgccgg
     5701 tattcagtgg taatgatcca ttaagttagt gtgactaagg gcaaacgcca aattgcctga
     5761 tgcgttacgc gcatcaggca agataaggcg ctccgggtca acaatctaag ggcaattctc
     5821 tgatgaggat tgcccttttc tttatcagac atctcccccc acaagaattg gccctaccaa
     5881 ttcttcgctt atctgacctc tggttcacaa ttccccaatt aaaactcaca tcaatattgc
     5941 caacacataa catttagtta accattcatt gtcattatcc ctacacaaca caattggcag
     6001 cgccactttt acacaacgtg tgacaaggag atgagcaaca gactcattac acgatgtgcg
     6061 tggactctag gagacctgca atgaatctct ggcaacaaaa ctacgatccc gccgggaata
     6121 tctggctttc cagtctgata gcatcgcttc ccatcctgtt tttcttcttt gcgctgatta
     6181 agctcaaact gaaaggatac gtcgccgcct catggacggt agcaatcgcc cttgccgtgg
     6241 ctttgctgtt ctataaaatg ccggtcgcta acgcgctggc ctcggtggtt tatggcttct
     6301 tctatggttt gtggcccatc gcgtggatca ttattgcagc agtgttcgtc tataagatct
     6361 cggtgaaaac cgggcagttt gacatcattc gctcgtctat tctttcgata acccctgacc
     6421 agcgtctgca aatgctgatc gtcggtttct gtttcggcgc tttccttgaa ggagccgcag
     6481 gctttggcgc accggtagca attaccgccg cattgctggt cggcctgggt tttaaaccgc
     6541 tgtacgccgc cggactgtgc ctgattgtta acaccgcgcc agtggcattt ggcgcgatgg
     6601 gcattccaat cctggttgcc ggacaagtaa caggcatcga cagctttgag attggccaga
     6661 tggtggggcg tcagctaccg tttatgacca ttatcgtgct gttctggatc atggcgatta
     6721 tggacggctg gcgcggtatc aaagagacgt ggcctgcggt cgtggttgca ggcggctcgt
     6781 ttgccatcgc tcagtacctt agctctaact tcattgggcc ggagctgccg gacattatct
     6841 cttcgctggt atcactgctc tgcctgacgc tgttcctcaa acgctggcaa ccagttcgcg
     6901 tattccgctt cggtgacttg ggggcgtcac aggttgatat gacgctggcc cacaccggtt
     6961 acaccgcggg tcaggtgtta cgtgcctgga caccgttcct gttcctgacc gctaccgtaa
     7021 cactgtggag catcccgccg tttaaagccc tgttcgcatc gggtggtgcg ctgtatgagt
     7081 gggtgatcaa cattccggtg ccgtacctcg ataaactggt tgcccgtatg ccaccagtgg
     7141 tcagcgaggc tacagcctat gccgccgtgt ttaagtttga ctggttctct gccaccggca
     7201 ccgccattct gtttgctgca ctgctctcga ttgtctggct gaagatgaaa ccgtctgacg
     7261 ctatcagcac cttcggcagc acgctgaaag aactggctct acccatctac tccatcggca
     7321 tggtgctggc gttcgccttt atctcgaact attccggact gtcatcaaca ctggcactgg
     7381 cactggcgca caccggtcat gcattcacct tcttctcgcc gttcctcggc tggctggggg
     7441 tattcctgac cgggtcggat acctcatcta acgccctgtt cgccgcgctg caagccaccg
     7501 cagcacaaca aattggcgtc tctgatctgt tgctggttgc cgccaatacc accggtggcg
     7561 tcaccggtaa gatgatctcc ccgcaatcta tcgctatcgc ctgtgcggcg gtaggcctgg
     7621 tgggcaaaga gtctgatttg ttccgcttta ctgtcaaaca cagcctgatc ttcacctgta
     7681 tggtgggcgt gatcaccacg cttcaggctt atgtcttaac gtggatgatt ccttaatgat
     7741 tgttttaccc agacgcctgt cagacgaggt tgccgatcgt gtgcgggcgc tgattgatga
     7801 aaaaaacctg gaagcgggca tgaagttgcc cgctgagcgc caactggcga tgcaactcgg
     7861 cgtatcacgt aattcactac gcgaggcgct ggcaaaactg gtgagcgaag gcgtgctgct
     7921 cagtcgacgc ggcggcggga cgtttattcg ctggcgtcat gacacatggt cggagcaaaa
     7981 catcgtccag ccgctaaaaa cgctgatggc cgatgatccg gattacagtt tcgatattct
     8041 ggaagcccgc tacgccattg aagccagcac cgcatggcat gcggcaatgc gcgccacacc
     8101 tggcgacaaa gaaaagattc agctttgctt tgaagcaacg ctaagtgaag acccggatat
     8161 cgcctcacaa gcggacgttc gttttcatct ggcgattgcc gaagcctcac ataacatcgt
     8221 gctgctgcaa accatgcgcg gtttcttcga tgtcctgcaa tcctcagtga agcatagccg
     8281 tcagcggatg tatctggtgc caccggtttt ttcacaactg accgaacaac atcaggctgt
     8341 cattgacgcc atttttgctg gtgatgccga cggggcgcgt aaagcaatga tggcgcacct
     8401 tagttttgtt cacaccacca tgaaacgatt cgatgaagat caggctcgcc acgcacggat
     8461 tacccgcctg cccggtgagc ataatgagca ttcgagggag aaaaacgcat gattatttcc
     8521 gcagccagcg attatcgcgc cgcagcgcaa cgcattctgc cgccgttcct gttccactat
     8581 atggatggtg gtgcatattc tgaatacacg ctgcgccgca acgtggaaga tttgtcagaa
     8641 gtggcactgc gccagcgtat tctgaaaaac atgtccgact taagcctgga aacgacgctg
     8701 tttaatgaga aattgtcgat gccggtagca ctggcaccgg tgggtttgtg tggcatgtat
     8761 gcgcgtcgtg gcgaagttca ggcagccaaa gcggcggacg cgcatggcat tccgtttact
     8821 ctctcgacgg tttccgtttg cccgattgaa gaagtcgcgc cagccatcaa gcgcccaatg
     8881 tggttccagc tttatgtact gcgcgatcgc ggctttatgc gtaacgcgct ggagcgagca
     8941 aaagcagcgg gttgttcgac gctggttttc accgtggata tgccgacacc gggcgcacgc
     9001 taccgtgatg cgcattcagg tatgagcggc ccaaatgcgg caatgcgccg ctacttgcag
     9061 gcggtgacgc atccgcaatg ggcgtgggat gtgggcctga acggtcgtcc acatgattta
     9121 ggtaatatct cagcttatct cggcaaaccg accggactgg aagattacat cggctggctg
     9181 gggaataact tcgatccgtc catctcatgg aaagaccttg agtggatccg cgatttctgg
     9241 gatggcccga tggtgatcaa agggatcctc gatccggaag atgcgcgcga tgcagtacgt
     9301 tttggtgctg atggaattgt ggtttctaac cacggtggcc gccagctgga cggtgtactc
     9361 tcttccgctc gtgcgttgcc cgctattgca gatgcggtga aaggtgatat cgccattctg
     9421 gcggatagcg gaattcgtaa cgggcttgat gtcgtgcgta tgattgcgct cggtgccgac
     9481 accgtactgc tgggtcgtgc tttcctgtat gcgctggcaa cagcgggcca ggcgggtgta
     9541 gctaacctgc taaatctgat cgaaaaagag atgaaagtgg cgatgacgct gactggcgcg
     9601 aaatcgatca gtgaaattac gcaagattcg ctggtgcagg ggctgggtaa agagttgcct
     9661 acggcactgg ctccgatggc gaaagggaat gcggcatagt cgtttgcgcc ccctcaccct
     9721 aaccctctcc ctcagggaga ggggaccgtt cggcgctgta tgtactccct cactctgaaa
     9781 cgacaccgca ctcttttttt ctccctcgcc cctccgggga gggccggggt gaggggaaaa
     9841 ggccgcacgg ttcaccaatt ttctgctatc ctgcccccgc actaaggggg cagtatgcta
     9901 aacatcgtac tttacgaacc agaaattccg ccaaatactg gcaacatcat ccgtctttgc
     9961 gctaataccg gctttcgtct acatatcatc gaaccgatgg gatttgcctg ggacgataag
    10021 cgcctgcgcc gcgcggggct ggactatcac gagtttaccg ccgttacgcg tcatcatgac
    10081 tatcgcgcgt tcctcgaagc agaaaatccc cagcgcctgt ttgccctcac cacgaaaggt
    10141 acgcctgctc acagcgccgt aagctatcag gatggcgatt atctgatgtt cggcccggaa
    10201 acacgcggcc tgccagcgag cattcttgat gccctgcccg ctgaacaaaa aattcgcatt
    10261 ccgatggtgc cggacagccg cagcatgaat ctgtccaatg cggtgtcggt agtagtgtat
    10321 gaagcctggc ggcagctggg atatccggga gcgttgttga gagattagtt actgtatgcc
    10381 ggatagatga ttacatcgca tccggcacga tcacaggaca ttagatccca tccccatact
    10441 caaatgtatg gttaataccg ttgaaatgct ggtccatatc cattgatggc ttatcgctgt
    10501 ctggtttacc gacaatacga gccggaacgc cagcggcggt tgtatgcggc ggcaccggtt
    10561 gcagcaccac ggaacctgcg ccaatcttcg cgccgcgacc aacttcaata ttgccgagga
    10621 ttttcgcgcc cgcgccaatc atcacacctt cacgaatttt cgggtgacgg tcaccaccag
    10681 atttacccgt accgccaagc gtcacagatt gcagaatcga tacgtcgttt tcaattaccg
    10741 ccgtttcacc cacaacgatg cccgtggcgt ggtcgagcat aatgccacgg ccaatttttg
    10801 ctgccgggtg aatatcgacc tggaacgtca cagaaacctg gttttgcaga aagattgcca
    10861 atgcgcgacg cccctgattc cacaaccagt gaccgatgcg ataggcctgc aaggcatgaa
    10921 aacccttcag gtataacaac ggggttgagt atttatcgac tgccgggtcg cgggtacgca
    10981 ccgcctgaat atcacaggcc gcagaggcga tcatttccgg gtcagcggcg taggcttctt
    11041 caaccacttc acggatagaa atagcaggca taattggcga tgacagcttg ttcgccagca
    11101 tgtagctcag tgcactgcca aggttttcgt gcttgagtag cgtcgcgtgg taaaaactgg
    11161 ccagcattgg ctcacagtcc gccagcgttc tggcttcggc tttaatattg ttccagacaa
    11221 tttccagttc ttcacacgac attgcttact ccacacgatg agataatgac cggcccgttc
    11281 tgcgcgggcc gggtcatagc ggtaacaaag gttccctggg gttagtggct gctgcgctcg
    11341 tccttgcgtg cacgacctaa taatgtcaat gctgcctcgc gcgcgttttt tccgcaatat
    11401 aatacttgat aaatttcctc ggttattggc atttcaacgc cgaagcgatg cgccagttcg
    11461 cggacttctt tcgtattgcg gtagccttcc accacctgac caatcttctc ctgcgcgctt
    11521 tgtacatcca tgccctgacc gagcatcatg ccaaaacggc ggttacgcga ctggttgtcg
    11581 gtacaggtaa gcaccagatc gcccagcccc gccatgccca taaaggtggc aggatcggca
    11641 cccagcgccg caccaagacg cgacatttca gccagcccac gggtgatcag cgccgtacgc
    11701 gcattcgcac caaaaccgat accgtcagac atccccgcgc caatggcaat gacgtttttc
    11761 accgcgccgc caagctgcac gccaatgaaa tccgggttgc tgtaaacgcg gaaacttttg
    11821 ccgcagtgca gcagctgctg gagatcatcg gcaaaggtct gatcggtcga ggccagcgaa
    11881 atagctgtcg gtaaacctgc cgccagttct ttcgcaaacg ttgggccaga gataaccgcc
    11941 agcggaattt gatcgcctaa ggcctcacgc gccacgtcct gtaacagacg tccggtttcc
    12001 gcttccagcc ctttggtcgc ccacaccaga cgcgcatcag gacgcatcag cggtttaatc
    12061 tggcgcagca cttcaccaaa gacatggctg ggtacgacga cgagaatatt acgactggct
    12121 gccagcgcag tggcgagatc gctttcaaga tggagcgtat cgggaaaagg cacatcgggg
    12181 agaaacgcgg cgttacagcg gtcgcgttca agcgttgcga tatgttcagg gtcatggccc
    12241 cagaggacaa cctcgtggcc atttcttgcc agggtaatgg caagagcggt gccgtacgag
    12301 ccggcaccga tcacagtcat tgaagcatta cgttggttca tcaggcatcc tgatgttctt
    12361 cagtaccttc gccagcctgc tgctgcaaat agttcatgaa cagcgcatcg aagttaaccg
    12421 gcgcaaggtt cagttgcggg aatgtaccgc gggataccat gctggtgatg cactcacgag
    12481 catacgggaa cagaatgttc gggcagtatg cgcccaggca atgtgccatc tgggtgcctt
    12541 cgatacccgc gatggagaaa ataccgccct gctgaacttc acacaggaac gcggtttctt
    12601 cgcccaagga ggccgttacg gtaacacgca gtaccacttc gtatacgtca tctgccagtt
    12661 gggaagatgc cgtatccaga tcaagtttaa cttctggttg ccaatctttc tggaaaacgt
    12721 gcggcgcgtt cggcgcttcg aaagagatat ccttggtata aatacgttgg atctggaaag
    12781 tcatttcagt gttgttttgt tctgacatgt gtagaaaacc cttaagtgtt gtccttaaat
    12841 actgcgcaat gtaatgccaa cgttcacacg ttatttcagc aggggatcca gtccaccacg
    12901 tgcatccaat gcatacaagt catcacagcc gccaatgtgc tgtgcgtcaa taaaaatctg
    12961 gggaaccgtg gtgcgaccgc tgcgtttgat catctcttca cgcttggcgg cattgccatc
    13021 gatcggcaac tcctggaaac tcacgccctt gctgctcagc agtgcttttg cacgatggca
    13081 atacgggcag gtttctttgg tatagatttc aacattggcc ataatttagc tcctgttttt
    13141 ttaccctgaa atttcatgtt gcagggcggc tgcaaaatgg agtgtcccca gaagcttata
    13201 acaataagtc gctgggggcc taattgcagc taacggcctg catcatgaaa gacgacaggt
    13261 aaattatttg ccgcgcacca gaggcaagtt ttcgccagcc cagccagcaa cgccctcttt
    13321 cagcacgaat acttgcgcaa aaccagcttt cgtcagcgcg tttgcaggct cctggcactg
    13381 catgccagaa ccgtctacca cgataaccgg tttgtctttg tgcttctcaa gctcaccaac
    13441 attgttggct ttgatttcgc tcggcaacag attgatagaa cctgcgatat ggcctttacg
    13501 gaagtcatca cgctgacgta aatccacaac cacagcgtct tctttgttga tcagacgcgt
    13561 agcttcacca cgagtaatca ccttcacttt cgaggtcagg ctcttaaacg tagtcacaag
    13621 aaccgccacc agtaacgcga tccaggcgat actcagtatg ggatgacggc caacaaattg
    13681 cataatttct tgcatggggg gtaacaactc ccgacgtagt gattaaaaaa accaggaaag
    13741 gagtatacct gcgcggtgcg gcaaatacag ccagcgcgtt aactggaatg caattttgcg
    13801 gggcacgacg aaaaaaaagc gcaaatctgg catcggcttg ccgcgagcgg tcgtatattt
    13861 tgatcttcag aggctatttt atcgattcag ctgtagtaaa attacgcaaa ttttgactct
    13921 tgagtatgag gttgtcgcaa tgtcggtttc taaaaaacct atggtactgg tgattctgga
    13981 tggctatggc tatcgtgaag aacaacagga taacgccatt tttagtgcta aaaccccggt
    14041 aatggatgca ctgtgggcca atcgtccgca taccctgatc gacgcttccg gtctggaagt
    14101 cggtctgcct gaccgtcaga tgggtaactc cgaagtaggc cacgttaacc tgggtgccgg
    14161 ccgcatcgtg tatcaggacc tgactcgtct ggacgttgaa atcaaagatc gcgctttctt
    14221 tgctaatcca gtgctggctg gcgcggtaca taaagcgaaa aacgcaggta aagcagtaca
    14281 cattatgggt ctgctctccg caggcggcgt acacagccac gaagatcaca tcatggcgat
    14341 ggtagaactg gcagctgaac gcggcgcaga aaaaatctac ctgcacgcat tccttgacgg
    14401 tcgcgacacc ccgccgcgca gtgctgaatc ctcgctgaaa aaatttgaag agaaatttgc
    14461 tgcgctgggt aaaggccgcg tagcgtccat cattggtcgt tactacgcga tggaccgcga
    14521 taaccgttgg gatcgcgttg aaaaagccta tgacctgctg actctggcgc agggtgagtt
    14581 ccaggccgat actgccgttg ctggtttgca ggcagcttat gctcgcgacg aaaacgacga
    14641 gttcgtgaaa gcgaccgtta ttcgtgcaga aggtcagcca gatgcggcaa tggaagacgg
    14701 cgatgcgctg attttcatga acttccgtgc tgaccgcgcg cgtgaaatca ctcgtgcatt
    14761 cgtgaacgct gatttcgatg gcttcgcgcg taagaaagtg gttaacgtcg atttcgtgat
    14821 gctgaccgaa tacgccgctg acatcaaaac tgcggttgct tacccacccg cttccctggt
    14881 taacaccttc ggcgagtgga tggcgaaaaa cgacaaaact cagttgcgta tttccgaaac
    14941 cgaaaaatat gcccacgtta ctttcttctt caacggtggc gtagaagagt cgttcaaagg
    15001 cgaagatcgc attctgatca actcaccgaa agtggctacc tacgatctgc aaccggaaat
    15061 gagctccgca gagctgaccg aaaaactggt tgcggccatc aagagcggca aatacgacac
    15121 cattatctgt aactatccga acggcgacat ggtaggtcac accggggtga tggaagcggc
    15181 ggttaaagca gttgaagcac tggatcactg cgtggaagaa gtcgcgaaag cggttgagtc
    15241 cgtgggtgga caactgctga tcaccgctga ccacggtaac gctgaacaga tgcgcgatcc
    15301 ggcaacgggt caggcacaca cggcacacac caacctgcca gttccgctga tttacgttgg
    15361 tgataagaac gtgaaagcgg ttgcaggcgg caaactttct gacatcgcgc cgaccatgtt
    15421 gtcgctgatg ggtatggaaa tcccgcaaga gatgactggt aagccgctgt tcatcgtgga
    15481 ataatccctc cccatgaggg gaaaggcgat taataccatg acacgggccg tgaaaccgcg
    15541 caggtttgca atcaggccca tcatctacgc cagcgttctt agcgctggcg tattgttgtg
    15601 cgccttttcc gcccacgcgg atgagcgtga ccaactcaaa tctattcagg ccgatatcgc
    15661 cgcaaaagag cgcgcggtac gccaaaagca acaacaacgc gcaagcctgc tcgcacaatt
    15721 gaaaaagcag gaagaagcga tctctgaagc cacccgtaag ctgcgcgaaa cgcaaaacac
    15781 gctcaatcaa ctcaataaac agattgatga gatgaacgcg tcgattgcca aactggagca
    15841 gcaaaaagcc gcccaggagc gcagcctcgc cgcacaactg gatgccgcat tccgtcaggg
    15901 cgagcatacc ggtattcagc taattctcag cggtgaagaa agccagcgtg gacagcgttt
    15961 acaggcttat ttcggctatc tcaaccaggc gcgacaagaa accattgccc agttgaagca
    16021 aacgcgtgaa gaagtcacca tgcagcgtgc tgaactggaa gagaaacaaa gcgagcaaca
    16081 aacgctgtta tatgaacagc gcgcccaaca ggcgaaactg actcaggctc tgaacgagcg
    16141 taaaaagacg ctggcggggc tggaatcttc catccagcaa ggtcagcagc agctaagtga
    16201 actgcgtgcc aacgaatccc gtctgcgtaa cagcattgcc cgtgcggaag ccgcggcgaa
    16261 agcgcgtgct gaacgagaag cgcgtgaggc ccaggcggtt cgcgagcgcc agaaagaagc
    16321 gacgcgcaaa ggcaccacct acaaaccgac cgaaagcgaa aaatcgctga tgtcccgaac
    16381 tggtggcctg ggtgcgccgc gtggtcaggc attctggccg gttcgcgggc cgacgctgca
    16441 tcgctatggc gagcagctac agggtgaatt acgctggaaa gggatggtta tcggtgcctc
    16501 tgaaggtact gaagttaaag caattgccga cggtcgagtg attctggctg actggctgca
    16561 aggttacggt ctggtggtgg tggttgagca cggtaaaggc gacatgagtc tttacggcta
    16621 taatcagagc gcactggtga gcgttggttc gcaggttcgc gcgggccagc caattgcact
    16681 ggtgggcagc agtggcggtc agggtcggcc ttcactctat ttcgaaattc gccgccaggg
    16741 tcaggcggtc aatccacagc cgtggttggg aagataagtt ttgtttccat ttcgtcgtaa
    16801 cgttcttgca tttgccgctc tgttggcgct ctcctccccc gtacttgctg gcaaacttgc
    16861 catcgtcatt gatgattttg ggtatcgccc gcacaacgaa aaccaggtgc tggcgatgcc
    16921 ttccgctatc tccgtcgctg tattacccga ttcaccgcac gccagagaga tggcgaccaa
    16981 agcgcataac agcggtcacg aagtgttgat tcatctcccg atggcaccgt tgagtaaaca
    17041 gccgctggag aaaaatacgc tacgcccgga gatgagcagc gacgaaattg agcgcattat
    17101 tcgtagtgcg gtcaataacg tgccctatgc cgtggggatc aacaaccaca tgggcagcaa
    17161 gatgacctct aacctgtttg gtatgcagaa agtgatgcag gcgctggagc gttacaatct
    17221 ttacttcctc gacagcgtaa ccatcggtaa tacccaggcg atgcgcgccg cgcaaggtac
    17281 tggcgtgaag gtgatcaaac ggaaggtgtt cctcgacgat tcgcagaacg aagcggacat
    17341 ccgtgtgcaa tttaatcgcg caattgatct ggcgcgtcgc aacggttcaa ccattgccat
    17401 tggacatcct cacccttcaa cggtacgcgt gctacaacag atggtttata acctgccgcc
    17461 agacattacg ctggtgaaag ccagcagctt gctgaatgaa ccgcaggttg atacttctac
    17521 accgccgaaa aacgctgtgc ctgatgcacc gcgtaatccg ttccgtggcg tgaagctgtg
    17581 caaaccgaag aaaccgatag aacctgttta tgctaatcgc ttctttgaag tattaagcga
    17641 aagcatcagc cagagcacgc tgatcgttta cttccagcat cagtggcaag gctggggcaa
    17701 acagcccgaa gcggcgaagt ttaacgctag cgcaaattaa aggcgctacg cgtaatgcga
    17761 tggtgcgacc gcgttttgtc gcgccattgc cataagcgga aagaccacag taacgcctgg
    17821 taaccgacct tcacgctgcg cacattggta atcaggcgct tatacatacc ggaagtaaat
    17881 atctcggcaa tcatccgttg gcgggtaaga atatccggct ctttgcgcac cgcatggcaa
    17941 acacgcaatg cttcgtaagt tatttgctga tgaaattccg gataaatcgt aattttgtcg
    18001 gcataatttc gatttaattt ctccagcagg cgggtaatct taatatagtg acgttgataa
    18061 ttaaggtttt tattcccttg tctatgcaac cgactcactg acgtattatg cagataatat
    18121 ttatataatg attgctcggt atatcgcgca cgcagcgcgt taaacatgaa ttctgttgtc
    18181 cagacaatat cctgatgatg taatccggca ataaatttaa tgttattttt aacaataaca
    18241 tcacggcgat aaacccccat ccagacaacg tgagtccaac ggcgcgaaaa aagccccatc
    18301 cgcagccagt ccgggccggt taatacaccg gttgagcgaa ggcgatcgat ggggatggat
    18361 tgccaagttt ctcccgtttc acgaaaagac cagtcagcgt tgcactgcgc cacgtcgagg
    18421 tcgtcctcta acgccatggt catcagcgtt tcgtacatgg tgggatagac ctcatcgtca
    18481 gcatcgacaa aagcgacata tttgcccgtc gccacctcga tcccacgatt acgcgctacc
    18541 gatgcgccag cattcgcctg atgcaacaaa cgaacgtgcg gatagttttc tgcgtaatgc
    18601 tttgctattt caacagaatt atccgttgaa ccatcgttaa taataatgat ttccagagca
    18661 gtccaggttt gcgtaattaa agattccata caagtgcgga aatcatcgcc cgcattatat
    18721 aacggaataa taacactaag tttattggtg ctgttcatca taaattaccg acgacctctg
    18781 aagttatcct gacgttttac aggctttgta ttaagaaact attaagcctg tgagatatta
    18841 tttttacagg tgagagagtc acttcagggt agagagataa tgagaacagc tatataaaag
    18901 atcagcgcaa ttacgaactc tttattacac tgacagga
//
LOCUS       DADLSA010000075        18564 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.075, whole genome shotgun sequence.
ACCESSION   DADLSA010000075 DADLSA010000000
VERSION     DADLSA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.075"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(193..287)
                     /locus_tag="JN691_002516"
     tRNA            complement(193..287)
                     /locus_tag="JN691_002516"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(251..253),aa:Sec,seq:tca)
     gene            580..1963
                     /locus_tag="JN691_002517"
                     /pseudo
     CDS             580..1963
                     /locus_tag="JN691_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            1973..4291
                     /gene="yicI"
                     /locus_tag="JN691_002518"
     CDS             1973..4291
                     /gene="yicI"
                     /locus_tag="JN691_002518"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAY7243934.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFCFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(4344..6053)
                     /locus_tag="JN691_002519"
     CDS             complement(4344..6053)
                     /locus_tag="JN691_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY7243935.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTSLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(6174..7565)
                     /gene="xanP"
                     /locus_tag="JN691_002520"
     CDS             complement(6174..7565)
                     /gene="xanP"
                     /locus_tag="JN691_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAY7243936.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            7845..9050
                     /gene="gltS"
                     /locus_tag="JN691_002521"
     CDS             7845..9050
                     /gene="gltS"
                     /locus_tag="JN691_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAY7243937.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(9084..11165)
                     /gene="recG"
                     /locus_tag="JN691_002522"
     CDS             complement(9084..11165)
                     /gene="recG"
                     /locus_tag="JN691_002522"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAY7243938.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(11171..11860)
                     /gene="trmH"
                     /locus_tag="JN691_002523"
     CDS             complement(11171..11860)
                     /gene="trmH"
                     /locus_tag="JN691_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAY7243939.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAAQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(11867..13975)
                     /gene="spoT"
                     /locus_tag="JN691_002524"
     CDS             complement(11867..13975)
                     /gene="spoT"
                     /locus_tag="JN691_002524"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAY7243940.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(13994..14269)
                     /gene="rpoZ"
                     /locus_tag="JN691_002525"
     CDS             complement(13994..14269)
                     /gene="rpoZ"
                     /locus_tag="JN691_002525"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAY7243941.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQESAELQAVTAIAEGRR"
     gene            complement(14324..14947)
                     /gene="gmk"
                     /locus_tag="JN691_002526"
     CDS             complement(14324..14947)
                     /gene="gmk"
                     /locus_tag="JN691_002526"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAY7243942.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            15205..16887
                     /gene="ligB"
                     /locus_tag="JN691_002527"
     CDS             15205..16887
                     /gene="ligB"
                     /locus_tag="JN691_002527"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HAY7243943.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(16884..17501)
                     /locus_tag="JN691_002528"
     CDS             complement(16884..17501)
                     /locus_tag="JN691_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAY7243944.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(17792..>18564)
                     /gene="dinD"
                     /locus_tag="JN691_002529"
     CDS             complement(17792..>18564)
                     /gene="dinD"
                     /locus_tag="JN691_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HAY7243945.1"
                     /translation="AEQWSARQLGKLLGYSEYRHFIPVLTRAKEACENSGHTIDDHFE
                     EILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVIAAGQTYFTIQTRRQELAD
                     DEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATATDFAIFQNHGYQGLYGGLD
                     QKAIHQLKELKKSQKILDHMGSTELAANLFRATQTEEKLKRDSVNSKQQANTTHFDVG
                     SKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
BASE COUNT         4242 a         4705 c         5011 g         4606 t
ORIGIN      
        1 ccgttgttat gtatcacatt tttcctatta tgggggtgct tgtatttcaa taggaaataa
       61 caccccctaa agtaccccca aaagaaactt gatacggggt gaagtaggtt gacctcagga
      121 tacaaataaa tactgatata caagggatta gaagagattt attgacattg ggagagttca
      181 gaagaggtaa actggcggaa gatcacagga gtcgaacctg cccgggaccg ctggcggccc
      241 caactggatt tgaagtccag ccgcctcacc ggagacgacg atcttccgcg cctcgattgc
      301 tacatggagg cggggcgcat tatagctact tccttgagtt tctacatccc ccagatcagt
      361 tttttccgcc taataaacgc agcgtattat cacgaaagca ttataattca tataaaaccc
      421 atattttaca atgatatatg agcggaatta acgctaccac gatcacataa aacaagaatc
      481 ataaattaat aaccagatat cggaatattc gctctcacag ggatggttta caaaatgcgt
      541 tatcggtctc agcaccccta tagcatcaag gaaaagcaga tgaagagtga agtgttgtcc
      601 gttaaagaga aaattggtta tggcatggga gacgccgcca gccacattat tttcgataac
      661 gtaatgttat atatgatgtt cttttatacc gatatttttg gcattcctgc cggatttgtc
      721 ggaaccatgt ttttggtcgc tcgtgcactg gatgcgattt ccgatccttg catggggttg
      781 ttggccgatc gaacgcgctc tcgctggggt aaatttcgtc cgtgggtact gtttggcgca
      841 ctgccattcg ggatcgtctg tgtactggcc tatagcacgc cagatctcag tatgaacggc
      901 aaaatgatct atgcagcaat tacttacacc ctaacttacc ttactttata ccgtcgtcaa
      961 tatcccttac tgcgcattgg gtggtgtaat caccaatgac ccgactcagc gtatctcgct
     1021 gcaatcctgg cgttttgtgc tggcgaccgc gggaggcatg ctttctactg ttctgatgat
     1081 gccactggtt aatttaattg gcggtgataa taaaccactc ggtttccagg gcggtatcgt
     1141 ggtcctttcc gtggtggcat tcatgatgct ggcattttgt ttcttcacca ctaaagaacg
     1201 cgttgaagca ccacctacaa caacgtctat gcgggaagat ttacgtgata tctggcaaaa
     1261 cgaccagtgg cggattgtcg gtttactaac cattttcaat atcctagcgg tgtgcgtacg
     1321 cggtggggcg atgatgtatt acgtcacatg gattttgggc acgccggaag tgtttgtcgc
     1381 ttttctcacc acttattgcg tgggtaacct gattggttcc gcactggcaa aacctctgac
     1441 cgactggaaa tgtaaagtca ctatcttctg gtggacgaac gccctgctgg cagtgattag
     1501 cctcgcgatg ttctttgttc ccatgcaggc cagcatcact atgtttgtct tcatcttcgt
     1561 gattggtgtg ttgcatcaac tggtgacacc tatccagtgg gtaatgatgt ccgataccgt
     1621 cgactacggc gagtggtgca atggtaaacg cctgaccggg atcagttttg ctggcacgct
     1681 gtttgtgctc aaactggggt tggccttcgg cggcgctctt atcggctgga tgctggctta
     1741 tggcggatat gatgcggcag aaaaagcgca gaacagcgcc acgattagca tcattattgc
     1801 gctattcacg attgttccgg cgatctgtta tttgctgagc gcgattatcg ctaaacgcta
     1861 ctactcactc acgacgcaca atctgaaaac cgttatggaa cagctggctc agggtaaacg
     1921 ccgttgccag caacaattca cctctcaaga agtgcagaac taaggaacgg caatgaaaat
     1981 tagcgatgga aactggttga ttcaacctgg cctcaatttg attcacccgc ttcaggtatt
     2041 cgaggttgaa cagcaggata atgaaatggt ggtctatgct gccccccgtg atgtgcgtga
     2101 acgtacctgg cagcttgata cgcctttatt tacgctgcgc tttttctccc cacaggaagg
     2161 tattgtcggt gtgcggattg agcattttca gggggcgctg aataacggtc ctcattatcc
     2221 gctcaatatt ttgcaggatg tgaaggtcac aatcgaaaac acagaacgtt atgctgagtt
     2281 taaaagtggc aacttaagcg cgcgtgtcag caaaggtgag ttctggtcac tggattttct
     2341 gcgcaacggc gaacgtatta ccggtagtca ggtgaaaaat aatggctacg tgcaggacac
     2401 gaataatcaa cgcaattata tgtttgagcg gcttgatctt ggcgttggcg aaacagttta
     2461 cggtctggga gagcgcttta ctgccctggt gcgcaatggc cagacggtag agacctggaa
     2521 ccgggacggc ggcacaagta ctgaacaggc gtataaaaat atcccgttct acatgactaa
     2581 ccgtggttat ggggtactgg tcaatcatcc ccagtgtgtc tcttttgaag tgggatcgga
     2641 gaaagtctcc aaagtgcagt tcagcgttga gagtgaatat ctcgaatact ttgttatcga
     2701 cggcccgacg ccgaaagcgg tacttgatcg ttatacccgc tttactggtc gtccggcgct
     2761 gccgcccccg tggtccttcg gcctgtggct aaccacttca tttaccacca actacgacga
     2821 agcgacggta aacagcttta tcgatggtat ggcggaacgc aatctgccgc tgcatgtttt
     2881 ccactttgac tgtttctgga tgaaagcctt ccagtggtgc gattttgagt gggacccgct
     2941 gactttccct gacccggaag ggatgatccg ccgcctgaaa gcgaaagggc tgaaaatctg
     3001 cgtctggatt aacccctata tcggtcaaaa atcccccgtc tttaaagagt tacaagagaa
     3061 aggctattta ctcaaacgcc cggacggttc gctatggcag tgggataaat ggcagccagg
     3121 tctggcgatt tatgacttta ccaatccgga tgcctgcaaa tggtacgccg acaaactgaa
     3181 aggtctggtc gcgatgggcg ttgattgctt taagaccgac tttggcgaac gtatcccaac
     3241 tgatgttcag tggtttgacg gttccgatcc gcagaaaatg cataaccatt atgcgtacat
     3301 ctacaacgaa ctggtgtgga acgtgctcaa ggacaccgtt ggtgaggaag aagctgtctt
     3361 gtttgcccgc tcggcctccg tcggtgcgca gaaattcccg gtacactggg gtggcgattg
     3421 ttacgctaac tacgaatcaa tggcggaaag cctgcgcggt ggtttgtcta ttggcctttc
     3481 aggtttttgc ttctggagcc acgatatcgg cggctttgaa aataccgctc cggcgcacgt
     3541 ttacaaacgc tggtgcgcgt ttggtttgct ctccagccat agccgtttac acggtagcaa
     3601 atcttatcgt gtgccgtggg cctacgatga tgagtcctgt gatgtggtgc gcttcttcac
     3661 gcaactgaaa tgccgcatga tgccgtatct gtatcgtgaa gctgcgcgtg cgaacgcgcg
     3721 gggtacgccg atgatgcggg ccatgatgat ggagttcccg gacgatccgg cttgtgatta
     3781 ccttgaccgt caatacatgt taggcgacaa cgtgatggtt gcgccggtgt tcactgaagc
     3841 gggcgatgtg cagttctacc tgccggaagg tcgctggaca cacctgtggc acaacgatga
     3901 actcgacggt agtcgctggc ataaacagca gcacggcttc ctgagtctgc ccgtttatgt
     3961 gcgtgataac actctactgg cgctgggcaa caacgatcaa cgtcccgatt acgtgtggca
     4021 cgaaggcacg gcattccacc tctttaatct gcaagacggg catgaagccg tctgtgaagt
     4081 gcccgctgct gacggatcgg tgatctttac tttaaaagca gcacgtactg gcaacacgat
     4141 tactgtgact ggtgcgggcg aggcgaagaa ctggacgctg tgcttgcgca atgttgtgaa
     4201 agtaaatggt ctgcaaggcg gttcgcaggc tgaaagtgag caggggctgg tggtgacgcc
     4261 tcaagggaat gcgctgacaa ttacgttgta atctattgac ggaatgccgg gtacgtctga
     4321 gtcgtacccg gcgagattga tgtttaccgc accggctccg tcgatgtaac ttcaccgctg
     4381 gaaacgacgc ccgcattcat ggtttgagtc acttgctgct ttgcggcgtt cacggcatgc
     4441 aattgcccgc taaccgtagg tttcagcggg gcattggcct gaatatctcc actggcggta
     4501 agctgaatat tgccgtcgcc agtcaacggt aattcaggcc atccccattg ttgcaaaata
     4561 ttcaccggca caccgcgtcc attcaggctg atatgggtct gacgttgtgg cgtttgtgaa
     4621 acacttgcgg tggcttccag aatgcctttt tcagtaaatg cactcagttc gctgatattc
     4681 accgtgctgc tgttggcggt cagcgccagc gacgggcgac gaacatcaac acgattgaat
     4741 gtcgcggcgg cggcattcag attcgccgag ccactccaga cgccccattt atgatcggta
     4801 accagcgtca ggttggcacc gtaaccatcg agcgaggtga gctgccacgg gaagtcaggg
     4861 tcgatatcaa tgatcagatt gcggctggcg ctaaatctct tcagttgcag gctgtttaac
     4921 caaccgggtg tcgtttccat ccacaactgt tgccagtttt tcggcaaggt atattccagc
     4981 ccggcaattg ccgcatcatc aaggatcaac gttttaccgt cacgcagcca gttccctgac
     5041 gttctgacca tacccccttc ccagcggctg gtgaactggc gcagcgctac gccctgcggg
     5101 gaaaattccg cgtttataat cgggtcaaat aaatgcagcg aaccataaat gaactcgctg
     5161 gcgttcatcg acagtttgcc ttcctgtgtc tgccagtcat ctttactgaa ggtcatgttg
     5221 cgcaagctga gatcgaggtc ggtcaccgcc cagtccggac cttgcagacg agcatcgatg
     5281 acttccaagc gaccaatctg caacgacggg acagagcgta atggcgcaaa gaagtcggtt
     5341 agcgattttt cgctttgtag acggatatcc gccatgcgca gattttccac ttgccagctg
     5401 ccgtcggcgt tacgctgcgc gtttccggtt aatgtcccgc gggcgatgtc ggcacccagg
     5461 ttagtcagcg taacgcgatc gttatcaata ctgccttcaa tcagtacatt ggtggcagga
     5521 acatcattga gcgaaagcga tccggcacta aactgaatct gcgccttcgt acccagcact
     5581 ttaccggctt ctggtgacca cggaaccacg ccgccattta cccgctgcgc gctcagtttc
     5641 cattcgctat tcgggctatt aaacgccata tcacgcagtt gcagacgatc ggctttgaac
     5701 ggtagcggcg cggtctggtc ggtgagattc agcgtcccgt tttccagcag gatggtatcg
     5761 acatggcgtg gttcggtcag ttggcgactg cttagcgcaa tgtcgacact ttttgccacc
     5821 aggggcgcgg gctgaccatc acgaccaaac gtgacattct ccagcacgat atgagatggc
     5881 gcggaaaaac ggtgatccat cgccccgaag gccagatgat agtcgctatt ctcggaaacc
     5941 catgcgctga tatgttctgc tccccagcgg gtttgcagaa gaaaatagag gccagcgatc
     6001 accactaaca gagcgatgag aatgtagaga agcagcttcc caataaattt catggtcttc
     6061 catcccgtga aatgcacata agggagttat gcacgattta cgcgcaatcc tcaaggcggg
     6121 aatggtgaaa gaggtgagac tgcggcggta attatcttac cgccgccagt gaattactgt
     6181 ttttctggtg ggaaaatcag attcagcacg atggcagtaa taccgcccgc ggcgatcccg
     6241 gaggagagca ggtttttcag ccattcaggg gcaaactgca aaatcagcgg ctgctgagac
     6301 acgcccagac caaccgccag cgacagcgcg ataatcagaa tcgcccgacg gttcagcggc
     6361 tcacgagaaa cgatacgcac accggaggcg gcgatggtgc caaacattac aagcgttgcg
     6421 ccgcccagaa ccggttctgg aatgtgttgt acaaaaccgc tcactgccgg gaacagaccc
     6481 agcacgatca acatcagcgc gacgacaaaa ccgacatagc ggctggcaac accagtcaac
     6541 tggatcactc cgttgttttg cccgaagcag gagttcggga aggtgttaaa caccgccgaa
     6601 acaaacgagt tcaggccgtt tgccagcacg ccgcctttca ggcgtttcat gtacagcgga
     6661 ccggacactg gctgttcgga aacgtcagag gtcgccgtga tatcgccaat ggtttccagc
     6721 gaagtgatca taaagaccag catcagcggc agcagcagac tccattcaat gccaagaccg
     6781 taatagagcg gcgttggcac cataatcagt tcttgcgtca tcggttcgtt gctttctggc
     6841 aacatgccca taaaccacgc cagcgcatat ccggccgcca tcgcaattac cagtgaggcc
     6901 acgcgtaagt aagggttacg ttgacggtta agcaggataa ttaaggctaa aaccacgcct
     6961 gccagcagca gatttttcgg tgcgccgaag gtgttatcgc tcatggctgc gtaaccgccg
     7021 ccaatggagg tcagccccac ctgaattagc gacaggccga taatcatcac cacaacgcca
     7081 gaaaccagcg gcgtaataat gcggcgcgcc agatgcagaa cgcgggagat caccatctcg
     7141 gtgcaacttg ccagcatcaa cgtgccgaac aaagccgcca tcatggtagg aacatcagca
     7201 ccaccggttt tcagcgcggt accgcccata atcagcgggg caacaaagtt gaagctggtg
     7261 ccctgaatag acaacagccc ggagccaacc ggaccccagg ccttaatttg aataatcgat
     7321 gccacaccgg aggcaaacag cgacatacta ataatgtgtt gcgtgtcttg tgccggtaaa
     7381 cccagcgcct ggcagattaa tagcgctggc gtgatcaccg caacgaacat cgccagcaga
     7441 tgctgacagg cggcaaacag ggtttgagga agcggcggac gatcttcaag acggtaaatc
     7501 agttcgctgt tttgagtctg cgcaaccggt tgcgcatttt ctgactcgag ggtggaaaca
     7561 gacatcgaaa acaatcccgt ggtagcaaag cgggcatttt agctgaccga aagagaaaag
     7621 caaacgtttg ccacatcctc atctaaatat ttaaatgaga taaatgcact tttttatata
     7681 acttttggta tttttctgct taaaatccat gccatttgtt cattaacagc tgagaatttg
     7741 acccaggaaa gcataactcg atacccgtca tagttcatga agttgccact gcgcgatgaa
     7801 gtatgacgag tatgaaagag tgatgcggat acaaaggagt aactatgttt catctcgata
     7861 ctttagcaac gcttgttgcc gcaacgctga cgttgctgct cgggcgtaag ttggtccatt
     7921 ccgtctcctt tttgaagaaa tacaccatac cggaacctgt tgcgggtggt ttgttggtgg
     7981 cgctggcgct actagtactg aaaaaaagca tgggctggga agtcaacttt gatatgtccc
     8041 tgcgcgatcc gttaatgctg gctttcttcg ccaccattgg cctgaacgcc aacattgcca
     8101 gtttgcgtgc cggtgggcgt gtggttggca tcttcttgat tgtggttgtt ggtctgttgg
     8161 tgatgcaaaa tgccattggc attggtatgg ctagcctgtt agggcttgat ccgctgatgg
     8221 ggctgttggc cggttctatt acgctttccg gcggtcacgg tacgggcgct gcgtggagta
     8281 aattgttcat tgaacgttat ggcttcacca atgcgacgga agtggcgatg gcctgtgcaa
     8341 cgttcggtct ggtgctgggc ggcttgattg gcggtccggt ggcgcgctat ctggtgaaac
     8401 actccaccac gccgaacggt attccggatg accaggaagt cccgacggcg tttgaaaagc
     8461 cggatgtggg acgcatgatc acctcgttgg tgctgattga aactatcgcg ctgattgcta
     8521 tctgcctgac ggtggggaaa attgttgcgc aacttttggc tggcactgct tttgaactgc
     8581 cgaccttcgt ctgtgtactg tttgttggcg tgattctgag caacggtctg tcaataatgg
     8641 gcttttaccg cgtctttgag cgtgcggtat ccgtgctggg taacgtaagc ttgtcgttgt
     8701 tcctggcgat ggcgttgatg gggctgaaac tgtgggagct ggcttcgctg gcgctgccga
     8761 tgctggcgat tctggtggta cagaccatct tcatggcgtt gtatgccatc ttcgttacct
     8821 ggcgcatgat gggcaaaaac tacgatgcgg cagtgctggc tgcgggtcac tgtggttttg
     8881 gcctcggtgc aacgccaacg gcaatcgcca acatgcaggc gatcactgaa cgctttggcc
     8941 cgtcgcacat ggcgtttttg gtggtgccga tggtcggtgc gttctttatc gatatcgtca
     9001 atgcgctggt gattaagctg tatttaatgt tgccgatttt tgccggttaa ccgatgaagc
     9061 ggcggtcttc tcaccgccgc cttttacgca ttcgagtaac gttctgtctc cggcatccag
     9121 cgttctatca aggcttttgc ctgttgtggg taacgttcgt gaatatgtcg cgccaggcgc
     9181 tgaacttccg ggatcatcgc ctgatcgcgc agtaaatccg ccactttaaa ttcagcatta
     9241 cccgtctgac gcgtgcctaa caattcgcca gggccgcgaa tctccagatc tttttgcgca
     9301 atcacaaaac cgtcgttact gtcgcgcagc acttgcaggc gaatttgcgc cgttttagaa
     9361 agcggcgttt tgtagagcag cacgcagtga gaagccaccg cgccacgacc tacgcgcccg
     9421 cgcagctggt gtaactgcgc cagacccaga cgctcagggt tttcgataat catcaggctg
     9481 gcgttaggca catcaacacc gacttcaata acggtagtgg caaccagcag atgtagctca
     9541 ccttgtttaa acgacgccat caccgcctgt ttctcggctg gtttcatccg cccgtgtacc
     9601 aggccaacat tcaattctgg tagcgccagt ttcaactctt cccaggtagc ttccgccgcc
     9661 tgcgcttcca gtaactctga ctcttcaatc aacgtacaaa cccagtacgc ctgacgacct
     9721 tcagttatgc aggcgtggcg cacgcggtca atgatgtcgg tacggcgggt atcaggaata
     9781 gcgaccgtag tcactggcgt gcggcctggc ggcagctcat ctatcaccga ggtatcgaga
     9841 tcggcatacg ccgtcattgc cagcgtgcgg gggatcggcg tggcggtcat gatcaactga
     9901 tgcggatgga agccctgctg ctggcctttc tcccacaatg ccagacgctg atgcacgcca
     9961 aaacgatgct gttcgtcgat aatcaccagc gccaggccgt taaactgcac ctgttcctgg
    10021 aagatggcgt gtgtaccgac aatcatctgc acctgaccgc tggcgatggc ttcctgctga
    10081 gacagtcgcg ctttgccttt ctgcttaccg gcgagccagc ccacttcgat accgagcggt
    10141 gcaaaccagt tgcggaagtt attggcgtgc tgctcggcga gtaattcggt tggtgccatc
    10201 agtgccacct gtttgccgtg ggcaatcgca cgcagcgcgg cgagggcggc gaccagcgtt
    10261 ttaccggaac ctacatcgcc ctgtaccagg cgcatcatcg gcacatccag cgccatatca
    10321 tgctcgatct ccgccactac gcgtgcctgt gcgcccgttg gcttgaacgg taaggcggcg
    10381 aggagtttat ttttcagcgt gtcattggcg ctcagcggct gggcatgaaa acgctgtgct
    10441 ccggcacgta aggctaacat gctgaggttg tgcgccagca gttcttccag aatcagacga
    10501 cgttgcgccg gatgctgccc ggtttccaga tcgctaagct gtagcgtcgg tggcgggcgg
    10561 tgcaaagtgc gcaacgcttc cggtagcgtc atcattcctt gtgacagttc cggcggcagg
    10621 agttcttcaa tggcgcaggt gtcgagcaga tccagcgcct ggtcggttaa tttacgcagc
    10681 gtggcctgct ttacgccttc cgttgttgga taaaccggcg tgagcgtttc ctgtaattct
    10741 ggcgtgctga gatcgccctg cacgcggtat tccgggtgga tcatctccgc accatattta
    10801 ccgcgctttg cttcgccata agccagtaca cggcggcccg tcgccaggct atttttcatt
    10861 gccgcgctga aattgaaaaa gcgcatggtg aggatgccgg aaccgtcgct gatctggcag
    10921 gtcatcatcc gccgaccgcc gaaggagata ttgcagttca gcacttcgcc ttccaccgtg
    10981 gcataaacgc ccggcagtag ttctccgatg gggtagagat gggtgcgatc ttcgtagcgc
    11041 aggggaaggt gtaagagtag atcctgcacg gtatgcaggt tgattttcgc cagtttgtta
    11101 ctaagtgctg cgccaacgcc cgttagggaa ctgagtggga cagcatctaa caggcgacct
    11161 ttcatggcac ttaccctgca gcctgcatag tagcccacca gtcggcatca gcttcgatct
    11221 cgccttgctg attgacgtgg ggataaggca ggcctttgcg ttttgcgact ttcgccagca
    11281 ccggatagcc gccttcaaac aacaggcgtt gttgcgctgc ttccggcaac atgctgtttt
    11341 cacgcaggta catgcctgca ttttgccgct gacgctgggc ttcgtaaaga atgagggctg
    11401 aggcaacgga aacattcagc gactgcacca tgccgatcat cggaatgatg atgtcctgat
    11461 ccgccagggc caatgcttcc tgcgtgatgc ccgttttctc ctgtcccatc aaaatgcagg
    11521 tcgggcgagt gtaatcaatt tcgcggaaat cgacagcatt atcagaaaga tgggttgcca
    11581 gaatctgcat gccctggcct ttgagatgag cgacggcatc gccaatggtg cggtgtgttt
    11641 tcacctgtac ccagctgtta ctacccgccg ctgccgaagc catggtgcgc atgcggctac
    11701 caggccagac ggcgtgaact tcatgtacgc caacggcatc tgcggtacga ataatcgcag
    11761 aaacgttatg aggtttgtgg acctgctcca tgcagacggt cagatcaggc tgccgcctgg
    11821 cgagcatttc gcagatgcgt gcataacgtg ttgggttcat aaaacattaa tttcggtttc
    11881 gggtgacttt aatcacgtct ggcatcacgc ggattttgcg catgatattc gccagatgca
    11941 cacggtcacg agcggtcaga cgaataaagg cgctgtagac gcgaccatct ttctcttccg
    12001 tattcaaact ttgaatattc gaagtcgtgg tgttaattgc cgccgtcagg tttgccagcg
    12061 cgccctgatg attgaacatc tccaccttga tttcggtgat gaactcctgc gccgtctctt
    12121 tatcccattc cacagccata aacttctctg gctctttctg gtagccacgg atattacggc
    12181 aggattcatg gtggatcacc agacctttac cggggctgac gtgggcgata atcgggtcgc
    12241 caggaatagg gcggcagcat ttcgcaaagg tgatcagcac gccatcggca cctttaatgg
    12301 gcagatgtcc gtggctttgg gttgccggtg gaatggaggc gtccccatgt tgcagatttt
    12361 tcgcgaccac cacgctcatt gcgttaccaa gtccgatttc tgccagcaga tcgtcaagcg
    12421 ttgccagctt catgcgatcc agctcgcgct gaatattttc ctgcgggatt tcattcagct
    12481 tacggctacc acccaaagca tggttgagca gacgacggcc caggcttaca gaatcatcac
    12541 gcttgaggtt tttcagcaac tgacgaattt tggcgcgcgc ttttgagcta acgacaaagt
    12601 tcagccacgc ggcattcggg cgagcgcccg gtgcggtaat gatttcaacg gtttgaccgc
    12661 tggtaagcgg ctgcgacagc gggtaaggct ggcggtcaac gcgtgcgccc acgcaggcat
    12721 gaccgatatc ggtatgcact gcataagcga agtcgacggg cgttgcaccg gcaggcagct
    12781 cgacaattcg cccttccggt gtgaaaacgt aaatctcatc cgggaagaga tcggatttaa
    12841 cgctctcgat aaattcaaac gaactaccgg cgctctgttg cagctccagc aggctttgca
    12901 tccagcgctg ggcgcggatt tgcgcggtgg tgctggtttc gccgtgctct ttataagccc
    12961 agtgcgcggc aacacccatc tccgccatct gatccatatc ttcggtacgg atctggacct
    13021 caaccggcac accgtgcggg ccgatcatcg aggtgtgcaa agactgatag ccgttcgctt
    13081 ttggaatggc gatgtagtct ttcacgcgac ccggacgcgg cttgtacagg ctatgcattt
    13141 gcccgagcac gcgataacag gtgtcggaat cattgacgat cacgcggaaa gcgtagatgt
    13201 ccatgattga gtgaaaacgc tgctctttga gcaccatttt gcagtaaatc gaataaagat
    13261 gcttctcgcg accactgacg cggcagggaa ttcccgcttc ctgcaaacgc ccttcgattt
    13321 ccgagaggat tttttggatc atctctttac ggttgccgcg cgcggctttc accacttctt
    13381 tgattacgcg ataacggttg ggatacagcg cctcaaaacc cagctcttcg agttcggttt
    13441 taatgtggtg gatacctaaa cggtgcgcca gcgggctgta aatttcgaga gtttcacggg
    13501 cgatgcggcg acgtttgtcc gggcgaagtg agcccagcgt gcgcatgttg tgggtacggt
    13561 cggcaagttt gatgaggatg acgcggatat cctgcaccat cgccataatc atcttgcgaa
    13621 agttttcggc ctgcgcctct ttcttatcgc ggaacttgag tttatcaagt ttcgacaccc
    13681 cctctaccag ctcggcgacg cttttaccaa aaagctgttc catatcctgg taggtggcgg
    13741 gagtatcttc aatcacgtca tgcagcagcg ccgccatcag cgtttcatag tcgagtttca
    13801 tctcggccag aatgcaggca accgctaccg ggtgcgtgat atagggttca ccgcttgaac
    13861 gtgtttgccc ctcgtgagca tcacgtgcaa cgagatacgc ctgccgcaga cgcttgattt
    13921 ggtcttccgg caggtaggtt tgaatcagtt gattcaggct ttcaaacaga tacaagggcg
    13981 acccgctttg tgattaacga cgaccttcag caatagcggt tacggcttgt aattcagcgg
    14041 attcctgctc ttgctgttcc tggcgttcgc gaacgtcgag gatctggttg ttgatcagac
    14101 cttcttcgat ttcgcgcagc gcgattacag tggttttatc gttttcttcc ggtaccagcg
    14161 gatcctttcc gcctacctgc atctgacgag cgcgacgcgc ggcgaccagt accaggtcaa
    14221 aacggttacc aattttctct acagcgtcct gaacagttac gcgtgccata cttaaaaagc
    14281 tccacaggtg aagaaatgac tgggcatgat actgaaatca ggttcagtct gccaacaatt
    14341 tgctgattaa agcgtcatga cgctgctttt ggcggctcat gcgcagacgt tcggcgcgaa
    14401 taatggtctt caaatcggtc aacgcggtat cgaagtcatc attcacaatc agataatcat
    14461 attcggcgta atggctcatt tctgcaacag cttgcgccat acgctttgca atgacctctt
    14521 cgctgtcctg accgcgaccg cgtagacggc ggtccagttc aattttggac ggcggtaaaa
    14581 taaagatact ccgcgcgtgc ggcatcttct ggcgaatttg ctgcgcgccc tgccagtcga
    14641 tatcgagaaa aacatcgaca ccggtcgcca gtacttgctc aatggcctca cgcgaagtgc
    14701 catagtaatt accaaaaact tctgcgtgtt cgaggaacgc atctctgcta atcatttctt
    14761 taaattcatc atgattaaca aagaaataat gttcaccgtg gacttcacca ggacgcggtt
    14821 ggcgtgtggt gtgtgaaaca gaaacctggg tgtcatacaa cggttgggtt tttaataaag
    14881 cctgaatcag gctggattta cccgcgccac tgggggcaga aacaatataa agcgtgcctt
    14941 gagccatgag tatctttcgt atgtgattag cgaaataaag cctacatacg agcttattat
    15001 acacggcgcg gccatgtgac gtagcccttg ctcacacttt ttgcgcaaag ttgttgcgtt
    15061 ttgcgtagct gtttccgcat ttgcctgtga tttactgcaa taaagacgat tctcaggaag
    15121 cgtcctcgca gactccgttt ttgccactcg gctttgcctt caccacgttg tatatctgcc
    15181 agcctgacta cagcggaggt gaagatgaaa gtatggatgg cgatattaat aagtatcttg
    15241 tgctggcaat catctgtgtg ggcggtctgt ccggcctggt cgccagccag agcacaggaa
    15301 gaaatttccc gcctgcaaca gcaaataaaa cagtgggacg atgactactg gaaggaagga
    15361 aaaagtgagg tggaagacgg tgtttacgat cagttaagcg cccgtcttac gcagtggcaa
    15421 cgctgctttg tgagcgagcc ccgcgacgtg atgatgccgc ctttaaatgg tgcggttatg
    15481 catccggttg cccatacggg tgtacgcaaa atggcggata aaaacgcatt aagtctgtgg
    15541 atgcgagaac gtagcgatct ttgggtgcag ccaaaagttg atggcgttgc ggtaaccctg
    15601 gtttatcggg acgggaaact gaacaaagca atcagtcgcg gtaatggcct gaaaggcgag
    15661 gactggacgc agaaagttag cttaatttcc gctgtgccgc aaaccgttag cgggccttta
    15721 gccaacagta cgcttcaggg ggaaatattt ctccagcgcg aggggcatat ccaacaacaa
    15781 atggggggaa taaatgcccg cgcaaaagtt gctggcctga tgatgcgcca ggggaatagc
    15841 gacacgctaa actcactggc tgtttttgtc tgggcatggc cggatggacc gcagttaatg
    15901 accgatcgtt taaaggagct ggctaccgca ggttttactc taacgcagag gtatacccgt
    15961 gcggttaaaa atgctgatga ggttgcgcgc gtacgcaatg agtggtggaa agcgaaatta
    16021 cccttcgtca ccgatggcgt ggttgtgcga ggggctaagg aacccgaatc ccgtcattgg
    16081 ctaccgggcc aggcggagtg gctggtggcc tggaaatatc aacctgtagc tcaggttgcc
    16141 gaagtgaagg cgattcaatt tgcggtgggt aagagcggta aaatatcggt ggttgcgtca
    16201 ctcgcacctg tcatgctgga tgataaaaaa gtccagcggg tgaatattgg ttccgtcagg
    16261 cgctggcagg agtgggatat tgcgcctggt gatcagatcc tcgtcagcct tgccggtcag
    16321 ggtattcctc gcattgatga tgtggtctgg cgcggtgcag aacgtacaaa accgacacca
    16381 ccagaaaacc gctttaactc gttgacctgc tactttgctt ctgatgtttg tcaggaacag
    16441 ttcatttcac gcttagtctg gctggggtca aaacaggttc tggggctgga tggcattggt
    16501 gaggccggtt ggcgcgcgct gcatcagact catcgctttg agcatatctt ttcctggctt
    16561 ttattaacgc cagagcaatt acagaacacg ccggggatcg cgaaaagtaa aagtgcgcag
    16621 ttatggcatc agtttaatct ggctcgtaac cagcctttta ctcgctgggt gatggcaatg
    16681 ggaataccgc taacccgggc ggcgcttaat gccagtgatg aacggtcctg gtcgcaactt
    16741 ttatttagca cggagcagtt ctggcagcag ctgccgggga ctggatctgg gcgcgccaga
    16801 caggttattg aatggaagga aaatgcgcaa atcaagaagc tgggcagttg gctggctgcc
    16861 cagcagatca caggttttga accttagtga ccttcgtggc tgtagtaaaa gaccggtaat
    16921 cccagcttaa gacgtagcgc cagtaaacga gcaaaaaagc cgaatactag ggtggagata
    16981 atcaccacat catggttaga aacatagtgt tgcagcgcga tgtacagcac ggcggaggca
    17041 aatgagacac cggcatacaa ctctttttga aagaccagcg gaatgcgttt acagaacata
    17101 tcgcgcaaaa cgccgccaaa tacaccggtg gttaccgccg cgacaacggc aataattggg
    17161 ccgtgcccca tatccagtgc aacctgtgcg ccgatgatag aaaagaccac cagtccgagc
    17221 gcatcgagca ccagaaacac tttgcgcagg taaggcatta caggagcaac gatagtggtg
    17281 agtactgcgg cggtagcgac gataatcaca tattccgggt gtttgaccca gccgagcgga
    17341 tagtggccca gcagaatatc gcgcactgac cctccgccaa ttgcggtggc ggttgcaata
    17401 ataattacgc caaatgtatc catgcgccgt cgcccggccg ccagcgcccc cgtcatggct
    17461 tcggcagtaa tgccaaccaa atacaaaatg tgtaacagca tggttctctc cctaaaaatc
    17521 tgtctggcag ggtagcgatt tgtgcgcatt gtcacgattg atatttttta aggtgagagg
    17581 aaattgataa cttttagtta tgtgaaatcg ctattttctg tagcgtagat gcattcttct
    17641 gacttcttgt ctgagaaaat gcataagaaa aaataggtta aaactgaggg gggaaaagaa
    17701 aggtgcaatg tttagccatt gtgttcactg cacctggacc ttggcttacg catcgggtgt
    17761 ctcaatcaac aaaaagagtt tttcgtttct gctatttctt ctctgtaatt tttacactgt
    17821 tttccaactg cttgatgctg acctgcgggg tcggcaactc ctcaggcatg gttccgccaa
    17881 gttcctgaat ggtttgcctc accttgctac ccacgtcaaa gtgtgtggta ttagcttgct
    17941 gttttgaatt cacgctatcc cgcttgagtt tttcttctgt ttgggtagct cgaaagagat
    18001 tagccgccag ttctgttgaa cccatatgat ccaggatctt ctgactcttt ttcagctcct
    18061 tcagttggtg gatggctttc tgatctaatc caccatacag cccctggtaa ccatgattct
    18121 gaaatatggc gaaatcagta gctgtcgcta ccccagcttg ctgtgctgcc tcaaccaatt
    18181 gtttgttatg ttctttcaac tcatttctca gaaacagacg cttttcgtct tcacgaagtt
    18241 gcttaaatgc ttcatcgtca gcaagctcct gccgtcgggt ctggatagta aaataagtct
    18301 gccctgccgc aatgaccggt ttcgcggggt cgccgttttg tactaccagg taacaggcat
    18361 agcgggagag tacgatgtct tttaatgctc ttttggcatt tgagccaatt ttgaccatat
    18421 cgaggatctc ctcgaaatgg tcatcaattg tgtgaccact gttctcacag gcctctttgg
    18481 cgcgcgttaa tacaggtata aagtgacgat actctgaata acccagtagt ttcccaagtt
    18541 gtcttgctga ccattgttct gctc
//
LOCUS       DADLSA010000076        18528 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.076, whole genome shotgun sequence.
ACCESSION   DADLSA010000076 DADLSA010000000
VERSION     DADLSA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.076"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..419)
                     /locus_tag="JN691_002530"
     CDS             complement(<1..419)
                     /locus_tag="JN691_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7243946.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLG"
     gene            526..822
                     /locus_tag="JN691_002531"
     CDS             526..822
                     /locus_tag="JN691_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7243947.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(956..1381)
                     /gene="arsC"
                     /locus_tag="JN691_002532"
     CDS             complement(956..1381)
                     /gene="arsC"
                     /locus_tag="JN691_002532"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAY7243948.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1394..2683)
                     /gene="arsB"
                     /locus_tag="JN691_002533"
     CDS             complement(1394..2683)
                     /gene="arsB"
                     /locus_tag="JN691_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HAY7243949.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLSMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2737..3090)
                     /gene="arsR"
                     /locus_tag="JN691_002534"
     CDS             complement(2737..3090)
                     /gene="arsR"
                     /locus_tag="JN691_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HAY7243950.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            3833..3916
                     /locus_tag="JN691_002535"
     CDS             3833..3916
                     /locus_tag="JN691_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAY7243951.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3970..5322)
                     /gene="gorA"
                     /locus_tag="JN691_002536"
     CDS             complement(3970..5322)
                     /gene="gorA"
                     /locus_tag="JN691_002536"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAY7243952.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5394..6236)
                     /locus_tag="JN691_002537"
     CDS             complement(5394..6236)
                     /locus_tag="JN691_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAY7243953.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6439..8481
                     /gene="prlC"
                     /locus_tag="JN691_002538"
     CDS             6439..8481
                     /gene="prlC"
                     /locus_tag="JN691_002538"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAY7243954.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8489..9241
                     /gene="rsmJ"
                     /locus_tag="JN691_002539"
     CDS             8489..9241
                     /gene="rsmJ"
                     /locus_tag="JN691_002539"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAY7243955.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9290..10759)
                     /gene="dtpB"
                     /locus_tag="JN691_002540"
     CDS             complement(9290..10759)
                     /gene="dtpB"
                     /locus_tag="JN691_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HAY7243956.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(11077..11511)
                     /gene="uspA"
                     /locus_tag="JN691_002541"
     CDS             complement(11077..11511)
                     /gene="uspA"
                     /locus_tag="JN691_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAY7243957.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11902..12237
                     /gene="uspB"
                     /locus_tag="JN691_002542"
     CDS             11902..12237
                     /gene="uspB"
                     /locus_tag="JN691_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAY7243958.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12308..13807)
                     /gene="pitA"
                     /locus_tag="JN691_002543"
     CDS             complement(12308..13807)
                     /gene="pitA"
                     /locus_tag="JN691_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAY7243959.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14039..15238
                     /locus_tag="JN691_002544"
     CDS             14039..15238
                     /locus_tag="JN691_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY7243960.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKIPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSQKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
     gene            16404..17915
                     /locus_tag="JN691_002545"
     CDS             16404..17915
                     /locus_tag="JN691_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HAY7243961.1"
                     /translation="MPLQQFSRLALTLAIGLSIAAGGAALTWYQVAETDLSRTAAAAH
                     EAVSVTERLLDEAQGAIDLVYPLSVQPCTPQLRTELGRLTISHEHIRVINIFNQNLLS
                     CSSWEGADPVREFISAGDNQTLILATDDYISPGVPVMILRKTDNDNAITASIATKWVA
                     DNMTLLNLRRPLSLRVGNTVLTSDNTLVKAIPGGNRHAVYSGLYPFSIEYPGDLSVPL
                     TLYFHDGALSLLLSVLLGAAVATGLWKIKFRRKSRYDEIYEAMRKDEIVPWYQPIMDA
                     STGEIAGVEVLARWVKADGEVIYPGSFITEVERSDLVIPLTRSLMARASRDLLPLTGD
                     GKRWHISFNITQSHIMETGFIAECLTFIDAFTPGSITLTIELTEREPFDNSDEMRCQL
                     KRIHDAGIALALDDFGTGYANMEYLSEIPVDIIKIDRVFVNRIGQGESAEHLLTSLIE
                     MANTLKKEIIAEGVETEAQAAWLYERGVGKLQGFLYSPALPPDKLALFKPKMS"
BASE COUNT         4680 a         4576 c         4786 g         4486 t
ORIGIN      
        1 cccagagaat ctaggataat actggcaaga acggagacgc cggctgcgat aatcgcgtac
       61 aacaaggtga ctttccaacc aaaggtcatc cacattagtc cgacgataat gggattaagt
      121 aatggtgaaa caaataaaaa agtgagagtc ggaccaaacc cggctttcgc tgacaacaat
      181 ccacgtaaca tgggtattgt cgagcaacta cagaacgggg taacggctcc caacagagca
      241 gccaggagat agcctctccc ttttctggcc cccatcatct gctggatttt atggtctggc
      301 accttttgtc ttatcaggct gacaccggca ctaatcacta taaacagcaa cgaaagctcg
      361 acggcgagaa acacaaacat ctctgcggca tcttgcagca tcgcaagcca actactcata
      421 cgtaacctca ttggataaat ctatatttcc agaataatcg aaatgaaatt cagatcaaga
      481 tatttctggt attatcgaaa tatcaaccag acagaggggt gtcatatgca gttggaagaa
      541 gtcgctaaag cgctgaaaga gctaggtcac ccgacccgtt tgttcatatt taaacatctt
      601 gtcaaagccg gggaacaagg gttgccggtc ggtgagttgc aaaagcagct tgggatacct
      661 ggctccacac tcagccacca tatatcagca ctggtatcgg taggactggt caaacaaaac
      721 cgtgagagtc ggacgctgat gtgtgtttcg caatacgcaa tgctggaagc gataattgaa
      781 ttcttacgcg aggagtgctg cgtaaatagc aaaattgcct gatgcgctac gctctacata
      841 tttcatgcca tatattgaat ttgcacggtt ttgtaggtcg gataagacgc gttagcgtcg
      901 catccgacat aaacaaagcg cactttacca actatcggca gtggatatcg ccgctttatt
      961 tcaggcgctt acccgcttca tcaaccactt tctcgccatc ttccttggag aatgcgcctt
     1021 tttgcgcatc tggcagaatt tccagcacca cttctgaagg gcggcacagg cgagttccca
     1081 gcggcgtcac cacaatcggg cgattaatca gaatcgggtg ctgaagcata aagtcgatta
     1141 accgatcgtc agtaaattta tcttccgcaa ggcccagctc ctcatacggt tcgacgtttt
     1201 tacgcagcag cgcgcgtacg gaaatcccca tatcggcaat gagtttgacc agttcatcgc
     1261 gcgttggcgg agtttccaga taatggataa tggtcggttc tgtgccgctg ttgcggatca
     1321 tctccagcgt attacgcgac gtgccgcagg ccgggttgtg ataaatggta atgttgctca
     1381 tatcagtatc tcattacaaa gtgaaagaga gacgtagcgc cagcgcagcc agcgtcacaa
     1441 acagcacagg cagggtcatg ataatccctg tacggaagta atatccccag ctgatagtca
     1501 tattcttctg cgaaagtacg tgcagccaga gcagcgttgc caggctacca atcggggtaa
     1561 ttttcggccc caaatcgcag ccaatcacgt tggcataaac catcgcttct ttgataacgc
     1621 cagatgccgt gctgccatca atggacaacg cgccaaccag taccgtcggc atattgttca
     1681 taatagaaga gaggaaggcg gtgaggaatc cggtgccgag cgtcgcggcc cacaggccgt
     1741 tatccgccag cacgttgagt acgccagaaa gatattccgt taatccggca ttgcgcaggc
     1801 cataaaccac cagatacatg ctgagcgaga agatgacaat ctgccagggg gcaccgcgca
     1861 ggactttacc cgtattaatc gcatgaccgc gtttagcgac gacaaataat atcagtgcgc
     1921 ccacagctgc aatggcgctc accggaatgc cgagcggttc caagacgaaa aatcccacca
     1981 gcagaagcag taaaacaacc cagccagttt tgaacgtagc aggatctttg atcgcttctg
     2041 cgggagattt cagcagcgcc atatcgtagt tctgcggaat atctttgcga aaatagagat
     2101 gtagcatcac cagcgtggca acaatcgcgg cgatatccac cggcaccatc accgaggcgt
     2161 attcgcgaaa gccgaggcca aagaaatcag cggaaacgat attcaccagg ttggagacaa
     2221 taagcggcag gctggcggta tcggcaatga atccggccgc catcacgaac gccagcgtcg
     2281 tgcctttact gaatcctaaa gccagcagca tggcgatgac aatcggcgtc aaaataagcg
     2341 ccgcgccatc attggcaaac agggccgcaa cggcggcacc gagcaggaca atccaggtaa
     2401 acagcaagcg accgcgtcca ttgccccagc gtgaaacgtg cagcgctgcc cattcaaaaa
     2461 agccggactc atccagcagc aggctgatga taatgacggc aataaacgcc gccgtcgcgt
     2521 tccagacgat attccacacc accggaatat cacccggatg aaccacgccc gtaaccaacg
     2581 ccagtaccgc gccgagcgtt gcactccagc cgatgcctaa acctttcggc tgccagataa
     2641 ccaatacgat ggtcaggaca aagatagcgc ctgccagtaa cataatgcct cccggataaa
     2701 acacatgtgc aaatccatat gtgtttagct aaatttttaa ctgcaaatgt tcttactgtc
     2761 tccggaacag ttttgtctgg ccaggttgcg gacaattgcc tgaatctttt cctgttcaca
     2821 tcgccaggct tcatcaataa ttttcgccgc ccatgctgga atatgcggcg ataagcggta
     2881 atgaacccac ttaccttgct tgcggtccag caatagcccg ctttcacgca gcaatgccag
     2941 atggcgggat atcttgggct gcgactgatc gagggcagtg cagagatcgc agacgcataa
     3001 ctctcccagt tcgctaagca gtaaaacgat gcccagacgg gtttcatcag caagaatttt
     3061 gaacaattgg atgggtaaca gaaatgacat attgcgctcc tgattgttgc aggtagtgtc
     3121 tctcttcgaa gcggataagt caaaaacata tatgacttaa cgaatgtgta agtgcagagg
     3181 aaggtaatag gtgtgaattt tgagttggct attcatttga aaggcggttt gaatcaggac
     3241 gtaaaaaaac cgactttgcg tcggtttttt actcttcagc ctgagttggt ggctctggct
     3301 ggagtgagaa tgcccacatc taacggttca atactattag ccagtggcta acattgcaag
     3361 cgttagcgat ttcttcagct ggagaaggga cagtttgcta tacatggtgg tgaaagagga
     3421 tttatatact ttcatcgggt cgctgccgta ccaagccttg tccgaatccg gacgtaaaaa
     3481 aaccgacttc gcgtcggttt ttttactctt cagcccagag ttggtggctc tggctggagt
     3541 gagaatgcca tcatctaacg catcaatgct aataccatac tgaaactatt gcaaggttat
     3601 gctggttatt tagcctaatt acactgtatg attatccagt tagctctgag gcattttcac
     3661 tctggcaatg cgcataaacg ctttcaaagt cctggtcaga agtacgggtg gtgccgttaa
     3721 ctgatgctct ggccggagtg agattgccca catctaacaa tgaggcatgc gccgtgacag
     3781 gcagtggatg agtaagcgga tgcattctca ctccatcgca tggagaaaac gggtgattga
     3841 taaagcaatc atcgttcttg gggcgttaat tgcgctgctg gaactgatcc gctttctgct
     3901 tcagcttctg aactgatagc ggaaacgtaa ttaagggcta agagcacact actcttagcc
     3961 ctttaacatt taacgcatgg tcacgaactc ttctgccgcc gttgggtgaa tggcgacggt
     4021 attgtcgaag tcttttttgg ttgcgcccat cttcagcgcc accgcgaagc cctgcaacat
     4081 ttcgtccata ccaaagccaa tgccgtgaat accgacaatc ttctcttctg gcccaacgca
     4141 caccagcttc atgcggcacg gctggcggtg agtggtgacg gcggtataca tcgcggtgaa
     4201 agaggattta tacactttca cctgatcgtc gccgtactgc tcgcgcgcct gcggttccgt
     4261 taaaccaaca gtaccaatcg gcggatggct gaagaccacg gtcggaatgt tgctgtaatc
     4321 cagatgctca tccggcttgt tattaaacag gcgttcagag agacggcgac ccgctgcaac
     4381 tgccaccggt gtcagttcca ctgcacccgt gttatcgccc accgcgtaaa taccttcaat
     4441 attggtgttt tgatatttat cgacgacgat atagcctttt tcgttggttt taacgccagc
     4501 ggcttcgagg ttgatgttgt cattggcagg ctcgcgacca atcgcccaaa tcaggcaatc
     4561 caccgtttca ctgcgaccat cttccagctc cagcgtcagg ctaccatcgg catttttcac
     4621 taccgctttc gggatggcgt tggtatgcaa ctgtgggcct tcggcgttca tcacttcgac
     4681 cagcgtttca gagatcatcg ggtcgaagct gcgcagcggc gcatgtttac gcacaaacag
     4741 atgcgttttc gcgccgaggc cgttaatcac gccagccagc tcaacggcga tgtaacccgc
     4801 gccaacaacc gccacgcgct ctggcaaagc aggaagggcg aagaagccat cggaatcaat
     4861 accgtattcc acgcctggaa tatccgggtg gctcggacga ccgccggtgg cgatcagaat
     4921 atgatcggcc gtgatggttt cgccgttaac ttccagcgtt ttggcatcaa cgaagcgggc
     4981 aaagcctttg attacatcaa cgttattttt accgagcacg ttttcatagg aagtatgaat
     5041 acggtcgata taggcggtac ggctggcgat caacgtttcc cagttgaact tgttgatggt
     5101 ggtatcgaaa ccataatcag ggccgtacat atggatcgct tcacggattt gcgccgcgtg
     5161 ccacatcact tttttcggca cacagccgac gttcacgcag gtgccgccca gctctttggc
     5221 ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg ttgatggagg cgataccgcc
     5281 gctgccgccg ccgatggcga tgtaatcata gtgtttagtc atgacatatt gtccttatcg
     5341 ttgattaccg cgattgtagc gcgagacgta ataggtgcca gcaatggctg caattactcc
     5401 ggcacgatcc agcttacggt ggcgtgcccg gtacctgccg gaaccagttt gctgtgcagc
     5461 cacgacagca cgttattcat ctgctgttcc agcttccacg gcgggttaat cacaatcatg
     5521 ccggaagcgg tcatgccacg gcgatcgctg tctggcagca ccgccagttc aatttgcaga
     5581 attttgcgaa tgccggtcgc ttccaggtcg tggatcatgc gcttaatttg ctggcgcagc
     5641 accaccggat accacaatgc gtaagtgcca gtggcgaaac gtttgtaacc ttctgctatc
     5701 ccgctgacca ccgcctgata gtcggttttc atttcatacg gcgggtcgat aaggattaaa
     5761 ccacggcgag aaaccggcgg cagtttggcc ttaagttgct ggaaaccgtc ggctttttcg
     5821 acgcgtgcac ggctgtcttt ctgaaatcca gaacgcagca acgggtaatc gctcgggtgc
     5881 agttcggtca gttgcaggct gtcttgttcg cgcagtagct ggcgagcaat caacggcgaa
     5941 ccggggtagt aacgcaactg gccgctacgg ttgaagtgtt ttaccacatt gatgtacgcc
     6001 tccagttctg cgggcaaatc gtcctgctgc cagatacggg cgataccttc gagatattcg
     6061 ccggtacgct cggcatgttc gctacctaac tgataacgcc cggcccctgc gtgggtgtcg
     6121 agatagagaa acggtttatc tttctctttc agcgactcga tgatcaggct ctgaacggta
     6181 tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt ggcgataact gagcatgggt
     6241 aaaggtgttc cggtaagtaa aaattggccc gtattcgggg cgcacaaaag cctatcagga
     6301 cagtataccg aaaagaggcc gccgccgcga aagcgtaacg tttctcattg aaattcacta
     6361 cacttaaccc catgctacat acattatgta aagcgcctgt tgagcgcttc cttaacctct
     6421 ttaaccagga ctgcgctaat gacgaatccg ttactgactc cctttgaatt gcctccgttt
     6481 tctaaaattc tcccggaaca tgtcgttcca gccgtgacta aggcattgaa cgactgccgc
     6541 gaaaatgtgg agcgcgtagt agcgcaaggg gcaccgtaca cctgggaaaa tctctgccag
     6601 ccgttggcgg aagtggacga tgttttgggg cgtatcttct ccccggtcag ccacctgaac
     6661 tcggtgaaaa atagcccgga actgcgtgaa gcgtacgaac aaaccctgcc gctgctgtcg
     6721 gaatacagca cctgggtagg gcaacatgaa gggctgtata aagcgtaccg cgacctgcgc
     6781 gatggcgatc attacgccac gctgaacacg gcgcagaaga aagcggttga taacgccctg
     6841 cgtgattttg aactctctgg cataggtctg ccgaaagaga aacagcagcg ttacggcgaa
     6901 attgctaccc gtctttctga actgggcaac cagtacagca acaacgtcct cgatgcgacg
     6961 atgggctgga ccaaactcgt taccgacgaa gcggggctgg cggggatgcc agaaagcgcg
     7021 ctggctgcgg caaaagccca ggccgaagcg aaagagctgg aaggctacct gctgacgctg
     7081 gatatcccaa gctacctgcc ggtaatgacc tactgcgaca accaggctct gcgcgaagag
     7141 atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc cgaacgccgg taagtgggat
     7201 aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg aactggcgca actgctgggc
     7261 tttgaaaact acgcctttaa atcccttgcc actaaaatgg cagaaaaccc gcagcaggtg
     7321 ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc aaggcgaaaa agagctggag
     7381 caactgcgtg ccttcgctaa agccgaattt ggtgtcgatg agttgcagcc gtgggatatc
     7441 gcttactaca gcgaaaaaca gaaacagcac ctctacagca tcagcgacga gcagctgcgt
     7501 ccgtacttcc cggaaaacaa agcggttaac ggcctgtttg aagtggtgaa acgtatttac
     7561 ggcatcaccg ctaaagagcg taaagatgtt gatgtctggc atccggatgt acgtttcttc
     7621 gaactgtatg acgaaaataa cgaactgcgc ggcagcttct acctcgatct gtatgcccgt
     7681 gaaaacaagc gcggcggggc gtggatggat ggctgcgtag gccagatgcg taaagctgat
     7741 ggttctctgc aaaaaccggt cgcgtatttg acttgtaact tcaaccgccc ggtaaatggt
     7801 aaaccggcgc tgttcactca cgacgaagtg atcaccctgt tccacgagtt cggtcacggc
     7861 ctgcaccata tgctgacccg catcgaaacc gctggtgttt ccggtatcag cggtgtgccg
     7921 tgggatgcgg tcgaactgcc gagtcagttt atggaaaact ggtgctggga gccggaggcg
     7981 ctggcgttta tctctggtca ctatgaaacc ggcgaaccgc tgccgaaaga gttgctggat
     8041 aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta ttctgcgtca gctggagttc
     8101 ggcctgtttg atttccgcct tcatgccgag ttccgcccgg atcagggggc aaaaatcctc
     8161 gaaactctgg cagaaatcaa gaaactggtt gccgtggtgc catctccgtc ctggggccgt
     8221 ttcccgcacg ctttcagcca tattttcgcc ggtggttatg cggcaggtta ctacagctac
     8281 ctgtgggctg acgtactggc ggcagatgct ttctcgcgct ttgaggaaga gggcattttc
     8341 aaccgtgaaa ccgggcagtc gttcctcgac aacattctga gccgtggcgg ttcagaagag
     8401 ccgatggatc tgttcaaacg cttccgtggt cgtgaaccgc agctggatgc gatgctggag
     8461 cattacggca ttaagggctg atcattcagt gaaaatctgc ttaattgatg aaacaggcgc
     8521 cggagacggc gccttatctg ttctggcggc ccgctggggg ctggagcacg atgaagacaa
     8581 cctgatggcg ctggtgttaa cgccggaaca tctggaattg cgcaagcgtg atgagccaaa
     8641 acttggcggc atctttgttg attttgttgg cggagcgatg gcgcaccgac gcaaattcgg
     8701 cggtggtcgc ggtgaggcgg tggcgaaagc ggtgggcatt aaaggcgatt atttgccgga
     8761 tgtggtggac gccaccgcag ggctggggcg cgatgccttt gtactggctt cagtcggctg
     8821 ccgcgtgcgg atgctggagc gtaatccagt ggttgccgcg ctactcgacg acggcctggc
     8881 gcgtggttat gcggatgcgg aaatcggcgg ctggttgcag gagcggttgc agttaattca
     8941 cgcctccagc ctgacggcgc taaccgatat taccccacgc ccgcaggtgg tctatctcga
     9001 tccgatgttc ccgcataagc agaaaagcgc gctggtgaaa aaagagatgc gtgtctttca
     9061 gtcgctggtg gggccggatc ttgatgccga tggattactg gagcctgcac gcttgctggc
     9121 gaccaaacgc gtggtggtaa agcgcccgga ctacgcgcca ccgctggcga atgtcgccac
     9181 gccaaacgcg gttgtgacta aagggcatcg ctttgatatc tatgcaggta cgccagtgta
     9241 aaagtaaagc agggtagcga taacgctacc ctgctttctg caagaataat taatggcttt
     9301 ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa cagcatcact actgcaacgc
     9361 ccagcgtgac cagaccaatc ttaccaaaca cgttggtata gacgggcaac gtctcaagcg
     9421 gatcggtaat gttgtccggc accgcggtaa atgttgccac atagccgccc agcaagaacg
     9481 cggcagcctg cgtcaggaac cacatcccga gaataaagcc catcaaatgc tgcggcacca
     9541 gggcagcaat catcgccagg ccaagggcgc taataaacaa ttcacccagg ctctggaata
     9601 aatacaccag cacgataaac catggcgatg tcagcccttg ggcatccgca aaccacattc
     9661 ccgcagcagc agccgtcaaa aagcccagtg agcacataaa catgccgaga gtaaatttca
     9721 tcggcatcga gaggtctttg cctttgttac ccagatgcgt gtaaatgcct gccagtattg
     9781 ggctggcgag taccacccag aacgggttaa gcgcctggaa gctgaccggg ttgatggaaa
     9841 aaccgagaat ttcatgatgc acgttgttga tggcaaagaa gttcagcgat gttggcatct
     9901 gggcgtagag aatgtaaaac accaccgctt cgagcatcag gacaaaggcg acaaacattt
     9961 tattgcgccc ggttttatcc agcttgaatg cctgacgaaa gaagatgatg gtgacgacga
    10021 tggagagaac aatcagcacc agattggcga cttctacgtt gtgcatcagc catgcacata
    10081 cgaagatcat caccacgctg ccaagtaata cgtacaacag tttgctgaag ctcatcggct
    10141 tgaagtcggg ttcagaacca atgtctttca ccattccacg acaggcgatg taaaccagta
    10201 atgcgataat taaccccgcc ccgcacaggt tgtaggtgac tgaataaccg aatctatcag
    10261 cgatcacagg ggccagcgat aacgctatca acgagccgat attgatcgac atatagaaca
    10321 gggtgaatgc gccatcaagc cgcggatctt tcggcggata gcacttcgaa agcaagctgg
    10381 ctgggttagc tttaaacagg ccgttaccga cagcgatagt ccccagggcg atgaaaatca
    10441 ggtcaggctt aagtagcgac atgccggtca tgaagtagcc aatcgccagc acaagtgctc
    10501 caagaacaat ggtgcgtttg gtccccaaca ggtggtcgcc gacatagccg ccaatggaaa
    10561 tgaggccata gaccagtgca gcaaaagcac caaaagtgac aaaagcctgc tcttgcgaga
    10621 atccaagctg tttaacgaag aaaaccgcca gtacgccctg cacgccgtag tagccgaatc
    10681 gctcccataa ctcgacaaaa aagatcatga aaaatgggcg aggttgctgc agcatcccca
    10741 tgggtgttgt tgtattaata tttattaacc ttccaatacc atcctgaaaa gtatgaacgc
    10801 gtaagggata acgcgcgaaa aagtttacat ctgtcagaga ttcttaatat tgatgcggtg
    10861 aatagattaa tacactatta caacagaaaa taaccagctg atcacactaa tgatgggaaa
    10921 tatgatggtg ttaaattttg tattggcagg agattttgat tgcgtacgca gatcgtaggc
    10981 cagataaggg gtttacgctg catcgggtaa caaggcgcgc caaaaagcaa aacgcccgca
    11041 tatgcgggcg ttcaggaaca cgtcgtggag ggatgattat tcttcttcgt cgcgcagcgg
    11101 aacaatcagc atatcaacgt gaacggtgtt gatcagctga cgtgcggaag acatcagttt
    11161 gctccagaag tcctggtggt gaccacaaac caccagatcc atatcgtatt tcttgattgc
    11221 atcgaccaga acctggccca ggtcgccgct gccgctcagg gtttcagtga ttggatagcc
    11281 tgcattagtg gaaagctcgg tcagcgcatg atgtgtctct tcagagatgc gtttctgcat
    11341 atcacccaga ttcacatcaa taagcccggt gtataggtca gagtagttta catctacgtg
    11401 gatcagagaa actttcgcat tgtaggggcg agccatagag actgctttct ctaccagaac
    11461 tttgctttcc ggggagagat cgactgcgat gagaatgtgt ttataagcca tagtgttact
    11521 ccttccataa agttgtcgat gactggccag ctagcgtttc ttgtgccgtt acgtcacccg
    11581 cgtcctgcga actacgtgcg cggggctaac ccagccgtaa agattcagtg ttaagaccat
    11641 ccttacctta tagcgacccg gatgatccgt caatccgcct tgcttacccg ttagataaac
    11701 aaattttcga catgaatata ttgatagtgg ttaaccttct ggaaaaaaaa caacctgatc
    11761 tcctacacta tctatagagc cgctcggatg ttggtcacca caaaagcggt ttctccagga
    11821 cctggtgtta actgattggc agcgtatcgg gagcggtagc cccggggtgg aactccgcgg
    11881 gcaggtcgcc ggggaggagg tatgataagc accgtcgcat tattttgggc tttatgtgtc
    11941 gtttgcattg ttaacatggc gcgctatttc tcatcactac gcgcgttgtt agtggtactg
    12001 cgtaactgcg atccattgct ctatcaatat gttgatggag ggggcttttt tacctcacat
    12061 ggccaaccca acaaacaggt gcgtctcgtt tggtatatct atgcccaacg ttatcgcgat
    12121 catcacgatg atgagtttat tcgccgctgt gagcgggtgc gtcggcagtt tattctgact
    12181 agcgcattgt gtggtctggt ggtggtcagc ctgattgcat tgatgatttg gcattaagca
    12241 cttcagcaat aataacgcat aaaaaaagcg ggccagtaaa ctgacccgct cgttttggtg
    12301 cgtacgatta caggaactgc aaggagagcc agtacagccc gccggaaagt agtaccgcag
    12361 ccggaagggt aaacacccag gccatcagaa tactggttac ggttttacgc tgtaagccgc
    12421 cgccatctac caccatcgtc cccgcgacag aagaggagag tacgtgagtg gtggaaaccg
    12481 gcatcccggt ataactcgcc aggccgattg acactgccgc cgtcatctgg gcagacatcc
    12541 cctgagcgta ggtcatgcct ttcttaccga ttttctcacc gatagtcgtt gccacacgac
    12601 gccagccaat catcgtaccg atacctaacg ccagcgcgac cgccatgatg atccacaccg
    12661 gcgcatactc gatggtgcta agcatgtcgg acttcagttt cttcaacagg cgctgatcgt
    12721 cagcactcac accaggcatc ttcaccactt tgtcgatagt gtcagaaacg cacagcataa
    12781 tgcggcgcat ctggctacgt tgatcaagcg acagcttgtc gtagctttcc acatcggtgg
    12841 tcaacatacc tttcaggcgg ttgagcgcgt taatggtatt cgacggatgg cagtggaact
    12901 ccgcaggttg cgttgcgcca gcttccggag ccggtactaa ctgatcagca ccggtagcct
    12961 gtttgagcag cgcaggatgc tgctcaaagt aagcttcgac gttgttgatg gcatcacggg
    13021 tacgggtgat ttcgtagcca gtggcattca tgttcaccac gaagcctgct ggcgcgacgc
    13081 caatcaatac caacataacc agaccaatgc ctttctgacc atcgttcgcg ccgtgcgaaa
    13141 acgccacgcc gatagcggaa aggatcagcg cgatacgcgt ccagaacggc ggctttttct
    13201 tgccgtcttt cttttcacgc tccgctgggg tcaggtggat acgggcgcgt ttcttggtgc
    13261 cgctccagta gcgacgcagc aagaaaatca gaccgccagc aaacaccagg ccgacaatag
    13321 gggaaacgat cagagaaccg aaaatactta atactttcgg gatattgagt gcatccacca
    13381 ctgacgtccc ggtcatcaac gcattggtta aaccaatccc gatgatcgcg ccaatcagcg
    13441 tatgagagct ggatgcaggt aaaccaaagt accaggtacc caggttccag ataatcgccg
    13501 ccagcaacat agagaacacc atggcaaggc catgagacga tcccatatta agcagcagat
    13561 ccgtcggcag catatgcaca atggcatagg caacactcag accacccagc aaaacaccca
    13621 aaaagttgaa caccgccgcc ataaccacgg cgagctgaga acgcatcgcg cgggtataga
    13681 taacggttgc cacggcgttg gctgtgtcat ggaaaccatt gatggcttcg tagaacagca
    13741 caaaagccag tgcaagcaat aataacagcc cggtatgcaa atccaggcca gcaaacaaat
    13801 gtagcatagg acgttacgcc attttgagga catgaacgcg gcgcattatc agtgactttc
    13861 gcggcgcggg caaagtgaaa tatagacttt ttttgatttg cctcctgtat ggatttcact
    13921 caaaaaataa ttatcttata taattcaggc aaatacttcc ttttagtaat attgatgctg
    13981 gtgcgaccac tgaggaatct ttacaattca cgcccgtttt ttctaagagg agcgcaacgt
    14041 ggaaaggttt gatgccatta ttataggcgc tggtgcggcg ggtatgttct gttctgcgct
    14101 ggcaggtcag gcaggacgcc gggttctgct gatcgataat ggtaaaatac cagggcgcaa
    14161 aatccttatg tctggcggtg ggcgctgcaa ctttaccaac ctttatgtcg aaccaggcgc
    14221 ttatctgagc cagaatccgc atttttgtaa gtctgcactc gcacgtttta cccagtggga
    14281 tttcattgat ctggtcaata aacacggtat cgcctggcac gagaaaacgt tagggcaact
    14341 cttctgcgat gactccgcgc agcagattgt cgacatgctg gtggatgagt gcgagaaggg
    14401 caatgtaacc ttcagattgc gtagcgaagt gctgagtgtg gcgaaggatg aaacaggctt
    14461 cacgcttgat ctgaacggca tgactgtcgg ttgcgaaaag ctggtcatcg cgaccggtgg
    14521 gctgtcaatg ccggggctgg gcgcatcgcc gtttggttat aagattgccg aacaatttgg
    14581 cctcaacgtg ctgccgaccc gcgcgggtct ggtgccattc actctgcata aaccgttgct
    14641 cgaagagtta caggtgctgg cgggcgtggc ggtgccttcc gtgattaccg ctgaaaacgg
    14701 caccgttttc cgtgagaact tactcttcac ccaccgcggc ttatctggac cggcggtgtt
    14761 gcagatttca agctactggc aaccgggtga atttgtcagt atcaatctgc taccggatgt
    14821 ggacctcgaa accttcctga atgagcagcg taacgcacat ccgaatcaaa gcctgaaaaa
    14881 cacactggcg gttcatctac cgaagcggtt ggttgaacgc ttacagcaac tcgggcaaat
    14941 cccggatgtt tcgcaaaaac agctcaacgt gtgtgaccaa caggcactga ttagcacatt
    15001 gaccgactgg cgcgtacaac ccaacggcac tgaaggctat cgcactgccg aagtgacgct
    15061 cggcggcgtg gacaccaacg aactttcttc acggacgatg gaagcgcgca aagtgcctgg
    15121 gctgtacttc atcggcgaag tgatggacgt taccggctgg ctggggggct ataacttcca
    15181 gtgggcgtgg tcgagtgcat gggcttgtgc gcaggatttg gcgtcaattg tgaaataacc
    15241 atgacgtttt cccgtcagcc gtttgtatat gtcgttatta agtcattttg gtacggttct
    15301 gcatccgaca ttctggtgtt aactcgctgt tttgttagcg aattaacgct attgccgttg
    15361 tggttatttt atagtgtatt tattctcaga gaatatatat tactgaaata aaaaacacaa
    15421 agaataacca aaggaaatta tttgaaacgt aaaaggtata tatttttaat ctgtaaaatt
    15481 taaaatcacc ccctctggat tgaatcaact tgctctattt aagacgattg tttttacatt
    15541 ttcaccacct gtatcaggtg gtgttttttt attggcggca atgcctttat cttattttta
    15601 ttacgttgta aatatggcta ttcctaaatg aaatttgatt tgctcagttt atttgtgatg
    15661 tcaggggagt gcttgccatg agagtcgtga tattattatt catcattttt ataccgagtg
    15721 tggcggatgc tttttcattc gatagttcaa taattaatct caaatgtcct aggcgtggaa
    15781 ctatcgaaat aatattacac agatacgaac acacccagga aagctgggga ggggagaatt
    15841 ttgaaaccgg gagtgggtat agtcatgagg gcgcgttatt tatttttgac tttgccaatc
    15901 aggataaaat gatttacaat cgcgcggaga attcgtttgc tttttggtat gaatcttccc
    15961 ggaaattagt taactgtaac ttaataacgc ttaaaagcac atatcctgtt aacctgcctt
    16021 atgttcatgg ctgatgtgat gtgtgtcggg aactaacatc ttcatctttg tatatgacat
    16081 tacgataatg ttaagctggt ctttgtcata aatttaccct tagagatact actcccttct
    16141 gtcaatatta ttcaagccga acgttatact ggcttttgtc ggtcacggat atcttttttt
    16201 cagtgagaat tcaaagtgag ggtatataag aagtcgtatg agtaagtatc tttgttgtgg
    16261 tgggttatta ttttatgggc aaaatattaa gcagcgaaat cttttttttg cccagacgtt
    16321 ttttgtagaa aaaagaattt attagtccgg ctgaaaagca agaactgcta taactaatta
    16381 agcgtaacgg aacggtacaa aacatgccct tacaacaatt ttcccgatta gcgctcactc
    16441 tggcgatcgg cctttcgatt gctgcgggtg gggccgcgct gacctggtat caggtcgctg
    16501 aaacagacct gagcagaaca gccgctgctg cccatgaggc ggtctcggtt actgaacgac
    16561 tgctggacga agcccagggc gcaatcgatc tggtttatcc cctgagtgtg caaccatgta
    16621 ccccacagct cagaaccgaa cttggccggt tgaccattag tcacgaacat atccgggtga
    16681 ttaacatttt taatcagaat cttttgtcct gctcgtcctg ggagggcgca gatcctgtgc
    16741 gggagttcat ctccgctggc gacaaccaga cgctgattct ggcgacagac gattatatat
    16801 cacctggtgt gccggtcatg attctacgca aaacggataa tgacaacgcc ataactgcga
    16861 gtatcgccac taaatgggtg gctgacaata tgacacttct gaatcttcgt aggccgctgt
    16921 cgctgcgcgt agggaatact gttcttacca gcgataacac gctggtgaaa gcgatacctg
    16981 gagggaatag acacgcagtt tattctggcc tgtatccctt ttccatagaa tatcctggcg
    17041 atctttccgt tcctctaacg ctttattttc atgatggtgc gttatcttta ctgctttctg
    17101 tgcttctggg ggccgctgtg gcaaccggcc tgtggaaaat aaaatttcgc cgtaagagcc
    17161 gctatgatga aatctacgag gcaatgagga aagatgagat tgtgccgtgg tatcagccaa
    17221 ttatggatgc ctcaacaggt gaaatagctg gcgttgaagt acttgctcgg tgggtaaaag
    17281 cagatgggga agtcatttat cccggcagtt ttatcactga agtagaacgt tcagatttag
    17341 ttattccatt gactcgtagc ctgatggcgc gggcatcccg ggatttactg cctctgacag
    17401 gcgacggaaa gcgttggcat attagtttca atattacgca gtcacacatt atggaaacgg
    17461 gatttattgc cgagtgcctg acgtttattg atgcttttac accgggcagt atcactctca
    17521 ctatagagct cacagagcga gaaccctttg ataacagcga tgagatgcga tgccagctta
    17581 agcgtattca tgatgcaggc attgcccttg cgctggacga ctttggcacc ggttacgcaa
    17641 atatggaata tcttagtgag atcccagtcg atatcatcaa aattgatcgc gtctttgtga
    17701 acagaatagg tcagggcgaa tctgcagaac acctgctgac cagccttatc gagatggcca
    17761 atacgctgaa gaaagagatt atcgccgaag gtgtggaaac cgaggcgcag gccgcgtggc
    17821 tatatgagcg tggtgttggc aagttacaag gattcctata ttcaccggca ttaccgcccg
    17881 ataagttggc actgtttaaa ccgaaaatgt cgtgagttca acggtacgcg ctatcggaat
    17941 cattgaaccg gtgagggccg ccccctttat tactcagggg gggctttcaa cgttctgaat
    18001 taagatgttt tatgactcac tgagcaggat gtgagttcca acgtaatctc cgctattttt
    18061 actgtttttc atttgctgcc tcatctatcc cttcgccttg cctctgagtt gcgttaaagg
    18121 ctgatgtact gcctttaacc cgttcaatac atagcctttt tgtcaatcat catcccgaag
    18181 agcgttgcac tgtgccttcc atagctgtgg ttgtcctctc aatctgtcag cccatttatg
    18241 gaaaatctta acgaagcaac aatgagatat gtaaaaaaag ggccgattta atcattcatt
    18301 aagacaggtc attttttctt actgtgaggg cggtaagctt ttctgtcggt aacaggttgg
    18361 gagatgctgg tattatgaga tttttctacc gtcctgcggc ggaatatgtg aaaaatagaa
    18421 atgattatta ttattgcatg gttattttta gtttgtgatg gtggagaata atcctattcc
    18481 cgtgaccttg ttttttacat tttatttaac tgcatggtat atgcattt
//
LOCUS       DADLSA010000077        18312 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.077, whole genome shotgun sequence.
ACCESSION   DADLSA010000077 DADLSA010000000
VERSION     DADLSA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.077"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            264..491
                     /gene="feoA"
                     /locus_tag="JN691_002546"
     CDS             264..491
                     /gene="feoA"
                     /locus_tag="JN691_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAY7243962.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            508..2829
                     /gene="feoB"
                     /locus_tag="JN691_002547"
     CDS             508..2829
                     /gene="feoB"
                     /locus_tag="JN691_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAY7243963.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            2829..3065
                     /gene="feoC"
                     /locus_tag="JN691_002548"
     CDS             2829..3065
                     /gene="feoC"
                     /locus_tag="JN691_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAY7243964.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            3268..4146
                     /gene="rpnA"
                     /locus_tag="JN691_002549"
     CDS             3268..4146
                     /gene="rpnA"
                     /locus_tag="JN691_002549"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HAY7243965.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPEYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMLHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            4219..4488
                     /locus_tag="JN691_002550"
     CDS             4219..4488
                     /locus_tag="JN691_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAY7243966.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            4482..4847
                     /locus_tag="JN691_002551"
     CDS             4482..4847
                     /locus_tag="JN691_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY7243967.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VTMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(4848..5636)
                     /gene="bioH"
                     /locus_tag="JN691_002552"
     CDS             complement(4848..5636)
                     /gene="bioH"
                     /locus_tag="JN691_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAY7243968.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            5674..6357
                     /gene="gntX"
                     /locus_tag="JN691_002553"
     CDS             5674..6357
                     /gene="gntX"
                     /locus_tag="JN691_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAY7243969.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRTTRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            6416..6991
                     /gene="nfuA"
                     /locus_tag="JN691_002554"
     CDS             6416..6991
                     /gene="nfuA"
                     /locus_tag="JN691_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAY7243970.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            7352..8668
                     /gene="gntT"
                     /locus_tag="JN691_002555"
     CDS             7352..8668
                     /gene="gntT"
                     /locus_tag="JN691_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY7243971.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(8713..10797)
                     /gene="malQ"
                     /locus_tag="JN691_002556"
     CDS             complement(8713..10797)
                     /gene="malQ"
                     /locus_tag="JN691_002556"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAY7243972.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10807..13200)
                     /gene="malP"
                     /locus_tag="JN691_002557"
     CDS             complement(10807..13200)
                     /gene="malP"
                     /locus_tag="JN691_002557"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAY7243973.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVLKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            13812..16517
                     /gene="malT"
                     /locus_tag="JN691_002558"
     CDS             13812..16517
                     /gene="malT"
                     /locus_tag="JN691_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HAY7243974.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELVAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(16560..17576)
                     /gene="rtcA"
                     /locus_tag="JN691_002559"
     CDS             complement(16560..17576)
                     /gene="rtcA"
                     /locus_tag="JN691_002559"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HAY7243975.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(17580..>18312)
                     /locus_tag="JN691_002560"
     CDS             complement(17580..>18312)
                     /locus_tag="JN691_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAY7243976.1"
                     /translation="GNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQD
                     QLETLPSRDLAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVR
                     QPQTLAMEEINCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRG
                     LGNEESFCSCSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDI
                     DAVMAAQSDLVEVIYTLRQVVCVKG"
BASE COUNT         4263 a         5010 c         4612 g         4427 t
ORIGIN      
        1 taaacattag ccagtccggg taattcacta ttcgaattat attttcgctg cgatataacc
       61 ttgagccaca tcaacattga gtcagattat tattcaaacc aacattcgca cacattttaa
      121 gtattgctga tagaaaccat tctcattatc attgtgttgt tgattattta atctctcctt
      181 tgttggcaaa tcatctggtc tcatgtcgcg gtcaaacgcc ccatgaggta gttatccagt
      241 taatgagaaa caagtaggca cctatgcaat acactccaga tactgcgtgg aaaatcactg
      301 gcttttcccg tgaaatcagc ccggcatatc gccaaaaact gctttctcta ggcatgttac
      361 ctggctcctc ttttgatgtg gtgcgcgtcg ctccactcgg cgaccccatt catatcgaaa
      421 cccgtcgtgt gagcctggta ttacgcaaaa aagatctggc cttattagaa gtggaagcgg
      481 tttcctgtta atacggtgat aacaacaatg aaaaaattaa ccattggctt aattggtaat
      541 ccaaattctg gcaagacaac cttatttaac cagctcactg gcgcacgtca gcgtgtaggt
      601 aactgggctg gcgttaccgt cgaacgtaaa gaagggcaat tctccaccac cgatcatcag
      661 gtcacgctgg tggacctgcc cggcacctat tctctgacca ccatctcatc gcagacctcg
      721 ctcgatgagc aaatcgcctg tcactacatt ttgagtggcg acgccgacct gctgattaac
      781 gtggtggatg cgtctaacct tgagcgtaac ctgtacctga cgctgcaact gctggaactc
      841 ggcattccct gcatcgtggc gctgaacatg ctcgacattg ccgagaagca aaatattcgt
      901 attgaaattg atgctctatc ggcacgtctt ggctgcccgg tgatcccgct ggtttcaacc
      961 cgtggtcgcg gtattgaagc gctcaagctg gcgattgatc gctataaagc taacgagaat
     1021 gtggaactgg tgcattacgc acagccgcta ctcaacgaag cagattcact ggcaaaagtg
     1081 atgccttccg acatcccgct gaaacaacgt cgctggctgg gcctgcaaat gctggaaggc
     1141 gatatctaca gccgcgccta cgccggtgaa gcgtcgcagc atctggatgc cgccctcgcc
     1201 cgtctgcgta atgaaatgga tgatccggcg ctgcacattg ccgatgcgcg ttaccagtgc
     1261 attgctgcca tctgtgatgt ggtaagcaac accctgacgg cagaacccag ccgtttcacc
     1321 actgcggtag ataaaatcgt gctcaaccgt ttcctcggtc tgccgatttt cctctttgtg
     1381 atgtacctga tgttcctgct ggctatcaac atcggcgggg cgttacagcc gctgtttgac
     1441 gtcggctccg tggcgctatt tgtgcatggt attcaatgga ttggctacac gctccacttc
     1501 ccggactggc tgactatctt cctcgcccag ggcctgggtg gcggtattaa caccgtgctg
     1561 ccactggtgc cgcagatcgg catgatgtac ctgttcctct ccttccttga ggactccggc
     1621 tatatggcgc gtgcagcgtt tgtgatggac cgcctgatgc aggcgctggg cttgccgggg
     1681 aaatcctttg tgccgctgat cgtcggtttc ggttgtaacg taccgtcggt aatgggcgca
     1741 cgtacgcttg atgcaccgcg tgaacgtctg atgaccatca tgatggcacc gtttatgtcc
     1801 tgcggcgcgc gtctggctat cttcgcagta ttcgcggctg ccttcttcgg gcagaacggt
     1861 gccctggcgg ttttctcgct gtatatgctc ggtatcgtga tggcggtgct gactggcctg
     1921 atgctcaagt acaccatcat gcgcggtgaa gcgacgccgt ttgtcatgga gctgccggtc
     1981 taccatgtac cacacgttaa aagcctgatt atccagacct ggcagcgtct gaagggcttc
     2041 gttctgcgtg ctggtaaagt gattatcatc gtcagcattt tcctgagcgc tttcaacagc
     2101 ttctcgttga gcgggaaaat cgtcgataac atcaacgact cggcgctggc ttccgtcagc
     2161 cgggtgataa cccctgtctt caagccgatt ggcgtacatg aagataactg gcaggcaacg
     2221 gttggcctgt ttacgggcgc aatggcaaaa gaagtggtgg tgggtacgct caacaccctc
     2281 tacaccgcag aaaatattca ggacgaagag ttcaatccgg cggaattcaa cctcggtgaa
     2341 gagctgttca gtgcggtaga tgaaacctgg cagagcctga aagacacctt cagccttagc
     2401 gtactgatga accccattga agctagcaaa ggcgacggtg aaatgggtac cggggcgatg
     2461 ggcgtgatgg atcagaaatt cggtagcgca gctgccgcat acagctacct gattttcgtc
     2521 ctgctgtatg taccatgtat ctcggtgatg ggagccatcg cccgtgaatc aagccgtggc
     2581 tggatgggct tctccatcct gtgggggctg aatatcgctt actcactggc aacattgttc
     2641 tatcaggtcg ccagctacag tcagcaccca acttacagcc tggtgtgcat tctggcggta
     2701 atcctgttta acatcgtagt tatcggtctg ctgcgccgcg cgcgtagccg ggtggatatc
     2761 gaactgctgg caacccgcaa gtcggtaagc agttgctgcg cagccagcac caccggtgat
     2821 tgccattaat ggcttcactt attcaggtac gtgatttgct ggcgttacgg ggccgtatgg
     2881 aagcgaccca gataagccag acattgaaca ctccgcagcc aatgattaac gccatgctgc
     2941 aacagctgga aagcatgggc aaagccgtgc ggattcagga agaacctgac ggctgcctct
     3001 ctggcagttg taaaagctgc ccggaaggca aagcctgtct gcgcgagtgg tgggcgctgc
     3061 gttaaccata ctccatcgcc tgttttgaaa agcccggtat gcgtttgcat ccgggctttt
     3121 ttgcgtgcgg ctttccataa aaatgcaatt cttgcagcgc aacgtaagtt cctctgaaag
     3181 cgtctcgcag ggatgaaaac tcgctaatgc acagatgtgg agtggcgcgt agagtcgcgg
     3241 cattcaaaca acaggtgaag gaacgccatg agtaaaaagc agagttccac cccacatgat
     3301 gcgctgttca aactcttttt acgccaaccg gagacggcac gcgattttct tgcgtttcat
     3361 ttaccggcac ccattcacgc gctctgtgat atgaaaaccc tcaagctgga gtcgagcagc
     3421 tttattgatg acgatctgcg tgaaagctat tccgatgtgc tgtggtcggt gaaaacggaa
     3481 caaggaccag aatacatcta ttgtctgatt gaacatcaaa gcacctcaaa caaactgatc
     3541 gcatttcgca tgatgcgtta cgctattgcc gcaatgcaaa atcaccttga tgctggatac
     3601 aaaacgttgc cgatggtggt gccattgttg ttttaccacg gtattgaaag cccctatccc
     3661 tattcgctgt gttggctgga ttgtttcgcc gatcccaaac tggcaaggca gctttatgcc
     3721 tccgcatttc cgctgattga tgtcaccgtc atgcctgatg atgaaatcat gctgcaccga
     3781 cgtatggcgc tgctggagtt aattcaaaaa catattcgtc aacgcgatct gatggggctg
     3841 gtagagcaaa tggcctgctt attaagtagt ggatacgcta atgacagaca aatcaaaggg
     3901 ctgtttaatt acatactgca aactggcgac gctgtacgtt ttaacgattt tatcgacggc
     3961 gttgccgaac gttcaccgaa acacaaggag agtttaatga ctattgcgga aagattgcgg
     4021 caggaggggg aacaatccaa agccctgcat atagccaaaa taatgcttga atccggagtc
     4081 cctcttgcag acatcatgcg atttaccggg ctgtcagaag aagagttggc tgcggcgagt
     4141 cagtagatac tctaccctgc ttaagtgcta ctttgtgcta tcttgcatgt aacacaaagt
     4201 agcacttagg gaactgatat gcagacagtg aaattacggc aacaaggagg agccatgatc
     4261 gttactatcc cacgagatct tgccatcgat cttggctggt caccgggcac agagcttacc
     4321 gtggagaaaa aaggcgatag cgttaatttg cgcgccacag agcacaaacc gcgtgggcgt
     4381 cttaccgtcg cgcagttgtt gagccaaatc gatgaaagcg aaattacgga gctaaatcag
     4441 tccacagaag gatgggctga agggaaaaaa ggtaatgaag catggtaaag gcacggacgc
     4501 cacatcgtgg tgagatctgg tattttaacc ctgatccggt tgccgggcat gaacttcagg
     4561 ggccacatta ttgcattgtg gtaacggaca aaaaactcaa caatgtttta aaagttacta
     4621 tgtgctgccc gatttcaaca ggggcaaatg cagcacgttc cacaggggtg acggtgaacg
     4681 tcctcccccg tgatacgcaa accggtaacc tgcatggcgt tgtactttgt caccagctaa
     4741 aagccgtcga tcttattgcc cgtggcgcta aatttcatac cgttgccgat gaaaaattga
     4801 ttagtgaagt tatcagtaaa ctggtgaatt taatcgaccc acaataatta gctctcactt
     4861 accaccaaca ccctctgctt caacgccacc agcaggtgac aaaactcgac cggatgcgaa
     4921 ataaacggcg catgggccgc tttggcgaag atatatgatt cgctgtgagg ccaaagttta
     4981 tccagcatcg gcaccacttt gcgcggcacc agaccgtcga gatagccata caatcgcaaa
     5041 aacggcatgg acacgttttg cagcggctga cggagatcga ccgttttcag gatttccagc
     5101 ccgccattaa gcacgtcaac ctccggcatc ggtaacgcca gaacggtttt cttcaacgcc
     5161 cgcgcatcct ggcgcgccgt ttcagtcccc atggtttgta acgccaggaa ccgctccact
     5221 gtacgctgaa aatcatcact gagttgctgc tgaaatcccg ccagcacgtc cggttttatc
     5281 cccggccact cgtcacgagc actaaaacaa ggtgacgacg ccacggtgac cagcgcctga
     5341 acacgctcgg gatgggttaa cgcaatctgg cttgccacca gcccgcccag actccagcct
     5401 aaccaaatgg ctttatcagg tgcctgttgc agcacggctt cggccatatc agcaagtgac
     5461 agcgcaccaa atccccggct acgcccgaag ccgggcaggt caacaaggtg cagcgtaaaa
     5521 tgcgagctaa gttcctcgtc aatgcaacgc cacacttcgg cattcagtcc ccatccgtgc
     5581 agcagcacaa gatgaacatt cccctgacct ttggtctgcc accagatgtt attcatccgc
     5641 tattgttctc ttttgactta caaggatgaa catatgctaa cagtaccggg attatgctgg
     5701 ctatgccgaa tgccactggc gttaggtcat tgggggattt gttcggtctg ttcacgcacc
     5761 acccgcacag ataaaacgtt atgcccacaa tgtggattac ccgccacaca ctcccatctt
     5821 ccctgcggtc gctgcctgca aaaaccgccg ccctggcaaa gactggtcac ggttgccgac
     5881 tatgcgccgc cgttaagtcc gcttatccac cagcttaaat tttcccggcg cagtgaaatc
     5941 gccagcgccc tgtcacgtct gttactattg gaagtcttac acgctcgtcg caccaccggg
     6001 ttgcaattgc cggatcgcat catcagcgtt ccgttatggc agcggcgtca ctggcgtcgg
     6061 ggatttaatc agagcgattt gctgtgtcag ccgttatcac gctggttgca ctgccaatgg
     6121 gatagcgaag ccgtcacacg tacacgggcc actgcgaccc agcattttct cagtgcccgg
     6181 ctgcgcaagc gcaacctgaa aaatgccttt cgtcttgaat tgcccgtgca aggtcgccat
     6241 atggtgattg tggatgatgt cgttaccacc ggaagtaccg tcgcagagat tgcgcagttg
     6301 cttttacgca atggtgcggc gactgtccag gtctggtgcc tttgtcgaac cttgtagagc
     6361 ctcgatgatg ggcgtattat aaccaactaa aatagtcaac tattaggcca ttactatgat
     6421 ccgtatttcc gatgctgcac aagcgcactt tgccaaactg ctggcaaatc aggaagaagg
     6481 gacacaaatc cgcgtatttg tgattaaccc tggcacgcct aacgctgaat gtggtgtttc
     6541 ttattgtccg ccggatgccg tggaagccac cgacactgcc ctgaaatttg acctgctgac
     6601 cgcgtatgtt gatgagttaa gcgcaccata cctggaagat gcagagatcg actttgttac
     6661 cgaccagttg ggttctcagt taacgctgaa agccccgaac gccaaaatgc gtaaagtggc
     6721 agacgatgca ccgctgatgg agcgcgtgga gtatatgctg cagtcgcaga tcaacccaca
     6781 gcttgctggt cacggtggtc gcgtttcgct gatggaaatc accgaagacg gttacgccat
     6841 tctgcaattt ggcggcggct gtaacggttg ttccatggtc gatgtgacgc tgaaagaagg
     6901 gatcgagaag cagctgctga acgaattccc ggagctgaaa ggtgtgcgcg atctcaccga
     6961 acaccagcgc ggcgaacact cctactacta agttatcccc tcttttgtag attgcccgat
     7021 gcgacgctaa agcgtcattt cgggcctacg ttgaacatgt gccagacgtt ggcacagcat
     7081 tgctttggca aatccctccc ttcgtttccc cgcgataata tgaccaacct ctcataattt
     7141 aaatttaccc cgctctggtg attctcaaac gccagatgtt acccgtatca ttcacatggg
     7201 taccaaacat actcctgaca tctgactaca ataattagtt ttagtgggta tcagtcgtgg
     7261 tgccgcaata tctctgttcc cgattgggat aattagagtt tgtcgtcaga aaattgacgt
     7321 tacccataac aaatgaaagg ccaggtaaat catgccatta gtcattgttg ctatcggtgt
     7381 aatcttgttg ttgctcctga tgatccgctt caaaatgaac ggcttcatcg ctctcgtcct
     7441 cgtggcgctt gctgttggat taatgcaagg aatgccgctg gataaagtta ttggctccat
     7501 caaagccggt gtcggcggga cgctcggtag ccttgccctg atcatgggtt ttggcgcaat
     7561 gctgggcaaa atgctggcag actgcggtgg cgcacaacgt atcgccacca cgctgattgc
     7621 caaatttggt aaaaagcaca tccagtgggc ggtggtgtta accggtttta ccgttggttt
     7681 tgccctgttc tatgaagtgg gcttcgtgct gatgctgccg ctggtgttta ccatcgcggc
     7741 ttccgcgaat attccattgc tgtatgttgg tgtaccaatg gcggctgcac tgtctgtgac
     7801 ccacggcttc ctgccaccgc atccgggccc gactgcgatt gccaccattt tcaatgccga
     7861 tatgggtaaa accctgctgt acggtactat tctggcaatc ccgaccgtga ttctcgccgg
     7921 tccggtttac gctcgcgtgt tgaaaggtat cgataagcca atcccggaag gtctctacag
     7981 cgcgaaaacc ttcagcgaag aagagatgcc gagctttggc gtcagcgtct ggacctctct
     8041 ggtgccggtt gtgctgatgg cgatgcgtgc gattgccgaa atgatcctgc cgaaaggtca
     8101 cgctttcctg ccggtagcgg agttcctcgg tgacccggta atggcaacgc tgattgccgt
     8161 gctgattgcg atgttcacct ttggtctgaa ccgtggtcgt tcaatggatc agattaacga
     8221 cacgctggtt tcttccatca aaatcattgc gatgatgctg ttgatcatcg gtggtggcgg
     8281 tgcgttcaag caggtgctgg tagacagcgg cgtggacaaa tacattgctt ccatgatgca
     8341 cgaaaccaac atttctccgc tgctgatggc ctggtcgatt gctgccgtac tgcgtatcgc
     8401 gctgggttcc gcaaccgttg cggcaatcac tgcgggtggt atcgcggcac cgctgattgc
     8461 aacgacgggt gttagcccag agctgatggt tattgcggtg ggttccggta gtgtgatttt
     8521 ctctcatgtg aacgatccgg gcttctggct gttcaaagag tactttaacc tgactatcgg
     8581 cgagaccatc aaatcctggt cgatgctgga aaccattatc tcggtgtgcg gtctggtagg
     8641 ctgtctgctg ctgaatatgg tgatttgata acactgactg ccggatgcat cgcatccggc
     8701 atcaaccgca ctctacttct tcttcgctgc agctctgcgc cgtctgtcca aatccttcag
     8761 caacttgttc acgccatcat cggcaaacat cgactcaagc gttgcggaaa gcttgcgtcg
     8821 ccagttttta tactggtaac tggtgccagg aatattcacc ggttcggcca tatccagcca
     8881 gtcttccggc tgtagtccta acagagcact gttactgtcg gcaatgtagc gctgcaaacc
     8941 acggttcagc gtcggcgtca tcgacatcaa cgatgcctta tgcccggcac gttttggcag
     9001 acaaccatat ttatgcagtg catccagcag cccttgcttc gccagttcgc gatcctgata
     9061 cagaccgcgc agtaccactt catccggata cagccccagg gttttgccca gcgttagatc
     9121 cccgctctcc cagtaaccac gcagcgttgg caggtcatgt gtcgccgcaa ccgccatcga
     9181 ctgctccgga tacgctttcg gtgcacggaa cgtcttctcg tggtcgtttt cgaaatagag
     9241 cactttgtaa gagtacacac cgctgctgcg cagcttaccg acaatctcta ccggtacggt
     9301 accgagatct tcaccaatca ccatacagcg atgacgttta ctttcgagtg ccagaatcga
     9361 gagcagatca tccaccggat agtgaacata cgcgccctga tctgccgtct cgccatacgg
     9421 tatccaccac aaacgcagca tcgacatcac atggtcaatt cgtaatgcgc cgcagttttg
     9481 catattggca cgcaacagct cgataaacgg ttcataggca cgcgcggtga tgatatgcgg
     9541 gtccattggc ggtaatcccc agttctgccc caacgggccg aggatatccg gcggcgcgcc
     9601 aaccgatgct ttcaggcaat atagttcacg gtcacaccag gtttccgccc caccttccgc
     9661 tacgccaacc gccagatcac gatacaagcc aatcggcatt tcatagccct ggcttatctc
     9721 ccagcaggcg gcaaactggc tgtaagccag ccactgcaac cagagataaa aatcgacgtc
     9781 atcacgatgt tcttcgcaga actgacgcac ttctggtgaa tccacgttct tatacatctc
     9841 tggccatgca ggccagcccc agcgcatttc gtcctctttc acttgctggg catgtagcgc
     9901 atcaaaggct gcctgccaga acaggctgtc gccctgctct gcaacaaact ggcgaaacgc
     9961 ggccatctgc tcatcatcac gttgcgcgaa acctttccac gccattcgta atgctgtcat
    10021 ttttagggcg gtaaccgtgg agtaatcgac ccagtcggca tcgcgcgcct gttgcagcgt
    10081 ctgttgcgtg gtcggcaact gccaccaggc ctgagcctct tcgctaagat ggaaatcttc
    10141 aacggcgtta acgtcgatat aaatcacatt cagccaacgg cgagaagacg ggctgtatgg
    10201 gctggcgctc tccggatttg ccggatagag cgcatgaatc gggttcaggc caatgaacga
    10261 cccgccacgt tttgccacat ccaccagcat cgctttgaga tcgccaaaat ccccaatacc
    10321 ccagtttttt tccgatcgca gcgtataaag ctgaacgcag gccccccaca gcttttgttt
    10381 attcagcaaa gcctgcggtt cgtaacaacg tttcggggcg acaatcaccc ggcaatgcgc
    10441 gcgctggtcg tcctgggtga gtgttagcgt gtgataacct tccggcagct tcgtcggtag
    10501 attgaacgct ttgccccccg ttacatggcc tttgtactgc gttccttctt cggtggtcag
    10561 ctgccagcta tattcgccgc tgccctccac caccatcggc atttttttgc cgctggtata
    10621 aaccatgaca ttcgggactg gcgttaccgc cactttcgtg gcggtacgtt gatgcatcgc
    10681 gtcaagcaaa cgccgtttgg tttcggcgct aatcgactgc ggtttaccgt gggcattgat
    10741 gtaattgggg ctaatccccg ccgccagcgc ggcattatcc agacgtttgc tttccatcga
    10801 gcttccttag cgttttgcct gccagatacg agcctgataa tcgcgaatag agcgatccga
    10861 gctaaacata ccgcagcggg cggtattgag gatcgccgcg cgagtccagg cctcctggtc
    10921 gcggtacagc acatccacct gcttttgtgc ctcaacatag gctgcgaaat ccgccatcac
    10981 cagatacgga tcgccgccct gtttgccgat actgtgcagc atctggtcga aggcatgctt
    11041 atcgccgtcg ctgtatttac cgctttccag ctctttcaat actgcgtcca gcaccttatc
    11101 tttcttccgc catttcaccg ggtcgtagcc tttggccaga atagccttca cttgttccac
    11161 ggtatgacca aaaataaaga tattttcttc accgactttc tcggcgattt caacgttcgc
    11221 accatccagc gtaccgacag taagcgcacc attgagcgcc agtttcatat tgccggtacc
    11281 ggaagcttct ttacctgcag tcgaaatttg ttcggagata tccgccgccg ggatcagttt
    11341 ttccgccgcc gaaacgcaat aatccggcag gaacaccacc ttcaacttat cgccaaccag
    11401 tggatcgttg ttgatcacgt cagccacttt gttgatcgca aagataatat tcttcgccag
    11461 gtagtagccc ggtgccgctt tcgcgccgaa gaggaagacg cgcggtacgc gatcagcctg
    11521 cgggttttca cgaatttctt tgtacaacgc cagaatatgc agcagattca ggtgctggcg
    11581 tttgtactca tgcagacgtt tgatctgaat atcgaaaatc gcctgtggat tgatctcaat
    11641 accggtacga actttcacaa actccgccag acggacttta ttcgcctgct tgatctcgcg
    11701 atattgctga cggaatttcg catcatcagc gaatttttcc agattgatca gctgatcgag
    11761 atcgttagcc cactcttttt gcagtgattt atccaacaga gccgccagtg cagggttgca
    11821 ctgtttgatc cagcgacgtg gggtaatacc gttggtgacg ttatggaatt tgttcggcca
    11881 tagctggtga tattccggga acagatcttt caacaccaga tccgagtgca gcgccgcaac
    11941 accgttcacc gcgaaaccgc caaccacaca caggttcgcc atatgcactt gtttatcgtg
    12001 caccaccgcc agtttggccc acactttttc atcgcccggc caggttttct ccaccagcgt
    12061 tttaaagcga gtattaattt cgttaataat ctgcatatgg cgcggtagta agcctttcac
    12121 cagtttcaca tcccagcgtt ccagcgcttc tggcatcagg gtatggttgg tgtaagcgaa
    12181 agttttgctg gtgatggccc aggcgtcatc ccagctcatc tggtgctcat cgatcagcac
    12241 gcgcagcagt tctggaatcg cgatagttgg atgggtatcg ttcagctgaa taacttcgta
    12301 atccgccagt tcgtgcagtt tacgccccgc cagatgatgg cgacgcaaaa tatccgctac
    12361 cgaacaggca cactggaagt attgctgcat caggcgcagc tttttaccgg cagtatggtt
    12421 gtcgtttgga tagagaactt tggtcagttt ttccgcattg atgccttgct gttcggcgcg
    12481 caggaaatca ccgtcgttaa atttagttag atcaaacgga tgcgcatgcg tcgcctgcca
    12541 cagacgcagc ggctgcgcca cgccattacg atagccgaca acggggagat cccacgcttg
    12601 accggtaatg gtaaactccg gctcccagcg tccgtctttc atcactttac cgccaatccc
    12661 tacctgcaca tccagtgctt cgttgtggcg gaaccacggg tagttactgc gatgccagtc
    12721 atccggcgct tcaacctgtt tgccatcgac aaaagactgg cggaacaaac catattgata
    12781 gttcagaccg taacccgtcg cagactgacc gacagttgcc attgagtcga ggaagcacgc
    12841 cgccagacgt cccagaccac cgttacccag cgccgggtcg atctcttctt ccagcaggtc
    12901 agtcaggttg atgtcataag ccttcaacga atcctgtaca tcctgatacc agccgagatt
    12961 caacaggttg ttgcccgtca gacgaccaat caaaaactcc attgagatgt agttaacatg
    13021 tcgctgattc gccaccggtt tggcgaatgg ctgagcacgc agcatttcgg ccagtgcttc
    13081 actcactgcc agccaccact ggcgaggagt catttcagcc gcagaattta agccataacg
    13141 ctgccactga cgtgaaagcg cttcctgaaa ttgcttatcg ttaaaaatag gttgtgacat
    13201 aggagttcca cttttcttag attttcaaca caacgttatc gctagtttgc caggctcgat
    13261 gttgaccttc ctcatcctgc gggggattag gcagggagga gttgcgggga tgagcaagga
    13321 aatgtgatct cgaccactta aagctagtgc aaaccacagg attagcagca aatcaatgcc
    13381 atacagcgca gaaaatctgt atctaagtgc aaaaaatggc cgttgcgtat tttcaaaaag
    13441 cggaaggtaa ctctataaat taagtaaagg agtgaaacag tttcacaagt aaaatatcca
    13501 gtgtgctcca tctcattctt aatagattta ttaagatcat ctttttagat ggcactttca
    13561 tcaggaatga agaagaaacc cttgcttaaa tgaatctgat gaacataagg gaaaccagta
    13621 ttcacgctgg atcagcgtcg ttttaggtga gttgttaata aagatttgga attgtgacac
    13681 agtgcaaatt cagacacata aaaaaacgtc atcgcttgca ttagaaaggt ttctggccga
    13741 ccttataacc attaattacg aagcgcaaaa aaaataatat ttcctcattt tccacagtga
    13801 agtgattaac tatgctgatt ccgtcaaaac taagtcgtcc ggttcgactc gaccataccg
    13861 tggttcgtga gcgcctgctg gctaaacttt ccggcgcgaa caacttccgg ctggcgctga
    13921 tcacgagtcc tgcgggctac ggaaagacca ccctcatttc ccagtgggcg gcaggcaaaa
    13981 acgatatcgg ctggtactcg ctggatgaag gtgataacca gcaagagcgt ttcgccagct
    14041 atctcattgc cgccgtgcag caggcaacca acggtcactg tgcgatatgt gagacgatgg
    14101 cgcaaaaacg gcaatatgcc agcctgacgt cactcttcgc ccagcttttc attgagctgg
    14161 cggaatggca tagcccactt tatctggtca tcgatgacta tcatctgatc actaatccag
    14221 tgatccacga gtcaatgcgc ttctttattc gccatcaacc agaaaatctc accctggtgg
    14281 tgttgtcacg caaccttccg caactgggca ttgccaatct gcgtgttcgt gaccaactgc
    14341 tggaaattgg cagtcagcaa ctggcattta cccatcagga agcgaagcag ttttttgatt
    14401 gccgtctgtc atcgccgatt gaagccgcag aaagcagtcg gatttgcgat gacgtttccg
    14461 gttgggcgac ggcactacag ctaatcgccc tctccgcccg gcagaatacc cactcagccc
    14521 ataagtcggc acgccgcctg gcgggaatca atgccagcca tctttcggat tatctggtcg
    14581 atgaggtttt ggataacgtc gatctcgcaa cgcgccattt tctgttgaaa agcgccattt
    14641 tgcgctcaat gaacgatgcc ctcatcaccc gtgtgaccgg cgaagaaaac gggcaaatgc
    14701 gtctcgaaga gattgagcgt caggggctgt ttttacagcg gatggatgat accggcgagt
    14761 ggttctgcta tcacccgctg tttggcaact tcctgcgcca gcgctgccag tgggaactgg
    14821 tggcggagct gccggaaatc caccgtgccg ccgcagaaag ctggatggcc cagggatttc
    14881 ccagcgaagc gattcatcat gcgctggcgg caggcgatgc gctgatgctg cgcgatattc
    14941 tgcttaatca cgcctggagt ttgttcaacc atagcgaact gtcgctgctg gaagagtcgc
    15001 ttaaggccct gccgtgggac agtttgctgg aaaatccgca gttagtgtta ttgcaggcgt
    15061 ggctaatgca aagccaacat cgctatggcg aagttaacac cctgctagcc cgtgctgaac
    15121 atgaaatcaa ggacatcaga gaaggcacca tgcacgcaga atttaacgct ctgcgcgccc
    15181 aggtggcgat taacgatggt aatccggatg aagcggaacg gctggcaaaa ctggcactgg
    15241 aagagctgcc gccgggctgg ttctatagcc gcattgtggc aacctcggtg ctgggtgaag
    15301 tgctgcactg caaaggcgaa ttgacccgct cactggcgct aatgcagcaa accgaacaga
    15361 tggcacgcca gcacgacgtc tggcactacg ccttatggag tttaattcag caaagtgaaa
    15421 ttctgtttgc ccaagggttc ctgcaaaccg cgtgggaaac gcaggaaaaa gcgttccagc
    15481 tgatcaacga gcagcatctg gaacagctgc caatgcatga gtttctggtg cgcatccgag
    15541 cacaactgtt atgggcctgg gcacggctgg atgaagccga agcgtcggcg cgtagcggga
    15601 ttgaagtctt gtcgtcttat cagccacagc aacagcttca gtgcctggca atgttgattc
    15661 aatgctcgct ggcccgtggt gatttagata acgcccgtag ccagttgaac cgtctggaaa
    15721 acctgctggg gaatggcaaa tatcacagcg actggatctc taacgccaac aaagtccggg
    15781 tgatttactg gcaaatgacc ggcgataaag ccgccgctgc caactggttg cgtcatacgg
    15841 ctaaaccgga gtttgcgaac aaccacttcc tgcaaggtca atggcgcaac attgcccgcg
    15901 cacaaatctt gctgggcgag tttgaacctg cggaaattgt tctcgaagaa ctcaatgaaa
    15961 atgcccgcag tctgcggttg atgagcgatc tcaaccgtaa cctgttgctg cttaatcaac
    16021 tgtactggca ggccggacgt aaaagtgacg cccagcgcgt gttgctggac gcattaaaac
    16081 tggcgaatcg caccggattt atcagtcatt ttgtcatcga aggcgaagcg atggcgcaac
    16141 aactgcgcca gctgattcaa cttaatacgc tgccggaact ggaacagcat cgcgcgcagc
    16201 gtattctgcg agaaatcaat caacatcatc ggcataaatt cgcccatttc gatgagaatt
    16261 tcgttgaacg tctgctaaat catccggaag tgccagaact aatccgcacc agcccgttga
    16321 cccaacgtga atggcaggtc ctggggctga tctactccgg ttacagcaat gaacaaattg
    16381 cgggcgaact ggaagtggcg gcaaccacca tcaagacgca tatccgcaat ctgtaccaaa
    16441 aactcggcgt cgcccaccgt caggctgccg tgcagcacgc gcagaaactg ctgaagatga
    16501 tgggttatgg ggtgtgagtt tagccggata atgcattgga tccggtttac atctttacat
    16561 cattcaatac tcacccgcgt tacgccatct gtttctatca aaccaaaccg caccggcaag
    16621 aaacgctcca ccaccgcgat attggtcagc agatggcatg aaggatgggc gaccgtaaat
    16681 tctcccgcgc ccgccagcgc catcggcagt accagttggt cggcgagata ttcccccacc
    16741 gccgctgggc ttgccaggta gcgtttcacc tctttcacta actgcgcggc taccacctcg
    16801 gcactgacgc gcttttcacc gacgacgaaa aagcgttcag tgatattttc actttcgact
    16861 tccagcgaga cggtattacc cggcccctgg tcgcgcggca ggttatgaat attctgttca
    16921 tgcagggaaa aactccccgc cagtgtagcg atttcacgct cagcaacatg gcgcggcaca
    16981 ccagctaata gaacttctcc acgcatctgc acaatgttcc cgcgctcgcc aagttgcaag
    17041 gtgttaaacg atgccacggg cgagacttcc gttgccacca caccgcctcc ggcaggataa
    17101 aaaccgtgac gtaacagcgt ggtttgctga tgaattccta ttttcgccag cagcggctcc
    17161 agcacccggc ggataaaatc cgcaggcggc gccgacgggt tatcggtgcc gccgctcact
    17221 tcaacacgcg aaggtccatc ggcaaaccac agcgcgggca gcaccgtttg cagcaccagc
    17281 gtacaacttc cggcgctacc gatagcaaag cggtaatcgc cgccgcgcac ggtgccaggc
    17341 cggaagacca gacgctgcga tcccagctcc gcgccttcca ccgttgccct acaaatttcc
    17401 gcagccgctt ttaccgcggt cagatgctgg cgcagcagcc ccggtttcgc ccgtccggct
    17461 cgaatgccag tgatggtaaa tggctggccg gttatcatcg acaggctcag cgccgagcgc
    17521 aggatctgcc cgccgccttc gccctgtgcg ccatccagcg caatcatcct tttcatcatt
    17581 tatcctttta cgcacaccac ctgacgcagg gtgtagataa cttccaccag atcgctttgt
    17641 gccgccatca ccgcatcaat atctttatac gccatcggga tttcgtcgat cacttcggca
    17701 tctttacggc attccacatg cgcggtggca cgaatttggt cttccacgct gaacagtttt
    17761 ttcgctttag ttcggctcat tacccgcccg gcaccgtggc tgcacgaaca gaacgactct
    17821 tcatttccca gcccacggac gataaagctt tttgctccca tcgaaccggg aataattcca
    17881 tattgaccag cacgcgcaga caccgcacct ttacgcgtca cgtagatctc ttcaccaaag
    17941 tgctgttctt tttgcacata gttgtggtga cagttgatct cttccatcgc cagcgtttgt
    18001 ggctgtctga ccgttttctg cgtaatgctc tgcaacgccg ttaccacatt ttccatcatc
    18061 gcatcgcggt taaggctggc gaaaagctgc gcccaggcca cggctcttag gtaatcatca
    18121 aagtattccg taccttccat aaagtacgcc agatcacgcg acggcaacgt ctcaagttgg
    18181 tcctgcatct ctttttgcgc cagatcgata aagtaagtcc cgatggcgtt accaattccg
    18241 cgtgaaccgg agtgcagcat aatccacacc tgctccgact catcaaggca gatttcaata
    18301 aagtggttac cc
//
LOCUS       DADLSA010000078        18204 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.078, whole genome shotgun sequence.
ACCESSION   DADLSA010000078 DADLSA010000000
VERSION     DADLSA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.078"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            481..1137
                     /gene="pphA"
                     /locus_tag="JN691_002561"
     CDS             481..1137
                     /gene="pphA"
                     /locus_tag="JN691_002561"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAY7243977.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIVLADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(1138..1413)
                     /locus_tag="JN691_002562"
     CDS             complement(1138..1413)
                     /locus_tag="JN691_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAY7243978.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRHGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(1434..1670)
                     /locus_tag="JN691_002563"
     CDS             complement(1434..1670)
                     /locus_tag="JN691_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAY7243979.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1788..3227)
                     /gene="rsmF"
                     /locus_tag="JN691_002564"
     CDS             complement(1788..3227)
                     /gene="rsmF"
                     /locus_tag="JN691_002564"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HAY7243980.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(3307..5940)
                     /gene="yebT"
                     /locus_tag="JN691_002565"
     CDS             complement(3307..5940)
                     /gene="yebT"
                     /locus_tag="JN691_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HAY7243981.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDALIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5909..7192)
                     /gene="yebS"
                     /locus_tag="JN691_002566"
     CDS             complement(5909..7192)
                     /gene="yebS"
                     /locus_tag="JN691_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HAY7243982.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAE
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7322..7819
                     /locus_tag="JN691_002567"
     CDS             7322..7819
                     /locus_tag="JN691_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAY7243983.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7916..8614
                     /gene="proQ"
                     /locus_tag="JN691_002568"
     CDS             7916..8614
                     /gene="proQ"
                     /locus_tag="JN691_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAY7243984.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8634..10682
                     /gene="prc"
                     /locus_tag="JN691_002569"
     CDS             8634..10682
                     /gene="prc"
                     /locus_tag="JN691_002569"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAY7243985.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQTAPVK
                     "
     gene            10874..11755
                     /gene="htpX"
                     /locus_tag="JN691_002570"
     CDS             10874..11755
                     /gene="htpX"
                     /locus_tag="JN691_002570"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAY7243986.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(11801..13174)
                     /locus_tag="JN691_002571"
     CDS             complement(11801..13174)
                     /locus_tag="JN691_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7243987.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            13351..14142
                     /gene="kdgR"
                     /locus_tag="JN691_002572"
     CDS             13351..14142
                     /gene="kdgR"
                     /locus_tag="JN691_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAY7243988.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(14285..14524)
                     /locus_tag="JN691_002573"
     CDS             complement(14285..14524)
                     /locus_tag="JN691_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243989.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            14683..14826
                     /gene="mgrB"
                     /locus_tag="JN691_002574"
     CDS             14683..14826
                     /gene="mgrB"
                     /locus_tag="JN691_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAY7243990.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            14901..15188
                     /locus_tag="JN691_002575"
     CDS             14901..15188
                     /locus_tag="JN691_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7243991.1"
                     /translation="MTEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            15858..16001
                     /locus_tag="JN691_002576"
     CDS             15858..16001
                     /locus_tag="JN691_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAY7243992.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            16014..16223
                     /gene="cspE"
                     /locus_tag="JN691_002577"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             16014..16223
                     /gene="cspE"
                     /locus_tag="JN691_002577"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY7243993.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            16389..17198
                     /gene="rlmA"
                     /locus_tag="JN691_002578"
     CDS             16389..17198
                     /gene="rlmA"
                     /locus_tag="JN691_002578"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAY7243994.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPEPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(17195..17761)
                     /gene="mntP"
                     /locus_tag="JN691_002579"
     CDS             complement(17195..17761)
                     /gene="mntP"
                     /locus_tag="JN691_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAY7243995.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPHRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4821 a         4348 c         4584 g         4451 t
ORIGIN      
        1 cccaaatcgt aaaggaaacc gtagcacgtc gtatgcaaga acgtgccacg gctggctgat
       61 ggatgttcga tagcgcgagt ttgaatgaaa atcagccgga gatgatttta cataattgct
      121 acggaattat tcaatacagg aattgcttgc gtctgcatgg attgacctga aatattcccg
      181 aaaatttctc taaaaaactc gaaaaaaatg gtaactggtt gaatgtatta atatgcaatg
      241 gtacgtgtca gggattaaaa gatgaacgta aatttattca acgcattaat tttaaagggt
      301 tttattgttt gttgacgaaa acaggaatcg tgttcggtct ctttttatct gttaaaagcc
      361 agaagcattt ccttcgctga ctttatagtc aaccataaca cacactctac tgtctgagtc
      421 cagcgttttt taacattctt gttaagatta tgtgatcttt agcgcgggag gaaaatattg
      481 atgaaacagc ctgcgcccgt ttatcagaga attgcgggtc atcaatggcg acatatctgg
      541 ctttctggcg atatacacgg ttgtcttgag cagttgcgcc gcaaattatg gcattgtcgt
      601 tttgatccgt ggcgagattt acttatctca gtgggagacg ttatcgatcg tgggccgcaa
      661 agtttacgtt gtttgcagtt actggaacaa cattgggttc gtgcggtaag aggcaatcat
      721 gaacagatgg cgatggatgc gctggcattc cagcagatgt ctttgtggtt gatgaatggc
      781 ggcgactggt ttattgtgct ggcagataat caacagaaac aagcgaaaac ggcgctggaa
      841 aaatgtcagc atttgccctt tattcttgaa gtacacagtc gcaccggcaa gcatgttatt
      901 gctcatgccg attatccaga tgatgtttat gaatggcaaa aggacgttga tttgcatcag
      961 gtcttgtgga gccgctcgcg attaggtgaa cgcccaaaag ggcagggaat tacaggtgct
     1021 gatcatttct ggtttggtca tacaccgttg cgacatcgcg tggatattgg caacctgcat
     1081 tatattgata ccggtgctgt ctttgggggc gaattgactc ttgtgcaact gcaataatta
     1141 aaaatcacca tactcctgtg ctggtcgcca gaaaccatct ataaaatcct caatcggaaa
     1201 acaaccgcca tggcggatcc gttgatcgct catagaataa agacactgct gttccgtgtt
     1261 gtagacatcc acaacaatat cttcacaacc gccatccagg tagcaaacaa aaagtaccag
     1321 cgcgaacatt tcatccccga agtgtggtgc cgtaccgtta agtttaggag agattttaca
     1381 acgggggaat aaccaggaca aataacccgc catgccgacg ggttattttt ggatcaggca
     1441 agacgcataa tccaggcatc agattgttga tcgtaacggg tacggtaaag gacagagtgt
     1501 tcgccattta gcagggcagg gatactggtt tcagcatccc aggcatccag ttgcatacat
     1561 aaatcggggt cagatttaca aggaatggtt aacgttcgat cacccggcga ttcaccgtgt
     1621 aattcgccgt cgtcgatttc aaaagcgcct atgcgcacac tggttttcat cttcgtgctc
     1681 tccatttagc tgccgattgt ggtacgacca gtccgatcgc acaatttttc agcctagttg
     1741 aggccgccga atgtgccagt caaaaagtgc gctttttttg tgcgctgtca tgcgttactg
     1801 gtaaaaagtt tcccgtcccg caccagttca cgcgggtagc tgtttttcag tcgcgaacca
     1861 atccgtttgg ctaaaccaat cggctgatgc tggaaagtaa ccaacacgtc atccgccact
     1921 ggcgcggctt gcgggtaaac atcgcgcccg cgataccact cctccgcttc ctgcggtgtc
     1981 agttcaaaag cgtttacatt gtcgggggag gcatgggcaa taaccgcttc atgctgccag
     2041 cgataacctt tgttatgcgt ttcggcaagt ttaatcccca agcgagaaaa tcggacttta
     2101 ccgatcaggg cttcaatgcc taccgggaac aaccacagtt ctttgtcgcg ttgccagagt
     2161 cgcaggtttc catcccagtt taagccaaca cttgcagccg cctgacgaat ttgtccggct
     2221 tcgcgatctt tcaccgggct gaacgggaaa ttgcccactt tgtattttgg ggcgggtaag
     2281 gcggggatcg cctgagtttt acgcagacga gcaacgaaga agccttcgca gtcgtaaatt
     2341 tgtgggaaaa catgcaaaaa accttcttcg gtcagcgcct tgtttgcacc agggaagaga
     2401 tcgccaagtg gtaaaaactc tacttcgtcg gggtaagtct ctttcagcca ccggcaaacg
     2461 gcttcgtttt cttcccggtt taaggtacag gtcgagtaaa ccagcgtacc gccaggacgt
     2521 aatgcatgaa aggcgctgtc aatcagttcc cgttgggtag ctgcgatttc ctgattgctt
     2581 tcgggtgacc agttttttag cgcatcggga tctttacgca ccacgccttc gccggagcag
     2641 ggagcgtcca gcaaaatggc atcaaacatt tctggcactg ccgcaccaaa cacgcggcca
     2701 tcaaaatgtg tgagcgcaac attactgatg ccacagcggc tgatattggc atgtaacact
     2761 tttacccgac tggcggaaaa ctcattggca aggattgcac cttcgttatt catccgcgcg
     2821 gcaatttgcg tcgtttttga gcctggcgca gcagcgacat ccatcacccg ctgtggtgca
     2881 ttaccgtcag caaacaaggc ggcgacgggc aacattgaac tggcttcctg aatataaaac
     2941 aggccactta aatgctcggt ggtactaccc aatggcaatg catcttcatt gtcgcgttca
     3001 atccagaaac cttcttcaca ccacggaatt ggcgtaagcg tccagccata aggagcggtt
     3061 aattgcagga aatcagcaac ggagattttc agcgtattaa cgcgaatgct gcggcgcaac
     3121 gggcgctgac aggcggcaag aaaatcatca aatgagagcg tcgaaggcat agcttcgcgc
     3181 atttgtgtca gaaacgcgtc cgggaaataa acggtgtgtt gggccacgag catgtaccac
     3241 tggcaaaaaa acaggcgcgc agtttagcat aaacgctccg gcgcaggcac gccggagcag
     3301 tgggcattat ttgggaagcg cagttcccca ttcacgccac tctttcggtt cactttcctg
     3361 caacaggaag tgtttgcctt cctgggcttt cggtgcaagt ggcgtacccg gaggcgtggc
     3421 gaaggcgatg ccgccacgga taaattggtt aaaggtgccg gttttcacta cgccgcccgt
     3481 cagaccaaag tccagactgt aacccgatgc caaccagaag acggaattgt tacgcaccag
     3541 gtgttgatag cgtttactga tgcgcatcgc aatcatcacg cgatctgaca atgtccccag
     3601 cgtcattccg gtaaccgtac ctacttccag accacggaac agcacaggcg taccgatgcc
     3661 taacgaaccg gcttccggcg cttcaacaat aatgcttaaa ccatctaggt aacgcgaatc
     3721 agtaatggtg gcctcttgta attcaaagtc gcgacgagga ttgccacggc ctggttcgac
     3781 gttgatatac ggctggagga tagtatctag atgctcaacg ccagctgccg agatttgcgg
     3841 tgtgaccact gagaagcgcg taccaccgcg agcaaaggtc tggacatatt ccggatagag
     3901 caccgccttt gcctgtactt cattgcgtgc ggtaatcaga tccagcgtct ggatttgccc
     3961 gatatcaata ccgagatagc gaattggcat cccgaccgcc agttttccgg catcgaaagc
     4021 gtgaagcgta atctgcccgc caaccgcacg ggccgctgtt tcggaagcat acagaattcg
     4081 cttgtcgcct ttacgctgac tggcgctggc accactgagg ttatcgaagc taatggcccc
     4141 ctttaatgct ctggagagcg gggatgcctg tacggtcagg ccactaccat tcagctgaac
     4201 tttcgccccg ccttctgccc agaacacgct gttgctggtc aaaaggttgc gatattccgg
     4261 tttaatgtgc agatcgatat cgaacgcgtt agctcgcgga cgcacggtaa taacttcacc
     4321 aacttcaaat ttacggtaca gcactaccga tcctgcctgc acatccggca gcgtctctgc
     4381 actcaaactc acggttgtgg tgggtaaatc gctaaggctg ttctccagcg ctttttccag
     4441 attggcatac agtggatagc tggctttcat ctcgccttta tcgcccggca gaatacgtat
     4501 cccgccgtta atccattctg aggcgctggc accgagaaac tcaacgccat ccagccccac
     4561 cttcacgtcg acacggctgt tgacgacaaa tttgctatcg ccttttacca gttctcgatg
     4621 ctgaggctcg atggcgacgg taaaggtgac gcctttgctg gtgagtttac gatcgataac
     4681 ctggcctacc tgcacgccgt gaagaatgag cggctgaccc gcatcaatac cgtaactttc
     4741 cggtgctgtc agggtcagtg tcagaacgtc cggctcttgc agcagggctt tttcgcccgg
     4801 cacaacaacg aactctttgc gtggctcgcc atcgccgggc accaactcaa aggtttttcc
     4861 ggtcagcagg gcgctgagat tagcatcgct aagggataat ttcgggttgc gtaattcgat
     4921 gcgggtattt tcacgaagca gggtaacgac gctgggatca acggtcattt ccccagtgac
     4981 tttaccacca ggatttaaat ccagtttagt cagctgtccg acttccagcc cctgatacat
     5041 taacggcgtc gagtcggcgg ttaatcctgc tccactcggc agttccagtt ttattattac
     5101 tccacgctgg ctgtgggcca gatcttcata cagaccaaag gtatcttccg cctcggcagg
     5161 tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta accagtgccg ccagactttc
     5221 cagtttcacc ttcgcgccac tgatactgac gttggcatca acgccggaaa cgttccagaa
     5281 acggctacct tttttcacca gatcggtaaa acgccgctcg atcagggcat caatcaccac
     5341 gccttgcttg ttgggattga tggcatagtc gtagactttt cccaccggga tcttgcggaa
     5401 atagaccaat gaaccgctgt tcagcgaacc gagatcgggg gcttgcaggt ggatcatcag
     5461 atcgccattg tccagccgat atttcggttg ggtatcgagc gcgacaaagt gatcctgctc
     5521 tttaccttta cccggcatca tgccgatata gttcccaccg acgagggcgt ccaacccgga
     5581 gacacctgcc aacgatgctt ttggcgtcac cagccagaac tgagtctctt cgcgcagcgc
     5641 atctttcata tcggacttga tgctgacctt gacttcaatc ttacgaagat cgtcgctgag
     5701 gctgatatcc tgcactgttc cgacttcaac gccctgataa cgaacaggcg tacggcccgg
     5761 aacaatacca tccgccgaca taaagtcgat ggtgacggta ttaccccggt cctgataact
     5821 gtcccaaatc agccaaccgg caatcattag cgcgatgaaa ggcagcagcc agaaaggtga
     5881 gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa gcgggcgttt cctgactcat
     5941 gtgcatccca aagtaagcgg ctgtccagcc attccacagc aagaatagtc aaaattacgg
     6001 cagcgccaaa ataaaactca gccggtccca tagtaaaagc gaggatctga tcgcgattaa
     6061 tcagcgacat ggttaaagat atgacaaaca ggtcgagcat cgaccagcgt ccaatccagg
     6121 tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg ctggcattta aaatgaatgc
     6181 tcaacagtaa agtgaacatg acgatcactt tagtaaacgg taccagaata ctggcgataa
     6241 acacgattcc cgcaaccgca atgttgctac ttgccagcga cataattccg gaaagaatcg
     6301 tatcttcctg ccggccacca ttcagataaa tgatagaaat aggcaatagg ttggctggta
     6361 acaacaaaac gattgacgct aacagcgccg cccagcattt ttgcaaacta tgacgacgac
     6421 gcaggcgtag cgggatatgg caacgcgggc agagaccacg ctgatcagga tagccggtaa
     6481 aatggcaccc aagacagaca cgaagtttct cgtccctacg cgtagcgggg cgctgcggat
     6541 aatatcgctc ccacagctcc tcaacattaa gatgtgacaa cgtcaccgtc gttaaaatca
     6601 ccaacgccac aaaagagaac aagccgacac ccgcctggat atgggcataa tcctgtacct
     6661 ttatagaagc aacgccaatg ccgaccaggt agatatccag cattacccac tctttaagtc
     6721 gctcaagcat cagcagtacc ggacgtaaat tcattcccag tcggttacca aaccataaat
     6781 atgctatgga ggacaccaga atgagggggg caccaataac gcagaaaaag accattgatc
     6841 ccgttatcgc atcgccctgt ttggtcattt gccagatgcc ttgcatgacg ttggcgtcaa
     6901 tacgaatgcc taacagccag atatgcaaca gcggttcgcc ccaggcaaac ggcataagca
     6961 acagcatggt gaaggccatt gccgccaggc gcgttagcga ccagtcgcgc ccgtcgcgaa
     7021 tttttgcctg acagcgcgga caataggcac tttgatgaga atttatctcg ggcaggctaa
     7081 acagcatgtc acattgcggg caacgttggt aatcaccacg cggcagttcc tcgccgattg
     7141 ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt gttaagagcc atgacgtgcc
     7201 aaatgtaata agaattaaca ttaatcttaa cccatgatgg gaatgatctt gagcattaac
     7261 gcatttaatg cttattttaa taggctggca gttccaggta agacaaccaa gtgattaaat
     7321 aatgaacaaa acagaatttt acgcggattt aaatcgcgac tttaacgcgc tgatggcggg
     7381 agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg ttgttatacg agcgtctcac
     7441 tgacgttaac tgggcaggtt tttatttgct tgaggacgat acactggtac tcggaccatt
     7501 tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc ggggtgtgcg gcacagcggt
     7561 tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg tttgacgggc atattgcctg
     7621 tgatgcggcg agtaattctg aaattgttct gccgctggtg gtgaaaaatc agattattgg
     7681 tgttctcgac atcgatagta ccgtcttcgc tcgctttaca gacgaggacg agcaaggctt
     7741 acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg acggattaca aaaaattctt
     7801 tgcgagcgtc gcaggataat caacggataa cgtagcattt actggtagcg tcattataat
     7861 gacgcctgtt catgcctgcg cttgttggct acgtccgttg taatcaggaa atttcatgga
     7921 aaatcaacct aagttgaata gcagtaaaga agtaatcgcg tttctggccg aacgttttcc
     7981 ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa attggtattt ttcaggattt
     8041 ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg caattacgat ccgctttacg
     8101 tctctacact tcgagctggc gttatcttta cggtgttaaa cccggcgcaa cgcgtgtcga
     8161 tcttgacggc aacccatgcg gtgagctgga cgagcaacat gtagagcatg ctcgcaagca
     8221 gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct gaacagcaag cgaaaaaacg
     8281 cgaagctgcc gcagctgctg gtgagaaaga agacgcaccg cgccgcgaac gcaagccacg
     8341 tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa cctcgtgcgc aaaagccggt
     8401 agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa cagcacaccc cggtttctga
     8461 catttcagct ctgactgtcg gacaagccct gaaggtgaaa gcgggtcaaa acgcgatgga
     8521 tgccaccgta ttagaaatca ccaaagacgg cgtccgcgtc cagctgaatt cgggtatgtc
     8581 tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga ggccgggcca ggcatgaaca
     8641 tgttttttag gcttaccgcg ttagctggcc tgcttgcaat agcaggccag accttcgctg
     8701 tagaagatat cacgcgtgct gatcaaattc cggtattaaa ggaagagacg cagcatgcga
     8761 cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca ttatcgccag ttcgacctcg
     8821 atcaggcatt ttcggccaaa atctttgacc gctacctgaa tctgctcgat tacagccaca
     8881 atgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa gaaaaccgag ttaggcgatg
     8941 aactgcgttc aggcaaactc gacgttttct acgatctcta caatctggcg caaaagcgcc
     9001 gttttgaacg ttaccagtac gctttgtcgg tactggaaaa gccgatggat ttcaccggca
     9061 acgacactta taaccttgac cgcagcaaag cgccctggcc gaaaaacgag gctgagttga
     9121 acgcgcagtg ggacagtaaa gtcaaattcg acgagttaag cctgaagctg acaggaaaaa
     9181 cggataaaga aattcgtgaa accctgacac gccgctacaa atttgccatt cgtcgtctgg
     9241 cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac ggcgtttgcg cgtgaaatcg
     9301 atccgcatac caactatctt tccccgcgta ataccgaaca gttcaacact gaaatgagtt
     9361 tgtcactgga aggtattggc gcagtgctgc aaatggatga tgactacacc gttatcaatt
     9421 cgatggtggc aggtggtccg gcagcgaaga gtaaagctat cagcgttggt gacaaaattg
     9481 tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat tggctggcgt cttgatgatg
     9541 tggttgcctt aattaaaggg ccgaagggca gtaaagttcg tctggaaatt ttacctgctg
     9601 gtaaagggac caagacccgt actgtaacgt tgacccgtga acgtattcgt ctcgaagacc
     9661 gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa agtcggcgtg ctggatattc
     9721 cgggcttcta tgtgggtttg acagacgatg tcaaagttca actgcagaaa ctggaaaaac
     9781 agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg cggtggggcg ttgaccgaag
     9841 cggtatcgct ctcaggtctg tttattcctg cgggtcccat tgttcaggtc cgcgataaca
     9901 acggcaaggt tcgtgaagac agcgataccg acgggcaggt gttctataaa ggcccgctgg
     9961 tggtactggt tgaccgtttc agtgcttcgg cttcagaaat ctttgccgcg gcaatgcagg
    10021 attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg taaaggcacc gttcagcaat
    10081 accgttcatt gaaccgtatt tacgatcaga tgttacgtcc tgaatggcca gcgctgggtt
    10141 ctgtgcagta cacgatccag aaattctatc gcgttaacgg cggcagtacg caacgtaaag
    10201 gcgtaacacc agacatcatc atgccgacgg gtaatgaaga aacggaaacg ggtgagaaat
    10261 tcgaagataa cgcgctgccg tgggatagca tcgatgccgc gacttatgtg aaatcaggag
    10321 atttaactgc ctttgaaccg gagctgctga aggaacataa tgcgcgtatc gcgaaagatc
    10381 ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa cgctatgaag gacaagcgca
    10441 atatcgtttc tctgaactac gctgtgcgtg agaaagagaa taatgaagat gatgcgacgc
    10501 gtctggcgcg tttgaacgaa cgctttaaac gcgaaggtaa accggagttg aagaagctgg
    10561 atgatctacc gaaagattac caggagccgg atccttatct ggatgagacg gtgaatatcg
    10621 cactcgatct ggcgaagctt gaaaaagcca gacccgcgga acaaaccgct cccgtcaagt
    10681 aatatcaatc aggcacaaga aattgtgcct gattttttaa cagcggcaag atgccgtaaa
    10741 tcagatgcta caaaatgtaa agttgtgtct ttctggtgag ttacgcacta tccagacttg
    10801 aaaatagtcg cgtaacccat acgatgtggg tatcgcatat tgcgttttgt taaactgagg
    10861 taaaaagaaa attatgatgc gaatcgcgct cttcctgcta acgaacctgg ccgtaatggt
    10921 cgttttcggg ctggtactga gcctgacagg gatacagtcg agcagcgttc aggggctgat
    10981 gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt tcgcttctga tgtccaaatg
    11041 gatggcatta cgatctgttg gcggggaagt gatcgagcaa ccgcgtaacg aaagggaacg
    11101 ttggctggtc aatactgtag caacccaggc tcgtcaggcg gggatcgcta tgccgcaagt
    11161 ggctatctac catgcgccgg acatcaacgc ttttgcaacc ggtgcgcgcc gtgatgcctc
    11221 tctggttgct gtcagcaccg gtttgctgca gaacatgagc ccggatgaag ccgaggcggt
    11281 aattgctcac gaaatcagcc acatcgccaa tggtgatatg gtcaccatga cgctgattca
    11341 gggcgtggtg aacaccttcg ttatctttat ttcccgtatt ctggcgcagc ttgccgcggg
    11401 ttttatgggc ggaaatcgtg atgaaggtga agagagcaac ggcaacccgc tgatctactt
    11461 tgcggttgca acggttctgg aactggtgtt tggtattctg gcgagcatta tcaccatgtg
    11521 gttctcgcgt catcgtgaat tccatgctga tgccggttcg gcaaaactgg ttggtcgcga
    11581 gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat gaaccgcaag aagcaaccag
    11641 catgatggct ttctgcatta acggtaagtc gaaatcgctc agtgagttgt tcatgactca
    11701 cccgccgctg gataaacgta ttgaagctct gcgtacgggt gaatacctga agtaatatga
    11761 agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt ttatgccctg gctcgtggct
    11821 gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc gcagccatca
    11881 gcgagacgtg cgtaccatta tcaccaaact gatttagcat cagcgccacc agcgccgcgc
    11941 cactactctg acccagtaga cgagccgttc ctaacatgcc actggcccca ccgctacgtt
    12001 cgcgcggagc ggaggtaata atggtgtggt tattgggtga ctggaataag ccaaatccag
    12061 cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg ggcagcagaa
    12121 ccagggaaaa aagccccgca gccatgatga acaaccctaa agcacccagc aatcctgcat
    12181 gtacgcgttc aatcaaatag cctgccagcg gagccatcac catcgttgct aacggccacg
    12241 gtgtcagaag taaacctgtt tcgacttcac tacgcccgag cacggtttgc aggtaaaagg
    12301 gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg caaatagaaa
    12361 gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga agagaaagct
    12421 ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca gcaattaacg
    12481 tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg aaggttaacg
    12541 cgttcatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa ccattgggtg
    12601 gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg attaaaaata
    12661 accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg gcagcagaag
    12721 agacggcaac aataaacgag tttatgccca tccctctccc cagaaaacgt tgtggataga
    12781 tcaggcggat aagtgcggta ttaacgctca tcaacgctgc accgccgaaa ccttgtatga
    12841 cacgcgcaag ggtgagcatt tgcagcgaat cagaaagggc gcagaacagt gaagacaaca
    12901 gaaaaacgac cagaccgcat ttataaatac gtcgatagcc aaacatatcg cccagaaacg
    12961 aaaacgagag cagggagatg acaatggcga tttgataggc gttcactacc cagatggaac
    13021 tggctggtgt ggcatgaagg tccgtggcga ttgttggcag ggcgacgttg gcgattgcgc
    13081 cgtcaaggac agccatcgaa ataccaatca caatggttaa tatcgcaccg tatcgctggg
    13141 gcaatggcag gccgtcggcc tgaacttttg gcataggaat tttatatctt tggtgaataa
    13201 tcaacagggg tatgattcta gcactgatat tcacggtgga tgtcgcagaa ttgtaactaa
    13261 ccggcaaaag atggattgcg accaccctgg tgcgaaatta taataaaaaa cagttctgat
    13321 ttttataaaa cactcgcaat gaggtgataa atggctaacg cagatctgga taaacagcct
    13381 gattctgtat cttccgtgct aaaagttttt ggcattttgc aggcgctggg tgaagagcgc
    13441 gaaataggga taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac cgtttatcgc
    13501 tttttacaga ccatgaaaac cttaggttat gtggcgcagg aaggggagtc ggagaaatat
    13561 tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt cgatttaatt
    13621 cgtagcgcag atatccagat gcgtgagatc tcccgcctga ccaaagaaac tatccacctc
    13681 ggcgcactgg acgaagacag tattgtttac attcacaaaa ttgactctat gtacaatttg
    13741 cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca gcaccgcgat tggtaaggta
    13801 ctgctggcat ggcgcgatcg cgatgaagtg aagcaaattc ttgagggcgt ggagtataaa
    13861 cgcagtaccg agcggaccat caccagtaca gaagcgttat tacccgttct ggaccaggtg
    13921 cgcgagcagg ggtatggcga agataacgaa gagcaggaag aagggctgcg atgcattgcg
    13981 gtaccggtat ttgatcgctt tggcgtggtc attgccggtt tgagcatctc cttcccgacg
    14041 ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac cgcagcgcgc
    14101 aaaatttctg cccaaatggg ttatcacgac tatccgttct gatgagagta agaacctgtc
    14161 ggaatatcaa acagacaggt tctttattta gcatgagaaa aataaagttg aaggtgcgtt
    14221 atattaaacg cgcttgctat aagaatattt tactcatgag tgagaatctg gttatttatt
    14281 acccttaacc attatcgacc acgatattgc ttttacgtaa cagtgggcaa tctgttatcc
    14341 ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg gcagtcagat
    14401 aggtacattg caaacctaat cctgcggcat tctctttgct tccaatcaaa acgccatatc
    14461 cgctgagtaa taatcctatc cataccagtg ctatcagcat aactgtgcga atgatgaatc
    14521 gcattacaac ctcttctctt tttatgttcg cttaatcgta gcggcaatat gcgctgaagc
    14581 aagctactca ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt taactaacgc
    14641 atgctagttt aatgacataa ggtaggtgaa gcggagattg gagtgaaaaa gtttcgatgg
    14701 gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt attcaacatg
    14761 atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca gtttatcccg
    14821 tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt agaatagcgc
    14881 ctctactcag aggtggtgaa atgaccgaag ttgtaaattc aggcgtgatg aacattgcgt
    14941 ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt tttatcaatc
    15001 gtgccagttc acggactaac gaacagattg aactgcttga ggcgttgctg gatcagcaaa
    15061 aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata
    15121 aaaagaccat tgagagtcaa aaatcggttg atgacgaaga tattattcgc ctggtcgccg
    15181 aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact caattcatcc
    15241 tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc tgacttcaaa
    15301 taattatcat attatttatt tgcgtaacgg gatatcagaa aaagatattt tcctcattaa
    15361 tttattagga tgtttacatc agatttgtga ttaagcgtgg tattatttat tagcgaaacg
    15421 tttctctctt gatttttttg ctcactcatc aatttttctt attttaaatt tacaatccct
    15481 ttggggattg acttctcttt agggtaattg atagccgtta actgactgtt ttatgagaaa
    15541 aagtgatata actttttatt cattgcatag cagaaaatgt gatattgcac gcactatgta
    15601 ataacttctc ccactggcct ggaacaactg aacttattga actatgttag aaactacgcc
    15661 agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg cgcgtattcc
    15721 gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac
    15781 atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taaaagattt cgggttaaac
    15841 gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt aaacacgttg
    15901 acgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt aatgtgtcag
    15961 ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt caaatggcaa
    16021 agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc attactccgg
    16081 ctgatggcag caaagatgtg ttcgtacact tctccgctat ccagggtaat ggcttcaaaa
    16141 ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa ggtccggcag
    16201 ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat acgcttcaat
    16261 ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt cgctcctggc
    16321 gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag tatcctgccg
    16381 gagttgtcat gtcgttttcc tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca
    16441 gctatatctg tccccaacga catcagtttg atatggcgaa agaagggtat gtcaatctgc
    16501 tgcccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg atgcaagcac
    16561 gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt gtcgcccaac
    16621 tgcgggaacg gcttgatgaa aaggccacgg cggtgctgga tattggctgt ggtgaagggt
    16681 attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggc ctggatgtct
    16741 cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactctt tgtgtcgctt
    16801 ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt atttacgcgc
    16861 cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc attactgcca
    16921 cgccggaacc gcgacatttg atggagctga aggggctgat ttacaatgaa gtacatcttc
    16981 atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg gagttgtgtt
    17041 atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg ccgtttgcct
    17101 ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc gactgccaga
    17161 cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc gtccagagga
    17221 tctggacgcc gatgccgatc agcaccagcc cgccgagaat ttccgctttt ttcccaataa
    17281 ttgagccgat aaagcgacca accatcatcc ctaatgtcga cataatcaag gttgcacaac
    17341 caatggccaa tgcggtcgcg ataatgttga cctgaaggaa agcaagacca acacccacag
    17401 ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa ccgtgccgac
    17461 ggtgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc attcgcccgc
    17521 cgaggaatat cagcagcaca aacgcaatcc agtgattcca ttcaaggaca aaccggctgg
    17581 ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca ccaaaaataa
    17641 ggccggttcg caatgcttca gaaaatttcg gtttatggag gggggcacct ttaccgattg
    17701 atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca gtgatattca
    17761 tgacaacgtc ctgaccgggg tatccataaa acacatcatc gcccccagca aacatgcata
    17821 catgaacaac aagccgcgat gggttttcgc gttcatgcag gcatagcgat tgatgtgcat
    17881 atggtctcgc ctgactgaaa gtcttcagtc cgtacgtgcc acgttttgca acgagtatgt
    17941 tgacacgtac atttccggaa tctggaaatc ggctactccc caaatgaggg cgcaacctta
    18001 acatattttg agaatataaa acaactgttg gaatatatta tttcattgct ttattgataa
    18061 cgattttcat ttatgtttat atatcaatga aacgttaaaa taaataacaa ataagggaag
    18121 agataatata gcctaagcta tgtctgatta ttaaaaatac actaaacata gctttggcta
    18181 aattcataag ttattgagtt gata
//
LOCUS       DADLSA010000079        17697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.079, whole genome shotgun sequence.
ACCESSION   DADLSA010000079 DADLSA010000000
VERSION     DADLSA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.079"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            1..204
                     /locus_tag="JN691_002580"
                     /pseudo
     CDS             1..204
                     /locus_tag="JN691_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="ethanolamine utilization protein; involved in the
                     degredation of ethanolamine; frameshifted; incomplete;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutK"
     gene            250..1302
                     /gene="eutR"
                     /locus_tag="JN691_002581"
     CDS             250..1302
                     /gene="eutR"
                     /locus_tag="JN691_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HAY7243996.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(1308..2207)
                     /gene="hemF"
                     /locus_tag="JN691_002582"
     CDS             complement(1308..2207)
                     /gene="hemF"
                     /locus_tag="JN691_002582"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAY7243997.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(2211..3080)
                     /gene="amiA"
                     /locus_tag="JN691_002583"
     CDS             complement(2211..3080)
                     /gene="amiA"
                     /locus_tag="JN691_002583"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAY7243998.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVINYF
                     HWFDNQKAHSKKR"
     gene            3294..3719
                     /locus_tag="JN691_002584"
     CDS             3294..3719
                     /locus_tag="JN691_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase YpeA"
                     /protein_id="HAY7243999.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            3706..4155
                     /locus_tag="JN691_002585"
     CDS             3706..4155
                     /locus_tag="JN691_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAY7244000.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            4216..4791
                     /locus_tag="JN691_002586"
     CDS             4216..4791
                     /locus_tag="JN691_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAY7244001.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            4887..5786
                     /gene="yfeX"
                     /locus_tag="JN691_002587"
     CDS             4887..5786
                     /gene="yfeX"
                     /locus_tag="JN691_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAY7244002.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVTVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(5966..7390)
                     /gene="murP"
                     /locus_tag="JN691_002588"
     CDS             complement(5966..7390)
                     /gene="murP"
                     /locus_tag="JN691_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAY7244003.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(7394..8290)
                     /gene="murQ"
                     /locus_tag="JN691_002589"
     CDS             complement(7394..8290)
                     /gene="murQ"
                     /locus_tag="JN691_002589"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAY7244004.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTELMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            8454..9311
                     /gene="murR"
                     /locus_tag="JN691_002590"
     CDS             8454..9311
                     /gene="murR"
                     /locus_tag="JN691_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HAY7244005.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVASLKMIQR
                     SSELTQRLK"
     gene            9440..10231
                     /gene="ucpA"
                     /locus_tag="JN691_002591"
     CDS             9440..10231
                     /gene="ucpA"
                     /locus_tag="JN691_002591"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAY7244006.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            10535..11551
                     /locus_tag="JN691_002592"
     CDS             10535..11551
                     /locus_tag="JN691_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7244007.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            11551..12384
                     /gene="cysT"
                     /locus_tag="JN691_002593"
     CDS             11551..12384
                     /gene="cysT"
                     /locus_tag="JN691_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAY7244008.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYIWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGLEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            12384..13259
                     /gene="cysW"
                     /locus_tag="JN691_002594"
     CDS             12384..13259
                     /gene="cysW"
                     /locus_tag="JN691_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAY7244009.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            13249..14346
                     /gene="cysA"
                     /locus_tag="JN691_002595"
     CDS             13249..14346
                     /gene="cysA"
                     /locus_tag="JN691_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAY7244010.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            14480..15391
                     /gene="cysM"
                     /locus_tag="JN691_002596"
     CDS             14480..15391
                     /gene="cysM"
                     /locus_tag="JN691_002596"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAY7244011.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMLELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(15595..16318)
                     /locus_tag="JN691_002597"
                     /pseudo
     CDS             complement(15595..16318)
                     /locus_tag="JN691_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(16351..16725)
                     /locus_tag="JN691_002598"
     CDS             complement(16351..16725)
                     /locus_tag="JN691_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAY7244012.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQMDKTVPAEGN"
     gene            16830..17681
                     /gene="pdxK"
                     /locus_tag="JN691_002599"
                     /gene_synonym="thiJ"
     CDS             16830..17681
                     /gene="pdxK"
                     /locus_tag="JN691_002599"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAY7244013.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHEIDELI
                     LPPLAEA"
BASE COUNT         4160 a         4411 c         4890 g         4236 t
ORIGIN      
        1 ggaacccgac gcgccagtta tcgtcgcgga atctgctgac gagttgttgg cgctgttaac
       61 atcagtatgt cagggaatga cggcaggaga agtggccgcc cactttggct ggccgctgga
      121 aaagccagaa atgcgctcga acagctcttt tctgccggga cgttacgtaa acgcagtagt
      181 cgttatcgtc tcaagcccca ttaacctgtc ggaggtgccg ggtgtcctac aacacccggc
      241 attaacatca tgaaaaagac ccgtacagcc aatttgcacc atctttatca tgaaccctta
      301 cccgaaaacc tgaagctcac gccgaaggtc gaagtggata atgttcatca acgacagaca
      361 acggatgtct atgaacatgc tttgacgatt accgcctggc agcagattta cgatcagctg
      421 catccgggca agtttcatgg tgaatttacg gaaattctac tcgatgatat tcaggttttt
      481 cgtgaataca ccggtctggc gctgcgccag tcgtgcctgg tctggccgaa ctcgttctgg
      541 tttggcattc cggcgacgcg cggtgagcag ggatttatcg gttcgcaatg tctgggaagc
      601 gcagaaatag cgacgcgccc cggtggaacc gagtttgaat taagtacgcc ggacgattat
      661 acgatcctgg gcgtagtgct gtctgaagat gtcatcaccc gtcaggctaa ctttttgcac
      721 aatccggatc gggtattaca tatgctgcgt agccagtcgg cgctggaagt gaaagagcag
      781 cataaagccg cgctgtgggg ctttgtccaa caggcgctgg cgacgttttg cgagaacccg
      841 gaaaatctcc atcagcctgc agtgcgaaaa gtgctggggg ataatttgct aatggcgatg
      901 ggggcgatgc tggaagaagc gcaaccaatg gtgacggcgg aaagcatcag tcatcagagt
      961 taccgtcggt tgctttcccg cgcccgtgaa tatgtgctgg aaaacatgtc cgaaccggtg
     1021 acggtgctgg atttgtgtaa tcaactgcat gttagtcgcc gcacgctaca aaacgcgttt
     1081 cacgctattt taggcattgg cccaaacgcg tggctgaaac gcattcgcct gaacgccgta
     1141 cgccgcgaac tgataagccc gtggtcgcaa agcacaacgg taaaagacgc cgccatgcag
     1201 tggggattct ggcatctggg gcaatttgcc acggattacc agcagctgtt tgccgagaag
     1261 ccgtcgttga cgttgcatca gcggatgcgg gaatgggggt gagggagtta cacccaatcc
     1321 ctgaccttaa taaactcact taacgccgct tctgggctgc catcttttgg ctgataatca
     1381 tactcccagc gtaccagtgg cggcattgac atcaggatcg actcggtgcg cccgccagtt
     1441 tgcaggccaa acagcgtgcc gcgatcccag accagattga actcgacata acgaccgcga
     1501 cggtagagct gaaaattgcg ctcgcgctcg ccgtaggcca tcgctttacg tcgctctaca
     1561 attggtaaat aagcgtcggt gtagcctttg cctaccgcct gcataaaggc aaaacagtgg
     1621 tcgaaatctg gcgtgttcag atcatcaaag aacagcccgc caatgccgcg ctgttcgttg
     1681 cgatgtttga ggtagaagta atcgtcgcac cactttttgt aacggggata aacgtcttca
     1741 ccaaatggca ggcacaggtc acgggcggtg cggtgccagt gaatggcgtc ttcttcaaaa
     1801 ccatagaaag gggttaaatc gaagccgccg ccaaaccacc aaacgggctc tgcacccggt
     1861 ttttcggcaa taaaaaaacg cacattcgcg tggctggtgg gaacatacgg gttatgcgga
     1921 tgcactacca gtgaaacgcc catcgcctcg aaactgcgcc cggcaagttc cgggcgatga
     1981 gcggtggcgg aagcaggcat cgcctcaccg tggacgtgag aaaagttgac gcccgcctgt
     2041 tcgaaaacac caccattacg caacacccga ctgcgcccgc cgccgccagc ttcgcgctgc
     2101 caactatctt cgacaaattc tgcgccatcg acggcggtca gctgctgaca aatcgtatcc
     2161 tgaaggttga gcagaaactg tttaacctgg tgtgcgtcgg gtttcataac ttatcgcttt
     2221 ttcgaatgtg ctttctggtt gtcgaaccag tggaaataat tgatcacgcc ttcagcaatc
     2281 gctgtggcga ttttctgacg aaacgccgcc gtgcctaaca gccgctcttc ttccgggttg
     2341 gtgataaacg aggtttccac cagcaccgaa ggaaccgacg gcgatttcaa caccacaaac
     2401 gcggcttgtt cggtgttgcg gctgtgcagt ttatgcaccg gcttaatctt tttcagaata
     2461 tgcgagccaa gcgtcaggct gtttttaata gtatcggttt gcaccagatc aaacagtact
     2521 tgctgcaata ggtgatcctt gtcagtcgcc tttttaccgg caacttcatc ggcgcggttt
     2581 tcgcgttcag acaggtattt cgccattgcg ctacttgccc cacggttaga gagggcaaat
     2641 accgaagcac cggcagcttt cgggttggta aagccatcgg catgaattga cataaacaga
     2701 tctgcgccat gtttatgggc gatttcaacg cgatcgtaaa gtgggataaa cgtatcgcca
     2761 gaacgcgtta aacgcgcatc aatcccatga ttacgcaaaa tggaacggac gtttttagca
     2821 atcgccagca ccacatgttt ttctttcgaa ccgttgcgtc cgatcgctcc ggtatcaatt
     2881 ccgccgtggc ctggatcgag aacaacgaca cgtttgccgc cagatttttt ggctttcggc
     2941 ttgctgtgtc cgttgctggt ttttaaaggt tcgtctttgg cgatggcttg cgacattcct
     3001 gacaacgtca gggcagctaa accggctttc agcacctggc ggcgcgaagt gagtgttttt
     3061 agtggtttaa aagtgctcat acggcctgag ttgtaataat aaagttccag atgttatatc
     3121 gtatcgcgta atccgttacg acggtttgct gttgagaatt gttttacttt tcatttcaat
     3181 agttgacaac tccccattat gccacttttt caaccgattt tcgtcagata ttggctaatt
     3241 cgaccgttca cgccataatc agccttttaa cccgcaaaaa ggtaacgaat accatggaga
     3301 tacgcgtatt tcgccaggaa gatttcgaag aggtcatcac cctttgggag cgttgcgact
     3361 tgctgcgtcc gtggaacgat ccggaaatgg acatcgagcg taagatgaac catgacgtca
     3421 gtttgttttt ggtcgctgag gtaaacggtg aagtggtcgg aacggtgatg ggcggttatg
     3481 acgggcatcg cgggtctgct tattatcttg gcgtgcatcc agagtttcgt gggcgtggaa
     3541 ttgccaatgc gttgcttaac cggctggaga aaaagctgat tgctcgcggc tgcccgaaaa
     3601 ttcagatcaa cgtgccggaa gataacgaca tggtgctcgg aatgtatgaa cgcctgggat
     3661 atgaacacgc cgacgtgctg agtctgggta agcgtttgat tgaagatgaa gagtactgag
     3721 ttccatcctg tccattatga tgcgcacgga cgcctgcgtt tacccttgct cttctggctt
     3781 gtgctattgc ttcaggcgcg aacctgggtg ctgtttgtca ttgccggtgc gtcgcgcgag
     3841 cagggtactg cgctgctaaa tctgttttat cccgatcacg ataatttctg gctggggctg
     3901 attcccggta ttccggcggt gctggcgttt ttgttgagcg gtcggcgggc ttcgtttcct
     3961 cgcacctggc atgtgctcta ttttctgttg ctgttggcgc aggtggtttt actttgctgg
     4021 caaccgtggc tgtggctgaa cggtgaatcc gttagcggta tcggtctggc gctggtagtg
     4081 gcggatattg tggcgttaat ctggctgctg accaatcgac gtttacgcgc ttgttttaat
     4141 gaagaaaaag aataacggca ctttttggtg aatttgcact ccaagcaacg ttattgaata
     4201 accaaaggaa gtgacatgaa atcgctgcgt ttaatgttat gcgctatgcc gttgatgctg
     4261 accggctgtt ccacgatgtc gtcagttaac tggtctgccg ctaacccgtg gaactggttt
     4321 ggatcgtcca ccaaagtgag cgagcagggc gtgggtgaat taacggcgtc cacaccactg
     4381 caagaacaag ccattgctga tgcgcttgat ggcgattatc gcctgcgcag cggaatgaaa
     4441 accgcgaacg gcaacgtggt gcgctttttt gaagtgatga aaggcgacaa cgtggcgatg
     4501 gtgattaacg gcgatcaagg cacgatcagt cgcattgacg tgctggatag cgatattcct
     4561 gctgacaccg gtgttaaaat cggtacaccg tttagcgacc tttacagcaa agcatttggc
     4621 aattgccaaa aagctgatgg tgatgataat cgtgccgtcg aatgtaaagc cgaaggcagt
     4681 caacatatta gctaccagtt cagcggggaa tggcgtggtc ctgaagggtt aatgccttcg
     4741 gacgataccc tgaaaaactg gaaagtcagt aaaattatct ggcggcgtta atttgcgtct
     4801 gaacaaaccg ccgtcgcgaa aaaacacgta aaatagcgct caacaatgcc acggattgcg
     4861 tggcattctt attttcagga ggaacaatgt ctcaggttca gagtggcatt ttgccagaac
     4921 attgccgcgc ggcgatttgg atcgaagcca acgtgaaagg ggaagttgac gccctgcgtg
     4981 cggccagtaa aacatttgcc gacaaactgg caacttttga agcgaaattc ccggacgcgc
     5041 atcttggtgc ggtggttgcc tttggtaaca acacctggcg cgctctgagc ggcggcgttg
     5101 gggcagaaga gctgaaagat tttccgggct acggtaaagg ccttgcgccg acgacccagt
     5161 tcgatgtgtt gatccacatt ctttctctgc gtcacgacgt aaacttctct gtcgctcagg
     5221 cggcgatgga agcctttggt gactgcattg aagtgaaaga agagatccac ggcttccgtt
     5281 gggtggaaga gcgtgacctg agcggctttg ttgacggtac agaaaacccg gcgggtgaag
     5341 agacgcgccg cgaagtgacg gttatcaaag acggcgtgga tgccggcggc agctatgtgt
     5401 ttgtgcagcg ttgggagcac aacctgaagc agctcaaccg gatgagcgtt cacgatcagg
     5461 agatgatgat cgggcgcacc aaagaggcca acgaagagat tgacggcgac gaacgtccgg
     5521 aaacctctca cctcacccgc gttgatctga aagaagatgg caaagggctg aagattgttc
     5581 gccagagcct gccgtacggc accgccagtg gcactcacgg tctgtacttc tgcgcctact
     5641 gcgcgcgtct gcataacatt gagcagcaac tgctgagcat gtttggcgat accgatggca
     5701 aacgtgatgc gatgttgcgt ttcaccaaac cggtaaccgg cggctattat ttcgcaccgt
     5761 cgctggacaa gttgatggcg ctgtaagcgt ttctgattca ttgattaaag gccaggcagt
     5821 aacattactg actggccttt ttattactgc tttaacgccg catatacttg atcaactacc
     5881 ttctgtcaga acagggtatt ttgcggcgct gacgctacaa ctaacagcca gcagagaaag
     5941 atagagcatt gtccgtttca taatatcagt ccagattgac gttacggcag ccaaagagcg
     6001 tggtgaaaat aaacccgcaa acccatgcca ccagaatccc gccagcataa accgccattg
     6061 ccggaaggat gccttgtgcg gaagtcatca gcggcagcgc caccagacca gacggcccaa
     6121 aggcgctgtt taagcccatc ggcagacccc accaggctat caatccgata aacaaaccgc
     6181 ccgccgcgcc gcctaaacag gcggtaacaa acggcttcat gcggggcagg gtgacaccgt
     6241 aaatcagcgg ttcaccaacg cccagcaggc cgggaataat cgccccgcgt acctgactgc
     6301 gtagcgcact gtgcggttgc gcccgccagt agagtgccag cgccgcgccc acctggcccg
     6361 cgcctgccat tgaaaggatg ggaaataagc tgttgaatcc ctggctgtcc attaacgcga
     6421 ggtaaacagg aataaagccc tgatgcacgc caaataccac ggcgatcagg aacagcccgg
     6481 ctaaaaccgc acaaccgaac ggattactgt tcaggtgcat aaacagccat gacatacctt
     6541 cgaataacca gccacccagc ggcataatga tcaggtacgc aagcgtagcg gtgatcagca
     6601 gggtgattaa cgaggtcagc agcatgtcga gatcgtccgg cataaagcgg cgcaccatgc
     6661 cttcaatgcg ggcgcaggcc cacgcggcaa tcagcacgcc gataatattg ccgcgcggat
     6721 cgatgggcag accaaagaaa tcgtgaaaac cggcgtagta accggtggtt gcagcggggt
     6781 tataaccgag caaaaagagt gcggcaataa ttgcgccatt gacgcccgtg ccgccaaatg
     6841 cctgggcggc gttatagccc accagaatca ccaggaaggt gaacaaacct ttgctgaaca
     6901 ccttcataaa attcagcgcg tcgggtagcg tgccctgagc atctgccgga acgtgcatca
     6961 ccgtggcaat taacgtcgct atccccagca gcagaccggc ggcaataaaa ccggggatca
     7021 gcggcgtgaa gatggtggcg aatttggcga gaaattgttg cacgccggag gtttgtttgg
     7081 cttttaactg acgtttgttc tgggcggcga tttctgcggc atcctgtacc ggtgcctcgc
     7141 ccagcagctc gctcatcgct tttgcggcac gatgcgcttt accaggccca aaaacgacct
     7201 gcacctggtc gctggtcaaa atgacgccct tcacgccttc cagcgttttg atattgggat
     7261 caaccagtga actgtcatgt acacccagac gcaggcgcgt catacagtta ccacaactgg
     7321 cgatatttcc cggtccgccg acacgggtaa gaatggtgtt cagaagttca ctgctgatct
     7381 ctttggccat gggttattcc ttgtctaaaa cctgacgaat aaagccgccg tgttgatcca
     7441 ggcgtttttt agcttctgcg gcatcgagat ttttcagcac catcacaatg gccgttttac
     7501 agttgcgctc gcaggcaatt aacgccgctt ccgcttgttc ggcattacat ccggtggcgt
     7561 ttttaacgat attgacctgg cgcacatgca gtttttcgtt ggtggcgacc acatcgacca
     7621 tcaggttgcc gaacactttg ccggatttaa tcatcagctc ggtggaaagc atattgagca
     7681 ccagtttctg cgctgtacct gctttcatcc gcgaagaacc ggtaacaact tcggcaccta
     7741 caatcggtgt aatggcaaac tcagcggtgg ttgaaacggc gctccccgga ttacaggaaa
     7801 ttcccactgt gcggcagccg agctggcgtg cgtattccag tccggcaatc acatacggcg
     7861 tgcgaccgct ggcagcaatg ccaaccacca catcctgtgc cgttaaatta atatttttca
     7921 gatcgttaac accaccttcc cggctatctt cagcgccttc caccgcgtgc tgaatggcat
     7981 attcgcctcc agcaatcaaa ccaaccacca gacccggttt cacgccgtag gtgggcggac
     8041 attcgctggc atccagaatc cccagacgac cggatgttcc cgcaccgaga taaatcagac
     8101 gaccgccgcc gctgacctgg gcgtggataa catcgatcgc cgcggcgata tccggcagga
     8161 cgcgctcaac ggcaagcggt acggttttat cttcatcgtt gataatccgg cacatttcca
     8221 gcgtcgatac gcggtcaatt tcagccgagg cggtgttcga gccttcagta atcatctttt
     8281 ctagttgcat cggtggtgac cttactattt acgatcaaat aattgcagat gtatggaatc
     8341 atatatcccg aattgacaga cttaaaggaa tcattgagtc tacactcgca gagtgtgaag
     8401 agcgtcataa gaacttaagc ggaatttttg ctgccagccc aggagagtgg aagatgttgt
     8461 acctgacgaa aatacgcaat gcggaaagtg aatttaccgg gaacgagcaa aaaattgctg
     8521 actttctgcg ggccagagtg agcgagctga aatccgtctc ttcacggcag atggcgaaac
     8581 agctcggtat cagccagtcg agcattgtga aatttgccca gaagctcggc gcgcaaggat
     8641 ttactgaatt gcgtatggcg ttaattggcg aatacagtgc cagccgggag aaaacgaatg
     8701 ccacggcgct gcacctgcac agttcgatta ccagtgatga ttcgctggaa gtgattgccc
     8761 gcaaactgaa tcgcgaaaaa gagctggcgc tggagcaaac ctgtgcgttg ttcgactacg
     8821 cgcggctgca aaaaatcatt gaggtcatca gtaaagcacc gtttatccag ataacgggcc
     8881 tgggcggatc ggcgctggtg gggcgcgatc tgtcgttcaa actgatgaaa attggctatc
     8941 gcgtggcctg cgaagccgac acgcacgtac aagccaccgt ttcccaagcg ttgaaaaaag
     9001 gcgatgtaca aatcgctatc tcttacagcg gcagtaaaaa agagattgtc ttgtgcgcag
     9061 aagcggcacg aaagcagggg gcaacggtga ttgccattac ttctctggct gactctccgc
     9121 tgcggcggct ggcgcatttc acgctcgata ccgtttccgg tgaaaccgaa tggcgtagct
     9181 cgtcgatgtc tacccgcacg gcgcaaaatt ctgttaccga tttactgttt gttggtcttg
     9241 tgcaactcaa tgatgtggcg tcattgaaaa tgattcagcg cagcagtgag ctaacacaac
     9301 gcctgaaatg atgcatagag cagcgactgg attgagattt tcctgaatta gtgagctgat
     9361 ccgcagcaat attttgttta tcctgtattt tcagagggaa tggagtgtaa cgctctgtat
     9421 taacaaggag agcattaaaa tgggtaaact cacgggcaag acagcactga ttacgggcgc
     9481 attgcaggga attggcgaag gaattgccag aacttttgca cgtcatggcg cgaacctaat
     9541 cttgctggat atctcccctg agatcgaaaa gctagcggac gaactgtgtg gtcgtggtca
     9601 tcgctgtacg gcggttgtcg ccgatgtgcg tgacccggcg tcggtagccg cagctatcaa
     9661 acgcgcgaag gaaaaagaag ggcgcattga tatcctggtg aataacgcag gcgtttgtcg
     9721 tctgggcagt ttcctcgata tgagcgatga agatcgcgat ttccatattg atatcaatat
     9781 taaaggcgta tggaacgtca cgaaggcggt gctgccggag atgattgcgc gcaaagatgg
     9841 tcgcattgtg atgatgtctt cagtcactgg tgatatggtg gccgatcctg gcgaaacggc
     9901 gtatgcctta acgaaagcgg cgattgttgg cctgactaaa tcgctggcgg tggagtacgc
     9961 gcaatccggt attcgcgtta acgccatctg cccgggatac gtccgcacgc caatggcgga
    10021 aagcattgcc cgccagtcga acccggaaga tccagaatcg gtgctgactg aaatggcaaa
    10081 agcaatcccg ctgtgtcgcc tcgccgatcc gctggaagtc ggcgaactgg cggccttcct
    10141 cgcatcggat gaatccagct atttaaccgg tacacagaat gtgattgatg gcggcagcac
    10201 actgccggag acggttagcg tcggtatctg attcacctct gtttcctccc tgcatgtgtg
    10261 gggaggattt cgtcttgaac taagttcacc aggctatttc atttgtcatt ttggccccgg
    10321 gcagtgctcg aaatcctcac gtactatgtg tacgctccgg tttctccgcg ctgtccgtgt
    10381 ccaaactgcc tgcaacaatt acgcctgttg aaccaagttc ttattccctt tttaacttcc
    10441 aaatcaccaa acggtatata aaaccgttac tcctttcacg tccgttataa atatgatggc
    10501 tattagaaag tcattaaatt tataagggtg cgcaatggcc gttaacttac tgaaaaagaa
    10561 ctcactcgcg ctggtcgctt ctctgctgct ggcgggccat gtacaggcaa cggaactgct
    10621 gaacagttct tatgacgtct cccgcgagct gtttgccgcc ctgaatccgc cgtttgaaca
    10681 gcaatgggca aaagataacg gcggcgacaa actgacgata aaacaatctc atgccgggtc
    10741 atcaaaacag gcgctggcga ttttgcaggg cttaaaagca gacgttgtca cttataacca
    10801 ggtgaccgac gtacaaatcc tgcacgacaa aggcaagctg atcccggctg actggcagtc
    10861 gcgcctgccg aataatagct cgccgttcta ctccaccatg ggcttcctgg tgcgtaaggg
    10921 caacccgaag aatatccacg actggaacga cctggtgcgc tccgacgtga agctgatttt
    10981 cccgaacccg aaaacgtcgg gtaacgcgcg ttatacctat ctggcggcat ggggcgcagc
    11041 ggataaagct gacggtggcg ataaagccaa aaccgaacag tttatgaccc agttcctgaa
    11101 aaacgttgaa gtgttcgata ccggcggtcg tggcgcaacc accacttttg ccgagcgtgg
    11161 cttgggcgat gtgctgatta gctttgaatc ggaagtgaac aacatccgta aacagtatga
    11221 agcacagggc tttgaagtgg tgatcccgaa aaccaatatt ctggcggaat tcccggtggc
    11281 gtgggtcgat aaaaatgtgc aggccaacgg tacggaaaaa gccgcaaaag cctacctgaa
    11341 ctggctctac agcccacagg cgcagaccat catcaccgac tattactacc gtgtgaataa
    11401 cccggaagtc atggacaaac tgaaagataa attcccgcag accgaactgt tccgcgtgga
    11461 agacaaattt ggctcatggc cggaagtgat gaaaacccac ttcaccagcg gcggcgagtt
    11521 agacaagctg ttagcggcgg ggcgtaagta atgtttgcag tttcttcaag acgcgtgctg
    11581 ccgggcttta ctttaagcct tggcaccagt ctgctgtttg tgtgcctgat attgctgctg
    11641 ccgctctctg cgctggtgat gcagctttct gagatgagct gggcgcagta ctgggaggtg
    11701 atcaccaacc cgcaggtagt cgcggcctac aaagtgacgc tgctgtcggc gtttgtggca
    11761 tcgattttta acggcgtttt cggtctgctg atggcgtgga ttttaacacg ttatcgcttt
    11821 ccgggccgca cgctgcttga tgcgctgatg gatttaccct ttgcgctgcc aacggcagtc
    11881 gccggtttaa cgctggcctc gctcttttcc gtaaacggtt tttacggtga atggctggcg
    11941 aagtttgata tcaaagtcac ctatatctgg cttggtattg cggtggcgat ggcctttacc
    12001 agcattccgt ttgtggtgcg taccgtgcag ccggtgctgg aagagttagg cctggaatat
    12061 gaagaagcgg cggaaacgct tggtgcaacg cgctggcaga gtttctgcaa agtggtgctg
    12121 ccggagcttt ctccggcgct ggtggcgggc gtggcgctgt cgtttacccg tagtcttggt
    12181 gaatttggcg cggtgatttt tatcgccgga aatatcgcgt ggaagacgga agtgacgtcg
    12241 ctgatgattt ttgtgcgctt acaggagttt gattacccgg cagcgagcgc gattgcttcg
    12301 gtgatcctcg cggcatctct gctgctgctg ttctcaatta acactctgca aagtcgcttt
    12361 ggtcggcgtg tggtaggtca ttaatggcgg aagttaccca attgaagcgt tatgacgcgc
    12421 gcccgattaa ctggggcaaa tggtttctga ttggcatcgg gatgctggtt tcggcgttca
    12481 tcctgctggt gccgatgatt tacatcttcg tgcaggcatt cagcaagggg ctgatgccgg
    12541 ttttacagaa tctggccgat ccggacatgc tgcacgccat ctggctgacg gtgatgatcg
    12601 cgctgattgc cgtaccggta aacctggtgt tcggcattct gctggcctgg ctggtgacgc
    12661 gctttaactt cccgggacgc cagttactgc tgacgctact ggacattccg tttgccgtat
    12721 cgccggtggt tgccggtctg gtgtatctgc tgttctacgg ctccaacggc ccgctcggcg
    12781 gctggctgga tgaacataat ctgcaaatta tgttctcctg gcccggaatg gtgctggtca
    12841 ccatctttgt gacgtgtccg tttgtggtgc gcgagctggt gccggtgatg ttaagccagg
    12901 gcagccagga agacgaagcg gcgattttgc ttggcgcgtc cggctggcag atgttccgtc
    12961 gcgtcacatt gccgaatatt cgctgggcgc tgctttacgg cgtggtgctg accaacgccc
    13021 gcgcgattgg cgagtttggt gcagtgtcgg tggtttccgg ctcgattcgc ggcgaaacac
    13081 tttcgctgcc gttacagatt gaattgttgg agcaggacta caacaccgtc ggctccttta
    13141 ccgccgccgc gctgttgacg ctgatggcga ttatcaccct gtttttaaag agtatgttgc
    13201 agtggcgcct ggagaatcag gaaaaacgcg cgcagcagga ggagcatcat gagcattgag
    13261 attgccaata tcaagaagtc ttttggtcgc acccaggtgc tgaacgatat ctcactggat
    13321 attccttctg gtcagatggt cgcgttgctg gggccgtccg gttccgggaa aaccacgctg
    13381 ctgcggatta tcgccgggct ggagcatcaa accagcgggc atattcgctt ccacggcacc
    13441 gacgtgagtc gcctgcacgc gcgcgatcgt aaagtcggtt tcgtgttcca gcattacgcg
    13501 ctgttccgcc atatgacggt attcgacaat atcacttttg gcctgacggt gctgccgcgt
    13561 cgcgagcgcc cgaatgccgc agccatcaaa gcgaaagtga caaaattgct ggaaatggtc
    13621 cagcttgcgc atctggcgga tcgttatccg gcgcagcttt ccggcggcca gaaacagcgt
    13681 gtggcgctgg cgcgcgcgct ggctgtagaa ccgcaaattc tgctgcttga tgaaccgttt
    13741 ggcgcgctgg atgcgcaggt gcgtaaagag ctgcgtcgct ggctgcgtca actgcatgaa
    13801 gagctgaaat tcaccagcgt gtttgtgacc cacgaccagg aagaggcgac cgaagtagcc
    13861 gatcgtgtag tggtgatgag tcaggggaat attgaacagg ctgatgcgcc gaatcaggta
    13921 tggcgcgaac cagcgacccg ttttgtactc gaatttatgg gcgaagtgaa ccgcctgcag
    13981 ggaaccattc gcggcgggca gttccatgtt ggcgcgcatc gctggccgct gggctacaca
    14041 cctgcgtatc agggtccggt ggatctcttc ctgcgcccgt gggaagtgga tatcagccgc
    14101 cgtaccagtc tcgattcgcc gctgccggta caggtactgg aagccagccc gaaaggtcac
    14161 tacacccaat tagtggtgca gccgctgggg tggtacaacg aaccgctgac ggtcgtgatg
    14221 catggcgacg atgccccgca gcgtggcgag cgtttattcg ttggtctgca acatgcgcgg
    14281 ctgtataacg gcgacgagcg tattgaaacc cgcgatgagg aacttgctct cgcacaaagc
    14341 gcctgatagg ttgagtgaat gttaaacgcc cggaggcgct tcccgcgatc cgggcttttt
    14401 aatggcaagg tttgtaacct gtaggcctga taagacgcgc aagcgtcgca tcaggcaaca
    14461 ccacgtatgg atagagatcg tgagtacatt agaacaaaca ataggcaata cgcctctggt
    14521 gaagttgcag cgaatggggc cggataacgg cagtgaagtg tggttaaaac tggaaggcaa
    14581 taacccggca ggttcggtga aagatcgtgc ggcactttcg atgatcgtcg aggcggaaaa
    14641 gcgcggggaa attaaaccgg gtgatgtctt aatcgaagcc accagtggta acaccggcat
    14701 tgcgctggca atgattgccg cgctgaaagg ctatcgcatg aaattgctga tgcccgacaa
    14761 catgagccag gaacgccgtg cggcgatgcg tgcttatggt gcggaactga ttcttgtcac
    14821 caaagagcag ggcatggaag gtgcgcgcga tctggcgctg gagatggcga atcgtggcga
    14881 aggaaagctg ctcgatcagt tcaataatcc cgataaccct tatgcgcatt acaccaccac
    14941 cgggccggaa atctggcagc aaaccggcgg gcgcatcact cattttgtct ccagcatggg
    15001 gacgaccggc actatcaccg gcgtttcacg ctttatgcgc gaacaatcca aaccggtgac
    15061 cattgttggc ctgcaaccgg aagaaggcag cagtattccg ggcattcgcc gctggcctgc
    15121 ggaatatctg ccggggattt tcaacgcttc gctggtggat gaggtgctgg atattcatca
    15181 gcgcgatgcg gaaaacacca tgctcgaact ggcggtgcgg gaaggaatat tctgtggcgt
    15241 cagctcaggc ggcgcggttg ctggagcact gcgggtggca aaagctaacc ctggtgcggt
    15301 ggtggtagcg atcatctgcg atcgtggcga tcgctacctc tccaccggag tgtttgggga
    15361 agagcatttt agtcaggggg cagggattta aggattaata gcattggaga ctgatgacaa
    15421 acgcaaaact gcctgatgcg ctccgcttat caggcctaca atgtttctgc aatatattga
    15481 attagcaata tttttatgcc ggatcaggcg tttacgccgc atccggcata aacaaagtgc
    15541 actttgtcaa taatctgatt tgtcggcagt tgttgctgcc gacaaatgca gtttttaatt
    15601 ttcgtgtgga aaagcagtaa ggtcatcaat cattttttga agtattggtg agtcctgacc
    15661 gtcaccccat tgaagaattt ttgcggtaag ctgatgacgc gctagtgcat tgagtcccat
    15721 ctttttaagt ttatgggaga ttttacccat gactttgatc tgaccgaatg cagtcgcgta
    15781 ggtaatcata ttgctctgga tagagctcat ttcatcggct agtaattgcg cacgcacatc
    15841 ctcttcaaga atactgtcca tagcgatgta tttcataccc cacatagttt caattaacat
    15901 ttgagggaaa tcagcacagt ccagaacgga ggctattttc aagttcgttt agagcatcag
    15961 caccttcatc gctaccaaaa ggagattctt catcccagca acacatataa agtggatcag
    16021 taaaatggca ctggaagttt gggtgtgacg ttttaagatg taacccaatc tcttcatcat
    16081 caaaatagta gagatccatg aagttaaagt ctggagtttc ttgatatcct tttttcattt
    16141 ttgcggcaat caatttactg gcttctttca gacactgttc ttcattatcg aattcttttg
    16201 tatggaattt accaatacta cctaccttgc catagttgac agctaaacta tccccttcgt
    16261 aatctatcca ccaaaattta ttcgattttt catcgtgaca aataaagcat tttttcatgt
    16321 tacctcctgt aaatgcctta ccaaagcgga ttaatttcct tccgcaggca ccgttttatc
    16381 catctgcgca attaacgcat gtaaaaaagg ctgtgcgttc tcatcgctct taccggggat
    16441 cttcacgata tacggcttcc cggtaatcga tgacgacgaa gctaccttat caataaactg
    16501 ctcggcagtg tcaatgcggt tacgggtatt gccgagcttc agacgcaggt gggaaaccgc
    16561 ttcatcgcag gtatgttcat caccgttgcg cacaaatatc aaatcctttt tctgtgctaa
    16621 tccctccagc atggcgttga tgcgggcttc ttcatgaaca gttaacttcg cgtaagcggg
    16681 gagtgtcatt agcagcgtaa tggccagaca aatgattttc ttcactgaat taccttatcc
    16741 ttgttttgta atagccgatt tgactgccag aatcaggtaa agtttcggtt ggaatacgat
    16801 gaggatcatc ggcaaggaga aaaaattaaa tgagtagttt gttgttgttt aacgataaga
    16861 gtagggcgct gcaggcggat atcgtcgccg tgcagtcgca ggtagtgtac ggaagcgtag
    16921 gcaacagcat tgcggtgcct gctataaaac agaacggctt gaatgtcttt gccgtgccga
    16981 cggtattgct gagcaatacg ccgcattatg acactttcta cggtggtgcg attccggacg
    17041 aatggtttag cggctatttg cgtgcgcttc aggagcgtga tgcgctgcgc caacttcgtg
    17101 ctgtaaccac gggctatatg ggaacggcat cgcaaatcaa aatccttgcc gagtggctga
    17161 ctgcgctacg caaagaccat cctgacctat tgatcatggt cgatccggtg attggcgata
    17221 ttgatagcgg aatttatgtc aaacctgacc ttcccgaggc gtatcgacaa tatttactgc
    17281 cgctggcgca gggaattacc cccaatatct ttgagctgga aatcctgacc ggtaaaaatt
    17341 gccgcgatct cgacagtgcc attgctgccg caaaaagtct gctttcagac acattaaaat
    17401 gggtggtgat taccagcgcc tccggtaatg aagaaaatca ggagatgcag gtggtggtgg
    17461 tcagtgccga cagcgtgaat gtcatttccc attcacgggt aaaaaccgac ctgaaaggga
    17521 ctggcgacct gttttgtgct cagcttatca gtggtttgct gaaagggaag gcgttaaacg
    17581 atgcggtgca ccgtgcgggg ttgcgcgtac tggaagtgat gcgctacacc cagcagcatg
    17641 agatcgatga attgattttg ccgccgctgg cggaagcata aaaaatggcg ccgatgg
//
LOCUS       DADLSA010000080        17467 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.080, whole genome shotgun sequence.
ACCESSION   DADLSA010000080 DADLSA010000000
VERSION     DADLSA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.080"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..670
                     /locus_tag="JN691_002600"
     CDS             <1..670
                     /locus_tag="JN691_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7244014.1"
                     /translation="LEIRAAGSKFAIANQYRALLSDIFKTAIASGLAEEDPASATRPF
                     RTEVKRSRLLIDEYLLIRKIADVQNEWFGLCMDLALVTGQREGDLAAMRWEDIRDGRL
                     YVEQQKTGAKIRISLPTTISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRI
                     ARDTSGLKWEGDPPPFHEIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGRE
                     WKDI"
     gene            906..1724
                     /locus_tag="JN691_002601"
     CDS             906..1724
                     /locus_tag="JN691_002601"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HAY7244015.1"
                     /translation="MTTRVIALDLDGTLLTLKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSRHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1725..2783)
                     /gene="modC"
                     /locus_tag="JN691_002602"
     CDS             complement(1725..2783)
                     /gene="modC"
                     /locus_tag="JN691_002602"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAY7244016.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2786..3475)
                     /gene="modB"
                     /locus_tag="JN691_002603"
     CDS             complement(2786..3475)
                     /gene="modB"
                     /locus_tag="JN691_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAY7244017.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3475..4248)
                     /gene="modA"
                     /locus_tag="JN691_002604"
     CDS             complement(3475..4248)
                     /gene="modA"
                     /locus_tag="JN691_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7244018.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4415..4564)
                     /locus_tag="JN691_002605"
     CDS             complement(4415..4564)
                     /locus_tag="JN691_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAY7244019.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4693..5481
                     /gene="modE"
                     /locus_tag="JN691_002606"
     CDS             4693..5481
                     /gene="modE"
                     /locus_tag="JN691_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAY7244020.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5549..7021
                     /gene="modF"
                     /locus_tag="JN691_002607"
     CDS             5549..7021
                     /gene="modF"
                     /locus_tag="JN691_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAY7244021.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7282..8298
                     /gene="galE"
                     /locus_tag="JN691_002608"
     CDS             7282..8298
                     /gene="galE"
                     /locus_tag="JN691_002608"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAY7244022.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8308..9354
                     /gene="galT"
                     /locus_tag="JN691_002609"
     CDS             8308..9354
                     /gene="galT"
                     /locus_tag="JN691_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAY7244023.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9358..10506
                     /gene="galK"
                     /locus_tag="JN691_002610"
     CDS             9358..10506
                     /gene="galK"
                     /locus_tag="JN691_002610"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAY7244024.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10500..11540
                     /gene="galM"
                     /locus_tag="JN691_002611"
     CDS             10500..11540
                     /gene="galM"
                     /locus_tag="JN691_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAY7244025.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLSPGEE
                     YSSLTEYQFIAE"
     gene            11743..12495
                     /gene="gpmA"
                     /locus_tag="JN691_002612"
     CDS             11743..12495
                     /gene="gpmA"
                     /locus_tag="JN691_002612"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAY7244026.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12662..13714)
                     /gene="aroG"
                     /locus_tag="JN691_002613"
     CDS             complement(12662..13714)
                     /gene="aroG"
                     /locus_tag="JN691_002613"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAY7244027.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            14030..14410
                     /locus_tag="JN691_002614"
     CDS             14030..14410
                     /locus_tag="JN691_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244028.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14524..15459
                     /gene="zitB"
                     /locus_tag="JN691_002615"
     CDS             14524..15459
                     /gene="zitB"
                     /locus_tag="JN691_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAY7244029.1"
                     /translation="MAHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGSLA
                     LLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVITIL
                     IVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHVLG
                     DLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPVSL
                     DIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYLMD
                     HYQIEHATIQMEYQPCHGPDCHLNEGGSGHSHHHH"
     gene            complement(15456..16175)
                     /locus_tag="JN691_002616"
     CDS             complement(15456..16175)
                     /locus_tag="JN691_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAY7244030.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16213..17256)
                     /gene="nadA"
                     /locus_tag="JN691_002617"
     CDS             complement(16213..17256)
                     /gene="nadA"
                     /locus_tag="JN691_002617"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAY7244031.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFVAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4447 a         4551 c         4351 g         4118 t
ORIGIN      
        1 tcttgaaatc agagcagctg ggagtaaatt tgctattgct aaccagtaca gggcgctttt
       61 atcagacatt tttaaaacag ctatagcttc tggtcttgcc gaagaagatc cagcttctgc
      121 aaccaggcca ttcagaactg aagtgaagcg cagtcgactg ttaattgacg agtatctgtt
      181 aatcaggaaa atagcagacg tccagaacga atggttcggc ctttgcatgg atcttgcact
      241 ggtaacagga cagcgcgaag gagatctggc ggctatgaga tgggaggata taagagatgg
      301 caggctatac gtggaacaac aaaaaacagg ggcaaaaatt cgcatttctc tccccaccac
      361 gatttccaga ttgaatttaa cacttgccga tgtactggat aacttgaaaa aaataaacgg
      421 taaaaacgaa aagttactcg gagggaagac agccaggaca atcgcagcac agttcaggat
      481 tgcccgagac acatccggat tgaaatggga aggtgaccca ccgccatttc atgaaatccg
      541 aagtttatcc ggtcgcctgc actcagcaga aaaaggcagt gatttcactc aggcgctcct
      601 tggacatcgt tcatccagca tgacggataa ataccgtgac ggacgaggga gggaatggaa
      661 agacatttag aattaatttc gaatgatttc gaaatgccac ttgtaattaa ttgattataa
      721 aggattaatt cctaaagagc atttgcttca tgaatgctcc tttgcattta gcaactgact
      781 gtaacagcta aaattagtcg cttttggcgg tgaatgctga attaaaacgc agaattttgt
      841 ccgatagtgg tatcggtgta ccattcgcgc agacgaaaat tcttaacctt aatctggaat
      901 acgccatgac cacacgcgtg attgctctcg acttagacgg caccttattg accctgaaaa
      961 agaccttgct tccatcatcg atagaagccc tggcccgcgc tcgcgaagca ggctatcaat
     1021 taatcatcgt cacaggtcgc catcacgtcg ctattcatcc tttttatcag gcgctggcgc
     1081 tggatacacc tgctatttgc tgtaatggca cctatttgta tgattatcat gcaaaaaccg
     1141 tgctggaagc ggacccaatg cccgttaata aagccctgca actcattgag atgctgaatg
     1201 aacaccacat tcacggtctg atgtatgtcg atgatgcaat ggtctatgag cacccgaccg
     1261 ggcatgtcat tcgcacgtct aactgggcgc aaaccctgcc gccggaacag cgtccgactt
     1321 tcacacaagt cgcttctctg gctgaaacgg cgcaacaagt taacgccgta tggaagttcg
     1381 ccctgacgca cgatgacctg ccgcaattgc agcattttgg taagcatgtc gaacatgaac
     1441 tgggactgga gtgtgaatgg tccaggcacg atcaggttga tattgcacac ggcggcaaca
     1501 gcaaaggtaa acgtttgacg aaatgggttg aggcgcaagg ttggtcgatg gaaaacgtcg
     1561 tggcattcgg cgataacttt aatgatatca gtatgctgga agccgctggt acaggcgtgg
     1621 cgatgggcaa cgccgatgac gcggtaaaag cgcgcgccaa cattgtgatt ggtgataaca
     1681 ccaccgacag cattgcccag ttcatttata gccacctgat ttaatcaggc ggttatcgac
     1741 acacttttaa tttgcgcgta cagccacagg ccaggtttga tcgccagttc atccctggcc
     1801 cacgggctga tacgcgccca cagcgtttta ccgccgactt ccagctccac ttccacttgg
     1861 ccgttgtcgt cataactatt aacaactttt gcccgcaata cgttacgaat gctggtttgc
     1921 tgcggcggtt gtaaaaccag agaaacatcg gaagcctgaa tgcggatacg tagcgcagct
     1981 tgcagcggtt cgcccagctt attgacccac aaatgctgat cgcccagcgc cagcgcggtc
     2041 atcgcgtaat gcggatgatg ctcaagcacc gtcactttca gaatgctact ttgttgctct
     2101 ttcggcagcc acggattcat cacgctactg ccccacactt cctccagcgc gccaaaggct
     2161 ttcacctgac cgttttccag taccatcact ctgtctgcca gatggaggat ctcatccagc
     2221 gaatggctga catacaacat cggaatgttg atttcccgtg tcagccgttg cagataaggc
     2281 aacagttcgc gtttacgcgg aatatccagt gacgccagcg gttcatccag caacagcaat
     2341 tccggtgctg tcagcaaagc ccgaccaatc gccacgcgct gtttttcgcc tccggacagg
     2401 ctgcctggta aacggtcaag caacggttca atgcctaaaa gcgccaccag cttatcgaac
     2461 tgatcgacca tacttttcga catgccgtag cgcagattgc cacgcacttt gtaatgcggg
     2521 aacagccgcg catcctgaaa aacatagcca acgcgacgct tttccggcgt caggcagata
     2581 cctttttcgg catcatttag tacccgccca ttgaggacaa tccgcccttt ttgcgggcgc
     2641 gtcagtccac tgatggcgtt aatcagcgaa gtttttccgg caccggagac gccaaagata
     2701 gcagtgatgc cattggcggg cagcgtttca ttaatggtca ggcaatggtt gcccaacgtc
     2761 tgggaaaaat tcagctccag catgattagc gccccgcccg ttcacggctg attctggcca
     2821 gccattctga aatcaacagg gagatcatcg ccagcgcaat agaaataatg cacagtctcg
     2881 ccgcgccact ttccccgccg ggggtctgga tcagggtata catggcagaa ggaatggttc
     2941 gcgtttcgct aggaatgttc gacacaaagg tgatggttgc accaaactca ccgagagaac
     3001 gagcaaaagc cagtaccgta ccaacaataa ttcccggtaa ggtcagcggt aacgtgatag
     3061 taaagaaaac gcgccagcgc ccggcaccta gtgttcttgc ggcctgttcc agtttgacat
     3121 caaccccttc cagcgccaga cgaattgccc gcaccatcag cggaaacgac atgacggcgg
     3181 cagcgagaac cgcgccgcgc cagctaaagg cgaaggtaat accaaaccag tcatacagac
     3241 gttcaccgat aaatccgcgc cgtcccatcg aaactaataa taagtaaccg acgaccacgg
     3301 gcggtaacac cagcggtaga tgcagtacgc tgtcgagcag agctttgccc ggaaacgtgc
     3361 aacgcaccag taaccaggca aaaaagatcc caaacggcag gctaaacagc acagccaggg
     3421 aagaaacttt caggcttaat aaaactgcct gccattctgg atcggtcagt atcattactt
     3481 ggttgtaaat ccgtaacgtt taaagatttc cgctgcctgc ggtcccttca gataatcata
     3541 aaatgctttc actgtcgcat tgttatgccc ttccacaaca gcaaccggat attccacttt
     3601 tttatgtgaa tcttccggga aggtggcaac cacttttacc cctttgctgg caactgcgtc
     3661 agaaccgtag acaatgccca gaggcgcttc gttacgttcg accagcgcca gcgccccacg
     3721 aacatcttcc gctggggcca gtttcggaga gagcgtatcc catgcgccca gtttttgcag
     3781 tgcttctttt gcataaatgc cagcgggaac atgttccgga tcgccaaccg ccaggcgacc
     3841 gccattcagc agcgaagtcc agttagtttt gctgtcgatg gtgaaatctt tctgctcgct
     3901 ggctttcggt gctacaacga ccaggctatt gccgagcagt gtctgacgcg tagcagtatc
     3961 gatcgctttt ttatcaaccg cataatccat ccatttctga tcggcagaaa taaacagatc
     4021 cgcaggggca cccgcttcaa tctgacgggc gagagtagat gacgaagcga aagaagaaac
     4081 cacatccacg cctttctctt ttttatactg cgtagcaatg tcctgcattg cgttagtcag
     4141 tgatgcggcg gcgaacaccg tgattttccc ttcatctgcc agtgcattgc cagcaactgc
     4201 gaaagagagt gccgccccgg caaacaagtt caaccattta cgagccattg gtaacccctt
     4261 aatgtaacgt tgtgtaggcg acaatataac gactaactcg gaattttccc agcagttatt
     4321 gctaaccttt gtgaggtaga taagaaaaaa tatcggcagg aaaagcagga agttgagaaa
     4381 aagaaaatgc ccgactaagc gggcattcag ggaatcaatg attttgtccg ggctggtctt
     4441 ttttaccaac accagaaaag atgttgaata cttcaccaag accgtaaatc agacccagga
     4501 tgatggccat cacgacaggt accatgatta cggcgaatac cagacttttt aataactcta
     4561 acatggtcaa ctccagatat agtcatgaga ctattctaac cgctaagcac agaaaagcac
     4621 tccccttttg tgcggtcagc tttgcgtggc gttcgttttc cgtcacaata agacttttgc
     4681 caggacattg ttatgcaggc cgaaatcctt ctcaccctta agctccaaca aaaattattc
     4741 gccgacccgc gccgcatttc gctactaaaa cacattgcgc tttccggttc cattagccag
     4801 ggagcgaaag atgccggtat cagctataaa agcgcctggg atgccattaa cgagatgaat
     4861 cagttaagtg agcatattct ggtcgagcgc gcaacaggcg gtaaaggtgg cggaggcgca
     4921 gtactgaccc gctatggtca gcgactgatt cagctctatg acttactggc gcaaatccag
     4981 caaaaagcct ttgatgtgtt aagtgacgat gacgccctgc cgctgaacag cctgctggcc
     5041 gcgatctcac gtttttcact gcaaaccagc gcccgtaacc agtggttcgg taccatcacc
     5101 gcccgcgatc atgatgacgt tcaacagcat gttgatgtct tactggctga cggaaaaaca
     5161 cgcctgaaag tcgcaattac cgcacaaagc ggcgcacgtc tggggctgga tgaaggcaaa
     5221 gaagtgttga tattgctaaa agcgccgtgg gtaggcatta ctcaggacga agcggtcgca
     5281 caaaacgccg acaaccaatt accgggtatt attagtcata ttgagcgcgg cgcagagcag
     5341 tgcgaagtat taatggcgct acccgacggg caaacactgt gcgccacagt gccggtaaat
     5401 gaagcgactt ctcttcagca agggcagaat gtcacggcct actttaatgc cgacagcgtg
     5461 attatcgcca cgctgtgcta agcgtgttga caatttgtta tgaaacacgt atccctgtca
     5521 gtaatcgctg cacaaaatgg gatataaaat gtcatcgttg caaattttgc aaggcacgtt
     5581 tcgtcttagc gacacaaaaa cgctgcaatt gcctcagcta acgttaaacg cgggtgatag
     5641 ttgggcgttt gtcggttcga atggaagtgg aaagtcggcg ctggcccgcg cgctggcggg
     5701 ggaacttccg cttttgaaag gtgaacggca aagccagttt tcccacatca ctcgtctctc
     5761 cttcgagcaa ttgcaaaagc tcgtcagcga cgaatggcaa cggaataaca ccgatatgct
     5821 cggccctagc gaagatgaca ccggacgcac tacggctgag attattcagg atgaagtaaa
     5881 ggatgcaccg cgttgcatgc aactggcgca gcagttcggt attaccgccc tcctcgaccg
     5941 acgctttaaa tacctttcca ctggcgagac gcgaaaaacc ctgctgtgtc aggcgctgat
     6001 gtcggagcct gacttgttga ttcttgatga gccgttcgat ggcctggatg ttgcctcacg
     6061 tcagcagctg gctgagcgac tcgcctcgtt acatcagtcc ggtattactt tggtactggt
     6121 gctcaatcgc ttcgatgaga tcccggaatt tgtccagttt gctggcgtgc tggcggattg
     6181 tacgttagcg gaaactggcg ctaaagagga actgctccag caagcactcg tcgcgcaact
     6241 tgcgcatagt gaacagcttg aaggtgtgca actgccggag ccggatgaac cttcagcacg
     6301 tcacgcctta cccgccaacg aatcgcgcat tgtgctgaac aatggcgtgg tttcttataa
     6361 tgatcgcccc attcttaata accttagctg gcaggtgaat ccaggcgaac actggcaaat
     6421 tgtcgggcca aatggcgcgg gaaaatcgac gttattaagc ctgattactg gcgatcatcc
     6481 gcaaggttac agcaacgatt tgacgctttt cggccgacgt cgcggcagca gcgaaaccat
     6541 ctgggacatc aaaaagcata tcggttacgt cagcagtagt ttgcatctgg attaccgggt
     6601 cagcactacc gtgcgtaatg tgattctttc tggctatttt gattcgattg gcatttatca
     6661 ggccgtttcg gatcgccagc aaaaactggt gcagcagtgg ctggatattc tcggcattga
     6721 taaacacacg gctgacgctc cgttccatag tctttcctgg ggacagcagc gtctggcgct
     6781 gatcgtccgc gcactggtga aacatccgac gttgcttatt ctcgatgaac cactacaggg
     6841 gcttgatccg ctcaatcgcc aacttatccg ccgttttgtt gatgtgctga ttagcgaagg
     6901 tgaaacgcaa ttgttgtttg tttcgcacca cgctgaagat gcgcctgcct gtattaccca
     6961 tcgccttgag ttcgtgccgg acggtggact ctatcgctat gtgctgacaa aaatatattg
     7021 agtcggtagt gctgaccttg ccggaggcgg cctaagcgcc ctctccggcc aacggttcga
     7081 cgcatgcagg catgaaaccg cttctttttt cagataaaaa gcgcaatcat tcataaaccc
     7141 tctgttttat aatcacttaa tcgcgcataa aaaacggcta aattcttgtg taaacgattc
     7201 cactaattta ttccatgtca cacttttcgc atctttgtta tgctatggtt atttcatacc
     7261 ataagcctaa tggagcgaat tatgagagtt ctggttaccg gtggtagcgg ttacattgga
     7321 agtcatacct gcgtgcaatt actgcaaaac ggtcatgatg tcatcattct tgataacctc
     7381 tgtaacagta agcgcagcgt actgcctgtt atcgagcgtt taggcggcaa acatccaacg
     7441 tttgttgaag gcgatattcg taacgaagcg ttgatgaccg agatcctgca cgatcacgct
     7501 atcgacaccg tgatccactt cgccgggctg aaagccgtcg ccgaatcggt acaaaaaccg
     7561 ctggaatatt acgacaacaa tgtcaacggc actctgcgcc tgattagcgc catgcgcgcc
     7621 gctaacgtca aaaactttat ttttagctcc tccgccaccg tttatggcga tcagccccaa
     7681 attccatacg ttgaaagctt cccgaccggc acaccgcaaa gcccttatgg caaaagcaaa
     7741 ttgatggtgg aacagatcct caccgacctg caaaaagccc agccggactg gagcattgcc
     7801 ctgctgcgct acttcaaccc ggttggcgcg catccgtcgg gcgatatggg cgaagatccg
     7861 caaggcattc cgaataacct gatgccatac atcgcccagg ttgctgtagg ccgtcgcgac
     7921 tcgctggcaa tttttggtaa cgattatccg accgaagatg gtactggcgt acgcgattac
     7981 atccacgtaa tggatctggc ggacggtcac gtcgtggcga tggaaaaact ggcgaacaag
     8041 ccaggcgtac acatctacaa cctcggtgct ggcgtaggca gcagcgtgct ggacgtggtt
     8101 aatgccttca gcaaagcctg cggcaaaccg gttaactatc attttgcacc gcgtcgcgaa
     8161 ggcgaccttc cggcctactg ggcggacgcc agcaaagccg accgtgaact gaactggcgc
     8221 gtaacgcgca cactcgatga aatggcgcag gacacctggc actggcagtc acgccatcca
     8281 cagggatatc ccgattaagg aacgaccatg acgcaattta atcccgttga tcatccacat
     8341 cgccgctaca acccgctcac cgggcaatgg attctggttt caccgcaccg cgctaagcgc
     8401 ccctggcagg gggcgcagga aacgccagcc aaacaggtgt tacctgcgca cgatccagat
     8461 tgcttcctct gcgcaggtaa tgtgcgggtg acaggcgata aaaaccccga ttacaccggg
     8521 acttacgttt tcactaatga ctttgcggcc ttgatgtctg acacgccaga tgcgccagaa
     8581 agcaacgatc ctttgatgcg ttgccagagc gcgcgcggta ccagccgggt gatctgcttt
     8641 tcaccggatc acagtaaaac gttgccagaa ctgagcgttg cggcattgac ggaaatcgtc
     8701 aaaacctggc aggagcaaac cgcagagctg ggaaaaacgt acccgtgggt gcaggtcttt
     8761 gaaaacaaag gtgcggcgat gggctgctct aacccgcatc cgcacggaca gatttgggca
     8821 aatagcttcc tgcctaacga agctgagcgc gaagaccgcc tgcaaaaaga atattttgcc
     8881 gaacagaaat caccaatgct ggtggattat gttcagcgcg agctggcaga cggtagccgt
     8941 accgttgtcg aaaccgaaca ctggttagcc gttgtgcctt actgggctgc ctggccgttc
     9001 gaaacgctac tgctgcccaa agcccacgtt ttgcggatca ccgatttgac cgacgcccag
     9061 cgcagcgatt tggcgctggc gttgaaaaag ctgaccagtc gttatgacaa cctcttccag
     9121 tgctccttcc cctactctat gggctggcac ggcgcaccat ttaatggcga agagaatcaa
     9181 cactggcagc tgcacgcgca cttttatccg cctctgctgc gctccgccac cgtacgtaaa
     9241 tttatggttg gttatgaaat gctggcagag acccagcgag acctgaccgc agaacaggca
     9301 gcagagcgtt tgcgcgcagt cagcgatatc cattttcgcg aatccggagt gtaagaaatg
     9361 agtctgaaag aaaaaacaca atctctgttt gccaacgcat ttggctaccc tgccactcac
     9421 accattcagg cgcctggccg cgtgaatttg attggtgaac acaccgacta caacgacggt
     9481 ttcgttctgc cctgcgcgat tgattatcaa accgtgatca gctgtgcacc acgcgatgac
     9541 cgtaaagttc gcgtgatggc agccgattat gaaaatcagc tcgacgagtt ttccctcaat
     9601 gcgcccattg tcgcgcatga aaactatcaa tgggcgaact acgttcgtgg cgtggtgaaa
     9661 catctgcaac tgcgtaacaa cagcttcggc ggtgtggaca tggtgatcag cggcaatgtg
     9721 ccgcagggtg ccgggttaag ttcttccgct tcactggaag tcgcggtcgg aaccgtattg
     9781 cagcagcttt atcatctgcc gctggacggc gcacaaatcg cgcttaacgg tcaggaagca
     9841 gaaaaccagt ttgttggctg taactgcggg atcatggatc agctaatttc cgcactcggc
     9901 aagaaagatc atgccttgct gattgactgc cgctcactgg ggactaaagc agtttccatg
     9961 ccgaaaggtg tggctgtcgt catcatcaac agtaacttca aacgtaccct ggttggcagc
    10021 gaatacaaca cccgtcgtga acagtgcgaa accggtgcgc gtttcttcca gcagccagct
    10081 ctgcgcgatg tcaccattga agagttcaac gctgttgcac atgagctgga cccaatcgtg
    10141 gcgaaacgcg tgcgtcatat cctgactgaa aacgcccgca ccgttgaagc tgccagcgcg
    10201 ctggagcaag gcgacctgaa acgtatgggc gagttgatgg cggagtctca tgcctctatg
    10261 cgcgatgatt tcgaaatcac cgtgccgcaa attgacactc tggtagaaat cgtcaaagct
    10321 gtgattggcg acaaaggtgg cgtacgcatg accggcggcg gatttggcgg ctgtatcgtc
    10381 gcgctgatcc cggaagagct ggtgcctgcc gtacagcaag ctgtcgctga acaatatgaa
    10441 gcaaaaacag gtattaaaga gactttttac gtttgtaaac catcacaagg agcaggacag
    10501 tgctgaacga aactcccgca ctggcacccg atggtcagcc gtaccgactg ttaactttgc
    10561 gtaacaacgc agggatggta gtcacgctga tggactgggg tgcgacttta ctttccgccc
    10621 gtattccgct ttccgatggc agcgtccgcg aggcgctgct cggctgtgcc agtccggaat
    10681 gctatcagga tcaggctgcg tttctggggg cctctattgg tcgttatgcc aaccgcatcg
    10741 ccaatagtcg ttataccttt gacggtgaaa ccgtgacgct ttcgccaagc cagggcgtta
    10801 accagctgca cggcggcccg gaagggttcg acaaacgtcg ctggcagatt gtgaaccaga
    10861 acgatcgtca ggtgctgttt gccctgagtt cagatgatgg cgatcagggc ttcccgggta
    10921 atctcggcgc gacggtgcaa tatcgtctga ccgacgataa ccgtatctcc attacttatc
    10981 gcgccacagt tgataaacct tgcccggtga atatgactaa tcacgtctat ttcaatcttg
    11041 acggcgagca gtctgacgtg cgcaatcaca agttgcagat tctggcggac gaatatcttc
    11101 cggttgatga aggcggcatt ccgcacgacg gcttgaaatc tgtcgccgga acgtcttttg
    11161 atttccgcag cgccaaaatc atcgccagtg agtttcttgc cgacgacgat cagcgcaaag
    11221 tgaaaggtta cgatcacgca ttcttgttac aggccaaagg cgatggcaag aaagtggcgg
    11281 cgcatgtctg gtcagcagat gaaaaattgc agctgaaggt ctacaccacc gctccggctc
    11341 ttcaattcta ctccggcaac ttcctcggcg gcacaccgtc gcggggaacc gaaccttacg
    11401 ccgactggca agggctggcg ttggaaagcg agtttctgcc ggacagcccg aaccaccctg
    11461 aatggccgca accggactgc ttcctgagtc ctggcgaaga gtattccagc ctgacggaat
    11521 atcagtttat tgctgagtaa tgttaagccc tcgtcatgag ggctttatct tcatattgtt
    11581 caaatcacca gcaaacaccg acatatttgc aactcaatat tcacaacaac cttacactgc
    11641 gccactattt tcgctatggt tatgcgtaag cattgctgtt gcttcgtcac ggcaatataa
    11701 tgagaattat tatcattaaa agatgatttg aggagtaagt atatggctgt aactaagctg
    11761 gttctggttc gtcatggcga aagtcagtgg aacaaagaaa accgtttcac cggttggtac
    11821 gacgtggatc tgtctgagaa aggcgtaagc gaagcaaaag cagcaggtaa gctgctgaaa
    11881 gaggaaggtt acagctttga ctttgcttac acttctgtgc tgaaacgcgc tatccatacc
    11941 ctgtggaatg tgctggacga actggatcag gcatggctgc ccgttgagaa atcctggaaa
    12001 ctgaacgaac gtcactacgg tgcgttgcag ggtctgaaca aagcggaaac tgctgaaaag
    12061 tatggcgacg agcaggtgaa acagtggcgt cgtggttttg cagtgactcc gccggaactg
    12121 actaaagatg atgagcgtta tccgggtcac gatccgcgtt acgcgaaact gagcgagaaa
    12181 gaattgccgc tgacggaaag cctggcgctg accattgacc gcgtgatccc ttactggaat
    12241 gaaaccattc tgccgcgtat gaagagcggt gagcgcgtaa tcatcgccgc acacggtaac
    12301 tccctgcgtg cgctggtgaa atatcttgat aacatgagcg aagaagagat tcttgagctg
    12361 aatatcccga ctggcgtgcc gctggtgtat gagttcgacg agaatttcaa accgctgaaa
    12421 cgctattatc tgggtaatgc tgacgagatc gcggcgaaag cagcagccgt tgcgaaccag
    12481 ggtaaagcga agtaaacgtc attcgtttaa aaaatgagaa agccgactgc aagtgagtcg
    12541 gcttttttgt ttgtcgttgc taacaatgga gcacattgcc tgatgcgacg ctgcgcgtct
    12601 tatcaggcct gtggtgattc atcggatacg ccactctgac ggcgcatccg acaattaaac
    12661 cttacccgcg acgcgctttt actgcattcg ccagttgacg taacagagca tcggtatctt
    12721 cccagccaat gcaggcatcg gtgatgctct taccgtaggc cagcggctcc ccgctatcga
    12781 ggctctgatt gccttccacc agatggcttt ccaccatcac gccaataatg gccttttcgc
    12841 caccggcaat ctgctggcaa acgtcagcac aaacatccat ctgctttttg aattgtttgg
    12901 acgaattagc atggctgaaa tcgatcatca cctgagctgg cagacctgct ttgttcagcc
    12961 cttctttcac ttcagcaacg tgcttcgcgc tgtagttagg ctctttaccg ccgcgcagaa
    13021 tgatatggca atcgccgtta ccactggtat tcacaatcgc cgaatgcccc catttagtta
    13081 cggacaggaa gcagtgcggc gcaccggcgg cattaatggc atcgatagcc actttaatcg
    13141 taccgtctgt gccattttta aaacctaccg gacaagaaag accagacgcc agttcgcggt
    13201 gcacctgcga ttccgtggta cgtgcaccaa ttgcgcccca gctcatcagg tcagcgagat
    13261 actgaggagt gatcatatcg agaaactcac ccgccgctgg cagaccgctg tcgttaatat
    13321 caagcagcaa tttacgggct atacgcagac cgtcgttgat ctggaagctg ttatccatat
    13381 gcggatcgtt aatcaaccct ttccagccca ccgtggtacg cggcttttca aaatagacgc
    13441 gcattacgat ttccagctca tctttcagct cttcacgcag cgccagcaag cgagtggcat
    13501 actctttagc cgcgacagga tcatgaattg agcacgggcc aatcaccacc aacaggcgat
    13561 catcattacc tttcaggatc ttatggatcg cttttcgggc atgggcaacc gtattcgcgg
    13621 cattttcagt tgcggggaat ttttccagca atgcgacagg aggaagtaac tctttgattt
    13681 ctttgatgcg taaatcgtcg ttctgataat tcatgtctgt tccagtgttg ccatacttat
    13741 cttagtgaat gcaatacttc caatctatat cttccgtcag aatgtgtaaa cggggtttta
    13801 cactatgaac ggattaatcc tggaattagc aagaaaaacg ccagattgtc gcgaaaaacg
    13861 agatctatcc tacaatttct aactgtaact cctttgtttg ttaattattt caagattctc
    13921 tgctgcgttt cataaactgg ctgaaatctt aaaccaatgc cttatattca cctgcaaatg
    13981 cactgttgga agaggttatc cgacataacg accataacag gagcatccta tgaaaatgac
    14041 aaaactggcc acattatttc tgactgccac tctaagcctt gccagcggtg ccgcactggc
    14101 tgccgatagc ggagcgcaaa ctaataacgg ccaggcaaac gccgcagctg atgcgggcca
    14161 ggtagcccct gacgcccgtg aaaatgtcgc gccaaataac gtcgacaata acggggtaaa
    14221 taccggttct ggcggcacaa tgctgcattc ggatggttct tcaatgaaca atgacggaat
    14281 gaccaaagat gaagagcaca agaacaccat gtgcaaagat ggtcgatgtc cggatatcaa
    14341 taaaaaggta caaaccggtg atggcatcaa taatgatgtc gataccaaaa ccgacggtac
    14401 cacgcagtaa ccctgcaaaa tagccaataa tgggagagcg gagtctctcc cttttaattt
    14461 ttcacttcta aaagcagtat gatgtcataa agttaagtga gaataattaa gggatgactc
    14521 gtcatggcgc actcacacac gtcctcacac ctgccagaag ataataatgc tcgtcgcttg
    14581 ttgtatgctt tcggcgtgac tgccgggttt atgctggtag aagtcgttgg tggttttctt
    14641 tctggttctc tggcattgct ggccgatgcg ggtcatatgt tgaccgatac tgccgccctg
    14701 cttttcgccc tactcgccgt gcaattttca cgtcgtcctc ccaccattcg ccacactttc
    14761 ggttggctaa gactgaccac gctcgcggcg tttgtgaatg ccatcgccct cgtggtaatt
    14821 accattttga ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc ggtcgaggga
    14881 ggcatgatga tggcaattgc cgtggctggg ttgctggcaa atatactttc tttctggtta
    14941 cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag cagcggcact gcatgtgctc
    15001 ggggacctgc tgggttcggt tggggctatt attgccgcgt tgattattat ctggactggc
    15061 tggacacctg ctgaccccat tctctcgata ctggtgtcgc ttctggttct gcgtagcgcg
    15121 tggcgactgt tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt atcgctggat
    15181 atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa tgtgcaccat
    15241 gtacatgtat ggatggtagg cgagaagccg gtgatgacgt tgcatgtgca ggtgatcccg
    15301 ccacacgatc acgatgcctt gttggatcag atccaacact acctgatgga tcactatcag
    15361 attgagcacg ccaccattca gatggaatat caaccttgtc atgggccaga ctgccacctg
    15421 aatgaagggg ggtctggcca ttcacatcat caccattaat gggacagcgc gcgtgagcct
    15481 ctttcacgtg cgctgttgat ccacatccgg ctgccgttga gcgcaataaa ggtcaggatg
    15541 atgtactcca gagacatggc gtaaacgccc tgaagtgcaa aaataacgac gctaatcacg
    15601 ttaataatca cccacaacag ccagttttcc acatacttac gcgtcatcag aatcattgcc
    15661 acgatagata acaccatcat gcatgaatcc cagaacggga aagcgtccgg ttgcagttca
    15721 ggcatcacaa cctgtaatcc taatgcttgc atgatcatga ctgccacgcg ggtcaaaaat
    15781 gcaaacaccg gattgataaa taccgtcatc agaccaatcg aaacaacgca aaccgccaac
    15841 cagctgagtg ccttcggcaa tggcaaccag cgaattttca actccgcctc gttctgactg
    15901 gtttgtcgcg accacgcata ccagccgtaa atattcgcgg caaagaaaaa cacctgtaat
    15961 agcaggctgg catacagctg aatctgaaag aaaataatgc caaacaaggt aacgttgatc
    16021 aggccaaaga agtagttgct gatcttctcc agactggcaa ggccaataca cagcaacccg
    16081 gcgatcgtgc ctaccgcttc aatccatgag agatcataac cgcccgcccc tattggtata
    16141 tgtaccagga tattctgcac actaaaaaaa tccatctttc ccccaaagcg taacgttacg
    16201 ccttattatt cgttatccac gtagtgtagc cgcaaaatcc agcatacgat tgagcggcac
    16261 cagcgccctc tctcgcagcc tctcatcgac atgaacctcg tgattacttc cttcctgttc
    16321 taatgcctct gcgatagcct gaaggccatt catggccatc cacggacaat gcgcgcagct
    16381 gcggcaggtt gcgccctcac ccgcggttgg tgcttccagt aactctttat ctggcaccgc
    16441 ctgctgcatt ttgtagaaaa taccccgatc ggttgccaca ataagcctct gatgaggcaa
    16501 tgcttttgca gcagcgatca gttgactggt ggaaccgacc gcatccgcca tatcgacaat
    16561 agcttgtggt gattctggat gcaccagtat ggcagcatcc gggtattctt cttgcaagcg
    16621 ggttaacgcc tgagtcttaa attcatcatg cacaatgcag gcaccctgcc agcataaaat
    16681 gtctccaccc gtctgttttt gcacgtaacg ccccagatgt ttgtcgggtg cccagatgat
    16741 tttttcaccc aaactatcaa gatgatcaat aagttcgacg gcaatgcttg aagttaccac
    16801 ccaatctgcg cgcgctttta ccgcagcaga agtgttggcg tagacgacga cagtacgatc
    16861 gggatgggca tcgcaaaatg cgttaaattc ttcaacaggg cagccgagat ccagtgaaca
    16921 ctcagcctga agtgtcggca tcagaattgt tttttccgga ctgagaattt tggcggtttc
    16981 tcccataaat ctcaccccag cgactaacaa agtagaagcg ggatgctttg caacgaagcg
    17041 cgccatttcc agagaatcag aaatacagcc accagtttct tctgccagtt gttgaatttc
    17101 gggatcggta tagtagtggg caaccatcac cgcattacgt tcttttagca gacgttttat
    17161 cttctcgcgg taatacgctt tttcatcaat gcttaacggc gtcggcttcg gggggaaagg
    17221 ataaatcgcc gtgtctggat caaacattac gctcatctta ccatctcgtt ttacaggctt
    17281 aacgttaaaa ccgacattag cgtaacattc gctgttatcg gtgtaatttg ttttatatgc
    17341 taaacaagat agtcgaatgg tggggaaaag tcactcgaat tacgcctaat tccaccgacg
    17401 tagagttgct tgcacagtaa gctctgacga tgtcatcagg tttacaaagt gaaattgagg
    17461 atgcctg
//
LOCUS       DADLSA010000081        17305 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.081, whole genome shotgun sequence.
ACCESSION   DADLSA010000081 DADLSA010000000
VERSION     DADLSA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.081"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(31..2478)
                     /gene="glgP"
                     /locus_tag="JN691_002618"
     CDS             complement(31..2478)
                     /gene="glgP"
                     /locus_tag="JN691_002618"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAY7244032.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQVIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="JN691_002619"
     CDS             complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="JN691_002619"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAY7244033.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRALRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="JN691_002620"
     CDS             complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="JN691_002620"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAY7244034.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEASLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="JN691_002621"
     CDS             complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="JN691_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAY7244035.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="JN691_002622"
     CDS             complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="JN691_002622"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAY7244036.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9672..10775)
                     /gene="asd"
                     /locus_tag="JN691_002623"
     CDS             complement(9672..10775)
                     /gene="asd"
                     /locus_tag="JN691_002623"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY7244037.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10967..11560
                     /gene="yhgN"
                     /locus_tag="JN691_002624"
     CDS             10967..11560
                     /gene="yhgN"
                     /locus_tag="JN691_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAY7244038.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="JN691_002625"
     CDS             complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="JN691_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY7244039.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVL
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="JN691_002626"
     CDS             complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="JN691_002626"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAY7244040.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="JN691_002627"
     CDS             complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="JN691_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HAY7244041.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14846..15541)
                     /locus_tag="JN691_002628"
     CDS             complement(14846..15541)
                     /locus_tag="JN691_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY7244042.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15664..16701)
                     /locus_tag="JN691_002629"
     CDS             complement(15664..16701)
                     /locus_tag="JN691_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7244043.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17034..>17305
                     /locus_tag="JN691_002630"
     CDS             17034..>17305
                     /locus_tag="JN691_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="YhhY; regulated by the fur regulator; unknown
                     function; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7244044.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRW"
BASE COUNT         4248 a         4739 c         4310 g         4008 t
ORIGIN      
        1 cctttggccc cgttctattt attagtgaaa ttacaatctc accggatcga tatgccagat
       61 atgatcagcg tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt
      121 cagcatcgct tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg
      181 acaatcgaca tagctgcgat aatccgccag tacctggtag tgatcgccga agttgatcag
      241 cgaatcaacc agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat
      301 ttgcgtcagc acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc
      361 ctgacgacgc agttcttcca cttcttccgc tgtgttacca aaaataaaga tattgtcagc
      421 accgacatga tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc
      481 gttaagcgca aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg
      541 ttcagacaga tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat
      601 gaacacgact ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc
      661 attgatcaaa tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa
      721 aatattcacg cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt
      781 aatcacatgc aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac
      841 atcgaacaac gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag
      901 acgctttttg ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca
      961 gtgttgttgc agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc
     1021 gtccagcacg gctgaaagcg atgggttcgc taccgccagc cagcgacgcg gcgtcacacc
     1081 gttggtgacg ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg
     1141 caccatcaga ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc
     1201 cagccaggcc atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag
     1261 cagatcggta tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc
     1321 aaagatgacc tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc
     1381 cagcgcctcg ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa
     1441 cgcgtcgtcc cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga
     1501 cagtaccgga tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt
     1561 tttatgcaac tgataatggc ggcttaaaat gtcctgaatg gtcgaggaaa ccaggaagta
     1621 ttcctgacgc aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg
     1681 agatacgttc tcggagtggt ttttatcttc cactgccgcg aagtagtcac cctggttgaa
     1741 tttaccgagg ttaatttcgc tactggcttg cgcactccac aaacgcagcg tgttggtcgc
     1801 gtcggtgtcg taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc
     1861 aatccagcgc gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg
     1921 cgtgttgtgg cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc
     1981 tttctggcta ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta
     2041 accgcgcccc ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc
     2101 caggccaccg ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa
     2161 ccccatcgct tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt
     2221 ggagagcgta cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg
     2281 cgacaactgg gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa
     2341 taacgttgcg ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa
     2401 catcagcttg taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata
     2461 tgtaaacgga gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc
     2521 acggtacgac ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac
     2581 aaaccgccac agtgaaggac gggaccacag tacaaaagca cgccgaagag cccgtaacag
     2641 cgaccaggca ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc
     2701 aagagaacag tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt
     2761 accgtacttc aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag
     2821 aatgacgtcc gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac
     2881 gcccacctga ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc
     2941 gcccgcgccg agtagcgcca gctgcccgcc ctgctccaga agccccggta aggcttccag
     3001 caccaggtcg agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt
     3061 atcgtcaacc ttaagcccca ttgcgatttg taactggcgc ttattttccg ctttatcttc
     3121 caacgtatcg cgggtgtaac gcgaggccaa cagtaagtcc gtctctggac tccagatttt
     3181 ctcgtccacg ccgttcagta cgcctgaaag acgcccttca cggtgacgct gttgcaacag
     3241 accttccata ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac
     3301 cgccgtaatg tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa
     3361 ttccagccca tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc
     3421 ataaaacatg ccttgatagg ccaggttgtg cactgtaaac accgacttcg caggacgccc
     3481 gcgcgccgcc agatacgcag gcgcaagacc tgcatgccag tcgtgcgcat gcaccacatc
     3541 aggacgccag aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagcagcgc
     3601 aaaacgcaat acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg
     3661 acgatcatag agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa
     3721 caacagcgtg atatgcccgg cgaaagtatc acgacgggat actacctgcg catcggtcac
     3781 gccacggcga atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat
     3841 ttgtgctgcg ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa
     3901 catctctgaa catacatgta aaacctgcat tatcgctcct gtttatgccc taacttccgt
     3961 agcatttcgc gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct
     4021 tcctctgcgt tttcaccaat caccatgcct tccgggataa cacaagcacg atcgatgacg
     4081 cagcggcgca gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca
     4141 atgttgcaga atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa
     4201 ccggagatca cacaaccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag
     4261 cgatcctgca cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg
     4321 cgatcgtaca tatccagttc cggcaccaca gaggccagat cgagattcgc tttccagtaa
     4381 gcttccagcg tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa
     4441 gagagcggga acgggtgcgc ataggccaga ctggcttcgg tgatcttggg aatcaaatct
     4501 ttgccaaagt cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga
     4561 tagtcggcgt caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc
     4621 attgacggtg ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc
     4681 gccataacgc caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa
     4741 cgtgcgcctt tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg
     4801 tcgcccgcca ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg
     4861 gtgaccgcat ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc
     4921 agcagatcga caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc
     4981 tgcaccagag tgtgggactg gtactgggtg atcacgccca tacgacggat cccagagttg
     5041 atgcagttag acagcgcaaa gtcaataatg cggaacttac cgccgaagtg tacggccggt
     5101 tttgctcgct tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg
     5161 gcaacagatt tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta
     5221 accatgacta actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct
     5281 gccagacaac cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact
     5341 ccccagcagg taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc
     5401 gatccgagag cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag
     5461 gttgagcata tcgatttagc caacggacat tgccgtcacc ttcttcccac cagcgattct
     5521 ccaccaaagc gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac
     5581 cactgcttgc ctgcgaccag tccaaccagt ttaattggtt atcctgacag taggcattgt
     5641 tattgccatg ctggctgtga ccatgttcgt caccggccag taacatcggc gtgccctggg
     5701 agagcaacaa cgttgttaac agggcatgaa tgctgtcgcg ccgccgttca accaggtcaa
     5761 gagtaccgcc taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc
     5821 gattttcttc tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg
     5881 taaaaccgtc atgcgcggtg acgagattaa tcgcggcact cggcagacga ccattacgtt
     5941 taaaaacatc gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat
     6001 gtagccagaa acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg
     6061 ggaaatttcc cacctgataa ccactaggag cgatatccca cggttcagca attaacttca
     6121 cctgcgagag cactgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact
     6181 ctggcgtacg gcccatgact gccgccagat caaagcggaa accatcgacg tggcaggttt
     6241 ctacccaata acgcaggcag gcgctggcat aatccaccac cgccggatga cttaaattga
     6301 gcgtgttgcc gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc
     6361 tacggttatc gatcccacgc agcgagaata acgggccgtc gaggtccagt tccgcactat
     6421 ggttgagcac gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat
     6481 cgcgaaactc atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca
     6541 tcgccaccgg gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac
     6601 tggcaaactg cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt
     6661 tgatcatcac cggatgcccg agggctttat aagtgccacg gatctcgacc gggatctccg
     6721 ggtgcaagta cgttaatcct ttgacatggg cttcataaat gatggtgctg ccccacggcg
     6781 tgcgcggcgg ggcgtcatct tcccagtcat agtgatcaac caccactacg catttcggcg
     6841 caatggcggc gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat
     6901 ctttaaactc cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa
     6961 agcgatgccc ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg
     7021 ggcgcgcatc cggcagataa ccgtgccaaa tgtcgccact gttccctggc aagtcatagc
     7081 gatgttcctg gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga
     7141 aaagtgtgaa gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa
     7201 tggcgagttg tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagcgt
     7261 caggcttagt gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc
     7321 attgcctgca ttactgccgt gatagtgcat ggaatcggta ttgaggattt cacgccattt
     7381 gcctggctga ttaatgccga agcgataatc atgacgcggc accggcgtaa agttactggc
     7441 aacgataatt tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt
     7501 gtcatccacc accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt
     7561 atggtggcgg taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt
     7621 atcgccgcct tccaacagat gccagtcgag gctggcgtca tggttccact cgcggccctg
     7681 ggcaaattcg ttccccatga acagcagttt cttgcccgga aatgcccaca tccagccata
     7741 gtaagcgcgc aggttagcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga
     7801 ttttttaccg tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt
     7861 gtagagcatc ccgaaggtca gtttatcgtg atgatactga cgataaatcg ggtcgagctt
     7921 catgtagtcc agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc
     7981 gcccatatcc tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac
     8041 cgcaccggaa acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc
     8101 aagattctcg cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc
     8161 acgataaatc atcgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat
     8221 ccagtaaagc gcgttaccga cgaggaagtt actgacttca cggcgaccat agttgtagat
     8281 cagcgtgttc cagtcctgat gatagccttc acgcggatcg ctgtgttcat acaagttcgt
     8341 gccatcaaat tcggcaagcg caaagtcatc agtcgggaag tggcctggca cccagtcgag
     8401 aatcacgttc agaccagctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt
     8461 accaaaacgg cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa
     8521 gggatgctcg ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg
     8581 caccagttga tcggccagct cgcggtagct caaccagaaa ttgttgtcgg tgtggcgacg
     8641 ccaggaaccc aggtgaactt cataaataga gattggcgca tcaaactgat tcgctttttt
     8701 gcgctcttca gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg
     8761 gcgcatttgc gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc
     8821 aatcatctcg tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccagat
     8881 gccgctctct ttacgcagtc gcatcgggtg acggcgaccg tcccagtagt tgaattgccc
     8941 aaccaccgag acccgacggg cgtttggagc ccagacagag aaacgcgtac ctgtgacgcc
     9001 atccatagta tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga
     9061 taatagccag gcatccattt cctggattag cggaccaaaa cggtaaggat catcaatcag
     9121 gttttgctga ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg
     9181 aatgacgccg ctaaagaatc cccgtgagtc gagacactcc agttttgcga gtttgcgccc
     9241 ggttttcggt tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag
     9301 tcccgcggtg gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat
     9361 tagcgcgtta atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg
     9421 tgtcacccca tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg
     9481 cgcagcactc tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc
     9541 atgtattcag ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga
     9601 aacatctgcg cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta
     9661 atgaataaag attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac
     9721 agcagctggt cgcccacggt aaaggctgac aggaactctg gtcccatatt cagcttacgc
     9781 aggcggccta ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata
     9841 gtgatttccc gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct
     9901 tcgacggtcg gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag
     9961 cgcaatgccc cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg
    10021 atcttgttgg tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt
    10081 ttgtcgatcc acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc
    10141 tcaccgctac gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg
    10201 gtcgcgagtt catctgccac atggccatac agatggccca tctgggttaa taactcacgc
    10261 atatgtcgcg caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca
    10321 agatcattgg cgaataaacc acccaacgac atcaacatca agcttacggt acagttaccg
    10381 ccaacaaaag tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg
    10441 tcaagaatga tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa
    10501 ccttgccatc cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga
    10561 caggtcacaa tgatatcgag ggcctttagc gcctccagat caaaggcatc ctgaagtgtg
    10621 ccagtggttc cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca
    10681 gggcgaatgg cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc
    10741 ataccgcgcc agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg
    10801 tgctgtataa atgtgccggt ctcctcttgg cacatctttc accatacaaa aagcagccaa
    10861 agtcgcaagt gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt
    10921 tgttagcacg cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat
    10981 ttctgcagca gttttattga tcctgattat ggatccgctc ggaaacctgc ctattttcat
    11041 gtccgtactg aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct
    11101 tattgctctc ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct
    11161 cagcctgcga gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat
    11221 taagatgatt ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc
    11281 atttatcgtg ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat
    11341 gttgttgtct catcagtacc cgaatcagat gggacatctg gtgattgctc tgctgctggc
    11401 ctggggcggc acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga
    11461 gaaaggggtg aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaactca
    11521 gatgttcctc gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa
    11581 gagcccggta gcgtcacaac taccgggcga acaaacttaa ctcaacagct ggaacgcaat
    11641 catcccaacg atggcaccaa cagtgccgag gatggtttcc atcatggtcc aagttttcag
    11701 cgtttcggct tcggtcgcgc cggtaaattt accgaacaac cagaaaccag cgtcgttaac
    11761 gtggctgaca acaatcgaac caccagcgat acaaatcgac agtgccgcca tttgcgcacc
    11821 agagtagttc agttgttcaa tgactggcat caccagtccc accgccgtta aacaggctac
    11881 ggtggcagac ccctgaatga tgcgcactgc agcagccagc acgaagcagg tgatagcaat
    11941 cggcaggccc atgccggtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag
    12001 cacctgtttg aacacgccgc ctgcaccaat caccagcaga atgatccccg ccggttgcag
    12061 cgcgtgaccg caaatctcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc
    12121 ataaatcgct accagacaag caaccagaat cgcggtaaac ggatgaccaa taaactcgaa
    12181 ccattcgtaa gcggtagagc cttctggcac aaaacgcgcg gcaatggttt tcagccccac
    12241 cagcaccagc ggcaacagga tcagcgacag gctgaatccg aaagatggca ttttaccttc
    12301 gccgagatgc ggttcgctga tgtcgtcagg aatatgcagc tcaacgtaac ggctgatgaa
    12361 gttaccccac agcggcccgg caataatcat tcccggaatt gccgcacaca ggccaatcag
    12421 gatcatccag ccaaagtcgg cattcatctg cgatgccagc agcattggcg ctggcccaag
    12481 caccaggaac gctgccgcgg ccgccacgcc tgcgaataat gggattacca gcttcaccag
    12541 gttcgtaccg gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa caatcgccac
    12601 ttcaaagaac agcggtagcg cacagaccag ccccgcaagg ccgatggcat aatgcgcgcg
    12661 gctgtgaccg aaggatttga gcattttgac ggcaatctga tcgactgcgc cggtttcata
    12721 taagatcttg ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggtgcc
    12781 tcccatccct ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc
    12841 agcccccatg gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa
    12901 aaacagcagc agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac
    12961 ttatttgcct tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc
    13021 gatatccacc accagtacat cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg
    13081 cgtcaccaac atttgggttt taaagaaatg gcctttgcgc gctttcaggc gactttcaat
    13141 cacatcaaaa tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa
    13201 gtcgcgatag tgctttttca gtgcagaaca gacgatcagc gacactttat tggtgcgctg
    13261 catagcaaac gcggagtcgt tcagcgcctg caaccagggt ttgcgatcgt cgtcattcag
    13321 cggttcgcca gacgccattt tttcgatatt gcgccgtgga tggaggaaat cgccatcaag
    13381 aaacgcggca tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcccga
    13441 tacgcccatc aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc
    13501 acaatacaag aattaaaaat gttacgggta actgttatcg gtaacattgt ccagccggac
    13561 aatatcagaa gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt
    13621 gtgggcttaa atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt
    13681 cacagattcg ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc
    13741 acgcggcgtc agcacgctcg caagtcgtgg ctccatcacc tgaccaatgt catgaccgtg
    13801 gaaaccggca atcgccatat cgtcaggaac ttttaacccc agacgctgac attcaaacgc
    13861 cgcgccgacc gccagatcat cattagtaca gaacacgcca tccagctgcg gatattcccg
    13921 ccgcgcctgg cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac
    13981 gctatatggc accaggcctg catccagcat cgcctgttcg tatcccttct gtttgatgat
    14041 agtacgttcg tcgagacgtg cgccgagata ggcaatgtgg cgatgcccgc gagcaataat
    14101 ggcggtagtc atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca
    14161 cggcgactgg ctgtccatca actccaccac cgggatcccc gccacttcaa tcatctttaa
    14221 ggtgcgcggc gtgtgggtac gttcggtgag gatcaggccg tcgatattcc aggagagcat
    14281 ggattcgaga cgttcttgct ccatttccgg tttataaccg tagtgcgcca gcatggtctg
    14341 ataaccgtgc gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt
    14401 ggtgagagaa ggtaacagga cgccaatcgc ccggctggta gcgttagaga ggatatcggg
    14461 cgcacgattg ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc
    14521 gacggaaacc tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac
    14581 acggtcagcc acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa
    14641 gagatagatt ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct
    14701 tgtttacaat tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc
    14761 aaaaccccgg aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg
    14821 caggcttcaa agatgccgtt gagttttaaa ccggcggcag atcgaacagt aacacttcgc
    14881 tgtcgctatc cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg
    14941 tcgaggcttt cacgccgtta atggtaacat tgccttttac cacctggatc cagacgcggc
    15001 gttcagcggc aatttgatgc accgactgct catctttcag caacgcccaa cgatacagtt
    15061 ccatatcctg atgcactttc agcgaaccat ctcgcgcatc cggcgagagc accagctgtt
    15121 tgccctgcac ggcatcgaag cgacgctgtt cataacgcgg cgtaatgcca ttttcttcag
    15181 gcatgatcca gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt
    15241 gacgaatacc cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat
    15301 tgcccatgct gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat
    15361 ctttatgcgg gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg ttaatcacgc
    15421 gcagcgcgga gaagcccata aagttcggat cgtaatagtt ggcaaaagag aaagtatgcc
    15481 aggagtccag ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca
    15541 tttgtattcg ccccctgaat gatttcgatg ggcttagtgt ggacccgatc tgcctgggat
    15601 gatagagggt gaaaattgac ccctctgttc aaaaaaattg aactattcga ggggccatca
    15661 aacttaattc gcgagagtca ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat
    15721 ttccagattg gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt
    15781 ttgatacaac gcatcataaa cgcgcccgta atcgcccatc tccggcttca tctcttctct
    15841 gaccgtcaca ccctcgtcat tgacatactc cagcacaccg accgaatcat ccgctgcgaa
    15901 tcccggttcg cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta
    15961 tttaataaac gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag
    16021 atggctggtt ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc
    16081 gtcaggattg gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc
    16141 gaacagagaa ataatctggt ccatcgtatg cacaccaagg ccatagaacg cgccatcctg
    16201 cggcagtcca ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc tttccacttc
    16261 gacaatctct cccagcttgc cgctttcaat cgcttttttc gccgtcagga agcaggaatc
    16321 aaagcgacga ttctgatatg gcgtgacggt cagccctttg cttttcgcca gtgcaaacag
    16381 ctctttcgcc tgcgcaagtg tcggagtgaa cgttttttcg accagcacat ttttcccggc
    16441 ttccagcgcg cgcttcgcgt actcgaagtg gctgtccgca tgggtgcaga caacaaccaa
    16501 cttaacatcg ggatcgttta gcacttcgtc gagatcgctg gtgaaatgga tatgagagta
    16561 aatgggagcc tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct
    16621 atccttgcgg ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat
    16681 aaaggcgcag ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg
    16741 caaagcggac gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa
    16801 attgagaacg aaagatcaaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc
    16861 aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgaaacaat
    16921 aataatcatt ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc
    16981 atttgaaagt caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg
    17041 agatagtaat acgccacgca gaaacacggg attacgaggc catcaggcag attcacgccc
    17101 agccggaggt gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg
    17161 agcgactcgc cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg
    17221 tgggccatct caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg
    17281 gtatctgtgt cgactctcgt tggaa
//
LOCUS       DADLSA010000082        16948 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.082, whole genome shotgun sequence.
ACCESSION   DADLSA010000082 DADLSA010000000
VERSION     DADLSA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16948
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.082"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(316..1512)
                     /gene="hemY"
                     /locus_tag="JN691_002631"
     CDS             complement(316..1512)
                     /gene="hemY"
                     /locus_tag="JN691_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAY7244045.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1515..2738)
                     /gene="hemX"
                     /locus_tag="JN691_002632"
     CDS             complement(1515..2738)
                     /gene="hemX"
                     /locus_tag="JN691_002632"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY7244046.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPQGDTPAAAPQGE"
     gene            complement(2760..3500)
                     /gene="hemD"
                     /locus_tag="JN691_002633"
     CDS             complement(2760..3500)
                     /gene="hemD"
                     /locus_tag="JN691_002633"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAY7244047.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3497..4438)
                     /gene="hemC"
                     /locus_tag="JN691_002634"
     CDS             complement(3497..4438)
                     /gene="hemC"
                     /locus_tag="JN691_002634"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAY7244048.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVVLLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            4825..7371
                     /gene="cyaA"
                     /locus_tag="JN691_002635"
     CDS             4825..7371
                     /gene="cyaA"
                     /locus_tag="JN691_002635"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAY7244049.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7411..7731)
                     /gene="cyaY"
                     /locus_tag="JN691_002636"
     CDS             complement(7411..7731)
                     /gene="cyaY"
                     /locus_tag="JN691_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAY7244050.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7779..9092)
                     /locus_tag="JN691_002637"
     CDS             complement(7779..9092)
                     /locus_tag="JN691_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HAY7244051.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYPNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9089..10554)
                     /locus_tag="JN691_002638"
                     /pseudo
     CDS             complement(9089..10554)
                     /locus_tag="JN691_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            10743..10946
                     /locus_tag="JN691_002639"
     CDS             10743..10946
                     /locus_tag="JN691_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7244052.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            10983..11807
                     /gene="dapF"
                     /locus_tag="JN691_002640"
     CDS             10983..11807
                     /gene="dapF"
                     /locus_tag="JN691_002640"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAY7244053.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            11804..12511
                     /locus_tag="JN691_002641"
     CDS             11804..12511
                     /locus_tag="JN691_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAY7244054.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLVRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12508..13404
                     /gene="xerC"
                     /locus_tag="JN691_002642"
     CDS             12508..13404
                     /gene="xerC"
                     /locus_tag="JN691_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAY7244055.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13404..14120
                     /gene="yigB"
                     /locus_tag="JN691_002643"
     CDS             13404..14120
                     /gene="yigB"
                     /locus_tag="JN691_002643"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAY7244056.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14204..16366
                     /gene="uvrD"
                     /locus_tag="JN691_002644"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14204..16366
                     /gene="uvrD"
                     /locus_tag="JN691_002644"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAY7244057.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16513..16944)
                     /locus_tag="JN691_002645"
     CDS             complement(16513..16944)
                     /locus_tag="JN691_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244058.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         3894 a         4418 c         4522 g         4114 t
ORIGIN      
        1 attatctcat aacaagtgtt aagggatgtt atttcccgat tctctgtggc ataataaacg
       61 agtagatgct cattccatct cttatgttcg ccttagtgcc tcataaactc cggaatgacg
      121 cagagccgtt tacggtgctt atcgtccact gacagatgtc gcttatgcct catcagacac
      181 catggacaca acgttgagtg aagcacccac ttgttgtcat acagacctgt tttaacgcct
      241 gctccgtaat aagagcaggc gtttttttat gcatcagaaa ggtcccggag gtgctttcct
      301 ccgggtgaga aggaactact gtggcgggtt attctgcaac gttaacatca aaccgtcgcg
      361 acgcatcgct gcagcttctt ccggcttgtg cagtctgtcc agcgcgtcgg caagccatgc
      421 gtaatcgtag gcgtccggac gttgtttcag cgccgcgcgg aaggcgagcg atgcttcctg
      481 ccattcaccg tgcttcatca gtgactgacc caatgtgctc cacaacagcg ggcgatcacc
      541 gacgtttttg atttgctggc gtagcacttt ttccagctgt tccgggttat tggttttcag
      601 acgcgggatc ggcagcagta ggcgatcgtc atactggcgt ttcaggccgt cgatgataat
      661 ttgctgggca gtatcatgat cgtcacattc aataagatgt tccgccattg ccacctgcaa
      721 cgctacctga tgacgcgttt tccggctttg gtttttccac cagttacgca aaccttcact
      781 accgttatcg gcacgcgcct gatccatcag gccaatccat gcctgttgtt ccagcattgc
      841 acgatgttct tcatcaccaa catgggcttt cgccattgat gggatgatat ccagcagaga
      901 actccatgcc cctgtgcgga tatacgcctg ttccgccaga cgtaatactt caggatggcg
      961 tggcgtaact tccagcagct tatccacgcc gtggcgtgca gcatggtttt cattacgggc
     1021 cagttgcaga cgtacacggg tgatttctac cggaatggtg tcattgccgg ccagctccgc
     1081 tgcgcgttcc agatgttggt tggcgcgtgc ttcatcacca cgttgttgcg cggcttcggc
     1141 agccagtaga tagttcacca ccggttgttc agcgtgatcg gcatttttcg ccatcagctt
     1201 ttcaacttgc tgataatcac cttccgccag tttcagcagc gcctgttcgg tctgcttacg
     1261 cgcacggcga cgcttgcgtc cgacaaacca tccacgggtg tgcgcgccag tgcggaagat
     1321 ccgccgcagt agccactcaa tggcaaacag cactaccatc gccagaatca atatgatcgc
     1381 caggcccgtg acgctggttt cgatattgta gttgtcggtc tggatcagca cataaccctg
     1441 atgaccggca atcatcgggc caaccactat ccccgcaatc agcaaaacaa agagtaataa
     1501 cacttttagc atggttattc tccttgcggc gcggctgccg gagtatcacc ttgcggtgca
     1561 ggtgcaggtg caggtgcagg tgcaggtgca ggtgcaggtg caggtgcagg tttagcttcc
     1621 gttgtccccg ctgccggttg tgccagcagg ttacgcacgc gagtctgcat cagtttttcc
     1681 agcatcgcct ggctttgcag ggtttccgga aggtccatcg agatattttg ctggcttaac
     1741 tggtccacct cgtcgaggaa cgctttggtg gtggcatcat cagtatcgta gtaagcacgt
     1801 acccaggtgg agacgttctc cagcgcctgg cgataagtct cttcctgatg acgcggtaca
     1861 gcttgtgctg cgaccagcag gcgagagcga atattttcgc gcagatagat atcctgattt
     1921 ggcgctaaca gcggtacggc ggtgtcatca cgacggcgaa tcgtaatgaa gttgtccata
     1981 aagttctgcc agcttttttg cagattgata cgccattcgc tgatggaact ggaaagctct
     2041 tcaccgtctg aatccatcgg cgaaccatcg ctgtcattat cggccagacg caggttatct
     2101 acctgatttg aaagctgatt aagcttaagg atgatgccgt cataatccac ctgcgatact
     2161 gcagaaaggc tggcgatatc atcggtaatt gcccgacgaa cggtaatcag actcgggtca
     2221 ttcatatccg ccaggctggc gtctgcactt ttcagcaacg ctgcagaggt cgtgacgtcc
     2281 tgatcgctcc acagcttccg tccggcgagt ttcaccagaa aatcggcctg agccagcagc
     2341 caggttttag catcgctgcc ggaaatggtg gcgatctttt gttggacttc atccaactgt
     2401 tttgccagcg tttcttgctg acgattcgcc tgcttaagct gcgcagcttg ttgcttaata
     2461 atgccttcca gctcggcttt ttggctctcc tgggcttttt gcaatgccgt cagttggtta
     2521 gccagggcat cgctggtggc ggtctgattg acggcctgtt gtttacccca gccatacaaa
     2581 ccgacacccg ccgccagagc aatagcgata gccaccgcgc tgagaatcaa tgcagtattg
     2641 ttcttactct ttttttctgt tgcgacaggt tgtgacgtgg tgtccacggc ctccctagtc
     2701 tcttcaacca cggcggaggt tttttcttgt tccgtcatta tggcttcctg ttatgagagt
     2761 tattgtaatg cccgtaaaag cgcatcgttg tcagcgttat cggcgacctt aatgtcttgc
     2821 cagcccagtt cccgggcgag tttcgccaaa cgctcactga cgaccaatag tcgacagtgt
     2881 agtaaccagt gctcacgata ccattgtggg atcagcgacc agagttgctg caacatttca
     2941 ccgctggtaa caacgaccat cgtcacctcg cgggattgcc agcgcatcgc ttcttctgca
     3001 ccatcgtaat ggattgcgca tcgttgataa cattcacaaa aagtgacctc agcaccgcgc
     3061 accgtcaggg tatccccaat tagctcacga ccgccattgc cacgtaatat cagcgcacgt
     3121 ttgcccgcaa tattttgtaa ttcaggtaat tgtagcaaga cttcgctgat ttcccgatcc
     3181 tgtgggtaga gaatcttctg tccgcttacg gtatgtagcg ccagtgcggt ggtgcgtcca
     3241 atggcgaaat aagtaggtag ttggggccat ttacgatctt gctgatgcag ctgtgattgg
     3301 gcaaaagcaa ccgcgtgttg cgagagggca aacaacagat cgctctcccc cagcgctgcc
     3361 agttgatcag caagttgcgg taattgtcga cccggagaaa actcaatcag cggaaaatgc
     3421 caggccacct gccccagtgt gcgcagacgg ctcactaact cttctccagc gggagacggg
     3481 cgggtgacca ggatactcat gccggggcct ctccgttata gacttcagcg aggatctcgc
     3541 gcgcgccgtt attcagtagc tcttctgcca gcgaaatccc catttgttcg gcatcttgcg
     3601 gcgcaccgcg gcgttcaccg cgaataatct gcgaaccgtc cggcgcgccg accagcgcac
     3661 gtagccagat ttcgccatca ataagctcgg cgtagctacc aattggcacc tgacatccgc
     3721 cttcgagacg ggtattcatg gcgcgttctg cggtaacgcg cagtgcagtt tcgtggtgat
     3781 tcagcgcggc aagcagctcg cgagtgcgtg tatcatcaag gcggcattca ataccgactg
     3841 caccttgtcc taccgccgga agagaaatct cgggtggtaa cgcggcgcga atgcgggact
     3901 ccagacctaa acgttttagt ccggctacgg caagaatgat ggcatcgtat tcgccgttat
     3961 ctagtttgct caggcgagtg ccgacgttgc cgcgcaggga gcggataatc agatccgggc
     4021 ggcgttcagc cagttggcac tggcgacgta aactggacgt cccgacgata ctgcctgctg
     4081 gtaacgcatc cagactgtca tagttattgg acacaaaggc atcgcgagga tcttcacgct
     4141 cgcaaatagt gaccagcccc agaccttgcg ggaattcaac cggcacatct ttcattgaat
     4201 gtacggcgat atcagcgcga ttctccagga gcacgacttc cagctctttt acaaataagc
     4261 cttttccgcc tactttcgcc agcggcgtat caagaatcac atcgccgcgc gtcaccatcg
     4321 gtaccagttc aacgaccagg cccggatggc tcgccatcaa cttgtctttg acatagtgtg
     4381 cctgccagag tgcaagtggg ctttggcgtg tggcaattct taaaacattg tctaacatgc
     4441 ttgttaccgt cattatcatc cgtggtccat cctaacatcc ttgccagagt gatgtcagtg
     4501 ttgtggtgaa acgtagacgc ctgcgcaaac cgtaaaatga ggtctggcag tggatcctga
     4561 caggcgtttc acgccgtcgt aataaggaat ttacagagaa taaacggtgc tacacttgta
     4621 tgtagcgcat ctttctttac ggtcaatcag caaggtgtta aattgatcac gttttagacc
     4681 attttttcgt cgtgaaacta aaaaaaccag gcgcgaaaag tggtaacggt tacctttgac
     4741 atacgaaata tcccgaatgc cgcgtgttac cgttgatgtt ggcggaatca cagtcatgac
     4801 gggtagcaaa tcaggcgata cgtcttgtac ctctatattg agactctgaa acagagactg
     4861 gatgccataa atcaattgcg tgtggatcgc gcgcttgctg ctatggggcc tgcattccaa
     4921 caggtctaca gtctactgcc gacattgttg cactatcacc atccgctaat gccgggttac
     4981 cttgatggta acgttcccaa aggcatttgc ctttacacgc ctgatgaaac tcaacgccac
     5041 tacctgaacg agcttgaact gtatcgtgga atgtcagtac aggatccacc gaaaggtgag
     5101 cttccaatta ctggtgtata caccatgggc agcacctcgt ccgtagggca aagttgttcc
     5161 tctgacctgg atatctgggt ctgtcatcaa tcctggctcg atagcgaaga gcgccagtta
     5221 ctacaacgta aatgcagcct gctggaaagc tgggccgcct cgctgggtgt ggaagtcagc
     5281 ttcttcctga ttgatgaaaa ccgcttccgt cataatgaaa gcggcagcct ggggggcgaa
     5341 gattgtggct ccacccagca tatactgctg cttgacgaat tttatcgtac tgccgtgcgt
     5401 ctcgccggta agcgtattct gtggaatatg gtgccgtgcg acgaagaaga gcattacgac
     5461 gactatgtga tgacgcttta cgcgcagggc gtgctgacgc caaatgaatg gctggatctc
     5521 ggtggcttaa gctcgctttc tgctgaagag tactttggtg ccagcctttg gcagctctac
     5581 aagagtatcg attccccata caaagcggtg ctgaaaacac tgctgctgga agcctattcc
     5641 tgggaatacc cgaacccacg tctgctggcg aaagatatca aacagcgttt gcacgacggc
     5701 gagattgtat cgtttggcct cgatccatac tgcatgatgc tggagcgtgt tactgaatac
     5761 ctgacggcga ttgaagattt cacccgtctg gatttagtac gtcgctgctt ctatttaaaa
     5821 gtgtgcgaaa aactcagccg tgaacgcgcc tgtgtaggct ggcgtcgcgc agtgttgagc
     5881 cagttagtga gcgagtgggg ttgggacgaa gctcgtctgg caatgctcga taaccgtgct
     5941 aactggaaga ttgatcaggt gcgtgaggcg cacaacgagc tgctcgacgc gatgatgcag
     6001 agctaccgta atctgatccg ctttgcgcgt cgcaataacc ttagcgtctc cgccagtccg
     6061 caggatatcg gcgtactgac gcgtaaactg tatgccgcgt ttgaagcatt gccaggtaaa
     6121 gtgacgctgg taaacccgca gatttcaccc gatctctcgg aaccgaatct gacctttatt
     6181 tatgtgccgc cgggccgggc taaccgttca ggttggtatc tgtataaccg cgcgccaaat
     6241 attgagtcga tcatcagcca tcagccgctg gaatataacc gttacctgaa taaactggtg
     6301 gcgtgggcat ggtttaacgg cctgctgacc tcgcgcaccc gtttgtatat taaaggtaac
     6361 ggcattgtcg atttgcctaa gttgcaggag atggtcgccg acgtgtcgca ccatttcccg
     6421 ctgcgcttac ctgcaccgac accgaaggcg ctctacagcc cgtgtgagat tcgccatctg
     6481 gcgattatcg ttaacctgga atatgacccg acagctgcgt tccgcaatca ggtggtgcat
     6541 tttgatttcc gtaagctgga tgtcttcagc tttggcgaga atcaaaattg cctggtaggt
     6601 agcgttgacc tgctgtaccg caactcgtgg aacgaagtgc gtacgctgca cttcaacggc
     6661 gagcaatcga tgatcgaagc cctgaaaact attctcggca aaatgcatca ggacgccgca
     6721 ccgccagata gcgtggaagt cttctgttat agccagcatc tgcgcggctt aatccgtact
     6781 cgcgtgcagc aactggtttc tgagtgtatt gaactgcgcc tttccagcac ccgccaggaa
     6841 accgggcgtt tcaaggcgct gcgcgtttct ggtcaaacct ggggcttgtt cttcgaacgc
     6901 ctgaatgtat cggtacagaa gctggagaac gccatcgagt tttatggcgc tatttcgcat
     6961 aacaaactgc acggcttgtc agtgcaggtt gaaaccaatc acgtcaaatt accggcggtg
     7021 gtggacggct ttgccagcga agggatcatc cagttctttt tcgaagaaac gcaagacgag
     7081 aatggcttta atatctacat tctcgacgaa agcaaccggg ttgaggtgta tcaccactgc
     7141 gaaggcagca aagaggagct ggtacgtgac gtcagtcgct tctactcgtc atcgcacgac
     7201 cgcttcacct acggctcaag cttcatcaac ttcaacctgc cgcagttcta tcagattgtg
     7261 aaggttgatg gtcgcgaaca ggtgattccg ttccgaacaa aatcgattgg caacctgccg
     7321 cccgccaatc aggatcacga tacgccgcta ttacagcagt atttttcgtg atgagcgtgc
     7381 cggaaagcga ggcttatccg gcatgcaatc ttagcggaaa ctgactgttt cacccgcctg
     7441 ttgcgtcgcc gcctgttcca gcaaatccca gaaggtttcg ccgctgcgat cgcaaatcca
     7501 ttcatcgcct ttcaggtcaa aatggtagcc gccctgtttg gttgccagcc acacctggtg
     7561 cagcggctcc tggcggttaa taatgatttt gctgccgttt tcaaaggtaa tggtcagtac
     7621 gccgccgttg atttcgcagt cgatatcgct gtcgccatcc ctgtcgtcca gacgctcttc
     7681 aatggtcagc cacagttgat cagccaggcg atgaaattca ctgtcgttca ttgttgtatc
     7741 ctgtttttaa gtgatggcgg cagtatagcg gcatggggtc agggcttcaa agtttgcacc
     7801 tctgctgctg cgttccggca tgattcatcc gtcaccggaa taatgatgtc tctgttttga
     7861 acttcacgta cgttctcctc tctgacacga gttttcgccg ggcaggggga gggcgtaatc
     7921 cttacctctg tatcggtcca tgtataaaca ttgcgatcgt cataagcgaa gcgatcgttt
     7981 ttagttagcg gaccgttaac tggaataatt tgcaagctct ttgaccgtaa ttggcggata
     8041 aagtaagcat gctgtttgcc gctattaaat gagcgatcaa tgaccaggtc tggcgtatcc
     8101 agcgggatta tctcaagctg gtcttcacca aatgtcgata attttgttac atacagccga
     8161 tctttatatt gcgcaatatt gccagtaatc agatggaatt gttcaggttc aatacctggt
     8221 aggtctatcc acttgctcca ctgacggtcg ggcgtgagct gtttacgcca gcgaacgcga
     8281 ctttccccta cttcaaaaga gacgtcatca tctacgggta tcgctttgcc tgataattga
     8341 ccaaagggat aacgatgcag acccttattg tcgcgaaaaa ccagcagtga atgaggtatc
     8401 cagcgaatta tctcaaatga gtctgcattc gccgtaaggt gaacgccatt taaataaata
     8461 ttctgcccac tgcggataag cagatcattg ggatatgaat ttctgtttac actcataaga
     8521 cgtggattaa taccccacca tcttttttct aatacagtaa catcactgct gctcccctga
     8581 cgtcgaccgg acaagtagag atttttacta tccaccagcg tggtagagtt accttcgata
     8641 gcttttagtg tcgccagatc aacacgtgac gcgccgctat tactttcggt gcgctgtccg
     8701 tcaaaataga ggctatattt atcgaccgcg aagcggccca tagcctgaaa gctggcaata
     8761 tcgacagtgg gtgttcccgg tggattgcta acgattttgc cacgccagag aataacgcgg
     8821 ccgtctgtca cccagtggta ttcctcattt tctatgctta tttcatcgga aggtatataa
     8881 cccgttttcc cagagatatt tttcacaagg cgcaaatcgg ccctgctcat ctccggaaac
     8941 tgggcaatga ttttcttatc cccccaataa cctttaccgt aagacataac accgtgatca
     9001 ttgtaatagt cgaaccctat ggcctgtaca gcaggcgtca gaaatgtgtg tcccaacagg
     9061 atgagtgcaa ctgtgattat ttgacccttc attttgtcgc ctcattgcgc ggggcaggaa
     9121 taaattcgcc gtcatcagta atttttccgt ccagatatcc cgttttgtaa tttacgccat
     9181 agccatatcc cgaatgttgt tccttccaga ttaagcgaac ttgtttcgga tcagcggtgc
     9241 ggtatcgcca gagctgcgcg ccatcaaagc tgtaaatgta gcggttatcg cgggcatacc
     9301 atctgcgcaa aaagggatgg tcattaagat gggcttcgcg ttcatccgga gaacggttat
     9361 ccagtaagat cagcggtccg tcagattcaa acacgtgtaa accggaatgg tatggccact
     9421 cttcggcacg cgtaagtaaa tagccgtggc gtttcccggc gttgaaacgt ttgttgataa
     9481 taagattggg atcatcaagt ttcacgatct ccagcttacg gtcaccagac tctgtttcga
     9541 tgatggtata aagcgagtcc tgatactggg cgacagcatc actgatgggt tgaaactgct
     9601 ccggatcgag tccgggtaat tcttcctgtt tacaagcagg tcctttacgc cagaacactt
     9661 tcttttccag cagagaaaaa tccagacagt gctttgttaa atcttcatcc cacgccagat
     9721 tttccttgtc gaggacttta cgctgcagcc cgtttttatc acgccaggtc aagaatgagc
     9781 cgggcatcca gcgcacgacg gaaaaggtgt ccgggtcggc atcaagctgt tcgccgttga
     9841 gcaggatttt ggtccgtgta cgagccaggg tatcccaggg gccaaatggt acggcaatgc
     9901 aggggttaaa cgtggtggaa aactttcctc gctgatccta ggacttctgc gccagcacac
     9961 ggaaactctc ggggtcagcg aggcgatgcc cattgatata caagaaatta gcgtcacgta
    10021 atatcagccc cagtaagtca ggatcccagg gaggccggaa ttccacctga tgcagcgttt
    10081 tgatatccaa gctatttcca ccaccattgt catcggtgcg cttgccttca aaatagagac
    10141 tgtgtttatc tgcggcaaaa tcaccccatg ccttgaacga ggcgacatcg acgggaggtg
    10201 tgccgggagg gttttgcaca acttcacccg cgtagaggat attgcgacca tcgctatgcc
    10261 atgaataatg aggataccag gcctgtgcgc cctcatagag gtagtcggtt gtcaaatcag
    10321 tcagaggcgg ttctttatcc cattcgtcta gtgttgtcgg gtctattaat aaattgggta
    10381 accgacgcaa actcttgtaa ttcacgtcag gtagagaaag atttgtttct ttataagggc
    10441 gcaggtacac cacgcctttt ccctgtttct ggtaaatatt atgagccgac atccccatgc
    10501 cgacgataaa aacaatgaac aagccaacaa acaaaccaca ttgcgatagt gcataaagcc
    10561 atcctggcgc gaggtgttga tcacgaaact gccagcagaa cataaatcct cacgagtaag
    10621 cgttatactc gcagcatttc ctcacttttc agacttcata aagagtcgct aaacgcttgc
    10681 ttttacgcct tctcctgcga tgatagaaag cagaaagcga tgaactttac aggcaatcca
    10741 taatgaaaaa cgtgtttaag gcactcactg tattacttac tctcttcagc ctgacgggct
    10801 gcggtctgaa aggtccgctc tatttcccgc ctgcagataa aaacgcaccg ccgccgacca
    10861 aaccggtaga gacgcaaacg caatccacgg tgccggataa aaacgatcgc gccactggcg
    10921 atggtccatc ccaggtgaat tactaaaagt cagtttctgt acccgcgtga ttggagtaaa
    10981 tgatgcagtt ctcgaaaatg catggccttg gcaacgattt tatggtcgtc gacgcggtaa
    11041 cgcagaatgt ctttttttca ccggagctga ttcgtcgcct ggcggatcgg cacctggggg
    11101 tagggtttga ccagctgctg gtggttgagc cgccgtatga tcctgaactg gattttcact
    11161 atcgcatttt caatgctgat ggcagtgaag tggcgcagtg cggtaacggt gcgcgctgct
    11221 ttgcccgttt tgtgcgtctg aaaggactga ccaataagcg tgatatccgc gtcagcaccg
    11281 ccaacgggcg gatggttctg accgtcaccg atgatgatct ggtccgcgta aatatgggcg
    11341 aacccaactt cgaaccttcc gccgtgccgt ttcgcgctaa caaagcggaa aagacctata
    11401 ttatgcgcgc cgccgagcag acaatcttat gcggcgtggt gtcgatggga aatccgcatt
    11461 gcgtgattca ggtcgatgat gtcgataccg cggcggtaga aacgcttggt cctgttctgg
    11521 aaagtcacga gcgttttccg gagcgcgcca atatcggttt tatgcaagtg gttaagcgcg
    11581 agcatattcg tttacgcgtt tatgagcgtg gggcaggaga aacccaggcc tgcggcagcg
    11641 gcgcgtgtgc ggcggtggcg gtagggattc agcaaggttt gctggacgaa gaagtacgcg
    11701 tggaactccc cggcggtcgt cttgatatcg cctggaaagg tccgggtcac ccgttatata
    11761 tgactggccc ggcggtacat gtctacgacg gatttattca tctatgaagc aaccagggga
    11821 agaactgcag gaaacactca cggagcttga tgaccgggcg gttgtcgatt atctgattaa
    11881 aaatcctgag ttttttatcc gcaatgcgcg cgcagtggaa gcgatacgtg tgccgcatcc
    11941 ggtacgcggc accgtttcgt tggtcgagtg gcacatggcc cgcgcacgta atcacattca
    12001 tgttctggaa gagaacatgg cgttgttgat ggagcaggct atcgccaacg aagggctgtt
    12061 ttatcgcctg ctctacctgc agcgcagtct caccgccgcc agcagtctcg acgatatgct
    12121 ggtgcgcttt caccgctggg cgcgcgatct cggcctggct ggtgcgagtc tgcgcctgtt
    12181 tccagatcgc tggcgcttag gtgcgccgtc gaaccacact catctggcat taagccgtca
    12241 gtctttcgaa ccgctgcgta ttcagcgttt ggggcaggaa cagcactatc ttgggccgct
    12301 taacggaccg gagctgctgg tggtgctacc ggaagcgaaa gcggtgggat cggtggcgat
    12361 gtcgatgctg ggaagcgatg ctgatttggg tgttgtgctg tttactagtc gcgatgccag
    12421 tcactatcaa caagggcagg gaacgcagtt acttcatgaa attgcgctga tgttgccgga
    12481 gcttctggag cgttggattg aacgcgtatg accgatttac acaccgatgt agaacgctac
    12541 ctacgttatc tgagcgtgga gcgtcagctt agcccgataa ccctacttaa ctaccagcgt
    12601 cagcttgagg cgatcatcaa ttttgccagc gaaaacggcc tgcaaagctg gcaacaatgc
    12661 gatgcagcga tggtacgcaa ttttgctgta cgcagtcgcc gtaaagggct gggagcagca
    12721 agtctggcgt tacggctttc tgcgctacgt agcttttttg actggctggt cagccagaac
    12781 gaactcaaag ctaacccggc gaaaggagtt tcggcaccga aagcgccgcg tcatctgccg
    12841 aaaaatatcg acgtcgacga tatgaatcgg ctgctggata ttgatatcaa cgatcccctc
    12901 gctgtacgcg accgtgcaat gctggaagtg atgtacggcg cgggtctgcg tctttccgag
    12961 ctggtggggc tggatatcaa acacctcgac ctggagtccg gcgaagtgtg ggtgatgggg
    13021 aaaggcagca aagagcgccg cctgccgatt ggtcgcaacg ctgtggcgtg gattgagcac
    13081 tggcttgatt tgcgcgacct gtttggtagc gaagacgacg cgctttttct gtcgaaactg
    13141 ggcaagcgta tctccgcgcg taatgtgcag aaacgctttg ccgaatgggg cataaaacaa
    13201 gggctgaata atcacgttca cccacataaa ttacgtcact cgttcgctac gcatatgctg
    13261 gagtcgagcg gcgatcttcg tggtgtgcag gagctactgg gtcatgccaa cctctccacc
    13321 acgcaaatct atactcatct tgattttcaa caccttgcct cggtgtacga tgcggcgcat
    13381 ccacgcgcca aacgggggaa ataatgcgtt tttaccggcc tttggggcgc atctcggcgc
    13441 tcacctttga cctggatgat accctttacg ataaccgtcc ggtgattttg cgcaccgagc
    13501 gagaggcgct tacctttgtg caaaattatc atccggcgct gcgcagcttc cagaatgaag
    13561 atctgcaacg cctgcgccag gcggtacggg aagcggaacc cgagatttat cacgacgtga
    13621 cgcgttggcg ttttcgttcg attgaacaag cgatgctcga cgccgggctg agtgccgaag
    13681 aagccagtgc aggcgcacac gcagcaatga ttaactttgc caaatggcgc agccgaatcg
    13741 acgtcccgca gcaaactcac gacaccttaa aacagctggc gaagaaatgg ccgctggtgg
    13801 cgatcaccaa cggtaacgcc cagccggaac tgtttggttt gggggattat tttgagtttg
    13861 tgctgcgcgc tggcccgcac gggcgctcaa aaccgttcag cgatatgtac tttttggctg
    13921 cggaaaaact caacgtgccg atcggcgaga tcttacatgt tggggacgat ctcaccactg
    13981 acgtgggtgg ggcaattcgc agcggaatgc aggcttgttg gatcagaccg gaaaatggcg
    14041 atctgatgca aacctgggac agccgtttac tgccacatct ggaaatttcc cggttggcat
    14101 ctctgacctc gctgatataa tcagcaaatc tgtatatata cccagctttt tggcggaggg
    14161 cgttgcgctt ctccgcccaa cctattttta cgcggcggtg ccaatggacg tttcttacct
    14221 gctcgacagc cttaatgaca aacagcgcga agcggtggcc gcgccacgca gcaaccttct
    14281 ggtgctggcg ggcgcgggca gtggtaagac gcgcgtactg gtgcatcgta tcgcctggct
    14341 gatgagcgtg gaaaactgct cgccatactc gattatggct gtgacgttta ccaacaaagc
    14401 ggcggcggag atgcgtcatc gtatcggaca actgatgggc accagccagg gcggcatgtg
    14461 ggtaggcacc ttccacgggc tggcgcaccg cctgctgcgt gcgcaccata tggacgccaa
    14521 tctgccgcag gatttccaga tcctcgatag cgaagaccag ctacgcctgc ttaagcgtct
    14581 gatcaaagcc atgaacctcg acgagaagca gtggccgccg cgccaggcaa tgtggtacat
    14641 caacagccag aaagatgaag gcctgcgtcc gcatcatatt caaagctacg gtaatccggt
    14701 ggagcagacc tggcagaagg tgtatcaggc gtatcaggaa gcgtgcgatc gcgcgggcct
    14761 ggtggacttc gccgagctgc tgctgcgcgc tcacgaactg tggcttaaca agccgcatat
    14821 cctacaacac taccgcgaac gttttaccaa tatcctggtg gacgaattcc aggataccaa
    14881 caacattcag tacgcgtgga tccgcctgct ggcgggcgac accggcaaag tgatgatcgt
    14941 cggtgatgac gaccagtcaa tctacggctg gcgcggggcg caggtggaga atattcagcg
    15001 tttccttaat gatttccccg gtgccgaaac tattcgtctg gagcaaaact accgctctac
    15061 cagcaatatt ctgagcgccg ctaacgccct gattgaaaac aataacgggc gtctgggtaa
    15121 aaaactgtgg accgatggcg cggacggtga gcctatttcc ctctattgcg cttttaacga
    15181 actcgatgaa gcgcgttttg tggttaaccg catcaaaacc tggcaggaca acggcggggc
    15241 gcttgccgag tgcgccattc tctaccgcag taacgcccag tcgcgtgtgc tggaagaagc
    15301 actgttgcag gcgagtatgc cgtaccgtat ttacggcggg atgcgcttct tcgaacgcca
    15361 ggaaatcaaa gatgcgctct cgtatctgcg cctgattgcc aaccgtaacg acgacgcggc
    15421 ctttgagcgt gtggtgaata cgccaacgcg gggtattggt gaccggacgc tggacgtggt
    15481 acgtcagaca tcgcgcgatc gccagttaac actctggcag gcatgtcgtg aactgttgca
    15541 ggaaaaagcc ctcgccggac gtgctgccag cgccttacag cggtttatgg aactgatcga
    15601 cgccttagcg caggaaactg ccgatatgcc gctgcatgta cagactgacc gggtaattaa
    15661 agactccggc ctgcgtacca tgtacgagca ggagaagggc gaaaaaggtc agacgcgtat
    15721 cgaaaactta gaggaactgg tgacggcaac gcgccagttc agctacaacg aagaagacga
    15781 agatttaatg ccgctgcagg cgttcctatc ccatgcggcg ctggaagcag gcgaagggca
    15841 ggcggatacc tggcaggatg cggtacaact gatgaccatg cactcggcga aaggtctgga
    15901 gttcccgcag gtgtttatcg tcggtatgga agagggcatg ttcccaagcc agatgtcgct
    15961 ggatgaaggc gggcgtctgg aagaagaacg ccgtctggcc tacgttggcg taacccgcgc
    16021 gatgcagaaa ctgacgctga cctacgcgga aactcgccgt ctgtatggca aagaggttta
    16081 ccatcgcccg tcgcgcttta ttggcgagtt gccggaagag tgtgtggaag aggtgcgcct
    16141 gcgcgccacg gtaagccgcc cggtcagcca tcagcggatg ggtacgccga tggtcgagaa
    16201 cgacagcggc tacaagctcg gccagcgtgt acgccacgct aagtttggtg aaggcaccat
    16261 cgtcaatatg gaaggcagcg gtgaacatag ccggttgcag gtggcattcc agggccaggg
    16321 aatcaaatgg ctggtggcag cttacgcccg gctggagtcg gtgtaacgtt gccggatgcg
    16381 gtgctgcgca ccttatttgg cctaaaaaat cattcagatt caataaattg caacgtcatg
    16441 taggccggat aaggcgttta cgccgcatcc ggcatcagca gcatatttag tatctgacac
    16501 aaagctatca ttctaacctt tccgctcgac ggaaatcatg gtgacaaaag gatagcgttg
    16561 ccacgggatt gccccgcctt tcatatacat atgtgaaatc gtgccatcaa gataaagcag
    16621 ctgctcaacg ttcagtttcg ctttggcata acaggcaaaa tcataaaaat ttgtcgcctg
    16681 ctggctcaac aaaaacacgg cattcccatg tttattaatc ccaacaccgt tacgaatttt
    16741 acgtgaggcg acgttgggat gaatacgcgg attaattaca ccgttttcca tcaacattgg
    16801 tcctgactgc accgcaaact gaatatcttt actggcttta aaagcatcca gacgaacgat
    16861 gccgacttta tctcccgcga cataaaacac gccgccagga cggataaaga aattcccttc
    16921 acctgaagcg agatttaacg ccaccttc
//
LOCUS       DADLSA010000083        16935 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.083, whole genome shotgun sequence.
ACCESSION   DADLSA010000083 DADLSA010000000
VERSION     DADLSA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.083"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            190..1323
                     /gene="fepE"
                     /locus_tag="JN691_002646"
     CDS             190..1323
                     /gene="fepE"
                     /locus_tag="JN691_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY7244059.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(1320..2135)
                     /gene="fepC"
                     /locus_tag="JN691_002647"
     CDS             complement(1320..2135)
                     /gene="fepC"
                     /locus_tag="JN691_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY7244060.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(2132..3124)
                     /gene="fepG"
                     /locus_tag="JN691_002648"
     CDS             complement(2132..3124)
                     /gene="fepG"
                     /locus_tag="JN691_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HAY7244061.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            complement(3121..4125)
                     /gene="fepD"
                     /locus_tag="JN691_002649"
     CDS             complement(3121..4125)
                     /gene="fepD"
                     /locus_tag="JN691_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HAY7244062.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            4236..5486
                     /gene="entS"
                     /locus_tag="JN691_002650"
     CDS             4236..5486
                     /gene="entS"
                     /locus_tag="JN691_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HAY7244063.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            complement(5490..6446)
                     /gene="fepB"
                     /locus_tag="JN691_002651"
     CDS             complement(5490..6446)
                     /gene="fepB"
                     /locus_tag="JN691_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY7244064.1"
                     /translation="MRLAPLHRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGAATPNNRVADDQGFLRQWSKVAKERKLQQLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            6737..7912
                     /gene="entC"
                     /locus_tag="JN691_002652"
                     /pseudo
     CDS             6737..7912
                     /gene="entC"
                     /locus_tag="JN691_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            7922..9532
                     /gene="entE"
                     /locus_tag="JN691_002653"
     CDS             7922..9532
                     /gene="entE"
                     /locus_tag="JN691_002653"
                     /EC_number="2.7.7.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase"
                     /protein_id="HAY7244065.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGHLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            9546..10403
                     /gene="entB"
                     /locus_tag="JN691_002654"
     CDS             9546..10403
                     /gene="entB"
                     /locus_tag="JN691_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HAY7244066.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            10403..11149
                     /gene="dhbA"
                     /locus_tag="JN691_002655"
     CDS             10403..11149
                     /gene="dhbA"
                     /locus_tag="JN691_002655"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="HAY7244067.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            11152..11565
                     /gene="entH"
                     /locus_tag="JN691_002656"
     CDS             11152..11565
                     /gene="entH"
                     /locus_tag="JN691_002656"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HAY7244068.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            11746..13851
                     /gene="cstA"
                     /locus_tag="JN691_002657"
                     /pseudo
     CDS             11746..13851
                     /gene="cstA"
                     /locus_tag="JN691_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
     gene            14033..14230
                     /locus_tag="JN691_002658"
     CDS             14033..14230
                     /locus_tag="JN691_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY7244069.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(14240..15328)
                     /gene="hcxA"
                     /locus_tag="JN691_002659"
     CDS             complement(14240..15328)
                     /gene="hcxA"
                     /locus_tag="JN691_002659"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HAY7244070.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            15437..16597
                     /gene="ybdL"
                     /locus_tag="JN691_002660"
     CDS             15437..16597
                     /gene="ybdL"
                     /locus_tag="JN691_002660"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HAY7244071.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            complement(16598..>16935)
                     /locus_tag="JN691_002661"
     CDS             complement(16598..>16935)
                     /locus_tag="JN691_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244072.1"
                     /translation="MEIVDGFHHHEIGKGSSSLKLRLKGYLPVTCLEDTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
BASE COUNT         3741 a         4420 c         4843 g         3931 t
ORIGIN      
        1 tacagaaaat aattatgggt tttttatttg tttgatttat aggtttgatg aatatttctc
       61 ttaaatagag tgaatgttgc attcagtgga aggaatttac aaacttcagc ttcggataat
      121 tctcaccaac tggtaaagtg agcgccgtgg agattttccc ctattggact cattttcagg
      181 ttatacccca tgtcatcatt gaatattaaa cagggaagtg acgctcattt ttctgagtat
      241 cctctggcgt cgcccagtaa taatgaaatt gatttactta atctaatcga agttttatgg
      301 cgggccaaaa aaacggtcat ggcggtcgtt tttgcatttg cctgcgcagg tttactgatc
      361 tctttcatcc tgccgcaaaa atggaccagc tcagccgtta ttactcccgc cgaagccatt
      421 cagtggcagg acctggagaa aacctttacc aaactccgcg ttcttgatct cgatgttaat
      481 atcgaccgtg gcggtgcatt taacctgttt atcaagaagt ttcagtctgt tagcttgctg
      541 gaagagtacc tgcgttcatc accttatgtg atggaccaat taaaagaggc gaaaatcgat
      601 gaactggatt tgcatcgcgc tattgttgca ttgagcgaaa aaatgaaagc ggttgatgac
      661 aatgccagta agaaaaaaga tgaaccgtca ctgtatacct cctggacgct aagttttacc
      721 gcgccaacca gtaaagaggc gcagaccgtt ttgagcgggt atatcgatta tatctctgcg
      781 ttggtggtga aagagtcgat agaaaacgtc cgtaataaac tggagatcaa aacccagttt
      841 gaaaaagaaa aactggctca ggatcgcatt aaaacgaaaa atcaacttga tgcaaacatt
      901 cagcgcctca attattcact cgacattgcc aacgcggcag gaattaaaaa gcccgtatac
      961 agtaatggtc aggccgttaa agatgacccc gatttctcta tttctctcgg cgcagacggt
     1021 attgaacgca aactggaaat tgaaaaagcg gttactgacg ttgcggaact gaacggtgaa
     1081 ttacgtaatc ggcagtatct tgtcgagcaa ttaacaaaag caaatgtcaa cgatgtgaat
     1141 tttacgccgt ttaaatatca gttaagcccg tcattgccag tgaaaaaaga cggtccgggt
     1201 aaggcgatta ttgtgatcct ttccgcgctg atcggcggga tggtggcttg tggtagcgtg
     1261 ttattgcgct atgcgatggc atccagaaaa caggatgcca tgatggcaga ccacttagtt
     1321 taactatttg cggttgaagg tgccgttcgt ccgagcggca ccacaagcgg cgttccggct
     1381 accggatcgt caatgatcat gcatcgcaga ccataaatgc gctcaatcag ttcagcagtg
     1441 acaatctcct tcggcgctcc ctgagcaaca attttccctt cccgcaatgc aatcaaatgg
     1501 ctggcgtaac gacaggcctg attaagatcg tgcagcaccg ccgccagggt atagcctttc
     1561 tcgcggttca gttcgcttaa cagttccagt agatcaatct gatgactgat atccagccag
     1621 gtcgtgggtt cgtcgagcag cataattgcc gtttcctggg ccagcaccat cgcgatccac
     1681 gctcgctggc gttgtccgcc agaaagggta tccacgcttt gatctgccag atgagttatt
     1741 cccgtggcct gcatcgcttt cgttaccgct tcttcatcct ctttgcgcca gcgggtaaac
     1801 agcggttgat gcggataacg tccacgcgcc accagctcct gcacggtgat atcgcccggc
     1861 gtggtagcat tttgcgccaa cagaccaatc cggcgtgcaa cttctttact ggcgtaatgt
     1921 tgaatgtgct cgccatccag ccagacatgc ccgtgagcag gcgtcatcag gcggcttaag
     1981 gtacgcagta acgtggattt accacagcca tttggtccga taattgccgt gaagtgacca
     2041 tcaggaattt ctacagtcag attttccgca acggtatatt tgccatatcc cagggttaac
     2101 tgttcgccgc gcaaacgggc tactgattcg gtcatttttt gcgagactcc tgaattaaca
     2161 agacgataag gtaaataccg ccgaggctga cggtaacgac gccaaccgga agttgatacg
     2221 gcataaacag ttgttgggcg cagagatcgg ccgccagcag taacagcgct ccgcacagcg
     2281 ccgcctgggt cagcccccag cgagcggtgc cgctaatgcg tcgggcaatg tgcggcgcga
     2341 ctaaagcaat aaaggaaatc ggcccggcaa gcgccgttgc cgcagcggta agcaccactg
     2401 caaccagcat cattaacaga cgcgaacgct cgacgctgac gcccagcgcg caggcggtat
     2461 catcgcccat ctccagcaag cgcattcgcc gcaccagcaa ggtggcggca atgagcatca
     2521 atataatgat gggtgcggaa ggcgaggttt ttgcccacgt caggccgttg agcgatccgg
     2581 cattccacaa acctgctgtt agcgccgttt ctaaagacgc tttcagcaac agccaggtat
     2641 taaaggccac cagcatggcg cgaacgccga taccgataat aatcaaccga aaggtgtcga
     2701 taccgttacg ccaggcgagc agccagacca acagcgaagt gataatgccg cccaccattg
     2761 ctgccagcgc gatagccgtc agatcctgac caaacagcac catcgccacc agcacgccgc
     2821 tccacgcccc ggtgttaaag cccattacgt cagggctgcc gagcgggtta cgcatcagcg
     2881 actgaaaaat cgcgccgctg acgcccagcg ccgcgccaat caacagcgcc atcagcacgc
     2941 gtggtaaacg ccattcggtg accaccatcg tcatactgcg cggcgcatcg cccatcaacg
     3001 cggcgaatac ctgcgagatt tccagcgtga cggcaccgct gcgtaatccc cagatacctg
     3061 caaccacaca ggcggaaatc agcagcaaac aggtgatgag taatcggcga gagacgtaaa
     3121 tcatgcacca cctcgcgttt tacgtcgcac gaggaagatc agcaccggtg caccaataaa
     3181 cgcactgacc acagaaacgc gcagttcgcc gggcacaatc acccgcccga tgatatcggc
     3241 aaacagcagc agggcagggg tagcaagtag cgtgacgggc agcgaccagc gatgatcggc
     3301 acctaccagc cagcgcgcca tgtgcggcat catcaggccg ataaaggcaa tcgggccaac
     3361 taccgccgtc gcactaccac aaagcacggt aatcgccagc agaccaatca actgtgtgcg
     3421 cgccacgcga ctgcccagcg ccgtcgcggt gtcactgccg aggctcaaac tgttcagcgc
     3481 gcgactcagt aatagcgcag ttgctccggc gatcagaacc gggatcagca ccacttttaa
     3541 ggtatgtaga ttgcgaatat ccagcgaacc ggcttgccag aaacgcagtt gatcgtagac
     3601 gtcaggatta agcagggcga tgccgctggt cagtccttcc agcaccgccg ccagcgccac
     3661 gcccgccagg gttaaacgca ccggacttaa ctgccctcca ccctgactgc cggtaaaggc
     3721 aacaatcaat gaggccacca gcgccccggc gaaggccatc gccagttgtt cctgtgcgga
     3781 tgagtaacca aacagcgccg cacccagcac aatggcaaag ctggctccgg cgttcacgcc
     3841 aagcaagccg gggtcggcaa gtgggtttcg ggtgagggtt tgcattaacg ccccggcaag
     3901 gccaagcgcg ccgcctgcca gtaaaccggc aagggtacgc ggcagccgcg cgtcgagcac
     3961 gatggtgcag tcggcgctct ggcaggtgcc ggagaaggcc tccagcacta cggaagcggg
     4021 gagtgatttt gccccaatga gcaggcttaa tgccgtcgcg ataatcagta ataacagcaa
     4081 tccgggcacg gcaatggcgc gtgtcacggc aacagaacca gacatacgaa cttccatgat
     4141 aatgaaatta attatcgtta tcgatcttat ttggatatgt tagcatgtgc agcctaagaa
     4201 taggtattta aaatatttga tggcaaggca ttgtaatgaa taaacaatcc tggctgctta
     4261 acctcagcct gttgaaaacg cacccggcgt ttcgcgcagt attcctcgct cgttttatct
     4321 caattgtgtc tctgggtttg ctcggcgtcg cggtgccggt gcagatccag atgatgaccc
     4381 attctacctg gcaggtgggg ctttcggtga cgctgaccgg cggcgcgatg tttgttggcc
     4441 tgatggttgg cggtgtgctg gcggatcgct atgaacgcaa aaaagtgatt ttgctggcgc
     4501 gcggcacctg tggcattggc ttcattggac tgtgccttaa tgcactgctg ccggagccgt
     4561 cattgctggc aatctattta cttggtttat gggatggttt tttcgcatca cttggtgtta
     4621 cggcgctact ggcggcaaca tctgcactgg tagggcgtga aaacttaatg caggccgggg
     4681 cgatcaccat gttgaccgtg cgcctggggt cggtgatttc gcccatgatt ggcggtttat
     4741 tgctggcgac cggcggcgta gcctggaact acgggctggc ggcggcgggc acgtttatta
     4801 ccttgttacc gttattaagc cttccggcgt tgccaccgcc accgcagccg cgtgagcatc
     4861 cgttgaaatc attactggca ggatttcgtt ttctgcttgc cagcccgctg gtgggcggga
     4921 ttgcgctgct gggtggttta ttgacgatgg cgagcgcggt gctggtactg tatccggcgc
     4981 tggctgacaa ctggcagatg tcagcggcac agattggttt tctctacgcg gcgatcccgc
     5041 tcggcgcggc tattggtgcg ttaaccagcg ggaagctggc acatagtgcg cgaccagggt
     5101 tattgatgct gctctccacg ctgggatcgt tcctcgccat tggtctgttt ggcctgatgc
     5161 cgatgtggat tttaggcgtg gtttgtctgg cgctgttcgg ctggctgagc gcggttagtt
     5221 cgttgctgca atatacaatg ctgcaaacgc aaaccccgga agcgatgtta gggcggatta
     5281 acggtttgtg gacggcgcag aacgtgacgg gcgatgccat aggcgcggcg ctgctgggtg
     5341 gtttgggcgc gatgatgaca ccggttgctt ccgcaagcgc gagcggtttt ggtttgttga
     5401 ttatcggcgt gttgttattg ctggtgctgg tggagttgcg acgttttcgc cagacgccgc
     5461 cgcaggtgac agcgtccgac agttaatgct taaaacagcg ccttaagccg ttccagcact
     5521 tgcatggcgc tgtagtaatc caggcggaac gtctcggttc ccagcgcata aacctgcttg
     5581 ttttgtaccg caggcaggtg cgcgagcagt ggattggcat aaatcgcatc ggcatctttc
     5641 tgatcaccag cgaacaggaa tagcgattca ccgtttaacc ccgcagccag attttcccca
     5701 ccaagctgaa tgatgtcatg gcgtttgccc tggctttggc tggcgtttaa ccctgcgggc
     5761 aacttcgcca gcgtaaagcc gagttgttcc agcatctgcc cttgtgctga ttccggcgtc
     5821 cagagattgg cactgtgtgc agcggcggta tagacaatgg cagtgaccgg ctgcggcggt
     5881 aatttgattt gctctttcgc cgctgccagt tgcttatcaa actgtgcaat ccgctctgcc
     5941 gcttgtttct catgcccggt aatttcgcca agctgcgtta acagcgactg ccagcttttg
     6001 tcgtcgtaat tgatgattaa tgtcggggcg atggtggaaa gctgatcata cagcgccagc
     6061 gccgaatccc cgccggttgc gctaattaaa atcagatccg gcatttgcgc ggcaacggct
     6121 tcggcgctcg gttcgccgat atagagctgt tgcagtttgc gttctttcgc caccttgctc
     6181 cactggcgta aaaagccctg gtcatctgcg acgcggttat tcggcgtggc cgcgccgctg
     6241 gcgatcaccg gagcatcaat cgccagcagt gatccggtca gggtgacgct ggtggaaaca
     6301 atacgctgcg gctggctttc cagggtatga gtgccccggc tgtcagtaat ctgacgcggc
     6361 cagtcggcgg cctgaactac ggctattcct gaaagcaaaa gtcctgttaa tagaagggcg
     6421 ttgcggtgga gcggggcgag tctcacaagt cagcttcctg ttattaataa ggttaagggc
     6481 gtaatgacaa attcgacaaa gcgcacaatc cgtccctttg gggagagggg gcaaaacggc
     6541 gcaggacatc acattgcgct tatgcgaatt catcaataat gcttctcatt ttcattgtaa
     6601 ccacaaccag atgcaacccc gagttgcaga ttgcgttacc tcaagagttg acatagtgcg
     6661 cgtttgcttt taggttagcg gccgaaaata taaatgataa tcattattaa agcctttatc
     6721 atttgtgggg gatgatatgg atacgtcact ggctgaggaa gtacagcaga ccatggcaac
     6781 acttgcgccc aatcgctttt tctttatgtc gccgtaccgc agttttacga cgtcaggatg
     6841 tttcgcccgt ttcgatgaac cggctgtgaa cggggattcg cccgacagtc ccttccagca
     6901 aaaactcgcc gcgctgtttg ccgatgccaa agcgcagggc atcaaaaatc cggtgatggt
     6961 cggggcgatt cccttcgatc cacgtcagcc ttcgtcgctg tatattcccg aatcctagca
     7021 gtcgttctcc cgtcaggaaa aacagacctc agcccgctgt tttacccgca gccagtcgct
     7081 gaacgtggtg gaacgccagg caattcctga acaaaccacg tttgaacaga tgggtgcccg
     7141 cgccgccgca cttaccgcca cgccgcaggt cgacaaagtg gtgttgtcac gattgattga
     7201 tatcaccact gacgccgcca ttgatagtgg cgtattgctg gaacggttga ttgcgcaaaa
     7261 cccggttagt tacaacttcc atgtcccgct ggctgatggt ggcgtcctgc tgggggccag
     7321 cccggaactg ctgctacgta aagacggcga gcgttttagc tccattccgt tagccggttc
     7381 tgcgcgtcgt cagccggatg aagtgctcga tcgcgaagcg ggtaatcgtc tgctggcgtc
     7441 agaaaaagat cgccatgaac atgaactggt gactcaggcg atgaaagagg tactgcgcga
     7501 acgcagtagt gagttacacg ttccctcctc tccacaattg attaccacgc cgacgctgtg
     7561 gcatctcgca actccctttg aaggtaaagc gaattcgcaa gaaaacgcac tgactctggc
     7621 ctgtctgctg catccgaccc ccgcgctgag cggtttcccg catcaggccg cgacccaggt
     7681 tattgctgaa ctggagccgt tcgaccgcga actgtttggc ggcattgtgg gttggtgtga
     7741 cagcgaaggt aacggcgaat gggtggtgac catccgctgc gcgaagcagc gggaaaatca
     7801 ggtgcgtctg tttgccggag cggggattgt gcctgcgtcg tcaccgttgg gtgagtggcg
     7861 cgaaacaggc gtcaaacttt ctaccatgtt gaacgttttt ggattgcatt aaggagcgag
     7921 gatgagcatt ccattcaccc gctggccgga agagtttgcc cgtcgctatc gggaaaaagg
     7981 ctactggcag gatttgccac tgaccgacat tctgactcgc cacgctgcga gtgacagcat
     8041 cgcggttatc gacggcgagc ggcagttgag ttatcgggag ctgaatcagg cggcggataa
     8101 cctcgcctgt agtttgcgcc gtcagggcat taaacctggt gaaaccgcgc tggtacaact
     8161 gggtaacgtc gctgaacttt acattacctt tttcgcgctg ctgaaactgg gcgtcgcgcc
     8221 ggtgctggcg ctgttcagcc atcagcgtag tgaactgaac gcctacgcca gccagattga
     8281 accggcgtta ctgattgccg atcgccagca tgcgctattt agcggggatg atttcctcaa
     8341 cacatttgtt gcagagcatt cttccattcg cgtggtgcaa ctgcacaacg acagcggtga
     8401 gcataacttg caggatgcga ttaaccatcc ggcagagggt tttactgcca cgccatcacc
     8461 tgctgatgaa gtggcctatt tccagctttc cggcggcacc accggcacac cgaaactgat
     8521 cccgcgcact cataacgact attactacag cgtgcgtcgt agcgtcgaga tttgtcagtt
     8581 cacacaacag acgcgctacc tgtgcgcgat cccggcggct cataactacg ctatgagttc
     8641 gccaggatcg ctgggcgtct ttcttgccgg aggaacggtt gttctggcgg ccgatcccag
     8701 cgccacgctt tgcttcccgt tgattgcaaa acatcaggtt aacgttaccg cgctggtgcc
     8761 gccggcagtc agcctgtggt tgcaggcgct ggctgaaggc gaaagccggg cgcagcttgc
     8821 ctcgctgaaa ctgttacagg tcggcggcgc acgtctttct gccacgcttg cagcgcgtat
     8881 tcccgctgag attggctgcc agttgcagca ggtgtttggc atggcggaag ggctggtgaa
     8941 ctacacccgt cttgatgata gcgcggagaa aattatccat acccagggtt acccaatgtg
     9001 tccggacgat gaagtgtggg ttgccgatgc cgaaggaaat ccactgccgc aaggggaagt
     9061 tggacacctg atgacgcgcg ggccgtacac cttccgtggc tattacaaaa gtccgcagca
     9121 caatgccagt gcctttgatg ccaacggttt ttactgttcc ggcgatctga tctctattga
     9181 tccagagggt tacatcaccg tgcaggggcg cgagaaagat cagatcaacc gtggcggcga
     9241 gaagatcgct gccgaagata tcgaaaacct gctgctgcgc catccggcag tgatctatgc
     9301 cgcactggtg agcatggaag atgagctgat gggcgaaaaa agctgtgctt atctggtggt
     9361 aaaagagccg ctgcgcgcgg tgcaggtgcg tcgtttcctg cgtgaacagg gtattgccga
     9421 atttaaatta ccggatcgcg tggagtgtgt ggattcactt ccgctgacgg cggtcgggaa
     9481 agtcgataaa aaacaattac gtcagtggct ggcgtcacgc gcatcagcct gaaggagaga
     9541 acacgatggc tattccaaaa ttacaggctt acgcactgcc ggagtctcac gatattccgc
     9601 agaataaagt tgactgggcc tttgaaccgc aacgtgccgc gttgttaatc catgatatgc
     9661 aggactattt tgtcagcttc tggggcgaga actgcccgat gatggagcag gtgatcgcga
     9721 atattgctgc gctgcgcgac tactgcaaac agcacaatat cccggtttat tacaccgccc
     9781 agccgaaaga gcagagcgat gaagatcggg cgctgttgaa tgatatgtgg gggccgggcc
     9841 tgacccgctc gccggaacag caaaaggtgg tggatcgcct gacgccagat gccgacgaca
     9901 cggtgctggt gaagtggcgc tacagcgcgt ttcatcgttc tccgctggag caaatgctca
     9961 aagagagtgg acgtaaccag ctgattatta caggggtata tgcccacatt ggctgtatga
    10021 ccaccgcaac cgacgcattt atgcgcgata ttaaaccgtt tatggtggcg gatgcgctgg
    10081 ctgatttcag ccgtgacgag catttgatgt cgctgaaata tgtggccgga cgttctggcc
    10141 gggtggtgat gaccgaagaa ttactgccag cacctatccc cgccagcaaa gcggcgctgc
    10201 gtgaggtgat tctgccgttg ctggacgagt ccgatgaacc gttcgatgac gacaacctga
    10261 tcgactacgg tctggattcg gtgcgcatga tggcgctggc ggcgcgctgg cgcaaagtgc
    10321 atggtgatat cgactttgtc atgctggcga aaaacccgac catcgacgcc tggtggaagc
    10381 tactctcccg cgaggtgaaa taatggattt cagcggtaaa aatgtctggg taaccggcgc
    10441 aggtaaaggt atcggctacg ccacggcgct ggcgtttgtt gaggcgggag cgaaagttac
    10501 aggttttgat caagcgttca ctcaggagca atatcccttt gcgaccgaag taatggatgt
    10561 tgccgacgcc gggcaggtgg cgcaggtatg tcagcgactg ttagctgaaa cggagcgact
    10621 ggacgtgctg atcaatgcgg cgggaatttt gcgcatgggc gcgaccgatc agctcagtaa
    10681 agaggactgg cagcagactt ttgcggttaa cgtcggcggt gcgtttaacc tgttccagca
    10741 aaccatgaac cagtttcgcc gtcagcgggg cggggcgatt gtcactgtgg cgtccgacgc
    10801 cgcacacacg ccgcgtattg gtatgagtgc ttatggcgca tcgaaagcgg cgctgaaaag
    10861 cctggcgttg agcgtcgggc tggaactggc gggtagcggc gtgcgctgta atgtggtttc
    10921 gcctggctcc accgacaccg atatgcaacg cacgctgtgg gtgagcgatg acgccgaaga
    10981 acagcgtatt cgcggctttg gcgagcagtt taaactcggc attccgctgg ggaaaatcgc
    11041 ccgtccacaa gagatcgcca acacgatttt gttcctcgcc tctgacctcg ccagccatat
    11101 caccctacag gatattgtgg tcgatggcgg ctcaacgctg ggggcataag catgatctgg
    11161 aaacgccatt taacgctcga cgaactgaac gccaccagcg ataacacaat ggtggcgcat
    11221 ctgggaattg tgtatacccg tctgggagat gatgtgctgg aagccgaaat gccggttgat
    11281 acccgtactc atcagccgtt tggcctgctg catggcggcg cgtcggcggc gctggctgaa
    11341 acgctgggat cgatggccgg atttatgatg acccgtgacg ggcagtgtgt ggtgggcacg
    11401 gaacttaacg ccacccatca tcgcccggtg tctgaaggca aggtacgcgg cgtctgccag
    11461 ccgctgcatc ttgggcggca aaatcagagc tgggaaatcg tcgttttcga tgaacagggg
    11521 cggcgttgct gcacttgtcg gctgggtacg gcagttttgg gatgagcccg acagaattaa
    11581 atgagattgc aggaaaactc ggttaacgga gtgatcgagt taacattgtt aagttaaata
    11641 ttggtttcaa ctccgattta catggttacc gtgttgttaa atcgcacaaa gatgttatag
    11701 aaacaaaatg taacatctct atggacacgc acacggataa caactatgaa caaatcaggg
    11761 aaatacctcg tctggacagt gctctctgta atgggagcat ttgctctggg atacattgct
    11821 ttaaatcgtg gggaacagat caacgtgctg tggattgtgg tggcgtcggt ctgtatctat
    11881 ctgatcgctt accgttttta tgggctgtat atcgccaaaa atgtgctggc ggttgacccg
    11941 acgcgtatga cgccagcggt gcgccataac gacgggctgg actatgtgcc gacggacaag
    12001 aaagtgctgt tcggtcacca ttttgcggcc attgccggag cagggccgct ggtggtgccg
    12061 gtgctggcag cgcaaatggg ctacctgccg gggatgatct ggctgctcgc cggggtggtt
    12121 ctcgccggtg cggtgcagga tttcatggtg ctgtttgttt ctacgcgccg tgacggtcgc
    12181 tcgctgggtg agctggtcaa agaagagatg gggccaactg ccggggtgat tgcgctggta
    12241 gcctgcttta tgatcatggt cattatcctt gcagtactgg cgatgatcgt ggtgaaagcc
    12301 ctgactcata gcccgtgggg aacgtatacc gttgcgttca ccattccgct ggcgattttt
    12361 atgggaatct acctgcgcta tctgcgtccg ggccgcattg gcgaagtgtc agtgattggc
    12421 ctggtgttcc tgattttcgc cattatctct ggcggctggg tggcagaaag tccgacctgg
    12481 gcaccgtact ttgactttac cggcgtgcag ctgacctgga tgctggtggg ctacggtttt
    12541 gtggcggcgg tgctgccggt gtggttgctg ctggccccgc gtgactatct ctcgaccttc
    12601 ctgaaaatcg ggactatcgt cggtctggcg gtaggcattt tgattatgcg cccgacgctg
    12661 accatgcctg cgctgaccaa atttgtcgat ggcactggcc cggtatggac cggtaacctg
    12721 ttcccgttcc tgtttatcac catcgcctgt ggtgcggtgt ctggcttcca tgcgctgatc
    12781 tcttccggca ccacgccgaa gatgctggcg aacgaagggc aggcgtgctt tatcggctac
    12841 ggtgggatgt tgatggaatc cttcgtggcg attatggcgc tggtttctgc ctgtatcatc
    12901 gatccgggcg tgtattttgc catgaacagc ccaatggcgg tgctggctcc ggcagggacg
    12961 gcggatgtag tcgcttctgc cgcgcagatg gtgagtagct agggctttag cattacacca
    13021 gatacgctca accagattgc cagcgaagtg ggtgaacagt cgatcatttc ccgtgcgggc
    13081 ggtgcgccga cgttggcggt ggggatggct tacatcctgc acggtgcgct gggtggtatg
    13141 atggatgtgg cgttctggta tcacttcgcc attctgtttg aagctctgtt tattctgacg
    13201 gcggtagatg cgggtacgcg tgctgcgcgc tttatgttgc aggatctgct gggcgtggtg
    13261 tctcctggcc tgaagcggac cgattcactg cctgctaacc tgctggcaac ggcgctgtgc
    13321 gtactggcgt ggggctactt cctccatcag ggcgtggtcg atccgctggg cggtattaac
    13381 actctgtggc cgctgtttgg tattgccaac cagatgctgg cagggatggc gctgatgctc
    13441 tgtgccgtgg tgttgttcaa gatgaaacgt caacgttacg cctgggtggc gctggtacca
    13501 acggcctggc tgctgatttg taccctgacc gctgactggc agaaagcgtt tagcccggat
    13561 gcgaaagtcg gcttcctggc cattgctaac aagttccagg caatgatcga cagcggcaat
    13621 attccgtcgc agtatactga gtcacagctg gcgcaactgg tgttcaacaa ccgtctggat
    13681 gccgggttaa ccatcttctt tatggtggtc gtggtggttc tggcactgtt ctcgattaag
    13741 acggcacttg cggcattgaa agatccgaag ccaacggcga aagaaacgcc gtatgagccg
    13801 atgccggaaa atgtcgagga gatcgtagcg caggcgaaag gcgcgcacta aagtcagagt
    13861 gaggggggat gttggcgaat gttggcatag tgccgagggt tccccctcac cctaaccctc
    13921 tccccgatgg ggcgagggga ctgaccgagt gcgttgatag catttgtagg ccggataaag
    13981 cgttcacgcc gcatccggca ctctttcagc aacatggtta gcggaggcca agatgtttga
    14041 ttcactggca aaagccggaa aatatttagg tcaggcggcg aagctgatga ttggtatgcc
    14101 tgattacgac aactatgtcg aacatatgcg ggttaatcat cccgatcaaa cgccgatgac
    14161 ctacgaggag tttttccgtg agcggcagga cgcgcgctac ggtggaaaag gcggcgcgcg
    14221 ctgctgctaa atttgcacgt caggctttaa acgattccac tttttcgaac gctgcacgca
    14281 acgtatctgg tgtcagcgtg actggcaggt aatgaatgga ctccaccggg cgcaaggtgt
    14341 gggcaatcac tttgtcgatc tccgcctgat tattgatatc cacctccagc tccgccagcg
    14401 tagtcggcag atgaaaacgc tgatacgccc cggttaattg cgccagcaca tcatcctgac
    14461 ccagcaaggc gctttgcacc agaattccgt aagcgacttt ggtgccgtga agaaacttct
    14521 cggtttgcgg caggacagtc agaccgttat gcacggcatg agccgccgcc acacgcgtaa
    14581 aacgatcgcc cagaccaccg accatcccac caccagcaat aatagcatcc accacatcgc
    14641 aaaatgattg cgttaactgc tgattttgct gatcggcaag tgcctgttcg ctactgttta
    14701 acaagacgtc gcgaatggcc tgcgcattat tgatccccag ccgcacggtt agcggcaacg
    14761 tttctggttg cggagccagc accaccgctt cataccattt cgccagcgtg tcaccgatcc
    14821 ccgccagcag atattgttgc ggtgcattga ggataatctc cggttccacc agcaccataa
    14881 aattggcgtc gtcgaaaatc tcataatgca gcgcctgtcc ggcatcgtta taccagacgg
    14941 agagcggtgt ccaggctgcg caggtggcgg cgatcgtcgg agcggcaaca aatggcagac
    15001 cgagacggcg ggcgagggct ttcgcggtgt cgagcagtgc gccgccgccg acgccaatca
    15061 ccacgctgcg atcgtcaccg gactcagccg ccagttgctg tacatcgctt tcgctgcaat
    15121 gaccacgaaa caaaatatgc tttgcccctg gaagtccaaa cgctggcgga agtttggttt
    15181 gcgccgcagc aatggcgcgt ttgccgtaga tccacaccgc gcgagaaagt tgttcatcag
    15241 tgaaaaaatt gtgcaggtga ttgaaacttc ctggatgtga aaagtagtta gccgggccga
    15301 cgaccacgcg gataggattg tgaggcatgt tgtgttgtcc ttattgtttt atttagacat
    15361 ctaaacgtct tgattgccaa atactagcat cgtgttatag tgccttcaac acgcaacttc
    15421 gtcaggtaca ataaaaatga caaataaccc tctgattcca caaagcaaac ttccacaact
    15481 tggcaccact attttcaccc agatgagcgc gctggcgcag caacaccagg cgattaacct
    15541 gtcgcaaggc tttcctgatt ttgatggtcc gcgctattta caggagcggc tggcgtacca
    15601 cgttgcccag ggggcaaacc aatacgcgcc catgaccggc gtgcaggcct tgcgcgaggc
    15661 gattgctcag aaaacggaac gtttgtatgg ctatcaacca gatgccgata gcgatatcac
    15721 cgtaacggca ggggcgacgg aagcgttata cgcggcgatt accgcactgg tgcgcaatgg
    15781 cgatgaagtg atttgttttg atcccagcta tgacagttac gcccccgcca tcgcgctttc
    15841 tgggggaata gtgaagcgta tggcactgca accaccgcat tttcgcgttg actggcagga
    15901 atttgccgca ttattaagcg agcgcaccag actggtgatc ctcaacactc cgcataaccc
    15961 cagtgcaact gtctggcagc aggctgattt cgccgctttg tggcaggcga tcgccgggca
    16021 cgagattttt gtcattagcg atgaagtcta cgagcacatc aacttttcac aacagggcca
    16081 tgccagtgtg ctggcgcatc cgcagctgcg tgagcgggca gtggcggttt cttcatttgg
    16141 caagacctat catatgaccg gctggaaagt gggttattgt gttgcgccag cgcccatcag
    16201 cgccgaaatt cgcaaggtac atcagtatct gaccttttcg gtgaataccc cggcacagct
    16261 ggcgcttgct gatatgctac gtgcagaacc tgagcattat cttgcgttac cggactttta
    16321 tcgccagaag cgcgatattc tggtgaatgc tttaaatgaa agccggctgg agattttacc
    16381 gtgtgaaggt acatactttt tgctggtgga ttacagcgcg gtttctaccc tggatgatgt
    16441 tgagttttgc cagtggctga cgcaggagca cggcgtagcg gcgattccgc tgtcggtgtt
    16501 ttgcgccgat cccttcccac ataaactgat tcgtctctgt tttgccaaga aggaatcgac
    16561 gttgctggca gcagctgaac gcctgcgcca gctttagcta tttaaccgtc caggcgcggg
    16621 aatattgacg gtcggcaaac aactcttgca ggccgttaat ttgcttcagg cgcagcactt
    16681 cgtcgctgtc cattcccagc tctttgccaa ttttattatc gtcccatccc aactggctaa
    16741 gttcacggac gatttccgac atggcggtga tttgatggcg tccacgagcg cgattatgac
    16801 gaatagtcgc ggcgatgcgt tgattacgcg tatcttccag acaggtcacc ggcaaataac
    16861 cttttaagcg taattttaat gatgatgagc ctttaccaat ttcatggtgg tgaaaaccat
    16921 ccacgatttc catgg
//
LOCUS       DADLSA010000084        16899 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.084, whole genome shotgun sequence.
ACCESSION   DADLSA010000084 DADLSA010000000
VERSION     DADLSA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.084"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="JN691_002662"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="JN691_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7244073.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="JN691_002663"
     CDS             2219..2767
                     /locus_tag="JN691_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAY7244074.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="JN691_002664"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="JN691_002664"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAY7244075.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /locus_tag="JN691_002665"
     CDS             complement(3664..4854)
                     /locus_tag="JN691_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAY7244076.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JN691_002666"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JN691_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAY7244077.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="JN691_002667"
     CDS             complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="JN691_002667"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAY7244078.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="JN691_002668"
     CDS             complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="JN691_002668"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAY7244079.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9767..12379
                     /gene="pepN"
                     /locus_tag="JN691_002669"
     CDS             9767..12379
                     /gene="pepN"
                     /locus_tag="JN691_002669"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAY7244080.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12586..13353)
                     /gene="ssuB"
                     /locus_tag="JN691_002670"
     CDS             complement(12586..13353)
                     /gene="ssuB"
                     /locus_tag="JN691_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY7244081.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGPDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13350..14141)
                     /gene="ssuC"
                     /locus_tag="JN691_002671"
     CDS             complement(13350..14141)
                     /gene="ssuC"
                     /locus_tag="JN691_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HAY7244082.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
     gene            complement(14153..15298)
                     /gene="ssuD"
                     /locus_tag="JN691_002672"
     CDS             complement(14153..15298)
                     /gene="ssuD"
                     /locus_tag="JN691_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HAY7244083.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(15295..16254)
                     /locus_tag="JN691_002673"
     CDS             complement(15295..16254)
                     /locus_tag="JN691_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7244084.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRTGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16247..16822)
                     /gene="ssuE"
                     /locus_tag="JN691_002674"
     CDS             complement(16247..16822)
                     /gene="ssuE"
                     /locus_tag="JN691_002674"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAY7244085.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVL
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4219 a         4373 c         4327 g         3980 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgtacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgcgatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cgctcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagccca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcag ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgat
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaat atgcaaccaa aagtaaaaat taaggagctt tgtgaacacc
     6361 gtcatatttc catagaaacg tgatgatatt tacagcaatt tttatctatt tatatgattt
     6421 acttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
     6481 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
     6541 gctaagaaaa aactaatccg caataagaat gcgggttatt ttttgtgcgt aaatgttgca
     6601 atgtaatatt ccgtaacagg atgatcgtta tggcgggggt gcaaatagat tgcttgactt
     6661 aacaaacaga taacttgaca gaaaggataa aaaagagcca gcaggcgctg gctctttgag
     6721 gaagaaaatc agaagctggc gttgcgcgga gtacgtggga acggaatcac atcacgcacg
     6781 ttttgcacgc cagttacgta agcaatcagg cgttcaaaac caagaccgaa acctgaatgc
     6841 ggaacagtac cgtagcgacg cagatcgcga taccaccagt aatcttcttt attcaggccc
     6901 atttccagca tacgctcgtc cagaacgtcc agacgttctt cacgctggga gccaccaatg
     6961 atctcaccga tgcccggagc cagaacgtcc atagccgcaa cggttttacc gtcttcgttg
     7021 aggcgcatat agaacgcttt aatatctttc ggatagtttt taaccactac cggtgcttta
     7081 aagtgttctt ccgccagata acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc
     7141 gggttttcaa acttcctgcc gcagttttcg agaatggtca ctgcgtcggt gtaatccacc
     7201 tgcgcgaaat cggcttcaat gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt
     7261 tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc
     7321 agcatggctt cagccagacc cgcgatatcg ttcaggttag caaacgccac ttccggctcc
     7381 agcatccaga attccgccag gtgacggctg gtgttggagt tttcagcacg gaaagtcggg
     7441 ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat
     7501 acggtcagga aagactcttt accaaagaag tctttgtcga aatccacttt gccctgatcg
     7561 ttacgcggca ggttttccag atccagcgta gaaacgcgga acatttcgcc tgcaccttcg
     7621 gtatcagatg cggtaatcag tggcgttgaa acccagaaga atccctgctc gttaaagaag
     7681 cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga cggcaccaat caggtttgtg
     7741 cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc
     7801 gggtaagtgt ctggatcttc aacccaacca gcaacttcaa ccttgctggc ctgaatttca
     7861 aattgttgcc cctggcccgg cgatgccacg actttacccg tcacaatgac cgagcagccg
     7921 gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag aattattgat gacagcctgt
     7981 acaggatcaa agcaggaacc gtcataaacg gcgaggaagg agatgccagc ttttgaatct
     8041 cggcgggtac gtacccatcc gcgtacggtg acttcgctgt caacggctac gcggccctgg
     8101 agtacgtcgg ctacaggcac aacgctcata atattctctc tgttaatagt cggaaaaaat
     8161 aaacacttgt ccacccgtaa atggggggga tacctatgtt acctggcatc tgcaatcaga
     8221 caagcagaat tcgcaaatgc agcgaaagat ttcggaatta aaggtaaaaa aagggagccg
     8281 attagctccc tgatgatatt aactggcttt tttaatatgc ggaaggtcga acgctttgcg
     8341 cagcgcccga acaaacgctt tatcatggca gatagttttg ccagggctgt cagaaagttt
     8401 cgccaccggt ttaccgttac actctaccaa cttaatcaca atattcaggg gttttacctg
     8461 ggggatatcg caggtcaggc gagtcccaat accaaaactt aattgcacgc gggaagagaa
     8521 gtggcggtat agctcaaccg ctttgcgtaa atccagattg tcagagaaaa ccagcgtttt
     8581 actctgtgga tcaattccca gcttttcata atgtgcaatg gctttttcac cccattcaac
     8641 cgggtcgcca gagtcatgac gcaggccctg ataccgacta gcgaactcga caccgaaatc
     8701 acgcaggaaa gcatccatag tgatgcagtc ggttaatgca atgccaagtt ggtcgggata
     8761 ctcttccagc caggcagcaa gtgcagctcg ctggctgttg gctagatccg ggctgatttg
     8821 ctgatgtgcc tggaaccatt cgtgtgcctg tgttcccatc ggcgtgaggg aaagccgacg
     8881 cgccagatcg tagttgctgg tgcccacgaa ccaggattcc tgttgcagac gcttaacgat
     8941 ggtttcttgt acttcgcgag aaaaacggcg acgggtgcca aaatccatca gatggaagcg
     9001 cgacatatca agaccggcgg ttaacgccga gaagtcgact aatttgcttt ccaacgtgtc
     9061 gagggcttgc gcaacgtcgg cctgcggtga gcgatagcga tgtaccattt cactgataac
     9121 cgccagcaaa ggaacttccc agaggatgac ttcacgccac ggaccgctta agcgaatatc
     9181 cagcttgcca ttatcgttgg acacggtgac ttgttccggg ttaaagcgga actcgcgtaa
     9241 ccagttaaga tagtcggcct taaagaaagg cagggcagag agccactgat attcatcatc
     9301 ctgcaggcgc aggtgctgca tcgcctgaat ctgttcacga atagcatcgg cataaatacc
     9361 cagcagatcg tcacctcggc aacgaaactc cgccgcgaca tgcacatcgt aatagtgatg
     9421 aaacacggct tgctgcatat gcaacttata agcatctgta tccagcaacg agtgcagaac
     9481 aggagaagcg aattgtgtca taggtgcgca gtagcgtcct cgtacaggag cgtttagtac
     9541 aataaacatc ttcaggaact gctggagtat accttgtttc gcaatttatt gaaccccgat
     9601 cacaccatat gccacctttc tggtcgatgg cattacgcgg tgtatgttat aaaaatgtag
     9661 caataaaggc gtttgtacct gaaaagatga agattctgca tagcgcgatt tacgcaacag
     9721 gaatagactg aacaccagac tctataaaag atgctaaagg ttatttatga ctcaacagcc
     9781 acaagccaaa taccgtcacg attatcgtgc gccggattac cagattactg atattgactt
     9841 gacctttgac ctcgacgcgc aaaagacggt cgttaccgcg gtcagccagg ctgtccgtca
     9901 tggtgcatca gatgctccgc ttcgtctcaa cggcgaagac ctcaaactgg tttctgttca
     9961 tattaatgat gagccgtgga ccgcctggaa agaagaagag ggcgcactgg tcatcagtaa
    10021 tttgccggag cgttttacgc ttaagatcat taatgaaata agcccggcgg cgaatacggc
    10081 gctggaaggg ctttatcagt caggcgatgc gctttgcacc cagtgtgaag ccgaaggttt
    10141 ccgccatatt acgtattatc tcgaccgccc ggacgtgctg gcgcgtttta ccaccaaaat
    10201 tattgccgat aaaatcaaat atcccttcct gctttccaat ggtaaccgcg ttgcgcaagg
    10261 cgaactcgaa aacggacgcc attgggtaca gtggcaggac ccgttcccga aaccgtgcta
    10321 cctgtttgcg ctggtggcag gcgactttga tgtactgcgc gataccttta ccacgcgttc
    10381 tggtcgcgaa gtagcactgg agctgtacgt cgatcgcggc aaccttgatc gcgcgccgtg
    10441 ggcgatgacc tcgctgaaaa actccatgaa atgggatgaa gaacgctttg gcctggagta
    10501 tgacctcgac atctatatga tcgtcgcggt ggatttcttc aatatgggcg caatggagaa
    10561 taagggtctg aatatcttta actccaaata tgtgctggcc cgcaccgaca ccgccaccga
    10621 caaagattac ctcgatattg aacgcgttat cggccatgaa tatttccata actggaccgg
    10681 taaccgagtg acctgccgcg actggttcca gctcagtctg aaagaaggtt taaccgtctt
    10741 ccgcgatcag gaattcagct ctgaccttgg ttcccgcgca gttaaccgca tcaataatgt
    10801 gcgcaccatg cgcggattgc agtttgcgga agacgccagc ccgatggcgc acccaatccg
    10861 cccggacatg gtcattgaga tgaacaactt ctacaccctg accgtttacg agaagggcgc
    10921 ggaagtgatc cgtatgatcc acaccctgct gggcgaagaa aacttccaga aagggatgca
    10981 actctatttc gagcgtcatg atggtagcgc agcgacctgt gacgactttg tgcaggcgat
    11041 ggaagatgcg tcgaatgttg atctctccca tttccgccgt tggtacagcc agtccggtac
    11101 gccggttgtg accgtcaaag acgactacaa tccggaaact gagcagtaca ccctgaccat
    11161 cagccagcgc acgccagcca cgccggatca ggcagaaaaa cagccgctgc atattccgtt
    11221 tgccatcgaa ttgtatgaca acgaaggcaa agtgatcccg ttgcagaaag gtggtcatcc
    11281 ggtgaattcc gtgctgaacg tcacccaggc ggaacagacc tttgtttttg ataatgtcta
    11341 cttccagccg gtgcctgcgc tgctgtgcga attctctgcg ccagtgaaac tggaatataa
    11401 gtggagcgat cagcaactga ccttcctgat gcgtcatgcg cgtaatgatt tctcccgttg
    11461 ggatgcggcg caaagtttgc tggcaaccta catcaagctg aacgtcgccc gtcatcagca
    11521 agggcagccg ctgtctctgc cggtacatgt ggctgacgct ttccgcgcgg tgctgctcga
    11581 tgagaagatt gatccggcgc tggcggcaga aatcctgacg ctgccttctg tcaatgaaat
    11641 ggctgaactg ttcgatatca tcgacccgat tgctattgcc gaagtacgcg aagcactcac
    11701 tcgtactctg gcgactgaac tggcggatga gctgctggct atttacaacg cgaattacca
    11761 gagcgagtac cgtgttgagc atgaagatat tgcaaaacgc actctgcgta atgcctgcct
    11821 gcgcttcctt gcttttggtg aaacgcatct ggctgacgtg ctggtgagca agcaatacca
    11881 tgaagcaaac aatatgaccg atgcgctggc agcgctttct gcggcggttg ctgcacagct
    11941 gccttgccgt gacgcgctga tgcaggagta cgacgataag tggcatcagg acggtctggt
    12001 gatggataaa tggtttatcc tgcaagccac cagcccggcg gcgaatgtgc tggagacggt
    12061 gcgtggtctg ttgcagcatc gctcatttac catgagcaac ccgaaccgta ttcgttcgtt
    12121 gattggtgcg tttgcgggca gcaacccggc agcgttccat gccgaagatg gcagcggtta
    12181 ccagttcctg gtggaaatgc ttaccgacct caacagccgt aacccgcagg tagcttcacg
    12241 tctgattgaa ccgctgattc gcctgaaacg ttatgatgcc aaacgtcagg agaaaatgcg
    12301 cgcggcgctg gaacagttga aagggctgga aaatctctct ggcgatcttt acgagaagat
    12361 aactaaagca ctggcttgat acttttgagc caaaatgcct gatgcgacgc ttgacgcgtc
    12421 ttatcaggcc tacaactccg ttgaaattgc tggttttgta ggctggataa ggcgttcacg
    12481 ccgcatcagg cagccgtgcc caactgcctg atgcgatgct tgacgtgtct tatcaggcct
    12541 acaagtccgt tgaaaatgct ggttttgtgc gccacatccg gcatattaac catgacgccg
    12601 aatcagttgt tcagaatgcc cacgccgcat tacccgttgc aatacttccg cttccagctc
    12661 cgctaatcgc accgacccta atcgacgcgg acgggggata tccactgtca aatccggacc
    12721 aatttttccc tcttcaatta acaacacccg gtcagccatt gctaccgctt cgctcacatc
    12781 atgcgtcacc aacagtacgg taaagccgtg ctgctgccaa agtgacacaa tcaaatcctg
    12841 catctcgagt cgcgttaagg cgtccagtgc cccgagcggt tcatcaagca acaataaacc
    12901 cggtcgatga atcaacgctc ttgccagcgc cacacgctgt ttctgcccgc cagaaagtgc
    12961 ggcaggccat tccccggcgc gattctccag tcctaccgca gccaacgccc gacgcgcggc
    13021 atcgcgccac tgacctttaa ggcctaaccc aacgttatca atcaccgatt tccacggcag
    13081 cagacgcgca tcctgaaaca tcattcgcgt atcatcctga atttcagcca gcggtgtggt
    13141 gcccgctaac acatcgcctg cggttggcgt ttccagaccg gccagcaggc gcagcagggt
    13201 acttttacca ccaccgctgc ggcccaccac cgccacaaac tgacctgccg gaatatgtaa
    13261 atccagttgg ttcaggacga tattttccgc gtaatgtttg cttactgcat tgagcaacaa
    13321 tggtgtgccc tggttcagac gagcagtatt catatcgtgg cctccttcaa atgataagcc
    13381 gggttccagc gcaaccagag gcgctctaac agctgcgcgc tgacgtcagc aagtttgccg
    13441 agcagggcgt aaagaataat agcgactacc accacgtctg tttgcaaaaa ctctcgcgca
    13501 ttcatcgcca gataaccaat gccggaattg gcagaaatgg tttcggcaac aatcagcgtc
    13561 agccacatca ggcccaacgc aaaacgcacg ccgaccataa ttgagggcag ggcatcgggc
    13621 aggatcacat ggataaacag cggtatgccg gataatccat agctacgtgc catctccacc
    13681 agcccgcgat cgatattacg gatgccatgc caggtgttga tataaatggg gaacagcgta
    13741 ccgagcgcca ccagaaagat tttcgcggat tcatcaatgc caaaccacaa aatcaccagc
    13801 gggatcagcg ccagatgcgg cacgttgcgc aacatctgaa ttgaggtatc cagcagcctt
    13861 tctccccagc gcgacagtcc gctaatcagt cccagaatca ggcccagcga tccgccaatt
    13921 gaaaagccaa tcagcgcccg ccaggaactg atcgccagat gctgccacag ttcgccgctg
    13981 gcggagagcg tccagaacgc cgtcaccact ccttccggtg aaggcaaaat acgcgtcgac
    14041 agccagccaa ccgaggaggc cagttgccac accgccacga tgcccaccgg taaaaaccag
    14101 ggggcaacgc gcaataacca cttcttcact ggcgttgcca tcatcgtgtt ccttagcttt
    14161 gcgcgacttt acgggggata aaatcattcg ccaccgcttc gccttgcgga ttcagcggct
    14221 gcggctgggg aatttccgga atggcgacat ccagatgtgg gaacagcaac tcgccaaccc
    14281 gatacgcttc ttccagatgc gggtagcccg aaagcacaaa gctgtcgatg ccaagcgcgg
    14341 cgtattcgtt gattcgcgcg gcgaccgtag gaccatcgcc caccagcgcc gttccggcac
    14401 cgccgcgcac taagccaacg cccgcccata aattggggct gatctccaga ttgtcgcgct
    14461 tgccgttatg tagcgccgcc attcgctgtt gtcctacgga atccgtccgg gcgaatgcgg
    14521 cttgtgcttt ggcgatagtc tcatcatcaa gatgcgagat taaccgctcg gcggcctgcc
    14581 acgcttcgtc gttagtttca cgaacaatca catgcagacg aataccgaaa cgaattttgc
    14641 gcccatacgc ggcagctttc gcccgcactt gttcgatttt ctctttaaca agttccggcg
    14701 gttcgcccca ggtgaggtag agatcaactt gttctgccgc cagctcctgg gcgacatctg
    14761 acgatccgcc aaagtaaagc ggaggataag gctgttgaat cggcgggaag agcagttttg
    14821 ctccgcgcac atgaatatgt ttaccgttga aattgacggt ttcccccagc aataagcgcc
    14881 gccagacttg ggtaaattcc gccgaggctt cgtagcgctc gctatgatca aggaacacgc
    14941 cgtcgcctgc cagctcttgt ggatcgctgc ctgtgaccag attaaacaac gcacgaccat
    15001 ttgagagacg gtcaagcgtg gcggcctggc gggcggcaac agtaggtgag gttacgctgg
    15061 gacgcagggc gacaagaaac ttcagccgct gcgtcaccgg gatcatcgac gctgcaacca
    15121 gccacgcatc ttcacacgat cgccccgttg ggatcagcac accggtatag cccagacgat
    15181 ccgccgcttg cgcaatttgt tgcagataac cgtgatcaac cgggcgtgaa ccttcttccg
    15241 ttcccagata gtgcccgtca ccgtgggtcg gtaaaaacca gaacatattc agactcataa
    15301 ttgttttcct tccagttgag tgggctgcca gatgcgctgg cgaatatcga cttttttcgg
    15361 caccagacgg ttttcataaa acagatctgc cgtttgctgc tgtaaggcgg caacctcggc
    15421 gttaaccggt ttgatggtgg taggagggcg atgatctaag taagaggcaa tcaccggtgc
    15481 cggtaagccc atcgtttttg ccagtaaagc gatgctttgt tcgcgctggc tgcgggttaa
    15541 cgcatcggcc tcactaaagg ttgccagtac gccctgaata aaagcgccgt ttttttctgc
    15601 atagggacga gctgccagat aaaacgatcc agtttgattg agatcggtgc cgtctttcag
    15661 cacccgcacg ccgccctgta ataacgcagc ggagtagtag ggatcccaga tagcccaggc
    15721 gtcaacgtta ccttgctgga acgcggcgcg ggcatcggca ggcgtcaggt aagtgggctg
    15781 gatatcggtg aatttaagtc cggcctgacg cagtgcacgc agtaaaaggt tgtgtgaact
    15841 ggaacctttc tgaaaggcaa ctttgtgacc tttaagatcg gctacggttt tgatcgggct
    15901 gttttctgcc accagaatca cttcggcttt gggcttcggt ggctcgacgc ccacatatac
    15961 caaatcagcc ccggcagcct gggcaaagat tggcggaata tccccggtac tgccgagatc
    16021 aatactgcca acgtttaacg cttccaacat ttgcggaccc gcggggaact ccacccagga
    16081 gatttttgat tgcggatagc gtttttccag taactggtgg ctttttgcca gcaccatacc
    16141 aatactgcct ttctgatagc ctgtacgtaa cgcttcaggc gaggattctg cagcaaccgc
    16201 aaaagtagag acgctaagca atcccgccag cgccagttta atgatgttac gcatgggcat
    16261 tacctcgcag agacagaagg tcaggaacct gaacatcgcg gcggtgcaat gcctgccaga
    16321 aagtttctag cgcggtatca agatgggttt gcagatttgg cgtgaactgg ggtctgtgat
    16381 ggtaatcaat taattgtgag tcatcggcaa acacgccgtg caggatctcc tgagctttca
    16441 gtgcgcttaa aactggttta agggcataat cgaccgccag cagatgggcc acggtaccgc
    16501 ccgtcgccag cggtagcaac actttgcctt gcaaagcgcg ctctggcagc aggtcgagca
    16561 gggttttcag cgcgccggaa taggcggctt tatacacagg cgtggcgaca atcagcccat
    16621 cagcctgttg cagctgttcg gtgaaggtct tgagtgccgg actatcgaaa cgagcataaa
    16681 gcagatcttc cggggcgaag ttttgcagat tccagtgata aacctctaca tctaggccat
    16741 ttagtttttc ccgcgcatat tccagcaagg agctggagcg agaaggaaag cgaggactac
    16801 ccgccagggt gatgacacgc atactctctc cttataacca attgttcttt ttttgttaac
    16861 attgataaca attcggtcag tctgtcggag agacaagaa
//
LOCUS       DADLSA010000085        16808 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.085, whole genome shotgun sequence.
ACCESSION   DADLSA010000085 DADLSA010000000
VERSION     DADLSA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.085"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..597
                     /locus_tag="JN691_002675"
     CDS             <1..597
                     /locus_tag="JN691_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY7244086.1"
                     /translation="GAKYLEITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAG
                     VIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMT
                     LTGLALVIIVNAALMSERPIWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPV
                     RCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(633..1589)
                     /locus_tag="JN691_002676"
     CDS             complement(633..1589)
                     /locus_tag="JN691_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAY7244087.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNHVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1589..2830)
                     /gene="clsB"
                     /locus_tag="JN691_002677"
     CDS             complement(1589..2830)
                     /gene="clsB"
                     /locus_tag="JN691_002677"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAY7244088.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPDYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2827..3588)
                     /locus_tag="JN691_002678"
     CDS             complement(2827..3588)
                     /locus_tag="JN691_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY7244089.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3721..4131
                     /locus_tag="JN691_002679"
     CDS             3721..4131
                     /locus_tag="JN691_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244090.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(4093..5199)
                     /locus_tag="JN691_002680"
     CDS             complement(4093..5199)
                     /locus_tag="JN691_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7244091.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5210..6343)
                     /locus_tag="JN691_002681"
     CDS             complement(5210..6343)
                     /locus_tag="JN691_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7244092.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGMPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6336..8072)
                     /locus_tag="JN691_002682"
     CDS             complement(6336..8072)
                     /locus_tag="JN691_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7244093.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(8065..9063)
                     /gene="hlyD"
                     /locus_tag="JN691_002683"
     CDS             complement(8065..9063)
                     /gene="hlyD"
                     /locus_tag="JN691_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAY7244094.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPMTVQFGDEAGHE"
     gene            complement(9063..9734)
                     /gene="cecR"
                     /locus_tag="JN691_002684"
     CDS             complement(9063..9734)
                     /gene="cecR"
                     /locus_tag="JN691_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAY7244095.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9963..11327
                     /gene="rhlE"
                     /locus_tag="JN691_002685"
     CDS             9963..11327
                     /gene="rhlE"
                     /locus_tag="JN691_002685"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HAY7244096.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIVARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(11559..12041)
                     /gene="ybiA"
                     /locus_tag="JN691_002686"
                     /pseudo
     CDS             complement(11559..12041)
                     /gene="ybiA"
                     /locus_tag="JN691_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
     gene            12161..14311
                     /gene="dinG"
                     /locus_tag="JN691_002687"
     CDS             12161..14311
                     /gene="dinG"
                     /locus_tag="JN691_002687"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAY7244097.1"
                     /translation="MALTVALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIAKKKEKTKSPRRRRR"
     gene            14339..15301
                     /gene="ybiB"
                     /locus_tag="JN691_002688"
     CDS             14339..15301
                     /gene="ybiB"
                     /locus_tag="JN691_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAY7244098.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15442..16527
                     /locus_tag="JN691_002689"
     CDS             15442..16527
                     /locus_tag="JN691_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAY7244099.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
BASE COUNT         3926 a         4450 c         4552 g         3880 t
ORIGIN      
        1 ggagctaagt atctcgaaat tacggcaacc aaatatgatt caccaccaat gtatgtcgcc
       61 atagggttat tattactttg tatgtatggc ttaagtaagg atatcaacaa gataaatgcc
      121 gccatcgcgg gcgtaatata tctgttttta ctctctttgg tggcgattgt cgttgcaagt
      181 ttagttcctg tatatgccat tatcatcgtg ttcagcactg cgggcgcgat gtttttaatc
      241 agtatgctgg ccggtttatt atttaatgtt gatcctggtt ctcaccgttt tatcattatg
      301 atgacgttga cagggttggc cctggtaatc atcgtgaatg cggcattaat gagtgaacgg
      361 cccatttggg taataagttg cttaatgatt gtgttatggc caggcattat ctcgcatgga
      421 cgaaataagc tccttgaact ggcaggaaaa tgccatagtg aagagttgtg gagtccggtt
      481 cgttgcgctt ttacaggtgc attaacactc tattactatt ttatcggctt ctttgggata
      541 cttgccgcga tagctataac gcttgtctgg caaaggcata cgcgtttttt tcattagtcg
      601 taaatgcaga tactaaagaa cttaactaaa cttcacatcg ccgcttcatt tttcgcccgt
      661 agcttcttcg cctggctttc cagtagcagg taacagatca gcgccagcag cagcgggata
      721 aagtaataca ggacgcgata agcgagcagg gcggcgataa tgctcccttt cgaggtatgc
      781 tccccagcca gtagtgcgat aaacaccgct tccagcacac cgatccccgc cggaatatgc
      841 acgatgacgc cagcaatact actaaccagt aacacgccca gtacaaagaa atagttcacg
      901 ctttgaccaa gtaacagcca gataatcgcc cccattacca tccagttaac gctggaaatc
      961 agcatctgag cgagggcgaa tttccatgaa ggcagcacca gcttttgccc tttgatggtc
     1021 atatggcggt gcttcgcgaa agcgcaaaac cacaaataaa ccgcgataat catcagtaag
     1081 ccaatgccga gaatacgcag cgtagtttga tcgacatacc agtgatccgg caactccacc
     1141 acgcctgcgg taaagataat ccctgccagt aaaatatagc ccagccagtt ggtggtaata
     1201 ctgagcgagg aaatccgcgt aatagtgctg cccggtaacc ctagtcggga gtacaaacga
     1261 tagcgcatac caatgccgcc aacccaggta ctgagcgtca ggttgaaggc gtagcagata
     1321 aacgacacca gcatcacctg gcgcttcgcc agtttgtgac cgcagtaaaa gcgggcgagc
     1381 aggtcatagc agccgtaaat cagatagctg acgaccacca gcccgaccgc actaagcagc
     1441 gcaacgtgat tgtagtcgcg gatgaccttc cagacctctt cccagtccac ttttttggcg
     1501 tagaccacca gcaacacaat caccgcgata aaaaacagcc aggtgaggat cttctttgct
     1561 aagcgccagc gcgggtgtga tttactcatc agggttttac ccccgtgttt tcagtttcta
     1621 cccgatcctg cgtttccatt gtcggttgtg cggggggatc aacctgcgtc agacgtggtg
     1681 tgtgtgccgg aagccagcca accagcgccg ggaagtggcg taaaaagtgg aacgccagca
     1741 cgcttttggt caggttccac caggtgcgtt tgggcagcat ggtttcatcc acctgctgac
     1801 aatctgcggc aataatgccg ttcagattat cgcgcagcgt ctggttaaaa tgacgatcgt
     1861 ggatgatgac atttgcttcg agattcagtg acaaactgag cggatcgaga ttactggacc
     1921 ctactgtcgc ccagtgatcg tccatcaatg ccactttgcc gtggagcggg cggcggcggt
     1981 actcaaaaac ctgaacgccg cctttaacca gatagttata cagcaagcgc gcaccgactc
     2041 tgacaatcgg catatccggt tcgccctgaa tgatcagttt gatccgcacc ccgcgccgtg
     2101 ccgctttacg caaggcgtgt aaaaatcgat agtcggggaa gaagtaggcg ttggcgataa
     2161 tcacttcccg ccgcgcctga gtgagcattt tcagataatg gcgttcaata tcatcgcgat
     2221 gttcttcgtt atcgcgccag accagcaata cctgcgcttc tcccggctgg cggttctctt
     2281 ccgctttgtg atgacgtcgc caccagcgtc gtgccgcgct ctgtccaggc aggttttcca
     2341 gctcaaactg gagaatatct tcgacaatcg gcccttcaag gcgtaccgcg taatcctgtt
     2401 tagcctctgg accgtagctg gacatatgct cggcggagta attcagcccg ccaataaagg
     2461 cgatacgcgc gtcgatcacc acaattttgc gatgcatccg gcgaaacaca ttggtgcgca
     2521 taccaaaaag gcgagggcgg ggatcgtagt agcggaacac tacgccagct gccgtcagtt
     2581 cattgacaaa ctcatcgctg agatccggcg aaccgtagcc atccagcaag acttccgctt
     2641 taaccccgcg ttgcgctgct gccagtagtg ccgcatgcag ctgtttgccg acgttatcct
     2701 caaaccagat aaacgtttca agaatgatgc gttcttgtgc ctcgccaatc gccttaaaca
     2761 ccgcgggata atattgctcg ccgttttcca gcaactggat cttattgcct tcgcgccagc
     2821 tacatttcat aaatgaatct ccgcacttaa aggggcatga tcagaaaggt gtcgccatgt
     2881 ccgcagcggc aacgcggttg gcgcgctggc gctggcattt ttgacgtaga tcctgtccag
     2941 tcgtagtaga ggaaattgca ccggaaacgt gcgcgccggg cgtccgtggg cgcgggtaaa
     3001 aatctcatcc agtccggcct gcacttttaa cggatgatta gctttttgcc gccagtcatt
     3061 gaaatcaccc gccaccaata ccggttcgcc gtccggtagc tcattcaccc attcggcgag
     3121 catcgcaagc tgcgcctgac ggtgcgcctc acgcaggccc agatgtacgc acatcacatg
     3181 aatcgctttt ccggtcatcg gcggcacaat gcggcagtag agcacgccgc gcttttccgc
     3241 accatcgacc gaaacatcgc gattctcata atgttcaatg ggataacgcg acagtacggc
     3301 gttgccgtga tgcccttccg ggtatacggc attgcgaccg taggcaaaat cgctccacat
     3361 agtgtcggcg agaaactcgt agtgcgaggt atcgggccag ttttccacat gcagcggatg
     3421 aacttcgtgc gcgcccatca cttcctgcag gcaaacaata tcggcgctga cggtacgcac
     3481 ggcatcgcga agttccggca aaatgaagcg tcggttaaac gcggtaaagc ctttgtgaat
     3541 gttgatggtg agcaccttga acgaaaattg ttgtgtttga tcgggcataa ttttcctgtc
     3601 tttgcctctt atctcattga aatagtgtag tcggcgtcac aaaaaggtgc ggtcttacgg
     3661 aattttccgt aaagttcggt actctgagta agtagagata aattcttcag gagagaagcc
     3721 atgaagtggc aacaacgtgt tcgtgtcgca acgggtctaa gttgctggca gattatgttg
     3781 catttactgg tagtggcgct gctggtggtg ggctggatga gtaagactct ggttcacgtc
     3841 ggcgtgggat tatgcgcact gtattgtgtc acggtagtga tgatgctggt gtttcagcgc
     3901 caccccgagc aacgctggcg tgaggtggca gacgtgctgg aagagctgac cacgacctgg
     3961 tattttggcg cagcgctgat tgtgctatgg ctgttgtccc gcgtactgga aaacaacttt
     4021 ttgctggcaa ttgcagggct ggcgatcctt gctggcccgg cggtagtgtc tttgctggcg
     4081 aaagataaga agttacatca ccttacgtct aaacatcgcg tacgccgctg accctgtcgt
     4141 ggccgttatc accagtagcg gccacaaact attccacaca atatccagac tcgcatcctt
     4201 caaataaatc tgcttggtaa tgtccgtaaa gtggcgaata gggttaatcc acgtcagatt
     4261 ttgcagccac actggcatgt tttccaccgg cgaaacgtaa ccggaaagga gaatggcggg
     4321 catcataaaa acaaacacgc cgataaacgc ctgctgttgt gtcgagcaga gtgatgaaat
     4381 caacagaccg aatcccacca gcgataaacc gtaaatcacc atcgtaaagt agaacagcgc
     4441 tagcgatccg gcgaagggga tttgatacgc ccagatacca atcgccagca caatggtggc
     4501 ctggaaggtg gcgacaatta acgccggtac ggctttgccg atgaagatct gccaggtggt
     4561 gagcggcgaa accagtagct gatcgagcgt accttgttca cgttcgcggg cgacggaaag
     4621 tgaagtgacg atcattacgc cgatagtggt gatcatggcg atcagcgacg gcaccacaaa
     4681 ccatttgtag tcgagattcg ggttatacca gttgcgtacc accagctcgc tgttgttggg
     4741 tttcggtttt ccttccagca gctcctgctg ataatttttg acgatctgtt gcaggtagtt
     4801 ggcggcaatt tgcgcactgt tggagttacg cccgtcgagg atcaactgca acggcgcggt
     4861 ctggaaggta tccagtttgc gcgagaagtc agccgggaaa cgcaccagta gtaacgcctt
     4921 ttgtgtgtcg atggtcgggc ggatctcctg tgggcttttc agcagcagca catgtgtaaa
     4981 ggcgctggcg cgggcaaaac gttgagtaag ttccaccgaa tgctcgccgt tatcttcatc
     5041 gtagatggcg atggtggcgt tagtcacttc cagcgtggcg gcgaacggga acaggatcac
     5101 ctgaattagc acgggtaaaa tcagaatcgc gcgggtttgt ggttcgcgca gcaacgactg
     5161 caactcttta cggattaacg tccataagcg atgaaacatg ctcttctccc taatccagcc
     5221 gacgtttggt tttcagccac gtcaggccga taaacatcac cgccgaagcg atcaaaaaca
     5281 gcacgtttac caccagcacc actggaatat tcccggcgag gaacaggctt tgcagggtgc
     5341 tgacgaaata acgagcggga ataatgtacg tcaccgcgcg gatcaccgcg ggcatactgt
     5401 cgatctgaaa aataaagccg gaaagcataa tcgacggcag aaaagcggcg ttcagggcga
     5461 cctgagcggc attgaactgg ttgcgggtaa tcgtggaaat cagcagcccc attcccaggg
     5521 tactgagtaa aaacaggctg gagataaaaa acagaatcag cagcgaaccg cgatacggca
     5581 tgccgagaat aaacactgac accagcatac acagcaacat cgccagcatc ccgagaaagt
     5641 aataagggat cagcttacac agcagcagtt ccgtgcgggt aatctccgta gagagcagag
     5701 cctccatggt gccgcgttcc cattctcgcg ccaccaccag cgaggtgaga atcgcgccga
     5761 tgaccgtcat gataatggtc accgcacccg ggataataaa gtgctggctt atcgccgccg
     5821 ggttaaacca gtagcgggtt tgtacatcaa taagcggttc aaaagtctgc ccgttgtcct
     5881 ccgctcgctg catttgccag atctgccaga tcccttcgac atacccctgc acaaagttag
     5941 cggtattcgg ctcactgccg tcggtgatca cctgaatcgg tgcggtggcg ttggcgcgct
     6001 ccatctgttc ggcgaaatcc acgggaataa ccaccagacc gcgaattttc cccgactgca
     6061 ttttggcgat cagttcctga cggttatcgc tgatggtggc gtcgatgtag ggcgaaccgg
     6121 tcatggtgtg ggtgaaatcc agcgccgctt cgctacgttg ttccagcaaa atcccgaccc
     6181 gcagcttgct ggagtcgagg ttaatgccgt aaccaaaaat aaacagcagt agcagtggga
     6241 tcactaccgc aatcagccag ctactcggat cgcgaacgat ctgccgcgtc tctttgacgc
     6301 acagcgcccg cacgcgacgc caggaaagga tcgggttact cattgctatg ctccttatcc
     6361 cagtcgtgga tcaactgaat aaaggcttgc tccatggtgg gatcgggttg ctcatcgtta
     6421 gctgactgtg ctttcaaatc gtccggcgta ccgctggcga ttaatttccc acggtacacc
     6481 aggccgatgc ggtcgcaata ttccgcttca tccataaagt gggtggtgac catcaccgtg
     6541 acgcctttct ctaccatgct gttgatgtgc agccaaaatt cacggcgggt gagggggtca
     6601 acgccggaag tcggttcgtc gagaaagaga atatccggtt catgcatcag cgaacaggcc
     6661 agcgccagcc gctgtttaaa acctaatggc agttcatcgg tggcgtgtga ggcaatactt
     6721 ttcaggccaa atgcctcgct catacgggag attttttcgt tctgcgcccg accgcgtaag
     6781 ccatacacac cagagaaaaa gcgtaaattc tgttcgaccg tcaggttgcc gtagagcgaa
     6841 aatttttgcg ccatatagcc gagatgctgg cgcgctttac cggaactctc tttcagatcc
     6901 atccccagca ccagcgcctg gccggaagtc ggcaccagca aaccgcacat catcttaaag
     6961 gtggtcgatt tacccgcgcc gtttggcccc agcaaaccaa aaatctcccc acgtttaacg
     7021 gcaaagttga cgtgatcggt ggcggcaaaa tcaccaaatt tcttggtcag ttctttcgct
     7081 tcgatcaccg tctcgccggg tgtgccttct accgtatgca atattgcacc cagcggcgat
     7141 tctgaggttc cggcaccgcc cagcaaatca ataaacgcat cttcaaaacg cggcgtagtt
     7201 tcgttgatgt tgatttccgg catcccgtcg gcatggcgaa tatcgtctgg tgtggcctct
     7261 tttttgagga tcagacgtac cgatttcccc tgaatcatgc cgtcgctgac ctgcggcagt
     7321 ttcaaggcgc gttgcaacag tttgcggttg ccctcgtgtg gactggtcat cagaaagctg
     7381 cgtccggcca tggtttgtgt cagggctttt ggttctccct gatacagcaa ctcgccttcg
     7441 ttcatcagta acacgtcacg gcactgctcg gcttcgtcga gatacgaggt actccagagg
     7501 attaacatcc cttcgcccgc cagctcatgc accatctgcc acagttcgcg ccgtgagata
     7561 gggtcaacgc cgacgccggg ttcatcgagc agcaacactt tcggttcgcc caccagggta
     7621 caggccaggc cgagtttttg tttcatccca ccggagagct tgcccgccag gcgtccggta
     7681 aacggcccaa gagacgtaaa ctccagcagg cgagcaaaag tttgcttacg tgcctcgccg
     7741 gtgacgctgc gcaaatccgc gtacagattg aggttctcca tcaccgtgag atcttcatac
     7801 agaccaaatt tctgcggcat atagccgagc acggcgtgca gcgcgccgtc gtttttgatc
     7861 ggatcaaagc caatcaccgt ggcactgccg ctgtcgggtt tcagtaatcc cgccaacatc
     7921 cgcatcagcg tggttttacc tgcaccgtcc ggccccacca accccgtcac ataaccggcg
     7981 tgaatggtac aatcgagcgg cgcgacggcg ggcttgtcca tgcccggaaa gcgtttttcc
     8041 aggccgttca gcgtgataac ggcatcattc atgtcctgcc tcgtcaccga attgtaccgt
     8101 cattggcatt ccctggcgta acgcatcatc ggcgtcggtc accacaatac gcaggcgata
     8161 gacgaggtcg gtacgcagat ccggtgtttc gacggttttc ggggtaaatt cagcagtcgg
     8221 cgaaacgaaa ccaatctgcc cgtgatacgg cttgtccggg cgaccatcgg tataaagcag
     8281 cactttgcgc cccggctggg cctggtcaag attccgttca tcaacataag cgcgcaccca
     8341 caccggacgc gttagtgaaa cggtaaacac cgtgccacct tcattgagaa ccgtgcctgg
     8401 ctctaccgcg cgcgttaaca gcgtgccatc agacggtgct atcaacgttg agtcctgtaa
     8461 attcagctcc gcctgcgcca gttgcgcctg cgcctgttcg aggctggctt tcgcctgagc
     8521 gatgtcctgt tcacggttac cggaacggta ctgacgcaat ttatcctgtg ctgatttcag
     8581 cgttgcctgt gcctggtcgc gcgaggagcg ggcattttcc aggtcatttg ccgaaatggt
     8641 gcggcttttc cacagtcctt gctggcggtt atagaagttc tgcgcatagt cataggcggc
     8701 ttgcgcctgt ttcaccgctg cggcggcctg agcgatttct tcatcgcgat acccggcaag
     8761 catcagatca tactgcgcct gtgccaccga gacgcccgct ttcgcctgca tcagggcaat
     8821 ctcatacggc ttgtgatcca gttcgcccag cacctggccc gctttgatag catcaccttc
     8881 gtccaccgcc agcgattcaa cgcgcccccc aacacggaaa ctaagattca ccgtacgaat
     8941 atccacgttg ccatacagcg tcaggccgtt atcctggcgg ctttgatacc accagtagcc
     9001 tccggcaacc acggcggcaa gtaccactac cgccagtccg atcacgacag gttttttcat
     9061 cactacaaac tcctttgcga taatccttgc aaaatcaggt cgatatgaca ggtcaccgtc
     9121 tggttgatca gttcggtctt ttcttcatcg aacgcggtcc agccggtacg taacagaatt
     9181 gtttctttgc caagacggaa cgccagaatc tcaccaatca acgcatgggt atgaaggatc
     9241 atgcgggtgt cattagcgtc gcagccggtc caggcggcaa tcaggcgtgt caggtggctg
     9301 tgtagcggac taatcacctg ttcgtgcacc aggtggtagg ctgccgtggg agagagctgc
     9361 tcacgggaga taaacttgct gaggttgacg gtgtcatcct gggtgagcag cttaatcatg
     9421 ttcctgcagg cgcgaaggat cagttcacgg atggcagccc gatcaggctg tggttgggcg
     9481 aacaagcgtt cggcttcctc ggcatgcgga cggaactgct cgccaataaa atcggcaatc
     9541 cactgggcgc aggcgaggta caaatcttct ttcgaaccga agtagtaggt gatggcagcg
     9601 atattctgcc cggcctgggc ggctatctcg cgggtggtgg cgttcattcc atattcacca
     9661 aactgcgcca gtgcggcagc aatcagctgt tttttcgcct gttcaccctt gattgtcatg
     9721 gcaggattat tcatcgcaca gtccattctt aatcaaatga ttgattaaga ttatgactcc
     9781 atatgggagt tgtccagtat ggctaagaat tttagctacg ccagtcacag ggataattta
     9841 tgcgctgcgt cacaaaaact gctacactcc gctccctcat gacattgtgg tttttgtcat
     9901 tttccttttc agtatctccc tgaaaactac accggtaacg gtcggggcgg ttcggagtag
     9961 ttatgtcttt cgattctttg ggtttaagcc ctgatatcct gcgcgccgtt gccgagcagg
    10021 gttaccgtga acccacccct attcagcagc aggcgatccc tgcggtgctg gaaggccgcg
    10081 acctgatggc tagcgcccag accggcaccg gcaaaacagc gggctttacg ctgccgctgt
    10141 tgcaacacct gatcactcgc cagccgcacg ccaaagggcg tcgtccggta cgtgcgctca
    10201 ttcttacccc gacccgtgaa ctggcggcac agattggcga aaacgtccgt gattacagca
    10261 aatacctgaa cattcgttcg ctggtggtgt ttggcggtgt cagcattaac ccacaaatga
    10321 tgaagttgcg cggcggcgtt gatgtgctgg tggcaacgcc gggacgtttg ctggacctgg
    10381 aacatcaaaa tgcagtgaag ctggatcagg ttgaaatcct cgtcctcgat gaagctgacc
    10441 gcatgctcga catgggcttt atccacgata tccgtcgcgt gttaacaaaa ctgccagcga
    10501 aacgccagaa cctgctgttc tctgcgacct tctctgacga tattaaagcc ctggcggaaa
    10561 aactgttgca caacccgctg gaaatcgaag tggcacgccg caataccgcg tctgatcagg
    10621 tgacccagca cgttcacttt gtcgataaga aacgcaaacg cgaattgctg tcgcacatga
    10681 ttggtaaagg gaactggcag caggtgctgg tgtttacccg taccaaacac ggcgctaacc
    10741 atctggctga gcagctcaat aaagatggca tccgtagtgc ggcgatccac gggaataaat
    10801 cgcaaggtgc gcgtacccgt gcgctggctg attttaaatc gggtgacatt cgcgtactgg
    10861 tggcgaccga tatcgttgcg cgcggtctgg atatcgaaga gttgccgcac gtcgtcaact
    10921 atgaactgcc aaacgtaccg gaagattatg tccaccgtat cgggcgtacc ggtcgtgcgg
    10981 ctgctaccgg tgaagcgttg tcgctggtgt gtgttgatga acacaaactg ctgcgtgata
    11041 tcgaaaaact gctgaaaaaa gagatcccgc gcattgcgat tccgggctat gagccggacc
    11101 cgtcaatcaa agccgaaccg atccagaacg gtcgccagca acgtggcggc ggcggtcgtg
    11161 ggcaaggtgg tggtcgcggt caacagcaac cacgccgtgg ggaaggtggc gcaaaatctg
    11221 caagcgcgaa acctgcagaa aaaccgtctc gccgcctcgg cgatgccaaa ccggcaggcg
    11281 aacaacaacg tcgccgccgt ccgcgtaaac ctgccgctgc gcagtaatct tttatgccgg
    11341 gctatgcccg gcatcaggct gatgaacaaa cgcaaaactg cctgatgcgc tacgcttatc
    11401 aggcctacgt gaactctgca atatattgaa tttgcatgct tttgtaggcc ggataaggcg
    11461 tttacgccgc atccggcatt tcacaacaag cacttgtcag caatttgaga acacgggaaa
    11521 aaatttatct gttttaccgc ccataagcca ccgagacgtt acttctctat agccaattgt
    11581 tcgcgcaact ccattaaaag gtagcccagt cgattcttgc ccttaccatt accaccgtct
    11641 ccccagtaag catcgttttc cgtatgctca accagttttg cgggcgcggt tgccagcaag
    11701 agcacacgca gttctgcatg ctgttcgaat ttagcgcgaa gtgctttacg catcacctgt
    11761 tctttaaccg actcccagtt tttacgaagt ggcttggaac gattacgccc catgcgagcg
    11821 gcgaccatgg gggaagaaac ccgacggatc tcttcgcggt atttttcatc aaggaatttt
    11881 tgtgcctgaa aatagtgttc ggaggtgggc taggtattcc cgtcaacctt gatgggccag
    11941 gcggcaaagt tagaaaaatc accgtagtca tcgctggtgc tgtagaaatt tatgatggtg
    12001 tcttgcatga cgtgttggat cctttgtgct cgaacgggca ttaaaccgca ttatgttggt
    12061 ggttattgcg agccgctttc cagaaacaga aaaaccatta cccctgaaaa ccgaaaaatg
    12121 ccacaatatt ggctgtttat acagtatttc aggttttctc atggcattaa ccgtcgcgct
    12181 taaagcgcaa attgccgcct ggtataaggc gcttcaggaa cagatccccg actttattcc
    12241 ccgtgcgccg cagcggcaga tgattgcgga cgtcgccaaa acgctggccg gagaagaagg
    12301 gcggcatctg gcgattgaag cccccaccgg cgttgggaaa acgctctcct atttgatccc
    12361 cggcatcgcc attgcccgcg aagagcaaaa aacgctggtg gtgagtaccg ccaacgtggc
    12421 gttgcaggat cagatttaca gcaaagattt accgctgctg aaaaagatca ttcccgatct
    12481 taaattcact gccgctttcg ggcgtgggcg ctacgtttgt ccgcgtaatc tgacggcgct
    12541 cgccagcact gaacccacgc aacaggatct gctggcgttt cttgacgacg aactgacgcc
    12601 aaataatcag gaagagcaaa aacgttgtgc gaagctgaag ggcgatctcg acacttataa
    12661 atgggatggt ctgcgcgatc atacggatat cgctatagat gatgatctct ggcgtcgttt
    12721 gagtaccgac aaagccagct gcctcaaccg taactgttac tactatcgtg aatgcccgtt
    12781 ttttgttact cgtcgggaga ttcaggaagc ggaagtggtg gtggcaaacc atgcgctggt
    12841 gatggcggcg atggaaagcg aagccgtact gcctgacccg aaaaatctgc tgctggtgct
    12901 ggacgaaggt catcacctgc cagatgtggc ccgggatgcg ctggagatga gcgctgaaat
    12961 caccgcgccc tggtatcgac tacagctgga cttgttcacg aaactggtcg ctacctgcat
    13021 ggagcagttt cgcccgaaga ccatcccacc gctggcgatc cctgaacgtt tgaatgcgca
    13081 ttgtgaagag ttgtatgagc ttatcgcctc gttaaacaac attctcaatc tctacatgcc
    13141 tgccgggcag gaggcagagc accgttttgc gatgggcgaa ctgcctgatg aattgctgga
    13201 gatctgccag cggctggcaa aactcaccga gatgctgcgt ggtctggcgg agttatttct
    13261 taacgattta agtgagaaaa ccggcagcca cgatattgtg cgtctgcatc ggttgatttt
    13321 acagatgaac cgcgcgctgg ggatgtttga ggtgcaaagc aaactctggc ggctggcgtc
    13381 tctggcgcaa tcttccggtg cgccggtgac caaatgggcg acgcgggaag agcgcgaagg
    13441 gcagctacac ctctggtttc actgcgtggg aatacgtgtc agcgatcagc tggaaaggct
    13501 gctgtggcgc agtattccgc acattattgt cacctccgca accttgcgtt cgctgaatag
    13561 tttttcgcgt ttgcaggaga tgagtggtct gaaagagaaa gcgggcgacc gttttgtggc
    13621 gctggattcc ccctttaacc actgcgaaca gggcaaaatt gttattcccc ggatgcgctt
    13681 tgagccttcc atcgacaacg aagagcaaca cattgccgaa atggcggcct ttttccgtga
    13741 acaggtggag agcaaaaaac atctcggtat gttggtactg tttgccagcg gacgggcgat
    13801 gcagcgcttt ctcgactatg tgacggattt acgcctgatg ttgctggtgc agggcgatca
    13861 gccgcgttat cgcttagttg aactgcaccg caaacgcgtt gccaacggtg agcgtagcgt
    13921 gctggtgggc ttacagtcat ttgccgaagg gcttgatttg aaaggtgatc tgctcagcca
    13981 ggtgcatatc cacaaaatcg cttttccgcc catcgacagc ccggtggtga tcaccgaagg
    14041 ggaatggctg aaaagcctca accgctatcc gtttgaggtg caaagcctgc cgagcgcctc
    14101 gtttaacctg attcagcagg ttgggcgact gattcgaagc cacggttgct ggggcgaagt
    14161 ggttatctac gacaaacgct tgctgacaaa aaattatggc aagcgactac tggatgcatt
    14221 accggtattt ccgatagagc aaccggaagt ccctgaaggt atagctaaaa agaaagaaaa
    14281 aacgaaatcc ccacgccgtc ggcggcgtta atgatgtgag tcaggtaagg agtcgtaaat
    14341 ggactatcgc aaaatcatta aagagatcgg gcgcgggaaa aaccacgcgc gcgatttaga
    14401 ccgggatact gcgcgcggtc tgtatgctca tatgctcaat ggtgaagtcc ctgaccttga
    14461 gttgggcggc gtattgattg cgctgcgtat caaaggggaa ggggaagcag agatgctcgg
    14521 cttttacgaa gccatgcaaa atcacaccat caagctgacg ccgcctgcag gcaagccgat
    14581 gccgattgtc atccccagtt acaacggcgc gcgtaaacag gctaacctga cgccattatt
    14641 ggcgattctg ctgcataaac tcggttttcc tgtggtggtt cacggagtta gcgaagatcc
    14701 aactcgcgtg ctgacagaaa ccattttcga actgatgggt attacaccaa cgttgcacgg
    14761 cgggcaggcg caggcgaagc tcgacgaaca tcaaccggtg tttatgcctg tcggcgcgtt
    14821 ttgcccgccg ctggaaaaac aactggcgat gcgctggcgg atgggcgtgc gtaacagtgc
    14881 acataccctg gcgaaactgg cgacgccatt tgccgaaggt gaggcactgc gtctttccag
    14941 cgtttcgcat ccggaataca ttggacgcgt cgcgaagttc tttagcgata tcggcgggtg
    15001 ggcgttacta atgcatggta ctgaaggcga agtgtatgct aatccgcagc gctgcccgca
    15061 gatcaatctc attgaccgtg aagggatgcg ggtgctgtat gaaaaacagg accctgctgg
    15121 tagcgagtta ctgccacaag caaaagatcc ggaaaccacg gcgcagtgga ctgagcgttg
    15181 cctggctggc agcgaagcga ttcccgaatc gctgaaaatc cagatggctt gctgcctggt
    15241 ggctacgggt gaagcggcaa ctatcagcga tggcctggcg cgcgttaatc aggcattttg
    15301 atttttatct cagccggatg agccacgtca tccggctttt accccaccgc aatatgaaat
    15361 tcctgcatct ttattgacct tcccgcgccc ggcgtgcagc ataaaaaaac aacaaacaca
    15421 taacataaac aggagttaac catggaaagt ggtcatcgct ttgatgctca gacgctgcac
    15481 agttttattc aggctgtatt tcgtcagatg ggtagcgagg aacaagaagc gaaattagtt
    15541 gccgatcatt taatcgcggc aaacctggca gggcatgatt ctcatggtat tggcatgatc
    15601 ccaagctatg tgcgttcctg gagtcagggg catctgcaaa ttaaccatca tgccaaaatc
    15661 gttaaagagg cgggggcggc agtcacgctc gatggcgatc gcgcattcgg tcaggtcgcg
    15721 gcacacgaag cgatggcgct ggggattgag aaagcgcatc agcacggtat tgctgccgtg
    15781 gcgctacata actcgcatca tatcggccgt atcggttact gggcggagca gtgttcagcg
    15841 gcggggtttg tctctatcca ctttgttagc gtggtcggta ttccaatggt cgcgccgttc
    15901 cacggtcgcg acagccgctt tggcaccaat cccttctgtg tggttttccc tcgtaaagat
    15961 aatttcccgc tgttgcttga ttacgccacc agcgccattg catttggcaa aacccgcgtc
    16021 gcctggcata aaggcgtccc cgtgccgcca ggttgcctga ttgacgttaa cggcgtgccg
    16081 acgaccaatc cggcggtaat gcaggagtcg ccgttggggt cgctgttgac ctttgccgaa
    16141 cataaaggct acgcccttgc agcgatgtgt gaaattcttg gcggggcgct ttctggcggt
    16201 aaaacgacgc atcaggaaac gttgcaaacc agccccgatg ccattcttaa ctgcatgacc
    16261 actatcatca tcaacccgga actgttcggc gcgccggatt gtaacgcgca gaccgaagcc
    16321 tttgccgagt gggtgaaagc ctcgccgcat gatgacgata agccgatttt gctaccgggc
    16381 gagtgggaag tgaacacgcg tcgcgaacgg caggagcagg ggattccatt ggatgcggga
    16441 agctggcagg ccatttgtga tgcggcgcgg cagattggta tgtcggaaga gacgttgcag
    16501 gctttctgtc agcagttagc cagctaaaaa aaagcccgtc cagtggcgga cgggcaaaca
    16561 agggtaacat aggatcaatg agggttagag catatgcgtc tgtcggcaaa cagacaggga
    16621 aatacttgtg ctggacgtag cgtaaacgcc tgatccgtcc aacggttcgg attttgtagg
    16681 cctgataaga cgcggtagcg tcgcatcagg catctgttgc cggatgcggc ataaacgcct
    16741 tatccgtcct acgaattact tataaattac cgcagtaccg cttaatttat tgttgttggt
    16801 ggcggaag
//
LOCUS       DADLSA010000086        16281 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.086, whole genome shotgun sequence.
ACCESSION   DADLSA010000086 DADLSA010000000
VERSION     DADLSA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.086"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            7..1737
                     /locus_tag="JN691_002690"
     CDS             7..1737
                     /locus_tag="JN691_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244100.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQLEKAMQLPSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVMPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYATNGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKLL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(1797..2288)
                     /gene="yajQ"
                     /locus_tag="JN691_002691"
     CDS             complement(1797..2288)
                     /gene="yajQ"
                     /locus_tag="JN691_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAY7244101.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2456..3367
                     /gene="panE"
                     /locus_tag="JN691_002692"
     CDS             2456..3367
                     /gene="panE"
                     /locus_tag="JN691_002692"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY7244102.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3330..3920
                     /gene="yajL"
                     /locus_tag="JN691_002693"
     CDS             3330..3920
                     /gene="yajL"
                     /locus_tag="JN691_002693"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAY7244103.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3974..5422)
                     /gene="thiI"
                     /locus_tag="JN691_002694"
     CDS             complement(3974..5422)
                     /gene="thiI"
                     /locus_tag="JN691_002694"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAY7244104.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5628..5870
                     /gene="xseB"
                     /locus_tag="JN691_002695"
     CDS             5628..5870
                     /gene="xseB"
                     /locus_tag="JN691_002695"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAY7244105.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5870..6769
                     /gene="ispA"
                     /locus_tag="JN691_002696"
     CDS             5870..6769
                     /gene="ispA"
                     /locus_tag="JN691_002696"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAY7244106.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6794..8656
                     /gene="dxs"
                     /locus_tag="JN691_002697"
     CDS             6794..8656
                     /gene="dxs"
                     /locus_tag="JN691_002697"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAY7244107.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSCKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8711..9685
                     /gene="yajO"
                     /locus_tag="JN691_002698"
     CDS             8711..9685
                     /gene="yajO"
                     /locus_tag="JN691_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAY7244108.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9739..10257)
                     /gene="pgpA"
                     /locus_tag="JN691_002699"
     CDS             complement(9739..10257)
                     /gene="pgpA"
                     /locus_tag="JN691_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAY7244109.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10235..11212)
                     /gene="thiL"
                     /locus_tag="JN691_002700"
     CDS             complement(10235..11212)
                     /gene="thiL"
                     /locus_tag="JN691_002700"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAY7244110.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKSGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11290..11709)
                     /gene="nusB"
                     /locus_tag="JN691_002701"
     CDS             complement(11290..11709)
                     /gene="nusB"
                     /locus_tag="JN691_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAY7244111.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11729..12199)
                     /gene="ribE"
                     /locus_tag="JN691_002702"
     CDS             complement(11729..12199)
                     /gene="ribE"
                     /locus_tag="JN691_002702"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAY7244112.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLE
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12288..13391)
                     /gene="ribD"
                     /locus_tag="JN691_002703"
     CDS             complement(12288..13391)
                     /gene="ribD"
                     /locus_tag="JN691_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAY7244113.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRYVGPDVCLHLVGA"
     gene            complement(13395..13844)
                     /gene="nrdR"
                     /locus_tag="JN691_002704"
     CDS             complement(13395..13844)
                     /gene="nrdR"
                     /locus_tag="JN691_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAY7244114.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13995..14534
                     /locus_tag="JN691_002705"
     CDS             13995..14534
                     /locus_tag="JN691_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY7244115.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14833..15717
                     /locus_tag="JN691_002706"
     CDS             14833..15717
                     /locus_tag="JN691_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAY7244116.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15894..16241)
                     /locus_tag="JN691_002707"
     CDS             complement(15894..16241)
                     /locus_tag="JN691_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAY7244117.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
BASE COUNT         4180 a         4163 c         4034 g         3904 t
ORIGIN      
        1 tttgcgatga aagcgaaatc tactttgtta ttaatgattt tatctgtttt ttattctcat
       61 caggttttat ccagcgaacc cactcagccg gatgtttatt ctgtcgtcga gaaaaaacta
      121 aactctgctt tacctctgga agaaaatacc accttcagat cgcaggctga atggtttaat
      181 ttgcaatatg aattattagt atctggctac cctgaacgtg cttacaattt actgagcaaa
      241 cttgagacag aatcgaaaaa cgctaaatat tatctggatt taactgcctc cagagtcgct
      301 gaagaagaag caccagagac agccttaact tttttatcaa aaattgggtt aaataaaccg
      361 gctactctta catcctttga gagctatatc aatagctgga taaaaagcaa acaactcgaa
      421 aaggctatgc agcttcccag cctcgatgag aatgcactga attattattt accgactgtg
      481 ttagtcgcat atcagatcgt tcctgataag gcaataagta tttataattc tgtatatgga
      541 gataatgttg ttatgcccta tcagcaagtc accatgttac ttacagttgc ggagggttat
      601 caaacaaaag gcgatataaa aaacgccatt cattatgccg ataaggcgct gaatatgttt
      661 gataaagcta ttgctgtaga tagcagtcgt gaatcccatt atagtgatga gtatttgaaa
      721 ctcatgaata tctacgccac caatggcaac aaagaaaaag cggtggtttt atctcaacgt
      781 ctacacaaag ccattaccga atctcaatcg aattacttaa gtactctgga aggaatttta
      841 ttgttttata gaaataatga tatgcagcaa gactatcaaa acagcctttc aaattacata
      901 gtatttatcg ataaaatttt tgccttttcc ccatcaacaa gaagtgaatt aagcctcatt
      961 aatttattaa attcattaaa tgaggtggaa ttgatgagaa aacttctggc gtttctgatg
     1021 acagcaccag aatacgcttg ttatgatagt cagtattgtt atgaagacaa agtcagcgca
     1081 ttaaaaatcc tgtatcgtca tcatgacaat gagctaatgg acaaatattt cacgactctt
     1141 cttaaagaga tcagtaatca accattagaa gactgggata taataataag taatactggt
     1201 gaaaaattta gtgaagtggg tctaaccaaa tatgctcaac atctcgcatc tttcgctgaa
     1261 gaattttata taaaagagca aaaatcacat tctgaaagtg aaatgagagg actattttca
     1321 tcacttgccg aattatatag ttttggtaat gatactgcca gcgcaaaccg agtgttccat
     1381 cagcatgtcc ctgccttcaa tacagatcat atgattcaat attttataaa tgccaaagaa
     1441 tggaacaatg ccagggaatt attgataaaa gaggaaatgt tgggtatgca taatctgata
     1501 ttgttggaga atatttgtat gcaaaaaaat gcagaatgca tggcacacat tacctttacg
     1561 cttaataaat taacaacaca accagcaata acaaaaatag atagcgtggg taatgagcaa
     1621 ctttatcaga ttggcaaaat ctaccataag ttggaaatta aacctgagcc agagcaacag
     1681 gttttaattc agtcgcttta tgaccgggca tcaggaagcg cctctgcaac tcaataaatg
     1741 catggtcaaa agggccacca cggtggccct gacaatcagt agctatatcc agaaagttaa
     1801 tcgcggaagt ttttgaactg gaacggctga ccgagatcgc caccgcgcac catcgccatg
     1861 acagcctgca aatcatcacg agatttgcca gtcacgcgga tctcatcgcc ctgaatttgc
     1921 gcctgcactt tcagtttgct gtctttgatc atcttgacga tttttttctg agtggcgctc
     1981 tcaatgccct gtttcagttt cgcttccaca aaccaggttt taccgctatg aacgatattt
     2041 tccggtacat ccagtgaact gccttcaatg ccgcgcttca gcaacttggc acgcagaata
     2101 tccagcaact gattgacctg gaagtcggac tcgctcaaca ctttgatggt tttgctggcg
     2161 tcgttcagct caaatgaggc ttcaacgtta cggaagtcaa aacgggactc cacttcgcga
     2221 ctcgcgttat cgaccgcgtt acgtgcttcc tgaagatcaa cttcagagac aatatcgaaa
     2281 gatggcatct tttcctctcc cttcattttt gatgcgaagc ataatacctg caaagttaaa
     2341 taaccaacag tcctgcacca ggacgtatcc cttaattgtg catagtcgca acagcatgca
     2401 caattaaggg atagccttat tgaacacccg gagtggttgc gggtgaggag gaacaatgaa
     2461 aattaccgta ttgggatgcg gtgccttagg gcaattatgg cttacagcac tttgtaaaca
     2521 gggtcatgaa gttcagggct ggctgcgcgt accgcaacct tattgtagtg tgaatctggt
     2581 tgagacagat ggttcgatat ttaacgaatc gctgaccgcc aacgatcccg attttctcgc
     2641 caccagcgat ctgctcctgg tgacgctgaa agcatggcag gtttccgatg ccgtcaaaag
     2701 cctcgcatcc acactgcctg taactacgcc aatactgtta attcacaacg gcatgggcac
     2761 catcgaagaa ttgcaaaaca ttcagcagcc attactgatg ggcaccacca cccatgccgc
     2821 ccgccgcgac ggcaatgtca ttattcatgt ggcaaacgga atcacgcata tcggtccggc
     2881 acggcaacag gatggcgatt acagttatct ggcggatatt ttgcaaaccg tgttgcctga
     2941 cgttgcctgg cataacaata ttcgcgccga gctgtggcgc aagctggccg tcaactgcgt
     3001 gattaatcca ctgactgcca tctggaattg cccgaacggt gaattacgtc atcatccgca
     3061 agaaattatg caaatatgcg aagaagtcgc ggcagtgatc gaacgcgaag ggcatcatac
     3121 ttcagcggaa gatttgcgtg attacgtgat gcaggtgatt gatgccacag cggaaaatat
     3181 ctcgtcgatg ttgcaggata tccgcgcgct gcgccacacc gaaatcgact atatcaatgg
     3241 ttttctctta cgccgcgccc gcgcgcatgg gattgccgta ccggaaaaca cccgcctgtt
     3301 tgaaatggta aaaagaaagg agagtgaata tgagcgcatc ggcactggtt tgcctcgccc
     3361 ctggtagtga agagactgaa gccgtcacca cgatcgacct cttggttcgc ggcggtatca
     3421 aagtcaccac tgccagcgtc gacagcgatg gcaacctggc aattacctgc tcgcgcggcg
     3481 tgaagctgct ggcggatgcg ccgctggtcg aagtggctga tggcgaatat gacgtgatcg
     3541 tgctgcctgg tggcattaaa ggcacggagt gttttcgcga tagcactctg ctggttgaaa
     3601 ccgttaaaca gttccaccgt tccgggcgta tcgtcgcggc tatttgcgcc gcgccagcca
     3661 ccgtgctggt gccgcacgat atctttccga ttggcaatat gaccggcttc ccgacgctga
     3721 aagacaaaat tcccgctgaa caatggcagg acaagcgcgt cgtctgggat gcacgggtaa
     3781 aattgctgac cagccaaggg ccgggtacag ctatcgactt tggtctgaaa attatcgacc
     3841 tgctggttgg gcgcgagaaa gcccatgaag tggcatcaca actggtgatg gcggcaggga
     3901 tttataatta ttacgagtag tgtcggatgc ggcaaacgtc gcatctgacc agatgcgacg
     3961 taaaaactac aaattacggg cgatatacct tcacattgtt aaagccttgc tcgcgcagat
     4021 agagcgcctg cagacggctc atcaccccgc gctcacacca cagcagccag gttttgttct
     4081 ggtcgagatc gccaaatttg gtgctcagtt tatagaacgg cagagaaacc acatcaatcc
     4141 cttcgacttt cagtggctta tcttcctgtt catcgacaga acggatatcg agaatcacgt
     4201 cgttcgggcc gaagccgttg acggtttcca cttccaccac ttcctgctcg gtctgctggg
     4261 cgatttcgcg gatatcaacg ttattcgctt cctcaaccac tttatcgaga atactgaagt
     4321 cgaacttctc ttcttccgct tcaatcttcg atttaaccgc tttcaccgtc gggcttttgg
     4381 agatcacgcc acaatattcc ggcatcgtgc gagcaaagtc ttcggtgcca atctggcgag
     4441 ccaggttgat gatgtgctct ttgtcgtaag aaatcagcgg acgcaggatc agcgtgtcag
     4501 agacgttatc aatcaggcgc aggttggtca gcgtctgact ggacacctgg ccgagcgctt
     4561 cgccggtgac cagcgcctgt acgccgtaac gttcagccac tttagatgcg gcacgcacca
     4621 tcatacgttt gaggataacg cccatctgac cgtcgtcgat tttctcgaga atttccccga
     4681 cgaccggttc gaaattaatg gcgacaaaac gcacgcggtg ggagctgcca aagcggttcc
     4741 acagataatg cgccacctga cgcacgccaa tttcatgcgc tgcgccgccg aggttaaaga
     4801 agcagtaatg cacgcggcag ccgcgacgca tcaacatata actggaaaca ccggagtcga
     4861 aaccaccgga aatgagcgac agcacatctt cctgggtgcc aatcgggaaa ccaccaatac
     4921 cttcgtagcg acctttaatc agcaggaggc gatcgtcttc cacttccaga tgaacagtta
     4981 catccgggtt agtcagcttc acgcgcgcgg attcaatatg ctgatttaaa ccgccgccga
     5041 cgtaacgttc cacatcaatc gagctaaaat catgtttgcc acggcgcttc acgcgtacgc
     5101 agaaggtttt gccttccagc tgatcgcgat actgaaccaa cgctttctca aaaatatcgt
     5161 gcatgtcggt aaacggcacg tcttcgactt cgagaatatg gtggataccc ggaatacggg
     5221 tcagggcgtc gcgaatagtc agacgctggt tttcatcttt cgcgcgaact tcgatgttat
     5281 cccagtggcg gacgacagcg agcgtctcat catagtgctt taaaacgtta cgaatgttcc
     5341 cggtaaggat ttttataaag cgcaagcgca cagattggct tttgatggtg atttccggga
     5401 acaatttaat gataaacttc atggcggcaa tggttcgttg gcaagcctta agcgacttgt
     5461 atagggaaaa atacagcagc ccacacctgc ggctgcatcc aggcgcggaa gtataccact
     5521 aacatcgctt tgctgcgcac atcaccttac cattgcgcgt tatttgctat ttgccctgag
     5581 tccgttacca tgacggggcg aaaaatattg agagtcagac attcattatg ccgaagaaaa
     5641 atgaggcgcc cgccagcttt gaaaaggcgc tgagcgagct ggaacagatt gtaacccgtc
     5701 tggaaagtgg cgacctgccg ctggaagagg cgctgaacga gttcgaacgc ggcgtgcagc
     5761 tggcacgtca ggggcaggcc aaattacaac aagccgaaca gcgcgtacaa attctgctgt
     5821 ctgacaatga agacgcctct ctaacccctt ttacaccgga caatgagtaa tggactttcc
     5881 gcagcaactc gaagcctgcg ttaagcaggc caaccaggcg ctgagccgtt ttatcgcccc
     5941 actgcccttt cagaacactc ccgtggtcga aaccatgcag tatggcgcat tattaggtgg
     6001 taagcgcctg cgacctttcc tggtttatgc caccggtcat atgtttggcg ttagcacaaa
     6061 cacgctggac gcacccgctg ctgccgtaga gtgtatccac gcttactcat taattcatga
     6121 tgatttaccg gcgatggatg atgacgatct gcgtcgcggt ttgccgacct gccatgtgaa
     6181 gtttggcgaa gcaaacgcga ttctcgctgg cgacgcttta caaacgctgg cgttctcgat
     6241 tctaagcgat gccaatatgc cggaagtgtc ggatcgcgac agaatttcga tgatttctga
     6301 actggcgagc gccagcggta ttgccggaat gtgcggtggt caggcattag atttagacgc
     6361 ggaaggcaaa cacgtacctc tggacgcgct tgagcgtatt catcgtcata aaaccggcgc
     6421 attgattcgc gccgccgttc gccttggtgc attaagcgcc ggagataaag ggcgtcgtgc
     6481 tctgccagta ctcgacaagt atgcagagag catcggcctt gccttccagg ttcaggatga
     6541 catcctggat gtggtaggag atactgcaac gttgggaaaa cgccagggtg ccgaccagca
     6601 acttggtaaa agtacctacc ctgcacttct gggtcttgag caagcccgga agaaagcccg
     6661 ggatctgatc gacgatgccc gtcagtcgct gaaacaactg gctgaacagt cactcgatac
     6721 ctcggcactg gaagcgctag cggactacat catccagcgt aataaataaa caataagtat
     6781 taataggccc ctgatgagtt ttgatattgc caaatacccg accctggcac tggtcgactc
     6841 cacccaggag ttacgactgt tgccgaaaga gagtttaccg aaactctgcg acgaactgcg
     6901 ccgctattta ctcgacagcg tgagccgttc cagcgggcac ttcgcctccg ggctgggcac
     6961 ggtcgaactg accgtggcgc tgcactatgt ctacaacacc ccatttgacc aattgatttg
     7021 ggatgtggga catcaggctt atccgcataa aattttgacc ggacgccgcg acaaaatcgg
     7081 caccatccgt cagaaaggcg gtctgcaccc gttcccgtgg cgcggcgaaa gcgaatatga
     7141 cgtattaagc gtcgggcatt catcaacctc catcagtgcc ggaattggta ttgcggttgc
     7201 tgccgagaaa gaaggcaaaa atcgccgcac cgtctgtgtg attggcgacg gcgcgattac
     7261 cgctggcatg gcgtttgaag cgatgaatca cgcgggcgat attcgtcctg atatgctggt
     7321 ggtcctcaac gacaatgaaa tgtcgatttc cgaaaatgtt ggcgcgctta acaaccatct
     7381 ggctcagctg ctttccggta agctttactc ttcactgcgc gaaggcggga aaaaagtttt
     7441 ctctggcgtg ccgcccatta aagagctgct caaacgtacc gaagaacata ttaaaggcat
     7501 ggtggtgcct ggcacgttgt ttgaagagct gggatttaac tacatcggcc cggtggacgg
     7561 tcacgatgtg ctggggctta tcaccacgct aaagaacatg cgcgacctga aaggcccgca
     7621 gttcctgcat atcatgacca aaaaaggtcg tggttatgaa ccggcagaaa aagacccgat
     7681 cactttccac gccgtgccta aatttgatcc ctccagcggt tgtttgccga aaagtagcgg
     7741 cggtttgccg agctattcaa aaatctttgg cgactggttg tgcgaaacgg cagcgaaaga
     7801 caacaagctg atggcgatta ctccggcgat gcgtgaaggt tccggcatgg tcgagttttc
     7861 atgtaaattc ccggatcgtt acttcgacgt ggctattgcc gagcaacacg cagtgacctt
     7921 tgccgccggt ctggcgattg gtggatacaa acccattgtc gcgatctact ccaccttcct
     7981 gcaacgcgcc tatgatcagg tgctgcatga cgtggcaatt caaaagctac cggtcctgtt
     8041 cgccattgac cgcgcgggca ttgttggtgc tgacggtcaa actcaccagg gcgcttttga
     8101 tctctcttac ctgcgctgca taccagaaat ggtcattatg accccgagcg atgaaaacga
     8161 atgtcgccag atgctctata ccggctatca ctataacgat ggcccgtccg cggtgcgcta
     8221 cccgcgcggc aacgcggttg gcgtggaact gacgccgctg gaaaaactac caattggcaa
     8281 aggcattgtg aagcgtcgtg gcgagaaact ggtgatcctt aactttggta cgctgatgcc
     8341 agaagcggcc aaagtcgccg aatcgctgaa cgctacgctg gtcgatatgc gttttgtgaa
     8401 accacttgat gaagcgttaa ttctggaaat ggccgccagc catgaagcgc tggtcaccgt
     8461 agaagaaaac gccattatgg gcggcgcagg tagcggagtg aacgaagtgc tgatggccca
     8521 tcgtaaacca gtacccgtgc tgaacattgg cctgccggac ttctttattc cgcaaggaac
     8581 tcaggaagaa atgcgcgccg aactcggcct cgatgccgcc ggtatggaag ccaaaatcaa
     8641 ggcctggctg gcataatccc tactccactc ctgctatgct taagaaatta ttcatggcag
     8701 gagtggcagc atgcaataca accccttagg aaaaaccgac cttcgcgttt cccgactttg
     8761 cctcggctgt atgacctttg gcgagccaga tcgcggtaat cacgcatgga cactgccgga
     8821 agagagcagc cgtcccataa ttaaacgcgc gctggaaggt ggcataaatt tctttgatac
     8881 cgccaatagc tattccgatg gtagcagcga agagatcgtt ggtcgcgcac tgcgggattt
     8941 cgcccgtcgt gaagacgtag tcgttgccac caaagtgttc catcgcgttg gtgatttacc
     9001 ggaaggatta tcccgtgcac aaattttgcg ctctatcaac gacagcctgc gacgtctcgg
     9061 catggattat gtcgatatcc tgcaaattca tcgctgggat tacaacacgc cgatcgaaga
     9121 gacgctggaa gccctgaacg acgtggtaaa agccgggaaa gcgcgttata tcggcgcgtc
     9181 atccatgcac gcttcgcagt ttgctcaggc gctggaacta caaaaacagc actgctgggc
     9241 gcagtttgtc agtatgcagg atcactataa tctgatttat cgcgaagaag agcgcgagat
     9301 gctgcctctg tgttatcagg agggcgtggc ggtgattccg tggagcccgc tggcgcgggg
     9361 gcgactgacg cgtccatggg gagaaactac cgcacgactg gtgtctgatg aggtggggaa
     9421 aaatctctat aaagaaagcg atgaaaatga cgcgcagatc gcagagcggt taacgggcgt
     9481 cagtgaagaa ctcggtgcaa cacgagcaca agttgcgctg gcctggttgt taagtaaacc
     9541 gggcattgcc gcaccgatta tcggaacttc gcgggaagaa cagcttgatg agctgctgaa
     9601 cgcggtggat atcactttga agccagaaca gattgccgag ctggaaacgc cgtataaacc
     9661 gcatgcggtc gtaggattta aataagagtc caggcctgat gagacgtgac aagcgtcaca
     9721 tcaggcatcg gtgcacaact acgacagaat acccagcggc cagtgatgac cgataaagta
     9781 caggatgcct gcggaaatca ccccggcgac aatatcgtcg atcatgatcc ccatgccgcc
     9841 atgtacattg cgatcaaacc agcggatcgg ccacggcttc cacatatcca gaatacggaa
     9901 aatcacaaac ccggcggcaa cccactgcca gtcattggtc ggcagcgcca tgagcgtgat
     9961 ccacatacca ataaattcgt cccagacaat gctgccgtga tcgtgcacgc ccatatcttt
    10021 cgccgtttga tgacaaagat agacgccgat acagatcccc aacatcacca ccagcgagta
    10081 gagctgccag ggtaaaaagg tcatcagata ccagaaaggg atcgccgcca gcgatcccat
    10141 cgtgccagga acgattgggc ttaatccact tccgaatccg acagcaagca gatgccacgg
    10201 attactcatc ttcaggcgac ttttcgcgac atctttatgg cgtggcaaaa tggtcatatc
    10261 ctttccagtc aaacgtgaca ggttcgccgt cacgaataaa acaaagccct tcgatatcgg
    10321 cggtcatttg cccgatacag gtaaacggta cgccaaggtg tccgagagca acatccagcg
    10381 cgccacggtt cagctccggc accgtgaaac acaactcgta atcttcaccg ccagagagcg
    10441 cccagcgcag cgcctgttcc ggttcaacat ggcgagaaag cgcatcagaa aacggcagca
    10501 atgccaggtc aatacgtgcg ccgcagtcgc tggctttcac gatatgcccg agatcggaaa
    10561 tcagaccgtc agagagatcg atagctgaat ttgccagatc gcgcagtgcc tgcccctgta
    10621 aaatacgcgg cgatggacgg agatgacgtt tgatcaagta gtcagcatct ttagcatcgg
    10681 caacctgcaa acggttttgc aaaatcgcca gcccggcggc gctatcgccc ggtgtaccgg
    10741 tcacatagat ccagtcaccc gatttcgccc cagagcgcgt taaggctcgt cccatcggaa
    10801 caaagccgtg gatacccaac gtcattgata atggcccacg cgtggtatcg ccgccaatga
    10861 gttgcatatc gtaataattg agaagatcaa acaaactgtc gctgaaggac tcaagccacg
    10921 cttcgtctac gtccggtaag gttaatgcca gcgtcagcca ggccggatcg gcccccatcg
    10981 ctgccagatc gcttaggttc accgccagtg ctttataagc cagatcagca ggatcgatat
    11041 cagggaggaa atggttgccc gccaccagcg tatcagtgct gatcgccagg gtctgtttct
    11101 cggggatatt gagaagagcg caatcgtcgc cgatgcccag ttcgacatca agacgagaac
    11161 ttcttacacg gtcaaaataa cgggcaatca gggagaactc gccacatgcc atacgttatg
    11221 cctcagcagg taaaaaagaa aaggccggcg atcatggaac agtcttccgt gaatctaccg
    11281 gcctggatat tactttttgt tagggcgaat cacaggtgct gctttatcga gtacgccgtt
    11341 gacgaactta tggctgtctt ctgcgccgaa cgatttcgcc agttcgatcg cttcgttaat
    11401 ggccactttg tatggcacat cactacgttt agacagttcg tacagcgcaa tgcgcagtac
    11461 tgctttttct acctggccca gttcttccag caggcgggac aggtagggct tcatcagtcc
    11521 gtcgaggtat gcggtgttag tcgccacccc ggccagcagc tcacggaagt acaggacgtc
    11581 aacgtctttt acatcctgtt cagccaggaa ctggtattca acatcagcga tgtcgttctg
    11641 ggacaactgc caggagtaga gcgcctggac ggcacactca cgagcgcggc gacgagcagc
    11701 aggtttcacg gatttcccct tactaatttc aggccttgat ggctttcaat acattaatca
    11761 tttcaagcgc ggtcagtgca gcttctgcac ctttgttgcc agctttggtg ccagcacgtt
    11821 cgatcgcttg ttcaatgctt tcagtggtca gaaccccaaa agcaaccgga atttcgctgt
    11881 cctgggcaac atgctccagg ccgttgcttg caccaccagc gacatattca aagtgggcag
    11941 tgccaccacg aataaccgta cccagcgcaa tcaccgcgtc gtatttaccg gttttagcca
    12001 gtgcacccgc cgccagcggc agctcatagg caccaggcac ccaaacaacg gtaatgtttt
    12061 catcttttac ctgaccgata cgtttcagtg cgtcaattgc accttccagc aggctgtcat
    12121 tgataaagtt gttgaaacgc gcgatggtga tggcgacgcg agcgtccggg gtagcaacgt
    12181 tagcttcaat aatgttcatt ttcttccttc gggttcgagt atggccccgc aggggggcgg
    12241 attttagcat aatatttcgt gcgctgcttc cctttcgagc cgggagatca tgcacccact
    12301 aaatgcaggc aaacatccgg gcctacataa cgtatctctt tgaatttaaa ttggggggcg
    12361 tcggctaatt tctcaagccc tggcagcgag cataatccac gggcgtcgct gcctaatagt
    12421 ttaggtgcga tatagacaat cagctcatcg actaaacccg cctgtagcaa tacgccagcg
    12481 agcgttggcc ccgcttccac ccagatgctg ttaatctgct gtttacccag ttgcatcatc
    12541 agtacaacca gatccagatg acctttatgc tctggaatca gcaaggtacg caccgtttcc
    12601 ggccactcac gagaatcttc ctgcgtacgc gcgaaccagg tttcgccggg ctgctgcaca
    12661 atgcgatgtt ccggcgtcac gcgattttgg ctatcaatca caatacgtac tggctggcgg
    12721 agattttgtt gcgggtagag cgcctgagtt tgttcatcca gttcagacca acgcactgtt
    12781 aaggctggat catccgccag caccgtggcg ctgctggtta aaatggcatg actttgtgcg
    12841 cgctgtcgtt gtacgtcgcg ccgcgcctga ggcgaagtga tccactggct ttcgccgctc
    12901 gccatcgccg tgcggccatc aagcgatgcg ccaagtttta actgaatata aggaaagccg
    12961 gtgcgcatcc gcttgagaaa gcctttattc aattgctcgg cttcactcat catcagcccg
    13021 tggctgacgt caatgccagc ctgttgcaga cggtaaagtc cacgcccagc gacctgcggg
    13081 ttaggatctt gcatcgctgc gaccacgcgc gctacgccag cggcgatgag tgcgtcacag
    13141 cacggtggcg tacggccatg atggctacag ggttcgagtg tcacatacgc cgttgcgcct
    13201 ttggcttttt cacccgccat acgcaacgcg tgtacttcgg catgtggttc acccgctcgt
    13261 tggtggtaac cttcaccgac aatttcgcca tctttgacaa tgacgcaccc gacattcggg
    13321 ttggggtggg tggtaaaacg tcctcgttgc gccagcttta gcgcccgcgc catgtaatac
    13381 tcgtcctgca cggcttagtc ctccaggcgc gcgatctctt cgccaaattc tttgatatct
    13441 tcgaaactgc ggtagacaga ggcaaaacgg atataggcga ctttatcgag ctttttcaat
    13501 tgctccatca ccagattgcc aatcatcttg ctcggtactt cgcgctcacc ggtggcgcgc
    13561 agctgcgatt taatatgatt gattgccatt tcaacgtaat cggaactcac cggacgtttt
    13621 tccagcgccc gcagcattcc gctacgcaat ttctcttcat taaacggttc acgcacgtcg
    13681 ttgcttttta caacacgcgg cataaccagc tccgccactt caaaggtggt gaaacgttca
    13741 ttacacacca gacactgccg acggcggcgt acggatgaac cctcgcccac gagacgagag
    13801 tcaattacct tagtgtccac ggcgaaacag aatgggcaat gcatacggcg tcctgaccag
    13861 gtggttaacg gaaatctatt ttaccctgaa ctgacgatac aacaaaggcg caacgctttt
    13921 gagataaagg atcgatgctt tcgctgcttt taccctctac cattaggcta attgcgacga
    13981 gaaagggacg acctatgaat actaacgttt ttcgactgct cctgctggga agcctgttca
    14041 gccttagcgc ctgtgtgcag caaagtgaag tgcgacagat gaaacacagc gtcagcacgc
    14101 tgaaccagga gatgacgcaa ctcaatcaag aaacagtcaa aatcactcag caaaacaggc
    14161 tgaatgcaaa atccagcagt ggggtttacc ttctgcctgg agcgaaaaca ccggcaagac
    14221 tggaaagcca gatcggtact ttacgtatgt cgctggtgaa tattacgcct gatacagatg
    14281 gcaccaccct tacactacgt attcagggtg agtcgaatga tcccttgccc gcattcagcg
    14341 gaactgttga atatgggcaa attcagggaa caatagacaa ctttcaggaa atcaatgtgc
    14401 agaatcaatt gattaatgcc cctgccagcg tcctcgcccc cagcgatgtt gatattccgt
    14461 tacaattaaa ggggatttct gtggatcagt taggctttgt acgtatccac gacattcaac
    14521 ctgttatgca gtaaacgtat ttcgggacga ttttgtgcgt cccgcaacat cttttcccgt
    14581 cattttgtta ctctgcttac atcacctgga ttgatagtaa aagtttgcaa caagggcgaa
    14641 agtcagtaca atccccgccc gaatgtgtgt aaacgtgaac gcaatcgatt acgtaaatga
    14701 tagaactgtg aaacgaaaca tatttttgtg agcaatgatt tttataatag gctcctctgt
    14761 atacgaaata tttagaaacg caatttgcgc ctttttcact cccgcaaggg attttcaaac
    14821 agtggcatac atatgaaaaa aacattactg gcagccggtg cggtactggc gctctcttcg
    14881 tcttttactg tcaacgcagc tgaaaacgac aaaccgcagt atctttccga ctggtggcac
    14941 cagagcgtta acgttgtcgg aagctatcac acccgtttcg gaccgcagat ccgcaacgat
    15001 acctaccttg agtacgaagc attcgctaaa aaagactggt tcgacttcta tggttatgcg
    15061 gatgcgccgg tattcttcgg cggtaactcc gatgctaaag gtatctggaa ccacggttct
    15121 ccgctgttta tggaaatcga accacgtttc tccatcgaca agctgaccaa tactgacctt
    15181 agcttcggtc cgttcaaaga gtggtacttc gcgaacaact acatttacga catgggtcgt
    15241 aataaagatg gtcgccagag cacctggtac atgggtctgg gtaccgatat cgacactggc
    15301 ctgccgatga gcctgtccat gaacgtctat gcgaaatacc agtggcagaa ctatggcgca
    15361 gcgaacgaaa acgagtggga cggttaccgt ttcaaaatta aatactttgt gccgattacc
    15421 gatctgtggg gcggtcagct gagctacatc ggcttcacca acttcgactg gggttccgat
    15481 ttaggggatg acagcggtaa cgcaatcaac ggtattaaga cccgtactaa taactctatc
    15541 gcttccagcc atattctggc tctgaactac gatcactggc actactctgt cgtagctcgt
    15601 tactggcacg acggtggtca gtggaacgac gatgcagaac tgaacttcgg caacggcaac
    15661 ttcaacgttc gctctaccgg ctggggtggt tacctggtag taggttacaa cttctgatta
    15721 tgaaaatgcc gggatttatt cccggcattt ctgattgtta acaacgtgtg ctttgttcat
    15781 gccggatgcg gcgtaaacgc tttgtccggc ccacaaaagc acgtaaattc aatatattgc
    15841 tgaaataacg taggcctgat aagcgtagcg catcaggcaa ttttacgttt taatcacttc
    15901 ttcttgttca tcatcgcttt caggtcagcg aatgggttgt acttcgcttc accgacatct
    15961 ttctgcgcat cttcccctgc gataacggtc gtaccatact gatccgcttc ggtatatttc
    16021 gaatgctcat gatcgtggca atagagacac aacaattccc agttactgcc atcttccggg
    16081 ttattggtat ggtcgtgatc aatgtggtga acggtaagtt cacgcaggtt agaataaaca
    16141 aactcgcggg aacagcgacc gcagacccac ggatagattt ttaatgcttt ttcgcgatag
    16201 ccgctttcta accgtgcata gttttttggg atgatagcca tgaaaagtta cctggatgaa
    16261 atgtagggtt acgcgcagtt t
//
LOCUS       DADLSA010000087        16183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.087, whole genome shotgun sequence.
ACCESSION   DADLSA010000087 DADLSA010000000
VERSION     DADLSA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.087"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..139
                     /locus_tag="JN691_002708"
     CDS             <1..139
                     /locus_tag="JN691_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HAY7244118.1"
                     /translation="FLMALPPVYLQDLPLTLRPLLSNGVLVGILLAVLMDNLIPWERI
                     E"
     gene            161..1306
                     /gene="glxK"
                     /locus_tag="JN691_002709"
     CDS             161..1306
                     /gene="glxK"
                     /locus_tag="JN691_002709"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HAY7244119.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSPLFPDAHYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(1534..2319)
                     /gene="allE"
                     /locus_tag="JN691_002710"
     CDS             complement(1534..2319)
                     /gene="allE"
                     /locus_tag="JN691_002710"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HAY7244120.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKNGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGTSFVDYLVTLHQNCGNQQCFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(2330..3565)
                     /gene="allC"
                     /locus_tag="JN691_002711"
     CDS             complement(2330..3565)
                     /gene="allC"
                     /locus_tag="JN691_002711"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HAY7244121.1"
                     /translation="MITHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FADAMKACEFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQPLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(3587..4636)
                     /gene="allD"
                     /locus_tag="JN691_002712"
     CDS             complement(3587..4636)
                     /gene="allD"
                     /locus_tag="JN691_002712"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HAY7244122.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLCLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKVAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            4953..6620
                     /locus_tag="JN691_002713"
                     /pseudo
     CDS             4953..6620
                     /locus_tag="JN691_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
     gene            6630..7889
                     /locus_tag="JN691_002714"
     CDS             6630..7889
                     /locus_tag="JN691_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HAY7244123.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPAFSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            7900..8715
                     /locus_tag="JN691_002715"
     CDS             7900..8715
                     /locus_tag="JN691_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HAY7244124.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            8712..9605
                     /locus_tag="JN691_002716"
     CDS             8712..9605
                     /locus_tag="JN691_002716"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY7244125.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9742..10809)
                     /gene="purK"
                     /locus_tag="JN691_002717"
     CDS             complement(9742..10809)
                     /gene="purK"
                     /locus_tag="JN691_002717"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAY7244126.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(10806..11315)
                     /gene="purE"
                     /locus_tag="JN691_002718"
     CDS             complement(10806..11315)
                     /gene="purE"
                     /locus_tag="JN691_002718"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAY7244127.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(11433..12155)
                     /gene="lpxH"
                     /locus_tag="JN691_002719"
     CDS             complement(11433..12155)
                     /gene="lpxH"
                     /locus_tag="JN691_002719"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAY7244128.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(12158..12652)
                     /gene="ppiB"
                     /locus_tag="JN691_002720"
     CDS             complement(12158..12652)
                     /gene="ppiB"
                     /locus_tag="JN691_002720"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAY7244129.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            12826..14211
                     /gene="cysS"
                     /locus_tag="JN691_002721"
     CDS             12826..14211
                     /gene="cysS"
                     /locus_tag="JN691_002721"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAY7244130.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(14247..14768)
                     /locus_tag="JN691_002722"
     CDS             complement(14247..14768)
                     /locus_tag="JN691_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7244131.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(14876..15088)
                     /gene="ybcJ"
                     /locus_tag="JN691_002723"
     CDS             complement(14876..15088)
                     /gene="ybcJ"
                     /locus_tag="JN691_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAY7244132.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(15090..15956)
                     /gene="folD"
                     /locus_tag="JN691_002724"
     CDS             complement(15090..15956)
                     /gene="folD"
                     /locus_tag="JN691_002724"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAY7244133.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAARLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
BASE COUNT         3764 a         4169 c         4492 g         3758 t
ORIGIN      
        1 atttttaatg gcattaccgc ctgtgtattt gcaagacctt ccattaacgc ttcgtcctct
       61 gctcagtaac ggcgtactgg tcggaatttt actggctgtt cttatggata atcttattcc
      121 gtgggaacgc atcgaataat ttgttgaaaa aggattgata atgaagattg tcattgcgcc
      181 agactctttt aaggagagct taagtgcaga aaaatgttgt caggcaatta aagccgggtt
      241 ttcgcccctc tttcccgatg cgcactatat ctgtttaccg atagcggatg gcggcgaagg
      301 gacggtggat gcgatggtcg ccgcgacggg tggcaacatc gtgacgcttg aagtctgcgg
      361 gccgatgggc gaaaaagtga atgcttttta tggccttacc ggagacggga aaacagcggt
      421 gattgagatg gcggcagcaa gtggcctgat gctggtcgcg cctgaaaagc gtaatccgtt
      481 gctggcctcc agttttggta cgggggagtt aattcgccat gcgctggata acggcattcg
      541 ccatattatt ctcggcattg gcggcagtgc gacggtcgac ggcggtatgg gcatggcgca
      601 ggcgcttggt gtgcgtttcc ttgatgccga cggccaggtg ctggcggcta acggcggcaa
      661 tttagcgcgc gtggcaagca ttgagatgga ggaatgcgat ccgcgtctgg cgaacagcca
      721 tattgaagtg gcatgtgacg ttgataaccc gctggtaggg gcacgcggcg cggcggcggt
      781 atttggcccg caaaaagggg caacgccgga gatggtcgaa gagctggaac aggggctgca
      841 aaattacgcc cgtgttttac aacagcaaac tgaaattaat gtctgtcaga tggcgggcgg
      901 cggcgctgcg ggcggtatgg gtattgcggc ggcggtattt ctcaatgcgg atattaaacc
      961 gggcattgag attgtgttga atgcggtcaa tcttgcgcag gcggtgcagg gggcagcact
     1021 ggtgatcacc ggggaagggc gcattgactc gcaaacggca ggcggtaaag cgccgctggg
     1081 cgttgcgtcg gtggcgaagc agtttaatgt accggtgatt gggattgctg gtgtattggg
     1141 tgatggcgtg gaagtggtgc accagtacgg cattgacgcg gtattcagca ttttgcctcg
     1201 tctggcacct ttagccgaag tgctcgccag cggtgaaacc aatctcttca acagcgcgcg
     1261 aaatattgcc tgcgccatta aaataggtca gggaattaaa aactgactct tgcctttaaa
     1321 gcggatgcga tttatttcgt ataagagcgc agtactcatg ccggatgcgg catgagtact
     1381 atatccttcc tgaaaatcgc gcaaattcta tatattgcag aggtcatgta ggcctgataa
     1441 gcgaagcgca tcaggcaatg ttacaaaaaa agccacggta taaaccgtgg caaaatccaa
     1501 catagctaaa aataatcagg cgagtggtat gacttaaatc tctacgtcgc ggttgcaatc
     1561 tttcgagtaa atatagctga acgcttcacc acgccctacg ccataaccag cctgtaaaga
     1621 ataagcgccc ataaagatgt aatcgccttt tttcaccggg atccagttat tgtcgaggtt
     1681 ataaaccccc tgaccggaaa gaatataggc accatgttcc tgaacgtgtg tttcgatata
     1741 accgtggctg gcacctggtg caaaagagag gatatgcatg ttcatatcaa aacctaactc
     1801 tttgggcaga aaatccagca ggatcacatc atccatacct tcatagtgaa tgcgttccag
     1861 ttcgctggca ttgccagaaa ccagccacgg tgcatggcct tctaccggaa catagcggcg
     1921 cttatataaa aagatttggc tgtcttcggc ctgggcgtta acaaacgtca ttaaggagcc
     1981 tggtgggcaa taaagatagc caccttcgct taaggcaaat gttttgcctt cggctttggc
     2041 agtgatattt ccagagatca catacaggaa cgtttcaatg ccttcgccac cgaagcactg
     2101 ttggttgcca cagttttgat gcagtgtgac cagataatca acaaaagagg tacccagctt
     2161 tggcgtggag aggattgtcg cgtcacaatt ttcaaagccc ggaataatat tttttaccag
     2221 accatccggg gttaacagtg cgaaattacc gtttttaaca atcgcacggt tagccagtaa
     2281 atcttcgcgg taaccggtga cgttatttaa atatcccatt tatgactcct tatttctgcc
     2341 aggcaagttg ataaagcatg agtgccaacg ttttgacccc ttcggcaagg tcagtaatat
     2401 tggtgcgttc cgccgggtta tggctgatcc cgttgatgct ggggataaaa atcatgcagg
     2461 ttggtacgcg aggcgcgaaa atttgcgcgt cgtgcccggc accactgtgc atcacccggt
     2521 aattcagttt ttctctttca cacaattctg tcagggtggc gaccagctcc ttattcatcg
     2581 gcacgggttc ttcgtccatc cataaatcga tatcaatacc aatgtccatt tcatcgcaaa
     2641 tcgcccgcat gtcgttttct aacggttggg tgaaatcgcg cagcacggca gcgtcggtat
     2701 gacgacaatc aatggtgaac gtggttttac ccggcaccac atttaccgta ttcgggcgcg
     2761 gctctacttt gccaaaggtc agaaccagcg gatcgcccat ctttttcgct ttttcgatcg
     2821 actgatggca aatgcgactg aaagcgtaaa ccgtatcacg acgataaccc atcggcgtgg
     2881 tgcctgcatg gtttgattcg ccgttcagcg tcaccgtata gcgacgctgc ccgacaattg
     2941 cattcaccac gccaattgat tgcccattac tttccagcac acagccctgt tcaatatgca
     3001 gttcaacaaa ggcttttata tcctgacgcg gagttagtgg ggcgttcggc agagtaaatt
     3061 cgcaagcctt catcgcatcg gcaaaactat ttcctttggc atcacagata ttccgcacgt
     3121 cgtcaggatt cgccagccca aagatatttt tactgcccca gaagacatac gggaagcggc
     3181 tgccttcttc ttctgccatc gccaccactt cgaccgtacg taacggtgcg ccgtattgcg
     3241 ttttcaacca gtcaattgcc agccacgccg ccagcgcgcc gaattgcccg tcgaggttac
     3301 cgccgttaac cacggtatcg atatgcgaac cgctcagaac cacttcctgt ggatattcgg
     3361 tgccactcag gcgaccgtat aaattcccca cttcatcgaa acgtgtttcc agcccacttg
     3421 ctgccattct ttttttaaat tgctgctggg tttccagcca ttccggcgaa taaagtaaac
     3481 gggtcatccc acccgttggg tcagcgccaa aagaggaaag ccagggcagc gtttcttcta
     3541 tagcttgacg ggaatgtgta atcataagaa agtcctgtct caataattat tgcgcaaagg
     3601 gatttttcgt ttcgtatgac gtgttataaa gcgcgtcgga aattaaatac tggtaaatat
     3661 catcaacaat ttcgatgcct tcgacggcga ctttgcgttg tttaatatcc tgatcctgtc
     3721 cgggataata aacctgatta aaaccgggcg cgggggtaat ggcatttaat tcgcgcatgg
     3781 tctggctaag atgttgacgg aataattcgc tggaggagaa aaagttcgga ttaataacta
     3841 tatgtaattg ccccaaatta cgccctgcgt gtaaatcgtc atacatcgaa ctaacctgtc
     3901 gcccgaacgg taagcagagt aagacgcctg agaggacgtc aatcatcatc atcaggccat
     3961 acccttttgg cccagcggcg gggagcagag catgtaccgc gaacggatcg gttgttggtg
     4021 caccgttttt atcgaccgcc caggtatccg ggatagacat attacgcgag cgggcgtcga
     4081 gcacttttcc ccatgcctgt acggtagtcg ccatatcaaa ggtaaggatc tcgtcgcctt
     4141 ctcccggtgc ggcaaaggcc agtgggttag taccgtagta aatttccgcg ccgccaaacg
     4201 gcaccaccat tggatcggac tggcacatcg aaatgccaat taatccggcg cgggctgcct
     4261 gctgcacaaa ataagagatt gcgccgctgt gacccatccg gctgataccg accaccgcaa
     4321 cgcctttttg ctgggcggtt ttgatggcat gttccatacc cattttcgcc gcgacctgcc
     4381 cggcggcatt gtcagcatgt aaaattgccg agcacggccc ggtttcctca agacgaaact
     4441 ccggttcgcg gttggtgccg ccttttgaaa tgcgttccgc gtagtattcc acgcgcaccg
     4501 cgccatgaga gtggatccct ctggcatcgg cgtaaaccaa tacttcagcc acggttgcag
     4561 cgtgctcacg ttttaaccca gcctggcaga gtttattctc gattagctgg tggagtgttt
     4621 cccgactaat tttcatctgt cttccttttt aacgacggtg tgaagcatga ctgcaattaa
     4681 catacaggga aaatatctgg attatgtgat ccagacaggc aaaaaaatat agttagaatt
     4741 tatttgataa tccgctcgct tttaacctgg tttttaaaac aaccacgctt attaaaaaat
     4801 aacacgtaat agaatggcgg ttatttgaat tcttttatta ataatccttg tgtgatattc
     4861 atcaccttat ttacccgttg tcatcgatac cgtaatcgcc acattaacac tgctcgtgca
     4921 attgccatgg gtgcaatttt taaggagttg ttatgatcca cgcctttatt aaaaaagggt
     4981 gttttcagga ttcggtcagt ttaatgatta tttcacgaaa actcagcgaa tcagaaaatg
     5041 ttgatgatgt ttccgtaatg atgggtacgc ccgccaataa agcgttatta gataccacag
     5101 gtttctggca tgacgatttt aataacgcca cgccgaacga tatttgcgtg gcaattcgta
     5161 gcgaagcggc ggatgcgggg atcgcgcagg cgattatgca gcagcttgaa gaggcgctaa
     5221 aacaactggc gcagggttca ggcagcagcc aggcgttgac gcaggtgcgt cgctgggaca
     5281 gcgcctgtca gaaattaccc gatgccagtc tggcgctgat ttcagtggct ggcgagtatg
     5341 cggcggagct ggcgaaccag gcgctggatc gcaacctcaa cgtgatgatg ttctccgata
     5401 acgtcacgct ggaagatgaa atccaactca aaacccgcgc gcgggaaaaa ggcttgctgg
     5461 tgatggggcc ggactgcggt acatcgatga ttgccgccac gccgctggct tttgctaacg
     5521 tgatgccgga aggcaacatt ggcgtcattg gcgcttccgg taccgggatt caggagctgt
     5581 gctcacagat tgcgctggca ggggagggaa ttactcacgc gattggcctt ggcgggcgcg
     5641 acctcagccg tgaagtgggc ggcatcagtg cgctgacggc gctggaaatg ctcagtgcag
     5701 acgagaaaag cgaagtgctg gcatttgttt caaaaccacc tgccgaagct gtgcgtctga
     5761 aaattgttaa tgccatgaaa gcaaccggca aaccgacggt ggcgctgttt ttaggttata
     5821 ccccggcggt ggcacgcgac gagaatgtct ggtttgcctc ctcgctggat gaggccgcac
     5881 gcctggcttg cctgctttca cgcgttacgg cgtgacgtaa cgcgataacg cctgccagca
     5941 gcggatttat ttgcggtttg tataccggcg gtacgctggc tgccgaagcg gcgggattac
     6001 ttgccggata ccttggcgtg gaagtcgatg atgcccatca tcacggcatg atgctcgacg
     6061 ccgatggtca ccagattatt gacctgggcg atgatttcta cacagtcggg cgtccccatc
     6121 cgatgatcga cccaacctta cgcaaccagt taattgccga tctcggcgct aaaccacaag
     6181 tgcgcgtatt gctacttgat gtcgtgattg gcttcggtgc gactgccgat cctgccgcct
     6241 cgctggtaag cgcctggcaa aaagcctgtg ccgcgcgttc agataatcaa ccactgtatg
     6301 ccattgccac ggtgacaggc actgaacgtg acccgcaatg ccgctcgcag caaatcgcca
     6361 cgctggaaga tgcggggatc gcggtagtga gttcgctgcc ggaagccacc ttgctggcgg
     6421 cagcgttaat ttatccgctc tcgcctgccg cacagcaaca tacaccgtca ttactggaaa
     6481 acgtcgccgt gattaacatc ggattacgca gctttgcgct ggagctacaa agcgccagca
     6541 aaccggttgt gcattaccaa tggtcgccag tcgccggcgg caataaaaaa ctggctcgtt
     6601 tattagaacg tttgcaataa ggggttccca tgtttacatc agtggcgcaa gccaatgctg
     6661 cggttatcga acaaattcgt cgtgctcgtc cacactggct ggatgtgcaa ccggcttttt
     6721 cacttatcag cgaactaaac gagggcaaaa cactgcttca cgccgggccg ccaatgcgct
     6781 ggcaggagat gaccggaccc atgaaagggg cgtgcgtggg cgcatgtctg ttcgaaggct
     6841 gggcgaaaga tgaagcgcag gcactggcga tgctggagca gggggaagtg aacttcattc
     6901 cttgtcacca tgtgaatgcc gtcgggccaa tgggcggcat tacttctgcc agtatgccga
     6961 tgctggtggt cgagaacgtg accgacggca accgggcgta ctgcaacctc aacgaaggta
     7021 tcggcaaagt gatgcgtttt ggcgcttacg gcgaagatgt cctgactcgc catcgctgga
     7081 tgcgcgatgt gttaatgcca gtattaagcg cggcgctggg gcgcatggag cgcggtatcg
     7141 atctcacggc gatgatggcg cagggcatta cgatgggcga tgagttccat caacgcaata
     7201 ttgcttcctc agcactgtta atgcgtacgc tggccccaca aattgctcgc ctcgatcatg
     7261 ataaacagca cattgcggaa gtgatggatt tcctcagcgt gaccgatcag ttcttcctca
     7321 acctcgcgat ggcctactgc aaggcggcga tggatgctgg cgcgatgatc cgcgcaggca
     7381 gcatcgttac ggctatgacc cgcaacggca atatgttcgg gattcgggta agcgggctgg
     7441 gtgaacgctg gtttactgcg cctgtaaaca ctccgcaagg tctgtttttc accggcttct
     7501 cgcaggagca ggcgaacccg gatatgggcg atagcgcgat taccgaaacc tttggtatcg
     7561 gaggtgcggc aatgatcgca gcgcctggcg taacgcgctt tgtcggtgcg ggtggcatgg
     7621 aagcggccag ggcggtatct gaagagatgg cggaaattta ccttgaacgc aatatgcagt
     7681 tgcagatccc cggctgggat tttcagggcg cgtgcctggg gctggatatt cgtcgcgtgg
     7741 tagaaaccgg cattacgcca ctcatcaata ccggtatcgc ccataaagag gcggggatcg
     7801 ggcagattgg cgcaggcacc gtgcgggcac cgctggcgtg ctttgaacag gcgctggaag
     7861 cactggctga aagcatgggg attggttgag gaacgcgcaa tgacgatcat ccatcctctg
     7921 cttgccagca gtagcgcacc gaattatcgc cagtcctggc ggttagcggg agtgtggcgg
     7981 cgggcgatta acctgatgac ggaaagcggc gaactgttaa cgctgcatcg tcagggtagt
     8041 ggtttcggcc ccggaggatg ggtgcttcgc cgcgcgcaat tcgatgcgtt atgcggtgga
     8101 ttatgcggca atgaacgacc acaggttgtg gctcaaggga ttcgcctcgg gcgtttcacg
     8161 gttaaacagc cacagcgtta ttgtttgctg cgtattacgc cgccttcgca tcctcaaccg
     8221 cttgcagctg catggatgca acgcgcggag gaaaccggac ttttcgggcc actggcgatg
     8281 gcggcaagcg atccgctgcc cgctgaatta cgccagtttc gtcactgttt tcaggccgcg
     8341 ctcaatggcg ttaagaccga ctggcggcac tggctgggta aaggccccgg attaacgcca
     8401 agtcatgatg acacgttgag cggaatgctg ctggcggcct ggtattatgg cgctttagat
     8461 gcgcgcgccg gtcgcccgtt ttttgcctgt tccgacaatc ttcaactcgt taccacagcg
     8521 gtgagcgtca gttatttacg ttatgccgcg caaggatatt tcgcctcgcc actcctgcac
     8581 tttgtccatg ctctgagttg cccgaaacgt accgctgttg cgattgattc gctgctggcg
     8641 ctggggcata cgtcaggggc agatacgctg ctggggttct ggcttggcca acaattatta
     8701 caaggaaaac catgaaaaca ctggttgtag ctcttggggg caacgcctta ctccagcgcg
     8761 gtgaggcgct gacggcggaa aatcaatatc gcaatatcgc cagtgctgta cccgcgctgg
     8821 cacgcctggc ccgttcttat cggctggcga ttgttcacgg caacgggccg caggtggggc
     8881 tgctggcgtt acagaatctg gcgtggaaag aggtagaacc gtatccgctg gatgtgctgg
     8941 tggcggaaag ccaggggatg attggctata tgctggcgca gagcttgagc gcacagccgc
     9001 agatgccgcc tgttacgacg gtgctgacgc gcattgaggt ttcgcctgat gatccggcgt
     9061 ttttgcagcc agagaaattt attggtccgg tttatcagcc agaagaacaa gaggcactgg
     9121 aagcggctta cggctggcag atgaaacgtg atggtaaata tttgcgccgg gtggtggcgt
     9181 ctccgcaacc gcgtaaaatt ctcgacagcg aagccatcga gttgttgctc aaagaggggc
     9241 atgtggtgat ttgcagtggc ggcggcggtg tgcctgtgac ggaagatggg gcagggagtg
     9301 aagcggtgat tgataaagat ctcgccgctg cgttgctcgc cgagcagatt aatgccgatg
     9361 gactggtgat cctcaccgat gctgatgcgg tatatgaaaa ctggggaacg ccgcagcaac
     9421 gtgccattcg ccatgccaca ccggatgagt tagcgccatt tgccaaagcc gatggttcga
     9481 tggggccgaa ggtaacggcg gtgagtggtt atgtcagaag ccgtagtaaa cccgcgtgga
     9541 ttggggcgtt atcgcgaatt gaagagacgc tggcgggcga agcggggacc tgtatttcgc
     9601 tgtagtgatg agagcatccg gcaatcggtg caccatgcct gatgcgacgc ttgacgcgtc
     9661 ttatcaggcc tacaatgggt accggagcgg taagccggat aaggcgttta cgccgcatcc
     9721 ggcaatcaaa tgagcacctg cttaactgaa cttactctgc gcccaaatca cgccgctggc
     9781 atactctggc ggcagcagcg ggattaaggc ttccagcgtc gcagtcagac gcgatgtgtc
     9841 gctgtcggtc aaattcagat gccccacttt acgccccgga cggacttctt tgtcgtacca
     9901 gtgcagatgc accagcggca atttcagcca gtcataattc acatcgctac caatcagatt
     9961 gatcatcacc gacggattat tcactactgg ttgcggtaac ggcagatcgg taatcgcccg
    10021 cagatgcagc tcaaactggc tgatgctggc accgttttgt gtccagtgac cgctgttatg
    10081 cacacgcgga gccagttcgt tgatcagcag accttgcggg gtgacaaaac actccatcgc
    10141 catcacgccc acatagccca gctcctgcat aatcgccgac agcatctctt cggcttgcgc
    10201 ctgctgctgt gcgttggcct gcggaaaagc gacgctggtg cgcaaaatac cgtcctgatg
    10261 caggttatgc gtcagcggat aaaacacggt gctgccatca aagccgcgcg cgccaaccag
    10321 cgatacttca ccagagaagt taatgccctg ctcgacaata cattcaccgt aacactctgc
    10381 cggtaactgt tcggtttcat tggcgcgtaa actccattga ccgcgaccgt catagccacc
    10441 agtgcgacgc ttaacaatcg ccagttcacc taaacgatca aacaccgcag gccactcgct
    10501 gcgatcggca agtaactgcc acggcgcggt cggcaggtgg agcttatcga aaagctgttt
    10561 ctgagtcaga cggtcagcaa taatcgggaa cacatcgcgg ttcacaaagg ccggatgacg
    10621 cgccagctcg cgggttaatg cggtttccgg ccagcgttcg atctcagcgg taatcacgct
    10681 ttgttgaaaa ggcaccgccg ccggttcagc gtccagcccg accggccaga cagcaatgcc
    10741 taacggttcg cctgcctgac gcagcatacg gcctaactgc ccgttaccga ggacgcaaac
    10801 ctgtttcatg ccgcacctcg cgggtccggg ttttccagca cttcgtcggt ctgggctttg
    10861 cgccagtcat tcagacgctg gtgtagctct ttatcgtgag tcgcgagaat ttgtgctgcc
    10921 agtaatgccg cgtttgccgc gccagcttta ccaatcgcca gcgtacccac cggaatgccg
    10981 cgcggcattt gtacgatgga gtagaggcta tcgacaccgc tcagtgcggc gctctgtact
    11041 ggcacgccca gcaccggcac cagcgttttg gcggcaatca tgcctggcag gtgcgctgcg
    11101 ccgcctgcgc ccgcaataat cacctgataa ccgttctctt cggcgctttc ggcgaagctg
    11161 aacagtttat cgggggtgcg gtgagcagaa accacttcaa cgtggtgcgg gacattcagg
    11221 atttcgaaga tttcgacggc gaactgcatg gtagcccagt cgcttttgga ccccatcacg
    11281 atggcgacac gcgccggatt attgcgggaa gacatgcgtc ttaaaactcc tgtggtgcac
    11341 aactctcggc tttagagggc acagagaata gcacggaaag agagcaagga aaacggttgc
    11401 gtggctgtga aatcagcaaa gttgtgggtt ttttaaaacg gaaaatgaat cagctcaacg
    11461 tcatccgccg tgactttcac catcgaacct tccgtatgcc aggcacccag taccacgcga
    11521 aaagcaggtt gctggttggc gataagttca tgcaccgccg ggcgatgggt atgcccgtgg
    11581 atcagccatt gcacctgatg tttttccatc gcactgacca cagcgttttg gttaacgtcc
    11641 atgatcgcca gcgatttact gctgttggct tctttgctgt tcgcgcgcat tcgcgcggca
    11701 atgcgtttgc gcacaaacaa cggcagggcg aggaatagca tttgcagcca gggtttgtgg
    11761 actttggcgc gaaaagcctg ataacccgcg tcatcggtgc acagcgtgtc gccatgcata
    11821 atcaacaccc ggcgaccata aagttcgagc accttttctt ccggcaataa tgtcatgccg
    11881 ctttcacggg caaagcgttt gccgagcaga aaatcacggt tgccatgaat gaaataacag
    11941 ggaacgccag aatcggacac cgctttgatc gctgccgcca tctggcgatg gagtgggttg
    12001 ggatcgtcgt cgccaatcca tgcttcaaac agatcgccaa gaatatacag cgcgtcggcc
    12061 ttgcgggctt cccccgctaa aaaacgcaga aaaccggcgg tgatcgccgg ttcttccacg
    12121 cagagatgaa gatctgcaat aaagagtgtc gccacgatta ctcgctaacg gtcacgcttt
    12181 caatgataac gtcttctttt ggcacgtcct ggtgcatacc gttacgaccg gttgcaacac
    12241 ctttgatttt gtctaccacg tccatgccgt caaccacttc agcaaacacg cagtagcccc
    12301 aaccttgcag gctttcgcca gagaagttca ggaagtcgtt atcaaccacg ttgatgaaga
    12361 actgtgcagt tgcagagtgc ggagcctgag tacgtgccat tgccagcgta ccacgggtat
    12421 ttttcaggcc gttgttggct tcgtttttga tcggttcttt ggtggctttt tgtttcatgc
    12481 ccggttcaaa accgccgccc tgaatcataa agccgttgat aacacggtgg aaaatggtgt
    12541 tgttgtaaaa accttcgcgg cagtagtcca ggaagttttt aactgtttca ggtgctttat
    12601 cgtcaaaagt tttgatgaca atatcgccgt gattggtgtg gaaagtaacc atttttgcat
    12661 cctgttccgt ttgattggtg cttcaaccca gttcgggtca tatatagggt ggtgttatag
    12721 cataaccgca cgatcggatc atcacgcaat gtatgctgat tcgcgcggga aatatgggta
    12781 ttatacgcaa ctcaattacc cacacatgtc taaacggaat cttcgatgct aaaaatcttc
    12841 aatactctga cacgccaaaa agaggaattt aagcctattc acgccgggga agtcggcatg
    12901 tacgtgtgtg gaatcaccgt ttacgatctc tgtcatatcg gtcacgggcg tacctttgtt
    12961 gcttttgacg tggttgcgcg ctatctgcgt ttcctcggct ataaactgaa gtatgtgcgc
    13021 aacattaccg atatcgacga caaaatcatc aaacgcgcca atgaaaatgg cgaaagcttt
    13081 gtggcgctgg tggatcgcat gatcgccgaa atgcacaaag attttgatgc tttgaacatt
    13141 ctgcgcccgg atatggagcc gcgcgcgacg caccatatcg cagaaattat tgaactcact
    13201 gaacaactga tcgccaaagg tcacgcttat gtggcggaca acggcgacgt gatgttcgac
    13261 gtcccgaccg atccaactta tggcgtgctg tcgcgtcagg atctcgacca gctgcaggct
    13321 ggcgcgcgcg ttgacgtggt tgatgacaaa cgcaacccga tggacttcgt tctgtggaag
    13381 atgtcgaaag agggcgaacc gagctggccg tctccgtggg gcgcgggtcg tcctggctgg
    13441 cacattgaat gttcggccat gaactgcaag cagctgggta accactttga tatccacggc
    13501 ggcggttcag acctgatgtt cccgcaccac gagaacgaaa tcgcgcagtc cacctgtgcc
    13561 catgatggtc agtatgtgaa ctactggatg cactccggaa tggtgatggt tgaccgcgag
    13621 aagatgtcca aatcgctggg taacttcttt accgtgcgcg atgtgctgaa atactacgac
    13681 gcggaaaccg tgcgttactt cctgatgtcg ggccactatc gcagccagct gaactacagc
    13741 gaagagaacc tgaagcaggc gcgtgcggcg ctggagcgcc tctacactgc gctgcgcggc
    13801 acagataaaa ccgttgcgcc tgccggtggc gaagcgtttg aagcgcgctt tattgaagcg
    13861 atggacgacg atttcaacac cccggaagcc tattccgtgc tgtttgatat ggcgcgtgaa
    13921 gtgaaccgtc tgaaagcaga agatatggca gcggcgaatg caatggcatc tcacctgcgt
    13981 aaactttccg ctgtattggg cctgctggag caagaaccgg aagcgttcct gcaaagcggc
    14041 gcgcaggcag acgacagcga agtggctgag attgaagcgt taattcaaca gcgtctggat
    14101 gcccgtaaag cgaaagactg ggcggcggca gatgcggcac gtgaccgtct taacgagatg
    14161 gggatcgtgc tggaagatgg cccgcaaggg accacctggc gtcgtaagta attgcgcaat
    14221 tgccggatgc gagttttcgc atccggttat cgtctgcgcc accacaacat tcccatcagt
    14281 agcatccccg gcaaccacac ccacatcaat tcggaaataa tcacctgatg cccgtacgct
    14341 gtggtgtaac gagacaatgc aaacggcgcg actttaatca cctgccaggg agcgaaaaag
    14401 cgttcatctg accacggcca cagccagcca acgcccttac cgccagtggt taccgaatcc
    14461 agtaagctgt gcgatagcaa cgagacggtt aaaaacagcc agcagcgaat cagcccagcc
    14521 ctgaaccatc gacgtccaat cagcacacat aagagcggga caacaaacgc aaacaccagc
    14581 gaatgggtaa acccgcgatg accaaaaaca ttgccgtaag caacgccaaa tttaaacgac
    14641 aatacgtcgg catcgggcag catcgccagg atgattccgg caaataacag acgcggaggg
    14701 atgactttcg aacccaaccc taaaccaatg caaaggggaa cggcggcgtg cgtaataacg
    14761 gttggcatga tggtcgcttc ggcaaaatgt cgatgctatc agcatggatg aacggggcgt
    14821 agagggcaaa agtctgaaaa gagaaccggc ctgttgatac aggccgggaa agggatcagg
    14881 caacaacctg tacgctgtgt cctgcaaaac tcactgtctg accggcgacg attttgcagc
    14941 gtttgcgcgt ttcaaccgca ccgtcgactt tcacctggcc ttcggcaatc gcgattttcg
    15001 cctgcgcgcc gctttcgctc cagccttcca gtttcagcaa gtcgcacagc tcaacgtgcg
    15061 gatgtttacc taaagaaaat gtcgccatgt tactcaccct gtggatcatg atattcaacg
    15121 cacgcctgta gcgtgttttc aatcagcgtg gcaaccgtca tcgggccaac gccgccggga
    15181 acaggcgtaa tgtatgaggc gcgtttagcc gcgtcttcaa acacgacgtc gcccacaact
    15241 ttgccatttt ccagacggtt gatgccgaca tcaatcacaa ttgcgccttc tttgatccag
    15301 tcaccgggaa taaagcctgg cttgccaacg gcaacgatca acagatcggc attttctacg
    15361 tgatgacgca gatttttagt gaagcggtga gtcacggtgg tggtgcaacc tgcgagcaac
    15421 agttccatgc tcattgggcg accgacgata ttcgatgcgc caatcaccac ggcgttgagg
    15481 ccgaaggtat caatgttgta acgctcaagc agcgtgacga taccgcgcgg ggtgcaggga
    15541 cgcagacgcg gcgcgcgctg gcacagacga ccgacgttgt aaggatggaa accgtccacg
    15601 tctttgtccg gatgaatacg ttccagcact ttgacgttat caatacccgc cggtaacggc
    15661 agttgaacca gaatgccatc gatggtgttg tcggcattca gcgcatcgat aagctccagc
    15721 agctccgctt cgctggtggt ttccgggagg tcataagagc gggagacgaa cccgacttct
    15781 tcacaagcct tgcgtttgct tgcgacataa atttgcgatg cagggttact acccaccagc
    15841 acaacggcca gtcctggtgc ccgcagtctg gctgcaatac gagcctgaac tttttgagca
    15901 acttcagagc gcacctgctg cgcaatcgtt ttaccgtcaa taatctttgc tgccatcaga
    15961 gagagaattc catctgttac gtaggtcgaa ggggatgcgt ctattttgtc agaagcgggg
    16021 cgcgctgtca ggtttcgttt cagatttatc gcgtgaagcg acctcttgcg aaggttaggc
    16081 gcaccgtcgc tgaggtcgct gagattgaaa gcttcatttt tcgtacatga tggcgttgta
    16141 aatctggaac tgatttattt ccttgtttaa ggattaagat aat
//
LOCUS       DADLSA010000088        16110 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.088, whole genome shotgun sequence.
ACCESSION   DADLSA010000088 DADLSA010000000
VERSION     DADLSA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.088"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..251)
                     /locus_tag="JN691_002725"
     CDS             complement(<1..251)
                     /locus_tag="JN691_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HAY7244134.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLTACRAALDAGCAAAMRTGRVISRKEIGRPDDDT"
     gene            complement(264..923)
                     /gene="eutL"
                     /locus_tag="JN691_002726"
     CDS             complement(264..923)
                     /gene="eutL"
                     /locus_tag="JN691_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAY7244135.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPQHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(933..1820)
                     /locus_tag="JN691_002727"
     CDS             complement(933..1820)
                     /locus_tag="JN691_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HAY7244136.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(1841..3202)
                     /locus_tag="JN691_002728"
     CDS             complement(1841..3202)
                     /locus_tag="JN691_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HAY7244137.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSSVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            3390..4585
                     /locus_tag="JN691_002729"
                     /pseudo
     CDS             3390..4585
                     /locus_tag="JN691_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416937.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            4831..5418
                     /locus_tag="JN691_002730"
     CDS             4831..5418
                     /locus_tag="JN691_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244138.1"
                     /translation="MNEIKKPGKAATSSGRYEKQAKGEFLMSDMLAVEQETNNDVRQY
                     LNKINELRNKAPKNEETKHEEHTPDHYEAKQQEQAWRGNLRYLDTLNRLDEVLPRKLY
                     ERWEKEHTVNDEAVLRALCYFAGTGKNSQLGWCRVGRGTIDKRARLSKNTVKKCLDRL
                     VHHFKLVERTEGYIPGSAERECNEYQLLFKPYNMK"
     gene            6868..7059
                     /locus_tag="JN691_002731"
     CDS             6868..7059
                     /locus_tag="JN691_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7244139.1"
                     /translation="MNDSELMTVNEVAALFGVTRRTIFRWMNKIKGWPVPVSPIDSRI
                     KFIRSEILEFYKNKGARHQ"
     gene            7199..7462
                     /locus_tag="JN691_002732"
     CDS             7199..7462
                     /locus_tag="JN691_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244140.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFQEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKARLLFELSRRFGVSISTFK"
     gene            7480..8022
                     /locus_tag="JN691_002733"
     CDS             7480..8022
                     /locus_tag="JN691_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244141.1"
                     /translation="MDAFDVLTNKGDELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIEIIKPQIPQIVADAIKAAGLEKRIAGLKTNVEKMEMHKQSGAPTPDEYFSPD
                     AEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     AADPEAEQRKRVFNAIGIRY"
     gene            8119..8448
                     /locus_tag="JN691_002734"
     CDS             8119..8448
                     /locus_tag="JN691_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244142.1"
                     /translation="MFGNYISTSPEKIIMLALRIMQGIAKTLAEHVLDLKHSPLSKQA
                     MKRQTLRLWAEYSLGTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGID
                     INDGTGD"
     gene            8487..8693
                     /locus_tag="JN691_002735"
     CDS             8487..8693
                     /locus_tag="JN691_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244143.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            9152..9490
                     /locus_tag="JN691_002736"
     CDS             9152..9490
                     /locus_tag="JN691_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7244144.1"
                     /translation="MLDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMA
                     EPEGRCVRMLSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEP
                     DLRSCANMLA"
     gene            9471..9749
                     /locus_tag="JN691_002737"
     CDS             9471..9749
                     /locus_tag="JN691_002737"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244145.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDEKTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            9742..11202
                     /locus_tag="JN691_002738"
     CDS             9742..11202
                     /locus_tag="JN691_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7244146.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            11224..12045
                     /gene="murI"
                     /locus_tag="JN691_002739"
     CDS             11224..12045
                     /gene="murI"
                     /locus_tag="JN691_002739"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY7244147.1"
                     /translation="MRLNMFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNM
                     NAPYGNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPI
                     LSIIEPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLI
                     EEGNLNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAH
                     DQAISIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(12164..12858)
                     /locus_tag="JN691_002740"
     CDS             complement(join(12164..12612,12612..12858))
                     /locus_tag="JN691_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7244148.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(13382..14785)
                     /gene="eutA"
                     /locus_tag="JN691_002741"
     CDS             complement(13382..14785)
                     /gene="eutA"
                     /locus_tag="JN691_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAY7244149.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGVAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(14782..16008)
                     /gene="eutH"
                     /locus_tag="JN691_002742"
     CDS             complement(14782..16008)
                     /gene="eutH"
                     /locus_tag="JN691_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HAY7244150.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMR
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
BASE COUNT         4317 a         3926 c         3874 g         3993 t
ORIGIN      
        1 tgggtgtcat cgtctggccg accgatctcc ttacggctga tgacacgccc ggtacgcatc
       61 gcggcagcac aacctgcgtc cagcgccgca cgacacgccg tcagatcgcc ttccaccacc
      121 agcgttaagc gaccagggtc aagcacttcg tgactgagca gacgaacgtt agctgctttg
      181 agcatggcat ccgctgcatc tatcgcggcg accatgccgt ccacttccag caatcccagt
      241 gcattgatca tcggcaacct ccgttacgca cgctggattg gattacgcgc gatttccagc
      301 actgcatcgg taaaggcgtt acaggctgct ttacacgcgg cctggctacc ggttaaaaat
      361 gctgccgagt agttggtttc agacggtggc gggacgtagg tcaccagctg tacgtcggca
      421 gatttcaacg cggcatcaat gccgtaggtc gcttccagcg gcggtgccac cagatacgcc
      481 atcggatcgc caagcgtgat cccggcggtt gatgaaagat aagaaccggt acgcgaaacc
      541 acatgcgcca ggaaggcggt gttttctgcg tcattggccc actggaaagc tgcgccattt
      601 tcaatatgtg caaccatcgc atccagaccg gcacgcactt ccgccgggtt tggcccacca
      661 agcataatca gcacttcacc ggcagtcggt gacgggccgt gtgccgctcc cgcatacagc
      721 gagcgaccat ataccacttc gaccatcgct tgtttggtcg cttcgtcagc cgcaatgtag
      781 gttacgtcgt cagaatcggc agaaatcagc ccgaggctac gaatatgctg cggcaatttt
      841 agttcacgcg caaattcagc gttaacggag gcaatcaccc gcatggcggt taccgacggt
      901 cgaatcaaat ctaaagctgg catgatgcct ccttatcggg tcatgttgat gccggatgct
      961 ttctgctcca gcatacgttt cgccaaatcc acaatgacgg ctgcggcttc aaccggcggc
     1021 gtgccgccct ggtgaatgtt agagatacag gtgcgatcgg cctcaacggt ggtcgccata
     1081 cgcggcgagt aaacggcgta gcaggagagg ctttctgact gccctaaccc cggacgttcc
     1141 cccaccagca ggatcaccac tttcgcgccg aggatctcac cgatctgatc ttcaatcttc
     1201 acgcgaccat aacgaacaaa gaatggcgtg ccgactttca gcccggcctg tttcaggccc
     1261 gccatcagcg gcggcaggat ctcttcgtag ttgacggtga tggcatcagt tgacaggcca
     1321 tcagaaataa cgacctgtac gtccggatta gcaacacact gtgctttcag cgcttcaaca
     1381 gcttctgcac acaggcggcg gcccatatcc gggcgagtca ggtagagatt tttgtcgcta
     1441 atttcagagc gtacttccag caagccctgc gctttcaccc actcttccgg cacttctttc
     1501 agtacggtat ctttcgaacg agagtgatcg gccaggaaac gcagcagcgc ctgagtacgt
     1561 ggacgcggac cggcacgacc ggtacaaacg cgggccacgg tgctgcggcg cagttctgtt
     1621 aatacgtctg cgcgatgcgg gttttcaaca ccaatccacg cttttgcttc agcggaacct
     1681 aaatccagcg cacagctttc cgaggtcacc ggtgccgcac agttggtggt ggcgcacttt
     1741 gcttctgacg gcgcgggggc cgcttgtccc attgacgcca tcacgctgcg tacaatttct
     1801 tcaatctgtt tttgatccat gatgtgttat ctccgcgtca tcagaagaac agtgacggat
     1861 cgcccgcccg tttggtcagg cgaccgtttg ccataatgcc catgctttcc agccagcgtt
     1921 caaactctgg tgacgggcgc aggttgagta actgacgcac agtggcggta tcgtggaatg
     1981 cggtggtctg gtagttgagc atgatgtcat cacccagcgg catccccatg atgtagttgc
     2041 agcctgcggt ggcgagcagg atcatcaggt tttcgttgag gttctggtca gagtcagcgt
     2101 ggttggtgta gcagcagtca cagcccatag agatgccgct cagcttgccc ataaagtgat
     2161 cttctaagcc agcacggata atctggcggt cgttgtagag atactccggc ccaataaagc
     2221 cgaccacggt gttgacgata aacggatcgt aatgacgcgc cagcccatag ttacgtgctt
     2281 ccatcgtcac ctggtcagcg ccgaagttag cgccagcgga tagcgcagag ccttgtccgg
     2341 tttcgaagta gaggcagttt tccccggcga tacgattgaa ctctgcgccc actgcgcgcg
     2401 cttcgtcgag catcgccagt tccacgccaa actctttcag ccctttttcg ctgccacaga
     2461 tactctggaa aatcagtccg ccaggcgcgc cgcgacgaat cgcttcgatc tgggtggtga
     2521 cgtgcgccag tacgcagccc tgagttggga tgttgaattt gtcgatcacg ccataaatgg
     2581 tatccagcac gctgcttaag ttttccacgt cgtcagttac cgggttaacg ccgatcaccg
     2641 catcgcccac cccgaaggaa agcccttcat agatttgcgc agcgatactt tgcacgtcgt
     2701 cacgggtatc gttcggctgc aaacgggcgc taaaggtgcc cggaataccg atggtggtat
     2761 tggccttttt gattaccggc attttcttcg cgccgtagat caggtccgcg ttggagcaaa
     2821 tcttcgctac cgccgcgacc acttccgagg tcagcccttt gcgggtaaag gcaatgtcgt
     2881 ccacgctggt ttcatcgctc agcacatact cacgcagttc gctgatgctc cagtttttaa
     2941 tctggttgta ggccgtttcg ttaacgtcgt cctgaatcag ccgcgtcacg cagtcatctt
     3001 cataggcaat caccggatta ttgcggatgt ccgctacggt catttccgac aacacctgct
     3061 ttgccgccac gcgctcctgt gagcttgccg ctgcaacgcc cgccagcaca tcccccgaac
     3121 gcagttcgtt ggctttagcc agcacctctt ttacatcctt aaactgatat acattgccga
     3181 acaatgtggt ctttagtttc ataagtcgtt ccctcaggaa gaagtgatga gtaacatttc
     3241 ttccagcatt cgaacaattc tatttcatcg ttagcccttg tcctgtaagg tgttaccctt
     3301 aatacgcccg ttacttattc tgccggacac aaccgaacgc gccggataca tgagcggggt
     3361 tatcgagtcg ttagatcgag gtatccggtt tgagcaagac accactgaca gcaaaggcca
     3421 tagaggccgc ccagccgcag gacaagccct acaaactcac tgattcaatg acgcctggcc
     3481 ttttcttgct ggtacatccc aacggcagca aatactggcg attccggtat tggatagaca
     3541 agaaggagcg gttacaggct attggggtgt atccgctgat tagcctcaag gaagcccgca
     3601 aacgcgccac agagagccgt ttactgatag cccagggaat tgacccaatg gatttggcac
     3661 gcaaggagaa agccattgat gctcttaatg ctatcgccag ttttaaagct gtagcagaag
     3721 actggatggc taccaaggtg agcggatgga gtgagagcta tacaaagcag gtcagatcgg
     3781 cactagagaa agatgtttac cccgtgctag gtaagcggtc gatagtggat ataacggccc
     3841 gtgacgtgct ggcgttacta cagaaaaagg agcgcaccgc accggaacaa gccaggaaat
     3901 tacgccagcg tatcggggag atcttcaaat ttgccgttat cactgaactg gttaaccgga
     3961 atccggttgc agatctggat acggcactga aagccagacg cccaggccat aacgcgtgga
     4021 taccgattaa tgaaattccg gccttctaca aagcccttga gagagctggg agcgttcaga
     4081 ttcagacggc aatacttttg ctttgaggac ggcagaattg cgcctgatgc gctgggaatg
     4141 ggtagatctg caatcggcaa caatcacatt gcccgctgaa gtcatgaagg cccgccgacc
     4201 gcatgttgtc ccgctatccc ggcaggcggt cgagctatta caggaccagt ttacccgcag
     4261 cggatacagt gctttcgtct ttcctggccg attcatggac aagccacttt cagccagtgc
     4321 catccttaaa gccctggagc gtatcggata caagtcgata gccacaggcc acggctggcg
     4381 gaccaccttt tcaacggcgc ttaatgagtc cggcaggtac tcaccagact ggatcgaaat
     4441 ccaactggcc cacgttccga aaggcgtgcg cggtgtttat aaccaggcgg cctacctgaa
     4501 gcaacgacgg gccatgatgc aggattatag cgactgtatt gattcaatac tggctggtga
     4561 cggcaatccg cttgagccgg aatgaataat taagcgggtt tccgacctac tccaccggag
     4621 cagactagat aaatactgga ccccttcaga ataaatattc acctgccgga ttcgtccggc
     4681 ttttgtttat aaatcatcca atcgtgaaat tattagaatt aaacttacat actagtttac
     4741 tagccagatt aggctactag tatataacat caataaaatc atgaggttag tcaaaagttg
     4801 ccgtcagggg tcatgcgggc gtaacattag atcaacgaaa taaaaaagcc cggaaaggcg
     4861 gcaaccagtt ccgggcggta tgagaaacaa gctaaaggag aatttctcat gtctgatatg
     4921 ctagcagtag aacaagaaac aaacaatgat gttcgccaat atttaaacaa aattaacgag
     4981 ttacggaata aagcacccaa aaacgaagag actaaacacg aagaacacac gccagatcat
     5041 tacgaggcaa aacagcagga gcaagcctgg cgtggcaacc tgcgctacct ggacacatta
     5101 aaccgacttg atgaggtatt gcctcgcaaa ctgtacgaaa ggtgggagaa agaacataca
     5161 gtcaacgacg aggccgtgct acgcgccctt tgctactttg ctggtactgg caaaaactca
     5221 cagttgggat ggtgcagggt tggcagggga acaatcgaca agcgagccag gctatcaaaa
     5281 aacacggtta aaaaatgcct cgatcgtctg gtccaccact tcaaactggt tgagcgtact
     5341 gaaggctata tacccggatc ggcagaaagg gaatgcaacg agtaccagct attattcaag
     5401 ccgtacaaca tgaaataacg gaatcaggcg ggcaatgttc ccgccttttc tttgccttaa
     5461 ttccatgaat tcatgggtgg gtcaaaagtt gccgttaggg taggtcaatt tgtgccttta
     5521 ggtgggtcaa aagttgccga gtaagaattt cactattaag aattctaggc gacgggagtt
     5581 ttttagcctc ccggtcggtc acagagagtc gcctaggatt aaaacagaag aatttattgc
     5641 cgcgcagcgc tggatgaatg caatcatcaa taccgctttg gcgcgtcatc cttcccagca
     5701 agctggaaag gcccattcac tcacgcacaa cctgcaaacc agaccaacag accatcatgg
     5761 cgattcgttc atgggtagcg cgggtatatc tcaaagggtg atttcttctt ggtggacctt
     5821 cggaagactt cgagcaattg ccagcttaaa gacagcttag cagtgatggt tatcatcaac
     5881 agctttctga gctaccgcac agacacacag cagcagtaag caaaccgaag ggaatgcaaa
     5941 gcaaaaggat tcgccacact gcaagaccaa tggtttttgt cacgtataca gaccaggcaa
     6001 gcaacagcag caaagcagcg tatagccagg tgcagatagt accatcacac aacgcacagc
     6061 aggctgaggt gaacactcaa gcaactttat ccccgccacc gaccaccgca aactatcctg
     6121 accgtgccaa attcgaacgt aatgccacct ggaacgcgat ggagacacat aagggctacg
     6181 cttgcttagc tgaaaacttc acagcgtcac agcgctattt agacgcatta gagtcgatgc
     6241 gatttcatta aattttttag ccatccagaa atcggcctga ttctgagccg attgccagcc
     6301 ggactactga ccagatcgga ccaatcatcc aaggttggat gaatgaaatc acacgcagat
     6361 cctgccaggc tatttcatcc caccacacag aacgctgaga tctgatatta ccgccgcacc
     6421 attccacgcc agacgaatca aaacaggctt acccttccaa gtggatagct aaaaattaag
     6481 gctgtcagaa tgcgttatat tgcgtttaaa cggcatcgaa ttttaatgtg cgcaatggtg
     6541 ccagattgca ggcaaaaaaa atcccgccgg agaaagcggg gaagtgaaat gaatcgttgt
     6601 agttatagat cacattttga gtaagggtaa ccagcccctg agtaatatta tccctgcatt
     6661 acgtgagatg cgcaattatt ttttgctaat taaaagtttt gtaaacatta cagaatatcc
     6721 cctttcacta tatggtaaaa gatgtgaaac agggacagga ggaatataat attaagtatc
     6781 aacagtaatg ttggccttta atttcattca gggcgcggtg agggttttct ccatattttc
     6841 ctcaccgcta ctttttgagg tgagaacatg aatgatagcg aattaatgac ggttaacgag
     6901 gtagcagctt tattcggcgt tacccgtcgg acaatattcc ggtggatgaa caagattaaa
     6961 ggctggcccg tcccggtcag cccgattgac tcaagaatta agtttatccg atctgagatt
     7021 ttggaattct ataaaaacaa aggcgctcgc caccaatgaa aacagctact gagattatca
     7081 aataacctat tgatttaaaa agctagctac cgcaatttct gaaggaatag gcagcatgaa
     7141 aatcaaagca aacacaatta aagaaataac atcaatcaaa gtaaaaggag cagatgccat
     7201 gcattcatta gacagttatt ttcagcgcac gacagcccct aaatcagccg cccaggaacg
     7261 ccgtgaggaa ttccaggaaa aagtgatgcg ttctgctgat tatattgcgg acaagttcgt
     7321 ggaaacggtt cggccacttg ttgatgaagt agctgataaa cttcagtctg aaatgccgga
     7381 agacatggaa ggcactgcaa aggcgcgact cctttttgaa ttaagtcgtc gtttcggcgt
     7441 gtcaataagc acttttaaat aatcggagga ggaaagacaa tggacgcatt tgatgtactg
     7501 acaaacaaag gcgatgaact ggtagcacag cgtgataaaa ccgccaacga aggggaacgc
     7561 gcactgttaa ataagcagat taaagcgatc cgtatggccc agtttaaatt aatcagcaat
     7621 gaagttatcg aaataattaa accgcagatc ccacaaatcg ttgctgacgc tattaaagcc
     7681 gccggactgg agaaacgcat tgccgggctg aaaacgaacg tagagaaaat ggagatgcac
     7741 aaacagagcg gtgctccgac tccggatgag tacttctcac cggatgctga gtttatggcg
     7801 caggttgagg aacgtctggg tagctgtctc accgaggaac agcgtcgcta ttttgatgga
     7861 gtggatagtt cggcaggtat tgacctgaac tcgtatttcg gtcgtgaaat tgagcatttt
     7921 gatgccgccc cgtctatgcc ggagccagaa ccagaaatgg ccgccgatcc tgaagctgag
     7981 caacggaaac gcgtattcaa tgcgataggg attcggtact gatcggataa aagaaagccg
     8041 ggaattaccc cggctgactt aacaatgtga ccaatgaaca aatcggacaa gacaaacagg
     8101 ataagcagtc tcgaaacaat gttcgggaac tatattagca caagtccgga gaaaatcatc
     8161 atgttggcac tacgcatcat gcagggcatc gctaagaccc tcgcagaaca tgtcttagac
     8221 ctgaaacatt cgccactgag caaacaggcc atgaagcgcc aaacgcttcg tctgtgggct
     8281 gaatattcgc ttggaactat caataaaatc atcgacatga aatcggggcc gagcaaccag
     8341 agcgcggagg aaatggagtt catcaggcgt ttaattctca ttcgcagaga cattcatagc
     8401 cagttacaca gcgtgggaat cgatattaat gatgggacgg gcgattaatt ttcggacaag
     8461 tactatgcgc gcgcgaggag ctgaacatga agcaagatat tgcagacagg ctggagatac
     8521 tcgaaggcca gcgggcagaa gcgaaacaac tacgtaagca ggccagaaga gcgcacagga
     8581 acaacgaagc agagttacta accaaataca tcagtttcac caattactgc atctacgagt
     8641 gttacaagga agatgcagag gactggctgg attcattgcc agaacagtac tgagcagaat
     8701 aacaccacgt catgttgttc tgaggtgagc atatcacctc tgttcaggtg gccaaattta
     8761 gtgtgccact tcagcatcac cgttgaaatc acaataacga tttttttgta aatatgctct
     8821 cacatatgta ggaatagacc aggttggcat tgaaacacat ctaccatgac cataataaag
     8881 taattttttg atatgtttat ggttttatat taattaaaat atatctaaaa ttgatagttt
     8941 taaaattgca aaatccagat tgtgtttgta atgtttaatc tgattgtatt gagtggcgtt
     9001 tattatgaaa aataaatcag gtaattcttg ttatagagaa gcagtcgata ttcatagctc
     9061 acttgagctt tcatttatat tgcataaata tcatttgcgt gtagatgaga agaaatctcg
     9121 aaatgtattc agcgccatac gtgatgagct catccttgat ggggattcac gacaaaactt
     9181 agctactttc tgtcaaacat ggcttgatga tgaaatccgt gagttgctta atttagctat
     9241 tgataaaaac atgatttata aagatgaata tccacaaatg gcggaacctg aaggtcgctg
     9301 tgtgcgtatg ctttccgatt cgtggagttt ccccgattcg cgacacacct taggttgctc
     9361 aaccattggt tcatctgaag ctgctatgct cggccgcttg gcgttaaaat ggcagtggtg
     9421 taaaaaacgc gaagtacaag gtaaatcaac cgaacctgat ttgcgatcct gtgcaaatat
     9481 gctggcataa attctgccgt tactgtgatg tgaaattgtg tgaaattcca cttgatgatc
     9541 atcacctgat aatgaatgct aaagaggttt tgaagcatgt cgatgaaaaa actattggtg
     9601 tcgttacgac tttaggtatt acttattttt gtaaagcaga ttgcgttagt gtttttaaga
     9661 taagtgttaa agttaatatt agtttttata tttacatcgt aattatatgt ttctatttta
     9721 aaaatattcg agagtataaa aatgtctaat gctaatatcg cacccaaaag taagaaaatg
     9781 acagtgggga cactcgctat catgaatata actgctgtag taagtctacg atttatacct
     9841 tctgaagcag aatacggctt aggtgccatt ttttactata cctttgctgc aattatgttt
     9901 ttagtacctg ttgcctttgt tgcggctgag ctagctacaa catatccaca aaaagggggg
     9961 gcatttcgat ggataggaga agcatttggg gctcgctggg gttttgtagg aatgcttatg
    10021 acctggctac aagtcatccc ctactttcca acagtactga catttggtgc agtctcggtt
    10081 gcatttattg atgttgatat gggggtggca gaatcaattg ccagtaataa atggtatatt
    10141 ttatttttcg tactatttgt ttactgggga gctatttgtg ttgcattacg tggcgttggt
    10201 attttctcgc aggtatctaa gtggtgcggt attataggga caattattcc agctattatc
    10261 cttgttattc ttggtttctc ctatctgtgg ttcagcggta aaccagcggt gatcccatta
    10321 ggttggggtg atcttttccc taatatagct aacttccaga atattgttct tgccgccagc
    10381 atatttcttg cttatgcagg gatggaaatg aatgcggtcc atgttaacga tttagataat
    10441 ccaactaaaa aatacccaat tgcaattacc atcgcttcta taggtactat tgcaatattc
    10501 ctgctcagta ctttaggggt cgcctttatt attcctacag ataaaattag tctaactcaa
    10561 agtttgttgg ttgcctacga tatgctgttc gaatgggctg gagtgccatg gcttggctct
    10621 gtgatggcat ttatgctagc aattggcgta ctgggtgggg tggttacttg gattgcaggg
    10681 cctaatacag gtgtattagc gatcgcgaaa gctggttatt tacctaagtt tttccaaaaa
    10741 accaatcgtc atggaatggg gcaccatcta atgtttgtac aaggtataat tgtctcagtt
    10801 ctttctgtta cttttgtgat aatgccttca gttcaagcag cttttcagat tttatcacag
    10861 ttaacagtta tgctctattt agttatgtat atgctcatgt tcgctagtgc tatctatcta
    10921 cgacattctc aacctgatac aaaacgccca tatcgagccc ctgctttatt tttctgggca
    10981 acacttggat tcttagggtc ttttctggct tttgtattaa gttttattcc gcccgagcaa
    11041 atcccaactg gtagtccaac cagctatgtt ctttatttgt tagtattagt tgccctattt
    11101 ttattaattc caatattaat ttacgccttc cgaaaatctg actggaaaga tccaaactct
    11161 gatttcgaac cattttcttg ggaaaaaaga actagtaatt aaattttcca taagctacaa
    11221 aagatgaggt taaatatgtt tagtgctgaa tctccaattg gtgtaataga ctcaggtgta
    11281 ggtggattga catcggtaaa ggaaataatt aatttgcttc ctggtgaaga tattatctat
    11341 tgtggagata atatgaatgc accttatggc aaccgttctg ccgatgatat catcagtttg
    11401 actaaaaaaa tgctgacttt tttacagtca aggaatgtca aactagttgc tgtcgcatgt
    11461 aatactatat cctcaacttt ggaatccgaa gaatacagcg gatatgctaa gagctttccg
    11521 tttcctatct tatccatcat tgaacctgca gtagaagatg tgattcgcca gcaatataaa
    11581 aatgttggta ttatcgctac cgagtttact attaaaacgg ggtgtcacaa agagctaata
    11641 aaaaaattga actcaacaat aaatgtattt ggagaaccaa gcaaaaatct tgcaatgtta
    11701 attgaggaag gtaatcttaa tgctcctgca atcttgaatg acatcaaaaa acatgttaac
    11761 catttaattt cccttcatcc agtaaatgaa attattctcg gctgtactca ctatccaatt
    11821 gttcagaatt tttttgaagc agttgcaccg gatataaaat ttattaatcc tgcacatgat
    11881 caggctatct ctattaaaaa tcacttggga cagctaaatt tattaaactc ctcaaatata
    11941 ggaactttgc aaataaatac tagtggaagt atggagatct acaaaacggt actaggggaa
    12001 ttgagcatta cgaaacctca tacattcagt atcaggcagt tttaaattta tccggtgcac
    12061 cgtaaaaccc cgtccttctg aactgcaccc caaaagttgg acaccaactg agtaaggtgc
    12121 attttatgga caaacccagt tacatactgg gtagtgcatc caattagtag aacatgtgtt
    12181 tttcgataaa cgctccgatc actttttcgt ggatctcaac tgagcgtgag aagcagattg
    12241 ttttacgacc caaccgctta atgcgggtgc gtagcgtcag attattacgc tcaatgcgtt
    12301 gggtgaatat tttgccggtc agatgcttat tcttcggcac ctcccggcca tagctgcccc
    12361 agtcatcgct ggtcagcatg ccgatgttga agggtgtaag cagtgccagt agctcccggc
    12421 acgtttgatc ggttcgggga ccaaaagtgt aggccagtac accgcctgtt ttggtgttgt
    12481 acgcgtaccc gagccagtgt tgccgggctt tactgccaac gtagctccat tgctcatcaa
    12541 gctcgcagat aaacgccaca tcagcatggg caacaggcga agaagttatt cgctttggcg
    12601 cgagtttttt aaagtccgga tgacggtgtt actaccaatt ttcagtgtcc tggcggtatc
    12661 gcgaaccccg gcaccattaa aggccatttc agttatcagc tctttcatac ccggcttacg
    12721 tgcttgataa gtataagtga actgaaacac acggtggcag tcacggcagc gaagtctgtc
    12781 acggccttta gggttctgac catggcggta aacctgagct gactgacaac ggggacaatg
    12841 aatattaacg ctggtcatga gataacctca aaagcccgta ttatacatca gattcaacta
    12901 attagatgca tcacccatcc agcattataa aacatactaa caataactaa attataataa
    12961 tcacaactta caatcaaata ataaatgcat aagattaaca atgatttttt ctggttatat
    13021 ttatctaaaa aagcagaata ttatgtaact tattattttc taagcgaaaa tttattgtgc
    13081 ggtcagacta acaaacttgc gtaccacccg aagaacatgc gaagatttaa gtattctttc
    13141 ttttttggtt tttatttttc attttctctt ttcttctctt tccggttctc ttcttttctg
    13201 gttttggttt tttccttttt atttttattc tttcttcagg gttatgctaa aacaccaaaa
    13261 catttttcac cacaaaagca tccaatcatc ttgccagacc aatcacctta accggataca
    13321 ctgccggata taatcccact aacacccagc caccaagcac catacctgat aactaccgta
    13381 ttcaggaagg aaatgcgagt gatttcaccg tcaccggcac aaccgatccg ccaaaaagag
    13441 gcgtaccaat gtcgatatag tcccccgcgc ggacaatcac ttcgtcaatg actgccaacg
    13501 ggagttgttg tagctgtggg cgcaacaaca tgcccagagc tttaccaaag tcctgcccgg
    13561 ccaccaccag caggggatgc ggattcggaa aacgcgcgac gaaatcgacc agcgcgttga
    13621 tgaccgtcag taccgcggcg taacgcacag gcagcgaggc gggaagcgcc agcacgtacg
    13681 cgtcagtttt gggatcaaga tccagctgaa gcagcgcctg ttgccaggca ctcaccagat
    13741 ccgtttcatc aatcgggatc gccaccggca aattgcgcag cggcagttgt acgccctcca
    13801 gccagattgt gctgccagag agcgaaaggg tatgtgcacc cgcgccaatc accgtggcgc
    13861 gtacggtttg cgccggaaac tgcacattca tctcacgcag gcgcggatgg tcatgcagcg
    13921 ccgttgccag aagtgggcca atatcggcaa aacagaacgg gtcggcgggc tggtggcgat
    13981 aacattcgcc cacgcctcca gaaagcgtaa tgatttcggg cgtaacacct gcgggcagca
    14041 aaccggtttg catcaatgcc tgcgcgagcg gcgaaagcgt tccgtcaatc acttcgacaa
    14101 tcagcgccgc catccgccgg gtaacctgca caagctgcgc gccggtcagc gaacgggcgt
    14161 cagtgcctgc accgaagcac tcatccacaa tcatctgccc cggtttatga gcgtaaacca
    14221 cgcgcccctg gctgtcggtt tccagcaggc gaccaccgac gttgagacag gcagtgccgc
    14281 tgatttttcc ggcatcgaac agggcgtagt tcgcggtgcc accgccgata tcgatattca
    14341 gtacccgaca cagccgttgt tcagaaaggg tttgcgcccc ggctccgtga ccggcgatca
    14401 cggattcgag gtgcggcccg gcgctggcga cgacaaaatc gcccagcgac tgagagagcg
    14461 ccatcaccgc cgggcgagca ttgcgggttt tcgcgctttc accggtgatg atgatggcac
    14521 cagaatcaac gctttccggc gcaacgcccg cagcctgata ttgctctaaa atcagcgatt
    14581 tcagttccgc ttcttttaaa ccaccctgtt tatcgacagg ggtaaagaac accgggcttt
    14641 gccagctaat ttcgcgttta atgaattcgt agcgcggcac ctgcgacacc gccgcacggt
    14701 taaccagctc caggtgggag aaaatcacct gggtggtggt ggtgccgata tcgataccga
    14761 cgctcaatag ctggcgagtg ttcacgattg tgcctccgct tcggttttag tcgcggtcgc
    14821 gtcttctttt ggcaccagca tcatcgccac gccaatcgcc gttacgccgc cgatcaactt
    14881 gccgacaatc atcgggaaga tcatggcgtt catgttggca gcggcgaagc ctaagtggtc
    14941 gcccagggcg aaagcagcgg aaacggcgaa ggcgcagttg atgactttgc cgcgggtatc
    15001 catctgcctc atcatgccga acatcgggat gttgttggca agcgttgcca ccatgccggc
    15061 tgccgcgatg ttgttcatat tcagcacttt accgacgctc atcagcggtt tttcaaacca
    15121 gcgggtcagc agcagcacca ttggatacgc ccctaacaga acgcaggaga tagaaccgat
    15181 aacttcaatg gcgcgcatca cttcaccggg tttatcgcca ggggccataa agatagggtc
    15241 cagaccgggg atcagttccc agccaagcag aaacttaact accgcggcag caagaccgag
    15301 ggtgatcaat gcaacgagga atttggcgaa gatctggaag ccgttgatca ttttttccgg
    15361 gatgaatttc agccccagcg ccaccagtac cgcgacgata agcaccggga tcatgttcat
    15421 caggatgagc gcaaaggtga attccaccgg ctggccgttg atctgtacac cggagtacat
    15481 agccaccagg ccaccggcga tacagccaat tggaatggtc acaatgcccg ccagcacgcc
    15541 gagcgccaga taacgacgat cagaaggttc gataatgccg agcgccaccg gaatggaaaa
    15601 cacaatcgtt ggccccatca tcgacccgag aattaaccca gagtatagcc acgctgctac
    15661 gtcgccgccc gccagctctt tggcgaggaa gaagccgccc atatcgcacg ccagcagtgt
    15721 tccggcgaac attgacgggt ttgcaccgag catttcgtaa accgggataa ttaccggccc
    15781 gagtacgtga gccagtaccg gtgccagcgc ggtcataccg accatcgcca gacccagagc
    15841 gcccattgcc ataaagcctt cttcgaactg accgcctgat ccttcgatac ttttaccgaa
    15901 cttaccgagg aaacgagcag aaccgccgaa ctgcgacagg atcctgtcta cggcagctat
    15961 cagcataaag aacatcatga tgtacatgat gatttcgtta attcccatcg cctttactcc
    16021 ctgttagttg ttatttattg gcggatgcgg cgtaaacgcc ttatccgccc tacatgtgca
    16081 atcccgaagg ccggatcaga cgcggcaagc
//
LOCUS       DADLSA010000089        16067 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.089, whole genome shotgun sequence.
ACCESSION   DADLSA010000089 DADLSA010000000
VERSION     DADLSA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.089"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..1106
                     /locus_tag="JN691_002743"
     CDS             <1..1106
                     /locus_tag="JN691_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HAY7244151.1"
                     /translation="TEKVVGLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGA
                     IKGVGEGPIEAIIEARNKGGYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHR
                     AALMNSLGDALKAADQHAKAEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGE
                     RETLGLYLTGHPINQYLKEIERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRG
                     NRIGICTLDDRSGRLEVMLFTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTARE
                     VMDIDEAREKYARGLAISLTDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARAR
                     LRFGATWRVSPSDRLLNDLRGLIGSEQVELEFD"
     gene            1119..2078
                     /gene="accA"
                     /locus_tag="JN691_002744"
     CDS             1119..2078
                     /gene="accA"
                     /locus_tag="JN691_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAY7244152.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            2177..4318
                     /gene="ldcC"
                     /locus_tag="JN691_002745"
     CDS             2177..4318
                     /gene="ldcC"
                     /locus_tag="JN691_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAY7244153.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            4375..4764
                     /locus_tag="JN691_002746"
     CDS             4375..4764
                     /locus_tag="JN691_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7244154.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            4829..6127
                     /gene="tilS"
                     /locus_tag="JN691_002747"
     CDS             4829..6127
                     /gene="tilS"
                     /locus_tag="JN691_002747"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAY7244155.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(6176..6436)
                     /gene="rof"
                     /locus_tag="JN691_002748"
     CDS             complement(6176..6436)
                     /gene="rof"
                     /locus_tag="JN691_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAY7244156.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(6423..6623)
                     /locus_tag="JN691_002749"
     CDS             complement(6423..6623)
                     /locus_tag="JN691_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAY7244157.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            6789..7334
                     /locus_tag="JN691_002750"
     CDS             6789..7334
                     /locus_tag="JN691_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HAY7244158.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            7331..7753
                     /gene="arfB"
                     /locus_tag="JN691_002751"
     CDS             7331..7753
                     /gene="arfB"
                     /locus_tag="JN691_002751"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY7244159.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            7767..8477
                     /gene="nlpE"
                     /locus_tag="JN691_002752"
                     /gene_synonym="cutF"
     CDS             7767..8477
                     /gene="nlpE"
                     /locus_tag="JN691_002752"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAY7244160.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(8632..9456)
                     /locus_tag="JN691_002753"
     CDS             complement(8632..9456)
                     /locus_tag="JN691_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HAY7244161.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(9509..11227)
                     /gene="proS"
                     /locus_tag="JN691_002754"
     CDS             complement(9509..11227)
                     /gene="proS"
                     /locus_tag="JN691_002754"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAY7244162.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYNKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(11338..12045)
                     /gene="tsaA"
                     /locus_tag="JN691_002755"
     CDS             complement(11338..12045)
                     /gene="tsaA"
                     /locus_tag="JN691_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAY7244163.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(12042..12446)
                     /gene="rcsF"
                     /locus_tag="JN691_002756"
     CDS             complement(12042..12446)
                     /gene="rcsF"
                     /locus_tag="JN691_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAY7244164.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(12564..13379)
                     /gene="metQ"
                     /locus_tag="JN691_002757"
     CDS             complement(12564..13379)
                     /gene="metQ"
                     /locus_tag="JN691_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAY7244165.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(13419..14072)
                     /gene="metI"
                     /locus_tag="JN691_002758"
     CDS             complement(13419..14072)
                     /gene="metI"
                     /locus_tag="JN691_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAY7244166.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(14065..15096)
                     /gene="metN"
                     /locus_tag="JN691_002759"
     CDS             complement(14065..15096)
                     /gene="metN"
                     /locus_tag="JN691_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAY7244167.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDDIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            15284..15859
                     /gene="gmhB"
                     /locus_tag="JN691_002760"
     CDS             15284..15859
                     /gene="gmhB"
                     /locus_tag="JN691_002760"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAY7244168.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3907 a         4040 c         4310 g         3810 t
ORIGIN      
        1 acaccgagaa ggtggtgggc ctggtggatg agtgctggcg gatggggctg aaaatcctgc
       61 caccagatat aaactccggt ctttaccatt tccacgtcaa cgacgacggc gaaatcgtgt
      121 atggtattgg cgccatcaaa ggggtaggtg aaggtccgat tgaggccatc atcgaagccc
      181 gtaataaagg cggctacttc cgcgaactgt ttgatctctg cgcccgtacc gacaccaaaa
      241 agttaaaccg gcgggtgctg gaaaaactga tcatgtccgg ggcgtttgac cgtcttgggc
      301 cacaccgcgc ggcgctgatg aactcgctgg gtgatgcgtt aaaagcggca gatcaacacg
      361 cgaaagcgga agctatcggt caggccgata tgttcggcgt gctggccgaa gagccggaac
      421 aaattgaaca atcctacgcc agctgccaac cgtggccgga gcaggtggta ttagatgggg
      481 aacgtgaaac gttaggcctg tacctgaccg gacaccctat caaccagtat ttaaaagaga
      541 ttgagcgtta tgtcggaggc gtaaggctga aagacatgca cccgacagaa cgtggtaaag
      601 tcatcacggc tgcggggctc gttgttgccg cgcgggttat ggtcaccaag cgcggcaatc
      661 gtatcggtat ctgcacgctg gatgaccgtt ccgggcggct ggaagtgatg ttgtttactg
      721 acgccctgga taaataccag caattgctgg aaaaagaccg catacttatc gtcagcggac
      781 aggtcagctt tgatgacttc agcggtgggc ttaaaatgac cgctcgcgaa gtgatggata
      841 ttgacgaagc ccgggaaaaa tatgctcgcg ggcttgctat ctcgctgacg gacaggcaaa
      901 ttgatgacca gcttttaaac cgactccgtc agtctctgga accccaccgc tctgggacaa
      961 ttccagtaca tctctactat cagagggcgg atgcacgcgc gcggttgcgt tttggcgcga
     1021 cgtggcgtgt ctctccgagc gatcgtttat taaacgatct ccgtggcctc attggttcgg
     1081 agcaggtgga actggagttt gactaataca ggaatactat gagtctgaat ttccttgatt
     1141 ttgaacagcc gattgcagag ctggaagcga aaatcgattc tctgactgcg gttagccgtc
     1201 aggatgagaa actggatatt aacatcgatg aagaagtgca tcgtctgcgt gaaaaaagcg
     1261 tagaactgac gcgtaaaatc ttcgccgatc tcggtgcatg gcagattgcg caactggcac
     1321 gccatccgca gcgtccttat accctggatt acgttcgcct ggcatttgat gaatttgacg
     1381 aactggctgg cgaccgtgcg tatgcagacg ataaagctat cgtcggtggt atcgcccgtc
     1441 tcgatggtcg tccggtgatg atcattggtc atcaaaaagg tcgtgaaacc aaagaaaaaa
     1501 ttcgccgtaa ctttggtatg ccagcgccag aaggttaccg caaagcactg cgtctgatgc
     1561 aaatggctga acgctttaag atgcctatca tcacctttat cgacaccccg ggggcttatc
     1621 ctggcgtggg cgcagaagag cgtggtcagt ctgaagccat tgcacgcaac ctgcgtgaaa
     1681 tgtctcgcct tggcgtaccg gtagtttgta cggttatcgg tgaaggtggt tctggcggtg
     1741 cgctggcgat tggcgtgggc gataaagtga atatgctgca atacagcacc tattccgtta
     1801 tctcgccgga aggttgtgcg tccattctgt ggaagagcgc cgacaaagcg ccgctggcgg
     1861 ctgaagcgat gggtatcatc gctccgcgtc tgaaagagct gaaactgatc gactccatca
     1921 tcccggaacc gctgggcggc gctcaccgta acccggaagc gatggcggca tcgttgaaag
     1981 cgcaactgct ggcggatctg gccgatctcg atgtgttaag cactgaagat ttaaaaaatc
     2041 gtcgttatca gcgcctgatg agctacggtt acgcgtaatt cgcaaaagtt ctgaaaaagg
     2101 gtcacttcgg tggccctttt ttatcgccac ggtttgagca ggctatgatt aaggaaggat
     2161 tttccaggag gaacacatga acatcattgc cattatggga ccgcatggcg tcttttataa
     2221 agatgagccc atcaaagaac tggagtcggc gctggtggcg caaggctttc agattatctg
     2281 gccacaaaac agcgttgatt tgctgaaatt tatcgaacat aaccctcgaa tttgcggcgt
     2341 gatttttgac tgggatgagt acagtctcga tttatgcagc gatatcaatc aacttaatga
     2401 atatctcccg ctttatgcct tcatcaacac ccactcgacg atggatgtca gcgtgcagga
     2461 tatgcggatg gcgctctggt tttttgaata tgcgctgggg caggcggaag atatcgccat
     2521 tcgtatgcgt cagtacacca acgaatatct tgataacatt acgccgccgt tcacgaaagc
     2581 cttgtttacc tacgtcaaag agcggaagta caccttttgt acgccggggc atatgggcgg
     2641 caccgcatat caaaaaagcc cggttggctg tctgttttat gattttttcg gcgggaatac
     2701 ccttaaggct gatgtctcta tttcggtcac cgagcttggt tcgttgcttg accacaccgg
     2761 gccacacctg gaagcggaag agtacatcgc gcggactttt ggcgcggaac agagttatat
     2821 cgttaccaac ggaacatcga cgtcgaacaa aattgtgggt atgtacgccg cgccatccgg
     2881 cagtacgctg ttgatcgacc gcaactgcca taaatcactg gcgcatctgt tgatgatgaa
     2941 cgatgtagtg ccagtctggc tgaaaccgac gcgtaatgcg ttggggattc tgggggggat
     3001 cccgcgccgt gaatttactc gcgacagcat cgaagagaaa gtcgctgcca ctacgcaagc
     3061 acaatggccg gttcatgcgg tgatcaccaa ctccacctat gatggtttgc tctacaacac
     3121 cgactggatc aaacagacgc tggatgtccc gtcgatccac ttcgattccg cctgggtgcc
     3181 gtatactcat tttcatccaa tctaccaggg taaaagtggt atgagcggcg agcgtgttgc
     3241 gggaaaagtt ttcttcgaaa cgcaatcgac ccacaaaatg ctggcggcgt tatcgcaggc
     3301 ttcactgatc cacattaaag gcgagtatga cgaagaggcg tttaacgaag cctttatgat
     3361 gcataccacc acctcgccca gttatcccat tgttgcttcg gttgagacgg cggcggcgat
     3421 gctgcgtggt aatccgggca aacggctgat taaccgttcg gtggagcggg cgctgcattt
     3481 tcgcaaagag gtccagcggc tgcgggaaga gtctgacggt tggtttttcg atatctggca
     3541 accgccgcag gtggatgaag ccgaatgctg gcccgttgcg cctggcgaac aatggcacgg
     3601 ctttaacgat gcggatgccg atcatatgtt tctcgatccg gttaaagtca ctattttgac
     3661 accggggatg gacgaacagg gcaatatgag tgaggagggg atcccggcgg cgctggtagc
     3721 aaaattcctc gacgaacgtg ggatcgtagt agagaaaacc ggcccttata acctgctgtt
     3781 tctctttagt attggcatcg ataaaaccaa agcaatggga ttattgcgtg ggttgacgga
     3841 atttaagcgc tcttacgatc tcaacctgcg gatcaaaaat atgctgcccg atctctatgc
     3901 tgaagatccc gatttctacc gcaatatgcg tattcaggat ctggcgcaag ggatccataa
     3961 gctgattcgt aaacacgatc tttccggttt gatgttgcgg gcattcgata ctttgccgga
     4021 aatgatcatg acgccacatc aggcatggca gagacagatt aaaggtgaag tagaaaccat
     4081 tgcgctggaa caactggtcg gtagagtatc ggcaaatatg atcctgcctt atccaccggg
     4141 cgtaccgctg ttgatgccag gagaaatgct gaccaaagag agccgcacgg tactcgattt
     4201 tctactgatg ctttgttccg tagggcaaca ttaccccggt tttgaaacgg atattcacgg
     4261 cgcgaaacag gacgaagacg gcgtttaccg cgtacgagtc ctaaaaatgg cgggataact
     4321 tgccagagcg gcttccgggc gagtaacgtg ctgttaacaa ataaaggaga cgttatgctg
     4381 ggtttaaaac aggttcacca tattgcgatt attgcgacgg attatgcggt gagcaaagct
     4441 ttctactgcg atattcttgg cttcacgctg caaagcgaag tctatcgcga agcgcgcgac
     4501 tcgtggaaag gggatttggc gcttaatggg caatatgtga ttgagctttt ctcttttccg
     4561 ttcccgccgg agcgccccag ccgaccggaa gcttgcggcc tgcgtcatct ggcttttagc
     4621 gttgatgaca tcgatgcggc agtggcgcac cttgaaagcc ataacgtgaa gtgtgaagcc
     4681 atccgtgtcg atccatacac gcaaaaacgc ttcaccttct ttaacgatcc ggacgggctg
     4741 ccgttggaac tgtatgagca gtaaggcttg tcatcgccgc atttgcccgg taacgtgccg
     4801 ggcattgcta ctgtaaaatc gcaccatcat gacactcacg ctcaatagac aacttctcac
     4861 ctcacgccag attctggtgg cctttagcgg cgggcttgac tccaccgttc tgctgcatca
     4921 gttggtgcag tggcggacgg aaaatccggg cgtcactctg cgcgctattc atgtgcatca
     4981 cggtttaagt gctaatgccg atgcctgggt tacgcattgc gaaaacgtct gccaacagtg
     5041 gcaggtgccg ctggtggtcg aacgcgtaca acttgcgcaa gaggggctgg ggattgaggc
     5101 ccaggcgcgg caggcgcgtt atcaggcatt tgcccgtacc ctattgcccg gcgaagtgct
     5161 ggtcaccgcg caacatctcg acgatcaatg tgaaaccttt ctgctggcgc taaaacgcgg
     5221 tagcggccct gccgggcttt cggctatggc ggaagtttcg gagtttgctg gaacacggct
     5281 tatccgcccg ttgcttgccc gcacgcgggg ggaactggag cagtgggcat tggcgcatgg
     5341 tttacgctgg attgaagacg aaagtaatca ggacgactca tacgatcgta actttctgcg
     5401 cctgcgcgta gtgccgttat tgcagcagcg ttggccgcat tttgcggaag caacggcccg
     5461 cagcgccgca ctttgtgctg aacaagagag tctgttggat gaactgctgg cggatgattt
     5521 agcacactgc caaacgtcgc aggggacgct gcagattgcg ccaatgctgg cgatgagtga
     5581 tgcccgtcgc gcggcgatta ttcgccgctg gctggcaggg cagaatgcac cgatgccttc
     5641 ccgcgacgcg ctggtgcgca tctggcagga agtggcgctg gcgcgggaag atgcttcccc
     5701 ctgtttacgt ttgggcgcct ttgaaatccg ccgctatcag tcgcaactgt ggtggattaa
     5761 atccgtcacc gggcaaagcg aaaccattgt gctgtggcag acgtggctac aaccgctgga
     5821 attaccggcg gggctgggaa ccgtacagct tactgcggga ggcgatattc gccctccgcg
     5881 tgcagacgaa gcggtcagcg tgcgtttcaa agcgccagga ttgctgcata ttgtcgggcg
     5941 caacggcgga cgtaagctga agaaaatctg gcaagagtta ggcgtgccgc cgtggctacg
     6001 tgacaccacg ccactgctgt tttatggcga aacgctgatt gcggcggcag gggtatttgt
     6061 gacgcaagaa ggtgtggctg aaggtgagaa tggcgtaagt tttgtctggc agaaaacgct
     6121 tagttaagtg aaagccggat aagacgcatc aaacgtcgca tccggcgaaa gtcaatcagg
     6181 attcgcttac caccaccgta ccgatttcag ggtggctaaa gctggtaatt ttatccagac
     6241 gcagctcgcg ggtttcgcca gcggcctcga cgaccagata ctccacattt ttgcgggaga
     6301 ctaaatcact ggcttttgcc tgcaattttt cgccatcttt cagctcaagt gtcagcatta
     6361 aatgatgctg gcaggcgagc tcaagattat cgtaatcatc acaattaatt ggttgatacg
     6421 tatcattcat tgacataatc gctcaccagt aagtttgccg cagcgtatgc tgctttttcc
     6481 ctgacagcct cagaaagggc gtcgtcggca gccatttcat tcagcacttt caaaacgcag
     6541 cccagcgcgt ccggaacgta tcctaagtct ccgctggcga tttccgcgta ccgcttgcgt
     6601 attaactcac aatatttttc cacatgccct cctgtcagca ctctgactta accgtggatg
     6661 caagtctaag cctacgaaga taaactctgt ttcgcaaggt gactatacca cactcatttc
     6721 tgcaatatca gcgccgcaac tgcacgtatt ccgttacaat ggcctcctga ttcgaaagga
     6781 gttttcttat ggcgcttaaa gcgacaattt ataaagcgac ggttaatgtg gccgatctcg
     6841 accgcaacca gtttctcgat gcctctctga cgctggcgcg ccacccttca gaaacccagg
     6901 agcgtatgat gctgcgcttg ctggcgtggc tgaaatatgc tgatgaacgt ctgcaattta
     6961 cccgtggttt gtgtgccgat gatgagccgg aagcgtggct gcgtaacgat cacctgggca
     7021 ttgatttgtg gattgagctg gggctgccgg atgagcgacg aattaagaaa gcctgcaccc
     7081 aggccgcaga agtggcgctg tttacctata atagtcgggc ggcgcaaatc tggtggcagc
     7141 aaaatcagag caaatgtgtg cagtttgcca atctttccgt ctggtatctg gacgatgaac
     7201 aactggcgaa agtaagcgcc tttgccgatc gtaccatgac gctgcaggca acgattcagg
     7261 atggtgttat ctggttatcg gatgataaga ataatctgga agttaactta accgcctggc
     7321 aacaaccttc atgattgtga tttcccgaca tgttgctatt cccgatggtg agcttgagat
     7381 caccgccatt cgtgcgcagg gcgctggcgg gcagcatgtt aataagacct caacggctat
     7441 tcatctgcgt tttgacattc gggcgtccag cctgccagag tattacaaag agcgtctgct
     7501 cgccgccagc catcatttga tcagcagtga tggcgtgatt gtcattaagg cacaggaata
     7561 ccgcagtcag gaactgaacc gcgaagccgc tctggcccgg ctggtggcag tgattaaaga
     7621 tttaacaaaa gaacaaaaag cccgacgacc cacgcggccc acccgtgcat cgaaagagcg
     7681 caggctggca tcgaaagcac aaaaatcaag cgtgaaggcg atgcgcggca aagtacgcag
     7741 cggtcgggaa taaaaagaag gaatggatgg tgaaaaaagc gatagtgaca gcgatggctg
     7801 taatcagcct ctttactctg atgggatgta ataatcgggc cgaagtcgat acgctttctc
     7861 cggcgcaggc tgccgaactg aaaccgatgc cgcaaagttg gcgcggcgtg ctaccgtgtg
     7921 ccgattgcga aggaatcgaa atctctctgt tcctcgaaaa agacggaaca tgggtgatga
     7981 atgagcgtta tctcggtgct cgtgaagaac cttcctcctt cgcttcctac ggtacatggg
     8041 cgcgaaccgc tgacaagctg gtattaaccg acagcaaagg tgaaaagtca tattatcggg
     8101 cgaaaggcga tgcgctggag atgctcgatc gtgaaggcaa tccaattgaa tcgcagttca
     8161 actatacgct ggaaccggca caatccagtt tacctatgac gccgatgacc ctgcgaggca
     8221 tgtattttta tatggctgat gcggcgacct tcactgattg cgcgaccgga aaacgtttca
     8281 tggtagcgaa taacgcagag ctggagcgta gctacctggc tgcgcgcggt cacagtgaaa
     8341 aaccggtgtt actgtcagta gaaggtcact ttacgcttga ggctaatccg gatactggtg
     8401 cgccaaccaa agtattagcg cccgatacgg cgggtaaatt ttacccgaat aaggattgca
     8461 gtagtttggg gcagtaaacc tgcttaaggc agaaacaaac gcaaaattgc ctgatgcgct
     8521 gcgcttatca ggcctacagc atataatgca acattttgaa ttagcacgat tttgtaggcc
     8581 ggataaggcg tttacgccgc atccggcatt ttgtgcacga ggctggcttt atcactgagt
     8641 cagcccaaca aagcgacccg ccttgatata aatcccaggc ttcaggtgcc caacatactg
     8701 taactgcgtt tctggcttaa acgcagagac atcaccaata cgcatatgca tcagatcggc
     8761 agggctaatc cagcccgatt gcgccagtgt aagtggatgc ccggctttgg caaatgcatc
     8821 agtgacaaat tccgaacaaa accacgactt tttgtctcct tcgcacacac tgcttaactg
     8881 cgctttcgcc aggccgctga cgcactgttg gcgaaaatcc tcggagaacg gattcagtga
     8941 gcacatctgg cgagtcacca taaagggaat aaattcgaca atgccgcgat agttataacc
     9001 gctatctttg attttattgg caaacgcggt gatatctgtg gcttgttgcg gggtaagatc
     9061 cgggactcgt aagacgaaaa gcttatcact atgcttcatc gcttttttaa gggaaacaat
     9121 ctggacgcca gcgcctgtcg cttctgcaac gttattatca cccagaaaga ttgcaacgtg
     9181 actcacagag gaagtgctga agacgcggat tccaaatgag gttaccccaa ggcttgagga
     9241 gaacagcaaa tcgccgggtt tgagatcagg tgccgttatt tcttttattg attgttcggt
     9301 gaaagagctt tgatgctgga atttgacagc ccatgttttt gcctcggcat ctactgctgt
     9361 ggctgatggg tctggctggc tgatatcaac ggtacaagcc gaaagtaaaa gaaagctggg
     9421 gagaagcagg cggcagtacg cctttggttt atccatttta tacaatccat gtaaaaaagg
     9481 gccctgaaat tcaggaccct ttctggcatc agcctttaat ctgtttcacc agatattcga
     9541 cgatgtcacc agtcttaatt aactgtttct cgccgttgcg acgatattta tattcgatat
     9601 cgtcgttgtc gaggttacgg tcgcccagca caatagtgtg cggaataccg atcagttcca
     9661 tatcagcaaa catcacgccc ggacgctctt tgcggtcatc cagcagcact tcgatacctt
     9721 gtgcacgcag ttcgctgtac agtttctcag caagctcttg tacgcggaag gatttgtgca
     9781 tgttcatcgg cagaatcgcc acctggaacg gcgcgatagc gtcaggccat acgatgccgc
     9841 gttcgtcgta gttctgctca atcgccgcag ctaccacacg cgttaccccg ataccgtagc
     9901 aacccatcgt caggatttgg ttacggccat cttcaccctg tacggaggct ttcagtgctt
     9961 cggagtactt ggtacccagc tggaagatgt gaccaacttc gataccacgt ttgatcagca
    10021 gcgtaccctg gccatccggg cttggatcgc cagccaccac gttacggata tctgcaactt
    10081 ccggggtagc gacatcgcga tcccagttga taccgaagta gtgtttacca tcgatgttag
    10141 caccagcagc gaaatcactc atcgccgcaa cggtacggtc aatcaccacc ggaatcggca
    10201 tgtttaccgg acccagtgaa cccggaccgg ctttaaccac agcacgaatt tcttcttcgg
    10261 tcgcgaaagt cagcgggctt gcaacctgcg gcagtttttc tgctttaact tcgttcagct
    10321 cgtgatcacc gcgcaccagc agcgcaacca gcgggaagct actgccttca accgctttaa
    10381 ccagcagagt cttaaccgtt ttctcaatcg gcagattgaa ctgttcaacc agttccgcga
    10441 tggttttcgc gttcggcgta tcaaccagcg tcatttcctg ggtagcagca gcgcgcggtt
    10501 ctttcggcgc gatagcttct gccagttcaa tgttcgctgc atagtcagag gtgtcggaga
    10561 agaccacatc gtcttcaccg ctctgcgcca gcacctggaa ttcgtgagag gcgctgccgc
    10621 cgatagaacc ggtgtcggcc tgtacggcgc ggaaatccag ccccatgcgg ctgaagattt
    10681 tgttgtaggc cgcatacatt gcatcgtagg tttcctgcag ggattcctga gaagtatgga
    10741 aagagtaagc atctttcatc aggaattcgc gggaacgcat gacgccgaaa cgcggacgca
    10801 cttcgtcgcg gaacttggtc tggatctgat agaagttcag cggcagctgt ttgtaagagc
    10861 taagctcgtt acgaatcagg tcagtgataa cttcttcatg agttgggccg agtacgaacg
    10921 gacgctcgcc acggtcaaca aaacgcagca gttccggacc gtactgttcc caacgaccac
    10981 tctcttgcca caaatcggct ggctgaacca ccggcatcga cacctcgatc gcaccggcgt
    11041 tgttcatctc ttcacgcacg atgttttcga cttttttcag aacgcgcacg ccggtcggca
    11101 gccaggtata taacccggag gccagcttgc ggatcatccc ggcgcgcagc atcagctgat
    11161 ggctgatcac ctcggcgtcg gcaggtgtct ccttgagagt ggagagcagg tattggctag
    11221 tacgcatgtt gttacggttc cagttggaag gtagaacagg ctcaaggcga gcctgggaca
    11281 aaaaaagtga tttagtttac cagtgcaaaa gaaatgtcaa aagagaaggg cgtgaattta
    11341 acgcggttcc agcgcaaaga cttcaaaacc tgcgtcggtg acgcgccagc gaacgttaaa
    11401 atcatgtagc cagacggcat aggttttgcc cgtttcctca cctttacgat aggccgggcg
    11461 cgggtcctgc gccagtactt cgcggataaa cagcgttaac tgcggataac gcttctccag
    11521 cgtcaaaagc tgcttttcga cctctgcggt aaaactcacc gccatctctg cagctggcgc
    11581 actttgcgca tagctggcac tggcatcggg aagcgattcg gcaaagggga gatacggttt
    11641 gatatccact accggcgtac catcgaccag atccagactg ccgagcttca gaatcacgct
    11701 gtctttatgg caaacaacct ctttcagctc taccagcgac atgccaattg ggttagggcg
    11761 gaaagtagag cgtgttgcga aaacccccat tctggcgtta ccgccgaggc gcggtggacg
    11821 cacagtcgga cgccagccgc cttccatcgt ttgatgaaag acgaaaagga tccataaatg
    11881 gctgaatgct tccaggccgc gaacggcgtc ggcctggttg taggaagcaa tgagatgcag
    11941 ttcgccgttg gcgcttttta ccagacctgg ctggcgcgga acggcgaatt tttccttata
    12001 gggcgagcga ataacgccta tttgctcgaa ctggaaactg ctcatttcgc cgtaatgtta
    12061 agcgcagagc cgatacatac agcctgacga tagcagcccg gcgtaccgct ggtgacttcg
    12121 cagctatgca gtaatacagc attggctttc attttagagg cattgatttg catctgctta
    12181 cgtgcggttg gaatgctcgg cggagagtcc tgattagagg cctggcaaga gtcgccactg
    12241 acttcaccga gatcgcggaa cggtttgccg actaattctt ctgcattggt ataaattcgg
    12301 accggcgtgg cgcgcggcgc tttcggtttt gcaggctccg ctttcggctg gggtgcagtg
    12361 ctttgaacgg gttcgacagg ggatctgctt aacatggaac agccgcttag catgagtgct
    12421 actaaacaga tcggtaaagc acgcatagta tttcctcaat gtatgatcaa aacgtcaata
    12481 ttgaatcagg agcttgtaaa aatgacaaga cgggcaagcg cccgtcctga atgatattac
    12541 aaattgtgga aacagcctaa aaattaccag cctttaacag cgccgccgtt aaacactttg
    12601 tttgctgctt cgtaaacttc atcagactga taagcctgga cgaatttctt cacgttctcg
    12661 gcgtctttgt tatcttcacg cgttacgatc aggtttacgt atggggagtc tttatcttcg
    12721 acaaagatac cgtctttcgc cggagtcagg ccaatctggc tggcataggt ggtattgata
    12781 actgccagag cgatttgcgc gtcgtccaga gaacgcggca gttgcggtgc ttccagttca
    12841 acaattttca gattttttgg gttctcaaca acatcaagaa cggtcggcag caggccaacg
    12901 ccatctttca gtttgatcaa gcccactttt tgcagcagca gcagtgaacg accaagatta
    12961 gttggatcgt ttggcacggc aacctgcgaa ccatcctgca gttcatccag tgatttgatt
    13021 ttcttggagt aaccagcaat cggataaaca aaagtgttgc ctactgcgac cagtttgtag
    13081 ccacgatctt tcagttgctg atcaaggtac ggtttatgct ggaaggcgtt ggcgtcgata
    13141 tcgcctttgc tcaatgcttc gtttggcaga acatagtcgt tgaaggttac cagctcaacg
    13201 tccaggccat atttgtcttt cgcaactttc tgcgcgactt ctgctacctg ctgttcggca
    13261 ccaacaatca cgccgacttt aatgtggttt ggatcttttt catcctgacc gcagcctacc
    13321 agtgccagtg atccaatcag ggctcccact gccgcaaagg ttttgaattt gaacgccata
    13381 ccttattcct tcttcaatta tttatgttgt gttgaacgtt acttgcgagt gacagcccgg
    13441 acgatgcggt cgcctgcgaa ctgaattaaa taaaccagaa tgaccagcaa taccagtacc
    13501 gtattcatca ccgtcgcgtt gtagccgatg tagccatact gatagccaat ctgacctaaa
    13561 ccaccggcac cgactgcacc acccatcgcg gaataaccga ccagggtaat cagggtgata
    13621 gttgccgcat tcaccagacc cggcaacgct tccggtaaca gcactttacg gacgatctgc
    13681 attggcgtgg cacccattgc gcgggaagct tcaattaacc cggttgggat ctccagcaga
    13741 gcgttctcga ccatacgggc aataaacggt gctgcaccaa cggttaacgg aacaattgct
    13801 gcctgcaaac caatcgatgt accgacaata acgcgggtaa acggaatcat ccataccagc
    13861 aagataatga acgggatgga acggaaaatg ttcacaatcg cagaaacggt acgatacagc
    13921 ttcgcgttag caataatttg ccccggacgc gtgacataaa gcagaacgcc aaccggcaga
    13981 ccaatcacaa acccaaaaaa tccggatacg aaggtcattg ccagcgtttc ccatacgcca
    14041 cgaaccagca gccacatcat cggctcagac ataacccagt acctctactt ttacatggtg
    14101 ttcctgcagc caggcaatgg cggcttgcgt atcttgttgt gtgccgtgca tttcagtcag
    14161 catgatgccg aatttaacgc caccggcgta gtccatctgc gcgctaataa tgttgttgtt
    14221 gacgttgaaa cgacgcgcgg tttcagaaag cagtggggca tcgaccgatt gaccggtaaa
    14281 ctccagacgc agcatcggca cgcagtcagt aaatggctcc gcttgcagac gttcctggta
    14341 atcttccggg atatccagat gcagggtcga ctgaataaac ttctgcgcca gcggcgtttt
    14401 cggatgcgag aacacttcac ttaccgtgtc ctgctcgatc agttcgccat tgctgatgac
    14461 cgccacgcaa tcacaaatac gcttcacaac gtccatttcg tgggtgatca acagaatcgt
    14521 caaacccaga cggcggttaa tgtctttcag cagttcgaga atagaacgtg tcgttgccgg
    14581 gtccagcgcg ctggtggctt catcacacag caatactttg ggattgctgg ctaatgcacg
    14641 ggcaatcgcc acacgttgtt tctgcccacc ggaaagattc gacgggtagc tatcatgctt
    14701 atcgccaaga ccaaccaatg acagcaattc cgtcacgcga cgtttgatat cgtctttcgg
    14761 tgtgttgtcc agctccagcg gcagagccac gttgccaaaa acagtacgcg aagagagcag
    14821 gttaaaatgc tggaaaatca taccaatctg gcggcgagct ttggtcaact cggattctga
    14881 cagcgtggtc aattcctggc catcgaccag cacgctaccc tcggttgggc gctccagcag
    14941 gtttacacaa cgtataagcg tactcttacc cgcgcctgag gcaccgataa cgccataaat
    15001 ttgtccagct ggcacatgca ggctgacgtt gttcaacgcc tggatggtgc gggtgccctg
    15061 gtggaacact ttggtgatat tcgaaagttt tatcattgat tatttattat cgtcattaag
    15121 ttagtcgtag catctcgaat gcctgaaacg ggcaacgccg tcaatgaaat ggatgttaag
    15181 gcatccagac gtctaaatca atcaggttta tgcgaagaac actttcttgc aggtcgaaac
    15241 atgcgatact agcgtcccat gccttattaa ggagctataa aaggtggcga agagcgtacc
    15301 cgcaattttt cttgaccgtg atggcaccat taatgtcgat cacggctatg tccatgagat
    15361 cgacaacttt gaatttatcg acggtgttat tgacgccatg cgcgagctaa aaaaaatggg
    15421 ctttgcgctg gtggtagtaa ccaaccagtc tggcattgct cgcggtaaat ttaccgaagc
    15481 acagtttgaa acgctgaccg agtggatgga ctggtcgctg gcggaccgag atgtcgatct
    15541 ggatggtatc tattattgcc cgcatcatcc gcagggtagt gttgaagagt ttcgccaggt
    15601 ctgcgattgc cgcaaaccac atccggggat gcttttgtca gcacgcgatt atttgcatat
    15661 tgatatggcc gcttcttata tggtgggcga taaattagaa gatatgcagg cagcggttgc
    15721 ggcgaacgtg ggaacaaaag tgctggtgcg tacgggtaaa cctattacac ctgaagcaga
    15781 aaacgcggca gattgggtgt taaatagcct ggcagacctg ccgcaagcga taaaaaagca
    15841 gcaaaaaccg gcgcaatgat taaaagatga acggttgaaa taaaaatgca tttttccgct
    15901 tgtcttcctg agccgactcc ctataatgcg cctccatcga cacggcggat gtgaatcact
    15961 tcacacaaac agccggttcg gttgaagaga aaaaatcctg aaattcaggg ttgactctga
    16021 aagaggaaag tgtaatatac gccacctcgc gacagtgagc tgagagc
//
LOCUS       DADLSA010000090        15989 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.090, whole genome shotgun sequence.
ACCESSION   DADLSA010000090 DADLSA010000000
VERSION     DADLSA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.090"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..115)
                     /locus_tag="JN691_002761"
     CDS             complement(<1..115)
                     /locus_tag="JN691_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 domain-containing protein"
                     /protein_id="HAY7244169.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNGESALIIKMNDYGD"
     gene            complement(242..973)
                     /gene="rlmB"
                     /locus_tag="JN691_002762"
     CDS             complement(242..973)
                     /gene="rlmB"
                     /locus_tag="JN691_002762"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAY7244170.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     TGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1153..3594)
                     /gene="rnr"
                     /locus_tag="JN691_002763"
     CDS             complement(1153..3594)
                     /gene="rnr"
                     /locus_tag="JN691_002763"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAY7244171.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPNQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3633..4058)
                     /gene="nsrR"
                     /locus_tag="JN691_002764"
     CDS             complement(3633..4058)
                     /gene="nsrR"
                     /locus_tag="JN691_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAY7244172.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4263..5561)
                     /gene="purA"
                     /locus_tag="JN691_002765"
     CDS             complement(4263..5561)
                     /gene="purA"
                     /locus_tag="JN691_002765"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAY7244173.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5665..5862)
                     /locus_tag="JN691_002766"
     CDS             complement(5665..5862)
                     /locus_tag="JN691_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAY7244174.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5944..6948)
                     /gene="hflC"
                     /locus_tag="JN691_002767"
     CDS             complement(5944..6948)
                     /gene="hflC"
                     /locus_tag="JN691_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAY7244175.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWCISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6951..8210)
                     /gene="hflK"
                     /locus_tag="JN691_002768"
     CDS             complement(6951..8210)
                     /gene="hflK"
                     /locus_tag="JN691_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAY7244176.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8296..9576)
                     /gene="hflX"
                     /locus_tag="JN691_002769"
     CDS             complement(8296..9576)
                     /gene="hflX"
                     /locus_tag="JN691_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAY7244177.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9652..9960)
                     /gene="hfq"
                     /locus_tag="JN691_002770"
     CDS             complement(9652..9960)
                     /gene="hfq"
                     /locus_tag="JN691_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAY7244178.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10046..10996)
                     /gene="miaA"
                     /locus_tag="JN691_002771"
     CDS             complement(10046..10996)
                     /gene="miaA"
                     /locus_tag="JN691_002771"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAY7244179.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10989..12836)
                     /gene="mutL"
                     /locus_tag="JN691_002772"
     CDS             complement(10989..12836)
                     /gene="mutL"
                     /locus_tag="JN691_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAY7244180.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIAPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSLAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12846..14183)
                     /gene="amiB"
                     /locus_tag="JN691_002773"
     CDS             complement(12846..14183)
                     /gene="amiB"
                     /locus_tag="JN691_002773"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAY7244181.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRSEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQEQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14202..14663)
                     /gene="tsaE"
                     /locus_tag="JN691_002774"
     CDS             complement(14202..14663)
                     /gene="tsaE"
                     /locus_tag="JN691_002774"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAY7244182.1"
                     /translation="MMNRVIPLPDEQVTLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14635..>15989)
                     /gene="nnr"
                     /locus_tag="JN691_002775"
     CDS             complement(14635..>15989)
                     /gene="nnr"
                     /locus_tag="JN691_002775"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY7244183.1"
                     /translation="LCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLPEEAALAR
                     EAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSHPAPIVAV
                     DIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLGLDSWLAG
                     QETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEAALRAGAG
                     LVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQEWGKKALQ
                     KVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIESDRLHCAK
                     RLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGALLGQKLS
                     PYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHDESSNSAP
                     "
BASE COUNT         3597 a         4603 c         3981 g         3808 t
ORIGIN      
        1 aatcgccata atcattcatt ttaataatta atgcgctttc gccatttgtt acatcaatat
       61 tttgttcagg tacagtttgc agcgccgtcg ccagcgccaa cggattccat gtcatatatt
      121 attccatata gattaagttt aaatattaat aaaatgaata tttgcaatac gtaattatct
      181 taccagctat agacaaaaaa aaaccatcca aatctggatg gcttttcata attcagagga
      241 actagctgcg ctgacgaacc gcttcaaata agcaaattcc ggttgcaacc gaaacgttca
      301 gggaagaaac acttcctgtc attgggatgc tgatcaactc atcgcaatgt tcacgggtca
      361 ggcgacgcat accttcacct tccgcgccca tcaccagcgc caggcgtccg gtcattttgc
      421 tctgatagag tgtatgatcc gcctcgcctg ccgtaccgac gatccagata ttctcttcct
      481 gcaacatacg catggtgcgc gcaaggttag tcacccgaat cagcggaacg ctttctgccg
      541 cgccgcaggc tacttttttc gccgtagcgt tgagctgtgc ggagcgatct ttcggcacaa
      601 tcaccgcatg aacgcctgcg gcgtccgcac tacgcaggca cgcgccgagg ttgtgcggat
      661 cggttacgcc gtcgaggatc agcaggaacg gttgatcgag cgaagcgatc agatccggca
      721 gatcgttttc ctgatactga cgtcctggct tcacgcgggc gataatgccc tgatgcacgg
      781 caccgtcgct tttctcgtcg agatattggc ggtttgccaa ctggataacc acgccctgag
      841 actcaagggc gtgaatcagc ggtaacagac gtttatcctc acggcctttt aaaataaaga
      901 cttcctgaaa acgttcaggg gcgcgctcca gcagggcctg cactgcgtgg atgccgtaaa
      961 tcatttcgct cattaatgta ctcgttgttt acgttttgcc tgatgcacta cgtttatccg
     1021 gcctacatga tctctgcaat atattgaatt tgcgtgatta tgtaggccgg ataaggcgtt
     1081 cacgccgcat ccggcatgaa caaagcgcat tttgtcagca atctaaccct cttcttttaa
     1141 agagggtatt gatcactctg ccactttttt cttcgccgca cgcttcgctt tggtcgctgc
     1201 ggctattttc tgcgttttcg ccgatggctt tttcgctttt ctcgcgtctt tcttcgccgc
     1261 tttcggcttc gtttttttct caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt
     1321 accgacctga cgacgcttgc cgcctttttt gcctgcatcg ccttttttcg ctttctcgcg
     1381 cgccgtttta ccgacgttgc gcggtgcgcg ttcactggag atcagactaa agtcgatttt
     1441 gcgctcgtcc atattaaccg cttcgacgcg aacttccacg cgatcgccca ggcgataagt
     1501 ctggccgctg gattccccca tcaggcgttg ccctacctgg tcaaagcgat agtagtcatt
     1561 gtccagcgaa gagacatgga ccagaccatc aatgaacaag tcgtccagac ggacgaagaa
     1621 gccaaagcca gtgacgctgg aaattacgcc tttaaagacg ttacctacct ggtcgagcat
     1681 gaagtcacac ttcagccagt cagcgacatc gcgcgttgct tcgtcggcac gacgttccgc
     1741 catcgaacag tgctgaccca gttgcaacat ctcttccatc gaataatggt agccgccggt
     1801 ttcagtggtg ttgccctgat gcccctgctc tttcgccagc agatatttaa tggcgcggtg
     1861 cagcgtcagg tcaggataac gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc
     1921 cagaccgaag tgaccacggt tttctggatc gtaaatcgcc tgtttcatcg agcgtagcag
     1981 catggtttgc agcatttctg catcaggacg gtcggcaacc gactccagca gttccgcgta
     2041 gtcacgcggt tccggcttgt taccacccgg cagctccagc cccagctccg ccagcactga
     2101 acggaaagag gtaatcgctt cggtgctcgg cttgtcgtga atacggaaca gtgccggttc
     2161 tttcgctttc tcaacgaaac gcgccgccga gatattcgcc agaatcatgc actcttcaat
     2221 taatttgtgc gcgtcgttac gctgggtctg ttcgatacgt tcaatacggc gttcagcgtt
     2281 gaaaatgaac ttcgcttctt cgctctcaaa tgagatccca ccgcgttctt cacgggcttt
     2341 atccagcact ttatagaggt tatgcaactc ttcgagatgc ttaaccagcg gggcgtactg
     2401 ctcgcgcaga tcctgatcgc cctgcagaat atgccagact ttggtgtagg tcagacgcgc
     2461 gtgagagctc atcaccgctt cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac
     2521 cgtcatctcg cacaccatac acaggcggtc tacctgcggg ttgagcgaac acaggccgtt
     2581 agagagcact tccggcagca tcgggataac ctgcgaaggg aagtacaccg acgtgccacg
     2641 gttacgcgct tctctgtcca gcggcgttgg cggacgcaca tagtagctga cgtcggcaat
     2701 cgcgacccat aaacgccagc cgccgccgcg ttttttctcg cagtaaactg catcgtcaaa
     2761 gtcacgggcg tcttcgccat caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc
     2821 cgcttttgct tcttccggca cttcttcttt cagccccgca acctgttgct caacagcctg
     2881 cggccagata tacggaattt catgggtacg cagagcgata tcaaccgcca tgccggtgcc
     2941 catattgtcg cccagcactt cgacgatttt acccaccgct ttggtgcggc gagtcggacg
     3001 ctgagtcagt tcgactacga ccacaaagcc catccgcgcg cccatgatct gattgggcgg
     3061 gattaagata tcgaagctca gacggctatc gtcaggaacc acaaagccga cgcccgcttc
     3121 ggtaaagtag cgaccaacaa tctggctggt ttttggcacc agtacgcgga caatacgcgc
     3181 ttcacgacga cctttacggt cagcacccag cggctgcgcc agcacctgat cgccatgaat
     3241 gcaggttttc atctgctcgc tggagagata caaatcatct ttacgccctt caacccgcag
     3301 aaagccgtag ccatcacggt ggccaataac ggtacctttc accaggtcga ggcgttccgg
     3361 cagcgcatag cactgacgac gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag
     3421 gcggcgacgc aggccttcaa gctgctcttc gccttcaatg tgcagttcta ccgccagctc
     3481 atcacggctg gccggttttt cacgtttggt taaatgttcg aggataaatt cccgactagg
     3541 gatgggattc gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt
     3601 tcctccgttg tcatctctga tgaagatttt cgtcactcca ccagcaataa tttataaagc
     3661 ggttgattct cttcaaccaa atcggcaagc gtgtagttat ccagttccgt aagaaaactt
     3721 tgcacggcct tagaaagcgc ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca
     3781 ctgctgcaat tcaccagcga taagggctcc agctcgcgca ccacatcacc aatacgtatc
     3841 gcactcgccg gtttacccag gcgaatgccg ccattttttc cacgaacagc agtcacgtag
     3901 ccggcacgac taagttgatt gattattttg accatatgat tacgggagac gccgtagacg
     3961 tcagtcactt cagaaatact ggtcatccgc ccttctggca atgacgccat gtagatcagc
     4021 gcacgtaatc cgtaatcagt gaaactcgtt aactgcacat caacctcaaa agggaaatcg
     4081 ggaaaaaata catttatatt gatgataaac cagccacaag ctgtgtcgct aatttatttc
     4141 agttcgggga aggaaaaaag cgaggattta acactgtgtc ggatagcgta atggcttaac
     4201 cgacacagca atgtatcaca ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag
     4261 aattacgcgt cgaacgggtc gcgcagaatc atggtttcag tacgatccgg accggtagag
     4321 atgatatcga tcggcacacc ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc
     4381 tgcggcaggc cgctacgatc tttcacgccg aaagtggatt cagaccagcc cggcatggtt
     4441 tcgtaaatcg gctctacacc tttccagtcg tcagctgcca gcggagtggt agtcacttcg
     4501 cgaccatccg gcatacggta agccacgcag agtttaacct ctttcaggcc atccagaacg
     4561 tccagtttag tcaggcagaa gccagacagg gagttcagct gtaccgcacg acgaacggca
     4621 acggtgtcca gccagccggt acgacgacga cgacccgtag ttgcaccgaa ttcgttaccc
     4681 tgcttgcaga ggaactcgcc agtttcatca aacagttcgg ccgggaaagg acctgcacct
     4741 acacgagttg agtaagcttt gaggataccc agaacgtaat caacataacg cgggcccagg
     4801 ccggaaccgg tcgccacgcc accagcagtg gtgttggaag aagttacgta cggataagta
     4861 ccgtggtcga tatccagcag cgtaccctgc gcaccttcga acatgacgaa atcgccacgc
     4921 tgacgcgcct ggtcgagcag gtcagaaacg tcaaccacca tagaagtcag gatgtcggca
     4981 acagccatcg tatcatccag aactttctgg taatcaaccg cttcagcttt gtagtagtta
     5041 accaactgga agttgtgata ttccatcact tctttcagtt tttcagcgaa ggtttctttg
     5101 tcgaaaaggt cgccaacacg cagaccgcga cgtgccactt tatcttcata agcaggcccg
     5161 ataccacgac cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc
     5221 agcgcaacgt gataatcaag gatcagcgga catgcttcag acagcagcag acgctcacga
     5281 acggggatgc cacggtcttc cagttctttc atctctttca tcagcgcggc cggagacagc
     5341 acaacaccgt taccgatgat gctggttaca ttctcgcgga gaatacctga tggaataaga
     5401 tggagaacgg ttttttcacc gttgattacg agagtatggc ctgcgttgtg accgccctgg
     5461 tagcgtacaa catatttagc ccgttcagtc agaagatcga cgatcttacc tttaccttcg
     5521 tcaccccatt gggtgcccag tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg
     5581 cttaaaaatg gattttacca tcgctttttc agagttacag cactttttgc actcaaaaat
     5641 gacctcgatc agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt agtagaccac
     5701 aacgcccgca accacaagtc caccgccaaa acgacgtaaa atattatcgg gcaaattggt
     5761 catcgcagag atcatcttct tccatgcctt cgggtaaagc atcggcccta aaccttccag
     5821 taccaaaacc agggcaagcg ccagccagat tgtcgaattc attcattatc cttatagaaa
     5881 aagaaaacca ccgacatccc tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt
     5941 atattaacgc gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc tatccgggct
     6001 catgaccatc acgtcctgat tgccagagaa gctgttctca taagcacgca ggctacggat
     6061 gaatgcgtag aagtccggat ctttactgaa tgcatcagca aacagtttgg ctgcttcggc
     6121 atcgccttca ccacgcataa tgcggccctg acgctcagct tctgccagcg ttctggtcac
     6181 ttcatagtcg gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg
     6241 acgcgctacc gcttcacgct cggcgcgcat acggttgtag atcgcttcag acacttcggt
     6301 cggcaggttg atctgcttga tacgcacatc gacaacttca atacccagcg ccgccatact
     6361 gttcgggttg atgaccggaa ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc
     6421 ggcaatggcg ttatctgctg ccggggtagt aacttcatct tctgtacccg cagaaccgga
     6481 gttcagcgcg tcacgtactt cgagggtcag acgaccacgg gaatcggtga cgatatcttt
     6541 cacgtccagg cgaccaattt cagaacgcag acggtcagag aacttacgtt tcaacagcac
     6601 ttccgcttgc gaaatgtcgc caccacccgt tgccaggtag taacggctga aatcgctgat
     6661 gcaccatttg atgtaagagt cgacgatcag gtctttcttc tctttggtca caaagcggtc
     6721 ggcctggttg tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg
     6781 tatcttgaaa tgcagaccag gctcataaac caaaggtttg ttgtcatcgt cacgcagtac
     6841 cttaccaaaa cgcagcgtaa taccgcgctc accttctttg acgacaaaga cagacatgta
     6901 aagcactacc agcacgatga tgataatcgc gataactgac ttacgcatcg ttattccccc
     6961 tgacgctggt agtcgttacg ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga
     7021 ctggtggacg acgtgttgct tgctccactg gttgtggagg aagaggctgg cggcagacgc
     7081 agcagattgc tggcaccgtt atcgctcttc gccgcagggg cgttaccacc tttcagcatc
     7141 tggtctaacg gcagaaccat caggttgcca cctttatcgt taaccagcac tttgcgggtg
     7201 ttacccaaca ctttttccat cgtctcgata tacagacgct cgcgagtaat ttccggcgcg
     7261 gctttatatt ccggcagaag tttagcaaag cgcgccactt caccctgagc ttccaggatg
     7321 gtctgggcct tgtacgcacg cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc
     7381 ggctgaactt cgttggtata cgcttctgct tcacgaatgt attgctgttc gttttcacgc
     7441 gcggcaatcg catcgtcaaa cgccgctttt acttcttccg gcggacgagc agcctggaag
     7501 ttgacgtcca gcagcgtgat acccatgtca tacggacgaa tcgtctcttc cagttcgcgc
     7561 tgagtatcgc tacgaatcac ggtacgacct tccgtcagaa tgcggtccat ggtgtattta
     7621 ccgataactc cacgcagggc gctgtcggta gcctgacgca ggctgtcatc cgggctggtc
     7681 acgctataca gatatttttc cggattggtg acgcggtact gcacgttcat ctcaacgcgc
     7741 actacgttct cgtccgacgt cagcatcaca ccagaagcgg ccagttcacg cacggcttcc
     7801 acgttcaccg gtttgacttc gtcgataaac gtcggtttcc agttcagacc cggctcaacc
     7861 agatggctga atttaccaaa gcgtgttacc acgccgcgtt cggcttcttt aatggtatag
     7921 aaaccactgg ccgcccagat aatgacaatc gctgccgctg cgatggtaac gacacgaccg
     7981 ccaagctgcg ggcgcgggcc ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg
     8041 ccgcccagac caccgagctt tttgctcagt ttgcggaaga tatcatctaa atcaggtggc
     8101 ccttgatcgc gaccgccttt gtttccattt ccctcagagt tgccgccagg tttgctgctt
     8161 ccccacgggt cgcggtcttg tccgttatta ccgggctgat tccacgccat gtttgtgctc
     8221 catatttgtt atgcggtgat ccctgttgtc tttcgacgcc agaggatatg actccacgct
     8281 tcagacgcta cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg
     8341 acgccagtca acgatcggca tacgaacttg cagacttacg ctgccgtcct cctccatcca
     8401 ctctttttct attgcctgaa gctgataaaa acgacttctc agacgccctt cctgcggtgg
     8461 cagacgcaat gtatgctgcg ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag
     8521 ctgtggtatc cccgctccgg tctgtgcgga aagccagaca cggatcggtt tgttctcttc
     8581 gtcccgatca atacgcggtt cgaaatcttc cagcatatcg atcttgttca tcaccagcag
     8641 ggttgggatc tcgtgagcgt cgatctcttc aagaaccgta ttcaccgctt cgatgttttc
     8701 ttgtacacgc acatccgccg catcaatgac gtgcagcagt aatgtggctt gccgcgtctc
     8761 ttgtaacgtg gctttaaatg ccgccaccag atcgtgcggc aggtggcgaa taaaccctac
     8821 ggtatctgca agtacggttt caccgacatc tgcaacgtca atacgccgca acgtcgggtc
     8881 gagggtggca aacaactggt ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag
     8941 ggtagattta ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt cggctttgat
     9001 acgcgattgc cgcccctgct cacgctgctt ttcaactctt tccaggcgcg actgtatctg
     9061 cacgatgcga ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac ccggaccacg
     9121 caaacctatc ccgcctttct gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc
     9181 cagatggcgc agctgcgcca gctcaacctg caacttaccc tcatgggtac gcgcacgttg
     9241 ggcgaaaata tctaaaataa ggccggtgcg gtcgataaca cgacactcgc acaaacgctc
     9301 caggttacgc tcttgcgccg ggctcagggc atggtcaaaa agaacgaccg aagcacccgt
     9361 cgctttgaca gcttccgcaa tttcaactgc tttaccttca cctacaaaat actttgggtg
     9421 cggcgcttta cggctaccgg taatcacctg caatgcttcg acaccggcgg aagagaccag
     9481 agattcaaac tcctggaggt cttccatatc tttgtcttgc gtaaaataga tgtgtaccag
     9541 taccgcctgc tcaccagcat cataacggtc aaacaagcgt ataaccctct aaatagatca
     9601 gcggggaacg caggatcgct ggctccccgt gtaaaaaaac agcccgaaac cttattcggt
     9661 ttcttcgctg tcctgttgcg cggaagtatt ctgcgcgctg ctaccatgat ggtagttact
     9721 gctggtaccg ccaccggcgt tgttactgtg atgagaaacc gggcgagacg ggacaacagt
     9781 agaaatcgcg tgcttgtaaa ccatctggct gaccgtgttt ttcaacagga tcacgaactg
     9841 atcaaaagac tcgatttgcc cttgcagctt aataccattc accaaataaa tagaaactgg
     9901 aacacgttcc cgacgcagtg cgttcaggaa cggatcttgt aaagattgcc ccttagccat
     9961 tctctctttt ccttatatgc ttatttgtac tttgaacctt tcgattctga aaaaattgcg
    10021 cacgatacgt ctcaattgta cacattcagc ctgcgatagc accaacaacc tgtaatactt
    10081 cgtcacgcgc ctgttctggt ttttcactgt caagccagtg aaccccttcc caaccacgca
    10141 gccaggttat ctgccgcttc gccaactgtc tcgtggcgca aacacctcga taaaccattt
    10201 catcgtatga gatttcgcct tcaaggtaag accacatctg gcgataaccc acgcaacgaa
    10261 tggaaggcaa gtccgtatgc aaatctcctc gggcaaaaag cgcccggact tctgcttcaa
    10321 aacctgaagc caacatctga tgaaaacgct gctcaatgcg ttgatggagc agttcacggc
    10381 tcgccggggc gatggcgaac tgatgcacct gatacggtag agcgtctcct gacgtttgcg
    10441 tcagttccgt taaagtttta cccgaaatga aaaaaacttc cagtgcccgg gaaagccttt
    10501 gtggatcatt tggatgaatc cttgctgccg caaccggatc tacctcctga agttgacgat
    10561 gcaatgactc ccaaccttgc tctgccgcct gttgctcaat tctggcccgc acttccgggt
    10621 ctgccgacgg tagcggcgac aacccttcca gcaatgcctt gaaatacaac atcgtaccgc
    10681 ccactaacag tgggatccgc cccgccgcgg tgatatcggc catttccgcc agcgcatcgc
    10741 ggcgaaaatc agcagccgag taagcctgcg acggatcgcg aatatccagc aatcggtgcg
    10801 gcgcggcgag taactcttca gcgttcggct tcgccgtccc gatatccatc cctttgtaaa
    10861 taagggcaga atcaacgctt atcaactcta ctggtaaaat tttacgcagc tcaatggcta
    10921 acgccgtttt accggaggcc gtcggcccca tcaaaaaaat cgccttaggc aggctcgcct
    10981 tactgatatc actcatcttt cagggctttt atcgccggat gtaaatcaac agattgtaac
    11041 agaccacccg gcggcgtttt cacaagttgc ggacataacc gttccacgtc cgccagcagg
    11101 gttatggcct gtgccattga ccactgcgca tgttcgctca tcagatttcg tgcaatccac
    11161 tgcgcaatat tgccaggttc gaatacggac tgcttcgcca ggtagcctat cagttcagga
    11221 atcaagattt gtaaattttg ttggcgtaag ggtaaaggca cggccctgat ggtcacatgc
    11281 tgtgcatctg actggaaatc aatacccaat tccgctaggg cagactgcgc tttttctaat
    11341 gccgattttt cttcgccaga aacttttagc cgcaacggaa tcagcagcgg ctgggcgcaa
    11401 acgggcgctt cacccggcgt caattgtgcc tgacgcagcc aacgttctgc cactggcaag
    11461 gataaaagtg aaatgttgcc atcgcgctcc agcaacgcac agtcggaatg gacgatagtc
    11521 agtacccgac caaaactctg actgttcgcc gcaagtgcag gttcctgcgg ttccggcgct
    11581 tttggttttt gcatcggcgc gggcgtttgc aaaagctggc gatacacttc accttgctgt
    11641 ttctggtagc ctggctgcgc attcggccag ggggcagccg gacgactgcc tgaggcgggc
    11701 gcaagagagt agcgcggagc taccggctca cgagctgccg gttctgcaaa gtgattgcgc
    11761 cccgccgcca cgcggttttc cgggatggca cgcggtgcgg gttgtggttc atcgtccagc
    11821 ggtagcggcg tttccagttg ctgttgtagc acgctcagca cgccctgata gataaaatca
    11881 tgcaccagac gcgactgatg gaaacgcact tcgtgtttgg cggggtgtac gttgacgtcc
    11941 acctgatgcg gggcgatctc cagatacaac acaaatgccg gttgctgatc ggcccccagt
    12001 ttgtcttcgc aggcctggcg gatcgcatga ttgatcaggc gatcgcgcat catgcgacca
    12061 ttcacgtagc aatactgaat ttccgccagt gcgggcgtag tgtgatttgg atcggccacc
    12121 cagccgcgca gggtgagatc gccgtgttgc cattcaatcg ccagcgcttg ttcgagaaaa
    12181 gcggtgccgc aaatcgcgcc taagcgccgt tctttttgcc cgccttccgg cactgcgcgg
    12241 tactgacgca caattttacc gttatgcgac aggttgatcg tgacgtcgaa acgcgccagc
    12301 gcaatgcggc ggatgatttc atcaatatgg ctaaattcgg ttttctcggt gcgcaggaat
    12361 ttgcgccgcg ccggggtgtt gtagaacaga tccagcacct ccagcgtcgt tcccaccgga
    12421 tgcgccgccg gtttaaccgt cacgtccatg tcgcgtcctt cggcataggc ctgccaggct
    12481 tcctgctgtt ctgcggtgcg tgaagtgagc gtcaggcggg aaaccgaact gatactcgcc
    12541 agcgcctcac cgcgaaagcc caggctgata atggcttcga gatcgtccag agaggcgatt
    12601 ttactggtgg catgacgagc cagcgccagc gccagctcat cttttttgat accgcagccg
    12661 ttatcacgaa tgcggataag tttcgcccca ccgcgttcga tatcaatatc gatacgcgtc
    12721 gcacctgcat cgaggctgtt ttccactagt tctttgacta ccgacgcagg tcgctcgacc
    12781 acctcacctg cggcaatctg gttcgccagt tgtggcggta agacctgaat tggcatcaat
    12841 cgtccttagt ttggcagcgt gcgatctggc gtcgtcaccg tactggcagt ttgtgccgtt
    12901 gcaccctgcg gcgcagattg catcggatgc gcaaggaagt aattgcgcag gcctttgtaa
    12961 atggcttctg ccagctgttc ttggtaatcg tcgctcgcca gcaaacgttc ttcgctgttg
    13021 ttgctgataa aaccggtttc gaccagtact gatgggatat ccggcgagcg cagaacgcca
    13081 aggctggcgt gttctgatcg acgtttatgt atttcgccaa tgcgttgcaa ctgactgatc
    13141 atactggtcg ctacatcata ccctacccgc tgggaatgac cgaactgtaa atccagcacc
    13201 gcctggctta aataggggtc agactgactg ttcgccagca catcacccgc cccacccagc
    13261 agctccgact gtttctcgtg ctgctccagc cagctggcca tttcactgtt ggcgcgacgg
    13321 ttagagagca cccatacgga agcgccagtc gcactgcggt tcggtgcggc atcagcgtga
    13381 atcgacacga ggaaattggc gttttgctta cgtgccacat cactgcgccc catcaccgag
    13441 ataaagtaat ccccgtcacg ggttaaaacg cctttaaaca tcggatcgtc attgagcaaa
    13501 gtacgcaatt tacgcgcgat ggcgatggtg acatttttct cccgcgtacc accggggccg
    13561 atagcgccag ggtcctgacc gccgtgtccg gcatcaatag cgatgataat tttatcgcca
    13621 gtgttagccg tcgcgcgcgc tgccggacgc gttaccgtat tactgctgat aacacccgta
    13681 gtgcggttac tttccgtttt aaacggattg cgcgccggtt cgctgacgcg cggtgcgaca
    13741 accgcaggcg tttcaacgcg tttcgcaacc acgggcggcg gaggaggcgg tggcggcaca
    13801 tcggcgttaa tcgtaaagac gacagtgtaa ttgctgccat tctgccgctt caccgcttcg
    13861 gttttaccgt tttcggtaag atcgaccacc agccgtagcg tttgtgcatc tttaggcgtt
    13921 ccagagcgaa tcgccttcac cagattattg ccgctgaaca acaacggcag tccctgaatc
    13981 acgcccgttt gtttgatatc gagcgccacg gtgcgtttgc tttgatggct aaacgcataa
    14041 tcaggatcgc caataaaact caacgttatc cgcgcctgtt gattaccgtt agaaacctga
    14101 atatcagaga gcgtcgcggc acccaccggc gtgcacagca gcagcagcgt cgctaccaac
    14161 caatttctga tgcgatacat catcccgcca cctttcaaag gttaaccggc taaacgcgcc
    14221 agcaacaatt cacccgcaga ggaaaccgca ctcacgcgcg cctcacggcc ttgtgcctga
    14281 taatcaatgt gtatttcgac atccgggtca ggaagaacac ctgtaccttg ttgtggccac
    14341 tccaccaggc agatggcatc gttggcaaaa taatcgcgga tccccataaa ctccagctcc
    14401 tcgggatcgg caaggcggta caaatcaaag tgatagacca ttaagttgtc gagcgtatag
    14461 ggttcgacca gcgtataagt ggggcttttg acattaccct gatgacccag agcctgtaaa
    14521 aagccccggc taaaggtggt tttacctgcg cctaaatcgc catacagata gattacggtt
    14581 gcgccatcgc aggctttcgc tacccgctcg cccaggtcta atgttacctg ctcatcaggg
    14641 agcggaatta ctcgattcat catggttttt atcagtcact tccgggttaa caatacgctg
    14701 tagcgtggaa aagagatcgg ttgccagcat cccgcgcgtt ccaaaacgcg ccgccagtac
    14761 gtcagctgcc gcaccgtgcg cgacacagcc tgcacaggct gcatcatacg gcgacagttt
    14821 ttgcccaagc aatgcgccaa taataccaga gagcacatcg cccatgccgc cgctcgccat
    14881 gcctgcattt ccggcatcaa taatgcctaa agcgtcagga tgggcggcga ccacggttcc
    14941 ggcacctttc agcaccgcta cgccgccata acgttgtacc agacgtttgg cgcaatgtaa
    15001 gcggtcactt tcaatttcag cgacggaaca gcctaacaac cgtgcggcct cgccaggatg
    15061 cggcgtgatc acgcgattgt gacgcttatc gggattgatt gccagcaggt tcaatgcatc
    15121 ggcatcccac aacatcggtt tgcgaaaatt ctcaactttt tgcagggctt ttttccccca
    15181 ctcttgctgg cccagaccgg gaccaatcac caccacatcg gcccattcca ggctttcggt
    15241 aagagagtcc atagtcagtt catgcaccat caattctggt cgtgcagtca gcagcggcgc
    15301 aatgttctca ctgcgggtca gtactcggac taaaccagca ccagcacgta gcgccgcttc
    15361 ccccgtcata cgaatagccc ccgccgtgcc gtgatcgcca ccgataatca ccagccgccc
    15421 gtgatcgcct ttatgcgaag tcgggcgacg cggtttcagc cagtgagaaa gttgttctgc
    15481 cgaaaaccgc tgaattttcg tctcctgacc tgccagccag ctatccagcc ccagtgagtc
    15541 aaaatgcagt tgtcctgtaa catcccgcgc ttttccagtg agcaagccta gtttcagcgc
    15601 aataaaagtg atggtgtgat cggcgttgat cactgcgcct ggcgtagcgc cggtttcagc
    15661 cagcaggccg gaagggatat caaccgccac aatcggcgca ggatgggaat tagcgtggtc
    15721 gattaactgg ctaatggatt cgcggggcgc ttgctgcaag ccggtaccga gcagcgcatc
    15781 aacaatcaga tctaccgatt cgggccagac aatattcgaa gcatggatct cgccacccgc
    15841 gtttaaccat gcttcgcgtg ccagcgcggc ctcttccggc aacggtttgt cgctctcctg
    15901 ggccaacaac gtgacctcaa tgccgaccgc tttggccagt cgcgcgacca cgtagccatc
    15961 gccgccgtta ttaccatgac cgcacagca
//
LOCUS       DADLSA010000091        15647 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.091, whole genome shotgun sequence.
ACCESSION   DADLSA010000091 DADLSA010000000
VERSION     DADLSA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.091"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(94..201)
                     /gene="rprA"
                     /locus_tag="JN691_002776"
     ncRNA           complement(94..201)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="JN691_002776"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(387..1499)
                     /gene="ydiK"
                     /locus_tag="JN691_002777"
     CDS             complement(387..1499)
                     /gene="ydiK"
                     /locus_tag="JN691_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAY7244184.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1888..4944
                     /locus_tag="JN691_002778"
     CDS             1888..4944
                     /locus_tag="JN691_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY7244185.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGIVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4941..5351
                     /gene="menI"
                     /locus_tag="JN691_002779"
     CDS             4941..5351
                     /gene="menI"
                     /locus_tag="JN691_002779"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAY7244186.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5451..5639
                     /locus_tag="JN691_002780"
     CDS             5451..5639
                     /locus_tag="JN691_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244187.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            6187..6555
                     /gene="sufA"
                     /locus_tag="JN691_002781"
     CDS             6187..6555
                     /gene="sufA"
                     /locus_tag="JN691_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAY7244188.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6564..8051
                     /gene="sufB"
                     /locus_tag="JN691_002782"
     CDS             6564..8051
                     /gene="sufB"
                     /locus_tag="JN691_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAY7244189.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKSVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEQSVG"
     gene            8061..8807
                     /gene="sufC"
                     /locus_tag="JN691_002783"
     CDS             8061..8807
                     /gene="sufC"
                     /locus_tag="JN691_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAY7244190.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8782..10053
                     /gene="sufD"
                     /locus_tag="JN691_002784"
     CDS             8782..10053
                     /gene="sufD"
                     /locus_tag="JN691_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAY7244191.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTESLRDEGLKQQVLARIGQRLPGGAR"
     gene            10050..11270
                     /gene="sufS"
                     /locus_tag="JN691_002785"
     CDS             10050..11270
                     /gene="sufS"
                     /locus_tag="JN691_002785"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAY7244192.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            11283..11699
                     /gene="sufE"
                     /locus_tag="JN691_002786"
     CDS             11283..11699
                     /gene="sufE"
                     /locus_tag="JN691_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAY7244193.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11848..12852
                     /gene="ldtE"
                     /locus_tag="JN691_002787"
     CDS             11848..12852
                     /gene="ldtE"
                     /locus_tag="JN691_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAY7244194.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12916..13152)
                     /locus_tag="JN691_002788"
     CDS             complement(12916..13152)
                     /locus_tag="JN691_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAY7244195.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13463..14875)
                     /gene="pykF"
                     /locus_tag="JN691_002789"
     CDS             complement(13463..14875)
                     /gene="pykF"
                     /locus_tag="JN691_002789"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HAY7244196.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     NATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            15432..15641
                     /gene="fumD"
                     /locus_tag="JN691_002790"
     CDS             15432..15641
                     /gene="fumD"
                     /locus_tag="JN691_002790"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HAY7244197.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         3971 a         3769 c         4145 g         3762 t
ORIGIN      
        1 attatcagcc tgctgacggc ttgaagagag tcacagtatc ttgtgcaaca ttattggaaa
       61 gagtgagggg cgaggtagcg aagcggaaaa atgttaaaaa aaagcccatc gtgggagatg
      121 ggcaaagact acacacagca attcgttgtt tcactcaggg gatttccatg cttataaatc
      181 aatatgttga tttataaccg tgagctaata gtaggcatac ggactttttg cgtcgatcag
      241 attcgtctca ttagtttaaa aactgtaaag aatttgcgac aggtactacc agctgaatgg
      301 cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcggtg attaacaatc
      361 aggcggcaga gccgcctgat gagaaattac ttattcggtt tctcgatttc gccaagttct
      421 tcgagaattt cttccggttg gtccgtcggc ggcgggactt cttccaccca cgcggcaaac
      481 agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat cccgaaagca
      541 atcaaaccac caataacgcc agagaggatc aggatcagcg gtaaatcggc acccatgcga
      601 attaacattg ggcggatgac gttatccagc gtgccaacca caccgctcca cactaacaat
      661 accgttcccc aggtggtatc gccggtccag tagagccaga taatcgccgg aatcagtacc
      721 ggcaacgggc caagctggac aaggcaggag aggatcatta acaccgttag caacgttgca
      781 taaggtacgc cggatacggc gaggccgata ccgccaagca ctgcctgtac taacgccgtc
      841 accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagcac ggcggcatct
      901 ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac ctgttcaccg
      961 cgccagtaca gcagggcact gaagagaagc atcagcgcac aatgcaccat aaagcgcccg
     1021 atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg gcggactttc
     1081 gccatgatcg ccgtaccccc catatccagc aagttgtgcc agcctgcata cagcttcgcg
     1141 ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc gccgctggaa
     1201 atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa agcaataggg
     1261 atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga gcggcggcca
     1321 aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac cgtaccggcc
     1381 catgcaaagc cgagaataaa gggttgaaca atccacagac atgccacaat catgatggct
     1441 aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac atttaccata
     1501 actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac gcgaaactca
     1561 cctgcataat gatgagcaat ttcagcggtt tttaacaggc cgattctgcc cttaattctg
     1621 atgggcgtgt tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa cgatttactc
     1681 gtcgtatttc aagttgcatg tgctgcggct acgttcgttc accccagtca cttactattg
     1741 taagctcctg gagttcactc gcttgcagcc ttcctgcaac tcgaaatcca tagagtaaat
     1801 aaacatcacc tttcagtgcg ctattgcagc cgcctgaaag aatagtgtat cgacctacat
     1861 cacagaccgc aggaaagggt caatataatg attccacaga tttcccaggc acccggcgtc
     1921 gttcaactgg tgcttaattt tttgcaagag ctggagcaac aaggttttac cggcgatgcg
     1981 gcgacaagtt atgccgatcg tctgacaatg tcgaccgaca acagtattta ccaacttctc
     2041 cccgatgcgg tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc ccgtcttgcc
     2101 gcgcaggaac gctattcatc gctgatcttt accccccgcg gcggcggcac cggcactaac
     2161 ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc gccatatgaa ccgcatcatc
     2221 gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa agaccaactc
     2281 aatcagtacc tgaaaccgtt cggctacttt tttgcgccgg aactttcgac cagcaaccgg
     2341 gcaacgctcg gtgggatgat caataccgat gcatccggtc agggatcgct ggtctatggc
     2401 aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt tggggggcga tattctcgat
     2461 acgcaacctt tacccgtcga gctggcggaa acgctgggta aatccaatac cacaatcggg
     2521 cgaatttata acacggttta tcaacgttgc cgtcagcaac gccagttaat catcgacaac
     2581 ttccccaaac ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt taacgatgag
     2641 atgaccgagt tcgacctgac gcgcattctg acgggttcag aagggacgct ggcctttatt
     2701 accgaagcgc ggctggatat tacgcgcttg cctaaagtgc gccgtctggt gaatgtcaaa
     2761 tatgactctt ttgactctgc gctgcgtaac gcgccgttta tggttgaggc gcgggcgctt
     2821 tcggtagaga cggtggactc aaaagtgctg aatctggcgc gggaagatat tgtctggcat
     2881 tccgtcagcg agttgattac cgatgtgcct gacaaagaga tgctcggact gaacattgtg
     2941 gaatttgctg gtgatgatga ggcgctgatt gacgagcgag ttaatgcact ctgtgtgcgg
     3001 cttgatgagc tgattgccag ccaacaggca ggtgtgattg gctggcaggt gtgccgcgag
     3061 ctggcggggg ttgaacgtat ctatgcgatg cgcaaaaaag ccgttggtct gcttggcaat
     3121 gccaaaggtg ccgctaagcc aattccgttt gctgaggata cctgcgtacc gccggaacat
     3181 ctggccgatt atattgctga atttcgcgcg ctgctcgaca gccacggctt aagctacggt
     3241 atgttcggtc acgtcgacgc aggtgtcttg cacgtccgtc cggcactgga tatgtgcgat
     3301 ccgcaacaag agattttgat gaagcaaatc tctgatgacg tggtggcgct gactgcgaaa
     3361 tacggtggtt tgttgtgggg cgagcacggc aaaggttttc gcgctgaata cagcccggcg
     3421 tttttcggtg aggaactttt tgcagaactg cgcaaagtga aagcggcatt tgacccgcat
     3481 aaccgactca acccagggaa gatttgcccg ccagaaggtc tcgatgcgcc gatgatgaaa
     3541 gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc ccattgcggt acgccagcag
     3601 tggcgcggtg cgatggagtg taacggcaac ggtttatgct tcaactttga tgcccgtagt
     3661 ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc attcaccgaa agggcgcgca
     3721 acgctggtgc gtgaatggct gcgcttgttg gcggatcgcg gcgttgaccc actcaaactg
     3781 gaacaagaac tgcctgaatc cggcgtcagt ttgcggacgt taattgcccg cacgcgcaat
     3841 agctggcatg cgaataaagg cgaatatgac ttctcacacg aagtcaaaga ggcgatgtcg
     3901 ggctgtctgg cctgtaaagc ttgttcgacc cagtgcccca tcaaaattga tgtgccggag
     3961 tttcgctctc gttttctgca gctctatcac acccgttatt tacgcccgct tcgcgaccac
     4021 ctcgttgcta cggtcgagag ctacgcaccg ctgatggcac gcgcgccgaa aacctttaac
     4081 ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg catcgttgat
     4141 ttgccactgc tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg ctcggcaaac
     4201 atgacgctgg aacagcttga agctctcaat gcagagcaga aagcgcgcac ggtgttggtg
     4261 gtgcaggacc cctttaccag ctattacgat gcgcaagtgg tggcggattt tgtccgtctg
     4321 gttgaaaaat taggtttcca gcctgtgtta ctgccatttt cgccaaatgg caaagcccag
     4381 catatcaaag gctttcttaa tcgttttgcg aagacggcga aaaagacggc ggatttcctc
     4441 aaccgtatgg ctaagcttgg gatgccaatg gtgggcgtcg atccggcgct ggtactttgt
     4501 tatcgcgatg aatataaact ggcgctgggc gaggaacgtg gcgagtttaa cgtcttactt
     4561 gcgaatgaat ggctggcaag cgcacttgag tcacatccag tggctacagt cagcggtgaa
     4621 tcatggtatt tctttggtca ctgtaccgag gttaccgctt tgccgggcgc gccagcacaa
     4681 tgggccgcga tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt gggttgctgc
     4741 ggcatggcag ggacttacgg acatgaagcg aaaaaccatg aaaattcgct cgggatctat
     4801 gagttatcct ggcatcaggc tatgcagcga ctgccgcgta accgctgtct ggcgaccgga
     4861 tattcctgcc gtagccaggt aaaacgggtt gaaggcacgg gggtacgcca tcctgtgcag
     4921 gctttactgg agattattaa atgatatgga aacggaaaat caccctggaa gcactgaatg
     4981 ctatgggtga aggaaacatg gtggggttcc tggatattcg ctttgaacat attggtgatg
     5041 acacccttga agcgacaatg ccagtagact cgcggacaaa gcagcctttc gggttgctgc
     5101 atggaggagc atccgtggta ctggccgaaa gtatcggttc cgttgccggt tatttatgta
     5161 ccgaaggtga gcaaaaagtg gttggtctgg aaatcaatgc taatcacgtc cgctcggcac
     5221 gagaagggcg ggtgcgcggc atatgtaaac cgttgcatct cggttcgcgt caccaggtct
     5281 ggcagattga aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga ttgacgaccg
     5341 ccattttgtg acgttgtcac cagaaaagtg tgacgcatat tgcttttggt cattatctgc
     5401 acagtaaacg gtggtatact gtgcaggtct tgtctaaaag cgaggatgct atgtctactc
     5461 aacttgatcc cacccagctg gccattgaat ttttacgccg tgaccagagc aatctttcac
     5521 ctgcccaata tctcaaacgt ctgaaacaac tagaacttga atttgctgac ctgcttactc
     5581 tctcttctgc tgaattaaaa gaagaaatct actttgcctg gcgtctgggc gtgcattgat
     5641 cctgacttaa tgccgcaagt tcgccaaatc gacctggatt aacctccgtc caggttcgat
     5701 gaccttctta atactgctta tctaccgctt gatattgttc aaaaataata ctaatcgcag
     5761 tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct tcaagagcta
     5821 agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa gcctgtgtcg
     5881 cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat ctcttaaata
     5941 gtcattttca atacaacttt ttctttttca tcacagcatt aatgctcctt agataataac
     6001 cattatctaa caatgagata cctaattctt agcgtgcctg ttaacccaca catcaggggc
     6061 tatgcttatt aaaagtaggc aaaaagcatg ttttacggta aagcccctgc gtttgctggg
     6121 ttgaactgat aatcattatc actaacatgc tgttatacgc tgaaagcgat gaagtgaggt
     6181 aaatagatgg acatgcattc aggaaccttt aacccacagg atttcgcctg gcaaggctta
     6241 acgctgacac ccgcagcggc gatacacatc cgcgagctgg tggcaaagca gccaggtatg
     6301 gtcggcgtgc gcttaggtgt gaagcaaacg ggctgcgcgg gctttggcta tgtgctcgac
     6361 agtgttagcg agccggacaa agacgatctg ctgtttgaac acgacggcgc gaagctgttt
     6421 gtcccgctgc aatcgatgcc gtttattgat ggcacggaag tcgatttcgt tcgtgaagga
     6481 cttaatcaga tattcaaatt tcacaaccct aaagcccaga atgaatgtgg ctgtggcgaa
     6541 agctttgggg tataggcggt actatgtctc gtaatactga agcaactgac gatgtcaaaa
     6601 cctggaccgg cggcccgctg aattataaag aaggattctt cacccagtta gccaccgatg
     6661 agctggcaaa ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag cgtaatgagc
     6721 cggagtggat gctggagttt cgtctaaacg cctatcgcgc atggctggag atggaagaac
     6781 cgcactggtt gaaagcgcac tacgacaagc tgaattatca ggattacagc tactactcag
     6841 caccatcgtg cggtaattgt gacgacactt gcgcgtctga acctggcgcg gtgcagcaaa
     6901 ctggcgcgaa cgccttttta agtaaagagg tggaggcggc gtttgagcag ttgggcgttc
     6961 ccgtgcggga aggcaaagag gtggcggtgg atgccatttt cgactctgtt tcggttgcca
     7021 ccacttatcg cgaaaaactg gcggagcagg gaattatttt ctgttccttt ggtgaggcga
     7081 tccacgatca cccggaactg gtgcgtaaat atctcggcac cgtggtgccg gggaatgaca
     7141 acttctttgc agcacttaat gcggcggtag cctctgacgg tacgtttatt tatgtgccta
     7201 aaagcgtgcg ctgcccgatg gaactttcca cctattttcg cattaacgcg gaaaaaaccg
     7261 ggcagtttga gcgcacaatt ctggtggccg acgaagacag ctacgtcagc tacattgaag
     7321 gctgttccgc tccggtgcgt gacagctatc agttgcacgc ggcagtggtg gaagtcatca
     7381 tccataaaaa cgccgaggtg aaatattcca cggtacaaaa ctggttccct ggcgataaca
     7441 acaccggcgg tattctcaac ttcgtcacca agcgtgcttt gtgcgaaggc gaaaacagca
     7501 aaatgtcatg gacgcaatca gaaaccgggt cagcgattac gtggaaatat cccagctgca
     7561 ttttgcgcgg cgataactcc attggtgagt tttactcagt ggcgctgacc agcggtcatc
     7621 agcaagcgga taccggcacc aagatgatcc acatcggtaa aaacaccaaa tcgaccatta
     7681 tctcgaaagg gatctctgcc ggacatagtc agaacagtta tcgcggctta gtgaaaatca
     7741 tgccgacggc aaccaatgcg cgcaatttca ctcagtgcga ctcaatgctg attggcgcta
     7801 attgtggggc gcataccttc ccgtatgtcg aatgtcgcaa taacagcgca caactggagc
     7861 acgaggcaac gacatcacgt attggtgaag atcaactgtt ttactgcctg caacgcggga
     7921 tcagcgaaga agacgccatc tcgatgattg ttaacggttt ctgcaaagac gtgttctcag
     7981 aactgccgct ggaatttgcc gttgaagcac aaaaactcct cgccatcagt cttgaacaaa
     8041 gcgtcggata aggaataaac atgttaagta ttaaagattt acacgtcagc gtggaagata
     8101 aagctatcct gcgtggatta agcctcgacg ttcgtcccgg cgaagttcac gccattatgg
     8161 ggccaaacgg ttcgggcaaa agtaccttat cggcaacgct tgccgggcgc gaagattatg
     8221 aagtgacggg cggcacggtt gagttcaaag gcaaagattt gcttgcactg tcgccggaag
     8281 atcgtgcagg cgaaggcatc tttatggcct tccagtatcc ggtggagatt ccaggcgtca
     8341 gtaaccagtt tttcctgcaa acggcactta atgcagtgcg cagctatcgc gggcaggaaa
     8401 cgctcgaccg ctttgatttt caggatttga tggaagagaa aatcgctctc ctgaagatgc
     8461 cggaagattt attaacccgt tcggtaaacg ttggtttttc cggcggcgag aaaaagcgca
     8521 acgatatttt gcaaatggcg gtgctggaac cggagttatg cattcttgat gagtcggact
     8581 ccgggctgga tattgacgca ttaaaagtgg tcgccgatgg cgtgaactcg ctgcgtgatg
     8641 gcaagcgctc attcatcatt gttacgcact accaacgcat tctcgactac atcaagcctg
     8701 attacgttca tgtgctatat cagggacgaa ttgtgaaatc cggcgatttc acgttggtca
     8761 aacaactgga ggagcagggt tatggctggc ttaccgaaca gcagtaatgc gctgcaacag
     8821 tggcatcact tgtttgaagc tgaagggaca aaacgctcac cgcaagcaca gcagcattta
     8881 caacaattgc tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa atatacgccg
     8941 ctggaagggc tgaccaatag tcagtttgtc agcattgcgg gagatatatc cccacagcag
     9001 cgtgatgcct tagcgttaac gttagacgcc gtgcggctgg tgtttgtcga tggacgttac
     9061 gtgcccgcgc tgagcgatgc aattgaaggc agcggatatg aagtgagcat taacgacgac
     9121 cgtcaggggt tacccgacgc tattcaggcg gaagtgtttc tgcatttgac ggaaagcctg
     9181 gcacaaagcg tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa gccattgctg
     9241 ttaatgcata tcacccaggg cgtggcaggt gaagaggtga acactgccca ttaccggcat
     9301 catctggatc tggcggaagg tgccgaagca acggtgatcg aacattttgt cagccttaat
     9361 gatgctcgtc attttaccgg ggcacggttc actatcaatg tcgcagcgaa cgcccacttg
     9421 cagcatatca agctggcgtt tgaaaacccg gtcagtcacc actttgccca taacgatttg
     9481 ttgctggctg acgatgccac cgcatttagc catagtttcc tgctgggtgg cgcagtgtta
     9541 cgacacaaca ccagtacgca actcaatggc gaaaacagca cgctgcggat caatagcctg
     9601 gcgatgccgg tgaaaaacga ggtgtgtgat acccgtacct ggctggaaca caataaaggt
     9661 ttttgtaaca gccgacagtt gcacaaaact atcgtcagcg acaaaggccg cgcggtattt
     9721 aacggtttga tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat gaccaacaat
     9781 aatctgctga tgggtaaact ggcggaagtg gatacaaaac cgcagctgga aatctatgca
     9841 gatgatgtga aatgcagcca cggcgcgacg gtggggcgta ttgatgatga acagatgttc
     9901 tatctgcgct cgcgcgggat caatcagcag gatgcccagc agatgatcat ttacgccttt
     9961 gctgccgaac tgacggaatc actgcgtgat gaggggctta aacagcaggt gctggcccga
    10021 atcggtcaac ggctgccagg aggtgcaaga tgactttttc cgtcgacaaa gtgcgggccg
    10081 actttccggt gctttcgcgt gaggtaaacg gtttgccgct ggcttatctc gacagcgccg
    10141 ccagtgcaca gaaaccgagc caggtgattg acgccgaggc cgagttttat cgtcatggct
    10201 acgcggcggt gcatcgcggt attcatacct taagcgccca ggcgaccgag aaaatggaga
    10261 acgtgcgcaa gcgggcatcg ctgtttatta atgcccgttc ggcggaagag ctggtgttcg
    10321 tccgcggcac gacggaaggg atcaatctgg tcgccaatag ctggggcaac agcaatgtgc
    10381 gggcgggcga taacatcatc atcagtcaga tggagcacca cgctaacatt gtcccctggc
    10441 agatgctttg cgcacgcgtt ggcgcagagc tgcgtgtgat ccctctcaac cccgacggta
    10501 cgctgcaact ggagacgctg cctacgctgt ttgatgagaa aactcgcctg ctggcaatta
    10561 ctcatgtctc caacgtgctt ggcacagaaa atccactggc ggaaatgatc acgcttgcgc
    10621 accagcatgg cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat catccggtgg
    10681 atgttcaggc gctggattgc gacttttacg tgttctccgg gcataaactg tatggcccca
    10741 ccggaattgg cattctttat gtgaaagaag ccttgttgca ggagatgccg ccgtgggaag
    10801 ggggcggttc tatgatcgcc accgtcagcc tgagtgaagg cactacctgg accaaagcac
    10861 catggcggtt tgaagccggt acacccaata ccgggggcat cattggtctt ggcgcggcgc
    10921 tggagtatgt ttcggcgctg gggcttaata gcatagccga gtatgaacag aatctgatgc
    10981 attatgcgct atcacagctg gaatctgtac cggatctcac tctctatggc ccacaaaaca
    11041 ggcttggcgt tattgctttt aatctcggta aacaccacgc ctatgatgtt ggcagttttc
    11101 tcgataatta cggcattgct gtgcgtaccg gacatcactg cgctatgcca ttaatggcct
    11161 attacaacgt ccctgcgatg tgtcgggcgt cgctggccat gtataacacc catgaagaag
    11221 tggatcgtct ggtgaccggc ctgcaacgta ttcaccgtct gctgggataa cagggaggca
    11281 ctatggcttt attgccggat aaagaaaaat tgctgcgtaa ttttttacgc tgcgccaact
    11341 gggaagagaa atatctctac attattgagc tgggccagcg tctgccagaa ttacgcgccg
    11401 aagaccgaag cccacaaaat agcattcagg gctgccagag tcaggtgtgg attgtcatgc
    11461 gccagaatgc gcagggaatt attgaattgc agggcgacag cgatgcggcg attgtgaaag
    11521 ggcttattgc ggtcgtcttt attctctacg atcagatgac gccgcaggat attgtcaatt
    11581 tcgatgtgcg tccgtggttt gaaaaaatgg cgctcaccca acatctcacc ccatctcgtt
    11641 cacaaggtct ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca cttagctaaa
    11701 ctaaagggac agctttcatc cggttggctc acattgccgg atggtcaatg tttaaactga
    11761 cctgcacagg agtgttttgg tttcaggtga gcataagcgc catccgacgt ttcagcgtga
    11821 gtctccggca aatacaggag gtttacaatg aaacgcgcgt ctttgcttac actcacgctt
    11881 atcggcgctt ttagcgccat ccaggctgcc tgggcggttg attatccgct accaccaacc
    11941 ggaagccgac tggttgggca aaatcaaacg tatacggtgc aagaagggga taaaaacctt
    12001 caggctattg cccgacgttt tgatactgcg gcaatgttga tccttgaagc caataacact
    12061 atcgccccgg tgccaaaacc tggtacgacg ataactattc cttcacaact gttattacct
    12121 gatgcaccgc gtcaggggat tatcgttaac cttgcagagc tgcgccttta ttattatccg
    12181 ccgggagaaa atattgtgca ggtttatcca ataggtattg gattgcaggg gctggaaacg
    12241 ccggtgatgg aaacgcgtgt tgggcagaaa atccctaacc caacctggac gcctacggca
    12301 ggcattcgtc agcgttcgct ggagcgtggc attaaattac cgccagtcgt tcctgccgga
    12361 ccaaataacc cgctaggacg ttacgcactg cgcctcgcgc atggtaatgg cgaatacctc
    12421 attcatggca ccagtgcgcc ggacagcgtt ggtttgcgcg tcagttcagg gtgtattcgc
    12481 atgaatgcac cggatattaa agccttgttc tccagcgtgc ggacgggaac gccggtgaaa
    12541 gtgatcaacg aaccggtgaa atattccgtg gaacctaacg ggatgcgtta tgttgaagta
    12601 catcgaccac tatcagcaga agaacagcaa aacgttcaga caatgccata cacactgcca
    12661 gcaggcttta cgcaatttaa agacaataag gctgtagatc agaagttagt cgataaagcg
    12721 ttgtatcgtc gggcagggta tccggtttcg gtgagcagtg gagcaactcc cgcagccagc
    12781 aatgcgcctt cagtagagtc agcgcagaat ggtgaaccag agcaagggaa tatgttacgc
    12841 gcgacgcagt agtggtaaat agcagacaaa aaaaatggcg cacaatgtgc gccatttttc
    12901 acttcacagg tactattact tgcggtattt agtagccatg ttgtccagac gctggttagc
    12961 acgagctgcg tcatctttag cagcctgaac gtcggaacgc attgcgttca cgtcgttgct
    13021 cagctggtca actttagcgt tcagagtctg aacgtcagaa gacagctgat cgattttagc
    13081 gttgctggag caacctgcca gcagagtaga acccaggatt accgcgccca gtaccagttt
    13141 agtagctttc attattaata ccctctagat tgagttaatc tccatgtagc gttacaagta
    13201 ttacacaaag ttttttatgt tgagaatatt tttttgatgg gaaggcactt atttttgaac
    13261 gttcgctcaa agaagcatcg aacgctgggt tttagataaa aaaattgaga gaaaacagga
    13321 tgcttccatc ggattcatct tagataaagt gagattatat agtgaaatcc gatttgattt
    13381 tttattgctt ctggttatcg attaaataaa aaaagcgccc atcagggcgc ttcgatatac
    13441 aaattaattc acaaaagcaa tattacagga cgtgaacaga tgcggtgtta gtagtgccgc
    13501 tcggtaccag tgcaccagaa accataacta cgacgtcacc tttgtgtgcc agaccgctct
    13561 gcagagccag ttctttaccc agacggtaga aatcatcagt agaagtgatc tctttaacca
    13621 gctgcggcac aacgcctttg ctcagtacca actgatgagc cgttttttcg ttggtggtca
    13681 gtgccaggat ggtggcattc gggaagtatt tacgtactgc gcgagcagat ttaccgccct
    13741 gggtagcaac cacgatcagc ggagcatcca gtttttcagc agtttcaacg gcaccacggc
    13801 ataccgcttc ggtgatgcgc agtttacggt tgtcattgtt gaactcgaga cggctgttca
    13861 tcacgcggtc ggtacgttcg cagatggtcg ccatgataga aaccgcttcc agcgggtatt
    13921 taccttttgc ggattcacca gacagcatca ctgcgtcagt accgtcgagg atggcgtttg
    13981 caacgtcacc ggcttctgcg cgagtcgggc gtgggttttt gatcatggaa tccagcatct
    14041 gggtcgcagt gataacgact ttacgtgcac ggatacattt ttcgatcatc atcttctggg
    14101 cgaagataac ttcttctacc gggatttcta cacctaggtc gccacgcgca accatgatgc
    14161 cgtcagaggc ttcgaggatt tcgtcgaagt tgttgaggcc ttcctggttt tcgattttgg
    14221 agatgatgtg gatgttttcg ccgccgtgcg ctttcaggtg ctcacggatt tcgataacgt
    14281 cagaacgctt acgaataaag gaagcagcaa caaagtctac gcctttttcg caaccaaaga
    14341 tcaggtcctg tttgtctttt tcagccagtg ctggcagagc aatggaaacg ccaggcaggt
    14401 tcacaccttt gttttcgccc aggtcaccgt tgttcagcac tttacagata actttgttac
    14461 cttcaatggc ggtaacttcc ataccgatca gaccatcgtc aaccagtacg gtgttgccaa
    14521 cagacaggtc agtagtgaaa ccttcatacg ttaccgcaac catttcgctg ttgccgataa
    14581 cagatttatc agtggtgaaa gtaaaggtct gaccagcttt cagagaaaca tcgttaccgc
    14641 cttccagttt catggtgcgg atttccggac ctttggtatc aagcaggata gcggcggttt
    14701 taccagtttt gctcatcacg ttgcgcagat tctgaatgcg ctgaccgtgt tctgcatagt
    14761 caccatgaga gaagttcaga cgcataacgt tcatgccagc gtccagcatt ttagctaaca
    14821 tctcttcaga ttcggttttc ggtccgatgg tgcaaacaat tttggtcttt ttcatgacag
    14881 tcttagtctt taagttgaga aggatgggag aaacttgctt tctgggcgca ctgccggaaa
    14941 gcgaatccgg tattacgaaa gtggtggtgc ctcgctctaa ggataggtga catcgaaaaa
    15001 gcgtgcagag gaatgtgtgc ttgtgtctca gcccaacgtg agtgcctgtt tttgagtcgt
    15061 tgagttctac agtccaaagt gctgaaacca ttcaagagtc aattggcgcg cattatacgc
    15121 tgaaattaat ccaaaaggaa aacaaaaaca gcgtttcaaa aaatatacaa cttgttagac
    15181 aataaattgt tatcaaagat ttaacgttcc agggaaaagg ttacgcaaaa acttcgctgt
    15241 ttgcgcaacg tctggtgccg aagttgtcag tgtgaggata agtgaaagtt catccggtgt
    15301 ataaatatat catcgctcaa ttgctacagt taaaataatt tatcgcgtta tttgtctgat
    15361 tttgtttgat ttttgtttaa ttggttagat tgatatatgc tgatagttgg catgataaat
    15421 aggtataaag aatgggaaat cggacaaaag aggatgaact gtaccgagaa atgtgcagag
    15481 ttgtcggtaa agttgtgctg gaaatgcgcg atctggggca ggaaccaaag catattgtta
    15541 tcgcaggcgt attacgtaca gcattagcca ataaacgcat tcagcgcagt gagctggaaa
    15601 agcaggcgat ggaaaccgtc attaacgcac tggtaaaatg accattc
//
LOCUS       DADLSA010000092        15227 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.092, whole genome shotgun sequence.
ACCESSION   DADLSA010000092 DADLSA010000000
VERSION     DADLSA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.092"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            214..1515
                     /gene="rstB"
                     /locus_tag="JN691_002791"
     CDS             214..1515
                     /gene="rstB"
                     /locus_tag="JN691_002791"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAY7244198.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            1591..2520
                     /gene="tus"
                     /locus_tag="JN691_002792"
     CDS             1591..2520
                     /gene="tus"
                     /locus_tag="JN691_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAY7244199.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2517..3920)
                     /gene="fumC"
                     /locus_tag="JN691_002793"
     CDS             complement(2517..3920)
                     /gene="fumC"
                     /locus_tag="JN691_002793"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAY7244200.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(4063..5709)
                     /locus_tag="JN691_002794"
     CDS             complement(4063..5709)
                     /locus_tag="JN691_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAY7244201.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5908..7083
                     /gene="manA"
                     /locus_tag="JN691_002795"
     CDS             5908..7083
                     /gene="manA"
                     /locus_tag="JN691_002795"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAY7244202.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            7184..8692
                     /locus_tag="JN691_002796"
     CDS             7184..8692
                     /locus_tag="JN691_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY7244203.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8918..10183)
                     /gene="uidC"
                     /locus_tag="JN691_002797"
                     /pseudo
     CDS             complement(8918..10183)
                     /gene="uidC"
                     /locus_tag="JN691_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(10222..10389)
                     /locus_tag="JN691_002798"
     CDS             complement(10222..10389)
                     /locus_tag="JN691_002798"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HAY7244204.1"
                     /translation="MGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKK
                     VQQQLISDITN"
     gene            complement(10405..11401)
                     /gene="uidB"
                     /locus_tag="JN691_002799"
                     /pseudo
     CDS             complement(10405..11401)
                     /gene="uidB"
                     /locus_tag="JN691_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            complement(11398..13209)
                     /gene="uidA"
                     /locus_tag="JN691_002800"
     CDS             complement(11398..13209)
                     /gene="uidA"
                     /locus_tag="JN691_002800"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAY7244205.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLDIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(13597..14187)
                     /locus_tag="JN691_002801"
     CDS             complement(13597..14187)
                     /locus_tag="JN691_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7244206.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(14416..15183)
                     /gene="hdhA"
                     /locus_tag="JN691_002802"
     CDS             complement(14416..15183)
                     /gene="hdhA"
                     /locus_tag="JN691_002802"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HAY7244207.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFAWRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         3841 a         3939 c         3603 g         3844 t
ORIGIN      
        1 agactaaaga acaacaaagc gtcacgttaa attaaacctt agcataataa acaagccccg
       61 gtggaaaatt gaatttactg aatatgaata cgccggggat acatccgtct gttccggaca
      121 ttatcttcca aacaaggtct tatccagttg gcggtttaca ttgctccaca gtcgtgaatt
      181 aatacgaata gtattctgtt gcgggcgatg ccaatgaaaa aattgtttat ccagttttac
      241 ctgttattgt ttgtctgctt ccttgtgatg tctctgctgg ttgggctggt gtacaaattt
      301 accgccgaac gcgcgggcaa acagtcgctg gatgatttga tgaacagttc gctgtatctg
      361 atgcgcagcg aattgcgaga gatcccccca cacgactggg gtaagacgct gaaagagatg
      421 gatttaaatc tctctttcga tctgcgtgtc gagccactga gtaaatacca tcttgatgat
      481 atttccatgc accgactgcg tggcggcgaa attgtcgccc tggacgatca gtacacgttt
      541 ttgcagcgta tcccgcgcag ccactacgtg ctggcagttg gtcctgttcc ttatctttat
      601 tacctccatc aaatgcgatt gctggatatc gccctgatcg cttttattgc tatttccctc
      661 gcctttccgg tgtttatctg gatgcgtccg cactggcagg atatgttaaa actggaagca
      721 gcggcgcaac gatttggcga tgggcatctc aatgaacgta tccactttga tgagggttcg
      781 agctttgaac gacttggtat cgcatttaac cagatggcgg acaatatcaa cgccttaatt
      841 gccagcaaaa aacagcttat tgacggtatc gctcacgaac tgcgaacacc gttagtgcgc
      901 ctgcgttatc gactggagat gagcgataac ctgagcgccg ccgaatccca ggcgttgaat
      961 cgtgatatca gtcaacttga agctttaatt gaagagctgc tgacttatgc ccgactcgat
     1021 cgcccacaaa acgagcttca tcttagcgaa ccagacctgc cgttgtggct gtcaacgcat
     1081 ctggcagata ttcaggcagt aacgcccgat aaaacggtac ggataaaaac gctcatgcaa
     1141 ggccattatg cggcgttgga tatgcgctta atggagcgcg tgctggataa tttgctcaat
     1201 aacgccctgc gctactgcca ttcaacggtt gaaaccagcc tgctactgtc ggggaataga
     1261 gcgacattaa ttgttgagga tgatggccca gggattgccc cagaaaaccg cgaacatatc
     1321 tttgaacctt ttgttcgcct cgatcccagc cgggatcgct caaccggcgg ctgcgggctg
     1381 gggctggcaa ttgtccactc tatagcactg gcaatgggcg gtacggttaa ttgtgacacc
     1441 agcgaactgg gtggtgctcg cttctcgttt agctggccgt tatggcataa catcccgcaa
     1501 tttacctctg cctgacacta cgcgcacgat ggtcaagtca ccacgactgt gctataaaat
     1561 aagtatgttg taactaaagt ggttaatatt atggcgcgtt acgatctcgt agaccgactc
     1621 aacactacct ttcgccagat ggaacaagag ctggctacat ttgccgctca tcttgagcaa
     1681 cacaagctat tggttgcccg cgtgttctct ttgccggagg taaaaaaaga ggatgagcat
     1741 aatccgctta atcgtattga ggtaaaacaa catctcggca acgacgcgca gtcgctggcg
     1801 ttgcgtcatt tccgccattt atttattcaa caacagtccg aaaatcgcag cagcaaggcc
     1861 gctgtccgtc tgcctggcgt gttgtgttac caggtcgata acctttcgca agcagcgttg
     1921 gtcagtcata ttcagcacat caataaactc aagaccacgt tcgagcatat cgtcacggtt
     1981 gaatcagaac tccccaccgc ggcacgtttt gaatgggtgc atcgtcattt gccggggctg
     2041 atcaccctta atgcttaccg cacgctcacc gttctgcacg accccgccac tttacgcttt
     2101 ggttgggcta ataaacatat cattaagaat ttgcatcgtg atgaagtcct ggcacagctg
     2161 gaaaaaagcc tgaaatcacc acgcagtgtc gcaccgtgga cgcgcgagga gtggcaaaga
     2221 aaactggagc gagagtatca ggatatcgct gccctgccac agaacgcgaa gttaaaaatc
     2281 aaacgtccag tgaaggtgca gccgattgcc cgcgtctggt acaaaggaga tcaaaaacaa
     2341 gtccaacacg cctgccctac accactgatt gcactgatta atcgggataa tggcgcgggc
     2401 gtgccggacg ttggtgagtt gttaaattac gatgccgaca atgtgcagca ccgttataaa
     2461 cctcaggcgc agccgcttcg tttgatcatt ccacggctgc acctgtatgt tgcagattaa
     2521 cgcccggctt tcatactgcc gaccatctgt tctggccgta cccagctgtc aaactcggct
     2581 tcgctaagat accccagcgc aagggccgca gcttttaagg tcagcccttc tttatgcgct
     2641 tttttggcga tctccgcggc tttgtcataa ccaatgtggg tgttaagcgc agtcaccagc
     2701 atcagcgatt cattgagtaa ttgattgatt cgctcacgat tcggttcaat acccactgcg
     2761 cagtgtttgt taaaactttc catgccatct gccagcaagc gcaccgattg caggaaatta
     2821 tggatcacca tcggacggaa gacgttcagt tcaaagttac cggaagcgcc acccatgttg
     2881 atcgccacgt cgttccccat tacctgacag cagagcatgg ttaatgcttc acactgggtt
     2941 gggttcactt tccccggcat gattgagctg cccggctcat tttccgggat tgagatttca
     3001 ccaattccgc agcgcgggcc agaggccagc cagcggacat cattggcgat tttcatcagt
     3061 gacgcagcca accctttcaa cgcgccgtgc gcctgaacca gggcatcaca ggtcgcaagc
     3121 gcttcaaatt tgttcggcgc ggtaacaaac ggtgcacagg taatgactgc cagttcatct
     3181 gctacgcgac gcgcatactc cggatgggta tttagtccag tacccaccgc tgtaccgcca
     3241 agagccagtt ccgctacgtg aggcaggctg tattcgatat gtttgagatt atgctcgagc
     3301 atcgctaccc agccggaaat ctcctgccct agtgttagcg gcgtggcgtc ctgcaagtgg
     3361 gttcgaccga ttttgacgat atcggcaaat gcacgcgatt tttcactcag cgtctgtgtc
     3421 agggttttaa gctgcggaat gagttgcttg cgcagtgcca gcagcgccgc aacgtgcatc
     3481 gccgtcggaa agacatcgtt ggaactttgg cttttgttca cgtcgtcgtt agggtgaact
     3541 ttacgttcca tcccgcgcac accgccgagt aattcactgg cccggttagc cagcacttcg
     3601 ttcatgttca tgttactttg cgtgccggag ccggtctgcc agatagccag cgggaattcg
     3661 tcgtcatgct gtcctgccag tacttcatcc gccgcctgcc gaatggcgct cgctttctct
     3721 tcagacaaca agtctaaatc ttcattaact tttgccgctg cgcgcttggt tagcgccagc
     3781 gcatgaatca gtgatgtggg cattttctcc gtcgaaatgc ggaaatgctc cagcgagcgt
     3841 tgagtttgtg cgccccacag cttatctgcc gggacatcaa tcgcccccat cgaatctttt
     3901 tcgctgcgta ctgtattcat ggcctgctcc tcacctgatt aattttttct ttctgttttg
     3961 ctttcgttaa gcaactttta gctcactcat tatttactat ttgataacaa atgtttggtc
     4021 tttcgtgcca tgtaaaaaaa ccgccccgaa gggcggctct gtttatttca cacagcgagt
     4081 gcactgtgtg agttgtatct gctggaagaa gtcatttcct ttatcatcca caaggataaa
     4141 cgccgggaaa tcttccactt caattttcca gatggcttcc attcccagtt ccggatattc
     4201 aacacattcc aggctcttaa tacttccctg cgccaataca gcggccggac caccgatact
     4261 gccaaggtag aagccgccgt gttttttaca ggcatccgtc acctgctggc tgcggttgcc
     4321 tttcgccagc atgatcatac ttccgccctg cgcttgcagt tgatcgacat aagaatccat
     4381 ccgtccggcg gtcgttgggc caagagaacc ggaggcataa ccttccggcg ttttggccgg
     4441 acccgcgtag taaatcggat gatctttaat gtattgcggc agcccttcac cgttatccat
     4501 ccgctctttc agtttggcgt gagcaatatc acgaccaacg ataatcgtgc cgttaagcga
     4561 taagcgtgta gaaacgggat actgcgacaa ctgtgcgagg atctctttca tcggacggtt
     4621 aaggtcaacg cgcaccgctt cgccttctcc cgctttacgc agctcttcag ggatatattt
     4681 acctggatta tgttccagtt tttcgatcca gatcccctga cggttgatct tcgctttgat
     4741 attacggtca gcagagcagg aaacacccat accaaccgga caggatgcgc cgtgacgtgg
     4801 caggcgaatc acgcggatgt cgtgagcgaa gtatttacca ccaaactgcg cacccagacc
     4861 gagattttgc gcttcgatca gtaattcttt ttccagttcc acatcgcgga acgcctgacc
     4921 gtgctcattc ccttccgttg gcagttcatc atagtatttc gcggaagcca gtttcaccgt
     4981 tttaaggttc gtttctgcag aagttccacc aataacgaac gcaatatgat acggaggaca
     5041 ggccgcggta cccagcgtgc gcatcttctc aaccaggtaa tttttcagtt tccccggcgt
     5101 cagtaacgct ttggtttcct gatagagata cgtcttgttt gccgaaccac caccttttgc
     5161 gatacagagg aatttgtatt cgtcgccatc aacggcataa agatcgatct gcgctggcag
     5221 attggtgccg gtattcactt ctttatacat atccagcggc gcgttttgcg agtagcgcag
     5281 attatcttcg atataagtgt tatagacacc gcgcgccagc gccgcttcat caccaccacc
     5341 ggtccataca cgctgccctt ttttaccaac aataatcgcg gtgccggtat cctgacaggt
     5401 tggcagaacg cctttcgccg cgatgtcgga gttacgcagg aattgcagcg ccacatattt
     5461 atcattttcg ctggcctccg ggtcacgcag aatgtcggcc acctgttgtt ggtgagccgg
     5521 acgcagcata aacgacgcat catgaaacgc ctgacgcgcc aacagagtta acgcttcggg
     5581 tgcgactttc aaaatctcct gcccttcaaa ttcagatacg ctaacgtgtt cactggttag
     5641 caggtaatac tcagtatcat cttttttgag tggaaaagga gactgataat gaaagggttt
     5701 gtttgacatt gttctctcac ttactgcctg gtttggttat gctctgggcg ggtgttccgt
     5761 tgccctgtta aaagcgagta acaatatcct acacagtttt ttaacaaaaa ctgagactag
     5821 tacgactttt tgcggctcca ggttacttcc cgtaggattc ttgctttaat agcgggatta
     5881 atttccacat taaaacaggg attgatcatg caaaaactca ttaactcagt gcaaaactat
     5941 gcctggggta gcaaaacggc gttgactgaa ctttatggta tggaaaatcc gtccagccag
     6001 ccgatggccg agctgtggat gggcgcacat ccgaaaagca gttcacgagt gcagaatgcc
     6061 gccggagata tcgtttcact gcgtgatgtg attgagagtg ataaatcgac tctgctcgga
     6121 gaggccgttg ccaaacgctt tggcgaactg cctttcctgt tcaaagtatt atgcgcagca
     6181 cagccactct ccattcaggt tcatccaaac aaacacaatt ctgaaatcgg ttttgccaaa
     6241 gaaaatgccg caggtatccc gatggatgcc gccgagcgta actataaaga tcctaaccac
     6301 aagccggagc tggtttttgc gctgacgcct ttccttgcga tgaacgcgtt tcgtgaattt
     6361 tccgagattg tctccctact ccagccggtc gccggtgcac atccggcgat tgctcacttt
     6421 ttacaacagc cacatgccga acgtttaagc gaactgttcg ccaacctgtt gaatatgcag
     6481 ggtgaagaaa aatcccgcgc gctggcgatt ttaaaatcgg cgctcgacag tcagcagggt
     6541 gaaccgtggc aaacgattcg tttaatttct gaattttacc cggaagacag cggcctgttc
     6601 tccccgctgt tactgaatgt ggtgaaattg aatcctggcg aagcgatgtt cctgttcgct
     6661 gaaacaccgc acgcttacct gcaaggcgtg gcgctggaag tgatggcgaa ctccgataac
     6721 gtgctgcgtg caggtctgac gcctaaatac attgatattc cggaactggt tgccaatgtg
     6781 aaattcgaag ccaaaccggc taaccagttg ttgacccagc cggtgaaaca aggtgcagaa
     6841 ctggacttcc cgattcctgt ggatgatttt gccttctcgc tgcatgacct tagtgataaa
     6901 gaaaccacca tcagccagca gagtgccgcc attttgttct gcgtcgaagg cgatgcaacg
     6961 ttgtggaaag gttctcagca gttacagctt aaaccgggtg aatcagcgtt tattgccgcc
     7021 aacgaatcac cggtgactgt caaaggccac ggccgtttag cgcgtgttta caacaagctg
     7081 taagagctta ctgaaaaaat taacatctct tgctaagctt attaaaggct tataacacct
     7141 tcaggcggcc agtccgcctg atttcatttt atggataatc attatgaata aatcgctggt
     7201 agcggtaggc gtcattgttg cgctaggcgt agtctggaca ggcggcgcat ggtatacagg
     7261 caagaagatt gaaacccatc tcgaagacat ggtcgcgcag gcgaacgcgc aactcaaact
     7321 gacagctcct gaatccaacc tggaagtgag ttatcaaaac tatcatcgcg gcgtattcag
     7381 cagccagttg caactgttgg tgaaacccat tgccgggaaa gaaaatccgt ggattaaaag
     7441 cggtcagagc gtcatcttca acgaatcggt tgatcatggt cccttcccgc ttgcccagct
     7501 taaaaaactg aacctgatcc cgtcgatggc atcaattcaa accacgctgg ttaataacga
     7561 agtaagcaaa ccactgtttg atatggcaaa aggtgaaacg ccttttgaga ttaactcgcg
     7621 cattggttac agcggtgatt ccagttccga tatttcgctc aatccactga attacgagca
     7681 aaaggatgaa aaagtcgcct ttagcggcgg cgagttccag ttaaatgctg acagagacgg
     7741 caaagccatc tccctttccg gggaggcgca aagtggtcgg atagacgcag ttaacgaata
     7801 caaccagaaa gtgcagttga cctttaataa tctgaaaacc gacggttcca gcacgctggc
     7861 aagttttggt gagcgcgtag gaaaccaaaa actgtcactg gaaaaaatga ccatttcagt
     7921 ggaaggcaaa gaactggcac tgctggaagg catggagatc agcggtaaat cggatctggt
     7981 caatgacggt aaaacgatca atagccaact ggattactcg ctaaacagcc tgaaggtaca
     8041 gaatcaggat ctgggcagcg gcaagctgac tttaaaagtc ggccagattg atggtgaagc
     8101 ctggcatcag tttagccagc aatataacgc gcaaactcag gcgctgctgg cacagccaga
     8161 aattgccaac aatcccgaac tttatcagga gaaagtgacg gaagccttct ttagcgccct
     8221 gccgctgatg ttgaaaggcg atccggtgat tactatcgcg ccgctaagct ggaaaaacag
     8281 tcagggtgaa agtgcgctga atctgtcgct gttcctgaaa gatccggcaa cgactaaaga
     8341 agcgccgcaa acgctggcgc aggaagtaga tcgttcggtt aaatctctgg atgcgaaact
     8401 gaccattccg gtggatatgg caactgagtt gatgactcag gtagcgaagc tggaaggtta
     8461 tcaggaagat caagcgaaaa aactggcgaa acagcaagtt gaaggtgcat cagcaatggg
     8521 gcagatgttc cgtctgacca ccttgcagga caataccatc accaccagcc tgcaatatgc
     8581 taacggtcag ataacgttaa atgggcagaa aatgtcgtta gaagattttg ttggcatgtt
     8641 tgcgatgcca gctcttaacg ttccggctgt accggcaatt ccgcagcagt aattcacggc
     8701 caaaacgccg gagaattttc actccggcgt tttcatctga aaacaaacca gagattgcct
     8761 gatgcgctac gcttatcagg cctacctttt cctgcaacac tttgaattta tgagtttttg
     8821 taggctggat aaagcgttta cgccgcatcc ggcataaaaa acgcgcactt tgtcaacaat
     8881 ctgaaacgcc ggaggttttt ctctccggcg ttatttctta cttcagcata aagtcatact
     8941 gaatactcat tttcacatcc cagtaattca ggcgatcgcc cagcgtactg ccatcgcgct
     9001 tttcccgccc tttatcggca gcaagaaaga aattcatccc ttgtaacggc ccttcgctga
     9061 cccgatagtt cataccacct aagatacccg cgccttcaat ccgcacatct cgtttactgg
     9121 attcatagcc gcgagtcaca atcggtgcaa atgtcagcgt aaattgcgga gttacacggt
     9181 agttgacacc taattgccag gactgcattc cttcacggtt acgatcaaga cttaatgaaa
     9241 agggatagcc aatgtcggta tccaccacgg caccattcat tgcatggcgc aaataaccac
     9301 cactgccaaa tacgcttccc cacggcgcat tctaggtcaa ttgtccacta accaacgccg
     9361 tttcattggg ctggctggta ttacgactta aaccttcgag ctgcgcgtaa aagccgagca
     9421 actccccttt caacgtataa ttcgggtcga gcggtatgct tttctcgaga atactttgta
     9481 tctgattgga aacatctttc tgtaccgatg cccccagaga atagcgactg gtatcatcat
     9541 gccaactggc ggctaaaaaa gaacgtgcgc gatgtttatc gtattttccc gtctggctgt
     9601 tatacacccc ttcatcacga ataccggttg ttgaatcagt ccataaaaat actcgtgaac
     9661 gccacattcc ccacagttca atatttggcg ttaaagccca ataacctaat gctccttcag
     9721 aaactgtcgg taatgtacgt tgcgaactgc cagccattaa cggaatacga taaggtaagc
     9781 taccataact ataatccgtc ccgaagcgac ctaatttaag caggctattt tcactgggtt
     9841 ttatttttac tgcccccagg gcaaagccaa aacttttatc accatcaaga taccaccggg
     9901 tactcatatc agcacgagca cctaatttat aaacataata cgctccgcct tcaacgccaa
     9961 taatattgga ataataacca ctattgaaat cgagcaatcc tccctgtacc catgcgtaaa
    10021 tatctcttcc cgcgccagca ctgggcttat cagccctgcg caattcgttt ttcagtctga
    10081 tgcgtagtcc cgcttcatcg tcagccagtt gcgccgcgta agcagaggta aggccagagg
    10141 cagccgtcag gcaaataacg gccatggcca ctatttttct catagttgca cctttgatgt
    10201 tctgattatt tttattgaat attaattagt gatatcgctg attaattgct gctgcacttt
    10261 tttacgatta tcaatttcaa ccacgatttc tttgaatttt ttatccgtga gcggataaaa
    10321 ccagataata acgaatgcca gtagcataaa tccgcaaggt actaaggcaa ttgatgtgcg
    10381 gatgcccata ataacttccg gcgtttacac ctgattggcg atatatccgc ttaatcccaa
    10441 aataaaggca ggaattgaac ctccgattgc ctgaccgcat ttacgggtaa atgagaatag
    10501 tgaataggtg agcccttcaa ttcgcacgcc ggtcaggtat tcaccgtatt ctacggtatc
    10561 agcttccagt gcccacatca cagtcatagt aacgccctga ccaagtgaac agcccggtat
    10621 catttaacac atagcgcacg tagaacaacg acgaggcgct gaccgcgaag gtcgaaatca
    10681 gcacacacag cgcaccgatg cacaacataa acagcgggcg attccgtttc agggtttgca
    10741 aactgatctt caatgacggc tgcgccacga tacgtaccac attttcacgc gtcgatttga
    10801 agcagatgaa gtaaagcacc attccggcaa tcgccagcac aatcgtccag aaatggtata
    10861 ccgacaccat ctcttccggg ctggagttct taatgctcgg tcctatcaga aatgccaggc
    10921 atacaaaggt caatgaagcg gcaatcccac gagccgcgcc cagacgggcg cgggattgtg
    10981 gttgttgggt catcgcggta gcaagtgaac cataaggaat attcaccagg ctgtagcaaa
    11041 gcccgaggcc catgtaggtc aaatatgcat acaccacttt gctaccatgg ctccagtcgg
    11101 tcggtaccca gaataccagc acgctgaaga tcattaacgg cgcagtaccg aagagtaaaa
    11161 acgggcggaa ttttccccag cgggtattca cactgtccac cactcgtccg gcaaagacgt
    11221 cggcgaaggc atcgaatacc cgcaccagta acagcatggt gcctgccgca gcggcaccga
    11281 cgccagcgac gtcggtgtag taactcaata ggaagagcgc ccccattgcg aaggcgaagt
    11341 tattggcgac gtcaccgagg ctgtagccga cgatggtgcg ccaggagagt tgttgattca
    11401 ttgtttgcct ccctgctgcg gtttttcacc gaagttcatg ccagtccagc gtttttgcag
    11461 cagaaaagcc gccgacttcg gtttgcggtc gcgagtgaag atccctttct tgttaccgcc
    11521 aacgcgcaat atgccttgcg aggtcgcaaa atcggcgaaa ttccatacct gttcaccgac
    11581 gacggcgctg acgcgatcaa agacgcggtg atacatatcc agccatgcac actgatactc
    11641 ttcactccac atgtcggtgt acattgagtg caggccggct aacgtatcca cgccgtattc
    11701 ggtgatgata atcggctgat gcagtttctc ctgccaggcc agcagttctt tttccagtac
    11761 cttctctgcc gtttccaaat cgccgctttg gacataccaa ccgtaataac ggttcaggca
    11821 cagcacatca aagagatcgc tgatggtatc ggtgtgagcg tcgcagaaca ttacattgac
    11881 gcaggtgatc ggacgcgtcg ggtcgagttt acgcgttgct tccgccagtg gcgcgaaata
    11941 ttcccgtgca ccttgcggac gggtatccgg ttcgttggca atactccaca tcaccacgct
    12001 tgggtggttt ttgtcacgcg ctatcagctc tttaatcgcc tgtaagtgcg cctgctgagt
    12061 ttccccgttg acagcctctt cgctgtacag ttctttcggc ttgttgcccg cttcgaaacc
    12121 aatgtctaaa gagaggttaa agccgacagc tgcagtttca tcaatcacca cgatgccatg
    12181 ttcatctgcc cagtcgagca tctcttcagc gtaagggtaa tgcgaggtac ggtaggagtt
    12241 ggccccaatc cagtccatta atgcgtgatc gtgcaccatc agcacgttat cgaatccttt
    12301 gcctcgcaaa tccgcatctt catgacgacc aaagccagta aagtagaacg gtttgtggtt
    12361 aatcaggaac tgttcgccct tcactgccac tgaccggatg ccgacgcgca gcgggtagat
    12421 atcacactct gtctggcttt tggctgtgac gcacagttca tagagataac cttcacccgg
    12481 ttgccagagg tgcggattca ccacttgcaa agtcccgctg gtgccttgtc cagttgcaac
    12541 cacctgttga tccgcatcac gcagttcaac gctgatatca ccattggcca ccacctgcca
    12601 gtcaacagac gcgtggttac agtcttgcgc gacatgcgtc accacggtga tatcgtccac
    12661 ccaggtgttc ggcgtggtgt agagcattac gctgcgatgg atcccggcat agttaaagaa
    12721 atcatggaag taagactgct ttttcttgcc gttttcgtcg gtaatcacca ttcccggcgg
    12781 gatagtctgc cagttcagtt cgttgttcac acaaacggtg atacgtacac ttttcccggc
    12841 aataacatac ggcgtgacat cggcttcaaa tggcgtatag ccgccctgat gctccatcac
    12901 ttcctgatta ttgacccaca ctttgccgta atgagtgacc gcatcgaaac gcagcacgat
    12961 acgctggcct gcccaacctt tcggtataaa gacttcgcgc tgataccaga cgttgcccac
    13021 ataattacga atatctgcat cggcgaactg atcgttaaaa ctgcctggca cagcaattgc
    13081 ccggctttct tgtaacgcgc tttcccacca acgctgatca attccacagt tttcgcgatc
    13141 cagactgaat gcccacaggc cgtcgagttt tttgatttca cgggttgggg tttctacagg
    13201 acgtaacata agggactcct cattaagata aaaatactgg tcaaccttta atctgattag
    13261 ttaaccaatt ctgtgttgca cacagttatc tgtgagaaac ccatggaaga gaaatggaaa
    13321 taaaaagaac tgacaccgcg atcaaaaaac caaaaactgc aatacccaca gcgtgatatg
    13381 gatcgcatta atctttaaaa cagcgttgac agaagccata aaataagcaa ccgaacgttc
    13441 gttcgagagt attgggaacg ataaactctc atgacatatg gctacggcga atattttggg
    13501 ggggaattct ccgaacacgg ggaggataat ttttgcacag agaaattacg atatgaaacg
    13561 cgggcacagt tatcaccagc ccgcgaaatg agaggatcag gatgcggtta atataccgcc
    13621 aatcatcgcg cgtaatccct gagcaaacga cgcttcacgc gccaggtctt ccgcttcgat
    13681 atcggccagt acaccgtagg tcagatccag cagtaaacgt gaagtcattg ccgtgttgat
    13741 gtctgggctt atctcgcctt tttgctgggc atcacgcatc cgctgggcaa caaattcagt
    13801 gatcgtcata tgcttatttt taagcatggc ggcgacctgt gggttacgca tcccttccgc
    13861 cataatttcg accaccagcg cccgttgccc aaaggcttca tgggtgagag agacaataga
    13921 ctcgaccata tagtcaacaa aatgaatccc ttcaatcggt tcccggaaac gagccagcgc
    13981 cctctcctgg tcctgtaaga taatcgcctg aatcaaggct tctttggaga tgaaatggtg
    14041 atagagcgtc ccaggactaa tggcgcaaga tttacagatg gctttcatcg aggcactgtg
    14101 aaatccattt tctgaaaaaa tctctctggc agcattgagg atccgggtcc gtgtcggttg
    14161 tgcttcagtc tgcatgttat ccatcattaa accattatag ttagcgtgaa taactagttt
    14221 accgcgttgt caggtgagcg cctatcattg ctgttgaata gtgcgaaggt acactctatt
    14281 cgtatacttt tcacccagca attggttacc caatagccgt gatttggctg ccagttgttt
    14341 agcagagacg ttgatgaatt gcacacgcta tcccggtaaa caacacatac gaccgtttac
    14401 cggttcgtta gtgtattaat tgagctcctg taccccacca ccggagacgg tgagaatttg
    14461 tccgcttacc cagctggctg caggcgagca aaggaacagc gccgcgttag caatatcttg
    14521 cggttggccc agacgtctga tcggcgtgtg ctgtaacatt ttttgttcaa tttctggtgt
    14581 aataacggat ttcagggcat cggttaatat tgcccccggc gcaatgccat tcacccgaat
    14641 atttttttca cccaggtcaa atgccatatt tctgaccaga tgactggccg cagctttaga
    14701 tgatgcatag gaagtcatgt ttatattttt attttctgcc gccatagaag tgatggtcag
    14761 aataacgcca ccgccatttt tttccatttc tggcgcaaca agttgtgaca gatggaaaaa
    14821 agaaaacaca ttcagttcat aagcacggcg aaaatctgcc attggcatat caaacggttt
    14881 aggaccaccg ccaccggcgt tgttaaccag gatatcaact ttacccagtt tactgacggc
    14941 aaagtctgcc agtgcagaga gttcctgttc ggaagtaata tcacaacgcc aggcaaatgc
    15001 ctgaccaccc agttgttgaa tttcgtctac aacatggtta gctgcgtcgg cgttaatatc
    15061 actgaccacc acagatgcgc cagctgtcgc gaatgtaatg gcgatttctt taccaatacc
    15121 tgcacccgca cctgtgatga tggcgcattt tccgtcgagt ctcaggttgt cagaattaaa
    15181 cacataaacc tcctgtgaac ttcagtaaaa ataaaaggca ccgcctg
//
LOCUS       DADLSA010000093        15205 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.093, whole genome shotgun sequence.
ACCESSION   DADLSA010000093 DADLSA010000000
VERSION     DADLSA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.093"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            455..2734
                     /gene="ydeP"
                     /locus_tag="JN691_002803"
     CDS             455..2734
                     /gene="ydeP"
                     /locus_tag="JN691_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAY7244208.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2982..3179
                     /gene="safA"
                     /locus_tag="JN691_002804"
     CDS             2982..3179
                     /gene="safA"
                     /locus_tag="JN691_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HAY7244209.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3253..4014
                     /gene="ydeO"
                     /locus_tag="JN691_002805"
     CDS             3253..4014
                     /gene="ydeO"
                     /locus_tag="JN691_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAY7244210.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4416..6098
                     /locus_tag="JN691_002806"
     CDS             4416..6098
                     /locus_tag="JN691_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY7244211.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            6150..7307
                     /locus_tag="JN691_002807"
     CDS             6150..7307
                     /locus_tag="JN691_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY7244212.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(7273..7395)
                     /locus_tag="JN691_002808"
     CDS             complement(7273..7395)
                     /locus_tag="JN691_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244213.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            7604..9289
                     /locus_tag="JN691_002809"
     CDS             7604..9289
                     /locus_tag="JN691_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HAY7244214.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGIWNLADDICDISTVL"
     gene            9327..11699
                     /locus_tag="JN691_002810"
     CDS             9327..11699
                     /locus_tag="JN691_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY7244215.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANDSDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            11744..14539
                     /locus_tag="JN691_002811"
     CDS             11744..14539
                     /locus_tag="JN691_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY7244216.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGL
                     RYMIYPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETF
                     ESMGLRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAE
                     RGVITEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQR
                     WYQPNNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDK
                     ENRVNGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTA
                     RSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWL
                     KNAADQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQL
                     RKNQDAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQ
                     AEISSKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIA
                     LANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLIN
                     QRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIA
                     QFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPL
                     TRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIRE
                     QASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLTKGISEQEL
                     NEYQQNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVK
                     QYLSHPVNTYTGVLLPK"
     gene            14901..>15205
                     /locus_tag="JN691_002812"
     CDS             14901..>15205
                     /locus_tag="JN691_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7244217.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT         4462 a         3316 c         3307 g         4120 t
ORIGIN      
        1 ggggacagta aataaaagca aagtacctct gggtttaagc gcaacctatg gacaaacagg
       61 aaacaaggtt tcagccggaa ccgtccagtc agtcattggt gtcactttta tctatgaata
      121 attgtgattt tgaccttagt ctatgcgctc cattcacgaa aattgcgcgg ataattattt
      181 tgtgaaggct attatcctac acctgtaaga aaatccgcgc acaattgaag ccagagtctt
      241 attttttata ttgaaaaata tgttaattta aacactatga gagatgatgc ttaacttatc
      301 gtatttaatc tattgtttaa cgataataga ttaaaaatag aaagagagta gtcccctact
      361 cttatatatc catgttggcg ataatccctt tgtatgtact gtgcatcatc gctattacaa
      421 atcctaataa ttcatttcca cacaggataa gtagatgaag aaaaaaattg aatcctacca
      481 gggtgctgca ggtggttggg gtgctgttaa atccgtagcg aatgcagtac gtaagcagat
      541 ggatatacgc catgatgtta ttgccatgtt tgacatgaat aagccagagg gctttgactg
      601 tccgggttgt gcatggccag atcctaagca cagtgcgtca ttcgacattt gtgaaaacgg
      661 cgcaaaagca atcgcctggg aagtcacgga taagcaggta aatgcctctt tctttgctga
      721 gaatacggtt caatcattac ttacctgggg agatcacgag cttgaggctg cggggcgtct
      781 cactcagcct ttgaaatatg atgccgtcag cgactgttac aagccattaa gctggcaaca
      841 agctttcgac gaaattggcg cacgccttca aagctatagt gatcccaatc aggttgaatt
      901 ctatacttcg ggccgcactt ccaatgaagc tgcctttctt tatcagcttt ttgcccgtga
      961 atacgggagc aataactttc ccgactgctc caacatgtgc catgaaccga caagcgtggg
     1021 tttggcagcg agtatcggtg taggtaaagg gaccgtgttg ctggaagact ttgagaagtg
     1081 cgatttagtc atttgcattg ggcataaccc tggtacaaac caccctcgca tgctgacttc
     1141 gttgcgcgct ttagtgaaac ggggagcgaa aatgatcgcc atcaatcctc tacaggaacg
     1201 tggcctggag cgatttaccg caccgcaaaa cccgtttgaa atgctgacga actctgagac
     1261 tcagttggcc agtgcctact acaacgtgcg cattggtggc gatatggcgt tgctcaaggg
     1321 gatgatgcgc ctgttaattg agcgcgatga tgctgcaagt gccgcaggtc ggccctcatt
     1381 gcttgatgac gaatttattc aaacgcatac cgtcggcttt gacgagctac gccgtgacgt
     1441 tctcaattcc gagtggaaag atatcgaacg tatttctgga ctaagtcaga cacaaatcgc
     1501 cgaactggct gacgcatatg ccgctgccga acgcaccatt atctgttacg gaatggggat
     1561 cactcagcac gaacatggta cccagaacgt gcagcaactg gtcaatttgc tgttgatgaa
     1621 aggtaacatt ggcaagcctg gtgcgggtat ctgcccacta cgtggtcact ctaatgtaca
     1681 gggcgaccga accgtcggta tcaccgagaa accgtctgca gagtttctgg ctcgcctggg
     1741 tgagcgctat ggcttccccc cacctcatgc acctggacat gctgcaattg ccagcatgca
     1801 agcaatatgt acggggcagg ctcgagcatt gatctgcatg gggggcaact ttgcactggc
     1861 aatgccagat cgggaagcga gcgctgtacc gttaacgcaa ttagatttgg cggtacacgt
     1921 agccactaag cttaaccgct ctcatctgct gaccgcacgg catagctata ttctgccggt
     1981 tctggggcgt agcgagattg atatgcaaaa aagcggtgcg caggcggtaa ccgttgagga
     2041 ttcaatgtcg atgattcatg cctcgcgtgg cgtgttaaaa cccgccggtg taatgctgaa
     2101 atcagagtgt gcagtggtcg cgggaatcgc gcaggcagca ctaccccaga gcgtggtagc
     2161 ctgggagtat ctggtggaag attatgatcg cattcgcaat gacattgaag ctgtgctgcc
     2221 agagttcgcc gactataacc agcgcatccg tcatcccggt ggttttcacc tgataaatgc
     2281 agctgctgaa aggcgctgga tgacgccgtc aggtaaggct aatttcatta ccagcaaagg
     2341 gctgttagaa gatccctctt cagcgtttaa cagtaagctg gtcatggcga cagtacgcag
     2401 ccacgatcag tacaacacga cgatttatgg tatggatgat cgctatcgag gggtattcgg
     2461 tcaacgagat gtggtcttta tgagtgctaa acaagctaaa atttgccgtg taaaaaacgg
     2521 cgaaagagtt aatcttattg cgcttacgcc agacggtaag cgcagctcac gccgcatgga
     2581 tagattaaaa gtggtcattt accctatggc tgaccgctca ctggtgacct attttccaga
     2641 atcgaatcac atgctaacac ttgataacca cgatccatta agtggcattc ctggctataa
     2701 aagtattccg gttgaattag aaccatcaaa ttaatgcctc atctcatttc ttctgctgtc
     2761 atccgcacag cagaagaatt cctcattgac tattattgcg caatttgctc acatggatta
     2821 aattaaacta catactataa gatataaact tctgcctaca gctgtaagaa actccgctca
     2881 gtactgaagc accagtccta tttcttcttt tctccagcct gttatattaa gcatactgat
     2941 taacgacttt taacgttatc cgctaaataa acatatttga aatgcatgcg accacagtga
     3001 aaaacaaaat cacgcaaaga gacaactata aagaaatcat gtctgcaatt gtggttgtct
     3061 tattactgac acttacgttg atagccattt tttcggcaat tgatcagctg agtatttcag
     3121 aaatgggtcg cattgcaaga gatcttacac atttcattat caatagtttg caagactgaa
     3181 aataaacagc aaattataaa tgtgaaatgt ttaaaaggta tcgataaaac tttattgttt
     3241 taaggagata aaatgtcgct cgtttgttct gttatattta ttcatcatgc tttcaacgct
     3301 aacattttag ataaagatta cgccttctct gacggcgaga tcctgatggt agataacgct
     3361 gttcgtacac attttgaacc ttatgagcgg cattttaaag aaatcggatt taatgaaaat
     3421 accattaaaa aatatctaca atgcactaac atccagacag tgacggtgcc tgttcctgcg
     3481 aagtttttac gtgcttcaaa tgtaccgact ggattgctta atgaaatgat tgcttatctc
     3541 aactcggaag aacgcaatca tcataatttt tcagaacttt tgcttttttc ttgcctgtct
     3601 atttttgccg catgcaaagg tttcattaca ctattaacta acggtgtgct atccgtttcc
     3661 gggaaagtga gaaatattgt caacatgaag ctggcgcacc catggaagct gaaagatatt
     3721 tgtgactgcc tgtacatcag tgaaagcctg ttgaagaaaa aactaaagca agagcaaacg
     3781 acattctcac agattctatt agatgccaga atgcagcacg caaaaaattt gatacgcgta
     3841 gaaggttcag tcaataaaat tgccgaacaa tgtggttatg ccagtacatc ttattttatt
     3901 tatgcgttcc gcaaacattt cggcaacagt ccgaagagag tttctaagga gtaccgttgt
     3961 caaaggcaca cgggtatgaa tacgggcaac acgatgagtg ctttagctat ttgattattt
     4021 gctaacgagt agtcaaccac gcacgctacg taagaattat atggggcagc cattccctgc
     4081 cccgcgttgt ttttaggcga tatatttatt gaaataaata agtgacatcc atcacatatt
     4141 tatgcacttg cataacctgt tgcatgatta tttatgatct caattcggca ttttgtcagt
     4201 aaaatgcaat aatttattaa atatcaataa attagttgtt tatcggcgag aaattactta
     4261 atagaacaga aagtaatgtc aacgctttat ggactgttat ttcccttttt ttagctaaat
     4321 ctgctatctc tttatgtgac taacttcact tacatccact tatttctctt cgtaaaatta
     4381 cttcggaatt aagtacaata agaagaggaa catttatgaa gtctgcatta aagaaaagtg
     4441 tcgtaagtac ctcgatatct ttgatactgg catctggtat ggctgcattt gctgctcatg
     4501 cggcagatga tgtaaagctg aaagcaacca aaacaaacgt tgctttctca gactttacgc
     4561 cgactgaata cagtaccaaa ggaaagccaa atattatcgt actaaccatg gatgatcttg
     4621 gttatggaca acttcctttt gataagggat cttttgaccc aaaaacaatg gaaaatcgtg
     4681 aagttgtcga tacctacaaa atagggatag ataaagccat tgaagctgca caaaaatcaa
     4741 cgccgacgct cctttcatta atggatgaag gcgtacgttt tactaacggc tatgtggcac
     4801 acggtgtttc cggcccctcc cgtgccgcaa taatgaccgg tcgagctccc gcccgctttg
     4861 gtgtctattc caataccgat gctcaggatg gtattccgct aacagaaact ttcttgcctg
     4921 aattattcca gaatcatggt tattacactg cagcagtagg taaatggcac ttgtcaaaaa
     4981 tcagtaatgt gccggtaccg gaagataaac aaacgcgtga ctatcatgac aacttcacca
     5041 cattttctgc ggaagaatgg caacctcaaa accgtggctt tgattacttt atgggattcc
     5101 acgctgcagg aacggcatat tacaactccc cttcactgtt caaaaatcgt gaacgtgtcc
     5161 ccgcaaaagg ttatatcagc gatcagttaa ccgatgaggc aattggcgtt gttgatcgtg
     5221 ccaaaacact tgaccagcct tttatgcttt acctggctta taatgctccg cacctaccaa
     5281 atgataatcc tgcaccggat caatatcaga agcaatttaa taccggtagt caaacagcag
     5341 ataactacta cgcttccgtt tattctgttg atcagggtgt aaaacgcatt ctcgaacaac
     5401 tgaagaaaaa cggacagtat gacaatacaa ttattctctt tacctccgat aatggtgcgg
     5461 ttatcgatgg tcctctgccg ctgaacgggg cgcaaaaagg ctataagagt cagacctatc
     5521 ctggcggtac tcacacccca atgtttatgt ggtggaaagg aaaacttcaa cccggtaatt
     5581 atgacaagct gatttccgca atggatttct acccaacagc tcttgatgca gccgatatca
     5641 gcattccaaa agaccttaag ctggatggtg tttccttgct gccctggttg caagataaga
     5701 aacaaggcga gccacataaa aatctgacct ggataacctc ttattctcac tggtttgatg
     5761 aggaaaatat tccattctgg gataattacc acaaatttgt tcgccatcag tcagacgatt
     5821 acccgcataa ccccaacact gaggacttaa gccaattctc ttatacggtg agaaataacg
     5881 attattcgct tgtctataca gtagaaaaca atcagttagg tctctacaaa ctgacggatc
     5941 tacagcaaaa agataacctt gccgccgcca atccgcaggt cgttaaagag atgcaaggcg
     6001 tggtaagaga gtttatcgac agcagccagc caccgcttag cgaggtaaat caggagaagt
     6061 ttaacaatat caagaaagca ctaagcgaag cgaaataact aaaccttcat gcggcggatt
     6121 tttccgccgc cttattgagc gagatagcga tgcacgttac agccaagccc tccagttttc
     6181 aatgtaatct caaatgtgat tactgttttt accttgaaaa agagtcgcag tttactcatg
     6241 aaaaatggat ggatgacagc actctgaaag agttcatcaa acaatatatc gaagcgtctg
     6301 gcaatcaggt ctattttacc tggcaaggcg gtgaacccac tctggctggc ctggattttt
     6361 tccgtaaagt tattcactat caacaacgct atgcaggcca aaaacgtatt tttaatgcat
     6421 tacaaacgaa tggcatttta ttgaataatg aatggtgtgc ctttctcaaa gaacatgaat
     6481 ttctggttgg tatctcgatc gatggccccc aggagttaca tgaccgttac agacgcagta
     6541 attcaggtaa cggtactttt gcaaaagtga tagcagccat cgagcgtctg aaatcatatc
     6601 aagtagagtt taatacgtta accgtcatta ataacgttaa tgtccattac cctcttgagg
     6661 tttatcattt tttaaaatct atcggcagta aacatatgca atttatcgaa ttgctagaaa
     6721 ccgggacgcc gaatattgat ttcagtggtc atagtgagaa cacattccgt atcattgatt
     6781 tttctgtgcc tcccacggct tatggcaagt ttatgtcaac catttttatg caatgggtta
     6841 aaaacgatgt gggtgaaatt ttcattcgtc agtttgaaag ctttgtcagc cgttttttgg
     6901 ggaatgggca tacaagttgt attttccagg agtcctgcaa ggataatctg gttgttgaaa
     6961 gtaatggaga catttacgaa tgcgaccatt ttgtctatcc acagtacaaa attggaaaca
     7021 ttaataaatc tgaactcaaa acgatgaaca gtgtacaact gacagcgcaa aaaaaacgga
     7081 tttcagcgaa atgtcagcaa tgtgcatata aacctatctg caatggcggt tgtcctaagc
     7141 atcgtattac taaagtaaac aatgagactg tttcttattt ttgcgaaggt tataaaatcc
     7201 ttttttcaac catggtacct tatatgaacg ccatggttga gttagctaag aacagagtac
     7261 cgctttacca cattatggat gttgcaaaac aaatggagaa taattaataa cgctctcttt
     7321 taactccatt cttagcctcc ttcttatgac cttaacaagg cacccgacat aaattcctgt
     7381 cgggtgtttc gtcatcatca tcttcccttg cgaaaatcct ggcttacgtg gtatgcgtca
     7441 gggcattagc aatacccttg attatcaata gcataaccat aaccataacc actgaaaatt
     7501 aaaaatttct atatatttca tattgttaaa tgatacttaa ataaatttca tcagtaaatc
     7561 attgccatag ataatcattg gatggcacaa tcaattttcg ctaatgataa ccattcccat
     7621 tacgttttgt atgttgatag caaagtatct ctgtctgctg aagccctttt ggctgcgtaa
     7681 gaataataaa acttcagtgt tgttaatcat tattatcctc gcaatgatcc ttggcgtggt
     7741 taaaattcag gtctggttaa acgactggaa taatgatttt ttcaatgcgc taagccagaa
     7801 agaaaccgat aagctttggc agcttgttct ttggttccct gccctgctgg ggatctttgt
     7861 attaatatcc gtaaacaaaa catggttaat taaactactg actattcgct ggcgcgaatg
     7921 gttaacagat tactacctta atcgctggtt tgcagataaa aactactact tcacgcagat
     7981 ctacggcgag cataaaaata cagataaccc tgaccaacgt atcgctgaag acattctctt
     8041 actgatcagc aaaacgctca gtctttcatt tggcttcatc cagtcactta gcatgctgat
     8101 cacctttacc gttattcttt ggcaaagcgc gggtacgctc tcattcactg ttggtggaac
     8161 agaatggaat atccaggggt atatggtcta taccgtcgtg ctcattgtga tcggtggaac
     8221 tttatttacc cataaggtgg gtaaacgtat tcgtccgctt aatgtggaaa aacaacgtag
     8281 cgaagcgaca tttcgaacta atcttgtgca gcataataag caggcagaac tgattgcgct
     8341 aagcaatgct gagtctctac aacgccagga gttgcgcgac aattttcata ccattaaaga
     8401 gaactggcat cgtttaatga atcgtcagcg gtggcttgat tactggcaaa atatttattc
     8461 gcgctcgctt agcgttcttc cctactttct gttattgccg cagtttatta gcgggcagat
     8521 caatctgggc ggactgatga aatcgcgcca ggcatttatg ctagtatcga acaatttaag
     8581 ctggtttatt tataaatatg acgaacttgc tgaactggct gcggttatcg atcgcttgta
     8641 tgagttccat caactcactg aacagcgccc tacgaataag cctaaaaatt gccaacatgc
     8701 ggtacaagtg gctaatgcga gtattcgtac gcctgataat aagatcatat tagagaactt
     8761 gaactttcat gtttcgccag gcaaatggct attactgaaa ggctactctg gtgcgggaaa
     8821 aaccacactg cttaaaacat tatcccactg ctggccgtgg tttaaaggtg atatttcttc
     8881 tcctgctgac agttggtatg tgtcacaaac accgttaatc aaaagcggct tactgaaaga
     8941 gattatttgt aaagcacttc ccctgcccgt agacgataaa tcgttgagcg aagtactgca
     9001 tcaggttggt ctggggaaat tggctgcgcg tattcatgac cacgatcgct ggggagatat
     9061 tctttccagc ggcgaaaaac aacgtatcgc cctggcacga ttaattttac gacgtccgaa
     9121 atggatattt cttgacgaaa ctacctctca tcttgaggaa caagaggcta tacgcttact
     9181 gcgtttagtg cgtgaaaaac tacccacaag cggcgtcatt atggttacac atcaacccgg
     9241 tatctggaac ctggctgatg atatttgtga cattagcacg gttttataac aacaataaaa
     9301 gccctgctgt acggagatat aaataaatga agcgagttct tattcctggc gtcattttat
     9361 gtggcgctga tgtggcgcag gccgtcgatg acaaaaacat gtacatgcat ttttttgaag
     9421 agatgacggt ctatgctcct gtccctgtac ccgtaaacgg caacacgcat tacaccagtg
     9481 aaagcatcga gcgtttaccg accgggaatg gcaatatcag cgatctgctg agaaccaacc
     9541 ctgcggtacg catggattca acgcaaagta cctcgttgaa ccagggagat attcgccctg
     9601 agaaaatctc tattcacggt gcgtcgccct accagaatgc ctatttgatt gacggtatta
     9661 gtgcaactaa taacctgaac ccagcgaatg attccgatgc cagtagtgca accaatatta
     9721 gcgggatgtc acaggggtat tatcttgatg tcagcttact ggacaatgtg acgctttatg
     9781 acagttttgt gccggttgaa tttggtcgct tcaatggcgg ggtaattgat gcaaagatca
     9841 aacgcttcaa cgctgatgat agcaaggtga aattgggtta tcgcactacg cgtttggact
     9901 ggttaacatc gcatatcgat gagaataaca agagcgcatt taatcaaggt tcttcaggaa
     9961 gtacctattt ctctccagat tttaaaaaga acttttatac cttgtcgttt aatcaggagc
    10021 tggctgataa ctttggcgtt accgccggtt tatcgcgccg ccagtctgat atcacccgcg
    10081 cggattatgt ttcgaatgac ggcattgtcg ccggtcgggc acagtataaa aacgttatcg
    10141 atactgcatt gagcaaattt acctggtttg ccagcgaccg ctttacccac gatttaacct
    10201 taaaatatac cggctccagc cgtgattata ataccagcac cttcccgcag tctgatcgcg
    10261 aaatgggtaa taaatcctat ggtctggcat gggatatgga tacgcaactc gcatgggcca
    10321 aactacgtac caccgttggt tgggatcata ttagtgatta tacccgtcac gatcatgaca
    10381 tctggtacac cgaactttca tgtacatatg gtgatattac agggcgttgt acccgtggcg
    10441 gattaggaca catttcccag gctgtagata attacacctt caaaacacgc ctggactggc
    10501 aaaaattcgc cgtgggtgat gtttcgcatc aaccctactt cggcgcggaa tacatctatt
    10561 ccgatgcatg gactgaacgc cataaccagt ctgaatccta tgtgattaat gctgcaggaa
    10621 agaaaactaa ccataccatt taccataaag gtaaaggcag cctgggaatt gacaactaca
    10681 cgctgtatat ggcggatcac attagctggc ggaatgtgtc gttaatgccc ggtgtgcgtt
    10741 atgactatga caactatctg tcaaaccaca atatctctcc gcgctttatg acggaatggg
    10801 atatttttgc tgatcaaacc tcaatgatta ccgccggtta taaccgttac tatggcggga
    10861 atattcttga tatgggatta cgtgatatcc gcaatagctg gacggaatcg gtatcaggta
    10921 ataaaaccct gacgcgttat cagaatttga aaacgcctta taacgatgaa ctggcaatgg
    10981 gattgcagca aaaaatcgat aagaacgtta ttgcacgcgc aaactatgtt taccgtgaag
    11041 cgcatgatca aatcagcaaa agcagtcgta ccgacagcgc gactaaaacc accattactg
    11101 aatataacaa cgatggcaaa accaaaacgc attcgtttaa cctcagtttt gaactggccg
    11161 aacccctgca tatccgccag gtagatatta acccgcaaat tgtctttagc tatatcaaga
    11221 gcaagggcaa cttgtcgtta aacaatggtt atgaggagag caataccggt gataaccagg
    11281 tggtttataa cggtaatctg gtctcttacg atagcgttcc agtggcagat tttaataacc
    11341 cattaaagat ctccttaaac atggatttca cgcatcaacc gagcgggtta gtgtgggcga
    11401 atacgctggc ctggcaagaa gcgcgtaaag ctcgcattat ccttggtaag acaaatgcgc
    11461 aatacatcag cgaatattca gattacaagc agtatgttga cgaaaaactg gatagcagcc
    11521 tgacctggga cacccgcttg tcctggacgc cacaatttct gaaacaacaa aacctgacga
    11581 tcagtgccga tattctcaat gtactggata gcaaaaccgc tgttgataca acgaacaccg
    11641 gtgtggcgac ctacgccagt ggccgtactt tctggcttga tgtcagcatg aaattttaat
    11701 agttcggatt ttacttaacc gggtaaattc gcccggtttt cgcatggaga taattatgag
    11761 aaacctctgt ttcttactga cgttagtggc aactctgttg ctccccgggc ggctgattgc
    11821 cgccgcctta ccgcaggatg aaaagttaat taccgggcaa ctggacaatg gcttgcgata
    11881 tatgatttat ccgcatgctc atccaaagga tcaggtaaat ttatggctgc aaattcatac
    11941 cggttcattg caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat
    12001 gtttaacggc acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg
    12061 cctgcgtttt ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt
    12121 gagtttgccg actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg
    12181 gagtaatgcc gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga
    12241 ggaatggcgt gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct
    12301 gctggctaat acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac
    12361 ggtcacaccg gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac
    12421 ctttatcgtg gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt
    12481 aagtaagctt ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa
    12541 ccacctgcgc tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta
    12601 ttatcgcctg ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg
    12661 gagcatgtta gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt
    12721 gaagactatt tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct
    12781 gtttttccgt gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc
    12841 agagttggca accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc
    12901 tacccgcctc acctggctga aaaatgcggc tgatcagcaa gctgaacgtg atttacgtat
    12961 gctgaccagt cgcctagcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga
    13021 gacatatcaa ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa
    13081 atggcagcag ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct
    13141 tgccgccaaa aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa
    13201 caaaaagctg gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga
    13261 cccacaggcg gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact
    13321 ttccaatggt gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat
    13381 tattgccgta tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc
    13441 gctggcaaat aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa
    13501 acgctggagt gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt
    13561 gctctccgtt agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg
    13621 aatcacccac agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca
    13681 ggcgttgaaa acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac
    13741 gcgctatgct gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc
    13801 cgcagatgcg ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt
    13861 cattgtcggt aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc
    13921 aatcaaacac tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa
    13981 cgcatcggtt actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg
    14041 ttatgattcc cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt
    14101 cgcactggca aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt
    14161 ttcttctcgc ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac
    14221 ttgtcaacca gaacgacatg atgaactgtt aacgttagcg aatgaagtga tggttaagcg
    14281 tttgactaag gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag
    14341 cctcgatatc caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat
    14401 tcaatatgac gatcctgcag catggactga gcaggagcaa ttgttgaaac aaatgacggt
    14461 agagaatgtt aacactgccg ttaaacaata tctttctcat ccggtaaata cttataccgg
    14521 agtattattg ccaaaataat aacagccccg tcaacacatc gttggcgggg attttagcaa
    14581 tattcgctat ttttatgtaa taattttata aatgcgttca aaataataac caagtactaa
    14641 tagtgatatt ttaatgtctg atttttacgt gataattcag gaggcacaga aagcgcataa
    14701 aaataacaac ataaaacacc ttgccaccac ccaagaattc tctattgcat tgttttttaa
    14761 tgaaaaaata tcattccaac aatatctcac gataaataac attaggattt tgttatttaa
    14821 acacgagtcc tttgcacttg cttactttat cgataaatcc tactttttta atgcgatcca
    14881 atcattttaa ggagtttaaa atggataaga agcaagtaac ggatttaagg tcggaactac
    14941 tcgattcacg ttttggtgcg aagtctattt ccactatcgc agaatcaaaa cgttttccgc
    15001 tgcacgaaat gcgcgacgat gtcgcatttc agattatcaa tgatgaatta tatcttgatg
    15061 gcaacgctcg tcagaacctg gccactttct gccagacctg ggacgacgaa aacgtccaca
    15121 agttgatgga tttatccatt aacaaaaact ggatcgacaa agaagaatat ccgcaatccg
    15181 cagccatcga cctgcgttgc gtaaa
//
LOCUS       DADLSA010000094        15049 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.094, whole genome shotgun sequence.
ACCESSION   DADLSA010000094 DADLSA010000000
VERSION     DADLSA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.094"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..238
                     /locus_tag="JN691_002813"
     CDS             <1..238
                     /locus_tag="JN691_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAY7244218.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="JN691_002814"
     CDS             248..886
                     /locus_tag="JN691_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAY7244219.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNSYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="JN691_002815"
     CDS             889..2052
                     /locus_tag="JN691_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY7244220.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..3761
                     /locus_tag="JN691_002816"
     CDS             2136..3761
                     /locus_tag="JN691_002816"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HAY7244221.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASSPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3878..4153)
                     /gene="yjfN"
                     /locus_tag="JN691_002817"
     CDS             complement(3878..4153)
                     /gene="yjfN"
                     /locus_tag="JN691_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAY7244222.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4302..4631)
                     /gene="bsmA"
                     /locus_tag="JN691_002818"
     CDS             complement(4302..4631)
                     /gene="bsmA"
                     /locus_tag="JN691_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAY7244223.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4813..5562
                     /gene="yjfP"
                     /locus_tag="JN691_002819"
     CDS             4813..5562
                     /gene="yjfP"
                     /locus_tag="JN691_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7244224.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGALMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5559..6314)
                     /gene="ulaR"
                     /locus_tag="JN691_002820"
     CDS             complement(5559..6314)
                     /gene="ulaR"
                     /locus_tag="JN691_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAY7244225.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6422..7486)
                     /gene="ulaG"
                     /locus_tag="JN691_002821"
     CDS             complement(6422..7486)
                     /gene="ulaG"
                     /locus_tag="JN691_002821"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAY7244226.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGKALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7841..9238
                     /gene="ulaA"
                     /locus_tag="JN691_002822"
     CDS             7841..9238
                     /gene="ulaA"
                     /locus_tag="JN691_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAY7244227.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIISFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDNYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9254..9559
                     /gene="ulaB"
                     /locus_tag="JN691_002823"
     CDS             9254..9559
                     /gene="ulaB"
                     /locus_tag="JN691_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAY7244228.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9569..10033
                     /gene="ulaC"
                     /locus_tag="JN691_002824"
     CDS             9569..10033
                     /gene="ulaC"
                     /locus_tag="JN691_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HAY7244229.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10047..10697
                     /gene="ulaD"
                     /locus_tag="JN691_002825"
     CDS             10047..10697
                     /gene="ulaD"
                     /locus_tag="JN691_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAY7244230.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10707..11561
                     /locus_tag="JN691_002826"
     CDS             10707..11561
                     /locus_tag="JN691_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAY7244231.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ECLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            11561..12247
                     /locus_tag="JN691_002827"
     CDS             11561..12247
                     /locus_tag="JN691_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HAY7244232.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12376..12651)
                     /locus_tag="JN691_002828"
     CDS             complement(12376..12651)
                     /locus_tag="JN691_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7244233.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12978..13373
                     /gene="rpsF"
                     /locus_tag="JN691_002829"
     CDS             12978..13373
                     /gene="rpsF"
                     /locus_tag="JN691_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAY7244234.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTVAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13380..13694
                     /gene="priB"
                     /locus_tag="JN691_002830"
     CDS             13380..13694
                     /gene="priB"
                     /locus_tag="JN691_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAY7244235.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13699..13926
                     /gene="rpsR"
                     /locus_tag="JN691_002831"
     CDS             13699..13926
                     /gene="rpsR"
                     /locus_tag="JN691_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAY7244236.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13968..14417
                     /gene="rplI"
                     /locus_tag="JN691_002832"
     CDS             13968..14417
                     /gene="rplI"
                     /locus_tag="JN691_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAY7244237.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEVRRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14488..15045)
                     /locus_tag="JN691_002833"
     CDS             complement(14488..15045)
                     /locus_tag="JN691_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7244238.1"
                     /translation="MNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDVE
                     SFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQLR
                     TVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSD
                     RMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3512 a         3659 c         4066 g         3812 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatagct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacgttttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaacc gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcacgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccaata acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgcaaacgag
     3301 gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat tgcgaggaga
     3361 gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa ggttccggca
     3421 atattatgtg cctggatgtg ttgcgcgttc tcaataagca agcgggcgta tacgacttat
     3481 tgtcggaagc atttgtggaa gtgaaagggc aggatcgcta ttttgatcgc gcggttcgtc
     3541 gtttacagca gcagctgcgt aagccagctg aagaactggg gcgagagatt actcatcagc
     3601 tattcctgct gggctgcggt gcgcaaatgt tgaaatatgc ttcttcgcca atggcgcagg
     3661 cgtggtgtca ggtgatgtta gatacgcgcg gcggcgtacg gttgtcagag cagatccaga
     3721 atgatttatt gctgcgggcg acggggggag tgtgtgtgta agcgtatacg actgatgcga
     3781 cgctggtttc gattaactaa atgaaatatg tgaaaattgt aggccggaca aggcgctcgc
     3841 gccgcatccg gcattgttca tttgctgtag atgacactca tgcatacagt atcgcctgta
     3901 cgcgccagtt atcaggctgc tggtcttcgt acatggtaat aatgcggtac catgacgcac
     3961 cttgttcatc ggcctttaac gccacgatac gttccacgtc ctgcggatcc cctgaaatat
     4021 tgctaactga gatctgacct atttcattca ggcccgtcac gcagtcagca ctggcgaatt
     4081 ctgcagactg tgccgtggcg ctcgcaggat aagtcactaa ttgcagcgag ggtgaggcaa
     4141 gtaattgttt catcgtcagc tccatttttc gtttccccac atgttgcagg gcattccatc
     4201 gcggtaataa acgcccaagc ttccagccgg taatttattg tgcacaggta aacgcatagg
     4261 gacgcaaagc aatgtaaagc tgactatgta agttgtctga tttatttacg gtacaaaatg
     4321 gcttgtgaat accactgtcc tgtcacgatg gtttcgtcca ccatgacgac aacgtaataa
     4381 tcagcttttg cagcgacagc ttttgctctg atttctgcta atgcgtcatc cggagaaccc
     4441 cgaaccatcg tgcttacgct acctattcgc tgtaaccctt gcgtctggtc gcggcgaatc
     4501 tcttgcggat ggtccgttac tggcggtgct ggctgtggcg taccttgcag tgcgctacag
     4561 gcacttaaca tcaacaccaa taataaactg gcaaaccggt aaataacgct attacgtttc
     4621 ctgctaacca tagtgtagtg ccttattaat ttaactttgg gggaatgttc ccgaattgtt
     4681 acctggtacg cgattttcga atgaaaacgt gacgaaagcg taacccacat ctcaacatta
     4741 tgaactaata ccgccagcag aaacttgtgc taaggatttc ttcatgtcat accagacaaa
     4801 aaggagagac agatgattga aatagaatca cgcgagctgg cagatattcc cgttcttcat
     4861 gcttatcctg tcgggcaaaa agataccccg ttaccgtgcg taatttttta tcacggcttt
     4921 acttcatcca gcctggtgta tagctatttt gccgttgcgc tggcgcaggc tggtttgcgg
     4981 gtgatcatgc cggatgcgcc cgatcacggt agccgtttta gtggcgacga agcgcggcgg
     5041 ttaaatcaat tctggcaaat cttgctacaa agtatgcagg aattcactac tttacgtgcg
     5101 gcaatagccg aagaaaactg gctgcttgat gaccgtctgg cagtcggtgg cgcgttgatg
     5161 ggcgcgatga cggcactggg aattaccgct cgccacccca cggtgaaatg taccgccagc
     5221 atgatgggat cgggctattt tacatcgctc gcccgttcac tgtttccacc gctgatacct
     5281 gaaacggcag cacagcagaa tgaattcaat aaaattgtcg cgccgctggc agagtgggaa
     5341 gcgacaaacc acctggaaca acttggggac agacctctac tgctgtggca tggcctcgac
     5401 gatgacgttg tgcctgccga cgaatcacta cgtttgcagc aggccttaag cgagacggga
     5461 cgggataaac tgctaacctg ttcatggcag ccaggcgtgc gtcaccgcat tacgcctgag
     5521 gcgttagatg ctgccgtgac atttttccgc cagcatcttt aaacacgcag aatgctgacc
     5581 ccttgcgctt ccagttgttg caggatttcc gggttagcat ttttgccggt gatcagcata
     5641 tcgatttgat cggcacggct aaaaagcatt cccgcgcgtt cgccaatctt actgctatca
     5701 accagtacca ccagtttccc taccacgctc agcatcttct gctctgccat tgctgtcagc
     5761 atatcggttt tatacaaccc ttctgcggtc agcccttttc cgctggtaaa catccagtgc
     5821 ccggcataga gactgttttc gctgccctgc gggcttaaag tgatggactg acttttgttg
     5881 tactgtccac ccataatgat cacgctgtca tgttcttgat cgatcaggta atttgccagc
     5941 ggtagataat tagtgatgat ttgcactggc ttgccacaca tttcccgccc aagcagaaac
     6001 gcggtggagc cgcagttgat gactacgctt tcgcccggat taaccagctg cgaggccgct
     6061 ttagcaatac gtactttttc atcgtgattc tgcgcctgat gcagattcat cggcgtccag
     6121 cgcgggcgct gttgggtaat agcttctgcg ccattgcgca cctttttcag tttgccgctt
     6181 tcgccaagtt tattgatatc gcgtcgcgca gtggcaggcg aaattcccag acgctcaacg
     6241 actttctcaa cggtcacaaa gcccaattgt gcgagcattt ctaggaggat ttgatgtctt
     6301 tgtgcttcag tcatgagcta ttccgataaa atttgatttt tttagatgat atttgaaata
     6361 gccaggaaat actacgccgg atagcgcgaa cttctccacg ctatccggta taaacgacag
     6421 attacaggaa tgacttgaac ggcagatccg gttcaatagt gaagcaatca tcgaaaccgc
     6481 gcggatagtg gtactcgaag ttgtctttat ccagcggcca ggtaaatttg ccgccaacct
     6541 gccagataaa cggcttgaag ccatacttca gacgatcttt tttcatctcc cacagcacgc
     6601 ggatctcttg cggatcggcc tggaagtttg accagatgtc gtggtggaac gggataacca
     6661 ctttcgcatt cagggcttta cccatacgca gcatatcggc gctggtcatt ttgtcggtaa
     6721 taccgcgcgg gttctcacca tacgatccta acgccacatc gatctgatgt tcgttaccgt
     6781 gcttcgcata atagttagag tagtgggaat cgccactgtg atatagggaa ccgccagggg
     6841 ttttgaacag gtagtttact gcgcgatcgt ccatgccatc tggcagtacg ccagccgctt
     6901 tttgatcggc aggcagggtg atcagtgcag tacggtcgaa agcgtcaagc gcatgaattt
     6961 caatgtcttt cacttttaca acatcgcccg gtttgaccac gatgcaacgc tctttcggta
     7021 cgccccagcc aatccacaaa tcaacgcagg ttttcggtcc aatgaatggt acgtcatcag
     7081 cacagttctg catcacggca gcagcgacgt taacgtcgat atgatcgttg tgatcgtgag
     7141 tcgccagtac cgcgtcgatc tggcgaatcg caaacggatc aagaacaaac ggggtggtgc
     7201 gtaggtttgg ctgcagtttt ttcacgccag ccatgcgctg catctggtga ccctgtttca
     7261 ttaacgggtt accgtgactt tgtttgccag tgccgcacca gaaatcaacg caaacgttgg
     7321 tgccaccttc tgatttcaac cagatcccgg tgcagcctag ccaccacatc gcaaatgtgc
     7381 caggggcgac ctgctcttgt tcaatttctt cattcagcca gctaccccac tccgggaaag
     7441 tgctcaggat ccaggattct cgggtgatac ttttcacttt actcatcgcc atttaccttc
     7501 atgatagttc aattcgaatc aatatgtgat tggttttgat taatcctgac actatttttt
     7561 caggaaggca atgaccattt tttgactttt tgccagggaa gttgttgttg attttgagtg
     7621 tggaaagatt taatggaatg tgtagttaaa ttaactaaat gaatttaaat aaataattgt
     7681 ttcgttgtgt gaatcccgct ctatccgtgt ggaattattt gcgggtcgcg tcacatttaa
     7741 tcataaataa tcttgttgtg attacttttg aaaattagag tgagtgcaca acattccggg
     7801 tgtgcggaat acccggttac ctcttcttca ggagatcgtt atggagatcc tctacaacat
     7861 ctttaccgtg ttttttaacc aggtcatgac caatgccccg ttgttgctgg gtattgtgac
     7921 ctgtctgggc tacatcctac tgcgcaaaag tgtcagcgtt attattaaag gcacgattaa
     7981 aaccataatt agtttcatgt tgttgcaggc agggtccggc atcctcacca gcaccttcaa
     8041 accggtggtg gcaaaaatgt ccgaagtcta cggcattaac ggcgcaattt ccgataatta
     8101 cgcgtcgatg atggcaacca tcgaccgcat gggcgatgcc tatagctggg taggttacgc
     8161 ggtgttgtta gcgctggcgc tgaacatctg ttacgtgctg ctgcgtcgca ttaccggtat
     8221 tcgtaccatc atgctgactg gtcatatcat gttccagcag gcggggctga ttgccgttac
     8281 gttgtttatt ttcggctact ccatgtggac aaccattatc tgcacggcga ttctggtttc
     8341 gctctactgg ggcattacct ccaacatgat gtacaagccg actcaggaag tgacggacgg
     8401 ctgcggtttc tccatcggtc accagcaaca gtttgcatca tggattgcct ataaagttgc
     8461 gccgttcctc ggcaaaaaag aggaaagcgt tgaagacctc aaactgccag gctggctgaa
     8521 tattttccac gacaacatcg tctccacggc gattgtcatg accatcttct ttggtgccat
     8581 tctgctctcc ttcggtatcg acaccgtgca ggcgatggca ggcaaagtgc actggacggt
     8641 ctatatcctg caaaccggtt tttccttcgc ggtggcgatc ttcatcatca ctcagggtgt
     8701 gcgcatgttt gtggcggaac tctctgaagc gtttaacggt atctcccagc gcctgatccc
     8761 tggtgcggtt ttggcgattg actgtgcggc tatttatagc ttcgcgccga acgccgtggt
     8821 ctggggcttt atgtggggca ccatcggtca gctgattgcg gttggcatcc tggtcgcctg
     8881 cggttcctcg atcctgatta tcccaggctt tatcccgatg ttcttctcca acgccaccat
     8941 cggcgtgttc gctaaccact tcggcggctg gcgtgcggcg ctgaagattt gtctggtgat
     9001 ggggatgatc gaaatctttg gttgcgtctg ggcggtgaaa cttaccggta tgagtgcctg
     9061 gatggggatg gcggactggt cgattctggc accgccgatg atgcagggct tcttctccat
     9121 cggtatcgcc tttatggccg tcatcattgt aattgcactg gcttatatgt tcttcgctgg
     9181 ccgcgcactg cgcgcagaag aagatgcaga aaaacaactg gcagaacagt ctgcttaata
     9241 aggagttttg attatgaccg tacgtattct ggctgtgtgt ggcaacggac aaggcagttc
     9301 catgatcatg aagatgaaag tggaccagtt tttaacccaa tcaaacattg accacacggt
     9361 aaatagctgc gcggttggcg aatacaaaag cgagttgagc ggcgcggata tcatcatcgc
     9421 ttctacgcac attgcgggcg aaatcaccgt gaccggcaac aaatacgtgg ttggcgtgcg
     9481 caacatgctc tctcctgccg actttggccc gaaactgctg gaagtgatca aagcgcattt
     9541 cccgcaggat gtgaagtaag gacgcgccat gaaattacat gattcgctgg cggagaataa
     9601 atccatccgc ctgcaggctg aagctgagac atggcaggac gcagtaaaaa tcggcgttga
     9661 cctgctggtg gcggcggatg ttgtcgagcc gcgttactac caggcgattc tggatgcggt
     9721 tgaacagcac ggtccgtact ttgtgctcgc accaggcctg gcaatgccgc acggtcgccc
     9781 ggaagagggc gttaagaaaa ccggttttgc gctggtgacg ctgaaaaagc cgctggagtt
     9841 caaccacgaa gataacgacc cggtggatat cctcatcacc atggcggcgg tcgatgccaa
     9901 cacgcaccag gaagtgggca tcatgcagat cgtcaacctg tttgaagatg aagaaaattt
     9961 cgaccgttta cgtgcctgcc gtaccgagca ggaagtactg gatctcattg accgcaccaa
    10021 cgcggcagct taagaaggaa ttgaagatgt cattaccgat gttgcaagtc gcgctggaca
    10081 accagactat ggatagcgcc tacgaaacca ctcgcctgat tgccgaagaa gtcgacatta
    10141 tcgaagtggg taccattctg tgcgtgggcg aaggcgtgcg tgcggttcgc gatctgaaag
    10201 cgctctaccc gcacaaaatc gtgctggcag acgccaaaat tgccgatgcg ggcaaaattc
    10261 tttctcgtat gtgcttcgaa gccaacgctg actgggtgac cgtgatttgt tgtgcggata
    10321 tcaacaccgc caaaggtgcg ctggacgtag caaaagagtt taacggcgac gtgcagatcg
    10381 aactgaccgg ttactggacc tgggaacagg cgcaacagtg gcgcgacgca ggtattcagc
    10441 aggtggttta tcaccgcagc cgtgacgcgc aggccgctgg cgtggcgtgg ggtgaagccg
    10501 atatcactgc gatcaaacgt ctttccgata tgggcttcaa agtcaccgtc accggtggcc
    10561 tggcgctgga agatctgccg ctgttcaaag gcattccaat tcatgtgttt atcgccggtc
    10621 gcagcatccg tgatgccgct tctccggttg aagccgcgcg tcagttcaaa cgttccatcg
    10681 ccgaactgtg gggctaagga gcggatatgt tgtccaaaca aatcccgctt ggcatctatg
    10741 aaaaagcgct ccccgccggg gagtgctggc tggaacgcct gcgactggca aaaacgttgg
    10801 gcttcgattt tgtcgagatg tcggtagatg aaactgacga gtgcctgtcg cgcctcgact
    10861 ggagccgcga gcagcgtctg gcgctggtga atgcgattgt tgaaaccggc gtgcgtgtgc
    10921 cgtccatgtg cctttctgcc catcgtcgtt tcccgttggg cagcgaagat gacgcggtgc
    10981 gggcgcaggg gctggagatt atgcgtaaag ccatccagtt cgcccaggac gttggcattc
    11041 gcgtgatcca gctggcgggc tatgacgttt actatcagga agccaataac gaaacgcgtc
    11101 gtcgtttccg tgacggcctg aaagagagcg ttgagatggc gagccgcgcg caggtgacgc
    11161 tggcgatgga gatcatggat tatccgttga tgaactccat cagcaaggcg ctgggttacg
    11221 cgcactatct caacaacccg tggttccagc tctacccgga tatcggcaac ctgtcggcgt
    11281 gggacaacga tgtgcagatg gagttgcagg ccggaatcgg gcatatcgtc gcggtgcatg
    11341 tgaaagacac caaaccgggc gtcttcaaaa acgtgccatt tggcgaaggt gtggtggatt
    11401 tcgaacgttg cttcgaaacg ctcaaacaga gtggctattg cgggccgtac ctgattgaga
    11461 tgtggagcga aacggcggaa gacccggcgg cagaagtggt gaaagcgcgt gattgggtga
    11521 aagcgcgcat ggccaaagca ggcatggtgg aggcggcata atgcaaaagc taaaacagca
    11581 ggtgtttgaa gccaacatgg atctgccgcg ctatgggctg gtgaccttta cctggggcaa
    11641 cgtcagcgct atcgaccgcg aacgcgggct ggtggtgatc aagcccagcg gcgttgccta
    11701 cgaaaccatg aaagcagacg atatggtggt ggtcgatatg agcggcaagg tggtggaagg
    11761 ggagtatcgc ccatcttccg acactgcgac gcatctcgaa ctctaccgtc gttacccgtc
    11821 gcttggcggc attgtccata cccactccac tcatgccacc gcatgggcgc aggcggggct
    11881 ggcgatcccg gcgttaggca ccacgcacgc tgactacttc tttggtgata taccgtgtac
    11941 gcgtggatta agcgaagaag aggtgcaggg tgagtatgaa ctgaacactg gcaaagtgat
    12001 tatcgaaacg ctgggcaacg ccgagccgct gcatacgccg ggaattgtgg tgtatcagca
    12061 cggaccgttc gcctggggga aagatgccca cgacgcggtg cataacgcgg tagtgatgga
    12121 agaagtggcg aaaatggcgt ggattgcgcg cagtattaat ccacaactca atcatatcga
    12181 cagctacctg atgaataaac acttcatgcg taagcacggt cctaacgctt attacgggca
    12241 gaagtaaaac acgcgctgcg gaaatttcct tcctcgggag ataactggtc taattccgca
    12301 gccgtttttc aaaaaaagcc ccctgcgaag ggggcaaagc aaactatggc aatgtttcgt
    12361 tggttatacc tggtgctagc gataaatatc cgcgctggcg tgcatattgc cgttactccc
    12421 cggttcccgc atcagaatta cgtggtagta cgttgcgcct tgcgcatctg tttcttcatt
    12481 taatgcctga cgtgcttcgc tttcagtggc gaaattatga ttgatataaa tcacgcctaa
    12541 gctttgcaca tcgtccatat ttctggcctg gtggttatta atttcaatgg ctgcccatgt
    12601 atttgcactt agcaaaagca cagccagaag ggctaaaact cgactgaaca tagatacctc
    12661 ctcgacggct gactttgtgt gctctcctct gtgatgatct tctgatttaa ttttaatcaa
    12721 tgataaagaa gttgatggtg accatttctg atgcagttgt tcaaaaaaac actgtgacgg
    12781 aatgtgatga acttcaaatc agcggtttag aggttatttg cgaaagggga gatttatttc
    12841 tgctccgccc ttgagtttag cggggtatgt cagtactata acgcgtcatt tttcagccga
    12901 cctttaacac gttccttgcc tccccgggat tcggctgacc cagacaggag gctgaataat
    12961 ccgtaaggag caattcgatg cgtcattacg aaatcgtttt tatggtccat cctgaccaga
    13021 gcgaacaggt tccgggcatg atcgagcgct acactgttgc catcactggt gcagaaggca
    13081 agatccaccg tctggaagac tggggccgcc gtcagctggc ttacccgatc aacaaactgc
    13141 acaaagcaca ctacgttctg atgaacgttg aagctccgca ggaagtgatc gatgagctgg
    13201 aaactacctt ccgcttcaac gatgccgtta tccgcagcat ggttatgcgt accaagcacg
    13261 ctgttaccga agcatctccg atggttaaag cgaaagacga gcgccgtgag cgtcgcgatg
    13321 atttcgcaaa cgaaaccgct gatgatgctg aagctgggga ttctgaagag taatttctga
    13381 tgaccaaccg tctggtgttg tccggcaccg tgtgcaggac tccccttcga aaggtcagtc
    13441 catcaggaat tcctcactgc cagttcgtgc ttgagcatcg ttctgtgcag gaggaagccg
    13501 gctttcaccg gcaggcatgg tgtcaaatgc ccgttattgt tagcggacac gaaaaccagg
    13561 ccattactca cagtataacg gtcggcagtc gcataaccgt tcaggggttc atttcatgcc
    13621 acaaggcaaa gaacggactg agcaaaatgg ttttgcatgc cgagcagatt gaattgatag
    13681 attctggaga ctagccatat ggcacgttat ttccgtcgtc gcaagttctg ccgtttcacc
    13741 gcggaaggcg ttcaagagat cgactataaa gatatcgcta cgctgaaaaa ctacatcacc
    13801 gaaagcggta agattgtccc aagccgtatc accggtaccc gtgcaaaata ccagcgtcag
    13861 ctggctcgcg ctatcaaacg cgctcgctac ctgtccctgc tgccgtacac tgatcgccat
    13921 cagtaatcgg tcacggtcca ttaatacgac tttgagagga taaggtaatg caagttattc
    13981 tgcttgataa agtagcaaac ctgggtagcc tgggtgatca ggtaaacgtt aaagcgggct
    14041 atgctcgtaa cttcctggta ccgcagggta aagctgttcc agctaccaag aaaaacattg
    14101 aattcttcga agtacgtcgc gctgaactgg aagctaaact ggctgaagtt ctggcagctg
    14161 ctaatgctcg cgctgagaaa atcaatgcac tggaaactgt taccatcgcg tctaaagctg
    14221 gcgacgaagg taaactgttc ggttccatcg gtactcgcga catcgctgac gctgtaactg
    14281 cagctggcgt tgaagtggct aagagcgaag ttcgtctgcc gaacggcgtt ctgcgtacca
    14341 ctggcgaaca cgaagtgagc ttccaggttc acagcgaagt attcgcgaaa gtgatcgtaa
    14401 acgtagtagc tgaataattc gttattcaac gagacgtaaa aacgccgacc attggtcggc
    14461 gttttgcttt ctatttttcg tcaggcatta atttcgcaag tagatccatt caccattctc
    14521 ttgtgtccat aagtcaggat ggtttgctgg cgttagatgc atacgatcac tccttatgtc
    14581 tcggtcctgc ggcactcgct cgaaacctaa acccgagtag attgcggctg aatttgataa
    14641 ggaacgtaga ccaattttca aatcatgggg taaatcagaa tacgcgttta gcaatgcagc
    14701 aatgatgaat ccgagggcat tagaaacgcc tgtaacagtg cgaagttggt cgatataaag
    14761 atcgattgtt ggtagttcag atgaccagca attaggcatt tgggcaggaa gggttacaac
    14821 gtatcctatc gtttggccgg tatagtctgg agtactataa ccaggtccca aaacaacctc
    14881 gtgtattgtg tatttactgt tgggttcgaa ggattcgaca tcttgcaata aatatttcag
    14941 ttctgcggtt aacctcgcac ccgcgtaagc tttattgtat ttctgggcgt gaatattatc
    15001 taatgtagct tcatggttcc atccaaaaga attatcacca ttcattgtt
//
LOCUS       DADLSA010000095        14637 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.095, whole genome shotgun sequence.
ACCESSION   DADLSA010000095 DADLSA010000000
VERSION     DADLSA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.095"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(86..2062)
                     /gene="speA"
                     /locus_tag="JN691_002834"
     CDS             complement(86..2062)
                     /gene="speA"
                     /locus_tag="JN691_002834"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAY7244239.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2071..2202)
                     /gene="yqgB"
                     /locus_tag="JN691_002835"
     CDS             complement(2071..2202)
                     /gene="yqgB"
                     /locus_tag="JN691_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAY7244240.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            2338..2553
                     /locus_tag="JN691_002836"
     CDS             2338..2553
                     /locus_tag="JN691_002836"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244241.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2857..4011
                     /gene="metK"
                     /locus_tag="JN691_002837"
     CDS             2857..4011
                     /gene="metK"
                     /locus_tag="JN691_002837"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAY7244242.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4448..5842
                     /locus_tag="JN691_002838"
     CDS             4448..5842
                     /locus_tag="JN691_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY7244243.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5919..6416
                     /locus_tag="JN691_002839"
     CDS             5919..6416
                     /locus_tag="JN691_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAY7244244.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6511..7218
                     /gene="endA"
                     /locus_tag="JN691_002840"
     CDS             6511..7218
                     /gene="endA"
                     /locus_tag="JN691_002840"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAY7244245.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTHSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7298..8029
                     /gene="rsmE"
                     /locus_tag="JN691_002841"
     CDS             7298..8029
                     /gene="rsmE"
                     /locus_tag="JN691_002841"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAY7244246.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8042..8992
                     /gene="gshB"
                     /locus_tag="JN691_002842"
     CDS             8042..8992
                     /gene="gshB"
                     /locus_tag="JN691_002842"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAY7244247.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9029..9664
                     /locus_tag="JN691_002843"
     CDS             9029..9664
                     /locus_tag="JN691_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAY7244248.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKISPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9664..10080
                     /gene="ruvX"
                     /locus_tag="JN691_002844"
     CDS             9664..10080
                     /gene="ruvX"
                     /locus_tag="JN691_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAY7244249.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10319..11236)
                     /locus_tag="JN691_002845"
     CDS             complement(10319..11236)
                     /locus_tag="JN691_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAY7244250.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTG"
     gene            11254..11958
                     /gene="yggS"
                     /locus_tag="JN691_002846"
     CDS             11254..11958
                     /gene="yggS"
                     /locus_tag="JN691_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAY7244251.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11976..12542
                     /gene="yggT"
                     /locus_tag="JN691_002847"
     CDS             11976..12542
                     /gene="yggT"
                     /locus_tag="JN691_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAY7244252.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQSTGNMLLPGLWMAL"
     gene            12539..12829
                     /locus_tag="JN691_002848"
     CDS             12539..12829
                     /locus_tag="JN691_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAY7244253.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12837..13430
                     /locus_tag="JN691_002849"
     CDS             12837..13430
                     /locus_tag="JN691_002849"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAY7244254.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13423..14559
                     /gene="hemW"
                     /locus_tag="JN691_002850"
     CDS             13423..14559
                     /gene="hemW"
                     /locus_tag="JN691_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAY7244255.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         3531 a         3871 c         3753 g         3482 t
ORIGIN      
        1 gcagctagat tatttttaag aatatataaa aagaaagcgg gccgatttac gtaaataaat
       61 cgacccgcca gcggaataat cttaattatt catcttcaag ataagtataa ccgtacaaac
      121 ctgcctcgaa ctcttcaagg aactgttgtt gcagttcagc atcaagatcg gttttcttca
      181 cttgatcgcg gaactgggtt aacagcgttt tcggatcgag ctgtacatat tgcagcatgt
      241 ccgccacggt atcgccttcg tcagacagtt ctacttctac gctaccgtca gggaacacga
      301 acacgtcaac tgcttcggta tcaccgaaca ggttgtgcat attgccaagg atctcctgat
      361 atgcaccgac cataaagaaa ccgagcatcg gcggattctc tggatcgtac tccggcattg
      421 gcattgtcgt ggcaatacca tcaccatcaa tatagtggtc gatagcaccg tcagagtcac
      481 aggtaatatc cagcagcaca gcgcgacgtt ccggcacttg atccagccct tccagcggta
      541 gaaccgggaa caactggtcg atcccccatg cgtccggcat tgactggaac agcgagaaat
      601 tgacgtacat tttgtccgcc atacgttcct gcagttcgtc gattatcgga cgatgcgcac
      661 ggttttgcgg atccagttgc ttctgcactt catggcacat gctcagatag agttgttctg
      721 cccatgcacg ttcttgcaag ctaaaggtgc cggaagagta gccgatatga atgtcgtgca
      781 gatccatctg gctgtcgtgt aaccattcac gcaaagaacg gcgagttccc ggttcgtgca
      841 tttcctgcca ggtttcccac atgctttgca gcgcacgcgg cgcatcttct gcgggcgcgg
      901 tcggcaccgt atattcgtta cgttccacgc cgatgatatt cgacaccagc acggtatggt
      961 gcgcagtcac cgcacggccc gattcggtga ttaccgtcgg atgcggcaga ccattttctt
     1021 cacacgcatc gccaatcgcc cagatgatgt tgttggcgta ttcattgagg ccatagttta
     1081 cggagcagtc agactgcgaa cgggtgcctt cataatccac gcccagaccg ccgccgacgt
     1141 cgaagcactg aatattgacg cccagcttgt gcagttccac atagaaacgc gcggattcac
     1201 gaacgcctgt cgcgatatcg cgaatattcg ccatctgcga accgaggtgg aagtgcagta
     1261 gttgcaggct gtcgagacgc ccggcttcac gcagggtttc aaccagttgc agtacctgag
     1321 tcgcagccag gccgaacttc gatttttccc cgccggagga ctgccattta cccgagccct
     1381 gcgaagccag acgtgcacgc acgcccagac gaggaacgac attcagacgt tctgcttcat
     1441 ccagcacaat ggcgatttct gacatcttct caatgaccag atagaccttg tgccccatct
     1501 tctcgccaat taatgccagg cggatatatt cgcggtcttt ataaccgttg cagacgatga
     1561 cgctacgagt catgccagca tgtgccagca ctgccatcaa ctcggctttg gaaccggctt
     1621 ccagacccag cggttcaccc gaatgaatca gggactcgat cacgcggcgg tgctggttaa
     1681 ctttgatcgg ataaacaagg aagtaatcgc cgttatagcc gtaggattcc ctcgcacgtt
     1741 tgaacgcggc gttaatggaa cgcaaacggt gctgcaggat ctgtgggaaa cagaacagtg
     1801 caggcagacg ctgaccctgt gcttcacgag ttttcactaa ctgcgcgaga tcgacgcgag
     1861 cttccgggac gtccgggtcc gggcacacgc tgatgtggcc cagctcgtta acgtcatagt
     1921 agttattgcc ccaccaggca atattgtaag tacgcagcat cttgctggct tcctgggagc
     1981 tcattgcaac ctcctgcatg gagcgtagta caccgtgttc gcccgctgac gaaggcaaac
     2041 ccatagacat gtcgtcagac atagcgaacc tcaaattatt ttattaagtg taaaacagtt
     2101 aacgactatc gcagcctgga actgcgataa caacccataa gcacatggat tttccagcag
     2161 tgtatgctga cgctccgact gcgcaaccgg tttctttttc ataacattat taagcacata
     2221 accgaacgta agtgtgacag ttcggcgaaa ccacgagaaa actcttgttt ttacaagagc
     2281 gcccttgttc agtcctcagt aactgtaacc agctcttgaa tcctgagaag cgccgagatg
     2341 ggtataacat cggcaggtat gcaaagcaga gatgcagagt gcggggaacg aatcttcacc
     2401 agaacggtga gacaggttaa gcagcagaca acggttcatt atttcgtatc acctccacgg
     2461 ccgcctgtta agacgaaccc acaagccaaa actctgattt caacccggct ggaagtggca
     2521 acacgaaaga aacgtcgtgt gctttttatt taagccgcgc gccgcgtttt ataccccaca
     2581 atggcagaaa attgcaaaag ataaatacgc agaatgccgg cattgtcagg aaaaatttcc
     2641 agccacgttt ttaacagaat gagacacgat tcaaaaaaaa gtggaaatcg ggtgaagaat
     2701 tgacctaaaa tagccatcca gatgttaatc catccatacc gattaacact cagactgcca
     2761 gtgtttttaa cctgcagagt cgtggtagga tccgctacca cagaaaatcc acacaacagt
     2821 ttgagctaac caaattctct ttaggtgata ttaaatatgg caaaacacct ttttacgtcc
     2881 gagtccgtct ctgaagggca tcctgacaaa attgctgacc aaatctctga tgccgtttta
     2941 gacgcgatcc tcgaacagga tccgaaagca cgcgttgctt gcgaaaccta cgtaaaaacc
     3001 ggcatggttt tagttggcgg cgaaatcacc accagcgcct gggtagacat cgaagagatc
     3061 acccgtaaca ccgttcgcga aattggctat gtgcattccg acatgggctt tgacgctaac
     3121 tcctgtgcgg ttctgagcgc tatcggcaaa cagtctcctg acatcaacca gggcgttgac
     3181 cgtgccgatc cgctggaaca gggcgcgggt gaccagggtc tgatgtttgg ctacgcaact
     3241 aatgaaaccg acgtgctgat gccagcacct atcacctatg cacaccgtct ggtacagcgt
     3301 caggctgaag tgcgtaaaaa cggcactctg ccgtggctgc gcccggacgc gaaaagccag
     3361 gtgactttcc agtatgacga cggcaaaatc gttggtattg atgctgtcgt gctttccact
     3421 cagcactctg aagagatcga ccagaaatcg ctgcaagaag cggtaatgga agagatcatc
     3481 aagccaattc tgcccgctga atggctgact tctgccacca aattcttcat caacccgacc
     3541 ggtcgtttcg ttatcggtgg cccaatgggt gactgcggtc tgactggtcg taaaattatc
     3601 gttgatacct acggcggcat ggcgcgtcac ggtggcggtg cattctctgg taaagatcca
     3661 tcaaaagtgg accgttccgc agcctacgca gcacgttatg tcgcgaaaaa catcgttgct
     3721 gctggcctgg ccgatcgttg tgaaattcag gtttcctacg caatcggcgt ggctgaaccg
     3781 acctccatca tggtagaaac tttcggtact gagaaagtgc cttctgaaca actgactctg
     3841 ctggtacgtg agttcttcga cctgcgccca tacggtctga ttcagatgct ggatctgctg
     3901 cacccgatct acaaagaaac cgcagcatac ggtcactttg gtcgtgaaca tttcccgtgg
     3961 gaaaaaaccg acaaagcgca gctgctgcgc gatgctgccg gtctgaagta atctttcttc
     4021 acctgcgttc aaaggccagc ctcgcgctgg cctttttctt ttacctcacc gcggtggata
     4081 ggcgttcacg ccgcatccgg caaaaaaact gcccgcacaa taacatcatt cttcctgatc
     4141 acgtttcacg gcaaattacc atcacaactg aaaccgatta caccaaccac accagcaaaa
     4201 tatttgtaat attttcatat tgttattcag ctttcacagt tgttacattt cttttcagta
     4261 aagtcttaat tgcagatgtc agcgtttaat ctatgatgat ataactcaat tattttcatg
     4321 cacttaaatc ataactaaga taaatgttag tgtaagcgat tacactaatg tgatttgctt
     4381 cacatctttt tacgtcgtac tcacctatct taattcacaa taaaaaataa ccatattgga
     4441 gggcatcatg cctgacgcta aaaaacaggg gcggtcaaac aaggcaatga cgtttttcgt
     4501 ctgcttcctt gccgctctgg cgggattact ctttggcctg gatatcggtg taattgctgg
     4561 cgcactgccg tttattgcag atgaattcca gataacttcg cacacgcaag aatgggtcgt
     4621 aagctccatg atgttcggtg cggcagtcgg tgcggtgggc agcggctggc tctcctttaa
     4681 actcgggcgc aaaaagagcc tgatgatcgg cgcaatcctg tttgttgccg gttcactgtt
     4741 ctctgcggct gcgccaaacg ttgaagtact gattctttcg cgcgttctgc tggggctggc
     4801 ggtgggtgtg gcctcttata ccgcaccact gtacctgtca gaaattgcgc cggaaaaaat
     4861 tcgcggcagt atgatctcga tgtatcagtt gatgatcact atcgggatcc tcggtgctta
     4921 tctttctgat accgccttca gctacaccgg tgcatggcgc tggatgctgg gtgtgattat
     4981 catcccggca attttgctgc tgattggtgt cttcttcctg ccagacagcc cacgttggtt
     5041 tgccgccaaa cgccgttttg ttgatgccga acgcgtgctg ctacgcctgc gtgacaccag
     5101 cgcggaagcg aaacgcgaac tggatgaaat ccgtgaaagt ttgcaggtta aacagagtgg
     5161 ctgggcgctg tttaaagaga atagcaactt ccgccgtgcg gtgttccttg gcgtactgtt
     5221 acaggtaatg cagcaattca ccgggatgaa cgtcatcatg tattacgcgc cgaaaatctt
     5281 cgaactggcg ggttatacca acaccaccga gcaaatgtgg gggacagtga ttgtcggcct
     5341 gaccaacgta cttgccacct ttatcgcaat cggccttgtt gaccgctggg gacgtaaacc
     5401 aacgctaacg ctgggcttcc tggtgatggc tgctggcatg ggcgtactcg gtacaatgat
     5461 gcatatcggt atccactctc cgtcggcgca gtatttcgcc atcgccatgc tgctgatgtt
     5521 tattgtcggt tttgccatga gtgccggtcc gctgatttgg gtactgtgct ccgaaattca
     5581 gccgctgaaa ggccgcgatt ttggcatcac ctgctccacc gccaccaact ggattgccaa
     5641 catgatcgtt ggcgcaacgt tcctgaccat gctcaacacg ctgggtaacg ccaacacctt
     5701 ctgggtgtat gcggctctga acgtactgtt tatcctgctg acattatggc tggtaccgga
     5761 aaccaaacac gtttcgctgg aacatattga acgtaatctg atgaaaggtc gtaaactgcg
     5821 cgaaatcggc gctcacgatt aatctcctca ggcttcctcc cgtcgcggag gaagccgcct
     5881 cttgcagtca tcttttcttc gctctatcct ctgccgctat gaaaacatcc cgtctcccta
     5941 tcgccatcca acaggccgtt atgcgccgcc tgcgggaaaa actcgcccag gccaacctga
     6001 agctagggcg taactacccg gagccaaaac tctcttacac ccagcgcgga acctccgccg
     6061 gaacagcctg gctggaaagc tatgaaatcc gcctcaatcc cgttttgctg ttggaaaaca
     6121 gtgaagcttt tattgaagaa gtggtaccgc acgaactggc acatttgctg gtatggaaac
     6181 atttcggccg cgtagcgcca catggcaaag agtggaagtg gatgatggaa agcgtgctgg
     6241 gcgttcccgc ccgtcgtacg catcagttcg aactgcaatc cgtgcgtcgc aacaccttcc
     6301 cctaccgctg taagtgccag gaacatcagc ttaccgtacg ccgccataat cgcgtagttc
     6361 gtggcgaggc catctatcgc tgtgttcact gcggtgaaca gctggttgcg aaataaccac
     6421 ctgaactatc aggaactttc ctgatctggc tgattgcata ccaaaacaac tttcgctacg
     6481 ttgctggctc gttttaacac ggagtaagtg atgtaccgtt atttgtctat tgctgcggtg
     6541 gtactgagcg cagcattttc cggcccgacg ttggccgaag gtatcaatag tttttctcag
     6601 gcgaaagccg cggcggtaaa agtccacgct gatgcgcccg gtacgtttta ttgcggatgt
     6661 aaaattaact ggcagggcaa aaaaggcgtt gttgatctgc aatcgtgcgg ctatcaggtg
     6721 cgcaaaaatg aaaaccgcgc cagccgcgta gagtgggaac acgtcgttcc cgcctggcaa
     6781 ttcggtcacc agcgccagtg ctggcaggac ggtggacgta aaaactgcgc taaagatccg
     6841 gtctatcgca agatggaaag cgatatgcat aacctgcagc cgtcagtcgg cgaggtgaat
     6901 ggcgatcgcg gcaattttat gtacagccag tggaatggcg gcgaaggcca gtacggtcaa
     6961 tgcgccatga aggtcgattt caaagaaaaa gctgccgaac caccagcgcg tgcacgcggt
     7021 gccattgcgc gcacctactt ctatatgcgc gaccaataca acctgacaca ctctcgccag
     7081 caaacgcagc tgttcaacgc atggaacaag atgtatccgg ttaccgactg ggagtgcgag
     7141 cgcgatgaac gcatcgcgaa ggtgcagggc aatcataacc cgtatgtgca acgcgcttgc
     7201 caggcgcgaa agagctaacc tacactagcg ggattctttt tgttaacccc taccccacgc
     7261 gtacaaccgc gtggggagac gacgcggatt tttaactatg cgtatccccc gcatttatca
     7321 tcctgaacca ctgaccagcc attctcacat cgcgctttgc gaagatgccg ccaaccatat
     7381 cgggcgcgta ctgcgcatgg ggccggggca ggcgttgcaa ttgtttgacg gtagcaacca
     7441 ggtctttgac gccgaaatta ccagcgccag caaaaaaagc gtggaagtga aggtgctgga
     7501 aggccagatc gacgatcgcg aatctccgct gcatattcac ctcggtcagg tgatgtcgcg
     7561 tggtgaaaaa atggaattta ctatccagaa atcgatcgaa ctcggtgtaa gcctcattac
     7621 gccacttttt tctgagcgct gcggcgttaa actggatagt gaacgtctga acaagaagct
     7681 tcagcagtgg cagaagattg caattgctgc ctgtgagcag tgtggtcgta accgggtgcc
     7741 ggaaatccgt ccagcgatgg atctggaagc ttggtgtgca gagcaggatg aaggactgaa
     7801 actgaatctt cacccgcgcg ccagtaacag catcaatacg ttgcctttac cggttgaacg
     7861 cgtccgcctg ctgattggcc cggaaggcgg tttatcggca gatgaaattg ccatgactgc
     7921 ccgctatcaa tttactgata tcctgttggg acctcgcgtt ttgcgtacag agacaactgc
     7981 gctcaccgcc attaccgcgc tacaagtacg atttggcgat ttgggctaac ggagaagaat
     8041 aatgatcaag ctcggcatcg tgatggaccc catcgcaaac atcaacatca agaaagattc
     8101 cagtttcgct atgttgctgg aagcacagcg tcgtggttac gaacttcact atatggagat
     8161 gggcgatctg tatctgatca atggcgaagc ccgcgcccat acccgcacgc tgaacgtgaa
     8221 gcagaactac gaagagtggt tttcgttcgt cggtgaacag gatctgccgc tggccgatct
     8281 cgatgtgatc ctgatgcgta aagacccgcc gtttgatacc gagtttatct acgcgaccta
     8341 tattctggaa cgtgccgaag agaaagggac gctgatcgtt aacaagccgc agagcctgcg
     8401 cgactgtaac gagaaactgt ttaccgcctg gttctctgac ttaacgccag aaacgctggt
     8461 tacgcgcaat aaagcgcagc taaaagcgtt ctgggagaaa cacagcgaca tcattcttaa
     8521 gccgctggac ggtatgggcg gcgcgtcgat tttccgcgtg aaagaaggcg atccaaacct
     8581 cggcgtgatt gccgaaaccc tgactgagca tggcactcgc tactgcatgg cgcaaaatta
     8641 cctgccagcc attaaagatg gcgacaaacg cgtgctggtg gtggatggcg agccggtacc
     8701 gtactgcctg gcgcgtattc cgcagggggg cgaaacccgt ggcaatctgg ctgccggtgg
     8761 tcgcggtgaa cctcgtccgc tgacggaaag tgactggaaa atcgcccgtc agatcgggcc
     8821 gacgctgaaa gaaaaagggc tgatttttgt tggtctggat atcatcggcg accgtctgac
     8881 tgaaattaac gtcaccagcc caacctgtat tcgtgagatt gaagcagagt ttccggtgtc
     8941 gatcaccgga atgttaatgg atgccatcga agcacgttta cagcagcagt aacccacctt
     9001 agcgagaagg atctcgttga gactctgagt gacagcgccc ttctttccac gcatactggg
     9061 cgctgttgct tttttgaacc aggaaacaga acctctgaca atgaatttac agcatcactt
     9121 tcttattgcc atgcctgctc tccaggatcc gattttccgt cgttccgtgg tctacatttg
     9181 cgaacataat accaatggtg caatggggat catcgtcaac aagccgctgg aaaatctcaa
     9241 aattgaaggg attctggaaa agctgaagat ctcgccggag ccgcgtgatg aatcaatccg
     9301 tctggataaa ccggttatgc tcggcggccc actggctgaa gatcgcgggt ttattttgca
     9361 tactccgccc tccaattttg cttccagcat tcgcatttca gacaacacgg tgatgaccac
     9421 ttcccgcgat gtgctggaaa cgctcggcac cgataaacaa ccgtctgacg tattggtggc
     9481 tctgggctat gcctcctggg aaaaaggtca actggaacaa gaaattctcg ataacgcgtg
     9541 gctaacggcc ccggcggatc tgaatattct gttcaaaacg ccgattgccg accgctggcg
     9601 cgaggcggca aaactgattg gtgtggatat tctcaccatg cctggtgtgg caggacacgc
     9661 ctgatgagtg gaaccttact cgccttcgac ttcggcacca aaagcattgg cgtagcggtc
     9721 ggccaacgca ttaccggcac cgctcgccct ttgcctgcaa ttaaagcgca ggacggtacg
     9781 ccggactgga acctcatcga acgtttactg aaagagtggc agcctgacga aatcatcgtc
     9841 ggtttgccgc tgaatatgga cggcaccgaa cagccgttaa ccgccagagc gcgcaaattt
     9901 gccaaccgta ttcatggtcg tttcggtgtt gaagtaaagc tccatgacga gcgtcttagc
     9961 actgtggaag cccgttccgg tctttttgaa cagggcggct atcgagcgct caacaaaggc
    10021 aaagttgact ctgcctctgc ggttattatt ctcgaaagct atttcgagca gggatattaa
    10081 ggcgatctaa aagcctggcg gatagtcgtt accgccatct tgccggatgc ggcgtgaacg
    10141 ccttatccag cacacatctg gcagcggctg taggtctgat aagacgcgtt agcgtcgcat
    10201 cagacattta ttgcctttat aggccctata aacttgcgca tcgggcatgg caacgtcaca
    10261 aacgcccttc ccccacccgc tgctgataac tctgctgaaa cgttaacatc cccacctgct
    10321 acccggtttg aataacatgc ggtaactggt gggttttccc ttcgcgaatc aaattcccca
    10381 ccgcgggagt gttaatcagt aattcaaaca gcgccacgcg tccttcctgt ttatccactt
    10441 ccagcttttg tgacagcact gcccgtaaac tacccgcaag ttgattacgt acgggatctt
    10501 tttcctgcgc cggaaatgaa tccaccagtc gctcaactgc ctgcgcggca ccacgtgtat
    10561 gtaaggttgc cagcaccaga tgcccggttt ctgccgccgt cagcgccaga cggattgtct
    10621 cactgtcacg tagctctccg agcaaaatca catcaggatc ttcccgcaat gcagcccgca
    10681 accccgatgc gaacgtcata cagtgcaaac caatttcccg ctgctggatc aaacatcgct
    10741 ggctggcata gagatattcc acaggatctt ccagcgtcag aatatgcgca tcggcatgtt
    10801 gattaagata gccaaccatc gccgccagcg tggtagattt gccactcccc gtagcccccg
    10861 tcaccagaat caggccattc tcgctcttga gtaattccgg caataccggt ggcgcaccaa
    10921 gctgttcgag ctgtggacag tgcgaaggta acaaccgtaa tgccagagaa ataccttgcc
    10981 gttgcgcgaa cgcactgcca cgcaaccgct ggttttccgc cagcgacacg gcaaaatcca
    11041 gctggccatt ttccagcaat attgcccgct gatcgtcatc cagccactcc cgcagtagct
    11101 cttcgacgtc cggcgcatca aacggcgcag cttccattct gccacgaatg cgccatcgtg
    11161 cgggccaggc gctgcacagg tgtagatccg agacgttatg ctttacacta agggccacaa
    11221 tttcttccat attcatacta agatcctcgg aaaatgaacg atattgcgca taacctggca
    11281 caggtccggg acaaaatctc agcggctgca acgcgttgcg gccgttctcc agaagaaatt
    11341 acgctgcttg cagtcagtaa aacaaaacct gcgagcgcca tcgcagaagc cattgatgcc
    11401 gggcagcgtc aatttggtga aaactacgtt caggaagggg tagataaaat tcgccacttt
    11461 caggaactgg gcgtaacagg attagaatgg cattttattg gcccgttgca gtctaataaa
    11521 agccgcctag tggcagagca tttcgactgg tgtcatacca tcgaccgttt gcgcattgct
    11581 acccgcctca acgatcagcg ccctgcagaa cttccccctc ttaacgtact gattcaaatt
    11641 aatattagtg atgaaaacag taagtccggg attcaactgg cagaactgga cgagctggca
    11701 gcggcggtcg ctgaactacc gcgtttacgt ctgcgcgggc tgatggcaat ccctgcgcct
    11761 gagtcagaat atgtaaggca gtttgaagtt gcacgccaaa tggctgtagc atttgccgga
    11821 ctgaaaacgc gctacccgca tatcgacacg ctctctctgg gaatgtcgga cgatatggaa
    11881 gccgccattg cggcaggtag cacgatggtt cgtatcggca ctgcaatttt tggtgcgcgt
    11941 gattactcta aaaaataagg aattaaagga acgccatgaa tacgttgact ttcctgcttt
    12001 caacggtcat tgagctgtat accatggtgc tgttattacg catctggatg cagtgggctc
    12061 attgtgattt ttacaacccc ttctcacagt ttgtagtgaa ggtagcgcag ccaattatcg
    12121 ggccactgcg ccgcgttatt ccggcaatgg gtcccattga cagcgcctcg ctgctggttg
    12181 cctatattct cagttttatc aaagccattg tgctgtttga agtggtgacc ttcctgccaa
    12241 tcatctggat tgccggttta ctgattctgc tgaaaaccat cggcttgctg attttctggg
    12301 tcttgctggt gatggcgatt atgagctggg taagccaggg tcgtagcccg attgaatacg
    12361 tgctgattca gctggccgat ccgctgctgc gcccgattcg ccgcttgcta ccggcaatgg
    12421 gtgggattga tttctcgccg atgatcctcg ttctgctgct gtatgtcatc aatatgggtg
    12481 tcgcagaagt attacagtca accggaaaca tgctgctgcc ggggctgtgg atggcgttat
    12541 gagtgccgta acagttaatg atgacggtct ggttttacgg ctctatattc agccgaaagc
    12601 cagccgtgat tctattgtcg gtttacatgg cgacgaagtt aaagtcgcca ttaccgcgcc
    12661 gccggttgac ggccaggcca acagtcatct ggtgaagttt ctcggtaagc aattccgggt
    12721 tgccaaaagc caggtggtga ttgaaaaagg cgaacttggc cgccacaaac aaattaaaat
    12781 cattaatccg caacaaatcc cgccagaaat cgcggcgtta attaattagg tatcctatgc
    12841 aaaaagttgt cctcgcaacc ggcaatgccg gtaaagtgcg tgagctggcg tcgctgctta
    12901 gcgactttgg tcttgatatc gtggcccaaa cagacctcgg cgttgattcc gctgaagaaa
    12961 ccggcctaac ctttatcgaa aacgcgattc tgaaagcgcg ccatgcggca aaagtgaccg
    13021 gtttaccggc aattgccgac gactctggtc tggcggtaga tgcgctgggt ggcgcgccgg
    13081 gtatttactc cgcgcgttat tccggtgaag acgcgaccga tcaaaagaat ctgcaaaaac
    13141 tgttggaaac actgaaagac gtaccggacg accaacgtca ggcgcgtttc cactgcgtgc
    13201 tggtatatct gcgtcacgcg gaagatccga ctccgctggt atgccacggc agctggccgg
    13261 gcgtgattac tcgcgaacca gcgggcactg gtggctttgg ttatgatcca atcttcttcg
    13321 taccttccga agggaaaacc gccgccgaac tgacccgcga agaaaagagc gccatttccc
    13381 accgtggtca ggcgttgaaa ctgctgctgg acgctttacg taatggttaa attaccgccg
    13441 ctgagtctct acattcacat cccgtggtgc gtgcagaaat gcccgtactg cgatttcaac
    13501 tctcacgcgt tgaaaggaga agtgccgcac gacgattatg ttcagcatct gctttgcgat
    13561 ctggacaatg atgtggctta cgctcagggc cgtgaagtaa agacaatctt tattggcggt
    13621 ggtacgccga gcctgctttc cggcccggcg atgcaaacgc tgctggacgg cgtgcgtgcg
    13681 cgtttgccgc tggcagcgga tgcagaaatt actatggaag cgaacccagg cacggtagaa
    13741 gcggatcgct ttgtcgatta tcagcgtgct ggtgtaaacc gcatctctat tggtgtgcag
    13801 agttttagcg aagaaaagct gaaacgactt gggcgtattc atggcccgca agaagcgaaa
    13861 cgagcggcaa agctggcgag cggtttaggg ttacgtagct ttaaccttga tttgatgcat
    13921 ggactaccgg atcaatcact ggaagaggcg cttggcgatc tacgccaggc cattgaactg
    13981 aatccgccgc atctttcctg gtatcaactg accatcgaac ctaatacgct gtttggctcg
    14041 cgaccgccgg tgctgccgga cgatgacgcg ctgtgggata ttttcgaaca ggggcatcag
    14101 ttattaaccg cagcgggtta tcagcaatat gaaacttccg cttacgccaa acccggttat
    14161 cagtgccagc acaatctcaa ctactggcgc tttggtgact acatcggtat tggctgcggc
    14221 gcgcacggca aagtgacctt cccggatggg cgcattctgc gtaccactaa aacgcgtcat
    14281 cctcgtggtt ttatgcaagg aaggtatctg gaaagccagc gtgatgtcga agccgcagat
    14341 aagccgtttg agttctttat gaatcgcttc cgtctgctgg aggccgcgcc gcgcgtggag
    14401 tttattgcgt ataccgggct ttgcgaagat gtgattcgcc cacagttaga cgaggcgatt
    14461 gcccagggtt atctcaccga atgtgcggat tactggcaga taacggaaca tggtaaatta
    14521 ttcttaaact cacttcttga attattcctc gctgagtaat cattaatgcg cctgcgggcg
    14581 cattaatcac ttcttttatc cccccgttaa ttaacctcgg cagaaatata tttctta
//
LOCUS       DADLSA010000096        14605 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.096, whole genome shotgun sequence.
ACCESSION   DADLSA010000096 DADLSA010000000
VERSION     DADLSA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.096"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(175..1368)
                     /locus_tag="JN691_002851"
     CDS             complement(175..1368)
                     /locus_tag="JN691_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7244256.1"
                     /translation="MSPGIEDTPQKPQSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1504..3228
                     /gene="iucA"
                     /locus_tag="JN691_002852"
     CDS             1504..3228
                     /gene="iucA"
                     /locus_tag="JN691_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAY7244257.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3229..4176
                     /gene="iucB"
                     /locus_tag="JN691_002853"
     CDS             3229..4176
                     /gene="iucB"
                     /locus_tag="JN691_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAY7244258.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4176..5918
                     /gene="iucC"
                     /locus_tag="JN691_002854"
     CDS             4176..5918
                     /gene="iucC"
                     /locus_tag="JN691_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAY7244259.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            5915..7252
                     /gene="iucD"
                     /locus_tag="JN691_002855"
     CDS             5915..7252
                     /gene="iucD"
                     /locus_tag="JN691_002855"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAY7244260.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7258..9453
                     /gene="iutA"
                     /locus_tag="JN691_002856"
     CDS             7258..9453
                     /gene="iutA"
                     /locus_tag="JN691_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAY7244261.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            10413..14300
                     /gene="sat"
                     /locus_tag="JN691_002857"
     CDS             10413..14300
                     /gene="sat"
                     /locus_tag="JN691_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HAY7244262.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSTANHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         3896 a         3499 c         3640 g         3570 t
ORIGIN      
        1 gatcataggt tgttgcacga aagaatcatg gagattacac cgcagcaact ggttgaattc
       61 actatacctg atgggcgggg gcagcaagcc agaacactgc ctgacatgct ttcatcagac
      121 gcaggtcctc tctcatatcc tctgatttac tccagtaagg tgatgatcat atctttattt
      181 gatagtacgg taatgccaga taaatacaga cacaatggta aataatatag ttgcaaataa
      241 tgctgccata atcagcgcga ttgcgacacc tgttttcgat accagcggca ccagcaaagc
      301 gcacagtcca tagccaagtg tgtgactggt agcgagccag cccgcgcctg cgccatcagc
      361 cagcttatca ttcagtagaa gttgataagc gggtgttgcc agagctgctc caaatgacag
      421 cactgcacag cccgggtaaa acaaccatag ctgttcggag agcatgatag ccagaccacc
      481 actcatcaac actccggcac tcaggagcag ggccaccgga gtcaggcgct gcggacggag
      541 taccccgaac tgcgcgataa gcgcagctac tgcggacaga cccaacagcc aggccacctg
      601 ttggctaatg gcggtggtat ccgtagcgaa ctggcgagta agggcaggcg aaagtccaag
      661 ctgcatcatg ctcaccgccg cagccagtaa tattgcgcaa agcagataag gcagacaatc
      721 tcgcttcaga ctgacgctct tgcactccgg tgtgggctgt ggtggtgtgc cgggcaaccg
      781 cagcagcatc agcagcgcca gcactggggc tgccatcagc agccccagtg gcgccagagg
      841 atgaatggcc agcattgctg ccgcgcacag cggcccgaat agcctgccgc aactcaggcc
      901 ggagctgatg gttgccagag cggccatgcg attcccttct ccagctctct gcaatgccca
      961 gacctgacag gctggcacca ttgccgacac ggtcagcccg taggcgatcc gggcgatgat
     1021 taatattccc agtcccaccg ctgttgttat ggcgctggta gccatcagca cgcttcccag
     1081 ccctagcagg gtaaagctta tgccatatcc tcccagagcc cacaacacca caggtttact
     1141 gccagtacgg gaaatttgct ttccccacca cggggacgat ggcagaaaca gcattgagcc
     1201 aagcatcagc agggcagccc atacagacag actcagattt gtctgtatga ccagaacagg
     1261 gagcacaacc agcagaccgt tctgtccgat accgagaagc ccggcactga acgcaagtgg
     1321 ccagcaggac tgtggttttt ggggcgtatc ttcgatccct ggtgacatta taatgtttca
     1381 actccatgta ttaattatgt ttatttgtaa aattaattta tctgacaata acattcattg
     1441 ttgataatga gaatcattat tgacataatt gttattattt tactgtgtgg gagctgtttg
     1501 actatgaccc tgccctctga aaaaccagcc acagatgtgg ctgcgcagtg cttcctgaat
     1561 gcactgattc gtgaaaccac agactggaaa ctgacagaat acccgccaga cgaattgctc
     1621 atcccgctgg atgagcagaa atcgctccat ttcagagtgg cttatttctc cccaacccag
     1681 catcaccgct ttgcatttcc tgcacgtctg gtcacggcat caggcagtta tcctgtcgac
     1741 tttaccactc tctcccggct tattattgat aagctacgcc atcaactgtt tctgcccgtt
     1801 cccctctgcg aaactttcca ccagcgcgtg ctggaaagcc atgtccatac gcaacaggca
     1861 attgatgccc gtcatgactg ggccgctctg cgtgaaaaag cgttgaattt tggcgaggct
     1921 gagcaggcgc tgctgacagg acacgctttc caccctgcgc ctaagtctca tgaaccgttt
     1981 aaccggcggg aggctgaacg ctacctgcct gatatggcac cccacttccc actgcggtgg
     2041 ttttcggtgg ataaaacgca aatcgccggt gaaagtttgc atctcaacct tcaacagcgg
     2101 ctgacgcgat ttgccgcaga gaatgcgcct cagttactca acgaattaag tgacaaccaa
     2161 tggctgttcc cgttgcaccc gtggcagggg gaatatcttt tgcagcaggg gtggtgccag
     2221 gcacttgttg ctaaagggct gattaaagac ttaggtgagg ccggcacgtc gtggctgccg
     2281 accacctctt cccgttccct ctactgtgcc accagccgcg atatgatcaa gttctccctg
     2341 agcgtacggc tgaccaactc catccgtact ctgtccgtga aagaagtgaa gcgtgggatg
     2401 cgcctggcac gcctggctca aaccgacggc tggcagatgc tacaggtccg cttcccgact
     2461 ttccgggtaa tgcaggagga tggctgggcc gggctgctcg atcttaacgg caacatcatg
     2521 caggaaagtc tgtttgccct gcgtgaaaat ctgctggtgg atcagccgaa aagccagact
     2581 aacgtactgg tctccctgac tcaggccgca cctgatggcg gtgattcgct gctggtttcg
     2641 gcggtaaaac gcctgagcga tcgcctcggt atcactgtgc aacaggccgc ccatgcatgg
     2701 gtcgatgcat actgtcagca agtgctaaag ccgctgttta cggctgaagc ggattacggc
     2761 ctggtgctgc tggcgcatca gcaaaatatt cttgtccaga tgcttgggga tctgccggtc
     2821 ggatttattt accgtgactg tcagggcagc gcttttatgc ctcacgcgac agactggctc
     2881 gattctattg gcgaggcgca ggcggaaaat attttcaccc atgagcagtt gctgcgctat
     2941 ttcccttatt acctgctggt taactccact tttgctgtga ccgctgcgct gggggctgcc
     3001 gggctggaca gcgaatcgaa tctgatggct cgtgtacgag catcgctggc tgaagtgcgt
     3061 gatcaggtga ctcataaaac ctgtctcaac tacgtgctgg aaagtccgta ctggaacgta
     3121 aaaggtaact ttttctgtta tctgaacgat cataacgaga ataccattgt tgacccttcg
     3181 gtgatctatt tcgatttcgc taacccgctg caggctcagg aggtctgaat gtctgaggca
     3241 aacattattc acagcagata tggactgcgc tgtgaaaaac tcgacaagcc cctgaatctt
     3301 ggctggggac tggacaatag cgcggtgttg cactgtcccg gggagctgcc gacagggtgg
     3361 ctgtgcgatg cgctggatca gatatttatc gccgcaccac aactgtcagc agtggctctc
     3421 ccctgggccg aatggcgtga ggagccacag gcgctgacgc tttttgggca ggtaaaaagc
     3481 gacattatcc atcgtactgc cttctggcag ttaccgttat ggttgagttc accggcaaac
     3541 cgggcctccg gtgaaatggt ttttgatgca gagcgtgaga tttatttccc gcagcgccca
     3601 ccccgtccac agggtgaggt ttaccgccgt tatgatccac gtattcgcag gatgctgagt
     3661 ttccgcattg ccgatcccgt ttctgatgca gaacgtttca cccgctggat gaatgatccg
     3721 cgcgttgagt atttctggga gcaaagtggc tcactggagg tacagaccgc ctatctggag
     3781 cgccagctca ccggtaaaca tgcattcccg ctgattgggt gcttcgacga tcggccattc
     3841 agctatttcg aaatctactg ggcggcggaa gaccgcattg gtcgtcacta ttcgtggcag
     3901 ccctttgacc gtggcctgca tctgctggtt ggtgaacagc aatggcgcgg ggcccactat
     3961 gtgcaaagct ggctgcgcgg gctgacacat tacctgctgc tggatgagcc ccgtacgcag
     4021 cgcacagtac tggagccacg caccgataac cagcgcctgt tccgccatct tgagcctgcg
     4081 ggataccgga caattaaaga gttcgacttc ccacataagc gctcgcgcat ggtgatggcg
     4141 gatcgtcatc acttcttcac ggaggtcggt ctgtaatgaa tcacaaggac tgggattttg
     4201 ttaaccgtcg gctggtggca aaaatgttgt ctgagatgga gtatgagcag gttttccacg
     4261 cggaatctca gggcgatgac cactactgca ttaacctgcc gggagcacaa tggcgcttca
     4321 tcgctgaacg tggtatctgg ggctggctgt ggattgatgc ccaaacgttg cgctgcacgg
     4381 acgaaccagt actggcgcag acgctgctga tgcagctaaa gccggtgctg tcaatgagtg
     4441 atgccactgt tgcagagcat atgcaggatt tgtatgccac gctactgggc gacctgcaac
     4501 tgctgaaggc ccgtcgcgga ctgagtgcca gtgacctgat taatcttgat gccgatcgtc
     4561 tgcaatgcct gctgagcggt catcctaaat tcgtttttaa taaaggtcgc cgtggctggg
     4621 gcaaagaggc gctggaacga tatgcgccag agtataccaa caccttcaga ctgcactggc
     4681 tggcggtaaa acgtgaacat atgatctggc gctgcgataa cgatctggat attcagcagt
     4741 tgttgactgc cgcaatggat ccgcaggagt ttacacgctt cagtcaggtc tggcaggaaa
     4801 acgggctgga tcacaactgg ctgccgctgc cggtacatcc gtggcagtgg cagcaaaaaa
     4861 tcgccacaga cttcatcgct gattttgccg aaggcaggat ggtgtctctc ggcgagtttg
     4921 gcgaccagtg gctcgcccag cagtcgctgc gtaccctgac caacgccagc cgccggggag
     4981 ggctggatat caagctgccg ctgaccatct acaatacctc atgctaccgg gggatccctg
     5041 gcagatatat cgctgccgga ccactagctt cacgctggct gcaacaggtt tttgcgaccg
     5101 acgccaccct ggtgcaaagc ggcgcagtga tccttggtga accggctgca ggctatgtgt
     5161 cccatgaagg ctatgccgcg cttgcccggg ctccctatcg ctaccaggaa atgcttggtg
     5221 ttatctggcg ggagaatccg tgccgctggc tgaaaccgga tgaaagtcct gttctgatgg
     5281 caacactgat ggagtgcgac gaaaacaatc agccgctggc aggcgcatat atcgaccgct
     5341 ccggactgga cgctgaaacc tggcttacgc aattgttccg ggtggttgtg gttccgctgt
     5401 atcacctgct ttgccgctac ggtgtcgcgc ttattgcaca cggacaaaat ataacgctcg
     5461 ccatgaaaga gggggtacca cagcgtgttc tgctgaaaga cttccagggc gatatgcggc
     5521 tggtgaaaga agcgtttccc gaaatggact ctttgcctca ggaggtgcgt gatgtcacat
     5581 cccgtctgag tgcggactac ttaatccatg atttgcagac gggtcacttc gtgacagtac
     5641 tgcgttttat ttcgccactg atggttcgtc ttggcgtacc tgaaaggcga ttttatcaac
     5701 tgctggcagc agtgttgagt gattacatga acaaacatcc acaaatggca gagcgttttg
     5761 cgcttttctc actcttcagg ccacaaatca ttcgcgtggt gctgaacccg gtaaaactga
     5821 cctggccgga cctggatggc ggcagtcgca tgctgccgaa ttaccttgag aatctgcaaa
     5881 atccgctgtg gctggtaact caggaatatg aatcatgaaa aaaagtgtcg attttattgg
     5941 tgtcgggaca gggccattta atctcagcat tgctgcattg tcacatcaga tcgaagaact
     6001 gaactgtctc ttctttgacg aacatcctca tttttcctgg catccgggta tgctggtacc
     6061 ggattgtcat atgcagaccg tctttctgaa agatctggtc agtgcagttg cacctacaaa
     6121 tccctacagc tttgttaact atctggtgaa gcacaaaaag ttctatcgct tccttacaag
     6181 cagactacgt acagtatccc gtgaagagtt ttctgactat ctccgctggg ctgctgaaga
     6241 tatgaataac ctgtatttca gtcataccgt tgaaaatatt gatttcgaca aaaaaagtcg
     6301 attgtttctg gttcagacca gtcggggaga atattttgcc cacaatatct gccttggtac
     6361 aggaaaacaa ccttatttac caccctgtgt gaagcatgtg acacaatcct gtttccatgc
     6421 cagtgaaatg aatcttcgtc ggccggacct gagtggaaaa cggataaccg tggttggtgg
     6481 aggacagagt ggtgcagacc tgttccttaa tgcattacgc ggggaatggg gagaagcggc
     6541 ggaaataaac tgggtctcac ggcgtaataa ttttaacgca ctggatgagg ctgcttttgc
     6601 tgatgagtat tttacacctg aatatatttc aggcttctcc ggactgaagg aagatattcg
     6661 ccatcagtta ctggatgagc agaaaatgac atcggatggc atcactgccg attctttact
     6721 gaccatttat cgtgagttgt accaccgttt tgaagttctg agaaaaccaa gaaatatccg
     6781 tctgctaccc agtcgctcgg taacaactct ggaaagtagt ggtccgggct ggaggttatt
     6841 gatggaacat catctggatc agggcagaga gagcctggaa agtgatgtgg tgattttcgc
     6901 cacaggttac cgttctgcat tgccacaaat acttccctca ctgatgccct tgatcaccat
     6961 gcacgataag aacaccttta aagtgcgtga tgacttcact ctggaatgga gtggcccgaa
     7021 agaaaataac atctttgcgg tcaacgccag catgcaaact catggcatcg ccgaacccca
     7081 gctcagcctg atggcctggc gatctgcacg tattcttaat cgcgtactgg ggcgtgattt
     7141 attcgatctc agtatgccgc ccgcactgat tcagtggcgc agcggcagcc ggaaaaaacc
     7201 gcagccggag gctgcttctt taactcacta tacagcaaat attcaggaat aacgatgatg
     7261 cgcaaaaagt atatgccccg ggctcttggt ccgctgcttc ttgtcgtgct gtcaccagct
     7321 gtcgcccagc aaaacgatga taatgagatc atagtgtctg ccagccgcag caatcgaact
     7381 gtagcggaga tggcgcaaac cacctgggtt atcgaaaatg ccgaactgga gcagcagatt
     7441 cagggcggta aagagctgaa agacgcactg gctcagttaa tccccggcct tgatgtcagc
     7501 agccagagtc gaaccaacta cggtatgaac atgcgtggcc gcccgctggt tgtcctgatt
     7561 gacggtgtgc gcctcaactc ttcacgttcc gacagccgac aactggactc tgtcgatcct
     7621 tttaatatcg accatattga agtgatctcc ggcgcgacgg ccctgtacgg tggcgggagt
     7681 accggagggt tgatcaacat cgtgaccaaa aaaggtcagc cggaaaccat gatggagttt
     7741 gaggctggca caaaaagtgg ctttaacagc agtaaagatc acgatgagcg cattgccggt
     7801 gctgtctccg gcggaaatga ccatatctcc ggacgtcttt ccgtggcata tcagaaattt
     7861 ggcggctggt ttgacggtaa cggcgatgcc accctgcttg ataacaccca gaccggcctg
     7921 cagcactcca atcggctgga catcatggga accggtacgc tgaacatcga tgaatcccgg
     7981 cagcttcaac tgataacgca gtactataaa agtcagggag acgacaatta cgggcttaat
     8041 ctcgggaaag gcttttccgc catcagcggg agcagcacac catacgtcag taaggggctg
     8101 aattctgacc gcattcccgg cactgagcgg catttgatca gcctgcagta ctctgacagt
     8161 gatttcctgg gacaggaact ggtcggtcag gtttactacc gcgatgagtc gttgcggtac
     8221 tacccgttcc cgacggtaaa tgcgaataaa caggcgacgg ctttctcctc gtcacagcag
     8281 gataccgacc agtacggcat gaaactgact ctgaacagcc aactgatgga cggctggcaa
     8341 atcacctggg ggctggatgc tgagcatgag cgctttacct ccaaccagat gttcttcgat
     8401 ctggctcagg caagtgcttc cggagggctg aacaaccata agatttacac caccgggcgc
     8461 tatccgtcat atgacatcac caatctggcg gccttcctgc aatccagcta tgacattaat
     8521 gatattttta ccgttagcgg tggcgtacgc tatcagtata ctgagaacag ggtagatgat
     8581 ttcatcgact acacgcagca acagaagatt gctgccggga aggcgatatc tgccgacgcc
     8641 attcctggtg gttcggtaga ttacgataac tttctgttca atgctggtct gctgatgcac
     8701 atcaccgaac gtcagcaggc atggttcaat ttttcccagg gggtggcatt gccggatccg
     8761 gggaaatatt atggtcgcgg catctatggt gcagcagtga acggccatct tcccctgaca
     8821 aagagcgtga acgtcagcga cagtaagctg gaaggcgtga aagtcgattc ttatgaactg
     8881 ggctggcgct ttatcggtga caacctgcgg actcaaatcg cggcatatta ctcgctttcc
     8941 aataagagcg tggaaaggaa taaagatctg accatcagtg tgaaggacga caggcgccgt
     9001 atttacggcg tggaaggtgc ggtggactac ttgatcccgg atactgactg gagtaccggt
     9061 gtgaacttca atgtgctgaa aaccgagtcg aaagtgaacg gtcaatggca aaaatatgac
     9121 gtgaaggaat caagtccatc gaaagcgaca gcttacatta actgggcgcc ggaaccgtgg
     9181 agtctgcgtg tacagagcac cacttctttc gacgtaagcg atgcagaggg taacgatatt
     9241 aatggttaca ctaccgtcga ttttatcagt agttggcagc ttccggtggg aacactcagc
     9301 ttcagcgttg agaacctctt cgaccgtgac tataccactg tctggggaca gcgtgcacct
     9361 ctgtactaca gcccgggtta cggccctgct tcactgtacg actacaaagg ccggggccga
     9421 acctttggtc tgaactactc agtgctgttc tgacggggat gcttttgcaa caagggtatg
     9481 ctgtaaacaa catggtcctt ggtggccatg ttgtttacag ccagtattgt ttacagccag
     9541 tataatatac agccttaaca agatattccg tctccggaat ggactgttca ccactgaata
     9601 acattatgtc tgttttatgg atctttgact gaaatttatc gcatatcttc atttcattgt
     9661 ccgtctgaaa actaacctta caagtaagcg tcgtctcagc accgtctggc agatcctgaa
     9721 attcctgaga ggatgatctg cgcccaatat cctgagccgg aactttgctg caatgaaggc
     9781 caacgaaaaa tgggggagta tgttatagag tacttgctac agggtaaaaa gaggtacctg
     9841 tcgctggttc tccgttcagt ttaaccggga gattttctcc tgcgcctgtc ataaagtccg
     9901 gtaatggaga ttgttaatac gatggtgcac gatgcattgt cagtgcccgg cccggaggat
     9961 gcaccgtagc tacatacgga tcagggttgg caatatcgaa tggcaggcta taggcaaagt
    10021 taaagcccca tggagtagca caaaatatgc cgcacaaagg aaacggtctg aataacgcag
    10081 tgatggagaa cttcttcggc acactgcaaa atcgcatcgt ggtcgatcgc taattcgtcg
    10141 tgaatcactg aacgatgttc catgcgaaca gatattcgat catatcgaaa tattaaacat
    10201 tacccctccc cacattgggc aaaaaaaaac aaaaaacgat caattaacga tcaattagta
    10261 ttgattcaca taagctctca tcattaatga gaacattgtc acatatttac aatatagaac
    10321 tgttactttt agcaagctgt tagtaaccaa ccgttactaa taattttaat tcttaatact
    10381 ttgttcattc aataaatgag ttgagagaat atatgaataa aatatactcc cttaaatata
    10441 gtgctgccac tggcggactc attgctgttt ctgaattagc gaaaagagtt tctggtaaaa
    10501 caaaccgaaa acttgtagca acaatgttgt ctctggctgt tgcaggtaca gtaaatgcag
    10561 caaatattga tatatcaaat gtatgggcga gagactatct tgatcttgca caaaataaag
    10621 gtattttcca gcccggagca acagacgtaa caatcacttt aaaaaacgga gataaattct
    10681 ctttccataa tctctcaatt ccggattttt ctggtgcagc agcgagtggc gcagctaccg
    10741 caataggagg ttcttatagt gttactgttg cacataacaa aaagaaccct caggccgcag
    10801 aaactcaggt ttacgctcag tcttcttaca aggttgttga cagaagaaat tccaatgatt
    10861 ttgagattca gaggttaaat aaatttgttg tggaaacagt aggtgccacc ccggcagaga
    10921 ccaaccctac aacatattct gatgcattag aacgctacgg tatagtcact tctgacggtt
    10981 caaaaaaaat cataggtttt cgtgctggct ctggaggaac atcatttatt aatggtgaat
    11041 ccaaaatctc aacaaattca gcatatagcc atgatctgtt aagtgctagt ctatttgagg
    11101 tcacccaatg ggactcatac ggcatgatga tttataaaaa tgataaaaca tttcgtaatc
    11161 ttgaaatatt cggagacagc ggctctggag catacttata tgataacaaa ctagaaaaat
    11221 gggtattagt cggaacaacc catggtattg ccagcgttaa tggtgaccaa ctgacatgga
    11281 taacaaaata caatgataaa ctggttagtg agttaaaaga tacctatagt cataaaataa
    11341 atctgaatgg caataatgta accattaaaa acacagatat aacattacac caaaacaatg
    11401 cagataccac tggtactcaa gaaaaaataa ctaaagacaa agatattgtg ttcacaaatg
    11461 ggggaaatgt tctgtttaag gataatttgg attttggtag cggtggtatt atctttgacg
    11521 aaggccatga atataacata aacggtcagg gatttacatt taaaggagca ggaattgata
    11581 tcggaaaaga aagcattgta aactggaatg cattgtattc cagtgatgat gttttacaca
    11641 aaataggccc cggtactctg aatgttcaaa aaaaacaggg ggcaaatata aagataggtg
    11701 aaggaaatgt tattcttaat gaagaaggaa catttaacaa tatatacctt gcaagcggaa
    11761 atggtaaggt aatactaaat aaagataatt cccttggcaa tgatcaatat gcggggatat
    11821 tttttactaa acgtggtggc acgctagatt taaatggaca caatcagact tttactagaa
    11881 ttgccgccac tgacgatgga acaacaataa ctaactcaga tacaacgaaa gaagccgttc
    11941 tggcaatcaa taacgaagac tcctacatat atcatgggaa cataaatggc aatataaaac
    12001 taacgcacaa tattaattct caggataaga aaactaatgc aaaattaatt ctggatggta
    12061 gtgtcaacac aaaaaatgat gttgaagtca gtaatgccag tcttaccatg caaggccatg
    12121 caacagagca tgcaatattc agaagcacag cgaatcattg ctccctggta tttctttgtg
    12181 gaacggactg ggtcaccgtt ttgaaagaaa cagagagttc atataataaa aagttcaatt
    12241 ctgattacaa aagtaataat cagcagacct catttgatca gcctgactgg aaaaccgggg
    12301 tgtttaaatt tgatacatta cacctgaaca atgctgactt ttcaatatca cgcaatgcca
    12361 atgttgaagg aaatatatca gcaaataaat cagctatcac aatcggcgat aaaaatgctt
    12421 acattgataa tcttgcaggg aaaaatatta ctaataatgg ttttgacttc aaacaaacta
    12481 tcagtactaa tctatccata ggagaaacta aatttacagg tggcatcact gcacataaca
    12541 gccaaatagc cataggtgat caagctgtag ttacacttaa tggtgcaacc tttctgaata
    12601 atactcctat aagtatagat aaaggagcaa aagttatagc acaaaattcc atgttcacaa
    12661 caaaaggtat tgatatctcc ggtgaactga ctatgatggg aatccctgaa cagaacagta
    12721 aaactgtaac gccgggtctc cactacgctg ctgatggatt caggctgagt ggtggaaatg
    12781 caaatttcat tgccagaaat atggcatctg tcaccggaaa tatttatgct gatgatgcag
    12841 caaccattac tctgggacag cctgaaactg aaacaccgac tatatcgtct gcttatcagg
    12901 catgggcaga gactcttttg tatggctttg ataccgccta tcgaggcgca ataacagccc
    12961 ccaaagctac agttagcatg aataatgcga tctggcatct aaatagccag tcatcaatta
    13021 atcgtctaga aacaaaagac agtatggtgc gttttactgg tgacaatggg aagtttacaa
    13081 cccttacagt ggacaacctt actatagatg acagtgcatt tgtgctgcgt gcaaatctgg
    13141 cccaagcaga tcagcttgtt gtcaataaat cgttgtctgg taaaaacaac cttctgttag
    13201 tcgacttcat tgagaaaaat ggaaacagca acggactgaa tatcgatctg gtcagcgcac
    13261 caaaaggaac tgcagtagat gtctttaaag ctacgactcg gagtattggc ttcagtgatg
    13321 taacaccggt tatcgagcaa aagaacgata cagacaaagc aacatggact ctgatcggct
    13381 ataaatctgt ggccaacgcc gatgcggcta aaaaggcaac attactgatg tcaggcggct
    13441 ataaagcctt ccttgctgag gtcaacaacc ttaacaaacg tatgggtgat ctgcgtgaca
    13501 ttaacggtga gtccggtgca tgggcccgaa tcatgagcgg aaccgggtct gccggcggtg
    13561 gattcagtga caactacacc cacgttcagg tcggtgcgga taacaaacat gaactcgatg
    13621 gccttgacct cttcaccggg gtgaccatga cctataccga cagccatgca ggcagtgatg
    13681 ccttcagtgg tgaaacgaag tctgtgggtg ccggtctcta tgcctctgcc atgtttgagt
    13741 ccggagcata tatcgacctc atcggtaagt acgttcacca tgacaacgag tataccgcaa
    13801 ctttcgccgg ccttggcacc agagactaca gctcccactc ctggtatgcc ggtgcggaag
    13861 tcggttaccg ttaccatgta actgactctg catggattga gccgcaggcg gaacttgttt
    13921 acggtgctgt atccgggaaa cagttctcct ggaaggacca gggaatgaac ctcaccatga
    13981 aggataagga ctttaatccg ctgattgggc gtaccggtgt tgatgtgggt aaatccttct
    14041 ccggtaagga ctggaaagtc acagcccgcg ccggccttgg ctaccagttt gacctgtttg
    14101 ccaacggtga aaccgtactg cgtgatgcgt ccggtgagaa acgtatcaaa ggtgaaaaag
    14161 acggtcgtat gctcatgaat gttggtctca atgccgaaat tcgcgataat cttcgcttcg
    14221 gtcttgagtt tgagaaatcg gcatttggta aatacaacgt ggataacgcg atcaacgcca
    14281 acttccgtta ctctttctga taacagcccg ggccgcgttt gcggcccttc ttctaccgga
    14341 gagaatatgt attaccctgt gacagactat atcgctcttg ctctcattat tagctttctt
    14401 tttctgacat tatttatctg cctgttatgc cttaaacatg agcgaataaa aaaagaaact
    14461 atcaggcaaa aaaatgcaca tattctggat catggctgga atgcaactga gttctcatgg
    14521 ttccgatacg gacagtataa cgaaacgggt atttacatct cgatcgagaa gaccattatc
    14581 attacaatca ccgtttctgg tgggt
//
LOCUS       DADLSA010000097        14336 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.097, whole genome shotgun sequence.
ACCESSION   DADLSA010000097 DADLSA010000000
VERSION     DADLSA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.097"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(93..218)
                     /gene="ecnA"
                     /locus_tag="JN691_002858"
     CDS             complement(93..218)
                     /gene="ecnA"
                     /locus_tag="JN691_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY7244263.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(270..836)
                     /gene="efp"
                     /locus_tag="JN691_002859"
     CDS             complement(270..836)
                     /gene="efp"
                     /locus_tag="JN691_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY7244264.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            878..1906
                     /gene="epmB"
                     /locus_tag="JN691_002860"
     CDS             878..1906
                     /gene="epmB"
                     /locus_tag="JN691_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY7244265.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDKLLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            2301..3170
                     /locus_tag="JN691_002861"
     CDS             2301..3170
                     /locus_tag="JN691_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244266.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(3373..3726)
                     /locus_tag="JN691_002862"
     CDS             complement(3373..3726)
                     /locus_tag="JN691_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAY7244267.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(3864..5519)
                     /gene="groL"
                     /locus_tag="JN691_002863"
     CDS             complement(3864..5519)
                     /gene="groL"
                     /locus_tag="JN691_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010427772.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAY7244268.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMGGMM"
     gene            complement(5563..5856)
                     /locus_tag="JN691_002864"
     CDS             complement(5563..5856)
                     /locus_tag="JN691_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAY7244269.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            6132..7388
                     /gene="yjeH"
                     /locus_tag="JN691_002865"
     CDS             6132..7388
                     /gene="yjeH"
                     /locus_tag="JN691_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAY7244270.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(7404..7880)
                     /gene="fxsA"
                     /locus_tag="JN691_002866"
     CDS             complement(7404..7880)
                     /gene="fxsA"
                     /locus_tag="JN691_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAY7244271.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            8217..9653
                     /gene="aspA"
                     /locus_tag="JN691_002867"
     CDS             8217..9653
                     /gene="aspA"
                     /locus_tag="JN691_002867"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAY7244272.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            9771..11072
                     /locus_tag="JN691_002868"
     CDS             9771..11072
                     /locus_tag="JN691_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY7244273.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            11188..11526
                     /gene="cutA"
                     /locus_tag="JN691_002869"
     CDS             11188..11526
                     /gene="cutA"
                     /locus_tag="JN691_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAY7244274.1"
                     /translation="MLDEKSSNTASVVVLCTAPYEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            11502..13199
                     /locus_tag="JN691_002870"
     CDS             11502..13199
                     /locus_tag="JN691_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAY7244275.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            13236..13811
                     /locus_tag="JN691_002871"
     CDS             13236..13811
                     /locus_tag="JN691_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7244276.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            13918..13993
                     /locus_tag="JN691_002872"
     tRNA            13918..13993
                     /locus_tag="JN691_002872"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13951..13953,aa:Phe,seq:gaa)
BASE COUNT         3492 a         3676 c         3524 g         3644 t
ORIGIN      
        1 aatgttattt atgtttgcct atagcaaatt aataatagac aacatcgcca aaaatgatgg
       61 aaatctgatg atttttcgga agagaagaaa aattagctgg cagcgcgaga gatggagttg
      121 ccgagatgtt tgatgtcttc gccgaaaccg caagcggtgt tacagcccgt gagcagcgtg
      181 ctggcaagca aaacaagaac gataaggcgt ttcatcatct ctgacctgaa taagtgatgg
      241 tgcagcctgc aggccgcacc acaaccgcat tacttcacgc gagagacgta ttcaccagag
      301 cgggtatcca ctttgatgac ttcgccgatt tgtacgaaca gcggaacttt aaccacagcg
      361 ccagtagaca gggtagccgg tttgccgcca gtacctgcgg tatcaccttt caggcccgga
      421 tcggtatcaa cgatttccag ttcaacgaag ttcggcggag taacggagat cggctgacca
      481 ttccacagag ttacgataca ctctgcctga tccagcagcc atttagcgtt gtcgccaatt
      541 gcttttgcat cagcagacag ctgctcgaaa gtttcgttgt tcatgaagtg ccagaactca
      601 ccgtcgttgt acaggtaagt caggttcata tcgacaacat cagcgccttc agcggaatca
      661 gtagatttga aggttttttc tacgcgagta ccggtcagca gacgacgcag tttaacgcga
      721 gcaaatgcct ggcctttacc cggttttacg aattcactcg cttcaaccgc gtaaggttcg
      781 ccgtctaaca tgattttaag accagcacga aaatcgttgc tatagtacgt tgccataagg
      841 ccctctgaaa tttgttaatt ggtagctaag ccacaaaatg gcgcatattg taaccctaaa
      901 taccccatcc agagaagatt ggttaacgca acttgccgat gttgtgaccg atcctgataa
      961 acttctgcgt cttttgaata tagacgcgga cgaaaaactg ttggccggac gcagcgccaa
     1021 aaagctgttt gccctgcgtg tgccccgctc atttatcgat cgcatggaga aaggcaatcc
     1081 ggacgatcct cttttgcgtc aggtacttac ctcgcaagat gagtttgtcg tcgcgtccgg
     1141 attctccacc gacccactgg aagaacagca cagcgtagtg cctggtttgt tgcataaata
     1201 ccacaaccgg gcgcttttgc tggtcaaagg cggctgcgcg gtaaattgcc gctattgctt
     1261 ccgtcgccac ttcccctatg ccgaaaatca gggcaacaag cgtaactggc agactgcact
     1321 tgagtatgtt gctgcgcatc cggaactgga cgagatgatt ttctccggcg gcgatccgct
     1381 gatggcgaaa gatcacgagc tggactggtt gctcacacaa ctggaagcca tcccgcatat
     1441 caaacgtctg cggattcaca gccgtctgcc gattgtgatc ccggcacgta tcaccgaggc
     1501 gctggttgaa cgctttgccc gttctacgct gcaaatcttg ctggtgaatc acatcaatca
     1561 tgccaatgag gtagatgaaa cgttccgtca ggcgatggct aaattgcgcc gtgtcggtgt
     1621 caccctgctt aaccagagcg ttctgttacg tggtgtgaac gataacgcac aaacgttggc
     1681 aaacctgagt aatgcgttgt tcgatgccgg cgtgatgccg tattacctgc atgtgctaga
     1741 taaagtacag ggcgcggcgc attttatggt gagtgatgac gaagcacggc agattatgcg
     1801 tgagttgctg acattggtgt cgggatatct ggtgccgaaa ctggcgcgag aaattggcgg
     1861 cgaacccagc aaaacgccgc tggatctcca gctacgccag cagtaactga ttaaacaggg
     1921 aagttaaaca ttaaaattgc ctgatgcgct acgcttatca ggcctacatc gtgcattgaa
     1981 attgaatgga tttgcaatga actgtaggcc ggataaggcg tttacgccgc atccggcaag
     2041 agcaacaaac gcaagtacag caacctgtca ttaaactgag aaatttaatc tttgcacgtt
     2101 gcataacctt taattattca tatgcattgc tgttgttact ttttatatag ctaacaccaa
     2161 taattaacct ttcttttctg tttatataga gtgagcacgg ctatttatgt tattttaaca
     2221 actattacta ccaagcccat ttatctggct tataaattaa aattaattat ttaattgaga
     2281 aatggttagg gagaacctac atggccataa gcatcaaggg tgttaatacc ggtgttattc
     2341 gcaagagtaa taatttcatt gcactggcgc tgaaaattaa agaacctcgc aataaagaat
     2401 cgttgttctt tatgtctgtc atggaactgc gcgacctgct gattgcgctg gaaagtcgtc
     2461 tgcatcaaaa gcataaactg gatgctgctg cacgccttca gtatgagcaa gctcgcgata
     2521 aagtcattaa gaaaatggca gaaaacatcc cagaaattct ggttgatgag cttaaaaatg
     2581 ccgatattaa tcgtcgggtt aacacgctgg aacttaccga taatcaaggc gaaaatctta
     2641 ccttcgtatt aacgctacat gacggtagca aatgtgagtt agtcgtcaat gagttgcaga
     2701 ttgaaatgct ggcgcgagca atcattcatg caattaataa tgcggaaatg cgtgaactgg
     2761 cattacgtat tacctcactg ttagatttcc tgccgttata cgacgttgac tgccaggaca
     2821 atggcaatct ggaatatgac acctattccc aaccggaatg gaaacataac ttgttcgacc
     2881 attatctggc ggtgctttac cgctttaaag atgaaagtgg caaagaacaa tttagcggcg
     2941 cagtggtgaa aacgcgtgaa gcaacgccgg gtaaagaaat agaggcgatc actcgccgaa
     3001 tgcttgattt cagcccacga ctgaaaaaac tcgcgggtgt gccatgtcag gtctatgttc
     3061 gcaccgtagc ggcgaataac gcccaaccgc taacccagga tcaatgctta cgcgcgctgc
     3121 atcacttacg tgtacaatcc accagcaaaa ctgcaccaca ggcgaaataa tctcatcttt
     3181 gctaatgcga ggctggagcc atccagcctg aaatgactga taacgtgatc gttggtcatg
     3241 ccggatgcgg cgtaaacgcc ttatccggcc tacaaattct caagcattca atcagttgca
     3301 atgcctgctg taggcctgat aagcgaagcg catcaggcaa taatacgttt gtcacccagc
     3361 ctctggatca catcagttcg ggcacttata aacctgaccg ataatctggc tatccgtcgg
     3421 gacaaaactg gacaacattc cctgcgacgg gctgctgatg ccataaatca cgttaccgcc
     3481 cattgcagcc gcctggttgc gcagatcgtt tgctgcgccg cgcatagaac cgccctcttc
     3541 tccgtgttgc ccggaaagcc agttgctttg cttacctgtc gcagtaccaa tcagctggca
     3601 ctctgcgcct ggctgctcgt ccacaatgcg tacactctga ccggcagcac tcaattcgtt
     3661 gctggagcta caacccgcca tcagtagcgc ggcaccgaca atccctgcta agtattttac
     3721 gtgcatgtta ttccccataa tcaatgagct ggacgccact cgcgtcgtcc gtgtctgaat
     3781 cttatactag aaagatgacc aaaagaaaaa cccccagaca tttctgcccg ggggtttgtt
     3841 tatttctgcg aagtgcaggg caattacatc atgccgccca tgccacccat gccgcccata
     3901 ccgcccatac cgccagcagc gcctaagtca gctgcatcgt ttttcggcag gtcggtaacc
     3961 atgcattcgg tggtgatcat caggccagcc acagaagctg cgtactgcag agcagaacgg
     4021 gttactttgg ttgggtccag gatacccatg tcgatcatgt tgccgtattc ttcggttgct
     4081 gcgttgtaac cgtagttgcc gtcgccgcct ttaacggtgt tagcaacaac agacggttct
     4141 tcgccgcagt tcaggacgat ctgacgcagc ggagcttcca ttgcacgcag tgcaactttg
     4201 atacccacgt tctggtcttc gttctgacca cgcaggtcag ccagtttaga cgctacgcgg
     4261 atcagcgcaa caccaccacc agcaaccacg ccttcttcta ccgcagcacg ggtcgcgtgc
     4321 agggcatctt caacgcgtgc ttttttctct ttcatttcaa cttcggtagc agcacctact
     4381 ttgataactg caacgccgcc tgccagtttc gctacgcgct cctgcagttt ttcacggtcg
     4441 tagtcagaag ttgcttcttc aatctgctga cggatctgag caacacggcc ctggattgca
     4501 gcttcttcac ccacgccatc gatgatagtg gtggtgtctt tgttgatcac aacacgttta
     4561 gcctgaccca ggtcttccag ggttgctttt tccagctcca taccgatctc ttcagagatt
     4621 acggtaccgc cagtcagggt tgcgatatcc tgcagcatag ctttacgacg atcgccgaag
     4681 cccggagctt taaccgcagc aactttcacg atgccacgca tggtgttaac aaccagagtt
     4741 gccagcgctt cgccttcaac atcttcagcg atgatcagca gcggtttgcc tgctttggca
     4801 acagcttcca gaaccggcag catttcgcgg atgttggaga ttttcttgtc agccagcagg
     4861 atgaacgggc tttccagttc tactgcgcca gtttccggct tgttgatgaa gtaaggagac
     4921 aggtagccac ggtcgaactg cataccttca accacgtcca gttcgtcctg cagaccggta
     4981 ccgtcttcaa cggtgataac gccttcttta ccgactttgt ccatcgcttc agcgatcagt
     5041 ttacctacgg tttcgtcgga gttagcggag atggtaccaa cctgagcaat cgctttagaa
     5101 tcagagcacg gtacggacag cgctttcagt tcttcaactg cagcggtaac cgctttgtcg
     5161 ataccacgtt tcaggtccat cgggttcatg cccgcagcaa cagctttcag accttcagtg
     5221 atgatagcct gagccagtac ggttgcagtg gtggtaccgt cgcctgcagc gtcgttcgct
     5281 ttggaggcaa cttctttcac catctgcgca cccatatttt cgaacttgtc ttccagttcg
     5341 atttcacgag caacggaaac accatctttg gtgatggtcg gtgcaccgaa agatttatcc
     5401 agaactacgt tacggccttt tggaccgagg gtaactttca ctgcatctgc cagtacgttt
     5461 acgccgcgca gcattttcac acgagcgtcg ttaccgaatt ttacgtcttt agctgccatt
     5521 atctttattc cttaaattcg tatgttctgt gtcgtgcgcg gattacgctt caacaattgc
     5581 cagaatgtcg ctttcggaca tgatcaacac ttcttcattg tcgatcttct cggatttcac
     5641 accgtagcca tcgttgaaaa taacgatgtc gccaactttc acgtccagcg gcttcacttc
     5701 gccattttca aggatacggc cattgccgac agccagcact tcgccgcggg tggatttagc
     5761 cgctgcagag ccggtcagaa cgatgccgcc agcagattta gtttcaactt ctttacgctt
     5821 gacgatcacg cgatcatgca atggacgaat attcattgat aactctcctt tgagaaagtc
     5881 cgtatctgtt atgggtgacg ccggagctta cgtggtttcc cggctggtga ccagagaaat
     5941 ggggatgagg cttcgcccct tcaaggggga aaaagaaaaa aaattctgat cagcacaaaa
     6001 tcgggtgaaa accctgattc acctcacatt tcatcacaat ttcttcatcg ccgtcataag
     6061 cgaatctgat tgtgctacca tcgaaaatct acgcagttgc ccaaaatttg ggcgcaatcg
     6121 gatatcaacc catgagtgga ctcaaacaag aactggggct ggcccagggc attggcctgc
     6181 tatcgacgtc attattaggc actggcgtgt ttgccgttcc tgcgttagct gcactggtag
     6241 caggcaataa cagcctgtgg gcgtggcccg ttttgattat cttagtgttc ccgattgcga
     6301 ttgtgtttgc gattctgggt cgccactatc ccagcgcagg cggcgtcgcg cacttcgtcg
     6361 gtatggcgtt tggttcgcgg cttgagcgag tcaccggctg gctgttttta tcggtcattc
     6421 ccgtgggttt gcctgccgca ctacaaattg ccgccggatt cggccaggca atgtttggct
     6481 ggcatagctg gcaactgttg ttggcagaac tcggtacgct ggcgttggtg tggtatatcg
     6541 gtactctcgg tgccagttcc agtgctaatc tacaaaccgt tattgccgga cttatcgtcg
     6601 cactgattgt cgctatctgg tgggcgggcg atatcaaacc tgcgagtatc cccttccccg
     6661 cgccaggaaa tatcgaactt accgggttat ttgctgcgtt atcagtgatg ttctggtgtt
     6721 ttgtcggtct ggaagcattt gcccatcttg cctcggaatt taaaaatcca gagcgtgatt
     6781 ttcctcgtgc tttgatgatt ggtctgctgc tggcaggatt agtctactgg ggctgtacgg
     6841 tagtcgtctt acacttcgac gcctatggtg aacaaatggc ggcggcagca tcgcttccaa
     6901 aaattgtagt gcagttgttc ggtgtaggag cgttatggat tgcctgcgta attggctatc
     6961 tggcctgctt tgccagtctt aacatttata tacagagctt cgcccgcttg gtctggtcgc
     7021 aggcgcaaca taatcctgac cactacctgg cacgcctctc ttctcgccat atcccgaata
     7081 atgccctcaa tgcggtgctt ggctgctgcg tggtgagcac gttggtaatt catgccttag
     7141 agatcaatct ggacgctctt attatttatg ccaatggcat ctttattatg atttatctgt
     7201 tatgcatgct ggcaggctgt aaattattgc aaggtcgtta tcgactactg gcggtggttg
     7261 gcgggctgtt atgcgttctg ttactggcaa tggtcggctg gaaaagtctc tatgcgctga
     7321 tcatgctggt ggggttatgg ctgtttctgc caaaacgaaa aacgccggaa aatggcataa
     7381 ccacataatc cggcgtttcg acattaatcc tggcgatcgt ctttatgatc aaggcggtcg
     7441 cggtcatcat cctttcgctg atactcacca tcaaaagtat taccgccacc ggtcccggcg
     7501 ctaaaaccgc cgcctggcat gcgagaaaag cgcaaatgtg gcatcaactt cactgtcaga
     7561 tgcttttgca ccggcggcaa taaaagcaga agaccgagga agtcggtgaa aaagcccggt
     7621 aataaaagca gcaaaccggc aataatcagc gaaacacttt taatcatctc cgccgcaggg
     7681 ttttcacccg ccgccatttt ttgctgcatc agcacgaaat tcttaaagcc ctggttacgt
     7741 accagtgaca taccgataac tgacgtgaat ataaccagca cgagggtcag caataccccc
     7801 aatacatggg caacctgaat aaagattgaa atctcaatat agacataaag gaaaatggca
     7861 ataaaaggta accagcgcaa aggtttctcc tgtaatagca gccggttaaa cccggctacc
     7921 tgaatgggtt gcgaatcgcg ttcagcttat attgtggtca ttagcaaaat ttcaagatgt
     7981 ttgcgcaact atttttggta gtaatcccaa agcggtgatc tatttcacaa attaataatt
     8041 aaggggtaaa aaccgacact taaagtgatc cagattacgg tagaaatcct caagcagcat
     8101 atgatctcgg gtattcggtc gatgcagggg ataatcgtcg gtcgaaaaac attcgaaacc
     8161 acatatattc tgtgtgttta aagcaaatca ttggcagctt gaaaaagaag gttcacatgt
     8221 caaacaacat tcgtatcgaa gaagatctgt tgggtaccag ggaagttcca gctgatgcct
     8281 actatggtgt tcacactctg agagcgattg aaaacttcta tatcagcaac aacaaaatca
     8341 gtgatattcc tgaatttgtt cgcggtatgg taatggttaa aaaagccgca gctatggcaa
     8401 acaaagagct gcaaaccatt cctaaaagtg tagcgaatgc catcattgcc gcatgtgatg
     8461 aagtcctgaa caacggaaaa tgcatggatc agttcccggt agacgtctac cagggcggcg
     8521 caggtacttc cgtaaacatg aacaccaacg aagtgctggc caatatcggt ctggaactga
     8581 tgggtcacca gaaaggtgaa tatcagtacc tgaacccgaa cgaccatgtt aacaaatgtc
     8641 agtccactaa cgacgcctac ccgaccggtt tccgtatcgc agtttactct tccctgatta
     8701 agctggtaga tgcgattaac caactgcgtg aaggctttga acgtaaagct gtcgaattcc
     8761 aggacatcct gaaaatgggt cgtacccagc tgcaggacgc agtaccgatg accctcggtc
     8821 aggaattccg cgctttcagc atcctgctga aagaagaagt gaaaaacatc caacgtaccg
     8881 ctgaactgct gctggaagtt aaccttggcg caacagcaat cggtactggt ctgaacacgc
     8941 cgaaagagta ctctccgctg gcagtgaaaa aactggctga agtcactggc ttcccatgcg
     9001 taccggctga agacctgatc gaagcgacct ctgactgcgg cgcttatgtt atggttcacg
     9061 gcgcgctgaa acgcctggct gtgaagatgt ccaaaatctg taacgacctg cgcttgctct
     9121 cttctggtcc acgtgccggc ctgaacgaga tcaacctgcc ggaactgcag gcgggctctt
     9181 ccatcatgcc agctaaagta aacccggttg ttccggaagt ggttaaccag gtatgcttca
     9241 aagtcatcgg taacgacacc actgttacca tggcagcaga agcaggtcag ctgcagttga
     9301 acgttatgga accggtcatt ggccaggcca tgttcgaatc cgttcacatt ctgaccaacg
     9361 cttgctacaa cctgctggaa aaatgcatta acggcatcac tgctaacaaa gaagtgtgcg
     9421 aaggttacgt ttacaactct atcggtatcg ttacttacct gaacccgttc atcggtcacc
     9481 acaacggtga catcgtgggt aaaatctgtg ccgaaaccgg taagagtgta cgtgaagtcg
     9541 ttctggaacg cggtctgttg actgaagcgg aacttgacga tattttctcc gtacagaatc
     9601 tgatgcaccc ggcttacaaa gcaaaacgct atactgatga aagcgaacag taatcgtaca
     9661 gggtagtaca aataaaaaag gcacgtcaga tgacgtgcct tttttcttgt gagcagtaac
     9721 ttaaaaataa caatctaata tcaacttgtt aaaaaacaag gaaggctaat atgctagttg
     9781 tagaactcat catagttttg ctggcgatct tcttgggcgc cagattgggg ggaataggta
     9841 ttggttttgc aggcggattg ggggtgctgg ttcttgccgc tattggcgtt aaacccggta
     9901 acatcccgtt cgatgtcatt tccattatca tggcggttat cgccgctatt tctgccatgc
     9961 aggttgctgg cggtctggac tatctggttc atcagacaga aaagctgctg cgccgtaacc
    10021 cgaaatacat cacgatcctc gcaccgatcg tgacctattt cctgactatc tttgctggta
    10081 ctggcaacat ctctctggcg acactgccag ttatcgctga agttgcgaag gaacaaggcg
    10141 ttaaaccttg ccgtccgctc tctactgcag tggtatccgc gcagattgcg atcaccgcat
    10201 cgccaatctc agcggcagtg gtttacatgt cttccgtgat ggaaggtcat ggcatcagct
    10261 acctccatct gctctccgtg gtcatcccgt ccaccctgct ggcggttctg gtgatgtcct
    10321 tcctggtcac tatgctgttc aactccaaac tctctgacga tccgatttat cgcaagcgtc
    10381 tggaagaggg cttggttgaa ctgcgcggtg aaaagcagat tgaaatcaaa tccggtgcaa
    10441 aaacgtccgt ctggctgttc ctgctgggcg tagttggcgt ggttatctat gcaatcatca
    10501 acagcccaag catgggtctg gttgaaaaac cgctgatgaa caccaccaac gcaatcctga
    10561 tcatcatgct cagcgttgca actctgacca ccgttatctg taaagtcgat accgacaaca
    10621 tcctcaactc cagcaccttc aaagcaggta tgagcgcctg tatttgtatc ctgggtgttg
    10681 cgtggctggg cgatactttc gtttccaaca acatcgactg gatcaaagat accgctggtg
    10741 aagtgattca gggtcatccg tggctgctgg ccgtcatctt cttctttgct tctgctctgc
    10801 tgtactctca ggctgcaacc gcaaaagcac tgatgccgat ggctctggca ctgaacgttt
    10861 caccgctgac cgctgttgct tctttcgctg cggtgtctgg tctgttcatt ctgccgacct
    10921 acccgacgct ggttgctgcg gtacagatgg atgacacggg tactacccgt atcggtaaat
    10981 tcgtcttcaa ccatccgttc ttcatcccgg gtactctggg tgttgccctg gccgtttgct
    11041 tcggcttcgt gctgggtagc ttcatgctgt aatgatcaat cgcggggcgt tcacgccccg
    11101 ctttctttcc cgccgactaa catccttccc ccgtccgttg tatagtgacc tctctcttgc
    11161 ggttccatct gttcttgcga ggtgtttatg cttgatgaaa aaagttcgaa taccgcgtct
    11221 gtcgtggtgc tatgtaccgc accgtatgaa gcgacagccc aggatttagc cgccaaagtg
    11281 ctggcggaaa aactggcggc ctgcgcgacc ttgatccccg gcgctacctc tctctattac
    11341 tgggaaggta agctggagca agaatacgaa gtgcagatga ttttaaaaac taccgtatct
    11401 caccagcagg cactgctgga atgcctgaag tctcatcatc catatcaaac cccggaactt
    11461 ctggttttac ctgttacaca cggagacaca gattacctct catggctcaa cgcatcttta
    11521 cgctgattct gctactttgc agcacttccg tttttgccgg attattcgac gcgccgggac
    11581 gttcacaatt tgtccccgcg gatcaagcct ttgcttttga ttttcagcaa aaccaacatg
    11641 accttaatct gacctggcag atcaaagacg gttactacct ctaccgtaaa cagatccgca
    11701 ttacgccgga acacgcgaaa attgccgacg tgcagctgcc gcaaggcgtc tggcatgaag
    11761 atgagtttta cggcaaaagc gagatttacc gcgatcggct gacgcttccc gtaaccatca
    11821 accaggcgag tgcgggagcg acgttaactg tcacctacca gggctgtgct gatgccggtt
    11881 tctgttatcc gccagaaacc aaaaccgttc cgttaagcga agtggtcgcc aacaacgcag
    11941 cgtcacagcc tgtgtctgtt tcgcagcaag agcagcacac cgcgcaattg cccttttccg
    12001 cgctctgggc gttgttgatc ggtattggta tcgcctttac gccatgcgtg ctgccaatgt
    12061 acccactgat ttctggcatc gtgctgggtg gtaaacagcg gctctccact gccagagcat
    12121 tgttgctgac ctttatttat gtgcagggga tggcactgac ttacacggcg ctgggtctgg
    12181 tagttgccgc cgcagggtta cagttccagg cggcgctaca gcacccatac gtgctcattg
    12241 gcctcgccat cgtctttacc ttgctggcga tgtcaatgtt tggcttgttt actctacaac
    12301 tcccctcttc gctgcaaaca cgtctcacgt tgatgagcaa tcgccaacag ggcggctcac
    12361 ctggcggtgt gtttgttatg ggggcgattg ccggactgat ctgttcacca tgcaccaccg
    12421 caccgcttag cgcgattctg ctgtatatcg cccaaagcgg gaacatgtgg ctgggcggcg
    12481 gcacgcttta tctctatgcg ttgggcatgg gcctgccgct gatgctaatt accgtctttg
    12541 gtaaccgctt gctgccgaaa agcggcccgt ggatggaaca agtcaaaacc gcgtttggtt
    12601 ttgtgatcct cgcactgccg gtcttcctgc tggagcgagt gattggtgat gtatggggat
    12661 tacgcttgtg gtcggcgctg ggtgtcgcat tctttggctg ggcctttatc accagcctac
    12721 aggctaaacg cggctggatg cgcgtggtgc aaataatcct gctggcagcg gcattggtta
    12781 gcgtgcgccc acttcaggat tgggcatttg gtgcgacgca taccgcgcaa actcagacgc
    12841 atctcaactt tacacaaatc aaaacggtag atgagttaaa tcaggcgctc gttgaagcca
    12901 aaggcaaacc ggtgatgtta gatctttatg ccgactggtg cgtcgcctgt aaagagtttg
    12961 agaaatacac cttcagcgac ccgcaggtgc aaaaagcgtt agcagacacg gtcttacttc
    13021 aggccaacgt cacggccaac gacgcacaag atgtggcgct gttaaagcat cttaatgtcc
    13081 ttggcctacc gacaattctc ttttttgacg gacaaggcca ggagcatcca caagcacgcg
    13141 tcacgggctt tatggatgct gaaaccttca gcgcacattt gcgcgatcgc caaccgtgaa
    13201 cgacacttgc agtgggaaag acggaggaaa atagcgtgca acgtgaagat gtactgggag
    13261 aagccctgaa attattagaa ttacaaggga ttgccaacac cacgctggag atggttgctg
    13321 aacgtgtgga ttatccactg gacgagctac gccgcttctg gccagacaaa gaggcaatcc
    13381 tctacgatgc gctgcgttat cttagccaac agatagatgt ctggcgtcgt cagctgatgc
    13441 tggacgaaac gcaaaccgcc gaacaaaagc tgctggcacg ttatcaggcg ttatcggagt
    13501 gcgttaaaaa caaccgctat ccgggctgtc tatttatcgc tgcctgtacg ttttatcccg
    13561 atcctggcca ccctattcat caactggccg atcagcaaaa aagcgcggcc tacgatttca
    13621 cccacgaact gttaaccacg ctggaagttg acgatccggc gatggtagca aagcagatgg
    13681 aactggtgct ggaaggctgt ttaagccgaa tgctggtgaa tcgtagccag gcggatgtcg
    13741 acactgcaca tcggctggcg gaagatatcc tgcgcttcgc ccgctgccgc cagggtggtg
    13801 cattgacctg acagaaacac agaaaagaag cgatttgccg caatcttaag cagttgaatc
    13861 gcttttactg aaattaggtt gacgagatgt gcagattacg gtttaatgcg ccccgttgcc
    13921 cggatagctc agtcggtaga gcaggggatt gaaaatcccc gtgtccttgg ttcgattccg
    13981 agtccgggca ccaaattcat atcaacggac ctccacggag gtccgttttt cgtttcagga
    14041 cgccacgatt taagcgttct gcctccaaat caattctacc gaactcaacc agattctccc
    14101 cacatcacca gcaatttgcg agcatatccc aatttgggaa aatttgttcc tgagctatag
    14161 cactaactga cgtgaaatgt cgtgcggccc cgtgatgctg ttgaacgtca aatgacgtca
    14221 tcaggagcgt aacgcatcca taaagcacaa caccggtctg aacgccgact gatgagattt
    14281 tctgaatgag aacaaagaga aatgtgtcag tccgcttgct catgcaaaga ctaaca
//
LOCUS       DADLSA010000098        13849 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.098, whole genome shotgun sequence.
ACCESSION   DADLSA010000098 DADLSA010000000
VERSION     DADLSA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.098"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(310..1014)
                     /locus_tag="JN691_002873"
     CDS             complement(310..1014)
                     /locus_tag="JN691_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY7244277.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1151..1603)
                     /gene="moaE"
                     /locus_tag="JN691_002874"
     CDS             complement(1151..1603)
                     /gene="moaE"
                     /locus_tag="JN691_002874"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAY7244278.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1605..1850)
                     /gene="moaD"
                     /locus_tag="JN691_002875"
     CDS             complement(1605..1850)
                     /gene="moaD"
                     /locus_tag="JN691_002875"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAY7244279.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1843..2328)
                     /gene="moaC"
                     /locus_tag="JN691_002876"
     CDS             complement(1843..2328)
                     /gene="moaC"
                     /locus_tag="JN691_002876"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAY7244280.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2331..2843)
                     /gene="moaB"
                     /locus_tag="JN691_002877"
     CDS             complement(2331..2843)
                     /gene="moaB"
                     /locus_tag="JN691_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAY7244281.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREFEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2865..3854)
                     /gene="moaA"
                     /locus_tag="JN691_002878"
     CDS             complement(2865..3854)
                     /gene="moaA"
                     /locus_tag="JN691_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAY7244282.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDSIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3842..3986)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4251..5159
                     /locus_tag="JN691_002879"
     CDS             4251..5159
                     /locus_tag="JN691_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAY7244283.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASTMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5351..7372)
                     /gene="uvrB"
                     /locus_tag="JN691_002880"
     CDS             complement(5351..7372)
                     /gene="uvrB"
                     /locus_tag="JN691_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HAY7244284.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7951..8628)
                     /gene="bioD"
                     /locus_tag="JN691_002881"
     CDS             complement(7951..8628)
                     /gene="bioD"
                     /locus_tag="JN691_002881"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY7244285.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTV
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGELPWLAENPENAATGK
                     YINLALL"
     gene            complement(8621..9376)
                     /gene="bioC"
                     /locus_tag="JN691_002882"
     CDS             complement(8621..9376)
                     /gene="bioC"
                     /locus_tag="JN691_002882"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAY7244286.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9363..10517)
                     /gene="bioF"
                     /locus_tag="JN691_002883"
     CDS             complement(9363..10517)
                     /gene="bioF"
                     /locus_tag="JN691_002883"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAY7244287.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10514..11554)
                     /gene="bioB"
                     /locus_tag="JN691_002884"
     CDS             complement(10514..11554)
                     /gene="bioB"
                     /locus_tag="JN691_002884"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAY7244288.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11641..12930
                     /gene="bioA"
                     /locus_tag="JN691_002885"
     CDS             11641..12930
                     /gene="bioA"
                     /locus_tag="JN691_002885"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAY7244289.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGTMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12989..13465
                     /locus_tag="JN691_002886"
     CDS             12989..13465
                     /locus_tag="JN691_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAY7244290.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            13780..13849
                     /locus_tag="JN691_002887"
                     /pseudo
     CDS             13780..13849
                     /locus_tag="JN691_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         3231 a         3737 c         3537 g         3344 t
ORIGIN      
        1 tgcggttgcc accaaaccta aagcggtaat gagccatata ccgtttaata tcctggcctc
       61 gtgctgaaaa tctaatttat tgctgttttg cgagtatgat tccattcttt tagttgtcct
      121 tagaaaaaat attaatattc accatcaata aatttaaaac cacgcataca aaatactttt
      181 gtatttatgg atacggaaaa cgattccgct cgctcataag gcatatttta tacctggtac
      241 ttgttaagtg tcgtttttag taagaaaaaa atcccgacac atggccggga tagtgatggt
      301 gaaaaagaat taacggcggt tgccgaagat ccgtaacaac atcaggaaca ggttgatgaa
      361 gtccagatac aaggttaacg cgccaagaat ggaatatttg cgcaggttcg acgtgtcgcg
      421 ggtatcaatc tgctcaccca tatttttcaa tttctgagtg tcatacgccg tcaatccgac
      481 aaagacaatc acgccgatgt aggtaactgc ccacatcaat gcttcgcttt tcagccagaa
      541 gttgaccagc gatgccagca caatgccgat taacgccata aacagcatat tgccaaagcc
      601 acttaaatcg cgcttcgtgg tgtaaccgta caggctcatt gcgccgaaca tcccggcagt
      661 aacgacgaaa gtactggcga tagaagcagc ggtatagaca atgaatatac tggaaagcgt
      721 aagacccgtc agcgccgaat aaagcataaa gagcatcgtc gttacacctg cgctcagctt
      781 ttgaatcatc gctgataaca caataaccaa tgctaattgc gcgatgatca gaccgattaa
      841 aaagacacgg ttagtgaaca acagctccat cacggccgcg gaattagccg cataccaggc
      901 aacaaatgcg gtcagcaaca agccaacggt catccagcca tagacttgcg ccatataagt
      961 ttgcaagcca gcccggggtt gtacgattga atcagaacgt gggaatctgt ccatgacgat
     1021 ctcctgaaga tataaggaat atcttaagga tactgcaaaa tgatgaggct gtgcatcgac
     1081 gcagcgtaaa cgcatgtact gagcggtgaa attgccggac gcagcggtgc cttatccggc
     1141 taacaaaaaa ctaccagcgt tttgccgcct gcagatcgct ctcccgggct tcaacccagc
     1201 ggtcgccttc cggcgtggct tcgcgcttcc agaacggtgc gcgggttttg agataatcca
     1261 taataaactg cccggcttca aacgcactgc tgcgatgcgc actggtgaca ccgacaaaaa
     1321 cgatttcatc gcccggccat aattccccga tgcggtgaat caccgtgacg cgccccagcg
     1381 gccagcggtt acgcgcttca tcaacaattt ctgccagtgc tttttcagtc atccccggat
     1441 agtgttcgag ggttaatgct ttgacgctgt cgcccaggtt atggttgcgc acctttccgg
     1501 tgaaggtgac taccgcaccg tcttcgtcac gctccgccag ccacgggtac tcttctccta
     1561 cgctgaacgg ctgcgggccg acaacaattt tggtttctgc catcttaacc tccggttacc
     1621 ggcgggaaga aagctacttc gtcgccgtca gtcagcggat ggtcaaaact caccagcgtc
     1681 tggttgacgg cagccagcaa tttgccatct tccagcgcca gcgcccagcg atcgctctgc
     1741 gcagccaggt gctggcgtaa cgcttcaacg gttgggtaat ccgcagccac ttcggttgca
     1801 tctgttccca ccaactcgcg cacctgggcg aaaaaaagaa ctttaatcat ccgcttccac
     1861 cttaaagtca cccgacttgc cgccgctctt cgccagcaaa cgtaccggac caatcaccat
     1921 atctttttgc accgctttgc acatgtcata aatggtcagc gccgccacgg aggccgcggt
     1981 taacgcttcc atttcgacac cggttttccc ggtcaggcgg cataaggttt ctatacgcac
     2041 ccgattgtgc tccggctcgg cctgtaaatt gacttcaact ttgctgagca tcagcgggtg
     2101 acacagcggg atcagatccc aagtgcgttt tgccgcctga ataccggcaa tacgcgcagt
     2161 ggcaaatacg tcgcctttgt ggtggcgacc atcaataatc atcgccagcg tctcgctgcg
     2221 catggtgaca aaggcttccg cccgcgcttc acgcacggtt tccgctttgg cggagacatc
     2281 caccatgtgc gcttcgccag cggcgttgat atgggtcagt tgcgacatac ttatttcttc
     2341 aaatgtggat ggaaattaca cggacgcgta cgggcatcca gctgcggcgc gatgatattt
     2401 tcccatgcgg tacggcacgc tttggtcgaa cccggcatgg cgaaaatcag cgttttgtta
     2461 gcgacgcccg ctaccgcacg agattgcaac gtggaagtgc caatctcttc aaacgacaac
     2521 atacggaaca cttcaccaaa accttcaaac tcacggtcga acaacggcag cagcgcttcg
     2581 ggagcctgat caccttccgt caggccagta ccacccgtaa tcaataccac ttgtacatcg
     2641 tcgctggcga tccacgcaga tacctgagcg cgaatagcgt agcggttttc tttcacaatg
     2701 gctttatcga caacgtgatg gcccgcttct tgcgccgaat cgcgcagata gtgaccggag
     2761 gtatcgtctt cttcaccgcg acgattagaa accgtaagaa tagcaatacg ggtcgggata
     2821 aattcagtgc ttacctgact catctgatct ctccttttga cgttttagcc gccaatgtac
     2881 gataagtttt gcgtaatacc ggtgttgttt tgatgcagga aatgggtctg tttcttctcc
     2941 cgcagcgccg ctgaaatacg cgcttccagc gcctgttgct gggtatcgtc ttccagcaga
     3001 tcgcgcaggt taacgccgcc ttcaccaaac aggcagagat ggagtttacc aatggaggaa
     3061 acgcgcaggc ggttgcaagt ggcgcagaag tctttttcat acggcatgat aaggccaatc
     3121 tctccggcgt aatctggatg gcaaaagact tgcgcgggac cgtcgctgcg ttgacgtaat
     3181 tggtggatcc agccgcgacg cagtagctcg tcacgcagaa cctgaccaga gatgtgatgc
     3241 ttacggaaga gctcgctgcc ctcgcccgtt tccatcagtt cgatgaaacg cagctggata
     3301 gggcgatgct ggatccagtt cagaaaggtg tcgagctggt gatgattaac atcacgcatc
     3361 agcacggtat tgactttgac cttctcaaaa ccggcctcaa atgcagcatc aatccctgcc
     3421 atgacctggt tgaatttatc ctgcccggta atagcgtgaa actggcgggc gtccaaactg
     3481 tcgacactga cgttaatgcc agtaagtccc gcatcgcgcc agttcgccac atcgcgttcc
     3541 agacggtaac cattggtggt gaccgcaatc tggcggatag agtcgttttc ccgcacagcg
     3601 gcgatgatat cggtaaagtc gcggcgtaaa gacggctctc ctcctgtcag gcgcactttt
     3661 tcggtgccca gactggcgaa ggcgcgcgta acccggcgaa tttcatcgac ggtaagaaag
     3721 cctttattgg tgacgccgct cggtttgtag ccatccggca ggcagtaggt gcaacgaaag
     3781 ttacacacat cggtaatcga caggcgcaag tagtaaaact tacgcgcaaa tgcatcagtc
     3841 agttgtgaag ccatgtacac ctttccagat acgggaggcg aagtcatttc ttccttcgcc
     3901 ctggtggcaa tctttacgag cattgtcacg gccaaagcac cgtatcagtt gacccaggtg
     3961 cagaggctag agtgtttagt ggttaagccg atactagcgt gtaaatgaga tttttaccat
     4021 ccacactttc gctatgtaat catgtatata gcgtcatgat cgtgctattt cgctacagaa
     4081 taagggaaaa tggcggtttt actttgatat acatcatcag ggaggcatag cgtgcatcgt
     4141 cctttagaga tagaagaaaa tagtttgtat cctcaatatt ggcaggttaa ttgctgtttc
     4201 cccgcaattt gcgctactgt agcgcggata atctgactcc aggaaactat atgcgcaatc
     4261 gtacgctggc tgatcttgat cgtgtcgttg ctctcggcgg agggcatgga ctgggacgcg
     4321 ttctctcatc actttcgtct ttgggttctc gtttaacggg tatcgtcacc accacggata
     4381 atggtggctc gacggggcgt attcgccgtt cagaaggcgg cattgcttgg ggcgatatgc
     4441 gcaactgcct caaccagctg ataacggaac cgagcgtcgc ctccacgatg tttgaatacc
     4501 gttttggcgg caatggcgaa ctttccggtc ataatctcgg aaacttgatg ttaaaggcgc
     4561 tggatcacct tagcgtgcgg cctctggaag ccatcaattt aattcgtaat ctgctgaaag
     4621 tggatacgca tttgattcca atgtcagagc atcctgttga tctgatggcg attgacgatc
     4681 aggggcatga agtttacggc gaggtcaata tcgaccagtt aactacgccg attcaagagt
     4741 tattgttaac gcctaatgta cccgcaacgc gtgaggcggt tcacgctatc aatgaagcgg
     4801 atctcatcat tattgggcct ggcagttttt ataccagcct gatgccaatt ctgctgctga
     4861 aggaaatcgc ccaggcatta cgccgcacgc cagcgccgat ggtttatatc ggcaatctgg
     4921 ggcgtgagtt gagtttacct gcggctaatt tgaagctgga aagcaagctg gcaattatgg
     4981 agcagtatgt tggtaaaaag gtcattgatg cggtcatcgt cgggccaaaa gtggatgtct
     5041 cggcggtgaa agagcggatt gtgatccagg aggtactgga ggccagcgat attccgtatc
     5101 gtcatgaccg ccagttgtta cataacgcgc tggaaaaggc gttacaggct ttaggttaac
     5161 ggaaatccga aggaaaattc cggcttccta ttgaagacaa agtgcgcgtt gtttatgccg
     5221 gatgcggcgt gaacgcctta tccggcctac aaaccgcgca aattcaatat attgcggaga
     5281 aaatgtaggc ctgataagcg tagcgcatca ggctgttttc cgtttgtcat cagtcttctt
     5341 cgctatcctg ttacgatgcg gcaataaaca actcacgcaa ctgatgcaac tggtcacgaa
     5401 tttgtgccgc ttcttcgaac tccagattct gcgcgtgttg catcatcagc ccttccagtt
     5461 catggatttt ctgctgcaac gctttcggcg acatatccat cggcacatta tccggctcaa
     5521 caatcgggcg cgattttcct ctgcccttcg ctttggtttt ggcaatgttc tgccccagcg
     5581 ccaggatatc gaccactttc ttgttcaagc cttgcggcgt aatgccgtgt tcctcgttgt
     5641 acttctgctg tttctcacgg cgacgttcgg tttcgccaat cgctttcgcc attgatggag
     5701 tgatcttatc gccgtagaga atcgctttac cgttaacgtt acgtgccgca cgaccaatgg
     5761 tctggatcaa cgaacgttcg gaacgcagga agccttcttt gtcagcgtcg aggatcgcca
     5821 ccagcgacac ttccggcata tccagacctt cacgcagtaa gttgatccct accaatacgt
     5881 cgaactcacc cagacgcaag tcgcggataa tctccatacg ctcgacggtg tcgatatctg
     5941 agtgaagata acgcacgcgc tcaccgtgtt cttcgagata ttcggtaaga tcttccgcca
     6001 ttcgcttggt cagtgtggtg accagcacgc gttcgttaat tgccgctcgc tgacgaatct
     6061 ccgaaagaag atcatcaacc tgtgtcgcca ccggccgcac ttcgataatc gggtcaagca
     6121 atccggttgg acgcaccacc tgatccacca catcgccgcc ggatttttcc agctcgtaat
     6181 tacccggcgt cgccgaaaca tagatggttt gcggcgctaa tgcttcgaac tcttcaaact
     6241 taagcggacg gttatccagc gctgatggca ggcggaagcc gtactccacc agtgtctctt
     6301 tacgcgcccg gtcaccgcga tacatgccgc caatttgtgg aatggtgacg tgagattcat
     6361 cgacgaccag cagcccatcg gcaggcaggt aatcaaacag cgtcggcggt ggctcacccg
     6421 gtccacgacc ggagaggaag cgcgagtagt tttcaatccc cgaacagtag cccagctcgt
     6481 tcatcatctc cagatcaaac tgggtacgct gggtcagccg ctgctcttcc agcagtttgt
     6541 tgttttccaa cagcactttg cgtctggcgg ccagttcttc tttgatctcc tccatcgcct
     6601 gtacgatgcg ctcgcgcggt gtgacgtagt gcgttttcgg gtagatggta aaacgtggaa
     6661 tagtggaaac aatctgcccg gtcagcgggt caaataacga caatcgttcc acttcctcgt
     6721 caaacagttc cacgcgaagt gcaatgtcat ccgattctgc cgggaagata tctatcacct
     6781 cgccacgaac gcggaaagta ccacgctgga atgcttgatc attacgagcg tattgcagct
     6841 ccgccagccg gcgcagaatc gcgcgctgat cgataatcat accgaccgtg agatggagca
     6901 tcatcttgag atataaatca ggatcgccca gaccataaat cgcggaaaca gacgccacta
     6961 caatcacatc acgccgctcc agcatcgctt tggtggcgga caaacgcatc tgctcaatat
     7021 gttcgttaac cgaggcatct ttctcaatga aggtgtcgga actcggtaca taggcttccg
     7081 gctgatagta gtcgtagtag gagacgaaat actccaccgc gttttccggg aagaactctt
     7141 tcatttcgcc atacagctgg gccgccagcg ttttgttggg cgcaagtacc atggttgggc
     7201 gctgaaggtc agcaatgaca ttggcaatgg tgaaggtttt cccggagcca gtcacgccaa
     7261 gtaacgtctg gtgcgccagg ccatcttcca gcccctcttc gagacgtcga atcgcctctg
     7321 gctgatcgcc agaaggttta aaagcggaat tcagtttgaa cggtttactc atgagtcgct
     7381 acctgaagga gttgggcggg caggtatgta attttactcg tcgtacttaa ttatgccaac
     7441 aaattatact ggataaaaaa acagttcatc accataatat ttctgataca gcgtaaactc
     7501 cgctgtcaat catgagcaaa atttactctg tggcgagata aaactccggc cttaccgggt
     7561 tatccccaaa gcaacggctt ttttaacatt tgtcaagatg agtgatgaca gttttatggc
     7621 aagagatgcc tgttcagtga ctgccattgc atttatctaa ccagttaaaa aataaaagat
     7681 atttctttga gccgtcttta acgccaattc gcgtgcaagc cgcgtattct ctcgcttgcc
     7741 tcgtgttttc taactctaat acacatggtt atccacagga atagtggata actgtctcca
     7801 gcccctatcc cccgccgcct gggcaattcc cacattcgcc gcatgaggcg gaagtaaaat
     7861 ttttttcgtg attattttag aatttaattg gttaaattgc agtcaatcga agacgcgatc
     7921 tcgctcgcaa tttaaccaaa tacagaatgg ctacaacaag gcaaggttta tgtactttcc
     7981 ggttgccgca ttttctggat tttctgcaag ccaggggagc tctcccagca gcggcgcggg
     8041 aatcatgcgg gtgagcgtgg tcatatattc agcgtgacgt tttcccggag gcgtaacatc
     8101 gttcgccacc caacccgcca gagtcagtcc ggcgtgttgt attgcctgca cagtcaacat
     8161 cgcgtgatta atacagccga gtttcacacc aactaccagt atcaccggca gttgttcctg
     8221 tgttacccaa tctgcaaaag tgaaagtgtc agaaagcggc gtaaaccagc cgccagcacc
     8281 ttccactaac acccagtcag cctgttgttc aagcgcgcgt aatccggcgc tcattaccaa
     8341 tgattctatc ggcctgccct cttgggcgct gatgatgtgc ggcgaggtgg gttctgcgaa
     8401 ggtgtaagga tttaccgttg cgtaatccag ctgcaggctg ctgttgtgct gtaacgccag
     8461 cgcgtcacta ttacgtaagc cttccggggt cttttcgctg ccagaggcga ccggtttata
     8521 acctgccgtc cgataaccta ctgccttcgc ggcttgtaaa agtgcacaac tggcgacggt
     8581 tttccccact tcggtatccg ttccggtgac aaaataacgt ttactcacga gcaatcactc
     8641 ccaaaaaaag atgatacgtc agaggatatc gcccctgttg ttgcggccag gccagttgca
     8701 atcgctgcaa ctgcgaacgc gttaatattc gcgggtcgcg cccttcatga agatgcgtag
     8761 caccgatgcc tttcagcgaa cgcatggcac tgagcgcatc atcaaaccat agtgtgatga
     8821 gctgaatatg atgttgataa cgcacgccgt tcagcgactc ttcgatttca tctggcggta
     8881 aaaagcgatt agcatgcgga cgctcgtcca ccgcctgccg cgcctgatgc agttcgggta
     8941 acgatccctg caccagcgtg gtaaacgcga ccacgcctcc ggggcgcacc acccgataca
     9001 gctcgcggag tgccgtggat aaattaccgc accactgcac cgcgagattg ctccatgcaa
     9061 gatcgaacgt cgcagtcgct aacggcaggg attcgatatc tcccgccaga taatgatctg
     9121 cggcatcttt atggcgggct tgcgcaagca ttggcggcga gagatctaac gccgtcacct
     9181 gcgcgtgacg ttcccgccag tggcggctca tccagccagg tccacaaccc gcgtccagta
     9241 cgtgggtgta tttacgctgt ggaagcattg ccagtaaggc gtcagcactc tggcgctgta
     9301 gatctgcatg ttgctcatag tgtgcggctg cccgaccaaa tgccgctgca atggcttgtt
     9361 tattaaccgt tgccatgcag cacctccagc agacggtcga tatcctgcat ttcatgcgca
     9421 gcggttagcg ttaagcgcag tcgcgcagta ccagcgggta cggttggcgg gcgaatcgcc
     9481 gtgacccagc agccttgctg gcgcagtttt tctgccagtt gtaacgcacg gctgttatca
     9541 ccgacgatca atggctggat ggcgctgcat gaatcagcca gcgtaaacgg caaatcctgt
     9601 actccggcac gaaaacgcgc aatgagtgac accagttttt cgcgccgtgc atccccctca
     9661 tcacggcgaa tgaccgccag cgacgcacgt aatgcctgcg cctgagcggg cggcatactg
     9721 gtgctgtaga taaggtggcg ggcgaattgc agcagataat ccgccaccgt acttgagcaa
     9781 agcaccgcag ccccactgac gccaaatcct ttgccaaaag tcactaccag caattctggt
     9841 tttacctttt gcagccagca gctgccgcgc ccctgctccc cgataacgcc cgtgccgtgg
     9901 gcatcatcga ccatcaacca gccattgtgc tgttgcgtta cctgctggat ttccgccagt
     9961 ggcgcgctat cgccgtccat gctgaacacg ccttctgtca ccaccagttg ctgccccgga
    10021 cagggggaag caagcagtcg cgccagatga gcgacatcgt tatgagtaaa acggcgaagc
    10081 tgcgacgggc ttaaactggc agcctccagc aatgaggcat ggctaagccg gtcggcaaca
    10141 atacggtcct ctttcgccat catcgcggca atgactgcct gattcgcggc aaaaccagag
    10201 ataaacagca gcgcccgcga atagccaagc cactcggcca attcttcttc cagcgcctga
    10261 tgcaccacgc tataaccgct gacgtgaccg gagccgccgc taccgatgcc aaattgctcc
    10321 gccccctgct tccaggcacg gataatttgc ggatgatggc ttaaacctaa ataatcgtta
    10381 ctggaaaagt tcagatactg gcgatcatcc gccaccagcc agcgtccggc tccttgcgcc
    10441 accggataac ggcgacgcag ggcatcggca gcacgccgcg catcgagcgc cgcgttgatt
    10501 ttctcctgcc agctcataat gctgccgcgt tgtaatattc gtcggtgtcc ggggtcatca
    10561 gcgcctgttc aagacgttgc tgttgttcgt tatcccctgc cagcacggca gtttgctgcg
    10621 gatttagccc cagtttgcgg aacagttgca ggtctttatc ttcttccgga ttcggcgtgg
    10681 tcagcagttt gcaaccgtag aaaatcgagt ttgcgcctgc cataaagcac atcgcctgag
    10741 tctgttcgtt catctgctcg cgtccggcag aaaggcgcac gtaagaggtc ggcatcatga
    10801 tccgcgctac ggcaatggtg cgaataaaat caaaggcatc aacgtcatcg ttatcggcaa
    10861 gcggcgtgcc tttcaccttc accagcatgt tgattggtac gctttccggc ggcgtcggca
    10921 ggtttgccag ttgcagcaat aatccggcgc gatcttttac cgtttcgcct aagcccacaa
    10981 tgccacctga gcagactttg atcccggcat cgcgcacttt ttccagcgta tcgaggcgtt
    11041 cctgataagt gcgtgtggtg atgatattgc cgtaaaactc cggcgaggtg tccaggttgt
    11101 ggttgtagta atccagcccg gcgttcgcga ggcgctgcgc ctgagattca ctcaacgtgc
    11161 ccagcgtcat acacgcctcc agccccatcg cttttacccc ctgcaccatt tgttccaggt
    11221 acggcatatc gcgttcgtgg ggattcttcc acgccgcgcc catacagaag cgcgtcgatc
    11281 ctgccgcttt cgctttgcgc gccgactcca gcacctgttc aacttccatc aaccgctcgg
    11341 cttccagccc ggttttgtag cgcgagcttt gcgggcagta tttgcaatct tccggacaag
    11401 ctccggtctt aatcgacagc aacgtgctga cctgcacctg acgaggatcg aaatgctggc
    11461 gatgcacctg ctgcgcttca aacagcagat ccagcaacgg tttttcaaat aattctgtga
    11521 cttgcgacaa tgtccagcgt gggcggtgag ccatggggct tctccaaaac gtgttttttg
    11581 ttgttaattc ggtgtagact tgtaaaccta aatcttttca atttggttta caagtcgatt
    11641 atgacaacgg acgatcttgc ctttgaccaa cgccatatct ggcacccata cacatccatg
    11701 acctcccctc tgccggttta tccggtggcg agcgccgaag gttgcgagct aattttgtct
    11761 gacggcagac gcctggttga cggtatgtcg tcctggtggg cggcgatcca cggctacaat
    11821 cacccgcagc ttaatgcggc gatgaagacg caaattgatg ccatgtcgca tgtgatgttt
    11881 ggcggtatca cccatgcgcc cgccatcgag ctatgccgca aactggtggc aatgacgccg
    11941 caaccgctgg agtgcgtttt tctcgcggac tccggttccg tagcggtgga agtggcgatg
    12001 aaaatggcgt tgcagtactg gcaagccaaa ggcgaagcgc gccagcgttt tctgaccttc
    12061 cgcaatggtt atcatggcga tacctttggc acgatgtcgg tgtgcgatcc ggacaactca
    12121 atgcacagcc tgtggaaagg ctatctgcca gaaaacttgt ttgctcccgc cccgcaaagc
    12181 cgcatggatg gtgaatggga tgaacgcgat atggttggtt ttgcccgcct gatggcggcg
    12241 catcgtcatg aaatcgcagc ggtgatcatt gagccgattg tccagggcgc aggcgggatg
    12301 cgcatgtacc atccggaatg gttaaaacga atccgcaaaa tgtgcgatcg cgaaggtatc
    12361 ttgctgattg ccgacgagat cgccaccgga tttggtcgta ccggcaaact gtttgcctgt
    12421 gaatatgcag aaatcgcgcc ggacattttg tgcctcggta aagccttaac cggcggcaca
    12481 atgacccttt ccgccacact taccacgcgc gaggttgcag aaaccatcag taacggcgaa
    12541 gccggctgct ttatgcatgg gccaactttt atgggcaatc cgctggcctg cgcggcagca
    12601 aacgccagcc tggcgattat cgaatccggc gaatggcagc agcaggtggc ggctattgaa
    12661 gtgcagctgc gcgagcaact ggcaccagcc cgtgatgccg aaatggttgc cgatgtgcgc
    12721 gtactggggg caatcggtgt ggtcgaaacc actcgtccgg tgaatatggc ggcgctgcaa
    12781 aaattctttg tcgaacaggg ggtatggatc cggccttttg gcaaactgat ttacctgatg
    12841 ccgccctata ttattctccc gcaacagttg cagcgtctga ccgcagcggt taaccgcgcg
    12901 gtacaggatg aaacattttt ttgccaataa cgggaagtca gcgtgagggt ttctggctac
    12961 actttctgca aacaagaaag gagggttcat gaaactcatc agtaacgatc tgcgcgatgg
    13021 cgataagttg ccgcatcgtc atgtctttaa cggcatgggt tacgatggcg ataatatttc
    13081 accgcatctg gcgtgggatg atgttcctgc gggaacgaaa agttttgttg tcacctgcta
    13141 cgacccggat gcgccaaccg gctccggctg gtggcactgg gtagttgtta atttacccgc
    13201 tgatacccgc gtattaccgc aagggtttgg ctctggtctg gtagcaatgc cagacggcgt
    13261 tttgcagacg cgtaccgatt ttggtaaaac cggatacgat ggcgcagcgc cgccgaaagg
    13321 cgaaacccat cgctacattt ttaccgttca cgcgctggat gtagagcgta ttgatgtcga
    13381 tgaaggagcc agcggcgcga tggtcgggtt taacgttcat ttccactctc tggcaagcgc
    13441 atcgattacc gcgatgttta gttaatcact ctgccagatg gcgcaatgcc atctggtatc
    13501 acttaaaggt attaaaaaca actttttgtc tttttacctt cccgtttcgc tcaagttagt
    13561 ataaaaaagc tgaacgagaa acagtaaaaa tcattaatat caatgcatta taatacattt
    13621 agcttaaaaa atagactgca taatactgca aaacacaaca tatccagtca ctatgaatca
    13681 accacttaga tggtattagt gacctgatac agagcattag cgcaaggtga ttttttgtcc
    13741 tcttgcgcta attttttgtc aacgcactgg gttagctgaa tttacaacca tgctcagtat
    13801 ctcgataagc gcagagaaat gatgctgtgg tgggcagact actggcttg
//
LOCUS       DADLSA010000099        13753 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.099, whole genome shotgun sequence.
ACCESSION   DADLSA010000099 DADLSA010000000
VERSION     DADLSA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.099"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(19..765)
                     /gene="dsbG"
                     /locus_tag="JN691_002888"
     CDS             complement(19..765)
                     /gene="dsbG"
                     /locus_tag="JN691_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAY7244291.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            1137..1700
                     /gene="ahpC"
                     /locus_tag="JN691_002889"
     CDS             1137..1700
                     /gene="ahpC"
                     /locus_tag="JN691_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAY7244292.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1945..3510
                     /gene="ahpF"
                     /locus_tag="JN691_002890"
     CDS             1945..3510
                     /gene="ahpF"
                     /locus_tag="JN691_002890"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAY7244293.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRASGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3631..4059)
                     /gene="uspG"
                     /locus_tag="JN691_002891"
     CDS             complement(3631..4059)
                     /gene="uspG"
                     /locus_tag="JN691_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAY7244294.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            4280..5518
                     /locus_tag="JN691_002892"
     CDS             4280..5518
                     /locus_tag="JN691_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HAY7244295.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5749..6159)
                     /gene="rnk"
                     /locus_tag="JN691_002893"
     CDS             complement(5749..6159)
                     /gene="rnk"
                     /locus_tag="JN691_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAY7244296.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6388..7194)
                     /gene="rna"
                     /locus_tag="JN691_002894"
     CDS             complement(6388..7194)
                     /gene="rna"
                     /locus_tag="JN691_002894"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAY7244297.1"
                     /translation="MKAFWRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            complement(7308..8771)
                     /gene="citT"
                     /locus_tag="JN691_002895"
     CDS             complement(7308..8771)
                     /gene="citT"
                     /locus_tag="JN691_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HAY7244298.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIIGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8822..9700)
                     /gene="citG"
                     /locus_tag="JN691_002896"
     CDS             complement(8822..9700)
                     /gene="citG"
                     /locus_tag="JN691_002896"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAY7244299.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTIVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9675..10226)
                     /gene="citX"
                     /locus_tag="JN691_002897"
     CDS             complement(9675..10226)
                     /gene="citX"
                     /locus_tag="JN691_002897"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAY7244300.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRIEALLNDVDACNVN"
     gene            complement(10230..11761)
                     /gene="citF"
                     /locus_tag="JN691_002898"
                     /pseudo
     CDS             complement(10230..11761)
                     /gene="citF"
                     /locus_tag="JN691_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
     gene            complement(11772..12680)
                     /gene="citE"
                     /locus_tag="JN691_002899"
     CDS             complement(11772..12680)
                     /gene="citE"
                     /locus_tag="JN691_002899"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAY7244301.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12677..12973)
                     /gene="citD"
                     /locus_tag="JN691_002900"
     CDS             complement(12677..12973)
                     /gene="citD"
                     /locus_tag="JN691_002900"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAY7244302.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12988..>13753)
                     /gene="citC"
                     /locus_tag="JN691_002901"
     CDS             complement(12988..>13753)
                     /gene="citC"
                     /locus_tag="JN691_002901"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAY7244303.1"
                     /translation="TEYEALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRH
                     PGNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGT
                     ADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRF
                     VGTEPFCRVTAQYNQDMRYWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKN
                     DLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3368 a         3496 c         3660 g         3229 t
ORIGIN      
        1 ccatccatga attacctttt atttatttcc cataatgata ttaagcgttt tctgatcggg
       61 caaccccacg gcctgttgca gcgtattttc cttactcatg taatagatag ccggcgtgac
      121 atttgccccc agatcgtcca tcagtttctc attgtcactt aacactttca tttgctctgt
      181 acttacgttt gcaggcacgt ttagcttaag cttgcaacca gaggcttcat attgttgcca
      241 ggtttttgcg ggatctttgg aggcaagaat tgccgctgct gtcgccgggc tttctggctt
      301 gataacccca accaacaatg ttcttaattg cactttgcca gaatctaccc acgggcgcgc
      361 ctgctgccag aactgtttac aatatgggca gaacggatcg gcgaagacgt agacaatgac
      421 cggcgcatct tttttaccgt cgaggagcca gtgggattgt tccatccgtt gccacatttc
      481 gcgtccggct ggtgcgtaaa tttctttttc gataagtgtg ttactcaggt tttcaccttt
      541 ctcgttgtac atgtaaccag agatagcgtg cttaccatcg ggagtcaggt agatggtgac
      601 gcccatatcc tgatactttc cgagataacc tttcattcct ccgggggcat cgaacgtttt
      661 gatgattgta atgccctgtt tttcaatcgc ttttactggg gaaggaagtt cctctgcgaa
      721 ggcgattgca ggaagcagag ccagtaaaag tatctttttt aacattaatt tgtccttttc
      781 agtcagtgca aaagtcgagt aaaaggcata acctatcact gtcataggta agagcttaga
      841 tcaggtgatt gccctttgtt tatgagggtg ttgtaatcca tgtcgttgtt gcatttgtaa
      901 gggcaacacc tcagcctgca ggcaggcgct gaagatacca aagggtagtt cagattacac
      961 ggtcacctgg aaagggggcc attttacttt ttatcgccgc tggcggtgca aagttcacaa
     1021 agttgtctta cgaaggttgt aaggtaaaac ttatcgattt gataatggaa acgcattagc
     1081 cgaatcggca aaaattggtt accttacatc tcatcgaaaa cacggaggaa gtatagatgt
     1141 ccttgattaa caccaaaatt aaacctttta aaaaccaggc attcaaaaac ggcgaattca
     1201 tcgaaatcac cgaaaaagat accgaaggcc gctggagcgt cttcttcttc tacccggctg
     1261 actttacttt cgtatgcccg accgaactgg gtgacgttgc tgaccactac gaagaactgc
     1321 agaaactggg cgtagacgta tacgcagtat ctaccgatac tcacttcacc cacaaagcat
     1381 ggcacagcag ctctgaaacc atcgctaaaa tcaaatatgc gatgatcggc gacccgactg
     1441 gcgccctgac ccgtaacttc gacaacatgc gtgaagatga aggtctggct gaccgtgcga
     1501 ccttcgttgt tgacccgcag ggtatcatcc aggcaatcga agttaccgct gaaggcattg
     1561 gccgtgacgc gtctgatctg ctgcgtaaaa tcaaagcagc tcagtacgta gcttctcacc
     1621 caggtgaagt ttgcccggct aaatggaaag aaggtgaagc aactctggct ccgtctctgg
     1681 acctggttgg taaaatctaa atttccttcg tctttcacgc catagcggcg ttggcgtcgc
     1741 ccgctcaccc cggtcactta cttgtgtaag ctcccgggga ttcacaggct agtcgccttg
     1801 ctctgacgcg aaatacttcg gaaattcacc taattcttcg ggtgctgcgg cacccgattt
     1861 tcttccccgc accatgatgc aagctgcatc caggtagccg cagaggccgc ttgcatgatg
     1921 atgtttaaag cccaggagat aaacatgctc gacacaaata tgaaaactca actcaaggct
     1981 taccttgaga aattgaccaa gcctgttgag ttaattgcca cgctggatga cagcgctaaa
     2041 tcggcagaaa tcaaggaact gttggctgaa atcgcagaac tgtcagacaa agtcaccttt
     2101 aaagaagata acagcttgcc agtgcgtaag ccgtctttcc tgatcaccaa cccaggttcc
     2161 aaccagggac cacgttttgc aggctccccg ctgggccacg agttcacctc gctggtactg
     2221 gcgttgctgt ggaccggtgg tcatccgtcg aaagaagcgc agtctctgct ggagcagatt
     2281 cgccatattg acggtgattt tgaattcgaa acctattact cgctctcttg ccataactgc
     2341 ccggacgtgg tgcaggcgct gaacctgatg agcgtattga acccgcgcat caagcacact
     2401 gcaattgacg gcggcacctt ccagaacgaa atcaccgatc gcaacgtgat gggcgttccg
     2461 gcagtgttcg taaacgggaa agagtttggt cagggccgca tgacgttgac tgaaatcgtt
     2521 gccaaaattg atactggcgc ggaaaaacgt gcggcagaag agctgaacaa gcgtgatgct
     2581 tatgacgtat taatcgttgg ttccggcccg gcgggtgcag cggcagcaat ttactccgca
     2641 cgtaaaggca tccgtaccgg tctgatgggc gaacgttttg gtggtcagat cctcgatacc
     2701 gttgatatcg aaaactacat ttctgtaccg aagactgaag ggcagaagtt ggcaggcgca
     2761 ctgaaagttc acgttgatga atacgacgtt gatgtgatcg acagccagag cgccagcaaa
     2821 ctgatcccgg cagcggttga aggtggtcta caccagattg aaacagcttc tggcgcggta
     2881 ctgaaagcac gcagcattat cgtggcgacg ggtgcaaaat ggcgcaacat gaacgttccg
     2941 ggcgaagatc agtatcgcac taaaggcgtg acctactgcc cgcactgcga cggcccgctg
     3001 tttaaaggca aacgcgtagc ggttatcggc ggcggtaact ccggcgtgga agcggcaatc
     3061 gacctggcgg gtatcgttga gcacgtaacg ctgctggaat ttgcgccaga aatgaaagcc
     3121 gaccaggttc tgcaggacaa actgcgcagc ctgaaaaacg tcgacattat tctgaatgcg
     3181 caaaccacgg aagtgaaagg cgacggcagc aaagtcgttg gtctggaata tcgagatcgt
     3241 gccagcggcg atattcacaa catcgaactg gccggtattt ttgtccagat tggtctgctg
     3301 ccgaacacca actggctgga aggcgcagtc gaacgtaacc gcatgggcga gattatcatt
     3361 gatgcgaaat gtgaaaccaa cgtcaaaggc gtgttcgcag cgggtgactg tacgacggtt
     3421 ccgtacaagc agatcatcat cgccactggc gaaggtgcca aagcctctct gagtgctttt
     3481 gactacctga ttcgcaccaa aactgcataa gaagaagtaa gattcacctg caattgctta
     3541 gccgccgggg tcaaacctgg cggctttttt atggcattaa aaagccctgc cgggatgttc
     3601 caggcagggc ggcataaata cttttatctg ttagcgaacg accagtacag gcagattggc
     3661 gtggcggatc acgctcgagg cgttagaacc taacagatgg gtcgaaatcg atgggttgcg
     3721 agaaccgata actacaacat cagcccccag ttcttctgcc aactcattga cttcatcccg
     3781 cacgctacca aaacggacat gttgtttaat gcgggaagga tcgatggtga agtggctgac
     3841 catcgtttgc agacgttctt ctgcttcatg ttgcagatgc tcttcaaaac gacgcacatc
     3901 agcggcaaaa cggtgcaggc tcaggctggc tgacccgggt agtacgtgaa gtagatgaat
     3961 aactccgtca tcctgggcga ggaattcagc gtggcgaaca gctttgtcgc tcaattccat
     4021 ttcaaataca tcaactggca taatgattgt cttatacata accctttctc cctgttaatc
     4081 atgaacaaat cattcgccat gattataata tttatccctg atatttgtct ggttcttttt
     4141 ccttacgaac ggtttctgtg attaatatat tctcactgaa caccaggaat tctcccaaaa
     4201 cctgcggtac cgcccgtttt cccgctgtga tagctaccct taaagactga ctcttttttg
     4261 aactgtctct ggaggttgca tgaaagcatt gacttatcac ggcccacatc acgttcaggt
     4321 agaaaatgtt cccgatccgg gcattgaaca ggcagatgat attattctgc gtattacggt
     4381 aacggcgatc tgtggctctg acctccatct ttatcgaggc aaaatacccc aggttaaaca
     4441 tggcgatatt tttggtcatg aatttatggg ggaagtcgtt gaaaccggga aggacgtaaa
     4501 aaatttgcaa aaaggcgacc gggtggtaat tccgttcgtc attgcttgtg gcgactgttt
     4561 tttctgtcga ttacagcaat atgccgcctg cgaaaatacc aatgcgggta aaggcgctgc
     4621 gctcaataaa aaacagatac ctgctcccgc ggcattgttt ggttatagtc acctgtatgg
     4681 tggcgttcct ggcgggcagg cggaatatgt ccgcgtccct aaagggaatg tggggccgtt
     4741 taaagtaccg cctttgcttt cagatgataa agcccttttc ctttctgata ttctgccaac
     4801 ggcatggcag gcagcaaaaa atgtgcagat ccaacaaggt tcaagcgttg cagtctatgg
     4861 ggctggtcct gtgggattgt tgacaatcgc ctgtgcacgg ttgctcggtg cggaacagat
     4921 ttttgttgtt gatcatcatc cctaccgctt gcgtttcgcc gctgaccgct acggcgcgat
     4981 cccgattaat ttcgatgaag atagtgatcc ggcacagtca attattgaac aaacggcagg
     5041 tcaccggggc gtggatgcag taatagacgc cgtcggtttt gaagcgaaag gcagcaccac
     5101 ggaaacggtg ctgactaacc tgaaactgga gggcagcagc ggtaaagcgt tgcgccagtg
     5161 tattgcggcg gtcaggcgtg gcggcattgt tagcgtaccg ggcgtctacg ctggatttat
     5221 tcacggtttc ctgtttggcg acgcctttga taaagggttg acgtttaaaa tgggacagac
     5281 ccacgttcac gcatggctgg gagaattatt accgttaatt gagaaaggat tactgaaacc
     5341 agaagaaatt gttacccact atatgccgtt tgaagaggcc gcccggggat atgagatttt
     5401 cgaaaaacgt gaagaggagt gccgtaaggt gattctggta cccggtgcac aaagcgcaga
     5461 ggcggcgcag aaggcggttt caggtctggt gaatgcgatg ccggggggaa caatatgatc
     5521 gtcaggagtg gttttcgagg taaaggacag ccatgacaat aatcgccgcc ataatcagaa
     5581 atcctatcag gatgtaaaat gcttctgcca tggttattcc cacaaacgaa acggaataat
     5641 tttgcagcaa aagtgaacag tgagaaccag gaaaaattgc tgattttgcg taaagaggat
     5701 gcgagtgcat cctctgggca aagcgagtta tcgcttgtgc agatgggatt aaagcaggta
     5761 gtcgccagca gcttctggct ggtattcgag ttccagcact tcaaggtggg ttgcaacgcc
     5821 gcccggaagt tcccagtgaa tggaatcgcc aacgcgcagc cccagcagtg cggcacctac
     5881 cggagccata acggaaagct gagtattgct atcggtcatt tttgccggat acaccagcgt
     5941 gcgcacacgc acttcgccat cgctaagatt gcggaattta acccggctgt tcattgtcac
     6001 cacgtcgtgt ggcatctctt ctggcgaaca catttgggcg cgatccaact ctgcgtttaa
     6061 cgcgtcggcg attggcaaac cagcataggc gggttgctcc agcagaatat cgatgcgttc
     6121 ggcatccagg tcgttaatga tgatagttgg tctggacatt tttactccat gtcgtcggta
     6181 ctgcgagtgt cgcagataaa catacccaaa agaaaaccct caccgtcagg cggcgagggt
     6241 ttaactcaca tgatgatact gactgttgct cactctttga agtgatttgc gtcacattca
     6301 gggaattcct caatgcagcg cattatgtat aattcttaat cggcatggtt atggaagacg
     6361 aattagcgtg ttttgtaaat cagaggatta ataacccgct ttatcaatca caaaggtttt
     6421 gccacagtta cctgggtgag gttgtggcaa gaatgagttt gcagaaagcg gagcgttgat
     6481 ggcgtcagct ttgatcgaga tctgaatttc agtcaaatac gcagggttac cctggcacgt
     6541 tagcttaact gctttcacgt tctcttttcc ccagcttttg gcaaaggcgg cgtcaaagtc
     6601 acggcggctc actgttttac cgtaattatc cgcaaggaat tttccggctt cgctctcttt
     6661 aatttcttga ttcaggcgta ccatcgtacc gaagtatgcg tccggatcaa aaccaaagca
     6721 ggcaccgtgt ttggcatatt cgtagcgttc caggcaggaa cgtccgccag ctcctggcat
     6781 gacttcactt agtttagcgg ccgtttccag tgataacccg gtttccggcg atgaacacat
     6841 tcggctggcg cgtgcttctg gtagattcgg gattgggcga gtagcgcaac cgaagcgcat
     6901 ccagcggcgt tcatcaacac cacgggcagc aaccgattta ggcaatcctg gccacagacc
     6961 atgtacggtc agaaaatcag ctttgttggt cgtttcggtt tgcaggcgac attcatctcg
     7021 ttcgttacga tttcgatcgt gttgactctg gcaaaatccg gtttgccagg agagggccag
     7081 gacatagcga tcaaaatcgc catactgttt tgcctgcaac gctacggcgt tggcagaaga
     7141 taagggaagc agagaaaccg cgagcaacgc ggcgttacgc cagaatgctt tcataatggg
     7201 tgtggaactc atacatacac tgaatactat ctattaaatc ataaaaaagc ccgccaggtg
     7261 ggcttacctg gcgggcgtga tgatttattc agcgtttggc gaacgtatta gttccacatg
     7321 gcgagaatcg gccagccaac caacagcaac atagagatgt aaatcacccc gaagattgcg
     7381 ccaagacgcc agtaatcttt tgatttcaca tagccacagc cgtaaataat caccccagga
     7441 ccggttgcat acggcgtcag acagcctatg ataccgatag acagcaccag caggatacac
     7501 agttgttcca ttggtacgcc cggaatacct ttaccgacgg ccagaataac cggcagcatg
     7561 gttgcggtgt gcgcagacag gctggcaaac aggtagtgtg caaagtagaa caccagaacc
     7621 agtacaatca ccgttgcgtt tggtgagaat ccttccaggt gcgtactcat ggtaccggcg
     7681 aaccagtcaa taaaaccaga acgagtcagg ccgttagcca tcacaaccag agttgccagg
     7741 ttgaccagcg tgttccatgc gctgttatag cgggtaatgt ctttccacgg cacaacgtgc
     7801 agagccagca ttagcgaaac tgccagcaga ccaaccgcag tagcattaat gacttcactg
     7861 ccaaataccc acaaacctaa gctgagcaat acaaggccaa tcagcgtcca ctctctgcgt
     7921 gtcagcgcac ccatggtttt tagttcatca cccgcccagg ttgccacttc ttcactgtgt
     7981 gtgatttccg gtttgtacag cacgtaggaa agccacggcg caatgataag caagataacc
     8041 ccaaccggca ggaagcagag gaaccactgc aaccagctaa tctggatacc ggcaattttg
     8101 ctgacgaact ccagacccag cacgtttggt gccgcaccgg tgacaaacat ggacgaactc
     8161 agactggtac taatgaccat catccacatc aaatagccgc caatacgacg cgcggacgga
     8221 tcgttcggga atgatttaaa caacggcggc aggtttttaa tgaccggaaa aaccgtaccc
     8281 ccggtacgcg cggtgttgga cggtgtaaac ggtgccagca gaatgtcgat aatgacaatc
     8341 gcataaccca acgtcagcgt gcgtttgccc atgaatttca ctaggaaaag ggcaatgcga
     8401 cgacctaacc cggaaacttc ataccctaat gcaaaaataa atgcgccaaa taccagccat
     8461 accgtggtgc tggaaaaacc agccaggccc catttcagcg cctgtttttg cgcattaaac
     8521 gctgggtcag ctaattcttt ggcatcaaag agcaggtaat tactgccaat aacgcaaata
     8581 gtaaccgcaa taaaactgat cgctgttgcc ggaattggct cgaggatcat gccgacaatc
     8641 attgccacaa acacagcgaa gtaatgccat gcctgcggcg gcataccgtc ggggacaggg
     8701 ataagaaaca tgacacccat caccaccagt ggggccaata gtttccatat attatctttt
     8761 gctaaagaca tacgggttct ccgaaaatta atatttccaa atttatcaag tgcttaaata
     8821 attaaatctg tgctaaaaac caggtaagga tcagcaggtc ggcactgccg cctggactga
     8881 gatttcgttc gatacactcc ctgtcgaact gccggagata atcgagatcg gcgggggttc
     8941 gaatgccccc tttttgcaat aatgtttgcg cctcgcgctg tagccagcgc aggcccccct
     9001 cgccaccgcg cgatgcaacg ttggtatcgc cgttggtagc catcagtagg agcaaggtat
     9061 cgagcaatgc cagttcagga tctaacccct gatccagcag agtgaggtaa tgcggcaagg
     9121 cgtggttgat caccagtgga taacccgctt cggcttcacc gcgtgcgccg gtaaggccaa
     9181 gctgctggta caaccgttga cctgccgtca gtcgtgaatt attggtacgc agttcgcgat
     9241 cggtcaggcc acggcagaaa cttgccgccg tagaacaaac gattgttggc gtaaccggct
     9301 ggttgagctg aagcaaacgg ccaattgccg cacatagcag ccctaaagaa aaaatgctgc
     9361 ctttatgcgt gtttacgccc gcagtggcgc ggaacatatc accttcgcaa gccataccaa
     9421 ttgggcgtaa tccgtggagt accgcttccg gtgccatttc tgcactacag gcaccaaatt
     9481 caatgaaacg gggtaaccag ccctgaatcg ccagcgcgct gcggtggaaa tcttccagcg
     9541 ccatatcttt gtgcgcaccg cagttaatgc gatccacgag gcctggtttc ggtgacagat
     9601 tgacttcagt cagcatggcg cgccagccca gcagggcgta ctcatcgatt aatgacgtcg
     9661 caagctttgt ggttttagtt gacgttgcag gcatcgacat cgttcagcag tgcctctatg
     9721 cggttgagta aatcggtcag ttgatgggtt tttccacgcg cgcagacggc tgcgctttgc
     9781 tcgcacaaca ggcagcggcg aggcggcagt gaatagtcgc ggcgggagag aatttcgcct
     9841 tcgggcgtca ggacatcgat atcccataac cgcccgagag gatgactatg ttcaagctca
     9901 atggtggcga gcttgaggtc gcgagccggg gcggcaatgc tcaacatgcc ctccggcccg
     9961 ctggcggaaa ccagtgcagc ctgctcctga atttgccagc cctgctttgt ggctaaggca
    10021 cgcaaggctg tcacgccatg attaaaaatt cggcgtgtga cctcgctgtc tttaatcggc
    10081 ccaggcgcaa ccacggtaaa ggagaccagt ggaacaggat ggtgcttgag ccaggcgtgt
    10141 tgccgtgctt gcctttcatc ccggctgacg agcagctcgg gaattgacac cgcatggtgg
    10201 ctggcgagtt caggaagcag gtgcatggct tattccttca cctgatgcac aacatcgatc
    10261 accgagccat cgcggtaacg cacaacggca acgacgcggt cggtgaattc aatcggctgt
    10321 ggttcaccgg tcagcagacg cgcacgttcg cgcagccact caatggaaac cactttaatg
    10381 cccgcttcct gcagacgttc tgctagttcc ggacgtgccg ggttaactgc gataccgtgg
    10441 tctgtgacca gaatatcgac actggagccc ggggtgatgc aggtcagcac gttatccacc
    10501 agagtcggaa tacgaccgcg taccagtggc gcgacgatga tggaaagcgc agaggcaatc
    10561 gcggtatcgc agtgaccacc ggaagcgcca cgcagtacgc cgtcagaacc ggtcagcacg
    10621 ttaacgttga actgcctgtc aatttccagc gcgctcagta ccaccacgtc gagacgatca
    10681 accgatgcgc ctttcgaccc cagttagcgt actggttggc gctgatttcg atgtgattgg
    10741 ggttacgggc cagcgattgc gcagcatggc tgtcaaagct ctgcacatcc agcagtttgc
    10801 ggatcagacc tttttcgtgc agatcaacca tcgtcgcggt aataccgccg agggcgaagt
    10861 cggcgcgaat atcgcggcta cgcattttgt cttccaggaa acgggttacc gccagcgaag
    10921 cgccgccggt gccggtttgc atggagaaac cttctttgaa gtagccagag ttgacaatca
    10981 catccgcagc gctacgggca ataagcagtt cgcgcgggtt agtggtcata cgggtagtac
    11041 cagcgccgat ttttgcagca tcgccaacgc gatcaacttt gacgatcaaa tcaacctgat
    11101 cttgctcaat gcttgccgga ttatgcggat aaggcagcag ttcttcggta agcatcacga
    11161 cctgttttgc gttgtcggca tcaaccattg catagccgag ggagccgcag caggctttac
    11221 cggtgtagcc gttggcatta ccgaattcat cacaggacgg gacgccgagg aaagccacgt
    11281 cgatattcag ttcgccgctc tgtaccagat gcacacgacc gccgtgagag tggatctgca
    11341 ccggttccgc cagcagacca cgggagatct cttccgccag tggaccgcgt aggccagagg
    11401 tataaatgcg ggtaaccacg ccctggcgaa tgtgttctac cagcggcaca tggcaatcac
    11461 tcagggagct ggacgccagg gtcaggtttt taaagcccat cttcgcgatg acgtccatca
    11521 ccatattgac ggtcaggtca ccgccacgga aagcgtgatg gaaggaaacc gtcatgccgt
    11581 cctgtaaacc tgagcgacga atcgcttctt ccaggttggc gcacagtttg cgatcgcgcg
    11641 ctttttcagc ctggtaggtt tgctttggtg agttctggaa agcggcaaga tcgcattcag
    11701 tgcgacgatt ccaggccgct acccgttctt gtcgttgaga ttgttcaatt ttctgcgtca
    11761 ttttgattgc cttattcttc gcggatgccg gaaagttctg cacgggagag caccagacgg
    11821 gcgcgatcga taaccggacc gtccaccatc ttgccgttca gggaaaccac gccgaggcct
    11881 tcgcgagcgg cggcttcagc ggcttctacg acgcggcggg cgtgatccac ttctttctgg
    11941 gtcggtgcgt agaggttgtg cagcagatca atctgacgcg ggttgatcag cgatttgccg
    12001 tcaaagccca gctgtttgat gtgggcggct tcttgcagga atccggcttc gttgttagcg
    12061 tcggaataga cggtatcgaa cgcctgaata cccgcagagc gcgcggcctg caaaatggaa
    12121 cagcgtgcga acagcagttc agttccttcc ggggagcgtt ctgtacgcag gttgcgcaca
    12181 tagtcttctg caccgagggc gataccgatc aaacgctcgg aagcgtgagc gatttccact
    12241 gcgcgggtaa tgcccagcgg agattcaatc gccgccagca ggccggtgct gccgggttca
    12301 cgaccacagg ctttttctat acgcaggatc tctttttcaa tatccagaac atcctgagcg
    12361 gtatcggttt tcggcagacg cacaacgtcc gcaccaccgc gaacgacggc ttccaggtcg
    12421 ttaacacccc attcggaatc cagcgcgttg acacgcacaa tggtttcaat atcgcgatac
    12481 agcggatgtt gcagcgcgtg ataaaccatg cggcgggcgg tgtctttttc acgtaatgcc
    12541 acggagtctt cgaggtcaaa catcagggca tcagccgggt agatgaagga gttgctgacc
    12601 atcgcggcat tggcaccagg cacaaacaac atgctgcggc gggtgcgagt tttacgttgt
    12661 tgcagcgaag cggaaatcat tggcaatcct cccatggcag agccgggata ccgctggcgc
    12721 gtgccagcag ggcttccagt cgtgcacgta aaatgcagtc cagtgcgcct ttgtcatcga
    12781 cattcagctg tacgccgcgc acgttgtagc gggcgagaac gtccagaatg gtggtgcgaa
    12841 ttgcatcgcc aaactgtttc tcaacgctgc tattgatttg caggtcgata tcctgcgtat
    12901 cgagtggggc gatgcgtatc atcacatccc cagactcaag ggtgcctgca acggcgggct
    12961 ggtttatttt catttttcac ctgtttctca tgcgggggtc ttttgacgag ctgccgcgtc
    13021 ctggcgggag tgctcaagca ggttctgcaa ataatgcagc gtgactgcag ggaccagcgg
    13081 cgcgatagcc gtgagatcgt ttttcgccag cagttgacgt acccgggaag cggatatcgg
    13141 catctcctgg taacgcagcc gctcaatttc aaccagttcg atgggcgctg cggagatagt
    13201 cggcgtttcc agccagtagc gcatatcctg gttgtactgg gcggtaacgc gacaaaaggg
    13261 ttcagtaccg acaaagcggt gagttacacc aagcgcggga gcgaggtact gacggaaaat
    13321 cttcagatca atttcggtgt aacaatggtt aatgacgctc tgttctttaa tgaagtagca
    13381 agggaacgta gcgcgggaga tgatgtattc tgagccgcga tgcacagtca ggcgtggaat
    13441 atcggcggtg ccttttaaca ccagatccag ccggtcttca taggggaagc gtgaagaatc
    13501 ttctttgact aaaaacagat gcaaccagtc gcactgtgcc gcagcttgtt gaatcagata
    13561 acggtgacca ttcgtaaagg gattggcgtt catcacaatg cagccaatct tgttccctgg
    13621 atgacgaaat tttttcagcg attcggcata gcgtttcagt cgcgtggcgc tgttttccat
    13681 cagcaccatc acgccgggta cgcaggtcag cgtggaaaaa ccgcactggc ggaacagcgc
    13741 ctcgtattcg gtt
//
LOCUS       DADLSA010000100        13642 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.100, whole genome shotgun sequence.
ACCESSION   DADLSA010000100 DADLSA010000000
VERSION     DADLSA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.100"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..741)
                     /locus_tag="JN691_002902"
     CDS             complement(<1..741)
                     /locus_tag="JN691_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY7244304.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPISFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIA"
     gene            complement(934..2124)
                     /gene="glpC"
                     /locus_tag="JN691_002903"
     CDS             complement(934..2124)
                     /gene="glpC"
                     /locus_tag="JN691_002903"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAY7244305.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(2121..3380)
                     /gene="glpB"
                     /locus_tag="JN691_002904"
     CDS             complement(2121..3380)
                     /gene="glpB"
                     /locus_tag="JN691_002904"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAY7244306.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRVTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFAPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(3370..4998)
                     /gene="glpA"
                     /locus_tag="JN691_002905"
     CDS             complement(3370..4998)
                     /gene="glpA"
                     /locus_tag="JN691_002905"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAY7244307.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGGRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            5271..6629
                     /gene="glpT"
                     /locus_tag="JN691_002906"
     CDS             5271..6629
                     /gene="glpT"
                     /locus_tag="JN691_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAY7244308.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            6634..7710
                     /gene="glpQ"
                     /locus_tag="JN691_002907"
     CDS             6634..7710
                     /gene="glpQ"
                     /locus_tag="JN691_002907"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY7244309.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7752..7994)
                     /locus_tag="JN691_002908"
                     /pseudo
     CDS             complement(7752..7994)
                     /locus_tag="JN691_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            8156..8812
                     /gene="inaA"
                     /locus_tag="JN691_002909"
     CDS             8156..8812
                     /gene="inaA"
                     /locus_tag="JN691_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HAY7244310.1"
                     /translation="MTGDSSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGK
                     KLYVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRA
                     LLVTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRH
                     IYVKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM
                     "
     gene            complement(8866..9120)
                     /gene="yfaE"
                     /locus_tag="JN691_002910"
     CDS             complement(8866..9120)
                     /gene="yfaE"
                     /locus_tag="JN691_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAY7244311.1"
                     /translation="MARVTLRITGTQLQCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(9120..10250)
                     /gene="nrdB"
                     /locus_tag="JN691_002911"
     CDS             complement(9120..10250)
                     /gene="nrdB"
                     /locus_tag="JN691_002911"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HAY7244312.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHQTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(10484..12769)
                     /gene="nrdA"
                     /locus_tag="JN691_002912"
     CDS             complement(10484..12769)
                     /gene="nrdA"
                     /locus_tag="JN691_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAY7244313.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         3236 a         3338 c         3644 g         3424 t
ORIGIN      
        1 agcaatagtc attaatctct ccttttgtag tggtgaacgt tcggcaatct cctgaataaa
       61 acgggtgaaa cgggaggtat cgccgcattg cagcagataa ttaaacagtg tttgtagctg
      121 gctgtcatta gtgaagcctc taaccaaaag cgtggtgatc ctgtcgacca tgccgattaa
      181 atcgtggtcg cgaatatgct tttgaatcag ttccagcaga gctatacgcc gatgttgcat
      241 gatctcatcg tcaggtacga tggtgatatc caccaacgga aaagcttcgg tgtacaactg
      301 ccgggcaagt tgcggatcgt caaactcatc cagccagtta agtgagtacg ggtagggcga
      361 tgtttcgcca tgataaaaca gcaatggcac caccagcgga actctgtcat agcctttatc
      421 taggtgacgc tgcatggcgg cagtggcata gcgcattggc cgaaaagcca tattcttttc
      481 tgcagagctt tgatgttcaa tcacgcagta gatatagccg tcaccgtcgc tggtttccac
      541 ggaccacaaa acatccgagt agtaagcgcg taaacttttt tcaataaaac taatgggttc
      601 caggcgtaag gtttgcaggt tgcaaagctt gcgcagtggt tctggtaaat gtatttcgag
      661 aaaatcccgt gcggtttcgg gtgtgaatat aaaggtttta aataccgcat catgcgggga
      721 ggaggttgtt gattcggtca tggttgtccg tcacctgata tttatgatga cgcgacatta
      781 cggttatccc aggcctttga catctgccag tttcagcctt tgcgagacgt tttcagagga
      841 attttcactg cgttgcaacg catacattgg gcacggaatc gtgtacgcaa aaatgcccgg
      901 taaccacatg cttaccgggc atcagaaggg agtttaagcc agcgcctggg ccagtagcgt
      961 aatcggatgt tcgcagcgaa gacttgtgga catctcaatc tgccatttac aggtttcgca
     1021 gtcggtgacc accagatctg cgccgctttc ttctatctgg cggaacagtg gtgcgccgat
     1081 ggcttgtgag gtggggtagt tctctttttt gaaaccgtaa gtacccgcaa taccgcagca
     1141 ctgggaatcc agcaccgtta actcaagccc cgggatgtta cgcaacagct ccagggtgta
     1201 gagcgtccag cccatttttt ccatatggca cggagtgtga taaaccactt tcagcggcag
     1261 cggtttcagc ggtaacgttt tgccttcgtc cagcttgcgc cacagccagc gggttgccag
     1321 ttcgatatga tcgcgcaagc ctttgttgtc gacattcagc acttccgggt attcgtcgcg
     1381 cagggcaaat gtacaggttg aggaggtggc aatcaccgga atgcctttta ctcccacagc
     1441 ttcgcggatc gactctacat tcgtaattgc ctgtttgcgt gctttatcgg taaagccgtt
     1501 ggcgattagc ggtacgccgc agcatttttc tttgctgagc agttgtacac cggtacccat
     1561 tgcgttgagc actttaatta aatctttacc taactgcgga tggttgtagt taacgaagca
     1621 gccgtgaaag aaagcgacct ggtctttata ttgtgcttgc tgagccgcta tgctgcgata
     1681 ccagcgacgg aacgtgccga aggagtattt cggtagcgtg cgacgatgat cgatttttaa
     1741 cgccgcatca agcagctgcc gcaccggttt cagcgaggta gcggtgttga cgattggtgc
     1801 gaacggcgtg gaaacgctac ccatcaggtc ggtatgactc aacacaaaat tacgcagcga
     1861 cgggcgcgtg gtgtcatatt tcgcccgcgc gcgctggata atatcgccaa tcttcacatc
     1921 ggacgggcag gcgacttcac aacgtttgca gttgatgcaa tatttcagcg cctcgtcata
     1981 cagtgcgcca tctttcaaac gcagacgctc gccatccggc ccggcttgtt ttggccctgg
     2041 ataaccggga ttcaccctgc tcaccgggca ggcggtggtg cagacggtgc acttaatgca
     2101 gttttcgaag ctggtgtcgt tcattgttgg cctcctgcgc gttgggcaat ctgttgagcg
     2161 gcatgtaaag cactgacggc acaaacaccg ccgccgcatc cctgggcgat gggagcaaat
     2221 ccgcccagca ccgaaccgat ggcaaacagg ttctcaatgg tttgccctgc ctgcgacggg
     2281 cgtagcgtct catcagtggt tacaccgaac tgctgccacg gttgcggcgc aaaaaaatcg
     2341 cccttatacc attcaccccg cgtggcggtt tgtagcacat caaggccgag aatcggctcg
     2401 cgaatgccgt tacgttccgc taccagtccg ccactaaaga aactgccgct ggcgagaacc
     2461 gcgaaacgtg gacgtagcgg aatatcggcg tgattgcggg tccagatttc attcactacg
     2521 ccatttttac aggtcacttt tttcacttca tcgcccggca tccacacgcc accctggcgc
     2581 acaaactggc gctgtaactg gttttgcaga cgaatgccca gcacggaagg cggcagcgtt
     2641 ggcaaaagca tcagtgaaca aggtagtttt tcattcaacc aacgccacag tttgtcatcg
     2701 gccaggccga agcaggcggg catcaggatc atttcgcagg tattggcgac aggaataagc
     2761 gcatcaagta acagcggcca gttttcttca ttatcaagga aacgggcgat attcaccgcg
     2821 cgaaattcgg tggcgttatt gcgcaggaca tccagttccg gcagctctat ttctgcggtt
     2881 tcaaccgaaa gatccagttc gcgcaacgaa gccgccgcaa gatgcgcctg gaaatccatc
     2941 agcccgctta tccccactac acatatcttc ttcgcgggta gcggccagac gggaacttct
     3001 ggcgaactta accaggttac acgcaaagtg ccgagcggcg taatccgctg gtgagccagt
     3061 tctacgctgc cctgcaattg cgcaccgctt tcagcgatta atgcctgcgc ctggcaagcg
     3121 agatcgagca cgcgttgtgg cccgagaaga gagtaaggat gtgcaggtgc ctgctgacgc
     3181 aaagattcca gtccactgtg aatgtctgcc acgggttgac catctggcag atggctcagc
     3241 aaatccagcg aaccggatga gaaatgcaac gcgctttgac cacgcgtgac aatggcacag
     3301 cgcaggccgt gtttttgcag ttgcaggcca cagagtaatc cggcgaggcc gccgcccata
     3361 atgacagtat caaagcgcat ctttctgctc cttctccaga ccacacagtc cctgataaac
     3421 ccagcgggta aattcgcttt cgcgcagtgc gtctccccag gcgatgggtt gcacgccttt
     3481 ccagcgttcg ttaaggaagg tggaaagttg ctcgatagat tgcgcggacg tcgtgacgtt
     3541 aaaccgttgc agcagtccgg cagcgcggca ggcgcagagt tcgccctggc aggtgcccat
     3601 ccccacacgg gtacgacggc gtaaatccag caggctatta acgtttaaat tttctaccgc
     3661 gtactgcact tcacccgcag ttaccgcttc gcactcacat accaggctac ggtgcagacg
     3721 gccttcgctc agccaggcag gcgtgcgatc gccatgacga taaaccgcag aaccgcgcag
     3781 cggggcaggc agggagatga ctttacgcaa ggtaacttca gcgggttctt gtgaaccagg
     3841 cagtgccaga tcggcagtcg tacaggggcg cgtgttgccc agtttgcggc ataccgcgtc
     3901 ggtagcccat tcagccatca gccgataggt catcagtttg ccaccggtga tggtgataaa
     3961 tccgtccaga ccatcgcgtt cagcatggtc gagcagcacg atgccacggc tgacgttacg
     4021 tccgctcggg tcgtcatcgc tggcaaccag cgggcgcacg ccagaatagg cccgcaaaat
     4081 gcgcgttttc gccatcacgg gggccagttt ttccccttca cgcagcagaa tatcaacctc
     4141 ttctgccgtc actcgattat cgtcaatctc gttgtaatca atacgtaaag aggtggtacc
     4201 aatcagcgaa atggtatcgc caggcaccag aatatcggcg tcggaaggtt tacggcagcg
     4261 gttgatcaca tgctggttaa tgcggtgatc catgatcagc agcgatcctt tcgccgggaa
     4321 catgcgaatg cgcagatcgg catattcggc aatgtgttgc ccccagatcc cagcggcatt
     4381 aaccacgaca ggtgcatgaa gggcctgagt ttcgccggtg agatggttac gtacacgacc
     4441 accgcacacc gtcgcgcctt cacgaatcag ccccgtgact tcatgagcgg taaggataac
     4501 ggcaccgtgt tctttggcat ccagcatgtt tgctgcggtc agacgaaatg gatcaacggt
     4561 gccatccgga actttcaccg cgccaatcag tgccgggtta acggcaggtt cgataatgcg
     4621 cgcttgctgc gggtctatag cttctgcgct gatccctgct tcttcgcagg cgcgaataaa
     4681 agtggcctgg aatgagaggt catcttccgg cagggtgata aacaggccgt tggttggttc
     4741 aacgcagtga cgtgcaatgc gtttcaggat ctggttttca ctaatgcatt cgcgggccga
     4801 ttccgcatcg gttactgcat agcgcgcacc gctgtgcagc aggccgtggt tacgcccggt
     4861 ggcaccggtt gcgatgtcgt ggcgctcaac caaaatcacg cgcagcccgc gcagggcaca
     4921 gtcgcgggca atcccggctc ccgttgcgcc gccgccaatg ataatcacgt cacttgattg
     4981 cgagtcgcga gttttcattg ttatccctct gaagttcgtt ttttaccatt tagccatagt
     5041 aaaaacataa attgtttgat ttcgcgcata ttcgctcata attcgaaagt gaaacgtgat
     5101 ttcatgcgtc attttgaaca ttttgtaaat cttatttaat aatgtgtgcg gcaattcaca
     5161 tttaatttat gaatgttttc ttaacatcgc ggcaactcaa gaaacggcag gttctctcac
     5221 tgaatcaggc tgttaatcat aaataagacc acgggccacg gaggctatca atgttgagta
     5281 tttttaaacc agcgccacac aaagcgcgct tacctgccgc ggagatcgat ccgacttatc
     5341 gtcgattgcg ctggcaaatt ttcctgggga tattctttgg ctatgcggct tactatttgg
     5401 ttcgtaagaa ctttgcgctt gctatgcctt atctggttga gcagggattc tcacgcggtg
     5461 atttaggttt tgccctttcg gggatctcga ttgcttatgg attttcgaaa ttcatcatgg
     5521 gttcggtatc ggatcgctcg aatccgcgcg ttttcctgcc cgcaggtttg attctggcgg
     5581 cggcagtgat gttgtttatg ggctttgtgc catgggcgac gtcgagcatt gcggtgatgt
     5641 ttgtactgtt gttcctctgc ggttggttcc aggggatggg gtggccgccg tgtggtcgta
     5701 ctatggtgca ctggtggtcg cagaaagaac gtggcggcat tgtgtcagtg tggaactgtg
     5761 cgcacaacgt cggtggtggt attccgccgc tgctgttcct gctggggatg gcctggttca
     5821 atgactggca tgcggcgctc tatatgcctg ctttctgcgc cattctggtg gcattattcg
     5881 cctttgcgat gatgcgcgat accccgcaat cctgtggctt gccgccgatc gaagagtaca
     5941 aaaatgatta tccggacgac tataacgaaa aagcggaaca ggagctgacc gcgaagcaaa
     6001 tcttcatgca gtacgtgctg ccgaacaaac tgctgtggta catcgccatc gccaacgtgt
     6061 tcgtttatct gctgcgttac ggcatcctcg actggtcacc gacttatctg aaagaggtta
     6121 agcatttcgc gctggataaa tcctcctggg cctacttctt ctatgagtat gcgggtattc
     6181 cgggcaccct gctgtgcggc tggatgtcgg acaaagtctt ccgtggtaac cgtggggcaa
     6241 ccggcgtctt ctttatgaca ctggtgacca tcgcgactat cgtttactgg atgaacccgg
     6301 caggtaaccc aaccgtcgat atgatttgta tgattgttat cggcttcctg atctacggtc
     6361 ctgtgatgct gatcggtctg catgcgctgg aactggcacc gaaaaaagcg gcaggtacgg
     6421 cagcgggctt taccgggctg tttggttacc tgggcggttc ggtggcggcg agcgcgattg
     6481 ttggctacac cgtggacttc ttcggctggg atggcggctt tatggtaatg attggcggca
     6541 gcattctggc ggttatcttg ttgattgttg tgatgattgg cgaaaaacgt cgccatgaac
     6601 aattactgca aaaacgcaac ggaggctaat ggcatgaaat tgaagctgaa aaaccttagc
     6661 atggcgatca tgatgagcac tatagtcatg ggaagcagtg caatggcggc ggacagcaac
     6721 gaaaaaatag tcatcgccca tcgcggtgcc agtggatatt tgccggagca tacgctgcca
     6781 gcaaaagcga tggcgtatgc gcagggagcg gattatctgg aacaggattt ggtgatgacc
     6841 aaagacgacc atctggttgt tctgcatgac cattatctcg atcgtgttac tgatgttgcc
     6901 gatcgtttcc cggatcgggc gcgcaaagac ggtcgttact acgcgataga tttcacgctg
     6961 gatgaaatta agtcgctgaa atttaccgaa ggtttcgata ttgaaaacgg taaaaaagta
     7021 cagacttatc cggggcgttt cccaatgggt aagtccgact tccgggtgca cacctttgaa
     7081 gaagagattg aatttgttca ggggttaaat cactctaccg ggaaaaatat cggtatctat
     7141 ccagaaatca aagcgccgtg gttccatcat caggaaggga aggatattgc ggcaaaaacg
     7201 ctggaagtgc tgaagaaata tggttacacc ggtaaagacg ataaagttta tttgcaatgt
     7261 tttgatgctg atgagctgaa gcgtattaag aatgagctgg aacccaaaat gggcatggag
     7321 ctcaatctgg tacagctgat tgcctatacc gactggaatg aaacgcagca gaaacagccg
     7381 gatggaagct gggttaatta caactacgac tggatgttta agccgggtgc catgaaacag
     7441 gtggcggaat atgcagatgg tattggtccg gattaccata tgttgattga ggagacatcg
     7501 cagccgggta atatcaaact cactggcatg gtgcaagatg ctcagcagaa taaactggta
     7561 gtgcatcctt ataccgtgcg gtcagataaa ctgcctgaat acactcctga tgtgaatcag
     7621 ttatatgatg ctctgtataa caaagcgggt gtaaatgggc tgtttactga tttccctgat
     7681 aaggcagtaa aatttcttaa taaagagtaa tgttactgca ggatggcgtt catgcgccat
     7741 cctgtctcat atcaaatttt tccaatcgcc actaattgtt ccatgcacat ttgtagtaaa
     7801 aacagtggac tgccttctac cggcttttct ttagtcagca acgaaatctg tcgatagaag
     7861 gttggttcgt gaggaacgga acacaattca cctgcaatgc ttttcagcgt taactctggt
     7921 tgtaaggcga tacctaatcc ctgtcgaata aaattcatcg ctgtatccga ctgattaaat
     7981 tcacatttta tctgctgggc gcactattag tggtagtggc tggtggaatc tatgcgctgc
     8041 aaatgaaggg caaaagcggt gtcacacctg tagcggcaaa aaaaattcat taatacgaca
     8101 cgtttcatta agattttcct caggtaacca cctatagtca ttgtcgttaa taaaaatgac
     8161 aggcgatagt tcagcaaagt acgacgaatt taatcactgg tgggcaacag agggcgactg
     8221 ggttgaagag cctaactatc gccgtaatgg tatgagcggg gtgcaatgcg tcgagcgcaa
     8281 cggcaaaaag ctgtatgtaa agcgcatgac gcatcatctg tttcattcag tacgttatcc
     8341 gttcggccga ccaacgattg tccgtgaggt tgcggtaatt aaagaactgg aacgggccgg
     8401 tgtcatcgtg ccgaagatcg tttttggtga agcggtgaaa attgagggtg aatggcgcgc
     8461 gttgctggtg actgaagata tggcggggtt catcagcatt gctgactggt atgcccggca
     8521 tgcagtatcg ccttattctg acgaagtacg gcaagccatg ttgaaagcag tggcactggc
     8581 gtttaagaaa atgcatagcg ttaatcgtca gcatggctgt tgctatgttc gccatatcta
     8641 tgtgaaaaca gaaggtaagg cagaagctgg ttttcttgat ctggagaaaa gtcgtcgccg
     8701 cttgcgtcgc gataaagcga ttaatcatga cttccgccag ctggaaaaat atctggagcc
     8761 gatccctaaa gcggactggg agcaggtgaa agcgtattac tacgccatgt aatcgttgta
     8821 ggccagataa tacgcgtaag cgtcgtatct ggcgctagta cccgatcaca actcgatttc
     8881 aatatcgcct tttgcccggc aacaacaggg caaaatttcc cccggctgaa taaaggctaa
     8941 cggttcggca atccagtcca cctgacctgc gaccagtcgg gtgcgacagg agccgcagta
     9001 accttcgcgg cactggtact caaccgccac attgtgggat tccagcgccg ccagaaggga
     9061 agggtgttca tcctggcact gcagttgtgt gccagtgatg cgcagggtaa cgcgggccat
     9121 cagagctgga agttactcaa atcgtcggtg tccacttccg cgtcaatctg cccgaccaga
     9181 taagaactga cttccacttc ctgcggagca acctgcacgt tatcagacac cagccaggtg
     9241 ttgatccacg ggatcgggtt ggagcgcgtc tggaacggca gatccaaacc gactgcctgc
     9301 atgcggatat tggtgatgta ttcaacgtac tggcagagaa tatctttatt cagaccaatc
     9361 atcgaaccgt cacggaacag ataatccgcc cagtctttct cttgctgtgc cgcctggaca
     9421 aacaggtcat agcactcctg cttacattct tcggcaattt ccgccatctc cggatcatcc
     9481 gcgccgctgc gcagcagatt cagcatatgc tgagtaccgg tctggtgcag ggcttcatcg
     9541 cgggcaatca ggcgaataat cttggcgtta ccttccatca gttcgcgttc tgcaaaggca
     9601 aaggaacaag caaagctgac gtagaagcgg atcgcttcca gcgcgttaac gctcatcagg
     9661 cagagataca gttttttctt cagctcgcgc aggctaacgg ttacggtttt accgttaacg
     9721 gtgtgggtgc cttcgcccag aaggtgccag tagctggtca tttcgatcag ttcatcgtaa
     9781 tagctggaga tcccttccgc acgtttctgg atctgctcgt tggtgacgat atcgtcaaac
     9841 acaacagacg gatcgttaac gatattacga atgatatgag tataggaacg ggaatgaatc
     9901 gtttctgaga acgcccaggt ttcgacccag gtttccagtt ccggaataga aataagcggc
     9961 aatagcgcca cgttcgggct acgaccctga atggaatcca gcagcgtctg atatttcagg
    10021 ttgctgataa agatgtgttt ttcgtgctcc ggcagcgcct ggtaatctat acggtcgcgg
    10081 gagacgtcaa cttcttccgg acgccagaag aaagagagct gcttttcgat cagcttttcg
    10141 aagatgtcat atttttgctg atcgtagcga gccacgttga ccggctgacc aaagaacatc
    10201 ggttctttga gctgatcatt tttcgtctgt gaaaaggtgg tatatgccat gagtgtgtcc
    10261 tgttgggagt ttaatgccgg ataaggcatt ttacgccgca tcctgtgcct gatgcgacgc
    10321 tggcgcgtct tatcaggcct acaaatccga gccgtaggcc ggataaggcg ttcacgctgc
    10381 atccggcacc cggagcctga tgcgacgctg gcgcgtctta tcaggcctac aaaccgagcc
    10441 gtaggccgga taaggcgttt acgccgcatc cggcatctca atatcagatc ttacatgcgc
    10501 cgctttcgca gccatcgtcc tggattgacg gtaccagatc gtcctgcgcg tcttcagcgc
    10561 cgtcacgggt gttctgataa tacagcgttt tgaccccgaa tttgtaggcg gtgagcaggt
    10621 ctttcagcaa ctgctgcatc ggcacttttc ctgacgggaa acgtgacgga tcgtagttgg
    10681 tgttagcaga gatcgactga tcgataaatt tctgcatgat acctaccagt tgcaggtagc
    10741 catcgttacc cggcatttcc cacagcagct cataggcgtc gtgcaggtgc tcgtagtccg
    10801 gcaccacctg gcgcaaaata ccgtctttcg acgctttgat gctgacgtaa ccgcgcggcg
    10861 gttcaatacc gttagtggcg ttagagatct gcgaagaggt ctcggacggc atcagggcag
    10921 aaagcgtgga gttacgcagg ccgtgcgttt tgattgattc acgcaatgct tcccagtcgt
    10981 aatgcagcgg ctcattagcg atggcatcca gatctttctt ataggtatcg atcggcagga
    11041 tccctttcgc gtaagtggtt tcgttaaacc acgggcacgc gccttgctct ttagccagct
    11101 cattagacgc tttcagcagg taatactgaa tcgcttcgaa ggttttatgc gtcaggttgt
    11161 tggcgctgcc gtcggagtag cgtttaccgt gcttcgccag atagtaagcg aagttaatca
    11221 caccaatacc cagcgtacga cgccccatcg ctccacgttt ggcggccggg atcgggtaat
    11281 cctgataatc cagtagcgcg tcaagtgcac gaaccgccag aatcgccagc tcttccagtt
    11341 catccaggct attaattgcg cccaggttga aagcagacag cgtacacagc gcgatttcac
    11401 cgttctcgtc gttgacgtcg ttcagcggtt tggtcggcag ggcaatttcc aggcacaggt
    11461 tagactggcg aactggcgcg atggtcggat caaacggact atgggtatta cagtggtcaa
    11521 cgttctgaat atagatacga ccggtagacg cacgttcctg catcatcagc gagaacagct
    11581 caacggcttt cacacgctgc ttgcggatgc tgtcgtcttt ctcatatttg gtatacaggc
    11641 gttcaaactc ttcctgatcg gcgaaaaacg cgtcgtacag ccccggtacg tcggacgggc
    11701 tgaacagggt gatatcttca cctttcagca ggcgggtata catcagtttg ttgatttgta
    11761 ccccgtagtc catatgacgc acgcggttgc cttcaacccc acggttgttt ttcaacacca
    11821 gcaggctttc cacttccaga tgccacatcg ggtagaacag cgttgccgca ccgccgcgca
    11881 caccgccctg agagcaggat ttcaccgctg tctggaaatg tttgtagaac gggatacagc
    11941 cggtatggaa cgcttcgcca ccgcgaatcg ggctgcccag cgcacgaata cgtccggcgt
    12001 tgatgccgat cccggcacgc tgggaaacat atttcacaat cgcgctggag gtggcgttga
    12061 tggaatccag gctgtcaccg cactcgatca gtacgcagga gctgaactga cgagtcgggg
    12121 tacgcacgcc ggacatgatt ggcgtcggca gcgaaatttt aaatgtggaa accgcgtcgt
    12181 aaaaacgctt cacgtattgc aggcgcgttt cacgcgggta gttcgagaac aggcacgcgg
    12241 caacaagaat ataaaggaac tgggcgcttt cgtagatttc gccggtcacg cggttctgta
    12301 ccagatattt gccttccagc tgcttaacgg cagcataaga gaaggtcata tcacggtcgt
    12361 gatcgataaa cgtgtccatc tgcttgaact cttcttccgt gtagtcttcc agcagatgat
    12421 tatcgtattt gcccatctcg accattttca ccacgtggtc gtacagcgca ggcggctcaa
    12481 actggccgta ggctttttta cgcaggtgga agatcgccag gcgcgcggcg agatactgat
    12541 aatccggcgc atcacgggag atcaggtctg cggcagcctt gataatagtt tcgtggatgt
    12601 cagaggtctt gataccgtca taaaactgaa tgtgggagcg cagctcgacc tgggaaatcg
    12661 aaacgttatg cagtccttct gccgcccaat ccagaacgcg atggattttg tcgagattga
    12721 tgcgctctgt gctaccgtcg cgctttgtca ccagcagatt ctgattcatg tatgtcgtac
    12781 ctgtttttgg aattctttcc gcaatagttt catgcagact acctgtagta agggaagctg
    12841 cttagaacac tatatatagg gggtatgttt gggattcact gcaagatagt gtgaaaatga
    12901 ccctcttgca agtgcataac tttgtggata actcaggaag gaaaaagtgg ctttcgcgca
    12961 ccttaggtca gacaaggtgt ccgggaaagt caatgggaag agaaaaattt gttaaaaata
    13021 actgttcggc gatttcttga acggcagaat aggttggata aattgatata gatggtttat
    13081 taatcttttt gctaatgatt gcaaaaattg atctaagcac gagtcatcac ataatctgat
    13141 gtatcagttg tggtcaatat ataagcgaat gtaagcgatt gttgatgtcg aaatggttgt
    13201 tttctattcg gttattgtta aatgcgaagc cattttgtta atttacctta tattataaga
    13261 aaattattca ttactttaat ttagtttaaa tataactggt atcccttaag gttattaact
    13321 tgaattatga cgcactgata ttattcatca aataataaca aaatagccat tgcatcgggt
    13381 tggacaattt acaaaagaaa gataacccat attgtataat gcgtttaaat atgccgtctt
    13441 atataaaatg atggtcagat taatatgcgg attatctttc tacgcaagga gtatttatct
    13501 ctactcccgt caatgattgc atctcttttc tctgctaacg gtgtcgcggc ggccattgat
    13561 ttatgccagg gatatgatat caaagcgagt tgtcacgcca gcaggcaaag cctttcaggc
    13621 attacgcagg actggagtat cg
//
LOCUS       DADLSA010000101        13585 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.101, whole genome shotgun sequence.
ACCESSION   DADLSA010000101 DADLSA010000000
VERSION     DADLSA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.101"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..896)
                     /locus_tag="JN691_002913"
     CDS             complement(<1..896)
                     /locus_tag="JN691_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7244314.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGG"
     gene            1137..1937
                     /locus_tag="JN691_002914"
     CDS             1137..1937
                     /locus_tag="JN691_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY7244315.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2015..2785
                     /locus_tag="JN691_002915"
     CDS             2015..2785
                     /locus_tag="JN691_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7244316.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYFAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2833..4191)
                     /gene="mltD"
                     /locus_tag="JN691_002916"
     CDS             complement(2833..4191)
                     /gene="mltD"
                     /locus_tag="JN691_002916"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAY7244317.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4263..5018)
                     /gene="gloB"
                     /locus_tag="JN691_002917"
     CDS             complement(4263..5018)
                     /gene="gloB"
                     /locus_tag="JN691_002917"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAY7244318.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5052..5774
                     /locus_tag="JN691_002918"
     CDS             5052..5774
                     /locus_tag="JN691_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7244319.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5771..6238)
                     /gene="rnhA"
                     /locus_tag="JN691_002919"
     CDS             complement(5771..6238)
                     /gene="rnhA"
                     /locus_tag="JN691_002919"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAY7244320.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWLHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6303..7034
                     /gene="dnaQ"
                     /locus_tag="JN691_002920"
     CDS             6303..7034
                     /gene="dnaQ"
                     /locus_tag="JN691_002920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAY7244321.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7167..7243
                     /locus_tag="JN691_002921"
     tRNA            7167..7243
                     /locus_tag="JN691_002921"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7201..7203,aa:Asp,seq:gtc)
     gene            complement(7374..8420)
                     /locus_tag="JN691_002922"
     CDS             complement(7374..8420)
                     /locus_tag="JN691_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HAY7244322.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFTRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8431..9346)
                     /gene="tssG"
                     /locus_tag="JN691_002923"
                     /pseudo
     CDS             complement(8431..9346)
                     /gene="tssG"
                     /locus_tag="JN691_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410578.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9463..11346)
                     /gene="tssF"
                     /locus_tag="JN691_002924"
     CDS             complement(9463..11346)
                     /gene="tssF"
                     /locus_tag="JN691_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HAY7244323.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11362..11856)
                     /gene="tssE"
                     /locus_tag="JN691_002925"
     CDS             complement(11362..11856)
                     /gene="tssE"
                     /locus_tag="JN691_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HAY7244324.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11853..12683)
                     /locus_tag="JN691_002926"
     CDS             complement(11853..12683)
                     /locus_tag="JN691_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HAY7244325.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTALACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12670..13584)
                     /gene="tagK"
                     /locus_tag="JN691_002927"
                     /pseudo
     CDS             complement(12670..13584)
                     /gene="tagK"
                     /locus_tag="JN691_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3106 a         3545 c         3627 g         3307 t
ORIGIN      
        1 cctccgggag ctgtttttac atgctcgcta aggaaatcga taaaagcccg gatgcgcgta
       61 cttaccgcac ggtcgctgta atagacggca ctgaatggca tttccactgg caacacttta
      121 tctgccatta actccaccaa ttctccgcga gcgatttctt tgtcgatcat gtagtcggac
      181 aaacacgcaa tcccgttgcc actcaggcaa agctgtttca gtgtttcccc actattggat
      241 gacaaaccgt acttcacctc atgtaattgt ccatcactac aggctatcgg ccaggtattg
      301 agggaagcgg gttcagtgaa tcccaggcaa acatgttgct ttaaatcgtc gatcgtttct
      361 ggcttcccgt agcgggaaat ataatcgggg gaggcgataa tttttcgata actgttaaat
      421 aacggcctgg cacgtaagct ggagtccgtt aacgtaccag cgcgtatcgc gacatccact
      481 tttctttcga tcaaattaat aatcgtttcg gaggagacta gcgataaagt gacttccgga
      541 tagcgttcac ggaaaggctt aattaacggc atcagaaagt gcagcaccac tggagttgcg
      601 gcatcgatcc gtaacagtcc acgcggtgta ttacgcgtct ccataatttc tgattctgcc
      661 gctgccatct cctgcaaaat tgactgtacg cgacgaaaat aacgctcgcc ttcttccgtc
      721 aggctaagtt gccgcgtggt ccgattaagc aggctaacgc caagtttcat ttccagcttt
      781 ttcaccgccc ggcttaccgc tgagtttgct tgccctaatt gttccgctgc ccggctaaag
      841 ctgccgcttt cgacgaccga aacaaaaatg gcgagttctt ccgacgtggc tttcattttt
      901 gctcctgttg caaaaaagat gagatatttt gaatttattt gtcattaaac catcaagatg
      961 cgtgatatgt catccgattt aatgttctca ataatgagca aatttctgac cggagtaagc
     1021 acttgcttac ataacaatat acaattgctc gttgaaagag tgagctaaaa tccctataac
     1081 agtagaaccc tcccgagtgc ggaagggttg acgtaataga ggtttcaaag tcaaaagtgc
     1141 gaaaaaacac ctatgccatg cgctatgttg ccggacaacc tgcggaaagg atcttaccgc
     1201 cggggtcttt tgcgagcatc ggccaagcat taccacctgg agaaccgtta agtaccgaag
     1261 agcgtattcg gatcctggtg tggaacatat acaaacagca acgcgctgaa tggttgtcgg
     1321 tattaaagaa ctacggcaaa gatgcacatc tggtgttatt gcaggaagcg cagacaacgc
     1381 cagagttagt acagtttgcg accgctaact atcttgccgc cgatcaggtg cccgctttcg
     1441 tgctgccaca acatccttcc ggtgtaatga ccctttcggc ggcgcatcca gtgtattgct
     1501 gcccgttacg tgaacgagaa cccattttgc gtctggcgaa gtcggcactg gtgacggtct
     1561 atccattacc tgacacccgc ctgttgatgg tggttaatat acacgccgtc aacttcagtc
     1621 tgggcgtgga tgtctatagt aagcagttac ttcctattgg tgatcagata gctcaccaca
     1681 gcggcccggt cattatggcg ggagatttca atgcctggag ccgtagaagg atgaacgcgt
     1741 tatatcgctt tgcgcgggaa atgtcgctgc gccaggtgcg ttttaccgat gatcagcgcc
     1801 gtcgggcgtt tggccgcccg ctcgattttg ttttctaccg tggtctgaac gtcagcgaag
     1861 cttctgtatt ggttacgcgc gcttccgatc acaatccgct actcgttgaa ttcagtcccg
     1921 gcaagcctga taaataaggt atgtcaggtc tgccacaggg cagaccaacg tttggcgctg
     1981 tccttttata ctcaaacaac aaaaggacag cgcaatgaca acacaatccc accatgacca
     2041 cgtagaaaag cagtttagct ctcaagcctg cgaatatctg accagtaccg tacatgcatc
     2101 cggacgagat ttacagcggc tggcggtgcg tctggctgac tatcctggtg caagtgtact
     2161 tgatatgggc tgcggagcag ggcatgccag ctttgttgct gcgcaaaacg tgagcgcggt
     2221 ggtggcgtat gacttatctg cccaaatgct ggatgtcgtg gcacaagctg ccgaagcccg
     2281 gcaactgaaa aatatcacca cccgccaggg atatgccgaa agtctgccat ttgccgataa
     2341 cgcatttgat attgttatca gccgttattt tgcccatcac tggcatgatg ttggtgcagc
     2401 actgcgtgaa gtgaaccgga tattgaaacc tggcggtagg atgattgtga tggacgtaat
     2461 gtctccgggt catccagtgc gcgacatctg gttacagacg gtagaagcat tacgcgatac
     2521 ctctcacgta cgaaactacg ccagcggtga gtggttgagg ttaatcaatg aagccaatct
     2581 gatagttgat aatttaatta cagataagtt accgctggaa ttttgttcat gggtcgcgag
     2641 aatgcgtacg ccagaagcgt tagtagacgc tattcgcatt taccaacaga gcgcatcgac
     2701 agaggtgaga acgtattttg ccttgcagaa tgacggctct ttcaccagtg atatcatcat
     2761 ggtagaagca cataaagcgg cataaataaa aaaggcaccg ggggaatcgg taccttttta
     2821 ttatctggtt tgtcaggaat ctggcatgtt gttgtttttc acaaacaacg tcagcttgtc
     2881 gcctggttgc agattcgcag tgtcgctgtt ccagcgcatc acatctttga tgttcacgcc
     2941 gtggcgttta gcaatgcttg aaagcgaatc gcctttgcgc acacgatacg taatgctatc
     3001 gctgttgttt gccagtcgct gtgcgctact gcctgcacca atcgtcaaac tttggcctgg
     3061 cttcagctta gatccgcgca gtttgttcca ctgctgcaaa tctttggtgc ttacgccgag
     3121 acgtgaagcg atgcttgaaa gcgtgtcgcc agagcgtacg gtgtaaacac ggctgttaag
     3181 cggcgtattg tcggcaacca gcgtcgactg tacagcagca atttcgcctg aagcgagaga
     3241 ttcacgcagt tgatctgcat gcttctttgg caccatcacg tactgcggcc cacttgcgcc
     3301 cagcgtggag cctttcacgc cagcgttgaa tgtcttcagc ttgctgacgg aaatccccgc
     3361 catatctgca accttcgcca tttcaaccgg gctactcagg tgcacacgcg ccagagcacg
     3421 gctttcatcg gtcgttggca gacgtacgcc ataacgcttg ctgtttttga gaatatcact
     3481 caatgccagc attttaggca cgtactgctt cgtttcctgc ggcaacggta acgaccagaa
     3541 gtccgtggat ttcccacgcg ctttgttcgt tttaattgcc ttcatgaccc gaccttcgcc
     3601 gctgttataa gccgctacgg tcagaagcca gtcgccgtca aacattttgt tcagacgctg
     3661 catcatattc agcgcggcag ttgttgaagc aacaacatcg cgacgcgcgt cataattgcg
     3721 ggtctgtttc aaaccataat tgcgccccgt gctcggaatg atctgccaga tgcctgcggc
     3781 attggcgcca gacgttgcgt gaggatcaaa agcgctctcc actatgggta gtagtaccag
     3841 ttccataggc atgttacgtt ttttaacttg ccctgctatc cagtacatat acggctctgc
     3901 ccgtaaagtt acatcgtgga gatagctctt attgcgtaaa tatttctgtt tctgttcgcg
     3961 aatccggtca ttttccggaa ttcccatctt tagctcgtcg ccaatgaaag cccacaagtc
     4021 accatctggc gcgatagacg tcccatcgtc catccatcgt gcctgacttg taaactttgc
     4081 tgcttcccct tgaccagctg cagaaaggct ctgtgcgtgc tgttgaacgt tgccggtact
     4141 ctggcaaccc acgagcagga cagaggcgag taatatcgct tttgccttca tgtgtgtgtc
     4201 aatagttgct taaaagacga ccgatcataa cggcgaacgg agccgatgac aagaaagttt
     4261 tatcagaacc tatctttctt tgaccttaac catgcaaaac gctcttcagg ttgttgcaat
     4321 aatgtttctt cattaattac attaattaaa tcaatatctt ccgttcttaa aaaaacatta
     4381 atttgccgct catttttcag aattacgggt agtgttattt gattttttgc ccgtaactcc
     4441 ttaactttac gataataatc atttatggac aaatcgtgcg gaaaaatact caaagcaaac
     4501 ttcatatttg ataacgtata ttcatgagca caacatacca atgtatcgtc aggtaacgca
     4561 cttagctttt taagtgattg atacatttgt aaagctgtcc cttcaaacaa ccgaccacac
     4621 ccaccagaaa acagcgtgtc gccgcaaaat agataaggtt tactgaagta acagatatgt
     4681 cctaaagtgt gacccggtgt ggtaattaca ctaaattcat gccctaaaac gaaggcagtt
     4741 tcgccatctt tgactacctg tgttgttccc ttatcttgtg tctcttgtgg accatacacc
     4801 acaatttgcg gaaacttttc caccagttct tttacgccgc caacgtgatc gtggtgatgg
     4861 tgggttagaa atatggcctc cggttgccag ttattggcgg taatggcgtt taatactggc
     4921 tctgcgtctc cgggatcgac aatcaggcag cgacctgcct catcattcaa aacccagatg
     4981 taattgtcat caaaggcggg aatactgtta agattcatag attacctctc agtgtaaaac
     5041 ggaaggttgt gatgaaaccg gcaagagtcc ctcaaactgt cgtggctcct gattgctggg
     5101 gcgatttgcc ctggggagag ctttatcgca aggcgctgga gcgccagctc aacccgtggt
     5161 tcactaaaat gtatggtttt catctgctta agattggcaa tttaagcgca gaaatcaatt
     5221 gcgaagcgtg cgcggtttct catcaagtga atgtttctgc gcaaggaatg cccgtccagg
     5281 tgcaggcgga tccacttcat cttccttttg ccgataaatc cgttgatgtt tgtctactgg
     5341 cacatacctt gccgtggtgc accgatccgc atcgtttatt acgtgaagcc gatcgggtac
     5401 tgattgatga tggctggctg gtgattagtg gcttcaaccc catcagttta atgggattac
     5461 gcaaacttgt gccggtattg cgcaaaacct cgccctataa tagccggatg tttactctga
     5521 tgcggcaact ggactggctc tctttgttga attttgaagt gctgcacgcc agccgtttcc
     5581 acgttctccc gtggaacaaa cacggaggaa aactattgaa tgcgcatatt cctgcgcttg
     5641 gttgcttaca acttattgtt gcccggaaac ggactattcc tttaacgcta aatccgatga
     5701 aacagagtaa aaacaagcca cgaattcgcc aggcggttgg agccacccgg caatgtcgta
     5761 aaccacaggc ttaaacttcg acttggtagc ccgtatcttc cagtgtggga ttcatcgccg
     5821 cggcacgagc cagttcatca cagcgttcgt tttccgggtg tccggcatgg cctttaaccc
     5881 attcccattt gatttgatgc tgccccaatg cagcatcaag acgttgccag agatcgacat
     5941 tttttactgg ttttttgtct gctgttttcc agccacgttt tttccagtta tggagccact
     6001 gggtgatacc ctggcggaca tactggctgt cggtactcaa aatgacttcg caatgttctt
     6061 ttaacgcctc cagcgcgaca atagcggcca tcaactccat acggttgttg gtggtgcggg
     6121 tgtagcctgc gctaaaggtt ttctcgcgtc cgcgatagcg taaaatagcg ccgtaacccc
     6181 caggtccagg attacccaga cacgaaccat cggtgaaaat ttctacctgt ttaagcatct
     6241 ctggtagact tcctgtaatt gaatcgaact gtaaaacgac aagtctgaca taaatgaccg
     6301 ctatgagcac tgcaattaca cgccagatcg ttctcgatac cgaaaccacc ggtatgaacc
     6361 agattggtgc acactatgaa ggccacaaga tcattgagat tggtgctgtt gaagtagtga
     6421 accgtcgcct gacgggcaat aacttccatg tttatctcaa acccgatcgg ctggtggatc
     6481 cggaagcctt tggcgtacat ggtattgctg atgaattttt gctcgataag cccacgtttg
     6541 ccgaagtagc cgatgagttt atggactata tccgcggcgc ggagttggtg atccataacg
     6601 cagcgttcga tataggcttt atggactacg agttttcgtt gcttaagcgc gatattccga
     6661 agaccaatac cttctgtaag gtcaccgata gccttgcggt ggcgaggaaa atgtttcccg
     6721 gtaaacgcaa cagcctcgat gcgctatgtg cccgctacga aatagataac agtaaacgaa
     6781 cgctgcacgg ggcattactc gatgcccaga tccttgcgga agtttatctg gcgatgaccg
     6841 gtggtcaaac gtcgatggct tttgcgatgg aaggagagac acagcagcaa caaggtgaag
     6901 caacaattca gcgcattgta cgtcaggcaa gtaagttacg cgttgttttt gcgacagatg
     6961 aagagctggc agcacatgaa gcccgtctcg atctggtgca gaagaaaggc ggaagctgcc
     7021 tctggcgggc ataaatacat gtaaaaggcg ctaaaaatag cgacttgggc gatttttgca
     7081 gcaaacgatt caaaagatga gaaaaatcgt tgacgaaggt cgaggcaatc cgtaatattc
     7141 gcctcgttcc caacggaaca caacgcggag cggtagttca gtcggttaga atacctgcct
     7201 gtcacgcagg gggtcgcggg ttcgagtccc gtccgttccg ccaatattca gaaagcccga
     7261 atcagtaatg attcgggctt tcgtcgtttt ggtgaagtga gaatttttca ctccgaagta
     7321 aaacggatat gatttaaaaa atggccagac attataaatt ctggccactt ctgttactcc
     7381 agcgcatccc atttctccag cagttctact ggaatttcat ttattacctc cgagacgcgt
     7441 tttccgacca ggcgttctgc ctggcgcagc aaaggaatgg tcgggctgct cggttcgctg
     7501 tgctcaaacc agcgacggat gacacgcagg cgttccagcg cgtcgtttcg gtcgcggatc
     7561 tgccggggtt ctgcgccggt catcggtgat atttcctccg cgatatgcac cggtagcgca
     7621 atctgctctg cctgcatctg ttcagtatga acgatggtgg cgttttccgg ctgctcagcc
     7681 tgtggttgtg gcaggatctc cggcgtggtg ctctgcactg ctcccggtaa cagggccagc
     7741 agttgcgtca gacgggtgaa gtccggtgcc tggtcattca gcgtttcacg cgcatggcgt
     7801 tgcagccgct cagtggtctc tgctgcctgg cgaaaggcat ccagcggcag tgtgccgcgc
     7861 gcttccaggt ctgccagttg ctggcgtacc gattctggtg ccagcgcatc cgccgggcgt
     7921 aatgcagata gcgaacgctc cacatcccgc acctgaaggc gcatggcagc attatttgac
     7981 agtgtaatgc cacgaatatc cgccattaca ccttcgtggt ccagcaacgc cgccagcgcg
     8041 ttactgcgtg ccaccgcagc atcttcggcg gtgatatctt cagtcgcgag cagttgagga
     8101 tggatggcgt ccgggtacgt ggagcacaac gtttccagca gcgtgagcgc ctccgacagt
     8161 cccctggcac cggcctgctg gatacggcag cgcagcagga caaccagaat gcggatatct
     8221 ttgctgcgca tcagcagacg atgggcgtcc cgctcaagct ccgcccagtt aatgttttcc
     8281 ggcgtactga caaaatcgcc atattgcgct tcttcacgcg gtgcggcgcg ggtgaacagc
     8341 agcaggtatt ccggatcgta ctccagatcc gggccgcagg ggcattccgc cgaaattggg
     8401 tcagacagga atatttccat ttaattatcc tcagtgcgcc aggtagtgtt ctggttcgaa
     8461 aatcatgccc gtcaccgggt tgtcgtgcat ggctttaccc gcccatgccc gtcaccgggt
     8521 tgtcgtgcat ggctttaccc gcccatgcac gacaacccag ttggtacgga ccaccggtaa
     8581 cagccggagg ggcggcgtgg ggctttagct gtagctcaat ttcccagcag tattcataac
     8641 cactaaaggc gcggaccagt tctgtcagga cgggcaggtt attgctgcca ggcagaaaac
     8701 gtcggtaatc ctccagcgtc agcgggccga taaccagccg gaacttatac tgcatgtccg
     8761 gcacggcctg gccgatgagt gcaccattgc caataacgga agagggggca ggcatcccca
     8821 gccgggtgat ctcatcgtcc gccacggcta tccagtgcag gacatactcc tgtatccgga
     8881 aaggtacacc gaaatagtgc gccagcgtgg tggccagacc gtccggattg cgggactcac
     8941 gcaccagatg ggccgatgcc gccagacgag cgtgatctgg caacgggctg tcagcgctct
     9001 cccgcaaatc ctgcccgctg aggctggcga cataaaaggc aaagcggtca tgttgcggtt
     9061 tatccagacc gcccccagcg gactgggcgc tgcgccatgc ctggtaaaac tgcgtcagcc
     9121 agcggtgatg aaaaagatca gaaaaacggg tcagcgtcgg atcgcgtcgg ctttcagtcc
     9181 ggttacgcgc cagttcggtg tagtgcagcg gcagtggacc gttcggcccc cacagcccga
     9241 ggctgtacag actgatgtgc aggcgccctt cctgccagcc cacactggcg atttctcgtg
     9301 gcgcaaaggt cagcgcgggc gtttgtccga ggcgaaattt ttccatccgt ggttgctagg
     9361 tgttgcccgc cggaacggta cacaactgca catccacgcg gcgcatcagg ttcagaaaac
     9421 cgtaacgcca ggggggttcc tgcgcctgag tgaaatcgtt gctcatacgc ttccccgctg
     9481 tcccgccctg acaggccagg tcataatttt gccgcgctgc atgctgtgca gcgtcatctg
     9541 cgagaaggta ttaatggaaa catggcgggc gatatagtgt tccagcacca ggccgaacag
     9601 atacggactg atgccggaaa aaccttcttc atccaccgtc agttcacaac tgacgccacg
     9661 cccatagacc agtagtccgg aaccgggcag acggcgggtg accggggtcg ttttacaacc
     9721 aatcaggctg cgcacctggc gcgattgcgg actgtcatga gcaggaataa acaggttcag
     9781 cagatctcgc aacgcctggc cgccggtacg gtgatccaga tcggccagcg gcagataatt
     9841 aaaagataac tggcggatca accgccatgc catctcacgt tcagccagcg ggggctgcgg
     9901 cgagcgtggc gggcggataa gccctacccc cgccaccggg atggctgcat ccacggttag
     9961 atcgtcccgg ccattgcggg caataagaca gggcagatcg cggttagtga ccatggccgt
    10021 gatggtgatg tggcgcagat tttccgggta cggcgcttca tactggtcaa ccagcgacag
    10081 gaacacttcc gagcccgtat agggggtacg ggtgccgtaa cggcgggcgt tttctgataa
    10141 gcggcggggc tcccggcgca gggaaaaata acgtccgtga ttgccttcat cattattgcg
    10201 ggtgtggtag agcgggcgaa aggccatctt ccgtgtggtg tcggtttcca gcccttccac
    10261 ttcttggacc gagaacacct cataatccag cggatgggtt ctgtccacca ccagatgctg
    10321 ttccgtcgtg ctgtgcgtga catcgatccg ggcggtgacg cggggaaaca ggttgataac
    10381 cggggtacag aacaggctga actgtgccgc atttgtctgg tggatcagcc agtccggcgg
    10441 caggcggttc agcaggataa caatctccgc cacgccaccg tcaattttct gtaatccggc
    10501 agacaggcct gtcggggtga aaaaatagaa gcgttccgga caggcaaaat attcatgcag
    10561 caggttatga ccgtgaaaca cgttccacgc cagcggcagc agcccctgat ccggctccag
    10621 gccttcgtac ataacgggct gttgcagatt cacatccagc gcgccgtcaa aatgccccgg
    10681 aatgccagcc agggtcgcga tggcgctggt atgtagaagt tctaacaggt gtgaggccgt
    10741 acgctcctcg ccgcagagat aaaacggcag cctgtccagt ccggcaagct gactgaacgt
    10801 tagttcacca aaggtgcgca gggtaatacg cagcgccccg gcgacgtgaa tgttggccgg
    10861 caggtagcgg tgcagggcag gcatatccgg tggcgcggcg gtcagtcgcg cctcttcgat
    10921 ggcgagcggc cacagcgtta catcctggct gctgcgaaac tggcaggcag tgctttcgcc
    10981 ttccggaatg gcggagaaaa aggctgtgtc gcgcgggacg gtaaatcctt tcgccagatc
    11041 gccttcttcc gtatcgggat gcaactgcgc cacgctcatt gagggcgtgg gtgtgacgta
    11101 attggggctg accacttcca gcaggcgctg ggtgaaacgg gggaattcag cgtcaatttt
    11161 tagctgcgtg cgggcggtca ggaagctgaa ggcctcgacc attcgctcta catacggatc
    11221 ggcaatatcc gttccctgca tgcccagccg ggcggcgact ttgggatgac gggcggcaaa
    11281 ttcggctccc gtttcccgca gatagctcag ttcgcggttg tagtattcca gtagccgtgg
    11341 atccatcggt ctctctctga attaaaaaaa cgtaatgcga ctctgctcca tatccagcgc
    11401 gctacgtacc cggaatgcag tcggatacgg ctgcgtgagg atctcgccgc gaatttcaaa
    11461 ctgtaatgtg ttatggccac tctggcggcg ctcatcctga agtgggatga tttgcagggt
    11521 ggacgctttc agccggggtt caaaccgggt gatggttcgc aaaatggccc tgcggatatc
    11581 gtcccacttg tgctcataca gaaagctgcc cgccagtggc ggcaggccgt agttaagtac
    11641 ggacgctgcc gcgtacggat aacgtgcagc atcaatatca tcttcatggc tgacggtgtt
    11701 gagcagaaat gacaaatccc ggcggatgat ctccttaatc tgaaccgggc tgacgctgat
    11761 accgtggtcg cgtttctggt ttggggcgtc gtcacacagg cggtcaaaca gcgtgggcag
    11821 caggtgattg cctggacgct ggcgggaagc gctcacgcgt cgcttccttc agcggtgtta
    11881 aaggtacagg tggacaaatc cagcaggctg atatcgccgt gaccggtgag ccacaccctc
    11941 tgacccagcg cctgtaccag tgtctctccc ggaccgtcct gccagaccgt ttcccggcac
    12001 agacgcaggg agtcggcagc cttttcagag ccgctgtagc ggggaaacag ccaggcactg
    12061 tgtgtatcgc cattgttcag ggtgacgttg acgggtttcc agatcaaatc gatcagatgc
    12121 gcaggccgcg gcgatttcag cgaacagatt tgtgaaaacg gcagccagat atatgctccg
    12181 cccgcgatca gttcgagtac cggaccggta cgggagtcgc tgtcgctaat ccagtcaaat
    12241 gcgccgccat tccactgccc ggaagtgtct gttatggcct caagcgcgcg gctgcggtga
    12301 gcatcagctt cctgcgcttc accacgggcg ttacaggcga gggcggtcag cagatcctcc
    12361 atccaggcgg gcggcgggag aataacaccg ggccgctgtt cgccctgaaa gcaggcgtgg
    12421 cggtatatct cacagcggat cagctcgcca aatacctgcg cttcgcgagt ataatttgcg
    12481 tccatccggg cgcatagctg gatctgttgc agtgcccgcg accagtcgcc tgccacgcac
    12541 aataactgaa acaggctgtg gcggcgggag gcctgcgctg ggtgcatcct gacctgttgc
    12601 tctgccatct gaatgccctc caccacggaa taatcccgca gcagcgccga caaggtaatc
    12661 ggtagctcgt tattgtttgt catgatatat ctctccgtgt tgtgccgggt ttatccggta
    12721 atggctgtca atgccaatga tgcgatgctc tttccgtgtc agatctggca gtacggtgtt
    12781 atgcgctgtt tccggggcgt attccgggga aagtaaatgc aggatatcag ggggagtctt
    12841 ttcgacaggc cagatagttt catcttcgcc agtggcatcc agtacattga gaatagcgtc
    12901 aatgcttaac ccaccagtga ccatatcctg caatgtctcg gcttcggtga ctaggtcacg
    12961 caatgaatcc agatcctgcg aggagggctc ttcgttaaaa ggaaaaggat cagcatggcg
    13021 attaagatct tgtccgggag ataacaacgc ctcgcggtat tcctgataaa gtcgggtcag
    13081 ggcatttcct ttttccgcac cttctccggt ggaggaggct ccatgaacag gaatgatatc
    13141 gaatggattt tgtgatgtca gatgctgctg cctgaaccaa tccagatcca gatattcggt
    13201 tattgcttcc gacgtgatgg cagtctgcga tatcgttttt actgtgctca ggtccggaga
    13261 tgatggattg ccatgtacaa ggcactatga agataacccc cattctatca ggtcgccttc
    13321 attcagacgc atacggtgat gaggttccac aatctggtcg tttaccgtac agcaatattc
    13381 ggtagactga ttgataatcc accatgcttc ttcgtggcgg gtcagagtga gggcaatctt
    13441 tacatcatca ttcaccttaa aaacaggaat ccagggagag gtaacggtga agataatttg
    13501 tgttccttca gtcgcgtaaa ttgtgttatc ggcgatcccg tgcgcctgta ttttttggag
    13561 tccccacgta atatttggtt tcatt
//
LOCUS       DADLSA010000102        13468 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.102, whole genome shotgun sequence.
ACCESSION   DADLSA010000102 DADLSA010000000
VERSION     DADLSA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.102"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..1272)
                     /locus_tag="JN691_002928"
     CDS             complement(<1..1272)
                     /locus_tag="JN691_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7244326.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQSTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGVPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFE"
     gene            complement(1297..2333)
                     /gene="ypdE"
                     /locus_tag="JN691_002929"
                     /pseudo
     CDS             complement(1297..2333)
                     /gene="ypdE"
                     /locus_tag="JN691_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(2333..3416)
                     /gene="ypdF"
                     /locus_tag="JN691_002930"
                     /pseudo
     CDS             complement(2333..3416)
                     /gene="ypdF"
                     /locus_tag="JN691_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(3431..4678)
                     /locus_tag="JN691_002931"
     CDS             complement(3431..4678)
                     /locus_tag="JN691_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY7244327.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(4700..5026)
                     /locus_tag="JN691_002932"
     CDS             complement(4700..5026)
                     /locus_tag="JN691_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAY7244328.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(5245..6210)
                     /gene="glk"
                     /locus_tag="JN691_002933"
     CDS             complement(5245..6210)
                     /gene="glk"
                     /locus_tag="JN691_002933"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAY7244329.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            6414..7670
                     /locus_tag="JN691_002934"
     CDS             6414..7670
                     /locus_tag="JN691_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAY7244330.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            7785..8111
                     /locus_tag="JN691_002935"
     CDS             7785..8111
                     /locus_tag="JN691_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY7244331.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(8252..9490)
                     /gene="mntH"
                     /locus_tag="JN691_002936"
     CDS             complement(8252..9490)
                     /gene="mntH"
                     /locus_tag="JN691_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAY7244332.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            9826..11028
                     /gene="nupC"
                     /locus_tag="JN691_002937"
     CDS             9826..11028
                     /gene="nupC"
                     /locus_tag="JN691_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAY7244333.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(11078..13267)
                     /locus_tag="JN691_002938"
     CDS             complement(11078..13267)
                     /locus_tag="JN691_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HAY7244334.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGNLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
BASE COUNT         3331 a         3675 c         3339 g         3123 t
ORIGIN      
        1 ttcaaattgc tcctgctcgt ccggcgcgct gtcgcgatcc atatagagca tttcggtacg
       61 gaacagcccg acaccttccg cgccgttggc aaacgcgcct ggcgcttcca gagcggtgcc
      121 gatattcgcc gcaatatcaa tacgcttgtt atcacgggaa taggccagct gcgcggcagc
      181 ctgcgcctgt tgtttttggc gtttatccgc cagcgtctgc gcgacctgat aataaccgct
      241 caccgcgtca ttcgggttaa tcgccagcac gccgcactgg gcgtcaagca ctgcaggttg
      301 cccggcataa cgggcaatcg catccagcgg cacgccactc agtaccggga tcgccgaggc
      361 acgggccaga atcagtgtat gcgaggtgcg cccggttttc tccagaatca tacccgcaag
      421 atttttcaaa tcaaggctca aaaactggct tggggttaaa tcttcagcca ccagaatggt
      481 cggtttttcc agcaccagat tgttgcgcgg cttcagttcc ggccaggtga tatgcagcaa
      541 ctgttcgctg atatcgcgaa tgtcgctgac ccgctcgcgc agataatcgc tggcagaagc
      601 agatagtttg gcgcaaacct gctccatatt gctgatgatc gccgccccca gtccctgatg
      661 ctgttctgtc atcaggcgac ggatattgcc tgcaaattca tcatcctgaa tcagcgacaa
      721 atgagcgctg aggatagttt tgctttcgcc gtcacgctca cgcaattgct ggttcagttg
      781 ctcggcaagc gttgccaggc tgtgctccag ccgggtggaa tcttgcgcac tggcggggat
      841 tgcccgataa ctgtcgaggc tgtcgctttg taacagggtc agcgtaccca cgccgacgcc
      901 gcttgccagc acattgccgt acagtaaatc cgggttaagg cggcttaatg aacgcggcag
      961 cggatgcgcc gtcagttctg cctgcgtcga ctgaacgctg tcgctgtcga taaagcgcac
     1021 caggatgtac tcttccagca cgcgccgcgc ctgctcttca tcgctgccgc taatgttcag
     1081 gctgcaactg tcattaaata aggtgccagt tccaatcaac gccagcgagc ttttagcatc
     1141 ggcctttgcg ttctggcgat ggttgataaa ggtgatttcg ctttgccact ggctgcactg
     1201 ttctttaagt tcccacgccg gacgagcatg tagaccgtta ggcaaaggac agagaaattg
     1261 aatcgttaac ataaccgctc cgggttaatc aggagatcat ctgaaatccg tcagttgaac
     1321 aaccgtctca cgcgtaagac gttgaataag ggcagataaa agttgctcca tctgcaaaat
     1381 gtcgcggcaa tcggcaatcg atgccgcgca atgtccgtgg cgggtggcag gccccattac
     1441 cactgtgggt acgccagtac cggttaagtg caccgctcca ccgtccgtgc cgccgttact
     1501 gaacatatcc gcctgcaacg gaacgccaat ttctgccgcc acggtttcaa tccacgcggt
     1561 aagttttggc ggcgcaatca gcgatttatc gcttaacacc agcatcagcc cgttaccaat
     1621 ctgacgatgg ttagccgcgc cataatcaaa gtttttcgcc cagcaggcgg tatcaagcac
     1681 aatggcgacg tccggcgaca ccgcgcgggt agcagtttgc ccgccgcgta atcccacctc
     1741 ttcgctggaa cttgccacca gccacacttc cgcaggcagt tcggcgtcgt gcagctcacg
     1801 tagtaacgtc accagcagat agcaaccgag gcgatcatca aaggcttgcc catcacccgc
     1861 tggtgaggga ggacctgaaa agtggtatcg aaggtgacgc gatcgcctgg acgaattccc
     1921 gcctgcatca cttcgtcata ggtgcgcgcg ccaatgtcca cgcgcatggc gctaacgtca
     1981 ttcccctgcc gttcgccgtc aagcaggcct ggaattttgc gctcttcacg ggtggtgatg
     2041 cgcaccggct gcagctggcg ggcagccatg cgcacgttgc caaccggcag cacgtcaata
     2101 gcgccttcgc gggagatgct gcgcaccata aatcccactt cgtccatatg ggcgcagatc
     2161 atcaccttcg gacctgtcga ttcattgagg cggatcagca ccgatcccag accatcaaat
     2221 cgcacttctt tttgcaggcg agccgcttct tccagcagga tctgccgcac ttcctgttcc
     2281 gaggaggcga tcgcatctgc ctcgctcaac gcttttaata gcgataaatc cattatgcct
     2341 ctcccgtgag caacactgtt ttcggcatgg cgtagagcac ttctgcgcct tgcggggtaa
     2401 ccagcacaac atcttcgatg cgcacgcccc cttgccctag caaataaatc cccggctcca
     2461 cggtcagcaa catgcctggc tgtagcgtcg tggtgtcccg cggtgaaaaa cgcggatctt
     2521 catggacttc aatgccgata gcgtgaccag tgttatgacc gaaatagtcg ccaaatccag
     2581 cctcggtaat gacccggcgc gcggcgtcgt caatctgctg gcagcgcacg ccggggcgaa
     2641 ttgcagagat tgccgcgagc tgtgcctgca ggacaatctg atagacgtca aacagcgggt
     2701 gagattcggc gctcacccct tcgccattca ccagcaaggt gcgcgtcata tcagcagtag
     2761 ccctgataca gcgcaccaaa atcgagagtg acaaactcgc ccgctgcaac aatcttgtcg
     2821 ctggctttgc cgtgcggcag cgccccgcgc cagccgctgg cgacaatggt gtcaaaagag
     2881 gctttttctg cgccctgctg gcgcataaac cactccagtt cagcggctat ctcgcgctcg
     2941 ctcatccccg cctgaataaa gcggcgaata tgctccgctc cgcgatcggc aatcccacag
     3001 gcgaggcgga ttatctccac ctcctctggc gttttgattt gccgcagcac atccggcgtg
     3061 gcgctaacca gtttcgcatt gagttcagac ttccagcggt gcgcggtttc ccaactcacc
     3121 tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg caatgatttg attgacgata
     3181 gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt agccttgcgc acgggcttcc
     3241 acctcaacga aatagcgcga atctaccaga atgtgcgcac tttcacggct aataaccaca
     3301 tagcctgatc cagtggagat ccccagatgc ggctgtttgt tctgccgtga ggagagaagc
     3361 accgcatcca gttgttgcac cttaagccag tcgcgcagcg aagcgagtaa tgtcatctgg
     3421 attttccttc ttacaggcta tcgattaaca atttgccttt gcggaacatc atcagacgca
     3481 ggaacaccac catcagcgca gtaatgattg cccccagcgc gatccccgcc atataaacgc
     3541 ccaggttagt gaccagcggc catgcccaga tagcggattc cgggaaccat tgcactgcgc
     3601 ccagccagac agctgcggtt gagccgacaa tcgcgccgac catatatgac ggaatggcgg
     3661 tgatggggct ttccagcgca aacggaatcg caccctcact aatccccata aaggccagga
     3721 acatcgcggt cttaccctgc ggataaagtt gagcgttgaa caggcgtttg ccggttaaac
     3781 gacggtcaat aatggtcgcc agacccagac caatcggcgg aataacgata gcgatagaac
     3841 gagcggtgac cggtaatacg tggtcagtgg taaagctgaa ggcaacgaaa cctgcggctt
     3901 tgttgatcgg gccaccaagg tcgattgctg tcgccgcggc gatccccatt gcgtacatca
     3961 gcgcgccctt ctcacctgcg gcagtcagca cagtacggat accgccgttg atccagccac
     4021 caaagggggt gatgacgtag tacatcgcca gcatgacaaa aatggcggaa agaatcggta
     4081 gcaaaaatgt ggttttgaac gctaacagga aatccggtaa ctggattttc tggttcatcc
     4141 atttcacaag gtaaccggca acgatagaaa taatcagcgc accaatgaag gtggacggca
     4201 ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa gttcagcagt tgcgtcggct
     4261 gggtagacat caacccgccg ataaaaccag ccgggaacgc cagtttgcca ccgatagagt
     4321 tcgccacaaa tgcagcaaac atcggaatgg caaaaccaaa caacacgccg ccgaaggact
     4381 gcgacagcca ggcaaattta agtaaggaga ggtcgaaacc ggagaatttc ccgctattaa
     4441 gagcatccat gatgccgatc tcagcaggaa ttttaagcca gctataagca atcagctggc
     4501 tgaaagcgag gatcacaccg cccataatta aggtcggcac catgcgtgaa atccctgaca
     4561 tcacatgctg cgggagttca ccccagaagc tgcttttaga ggcctgcgga ttcttaactg
     4621 ccgctgccgc accggatgcg ccaggcacaa cggttgcact gcgtttttta atggccataa
     4681 caattatccc tgtcgaatat tactgttgtt cagaggcaat catctcttcg atttctttga
     4741 tgatgcccgc agcgttttta attgcgtcct gcaaagtgat ttcataaacg tcgcgtgatt
     4801 cgaaacgttc gttatcttcc ggggtaactg ccacggaatg gatgatgatg gtcgcttcgg
     4861 cgatatcctg cgccgtcagg cgattctgga tgccgtccgc gccctgggtt tcaattttca
     4921 cttcataacc ggcttctacc gccgcttctt ccagcgcctg agcggccata aaagtgtgag
     4981 ccaggcccat tgggcaggca cataaggcaa tcagtttctt actcatcgtt aattcctcgt
     5041 tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac aaatcgccgt taccagacaa
     5101 ataatgcatt tactggagaa ttaatcccgc tgtacggaag tgtgacgcag gtcggccttg
     5161 tgaggggaca caaagaaaaa ttgaattaac tgtatgattt aaaagattat cgggagagtt
     5221 acctcccgat ataacaggaa ggatttacag aatgtgacct aaggtctggc gtaaatgtgc
     5281 accggaaccg agaagacccg gatagtcatg gacgatgaga tacaccggaa tatcatggac
     5341 atattcttta aagcgccctt tatcttcaaa tgcggcacgg aaaccggagg ctttgaagaa
     5401 ctcaaggaag cgcggcacga taccgcccgc aataaacacg ccgccaaatg tcccgagatt
     5461 gagcgccaga ttgccgccaa aacggcccat aatgacgcaa aacagcgaca atgcgcggcg
     5521 gcaatcggtg cagctgtcag ccagcgcgcg ttcggtaata tcttttggct tgagattttc
     5581 tggcaggcgg ttgtcagctt tcacaattgc gcgatacaaa ttcaccagcc cagggccaga
     5641 aagcacgcgc tccgccgaaa catgaccaat ttccgcacgc aatatttcga ggataatggc
     5701 ctcttcttca ctattcggcg caaaatcaac gtgaccgcct tcgcccggca agcttaccca
     5761 acgcttatcg acatggacca gatgcgcaac cccaagcccc gttccggcac cgtaaaccgc
     5821 aataggctta ccttcgaccg gttctgcgcc accaaactga atcagatgct cttttttcag
     5881 catcgggatc gccatcgata cagcggtaaa atcgttaata atttccagat ggctaaaacc
     5941 gagattcttt ttcatttcgg caattgagaa cgcccaggta tggttggtca ttgccaccca
     6001 gtcaccggta attgggcaag cgatggcaat acagccgtct ttcacctcga ccttatgttc
     6061 ttcaagataa acgcgaatga ccgcttcgag gctggggtaa tcaagccctg aataggtctt
     6121 agcctgcgag atttcaccac tggcaatatc acacagagca agacgtgcgt tggtgccgcc
     6181 cacatcaccg actaatgcat actttgtcat tcttcaactg ctccgctaaa gtcaaaataa
     6241 ttctttctca cactgtaaat acctgggggc ataacaacaa cgccggaaag gcaggctccc
     6301 tgtaaatatc gatctgggtc acacaattac tttatcgttt cagcaccaat tgcagcgatg
     6361 cctttttgca agctgggcaa actaagtatc tgaccccgca taaggaatag aacatgctcc
     6421 atccgcgagc cagaaccatg ttgttattat cgctccccgc cgtggcaatt gggattgcgt
     6481 ccagtcttat tctgattgtg gtgatgaaaa tcgcctcggt attacagaat ttgctctggc
     6541 aacgactgcc gggaactctg gggatagccc aggattcacc cctctggatc atcggtgtat
     6601 taacgctaac gggtattgcg gtggggttgg ttatccgttt cagccagggt catgccggac
     6661 cagaccccgc ctgtgaaccg ctgatcggcg caccggttcc gccctctgcg ctacctggac
     6721 ttatcgtagc attaattctc ggtcttgctg gcggcgtcag cctggggccg gaacatccga
     6781 tcatgaccgt caatatcgcc cttgccgtcg ccattggcgc tcgtctgtta ccgcgcgtca
     6841 accgaatgga gtggactatt ttagcctctg ccggaaccat cggcgcgctg tttggtacac
     6901 ctgttgcggc ggcgttgata ttttcgcaaa ccttaaatgg cagtagtgaa gttccgctat
     6961 gggatcgtct ttttgcgccg ttaatggcgg cagcagctgg tgcacttact accggattat
     7021 ttttccatcc ccatttttca ctgcccattg cgcattacgg acagatggaa atgaccgata
     7081 ttctcagcgg tgcaattgtc gcggcgattg ccatcgcagc agggatggtc gccgtatggt
     7141 gcttaccacg gttgcacgcg atgatgaatc aaatgaaaaa tccggtgctc gtgctgggta
     7201 ttggcggatt tattctcgga attctggggg ttattggtgg accagtttcg ctgtttaaag
     7261 ggctggatga gatgcagcag atggtggcaa atcaggcttt cagcaccagc gattactttt
     7321 tgctggcggt aattaaactt gccgccctgg tcgttgctgc cgccagtggc tttcgcggtg
     7381 ggcgaatctt cccggcagtg tttgtcggcg tggcattagg gttgatgctg catgagcacg
     7441 ttcccgccgt tccagcggca ataaccgttt cctgcgctat tctcggcatc gtgctggtgg
     7501 taacacgcga tggctggtta agtcttttta tggcggcagt cgttgtaccc aataccacat
     7561 tgctaccgct gctctgtatc gtcatgcttc cggcatggct gttattagca ggtaagccga
     7621 tgatgatggt caatcgtccg aagcaacagc caccccacga taatgtttag caaaaaatgc
     7681 tctcctttat gatttaagag ttatggcgat gatacggggc tttacctccc cgtaatattg
     7741 cgttaacagg ccgctgacgg cttcgatcgt gaaggagaat aacaatgttc aggtcactgt
     7801 ttctggcggc cgccctgatg gcatttaccc cgcttgcagc aaatgcaggt gaaatcaccc
     7861 tactgccatc gatcaaatta caaattggcg atcgcgatca ttacggtaat tactgggacg
     7921 gtggtcactg gcgagatcgt gattactggc atcgcaatta tgaatggcgc aaaaaccgct
     7981 ggtggcgtca tgataatggc tatcatcgtg gctgggataa gcgtaaagca tatgagcgtg
     8041 gctatcgtga aggctggcgc gatcgtgacg atcatcgcgg aaaaggccgc ggacatgggc
     8101 accgccatta aacgcgtcct ccaatggagc acaatgcctg atgcgacgct agcgcgtctt
     8161 atcaggccta caaaccggct ctttaacgcg tagggcggat aaggcgttta cgccgcatcc
     8221 gccaatagtg ccagatgcga cgctcattca actacaatcc cagcgccgtc cccaccaaca
     8281 accagatatt cagcgccacg accagcacta caatcaccca gcctgtctgt tttacgcgtt
     8341 tgctgttcac cagatcgccc atcaacttgc tgtcactggt gaaaatcagc agaggaacca
     8401 gcgccagggc gataccaaaa cttaacagca cctgactcat aaccagaatc cgtgtcggat
     8461 ctaatcccat cagaatgaca ataaatgacg gcaacatggt gactgtacga cgcacccaca
     8521 gcgggatatg gaagcgaatg aatccctgca tcaccacctg ccccgccagt gtccccacca
     8581 ccgttgagga cagtccggca gcaaccagac ttaacccaaa gaccgttgcc gcagcatggc
     8641 ttaacagcgg ttgcagcgtc agataagcct catcaagatc ggcaacacca gtatgaccag
     8701 aaaagtggaa cgccgccgca gctgtagcca tcatcgccag attgacaaaa ccggcaatcg
     8761 tcatggcgat agccacatcc catttggtgg cggaataacg ttgttgacgc gaaccgccat
     8821 gtaaatgctg agtgagcgaa gagtgcaaat aaatcacatg cggcataatc gtcgccccta
     8881 acacgcctgc tgccaggaag accgcctccg aagtaggtaa actcgggatc accattcctt
     8941 tacccagctg cgccaggtta ggctgggaga aaatcaactc gacaatgtaa gccgcggcaa
     9001 caaacaacag taacccgcca atcactttct ccagcggttt ttgcccgcga cgttgcagca
     9061 ttaaaatcag gaaagtcgcg atccccgtca gcaccgcgcc ctgcaacaac gaaacaccaa
     9121 gaatgagttt aaaaccgatc gccgcaccaa taaattccgc caggtcggtt gccatcgcaa
     9181 taatttctgc ctgaacccaa tagaaccaca ctacgggacg cggatagtga tcacgaatct
     9241 gctccgccag atttttaccg gtggcaatcc ctagtttggc agagaggatc tgaatcagca
     9301 tcgccatcag gttggcccaa acgacaaccc acagtagctg atagccgaag ctagcacccg
     9361 cctgaatatt ggtcgcaaag ttaccgggat cgatataacc aatcgccgca atgaacgcag
     9421 gtcccattaa tgcgagcctc atcttgcgcg ccgcccgtcc gctgctactc tcaacgcgat
     9481 agttcgtcat cttgtgcctc taaaacatag cctttgctat gtttcatgcc atgccaaacg
     9541 agaatgatta tcaaattcat ttaaatggat tgtggtgatt tctctgatag accaggatta
     9601 tgattacgaa aagattgatg acttgaatgt tatggttatg tttaatgtta gcacatttac
     9661 ataactttca gcttccatac acaacatagc agaaatgtat gacagatcac tatttttgaa
     9721 gcctgtcaca ggacgtcatt atagtgtgtg tcagatctcg ttttccttaa ccatgttaca
     9781 tagaatgtgc acggaaattt aacctgcctc atatttggag caaatatgga ccgtgtcctt
     9841 cattttgtac tggcacttgc cgttgttgct attctcgcac tgctggtaag cagcgaccgc
     9901 aaaaaaattc gtatccgtta tgttattcaa ctgcttgtta tcgaagtgtt actggcgtgg
     9961 ttcttcctga actccgacgt tggtttaggc ttcgtgaaag gcttctccga aatgttcgaa
    10021 aaactgctcg gatttgccaa cgaagggact aacttcgtct ttggtagcat gaatgatcaa
    10081 ggcctggcat tcttcttcct gaaagtgctg tgcccaatcg tctttatctc tgcactgatc
    10141 ggtattctcc agcacattcg cgtgttgccg gtgattatcc gcgcaattgg tttcctgctc
    10201 tccaaagtca acggtatggg caaactggaa tcctttaacg ccgtcagctc cctgattctg
    10261 ggtcagtctg aaaactttat tgcctataaa gatatcctcg gcaaaatctc ccgtaatcgt
    10321 atgtacacca tggctgccac ggcaatgtcc accgtgtcga tgtccatcgt tggtgcatac
    10381 atgaccatgc tggaaccgaa atacgtcgtt gctgcgctgg tactgaacat gttcagcacc
    10441 tttatcgtgc tgtcgctgat caatccttac cgtgttgatg ccagtgaaga aaacatccag
    10501 atgtccaacc tgcacgaagg tcagagcttc ttcgaaatgc tgggtgaata cattctggca
    10561 ggtttcaaag ttgccattat cgttgccgcg atgctgatcg gctttatcgc cctgatcgcc
    10621 gcgctgaacg cactgtttgc caccgttact ggctggtttg gctacagcat ctccttccag
    10681 ggcatcctgg gctacatctt ctatccgatt gcatgggtga tgggtgttcc ttccagtgaa
    10741 gcactgcaag tgggcagtat catggcgacc aaactggttt ccaacgagtt cgttgcgatg
    10801 atggatctgc agaaaattgc ttccacgctc tctccgcgtg ctgaaggcat catctctgtg
    10861 ttcctggttt ccttcgctaa cttctcttca atcgggatta tcgcaggtgc agttaaaggc
    10921 ctgaatgaag agcaaggtaa cgtggtttct cgcttcggtc tgaaactggt ttacggctct
    10981 accctggtga gtgtgctgtc tgcgtcaatc gcagcactgg tgctgtaaaa ccatacataa
    11041 aaaagccggg gatcattccc cggctttttt acgcctgtta atcagctaat ggctgcgggc
    11101 gaccaatcaa atacccttgc agatattgca ccccgagctt atgcaatagc gcctgctgct
    11161 gttgcgtctc gacaaactcc gcgaccacac tcaatgactt cgcttttgcc agatcggtaa
    11221 ttgatctcac aatcatcgca tccagcgtat tcgtgacaat atctttcaca aagacgccat
    11281 caattttgat gatatcagcc tgcaaacgct ttaaccgttc gtagttggca tatccggtgc
    11341 caaagtcatc aatcgcaatc cggaaaccaa acttatgcag ctgctcgatg ttgtacatgc
    11401 tgctttctgc gttagaaaac gcctgctcct cggtgatctc aagaatgacc gcctgcgggg
    11461 agatgtgata acgcttaaac agacgaataa tccgcccggc aatattcttt tgcagcagcg
    11521 tgagcggcat taaattgact gaaaagcgcg gtcctttttt gtcgcaaggg tgtgttgcca
    11581 gccacttcaa caaggattcc agcacttgca aatcaaaacg cgcgctaagg ttaaactgag
    11641 caataagggg cagaaactta tccggggtca taatgccgcc gtcatatttc agtcgcgcga
    11701 ggatctcatc ataaccttca ccctctttgt tgcgaattgg ctgggcgtag agcagcaaat
    11761 caccctgatc taacgccgtg cgaatggtat tcagcaatag cacctgttta gtggtctgcc
    11821 cggaaaccat ctcctctctg ctatccagcg ccagcacatg atgatgtgcg caggattgct
    11881 ccgccagcca gcttaactgc cccaacaagg gttgcagggt ttcctgattt ccatcaaaac
    11941 gcccccaggc agcaccatag cccatatcca gcccggtatt gttccagtga atttgccgac
    12001 tattcaggat gttaaccata tgctggagtc gcccttccgt ttccggcccg ctcagcacta
    12061 acagcagttc actacccggc aattgataca acttttcgtt ttcctgcatc aacggcagca
    12121 gcgtacggca aattgagcgg atacagtgaa cgcgcatcat taagccgtaa tgacgactca
    12181 taaactcaag attatcaatg cgcaggcagc aaaaactctt gcccgcctct tgctccggcg
    12241 cttgttccaa cgcacgaaag ttgggtagga gcgttaacgg atctgtcagc gcctgcaaat
    12301 gccagcggcg attaagccat tcactgcgat gataaatgcg caccatatag agcaggcaaa
    12361 cgctaaagga aatcagcacc gcgagaataa atgccagcga atattcggtt tccacccctt
    12421 gcaaaaagtt ctggttgtaa ttcagaaggc aaagcgttga aacagcccag gtgagattta
    12481 aaaacggata gcgaagatta ccgaccccga gggtaaagat gatgaagaaa acgggtacca
    12541 ggtaaccggc aataaagtcg ttttcataag gtgtgcacaa cagaagtagc agcacgctta
    12601 acgctgccag ccagcttaag gtaaatgcgc gtttctcttt gcccaacgac ggagcgatat
    12661 ccctgcgcca caatatctgc gcaaagtggg gacttacaat catgcgagtg agatagtaga
    12721 aaagcatgtt gtaaatcagc actgcggtga aaaggcttag taaatcaacg accgtgaaaa
    12781 tggcatccgc atcgccgaaa aaggtggata tctttagcgg gaaatcaaag aagctcccca
    12841 caagatacat gctgcatttg atgccgatcg gcgtcaccaa accaagccag aacagacgtt
    12901 gccagacata tcggctggtc aatccataac gccaacgcgt ccccagctgc cagcgtaata
    12961 ttgcgcaggc acacaacacg acaaacgtct ggctgccgag catgaccgca gtctgtaaaa
    13021 aagagagatg aaagttccac agattggtgc aaaacatccc gcataaaacg ggaacgacac
    13081 cgcgccagcc aaagataaac aagattgaca gcattacgca cagtggcatc cacgccagaa
    13141 agatataact ggaatggata atggcaagtg gcgaaataaa acgggatagc tgaataagta
    13201 ccacggtgag cgtaaacgct agtgtgaata tcttgatatt ttttatcagg ttatgctcca
    13261 caaacatgag tacactactc tcactgatcg gggttttcag gcacacaaat aataagttta
    13321 ttcgcatcgc gaagcaaaca ggggccagcc tgttgccgat ttttcatcca atgatgcttt
    13381 caccaggcgg cgctaacccg gacgtagact gtattacaaa agcggcaaaa agcagagaca
    13441 aaaaaccccc gctttgcagc gagggttg
//
LOCUS       DADLSA010000103        13374 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.103, whole genome shotgun sequence.
ACCESSION   DADLSA010000103 DADLSA010000000
VERSION     DADLSA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.103"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            357..1187
                     /locus_tag="JN691_002939"
     CDS             357..1187
                     /locus_tag="JN691_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7244335.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIDIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1328..2101)
                     /gene="uxuR"
                     /locus_tag="JN691_002940"
     CDS             complement(1328..2101)
                     /gene="uxuR"
                     /locus_tag="JN691_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAY7244336.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2316..3776)
                     /locus_tag="JN691_002941"
     CDS             complement(2316..3776)
                     /locus_tag="JN691_002941"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAY7244337.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3857..5041)
                     /gene="uxuA"
                     /locus_tag="JN691_002942"
     CDS             complement(3857..5041)
                     /gene="uxuA"
                     /locus_tag="JN691_002942"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAY7244338.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRLGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5381..6724
                     /gene="gntP"
                     /locus_tag="JN691_002943"
     CDS             5381..6724
                     /gene="gntP"
                     /locus_tag="JN691_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HAY7244339.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMIYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGMLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6975..7877)
                     /gene="fimH"
                     /locus_tag="JN691_002944"
     CDS             complement(6975..7877)
                     /gene="fimH"
                     /locus_tag="JN691_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HAY7244340.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7897..8400)
                     /gene="fimG"
                     /locus_tag="JN691_002945"
     CDS             complement(7897..8400)
                     /gene="fimG"
                     /locus_tag="JN691_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAY7244341.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8413..8943)
                     /gene="fimF"
                     /locus_tag="JN691_002946"
     CDS             complement(8413..8943)
                     /gene="fimF"
                     /locus_tag="JN691_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HAY7244342.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8953..11589)
                     /gene="fimD"
                     /locus_tag="JN691_002947"
                     /pseudo
     CDS             complement(8953..11589)
                     /gene="fimD"
                     /locus_tag="JN691_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
     gene            complement(11656..12381)
                     /gene="fimC"
                     /locus_tag="JN691_002948"
     CDS             complement(11656..12381)
                     /gene="fimC"
                     /locus_tag="JN691_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY7244343.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12418..12957)
                     /locus_tag="JN691_002949"
     CDS             complement(12418..12957)
                     /locus_tag="JN691_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7244344.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(13022..>13374)
                     /locus_tag="JN691_002950"
     CDS             complement(13022..>13374)
                     /locus_tag="JN691_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7244345.1"
                     /translation="QAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAIDATRTDVLA
                     LQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARYYAIGEATP
                     GAANADATFKVQYQ"
BASE COUNT         3284 a         3432 c         3190 g         3468 t
ORIGIN      
        1 tttatttccg cataatcagc tatgtattat ttgaaacaat tagtttgtaa ataatataaa
       61 ttgataacat atcaaataca tcaaaaatac cattcacagg cattattaat atagacttca
      121 atatagcaac atcaaattcc aattagtaat aagatatgct tttaaattgc atgcaaataa
      181 ttttaaatga gcgttttttg atactttaga gtgtttccta ccctttaata tcactgtgta
      241 gagttgatat cactacaaca gaaacatgca tgtcatttgt ttacttctct ttttactaca
      301 gaaaaattta tctatttttc taaacatcag ttaaatactc ataaaaggta actattatgt
      361 ctatgccatt aagcaatgcc gctaataacc aattcaatac aaataatcac ttccttcatc
      421 atccaaaagt cgacagtgag ttaacacgtc gatatgaata tgcacgcctg gatacggaaa
      481 atatctatct tctgcctctt gctcgtggca ataaccataa ttacgatgga aaaagcgttg
      541 tcgaaattag aaaacttaat attggcaaac agtcctggcc atttaattat gtgacagata
      601 catgcagaga attcaatggt attacaacaa caggacgtat gctctatcga aacttgaaaa
      661 tcacctcagc tttagacgaa atatatggtg gtatatgtaa aaaggcgcat gctgctacag
      721 agttagcaga aggtttacgg ctaaatttat ttatgaagag ccctttcgat cctgttgagg
      781 actatacagt tcatgaaata acattaggcc ctggttgcaa tgttcctggt tatgccggaa
      841 ccacgatagg atatatttca acccttccag cttctcaggc aaaaagatgg accaatgagc
      901 agccaagaat tgatatctat attgatcaga taataactgt ctcaggggta gcaaattctt
      961 ccggattcgc ccttgccgca ttacttaatg ctaatattga gatgggaaat gacccaatca
     1021 ttgatataga agcctatcct ggtaccgcgg agatccattc aaaaatggga tataacgtta
     1081 tccccggtga tgaagatgcg ccactcaaac gcatgactct gcaaccgtca tctcttccag
     1141 aactgtttga attaaaaaat ggtgaatgga attatattgg taaatagcca ctgagaagaa
     1201 tgagtactaa tattcaggtt atacagatat ttaaacaaaa ccctgcaatt tacaaagtaa
     1261 tactcattgc gacttcttaa tttaagaaag cgcaaggaac gtttaccctt gcgcttatta
     1321 taataagtca ggcgtcgact ttatccagcg gccatgaatc aaagagatag ccgtcaaaat
     1381 aaatatcgtc aacgttcgag aattccagca gacgttgctt aacgttttcc agatgctgcc
     1441 acattgccag cttcgccgct cgggcatctt ttttgattaa cgcggcgagg atctgtttgt
     1501 gatcgcccaa ccactctttg cgatacaggc tgtcatccag atggctgtgc aactggatcc
     1561 acattggatt gttttcccgc cactgccagg actgacggaa taactccacc agcatgctgt
     1621 tatgcgttgc ttcagcaatg gcgagatgga actgcatgtc accgctttcg ctgctacccg
     1681 gcgcgctgga agccagttca cgctcttcca gttgcaatgc ctgacgcatt ttgacgatat
     1741 cttcgcgggt agcctgcagt gcggcaaact cggcgatgtt actttccagt aactgacgcg
     1801 cctgtaacag ctcaaaagga cccgcatcat tgcagacgtt ggcatccgga ctgtctgtgt
     1861 tgtggctgcc tgagctatca agcacataga taccggcacc ccggcgcact tccaccagcc
     1921 ctttgatctc cagcatgatc agcgcttcac gtaccaccgt ccgcgtgaca tcaagcattt
     1981 ctgcaatttc acgctccggc ggcagccgtt cgccaggatt gtacggcgtc tttatgatca
     2041 gatcgcggat catcgccccg acttcctggt aaggtctttg cgcagaggtg acagatttca
     2101 tggtgttgtg tccgttaggt gcggttaatc aaatttttgc cggatgcggc gtgaacgcct
     2161 tatccggcct acaacgggca attttacggc ccccgttaat ctcaaactat accattagca
     2221 ctggaagttg gcacgtacag gtgggcaaaa aaacgaaaac tggtcaacca aatatcagtt
     2281 tgattgacca gtaagtctgt agggtaatca gttagttaca gcgcagccac acactcgcgc
     2341 gcaccgcgtt cgcacagctg ctgatatgcc gccgtcaccg cgccaacaaa gtcggcattc
     2401 tgcggcagat catcagcaaa aataccgctc aggcccagca gcgctttcac gcggtctgcg
     2461 ccctgatact gcgcgttaat cttctggaac tcggccagca tcgggtcgac cacgtcaatg
     2521 gcattaccct gctcatccac gccctgggtg taacgcatcc agccagccac gcccagcgcc
     2581 aggtgacgcc agctgccgcc gttttgcagg tgcagacgca ccgggtccag tagacgctgc
     2641 ggtaacttct ggctgccgtc catcgcaatc tgccaggtac ggtgacgcag agacgggttg
     2701 ctgaaacgct cgatcagcag cgtcgcatag gcgttcaggt ccgtaccttc cggcatcgac
     2761 agcgttggcg cttgttcctg catcatcagg gcaaaggccg ctttgcgata atccgggtta
     2821 gtcatggtgt cggcaatggt ttcatagccg ccgaggtaac cgaggtacgc caggaaagag
     2881 tggctgccgt tcagcatacg cagcttcatc atttcgaacg gcacaacgtc tgcaacgaac
     2941 tgtgcgccca ctttatccca gtccgggcga ccattaacga agttatcttc aatcacccac
     3001 tgacggaacg gttcgcaggc aatggcgcac gggtcgtaaa cacccagctg gtcagcaatt
     3061 tcctgtaagg tttctggcgt cgccgccgga acgatgcggt caaccatggt gcacgggaag
     3121 gtgacgttct cttcaatcca tgccgctagc tgcgggtcac gcgcctgagc cagccccagt
     3181 accgcgacct tcgccacatg accgttttca cgcacgttat cgcaggacat caccgtaaat
     3241 gctttcagcc ctttttcacg acgcagacgc aaggcttcga cgatgtaacc aatcgcggac
     3301 ttcggcgcag tcgggttttc cagatcgtgc ttgatcagcg ggttattgag atccagttga
     3361 ccgcttgccg catcagcgca gtagcctttt tccgtgaccg ttagagagac aatcgccgtt
     3421 tgcggacgcg ccatcgcgtt gagaatacct tcgcagccgt cgatttccgg atgcagcgct
     3481 tctttcatcg aaccgataat tttcagctcg gtgctttctg cgcctttttc cgctacggtg
     3541 tacagcagtt gctgtttttt caggttttcg atcagcatgc ggtcgttgcc tggcatcagg
     3601 ttaacttcgc agatgcccca gtcgctgtcg gtgctttcca gcagatgatg ggtatacagc
     3661 gcctggtgcg cgcggtgaaa cgccccgcaa ccgagatgca caatgcgtga ttccagacga
     3721 gaatgatccc atgacgggcg ggcaaccggc agattgctgt caacaatagt agtcatcgta
     3781 aactccagaa tccgtcttca gagagtgacg gcaaggaaga aggaaccggc acggcgcgca
     3841 gccatgccgg tgggtattaa cggctaaaga aagcgcgctg gatcgccagt tcgacaccgc
     3901 gaacttcggc caggcctttc agacgaccaa ttgcggagta accagggttg gttttcttct
     3961 tcaggtcgtc cagcatctga tgaccgtggt ccggacgcat cgggatcagg tcttctttgc
     4021 cttccgcttt acgacggtgt tcttcttcaa caatcgcttt caccacttcg tacatatcaa
     4081 cgtcaccgtt caggtgcgcc gcttcgtgga aggttttcgg gttatcttca cgcatggtgg
     4141 agcgcagatg ggtgaagtaa atacgcggac cgaactgctt gatcatatca accagatcgt
     4201 tgtcagcacg cacgccgtag gaaccggtgc acatggtgaa accgttcgcc atgctgttta
     4261 cggtatcaac catccactgc atatcttcaa tggtggaaac aatgcgcggc aagccgagga
     4321 tcgggcgcgg cggatcgtcc gggtgaaccg ccatacgcac gccaacttct tcagcaactg
     4381 gaataatcgc tttcaggaag acggcaaagt tttcgcgcag tttggctttg tcgatatctt
     4441 tgtacagctc caggtgctta cggaactggt cgagggtata cccttcttcc gcgcccggca
     4501 gaccggcaat gatgttacgc gtcagacgcg ctttgtcttc gtcgctcata gtggcgaagc
     4561 gttcagctgc ctgagcaatt tcttcttcgg tgtaatccgc ttccgcgcct agacgtttca
     4621 ggatatgcat ttcgaatgca gcgaattcga tctggtcgaa gcgcagagct ttggagccgt
     4681 ctggcagcac gtattcgagg tcagtacggg tccagtcgag caccggcatg aagttgtagc
     4741 acacggtgcg aatgccacac tgcgccaggt tgcgcagagt ctgctgatag ttagcgatcc
     4801 actgctcata gttgccagtg tgggttttga tatcttcgtg aattggtacg ctttctacga
     4861 cagaccacac caggcctgcg tcttcaacga tcgccttgcg tttgaggatc tcttctacgg
     4921 accatacttc gccgttcggg atatggtgca gcgcggtaac cacgccagtt gcgcccgcct
     4981 gacggacatc ggctaaagaa accggatcgt ttgggccgta ccagcgccag gtctgttcca
     5041 tgatttttcc tctttcacat agtgggttcc acagcaaaga gacgcggaac cttggagttg
     5101 tttaggtatc cggatgaaca accagaccgc agccgtggcg ttgcatcgga tgagatggcg
     5161 tataagttct accttttggt aagacatgtc aacctgaatt ctgaaattgg ttaaccacat
     5221 cacaagaatt tcactctcat ggttgttgct gcaaattgca gcaaagggta atcgatggtt
     5281 attgatcaga ggatgtgaca ttcatcgcaa caatggttga ccaatttaca taacatgtcg
     5341 cccaaaataa caacggttca accaagtaag gtaaaacgtt atgcatgtgc ttaacattct
     5401 ctgggtggta ttcggcattg gcctgatgct gatactgaat ttgaagttca aaatcaattc
     5461 aatggtggct ttgttggtgg cggcgctgtc cgtcgggatg ctggcaggca tggatttgat
     5521 gtcgctgctg cacaccatga aagcgggctt cggcaacacg ctgggagaac tggctatcat
     5581 cgtggtgttc ggtgcggtca tcggtaaatt gatggtcgac tccggcgcgg ctcaccagat
     5641 agcacatacg ctgctggcgc gtctcggtct gcgctatgta cagctgtcgg tgattatcat
     5701 cggccttatt ttcggtctgg cgatgttcta tgaagtggcc tttatcatgt tagcgccgct
     5761 ggttattgtt attgccgccg aagctaaaat tccgttcctg aaactggcga tcccggcagt
     5821 agcagctgcc actaccgcac attcactgtt cccaccgcag ccgggtccgg tggcgctggt
     5881 gaatgcttat ggcgcggata tggggatgat ttatatctat ggcgtactgg tgacgatccc
     5941 aagtgtaatc tgcgcaggtc tgatcctgcc gaagttcctc ggcaatcttg agcgcccaac
     6001 gccatcattc ctgaaagcag atcaaccggt agatatgaat aatctgccct ctttcggcgt
     6061 ttcgattctg gtgccgctga tcccggcgat cattatgatc tctaccacca tcgccaatat
     6121 ctggctggta aaagataccc ctgcctggga agtggttaac tttattggtt cctcgccgat
     6181 tgcgatgttt attgcgatgg tggttgcatt cgtactcttt ggcaccgcgc gtggtcatga
     6241 catgcagtgg gtgatgaacg cttttgaaag cgcggtgaag agtattgcaa tggtgattct
     6301 gatcatcggt gcgggtggca tgctgaagca gaccatcatc gacaccggca ttggcgacac
     6361 catcggcatg ttgatgtccc acggcaatat ctcgccctac attatggcat ggctgatcac
     6421 tgtgctaatt cgtctggcga cgggtcaggg tgtcgtttcg gcgatgaccg ccgccgggat
     6481 tatcagtgct gcaatccttg atccagcaac cggtcagctg gttggcgtga atccggcgct
     6541 gctggtactg gcgacggctg cgggttccaa caccctcacc cacattaatg atgcatcttt
     6601 ctggttgttc aaaggttact ttgacctgtc agtaaaagac acgttgaaaa cctggggtct
     6661 gctggagctg gtcaactccg tggttgggct gattattgtg ctgattatta gcatggtagc
     6721 gtaaaagaac accagagcct gccaatggca ggctcagact gatgactaaa gtctaaaaat
     6781 gtgcccggac agtcccctcg cccctccggg gagagggtta gggtgagggg aacagaccag
     6841 taaagtgcgc tttgtttatg ccagatgcga cgctgacgcg tcttatctgg cctacaaagg
     6901 gctaacgtgc aggttttgta ggtctgatag cgtagcgcct caggtaccag cattagcaat
     6961 gcgctgtaat ttttttattg ataaacaaaa gtcacgccaa taatcgattg cacattccct
     7021 gcagtcacct gccctccggt acgtgcgtaa tttgccgtta atcccagact taccgccgaa
     7081 gttcctactg ctcctaacga taccgtgtta ttcgctggaa taatcgtacc gttgcgcgtc
     7141 aactgtacgc cgacgccctg cgctggtgaa aacgacgcgg tattggtgaa aatcgagttg
     7201 cccgcatctg cggttgtgcc ggagaggtaa taccccaggt tttggctttt cgcacaataa
     7261 acggtaagag gaattggcac tgaaccaggg tagtccggca gagtaacggt gacatcacga
     7321 gcagaaacat cacaaccgcc agtgggcacc accacatcat tattggcgta aatattccac
     7381 acaaactgaa aatcatcgct gttatagttg ttggtctgtc gcaaaataag cacggcaatt
     7441 aatgaaccag ctttaatcgc caccccgccc gcactgctca caggcgtcaa ataaagcgcc
     7501 accggccacg gcttatccgt tctcgaatta taaacaaccc gcggcgtttc gctggtggtc
     7561 ggaaatggat agctactgcc actatatttt acggtcccgg aaaaattaga taacacgccg
     7621 ccgtaagccg agcctcgttg cagtgtgaca tagtctgtaa tggtttccgg ataatcgtta
     7681 tggcaaaaga tttgcgtcga aagatctacg accaggtttt gccccacatt cacgacaggc
     7741 gcaaggttta cataaacatt agcgctgcca ccgccaatag ggatagcggt accattggcg
     7801 gttttacagg cgaatgacca ggcatttacc gaccagccca tcagcagtac agcaaacagg
     7861 gtaatagctc gtttcattac aattatctct tcgggttcag ctgtaggtat aggtgatgct
     7921 aatcactgcc tgaatggttc cctgagtggc tccgccattt accgtcaatg ctctgacctg
     7981 taacgggaag tgcgctgatt gtgaggaatc atccacctga actgttttgg ttgcgccagt
     8041 attcaatgtg ttgccactgt catcctgtag ctctaactgg atgttttgcg cggtcccctg
     8101 gtttttataa tatccggtgc tgtcggctgc cccgctgaag ctggcagtga ccctcgacgt
     8161 tcccaccgga caattagtca actcaagcgc aacatcatgc caggccgatg ccgccccggc
     8221 agacataaga ctgaaagaat aaagatcgcc gagatctacc gtggcattgg tggtggaaac
     8281 cgtacacggt ttggcgacga ccttaccgtt caccgtgatg gtgacatcgg ctgcctgtat
     8341 cgtcgcactt gcgagcgcca acattgccgc caatacatac ccacgtttgc accatttcat
     8401 gagcatctcc agttactgat attcaagagt gaaggtagcc gtagcattga tatgccccgc
     8461 agtgacaggc acctgtgtcg ccattagccg ggcgtaaaaa ttcagcgtat ttggtttacc
     8521 cggcgtcagg gtcgtccacg aaatcgcaga cgatggagca ttaaggggta tttgattttg
     8581 ctgctcattc agaagctgta ttcccagtcc cgcagccgct gacaccgtat tttcaagtgc
     8641 aagcaggttg gcattgtggc tatctgcaac gccggtaaac ccaactttta cggcagaaac
     8701 ggcattacca cagggtgaca gcaaaatacg aaatggaacg acaggagtcg tcgcgccaat
     8761 gttgttaaat tgcttcgccg cgttttccat cagatcaaca gtaaaattgg ttgattcagc
     8821 ggccacacta cagccgttat ctctgacata gccgcggata gtaatcgtgc tatccgcagc
     8881 caaaacgcga cttaccgcca gccacaaaaa agcgcacaga agataaaaag gtttgtttct
     8941 catcacgccc ccttaacgac attcagctga tagctgggtt aataactgct gctgactctc
     9001 tggtggcagt tgataattgg cgacacagtg agcattttcc tcttctcccc atttcacctg
     9061 aacttttccc gctaaaggca ttccgctgag gtaaacctga ccattatccg caacaatgcc
     9121 gctactctgg ctactctctg atgtcaccat cgccccaaac ggcagcggct tattattgtg
     9181 agttagcgtc atgagcagtt ttatcccaac gcgcgcttta aactctgctc gcacgatcgc
     9241 cccacgagtg ggaacaacgt tagcgaccgc gttatctaaa tcgacgttat cagccaaggt
     9301 attggtatcc agcgccactc tattttcccg atattcagtg gcataaggca gcacggcata
     9361 accacgccag tcggtacgca cccccgtctg gttttcgact tttgcatctt ttgcgccagg
     9421 cgctttaaca agcaccaccg tatcgtttaa cggctgcccc agcgttacgc cattggcatg
     9481 agccaatacc ccaccgctga ctccgtaata gagctgctta atatcatcgc tatggctgta
     9541 accgatattg gcattgccgt aaccaccgcg ataattcagc gtggcgtagc ctgtacttcc
     9601 gctattacca tcgcctcccc cggcatagcc ggtttgcacg ctataactga ggttgttgtc
     9661 ttccagcaac gtaccgtata caccagccag attggtcatc cgaccgttga gatcgtgtga
     9721 catgctgtag ctggcactgg catgtcacca ctgagattta ctgtcagaac gcagccagtg
     9781 gctgaaagga atattgacgt taagcgctaa catctgatcc cgtccttttt gccaggcgtt
     9841 tttcgtcagg ctatagctga gcgtccagtt gatatcttcg aacgcagtat ttaatccagc
     9901 ctggaattgc tcatcgacat tactcgttcc ccaataagtt tgatggctac cactcaaata
     9961 cagtgttgat gtgcgcccga gttgctgagt aacggtgagc tgtaatttcc cgcgtttgtt
    10021 ataagcgagg ttgtaatagt cggtgaattt cggcttaacc tgaataactc catcctgtgt
    10081 ttcgatgttg tagccattca ttcgactgta tgttgtatca gcgaaattaa aatatccgct
    10141 ggtcgaataa cggtaaccca ctaactgaat attcgtgcct gattcattga gcgatttgtt
    10201 atagagaaaa cgcaccgatt gtccgtcatg ctgactgtca tcgggaagtg tggaattagc
    10261 ctgcgtcata tccacagaca gagcgcccag tgcccccatg tttttcccga taccgaaatt
    10321 aaaagcacga taacgatccg ccagttgcgt tccaccatat attgtccagc cagccggaag
    10381 gccgtggagt aatgtactct ggaaaaagcg gggtttttcc tgctgcgcat ttccactacg
    10441 gtattctcct gccgtaatgg aataacgagt atgcccttca cgttgcaaaa gcgggactga
    10501 cgaatagggt acggtaaaaa tctgcgtgct gccgtcagcc tctttgatcg ttacctgcaa
    10561 gtcaccacta ttacctgcgg catagatatc gttgatggta aaaggccccg gtggcaccgt
    10621 actattataa atgccatatc cattttgttt aatagtgacc tgtgcagtac cacgagcaat
    10681 accgtggatc accggggcaa atcctctttg gctatcgggt aacatattgt catctgaggc
    10741 caattgtgcg ccgcgaaagt taataccatc gaaaatatcg ccctgggtat aaccatcacc
    10801 caacgtcagc cgggaacgta aaggaattat gtctcgctca atccaggtaa tgatatgctg
    10861 ccatttattt ttgctacctg atgaactgtc gctactgtta taactccagg tggtattgtc
    10921 gcgtaaacgc cacgcaccaa tatttaaccc actctgtagg tttaaatatg cataatggct
    10981 gttaccccca atccgattct gtacactatt tccgctgaaa ttataattga gcaatccggc
    11041 attaataccg ggatcccata actcaggagg aatataacca cgcgcgcgat tactcataaa
    11101 tgcctgaggg atcgtcaggt tcagtcgctg ctgaccaaca tccagatgcg cagtagcgtc
    11161 ctggaccatt gtggttaatg gcacacaggc atcatccgcc agcagattca taccggcgac
    11221 agaagccgta ttcagcccca tactggcgag ttgcgcgcgt gtcaggcagg gaacaatccc
    11281 ttgttcactg tcgcccgtat taaatgtgac atcacgcgtt gccatataac cattattcaa
    11341 atagatatcg acgcgatacg tccctggcgg taattcttgc ccattttaaa aacgcgataa
    11401 atcggccaca gcctggggat catccgctaa aaagcgcaga ttaaaataga ggtcggcaga
    11461 tgacaaaggt gcctgtgcgg caaaagcaca ggcgacaacg agtcggacaa aaaaaccagc
    11521 caaacgatgc ttacgaatat gcaagcattg tgtgtttcgc tggtaaagtc ttaaattcag
    11581 atatgacatt attcctccgg tttaaaatca cccggcagtc aattcttttt attcaggcga
    11641 aaaattcccc ctgcgttatt ccattacgcc cgtcattttg ggggtaagtg cgccataatc
    11701 atttattgtt cggtaagtaa tattgcttcc tgcatcagaa ggcaatttaa ccgtgctttc
    11761 gcccattgga ggcaccaatg cattttcaag aacccgggtt ccggcattca actctgttac
    11821 cgtcaggtaa tagggtgtcg ggttaatcag cgtcagagaa ttcgcgctac gacgaaatct
    11881 taatttttct gcggcctgat cgggtggcaa cgctaattta gccgggcgat agtacagttt
    11941 aatgcggctg ataattgcga gctgtagcgt attctcagtc aattttgatt tatccattga
    12001 cggaatcgct ttaacgttca tccagaataa actttcccgg tcctgtggca attggttatt
    12061 tgttgcatca agaatacgta aggtattctc tttttttccc ttcatcgcaa acagaggagg
    12121 cgtcacgata aaacgaccat cctttacacc atcggcattt tccacccatg attgaattaa
    12181 ataggtacta ttttcatcat tatttgtcac ggcaagttgc acttgttttt gccctgccgg
    12241 ataaattacg cgagtcgcac ctaaggccac tcctgcttca gcgcgtccgg caaccatcat
    12301 tgccatgaac atcaggatac ctgccagcaa gcagaatgtt atttcctgcg atttccttac
    12361 attgacgttt ttattactca ctgtcctgtt cctgttatca cattatctac tgaacaatta
    12421 ctgataggtt aaagagaacc aggcctgggc attggcgatg ccgccagtaa cccgacgccc
    12481 ggtagcacga tatttggcga tgaaatgtag cgaagtagag cctgagtaaa gccgtttcca
    12541 gtttgctgga ggacgattaa tcggtacgag gtttccttca tcatcaaaca acgctacacc
    12601 aatattggag gctatccctg gcccctctcc cacggagagc acatccggat ttttaccatc
    12661 cgcgacaccg tgaaacgcca cacccacacg ttcactcacc accgtgctac attcccgtaa
    12721 atgaataaca aaaggcaccg gtgcgctatc ttcccctgcc gcatgaaacc ggttactgct
    12781 gatttgcccc atattgaccg tcatttgttt atcaccggct tcaatccggc aagtttccgc
    12841 aataatgacg ccctgaaatt gcatatttcc gccgggcagc gtggtattcc atttatttcc
    12901 ggccagagca aacattggca gcaacgacgc taacagaaat aatctcttcc ttttcatcgt
    12961 tatttttatc gcacaagggt gggcatccct gcccgtaatg acgtccttga acctgggtag
    13021 gttattgata ctgaaccttg aaggttgcat ccgcattagc tgcacccggg gttgcctcgc
    13081 cgattgcata ataacgcgcc tggaacggaa tggtgttggt accgttattc agggttgttt
    13141 gtgcactaaa tgtcgcaccg tccagcgtca gagcattgcc tgttctgtcc aggatctgca
    13201 cgccaacgtt tgttgcacta cctgcagcag aactctgcag agccagtaca tcagtacgcg
    13261 tagcatcaat tgccgtacct aagaaggcaa cagcggcttt tgtggcaaca gtggtatcgc
    13321 aatcattcag ctgaatgtta aaaccaacgg cagagctggt tgctccagcc tgct
//
LOCUS       DADLSA010000104        13239 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.104, whole genome shotgun sequence.
ACCESSION   DADLSA010000104 DADLSA010000000
VERSION     DADLSA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.104"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..328)
                     /locus_tag="JN691_002951"
     CDS             complement(<1..328)
                     /locus_tag="JN691_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244346.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNVRHIFSNKDFVIKFSTSVLQADKKEITIINKNENTTLTQTIATI
                     FEEYLME"
     gene            complement(645..1469)
                     /gene="iclR"
                     /locus_tag="JN691_002952"
     CDS             complement(645..1469)
                     /gene="iclR"
                     /locus_tag="JN691_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAY7244347.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            1669..5352
                     /gene="metH"
                     /locus_tag="JN691_002953"
     CDS             1669..5352
                     /gene="metH"
                     /locus_tag="JN691_002953"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAY7244348.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKWIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYARDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGKDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            5572..7203
                     /locus_tag="JN691_002954"
     CDS             5572..7203
                     /locus_tag="JN691_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAY7244349.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQGLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7294..7971)
                     /gene="pepE"
                     /locus_tag="JN691_002955"
     CDS             complement(7294..7971)
                     /gene="pepE"
                     /locus_tag="JN691_002955"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAY7244350.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFGVTQTWDD
                     YTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGLLAPI
                     TDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTNALPE
                     GHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGEEAVP
                     LEAGHRF"
     gene            complement(8353..9582)
                     /locus_tag="JN691_002956"
     CDS             complement(8353..9582)
                     /locus_tag="JN691_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7244351.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            complement(9634..10422)
                     /locus_tag="JN691_002957"
     CDS             complement(9634..10422)
                     /locus_tag="JN691_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY7244352.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            complement(10433..11230)
                     /locus_tag="JN691_002958"
     CDS             complement(10433..11230)
                     /locus_tag="JN691_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY7244353.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            complement(11296..11790)
                     /locus_tag="JN691_002959"
                     /pseudo
     CDS             complement(11296..11790)
                     /locus_tag="JN691_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(11790..12197)
                     /locus_tag="JN691_002960"
     CDS             complement(11790..12197)
                     /locus_tag="JN691_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HAY7244354.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            complement(12207..13013)
                     /locus_tag="JN691_002961"
     CDS             complement(12207..13013)
                     /locus_tag="JN691_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7244355.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(13083..>13239)
                     /locus_tag="JN691_002962"
     CDS             complement(13083..>13239)
                     /locus_tag="JN691_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY7244356.1"
                     /translation="LSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNSS
                     TAELLLK"
BASE COUNT         3163 a         3420 c         3482 g         3174 t
ORIGIN      
        1 tttccattag gtattcttca aatattgtag caatggtttg agtgagtgtc gtgttttcgt
       61 ttttattaat tatcgtaatt tctttcttat cagcttgtaa gacggacgtg gaaaatttaa
      121 tgacaaaatc cttgttgcta aagatatgac gaacattttg tctacagttc tctgagaagc
      181 tttttaatag aggcgtcgcc agctccttgc cagaaaattt atcctcgagt tctttataaa
      241 acaattcact cagggtttgg tgttcatttg tctgggctgt attattaata tttgcagaga
      301 aagaactacg aggaatacga gtaatcattt aaatatctca ttgtttattg atgtcttgaa
      361 ttttaactat cagaacagtg aaaaaattta atatgattac aactaaaaaa atatcataaa
      421 tcgctcaatc tcataatgca gccgtaaaag tttcggtgga atgagatctt gcgattttgt
      481 taataattaa gtggtttgat gtttaaaaat aatgctgagt taataaaagt gtgtgaagtg
      541 tatgacatta agttattttg gcgtcaatgc gattaacaga cacccttatt ctattgccac
      601 tcaggtatga tgggcagaat attgcctctg cccgccagaa aaagtcagcg cattccaccg
      661 tacgccagcg tcacttcctt cgccgcttta atcaccatcg cgccaaactc ggtcacgcga
      721 tcatcggtaa tacgtgaaat cggtccggaa atagaaattg cggcaaacgg ttcgcggtgc
      781 tcatcgaaaa tacacgctgc aaggcaacgt agccccagcg catgttcctc atcgtcaaat
      841 gaataacccc gtttgcgcgt ttgggcgaga tcttctttta aatgcacagg agacaccaga
      901 gttgcgtggg tataggcatg taaccctttg cggtgcagca gcttcgtcac ctgttcttcg
      961 ctcagttggg ctaaaaaggc tttacccgca ccggaagcgt gcatcggcaa tttaccgccg
     1021 ataggcgcgg acattcgcat caggtgggta cactgtacct ggtcgataat aatcgcttcg
     1081 tgatcgcttt gatcaagcac cgccatattg accgtttcgc cagactcttc cattagattg
     1141 cgcaggatag ggtgaacaat tgctaacaaa ttacggctct ggagaaagct gctgccgacc
     1201 ataaaggcat gtgcgccgat tgcccagtgt cccagttcgc cgacctgacg cacgaaaccc
     1261 tgctgttgca tcgtggttag caggcggtgg gtcgtggaat tgggtaaccc ggcttgttgc
     1321 gccagctctg tgagtgccac gctgccattg gattcggcaa tccactccag taatttcagg
     1381 ccacgcgtta aagactgaac ctgtccagtc gctggtgcgg tggcaacggc gggttttctg
     1441 ccgcgtttcg cgggaatggg tgcgaccatg acagcctcct ttttctgtat cgtggaaatt
     1501 attttcattt ttattgttag ctaatgcaat agttgctgaa ctgatccgat gagttaatgt
     1561 tgaacaaatc tcatgttgcg tggtggtcgc ttttaccaca gatgcgttta tgccagtatg
     1621 gtttgttgaa tttttattaa atctgggttg agcgtgtcgg gagcaagtgt gagcagcaaa
     1681 gtggaacaac tgcgtgcgca gttaaatgaa cgtattctgg tgttggacgg cggtatgggc
     1741 accatgatcc agagctatcg actgaacgaa gccgattttc gtggtgaacg ctttgccgac
     1801 tggccatgcg acctcaaagg caacaacgac ctgctggtac tcagtaaacc ggaagtgatc
     1861 gccgctatcc acaacgccta ctttgaagcg ggcgcggata tcatcgaaac caacaccttc
     1921 aactccacga ccattgcgat ggcggattac cagatggaat ccctgtcggc ggaaatcaac
     1981 tttgcggcgg cgaaactggc gcgcgcctgt gctgacgagt ggaccgcgcg cacgccggag
     2041 aaaccgcgct acgttgccgg tgttctcggc ccaaccaacc gcacggcgtc tatttctccg
     2101 gacgtcaacg atccggcatt ccgcaacatc acttttgacc agctggtggc ggcctatcgc
     2161 gaatccacca aagcgctggt ggaaggtggt gcggatctga tcctgattga aaccgttttc
     2221 gacaccctta acgccaaagc ggcggtcttt gcggtgaaaa cggagtttga agcgctgggc
     2281 gttgagctgc cgattatgat ctccggcacc atcaccgacg cctccgggcg cacgctctcc
     2341 gggcagacca ccgaagcatt ttacaactca ttgcgccacg ccgaagcgct gacctttggc
     2401 ctgaactgtg cgctggggcc cgatgaactg cgccagtacg tgcaggaact atcacggatt
     2461 gcggaatgct acgtcaccgc ccacccgaac gccgggctgc ctaacgcctt tggtgaatac
     2521 gatctcgacg ccgacacgat ggcaaaacag atacgtgaat gggcgcaagc gggctttctc
     2581 aatatcgtcg gtggttgctg cgggaccacg ccgcaacata ttgcagcgat gagtcgtgca
     2641 gtagaaggat tagcgccgcg caaactgccg gaaattcccg tagcctgccg tttgtctggc
     2701 ctggagccgc tgaacattgg cgacgatagc ctgtttgtga acgtgggtga acgcaccaac
     2761 gtcaccggct ctgctaagtt caagcgcctg atcaaagaag agaaatacag cgaggcgctg
     2821 gatgtggcgc gtcagcaggt ggaaaacggc gcgcagatta tcgatatcaa catggatgaa
     2881 gggatgctcg atgccgaagc ggcgatggtg cgttttctca atctgattgc cggtgaaccg
     2941 gatatcgctc gcgtgccgat tatgatcgac tcctcaaaat gggatgtcat tgaaaaaggt
     3001 ctgaagtgga tccagggcaa aggcattgtt aactctatct cgatgaaaga gggcgtcgac
     3061 gcctttatcc atcacgcgaa attgttgcgt cgctacggtg cggcagtggt ggtgatggcc
     3121 tttgacgaac agggacaggc cgatactcgc gcacggaaaa tcgagatttg ccgtcgggcg
     3181 tacaaaatcc tcaccgaaga ggttggcttc ccgccagaag atatcatctt cgacccgaac
     3241 atcttcgcgg tcgccaccgg tattgaagag cacaacaact acgcgcggga ctttatcggc
     3301 gcgtgtgaag acatcaaacg cgaactgccg cacgcgctga tttccggcgg cgtatctaac
     3361 gtttctttct cattccgtgg caaagatccg gtgcgcgaag ccattcacgc agtgttcctc
     3421 tactacgcta ttcgcaatgg catggatatg gggatcgtca acgccgggca actggcgatt
     3481 tacgacgacc tacccgctga actgcgcgac gcggtggaag atgtgattct taatcgtcgt
     3541 gacgatggca ctgagcgttt actggagctt gccgagaaat atcgcggcag taaagcggac
     3601 gacactgcta acgcccagca agcggagtgg cgctcgtggg acgtgaataa acgtctggaa
     3661 tactcgctgg tcaaaggcat aaccgagttt atcgagcagg atactgaaga agcccgccag
     3721 caggctacgc gcccgattga agtgattgaa ggcccgttga tggacggcat gaacgtggtc
     3781 ggcgatctgt ttggcgaagg caaaatgttc ctgccacagg tggtcaaatc ggcgcgcgtc
     3841 atgaaacagg cagtggcgta tctcgaaccg tttattgaag ccagcaaaga gcagggtaaa
     3901 accaacggca agatggtgat cgccaccgtg aaaggcgatg tccacgacat cggtaaaaat
     3961 atcgttggtg tggtgctgca atgtaacaac tacgaaattg tcgatctcgg cgttatggtg
     4021 cctgcggaaa aaattctccg tatcgccaaa gaagtgaatg ctgatttgat tggcctttcg
     4081 gggcttatca cgccgtcgct ggacgagatg gttaacgtgg cgaaagagat ggagcgtcag
     4141 ggcttcacca ttccgttact gattggcggc gcgaccacct caaaagcgca cacggcggtg
     4201 aaaatcgagc agaactacag cggccctacg gtgtatgtgc agaatgcctc gcgcaccgtt
     4261 ggtgtggtag cggcgctgct ttccgatact cagcgtgatg attttgtcgc tcgtacccgc
     4321 aaggagtacg aaaccgtgcg tattcagcac gggcgcaaga aaccacgcac accaccggtc
     4381 acgctggaag cggcgcgcga taacgatttc gcatttgact ggcaggctta cacgccgccg
     4441 gtggcgcacc gtctcggcgt gcaggaagtg gaagccagca tcgaaacgct gcgtaattac
     4501 atcgactgga caccgttctt tatgacctgg tcgctggccg ggaagtatcc gcgcattctg
     4561 gaagatgaag tggtgggcgt tgaggcgcag cggctgttta aagacgccaa cgacatgctg
     4621 gataaattaa gcgccgagaa aacgctgaat ccgcgtggcg tggtgggcct gttcccggca
     4681 aaccgtgtgg gcgatgacat tgaaatctac cgtgacgaaa cgcgcaccca tgtgatcaac
     4741 gtcagccacc atctgcgtca acagaccgaa aaaacgggat tcgctaacta ctgtctcgct
     4801 gacttcgttg cgccgaagct ttctggtaaa gcagattaca tcggcgcatt tgccgtgact
     4861 ggcgggctgg aagaggacgc actggctgat gcctttgaag cgcagcacga tgattacaac
     4921 aaaatcatgg tgaaagcgct tgccgaccgt ttagccgaag cctttgcgga gtatctccat
     4981 gagcgtgtgc gtaaagtcta ctggggctat gcgccgaacg agaacctcag caacgaagaa
     5041 ctgatccgcg aaaactacca gggcatccgt ccggcaccgg gctatccggc ctgcccggaa
     5101 catacggaaa aagccaccat ctgggagctg ctggaagtgg aaaaacatac tggcatgaaa
     5161 ctcacagaat ctttcgctat gtggcctggt gcatcggttt cgggttggta tttcagtcat
     5221 ccggacagca agtactacgc ggtggcgcaa attcagcgcg atcaggttga agactatgcc
     5281 cgccgtaaag gtatgagcgt ctccgatgtt gagcgctggc tggcaccgaa tctggggtat
     5341 gacgcggact gattcacaaa tctgtcactt ttccttacaa caaacagggc gctcaatgag
     5401 tgccctgtct ctttattaat atgaaacact tatactggaa acaggctgga ataatcttag
     5461 ccggaaaaac gaatgtcaaa aggcacagta aagatgccag tcagcgtgtt tccggtcctt
     5521 tggtcattca attgcttcag gaacatataa cgataaggag aacctgaact cgtgttaacg
     5581 ctgcttcacc tgctttctgc cgtcgccctg ctggtctggg ggactcatat tgttcgaacc
     5641 ggcgtaatgc gcgtcttcgg cgcgcgtttg cgtactgtcc ttagccggag cgtcgaaaaa
     5701 aagccgctcg ccttttgcgc ggggatcggc gttaccgcac tggtacagag cagtaatgcc
     5761 accaccatgc tggtgacctc gttcgtcgct cagggtctgg tagccctcgc accggctctg
     5821 gtcattgtgc tgggggcaga tgtcgggacg gcgctaatgg cgcgtattct caccttcgac
     5881 ttatcctggc tgtcaccgtt acttattttt atcggcgtga tttttttcct cggacgcaaa
     5941 cagtcacgcg ccgggcaact gggccgcgtc ggtattggtc ttgggctgat tttgctggcg
     6001 ctggagttga ttgtgcaggc cgtaacgccg atcacccagg caaacggcgt tcaggtgatc
     6061 tttgcctcgc tgaccggcga tattctgctg gatgcgctga ttggcgcgat gttcgccatt
     6121 atcagctact ccagccttgc tgctgtactg ctgaccgcga ctctgaccgc tgcaggcatt
     6181 atctccttcc ccgtggcgct ctgtctggtg attggagcta acctcggttc cggcctgctg
     6241 gcgatgctca acaacagtgc cgccaatgcc gcagcccgcc gtgtcgcgct gggtagtctg
     6301 ctgtttaagc tggtgggtag cctgattatc ctgccgtttg tccatttgct ggcagagaca
     6361 atggggaagc tgtcattgcc aaaagcggaa ctggtgatct atttccacgt cttctacaac
     6421 cttgtacgtt gcctggtcat gctgccattt gttgacccga tggcacggtt ttgcaaaacg
     6481 attattcgcg atgaaccgga actggatacc cagctacggc ctaaacatct ggatgtcagc
     6541 gcgctggaca cgcccacgct tgctctggcg aacgccgcgc gcgaaaccct gcgcattggc
     6601 gacgccatgg aacagatgat ggaagggctg aataaagtga tgcacggcga gccacggcag
     6661 gagaaagagc tgcgtaagct ggcagatgat atcaacgttc tctacaccgc cattaagctg
     6721 tatctggcgc ggatgccaaa agaagagttg gcggaggaag agtcgcgccg ctgggcggag
     6781 atcatcgaaa tgtcgctcaa ccttgaacag gcctccgata tcgtcgagcg catgggcagc
     6841 gaaattgccg ataaatcgct ggcagcacgg cgggcatttt cgcttgatgg gttgaaggaa
     6901 ctggatgcgc tctatgagca attgctcagt aatttaaagc tggcaatgtc ggtgttcttc
     6961 tctggcgatg tcaccagcgc tcgtcgtttg cgtcgcagca aacatcgttt tcgcattctt
     7021 aatcgccgct attcccacgc ccacgtcgat cgcctgcatc agcaaaacgt gcagagcatt
     7081 gaaaccagtt cgctacattt aggcttactg ggagatatgc agcgcctgaa ctcgctgttt
     7141 tgttcggtgg cttacagtgt gctggaacag ccggatgaag atgaaggacg ggacgagtat
     7201 taatattgtg aatcctgtag gcctgataag acgcgtcaag cgtcgcatca ggcactatga
     7261 acaagatgcc ggatcatgca tccggcaatt cacttaaaaa cggtgaccag cttccagcgg
     7321 aaccgcttct tcaccagcct taaacacata agtggtgttc gggccaccca gcgtagcgtg
     7381 acctttactc actgtgatcc agttaccttc cggcagacca ataatcgtca gttctggcgc
     7441 gacgaccagc agttcgcgaa tacgctgctc acgggtttca cctttatggc cttccggcag
     7501 cgcgttggtg aagtgcgggt tgatttgcaa cgggaacaga tttagcgcat cgaaaccttg
     7561 cggatcgaca atcggcatat cgttggtggt acgaatggtt gggcaagcaa ggttagcgcc
     7621 tgcgctccag ccaatataca gcgcgccacg tttaaccacg tcagtaattg gagccagcag
     7681 cccgcgctcg cggcactgtt tcagcaactg gaaagtatta ccgccgccga caatcacaat
     7741 ttccgcattt tcaatcgcgg caacgggatc gacaacgcta tgaataccgg tgacagaaac
     7801 acccagcgga gcgagaaccg cagccgtttt ctctgtgtaa tcatcccagg tctgcgttac
     7861 gccaaacacc gctgagcggc gaccctgcaa ctgttcagca attagcggca gtgcatgttc
     7921 cagccaggct ttacccggca gcgtcgagtt actcaataaa agcagttcca ttacttctcc
     7981 ggttagtaag aaatcacgga aggcatgcta ccactgcgaa tttaaccact tactgacatg
     8041 gctcacgatg ttacgtattg caccgggcgc tgagactgat gacaaacaca gcattgcctg
     8101 atgcgctacg cttacctggc ctaccagata actgcaatat attgaatttt caaaattatg
     8161 tagaccgaat aaggcttcat tccgcatccg gcataaacaa tgtgtacttt gccaccaatt
     8221 tgagcgcccc ttaaggcgct gatatttttt atccttctcg tgcaaattgt gctgcttcag
     8281 caatcagccg cacatccggt cgcgaagttg ctggggcgac cagccagcga catgcagagt
     8341 taagtagcgc gattaatcat gggaaattgc ctctgcatgg gcgagcaggt attgctccgc
     8401 ctcaccggac cagatcccct gatgacgcgc cagaatcgcc gccagtgctt gatcctgaat
     8461 ctgcgtgagt gacgtcagcg gcaggtattt cccggtatac accagctttt tgccgccgcc
     8521 gacggcaggc aattcaagcg tggtttcgcc cgcggcattc agcccaagaa tatgtgttac
     8581 cacttttgcg gcctgcactt ttttctcttc gatcagtttg accgccgcgc gcatgtcgtc
     8641 ggtattgccg cctgacgtgc ccacgtagtg ggtaaatgca taatgcacat cgtagaaatt
     8701 aattggcgcg ctgaaatgtt tatcctgcgg tccggcgaag aaattcaggc aaccatctgt
     8761 cgccagcaag gaagaggcga gagtcaccag tccttcatta ggcacaaaga cgaaaatatc
     8821 atcgaagccg tgaccgccac tcagcgccat taacgtatca aatgctgcat cggtggcatt
     8881 gagataatga atcagtgttt gcggttctga tggatagtgc ttgcgcgcat aactcaattt
     8941 gtcgttgtcg gtatcggtaa tgacgagcag cgacgggtta actggtccat gcagcgcata
     9001 atcaatcgcc aacagtccca ttggtccggt gccgccgagg atcagcgtgc gcccttgcgg
     9061 gcgaatcccc atcgtgtggt tataactacc ttcctgaaga tgatagttgg cgttgaacgc
     9121 gccaatcacg caggaaagcg gttcaaccag tgagccttca aaataggttt cgccgtcgta
     9181 tgccagcagg caatcttgtt ccatgacctc gttgggaata accacatgcg tcgcttcgcc
     9241 gcctacccac gggaaggagt agccggggca atccgggcgg tcggggagtt gcaggttggc
     9301 ctgaatcaca taacgctgac ccggctggaa tttgtgctgc cactttttac ccacggccag
     9361 aatatcgccg caaaattcgt ggccgatgat gattgggttg gtcgccacat cgtcgggtac
     9421 ttttttatgg ttttcaccca gattggcctc tttccaggaa gagaggcaca ggctgtcagt
     9481 gaccaccgtt gcgaggattt catcctcctg catttcagga aggtcaaagg tttccaggcg
     9541 taaatcacgt ttaccataaa gacgcagagc tgttgttttc ataaaccacc tcatgttgag
     9601 cgatggcctc ggcagtcgtg ccggggcgaa gaatcaggac aggaagccca gagcattgcc
     9661 gataatccct aagccaaaca gggcgaaaat cagccatacc gggttaattt ttttgttgag
     9721 cagacgaacc attagtagcg tcagaccgag cgccagcaaa ccagggcaaa gttggtcgag
     9781 aatgttctgc acggtcatgg tgacggtgga gccatcggcg gcatgcgttt gtgaaaccac
     9841 caacggtacg ttgattgacg tccatttggt caccagcacg cccatcacaa acagtccgag
     9901 aattgacgcg ccttcggtga gtttttgcag cacattcccg cccatatcgc tgacgatatt
     9961 cacccctttg cgaaagccga gctgtaggcc ataccacttc atcgccagac gcaccgcgtt
    10021 gaaaataaag aagaacagca gcgggccgag aatgttgccc gaaagtgcca gagatgcgcc
    10081 gagcgcggcg gtaatcgggc gcagcgttcc ccagaccagt ggatcgccaa ctcctgccaa
    10141 tggtcccatc agaccgactt tgatgccgtt gatggtaccg tcatcaattt ccgcgccgat
    10201 gaccgggcca catacggctg gcgtggtatt gaagaacacc aggtgtcgcc tgagcgccgc
    10261 aacctgatcc tcttttaatg ggtaaagtcg cttgatggcg gggatcatgt cgtagcaaaa
    10321 gcccagcccg tgaatacgtt caaagttaaa ggacgcctgt tgcaggttgg agcgcagaaa
    10381 catgctcacc agatcgctgc gtgtaatttt tctctgttcc atgatggctc cgttaatcgt
    10441 caagttggtc aagggcggtg gaggcggtgg cctggagttg aggttcagct ttacgccact
    10501 gtggatttag ctggatgtag accagggcca tgatcacgcc gacgccaccg aacgccagca
    10561 ggctgagatc gaggtagcca cctgcgagga aaccgaggaa aaagaaaggc atcaaatatt
    10621 tcacgcccat catacgtagc accatggcgt aaccgaccac cacgataaaa ccgccagcaa
    10681 tctgtagtcc tcgggtcaca aattcgggaa tggcgtttaa catattgctg accatatcgg
    10741 cgctgacgaa cagtgagaca atcagtgccg gaatggcgac gcgcagcgcc tgcacgccaa
    10801 gcgcggagac atgcagaata tcgagagtgc gaaaccgcgc ctcttctgct gctttatccg
    10861 ccgcgtgctg gaacactacg gtgatggtac gggcaaacac cgtcagcact tggcctgccg
    10921 cagccacagg caacgcgatg gcgattccgg tggcgatgct ttgctgacca acgataacca
    10981 gaatggcgga gattatgctg gcgagtgcag aatccggaga ttgcgccgcg ccgacgttca
    11041 tccagccgag agctatcaat tccagcgtac cgccgagtat tattccggtt tttaaatcac
    11101 cgagaattaa accaatcacc gtacaggcaa ttaacgggcg atgagtctga aattcatcca
    11161 gcacgctgcc cattccggca atacaggaaa aaagaaatat agcaatgatt tgtagggtac
    11221 ttatttccat gatttgtcac cttttaagtg tcgtcatccc tgcctgttaa agcatatgta
    11281 cacaggcgct attatttaat ttgcgattaa ctgttcgttt attttgtcga taatattgat
    11341 tgagggatct gacgctacga cgcgtaaatc cagtatgaca ccaagattat ttaactcatg
    11401 aaatgcgtta atatcatcat catctaatga aaccgctttg gttaactgtt ttttaccggg
    11461 ccgctaggcc atgccgccaa tattcagggt gcctattttt accccctggc gaaccatcgc
    11521 taaggtatcc tgtgggcgag tgaaaagata aaaaacggtt tcgtcctgat attgcgggtt
    11581 gtgataaacc gcgacggctt tttcaatatt gaccacgtta accttcattc ccggtggcgc
    11641 agcctgacgc aataatgtcc ggcgcacttc atcgttataa acttcgtcat tgcagataat
    11701 aatgcgctgg gcgttagcca ctttcgacca gacggtagtg acctggccgt ggatcagacg
    11761 gtcatcaatg cgggcgagcg taatgttcat cagaaatcct cctctgtcgt tgccagttgt
    11821 ttccagacaa cgcaggtttg ctttgcgatt tgcgtcaggt gttcacacag ttcatcaaca
    11881 ttcatgctgt cctggttatc aaccagttcg agcgccagcg gcagagaaag gccgctgatt
    11941 acccgcaaac gaggattgcg catcgcaagc attgctgccg cattccacgg gctgccgcac
    12001 tgtaaatcca cggcaatcag ccactcatct tgattgtgaa tgcttactaa cttttccagc
    12061 tttaccgcaa tgtctccggc gttttcaccg ggcacaaatt caacggcttc aacattggca
    12121 ttgccgtaaa ccatctgaac cgattccagc atggcgcaag ctaatttgcc gtgggcacaa
    12181 aaaatggcgt tgaccataaa gcctccttag ccgcgcgttt tgccgcccgc aatgttagtg
    12241 gttactccgg tgatgtagct ggcgcgttca gacaacagat aacaaacaaa atcagctact
    12301 tctgctaatc ttccggcgcg cccaatagga atggcgtttt tggtatagcc ttcacgcagc
    12361 tgctcgacgg tgatattgcg cgtccacgcc agcgcctctt catattccgg cgtgcgtagc
    12421 ccggtttttt ccagaatccc gggcgcgata ccgaccacac ggataccgtg tttacccagc
    12481 tctttcgacc aggagcgcgt gaagctattg agcgcggctt tggtagcggc gtaacagctt
    12541 tgtccttctg agccttccag cccactttct gaggaaacat tcacaatcac gccatcatgt
    12601 tgtttgacca tctgtcgcgc caccgcctgc gacatcagaa aaacaccttt ctggttgata
    12661 ttgaccattt tttcgaatgc agcttcgttg agttcatact gcccggcagg cgctttctca
    12721 tcgaccagca gacgcgggaa attgaccccg gcgttattga ccagaccgtc gatgcgacca
    12781 aaacgctgga taatttccgc taccgtatga tttacctctt tggtgctaga aatatcggtc
    12841 ggccaaaact gatagccttt atgaccttca tattgaccat cgccaccgtg aatatcgacc
    12901 atctgtacat tcgcgccttg tgctaataat tcctctacaa tcgccagacc aataccggat
    12961 gcgccgccgg tgacaataat gattttatcc tgcaaattta accacgtttg cataacagac
    13021 tcctgatttt agataatagg gacccgccgt aatttttatt acggtaaaca tcgggaagat
    13081 ttttatttca gtaatagttc agctgtgctg ctattcgtta ccagacaatt aatatatttt
    13141 ccacgcagtg caccaataat tccgctgtat ttttcttcac tcatggcaat gccaatggaa
    13201 tatcgcgcct gctttaattt attcatttcg atagagagc
//
LOCUS       DADLSA010000105        13034 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.105, whole genome shotgun sequence.
ACCESSION   DADLSA010000105 DADLSA010000000
VERSION     DADLSA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.105"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            48..827
                     /gene="srlD"
                     /locus_tag="JN691_002963"
     CDS             48..827
                     /gene="srlD"
                     /locus_tag="JN691_002963"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAY7244357.1"
                     /translation="MNQVAVAIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            932..1291
                     /gene="gutM"
                     /locus_tag="JN691_002964"
                     /pseudo
     CDS             932..1291
                     /gene="gutM"
                     /locus_tag="JN691_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            1358..2131
                     /gene="srlR"
                     /locus_tag="JN691_002965"
                     /gene_synonym="gutR"
     CDS             1358..2131
                     /gene="srlR"
                     /locus_tag="JN691_002965"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAY7244358.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            2124..3089
                     /gene="gutQ"
                     /locus_tag="JN691_002966"
     CDS             2124..3089
                     /gene="gutQ"
                     /locus_tag="JN691_002966"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAY7244359.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(3086..4600)
                     /gene="norR"
                     /locus_tag="JN691_002967"
     CDS             complement(3086..4600)
                     /gene="norR"
                     /locus_tag="JN691_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAY7244360.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEMVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            4787..6226
                     /gene="norV"
                     /locus_tag="JN691_002968"
     CDS             4787..6226
                     /gene="norV"
                     /locus_tag="JN691_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAY7244361.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLNLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELTSEAK"
     gene            6223..7356
                     /gene="norW"
                     /locus_tag="JN691_002969"
     CDS             6223..7356
                     /gene="norW"
                     /locus_tag="JN691_002969"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HAY7244362.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(7484..9736)
                     /gene="hypF"
                     /locus_tag="JN691_002970"
     CDS             complement(7484..9736)
                     /gene="hypF"
                     /locus_tag="JN691_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY7244363.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWLAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(9889..10416)
                     /gene="hydN"
                     /locus_tag="JN691_002971"
     CDS             complement(9889..10416)
                     /gene="hydN"
                     /locus_tag="JN691_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAY7244364.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(10565..11578)
                     /locus_tag="JN691_002972"
     CDS             complement(10565..11578)
                     /locus_tag="JN691_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7244365.1"
                     /translation="MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEES
                     SYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKH
                     SAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSV
                     WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLI
                     ANGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGF
                     DDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIA
                     LSR"
     gene            11835..>13034
                     /gene="ascF"
                     /locus_tag="JN691_002973"
     CDS             11835..>13034
                     /gene="ascF"
                     /locus_tag="JN691_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAY7244366.1"
                     /translation="MAKNYAALARSVIAALGGVNNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISCFICG"
BASE COUNT         3104 a         3617 c         3410 g         2903 t
ORIGIN      
        1 tcgcgccggg atcggttttg aagtttgaat ctgttaagga gtaaaaaatg aatcaggttg
       61 ccgttgccat cggtggtggg caaaccttag gcgcgttcct gtgccacggt ctggctgccg
      121 aggggtatcg cgtcgcggtt gtcgatattc agagcgacaa agccgcaaat gtggcacaag
      181 aaattaacgc cgaatatggt gaaggtatgg cgtacggttt tggtgctgac gccactagcg
      241 agcaaagcgt tctggcgctc tctcgtgggg tagatgaaat ctttggtcgc gtggatttgc
      301 tggtctatag cgccggaata gcaaaagcag cctttatcag cgacttccag ctcggcgatt
      361 ttgaccgttc gctacaagtg aatctggtgg gttatttcct gtgtgcccgt gaattttcgc
      421 gtttgatgat ccgcgacggg attcagggtc gcattattca gatcaactcg aaatccggca
      481 aagtgggcag caaacacaac tctggctaca gcgcagcgaa atttggtggc gtcgggctga
      541 ctcaatcact ggcgctggat ctggcggagt acggcattac ggtgcattca ctgatgctcg
      601 gtaacctgct gaaatcgccg atgttccagt cactgttgcc acaatacgcg accaagctgg
      661 gtatcaaacc ggatcaagtc gagcagtatt acatcgacaa agtaccgctc aaacgcggct
      721 gcgattatca agatgtgctg aatatgctgc tgttctacgc cagtcctaag gcgtcgtact
      781 gcaccggaca gtcgatcaat gtcaccggcg gtcaggtgat gttctgatca acagcggaga
      841 tccgttaagg atctccgtga gactatagaa tgcctgatgc gctacgctca tcaggcatac
      901 aggacttccg ccactacatt aaggaaaagt tatggtatcc gcactcatca ccgtcgccgt
      961 tatcgcctag tgtgcgcaac tggccttagg cggctggcaa atttctcgtt ttaaccgtgc
     1021 cttcgacaca ctatgccagc aagggcgggt tggcgtgggc cgttccagcg ggcgctttaa
     1081 accgcgggtc gtggtcgcca tcgcgctgga cgatcagcag tgcatcgtcg acaccttgtt
     1141 tatgaaagga ctgactgtct tcgcccgacc gcaaaaaatt cccgcaatta ccggcatgca
     1201 tgcgggtgat ttacagcccg atgtgatctt tccccatgat ccactatcac agaatgctct
     1261 atcattggcg cttaaactga aacgtggata atttcgttgt gaatgttact tgcttgcgaa
     1321 gttatcattt tgaaacctaa atcaggtaat cacgcccatg aaacctcgtc agcgtcaggc
     1381 cgccattctg gagtatctgc aaaagcaggg taaatgctcg gttgaagaat tggcgcaata
     1441 ctttgacacc acaggcacaa ccattcgcaa agatctggtc attctggaac atgccggaac
     1501 cgtcattcgt acttatggcg gagtggtgtt gaataaagag gaatccgatc cgcctatcga
     1561 tcataaaacg ctcatcaaca cccacaagaa agagctgatt gcagaagctg ccgttagttt
     1621 tatccatgat ggcgattcga tcattcttga tgctggcagt accgttttgc agatggttcc
     1681 cctgctctcg cgctttaata acatcacggt gatgaccaac agcctgcata tcgtcaatgc
     1741 gctatccgaa ctggataacg aacaaactat cctgatgcca ggcggaacat ttcgcaaaaa
     1801 atcggcctca tttcacgggc aactggcaga gaatgccttc gagcatttca ccttcgataa
     1861 attgtttatg ggcaccgacg gcatcgatct caatgcgggc gtaaccacgt tcaacgaggt
     1921 ttataccgtc agtaaggcaa tgtgcaatgc cgcgcgcgaa gtgattttga tggcggactc
     1981 atcaaagttt ggccgtaaaa gccccaacgt agtttgcagt cttgaaagcg tcgataagct
     2041 aattaccgac gcaggtatcg atccggcgtt tcgtcaggcg ctggaagaga aagggatcga
     2101 tgtgatcata accggagaga gcaatgagtg aagcactact gaacgcggga cgtcagacgt
     2161 taatgctgga gttgcaggaa gcaagccgtt taccggaacg tctgggcgat gattttgttc
     2221 gcgccgccaa tatcatcctg cactgcgaag gcaaagtggt ggtttcggga attggcaaat
     2281 cgggccacat tggtaagaaa atcgccgcaa cacttgccag taccggcaca ccggcttttt
     2341 ttgtccatcc ggcagaagca ctgcacggcg atctggggat gattgaaagc cgcgatgtga
     2401 tgctgtttat ctcttactcc ggtggcgcga aggaactgga tctgattatt ccgcgtctgg
     2461 aagataaatc tatcgcgctg ctggcgatga ccggcaaacc gacgtcaccg ctgggcctgg
     2521 cggcgaaagc ggtgctggat atctccgtag aacgcgaagc ctgcccgatg caccttgcgc
     2581 cgacctccag caccgtcaat accctgatga tgggtgacgc gctggcgatg gcggtcatgc
     2641 aggcgcgcgg ttttaatgaa gaagattttg cccgctccca cccagctggg gcactgggcg
     2701 ctcgcttgct gaataaagtg catcatctga tgcgtcgtga tgacgctatc ccacaggtag
     2761 cgttaaccgc cagcgtgatg gatgcgatgc tggaactcag ccgcaccggt ctggggctgg
     2821 tggcggtatg tgacgctcaa caacaggtac aaggcgtctt taccgacggc gatttacgtc
     2881 gctggctggt aggcggcggc gcactcacca cgccagttaa tgaagcgatg acgacaggcg
     2941 gcaccacatt acaggcacaa agtcgcgcca tcgacgccaa agaagttctg atgaaacgca
     3001 aaatcactgc cgcaccagtg gtagatgaaa acggcaaact caccggcgca attaacctgc
     3061 aggatttcta tcaggccggg attatttaat ccttcaatcc cagacgtttc gccagccgat
     3121 gcaggttggc gacgtcagtt tccagcattc gcgcacaggc tgcccagttg tgatgatttt
     3181 gtgccagtgc ctggcgaata gtttcacgct ggaacgcttc tgtcgcttca cgcaggtttt
     3241 gcttaacaac gggcaccgcc gccgcttctg gcggcggcaa cgtcacctca ggaaaagcaa
     3301 aatgttgcgc ctcaagaatc acttcatcgc cgttgcgggt ggctctcgcc agcactaccg
     3361 cccgatgaat agcatgttcc agttcgcgca cgtttcccgg aaaactgtag tgttgcagta
     3421 aatttcgcgc cccggcactt aataccacgc gggagagccc ctgccgcaaa cgacactgct
     3481 cgcagaaata ccccgccagc agaatgacat catcgccccg ctcacgcagc ggcggcaccg
     3541 aaagtggaaa cacgctcagg cgatgaaaca aatcggcgcg gaatcgccct gccagcacct
     3601 cttcgcgtaa gtcgcggtta gtcgccgcca gcacgcgcac atcgacccgc aaactacggt
     3661 catcgccaac gcgctgaata tcgccatact gcaacaccct cagcagcttg gcctgcaatg
     3721 ccaacgacaa ctcgccgatc tcatcgagaa acagagtacc gttatccgcc atttcaaact
     3781 tcccgctgcg gttactgata gcgccagtaa acgctccttt cacatgcccg aacaactcac
     3841 tttccgccac actttccggc agtgcggcac agttgagata gaccagcgga ttcaccgccc
     3901 gtggcgaggc ttcatgaatc gctttcgcca ccagctcctt accggttccc gtctcaccgc
     3961 tgatcagaac gttgagatcg gacgccgcca ccatctcaat ctcttttttc agttgcgtca
     4021 tgccagggga caagccaatc atctgcgtct gtttcaccgc ttcaaacggc gtggcatcgc
     4081 ctggcagcat attctggctt tccagttgtt caatcagcaa cgcattgctt aacgctcccg
     4141 ccgccagcgc ggcaatcagc cgtaactctt cgtcgctgaa aacatcgaac tgatcgggct
     4201 gcatcccgtc gagcgtcagt gcgccgatca ggttttgtcc gacaaacaat ggcagaccaa
     4261 cgcaggcgtg aaccttcaga ctctcctgcc caggaatcaa accgtcatag ggatcgggca
     4321 attcgctgtc tgcgggaaag cgcaccacat ccccggcgcg ggcaatcgct tccagccgtg
     4381 gatgcccttc cagcgcaaag cgtctaccga gtacatcctt tgccagaccg tcgatggcaa
     4441 gcggaataaa ctgccgcgaa tcgtaacgta gcaacgcaga cgcatcgcac tccagcacct
     4501 gacgtagcgt ggtgatcagg cgctgaaaac gatcctgatg accaatccca cgctgcaatt
     4561 cgatggcgat attcgccagc acatcaacgg aaaaactcat ctttgcctca ctgtcaattt
     4621 gactatagat attgtcatat cgaccatttg attgatagtc attttgacta ctcattaatg
     4681 agcataattt tatttataga gtaaaaacaa tcagataaga aactggcacg caatctgcaa
     4741 ttagcaagac atctttttag aacacgctga ataaattgag gttgctatgt ctattgtggt
     4801 gaaaaataac attcattggg tgggtcaacg tgactgggaa gtgcgtgatt ttcacggcac
     4861 ggaatataaa acgctgcgcg gcagcagcta caacagctac ctcatccgcg aagaaaaaaa
     4921 cgtgctgatc gacaccgtcg accataaatt cagccgcgaa tttgtgcaga acctgcgtaa
     4981 tgaaatcgat ctggcggata tcgattacat cgtgattaac catgcagaag aggaccacgc
     5041 cggggcgctg accgaactga tggcgcaaat tcccgatacg ccgatctact gtactgccaa
     5101 cgctatcgac tcgataaatg gtcatcacca tcatccggag tggaatttta atgtggtgaa
     5161 aactggcgac acgctggata tcggcaacgg caaacagctc atttttgtcg aaacaccaat
     5221 gctgcactgg ccggacagca tgatgactta cctgacaggc gacgcggtgc tgttcagtaa
     5281 cgatgctttc ggtcagcact actgcgacga gcatctgttc aacgatgaag tggatcagac
     5341 ggagcttttc gagcagtgcc agcgttacta cgccaatatc ctgacgccgt tcagccgcct
     5401 ggtaacgccg aaaattaccg agatcctggg ctttaactta ccagtcgata tgatagcgac
     5461 ctcccacggc gtggtctggc gcgataaccc gacgcaaatt gtcgagctgt acctgaagtg
     5521 ggccgctgat tatcaggaag acagaatcac cattttctac gacaccatgt cgaataacac
     5581 ccgcatgatg gctgacgcta tcgcccaggg aattgcggaa accgacccac gcgtggcggt
     5641 gaaaattttc aacgtcgccc gaagcgataa aaacgaaatc ctgaccaatg tcttccgctc
     5701 aaaaggcgtg ctggtcggca cttcgacgat gaataacgtg atgatgccga aaatcgccgg
     5761 gctggtggag gagatgaccg gattacgctt ccgtaacaaa cgcgccagtg ctttcggatc
     5821 tcacggctgg agcggcggtg cggtggatcg cctttccacg cgcctgcagg atgcgggttt
     5881 cgaaatgtcg ctcaacctga aagccaaatg gcgaccagac caggacgctc tggagttatg
     5941 ccgtgaacac ggtcgcgaaa tcgcccgtca gtgggcgctc gcgccgctgc cgcagagcac
     6001 ggtgaatacg gtagttaaag aagaaacctc tgccacgacg acggctgacc tcggcccacg
     6061 aatgcagtgc agcgtctgcc agtggattta cgatccggca aaaggcgagc caatgcagga
     6121 cgttgcgcca ggaacgccgt ggagtgaagt cccggataac ttcctctgcc cggaatgctc
     6181 cctcggcaaa gacgtctttg acgaactgac atcggaggca aaatgagcaa cggcattgtg
     6241 atcatcggtt cgggcttcgc cgcccgccaa ctggtgaaaa atattcgcaa acaggacgcc
     6301 actattccat taaccctgat tgccgccgac agcatagatg agtacaacaa acccgacctc
     6361 agccatgtta tcagtcaggg gcaacgtgcc gacgacctta cccgccagac ggcgggtgaa
     6421 tttgccgggc agtttaatct gcgcctgttt ccgcacacct gggtgacgga tatcgatgcc
     6481 gaagcccatg tggtgaaaag ccagaataat cagtggcaat acgacaagtt agtgctggca
     6541 accggtgcca gcgcctttgt cccgcctgtg cctgggcgtg agttaatgct gacgttaaat
     6601 agtcagcaag agtatcgcgc ctgtgaaacg caactgcggg atgcccgacg cgtgttgatt
     6661 gtcggcggtg gtttgattgg tagcgaactg gcgatggatt tttgtcgtgc aggcaaagcg
     6721 gtcacgctaa tcgacaacgc tgccagtatt ctggcgtcat taatgccacc agaagtaagc
     6781 agccgcttgc agcatcggtt gacggagatg ggcgttcatc tgctgttgaa atctcagttg
     6841 caggggctgg aaaaaacgga ttctggcatt ctggcaacgc tggaccacca gcgtagtatc
     6901 gaagtggatg cggtaattgc cgccaccgga ctgcgcccgg aaacagccct ggcacgacgc
     6961 gccgggctga cgattaatcg cggcgtttgc gtcgatagtt atctgcaaac cagtaatgcc
     7021 gatatttacg cgctgggcga ttgcgcggaa attaacggtc aggtattgcc gttcctgcag
     7081 ccgattcaac ttagcgggat ggtgctggca aaaaatcttc tcggcaataa cacgccgctg
     7141 aaactaccgg cgatgctggt gaaaatcaaa acgccggaat taccgctgca tctggcaggc
     7201 gaaacccagc gtcaggattt acgctggcag attaataccg aacgccaggg aatggtggcg
     7261 cgcggcgttg acgatgctga ccagcttcgc gcctttgtgg tcagtgagga tcggatgaaa
     7321 gaggcatttg tattgttgaa aacgttgccg gtgtaggtgg gctactgtgc ctaaaatgtc
     7381 ggatgcgacg ctggcgcgtc ttatccgacc tacggggacg catgtgtagg ccggataagg
     7441 catttacgcc gcatccggca agggtgtcca aatgcaacac gctttatccg ttctggactt
     7501 cacccgctaa ccaacgcgcc gcagcaataa ccccctgccc cagagacaaa ccgccatcac
     7561 ccgccggtaa actctgtgga aagagcaatg tgaaatcagc gagataatgc gccagacgtg
     7621 cacgcagcaa acggttatga ataaccccgc cgctaaatac cagcgtagtg ataccacgca
     7681 tcgtggcctg ctcacgcatc aacgcggcaa aaccctgcgc cagcgcatca tgaaacgccc
     7741 acgcgcgttg attaaccggt gccagccagt tcagccactg ctgccagaaa gtggcgagat
     7801 ccagttgatt gtccacccgc ggcatcgtca ccggatgcgt cactccgtcg catgaggctg
     7861 cgagcgcctc cagagcacaa gccgcttcac cttcataact taacgtggct ggcgcacagc
     7921 ccagtgccgc cgccactgca tcgaacaacc gcccacacga tgatgccagc ggcgcgttaa
     7981 ttccacgctc aatggcccgc gccagtacgc tccagttttg ctgttgcaca cttgccgttt
     8041 cagggtaatt ctgccactcc ggcacaaagc gcaggcactg cgccagcagg tttcgccacg
     8101 gctgcttcgc cgccaaatcg ccacccggaa gcgccactgc aggcaagccg cccaggtgct
     8161 cacattcgcg atagttcacc cgcaggcact cgccgcccca caaagcgcca ttctccccca
     8221 taccgatacc gtcgagcgtc aaagcaatga catcaccgcc atccagcggc cactgatgct
     8281 ctgccagaca cgccgctgca tgggcatgat gatgcagtac cgtttgcgtc ggcagattca
     8341 tttcgctcgc ccactggctg gagacatagc ccggatgcac atcatgcaca atgtattgcg
     8401 gagtgaaatc gtagatgttt tgcatcaggc gtaacgcttc gcgccactgc atctggatgc
     8461 catcgtcact taaatcgccc agatgctgac tcaacaccgc ttgttcaccg cgcaccaggc
     8521 agaaggtatt tttcagatcc gcgccaagac acagcacagg cggaacattt ttaaagcccg
     8581 gaggcaaaac cagcgcatcc ggcacatacc cccgcgaacg gcgcagcatt tcgccgcttt
     8641 cgcgcaccac tgaatcatcc atccgctgca cgatgtcgcg gttatgtagc aagaatccgt
     8701 cggcaatgcc ctgcaaatcc gccagcgcct gttcgttgct gatagctggt ggtttaccgc
     8761 tcaggttgcc ggaggtcatc accagcgggc attgcagttc ctgtaacagc aaatgctgga
     8821 gcgggttcgc aggcaacatc accccgactt cgttaaggcc aggggcgata tcatcacaaa
     8881 gctcaggtac gtatttttta tccaccagca caatcggcgc agcgggcgtg gtaagcaact
     8941 ggcgcgcagc gtctggtaaa ctttctgcca ctggcaacat gactgccagc ggtttcgccg
     9001 ggcgatgttt gcgctcccgc agtgtcgcca ccgcgttact gttacgtgca tcgcaggcaa
     9061 gatgaaatcc gccaatccct ttgatggcga caatgttgcc catttttaac tgtgcgatag
     9121 ctgcctgtaa tgccgcctct tgttctgcat gttcaccatg acttacccat tcaagatgcg
     9181 ggccacactc cgggcaggcc accggctggg cgtggaagcg acgatcgagc gggtcgcggt
     9241 actctttgtc acaggccgga catagcggaa acgccgccat cacggtaaac gggcggtcgt
     9301 aaggcatcgc gcgaataatg gtgaaacgcg ggccgcagtg ggtacagttg ataaacggat
     9361 aacgataacg ccgctcgcct ggggtattca tttcggcaag gcaagcaggg caagtggcgg
     9421 catccgggac aatttgcgta ttcatggtgc cgccggtgct ctggcggatg gtgaactcgg
     9481 tgggcagttg tgaccagata aacggctcac gctcgacgct atcaatacgc gccagtggcg
     9541 gacagtgctg atgcaattga acaagaaacg tttccgggtc ttccagcagc cggacttcta
     9601 cgccatcgcc gtcattacat acatcgccgt gaagatttaa ttgctgtgcc agctgccaga
     9661 caaacggacg aaaaccgacg ccctgcactt tgccacgaat acgcagttgg acaccgcaag
     9721 atgtgttttt tgccattgag ttattcccgc catcatgaat tgcgtaaccc gccctgccgg
     9781 acacgacagc gtcgtatccg gcagtctcag gtcggcgata ccgcccgctc cgtattctac
     9841 gaatatttcc gggaattcct ttgatgccag aacagttctg taagattttt agaacatcag
     9901 cgccgtacgg cggcgttttt ctgcgctcag ttgttcaagt ttattacgat cgacacaaat
     9961 cagcgcatgg gtcgggcaag ccgccataca cgccgggccg tcttcacgat ggttgcacag
    10021 gtcgcattta ttggcttcgg ctttgtcagc ccgtacattc agacccgcgc cgctgttgcg
    10081 aatcacagga cgtaccacca cttccatcgc gccatacggg caagccacca cgcaggtttt
    10141 gcaaccaatg caacgttcct gcatcacatg aacaaaccct ttatcacggc tgatagcacc
    10201 attcgggcag acgttagcgc acggtgcatc ttcacactga cggcaaactg tcgccgtgga
    10261 aatgttcaca cctttaatga catggatacg cggtaaaaat gtttccgggg tcagcgatgc
    10321 acagtcctga ttttcctgat gagaaaccac gcacgccact tcacaggtac ggcaaccaat
    10381 acatttactc gcgtcagcaa tgatgaaacg gttcatcaaa ttctccagca atgacagtta
    10441 atgcgccgat acattcacaa atcatgccag tttttaattt gctgttattt aaggaaatta
    10501 atttctgtaa tgcaggaaaa aagatgtcat cgacactagt gacgatgaca tgtgatgaca
    10561 atgtttatcg cgaaagggca atgagtgagt cgcggcggat cagttttccg ctaaaggttt
    10621 tcggcggtga gaaatccccg ccatcgagca taaaaatcag ccgtccaata atttcctgaa
    10681 tcatctcagt caccggaatt tttacgctgg agagcgccgg aacgatatag ggggcaattg
    10741 cgatatcatc gaatccgata actgacacct gctctggcac cgctacgccg cgctcgtgca
    10801 acgctttgat cgcacctatc gccatatcgt cgttactggc aactaacgcg ctaaatttag
    10861 ccccacgttc gagcaacgtt tctacccctt cggccccgct ggcgggcgtc catttaccgt
    10921 tagcgataag tttttcattg agcgcaatac catgctgcga cagcgcgtct ttatacccgg
    10981 caagacgttc aatgctggtg ggggaatcca tcgagccggt aaggaaagca atctcctgat
    11041 gcccggcgtt tatcaactct gccacggcgt taaagctggt ctgtttatga tcgcaccaga
    11101 cgctatggct gctattattg cgcaggcggc gattaagcac cattatcggc tgactgtgcg
    11161 cgtcaatgat gtcatcaatc tcatccacgc ttaaaaaacg cgggtaaatc ataatggcgt
    11221 cgcagcgcaa atccagcaga tactgaatcg cctggcgctc ttcttcggcg ctgtgtttac
    11281 catctgccaa tagcaactgt cgccctttct cttccgccat tcgcgcggca tgaaacagta
    11341 attcactaaa ataaatgccg tgataaagcg tgttggtcac taccagcccc agcgtctgag
    11401 tactcttcgc cgacagattg cgggccagca aatttgggcg gtaactgctc tcttctaccg
    11461 cctgaaacac gcgatcttta gtctcctggc tgacgtagcc attacctgaa agcacgcggg
    11521 aaacggtcgc ttttgaaacc ccggcgcgct tcgccacttc cagcatcgtc gtcatcattt
    11581 tcatcccttt acacgcaatc aacgcagtgt actgcaccgt ttgccgattg tccttgcgca
    11641 atcggcggga aaaatattca ggtgaccggt ttcacaaata taaaaaatga acaattaact
    11701 ctcttgctta tttagtgaca actattcatg attttgtgaa accggtttct taattccgtt
    11761 tcagcatagg catttttccg tcacgtcgac tgataacaac tacatctacc ctactgataa
    11821 caggataaaa tccgatggcc aaaaattatg cggcgctggc acgctcggtg atagcggcac
    11881 tgggcggcgt taataacatc tcggcagtca cgcactgtat gacgcgtttg cgctttgtta
    11941 tcaaagatga ccagcttatc gacagcccga cgttaaaaac catccccggc gtgctcggcg
    12001 tggtacgtag tgacaaccag tgtcaggtaa ttatcggcaa tacggtttcg caggcctttc
    12061 aggaagtcgt cagcctgctg ccgggagata tgcagcccgc acagcccgtg ggtaaaccca
    12121 aactcacgct gcgtcgcatt ggtgcgggga tcctcgatgc gctgatcggc accatgtcac
    12181 cgctgatccc ggcgattatc ggcggatcga tggtcaaact gctggcgatg atcctcgaga
    12241 tgagcggcgt gctgacaaaa ggatcgccga ccttaaccat tctgaatgtg attggtgacg
    12301 gtgctttctt cttcctgccg ctaatggtcg cggcatctgc cgccatcaaa tttaaaacca
    12361 atatgtcgct ggcgattgcc atcgcgggtg tgctggtaca tccgagtttt attgaattaa
    12421 tggcgaaagc ggcccagggt gaacatgtcg aatttgccct gatcccggtc accgcggtga
    12481 aatacaccta cacggtgatc ccggcgctgg tgatgacctg gtgcctgtca tatatcgaac
    12541 gctgggtgga tcgcattaca ccggcggtga ccaaaaactt cctcaagcca atgctgattg
    12601 tgttgattgc cgcaccgctg gcaatcctgc tgattggccc gattggtatc tggatcggta
    12661 gcgccatttc ggcgctggtt tacaccattc atggttatct gggctggctt tcagtcgcca
    12721 ttatgggcgc gctgtggcct ctgctggtaa tgaccgggat gcaccgcgtc tttacgccaa
    12781 ccatcattca gaccattgcc gaaaccggca aagaaggaat ggtcatgcca tcagagatcg
    12841 gcgctaacct gtcgctgggc ggttcatcac tggcggtgcc gtggaaaacg aaaaacccgg
    12901 aactgcgcca gacggcgctg gctgcggcgg catcagccat tatggcgggg atttccgaac
    12961 cggcgttata cggcgtggcg atccgcctga aacgtccgct tatcgccagt cttatcagct
    13021 gttttatttg cggc
//
LOCUS       DADLSA010000106        13004 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.106, whole genome shotgun sequence.
ACCESSION   DADLSA010000106 DADLSA010000000
VERSION     DADLSA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.106"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(565..1656)
                     /gene="ychF"
                     /locus_tag="JN691_002974"
     CDS             complement(565..1656)
                     /gene="ychF"
                     /locus_tag="JN691_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAY7244367.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1773..2357)
                     /gene="pth"
                     /locus_tag="JN691_002975"
     CDS             complement(1773..2357)
                     /gene="pth"
                     /locus_tag="JN691_002975"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY7244368.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2635..2913
                     /gene="ychH"
                     /locus_tag="JN691_002976"
     CDS             2635..2913
                     /gene="ychH"
                     /locus_tag="JN691_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAY7244369.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2968..4647)
                     /gene="dauA"
                     /locus_tag="JN691_002977"
     CDS             complement(2968..4647)
                     /gene="dauA"
                     /locus_tag="JN691_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAY7244370.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSTLVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4772..5719)
                     /locus_tag="JN691_002978"
     CDS             complement(4772..5719)
                     /locus_tag="JN691_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAY7244371.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5870..6721)
                     /gene="ispE"
                     /locus_tag="JN691_002979"
     CDS             complement(5870..6721)
                     /gene="ispE"
                     /locus_tag="JN691_002979"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAY7244372.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     SLHRAML"
     gene            complement(6721..7344)
                     /gene="lolB"
                     /locus_tag="JN691_002980"
     CDS             complement(6721..7344)
                     /gene="lolB"
                     /locus_tag="JN691_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAY7244373.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7558..8814
                     /gene="hemA"
                     /locus_tag="JN691_002981"
     CDS             7558..8814
                     /gene="hemA"
                     /locus_tag="JN691_002981"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAY7244374.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8856..9938
                     /gene="prfA"
                     /locus_tag="JN691_002982"
     CDS             8856..9938
                     /gene="prfA"
                     /locus_tag="JN691_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAY7244375.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9938..10771
                     /gene="prmC"
                     /locus_tag="JN691_002983"
     CDS             9938..10771
                     /gene="prmC"
                     /locus_tag="JN691_002983"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY7244376.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10768..11160
                     /locus_tag="JN691_002984"
     CDS             10768..11160
                     /locus_tag="JN691_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAY7244377.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRHMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11164..11974
                     /locus_tag="JN691_002985"
                     /pseudo
     CDS             11164..11974
                     /locus_tag="JN691_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            12010..12864
                     /gene="kdsA"
                     /locus_tag="JN691_002986"
     CDS             12010..12864
                     /gene="kdsA"
                     /locus_tag="JN691_002986"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAY7244378.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3257 a         3258 c         3364 g         3125 t
ORIGIN      
        1 agtggttaaa aaatttggtg aagatttttt gtaataatcc taattagtta tgggattttc
       61 gcttgatgta tctgcataac aaaaacaata tgcgccattt ttgcattact aataagaaaa
      121 agcaaaaaac atcgatattc atttttggtc aatggatttt ctggcctatc gttattattg
      181 tgctactggt tatcattttc atcttgccag gatcgcacgt aatcacgtta ttcggggact
      241 ttatctgcgg gatattaccc ttcaggtaag tccttttctc gccagcatat tggagcgaga
      301 tcaatattga tcagctgtga agagggctga tgtgtagtgg aacaaaaact gacgttatat
      361 cactgctaat gagatatcag tcatctgaac acttatgtct ttaattattc tcgtggttca
      421 ctgtaggcaa taagcacaaa agtgtaggat gttacaagaa tgattaggac tcggtgaaat
      481 gaaaaatcca cgcaattgcg tggattttat atacttttgc gctcttcatg agatttagcg
      541 aaacctcatg agacaataaa ttaattagac gttgaaaagg aagttcatca catcgccatc
      601 tttcacgatg taatctttac cttctgcacg cattttgcct gcttctttcg cgccttgttc
      661 acctttgtaa gtgatgaaat cttcaaacga gatggtttgt gcacggataa agcctttttc
      721 aaaatcagta tggattttgc ccgctgcctg cggcgcggtt gctccaaccg gaatggtcca
      781 tgcacgcact tctttcaccc cagcggtgaa gtaagtttgc aggttcagca gcttataacc
      841 ggcacggatc acacggttca ggcccggttc ttccagccca agctcctgca taaactcgtc
      901 acgttcttcg tcgtccagtt cggcaatgtc tgcttcaaca gcagcacaaa ccggaaccac
      961 aacagaacct tctttcgccg cgatttcacg cacctggtca agatatgggt tgttttcaaa
     1021 accgtcttcg ttgacgttgg cgatgtacat tgttggtttc agcgtcagga agctcaggta
     1081 acgaatagcc gctttctctt cagcgcttaa atccagcgcg cgcagcatac ctgcgttttc
     1141 cagctggggc aggcattttt ccaggaccgc cagctcagct ttcgcgtctt tatcgccacc
     1201 tttggctttc ttctgtacgc gatgaatcgc acgttcgcag gtgtcgaggt ctgccagcgc
     1261 cagttcggtg ttgataactt caatatcgtc agccgggtta actttgccgg aaacgtgaat
     1321 gatgttgtca ttttcaaagc agcgaacaac gtgaccgatc gcttcggttt cacggatgtt
     1381 ggtcaggaac tggttaccca gaccttcgcc tttcgatgcg ccttttacca gaccggcgat
     1441 atcgacaaat tccatggtcg tgggaagcgt acgttgcggt tttacgattt cagccagttg
     1501 atccaggcga gggtcaggca ttggtacgac gcctgtgttc ggctcaatgg tgcagaatgg
     1561 aaagttggcc gcttcaatac cggctttggt cagcgcgttg aacagggtag atttcccgac
     1621 gttgggcaaa ccgacgatac cgcatttgaa tcccatgatt taaatcacct taatatctta
     1681 ataatcaacc tgttattgct aacagattgc agaaatggaa ataactttgc ctattataca
     1741 cggcactcgg caaaaatgcc gcagacaacg acttattgcg ctttaaaggc gtgcaatcgg
     1801 ttcgttgctt tggtcaagcc atctgtaaac cacatttcag tacaacgcgc cgcttcgtcg
     1861 atggcttcat caattaactt ctgttcacta acaggcggtt tgcctaacac aaaaccgaca
     1921 actttatttt tatcgcccgg atgaccgatt ccgatgcgta aacggtgaaa gttagggtta
     1981 ttacccaatt tactgatgat gtctttcagt ccattgtgac caccatggcc accgcccaac
     2041 ttaaatttgg cgacgccagg aggcagatcc agttcgtcgt gggccaccag aatttcgtcc
     2101 ggattaatgc ggaaaaaact ggccatcgcc gcaacggctt tgccgctgag attcataaat
     2161 gtagtcggga ctaacaggcg gacatcttcg cctccaaggg tgactcgcga agtataacca
     2221 aagaatttag cctcttcgcg cagcggagcg cgcaaacgct ctgccagtaa gtcaacgaac
     2281 caggcaccag cattatgtcg cgttgcggcg tattcagcac cggggttcgc caggccgaca
     2341 atcaatttaa tcgtcacgtt tttttgtcct gagtgtgtac ataactggcg cgtagtttac
     2401 tggttgcggc cccgcttgac aaaaaactgc gtatcaaatg cagataacgt aataattgcc
     2461 tgagtggact attagaaagt caaggtgttc aggcgtttat ttgtaaagtt ttgttgaaat
     2521 aagggttgta attgtgatta cgcccgcaca taacccacag ggtgttgtct atactttaca
     2581 cataaggaag aggggtattc cctgttacaa cccagaaagt tccggaggtg acatatgaaa
     2641 cgcaaaaacg cttcgatact cggtaacgtg ctcatggggt tgggtctggt ggtaatggtg
     2701 gtcggcgtgg ggtattcaat cctcaaccag ttaccacagt ttaatatgcc ccagtatttc
     2761 gcacatggtg cagtgctaag tattttcgtc ggtgccattc tctggctggc gggtgcccgt
     2821 gttggcgggc atgaacaggt gtgcgaccgt tactggtggg ttcgtcacta tgacaaacgt
     2881 tgccgccgta gcgataatcg ccgtcatagc taacaaataa tgcagtttgc tgaccagtca
     2941 gtttgcgctg actggtcaat ctcgtactta taaatccgcc atcgccgcgc gacgattcgg
     3001 gaagaacgcg aggcgtcccg ggatcggttg aatgcctgcg cgagccatag ttcgcagtgg
     3061 ctggaattcc acgttgcaca cgcgcagttc acatccttcg ggcagacgct tcacaaaacg
     3121 ctggaacgca tcaagaccac cagcatcaag taccggaacg gcatcccact tcagaatcac
     3181 aatccgtttg ccttcaagac gtgactctag gtccgtgaat aagccttcag cagcagcaaa
     3241 aaacagcggg ccaataacgc gcagaaccag gacatcgtct ggaacatcta cgactaccgg
     3301 tgccaggcga gtcatacgtg cgatacgacg cataaacagc agcgatgcca gcacgatccc
     3361 cacgctgatg gcaataacca tatcaaacag cacggtcagt gacatacaca gcagcatgac
     3421 gatgatgtca tctttcggcg catgacgcag caagtcgacc actttgtgcg cttcactcat
     3481 gttccacgcc accatcaaca gcagggctgc catggcggaa agcggcagcc aggagagcag
     3541 tggtgccagt accagcaggg cgagaataac cagagtagag tggatcaccg ccgagatagg
     3601 ggaagttgcc ccggcacgga cgttagcggc agaacgcgcg atggcagcag tagcggtaat
     3661 accaccaaag aatggagcga tgatattccc cagcccctga ccgaccagtt cactattcgc
     3721 cttgtgctta gtcccggtca taccatccag taccacagcg cagagcagag attcgatcgc
     3781 cccgagcatt gccattgaga atgccgcagg cagcagggtg cgaatagaat cccaggttag
     3841 cgtgaattct gaattaggca gatcccacgg cagcaccagt tgcggcagca gttgcggaat
     3901 accgttaccc tgagaaccat cggccaggac gtagtggaat tgcgaaccga tggtagcaac
     3961 atgtccgccg agcaggttaa caatccccat caccgcgcaa ccagccagca aggccggaag
     4021 gtgaccgggt aaacgaatgc ccagacgcgg ccagaaaacc agaatgccaa gcgtcacaat
     4081 gccaatggca gcatcaccca cattaatggt cggcagcgcc ataaataatg cgccgacttt
     4141 ttgtagataa tgttccggga catgggccat ttgcagaccg agaaaatctt taatctgcat
     4201 ggtaccgatg gtgatcccga tacccgaggt gaaacctaag gtgacggaaa ccggaatata
     4261 ctcaatcaga cgaccaaagc gtgccagccc catcagaatc aaaaagattc ccgacaacaa
     4321 ggtcgcaacc agcagtcctg ccagtccaaa ctgttgcgaa acgggataga gaattaccac
     4381 aaatgccgca gtcggaccgg aaacgctaaa gcgtgaccca cccgttagag caatgacaat
     4441 ccccgcaaca gctgcggtat ataaaccgta ctggggtgcc acaccactac caatagccaa
     4501 cgccatcgcc agcgggatag caataatccc gacggttatc ccggcaatca ggtcacgggt
     4561 aaaccgtgcg gcagtatatt tttctttcca gcaagcgtcg atcagagcgc ggaaaggcat
     4621 cacatgtgag gaaaatattt tgttcacaat aatgtttcat ccgtgagcgc atcatctgtc
     4681 aactaaatgg caggtgaagg aggcataggt catacaaatg gatattacag acaaaaaaac
     4741 ccgccgcagc gggtctttga gccgggttcg attagtgttc gaacatggca gagatcgatt
     4801 cttcgttgct gatacgacga atcgcttcgg ccagcatacc tgacagggtc agagtacgca
     4861 cgttcggcag tgatttgatt tcatcgctca gcggaatggt atcgcagaca acgacttcat
     4921 caattacaga gttacgcagg ttgttcgccg cgttgccaga gaagatcggg tgagtcgcgt
     4981 acgcaaatac acgtttagca ccacgttctt tcagcgcttc agcagcttta cacagcgtac
     5041 cgccagtgtc gatcatatca tcgaccagta cgcagtcacg acctgcaacg tcaccgatga
     5101 tatgcatcac ctgggaaacg ttcgcacgcg gacgacgttt gtcgatgatt gccatatcgg
     5161 tatcgttcag cagcttagcg atagcgcggg cacgcacaac gccgccgatg tccggagaaa
     5221 ccacaattgg gttatccaga ttcagctgca gcatgtcttc cagcaggatc gggctaccaa
     5281 atacgttatc aaccggaacg tcgaagaaac cctgaatctg ttcagcgtgc agatccactg
     5341 tcagcacacg gtcaacaccg acgctggaga ggaagtctgc aaccactttc gcagtgattg
     5401 gtacacgagc ggaacggacg cgacggtcct ggcgcgcata gccaaagtag gggataacag
     5461 cggtgatacg acctgcggaa gcacgacgca gggcatcaac cataacgact aattccatca
     5521 ggttgtcgtt agtaggggca caagtggact ggatgatgaa aatatcacca ccgcgtacat
     5581 tttcgttaat ttgtacgctg acttcgccgt cgctaaagcg acctacagcg gcgtcgccga
     5641 gtgaagtgta caggcggttg gcaatacgtt gtgctagtac cggggtggcg ttaccagcaa
     5701 aaagcttcat atcaggcacg agaagaacct caggcatgcg tccattggtg gaaagaatct
     5761 gccgaaaact gtgcgggcca ggcatgatcc tttccaggcg gtgtattaaa gagcgcgatg
     5821 caacgtctgg aacaaggtga cgttgtcacc gaaactcagc ttgcccggct taaagcatgg
     5881 ctctgtgcaa tgaggaaaga ttaacgcctt tcgccacaaa gccattgagc cattccgggg
     5941 cttgctctag cacctggcgg gcttcagact ctgtatcaaa ttcagcaaag acacaggccc
     6001 ctgtcccagt caggcgcgac ggggcgtatt ctaacagcca ggaaagcacc gcatcaacct
     6061 cgcgaaaacg ttttcttgcg ataacctcgc aatcattgct gaattcacat tttagcaacg
     6121 tttctattga cctttttggc gtattgcgcg ggagttcagg atctttaaaa atcaccggag
     6181 tcggaatact tacaccaggg tgcgccacca gataccactt ctctggcgga tccaccggcg
     6241 ttagtatttc accaacgcct tcggcaaacg cggcatgccc ccgaacaaag acaggaacat
     6301 ctgcgcccag cgtcagcccc atttccgcca gctcatccat gcttagcccg cattgccaga
     6361 gatgatttaa tgccaccagg accgtcgcgg cattggatga accaccgccg agaccgccgc
     6421 ccatcggcaa acgcttgtca atgctgatat tcgcaccgct tcccgtcgga agacgcccgc
     6481 tgtctgccgc agttttcatc aacaaccgcg ctgcgcgaac gatcaggtta tcttcatgtt
     6541 ccacgccttc aacgggcgtt aacagacgaa tatccccatc gtcacgaagc tcaatgctga
     6601 tggtgtcgcc gtaatcaaga aactgaaaca gcgtttgcag cgtgtggtaa ccatccgcac
     6661 gctgaccggt aatgtataaa aacagattaa gttttgccgg agagggccac tgtgtccgca
     6721 ttatttcact atccagttat ccatttttaa cttgatgcgt tgaccaccgt cggtgagttc
     6781 catattggct ggcatcgcag gttgcgtttt ggtgtcataa ccaccataaa caaccttcca
     6841 gtttttgcca ttctggctgt aggtaatttc gctcaggcgg tactggtcgt ccagtttgta
     6901 gtcggttgca tcacccggta aacctaaaat ccactggcgc aagctgttga gcggaattgg
     6961 cattccggtc aatttgccaa tcatctcttc ggcgtcatcg gcggtataac gctgaccttt
     7021 attgtcgact aactgcacgt tacccggttg agcattcagc tccagttccg tgctgcccaa
     7081 tgggttagtg agcagcagac ggtagcgatc ctggccggtt tgctgccaga aaaagcgggc
     7141 gtacactttt tgttggtcag aaatataagc gaacgcgccg cgagtctgat actgattaag
     7201 attgcgcacg tcttgctgat gctgacgcca ttgtggcgaa tccgggcttt tgccaggacc
     7261 tttgggcgtg gtaacggaac aggcagtgag cacaagagca gccagcggta gcaggcggat
     7321 aagacgaaaa tcgggcaggg gcatagtgat gacaagtcct tgagatacgt tgcagttata
     7381 acccttaatg ctagcgttac cgtccgctat cgtctatgtt caagttgtct taattgccag
     7441 aatctaacgg ctttcggcaa ttactccaaa agggggcgct ctcttttatt gatcttacgc
     7501 atcctgtatg atgcaagcag actaacccta tcaacgttgg tattatttcc cgcagacatg
     7561 acccttttag cactcggtat caaccataaa acggcacctg tatcgctgcg agaacgtgta
     7621 tcgttttcgc cggataagct cgatcaggcg cttgacagcc tgcttgcgca gccgatggtg
     7681 cagggcggcg tggtgctgtc gacgtgcaac cgcacggaac tttatcttag cgttgaagag
     7741 caggataacc tgcaagaggc gttaatccgc tggctttgcg attatcacaa tcttaatgaa
     7801 gaagatctgc gtaaaagcct ctactggcat caggataacg acgcggttag ccatttaatg
     7861 cgtgttgcca gcggcctgga ttcactggtt cttggggagc cgcagatcct cggtcaggtt
     7921 aaaaaagcgt ttgccgattc gcaaaaaggt catatgaagg ccagcgaact ggaacgcatg
     7981 ttccagaaat ctttctctgt agcgaaacgc gttcgcactg aaacagatat cggtgccagt
     8041 gctgtgtctg tcgcttttgc ggcttgtacg ctggcgcggc agatctttga atcgctctct
     8101 acggtcacag tgttgctggt aggcgcgggc gaaaccatcg agctggtagc gcgtcatctg
     8161 cgcgaacata aagtacagaa gatgattatc gccaaccgca ctcgcgaacg tgcccaaatt
     8221 ctggcagatg aagttggcgc ggaagtgatt gccctgagtg agatcgacga acgtctgcgc
     8281 gaagccgata tcatcatcag ttccaccgcc agcccgttac cgattatcgg gaaaggcatg
     8341 gtggagcgcg cattaaaaag tcgtcgtaac caaccaatgc tgttggtgga tattgccgtt
     8401 ccgcgcgatg ttgagccgga agttggcaaa ctggcgaatg cttatcttta tagtgtggac
     8461 gatctgcaaa gcatcatttc gcacaacctg gcgcagcgta aagccgcagc ggttgaggcg
     8521 gaaactattg tcgctcagga aaccagcgaa tttatggcgt ggctgcgagc acaaagcgcc
     8581 agcgaaacca ttcgcgagta tcgcagccag gcagagcacg ttcgcgatga gttaaccgcc
     8641 aaagcgttag cggcccttga gcagggcggc gacgcgcaag ccattatgca ggatctggca
     8701 tggaaactga ctaaccgctt gatccatgcg ccaacgaaat cacttcaaca ggccgcccgt
     8761 gacggggata acgaacgcct gaatattctg cgcgacagcc tcgggctgga gtagcagtac
     8821 atcattttct ttttttacag ggtgcattta cgcctatgaa gccttctatc gttgccaaac
     8881 tggaagccct gcatgaacgc catgaagaag ttcaggcgtt gctgggtgac gcgcaaacta
     8941 tcgccgacca ggaacgtttt cgcgcattat cacgcgaata tgcgcagtta agtgatgttt
     9001 cgcgctgttt taccgactgg caacaggttc aggaagatat cgaaaccgca cagatgatgc
     9061 tcgacgatcc tgaaatgcgt gagatggcgc aggatgaact gcgcgaagct aaagaaaaaa
     9121 gcgagcaact ggaacagcaa ttacaggttc tattactacc aaaagatcct gatgacgaac
     9181 gtaacgcctt cctcgaagtc cgtgccggaa ccggcggcga cgaagcagcg ctgtttgcag
     9241 gcgatctgtt ccgtatgtac agccgttacg ccgaagcccg ccgctggcgg gtagaaatca
     9301 tgagcgccag cgagggtgaa catggtggtt ataaagagat catcgccaaa attagcggtg
     9361 atggtgtgta tggtcgtctg aaatttgaat ccggcggtca tcgcgtgcaa cgtgttcctg
     9421 ctacggaatc gcagggtcgt attcatactt ctgcttgtac cgttgcggta atgccagaac
     9481 tgcctgacgc agaactgccg gacatcaacc cagcagattt acgcattgat actttccgct
     9541 cgtcaggggc gggtggtcag cacgttaaca ccaccgattc ggcaattcgt attactcact
     9601 tgccgaccgg gattgttgtt gaatgtcagg acgaacgttc acaacataaa aacaaagcga
     9661 aggcgctttc agtgctcggt gctcgcatcc acgctgctga aatggcaaaa cgccaacagg
     9721 ccgaagcgtc tacccgtcgt aacctgctgg ggagtggcga tcgcagcgac cgtaaccgta
     9781 cttacaattt cccgcagggg cgcgttaccg atcaccgcat caacctgacg ctctaccgcc
     9841 tggatgaagt gatggaaggt aagctggata tgctgattga accgattatc caggaacatc
     9901 aggccgacca actggcggcg ttgtccgagc aggaataatg gaatatcaac actggttacg
     9961 tgaagcaata agccaacttc aggcgagcga aagcccgcgg cgtgatgctg aaatcctgct
    10021 ggagcatgtt accggcagag ggcgtacttt tattctcgcc tttggtgaaa cgcagctgac
    10081 tgacgaacaa tgtcagcaac ttgatgcgct actgacacgt cgtcgcgatg gtgaacccat
    10141 tgctcattta accggggtgc gagaattctg gtcgttgccg ttatttgttt cgccagcgac
    10201 cttaattccg cgcccggata cggagtgtct ggtggagcag gcactggcgc ggttgcctga
    10261 acaaccttgc cgtattctcg atctcgggac gggtaccggg gcgattgcgc ttgcgctggc
    10321 tagcgagcgc ccggactgtg aaattatcgc tgtagatcgt atgcctgatg ctgtctccct
    10381 ggcacaacgt aatgcccagc atctggcgat caaaaatatc cacattctgc aaagcgactg
    10441 gtttagcgcg ctagccgggc agcagtttgc gatgattgtc agcaatccgc cgtatattga
    10501 cgagcaggac ccacatcttc aacaaggcga tgtccgcttt gagccgctca ctgcgctggt
    10561 tgcggcagac agtggaatgg cagacatcgt gcatatcatc gaacagtcgc gtaacgcgct
    10621 ggtatccggc ggctttctgc ttctggaaca tggctggcag cagggcgaag cggtgcgaca
    10681 agcatttatc ctcgcggggt atcatgacgt cgaaacctgc cgtgactatg gtgataacga
    10741 gcgcgtaacg ctcggccgct attatcaatg acaagttttt ctacactgct tagtgttcat
    10801 cttattagta tcgcgctttc tgttgggcta ttaaccttac gtttctggct acgttatcag
    10861 aagcatccac aggcatttgc tcgctggacg cgcattgtgc cgccggttgt cgatacggtg
    10921 ttattgttaa gcggcattgc gttgatggct aaagcgcaca tcctgccatt ttccgggcag
    10981 gcacagtggc tgactgaaaa gctgtttgga gttatcattt atatcgtttt gggttttatt
    11041 gcactcgatt atcgtcatat gcacagtcag caggcgcgca ttattgcctt cccgctggcg
    11101 ttggtggtgc tgtacatcat cattaaactc gccaccacaa aagtaccgtt actggggtaa
    11161 gtcatgagat cgttagctga tttcgaattt aataaagcgc cattgtgcga aggcatgatc
    11221 ctggcttgcg aagcaatccg ccgcgatttt ccctcgcaag atgtttacga cgaactggag
    11281 cgtctcgtta gtctggcgaa ggaagaaatc agccagcttc tgcctttaga agagcagctg
    11341 gaaaaactga tcgcgctgtt ttacggcgac tggggattta aagcctcacg cggcgtttat
    11401 cgtctttccg atgcattatg gctggaccag gtgttaaaga atcgacaggg cagtgcggta
    11461 tcattaggtg cggttttatt atgggtcgcg aatcgtctcg atttgccgct gctgccggtg
    11521 attttcccta cgcagctgat attgcgcatt gaatgtccgg atggcgaaat ttggctgatt
    11581 aatcctttta acggtgaatc gttaagcgaa catatgctgg acgtatggtt aaagggaaat
    11641 atcagcccgt cggcggaact gttttatgaa aaccttgatg aagctgataa cattgaggta
    11701 atccgcaaat tgctggatac actcaaagcc atcgttgatg gaagaaaatc agatggagct
    11761 ggcgttacgc accagcgaag ctttattaca attcaaccct gaagatccct atgaaattcg
    11821 cgatcgcggg ttgatttatg cgcaactgga ttgcgaacac gttgcgttga acgatttaag
    11881 ttatttcgtt gaacagtgtc cggaagaccc gatcagcgaa atgatccgtg cgcaaataaa
    11941 taacatcgcg cataaacata ttgtgctgca ttaattaatc gacattttac tcaagattaa
    12001 ggcgatccta tgaaacaaaa agtggttagc attggcgaca tcaacgtagc aaatgacctg
    12061 ccgttcgtac tgtttggcgg tatgaacgtg ctggaatctc gcgatctggc gatgcgcatt
    12121 tgcgagcact acgtaactgt gacccagaaa ctgggtatcc cttacgtgtt caaagcctct
    12181 tttgacaaag ccaaccgttc ctccatccac tcttatcgtg gaccgggcct ggaagaaggg
    12241 atgaaaatct tccaggagtt gaagcagact tttggcgtga aaattatcac cgacgttcac
    12301 gaaccaagtc aggcacagcc cgttgctgat gtcgtggatg tgattcagtt gccggcgttt
    12361 cttgctcgcc agactgacct ggttgaagcc atggcgaaaa ccggtgcggt aattaacgtc
    12421 aagaaaccac agtttgtcag cccgggacag atgggtaata tcgttgataa attcaaagaa
    12481 ggcggcaacg aaaaagtgat tctttgcgat cgtggtgcta acttcggcta tgacaacctg
    12541 gttgtcgata tgctgggctt cagcattatg aagaaagtgt ctggtaactc gccggtgatt
    12601 ttcgacgtga cccacgcact gcaatgccgc gatccgtttg gcgcagcttc cggtggtcgt
    12661 cgtgctcagg tggctgagct ggcacgagcc ggtatggcgg taggtctggc ggggctgttt
    12721 attgaagcgc atccggatcc ggaacatgcg aaatgtgatg gtccatccgc gctgccgctg
    12781 gctaaactgg aaccgttcct caagcagatg aaagcgattg atgatctggt gaaaggtttc
    12841 gaagaactgg ataccagcaa gtaatctttt ttgcttgaaa aataaagtct tagcgttctg
    12901 cgttaagact tttttcatgg gtgccagata caaaaaaggc cgcaggctgt tacccctgcg
    12961 gccggtttcg ggcgcatatt gccatcacgg cagcctgacg cccg
//
LOCUS       DADLSA010000107        12841 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.107, whole genome shotgun sequence.
ACCESSION   DADLSA010000107 DADLSA010000000
VERSION     DADLSA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.107"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(221..2290)
                     /gene="glyS"
                     /locus_tag="JN691_002987"
     CDS             complement(221..2290)
                     /gene="glyS"
                     /locus_tag="JN691_002987"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAY7244379.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2300..3211)
                     /gene="glyQ"
                     /locus_tag="JN691_002988"
     CDS             complement(2300..3211)
                     /gene="glyQ"
                     /locus_tag="JN691_002988"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAY7244380.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3306..3605)
                     /locus_tag="JN691_002989"
     CDS             complement(3306..3605)
                     /locus_tag="JN691_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244381.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3780..4775
                     /locus_tag="JN691_002990"
     CDS             3780..4775
                     /locus_tag="JN691_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7244382.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKSVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4817..5254)
                     /locus_tag="JN691_002991"
     CDS             complement(4817..5254)
                     /locus_tag="JN691_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAY7244383.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5300..5641)
                     /locus_tag="JN691_002992"
     CDS             complement(5300..5641)
                     /locus_tag="JN691_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244384.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIDVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5810..7264)
                     /gene="xylB"
                     /locus_tag="JN691_002993"
     CDS             complement(5810..7264)
                     /gene="xylB"
                     /locus_tag="JN691_002993"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAY7244385.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAMVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7336..8658)
                     /gene="xylA"
                     /locus_tag="JN691_002994"
     CDS             complement(7336..8658)
                     /gene="xylA"
                     /locus_tag="JN691_002994"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HAY7244386.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            9024..10016
                     /gene="xylF"
                     /locus_tag="JN691_002995"
     CDS             9024..10016
                     /gene="xylF"
                     /locus_tag="JN691_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7244387.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            10094..11635
                     /gene="xylG"
                     /locus_tag="JN691_002996"
                     /pseudo
     CDS             10094..11635
                     /gene="xylG"
                     /locus_tag="JN691_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
     gene            11613..12794
                     /gene="xylH"
                     /locus_tag="JN691_002997"
     CDS             11613..12794
                     /gene="xylH"
                     /locus_tag="JN691_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAY7244388.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMCRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
BASE COUNT         3238 a         3247 c         3099 g         3257 t
ORIGIN      
        1 tctgcctttc ggtgaatacc tgagacaaac agtctcaagc acccgtggct attctagctt
       61 aataagtttg tttcttctcc ttgatataat cctaaaaaaa tctcataaaa ttaatatatg
      121 agataatctt tattcagcag aagattatta aaggttgctg tattatttag cgataataaa
      181 aaaagcctgc cagatggcag gctttttaat aacggcgtta ttactgcaac agcgaaatat
      241 ccgcaacgcg caggaacagt tcgcgcagtt tctccagcat ggtcagacgg ttgagacgca
      301 attctttgtc atcaaccatg accatcactt tatcgaagaa agcatcaacc ggctcacgca
      361 gctctgccag ttcgaccagc gcatcctggt aacgaccttc cgcaaagtac ggctccagct
      421 tgtcacgtag caccacaacc tgcatcgcca gtttaatttc ttccggctct ttcagggtag
      481 aggcattcac gcgatcgctc agcacttcgt cagatttcgc caggatgtta gatacacgtt
      541 tgttcgccgc cgccagtgct gcagctgctt ccagggtacg gaagtgcgat accgctttca
      601 tgcgggcatc gaaatcagcc ggacgagtcg gacgacgcgc cagtaccgcc tggatggtgt
      661 caacggtata accttcgtcc tgataccagg cgcggaagcg accgagcata aagtcgataa
      721 catcatcaac tacgttggca ttagtcagct tatcgccata cagacgcacc gcctcttcgg
      781 tcagcgtttg cagatcaagg ttgaggttct tctcaacgat aattcgcagc acgccaagtg
      841 cggcacgacg cagcgcaaac gggtctttgt cgcctttcgg atgctgaccg ataccgaaga
      901 tacccgccag agtatccatc ttatcagcaa tcgccagcgc acaagctacc gggttggacg
      961 gcaggtcatc accggcaaag cgcggctgat actgctcatt cagcgcgacg gctacgtctt
     1021 ccgcttcgcc atcgtgacgc gcgtagtgca tccccataac gccttgggtg tcggtgaact
     1081 cgaagaccat gttggtcatc aggtcacact tagacagcag gcccgcacgg gttgcgtggt
     1141 taacgtcagc gccaatctgt tcggcgatcc agccagccag cgcctggatg cggtcagtct
     1201 tgtcacgcag cgtacccagc tgttgctgga acagcacggt ttgcaggcgc ggcaggttat
     1261 cttccagacg ttttttgcgg tcggtgttga agaagaactc ggcatccgcc agacgtggac
     1321 gaacgacttt ctcgttacca gagataattt gctgcggatc tttcgattcg atgttggcaa
     1381 cgaagataaa gttcggcagc agtttgccgt cgttcgcata caccgggaag tacttctggt
     1441 cacctttcat ggtgtaaacc aaggcttcag ccggcaccgc gaggaatttc tcttcgaatt
     1501 ttgcggtcag aacgaccggc cactccacca gcgaagccac ttcttccagc aggctttcgc
     1561 ttaagtcagc gttaccgcca atcttacgcg ctgcttcttc ggcatcggct ttaatcttcg
     1621 ctttacgttc ttcgtaatcg gcgatgactt tcccgcgctc acgcagaatt tccggatact
     1681 gatcggcgtt gtcgatggtg aattccggtt cgcccataaa gcggtggccg cgaatcacgc
     1741 gatcggactg aatgcccaga atggttgccg gaatgacttt gtcgcccagc agcagggtca
     1801 cggtgtgcac cggacgcacg aagtgcacgt cgcttgcacc ccagcgcatc agtttcggga
     1861 tcggcagttt cgccagcgaa gtcgcaacca tattcggcag cagtgcttcg gtgctttcgc
     1921 ccttcacatg ggcgcgatac agcagccatt cgcctttatc ggtagtcaga cgctcagcct
     1981 ggtcaacggt aataccacaa ccacgcgccc aaccttctgc cgctttgctc ggttttcctt
     2041 cagcgtcgaa cgcctgggca atcgccgggc cgcgtttttc gatttcacga tctggttgtg
     2101 cttccgccag gttagctact ttcagcgcca gacgacgcgg agcagcaaac cattgaacgg
     2161 tgccgtgtgc gaggcctgcg ttatccagct ccgcagtaaa gttcgcagca aaggactctg
     2221 ccaggctgcg cagtgctttt ggtggcagct cttcagtgcc gatttccacc agaaaagttt
     2281 tctcagacat agccgcctct tacttatctt tgttgcacat cgggaagccg agggcttcac
     2341 gggaagcgta atatgcttct gccactgctt tggtcagggt gcgaatgcgc agaatgtagc
     2401 gctgacgctc ggtgacggag atggctttac gcgcatccag caggttgaag ctgtgggcgg
     2461 ctttcagaat acgctcgtag gctggcagcg gcagcggatt ttccagcgcc agcagctgct
     2521 gcgcttcttt ctcgtactgc tcaaagcagg tgaacaggaa gtccacatcc gcgtattcga
     2581 agttgtaagt ggactgctcc acttcgttct gatggaacac gtcgccgtag gtggttttac
     2641 ccagcgggcc gtcgctccag accaggtcgt aaacgctgtc tacgccctga atgtacatgg
     2701 ccagacgttc cagaccgtag gtgatctcgc cggtaaccgg tttacactcc agaccaccaa
     2761 cctgctggaa gtaagtgaac tgcgtcactt ccatgccgtt tagccacact tcccagccca
     2821 gtccccaggc ccccagagtc gggttttccc agttatcttc cacgaaacgg atgtcgtgaa
     2881 tagtcgggtc catgcccagc tctttcagag aaccgaggta cagctcctga atattgtccg
     2941 gcgatggctt aatgaccacc tggaactgat agtagtgctg taaacggttg gggttttcgc
     3001 cgtagcgacc atcggtcggg cgacgagaag gctgaacata agcagccgcc atcggttctg
     3061 gccccagcgc gcgcagacag gtcattgggt gagaggttcc cgcgccgact tccatgtcca
     3121 atggttgaac aatggtgcag ccctggcgag cccagtaatc ctgtaaggtc aggatcaagc
     3181 cctggaaggt cctggtatca aacttttgca tattatttcg tgctggatac gtgtggattt
     3241 aaaggaaggg atcagtatac ccgctggatg gaagatatac agtacgaaac gggaaaaagc
     3301 agggcttaac gcatggaaag atgcaaaaaa tggccatctg cgtcaaaaga acagacaaac
     3361 tgctctttac ggaaggtata accgcgcatt tcatagcttc cctggaactg ttcgaaggcg
     3421 gtgacgtcga ttttttgctc gccggtgtta taacgttttg ccgcctgatc cttgcataac
     3481 gcttccatat tcaacgaacg cagaggatca acctttaccc gttgtgcttt ctgcacgggt
     3541 gacggtgtag aacaacccac tagcaccata agcgccattg ccggaaagaa agcgttcatc
     3601 atcattaaat taccgtctgg tttgcaggtg agtcttatta tttatatcgg tatagtggct
     3661 gatatcttac gaatttcctt aagcctctat aggctgtagt gataaaactc agattattcc
     3721 agtgcgccaa ccaaatgatt tatcggtcag acttttttta accagataca gagacacaga
     3781 tgcagcccaa aatttactgg attgataacc tgcgagggat agcgtgttta atggtggtga
     3841 tgattcacac cactacctgg tatgtgacca atgctcatag tgttagcccc gtcacatggg
     3901 atatcgccaa tgttctgaat tctgcctctc gtgtcagcgt gccgctattt ttcatgattt
     3961 ccggctatct cttttttggc gaacgcagcg cccagccgcg ccatttcttg cgtatcggct
     4021 tatgtctgat tttttatagc gcaatcgcac tgctctacat tgcgctgttt acctccatca
     4081 atatggagtt agcgctgaaa aacctgctgc aaaagtcagt gttttaccac ttgtggtttt
     4141 tcttcgcgat tgcggtgatt tatctggttt caccgctgat tcaggtgaag aacgtcggcg
     4201 gaaaaatgtt gctggtgcta atggtggtga ttggcattat cgctaaccca aacacagtgc
     4261 cgcagaaaat tgacggtttt gaatggctgc caattaactt atatatcaat ggcgatactt
     4321 tttactacat tctgtatggc atgttgggcc gcgctatagg gatgatggac acacagcata
     4381 aagcactgtc gtgggtgagc gccgcgctgt ttgcgactgg ggtttttatt atctcacgcg
     4441 ggacattata tgaattgcag tggcgcggaa attttgccga tacctggtat ctttactgtg
     4501 ggccgatggt ttttatctgc gcaatcgcgc tattgactct ggttaaaaac acgctggata
     4561 cgcgtaccat tcgcggactt ggcttaatct cccgccattc attgggtata tacggattcc
     4621 acgccttgat tatccatgcg ctgcgcaccc ggggaattga gcttaaaaat tggccaatac
     4681 tggatattat ttggatcttt tgcgcgacgt tggcagcgag tttgttactt tctatgctgg
     4741 tacaacgaat cgacagaaac agattagtga gttaagtaaa agcccgttca cattggactg
     4801 accgggctta cgtgaattat tcgctgtatt cttcctgggt aacctgtgtt tcttttggcg
     4861 ggtttattcc ggcatcacga atattcttct gcaccgctac tgcaccaaac aagcttaaga
     4921 agaaagccag tccataaaaa cctttttcac tgagtaataa tgtcgcattc cacagaccta
     4981 ccatcagtag tgcaacagag ataataaaga cagtcaggca ggtcatataa taaatggaag
     5041 tggtcggtat gccttcatac ttgtcccgaa cggtcttttg ataagacgca gcagaaaaca
     5101 gtcctaacac cagtacggca aaataatacc ctttttcatt taactgcatc tctgcattcc
     5161 atagccctaa caggtaggta acgataccac caacgagagc tatccatgac acaatactaa
     5221 aggccggtga ataggttgat attttgttgt ccatcacagt attcctttca ttcctgaatg
     5281 tgtaagagct ttatgttgct cataccgagc gaaacaaacg catttgacca aacaagcaga
     5341 caaaaaaggc gatggggtag atcaccattt cataagcatt gataggtgca tttaaaacat
     5401 cgataaacaa gagaccgata gtgattaacg caactaactg gcagatatgg gtaaaaaaat
     5461 tatccagttg cccgagtttt tctgcgcgaa gatatgcata gttaccaaaa gttgctgtca
     5521 ttaacacgcc aagaaaatag ccttttccac ttagcaatgg gcatgagatc catagcccaa
     5581 ccagataaac cagcgccccg acaacaaata atagttttgc cacagttttt gaggttttca
     5641 taacgatctc catatctacc agcgatacat tacgagtaac caacgaaaga caaaactgaa
     5701 aaatgccatt aacaaatgat tttcagaata aattcatact aaatattaat taattagtga
     5761 gatatataga tgtgaattat cccccacccg gtcaggcagg ggataacgtt tacgccatta
     5821 atggcagaag ttgctgatag aggcgacgga acgtttcacg tcgtggctga taagcggcat
     5881 aacgctgcgc atctggtaga tgcgactgtt ctaacggtag ctgcggcaac aactcaatga
     5941 gcgatttctc tggattcgcc gcaatctgcg ccagccttgc tgcgcccagt gctggcccca
     6001 catccccccc cgtacggtaa tcgagctgct gaccgctgat atccgccagc atctgacgcc
     6061 agtactcact acgcgccccg ccaccaatca acgtaacact ttgcggttta atgccgcagg
     6121 catgcacgac atccatgcca tctgccagcg cataacccac gccttccagc actgctcgcg
     6181 ccagttcatt ggggccatgt tgatgagtca aaccaaagaa aacccccttc gcctggggat
     6241 tattgtgtgg tgtacgctcg ccggaaagat aaggcagaaa ccaaactggc tcggcacttt
     6301 catcagcctg ttgagctgca gcgattaaag ctgggacatt gcacaggccg gttaatttcg
     6361 cgacccaatc cagacacgat gctgcactca gcattacaga cattaaatgc caacgctgcg
     6421 gtagcgcatg gcaaaagctg tgtacagcgc tttctggctt gcttaagaac ccttcgctga
     6481 cagcaaaata gacccccgac gtccccagcg ataacattgc ctgattagca tcaaccattc
     6541 ccacaccaac tgcaccagct gcattgtcgc caccgcctgc gacaactggc accatcgcca
     6601 taccccacgc tttcgcaact tcaggtaaca aagcaccagt aatttcgctg ccttcgtata
     6661 atgcgggcat ctggtcacga gataagtcgc aagcctgcag catgacgtca ctccagtcac
     6721 gctttgcgac atccagccac atggtgccag ctgcgtcaga catatcgctg gcaaactccc
     6781 ccgtcatacg cagacgcaag taatctttcg gtaataatac tttgtcgatt tgacggaata
     6841 tctccggctc atgccgctga acccatagca atttaggcgc agtaaatccg ggcatcatca
     6901 ggttgccggt aatcactcgt gattgcggaa ctctcgcttc cagcaaagtg cactcttgcg
     6961 cacagcgccc gtcgttccac aaaatggcag ggcgcaatac ccgttgttga gcatccagta
     7021 aggttgctcc atgcatctgg ccggcaatac ccaatgcttt aacgtcctgc agagaatgct
     7081 gatcgcccag agctttcatt gcgcgatcag ttgcctgcca ccactgttcc gggtcttgtt
     7141 ccgaccaaag tggatgcggg cgcgaaacgg tcagcttttc cgtttgcgaa gcaaccacct
     7201 caccctgctc gttgagcaaa ataactttta cgcccgaggt gccaagatct atcccgatat
     7261 acatatcgat cgttccttaa aaaaatgccc ggtatcgcta ccgataaccg ggccaacgga
     7321 ctgcacagtt agccgttatt tgtcgaacag ataatgattt accagatttt ccagttgctc
     7381 ctggcgacca ctctgatgca ccggagacaa attatgttcc tgagcatatt tggctaaatc
     7441 tgccagtgac atttggcctt tcaggatttg ctggcccaat tcgctattcc agccggaata
     7501 acgctgcgcg atgcgtttat ccagctcgcc atcttcaatc atgcgcgctg caattttcag
     7561 cgccagtgcc atcgtatcca tcgcgccgat atgaccgtaa aacagatcat atttatcagt
     7621 actttgacga cgtactttgg catcgaagtt cagaccaccg gtggtgaaac cgcctgcttt
     7681 gagaatttca tacatcacca gcgcattctc ttccacactg ttcgggaact ggtcggtgtc
     7741 ccagcccagt tgcgcatcgc cacggttggc gtcgacagaa ccgaacaggc caagcgcaat
     7801 ggcggtggct atttcatgat ggaaagagtg acctgccagc gtcgcgtggt tagcttcaat
     7861 gttcagttta atctcttttt ccagaccaaa ctgtttcagg aagccataga ccgtcgcggc
     7921 atcgtaatca tattgatgtt tagtcggttc ttgcggtttc ggttcgataa gcaacgtacc
     7981 ctggaagccg attttatgtt tatgctcaac caccatctgc ataaagcggc ccagttgttc
     8041 acgctcctga cgcaagtcgg tatttaacag cgtttcgtaa ccttcacgac caccccacag
     8101 gacatagttt tcaccgccca atttatgggt tgcttccatc gctgtaacaa cttgcgttgc
     8161 cgcccagctg aagacttcag gatctgggtt cgtcgccgca cccgcgccat agcgagggtt
     8221 tgtaaagcag ttagcggttc cccacagcag cttcacgccg ctctcttctt gcttggctgc
     8281 caggacatca accatttgcg caaaattatt gatgtactct tttaacgacg cgccctcagg
     8341 ggaaacatcc acatcgtgga agcaataaaa tggcacatgt aacttgtgga aaaactcaaa
     8401 tgcgacatct gctttacgct tcgccaacgc cagtgcctca ccaggctgct gccacggacg
     8461 attaaacgcc cccacaccaa acatgtccgc cccgttccag cagaaggtgt gccagtagca
     8521 ggcggcaaaa cgcaagtgct cttccatacg cttacccaac accagttcgt cgggattgta
     8581 gtgacggaat gctaacgggt ttgaggattt tgagccttca taacgaacgc gatcgagctg
     8641 gtcaaaatag gcttgcatat tgaactccat aatcaggtaa taccgcgggt gatggatgat
     8701 gtcgtaatat tgggcactcc ctttcagttg ctcaattatg ttatttcaca ctgctattga
     8761 gataattcac aagtgtgcgc tcgctcgcaa aataaaatgg gatgatgaaa ctgggtaatt
     8821 cctcgaagat aaaaatgcaa taagtacaat tgcgcaacaa aagtaagatc tcggtcataa
     8881 atcaagaaat aaaccaaaaa tcgtaatcga aagataaaaa tctgtaattg ttttcccctg
     8941 tttagttgct aaaaattggt tacgtttatc gcggtgattg ttacttatta aagctgtcct
     9001 ctaactacag aaggccctac accatgaaaa taaagaacat tctactcacc ctttgtacct
     9061 cacttctgct taccaacgtt gcggcacacg ccaaagaagt caaaataggt atggcgattg
     9121 atgatctccg tcttgaacgc tggcaaaaag atcgagatat ttttgtgaaa aaggcagaat
     9181 ctctcggtgc gaaagtattt gtacagtctg caaatggcaa tgaagaaaca caaatgtcgc
     9241 agattgaaaa catgattaac cggggtgtcg atgttcttgt cattattccg tataacggtc
     9301 aggtattaag taacgttgta aaagaagcca aacaagaagg tattaaagta ttagcttacg
     9361 accgtatgat taacgatgcg gatatcgatt tttatatttc tttcgataac gaaaaagtcg
     9421 gtgaactgca ggcaaaagcc ttggtcgata ttgttccgca aggtaattac ttcctgatgg
     9481 gcggctcgcc ggtagataac aacgccaagc tgttccgcgc cggacaaatg aaagtgttaa
     9541 aaccttacgt tgattctgga aaaattaaag tcgttggtga ccaatgggtt gatggctggt
     9601 taccggaaaa cgcattgaaa attatggaaa acgcgctaac cgccaataat aacaaaattg
     9661 atgctgtagt tgcctcaaac gatgccaccg caggtggggc aattcaggca ttaagcgcgc
     9721 aaggtttatc agggaaagta gcaatttccg gccaggatgc ggatctcgca ggtattaaac
     9781 gtattgctgc cggtacgcaa actatgacgg tgtataaacc tattacattg ctggcaaata
     9841 ctgccgcaga aattgccgtt gaattgggca atggtcagga gccaaaagca gatacctcac
     9901 tgaataatgg cctgaaagat gtcccctccc gcctcctgac accgatcgat gtgaataaaa
     9961 acaacatcaa agatacggta attaaagacg gattccacaa agagagcgag ctgtaagcgt
    10021 tacgccccgg cgcggagcgg gggcgtgatt tctctccatg ccgcgtgaat gaattggctt
    10081 aggtggagtc gttatgcctt atctacttga aatgaagaac attaccaaaa ccttcggcag
    10141 tgtgaaggcg attgataacg tcagcttgcg gttgaatgct ggcgaaatcg tctcactctg
    10201 tggggaaaat gggtctggta aatcaacgct gatgaaagtg ctgtgtggta tttatcccca
    10261 tggctcctac gaaggcgaaa ttatttttgc gggagaagag attcaggcga gtcacatccg
    10321 cgataccgaa cgcaaaggta tcgccatcat tcaccaggaa ttggccctgg tgaaagaatt
    10381 gaccgtgctg gaaaatatct tcctgggtaa cgaaataacc cacaatggca ttatggatta
    10441 tgacctgatg acgctacgct atcagaagct gctcgcacag gtcagtttat ccatttcacc
    10501 tgatacccgc gttggcgatt tagggcttgg gcaacaacaa ctggttgaaa ttgccaaggc
    10561 acttaataaa caggtgcgct tgttaattct cgatgaaccg acagcctcat taactgagca
    10621 ggaaacgtcg gttttactgg atattattcg cgatctacaa cagcacggta tcgcctgtat
    10681 ttatatttcg cacaaactca acgaagtcaa agcgatttcc gatacgattt gcgttattcg
    10741 cgacggacag cacattggta cgcgtgatgc tgccggaatg agtgaagacg atattatcac
    10801 catgatggtc gggcgagagt taaccgcgct ttaccctaat gaaccacata ccaccggaga
    10861 tgaaatatta cgtattgaac atctgacggc atggcatccg gtcaatcgtc atattaaacg
    10921 agttaatgat gtctcgtttt ccctgaaacg tggcgaaata ctgggtattg ccggactcgt
    10981 tggtgccgga cgtaccgaga ccattcagtg cctgtttggc gtgtggcccg gacaatggga
    11041 aggaaaaatt tatattgatg gcaaacaggt agatattcgt aactgtcagc aagccatcgc
    11101 ctaggggatt gcgatggtac ccgaagacag aaagcgcgac ggcatcgttc cggtaatggc
    11161 ggttggtaaa aatattaccc tcgccgcact caataaattt accggtggca ttagccagct
    11221 tgatgacgcg gcagagcaaa aatgtattct ggaatcaatt cagcaactca aagttaaaac
    11281 gtcgtccccc gaccttgcta ttggacgttt gagcggcggc aatcagcaaa aagcgatcct
    11341 cgcccgctgt ctgttactta acccgcgcat cctcattctt gatgaaccta ccaggggtat
    11401 tgatattggc gcgaaatatg agatctacaa attaattaac caactcgtcc agcagggtat
    11461 tgccgttatt gtcatctctt ccgaattacc tgaagtgctc ggccttagcg atcgtgtact
    11521 ggtgatgcat gaagggaaac taaaagccaa cctgataaat cataacctga ctcaggagca
    11581 ggtgatggaa gccgcattga ggagcgaaca tcatgtcgaa aagcaatccg tctgaagtga
    11641 aattggccgt accgacatcc ggtggctttt ctgggctgaa atcactgaat ttgcaggtct
    11701 tcgtgatgat tgcagctatc atcgcaatca tgctgttctt tacctggacc accgatggtg
    11761 cctacttaag cgcccgtaac gtctccaacc tgttacgcca gaccgcgatt accggcatcc
    11821 tcgcggtagg aatggtgttc gtcataattt ctgctgaaat cgacctttcc gtcggctcaa
    11881 tgatggggct attaggtggc gtcgcggcga tttgtgacgt ctggttaggc tggcctttgc
    11941 cacttaccat cattgtgacg ttggttctgg gactgcttct cggtgcctgg aacggatggt
    12001 gggtcgcgta ccgcaaagtc ccttcattta ttgtcaccct cgcgggcatg ttggcatttc
    12061 gcggcatact cattggcatc accaacggca cgactgtatc ccccaccagc gccgcgatgt
    12121 cacaaattgg gcaaagctat ctccccgcca gtaccggctt catcattggc gcgcttggct
    12181 taatggcttt tgttggttgg caatggcgcg gaagaatgtg ccgtcaggct ttgggtttac
    12241 agtctccggc ctctaccgca gtagtcggtc gccaggcttt aaccgctatc atcgtattag
    12301 gcgcaatctg gctgttgaat gattaccgtg gcgttcccac tcctgttctg ctgctgacgt
    12361 tgctgttact cggcggaatg tttatggcaa cgcggacggc atttggacga cgcatttatg
    12421 ccatcggcgg caatctggaa gcagcacgtc tctccgggat taacgttgaa cgcaccaaac
    12481 ttgccgtgtt cgcgattaac ggattaatgg tagccatcgc cggattaatc cttagttctc
    12541 gacttggcgc tggttcacct tctgcgggaa atatcgccga actggacgca attgcagcat
    12601 gcgtgattgg cggcaccagc ctggctggcg gtgtgggaag cgttgccgga gcagtaatgg
    12661 gggcatttat catggcttca ctggataacg gcatgagtat gatggatgta ccgaccttct
    12721 ggcagtatat cgttaaaggt gcgattctgt tgctggcagt atggatggac tccgcaacca
    12781 aacgccgttc ttgattttga taaaaatttt ctcaaagccg gttacgtatt accggttttg
    12841 a
//
LOCUS       DADLSA010000108        12812 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.108, whole genome shotgun sequence.
ACCESSION   DADLSA010000108 DADLSA010000000
VERSION     DADLSA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.108"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(28..387)
                     /gene="frdD"
                     /locus_tag="JN691_002998"
     CDS             complement(28..387)
                     /gene="frdD"
                     /locus_tag="JN691_002998"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY7244389.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(398..793)
                     /gene="frdC"
                     /locus_tag="JN691_002999"
     CDS             complement(398..793)
                     /gene="frdC"
                     /locus_tag="JN691_002999"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY7244390.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(804..1538)
                     /locus_tag="JN691_003000"
     CDS             complement(804..1538)
                     /locus_tag="JN691_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY7244391.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(1531..3339)
                     /gene="frdA"
                     /locus_tag="JN691_003001"
     CDS             complement(1531..3339)
                     /gene="frdA"
                     /locus_tag="JN691_003001"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY7244392.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLD
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3664..4641
                     /gene="epmA"
                     /locus_tag="JN691_003002"
     CDS             3664..4641
                     /gene="epmA"
                     /locus_tag="JN691_003002"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY7244393.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            4860..6362
                     /gene="yjeM"
                     /locus_tag="JN691_003003"
     CDS             4860..6362
                     /gene="yjeM"
                     /locus_tag="JN691_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAY7244394.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMLSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(6513..9824)
                     /gene="mscM"
                     /locus_tag="JN691_003004"
     CDS             complement(6513..9824)
                     /gene="mscM"
                     /locus_tag="JN691_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY7244395.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPML
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYFLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICCALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(9846..10814)
                     /gene="psd"
                     /locus_tag="JN691_003005"
     CDS             complement(9846..10814)
                     /gene="psd"
                     /locus_tag="JN691_003005"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY7244396.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLACNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(10911..11963)
                     /gene="rsgA"
                     /locus_tag="JN691_003006"
     CDS             complement(10911..11963)
                     /gene="rsgA"
                     /locus_tag="JN691_003006"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY7244397.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            12058..12603
                     /gene="orn"
                     /locus_tag="JN691_003007"
     CDS             12058..12603
                     /gene="orn"
                     /locus_tag="JN691_003007"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY7244398.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         2911 a         3334 c         3404 g         3163 t
ORIGIN      
        1 gcgggccgga tttacattgg cgatgcgtta gattgtaacg acaccaatca gcgtgacaac
       61 tgtcaggata gcagccagac cgtagaaaac ccatttgccc gcaggcacgt ggattttcag
      121 atcgtgcatc gcgtggtgca tacggtgtaa accacaccac agcggcagaa cgatcatcag
      181 gaacaggaat acgcgaccaa tgaagctctg cgcgaacgcc agaacgcgct cgtagctcag
      241 cgcatcaccc ggaaacagcc ccagtggcag cagaataccc accagcagga tcatcaccgg
      301 cgcaataatt gcgctccaca taccaccggc cccgaagagg ccccagaata ccggttcgtc
      361 agaacgcttt ggatttggat taatcatctc aggctcctta ccagtacagg gcaacaaaca
      421 ggattacgat ggtggcaacc acagttaccg cccagagact tttgataatt ggctctggtc
      481 ccattttttc gtcttttaca atgatattgg ccgctttcgg tgccagttca aaccaggttt
      541 tggtgtgcag cagagctgcc gtcagagtga tcaggttaat gatcacgata accgggtttt
      601 gtaaaaagtc gacgaatccc gcccaggctt ccgggccatt tttcagggca aacagcccga
      661 aaatcagttc aatgctgaac cacacagccg gaaccgccgt gccttcgcgc agcatgtaaa
      721 agcgataaaa cggcaatttt ttccaccagg tggacgtcat tggccgtaca tacggtttac
      781 gtttagtcgt catgttgcac tccttagcgt ggtttcaggg tcgcgataag aaagtctttc
      841 gaactttcta ctttgccctg ctgaatggcc gcagccggat cgacgtgttt cgggcagact
      901 tcggagcaat agcccacgaa agtacagctc catacaccgt tctggctgtt caactgcgcc
      961 atacgctcct tcttaccgtg gtcgcggcta tcttcgttat aacgatgcgc cagcgtaatg
     1021 gcagccggac cgatgaactc tgggttcagg ccaaactgcg ggcacgcggc gtagcacaga
     1081 ccacagttga tgcaaccgga gaactggtga tactttgcca tctgcgccgg ggtctggatg
     1141 ttagtaccct gatccgcggt gcgggagttg ccgatgatgt acggtttgat cgcttccaga
     1201 ctttcgatga agtgggtcat atcgaccacc agatcgcgtt caatcgggaa gttagctaac
     1261 gcttcaacct tcataccgtc ggtgtaatca cgcaggaagg ttttacatgc cagttttggc
     1321 acgttgttaa ccatcatgcc gcaggaacca caaatcgcca tacggcagga ccagcggtaa
     1381 ctcaggtccg gtgccaggtt gtctttgatg tagcccagcg catccagtaa tgaggtagtt
     1441 gcgtcataag gcacttcata gaatgcgcta tgcggtgcgg tatcgacttc cgggttatag
     1501 cgcaccacct caattttcag gtttttcatc tcagccattc gccttctcct tcttattggc
     1561 tgcttccgcc ttatcggctg catccgcttc gccaccgtaa acgcgtttag ctggcggcag
     1621 cgtagtaatc ttcacgtcgc tgtactccag gcgagtcgtg ccatcagcat cgcggaaggc
     1681 gagggtgtgt ttgagaaagt tgacgtcgtc acgctcggtg caaccttcgt ccagacgctg
     1741 gtgtgcgccg cgggactctt tacgtgccat tgcggagtgc gccatacatt cagcaacgtt
     1801 cagaccgtgg cccagttcaa tggtgtagag caggtcggtg ttgaacacgc tggaggtgtc
     1861 ggtgatgcgc acgcgcttga agcgttcctg cagctctgcc agcttgtcga tggttttctg
     1921 catcagttcc ggcgtacggt agataccgca accttcttcc attgccaggc ccatttcgtc
     1981 gcggatcttc gcccagtttt cgccgccatc ctggttaacc agatctttca gacgttgttc
     2041 aacgccagct gcctgcgctt caattgccgc ttcgttgcca ttaccggcag ttgctgcacg
     2101 ctctgtcgct tgttcaccgg ccagacggcc gaagaccacc agttccgcca gggagttgga
     2161 gcccagacgg tttgcaccgt gcagaccaac agaggaacat tcacccacgg cgaacagacc
     2221 tttaatgcgg gtttcacagt tctgatcggt ttcgataccg cccatggtgt agtgtgcagt
     2281 cggacgtacc ggaatcggtt ctttaaccgg atcgacgcca acgtacgctt tcgccagttc
     2341 gcagatgaac ggcagacgtt catgcagttt tttctcgccg aggtgacgca ggtcgagata
     2401 aaccacatca ccacgcggcg tggagatggt gttgccttta cgccattcgt gccagaaggc
     2461 ctgagaaact ttgtcgcgtg gacccagttc catatatttg tttttcggct cgtccagcgg
     2521 agtttccggg cccatgccgt aatcttgcag ataacggtag ccatttttgt tgaccagaat
     2581 accgccttca ccacggcagc cttcggtcat caggataccg gaacctggca gaccggttgg
     2641 gtgatactga acgaattcca tgtcacgcag cggaacgccg tggcttagcg ccatacccat
     2701 accgtcaccg gtaacgatgc cgccgttggt gttgtaacga taaacgcgac ccgcaccgcc
     2761 ggtagccata acgaccgcgt tagcacggat ctgcaccaac gtgccttcca tcatgttcat
     2821 tgctaccagg ccgcgaacat gaccatcatc aaccagaata tccagcacga aatgttcgtc
     2881 aaaacgctgg atctgcggga attgcagaga ggtctggaac agcgtgtgca gcatatggaa
     2941 gccggtctta tcggcggcga accaggtgcg ctcgattttc atgccgccga agcgacgtac
     3001 gttgacgcta ccatccgggc gacggctcca tgggcatccc cacagttcca gttgggtcat
     3061 ttcggttggg cagtggtgga cgaaataatc cacgacatcc tgctcacaca accagtcgcc
     3121 acccgctact gtatcgtgaa agtgatattc gaagctgtca tgatcctgcg cgacagcggc
     3181 ggagccccct tctgcagcaa cggtatggct acgcatcggg tatacttttg agattagtgc
     3241 gatttttgca tttggatttg cctgcgcggc agcaattgca gcacgtaatc ccgcgccacc
     3301 ggcgcctaca atggcaagat cggcttgaaa ggtttgcacg acattcctcc agattgtttt
     3361 tatcccacag ccccgtactt cagggtaagt gcctgaaagt tacggtctgc gaacgctatt
     3421 ccactgctcc tttataggta caacagtata gtctgatgga taagtctgaa atttgacgag
     3481 atcgattttt ttagtgcgca agagggctaa attatcactg aagatgatta atttaattac
     3541 taaaccatca gatcgtgctt tttttagtca ctgcccaccg ctgtttgatt cctgcgtaaa
     3601 atttgtctcg ccgtcgcgtt gcgagtagac ttcgtgccct tgtcaaaaac tggagattta
     3661 actatgagcg aaacggcatc ctggcagccg agcgcatcca ttcctaactt attaaaacgc
     3721 gcggcgatta tggcggagat ccgtcgtttc tttgccgatc gtggagtgct ggaggtggag
     3781 acgccttgta tgagccaggc gacggtaacc gatattcatt tggtcccgtt tgagacacgt
     3841 ttcgttggcc ccgggcattc gcaggggatg aatctctggt taatgaccag cccggaatac
     3901 catatgaaac gcctgctggt tgctggttgt gggccggtat tccagctgtg ccgcagcttc
     3961 cgtaatgaag agatggggcg ttatcacaac cctgagttca ctatgctgga gtggtatcga
     4021 ccgcactatg atatgtaccg gttgatgaac gaggtggacg atctcttaca acaggtgctg
     4081 gactgcccgg cagcagaaag cctttcttat caacaagctt tcttgcgtta tctggaaatt
     4141 gacccgctct ctgccgacaa aacgcaactg cgggaagtcg cagcgaaact ggatttgagc
     4201 aatgttgctg ataccgaaga agaccgcgac acgttgctac aattgctgtt tacctttggc
     4261 gtagagccaa atattggtaa agaaaaaccg acctttgtgt accactttcc agccagccag
     4321 gcatcactgg cgcaaatcag taccgaagat catcgggtcg ctgaacgctt tgaggtttat
     4381 tataaaggta ttgagctggc gaatggtttc catgaattaa cggatgcccg tgagcagcaa
     4441 caacgctttg aacaagataa ccgtaagcgc gcggcgcgcg gtttgccgca gcaccccatt
     4501 gaccagaatc tgattgaagc cttgaaagtc ggtatgcctg actgttccgg cgtggcatta
     4561 ggtgttgatc gtctggtgat gttggcgctg ggcgcggaga cactggctga agtcatcgcc
     4621 tttagcgttg accgggcata attctgaaaa attactgaat taacagcgaa gaatggcgtg
     4681 ataagcgcca ttctgtagca tatgttttca tttttccgtt aaaggccaca atttaagact
     4741 ggatatttga tatcatccag gtatcaattc tgtattggtt tttacgctgc ctgctcaatg
     4801 ttgcgcagtg cagttcagtg ggcacgtgtt atacacgcgc tgaaatgaag gatggtttca
     4861 tgcctcacac gataaaaaag atgagtctga taggactcat attgatgatc tttacttccg
     4921 tatttggatt tgccaatagc ccatcggctt attacttaat gggttatagt gcgattccct
     4981 tttatatatt ttctgcattg ttattcttta ttccattcgc cttaatgatg gctgaaatgg
     5041 gagctgctta tcgcaaagaa gaaggtggta tctattcttg gatgaataat agtgtcggac
     5101 cacgttttgc cttcattggt acgtttatgt ggttttcctc ttatatcatc tggatggtga
     5161 gtacctccgc gaaagtttgg gtaccgttct caacattcct ctatggtagt gacatgaccc
     5221 agcactggcg tattgccgga ctggagccta cgcaggtggt tggtctgctg gcagtagcat
     5281 ggatgattct ggtcaccgtc gttgcttcaa aggggattaa taaaattgcc cgcattactg
     5341 cggtgggcgg tattgcagta atgtgtctga atttagtatt gctgttagta agcattacta
     5401 ttttgttatt aaatggtggg catttcgcgc aggatattaa tttccttgca tcaccgaacc
     5461 cgggttatca gtccggtctg gcaatgctat cgtttgtggt atttgctatt tttgcctatg
     5521 gcggaattga agcggttggt ggtctggtcg ataaaacgga aaatccagaa aagaactttg
     5581 ccaaaggtat tgtttttgcc gctattgtta tttcaatcgg ttattcgctg gcaatatttt
     5641 tatggggcgt cagcacaaac tggcagcagg tattaagtaa tggttccgtt aaccttggca
     5701 atattaccta tgtgctgatg aagagcctcg gggtgacgct gggtaacgca ctgcatttgt
     5761 cacctgaagc gtcattgtcg ctgggcgtat ggtttgcgcg tattaccgga ctttcgatgt
     5821 tcctcgctta taccggtgcg ttctttacgc tttgctattc accgctgaaa gccatcatcc
     5881 aggggacgcc gaaagcgttg tggccggaac cgatgacgcg cctgaatgcg atggggatgc
     5941 tgtctatcgc catgtggatg cagtgcgggt tggttactgt cttcattctg ctggtttcgt
     6001 ttggtggcgg taccgcatcg gcgttcttta acaagctgac gctgatggcg aacgtgtcta
     6061 tgacgcttcc ttatctgttc ctcgcgctgg ctttcccgtt ttttaaagca cgtcaggatc
     6121 tcgacagacc atttgtgatt ttcaaaacgc gtatgtcggc aatgattgcg acggtggttg
     6181 tcgttctggt ggtgacattt gcgaacgtct tcaccattat tcagcctgtg gttgaagccg
     6241 gagactggga cagcacattg tggatgattg gcggccctgt cttcttctcg ctgttagcga
     6301 tggcgattta ccagaactat tgcagtcgca tggcgaataa acctgagtta gctctcgact
     6361 gatcatatga aatcctgcaa ctcatcgtct ctccggcgat gagttgtcag gaagtaatgc
     6421 gtatggatga tactctccag ggccggtgat aactgaagat gatgttaagc tgctaaatga
     6481 gccacgttga catcatttgg cagcttcatg cattacaaac ttcccgcctg acgacctttg
     6541 cccgcagacg tcagcgttct ccccgtttgt ttaccgttga ggctttccag acgcatctgg
     6601 aagggcggga atggcatatc gataccgtgg gcgtggaagc ctgccagaat cagctggtgg
     6661 atctcatggc gtagcggcat acggtgaccc atctcagcgg cgtaaatacg cagctcgaaa
     6721 atctgaatcc cctgttgcag atccaccagg aagacttccg gtgccgggtt gtcgatcacc
     6781 agcgagcagc gacgcgctgc ggtgagcagg atttccgtca cttcttcact gttggcatcg
     6841 gcaggggccg gaatcgtcaa cactacgcgc gtgaccgagt cagagagcga ccagttgata
     6901 aactgctcgg taataaacgc cttgttcggc acgattatct ctttacggtc ccagtcgctg
     6961 atggtggtgg cgcgggtgtt aatttttgtc acgctaccag taagatcgcg gatcgttacc
     7021 gtatcgccaa tgcgaatcgg tttttcgaac aggatgatca ggccagagat aaagttggcg
     7081 aaaatttcct gcaaaccaaa accgagacca acaccgagcg cggcaaccag ccactgcaat
     7141 ttcgaccact caataccaat cattgagaag ccgaccagcc cgccaatcag catcagcaga
     7201 tattttgtga tggtggtgat ggcgtaaccc gtacccggcg ttaaatccag gtgctgcaaa
     7261 atcgccagtt ccagcagcgc gggcaggttg cgcaccagct gcgtggtgat gataaatacc
     7321 agaatggcaa tcagcaccgc accgagggta attggctcca gactttctac gccctgtacc
     7381 gtggaggtga catcccacag cgaaatattt tcgaggaagc cgaaagcgga atggatttct
     7441 gaccacagca caatgaccga aagcagggcg atcaacatca aaattgagcg caccagccgc
     7501 aaggattgcg cactgatggc atcgagatcg acttcgcttt catcgacttc aattgctcct
     7561 tccgggctac tgtgatgatg cgcttcctct tcgccacgcg cacgttgcgc taacatctct
     7621 gcccggcgat gcttcgcccg gtcaaacgcc agcctgcgac gctggatcag catccagcgg
     7681 cggataacgt gataaaccac cagtagcagg aaccagatgg caaccgaggt ttcaagcctc
     7741 gctaacagtg cctgcgccgt tgccagataa cccaccgccg acgccagaat ggcaaccagt
     7801 ggtgcgccaa tcatcatgtt ccacagcata tggttggtaa tgttgtcgcc gctgccttct
     7861 ttgttgagat acaacggaat accggctttt ttcaggctta gggtgaccac cgccagcgca
     7921 cagcaaatga ggataaagca aagccgtccc agcgaaccgg aaaattcgcg gtcgtcgagg
     7981 ttatcgaaca tcatcagcgc cataatcagc ccgatgctca tcaggaagta gcgcatccca
     8041 cgggaaacac gttcgcgcgg ccagccaaaa tgagcgataa acaagccgtt cgggcgggca
     8101 aaggtggcgc aaatcatcac tacccacagc atcggcacgg tggccgttac accatcgcca
     8161 atcgcgaccg ccagcggata aggccacgcc tcgcgcaagc cgtaacccag cgtcatccac
     8221 agcaccggta acggtgacgc gacgagaatc gaccagaaaa gagtgcgcaa cgtcagccag
     8281 aagtgatcct gagtcacttt gccgactttc gccgccgaac gttcaagaaa acgggtgaaa
     8341 tagcggcgcg agtaaatact gcaaccgacc agaatcaacg cgccaaacag cggcaaaatt
     8401 gtctctttgc tggtcagcat catcacactg gctttgccca actgactgaa ggtgtccagc
     8461 gaaatgagac gacgcagatc ctgggcgatt tccagcggcc aggcgatggt catcgggcgc
     8521 acgtcagagg tccagaacag atagcggtgc gttgcttcgt tcacctcttt cagcgcatcc
     8581 tccagttgcc cgttggagac tttcagcttg gtcagttcca atagtagcgt gtcgccaccc
     8641 tgcaacaatg agttcagcaa ctcacgctga gtgcgcagtt gtgcttccag aatacggttt
     8701 tgctcggcgg tcagcggctg accgtcggcc tgatggattt gccgtagcag cggctgttta
     8761 ttaagtaaat cctcataacg taaccgttgc acacgcaact gcgccatttc ggtatcaagc
     8821 tgttgtggtt ttggcatttc tggtagccgt gccacctgcg cccgcagcgc ttcgccgagc
     8881 aggttggacg atcccagcca ttgcgactgt tcacgcagcg tattcaacgc ctgccggacc
     8941 tgtaacgtct ggctggcagc ctgacgctgt tgcgaggcaa cgagatccat ccgctgcgcc
     9001 tgttgattca gcgctgccga tagttcgcgg ttaattttga attgcgcgac gatatctttc
     9061 ggcaaatcgg cactgttttc tgccagtaat tcggtacttt ccagcgcccg ctccgcctca
     9121 agctgacgtt ggctattcaa ctgattacgt aaggcctgca aatacgcatc cagttgctgg
     9181 ctctcttttt ccgccagctc tgagcgtaag cgcgctaatt cctggcggtt attggcagac
     9241 agctgcgcca gctccagttc atcaacgagc gccttaagac gtgcagagtc agactgcaac
     9301 gcgaaatttt gtgcctgatt gagcggagta ttgccggtaa gcgttcccag gcggcgctcg
     9361 atctcattta actgacggcg ggcgtcggtt tgctgttgcg gcagttgatt cagcgaatcg
     9421 gcaatctcgc gggcgcgctc ctgctcttgc tgggcctgac ggcttttatc cagcaactgg
     9481 ctgctgacct ggagaatttc ctgattcagc gcgtcggtag acattcccgg cgacacgctg
     9541 cgcggctcgt cacgcatgtt gtttaattgt gcgcgcagag tagcggagag tttcggataa
     9601 ttatcgataa cttgctgata ttgtttgatg cgctcaaggg aaccttttcg ttcctcaagc
     9661 gcatttaagg cagactggag cgcctctacg acttccggct gtgcgggttt cgccgctttt
     9721 gcctgctcca gttcctgagt gatttgtttg ctatcggggg ccgtcgcggc gtacgccccc
     9781 caactgaggc accaggccat cagaaaagtg ataatcaggc gcacgtcagc gtttcctttg
     9841 atggattaga cctggtcttt tttatcgtca accaatgggc tggcgtcgtg ttctgcttcg
     9901 atctcttcag caggaagtgg ggcgggttca gcgtctggcg taacaaaggt ttcggtagat
     9961 actgccagcg gctggccaat tttcgtgaca gacaggcttt ccagttgctc aaccagattc
    10021 actttacccg gtgcaaacag gttgataacg gtggaaccga gtttaaagcg acccatttcc
    10081 tggcctttca gcagtgccac agaaccgtcg ttttccccgg caggccaggt ccagcgcttg
    10141 atgatacctt cgcgcggcgg cgtaatggtg cccgcccaga ccgtctcaat gctgccaaca
    10201 atcgtcgctc cgaccagaat ctgcgccatt gggccaaatt cggtatcgaa aaggcaaatc
    10261 acgcgttcgt tacgggcaaa cagattcggc acgttctgag cggtgagatg gttaacggaa
    10321 aagagatcgc ccggcacgta gatcatctca cgcagaatac cgttgcacgg catgtgtacg
    10381 cgatggtagt cacgcgggga gaggtaagtg gtcacaaacg taccgttgcg gaacaggtcc
    10441 gccatcagat agttgcatgc cagcagggct tcgaggctgt agttgtggcc tttggcttgc
    10501 aggattttat cttcttcgat tttacccaac tggctgataa cgccatcggc aggcatgacc
    10561 agtacattcg gatcggtatc gattgggcgt acttcgtcac gcagcggacg gacaaagaat
    10621 tcgttaaagg tgcggtagct ggcggtgtcc ggcttttgcg cctctttcat gtcgaccttg
    10681 tagtatttaa cgaacagatc gataaccagt tttgtcagcc atcctgcccg cttgcttgcg
    10741 ccccaacccg ccaggcgagt aagccatagt ttcggcagaa tgtactgtag cgaaagttta
    10801 aatgaattta acaaggtagc ctccaggcca ttgttttgtc gttcctgatc cggccaacat
    10861 gccggatcct gaaaaaaagg ggacgattct aacgacggtt agcttaattg tcagtcatcc
    10921 gtatcagaaa agtttttacg cgtttttacc tgcgccatgc tttccagaat acggtgatag
    10981 ttttcgaaac gggtttccgc gattttccct tcctcaaccg cttcccggat agcgcagccc
    11041 ggatcggtat cgtgtttgca atcgcgatat ttacacagac ctaaatagtc atggaattcg
    11101 acaaagccct gagtgatttg ttccggctcc aggtgccaga ggccgaactc acgcacacca
    11161 ggggaatcaa tcacatcacc gccgtgcggg aagtgataca gccgagcggc ggtcgtggtg
    11221 tgctgaccga gccccgagtt gtcagagaca tcgttggtca ggatctcttt ttgcagcccc
    11281 agcaacgcgt tcagcaggct ggatttaccg acgccagact gcccggcaaa aatgctgatg
    11341 cgcccggtca acgcctcttc cagcggtttc agcccatcct gagtatggct ggaaaccatc
    11401 aatacgcgat aaccgatgtt gcggtagata tccatctgct cgttgacgaa cgccatgcct
    11461 tcgtcgtcca gcaggtctat cttgttgagc acgataatcg gctcaatctg caaggtttcg
    11521 caggccacca ggtaacggtc gataatattg agcgacagct ccggcaaaat ggcggagaca
    11581 atgacaatct ggtcgatgtt ggcggctatg ggtttcacgc cgtcgtagaa atccggacgc
    11641 gttaacaccg aggtgcgctc atgcaccgct tccacgatcc ctttgacatt tacgccttcc
    11701 gccgccggtt tacccggacg ccagactacg cggtcgccgg ttaccagcga acggatggta
    11761 cggcgaatat tgcagcggtg aacgtcgcca tcggcggatt ccacatcagc gtgcatacca
    11821 aagcggctga tgacgatacc ttcatcaggc tcgccaaaca gattgtcgtc gtagtcgggc
    11881 ttctccttag acgttttaag acgacgctgg tgattggcgt tcacgcggcg ctgctggcct
    11941 ttggagagtt tatttttact caatcgtact gactcctggt cgcccctgat gggcaaaaca
    12001 tctatgatac acgcaattgt ggatcaatat agtcactgtg aatgggtgga aaatagcatg
    12061 agtgccaatg aaaacaacct gatttggatc gatcttgaga tgaccggtct ggatcccgag
    12121 cgcgatcgca ttattgagat tgccacgctg gtgaccgatg ccaatctgaa tattctggca
    12181 gaagggccga ccattgcagt acatcagtcc gatgaacagc tggcgctgat ggatgactgg
    12241 aacgtgcgca cccataccgc cagcgggctg gtagagcgcg tgaaagcgag cacgatgggc
    12301 gatcgggaag ctgaactggc aacgctcgaa ttcttaaaac agtgggtgcc tgcgggaaaa
    12361 tcgccgattt gcggtaacag catcggacag gaccgtcgtt tcctgtttaa atacatgccg
    12421 gagctggaag tctacttcca ctaccgttat ctcgatgtca gcaccctgaa agagctggcg
    12481 cgccgctgga agccggaaat tctggatggt tttaccaagc aggggacgca tcaggcgatg
    12541 gatgatatcc gtgaatcggt ggcggagctg gcttactacc gcgagcattt tatcaagctg
    12601 taaaattttt cctggtcacg taagcgccgg aatccacttg cggcggcgtg attttgacgc
    12661 taaatcgaac gtcttgctga taatttcagc acttgagata aaaacgcaaa aaaaactttt
    12721 ttggggggtt gcagagggaa agatttctcg tataatacgc ctcccgtaac gacgcagaaa
    12781 tgcgaaaatt acgaaagcaa aattaagtag ta
//
LOCUS       DADLSA010000109        12806 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.109, whole genome shotgun sequence.
ACCESSION   DADLSA010000109 DADLSA010000000
VERSION     DADLSA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.109"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(286..492)
                     /locus_tag="JN691_003008"
     CDS             complement(286..492)
                     /locus_tag="JN691_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAY7244399.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            805..882
                     /gene="kdpF"
                     /locus_tag="JN691_003009"
     CDS             805..882
                     /gene="kdpF"
                     /locus_tag="JN691_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009651669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAY7244400.1"
                     /translation="MSAGVLLVFLLLGYLVYALINAEAF"
     gene            882..2531
                     /gene="kdpA"
                     /locus_tag="JN691_003010"
     CDS             882..2531
                     /gene="kdpA"
                     /locus_tag="JN691_003010"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAY7244401.1"
                     /translation="MAAQGFLLIATFLLVLMVLARLINDIPLPGTAGVERILFRLPGV
                     SDHEMNWKQYLCAILGLNMLGLAELFFMLLGQHYLPLNPQQLPGLSWDLALNTAVSFV
                     TNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSMSTLGNAWV
                     DLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGPVASQEAIK
                     MLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGDRRQGRMLL
                     WAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSLFAVVTTAA
                     SCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVFIAGLMIGR
                     TPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGPHGFSEVLY
                     AVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLVSKKSQPAS
                     SGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2554..4601
                     /gene="kdpB"
                     /locus_tag="JN691_003011"
                     /pseudo
     CDS             2554..4601
                     /gene="kdpB"
                     /locus_tag="JN691_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4610..5182
                     /locus_tag="JN691_003012"
     CDS             4610..5182
                     /locus_tag="JN691_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HAY7244402.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5175..7859
                     /gene="kdbD"
                     /locus_tag="JN691_003013"
     CDS             5175..7859
                     /gene="kdbD"
                     /locus_tag="JN691_003013"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HAY7244403.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFSACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVDNALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7856..8533
                     /gene="kdpE"
                     /locus_tag="JN691_003014"
     CDS             7856..8533
                     /gene="kdpE"
                     /locus_tag="JN691_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HAY7244404.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8808..8912
                     /locus_tag="JN691_003015"
     CDS             8808..8912
                     /locus_tag="JN691_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HAY7244405.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9223..11410
                     /gene="speF"
                     /locus_tag="JN691_003016"
                     /pseudo
     CDS             9223..11410
                     /gene="speF"
                     /locus_tag="JN691_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11407..12726
                     /gene="potE"
                     /locus_tag="JN691_003017"
     CDS             11407..12726
                     /gene="potE"
                     /locus_tag="JN691_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HAY7244406.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSTTLYVDSWNPHHAPFFSTVGSSIAMTLWAFLGLESACANTDVVEN
                     SERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
BASE COUNT         2872 a         3187 c         3524 g         3223 t
ORIGIN      
        1 aagggatgac ggctttctca ccttaccctg aagtttcacg aaagtgtgta aggaagtatg
       61 caatctaact ggagatttct gatgatctag tcgcttagag cgacaatgaa ggtgaccgca
      121 tcctgggtag ccctatgaaa ggatactatt gaaacgtgat gctaaaacag tgatgaaatg
      181 aataatacga tggaaacgtc ttccatgtta attatcatgg aagacgttga tgttgaacac
      241 ctaaaccagc cccctgatac gaggctggct taagcgcgca ggcaatcagt aaaaatcacc
      301 ggttgccttt tcggcctgat ccatccacac cggtttatcg ctggttttcg accagacgcg
      361 gtgcaggtag ctgtaaaagc gggcgcggtc tttccagaaa agcatgaaag gcagcgccag
      421 aacgcccgct gcaaccgcat atgtacggcg taaaaagata agccatgcag gatattctct
      481 gtagagttcc atacttttct cccccttttg tatctacccg gtgaatagca ccggaaaaat
      541 gaatttgttt atctgatgaa aatagtaccg ccttttgtgt aattttacta ctcatccgac
      601 cacttatttt tgcttattga tggtttattt acattcacac tgtaattaaa ttacacaaaa
      661 gttaaattaa tactaaccat cagttaaatc atggcctttg ccatttttat acttttttta
      721 caccccgtcc gcagattttt gcgaaatctt tgcagccaga attctactct tccggtatca
      781 cttttaggcc actggaggtg cactgtgagt gcaggcgtgt tgctggtgtt tttattactg
      841 ggttatttgg tttatgccct gatcaatgcg gaggcgttct gatggctgcg caagggttct
      901 tactgattgc cacgttttta ctggtattaa tggtgctggc gcggctgatt aatgacattc
      961 ctcttccagg tacagcgggc gttgaacgta tactctttcg tttacctggc gtctctgacc
     1021 atgagatgaa ctggaagcaa tatctttgtg ccattctcgg cctgaacatg ctggggctgg
     1081 cggagctgtt ttttatgttg ctcggtcagc actatctgcc gcttaatcca cagcagttgc
     1141 cggggctgtc gtgggatctg gcgctgaata ccgccgtcag ctttgtcacg aataccaact
     1201 ggcaatctta tagcggtgaa accacgttga gctatttcag ccagatggca ggcttaacgg
     1261 tacaaaactt tctttctgcc gccagcggga ttgcggtgat ttttgccttc atccgtgcgt
     1321 ttactcgcca gagcatgagc acactcggga atgcctgggt cgatctgcta cgcatcacgt
     1381 tatgggtgct agtccctgtg gcgttgttga tcgcactgtt ttttattcaa caaggtgcgc
     1441 tgcaaaactt tctgccctat caggcagtga ataccgttga aggagcgcaa cagctgttac
     1501 ccatgggacc agtagcttct caggaagcga tcaagatgct cggtactaac ggcggtggct
     1561 tctttaatgc caactcgtcg catccgtttg aaaacccaac cgcactgacc aacttcgtgc
     1621 agatgctggc gatcttcttg atcccaacag cgctgtgctt tgcctttggt gaagtgacgg
     1681 gcgatcgccg ccaggggcgc atgttgctgt gggcgatgtc agtgattttt gtcatctgcg
     1741 taggcgtggt tatgtgggca gaagttcagg gtaatcctca tctgctggcg ctcggcgcag
     1801 acagcagcat caatatggaa ggtaaagaga gccgtttcgg cgtgctggtc agtagcctgt
     1861 ttgcggtcgt gactacggcg gcgtcctgtg gcgcggtgat tgcgatgcat gattcgttta
     1921 ccgctctcgg cggcatggtg ccgatgtggc taatgcaaat tggtgaagtg gtgttcggcg
     1981 gtgtcggttc tggtctttac ggcatgatgc tgtttgtcct gctggcggtg tttattgccg
     2041 ggctgatgat tggtcgtaca ccggaatatc tgggtaaaaa aatcgacgta cgcgagatga
     2101 aactgactgc actggcgatt ctggttactc caacactggt actgatgggc gcggcgttgg
     2161 cgatgatgac cgacgccgga cgtagcgcca tgcttaaccc tggcccgcat ggttttagcg
     2221 aagtgctgta cgccgtgtcg tccgccgcta acaacaacgg cagcgccttt gccggattaa
     2281 gcgccaactc tccgttctgg aactgtttac tggcgttctg catgtttgtc ggtcgcttcg
     2341 gggtgattat cccggtgatg gcaattgccg gttcgctggt gagtaaaaag agccaacccg
     2401 ccagctccgg cacgctgcca acgcacggcc cgctgtttgt tggcctgtta atcggcaccg
     2461 tgttgctggt tggcgcactg acctttatcc ctgccctggc gcttggtccg gtggcggaat
     2521 atctctcctg atgatattga gtgagcactg aatatgagtc gtaaacaact ggcgctattc
     2581 gaaccaacac ttgtcgttca ggcgctgaaa gaagcggtga aaaaattaaa cccgcaggcg
     2641 caatggcgca atccggtgat gtttatcgtc tggatcggca gtctgctgac cacctgtatt
     2701 agcatcgcga tggcaagcgg tgcgatgcat ggcaatgcgc tgtttagcgc ggcaattagc
     2761 ggctggctgt gggtcaccgt actgttcgct aatttcgccg aggcgctggc agaaggccgc
     2821 agtaaagcgc aggccaacag tcttaaaggg gtgaaaaaaa ctgcctttgc ccgcaagctg
     2881 cgtgagccga aatatggcgc tgcggcggac aaagttcctg ccgatcaact gcgtaaaggt
     2941 gacatcgtgc tggtagaagc tggcgatatt atcccctgcg atggtgaagt cattgaaggc
     3001 ggcgcatcgg tcgatgaaag cgccatcacc ggggaatcgg caccggtgat ccgtgaatcc
     3061 ggcggcgatt ttgcctccgt caccggcggc acgcgtattc tttctgactg gctggtgatt
     3121 gagtgtagcg ttaaccccgg tgagacattt ctggatcgga tgatcgcgat ggtggaaggc
     3181 gcacagcgac gcaaaacacc gaacgagatt gccctgacta tcctgctgat tgctctgacc
     3241 atcgtctttt tactggcaac cgccacgctg tggccgtttt ccgcgtgggg cggtaatgcc
     3301 gtgagtgtaa cggtactggt ggcgctgctg gtctgtctga tcccaaccac tattggtggc
     3361 ctgttgtcag cgatcggcgt cgccgggatg agtcggatgc tgggcgcgaa tgtgattgcc
     3421 accagcggac gtgcagttga agcggcaggt gacgttgacg ttctgctact ggataaaacc
     3481 ggcaccatca cactcggtaa ccgtcaggcg tcggagttta tccccgcgca gggcgtggat
     3541 gaaaaaacgc tggctgacgc cgcacaactg gcttcgctgg ctgatgaaac gccggaaggt
     3601 cgcagtattg tgatcctcgc caagcaacgt tttaacctgc gcgagcgcga tgtgcagtcg
     3661 ctccatgcca cctttgtacc gtttactgcg caaagccgga tgagcgggat caacatcgac
     3721 aaccgcatga tccgtaaagg ttctgtcgat gccattcgcc gccatattga ggctaacggc
     3781 ggtcacttcc ctgccgatgt tgatcaaaaa gtcgatcagg ttgcgcgtca aggagccacg
     3841 ccgctggtgg tggtggaagg ttctcgtgtg ctgggcgtta ttgcgctgaa agatatcgtc
     3901 aaaggcggta ttaaagagcg cttcgcccag ctgcgcaaat gggcattaaa acggtgatga
     3961 ttaccggcga taaccgtctg actgccgccg cgatagctgc ggaagcgggt gtcgatgatt
     4021 ttctcgccga agcgacaccg gaggccaagc tggcattgat ccgtcagtat caggcggaag
     4081 gtcgtttggt agcgatgacc ggcgacggca ccaacgatgc tccggcgctg gcgcaggcag
     4141 atgtcgcggt ggcgatgaac tccggcaccc aggcggcgaa agaggcgggc aatatggtgg
     4201 atctcgactc taacccgacc aagttgatcg aggtggtgca cattggcaaa cagatgctga
     4261 tgacccgtgg ctcgctgacc accttcagca ttgccaacga tgtggcgaaa tacttcgcca
     4321 ttattccggc ggcgttcgcg gcaacatatc cgcagttaaa tgcgctgaac atcatgcgcc
     4381 tgcattcgcc cgactccgcc attctcagtg cggtcatttt caacgccttg attatcgtct
     4441 ttttgattcc tctggcgtta aaaggcgtga gttataaacc gcttaccgct tctgccatgt
     4501 tgcgccgtaa cttatggatt tacggtctgg gtgggctgct ggtgccgttt attggtatca
     4561 aagtcattga tttactgcta accgtttgta gtctggtgtg aggtttacca tgagtggatt
     4621 acgtccggca ttatcgacat ttctctttct gttattgatt actggcggcg tttacccgct
     4681 gctgaccacc gcactggggc aatggtggta tccctggcag gccaacggtt cgttgattcg
     4741 tgaaggtgat acggtgcgcg gttcggcatt aatcgggcaa aattttaccg gcaacggcta
     4801 ttttcatggt cgcccgtcgg caacagcaga aatgccctat aatccacagg cttctggcgg
     4861 gagcaatctg gcagtcagta acccggagct ggataaacag atagccgcac gcgttgccgc
     4921 attacgggcc gctaacccgg acgccagcac gaacgttccg gttgaactgg tgacggcgtc
     4981 ggcaagcgga ctggacaata acatcacccc gcaagcggcg gcctggcaga tcccacgcat
     5041 cgcaaaagcg cgtaatctca gcgttgaaca gctcacgcag ctgatcgcaa aatacagcca
     5101 acaaccgctg gtgaaatata tcggccagcc ggtcgtcaac attgtcgaac tcaatctggc
     5161 actggataag cttgatgaat aacgaaccct tacgtcccga ccccgaccgt ctgctggaac
     5221 aaactgccgc gccgcatcgg gggaagctga aagttttctt cagtgcctgt gcaggcgtcg
     5281 ggaagacctg ggcgatgctg gcagaagccc agcgactgcg ggcgcaaggg ctggatattg
     5341 tggttggtgt ggtagaaacc catgggcgga aagataccgc cgccatgctt gaagggctgg
     5401 ctgttctgcc tctgaaacgc caggcgtacc gtgggcggca tatcagcgag tttgatctcg
     5461 atgccgccct cgcccgccgt ccggcgctga tcttaatgga cgaactggcg cacagtaatg
     5521 cgccaggttc tcgtcatcct aaacgctggc aggatatcga agaactgctg gaagctggca
     5581 ttgatgtttt cacaaccgtc aacgttcagc atctggaaag tctgaatgat gtggtcagcg
     5641 gcgtcaccgg aattcaggta cgggaaaccg tgcccgatcc ttttttcgat gccgccgacg
     5701 acgtggtgct ggtggacttg cccccggacg atctgcgcca gcggctgaaa gaaggcaaag
     5761 tctatattgc cgggcaggcg gagcgcgcca ttgaacattt tttccgcaaa ggcaatctga
     5821 tcgccctgcg cgaactggca ctgcgccgta ctgccgatcg cgttgatgag caaatgcgcg
     5881 cctggcgggg gcatcctggc gaagagaaag tctggcacac gcgcgacgcg atccttttat
     5941 gcatcggcca taacaccggc agcgaaaaac tggtccgcgc ggcggcgcgg ctggcgtcac
     6001 ggctggggag cgtctggcac gcggtgtatg ttgaaacccc tgccctgcat cgcttaccgg
     6061 agaaaaaacg tcgggcaatt ctcagcgcct tacgtctggc gcaggagctg ggcgcggaga
     6121 cggcaacact ttctgatcca gcggaagaga aagcggtagt gcgttatgcc cgtgaacata
     6181 atctcggcaa gattattctc ggtcgcccag cctcgcgccg ctggtggcgt cgggaaacat
     6241 ttgctgaccg actggcgcgc atcgcccccg atctcgatca ggtgctggtc gcgcttgatg
     6301 aaccacccgc ccgcacgatt aacaacgcgc cggatagccg ctcatttaaa gacaagtggc
     6361 gggtacagat tcagggatgc gtggttgccg ccgcgttatg cgccgttatc accttaattg
     6421 ccatgcagtg gctgatggcg tttgatgccg ccaacctggt gatgctgtat ctgcttggcg
     6481 tggtggtggt ggcgctattt tatggacgct ggccttcggt ggttgccacc gtcattaatg
     6541 tggtgagttt cgatctcttt tttatcgccc cacgcggcac gctcgccgtc tctgatgtgc
     6601 aatatctgct gaccttcgcg gtgatgttaa ccgtcgggct ggtgatcggg aaccttactg
     6661 ctggcgtgcg ttatcaggcg cgggtagccc gttaccgcga gcaacgcaca cggcacttat
     6721 atgaaatgtc gaaagctctg gcggtgggcc gcagtccgca ggacatcgcc gccaccagcg
     6781 aacaatttat tgcctccacg tttcatgccc gcagtcaggt gttgttgccc gatgacaacg
     6841 gtaaattgca gccgttaaca catccgcaag gaatgacgcc atgggacgat gccatcgcgc
     6901 agtggagttt tgataaaggc ctgcctgcgg gcgcgggcac cgacacgtta cccggtgtac
     6961 cgtaccagat tttgccgcta aaaagcggcg agaaaaccta cgggctggtg gtggtggagc
     7021 cggggaatct gcgccagttg atgatcccgg aacagcagcg cctgctggag acgtttacgc
     7081 tgttagtcga caatgccctt gagcggctga cgctaaccgc cagcgaagaa caggcgcgga
     7141 tggcaagcga acgtgaacag atccgcaacg ccctgctggc agcgctttcg catgatttac
     7201 gcacgccgct tacggtgctg tttggtcagg cagaaatctt aacgctcgat ctggcaagcg
     7261 aaggatcacc ccacgcccgc caggccagcg agattcgtca gcatgtgctg aacactaccc
     7321 gactggtgaa taatctactg gatatggcgc gaattcagtc cggcggcttt aatttgaaga
     7381 aagagtggtt aacgctggaa gaagtagtcg gcagcgcgct gcaaatgctg gaaccgggtt
     7441 tatcgtcgcc catcaatctt tctctgccag aaccgctgac cttaatccac gttgacgggc
     7501 cactctttga acgggtgctg attaatctgc tggagaacgc ggtgaaatat gcgggtgcgc
     7561 aggccgaaat tggtatcgat gcccacgttg agggcgaaaa tctacaactg gatgtctggg
     7621 ataacggccc cggtcttccg ccaggccagg agcagacgat atttgataag tttgctcgcg
     7681 ggaataaaga gtcggcggtg ccgggtgtcg ggcttgggtt ggcaatttgt cgggcgattg
     7741 tggatgtaca cggcggcacc attaccgcgt tcaaccgacc ggaaggtggt gcctgttttc
     7801 gtgttacact tccccagcaa accgctcctg aacttgaaga ctttcatgag gatatgtgac
     7861 aaacgttctg attgttgaag atgaacaggc tattcgtcgc tttctgcgca cggcgctgga
     7921 gggcgacggg atgcgcgtct ttgaggccga aactctgcaa cgcggcttgc tggaagcggc
     7981 aacccgtaag ccagatttga ttattctcga tctcggcctg cccgatggtg atgggattga
     8041 gtttatccgc gacctgcgcc agtggagccc ggtgccggtg attgtgcttt ccgcacgcag
     8101 cgaagagagc gacaagatcg ccgcgctgga tgccggagcg gatgattatc tgagtaagcc
     8161 gtttggcatt ggcgaattgc aggcccgtct gcgcgtcgct ttacgccgcc actccgccac
     8221 cgccgcgccc gatccgctgg tgaaattttc cgatgttacc gtcgatttag ccgcccgcgt
     8281 gattcaccgg ggtgaggaag aggtgcatct cacaccaatt gagttccgcc tgctggcggt
     8341 gctgctcaac aatgccggaa aagtactcac ccagcgccag ctccttaacc aggtgtgggg
     8401 gccaaacgcg gtcgaacaca gtcactattt gcgtatttat atgggacatc tgcgacaaaa
     8461 actggaacag gatccggccc ggcctcgcca cttcattact gaaaccggta ttgggtatcg
     8521 gtttatgctt tgaattgtta ttcaataaat tcgcttaaat agtgattaat ttattattaa
     8581 acctgcgccg aatattatta tttggcaggt ttaatttctt aacgcaaata taaaacacga
     8641 aattacaaca tttaagtaac aaaacagcat ctaatatggc gatattcata attaatgatt
     8701 tattatctgg ggtatgatcg ttttttgttg atcttcttca cagattatag ccatttcatg
     8761 gatagaataa ctctaccttc aactgacaca gcaagcggta aaggtaaatg gaaaataata
     8821 accgcttaat gcctcatata aggcggacaa cccatatcat gaagtttgcc catcgtaata
     8881 gttttgactt tcatttcttt aatgcccgct agtcttctga ctaaagggca ccccaacgta
     8941 caggtctccc tgactttaag cattacaggt taatacctgt attcctcggt gctcatatac
     9001 tgctaaccct tttaaactct aaataattcg agtcgcagca cttgcaactt gaggtatgac
     9061 gagtatagcc agttaccggg ctggtctggg ttattgcatc tgcaaaaagc aaactactga
     9121 tttatttatc agcggtggag ctttgctttt tttccggcgt gatcgatttc tcctttgaga
     9181 aattgaggac ctgctattac ctgaaataaa gagatgaata aaatgtcaga attaaaaatt
     9241 gccgttaacc gttcttgccc ggattgtttt tccactcatc gtgcatgcgt gaatattgac
     9301 gaaagtaatt atattgacgt tgccgccatt attttatcag tcaatgatgt tgaacgtgga
     9361 aaactcgata aaatagacgc tactggctat ggcattcctg tttttattgc aacggaaaat
     9421 gaagaacgtg taccagcaga atatctgcca cgtatttctg gtgtcttcga gcattgcgaa
     9481 tcacggaaag agttttatgg acgtcagtta gaaaccgctg ctagccatta tgaaacgcaa
     9541 ctgcgcccgc ccttcttccg cgcactggtc gattatgtca atcagggcaa tagcgctttt
     9601 gactgcccgg gccatcaggg aggtgagttt ttccgtcgtc atccggcagg gaatcagttc
     9661 gtggagtact tcggagaaac gctgttccgt tccgacttat gcaacgcaga cgtagcaatg
     9721 ggcgatctgc tgattcacga aggcgcgcca tgcattgcac agcaacatgc ggcaaaagtg
     9781 tttaatgccg ataaaacata cttcgttcta aatggcactt catcttctaa caaagtggtt
     9841 ttaaacgccc tgctaacacc gggtgatctg gtgctgtttg atcgcaataa ccacaaatct
     9901 aaccaccacg gagcgttgct acaggctggt gcaacaccgg tttatctgga aacggcacgt
     9961 aacccgtatg gctttatcgg tggcattgat gcgcactgtt ttgaagaaag ttacctgcgt
    10021 gagctgatcg cggaagtcgc accgcagcgg gcaaaagagg ctcgtccttt ccgcctcgct
    10081 gtgattcagt taggcaccta cgacggtacg atttataacg cccgccaggt ggtggataaa
    10141 attggtcatc tgtgtgacta cattctgttt gactccgcat gggttggcta tgagcaattt
    10201 atcccgatga tggctgactg ttcgccgctg ttgctggatc ttaatgagaa cgatccgggt
    10261 attctggtta cgcaatccgt acataaacag caggctggtt tttctcaaac ttcacaaatc
    10321 cataaaaaag acagccacat caaagggcaa cagcgttatg taccgcacaa acgcatgaac
    10381 aacgccttta tgatgcacgc ctccaccagc ccgttctatc cgctgtttgc cgcactggat
    10441 atcaacgcca aaatgcatga aggtgtcagc ggtcgtaata tgtggatgga ttgtgtggta
    10501 aatggcatta atgcccgcaa actgatcctc gataactgtc agcatattcg tccgttcgta
    10561 cctgaactgg tggatggtaa accctggcag tcgtatgaaa cagcgcaaat tgcggttgat
    10621 ctgcgcttct tccagtttgt accaggggaa cactggcatt cttttgaagg ctatgcagag
    10681 aatcaatact ttgtcgatcc ttgcaaactg ttgctgacaa ccccaggtat tgatgcacgt
    10741 aacggcgaat atgaagcgtt cggtgtaccc gcgacgattc ttgctaactt cctgcgtagt
    10801 gccggaaaaa tgcgatctta actccatcct cttcctgctg actccggcag aagatatggc
    10861 caaacttcag caacttgttg ccctgctggt acgcttcgaa aaactgcttg agtccgacgc
    10921 gccattagca gaagtgctgc cttccatcta caaacagcat gaagagcgct acgccggtta
    10981 taccctgcgt cagttgtgcc aggaaatgca tgatttgtat gcccgccaca acgtgaaaca
    11041 actgcaaaaa gagatgttcc gcaaagcgca cttcccacac gtcagcatga atccgcaaga
    11101 agccaactac gcctatttac gcggtgaagt ggagctggtt cgtctgctgg atgccgaagg
    11161 tcgtatcgct gccgaagggg cgcttcctta tcctccgggt gtgctgtgtg ttgttccggg
    11221 tgaaatctgg ggtggtgctg ttttgcgtta cttcagcgct ctggaagaag ggatcaacct
    11281 gctgccaggt tttgcaccgg agctgcaggg tgtctatatc gaagaacatg atggtcgtaa
    11341 gcaagtttgg tgctatgtca tcaagcctcg tgatgcgcaa agcaccctgt tgaaagggga
    11401 aaaattatga gtcaggctaa atcgaacaaa atgggcgtcg ttcagttaac catactgacg
    11461 atggtcaaca tgatgggctc cggtatcatc atgctgccga caaagcttgc cgaagttggg
    11521 acaatttcaa ttatctcctg gctggtgaca gccgtaggct caatggcact ggcatgggca
    11581 ttcgcaaaat gcggtatgtt cagccgtaaa tcaggtggta tgggcggtta tgccgagtat
    11641 gctttcggta aatccggtaa ctttatggcg aactatacct acggcgtctc actgctgatt
    11701 gctaacgtcg cgattgctat ttcggcggtt ggttacggca ccgaactgct cggcgcgagt
    11761 ttgtcgccag tgcagattgg tcttgcgacc atcggggtgc tgtggatttg taccgtggct
    11821 aactttggtg gtgcgcgcat taccgggcaa atcagtagca ttaccgtgtg gggggtcatt
    11881 attccggtgg taggcctgtg cattatcggc tggttctggt ttagcacgac gctttacgtt
    11941 gattcctgga atccgcatca tgcaccgttc ttcagtacag taggttcttc catcgctatg
    12001 acgctgtggg cttttcttgg tctggagtct gcgtgtgcga atactgatgt agtggaaaac
    12061 tcggaacgta atgtgccaat cgcggtactc ggcggtacgt taggtgcggc ggtgatttat
    12121 atcgtctcca ccaacgtgat tgcagggatt gtgcctaata tggagctggc aaattcaacg
    12181 gcaccatttg gcctggcctt cgcacagatg ttcacaccag aagtgggtaa agtcattatg
    12241 gcgctgatgg tgatgtcctg ctgcggttcg ctgcttggct ggcagttcac cattgcccag
    12301 gtgtttaaat cttcatctga tgaaggctac ttccctaaaa ttttctcccg tgtaaccaaa
    12361 gtggatgcac cggtgcaggg aatgttgacc attgtgatta tccagagcgg gttggcgctg
    12421 atgacgatta gcccgtcgct aaacagtcag ttcaacgtgc tggttaacct ggccgtggtg
    12481 accaatatca ttccgtatat tctgtcaatg gcggcattag tcattattca gaaggtcgct
    12541 aatgtgcccc cctcaaaagc gaaagttgca aactttgttg cttttgttgg cgcgatgtat
    12601 agcttttatg cgctgtactc atccggggaa gaagccatgc tgtacggttc catcgtgacc
    12661 ttccttggtt ggacactgta tggtctggta tcaccacgct ttgaactgaa aaataaacac
    12721 ggttaaaaaa gcaaagtaaa aaagggcgat cttgcgacct aatgccagtc agttaagcaa
    12781 ctgactggct ctttttcggg gctgtg
//
LOCUS       DADLSA010000110        12533 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.110, whole genome shotgun sequence.
ACCESSION   DADLSA010000110 DADLSA010000000
VERSION     DADLSA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.110"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(14..337)
                     /locus_tag="JN691_003018"
                     /pseudo
     CDS             complement(14..337)
                     /locus_tag="JN691_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
     gene            520..663
                     /locus_tag="JN691_003019"
     CDS             520..663
                     /locus_tag="JN691_003019"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244407.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(882..2420)
                     /locus_tag="JN691_003020"
     CDS             complement(882..2420)
                     /locus_tag="JN691_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY7244408.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2585..3736)
                     /gene="yiaY"
                     /locus_tag="JN691_003021"
     CDS             complement(2585..3736)
                     /gene="yiaY"
                     /locus_tag="JN691_003021"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HAY7244409.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLLHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            complement(3926..5770)
                     /gene="selB"
                     /locus_tag="JN691_003022"
     CDS             complement(3926..5770)
                     /gene="selB"
                     /locus_tag="JN691_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAY7244410.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLGIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5767..7158)
                     /gene="selA"
                     /locus_tag="JN691_003023"
     CDS             complement(5767..7158)
                     /gene="selA"
                     /locus_tag="JN691_003023"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAY7244411.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQCLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(7256..7864)
                     /locus_tag="JN691_003024"
     CDS             complement(7256..7864)
                     /locus_tag="JN691_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAY7244412.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVLVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFACTKPPKA"
     gene            complement(7935..9020)
                     /locus_tag="JN691_003025"
     CDS             complement(7935..9020)
                     /locus_tag="JN691_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7244413.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHLYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFILEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(9023..9385)
                     /locus_tag="JN691_003026"
     CDS             complement(9023..9385)
                     /locus_tag="JN691_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY7244414.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9922..10605
                     /locus_tag="JN691_003027"
                     /pseudo
     CDS             9922..10605
                     /locus_tag="JN691_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            10607..11485
                     /locus_tag="JN691_003028"
                     /pseudo
     CDS             10607..11485
                     /locus_tag="JN691_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
     gene            11485..12072
                     /gene="mtlR"
                     /locus_tag="JN691_003029"
     CDS             11485..12072
                     /gene="mtlR"
                     /locus_tag="JN691_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAY7244415.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(12084..12293)
                     /locus_tag="JN691_003030"
     CDS             complement(12084..12293)
                     /locus_tag="JN691_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244416.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
BASE COUNT         3071 a         3373 c         3089 g         3000 t
ORIGIN      
        1 aaatccataa gtattactcg gcagaagagg atttgatgtc ggcgagctgt ttctccagcc
       61 cggcaatacg ctgttgtagt tgcacaacgg aggagtcatg gttttacatt ccccatcccc
      121 gctccgggcg gtaaagcgtg gcccaaatcc acaaaaatgg ccagataaca tgcagcgtaa
      181 aaagactcac ccaaccagca acatgaatgg cgtcggcatg aggatgatta cgttttttag
      241 caatcaggta gggaatatca tgtaaaataa taatcccata gaatatcacc agaaatacaa
      301 aaataagcac tcccagtgca aaatagtcca ggaacatatt tccctcaaag aatataaaaa
      361 agaacaatta acgcatatta tgaaatgcca tgatgcagtg caatttcctt atttatataa
      421 cgacatattg ttttttatat actttctgaa atgtgctgtc tgatttttca acaggacgag
      481 gtttatcata ttgaaatagt ggaactattg gccaaactaa tgaataactc cagtaaaaca
      541 tccacagtac agattaagcg tattaaacct tcaattatct accgtttatt gctgattggc
      601 ctcggatcac caatggtgat ttacggcctg gttcgcccgc tcaccatcga aacgcgagat
      661 taaactcctg acaaaacacg ccccagaaac gcacttctgt tggcgtagtc tgggttattg
      721 cgcacgtagg tttaaaacgt aaattgtaac aacgtgcgct ttgtttatgc cggatgcggc
      781 gtgtacgcct tatccggcct acggtcaggt tcccgtaggc atgataagac gcgtaagcgt
      841 cgcatcaggc aatgaatacc caatgcgacc agcttcttat atcagaacag ccccaacggt
      901 ttatccgagt agctcaccag caggcacttg gtttgctggt aatgctccag catcatcttg
      961 tgggtttcgc gaccgatacc tgattgtttg tagccaccaa acgcagcatg tgccgggtaa
     1021 gcgtgataac agttggtcca cacgcgccca gcctgtatgc cgcgccccat cttataggcc
     1081 agattaccgt tgcggctcca gacgcccgcg cccaggccat attgcgtatc gttcgccagc
     1141 tccagcgctt cttccatcgt tttgaaggtg gtcaccgcca gcaccgggcc aaaaatctcc
     1201 tcctggaaca cccgcatatt gttctgacca aacagaatcg tcggttcgag gtagtagccg
     1261 tctttcagtt caccttccag cagcttgcgc cgcccgcctg tgagcacgtc agcgccctct
     1321 tttttaccga tatcaatgta gttgaggatg gtttccagtt gcccgtgaga aacctgcgcg
     1381 cccatttgcg tcacgctgtc gagcgggtta ccgctgcgaa tgctttcgac acggcggatg
     1441 gcgcgttcca taaagcgttc gtagattgac tcctgcacta aggcacgact cggacaggtg
     1501 caaacttcgc cctggttaaa ggcaaacagt gcaaagcctt ccagcgcttt atcgaaaaag
     1561 gcatcttctt catccatcac atcagcaaag aagatatttg gcgacttacc gcccaactcc
     1621 agcgtcaccg gaataatgtt ttgcgttgcg tattgcataa tttgttggcc cacttccgtt
     1681 gagccggtaa acgccacttt ggcgatgcgt ttcgaggtcg ccagatattc gccaatttcc
     1741 ccacctgcgc cattgaccac gttcaccacg cccggcggca gtaaatcacc gacaatttcc
     1801 attagcagca gtacagaaag cggggtaaga cgtgcgggtt tcagcaccac acagttgccc
     1861 gccgccagcg cgggagccat tttccagctc gccatcagca gcgggaagtt ccacgggata
     1921 atctgcccca ccacgcctaa cggttcatgg aaatgatagg ccacggtttc gctatcaact
     1981 tcactgatcc caccttcctg cgcccgaata cacgaggcga aatagcggaa atggtcaatc
     2041 gccagcggta catccgcagc actggtttcg cgaatgggtt tgccgttatc ccaggtttca
     2101 gctgtcgcta acagctcgag gttttgttcc attcgatcgg caatcttaaa cagaatcgcc
     2161 gcgcgatcct gcaccgaggt gtgcgcccat ttatctttca ctttgtgcgc agcatccagc
     2221 gccagatcga tgtctcgttt gcccgaagac gccacttcgc acagcagctg cccggtcacc
     2281 ggcgtcaggt tctggtaata ctcaccatcg gcaggggcta cccattcgcc gccaataaag
     2341 ttgtcgtagc gggcttttaa cttgatgggg aaaccatact cgccgggctt aatctgtgct
     2401 gaagggggat tattggtcat gatcgtctcc ttgcggtgtg aggtataaca agggtagacg
     2461 tgactggcga aatattcgcc agtcggtaac agctttacga cagctatcac gaatttacgg
     2521 gcaaggatta cagagatagt tgaaaaagcg gctgacaatt tgccagccgt tgttggaatg
     2581 atgattacat tgcggcgcga taaatcgcca caatttcttc gtgagttgcc tggatcgggt
     2641 tagtaaagcc acaggcatct ttcagggcat tagtcgccag aaccgcgaaa tcttcttctt
     2701 tcacgttcag gtcgcgtagg cctgccggga tatccacttt cttcgccagt tcacggatgg
     2761 cgttaatgca ggcttcagca ccttccgcgt cgtttttacc tgtcacgttc acgcccattg
     2821 cagcggcaca gtcacgcaga cgtgcagcgg cgactttgct gttgaatacc tgaacatgca
     2881 gcagcagaac ggcgttacat acaccgtgtg gcaggttgta gaaaccgccc agctggtgcg
     2941 ccatcgcatg aacataaccc agagaagcat tattgaacgc cataccggca aggaactggg
     3001 cataagccat tgcttcacgc gctttcgcat tactgccatc ttcaacggct aacggcaggt
     3061 tttcggcaat catggtcacg gctttcagtg cacaagcgtc agtgatcggc gtggcggcaa
     3121 tagaaacata cgcttcgata gcgtgcgtta aggcatccat acccgttgcg gcggtcagtg
     3181 acttcggcat accaatcatc agagaggagt cattgacaga aagcagcgga gtcacgtgct
     3241 tatcaacaat cgccattttg ttatgacgcg cttcgtcagt gatgatgcag aaacgggtca
     3301 tttcagaggc cgtacccgcc gtggtattga tggcgatcat cggcagctgc ggttttgcag
     3361 agcggtcaac gccttcgtaa tcgcgaatat cgccgccatt ggctgccacc agcgcaatac
     3421 cttttgcgca gtcgtgtgga gaaccaccgc ctaaggagat cacgctatcg caattatttt
     3481 ctttaagtaa tttcaaacct gcggcgacgt tttccgtggt ggggttaggt tgggtgccat
     3541 cataaataac gctaaaaata ttgcgttctt ccagtgcttt ttgcacatca cccgccatac
     3601 ctaatttcgt taacatattg tcagtgacaa ttaaggtacg ggtaaatcca taatctgcca
     3661 tcatattcat tgcatcagtc aatgaatcag cgccgatgac attcactgaa ggaataaaga
     3721 aagttgaaga tgccataaca cactcctgaa agtgtaaaaa agggtgggca gcttactgag
     3781 gattttcatt cgaaaacatg atcattatta ttaattatca aaattaatct aataaaaagc
     3841 acattgtatt cattaaacaa tgtgctttat ttaaaatcat aagaaatttg tattaattta
     3901 ttttaaaaat taattaatca tttccttatt tttccggaaa taataatgcg tcgcgcaata
     3961 aatgatcatt tccacgacga cgcgtaaagc caatgcggtc aaaatattcc agaatctgaa
     4021 ttcccagctt tcggcctacg cctaagcgat cgcggaaatc cgccgcgcag gttgaaccac
     4081 actcctgatc gagatcgcgg atcatattgg caaattcgac aatccgatcg ttacggtaat
     4141 aacgatcttt gacgatcgcg gtaattattc cttgctgcgc cgcctggcgt agcgtcaggc
     4201 gcattgcctg ctcgtcggta cccgtctctt ttgccagatc acgcacccac cacggctcgt
     4261 caccaaacag cggctctgct ttttgccaga tggcctgctg ctcttcgctg aagcccgctt
     4321 tgtgatccgg cagatgcagc caaccgtgat gactgaggat gtcgccgctt tcgcgcatct
     4381 tttcaatcag caacagtact agcgcttcat cttccattgg caacgccata cgtcgcagac
     4441 gttcgcgccc aggcccaggt tcatcgcgat gttgctcatg ataagtcgct aatgtgtcga
     4501 gaattttccg ttgccagcga gcggcaaccg gcgcattcaa caagctataa ccagcctgaa
     4561 tatagccagg ctgttgcagc agttcgcgca tcccttcgcc gttgagctgg cgcgcccagg
     4621 cgaaatcagg aagattaacc gcgccgcgtt gcaggtgaac ggctaacgct tcgctatcat
     4681 tctgtgccct ggcgagggaa gccagccatt gcagatactc tggtttacgt ttaccgcgac
     4741 gcggcgggtt aagcatcacg acgcgcgctc cggccagcgt gttgcgggca gagatatcgc
     4801 gcaataccag gcggtcgtta tctgccagcc ataacggggt gtcgaagacc agttcagcaa
     4861 ggttatcttc cagcagtgaa acgcgtcccg tgacgtggct ggcggcgtgg tgaatatgca
     4921 gcggctgcca ctgggtcagc ggtgtatggg tttgaagctc gacaatcacc cgtgtgaacg
     4981 gctctggcgg cacatcggca agcagccagt cgccacggtt aatctgctct ttttccgcat
     5041 cacccgcgat gtcaagcgcg atacgctgcc cggcattggc ggtttctgtt ggctggtttt
     5101 gcgcatgcag cgcacgtaca cgcatcggtt tatttacacc agtcagccag agtgaatcgc
     5161 ctaccttcac ttccccgctt aacgccgtac cggtgacgac cagcccggca ccttttacgg
     5221 taaatgcgcg gtcaatcgcg aggcggaaac tatgttggct ggcgtgctcg cgttccggca
     5281 actgaagcag atgctcgcgc agggcatcaa ttccccgacc ttcggttgct gcggtgataa
     5341 acagttttgc ctcagcaaaa ccgtattccc gcagaacctc ctttacctgg cgttcaacct
     5401 catcaacacg cgcttcgtcc acgcgatcgg ctttggtcag cgccactgtc agcatcgggt
     5461 taccggtcag ctgcaaaatc gccagatgct cacgggtctg tgccatcacg ccgtcatcgc
     5521 acgccaccac caacagcgcg tgatcgatac caccaacgcc cgccagcatg ttggaaagaa
     5581 acttttcatg accgggaacg tcgataaaac caggcacgcg gccatccggc tgcggccagt
     5641 aggcatagcc gagatcgatg gtcatgccgc gctttttttc ttccggcaga cggtcagcat
     5701 ttacgccagt aatcgcctgc aataaggttg ttttgccgtg gtcaacgtgt ccggcagtcg
     5761 caataatcat ttcaacaaca tctccaaaaa ccgttgctca tcttcaaggc agcgtaaatc
     5821 cagccacaat cgtccgtcat aaatacgacc aatcaccggc actggcaatt cacgccagcg
     5881 ggcggctaat gactcaaggt ggctaccgcg tccatcatgg ggtgtaaacg ttaatgccgc
     5941 gctcggcagg cgatcaaccg gcagcgaacc actgccaatc tgcgaaagac atggcataac
     6001 ctgtaccgca aactccgcgc cgtaatgtgc ggcaagggga gcctgtaaac attgtgcctg
     6061 gatttgaatg acctctgcgc tgcgggtaag caggcgcagg gtcggtaatt tttcactcag
     6121 agcttcaggg tgtaaataaa gacgcaacgt ggcttccagc gccgcgaggg tcattttatc
     6181 cgcgcgtaat gcacgcttca gcgggtggct ttgcaggcgg gcgatcatct cttttttacc
     6241 aacgataatc cctgcctgcg gcccgcctaa caacttgtcg ccggagaagc tcaccagact
     6301 gacgcccgcc gcaatcaact cctgcggcat tggctctttc ggcaaaccgt actggctaag
     6361 atcgaccagc gagccactgc ctaaatcagt caccactgga acatccagct ctttgccgag
     6421 cgccaccagt tcagcttcgt ctatcgcttt ggtgaacccc tgaatgctgt agttactggt
     6481 atgtactttc atcaacagtg cggtattttc attcaccgcc tgacgataat cattcgcgtg
     6541 cgtgcggttg gtggtcccta cttcgtgtag ggtacagcct gcctgacgca taacatcggg
     6601 aatacgaaac gcgccgccaa tctccaccag ttcgccgcga gataccacca cctctttccc
     6661 gctggcagtg gccgccaaca tcaataacac cgccgccgca ttgttattga cgatacaggc
     6721 atcttccgcc cccgtaatac ggcacagcag ctgcgccagc gcccgatcgc gatgtccgcg
     6781 tccggcgtcg tccagatcgt actcgagggt cactggcgaa cgcatagcct gcgcaacggc
     6841 ttccaccgct gcttccgcct gtaaagctcg cccaaggttg gtatgcagca cggttcccgt
     6901 caggttgatc accggacgca gcgcgctctg cgcctctttc gtcaaccggg catcgacttc
     6961 ttgcgcccag ttttcacacc acgcaggcag cgtctggctg ccacgaatca cttctcgcgc
     7021 ttcgtcgagc atctgacgca acaattccac cacgcgggtg tgaccataag tatcacgcaa
     7081 agaaaggaag gagctatcgc gcaataagcg atcaatagcc ggaagttgac tatagaggga
     7141 acgcgtttcg gttgtcatag gaaacctggc tgatcaaggc cctctcacac ggagaagggc
     7201 gtttaacata accacggatt gtaacgtgag atgggtcagg aggacatatc gcgcatcaag
     7261 cctttggcgg tttggtgcaa gcaaaacttt cgcggctaaa caggttttcg accagtttga
     7321 ctaagtgtgg gcggtcgaca caccaacctg gcgcaacgcg gcggaaattg agatatccga
     7381 cagcacaggc aatggcgata gtcgccagat tgaccgtatc ggttttgagt gtgccatcga
     7441 ccagatatcc ttccagcaca tccagactgc ggttgatttt ctcccgctgg cgtaacaatt
     7501 catcttcaga ctgctgcgcc gccggacgcg cctgttcacg caccgatacc agcccggcat
     7561 ccataatgcc atccgccagc gcctcaattt tgcgcacccg caacgactcc agcggatcgc
     7621 gcggcaacat cgccggagcg acattcatta actcaatata ttcagcgatg atcggcgaat
     7681 caaaccagca ttcgccctct tcggtcacca gcaccagcac tttccctaac gggttaaatt
     7741 gcgccacgcc gttatccgcg ttatagggca gttcattaat aaattcgaaa gttatgccct
     7801 tttctaacaa cagaatagaa agtttgcgta caaacgggct ggtgtagcta ccgacgagtt
     7861 tcatgccgag tcctttgtgc gaggaaaaat atcagtatgg ctcaggctca cgcaaaacac
     7921 aggtgatata agtctcaatg atccaaataa agataatgca tccaactggt cattcttagc
     7981 agcactttcc gcatcaccga aacatggctg aaatggtcgg agtaaaccgc cacctgggcg
     8041 tagatgccgt cgggtaaggc atcgatatca tcgttagggt ccagttcaat ggttcccagc
     8101 acaccgtccg tgccgggaac gaccgttaat gattgcaata ccccctgcgc ctgataagaa
     8161 ccgcctggca cgacaggtaa aatactagtc aatttgccgt ggaacacctg cccaggtagc
     8221 gcgttaaaca ccacttccgc atcatcgcca ggtttcagac gtaacagcga gttttgccga
     8281 aattgggcga caatttgccg tttttgctcg aggatgaaga ccatcaccgg acgcagcggc
     8341 aaggcagctg cgtatgtacc tgggcggatc agtacctgag tgacgtagcc attgcttggt
     8401 gcgcgaatga cagtctgctc aagattatat tttgcttcag taagttgcgc tcttaagctc
     8461 acaatctgcg attgctcgcc gttgaccata ctgtcgagct gactctggat ctgcgcctgc
     8521 tccgccaccg agcctttcac taacgcatcc tgcgcgagga aattttgccg cgcatcgtcg
     8581 atgtcacgtt ccgagaacgg attcaccgcc gcctggctgc ctttcaggta acgttgataa
     8641 tttttaaaca gacggtcgcg ctccgctgaa acctgggtgg tgttggcctg cgcttcagtg
     8701 agctgcgcac gcagcgtctt tatattatgc gtcgccgtca tcaggtcagc ctgaagtctg
     8761 tcaactcgcg cctggtaacg aaccgggtcg agcttaaaaa gcacctcgcc cttttgaata
     8821 agctgattat tcttgtcagt gacttcagta acaattcccg tcacctgtgg cgtgataggg
     8881 atcgctatca ctgccttttg cgcggtaaaa gtgtaaaggt ggttgtagtt catcaacaaa
     8941 atcaaaccac tcaccagaaa cacgcctccc agcgccgccg tcgccagcgc cacgtaagtt
     9001 aaaacaatca atagatccat aattagatct ccgggaacgt ggaatagtca gtctttttct
     9061 cggcggcgag ttgatgctcc agccgggaaa ttcggtcaga aagtgcggcg atttccggtt
     9121 cagtcgcttt ctcctgcgac gcaacatgtg actgcatacc ccagccacgc tccggttgat
     9181 agagcgtcgc ccagatccac agaaacggcc agataacatg cagagtaaac aggctcaccc
     9241 agcccgccgt atgaatagcg tcggcatggg ggtgattgcg ctttttggca atcagataag
     9301 ggatgtcatg tatcgcgatg attccataaa aaatcaccag aaagacgaag atcagcactc
     9361 ccaacgcgaa atagtttaga aacatatctg cctcggattc acgtttatca gtgttgtttt
     9421 ttggctggca gccagaaggg agtcaggctg atattttgac aataatccgg gttcgcgatt
     9481 ctcgccataa caccaaagaa taatttttag aggtgatgag ttgcttagtt acataacgat
     9541 tgtatgacga aggcataaca tgctgtagat cacatcaggt gaacgccgta agaaaatatc
     9601 ttgtgattca gatcacaaag attcaacaaa ccatcaaaac aaaaatgtga cattactcac
     9661 atttaaatgc catttttagc gaaaatcacc gccttgtttc ttttttacac aggcgttttg
     9721 tgatgagtgt cacgtcaatt acctctctac cccctatatt tatgtgactg atatcacaca
     9781 aaagcccgtc gactggacag ttaaccgatt cagtgccaga tttcgcagta tctacaaggt
     9841 ccggctacct ctgccgccac attaacaaaa aacctcgggc ttccagcctg cgcgacagca
     9901 aacataagaa ggggtgtttt tatgtcatcc gatattaaga tcaaagtgca aagctttggt
     9961 cgtttcctca gcaacatggt gatgccaaat atcggcgcgt ttatcgcgtg gggtatcatc
    10021 accgcgttat ttattccaac agggtggtta ccgaacgaga cgctggcgaa gctggtcggg
    10081 ccgatgatca cttatctcct accgctgctg atcggttata ccggtggtaa gctggtaggc
    10141 ggcgaacgtg gcggcgtagt cggtgccatt accaccatgg gcgttatcgt cggcgcagac
    10201 atgccgatgt tcctcggttc tatgattgca ggtccgctgg gcggctggtg cattaagcac
    10261 ttcgaccgct gggtagacgg taagatcaaa tccggttttg agatgctggt gaataacttc
    10321 tccgcaggca tcatcgggat gatcctcgct attctggcat tcctcggcat tggcccgatt
    10381 gttgaagccc tgtccaaaat gctggctgcg ggcgttaact tcatggttgt ccatgacatg
    10441 ctgccgctgg cgtctatctt tgttgaaccg gcgaaaatcc tgttcctcaa caacgccatt
    10501 aaccacggta tcttctcgcc gctgggtatt cagcagtccc atgaactggg taaatcaatc
    10561 ttcttcctga ttgaagctaa cccaggtcca ggtatgggcg tgctttgtgc tggaacgtat
    10621 tgctccggct atcgccaaag ggctggtgaa acgcaaagaa caaggtaatg aatccccgct
    10681 gaacatcatc gcctgtgaaa acatggtacg cggcaccacg cagctgaaag gtcatgtgat
    10741 gaacgccctg ccggaagacg ccaaagcgtg ggtagaagag cacgttggct ttgtcgattc
    10801 cgccgttgac cgcatcgtac cgccttcggc ttcggcaact aacgatccgc tggaagtgac
    10861 ggtagaaact ttcagcgaat ggattgtcga taaaacgcag ttcaaaggcg cactgccgaa
    10921 catcccaggc atggagttaa ccgacaacct gatggcattt gtcgaacgta aactcttcac
    10981 cctgaacacg ggtcatgcta taaccgcgta cctcggaaaa ctggccggtc atcagaccat
    11041 tcgtgacgct attctcgacg agaaaatccg cgcggtggta aaaggtgcga tggaagaaag
    11101 tggtgcggta ctgatcaagc gctacggctt tgacgcagac aaacatgcgg cgtacatcca
    11161 gaaaatcctc ggtcgttttg agaacccgta tctgaaagat gatgtagagc gcgtaggccg
    11221 tcagccgctg cgtaaactga gtgctggcga ccgtctgatc aagccactgc tcggtacgct
    11281 ggaatacagc cttccgcaca agaatctgat tcaggggatt gctggtgcaa tgcacttccg
    11341 cagtgaagat gatccgcagg ctcaggaact ggcagcactg atcgctgaca aaggtccgca
    11401 ggcggcgctg gcacagattt ccggtcttga tgccaacagc gaggttgtat ccgaggcggt
    11461 aaccgcttat aaagcaatgc aataatggtg gaccaggcgc aggacaccct gcgcccgaat
    11521 aacagattgt cagatatgca ggcaacaatg gaacaaaccc aggcctttga aaaccgtgtg
    11581 cttgagcgtc tgaatgctgg caaaaccgtg cgaagctttc tgatcaccgc cgtcgagctt
    11641 ttgaccgagg cggtaaatct tctggtgctt caggtattcc gcaaagacga ttacgcggtg
    11701 aagtatgctg tagaaccgtt actcgacggc gacggtccgc tgggcgatct ttctgtgcgt
    11761 ttaaaactca tttacgggtt gggcgtcatt aaccgccagg aatacgaaga tgcggaactg
    11821 ctgatggcat tgcgtgaaga gctaaatcac gacggcaacg agtacgcctt taccgacgac
    11881 gaaatccttg gaccctttgg tgaactgcac tgcgtggcgg cgttaccacc gccgccacag
    11941 tttgaaccag cagactccag tttgtatgca atgcaaattc agcgctatca acaggctgtg
    12001 cgatcaacaa tggtcctttc actgactgag ctgatttcca aaatcagctt aaaaaaagcc
    12061 tttcaaaagt aagcaacgtc tgcttactgc ccctctacct gcttcggccg ataaagccga
    12121 cgataatact ccagacgttg tagatataac ggcacgcttt catcgggtat tccggacgga
    12181 atcgcgttac ggggaggaag ttttttcaga tactcccgga acgcctggct tgatgccatg
    12241 aaatctacgg ctttatcgat aagaagcgga acgttttcac ctaatttgcc catgatgata
    12301 tctccgtatt accccgcgcc gggaatgcgc ggccgccaat tttagtttag gttcccggta
    12361 aagatgacat taaggaaaac gtgctgaatc ctcaataatc atccgctgct gtttattatc
    12421 tttttctata gatttcttaa ttaatcaacg aattgcgtag tttttgagca tgttaatgat
    12481 tacgttgcag aactattaca atgtcatcag ttgtgaacaa agcacctggt cgc
//
LOCUS       DADLSA010000111        12529 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.111, whole genome shotgun sequence.
ACCESSION   DADLSA010000111 DADLSA010000000
VERSION     DADLSA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.111"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..379
                     /locus_tag="JN691_003031"
     CDS             <1..379
                     /locus_tag="JN691_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708817.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY7244417.1"
                     /translation="QRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAP
                     TADVAKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTP
                     RYDKSAADLAWQRTLKWFDKYLS"
     gene            498..785
                     /locus_tag="JN691_003032"
     CDS             498..785
                     /locus_tag="JN691_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAY7244418.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            974..2092
                     /gene="hybO"
                     /locus_tag="JN691_003033"
     CDS             974..2092
                     /gene="hybO"
                     /locus_tag="JN691_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAY7244419.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            2095..3081
                     /gene="hybA"
                     /locus_tag="JN691_003034"
     CDS             2095..3081
                     /gene="hybA"
                     /locus_tag="JN691_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAY7244420.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            3071..4249
                     /gene="hybB"
                     /locus_tag="JN691_003035"
     CDS             3071..4249
                     /gene="hybB"
                     /locus_tag="JN691_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAY7244421.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            4246..5949
                     /gene="hybC"
                     /locus_tag="JN691_003036"
     CDS             4246..5949
                     /gene="hybC"
                     /locus_tag="JN691_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAY7244422.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            5949..6443
                     /locus_tag="JN691_003037"
     CDS             5949..6443
                     /locus_tag="JN691_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAY7244423.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            6436..6924
                     /gene="hybE"
                     /locus_tag="JN691_003038"
     CDS             6436..6924
                     /gene="hybE"
                     /locus_tag="JN691_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAY7244424.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMYDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            6917..7258
                     /gene="hypA"
                     /locus_tag="JN691_003039"
     CDS             6917..7258
                     /gene="hypA"
                     /locus_tag="JN691_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY7244425.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCRGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            7392..7640
                     /gene="hybG"
                     /locus_tag="JN691_003040"
     CDS             7392..7640
                     /gene="hybG"
                     /locus_tag="JN691_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAY7244426.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(7763..8629)
                     /gene="yghU"
                     /locus_tag="JN691_003041"
     CDS             complement(7763..8629)
                     /gene="yghU"
                     /locus_tag="JN691_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAY7244427.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            8834..10693
                     /gene="gss"
                     /locus_tag="JN691_003042"
     CDS             8834..10693
                     /gene="gss"
                     /locus_tag="JN691_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAY7244428.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            10985..12484
                     /gene="pitB"
                     /locus_tag="JN691_003043"
     CDS             10985..12484
                     /gene="pitB"
                     /locus_tag="JN691_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HAY7244429.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRSFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT         2899 a         3109 c         3449 g         3072 t
ORIGIN      
        1 gcaacgctat ccgcaagcga caggtaaagt gggtattacc ggattttgct atggcggtgg
       61 cgtatcgaac gcggcggctg tcgcgtatcc ggaactggcc tgcgcggtgc cgttttatgg
      121 tcgtcaggca cccactgccg atgtggcgaa gattgaagca cctttactac tccacttcgc
      181 ggaactggac acccgaatca acgagggctg gcctccttac gaggcggcgt tgaaagacaa
      241 taataaggtt tatgaggctt atatctatcc gggggttaat cacggattcc ataatgattc
      301 cacgccccgt tatgacaaat ctgccgccga tcttgcctgg caaaggacac tgaaatggtt
      361 cgataaatat ctctcctgat aggtttatct cttacgggat tacgtcttaa acaagtatga
      421 aaaaatagcg tgcgcaaaag tcgttctttg cctaaaatat cgttatatat ggcgatatat
      481 aacgaatgag gtgaacgatg aataaccatt ttggtaaagg cttaatggcg ggattaaaag
      541 caacgcatgc cgacagtgcg gttaatgtga caaaattctg tgccgattat aaacgcggtt
      601 ttgtattagg ctactcacac cggatgtacg aaaagaccgg agatcgccag cttagcgcct
      661 gggaagcggg tattctgacg cgccgctatg gactggataa agagatggta atggatttct
      721 ttcgtgagaa taattcctgt tctacgttgc gcttttttat ggccggttat cgcctcgaaa
      781 attgatcaaa catacgtatt atcttgcttt aattaattac actaatgctt cttcccttcg
      841 ttttagcgcc ccgccgcagt atcatgatat cgataaccat aataaatgtg tggtaaatgg
      901 cgcatcgatc gcattattga ttttgcgatt gaggcaaaat atatgccagg tcttcgcaac
      961 ggaataacta taaatgactg gagataacac cctcatccat tctcacggca ttaaccgtcg
     1021 tgatttcatg aagctttgtg cagcattagc cgccaccatg gggttaagta gcaaagccgc
     1081 tgcagagatg gccgaatcgg ttactaaccc gcagcgtccg ccagttatct ggattggcgc
     1141 gcaggagtgc accggttgta cggaatctct gcttcgtgca acgcatccaa cggtagaaaa
     1201 cctcgtgctg gagactatct ctctggaata tcacgaagtg ctttccgccg ccttcggtca
     1261 tcaggtcgaa gagaacaaac ataacgctct cgagaagtac aaagggcagt atgtgttagt
     1321 ggtggatggt tccatcccat taaaagataa cggtatttat tgcatggttg ccggtgagcc
     1381 gattgtggat cacatccgca aagcggcgga aggcgcagca gccattatcg ctattggttc
     1441 ctgctccgcg tggggcggtg ttgccgcagc tggagttaac ccaactggcg cagtcagctt
     1501 gcaagaagtt ctgccaggca aaaccgttat caatattccg ggctgcccgc cgaacccgca
     1561 caacttcctc gcgaccgttg cgcacatcat cacttacggc aaaccgccga aactggatga
     1621 caaaaaccgt ccgaccttcg cctatggccg tctgattcac gaacactgcg aacgtcgccc
     1681 gcacttcgat gctggtcgtt ttgccaaaga gttcggtgat gaaggccacc gcgaaggctg
     1741 gtgcctgtac cacctcggct gtaaagggcc agaaacttac ggcaactgct caacgctgca
     1801 attctgcgat gttggcggtg tgtggccggt ggcgattggt cacccgtgct atggctgtaa
     1861 cgaaaaaggt atcggcttcc ataaaggcat ccatcagctt gccaacgtcg aaaatcagac
     1921 tccgcgttca cagaaaccgg atgttaacgc taaagagggc ggcaacgtct ctgcaggcgc
     1981 tattggtttg ctcggcggtg tggttgggct ggttgccggt gtcagcgtga tggcggtgcg
     2041 tgaactgggt cgtcagcaaa agaaagataa cgctgactca cggggagaat aaccgtgaac
     2101 agacgtaatt ttattaaagc agcctcctgc ggggcattgc tgacgggcgc gctgccgtct
     2161 gtcagtcatg cggctgctga aaaccgcccg ccaattccgg gatcgctggg gatgttgtac
     2221 gactcgacct tgtgcgtagg ctgccaggct tgcgtcacca agtgtcagga tatcaacttc
     2281 cctgaacgta acccgcaagg ggaacagacc tggtcgaaca acgacaaact gtcgccgtat
     2341 accaataata tcattcaggt gtggaccagc ggcacagggg tcaacaaaga ccaggaggag
     2401 aacggctacg cgtacattaa gaaacagtgt atgcactgcg tcgatccgaa ctgtgtctct
     2461 gtgtgcccgg tctctgcact gaaaaaagat ccgaaaaccg gcattgtcca ttacgacaaa
     2521 gacgtgtgca ccggttgccg ttactgcatg gtcgcctgtc cgtacaacgt gccgaagtac
     2581 gactacaaca acccgtttgg tgcgctgcat aagtgcgagc tgtgcaacca gaaaggtgtg
     2641 gaacgtctcg ataaaggcgg tctgcctggc tgcgtagaag tgtgcccggc gggcgcggtg
     2701 attttcggta cgcgtgaaga gctgatggcg gaggcgaaaa aacgtctggc gctgaagcct
     2761 ggcagcgaat accactatcc gcgtcagacg ctgaaatctg gcgacactta cctgcatacg
     2821 gtgccgaaat attatccgca tctgtacggc gagaaagagg gcggcggtac tcaggttctg
     2881 gtactgacgg gtgtgcctta tgaaaatctc gacctgccga aactggacga tctttctacc
     2941 ggtgcgcgtt ccgaaaatat tcaacacacc ctgtataaag gcatgatgct accactggct
     3001 gtgctggcgg gcttaaccgt gctggttcgt cgcaacacca aaaacgacca tcacgacgga
     3061 ggagacgatc atgagtcatg atccacaacc gctgggcggc aaaatcatca gtaaaccggt
     3121 catgattttt ggaccgttaa tcgtcatctg tatgctcctg attgtgaagc gtctggtgtt
     3181 cggtctgggc tctgtctctg acctgaacgg cggcttcccg tggggcgtgt ggatcgcgtt
     3241 tgacctgctg attggcaccg gctttgcctg tggcggctgg gcgctggcgt gggcggtata
     3301 cgtctttaac cgtgggcaat accatccgct ggtgcgtccg gcgctgttgg cgagtctgtt
     3361 tggttactca ctgggtggct tgtcgatcac tatcgacgtg ggtcgctact ggaacctgcc
     3421 gtacttctac attccgggtc acttcaacgt gaactcggta ctgttcgaga cggcggtctg
     3481 tatgaccatc tacatcggcg tgatggctct ggagtttgct ccggcactgt ttgaacgtct
     3541 gggctggaag gtgtcgctac aacgtctgaa caaggtgatg ttcttcatta tcgcgctcgg
     3601 tgcgctgctg ccgaccatgc accagtcttc aatggggtcg ctgatgatct cggcgggcta
     3661 caaggtgcat ccgctctggc agagctatga aatgttgccg ctgttctcgc tgctgacggc
     3721 gttcatcatg ggcttctcga ttgtcatctt tgaaggttca ctggtgcagg cgggtctgcg
     3781 tggcaacggt ccggatgaaa agagcctgtt cgtcaagctg accaacacca tcagtgtgtt
     3841 gctggcgatt ttcatcgggc tgcgctttgg cgagcttatc tatcgcgaca agctgtcgtt
     3901 agcgtttgcc ggtgacttct actccgtgat gttctggatt gaagtcctgc tgatgctctt
     3961 cccgctggtc gttctgcgtg tggcgaagct gcgtaatgat tcccgcatgc tgttcctgtc
     4021 agcactgagc gcactgttag gttgtgcaac ctggcgtctg acctattcgt tggtggcatt
     4081 caacccgggc ggcggttacg cctacttccc aacctgggaa gaactgttga tttctattgg
     4141 ttttgtggct attgagattt gcgcttacat cgtactcatt cgtctactgc cgatacttcc
     4201 tcctttaaaa caaaacgatc ataatcgtca tgaggcgagc aaagcatgag ccagagaatt
     4261 actattgatc cggtaacccg tattgagggg catttacgca tcgattgcga aatcgaaaat
     4321 ggcgtcgttt cgaaagcatg ggcttccggt accatgtggc gcggcatgga agagatcgtg
     4381 aaaaaccgcg atccgcgcga tgcatggatg attgtgcaac gtatctgtgg cgtatgtact
     4441 accactcacg cgctgtcttc cgttcgtgcg gcagaaagtg cgctgaatat cgacgttccg
     4501 gttaacgcgc aatacatccg taacatcatt ctggctgcgc acaccacgca tgaccatatc
     4561 gttcatttct atcagctttc ggcgctggac tgggtggaca tcacttctgc actgcaagct
     4621 gacccaacca aagcctccga aatgctgaaa ggcgtttcga cctggcatct gaacagtccg
     4681 gaagagttca ctaaagttca gaacaagatc aaagatctgg ttgctagcgg tcagttgggt
     4741 attttcgcca atggctactg gggtcacccg gcgatgaaac tgccgccgga agtgaacctg
     4801 attgcggtag cgcactacct gcaggcgctg gagtgccagc gtgacgctaa ccgcgtcgtg
     4861 gcgctgctgg gcggtaaaac gccgcacatt cagaacctgg cagtgggtgg tgtcgcgaac
     4921 ccaatcaacc tcgacgggtt gggcgtgttg aaccttgagc gcctgatgta catcaagtct
     4981 ttcatcgaca aactgagcga ctttgttgag caggtttata aggttgatac cgcggttatc
     5041 gccgcgttct acccggaatg gctgacgcgc ggtaaaggtg cggtgaacta cctgagcgtg
     5101 ccggaattcc cgaccgacag caaaaacggc agcttcctgt tcccgggcgg ctacattgag
     5161 aatgcggatc tgtcctcgta tcgtccgatc acttctcatt ccgatgaata cctgatcaaa
     5221 ggcattcagg aaagcgcgaa gcactcctgg tataaagacg aagcgccaca ggcgccgtgg
     5281 gaaggtacca ccattccggc ttatgatggt tggtctgacg acgggaaata ttcctgggtg
     5341 aaatcaccga ctttctacgg caaaacggta gaagtgggtc cgctggctaa tatgctggtg
     5401 aaactggcgg caggtcgcga atctacccag aacaaactga atgaaatcgt tgcgatttat
     5461 cagaaactga ctggcaacac gctggaagtg gcacagctgc actccacgct gggccgtatt
     5521 attggtcgta ccgttcactg ctgtgaattg caggatatcc tgcaaaacca atacagtgca
     5581 ctgatcacca atatcggcaa aggcgatcac accacctttg tgaagccgaa cattccggca
     5641 acgggtgagt tcaaaggtgt tggcttcctc gaagcgccgc gcggtatgct ctctcactgg
     5701 atggttatta aagacggtat catcagcaac taccaggcgg ttgttccatc aacctggaac
     5761 tctggtccgc gtaacttcaa tgatgacgtc ggtccttacg agcagtcgct ggtgggtaca
     5821 ccggttgccg atccgaataa accgctggaa gtggtgcgta ccattcactc cttcgacccg
     5881 tgcatggcct gtgcggtaca cgtagtggat gccgacggca acgaagtggt ttcagtgaag
     5941 gttctgtaat gcgtatttta gtcttagggg tcggcaatat tttgctgacc gatgaagcca
     6001 tcggcgtgcg gattgtcgaa gcgttagagc aacgatacat tctgccggat tatgttgaga
     6061 tcctcgatgg cggcacggcg ggaatggagc tgcttggcga catggcaaat cgcgatcatc
     6121 tgattattgc ggatgccatt gtgtcgaaaa agaacacgcc gggaacgatg atgatcctgc
     6181 gggatgaaga agttccggcg ttgtttacca acaaaatctc tccgcatcag cttggcctgg
     6241 ccgacgtatt gtcggccctg cgcttcaccg gtgagtttcc gaaaaagctg accctggtcg
     6301 gcgtgatccc ggaatcgctg gagccacaca tcggcttaac gccgacggtt gaagcaatga
     6361 ttgaacctgc gcttgagcag gttctggctg cgctgcgcga atcaggcgtg gaagccatcc
     6421 cacgggaggc gattcatgac tgaagagata gcaggtttcc agacctcccc gaaggcgcaa
     6481 gtacaggcag cgtttgaaga aattgcccgg cgttcgatgt acgatctctc ttttctgcat
     6541 ccttcaatgc cggtgtatgt ttctgatttt acgctgttcg aaggtcagtg gacggggtgt
     6601 gtgatcaccc cgtggatgct gagtgcagtt atcttccccg gcccggatca actctggccg
     6661 ctgcgcaaag tgagtgaaaa aattggtctg caactgccgt atggcaccat gacctttacc
     6721 gttggcgaac tggacggtgt ttcgcaatac ctctcctgtt cgctgatgtc gccgctttcg
     6781 cacagcatgt cgattgaaga aggccaacgc ctgacggatg actgcgcacg aatgatcctt
     6841 tcgttgccag tcaccaatcc ggatgtacca cacgcagggc gtcgcgccct gctgtttggc
     6901 cgcaggaggg gcgaaaatgc atgagttgtc gctttgccag agcgccgttg aaattatcca
     6961 acggcaggcg gagcagcacg atgttaagcg cgtcaccgcc gtgtggctgg aaattggcgc
     7021 gctctcctgc gttgaggaga gcgccgtccg ttttagtttt gaaattgtct gccgcggaac
     7081 ggtggcgcaa gggtgcgatt tacatatcgt ctataaaccc gcccaggcct ggtgctggga
     7141 ttgcagccag gtggtggaga ttcatcagca cgatgcgcag tgtccgctct gtcacggcga
     7201 gcggttgcgt gtcgataccg gcgattcgct gatcgtcaaa agtattgaag ttgaataaga
     7261 ctgaggacaa cgcctaaaaa gcgcacggac ggtcccctcg cccctccggg gagagggtta
     7321 gggtgagagg gaacacccgc taaaggttct ttttcgcgtt tgtccggcaa tcgaaataac
     7381 cggagttaac aatgtgtatt ggcgttccag gccaggtgct ggctgtcggt gaagatattc
     7441 accagcttgc gcaggttgaa gtatgtggta tcaagcgcga cgtgaatatc gccctgattt
     7501 gcgaaggtaa ccctgccgat ctgctgggcc agtgggtgct ggtacacgtc ggatttgcca
     7561 tgagcatcat cgacgaagat gaagccaaag ccacattaga cgcactgcgc caaatggatt
     7621 acgacattac cagcgcgtga tgattagctt ttgtaggcct gataagacgc ggcagcgtcg
     7681 catcaggcat tgtgcacgat tgccggatgc ggcgtgaacg ccttatccgg cctggatggc
     7741 ttgctgcgac gaacaccaac ccttacccct gacgcttatc ttctgtattt gtctcaaaat
     7801 cactggcgtc atggcgctca tgcaactgct cgttcagcgg cccgttggtg cggttaacaa
     7861 tacgcccacg tttcactgcc ggacgttcgc ccacttcttt cgcccagcgt tgcacatgtt
     7921 tataacttcc cgcatccaga aactctgcgg catcatacac gccgccgagc accacgttgc
     7981 caaaccacgg ccaaatcgcc atatccgcaa tggtgtactc atcgcccgca acaaacttat
     8041 gctgcgccag ttgcttatcc agcacgtcga gcaggcgctt ggcttccatg gtaaagcggt
     8101 tgatggcgta ctcaattttc accggcgcgt aatggtaaaa gtgaccaaaa ccaccgccga
     8161 ggaacggcgc ggcaccctgt aaccagaaca gccagttcaa cgtttcagtc cgttttgcca
     8221 gatcctgcgg caggaagtag ccaaatttct ccgccagata aagcaggatc gaaccagatt
     8281 caaacacgcg gatcggcgga ttatgcgtat gatcgcgcag cgccgggatc ttcgagtttg
     8341 ggttcacttc gacaaagccg ctggagaatt gatcgccatc gccaatacga atcagccagg
     8401 cgtcgtactc tgcaccagta acgcccagcg ccagtagctc ctcaagcata atcgttactt
     8461 tctgaccgtt cggcgttccc agcgaataaa gttgcaatgg atgtttgcca acgggcagcg
     8521 ttttttcatg cgtcggacca gaaaacgggc gattgatatt ggcgaacgcg ccgccagcgg
     8581 atttatccca cgtccagact ttcgcgggct gataagtatt gtctgtcata gtgagttgcc
     8641 ttctgagtgg tagtgttgaa gcagtgtagc aggtcacttg ctgtacagaa tgtgaatttt
     8701 ttaacccttt gcgccagccg ctgcgatgag gtgtatgatt agtctgacct ctgtccggtt
     8761 aatgtcgctg agcaggtaat cagagcacaa caattacgcc aactgcttag gttgtattgt
     8821 taaaggtaaa gtgatgagca aaggaacgac cagccaggat gccccgttcg ggacattatt
     8881 gggctacgcc ccaggtgggg tagcaatcta ctcttcagat tacagttctc tcgatccgca
     8941 ggaatacgaa gatgacgccg tattccgtag ctatatcgac gacgaatata tgggccacaa
     9001 gtggcaatgc gttgaatttg ctcgccgttt tctctttctg aattacggtg tggtctttac
     9061 tgacgtgggt atggcgtggg agattttctc gctgcgcttc ctgcgagaag tggttaatga
     9121 caacatcctg ccattgcagg catttcctaa cggctcgccg cgtgcgccgg tcgcgggtgc
     9181 gcttcttatc tgggataaag gcggtgaatt taaagacact ggccatgtcg ccatcattac
     9241 ccaattgcat ggcaacaaag tccgtattgc ggaacagaac gtgattcatt ccccgttgcc
     9301 gcaagggcaa cagtggacgc gcgagctgga gatggtggtc gaaaacggct gctataccct
     9361 taaagacact tttgatgaca ccaccattct gggctggatg atccagacgg aagatactga
     9421 atacagctta ccgcagccgg aaattgcagg cgagctgctg aaaatcagtg gagcgcgtct
     9481 ggaaaacaaa ggccagtttg acggtaaatg gctggatgaa aaagatccgc tacaaaacgc
     9541 ctatgtgcag gccaacggtc aggtgatcaa tcaggatccg tatcattact acaccattac
     9601 cgagagtgcc gagcaggagc taattaaagc caccaacgag ctgcatctga tgtatcttca
     9661 cgcaaccgac aaggtgctga aagatgacaa cctgctggcg ctgttcgaca ttccgaaaat
     9721 cctctggcca cgtttgcgtc tctcctggca gcgtcgccgt caccatatga tcactggtcg
     9781 tatggatttc tgcatggatg agcgtggcct gaaggtttac gaatacaacg ccgactccgc
     9841 ctcctgtcat accgaagcgg gcttgatcct cgaacgttgg gcggagcagg gctataaagg
     9901 caacggcttc aatccggcgg aagggctgat taacgaactg gctggtgcct ggaaacacag
     9961 tcgtgcacgt ccgtttgtcc atatcatgca ggacaaagat atcgaggaaa actatcacgc
    10021 gcagtttatg gagcaggcgc tgcaccaggc gggctttgaa acgcgtatct tgcgcgggct
    10081 ggatgaactg ggctgggatg ctgccgggca actgattgat ggggaagggc gactggttaa
    10141 ctgcgtgtgg aaaacctggg cgtgggaaac cgcgtttgat cagattcgtg aagttagcga
    10201 ccgtgagttt gctgcggtgc caatccgtac cggtcatccg caaaacgaag tgcgtcttat
    10261 cgacgtattg ctgcgcccgg aagtgctggt ctttgagccg ctgtggacgg tgatccccgg
    10321 caacaaagcg attctgccga tcctctggtc gctgttcccg caccatcgtt acctgctgga
    10381 taccgatttc actgttaatg atgaattggt gaaaacaggt tacgcagtga aaccgatcgc
    10441 cggtcgctgt ggtagcaata tcgacctggt cagccatcat gaagaggtgc tggacaaaac
    10501 cagcggtaaa tttgccgagc agaaaaacat ctatcagcaa ctgtggtgtt tgccgaaagt
    10561 ggacggtaaa tacattcagg tatgtacctt caccgttggc ggcaactacg gtgggacgtg
    10621 tttgcgcggt gatgaatcac tggtcatcaa aaaagagagt gatattgaac cgttaattgt
    10681 ggtgaaaaag taatacatgt tgtagcaacc ccgtactgat tatgaagaat aattagtacg
    10741 gggattaatc aaaatattca atatttatat attccaatat ttattcattt catatgtgaa
    10801 tatattaacc agtggaatac ctgtgttttg attatttcta aaggttttga atgaatgctt
    10861 attgtctgat acacaagaaa taacactctt tttatcgtta aaaaatgata tttcactttg
    10921 cccatgccgt taaagtcact gataatgcgt ccgttcgtaa attcaaaatg gcgtaatcta
    10981 atatatgcta aatttatttg ttggccttga tatatatacg gggcttttgt tattacttgc
    11041 tctggcattt gtgttgttct acgaagcaat caatggtttt catgacacgg cgaatgcggt
    11101 ggcaaccgtt atttatactc gtgccatgca gccacaactt gctgtggtga tggcggcatt
    11161 ttttaacttt tttggcgtgt tattgggcgg acttagcgtt gcctatgcca ttgtccatat
    11221 gttaccaacc gatttgttgc tgaatatggg gtcaactcac ggtctggcga tggtcttttc
    11281 catgctgctg gcggcgatta tctggaacct gggaacgtgg ttctttggtt taccggcctc
    11341 cagttcgcac accttgattg gtgcgattat cggcatcggt ttaaccaacg cgctgttaac
    11401 cggctcatcg gtgatggatg cgttaaacct gcgtgaagta accaaaattt tctcctcgct
    11461 gattgtttcc cctatcgtcg gcctggtcat tgcgggtggt ctgattttcc tgctgcgcag
    11521 tttctggagt gggacgaaaa agcgtgaccg tattcaccgc attccggaag atcgcaaaaa
    11581 gaaaaagggc aaacgtaaac cgccattctg gacgcgtatt gcgctgattg tttccgctgc
    11641 gggcgtggcg ttttcgcacg gcgcgaacga cgggcagaaa gggatcggcc tggtaatgct
    11701 ggtactggtg gggattgccc ctgctggctt cgtcgtcaat atgaatgcgt ccggctatga
    11761 aattacccgt acccgcgatg ccgttaccaa cttcgaacac tacctgcaac agcatcctga
    11821 actgccgcag aagttgattg cgatggaacc tccattgcct gcagcatcga ctgatggcac
    11881 gcaagtaaca gagtttcact gtcatccggc aaataccttt gatgcgattg cgcgcgttaa
    11941 aacgatgctg ccaggcaata tggaaagtta cgagccgtta agcgtgagtc agcgcagcca
    12001 gctgcgccgc attatgctgt gcatctctga tacctccgcg aagctggcga aactaccagg
    12061 cgtcagtaaa gaagaccaga acctgctgaa aaaactgcgc agcgatatgt taagcaccat
    12121 tgagtacgct ccggtgtgga tcatcatggc ggtagcactg gcgctcggca ttggcaccat
    12181 gattggctgg cgtcgtgtag cgatgaccat cggtgagaag attggtaagc gcggcatgac
    12241 gtatgcgcaa ggcatggcgg cacaaatgac ggcggcagtg tctatcggcc ttgccagtta
    12301 tattgggatg cctgtctcca caacacacgt cctctcgtct gcggtagcag ggacgatggt
    12361 ggtggacggc ggtgggttac agcgtaaaac ggtaaccagc atcctgatgg cgtgggtgtt
    12421 tactttaccg gcggcaattt ttctttctgg tgggctgtac tggatagcat tgcagttgat
    12481 ttaaccttcc tgaaaatgcc cggtcctggc gatcgggcat ttccatttt
//
LOCUS       DADLSA010000112        12425 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.112, whole genome shotgun sequence.
ACCESSION   DADLSA010000112 DADLSA010000000
VERSION     DADLSA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.112"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(74..4534)
                     /gene="mukB"
                     /locus_tag="JN691_003044"
     CDS             complement(74..4534)
                     /gene="mukB"
                     /locus_tag="JN691_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAY7244430.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADRGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(4534..5238)
                     /gene="mukE"
                     /locus_tag="JN691_003045"
     CDS             complement(4534..5238)
                     /gene="mukE"
                     /locus_tag="JN691_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAY7244431.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5219..6541)
                     /gene="mukF"
                     /locus_tag="JN691_003046"
     CDS             complement(5219..6541)
                     /gene="mukF"
                     /locus_tag="JN691_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAY7244432.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6538..7323)
                     /gene="cmoM"
                     /locus_tag="JN691_003047"
     CDS             complement(6538..7323)
                     /gene="cmoM"
                     /locus_tag="JN691_003047"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAY7244433.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRLGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7459..8238
                     /gene="elyC"
                     /locus_tag="JN691_003048"
     CDS             7459..8238
                     /gene="elyC"
                     /locus_tag="JN691_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAY7244434.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8215..9108)
                     /locus_tag="JN691_003049"
     CDS             complement(8215..9108)
                     /locus_tag="JN691_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAY7244435.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9262..10008)
                     /gene="kdsB"
                     /locus_tag="JN691_003050"
     CDS             complement(9262..10008)
                     /gene="kdsB"
                     /locus_tag="JN691_003050"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAY7244436.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEIR"
     gene            complement(10005..10187)
                     /locus_tag="JN691_003051"
     CDS             complement(10005..10187)
                     /locus_tag="JN691_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAY7244437.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10239..11471)
                     /locus_tag="JN691_003052"
     CDS             complement(10239..11471)
                     /locus_tag="JN691_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAY7244438.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11508..>12425)
                     /gene="lpxK"
                     /locus_tag="JN691_003053"
     CDS             complement(11508..>12425)
                     /gene="lpxK"
                     /locus_tag="JN691_003053"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAY7244439.1"
                     /translation="GLVSGAIRLCYKLKLKRAWRAPVPVVVVGNLTAGGNGKTPVVVW
                     LVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTTTAQAGDEPVLIYQRTDAPVAVSP
                     VRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVEIVVIDGVRRFGNGWWLPAGPMRE
                     RAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNLRTGTRCDVAQLEHVVAMAGIGHP
                     PRFFATLKMCGVQPEKCVPLADHQSLNHADVSALVSAGQTLVMTEKDAVKCRAFAEEN
                     WWYLPVDAQLSGDEPAKLLTQLTSLASGN"
BASE COUNT         2865 a         3537 c         3085 g         2938 t
ORIGIN      
        1 ttatgtgcca tttttttagt gcagaaacgg agttttcgga aaaagaaaag gcggcattgc
       61 tgccgcctta attttaactc gcctgggaag gcgcttcgtc acttcctgga agcgtttcag
      121 ggagttgcgg cgcaaatcct cgcaggccga cgacgtgaac atgttcggta ttctggaaga
      181 ctttacgtac cagtttatag gtggtgcctt tttccggact gatattttcc ggcgctgcga
      241 tgatgagctg catttgcaaa cgttcacaca attcaaacag cgtggcgata gaacgagcat
      301 ccagtcgcgc tgcttcatcg aggaacagca ggcggcaagg agagatatct ttaccgcgca
      361 ggcggcgaga ttcatcttcc cagctttgta ccaccatcac cagaatcgac ataccggtac
      421 caatcgcctc accggtcgac aatgcaccag actctgcgcg caaccaacca tcggaaccac
      481 ggttaacctc aacttccatt tccagatagt tgcggtaatc cagcagttct tcgccaatgg
      541 tctgcggcgt gcgctgcccc atatcaatct gcggattaag acgctgatac aatttcgcca
      601 acgcttccga gaaggtcaga cggttgctgt taaacagatc ctgatgctgc tcgtgctgtt
      661 cagagagcac atccagcagc atggcgtgcg tttcacgcac gttcacgttg agacgcacgc
      721 tgttcacctg accaaacgat acgttctgca acccctggtt gagcatacgg atacggttct
      781 gctcgcgctg aatggttttg cgaatgatgt tcgccacgct gcgggaactg atcgccagtt
      841 tctgttcacg ggaggttaat tcttcggtca gacggctaag ttcaatctcc atctgttcga
      901 tagcttccac cggatcatcg gtacgaataa tatcctgacg aatacgttca cgcagatgct
      961 gataaaccgc cacgaagaac tgaattttac gctccggacg tttcggatct tccgacatgc
     1021 gcagcacgtc gcgcagatgt tcgttatccg ccaccgccag acgcagcgca cctaacgcct
     1081 tatccgacat ggaacgcaaa tcatcagcgg agagataagc cagctcacga cggtgtaagc
     1141 ggcgctcaac gccgttatct ttcaccatgc gcatcaccgc acaccagccc gctttggcgg
     1201 tcactacctg ctcgcgcatc tcaaagtaat cccgctccag cttgcgcagt ttgcgggtca
     1261 ggttgtccat ctccgcttcg cagaaggtaa gcgctttttc cagttgattg cggcgtgaac
     1321 ggttattgct cagttgcgca tgcagctcgt cacggcgaat acgcgcccgc tcttctgccc
     1381 ctctatcagc acgcacgccg atatcctgca attcacgttg cagatcgttg agtagctctt
     1441 ttttggtgtc gtaagaactt ttcagcgaag ccagcacctg gttgtactga ctcaactgcg
     1501 ctgcgtgacc gcgcaacgct tcgcgagcgc gggtacgctc cgcttccgcc tgttccagac
     1561 gttcacgcag tttttcgttg agatcgctgt taccgctaag catttctgcc gagtcagaat
     1621 agctaaagtg cgcacgacgc tgcaccactt ccgtcagggc aaacgcttgc tgacgggcat
     1681 cgcgctgcat ctgctgagag tacgcgtaat cttcttttaa ctgttcgaac tgttccgggt
     1741 cgctctgcaa taccgaaacg atcggttcca gtttcgccag ttgattgcca aactgctgaa
     1801 caaaacgcgc ggcttcctgg gcttcatcca gacgttcgcg gatttcatcg acgcgatccg
     1861 ccaggctgtc atcagccaac aggttgagac gcggcagaat gcggttcagc gccgtaacgc
     1921 cttctttcgc ctgctcaaac tgaatacgct gctgctggtt atcattttca tgattactca
     1981 acgcccgctc cagttcgact cgacggctgt tcagttgacg gatttctgct tccgggtcag
     2041 actcaaacgc caccgccaga tgactgccga taaagcggct gaacgcctga tgcagacgct
     2101 gggttttctg tacatcaaag gagagcgtgg cgaagcgttc ggaaagcact tcacgctcgg
     2161 catggaggct ttcaatacgg ctttcacgcg cagcacgacc aaacagcggc acttccggga
     2221 aacgtgaata acgccactgt cgatcggcga ttttcaccac caccgctttt tccagctcat
     2281 caacgctgaa cacgctatca tcgaatgact gcggatcccc ttcgatcaaa tagagatctt
     2341 ccgggcaatc ggtcaagcct tccaggtgtt cggttacctg tgacagatct ggcaccacga
     2401 tggcgtggcg tgacgggcca tacagagccg agaagtacgg cgcatcttcc aggctaacgt
     2461 cgtcataaat ttctgacagc agcacaccac caaaacgctc cgccagcgcg ttcagacgct
     2521 gatcttcaga gccgccaggc tggcttaaac gttcgatctc ttcatcgacg gcgtttttgc
     2581 gcgcgcctac ttcatcgcgt tcaacaatcg cctcgcgctc acgctccagc aactgttgca
     2641 gatattcagt gacgtcctgg ctggaggaga actcttcgcc gcactgttca ctcaactggt
     2701 tgagactgtt ttgcgctgcc agccaaaccg gtgcacgctg catcaaactc tgaatgcgag
     2761 actgcagctg ttcctgttcc tgccgcagtg ccatccgctc ttcacgggcg ttagatacgc
     2821 tatcggaaag agaggcaatg cgtgcttcca gctcctgatg cagagcttcc agttcatcaa
     2881 tatcaaaatt cttgccctga cgtttgcaga aatctgccag cagacgctca gcttcttgct
     2941 gctcacgcag acgctgttcc agttcgctta agcgcatccg caacggctga acctgctctg
     3001 ccaggtggcg ctgatcaacc ccttcgcgca atagttcgcg agcgacatcc cacgcctcgt
     3061 tacgcgccag cgggccgttg attgccacca ccagctgata agcctgctca aactggctgt
     3121 gcgcggtttg cgccatgctc attttctgct caagagagag cattttttca gtcgcttcca
     3181 gctctttcgc ctggaaggtt tccagccatt cggcggcgct gtcggcggtt aagtccggca
     3241 gatggcacag ttctttggca cgattaagcg cagcaatcgc ctggttatac tggatcgcgc
     3301 gcgtttgctg gacgtccagc gcctgctggt agtcagcaag ctggcttttc agctcgtcca
     3361 cttccagttc ggcagcttcc gcgcgagcct cattctcttc ctggcgttcg atggcttctg
     3421 ccaccacttc attttgctct tccagacgga tctgcagctc atcgagatcc acttcgtagc
     3481 gttcgatctt ctcctgctga cgcaatgcgg tttgcaccag gttcaggtga tcgctggccg
     3541 cctgataatc cgcttccaga tcgccttcgg caccgttgtg ctctgccagc tcacgcgcca
     3601 tatcgacgtg tttgtactgc tcagccgcca gttgctgacg cgaagtatgt agctcgcgac
     3661 gaaactccag ggctttgtcg agatggacac ggcgctcgtt ggcgtgacgc atgtagtccg
     3721 ccgccacgta gttggtagct tcgctgatca gatgcttaaa caggtcacgg tccgactggg
     3781 tgacacgaat cgcttccagc gtcatgcggt tttcacgcag cgccgcttcc atgtcctgga
     3841 acgctttacg cacgccgctg ttttccggca acaggtagtc gcgcagagaa cgggtaatgg
     3901 cactggagat cccgccatac agcgaagctt cgatcagacg atagaatttg ctacggtcag
     3961 atgcggagcg cagacgacgc gcgatgatgc ccagatcgaa catcagcgag tggtaatcgg
     4021 taatggagtt gaactgttta aactgcacgc cttccatcgc ctcgagcttg tctttcagct
     4081 cgttaagcgg cagcacgcgc gcctggcgtt cgttcagggt ttcggtcacc agctgtgtcg
     4141 gctgcaccga catcggcagt ccctgaatgg caaatggttt gatatccact ttacgatcgc
     4201 gtccggcaac ctgttgcaga cgcacaccga ccaccacgcg ctggtggcgc gagttaatgg
     4261 tgtcgagcat cgaataacag acacccgctt tcagcttacc gtgcagacct ttatcgcgcg
     4321 aaccgctggt ggccccggct tccgtagtgt tacggaaatg cagcagggtc aggtcgggga
     4381 tcagcgccgt aacgaacgcc gccatcgtgg tggatttacc cgccccgtta ccgccggaaa
     4441 gcgtcgtgac cagctcgtca aggtcaaaag ttcgggcaaa aaagccgttc cagttaatca
     4501 gcgtcagtga gcgaaattta ccgcgttcaa tcattattct tcctctccgc tatctggctg
     4561 actctcttcg gtttcatcgt tgagttgcag atgattttca atcggcattg cttcgccatc
     4621 acgaatcagg cgacgctgtg cttcacgggg atcgtcgcca gcacgcacat cggctccgaa
     4681 gcggaacacc gattcggtaa tgcggaactt gctgctgtcg tggcccataa accacaccat
     4741 gcctaaacga cgcagacggt tgagcgaaga acgtactttc tcctgcaact tctgacggtc
     4801 aacgtctgaa ccggttgaac ggttgttcac cagtttcagc agtttcgctt catcggccag
     4861 ggtgagcagt tcgtcgtaca gttcctgctg ggtgaaaatc ccctcattcg ccagccgttc
     4921 cgggctgaga tagagataac agaggatttt cccgaccatc atatccagtt ccgacaagac
     4981 ggaacgcggg ataagagtgg tggaacgtgg gcgtaaatag aagaaccctt ccggtgcgcg
     5041 aataagctca acgttataac gcgcgtaaaa ctcttccaga tattcctgaa aatccatcag
     5101 gaatgcatga ttatccagtt cgtcgaggcc aatatggcgg cctgaacgta aggcgctgtc
     5161 cagcgccgga aataacggat tcgccagcgc ctgcgccagc ttaaccggca tcacttgttc
     5221 aatatttgtc gatgacatgc gcctgtacct tggctccgta atcattaatc ggctgccatt
     5281 tcgctggcag tccggtgaaa tctgcttgcg ctacgccaag acgtaccgcc tgatcaataa
     5341 caatacgcgc aacgtcaaag tgacgtgcac gcggatactg tgacagatat tcgcgtacca
     5401 ccagaccaag atccagcggc acttgtctgg ttttgtacac ggcaagttgt tcttcgatga
     5461 tcgccgccag ctgttcgcgg atctcgttaa actcttcgta ttccagatcc tcaggaagtt
     5521 ccccagtcac ttcttcatcg cgcagtgcca tctcttcgtc acgcatatcc agcagacgat
     5581 cggcattggc ataagttagc gcccacggct catcaaaata ggtttgtacc gactgacgta
     5641 accgctgagc aaagacgcgg tttttatcca tatcgatcgc ggtacgaata aatttgtgta
     5701 cgtggcggtc gtagccaatc cacaagtcga tggattgctg gccccaactg ataatacgat
     5761 cgagtttgct ctgcagatcg aacaccagac gatcgacgaa atgcagatcg tcatgggtca
     5821 tcgtcgcatc ctgaatgcgc aacagattag cctgcaattt gtcgcctgcc gcttccagcg
     5881 tatcctgcaa ttcacgcagc gttccggaag tttccgaaag caacaattca cagctggaaa
     5941 tcgccgcccg ccagtctttg ttcagcaact gggcgatatc gtccttcacc tgctgctgct
     6001 gttcgtccat cagacgttgc gtcaggtcga tactgtcgaa aatttctgct accgaatatt
     6061 tcagtggcgc atagacatta cggtgccagt gaaattcatc accgccctct tcggcggcat
     6121 ctgctgcgcg tttgagctca cccgccacaa tcgacaactg catagaaaga cgcagcgtag
     6181 aaaactcgcg ctgacggatg tagtagtcag taatgccgat gccgagcggc gtcagacggt
     6241 aaattgcgtt cccttccgcc tgctcgctgg taaagcggtt cagcagacgt tgacgcacca
     6301 tgtcgttgat cgcgttattg gcgcgcacgc cgatggtttc gctggtttgc tcaaacgcat
     6361 cactcacatg gcggaatgca tccaccagct cgccttcact catctcacca tccagacgct
     6421 cgccgttcag cgtggcaacc gccagcagaa aagagagtcg gtctaccggc agcgagatgg
     6481 agaagtcatt ttttctggcc caggcaacca gttcggggac tgtctgggaa aattcactca
     6541 tactttatcc ttgctctgcg gtttgcgcgc ggtgacatga atataacgcc ctagggtaat
     6601 atacggttcc tgacggcaat aacgcgtttc taattcaagt aatgcttcat agcagtcgcg
     6661 ctgctggtgc ttctcgcgca gataatcatg aaacacgcga acgcctgtct tacccataat
     6721 ttgccaacca gcttcttcca gccacagata aacctgcgcc gggtcgcgtg gataatctgg
     6781 cgaaagcgtc cgttttttct ttttcggcat tcccgcctgc acgtaatcaa aattcccggc
     6841 gaccatgtta tgcatcaaca aaccatgcgc attgtagaac attaacgaca acactccgcc
     6901 tagacgcaac actgaccaca gggtctgcaa tacactgcgg ggatcggcca cccactcgag
     6961 caccgcatgg aacaatatca gatcaacggg cgtttccaaa tgcgaagcaa catcctgagc
     7021 ggcgcaatgt ataaattgca tgttgtcgct cacacctttt gcttctgccg cctgttttgc
     7081 gcggtcgatc atctgcgcag aaagatcgca taaaataacc tgatgcccgc gctcggccat
     7141 tttgattgcg gtctgccctt ctccgccgcc agcatccagc acacgcagtt tttgcgggcc
     7201 catttccgcc agcacgcgat cgagatcctg ccacagaata gcctgtcgaa gctgcccttt
     7261 ggtggtgccg taaatgttac gggaaaactt ttccgcaata tcatcaaaat tgcgatcctg
     7321 cattgctttc tccaccggtt ggctaagacg ttttctgata aagacgctat tttgtcacag
     7381 ccacggcgag aatgaacccg tctggtgtaa tatcgctggc gatcccttgc tatatggtta
     7441 aaaaaggaac cagaaaggat gctttttaca ctgaaaaaag tgattggcaa tatgttgctg
     7501 ccacttccgc tgatgttgtt aattattggc gctggcctgg cgctgctgtg gtttagccgt
     7561 tttcaaaaaa ccgggaagat ttttattagc attgggtggc tggcactctt gttattgagt
     7621 ttgcaaccgg tagccgaccg cctgctgcgt cccatcgaaa gcacctatcc gacatggaat
     7681 aattcgcaaa aagtggacta tatcgtggtg ctcggaggtg gttatacctg gaacccgcag
     7741 tgggcaccga gctctaattt aatcaataac agtctgccac gcctgaatga gggtatccgt
     7801 ttatggcgcg aaaatccggg ttcaaaactg atcttcacgg gaggcgtagc aaaaaccaat
     7861 acggtgagta cagcggaagt aggtgccaga gttgcgcaat cgctgggcgt accgcgcgag
     7921 caaattatca ccctggattt gccaaaagat accgaagaag aagctgcggc agtgaaacag
     7981 gcgattggtg acgccccttt cctgctggtg acctccgcat cacatctgcc gcgggcgatg
     8041 atcttttttc agcaggaagg gttaaatccg ctaccagccc cggcgaatca gttggccatc
     8101 gactcgccgc ttaatccgtg ggaacgggca atcccttctc cggtctggct gatgcatagc
     8161 gatcgtgttg gctatgaaac gcttggccgc atctggcagt ggctgaaagg tccgtcaggc
     8221 gagccacggc aagagtgatt ttgatgccag atcgaagttg cgccgactaa atcgtccggt
     8281 attaaccagt tgcgcaactt catcccataa cacatacaac caacgccgcc agatgaacga
     8341 ctccgccact ggcgcgcgtt gcaggtaact ccagagcaaa tcttccgcca gagaattatc
     8401 cattagtcgg aacagttcgt attctcgcgg tgcccaaagc attagtcccg gcccgaccat
     8461 cgccagtaac tgatcgctgc gcgaatcttt cagcatgctg cgtaaacaga agttaccgtg
     8521 aatcaacaca caattgtcgt taaagccttc aaataatgcc gggagacatt cgcgagtgcg
     8581 aaacaggatc cgcttatcct gcatcgtcag accggtgtta ttgaactgat tgagcgtggt
     8641 ccatagcact tcaacatgtt gccggtacca tgagggccag aaattttcct gagtattgtc
     8701 gaccgcgccg acgcaaccgc gactgtcctg acggtgccag gccagtaagg cttcaacgat
     8761 ttggtctttg agctgttccc agcgttctgg tgttcgggct ggtgcctcca ccgaaacacc
     8821 acgcatccgc tccagtagca ggacatcagg gccggggtgt tcttcatgcg tcatcacacc
     8881 ataaatagtc ggcatacgga cagtcccact tcttgccagc atggtggttt tccatgccag
     8941 ttgtcgggct tttccgggcg tactaaagct tcttgccatt aacggcattg ggtttccctg
     9001 gctgtcatac aaagcccaca acgccgtatc cgctttttca ttgacgcact caatacgact
     9061 gagtttttcg cccagtaaat ggcttaattc ggcacgcagc tgttccattc agattaccct
     9121 cataaaagct actgctttca taatgagcgc caatataaaa gatgtcacct gaatgagatc
     9181 aattgttgaa aagaaaaatt gaggaaaatc gtaacggtac gacactcctc ccaaaattgg
     9241 ctgaagtgtc gtgaagtgaa attagcgtat ttcagcgcga acgcgctcaa gatcttcagg
     9301 ggtatccaca cctgtgccag gaacttcctg agcaacagca acatggattt tttcgccgta
     9361 ccacagaaca cgaagctgct ctaacatttc gatgtgttct aacggacttg cctgccagtt
     9421 gacgtaacga cggataaagc ctgcacggta gccataaata ccaagatgac gcaggaagtt
     9481 atcgccaacg gtttcaaggc cttctgcaaa acgatcacga tcccaaggaa tggtggcacg
     9541 tgagaagtac agtgcatacc cttcagcgtc gagaaccact ttcaccgcat tcgggttaaa
     9601 cgcttcttcc gcattgtgga ttggcaccgc cagagtcgcc atacccacct gacgctgagc
     9661 gaggttatca gcaacctgac gaatgattgt cgcagggatc atcggttcat caccctgcac
     9721 attaacgatc accgtgtcgt cgctgaatgc gcatttttcg acaacttccg ccagacgttc
     9781 tgttcctgac tgatgatcgg cgcgcgtcat acatacttca ccgccagcgg cttcaacggc
     9841 gcgggcaaca tcctcatgat cggttgccac gatgatgcgc tcggcacctg attcacgcgc
     9901 gcgttcaaga acatgaacaa tcatgggttt gccgttaata tcaaccaatg gtttaccggg
     9961 cagacgcgtg gacgcgtagc gcgcgggaat aatgaccaca aaactcatga tttactctca
    10021 tcagcagtca gcacgcgggc ttccgtttcc agcaacactg gaatgccatc gcggagcggg
    10081 aaagccaggt tatccagttt gcaaataagc tcttgttttt cctggttata ccaaagtttt
    10141 ccgttgcaaa ccgggcaggc aatgatttca agcaaacgat gatccatagt tcctccagat
    10201 ggatcgggtt atgaatgcat aaatcttatc ataatcattt atgcgacggg gtctatttcc
    10261 cagccagtgc ggcaatcagt aaagagacct tgcgggcagc gtcctaatgt cacccgcgtt
    10321 gcctgctgcc agctcgcgaa atcagtaatc gcctgacgta accctttttg cagcattgtc
    10381 gttggtttaa tgccttcctg taaccacaga gagataactt caaggatgcc tgtctggcga
    10441 tgcattttgg catccattcg cccaactaat tgcccacgat gtaataatgg cagaacaaaa
    10501 tagccatact ggcgtttcgg cgctggggta tagcactcca gccggtagct aaaatcaaaa
    10561 agctgctctg cgcgtttgcg atcccagaca acaggatcaa aaggcgaaag taccgcgctg
    10621 tgcgttgcag tgagctttcc ggccagcgca cgctcgagta gcggcagcaa atcagcatgc
    10681 agccaaagat tgcccaattt ttcaacatgc acagcaatga tttgctgctg ttcagccctc
    10741 gcttcgcgcc acgccgccag tgccggacgt ttcagccgat agtaatctgc cagccactgt
    10801 tcgcggaata ttcccaggct acgcgcactg ttatccagca tgatgatttc tgcttctgtt
    10861 tgcgaaacga gatcgcgctc atcatcccag tcaggcatga cacggtgggt taaatcataa
    10921 acgcgctgga agttgcgccg ttcaatcacc atcacctttc cggcagtaaa taaaccttcc
    10981 agatgccgtt tatgcggctt ccattcccac cagccgcttg cacctttacg aggatgctca
    11041 aaatcggctg aacgtaccgg ccctttatca tgaatatgct gaattaactg tgcaatttcc
    11101 gcctcatgtt cctgcatcca ggcgtctttg tatttccagc ccattttttc aggtgccagc
    11161 atgcggtggc gaataagacg aaagtcgcta cgcggcataa agcaggcttc atgcgcccag
    11221 tattccatta attcgccacg cgccagagac tcatccagcc actgggcagg ataatttccc
    11281 agacgactga aaagcaccag atatggacta cgggcaacaa tattgatggt atcgatttgc
    11341 agcaaggaca tgcgggagat cgttgccgga atatcctcca acgacgctcg acggcggggt
    11401 ttgtttaaca ggccttgtgc ggcaaggtga agattacgcg catcagcaag ggagaggtgc
    11461 ggcagcgaca ttcatgactc catcaatcga acgctgccgc ggcgtaacta gttgccagaa
    11521 gccagcgagg ttagttgcgt aagcagtttc gctggttcat cacctgaaag ctgtgcgtct
    11581 acaggcaaat accaccaatt ttcttctgca aaggcccggc atttcaccgc atctttttca
    11641 gtcattacca gcgtttgccc ggcgcttacc aacgcactga catccgcatg gttcaaagac
    11701 tgatgatcgg ccagcggtac acatttttcc ggttgtacgc cacacatctt cagcgtggca
    11761 aaaaagcgcg gcggatgccc aatccccgcc atcgccacta catgttcaag ctgagcaacg
    11821 tcacaacgcg taccggtacg taaattcacc gcctgacccg gcagcagatg catggggatt
    11881 tcaccgctgc gagggacacc gccgttgacg attaccgcat caaccgactt taagcgcccc
    11941 gctcgctcac gcattggccc cgccggcaac caccagccat tgccaaagcg acgcacacca
    12001 tcaataacga caatttccac atcacgcgcc agacggtaat gctgtaaacc gtcgtcggtt
    12061 acgatgatct gcacatcagg gtgttgcgcc agaatggctt ttaccgcatc agaacgaacg
    12121 ggagaaaccg caacaggcgc atcagtgcgt tgataaatca acacaggttc atcacccgcc
    12181 tgtgctgtgg tggtatctgc cgacaataac agcggataag attcagcctt accaccatat
    12241 ccccgcgata cgacccccac gcgaataccg cgctgttgca actgttccac cagccagaca
    12301 acgaccgggg tttttccgtt gccgcctgcg gtgagattac caaccacgac aaccggtacg
    12361 ggggcacgcc aggcgcgctt cagttttagt ttatagcaaa gacggatcgc gccactcacc
    12421 aggcc
//
LOCUS       DADLSA010000113        11958 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.113, whole genome shotgun sequence.
ACCESSION   DADLSA010000113 DADLSA010000000
VERSION     DADLSA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.113"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..163
                     /locus_tag="JN691_003054"
     CDS             <1..163
                     /locus_tag="JN691_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7244440.1"
                     /translation="LTWRGMYQQLLKVDPLKCELCGGQMRFTGLKMGYRLAEQIMMHE
                     PLARMRWCG"
     gene            312..597
                     /locus_tag="JN691_003055"
                     /pseudo
     CDS             312..597
                     /locus_tag="JN691_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            999..1535
                     /locus_tag="JN691_003056"
     CDS             999..1535
                     /locus_tag="JN691_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7244441.1"
                     /translation="MKYIVFVSALLISNTGKGGGQRVVFNVDIVGSACHVVVDADGFG
                     NSGRLIFGTYRKSSGVPVLPRDFVVRLYESGATVQGCSAFRAGQVATLDFGNPGQLDA
                     KGVVTRGAGDGIRVDVRAVDTQADYRGRLTQDSHSVNYPVEFAVKGQFRFRAQPVFPA
                     NVKAGEYTGALTFVVTYQ"
     gene            1550..3988
                     /gene="faeD"
                     /locus_tag="JN691_003057"
     CDS             1550..3988
                     /gene="faeD"
                     /locus_tag="JN691_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbrial usher FaeD"
                     /protein_id="HAY7244442.1"
                     /translation="MCAGKKVVAELVSSLFSRRAALAVSAAFASTTGVAGEKLDMSFI
                     RGGAGVNPEVWAALNGSYAPGRYLVDLSLNGKEAGKQILDVTPQDSDELCLTEAWLAK
                     AGIYVSADYFREGYDATRQCYVLTKAPSVKVDFDVSTQSLALSIPQKGLVKMPENVDW
                     DYGTGAFRVNYNANANIGRNNTSTFGSADLKANIGHWVVNSSASASGGDSGDNSATIN
                     AFTATRAIRALSADLTVGKTSTGSSLLGSTGTYGVSLSRNNSMKPGNLGYTPVFSGIA
                     NGASRVTLTQNGRLLHSEMVPAGPFSITDVPLYTSGDVTMKITGEDGREQTQLFPLSV
                     MAGQLNPGQHEFSVAVGLSDDDSDLKGGVFAASYGYGLDGLTLRTGGVFNRDWQGISA
                     GAVLGLSYLGAVSADGAYAMAEYRDGSRSGNKVQLSWSKQLEMTNTGLRVSWSRQSEE
                     YEDMSSFDPTELWSQSNHGRRPKDEWNAGISQSVGGLFSLSVSGWQRSYYPAQMTGRY
                     RYSDDNGLDTGITGTLSTQIKGVSLNLGWSGSRNSQGENNRSASASISVPFTLFERRY
                     SSSTSVSTSKGGGTGFSTGVSGSLNDRFSYSLGGGRDSGGGVSSYLNASYSGDRAYLN
                     GALNHSQSGGTSGSVSVSGSVLVVPMAKDIMFSRTTGDTVAVVNVKDTPGVKVTSGDG
                     QTDSDGNLVVPLNSYDWNTVTIDAGTLPLSTELTNTSQKVVPTDKAVVWMPFDALKVK
                     RYLLQVKQRDGEFVPGGTWARDSKNTPLGFVANNGVLMINTVDAPGDITLGQCRIPAA
                     RLQDTEKLQEITCE"
     gene            3981..4763
                     /gene="faeE"
                     /locus_tag="JN691_003058"
     CDS             3981..4763
                     /gene="faeE"
                     /locus_tag="JN691_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbrial chaperone FaeE"
                     /protein_id="HAY7244443.1"
                     /translation="MSKRNAVTTFFTNQVTKALGMTLALMMSCQSAVASLAADQTRYI
                     FRGDKDALTITVTNNDKERTFGGQAWVDNIVEKDTRPTFVVTPSFFKVKPNGQQTLRI
                     IMASDHLPKDKESVYWLNLQDIPPALEGSGIAVALRTKLKLFYRPKALLEGRKGAEEG
                     ISLQSRPDGRTMLVNTTPYIFAIGSLLDGNGKKIAADNETAQKLLMFMPGDEVQVKGN
                     VVKVDSLNDYGELQTWTINQKKSAISSAQKSSDTEGNSSDKK"
     gene            4798..5289
                     /gene="faeF"
                     /locus_tag="JN691_003059"
     CDS             4798..5289
                     /gene="faeF"
                     /locus_tag="JN691_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeF"
                     /protein_id="HAY7244444.1"
                     /translation="MKKTMMVVALALSALSIQSTLAAEYSEKAQYLGVVNGQVVGNSV
                     VKVTRIPTDPVLYRSGDTTPLPDRLTIRNAESRAASGGLAYITVKQVLPDNGEARITL
                     KTALMVDGKKVAISARQQGEDMVITLPEAQKQIELRTDAPAELEVPVSYRGNLQIALQ
                     VED"
     gene            5527..6327
                     /locus_tag="JN691_003060"
     CDS             5527..6327
                     /locus_tag="JN691_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cshE pilin"
                     /protein_id="HAY7244445.1"
                     /translation="MKKTLIALTVAASAAVSGSAMAWNPSGLGGNGGTFSMSGTLSKA
                     EKTIPWAVEIGAPVTALDVTVKPDQKNVFILLEKDIPILGIRSNPTGFMGEKGIAPQI
                     DYNKAADVDYMDKGNLPAQVNVTSSDGKKIGTLAFVMRVACQASEKRGEGDDKLESNM
                     LFASSKGKAFFGGVAKDDKGVWSDAAAYTWATHLFSSIANSWDTKIPYTAGDTDETDF
                     SNGDRLYRGYYASGLAKDIPMELVLDQTITSATPVPWKASLPIVISYM"
     gene            6548..7345
                     /gene="faeH"
                     /locus_tag="JN691_003061"
     CDS             6548..7345
                     /gene="faeH"
                     /locus_tag="JN691_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeH"
                     /protein_id="HAY7244446.1"
                     /translation="MKITHHYKFFLSAVILVTIFYSAVPYANILDGGEIQFNGYVTDE
                     APKWAWQISSPDQTWSVDTADARTENGQLVFNLHDKGALPFLEGHLHDVAERGGPGFT
                     PFITFSSNGQPFTVTEGNGASAQHFRASVPVRDPETGNVSGQLFFTLNQGMAVSTGRQ
                     DDGASVPAGMSLVSGQSVTDVQPGSLPQGLKARLSSLLLMNQNFGNGMNAVDNGQVIN
                     QGILTDGRVMDLAAAYASEVSDFELRLPAEGTPAAWQAGLNVTVTVQ"
     gene            7375..8127
                     /gene="faeI"
                     /locus_tag="JN691_003062"
     CDS             7375..8127
                     /gene="faeI"
                     /locus_tag="JN691_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeI"
                     /protein_id="HAY7244447.1"
                     /translation="MKKVTLFLFAASHLPSCVLAWNTPGENFSGELKLEGPVTSTRNP
                     WVWKVGQGNGGLEVKQSGGGGGIQVALPAMTVLLGKTTLTTPAGREGLSPRVSYGKGT
                     EGFSLVWTAPGMAEVTLPVTGDNNARAGTFVFRIQAVGVLRHVQGGQPVYVGMYDDLN
                     TNGLPGESTAMKVSDIPGVLQKMFSGEGPGWLQTMTLSGYSGVSHFSDDFLRQVEGVY
                     GAQTVAGSGELRLNGEMPEHWRVSLSVNIEYQ"
     gene            8452..9678
                     /locus_tag="JN691_003063"
     CDS             8452..9678
                     /locus_tag="JN691_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY7244448.1"
                     /translation="MSGTIPKIKTGENVLSAAIQRIEWLFGTFPSVCLSFSGGKDSTV
                     LFHLVSEVARKKRRRFSVLFIDWEAQYQCTITHIQKMQKMYLDVTDRFYWVALPLTTV
                     NGVSQFQPEWICWEPGVTWVRHPPEYAITDMAYFPFYRYAMTFEEFIPAFSSWFARDK
                     YLVAVLTGVRADESLNRFMGLVSRRKQRYADDKPWTTASPEGFYYTMYPLYDWKARDI
                     WIYNARTRAIYNPLYDLMHRACVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMCE
                     RVSGAASGALYANESGAYFALRKRITKPPHHTWRSYAMFLLDVMPERTAEHYRNKIAV
                     YLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRHYE
                     RYLQRMKERRKEWGIL"
     gene            9663..10301
                     /locus_tag="JN691_003064"
     CDS             9663..10301
                     /locus_tag="JN691_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAY7244449.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            10529..10921
                     /locus_tag="JN691_003065"
                     /pseudo
     CDS             10529..10921
                     /locus_tag="JN691_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10940..11413
                     /locus_tag="JN691_003066"
     CDS             10940..11413
                     /locus_tag="JN691_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7244450.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
     gene            11565..11827
                     /locus_tag="JN691_003067"
                     /pseudo
     CDS             11565..11827
                     /locus_tag="JN691_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904911.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         3033 a         2527 c         3377 g         3021 t
ORIGIN      
        1 actcacatgg agagggatgt atcagcagtt actgaaggtt gacccgctga agtgcgagct
       61 gtgcggaggt cagatgcggt ttacgggact gaagatgggt taccgtctgg cagagcagat
      121 tatgatgcat gagccgctgg cacgaatgcg gtggtgcggc tgagcgccgc agaggggaag
      181 ttgcgtccat gttgagggga acggagcaaa aaaccatcag agatatattg aatcaatcaa
      241 tgtaagcaat atcaagtgtc tataccaatt acgtgatgat caggtcgtgt tgaataaacg
      301 cgcattcttc ggtgcttttc taatcttttg gggatttaag ttcctgtcga tgaactgctg
      361 gcgctgcttc ccccttttac catcggtatg ttgaccactg acgaatgggg tagttacacc
      421 agagagttac cgaaagagaa gtatctgact ggtaagattt ttactcagcg cattgagcgc
      481 aacaatctgc ccctgagaac ccgaatcaaa cgcctggctc gcagaaccat ctgtttctct
      541 cgctctgtaa aatttcatga aaaagttatc ggtgctttta tcgaaaaaca tatgttcctg
      601 gagtcatcac ccttttcctc ttggtgttat cccatcaata tttcggccac gtggtttacg
      661 tttattattg ataataatca atgaactgcc gagtaaaaat aactgctgat atacgggatc
      721 ccatgtcaac tcccgcatta tatattatac ctcaatagcg ttacattatt aatgtatgta
      781 aatgttaatt aaccaaagca ttaatcaatt gttatataat tgcgtcgcag gtaaacgcca
      841 aatcaacgca aatacaaata gtaaaaatgg ttcatgtttt ggcactattt ggtttttttg
      901 tttttaacgg gtggatattt gtttattttt tttctaaatt atttttatgt aattttcata
      961 aaaatggggt gtgagttatt aactctaaat tagtgagtat taaatatatt gtgtttgtct
     1021 ctgctttatt aatttccaat acgggaaaag ggggggggca gcgagttgtt ttcaatgtgg
     1081 atattgtagg ttctgcctgt catgtggtgg tggatgcgga cggtttcggc aacagtggtc
     1141 gactgatatt cgggacttac cgtaaatcca gtggcgtgcc tgtattgcca cgtgactttg
     1201 ttgtgcgctt gtatgagtcc ggcgctacgg ttcaaggttg ttcagccttt cgagcggggc
     1261 aggtcgcaac tctggatttt ggtaacccgg gccaactgga tgcaaagggc gtggtcactc
     1321 gcggtgctgg tgatggtatt cgcgtggatg tacgggcggt ggatacacag gcggattatc
     1381 gcggacgtct gactcaggac agtcattcgg tgaattaccc ggtggagttt gctgttaagg
     1441 gacagttccg ttttcgtgca cagccggtgt tcccggcgaa cgtgaaggca ggggaataca
     1501 caggggcgct gacctttgtg gtcacgtatc agtaacagag gaaaggtgtg tgtgtgcagg
     1561 aaagaaggtg gttgcagagc tggtttcgtc tttgttttcc cgtcgggctg cgcttgctgt
     1621 atcagctgct ttcgccagta ccacaggtgt tgcaggtgaa aaactggata tgtcctttat
     1681 ccgggggggt gccggggtta atccggaagt ctgggcagcg ctgaacggca gctatgcgcc
     1741 gggacgttat ctggttgacc tgtccctgaa cgggaaggag gccgggaaac agatactgga
     1801 tgtgacacca caggacagtg atgaactgtg cctgacagag gcatggctgg cgaaggccgg
     1861 gatttacgtc agtgcagatt actttcgtga gggatatgac gccacacggc agtgttatgt
     1921 gctgacaaaa gcgccgtcag tgaaggtgga ttttgatgtt tccacccaga gtctggcgct
     1981 gtccattccc caaaaggggc tggtgaagat gccggagaac gtggactggg attacgggac
     2041 tggtgcattt cgcgtgaact ataacgcgaa cgccaacatc ggccggaata acacctcgac
     2101 ctttggctcg gcagacctga aggccaatat cgggcactgg gtggtgaatt cttctgcctc
     2161 agccagcggt ggtgacagcg gggataactc cgccacgata aacgcgttca cggccacccg
     2221 ggccatccgc gcactgagtg cggacctgac ggtcgggaaa acatccaccg ggagcagtct
     2281 gctgggcagt acgggaacgt acggagtgtc gctgagccgg aacaacagca tgaagccggg
     2341 caatctgggg tataccccgg tgttcagcgg cattgcgaac ggggcgtcga gggtgacact
     2401 gacacagaac gggcggttgc tgcattcgga gatggtgccg gcaggtccgt tctccatcac
     2461 ggatgtgccg ctgtacacca gtggggatgt gaccatgaaa atcaccggcg aggacggtcg
     2521 tgagcagact cagctcttcc cgttgtcggt gatggccggg cagttaaacc cgggacaaca
     2581 cgagttcagc gtggcagtcg gtttgtctga cgatgacagt gacctgaaag gcggcgtgtt
     2641 tgcggcgtca tacggttacg gtctggatgg actgacgctg cgcaccgggg gcgtgtttaa
     2701 ccgggactgg cagggtatta gcgccggggc tgttctgggg ctgagttatc tgggggcagt
     2761 gtctgctgac ggggcttatg ccatggcgga ataccgtgac ggcagccgca gcggaaataa
     2821 ggtgcagttg tcctggagta aacaactgga gatgacgaac accggactgc gggtgagctg
     2881 gtcacggcag agtgaggaat atgaggacat gtcctccttt gacccgacag agctgtggtc
     2941 gcagtcaaat catggtcgcc ggccgaagga tgaatggaat gccggtatca gtcagtcggt
     3001 gggggggctg ttcagtctgt cggtgtccgg ctggcagcgg agttattacc cggcacagat
     3061 gactggccgt tatcggtaca gcgatgacaa tggtctggac acagggatta ccggtaccct
     3121 aagcacacag attaagggcg tcagtctgaa tctgggctgg tccggctcac ggaattcgca
     3181 gggagagaat aacaggtcgg cgtcagcctc tatctcggtg ccattcacgc tgtttgagcg
     3241 tcgctacagc agcagtacat cggtgagtac cagtaaaggc ggtggcacgg gcttcagcac
     3301 tggcgtgtcc ggctcgctga atgaccgttt cagctatagc ctgggcggcg gtcgtgacag
     3361 cggtggtggt gtgagtagtt atctaaacgc gtcgtacagt ggtgaccggg cttatctgaa
     3421 tggtgccctg aaccactcac agtccggcgg aaccagtggt tctgtatcag tcagcggttc
     3481 ggtactggtc gtcccgatgg cgaaagacat tatgttcagc cgcacgaccg gggacaccgt
     3541 ggccgtggtg aacgtgaagg atacaccggg tgtgaaggtg acgtccggtg acggacagac
     3601 tgacagtgac ggcaacctgg tggtaccgct gaacagctat gactggaaca cggtgacgat
     3661 tgatgcgggc acactgccgc tgagcaccga actgacgaac accagtcaga aggtggtgcc
     3721 gacggacaag gcggtggtct ggatgccgtt tgatgccctg aaagttaagc gttacctgct
     3781 gcaggtgaag cagcgtgacg gtgagtttgt gccaggggga acctgggcac gtgacagtaa
     3841 gaacacaccg ctgggctttg tagctaacaa tggtgtgctg atgattaaca cggtggatgc
     3901 gccgggtgat atcaccctag gccagtgccg gatacctgcg gccagactgc aggatactga
     3961 gaaactacag gagattacgt gtgagtaagc gtaacgcagt aacgacgttt ttcactaacc
     4021 aggtgacaaa agcactggga atgacgctgg cgctgatgat gagctgtcag agtgcggtgg
     4081 cttccctggc ggcagaccag acccgctata tctttcgcgg ggacaaggat gcgctgacca
     4141 tcacggtcac caacaatgac aaggaacgta cctttggtgg tcaggcatgg gtggacaata
     4201 tcgtggagaa agacacccgt ccgacttttg tggtgacgcc gtccttcttc aaggtgaagc
     4261 cgaatggtca gcagacactg cgtatcatca tggcctcgga ccatctgccg aaggataaag
     4321 agtcggtgta ctggctgaac ctgcaggata ttccgccggc tctggagggc agcggtattg
     4381 cagtggcgct gcgcacgaaa ctgaaactgt tctatcgccc gaaggcactg cttgaaggtc
     4441 gcaagggggc agaagaaggc atcagtctgc agagccgccc ggatggcagg accatgctgg
     4501 tgaacaccac gccatacatt tttgcgattg gcagcctgct ggacggaaac ggaaagaaaa
     4561 ttgccgcgga taacgagacg gcgcagaaac tgctgatgtt catgccgggt gatgaagtac
     4621 aggtgaaggg aaatgtggtg aaagtggact ctctgaatga ttacggtgaa ctgcagacat
     4681 ggacgattaa ccagaagaaa tcagcaatat cgtctgcaca gaagtcatct gatacagagg
     4741 gtaattcatc tgataaaaaa taatcgccct ccggcatacg gaacagaggg gtaataaatg
     4801 aagaaaacaa tgatggtagt cgccttggcc ctgagtgcac tcagtattca gtcaacactt
     4861 gccgctgaat acagcgagaa agcgcagtat ctgggggtgg tgaatggcca ggtggtgggt
     4921 aacagcgtgg tgaaggtaac ccgcataccg acagatccgg tgctgtaccg gagtggagac
     4981 acgacgcctc ttcccgaccg cctgaccatc cgtaatgcag aatccagagc agcatctggt
     5041 ggcttggcgt atatcacggt gaaacaggtg ctgccggata acggggaagc ccgcatcacc
     5101 ctgaagacgg ccctgatggt tgacggaaag aaagtggcaa tcagcgccag acagcagggt
     5161 gaggatatgg tgataacttt gccggaagca cagaagcaga ttgaattaag aacggatgca
     5221 ccggcagagc tggaagtacc ggtcagttac cggggaaatc tgcagatagc gctgcaggtg
     5281 gaggactgag gattcatttc ctttataaat gcagtacatc ccgcgatacc ggagcgggtc
     5341 atattggtaa agggatttac cgtcttttaa aacgggaata acgtaaaact attctgagtt
     5401 aaacactgtg tttaatgaaa tgcggttatt taaatgaatc cgcaggggat ggttttgcgg
     5461 taattccggg aaaataagga ttaccgattc agtttattat ttatgaaacg tcaaggtgat
     5521 tattttatga aaaagactct gattgcactg actgtggctg cttctgccgc agtatccggc
     5581 tctgctatgg cgtggaatcc ctctggcctt ggtggcaacg gtggcacctt tagcatgagt
     5641 ggtactctga gcaaagctga gaaaacaata ccgtgggcag tggaaatagg tgctccggta
     5701 acagcgcttg atgtgaccgt taaacctgac cagaagaatg ttttcattct tttagaaaaa
     5761 gacataccga tacttggcat tcgtagcaac ccgactggtt tcatggggga aaagggtatt
     5821 gccccacaaa tcgattataa taaagccgct gatgtggatt atatggataa ggggaattta
     5881 cctgcgcaag ttaatgtcac atcatctgat ggtaagaaaa ttggtactct ggcgttcgta
     5941 atgcgagttg cctgtcaggc cagcgaaaag aggggtgaag gcgacgacaa gttagagagt
     6001 aacatgctat tcgccagttc aaaaggtaaa gctttctttg gtggagttgc caaagatgac
     6061 aaaggtgtct ggagtgacgc cgctgcctat acttgggcta cccacctttt tagcagtatt
     6121 gctaactcgt gggatactaa aattccttac acggcgggcg atacagatga aaccgacttc
     6181 agtaatggag acaggcttta ccgaggttac tatgcatccg gccttgccaa ggatatcccc
     6241 atggaactag ttcttgatca gactataacg tctgctaccc cagttccgtg gaaagcttct
     6301 ctgccaattg ttatctccta catgtaattc agcagtgttc agcagtaagc acttacccgt
     6361 ttactgctga ttaaaacttc agaacacagt ttccggtgac ccgacctgaa tatacagaac
     6421 cggtatgtca gaacaggtgt tttttctgtt ggagtgtctt gtgacgcata ttggttctgt
     6481 gcttttttta acaaaattat tttattgagc tgttttgttt tatccggata aacaggaatg
     6541 aatgccgatg aaaataacgc atcattataa attcttcctt tctgctgtta ttttggtaac
     6601 aatattttat tcggcagtgc catatgcaaa tattcttgat ggcggcgaaa ttcagtttaa
     6661 tggctatgtt actgatgaag cacccaaatg ggcctggcag ataagttctc ccgaccagac
     6721 ctggtctgtg gatactgccg atgcccgtac agagaacggg caactggttt ttaatttgca
     6781 tgataaaggg gctctgccat ttctggaggg acatctgcat gatgtggctg agcgcggtgg
     6841 tcccggattc acgccattta ttactttcag cagtaacgga cagcctttta ctgtgacgga
     6901 aggaaatggt gcctcagcac agcactttcg cgcctctgtc ccggtgcgtg acccggagac
     6961 ggggaacgtg tcgggacagc ttttttttac cctgaatcag ggaatggcgg tcagtaccgg
     7021 cagacaggac gacggggcat ccgtaccggc tggcatgtcg ctggtcagtg gacagagtgt
     7081 gacggatgta cagccgggaa gtctgccaca ggggctgaaa gcccgactct cttccctgtt
     7141 actgatgaat cagaattttg gcaatggcat gaatgcggtg gataacggac aggttatcaa
     7201 ccaggggata ctgactgacg gtcgggtgat ggatctggct gcggcatatg cctctgaggt
     7261 gtcggatttt gaactgcggt tgccggcaga gggtacacct gcagcatggc aggcggggct
     7321 gaatgtgacg gttacagtac agtgatggtt gcagggatat aaggagagaa gagaatgaaa
     7381 aaggtgacgt tgtttctgtt tgctgccagc cacctgcctt cctgtgtact ggcctggaac
     7441 acgccaggag aaaatttcag cggagagctt aagctggaag gcccggtgac cagtacccgt
     7501 aacccgtggg tatggaaagt cgggcaggga aatggaggtc tggaggttaa acagagtggc
     7561 ggtggggggg ggattcaggt cgcactgccg gcgatgaccg ttttactggg aaaaaccacc
     7621 ctgaccacac cggcaggacg tgaggggctt tcaccccggg tcagttacgg aaaaggcact
     7681 gaggggtttt cacttgtatg gacagcgcct ggcatggctg aagtgacgct acctgtcacc
     7741 ggagataata atgcccgggc aggcacattt gttttcagaa ttcaggctgt gggggtgttg
     7801 cgccatgtgc agggcggaca accggtgtat gttggtatgt atgacgacct gaatacgaat
     7861 ggtttgccag gtgaaagcac tgccatgaag gtttctgata ttccgggcgt tctgcagaag
     7921 atgttcagtg gggaaggccc cggctggctt cagacaatga cactcagtgg ttattcaggg
     7981 gtgagtcatt tcagtgatga cttcctgcgc caggttgaag gcgtgtatgg tgcacagact
     8041 gtggctggca gtggtgaatt acggctgaac ggcgagatgc cggaacactg gagggtgtca
     8101 ctgtcagtaa acattgaata tcagtaagga gcaggagtgc gttttataat ccggagataa
     8161 ggaacgtcgg ttactgtaag attggggagt tgtgttgata gcgggagtgg ctggaataag
     8221 tggtatacat agataaaaat taaaactatc actatttgag tgagtttttt cctgctctcc
     8281 atgggcttta tagctcacaa aaaatagaat aatattatta attattctgc ttttttatgg
     8341 gcattccccc ttgaaaaatg agtttgtgcc agaatattat taatatgttt tttgtgattt
     8401 actaatcaag gattgctgaa tagcatccag tactgaatat tttgttcaat tatgtcagga
     8461 accataccga aaatcaaaac aggtgaaaat gttctgtctg cagccattca gcgcattgaa
     8521 tggctatttg gaacgtttcc ttctgtctgc ttgtcctttt ctggtggtaa ggattctacc
     8581 gtgcttttcc atcttgtgtc cgaagtggcc cgcaaaaaga ggcgtcgttt ttcggtattg
     8641 ttcattgact gggaagcaca atatcaatgc accattacgc atattcagaa gatgcagaag
     8701 atgtacctgg atgtgacgga caggttttac tgggtggccc ttcccctgac tacggtaaac
     8761 ggtgtctctc agtttcagcc ggagtggata tgctgggagc ctggtgtgac ctgggttcgc
     8821 catccaccgg aatatgctat tacggatatg gcgtattttc ccttttaccg atatgccatg
     8881 acctttgagg aatttatccc ggcgttttcc tcctggtttg ccagggataa atacctggtg
     8941 gctgttctga ccggagtccg ggcagatgag tccctcaacc gctttatggg actggtatcc
     9001 cgtcgcaaac agagatatgc cgatgataaa ccctggacta cggcatcacc tgaagggttt
     9061 tattacacca tgtatccgtt gtatgactgg aaagcccgtg atatctggat atataacgcc
     9121 agaacccgag ctatctacaa tcccctgtat gacctgatgc accgtgcctg tgtgccgtta
     9181 cgcaacatgc gtgtctgtga gccttttggt cctgaacagc gtaaggggct gtggctttat
     9241 catgttctgg agccggaaac ctgggccagg atgtgtgagc gggtgtcggg tgctgccagc
     9301 ggggcgcttt atgccaatga gagcggtgcc tattttgccc tgcgtaagcg tatcacaaag
     9361 ccgcctcatc atacctggcg tagctatgcg atgttcctgc tggatgtgat gccggaaaga
     9421 acggcagaac attaccgtaa taaaattgct gtctacctgc gctggtatca gacgcggggc
     9481 ttcccggatg acatcccgga tgaacaggag aatgacctgg ggagccggga tatcccgtcc
     9541 tggcgacgta tctgtaagac actcataaag aatgatttct ggtgccggac tctctccttc
     9601 agtccgaaca aaccccggca ctatgaacgt tacctgcagc gtatgaagga aaggaggaag
     9661 gaatggggga ttctgtgaca ccggagatac ttggcaacat gattcgtcag tatttcagtc
     9721 aggtacgaac tgaaaaagag agcatacagg ctctgaatca tctgcgcaga gtgcttcatg
     9781 aggtcagtcc ttttgcacag gagcctgtgg actgtgtgtt gtgggtgaaa gcggatgagg
     9841 tggtggccaa cgattacaac cctaatgtca tggcactagg tgagaaaaaa ctactgaaac
     9901 agtctctgga gaaagatggt ttcacacaac cggtggtggt gtcagaggag aagaatcatt
     9961 atctggtggt ggatggtttt catcgtcagt tactggggcg ggaagcagat acaggaaaac
    10021 gcctgagggg ctggttgcct gtagcctgta ttaatccgga gagaaaggga caggccgcac
    10081 gtattgccgc gaccattcga cataaccggg ccagggggaa acaccagatc actttaatgt
    10141 ctgatattgt cagggatctt tcacgtctgg gctggacgaa tgaacgtatt ggtacagagc
    10201 tgggaatgga ccaggatgaa gtgctgaggc tgaaacagat tagcgggctg acagagttgt
    10261 ttcaggaaga ggatttcagc tcggcttgga cggttcgttg atgttgttaa atatgggcgg
    10321 tatttctgaa agttgatact ttgggtagaa aaaatgatac cgaaaactat ggaggatgtg
    10381 cttgtcaggt gactggacac cataggtctt aggctttgat aacgggataa gatgagctgc
    10441 ttttctcata ccatcaggtg tgatgatggc gcagatttac aaaatctgat tgattctaaa
    10501 gatgtataag taaaaatcaa gctttgatat gaaaaaacag atatttatta ataataaacc
    10561 tcctgtagtc ccttactcag ggacgcacgc gaaaatattt aaatacattg aaatccctct
    10621 gccgtttttc tactttatct atacatcagg cgaaccattc cacatatctg tccaaaatac
    10681 tgttatttat gtatcaaaat acaatggtat atttattaat aaattagttc ctttttctct
    10741 tttatttgac agggatatat ctgttcttca aagaagagat atttgtgttg tgcgatttac
    10801 gtcagaggaa ataagtgaac ataacgtttt atttgatcat gacattgaac gattaaaaaa
    10861 aatttccaaa gctcagctta tatctcccga ttatgttttg attgattttt ctagtggggg
    10921 gggagatgaa ccctatgcaa tgtcctggtt aatatctcag tgtgctcatc agtgcactga
    10981 taataaaaag acggagacag atgccattta cgataaagtt cggagttctt atttgctttc
    11041 atgtattctg aaaaaaaaca aaaatgttgg gcttattctt catgcaccat catttgtttc
    11101 tgtgtctgaa aaaattgcca gaattgtgat ggcaaattac tcccgtaact ggagtaattc
    11161 cgagttggcc agtgcggttt taatgagtga atcttcttta aaaagaagaa tgtacaaaga
    11221 ggtcggttct ataagtacat ttgtacataa aataaagctt acagaagcta tcaggaaatt
    11281 acgaaggaca aatactccca tttctgtcat atcatctgag ttaggttaca gttcaccatc
    11341 gtatttttct aaagttttct ttaaatattt aaagacatac cctcaaaaca taaggaagaa
    11401 aaatggtcgg taatgtcacc aattaggagg acttgcagag ccgctttttt acgatactca
    11461 ccccgtaagt aagcgataat tggtaactta aacgcccatc tgacatagat ggacacctga
    11521 gtatggaatt acaggactgg cgaaaagaac ctcgtaaaaa gtatttggtg caaatcgttg
    11581 gttcctttgc tgaatttgaa cgcgccatgc ttcgtgaacg gacaaagagt ggacttgcag
    11641 cggcacgcca ggacgggttg gtggatggcg actaaagtta acaccccagc aacaaaaaga
    11701 gattgtctcg ctcgtcacat cggggcagaa aacaggtgct gatgccgccc gtctgtttcg
    11761 ggttcaccct tccaccgtgg tgcgtcttct cgccagacac cggatgactg agatcaaaca
    11821 gacataatct atgcggagat ttacagcagg gcatggttca caaatctgac agcgatgaat
    11881 tatcggcact gtgggcagaa aatgctcgaa tcatcaagcc cctgttacct cctgaacccg
    11941 ccacaccacg ggccggac
//
LOCUS       DADLSA010000114        11872 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.114, whole genome shotgun sequence.
ACCESSION   DADLSA010000114 DADLSA010000000
VERSION     DADLSA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.114"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            1..1209
                     /locus_tag="JN691_003068"
                     /pseudo
     CDS             1..1209
                     /locus_tag="JN691_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            1432..1929
                     /gene="ubiC"
                     /locus_tag="JN691_003069"
     CDS             1432..1929
                     /gene="ubiC"
                     /locus_tag="JN691_003069"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAY7244451.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1942..2814
                     /gene="ubiA"
                     /locus_tag="JN691_003070"
     CDS             1942..2814
                     /gene="ubiA"
                     /locus_tag="JN691_003070"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAY7244452.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2969..5392)
                     /gene="plsB"
                     /locus_tag="JN691_003071"
     CDS             complement(2969..5392)
                     /gene="plsB"
                     /locus_tag="JN691_003071"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAY7244453.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            5563..5931
                     /locus_tag="JN691_003072"
     CDS             5563..5931
                     /locus_tag="JN691_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAY7244454.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            6041..6649
                     /gene="lexA"
                     /locus_tag="JN691_003073"
     CDS             6041..6649
                     /gene="lexA"
                     /locus_tag="JN691_003073"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAY7244455.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6668..8047
                     /gene="dinF"
                     /locus_tag="JN691_003074"
     CDS             6668..8047
                     /gene="dinF"
                     /locus_tag="JN691_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAY7244456.1"
                     /translation="MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNGTWFAAT"
     gene            8163..8372
                     /locus_tag="JN691_003075"
     CDS             8163..8372
                     /locus_tag="JN691_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAY7244457.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(8414..8929)
                     /gene="zur"
                     /locus_tag="JN691_003076"
     CDS             complement(8414..8929)
                     /gene="zur"
                     /locus_tag="JN691_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAY7244458.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            9247..9501
                     /locus_tag="JN691_003077"
     CDS             9247..9501
                     /locus_tag="JN691_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244459.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            9525..10232
                     /locus_tag="JN691_003078"
     CDS             9525..10232
                     /locus_tag="JN691_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HAY7244460.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            10595..10717
                     /locus_tag="JN691_003079"
     CDS             10595..10717
                     /locus_tag="JN691_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAY7244461.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDRMYRF"
     gene            10953..11525
                     /locus_tag="JN691_003080"
     CDS             10953..11525
                     /locus_tag="JN691_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SocA family protein"
                     /protein_id="HAY7244462.1"
                     /translation="MFCEEKVAQMAAYLLLKRGGRMAYLKLMKLLYLSNRQSILKHGR
                     MIGEDSLYSMKFGPVMSNTLNLIRGKAEGIGDYWYNLIETNGHNVSLRSDPREMDADE
                     VFDELSRADIRILDEIYSRYGHMNRFDLANMTHLESVCPEWHDPGNSRKPIDLKEMLI
                     SEGKSEDEANRIIGKMEESQKLKEFSLQLS"
BASE COUNT         3042 a         2595 c         2978 g         3257 t
ORIGIN      
        1 tcttttttat catcaaatct acaacatgta actttcgaat gtaaagaaat tgagaattgc
       61 aattttacta aggcaacagt tgataatgtc atatttaaat gtcgacgttt acacaatgtg
      121 atttttctca aaacgagtgg cgaatgtgtt gattttagcc aaaatattct tgatacagtt
      181 gacttctcgc agagtcaact tggtcatagt aattttcgcg aatgtcagat tagaaattca
      241 aacttcgata attgttatct ttacgcttcg cacttcacca gagcagagtt tctgtctgcc
      301 aaagaaatat catttattaa atcgaatttg acagctgtta tgtttgatca tgtgcgaatg
      361 tcgacaggga atttttaaga ttgcattaca gaacaattgg aattaactat tgattattca
      421 gatatatttg ggaatgaaga tctcgatggt tatatcaata acattataaa aatgattgat
      481 acattgccag ataatgcaat gatattgaaa tccgttctgg cagtaaaact ggtgatgcaa
      541 ttaaaaattc tcaatattgt taataaaaac tttattgaga atatgaagaa aacatttagc
      601 cattgtcctt atataaaaga tccaattata cgtagttata tacattctgg ggaagatgac
      661 aagttcgata attttatgcg tcaacatcga ttcagtaagg tgaatttcga tacccaacag
      721 atgatccatt ttattaacag atttaatatg aataaagggc tggttgataa aaataacaat
      781 ttttttatcc aacttatcga tcaggcctta cgatcaacgg atgatatgat caaagcaaat
      841 gcctggtatc tttataaaga gtggattcgt agtgatgatg tttcacctat atttatagaa
      901 actgaagaaa agttaagaac ctttaacact aataaattaa cacgaaacga taatatattt
      961 atcctgttct cctctgtcga tgatgggcct gttatggtgg taagctccca gcgcttacat
     1021 gatatgttga atcctacaaa agataccaat tggaattcca cgtgtatcta taaatccaga
     1081 cataagatgt tgcctattaa tcttactcag gaaacacttt tcagctccaa atctcatggt
     1141 aaatatgcgc ttttcccaat ttttactgcg agttggcgag ctactcgtat aaagaataaa
     1201 ggtatttaag taaaggaaaa catcaccgtt cctagcatca tgggcggtga tgcccctatg
     1261 gttgccctcg ccagcacggg catcggcaaa gcgtaaggtt caacagcgtt ttaccacttc
     1321 atacgattgt tgcgtttttg ttgcgcatta gatcacttaa tttgctttac atctcccgta
     1381 aacacttttc tgcgatacaa tgcctttaag ttatgtaacg gagagttcgg catgtcacac
     1441 cccgcgttaa cgcaactgcg tgcgctgcgc tattttaaag agatccctgc cctggatccg
     1501 caactgctcg actggctgtt gctggaggat tccatgacaa aacgttttga acagcaggga
     1561 aaaacggtga gtgtgacgat gatccgcgag gggtttgtcg agcagaatga aatccccgaa
     1621 gaactgccgc tgctgccgaa agagtctcgt tactggttac gtgaaatttt gttatgtgcc
     1681 gatggtgaac cgtggcttgc cggtcgtacc gtcgttcctg tatcaacatt aagcgggccg
     1741 gaactagcgt tacaaaaatt gggtaaaacg ccgttaggac gctatctgtt cacatcatcg
     1801 acactaaccc gggattttat cgagataggc cgtgatgccg gactgtgggg gcgacgttcc
     1861 cgcctgcgat taagcggtaa accgctgttg ctaacagaac tgtttttacc ggcgtcaccg
     1921 ttgtactaag aggaaaaaaa tatggagtgg agtctgacgc agaataagct gctggcgttt
     1981 catcgcttaa tgcgtacgga taagccaatt ggcgcgttac tgctgctctg gccaacatta
     2041 tgggcgttgt gggtggcgac accgggcgtt cctcagctct ggatcctggc ggtgtttgtc
     2101 gcgggtgtct ggctgatgcg cgctgctggt tgcgtggtga atgattatgc tgatcgcaag
     2161 tttgatggtc atgttaagcg cacggcgaac cgaccacttc ccagcggcgc ggtaacagag
     2221 aaagaggcgc gcgcgctgtt tgtagtgctg gtactgattt cgtttttact ggttctgacg
     2281 ctgaatacga tgaccattct gttgtcgatt gccgctctgg cattggcgtg ggtgtacccg
     2341 tttatgaagc ggtataccca tctcccgcaa gtggtgctgg gcgcggcgtt tggctggtcg
     2401 attccaatgg cttttgccgc tgtgagtgag tcggtgccat tgagttgctg gttaatgttc
     2461 ctcgccaata ttctctgggc ggtggcttac gacacgcagt atgcgatggt tgaccgcgat
     2521 gatgatgtga agattggcat taaatccacg gcaatcctgt tcggccaata cgataaattg
     2581 attattggta ttttgcagat tggcgtactg gcactgatgg cgatcatcgg tgagttaaat
     2641 ggcttaggct ggggatatta ctggtcaatt gtggtggctg gcgcgctgtt tgtttatcaa
     2701 caaaaactga ttgccaaccg cgagcgtgaa gcctgcttta aagcatttat gaataataac
     2761 tatgttggtc tggtactgtt tttaggactg gcaatgagtt actggcattt ctgatgatgt
     2821 aaaaaagccg gatgatcatc cggctttctt ctgggttgcc tgatgcgctg cgcttctcag
     2881 gcctacccgg tgaatcgcaa tttattgaat ttgcaggtca tggtaggccg gataaggcgt
     2941 tttcgccgca tccggcaatt ctctctgatt acccttcgcc ctgcgtcgca ctctcaatcg
     3001 tcaaacgcac gtctgatgta atcaactccg ccagcaactg ataaaccttc atcgtttctg
     3061 ctggttcggc atcgccgcta tcgctgatat acccttcatc acgcagcgtc agcaccagag
     3121 aactgaacac cgccttgtcg aagaactccg gcgcgttgat gccgtgcagc acggagagac
     3181 gttgcgcaac ggtgcggctc tctttctcca gcgtaccgcg gttgatcgac gggttggcac
     3241 tcaacaacca gaaggtgatg gcataacgtt gcagcgtttc gcgcgcgcct gcggccagca
     3301 gctgtagggt gcgagaatgc gccgggttga tatgcaactc atcatcttgc agggtaatca
     3361 gcccctgacg ttgcatctca tttgccagcg catcaataac gtccggcaac tcgtcgcgat
     3421 cccagcgcag gaacagctcc gctttcagca ttgggtaaag cacattgacg tgctccatca
     3481 atacgtcgcg ggagatgtgg cgatgctggg tgacaattgc cgccatcagc gaaggcagca
     3541 ccaacatatg cgcaatgttg ttgcgatagt aggtcatcag caccgcttgc tcgcgtggca
     3601 gaatgatgat gtcgccgata gtgtctttct cgacttcaaa cttgttcatt tgcagcgcgt
     3661 gatcgataag ctcgctggcg ctggctgaag gaacggtaga gtccgtggag taaggtacgt
     3721 tgcgcatcag atccaggtag cagttgagtt gctcggttaa ctgctcgcgg gtgagtgagc
     3781 gctgacgtga tgccagtagc gcagtacagc acaggttcat ggcgtttgcc gcgcctgcgt
     3841 tgttaatgcg taccatcaga tcggcagcaa tattattgac cgtcggcgtt aaccaggccg
     3901 gacgcaccgc ttcgatggga tcgatagatt cacgccagtc tggtacgtgc tggttaaggt
     3961 aggtcatcaa cggcattggc tcaccgaagt tgacgtaacc ctgaccgaga ttacgtagct
     4021 tgcttaaacc gcgcagcatc tgcggcaggc tctctttctc tttcgtcgcg ccgcgcagtt
     4081 ctttggcgta agtacccact tccatgacgt gctcataacc gatatagatc ggaatcagcg
     4141 taatcggacg cgtgccgcca cgcagcatcg cctgaatggt catcgacagc gtaccggttt
     4201 tcggatccag caaacgcccc gtgcgggaac gaccgccttc cacgaagtac tcgacggaat
     4261 aaccacggct gaacagttcg ccgagatact cacggaaaac ggtggaataa agtttattgc
     4321 ctttaaaagt acggcgaata aagaacgccc ccagacggcg gaaaatcggc ccggccggcc
     4381 agaagttcag gttgatcccg gcggcgatat gcggcggcac cagcccctgg tggtacagca
     4441 cgtaagaaag tagcaggtag tccatgtgac tgcggtggca aggcacatac accagctcat
     4501 gaccgtcgtg ggccagctgg cgaacgcgct cggcgttatg gacgttgatg ccctgataaa
     4561 atcggttcca ggtgaagccc agaatgcggt cagtcaggcg gatcatctcg taagagaaat
     4621 tcgccgcaat ctcttccatc agcgcaatcg cgttctgctg cgctttttca tgggagattt
     4681 ttttgctgcg cgcttcatct tctaccgctt tggcaatggc gcgggaggcg agcagcttat
     4741 taaacagatc ctgacgagca ggcagacgtg ggcctacggc agccagacgt tgacgggcaa
     4801 agtgcatacg cgccacgcgc gccagtttct gagcgatagt tttatccgtg ccgtgttcat
     4861 ccgccatacg gcgcagcgac actgacggcg agaaacgcac aaaactgtcg cgaccgagcc
     4921 acagtacagc gaaaaatttc tgtacgccgt taagcatacg cagcggcggg ttcacttcgc
     4981 ctttttcacg ccccggcgcg cgaccaaaca tcaccgatac tggcaccatc tgcacatcca
     5041 gatttgggtt gctacggtgc aaatcgagat agtcgtggaa aagcttaata gactcttctt
     5101 tcggcgtgta ataggtgaat acacgcggcc cgccgtgaat gaacacatag cgcggcagta
     5161 gcgtgccgtc gatttccagc ggctctaacg ggtcaggcaa atcatgtgcc agacactggg
     5221 cgcgcaacgt cagcaaatct gctttcgagt tgtacggtaa aacgtacata attggacgag
     5281 aggtatccag ccccagttcc ggggcaggat ctgccggaat agacttgctt tttaccagga
     5341 tgcttaatgg taaattcagt aatttgtagt aaattcgtgg ccagccggac ataaacgatg
     5401 taaagcctct ggttaataat gcaaatgcgc ggcaaggata gcagaaagtc atgggaaatt
     5461 ctgtggtatc cgctcatgtt tcgcgcggcg ttacgcaaac ccgaatcatc ggatttaacg
     5521 gtacactgat attgacgctc ataatgtaaa aaggttcttt caatggccaa taataccact
     5581 ggattcaccc gaattatcaa agctgctggc tattcctgga aaggtttacg cgctgcatgg
     5641 atcaacgaag cggcattccg tcaggaaggc gtagcggtat tgttggcggt ggtcatcgcc
     5701 tgctggctgg atgtggacgc aattacccgc gtgctgctta tcagctccgt gatgctggtg
     5761 atgattgtgg aaatcctcaa tagcgccatc gaagcagtgg ttgaccgaat tggctctgaa
     5821 taccatgagc tttccggacg cgcaaaagat atgggatccg ccgcggtgct gattgccatt
     5881 atcgtcgccg tgattacctg gtgcatcctg ttatggtcgc attttggata acccttccag
     5941 atttcgataa atctctggtt tattgtgcag tttatggttc caaaatcgcc ttttgctgta
     6001 tatactcaca gcataactgt atatacaccc agggggcgga atgaaagcgt taacggccag
     6061 gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta tgccgccgac
     6121 gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg aagaacatct
     6181 gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac gcgggattcg
     6241 tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg ccggtgaacc
     6301 gcttctggcg caacagcata ttgaaggtca ttatcaggtc gatccttcct tgttcaagcc
     6361 gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg aaagatatcg gcattatgga
     6421 tggcgacttg ctggcagtgc ataaaactca ggatgtacgt aacggtcagg tcgttgtcgc
     6481 acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa cagggcaata aagtcgaact
     6541 gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac cttcgtcagc agagcttcac
     6601 cattgaaggg ctggcggttg gggttattcg caacggcgac tggctgtaac atatctctga
     6661 gaccgcgatg ccgcctggcg tcgcggtttg tttttcatct ctcttcatca ggcttgtctg
     6721 catggcattc ctcacttcat ctgataaagc actctggcat ctcgccttac ccatgatttt
     6781 ctccaatatc accgttccgt tgctgggact ggtcgatacg gcggtaattg gtcatcttga
     6841 tagtccggtt tatttgggcg gcgtggcggt tggtgcaacg gcgaccagct ttctctttat
     6901 gctgttgctg tttttacgca tgagcaccac cgggctgact gcgcaggctt atggtgccaa
     6961 aaatcctcag gcattagccc gtgcgctggt gcaaccgttg ctgttggcgt tgggggccgg
     7021 ggcgttaatt gcgctgttgc gtacgccgat tatcgatctg gcgctgcata ttgttggcgg
     7081 tagtgaagcg gtcctggaac aggcgcggcg ctttcttgaa atccgctggt taagcgctcc
     7141 ggcgtcgctg gcgaatctgg tattactcgg ttggttactc ggcgtgcaat atgcccgtgc
     7201 gccagtaatt ttgttagtgg tcggcaatat cctcaacatt gtgctggatg tctggctggt
     7261 gatggggctg catatgaacg tgcagggcgc ggcgctggcg acggttattg cggaatatgc
     7321 aacattgctg attggtctgc taatggtgcg taaaatcctc aaactacgcg gaatttccgg
     7381 cgaaatgctg aaaactgcct ggcgaggaaa cttccgtcgc ttgctggcgc ttaaccgcga
     7441 tatcatgctg cgttcgctgt tgttgcaact ctgtttcggc gcgatcaccg tacttggtgc
     7501 gcgactgggg agtgacatta tcgctgttaa cgcggttctg atgacgctac tcacctttac
     7561 cgcctatgcg cttgatggtt ttgcctacgc ggttgaagcg cactccggtc aggcatacgg
     7621 tgcgcgcgac ggtagccagt tgctggatgt atggcgggca gcgtgccgcc agtcggggat
     7681 tgtagcgtta ctgttttcgg tggtttattt gctggcaggg gaacacatca ttgcgttgct
     7741 gacgtcgtta acccagattc agcagctggc tgaccgctat cttatctggc aggtgatttt
     7801 gccgttggtt ggcgtctggt gttatctgct ggacggcatg tttataggcg caacgcgtgc
     7861 cgccgaaatg cgtaacagta tggcggtggc cgccgcaggt tttgcgctga cgctccttac
     7921 gctgccgtgg ctgggtaatc atggtttgtg gctggcatta accgtctttc tggcgttgcg
     7981 cgggctttct ctggcggcta tctggcggcg tcactggcgc aacggtacct ggtttgccgc
     8041 aacgtgacgg ttaaaaattc tgaataaatt atcctaagcc aaattgctga ctacacttaa
     8101 tctcacgttc agaagaaaag tgaacgtact ctcattcaca acctaacgat gaggtcttga
     8161 ttatgaataa agatgaagcc ggcggtaact ggaaacagtt taaaggtaaa gtgaaagagc
     8221 aatggggcaa actgaccgat gatgatatga cgatcattga aggtaaacgt gatcaactgg
     8281 tcggtaaaat ccaggaacgt tatggttatc agaaagatca ggcagaaaaa gaggtcgtgg
     8341 attgggaaac ccgcaatgaa tatcgctggt aattaatccc tcctgcccga cgtgtacaag
     8401 gatgtacgcc ctcttaacgc ggtttctttt tcacctgcac agagtgatca tgctggcact
     8461 gttcaggatg acgacacgct tccacttcca cgcatgccgc acagagccca tgtgcttcaa
     8521 tcacattatg ccgcagggca aaccccattt ttgccgccag cgtatgcata atgtcttcca
     8581 cgccttctgc acactcttct ttcaccgcgc cgcagcgatc gcaaataaac atggctgacg
     8641 tatgcgtggg ctggtcgaac agatgacaga gcacataact gttggtggat tccaccttat
     8701 gcacaaaacc ttgctcaagc agaaaatcca gcgcgcgata aaccgttggc ggcttggctt
     8761 gcggttcagc ttcgcgcagt aaatcaagca gatcataagc gctgatagcg ccatcctgca
     8821 gactcatcag gcgcaacact tccaggcgct gtggggtcag gcgcacatta cgctgcgcgc
     8881 agattttttc agcctgcgct aataactcct gcgttgtggt cttttccatc gggcacctca
     8941 aagtgggggt taagaatgtc cttactttac catgttccag gaaaaacacc gagaaccctt
     9001 attgttgccg taatgttgat tttctgtttt gtaggtaagg tgttgtgttg ccttgtcgta
     9061 ccattatcaa cacgataata attaataatt catttttaaa taaattgctt caagttaact
     9121 cgctatttat ttaactgggt ttaaacgcat acctgtagtt cagaaattat gagcgatttt
     9181 tgatagtgac cagatggcaa aagaattata aggaggacac ggtacgacaa aaaaggtaga
     9241 tgaatcatgt tgaagataat acccggtgca actggatact ttaataaaac actcaattca
     9301 aatcaattcg aaaataaaga tgcaaccaaa gataaattgg ataatagagg ttcaatcaaa
     9361 ggtaaattaa ataatatata tggtaaatct attgattatg cagcgctgcg tcatcgggat
     9421 ataattattg caaaaataga tttgtttatt caacgaatca cgcataattt atggcatgca
     9481 cgtaaaaaaa tgtgttttta attgagcaaa taaatgatct caaaatgtgg gtcaataagt
     9541 atactgacga ttgcactgat gaggatttaa acgatcgtga ctttattgca tcagttgttg
     9601 accgggctat ttttcatttc gcgattaata gtatatgtaa tcctggggat aataaagatg
     9661 caatgcccat tgaacaatgt acttttgatg tagaaactaa gaatggcctt ccctccacgg
     9721 ttcagctatt ttatgaggaa tctaaggata atgaaccttt agcgaatata cattttcaag
     9781 caataggttc tggtttttta acgtttgtta atgcctgcca ggaacatgat gacaacagct
     9841 taaaattatt tgcttcgctg ttaatttcac tttcatattc tagtgcctac gcagatttat
     9901 cagaaacagt gtatattaat gaaaataatg agagctacct gaaagctcag tttgaaaaat
     9961 tatatcaacg tgatatgaag aagtacctgg gagagatgaa gcgtctggca gatgggggag
    10021 aaatgaattt tgatggctat ctggataaga tgtcacatct ggtgaatgaa ggaacgctcg
    10081 atcctgatat tttaagcaaa atgcgagatg ctgcaccaca attaattagc ttcgcgaagt
    10141 cgtttgaccc aacctcaaag gaagagatta aaatacttac agacacttct aaattaattt
    10201 atgacttgtt cggggttaaa tcggagaaat aatatgtgaa gttcttcgat agtatggaag
    10261 gccttatata aaaggaccca atatttattg ggttcttttt tctctatcaa tgctattagc
    10321 agggagatat atcaccagag tttaatgtgt gattttttat ttatcgtcga acatggattg
    10381 tttatcattg gccttaacaa agttaacggc taataagatt atttccatca cttcgtgaga
    10441 gcttcatgcc ttgagaggat ctcaattttc ttttgcaatg agacaggcgc ttcctgttgt
    10501 tatggtatag taccccgcta ttgagcctcc tgaacagtga agctgaataa cataacccca
    10561 tgatatatcg ataaaataat ctctacattt gaaaatgcac ggtaattctg aaatgcaaaa
    10621 aatcaaccaa accagcgcaa tgcctgaaaa aactgacgtt cactggagtg gtcggtttag
    10681 cgttgcacca atgctcgata ggatgtaccg tttttgaaaa acaactagtt atacactttg
    10741 tgggagccta ttgggaacct gttattctta gaaagcatca tttgctcatc acttcaattg
    10801 accatggtta aagccagcta taaacttgac aaacgaggta actggttgct taggcccaag
    10861 tgggcacacc ataagaagct catgttggtg atgaagcaac ccatgaagta tcgagaataa
    10921 ttataaagtt cactatcttt gaggtttctt atatgttttg cgaagaaaaa gtagctcaaa
    10981 tggctgcata cctacttctt aagagaggtg ggcgcatggc atatctgaaa ttgatgaagc
    11041 tgctctatct gtctaaccgc cagtcgattt tgaagcatgg caggatgatc ggcgaagata
    11101 gcctttactc tatgaaattt ggaccagtca tgtcgaatac gctgaacttg attcgcggta
    11161 aggctgaagg cattggtgac tactggtaca acttgataga gacgaacggg cataatgtat
    11221 cgttgcgttc agatccgagg gaaatggatg cagacgaggt ctttgatgaa ttgagtcgtg
    11281 cagatatccg gattttagat gaaatctatt ctcggtatgg gcatatgaac cgatttgatc
    11341 tcgcaaatat gacgcattta gaaagcgttt gtccagagtg gcacgatcct ggcaattctc
    11401 gtaagcctat agacctgaaa gaaatgctga tcagtgaggg taaaagcgag gatgaggcta
    11461 atcgcataat tggcaaaatg gaagaatctc agaaacttaa ggaattttct ttgcaattat
    11521 catgacggat tatcagccat acaggaaagg aactgtgctt gccccaactg gaccatgcaa
    11581 tcatcttcat gtgatttgta atgatcctgt ttattacccc gttaacgatt gttattgtgt
    11641 tttagttgtt aatatttcta gtatcaagga tggtgtcccc cacgatccgt cttgcgtctt
    11701 gaattctggt gatcatcgct ttatcaagca tccaagttat gttgtttacg ctgaagctat
    11761 aatttggcga gtggataaca tggttagaaa gcagcgatcg ggtgagattt ctgttcatga
    11821 tgatatgcca gaagctacat tcaatagaat tctggacggt tttgatatct ct
//
LOCUS       DADLSA010000115        11863 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.115, whole genome shotgun sequence.
ACCESSION   DADLSA010000115 DADLSA010000000
VERSION     DADLSA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.115"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            252..699
                     /locus_tag="JN691_003081"
                     /pseudo
     CDS             252..699
                     /locus_tag="JN691_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134906.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            699..1691
                     /gene="lsrD"
                     /locus_tag="JN691_003082"
     CDS             699..1691
                     /gene="lsrD"
                     /locus_tag="JN691_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HAY7244463.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTATAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            1703..2725
                     /gene="lsrB"
                     /locus_tag="JN691_003083"
     CDS             1703..2725
                     /gene="lsrB"
                     /locus_tag="JN691_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HAY7244464.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYGERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            2752..3627
                     /gene="lsrF"
                     /locus_tag="JN691_003084"
     CDS             2752..3627
                     /gene="lsrF"
                     /locus_tag="JN691_003084"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HAY7244465.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            3651..3941
                     /gene="lsrG"
                     /locus_tag="JN691_003085"
     CDS             3651..3941
                     /gene="lsrG"
                     /locus_tag="JN691_003085"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HAY7244466.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            3980..4738
                     /gene="tam"
                     /locus_tag="JN691_003086"
     CDS             3980..4738
                     /gene="tam"
                     /locus_tag="JN691_003086"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HAY7244467.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(4742..5656)
                     /locus_tag="JN691_003087"
     CDS             complement(4742..5656)
                     /locus_tag="JN691_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family inner membrane protein"
                     /protein_id="HAY7244468.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(5863..7314)
                     /locus_tag="JN691_003088"
     CDS             complement(5863..7314)
                     /locus_tag="JN691_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAY7244469.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLVGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(7541..8959)
                     /locus_tag="JN691_003089"
     CDS             complement(7541..8959)
                     /locus_tag="JN691_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7244470.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(9098..9457)
                     /locus_tag="JN691_003090"
     CDS             complement(9098..9457)
                     /locus_tag="JN691_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HAY7244471.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(9457..10383)
                     /gene="glsB"
                     /locus_tag="JN691_003091"
     CDS             complement(9457..10383)
                     /gene="glsB"
                     /locus_tag="JN691_003091"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAY7244472.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(10447..11406)
                     /locus_tag="JN691_003092"
     CDS             complement(10447..11406)
                     /locus_tag="JN691_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY7244473.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAAIGAQAGAALKKCVLELGGSDPFIVLN
                     DADLELAVKAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDE
                     ENALGPMARFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTA
                     FREEMFGPVAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFI
                     NGYCASDARVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            11486..>11863
                     /gene="yneK"
                     /locus_tag="JN691_003093"
     CDS             11486..>11863
                     /gene="yneK"
                     /locus_tag="JN691_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY7244474.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINI"
BASE COUNT         3076 a         2973 c         2937 g         2877 t
ORIGIN      
        1 gacagacact gcgctcttag catggggcag ggaataacca ggctatttct ccatgcacag
       61 gccaattatg aaagaggaga ggcttaagtt cttgaaccct gaacgaataa cgactaccag
      121 aaaagcactt cgcatcactc agacatgatt taacataata tacattatgc gcagtaacat
      181 tgtagtagct aaattgagat gtccgtctct gactaaatag acatgtccgc gctacagtct
      241 caccacagcg attgctgctt ggcatttcag caattggttg gttgacgata attctggtgg
      301 catttatggc ctggctgcat ggcggcactg gcgggaattg tgtttgcttc gcagattggt
      361 tttatcccca accagaccgg taccgggctg gagatgaaag caattgcagc ctgcgtgctg
      421 ggcggcatta gtttgctcgg tggttccggt gcgatcatcg gtgcggtact cggcgcatgg
      481 ttcctgacgc agatcgatag cgtactggtg ctgttgcgca ttccggcatg gtggaatgat
      541 tttatcgcgg gtctggttct gctggcggtg ctggtgtttg atggacgcct gcgttgtgcg
      601 ctggaacgta atctacggcg gcaaaaatat gcccgcttta tgacgccacc gccatccgtt
      661 aaacccgctt cgtcaggtaa aaaacgggag gccgcataat gcgtattcgc tacggttggg
      721 aactggctct tgccgcactg ctcgttattg agattgtcgc atttggtgca attaacccgc
      781 gaatgttaga tctcaatatg ttgctgttca gcaccagtga ctttatctgc attggcattg
      841 tcgccctacc gctgacgatg gtgattgtca gtggcgggat cgatatttcg tttggttcga
      901 ccatcggcct ctgcgccatt gcattgggcg tactgtttca aagtggtgtg ccgatgccgc
      961 tggcgatact cctgacctta ctgctcggcg cattgtgcgg gctgatcaac gccggattaa
     1021 ttatctatac caaagttaac ccgctggtga ttacgcttgg cacgctgtat ctgtttgccg
     1081 gaagcgctct gctgctttcc ggtatggccg gagcgacggg gtacgaaggt attggtggat
     1141 tcccgatggc gtttacagat ttcgctaacc tggatgtgct gggactcccc gttccgctga
     1201 ttatcttcct gatatgtctc ctcgttttct ggctctggct gcataaaacc catgccggac
     1261 gtaatgtgtt tttgattggg caaagcccgc gcgtggcgct ttatagcgcg attccagtta
     1321 accgcacctt atgtgcgctc tatgccatga cggggctggc gtctgcggtc gccgctgtgc
     1381 tgctggtatc gtattttggt tcagcacgtt ccgatctcgg tgcgtcgttt ctgatgcccg
     1441 ccatcaccgc agtggtgctt ggcggggcca atatttatgg tggttccggt tccattatcg
     1501 gcaccgccac tgcggtttta ttagtgggat atttgcagca aggtttgcaa atggcaggag
     1561 tgccaaatca ggtgtccagc gccctttccg gtgcgctgct tatcgtcgtt gtcgtaggtc
     1621 gttccgttag cctgcatcgc caacaaatta aagagtggct ggcgcgtcgg gccaataacc
     1681 cattgccata aaggatatct tcatgacact tcatcgcttt aagaaaatcg ccttactgag
     1741 cgctcttggc attgccgcaa tctctatgaa tgtgcaggcc gcagagcgta ttgcatttat
     1801 tcccaaactg gttggcgtgg gattttttac cagcggtggc aacggcgcac aacaagcggg
     1861 taaagagctg agcgttgatg tgacctacga cgggccgaca gaacccagtg tttctggtca
     1921 ggtacagttg attaataact tcgtcaatca aggttataac gccattatcg tttctgcggt
     1981 ttcgcctgat ggcttgtgtc cggcactgaa acgcgccatg caacgtggtg tgagagtgct
     2041 gacctgggac tctgatacta aaccggagtg ccgctcttac tacattaatc agggaacgcc
     2101 cgcccagttg ggaggtatgt tggtggatat ggcggcgcgt caggtgaata aagacaaagc
     2161 caaagtcgcg tttttctact caagccccac cgttacggac caaaaccagt gggtgaaaga
     2221 agcgaaagcg aaaatcgcca aagagcatcc aggctgggaa attgtcacta cgcagtttgg
     2281 ctataacgat gccactaaat cgttacaaac cgcagaagga atattaaaag cgtatagcga
     2341 tctcgacgcc attatcgccc ccgatgccaa cgccctgccc gctgccgcac aagccgcaga
     2401 aaacctgaaa aatgacaaag tagcgattgt cggattcagt acgccaaatg tgatgcgccc
     2461 gtatggagag cgcggcacgg tgaaagaatt tggactgtgg gatgtggttc agcaaggcaa
     2521 aatttcagtg tatgtcgcgg atgcattatt gaaaaaagga tcaatgaaaa cgggcgacaa
     2581 gctggatatc cagggcgtag gtcaggttga agtctcgcca aacagcgttc agggctatga
     2641 ctacgaagcg gatggtaatg gcatcgtact gttaccggag cgcgtgatat tcaacaaaga
     2701 gaatatcggc aaatacgatt tctgatgtgc ataacttaac cggagtaagt tatggcagat
     2761 ttagacgata ttaaagatgg taaagatttt cgtaccgatc aaccgcaaca aaatatccct
     2821 tttaccctga aaggttgcgg tgcgctggat tggggaatgc agtcacgctt atcgtggata
     2881 tttaatccga aaacgggtaa aactgtgatg ctggcctttg accatggtta ttttcagggg
     2941 ccgactaccg gacttgaacg cattgatata aatatcgccc cgctgtttga acatgccgat
     3001 gtattaatgt gtacgcgcgg cattttgcgc agcgtagttc ccccggcgac caataagccg
     3061 gtggtactgc gggcgtcagg tgcgaactct attctggcgg aattaagtaa tgaagccgtg
     3121 gcgttatcga tggatgatgc cgtgcgcctg aacagttgcg cggtggcggc gcaggtttat
     3181 atcggcagcg aatatgaaca tcagtcgatc aaaaatatta ttcagctggt cgatgccgga
     3241 atgaaagtgg gaatgccgac catggccgtg actggcgtgg gcaaagatat ggtgcgcgat
     3301 cagcgttatt tctcgctcgc gactcgaatc gccgctgaaa tgggggcgca aattatcaaa
     3361 acctattatg tcgaaaaagg ttttgaacgg attgttgccg gatgtccggt acccattgtt
     3421 attgctggcg gtaaaaaatt accggagcgc gaggcgctgg aaatgtgctg gcaggctatc
     3481 gatcagggcg cttctggtgt ggatatgggg cgtaatattt tccagtctga ccatccggtg
     3541 gcgatgatga aagccgtaca ggcggtggtt caccataacg aaacggctga tcgggcatat
     3601 gaactctatc tgagtgaaaa acagtaactg cggatttaag gagaagaatt atgcacgtca
     3661 cactggttga aattaacgtt catgaagaca aggttgacga gtttatcgaa gtttttcgcc
     3721 agaaccacct gggctctgta caggaagaag gtaatttgcg cttcgatgtc ttacaggacc
     3781 cggaagtgaa ttcgcgcttt tatatttacg aagcctataa agatgaagac acagtggcat
     3841 tccataaaac cacgcctcac tacaaaacct gtgtcgcgaa actggaatct ttaatgaccg
     3901 ggccgcgtaa aaaacgtctg ttcaatgggt tgatgccgtg aggctacaat tggggtaacg
     3961 cgctgacggg agtaaaaaaa tgtctgactg gaacccctct ttatatctgc acttttccgc
     4021 tgaacgatcg cgtccggcgg tggagctgct tgccagagtg ccgctggaga atgtcgaata
     4081 tgttgccgat cttggctgtg gtccaggtaa cagcaccgcc ctgctgcatc aacgttggcc
     4141 tgcggccagg ataacaggca tcgactcgtc tccggcgatg attgctgaag cgcgcagtgc
     4201 tttgccagac tgccagttcg tggaagcaga catccgcaac tggcaaccgg aacaggcact
     4261 cgatctgatt tttgctaatg cctcactgca atggctgccc gaccactacg aattgtttcc
     4321 tcacttggtt tctttactta atccgcaggg cgtgctggca gtacagatgc cagataactg
     4381 gctggagccg acccatgtgc tcatgcgcga agttgcctgg gaacaaaact acccggatcg
     4441 cgggcgtgag ccgttggctg gcgttcatgc ttactacgat attttgagcg aagccggatg
     4501 tgaggtcgat atctggcgaa ccacttacta tcaccagatg ccgtcgcacc aggcgattat
     4561 cgattgggtg accgccactg gattacgtcc gtggttacag gatctgaccg agagcgaaca
     4621 gcagcttttt cttacgcgct accatcagat gctggaagag cagtatccac tgcaagagaa
     4681 cggacagata ctgctggcat ttccgcgtct gtttattgtt gcccgccgta cggagtaatt
     4741 atcacgtcag ctggtaatga cgatcgggaa gaatttttgc tggaatttct gcttcatcgt
     4801 tcatctgtaa caggtcaatt tcaatagcgt tgcagatggc atccagcggt aaatcattgt
     4861 tttcagtacc gaacggatct tccagttctt ccgccagaca atccagcgaa ataaaagtgt
     4921 aggaaatcag cacagagata aaaggcgtca tgtaatgcag gtccacgacc agcgcgaacg
     4981 gcagcatgat acaaaacaga taaacggtac gatgcaaaat cagggtgtag gcaaagggaa
     5041 ttggcgtgta ggcaatgcgc tcacagcctg ccagaaccgc tgaaatatca ttaagccgat
     5101 cgttgaggct aataaacagg atatctgaaa gctgtccatt gcggcgctga accgccaacc
     5161 attctcccat tattaacaag atacggttag ctggagagtt cgaagccagt acacgctgaa
     5221 gatcttcagt cttgagataa tgagccagca cttccgcctg tggctgtttg cgtaatgtca
     5281 tgcgtaaaca gtgggcgaag gcgatttgca gcctggcaaa ctcccttaca cttgccgaat
     5341 ccggcaatgt cgtttttacc tctcgcagta acgaccgtga ggcaatcatc aactgccccc
     5401 aaagttttcg cgcttcaacg taacgggcgt acccggcatt attacgaaaa ccaagaaaaa
     5461 tggcgatggc gacaccgaga atgctgaacg gtgcgagggt gaatttgatg cccagatgcg
     5521 tgtaccaggg cagcatgaat ataacagcaa tagaaaagag aaaattgagt agtaagcgcg
     5581 aggatatctt ggataatact gagccgtgcc agacaaaaat acggcgcagc cagtgttgtt
     5641 gtggacgaac aatcatggtt atcttcaggc gtggaaaagt cgccctatta aacgtgatta
     5701 cgatcacatt ctcaagacgc tacttacaaa ttacctacaa gcttacaaca ttaacaggcg
     5761 ctgcatgtga cacctgttaa tgataaaggc atataggatg ttgtaactaa tatagtgaaa
     5821 taagaaaccc ggtcgaaacc gggttcagaa gtaacggtgt tattagcaca acggacgtac
     5881 agcttcgcgc atcccttttt caagaatcgc atccaggtca ttagcaacct gctcgaccag
     5941 accaggcact tgcgtcaggt cctgctccca gtggtctttt tctgccaaca caatcgctac
     6001 cagttcctgg gtaccgataa cacggtcacg atgctggctc cagagttgct gataacgttc
     6061 cagccagtgt gcatcgtcct gtaccggata tgtttctccg ttacgctcac cacgatagaa
     6121 cgcaatcaat gccgctaatg cgaaagtaag gcgcgccgga agtgtgccgt ttgccttctg
     6181 ccctaccagc agctgcggca ggatgcgggt gcggaacttg gtcataccgt tgagcgcgat
     6241 agacagcagc tgatgcttaa tgtacgggtt acggaaacgc ccggtgactg cactggcgaa
     6301 agattccagt tcatcacgag gcaaatccag taccggaata atttcttcgt agatggcttt
     6361 ttcaacgaat gcgcaaattt cagcatcgtt catcgcttca cctacggtat ccagccctgc
     6421 ctggaacgcc accggcacca gcgcggtgtg cgcaccgttg aggattgcca ctttgcgttc
     6481 tttatacggt ttaatatcgt caacaatcag cacgttgagc ggatatttgt ccaggcgcag
     6541 ctcagtcgct aaggatttcg gcccctgaat cacaaacagg taaaagtgtt cagcggtgtc
     6601 gaggaaacca tcgtgataac ccagttcttc ttccagttta gccacttcat cgcgcggata
     6661 accggtaacg atacggtcca ccagcgtaga acagaagctg ttagcctgat ccagccattg
     6721 aataaatgct tctggcagtg cccactcttg cgcatagcgc agcaccagtt cacgcaacgc
     6781 gtcgccattg tagtcaatca actcacacgg aatgatgatc caacctttat ccagcgcacc
     6841 gttgaaatgg ctaaagcgtt cgaacagcag acgggtcagt tttgccggat agcttaccgc
     6901 tggcgcgtca tcgaatttat cgcccgcatg gtagctgata cccgcttcgg tggtgttaga
     6961 gaaaacaaag cgcatttccg ggttgtgcgc cagtttcagg aattcatcgt attcactgta
     7021 gacgctgatt tcacgattaa ccgagcgaat cagacgcgcg tcgctgaccg cttccccctt
     7081 ctcattcagg ccacggataa tggtggtgta cagaccatcc tgcgtgctca gtgacggcgg
     7141 gaatgaagtt tcaatcggac gaacaacgac cacgccagaa ttcagatcgg tgtgctcatt
     7201 caggagatcg atttgccagt caacaaaggc gcgcaggaag ttaccttcac caaactgaat
     7261 gatacgttct ggatactgtg caccgggaaa atcgcgacga tttagtgttt tcacaatggg
     7321 ttcccttctg attagtcata caacctgttt gaattggtgc gacaggttag caaactttaa
     7381 tacgccgaac ccctgttttg atcaactcct gatgattaat gagcagtttt atgagaaaag
     7441 tgtggcgcga atcatggttt aatcgaggaa atacgccttt tcctggatca taaagtggta
     7501 gaacacattg cattcaaatc gcgcggaatg aataaagatg tcagacaact tcctcaccgt
     7561 aacgcatagt gctggtacgg ttgcgcccat ctttcttcga gcgatacaga cacgtatcgg
     7621 cttcgaccat caatttatta aaatcatcgg tcagcgttcg gtacgatgcg cgaccactac
     7681 cgacgccaat acttaccgtg agataaagcg ttttttgttg ccaggtgaat ggttgcagtt
     7741 caacgccttt acggattttt tccgccatta gcagaccatc gacaggattc accgacggca
     7801 ctgcaacagc aaattcttcg ccgcccatgc gcgccaccag ccccttatca ccgacaatct
     7861 tctgaatatg ccgggcaaac acgcttaaca ctttatcgcc acattcatgt ccatagttat
     7921 cgttaatgct tttgaaatag tcgatatcaa gcagcatgac cgtcagatgt tgtgtctgtt
     7981 tcagcgatgg acttttcagc gcctcataaa gaccggaccg tgagtaaacc tgagtcagaa
     8041 aatcaaagtc ggctcgcagc gcaacttgct tcattagcaa attgatcgct gccacgctaa
     8101 aagaaaccat aattgggcat atcgccatcg tggcaatacc gagacgtgcg gagaacattt
     8161 ccggaatgga gaacggcgaa ccgaccgaga tatcaatcac cgaatttgcc accagtacga
     8221 tttccaccgc accggtgaca aaggtcaaca gacatgttac ctgcggcgta tagcgcactg
     8281 cacaccagat taatgcaggc agcgggaacg ccagactccc cgctccgcca atgactaccg
     8341 aggcaataac cgacacaata agcgcgatgg cgggcatcat ctgttctgct ttaaagcgcg
     8401 gcagcactcc aggaatagcc aacgtcagca tacaaggcac gatcaacacg cccgttgaga
     8461 attgctcact gaaccagtcc gcaagcaaag gccagaaatc cagactatca atactgaccg
     8521 aaccaatcgc cccgacaata gcgcataata atgcggcaat cagacagtaa ttaaatagcc
     8581 gtaaggcact tactggctca tacttatttt tccccagacg cttatcccgc acgaccagta
     8641 aggcgacggt aacaataaac atcatattgg agaaattgat aaccagtgaa accagtcccc
     8701 attcggtggt tatggcatca taaaccagca tcgccacata actgatcgca tagtaatgca
     8761 gtcgattaag ccagacatag cgggcaaaca cccccgccat tacgccattc agcggccaga
     8821 aaagagaaag ttcgtgaacc agccgtaact ctgcaccaat aaaatagaac agcgtggtta
     8881 gcgcaaaaat agcgatgcta ttacgtagca gatgaccttc ctggaacaaa cggaaagttg
     8941 aaatggggtg tacatgcatt aattatctgt ccatacaatt gcgggtggca attatctcaa
     9001 cgtaataagt aaattctcat aaaggatagc atgttcgcta acagcggcgg caaataaagg
     9061 taccctggga atgctatttt atcgttttcc gcaattctca tggctgattc atctgtacaa
     9121 ccagccagtg atagattacc gccacgatat aacgttgttg ttcttcgctt aacgaaacac
     9181 cctgcggaat gttatgccac ttagccagac aaccgcgaca gcaggtcgcg gtggcgtgct
     9241 gagcgataaa caccggatgc ccgcgcatgg gggtttgctt accgtcatta gccggtaatg
     9301 ccggagcaag acgtttagcg acaaaatccg ccgcatgttg ttcgatgact ggcgcgcctt
     9361 tctccaggca atactgacgc tctttcatgc ccagacgaaa gcgagagcga aattttgaac
     9421 gggataaacg cgcgaagagt ggatcgagag actgcattaa taaaccgaac gccctaactg
     9481 tttcgtcaat tgttcaagga cggcgatacc cgcaagcgag ttacctgcat catccagttc
     9541 cggactccag actgcgatgg ccatttcatg cggaacaatc gccacaatac cgccaccaac
     9601 gccagatttc gccggtagtc ccacccgcca ggcaaactcc cccgcgttct ggtacatacc
     9661 actggtcgcc atcagcgcgt taatttgccg cgcctgcatt ggcgtcacca caggttcatc
     9721 aatatgaata gctttcccct gattagccag aaagacaaac gtccgggcca gctctacaca
     9781 gctcattttc agagcgcagt aatgaaagta gttttgcaga acggttgtca cgtcatgatg
     9841 gaaattgcca aacgacttca tcagccaggc gatagccgca tttcgcgcgg aatgttcaaa
     9901 ttcggaacgc gctaccaccg tatcgtatga aatatcagac acaccgctta agccgcgcac
     9961 gacttccagc atacgttgcc gtggtgcgct tagtcgccct tgcaacatat cgcagaccac
    10021 cagcgcaccg gcattaatga acggattacg cggaataccc tgttccattt ccagttgcac
    10081 taaagaattg aacggtgatc cagacggatc tttgccgacg cgttgccaga tttcctcttc
    10141 ggagtaatga cgcatggcga caacgagact cagcactttg gaaatagact gaatggaaaa
    10201 acgttcttgc gcgtctccgg cctgaaaaag ctgtccgtca acggtacaga tagcaatccc
    10261 caatcgggaa ccgtctactg tagccagcgc cggaatataa tccgcgactt taccctgacc
    10321 aatgagcggc cgcacttgcc gcaagatgtt ttctaaaatt gcattatcca tggcgactgc
    10381 cactttctac tcctggaccg caggtctgaa aagacctgcg agtatatcag agctgaatat
    10441 gtcgcgtcag atccggtctt tccacactgt ctggatatta cagaattcgt gtaagccgaa
    10501 atgggaaagc tcacgaccaa agccactctt tttcacgcca ccaaaggcca ctcgcgcgtc
    10561 gctggcacaa taaccattga taaacacccc accgcattcc agacgtgccg ccatctgtct
    10621 ggcctgtgtt tcgtcggtgg taaaaatggt cgctgaaagg ccgaactcac tatcattagc
    10681 cagttccagc gcatgttctg catctttcgc aatggtgatt gcagcaacgg ggccaaacat
    10741 ttcttcccga aacgcggtca tttctggggt aacattcgcc agaaccgttg gcggatagta
    10801 gttacctgcc ccagccatct tttccccgcc cagtaacaaa cgcgcaccct gagccagggt
    10861 tttctccacc tgatgatgca gctcatcacg taaatcaaaa cgagccattg gtccgagagc
    10921 gttctcttca tcacggggat cgcccatttt caaggctgcc gcagctgaca caaaacgttc
    10981 ggtaaatgcc gaagcaattc cctcttcgat aataaagcgt tttgccgctg cacaaacctg
    11041 tccggtattc tgataacgtc cggctaccgc cgctttaacc gccagttcca gatcggcatc
    11101 gttaagcaca ataaatggat ccgaaccgcc cagttccagt acgcattttt tcagtgccgc
    11161 tccagcctgt gcgccaatag ccgctccagc acgaacactt ccggtcaccg tgacagcagc
    11221 aatgcgcgag tctttaatca tctgactgac accgtcgttg tcggcattca gccagccata
    11281 tacgccttgc gggattcccg catctttaaa cacttgggca atgagctgag cacagcccat
    11341 cacattcggc gcatgtttaa gtaagtagcc gttacctgca agaatgatgg gaacagcgcc
    11401 acgcatcacc tgcctttgat agttcttgca gctcaaatgc cctatatagt gatgttccga
    11461 tgacttatga ctatatgggg caaatatggt tacgccagta agcatcagca attacatatc
    11521 tcttcctgat gattttcccg ctcgtaatat tgcacctcaa gtaaaagaag ttttaaagga
    11581 ttttattgat gcactcagta ccataatatg tgatgaagag tggcgcacgt ctttaaacac
    11641 caactcagcc acaaaaaaga tatttaacaa ccttgataac ttatcatata ttcagaggac
    11701 ttcttttcgg ggtaacgaca cactatacaa tgaaaaggtt cagtttaaac tcacttatcc
    11761 cgtcaagaat ggaagacaca aagaaaatat tgaatttcag gtagtaataa atttaagccc
    11821 tatttattta gataattttc gccatgatgg agaaattaat att
//
LOCUS       DADLSA010000116        11756 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.116, whole genome shotgun sequence.
ACCESSION   DADLSA010000116 DADLSA010000000
VERSION     DADLSA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.116"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(137..1501)
                     /gene="tdcG"
                     /locus_tag="JN691_003094"
     CDS             complement(137..1501)
                     /gene="tdcG"
                     /locus_tag="JN691_003094"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY7244475.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRNKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1573..1962)
                     /locus_tag="JN691_003095"
     CDS             complement(1573..1962)
                     /locus_tag="JN691_003095"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAY7244476.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(1976..4270)
                     /gene="tdcE"
                     /locus_tag="JN691_003096"
     CDS             complement(1976..4270)
                     /gene="tdcE"
                     /locus_tag="JN691_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HAY7244477.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4304..5512)
                     /gene="tdcD"
                     /locus_tag="JN691_003097"
     CDS             complement(4304..5512)
                     /gene="tdcD"
                     /locus_tag="JN691_003097"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAY7244478.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5538..6869)
                     /gene="tdcC"
                     /locus_tag="JN691_003098"
     CDS             complement(5538..6869)
                     /gene="tdcC"
                     /locus_tag="JN691_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAY7244479.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(6891..7880)
                     /gene="tdcB"
                     /locus_tag="JN691_003099"
     CDS             complement(6891..7880)
                     /gene="tdcB"
                     /locus_tag="JN691_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAY7244480.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSLLIDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(7979..8917)
                     /gene="tdcA"
                     /locus_tag="JN691_003100"
     CDS             complement(7979..8917)
                     /gene="tdcA"
                     /locus_tag="JN691_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAY7244481.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            9107..9451
                     /gene="tdcR"
                     /locus_tag="JN691_003101"
     CDS             9107..9451
                     /gene="tdcR"
                     /locus_tag="JN691_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HAY7244482.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            9707..10247
                     /locus_tag="JN691_003102"
                     /pseudo
     CDS             9707..10247
                     /locus_tag="JN691_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10269..11435
                     /locus_tag="JN691_003103"
     CDS             10269..11435
                     /locus_tag="JN691_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
                     /protein_id="HAY7244483.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQEISTGNINVNCPLKDIYNEIKRLNVIFSCGTGDIVDLSSLDLRN
                     VDLDYYDFTDKHMANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKITPV
                     LISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELTKE
                     IYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYTMD
                     LNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         3335 a         2839 c         2549 g         3033 t
ORIGIN      
        1 aagactttaa aataacggag atggataaga attttctact aaattaatcg caagagaata
       61 ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca cccaaggatg
      121 aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg cgtgatgttt
      181 cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc actttatcga
      241 gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt actgctttca
      301 cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca accggatcgc
      361 aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc tgcgccggac
      421 tgccgcccag tagttcagtt aaccctgccg ccgccattga acaggccacg ccaatctccc
      481 cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc gcgccaatag
      541 cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga cggaacttat
      601 cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt gccgttacca
      661 cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg atccagtcga
      721 tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga cgcagcgcta
      781 cggcacgacg cggcacattg agcggaccag gcagcacgcc ttcagtgttc atcccgcgtt
      841 caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt tccgctttgt
      901 tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg ttgtaatcac
      961 acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt tcgacatcgt
     1021 gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag taataggttt
     1081 tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc tcatggcgag
     1141 gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca atatgcgcac
     1201 cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca gggatctcat
     1261 caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg acatccgtgg
     1321 catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata tggctcgtcg
     1381 cggttaataa gccgctactt tccagccgat caataaaact ttttccggca ttcattggcc
     1441 ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat gcactaatca
     1501 tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg cgaccgtccc
     1561 gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct tcacatcttt
     1621 cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat gctcatcgaa
     1681 gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga tgaacacggt
     1741 catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt tgacgttttc
     1801 gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct gcgggcaaac
     1861 cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa cataagggcc
     1921 gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc cggcgtcaga
     1981 gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc agtgcgttga
     2041 agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc gggtgctcga
     2101 tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga ccgccttcga
     2161 catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt ttacgtactg
     2221 gatcttcttt gcccagcgcc gcaggaacga ttgagaaagt gtacgagatc ccatctttgg
     2281 cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct ttgcggtcac
     2341 gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga ccgtccggcg
     2401 tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc gactgggtag
     2461 ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt tcaaccaggt
     2521 cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat tcaccgtcga
     2581 tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg gcatatttga
     2641 tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc atagtgcgat
     2701 agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg tcgtgcatgt
     2761 agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg aagtgatcga
     2821 ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct gttttcggcc
     2881 cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc agcgttttcg
     2941 ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt gggctgacgc
     3001 agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg tcattttcat
     3061 actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag gcaatcggta
     3121 attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata gtgtgcaggg
     3181 tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc cccatcccgc
     3241 cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat tccggtgtac
     3301 gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc tgtgcctgct
     3361 gctcattgag gacgccagct ttaaagtcgc gctcaatgta gatatccagg aacgatgccg
     3421 tgcgccccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga taagcgaagt
     3481 agagccactg caccgcttcc tgtgcattct gcgccgggcg agagatatca aagccatatt
     3541 tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc tcctcacgca
     3601 gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga tcggcaaatt
     3661 gcagttcgcg ttcacgcacc agataactga tgccatacag cgctacgcgg cgatagtcac
     3721 cgataatgcg cccacgacca tagccatctg gtaaaccggt cagcacgcca gatttacggc
     3781 agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt ttacgcagat
     3841 cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg aatgaacttt
     3901 taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca tccgtttgca
     3961 ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg gtaattgtgg
     4021 tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata cggatgcctt
     4081 ccattacttt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga aaagattcat
     4141 cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt tcgcttttcc
     4201 agtccgtacc tttaaagcca agccatgcgt cagcgtacag cttatcgctg gtatcaatat
     4261 ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca aattctgcgg
     4321 gcgcgttaac tttacctaaa tgaatggcat ccaaagcaat cattttttct tcgttagtcg
     4381 gaataacgac gcaaatgaca tgcgcatttt cactggaaac aattcgctcg ccaaaagagt
     4441 tagatcgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa tgttccatga
     4501 ccagacgacg aattaaactt gaattctctc ctattccgcc ggtgaatata attccatcca
     4561 gccgacgtaa tgaagctgcg tgtccggcaa tatgacgggc aattcggtga acaaaggttt
     4621 taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga acacgtaaat
     4681 ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg cgttccaggt
     4741 cacccaggct ctggttggtt tggctggcga cccaggacat cgcaccaaag tcgacatcgc
     4801 cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag gtgtcaacac
     4861 tctgaccgtt gcgaactgcg cagattgacg cgccattgcc aagatgcgcc acaaccaggc
     4921 cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca tagcggtgcg
     4981 acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc cacggcaggc
     5041 cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat accgccacct
     5101 gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattt gcgtaattat
     5161 gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacttcatcg gtaataatgg
     5221 cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag gccacgctgt
     5281 catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct tcgtagctgt
     5341 ggagagccag cggtgctggc tctcccccat ttacggataa gaatgcattt tccgagttaa
     5401 taccgtcggc aatacctgac attaatactt cacagtcgct ggcatcgagt acggaaaact
     5461 taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc atctcttttc
     5521 tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt cagcagacca
     5581 atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga cggcgcttta
     5641 cggatagcat acatcggcaa caggcacagc agggatgcga taatcggtgc gcccatggct
     5701 tcaatcaggt cgaggatgtt cgggttggcg taggcaacaa cccaggtgga gcccatgatg
     5761 aagatcatgc tgagagtatt cagtttaccc agcgacactt tggttttgtc acctttataa
     5821 ccgaacttca gaatcagacc attcaagcct tccagcgtcc ccagatagtg accgaagaaa
     5881 gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt aatcgcgaac
     5941 gttgttttcg taccggtcat ggacgcaaag tggttagcca gataagaaag cactggaata
     6001 ttctgcgctt tggcttccgc catgttggcc ggagacagag taaacaggca gctaaaggca
     6061 aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg ggaacattta
     6121 cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga aaccacaaag
     6181 gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc cagccagaca
     6241 gtaatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac ctggtcgata
     6301 actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct ggcgataaac
     6361 ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca gatgacgaaa
     6421 gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc aaatccgagc
     6481 tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat ccacagcagt
     6541 gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt accaaaatgc
     6601 tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca cagacgcgcc
     6661 agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag aagaatcggg
     6721 atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc gattgccgtg
     6781 ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg ttttgtctgg
     6841 ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa ttaagcgtca
     6901 acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga aataatactg
     6961 acggtttttc tgttttgaat atattggtct aatttaccgc ttaataatgc agcacatgcc
     7021 agagcgcctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat catactgttt
     7081 ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac gatttcgtaa
     7141 gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt agttcggtgc
     7201 gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc tgactgtacg
     7261 ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc agcaattaaa
     7321 ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc cataatttcc
     7381 agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg gataaaaata
     7441 cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa gttatcacca
     7501 tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt tggcgcacct
     7561 tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga aaccccttgc
     7621 gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc gatcagtaaa
     7681 cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat attttcaaac
     7741 ttcaggaata tttcaccttt gcaacgttca ctaaaatagt tggagcgagg catgcctgtt
     7801 ttataaattc gcccagccag tcgttgtttc gcttcaataa tatcatcaat agcaaccggc
     7861 agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg taggtaaccg
     7921 taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa acaaataact
     7981 aaccaacttc tattaattgc cttcgtctac acccattata agatgaatac tctttggcta
     8041 attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat accgcggcat
     8101 attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgatta gaaccaaaag
     8161 gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca agattataaa
     8221 ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt ctttgtaacg
     8281 tagtaagcag ttcgctgtag taccccatat tagtttgtgg caacacccac tgttcgttct
     8341 tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct accagcacaa
     8401 actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct gcacttaacg
     8461 taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa gacagttgcg
     8521 cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg atcatccctg
     8581 acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc accgcctcag
     8641 aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg gattcggaac
     8701 gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc cgcaccacta
     8761 attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc ggttgagtta
     8821 accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc tgaaagacta
     8881 ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt aattacctca
     8941 ttgacggcat gaagtgtatc aaaatgaaat gaacaggata tgtgcgacca ctcacaaatt
     9001 aactttcatt actttccagg gtatcgttat tacaattaaa cacctttaaa tatcaacaag
     9061 ttaaagcata aaaatcagca taaagcctga tttttatcaa aaaaaaatga cggggataac
     9121 catattttat ggcgataaca tcattcgtta tgtggttaat acaaaaaagg ggctgagacc
     9181 atatttcaag caattaccgg ataattacca ggcaaaattt gagttaaatt taatgagcaa
     9241 gttctcaaat tttattataa ataaaccatt ttcagcgata aatactgcgg cacgtcacat
     9301 tttttcacgc tatttgttgg agaacaaaca tttattttat caatatttta aaatttcgaa
     9361 tacaggtatt gatcatctcg aacaattgat taacgtcaac tttttctctt ctgacaggac
     9421 gtcattttgt gaatgcaatc gttttccata aattcttctc ccctcatagg cgacgaatag
     9481 cattttgtgt tgaggatcac aaaacgaata attgctggtc gccgcgataa ggtcagacga
     9541 agtcaacaag ggggattttc actgtgcttt acatcagcgt aggacacaga atgttgcttc
     9601 gtttactgta acgccgggta aatgagcgtt ttttgatagt gcgtaagtgt cgtgcgagga
     9661 aaataagcat ctattattgt ttgtcatgac gacaaggcag gcatcaatga aaggcttccc
     9721 aattgcacat atttttcacc cttcaatccc gccaatgcac gcagtggtta acaatcacaa
     9781 tagaaatatt gattattgga cggtaaaaag aaagtttgca gaaattgtct ccaccaatga
     9841 cgttaataaa atttacagta taagtaatga actgcggaga gtattatctg caattactgc
     9901 attgaatttc tatcaaggcg atgttccttc tgtcatgatc cgaatccaac cggaaaatat
     9961 gagtccattc atcatagata tttctacagg agaacatgat gattatatca tacaaacatt
    10021 agatgtaggc acttttgcac cttttggtga acaatgtact tgctcagccg tcaataaaaa
    10081 agagctggaa tgtattaagg agacgatttc taagtattgc gcaaagttca cccaaaaaga
    10141 agccatctta acacccccta ggcactttaa taaaacctgc attacttcag attgttggca
    10201 aattctcttt tttttcaccc gatcatttta ataatgattt ttattgatta tttataaagg
    10261 ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat ttagtccgtc
    10321 gaaaaatgaa tgaccttcct gaaagcccca taggaaataa cctcgaagca ctagcaccag
    10381 gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac gcgttacaac
    10441 caagtggaaa atgcattatc actggtgatt ttcaaaaaga attagcgtac ttacaaaatg
    10501 taattcttta taatgactcg tctcttcgtc tggatttttt aggttataac gcccaaatta
    10561 ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa aaccaggaaa
    10621 tatctacagg caatatcaat gttaattgcc cattaaaaga tatttacaat gaaatcaaga
    10681 ggttgaacgt aatctttagt tgtgggactg gagatatagt tgatctatcc tctctggact
    10741 tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct aatactattc
    10801 taaatcattt taaattggat tcaacaaatt ttactaatgc caacatgttt caggttaatt
    10861 ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa ataacgcctg
    10921 ttttaataag cattagcgat atgtattctg aagaaaaaat caagtttgtc gaaagttgtt
    10981 taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt gcaattataa
    11041 aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca aaagaaatat
    11101 ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca gagatgaaag
    11161 aattctcatc aaaaaaaata tatgattaca tcgatacaat cattgaagat tatgaaaata
    11221 tcaaagaaaa tgcttatctg gtcgtccccc aaattaatta tactatggat ttaaacatag
    11281 aagactctag ctcagaagag ttaataccct cgagaaagac gaaaattctc cggacaatgg
    11341 ctttgaggtc ggggaatata acacatatga agcatataac tcagagaatc aatattttac
    11401 cagagaggac tatacgtata attacgacct tttaaatgca atatagactt aataacttct
    11461 tacaaagtct atcattaata ccgtagatat tcatcttacc ctgtgcacca aaagcaaaaa
    11521 cttatcgacg cgggtttgaa actgatggca accgcaaaaa tgcctgatgc gctacgctta
    11581 tcaggcctac gccatctctg caatatattg aatttgcgtg cttttgtagg caggataagg
    11641 cgttcatgca gcatccggca tgaataaagc gcactcaaca atctgaaacc cgccggaacg
    11701 gtttattacg tacatcaggt gaaactgacc gataagccgc tttcttttgg gtatag
//
LOCUS       DADLSA010000117        11748 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.117, whole genome shotgun sequence.
ACCESSION   DADLSA010000117 DADLSA010000000
VERSION     DADLSA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.117"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..1362)
                     /gene="cusC"
                     /locus_tag="JN691_003104"
     CDS             complement(<1..1362)
                     /gene="cusC"
                     /locus_tag="JN691_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HAY7244484.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYLNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEVERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISNASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTAL"
     gene            1519..2202
                     /gene="cusR"
                     /locus_tag="JN691_003105"
     CDS             1519..2202
                     /gene="cusR"
                     /locus_tag="JN691_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HAY7244485.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2192..3634
                     /gene="cusS"
                     /locus_tag="JN691_003106"
     CDS             2192..3634
                     /gene="cusS"
                     /locus_tag="JN691_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HAY7244486.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GALDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKCEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            3784..6021
                     /gene="nrfB"
                     /locus_tag="JN691_003107"
                     /pseudo
     CDS             3784..6021
                     /gene="nrfB"
                     /locus_tag="JN691_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            6008..8980
                     /gene="nfrA"
                     /locus_tag="JN691_003108"
                     /pseudo
     CDS             6008..8980
                     /gene="nfrA"
                     /locus_tag="JN691_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            8981..9871
                     /locus_tag="JN691_003109"
     CDS             8981..9871
                     /locus_tag="JN691_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HAY7244487.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            10054..10815
                     /locus_tag="JN691_003110"
     CDS             10054..10815
                     /locus_tag="JN691_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7244488.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPRLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(10861..10937)
                     /locus_tag="JN691_003111"
     tRNA            complement(10861..10937)
                     /locus_tag="JN691_003111"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10901..10903),aa:Arg,seq:tct)
     gene            11180..>11748
                     /gene="fimZ"
                     /locus_tag="JN691_003112"
     CDS             11180..>11748
                     /gene="fimZ"
                     /locus_tag="JN691_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HAY7244489.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYG"
BASE COUNT         2901 a         3021 c         2999 g         2827 t
ORIGIN      
        1 taaggcggta tacaaataaa tttcgttaac ctgacgggca taattcaggt caagtaaagt
       61 ttgtcgggtt gcaaataaag aacgctcggc atccagcact tccagataac ttaccgcgcc
      121 gtgctgatat aatgcccgcg cccgttgcag ggtaatttgc agcgacgcca gataacgctg
      181 ctgggcgctg atttgatcgt ctaggctttg acgtaatgcc agcgcatctg ccacctcttt
      241 aaaggcgttc tggattttct gttcataatt caccaccgac tgctgctggc gaatttcggc
      301 gatatccaga ttggcctggt tgcgtccggc attaaaaatg gggatctcaa ttttaggaat
      361 aaaattccac atcccgctgc tagcgttaaa taatgatgac agatcgctgc tggcgtttga
      421 tattccgctg gtcaggctga tagacgggaa aaatgccgca cgcgcagcac caatattagc
      481 attagccgcc attaacgcgt gttcagcttc cataatatcc gggcgctgca ataagatttg
      541 cgacgacaag cccgccggta atttaacgct ttgcaggctg ccgctgttta ctgtctgcgc
      601 ttgcggtagc ttgccgtagc ttcccaataa cagttgcaat gcattattcg cctgcgccag
      661 ttccccctga cgtttagcga tgtcactgcg ggtactttct atcaccccgc gagcctgttc
      721 cagcgccaga acattgctgc taccggtcaa cagttgtttt tcgacaaacg catatgactg
      781 ctgataatta cgcagcgttt cttcggctat ttgcaattgc gcatacgcca gctgctgatt
      841 gaaatagctt tgcgcgacat tagaaaccag caaaatatgc accgcgcgct gggcttcctc
      901 agtggctaaa taattttggc gctcgacttc gctcatgttc tttaagcgac cgaaaaaatc
      961 gagatcaaag ctggcgttaa ggccagtcga gaactcccgc gtcgtggctg aatcgccttt
     1021 aagattgccg ctccagctgc cgctgccctc gccattgagc tgtgggtagc ggtcggcatc
     1081 ggtcagacga tattgcgccc gtgcctcctg cactttcagc gtcgccatgc gcaaatcccg
     1141 gttattcacc agcgcctcgc taatcagcgt cttcacctga ttatcaacaa aaaaggtgcg
     1201 ccagcccgcg ttcaggtagt tatccgctgc gttaaccagg ccgttctggc tgagtgagaa
     1261 ctgctgcggt acgggcattg ccggacgctg ataatccggt gccagtgaac aaccagttag
     1321 cgcaagggcc acacaaaatg gcagaagttt acaaggagac ataggctcat aatttctggt
     1381 gattttatgt cgccaacttt actcgccagg ctctgatttt ccggtgacag gaaaatgaca
     1441 aaattgtcat tttgccaata agcgattgcc atctgatccc gctactctag aattgcccgg
     1501 gcaacatgcg gaggaaatat gaaactgttg attgtcgaag atgaaaagaa aaccggagaa
     1561 tacttgacca aagggttaac cgaagccggt tttgtggtcg atttggccga caacgggctg
     1621 aatggctacc atctggcgat gaccggtgat tatgacctga taatcctcga tattatgctg
     1681 ccagacgtga acggctggga tatcgtgcgc atgttgcgct ccgccaataa agggatgccg
     1741 attctgttgc ttaccgcgct tggcaccatt gaacatcgcg tcaaggggct ggagttgggg
     1801 gcagatgact acctggtgaa accattcgct tttgctgaac tgctggcgcg ggtgcgcact
     1861 ctcctgcggc gcggggcggc ggtgattatc gaaagtcagt ttcaggttgc cgatctgatg
     1921 gtcgatctcg tcagccgcaa agtcacccgc agcggcacgc gcatcactct gaccagtaaa
     1981 gaatttactc tgctggagtt ttttctccgc catcagggcg aagtgctgcc ccgctcgctt
     2041 atcgcctcgc aggtatggga catgaatttc gacagcgaca ctaacgccat tgatgtggcg
     2101 gtgaagcggc tacgcggcaa aatcgacaac gactttgagc cgaagctgat tcaaaccgtg
     2161 cgcggcgtgg gttacatgct tgaggtgccg gatggtcagt aagccatttc agcgcccgtt
     2221 ttcgctggca acccgcctga ccttttttat cagcctggcc accatcgcgg cgtttttcgc
     2281 ctttgcatgg atcatgatcc actcagtaaa agtgcatttt gccgagcagg acattaatga
     2341 tttaaaagag attagcgcca cccttgaacg ggtactaaat caccctgacg aaacgcaagc
     2401 ccgacgctta atgacgctgg aagatatcgt cagtggttat tccaacgtgt tgatttccct
     2461 ggcagatagc cacggcaaaa cggtgtatca ctcccccggt gcgcttgata tccgcgagtt
     2521 tgcacgtgat gccatacccg ataaagacgc gcggggtggc gaagtgtatc tcctttccgg
     2581 cccgacgatt atgatgccag gccacggtca cgggcatatg gaacacagca actggcggat
     2641 gattaacttg ccggttggcc cgttggtgga cggcaaaccg atttatacgc tctacatcgc
     2701 gctgtcgatc gattttcatc ttcattacat aaatgatttg atgaataaac ttattatgac
     2761 cgcatcggta atcagcatcc tgatcgtctt tatcgtgctg ttggcggtac ataaaggtca
     2821 cgcgccgatc cgcagcgtca gccgtcaaat ccagaatatc acctcgaaag atctcgacgt
     2881 tcgccttgac ccgcagaccg tgccgattga gctggaacag ctggtactgt cgttcaacca
     2941 tatgatcgag cgtattgagg atgtctttac tcgccagtcc aatttctcag cggatatcgc
     3001 ccacgaaatt cgcacaccaa ttactaatct cattacgcaa acggaaatcg ccctcagcca
     3061 gtcgcgcagc cagaaggagc tggaagatgt gctctactct aatctcgaag agctgacgcg
     3121 aatggcgaaa atggtcagcg atatgctgtt tctcgctcag gccgataaca accagctaat
     3181 ccccgaaaag aaaatgctca acctggcgga tgaagtcggc aaagtgttcg attttttcga
     3241 ggcgctagcg gaagatcgcg gcgtggagtt gcggtttgtt ggcgacaaac gtcaggttgc
     3301 gggcgatccg ttgatgctgc gtcgggcgct aagcaacctg ctttctaatg ccctgcgtta
     3361 tacgccaacc agagagacaa ttgtagtgcg ctgtcagacg gtcgatcacc aggtgcaagt
     3421 ttccgtcgaa aaccccggta cgcccattgc gcccgagcac ttaccgcgat tgtttgaccg
     3481 tttctatcgc gttgacccct cccgccagcg aaaatgtgaa ggtagcggta ttgggctggc
     3541 gatagtgaaa tcgattgttg tcgcgcataa aggcacggtt gcggtaacgt cagatgcgcg
     3601 ggggacaagg tttgttatca cattacccgc ttaattattc agcaaacggc aacttttata
     3661 accagtgtaa aaataacgtg ccgcaatagt catacctatt aatggtaaaa agctgtcaca
     3721 attcataaaa aaccttaata tacgccaccc taaacataac cagcgttaat gtaaggtttt
     3781 tgtgtggact ggcttcttga tgtttttgct acctggctct acggcttaaa agtaatcgcg
     3841 ataacgttag cggtcatcat gttcatcagc gggctggacg atttttttat tgatgtcgtc
     3901 tactgggtac gccgcattaa acgcaagttg agtgtttatc gccgctaccc gcgaatgagt
     3961 taccgcgaac tgtataaacc agatgaaaaa ccgttagcga ttatggttcc ggcgtggaat
     4021 gaaacgggcg tcatcggcaa tatggccgag ctggcggcga ccacgctcga ctacgaaaac
     4081 tatcatatct ttgttggcac ctaccccaac gaccccgata ctcaacgtga tgttgacgaa
     4141 gtgtgcgctc gctttccgaa cgtgcataag gtagtctgcg cgcgtcctgg tcccaccagc
     4201 aaagccgact gtctgaacaa cgtgctggac gccatcaccc agtttgaacg tagcgccaat
     4261 ttcgcttttg ctggttttat tctgcatgac gccgaagatg tgatttcacc gatggaattg
     4321 cgtctgttca actatctggt cgagcgtaaa gatctgattc agatcccagt gtatccgttt
     4381 gaacgcgaat ggacgcactt caccagcatg acttacattg atgagttttc agaactgcat
     4441 ggcaaagatg ttccggtgcg tgaagccctc gccggacaag tgcccagcgc aggcgtcggc
     4501 acctgtttca gccgccgcgc cgtgaccgca ctgttagctg acggtaacgg tattgctttc
     4561 gacgtgcaga gtcttactga agattacgac attggcttcc gcctgaaaga aaaaggtatg
     4621 acggaaattt ttgtccgttt tccggtggtg gacgaagcca aagaacgcga gcagcgtaaa
     4681 tttttacagc acgcacggac gtcaaacatg atctgcgtgc gcgaatattt ccccgatacc
     4741 ttttcgactg cggttcgaca aaaatctcgc tggatcatcg gcattgtttt ccaaggcttt
     4801 aaaacccata aatggacctc cagcctgacg ctgaactact ttctctggcg cgaccgcaaa
     4861 ggggcaatca gtaactttgt cagcttcctc gcgatgctgg tgatgatcca gcttttgctg
     4921 ttgctggcgt atgaaagttt gtggcccgat gcctggcatt tcctttctat ttttagcggc
     4981 agcgcatggt taatgaccct gctgtggcta aactttggct tgatggttaa ccgcatcgtg
     5041 cagcgagtga ttttcgtcac tggctactac ggcctcacgc agggcctgct ttccgtcctg
     5101 cgtcttttct ggggcaacct gattaacttt atggctaact ggcgcgtgct gaaacaggtt
     5161 cttcagcacg gcgatccgcg tcgcgtggcg tgggataaaa caacgcatga cttccccagc
     5221 gtcactggcg atacccgctc gttgcgcccg ttaggtcaaa ttctgctgga aaatcaggtc
     5281 atcactgaag aacaactcga tacagcactg cgtaatcgcg tcgaaggtct acgcctgggc
     5341 ggttcaatgc tgatgcaggg gctgattagc gccgagcagc tggcacaggc gctggcagag
     5401 caaaacggcg tggcgtgaga atccatcgat gcctggcaga tcccgtcctc gctgattgcc
     5461 gaaatgccag cctccgtagc gctgcattat gcagtactgc cgctgcgtct ggaaaatgat
     5521 gagttaattg tcggcagtga agatggtatt gacccggttt cgctggcggc cctgacgcgt
     5581 aaagtcggac gcaaagtgcg ttacgtcatt gttctgcggg gacaaattgt cacagggtta
     5641 cgccactggt atgcacgccg acgcggtcac gatccgcggg caatgttgta caatgcggtt
     5701 cagcatcagt ggctcacgga acagcagacc ggtgaaatct ggcggcaata tgtgccgcat
     5761 cagttcctgt tcgccgaaat actgaccacg ctcggtcata ttaatcgttc agcaattaac
     5821 gtgttgttat tgcgccatga acgcagttct ctgccgctcg gcaagttttt ggtcaccgaa
     5881 ggcgttatca gccaggaaac gttggatcgc gtcctgacaa ttcaacgcga attacaagtt
     5941 tcgatgcaat cactattact caaagcaggt ttaaacacag aacaggttgc gcaactggag
     6001 tccgaaaatg aaggagaata accttaatcg cgtcatcgga tggtctggtt tactgctgac
     6061 gtctttattg agtaccagcg cactcgcaga caatatcggc accagcgcag aagagctggg
     6121 gctgagcgat tatcgccatt ttgttattta tccccgtctc gataaggcgc tgaaggcaca
     6181 gaaaaataac gacgaagcaa ccgccatccg cgaatttgaa tatatacacc agcaggtgcc
     6241 ggataatatt ccgctgactt tataccttgc ggaagcctat cgtcattttg gtcatgatga
     6301 ccgggcgcgg ctgttgcttg aggatcaact gaaacgtcac ccaggagatg cccgacttga
     6361 gcgcagtctg gcggctattc cagttgaagt gaaaagcgtt acgactgttg aagaactgct
     6421 tgcccagcaa aaagcgggcg acgctgcgcc gaccctgcgt tgtcgcagtg aagtcgggca
     6481 gaatgccctg cggctggcac aattacctgt cgccagagcg caactgaacg atgcgacgtt
     6541 tgctgcatcg ccggaaggaa aaacgctgcg aaccgatctg ctgcaacggg caatctacct
     6601 gaaacaatgg tcccaggcag atacgctata caatgaagca cgccagcaga acacattaag
     6661 cgcggcagaa cgccgtcagt ggtttgacgt gcttcttgcc gggcagctgg acgatcggat
     6721 cctggcactg caatcacagg ggatcttcac cgatcctcag tcatatatta cttacgcgac
     6781 cgcgctggct tatcgtggcg aaaaagcacg cctccagcat tatctcattg aaaataagcc
     6841 gctgtttacc acgggcgcac aagagaaaag ttggctctat ctgttatcta aatacagcgc
     6901 caaccccgtt caggcattgg cgaattatac ggtgcagttt gccgataacc gccagtatgt
     6961 tgttggcgcg acgctaccgg tgctgttaaa agaaggtcag tacgacgcag cgcaaaaact
     7021 gctcgccacc ctccccgcca atgaaatgct tgaggagcgt tatgctgtca gcgtggcgac
     7081 ccgtaacaag gctgaatctc tgcgtctggc acgattgctg tatcagcaag aaccgacaaa
     7141 tcttccccgc ctggatcaac tgacctggca actgatgcag aacgagcagt cacgcgaagc
     7201 tgccgattta ttgctgcaac gctatccttt ccagggcgat gcgcgtgtca gccagacttt
     7261 aatggcgcga ctggcgtctc tgctggaaag tcatccttac ctagcaacgc cggcgaaggt
     7321 ggcgatttta tcgaaaccct taccgctggc ggagcaacgt cagtggcaaa gtcagttgcc
     7381 gggtattgca gataattgcc cggcaatagt tcgcttgctg ggcgatatgt cgccttccta
     7441 cgatgctgcc gcctggaacc gtctggcaaa gtgttatcgg gacacgctac ccggtgtggc
     7501 gttgtatgca tggcttcagg ccgaacaacg acaaccgagc gcctggcaac atcgtgcggt
     7561 agcctatcag gcgtatcagg ttgaggacta cgccaccgca ctggcggcct ggcagaaaat
     7621 cagtcttcac gacatgagca atgaggatct gctcgccgcc gccaacaccg ctcaggcagc
     7681 gggaaatggc gcggctcgcg atcgctggct tcaacaggca gaacaacgtg ggctgggaaa
     7741 caatgctcaa tactggtggc tgcatgcgca acgttatatc ccagctcagc cagaactcgc
     7801 actgaacgat ctcacgcgct caatcaatat tgccccttct gccaacgctt acgttgcgcg
     7861 ggcgacaatt tatcgccaac gtcataatgt tccggccgcg gtgagtgatt tgcgcgccgc
     7921 gctggaactg gaaccgaata atagcaacac ccaggcagcg cttggttacg ccttgtggga
     7981 tagcggtgat atcgcacagt cgcgggaaat gctcgaacag gcgcataaag ggcttccgga
     8041 cgatccggca ctgatccgac aactggccta cgtgaaccag cgtctggatg acatgcctgc
     8101 gacgcagcac tacgcccggc tggtgattga tgacattgat aatcaggcgc tgataacccc
     8161 actgacccca gagcaaaatc agcaacgctt caatttccgc tgtttgcatg aggaggtcgg
     8221 tcgccgctgg acgttcagtt tcgattcttc catcggcttg cgttccggcg caatgagtac
     8281 cgctaacaat aatgtcggcg gtgcagcgcc tgggaaaagc tatcgtagct acggacaact
     8341 ggaagccgag taccgcatcg gacgcaatat gctgctggaa ggcgacctgc tctcagttta
     8401 tagccgcgtc tttgccgata ccggagaaaa cggggtgatg atgccggtga aaaatccgat
     8461 gtccggcacc ggtctgcgct ggaagccgct gcgcgatcag atctttttcc tcgccgtcga
     8521 acagcagttg ccgctgaacg ggcaaaatgg cgcatccgat accatgctgc gcgccagcgc
     8581 ctcattcttt aatggcggca aatacagcga cgaatggcac ccgaacggtt caggctggtt
     8641 tgcccaaaac ctgtacctcg atgcggcgca atatatccgc caggatattc aggcgtggac
     8701 ggcagattat cgcgtcagct ggcatcagaa ggtagctaac ggacagacta ttgagcctta
     8761 cgctcacgtt caggacaacg gctatcgtga taaaggcact cagggcgcgc agcttggcgg
     8821 tgtcggggtc cgctggaata tttggaccgg cgagacgcac tacgacgcct agccgcacaa
     8881 agtcagtctc ggcgtcgagt atcaacatac ctttaaggcg attaatcaac gtaacggaga
     8941 gcgcaacaac gcgtttctca ccattggagt acactggtaa atgcgtaagt tcattttcgt
     9001 attgctgaca ctgcttttgg tcagcccttt ttcctttgcg atgaaaggta ttatctggca
     9061 accacaaaac cgagatagtc aggttaccga tacccagtgg caggggctga tgagtcagtt
     9121 acgttttcaa ggcttcgata ccctggtttt gcaatggacc cgttacggcg atgcatttac
     9181 ccagccagaa cagcgcacgt tattgtttaa gcgtgccgca gctgcgcaac agactggcct
     9241 gaagctgatt gtcgggctga acgccgatcc ggaatttttt atgcaccaga aacagtcgtc
     9301 tgcagcgctg gaaagctatc ttaatcgcct gctggctgcc gatctccagc aagccagatt
     9361 atggagcgcc gcgcctggca taacgccgga tggctggtac atcagcgcgg aaattgacga
     9421 cctgaactgg cgcagcgaag ccgcccgtca acctttgcta acatggttaa acaacgcgca
     9481 gcggctgatt agcgatgttt cagcaaaacc ggtttatatc agtagttttt tcgccggaaa
     9541 catgtcgccc gatggctatc gccaactgct ggaacaagtt aaagcaaccg gagttaatgt
     9601 ctgggtacag gatggcagcg gcgtggataa acttaccgct gaacagcgtg aacgttatct
     9661 acaggccagc gccgattgcc aaagctccgc cccagcaagc ggcattgttt atgaactgtt
     9721 tgtcgccggc aaaggcaaaa cctttacagc gaaaccgaaa ccggacgcag aaattgcctc
     9781 gctgttagcg aaacgttcct cttgcggtaa agacatgctc tatttctctc tgcgctattt
     9841 gcccgtcgcg cacggcattc tcgagtatta aatcccctcc aggtaagtcg ggtacgacct
     9901 ggcttacctc tttcgctctc gtcagaattc catcaagatt aattgctaaa aagcatgcta
     9961 aagaaagaag atcgttatcg gcgaacgggc cgatcactca tgcaggaagc atagatcctt
    10021 tgagcggagt ttctgatatc tgaggagtgc gaaatgcaat tgagcagcag tgaaccttgc
    10081 gtggtgatcc tgaccgaaaa agaggtagag gtaagcgtca ataaccatgc tacgtttacc
    10141 cttccgaaaa actacctggc cgccttcgcg tgcaacaata acgtcattga actctcaacg
    10201 ttaaatcacg tattaatcac ccacatcaac cgtaacatca tcaacgatta tctgttgttt
    10261 ttaaataaga aattaacctg tgtaaagccc tggtcgcggc tggcaacccc ggttatcgct
    10321 tgtcatagcc gtacaccgga agtgttccgg ctagccgcca accacagcaa gcagcaaccc
    10381 agcaaaccct gcgaagcgga gttgacgcgc gcattacttt ttaccgtatt gtctaacttt
    10441 cttgagcaat cgcggtttat tgccctactg atgtatatct tacgcagcag cgtccgcgac
    10501 agcgtctgcc gcattattca aagcgatatt cagcattact ggaatctgcg aattgtcgcc
    10561 agttcgctat gtttaagccc caggctgctc aaaaagaaat taaaaaacga aaataccagc
    10621 tatagccaga ttgtcacaga gtgtcgtatg cgttacgccg tacagatgtt attgatggat
    10681 aacaaaaata tcactcaggt ggcgcaatta tgtggctata gcagcacgtc gtactttatc
    10741 tctgttttta aggcgtttta cagcctgaca ccgttgaatt atctcgccaa acagcgacaa
    10801 aaagtgatgt ggtgaagggc aaagcggaaa cggataagac gggcataaat gaagaagaaa
    10861 tggcgcgccc tgcaggattc gaacctgcgg cccacgactt agaaggtcgt tgctctatcc
    10921 aactgagcta agggcgcgtt gataccgcaa tgcggtgtaa tcgcgtgaat tatacggtca
    10981 acccttgctg agtcaatggc ttttgatctg gttgctgaac aagtgaacga ccgcgtctga
    11041 ttttctgatt tatttcgcta tagcggcaaa caaacgcaca ccgctgcgcg tctgaatcaa
    11101 gaaaacccgt attttcatgt atcaaagtac aatttaccgc cctaacggaa aattgtccgc
    11161 tcctatgaga ctggtaacta tgaaaccaac gtcggtgatc attatggata ctcatcctat
    11221 catcagaatg tctattgaag ttctgttgca aaaaaacagt gaattgcaga ttgtcctgaa
    11281 aacggatgat taccgcataa ccatcgatta tctccgaacc cgtcctgttg atttaatcat
    11341 tatggatata gacttgcccg gaacagacgg ttttaccttc ctgaaaagga tcaaacaaat
    11401 ccagagcaca gtgaaagtgt tatttttatc atcgaaatca gaatgctttt atgctggcag
    11461 agcgatacaa gctggtgcta acggttttgt cagtaaatgc aatgatcaga atgatatttt
    11521 tcatgccgtt cagatgatcc tctccggata cacgtttttt cccagcgaaa cgcttaacta
    11581 tataaaaagc aataaatgta gtacgaatag ttcaacggtc actgtgctat ctaatcgtga
    11641 agtgaccata ttacgttatc tggttagcgg attatctaat aaagaaattg ccgataagtt
    11701 attgcttagc aataaaacag ttagtgcgca taaatctaat atttatgg
//
LOCUS       DADLSA010000118        11724 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.118, whole genome shotgun sequence.
ACCESSION   DADLSA010000118 DADLSA010000000
VERSION     DADLSA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.118"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            1..2264
                     /locus_tag="JN691_003113"
                     /pseudo
     CDS             1..2264
                     /locus_tag="JN691_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            2265..3914
                     /locus_tag="JN691_003114"
                     /pseudo
     CDS             2265..3914
                     /locus_tag="JN691_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
     gene            3919..4695
                     /locus_tag="JN691_003115"
     CDS             3919..4695
                     /locus_tag="JN691_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HAY7244490.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4970..7819
                     /gene="rcsC"
                     /locus_tag="JN691_003116"
     CDS             4970..7819
                     /gene="rcsC"
                     /locus_tag="JN691_003116"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAY7244491.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEVQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="JN691_003117"
     CDS             complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="JN691_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAY7244492.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="JN691_003118"
     CDS             complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="JN691_003118"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAY7244493.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTLRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESDVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2842 a         2839 c         3156 g         2887 t
ORIGIN      
        1 ttaacctcgt cgctgaaagg taaacctgtt tctgcgcagc taacggtagg cgtggtcgat
       61 gaaatgatct acgcgctgca accagaaatc gcgccgaata tcggcaaatt tttctatccg
      121 ctggggcgta acaatgtgcg taccagctcc agtctgtcgt ttatcagcta cgaccaggcg
      181 ctctccagcg agccggttgc gcctggcgcg actaaccgca gcgagcggcg agtaaaaatg
      241 cttgaacgtc cacggcgtga agaggtggat accgcggcat ggatgccgtc actcacaacc
      301 gataaacaag gcaaagcgta tttcacgttc ctgatgcctg attcgttaac ccgctggcgt
      361 atcaccgcgc gtgggatgaa cggcgacggg ctggtcgggc aggggcgtgc ttatctgcgt
      421 tcggaaaaaa atctctacat gaagtggagt atgccaacgg tgtatcgcgt gggcgataaa
      481 ccggcggcag gactgtttat cttcagtcag caggataacg aaccggtggc gctggtgact
      541 aaatttgcag gcgctgagat gcgccagacg ttgacgctgc acaaaggggc gaattatatt
      601 tcgctgacgc agaatattca gcaatctggc ttgttaagtg cagaactgca acaaaatggg
      661 caagtgcagg acagcattag cacaaaactg tcttttgtgg acaacagctg gcccgttgaa
      721 cagcagaaaa atgtcatgct cggcggtggc gagaacgcgc tgatgttgcc cgagcaggcg
      781 agtaatatcc ggctacaaag tagtgaaacg ccgcaggaga ttttccgcaa caatcttgat
      841 gcgttagtcg atgaaccgtg gggtggggtg atcaacaccg gtagccgtct gatcccgctc
      901 agtctcgcct ggcgttcgct tgccgatcat caaagtgccg ccgctaacga cattcgtcag
      961 atgattcagg ataaccgtct gcggctgatg caactggcgg ggcccggagc gcgctttacc
     1021 tggtggggtg aagatggcaa tggtgacgcc ttccttacgg catgggcatg gtacgccgac
     1081 tggcaggcca gccaggcgct cggcgtaacg caacaaccgg aatactggca gcatatgctc
     1141 gacagttatg ccgagcaggc gggtaacatg ccgttattgc atcgggcgct ggtgctggcg
     1201 tgggcacagg agatgaatct gccgtgcaaa acgttgttga aagggttgga tgaagctatc
     1261 gcccggcgcg gaactaaaga tgaagatttc tctgaggaag acatccgcga tatcaatgac
     1321 agcctgatcc tcgatacacc ggaatctcca ctggcagatg cggtggcaaa cgtcttaacc
     1381 atgacgttgc tgaaaaaagc gcagttgaag tccacggtga tgccacaggt tcagcaatat
     1441 gcgtgggata aagcggcaaa cagcaatcag ccgctggcgc acacggttgt gctgctcaat
     1501 agcggtggtg acgctaccca gacggccgct attttaatgg tttgaccgct gagcaatcca
     1561 ctattgagcg cgcgctggcc atgaactggc tggcgaaata tatggcgaca atgcctccag
     1621 ttgttttgcc tgcgcctgcg ggcgcatggg ctaaacataa gttaactgga gggggcgaag
     1681 actggcgttg ggttggtcag ggtgtgccgg acattctctc ttttggtgac gaattatcgc
     1741 cgcaaaatgt gcaggtccgc tggcgtgaac cggcaaaaac ggctcaacaa agtaacattc
     1801 cggtgaccgt tgaacgccag ttgtatcggc ttatccccgg tgaagaagag atgagcttta
     1861 ctctgcagcc ggtgaccagc aatgagattg acagcgatgc gctgtatctc gatgaaatta
     1921 cgcttaccag cgagcaggat gcagttctgc gctatggtca ggtggaagta ccgctcccgc
     1981 cgggagccga cgttgagcgc acaacatggg gcatttcagt caataaaccc aacgccgcga
     2041 aacagcaggg gcaattgctg gaaaaagcgc gtaatgaaat gggcgaactg gcctatatgg
     2101 tgccggtgaa agaactgacg ggaacggtca ctttccgcca tttgctgcgc ttctcgcaaa
     2161 aagggcaatt cgttctgcct cctgctcgtt atgtgcgttc ctatgcacct gcgcagcaaa
     2221 gtgtcgcggc agggagtgaa tggaccggga tgcaggtgaa ataagtgaac tggcgcagaa
     2281 tcgtctggct attggcgttg gttactctgc caacgcttgc ggaggagacg ccgttacaac
     2341 tggtgctgcg cggtgcgcag cacgatcaac tgtatcagct gtcgtcatca ggcgtgacca
     2401 aagtctcagc attacctgac tcgctcacca cgccgttagg gagtttgtgg aaactgtatg
     2461 tttacgcctg gctggaggat acccatcaac cggagcagcc ctaccagtgt cgcggaaact
     2521 ccccggaaga ggtttattgt tgtcaggctg gggagagcat aacgcgcgat accgcgctgg
     2581 tacgttcctg tgggctctat tttgcgccgc agcggttgca tattggcgca gatgtgtggg
     2641 ggcaatactg gcaacagcgt caggcacctg cgtggttagc gtctcttaca acgctgaagc
     2701 cagaaacctc ggtaacggtt aaatcgctgc ttgattcatt agcgacgtta cctgcgcaaa
     2761 acaaagctca ggaagtctta ctggatgtgg tgctggacga ggcgaaaatc ggtgtcgcat
     2821 ccatgctcgg tagtcgggta cgggtgaaaa cctggagctg gtttgccgat gataaacagg
     2881 aaatccgtca gggtggtttt gctggctggt taaccgatgg tacaccgctg tgggttactg
     2941 gtagtggcac cagcaaaacg gtactaaccc gttatgcgac ggtgcttaat cgggttttgc
     3001 cagtgccgac gcaggttgcc agtgggcaat gtgtagaggt ggagctgttt gcccgctatc
     3061 cgctgaagaa aatcacagcg gaaaagagta cgacggcagt aaaacccagc gtacttaatg
     3121 ggcgctatcg tgtgacgttt actaatggta accacattac gtttgtcagc cacggtgaga
     3181 cgaccttact gagcgaaaag ggcaaactga aattgcagtc gcatctcgat cgtgaagagt
     3241 acgttgccag agttctggat cgcgaggcga aaagtacgcc acctgaggca gcgaaagcca
     3301 tgaccgtggc gatacgtact tttttacagc aaaacgccaa tcgggaaggt gattgcctga
     3361 ccatccccga cagcagcgcc acgcagcgtg tttctgcttc gccagcgacc acgggggcgc
     3421 gaacgatgac cgcctggacg caggagctca tctatgcagg cgatccggtt cattatcatg
     3481 gcagtcgcgc taccgaaggg acactttcct agcggcaagc cacggcccag gcggggcagg
     3541 gggagcgtta cgaccagata ctcgcgtttg cttatcccga caacagcctt agccgctggg
     3601 gagctccacg ctcaacctgc cagttattac ccaaagcgaa agcctggctg gcgaaaaaaa
     3661 tgccgcagtg gcggcgtata ttacaaggcg agacggggta caacgaacca gacgtgtttg
     3721 cggtctgtcg tttagtctca ggtttcccct ataccgatcg gcagcagaaa cggctgttta
     3781 ttcgtaactt ctttacgctt caggatcggc tggatttaac ccatgagtat ctgcacctgg
     3841 cctttgatgg ttatcccacc gggcttgatg agaactatat cgaaacgctg acccgacaat
     3901 tattaatgga ctaatgctat gcgaaagatt tttcttccgt tgttactggt ggcactttcg
     3961 cctgtcgctc acagtgaagg tgtgcaggaa gtcgagattg acgcaccgct ctctggctgg
     4021 catccggcag agggcgaaga tgccagtttt tcacaatcga tcaattaccc ggcgtcgtcc
     4081 gtcaacatgg cagatgatca aaatatctca gcgcagatcc gcggcaaaat aaagaattat
     4141 gctgcggcgg gtaaagttca gcagggccgg ctggtggtca acggtgccag tatgccgcag
     4201 agaattgaat ccgatggttc atttgcacgc ccttatattt tcactgaagg cagcaacagc
     4261 gtgcaggtca tcagcccgga tgggcaaagc cggcaaaaaa tgcagtttta ctcaacgccg
     4321 ggtacaggaa ctattcgtgc acgtttacgg ctggttctct cgtgggatac ggacaatacc
     4381 gatctcgatc ttcatgttgt cacgcccgat ggcgaacacg cctggtacgg taacaccgtg
     4441 ctgaaaaaca gtggtgcact ggatatggat gtcacgacgg ggtacggacc cgagattttc
     4501 gccatgccag caccagttca cgggcgctat caggtgtata tcaactacta tggtgggcgc
     4561 agcgaaacgg aattgacgac cgcccaactg acgctgatca ccgatgaagg atcggtcaat
     4621 gagaaacagg aaacatttat tgtaccgatg cgtaatgctg gcgagctgac gctggtgaaa
     4681 agttttgact ggtgatccat cccttcacac aacctggctc tctctttttt cagataagac
     4741 tgctgggcga tctgctgacg ccaggatata accattaaca agcgtcggca gattggtcat
     4801 ttcttatttt tcgtactgag aactgtccat ctactccggc tttcctggtt aaggcaatgg
     4861 tttctaagcg atacgcatta ttgcaatggt gacagaacat tgcggtaaca cgcttttacc
     4921 gctaccttaa ccacactcca tcggtcacct gaggcggagc ttcgcccctt tgaaatacct
     4981 tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg ttcagagcat tggcgttagt
     5041 gctctggctg ttgattgctt tttcatccgt tttttacatc gttaatgcgt tacatcagcg
     5101 agaatcggaa attcgtcagg aatttaatct gagttccgat caggctcagc gctttattca
     5161 acgcacctct gatgtgatga aagagcttaa gtacatcgcc gaaaatcgct tatcggcaga
     5221 aaacggtgtg ctttccccgc gtggacgaga aacgcaggcg gatgtgcctg cgtttgaacc
     5281 gctgtttgcc gactccgatt gttccgcaat gagtaacacc tggcgaggtt ctctggagtc
     5341 attggcgtgg tttatgcgct actggcgcga taatttttct gcggcttacg atctcaaccg
     5401 ggtattttta atcggcagtg ataacctctg catggccaat ttcggtctgc gtgatatgcc
     5461 agtggaacgc gataccgcgt tgaaagcgtt gcatgaacgc atcaataaat atcgaaatgc
     5521 accacaagat gatagcggca gtaacctcta ctggatcagc gaaggtccgc gccctggcgt
     5581 cgggtatttt tacgcgttga cgccagttta tctggcgaac cggttgcagg cgcttttggg
     5641 tgtcgagcag accatccgga tggagaactt tttcttaccg ggtacgttgc cgatgggggt
     5701 taccattctt gatgaaaatg gtcataccct gatttcgctt accggaccag aaagtaaaat
     5761 taagggcgat cctcgctgga tgcaggaacg ctcttggttt ggctatacgg aagggttccg
     5821 ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta agcatcgtgt attcggtgcc
     5881 ggttgataag gtgctggaac gcattcgcat gttgatcctt aacgcaattt tgctgaatgt
     5941 gcttgccgga gctgcattgt ttactctcgc gcggatgtac gagcgacgta ttttcattcc
     6001 ggcggaaagc gacgccctgc gactggaaga acatgagcag ttcaatcgca agattgtcgc
     6061 ctccgcgcca gtgggtatct gcattttgcg taccgctgat ggcgtcaata ttttaagtaa
     6121 cgaactggcg catacctatc tcaatatgct tacgcatgag gaccgccaac gactgacgca
     6181 aattatctgt gggcagcagg tcaattttgt tgatgtcctg accagcaaca ataccaatct
     6241 gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac gtggccattt gtgtgctggt
     6301 ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag gagatggcac aagcagcgga
     6361 acaggcgagc cagtcaaaat cgatgttcct tgccaccgtc agtcatgagc tgcgaacgcc
     6421 gctgtatggc attatcggta acctggatct gttgcaaacc aaagagttac cgaaaggcgt
     6481 cgatcgtctg gtgacggcaa tgaacaactc ttccagcctg ttgttgaaaa ttatcagcga
     6541 tattctcgat ttctcgaaga ttgaatcgga acagttgaag atcgaaccgc gtgagttttc
     6601 accgcgtgaa gtgatgaacc acatcaccgc caactattta ccgctggtgg tacgcaagca
     6661 gttaggcttg tactgcttta ttgaaccgga tgtgccagtg gccttaaatg gcgacccgat
     6721 gcgtttacag caggtcatct ccaacctgtt gagtaatgcc ataaaattca ccgataccgg
     6781 ctgtatagtt ttgcatgttc gcgctgatgg cgattatctc tctatccgtg ttcgcgatac
     6841 cggcgtgggg attccggcga aagaagtggt gcgcttgttt gatcccttct tccaggtcgg
     6901 aacgggcgta cagcgaaact tccaggggac cggtctgggt ctggcgattt gtgaaaaact
     6961 gatcagcatg atggacggcg atatctcggt agattcagaa ccgggaatgg gcagccagtt
     7021 taccgtgcgt attccgttgt acggcgctca gtacccgcag aaaaaaggcg tggaagggtt
     7081 gagtggtaaa cgctgctggc tggcggtccg caatgcgtcg ctctgtcagt tcctggaaac
     7141 cagtttgcag cgcagcggca tcgtcgttac aacatacgaa gtgcaggaac cgactcccga
     7201 agatgtgtta atcactgacg aggtagtgag taaaaaatgg cagggcagag cggtagtgac
     7261 cttctgtcgt cgccatattg gtattccgct ggagaaagcg ccaggggagt gggtacacag
     7321 tgtggctgct ccgcatgagc taccggcatt gttggcgcgt atttatttga tcgagatgga
     7381 gagcgacgat cctgctaacg ctctgccgtc gacggacaaa gcggtcagcg ataacgacga
     7441 tatgatgatt ctggtcgtgg atgatcatcc gattaaccga cgtctgctgg cagatcagtt
     7501 gggatcgttg ggctatcaat gtaaaaccgc gaatgatggc gtcgatgcgc ttaatgtact
     7561 tagtaagaat catattgata tcgtgcttag cgacgtcaac atgccaaata tggatggtta
     7621 ccgcttgacg caacgcattc gtcagttggg actgacgttg ccggtaatcg gagtaactgc
     7681 taatgcgttg gctgaagaga agcagcggtg tctggagtcc ggtatggaca gctgcctctc
     7741 gaagccggta acgctggatg tgataaaaca gacgctgacg gtatatgccg agagggtcag
     7801 gaaatcgcgg gattcgtagg acggataagg cgtttacgcc gcatccggca ttcagtgcaa
     7861 atgccagata agacactgac gcgtcttatc tggcctactt taatgtaatc tgttgaaata
     7921 atgggaatcg taggccggat aaggcgttta cgccgcatcc ggcaatcggt gcaaatgcca
     7981 gataagacac taacgcgtct tatctggcct acagatgatt agtctttatc tgccggactt
     8041 aaggtcacag aagagagata attcagcagg gcgatatcgt tctcgacgcc cagcttcatc
     8101 atcgcagatt tcttctggct actgatggtt ttaatactgc ggttcagctt tttagcgatc
     8161 tcggtcacta ggaagccttc cgcaaacagg cgcagaactt cactctcttt tggcgagaga
     8221 cgcttgtcac cgtaaccacc agcactgatt ttttccaaca ggcgagaaac gctttccggg
     8281 gtaaatttct tccctttctg cagcgcggcg agagctttcg gcagatcggt cggtgcacct
     8341 tgtttcagca cgatcccttc gatatccaga tccaataccg cactaagaat cgccgggttg
     8401 ttgttcatag tcagaacaat gatcgacagg cttgggaaat ggcgcttgat gtacttgatt
     8461 aaggtaatgc catcgccgta cttatcgcca ggcatggaga gatcggtaat caacacatgc
     8521 gcatccagtt tcggcaggtt gttgatcagt gctgtagagt cttcaaattc gccgacaaca
     8581 ttcacccact caatttgctc aagtgatttg cgaataccga acaagactat cggatggtca
     8641 tcggcaataa ttacgttcat attgttcatg taataggcta ccttgctaca gcaagctctt
     8701 gacataactg tcaatgtcgc tgatgtattt ttctattcct ggaacatcct tctcacgaat
     8761 cagatgttcc agcgtttcac ataactgctt gccgggtacc agatttagca tggcaaatac
     8821 gcctttaaga cgatgagccg tttgggctaa cgcagcaaag tcactggttg ctgcttcagt
     8881 atacagcctc ttaacatcat ccggtactgt gtctacaaag agcgcataat agccgctggc
     8941 atggagttgc gcattttcat ctccgcccag aggcgattct gtcacctctt cctgcgccag
     9001 ttgcacttca attaattgta agaccgcttc ctgcatagcg ttgctcatat tgaagttgac
     9061 gcacaattga ccaggcccaa tttcccgtac gtcagactca tcatcgctta aaagcaagcc
     9121 agaggcagta agattagacg gattatccgt taaaaagata tcataatctt gactaattaa
     9181 tctttcatcg ggtgtgatac aggttgcacc ccaattttct aacaggcgag tgacaatatt
     9241 ccgaatttct gccgaagtaa catccaccat tacgcagaca tcatccagta aacgctcttc
     9301 ttcctcttca acttccgggt cagcggcgag cattttgata tgcacagagt agcgtgtacc
     9361 aagcccatcc cgcgttttga tgtttaaatg accgcccagt ttacgtgcca gttgatcgct
     9421 cagccagaat gccagcgggt ccgcctttcc ataacgatcg ttttgggtct ggttgataaa
     9481 cgggaagtgc aaattatcca tttcatgaat acttacgcct tctcccgtgt ccagaatgcg
     9541 gaatgtcagg cggtcttcag aggactcatc ctgatcaacc tcaagggtga tttttcccaa
     9601 ttgcgttgag gtcacggcat attgcatcag tagcagcaaa atacgtcgta aggcatcgcg
     9661 atcgccgcgg cgcatatcgt gtgctttcag atgattgtta atcagcagtt gcagaccttt
     9721 acgcttgatg gcaggcaaca ctgaaggcac aacttcatca attaaatcct gcacggagaa
     9781 cagcaccgtc tcacttttcc agctatcatc cgcaagcatg ttcgctaact gtatttcttc
     9841 gaccagccgc accagcacat ctgcctgatt cgccagttgt ttgctttccg gggcgttgag
     9901 tttagccgca ctctccgcca gggactgtgc gggttctttc agcgcatcgc caatgttttt
     9961 cataaaggtc atccgcccct gctggttttt ctcatacaga cgctgcgcct gcttgagttt
    10021 cttgtttacc agcacttcgc gatcctgatc gcgaataatg aaaatttgtg tgcgcggcgc
    10081 gacctggctg cggaacatgc ggatctcata cagctcgtta ttgatcgtcg cctgaataat
    10141 cccctgatgc tgttccgcca tggtggtgat gttttgcaga ttcagatgcg gcagcaaatg
    10201 atcggcaatt ttgttactta tgacagtgcg gttcgattcc tgatcgtgaa ccagcaggcc
    10261 gagcggcaac agtgagacta tctcttcatt gattgcccgt aaaatgcgca attcgttatt
    10321 gaccgccgtg ctggggacgc tttctgtact gcggctggag aaatggcgga atgtggtata
    10381 gccaaataac gccagcgcca gcaaaccgat gttcagcagc agtggcagca gaatgttttg
    10441 caacgtatcc agcaataagg tgccataagg aacctgccag accaggcgca tatcggtaga
    10501 gttgagtgcc gaggagattt caatcttcgt actgttaaag tggatactga cgctatccgt
    10561 cccttctttc tcattatcat tgtttcccgt tgccgtcgcg tctggctcaa ggcggaaact
    10621 gtccagcggc atacccggtg ggatcaaatc attaatcggc agatcaaaag ccacgaccgt
    10681 tgccagatgt cccggctggt tgaacgtggt acgcaaggta aagtaatgac cgttctgcca
    10741 ggccaggcgg cgcatgttag aaaaactttc gcgttcatcg agggcgttgg cctgttgcaa
    10801 catttctgca cgacgtgaat caacaatgtc actgacggtc gattctttaa atccggaggt
    10861 gagatcttta aggggtaggg ttgagatcag caccagactg ttatcctgac cattcaggta
    10921 atacatcgac cacggtacat tttctgcacc ccacaatgta tccagataag tggacatccg
    10981 cagagtcatc tcaagcgttg agttgtcgtg agagccaaag attaacgctt ccgttttgcg
    11041 gcgtggtttc tccagatagt agacatcctg tttcaggcgc gtctcttgta aaccttcgcc
    11101 ggaggagggg gaggtcgtcg cggcaatgtt gtcgtagatc tgccaggtca cgtaacgcca
    11161 ggtatcgacg cgcttttgga ttgcgtgggt aatgtcgaca atctggtaac ttttatcttt
    11221 cagccaggcg ttaacggcac tttgtaccat tacccccatc gtcaccagta acacaatgat
    11281 caacagtaaa aagaagcggg taatgctccc cggtaggagt gaaaagcggg tcgtggccgt
    11341 tgtctctttc tgacgcattc gtgtttatga cctgttaaaa cttcgcgaag tgatagggca
    11401 gtataaaggg tacagtgtga aattccagac tcttacctga ttgatgattc tctattctcc
    11461 cgtgatattt atcatcctgt atggggggaa atagatgtac aagcgccatt ttgctgtaca
    11521 aataacctac aaaaagccca acgaaattaa ttgcaaataa acaaagattg ctggaaatta
    11581 tgctgatgtt aattatttgt gaaatagtta acaagcgtta tagtttttct gtggtagcac
    11641 agaataatga aaagtgtgta aagaagggta aaaaaaaacg aatgcgaggc atccggttga
    11701 aataggggta aacagacatt caga
//
LOCUS       DADLSA010000119        11664 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.119, whole genome shotgun sequence.
ACCESSION   DADLSA010000119 DADLSA010000000
VERSION     DADLSA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.119"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..1614
                     /gene="ftsK"
                     /locus_tag="JN691_003119"
     CDS             <1..1614
                     /gene="ftsK"
                     /locus_tag="JN691_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY7244494.1"
                     /translation="QPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKP
                     TTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFE
                     LNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVL
                     DNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLY
                     KAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALLWCVNEMERRYKLMS
                     ALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADL
                     MMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKI
                     DSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQY
                     VDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAAR
                     IIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            1769..2380
                     /gene="lolA"
                     /locus_tag="JN691_003120"
     CDS             1769..2380
                     /gene="lolA"
                     /locus_tag="JN691_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAY7244495.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            2391..3734
                     /locus_tag="JN691_003121"
     CDS             2391..3734
                     /locus_tag="JN691_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAY7244496.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            3825..5117
                     /gene="serS"
                     /locus_tag="JN691_003122"
     CDS             3825..5117
                     /gene="serS"
                     /locus_tag="JN691_003122"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAY7244497.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            5357..7801
                     /gene="dmsA"
                     /locus_tag="JN691_003123"
     CDS             5357..7801
                     /gene="dmsA"
                     /locus_tag="JN691_003123"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HAY7244498.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNHTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            7812..8429
                     /gene="dmsB"
                     /locus_tag="JN691_003124"
     CDS             7812..8429
                     /gene="dmsB"
                     /locus_tag="JN691_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY7244499.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            8431..9294
                     /locus_tag="JN691_003125"
     CDS             8431..9294
                     /locus_tag="JN691_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase"
                     /protein_id="HAY7244500.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(9330..9956)
                     /locus_tag="JN691_003126"
     CDS             complement(9330..9956)
                     /locus_tag="JN691_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7244501.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            10271..11419
                     /locus_tag="JN691_003127"
     CDS             10271..11419
                     /locus_tag="JN691_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7244502.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNSSIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         2772 a         2822 c         3158 g         2912 t
ORIGIN      
        1 cagccgcaac aaccggttgc accgcagccg cagtatcagc agccacaaca gccagttgcg
       61 ccacagccgc aggataccct gcttcatccg ctgttgatgc gtaatggcga cagccgtccg
      121 ttgcacaaac cgacgacgcc gctgccttct ttggatttgc ttacaccgcc gccgagcgaa
      181 gtggagccgg tagatacctt tgcgcttgaa caaatggcgc gtctggtgga agcgcgtctg
      241 gctgatttcc gtattaaagc cgatgtcgtt aattactctc cggggccagt tatcactcgc
      301 tttgaactta acctggctcc tggcgtaaaa gcggcgcgca tttctaactt gtcacgggac
      361 cttgcccgtt cactttcgac ggtggcggtg cgtgtcgttg aagttattcc tggcaaaccc
      421 tatgtaggtc tggagttacc aaataaaaaa cgacaaaccg tttatctgcg cgaagttttg
      481 gataacgcca aattccgcga taatccgtcg ccattaaccg tggtgctggg taaagatatc
      541 gccggtgagc cggtggttgc cgatctggcg aaaatgccgc acttgttggt tgcggggact
      601 accggttccg gtaaatctgt cggtgtgaac gcgatgatcc tgagcatgct ttataaagca
      661 cagccagaag atgtgcgttt catcatgatc gacccgaaaa tgctggagct ttcggtttat
      721 gaaggcattc cgcacctgtt aacggaagtc gttactgata tgaaagatgc cgccaacgcg
      781 ctgctctggt gtgttaacga gatggagcgg cggtataaac tgatgtctgc gctgggtgtg
      841 cgtaatctgg cgggttataa cgaaaaaatt gctgaagccg atcgcatgat gcgtccgatt
      901 ccagacccgt actggaagcc gggtgacagt atggatgccc agcatccggt gctgaaaaaa
      961 gaaccataca ttgtcgtgtt ggttgacgaa tttgccgacc tgatgatgac ggtaggtaaa
     1021 aaagtggaag agctgatagc acgtctggcg caaaaagccc gtgccgcggg tatccacctc
     1081 gtactggcaa ctcagcgtcc atcggttgat gttattactg gtctgattaa agcgaatatt
     1141 ccgacccgta tcgcctttac cgtatccagt aagattgact cacgtaccat tcttgatcag
     1201 gctggcgcgg aatcactgct gggtatgggg gatatgctct actctgggcc gaactccacg
     1261 ttgccggtac gtgtccatgg tgcttttgtt cgcgatcagg aagttcatgc cgtggtgcag
     1321 gactggaaag cgcgtggtcg cccacagtat gttgatggca tcacctccga cagcgaaagc
     1381 gaaggtggtg cgggtggttt cgatggcgct gaagaactgg atccgttgtt cgatcaggcg
     1441 gtgcagtttg tcactgaaaa acgcaaagcg tcaatttctg gcgtacagcg tcagttccgc
     1501 attggttata accgtgcagc gcgtattatc gaacagatgg aagcgcaggg gattgtcagc
     1561 gaacaggggc acaacggtaa tcgtgaagtg ctggccccac cgccgtttga ctaattaatg
     1621 cattgccgga taaggcgcgg tagcgtcgca tccggcactc tatcaactga aaattcagta
     1681 ttttcttctt tcctcaagct gattattagc ctggaataga gagtagaggg aactcccgat
     1741 cgggagtgac gtaatttgag gaataatgat gaaaaaaatt gccatcacct gtgcattact
     1801 ctcaagctta gtagcaagca gcgtttgggc tgatgccgca agcgatctga aaagccgcct
     1861 ggataaagtc agcagcttcc acgccagctt cacacaaaaa gtgactgacg gtagcggcgc
     1921 ggcggtgcag gaaggtcagg gcgatctgtg ggtgaaacgt ccaaacttat tcaactggca
     1981 tatgacacaa cctgatgaaa gcattctggt ttctgacggt aaaacactgt ggttctataa
     2041 cccgttcgtt gagcaagcta cggcaacctg gctgaaagat gccaccggta atacgccgtt
     2101 tatgctgatt gcccgcaacc agtccagcga ctggcagcag tacaatatca aacagaatgg
     2161 cgatgacttt gtcctgacgc cgaaagccag caatggcaat ctgaagcagt tcaccattaa
     2221 cgtgggacgt gatggcacaa tccatcagtt tagcgcggtg gagcaggacg atcagcgcag
     2281 cagttatcag ctgaaatccc agcaaaatgg ggctgtggat gcagcgaaat ttaccttcac
     2341 cccgccgcaa ggcgtcacgg tagatgatca acgtaagtag aggcacctga gtgagcaatc
     2401 tgtcgctcga tttttcggat aatacttttc aacctctggc cgcgcgtatg cggccagaaa
     2461 atttagcaca gtatatcggt cagcaacatt tgctggctgc ggggaagccg ttgccgcgcg
     2521 ctatcgaagc cgggcatttg cattctatga tcctctgggg gccaccgggt accggcaaaa
     2581 caaccctcgc tgaagtgatt gcccgctatg cgaacgctga tgtggaacgt atttctgccg
     2641 tcacctctgg cgtgaaagag attcgcgagg cgatcgagcg cgcccggcaa aaccgcaatg
     2701 caggtcgccg cactattctt tttgttgacg aagttcaccg tttcaacaaa agccagcagg
     2761 atgcatttct gccacatatt gaagacggca ccatcacttt tattggcgca accactgaaa
     2821 acccgtcgtt tgagcttaat tcggcactgc tttcccgtgc ccgtgtttat ctgttgaaat
     2881 ccctgagtac agaggatatt gagcaagtac taactcaggc gatggaagac aaaacccgtg
     2941 gctatggtgg tcaggatatt gttctgccag atgatacacg acgtgccatt gcagaactgg
     3001 tgaatggcga cgcgcgccgg gcgttaaata cgctggaaat gatggcggat atggccgaag
     3061 tctatgatag cggtaagcgg gtcctgaagc ctgaattact gaccgaaatc gccggtgaac
     3121 gtagcgcccg ctttgataac aaaggcgatc gcttttacga tctaatttcc gcactgcata
     3181 agtcggtacg tggtagcgca cccgatgcgg cgctgtactg gtatgcgcga attattaccg
     3241 ctggtggcga tccgttatat gtcgcgcgtc gctgtctggc gattgcgtct gaagacgtcg
     3301 gtaatgccga tccacgggcg atgcaggtgg caattgcggc ctgggattgc tttactcgcg
     3361 ttggcccggc ggaaggtgaa cgcgccattg ctcaggcgat tgtttacctg gcctgcgcgc
     3421 caaaaagcaa cgctgtctac actgcgttta aagccgcgct ggccgatgct cgcgaacgcc
     3481 cggattatga cgtgccggtt catttgcgta atgcgccgac gaaattaatg aaggaaatgg
     3541 gctacgggca ggaatatcgt tacgctcatg atgaagcaaa cgcttatgct gccggtgagg
     3601 tttacttccc gccggaaata gcacaaacac gctattattt cccgacaaac aggggccttg
     3661 aaggcaagat tggcgaaaag ctcgcctggc tggctgaaca ggatcaaaat agccccataa
     3721 aacgctaccg ttaatgttat cgttgcggta atgttgttac tgtatccctg tggtcgcagg
     3781 ctgtggccac atctcccatt taattcgata agcacaggat aagcatgctc gatcccaatc
     3841 tgctgcgtaa tgagccagac gcagtcgctg aaaaactggc acgccggggc tttaagctgg
     3901 atgtagataa gctgggcgct cttgaagagc gtcgtaaagt attgcaggtc aaaacggaaa
     3961 acctgcaagc ggagcgtaac tcccgatcga aatccattgg ccaggcgaaa gcgcgcgggg
     4021 aagatatcga gcctttacgt ctggaagtga acaaactggg cgaagagctg gatgcagcaa
     4081 aagccgagct ggatgcttta caggctgaaa ttcgcgatat cgcgctgacc atccctaacc
     4141 tgcctgcaga tgaagtgccg gtaggtaaag acgaaaatga caacgttgaa gtcagccgct
     4201 ggggtacccc gcgtgagttt gactttgaag ttcgtgacca cgtgacgctg ggtgaaatgc
     4261 actctggcct cgactttgca gctgcagtta agctgactgg ttcccgcttt gtggtaatga
     4321 aagggcagat tgctcgcatg caccgcgcac tgtcgcagtt tatgctggat ctgcataccg
     4381 aacagcatgg ctacagtgag aactatgttc cgtacctggt taaccaggac acgctgtacg
     4441 gtacgggtca gctgccgaaa tttgctggcg atctgttcca tactcgtccg ctggaagaag
     4501 aagcagacac cagtaactat gcgctgatcc caacggcaga agttccgctg actaacctgg
     4561 tgcgcggtga aatcatcgat gaagatgatc tgccaattaa gatgaccgcc cacaccccat
     4621 gcttccgttc tgaagccggt tcatatggtc gtgacacccg tggtctgatc cgtatgcacc
     4681 agttcgacaa agttgaaatg gtgcagattg tgcgcccaga agactcaatg gcggcgctgg
     4741 aagagatgac tggtcatgca gaaaaagtcc tgcagttgct gggcctgccg taccgtaaaa
     4801 tcatcctttg cactggcgac atgggctttg gcgcttgcaa aacttacgac ttggaagtat
     4861 ggatcccggc acagaacacc taccgtgaga tctcttcctg ctccaacgtt tgggatttcc
     4921 aggcacgtcg tatgcaggca cgttgccgca gcaagtcgga caagaaaacc cgtctggttc
     4981 ataccctgaa cggttctggt ctggctgttg gtcgtacgct ggttgcagta atggaaaact
     5041 atcagcaggc tgatggtcgt attgaagtac cagaagttct gcgtccgtat atgaacggac
     5101 tggaatatat tggctaatac ccaatttttc tgaatctaaa aagcgccttt agggcgcttt
     5161 ttttgtctcc ctttgatacc gaacaataat tactcctcac ttacacgtaa tactactttc
     5221 gagtgaaaat ctacctatct ctttgatttt caaatcattc gatgtataca agcctatata
     5281 gcgagctgct atagaaataa ttacacaata cggtttgtta ctggaatcaa tcgtgatcaa
     5341 gcttgagtga gccattatga aaacgaaaat ccctgatgcg gtattggctg ctgaggtgag
     5401 tcgccgtggt ttggtaaaaa cgacagcgat cggcggcctg gcaatggcca gcagcgcatt
     5461 aacattacct tttagtcgga ttgcgcacgc tgtcgatagc gccattccaa caaaatcaga
     5521 cgaaaaggtt atctggagcg cctgtacagt taactgtggt agtcgctgcc cgctacgtat
     5581 gcacgtcgtg gacggtgaaa tcaaatatgt cgaaacggac aataccggcg atgacaatta
     5641 cgacggcctg caccaggttc gcgcctgcct gcgtgggcgt tccatgcgtc gccgtgtcta
     5701 caatccggac cgcctgaaat atccgatgaa acgagtcggg gcgcgcggtg aaggcaaatt
     5761 cgagcgcatt agctgggaag aagcctacga catcatcgcg accaatatgc agcgcctgat
     5821 caaagagtac ggcaacgagt ctatctatct gaactatggc accggtacgc tgggcggcac
     5881 catgacccgc tcctggccgc cgggaaatac cctggtcgcg cgcctgatga actgctgcgg
     5941 cggttatctt aaccattacg gtgactactc ttccgcgcaa attgcggaag gtttgaacca
     6001 tacctacggc ggctgggcag atggtaacag cccgtcggat atcgaaaaca gtaaattggt
     6061 agtgctgttt ggtaataacc caggcgaaac gcgaatgagt ggcggtgggg tgacttacta
     6121 tcttgaacag gcacgccaga aatctaatgc tcgcatgatc atcatcgatc cgcgctatac
     6181 cgacaccggt gccgggcgcg aagatgagtg gatccctatt cgtccaggaa cagatgccgc
     6241 actggttaac ggtctggcgt acgtcatgat cactgaaaac ctggtggatc aggcattcct
     6301 cgataaatat tgcgttggct atgacgagaa aaccctgccc gccagtgcgc cgaaaaatgg
     6361 tcattataaa gcttatattc tgggtgaagg gccagatggc gtggctaaaa caccggaatg
     6421 ggcctcgcaa atcaccggtg ttccggcaga caaaatcatc aaactggctc gtgaaatcgg
     6481 cagtaccaaa ccggcgttta tcagccaggg atggggcccg cagcgtcacg ctaacggtga
     6541 aatcgcaacc cgtgctatct cgatgctggc gattctgacc ggtaacgttg gtattaatgg
     6601 agggaacagc ggcgcgcgtg aaggttcata cagcttaccg tttgtccgta tgccgacctt
     6661 ggaaaacccg atccagacca gcatttcgat gtttatgtgg accgatgcca ttgaacgtgg
     6721 cccggaaatg acggcgctgc gtgatggtgt gcgcgggaaa gataagctgg atgtgccgat
     6781 caaaatgatc tggaactatg ccggtaactg cctgattaac cagcattctg aaatcaaccg
     6841 tacccatgaa atcctccagg atgataagaa gtgcgagctg attgtggtta tcgactgcca
     6901 catgacctca tcggcgaaat atgctgacat cctgctgcct gactgcaccg cttccgaaca
     6961 gatggacttt gcactggatg catcctgcgg gaatatgtct tacgtgattt tcaacgatca
     7021 ggtgattaaa ccgcgctttg aatgtaagac catctatgaa atgaccagcg aactggcaaa
     7081 acgtcttggc gttgagcaac agtttactga aggccgtacc caggaagagt ggatgcggca
     7141 tctgtatgcc cagtcgcggg aagcgattcc tgaactgcca acgtttgaag agttccgcaa
     7201 gcaggggatc tttaaaaagc gcgacccaca agggcatcac gttgcttata aagccttccg
     7261 tgaagatccg caggcaaacc cactgactac gccatcgggc aaaattgaga tttattcgca
     7321 ggcgctggct gacattgccg ctacctggga attgccagaa ggcgatgtga tcgatccact
     7381 gccgatctac acgccgggct ttgaaagtta tcaggatccg ctgaacaaac agtatccgct
     7441 gcagcttaca ggtttccact ataaatctcg cgttcactca acttacggca acgttgatgt
     7501 gctgaaagcg gcttgccgtc aggaaatgtg gatcaacccg cttgatgccc aaaaacgcgg
     7561 tatccacaac ggcgataaag tcaggatctt taacgatcgt ggtgaggttc atattgaggc
     7621 gaaagtgacg ccacgaatga tgccgggcgt ggtcgcactg ggtgaaggcg cctggtatga
     7681 cccggatgca aaacgtgtcg ataagggcgg ttgtatcaac gtactgacca ctcaacgtcc
     7741 gtctcctctc gctaagggga atccgtcaca tacaaacctt gttcaggttg aaaaggtgta
     7801 aggagtaacc gatgacaacc cagtatggat tttttattga ttccagccgt tgcaccggtt
     7861 gcaaaacctg cgagctggct tgtaaagact acaaagattt gacgccagaa gtcagtttcc
     7921 gccgcattta tgaatatgct ggcggcgact ggcaggaaga taacggtgtc tggcaccaga
     7981 acgtgtttgc ctactatctg tcgatttcgt gcaaccactg cgaagatccg gcttgtacta
     8041 aagtctgccc gagcggtgcg atgcataaac gcgaagatgg ttttgttgtg gtcgatgaag
     8101 atgtatgcat tggctgccgt tactgtcata tggcttgccc gtatggcgca ccgcaatata
     8161 acgaaacgaa aggccatatg accaaatgcg atggttgtta tgaccgtgtt gcggagggta
     8221 aaaagccgat ctgtgttgaa tcttgcccgc tgcgggcgct ggatttcggc cctatcgacg
     8281 agctgcgtaa aaaacatggc gatctggcgg ccgttgcgcc gttgccgcga gctcacttta
     8341 ccaaaccgaa tattgtgatc aaacccaatg ccaatagccg cccgaccggg gataccactg
     8401 gctatctggc aaacccgaag gaggtgtgag atgggaagtg gatggcatga atggccgctg
     8461 atgatcttca cggtcttcgg gcaatgtgtg gcgggtggtt ttatcgttct ggctttggcg
     8521 ctgctcaaag gcgacctgcg agcagaagcc cagcagcgtg ttatcgcctg catgtttggc
     8581 ttatgggtac tgatgggcat tggctttatc gcctctatgc tccatcttgg ttcaccgatg
     8641 cgcgccttta actcgctcaa ccgggtaggg gcttcagcac tcagtaacga aatcgccagc
     8701 ggttcgatct tttttgctgt aggcggcatc ggctggttgc tggcaatgct gaaaaagctg
     8761 tcaccggcat tgcgtacgct atggctggta gtgacgatgg ttcttggcgt cgtctttgtc
     8821 tggatgatgg tgcgtgtgta taacagcatt gataccgttc cgacctggta cagcatctgg
     8881 acgccgatgg gcttcttcct gacgatgttc atgggcggcc cgttactggg ttacctgttg
     8941 ctgagtctgg ctggcgtcga tggctgggcg atgcgtctgc tgccagccat ttctgtactg
     9001 gcactggtgg tgagtggcgt gatgtcagtg atgcagggca cagagctggc aactattcat
     9061 agttctgtgc agcaggccgc agcgctggtg ccggactatg gtgcgctgat gtcctggcgg
     9121 atcgtgcttt tggccgttgc cctgtgcttg tggattgcac cacagctaaa aggttatcag
     9181 cctgcggttc cgctactatc cgtctcattc attctgcttc tggcagggga attaatcggt
     9241 cgcggcgtat tctatggttt gcatatgacc gtggggatgg ccgtcgcaag ctaatcagta
     9301 acaaccgggg tttcggcccc ggttctcttt tatttctgct tcgttaacgt gtcataactg
     9361 gtcatcaaat tacgataatc aggaatatgg ttagagaaca acgtcgccag tccttcaata
     9421 tcattacgcc agtcacggtg cagctcgcaa gccacgccga accatgtcat caattgcgca
     9481 ccagcttgcg acatgcgatc ccacgccgaa tgccgggtaa tttcattaaa ggtccctgat
     9541 gcatcagtca cgacaaagac atcaaaccct tcttcaatgg ctgaaagcgc cgggaatgcc
     9601 acgcaaactt cggttaccac accggcaata attaactgtt ttttacctgt cgctttgact
     9661 gcttttacaa aatcttcgtt atcccaggcg ttaatattgc ccgggcgagc aatgtaaggt
     9721 gtatcgggga attgtgcttt cagttctgga actaatgggc cgttaggacc ggtttcaaaa
     9781 ctggtggtaa gaatagttgg taagttgaaa tacttagcca ggtcacccag cgccagcacg
     9841 ttatttttaa acttatcggg ttcgatatcc cgtacaaggg aaagtaaacc agcctggtga
     9901 tcaacaagca aaacggcagc atcattttta tcaagacgaa catacggttt ggtcatcctc
     9961 ttctcctttc gagagtcagt ggtacgtctg aaatcggtca gacgcgatgc attgctctga
    10021 aagcatagac gggaaatatg agtttgctgt gaccatgaaa tttttcgact gaacgaagcg
    10081 tatgaaattt gtgttagatc aataaaaaca atcagatgag attaatcatc tcttcagcat
    10141 tagcgttatg aatgagtaat ataaatcgat aataccgccg ctgcgatatg cgcgtatttt
    10201 cacccattga caatgttttt ggcggtggca tgatgcgcat gaaatttgaa cttcctcacg
    10261 gttttaattc atgtccacgt atacccggcc tgtcatgctt ttgctgtctg gcctgctttt
    10321 gttgactctg gcgatcgcgg tgttaaatac actcgtgccg ctttggctcg cccaggaaca
    10381 catgtccaca tggcaggtag gcgttgtcag ctcatcctat tttaccggca accttgtcgg
    10441 tacattgctg acagggtatg tcattaaacg cattggcttt aaccgcagct attatctgtc
    10501 ctccttcatt tttgctgctg gctgtgccgg ccttggcctg atgattggtt tctggagctg
    10561 gttggcgtgg cgttttgtcg cgggcgtcgg ctgtgccatg atttgggtgg ttgttgagag
    10621 cgcgctgatg tgcagtggga cgtcacgtaa ccgtgggcgt ttgcttgctg cgtatatgat
    10681 ggtttattac gtgggaacgt ttttaggcca gttactggtc agcaaagtct caaccgagct
    10741 gatgagcgta ttgccgtggg ttacaggttt gacattggca gggatcttac cgctgttgtt
    10801 tacgcgtgtc ctgaatcagc aggctgaaaa ccatgattcg acgtcaatta cggcaatgct
    10861 aaaactccgt caggcgcggc ttggcgtgaa tggctgcatt atctcaggaa tcgttctggg
    10921 atctctatat ggcctgatgc ctctgtacct caatcacaaa ggggtgagca attccagtat
    10981 tggtttctgg atggcggtac tggtcagtgc gggtatcctc ggacaatggc cgattggacg
    11041 tctggcggat aagtttggtc gactgctggt tttgcgtgtt caggtctttg tcgtcattct
    11101 cggcagtatt gcgatgctta gccaggcggc gatggcccca gcgttattca tcctcggtgc
    11161 cgctggcttt acgctatatc cggtggcgat ggcctgggct tgtgagaaag ttgaacatca
    11221 tcaactggtg gcgatgaacc aagccttact gttgagctat accgtgggaa gtctgcttgg
    11281 cccgtcattt accgctatgc taatgcagaa tttctccgat aatttattgt ttatcatgat
    11341 cgccagcgta tcgtttatct atttgctgat gctgcttcgc aacgccggtc atacgccgaa
    11401 acccgttgct cacgtgtaaa tgaattcaag caaagtgtga acttactgtt tcacactctg
    11461 cttttttgtt tcttctatct gacttgcttt tttccaaatt ttattcgttt aaaaataaaa
    11521 tgtgcagcag gttataattt tgcatttcgc tattaccgca cttcttattt gccgcgcata
    11581 atccctcgtt ttacagatgc ccctttaatt ttggcgaagg atttgtctat ggctgggaat
    11641 gttcaggaaa aacagttgcg atgg
//
LOCUS       DADLSA010000120        11582 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.120, whole genome shotgun sequence.
ACCESSION   DADLSA010000120 DADLSA010000000
VERSION     DADLSA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.120"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..1176
                     /locus_tag="JN691_003128"
     CDS             <1..1176
                     /locus_tag="JN691_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244503.1"
                     /translation="RLNQQHNEFMMVAKWCEWAIQQDGLQENLQKNLYEILEENQQNK
                     QSEIPQEDLKESLEEIKENILEENLPASRIENRAEALRRMKECLITRRSMLNLSNLGL
                     TSLPENLPPHLIEFYCSKNVLTALPKVMPKWLLVLDCTDNVLILLPKVQPSKLMVLKC
                     YENCIIWLPELSTNLRVINCSENFLQFLPPSMPQYLYKLSCAGNNINSIPDEMLENLT
                     RLKVFDCSSNDLISSPRLPPKLIIYYCGENKFKTVQVPQPQSLKVFDCNGNPWDKDNL
                     PTLLKAVEGLKKQQGLKDLLDFLHKEGQVDLEGLEELEDLMDLEFLDDPELLERVKVQ
                     EDLELLDQQLGLLSLERQQDSQPVNQQSEHEPESASRVKRDLSEVDSESTMKRKRFM"
     gene            complement(1344..2414)
                     /gene="dadX"
                     /locus_tag="JN691_003129"
     CDS             complement(1344..2414)
                     /gene="dadX"
                     /locus_tag="JN691_003129"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HAY7244504.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     LRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(2424..3722)
                     /locus_tag="JN691_003130"
     CDS             complement(2424..3722)
                     /locus_tag="JN691_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAY7244505.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLVRMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFCDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            4052..5584
                     /locus_tag="JN691_003131"
     CDS             4052..5584
                     /locus_tag="JN691_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAY7244506.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFLPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVQDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(5636..6355)
                     /gene="fadR"
                     /locus_tag="JN691_003132"
     CDS             complement(5636..6355)
                     /gene="fadR"
                     /locus_tag="JN691_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAY7244507.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            6576..8117
                     /gene="nhaB"
                     /locus_tag="JN691_003133"
     CDS             6576..8117
                     /gene="nhaB"
                     /locus_tag="JN691_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAY7244508.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            8263..8793
                     /gene="dsbB"
                     /locus_tag="JN691_003134"
     CDS             8263..8793
                     /gene="dsbB"
                     /locus_tag="JN691_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAY7244509.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(8839..10107)
                     /locus_tag="JN691_003135"
     CDS             complement(8839..10107)
                     /locus_tag="JN691_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAY7244510.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMFSPRYTTRSSDLLRVK"
     gene            complement(10107..10526)
                     /gene="umuD"
                     /locus_tag="JN691_003136"
     CDS             complement(10107..10526)
                     /gene="umuD"
                     /locus_tag="JN691_003136"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY7244511.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            10899..11582
                     /gene="hlyE"
                     /locus_tag="JN691_003137"
                     /pseudo
     CDS             10899..11582
                     /gene="hlyE"
                     /locus_tag="JN691_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309704.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
BASE COUNT         2898 a         2976 c         2822 g         2886 t
ORIGIN      
        1 cgcctgaatc agcaacataa cgagttcatg atggtcgcca aatggtgcga atgggctata
       61 cagcaggatg gtttgcaaga aaacctgcaa aaaaacctgt acgaaatcct ggaagaaaac
      121 cagcaaaaca aacagtcaga aattccccaa gaagacctga aagaaagtct ggaagaaatc
      181 aaggaaaata tcctggaaga aaacctgcca gcaagccgga ttgaaaaccg ggcagaagca
      241 ttgcgcagaa tgaaggaatg tctcattaca agacggagta tgctcaatct tagcaacctg
      301 ggattgactt cactccctga gaatttgcct ccacatctga ttgaatttta ctgcagtaaa
      361 aacgtgttga ccgcgttacc gaaggtgatg ccaaagtggc tgctggtgct tgactgtaca
      421 gataacgtct tgatcttgtt accgaaggtg cagccctcaa aactgatggt actgaagtgc
      481 tatgaaaact gtattatctg gctgcctgag ctgtcgacta acctgagagt gattaactgt
      541 tctgaaaact tcttgcaatt tttaccgccg tcgatgcccc agtacctgta taaactatcc
      601 tgtgccggca acaatattaa ctcaatacct gatgagatgc tggagaactt gactcgcctg
      661 aaagtatttg actgttctag taacgatttg atctcttcac cacggctgcc gcccaaacta
      721 ataatttact actgtggaga aaacaagttt aaaacggtac aggtgccgca gccccagagc
      781 ctgaaggtgt ttgactgtaa tggtaacccg tgggacaaag acaatttacc gacgctgctc
      841 aaagccgtcg agggcctgaa aaaacagcag ggcctgaagg accttttgga ctttttgcac
      901 aaggaaggcc aggttgacct ggaaggactc gaggagctgg aggaccttat ggatctggag
      961 ttcctggatg acccggaact cctggagcgc gtgaaggtac aggaggacct ggagctcctg
     1021 gatcaacagt tgggcctgtt gagtctggaa agacaacagg actcgcagcc tgttaatcaa
     1081 caatctgaac atgaacccga atctgcatca agggtgaagc gtgatttatc tgaagtcgac
     1141 tccgagtcaa caatgaagcg taagcgtttt atgtagaacg cctgtgcggt gagttcaggt
     1201 tccggggaaa tagtgtccat tcttttaatg gacactatca tgggcaatgg ctctgtcaca
     1261 ttaacaacta cagttgctga ccagccggat ggatgatttt ttcgcaacca gaagacgctg
     1321 cctccgatcc ggcttacaac aagttacact gtcacaaccg ggacgcgtaa cgccagcgcg
     1381 cacatcaact catagcccac cgttccggca gcggcggcga catcatcaat tttgatctcc
     1441 ttgccccaca gctcaaccgg cgtaccaatc cccgcctgcg ggcaaggcgt taaatcaacc
     1501 gccagcatat ccatcgagac ggtccccacc gtcatggtgc gcacgccgtc cactaaaaca
     1561 ggagtaccgg taggcgcatg gcgcagataa ccgtcggcgt atcctgcggc gacaatgccg
     1621 attcgctgtt catcgcgcgc agtatagcga ccgccgtagc ccacacgctc gcccgctttt
     1681 agcgtctgga caccaataat ctcactgctt agcgtcatca ccggacgtaa tccggtattg
     1741 gcgatatcac gccactgacc ggacggcgaa gcgccataca aaataatgcc aggccgaacc
     1801 cagtcaaaat gcgcttccgg gtgccacaga gtcgccgccg aattggacaa cgaacgccga
     1861 cactccagcc cctccgccgc ctgctcaata cgcgccatcg cgccggaaat tccatcagga
     1921 tgttccgctt cagcaaaatg cgacatcagg gtcatttcgc caacattcgc cattgcccgc
     1981 aactgctgcc agacggtaag cacgcgatcg ggctggaagc ccaaccgatt catcccactg
     2041 ttcactttaa gataaatatc caacggtgct tttagccgcg cattttgcag tgctttgagc
     2101 tgccagttgc tgtgtacgca ggtggtcagg cggtgctggt cataaatctc cagatcctga
     2161 gcatggaaaa atccttccag catcaggatc ggccccttcc agccgcgctc acgtaacgtt
     2221 attgcctctt ccaggttaag taatgcaaag ccatcggtgg ccccgagcgc gctccagata
     2281 cgctcaatac catgcccgta agcgttcgct tttaccaccg accagacgcg cgcgtgcggc
     2341 gcggcctggc ggacaatgga cagattctgt tttaatgcct gcagatcgag gctggcctgt
     2401 atcggacggg tcatctcgtt tccttagctg tgtgcgccat gtaaatggcc cggacgtgat
     2461 ggcgtaaatc cacggctgta gcgcgctacg cttagatcct catatgggat cgctggcgtg
     2521 cgaccagaga gcagatcgct taacaactga ccggaaccgc aagccatcgt ccagccgagc
     2581 gtgccgtgac cggtattcag ccacagattt ttaaagcgtg tacgcccgac aaccggcgta
     2641 ccgtctggcg tcatcgggcg caaacccgtc cagaaagtcg cctgctcgac atgaccgccg
     2701 cgtggataga gatcgcgaac caccatttcc agcgtttcac gacgcggttg caacagctcg
     2761 gtgttaaaac caacaatttc cgccattccg ccaacacgaa tgcggttatc gaaacgagta
     2821 atggcgattt tgtaggtttc atcaagaatg gtggatactg gcgcaccatc ttcctgcgca
     2881 attggaatag tcagcgagta gcctttcagc ggatacaccg gaatatcaac aatgcctttg
     2941 agcatcgccg tcgagtaaga accaaacgcc atcacatacg catcggcctt aatcacttca
     3001 tcgcaaaact tcacgccgta gatttgctcg ccgtcgcaaa gcagttggtc aacgggcgta
     3061 ttaaagcgga atttaacccc cgcctgctct gccatccgca ccagattctg ggtaaacagc
     3121 tggcagtctc cggtttcatc attgggtaac tgcaggccgc ccgtcagttt gtgcgctact
     3181 tctgccagcg cgggatccac ttccgccagg cggctggatt ccagcagctg atacggtacg
     3241 ccggcatctt ccagcacggc gatatcgcgg gtcgcatttt catactgttg ttcggtacgg
     3301 aacagttgca gcgtcccacc ctgacgccct tcatactgaa tattggtttc ggcgcgcaat
     3361 gctttcaggc aatcacggct gtattccgcc agacgcacca tccgcccttt gttttccatg
     3421 tagtggctgg tgtcgcagtt acgtaacatt tgccacatcc atttcaactg aaactgcgta
     3481 ccgtcgagac gaaccgccag cggcgcatgg cgctggaaca tccatttaat cgcttttaaa
     3541 ggcacacctg gtgctgccca cggtgcagca tatccggggg agatttgccc ggcattcgcg
     3601 gcactggttt ccagggcggc ccccggctcc cgatcaatga cggtgacctc atgtcctgcc
     3661 tgatttaagt accaggcgct ggcaacgcct accacaccac ttcccagtat gacaactcgc
     3721 atagccactc cgataacggt agatacagta aaagaataat aatctaatta catcttgata
     3781 actcagttga aaatattatt caacatacgg cttttttatg gtgagctgac tcacatctcc
     3841 ctgactctgc ggcaatatgg cgtctttagt atctcctgct gaacgccctt tttatccagc
     3901 ataaaattcc gttcagaagc ggattagtgg cactctgata ataagaaatc gcaaagaaaa
     3961 aaattctccg gcgacgcggt ttttaacagg tgttctatgc ttgaaatgag gtgctccaca
     4021 cagagagcgc cgacaacaat gaggtgcgcg tatggcgacg atcgattcta tgaataagga
     4081 caccacacgt ttgagcgatg gacccgactg gacgttcgac ctgctggatg tttatctggc
     4141 agagatagac cgggtggcga aactctaccg gctggatacc tacccgcacc agattgaagt
     4201 gataacctca gaacagatga tggatgccta ttccagcgtc ggcatgccaa ttaactatcc
     4261 gcactggtca ttcggtaaaa agtttatcga gactgaacgg ctgtataagc acggtcagca
     4321 agggctggcc tatgaaatcg tcattaactc taacccgtgt atcgcttact tgatggaaga
     4381 gaacaccatt accatgcagg cgctggtgat ggcacacgcc tgctatgggc ataactcttt
     4441 ctttaaaaac aattgcttat tccgcagttg gaccgacgcc agttcgattg tcgattatct
     4501 gatttttgcc cgtaaatata ttaccgagtg cgaagaacgt tatggcgttg atgaagtaga
     4561 acggcttctg gactcgtgcc acgcgctgat gaactacggc gtggaccggt acaaacgccc
     4621 gcaaaaaatc tcgctgcaag aagagaaagc ccggcagaaa agtcgcgaag agtatctaca
     4681 aagtcaggtc aatatgctct ggcgtaccct gccgaagcgc gaggaagaga aaacggttgc
     4741 tgaagcgcgc cgctatccgt ccgaaccaca agaaaacctg ctctatttta tggagaaaaa
     4801 tgcgccactg ctggaatcat ggcagcgtga aatcctgcgt attgtgcgta aggtgagcca
     4861 gtatttttat ccgcaaaaac agactcaggt gatgaacgaa ggctgggcga ccttctggca
     4921 ctataccatc cttaaccatc tgtatgatga agggaaagta acggaacgtt ttatgctgga
     4981 gtttttgcac agccacacca atgtggtctt cctgcccccc tataacagcc cgtggtacag
     5041 cggcatcaac ccgtatgccc tcgggttcgc catgttccag gatattaaac ggatttgtca
     5101 gtcgccaacg gaagaagaca aatactggtt cccggatatc gccggttccg actggctgga
     5161 aacgctgcat ttcgcgatgc gtgatttcaa agatgagagt tttatcagcc agtttctgtc
     5221 accgaaagtg atgcgtgatt tccgcttctt caccgtgcag gatgatgatc ggcataatta
     5281 tctggagatt tccgctattc ataatgaaga aggttatcgg gagatccgta accggttatc
     5341 gtcgcaatat aacttaagta atctggagcc gaatattcag atctggaacg tggatttgcg
     5401 cggcgaccgt tcgctgacgc tgcgttatat tccacataat cgcgcaccgc tggatcgggg
     5461 gcgcaaagaa gtcctgaagc atgtgcatcg cctgtgggga tttgatgtga tgctcgaaca
     5521 gcaaaacgaa gacggcagca tcgagttgct ggaacgttgc ccgccaagaa tgggaaatct
     5581 gtaaaaacaa caaaaaaccc ctcgtttgag gggtttgctc tttaaacgga agggattatc
     5641 gcccctgaat ggctaaatca cccggcagat ttttctgcat ccggtgccaa atctcgccac
     5701 tctcatgccc atagcgacgc actgtttcgt acacctgatc gtgcgcgcct tcactgcaca
     5761 acgccgacag tttgtggtag aagcccagcg ccagactgcg cgcttccgga ttggcgaaat
     5821 agtgacgacc aatgcgcgta tacagccctt tcatcccgtt aaggatcaga ccgtaaatcg
     5881 ggttgccgga agcaaacgcc aggccgcgga atatgttgta atccagctcg gcaaaggcat
     5941 cggcgtgatc ggccacttca ttagcggtag ccagcacttc ctgcgcttta tcgggatgct
     6001 gacgaaacgc ggtgcgaata aaaatagtgg aaatattggt acgcaccgac agcaaattat
     6061 caataagctg cggcacactt tcgtgatcca gtcgcgccag tgtttcaagg atatttaaac
     6121 cggaagtttc ccagaaatta ttcaccttcg tcggcttgcc atgttgaatg gtcaaccagc
     6181 catctcgtgc cagacgctgt aacacttcac gtaacgtagt acgcgttacg ccaattaatt
     6241 ctgaaagttc acgttctgcg ggcaaaatag tcccgggagg gaagcggtta ttccagatac
     6301 tttcaataat gtactcttcc gcgaaacccg ccgggctttg cgccttaatg accatagtga
     6361 gatttccata acacagcaaa acaaagttgg actcatcata ccagagggct aactacgctg
     6421 atagcagaca aaaagaagaa aaagggatca aggcttcatt ttccgcatct ctttcccctt
     6481 gactatcaag acttgcctca ctcctgcctg accgttaagc tttgcgttta aacataaaaa
     6541 catcattgat tatttgtaag gtaagggaaa ccatcatgga gatctcctgg ggccgcgcgc
     6601 tatggcgcaa ttttttgggc cagtcccccg actggtacaa actcgccctc atcattttct
     6661 taatcgtaaa cccgttaatt ttcctcatca gccctttcgt cgcgggttgg ttgctggtag
     6721 cggaatttat tttcactctg gcgatggccc tgaaatgcta cccgctactc cccggtggtc
     6781 tgttggctat cgaagcggta ttcatcggca tgaccagcgc ggaacacgtc cgtgaagagg
     6841 tggcggcaaa tcttgaagtc ttgctgttat tgatgtttat ggtggcgggt atctatttta
     6901 tgaaacagct gttgctgttc atatttaccc gtttgctgtt aagcattcgc tccaaaatgc
     6961 tgctgtcgct ctctttttgc gtggcggcag cgttcctctc cgcgttcctc gatgccttaa
     7021 ccgtcgtggc ggtggtgatc agcgttgcag tcggttttta tggtatttat catcgcgtag
     7081 cctcttcccg taccgaagac accgacctgc aagacgatag tcatatcgac aagcattaca
     7141 aagtggttct ggaacagttc cgtggctttc tgcgtagcct gatgatgcat gccggtgtcg
     7201 gcaccgcatt aggcggcgta atgaccatgg tgggcgaacc acagaacctg atcatcgcta
     7261 aagcggctgg ctggcatttt ggcgatttct tcctgcgcat gtcgccggtg accgttccgg
     7321 ttctgatttg tggcctgtta acctgcctgc tggtagagaa gctgcgttgg tttggctacg
     7381 gtgaaacgct gccggagaaa gtccgcgaag tgttgcaaca gtttgacgat caaagccgcc
     7441 accagcgtac ccgtcaggat aaaatccgtc tgattgtcca ggcgattatt ggcgtctggc
     7501 tggtgactgc gctggcgttg catctggcgg aagttggctt gattggtttg tcagtcatta
     7561 ttctggcaac atcattgacc ggtgtcaccg atgaacatgc tatcggtaaa gccttcaccg
     7621 aatctctgcc attcaccgca ctgttgacgg tgtttttctc ggtagtcgcg gtgattatcg
     7681 accaacaact gttttcgccg attattcagt ttgtgttgca ggcatctgaa catgctcagc
     7741 tgtcgctgtt ctatattttc aacggtctgc tgtcatccat ttcggataac gtcttcgtgg
     7801 ggacgattta tatcaacgaa gcgaaagcgg caatggaaag tggcgctatc acgttgaagc
     7861 aatacgagct gctggcggtc gccattaata ctggtaccaa tctgccctct gtcgctacgc
     7921 cgaacggtca ggctgcgttc ctgttcctgc tgacctctgc actcgcgcca ttgattcgcc
     7981 tctcttatgg ccgcatggtg tggatggccc tgccttacac cctcgtactg acactcgtcg
     8041 gcttgctctg cgtcgagttt acgcttgccc ctgtaaccga atggtttatg caaatgggct
     8101 ggatagcaac gctttgataa caacttaccg ggcatataaa tgcccggttt gccttttcgc
     8161 ccgataattg tccaattgcg tcctattttt gctgcctcct ggtggcgcag cgaatgaatt
     8221 ggtttaaact gcgcactcta tgcatattgc agggaaatga ttatgttgcg atttttgaac
     8281 caatgttcac aaggccgggg cgcgtggctg ttgatggcgt ttactgctct ggcactggaa
     8341 ctgacggcgc tgtggttcca gcatgtgatg ttactgaaac cttgcgtgct ctgtatttat
     8401 gaacgctgcg cgttattcgg cgttctgggt gctgcgctga ttggcgcgat cgccccgaaa
     8461 actccgctgc gttatgtagc gatggttatc tggttgtata gtgcgttccg cggtgtgcag
     8521 ttaacttacg agcacaccat gcttcagctc tatccttcgc catttgccac ctgtgatttt
     8581 atggttcgtt tcccggaatg gctgccgctg gataaatggg tgccgcaagt gtttgtcgcc
     8641 tctggcgatt gcgccgagcg tcagtgggat tttttaggta tggaaatgcc gcagtggctg
     8701 ctcggtattt ttatcgctta cctgattgtc gcggtgctgg tggtgatttc ccagccgttt
     8761 aaagcgaaaa aacgtgatct gttcggtcgc taatccattc ggcgctcctg cgggagcgct
     8821 tttttcctgc cgctatattt atttgacccg cagtaaatca gaacttcgcg ttgtataacg
     8881 tggtgaaaac atagctcgct tcatctgcca ttgttgttgg atcccctgcc cggcaaaata
     8941 gagtgttcct ctgccctctt tagcattcag agtatccatt accgccatca attgctcact
     9001 cccggggcgc ggtgcgttgt catcgaataa attgagctgc gcgactccct gactgaagaa
     9061 atcccccagc atcacgcccg ctttttggta gcgatggccc gcttgccaga tggcatccag
     9121 agatcgcgta gcagcgttaa tgatatccct gctgtcctga gtgggcgtca gcagttttac
     9181 cgacgcgcta ttgccgtaat aaggttcatt gagcgcaaat ggtgacgtct taataaatgt
     9241 ggagataaac cgacaatatt gatgctcact gcgaagtttt tccgccgccc gggcagcgta
     9301 actacaaatg gcctgccgca tcgacgtata atccgtgatg cgttcaccaa acgagcggga
     9361 acagataatt tcctgcttcg tcggtgcaaa ctcttccagt tgcaaacagg gttcgccgcg
     9421 cagttcacgc accgttcttt cgagcatgac attaaaatgt ttacggataa accggatatc
     9481 tgtatccgcc aaatcgagaa cggttttgat ccccatcgcg tccagttttt tgctgatccg
     9541 ccgtccaatc ccccagacgt catccacggg gagagcagac attaatttac gctggcgttc
     9601 cagatttgat aaatccacca ccccacccgt ctgccgctgc catttttttg ccgcatgatt
     9661 ggcaagctta gccaacgttt tggtctgggc gatccccaca ccaacagtaa gatgggtacg
     9721 ttgtagcacc gttgcgcgaa tttctctgcc aaaatcagtc agatcgcgac aattacgcac
     9781 acctgtcaga tcgcagaatg cttcatcaat actgtaaatc tcgacgcggg gcgatagttc
     9841 ttccagcgtc gacatcaccc gattgctcat gtctgcgtaa agctcataat tgctgctaaa
     9901 gcaaaccaca ccacagcgac gaaacagatc tttttgtttg aaccagggat cgcccatttt
     9961 aacgcccagc gcctttgcct cagcgtttcg ggcgataacg caaccgtcat tattcgatag
    10021 cacaaccacc ggtttacccc ataaatcagg gcgaaacacc gtctcacagc tggcataaaa
    10081 cgcgtttaca tcacagaggg caaacatcag cgcatcgcct taacgacgtg gatcaccaca
    10141 ccaaagacat ccagcgtatc ttcgctactg atggtaatgg gcgagtacgc actgttcatg
    10201 ggaataagct gtaccgtcgg gcgtagttgc aattttttca ccgtaaactc gccgtcaaca
    10261 gcagcgatga caatatcacc atggctggcg gtaatagcgc tatcgacaat cagtaaatca
    10321 ccgtcactaa ttccaccatc aatcatagaa tcaccacttg ctttgacgaa gtaagtcgcg
    10381 ctgggatgct ggatcaacag ttgattcaga tcgatgcgct gttcaacgta atctgctgcc
    10441 ggtgaaggaa agccacactg aacaagatcg ctaaatagcg gaaaagtcac aatttcgcgg
    10501 agatccgcag gcttgataaa caacataata atctgcctga agttatactg tttttatata
    10561 cagtagtctg ttcttgccag cagatcaata ctgattcagg ctatcaatat ttgtcgctgc
    10621 atagactgct gatttttcgt tctcttatcc tgtgctcacg tggccttctg gcgacgacgc
    10681 tcatccagca gaaatgaaaa atatcacccg gcaaaaaaat agaatagaag cattcgccat
    10741 aatgacatta aacattgttt gatatttatc atattaatag aaataaagac attgacgcat
    10801 cccgcccggc taactatgaa ttagatgaag taaaatttat taatagttgt aaaacaggag
    10861 tttcattaca atttatatat ttaaagaggc gaatgattat gactgaaatc gttgcagata
    10921 aaacggtaga agtagttaaa aacgcaatcg aaaccgcaga tggagcatta gatctttata
    10981 ataaatatct cgatcaggtc atcccctggc agacctttga tgaaaccata aaagagttaa
    11041 gtcgctttaa acaggagtat tcacaggcag cctccgtttt agtcggcgat attaaaacct
    11101 tacttatgga tagccaggat aagtattttg aagcaaccca aacagtgtat gaatggtgtg
    11161 gtgttgcgac gcaattgctc gcagcgtata ttttgctatt tgatgagtac aatgagaaga
    11221 aagcatccgc ccctcattaa ggtactggat gacggcatca cgaagctgaa tgaagcgcaa
    11281 aaatccctgc tggtaagctc acaaagtttc aacaacgctt ccgggaaact gctggcgtta
    11341 gatagccagt taaccaatga tttttcagaa aaaagcagct atttccagtc acaggtagat
    11401 aaaatcagga aggaagcgta tgccggtgcc gcagccggtg tcgtcgccgg tccatttggt
    11461 ttaatcattt cctattctat tgctgcgggc gtagttgaag ggaaactgat tccagaattg
    11521 aagaacaagt taaaatctgt gcagagtttc tttaccaccc tgtctaacac ggttaaacaa
    11581 gc
//
LOCUS       DADLSA010000121        11541 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.121, whole genome shotgun sequence.
ACCESSION   DADLSA010000121 DADLSA010000000
VERSION     DADLSA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.121"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(520..1860)
                     /gene="fadL"
                     /locus_tag="JN691_003138"
     CDS             complement(520..1860)
                     /gene="fadL"
                     /locus_tag="JN691_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAY7244512.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            2232..2516
                     /locus_tag="JN691_003139"
     CDS             2232..2516
                     /locus_tag="JN691_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAY7244513.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2696..4006
                     /gene="fadI"
                     /locus_tag="JN691_003140"
     CDS             2696..4006
                     /gene="fadI"
                     /locus_tag="JN691_003140"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAY7244514.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVLLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            4006..6150
                     /gene="fadJ"
                     /locus_tag="JN691_003141"
     CDS             4006..6150
                     /gene="fadJ"
                     /locus_tag="JN691_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAY7244515.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPFIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6353..6838
                     /gene="sixA"
                     /locus_tag="JN691_003142"
     CDS             6353..6838
                     /gene="sixA"
                     /locus_tag="JN691_003142"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAY7244516.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7521..8084
                     /locus_tag="JN691_003143"
     CDS             7521..8084
                     /locus_tag="JN691_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7244517.1"
                     /translation="MSKFVKTAIAVAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            8166..10807
                     /gene="yfcU"
                     /locus_tag="JN691_003144"
                     /pseudo
     CDS             8166..10807
                     /gene="yfcU"
                     /locus_tag="JN691_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            10827..>11541
                     /locus_tag="JN691_003145"
     CDS             10827..>11541
                     /locus_tag="JN691_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7244518.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTSYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGN"
BASE COUNT         2956 a         2675 c         2968 g         2942 t
ORIGIN      
        1 aagtagatgt ttaatacagg tgcttattcc ttccttacca ttaaaaatgg tttttgaact
       61 cttgtactaa tccattaatt tcatcattgt tattttcatt gatggattct gggtaagctg
      121 cgctggtatt gttgattgaa atagatggta gcatgatttt attccatgtt gattgatttg
      181 ttacaaatat atattaccta atgaaataga ctatcaaaaa acgctcattt atttagaacc
      241 tatctttaat ttgagtgtat aagcgcacaa tagccagggg ggttgagtat cctggaaatg
      301 cagaaaagta aactctttga gaaggacgag cagaacgctt gaaaatatgc atttcccttc
      361 aggtttgtct gtggatacca cttactgatt tgaaagggca agctgccatc aggctgtttg
      421 caaaactcag gaggcaagca atgaggaaac tactttcggt gaagtggggg agttaaagtc
      481 acctgcatag caggtgactt tatccaggcg aacgcgttat cagaacgcgt agttaaagtt
      541 agtaccgaac agccaggctt taccttcaga ctcgaactgg tatgggcctt cgttaatttt
      601 cacgctctga ccgtgcatat aagaaacacc aacgtcgact gaagcatctt tattaaatgc
      661 gtaagtcgta cctgcactca gccagaaacg gtcctggtcc ggaatggaga tagaacgatt
      721 ctgagccgga actgggctgt catcaaaggc gataccggta cggaaggtcc agttatcatc
      781 gtagtaataa gtggtaccca acgcgatgcg gtaagcatct ttaaagcctt catgtttctg
      841 gaacagcgtg tcgccactgg ttgaggtcgc tttcagctgc tggaactgac tccagctggt
      901 gtaagccagg ctatagtgaa tcgcccactg tggatcaaca cggttataac ctgacacttc
      961 ccacatttca ggcaggttca gcgtcagata acccgattgc gttgcgccac ctgtcgcggt
     1021 aggaattggc aaaccgtagt tattaaacgc acgattaaga tcgctgctgt agttaccttt
     1081 gaagtcaatt ttcacttcag aacggtaggt cagtgcatag cggttatttt tatccagttc
     1141 atacaggatt ccggcgttcc agccaaagcc ccactggtta ccgttcagat gagcgatttt
     1201 ggtattactg tcgataccgt tggcggtagc tgccaatgct tgcccttgag gagtttgccc
     1261 ggcaggagat tgcataatct gaccagcaac caactgcccc agatcgcctg cgaaacgttc
     1321 aattttcgcg cgagcgtaga tggcgttgaa accaagacca aagctccatg cattatttaa
     1381 gcgatacgca ccgcttaagt tcaggttcat ggtttcaagg tcagttgtac ccccgacaga
     1441 gccgcctgca taagtatcat taaactctgt tgccagacca tagttagagg taatagaagc
     1501 gccccaacca aattggtcgt taatcggtgc aacaaagtgc atgttcggaa cccatgccgt
     1561 aggcgcgatg ttatcggctt tcaggctacg accagacgga gacgttccgc tgatattcac
     1621 atccgggtca atataaaccg cacccgcaga aaatgtcggg cggtcaaaca tggtaatcaa
     1681 tgcggggtta cggctaacgt tacctgcatc atcggcaatt gcgccttccc ctgaataagc
     1741 ccggcccagg ccagaggaag aaaattcgtt taactgaaag cctgccgacc aggcctgggt
     1801 ggagataagt gccactgcga ctgcgagagc agactttgta aacagggttt tctggctcat
     1861 gaccataacc tcaatgattt atttttatac aaactatgtt acgtgctgta acaggagcgc
     1921 gaagtgtagg gtctgaggta catcttagaa atcagaccag tggcgagagt ataggtcgga
     1981 ccagctagaa tattgcaagt atgtttccat cttttttcaa atatgatcta agaaacgcga
     2041 tccagctggc aaaatttgga ggttgcgcac tcgcggattc gggtgaattt tgttttagat
     2101 catttttaag tgtgatttcg gtcacttatc cgattttgca gaattaacaa ctggagcagc
     2161 actttccgga agcgtaaaat atccacaacg tttatctggc accttgggtg cacatacaga
     2221 ggaaatcaac gatgagtaaa tgtagtgctg atgaaacccc ggtttgctgc tgtatggatg
     2281 ttggcaccat tatggacaac tccgactgca ccgcgtctta cagccgcgtg tttgcaaacc
     2341 gcgcagaagc agaacaaacg ctggcggcgc tgactgaaaa agcccgtagc gtggaatccg
     2401 aaccgtgcaa aatctccccg actttcactg aagagtctga cggtgtgcgc ttggatatcg
     2461 atttcacttt tgcctgcgaa gctgaaatgc tgatcttcca gctgggtctg cgttaattcc
     2521 tctcgggtac gtacgcccct gtcagttgct ggcaggggcg ttttcttttc tttcttctgt
     2581 cttcactgtg tcatagctcc cactttcctt tttctccgct tggctaaatg taaaaaaatg
     2641 gttaagacta tgatcaggtc agaccacttt atttattttt ttacagggga gtgttatggg
     2701 tcaggtttta ccactggtta cccgccaggg cgatcgtatc gccattgtta gcggtttacg
     2761 tacgcctttt gcccgccagg cgacggcttt tcatggcatt cccgcggttg atttagggaa
     2821 gatggtggta ggcgaactgc tggcacgcag cgagatcccc gctgaagtga ttgaacagct
     2881 ggtctttggt caggtcgtac aaatgcctga agcccccaac attgcgcgtg aaattgttct
     2941 cggtaccgga atgaatgtgc ataccgatgc ttacagcgtc agccgcgctt gcgctaccag
     3001 tttccaggcg gttgcaaacg tcgcagaaag cctgatggcg ggaactatcc gagcggggat
     3061 tgccggtggg gcagattcct cttctgtatt gccaattggc gtcagtaaaa aactggcgca
     3121 cgtgctggtt gatgtcaaca aagctcgaac catgagccag cgcctgaaac tcttctctcg
     3181 cctgcgtttg cgcgacttaa tgcccgtacc gcctgcggta gcagaatatt ctactggctt
     3241 gcggatgggc gacaccgccg aacaaatggc gaaaacctac ggcatcaccc gagaacagca
     3301 agatgcatta gcgcaccgtt cgcatcagcg tgccgctcag gcatggtcag acggaaaact
     3361 caaagaagag gtgatgactg cctttatccc tccttataaa caaccgcttg tcgaagacaa
     3421 caatattcgc ggtaattcct cgcttgccga ttacgcaaag ctgcgcccgg cgtttgatcg
     3481 caaacacgga acggtaacgg cggcaaacag tacgccgctg accgatggcg cggcagcggt
     3541 gatcctgatg actgaatccc gggcgaaaga attagggctg gtgctgctgg ggtatctgcg
     3601 cagctacgca tttactgcga ttgatgtctg gcaggacatg ttgctcggtc cagcctggtc
     3661 aacaccgctg gcgctggaac gtgccggttt gacgatgagc gaactgacat tgatcgatat
     3721 gcacgaagcc tttgcagctc agacactggc gaatattcag ttgctgggta gtgaacgttt
     3781 tgctcgtgaa gtactggggc gtgcacatgc cactggcgaa gtggacgata gcaaatttaa
     3841 cgtgcttggc ggttcgattg cttacggaca tcccttcgcg gcgaccggcg cgcggatgat
     3901 tacccagaca ttgcatgaac ttcgccgtcg cggcggtgga tttggtttag ttaccgcctg
     3961 tgctgccggt gggcttggcg cggcaatggt tctggaggcg gaataatgga aatggcatca
     4021 gcgtttactc ttaatgttcg tctggacaac attgctatca tcaccatcga cgtaccggat
     4081 gagaaaatga ataccctgaa ggcggaattt gcctcgcagg tgcgcgccat tattaagcaa
     4141 ctccgtgaaa acaaagagtt gcgcggcgtg gtgtttatct ccgcgaaacc ggacaatttt
     4201 atcgccggag cagacatcaa catgattggc aactgcaaaa cggcgcaaga agcggaagca
     4261 ctggcgcggc agggccaaca gttgatggcg gagattcatg ctttgcccat tccggttatc
     4321 gcggctattc atggcgcttg cctgggcggt gggctggagc tggcgctggc gtgccacggt
     4381 cgcgtttgta ctgacgatcc taaaacggtg ctcggtttgc ctgaagtaca acttggattg
     4441 ttacccggtt caggcggcac ccagcgttta ccgcgtctga taggcgtcag tacagcatta
     4501 gagatgattc tcaccggaaa acaacttcgg gcgaaacagg cattaaagct ggggctggtg
     4561 gatgacgttg ttccgcactc cattttgctg gaagccgctg ttgagctggc gaagaaggat
     4621 cgcccatctt cccgccctct acctgtacgc gagcgtattc tggcggggcc gttaggtcgt
     4681 gcgctgctgt tcaaaatggt cggcaagaaa acagaacaca aaactcaagg caattatccg
     4741 gcgacagaac gcatcttgga ggttgttgaa acgggattag cgcagggcac cagcagcggc
     4801 tatgacgctg aagctcgcgc ctttggcgaa ctggcgatga cgccgcaatc gcaagctttg
     4861 cgtagtatct tttttgccag tacggacgtg aagaaagatc ccggtagtga tgcaccgcct
     4921 gcgccattaa acagcgtggg aattttaggt ggtggcttga tgggcggcgg tattgcttat
     4981 gtcactgctt gtaaagcggg gcttccggtc agaattaaag atatcaaccc gcagggcata
     5041 aatcatgcgc tgaagtacag ttgggatcag ctggagggca aagttcgccg tcgtcatctc
     5101 aaagccagcg aacgtgacaa acagctggca ttaatctccg gaacgacgga ctatcgcggc
     5161 tttgcccatc gcgatctgat tattgaagcg gtgtttgaaa atctcgaatt aaaacaacag
     5221 atggtggcgg aagtggagca aaattgcgcc gctcatacca tttttgcttc gaacacgtca
     5281 tctttaccga ttggtgatat cgccgctcac gccgcgcgac ctgagcaagt tattggcctg
     5341 catttcttca gtccagtgga aaaaatgccg ctggtagaga ttattcctca tgcgggtaca
     5401 tcggcgcaaa ccatcgccac tacggtaaaa ctggcgaaaa aacagggcaa aacgcccatt
     5461 gtcgtgcgtg acaaagccgg gttttacgtc aatcgcatct tagcgcctta cattaatgaa
     5521 gctatccgca tgctgaccga aggtgaacgg gtagagcaca ttgatgccgc gctagtgaaa
     5581 tttggttttc cggtaggccc aatccaactt ttggatgagg taggaatcga caccgggact
     5641 aaaattattc ctgtactgga agccgcttat ggagaacgtt ttagcgcgcc tgcaaatgtt
     5701 gtttcttcaa ttttgaacga cgatcgcaaa ggcagaaaaa atggtcgggg tttctatctt
     5761 tatggtcaga aagggcgtaa aagcaaaaaa caggtcgatc ctgccattta cccgttcatt
     5821 ggcgcacaag ggcaggggcg actctccgca ccgcaggttg ctgaacggtg tgtgatgttg
     5881 atgctgaatg aagcagtacg ttgtgttgat gagcaggtta tccgtagcgt gcgtgacggg
     5941 gatattggtg cggtatttgg cattggtttt ccgccatttc tcggtggacc gttccgctat
     6001 atcgattctc tcggcgcggg cgaagtggtt gcaataatgc aacgactggc cacgcagtat
     6061 ggttcccgtt ttaccccttg cgagcgtttg gtcgagatgg gcgcgcgtgg ggaaagtttt
     6121 tggaaaacaa ctgcaactga cctgcaataa gaaggtcaaa gctatatgaa tccgcgctga
     6181 atggcggagt gttggtcaaa atgtaaacgc atattgacta tacttacgcc attgaggtaa
     6241 aaaacagcgt ttcattcggt gaatggataa ggcacaatgc cggccaccgt tttctttctc
     6301 tggtttcaga tgaaagaaaa cgggcgaatc tggttaacaa aagcggtgca atatgcaagt
     6361 ttttatcatg cgtcacggcg acgcagccct cgatgccgcc agtgattcgg ttcgtcctct
     6421 gaccactaat ggttgtgacg aatctcgcct gatggcgaac tggctgaaag gtcaaaaagt
     6481 ggaaatcgaa cgcgttctgg tgagcccgtt cctgcgagcc gagcaaacac tggaagaagt
     6541 aggggattgt ctgaacctgc cttccagtgc agaagttctg ccggagttaa cgccctgcgg
     6601 cgatgttggt ctggtcagtg cctatttgca ggcgttgacc aatgaaggtg ttgcttcggt
     6661 actggtgatc tctcacttac cgttagtcgg gtatctggtt gccgagttat gtccgggcga
     6721 aacgccgcct atgtttacca cctcggccat tgccagcgta acgcttgatg aaagcggaaa
     6781 cggtacattc aactggcaga tgagcccgtg taatctgaag atggcaaaag ctatctgatg
     6841 tgttccaaat tatgcccaat gaggtagatt cattgggcat aattgctcat taggcagcgg
     6901 atatctgtca acctcactac tattgaagac tctccttatc tataaataaa acactttcta
     6961 cattcattcg gaaattattc ttctgcgcgt atcttgattt tgcccaaagt tacatgttga
     7021 aatgaataat gtctgtttga cactcattgt catttattaa tacagataat agagaaaata
     7081 gagttgaata atcaggcagg atgtaattga aacttgatta aattctggct atagtgttta
     7141 tatattatat tgtcaaaagt ggataaatgc caatcgcatt atatgttcat tgtttgtttt
     7201 ttaagctgtt gatattcatt attgatatac tttctatttt ttctaaatac ttattccgta
     7261 ttttttaagg atgtccttaa gataaccttt cttctgtcaa tatattaatt aaatctacat
     7321 ttactcttta gtaaaattaa attggctcag gggtgtggaa acatcactat gcggcccaca
     7381 ccaacgaata caattttcta cgacctgttc tttttgggtc gcatgccgta ttcaatggct
     7441 gggtataaac attggctatt gactgaatca atgtattgca atgtttatat ttttaattat
     7501 ttatagttta aggacaaata atgagtaagt ttgttaaaac agctattgct gtggcaatgg
     7561 tgatgggcgt gttcacctct acggcaacaa tcgctgcggg taacaacggt actgcacgtt
     7621 tttacggcac cattgaagac tcagtgtgtt ctatcgttcc ggacgatcac aaactggaag
     7681 ttgatatggg tgatatcggt gccgaaaaac tgaaaaataa cggcaccacc acgccgaaaa
     7741 gtttccagat tcgtctgcaa gattgcgtat ttgatactca ggaaacaatg accactacct
     7801 ttactggtac cgtttcttct gcaaatagcg gcaattatta caccattttc aataccgata
     7861 ccggtgcggc atttaacaat gttagcctgg cgatcggtga ctctctgggt acctcttaca
     7921 aaagcggcat gggtattgac cagaaaatag tgaaagacac ttctaccaac aaaggcaaag
     7981 cgaagcagac actgaacttt aaagcctggc tggtcggcgc agctgatgcg ccagatctgg
     8041 gtaattttga agcaaatacc accttccaga ttacttacct gtaaggcata cccccatagc
     8101 gacactgcta tgggggattt aaatggattt tgaccgaaca agaataaaaa cggatagaaa
     8161 cgtgtatgcc tgaccattct ctttttcgat tacggattct tccgtggtgc attgtgctgg
     8221 caatgtcagg gagttatagc agtgtctggg ctgaagacga cattcagttt gattcccgtt
     8281 ttctggaatt aaaaggcgac acaaaaattg atctgaagcg tttttccagc cagggatatg
     8341 ttgagcccgg aaaatacaat ttacaggttc aactaaataa acagccattg gcggaagagt
     8401 acgatattta ctggtatgct ggtgaagatg acgcgagcaa aagctatgct tgtctgacac
     8461 cggaactggt agcgcagttt ggtttaaaag aagacgtggc gaaaaatctg caatggagcc
     8521 acgatgctaa atgcctgaaa tccggtcaac tggaaggcat ggaaattaag gctgatttaa
     8581 gccagtccgc attagtcatt tcactgccac aggcttacct cgaatatact tatcccgact
     8641 gggatccgcc ttcacgttgg gatgacggca tctccgggat cgtcgcggac tacagcatca
     8701 acgcacaaac ccggcacgaa gaaaatggcg gtgatgatag taacgagatc agcggcaacg
     8761 ggacggtcgg ggttaacctg gggccgtggc gtatgcgtgc tgactggcag actaactatc
     8821 aacatactcg cagtaatgat gacgatgaat tcagcggcga tgaaactcaa aaaaaatggg
     8881 agtggagtcg ctactatgcc aggcgggcgt taccatcatt aaaagccaaa ctggcgctgg
     8941 gcgaggatta cctcagatcc gatatttttg atggttttaa ctatgttggt ggcagtgtca
     9001 gtactgacga tcaaatgttg cctcccaatc tgcgcggcta cgcgccagac atttccggcg
     9061 tggcacacac cacagcaaaa gtgaccgtca gccagatggg gcgtgtgatt tacgaaacgc
     9121 aggttccggc tggaccgttt cgtattcagg atcttggtga ttctgtctcc ggtacgttgc
     9181 atattcgcat tgaagaacag aacggccagg tgcaggaata tgacatcagc accgcctcga
     9241 tgccatacct cactcgtcca ggtcaggttc gctataagat catgatgggc cgtccgcaag
     9301 agtggggaca ccatgtcgag ggtgaatttt tttctggtgc tgaagcttcc tgggggatcg
     9361 ctaacggctg gtcgttatat ggcggcgcac tgggagatga aaactatcag tctgcggcgc
     9421 ttggcgtcgg tcgcgatttg tctacattcg gcgcggtcgc gtttgatgtt actcactcgc
     9481 acaccaaact ggataaagac accgcttatg gcaaaggttc gctggacggt aactccttcc
     9541 gtgtgagtta ttccaaagac tttgaccagc tcaacagccg cgttactttt gctggatatc
     9601 gcttctcgga agagaacttt atgaccatga gcgagtatct ggatgccagt gacagcggaa
     9661 tggtacgcac gggcaacgac aaagagatgt acaccgccac ttataaccag aacttccgcg
     9721 atgcgggtgt ttcggtttat ctcaactata cccgccatac ctactgggat cgcgaggagc
     9781 agacaaacta caacatcatg ctctcgcact atttcaatat gggcagtatt cgtaatgtca
     9841 gcatctcgat gactggctac cgttacgagt atgacaacca ggccgacaaa ggcatgtaca
     9901 tttcgctcag tatgccgtgg ggcgacaaca gtaccgttag ctataacggt actatggcag
     9961 tgggacggac agcagtcagg tcggttattt cagccgtgtc gatgacgcga ctcactatca
    10021 gttgaacgtc ggcaccagtg acaaacacac cagcgttgac ggctattaca gccatgatgg
    10081 ttcgctggcg caggttgacc tcagtgcgaa ctaccatgaa gggcaataca cctctgcggg
    10141 cttgtcgtta cagggcggcg caacgcttac tgcccacggt ggcgcactcc accgtaccca
    10201 gaatatgggc gggacacgct tgttgattga tgccgatggc gttgccgatg ttccggtaga
    10261 aggtaacggt gctgctgttt ataccaatat gtttggtaaa gccgtcgttt ctgacgtcaa
    10321 taactattac cgcaatcagg cgtatatcga cctcaacaaa ctgccggaaa acgccgaagc
    10381 aacccagtcg gtggtacaag ccacgctaac tgaaggggcg attggctacc gcaaatttgc
    10441 cgtcatcagt ggtcaaaaag cgatggcggt gctgcgttta caagacggca gccatcctcc
    10501 gtttggcgca gaagtaaaaa atgataacga gcagacagtg ggccttgtcg atgatgacgg
    10561 caatgtttat ctggctgggg tgaaacctgg cgaacacatg agtgtgttct ggagtggtgt
    10621 tgcgcattgc gatatcaacc tgccggaccc gctgcctgcc gatctgttta acggcttgtt
    10681 actgccatgc cagcataaag gcaacgtcgc gccggttatt cctgatgaca taaagcctgt
    10741 cattcaggag cagacgcaac aggtgacacc caccaatccc cctgtttctg tttcagctaa
    10801 ccaataacgt gattaaggaa tgatccatgt ctgatttact ttgctctgcc aaattgggtg
    10861 ctacgacgct agcgttactg cttagcgctg ccagtttgtc tgcccaggct tctgtaacgc
    10921 cagatcgtac ccgtttgata tttaatgaaa gcgacaaatc aatcagcgtg acgttgcgca
    10981 ataacgatcc gaaactgcct tatctggccc aaagctggat cgaagatgaa aagggtaaca
    11041 agatcagttc accgttaact gttctgccac cggtgcaacg catcgattca atgatgaacg
    11101 ggcaggtaaa agtgcaggga atgccggata tcaacaaatt gcctgccgat cgtgaaagcc
    11161 tgttctactt caacgtgcgt gagatcccgc caaaatcaaa caaagccaac acgctgcaaa
    11221 tagcactaca aacgcgtatt aagttgttct ggcgaccgaa ggcgctggag aacgtcagca
    11281 tgaaaaaccc ctggcaatat aaggttactt taacccgcaa cggtcaggaa tttaccgtca
    11341 ataacccgac gtcgtattac gtcattttca gtaatgccag cacacagaaa aatggcaatc
    11401 cggcagccgg tttctcgcca atagtgatgt cgccgaaaac ctctgcaccg ctgaatgtga
    11461 agatgggtag cgtgccggta ctgacctatg tcaacgacta tggcgctcgg atgccgctct
    11521 ttttcacttg caacggtaac a
//
LOCUS       DADLSA010000122        11491 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.122, whole genome shotgun sequence.
ACCESSION   DADLSA010000122 DADLSA010000000
VERSION     DADLSA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.122"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..711)
                     /locus_tag="JN691_003146"
     CDS             complement(<1..711)
                     /locus_tag="JN691_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HAY7244519.1"
                     /translation="MSGIFVGLNSHIMEGFDSVLTSVAADTGGWMAHLFSIAVLSLYI
                     TWRAYQTMAGKLVRPFEEIVWDVSRMLIIMIFVTNAGGYLDLVSDAITGLRDGFSGDA
                     SIWARLDTVWEKAQALGEKLYSYDDSTYVALKGTLAQFLVWAGVMLLVGITAIINLIA
                     ELVMLLMLATAPIFIYCLMWSWFRDMFSNWLRIILGCILTSLFSGIALTVVMDYLDYI
                     LAGATKASDANYLTLAVQV"
     gene            complement(729..1013)
                     /locus_tag="JN691_003147"
     CDS             complement(729..1013)
                     /locus_tag="JN691_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HAY7244520.1"
                     /translation="MKLKTLTFIVLASVAALTGCEDKTSVDWYMAHHDDMLAKYRECI
                     FNHNFVPTDCQNARSALHREKAKPDVAEGYKQILKDLDKARVQEPIADLN"
     gene            complement(1025..1735)
                     /locus_tag="JN691_003148"
     CDS             complement(1025..1735)
                     /locus_tag="JN691_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015696806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB5"
                     /protein_id="HAY7244521.1"
                     /translation="MKTTLTALTVALCLFHVPAQAGIPVFDEVANASNIQQWAEKLQQ
                     WQQTVQHYQSQMNAYKQQLATATGIRDVQAFLNQAKNLTADIKNLKNRGISLNDLLTG
                     SGGSYAGELNRLYDKYKLFDNCPENASEAYRDSCKQMLLNQAVSVEDTSDIQEKISDT
                     LSDISNLASRIENAKDSKESQDLANVVSARSIQLNALTTQWEMSVKQSEMRNQLLAEQ
                     RRKAQAEQMRTAPIADLN"
     gene            complement(1760..4510)
                     /locus_tag="JN691_003149"
     CDS             complement(1760..4510)
                     /locus_tag="JN691_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015696807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY7244522.1"
                     /translation="MTILNKALTRPAALWGVPLVPLLFTLCILTLAAVWSRFYWLLLL
                     LIPAFIELRRLAKEDIHFFDLKLLAFKTRGNRAATRHFGTKALVANQYDAIDVTEFIK
                     HMRLNESIPLSRYLPWSSHLHEQVVKNKKGDFVSTWEIGGIIFECEDEHYLATLDMQL
                     NNVIRSFEGLPVTFYTHLVSDEFTDTLECESGIPFSDEIARLFYASREGKPFYQRRLF
                     FTVCFNPHTGIERATMKQKSLGERKTLLDNALQVMQEHRQAIGTALSRYAARPLGTYE
                     ENGHVFSSQLAFYNFLITGRWQKVRVTRTPFYDVLGGTDIFPSTDIGQVSHTDGQSYF
                     RGLEIKDYSTETITGLYDSLLYARCRYVFTQSYTCMGKDEARGCIRTIEKRLKSAEDD
                     AISQQEDLKVARDMLQSGIISFGKYHQTLIVSAPTSEQVIRDTNTLADTLKDLGFIMT
                     LSRESLSAAYLAQLPGVYHLRPRLVPVSSLNYAEMVSFHNFHPHKRDGNAWGEAIAVV
                     PTPSGGCQYVNLHNSQADVDEFSEKPPGNTMLLGSTGTGKSTLLMMFQHLMQKYRRPE
                     SFAPGATKKRFTSVVFDKDRSAEMSIRLQGGSYFRFRSGEKTGLNPFALAPTKRNRNF
                     LKKLMRMMCTRDGKPLDPRDEERLSEAVDTIMLDYPPELRRNGITRMLEVLHEAPTKD
                     ARMNGLRIRLQQWAQGGEFGWVFDNDVDTFDIDNIDNFGIDGTEFLNDKDVRSPLTFY
                     LLYRVTSLLDGRRLVIFMDEFWKWLQDPEFSKFALDMLKVIRKLNGIFFPATQSPEEM
                     CKHEISAAIIEQCSTRIFMANQQAKEYDYVDMLRVPKAVYDIVTTLDPYARQMVIMKS
                     PLRRGDVRPYIAQITLDLSGLGRYTKLLSASADNLKIFDEIWQEGMKPEDWKDEMLRR
                     AV"
     gene            complement(4520..4819)
                     /locus_tag="JN691_003150"
     CDS             complement(4520..4819)
                     /locus_tag="JN691_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HAY7244523.1"
                     /translation="MTLRTIKTAATRLIAAFSALSAVPAMAASADQAFQKGSDAINWV
                     FLGLTGMSVVTISCAVAFIAYKMLVDGKAWHDLQRVFWAGVLLAGASGFGAYFAA"
     gene            complement(4830..5492)
                     /locus_tag="JN691_003151"
     CDS             complement(4830..5492)
                     /locus_tag="JN691_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAY7244524.1"
                     /translation="MLTTAALFQLAMQCAPAVHPDTIHDITRTESGLNPYAIAEIVPV
                     KGGRSRVISHLPSSKDEALKIVEAIKQKKHRYSVGLMQITSTNFPQFGVSAESMFNPC
                     DNMTVAAKIITDCYQRGGTLQRALSCYYSGNFETGQRPESAFGNTSYVQRIGYVVPST
                     RAEQQTASPSSGEAPAVPSDNTVYPDSVIRGVIPAPDTTPTSIAAYPPHVVRGGLAVS
                     SD"
     gene            complement(5495..5710)
                     /locus_tag="JN691_003152"
     CDS             complement(5495..5710)
                     /locus_tag="JN691_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244525.1"
                     /translation="MPDITPAPASLLEAVFTPMTEYTHNVYSVKGDRLAMTLTKIGVA
                     QANSQGDIQLFINPDCATGKVLLYRKD"
     gene            complement(5703..6011)
                     /locus_tag="JN691_003153"
     CDS             complement(5703..6011)
                     /locus_tag="JN691_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244526.1"
                     /translation="MSMQTVRRKALRQNPYSTLGKPPLRSSRDRITRAINGTWSLREL
                     PDNVRELLIERAALRAEITTLTVKAQGLKKSDVSVLIADRLRRIDAITSLLENRKCNA
                     "
     gene            6325..7239
                     /locus_tag="JN691_003154"
     CDS             6325..7239
                     /locus_tag="JN691_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAY7244527.1"
                     /translation="MLEMFEKIEKKYWQDKIKKISKFRVIKMQDLDDAKVIEVLAKVL
                     TKKGISKVKHNSYISKVLQITTSAGYKKLNGATKWEVQQLIKVVQSVGMDMIEFFRIY
                     YEDNKEIQDAIWNDGRSEHSCKVCLYPEGSEVNTEYSALKVADQWNIFPTNDLKDELL
                     YESKRSIESIVINPKVISSTKYRIALLDDDANITESLKEILSNDYYQVDTYDNLKKLT
                     QAVATNPYDAYVLDWIIGDETSFKLVKAIRSSKNKNAMIIVLTGQLSGIKDDEIVKSI
                     HDYDIVGPYEKPIKSGIIKSNIEKYFAR"
     gene            7243..8226
                     /locus_tag="JN691_003155"
     CDS             7243..8226
                     /locus_tag="JN691_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HAY7244528.1"
                     /translation="MRNLLEYSGSLISNDSLIVAPYDCQKLSYENILPLNNGLIKNWN
                     IQKFSSDYSEIEHLNVINGMGVTLGDSIVGISVLSAIKKKNPKIKIKLIRPETAPSYV
                     EELYELASSVIDELHYMPFHIQKLPCTALNIDVGNQLYSPDFHIMEMHDYFIQGLGVK
                     LDDVNHNELSNKWLGNIDLGAPVFENYTLFAPIASTKIRSIPSKFYYDIVDMLSRREG
                     KTVLGFVDVNHKNYINISKYSPHTKDFIALIKYASNVYTCDSSALHIAAGFGVPTECV
                     FNTIPPELRTKYYSKCKSIYVGNKRLEKIQSSEDFQLVKMVENNYREYLYG"
     gene            8219..8707
                     /locus_tag="JN691_003156"
     CDS             8219..8707
                     /locus_tag="JN691_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7244529.1"
                     /translation="MVKIISFLSLMLLSISAYSNIGDIVIDGSIANANSDKGYVITEK
                     QFDSFPRDTIVTTTPWTPTGKKVKFKGVDLSYVLKLAGANGAKLKFHALNDYEIIVDM
                     NDVEKYNILLATEMDGKRLQIRDFGPYFLVYPLDEHYIELNTPHYLARFIWQIDKITV
                     LK"
     gene            8704..10422
                     /locus_tag="JN691_003157"
     CDS             8704..10422
                     /locus_tag="JN691_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HAY7244530.1"
                     /translation="MKKNFFIILVALLLLIAFTVSSVVKYTSLLSEKNLYTVAGTQEN
                     YGFSVAKFIIQLNELYTLINSDNTIDDVRLKFDILYSRLNVIYVKSEATAPLYKQQGY
                     EETIDSINKKLEDIDGLLSHNHPDYKKISTIIADIKPLTKTVTNLADMAEIAQRNDAL
                     KDFRSKRQQLWTLLFITGGLISLLLFVLFIYISKINRLLLSERAAFASKNAFLGMVGH
                     ELRTSLQAIVSIIDVVTNNLSGGIKSGQIERLETAVSKMERQLNDLAEFAKIDNGSVE
                     IKNTYNSLQAIVTNAVQDCIAIYEKKDVTVKIKNNNDAVIFTDALRLNQVIENLTSNA
                     IKYTERGEVNVDYFIEKGKVLNIVISDTGKGIPKDKLKFIFKPFTRVVDSKSTVPGFG
                     MGLAIVAGIIRLLKGTIHISSEVNVGTTVTVRIPIKLGDSSLATAEVSASAPGDVFGV
                     LSLLVIDDNEMACSSLSSLLTNAGYIVEATTSPERALEKLLRKPYDVVLSDLQMPGMT
                     GDELYATVSSSQNPNRSTPFIFISAYSDAHSIGQAPLLTKPVRLKDINLKIHEVMSNQ
                     GRVKSE"
     gene            10686..10895
                     /locus_tag="JN691_003158"
     CDS             10686..10895
                     /locus_tag="JN691_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244531.1"
                     /translation="MLLTLRGMFFSYFSDTRELNTVVAKLYPLKMSQMPLYAVGVVPA
                     RRQPQPGRISALAGRRAAYERVPDN"
     gene            11104..11238
                     /locus_tag="JN691_003159"
     CDS             11104..11238
                     /locus_tag="JN691_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244532.1"
                     /translation="MGALSDVAEPARPSGRCKSGHSPEPVHRVGDEEVMCLQDLLKDL
                     "
     gene            complement(11281..11491)
                     /locus_tag="JN691_003160"
                     /pseudo
     CDS             complement(11281..11491)
                     /locus_tag="JN691_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2995 a         2639 c         2931 g         2926 t
ORIGIN      
        1 gacctgaacg gccagagtca ggtagttggc atcggatgct tttgtggcgc ccgccaggat
       61 gtagtccagg taatccatta ccaccgtcag ggcgatgccg ctgaacaggg atgtaaggat
      121 gcagcccaga ataattctga gccagttgct gaacatatcc cggaaccacg accacatcag
      181 gcagtagata aagatcgggg cggtcgccag catcagcagc atcaccagtt cggctatcag
      241 gttaatgatg gccgtgatgc ctaccagtaa catgacgcct gcccagacca gaaactgcgc
      301 cagagtgcct tttagagcga cataggttga atcatcataa gagtaaagct tctccccaag
      361 tgcttgagcc ttttcccaga cggtatccag ccgcgcccag atcgacgcgt cgccgctgaa
      421 tccgtcccgc aggccggtga tggcatccga aaccagatcg agatagcctc cggcgttggt
      481 gacgaaaatc atgatgatga gcatccggct cacgtcccac acgatctcct cgaaggggcg
      541 gactagcttg cctgccatcg tctggtaggc gcgccacgtg atgtacagcg agagcaccgc
      601 gatggagaac aggtgtgcca tccacccccc cgtatcggcg gccacgctgg tgagcacgct
      661 gtcaaacccc tccatgatgt ggctgtttaa tccgacgaat attccactca tggtgcgttc
      721 tcctgtcgct aattcaggtc agcaataggc tcttgcacgc gagctttatc aaggtctttc
      781 aggatttgct tatatccttc ggcaacatca ggtttagctt tttcacggtg aagggcggag
      841 cgtgcattct ggcaatcggt gggcacaaaa ttatgattaa aaatgcactc cctgtatttc
      901 gccagcatgt cgtcatggtg tgccatatac cagtctacgg aggttttatc ctcgcagcca
      961 gtaagcgcgg ccactgaggc cagaacaata aaggtcagtg tcttcagttt catggggtca
     1021 cctgttagtt aaggtctgcg attggagctg tgcgcatttg ctcggcctgc gcctttcggc
     1081 gttgttcggc aagcagctgg ttgcgcatct cactttgttt gaccgacatt tcccattgag
     1141 tggtcagggc gttcagctgg atactccgcg cggacactac gttcgccaga tcctgcgatt
     1201 ctttactgtc cttcgcattc tcgatgcgac tggcgagatt actaatatcg ctcagcgtgt
     1261 cggaaatttt ttcctggatg tcagaagtgt cctcgacgga caccgcctga ttgaggagca
     1321 tctgtttgca gctatcccgg tacgcctcgc tggcgttctc cgggcagttg tcgaacagtt
     1381 tgtatttgtc atacagcctg tttagttccc cagcataaga cccgcctgag ccggttaaca
     1441 gatcgtttaa ggaaatcccc ctgtttttca gatttttaat gtcagccgtc aggttttttg
     1501 cctgattcag gaatgcctgg acgtcccgaa taccggtggc cgttgccagc tgttgtttat
     1561 aagcgttcat ctgcgactga taatgttgaa ccgtctgctg ccactgctgg agcttctccg
     1621 cccactgctg gatgttgctg gcgttggcta cttcatcaaa aacgggaata ccggcctgag
     1681 ccgggacgtg aaagagacac aaggcgacgg tcagcgccgt cagggtggtt ttcagtttca
     1741 tggtgttatc cttttgtgat taaacggcac gtctcagcat ttcgtctttc cagtcttccg
     1801 gcttcatacc ctcctgccag atctcgtcaa aaattttcag gttgtccgca ctggcgctga
     1861 gcagcttcgt gtagcgacct agcccggaga ggtcgagggt gatttgtgcg atatacgggc
     1921 gcacgtcacc acgccgcagc ggagatttca tgatcaccat ctggcgggcg taggggtcga
     1981 gggtggtcac gatgtcgtaa accgctttcg gcacccgcag catgtccacg tagtcgtact
     2041 ccttcgcctg ctggttcgcc atgaagatgc gggtactaca ctgctcgata atggccgccg
     2101 agatctcgtg cttgcacatc tcctccggcg actgcgtggc cgggaagaaa atgccgttga
     2161 gctttcgaat gaccttcagc atgtcgaggg cgaattttga aaactccggg tcctgaagcc
     2221 atttccagaa ttcatccatg aaaatgacca ggcggcggcc atcgaggagg ctggtcacgc
     2281 ggtagagcag gtagaaggtg agcgggctgc gcacgtcttt gtcgttcagg aactccgtgc
     2341 cgtcgatgcc gaaattatca atattgtcga tatcaaaggt gtccacgtca ttatcaaaca
     2401 cccagccaaa ctccccgccc tgcgcccact gctggagacg gatgcgcagc ccgttcatcc
     2461 gtgcgtcctt cgtcggggct tcatgcagca cttccagcat gcgggtgatg ccgttgcgac
     2521 gaagctcagg cgggtagtcc agcatgatgg tgtccactgc ctcgctgagg cgttcttcat
     2581 cgcgcgggtc gagcggtttg ccgtcccttg tgcacatcat tcgcatcagc ttcttgagga
     2641 agttgcggtt acgtttcgtt ggagcgagcg caaacgggtt aaggccggtc ttctcgcctg
     2701 aacggaaacg gaagtacgag ccgccctgga ggcgtatcga catttccgcc gaacggtctt
     2761 tatcaaagac caccgaggtg aagcgctttt tcgtcgcccc cggcgcaaac gactcaggac
     2821 gccggtattt ctgcatcaga tgctggaaca tcatcagcag ggtcgatttc cccgtcccgg
     2881 tactgccgag cagcatggtg ttacccggcg gcttctcgct gaactcgtca acatcggcct
     2941 ggctgttgtg cagattcacg tactgacatc ccccggacgg ggtcggaaca acggcaatcg
     3001 cctcccccca ggcattcccg tcccgcttgt gcgggtgaaa gttgtgaaac gacaccattt
     3061 cggcgtagtt gaggctgctc accggcacca gacgcgggcg caggtgatac acacccggca
     3121 actgcgcgag atacgccgcc gacagcgact cgcgggacag ggtcatgatg aaaccgagat
     3181 ccttcagggt atccgccagg gtgttggtgt cccggataac ctgctcgctg gttggggcgc
     3241 tgacaataag cgtctggtga tatttcccga atgaaataat gccggactga agcatgtcgc
     3301 gggcgacctt caggtcttcc tgctgggaga tcgcatcatc ctcggcagac ttcaggcgtt
     3361 tctcgatggt gcggatgcag ccccgcgctt catccttacc catgcaggta taggactgcg
     3421 tgaacacgta ccggcagcgg gcataaagaa gcgagtcata cagcccggtg atggtttcag
     3481 tgctgtaatc cttgatttcc agcccgcgaa agtagctctg gccgtcggtg tggctgacct
     3541 ggccaatgtc ggtggaaggg aaaatatccg tgccgcccag cacgtcataa aacggcgtgc
     3601 gggtgacgcg gactttctgc cacctgccgg tgatcagaaa gttgtaaaac gccagctggg
     3661 aggagaacac gtgcccgttc tcctcataag tgccaagcgg ccgcgccgcg tagcgtgaca
     3721 gcgccgtgcc aatcgcttgc cggtgctcct gcatcacctg cagggcattg tcgagcaggg
     3781 ttttacgttc gcccagggac ttctgtttca tggtcgcccg ctcaatgccg gtgtgcggat
     3841 tgaagcagac ggtgaagaag aggcgacgct ggtagaaagg tttgccctcg cgggaggcgt
     3901 aaaacagacg cgcgatttca tctgaaaacg gaatcccgga ttcacattca agcgtgtcgg
     3961 taaattcgtc gctcaccaga tgggtataga aggtaacggg tagcccctca aaggagcgga
     4021 taacgttgtt gagctgcatg tccagggtgg cgaggtaatg ttcatcctcg cattcaaaaa
     4081 tgataccgcc gatctcccag gtgctgacaa agtcaccttt tttgttcttc accacctgct
     4141 cgtgcaggtg ggaagaccag gggagatagc gggacagcgg gatactctcg ttgagccgca
     4201 tatgttttat aaactccgta acgtctatgg cgtcgtactg gtttgcgaca agcgcctttg
     4261 tcccgaaatg ccgggtcgcc gcgcggttgc cacgcgtttt aaacgccagc aacttcaggt
     4321 caaagaaatg aatgtcctct tttgccagcc gtctcagctc gataaaggca ggtatgagaa
     4381 gcaggagaag ccagtaaaaa cgtgaccaga cggccgccag ggtaaggatg cacagggtga
     4441 acagcagggg gacaagcggg accccccaca gcgccgccgg tcgggtcagc gccttgttga
     4501 ggatggtcat gctgcctcct taggcggcga agtaggcacc gaacccggac gccccggcga
     4561 gcaggacacc cgcccagaac acgcgctgga gatcgtgcca ggccttgccg tcaacaagca
     4621 tcttgtaggc gataaaggcc accgcacagc tgatagtgac gactgacata ccagttaggc
     4681 cgaggaagac ccagttaatg gcgtcactgc ctttctgaaa tgcctggtca gcgctggccg
     4741 ccattgctgg aacagcagac agggcggaaa atgcggcaat caggcgggtt gcagcggttt
     4801 tgatagtacg taaagtcatg atctcttcct taatcagaag acacagcgag gccgccgcgc
     4861 acgacatgtg gcggataagc agcgatgctg gtcggggtgg tgtcgggtgc cggaattacg
     4921 ccgcgtatga cgctatccgg gtaaacagtg ttgtcggacg ggacggcagg cgcttccccg
     4981 gatgagggag atgctgtctg ctgttctgcc cgcgttgagg gcacaacgta gccaatacgc
     5041 tgtacgtagc ttgtgttgcc gaacgcgctt tccgggcgct ggccggtttc aaagttgcct
     5101 gagtaatagc agctcagggc gcgctgtaat gttcctcctc gttgatagca gtcggtgatg
     5161 attttcgcgg caacagtcat gttgtcacag gggttaaaca tggattcggc gcttacgccg
     5221 aactgcggga agttggtgct ggttatctgc atcagcccta ctgaataacg gtgctttttc
     5281 tgctttatgg cctccacaat ttttagcgcc tcgtctttcg acgaaggcag atgagaaatc
     5341 acgcggctcc tgccgccctt taccgggaca atctcagcga tggcatatgg atttaagccg
     5401 gattcggtgc gggtgatgtc gtggatggta tcggggtgaa cggcgggagc acactgcatt
     5461 gcgagctgaa acaacgcggc agtggttaac atagtcagtc ctttctgtag agcagtacct
     5521 taccggtggc acagtccggg ttgataaata actgaatgtc gccctggctg ttagcttggg
     5581 caacaccgat ttttgtaagc gtcattgcca gccgatcacc cttcaccgaa tacacgttgt
     5641 gcgtgtactc ggtcatgggg gtgaatacgg cttcaagcag tgaggccgga gcaggcgtga
     5701 tgtcaggcat tacatttcct attttccagc agtgaggtga ttgcatcgat acgtcgcagc
     5761 ctgtcggcaa taagtacgga tacatcggat tttttgagtc cctgcgcttt cacagtcagg
     5821 gtagtgattt ctgcccgcag ggctgcccgt tcaattagca attctcggac gttatccggc
     5881 agctccctga gcgaccaggt gccattaatg gcacgcgtga tacggtcacg ggaggagcgt
     5941 agcggcggct tgccgagagt tgaataagga ttctgacgca gcgccttacg gcggacggtt
     6001 tgcatgctca tggctgtttc tcctgggtat gagtaagcca cccgcgtgcg gtgcacagtg
     6061 ggagacaggg aaggattaaa cgggaggctt aacgatacag aggagtgaga gtgttaccca
     6121 ttacaggcgc ggcacgtcat ctctttacgc agtcacagaa aacacctcct tatcctgacc
     6181 ggggaacgcc gctgctaggt tattaaagag ctaatgattt gattaataca atagtgctta
     6241 taagttgggt attcaataaa attataactt atagtgtcga tttaagaatg attccaattt
     6301 ttaggacttt ttcagctgtg taaaatgttg gagatgtttg aaaaaattga aaaaaaatac
     6361 tggcaggata aaatcaagaa aatctccaaa ttcagagtaa taaaaatgca agatctcgat
     6421 gatgcaaaag tgatagaggt actggcgaag gtattgacca aaaaaggtat ttccaaggta
     6481 aagcacaata gttacataag taaggtgcta cagatcacta catcagcggg ctataagaag
     6541 ttgaatggtg cgaccaagtg ggaggttcag caactcataa aagtagtcca atccgttggc
     6601 atggatatga ttgagttttt ccgtatttat tatgaggata ataaagaaat tcaagatgcg
     6661 atctggaacg atggccgcag tgaacactct tgtaaggtat gcctctatcc tgaaggttcg
     6721 gaagtaaaca cagaatactc agcactgaaa gtagctgacc aatggaacat ttttccgaca
     6781 aatgatttaa aagatgaact gctttatgaa tcaaaacgaa gcatagaaag tattgtcata
     6841 aatccaaagg taattagcag cactaagtat cgaattgcac tacttgatga cgatgctaat
     6901 atcaccgaaa gcttaaaaga aatcctttcg aacgattatt atcaagtaga tacttatgat
     6961 aacttgaaaa aacttactca agctgtcgca acaaatccgt atgacgcata tgttttggac
     7021 tggatcatcg gtgatgaaac aagctttaag ttggttaaag ctataaggtc gtcaaaaaat
     7081 aaaaatgcaa tgataatcgt tcttacaggt caactatctg gtataaaaga tgatgaaatt
     7141 gtaaagtcaa ttcatgatta tgacattgtg ggaccatacg aaaagccgat taaatcaggc
     7201 atcatcaaat ctaacattga aaaatatttc gccagataga ttatgagaaa tttacttgaa
     7261 tattccggta gcttgataag taacgatagt ttgatcgtag caccttacga ttgtcagaag
     7321 ctaagctatg aaaatatttt accattaaac aatggtctta taaaaaactg gaatatacaa
     7381 aaattctcaa gtgattactc tgaaatagaa cacctaaatg tgatcaatgg catgggagtg
     7441 acattaggtg attctatcgt cgggatttct gtgctgtcag caatcaaaaa aaagaatccc
     7501 aagattaaaa ttaagcttat tcgaccggag actgcaccgt catatgttga ggaactgtat
     7561 gaattagcat cctcggttat agatgagctt cattacatgc cttttcatat tcagaaattg
     7621 ccttgtactg cattgaatat tgatgtggga aatcagctat attctccaga tttccatatt
     7681 atggaaatgc atgattattt tatccaaggt cttggcgtga aacttgatga tgttaaccat
     7741 aatgagctat ctaataaatg gttaggcaat attgatctcg gagcgccagt ttttgaaaac
     7801 tacacacttt ttgcacctat tgctagcaca aaaataagaa gtataccgtc gaagttctat
     7861 tatgacatag ttgatatgct ttctaggcgc gaaggaaaaa cggtactagg tttcgtggat
     7921 gtgaatcata aaaattatat taacatatca aagtattcac cccatacgaa agatttcata
     7981 gcgctaatta agtatgctag caacgtttat acttgtgact cctctgcatt gcatatagcc
     8041 gctggatttg gtgtacctac tgaatgcgtg tttaatacaa taccgccaga attaagaaca
     8101 aagtattaca gtaagtgtaa gtcgatttat gttgggaata aaagattaga aaaaattcag
     8161 agtagtgaag atttccaact ggttaaaatg gtggaaaata attacaggga gtatctttat
     8221 ggttaaaata atatcattct tatccctaat gcttttgtca attagtgcat atagcaatat
     8281 tggggatata gttatagatg gttcaatcgc taatgctaac agtgataaag gttatgtgat
     8341 tactgaaaag cagtttgatt cgttcccacg ggataccatc gtaacgacaa caccttggac
     8401 gccaaccggt aaaaaagtaa aattcaaggg cgtcgaccta agttatgttt taaaactcgc
     8461 tggcgctaat ggagcaaagc taaaattcca tgctctgaat gattatgaaa tcattgtaga
     8521 catgaatgac gttgaaaaat ataacatttt attggctaca gaaatggatg gaaaaaggct
     8581 tcaaataagg gattttggac cttatttttt ggtatatcct ttagatgaac attatattga
     8641 gcttaacacg ccacactatc ttgcacggtt catttggcag atagacaaaa ttacggtgct
     8701 caaatgaaaa aaaatttctt tatcattttg gttgctctgc ttttgttgat agcattcact
     8761 gtgtctagtg ttgttaagta caccagtctt ttatcggaga aaaatttata caccgtcgct
     8821 ggcacccaag aaaactacgg tttctcagtg gcaaaattca ttatacagtt gaatgagctt
     8881 tacacgctaa tcaatagcga taatactatt gacgatgtta ggcttaaatt cgatatcctt
     8941 tattctcgtt tgaatgttat ttatgtaaag agtgaggcga ctgcacctct atataaacag
     9001 caaggctatg aagaaacaat tgattcgata aacaaaaagc ttgaagacat agacgggctg
     9061 ctttcgcata atcaccctga ttataaaaaa ataagtacaa tcatagctga tataaaaccg
     9121 ctaactaaaa ccgttacaaa tcttgctgat atggctgaaa tagcacaaag gaatgacgct
     9181 cttaaagatt tcaggagcaa acgtcagcag ttatggactc tgctttttat tacaggtggg
     9241 ttgatttcct tacttctttt tgtcctattt atttacataa gtaaaattaa ccgattgtta
     9301 ctatcagaac gggctgcttt tgccagtaaa aatgcatttt taggtatggt aggccatgaa
     9361 ctcagaacat ctttacaggc aatagtgtct atcattgatg tcgtaaccaa taatctgagt
     9421 ggagggatta aatctggtca gatagaacga cttgaaactg ctgtttcgaa aatggagcgc
     9481 cagttaaatg atttggctga atttgcaaaa attgacaacg gatctgtaga gataaaaaac
     9541 acctataata gcttgcaagc aattgttact aacgctgtgc aggattgcat tgctatttat
     9601 gaaaaaaagg atgtcacagt taaaataaaa aacaataatg atgcagtgat ttttacagat
     9661 gctctacgcc ttaatcaagt aattgaaaac ttaaccagta acgctattaa gtacactgag
     9721 cgcggggaag ttaatgtaga ttactttata gaaaaaggta aggtattaaa tattgtcatt
     9781 tctgataccg gaaagggaat ccctaaagac aaactaaagt tcatatttaa accatttaca
     9841 cgagttgtag attcgaaatc cactgtacct ggatttggga tgggacttgc tattgttgca
     9901 gggataatca gactattaaa aggtactatt catatatcta gtgaagtaaa tgtaggtaca
     9961 actgttacag tgagaatacc tatcaagttg ggagatagca gccttgcaac agctgaggta
    10021 tctgcttcag ctcccggaga tgtcttcggc gttcttagct tgcttgttat tgatgataat
    10081 gaaatggcct gttcgtctct ttccagtctg cttactaatg ctggatacat tgtcgaggct
    10141 acaacctcgc ctgaacgagc cctggaaaag cttctgcgta aaccttatga cgttgtcctg
    10201 agtgatttac aaatgccagg catgacaggt gatgagctgt atgcaactgt tagtagcagt
    10261 cagaatccta atcgatccac gcctttcata ttcatcagcg catacagtga tgcacactct
    10321 atcggacagg ctcctttgtt aactaaacct gtcaggttaa aagatataaa ccttaaaata
    10381 catgaggtta tgtcaaatca aggacgagtc aaaagtgagt aggcattgtc gtaatatttg
    10441 cgattatagc tttggtgttc tcgatcgttg cgaattttga aaatttgtgg agtcaaatgg
    10501 cgccgaaagc ccggcgtagc cggttacctg gctataaact ctgccaccct accccctgcc
    10561 ctgattagcc ttaacccgct tacagcaatg aagaagtaaa agtaatccct tttaaccccg
    10621 gtgagcgggc tttacggcgt ttacgacgta aagggcgtga tttccggggc cttagcggcg
    10681 gcagcttgct gctgacgctt agggggatgt tcttcagtta tttcagcgac accagggaac
    10741 ttaacacggt agttgcaaag ctatacccgc taaaaatgtc tcagatgcct ctgtacgcgg
    10801 tcggagtggt cccggcaagg cgtcagccgc agccggggcg tatctccgcg ttagcgggcc
    10861 gaagggccgc ttacgagcgt gtacccgaca actgaccgca gctccaccca cagcgatgaa
    10921 gaagagtagt tacaacaccc atcattaaaa gcgacactac tcccggcctt cggccggggg
    10981 cggcggcgct tttcagtggt ggggaggggc acccggccgg caaaccggcg ccggttttat
    11041 gatgagcttt cttttgaatg ctgtaactaa tttggttctc cggtcggggc ccttcgcgaa
    11101 gcgatgggag ctctttctga tgttgccgaa ccggcgcgac cgtcaggtcg ctgcaagagc
    11161 gggcattctc cggagcctgt ccacagggta ggcgatgaag aggttatgtg tttacaggat
    11221 ttattaaagg atctttaaaa gagatacaga actggtgttt aaaaggaact acgcgccctg
    11281 aaagaccgac ggcaggctca gaagatgctc cagcgtggcc atagtacgtt caccgaagac
    11341 gtgtgcaaca accgtcctcc gtatcctgtc atacgcgtaa aacaaccagc gctggcgtga
    11401 tttagcgccg acgtaacccc actgttcgtc catttccgcg caaacaatga cgtcactgcc
    11461 cggttgtatg cgtgagttta ccgactgcgg c
//
LOCUS       DADLSA010000123        11142 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.123, whole genome shotgun sequence.
ACCESSION   DADLSA010000123 DADLSA010000000
VERSION     DADLSA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.123"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(327..1991)
                     /gene="glnS"
                     /locus_tag="JN691_003161"
     CDS             complement(327..1991)
                     /gene="glnS"
                     /locus_tag="JN691_003161"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAY7244533.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2194..4140)
                     /gene="nagE"
                     /locus_tag="JN691_003162"
     CDS             complement(2194..4140)
                     /gene="nagE"
                     /locus_tag="JN691_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAY7244534.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAVVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAVGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISLVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4473..5273
                     /gene="nagB"
                     /locus_tag="JN691_003163"
     CDS             4473..5273
                     /gene="nagB"
                     /locus_tag="JN691_003163"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY7244535.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5333..6481
                     /gene="nagA"
                     /locus_tag="JN691_003164"
     CDS             5333..6481
                     /gene="nagA"
                     /locus_tag="JN691_003164"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY7244536.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6490..7710
                     /gene="nagC"
                     /locus_tag="JN691_003165"
                     /gene_synonym="nagR"
     CDS             6490..7710
                     /gene="nagC"
                     /locus_tag="JN691_003165"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAY7244537.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLTSEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7758..8510
                     /gene="nagD"
                     /locus_tag="JN691_003166"
     CDS             7758..8510
                     /gene="nagD"
                     /locus_tag="JN691_003166"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HAY7244538.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEEAVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8907..10571
                     /gene="asnB"
                     /locus_tag="JN691_003167"
     CDS             8907..10571
                     /gene="asnB"
                     /locus_tag="JN691_003167"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAY7244539.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10951..11027
                     /locus_tag="JN691_003168"
     tRNA            10951..11027
                     /locus_tag="JN691_003168"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10985..10987,aa:Met,seq:cat)
     gene            11036..11120
                     /locus_tag="JN691_003169"
     tRNA            11036..11120
                     /locus_tag="JN691_003169"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11070..11072,aa:Leu,seq:tag)
BASE COUNT         2784 a         2864 c         2810 g         2684 t
ORIGIN      
        1 cagcaagatg aaagcggtta cattttattt aattatgttt tgtgatttta aatttatttt
       61 atggataaat tgcgaagcgg aattaattcg cgaagaaaag cagaaaaaac gccgcagagc
      121 gcggcgtttg ggattgtttg ctatttcttg ccggatgcgg cgtgaacgcc ttatccggcc
      181 tacaaaagca cgcaaattca atatattgca gagatcatgt aggcctgata agcgaagcgc
      241 atcaggcaat ttagcgtttg aaatttattg tctatttatt gccggatgcg acgtaaatgc
      301 ctcacccggc ttggtagaac ttaaaattac tcgcctactt tcgcccaggt atcacgcagc
      361 ccaacggtgc ggttaaatac cggtttttcg gccgtggaat ggcggctgtc gaggcagaag
      421 taaccttcac gctcaaactg gaatgcttta cccgctaccg catctttcag cgacggttca
      481 gcaaagccct gtttgatcac cagcgattcc gggttaatca ccgacaggaa atcatccgca
      541 gcacctgggt taggcacgct gaacagacga tcatacaaac ggatttcaac cggcagcgca
      601 tgtgccgcgc tcacccagtg aataacacct ttgactttac gaccatctgc cggatctttg
      661 cttaaggtgt cggcgtcata agtacagaag atggtggtga tattaccttc ggcatctttc
      721 tccacgcgtt cagccttaat gacgtaagca ttacgcagac gcacttcttt acccagcacc
      781 agacgtttgt actgcttgtt agcttcttcg cggaaatcgg cgcgatcgat ccaaatttca
      841 ccgctaaacg gcacctgacg gctccccatt tccggtttgt tcggatggtt cggcatggtg
      901 accatttcgc cttcgccctg atagttttcg ataaccagtt tcaccggatc gataaccgcc
      961 attgcgcgcg gcgcattttc gttgagatct tcacggatgc aggattccag cgacgccatc
     1021 tcaatggtgt tgtcctgctt ggtcacgccg atgcgtttgc agaactcacg aatagaagcc
     1081 gcagtgtaac cacgacgacg cagaccggaa atggtcggca tacgcgggtc atcccagcct
     1141 tcaacgtgct tgtcggtcac cagcaggttc agcttacgct tggacatcac agtgtattcc
     1201 agattcaggc gcgagaattc gtactggcgc ggatgaacag gaatggtgat gttatccagt
     1261 acccagtcgt acagacgacg gttgtcctgg aactcaagcg tacacagaga gtgcgtaata
     1321 ccttccagcg catcgctgat gcaatgggtg aagtcgtaca tcgggtagat gcaccacttg
     1381 ttgccagtct ggtggtgttc agcaaattta atgcggtaca gcaccggatc gcgcattacg
     1441 ataaatggcg acgccatgtc gattttcgca cgcaggcagg ctttaccttc ttcaaaacca
     1501 ccggcacgca ttttttcgaa cagcgccaag ttctcttcaa cgctgcggtc gcggtacggg
     1561 ctgtttttac ccggctgcgt cagggtaccg cggtattcgc ggatctgttc cggtgtcagt
     1621 tcatcaacgt acgccaggcc tttattgatc agttcgatcg cataggcgtg gagctgatca
     1681 aaataatcgg aggagtaacg gacgttacca gaccagtgaa aacctaacca ctctacgtcg
     1741 tttttgatcg aatcgacata ctcgatatct tcttttaccg ggttagtgtc gtcgaaacgc
     1801 aggttgcact ggcctttata gtcctgggcg atcccgaagt tcaggcagat agatttcgca
     1861 tggccaatat gcagatagcc attcggctcc ggcgggaaac gggtgtgtac tgtggtgtgc
     1921 ttaccactgg ccagatcttc atcgatgatc tgacggataa agttagtcgg gcgggcttct
     1981 gcctcactca tcgtggattc ctcaaagcgt aaacaacgta taacggcgta tgatcttata
     2041 agcgggacag gctgacaact gttagttttt taaaattgat aatctaacaa ggattatggg
     2101 gatgattgat gaaaaaaaaa gcggcagaga agatctccac cgcttgtcat tgttggatgc
     2161 gacgctgccg tgtcgcatca ggcataaagc agattacttt ttgatttcat acagcggtgt
     2221 ttgacccgcc acaacatggc cctgagcttt aatgatcaag ccactgaaat cgtcgatatt
     2281 gctgcaaacc accaggctaa tcatcgagcg ggcgttagcg ttcaggtaat ccagatccat
     2341 ttccagaatc ggttgccctg cgcttacctg cgcaccctct tccaccagac gtttaaagcc
     2401 tttaccttcc agcgctacgg tgtcgatacc catatggacg acgatctccg cgcctttttc
     2461 ggtttccagg cagaacgcgt ggttggtgtt gaagattttc acgattgtcc ctgcggctgg
     2521 tgatacgacg attttatctg tcggtttcac cgccacaccg tcacccaccg ctttgctggc
     2581 gaatgcttcg tcaggaacct gatccagtgc cacgacatca ccggtaatcg gcgataccag
     2641 ctccgcgata gataccgcgt ttggtacagc ctgcggtttt gctacaggcg cggcagttgc
     2701 cggagttgct tcagctgacg cagcggctac cggaccacgg gcaacgactt tcttcatcgc
     2761 atcgccgatg gattctgctt tcgcgccaac aatcacctga atagtctgtt tgttcagttt
     2821 cactacacca gaagcaccca gacgtttaca catcgtatcg ttgacgcggg cagagtcagc
     2881 cacggtcagg cgcagacggg taatacaggc atcaatcgct ttcaggttgt cagtaccgcc
     2941 aaccgcagca atatagttgg ttgccagttg agtcagacct tcttcagtat tgctgttggc
     3001 ttcttcagta acgatctcgt cttctttatc ttcacgaccc ggcgttttca ggttgaacat
     3061 gcggataacc aaactgaaca ccacgaagta gatagcgaag aagataacgc ccatcaccag
     3121 cagcatccag acgttctggc tggcggccgg caggttatac atcaacgcgt agtcgatagc
     3181 ccccgcagag aaagagaagc cggcatggat acccagcagc gttgccacaa acaggctgat
     3241 accggtcagc agtgcgtgca ggaggtacag cagcggcgca aggaacatga acaggaattc
     3301 cagcggctca gtcacaccgg tcaggaacgc agtaacagca acagaaagca gcatcccgcc
     3361 aaccatcgga cgacgctctt tcggtgctgc gaagtacatc gccagcgccg cacccggcag
     3421 accgaacatc atgatcggga agaagccgga catgaacatc cccgcggtgc cgtcaccagc
     3481 gtagaaacgg ttgatgtcgc cgtggaaaac cgtacccacc gcgttggtga attcaccaat
     3541 ctggaaccag gcgatggtgt tcagcacctg atgcagaccg gttgggatca gcagacggtt
     3601 gatgaaacca aagataccgg aacccagcgc gcccgcagaa acgatccact cgccgcctgc
     3661 atggatagcg tgctgtaccg gcggccagac gtaaccaaaa atggccgcca gcaccagaca
     3721 gaagaagccg gtggcgatcg gcacaaagcg tttgccgccg aagaagctca ggaagtccgg
     3781 cagtttaata tcggaccaac ggttataggc tgcaccacca accagaccgg taatgatacc
     3841 cgccagtaca cccatgttaa tttctgggtt gatggtcacc accgctttgg ttaacacaaa
     3901 gtaacctacc gcacccgcca gcgccgccgc acctgcgctg tctttcgacc agctggatgc
     3961 cacaccgatg gcgaagatta atgcgaggtt atcaaaaatt gcaccgcccg cctgggcaat
     4021 aaacgcaacg ttaagtaaat ctggctgacc gaatcgcagc aacagcgccg ccaccggcag
     4081 caccgcgata gggagctgta acgccctacc gagtcgctgg aaaaaaccta aaatattcat
     4141 cttattcccc ctacgagaac cctatttggc tcgtttaaag ccgtattttt attttgctgc
     4201 aaattttact gccgatgttc tgtaatcaga ttgttagatc atctgctaca gagtgtgtga
     4261 aaatttaatt cgtatcgcaa attaaacgcg tgtcttttgt gagttttgtc accaaatatc
     4321 gttatcatca ctccctttta ctggctaaac cagaaaactt attttatcat tcaaaaaatc
     4381 aagtcggatt gacgcctgtc tgcgcaaatc caggttacgc ttaaagatgc ctaatccgcc
     4441 aacggcttac attttactta ttgaggtgaa taatgagact gatccccctg actaccgctg
     4501 aacaggtcgg caaatgggct gctcgccata tcgtcaatcg tatcaatgcg ttcaaaccaa
     4561 ctgccgatcg tccgtttgta ctgggcctgc cgactggcgg cacgccgatg accacctata
     4621 aagcgttagt cgaaatgcat aaagcaggcc aggtcagctt taagcacgtt gtcaccttca
     4681 acatggacga atatgtcggt ctgccgaaag agcatccgga aagctactac agctttatgc
     4741 accgtaattt cttcgatcac gttgatattc cagcagaaaa catcaacctt ctcaacggca
     4801 atgccccgga tatcgatgcc gagtgccgcc agtatgaaga aaaaatccgt tcttacggaa
     4861 aaattcatct gtttatgggc ggtgtaggta acgacggtca tattgcattt aacgaaccgg
     4921 cgtcttctct ggcttctcgt actcgtatca aaaccctgac tcatgacact cgcgtcgcga
     4981 actctcgttt ctttgataac gatgtcaatc aggtgccaaa atatgccctg actgtcggtg
     5041 ttggtacact gctggatgcc gaagaagtga tgattctggt gctgggtagc cagaaagcac
     5101 tggcgctgca ggccgccgtt gaaggttgcg tgaaccatat gtggaccatc agctgtctgc
     5161 aactgcatcc gaaagcgatc atggtgtgcg atgaaccttc caccatggag ctgaaagtta
     5221 agactttaag atatttcaat gaattagaag cagaaaatat caaaggtctg taattgttat
     5281 ccctgccctg gttccttgct cagggcaata ttttttaaaa tcgggggtca gaatgtatgc
     5341 attaacccag ggccggatct ttaccggcca cgaatttctt gatgaccacg cggttgttat
     5401 cgctgatggc ctgattaaaa gcgtctgtcc ggtagcggaa ctgccgccag agatcgaaca
     5461 acgttctctg aacggggcca ttctctcccc cggttttatc gatgtgcagt taaacggctg
     5521 cggcggcgta cagtttaacg acaccgctga agcggtcagc gtggaaacgc tggaaatcat
     5581 gcagaaagcc aatgagaaat caggctgtac taactatctg ccgacgctta tcaccaccag
     5641 cgatgagctg atgaaacagg gcgtgcgcgt tatgcgcgag tacctggcaa aacatccgaa
     5701 tcaggcgtta ggtctgcatc tggaaggtcc gtggctgaat ctggtaaaaa aaggcaccca
     5761 taatccgaat tttgtgcgta agcctgatgc cgcgctggtc gatttcctgt gtgaaaacgc
     5821 cgacgtcatt accaaagtga ccctggcacc ggaaatggtt cctgcggaag tcatcagcaa
     5881 actggcaaat gccgggattg tggtttctgc cggtcactcc aacgcgacgt tgaaagaagc
     5941 aaaagccggt ttccgcgcgg ggattacctt tgccacccat ctgtacaacg cgatgccgta
     6001 tattaccggt cgtgaaccgg gcctggcggg cgcgatcctc gacgaagctg acatttattg
     6061 cggtattatc gctgatggcc tgcatgttga ttacgccaac attcgtaacg ctaaacgcct
     6121 taaaggcgac aaactgtgtc tggttaccga cgccaccgcg ccagcaggtg ccaacattga
     6181 acagttcatt tttgcgggta aaacaatata ctaccgtaac ggactttgtg tggatgagaa
     6241 cggtacgtta agcggttcat ccttaaccat gattgaaggc gtgcgtaatc tggtcgaaca
     6301 ttgcggtatc gcactggatg aagtgctgcg tatggcgacg ctctatccgg cgcgtgcgat
     6361 tgacgttgaa aaacgtctcg gcacgctcgc cgcaggtaaa gtagccaacc tgaccgcatt
     6421 cacacctgat tttaaaatca ccaagaccat cgttaacggt aacgaggtcg taactcaata
     6481 agagaaagta tgacaccagg cggacaagct cagataggta atgttgatct cgtaaaacag
     6541 cttaacagcg cggcggttta tcgcctgatt gaccagtacg ggccaatctc gcggattcag
     6601 attgccgagc aaagccagct tgcccccgcc agcgtaacca aaattacgcg tcagcttatc
     6661 gaacgcgggc tgatcaaaga agttgatcag caggcctcca ccgggggccg ccgcgctatc
     6721 tccatcgtca ccgaaacccg caatttccac gcaatcggcg tacggcttgg tcgtcatgac
     6781 gccaccatca ctctgtttga tctcagcagc aaagtgctgg cagaagaaca ttacccgctg
     6841 ccggaacgta cccagcagac gctggaacat gccctgctga atgccattgc tcagtttatt
     6901 gatagctacc agcgcaagct gcgcgagcta atcgccattt cggtgatcct gccagggctt
     6961 gttgacccgg acagcggcaa aattcattac atgccgcata ttcaggtaga aaactggggg
     7021 ctggtagaag cactggaaga acgttttaaa gtgacctgtt tcgttggtca cgatatccgt
     7081 agtctggcgc tggcggagca ctacttcggt gcaagtcagg attgcgaaga ctccattctg
     7141 gtgcgtgtcc atcgcggaac cggggccggg attatctcta acgggcgcat ttttattggc
     7201 cgcaacggta acgtcggtga aattggccat attcaggtcg aaccgctggg tgaacgctgc
     7261 cactgcggca actttggctg cctggaaact atcgccgcca acgctgccat tgaacaacgg
     7321 gtgttgaatc tgttaaagca gggctaccag agccgtgtgc cactggacga ctgcaccatc
     7381 aaaactatct gcaaagccgc gaacaaaggc gatagcctga cctcggaagt gattgagtat
     7441 gtcggtcgtc atctgggtaa aaccatcgcc attgctatca acttatttaa tccgcaaaaa
     7501 attgttattg ctggtgaaat caccgaagcc gataaagtgc tgctccctgc tattgaaagc
     7561 tgcattaata cccaagcgct gaaggcgttt cgtactaatc tgccggtggt acgttctgag
     7621 ctggatcacc gctcggcaat cggcgctttt gcgctggtaa aacgcgccat gctcaacggt
     7681 attttgctcc agcatttgct ggaaaattaa tgtgctttta tagtggcgct tattgttgtc
     7741 aatattctgg gtagtccatg accattaaaa atgtaatttg cgatatcgac ggcgtgctga
     7801 tgcacgataa cgtcgccgta ccgggtgcag cggaattttt gcacgggatt atggataaag
     7861 gcctgccgct ggtgttgctg accaactatc cttcgcagac tgggcaagat ctggcgaacc
     7921 gctttgccac tgccggtgtc gatgtgccgg atagcgtgtt ttatacctct gcgatggcga
     7981 ccgccgattt cctgcgtcgc caggaaggca agaaagcgta tgtggtgggc gaaggcgcac
     8041 tgattcatga actgtacaaa gccggtttca ctattaccga tgtgaaccct gatttcgtga
     8101 ttgttggcga aacacgttcc tacaactggg acatgatgca taaagcagcc tatttcgtcg
     8161 ctaacggtgc acgctttatc gccaccaacc cggacaccca cgggcgcggt ttttatcccg
     8221 cttgtggcgc gttgtgtgca gggattgaga aaatctccgg gcgcaaaccg ttctatgttg
     8281 gtaagcccag cccgtggatc atccgcgcgg cattaaacaa aatgcaggcg cattcggaag
     8341 aagcagtgat tgtcggcgat aacctgcgta ccgatattct ggcgggcttc caggccggtc
     8401 tggagacgat tctggtgctt tctggtgttt catcgctcga cgatatcgac agtatgccgt
     8461 tccgcccaag ctggatttac ccgtcagtcg ctgaaatcga cgttatctga ataccaccca
     8521 cgtctgcgga cgtggttggc tctgatgaca aacgccaaac tgcctgatgc gctacgctta
     8581 tcaggcctac gcagctcctg caatatattg aattttcatg cttttgtagg ccggataagg
     8641 cgttcacgcc gcatccggca tgaacaaatc gcactttatc agcaatttgg cccccgtaaa
     8701 atataaccat tcaataaaac ccgctaaaaa ttatcgattc atcaataaat acatcctcac
     8761 accagaccct ttttcaccaa tcgacaatca ccattacgtt ttttattttt gcactgccaa
     8821 atagcttgcg cacagtactg ctttgcggca tttttttaaa caagcaaaca caacaagcaa
     8881 caaataccag gttaacggag aaggttatgt gttcaatttt tggcgtattc gatatcaaaa
     8941 cagacgcagt tgagctgcgt aagaaagcac tcgagctgtc acgcctgatg cgtcatcgtg
     9001 gcccggactg gtccggtatt tatgccagcg ataacgccat tctcgctcac gaacgtctgt
     9061 caattgttga cgttaacgcg ggggcgcaac ctctctacaa ccaacaaaaa acccacgtac
     9121 tggcggtaaa cggtgaaatc tacaaccacc aggcattgcg cgctgaatat ggcgatcgtt
     9181 atcagttcca gacagggtct gactgtgaag tgatcctcgc gctgtatcag gaaaaagggc
     9241 cggaatttct cgacgacttg cagggcatgt ttgcctttgc cctgtacgac agcgaaaaag
     9301 atgcctacct gattggtcgc gaccatctgg ggatcatccc actgtatatg gggtatgacg
     9361 aacacggtca gctgtatgtg gcctcagaaa tgaaatcgct ggtgccagtt tgccgcacga
     9421 ttaaagagtt cccggcgggg agctatttgt ggagtcagga cggcgaaatc cgttcttatt
     9481 atcatcgcga ctggttcgac tttgatgcag tgaaagataa cgtgaccgac aaaaacgagc
     9541 tgcgtcaggc actggaagat tccgttaaaa gccatctgat gtctgatgtg ccttacggtg
     9601 tgctactttc tggtggtctg gattcctcaa ttatttccgc tatcaccaag aaatacgcag
     9661 cccgtcgcgt ggaagatcag gaacgctctg aagcctggtg gccgcagtta cactcctttg
     9721 ctgtaggtct gccgggttca ccggatctta aagcagccca ggaagtggca aaccatctgg
     9781 gcacggtgca tcacgaaatt cacttcactg tacaggaagg tctggatgcc atccgcgacg
     9841 tgatttacca catcgaaact tatgatgtga ccactattcg cgcttcaaca ccgatgtatt
     9901 taatgtcgcg taagatcaag gcgatgggca ttaaaatggt gctgtccggt gaaggttctg
     9961 atgaagtgtt tggcggttat ctttacttcc ataaagcgcc caacgctaaa gaactgcatg
    10021 aagagacggt gcgtaaactg ctggccctgc atatgtatga ctgcgcgcgc gccaacaaag
    10081 cgatgtcagc ctggggcgtg gaagcacgcg ttccgttcct cgacaaaaaa ttccttgatg
    10141 tggcgatgcg cattaacccg caggataaaa tgtgcggtaa cggcaaaatg gaaaaacaca
    10201 tcctgcgtga atgttttgag tcatacctgc ccgcaagcgt ggcctggcgg cagaaagagc
    10261 agttctccga tggcgtcggt tacagttgga tcgacaccct gaaagaagtg gcggcgcagc
    10321 aggtttctga tcagcaactg gaaactgccc gcttccgctt cccgtacaac acgccgacct
    10381 caaaagaagc gtatctgtac cgcgagatct ttgaagaact gttcccactt ccgagtgccg
    10441 ctgagtgcgt gccgggcggt ccttccgtcg cgtgttcttc agctaaagcg atcgaatggg
    10501 atgaagcgtt caaaaaaatg gacgatccgt ctggtcgtgc ggttggcgtt caccagtcgg
    10561 cgtataagta agaaatgcgg tactgccccg gagaaatccg gggctgtttt agttattgcg
    10621 ctgcttgttg attctgatat gcagtaagga aatgttccgg gtttgccaca tcactgtttg
    10681 acaactcatc actgtaggcc agtatttcat agattaattt cccaccacta ttgttgaatt
    10741 ccgaatttca tcctgtacga caaaagaatg gctgtagggt ggggaaaaaa cacaaagtaa
    10801 acaataattg acgaatatag cgccacgctg ttcgcaacct aaccaaacag tcactttcga
    10861 gcaattttcc ttgaaaaaga ggttgacgct gcaaggctct atacgcataa tgcgccccgc
    10921 aacgccgata aggtatcgcg aaaaaaagat ggctacgtag ctcagttggt tagagcacat
    10981 cactcataat gatggggtca caggttcgaa tcccgtcgta gccaccatct tttttgcggg
    11041 agtggcgaaa ttggtagacg caccagattt aggttctggc gccgcaaggt gtgcgagttc
    11101 aagtctcgcc tcccgcacca ttcaccagaa agcgttgtac gg
//
LOCUS       DADLSA010000124        11129 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.124, whole genome shotgun sequence.
ACCESSION   DADLSA010000124 DADLSA010000000
VERSION     DADLSA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.124"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            4..435
                     /locus_tag="JN691_003170"
     CDS             4..435
                     /locus_tag="JN691_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244540.1"
                     /translation="MTMNLHTPTTAPKAAPATSVSPLEQSGSPKTKFPEIKTVKTAVV
                     KLISDIEGNLDQRLVYREAVEGLCQEAANRITQCMHSRSTGQTLRDLRELLSVTLDTI
                     IVVEFEGEQTRLNYGGRSAEDVIAMLKALHLEGRKAWLEVE"
     gene            complement(467..994)
                     /locus_tag="JN691_003171"
     CDS             complement(467..994)
                     /locus_tag="JN691_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAY7244541.1"
                     /translation="MRKTTLLVLAAALCVPAFATAAPVLTAGFSPSDGRPALEIVLGA
                     INNARQSIDVAAYSFTSKPVATALAGANRRGVAVRVVADEKANSDRYTAVTYLINQGV
                     PVHLNGRYAIMHNKFMVIDGKNVQTGSFNYTASAVSRNAENVLLIEDAPQLAEAYQRE
                     FNRLWDEGIPLNALY"
     gene            complement(987..1733)
                     /locus_tag="JN691_003172"
     CDS             complement(987..1733)
                     /locus_tag="JN691_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HAY7244542.1"
                     /translation="MLISSYQERYARSTEKIRVLLNFLKEETYSDFQIIKLLFDFKSD
                     RPLYRLLEKVEKMGLIQKHVHESRASRISLWGITTDGLAVILTADDNIMPARFEPYRL
                     TGWSLDHHLDNQLVRLTLEKNGATGWINGDRSTFLSQYSVKHRPDGLISLPDGRLIAV
                     ETERRIKTKARYQSIMASHLLAISDKKWRYAFYVAPDEAKKRALKIIFDSIASVIVKN
                     QHVQLEAKHRDVFRFYTLDELKNLELDNYA"
     gene            complement(1748..3655)
                     /locus_tag="JN691_003173"
     CDS             complement(1748..3655)
                     /locus_tag="JN691_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAY7244543.1"
                     /translation="MIFDKLNHLLSPIANAVLAFIAFLQAHQLALALLSGVMLPFIFS
                     AKHGGQEKPTFLQRILILLSMICFVFGSIAPVAVWFLQRIYGREDIASPPLLTWTMAL
                     LFTVAGFILHVVLRRLISPEFDKVKCGMVKKTSMERDRRTDVRRVKEILPVTTEYDPM
                     DYIDLNKGIFIGLSREGEPQYIPLKEWQTQHADIIGTTGAGKGVVTGILLYQSILADE
                     GVFVLDPKNDEWAPHLYKKACADAGKPFVLIDLNKQEYQMNLIDGITADHLEELFVAG
                     FSLAEKGEAADFYRIDDRKAARTAAQFVKDNPGATVRDIYNGDYVQGIGETIKAFHGK
                     TEELAMLNSINAPGGFALKEIFDKGGCCYIIGSMRNSKIITAQRMILVRLFQLAELRD
                     RVAGNIRPIAIFKDEVKYHLSKPALEGLGAARDKGVHILMAHQSIADLRDCPADLNGD
                     AVVGAVVENCKFKLVYKLQDPDTAEWIARMSGTILVDDESRKVTTTAVLTEKVDNERM
                     LRQAESYYIDSNMLQNLPKFVSYIFSQNALAFPSLISPLKVKKAPLELFTLPPVAVAT
                     VAEVKPVLDLDFDKTAPAVPAPDIIPVSATTTKKPSATPIPTPPVQPDEEWLMASQPA
                     PNDEIHSLLDL"
     gene            complement(4252..4644)
                     /locus_tag="JN691_003174"
     CDS             complement(4252..4644)
                     /locus_tag="JN691_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HAY7244544.1"
                     /translation="MNRIQDELTSERALKKFLRESDFAFLEMLSARISAALDEKRAEH
                     IEQEKLRRQREEKRNELLALIESEGFSPTELVSTTPAKKSAKRKAKYQYFENGVHKKW
                     SGVGRVPVVIQQELDAGKPLESFLITTD"
     gene            complement(4641..6842)
                     /locus_tag="JN691_003175"
     CDS             complement(4641..6842)
                     /locus_tag="JN691_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAY7244545.1"
                     /translation="MRLWIAEKPAVASDIAKALGGNFTRHDGYAESATDIVSYCVGHV
                     LEMVPPETINPAYATWSLDTLPLKLYPVQLQPKASVAKQANTLVKLIRRPDIKMVVHC
                     GDPDDEGQLLVDEVLEFAGNTAPVKRALINDNTAPAVKKAINSLRDNSDFRGMYLKAL
                     MRSAGDAIFGFSMSRCYTLKARDKGYKGTISVGRVQTPVLGMIVRRWRDNQAHSEAFY
                     YQLAGQFISGTDVVCARWQTSEYAPVDDKKRLTDKAWGEGLARALAGKPASVLAAATD
                     RGKTTAAPLPFNLLRLQVYMNRRHKLTAQQTLDITQKLKDSKYITYNRSDCSYLSDEQ
                     FNDAPQVVAALKTLDVFNGLTTDTAQKSAAFDSKKVTAHTAIIPTTNMPDLSRLTDKE
                     KAVYLTIAQFYLAQFVAKKRYDESIAEIKCGDEMFKVSARKITDAGFTTFLNDAEEDD
                     EEDENSTSFEAISRLQTGATLTCREVVISEKKTKPLPLFTEATLLAALVRVADFVADP
                     RIKKLLKEKDKDKKDEHGGIGTPATRAAIIETLKTRDYITVEKGKFIPTAAGLALIDA
                     LPDSVTQPDMTAVWAEKQAAVETGEMTIERFIDGLYAEVSRLVESADIAISATEKHPV
                     KTALNRLAVKCPSCGSELVMTPKTCACTGCQFKIWLEVRGKKLTEKQAEKLIGKGKTD
                     VIKGFKSKKNDDTYSMIVTLKNAQTGELGFEYPPRDPLKGLNVSMPKELKL"
     gene            complement(6839..7147)
                     /locus_tag="JN691_003176"
     CDS             complement(6839..7147)
                     /locus_tag="JN691_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244546.1"
                     /translation="MATKTGFIAPPWPHVRGDSVSAASLISRIEKEAHRGCGLHYEIY
                     QYRALCELHDVLTSLSVRDAETFMTVAADHGFSLSDNDFQESRMMYFETMDEIRRDQE
                     "
     gene            complement(7123..7458)
                     /locus_tag="JN691_003177"
     CDS             complement(7123..7458)
                     /locus_tag="JN691_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244547.1"
                     /translation="MINQTVSINNFSGLLKDEELPAGDLQTVLLVLSEISQKQDHFQA
                     NTSELVQLAAQHSPTFQALPAERQSFFRSVLSMPVFFYTVPLSDDEAAEWEKLNDKDA
                     LQWQQKPDL"
     gene            complement(7485..7802)
                     /locus_tag="JN691_003178"
     CDS             complement(7485..7802)
                     /locus_tag="JN691_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244548.1"
                     /translation="MKHTVITAALLCALTSPAFAADGQNADPNDPCAMVLCLAGKLDG
                     SSPAECDPMYKNFMSIKKKKKGSFLPDHTAAARQKKLSACPSADSAIVGKIISKFGRM
                     NGW"
     gene            complement(7893..8294)
                     /locus_tag="JN691_003179"
     CDS             complement(7893..8294)
                     /locus_tag="JN691_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244549.1"
                     /translation="MMLPKLTGLTAIVVLLAGCSSPPEPAAVDWSQPAQPVTTTRPVW
                     EPNRLIVASPVTEGHWSQVLTRFEPGGIYPAAQWYAVAHASRAVVNAPDGQRFFAAKT
                     WLREGGYKGVVEFRPRSGCLTCDTSEIVFYR"
     gene            complement(8281..9330)
                     /gene="virB11"
                     /locus_tag="JN691_003180"
     CDS             complement(8281..9330)
                     /gene="virB11"
                     /locus_tag="JN691_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HAY7244550.1"
                     /translation="MDDKLTDTPSEGNVALSHLRDLLFGDYLSREGLTEIAINRPGEL
                     HTKINGAWEMHASPVTLRQCSSFARALAVWNNDTIDETSPILSATLPTGERCQIVVPP
                     ACERNTISITLRKPDYFQRTHQSYIDAGFYDRVQGTEKVESKDGELSRIYLSGNIPLF
                     MEKCVEYGKTLFVVGETGSGKTTYMKMLLHYIPETLRLITIEDNPELKFYSQKNYVHL
                     FYPADAGEGAIVTSDRLVRAIYRMNPDRPLLAEVRGREAWDALKLIESGHEGFMSSLH
                     AGSPRECISGLIDRCYEHPACRGMPVDVLLRKVLHCVDVIVSVDVHGNVRRMSDVYFK
                     PLHMQAMKESFNDAA"
     gene            complement(9334..10530)
                     /locus_tag="JN691_003181"
     CDS             complement(9334..10530)
                     /locus_tag="JN691_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HAY7244551.1"
                     /translation="MMNEQEDIKTAAELEAEARERARAEIDGDKPAAGAVPPGQPQVV
                     QHAKRRSRRSLVLAAISLVLLVIIATGGDYLMAKLKGDGSGKSASHEPESHATSPARE
                     RNNLGRDLNPLGQLADTTGQEKTTPTDTAAPPQASPPPLTFDKVSALADSAPTTASSA
                     PKSTSSSNVSPAGSASAPGSATPAGQSQDNGLARITGVKRLGLDPDLYIPADRHIPCA
                     MMQRFVSDVGGRFNCLISEDIYSASNAVRLIPAGTEARGLYRTGTLKNGQGRLFLAIT
                     ELRTPEPGRLVIPMVDSQAVGALGENGVAGWIDNHWLERIGNTLLLGTVQDFAAAASG
                     SSPGKDRNTDYTENTRAATAEMAKTLLENSINIPPTMYLNQGDVIGLVTGADIDFSDV
                     YRLRMR"
     gene            complement(10527..>11129)
                     /locus_tag="JN691_003182"
     CDS             complement(10527..>11129)
                     /locus_tag="JN691_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HAY7244552.1"
                     /translation="GKTVQKIFTPTQQDWQTNLFVTTTKRDYSLMLNVIDNDREAGNL
                     AFVVRFSYPDDTRRQNDAARLARQQELQAAQEKERIQFALKKSTAPRNWNYTRRVAAG
                     SESITPDFAYDDGRFTYLGFSPQKLIPSPTVIVGGSEQVITPTFSTEGNYRVMVLRSL
                     SPRFVLRYGSQVVGIENGSYGKVTVANGSTVSPDVKLESK"
BASE COUNT         2201 a         3170 c         3172 g         2586 t
ORIGIN      
        1 accatgacga tgaacctgca caccccgaca accgccccta aagctgcacc ggcgaccagc
       61 gtatccccgc tggagcagtc aggctcccca aaaacgaaat ttcctgaaat caaaaccgtc
      121 aaaacggcgg tggttaaact gatcagcgac atcgagggca acctcgatca gcgtctggta
      181 tatcgcgagg ccgtagaggg attgtgccag gaggcggcca accggataac ccagtgcatg
      241 cattcacgca gtacagggca gacgctacgt gatctccgcg agctgctgtc ggtgacgctg
      301 gacaccatta tcgtggtgga gtttgaaggg gagcagaccc gtctcaacta tggcggacgt
      361 tcagcagaag acgtgatcgc catgctgaaa gcgctgcatc ttgaaggacg caaagcctgg
      421 ctggaagtgg agtaaaaaaa aagccccgtc cgcagacggg gctgattcag tacagggcat
      481 tcagcgggat gccctcatcc cacaggcggt taaattctcg ctggtacgcc tccgccagct
      541 gcggcgcatc ctcgatcagc agcacgtttt ccgcgttgcg cgaaacggcg cttgccgtgt
      601 agttaaacga cccggtctgg acgtttttgc cgtcgatcac cataaacttg ttgtgcatga
      661 tggcgtaacg gccgttaagg tgcaccggca cgccctggtt tatcagatac gtcacggcgg
      721 tgtagcggtc gctgttcgcc ttttcgtcgg ccaccacgcg cacggccacg ccgcgccggt
      781 tagcaccggc gagcgccgtc gcgaccggct tactggtgaa gctgtaggcc gcgacgtcaa
      841 tgctctgcct ggcgttgttt atggcgccga gcacgatttc aagagcaggg cgcccgtcag
      901 acggcgaaaa acccgccgtc aggacgggcg cggcggtggc gaacgcgggc acgcagaggg
      961 cggcggccag caccagaagg gtggttttac gcatagttgt ccagctccag gtttttcagt
     1021 tcatcgaggg tgtaaaagcg gaaaacgtcg cggtgttttg cctccagttg cacatgctgg
     1081 tttttcacga tcacgctggc gatgctgtcg aaaataatct tcagggcgcg ctttttcgcc
     1141 tcgtccgggg cgacgtaaaa cgcgtaccgc cattttttat cgctgatagc cagcaggtgg
     1201 ctcgccatga tggactgata gcgcgccttt gttttgatac gtcgttcggt ttcaacggcg
     1261 atcagcctgc cgtccggcag gctgataagc ccgtcgggac ggtgtttgac gctgtactga
     1321 ctgaggaacg ttgaacggtc gccgttaatc caccctgtcg cgccgttctt ctccagcgta
     1381 aggcgcacta gctggttatc aaggtgatgg tcgagtgacc agccggtcag gcggtagggt
     1441 tcaaagcggg cgggcattat gttgtcgtcc gccgtgagga taacggccag cccgtcggtg
     1501 gtaatgcccc acagggatat tctcgacgcg cgggattcat gcacgtgctt ctgaatcagc
     1561 cccattttct ccaccttctc caacaaccgg tagagagggc ggtcagattt aaaatcaaaa
     1621 agcagcttta tgatttgaaa atcgctgtag gtttcctcct tgagaaaatt cagcagtacg
     1681 cgaatttttt cagtgctgcg tgcataacgt tcctgatacg acgaaataag catagaaact
     1741 ccttatttta taaatccagc aacgaatgta tttcgtcatt tggagcgggc tgtgatgcca
     1801 tcagccactc ctcgtcaggt tgcaccggcg gagtgggtat tggcgttgct gagggttttt
     1861 tggtggttgt tgcgctgacc ggaataatgt caggtgccgg aactgccgga gccgttttat
     1921 caaaatccag atccagcacc ggtttaactt ctgccaccgt agcgactgcg accggcggca
     1981 gggtaaacag ctccagtggg gcttttttaa ccttgagcgg tgagattaac gacgggaacg
     2041 cgagcgcgtt ctggctgaag atatagctga cgaacttcgg caggttctgg agcatattgc
     2101 tgtcgatgta atagctttcc gcctgccgaa gcatgcgctc gttatcgact ttttctgtca
     2161 gcaccgccgt ggtggtgact ttccggcttt catcatcgac taaaatcgtg cccgacatgc
     2221 gcgcaatcca ttcggcggtg tctggatcct gaagtttata taccagctta aacttgcagt
     2281 tttccacaac cgccccaacc acggcgtcgc cgtttaaatc cgccggacag tcgcgcaggt
     2341 cggcaattga ctggtgcgcc attaatatat ggacgccttt atcgcgtgcc gcgcctaacc
     2401 cttcgagcgc cggtttggac aggtgatatt tcacctcgtc cttaaagatg gcgatggggc
     2461 ggatattccc cgcgacgcgg tcgcgcaatt ccgcgagctg gaacaggcga actaaaatca
     2521 tgcgctgcgc ggtaatgatt ttagagttac gcatcgaacc gatgatatag cagcagccgc
     2581 ccttgtcgaa aatctccttc agcgcaaaac cgcctggcgc gttaatgctg ttgagcattg
     2641 ccagctcctc ggttttaccg tgaaaggctt taatcgtctc gccaatgccc tgaacataat
     2701 cgccgttata aatatcccgc acggttgcgc ccggattatc cttcacgaac tgcgccgccg
     2761 ttctcgccgc cttgcggtcg tcgatacggt aaaaatccgc cgcctcgcct ttttccgcca
     2821 gactgaaacc ggccacgaat aattcctcca gatggtcggc ggtgataccg tcgataaggt
     2881 tcatctggta ttcctgttta ttcaggtcga taagcacaaa gggttttccg gcgtccgcgc
     2941 acgctttttt atacaggtgc ggtgcccact cgtcgttttt ggggtcgaga acaaaaacgc
     3001 cctcgtcggc gaggatgctc tgataaagca gaataccggt tacgacgcct ttaccggcac
     3061 cagtggtgcc gataatgtcg gcgtgctggg tctgccattc ttttaacgga atatattgcg
     3121 gctcaccctc gcggctcagc ccgataaaaa tccccttatt cagatcgatg taatccatcg
     3181 gatcgtattc tgtggtgacg ggtaaaattt cttttacccg gcgaacgtcg gttctgcggt
     3241 cgcgctccat gctggttttt ttcaccatgc cgcatttgac cttatcaaat tcaggcgaga
     3301 tcaaacgccg taaaaccacg tgcagaataa aaccggcgac ggtaaacagt agcgccattg
     3361 tccaggtaag cagcggcgga ctggcgatat cttcccggcc ataaatgcgc tgtaaaaacc
     3421 agacggccac tggcgctatc gagccgaaaa caaaacagat catggaaagc agaattaaga
     3481 tcctttgcag aaacgtcggc ttttcctgac cgccgtgttt cgcagaaaat ataaaaggca
     3541 gcatgacgcc ggacagcagc gctaacgcca gttgatgcgc ctgcaaaaag gcgataaagg
     3601 ccaggacggc atttgctatc ggcgacagca aatgattcag tttgtcgaat atcacgatat
     3661 tctccttcac ttaaattcgg tcagcaccat cggtcagtca gaacggtcac aggttgtgat
     3721 cgttctgact gaccgattcc atgctgcaaa agaagtggcc gctaagcggc catgatcgca
     3781 ggaggtacac tcccgcgatc taacggcgtg cggcattacg cgcgggctgg acgcccttac
     3841 tgcatgtcgc atgggggtaa tgtcctggct gaacgcccgg ccaaaacccc caaggtcaaa
     3901 agaggccaca ccgtcgcgcc acgctaccgg cttttggccg gagacttagg cacgacggtg
     3961 tggggggcaa gccccccaaa taaaccgacg cgtgggcgcg tctttttgct cgctggggcg
     4021 gcaaaattta ttcggccccc cccggctttt ttcggctcac ttggggtgag cgctcaaaaa
     4081 gtggaatcgg cgacgcaaat tagcaatttc ttcgaaattg aatttactcg cctcctgcca
     4141 cttcgcagac tcaggcggcc tgtcgattag caattttttc aaaattgaat cgccatgctc
     4201 gccagtcttc gaagcccgaa aagccaggtc aaaaccgacc ctgtgccgtt gttagtcggt
     4261 ggtaataagg aaggattcaa gaggttttcc ggcgtcaagt tcttgctgaa ttacaaccgg
     4321 cacgcgtccg acgcctgacc attttttatg cacgccgttt tcaaaatact gatatttagc
     4381 tttgcgtttt gccgactttt tggcgggagt tgtactaacc agctctgtcg gtgaaaagcc
     4441 ctccgattca atcagcgcca gcaattcatt gcgcttttct tcccgctgtc ggcgtagctt
     4501 ttcctgctcg atatgctctg cgcgtttttc atcaagcgct gctgaaatac gtgcactcag
     4561 catctccaga aaggcaaaat cactttcacg taaaaacttt ttcagcgcgc gctctgaggt
     4621 taattcgtcc tggatacggt tcataatttc agctccttcg gcatagatac gttcaatcct
     4681 ttcagcggat cgcgtggtgg atattcaaaa ccgagttccc cggtttgcgc gtttttcagc
     4741 gtgacgatca tcgaataggt gtcgtcgttt ttcttcgatt taaaaccttt aataacgtcg
     4801 gttttgccct tgccgattaa cttctcggcc tgtttttccg tcagcttttt gccgcgcact
     4861 tccagccata ttttgaactg acagcccgta caggcgcagg ttttcggcgt cataaccagc
     4921 tcagaaccgc acgacggaca tttaaccgcg aggcggttaa gcgccgtttt aacgggatgt
     4981 ttttccgtcg cgctgatggc aatatcggcg ctctcaacca ggcgggaaac ttccgcgtaa
     5041 agaccatcaa taaagcgctc gatggtcatt tcgccggttt caaccgccgc ctgtttttca
     5101 gcccatacgg cggtcatatc cggctgcgtc accgagtcgg gcagggcatc aattaacgcc
     5161 agcccggcgg cagttgggat aaatttgccc ttttccacgg tgatgtaatc gcgggttttc
     5221 agcgtctcaa taatagccgc gcgggtcgcc ggtgtgccga tgccgccgtg ttcatctttt
     5281 ttgtctttat ccttctcctt gagcaatttt ttaatgcgcg gatcggcgac aaaatcggcc
     5341 acgcgaacga gggcagcgag cagggtcgcc tcggtaaaca gcggcagcgg tttagttttt
     5401 ttctccgaaa tcaccacctc gcggcaggtt aatgtcgccc cggtttgcag gcggctgatg
     5461 gcctcaaacg aggtgctgtt ttcgtcctcc tcgtcgtctt cttccgcgtc gttcaggaac
     5521 gtcgtaaaac ccgcatcggt gattttacgc gccgagactt taaacatctc gtcaccgcat
     5581 ttaatctccg cgatcgactc gtcatagcgc tttttcgcca cgaactgcgc gagataaaac
     5641 tgcgcgatgg tgagataaac cgccttttcc ttgtcggtaa ggcggcttaa atccggcatg
     5701 ttcgtcgtcg ggataatggc ggtgtgggcg gtgacttttt tactgtcaaa cgccgcactt
     5761 ttttgcgccg tgtcggtggt aaggccgtta aaaacgtcga gcgtttttaa ggccgccacc
     5821 acctgtggcg cgtcgttgaa ttgttcatcg gacagataag aacagtcaga gcggttgtag
     5881 gtaatgtatt tactgtcctt gagcttctgc gtgatatcga gcgtctgctg cgcggtcagt
     5941 ttgtgcctgc ggttcatata cacctggagg cgcagcaggt taaacggcag cggtgcggcc
     6001 gtggtttttc cccggtcggt ggcggccgcg agcacgctgg ccggttttcc ggccagagcc
     6061 ctcgcgagtc cttcccccca ggccttatcc gtcagccgct ttttatcgtc caccggcgcg
     6121 tattcggacg tctgccagcg ggcgcagaca acgtccgtac cgctgataaa ctgccctgcg
     6181 agctggtagt aaaacgcctc gctgtgcgcc tgattgtcgc gccaccggcg cacaatcatt
     6241 cccagcaccg gcgtctggac gcggccaacc gaaatcgtgc ccttatagcc cttatcgcgg
     6301 gctttgaggg tgtaacagcg gctcatactg aagccaaaaa tcgcgtctcc tgcgctgcgc
     6361 atcagggctt tcaggtacat cccacgaaaa tcgctgttat cgcgcaggga gttgatggct
     6421 tttttcaccg ctggcgcggt gttgtcgtta ataagcgccc gtttcaccgg cgcggtgttt
     6481 ccggcgaact ccagcacctc gtcaaccagg agctgcccct cgtcgtccgg gtcgccgcag
     6541 tggacaacca ttttgatatc cgggcgccga atcagcttca ccagggtatt agcctgcttt
     6601 gccacgctcg cttttggctg aagctgtacc gggtacagct tcagcggcag ggtgtcgagc
     6661 gaccaggtgg cgtaggccgg gttgatcgtc tccgggggca ccatttccag cacgtggccg
     6721 acgcagtagc tcacgatatc ggtcgccgat tcggcatagc cgtcgtgccg ggtgaagttg
     6781 ccgcccagcg ctttcgcgat atcgctcgcg actgcgggtt tttctgcaat ccacagtctc
     6841 attcttgatc tctcctgatt tcatccatcg tttcgaagta catcattcga ctttcctgaa
     6901 agtcgttatc agacagacta aagccatgat ccgccgctac tgtcatgaac gtctcagcat
     6961 cacggacgct gagactggtc agaacatcgt gcaattcgca cagggcgcga tattggtaga
     7021 tttcgtaatg caggccgcag ccgcgatgcg cttctttctc gatgcgagaa atcaggctcg
     7081 cggcactaac ggaatcacca cgcacatgcg gccacggcgg ggctataaat ccggtttttg
     7141 ttgccattgg agtgcatcct tatcgttgag tttttcccat tccgccgcct cgtcgtcgct
     7201 cagcggaacg gtgtaaaaga agaccggcat actgagcacg gagcggaaga acgactgacg
     7261 ttctgctggt aatgcctgga aggtcggcga gtgctgcgcg gcgagctgga cgagttcgga
     7321 cgtgttggcc tgaaaatgat cctgtttctg gctgatttcc gacaaaacga gcagcaccgt
     7381 ctgcaaatca cctgcaggaa gctcctcatc tttcagcagc ccggaaaagt tattaataga
     7441 aacggtttga ttaatcacgt ggtgtctcct ttacggctgc cgttttacca accgttcata
     7501 cggccaaatt tcgagatgat cttgccgaca atggcgctgt cagcggacgg gcaggcgctc
     7561 agttttttct gccgcgctgc ggcggtgtga tcgggtaaaa acgacccctt ttttttcttt
     7621 ttgatgctca tgaaattttt gtacatcggg tcacactcgg cgggactgct gccgtcgagc
     7681 tttccggcca ggcacagcac catcgcgcac gggtcattcg ggtcggcatt ctggccatcg
     7741 gcggcgaacg ccggagaagt aagggcgcac agcagcgccg ccgtgattac ggtgtgtttc
     7801 atgatgattt cctctgaagg gaagcagggc gctaacgccc gttggttgtt caacgtcggt
     7861 gacggtctga aggactcaga ccgtcaccga cgttatcggt agaagacgat ttctgaggtg
     7921 tcgcaggtca ggcagccgga gcgggggcga aactccacca cacccttgta gccgccctcg
     7981 cggagccagg ttttggccgc aaagaagcgc tggccgtccg gggcatttac taccgcgcgc
     8041 gaggcgtgcg caaccgcgta ccactgtgcg gccgggtaaa tcccgccggg ttcaaaacgc
     8101 gtaagcacct gcgaccagtg cccttccgtc accggcgagg ccacgataag acggttgggt
     8161 tcccataccg gccgggtggt cgttaccggc tgcgccggtt gtgaccagtc aacggccgca
     8221 ggctccggtg gcgaagaaca gcccgccagg aggacgacga tcgccgtcag gccggtcagt
     8281 ttaggcagca tcattgaaac tctccttcat agcctgcatg tgcaggggtt taaaatagac
     8341 gtcgctcatg cggcgcacgt tgccgtgaac gtccacgctc acaatgacgt cgacgcagtg
     8401 cagtaccttg cgcagcagca cgtcaaccgg catgccccgg cacgccggat gctcgtagca
     8461 gcggtcaatc agcccggaga tacactcgcg cgggctgccc gcgtgcaggg aggacataaa
     8521 cccctcgtgc ccggactcaa tcagcttgag tgcgtcccac gcctcgcggc cacgcacctc
     8581 ggcgagcagg gggcggtcgg ggttcatacg gtaaatcgcg cgcaccaggc gatcagaggt
     8641 gactattgcg ccctctccgg cgtccgccgg gtagaacaga tgaacgtagt ttttctggct
     8701 gtaaaacttc agctccgggt tgtcctcgat ggtgataagg cgcagcgtct cgggaatgta
     8761 gtgcagcagc atcttcatgt aggtggtttt cccagagcct gtttccccca ccacaaagag
     8821 cgttttgccg tactccacgc acttttccat aaaaagcggg atattcccgg acaggtatat
     8881 gcggctcagc tccccgtctt ttgattccac cttttctgta ccctgcaccc ggtcataaaa
     8941 cccggcatcg atatacgact gatgggtgcg ctggaagtag tccggcttac gcagggtgat
     9001 tgagatggtg ttacgctcac aggccggggg caccactatc tggcagcgtt ctcctgtagg
     9061 gagcgtggcc gagaggatag gcgacgtttc atcaatggtg tcgttgttcc acacggccag
     9121 cgccctggca aaggacgagc actggcgcag cgtgaccggc gaggcgtgca tttcccacgc
     9181 cccgttgatt tttgtgtgca gctctccggg gcggttgatg gcaatttccg tcagcccctc
     9241 ccggctcagg tagtccccga acaacagatc gcgcaggtgt gacagcgcga cgttgccctc
     9301 tgacggggta tcagtcagtt tatcgtccat cgcttacctc atgcgcagtc tgtagacgtc
     9361 tgaaaaatca atgtccgccc ccgtgaccag gccaatcacg tcaccctggt tgaggtacat
     9421 cgtcggcggg atgttgatgc tgttttccag gagcgttttt gccatttccg ccgtggccgc
     9481 gcgggtgttc tccgtgtagt ccgtgttgcg atccttgccc ggcgacgagc cggaggccgc
     9541 agcggcaaag tcctgtaccg tgccgagcag gagggtatta cctatccgct ccagccagtg
     9601 attgtctatc cagcccgcga cgccgttctc gccaagcgcc cccacggcct ggctgtcaac
     9661 catcggaata accaggcggc cgggttccgg cgtgcgcagc tcggtgatgg cgaggaacag
     9721 acgcccctgc ccgtttttca gcgtgccggt gcggtacagg ccgcgcgcct cagtgcctgc
     9781 cgggataagg cgcacggcgt tgctggcgct gtaaatgtcc tcgctgataa ggcagttaaa
     9841 gcgcccgccc acgtccgata caaagcgctg catcatcgcg caggggatat gccggtcggc
     9901 cggaatgtac aaatccggat cgaggccgag ccgcttcacg ccggtaatgc gcgccagccc
     9961 gttatcctgc gactgcccgg cgggtgttgc agagcctggc gcggacgcgc tgcccgcagg
    10021 tgatacgtta ctgctggacg tgcttttcgg ggcgctgctt gcagtggttg gggcgctgtc
    10081 tgccagggcg ctgaccttgt caaacgtcag ggggggcggt gatgcctgcg gcggcgcggc
    10141 ggtatcagtc ggagtagtct tctcctggcc ggttgtgtct gccagctgtc ccagcgggtt
    10201 cagatcccgt ccgaggttat tgcgctcgcg ggcgggggag gtggcgtgcg attccggttc
    10261 atggctcgcg ctttttccgc tgccgtcacc cttcagtttc gccatcaggt aatccccgcc
    10321 ggtggcgata atgaccagta acaccaggct gatggcggcc agcacgagcg agcggcggct
    10381 gcggcgcttc gcgtgctgca ccacctgtgg ctgtccgggt ggcaccgcgc ctgcggcggg
    10441 tttatcgccg tcaatctctg cccgcgcccg ctcgcgggct tccgcctcca gttcggcggc
    10501 ggttttaatg tcttcctgtt cgttcatcat ttgctctcca gtttcacatc cggtgagacg
    10561 gtgctgccgt tcgccacggt gactttcccg taactgccgt tttcaatccc cacgacctgg
    10621 ctgccgtagc gcagcacaaa gcgtggagaa agagagcgca gtaccatcac ccggtagtta
    10681 ccctcggtgc tgaaggtcgg ggtgatcacc tgctcgctgc cgccgacgat gacggtcggc
    10741 gacgggatca gcttctgcgg tgaaaagccg agataggtaa accggccgtc gtcatacgca
    10801 aaatccgggg tgatcgactc cgaacctgcc gccaccctgc gggtgtagtt ccagttgcgc
    10861 ggcgcagtgg atttcttcag ggcaaactga atgcgttctt tctcctgcgc ggcctgaagc
    10921 tcctgctgac gggcgaggcg ggctgcgtcg ttctgcctgc gggtatcgtc ggggtagctg
    10981 aagcgcacca caaaggccag atttcccgcc tcgcgatcgt tatcgatcac gttcagcatc
    11041 aggctgtagt cgcgctttgt ggtggtcaca aacaggttgg tctgccagtc ctgctgcgtg
    11101 ggggtgaaaa tcttctggac ggttttgcc
//
LOCUS       DADLSA010000125        10910 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.125, whole genome shotgun sequence.
ACCESSION   DADLSA010000125 DADLSA010000000
VERSION     DADLSA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.125"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..208
                     /locus_tag="JN691_003183"
     CDS             <1..208
                     /locus_tag="JN691_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY7244553.1"
                     /translation="LKEWNARQLRPRNPETVRRWVRECRIFPPPVKDGREYLFHESAV
                     KVDLNRPVTGSLLKRIRNGKKAKS"
     gene            186..1259
                     /locus_tag="JN691_003184"
     CDS             186..1259
                     /locus_tag="JN691_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7244554.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     VSEAIQANIELLSENRRESLIDRIKGADAITLHAWLDRYETILSERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLNDISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYIEIYHAAEPLPIWLRLAMDLAVITGQRVGDLCR
                     MKWSDINDNHLHIEQGKTGAKIAIPLTLTIDALNISLVDTLQKCREASGSETVIASTH
                     HEPLSPKTVSKYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHK
                     SDSMAARYRDSRGREWDKIEIDK"
     gene            complement(1317..1877)
                     /locus_tag="JN691_003185"
     CDS             complement(1317..1877)
                     /locus_tag="JN691_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HAY7244555.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKLHV"
     gene            complement(1874..3562)
                     /locus_tag="JN691_003186"
     CDS             complement(1874..3562)
                     /locus_tag="JN691_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HAY7244556.1"
                     /translation="MSGEKKTKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(3711..6338)
                     /gene="gyrA"
                     /locus_tag="JN691_003187"
     CDS             complement(3711..6338)
                     /gene="gyrA"
                     /locus_tag="JN691_003187"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAY7244557.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYYTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6485..7207
                     /gene="ubiG"
                     /locus_tag="JN691_003188"
     CDS             6485..7207
                     /gene="ubiG"
                     /locus_tag="JN691_003188"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HAY7244558.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPSGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(7335..10910)
                     /gene="yfaL"
                     /locus_tag="JN691_003189"
                     /pseudo
     CDS             complement(7335..10910)
                     /gene="yfaL"
                     /locus_tag="JN691_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2621 a         2976 c         2669 g         2644 t
ORIGIN      
        1 gctcaaagaa tggaacgccc gtcagttacg tccacggaac ccggaaacgg ttcgccgctg
       61 ggtacgcgag tgcaggatat tccctcctcc ggttaaggat ggaagagagt atctgttcca
      121 cgaatcagcg gtaaaggttg acttaaatcg accagtaaca ggtagccttt tgaagaggat
      181 cagaaatggg aagaaggcga agtcatgagc gccgggattt accccctaac ctttatataa
      241 gaaacaatgg atattactgc tacagggacc caaggacggg taaagagttc ggattaggca
      301 gagacagaag gatagcagtt tcagaggcta ttcaggccaa tattgagttg ctatctgaga
      361 acaggcgtga gtcactgata gacaggatta aaggcgctga cgcaatcact cttcatgcgt
      421 ggcttgaccg atatgaaaca atcctcagcg agaggggtat cagaccgaaa actctactcg
      481 actacgccag caaaatcagg gcgattcgaa gaaaattgcc ggacaaaccg ctcaatgaca
      541 tatcaacgaa agaggtggca gcaatgctaa acacctacgt agcagaaggt aaagcggctt
      601 ccgcaaaatt aatcaggtca acccttgttg acgtttttcg tgaagcaata gccgaggggc
      661 atgtggctac gaatccggta acagcaaccc gcacagcaaa gtcagaagta aggcgctcaa
      721 ggcttacagc taatgagtat atcgagattt accatgcagc cgaacctctc cctatctggc
      781 tgaggctggc aatggatttg gccgtcatta cagggcagag agtgggcgat ttatgcagaa
      841 tgaagtggtc agacataaac gacaaccatc ttcacattga gcagggtaaa acaggagcca
      901 agattgccat tccgctgacg ctaacgattg acgcgctcaa catctcactg gttgatacac
      961 tacagaaatg cagggaggcc agcggcagtg aaactgtaat cgcctcaacg catcacgaac
     1021 cgctttcccc gaaaacagta tcgaagtatt ttacaaaggc gagaaatgcc tccggactct
     1081 catttgatgg agacccgcca acattccatg aactgcgtag cctgtcagcg aggctatacc
     1141 ggaaccagat tggcgataaa tttgctcaac gtcttctcgg acataaatca gattcaatgg
     1201 cggcgcggta tagggacagc cgtggacggg aatgggacaa aattgaaatc gacaaatgat
     1261 tttattttga ccaataatga cctgctcatc ttaacagatt gataattaat gcaattttaa
     1321 acatgcaact tccatgtcat aagttgttgc agactgactg cgcgcattcc gttgtcagtt
     1381 ttcgtggcgc ttccggtgtg gtagatgacg taacgcccca tccagaccat taagtgctgg
     1441 gcatcgccct gatcgaaaaa aatcatatcg ccaggcagcg cctggtttat gtcttggcca
     1501 ataaactgac tgttgtactg aatcaaatta atcgcggtca cgtaggggcc gtttttcccg
     1561 ttcccctgat tccagttttg cgccagttga cgctgttcag gtgttagcgt catctctggc
     1621 ggcaaatact ggctagataa accgttactt tttagccatt tactgtcgtg aactttcagc
     1681 gtctcgttcg ccgcaaatcg caccaagccc gcacaatcct gctgatacca gcgtggactt
     1741 ggcccctggc ggagctgttc ttgtgcaatg cgtacaaacc aggcgcgaaa cagcccggat
     1801 tgttcgacat tcagcatttc gctatgggca acaacacaac acagccagca aatcagcgcc
     1861 agcagcccgt gtctcatagc ggctgccagg ttatgggtag ccactgccag gcgacaccgg
     1921 gttccatctg ggccagcttc atgacataac gcggttgttg agataaagcg tccagcttcg
     1981 gcattaataa agtttgtgcg gcgttataaa aaaccggttc gagattcttc ggcagactgg
     2041 tcaacgtttc gttacgcagc agtttcgcca cgccttgtgg attgatatag agcggaacga
     2101 tgccatcagt gggtattaca tccaccattg ccgggcgggt tttattcagt gtttgcaggg
     2161 cgttattaac cagcgtgtca tcgagggaga aaagcagcgt tttgttttgc atcgccagcg
     2221 acacacggaa aaaataatcc gacattaatt gatcgggttg cgccgcctgc gctttcggat
     2281 actgaccgta tcgggaactc acttcgcgac accagatttg cgcttcgccc tgctgagtct
     2341 ggcttaccgg caaaacacct tctggcgctt tgctttcgtg cgcaccaata ttttgcgtaa
     2401 acagtttccc tggcaattgc gcctgttcgg cagtgccatc aaactgaccg acaaacagcg
     2461 gggtttgcaa ttttgagtct tcataccagc acagccccgc ggcaccgtct aacgccccat
     2521 tcagcttgtc gttttcctgg ctgatgtgcg aaagcatctc ttcggcaata ccgtgtgaat
     2581 acggcaccgc cacacagaag ctggctccgg caggcatact gttccagacc ggcgtaaaat
     2641 cgaaactggc atctacgctg gcggattcat catttaacgc cacaaaactg tgccagccgt
     2701 cgttacccat ttcgaagcgt acgccagcaa aagaaggcat taaccgctgg tagccaaaac
     2761 ccagcaacct ggcgctggcc acgatgcgca ggcgtactgg cgttttttcg gcagtacgct
     2821 cttccaggcc aaagctggct tgccagcgtt ttttaccgct caacaaatca cttgcgatcg
     2881 ccgtggcttc ggtatcctgc tgatcatctt taaacaacat atccgtactg gaaaacacca
     2941 gcatcttgtc ctgatatgtc gcgaacatca gggcgttatt gccgttatag cgcaactgat
     3001 aaacaggaat agtttcacta tttatcttga tgctactgat ttccgttttg cttaactggc
     3061 tgtcgctggt agcggcaaac aacaatggct ccagcagttt gcttaaacca ctgcgctgga
     3121 tcagcaccat gtaatgtgaa agatggcctt gtttatcgtg ccatagcgcc gcctgcgcgg
     3181 gctgatctaa gagtgacgaa aagagcttat ctttcagcgt gagatcgtgt tcatagacaa
     3241 tgcggcgaat gctgccttca atgcccagac gatcggcatg attctgataa tagaaaacga
     3301 aatcttcgct cagaacatcg tggagaaacg gaatggtgag gagatctttg ggaagctggc
     3361 tcagagagtc gctgtctaga aagaggtccg gctcattgag atcgatttgc agattgttgt
     3421 gcaccaccag cggcgacaac gttttttctg gcccactgcc agcatattgc aacgcccaga
     3481 cgccagcgga aagcagtgct attgcgccaa aacctacaag accatagaac cgccagcctt
     3541 tcgtcttttt ttcaccactc attgccacat tccttgtgta tagccagcca ttttttacgg
     3601 gcacagccaa actttaccgc gccctaatac gacaaaagcc cagactttgc agcctggact
     3661 tttcaattca aacaagggag atagctccct tttggcatga agaagtaaaa ttattcttct
     3721 tctggctcgt cgtcaacgtc cacttccgga gcgatttcat catccccttc cgcggcactg
     3781 ccgtcgatgg tatccagatc ttcctcgtca accggttcag caacacgctg cagacccact
     3841 acgttttcat cttccgcagt acggatgagg atcacgccct gggtgttacg gcccacgatg
     3901 ctgatttctg aaacgcgagt acgtaccagc gtaccggcat cggtgatcat catgatctgg
     3961 tcgcagtcat ctacctgcac cgcgccaacc actaaaccgt tacgttcggt aaccttgatg
     4021 gagataaccc ctttcgtcgc acgcgacttg gttgggtatt ccgccactgc agtacgttta
     4081 ccgtaaccgt tttgcgttgc ggtgaggatt gcgccatcgc cacgaggcac gatcagagag
     4141 acgactttat cgccttcacc taagcgaata ccgcgcacac cagtggtatt gcagcccatc
     4201 gcacggacag aagactcttt aaagcgcacc actttacctt cggcggagaa cagcattact
     4261 tcatcttcgc cactggtcag atcaacgccg atcagctcat cgccgtcaac cagtttgatc
     4321 gccactttac cggcggtacg cagacggttg aattcggtga ggacggtttt cttcacagta
     4381 ccgttagcgg tcgccatgaa gactttcacg ccttcttcaa actcggtcac cggcaggatc
     4441 gcggtgatac gttcgtcctg ttccagcggt agcaggttga cgatcggacg accgcgcgcg
     4501 ccacgagtgg cttccggcaa ctggtaaact ttcatcgaat agacgcgacc acggctggag
     4561 aagcacagaa tatggtcgtg cgtgttcgcc accagcagtc ggtcgataaa gtcttcttct
     4621 ttaatacgtg cggcagattt acctttcccg ccacgacgct gcgcttcgta ttcagaaagc
     4681 ggctgatatt taacgtagcc ctggtgagag agcgtcacaa ccacatcttc ctgggtgatc
     4741 agatcttcca ggttgatgtc tgcgctgttg gcggtgattt cagtacgacg tttgtcaccg
     4801 aactgttcac gaaccagctc cagctcttcg cggatcactt ccatcagacg atcggcgcta
     4861 ccaagaatac gcaacagttc cgcgatctga tccagcagct ctttgtattc gtcgagcagt
     4921 ttttcgtgct caaggccggt cagtttctgc aaacgcagat ccagaatcgc ctgagcttgc
     4981 tgttcggtca ggtagtacag accatcacgc acgccgaact ctggctccag ccattccgga
     5041 cgcgcagcat cgtcgccagc acgttccaac atggcggcaa cgttgcccag ctgccacgga
     5101 ttagcaacca gcgcagtttt cgcttctgca ggcgtcggcg catgacggat cagttcgatg
     5161 atcgggtcaa tgttcgccag cgccacggct aatgcttcaa ggatatgagc acgatcgcga
     5221 gctttacgca gttcgaaaat agtacgacgg gtcaccactt cacggcggtg acgaacaaac
     5281 gccgcgatga tgtctttcag gttcatgatc ttcggctgac catggtgcaa tgccaccatg
     5341 ttgataccga aagaaacctg caactgggtc tgggagtaga ggttgttgag cacaacttca
     5401 ccgaccgcat cgcgtttcac ttcaatcacg atgcgcatac cgtctttgtc agactcgtca
     5461 cgcagcgcgc tgatgccttc cacgcgtttt tcttttacca gttccgcaat cttctcgatc
     5521 aggcgcgctt tgtttacctg atacggaatt tcgtggacga taatggtttc acgtccggtt
     5581 ttggcgtcaa cttccacttc agcacgggcg cggatatata ccttgccgcg accggtacgg
     5641 taagcttctt caataccgcg acgaccgtta atgattgccg ccgtcgggaa gtccggcccc
     5701 gggatgtgtt ccatcagccc ttcaatgctg atgtcttcat catcgatata cgccagacaa
     5761 ccgttgatga cttccgtcag gttgtgcggc gggatgttgg ttgccatacc tacggcgata
     5821 ccggaagaac cgttcaccag caggttagga attttggttg gcatgacgtc gggaattttt
     5881 tccgtgccgt catagttatc aacgaaatcg accgtctctt tttcgagatc ggccatcagt
     5941 tcatgggcaa ttttcgccag acggatttcc gtataacgca ttgccgccgc agagtcgccg
     6001 tcgatggaac cgaagttacc ctgaccgtct accagcatgt aacgcagcga gaatggctgc
     6061 gccatacgga cgatcgtgta ataaaccgcc gagtcaccat ggggatggta tttaccgatt
     6121 acgtcaccaa cgacacgggc agatttttta taggctttgt tccagtcatt gcctagtacg
     6181 ttcatggcgt aaagtacgcg acggtgtacc ggcttcaggc catctcggac atctggcagc
     6241 gcacggccaa caatgaccga catcgcataa tccagatagg agctctttag ctcttcctca
     6301 atgttgaccg gtgtaatttc tctcgcaagg tcgctcatct aaccgctatc cctctactgt
     6361 atcccggatt caaaggtcgc aaattataac acagccgcgc agtttgaggt aaacttatac
     6421 gctttattca catccaatgc ctgatatact cgtttgtctt gccaattacg gagtagaagt
     6481 gccaatgaat gccgaaaaat cgccggaaaa ccataacgta gaccacgaag agatcgctaa
     6541 atttgaagcc gtcgcctccc gctggtggga tcttgaaggt gagttcaaac cgttgcaccg
     6601 cattaacccg ctgcgtctgg gctatattgc cgagcgtgct ggcggtttat ttggcaaaaa
     6661 ggtgctcgat gtcggttgtg gcggcggcat tctggccgag agtatggcgc gcgaaggcgc
     6721 gacggtgacc ggtctggata tgggctttga gccattgcag gtggcaaagc tgcacgcgct
     6781 ggaaagcggc attcaggtgg attacgtgca ggaaaccgtg gaagagcacg cggcaaaaca
     6841 tgccgggcag tatgatgtgg tgacctgcat ggagatgctg gagcacgtac ccgatccgca
     6901 gtcagtggtc agagcctgtg cgcaactggt gaaaccaagc ggcgatgtct ttttctcgac
     6961 gcttaaccgc aacggcaagt catggctgat ggcggtggtt ggcgcggaat atattttgcg
     7021 catggtgccc aaaggtacgc atgatgtgaa gaagtttatt aaaccggcag agctgcttgg
     7081 ctgggtggat cagaccagtt taaaagagcg acatataacc gggctacatt acaacccgat
     7141 cactaattcc tttaagctcg gccccggcgt ggatgtgaac tatatgctgc acacgcagaa
     7201 taagtgaggt tgatgtttgg ccgcgccaat gcctgatgcg acgcttgcag cgtcttatca
     7261 ggcctacaaa tgctctccgt aggccagata aggcgtttac gccgcatccg gcaattgtga
     7321 tggtcagaca gcgattacca tttcaccgtc atcgacaaaa accctgccgt ctgggcaaaa
     7381 tcatcactcc ctttctgcca cgccacgctg ccgcgcaggg acactcgctg actgatattg
     7441 cccgtgactc ccacttttat ttcaccccgt agcttcaccg catcgtcact gatagtgctg
     7501 ccatcttctt caatttccgt cgcatgggga tcgtgataat aattcagatc taatgttggt
     7561 gtgacaccaa ccgcggtacg ccattcgctg tgtaaaccca gccgcgtctg aatatcatcc
     7621 ccctgcgatt gtgacacgcg cgcatggtta gcggcggtaa aatcatcctg ctgcacgccc
     7681 tgataaatca cctgcgcctg cggttcaatc accacaccac gccccggtaa ccactgatac
     7741 ccggcttcca gcgaggcgat aatccccgac gaatggtaac gatccacgcc atcttcatgt
     7801 tcggaaacat cattgttaaa ccacgcgtat tgcagccagc tatccagcca ggccccctgc
     7861 ttctgattac cgtgctgata ccagcttgag gtcagcccaa cggcataacc gtggttctgg
     7921 ttatcggcgc gagttccggt catattcgag cggctgtcgc cctgattatc actgtagcca
     7981 ccaaccgccc caagcatcca ctcgccatcc gtgccccagc gcccgctaaa caagccgccg
     8041 ctaagctgca ccgtagaagt gtcttcctgc tgagccagtt gccctgccgc cgtgtaatga
     8101 taacgtccgc cgataacacg taaattcagc gtctgaccat cgccaccagc gtgatcgcgt
     8161 cgctccatca taaacgccta atttgccgcc cgcaggttat tgagataacc gccaactttg
     8221 gcattcagca ccggctggta agcaggcgta ggggccgggt cgggggttgg gtcaggatcc
     8281 ggcgtgggat caggatcggg agtcgggtct ggctcaggtg gcgatggcgg cgttacttct
     8341 tgcgatcgca gacaccagtc gttgttatct tccactagcg tgtagtcata cgctcccatg
     8401 ttgacgtagc cactgccagc cagactgaac tgtgcattgt tttgaaactg cgtgggatca
     8461 gctgcgaaat caaccacttt aatgcctgtc gatgtcggct caccaatccc tgtaatgaag
     8521 ttaaccacca cagttgtgtt gccagcagta ttaccgttca tcaccaattg atcgcttacc
     8581 gagtcatcgc cgtttaattc gctgtcgaat agtagcgtac cgccgccggt ataatcaccg
     8641 tttaccgtca gcgtatcgcc agcgacgcca ttttgcaggc taaccgcacc ggacgtattg
     8701 gtcagatcac cattaaccgt cacgtcagca agataggtca ggctgtcagt ttcaaggttg
     8761 gtattaagct cgcctgtcaa cgtcacgcca tcggtagcat ttaccagcgt cccatcgccg
     8821 ttaaatagtg aggcattaag ggcgacggaa gtatcctgcc cggtcagacg caaaaccgta
     8881 ccatcgctga tgtcgatagt gccgctggaa gtagcatcaa ttgactgaat atcctgatcc
     8941 gcgccagtaa cgaacgttgc cccatcacca acccacagtg acgaagcata aggaaagata
     9001 tccgcaacat cgcctttcag ggtgccttct tcgacacgta ccgccgactg cgttgtaccg
     9061 ctggcggtca gctccagcgt acccgccccc gttttagtaa atgtgctacc ctcatcctga
     9121 tgctgcccgc tgctgtcagc catcagtgcg ccccactgcg tgtctacccc cgcatcaact
     9181 gccacttcac cgtcggcgcg catttcaata tcacgcccat gctcagtcgt cgtagagcga
     9241 gtatcgacat tggcagtaat ctccatcacg ctttcttgtt gcttatcggt aaagataacc
     9301 tggcggttat agtgggtatc tccaagctgc gagttgtcgc tcaccatcag cgtaccggag
     9361 gcgatttgtg ttgtgcccaa ataactatta ttgtttgcca gacttacctg cccgtcgctc
     9421 gcatcgacca ccacgtagac gccatctttc ccgtcgccgc cgatatgcaa attgtcgcca
     9481 cctgcaagat ctaccacatc ctgactgccg ataaccgttc cgctgctgcc actgacttca
     9541 aggccatcat tgtatgatgt gccgctctgc caggtggaga aatcagagag atcgagcaca
     9601 ccgccgttta acacgatcga ctgcggatcg tcctggagag cggtaagatc tgccgcgtcg
     9661 ccttccagcg aaagcaccgc accatcatca acgactatat cgccggttag cgccatcgac
     9721 tgcgcccctg ccagcacgta gctgccgttt tgcgcaatgg tgagctgacc agcaccttcg
     9781 atgatgccag caaaactgcc ctgattaaca gtaacgttgc caccagcatc gatatttaaa
     9841 gtgccattct gaaagcccgt caatgcgtgc acaaaagttt gttgggtcga gccaacgttt
     9901 agctcagcct gattctgata ctgatcaata ctccctatcg tcagaccgta gcagtcttgc
     9961 ggatcgtcct ggcaatgcgt atcgccgaca ttcatcaggg agttgctgcg acccagggta
    10021 acttcaccgt tttcaatctg catctcacca gtaaagtcat tgttatctgc attaagtacc
    10081 agatcgccgg aacctgtttt ggtgattaac ccggtaccag caatagagtc aacagctccg
    10141 tcattctctg tattgccgat aaccagcgtt tttccgtcgg caatatcaaa ggtaacttca
    10201 cttaagccga gatacataaa gccacccgcc gcagtggaag gaccatctcc atagcctgct
    10261 gcgctattgt tctcatcgac taacacgccg ccgttctggc tgtagctgtc atcaacagaa
    10321 atatcaataa gatagggagc cgtcgcgcta ttggtatata tcgccccgcc ataaccttcg
    10381 gctgtgttat ttgtaaaggc ggtgttattt attatcgtat aacctgaagg atgcttgctg
    10441 tcgctattat tatcggtaac atcgattgcc ccgccatcgc catcactgta acttgttgat
    10501 gtatatgctt ggttattgtt aaaaacatca tcacttaaat agacgtcatt attaatggta
    10561 taaattgcgc cacctttgcc atcattagcg atattattgc gaaacatggc gttagtgaca
    10621 cgtaaatcga cggcaccagt atcattagta ccagaagaat agattgcgcc accatagccg
    10681 cctgcgacgt taccggaaaa aataacatca gtaagattca gcgttgagtt ttctttagca
    10741 aatatggccc cgccgttatt atattctcct gtaacggtgt tattggcaaa cagagtcatt
    10801 ccagtttcat tttctggtaa tagtgaaaat tccgctcctt gttgcaaaaa tacggcccca
    10861 ccgctggcat tattggtcat atccgaaaaa accagccatt gcccatcggc
//
LOCUS       DADLSA010000126        10860 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.126, whole genome shotgun sequence.
ACCESSION   DADLSA010000126 DADLSA010000000
VERSION     DADLSA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.126"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            83..424
                     /locus_tag="JN691_003190"
     CDS             83..424
                     /locus_tag="JN691_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flxA-like family protein"
                     /protein_id="HAY7244559.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(415..1341)
                     /locus_tag="JN691_003191"
     CDS             complement(415..1341)
                     /locus_tag="JN691_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7244560.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRGAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1431..2429
                     /locus_tag="JN691_003192"
     CDS             1431..2429
                     /locus_tag="JN691_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAY7244561.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIADGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMIVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2426..2644)
                     /locus_tag="JN691_003193"
     CDS             complement(2426..2644)
                     /locus_tag="JN691_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244562.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="JN691_003194"
     CDS             complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="JN691_003194"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAY7244563.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="JN691_003195"
     CDS             complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="JN691_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAY7244564.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5948..6709
                     /gene="cysZ"
                     /locus_tag="JN691_003196"
     CDS             5948..6709
                     /gene="cysZ"
                     /locus_tag="JN691_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAY7244565.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6894..7865
                     /gene="cysK"
                     /locus_tag="JN691_003197"
     CDS             6894..7865
                     /gene="cysK"
                     /locus_tag="JN691_003197"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAY7244566.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8249..8506
                     /gene="ptsH"
                     /locus_tag="JN691_003198"
     CDS             8249..8506
                     /gene="ptsH"
                     /locus_tag="JN691_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAY7244567.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8551..10278
                     /gene="ptsI"
                     /locus_tag="JN691_003199"
     CDS             8551..10278
                     /gene="ptsI"
                     /locus_tag="JN691_003199"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAY7244568.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10319..10828
                     /gene="crr"
                     /locus_tag="JN691_003200"
     CDS             10319..10828
                     /gene="crr"
                     /locus_tag="JN691_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY7244569.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2648 a         2802 c         2684 g         2726 t
ORIGIN      
        1 tgcgatttgt aagattaatt tttcttctca agttccttgt tcttctaccg atattacctc
       61 gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc cttccataca
      121 aacacagagt agtaacaggg catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat
      181 aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag
      241 tgctgaagac aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac
      301 acaactggcg cagttgcaga aacagcaggc agaaaaggcg caggagaaag agcagcgttt
      361 gtctctgaat gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat
      421 ctgatacaac ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac
      481 gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
      541 ctggcgttca accaccggcg taatgcgctt aaccaccagt gggctacctt caggcaatgg
      601 tagcgccagt gcaggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
      661 cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc
      721 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
      781 tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
      841 gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac
      901 gatgccaaag tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
      961 atgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
     1021 aagatgcgcg gaaatggtct ggctggcagc gccccgaact tttccgctca gttgttgccc
     1081 catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa
     1141 agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
     1201 aacaccagta tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc
     1261 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
     1321 ttgttttaaa gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat
     1381 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
     1441 ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct
     1501 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc
     1561 tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct gacggtggtc
     1621 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg
     1681 tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
     1741 acttgtgcat tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
     1801 gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctgggg attttccttt
     1861 caccattgct ggttggtctg gtgatgaatg ttcacggtgc aaggggcagc cttgagcagg
     1921 tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt
     1981 ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca
     2041 ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag
     2101 ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga
     2161 ttgtagttaa cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta
     2221 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
     2281 tccccacatc ggtgatcggt atgatagtgc tgcccctgat gattttccat cagatccaat
     2341 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc
     2401 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
     2461 tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg
     2521 ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtca attaagcgac
     2581 gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
     2641 ccatctcagc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt
     2701 tcctgcgcct tcgccagttt agatcctgca gcttcacccg ctatcaccag atcggttttc
     2761 ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca
     2821 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca
     2881 atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg
     2941 ctgatgacat tgcggttgct ttcttcagca aagaagttgt gaacgtggga tgcaacgaca
     3001 atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc
     3061 gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt
     3121 gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
     3181 tgtgccaatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
     3241 agatctgccg gagtatggac atattctttt tcaaccagct gatcgatgat tttgtcgccc
     3301 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc
     3361 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg
     3421 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
     3481 tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact
     3541 ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta
     3601 ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
     3661 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat
     3721 ttaaacgcta ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc
     3781 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct
     3841 tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg
     3901 ctgaccggca acccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
     3961 tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta
     4021 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact
     4081 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
     4141 acttcaccac gcacttccag acgcgccggg atattctctc cgtgcagctt cagcggaatg
     4201 gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc
     4261 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc
     4321 agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg
     4381 aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg
     4441 cgtatctggc tgaaagctgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc
     4501 gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
     4561 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcaaagc
     4621 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
     4681 aaacccccga caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt
     4741 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
     4801 acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg
     4861 cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacttgca taaagatagt
     4921 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
     4981 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt
     5041 catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag
     5101 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt
     5161 atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
     5221 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc
     5281 gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg
     5341 ctgcaccggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg
     5401 ctggaccggt tgacgcggct gcgcagacgc ataaggcggt tggtactggt gttgcggcga
     5461 cggacgagca gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg
     5521 aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt
     5581 tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatctttctt tacggctggt
     5641 ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag
     5701 acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca
     5761 tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca
     5821 gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca
     5881 ttgcgcaatt acccggtaaa atacgcagaa ttttcctggg attggtcaaa aggagctcat
     5941 cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca
     6001 aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt
     6061 caatattttg ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat
     6121 cccgactttc atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc
     6181 tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg
     6241 gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc
     6301 tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg
     6361 cgaatggcaa aaatttgcct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt
     6421 catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat
     6481 gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat
     6541 gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct
     6601 gtttacgatg atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcaac
     6661 ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg
     6721 ttattttgta aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt
     6781 gcatgtcatt atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc
     6841 tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta
     6901 agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca
     6961 tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt
     7021 gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg
     7081 ttgaactggt tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg
     7141 ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc
     7201 tgctgaaagc gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg
     7261 caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac
     7321 aattcagcaa tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg
     7381 aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga
     7441 ctggcgtcag ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg
     7501 ttgagccaac cgattctcca gttatcgccc aggtgctggc aggtgaagag attaaacctg
     7561 gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc
     7621 tggtcgataa agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa
     7681 tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgcc gcggcgttga
     7741 aactacaaga agatgaaagc tttaccaaca agaatattgt ggttattcta ccatcatcgg
     7801 gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac
     7861 agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc
     7921 ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc
     7981 agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat
     8041 tttatgattt ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc
     8101 agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa
     8161 aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa
     8221 cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca ttaccgctcc
     8281 gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc
     8341 tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca
     8401 gactctgggc ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca
     8461 gaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt
     8521 ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt
     8581 atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa
     8641 atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca
     8701 tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa
     8761 gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata
     8821 gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt
     8881 caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta
     8941 cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc
     9001 gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag
     9061 ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc
     9121 tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct
     9181 caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat
     9241 ccaaccaacg aagttattga taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa
     9301 gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa
     9361 gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa
     9421 ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct gcccactgaa
     9481 gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc
     9541 gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa
     9601 gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg
     9661 cgcgatcagc tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc
     9721 ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa
     9781 caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg
     9841 gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt
     9901 atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt
     9961 tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat
    10021 gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt
    10081 gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc
    10141 ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag
    10201 caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa
    10261 gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat
    10321 gggtttgttc gataaactga aatctctggt ttccgacgac aaaaaggata ccggaactat
    10381 tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt
    10441 ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt
    10501 cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga
    10561 atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg
    10621 cgaaggcttc aagcgtattg ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat
    10681 tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat
    10741 ctccaacatg gacgaaatca aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga
    10801 aaccccggtt atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgcccatcgg
//
LOCUS       DADLSA010000127        10763 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.127, whole genome shotgun sequence.
ACCESSION   DADLSA010000127 DADLSA010000000
VERSION     DADLSA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.127"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            283..3150
                     /locus_tag="JN691_003201"
     CDS             283..3150
                     /locus_tag="JN691_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7244570.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3250..5178)
                     /gene="dhaR"
                     /locus_tag="JN691_003202"
     CDS             complement(3250..5178)
                     /gene="dhaR"
                     /locus_tag="JN691_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HAY7244571.1"
                     /translation="MRDMSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSA
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDAGQFKRRV"
     gene            5397..6467
                     /gene="dhaK"
                     /locus_tag="JN691_003203"
     CDS             5397..6467
                     /gene="dhaK"
                     /locus_tag="JN691_003203"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAY7244572.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYITRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6478..7110
                     /gene="dhaL"
                     /locus_tag="JN691_003204"
     CDS             6478..7110
                     /gene="dhaL"
                     /locus_tag="JN691_003204"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HAY7244573.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7121..8539
                     /gene="dhaM"
                     /locus_tag="JN691_003205"
     CDS             7121..8539
                     /gene="dhaM"
                     /locus_tag="JN691_003205"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY7244574.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            8859..10556
                     /locus_tag="JN691_003206"
     CDS             8859..10556
                     /locus_tag="JN691_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HAY7244575.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     VPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMENPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDRKKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
BASE COUNT         2814 a         2704 c         2683 g         2562 t
ORIGIN      
        1 gctgtttgtt gtaatatttt tgccatattc atagtaacga attggaataa gtaacgacaa
       61 cttagggaat aactcgatat aaactcacct ttgtaagggg ggtgttaaat atatattaat
      121 tcctgtgttc ctgattttgc ccggtaatag agaattattc tcagagaagc tcatcatgat
      181 tgcaccatac agatatcatg atgataagag gatattattc attgacttaa acaaaatgac
      241 aaaatccata gacccatagc ctgggtacta tataggagtg gtatgggcat caaacaacac
      301 aatgggaata ccaaagccga tcgtctcgct gaattaaaaa tccgttcgcc ctcaattcaa
      361 ctgataaaat ttggcgctat tggtttgaat gcaattatct tttcccccct gctgatagct
      421 gctgatacag gaagtcaata tggcaccaat attactatta atgatggtga cagaattaca
      481 ggagataccg ccgatccatc aggaaacctc tatggtgtaa tgaccccagc aggaaacacg
      541 cctggcaata tcaacctggg taatgatgtc accgtcaatg tcaacgacgc ctctggatat
      601 gcaaaaggaa tcattattca gggcaaaaac agctccctga cagctaaccg actcacagta
      661 gatgttgttg gtcaaacctc tgccatcggc attaacttaa ttggtgacta tacccatgct
      721 gacttaggca caggcagcac cattaagagt aacgatgacg gcatcattat tgggcatagc
      781 tcaacactaa cagccactca attcaccatt gaaaactcga acggtatagg cctaaccatc
      841 aatgactatg gcaccagtgt cgatcttgga agcggaagta aaatcaagac cgatggaagt
      901 acaggtgttt atatcggtgg tctcaacggc aataacgcca atggtgctgc gcgttttacg
      961 gcgacagacc tgacaatcga tgttcagggc tacagcgcca tggggataaa cgtacagaaa
     1021 aactctgttg tcgatctcgg aacaaacagt accattaaaa ccaatggcga taatgctcac
     1081 ggcctctgga gctttggcca ggttagcgcg aatgcactca ctgttgatgt aactggagcc
     1141 gcggccaatg gcgtcgaagt tcgtggtggt acaaccacta tcggtgcaga tagccatatt
     1201 tcttccgcgc agggcggtgg cctcgtcacc agtggttcag acgcgacaat caatttttct
     1261 ggcacggcag cgcaacgaaa cagcatcttt tccggcggtt cttatggtgc ctcggcccag
     1321 acggcaacgg ctgttatcaa catgcaaaat accgatatta cggttgatcg taatggcagt
     1381 ctggcgctgg gtttgtgggc gctcagcggc ggtagaataa ccggagacag tttggctatc
     1441 accggcgcgg caggagccag aggtatttat gccatgacca acagccagat cgacctcacg
     1501 agcgatctgg tcattgatat gagtacaccc gaccagatgg ccatcgcaac gcaacatgac
     1561 gatggttatg ccgccagccg catcaacgcc tcgggtcgta tgcttatcaa cggtagcgtt
     1621 ctttccaaag gtgggctaat caatctggat atgcaccctg ggtcggtttg gacaggttcc
     1681 tccctcagcg ataatgtcaa tggcgggaaa ctggacgttg caatgaataa cagcgtctgg
     1741 aacgtaacaa gtaattctaa tctcgacacg ctggcgctga gccattcaac tgtcgatttt
     1801 gccagccacg ggtcaactgc cggcacattt accacattaa acgtagagaa cctgagcggt
     1861 aacagtacct ttattatgcg tgctgatgtt gttggcgagg gtaatggcgt taataataga
     1921 ggggatttat tgaatatcag cgggagtagt gctggtaatc acgtattggc tatccgcaac
     1981 cagggcagcg aggccacaac gggaaatgaa gttctgacag tggtaaaaac cactgacggc
     2041 gcggcctcgt tcagcgcgtc ttctcaggtt gagttggggg gatatctgta cgatgtgcgt
     2101 aaaaatggca ctaactggga gctttacgct tccgggacag ttccggaacc gactcctaat
     2161 cctgaaccca caccagctcc cgctcagcct cccatagtca accccgatcc tacgcctgaa
     2221 cccgctccca cgcctaaacc caccacgacc gcagatgctg gcggcaatta tctcaatgtc
     2281 ggttacttat tgaactatgt tgaaaaccgt acgctgatgc aacggatggg tgacttgcga
     2341 aatcagagta aagacggtaa tatctggttg cgcagttatg ggggaagctt ggactccttt
     2401 gccagtggca aactgagcgg ctttgacatg ggttacagcg gtatccagtt tggtggggat
     2461 aaacgtctct ctgatgtaat gccgttgtat gtcggtctgt atattggctc aacacatgca
     2521 tcgccggact atagcggagg cgacggtacc gcacgttcag actacatggg aatgtacgcc
     2581 agttacatgg cacaaaacgg tttttacagc gatctcgtta taaaagcatc gcgccagaaa
     2641 aatagtttcc acgtactgga cagtcagaac aacggcgtta acgccaacgg cactgcgaat
     2701 ggaatgagca tctccctgga agccgggcag aggttcaacc tgtcccctac tggttatggg
     2761 ttctatatag agccgcaaac ccagcttaca tacagccacc agaatgagat ggctatgaag
     2821 gcgagtaatg gcctcaatat acatctgaat cactacgaat cgctgctggg gcgtgccagc
     2881 atgatactgg ggtatgacat caccgcaggc aacagccagc tgaatgtcta tgtgaagact
     2941 ggcgctatcc gcgagttttc aggggatacc gaatatctgt tgaacgactc ccgggagaag
     3001 tacagtttca aaggtaatgg ctggaataac ggcgtgggag tcagtgcaca gtataacaaa
     3061 cagcacacat tctatctcga agcggattac acgcagggta acctctttga tcagaagcaa
     3121 gtcaacggag gatatcgctt cagcttttaa gtgcgggata gacaagccct taaacgatgc
     3181 tgaaaattgt tagcgtgcgc cggaatgaaa tacagatcac tctgatgtat cagaaatcgt
     3241 ttctgtcttt catacccggc gcttaaactg ccctgcatca atgccatgtt gcttcatttt
     3301 ccgccacagc gtagtgcggc cgatcccaag taaagccgac atttcctgaa tgcgaccgcc
     3361 tgtgacttgg gctgcgttaa taattgcctc tttttcaact tccgcaaatg acagactggt
     3421 ggaaaggcgg gtggcgctga catcatctgt cgcctgctcg gtaaacagat gttcaggtaa
     3481 atcactgacg cgaatgcgcc cgttatcact actcagagca agattctcga tgacgctgta
     3541 aagttcaaaa tcgttgcctg gccatgcaca agaaaccagg cgagcgaggg catcgtcatc
     3601 aattttcagc cgcgtagaga agcgtttttc aagactgcgt aatttgttat tcaccagcgc
     3661 cggaatgctg ccacgccgca tacgcagagg cgggatggta atttcaaatg catgcagcgc
     3721 gtaatacagc tggcgactaa aacgattttg ttccaccagc attgcgaggt ccgcggtcgt
     3781 tgttgcaatc actttgacat caattggtat taaacgccgc gcatccagtc gcgtgataac
     3841 cccctgcttg ataacctgaa gcaaagcaga ctgtaactcc accgccagat attcaatctt
     3901 ttcaagaaac agcgtgccgc cgtgtgccag ttccagccga ctcagacggc cattttcatt
     3961 gtccgtgcga tcgccaccaa taaattcttc cgccagcgca gcatcaccat ataactcaca
     4021 attgacggcg atataaggac ctgcagcacg ctcgctttca ttatgaattg cctgacttag
     4081 cagtgccttg cccacgccct cttctccaca aagcaggaca ggaaagctac tgcgcgccgc
     4141 ctggcgacca aaatgaatca agcggcgggt ttgcggatcg tcctgtggca tatgagcgaa
     4201 ggtatggctg acttttccta attgactggt cattaactgc cgcatctgtt ccacaggatg
     4261 gagcaacaaa ataaagctgg ttccctgcgt ttctattatc ggtttaaggg ttatcaccgc
     4321 atcaataaac tggtgctggc tttcaaaggt tgcttctacg tgtttgagcg gatgtgcctg
     4381 ttttattgct tgttgcaata cggcgggtaa cgttaagagt tcagtgattg cccgtccctg
     4441 acttgccgtc gcgtcaaggc gcaagacccg cgccgcctgg gcattaataa attgcaaatt
     4501 accctgctcg tcccagctaa tcacgccatc atccatactt tctaacaggg cattaagttg
     4561 atttaaatga cggttagttt cagcgagcaa actgtccgtc agcagtaaat taccgacctc
     4621 gcgggcgatt gccaacgtca acggcaaatc agctgcggta gtttgctcaa ccggacacgc
     4681 cagcgctatt gttcccgtca atcggccctt gctgtcaaac aacggcgttg cacaaaaggc
     4741 ccagttccag agtgcctgtt tgaaatgttg atcggccatc gttttcacgg cctgaccaga
     4801 gatagccgct aacgatagcg cacaagtacc aataattccc tcggcgcaat acgtgccgtc
     4861 attgaatccc agtgcactta gctgctgcaa ggtttgcgga tcgccattac ggctgagaat
     4921 gcaggcggtt tcatcgagga taaacagcgc acactctcgc ggtgccatat attcccaggc
     4981 atcttccagc gcagcctgcc cgagcgtcag catcgctttc ttacgccgat aaatagaagc
     5041 aaatgtcacg ccctgggcct gatgtggtac gttccatgtc tcccgtttca tcagcttatt
     5101 gcatcgctcc caggaggttg caattaaggg agatatgccc cgaccatcgt tgttaaaagc
     5161 gccactcata tcccgcatcc cttatgacaa aaggtgttcc ggctcgcaat tttaagtatt
     5221 aatacacgta aaagtttcaa tatggaacat tcattaaagc atatgtttca ctatgaaaca
     5281 taatttgggc taattttctt ttcccttgcc tgatgcacaa tggattcatc ggcagtcagt
     5341 ggtcgccgtg tcgttgaaca tcatccatgc cctaccgtaa ttgctggagc aaaataatga
     5401 aaaaattgat caatgatgtg caagacgtac tggacgaaca actggcagga ctggcgaaag
     5461 cgcatccatc gctgacactg catcaggatc cggtgtatat cacccgagct gatgcccctg
     5521 ttgcaggaaa agtcgccctg ctgtcgggtg gcggcagcgg acacgagccg atgcactgtg
     5581 gttatatcgg tcaggggatg ctttcggggg cctgtccggg cgaaattttc acctcaccga
     5641 cgcccgataa aatctttgaa tgctccatgc aaattgatgg cggcgaaggt gtactgttga
     5701 ttatcaaaaa ttacaccggc gatattctta actttgaaac agcgaccgag ttactgcacg
     5761 atagcggcgt aaaagtgacc actgtggtca ttgatgacga cgttgcggta aaagacagtc
     5821 tttataccgc cgggcgacgc ggcgttgcca acaccgtatt aattgaaaaa ctcgtaggcg
     5881 cagcggcaga gcgtggcgac tcactggacg cctgtgcgga actggggcgt aagttgaata
     5941 atcaaggcca ctcaataggt atcgctctcg gtgcctgtac cgttcctgcc gcgggcaaac
     6001 cttcttttac ccttgcggat aatgagatgg agtttggcgt cggcattcat ggtgagccag
     6061 gtattgaccg ccgccccttc tcttcccttg atcaaaccgt cgatgaaatg ttcgacaccc
     6121 tgctggaaca tggctcatac catcgcactt tgcgtttctg ggattatcaa caaggcagct
     6181 ggcaggaaga accacaaacc aaacaaccgc tccagtctgg cgatcgggtg attgcactgg
     6241 ttaacaatct tggcgcaact ccgctttctg agctgtacgg cgtctataac cgcctgacca
     6301 cacgttgcca gcaagcggga ttgactatcg aacgtaattt aattggcgcg tactgcacct
     6361 cactggatat gaccggtttc tcaatcacct tactgaaagt tgatgacgaa acgctggctc
     6421 tctgggacgc cccggtccac accccggccc ttaactgggg taaataagga gaaagcaatg
     6481 tcactgagca gaactcaaat tgttaactgg ctcactcgtt gtggcgatat tttcaacacc
     6541 gagagcgagt atcttaccgg gctggatcgc gagattggcg acgctgacca cgggctaaat
     6601 atgaaccgag gctttagcaa agtggtggaa aaactccctg ctatcgcaga taaagatatc
     6661 ggtttcattc tcaaaaatac cggtatgacg ctgctttcca gcgtcggtgg tgccagtggt
     6721 ccgctgttcg gtaccttctt tatccgcgcc gcacaggcga cccaggcacg gcaaagcctg
     6781 acactggaag agctttatca gatgttccgc gatggcgcgg acggcgtaat cagtcgcggg
     6841 aaagccgaac ctggcgataa aaccatgtgt gatgtgtggg tgccggtggt ggaatcgtta
     6901 cgtcagtcca gcgagcaaaa tctctctgtt ccggtggcgc tcgaagctgc cagtagcatc
     6961 gccgaatccg ctgcacaaag tacgattacg atgcaagccc gcaaaggccg cgccagttat
     7021 ctcggtgaac gcagtattgg tcaccaggat cccggcgcga cctcggtgat gtttatgatg
     7081 caaatgttgg cgttagccgc aaaagagtaa ggaattggtg atggtaaacc tggtcatagt
     7141 ttcacatagc agccgactgg gagaaggtgt cggtgaatta gcccgtcaga tgttaatgag
     7201 tgatagttgt aaaatcgcca ttgccgcggg aattgacgat ccacaaaatc ccattggtac
     7261 cgatgccgtc aaagtgatgg aggccatcga atctgttgct gatgccgacc atgtgctggt
     7321 catgatggat atgggtagcg cattattgag tgctgaaact gcgctggaat tgctggctcc
     7381 cgagatcgcc gcaaaagtac gtttgtgtgc tgcgccgttg gtcgaaggta cactggcagc
     7441 aacggtcagc gcggcctcgg gggcggatat cgacaaagtt atctttgacg ctatgcatgc
     7501 gctggaagcc aaacgtgaac aactgggttt accgtcctcc gacactgaaa tctctgacac
     7561 atgtcctccg tacgatgaag aagcccgttc tctggcggtg gtcataaaaa accgtaacgg
     7621 cctgcatgta cgtccggcct cccggctggt ttatacctta tcgacattta atgccgatat
     7681 gttgctggaa aaaaacggca aatgcgtcac accagagagt attaaccaga ttgcgttact
     7741 acaagttcgc tataacgata cgctgcgcct gattgcgaaa gggccagaag ctgaagaggc
     7801 actgatcgct ttccgtcagc tggctgaaga taactttggt gaaacggagg aagtcgctcc
     7861 acctactctg cgtcccgttc tgcctgtttc gggtaaagcc ttttattatc aaccagtttt
     7921 atgtacggta caggcaaaat caaccctgac cgcggaagaa gaacaagatc gattacgcca
     7981 ggctattgac ttcacgttat tagatctgat gacgttaaca gcgaaagcag aagccagcgg
     8041 gcttgacgat attgccgcaa tcttttctgg tcaccataca ctgttagatg atccggaact
     8101 gctggcggcg gcaagcgaac tccttcagca tgaacattgc acggcagaat atgcctggca
     8161 gcaagttctt aaagaactta gccagcaata ccagcaactg gatgatgaat atctacaagc
     8221 tcgctatatt gatgtggacg atcttctgca tcgcaccctg gtccacctga cccaaacgaa
     8281 agaagaactc ccgcagttta actcgccaac tattctactg gcggagaaca tttatccttc
     8341 cacagtactg caactggatc cggcggttgt aaaaggtatc tgccttagcg ccggaagtcc
     8401 ggtatcccac agcgccctaa tcgcccgtga actggggatt ggctggattt gccagcaggg
     8461 tgagaaactg tatgcgatac aaccagaaga aacgctaacg ctggacgtta aaacgcaacg
     8521 tttcaaccgt cagggttaag ccaccgttcg gatggcatcg ttctgatgtc atccggcaat
     8581 ttacccagca ctgatgtttt ttaactacct gttatatttt ttaccaattt atgatgattt
     8641 ttcttcgcta atcgcgaacc aaatgtcgct ggctggctaa taaacagacg ttatttgatc
     8701 actctatttc gccgttattt atccctttaa ttcccttttc taaaatgcct gaccgttcgc
     8761 agaatgagat ttcgatcatg cagctagtgc gatcctgaac taaggttttc tgatacttga
     8821 ataccgtttt attcggtttc gccaaaggag aatgattgat gaaatccccc gcaccttctc
     8881 gcccgcaaaa aatggcgtta attccagcct gtatcttttt gtgtttcgct gcgctatcgg
     8941 tgcaggcaga agaaacaccg gtaacaccac agccgcctga tattttatta gtgccgctgt
     9001 ttaatgatgt gcaaaacgcc aaactttttc cggaccaaaa aacctttgcc gatgccgtgc
     9061 cgaacagcga tccgctgatg atccttgctg attatcggat gcagcaaaac cagagcggat
     9121 ttgatctgcg ccatttcgtt aacgtcaatt tcaccctgcc gaaagaaggc gagaaatatg
     9181 ttccgccaga ggggcagtca ctgcgcgaac atattgacgg actttggccg gtattaacgc
     9241 gttctaccga aaacaccgaa aaatgggatt ctctgttacc gctgccggaa ccttatgtcg
     9301 tgccgggcgg acgttttcgc gaggtatatt actgggacag ttacttcacc atgttaggac
     9361 ttgccgaaag cggtcactgg gataaagtcg cggatatggt ggccaatttt gctcatgaaa
     9421 tagacactta cggtcatatt cccaacggca accgcagtta ctatttaagc cgctcgcaac
     9481 cgcccttctt tgccctgatg gtagagttac tggcgcagca tgaaggcgat gccgcgttga
     9541 agcaatacct gccgcaaatg caaaaagaat atgcttactg gatggacggt gttgaaaacc
     9601 tgcaagccgg acaacaggaa aaacgcgttg taaaacttca ggatggtacc cttctcaacc
     9661 gctactggga cgatcgcgat acgccacgac cagagtcatg ggtggaagat attgccaccg
     9721 ccaaaagcaa tccgaatcga cctgccactg aaatttaccg cgacctgcgc tctgccgctg
     9781 cgtctggctg ggatttcagc tcgcgctgga tggagaaccc gcagcagtta aataccttac
     9841 gcaccaccag catcgtaccg gtcgatctga acagcctgat gtttaaaatg gaaaaaatcc
     9901 tcgcccgcgc cagcaaagct gcgggagata acgcgatggc aaaccagtac gaaacgctgg
     9961 cgaatgcccg tcaaaaaggg atcgaaaaat acctgtggaa cgatcaacaa ggctggtatg
    10021 ccgattacga cctgaaaagt cataaagtgc gcaatcagtt aaccgcggcc gccctgttcc
    10081 cgctgtacgt caatgcggca gcgaaagatc gcgccaacaa aatggcgacg gcgacgaaaa
    10141 cacatctgct gcaacccggc ggcctgaaca ccacgtcggt gaaaagtggg caacaatggg
    10201 atgcgccaaa tggctgggca ccgttacagt gggtcgcgac agaaggatta caaaactacg
    10261 ggcaaaaaga ggtggcgatg gacattagct ggcacttcct gaccaatgtt cagcacacct
    10321 atgaccggaa gaaaaagctg gtggaaaaat atgatgtcag caccaccgga acggggggcg
    10381 gcggtggcga atatccatta caggatggct ttggctggac caatggcgtg acgctgaaaa
    10441 tgctggattt gatctgcccg aaagagcaac cgtgtgacaa tgttccggcg acgcgtccga
    10501 ccgttaagtc agcaacgacg caaccctcaa ccaaagaggc acaacccaca ccttaaccag
    10561 cacttactcc gacaacgtca ttcggcttct ccggatggcg ttgtcggcag ataacaccat
    10621 atctatggtt ttttctacac agacggtttt tcaagtcctg gcgggaagta ttgcgcaaat
    10681 gtttgccata cgtggttgcc gaagatagcg tcatttctac gggtaagtgt ttcactttca
    10741 tcacccggcg atttacgccg acg
//
LOCUS       DADLSA010000128        10712 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.128, whole genome shotgun sequence.
ACCESSION   DADLSA010000128 DADLSA010000000
VERSION     DADLSA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.128"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            429..1547
                     /locus_tag="JN691_003207"
     CDS             429..1547
                     /locus_tag="JN691_003207"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY7244576.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWNIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1613..1861
                     /locus_tag="JN691_003208"
     CDS             1613..1861
                     /locus_tag="JN691_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HAY7244577.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1926..2294
                     /locus_tag="JN691_003209"
     CDS             1926..2294
                     /locus_tag="JN691_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY7244578.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKPDQQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2388..3041
                     /gene="nfsB"
                     /locus_tag="JN691_003210"
     CDS             2388..3041
                     /gene="nfsB"
                     /locus_tag="JN691_003210"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAY7244579.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDATILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3149..4396
                     /locus_tag="JN691_003211"
     CDS             3149..4396
                     /locus_tag="JN691_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAY7244580.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKDTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="JN691_003212"
     CDS             complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="JN691_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HAY7244581.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCISFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5941..9084)
                     /gene="cusA"
                     /locus_tag="JN691_003213"
     CDS             complement(5941..9084)
                     /gene="cusA"
                     /locus_tag="JN691_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HAY7244582.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9096..10319)
                     /gene="cusB"
                     /locus_tag="JN691_003214"
     CDS             complement(9096..10319)
                     /gene="cusB"
                     /locus_tag="JN691_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HAY7244583.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFEQAEPHAEKMPTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRHLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPNKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSGGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10335..10667)
                     /gene="cusF"
                     /locus_tag="JN691_003215"
     CDS             complement(10335..10667)
                     /gene="cusF"
                     /locus_tag="JN691_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HAY7244584.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
BASE COUNT         2595 a         2959 c         2607 g         2551 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagggttg atagaaatat
       61 ctcctggggc ttcatctttc cagacctcag agtaaacctg aaatcggaaa gcttcaggca
      121 cccgcagtta tcttaccctt cctcaacaaa atagctgttt atttatacag caaatcctgt
      181 ttcggacttc tcactcccca tttgatcaac tgaagctgaa cccgtttttt tctgcatgcc
      241 tcaatcgtca aaattcttcg ccaccagcca gctctgccat aacttgccac attgaaaaag
      301 tagccaaagt gcttcaggca ccatcagttc accatcacca aaaaaaagaa acattccaga
      361 acttccgcat ctcgcccagc cgctccgact tctatactga atagaaaacg tcaacttaag
      421 agaaacctat gccattaccc gattttcatg tttctgaacc ttttaccctc ggtattgaac
      481 tggaaatgca ggtggttaat ccgccgggct atgacttaag ccaggactct tcaatgctga
      541 ttgacgcggt taaaaataag atcacggccg gagaggtaaa gcacgatatc accgaaagta
      601 tgctggagct ggcgacggat gtttgccgtg atatcaacca ggctgccggg cagttttcag
      661 cgatgcagaa agtcgtattg caggcagccg cagaccatca tctggaaatt tgcggcggtg
      721 gcacgcaccc gtttcagaaa tggcagcgtc aggaggtatg cgacaacgaa cgctatcaac
      781 gaacgctgga aaactttggc tatctcatcc agcaggcgac cgtttttggt cagcatgtcc
      841 atgttggctg tgccagtggc gatgacgcca tttatttgct gcacggcttg tcacggtttg
      901 tgccgcactt tatcgccctt tccgccgcgt cgccatatat gcagggaacg gatacgcgtt
      961 ttgcctcctc acgaccgaat attttttccg cctttcctga taatggcccg atgccgtggg
     1021 tcagtaactg gcaacaattt gaagccctgt ttcgctgtct gagttacacc acgatgatcg
     1081 acagcattaa agatctgcac tggaatattc gccccagtcc tcattttggc acggtggagg
     1141 ttcgggtgat ggataccccg ttaaccctta gccacgcggt aaatatggcg ggattaattc
     1201 aggccaccgc ccactggtta ctgacggaac gcccgttcaa acatcaggag aaagattacc
     1261 tgctgtataa attcaaccgt ttccaggcct gtcgctatgg tctggaaggc gtcattaccg
     1321 atccgcacac tggcgatcgt cgaccgctaa cggaagatac cttgcgattg ctggaaaaaa
     1381 tcgccccttc agcacataaa attggcgcat cgagcgcaat tgaagcactg catcgccagg
     1441 tcgtcagcgg tctgaatgaa gcgcagctga tgcgcgattt cgtcgccgat ggcggctcgc
     1501 tgattgggct ggtgaaaaag cattgtgaga tctgggccgg tgactaagcc gggaattgcc
     1561 ttacagaaga atatccctga caatggtgtt ttaacttaca tttaacaatg ttatgaaaca
     1621 ccctttagaa accttgacca ccgcagcagg cattttgctg atggctttcc tctcttgcct
     1681 gctgctgccc gcccccgcac tggggctgac gctggcacaa aaactggtga ccatgttcca
     1741 tctgatggat cttagccagc tttacacttt attgttttgt ctgtggtttt tagtgctggg
     1801 cgctattgag tattttgttc tgcgctttat ctggcgacgc tggttctcgc tggcggatta
     1861 aacgtcgatt atcgccgaac ttcgcggcat actttgctta ttctccttcg cctaaaggaa
     1921 cgtttatgga taagcaatca ctgcacgaaa cggcgaaacg cctagctctt gagttaccct
     1981 ttgtcgagct ttgctggcct tttggcccgg agttcgatgt ttttaaaatt ggcggcaaga
     2041 tttttatgct gtcgtcggag ctacgcggcg tcccctttat caatctgaag cccgatcaac
     2101 aaaaatccct gttaaatcag caaatatacc caagcattaa gccagggtat cacatgaata
     2161 aaaagcactg gatttcggtg tattccggcg aggaaatctc cgaagcgtta cttcgcgatc
     2221 tgatcaacga ttcgtggaat ctggtggttg atggtctggc taaacgcgat caaaaaagag
     2281 tgcgtccagg ctaaagcggg aatctatagc gcatttttct cgcttaccat ttctcgttga
     2341 accttgtaat ctgctggcac gcaaaattac ttacacatgg agtctttatg gatatcattt
     2401 ctgtcgcctt aaagcgtcat tccactaagg catttgatgc cagcaaaaaa cttaccccgg
     2461 aacaggccga gcagatcaaa acgctactgc aatacagccc atccagcacc aactcccagc
     2521 cgtggcattt tattgttgcc agcacggaag aaggtaaagc gcgtgttgcc aaatccgctg
     2581 ccggtaatta tgtgttcaac aaacgtaaaa tacttgatgc ctcgcacgtc gtggtgttct
     2641 gtgcaaaaac cgcgatggac gatgcctggc tgaagctggt tgttgaccag gaagatgccg
     2701 atggccgctt tgccacgccg gaagcgaaag ccgcgaacga taaaggtcgc aagttcttcg
     2761 ccgatatgca ccgtaaagat ctgcatgatg atgcagagtg gatggcaaaa caggtttatc
     2821 tcaacgtcgg taacttcctg ctcggcgtgg cggcgctggg tctggacgcg gtgcccatcg
     2881 aaggttttga cgccacaatc ctcgatgcag aatttggtct gaaagagaaa ggctacacca
     2941 gtctggtggt tgttccggta ggtcatcaca gcgttgaaga ttttaacgct acgctgccga
     3001 aatctcgtct gccgcaaaac accaccttaa ccgaagtgta attctctctt gccgggcatc
     3061 tgcccggcta tttcccctca gattctcctg atttgcataa ccctgtttca gccgtcatca
     3121 taggctgctg ttgtataaag gagacgttat gcaggattta atatcccagg ttgaagattt
     3181 agcgggtatt gagatcgatc acaccacctc gatggtgatg attttcggta ttatttttct
     3241 gaccgccgtc gtggtgcata ttattttgca ttgggtggta ctgcggacct tcgaaaaacg
     3301 tgccatcgcc agttcacggc tttggttgca aatcattacc cagaataaac tcttccaccg
     3361 tttagctttt accctgcagg ggattatcgt caatattcag gcggtattct ggctgcaaaa
     3421 agacaccgaa gcggcagata ttctgactac ctgcgcgcag ttgtggatca tgatgtatgc
     3481 gctgctttca gtcttctcgt tgctggatgt tattttgaat ctggcgcaga aattcccagc
     3541 ggcatctcag ttaccgctga aagggatatt tcaggggatt aaactgatcg gcgcaatcct
     3601 ggtcggcatt ttgatgatct cgctgctgat tggtcagtcg ccagcgattc tgatcagcgg
     3661 tcttggtgca atggctgccg tgctgatgtt ggtatttaaa gatccgattc ttggtctggt
     3721 ggcaggtatt cagctttccg cgaacgatat gctgaaactg ggcgactggc tggaaatgcc
     3781 gaaatacggc gcggatggcg cggtgatcga tattgggtta accaccgtca aagtgcgtaa
     3841 ctgggacaat accattacca ctattcccac ctggtctctg gtttctgact cctttaaaaa
     3901 ctggagcggg atgtcagcat ctggcgggcg acgtattaag cgcagtatca gtattgatgt
     3961 caccagtatt cgttttcttg atgaagacga aatgcaacgt ctgaataaag cgcatttgtt
     4021 aaagccttat ttaaccagcc gccatcagga aattaatgag tggaatcgcc agcaaggttc
     4081 tacggagtcg gtattaaacc tgcgccgaat gaccaatatt ggaacctttc gtgcctatct
     4141 gaacgaatat ctgcgtaacc atccgcggat tcgtaaagat atgaccttaa tggtacgcca
     4201 actggctcca ggtgataacg gtttaccgct cgagatctac gcgtttacta acaccgtggt
     4261 gtggctggaa tatgaaagca ttcaggctga tatattcgat cacatatttg cgattgtcga
     4321 agagtttggt ctgcgacttc atcagtcgcc aaccggcaat gatattcgct ctctggcggg
     4381 tgcatttaag cagtaattaa aaaaaccgct ctcatcgaat ggatgagagc ggacatcgtt
     4441 ttgtcggatg tagcgtgaat gccttatttc cgacgcagcg ttttaaatgc cacaaacagg
     4501 aatacaatcc acaccggcag caggatcgct gacaagcgca tatcatccat cgtgcacatc
     4561 agcagcaaaa tcatgccgag gaaggagatg caaagatagt tgccgaacgg atagagcagc
     4621 gccttaaact gtgtttcacg cccctgacgt cgcatcgccg cgcgaaaacg cagatgcgcc
     4681 aggcagatca taatccagtt caacagcaac gttgctacca ccagcgccat cagcagacca
     4741 aacgcttttt gcggcagcag atagttgatt aacaccacca gcgaagtgat cgctccggaa
     4801 agcatcagcg agttaatcgg cacaccgcga cggctgacgc gagtcaaaaa cttcggcgca
     4861 ttaccctgca cagaaaggcc aaacagcatg cggctgttag agtaaacccc gctgttatac
     4921 accgacagcg atgccaccag aatgacgaag ttcagcgcag aagctaccac attgctgtcg
     4981 agattatgga aaatcatcac aaacgggcta ctgttggatt tcacttccac ccacggatag
     5041 agtgccagta aaaccaccag tgaaccgata taaaacagca ggatgcgata caccacctga
     5101 tttaccgctt ttggaatgct tttttccgga tcgcgcgctt cagcggctgt aatcccaatc
     5161 agctccagac cgccgaagga gaacataatt accgccagcg acaaaatcag cccattccag
     5221 ccggtggcga agaaaccacc gtagcgccag aggttgtcga tactggcttt ctcgccgccg
     5281 tgaccagaaa acagcagcca caggccaaag ccgatcatac cgatgatcgc cagcacttta
     5341 atcagcgcaa accagaactc ggtttcgcca tataagcgca cgttcaccag gttaacggcg
     5401 ttgatgataa taaagaaggc ggcagcccaa atccacgttg gaacatccgg gaaccagtac
     5461 tgcatataga tgcccgcagc ggtcagctct gccattccca ccagcacgaa cattacccag
     5521 tagttccagc cagagaggaa gcccgcaaac ggtccccagt atttataggc aaagtgggca
     5581 aatgaaccgg ataccggctc ttcgaccacc atctcgccaa gctggcgcat aatcaggaaa
     5641 gcgatgatcc cggcgacgcc gtagcccagc aatacagccg gacccgccat ctgaatcgcc
     5701 gggccaatgc caagaaacag accagtacca attgcgccac ccagcgcaat cagttgaata
     5761 tgacggttat gtaatccgcg atgaagcgtc ggctcttgat tcgacgcagt atcttccgat
     5821 acggttgacg cgtttttcac gcctttcccc tgtgtgtctt ttttgttgag gggcaccttt
     5881 taacatttag tgcccatcgt cgcaagacac aatccacacg gttaaacgag gtatcctgct
     5941 ttaattccgt acccgatgtc ggtgcagcca catcagctta tacgccgccg ggataataaa
     6001 cagcgacagc aaaggtgcgg tgatcatgcc gccaatcatc ggtgcggcaa tccggctcat
     6061 cacctctgaa ccagcccccg ttccccacag aatcggcagc agaccggcga taatcaccgc
     6121 caccgtcatc gctttcgggc gcacgcgcag gaccgcgccg tgatacaacg cctcatccag
     6181 cttctgctcg ctgaatgttt gtggattatt caacgacggc tcggcctcta tggcgtgacg
     6241 taaatacatc agcatcacca cgccaaattc ggcggcgacc ccggcaaggg caataaagcc
     6301 ggtacccgtc gccacagaaa gatgaaagcc catccaccac agtaaccaga tgccgcccac
     6361 cagcgcaaac ggtacgctgc tgataatcag caacgcttcg cccacccgac ggaatgccag
     6421 atacaacagc acgaagatga tcatcagtgt catcggcacc atgagtttca gcttatggtt
     6481 ggcgcgctcc agtagctcga actgcccgga gaatgccacg ctggtgcccg gttttaactg
     6541 cactttttca gctatcgctt tttgcaaatc gtgaaccacc gacaccatgt cacgatcgcg
     6601 ggcatcgata taaatccagc tcgtcgggcg cgcattctcg gttttcagca tcgacggtcc
     6661 ggtagagact ttaacgtcgg ccacgtctgc cagggtgatt tgctgcttca tcggcgtcag
     6721 gatcggcaac tggcgcagtg cctgcggact atcgcgccag ctttgcggat aacgcagatt
     6781 aattggataa cgggcaatcc cttccaccgt ttcgccaacc atcgccccgc ccaccgcaga
     6841 agtcacaaac aactgcacat ccgccaccgt cataccgtaa cgtgcggctt tttcacggtt
     6901 aatctcaacg ttgatatagc gcccaccttc cagccgctcg gcaagcgcag aagctacgcc
     6961 tggcaccgtt cgcgccactt cttcaatttg ctcagccatc gcgtcgatat ccgccagcac
     7021 agtgccggaa actttaatgc cgatggggct tttaatgccg gttgagagca tatcgatacg
     7081 gttacgaatt ggcggcaccc acagattcgc cagccccggc agacgcacgg tgttatccag
     7141 ttcctcaatg attttgtcca ttgtcatgcc tggccgccac tgatcctgtg gcttaagctg
     7201 gatggtcgtt tctaccatct ccagcggagc agaatcggtg gcggtttccg ctttcccggt
     7261 tttgccaaat acccgcgcca cttcaggtac gctcataatt agcttgtcgg ttttttgcag
     7321 catactcgcc gcctctgctg cggaaatccc cggcagcgtc gatggcatat acaacaagtc
     7381 gccttcattg atctgcggta aaaattcccc gccaacttta ttgagcggcc agagaaccgt
     7441 cagcaccgaa agcgccgcca ccagcagcgt ggttttcggc cagtgcagta ctttcagcaa
     7501 cagcggatga taaacacgaa tcaaaaagcg attgagcgga ttactgcttt ccggcggaat
     7561 tttgccacgg atccagtaac ccatcaggat agggatcact acgatcgcca gcagcgccgc
     7621 acccgccatc gcatacgttt tggtgaacgc caacgggcca aacagacgcc cttcctgccc
     7681 ttccagggtg aagatcggga taaacgacaa cgtgataatc agcagactga taaacagcgc
     7741 cggccccact tcaacagacg catcggtgat cacctgccag cgcgttttat tatccagcgt
     7801 ggcgtcagga tgctggtgct gccactcttc cagccgttta tgcgcattct cgatcatgac
     7861 gatagcggca tcgaccatcg ccccgacggc aatcgcaatg ccacccagcg acataatatt
     7921 ggcattcagc ccctggaagg gcatgacaat aaaagcaata cacaacccca gcggcaacga
     7981 aataatcgcc accagcgccg agcgcacatg ccagagaaac agcgcgcaga ctaccgccac
     8041 cacaataaac tcttccagca acttgccgct gaggttgtcg atggcccggt caatgagctg
     8101 gctgcggtcg tatgttgtaa ctatctccac gccttccggc agactacttt tcagcgtttc
     8161 cagtttgtcc ttcacggcgg cgatcacttc tcgggcgttt ttgccggatc gcaggatcac
     8221 caccccgccc gccacttcgc cttcgccgtt tagttcggca atgccccggc gcatctccgg
     8281 gccaatctgg accttcgcaa catcgcgcag ataaacgggc acgccatttt cactggcttt
     8341 taaaacgatg tgattaaagt cgtcgagcgt ttgtagatag ccgctggcgc gcaccatata
     8401 ttctgcttcc gccagttcga ttgacgaacc gcccgcttcc tggtttgaag catccagcgc
     8461 gctttttact tcagcgagac tgatgccata ctgcgccagg cgctgagggt cgataaccac
     8521 ctgatactct ttcaccacac cgcctaccga cgccacttcc gcaacgtcag gaatggtttt
     8581 cagctcatat ttgagaaacc agtcctgcaa tgagcgtaaa tcggcaagat cgtgcttacc
     8641 gctgcgatcc accagtgcat attcatagat ccagccaaca cccgtggcat ctggtcccag
     8701 ctcggcgctg actcccgcag gcagcttacc ctgtacctgg ttgaggtact ccagcacccg
     8761 cgaacgcgcc cagtacggat cggtgccatc ttcgaaaatg acatacacat aagagtcgcc
     8821 aaactgcgag aaaccacgca cagtcttcgc gccaggcacc gacaacatgg tggtggttag
     8881 cggataagtc acctgatttt caacgatttg cggtgcctgg ccgggatagc tggttttaat
     8941 aatcacctgc acatcggaga gatccggcaa cgcatccact ggcgtattaa tgatggtcca
     9001 ggtgccccag atgctcagaa acaacgcgcc catcagcacc aaaaaacggt tcgccaccga
     9061 gcgacgaata atccattcaa tcattggtta ttccctcaat gcgcatgggt agcactttca
     9121 gagcgcatcc gctccagtgc gccagaaata ttggcttcgg aatcaatcag gaacaggccg
     9181 ccggaaacca ccttttcacc ttccgccaga ccagagcgta atgcggtgac gccttgcgat
     9241 gcctggaaaa cagcaacgcg tttcggtaca aagcgcccgt cggcatcaac ggtaatcacc
     9301 cgctgttcgt tgccggtatc aatcagcgcc tgtgacggaa tgagcagcat cggttcgctg
     9361 gcggtgttga gttgcaacca ggcgttcatg cccggtttta gcgcctcgtc ggcgttgtcg
     9421 acttccagac gcagctgcag cgtgcgggtc gcggcatcca cgccaggtag cagcgtccat
     9481 ttgcggatgg tgagtgtttt attcggtcgc gccggaacgg tgagggtaaa ctgcgaggca
     9541 tctttcacca gccaggcgat ggactccggg atcgcagcag tgacccacac cgggtccata
     9601 ccctgaattt tcgcgaccac gttatctttg gcgatattca ttcccgcgcg cagatcaaac
     9661 gcggtgatca cgccatcaat gggcgctttg agcgtaaagc gagtctggat tttttgcgtg
     9721 gcgatcaggt ggcgaatatc cgcctccggc attcccgcca gtcgcagccg ctcaagaatg
     9781 ccttcagtct gggtcgccgt accgccggtt tcgcgcagca gtaaatactc actctgcgct
     9841 tccacccagt cgggaatggt caggtcgaga agcggtgtgc ccttttgtac tttatcgccc
     9901 acggtaagcg gatacacctt gtcgataaac ccggcagcgc gagcctgcac aatggcatac
     9961 tgatactcgt tgtaactgac attcgccggg aaactctggg caaaagtcag cggcccgcgc
    10021 gtgacggtag ccgttttcac ccccagattc tgcgtctgag tcgggtcaat gcgcacacca
    10081 gacgcagaac tctcttcatc ggcatatttc ggcaccagat ccatatccat aaacggcgat
    10141 ttacctggtt tatcgaaccg cgtattggga tacatcgggt cgtaccagaa taagatttta
    10201 cgttctgcgg tcggcatttt ttctgcgtgc ggttccgcct gttcaaacca ggtaaaacct
    10261 gccgctgaaa taataccgcc cgcgatcatg ctgccgataa taagcgcgat ttttttcatc
    10321 tcattaaacc tgggttactg gctgacttta atatcctgta ataaagaaag gttgccctgc
    10381 tggacaaaat taaacgccac tttgtcgccg gttttaattt cactcatttt cgtctgcggg
    10441 gtgatggtaa agcgcatggt catctccggc cagttgacgg cagcaatcgg atcgtgatgg
    10501 atggtgattt ttttgctttc cagatcaaca ccttttacca caccggtggc actaatgacc
    10561 tgtggttgtg cttcgctcat ggtttcatga tgatgttcgt tagcctgggc attaaagcca
    10621 ataacggtaa acagactgaa cattgcgact tgcagtgctt ttttcattta ttctctcctg
    10681 gagttaaaaa ttattgctgc caaccgcccc ct
//
LOCUS       DADLSA010000129        10495 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.129, whole genome shotgun sequence.
ACCESSION   DADLSA010000129 DADLSA010000000
VERSION     DADLSA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.129"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            49..579
                     /locus_tag="JN691_003216"
     CDS             49..579
                     /locus_tag="JN691_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7244585.1"
                     /translation="MSITGVNIGRLFREGFSNYDPIGVLNAMASQRTKEARGGELQIN
                     ELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFT
                     EVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAV
                     QEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="JN691_003217"
     CDS             597..1235
                     /locus_tag="JN691_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7244586.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDADKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="JN691_003218"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="JN691_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAY7244587.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JN691_003219"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JN691_003219"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HAY7244588.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="JN691_003220"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="JN691_003220"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAY7244589.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JN691_003221"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JN691_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAY7244590.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="JN691_003222"
     CDS             complement(5552..6613)
                     /locus_tag="JN691_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAY7244591.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="JN691_003223"
     CDS             complement(6610..8007)
                     /locus_tag="JN691_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAY7244592.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLGQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="JN691_003224"
     CDS             8163..9161
                     /locus_tag="JN691_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY7244593.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10177)
                     /gene="ompG"
                     /locus_tag="JN691_003225"
     CDS             complement(9272..10177)
                     /gene="ompG"
                     /locus_tag="JN691_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HAY7244594.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(10222..>10495)
                     /locus_tag="JN691_003226"
     CDS             complement(10222..>10495)
                     /locus_tag="JN691_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HAY7244595.1"
                     /translation="TQESLHKPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFML
                     YTTVGGHELVVRAGALNDYHAGENITIHFDMTKCHFFDAETEIAIR"
BASE COUNT         2641 a         2811 c         2486 g         2557 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgaacaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tgccgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc ccacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca gcgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagcgtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtgccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaggcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cccttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcgtc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgccccagtt tggactcgcc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagccggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gaatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc acgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actccagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac cacgttccac gtttacccgc gctataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgttctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttcattat tgttccttgt attatcctgg
    10201 gggaatgcct tccccctgta tttagcgaat tgctatttcc gtttctgcat caaagaaatg
    10261 acatttcgtc atatcaaaat gaatagtgat attttctcct gcatgataat catttaacgc
    10321 accagcacgg accactaact cgtgcccccc aaccgtggtg tagagcataa attccgcacc
    10381 ggttaattct gccacgctaa ttttggcgga aatgttattt tcctcttgcg cgtccggatg
    10441 aatatcttcc gggcgtattc ccatcacgat gggcttatgc aaactttcct gtgtt
//
LOCUS       DADLSA010000130        10467 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.130, whole genome shotgun sequence.
ACCESSION   DADLSA010000130 DADLSA010000000
VERSION     DADLSA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.130"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(43..402)
                     /locus_tag="JN691_003227"
     CDS             complement(43..402)
                     /locus_tag="JN691_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HAY7244596.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(399..674)
                     /locus_tag="JN691_003228"
     CDS             complement(399..674)
                     /locus_tag="JN691_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAY7244597.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(733..1857)
                     /locus_tag="JN691_003229"
     CDS             complement(733..1857)
                     /locus_tag="JN691_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAY7244598.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(1857..4592)
                     /gene="rbbA"
                     /locus_tag="JN691_003230"
     CDS             complement(1857..4592)
                     /gene="rbbA"
                     /locus_tag="JN691_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAY7244599.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(4589..5656)
                     /locus_tag="JN691_003231"
     CDS             complement(4589..5656)
                     /locus_tag="JN691_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7244600.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(6022..7644)
                     /locus_tag="JN691_003232"
     CDS             complement(6022..7644)
                     /locus_tag="JN691_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY7244601.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPSA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(7906..9495)
                     /locus_tag="JN691_003233"
     CDS             complement(7906..9495)
                     /locus_tag="JN691_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY7244602.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIPLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            9992..10467
                     /locus_tag="JN691_003234"
                     /pseudo
     CDS             9992..10467
                     /locus_tag="JN691_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908812.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
BASE COUNT         2703 a         2587 c         2316 g         2861 t
ORIGIN      
        1 cctgtaaagg cagatattct gcgtaatgta cgtcgctaaa atttataaat gattcaaacg
       61 ctcattcagt gtttcaataa tataggtatt aacagagact tgttgctgcg cagccgcatt
      121 attaaggcgt tcactcagcg attctggata gcgtaacgta aaggtcttaa tcttttctgt
      181 acgcgcatac ggctcaatac ccgcagcttt acaatcctca agatactctc gcagggaaag
      241 ctctccttct ttttgcagcc cctgaatact gtcagagaca aaatcacaat aacctgacaa
      301 gcccaggaat ttgccacgaa atgcattcag ttctggcacg taggtaataa cagctggttg
      361 cccggcaatc tccatagagt ttggcgtctt cagtttgatc atggttttac ccctatactt
      421 agtaaccagt cacgcacgct ttctacagcg cctttatcgg tatctggcga cggatgaggc
      481 cgatggaaac acgcaacgct catatttagt atgaacttgc aacgcgaacc tcttccttcc
      541 ttaatttctc cgcctaatgc tttaaccagt gactctatat cagaccactt gatcccttga
      601 ggaacaggag ttttaaatat ttgatccaat gtattttttt gtttcttccg taaagcctgt
      661 accttggttc tcatggcatg tccttatgac ttcactttat gacatcacag tatgaagtca
      721 agtttccctt ccttacgcca ttgtcccaat cgtcttcctg aatcgcagca gtgcgatggt
      781 gaaaaacgcg ccgccaattg ccatcagcgt caggaactgc ggccagacga tttcgaatcc
      841 ggcaccccgg tagaggatgg cctgcgcgag gctaacaaag tgtgtcgtcg gcatggtcag
      901 cataatgtcc tgcaccatct gcggcatact ttcgcgcggc gtggatccac cggaaagcat
      961 ttgcagcggc agcagcacca gaatcaccag cagccccagt tgcggcattg aacgcgctat
     1021 cgtccccata aaaatgccga ttgacgtggt ggcaaacaga ctgagcgcca cgcccagcat
     1081 aaacagcggg atcgagcctt caatcggtac gcccagtaca cctttcacca tcagcaccag
     1141 cgataatccc gataccacca gcaccaccag ccccatcgac cagatcttcg ccatcatgat
     1201 ctcaaacggc gttatcggca tcaccagtaa atgttccacc gtgccgtgtt cacgctcgcg
     1261 gatcagcgcc gatccggtca atacaatcgc cagcatggta atgttgttga tgatcgccat
     1321 caccccgcca aaccacgcgg gatcaaggtt cgggttaaag cgcatccggg tttccagcga
     1381 taccaacggt tcgctgttat cacggtagcg cgcgacaaag ctgttcactt caccgttgat
     1441 aatattctgg atatacccat tgccggtaaa tgcctggctc atgcgcgtgg catcgacgtt
     1501 cacctgaata tccggctggc gtccggcgag gacatcacgc tgaaaattag gcggaatatt
     1561 tatcgcgaag gtatagcgtc cggcgtccag tccggcatcc atctcatcgg cggtgatcat
     1621 ctccggtggc aaaaaccacg gacgatagaa gctgttaacg atccggttcg ataactgcga
     1681 ttgatccata tcggcaatgg cgatcggcgc gaggttcaac gatcctggcg taacggtcgc
     1741 tgacgaatac accgacaccg taaacgagaa gacaatcagc gtcagcatcg ctttatcacc
     1801 gagcagactg cgcaactctt tgatacccag attaaaaata ttgcgtaaat ggcgcatcat
     1861 ccctcctgtt ttttcagcag caggatgctt aagcccatca ccagcgggat ggctatcagc
     1921 aacgggataa aaagttgcca caaatcagtc agatccagcg ctttcgagaa cgttccgcgg
     1981 gcgatagtca gaaaatgact ggtcgggtaa acctcgccga tccaacgtcc aggcccttcc
     2041 agcgaagcta ccggatcgat catcccggaa aactgtgtcg ccgggatcaa tgtgataatc
     2101 gccgttccga aaatggcggc aatctggctt ttcataaagg tggagatcag cagccccatt
     2161 ccagtggcaa tgatgatata cagcagcgcc gccagggtga gcgtcaggaa actgccttta
     2221 tgcggtacgc caaacacaaa caccgacagg ccgcagagca ggaaaaagtt cagcatcccc
     2281 agcgcgatgt acggcagctg tttgccaagc aaaaattcgc tacgggtggt gggggtcacg
     2341 taaaggttga taatcgaccc tagctctttt tcccgcacca cgctaagggc gcttagcatt
     2401 gacgggatca tcatcagcag aagcgggatc accgccggaa caatcgctgg caggcttttt
     2461 acgtccgggt tatagcgata acgcgtctca atgttcatca gcccgctttg gctggcgggt
     2521 gtcgattgtc ggctcgccac atcctgaagc caactctggt gcattgcctg cacgtaacct
     2581 tttaccgttt cggcacgact gggcatcgct ccgtcgatcc agacgccgag ttccacaggc
     2641 gtaccacgcg cgatatcgcg cccgaaattg ggcgggatct caatggccac cgtgatatcg
     2701 cccgcacgca tccgacgatc aagctcgtca taactggtga gcggcggctg ttcgataaag
     2761 taacgggaac cggagaggtt gagtgtccac gcctggctac tgacggtctg gtcgcggtcg
     2821 agcaccgcaa agcgcaggtt ttccacatcc atactgatgc cgtaacccat tatcagcatc
     2881 aggatcaccg ttcccatcag cgccagcgtc gaacgtactg gatcgcgtcg cagttccagc
     2941 gcttcgcggc ggctgtagct aaacagacgg cgcaggctaa atccctgacg cggcgcgtgg
     3001 gtggtgtcgt gtaccacggg cggcgcttcg gcttcgttgc tctgccctgc cgcttcctgc
     3061 aaataggcga taaatgcctc ttccagactg gcggctccgc gtttctcaac cagttcctgc
     3121 ggtgtaccgc tggcaagcac ttttccggcg tgcatcagtg agatgcggtc gcaacgttcc
     3181 gcttcgttca taaagtgggt ggagatgaag atagtcactt tgtcctggcg cgagagatcg
     3241 accatcaact gccagaacat atccctcgcc accggatcga caccagaagt aggctcatcg
     3301 aggattaaca tctccgggcg atgaatcacc gccaccgcca gcgaaagccg ctggcgaatg
     3361 ccgagcggca atgactccgg cagaacatct tcaacgtcgt tgagcttaaa acgctcgctc
     3421 atttcagcca ctcgcgcggg aatttccgct tccgggatgt gaaacaaacg ggcatgtaac
     3481 tccaggtttt gccgcacggt gagttcgtta tagagcgaaa acgcctgcga catatagccc
     3541 acacgacggc gggtatcgat atcttttgga tcaaccggtt gcccaaatag ccacgcctca
     3601 ccttcgctgg cgggcagcag tccggtaagc attttcatgg tggtggattt accgcagccg
     3661 ttcgaaccaa ggaatccaaa aatctccccg cgtggaatgc ggaaattgac gtgatcaacg
     3721 gcaacgaagg aaccaaaacg catggtcaga tcgcgcgctt cgatggcaat ctctgcgttt
     3781 tcaggttgat acggtgggat cactaccgcc tgatgcgcct ggcgttgcgc ttgcggtaac
     3841 agattaataa atgcttcttc cagcgtggcg ctttgcgttt gctgccgcag ctcttcggcg
     3901 ctgccggttg ccagcacttc tccggcattc atcgctacca gccagtcgaa gcgttcggcc
     3961 tcttccatat aggcggtggc gaccagcacg ctcatattgc tctgccgctg gcgaatactg
     4021 tcgatcagat cccagaactg ggcgcgggag agcgggtcaa cccccgttgt tggctcatca
     4081 aggatcaaca gttccgggtc gtggattaac gcgcagcaca gcccaagttt ttgcttcatc
     4141 ccgccggaga gtttacctgc cggacgatcg cgaaacggtg ctaacccggt gctggtcagc
     4201 agctcattaa ttcgcacttc ccgctccgct ttatcgtgac cgaacaggcg agcgaaaaaa
     4261 tcgacatttt catacaccga caaggtgtgg tagaggtttt tgcccagccc ctgcggcatc
     4321 caggcgatgc gcgggcagac gtctcggcga tgcttcgggt cgtgcatatc gccacccagc
     4381 accaccacat tgccctgctc aatgacgcgg gcaccggaaa tcagtgacaa taaactcgac
     4441 ttcccgacgc catccggacc aatcagcccg accatgcaac gggccggaat atcgagagtg
     4501 atattgttca gcgcaacggt ttttccataa tgctggctca cgcccgccag ttgcgcgaca
     4561 ggcgggacgg gaaccagttc cagatgcgtc attgcggcaa cctcaccacg aggtcgtcag
     4621 gccacggaag ttcttcattc acccgcaccc acgctacgcc cggtaaaccg gttttgacat
     4681 attccagatg ctgctggagt aattccggtg ggatacgcgc tttgacgcgg aacatcagtt
     4741 tcagccgttc atcgctggtt tcgacggttt ttggcgtgaa ctgggcgaca ctggcgacaa
     4801 aactgatggt tgcaggaata cgcagatctg gcgcggcatc gaggatcagc cgggcttcac
     4861 cgcccagttt cagcgtgccc gcctgttcgg ttggcaggaa gaaggtcata tagacgtcgc
     4921 tgagatcgac catattcagc acccgaccgc ctgccgccag cacttcccct ggctcggcaa
     4981 cccgatactg cacgcgtccg tcacgcgggg ctttcagttc actgtcatcg atatctgcgg
     5041 caatgcgccg ttcagtggct tgcgctgctt cgacccgagt ttgcgcctga atgatattgg
     5101 tgcgtgctgc ttctatagcc gctttagaag ccgaaacctg ggctttcgct gattccagcg
     5161 cagctcgggc gctctcagcg gcggcgcggt cgtcatccag ctgttgcgca gaaatagccc
     5221 ctcgttgggc gagcgagcgg gaacgcgtat gacgtttggc aacggagtcc agttcggcct
     5281 ggcgttgatt gaccagcgac tgtgcggcac gagtttcact ttgtcgttgc tccagcaaag
     5341 cctgcgcggc ggcaacggcg ctttgtgcct ctttgatttg cgcgatggct tccagtcgct
     5401 gttcctgcaa cacgcgagta tccatcttcg ccagcacttc gccttcgcga acaaactggc
     5461 cttctttcac cagaatggtg tcgatacgcc cggcaatttt gctggcgata tccacttccg
     5521 tcgcttcaat gcgcccattg ctgacagcaa agccttccgg cacacccgcc gggcgcaaca
     5581 gccaccacgc cacgacggca gccaccgcca gtgccccgac aacccaccac gccagatggc
     5641 gcttactctt atccataatc gacccgccat aatccctgta agcaaacgac gcgtgttaca
     5701 actgccgccg aacgacgttc agcggattct tccatccggg ataagaagga aacgacaaat
     5761 acgaccagcg cgcagagcat tgagcgccaa cggggaaggc ttagtgaaaa cgggtagtaa
     5821 tgttgtcatc atcagcattc ctggccgtaa atgaaacacg catactcata agtgtgatct
     5881 ataaaactgg ctgacaaaac ggtatccatc ctgttggatc gcgtttcacg cctgttcagt
     5941 tgcaggaaca gagcattcct ttttacgttt tatcaacagt ctcaaccaga tagtaacatt
     6001 aatattcatg ttgtctatgg ttcagagctg taacattgtg cgccttctaa tcaaaggcat
     6061 aagtccattt ctgttttttt catgctggta agacttccag ggagtggtta tctctggccc
     6121 tgcactaggg ttaatttggc aattatgcat gctgaacatt cttccagcat gtttactgtc
     6181 acgggcgtta agacctaaaa ctgtccccgt ttcacctatg cgttctctcc caccatttcc
     6241 gttatgtgca acaacttgat ttatacctaa catttgtgaa catgctaata gtgcttcaag
     6301 tttaggtctg taatctgtcg aaacagaaaa ataacgatta aaggatcgac aagagatagc
     6361 ccggctagtt ttaaactttt tgccattaat tatagcatga tgctttttat taataatttc
     6421 cgctaattcg accggatcga gagcattggt catttcatca atagagacag ttatcggtcc
     6481 tataatgtaa cgttttcgct gatcatcaaa agttatccca tgatgatttg ccattatttt
     6541 gtatttgggg tcatagaaac atacaggata atcttttatc ccgtcgtagg tatcctcatc
     6601 aagtggtttc atgcgcgtga agttttgcat atagttacca ttgcaattcg tttcatgatt
     6661 tccagctaat acatgaacat tttcattgat actcattatg cgttttaaca aatcgatgat
     6721 aaattgatcg ccacagatat tactaaatcg atcgccggtt tggtcgccaa gaaacgtaca
     6781 tggcgtgact gcgtctttat taataaccaa ataaggctga agactttcta aaacattatc
     6841 tcttctgttc agtatactga ttattgtatt aacattagtg tcaagttgct cattaaagtt
     6901 ctcccttagc aatacgttga gaacttcatc ttctatatta agcaactggc taaggaaatt
     6961 ttctccttca gccatgattc ccaaatgtcc tgttgcaaaa agcgcgtaca aaactgcacc
     7021 gactgagcca tcggtatcgc caaaataggt cggcctggta cttgtgggat caacaggtga
     7081 acaattggag aaataggttc tgtcagcgaa ctggggtttt ataattgttt cgaatttaat
     7141 atcaaataca ttgtattgcg atatttccca gttccagatg ggtttgtacg actcagtaac
     7201 tgcaacatca ttagcattat catttactgt catattatta agtaaataaa caatgctgca
     7261 ccaattaata tcggtattat caagagggca accgtaattt ttactattaa ttgtcacaat
     7321 aaactgataa cagttattcc tacgtagaat atgagcgcgc atgcccgata gcatactata
     7381 tttttctgaa tgattcgttt gtatggtaaa tgaaattttg tcatcttgaa tagattgttg
     7441 gaaacgttct atttgccaat catcaatttt ttccacaact tcaccgagca ttgtcctggc
     7501 aatacttgat atatcatcac ctataccatt tgcaatatac ccaaacagtt ctttagtgaa
     7561 gttggtaaca tgctcttgca ccatatcatt tgttgcgatt gtcgttgtac tgccattagt
     7621 gcctgccaca gttccaattt tcatctttta cctcatatta ttcaccgcga ggaatacata
     7681 gttaacattt tatcttatga catacacctc atattcgatg ggaaaataat tatattttgc
     7741 acaaatcatt cagccttaaa gcataatatg aaatttctta tctcatagca gagaaagtcc
     7801 ctaaagattg tagaggcgtc atcagtacat acctctacaa tcgattaaga aaacctatga
     7861 aatactggca aaattaaaaa gagataccac agcactcatg aagttttatt gaggttgaaa
     7921 acaactaagc cctgggacac cttttttatc accacgttcg ttaggtgcta tcttccagga
     7981 atttagcccc atggtataat tatcaatttt ttgatgctgg tctaccccta atccataatg
     8041 gccaaatttc tgtttagttt ttttgagcgt ctgtccacat ttgaagatat cttcaggttt
     8101 tggtctgaaa gcagtagtgt tattaaaata attttcatat acaggtgtgg gtgctatagc
     8161 attgcttcta attagatgga gaagatgatc atccatgtgt tgtttatgtt ttttattaaa
     8221 tatatttgct aattcctcag gatcaattgg gtctttcatc tgattaaatg gaacctgcaa
     8281 agcaccgaga caataacatt gattttcttc atttcttatt atcccgtgat gactggttaa
     8341 aactttagtt tctgagttat aattacaaac atcgagcgtt ttaattaagt tatacgtatc
     8401 accggcagag agtttataat tttctagtcg tgcagtatag ttaccattaa aattgatttc
     8461 atgatttcca gccagaacga tgacattttt attaatacgg ctatctttat taccctccat
     8521 atttcgcata gaatttagca aagtaaggat atatttatca ccaaaaatag tactaaatct
     8581 atcgcctgta tgatccccaa gggggataca aggggttatc gcatcgtcct ttaaaaaaac
     8641 atgttgatgc aactcattca atatcatgtc tctcgtattt aataaattgt agagcaattt
     8701 attattttta tgtatgtatg cagatgaagc catgtcttcg tgttttaata attcacataa
     8761 taaactccaa cccttttccc tgatgcctat atgccctgaa acgagcaatg aataaagtac
     8821 agcgcctact cttccatctg tatcgccaaa ataagccgtg ttggcatttt gtgggttaat
     8881 cacaccacat tgtttataaa agtctatttt agaaaacaca gatttaacat agctatcaaa
     8941 cttatcctta ggaaacgtct ctttagagac atcccaacgc cagtcagctt taaaattacc
     9001 gctaagaaga gtaaagaaat tttcaggtat gatagaaagc ccattaactt tataaattat
     9061 gtctgattta tctccggaga aattaataaa ataataacca tccttacact caatcttaac
     9121 tgtaatggga gacgataatt ttatcacatg gttattgtct ggtataattg taaagataaa
     9181 cgtgttagta gtattatcgg caaaactatt atattcttga atatctatag atggctcact
     9241 attgtaaaac catattttct ccatctgaag actatgttca tttaccctcc agtgaccatg
     9301 ttgcaggaaa tagatgatat gcatgccaaa atcgttatct atttgcgaat cattatccag
     9361 tttatcaatt tcaactatct ttgaacgatg tgttttatca accttgctat ggtgttgatg
     9421 attattaact ttatctgaat gcttatcttt atggtttgaa gggatgtact taaatatatt
     9481 gtcaattgct ttcataaata acccgcgata tttttgacct gttagtaaca aataaatgat
     9541 acatgtctga aagacatatt tacgacgata taaaacaata atacttatat gttcgctgat
     9601 tttaagcacc gagactatcc ggtcttttac taaataatgg aaaacaatcc atccctggaa
     9661 gacaattgta aaaaaaaaca acagaataag aattaatctt aggataaata tttatttatc
     9721 atggcctttt gaccgctaaa aaatggcact cggcacagta aaccccgcta tagttatgct
     9781 atgcatttgt ctttaatgat atctcgtcgt tatattaaca ggctgaaatt atcattaatg
     9841 cacatttcaa tattagcagg gatacctgag gagtttaaag tgaacatata tatcgggtag
     9901 cttttcaaat taatcccttt gattataggc ttaatttgca tcgcgttagg tggctttgtg
     9961 ctggaaagtt cagggcaacg tcgcgggtca tgtgctgatt tctctggcgg ccatatgcct
    10021 agcattattc actaccgcat ttattatcat ttcgcagctc acgcgcggcg ttaatacgtt
    10081 ttacaataca ttgttcccca ttattggcta tgcggggtca attatcacca tgatatgggg
    10141 ttgggcactg ttagcaggta atgatgtgat ggcagacgag tttgttgccg gccatgttat
    10201 ttttggcgtt ggtatgattg ccgcatgtgt atcgacggtg gcagcgtcgt ccggtcactt
    10261 tctgctcatt cccaaaaatg cagcggggag caagagcgac ggaacaccgg tacaggctta
    10321 ttcttcatta atcggtaatt gcctcattgc cgttcccgtt ttactcaccc tgctcggttt
    10381 catttggtct attacgctgt tacatagtgc cgacataact ccgcattatg tcgccttggg
    10441 ttaaccgcaa tctgtgcctg tctaatt
//
LOCUS       DADLSA010000131        10457 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.131, whole genome shotgun sequence.
ACCESSION   DADLSA010000131 DADLSA010000000
VERSION     DADLSA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.131"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            90..644
                     /locus_tag="JN691_003235"
     CDS             90..644
                     /locus_tag="JN691_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAY7244603.1"
                     /translation="MAKLTDKQELFAREYLKDLNATQAAIRAGYSEDSAAVQGCKNLI
                     KANIAKRIAELKAERNEEVGIDAAYVLRRLVEIDQMDVLDILLQNGELKPIKDWPKVW
                     RTTLSGMDVVEMVSADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNEVTGADG
                     GPVRTEITKLTPEQAAEVYRKMMG"
     gene            647..2266
                     /locus_tag="JN691_003236"
                     /pseudo
     CDS             647..2266
                     /locus_tag="JN691_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130781.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
     gene            2266..3807
                     /locus_tag="JN691_003237"
     CDS             2266..3807
                     /locus_tag="JN691_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HAY7244604.1"
                     /translation="MWPFKRKEPSAPAVPDKQPEPQPPEINDEVIASVRQKPRREFVR
                     YEPPPGVIPAPVRDAVLAMDATPYDTLNSHYPDFVYGGFPGYPYLALQAQLPEYRRMV
                     SVLAEEMTRKWIKVKAVGEGDDSHASRIAQLTDALEHYNVQDVFRLAIEHDGFFGRGQ
                     IYIDVRSPSGISAWTDPAELGSGLFISDKKIPKGSLLGLRVIEPVWTYPGIYNADNPL
                     SDDFYRPSEWYVMGKTVHASRMIDLISRPVPDMLKPAYNFGGLSLVQIAEPYVDNWLR
                     TRDSVGDMLHSFSLSGIKTDMTQVLSGKRDPNYAKRAELFNRTRDNRGLLMLDKTNEE
                     FFQFNTPLSGLDTLQAQAQEHMFFVSAIPSVKFAGLSPTGLNASSEGEIRVFYDTIAA
                     LATRLLKKPLKKVLDIIQLSEFGDIDPDITFEFEPLHELTREQLANIRKTEAETDQIY
                     ESTGAVTNNEVRERLATAQDSPYSGIDLSGDIEIDDTEIDNTEENPPQGPNADPEADF
                     TQRGD"
     gene            3848..4537
                     /locus_tag="JN691_003238"
     CDS             3848..4537
                     /locus_tag="JN691_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000552018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAY7244605.1"
                     /translation="MHNSVLYWLRAEYRKTDLAQDASPVNLMRGAMQQLARRWQKNFD
                     EMALRLARRFAGDVLKNSDASLYTALRDAGFTVPFRMTTEMNTALQASITENVNLIRS
                     IPQQHLPQVETLVMQSVGRGRDLKTLTDELEKRYGITRRRAALIARDQNNKATSVMQA
                     ARQRSVGITEGVWRHSRAGKTWRPSHVKANGKRFDLRKGMFLDGKWVLPGEEINCRCG
                     WEAVIPGLEKK"
     gene            4534..5883
                     /locus_tag="JN691_003239"
     CDS             4534..5883
                     /locus_tag="JN691_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HAY7244606.1"
                     /translation="MIITEMLAFDRASVRQFDKVGRFQIERSNLSKANVCGYFGYEIP
                     GAEALGLDPKKLYQLYRDPDELRKAVSTFNNIPVLCRHKPDYPGAPAREYRVGTTHAN
                     SEFDGTYLVNGMSIWDNSAIAGIETDEQREISSSYAYVADMTPGTTPDGEPYDGVMRN
                     IVGNHVALVGDGRVGSDCLVMDSLPQELKRMKLSKKEVAVLTALGTYLAPRLAQDAAP
                     GDLLRLMAQYKRPAAIASAVKTAYSERLVQDMDIEPVELAQLMESAEAVPELVGDDDT
                     GLTDESKAFDTDSPMESVLALLSGKVPDDVLEKIKSALSPTTDEAPETKEADVKPDDV
                     KVDKPAMDAAIRLATDQATKRAAENFRAVRVAEAEVRPLIGDVVAMDCAEDVYRTALE
                     QTGIDIQGIHPGAYRSMVKFAVEQKQTAKSSPRVAMDQASASTFAADFPGAKLKRGY"
     gene            5885..6367
                     /locus_tag="JN691_003240"
     CDS             5885..6367
                     /locus_tag="JN691_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244607.1"
                     /translation="MNTFQTHMNQYPAPGIPVAFASANPHASYVAGDGGLITGPDGLV
                     IARFAWVTKGVAANKGAGAPAGFVPRDGQASIVEWLAGDSNTIYPGRECTLMVSGDFW
                     ALTTTTATIGQKVFASLATGEIATGAAGTSMAGFVETWFSVASAAAAKEVIKISTWSK
                     "
     gene            6367..7395
                     /locus_tag="JN691_003241"
     CDS             6367..7395
                     /locus_tag="JN691_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HAY7244608.1"
                     /translation="MNKFKQHYATASRDYGIILPGAQAYLPKEYAADYGLAMDAQPAL
                     VTAANSGIPAYFTNYVEPELIRVLVTPMKAAQILGETKKGDWTTLSAQFPIAESDGEV
                     SSYGDYSNNGVVSANVNWVPRQSYHFQTFTVWGERELEMYGAARIGWAAELNVASALT
                     LNKFQNKSYFYGIAGLTNYGLLNDPSLSAPITPDTVDGKLKWDDKDGQGVYDDIVKLF
                     KQLVKQTNGHIERTDKMKLCMSPLAEVNLTKTNQYRVNVSDLLAKNFPAMTIETAVEY
                     TSDAGELVQLIAERLGEQDTGYCAFTEKMRAHAVVADTSSWKQKKSAGTWGAIIRQPL
                     AYAQMLGV"
     gene            7399..7746
                     /locus_tag="JN691_003242"
     CDS             7399..7746
                     /locus_tag="JN691_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244609.1"
                     /translation="MAEMVTVGCKLPNGLVLEVGQKRVQVAGWRNNAVKIVGGYGLTQ
                     VDKVFFDAWLAEHGQQPYVKNGVIFAQDKANSAVSQATEQKAVKSGLEPLPQKNPAPG
                     VNRNDEVMGKPQE"
     gene            7752..8210
                     /locus_tag="JN691_003243"
     CDS             7752..8210
                     /locus_tag="JN691_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HAY7244610.1"
                     /translation="MGTVTFDCRMFVSLYPEFSMVTPELLTAVFNQATALYLDNTDDS
                     PVTDLKEREQLLFLLVAHLCALRGLGNGRDGQAGLVGRITSASEGSVSVSVDNSGSND
                     ASWWYLQTPWGADYWQATAPYRSMQYHSGGAPSRYPGHYYRGYGRGVDGK"
     gene            8200..8784
                     /locus_tag="JN691_003244"
     CDS             8200..8784
                     /locus_tag="JN691_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244611.1"
                     /translation="MVNKVTGGKQFRQKLKQVAANLSLGKKLKVGFLEGATYPDGTSV
                     AYIAAVQEFGGRAVIPAREQTLHFRYNEKTGETGHRFVKAGKGNFVQDVVIPEHTVTI
                     PPRPFFRKMIEHKSPEWGEKMATLLRANDFDTATALVCMGEHIKGQLQMSVRDWESPP
                     NAASTARQKGFNNPLIETGHMMDSVDYSVDGGKK"
     gene            8781..9146
                     /locus_tag="JN691_003245"
     CDS             8781..9146
                     /locus_tag="JN691_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244612.1"
                     /translation="MNFHGIVSGAVRRVNPFTDALVYRSRGSTQQADYSRVPEYEDPV
                     SVRVQKQAVTQADLRHLDNLNQQGAFATLYTDGNWCGLNRARQQGGDKFVIGDETWLV
                     IEVPEIWPDWTRVIVCLQV"
     gene            9131..9676
                     /locus_tag="JN691_003246"
     CDS             9131..9676
                     /locus_tag="JN691_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244613.1"
                     /translation="MPSGVTLSVTESALYQALGDYLRGLFSDAGIERTQQNQVPMPQG
                     NFITMTGIDVTGLSTAVVAYSAPAQAGEGSQHITRTTKWRCQLDFYGPHAADNAQVLA
                     TLFRSEFSVRLFRQGGGLISPLYCSEPFNTTFPNGQQQYEPRWTLDIQMQINPVVTTS
                     LMFFDNVITRTTEADNANSTQ"
     gene            9657..>10457
                     /locus_tag="JN691_003247"
     CDS             9657..>10457
                     /locus_tag="JN691_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HAY7244614.1"
                     /translation="MPIPLSKDVQINPGVLAVAGNAVDLNGLLLTSNPLLPVGTVVPF
                     SSPDDVAAYFGALSDEYARAQLYFQGFKNATRTPGQLLFSRFSPAPSAAWLRSGSFKG
                     ATVEQLQKLSGTLMLSINGKSVSIEVNFNGVASFAAAATALQTALTAAVATVVFDTTQ
                     NAFVITVAGAKPGSTTITFGSGSAAELLKMTSNTGAVISRGAPVSDVPDTMAAIKAAS
                     QQWAGFSTVSEVTDEQHLAFSAWANGQGKRYFYVAWTTSGNARVKGSTE"
BASE COUNT         2535 a         2547 c         3033 g         2342 t
ORIGIN      
        1 ctatctacgg gtgggcttga taatggctta taccctacac gggataactt aactgatatc
       61 ccttttaacg gataaacgga gcacaaataa tggcaaagct caccgacaaa caagagctgt
      121 ttgcccgtga gtacctgaaa gatctcaatg ccacgcaagc agccatcagg gcgggttaca
      181 gtgaggattc tgccgccgta caaggatgca aaaacctaat aaaagctaat atcgcaaagc
      241 gcattgctga gcttaaagcc gagcgaaacg aagaggtagg tatagatgcc gcttatgttc
      301 ttcgtagatt ggttgaaatc gaccagatgg atgtgctcga cattctcctg caaaacggtg
      361 agctaaagcc cattaaagac tggcctaagg tatggcgcac aacgctatca ggaatggatg
      421 tcgtggagat ggtatccgca gatagcgccg cacttctgaa gaaaatcaaa tggcctgata
      481 aggttaaaaa ccttgagttg cttgggcgcc atgtttctgt tcaggcgttt aaagacaacg
      541 tcaaaaatga agtgactggc gctgacggag gaccagtcag aacagaaatt accaaattaa
      601 cgcctgagca ggccgcagag gtgtatagaa aaatgatggg ctaagtatgc cgttaccatt
      661 ccccttcgat tttaaacatc ctgattacca gatggttttt gaatggcgga tggaacgcct
      721 acagcgcatt cgccagaacc ctgaaatatt gcccgtattg aagcagtttt accgaaccaa
      781 tccggctcag ttcatcatcg actggggcat gacaacggac ccgcgtaata ttgattatgg
      841 cctgccggtg accattccgt ttttactctt ccctaagcag gaggagtgga tccactggat
      901 tatggaacgc tggagcaatc gggagaatgg tattaccgaa aaatcccgtg aaatggggct
      961 cagttggacc gcgatcggac tggcctgctc gctttgtctc ttcaacaaag aaatggttat
     1021 cggtttcggc tcccgtaaag aggaatacgt cgacagcacc ggtgacccga aagcattgtt
     1081 ctggaaggcg cgcaagttcg tggaaactct acctgtagag tttcgcggtt cgtggagcga
     1141 gaagaagcac gcgccatata tgcgtgttga gtttcctgaa actggcgcgg ttatcaaagg
     1201 tgaggctggc gataatatcg gtcgtggtga ccgtacgacc ctttattttg tggatgaggc
     1261 tgcttttctc cagcgaccat tacttattga tgccgcgctt tcccagacaa cccgatgtcg
     1321 tatcgatctc tcatcggtta acggcatgaa caaccccttc gcacagaagc ggcacagtgg
     1381 aaaaatcccg gtgtttacgt ttcactggcg tagcgatccg cgtaaagacg atgagtggta
     1441 tcacaaggag tgcgagaaaa ttgataaccc agtcatcgtt gcctaggaac tggatcttaa
     1501 ttaccaggca tcagcagaag gcatcctgat cccctcagaa tgggtacagg ctgcggttga
     1561 tgcacatatc agactgggca ttcagcccgg cggccaacgg cttggtgcaa tggatgtcgc
     1621 cgacgaaggg cgggataaaa acgcctgttc gctccgttat ggcatcctgc tgaatgacgt
     1681 acaggagtgg tcaggtaagg ggagtgacat ctatgactct gtggttaagg tttttggtct
     1741 gtgcgatgac tttggtgccg atgagtttcg ctttgatgag gacggattgg gtgctggcgt
     1801 tcgtggtgat gcgcgcgcta tcaacgaact gcgtgaagcg gagggcatct gtcaaatcac
     1861 cgccactcca ttccggggca gcgggagcgt gtttcatcct gaaaatgaag ctgttcccgg
     1921 tgataatggt aaaccggctc gtctgaataa ggactttttt gtcaatgcca aagcccaggg
     1981 gtggtggcat cttcgcaaat tattccgcaa tacatttcgt gcactacagg gtatggagta
     2041 tgacccggat gagattattt ccatcagcag cacaatggaa aataaagacc ggttgttgat
     2101 ggagctgtca caacctacct ggtcaaaaaa tgccaccgga aaaattcttg tggataagca
     2161 acctgacgga acgaaatctc caaacctggc tgattctatg atgattgctt atgccccgat
     2221 ggaaatgccc atcgtaattt ctgatgactt tatggagtgg atctgatgtg gccgtttaaa
     2281 cgaaaagaac cgtcagcgcc ggcggtaccg gacaaacaac ctgaaccaca gccaccggaa
     2341 attaacgatg aggtgatcgc ttccgtccgg cagaaaccac ggagggagtt tgttcgctat
     2401 gagccaccac cgggcgttat cccggcaccg gttcgcgatg ctgtgctggc aatggatgca
     2461 acaccgtatg acaccctgaa cagtcattat cctgattttg tttatggtgg tttcccgggc
     2521 tatccgtatc tggcacttca ggcgcagtta ccggagtacc ggcgtatggt cagtgtgctt
     2581 gccgaggaga tgacccgtaa gtggataaag gttaaggcgg tcggagaagg ggacgacagc
     2641 cacgcgtcac gcatagcgca gcttactgac gcactggagc actataacgt acaggacgtt
     2701 ttcagactgg cgattgagca cgacggcttt ttcgggcgag ggcagattta tattgatgtg
     2761 cgttcgccat cgggtatatc tgcctggact gacccggcgg agctgggatc cggactgttt
     2821 atttccgaca aaaaaatccc gaaaggttcc ctgctggggc ttcgtgttat tgaacccgtc
     2881 tggacgtatc cgggtatata taacgcggat aatccgctga gtgatgattt ttaccgtccg
     2941 tccgaatggt acgtaatggg aaaaacggtt cacgccagcc gcatgattga tctgatctcc
     3001 cgtccggttc cggacatgct gaagccggcc tacaactttg gcggcctgtc actggttcag
     3061 attgctgagc cttatgttga taactggctg cgaacacgcg acagcgtggg cgatatgctg
     3121 cattcgtttt cgctgagcgg gattaagacg gatatgacac aggtattaag tggtaaaagg
     3181 gacccgaatt acgcaaaacg cgcggagttg tttaaccgta cccgtgataa ccgcgggctg
     3241 ctgatgctgg acaagacgaa cgaagagttt ttccagttca acactcccct gagcggcctc
     3301 gacacccttc aggcgcaggc acaggaacac atgttctttg tcagtgccat accgtccgta
     3361 aaattcgccg ggctgagccc aacgggactg aatgcgtcga gtgagggtga aatccgggtg
     3421 ttttacgaca ccattgctgc acttgccacc cgtcttctga agaaaccgct gaaaaaggtg
     3481 ctggatatta tccagttgtc tgagttcggc gatatcgatc ctgatatcac gtttgaattt
     3541 gaacccctgc atgagctgac gcgagagcaa ctggcgaata tccgtaaaac tgaggcggaa
     3601 acggatcaga tttatgagag caccggagca gtgaccaata acgaagtacg cgaacggctg
     3661 gcgactgcgc aggacagccc gtacagcggt attgacctga gcggggacat cgaaattgac
     3721 gacactgaaa ttgacaacac cgaagaaaat ccgccgcaag gcccgaatgc agaccctgag
     3781 gcggatttca cccaacgcgg ggattgaggc ctggtaccgc agacaactgg ataatgccgt
     3841 cagtgggatg cacaacagtg tactttactg gctgcgggct gagtaccgta aaacagacct
     3901 cgcgcaggat gcgtcccccg ttaacctgat gcgtggtgcc atgcagcaac ttgcccggcg
     3961 ctggcagaaa aattttgacg aaatggccct gcggctggcg aggcggtttg ccggtgatgt
     4021 cctaaaaaac agcgatgcgt cactgtacac tgcgctccgt gatgccgggt ttacggttcc
     4081 tttccgtatg acaacggaga tgaataccgc acttcaggcc agcatcacgg agaatgtgaa
     4141 cctcattcgc tccatcccgc agcaacatct cccccaggtg gaaacactgg tcatgcagtc
     4201 tgttggccgg ggacgtgacc tgaaaacgct gaccgatgaa ctggaaaaac gttacggcat
     4261 cacacgacgg cgcgcggcgc tgattgcccg cgaccagaac aataaggcaa cgtcggtaat
     4321 gcaggccgcc agacagcgtt cggtgggtat cactgaaggt gtctggcggc attcccgcgc
     4381 gggtaaaaca tggcgtccgt cgcatgtgaa ggcgaacggt aaacggtttg acctgcgaaa
     4441 ggggatgttt ctggatggta agtgggtact gccgggcgaa gaaatcaact gccggtgtgg
     4501 ctgggaggcc gttattcccg gactggagaa aaaatgatta ttaccgaaat gctggcgttt
     4561 gaccgggcat cggtaaggca gttcgataaa gtcggtcgct tccagattga gcgcagtaat
     4621 ctcagcaagg cgaacgtctg cggttatttc gggtatgaaa taccgggggc ggaagcgctg
     4681 ggactcgacc ccaaaaaact ttatcagctt taccgtgacc ccgatgaact gcgcaaggca
     4741 gtttcaacct tcaacaatat tcccgtcctg tgccgacaca aacccgatta tccgggcgcg
     4801 cccgcgcgcg agtaccgggt ggggacgact catgccaaca gcgagtttga cggtacctat
     4861 ctggttaacg gcatgtccat ctgggacaac tccgccatcg cggggataga aacggatgaa
     4921 caacgggaaa tctcatcgtc atatgcctat gtggcagata tgacgccggg aaccaccccc
     4981 gacggtgagc cgtatgacgg cgttatgcgg aatatcgtgg gaaatcatgt ggccctggtc
     5041 ggcgatggcc gggtagggtc ggactgtctt gttatggact ctctccctca ggagctaaaa
     5101 cgcatgaaac tgagtaaaaa agaagtggcg gtgcttaccg cgctgggaac ctatcttgcg
     5161 ccgcgtctgg cgcaggatgc ggctcccggg gatttgttac gcctgatggc gcagtataag
     5221 cgtccggcag ctatcgccag cgcggtaaaa actgcttaca gcgaacggct ggtacaggat
     5281 atggatattg aaccggtgga gctggcgcaa ctgatggaat cagcagaagc cgtaccggag
     5341 ctggtcgggg atgatgatac cgggttaact gacgagtcga aggcatttga taccgacagc
     5401 ccgatggaaa gtgtactggc gttgctgtcc ggcaaagttc ctgatgatgt gctggaaaaa
     5461 attaaatccg cactgtctcc gacaaccgac gaagcccccg aaacaaaaga ggctgatgtg
     5521 aaacccgacg atgtgaaagt cgataaacct gcgatggatg cagcaatcag gctggcaact
     5581 gaccaggcaa cgaaacgggc tgctgaaaat ttccgcgccg tccgtgtggc tgaagctgaa
     5641 gtgcgaccgc tgattggcga tgtggtggcg atggactgcg ccgaagatgt ttaccgtacc
     5701 gcgctggaac agacggggat cgatattcag ggtattcacc ccggtgcgta ccgcagcatg
     5761 gtgaagtttg ccgttgagca gaagcagacg gccaaatcct ctccccgtgt ggcgatggat
     5821 caggccagcg catcgacgtt tgcggcagat ttccccggtg caaaactgaa acgaggttac
     5881 tgatatgaat acttttcaga cacacatgaa ccagtacccg gcaccgggta ttccggtggc
     5941 gtttgccagt gctaaccctc atgcctcgta tgtggcggga gatggcgggc tgattaccgg
     6001 ccctgacggg ctggtaattg cccggtttgc ctgggtaacc aaaggtgttg ccgctaataa
     6061 gggggccggc gcaccggcgg gttttgttcc tcgtgacggg caggcttcca ttgtggaatg
     6121 gctggccggg gactcgaaca ccatttatcc ggggcgtgaa tgcaccctga tggtgtcggg
     6181 ggatttctgg gcgctgacca ccactactgc gacgatcggg cagaaagtat ttgcctcact
     6241 ggccaccggg gagatagcca cgggggcggc tggcacctcg atggcggggt ttgtcgaaac
     6301 ctggttttcc gttgccagcg ctgcggcggc aaaagaagtc attaagatca gtacctggag
     6361 caaataatga ataaatttaa acagcattat gcgacggcaa gtcgcgacta cgggattatt
     6421 cttcccggag cacaggctta tttgccgaag gagtacgctg ccgattacgg actggcgatg
     6481 gacgcgcagc ctgcgctggt cacagcggct aacagcggta tccctgcgta tttcaccaat
     6541 tacgttgagc cagaactgat ccgcgtgctg gtgacgccga tgaaagccgc tcagattctg
     6601 ggcgaaacca aaaaaggtga ctggacgaca ctgtcggcac aattcccgat tgcagaatct
     6661 gacggggagg tgagttccta cggggattac agcaacaacg gggtggtaag cgcaaacgtc
     6721 aactgggtac cgcgccagag ctatcacttc cagaccttca ccgtatgggg cgaacgtgag
     6781 ctggagatgt acggcgcagc ccgtattggc tgggcggcag agctgaacgt ggcgtcagca
     6841 ctgacgctga ataagttcca gaataagtcc tatttctacg gtattgccgg actgacgaac
     6901 tacggtttgc tgaatgatcc gtcgttatct gcaccaatca ccccggatac cgttgacggt
     6961 aaactgaagt gggacgacaa ggacggacag ggcgtgtatg acgatatcgt gaaactcttt
     7021 aagcaactgg tgaaacagac taacggtcat attgagcgta ccgacaaaat gaaactgtgc
     7081 atgtcgccgc tggcagaggt gaacctcacc aaaacaaacc agtacagggt taacgtgtcc
     7141 gatctgctgg cgaaaaattt cccggcgatg accattgaaa cggcggtgga atacacctct
     7201 gacgctggcg agctggtaca gcttatcgcg gagcgtctgg gtgaacagga tacaggctat
     7261 tgcgctttca ctgaaaaaat gcgcgcccat gcggtagtgg cagatacgtc ttcctggaaa
     7321 cagaaaaaat ccgctggtac ctggggggcg attattcgcc agccgctggc gtatgcacaa
     7381 atgctggggg tgtgagttat ggctgaaatg gtaacagtgg gctgcaaact gccgaacggt
     7441 ctggtgctgg aagtgggtca gaaacgggtt caggtggcag gctggcggaa taacgcggtt
     7501 aaaattgtgg ggggctatgg tctgacgcag gtcgataagg tgttttttga cgcctggctg
     7561 gcggagcacg gacagcaacc ttatgtgaaa aatggcgtta tttttgcgca ggataaggcg
     7621 aacagtgcag tctcgcaggc tacggagcag aaagccgtta aatccggcct tgaaccgctg
     7681 ccgcagaaaa atccggctcc gggcgttaac cgcaatgatg aagtgatggg taaacctcag
     7741 gagtaaacgc tatgggtacg gtgacgtttg actgtcggat gtttgtcagt ctgtacccgg
     7801 agttttccat ggtgacaccg gagctactga cggcggtgtt taaccaggcg accgcgttgt
     7861 acctggataa cacggacgac agtccggtta ccgacctgaa ggagcgggaa cagcttctgt
     7921 tcctgctggt tgcgcatctg tgcgcattgc gggggctggg aaacgggaga gacgggcagg
     7981 ctggtctggt ggggcgtatc accagtgcgt cggaaggttc tgtatcggta tcggtggata
     8041 atagcggcag taatgatgcg tcgtggtggt atctccagac cccctggggt gctgactact
     8101 ggcaggcgac agcgccatac cgctcaatgc agtatcattc cgggggggca ccttcgcgat
     8161 atccggggca ttattaccgg ggatacggga ggggcgtcga tggtaaataa agtcacaggt
     8221 ggcaaacagt tccggcagaa gctgaaacag gttgccgcga acctttcatt gggtaaaaaa
     8281 ctcaaagtgg gttttcttga gggagcaact taccctgacg gtacgtcagt ggcttatatt
     8341 gccgcagttc aggagtttgg tggaagagcg gtcatacccg cccgtgagca gacgctccac
     8401 tttcgctata acgaaaaaac gggagaaacc gggcaccgct ttgtcaaagc cggtaaaggg
     8461 aattttgttc aggatgtggt tattcctgag cacacggtca ccattccccc ccgtccgttc
     8521 ttccgtaaga tgatcgaaca taaaagtccc gaatggggcg aaaaaatggc gaccttatta
     8581 cgggcgaatg attttgatac tgcgaccgct ctggtgtgta tgggagaaca tatcaaaggg
     8641 cagttgcaga tgtctgttcg cgactgggaa agtccgccca acgctgcatc cactgcccgg
     8701 caaaagggtt ttaacaaccc gcttattgaa accggacata tgatggacag cgttgattat
     8761 tctgtagacg ggggcaaaaa atgaactttc acggtattgt ttccggcgca gtacgtcggg
     8821 taaatccctt tacagacgcg ctggtttatc gttcccgcgg gagtacacag caggcagatt
     8881 attcccgcgt acctgagtat gaagatccgg tttccgtcag ggtgcaaaag caggctgtca
     8941 cccaggctga tttacgtcat cttgataatc ttaaccagca gggagctttc gccacattgt
     9001 ataccgatgg taactggtgc gggcttaacc gtgcccggca acagggaggc gataaatttg
     9061 ttattggcga tgaaacgtgg ctggtgattg aggtaccgga aatctggcct gactggacga
     9121 gggttattgt atgccttcag gtgtgaccct ctccgttacg gaaagcgctc tttatcaggc
     9181 cctcggtgat tatctccggg ggcttttttc tgatgctggg attgaacgaa cgcagcagaa
     9241 ccaggtcccg atgcctcagg ggaatttcat caccatgacg ggcattgatg ttaccggatt
     9301 atccactgcg gtagtggcat attctgcgcc agcgcaggct ggtgagggct ctcagcatat
     9361 cacccgtacc acaaaatggc gttgccagct tgatttctac ggccctcacg cggcagataa
     9421 cgcgcaggtg ctggcaacgc ttttccggtc tgaattttcc gtacggcttt tccggcaggg
     9481 cggtgggctg atttccccgc tgtattgctc agagcccttt aacaccacat tccccaacgg
     9541 tcagcaacag tatgaaccgc gctggacgct tgatattcag atgcagatta accctgtggt
     9601 cacaacttcc ctgatgtttt ttgacaacgt gatcacccgg acaacggagg ctgataatgc
     9661 caattccact cagtaaagat gtacagataa atcccggtgt gctggctgtg gcgggtaatg
     9721 ccgttgatct taacggcctg ctgttgacca gtaatccgtt acttccggtc ggcactgtgg
     9781 ttccgttttc ctctccggat gatgtggccg cgtattttgg tgcattatcc gatgagtacg
     9841 cacgcgctca gctttatttc cagggcttca aaaatgccac caggacgccg gggcaattgt
     9901 tgttttcccg tttcagtcct gccccatcgg cggcctggtt acgtagcggt tcgtttaagg
     9961 gcgcgactgt tgaacagcta caaaaacttt ccggcacgct gatgctgagt attaacggga
    10021 aaagcgtcag cattgaagtg aattttaacg gtgtcgccag cttcgccgct gctgcaacgg
    10081 cactacagac agcgctgacc gcagcagtgg ctacagtggt attcgatacc acacagaatg
    10141 cttttgtcat tactgtcgcc ggggcgaaac ccgggagtac cacgataacg ttcggcagtg
    10201 gatcggcagc agaactcctg aagatgacca gtaacaccgg tgcggtgata tcccggggcg
    10261 cgcctgtatc tgatgtccct gacacaatgg cagccattaa ggccgcttcc cagcaatggg
    10321 cgggtttttc cacggtatct gaggtcactg acgagcaaca cctggcgttt tctgcatggg
    10381 caaacgggca gggcaagcgt tacttttatg tggcatggac aaccagcggc aacgccagag
    10441 taaaaggcag tactgaa
//
LOCUS       DADLSA010000132        10415 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.132, whole genome shotgun sequence.
ACCESSION   DADLSA010000132 DADLSA010000000
VERSION     DADLSA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.132"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="JN691_003248"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="JN691_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAY7244615.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="JN691_003249"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="JN691_003249"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAY7244616.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPPFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="JN691_003250"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="JN691_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HAY7244617.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGVGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="JN691_003251"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="JN691_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY7244618.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="JN691_003252"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="JN691_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="JN691_003253"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="JN691_003253"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY7244619.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="JN691_003254"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="JN691_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAY7244620.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9370)
                     /locus_tag="JN691_003255"
                     /pseudo
     CDS             complement(8007..9370)
                     /locus_tag="JN691_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(9393..10085)
                     /locus_tag="JN691_003256"
     CDS             complement(9393..10085)
                     /locus_tag="JN691_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAY7244621.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLMAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2553 a         2515 c         2753 g         2594 t
ORIGIN      
        1 aagggggggg ctttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctact aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg gtgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgctg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgttgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccactgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtac tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag cgggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgccagacgc ctcctttctt cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaagct gcggatcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccaaggtgta cctcctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg cagcgttaaa tgtctatccg ggccgtgtga
     2701 tggcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct
     3001 ttaaaagcga caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 caggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgta ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgct gatgaacagc
     3721 aggctgtgag tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt attgatcctt gatgagccta cccgtggagt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagtatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cttcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatag acttgtcggt aggctctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcac gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgtccaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggctgggat cattgaagtg gcgcgtctct cctctgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataaacttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt gggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacattccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 ggtgaagtgg tgagccacat tgcatctgat aacgtactgg gcggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgccggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcaccaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgttg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtactggc gatgacagca ttggcgagag cgttcgccag cagctcgcca ctgataatat
     6361 cgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggcgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ctctttcccc
     6481 atcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcgttat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaag ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc agccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgtttggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagctctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttactgtt actgtgtacc gaaacgcatc agccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc taccgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgctgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggcata
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagatc agcatccata tcgccattgc cggtgattgc aaagagaact
     8341 gagcgatcat tagcccaata atcaccgtga tccccactaa tgtatggcga tagcccagac
     8401 gacgtaagac ttgggtaacc atcgattttg caataatgga acctaacgct gtcggtgcca
     8461 tcatacagcc ggcaataaac gcctgataac caaatcctac ctgtaacatc aatggcataa
     8521 ggaacggtac acagccggtc cccagacggg ttgcaatatt gcctacgata ccgatcgaga
     8581 aagtgcgggt tttaaataaa tctaatgaaa ttaatgggtt tggcgtacgt cgcgcatgga
     8641 gaatatagag aagcagtaac ccgatgctgg tgacaattac cgtcaaggaa atccagctgg
     8701 cgacaatctt ttccccgaat agctctattc cgcttgaaaa gagaacaagg ctgaggccaa
     8761 acagcaaaaa gccagtgata tcgaatctgc gtcgtgcggt ggtgaaattg ggcatatgtt
     8821 tgcgcgcgta aagaaggccc gcaataccta tggggatatt aattaaaaat atccagtgcc
     8881 aggttgccca ggtgaccagc acgccgccaa gaacggggcc taaaattggc cccaccagac
     8941 ccggcatggc gacaaaattc aataccggaa gaagttcatt acgaggataa gcacgcagta
     9001 aggccagccg agcaacaggc atcatcattg cgccgcctat cccttgaata acccggaaga
     9061 caaccagctg tggtagcgaa ttagaaagtg cgcaggccag agaacccaac gtgaacagac
     9121 tcacggcaag ggtaaaaatg cgacgcgtac cgaagcgatc ggctagccat ccgcttaccg
     9181 gaataagcat cgccaccgtc agcgtataac tgatgatggc tgattgcatc gcgagaggag
     9241 aacgattaag gctatgagcg attgcgggta aggcggtatt cagaatagtg gcatcaagtg
     9301 cctgcatgaa gaaggccatc gccgcgatcc acggcaaacc cgacatacta cgcttctttt
     9361 tatcgctcat tcaatgtcct gttatcgggt tatcacttat caggtgagcg tagcagcgcc
     9421 tgacaagctt taaatgccgc gtcgccatcg ctttggataa tcgcatcgac aatcgcctgg
     9481 tgcagatcca gctttatcac tgtgtcgctg gtaattgacg tgaagtaagt gtgataaacc
     9541 gaatggaata gcgaggcgaa tgaggtcaaa aacggattgg cgctcatttc atagatatgc
     9601 tcatgccagg ccatatcgac ttcgatccag cgttcacggc gaaagttctc ttttaatgcc
     9661 gccatttcgg ccattaacgt attgagatgc gccttctgtt ccgcggtgcc aaccgttgct
     9721 gccagtaggc aggcttgcgg ctccagacag atacgcataa ccagaaagtg atcgatgacc
     9781 tgatgaaagt tctcttctgt catccaccag gtaagcaatt cctgatcaag aaaattccag
     9841 ttcgattgtg gcatgacccg agtaccaatt cgcggtcgcg gtaaaaccat cccttttgcc
     9901 attaacgttt tgaccgcttc gcgtaccgct gtacgactca ctccaaattg ctcgcccagc
     9961 tcaatctcac cgggcaaaat ggtgccgggt tcatattcac cttttaagat ccgttgcgcc
    10021 agcttctcag ccagaacata cgaaaggttt ttctgtgcag ctaactgttg tgcgcttaaa
    10081 ggcattactt atcttccttt ttctttttat tcctccttag tatgccacca ggaagtgtga
    10141 ttacggttgc aaaaacggca aattgcttgt tttatggcac attaacgggg cttttgctga
    10201 aaaaatgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct
    10261 ataatgcgcc accactgaca cggaacaacg gcaaacacgc cgccggggca gcggggttct
    10321 cctgagaatt ccgacagaga aagcaaaaat aaatgcttga ctctgtggcg ggaaagcgta
    10381 ttatgcacac cccgcgccgc tgagaaaaag cgaag
//
LOCUS       DADLSA010000133        10405 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.133, whole genome shotgun sequence.
ACCESSION   DADLSA010000133 DADLSA010000000
VERSION     DADLSA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.133"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(38..364)
                     /locus_tag="JN691_003257"
                     /pseudo
     CDS             complement(38..364)
                     /locus_tag="JN691_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(374..922)
                     /locus_tag="JN691_003258"
     CDS             complement(374..922)
                     /locus_tag="JN691_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAY7244622.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(922..1629)
                     /locus_tag="JN691_003259"
     CDS             complement(922..1629)
                     /locus_tag="JN691_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY7244623.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAISAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIANGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1644..2321)
                     /gene="ydjY"
                     /locus_tag="JN691_003260"
     CDS             complement(1644..2321)
                     /gene="ydjY"
                     /locus_tag="JN691_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HAY7244624.1"
                     /translation="MSQHYSVSWEKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2326..3036)
                     /locus_tag="JN691_003261"
     CDS             complement(2326..3036)
                     /locus_tag="JN691_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY7244625.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3203..4009)
                     /gene="xthA"
                     /locus_tag="JN691_003262"
     CDS             complement(3203..4009)
                     /gene="xthA"
                     /locus_tag="JN691_003262"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAY7244626.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4455..5675
                     /gene="astC"
                     /locus_tag="JN691_003263"
                     /pseudo
     CDS             4455..5675
                     /gene="astC"
                     /locus_tag="JN691_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            5672..6706
                     /gene="astA"
                     /locus_tag="JN691_003264"
     CDS             5672..6706
                     /gene="astA"
                     /locus_tag="JN691_003264"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HAY7244627.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            6703..8181
                     /gene="astD"
                     /locus_tag="JN691_003265"
     CDS             6703..8181
                     /gene="astD"
                     /locus_tag="JN691_003265"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HAY7244628.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            8178..9521
                     /gene="astB"
                     /locus_tag="JN691_003266"
     CDS             8178..9521
                     /gene="astB"
                     /locus_tag="JN691_003266"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HAY7244629.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            9514..>10405
                     /gene="astE"
                     /locus_tag="JN691_003267"
     CDS             9514..>10405
                     /gene="astE"
                     /locus_tag="JN691_003267"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAY7244630.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHD"
BASE COUNT         2392 a         2659 c         2917 g         2437 t
ORIGIN      
        1 aaaaagagcc agtcagttgc ttaactgact ggcattactc ttttaaggtg cggaagtttt
       61 cgccgttcac ccagagcaga tccaccgagc cgcccgtttt acgaccggca gcagcttcgg
      121 tctgaatgcg cttcaccgcg tctgcggcat ccgcgagacg aacaatcttc aggtttatag
      181 cgtaatgggt tttcatctcg ccgctaaccc agtcgagata gcggttaatt gcggtatcgc
      241 cgccccaggc gttaaaccag acggtttgcc ctttggcctc atttttaatt tcctgccagt
      301 cactggcatg tgttgccaaa gaaaacagcg ataacaatcc cagcaaccac ccacaatggc
      361 gcatcttccc cccttaagaa cgtttttccg gaatacggca cagcccctga gcgggaatgt
      421 ccatggcaga attaaaacga gccgacaggt tttgcaggcc gatcaatgcc gtcagttcgg
      481 tgagcgcctg agcgtcaaaa tgcgcagcca gtcgggtacg cagggcatca tcgactgttg
      541 gcggtgtgac gcttgcggct tcggcgtact ccagcgccag ccgttcttca tcgctaaaga
      601 gcgggctttg ccgccagtca gccactgcca gcagtttatc ggtgctgccg gtacgctcgg
      661 cgactttcat actggtgata tccacgcaaa actcacacag gcacatttgc gcaatgcgcg
      721 cgctgacaag cgatcgcact accggatcga gcggtgagcg tttgcgctcc agccacccaa
      781 caaacattga caccaaataa aagataaacg ggatccgccc ccaccagcga atcgggctta
      841 gcacctcgcc gtaatggcga cgatgcagcc acgcctgcgg acgtaaaatg aaaggaattt
      901 tgctaagcgg cggtaaaccc atcagtcatt cctcttctgg cgctcaagcc atattttctt
      961 cgccataaaa atcaccacgg tcagggcaaa cagaataaac agtccggtga caaaccagaa
     1021 agtaccgcct gttaacatgc tgcccgccca ggaataaaca atagtcgccg gtaattgacc
     1081 aagcccgttg gcgataaaaa acgagcgaaa acgtattgaa gtcagaccgg cagcatagct
     1141 gattggatcg aaagggacaa aaggcaataa ccgacagacc aggatggtgt gtttgccgta
     1201 gcgagtgaaa aagccgtcca tactgtcaag cacggttttg ccggttaatt tttccaccac
     1261 ttcgcggccc atcactctgg cgataaaaaa gcacagcgcc gcgccggcca tcgaactggt
     1321 ccacgacagc aagccgcccc agaacgcgcc aaacagcgac gcattggcaa aggtgatcaa
     1381 aaacgcaggt agcggtgcag aaatggcctg taaaatcatc aacaagaacg agacaactgc
     1441 cgccagtgcg ccgtaagact gaataaagcg ttctataccc tgttggtcca cggcggcaaa
     1501 cgccgcaacg ctgcgattga taaactcatg gacgccggga agcagcgccc atgcgagcag
     1561 catagcgaac aacaggatga tgagggttat acggtagtac cagattttac gcgattgtat
     1621 cttcatcatc ctttatcctg gctttattcg gtgattttaa aggtaaccgt tgccagcgtg
     1681 ttatccgccg ggagaactga ggcattgcct ttgaatttca cttcgccgcg tttttccacc
     1741 gcaccataag tgtatgttgc attgctgacg atgccgaccg ggcagctatc cagacacacc
     1801 aggcaacccg ttttcttctc ttctgctgcc gttaaattac cgccaaagcg catgtccagt
     1861 ttcttgccat tactgtcaac aatcacttca tcgaaggaat acgcttttgc cgccccttgc
     1921 cagttgaccg aaatatccag tttgctgcct gtgacatgag tcgtttcttt attatccatc
     1981 gtcatgtttt cgcccggcgt accacctgcc tctttcaggg cttcataaaa tgctttcggg
     2041 gtcgcataag ccataaacag cgatttatgt ccgttggagc catctttaaa cacaataccg
     2101 tgacgagtgt cgtcggtgag gaaacgacca ttaatttgca ccagcatggt taccgtatgg
     2161 ttattggcat cgacgcgcag cggttggctg ttcgaaagtc cgtcgtattc cacttttgcc
     2221 gccgcattct ctttttgatc gcagccgcta agcccggcaa cagccagtaa gcagagtgca
     2281 gccagtcctt tttcccatga cactgaataa tgttgcgaca tcgttctatc cttcattttt
     2341 tgggtgagta agtgggctgc ggcgcgaagc agacagatcc acatgtttgt gtcgggcgta
     2401 gagttttgcg agctggatga gaataaacag cgccaggccc gccagacaga gttgtaaaat
     2461 aaagcgcagc gtaatgcctt cattggcgag atcgcttgcc atcacggtat aaataacaat
     2521 accgggcagg gtcgtgagtg ccgaaataag ggtataaggc cagaaggcga tggtggttaa
     2581 tccgtaagcg taattttgaa tattgtaagg aaacaacggg attaagcggg tcagaataag
     2641 aaaatcaata ccgttacgcg caatgccttt ttcaatggcc tggaaggtat ggctatggcc
     2701 cacgtatttc agcagtaaat cacgtcccag ccagcgcgcc agcaggaatg agcacgagga
     2761 ggccagcgtt gcggcaatta atgagagtag tgtccctaag agcgggccaa aaactattcc
     2821 tccagcgatc accagtatgc ttcctggtaa cagaaagagg gtggcattga tgaataacag
     2881 aatatagagg ctataaccga acaatccgct ctggcgaatg agtgtctgta agtggggtaa
     2941 atcggtgagc agatcgaata aaccgaaagc gtggatagcg taaatgacca gcgcaaaaat
     3001 aagacaggca aacagaaagt tacgctcagc gttcatcgtg gagtttccta tatcaaactt
     3061 gataatagag aagtggtcag acaactaaaa acgcagtgac ggtataacaa aggacggcag
     3121 gcaacaaatc attactgaca gtttgttgat ttttctcaag gttaattctc ctgacccagt
     3181 ttgagccagg agagctgcta aattagcggc ggaaggtcgc ccagacgggg gcgtgatcgg
     3241 acggtttttc catgctgcga atttcatagt cgatgccggt ttctacgcaa cattctgcca
     3301 gcggttggct ggcgagcagc aggtcgatgc gcagaccacg gttatcgtca aaaccttttg
     3361 agcggtaatc aaaccatgag aaacgatctg ctgtttgcgg attagcatgg cggaaggtat
     3421 cgaccacccc ccagctcatc agcctgtcca tccattcgcg ctcttccggc aggaaagagc
     3481 atttaccggt acgcagccag cgcttacggt tttcttcgcc aatgccgata tccagatctg
     3541 tagggctgat attcatatcg cccataatca gtaccggatt atcacgtttg agttcggttt
     3601 ccaggtagtt ttgcagattc tgataaaact gcgcttttgc cgggaatttt atcggatggt
     3661 cgcggctttc accctgcggg aagtaaccgt tgatcacggt gacattaccc agcagtgagg
     3721 ggatttccgc cataataatc cgccgctgcg cctcttcgtc gtcaccggga aagccgcgac
     3781 gcacggcaat cggcgtctct ttggtcagca gcgccacgcc ataatggcct ttctgcccgt
     3841 gataaaacac gttgtagccg agcttcgcca cctcttcgag cggaaacata tcgtcatgaa
     3901 cttttgtctc ctgcaggcca atcacatccg gttggtgctt ttcgacgatg gcttcaagct
     3961 ggtgaggtct ggcgcgcagg ccgttgatat taaaagagac aaatttcata gtcgctgcca
     4021 tttattcaga taaagagtgc gtggatggta gcagaatttc gcgttgctta ccgcctgttg
     4081 ttaagtgatt tgatcgatgg ttgttaatga tgtcaacgat ggcgcaaaaa atgcccgctt
     4141 ttggtgcgcg ctgcgtcaga atggcgcagt aatttccagt aaatttcgtc aatgatcaca
     4201 attccagaat tatattttac ttctgcataa cattgcgttt tttattcttt ttattcaccc
     4261 acatcgcaaa cgtattcact ttatatgcac tttaaatgca tatggtttgg ttataactcc
     4321 ttgatttcca gttagcctcc gccgtttatg cacttttatc actggctggc acgaaccctg
     4381 caatctacat ttacagcgca aacattactt attgttaaca tacaaataac gaattattta
     4441 ctgtagaggt cgctatgtct cagccaatta cgcgtgaaaa ctttgatgaa tggatgatac
     4501 ctgtttacgc tccggcaccc tttataccgg tacgtggcga aggttcgcgc ttgtgggatc
     4561 agcaggggaa agagtatatc gacttcgcgg gtggcattgc ggtgaacgcg ctgggccatg
     4621 cgcatccgga actgcgtgaa gcgctgaacg aacaggcgag taagttctgg cataccggca
     4681 acggttacac caacgagccg gtactgcgac tggcgaaaaa attgatcgac gccacgtttg
     4741 ccgatcgcgt cttcttttgt aactccggtg cggaagccaa cgaagcggcg ctaaaactgg
     4801 cgcgtaaatt cgctcacgac cgctacggca gccataagag tggcatcgtg gcgttcaaaa
     4861 atgcgtttca tggtcgcacg ctgtttactg tcagtgcggg tgggcagcca gcctattcac
     4921 aggattttgc gccactgccg gcggatattc gtcatgctgc atataacgat attaactctg
     4981 ccagcgcgct gattgacgac gccacctgtg cggtgattgt cgagcccatc tagggcgaag
     5041 gcggtgtggt gccagccagc aacgcgtttt tacaaggtct gcgtgaattg tgtgaccgcc
     5101 acaatgcctt gctgattttt gatgaggtac aaaccggcgt cgggcgcact ggtgagctgt
     5161 atgcctatat gcactacggc gtgacgcccg atctgttaac caccgccaaa gcgctgggcg
     5221 gcggtttccc ggtcggtgcg ttgttggcaa ccgaagagtg cgcccgcgtg atgaccgttg
     5281 gcactcatgg caccacctat ggcggtaatc cgctggcctc ggcggtggca ggcaaagtgc
     5341 tggagctcat caacacacca gagatgctta atggcgttaa acagcgtcac gactggtttg
     5401 ttgagcgtct taatactatt aatcaccgct atggtttgtt cagtgaagtt cgcggcttag
     5461 gtttgttgat tggctgtgta ctgaatgccg attacgccgg gcaagcgaaa cagatctctc
     5521 tggaagcggc gaaagcaggc gtgatggtac tgattgcggg tggcaacgtg gtgcgttttg
     5581 cgcctgcgct caatgtcagc gaagaagagg tgacgaccgg actggatcgc tttgcagctg
     5641 cttgcgaaca ctttgttagc cgaggttcat catgatggtc atccgtcccg ttgagcgctc
     5701 agatgtctcg gcgctgatgc agcttgccag caaaacgggc ggcggcctga cgtcgcttcc
     5761 cgccaatgaa gccacgcttt cggcgcgtat cgaaagggca atcaaaacct ggcaaggcga
     5821 actgcccaaa agcgagcagg gctatgtgtt cgtgctggaa gatagcgaga caggcaccgt
     5881 ggcggggatt tgcgctattg aggtggcggt tgggctgaac gatccctggt acaactatcg
     5941 cgtcggcacg ttggttcacg cctcaaaaga gctgaatgtc tataacgcat tgccgacgct
     6001 gtttcttagt aacgatcaca ccggcagcag cgagctgtgc acgctgtttc tcgacccgga
     6061 ctggcgtaaa gagggcaacg gctatttgct gtcgaaatcg cgctttatgt ttatggcggc
     6121 ttttcgcgac aagtttaatg acaaagtggt tgctgaaatg cgcggggtga ttgacgaaca
     6181 cggctattca ccgttctggc aaagcctcgg taaacgcttc ttttcgatgg attttagccg
     6241 cgccgatttt ctctgcggca ccgggcaaaa ggcatttatt gcagaactga tgccgaaaca
     6301 tccgatctat acccactttt tatcccagga agcccaggac gtcatcggtc aggtacatcc
     6361 gcaaaccgcg cctgcccgcg cggtgctgga gaaagaaggt tttcgttacc gcaactatat
     6421 cgacatcttc gacggcgggc caacgcttga gtgtgacatc gaccgcgtgc gcgccatccg
     6481 taaaagtcgg ctggtggaag tggcagaagg gcagcctgcg cagggcgatt ttccggcctg
     6541 cctggtcgcc aatgaaaatt atcaccattt ccgcgtggtg ctggcgcgta ccgatccggc
     6601 aaccgagcgt ttgattttaa ccgccgcaca actggatgcc ctcaaatgcc acgcagggga
     6661 tcgcgttcgt ctggtgcgcc tgtgcgcaga ggagaaaaca gcatgacttt atggattaac
     6721 ggtgactgga taacgggcca gggcgcatcg cgtgtgaagc gtaatccggt atcgggcgag
     6781 gtgttatggc aaggcaatga tgccgatgcc gctcaggtcg aacaggcttg tcgggcagcc
     6841 cgtgcggcgt ttccgcgctg ggcgcggctc tcattggctg aacgtcaggt cgttgtcgaa
     6901 cgctttgccg gattgctgga aagcaataaa gccgaattaa ccgcgattat tgccagagaa
     6961 acgggtaagc cgcgctggga agcggcaacc gaagtgacgg cgatgatcaa taaaatcgcg
     7021 atatcaatta aggcgtatca cgttcgtacc ggcgagcagc gtagtgaaat gccggacggt
     7081 gcggcgagcc tgcgacatcg cccgcacggc gtgctggcgg tgtttgggcc gtataatttc
     7141 cctggtcatt tgccgaacgg acatatcgtt ccggcattgc tggcaggtaa caccattatc
     7201 tttaaaccca gcgaactgac accgtggagt ggcgaagcgg taatgcgttt atggcagcag
     7261 gctggcttgc cgccgggcgt gctgaacctg gtgcagggcg ggcgtgaaac gggtcaggcg
     7321 ctgagtgcgc tggaggatct cgacggtttg ctgtttaccg gtagcgccaa taccggctac
     7381 cagctgcatc gccagctctc cggtcagccg gagaaaattc tcgcccttga gatgggcggt
     7441 aataatccgc taattatcga tgaggtggcg gatatcgacg cggctgtcca tctgaccatt
     7501 cagtcggcgt ttgtcacagc cgggcaacgc tgcacctgcg cccgccgttt attgctcaaa
     7561 agcggagcgc agggcgatgc gtttcttgct cgtctggttg ccgtcagcca gcgattaacg
     7621 ccgggcaact gggatgacga accgcagccg tttattggcg ggctgatttc tgaacaggcc
     7681 gcacagcagg tggttactgc ctggcagcaa ctggaagcga tgggcggacg aaccctgctt
     7741 gcgccgcgct tattacaagc agggacatcg ttgctgacac cagggatcat tgaaatgacg
     7801 ggcgttggcg gtgtgccgga tgaagaggtg ttcgggccgc ttttgcgcgt ctggcgttat
     7861 gatacttttg atgaggcgat tcggatggcg aacaacactc gcttcggact cttttgcggt
     7921 ctggtttccc ccgagcggga aaaattcgat caactgttgc tggaggcgcg ggcggggatt
     7981 gttaactgga acaaaccgct tactggtgct gccagtaccg cgccattcgg cggcattggt
     8041 gcttccggta accatcgccc cagcgcctgg tatgccgcag attactgcgc atggcctatg
     8101 gcgagcctgg agtcggactc gttaacattg cccgccacgc ttaaccccgg gctggatttt
     8161 tccgatgagg tggtgcgatg aacgcctggg aagttaattt cgacgggctg gtagggctga
     8221 cgcatcatta cgcgggcctg tcgtttggta atgaagcctc tacccgtcac cgttttcagg
     8281 tgtctaaccc gcgactggcg gcaaaacagg gcttactgaa aatgaaagcc cttgccgatg
     8341 cgggattccc ccaggccgtg atcccgccgc acgagcgtcc gtttattccg gtgctgcgtc
     8401 agttgggatt cagtggtagc gatgagcagg tactggaaaa agttgcacgc caggcaccgc
     8461 actggctttc cagcgtcagt tccgcttcgc caatgtgggt agccaatgcg gcaacgatcg
     8521 cgccatctgc cgatacgctg gatggcaaag tgcatctcac cgttgccaac ctgaacaata
     8581 aatttcaccg ttcgctggaa gcgcccgtca ctgaatcgct gttaaaagcg atttttaagg
     8641 acgaagagaa atttagcgtc cattcggcgt tgccgcaggt agcgttgctc ggtgatgagg
     8701 gggcggcaaa ccacaatcgt ctcggcggtc attacggtga accgggtatg ctactttttg
     8761 tctacgggcg agaagaaggc aatgataccc ggccttcccg ttatccggcg cgacagactc
     8821 gcgaagccag cgaggcggtg gcaaggctta atcaggtgaa tccccaacag gtgattttcg
     8881 cccagcaaaa cccggacgtt atcgaccagg gcgtttttca taatgacgtg attgccgtga
     8941 gtaaccgcca ggtgctgttt tgccaccaac aggcgttcgc tcgccagtca cagttactgg
     9001 caaacctgcg ggcgcgggtg aacggtttta tggcgataga agttccggca actcaggttt
     9061 ccgtgtcgga tgcggtgtct acctatctgt ttaacagcca actgttgagc cgcgatgatg
     9121 gttccatgat gttggtgctg cctcaggagt gtcgggaaca cgccggagta tggggttatc
     9181 tcaatgaact ccttgccgct gacaacccga ttagcgaact aaaagtcttt gacttacgtg
     9241 aaagcatggc gaatggcggc ggcccggcgt gcctgcggtt gcgggtggta ttgacagaag
     9301 aagaacgccg ggcggtgaat ccggcggtga tgatgaacga taccttgttt aatgcgctca
     9361 atgactgggt ggatcgttac taccgcgatc gtcttactgc tgccgatctg gccgacccgc
     9421 aattgctgcg cgaagggcgg gaagcactgg atgtattgag ccaattactg aatctcggtt
     9481 cggtttatcc gttccagcgc gagggagggg gcaatggata attttcttgc tctgacctta
     9541 acgggtaaaa aaccggttat caccgagcga gaaatcaacg gcgttcgctg gcgctggctg
     9601 ggcgatggtg tgctggaact gacgccatta acgccaccgc aaggcgcgct ggtgatttct
     9661 gcggggatcc acggtaatga gacggcacct gtggaaatgc tggacgcgtt gcttggcgcg
     9721 atatctcacg gcgagatccc gttacgttgg cggttgctgg tgatcctcgg gaatcctcct
     9781 gcgctgaagc aagggaaacg ttattgccat agcgatatga accgaatgtt tggcggtcgt
     9841 tggcagctat ttgctgaaag cggagaaacc tgtcgggcgc gcgaactgga acagtgcctg
     9901 gaagattttt atgaccaggg caaagaatct gtgcgttggc accttgatct acataccgca
     9961 attcgtggct ccttgcatcc gcagttcggt gtattaccgc aacgcgacat tccctgggac
    10021 gagaaatttc tgacgtggct gggtgcggcg gggctggagg cgctggtgtt ccatcaggag
    10081 cctggtggta cgtttaccca tttcagcgtc agacattttg gcgcgctggc ctgtacgttg
    10141 gaacttggca aagcgttgcc ctttgggcaa aacgatcttc gccagtttgc agtaactgcc
    10201 agcgcaattg ctgcgctgct atctggtgag agtgtcggta tcgtgagaac accgccgctc
    10261 cgttatcggg tggtttcgca aattactcgc cactcgccgt ccttcgaaat gcatatggca
    10321 agtgacacgc tgaattttat gccgtttgag aaaggaacat tgctggcgca ggacggagag
    10381 gaacgtttta ccgtaaccca tgatg
//
LOCUS       DADLSA010000134        10137 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.134, whole genome shotgun sequence.
ACCESSION   DADLSA010000134 DADLSA010000000
VERSION     DADLSA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.134"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JN691_003268"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JN691_003268"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAY7244631.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JN691_003269"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JN691_003269"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAY7244632.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3325)
                     /locus_tag="JN691_003270"
                     /pseudo
     CDS             complement(2698..3325)
                     /locus_tag="JN691_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3455..5215
                     /locus_tag="JN691_003271"
     CDS             3455..5215
                     /locus_tag="JN691_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HAY7244633.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYGKLQRAKAAFWAK"
     gene            5621..6478
                     /gene="focA"
                     /locus_tag="JN691_003272"
     CDS             5621..6478
                     /gene="focA"
                     /locus_tag="JN691_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAY7244634.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6533..8815
                     /gene="pflB"
                     /locus_tag="JN691_003273"
     CDS             6533..8815
                     /gene="pflB"
                     /locus_tag="JN691_003273"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY7244635.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9007..9747
                     /gene="pflA"
                     /locus_tag="JN691_003274"
     CDS             9007..9747
                     /gene="pflA"
                     /locus_tag="JN691_003274"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAY7244636.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9829..>10137)
                     /locus_tag="JN691_003275"
     CDS             complement(9829..>10137)
                     /locus_tag="JN691_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244637.1"
                     /translation="LLKKRTNGSVRYCQYTSYGDGHKLPFNKVRWVALVGGDKESFVQ
                     MGWEKNISDYLKNMCSYLGIEDADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL
                     "
BASE COUNT         2599 a         2540 c         2404 g         2594 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacagac
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatcac attaccaggc
     3061 ggtataacaa cgccagagtg ataaagacga taagtaccgg caccatcggc acatcatcat
     3121 aaaacgccac atctcccgcc gctgaaccca gcgtgagaat gattaacact tcaaacagcg
     3181 acatctgtcg cacaccgcgt cttccggtca ttttgaggaa caaaaaaacc agtacaaagg
     3241 tataaagact acgtagtgca acttcgccaa ggaaatcaaa aggcacttta tcaaatgcca
     3301 tacggtggag atcgaacgcc ttcattttta tgctcttaac gtggtttatc cgctaaaagc
     3361 atagtcaaca gttcatcggc atggcggcgg tgatagcgcc tttgagataa aacccgtatt
     3421 attgcgcgct ttccgtacga ctaaagtgat tttcatgacg caaacattta tccccggcaa
     3481 agatgccgct ctggaagatt ccatcgctcg cttccagcaa aaactttcag acctcggctt
     3541 tcagattgaa gaggcctcct ggctgaatcc cgtgcctaac gtctggtctg tacatattcg
     3601 cgacaaagag tgcgcactgt gttttaccaa cggtaaaggc gcaaccaaga aagcggcgct
     3661 ggcttctgca ctcggtgaat atttcgagcg tctctcaacc aactactttt ttgctgattt
     3721 ctggctgggc gaaaccatcg ccaacggtcc atttgtgcat tatcccaacg aaaaatggtt
     3781 cccactgagc gaaaatgacg atgtgccaga agggctgctc gatgaccgtc tgcgcgcatt
     3841 ttacgatccg gagaatgaat tgaccggcag tatgctgatt gacctacaat ccggtaacga
     3901 agatcgtggt atttgcggtc tgccgtttac gcgccagtcc gacaatcaga ccgtttatat
     3961 tccgatgaat atcattggta acctgtacgt ttctaacggt atgtccgctg gcaatacccg
     4021 caacgaagca cgcgttcagg ggttgtccga agttttcgaa cgctacgtga aaaaccgcat
     4081 tattgctgaa agcatcagct tgccggaaat cccggcagac gtgctggcgc gttacccagc
     4141 ggtggttgaa gcgatcgaaa cactggaaac agaaggtttc ccgatcttcg catatgatgg
     4201 ttcccttggc ggccagtatc cggtgatttg cgtggtactg tttaatcctg caaacggcac
     4261 ctgctttgcc tctttcggtg cgcatcctga ttttggcgta gcactggaac gtaccgtgac
     4321 cgagctgctg caaggtcgtg gcctgaaaga tttggatgtg tttactccgc caaccttcga
     4381 tgatgaagaa gtcgctgaac ataccaacct cgaaacgcac tttatcgatt ccagcggttt
     4441 aatctcctgg gacctgttca agcaggatgc cgattatccg tttgtggact ggaatttctc
     4501 cggcaccacg gaagaagagt ttgctacgct gatggctatc ttcaacaaag aagataaaga
     4561 agtttatatt gccgattacg agcatctggg cgtttatgct tgccgtatta tcgtgcctgg
     4621 catgtccgat atttatccgg ctgaagatct gtggctcgcg aataacagta tgggcagcca
     4681 tttacgtgaa acgattcttt cgctaccagg cagcgagtgg gaaaaagaag attacctgaa
     4741 cctcatcgag caactggatg aagaaggttt tgatgacttt acccgcgtgc gtgagctgtt
     4801 gggtctggcg accgggtcgg ataacggttg gtacaccctg cgtatcggtg aattaaaagc
     4861 catgctggcg ctggctggtg gcgatctgga acaggctctg gtctggaccg aatggacgat
     4921 ggagtttaac tcatcagtat ttagtccgga acgcgccaac tattatcgct gcctgcaaac
     4981 gttgttatta ctggcacagg aagaagatcg ccagccgctg caatatctga atgcgtttgt
     5041 tcgcatgtac ggcgcagatg ccgtagaagc cgccagtgcg gcaatgagcg gcgaagcggc
     5101 gttttacggc ctgcaaccgg tagatagcga tctgcacgcg tttgctgcac atcagtcgtt
     5161 gttgaaggcc tacggaaagc tgcagcgcgc caaagcagca ttctgggcaa aataaaatca
     5221 aatagcctac gcaatgtagg cttaatgatt agtctgagtt atattacggg gcgttttttt
     5281 aatgccccgc tttacatata tttgcattaa taaaataatt gtaattataa ggttaaatat
     5341 cggtaatttg tatttaataa atacgatcga tattgttact ttattcgcct gatgctccct
     5401 tttaattaac tgttttagcg gaggatgcgg aaaaaattca actcatttgt taatttttaa
     5461 aatttatttt tatttggata atcaaatatt tactccgtat ttgcataaag accatgcgag
     5521 ttacgggcct ataagccagg cgagatatga tctatatcaa tttctcatct ataatgcttt
     5581 gttagtatct cgtcgccgac ttaataaaga gagagttagt gtgaaagctg acaacccttt
     5641 tgatctttta cttcctgctg caatggccaa agtggccgaa gaggcgggtg tctataaagc
     5701 aacgaaacat ccgcttaaga ctttctatct ggcgattacc gccggtgttt tcatctcaat
     5761 cgcattcgtc ttctatatca cagcaaccac tggcacaggc acaatgccct tcggcatggc
     5821 aaaactggtt ggcggcattt gcttctctct ggggctgatt ctttgtgttg tctgcggagc
     5881 cgatctcttt acttccacag tgttgattgt tgttgctaag gcgagtgggc gcatcacctg
     5941 gggtcagttg gctaaaaact ggctaaatgt ctattttggc aacctggtcg gcgcactgct
     6001 gtttgtactt ttaatgtggc tttccggcga gtatatgacc gcaaatggtc aatggggact
     6061 aaacgtccta caaaccgccg accacaaagt gcaccatact tttattgagg ccgtctgtct
     6121 tggtatcctg gcaaacctga tggtatgtct ggcagtatgg atgagttatt ctggccgcag
     6181 cctgatggac aaagcgttca ttatggtgct gccggtcgcg atgtttgttg ccagcggttt
     6241 tgagcacagt atcgcaaaca tgtttatgat cccgatgggt attgtaatcc gcgacttcgc
     6301 atctccggaa ttctggaccg ctgtcggttc tgcaccggaa aatttttctc acctgaccgt
     6361 gatgaacttc atcactgata acctgatccc ggttacgatc ggtaatatta tcggcggtgg
     6421 tttgttggtt gggttgacat actgggtcat ttacctgcgt gaaaacgatc accattaatg
     6481 gttgtcgaag tacgcagtaa ataaaaaatc cacttaagaa ggtaggtgtt acatgtccga
     6541 gcttaatgaa aagttagcca cagcctggga aggttttacc aaaggtgact ggcagaatga
     6601 agtaaacgtc cgtgacttca ttcagaaaaa ctacactccg tacgagggtg acgagtcctt
     6661 cctggctggc gctactgaag cgaccaccac cctgtgggac aaagtaatgg aaggcgttaa
     6721 actggaaaac cgcactcacg cgccagttga ttttgacacc gctgttgctt ccaccatcac
     6781 ctctcacgac gctggctaca tcaataaagc gttggaaaaa gttgttggtc tgcagactga
     6841 agctccgctg aaacgtgctc ttatcccgtt cggtggtatc aaaatgatcg aaggttcctg
     6901 caaagcgtac aaccgcgaac tggacccgat gatcaaaaaa atcttcactg aataccgtaa
     6961 aactcacaac cagggcgtgt tcgacgttta cactccggac atcctgcgtt gccgtaaatc
     7021 cggtgttctg accggtctgc cagatgctta tggccgtggc cgtatcatcg gtgactaccg
     7081 tcgcgttgcg ctgtacggta tcgactacct gatgaaagac aaatacgctc agttcacctc
     7141 tctgcaggct gatctggaaa acggcgtaaa cctggaacag actatccgtc tgcgcgaaga
     7201 aatcgctgaa cagcaccgcg ctctgggtca gatgaaagaa atggctgcga aatacggcta
     7261 cgacatctct ggtccggcta ccaacgctca ggaagctatc cagtggactt acttcggcta
     7321 cctggctgct gttaagtctc agaacggtgc tgcaatgtcc ttcggtcgta cctccacctt
     7381 cctggatgtg tacatcgaac gtgacctgaa agctggcaag atcaccgaac aagaagcgca
     7441 ggaaatggtt gaccacctgg tcatgaaact gcgtatggtt cgcttcctgc gtactccgga
     7501 atacgatgaa ctgttctctg gcgacccaat ctgggcaacc gaatctatcg gtggtatggg
     7561 cctcgatggt cgtaccctgg ttaccaaaaa cagcttccgt ttcctgaaca ccctgtacac
     7621 tatggggccg tctccggaac cgaacatgac cattctgtgg tctgaaaaac tgccgctgaa
     7681 cttcaagaaa ttcgccgcta aagtgtccat cgacacctct tctctgcagt atgagaacga
     7741 tgacctgatg cgtccggact tcaacaacga tgactacgct atcgcttgct gcgtaagccc
     7801 gatgatcgtt ggtaaacaaa tgcagttctt cggtgcgcgt gcaaacctgg cgaaaaccat
     7861 gctgtacgca atcaacggcg gcgttgacga aaaactgaaa atgcaggttg gtccgaagtc
     7921 tgaaccgatc aaaggcgatc tcctgagcta cgatgaagtg atggagcgca tggatcactt
     7981 catggactgg ctggctaaac agtacatcac tgcactgaac atcatccact acatgcacga
     8041 caagtacagc tacgaagcct ctctgatggc gctgcacgac cgtgacgtta tccgcaccat
     8101 ggcgtgtggt atcgctggtc tgtccgttgc tgctgactcc ctgtctgcaa tcaaatatgc
     8161 gaaagttaaa ccgattcgtg acgaagacgg tctggctatc gacttcgaaa tcgaaggcga
     8221 atacccgcag tttggtaaca atgatccgcg tgtagatgac ttggctgttg acctggtaga
     8281 acgtttcatg aagaaaattc agaaactgca cacctaccgt gacgctatcc cgactcagtc
     8341 tgttctgacc atcacttcta acgttgtgta tggtaagaaa actggtaaca ccccagacgg
     8401 tcgtcgtgct ggcgcgccgt tcggaccggg tgctaacccg atgcacggtc gtgaccagaa
     8461 aggtgctgta gcgtctctga cttccgttgc taaactgccg tttgcttacg ctaaagatgg
     8521 tatctcctac accttctcta tcgttccgaa cgcactgggt aaagacgacg aagttcgtaa
     8581 gaccaacctg gctggtctga tggatggtta cttccaccac gaagcatcca tcgaaggtgg
     8641 tcagcacctg aacgttaacg tgatgaaccg tgaaatgctg ctcgacgcga tggaaaaccc
     8701 ggaaaaatat ccgcagctga ccatccgtgt atctggctac gcagtacgtt tcaactcgct
     8761 gactaaagaa cagcagcagg acgttattac tcgtaccttc actcaatcta tgtaattaga
     8821 tttgactgaa atcgtacagt aaaaagcgta caataaaggc tccacgaaag tggggccttt
     8881 tttagcacga gagccttttt tgtcagctat ctatacttta aggtgactgc caaaacagac
     8941 tcgacgtagc cttcgagctg cgcaccaaca cggcctcaga tgggccacat ctggagaaac
     9001 accgcaatgt cagttattgg tcgcattcac tcctttgaat cctgtggaac cgtagacggc
     9061 ccaggtattc gctttatcac ctttttccag ggctgcctga tgcgctgcct gtattgtcat
     9121 aaccgcgaca cctgggacac gcatggcggt aaagaagtta ccgttgaaga tttgatgaag
     9181 gaagtggtga cctatcgcca ctttatgaac gcttccggcg gcggcgttac cgcatccggc
     9241 ggtgaagcaa tcctgcaagc tgagtttgtt cgtgactggt tccgcgcctg taaaaaagaa
     9301 ggcattcata cctgtctgga taccaacggt tttgttcgtc gttacgatcc ggtgattgat
     9361 gaactgctgg aagtaaccga cctggtaatg ctcgatctca aacagatgaa cgacgagatc
     9421 caccaaaatc tggttggagt ttccaaccac cgcacgctgg agttcgctaa atatctggcg
     9481 aacaaaaatg tgaaggtgtg gatccgctac gttgttgtcc caggctggtc tgacgatgac
     9541 gattcggcgc atcgcctcgg tgaatttacc cgtgatatgg gtaacgttga gaaaatcgaa
     9601 cttctcccct accacgaact gggcaaacac aaatgggtgg cgatgggtga agaatacaaa
     9661 ctcgatggtg ttaaaccacc gaagaaagag accatggaac gcgtgaaagg cattcttgag
     9721 cagtacggtc ataaggtaat gttctaattt ccccgtaaag cggcaacttt attgagttgc
     9781 cgctttttta ttccgcgctt cattctgcta cagcgttttt aacacatctt atagtgcatc
     9841 taccatatcc cgtacctgag ataataacga ctgataatag ctcgcgtgaa gttcttcccc
     9901 atcgaatacc actgtattac acaagaaagt gacatcggca tcttcaatac caagataact
     9961 acacatattt tttaaataat cgcttatatt tttttcccag cccatctgga caaatgattc
    10021 tttgtctcct ccaaccagcg ccacccaacg aactttattg aatggtaatt tgtgcccatc
    10081 tccatatgag gtgtactggc aatagcggac actaccattt gttctttttt taagcag
//
LOCUS       DADLSA010000135        10088 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.135, whole genome shotgun sequence.
ACCESSION   DADLSA010000135 DADLSA010000000
VERSION     DADLSA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.135"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            61..5301
                     /locus_tag="JN691_003276"
     CDS             61..5301
                     /locus_tag="JN691_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244638.1"
                     /translation="MASTNAHQIKNNDQNSLCGLGDKIRRLTAGVCLFTQIFFPVMAT
                     AQNVVHAKPQTTVSSAPPLLENNTVPYTLGALESAQSVADRFGISLEELRRLNQFRTF
                     ARGFDNVRQGEELDVPATTSQKSHEQQNAVPPANGENTLENQIASTSQRVGTLLSQDM
                     NSEQASGMARGWASSEASGAMTDWLNNFGTARISLGVDEDFSLKNSQFDFLHPWYDTP
                     DYLLFSQHTLHRTDDRTQINTSLGWRHFTSSWMSGINLFFDHDLSRYHSRAGLGAEYW
                     RDYLKLSSNAYIGLTGWRSAPELDYDYEARPANGWDLRAEGWLPVWPQLGGKLVYEQY
                     YGDEVALFDKNDRQSNPHAITAGLNYTPFPLLTLSAEQRQGKQGENDTRFAVDLTWQP
                     SSSMQKQLNPDEVAGRRSLAGSRYDLIDRNNNIVLEYRKKELIRLSLLDPVKGKSGEI
                     KPLVSSLQTKYALKGYNIEAAALKAAGGKVSTSGKDITVTLPGYRFTNTPETDNTWSI
                     DVTAEDVKGNLSRHEQSMVVIQAPTLSQKDSLLSVNPLTVAADKKSTTTLTVTAHDSD
                     GTPVPGLALQTRSEGVQDITLSDWTDNGDGSYIQMLTAGTTSGSVTLTPQINGESAVK
                     ESIVVNIVPVVSSRDHSSITIDNVSYYAGDDIKVRVELKDDSNQPVAYQKEELVKAVT
                     VENSKPGATIVWHEEQPGVYAANYPAHKQGTALRAQLSLHNWNAPLQSHIYNIEANQN
                     KARVATLSATNNDVYADKKTFNTLTINVTDESDNPLTNHQVTFKNEKGSAEFVEPPQQ
                     NTDGYGVATINMVSQVAEENTISATLPNGFSQRIIAKFVSDSSTPKFKQLVADPDTII
                     AGNSQGSTLTAIVTDFHNNPLKDMKVNFVAPGGSQLDNTTATTDQSGIVRVHLTSSKA
                     GSYSVDASLEVDKNIHQSVTITVVPNREQSVMTLNAGSGSAIANNTNIVTLTASVKDV
                     YGHPLPDEDVKFTLPASMTGNLTLSSETARTDANGDAVVTLRGTKAGEFTVTATLTRN
                     NTVAYQQVTFIGDTNSAQLQPLTASLNSIVAGNSTGSTLTATILDAYQNPLKDQLVTF
                     QSNDVTLSETEVTTNTLGQATVTMTSNIAGQHNVVVSRKAQASDNKTFSLSVLPDESS
                     AKVISITGAEKTITVGKNITLRILVQDAFNNVIAGQRVRLSAQPTTNITIGDTAYTDN
                     NGYAYVNLLSTQPGVYQVTATLDNNSSSKVDVNVANGKLELTSSKPETTVHNSEGITL
                     TATARNARGELMPGQIITFSVTPEGATLSNTGEVLTDQSGQAKVTLTSDKVNVYTVTA
                     IMGKDVPVQSQVTVAVKADAKTAHVVSVVASPDTITADGIDSSTITSRVEDDYGFPVE
                     GVDVSHGLDTKGSPVVNIPTTRTDQSGQVTATITNTLAETLTVNVQVPGTANQSATIT
                     LVAGTADESKSILKSDVDTLKADYQQSAKLTLTLQDKYGNPIVTSDHLEFVQSGPFVN
                     FLKLSDIDYSQRNYGEYTVTVTGGKEGTATLIPMLNGVHQANLSVSLNLIHSIKEMSG
                     HVTANNHTFSTAKFPSEGFAGAYYTLNNDNFEAGKTVDDYMFSSSQSWVSVDASGKVS
                     FANIGDQTSVTISAVPRQGGTTYQTLIKLKGWWVNNGNHTNIWLAANALCHAKNDGYN
                     LPSITHLTSGENKRTQGSLYGEWGNVGAFSSNSQFTPGAYWTSESDDYSRHYYVQMLT
                     GMTGSDADSSPQLTACRKSL"
     gene            complement(5425..6111)
                     /gene="kptA"
                     /locus_tag="JN691_003277"
     CDS             complement(5425..6111)
                     /gene="kptA"
                     /locus_tag="JN691_003277"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HAY7244639.1"
                     /translation="MWKRFCAGLSCRKFINPVTMTLVNKNRKSRDENAMAKYNEKELA
                     DTSKFLSFVLRHKPEAIGIVLDREGWADINKLILCAQKAGKRLTRALLDTVVATSDKK
                     RFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQFLYHGTASRFLDEIKKQGLIAGDRH
                     YVHLSADEATARKVGARHGSPVVLTVKAQEMAKRGIPFWQAENGVWLTSTVAVEFLEW
                     SFMPTGVQKQ"
     gene            6258..6852
                     /locus_tag="JN691_003278"
                     /pseudo
     CDS             6258..6852
                     /locus_tag="JN691_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6849..7310
                     /locus_tag="JN691_003279"
     CDS             6849..7310
                     /locus_tag="JN691_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244640.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            7323..8495
                     /gene="iadA"
                     /locus_tag="JN691_003280"
     CDS             7323..8495
                     /gene="iadA"
                     /locus_tag="JN691_003280"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY7244641.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            8560..9471
                     /gene="hypT"
                     /locus_tag="JN691_003281"
                     /gene_synonym="qseD"
     CDS             8560..9471
                     /gene="hypT"
                     /locus_tag="JN691_003281"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY7244642.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(9464..9856)
                     /gene="iraD"
                     /locus_tag="JN691_003282"
     CDS             complement(9464..9856)
                     /gene="iraD"
                     /locus_tag="JN691_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAY7244643.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(9853..9936)
                     /locus_tag="JN691_003283"
     CDS             complement(9853..9936)
                     /locus_tag="JN691_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide"
                     /protein_id="HAY7244644.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         2659 a         2561 c         2486 g         2382 t
ORIGIN      
        1 gcattatatc tccgtgctag aaaaaacaag tttaattctg ttttttcacg gaaggtcact
       61 atggcttcga caaatgcgca tcaaataaaa aataatgatc aaaactcttt atgtggtctg
      121 ggtgacaaaa tacgccgcct gaccgccgga gtttgtttat ttacacaaat ttttttcccc
      181 gtcatggcga cggcacaaaa tgtggtacat gctaaacctc agacgacagt ttcatctgcc
      241 ccccctctac tagaaaataa tacggtgcct tacacgcttg gtgcgctgga atcagcgcaa
      301 agcgttgctg atcgcttcgg tatttcactg gaggagcttc gtcgtcttaa tcagttccgt
      361 acttttgctc gcggctttga taacgtgcgc cagggtgaag aactggatgt tccggcaaca
      421 acctcgcaga aaagccatga gcaacaaaat gccgtaccgc ctgcgaatgg cgaaaacacg
      481 ctggagaatc aaatagccag cacctcgcag cgagttggca ctctgctttc acaagatatg
      541 aatagtgagc aggccagcgg catggcgcgt ggttgggcgt cttcagaagc ctcaggcgcg
      601 atgactgatt ggttaaataa ctttggtact gcgagaatct ctctgggtgt ggatgaagat
      661 tttagcctga aaaattcgca attcgacttc ctgcatccgt ggtatgacac acccgattat
      721 ctgctcttca gccagcatac ccttcaccga acagacgatc gtacccagat caacaccagt
      781 ttgggctggc gtcatttcac ctccagctgg atgtcaggca tcaacctgtt ttttgaccac
      841 gacctgagcc gctatcactc ccgcgcgggg cttggcgcag aatactggcg tgattatctg
      901 aagttgagca gcaacgctta tattggcctg accggctggc gtagcgcacc agaattggat
      961 tatgactacg aagcccgccc ggccaacggc tgggatttac gcgcggaagg ctggttacct
     1021 gtctggccac aactgggggg aaaactggtc tatgaacaat actatggcga tgaagtggcg
     1081 ctgtttgaca agaatgatcg tcaaagtaac ccccatgcta ttaccgcagg cctcaactat
     1141 acccccttcc cgcttctgac tctcagtgcg gaacagcgtc aggggaagca aggtgaaaat
     1201 gacacacgtt ttgccgttga tctgacctgg caacccagca gttcaatgca gaaacagctt
     1261 aatccggacg aagtggccgg acggcgcagt ctggccggta gtcgttatga cctgattgat
     1321 cgcaacaaca acatcgttct ggaataccgc aagaaagagc tgattcgcct gagtctgctg
     1381 gatccagtga aagggaagtc tggagaaata aaaccgctgg tttcctcgct acagaccaaa
     1441 tatgccctga aaggctataa catcgaagcc gctgcgctga aagctgccgg aggtaaagtc
     1501 agcacgtctg gaaaagatat cacggtcacg ctgccaggtt accgcttcac taacacccca
     1561 gaaaccgata atacatggtc gatagacgtt accgccgagg atgtaaaagg taacctgtca
     1621 cggcatgaac aaagcatggt agttattcag gctccgacat taagccagaa agattctctg
     1681 ttatccgtca atccgctaac cgtggctgca gataaaaaat cgacgaccac attgaccgtt
     1741 actgcgcacg attccgacgg aactccggtg ccggggctgg cgctgcaaac ccgcagtgaa
     1801 ggcgttcagg atatcaccct gtctgactgg acagataacg gtgatggtag ttacatacag
     1861 atgctgaccg ccggaacgac atcaggttca gtaacactga cgccgcaaat taacggtgag
     1921 agtgcggtaa aagaatccat cgtcgttaat atcgtccctg ttgtctcatc ccgcgaccat
     1981 tcatcaataa caattgataa cgtatcgtat tatgccggag acgacatcaa ggttagggtg
     2041 gaactgaaag acgacagcaa tcaaccggtt gcatatcaaa aagaggaatt ggtaaaagcc
     2101 gttactgtcg aaaacagcaa acctggcgcc acgattgtct ggcacgaaga gcagccgggg
     2161 gtttatgccg cgaattatcc ggcccataag caagggactg cactaagggc acaacttagc
     2221 cttcacaact ggaatgctcc actgcaatca catatttata acattgaggc aaaccagaat
     2281 aaggctcgcg tcgccacatt atcagcgacg aataatgacg tttacgctga taaaaagaca
     2341 tttaataccc tcactatcaa cgtcactgat gagagtgata atcccctgac aaatcatcag
     2401 gtcaccttta agaatgaaaa aggaagcgcg gaatttgtcg aaccgccgca gcaaaatacg
     2461 gatggatacg gtgttgccac aatcaacatg gtaagccagg ttgcggaaga aaacacgatt
     2521 agcgccacgc tgccaaatgg tttctcacaa cggataattg cgaaattcgt tagcgattcg
     2581 agtacgccaa aattcaaaca actggttgcc gatccagata ccattattgc tggcaacagc
     2641 cagggcagta ctctgaccgc catcgtcaca gactttcata acaacccgtt aaaagatatg
     2701 aaagtgaatt ttgtggcacc tggtggctcg caactggaca acacgaccgc cacaacagac
     2761 cagtccggta ttgtgcgggt gcacctgacc agttcaaaag ctggtagcta ttccgtcgat
     2821 gcctcgcttg aggtggataa aaatattcac cagtcggtca cgatcaccgt ggtcccaaac
     2881 agggaacaat cggtaatgac cttgaatgcc gggtcgggca gtgcgatcgc taacaataca
     2941 aatatcgtta ccctgactgc cagtgtgaaa gatgtttatg gacacccgtt gccggatgag
     3001 gatgtgaaat ttaccttgcc agcctccatg accgggaacc tcacgctaag tagtgaaacc
     3061 gcccgcaccg atgcaaacgg tgatgccgtg gtcacattgc gaggcacaaa agcgggtgag
     3121 tttacagtta cggcgacgct gaccagaaat aataccgttg cttatcagca agtcactttt
     3181 attggggata caaacagtgc gcagctccag ccgctgactg cctcattaaa ttccattgtt
     3241 gcgggtaaca gtacggggag taccctgacg gcaacgatcc tggacgctta ccaaaatccg
     3301 cttaaagacc agttggtcac tttccagagt aacgatgtca ctctaagcga aacagaagtc
     3361 accaccaata cgctgggtca ggcgacggta acaatgacca gcaatattgc cggacaacat
     3421 aacgtcgtgg tgagccggaa agcgcaagct tccgataata aaacgtttag tttatcagtg
     3481 ctgccggatg aaagttcggc gaaggtaata agtataaccg gagccgaaaa aacgataacg
     3541 gtgggcaaaa acatcacgct acggatactc gtccaggacg cgtttaacaa tgtaatcgcg
     3601 ggtcaacgcg tcagattaag tgcgcagcca acaactaaca ttacgatagg cgatacggct
     3661 tacaccgata ataacggtta tgcgtacgtt aaccttctca gcacccaacc tggggtttat
     3721 caggtgacgg caacgctgga caataacagt agtagtaagg ttgacgtgaa tgtggcaaat
     3781 ggcaaactcg agttaacatc atcgaaacca gaaaccacgg tccataatag tgagggtatt
     3841 acgctgaccg caacggcgag aaatgcgcgg ggtgaattga tgccagggca aattatcacc
     3901 tttagcgtaa cgcctgaagg tgcaacgcta agcaatacag gggaagtcct tactgaccag
     3961 tcaggtcagg ccaaagtgac gctgaccagt gacaaagtga atgtctatac cgttacggcc
     4021 ataatgggca aagatgttcc cgttcagagc caggtaacgg ttgcggttaa ggcagatgct
     4081 aaaacggcac atgttgtgag cgtcgtggct tctcctgaca ccatcaccgc cgacggcatc
     4141 gatagcagca ccatcacttc acgagtagaa gatgattacg gattcccggt tgaaggtgtc
     4201 gatgttagtc atggcttaga caccaaaggc agcccggtag ttaatattcc aactacgcgt
     4261 accgatcagt ccgggcaagt cacggcgaca ataaccaata cattggcaga aaccttaaca
     4321 gtcaatgtgc aagttcctgg tacagccaac caatccgcaa ccattacatt ggttgccggc
     4381 acggccgatg aaagtaagtc aattttgaaa tccgatgttg acactctgaa ggctgactac
     4441 cagcagagcg caaaacttac gctaacattg caagacaagt acggtaaccc gatagtgacg
     4501 tctgatcatc tggaatttgt ccagtcaggc cccttcgtga actttctcaa gttgagcgat
     4561 attgattaca gccaaagaaa ttatggcgag tacaccgtga ctgtcactgg cggaaaagag
     4621 ggaacagcga ccctcattcc catgctgaac ggggttcatc aggcaaactt aagcgtatcg
     4681 ctgaatctca tccactcgat aaaagaaatg tccggtcatg tcactgcaaa caaccatacc
     4741 ttctccacgg ctaaattccc gagcgaaggc tttgcagggg cgtattacac actcaacaat
     4801 gataactttg aagcgggtaa aaccgttgat gattatatgt tttcaagttc acagagttgg
     4861 gtgtctgtcg atgcttcagg taaggtttct ttcgcaaata tcggcgatca aacgtcagtc
     4921 acaataagcg ctgttccccg acaaggaggt acaacctacc agaccttaat taagctgaaa
     4981 ggctggtggg tgaataatgg aaatcatacc aatatctggc tagctgccaa tgcgctctgt
     5041 catgctaaaa atgatggata taatcttcct agcatcacac atttgacgtc tggcgaaaac
     5101 aaacgcacgc agggatcgct gtatggtgaa tgggggaacg ttggagcgtt ttccagtaat
     5161 tcgcaattta caccgggagc ttactggaca agtgaatctg atgattacag tcggcactac
     5221 tatgtgcaga tgctaaccgg tatgaccgga agcgacgctg attccagccc ccaactgacc
     5281 gcctgccgta aatcacttta atttcagcag ttgttattaa aggataccct tttacgaggg
     5341 tatcccttca gactgataac aaacgctaca ttgccggatg tgctgcgctt accaggccta
     5401 tataacccct gcaatatatt gaatttattg tttttgtacc ccagtcggca taaacgacca
     5461 ctcaagaaac tcaacagcaa ccgttgatgt cagccagacc ccgttttccg cttgccagaa
     5521 agggatgcct cgtttcgcca tttcctgtgc tttgacggtt aagacaaccg gcgatccgtg
     5581 tcttgctccc actttgcgtg ccgttgcttc atcagcagag agatgcacgt aatgacgatc
     5641 ccctgcaatc agcccctgtt tttttatctc gtcaaggaaa cggcttgccg taccgtggta
     5701 gagaaactgt ggcggtgttt tttcggcaaa ggaaatagcg acctgcgaag tcgaatgccc
     5761 ctgaaccgcg cggatgcacc taccatcact ggaataacta aaacgctttt tatcgctggt
     5821 tgcgaccacg gtatccagca atgcacgggt taatcgcttg cccgcttttt gggcacaaag
     5881 tatgagctta ttgatatccg cccatccttc acggtccagt acgataccaa tcgcctccgg
     5941 tttgtgtcgg aggacaaaac ttaaaaactt gctcgtatcc gccagctctt tttcgttgta
     6001 ctttgccatc gcgttttcgt ccctggattt tctgttttta tttactaatg tcattgtcac
     6061 tgggtttatg aattttctgc aactcaatcc tgcgcagaac cttttccaca cagcgtaatt
     6121 tttccctatg caaaaaacgc atagccagaa acatacttgc attttcccca tacccacatg
     6181 gctgataagg tttttcagtt ccaggaattt tcctggctca agcaacacac aaacacaaaa
     6241 aaacaatggg gattgttatg acacaacaag gggatgctgt agccggggag ctggcgacag
     6301 aaaaagtggg cattaaaggg tatctggctt tttttctcac cataatcttt ttttccggtg
     6361 ttttttccgg aacagacagc tggtggcgcg ggggctaaag atggctttct ctttgcgctg
     6421 gagctggcac cgtcagtcat tctctctctg gggattattt caattaccga tggtcttggt
     6481 ggattacgcg ccgcgcagca gttaatgaca cctgtgctaa aaccgttgtt gggcattcca
     6541 gggatctgct cgctggcact gatcgctaac ctgcaaaaca cggatgccgc cgccggtatg
     6601 accaaagagc tggcgcagga aggtgaaatt accgaacgcg ataaagtgat tttcgccgca
     6661 tatcaaacca gcggtagcgc catcatcacc aactacttct cttccggagt cgcggtgttt
     6721 gcctttctgg gcacgtccgt aatcgtacct ttagccgtca tcctggtgtt taaatttgtc
     6781 ggagccaaca ttttgcgcgt ctggcttaac ttcgaagaac gtcgcaaccc aacgcaagga
     6841 gcgcaagcat gacaactcag gtccgcaaaa acgtcatgga tatgtttatc gatggcgcgc
     6901 gccgtggctt tactatcgcc acgaccaacc tgctgccaaa cgtggtgatg gcgtttgtca
     6961 tcattcaggc gctgaaaatt accggcctgc tcgactgggt ggggcatatc tgtgaacccg
     7021 taatggcgct gtggggatta cccggtgaag cggcaaccgt ccttttagcc gcattaatga
     7081 gtatgggcgg cgcagtcggt gttgccgcca gtctggcaac cgctgacgca ttaaccggcc
     7141 acgatgtcac cgttttactg cccgcaatgt atttaatggg caacccggtg caaaatgtcg
     7201 gtcgctgcct ggggacagcc gaagtgaatg ccaaatatta cccgcatatc attacggtct
     7261 gcgtgattaa cgcattgctg tcgatctggg ttatgcagct tattgtttaa caaggagtta
     7321 ccatgattga ttataccgca gccggtttta ccctgctgca gggagcgcat ttgtatgcgc
     7381 cggaagatcg gggaatttgc gatgtcctcg tcgctaacgg caaaattatc gccgttgcca
     7441 gcaatatccc ttctgacatt gtaccgaact gcacggttgt cgatctcagc gggcagatcc
     7501 tctgcccagg ttttattgat caacacgtcc atttgattgg cggtggcggc gaagcaggtc
     7561 ccacgacgcg cacgccggaa gtggcgctaa gtcgcctgac ggaagcgggc gtcacgtcag
     7621 tggttggtct gctgggcacc gactctatct ctcgccaccc ggaatccctg ctcgccaaga
     7681 cccgtgcgct caatgaagaa ggcatcagcg cctggatgct gaccggcgct tatcatgtcc
     7741 cttcccgcac tattacgggt tccgtggaaa aagacgtggc gattatcgat cgtgtgattg
     7801 gcgtgaaatg cgccatctct gatcaccgtt ctgccgcacc ggacgtttat cacctggcta
     7861 atatggcggc agaatcccgc gttggcggtt tgctcggcgg taaacctggc gtcaccgtgt
     7921 tccacatggg cgacagtaaa aaggcgttaa agcctattta tgacctgctg gaaaactgcg
     7981 atgtgccgat cagcaagctg ctgccgaccc acgttaaccg caacgtaccg ttgtttgagc
     8041 aggcactgga gttcgcgcgc aaaggcggca ccatcgatat caccagcagc attgacgaac
     8101 cggtcgcccc tgccgaaggt attgcccgcg ccgttcaggc gggtattccg ctggcacgcg
     8161 tcaccctcag ctccgacggc aacggtagcc agccgttctt cgatgacgaa gggaatttaa
     8221 cccatatcgg tgttgcaggt tttgaaacgt tgctggaaac cgtgcaggtg ctggtcaaag
     8281 actatgattt cagtatcagc gatgccctgc gcccgctcac cagtagcgta gccggtttcc
     8341 ttaacatgag cgggaaaggc gaaattctgc caggcaatga cgcagactta ctggtcatga
     8401 cgccagaatt gcgcattgag caggtatacg ctcgcggcaa actgatggtc aaagacggca
     8461 aagcctgcgt gaaaggaacg tttgaaacgg cttaaacggt tgtcagccgc cctaatggac
     8521 gtagaatgcc cccaagggcg gctgacagag taaaacgtaa tggatgactg tggtgcggtt
     8581 ttgcataata ttgaaaccaa atggctttat gattttctga ctctggaaaa atgccgcaat
     8641 ttttcccagg cggcagtcag tcgcaacgtc tcgcaaccgg cattcagccg ccgcatccgt
     8701 gcactggaac aggcgattgg cgttgaattg tttaaccgcc aggtgacgcc actgcaactc
     8761 tcagaacaag ggaaaatctt ccattcgcag atccgccatc tgttgcaaca gttagaaagc
     8821 aaccttgcag agctgcgtgg cggcagcgat tacgcgcaac gtaaaatcaa gatagccgct
     8881 gcacactctc tttccctcgg tctgttaccg tccattatca gccagatgcc gccgctcttt
     8941 acctgggcaa ttgaagccat tgatgtcgat gaagcggtcg ataaactgcg tgaagggcaa
     9001 agtgactgta ttttttcctt tcacgacgaa gatttgctgg aagcgccgtt tgaccacatt
     9061 cgcttatttg aatctcaatt gttccctgtc tgcgccagtg acgaacacgg agaagcactt
     9121 tttaacctcg cgcagccaca ctttccgtta ctgaattaca gccgcaactc ctacatgggg
     9181 cgattgatta atcgcaccct gacgcgccac agtgagttaa gtttcagcac cttttttgtc
     9241 tcttcgatga gcgagctttt aaagcaggtt gccctcgacg gctgtgggat tgcctggctg
     9301 ccggagtatg ccatccaaca agaaattcgc agcgggcagc tcgttgtgct gaatcgggac
     9361 gaacaggtga tcccgattca ggcctacgca taccggatga atacccgcat gaatccagtt
     9421 gccgaacgct tctggcgtga actgcgcgag ctggagattg tgcttagctg acattctcaa
     9481 gcgtcgcact gcgccaaccg ccattatccc agaccagatc aaacgttagc ggttcatcgt
     9541 cgtggtacag cattatctca aggctgatcg ccagagtgtg gcagtacgct tctttcaaat
     9601 taacctgcaa cgccgcaatc cgtggctcat gaaataacag tgcagaacgc agctgacggc
     9661 aataccagct ctggagatgt ttgtcgctcg ggttatgcac ttgccactcg ggcatgcctg
     9721 aaacaagtaa tgacggtaat gccgaatgag gagaattaaa aagagtgagg agatcgctgc
     9781 ataccgattt acgcagcaat gacgttttat tgcctgaaat ttcaggtaaa acagcctgaa
     9841 gtgattggcg catcattttg cgcactcctg acgtttagca acatatgcgg cttgccctga
     9901 acaccgggca ccactgtatt gatgctcatt ttccatagcc attccctttt gttcattaca
     9961 tccatgtcag tctgagacag gcctgaatcc tgtcagcaaa gtataaaacc ttacttgcct
    10021 atgaatatct actgactttt caggaaaaaa gtcatgattt gataaaaatc atcaaactca
    10081 aaaaaaaa
//
LOCUS       DADLSA010000136        10073 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.136, whole genome shotgun sequence.
ACCESSION   DADLSA010000136 DADLSA010000000
VERSION     DADLSA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10073
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.136"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..379
                     /gene="fliR"
                     /locus_tag="JN691_003284"
     CDS             <1..379
                     /gene="fliR"
                     /locus_tag="JN691_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="FliR, with proteins FliP and FliQ, forms the core
                     of the central channel in the flagella export apparatus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HAY7244645.1"
                     /translation="FLTFNGHLWLISLLVDTFHTLPIGSEPLNSNAFLAPTKAGSLIF
                     LNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAP
                     FCKHLFSEIFNLLADIISELPLI"
     gene            669..1292
                     /gene="rcsA"
                     /locus_tag="JN691_003285"
     CDS             669..1292
                     /gene="rcsA"
                     /locus_tag="JN691_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAY7244646.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(1336..1578)
                     /gene="dsrB"
                     /locus_tag="JN691_003286"
     CDS             complement(1336..1578)
                     /gene="dsrB"
                     /locus_tag="JN691_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAY7244647.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVSLEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            1687..1914
                     /gene="yodD"
                     /locus_tag="JN691_003287"
     CDS             1687..1914
                     /gene="yodD"
                     /locus_tag="JN691_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HAY7244648.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            2212..3027
                     /locus_tag="JN691_003288"
                     /pseudo
     CDS             2212..3027
                     /locus_tag="JN691_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(3024..4718)
                     /gene="yedQ"
                     /locus_tag="JN691_003289"
     CDS             complement(3024..4718)
                     /gene="yedQ"
                     /locus_tag="JN691_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HAY7244649.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNFLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGICMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(4889..5071)
                     /locus_tag="JN691_003290"
     CDS             complement(4889..5071)
                     /locus_tag="JN691_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAY7244650.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(5150..6067)
                     /locus_tag="JN691_003291"
     CDS             complement(5150..6067)
                     /locus_tag="JN691_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAY7244651.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6240..7160
                     /gene="yedA"
                     /locus_tag="JN691_003292"
     CDS             6240..7160
                     /gene="yedA"
                     /locus_tag="JN691_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAY7244652.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(7149..7619)
                     /locus_tag="JN691_003293"
     CDS             complement(7149..7619)
                     /locus_tag="JN691_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAY7244653.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(7600..9018)
                     /locus_tag="JN691_003294"
     CDS             complement(7600..9018)
                     /locus_tag="JN691_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAY7244654.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(9085..9780)
                     /locus_tag="JN691_003295"
     CDS             complement(9085..9780)
                     /locus_tag="JN691_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAY7244655.1"
                     /translation="MELQHWQAQFENWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGLALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLGAELAGENEG
                     IDHKVIDAFSPAG"
     gene            complement(9820..10017)
                     /locus_tag="JN691_003296"
     CDS             complement(9820..10017)
                     /locus_tag="JN691_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244656.1"
                     /translation="MARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVG
                     ALLGGIAWYTRPRPARCRRYE"
BASE COUNT         2473 a         2652 c         2409 g         2539 t
ORIGIN      
        1 gttcctgaca tttaacggtc atttatggtt gatttcactg ctggtcgata cctttcacac
       61 cctgccgatt ggtagcgaac cgttgaacag caatgcgttt ctggcaccca ccaaagcagg
      121 gagtttgatt ttccttaacg ggctgatgct ggcgttaccg ctcattactc tgttgctgac
      181 cctgaatctg gcattaggtt tactgaatcg tatggccccg caattatcca tttttgttat
      241 tggatttcca ttaactctga ctgtcggcat ctctttaatg gcggcattaa tgccgttaat
      301 tgcgcctttt tgcaaacatt tattcagtga aatttttaat ttgctggctg atattattag
      361 tgaattacca ttaatataat tccgtaacgt ttatcatgct accctaagga ttatccgaaa
      421 aataatatct gcgaacatct tccaggatac tcctgtagcg aaatatttgt tttaagctca
      481 ctcacatatc gcaacattta ctttacttta agacaattcc aggcaaatta tacaacactt
      541 tacgggatag taagtccgcc tgaaaaattg cgagagtggc gcattaggtg acccatgttg
      601 ttccgtttag tcatgatgaa atattcaggt aaggggaatt atcgttacgc attgagtgag
      661 ggtatgccat gtcaacgatt attatggatt tatgtagtta cacccgacta ggtttaaccg
      721 ggtatctgtt gagtagaggg gttaaaaaaa gagaaatcaa cgacattgaa accgttgatg
      781 accttgccat agcttgtgat tcacagcgcc cttcagtggt gtttattaat gaggactgtt
      841 tcatccacga tgcttctaac agtcagcgca tcaagcacat cattaatcaa catcccaata
      901 cgttatttat cgtttttatg gcaattgcca atgttcattt tgatgaatat ctattggtca
      961 gaaaaaattt attgatcagc tctaaatcga ttaaaccgga atctctcgac gatatccttg
     1021 gcgatattct gaaaaaagag acaacgataa cctcgttttt aaatatgccg acgttatcat
     1081 tgagccgaac cgaatcgagt atgttgcgaa tgtggatggc aggtcaggga accattcaaa
     1141 tctccgacca aatgaatatc aaagccaaga ccgtttcatc gcataaaggt aatattaaac
     1201 gtaagatcaa aacgcataat aaacaggtta tctaccatgt cgtccgactg acggataatg
     1261 tgactaatgg tatttttgtc aacatgcgct aacacattct gactggtggt ttccctccag
     1321 tcaggctgaa taagattact ctgctttctc aacaaagata ccgtcctgat gccctgcttc
     1381 attaaagaac cagatgccga gcgggtagtc ttccagcgaa accaggtaca ttgtgccttc
     1441 actaaactcc tcaactgcca gtaccactcc aggacgacgc ggaccgccat ccgttttgac
     1501 tgttacccga tcattcacct tcatcgtttt cctcctgtgg ctttgtgcca gtgtagaaca
     1561 atttcgtttt tctggcagcg ccaggcgcgc gcgagtgctg attttctcga cggtctatac
     1621 ttaagagatg ccaggcggac ttaacgactg gcggcaacaa cagagtaacg gttgcgagga
     1681 aagatgatga aaaccgcaaa agtgtacagc gataccgcaa aacgtgaggt cagcgtcgat
     1741 gtcgatgccc tgctggcggc gatcaatgaa attagcgaaa gcgaagttca tcgcagccag
     1801 aacgattctg aacacgttag cgtcgatgga cgtgaatatc atacatggcg tgaattggcg
     1861 gatgccttcg aactggatat tcatgacttc agcgtctctg aagtgaatcg ttgaatgcac
     1921 aataaaaaaa tcccgaccct gagggtgtcg ggatgaaact tgcttaagca agaagcactt
     1981 aaaaaattcg ttacaccagg aaatctgatg tgttcatcac cttatctgca atttttttcg
     2041 ctgacaagaa aatattcgcg ctatgaatga ttaacgctac ttatgaatag aaatgtgacg
     2101 cgcgtcaccc cttggtggct ctggtgtcaa cgggatcgct cccgaaaaaa tcatctttga
     2161 ttatttgtgc tgttttgaga atcatcctgg taaggggagt actgcgcgac catgttttca
     2221 attcaacaac cactactggt ttttagcgat cttgatggca ccctgctgga cagtcatagt
     2281 tatgactggc aaccggcagc cccctggctc agccgtttac acgaagccaa tattcccgtc
     2341 attctctgta gcagtaaaac ttcagcggaa atgctgtact tgcaaaaaat gttggggcta
     2401 caaggcttac cgctgattgc agagaatggc gcagtgatcc agcttgctga acaatggcag
     2461 gatatagacg gttttccacg catcatctca ggtattagcc atggcgaaat ctgccaggtt
     2521 ttaaatacgc tacgcgagaa agaacatttt aaattcacga cttttgatga tgtcgacgat
     2581 gcaaccattg ccgaatggac gggattaagc cgtagctagg cggcgctgac gcagctgcat
     2641 gaggcgtcgg taacgctaat ctggcgcgac agtgacgagc gtatggcaca atttatcgct
     2701 cgtctgaacg aactgggctt gcagtttatg cagggtgcgc gcttctggca cgtactggat
     2761 gcctctgccg gaaaagatca ggctgccaac tggattatcg cgacctatca acaattgtca
     2821 ggcagacgcc caaccacact tggcctgggc gatgggccaa acgatgcgcc tttactggag
     2881 gtaatggatt acgcggtgat tgtgaaaggg ctaaaccgtg aaggggtaca tctgcatgat
     2941 gaggatccgg cccgcgtctg gcgaacgcag cgtgaaggac cggaaggctg gcgtgaaggg
     3001 ctggaccatt ttttctccgc ccgttaagcg ttatcgctcg cgaatacccg attgcgcccg
     3061 gcctgttttg cgaggtaaag ccgacggtcg gccaatgact ggagttgttc aaaatcataa
     3121 tcaccggttt cctcgctgct acttaccccc agcgaggcac tgatgcgtat cgttgtactc
     3181 ttggcgatca gcatctcttt ttcatttaac ttcaggcgaa tacgttctgc gacctccgca
     3241 gcctgcgtca gattcgcgcc tggcagaatc acacaaaact cctcaccacc gacccgcccg
     3301 gcaacgtcct gcgcacgcaa ggaactgcta attaatccgg cagcatgaga aagaacacgg
     3361 tcgcccgcct gatgaccaaa gcggtcatta atcgctttaa aatggtcaag atcgacctga
     3421 atgacagaaa aaggatgttg gtgcgtctga cacaatttag cgagcggacg ggctttttcg
     3481 aacagtgcgc cacggttata taaacgcgtt aaggtgtcgt gccacgcctg ccactgcaac
     3541 gagctttgca gaacatacat gttgctgacc atccggcgaa tcacatacca ggagaggagt
     3601 aacatggtgg taaagagcgc ccacagcagg gttaatgcaa tgctgatact gccgaaatcg
     3661 ccgcgcacgc cttcgcttag cgtatggacg cgcaccagca caccgtcgaa atggtccaga
     3721 cgttcccagc taacatagcg actgtccata caaatgccgc cccgcgtgtc atgttccatt
     3781 gcctgcgcca gcaaggccag ttcacgagga tcaaaaatat tccctgttgg atggtcagga
     3841 ttggaagagg tcaaaaatct cagcttactg tcatagagct gatactcacc atcgaggttt
     3901 ttatcgatgg cgtttcttaa aaattgctgc atagtacgca cgggaatact catccccagc
     3961 acgccatacc agtagttatt actgtctacc ggaacactga cggtaacctg cggttcagta
     4021 ttgctggcgt gttccggttg cgaagtgaac cagcgtaccg cgcggtgacg attttctcgt
     4081 tgcgaatggc cgataaacca gggttgggtg acatagccgt aataacgcgt tggtacattg
     4141 cgcgtaaaca aggtcggctg cgtcgaaacg taaaatccag cacgcgagac atacatcgcc
     4201 tgttcaacca tcgacgagga gttgtgcgcc agtcgcagca agtaaccaac ttccagtgca
     4261 gcggtaattt cattgtcgag gctttcattt tcgcgagaca ggaaattccc ctcgctgact
     4321 aatgcatccg atacgccatt gactggcaag gtgcgccgtc ggttgagttc gatttgccag
     4381 gcgtgctcgt cgcgatgctg ttcgaactcg gttacggcat tacgcagaga ggtgaaatcc
     4441 agtggcgcca cgagagcttc gcgcatgcca ttacgcaaaa agatcagttt gtcgacgtta
     4501 tactgcaaat gcttatcgag cgcgttggct acgttttcca gatgattacg ctggctggag
     4561 atataggcat cttccagcac caccacttca cgccaggtga gcaaggtgga aaaaagcaat
     4621 accacgataa agcagagatt aacgacatga ccgggcccca ggcggcgtgc gagttttttc
     4681 aaccagctct ggttttccat ttttgtctcg tgctgcaccc tgacgactcc caacctgctt
     4741 ttttatgatt ctggcataac ccggcgatag cgtaccgcaa aacagcgata attttataat
     4801 ggtggcatga taagacgcga ctatcattca ttgcaggcat tgggacacca ggccggatgt
     4861 gatccggcct ggtgttaatg ataccctttc aaacttcttc cgcagaacga ctcccctccc
     4921 ccggaacaag atcggtttca tgaaaagctt cccgcttgac cccgtaaccg tcataccaac
     4981 gacactcaac cataccgctg gagtatccag tgacaatcat ccgcgggccg ccctctttaa
     5041 ccgtaacttc ctcactaacc ataaagctca tactcgcctc cttttttcga gtgaaacttg
     5101 ttcaccttag ttgaagatgg cgaattttgc ataatagcca gtgcgagtat taatgtgcct
     5161 gaccgcgcat tttcgctact gctttcactc ccagcactac gatgccgccg atgataaatc
     5221 caagaatcag atttaaaaca gtcggtaata tcatcgccac cactgcactt tgctgcccgg
     5281 cgatatgttc aatagcgtga tgcagcggcg caataccatg taccacaatc ccgccgccga
     5341 cgaggaacat cgccagcgtg ccgacaatcg ataatgcttt catcagccaa ggcgcgataa
     5401 tcaataaccc tttacctaat gcctgcatca gcgcgctgga tttttccgcc agccaatacc
     5461 ccaggtcatc aatcttaacg ataaccccta ccagaccgta gacgcccacg gtcactacca
     5521 gcgcgatgcc tgaaagcacc agcacctgat taagcaacgg cgcctctgcc acaatcccca
     5581 gcgtgatggc gacgatttcc gcagacaaaa taaaatcggt acgaatcgcc cctttaattt
     5641 tgtccttttc aaacttcagc ggatcctgcg ccgccagctt ctccagacgc tgctggctct
     5701 gcgctggatc ttctttatgt ttacgcgcct ccagcatatg cagcactttc tctacccctt
     5761 caaagcagag aaacgcgcca ccaatcatca acagtggcgt aatcgcccac gggataaacg
     5821 cactgatgat cagcgccagc ggcaccagaa tcactttatt gatcagcgat cctttcgcca
     5881 cgccccagac cacgggaagt tcccggttgg cccgcacgcc tgaaacttgt tgcgcattga
     5941 gcgataaatc atcccctaat acaccggcgg ttttcttcgc cgccagtttg cccatcacag
     6001 agatatcgtc cagcagtgtg gcgatatcat cgagcaacgt cagtaaacta ctcccggcca
     6061 aaagcattcc ctctcatttt tattgttaaa gggtgtaagt atgaagcaaa aacggcagct
     6121 ctgatacttg cttcacaggt caacatttat ttaacaatca agtgcaatta tatctctcgc
     6181 cagcagaatg attttcacgt ttactatgaa ccaccttttt atttccgccg tgagggatta
     6241 tgcgtttccg ccagttgtta ccgctttttg gcgcgctgtt tgcgttgtat atcatttggg
     6301 gctcaaccta ttttgtcatt cggattggcg tgggaagctg gcctccgtta atgatggcgg
     6361 gcgttcgatt cctggcagcc ggtattttat tgctggcatt tttgctactg cgcggacaca
     6421 aacttccccc gctacgtccg ctgctcaatg ccgcgctgat tggcctgtta ttgctggctg
     6481 tcggtaatgg catggtgacg gttgccgaac atcaaaatgt tccttccggc atcgccgccg
     6541 tagtgattgc aaccgtgccc ctctttaccc tgtgcttcag tcgcctgttt ggcattaaaa
     6601 cgcgcaaact ggaatgggtg ggtattgcca ttgggcttgc cggaatcatc atgctcaata
     6661 gcggtggaaa tttaagcggc aatccgtggg gcgcgattct gattttaatc ggctcgatta
     6721 gctgggcgtt tggctcagtt tatggctcgc gtattacctt acctgtaggg atgatggcgg
     6781 gtgcgattga gatgctggcg gcaggcgtgg tgttaatgat cgcatcgatg attgcgggtg
     6841 aaaaactgac ggcgctccct tccctgtcag gcttccttgc ggtcggctat ctggcgctgt
     6901 ttggttcgat tatcgccatt aacgcttata tgtatttaat ccgtaatgtc agtccggctc
     6961 tcgccaccag ctacgcctac gttaacccgg tggtcgcggt cttgctgggt acgggactgg
     7021 gtggcgaaac gctgtcgaag attgaatggc tggcgctcgg cgtaattgtt ttcgcggtgg
     7081 tactggtcac gttgggaaaa tatctcttcc cggccaaacc tgttgtcgcg ccagttattc
     7141 aggatgcatc aagcgagtaa atgaatcccc tgcgtgtcga tctgcgcgct ggcaccttcg
     7201 ccgcagatcc actcttccag acgctcggta agcgccgcat ccgtcagctt ctcgcgccta
     7261 cgtaacgcac actcccagac aatcagtacg cgccagccga gctcctgcaa gcgactgata
     7321 tcgcggcgat cgcgttcaac atttttacct atcttctcca gccagaactc agttcgagtc
     7381 gcaggcactt taaacagata gcagtgatga tgatgccaga agcagccgtg ggtaaatatc
     7441 acgcagcgat attcatcaac gacaaaatcc gggcgtccgg gcagactggc gtcctgaacg
     7501 cgaaacgcaa ggccctgccc ggttaacaaa ctggcgaggc gcttctcaat cgccgtatca
     7561 cgcgtggcaa tcgcgcgcat atttttgctg cgggtggcct tatcgtgaac gtcgaccatg
     7621 ttgtgcctct tgctgacgca acgccaccgc ctgtttgatt tttggctcaa gcagttttgc
     7681 caccgcggca aagaccggca cgaccaccga gttgccgaac tggcgatagg cctgagtgtc
     7741 cgaaaccgga atgcggaatt tcgcttctcc cggcgcttca aaacccatta agcgcgcgca
     7801 ttcacgaggc gttaaccgac gtggacgatg ttgctgattc agcggatcgt caaagtcttt
     7861 ctcacccgtg gccatatccc aaccgcgatc gattaaaatt tccgcgccat ctttgtaata
     7921 acgagcagac agcgtacggg tgacgctttg cggattgttc ggataaacca ttccataacc
     7981 gaagccgtta ccgcgcgcct gatgtttttt cgcatatcga tagagatact tccacagcac
     8041 cggcgtcagg atatatttcg cctcaaccat cgggtccagc agctgcgcca gcgtcactcg
     8101 ctgtgcaggg aaacattcgc tgatatcacg cagagtaaaa tcggctttca gattcagatc
     8161 gcgacgaaaa cccaccagca cgatgcgttc acggtgctgc ggcaggaaat gtttaccatc
     8221 gatgattttc ggatcgtccg gcccgttatc ttctgcatcg gcaacgtcat agcccagttc
     8281 gtccagcgtc tgcatgatga tgcggaacgt ttttccctta tcgtgactct tcaggttttt
     8341 gacgttttcg agcacaaaca tcgccggacg acgcgcgtcg ataatgcgca ccacatcaaa
     8401 gaacagcgtg ccctgggtat cgcaggcaaa accgtgcgcc cgcccgagcg agttcttttt
     8461 cgatacgcca gccagcgaaa atggctgaca agggaaaccg gccagtaaaa catcgtgttc
     8521 aggaatgtgt tgacgaatat gttccgccgc cgcctcatca ctcacgcctt cttgatgact
     8581 gagggtgatg tcgcggatat cttcattaaa atgatgcgtc gccgggtcgc aataatggtt
     8641 ggctttataa gtgcgtaccg catgtttgtt ccattcgctg gtaaacacgc actgtccacc
     8701 aatcgattca aaaccacgac ggatgccgcc aattccggca aatagatcga taaagcgaaa
     8761 cgcataatgc ggatgatgtt ccggtggttt gggtaacaac gtttgcagat gggcaaactc
     8821 tttctcactt aaacggtgcc atgccgagtc attcgccagc gcacgtttta aaattgccgc
     8881 gctccagtga ttctcaccca caccattaag ctgcgccacc agcattttaa catcataaat
     8941 ttgcagcagt ttctccagca ttgcctgtgc ggcattcccg gtgctgtatg aatcggttac
     9001 tgatatattt tcctgcatag atttaaccgg ccatctgaga tggccgacag gttaacataa
     9061 ttactgccgt ttagggccac gcgctcagcc agcgggtgaa aacgcatcta tcaccttgtg
     9121 atcgatacct tcattctccc ccgccagttc ggcaccgagc ttcgccataa actcgactaa
     9181 aaattgcgcg ttgtgctgtg ccagctgctt gcctcttgcg gtttgcatgg tttgcggtaa
     9241 tttcagtaac tttgtctgga aatgatccag cgcgtagcgt ttatcatcaa gcgggcgatg
     9301 ctgtgcgaac ggatcttcgc catcaaacag cgccaagccc aatgcccctg aaacggcaaa
     9361 tacgcgcgcc aggccaatcg cccccagggc ttccagccga tccgcatcct ggacgatttt
     9421 agcttccgtt gttaaggggg cgatttgcgc gctgaaactg tgagcggcaa tggcatgaca
     9481 aacggcctca attttctccg ccggaaattg cacgaactct tcacggagca ggcgacgcgt
     9541 ctcttctgcc gccaggattg aagaacgctg ccgttgcgga tgatttttcg ccaggctcac
     9601 gatatcgtga aaataacagg cggttaaaat caccagcata tcgacatcgt catctgccgc
     9661 cagtttctgt gccgtagccc agacacggcg aaaatgacac aggtcatgcg ccgcgtcctg
     9721 atgttgatga tggtttttca accagttctc gaactgtgcc tgccagtgtt gtaactccat
     9781 acgcgctccg ttgccaaaga gtcgcaacct tagcagtttt tattcatagc gtctgcaacg
     9841 tgcgggacga ggtcgggtgt accatgcaat gccgcctaac aaggcaccaa ccagggccat
     9901 aaacagaaat ccgaccaatg ctcctaacca cttccccgcg gtagatccta aatcactttc
     9961 aaatcccgac acgggggcat cgggtatccg gctcatcacc cagatgtatc gcgccattgc
    10021 ccagacgaaa tagccacacc atgtatagaa agcccacaat gaatcgccac gga
//
LOCUS       DADLSA010000137        10013 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.137, whole genome shotgun sequence.
ACCESSION   DADLSA010000137 DADLSA010000000
VERSION     DADLSA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10013
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.137"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..186)
                     /locus_tag="JN691_003297"
     CDS             complement(<1..186)
                     /locus_tag="JN691_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HAY7244657.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINART"
     gene            complement(191..376)
                     /locus_tag="JN691_003298"
     CDS             complement(191..376)
                     /locus_tag="JN691_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAY7244658.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(373..3019)
                     /locus_tag="JN691_003299"
                     /pseudo
     CDS             complement(373..3019)
                     /locus_tag="JN691_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
     gene            3227..4216
                     /gene="ldhA"
                     /locus_tag="JN691_003300"
     CDS             3227..4216
                     /gene="ldhA"
                     /locus_tag="JN691_003300"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY7244659.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4327..4749
                     /gene="hslJ"
                     /locus_tag="JN691_003301"
     CDS             4327..4749
                     /gene="hslJ"
                     /locus_tag="JN691_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HAY7244660.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4746..5012)
                     /locus_tag="JN691_003302"
     CDS             complement(4746..5012)
                     /locus_tag="JN691_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAY7244661.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5286..8801
                     /gene="nifJ"
                     /locus_tag="JN691_003303"
                     /pseudo
     CDS             5286..8801
                     /gene="nifJ"
                     /locus_tag="JN691_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
     gene            9168..>10013
                     /gene="ompN"
                     /locus_tag="JN691_003304"
     CDS             9168..>10013
                     /gene="ompN"
                     /locus_tag="JN691_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HAY7244662.1"
                     /translation="MKRKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNERDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYVVAN
                     KTQNFE"
BASE COUNT         2546 a         2563 c         2455 g         2449 t
ORIGIN      
        1 cgttcgcgca ttaatttcgg ttactaattt ctctgtttca gcatccgttt tcagcgcaac
       61 cagataacca taaaatgttt cacctacccg cccctgagtt ctggcttcat ccagagtcaa
      121 tgccattaca ttgctgctta ccagcccaac aagaaacgca caaagaagta atgttttctt
      181 catcatcgcc tcagaaaaga tcgctacgag tttcaagcag ctcttcgaca tctttgtctg
      241 ccttgatgat gatctcatgc tcaattttaa cgttcatatt gatagtgatc ggttccttag
      301 gtgcagcgac ttcaatgcga ggcgtacagc caaccagcat aaaagatgac gtcaacgcag
      361 ccagtaaaat tttcattgtt tttcctcaca ctcttttcct tgcggacagt catttcccgg
      421 cagacgtgcg ttctgctcca accatgcctg gagattatcg ccaaagcgta aactgcgcca
      481 cagcgtaaaa atattctctt catgatggta attaaggttt accgtaccgc ttttaccatc
      541 aacgcgactg gtacctgtaa tgttggcctg catggttaat aaaccgagat tatctaagtt
      601 aatttttgtc gatgaccggc taatttccat atagcgcaac cagttaattg ctgaacccgc
      661 agtcatattg tctttcacca ccgcatctgc ggtatcttta tccagccgca aggtcattgg
      721 cccgctattt gccagccaac catctttcac tatccatttt tcgttgttta gccataacgg
      781 caatgcacca ctaaatgccc cggacatggc gaattgtttc ggattgacgg cgctaaccag
      841 ttcgctggat gagagattat tcagccgcaa cagcgccggg tcatgttgcg gcatacgtaa
      901 ttgtttcatc agcacgttac cgcctaacac atcgacgcta acatcagtca acagcaaggg
      961 ttcttcttca gtccacggat aacgcccttg caaatcagca gtaatatttt tcgctgtcac
     1021 cagattaatc acttcggcaa ttcgcaacgt aacggggccg cgagtcccca gatgccaggc
     1081 tccatcggca aaacggaaag gcaggacaaa atcgacaccg ttaacctgat tatctggcat
     1141 ccaggcactg ccgcctttca acacgccgtg ccctcccgcg cggaatcctt gttcaggcgc
     1201 agcagaaaat gcaacctgag catacagttc accatcgcgt aagttcatct tccagtcggg
     1261 tggcaccagc ggctggaata cagtcagtga ttgttttggc caccaggcgt taccgcgcag
     1321 acgaataccg tcccagcgac catttacccg aaccgggcca atctcaccag catgtaaatc
     1381 gcctttaaag aggaaatagg tcggatcgcg cccatcgacg ctaaatttta aggttgatgg
     1441 cggtaacaca ctgccgccag tgaacagcgt ttgcccggcg tccagtgaca gcgcgccgct
     1501 gaaggaggga tgctgtgcgt cacgtaccca gacgacgggc ttgtcgagaa ttaatcgcgg
     1561 cttttctacc gtcatcgtac cgtattgtaa ctggtcgaaa ccggtggaga gatcggtcag
     1621 cgtaatcgtg ctgtcatgcc actcaccttt tcctgcgaca tcccaggtgg cattcatcgg
     1681 tgtaaaactc ccttttcccc agtagcgcca ctgccagcgg ccagcgtcca gcgtcaggga
     1741 gaaaatcatt cgccagccca tccatatgca gcacgaaatc gcccagttca ttttcatgcg
     1801 cctgcaaaat ggcctgcaaa cgtccgtcaa caccacgttg ggtgactttt acacccgcta
     1861 aaggccagcg gatttcatcg atgtccagcg aatcgatgac tcttcccttt gaacgaagta
     1921 acgcgcctgg ctcaaaggtc agcgttgggt cagacagact tccacttagc tgcgcaggta
     1981 aacgggcata taaaatgagg tccgcctgtt tcgcttcacc ggtcagctgc agaggcagtt
     2041 gactgttatc catgcttaat tttcctgggc caaaattaag caccgcgttg cccttacccg
     2101 cttgccccta ggtcagcaca ctcagtcgtc cgctgaccag agcgttctca agccctgcct
     2161 gccagttgtc gactttgaca cccagtcggc cactcaaagg aaaacctgca tacagccagc
     2221 tccagcgacc atcgcttacg gtcaattgtt gacgagtaat ttgccacggc aaatcgagca
     2281 acggatcgcc gttatcccgt gccagcacga tcaattgccc gctattttcc tgccagtcca
     2341 gctcggcatc caccagtgac ggttcctgcg gcaagtttaa cgtcgcagta gcatgcccac
     2401 ttacaggaag tccatccggc acgagcggca tagtaaattc ccccaccagt ttaaccggcg
     2461 gctgattttc aaacgcgacg acatccagtt cgctgactgt aagttgttgc cctttcagct
     2521 ggccttgaaa tttaactttt tcgccctgat aacgcagttg ctggatatca gaggttaatg
     2581 cgagagagag ttttccctgc cattcctgcc agggagaaaa aatcagttta tcgatattga
     2641 tccaggtgtt aggcagcatg gactgccact gcgcgagggt ttttggagca gctggcgatt
     2701 gctccgtctg cggcaatttc gccagacaag cagaatcaag ttctaccgtg ccgacgttca
     2761 ataaccagcg gctgggatgt gaaaggctgg cgttggtgat atgcgcaagc tgacaatctc
     2821 ccaccagata acggagatcg ggaatgatta aacctttacg cgtaatgcgt gggctttcat
     2881 ctaatgcaat acgtgtcccg agcggtagcc agatgcccgc cagcgtggga acccacagcc
     2941 cgagcgtcat cagcagcgtc aacggcacaa gaataatcag taataacagc gcgagaacgg
     3001 ctttatattt acccagcatg ggtagttaac atcctgattt agcgaaaaat taagcattca
     3061 atacgggtat tgtggcatgt ttaaccgttc agttgaaggt tgcgcctaca ctaagcatag
     3121 ttgttgatga atttttcaat atcgccatgg ctttcaatta tatttgaaat tttgtaaaat
     3181 atttttagta gcttaaatgt gattcaacat cactggagaa agtcttatga aactcgccgt
     3241 ttatagcaca aaacagtacg acaagaagta cctgcaacag gtgaacgagt cctttggctt
     3301 tgagctggaa ttttttgact ttctgctgac ggaaaaaacc gctaaaactg ccaatggctg
     3361 cgaagcggta tgtattttcg taaacgatga cggcagccgc ccggtgctgg aagagctgaa
     3421 aaagcacggc gttaaatata tcgccctgcg ctgtgccggt ttcaataacg tcgaccttga
     3481 tgcggcaaaa gaactggggc tgaaagtagt ccgtgttcca gcctatgatc cagaggccgt
     3541 tgctgaacac gccatcggta tgatgatgac gctgaaccgc cgtattcacc gcgcgtatca
     3601 gcgtacccgt gacgctaact tctctctgga aggtctgacc ggctttacta tgtatggcaa
     3661 aacggcaggc gttatcggta ccggtaaaat cggtgtggcg atgctgcgca ttctgaaagg
     3721 ttttggtatg cgtctgctgg cgttcgatcc gtatccaagt gcagcggcgc tggaactcgg
     3781 tgtggagtat gtcgatctgc caaccctgtt ctctgaatca gacgttatct ctctgcactg
     3841 cccgctgaca ccggaaaact accatctgtt gaacgaagcc gccttcgatc agatgaaaaa
     3901 tggcgtgatg atcgtcaata ccagtcgcgg tgcattgatt gattctcagg cggcaattga
     3961 agcgctgaaa aatcagaaaa ttggttcgtt gggtatggac gtctatgaga acgaacgcga
     4021 tctattcttt gaagataaat ccaacgacgt gatccaggat gacgtattcc gtcgcctgtc
     4081 tgcctgccac aacgtgctgt ttaccgggca ccaggcattc ctgacagcag aagctctgac
     4141 cagtatttct cagactacgc tgcaaaactt aagcaatctg gaaaaaggtg aaacctgccc
     4201 gaacgaactg gtttaatctt gccgctcccc tgcaatccag gggagctgat tcagataatc
     4261 cccaatgacc tttcattctc tattcttaaa atagtcctga gtcagaaact gtaattgaga
     4321 accacaatga agaaagtagc cgcgtttgtt gcgctaagcc tgctgatggc gggatgtgta
     4381 agtaatgaca aaattgctgt tacgccagaa cagctacagc atcatcgctt tgtgctggaa
     4441 agcgtaaacg gtaagcccgt gaccagcgat aaaaatccgc cagaaatcag ctttggtgaa
     4501 aaaatgatga tttccggcag catgtgtaac cgctttagcg gtgaaggcaa actgtctaat
     4561 ggtgaactga cagccaaagg gctggcaatg acccgtatga tgtgcgctaa cccgcagctt
     4621 aatgaactcg ataacaccat tagcgaaatg ctgaaagaag gtgcacaagt ggatctgacc
     4681 gcgaaccagt taacgctggc gaccgcgaaa cagacattaa cttataagct ggcggattta
     4741 atgaattaat agctgccaca gctcccggcg gcaagtgact gttcgctaca gcgtttgccg
     4801 ttgggtaatg cacacatccc aatcgccgta ccatccagtt gacgggcaac agaaagcgaa
     4861 ccgccgatca ttgcacaatt tgcttctcca ctactggaca tcgacgcttt taaacctggc
     4921 gctacgtgcg ccgcagtcgc ctgctgaaca ggctcactac tacacgccga caacaataaa
     4981 gcggcacacc ctacccaaaa cgctgctcgc atctcttctt cctctgatct tcaagccaaa
     5041 cgacaccgcc ataaataata ggcagcacag agggcggcgt cgagagctgt cctgcgcgtt
     5101 gccccgcatt tttacttttt tatggctatt tttttgccct ctgtttgatc aaaacattca
     5161 ttactctgat gtgggggaca caaaagcgaa aatgcagaag aaagccattt gctaaaattg
     5221 aaggattact actgggcgcg cagcaatttc gtgcgcccct catttgcgca atgtaagggt
     5281 gtcatatgat tactattgac ggtaatggcg cggttgcttc ggtggcgttt cgcaccagtg
     5341 aagttatcgc catctaccct attaccccca gttccacgat ggcagaacag gctgatgcct
     5401 gggccggaaa cggcttgaag aacgtttggg gagacacacc acgcgtggtt gaaatgcagt
     5461 cggaagcggg tgctatcgct accgtgcatg gcgctttgca gacgggtgcc ctttcaacat
     5521 cgtttacgtc atcgcagggt ttgctgctga tgatcccgac gatgtacaaa ctggcaggcg
     5581 aactgacgcc atttgtcctg catgtagcgg cacgtaccgt tgccacacat gcactttcta
     5641 tttttggcga tcattccgac gttatggcgg tgcgccagac gggttgcgcg atgttgtgtg
     5701 cggctaacgt ccaggaagcg caagactttg ctctcatttc gcacatcgca acgctgaaga
     5761 gccgcgtgcc atttattcat ttctttgatg gtttccgcac gtcccacgaa atcaataaaa
     5821 ttgtcccgct ggccgatgac actattcttg atctcatgcc gcaggccgaa attgatgctc
     5881 atcgcgcccg ggcgctcaac ccggaacatc cggtgatccg cggtacgtcc gccaatcctg
     5941 acacttattt ccagtctcgc gaagccacca acccctggta caacgcggtc tatgaccatg
     6001 ttgaacaggc gatgaatgat ttcgctgccg cgacaggtcg tcagtatcag ccgtttgagt
     6061 attacgggca tccgcaagcg gaacgggtga ttatcctgat gggctctgcc attggcacct
     6121 gtgaagaagt ggttgatgaa ttgctaaccc gtggcgaaaa agttggcgtg ctgaaagttc
     6181 gtctgtaccg tcccttctcc gccaaacact tgctgcaagc tctgccggga tccgtacgca
     6241 gcgtggcggt actggacaga accaaagaac ccggtgccca ggcagaaccg ctctatctgg
     6301 atgtgatgac cgcactggca gaagccttta ataatggtga gcgcgaaacc ctgccccgtg
     6361 tcattggtgg gcgctatggt ctttcatcca aagaatttgg cccggactgt gtactggcgg
     6421 tatttgccga gctcaacgcg gctaaaccta aagcacgctt tacggttggt atttacgatg
     6481 atgtgaccaa tctgtcactg ccgttgccgg aaaacactct gccaaactcg gcgaaacttg
     6541 aagccttgtt ttatggcctt ggtagtgatg gcagcgtttc cgcaaccaaa aacaatatca
     6601 agattatcgg taattccacg ccgtggtacg cacagggcta ttttgtttac gactccaaaa
     6661 aagcgggcgg cctgacggtt tctcaccttc gcgtgagcga acagccgatt cgttccgctt
     6721 atctcatttc ctaggctgat tttgttggct gccaccagtt gcagtttatc gataaatatc
     6781 agatggctga gcgtttaaaa cctggcggca ttttcctgct taatacgccg tacagcgcag
     6841 atgaagtgtg gtcgcgcttg ccgcaggaag ttcaggccgt gttaaaccag aaaaaagcgc
     6901 gcttctatgt gattaacgcg gcgaaaatcg cccgcgaatg tggcctggcg gcccgtatta
     6961 ataccgtcat gcagatggct tttttccatc tgacgcaaat tctgcctggc gatagcgccc
     7021 tcgcagaatt gcagggtgcg attgccaaaa gttacagtag caaaggtcag gatctggtgg
     7081 agcgcaactg gcaggctctg gcgctggcgc gtgaatccgt agaagaagtt ccgttgcaac
     7141 cggtaaatcc gcacagcgcc aatcgaccgc cagtggtttc cgatgccgcc cctgatttcg
     7201 tgaaaaccgt aaccgcggcg atgctcgccg ggcttggtga tgccctcccc gtttcggcgc
     7261 tgccgccaga cggcacctgg ccgatgggca ccactcgctg ggaaaaacgc aatatcgccg
     7321 aagagatccc catctggaaa gaggaactct gtacccaatg taaccactgc gtcgccgctt
     7381 gcccacactc agctattcgc gcaaaagtgg taccgcctga agcgatggaa aacgcccctg
     7441 ccagcctgca ttcgctagat gtgaaatcgc gtgatatgcg cgggcagaaa tatgtcttgc
     7501 aggtggcacc ggaagattgc accggttgta acctgtgcgt cgaagtttgc ccggcgaaag
     7561 accgtcagaa tccagagatt aaagccatca atatgatgtc tcgcctggaa catgtcgaag
     7621 aagagaaaat caattacgat ttcttcctca acctgccaga aatcgaccgt agcaaactgg
     7681 aacgtattga tattcgtaca tcgcagctga ttacaccgct gtttgaatat tcaggtgctt
     7741 gctccggttg tggcgagacg ccgtatatta aattgctgac tcagctctat ggtgaccgga
     7801 tgttgatcgc taacgccacc ggctgttctt caatttatgg cggtaacctg ccctctacac
     7861 cgtataccac cgatgccaac ggtcgtgggc cggcatgggc gaactctcta tttgaagata
     7921 atgccgaatt tggccttggt ttccgcctga cggtcgatca acaccgtgtc cgcgtgctgc
     7981 gtctgctgga tcaatttgcc gataaaatcc cggcggaatt actgacggcg ttgaaatcag
     8041 acgctacgcc agaggttcgt cgagaacagg tcgcagcttt acgccagcaa ctcaacgatg
     8101 ttgccgaagc acatgaactg ctgcgtgacg cagatgcact ggtggaaaaa tcaatctggc
     8161 tgattggtgg cgacggctgg gcttacgata tcggctttgg cggtctggat catgtattga
     8221 gtttgacgga aaacgtcaac attctggtgc tggatacgca atgctattcc aacaccggtg
     8281 gtcaggcgtc gaaagcgaca ccgctgggtg cagtaactaa gttcggcgag cacggcaaac
     8341 gtaaagatct tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg cagatttctc
     8401 tcggcgcgca gctgaaccag acggtgaaag cgattcagga agcggaagcg tatccgggac
     8461 catcgctgat cattgcttat agcccgtgtg aagagcatgg ttacgatctg gctctcagcc
     8521 acgaccagat gcgccaactc actgctaccg gcttctggcc gctatatcgc tttgatccgc
     8581 gtcgtgccga tgaaggcaaa ctgccgctgg cgctggattc acgcccgccg tcagaagcac
     8641 tggaagaaac gttacttcac gagcagcgtt tccgtcgtct gaattcgcag cagccagaag
     8701 tggcagaaca gttatggaaa gatgctgcag ctgatttgca aaaacgctat gacttcctgg
     8761 cacaaatggc cggaaaagcg gaaaaaagca acaccgatta aatgctctgg ataaggatta
     8821 tccaattcta aaaaaaaagc ccggacgact gttcgggctt gtctttttat atgttggaaa
     8881 atcagtggca atgcaatggc ccaacagaaa ataaaatctg tgacaataaa ggcatataac
     8941 ccgcgcagaa taacgtatag aaaattaaaa gtataatttt tattttgtat gtattatctt
     9001 cgatacattc accagactta ttatcatttc agaggaatta tttgattaag gttttactta
     9061 aggcgtaaca aatgatattt atcggctaac tgaacttctc ctttaggatg ttttcactcc
     9121 ccgaaatggg gataaaaggc aaataaaata actaaaggat ttattcaatg aaaagaaaag
     9181 tactggcact tttaattcct gccctgctcg ccgcaggtgc tgcacatgca gccgaagttt
     9241 ataataaaga cggcaacaaa ttagatctgt atggcaaagt tgatggcctg cattattttt
     9301 ctgataattc agcgaaagat ggcgaccaga gctatgcgcg tctgggtttt aaaggcgaaa
     9361 cccaaattaa cgatcaactt actggctacg gtcaatggga atacaatatt caggcaaaca
     9421 acactgaatc ttcaaaaaac cagtcatgga cccgtctggc atttgccgga ctgaaatttg
     9481 cagattacgg ttctttcgat tacggacgta attatggcgt aatgtacgac atcgaaggct
     9541 ggaccgatat gctgcctgaa tttggcggtg actcttatac caatgcagac aactttatga
     9601 ctggtcgagc caatggcgtc gcgacttatc gtaatactga tttcttcggt ctggtaaatg
     9661 gtctgaactt cgcggtgcag tatcaaggta acaacgaagg agccagtaat ggtcaggaag
     9721 gcaccaataa cgaacgcgat gttcgtcatg aaaacggtga cggctggggt ctttccacaa
     9781 catatgattt aggcatgggc tttagcgctg gtgcggcata cacctcttct gaccgcacca
     9841 atgaccaggt taaccatact gcggcgggtg gtgataaagc agacgcgtgg actgctgggc
     9901 taaaatacga tgctaacaat atttacctag caaccatgta ttcagaaacg cgtaatatga
     9961 ccccgtttgg cgacagcgat tatgtagttg cgaacaaaac ccagaacttt gaa
//
LOCUS       DADLSA010000138         9873 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.139, whole genome shotgun sequence.
ACCESSION   DADLSA010000138 DADLSA010000000
VERSION     DADLSA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.139"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..518
                     /locus_tag="JN691_003305"
     CDS             <1..518
                     /locus_tag="JN691_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAY7244663.1"
                     /translation="SILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVKAT
                     GSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSWLVQPVRSEET
                     LHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYKSA
                     ESVVQCLGRPD"
     gene            634..1194
                     /locus_tag="JN691_003306"
                     /pseudo
     CDS             634..1194
                     /locus_tag="JN691_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1251..2708)
                     /gene="pepD"
                     /locus_tag="JN691_003307"
     CDS             complement(1251..2708)
                     /gene="pepD"
                     /locus_tag="JN691_003307"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY7244664.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2970..3428
                     /gene="gpt"
                     /locus_tag="JN691_003308"
     CDS             2970..3428
                     /gene="gpt"
                     /locus_tag="JN691_003308"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY7244665.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3520..4764
                     /gene="frsA"
                     /locus_tag="JN691_003309"
     CDS             3520..4764
                     /gene="frsA"
                     /locus_tag="JN691_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY7244666.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4822..5223
                     /locus_tag="JN691_003310"
     CDS             4822..5223
                     /locus_tag="JN691_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY7244667.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5262..6317)
                     /gene="phoE"
                     /locus_tag="JN691_003311"
     CDS             complement(5262..6317)
                     /gene="phoE"
                     /locus_tag="JN691_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY7244668.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            6605..7708
                     /gene="proB"
                     /locus_tag="JN691_003312"
     CDS             6605..7708
                     /gene="proB"
                     /locus_tag="JN691_003312"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY7244669.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7720..8973
                     /gene="proA"
                     /locus_tag="JN691_003313"
     CDS             7720..8973
                     /gene="proA"
                     /locus_tag="JN691_003313"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY7244670.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIVEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9088..9163
                     /locus_tag="JN691_003314"
     tRNA            9088..9163
                     /locus_tag="JN691_003314"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9121..9123,aa:Thr,seq:cgt)
     gene            complement(9178..>9873)
                     /locus_tag="JN691_003315"
     CDS             complement(9178..>9873)
                     /locus_tag="JN691_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7244671.1"
                     /translation="LRAAEREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPE
                     PLKSVVEFALATGLRRSNIINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRV
                     LKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDL
                     RHTWASWLVQAGVPLSVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPN
                     LSQSKNKEGTNDV"
BASE COUNT         2363 a         2457 c         2589 g         2464 t
ORIGIN      
        1 agtctatttt acagcggcat attgcctcgt tttcgcatca tggtttgcct gaaggcagtg
       61 acgacgcgct ggcgtttgcg cgttttaatc gccacctgat cgctttgcgc ataatgcaac
      121 aggttaaggc caccggaagt ccggttctgg atgtggcgca taactttgtt agcgcgtgtc
      181 gaatcggtga tcaacaaggg gtcttacatc gtaaaggtgc cacgcctgat gactgtggtc
      241 tggtggttat tcccggttca cgcggtgatt actcctggct ggttcagccc gtcaggagtg
      301 aggaaacatt gcattcgctg gcgcatgggg ctgggcgtaa atgggggcgc accgagtgta
      361 aagggcgtct ggcagcgaaa tacacagcga cgcaactctc gcgtactgaa cttggcagtc
      421 gggtaatttg tcgcgataaa caactcatct tcgaagaagc gccacaagcc tataaatcgg
      481 ctgaaagtgt ggtgcaatgt ctgggcaggc cggattaatt attcctgtcg cgcggctgcg
      541 tccggtgcta acgctcaaaa acagtggagg gaaaaaagga tgatcttgct ataactctcc
      601 tcagcgcagg ggccggaaga atgttgtctc gcagtgaaaa aggcgctgga caggctaatt
      661 aaagaagctg cccgacaaga cgtcgcggta acggtgctgg aaacagaaac aggccgctac
      721 tctgacacgc tgcgttcggc gctgatttct ctggatggcg acaacgcatg ggcgttaagc
      781 gaaagctggt gtggcactat tcagtggatt tgcccgagcc cgtatcgccc acatcacggg
      841 cgtaaaaact ggtttctggg cattgggcgt tttaccgctg atgagcagga gcaatccgat
      901 gcgatccggt atgagacgct gcgttcgtcg gggccgggcg gtcaacatgt caataaaacc
      961 gactcggcgg tacgcgccac gcatttggca tccggtatta gcgtgaaggt tcagtcagag
     1021 cgcagccagc atgctaacaa acggctggcg cgattgctga ttgcctggaa gctggatcag
     1081 cagcaatagg aaaatagcgc ggtgctgaaa tcacagcggc gaatgttcca tcaccagatt
     1141 gaacgtggca acccgcgacg aacgtttaca gggatggctt ttatcgaagg ataatgaagg
     1201 atgaaactgc cggaaggcga ttaaacgcca tccggcagca aatcaaataa ttacttcgcc
     1261 ggaatttctt tcagcagttc agtcagcagt gtccagtaat gacctacgct ttcgatgtga
     1321 acttgctcat ccggagagtg tggaccggtg atagttggcc cgatagaaac catgtccatt
     1381 tccggatacg gttttttgaa cagaccacat tccaggcccg cgtggataat ctggatgttc
     1441 ggcgtcttgt tgaacaggcg ctgataggtt tcacgtacca gatgcatcac cggagaatta
     1501 gcgtccggct gccagccagg atatgcgcct ttcgcttcgg ttttcgcgcc agccagttta
     1561 cccagcgaat ccagcatgct caccacgtag tctttaccgc tgtcgatcag tgaacggatc
     1621 aggcagtgaa tttctacgtt attgtcagtc atggtcacca caccgacgtt cagggaggtt
     1681 tcgaccacac cttttgccac gtcggaattg cggatcacac cgttcggggt ggcgttcagc
     1741 agacgaataa aggtatcgcg agatttcgca atcagggcag ctttatcgtt cgctacagag
     1801 tccagcaaca aggccagatt tttctctttc tctgccagct cgtttttcag gatctcctga
     1861 taggtattca ccagagattt cagtgcgtcg actttatcag ctgcgacagc aatggtcgca
     1921 aaggcttcac gcgggatggc gttacgcagt gtgccgccgt tgaaatcgat aaggcgcagg
     1981 tccagttctt ctgcatgacc cgccaggaag cgcaccagca gtttgttggc attacccagg
     2041 ccaacgtgga tttcaccgcc ggagtgaccg cctttcagac cttttaaggt taacttgaag
     2101 gtttcaaaac cagctggaac cgcttcacga tctaaatgca ggttggaggt gaagtcgata
     2161 ccccccgcac aacccatgta gatttcacct tcttcttcgg agtcggtgtt aatcagaata
     2221 tcagcctgca accagttgct ctgtaagccg aacgcaccgt ccataccggc ttcttcggtc
     2281 atggtcagca gcacttccag cgggccgtga accacgtttt cgtcagccag aaccgccagt
     2341 gcagaggcca taccaatgcc gttatccgca cccagcgtgg tgccgcgcgc tttaacccat
     2401 tcgccatcaa tataaggctg gataggatct ttcgtgaagt catgcacggt gtcgttattt
     2461 ttctgcggca ccatatcgag gtgggcctgt aagacgaccg gtttacgatt ttccatacct
     2521 gcggtagcag gtttacgaat caggatatta cctacctgat cgcgttcgac atggaaacct
     2581 ttctcttttg cccaaccaac aatgtattca gcgagttgct cttcatgata ggacgggtga
     2641 ggaatagaac agattttggc aaaaatatcc cacagcggct gtggagataa ttgagacagt
     2701 tcagacacgt taagtctcct tgtcgatcac ccgcaaaaca gtattgcagg tcacagggtt
     2761 agcagaaaat gttgtcaaca caagacaggc ttgcgagata tgtattgaga ataccacttt
     2821 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
     2881 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
     2941 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     3001 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     3061 gcattattgc cgtaagccgt ggcggtctgg taccgggtgc gttactggcg cgtgaactgg
     3121 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
     3181 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
     3241 tggataccgg tggtactgct gttgcgattc gtgaaatgta tccaaaagcg cactttgtca
     3301 ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc
     3361 aagatacctg gatcgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg
     3421 gtcgctaatc ttttcaacgc ctggcattgc cgggcgttgt tctttttaac ttcaggcagg
     3481 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc
     3541 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt
     3601 aatcacggcg cacaaccgcc tgtgcagtcg gctcttgatg gtaaaaccat ccctcactgg
     3661 tatcgcatga ttaaccgtct gatgtggatc tggcgcggta ttgacccacg tgaaatcctc
     3721 gacgtccagg cacgtattgt gatgagcgat gctgaacgta ccgacgatga tttatacgat
     3781 accgtgattg gctaccgtgg tggcaactgg atttatgagt gggccaccca ggcgatggtg
     3841 tggcaacaaa aagcctgtac ggaagaagat ccgcaactca gtggtcgaca ctggctgcat
     3901 gcggctacgt tgtacaacat tgccgcctat cctcatctga aaggagatga cctggccgag
     3961 caagcgcagg ctttatcaaa ccgcgcctat gaagaggccg cccagcgtct gccgggcacg
     4021 atgcggcaga tggagtttac cgtacccggc ggtgcgccca tcaccggctt tttgcatatg
     4081 ccgaaaggcg atggcccgtt cccgacagta ttaatgtgtg gtggtctgga tgcgatgcag
     4141 acggactatt acagcctgta tgaacgttat tttgcgccgc gcggcattgc gatgctgact
     4201 attgatatgc cgtcggtggg cttttcttca aaatggaagc tcacccagga ctccagcctg
     4261 ttgcatcagc acgtcttaaa ggcgctgcct aacgtaccgt gggtggatca cactcgcgtc
     4321 gcggcctttg gtttccgttt cggcgctaac gttgccgtgc gtctggcata ccttgaatcg
     4381 ccgcgtctga aagcggttgc ctgtcttggt ccggtagttc ataccctgtt gagtgatttt
     4441 aagtgccagc aacaggtgcc ggaaatgtat cttgacgttc tggcgagtcg tttggggatg
     4501 catgatgctt ccgacgaagc gttacgcgtg gagctgaatc gctattcatt aaaagtgcaa
     4561 ggattgctgg ggcgtcgctg cccaacgcca atgttatcag gctactggaa gaacgatccg
     4621 ttcagcccgg aagaggactc acgcttaatc acctcatcat ctgctgacgg taaattatta
     4681 gagatcccat ttaacccggt gtatcggaat tttgacaaag gtcttcagga aatcaccggc
     4741 tggatcgaaa aacgcttgtg ttaaaaattt gctaaatttt gccaatttgg taaaacagtt
     4801 gcatcacaac aggagatagc aatgacgtta ccgagtggac acccgaagag cagattgatc
     4861 aaaaaattta ccgcactagg cccgtatatt cgtgaaggta agtgcgaaga taatcgattc
     4921 tttttcgatt gtctggctgt atgcgtcaac gtgaaaccgg caccggaagt gcgtgaattc
     4981 tggggctggt ggatggagct ggaagcgcag gaatcccgtt ttacctacag ctaccagttt
     5041 ggtctgttcg ataaagcagg cgactggaag agtgttccgg taaaagatac tgaagtggtt
     5101 gaacgactgg agcacaccct gcgtgagttt cacgagaagc tgcgtgaact gctgacgacg
     5161 ctgaatctga agctggaacc ggcggatgat tttcgtgatg aaccggtgaa gttaacggcg
     5221 tgagtgaaat gtgccggatg catcacatcc ggcaatattc attaaaactg atacgtcatc
     5281 ccaaccgcga cagtatcatc attattaata ttaagcttat tatcgctatc cagttggttg
     5341 attttataat caacaaacgc tgacatattt ttgttgaaat aatacgtagc accgacgtcg
     5401 atataattga ccagatcttc atcaccgatg ccctcaatat ctttcccttt cgataagaca
     5461 taacccagcg atggacgcag accaaagtca aactggtatt gagcgaccgc ttcaaagttc
     5521 tgtgtcttat tggcaaagcc gccagttatt ggcgtcattt tgcgtgtttc agaatagaaa
     5581 gttgccagat aaatattatt ggcatcgtat ttcagacctg ttgcccatgc ttctgcacgc
     5641 ttgcctgtgc cacggctttg caggttctgc tcgttggtgc gatctgagtt ggtataggcc
     5701 ccactaatgg cgaaatcgct gccgccaaag tcatatgtca atgacgtgcc gaagccatcg
     5761 ccgttttgct ttttaacgtc gcggttttcg tttttccctt gatattgcag ggttaagttc
     5821 aggccatcga taacgccgaa gaagtcggtg ttccgatacg tcgccagacc gctggcgcgt
     5881 ttggtcataa agttgtcggt ctgcgccgag gaatcgccac caaattccgg gaacatatcg
     5941 gtccaggctt ccacgtcata caacgcgccc aggttacgac cataatcgaa agaacccaaa
     6001 tctttatatt tcaacccggc aaaagcgaga cgcgtttttt gctgtgcagt atcactctcc
     6061 gctttatttc cggcaaactc cgcttcccac cgaccataac cagtcagttg atcgttaatt
     6121 tgagtttcac ctttaaaacc aaaacggata taactctggt cgccatcttt actggcgtta
     6181 tcactcatat aatgcatggc tttaactttg ccatagacat ccagtttatt accgtcttta
     6241 ttatatattt ctgcagcctg tacagatgca gatgccacaa tgcccatcac cactaatgcc
     6301 agagtgctct ttttcatttt cattcctgat tttaattaac gcgcgaatat tcagcgggag
     6361 agccccgttg aaaacaggaa agtttttaac ctgagattgt taaagatata ttacagatta
     6421 atgatattct gaaaatgtgg taatttatta aatctgtaat aaaagcgtaa acaactgccg
     6481 ctacgcttgc tgatcccgcg caacaaaacg ccatgctttg ctcgcagatg gttggcaacc
     6541 gacgacagtc ctgctaaaac gttcgtttga tatcattttt cctaaaattg aatggcagag
     6601 aatcatgagt gacagccaga cgctggtggt aaaactcggc accagtgtgc taacaggcgg
     6661 atcgcgccgt ctgaaccgtg cccatatcgt tgaacttgtt cgccagtgcg cgcagttaca
     6721 tgccgccggg catcggattg ttattgtgac gtcgggcgcg atcgccgccg gacgtgagca
     6781 cctgggttac ccggaactgc cagcgactat cgcctcgaaa caactgctgg cggcggtagg
     6841 gcagagtcga ctgattcaac tgtgggaaca gctgttttcg atttatggca ttcacgtcgg
     6901 gcaaatgctg ctgacccgtg ctgatatgga agaccgtgaa cgcttcctga acgcccgcga
     6961 caccctgcgt gcgttgctcg ataacaatat cgttccggta atcaatgaga acgatgctgt
     7021 cgctacggca gagattaagg tcggcgataa cgataacctt tctgcgctgg cggcgattct
     7081 ggcgggtgcc gataaactgt tgctgctgac cgatcaaaaa ggtttgtata ccgctgatcc
     7141 gcgcagcaat ccgcaggcag aactgattaa agatgtttac ggcattgatg acgcactgcg
     7201 cgcgattgcc ggtgacagcg tttcaggcct cggaactggc ggcatgagta ccaaattgca
     7261 ggccgctgac gtggcttgcc gtgcgggtat cgacaccatt attgccgcgg gcagcaagcc
     7321 gggcgttatt ggtgatgtga tggaaggcat ttccgtcggt acgctgttcc atgcccaggc
     7381 gactccgctt gaaaaccgta aacgctggat tttcggtgcg ccgccggcgg gtgaaatcac
     7441 ggtagatgaa ggggcaactg ccgccattct ggaacgcggc agctccctgt tgccgaaagg
     7501 cattaaaagc gtgactggca atttctcgcg tggtgaagtc atccgcattt gcaacctcga
     7561 aggtcgcgat atcgcccacg gtgtcagtcg ttacaacagc gatgcattac gccgtattgc
     7621 cggacaccac tcgcaagaaa ttgatgcaat actgggatat gaatacggcc cggttgccgt
     7681 tcaccgtgat gacatgatta cccgttaagg agctggccga tgctggaaca aatgggcatt
     7741 gccgcgaagc aagcctcgta taaattagcg caactctcca gccgcgaaaa aaatcgcgtg
     7801 ctggaaaaaa tcgccgatga actggaagca caaagcgaaa tcatcctcaa cgctaacgcc
     7861 caggatgttg ctgacgcgcg agccaatggc cttagcgaag cgatgcttga ccgtctggca
     7921 ctgacgcccg cacggctgaa aggcattgcc gacgatgtac gtcaggtgtg caacctcgcc
     7981 gatccggtgg ggcaggtaat cgatggcggc gtactggaca gcggcctgcg tcttgagcgt
     8041 cgtcgcgtac cgctgggggt tattggcgtg atttatgaag cgcgcccgaa cgtgacggtt
     8101 gatgtcgctt cgctgtgcct gaaaaccggt aatgcggtga tcctgcgtgg tggtaaagaa
     8161 acctgtcgca ctaacgcggc aacggtggcg gtgattcagg acgccctgaa atcctgtggc
     8221 ttaccggcgg gtgccgtgca ggcgattgat aatcctgacc gtgcgctggt cagtgaaatg
     8281 ctgcgtatgg ataaatacat cgacatgctg atcccgcgtg gtggcgctgg tttgcataaa
     8341 ctgtgccgtg aacagtcgac aatcccggtg atcacaggtg gtataggcgt atgccatatt
     8401 tacgttgatg aaagtgcaga gatcgttgaa gcattaaaag tgatcgtcaa tgcgaaaact
     8461 cagcgtccga gcacatgtaa tacggttgaa acgttgctgg tgaataaaaa catcgccgat
     8521 agcttcctgc ccgcattaag caaacaaatg gcggaaagcg gcgtgacatt acacgcagat
     8581 gcagctgcgc tgacacagtt gcaggcaggc cctgcgaagg tggtggcggt taaagccgaa
     8641 gagtatgacg atgagtttct gtcattagat ttgaacgtca aaatcgtcag cgatcttgac
     8701 gatgccatcg cccatattcg tgaacacggc acacaacact ccgatgcgat cctgacccgc
     8761 gatatgcgca acgcccagcg ttttgttaac gaagtggatt cgtccgctgt ttacgttaac
     8821 gcctctacgc gttttaccga cggcggccag tttggtctgg gtgcggaagt ggcggtaagc
     8881 acacaaaaac tccacgcgcg tggcccaatg gggctggaag cactgaccac ttacaagtgg
     8941 atcggcattg gtgattacac cattcgtgcg taaataaaac cgggtgatgc aaaagtagcc
     9001 atttgattca caaggccatt gacgcatcgc ccggttagtt ttaaccttgt ccaccgtgat
     9061 tcacgttcgt gaacatgtcc tttcagggcc gatatagctc agttggtaga gcagcgcatt
     9121 cgtaatgcga aggtcgtagg ttcgactcct attatcggca ccattaaaat caataagtta
     9181 cacatcatta gtaccttcct tattttttga ctgggacaaa tttgggaccg atgggttcag
     9241 gatcgagtct atttgccgtg cgtgttcggt aaggtgatta ggtgcaaggt gagcatatcg
     9301 acgaaccatt tcgatagact cccagcctcc catttcctgt aacactgaca acgggactcc
     9361 ggcttgaacc agccaacttg cccaggtgtg tctcaagtcg tgaaatctga aatcatcaat
     9421 accagcccgt ctcagcgccg ctttccaggc tgtgtttgcg tcataccgca tcttccttac
     9481 tgttggcgct ttcgttccgt ctggtttggt acagctttcc ttgtacacaa atacccaacg
     9541 gtgatgattc ccgatttgtt ttttcaaaac gcgacatgca gtatcattca gcgcaacgcc
     9601 gattgcgcgg tttgatttac tctcttccgg gtttatccat gccacccggc gctgcatatc
     9661 tatttgttgc cattcaaggt tgatgatgtt cgagcgtctt aagcctgttg ccagtgcaaa
     9721 ttcaacaaca gactttaatg gctccggaca ttcatcaatc agcctttgtg cttcatgggg
     9781 ctccagccag cggatccgtt tattctttgg ttgaggcact ttaataattg gtgccttatc
     9841 cagcattttc cattcacgct ctgcggctct tag
//
LOCUS       DADLSA010000139         9860 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.140, whole genome shotgun sequence.
ACCESSION   DADLSA010000139 DADLSA010000000
VERSION     DADLSA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.140"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            374..1495
                     /gene="ompC"
                     /locus_tag="JN691_003316"
     CDS             374..1495
                     /gene="ompC"
                     /locus_tag="JN691_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY7244672.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1607..2662
                     /gene="apbE"
                     /locus_tag="JN691_003317"
     CDS             1607..2662
                     /gene="apbE"
                     /locus_tag="JN691_003317"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAY7244673.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2736..3800
                     /gene="ada"
                     /locus_tag="JN691_003318"
     CDS             2736..3800
                     /gene="ada"
                     /locus_tag="JN691_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAY7244674.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3800..4450
                     /gene="alkB"
                     /locus_tag="JN691_003319"
     CDS             3800..4450
                     /gene="alkB"
                     /locus_tag="JN691_003319"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HAY7244675.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     EATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4526..6169
                     /gene="yojI"
                     /locus_tag="JN691_003320"
     CDS             4526..6169
                     /gene="yojI"
                     /locus_tag="JN691_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAY7244676.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6387..8033
                     /gene="mqo"
                     /locus_tag="JN691_003321"
     CDS             6387..8033
                     /gene="mqo"
                     /locus_tag="JN691_003321"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HAY7244677.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGGAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNRHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGALWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8183..8671)
                     /gene="eco"
                     /locus_tag="JN691_003322"
     CDS             complement(8183..8671)
                     /gene="eco"
                     /locus_tag="JN691_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAY7244678.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(8756..8971)
                     /locus_tag="JN691_003323"
     CDS             complement(8756..8971)
                     /locus_tag="JN691_003323"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244679.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAIIFTRD
                     ISAGLLPHQNLTLKEQVKLSECSLSYS"
     gene            9080..9574
                     /gene="napF"
                     /locus_tag="JN691_003324"
     CDS             9080..9574
                     /gene="napF"
                     /locus_tag="JN691_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAY7244680.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9564..9827
                     /gene="napD"
                     /locus_tag="JN691_003325"
     CDS             9564..9827
                     /gene="napD"
                     /locus_tag="JN691_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAY7244681.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQQNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
BASE COUNT         2397 a         2425 c         2631 g         2407 t
ORIGIN      
        1 aaatgaatga cggtaataaa taaagttaat gatgatagcg ggagttattc tagttgcgag
       61 tgaaggtttt gttttgacat tcagtgctgt caaatactta agaataagtt attgatttta
      121 accttgaatt attattgctt gatgttaggt gcttatttcg ccattccgca ataatcttaa
      181 aaagttcact tgcatttaca ttttgaaaca tctatagcga taaatgaaac atcttaaaag
      241 ttttagtatc atattcgtgt tggattattc tgcatttttg gggagaatgg acttgccgac
      301 tgattaatga gggttaatca gtatgcagtg gcataaaaaa gcaaataaag gcatataaca
      361 gagggttaat aacatgaaag ttaaagtact gtccctcctg gtcccagctc tgctggtagc
      421 aggcgcagca aacgctgctg aagtttacaa caaagacggc aacaaattag atctgtacgg
      481 taaagtagac ggcctgcact atttctctga cgacaagtct gtagatggcg accagaccta
      541 catgcgtctt ggcttcaaag gtgaaactca ggttactgac cagctgaccg gttacggcca
      601 gtgggaatat cagatccagg gcaacagcgc tgaaaacgaa aacaactcct ggacccgtgt
      661 ggcattcgca ggtctgaaat tccaggatgt aggttctttc gactacggtc gtaactacgg
      721 cgtagtttac gacgtaactt cctggaccga cgtactgcca gaattcggtg gtgacactta
      781 cggttctgac aacttcatgc agcagcgtgg taacggcttc gcaacctacc gtagcaccga
      841 cttctttggt ctggttgacg gtctgaactt cgctgttcag tatcagggca aaaacggtag
      901 cccggaaggt gaaggcatga ccaacaacgg tcgtgaagct ctgcgtcaga acggtgacgg
      961 cgttggcggt tctattactt atgattacga aggcttcggt atcggtgctg cagtttccag
     1021 ctccaaacgt actgatgacc agaacttcgg tctgaaccgt tatgatgagc gttacatcgg
     1081 taatggcgac cgcgctgaaa cctacaccgg tggtctgaaa tacgacgcga acaacatcta
     1141 cctagctgct cagtacaccc agacctacaa cgcaactcgc gtaggcaact tgggttgggc
     1201 gaacaaagca cagaacttcg aagctgttgc tcagtaccag ttcgacttcg gtctgcgtcc
     1261 gtccctggct tacctgcagt ctaaaggtaa aaacctgggt gtcatcaatg gtcgtaacta
     1321 cgacgacgaa gatatcctga aatatgttga tgttggtgcg acctactact tcaacaaaaa
     1381 catgtccacc tacgttgact acaaaatcaa cctgctggac gacaaccagt tcactcgtga
     1441 cgctggcatc aacactgata acatcgtagc tttgggtctg gtttaccagt tctaatctcg
     1501 atggatatcg aacaaagggc ctgcgggccc ttttttcatt gttttcagcg tacaaacaca
     1561 gttttttggt gtactcttgc gaccgttcgc atgaggataa tcacgtatgg aaataagctt
     1621 tacccgcgtg gcactgctgg ttgccgcgct cttctttgtt ggttgcgatc aaaaaccaca
     1681 acccgccaaa acccacgcta ctgaagttac tgttcttgaa ggcaaaacca tgggtacctt
     1741 ctggcgtgcc agcatcccgg gcattgatgc caaacgcagt gccgaactta aagaaaagat
     1801 tcagacccag ctggacgccg acgatcagct gctttcgacc tataaaaaag attccgcgct
     1861 gatgcgcttt aacgactcgc aaagtttgtc gccgtggccg gtaagtgaag cgatggccga
     1921 tatcgtcacc acctcgctgc gcattggggc aaggaccgat ggtgcgatgg atattaccgt
     1981 cgggccgctg gtgaatctgt ggggctttgg cccggaacaa cagccggttc aaattccgag
     2041 ccaggaacag atcgatgcga tgaaagccaa aaccggctta cagcacttga cggtcatcaa
     2101 tcagtcgcat cagcaatatc tgcaaaaaga cctgccggat ttatatgtcg atctctccac
     2161 cgtcggtgaa ggttatgcgg cggatcacct ggcacgcttg atggagcagg aagggatttc
     2221 ccgctatctg gtgtcggtgg gcggcgcgct gaacagccgt ggtatgaacg gtgaaggcca
     2281 gccgtggcgg gtagcgattc aaaaaccaac cgataaagaa aacgcggttc aggctgtggt
     2341 ggatatcaac ggtcatggga ttagcacctc tggcagctac cgtaactatt acgaactgga
     2401 cggcaaacgt ctttcccatg ttatcgatcc gcaaaccggg cgtcccatcg aacacaatct
     2461 ggtatccgtg acggtgattg ctccgacggc gctggaagcc gatgcctggg atactggctt
     2521 gatggtactc gggccggaga aagccaaaga ggttgttcgc cgggaagggc tggcggttta
     2581 tatgatcacc aaagaaggcg atagctttaa aacctggatg tcaccacagt ttaaaagctt
     2641 ccttgtcagc gaaaaaaatt aaagcgcaag attgttggtt tttgcgtgat ggtgaccggg
     2701 cagactaaag gctatcctta accagggagc tgattatgaa aaacgccaca tgcttaactg
     2761 acgatcaacg ctggcaatct gtcttagccc gcgacccgaa tgccgacggc gaattcgttt
     2821 tcgccgtgcg caccacgggt atcttttgcc gtccgtcttg ccgcgccaga catgccttgc
     2881 gggaaaatgt ctccttctac gcaaatgcca gcgaggcact cgccgccgga tttcgcccct
     2941 gcaaacgttg tcagccagac aaagccaatc cccggcaaca tcggttggat aaaatcaccc
     3001 acgcgtgtcg actgctggaa caggaaacgc ctgtaacgct ggaagcctta gccgaccagg
     3061 tggcgatgag tccatttcat ctacatcggt tgtttaaagc gactaccgga atgacgccta
     3121 aagcctggca acaggcctgg cgcgctcgcc gtttgcgcga atcgctggcg aaaggggaga
     3181 gcgtgacgac gtctattctt aacgccggat tccccgacag cagcagttac taccgcaaag
     3241 ccgatgaaac gctgggcatg acggctaaac aattccgtca cggtggcgaa aatctggcgg
     3301 tgcgttacgc gctggctgat tgtgagctgg gtcgttgcct ggtggcagaa agcgagcggg
     3361 ggatttgcgc gatattgctg ggcgatgatg acgcgacatt aatcagcgag ttgcagcaga
     3421 tgtttcccgc tgccgacaac gcgcctgccg atctgacgtt tcagcaacat gtgcgtgaag
     3481 tgatcgccag cctcaatcaa cgcgatacgc cgctgacgtt accgctggat attcgcggca
     3541 ctgcttttca gcaacaagtc tggcaggcac tgcgcacgat accttgtggt gaaaccgtca
     3601 gttatcagca actggctaac gccatcggca aaccgaaagc ggtacgggcc gttgccagcg
     3661 cctgtgccgc caacaagctg gctatcgtaa taccctgtca tcgagtggtc cgtggtgatg
     3721 gcacactttc cggttaccgc tggggcgtgt cgcgtaaagc gcaactgctg cgccgcgaag
     3781 ctgaaaatga ggagaggtaa tgttggatct gtttgccgat gctgaaccgt ggcaagagcc
     3841 actggcggct ggtgcggtaa ttttacggcg ttttgctttt aatgctgcgg agcaactaat
     3901 ccgcgatatt aatgacgttg ccagccagtc gccgtttcgc cagatggtcg ctccaggcgg
     3961 atataccatg tcggtggcga tgaccaactg tgggcatctg ggctggacga cccatcggca
     4021 aggttatctc tattcgccca ttgatccgca aacaaataaa ccgtggcccg ccatgccaca
     4081 aagctttcat gatttatgtc aacgtgaggc tacggcggcg ggctatccag atttccagcc
     4141 agatgcttgt cttatcaacc gctacgctcc tggcgcgaaa ctgtcgctgc atcaggataa
     4201 agacgaaccg gatctgcgcg cgccaattgt ttctgtttct ctgggcttac ccgcgatttt
     4261 tcaatttggt ggcctgaaac gtaatgatcc gctcaaacgt ttgttgttgg aacatggcga
     4321 tgtggtggta tggggcggtg aatcgcggct gttttatcac ggtattcaac cgttgaaagc
     4381 ggggtttcat ccactcacca ccgactgccg ctacaacctg acattccgtc aggcaggtaa
     4441 aaaagaataa aaataagaat tattattgct gtgcgcgcga agatgtttaa actgcgggct
     4501 gtaattcatt gtccgggttt tctgcatgga acttcttgta cttgtctggc ggcagtatcg
     4561 ctggccattt atcagtgtga tggcgctaag cctcgccagt gcggcattag gcattggctt
     4621 aattgctttt atcaatcagc gccttatcga aacggcggat accagtctgc tggtgttacc
     4681 ggagtttctg ggattattgc tgctgttgat ggcagtcact ctcggatcgc aactggcgct
     4741 caccactttg gggcatcact tcgtttaccg actgcgtagt gaatttatca agcggattct
     4801 ggatacccac gtcgagcgca ttgaacaact cggtagcgcc tcgttgctgg cgggattaac
     4861 cagcgatgtg cgcaatatca ccattgcttt tgtgcgtctg ccggaactgg ggcaggggat
     4921 cattctcact atcggttcat cggcgtatct gtggatgctg tcgggcaaaa tgttgctggt
     4981 aacggctatc tggatggcga tcaccatctg gggcggtttt gtgctggtgg cgcgggtgta
     5041 caaacatatg gcgaccctac gtgaaaccga agacaagctg tacacggatt ttcaaactgt
     5101 acttgaaggg cgcaaagagc tgactctgaa ccgggaacgc gccgagtatg tgtttaacaa
     5161 cctctacatc cccgatgcgc aagagtatcg ccatcatatt attcgcgcag acaccttcca
     5221 tcttagtgcc gtgaactggt caaacatcat gatgctgggc gctatcggcc tggtgttctg
     5281 gatggcgaac agtctcggtt gggctgatac caacgttgct gcgacctatt cgttgacgct
     5341 gttattcctg cgtacgccgc tgctttcggc ggttggcgca ttgccgacgc tgctgacggc
     5401 gcaggtggcg tttaacaagc tgaacaaatt cgcgctcgcg cctttcaaag ctgagtttcc
     5461 gcgcccgcag gcgtttccca actggcaaac gctggagctg cgtaacgtga cgtttgctta
     5521 tcaggataac gcgttttccg ttggtccgat taatctcacc atcaaacgtg gcgagctgct
     5581 gtttctgatt ggcggcaacg gtagcggaaa atcgacgctg gcgatgttgt tgacgggctt
     5641 gtatcagcca caaagcggcg aaatcttgct ggatggcaaa cctgtcagcg gcgaacaacc
     5701 ggaagattat cgcaaactgt tttcggcagt gtttaccgat gtctggctgt ttgatcaact
     5761 gctggggccg gaggggcaac ccgctaaccc gcaactggtt gagaagtggc tggcgcagct
     5821 gaaaatggct cataagcttg agttaagcaa cgggcgtatt gttaacctga agttatcaaa
     5881 agggcagaaa aaacgcgtgg cgctgttgct ggcgctggca gaagaacgcg atattatcct
     5941 gctggatgaa tgggcggcgg atcaggatcc acacttccgt cgtgagtttt atcaggtgtt
     6001 gctgccgctg atgcaggaga tgggtaaaac tattttcgct atcagtcatg atgatcatta
     6061 ctttatccac gccgaccgcc tgctggaaat gcgcaatggg caacttagcg agctgacggg
     6121 cgaagagcgc gatgccgctt cgcgtgatgc cgttgcccgg acggcataat attacgacgc
     6181 tgacctggcg gactgctgcc gtcaggtcaa tatgctaaaa atcccttcat ttctaataac
     6241 cctataatta atcaacgaaa ttataatgtt tctaaaatta gaatataatt tataaacatt
     6301 atttaaatgt tgttacttaa gtgttaccgt tgatgccgcg caaatctcac ctgcaataaa
     6361 gcgactaaaa gtaaggcatt aacaagatga aaaaagtgac tgccatgctc ttctcgatgg
     6421 ccgtggggct taatgccgtt tcgatggcgg caaaagcgaa agcgtccgag gagcaggaaa
     6481 ctgatgtact gttgattggc ggcggcatta tgagcgccac gttggggacc tatttacgcg
     6541 agctggagcc tgaatggtcg atgaccatgg tggagcgcct ggaggggggc gcgcaggaga
     6601 gttcgaacgg ctggaacaac gccggaaccg ggcattctgc actgatggaa ctgaactaca
     6661 ccccgcaaaa cgccgatggc agcatcagta ttgaaaaagc agtcgccatt aacgaagcat
     6721 ttcagatttc ccgccagttc tgggcgcacc aggtcgagcg cggcgtgctg cgtactccgc
     6781 gttcatttat caataccgtt ccgcatatga gctttgtctg gggcgaggat aacgttaatt
     6841 tcctgcgcgc ccgttacgcc gcgttgcaac aaagctcgct gtttcgcggt atgcgttact
     6901 ctgaagatca cgcgcagatc aaagagtggg caccgttagt gatggaaggg cgcgatccgc
     6961 aacagaaagt agcggcaaca cgcacagaaa ttggcactga tgtgaactat ggtgagatca
     7021 cccgccagtt aattgcttcc ttacagaaga aatctaactt ctcgctgcaa ctcagcagcg
     7081 aagtccgcgc cctaaagcgt aatgacgata acacctggac cgttaccgtt gccgatctga
     7141 aaaacggcac tgcacagaac atccgcgcca aatttgtctt tatcggcgcg ggcggtgcgg
     7201 cgctgaagct gttacaggaa tcggggattc cggaagcgaa agactacgcc ggtttcccgg
     7261 tgggcggaca gttccttgtt tcggaaaatc cagacgtggt taatcgccat ctggcgaaag
     7321 tttacggtaa agcatccgtt ggcgcaccac cgatgtcggt tccgcatatc gatacccgtg
     7381 ttctggacgg taaacgcgta gtgctgtttg ggccatttgc caccttctca accaaattcc
     7441 tcaaaaacgg tgcattgtgg gatctgatga gttccaccac cacctctaac gtgatgccga
     7501 tgatgcacgt cgggctggat aatttcgatc tggtgaaata tctggtgagt caggtgatgt
     7561 tgagtgaaga ggatcgtttt gaagcgttga aagagtacta tccgcaagcg aaaaaagagg
     7621 actggcgttt gtggcaagcg gggcagcgtg tgcagattat caagcgtgat gccgagaaag
     7681 gtggcgtact gcgtctgggt accgaagtgg tcagtgacca gcaagggacc attgcggcgc
     7741 tgctgggggc atcgccaggg gcgtcaaccg ccgcgccgat tatgttgaat ctgctggaaa
     7801 aagtatttgg cgatcgcgtt tccagcccgc aatggcaggc tacgttgaaa gcgatcgttc
     7861 cgtcttatgg acgcaagctg aacggtgatg tagcggcaac agaacgcgag ttgcagtaca
     7921 ccagcgaagt gctggggctg aaatacgaca aaccgcaagc agcagatagt acgccgaaac
     7981 cgcagttgaa accgcaaccc gttcaaaaag aagtggcgga tattgcgttg taatgatgtg
     8041 ccacatccgg catggtatgc cggatgtggc gtatactgat aagacgcgcc agcgtcgcat
     8101 caggcaaccg gctcgggcgt tagatgttgg atgcggcgta accgccttat ccaaccaacg
     8161 gggtgccgta cttcacaaca gtttagcgaa cttccgcgtt gtcaattttc tcttccgcct
     8221 tccagacgcg gtatttcaca tctacattgt ctggcgtata caccacgatc ggcagcttgc
     8281 tgttgtaacg cagcattcca gcatcgccca gatacgcggt gacaaatttc ttctctttct
     8341 tgccatccgg gcaggccatc atcgtggaaa ccggcgagct aactttatca aagacataat
     8401 aatcatagcc ccagccttcc agcgttttgc tttccagctt cccgccgaga cgatgcaaat
     8461 tgcaatcgac ttccagcgtc tgaccgatta acagttctac tttcagggta gattcatctt
     8521 cttgcggggt taactgaatc acctgacgct tcatgcctgt ttcagcttgt ggataaggcg
     8581 cgattttttc cagtggctgg acgctttctg ccgcccaggc ggaagtggta gcgaaagcgg
     8641 caaacaatac tgcaggtaga atggtcttca tttttttgct ttccttcttc atttataggt
     8701 tttcaccgat gatgtcgctg aaggttaaca tattcatcag atttattaat ttgctttatg
     8761 aataacttaa agaacattca gacaatttta cctgctcttt tagtgttaaa ttctgatgag
     8821 ggagcaaacc agcagaaata tctctggtga atataatcgc tatataaata tgtttataac
     8881 catttgaaat gtgagcaaaa gcccgttttt gccacgcttc ccgccactct tttgatcctg
     8941 ctacaggttt taccccgatc ggggtatgca tctttgacac atcctttaat atcttagcgg
     9001 ctataaaaat ggcttattaa ttatgcggtc tatttggtcg ctctcaattt tcagagcgcg
     9061 ttaatgatgg aaggtcaatg tgaagattga tgcatcccgt cggggcatac tcactggtcg
     9121 ctggcgcaaa gccagtaacg gtatccgtcc gccctggtcc ggtgatgaat ctcattttct
     9181 gacccattgc acccgctgcg acgcctgtat taatgcttgt gaaaacaaca ttctgcaacg
     9241 cggcacgggt ggctatccga gcgttaattt caaaaataac gagtgcagct tctgttacgc
     9301 ctgcgcgcag gcttgccccg aatcgctatt ttctccgcgc cacaccaggg cctgggattt
     9361 gcagttcacc atcggggacg cgtgcctggc gtatcagtca gttgaatgcc gccgctgtca
     9421 ggatagctgt gagccaatgg caattatctt tcgcccgacg ctctccggga tctaccagcc
     9481 gcaacttaat agccaacttt gcaacggatg tggagcatgt gcagccagct gtccggtatc
     9541 agccataact gcggagtatc tccatgcaca ctaactggca agtttgcagc ctggtcgtgc
     9601 aggccaaaag cgaacgaatt tcagacatca gcacccaaca gaacgccttt cccggctgtg
     9661 aagttgctgt cagcgacgcg ccgagcggtc agttgattgt ggtggtggaa gcagaagaca
     9721 gcgaaacgct gatccaaacc attgagtcag tacgcaacgt agagggcgtg ctggcggtgt
     9781 cgctggttta tcaccagcag gaagagcaag gtgaggaaac accatgaaac tcagtcgtcg
     9841 tagctttatg aaagctaacg
//
LOCUS       DADLSA010000140         9796 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.141, whole genome shotgun sequence.
ACCESSION   DADLSA010000140 DADLSA010000000
VERSION     DADLSA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.141"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..335
                     /gene="tqsA"
                     /locus_tag="JN691_003326"
     CDS             <1..335
                     /gene="tqsA"
                     /locus_tag="JN691_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAY7244682.1"
                     /translation="YIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFGNI
                     LEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSIAV
                     LLSDLNKE"
     gene            complement(360..1748)
                     /gene="pntB"
                     /locus_tag="JN691_003327"
     CDS             complement(360..1748)
                     /gene="pntB"
                     /locus_tag="JN691_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAY7244683.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(1759..3291)
                     /gene="pntA"
                     /locus_tag="JN691_003328"
     CDS             complement(1759..3291)
                     /gene="pntA"
                     /locus_tag="JN691_003328"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAY7244684.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQSQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            3815..4759
                     /locus_tag="JN691_003329"
     CDS             3815..4759
                     /locus_tag="JN691_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7244685.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            4945..6327
                     /locus_tag="JN691_003330"
     CDS             4945..6327
                     /locus_tag="JN691_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7244686.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVSVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            6364..7086
                     /gene="folM"
                     /locus_tag="JN691_003331"
     CDS             6364..7086
                     /gene="folM"
                     /locus_tag="JN691_003331"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAY7244687.1"
                     /translation="MDKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTVPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(7083..7418)
                     /locus_tag="JN691_003332"
     CDS             complement(7083..7418)
                     /locus_tag="JN691_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAY7244688.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            7547..8290
                     /gene="rstA"
                     /locus_tag="JN691_003333"
     CDS             7547..8290
                     /gene="rstA"
                     /locus_tag="JN691_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAY7244689.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            8515..>9796
                     /locus_tag="JN691_003334"
     CDS             8515..>9796
                     /locus_tag="JN691_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HAY7244690.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGGGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSMMSGVISARLLKD"
BASE COUNT         2426 a         2476 c         2343 g         2551 t
ORIGIN      
        1 attacatccc taatattggt tcagttctcg cggcaatccc ccctatcgct caggtactgg
       61 tgtttaatgg cttctacgaa gcgttgctgg tgctggcggg atatctgctg attaatctgg
      121 tcttcggcaa tattctggag ccgcgcatca tgggacgtgg gctggggctt tcgacattgg
      181 tggtattttt gtcgttgatt ttttggggat ggttgttagg accggtgggt atgctgcttt
      241 ccgtgccgtt gacaattatt gtcaaaattg cgcttgaaca aacagcggga ggtcaaagca
      301 tcgccgttct gttaagcgat ctcaataaag agtgacggcc tccgcagagg ccgtcagggt
      361 tacagagctt tcaggattgc atccacgctg gctttggcgt caccaaacag catgtgggtg
      421 ttttccttga agaacagcgg gttttgcaca ccagcatagc cagtgttcat cgaacgttta
      481 aagacaatca cgttctgcgc tttccacact tccagcacag gcataccagc aatcggactc
      541 ttcggatcat cctgcgccgc cgggttaacc gtatcgttag caccaatcac cagtacggta
      601 tcggtatcag caaagtcatc gttgatctcg tccatttcca gcacgatgtc atacggtact
      661 tttgcttcag ccagcaatac gttcatatgt ccaggcaaac gccccgcaac cgggtggata
      721 ccgaaacgca cattaatacc acgagcgcgg agtttctcgg taatttcagc gacaggatat
      781 tgcgcctgcg cgactgccat gccgtacccc ggagtaatga tcactgaatg ggagtttttc
      841 agcagttccg ctgtctcttc tgcggtgatt tcgcggtgct cacccacttc ctgatcatcg
      901 ccagtagaag agccgtcggt gccgaaacca cccgcaataa cgctgataaa ggaacggttc
      961 atcgccttac acataatgta agaaaggata gcccccgaag aaccgaccag cgcaccggtc
     1021 acaatcagca ggtcgttgct gagcataaag cccgcagccg cagccgccca gccggagtac
     1081 gagttcagca tcgacaccac caccggcata tctgcaccac cgatggaggc gactaaatgc
     1141 cagccgaata ccagcgcaat ggcggtcatt atcagcaatg ccagcacttg caggccgacg
     1201 ctgtccgtgc gaacaaatac aatcagcagc aagaaggaaa cgaccagagc cgccaggttc
     1261 attttgtgac ggtttggcag catcaacggt ttagacgaaa tcttgccaca cagtttgccg
     1321 aacgccacca ccgaacccgt gaacgttacc gccccgatga agataccgag gaacacttcc
     1381 gtcaggtgaa tattgaccag aatcggtgcc attcccgcgt catgatgcag ataactgtta
     1441 aagccaacca gcactgccgc cagacccacg aagctgtgca ggatcgccac cagttctggc
     1501 atttcggtca tttcaacttt cttcgccagc cggataccaa ttgccccacc aatgaccatc
     1561 gccagcaaga tccagccaac attacccgta tccggtccaa aaatggttgc gattaacgca
     1621 atcgccatcc cggcgatacc gaagttgtta ccctggcgag acgtttcatg tttcgaaaga
     1681 ccggccagac tgaagataaa caggatcgcg gcaacaatgt atgcagctgt aactaatcct
     1741 ccagacatat gttacccctt aatttttgcg gaacattttc agcatgcgct gagtcacggt
     1801 gaagccaccg aaaatattaa tgctggctat aagcaccgcg ataaaactaa ggaagctaac
     1861 ccagccgccc tggccaatct gcaacagtgc tccgacaaca ataatccctg aaatcgcgtt
     1921 ggtaaccgac atcaacggtg tatgcagcgc gtgcgataca ttccacacca cgtaataacc
     1981 gacaacgcag gccagcgcga aaacggtgaa gtgcccaaga aactctttcg gcgcaacgct
     2041 tgacagccaa ccgaacagga tgattgccag cgccatcaac gcgtatttac gccacggtga
     2101 gcaggcacat ttttcctcag ttttcacttc cggtgccgct ttttgtgccg cctgcgactg
     2161 agctgatacc tgaatcggcg gtgccggcca ggtaatttcg cccgcacgga tcacggtcac
     2221 gccgcgaatc accacatcat caaaatcaac agtgatattg ccgtctttct ctttgcacaa
     2281 cagtttcagc agattaacga ggttagtacc gtaaagctgt gaggattgcg tcggcagacg
     2341 gcccggaaga tcggtataac caatcacttt gacaccattt tccgtactga agatttcacc
     2401 cggcacggtg tattcacagt tgccaccgtt ttgggctgcc aggtcgacaa tcacactgcc
     2461 cgccttcatg gagtcaacca tttcacgggt aattagcttc ggcgctggtt tgcctggaat
     2521 aagcgcggtg gtgacaatga tatcgacctc ttttgcctgg gcggcaaaga gttccatttc
     2581 cgctttgatg aacgcgtccg acatcacttt ggcatagcca tcgccgctgc ccgcttcctc
     2641 tttaaaatcc agctcgagga attccgcgcc catactttga acttgttctt tcacttccgg
     2701 gcgggtgtcg aatgcacgca caatcgcgcc gagactgttt gctgcgccaa tggcggccag
     2761 acctgcaaca ccggcaccaa tcaccatcac ttttgccggt ggcactttcc cggccgcagt
     2821 gatttgcccg gtaaagaagc gcccaaattc atgtgccgct tcaacaatgg cgcgataacc
     2881 ggcgatattc gccatcgagc ttagtgcgtc cagcgattgt gcgcgtgaga tacgcggcac
     2941 agagtccatc gccatcacgg tcacgttacg ttccgcaagt ttttgcatta attccggatt
     3001 ctgcgcaggc cagataaaac tcaccagcgt tgtcccggga ttcagtaacg caatttcatc
     3061 atctaacggc gcattgacct tcagaatgat ctctgactgc cagacgctat tcccttctac
     3121 aatttcagcg cccgcttgca caaacgcttt atcgtcaaaa cttgccagtt gacccgcgcc
     3181 gctctctacc gcgacggtaa aacccagttt cagcagctgt tccactgttt ttggcgttgc
     3241 tgcaacacgg gtttcattgg ttaaccgttc tcttggtatg ccaattcgca tgatattccc
     3301 ttccatcggt tttattgatg atggtttgcc tgtgtcagga gccacacaag ccgctcatgt
     3361 acgagctaaa tgttactccg ttaaaataaa ttagtaacaa acgccctata acgtactgaa
     3421 aattatgcct gtgatctagc gccaaaaatc agtatttcgg cgtgaactcg caaaatatta
     3481 acgattcagc cgtgatagtg ggataaacac cttagaacgc cggataaaga ctgataattg
     3541 tcttcgacgg tcgggtaaaa cgagacatcg ccccggcacg aatcactact taacattaaa
     3601 ttaacttata caattcagtt gcttcagtag taatgatgct gatacggctg ttttttaagc
     3661 atagacggtc atttgagcag gattaaaatt ggtttaagga atgtgatatg aaaaatgacg
     3721 cagacagtta caccgtttaa atgcaataat cagccacgtt tctcgttaat aacaatacca
     3781 gtacctggtt tgcgcaaggc gaaggattat ttttatgaag cttaagaaca ccctcctggc
     3841 gtcggcactg ctttctgcta cggcattctc cgttaacgca gcaacagaac tgacaccgga
     3901 gcaagcggca gcggttaaac cttttgaccg tgtagtggtt accggtcgtt ttaatgctat
     3961 tggcgaagcg gtgaaagccg tttctcgtcg cgcagataaa gaaggtgccg cctcttttta
     4021 tgttgtcgac acttctgatt ttggtaacag tggtaactgg cgtgtggtcg ctgacctcta
     4081 taaagccgat gctgaaaaag cagaagaaac aagtaatcgc gtaattaacg gtgttgtcga
     4141 actgccgaaa gatcaggctg ttctgattga accgtttgac acggtcaccg tccagggctt
     4201 ctatcgtagc cagccagaag tcaatgatgc catcaccaaa gcggcaaaag cgaaaggtgc
     4261 ctactctttc tacatcgttc gtcaaatcga tgccaaccag ggcggcaacc agcgtattac
     4321 tgcattcatc tataaaaaag atgctaagaa acgtatcgtc cagagcccgg atgtgatccc
     4381 ggcagattcc gaagcaggac gtgcagctct ggctgccggt ggcgaagccg cgaagaaagt
     4441 tgagatcccg ggtgttgcga ctaccgcatc accaagttct gaagtcggtc gcttctttga
     4501 aacccagtca tcaaaaggcg ggcgttacac cgtcacgctc ccggatggca ctaaagtcga
     4561 agaactgaac aaagcgacag cagcgatgat ggtcccgttc gacagcatca aattctctgg
     4621 caactacggc aacatgaccg aagtctccta tcaggttgcg aaacgtgccg cgaagaaagg
     4681 tgctaagtat taccacatca cccgccagtg gcagaaacgt ggtaataacc tgaccgtcag
     4741 cgcagatctg tataaataac tattcagtta ctcctataag gcggcttgat gccgcctttc
     4801 gcatttttag tcacaaattt ccccgcgacc attgcatgca gacacatctc ttcgtaaaat
     4861 cccgcgcttc agtggcaaac tccattttta tgcgttttgc cctaatattt attcttctta
     4921 tcacgtttta atcactggat atcgatggaa aagaaactgg gactgagcgc actcaccgcg
     4981 ctggtattaa gctcaatgct gggcgcgggt gttttcagtc tgccgcaaaa tatggcggca
     5041 gttgccagcc cggcagcact gctcatcggc tggggtatta ctggcgctgg cattttattg
     5101 ctggcctttg ccatgctgat cctcacgcgc attcgtcccg aacttgatgg cggtatcttt
     5161 acctatgccc gcgaagggtt tggcgagcta atcggctttt gttccgcatg gggatactgg
     5221 ctgtgcgcgg tcatcgccaa cgtctcctat ctggtgatcg ttttttccgc gttaagcttt
     5281 tttacggaca cgccggaatt gcgcctgttt ggcgatggta atacctggca atcgatagtc
     5341 ggtgcatcgg ctttattgtg gattgttcac tttttgatcc tgcgcggggt gcaaaccgct
     5401 gccagcatta acctggtggc gacactggca aaattgttgc cgctgggtct gtttgttgtg
     5461 ctggcgatga tgatgttcaa actggatacc ttcaagctcg acttcaccgg acttgcactt
     5521 ggcgtatctg tttgggaaca ggtgaaaaac accatgctga tcaccctgtg ggttttcatt
     5581 ggtgtggaag gtgccgtggt tgtttctgcg cgtgcgcgta ataaacgtga tgttggcaaa
     5641 gcgacactgc tggcggttct ctccgctctg ggcgtttact tgctggtaac gctgctttcg
     5701 ctgggtgtgg tggcacgtcc tgaactggct gaaattcgta acccgtcaat ggccggtctg
     5761 atggtggaaa tgatgggacc atggggcgaa atcatcatcg ctgccggttt gatcgtttcc
     5821 gtttgcggcg cgtacttaag ctggaccatc atggcggcag aagttccgtt cctggctgcc
     5881 actcataaag cattcccacg catttttgcg cgccagaacg cacaagctgc gccatccgcc
     5941 tcactgtggt tgaccaacat ctgcgtgcaa atttgtctgg tactcatctg gctgaccggt
     6001 tccgattaca acacgttgct gaccatcgcc tcagaaatga ttctggtgcc ctatttcctg
     6061 gtcggcgcgt tcttgctgaa aatcgcaacc cgtccactgc ataaagcggt aggtgtcggt
     6121 gcctgcattt atggcttatg gttattgtac gcctctggtc caatgcactt gctgttatcc
     6181 gttgttctgt atgcaccggg gctgctggtt ttcctgtatg cgcgcaaaac gcatacacat
     6241 gataatgtac tgaaccgtca ggaaatggtg ctgattggca tgctgttaat tgcctcagtt
     6301 ccggcgacct ggatgctggt gggataacgt tcatcccatc gttggatagc aaaggagata
     6361 acgatggata aaacccagcc cttgccaata ttaattactg gcggaggtcg tcgcatcggc
     6421 ctcgccctcg catggcattt cattaatcaa aagcaaccgg tgattgtcag ctatcggaca
     6481 cactatccag ccattgatgg actgattaat gcaggtgcgc agtgtattca ggctgatttt
     6541 tcgaccaacg acggtgtgat ggcgtttgcc gatgaagtac taaaaaccac ccatggtctg
     6601 cgtgctattt tgcataacgc cagtgcgtgg attgcggaaa aaccgggtgc gccactggcc
     6661 gacgtactgg cttgcatgat gcagatccac gttaataccc catacctgct caaccatgcg
     6721 ctggaaagat tactgcgtgg gcacggacac gccgccagcg atatcattca ctttaccgat
     6781 tatgtggtgg agcgcggtag cgacaaacat gttgcgtatg ctgcaagcaa agcggcactg
     6841 gataatatga cccgctcgtt tgcccgcaag ctggcaccgg aagtgaaagt gaattctatt
     6901 gcgccatcgc tgatcctgtt taatgaacat gatgatgccg aatatcgaca acaggcgctg
     6961 aataaatcac tgatgaaaac cgtgcctggc gagaaagaag tgatcgacct ggtcgattac
     7021 ttacttacca gttgctttgt caccggacgc agtttcccac ttgatggcgg tcgtcatctg
     7081 cgttaatgca gctttatcca gcatataatc aacacccatg cgcttatccc ccaacacgca
     7141 acagaaccaa caaaagcggc gggcaagcgc atcattccgg tgaaatacca cagcgacacc
     7201 aggtaaacaa aatagggaat aatcgaccac atactaaaaa tgatggttgc gcgtaaggct
     7261 tcaatgccgc gttcgctggc aacaatataa tgcgcgataa gcgcaaaggt cgggaaaagt
     7321 ggaatcagcc cggcgatata ataatttttc gtttttgcta aaacaccaat caacagcact
     7381 accagcgcac cgagcgcggc tttgattacc agccccatct ttttacctta acacttccat
     7441 aacaagtcat cagtagaata cctgatgaaa acttgtttag aaacgattga tagtaagtaa
     7501 aaacagcgcg gtgtattgtg acgtttttat atctaccgtg aatgttatga acactatcgt
     7561 atttgtggaa gatgatgcgg aagtcggttc actgattgcc gcgtacctgg caaaacatga
     7621 tatgcaggtt accgtagaac cgcgcggcga ccaggccgaa gaaaccattt tgcgagaaaa
     7681 tccggatttg gtgttactcg acatcatgct accaggcaag gacggcatga ccatttgtcg
     7741 tgatttacgc gcaaagtggt ctggaccgat tgttcttcta acctctctcg atagcgatat
     7801 gaaccacatc ctggcactgg aaatgggtgc ctgcgactat attctcaaaa cgacgccccc
     7861 tgctgttttg ctagcgcgtt tacgtttgca tttgcgtcag aatgagcaag ccacactgac
     7921 caaaggtctt caggaaacgt ctctgactcc ctacaaagcc ctgcatttcg gcacgttaac
     7981 tatcgacccc atcaaccgcg tagtcacctt ggctaacact gaaatctcgc tctcgacagc
     8041 tgatttcgaa ttattgtggg aactagctac gcatgccggg caaatcatgg accgcgatgc
     8101 attgctgaaa aatttacgcg gcgtcagtta tgacggactg gatcgtagcg tggacgtggc
     8161 tatttcgcgg ttaagaaaaa aactgctcga taacgccgca gaaccttatc gcattaaaac
     8221 tgtgcgtaac aaaggctatc tttttgcgcc tcatgcatgg gatagtgaca ataaacaatc
     8281 agatgactaa tatttgttca tctgaaaaag agaacaatta acgaattcaa ttaataagag
     8341 cttattcaaa ttccaaaccc cctacatttg atatgaatat gagaaaaaat ttctctataa
     8401 tcgcctcgtc cctgcgtctt atggcgcaga ttttttacaa caaactcttt ccggcatttc
     8461 aggtgctgaa tgaggcaaga ggcttaaaaa acggttacaa ctttcacata tattgtgctt
     8521 tttttgatct gctcatgggg gattttcaaa acggatcaaa agagcgaatc cctttccaaa
     8581 agtgttttta tcgcgatttt cctgggggct atcattggta tctccctgca tttcattcct
     8641 gagacaagca gtgaaaccgt tatcgaatgg tataatattg ctggtaacgg ttatgttaat
     8701 ttattaaaac ttgttgccgt tccactcata ttcatttcga ttctttcagc gattaataag
     8761 ctcgaaaata gcgctggtat tggcaagatg tctttaacta ttgttgcctg tatgttgtgt
     8821 ttagtcatga ttgcaggttt agttggtctg gctattgcca gtgcaattgg ccttgatgcc
     8881 agcgcctttg gtcatatgca atcctcttta accagcgatg acgtctcgaa aacagctgcg
     8941 gtatctttac cgcgactcat tacgtcattg atcccaacca acctcttcct tgatctgacc
     9001 ggggcaagaa gtgtttcggt tattggcatc gtcatcttta caattcttgc tggtattgcg
     9061 ctgttaaaag taaagaaaga tgcaccagca gagggcgaaa aattgagcgc ggggatcaac
     9121 gcgattcaaa tatgggtaat gaaaatggtc cgtattgtca ttgcgctgac gccttatggg
     9181 gtaatggcac taatggcgaa agtcttctca acgtatcagc tttcacagtt taccaatttg
     9241 ctcgggttca tcgtcgcctg ttatctggcc gtttttgtga tgtttattgt tcactcgggc
     9301 attttgctgt taataggtaa aaaccccgtt aactattaca agaccgtctg gcaagtactt
     9361 acctttgcgt ttgtttctcg cagtagtgcg gcttcaattc ctctggctat ttctgcattg
     9421 gaaaaattgg gggttccaaa tacgattgct aatattgcag cgtcttttgg ttccagcatg
     9481 ggacaaaatg gctgcgctgg gatttatcct gccgtgatgg tggcgatgat tgctccaaca
     9541 atgggtatcg atcccttctc attgcaattc ctcgcaactc ttttacctgc catcgcgctt
     9601 ggctcaatag gggtagcagg cgtaggtggt ggtgggacat tcgcggcgct gattgtcctt
     9661 tcaacactta atttccctgt cgcattagta gttattttta ttgccataga gccgatcgtt
     9721 gatatggcaa gaacagcgct taatgtgaat ggttcaatga tgtccggtgt aatatcagcc
     9781 cgtcttctta aggata
//
LOCUS       DADLSA010000141         9734 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.142, whole genome shotgun sequence.
ACCESSION   DADLSA010000141 DADLSA010000000
VERSION     DADLSA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.142"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="JN691_003335"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="JN691_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAY7244691.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="JN691_003336"
     CDS             1828..3147
                     /locus_tag="JN691_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAY7244692.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3524..4669
                     /locus_tag="JN691_003337"
     CDS             3524..4669
                     /locus_tag="JN691_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7244693.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFYSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDCDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKGSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="JN691_003338"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="JN691_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5873
                     /gene="ddpX"
                     /locus_tag="JN691_003339"
     CDS             5469..5873
                     /gene="ddpX"
                     /locus_tag="JN691_003339"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HAY7244694.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACVDNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRRPQQAQAMLWQACPDPQYVVDVTVGSNHSRG
                     TALDLTLRDDVEVVNDAKRGNVRRTPTKTNAI"
     gene            5818..6714
                     /locus_tag="JN691_003340"
     CDS             5818..6714
                     /locus_tag="JN691_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HAY7244695.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6711..7697
                     /locus_tag="JN691_003341"
     CDS             6711..7697
                     /locus_tag="JN691_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7244696.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7690..8616
                     /locus_tag="JN691_003342"
     CDS             7690..8616
                     /locus_tag="JN691_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY7244697.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKSTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTASAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHECEVRQSLAIREDGRELRCWRAL"
     gene            complement(8672..9103)
                     /gene="osmC"
                     /locus_tag="JN691_003343"
     CDS             complement(8672..9103)
                     /gene="osmC"
                     /locus_tag="JN691_003343"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAY7244698.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            9449..9664
                     /gene="bdm"
                     /locus_tag="JN691_003344"
     CDS             9449..9664
                     /gene="bdm"
                     /locus_tag="JN691_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HAY7244699.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
BASE COUNT         2414 a         2364 c         2419 g         2537 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cttggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccacca
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctattc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggattgcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagg ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg ccgcccacaa caagcacagg
     5701 cgatgttgtg gcaagcctgc ccagacccgc aatatgttgt tgatgtgacg gtcggttcta
     5761 atcacagccg tggcacggcg ctcgacctga cgcttcgtga tgacgtggag gtggtgaatg
     5821 atgctaagcg aggaaacgtc cgccgtacgc ccacaaaaac aaacgcgatt taacggtgca
     5881 aaactggtat ggatgctgaa aggcagtccg ctcaccgtga ccggcgcagt catcattgta
     5941 ttaatgctac tgatgatgat tttttcaccg tggctggcga cgcatgatcc caacgccatt
     6001 gatttaaccg cccgcctttt gccgccttct gcggcgcact ggtttggcac cgatgaagtg
     6061 ggacgcgatc tgtttagccg cgtactggtc ggcagtcagc aatcaattct cgccggatta
     6121 gtggtggtcg ccattgcggg tatgattggt tcgctactcg gatgtctatc cggtgtgctt
     6181 ggcggacgcg cagacgccat catcatgcgc atcatggaca ttatgctgtc gattccttcg
     6241 ttggtactga caatggcact ggctgccgct ctcgggccga gtttatttaa cgccatgctg
     6301 gcgattgcta ttgtgcgaat tcccttttat gtgcgcctgg cgcgggggca agcattagtt
     6361 gtacgccagt atacctatgt tcaggcggcg aaaacctttg gcgcgtctcg ttggcatctg
     6421 atcaactggc atattttacg taactcccta ccgccgctga tcgtgcaggc atcgctggat
     6481 atcggtagcg cgattttaat ggccgccacg ttgggattta ttggcctggg tgctcaacaa
     6541 ccgagtgctg aatggggcgc gatggtagcg aatggtcgca actatgtact cgatcagtgg
     6601 tggtattgcg catttccggg ggcagcgatt ttgcttaccg ccgtcgggtt taatctcttt
     6661 ggtgatggta ttcgcgatct gcttgacccg aaagcaggag gaaagcagtc atgacccaac
     6721 ccgttctgga cattcaacag ctgcatttga gtttccccgg ttttaacggt gatgttcacg
     6781 cgctcaacaa tgtgtccttg cagattaacc gcggcgaaat tgtcggtctg gtgggagaat
     6841 ccggctcagg taaatcagtc accgcaatgc tgattatgcg tctgctaccg acgggcagtt
     6901 attgcgtaca tcgggggcag atttcactgc tgggagaaga tgttcttaat gctcgggaaa
     6961 aacagcttcg tcagtggcgc ggcgcacgag tggcgatgat ctttcaggaa ccgatgaccg
     7021 cccttaatcc gacacgtcga ataggtcttc agatgatgga cgtaatccgc catcatcaac
     7081 caataagtcg tcgggaagcc agagctaaag cgattgccct gctggaagag atgcaaatcc
     7141 cggatgccgt ggaagttatg tcgcgctatc cgtttgagct ttcaggtggt atgcgccagc
     7201 gggtaatgat tgcgctggca ttctcctgcg agccgcaatt gattattgcc gacgaaccga
     7261 cgacggcgct ggacgtcaca gtacagttgc aggtactgcg tttgcttaaa cataaagcac
     7321 gcgccagtgg aactgcggta ctgttcatca gccatgatat ggccgtggtg tcgcaactgt
     7381 gcgatagcgt ttacgtgatg tatgccggaa gcgtaattga aagcggcggg acggcagacg
     7441 ttatccatca tccccggcat ccgtatacca ttggtttgct gcaatgcgca ccagaacatg
     7501 gagtaccacg ccagccatta cccgccattc cagggacggt accaaacctc acccatttgc
     7561 ctgacggctg cgcttttcgc gatcgttgct atgcggcagg tgcacagtgt gaaaacgtcc
     7621 cggcgctgac agcgtgtggt gacaacaacc agcactgcgc ctgttggtat ccgcagcagg
     7681 aggtcataag tgtctgacac gttattaacg ttacgcgacg tccatatcaa ttttccggcc
     7741 cgtaaaaact ggcttggtaa atctacggaa catgttcatg ccattaatgg tattgattta
     7801 cagatccgcc gtggtgaaac cttagggatc gtcggcgagt caggctgcgg caaaagcacc
     7861 ctcgcacagc ttttaatggg catgctgcaa ccgagccacg ggcagtacat ccgttcaggc
     7921 tcacaacgca ttatgcagat ggtgtttcag gacccgctct cttcgcttaa tccgcgctta
     7981 ccggtgtggc gcatcatcac agaaccgctc tggatagcta agcgtagtag tgaacaacag
     8041 cggcgagcgt tggcagagga gttggctgtg caggtgggta ttcgtccgga gtatctcgac
     8101 cgcctgcctc atgcgttctc cggcgggcag cggcaacgca tcgccattgc cagagcactc
     8161 tcttcgcagc ctgacgtgat tgtgcttgat gagccaacct cggcgctgga tatctccgtg
     8221 caggcgcaga tcctcaattt actggtaacg ctacaggaaa atcacgggct gacttatgtg
     8281 ctgatttcac acaatgtctc ggtgatacgt catatgagcg atcgggtggc ggtgatgtat
     8341 ctcgggcaga ttgtagaact gggcgacgcg caacaggtgt tgactgcttc ggcacatcca
     8401 tacacccgat tattgctgga ttccctcccc gccattgata aaccgctgga ggaagaatgg
     8461 gcattacgta aaacggatct gccgggaaac cgcacgttgc cgcaaggctg ttttttctac
     8521 gaacgttgcc ctctggcaac ccacgaatgt gaagtccggc aatcattagc aataagggag
     8581 gacggacgtg agctccggtg ctggcgggcg ctgtagagat gctaacaacg catcaggcat
     8641 tcacgcgcct gacgcgtcat ccggcaatgc tttacgattt caactggtaa tccagcgtaa
     8701 tttccgcttt cagtacctga gagaccgggc atcctgcttt tgctttctgg atgatgccgt
     8761 caaaggtaga ggcatcaata cccggcaccg caacttcact cttcagtgcg attttcgtaa
     8821 tcgcaaaacc ggcatccact ttatccagcg acacatcggc ggtggtatca attgatgttg
     8881 gcgtgaatcc cgcttccccc agcattaatg aaagcgccat tgagaaacat gcggcatgcg
     8941 ctgcgccaat cagttcttca gggttggttc ctttttcgcc ttcaaaacgc gtgttaaatc
     9001 catacggctg ttggttcagc acgccgctct cggtggatac tgttcccttc ccgcgtttga
     9061 tatcgccttc ccagtgtgcc tgacctttct tatggattgt cattgttgct ctcctgtggg
     9121 cttaaaaagg aaattataga ctacgtgaga aacagcacga ggataagcag gatattccga
     9181 ataaaatacg gcagaaaata ttacttctgc acttaaatta aattcccaca atagataacc
     9241 gggaatatat atctcaagag atattgcatc aatatcaatc aactcaccac aataataata
     9301 cctatcaata tcatggggtt aaaatataaa aatcatcaac taggactaat ctcaacaacg
     9361 aagtggtaaa tatttgccat gctgaatgtg ctttataaat ctgcgatccg tagcagacac
     9421 cataaataca cagacacgga gaatcactat gtttacttat tatcaggcag aaaattcaac
     9481 agcagaaccc gctctggtta atgccattga gcaaggcctt cgggcagagc acggtgttgt
     9541 cactgaagat gacattctca tggagctgac caaatgggtg gaagcttctg ataacgacat
     9601 cctcagtgat atctaccagc aaacgatcaa ttatgtggtc agtggccagc accctacgct
     9661 ttaaggtgct atgcttgatc ggcaacctaa tttaggggtt tagcacgtgt ttcttcgcta
     9721 cggcgatgtt gtcc
//
LOCUS       DADLSA010000142         9593 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.143, whole genome shotgun sequence.
ACCESSION   DADLSA010000142 DADLSA010000000
VERSION     DADLSA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.143"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..928)
                     /locus_tag="JN691_003345"
     CDS             complement(<1..928)
                     /locus_tag="JN691_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HAY7244700.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDADIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYQNDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKVPQSEAGITRLLSSVEKSLDQAV"
     gene            complement(932..1495)
                     /locus_tag="JN691_003346"
     CDS             complement(932..1495)
                     /locus_tag="JN691_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244701.1"
                     /translation="MGEFRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETSWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGTAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLTPFPELINQQWVRRYDM
                     TVRLRRKVVREYGIKSLVEAPVIFFGD"
     gene            complement(1470..1859)
                     /locus_tag="JN691_003347"
     CDS             complement(1470..1859)
                     /locus_tag="JN691_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HAY7244702.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELLPFDGGIPVEQ"
     gene            complement(1846..2400)
                     /locus_tag="JN691_003348"
     CDS             complement(1846..2400)
                     /locus_tag="JN691_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244703.1"
                     /translation="MSFKSGVTTRVDNAKAILDALRSLTKKDVLVGIPSEDSERDDVP
                     FGNAGIGYLNEYGSPEQNIPPRPHLVPGVKSAEEQTVPQLKAAAQAALDGNAAGAESA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRASGESPGTELA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            complement(2397..2804)
                     /locus_tag="JN691_003349"
     CDS             complement(2397..2804)
                     /locus_tag="JN691_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HAY7244704.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLTE
                     NVTGKKLFPYFAGLFVAHYMTLWAADSRAMLAGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            2803..3036
                     /locus_tag="JN691_003350"
     CDS             2803..3036
                     /locus_tag="JN691_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244705.1"
                     /translation="MFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPRTV
                     GKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(3033..3974)
                     /locus_tag="JN691_003351"
     CDS             complement(3033..3974)
                     /locus_tag="JN691_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HAY7244706.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DISIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNQLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYGDGI"
     gene            complement(3986..4135)
                     /locus_tag="JN691_003352"
     CDS             complement(3986..4135)
                     /locus_tag="JN691_003352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244707.1"
                     /translation="MSSSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADGNAE
                     ISWKI"
     gene            complement(4119..4493)
                     /locus_tag="JN691_003353"
     CDS             complement(4119..4493)
                     /locus_tag="JN691_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244708.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRSYVTVN
                     LGSDFDASTIKKGDPVYVVVIH"
     gene            complement(4497..5717)
                     /locus_tag="JN691_003354"
     CDS             complement(4497..5717)
                     /locus_tag="JN691_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HAY7244709.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(5721..6464)
                     /locus_tag="JN691_003355"
     CDS             complement(5721..6464)
                     /locus_tag="JN691_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAY7244710.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQP
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(6355..7821)
                     /locus_tag="JN691_003356"
                     /pseudo
     CDS             complement(6355..7821)
                     /locus_tag="JN691_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            complement(7821..9224)
                     /locus_tag="JN691_003357"
     CDS             complement(7821..9224)
                     /locus_tag="JN691_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAY7244711.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINHDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            complement(9175..>9593)
                     /locus_tag="JN691_003358"
     CDS             complement(9175..>9593)
                     /locus_tag="JN691_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244712.1"
                     /translation="RRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
BASE COUNT         2168 a         2713 c         2334 g         2378 t
ORIGIN      
        1 gaacggcctg atccagtgat ttttcaacac ttgataacag tcgggtaata ccggcttcac
       61 tctggggtac tttcgtggtg ctggtataaa gcaggttata gaggttggtc tgcacataat
      121 tctgtaacca gtccaggccg tggcgttcat caaagaaatc gccgttagcc atcactccct
      181 gctggaggat agccgtatca ttctggtagt acacgaatac attgcagttt tttgcatcaa
      241 gtgccgatgc ctggctgact gtcagtgttt catacccgac acccggctcc tgcttaaact
      301 tgagcgtaat cgcggtatta ctgccattga aattaaccgt gaatgcccgg ccaaatgcag
      361 ataacgcagc gtatttatta cccgatgaat actgaataaa actgcgtgaa tatccggcgg
      421 ttttcagttt tgatgccaaa tcatcgctgg atgcagtctg caggcatttc tcatcgcttg
      481 tcgtaatcgc cagaatacgg cttacagaag aggattcgat cgccgcagcc actttcagcc
      541 agtctgcatc cggaatatct gcatcgtctg caatccccag cccataccat gaagtataat
      601 caagcatggc attcacagcc tgctccagcg tctcaggcgt ggcctgttcg ctgtctccct
      661 tcgttttcac ccaacgacca acaaaaacct cctgaggttt cggtgattgt gagaaaaaca
      721 cctgcgcagc cttatattct ggtgattcca cgccaaaatc ttttccaata tcttccgcag
      781 cagaataacg gcgaatgcgc tcacttaccg gaatgattgt ggacgggccg agaatgagta
      841 atgcaccaaa atttcgccct gatgctgcac gcggcgacat gatcacatca acattaacaa
      901 cgtttgatac aggcaagccc tgtgccatag cttaatctcc gaaaaagatg actggtgctt
      961 ccaccagcga tttaataccg tactcgcgca caaccttccg gcgcagacgc accgtcatat
     1021 cgtagcggcg gacccattgc tgattaataa gttcagggaa gggagtcaga cctgtgtaat
     1081 cgccaagaga cagccccagc gcattcagtg ctgcattgtt ctgcggtaca gatataccgt
     1141 cacgaaaccg ggacgcatac accatccccg ccgtaccata aaacgaagcc atacactcaa
     1201 tcgtttcatg ccgccagagc tgagagccat catcggtctg tctggtgaat gccggactgt
     1261 catcacctga ccatccgata accccaaacg cacaccagct cgtttcaacc ggtagcagtg
     1321 gcggctgctc tttctgccag cgcggacgaa ccatcccggc agacagaccg gaaacgttac
     1381 gcatccactg gcttaacagc ctgtcgagcg cttcgtcata atccggatcg ccactggttg
     1441 gtatcagcca tccgcgctct gtgctggtgt tattgctcaa ccggaattcc cccatcaaac
     1501 ggcagcaact cacaatgcgc ctgaacgaat ccggcaccat acgctgtata cgggtcgacg
     1561 aaagtcacac gataatcacg gccctgatac gtcacgatat cggcatcacg gccagtctgt
     1621 ccctgcgtca gtcgctcagt cgtcacaatc agaattgcac cactgattac ctgcccggcc
     1681 tgcatacgac ggttttccag agagcgatca acagtaacga ctccggcaaa ctgcttttta
     1741 acttcgctgt cgctgccgat cccatcctca tccaccgttt gcacacggcg tgttacccac
     1801 aaattgaagt cgcaaaaatc ggggtcaaaa agcacatctg ttacatcaag agtcggcatc
     1861 tttatccctc acaacatggg taatcgctct gcgatattgc ccggtgtcaa ttaatggttt
     1921 cgccagttcg gttcccggag attcgccaga agcacgccgg gcaagttcca gtgttgcccc
     1981 cttgcgcccc cgacgagccc gggcttcaac agtactgtca gcaagcggcg taaagccggt
     2041 aatggtcatg taacgcctga cgccattagc ggccagtgtt ccggcacggt tgagtgcgct
     2101 ttctgctccc gcagcattac catcaagtgc agcctgcgcc gcggctttga gctgcggcac
     2161 cgtctgctct tctgccgatt taacgccggg gaccaagtga ggtcgtggcg ggatgttctg
     2221 ctctggtgag ccgtattcgt tgaggtaacc gatgcccgca ttaccaaacg gaacatcatc
     2281 ccgctcgctg tcttccgaag ggatgccgac cagcacatct tttttggtta acgacctgag
     2341 cgcatccaga atggccttag cgttatccac cctcgttgtt acaccgcttt tgaaactcat
     2401 agctggcgac cgcctgcacc gaacatcgtg atcaactgat aaaattcagc gccatatcgg
     2461 gtgttattcc agaaacctgc atcaggattc agcgtcgcgc tggtgtcata gctgacgctt
     2521 accttgtcaa cggactttga ggactgaaca ccattggttg aaccgcccgg accaccagcc
     2581 agcatcgctc tgctgtctgc cgcccagagc gtcatgtagt gcgcaacgaa caatccggca
     2641 aagtacggaa acaacttttt gccggtgacg ttttcggtca gcagttcatc ggccagattc
     2701 agacgaaact ggatttgcgc ttcgggatat ttggcagggt cagcaaactg cgggaagtcg
     2761 cggcgaaaat cacttaccgc tggcagactt tgattctttg gcatttttta cctcgttacg
     2821 cgcgtctgtg gctttgccaa cggatacttc cgcgtgcgca cgagtgaacc agtgcgtggc
     2881 aacgtcttcc tccacaacat gacggccttt aacaaactcg cgccgagaac cgtcgggaag
     2941 cgtgagcaca aacggggtat gtacgtgtat tactgcatca ttttttgcca tcgggtcatc
     3001 cttaatggcc ccgccagggg gccatgtggc tgttaaatgc catcaccgta cgaaatggtt
     3061 tctttgtaca ctggctcgac tgcacccagc ttgccgtagt aagtgacgat ctgatacaga
     3121 ccgcgatact gcaccggcac gctctgaagc ggaaccagcg ggtagcggac gtatttttta
     3181 tcgttggtgt acgcaaccat gcgatcctta ttccccacac cacggccttt cagccattta
     3241 accgcgcgga tattcagcgg aacaccgttc tggtgatagc tgatggtgtt ggtctgaagg
     3301 tacgtcaaca gggactggtt acctgcagat gaaacgatga tgctggacaa cagagcaaac
     3361 tgctcaggcg ggatcagcaa atcacgcgga accacagagt aaccagaagc ggcccacgca
     3421 tcagacagca cctggttaat gcttgcgcgg atttcgtccg gtgttgaggt tgcccacgtt
     3481 ttggcagcgt tgttgacagg cacgccgtcc agggtaacaa ggcctttcag gtttaatgcg
     3541 gaatcgccaa catatacctg ttcatcgtta tccatctgcc atttcagttg cataccgtca
     3601 tacttctgcg tatcaatcgg gcggccgacc tgctgagcag cctgcaattc tatgaccgtc
     3661 cagccaagtt ccatccccca caggttcagc tggttaccgg atttgccgat atccacgttc
     3721 acgccagcaa tagcggttga gtctttgcct acccagtttt ttccattcgg atttgcgcca
     3781 gtacccgcag cggcgaagct ggtattcgtc cagctggaaa tgtcatctgc gatggagatg
     3841 tcttcacgca gttgaatatc gcggctccag gtgtacccca ccagtggcag gttcagcgtc
     3901 tggtcgagtc gctccagctc cccgatgaga aaggcaccag agctgtcaac ggttgcctga
     3961 tcaaaagtaa tcattcgtct gttccttaaa tcttccagga aatttctgca ttgccgtcag
     4021 catcaccggc acctgtgaat tcagcgttgg tcagcaccac gtttttgcca ctgactgacg
     4081 tggacatgaa tccacccagc ggcactttga tggattcatc agtggatgac gacaacgtat
     4141 accggatcgc cttttttgat agtgctggca tcaaaatcag aaccgagatt aacggtcacg
     4201 tagctacgct tcatggcgtc gcccgggaag ttcttgcctg ttcccacctg gcgaaccatg
     4261 tccggctgcg acgtggtcgg ataagggcgc acgtagatcc ccttcacctt gtctgcggta
     4321 tcaccatctg ccagcggcac gaaaaaaccg tcatcatcgt atttaccagc caggccatag
     4381 gcagcgaagg cgttatcgga tttaaggacc accggttcga cggttaagtc ctgcgggcga
     4441 gagacagccc cggcaatgcc aacaggcatc cggtacagaa atacattatt cattttttac
     4501 cctttacggt ttgcccagaa ttcagcgttt tgtttgttca gggaagcgat actggtcatg
     4561 cccatgttta ggcgctgtgc atcgccggtg gtggcgcggg tgtttcgccc tttggcaacc
     4621 tcagacacgg cattaaacgc catgtcgacc gattgtttcg gtaatttgcg gatatccgca
     4681 tcaccgacta tctggcgaac cagcgttttg tcagcagaag ccagaacctc gcgtttgaac
     4741 gcggtcggtt tcatcttacg gctcagatcg atacccggaa cgataacttc ggcacgccag
     4801 gctgagtcac cagtaatcgt ggtttcctct tcatcgtcct cgccgtcacc ggtcggatta
     4861 tcgtcaggct tattatcgtt atcgcccgtg gcatttcttt ccagcttagc cagcagggct
     4921 ttcagtaatg ttttgaggtc atcatcactg tcgccggttg gatctccgcc catctctggt
     4981 gctttgtccg gtagtggttg ctgcggggac aggttgatgt tgagattaac gccctgcggc
     5041 aaatccccct catctcctgt aaccgatgcg ggagccgact ccaccagttc gttcatggtg
     5101 tcggcatctc ctgtcttgat ggccgcacgc atgcggttcc accagttttt cttttgattt
     5161 gccattgtgt ctctgtctcc aattgcacaa cgatttccgg ctctgccttt agggacaaga
     5221 gccacatggt ttccggtaat atcgacctgc tcggctttac ctggctcggt ctgctcgtac
     5281 tccgcgtcat agccgcacga cacttcgcgc aggccatctt cgataagctg aatggcgctt
     5341 tcgtctttga cgataaggtc agccagcatc aaatcagact gctcacccgt cccgcgccgg
     5401 acattctgga ggtgcccgac agcaagctct ttccagttct cgggatttac cagccgcaca
     5461 ttcccgtttt catcttcagg atgcaggatc gtgatgctca tcccttcgaa tgaggcaagc
     5521 gtagccggat ggaatacctg ctcaggagaa cgcgtgacga ctatttcacc gaacttatcg
     5581 ggtttcagtt ttggcaggtc atcagcacca tagagctgct tacctgttcg tcctatcggc
     5641 acgtctttgc acagcaacga gccgtcagcc aggcggtatc gggtttcccc gagcctggtc
     5701 tcaaaaaagt atttcatggg ttatgctgcc cttttcattg cgaatggaac gcgaacaaac
     5761 acgatctctt tatagcagcg gcagtttggc agctcgcccg cgtggccggt catgccgtcc
     5821 aaagttggag gtgaatccca gcggacgaat ttacctttca tgtgatcgtg ggaatctcgg
     5881 acatcgccat catcggctgt acgccaaata taaccatccg agccaatagc tatcgctcgg
     5941 gccatatcca gcgcgcccgt tgctcttccc agttccgttc gggcaatcag atcggcccta
     6001 gacttagcaa catcgccggt agatgcaatc tctttagcaa actgctccgc ccgaccaccg
     6061 gtgacaacag cctctatcgc tttgttctgg atgtcgtaca ccctgtctgc ggcctcgaga
     6121 ggcagagatt tgatgtactt gacctgttcg gcgatgatgg gttgcatcac ctggcccaca
     6181 ggagcgcttt ccacaagatt gcggagctcg cgactgatgt tcttgctgtg ttgccgccaa
     6241 actttctcgt tctgccgggt taggtccgca gtaaagtttt ccgcgacctt tgtcgcccag
     6301 ggggtgatga tttcactgta acgctccagc gccgcaataa tttccgtgat actgtcattt
     6361 gaaccatcgt agcgaccatt tacgatgtct ccgaccgccc tcgctatcct gcgtaggctg
     6421 gttcgatacc ggatttccgc ctgacggctc ctgcggttcg tcatcaggtt cgccgatgcc
     6481 gggcggcgct tcatcttcgg cattctcgat gtcctcgtcg gtaatggatg ccccgatgcc
     6541 ggttacgtca gaattttctc gcaaatcagt catagcggct ttcagtgtca tcagaccatc
     6601 acccagcgcc gtactgattg cgttggtggt gtttaacgcc accgttgagc gatcgacatc
     6661 agacatttgc cagagcgggt taaactcaaa cgtgaaatcg tccggcagcg gcttgccaag
     6721 ttccgaacga tgcatgatgt ccagtatccg ccgcaccgga agacgtaaac gcctctcctg
     6781 caacgaactg atgcgatcgt aatagttggc aaggtctgca tcaccggtag aaaatccttt
     6841 cggggactgt ccgaacaacc gcaccagtgg gataccaaca gcgccactaa tctgttctgc
     6901 aaactgcgaa aggatgtcat ccagaccact gaagctgtac tgatgggttt caaacttatc
     6961 ccgcgagtcc atgagcgtca tgccttcatt actctggaac tgtcgaatca ggtcgatatt
     7021 cttcagcaac gcttcatacg caggaccacc aagtgcgata agctcgcgta gcttctccac
     7081 gctgtaggta cgcaaatgcg ccttgtagac cagctgcgcc gcgccgacag tagcgctgtc
     7141 gaacgcggta agacgatccc agatacgctc tacaaccgac attccccatt cgttctcggt
     7201 catcttctgc tgaaatggca acgtgacgcc atcaaagcga atcaggcgac tgtgatgaat
     7261 gcgctaggca ggaattcccg ttgctgtggt caccacatcg taaaactcag gtttacccag
     7321 gtccggcccc atatctttaa tgcggcgggt cagtgccggg tcgaccatcc agcggtcgag
     7381 cgggagaatc cccttaaact tgcccttacc gatggtttcg ggtcgcagcg gggtcattgg
     7441 tgcctgcccc tcaatcatga tgaaacccac cgcgccgccg tagaggcgcg accatttcag
     7501 cacgtcattc agcgcatccc agatttgcaa ctcatccagt tgtgattcga gaatgccacg
     7561 atcttttgca tcaatttccg aagtgatgcg aatgcctttg cgggtcatat catccgggat
     7621 agcatcgact gcttcgccga tgatccagga tgaacgatag gaccattcca ccagcatgcg
     7681 gttacgacta gtgaaattag cccggtaggt ggatgctgag tgctggttag gtgtctgcat
     7741 ccctacgcgg gcaataaaat tctcataacc atcagctgtg gcctgcacag ttcgccgcag
     7801 ggcttgtttg tttcgtgcca tcaggcctgt ctccccagct gttcccagat atccagcgat
     7861 gtatcgattg gcgcgaaggc cataatgaat gcatcagcaa cgtttggtga cggtatctcg
     7921 cgttttgcga ggtctttttt actttcgacc atcacacgtc cgttacggtc gaaatcacga
     7981 tgaggtgttg tcagttccag tttcagcttt tcaagcaacg gacaacgaga atctatgctg
     8041 atcagctcat ccacaggata ctgttctccg ttgttaatgg cgttaaacgt atttctgaaa
     8101 cggtcagcca ccagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     8161 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     8221 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     8281 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     8341 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     8401 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     8461 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     8521 gcatccactg cggcttctat ccaagacagt ttgatgattg ccgcatcatc atcagactcc
     8581 ggaacgccct catacacatg tttaaaacca tccggatcac ggcgtcgcgc cgcgtcgata
     8641 accttaagca tggtgtcaga cagaaacgga ttttcgtcat ggttaatttt gcggatgaga
     8701 gtgccttcgg gcggatcaac aacgaagttg cgccagacga aatcagtaac aagtccgggg
     8761 ttgaatatga accagcattc cgaaccctct ttacggatcg ttggctccag aattttccac
     8821 tggtattccg tcagtgcgtg ggcttcttcc agccacaaca catcgatccc ctccagtgac
     8881 ttaatttctt cgatgttgcg ccataatcca taaaaaacaa attccgaacc agtaacccgg
     8941 ttaatgattt tgttgttcag aatacggaaa cggtgccgca gaccaaacct gtctatctga
     9001 attttgagca gggtatacac cgactcttcg attttattct ggatctgacg ggcacaacaa
     9061 aaacgcaggg tgtatttatt cgacagaaat atggcaatgc cagcggcatc ccatgatttt
     9121 gacgatgacc gaccaccata aagcactttg ttacgtgccc gcatagtcca gaaactacgt
     9181 aaagccggat tcagcgtcgg tttggatgtc agagtagaag tcattgaggt cacgctctcc
     9241 attgccatca tcaatacctg catcacggcg aagacgatca gcctccagag acaccttatc
     9301 agtggcggcc ttgcggtagg ctgtatcagc aaatattttt cctaccgtcg caagcgtgcc
     9361 aacgatggac tcaatacgaa ctgtattgcg catcattgct ttctcggcgg cgctgatatt
     9421 ttcaatcagc atcttccttt cctggtcccc attagcatct tccagcgaca ccaaccaccg
     9481 accaatattc tctgcagcga caaggttgtt agcacgaagg cgaaataatt cgtcctcgag
     9541 cgtcaacgct ttagcgtcct ctatcacctc atctttgagc agaaggcgac ggg
//
LOCUS       DADLSA010000143         9578 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.144, whole genome shotgun sequence.
ACCESSION   DADLSA010000143 DADLSA010000000
VERSION     DADLSA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.144"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            58..1341
                     /locus_tag="JN691_003359"
     CDS             58..1341
                     /locus_tag="JN691_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7244713.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAWIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1593..2510
                     /gene="oxyR"
                     /locus_tag="JN691_003360"
     CDS             1593..2510
                     /gene="oxyR"
                     /locus_tag="JN691_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAY7244714.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2493..3893)
                     /gene="sthA"
                     /locus_tag="JN691_003361"
     CDS             complement(2493..3893)
                     /gene="sthA"
                     /locus_tag="JN691_003361"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAY7244715.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            4214..4864
                     /gene="fabR"
                     /locus_tag="JN691_003362"
     CDS             4214..4864
                     /gene="fabR"
                     /locus_tag="JN691_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAY7244716.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4864..5223
                     /locus_tag="JN691_003363"
     CDS             4864..5223
                     /locus_tag="JN691_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAY7244717.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(5263..6363)
                     /gene="trmA"
                     /locus_tag="JN691_003364"
     CDS             complement(5263..6363)
                     /gene="trmA"
                     /locus_tag="JN691_003364"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAY7244718.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6477..6667
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6732..8576
                     /gene="btuB"
                     /locus_tag="JN691_003365"
     CDS             6732..8576
                     /gene="btuB"
                     /locus_tag="JN691_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HAY7244719.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8521..9378
                     /gene="murI"
                     /locus_tag="JN691_003366"
     CDS             8521..9378
                     /gene="murI"
                     /locus_tag="JN691_003366"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY7244720.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLLA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2190 a         2303 c         2568 g         2517 t
ORIGIN      
        1 gcttaattat tttctgaaca gtcatcaaat cgattaattc cgctcaagga gctaaaaatg
       61 gacgttgatg tgtcaaccag tgttgcgggt aataaaccgc aacggattcg tcgtattcag
      121 acagtgaccc tggttttatt atttatggcg tggatcgtta attttctcga ccgttcgtca
      181 ttgagcgtgg caggcgaagc gattcgtggc gagttaggat tatcggcgac agagtttggt
      241 gttttgcttt ctgcattttc tctgtcttat ggtttttcac aactaccttc cggtattttg
      301 ctagaccgct ttggcccacg aatagtttta ggcgcaggct taatattctg gtcattaatg
      361 caggcattaa caggaatggt taattctttt agccacttta ttttaatgcg tatcggtctg
      421 ggtattggcg aagcgccatt tatgcctgcg ggagtaaagt cgatcaccga ctggtatgca
      481 caaaaagaac gcggcacggc gctggggatt tttaactcat ctaccgttat cggtcaggct
      541 atcgcgcctc ctgctcttgt attgatgcag ctggcatggg gctggcggac gatgttcgtt
      601 atcatcggcg tggcagggat tctggttggg atctgttggt acgcgtggta tcgcaaccgg
      661 gcgcagtttg tcctgactga cgaagaacgg acgtatctct ccgcgccggt taaaccgcgt
      721 ccacagctgc aatttagcga gtggctggcg ctgtttaagc atcggacaac ctgggggatg
      781 attttaggtt tctctggcgt caattatacc ggttggctct acattgcgtg gttacccggt
      841 tatttgcaag ccgaacaagg tttcagtctg gcgaaaaccg gttgggtagc ggcgattcct
      901 ttcctcgcgg cggcggtcgg gatgtgggtt aacggtattg tggtcgatcg gctggcgaaa
      961 aaaggctacg acctggcgaa gacgcgtaaa acggctattg tctgcggttt gatgatgtcg
     1021 gcattaggca cgttgctggt cgtgcaatct tcctcgccag ctcaggcggt agcgtttatc
     1081 tcaatggcgc tgttctgcgt gcatttcgcg ggaacgtctg catggggact ggtgcaggtg
     1141 atggtgtcag aaacaaaagt ggcttccatc gccgggattc aaaactttgg cagttttgtt
     1201 tttgcttcct ttgctccgat cgtaaccggt tgggtagtgg ataccacaca ctcgtttaat
     1261 ctggcgctgg ttatcgcggc ctgtgtgacg ttcaccgggg cgctgtgtta cttctttatt
     1321 gtcaaagatc gcattgagta atcgttacta tcaggctctc ttgctgtggg cctgtagaat
     1381 aaaaaaaagc ggatcctgga gatccgcaaa agttcacgtt ggctttagtt attcgagttg
     1441 agaaactctc gaaacgggca gtgacttcaa gggttaaaag aggtgccgct ccgtttctgt
     1501 gagcaattat cagtcagaat gcttgatagg gataatcgtt cattgctatt ctacctatcg
     1561 ccatgaacta tcgtggcgat ggaggatgga taatgaatat tcgtgatctt gagtacctgg
     1621 tggcattggc tgaacaccgc cattttcggc gtgcggcaga ttcctgccac gttagccagc
     1681 cgacgcttag cggccaaatt cgtaagctgg aagatgagct gggcgtgatg ttgctggagc
     1741 ggaccagccg taaagtgttg ttcacccagg cgggaatgct gctggtggat caggcgcgta
     1801 ccgtgctgcg tgaggtgaaa gtccttaaag agatggcaag ccagcagggc gagacgatgt
     1861 ccggaccgct gcatattggt ttgattccta cggttggacc atacctgcta ccgcatatta
     1921 tcccgatgct gcaccagaca tttccaaagc tggaaatgta tctgcatgaa gcacagaccc
     1981 accagttact ggcgcaactg gacagcggca aactcgattg cgtgatcctc gcgctggtga
     2041 aagagagcga agcattcatt gaagtgccgt tgtttgatga gccaatgttg ctggctatct
     2101 atgaagatca cccgtgggcg aaccgcgaat gcgtaccgat ggccgatctg gcaggggaaa
     2161 aactgctgat gctggaagat ggtcactgtt tgcgcgatca ggcaatgggt ttctgctttg
     2221 aagccggggc ggatgaagat acacacttcc gcgcgaccag cctggaaact ctgcgcaaca
     2281 tggtggcggc aggtagcggg atcactttat tgccagcgct ggctgtgccg ccggagcgca
     2341 aacgcgatgg ggttgtttat ctgccatgca ttaagccgga accacgccgc actattggcc
     2401 tggtttatcg tcctggctca ccgctgcgca gccgctatga gcagctggca gaggccatcc
     2461 gcgcaagaat ggatggccat ttcgataaag tgttaaaaca ggcggtttaa accgtttaac
     2521 gcagctaccc gataggcttc cgccatcgtc gggtagttaa aggtggtgtt gacgaagtac
     2581 tcaatagtgt tgccgccacc tttctgttcc ataatcgcct gaccgatatg aataatttcg
     2641 gcagcgcgct cgccaaagca gtgaataccc agaatctctt ttgtttcccg atggaacaaa
     2701 attttcagcg tgcccacgtt catgccgacg atttgtgcac gtgccagatg tttgaactgg
     2761 gcgcggccca cttcatacgg cactttcatc gcggtcagct gctgttcggt tttgcccaca
     2821 gagctgattt ccgggatggt gtaaatgccg gtagggatat cttcaatcag atgtgcggtg
     2881 gcttcgcctt ttaccagcgc ctgcgcggca atgcgcccct ggtcataggc cgctgatgcc
     2941 aggctcggat aaccaatcac gtcgcccacc gcgtaaacgt gcggctgtgc ggtctgatac
     3001 atgctgttga ccttcagctg tccgcgactg tcagtttcca gcccaatgtt ctgtaacgcc
     3061 agcgaatcgg tattaccggt acgaccgttg gcatagagca ggcagtcagc tttcagtttt
     3121 ttacccgact tcagatgcat gatcacaccg tcgtcacagc tttcgatctt ctcgtactct
     3181 tcgttgtgac gaatcactac gccactgttc cagaagtgat aggagagaga atctgacatc
     3241 tcttgatcga gaaatgccag caggcgatcg cgggtgttga tcagatccac ttttacatcc
     3301 ataccgcgga agatcgacgc atattcacag ccgatcactc cagcaccata gataagtaca
     3361 tggcgcggtt cgtggtgcat gctaagaatt gagtcgctgt cgtaaatgcg tggatgggtg
     3421 aaatcaacat ctgttggatg atatggacga gagccgcagg caataacaaa tttttcagcg
     3481 gttagtgttt caacgctgcc gtccgggcaa tccagcgcca acgtatgctc gtcaacaaag
     3541 cgagcgttcc cctgcaatat ttcacagtga ttacgttcgt aaaatccctg acgcatgcgc
     3601 gtttgttgat taatcacgtt atcggcatgg ttaaggatat cggcaaaaga agagcggagc
     3661 agtcgggaat ggtcgctgta tagtgggttt tgattgaatt ctataatgcg gctgacggcg
     3721 tgacggagag ctttcgacgg gatggtgccc cagtgggtgc aaccgccgcc aacattttga
     3781 taacgctcga taactgcgac gcgcgcacct tgcttaacca ggcccattgc agcgccttcg
     3841 ccgccggggc cggaacctat tactatggca tcgtaatcgt aggaatgtgg catggtaggg
     3901 cttacctgtt cttatacata aaagcaacag aatggtaaca ttctatcgcg ggtaagccaa
     3961 ttgatccccg tcatttatct ggctatatcc tgagcggcct ttgctttgtc tgtttcttac
     4021 ttttgccctg acgttttatt ggatttttat cgacgatact ctccgtttaa gcggcaggtt
     4081 tccgctgtac gtaaaagaac cggccagtaa atatgtttta ttgcgttacc gttcattcac
     4141 aatactggag caatccagta tgttcattct ctggtatagt gccagcagta cttttggcaa
     4201 ggattcagac atcgtgatgg gcgtaagagc gcaacaaaaa gaaaaaaccc gccgttcgct
     4261 ggtggaagcc gcatttagcc aattaagtgc tgaacgcagc ttcgccagcc tgagtttgcg
     4321 tgaagtggcg cgtgaagcgg gcattgctcc cacctctttt tatcggcatt tccgcgacgt
     4381 agacgaactg ggtctgacca tggttgatga gagcggttta atgctacgcc aactcatgcg
     4441 ccaggcgcgt cagcgtatcg ccaaaggcgg gagtgtgatc cgtacctcgg tctccacatt
     4501 tatggagttc atcggtaata atcctaacgc cttccggtta ttattgcggg aacgctccgg
     4561 cacctccgct gcgtttcgtg ccgccgttgc gcgtgaaatt cagcacttca ttgcggaact
     4621 tgcggactat ctggaactcg aaaaccatat gccgcgtgcg tttactgaag cgcaagccga
     4681 agcaatggtg acaattgtct tcagtgcggg tgccgaggcg ttggacgtcg gcgtcgaaca
     4741 acgtcggcaa ttagaagagc gactggtact gcaactgcga atgatttcga aaggggctta
     4801 ttactggtat cgccgtgaac aagagaaaac cgcaattatt ccgggaaatg tgaaggacga
     4861 gtaatgaaac aagcaaatca agatagaggt acgctgctgc tggcgttggt tgctggctta
     4921 tcgattaatg gtactttcgc agcgctgttt agctccattg tgccattttc tgtattcccg
     4981 attatttccc tggtgctgac ggtttactgc ctgcatcaac gttatcttaa tcgcaccatg
     5041 tcggtaggct tgccgggtct ggcagctgcc tgttttattc tcggcgtact gctgtacagc
     5101 acggtagttc gtgcggaata tccggatatc ggctctaact tcttcccggc agtactctcc
     5161 gtcattatgg tgttctggat tggcgcgaag atgcgtaacc gtaagcagga agttgctgag
     5221 taatcggtat catgccggat gctggcgcat ccggcatggg ttttacttcg cggtcagtaa
     5281 tacgccgcac tccatatggt gcgtgtaggg gaactgatca aacagagcca gacgttcgac
     5341 cttgtgcgtc tggcttaatg tttccagatt tttgcataac gtttccggat tgcaggagat
     5401 atacagaata cgtggatacg cctgcaccat tttctcggtt tcactgtcca gaccgctgcg
     5461 cggtgggtcg acaaaaatgg tttcgaactg atagctcttt aagtcgatcc cttgcaggcg
     5521 gttaaactcg cgcacaccat tcatcgcctg agtaaattct tctgccgcca tacgaataat
     5581 ttgtacgtta tcaatatggt tagcggcaat gttgtattgc gcggcagcga ctgacggttt
     5641 ggcgatttcg gtggctaata cccgatcaaa attgcgcgcc agcgctaatg aaaagttacc
     5701 gttgccgcag tacagctcca gtaaatcgcc ttttgagcct ttggttacgt ccagcgccca
     5761 ttccagcatc tgaatattca tcgccgcgtt cggctgggta aagctgtttt ctacctgacg
     5821 atagatcatc tctttccctg cgaccggcag acgctcgtcg atgtaatctt gatccagcgc
     5881 gattttggtt ttcgttgccc gaccaatcag atgcacattc agattctgcg cgcgcagtgc
     5941 atcgcgcagt gcttccgctt cctgacgcca ctcatcatcc agctttttat ggtacagcag
     6001 ggaaaccacc gcctgattac tcagcgtggt gaggtaatca atctggaaca acttgtggcg
     6061 cagaacggga ttattacgca cacccgcaat catcgccgtc atcaactggt tgatgagttc
     6121 actggcggcg gggaagctat ccacgcggat gcggcttttg gtttgttgat cgaaaatgat
     6181 gtgatacagg tcatcgccat cgtgccagat gcggaactcc gcgcgcatcc ggtaatgact
     6241 gaccggcgag cgaaacactt ccggaaccag gtcagaaaac ggtgccatca tactttgcaa
     6301 acgtaccact ttttcggcta actgcgcttc atactgttct gttggaaggt gttcgggggt
     6361 catgatgtat cctgaaaaat taaagtacgc ggcgattgta gggattgctc atcagatgtc
     6421 cagatcttga tgaattccta tttgtgagct acgtctggac agtaacttgt tacaacctgt
     6481 agcatccact tgccggtcct gtgagttaat agggaatcca gtgcgaatct ggagctgacg
     6541 cgcagcggta aggaaaggtg cgatgattgc gttatgcgga cactgccatc cggtgggaag
     6601 tcatcatctc ttagtatctt agatacccct ccaagcccga agacctgccg gccaacgtcg
     6661 catctggttc tcatcatcgc gtaatattga tgaaacctgc ggcatccttc ttctattgtg
     6721 gatgctttac aatgattaaa aaagcttcgc tgctgacggc gtgttccgtc acggcatttt
     6781 ccgcttgggc acaggatacc agcccggata ctctcgtcgt tactgctatc cgttttgaac
     6841 agccgcgcag cactgtgctt gcaccaacca ccgttgtgac ccgtcaggat atcgaccgct
     6901 ggcagtcgac ctcggtcaat gatgtgctgc gccgtcttcc gggcgtcgat atcacccaaa
     6961 acggcggttc aggtcagctc tcatctattt ttattcgcgg tacaaatgcc agtcatgtgc
     7021 tggtgttaat tgatggcgtc cgcctgaatc tagcgggggt gagtggttct gccgacctta
     7081 gccagttccc tattgcgctt gtccagcgta ttgaatatat ccgtggaccg cgctccgctg
     7141 tttatggttc cgatgcaata ggcggggtgg tgaatatcat cacgacgcgc gatgaacccg
     7201 gaacggaaat ttcagcaggg tggggaagca atagttatca gaactatgat gtctctacgc
     7261 agcaacaact gggggataaa acacgggtaa cgctgttggg cgattatgcc catactcatg
     7321 gttatgatgt tgttgcctat ggtaataccg gaacgcaagc gcagacagat aacgatggtt
     7381 ttttaagtaa aacgctttat ggggcgctgg agcataactt tactgatgcc tggagcggct
     7441 ttgttcgcgg ctatggctat gataaccgta ccaattatga cgcgtattat tctcccggtt
     7501 caccgttgct cgatacccgt aaactctata gccaaagttg ggacgccggg ctgcgctata
     7561 acggcgaact gattaaatca caactcatta ccagctatag ccatagcaaa gattacaact
     7621 acgatcccca ttatggtcgt tatgattcgt cggcgacgct cgatgagatg aagcaataca
     7681 ccgtccagtg ggcaaacaat gtcatcgttg gtcacggtag tattggtgcg ggtgtcgact
     7741 ggcagaaaca gactacgacg ccgggtacag gttatgttga ggatggatat gatcaacgta
     7801 ataccggcat ctatctgacc gggctgcaac aagtcggcga ttttaccttt gaaggcgcag
     7861 cacgcagcga cgataactca cagtttggtc gtcatggaac ctggcaaacc agcgccggtt
     7921 gggaattcat cgaaggttat cgcttcattg cttcctacgg gacatcttat aaggcaccaa
     7981 atctggggca actgtatggc ttctacggaa atccgaatct ggacccggag aaaagcaaac
     8041 agtgggaagg cgcgtttgaa ggcttaaccg ctggggtgaa ctggcgtatt tccggatatc
     8101 gtaacgatgt cagtgacttg atcgattatg atgatcacac cctgaaatat tacaacgaag
     8161 ggaaagcgcg gattaagggc gtcgaggcga cagccaattt tgataccgga ccactgacac
     8221 atactgtgag ttatgattat gtcgatgcgc gcaatgcgat taccgacacg ccgttgttac
     8281 gccgtgctaa acagcaggtg aaataccagc tcgactggca gttgtatgac ttcgactggg
     8341 gtattactta tcagtattta ggcactcgct atgataagga ttactcatct tatccttatc
     8401 aaaccgttaa aatgggcggt gtgagcttgt gggatcttgc ggttgcgtat ccggtcacct
     8461 ctcacctgac agttcgtggt aaaatagcca acctgttcga caaagattat gagacagtct
     8521 atggctacca aactgcagga cgggaataca ccttgtctgg cagctacacc ttctgaacca
     8581 cgtcccaccg tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta tgacgagatc
     8641 cggcatctct taccggatct ccattacatt tatgctttcg ataacgtcgc tttcccgtat
     8701 ggcgaaaaaa gcgaagcgtt tattgttgag cgagtggtgg caattgtcac cgcggtgcaa
     8761 gaacgttatc cccttgcgct ggctgtggtc gcttgcaaca ccgccagtac cgtttcactt
     8821 cttgcattac gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc gattaaacct
     8881 gctgcacgtc tgacggcaaa tggcattgtc ggattactgg caacccgcgg aacagttaaa
     8941 cgttcttata ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat agaaatgctg
     9001 ggctcggcag agatggttga gttggctgaa gcgaagctgc atggcgaaga tgtttctctg
     9061 gatgcactaa aacgtatcct acgcccgtgg ttaagaatga aagagccgcc agataccgtt
     9121 gtattgggtt gcacccattt ccctctacta caagaagaac tgttacaagt gctgccagag
     9181 ggaacccggc tggtggattc tggcgcagcg attgctcgcc gaacggcctg gttgttagaa
     9241 catgaagccc cggatgcaaa atctgccgat gcgaatattg ccttttgtat ggcaatgacg
     9301 ccagaagctg aacaattatt gcccgtttta cagcgttacg gcttcgaaac gctcgaaaaa
     9361 ctggcagttt taggctgatt tggttgaatg ttgcgcggtc ggaaaattat tttaaatttc
     9421 ctcttgtcag gccggaataa ctccctataa tgcgccacca ctgacacgga acaacggcaa
     9481 acacgccgac gggtcagcgg ggttctcctg agaattccgg cagagaaagc aaaaacaaat
     9541 gcttgactct gtagcgggaa agtgtattat gcacaccc
//
LOCUS       DADLSA010000144         9529 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.145, whole genome shotgun sequence.
ACCESSION   DADLSA010000144 DADLSA010000000
VERSION     DADLSA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.145"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            20..157
                     /gene="sra"
                     /locus_tag="JN691_003367"
     CDS             20..157
                     /gene="sra"
                     /locus_tag="JN691_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HAY7244721.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            308..2005
                     /locus_tag="JN691_003368"
     CDS             308..2005
                     /locus_tag="JN691_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAY7244722.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHSPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAAKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2139..3149
                     /gene="adhP"
                     /locus_tag="JN691_003369"
     CDS             2139..3149
                     /gene="adhP"
                     /locus_tag="JN691_003369"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HAY7244723.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3295..3579
                     /locus_tag="JN691_003370"
     CDS             3295..3579
                     /locus_tag="JN691_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY7244724.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTSEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3807..4460)
                     /gene="fdnI"
                     /locus_tag="JN691_003371"
     CDS             complement(3807..4460)
                     /gene="fdnI"
                     /locus_tag="JN691_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HAY7244725.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4453..5337)
                     /gene="fdxH"
                     /locus_tag="JN691_003372"
     CDS             complement(4453..5337)
                     /gene="fdxH"
                     /locus_tag="JN691_003372"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY7244726.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5350..8397)
                     /gene="fdnG"
                     /locus_tag="JN691_003373"
     CDS             complement(5350..8397)
                     /gene="fdnG"
                     /locus_tag="JN691_003373"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7810..7812),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY7244727.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8629..9510
                     /gene="yddG"
                     /locus_tag="JN691_003374"
     CDS             8629..9510
                     /gene="yddG"
                     /locus_tag="JN691_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAY7244728.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGVHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSALLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2290 a         2514 c         2420 g         2305 t
ORIGIN      
        1 acaggattga ggagttaaaa tgaaatcgaa ccgtcaggca cgtcatattc ttggactgga
       61 ccataaaatt tctaaccagc gcaaaatagt taccgaaggc gacaaatcca gcgtggtaaa
      121 taacccaacc ggcagaaaac gccccgctga aaagtaattc ataaccatca gtcctcaatg
      181 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
      241 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      301 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      361 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      421 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      481 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      541 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      601 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      661 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      721 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      781 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      841 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      901 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      961 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
     1021 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
     1081 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
     1141 agtcggcaca ctgatcgcag ccagtcgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1201 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1261 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1321 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1381 gcgcgaaaac ctcagtgatt gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1441 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1501 agagatcatc cgtgagatgc ataaacactc tccgcgtccg atcgtgatgc cgctgtctaa
     1561 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1621 gctggtcgcc actggcagcc cgtttaatcc agtggtatgg aaagataaaa tctaccctat
     1681 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1741 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1801 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1861 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggccaa
     1921 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1981 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     2041 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     2101 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     2161 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2221 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2281 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2341 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2401 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2461 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc gccgattacg
     2521 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2581 ttaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2641 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcaa
     2701 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atgggtgcag
     2761 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2821 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2881 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2941 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     3001 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     3061 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     3121 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3181 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3241 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3301 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3361 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3421 accggaaaag cagctttgac gtcagaaatg gcaattaaac tttccgtggt gatcggcagt
     3481 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3541 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3601 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3661 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3721 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3781 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3841 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3901 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3961 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     4021 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     4081 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     4141 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4201 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4261 gcgacgaaaa tcgcaatgcc gaagaacggg tgcaaaatgc gtcccatctg cggcgtaccg
     4321 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4381 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4441 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4501 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4561 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4621 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacat gcgtaccacc
     4681 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4741 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4801 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4861 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4921 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4981 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     5041 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     5101 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     5161 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5221 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5281 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5341 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5401 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5461 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5521 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5581 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5641 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5701 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5761 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5821 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5881 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5941 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     6001 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     6061 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     6121 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6181 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6241 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6301 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6361 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6421 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6481 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6541 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6601 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6661 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6721 cgggttaaag ccctggcaga aataaccggt gactttgcct tcatccatca tgttgaaata
     6781 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6841 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6901 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6961 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     7021 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     7081 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     7141 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7201 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7261 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7321 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7381 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7441 ttcgaaagca aaatcatcac gcaccagcag gctggcgttg gtgtaatgtt taacgtattc
     7501 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaacg ccagacagga acgtaatgtc
     7561 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7621 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7681 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7741 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7801 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7861 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7921 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7981 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     8041 gtcagaacct ggcgcacgat attccgggta gcgcagacgg ttttcactgt tgacgtaatc
     8101 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     8161 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8221 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8281 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8341 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8401 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8461 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8521 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8581 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8641 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8701 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8761 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8821 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg
     8881 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8941 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     9001 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tggggtacat
     9061 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     9121 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9181 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9241 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9301 accttaggat ttgcttatgc tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9361 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9421 agcgccctgc tatcgttctc gttctggcaa ggcgcgctaa tggtctgcgg cggttccctg
     9481 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacg
//
LOCUS       DADLSA010000145         9462 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.146, whole genome shotgun sequence.
ACCESSION   DADLSA010000145 DADLSA010000000
VERSION     DADLSA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.146"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            100..252
                     /locus_tag="JN691_003375"
     CDS             100..252
                     /locus_tag="JN691_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7244729.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            603..755
                     /locus_tag="JN691_003376"
     CDS             603..755
                     /locus_tag="JN691_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7244730.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(877..1497)
                     /gene="entD"
                     /locus_tag="JN691_003377"
     CDS             complement(877..1497)
                     /gene="entD"
                     /locus_tag="JN691_003377"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HAY7244731.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1654..3894)
                     /gene="fepA"
                     /locus_tag="JN691_003378"
     CDS             complement(1654..3894)
                     /gene="fepA"
                     /locus_tag="JN691_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HAY7244732.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADARDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDMMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4137..5339
                     /gene="fes"
                     /locus_tag="JN691_003379"
     CDS             4137..5339
                     /gene="fes"
                     /locus_tag="JN691_003379"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HAY7244733.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5342..5560
                     /locus_tag="JN691_003380"
     CDS             5342..5560
                     /locus_tag="JN691_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HAY7244734.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5557..9438
                     /gene="entF"
                     /locus_tag="JN691_003381"
     CDS             5557..9438
                     /gene="entF"
                     /locus_tag="JN691_003381"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HAY7244735.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQWYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
BASE COUNT         2054 a         2488 c         2611 g         2309 t
ORIGIN      
        1 taactgtcca actttttggg gtcagtacac cctagggctg ccactccaaa ccagacaatt
       61 tggatggtag tcccttcttc gcatggaggc aatataaaca tgctgacgaa atatgccctt
      121 gtggcagtca tagtgctgtg tttgacggta ctgggattta cgcttctggt cggagactcg
      181 ctgtgtgagt ttacggtgaa ggaacgtaat attgagttca gggctgttct cgcttacgaa
      241 ccgaagaagt agccgttgtg cggggagtaa tccccgcata tccggttgtc aggtcaggat
      301 ggtaaggcac ctgctttaca ctttcgcccg cggtcagtga tggctgcggg cgatttcgtt
      361 ttaagagcaa tgatgagttt tgccgacaga agtccgaaac aggactcact gtataaataa
      421 acagctattt tgctaaggaa gggtaagata acggcgggtg cctgaagctt tccggtttca
      481 ggttaactct gaggtttgga aagatgaagc cccaagagat atttctatca atcctggggc
      541 tgccactccg aacctcaaca actcggatgg tagtcccttc ttcgcatgga ggcaatataa
      601 acatgctgac gaaatatgcc cttgtggcag tcatagtgct gtgtttgacg gtgctgggat
      661 ttacgcttct ggtcggagac tcgctgtgtg agttcaccgt aaaggaacgt aatattgagt
      721 ttaaggctgt tctcgcttac gaaccgaaga agtagccgtt gtgcggggag taatccccgc
      781 atatccggtt gtcaggtcag agtggtaagg cacctgcttt acactttcgc ccgcagtcag
      841 tgatggctgc gggcgaatcg taccagatgt tgtcaattaa tcgtgttggc acagagttat
      901 gactattttt tcttttatct gccagtgcac agcaaacatc tcattctcac gatgaatgat
      961 gacctgctgt ttattccagc taattatctg atagtccaga aaacctgcat cagtttggat
     1021 ctcacttgcc ttaaatgcgc tctctttggc ggaaaatgcc agtgtcagcg ccagagaaaa
     1081 ggctaaaccg cagtctgcga gtcgctcgtg ttctgctggt gtaataatgt tgtctgtcaa
     1141 ttcggtcgct gtttgcgcag aaaaaatttc ttcgatatca acgccaatcg gttgacggga
     1201 aaccacggcc aatgccgtag ccccgcagtg gctaatactg ccgtatacct ccgcaggcca
     1261 caccggttgg cgtagctcgc cgattgcggg cacacattta tagccatatt cccgcaaggc
     1321 ataaacagca gcgatccgtc cggctaaatg ctctgtttta cgtttacgtc cagcgtgttg
     1381 cagttgtgcg tagtgcggca gccagagtaa atcctgctca caaaaattcg ccggatcgaa
     1441 ctcaacaaaa tgcagcgtat gtccggcaaa ggggagggag gtatgcgtag ttttcatatc
     1501 gaccatattc gagactgatg acaaacgcaa aactgcctga tgcgctacgc ttatcaggcc
     1561 tacgtgatcc tgcaatatat tgaattggca agatctttgt aggccgcatc cggcgtgaac
     1621 gacgcgcact ttgtcaacaa tctgacgtta gcatcagaag tgagtattaa tgctcatata
     1681 ccacgtacgt cccggctcgt tataggtata cgcgccggca ccggcgatat agttggctcc
     1741 tgccagatcg cccgtggtct gggcattacc tgcacgccac aaacgtttgt cgaacaggtt
     1801 gtccacgccg ccggtcagac tgacattctt cgtcacatcc caggtcgcgc tcagacccac
     1861 aatgctgtaa ggactgattt ctttggtttc cgggccaacc gctggctgac ctttatagtt
     1921 gtacttcttc ggctgctgct tgccgtacca ggtgaaggtc gtttgcatcg acaaatcttc
     1981 ccgtgcctgc cagctcagcg ttgagttcaa cgtgtactcc gggatgatcg acaaacggtc
     2041 gcccgtggtt ttgttttcac tcttcagcat ataagtgatg ttattggtcc acatcaccgt
     2101 ttcgctaact ggtacgttta acgatccttc cagaccttca accactgctt tcggcacgtt
     2161 atcccactga tagagatcag tgccgactgc gttttgccct acagccacat agcccgcttc
     2221 aatcttattg cgataatcgt tacggaacca ggtgacgccc gccagccagc cgtcgcgttt
     2281 gaactccaga ccaatctctt tgttgatgct ggtttctgct ttcaggtcat cgttaccttg
     2341 cagatagcag ccgcccgcgc tggcatagca gccctgacct ttactgtaga gaatgtagtt
     2401 cgggttagtc tggtacaggc tcggcgcttt ataggcgcgg gcaatgccta ttttcagcgt
     2461 gaagtcatcg cctaaacctt gcgatatgtt cagcgccggg ctccagttat tgccgacaat
     2521 actgtgatga tcgaaacgca gccccggcgt tacgatggtg ctgtcagtca gctccatgtt
     2581 gttttcggca aacagcgaga aaatttctgc ttttgaatac gggctacggt cggtggcact
     2641 cacgccatca atagcgccac cggtattcgt tccggtcagt gcctgggtgt tggaacttaa
     2701 gtccttcatc cgttgctgat tccactccgt acccagcgtc agcgtctggt taacgaggaa
     2761 atcaatcggc aggttaactt cgctgtgcag catcatgtca tcgagatcga tatcgacgaa
     2821 atcctgtgtc gctttttcgt taaatttccc ttcggtaccg cccgccagac cttccggaat
     2881 acgcgagtta cgggtgtgtt catactgcac ccagttgctg gtggtcacgc cgttatccca
     2941 gccaccgttc caggtcagcg cgtagttctg gcgatacagg cggttggttt catcgccata
     3001 tttcgagcga gtgtaagcgt cagagttggt gttctgggta tcgcccgcat acaggttacc
     3061 ctggcggctg taacccgctt ccagttccag cgattgcaat ggtgcgaagt cccagcgcac
     3121 cacgccgtta atatctttat tgatcacccc ttcgcgcccg gctggtaacg tcgtggcata
     3181 cgttccggca cgcgcggact gatggccctg gttgatatcc ctcgcgtcag cctgagtttt
     3241 gtcgaggttg ccatacaagc ggaagctgaa ttcgtcgccc agcggaccgg tcaggctaaa
     3301 gttagtgcgt ttggtggcac cttcctcttt atgttctggc gcattgaaat atgcgtccca
     3361 ggagccgtgc cactcgccgc tgcctttttt ggtaatgatg ttaaccacgc cgcccgccgc
     3421 gccgttgcca taacgcgcag ctgccggacc acgcagaact tcaatacgtt caatcatttc
     3481 aggtggcacc caggaggtat caccacgggt atcgcgctcg ccacgccagc cctgacgcac
     3541 cgagttacgg ctgcttaccg gcttgccgtc aatcaaaatc agcgtgtttt ccggacccat
     3601 accgcgaata tcaatctgtc ggttattccc acgctgacca ctggtggagt taccggtcag
     3661 gttaacgcct ggcatggtac ggatgatctc cgacacatcg cgggcaaccg ggtttttgcg
     3721 gatttcatct gcggtgatgg tcgaaacgcc aggtgcctgc aagttctgct cggcggcggt
     3781 aacgacaatg gtatcgtcat gtgaaacagg agtatcggtc ggctcttgtg cctgcgctac
     3841 cccataaatc cccagattga ccaacaaggc cagggaatga atcttcttgt tcattgtttt
     3901 attcctgcat ttttgccacg aattgcaact gtcgggcatg gtcgtcatca acacgacgca
     3961 tcccgttacc gcgaaaacct ttgatcctga aagacacgca gtgcagttgg ttaattaatg
     4021 tccgcgcttc ccacggcgcg ccattacgct attgtaaatg caaatagtta tcaataatat
     4081 tatcaatata tttctgcaat caatgaaaaa ttgcacagta aacatggggt tatggtgtga
     4141 cggcgttaaa agtaggaagt gagagctggt ggcagtcgaa acatggcccg gaatggcagc
     4201 gtctgaatga cgaaatgttt gaggtcactt tctggtggcg tgatccccaa ggttctgaag
     4261 aatactcgac gataaagcgc gtatgggtct acatcactgg tgtgaccgat catcatcaga
     4321 acagccagcc ccagtcgatg cagcgaattg caggtactga tgtctggcag tggacgacac
     4381 aactcaatgc caactggcgc ggcagctact gctttattcc caccgaacgc gatgacattt
     4441 tttctgcacc atcccccgat cgcctcgaat tacgtgaagg ctggcgaaaa ctattacccc
     4501 aggcaatagc cgatccgctg aatccacaaa gctggaaagg cgggcgaggg cacgctgttt
     4561 ctgcactcga aatgccgcaa gcgcctctgc aaccgggatg ggattgtccg caagcgccag
     4621 aaataccagc caaagaaatt atctggaaaa gtgaacggtt gaaaaattca cgtcgtgtat
     4681 ggatttttac caccggcgat gtaacagcag aagaacgccc gctggcagtt ttgctcgatg
     4741 gcgaattttg ggcgcaaagt atgcccgtct ggcctgtgct gacttcgctg acccatcgtc
     4801 agcaacttcc tcccgccgtg tatgtgttga tcgacgctat cgacaccacg caccgcgccc
     4861 acgaactgcc gtgtaatgcg gatttctggc tggcagtaca gcaagagtta ttacccctgg
     4921 tgaaagttat tgcccctttt agcgatcgcg ccgatcgcac cgtggtcgcc gggcagagtt
     4981 ttggtgggct gtccgcgctg tatgccggac tgcactggcc tgaacgcttt ggctgtgtat
     5041 taagccagtc aggatcgtac tggtggccgc atcggggcgg gcagcaagag ggcgtgttac
     5101 ttgaaaagct aaaagctggt gaagttagcg ccgaaggtct gcgcattgtg ctggaagcgg
     5161 gtattcgcga gccgatgatc atgcgggcca atcaggcgct gtatgcgcaa ttacacccta
     5221 taaaagaatc cattttctgg cgtcaggttg acggcggaca tgatgcgctt tgttggcgcg
     5281 gtggcttgat gcaggggtta atcgacctct ggcaaccact tttccatgac aggagttgaa
     5341 tatggcattc agtaatccct tcgatgatcc acagggagcg ttttacattt tgcgcaatgc
     5401 gcaggggcaa ttcagtctgt ggccgcaaca atgcgcctta ccggcaggct gggacattgt
     5461 gtgtcagccg cagtcacaag agtcctgcca gcagtggctt gaagcccact ggcgtactct
     5521 gacaccggcg aatttcaccc agttgcagga ggcacaatga gccagcattt acctttggtc
     5581 gccgcacaac ccggcatctg gatggcagaa aaactgtcgg atttaccctc cgcctggagc
     5641 gtggcgcatt acgttgagtt aaccggagag gttgatgcgc cattactggc ccgcgcggtg
     5701 gttgccggat tagcgcaagc agatactttg cggatgcgtt ttacggaaga taacggcgaa
     5761 gtctggcaat gggtcgatga tgcgctgata ttcgaactgc cagaaattat cgacctgcga
     5821 accaatattg atccgcacgg tactgcgcag gcattaatgc tggcggattt gcaacaagat
     5881 ttgcgcgtcg atagcggtaa accactggtc tttcatcaac tgatacaggt ggcagataac
     5941 cgctggtact ggtatcagcg ttatcaccat ttactggtcg atggcttcag ttttccggcc
     6001 attacccgcc agatcgccaa catttactgc gcattgctgc gtggcgaacc aacgcctgct
     6061 tcgccgttta cgcctttcgc tgatgtagtg gaagagtacc agcaataccg cgaaagcgaa
     6121 gcctggcagc gtgatgcggc attctgggtg gaacagcgtc gccaactgcc accgcccgcg
     6181 tcactttctc cggcaccttt agcggggcgc agcgcctcgg cagatatttt gcgtctgaaa
     6241 atggaattta ccgacgggga attccgccag ctggctacgc aactttcagg tgtgcagcgt
     6301 accgatttag cccttgcgct gacggctttg tggctggggc gattgtgcaa tcgcatggac
     6361 tacgccgccg gatttatctt tatgcgtcga ctgggctcgg cggcgctgac ggctaccgga
     6421 cccgtgctca acgttttgcc gctgggtatt catattgcgg cgcaagaaac gctgccggaa
     6481 ctggcaaccc gactggcagc acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc
     6541 gaacaaattg tccgtgacag cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta
     6601 ctcaatatta aggtatttga ttaccaactg gatattcctg gtgttcaggc gcaaacccat
     6661 accctggcaa ccggtccggt taatgacctt gaactggccc tgttcccgga tgaacacggt
     6721 gatttgagta ttgagatcct cgccaataaa cagcgttacg atgagccaac gttaatccag
     6781 catgctgaac gcctgaaaat gctgattgcc cagttcgccg cggatccggc gctgttgtgc
     6841 ggcgatgtcg atattatgct gccaggtgag tatgcgcagc tggcgcagat caacgccact
     6901 caggttgaga ttccagaaac cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca
     6961 ccggatgctc cggcgctggc agatgcgcgt tacctgttca gctatcggga aatgcgcgag
     7021 caggtggtgg cgctggcgaa tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg
     7081 gcggtggcac taccgcgctc ggtctttttg accctggcac tccatgcaat agttgaagct
     7141 ggcgcggcct ggctaccgct ggataccggc tatccggacg atcgcctgaa aatgatgctg
     7201 gaagatgcgc gtccgtcgct gttaattacc accgacgatc aactgccgcg ctttagcgat
     7261 gttcccaatt taaccaacct ttgctataac gccccgctta ccccccaggg cagtgcgccg
     7321 ctgcaacttt cacaaccgca ccacacggct tatatcatct ttacctctgg ctccaccggc
     7381 aggccgaaag gggtaatggt cgggcagacg gctatcgtca accgcctgtt gtggatgcaa
     7441 aatcattatc cgctcacagg cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat
     7501 gtctcggtgt gggagttttt ctggccgttt attgccgggg ctaaactggt gatggctgaa
     7561 ccggaagcgc accgcgaccc gctcgctatg cagcaattct ttgccgaata tggcgtaacg
     7621 accacgcact ttgtgccgtc gatgctggca gcatttgttg cctcgctgac tccgcaaacc
     7681 gctcgccaga gttgcgtgac gttgaaacag gttttctgta gtggtgaggc cttaccggct
     7741 gatttatgcc gcgaatggca acagttaacc ggcgcgccgt tgcataacct gtatggcccg
     7801 acggaagcgg cggtagatgt cagctggtat ccggcttttg gcgaggaact ggcacaggtg
     7861 cgcggcagca gtgtgccgat tggttatccg gtatggaata cgggtctgcg tattcttgat
     7921 gcgatgatgc atccggtgcc gccgggtgtg gcgggtgatc tctatctcac cggtattcaa
     7981 ctggcgcagt ggtatcttgg acgacccgat ctgaccgcca gccgctttat tgccgatcct
     8041 tttgctcctg gtgaacggat gtatcgtacc ggagacgttg cccgctggct ggataacggc
     8101 gcggtggagt acctcgggcg cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa
     8161 ctgggcgaaa tcgatcgcgt gatgcaggcg ctgccggatg tcaaacaagc cgttacccac
     8221 gcctgtgtga ttaaccaggc ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat
     8281 ctggtatcgc aatcgggcct gccgttggat accagcgcat tgcaggcgca gcttcgcgaa
     8341 acattgccgc cgcatatggt gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc
     8401 gccaacggca agctggatcg caaagcctta ccgttgcctg aactgaaggc acaagcgcca
     8461 gggcgtgcgc cgaaagcggg cagtgaaacg attatcgccg cggcattcgc gtcgttgctg
     8521 ggttgtgacg tgcaggatgc cgatgctgat ttctttgcgc tgggcggtca ttcgctactg
     8581 gcaatgaaac tggcagcgca gttaagtcga cagtttgctc gtcaggtgac gccggggcag
     8641 gtgatggtcg cgtcaaccgt cgccaaactg gcaacgatta ttgatggtga agaggacagc
     8701 tcccggcgca tgggattcga aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg
     8761 ttttgtttcc atccggcgtc cggttttgcc tggcagttta gcgtgctctc gcgttatctc
     8821 gatccacaat ggtcgattat tggcattcag tcgccgcgcc ctcatggacc catgcagaca
     8881 gcgacaaacc tggatgaagt ctgcgaagcg catctggcaa cgctacttga gcaacaaccg
     8941 cacggccctt attacctgct gggttattca cttggcggta cgctggcgca gggcattgcg
     9001 gcgcggctgc gtgcccgtgg cgaacaggtg gcatttcttg gtttgctgga tacctggccg
     9061 ccagagacgc aaaactggca ggaaaaagag gctaatggtc tggacccgga agtgctggcg
     9121 gagattaacc gcgaacgcga ggccttcctg gcggcacagc agggaagtac ttcaacggag
     9181 ttgtttacca ccattgaagg caactacgct gatgctgtgc gcctgctgac gactgctcat
     9241 agcgtaccgt ttgatggtaa agcgacgctg tttgttgctg aacgcacgct tcaggaaggt
     9301 atgagccccg aacgcgcttg gtcgccgtgg attgcggagc tggatatcta tcgtcaggat
     9361 tgtgcgcatg tggatattat ctctccaggg gcgtttgtaa aaattgggcc gattattcgc
     9421 gcaacgctaa acaggtaaat taatattatc tataaaccca ta
//
LOCUS       DADLSA010000146         9430 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.147, whole genome shotgun sequence.
ACCESSION   DADLSA010000146 DADLSA010000000
VERSION     DADLSA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.147"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(233..991)
                     /locus_tag="JN691_003382"
     CDS             complement(233..991)
                     /locus_tag="JN691_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY7244736.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELDEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(999..2102)
                     /locus_tag="JN691_003383"
     CDS             complement(999..2102)
                     /locus_tag="JN691_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY7244737.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2112..3293)
                     /locus_tag="JN691_003384"
     CDS             complement(2112..3293)
                     /locus_tag="JN691_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY7244738.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3361..4386)
                     /locus_tag="JN691_003385"
     CDS             complement(3361..4386)
                     /locus_tag="JN691_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7244739.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4817..5038)
                     /locus_tag="JN691_003386"
     CDS             complement(4817..5038)
                     /locus_tag="JN691_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244740.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5291..6675)
                     /locus_tag="JN691_003387"
                     /pseudo
     CDS             complement(5291..6675)
                     /locus_tag="JN691_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018912.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6678..8300)
                     /locus_tag="JN691_003388"
     CDS             complement(6678..8300)
                     /locus_tag="JN691_003388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAY7244741.1"
                     /translation="MPVALYPTIAPPAVSVSANYPGADAQTVQDTVTQVIEQNMNGID
                     NLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQQQGISVEK
                     SSSSYLMVAGFVSDNPDTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQYAMRIWLD
                     ADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRLKNPEEFG
                     KVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALDTAKA
                     IKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQNMR
                     ATLIPTIAVPVVLLGTFAILATFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMM
                     EDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA
                     LSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGST
                     GRYLLIYALIVAGMVVLFLRLPSYLKRIRVSF"
     gene            complement(8308..9375)
                     /gene="acrE"
                     /locus_tag="JN691_003389"
                     /pseudo
     CDS             complement(8308..9375)
                     /gene="acrE"
                     /locus_tag="JN691_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
BASE COUNT         2514 a         2450 c         2253 g         2213 t
ORIGIN      
        1 catcaaataa aacaaaaggc ccagtcttcc gactgagcct tttgttttat ctgttgtttg
       61 tcggtgaaca ctctcccgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
      121 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccagaca tcaaatcaag
      181 caaaaaggcc atccgaaagg atggcctttt tgcttttcga actaacattc aattaatgga
      241 ttacctgcga taaaaatgcc ctcgtacgct ctgatttagg atgcgcaaaa aattcatcag
      301 gtgtagcttg ttccactatc tccccacgat ccataaaaat tacccggtca gcgacggtac
      361 gtgcaaaccc catctcatgt gttacacaca acattgtcat acccgactgc gccagcccaa
      421 tcatcgtatc cagcacctct ttcaccatct ccggatcgag cgccgacgtt ggttcatcaa
      481 acaacataat tttcggcttc atacacagcg aacgcgcaat ggcaacgcgt tgctgctgac
      541 cacctgaaat ctgtccggga aacttatgcg catgttcggc aattctcacc cgctcaaggt
      601 aatgcatcgc cagagcttca gcctctttct taggcatctt gcgtacccaa atcggtgcca
      661 gggtacagtt ctgtaaaacg gtcagatgag ggaagagatt gaaatgctga aataccattc
      721 ccacttcctg cctgacgcgc tcaatattgc ggatatcttc atcaagttcg atgccatcta
      781 cgacaatccg tccctgttga tgttcttcca gatgattaat acaacgaatg gttgtcgatt
      841 taccggaacc agaagggcca cacagaacga tccgttctcc cggttgcacg gttaaattta
      901 tatttttcaa aacatggaat tgtccatacc atttatggac gttttccagc gtaatcatcg
      961 cgttagcagg ttgcagtaaa atttggctca tagtgtcctc aatgcggtgt acgcccggtg
     1021 ttaaaacgtt tttccagatg ttggctataa cgtgacatgc tgaaacaaaa gatccagtag
     1081 atcagcgcgg cgaaaacata cccttccgtc gacataccca accaggcggg atcaacggtt
     1141 gcctgctgaa cgctactgaa aagatcgaac aagccgatga tgatcaccag gctggtatct
     1201 ttgaagagtg cgatgatggt atttaccagc ccaggaatta ccagcttcaa cgcctgtggc
     1261 agaataacca gcccctgagt tttccagtaa cccaacgcca gcgactctgc cgcttcatat
     1321 tgccctttag gcagcgcctg taatccacct cgcacgactt ccgcaacata tgctgactga
     1381 aacaggatca cgccaaccag cgcgcggatc agtttgtcga tactggtgcc ttctgccata
     1441 aacaacggca gcatgaccga agacataaac aatacggtaa tcagcggtac gccgcgccag
     1501 aactcgataa aaatgaccga taagatacgc acaatcggca tatgggagcg gcgacctaat
     1561 gccagtaaga tcccccacgg tagcgccccc gcaatcccaa cggatgcaat aattaaagtt
     1621 agcgtcagcc cgccccattg ccgggtttca acccgctcaa gaccaaaaaa accgccatac
     1681 atcagccacc agacaatcag tgggtaaatc accgcccagg ccgcaatata gcgaccgcga
     1741 tgcgggagta ttttccagaa cattggtgcg atcgatacaa gcccaatcag taatgcgagg
     1801 ttaattcgcc agcgttggtc gtgtgggtaa agcccataca taaactgacc aaatcgttcg
     1861 tgaatgaaga cccaacaggc accggctttt gtgcagtcag cacgcgttga gccaacccaa
     1921 ttagcctgca aaaatgccca gttcagcaac ggtgggatca gttcccacat caaccatatg
     1981 cagccaatag tcagcaggct attgctccag ctggagaaca gattttttcg cacccacacc
     2041 atcgcgcggc ttgagttatg gctcgccggg cgcgagggct gagacagcaa tacttttgtc
     2101 atcatgactc cttagcgttc aacgatcgcg atgcggcggt tatagatatt catcagcagc
     2161 gagatagtca ggctgataat cagatagacc gacatggtca tggctatcgt ctcgatggct
     2221 tgccccgtct ggttcagcac ggtgccagca aacagcgaaa ccatatcggg atagccaata
     2281 gcggcggcaa gagaggagtt tttgacgatg ttgagatact ggctggttaa cggcggaata
     2341 attactcgca gtgcctgggg aataatgacc tggcgtagcg taaccgggtt gggtaacccc
     2401 agtgaccgag ccgcttcatg ttgaccataa ggcactgcct ggatcccagc gcggataatc
     2461 tcggcgataa atgcagaggt ataaaccgaa agtgccagcg ttaaggctgc cagttcagga
     2521 attaaaacca ttccgccgcg gaagttaaag ccttgtaggg ctgggacatc ccagtgtagt
     2581 gctgcgccaa aaagccattg cgccagtaaa ggcaaaccaa tgatcaacac tgcggcgatc
     2641 ggccaggagc ggcgcagttg tccggtcttt atctggtatg ttttattaaa gcggaatagc
     2701 ccaacagaaa ggactatagc cataacaaca gccaggataa acgcaataaa tccgtctccc
     2761 agctgcggtg acggaatata aagcccacga ttgctcaaaa aggccagatc aaacgcgctc
     2821 acagcttggc gtggtccggg caaattgcgc aacacggcaa agtaccagaa gaagatttgc
     2881 agcagcgggg gaatattacg gaagatctca atataaattg tggaaagctt tcgtagcagc
     2941 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     3001 cacaatgcag aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg
     3061 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     3121 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt gtgaaataac
     3181 caaccaacaa tgccgacaac agcaacaacg gcaaggatct ggaataacca ggcacgaacc
     3241 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3301 tcggaaagca taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac
     3361 ttaacgcacg ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3421 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3481 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3541 gccttcttta cccaacagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3601 gacgttctgg gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg
     3661 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3721 gataacctcc ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcaaggcata
     3781 cagttgcgac tgatccgaag ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     3841 ttcgtcagag cgatcgaaag tcactggtgt gtacttcata ttgtttgcct tgaaatagtc
     3901 ggcgacgttg agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc
     3961 tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt
     4021 gacgccggta aatgccattc ccatcccggc atcgcgagat gaagtccagg tcgtattacg
     4081 ggagagcaaa tccacttccc ctgactgtaa agcggtgaag cgttcttttg ctgtgagtgg
     4141 ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaacatc
     4201 aacatcaata cctgaaaact taccgtctgc atcggcataa gagaacctag gtaatccatc
     4261 actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg
     4321 cgcctgattt gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt
     4381 tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt
     4441 tacctgtctg tggccatcct cttataagca aaagtagtgc cagcttttgc gcacgatttc
     4501 gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtgcg
     4561 aaaatgaaac atgcgccaca ttttggcgca aaatatttac gcgtgtttta ctaagcagaa
     4621 taatgattgt tagcgtgagt atatttctgg tgggaagtgt gaaagttaaa ttgatgcgtt
     4681 atttcgaaag cagcgaaata acgcgatata acatgctaaa tcagcagaga attagccagc
     4741 agaatatagc aatattttat cttatgtaaa gatactgtat cgttaaacag agcgcgaaac
     4801 tccgcgccct gttcagttat ttcgacagag cgatgatccc catcgtcagg ccagcaacag
     4861 ccgctgcccc gccagcaata gcaccagccg tttcccagtt atcctgagtc gtgcctttca
     4921 tacaggtatt ggtgtgaacc agacgaccct gatcgtcata aaccggaacg cagggcgaat
     4981 cgtgagcaca gcccgccagc aatgcggtga gcaatgccac aggaattaat cttttcatgg
     5041 tgttacctca gtaataaacg ctaacgctta ataagttaac tctcttatta acgagctgaa
     5101 tgccatttta tcaccggagg ttataaatct tgcggaatga ataatgacaa attatgtgaa
     5161 ttgtaaaaat agtgaaggga gcgggaacta tagaaagctt ttaaagagtg agcagaagaa
     5221 tatcttatta atttttatgg gtttcactgg aaataataaa ggcacccgaa agcgccttta
     5281 tgtttctgat ttatccttta aagcaacggc gaatcaccac aaagaacacc ggtacgaaga
     5341 agattgccag caacgttgca gagaccattc ctcccattac cccgataccc acagcgttct
     5401 gcgcgccact gccggcaccg ttactgatag ctagcggtaa tacgccgaga ataaaggcga
     5461 gagaggtcat caggatagga cgcagacgca tacgtactgc catcagtgtc gcttcaacaa
     5521 cacctttacc ctctttctcc atgagatctt tagcgaactc aacaatcaaa atagcgtttt
     5581 tggccgacaa gccaattgtc gttagcaagc ccaccataaa gtagacgtca tttttttgat
     5641 taaagagtgt cgccgccagc agcacgccga caatccctaa cggcacgacc aacataaccg
     5701 agacaggaat tgaccagctt tcatagagtg cagcaaggca caggaaaaca accacaaagg
     5761 aaattgctac cagagcggga gcctggtttc ccgataagcg ttcctgatac gacatacccg
     5821 tccagtcata accaatgccc gcaggtaatt ttgacgcaag gttttccatc aacgccatgg
     5881 catcgccgga actggttcct ggcgcggctt ccccctgaat ctccattgac ggcagaccgt
     5941 tgtagcgttc cagtcgcgga gagccataca cccaatgtga agtggtaaag gcagagaatg
     6001 gcaccatttc gccgttggcg ctgcggacat aaagtttatc gacatcttct ggcagcatac
     6061 ggaatttggc atccgcctga acatacacct ttttcacgcg accacggtcg atgaagtcgt
     6121 taacgtaagt cccacccaac gccgttgaaa tggtctgatt gatgtcagaa agtgaaacac
     6181 ctaatgccgc gccttttcct ggtcaacttc cagtttaaac tgcgcggtgt cttccaggcc
     6241 attaggacgc acgctgacta agctggcagg atgttgcgcc gccataccaa gcaactggtt
     6301 acgggcctgg gttagggcat cgtgacccag cccagcctga tcaattaact caaagtcgaa
     6361 acccgttgcc gtgcccagtt caacaatggc tggcatattg aatggaatga caaaaccgtc
     6421 gcggatcttg cccaattcca ttttggcacg atggattacc gcttccgcac tgttttcatc
     6481 accattacgc tcttcccacg gtttcagact gacgaaggcc ataccggcgt tttgtgcctg
     6541 gccgctgaag ctaaagccgt taaccgtaaa gacactttca acgttcgctt tctcgttctt
     6601 cagatagtaa tccgtcactt gatccaacac tttttgcgtc cgctcttgcg tcgcgccagc
     6661 gggtaactga atcatggtca gaaagacacc ctgatcctct tcaggtaaga aggaagacgt
     6721 aaaaacaaca ccaccattcc tgcaacaatc agcgcataga tcagtaaata tcgtcctgtg
     6781 gaaccgagga ttttgccgac gctgttggtg tagtggttaa cgctatgatc gaaggtggta
     6841 ttaaaccagc cgaagaaacc gcccttattt tcgtgatgct cagcagagac gggtttaagc
     6901 agcgttgcac ataacgcagg ggtaagaatc aatgccacca gaacagaaag tgccattgcc
     6961 gaaacgatgg tgatagagaa ctggcgataa attgccccag tagaaccgcc gaagaatgcc
     7021 atcggaataa ataccgctga cagcaccatc gcgataccca ccagtgcgcc ctgaatttgc
     7081 gacatcgatt tttccgtcgc ttcttttggc gggagcttat cctccatcat cacgcgctcg
     7141 acgttctcca ccaccactat cgcatcatcg acgagcagcc cgatggcaag caccatcccg
     7201 aacatcgtta gtgtgttgat ggagtaacca aaagtggcga ggatggcaaa cgtccctaac
     7261 aacaccacgg gtaccgcaat ggtggggatc agcgttgctc gcatattctg caagaacaga
     7321 tacatcacca ggaacaccag cataatggct tcgaacagcg tttttaccac ttcgtgaata
     7381 gaaagctgga cgaatggcgt ggtgtcataa gggtagagaa ccttcattcc ctgcgggaag
     7441 aatggctgta attccgccag ttttgcctta atggctttcg cggtatcgag agcattcgcg
     7501 ccggttgcca gcttaatccc caggcccgcc gccggttttc cgttgatacg agcgataacg
     7561 ttatagtttt caccgccaag ttcaacccgt gcgacatctt tcaggcgtac caccgagccg
     7621 tcactgttta cgcgcagggt cactttgccg aattcttccg gattttttaa ccgcgtttga
     7681 gcaataatcg aggcattcaa ctgttgccct ggtaacgctg gcgttccgcc caactgtccg
     7741 gcagcgatct gatcgttctg taccttcaac tggttaatca catcaaccgg tgtcagttta
     7801 tatttgttta gcagatcggc gtccagccag atacgcatcg catactgtgc gccgaaaagc
     7861 tgtacgtcac cgacgccatt cagacggcta agcgtatctt taacgttaga ggccacatag
     7921 tccgagatat cgtcctgtgt ggtgtctggg ttatcagaga caaagcccgc caccatcaaa
     7981 tagctgctac tggacttttc aacactgatc ccctgctgct gaacctcctg cggcagcaac
     8041 ggcgtggcga gctggagttt gttctgcacc tgcacttgcg cgatatcagg atcggtcccg
     8101 gactggaagg taagggtaat tgtcacgcta ccggcggaat cgctggtgga ggacatatac
     8161 atcaggttat cgataccatt catattctgt tcgataacct gcgtcaccgt atcctgcacg
     8221 gtctgcgcat cagcacctgg atagtttgcc gaaacagaaa ccgcaggcgg tgcaatggtt
     8281 ggatacagag cgacgggcaa ttgtaggatc gccagatttc agaccttcgc taatcaacca
     8341 tttatcgcca atcgcctgac tggcaacgac agggcgcgct tccacctgac ttttatcgtt
     8401 aacaatcagc acggttgcat caccacgcgg tgtgcggcta acgccttgtt gcgggataag
     8461 aatggcgtca ggttggacgc cttcatcaat ccgtgcacgc acaaacatac ccggcaaaag
     8521 cgtatgttgc gggttaggga agacagcacg tagggttatg gagccggtgc tttcatcaac
     8581 ggtcatatca gagaattgca gcgtaccttt caggggatag gtttgaccgt tttccatgac
     8641 caactctacg ttgctggtgg cgttttcctt atgcaaattt ccttgctcta cggattgctt
     8701 cagcctcata aaatcgttgc tggattgggt cacatcaacg tagataggat agagctgctg
     8761 gacagtcgcc agttcagtcg tttgcccatt agtgacaaga gcgccttcgg tcacggtcga
     8821 tttgccgata cgtccgctaa ttggcgcagt cactttggta taagcgagat tgatgcgagc
     8881 gctttcgact gtggcttttg cggcaatcac ggcggcatcg gcctggcgag catcagcaat
     8941 ggcctggtcg tactcctgct ggctgatgta tttcgtaccc acgagcggaa cgtaacgttt
     9001 taccgtcaaa tgcgcgatgg cggcggcggc ttcacttttc gccagttcgc ctttcgcgct
     9061 gtcataattt gcctgatagg tcgcgggatc gatctggtat agggactggc ctgcttgtac
     9121 atcgctgcct tcagtgaaat tacgattcag tacgatcccg ctaacctgtg ggcgaacttc
     9181 ggctatacgg taagcattgg tgcggcctgg taattcagtc ttaacttcta acggggccgt
     9241 tttcacaata tgaacggtaa cctgcggttc accgacgtga gctttctctt ctcccttatc
     9301 gttacaaccg gcgattaaag ccgcggagat cagaataaag gagggcagga ggaaaaacct
     9361 ggcatgtttc gtcattacta ttcctcaaaa aaccaaaagc gcgttattta cccagaaagg
     9421 caacgcgtta
//
LOCUS       DADLSA010000147         9298 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.148, whole genome shotgun sequence.
ACCESSION   DADLSA010000147 DADLSA010000000
VERSION     DADLSA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.148"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(71..259)
                     /locus_tag="JN691_003390"
     CDS             complement(71..259)
                     /locus_tag="JN691_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HAY7244742.1"
                     /translation="MYKITATIEKEGGSPTSWTRYSKTKLTKSECEKMLSGEKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            complement(310..654)
                     /locus_tag="JN691_003391"
     CDS             complement(310..654)
                     /locus_tag="JN691_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244743.1"
                     /translation="MDTSRDNSELFANDGTPTWLKEKRAKEIALDEIIELLNKHHGGT
                     SNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIELEGVEYAFPDPQTQNE
                     TQQAGYDENIPV"
     gene            complement(794..2215)
                     /locus_tag="JN691_003392"
                     /pseudo
     CDS             complement(794..2215)
                     /locus_tag="JN691_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
     gene            complement(2239..2535)
                     /locus_tag="JN691_003393"
     CDS             complement(2239..2535)
                     /locus_tag="JN691_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HAY7244744.1"
                     /translation="MSIIECEIELDVINDSLAEFKCGNTLHSFIQISQKENVTVIIDD
                     GYVLGTFDCCVCAIKEIALLTARIEEADKKYGISYQRLKELSHIASSVRFFYAI"
     gene            complement(2610..2897)
                     /locus_tag="JN691_003394"
     CDS             complement(2610..2897)
                     /locus_tag="JN691_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244745.1"
                     /translation="MDMNPMLDYCFTPALRQTISHTGIKKWLWRIRKILQQKGDPDLI
                     QCDYHLCNKQFLPEKAFTGELIYMHGNMIARKKRKYCGAACAEKDKMAHEL"
     gene            complement(2908..3747)
                     /locus_tag="JN691_003395"
     CDS             complement(2908..3747)
                     /locus_tag="JN691_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7244746.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGISTPKVTRAHNRAEAVFLC
                     VMHSHIQIMVRRAGQPQGWPGSRVTGSANPVRLTTHEISTSAGELINLSLEDVIMATT
                     LSHPDVTIENGRAVTTSISIAEFFGKQHHHVVQKIESLECSELFLTSNFSRVKFEHRG
                     NSYNAYQITKNGFVFLVMGFTGKKAATFKEAYIAEFDRMEAELRQNNTPPADKMIPGD
                     GRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQLL
                     SLK"
     gene            complement(4169..4321)
                     /locus_tag="JN691_003396"
     CDS             complement(4169..4321)
                     /locus_tag="JN691_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HAY7244747.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LAKNTS"
     gene            complement(4514..4921)
                     /locus_tag="JN691_003397"
     CDS             complement(4514..4921)
                     /locus_tag="JN691_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7244748.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNDLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAGVLQCSPTWILFGDEDKTPDPPVALNSALDLSEDELEMLRLYRALPKS
                     EQQAQISELRARVENFNRLFTELLEARKRNKHQ"
     gene            4999..5226
                     /locus_tag="JN691_003398"
     CDS             4999..5226
                     /locus_tag="JN691_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7244749.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWGELVPEGRAMRLQK
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            5210..5632
                     /locus_tag="JN691_003399"
     CDS             5210..5632
                     /locus_tag="JN691_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244750.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGASDDSVVVH"
     gene            5723..6478
                     /locus_tag="JN691_003400"
     CDS             5723..6478
                     /locus_tag="JN691_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016060759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7244751.1"
                     /translation="MSMKLMAKAMEIKVGNPLRKLVLIKLADNASDQGECWPSYQYIA
                     DQCEISKRSVINHIAALCESGLVKKITRKGEKGNTSNIYLLHLDGAENLPGNSANNSL
                     HCASDASGSAGVAPVASAGVAPRTSHSFEPVNEPDVGGSADADPQVSHRAKNKIDWQR
                     VLDSYHEILPEMPSVKILTDTRKKNLRTFWQKFGFDQQRWESYLRYVAENCRWMLEDR
                     PNGSGGFWKRKNLDYLITERCYVAVKEARANDR"
     gene            6468..7871
                     /locus_tag="JN691_003401"
     CDS             6468..7871
                     /locus_tag="JN691_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7244752.1"
                     /translation="MTDNTFPVPYRVDAEQAVLGCLMVNTDTERAGLVYSMLKPESFY
                     VAAHRVIFREIRGLFRAGKPTDLLSLANVIEAKKLDAETGGFAYLAEISRSATLSAMV
                     HYAGIVREKSILRYAVEKLHACIGIMNQPTDTSATERLGAVQQVIGTVAEYARTGKTG
                     GLRPVSDVVNDWIDDLERRFSSPENAAGLTLGISSLDRLMAPKHVLRGSLVVIGARPK
                     MGKSASLNRIVTHFALNHRLPTLVFTLEMTDRSLVERMVAQEARVNSEIFYVGASDDS
                     DMARAMAKAAELAESNLMIDSTPGVTLAHVIAECRKVKRQRGVVGLVAIDYLTLMKAE
                     AAERRDIAFGDITTGLKNLAKELDCVVLLLTQLNRKLEDRADKRPTPSDSRDTGQIEQ
                     DCDVWIGLYRDAVYNRNADSQLMELILRLNREGATGTAYALMDNGSVTDVSAEDAARR
                     LRLGGPARKPYAQTEDF"
     gene            8038..8799
                     /locus_tag="JN691_003402"
     CDS             8038..8799
                     /locus_tag="JN691_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HAY7244753.1"
                     /translation="METVFDALKAMGKATSTELAARLDISREEVLNELWELKKAGFVD
                     KSAYTRRVADNNVQQEQPAQAELPEETTTATVAKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQGGKFRYCIPGDNLPAEPKAASVAETDGKAFPQ
                     PAGVALPVQEAATQEDIKTETVADIVQSLPLFTETRADDLVLPSLHMANRELRRAKSH
                     VQKWECVCAALRELNKHRDIVRQITDISRRVASEK"
     gene            8815..9207
                     /locus_tag="JN691_003403"
     CDS             8815..9207
                     /locus_tag="JN691_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY7244754.1"
                     /translation="MAKVFTPEEREEVKARIVELVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSDCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYDRRQN
                     IICRECRKSEVMQRVLAFYQGNFQEVMA"
     gene            9204..>9298
                     /locus_tag="JN691_003404"
     CDS             9204..>9298
                     /locus_tag="JN691_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HAY7244755.1"
                     /translation="MRVRVYIAGPMTGYKNFNREAFHNAEEELKR"
BASE COUNT         2366 a         2076 c         2403 g         2453 t
ORIGIN      
        1 acagaatact ggcgggtgtg gttgattttt aataatcagc tatgaagttc taatctggat
       61 ataatgaaat tcacgaggac tgaagtttct cgcaattaaa atttatcagt tttactttct
      121 gctctctgga aacgcctgct tctttttccc ctgagagcat tttttcgcat tctgatttcg
      181 ttagttttgt ctttgagtat cttgtccagc tggtaggaga tccaccttcc ttttcaatag
      241 tggctgtaat tttatacatg aacacctcca ttaatatttt caaaaaccgt gtccaaaatg
      301 cacttggttt tacaccggaa tattctcatc gtatcctgct tgttgcgttt cgttttgtgt
      361 ttgtggatcc gggaatgcat attcaacgcc ttccagttca atccatagtg cattacgacc
      421 agctttgatt gtcggccagt ccataccttt gattctttcc catgagcggg aggtgaatgt
      481 tttttccagc aaatcagctt ttgcgcgttt ggcgtcgtta ctggtgcccc catggtgctt
      541 gttgagcaac tcgataattt catcaagtgc gatttctttt gcacgctttt cttttagcca
      601 tgtcggtgta ccgtcgttgg caaataattc actgttatcc cgtgaggtat cgatgccgag
      661 gtgttcacct cccaggttaa gaaactcgat atgaggaaga aagtttttaa attttgactt
      721 catttgagga gatatgatga ccgggaatcc atttcgaatc attcgggtcg ctaatccctt
      781 caacaaattc tccgcgagag gcagccagta atttgtctgc atcgacagga tttttgggcg
      841 gaatgttttt ccgggcttca tggagttctg tccgcagttt ctgatatttc gcatcaacag
      901 catttacctg tgactgagca tccagcggct gcgtgtcctg atgatgttca gttgcatccg
      961 gttccactgt ttcagccgtt gcctgttcat ctgccattgc gcaagatggt tgcggttttt
     1021 cttcatcatc ctgttttcct tcttctgtta cacgttgcgg catcggggca gaggagcgac
     1081 cgcaggcaat atccacgatt tccggatcag ggttggcatg atcggtttca gtcagtacct
     1141 tgttcagata ttcagtgacg tgcgcgggga tgacctcgat accaatcggt gcatctttca
     1201 ctgacgcaac cacgatggcg cgggaataat ccatcccgcc aggcatggtg atgaatttgt
     1261 cgcggaaaac agaaaagggc ggtttatttt cagcgataat ttcctcgaca cgtttagcgt
     1321 gtgccggatg aaggttataa atgtccacgt ccattgaacg agccagtacg ccagtggcta
     1381 cgtcgcgcgc cagtgacgtc agatcgtgaa cgaaaccttc gccgcgatcg gtgaggttcc
     1441 cgccgccagc attagcaccg gaagccgtgc gagtgatacg cgaaacacga tttcctttca
     1501 tccactcttt tgtcagcaga ccgcgatcgg tgtagttggc atccagatat gcttcgaaaa
     1561 aagcagtcat cagtcccaga tttgaattgc caggattagg gaaaactttg tcagtatcac
     1621 gcacgagttt gtggaggtcg cgaatctcca gcgggtcgag cagactggtt ttatgcgaaa
     1681 cagccagagc agtaacagcc ggtagttctt catcccgagc aatgtgtaat gcctggagtt
     1741 cgtcgcgtga aacgtgcgtt accggttttt cgctgccgtg ttgcgcaagc caacgaatgg
     1801 gcagttcctg accggaaact ggcaggagca tgttctcctc aatttctgtc atgtcttcgc
     1861 cgttgacgtt ggtattgtca gtgctggctg gtttgtcctg aacagaggga gaagatgcga
     1921 taaataccat tgtgatgcca tcttccccgc ctttttcgta acggttgcag aattccgtat
     1981 caaacacgcc ttctggtggg aggtcatcaa caacgggcaa attgacgcga acaggttttt
     2041 taaagtcatc ttcatcgtag ccagcatcgt caatcgcaac agcaccacgg gagatggcaa
     2101 tggataattt tttcgcttca gcccagtaaa aaccgccttt aataccgagg cgttttctta
     2161 ctttgtcatt ttttgcttcg taatacagtg ggtaaacttg tttatcggtg ctcattgttt
     2221 tttaacctca actcatattc agattgcata aaagaaacga actgatgatg cgatatgaga
     2281 gagttctttc agtcgttgat aactgatacc gtatttcttg tcagcctctt caatgcgagc
     2341 tgtgagtaat gcaatttctt taatggcaca tacacaacaa tcaaatgttc ccagaacgta
     2401 accgtcatca ataattaccg tgacgttttc tttttgggaa atctgaatga aactatgtaa
     2461 tgtattccca catttaaatt cggcgagaga atcgttaata acatcaagtt caatttcaca
     2521 ttcaataatg ctcattgctg tttccttttt taaggttgag tgaatccctg ccattgctgg
     2581 cataaattca gtttcgaata ttcagttaat taaagttcgt gtgccatctt gtctttttcg
     2641 gcacaagctg caccgcaata ttttcgtttc tttctggcaa tcatgtttcc atgcatataa
     2701 ataagctcgc cagtaaaagc tttttcaggt aaaaactgtt tattgcagag atggtaatca
     2761 cattgtatta aatccgggtc acctttttgc tggagaattt ttctgattct ccacagccat
     2821 tttttaatcc cggtatgact gatggtctgc ctgagggcag gtgtaaaaca ataatcaagc
     2881 atggggttca tatccatagc tccattttta ttttaaggaa agcaactgct ctacggtcat
     2941 gtttttaatt gcgccccggt ttacaagagt ccagccctgt ttttccagat aaaaccggaa
     3001 agtctccagg gtacagacca gtgcgccatc aggaacggtt tcggtgaatt cgacattgcc
     3061 gaatttgtcg aagtgaacaa ccagagtgcg accatcaccc ggaatcatct tgtcagcagg
     3121 tggggtgtta ttctggcgca gttcggcctc catgcggtcg aactcagcaa tgtaggcttc
     3181 cttgaatgtg gcggcttttt tgcccgtgaa gcccatcaca aggaaaacga agccgttttt
     3241 ggtgatttgg taggcgttat aagaattgcc gcggtgttcg aatttaaccc gcgaaaagtt
     3301 gctggttaaa aatagttcag aacattcgag ggattctatt ttttgaacaa catggtgatg
     3361 ctgcttgcca aaaaactcag caatcgaaat agaagtagtg acggcgcgac cattttcgat
     3421 ggttacgtca gggtgagaaa gggtagtagc cataatgaca tcctcaagtg ataagttaat
     3481 taactcacca gcagaggtgc taatctcatg ggtggtgaga cgcacagggt tagcactacc
     3541 ggtcactcga gaacccggcc agccttgcgg ctgccccgca cgcctcacca taatttgaat
     3601 gtggctgtgc attacgcata aaaaaaccgc ttcagcgcgg ttatgcgctc gagtaacttt
     3661 cggggtgcta atcccggcac ccgttttatg aggtgcaggt gcactataat tccacccgtt
     3721 ctggttttca atagctacat tcaacatttt ctctttcctt tcatcaccga agtgaatttt
     3781 gtgatgcggt gcctggtgca tccaggtgac gttaaccagt taacaattac agtcggcttt
     3841 cccacccaaa acaataagga ctaacatgac ttttaactgt gccgcgtgcg cttagccgca
     3901 ttcaccgcat cacaaaattc actttaaaaa gggcggacat cagccagcaa ttaaaccgat
     3961 gccgccaact ggtacttcac acagcaatgt cgttatttac aaccggaagc gcactcccac
     4021 catttaaatt tcacagacaa gaccgactct ttatgaatac cggaaatgcg ccttcgtgtt
     4081 gtgcccggtt ttatttcacc acctccgggc tttggtggcc tcggctatac ctctacagcg
     4141 agaatattga attaatccaa taaatggatt agctggtatt tttggcaagc cagcgacgtg
     4201 cgccagcttc ggttttaaac gttttgcttt tggtatacgt catggcggtg aatgtgccgt
     4261 cctgattggg aaacacgcca cacactaggg attcgttgtt gccaagatcg atagtatcca
     4321 tgctgacctc atttcccctt aacgccgggg tggcggaact gtttactgag agcaccgtgc
     4381 ggtgtcttga tagatgttaa tttagttttc tcatgataat tgatcaagta tttttgatga
     4441 gaaatctcaa tatttaatgc aaaataaagc caatacattg aaatgtaagg ctttaaaatt
     4501 tgtgaagggg gggttattga tgtttgttac gtttgcgagc ttctagtagc tcggtgaata
     4561 ggcgattaaa attctcaacg cgggcacgga gttcgctgat ttgtgcttgc tgctctgatt
     4621 ttggaagtgc gcgatacaat cgcaacatct ccaactcatc ttccgataag tctaaggcgc
     4681 tgttgagtgc aactggtgga tctggtgttt tatcctcgtc accaaacagt atccaagttg
     4741 gtgaacattg caatacccca gccaggcgat gcaaattttg cccgcgcggg gctgtatggt
     4801 cgctttccca tagtgaaatt gatgagccag atacgccagc agctttgctt aaatcgtttt
     4861 gacttaaacc aacctgtttg cgtctttctc taattcgttg acctaaagtt ttctcgttca
     4921 tatttagata tcttaataac ccttgacttg agattccttg agtgattact tttgagaaaa
     4981 ctcaactttg gaggggtgat gtttaaatca gacgtaatta atttttatgg gacgaaagcc
     5041 aaagtagcga aagctgctgg tgttgatcca tctgctgttt ctcaatgggg tgaactggtt
     5101 cccgaaggtc gcgcgatgcg cctacaaaaa gcatctggcg gggagcttca gtacgacccc
     5161 aaagtttatg acgaatatcg taaggcaaaa cgacctggga aggtgattca tgaaaatcag
     5221 gcatgagcac atcgaatcag tgctgttagc cctggcagcc gaaaaagggc aggcgtgggt
     5281 cgctaacgca attactgaag aatatctgcg ccaggggggc ggcgaattgc ccctggtacc
     5341 aggcaaggac tggaacaatc agcagaatat ctatcaccgt tggttgaaag gtgaaacgaa
     5401 agcgcaaagg gaaaaaattc agaaactgat ccctgcggtt ctggcaattc ttccgcgcga
     5461 gctgcgtcac cgactctgta tcttcgatac cctggaacgc cgtgcattac tggcggcgca
     5521 ggaagcgttg agtacggcaa ttgatgcgca tgatgacgca gtccaggccg tttaccgtaa
     5581 agcgcatttc agcggtggtg gggcgtccga cgattctgtc gttgtgcatt gatgtttatg
     5641 ccgaacctcc tctggttctg ttgattgggg aatcacagat tatatccgga ggaaggttcg
     5701 gcaccagacg aggtttctat atatgagcat gaaactaatg gcaaaagcaa tggaaattaa
     5761 agtgggtaat ccattgcgta agctggttct gattaagctg gctgataatg ccagcgatca
     5821 gggcgagtgc tggcccagct accagtatat cgctgaccag tgcgagatta gcaagcgttc
     5881 tgtgattaat catattgcgg cgctttgtga gtccgggctg gtaaaaaaaa tcacccggaa
     5941 aggtgaaaaa ggtaatacaa gtaatatcta tctccttcat ctggatggtg cagaaaattt
     6001 accggggaat agtgcaaata attcgctaca ttgtgcatca gatgcatcgg gtagcgcagg
     6061 agttgcacct gttgccagtg caggagttgc acccagaacc agtcactctt ttgaaccagt
     6121 caatgaacct gatgttggtg gatctgctga cgcagatcca caggtcagtc atcgcgcaaa
     6181 aaacaaaatc gactggcaga gggttctgga cagctatcac gaaatcctgc ctgaaatgcc
     6241 atcagtgaaa attctgacgg ataccaggaa aaaaaatctg cggacgttct ggcagaaatt
     6301 cggttttgac cagcagcgat gggagtccta tctgcgatac gttgctgaga actgtcgctg
     6361 gatgctggaa gatcgtccca atggcagcgg tgggttctgg aagcgtaaaa acctggatta
     6421 cctgatcacc gaacggtgct atgtcgctgt caaagaggcg cgggcaaatg acagataaca
     6481 cctttccggt gccataccgg gtcgatgccg aacaggcagt gctggggtgc ctgatggtaa
     6541 acaccgacac tgagcgtgcg gggctggtgt attcgatgct gaaaccggag tcgttttacg
     6601 ttgcggcgca cagggttatt ttccgtgaaa ttcgcggact tttccgtgcc ggaaagccta
     6661 cggacctgct cagccttgcg aacgtgatcg aggcgaaaaa actggatgct gagactggtg
     6721 gctttgcgta ccttgctgaa atcagcagga gtgccacgct atcggcgatg gttcactacg
     6781 ccgggattgt gcgggaaaaa tccatcctgc ggtacgcggt ggaaaaatta catgcgtgca
     6841 tcgggatcat gaatcagccg acagacacga gtgcgacaga acgacttggc gcagttcaac
     6901 aggtcattgg cacggtggca gagtatgcca gaacaggaaa aacaggcggt ctgcgcccgg
     6961 taagtgatgt ggttaacgac tggattgatg atcttgagcg tcgcttttcc agcccggaga
     7021 acgcagcggg tctgacgctg gggattagct ctctggatcg gcttatggcc cccaaacatg
     7081 tgctacgtgg ttcgctggta gtgattggtg caaggccgaa aatgggtaaa agcgcctcgc
     7141 tgaacaggat tgtgacgcat tttgctctga atcatcgtct gcccacactg gtattcaccc
     7201 tggaaatgac agatcgcagc ctggttgagc gcatggttgc gcaggaggcg agggtaaatt
     7261 ctgaaatttt ttatgtcggt gccagtgatg actcggatat ggcgcgggcg atggcgaaag
     7321 ccgcagaact ggcagaatca aacctgatga ttgacagcac accaggggta acgctggccc
     7381 atgtgatcgc tgaatgccgg aaggttaagc gtcagcgtgg tgttgttggc cttgtggcga
     7441 tcgattacct gacgctgatg aaggcggaag ccgcggagcg tcgtgatatt gctttcgggg
     7501 atatcacaac gggcttaaaa aatctcgcta aagagctgga ctgcgtggtt ttacttctga
     7561 cgcaacttaa ccgaaaactc gaggacaggg cagataaacg ccccacacca agcgacagtc
     7621 gcgacaccgg gcagattgaa caggattgcg atgtgtggat tgggttgtac cgggatgctg
     7681 tctacaaccg taacgcagac tctcagctta tggagctgat tctccgtctg aacagggagg
     7741 gagcaacagg aacagcatac gccctgatgg ataacggttc agtgactgat gtcagcgcag
     7801 aggatgctgc cagacgtctg agacttggtg gtccggcacg taaaccctac gcacagacgg
     7861 aggatttctg atgcagtcac tttgtgatgt tctggaaaaa acgctgcttc agttcgggcg
     7921 gggggaaacg gcggagcaga aaacacgaaa ccagaagaat ctggttcatc tgagaaaaat
     7981 gattgatgca gccaggctgg ctgaaaagct ggaaaaacaa aaccggaggt aattttaatg
     8041 gaaactgttt ttgacgcact gaaagcaatg ggaaaagcca catccacaga acttgctgcg
     8101 cgacttgata tcagtcgtga agaagtgctg aacgaactat gggaactgaa aaaggctggt
     8161 tttgttgata aaagcgcgta cacccggcgt gtggctgata acaacgttca gcaggaacag
     8221 ccagcgcagg cagaactgcc ggaagaaacc accacagcaa cagtagcgaa aatctcagag
     8281 tgcgatttaa ccgcgacgat tgaacaacgc ggaccacaaa cggctgatga gctggctaca
     8341 ttgtttggta ccacatcacg caaagtggct tcaacgctgg caatggcaat cagcaaaggt
     8401 cgtctgattc gcgtaaatca gggcggtaaa tttcgttact gcataccggg cgataattta
     8461 ccagcagagc cgaaagcagc atcggtagcg gaaactgatg gtaaggcctt tcctcagccc
     8521 gcaggtgttg cattaccagt acaggaggct gcaacacagg aagatattaa aacagaaacg
     8581 gtggcggaca ttgtgcagtc gctgccattg ttcaccgaaa cgcgagcgga tgacctggtt
     8641 ttaccatcgc tgcatatggc aaaccgcgaa ctgcgtcggg cgaaaagtca tgtccagaag
     8701 tgggagtgtg tctgcgccgc gctgcgggag ctgaacaagc accgggatat tgttcgacag
     8761 attactgata tttctcgccg tgttgcatcg gaaaagtgat tgccggaggc acctatggca
     8821 aaagtattta caccagaaga gcgggaagaa gtgaaggcgc gcattgtgga actcgtgcgc
     8881 ctgagcggac gagaaacttt tcgacaactg gcagataaaa cgggtgtcag taagaccgct
     8941 attcgtcgtt tatctggtgc gcttgcggcc agtggtgatg tctggctctc tgattgcggg
     9001 gtatttccat cagagcaggc gtatcgcgta tggcgtaaga caccggagaa ggctgctgac
     9061 ccgacactga ttcgaaagtt acctgacgga gaaatacgcc gctacgacag gcgtcagaac
     9121 ataatctgtc gcgagtgccg gaagagtgag gttatgcagc gagtgctggc gttttatcag
     9181 ggtaattttc aggaggtgat ggcgtgaggg tgagagtcta tatcgccggt ccaatgaccg
     9241 ggtataaaaa tttcaaccgt gaggcgtttc acaatgcgga agaggaactg aaacggga
//
LOCUS       DADLSA010000148         9279 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.149, whole genome shotgun sequence.
ACCESSION   DADLSA010000148 DADLSA010000000
VERSION     DADLSA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.149"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(2..94)
                     /locus_tag="JN691_003405"
     tRNA            complement(2..94)
                     /locus_tag="JN691_003405"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="JN691_003406"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="JN691_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAY7244756.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JN691_003407"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JN691_003407"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAY7244757.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKQEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLGRTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVTEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JN691_003408"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JN691_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAY7244758.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JN691_003409"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JN691_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAY7244759.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JN691_003410"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JN691_003410"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAY7244760.1"
                     /translation="MTDSELMQLSEQVGQTLKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6113..7198)
                     /gene="mltB"
                     /locus_tag="JN691_003411"
     CDS             complement(6113..7198)
                     /gene="mltB"
                     /locus_tag="JN691_003411"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAY7244761.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7454..8017
                     /gene="srlA"
                     /locus_tag="JN691_003412"
                     /gene_synonym="gutA"
     CDS             7454..8017
                     /gene="srlA"
                     /locus_tag="JN691_003412"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAY7244762.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="JN691_003413"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="JN691_003413"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAY7244763.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGIKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..>9279
                     /gene="srlB"
                     /locus_tag="JN691_003414"
                     /gene_synonym="gutB"
     CDS             8984..>9279
                     /gene="srlB"
                     /locus_tag="JN691_003414"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAY7244764.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGT"
BASE COUNT         2132 a         2573 c         2268 g         2306 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 aacgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggtt accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt cccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagatccat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tcctgtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttaccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata acctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgacccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctacgcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggtg tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaagcaatt atactgtata
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcactgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgtctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc tcttgtaaat agctactgta
     6121 ctcgcgccag cgccacggct tgtcctaact gccagaccgc cattgcgtaa tgggtgctgt
     6181 ggttgtaacg ggtgatggtg tagaagttcg gcagaccgta ccagtactgg tagccggtgc
     6241 caacatccag acgcagcagg ctggcttgtt gatggttgcc cagcggctgc tgtggcgtta
     6301 aacctgcggc ggcaagctgc gaaatgctgt acttagtttt gaaaccattt ggcaagcctg
     6361 gagcctgacc gtttgccatt atcgcgacct gatcgccttt cacccagccg tgcgctttga
     6421 aatagttcgc cacgctaccg atcgcatcaa ccggatccca caggttgata tgcccgtcgc
     6481 cgctgaaatc taccgcatat tgtttgtaag acgacggcat aaactgtccg tagcccatcg
     6541 ccccggcaaa ggaacctttc agattgagcg gatcgtcctg ctcgtcgcgc gccatcagca
     6601 ggaaggtttc cagctcgcca gagaaatact ccgcgcggcg tgggtagtta aatgacagcg
     6661 ttgccagcgc atcgaggatg cgagttttcc ccatcacgcg cccccagcgg gtttcaacgc
     6721 cgataatccc gacgataatt tccggcggta cgccatacac ctgccacgcg cgattcaacg
     6781 catcttcata ctgattccag aaaaccacac cgttctgcac gttgtccggc gtaataaatt
     6841 ttttgcgata acgcagccat gcgccgttcg gacctgatgg cggtttcacc gatgtggttg
     6901 gtgcctggtt atccatcagc cgcagtaccg aatccagacg cttcgcctgg gagagaattt
     6961 cctgcaactg ctgacgatcg aaaccgtgtt tgttcaccat tttgtcgatg aactgctggg
     7021 cattcgggtt attagcgaaa tcgccgccca tctgcatcac attgtgctgc ggctcaagca
     7081 ggaagccgcc agacggcgtt ccggtggtcg tttcagtctc agtaggtttt ggcttgctgc
     7141 tacaggcggc aagcaacaca aaaaggggaa gcaatgttac ataacgacgc ttgaacatga
     7201 ggggtccatt taacagattc aaccaggggc aagtatggta aagcatcacg ccccgcacaa
     7261 ggaagcggta gtcactgccc gatacggact ttacataact caactcattc ccctcgctat
     7321 acttttattc aaactttcaa attaaaatat ttatctttca ttttgcgatc aaaataacac
     7381 ttttaaacct ttcaatctga ttagattagg ttgccgtttg gtaataaaac aataaatcct
     7441 gaaggagaga acaatgatag aaaccattac tcatggtgca gagtggttta tcgggctgtt
     7501 ccaaaagggc ggagaggtgt ttaccgggat ggtgaccggc attcttccgc tgttgattag
     7561 cctgctggtt atcatgaacg cactgattaa ttttatcggt cagcatcgta ttgaacgttt
     7621 tgctcaacgt tgcgccggta accctgtttc ccgttaccta ctgttaccgt gcattggcac
     7681 gtttgtcttt tgcaatccga tgaccttaag cctgggtcgc tttatgccgg aaaagtacaa
     7741 acccagctac tacgcggcgg cctcttatag ctgccactca atgaatggcc tcttccccca
     7801 tatcaaccct ggcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcataa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctggcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcctgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcac
//
LOCUS       DADLSA010000149         9105 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.150, whole genome shotgun sequence.
ACCESSION   DADLSA010000149 DADLSA010000000
VERSION     DADLSA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.150"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            45..1103
                     /locus_tag="JN691_003415"
     CDS             45..1103
                     /locus_tag="JN691_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HAY7244765.1"
                     /translation="MEEDFRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVG
                     GSYHLAESYPPVHYIATDNYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYA
                     FRQLVAEEKYRGVVYQGLETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQ
                     VCEHLHIPVPEKLCVIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(1211..2035)
                     /locus_tag="JN691_003416"
     CDS             complement(1211..2035)
                     /locus_tag="JN691_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAY7244766.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            2355..4385
                     /gene="malS"
                     /locus_tag="JN691_003417"
     CDS             2355..4385
                     /gene="malS"
                     /locus_tag="JN691_003417"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY7244767.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            4563..5816
                     /gene="avtA"
                     /locus_tag="JN691_003418"
     CDS             4563..5816
                     /gene="avtA"
                     /locus_tag="JN691_003418"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAY7244768.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(5967..6440)
                     /locus_tag="JN691_003419"
     CDS             complement(5967..6440)
                     /locus_tag="JN691_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7244769.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(6542..7390)
                     /gene="yiaJ"
                     /locus_tag="JN691_003420"
     CDS             complement(6542..7390)
                     /gene="yiaJ"
                     /locus_tag="JN691_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAY7244770.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            7591..8589
                     /gene="yiaK"
                     /locus_tag="JN691_003421"
     CDS             7591..8589
                     /gene="yiaK"
                     /locus_tag="JN691_003421"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAY7244771.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            8601..9068
                     /locus_tag="JN691_003422"
     CDS             8601..9068
                     /locus_tag="JN691_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY7244772.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGQEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
BASE COUNT         2303 a         2319 c         2266 g         2217 t
ORIGIN      
        1 taggggaata tttacaggca tcacaatcag aatgggatat tttcattgaa gaagatttcc
       61 gcgcccgcat tgataaaatc aaggactggt taggagatgt cgtcattgcc gacttcgacg
      121 acaaacagat cgagcaagcg ctggctgatg tcgacgtccc cattgttggg gttggcggct
      181 cgtatcacct tgcagaaagt tacccacccg ttcattacat tgccaccgat aactatgcgc
      241 tggttgaaag cgcatttttg catttaaaag agaaaggcgt taaccgcttt gctttttatg
      301 gtcttccgga atcaagcggc aaacgttggg ccactgaacg cgaatatgca tttcgtcagc
      361 ttgtcgccga agaaaagtat cgcggagtgg tttatcaggg gttagaaacc gcgccagaga
      421 actggcaaca cgcgcaaaat cggctggcag actggctaca aacgctgcca ccgcaaaccg
      481 ggattattgc cgttactgac gcccgagcgc ggcatattct gcaagtatgt gaacatctac
      541 atattcccgt accggaaaaa ttatgcgtga ttggcatcga taacgaagaa ctgacccgct
      601 atctgtcgcg tgtcgccctt tcttcggtcg ctcagggcgc gcggcaaatg ggctatcagg
      661 cggcaaaact gttgcatcga ttattagata aagaagaaat gccgctacag cgaattttgg
      721 tcccaccagt tcgcgtcatt gaacggcgct caacagatta tcgctctctg accgatcccg
      781 ccgttattca ggccatgcat tacattcgta atcacgcctg taaagggatt aaagtggatc
      841 aggtactgga tgcggtcggg atctcgcgct ccaatcttga gaagcgtttt aaagaagagg
      901 tgggtgaaac catccatgca atgattcatg ccgagaagct ggagaaagcg cgcagtctgc
      961 tgatttcaac caccttgtcg atcaatgaga tatcgcaaat gtgcggttat ccatcgctgc
     1021 aatatttcta ctctgttttt aaaaaagcat atgacacaac gccaaaagag tatcgcgatg
     1081 taaatagcga ggtcatgttg tagcgatgat gagaattgtc ggcgtcacat cagggaactc
     1141 agcacagcca cctgatgcga gcaaaagcct tatccgaccg acaaatcaga taaggctcga
     1201 ggaaatgaaa ttacatatgc gccgcgatta accgttggtt atcttggtac attgcgaaca
     1261 ggtagttgtt ataactcttc cctttggtcg agtagccctt cagcttgtga atcatcgctg
     1321 tggcagtcac ttcctgatcc gctttacgca gctgtgcacg cgatttacgg aacgaagagt
     1381 aagccgggtg cgtattcagg ttagtgacat aggcgctcac cgattctttg acagaactaa
     1441 actgtgagta ccctttcact ttacccggcg cattggtaca acgtcctttc atgcatttca
     1501 tgccgaacag attgttattg ttgcgcgcca gcttcgacgt tccccaaccg ctttctgctg
     1561 cagccatcgt cgccaccata ctggtaggga taatgtctac gcgttcaagc aaggtattcc
     1621 acgggatttt tcgcgtatta ccagaccact tcaccttgta gcgtttggcg atgtctttca
     1681 gacgcgcacg ctcagcaggt gaccattggc cctgatactg ttttgaaatc agccagttac
     1741 gttctgcagt aatggctgca ttttggctgg taatgtaagg cattacggtc cggagaaacg
     1801 cctttttcct tggtgttccg gaagggtatt ttcgcaaatc aggaagtgaa ctactctttg
     1861 cactattgcg agaatactct tgtttactgc ttacctgttt attactagct ttagttaagt
     1921 gggacttttg actcgctgtt gttgtgtgcg tcttcgctaa cacctcactc gaaaacacca
     1981 gagtgagtaa cataagaatc atcgccccat atcgtcgtat gggagtcaaa atcatcaggt
     2041 ctcctggtcg gatttaatca ttccaacacc ttatattttt cacaaatttg agagttgaat
     2101 ctcaaatcat atcaaaaata gctgtcaaga gcaccccaag gaatagtcca aatctgaaac
     2161 tatgtcacga gttaacgatt cagattggcg ctaaatcgca gaaaatgtgg gggttatcgc
     2221 aaaattcagc cgttttttgc gcgcgatcgc tcacccttgc ttctcatcct gtggacttac
     2281 cgctcaggga tgagttttgt ctggcttatc gctggcaaac tgtctgaaat cgcagcaata
     2341 aggactcatc caccatgaaa ctcgccgcct gttttctgac actccttcct ggcttcgccg
     2401 ttgccgccag ctggacttct ccggggttcc ctgcctttag cgaacaggga acgggaacat
     2461 ttgtcagcca cgcgcagttg cccaaaggta cgcgtccact cacgctaaat tttgaccaac
     2521 agtgctggca gcctgcagat gcgataaaac tcaatcagat gctttccctg caaccttgta
     2581 gcaacacgcc gcctcaatgg cgattgttca gggacggcaa atatacgctg caaatagaca
     2641 cccgctccgg tacgccaaca ttgatgattt ccatccagaa cgccgccgaa ccggtagcaa
     2701 acctggtccg tgaatgcccg aaatgggatg gattaccgct cacgctggat gtcagcgcca
     2761 ctttcccgga aggagccgcc gtacgggatt attacagcca gcaaattgcg atagtgaaga
     2821 acggtcaaat aacgttacaa cccgctgcta ccagcaacgg tttactcctg ctggaacggg
     2881 cagaaactga cacatccgcc cctttcgact ggcataacgc cacggtttac tttgtgctga
     2941 cagatcgttt cgaaaacggc gatcccagta atgaccagag ttacggacgt cataaagacg
     3001 gtatggcgga aattggcact tttcacggcg gcgatttacg cggcctgacc aacaaactgg
     3061 attacctcca gcagttgggc gttaatgctt tatggataag cgccccattt gagcaaattc
     3121 acggctgggt cggcggcggt acaaaaggcg atttcccgca ttatgcctac cacggttatt
     3181 acacacagga ctggacgaat cttgatgcca atatgggcaa cgaagccgat ctacggacgc
     3241 tggttgatag cgcacatcag cgcggtattc gtattctctt tgatgtcgtg atgaaccaca
     3301 ccagctatgc cacgctggcg gatatgcagg agtatcagtt tggcgcgtta tatctttctg
     3361 gtgacgaagt gaaaaaaacg ctgggtgaac gctggagcga ctggaaacct gccgccgggc
     3421 aaacctggca tagctttaac gattacatta atttcagcga caaaacaggc tgggataaat
     3481 ggtggggaaa aaactggatc agaacggata tcggcgatta cgacaatcct ggattcgacg
     3541 atctcactat gtcgctggcc tttttgccgg atatcaaaac cgaatcaact accgcttctg
     3601 gtctgccggt gttctataaa aacaaaacgg atactcacgc taaagtcatc gaaggcttta
     3661 cacctcgcga ttacttaacc cactggttaa gtcagtgggt ccgcgactat gggattgatg
     3721 gttttcgggt cgataccgcc aaacatgttg agttgcccgc ctggcagcaa ctgaaaaccg
     3781 aagccagctc cgcgcttcgc gaatggaaaa aaactaaccc cgacaaagca ttagatgaca
     3841 aacctttctg gatgaccggt gaagcctggg gccacggcgt gatgcaaagt gactactatc
     3901 gccacggctt cgatgcgatg atcaatttcg attatcagga gcaggcggcg aaagcagtcg
     3961 attgtctggc gcagatggat acgacctggc agcaaatggc ggagaaattg cagggtttca
     4021 acgtgttgag ctacctctcg tcgcatgata cccgtctgtt ccgtgaaggg ggcgacaaag
     4081 cagcagagtt attactatta gcgccaggcg cggtacaaat cttttatggc gatgaatcct
     4141 cgcgtccgtt cggtcctaca ggttctgatc cgctgcaagg tacacgttcg gatatgaact
     4201 ggcaggatgt tagcggtaaa tctgccgcca acgtcgcgca ctggcagaaa atcagccagt
     4261 tccgcgcccg ccatcccgca attggcgcgg gcaaacaaac gacactttcg ctgaagcagg
     4321 gctacggctt tgttcgtgag catggcgacg ataaagtgct ggtcatctgg gctgggcaac
     4381 agtgactttt ccggcttccc gttcgtcagt acctcgggaa gccgccaacc aggataaaat
     4441 gtcagcccta atcagcgttg cgggataaag caccgctcac tcttcaacag accgatttgc
     4501 accccagcaa atgtagcgtt attgttacct tccttgctac agagttcgac agatatcccg
     4561 ctatgacatt ctcccttttt ggtgacaaat ttacccgcca ctccggcatt acgctgctga
     4621 tggaagatct gaacgacggt ttacgcacgc ctggcgcgat tatgctcggc ggcggtaatc
     4681 cggcgcagat cccggaaatg caggactact tccagacgct actgaccgac atgctggaaa
     4741 gtggcaaagc gactgatgca ctgtgtaact acgacggtcc acaggggaaa acggagctac
     4801 tcacactgct tgccggaatg ctgcgcgaga agttgggttg ggatatcgaa ccacagaata
     4861 ttgcactaac aaacggcagc cagagcgcgt ttttctactt atttaacctg tttgccggac
     4921 gccgtgccga tggtcgggtc aaaaaagtgc tgttcccgct ggcaccggaa tacattggct
     4981 acgctgacgc cggactggaa gaagatctgt ttgtctctgc gcgtccgaat attgaactgc
     5041 tgccggaagg ccagtttaaa taccacgtcg attttgagca tctgcatatt ggcgaagaaa
     5101 ccgggatgat ttgcgtctcc cggccgacga atccaacagg caatgtgatt actgacgaag
     5161 agttgctgaa gcttgacgcg ctggcgaatc aacacggcat tccgctggtg attgataacg
     5221 cttatggcgt cccgttcccg ggtatcatct tcagtgaagc gcgcccgcta tggaatccga
     5281 atatcgtgct gtgcatgagt ctttccaagc tggggctgcc tggctcccgc tgcggcatta
     5341 tcatcgccaa tgaaaaaatc atcactgcca tcaccaatat gaacggcatt atcagcctgg
     5401 cacctggcgg tattggtccg gcgatgatgt gtgaaatgat taagcgtaac gatctgctgc
     5461 gcctgtctga aacagtcatc aaaccgtttt actaccagcg tgttcaggaa actatcgcca
     5521 tcattcgccg ctatttaccg gaaaatcgct gcctgattca taaaccggaa ggagccattt
     5581 tcctctggct atggtttaag gatttgccca ttacgaccga gcagctctat cagcgcctga
     5641 aagcacgcgg cgtgctgatg gtgccggggc acaacttctt cccgggactg gataaaccgt
     5701 ggccgcatac gcatcaatgt atgcgcatga actacgtacc agagccggag aaaattgagg
     5761 cgggggtgaa gattctggcg gaagagatag aaagagcctg ggctgaaagt cactaaaaat
     5821 tgcctgatgc gctacgctta tcaggcctac atgatttctg caatatattg aatttggaag
     5881 aatttgtagg ccggataagg cgtttacgcc gcatctggca taaacaaagc gcactattcc
     5941 ggcgttgaga aacgccggaa tacgtttcaa acaggctgct gctgtagccg atgccgctgc
     6001 actttctcga catccatgca ctgcaacgcc tttgtcgggc aggcttcaac acaggccggc
     6061 cccgtctccc gatgccagca cagatcgcac ttaatcgccc tcgccttttt gcgcgaagaa
     6121 acgacctcca tcgcaccaaa cgggcaagcc agcatacagc ttttacagcc aatgcaacgt
     6181 gtttgttcaa cgaaaatatg cccatgttcg cggcttatcg cgtcaacagg gcagacattc
     6241 gcgcacggtg catcttcaca ctgatgacag gctactggcg tggtccagct gtggtcttta
     6301 atgacacgaa tacgggaaat aaactcgtct ggtgacaacg cagcgcaatc ctgattctcc
     6361 tgatgcgaca ctgcgcaagc cacttcacag gtacggcaac cgatacattt cgtcgcatcc
     6421 gcaataataa accggttcat gatcactcct ttgcactaaa acagcagagt gccagagagc
     6481 gcgtccctac tgctttgatc caacgaagga aatgttaaag ttttggcact tgtccaaagc
     6541 gttatgtgat cgcgcccagg tcatccctga cagtaaatcc cagttcatta gaaatagcct
     6601 gcgcggtttc acgcagtggt ttcaggagat ttttctctcc cacctgtttc agacgtgatg
     6661 tcgaaagcga aatcgacacg gcgtacggca cccgcccatg aatatcaaac accggaacag
     6721 caatacagga gacgccgagt tcgttttctt ctctgtccat cgccgctccg ctttcacgaa
     6781 tgtgcgccag ttcgtcgaac atcgcgggca gctcggtaat ggtattgcgg gttaacggct
     6841 ggatctcatg ctgatggttt tcccagtatg acttcacgta gtccgggtga ccaaacgcca
     6901 tgtagatctt gcccattgcg gaacagtaga gcggcatatg ctggccaata taggcacggg
     6961 ttcgcagcat cccggttgtg ggttccagct tataaatcaa aatagcgtga tcgtcttcgc
     7021 ggctggagaa gttaatggtt tcaccagtgg cgatgttcag tgcctcaaga tgcggagcgg
     7081 cgatatgaat gatattcagc gaagacagcg ccttctgccc gacggcaata aatttggtag
     7141 tcaggcggta actccctgcg gcgggcgcgg tggtcacata gccgcaggac tgtaatccct
     7201 gcaataagcg atggacggta ctcttattta aaccagccag ctccgaaaga tgcgccaacg
     7261 gacaaccgtt tggatagttg ctcaaaatct caatcagcat caacccgcga aacagactct
     7321 ggcttccggc tggacgctct ttttcctgcg ccatctcgtt ctcttttttt cccatcactt
     7381 ctttccccat tttgtcgcgt cctgatggta gcgcaaagtg tgccgtagtt cacgatctcg
     7441 acagataatt tataaccact tgatttttat gtcttttgaa attcatcaat cagattgctt
     7501 tgttaaaaag tgatcgatat atttgaaatc tagtttcgta tattgaaatt ttaagccaaa
     7561 aaagcgatca aaaaaacaag gaagcctggg atgaaagtga catttgagca gttaaaagca
     7621 gcctttaatc gggtcttaat ttcacgcggc gttgacagcg aaacggctga cgcctgtgca
     7681 gagatgttcg cccgcaccac cgaatccggc gtttattctc acggcgttaa tcgtttccct
     7741 cgtttcattc aacaactgga aaacggcgat atcattcctg atgcccaacc caaacgtata
     7801 accagcctcg gcgcaattga acagtgggat gcccagcgtt caatcggcaa cctgacggcg
     7861 aaaaagatga tggatcgcgc cattgaactg gctgccgatc acggtattgg tctggtggca
     7921 cttcgtaatg ccaaccactg gatgcgcggc ggcagctacg gctggcaggc ggcggaaaaa
     7981 ggctatattg gcatttgctg gaccaactcc atcgccgtaa tgccgccgtg gggcgcaaaa
     8041 gagtgtcgca tcggcactaa cccgctgatc gtcgccattc cttccacgcc gatcaccatg
     8101 gtcgatatgt cgatgtcgat gttctcttac ggcatgttag aagttaaccg cctggcaggc
     8161 cgtcagctcc cggtcgatgg tggctttgat gatgagggca atttgaccaa agaacctggc
     8221 gttatcgaga agaatcgccg cattttgccg atgggctact ggaaaggttc tggcatgtcg
     8281 attgtgctgg atatgatcgc tactctcctt tccgacggcg catcggttgc cgaagtcacc
     8341 caggacaaca gcgacgaata cggcatttca caaattttta ttgccattga agtggacaag
     8401 cttatcgacg gtcccacccg cgatgccaag ctgcaacgta tcatggatta cgttactacc
     8461 gctgaacgcg ctgacgaaaa ccaggccatc cgcttacccg gccacgaatt tactaccctg
     8521 ctggccgaaa accgccgtaa cggcatcact gttgatgaca gcgtgtgggc caaaatccag
     8581 gcgttatgag gagataagtc atgatttttg gacatatcgc gcagcccaat ccgtgccgtt
     8641 tgcccgccgc cattgaaaag gcgctcgatt ttctgcgcgc taccaacttc aacgtcctgg
     8701 agccgggcgt tgtcgaaatc gacggcaaga atatttatgc gcaaattatc gatttaacca
     8761 cacgcgaagc ggtggaaaac cgtccggaag tgcatcgtcg gtatatcgat attcagtttc
     8821 tggcgtgggg tcaagagaaa atcggcattg ctattgatac aggaaataat aaagtcagcg
     8881 aatcactatt agagcagcgc gatattattt tttatcatga cagtgaacat gaatcattta
     8941 tagaaatgat accgggcagc tacgctattt tcttcccgca ggatgttcac cgaccaggtt
     9001 gtattctgca aacagcctct gagatacgaa aaattgtggt taaagtcgca ttaacagcgc
     9061 tgaattaatc aaataatcgt ctacgccaga acgactgggt aatgt
//
LOCUS       DADLSA010000150         8969 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.151, whole genome shotgun sequence.
ACCESSION   DADLSA010000150 DADLSA010000000
VERSION     DADLSA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.151"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..282
                     /locus_tag="JN691_003423"
     CDS             <1..282
                     /locus_tag="JN691_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traD domain protein"
                     /protein_id="HAY7244773.1"
                     /translation="PVSPVINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISE
                     SGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(291..689)
                     /gene="vapC"
                     /locus_tag="JN691_003424"
     CDS             complement(291..689)
                     /gene="vapC"
                     /locus_tag="JN691_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HAY7244774.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(689..916)
                     /gene="vapB"
                     /locus_tag="JN691_003425"
     CDS             complement(689..916)
                     /gene="vapB"
                     /locus_tag="JN691_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HAY7244775.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            1070..1417
                     /locus_tag="JN691_003426"
     CDS             1070..1417
                     /locus_tag="JN691_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005056182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase domain-containing protein"
                     /protein_id="HAY7244776.1"
                     /translation="MLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSR
                     MQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIEAHNQAVDFAVRHHPCGGTGLHT
                     GDDGRTVRNGADR"
     gene            1404..6275
                     /gene="traI"
                     /locus_tag="JN691_003427"
     CDS             1404..6275
                     /gene="traI"
                     /locus_tag="JN691_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HAY7244777.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQHNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQVIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQGETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQA
                     IAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQV
                     PRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTP
                     EAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVPVLNTANIRDGELRRLST
                     WENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTI
                     RVGTGDRIRFTKSDRDRGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHID
                     LAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGW
                     TDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDS
                     PARFIAPGRKYPQPYVALPAFDRNGKSTGIWLNPLTTDDGNGLRGFSGEGRVKGSGDA
                     QFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGD
                     IPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTE
                     QAVREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDM
                     VRDLQKEKTPGGD"
     gene            6295..7041
                     /gene="traX"
                     /locus_tag="JN691_003428"
     CDS             6295..7041
                     /gene="traX"
                     /locus_tag="JN691_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAY7244778.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMSLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            7096..7656
                     /locus_tag="JN691_003429"
     CDS             7096..7656
                     /locus_tag="JN691_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAY7244779.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7787..7999
                     /locus_tag="JN691_003430"
     CDS             7787..7999
                     /locus_tag="JN691_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY7244780.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8183..8704
                     /locus_tag="JN691_003431"
     CDS             8183..8704
                     /locus_tag="JN691_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HAY7244781.1"
                     /translation="MLHYSGGLKYRCHLSDMENNMRKYIPLVLFIFSWPVLSADIHGR
                     VVRVLDGDTIEVMDSLKAVRIRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQ
                     RDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSD
                     ADPVPPWIWRHRK"
     gene            8750..8959
                     /gene="hha"
                     /locus_tag="JN691_003432"
     CDS             8750..8959
                     /gene="hha"
                     /locus_tag="JN691_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY7244782.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
BASE COUNT         2116 a         2231 c         2828 g         1794 t
ORIGIN      
        1 ccggtgtctc ctgtcatcaa cgataagaag tcagattcag gtgtgaatgt tccggcaggg
       61 gggatcgagc aggagctgaa aatgaaaccg gaagaagaga tggaacagca actgccaccc
      121 gggatcagtg aatccggtga agtggtggat atggccgctt atgaggcatg gcagcaggaa
      181 aatcatccgg acacctggca gcagatgcag cgtcgtgaag aggtgaacat caatgtgcac
      241 cgggaacgcg gggaggatgt tgagccggga gatgatttct gatgagcagg tcagctccag
      301 tcttcagttc tcaggccgcc cacacgttca aattcccggg tgttattagt cacaataatc
      361 agcccccgac tgcgggcatg acctgcgatc atttgatcaa atggcccgac agggcgtcct
      421 tgacgggcaa gttctgctct tatctggccg gtgtgtgtgg ctgcagcagc gtcgtaatcc
      481 agaacatcaa tgcgggaaac aaatccctcg atcacagcga gattacgttc aggcatctgg
      541 cttttttctg caccatatat cagctccatc agggtgaccg aactgataca cattctcccc
      601 tggttcaggt taaaacgctc cctgacgctg gcgggtttgt tctttatcgt aaaaatgcag
      661 atgttggtat cgagcataaa cttcagcatc agaatgactc cctttcctgc atgccgggct
      721 gttccctgtt atccataaaa tcggcgctga cgctgtgtcc gtcgaaccat tcgtcccatg
      781 tctctccggc tggcgtaatg attctggttc gtccgacagc aatcacttca acgcgtttta
      841 cgttttccgg caatgcaacc gcttttggca gtctgaccgc ctggctgcgg ttgctgagaa
      901 atacggtggt ttccattatt tgcctcctta tgtggatatg tcttgtgtat atctattgtg
      961 ggattgatac cgggatatgt caaagggata tacgtttatg atgagtatcg cgcaggtcag
     1021 atcggccgga agtgccggta attattatac cgacaaggat aattactatg tgctgggcag
     1081 catgggagaa cgctgggccg gccagggggc tgaacaactg gggctgcagg gcagtgtcga
     1141 taaggatgtc tttacccgcc ttctggaggg caggctgccg gacggggcgg atttaagccg
     1201 catgcaggat ggcagtaaca ggcatcgtcc cggctacgac ctgaccttct ccgctcccaa
     1261 aagtgtctcc atgatggcca tgctcggggg ggacaaacgt cttattgaag cacataacca
     1321 ggccgtggat tttgctgtcc gtcatcaccc gtgtggaggc actggcctcc acacgggtga
     1381 tgacggacgg acagtcagaa acggtgctga ccggtaatct ggtgatggca ctgtttaacc
     1441 acgacaccag tcgcgatcag gaaccacagt tacacacgca tgcggtggtg actaatgtta
     1501 cgcagcataa cggcgaatgg aagacactga gcagtgacaa agtggggaaa acggggttca
     1561 ttgagaatgt gtacgctaat cagattgcct ttggcaggct ctaccgggaa aaactgaaag
     1621 agcaggttga ggcgctgggc tatgaaactg aagtggtcgg taaacacggt atgtgggaaa
     1681 tgccgggtgt accggtggag gccttttccg gacgctcaca gactatccgg gaggccgtcg
     1741 gggaagacgc ctcgctgaaa tcccgggatg tggcggcgct ggatacgcgt aaatccaaac
     1801 agcacgtcga tcctgaagtc agaatggccg agtggatgca gacgctgaag gaaaccgggt
     1861 tcgacatccg ggcatatcgt gacgcagcgg aacaacgggc atatacccgc acgcagacac
     1921 ccggacccgc ttcacaggac gggccggatg tgcagcaggc ggtgacacag gccattgccg
     1981 gattaagtga acgcaaagtg cagtttatgt atacggacct gctggccagg acggtcggca
     2041 tactgccgcc ggaaaatggt gtgattgaac gggcacgcgc cggtatcgat gaggccatca
     2101 gccgtgagca gcttatcccc ctcgatcgtg agaaggggct gttcacgtcc gggattcata
     2161 tgctcgatga gctgtcagtc cgggcactca gtcgtgacat catgaaacag aaccgggtga
     2221 ccgtacatcc ggagaaaagt gtcccccgga cggccggtta cagcgatgcc gtcagtgttc
     2281 tggcacagga ccgtccgtcg ctggccattg tgtccgggca gggcggtgca gccgggcagc
     2341 gtgagcgggt ggctgaactg gtcatgatgg cccgggagca ggggcgggag gtgcagatta
     2401 tcgctgctga ccgtcgctcg cagatgaacc tgaagcagga tgaacggctg tccggtgaac
     2461 tgataaccgg acgtcgtcag ttgctggaag gcatggcctt cacgccgggc agtaccgtta
     2521 tcgtcgacca gggcgaaaaa ctctccctga aagagacgtt aaccctgctg gatggtgccg
     2581 cacgtcataa cgtacaggtc ctgataaccg acagcgggca gcgaaccggt acaggcagtg
     2641 cactgatggc catgaaggat gccggggtga acacatatcg ctggcagggg ggagaacagc
     2701 gaccggccac catcatcagt gaaccggacc gtaatgtccg ctatgcccgg ctggccggag
     2761 attttgcggc cagcgtgaaa gccggagaag agagcgtggc acaggtcagc ggagtacggg
     2821 aacaggccat actgacacag gtcattcgca gtgagctgaa aacacagggc gtgctcggac
     2881 acccggaggt aaccatgacc gccctgtcac cggtctggct ggacagccgg agccgttatc
     2941 tgcgggatat gtaccgcccg ggaatggtga tggagcagtg gaacccggag acacgcagtc
     3001 atgaccgcta tgtgacagaa cgtgtgacgg cgcagagtca cagcctgacc ctgcggaatg
     3061 cgcagggcga aacgcaggtg gtgcgtattt cttccctgga cagcagctgg tcgctgttcc
     3121 ggccggaaaa aatgccggtg gcagacggtg agcgtctgag ggtgacaggg aaaattcccg
     3181 gactccgcgt ctccggcggt gaccgcctgc aggtggcatc agtcagtgaa gatgcgatga
     3241 cggttgttgt gccgggacgg gctgaaccgg ccaccctgcc tgtgagcgat tcaccgttca
     3301 cggcactgaa gctggagaac ggctgggtgg aaacgcccgg gcattccgtc agcgacagtg
     3361 cgacggtttt tgcctccgtc acacagatgg caatggacaa tgccaccctg aacggtctgg
     3421 cccgcagtgg tcgtgatgtc cggctgtatt cctcactgga tgaaacccgt actgcggaaa
     3481 aacttgcccg ccatccctcc tttacggtgg tttctgagca gataaaggcg cgtgccggtg
     3541 agacattgct ggaaaccgct atcagtctgc agaaagccgg gcttcacacg ccggcacagc
     3601 aggccattca tctggccctt cctgtgctgg aaagtaaaaa cctggccttc agcatggtgg
     3661 acctgctgac agaggcgaag tcgtttgctg cagaaggaac cggttttgct gacctgggag
     3721 gggaaatcaa tgcgcagata aaacggggtg atttactgta tgtggatgtg gcaaaaggct
     3781 atggcacagg cctgctggtt tcccgtgcgt cgtatgaggc agaaaagagc attcttcgcc
     3841 atattctcga aggtaaggag gcggtcacgc cgctgatgga gagagtacct ggcgaactca
     3901 tggagaaact gacatcagga cagcgcgccg ccacccgtat gatactggaa acgtccgacc
     3961 gtttcacggt ggtgcagggc tatgccggtg tgggtaagac cacacagttc cgggcggtga
     4021 tgtcagccgt gaacatgctg ccggagagtg aacgtccccg tgtcgtgggg ctggggccca
     4081 cacaccgtgc ggtcggggag atgcgcagcg ccggcgtgga tgcacagaca ctggcgtcct
     4141 ttctgcatga cacgcagctg cagcagcgca gcggagaaac gccggatttc agcaacacgc
     4201 tgttccttct cgatgagagc tctatggtgg gcaataccga catggcacgg gcatacgccc
     4261 tgattgcggc cggtggcggt cgggctgtgg ccagcggtga cacggaccag ctgcaggcca
     4321 tcgcgcccgg tcagcctttc cgtctccagc agacgcgcag tgctgccgat gtggtcatca
     4381 tgaaggagat tgtgcgtcag acgccggaac tgcgggaggc ggtatacagc ctgattaacc
     4441 gggatgtgga aagggcgctg tccgggcttg agcgtgtgaa accgtctcag gtgccacgtc
     4501 tggagggcgc atgggcaccg gagcactccg tgacggagtt cagtcacagc caggaagcga
     4561 aactggcaga agcgcagcag aaggcgatgc tgaaaggcga ggcttttccg gatgtcccca
     4621 tgacactgta tgaagccatt gtccgcgact ataccggcag gacaccggaa gcacgggagc
     4681 agacgctgat tgtcacgcac ctgaatgagg accggcgcgt actgaacagc atgattcatg
     4741 atgcacggga aaaggccgga gaactggggc aggtgcaggt catggtgcct gtcctgaaca
     4801 cagcgaatat acgcgacggg gagctgcgtc gtctctccac ctgggagaat aacccggatg
     4861 cccttgccct ggtggataat gtgtatcacc ggattgccgg tatcagcaag gatgacgggc
     4921 tgataaccct gcaggatgcg gaaggtaaca cgcggctgat ttcgccccgg gaggcggtgg
     4981 ctgaaggcgt cacgctgtac accccggaca ccatccgggt ggggaccggt gacaggatac
     5041 gcttcacgaa gagtgaccgg gatcgcggtt atgtggccaa cagcgtctgg acggtgacag
     5101 cggtttccgg tgacagtgtc acactttctg acggtcagca gacccgtgtg attcgccccg
     5161 gtcaggagcg ggcagagcaa catattgacc tggcctatgc catcaccgcc cacggtgcac
     5221 agggggcgag tgaaaccttt gccatcgcgc ttgaaggcac ggaaggtaac cggaaactga
     5281 tggccggctt tgagtcagcc tacgtggccc tgtcgcgtat gaagcagcat gtgcaggtgt
     5341 acaccgataa ccgtcagggc tggacggatg ccatcaacaa tgccgtacag aaaggaactg
     5401 cccacgatgt gtttgagccg aaaccggacc gggaggtcat gaatgcagag cggctgttca
     5461 gtacggcgcg ggagctgcgg gacgtggcgg caggccgtgc cgttctccgt caggcggggc
     5521 tggccggggg agacagtcct gcacggttta ttgctccggg acgtaaatat ccgcaaccgt
     5581 atgtggcact gccggcgttt gaccgtaacg gcaagtccac cggtatctgg ctgaacccgc
     5641 tgaccacgga tgacggaaac gggctgcggg gattcagcgg tgaaggtcgg gtaaaaggca
     5701 gcggggatgc gcagttcgtg gccctgcagg gcagccgtaa cggagaaagc ctgctggctg
     5761 ataatatgca ggatggcgtc cggattgccc gtgataatcc tgacagtggt gtggtggtaa
     5821 gaatcgccgg tgaaggccgt ccgtggaatc ccggtgccat caccggtggc cgcgtgtggg
     5881 gggatatccc ggacaacagc gtccagccgg gagccggaaa tggcgaaccg gtcacggcag
     5941 aggtactggc acagcggcag gctgaagagg ccatccgccg tgaaacggaa cgtcgcgcag
     6001 atgaaattgt ccgtaaaatg gcagagaaca aacctgacct gccggacggc aaaacagagc
     6061 aggctgtcag ggaaattgcc gggcaggagc gtgaccgggc tgccataact gaacgggaag
     6121 ccgcgctgcc ggagagtgta ctgcgtgaac cacaacgggt gcgggaagcg gtccgggaag
     6181 ttgcccggga aaatttgctg caggagcgtc tgcagcagat ggagcgggat atggttcgtg
     6241 acctgcagaa agagaaaacc ccgggcggag actgatacag gaagacaaac actgatgaca
     6301 acggataaca cgaacacgac acgtaacgat tcactggctg cacggaccga tacctggttg
     6361 cagtcatttc tggtctggtc acccggacag cgggatatca tcaaaacggt ggcactggtg
     6421 ctgatggtac tggaccacat caatctgata ttccagctga agcaggaatg gatgtctctg
     6481 gccgggcgag gagcctttcc gctttttgcc ctggtgtggg ggctgaatct gtcccgtcat
     6541 gcgcatatcc ggcaaccggc cattaaccgg ctgtggggat ggggaatcat tgcccagttc
     6601 gcatattatc tggcaggctt tccctggtat gaggggaata tcctgtttgc ctttgcagtg
     6661 gcggcacagg tgctgacgtg gtgtgaaacg cgcagtgggt ggcgtacagc agcggccata
     6721 ctcctgatgg ccctgtgggg gcctttgtcc ggcaccagtt acggtattgc cgggttgctg
     6781 atgctggcag tcagcaaccg gctgtaccgg gcggaagaca gagcggaacg tctggcgctg
     6841 gtcgcctgcc tgctggctgt tattccggcg cttaaccttg ccaccagtga tgcagcggcg
     6901 gtggccggtc ttgtgatgac ggtgctgacc gttggtctgg tgttgtgtgc cggaaaatca
     6961 ttaccccgtt tctggcccgg agactttttc ccgacgttct atgcctgtca tctggctgtg
     7021 ctgggcgttc tggcgctgtg acgggtgtgg tatctttggc cgcaagagga tgatttgtca
     7081 gaggcagaac acagcatgac agagcagaag cgaccggtac tgacactgaa gcggaagaca
     7141 gagggaacag cgcctgtccg cagccggaaa accatcatca atgtcaccac gccaccaaag
     7201 tggaaggtga aaaagcagaa gctggccgag aaagcggccc gtgaagcaga gctggcggca
     7261 aaaaaagcgc aggccagaca ggcgctgtcc atttatctga acctgcccac gctggatgag
     7321 gctgtgaata ccctgaagcc ctggtggccg ggattatttg acggtgacac gccccggctt
     7381 ctggcctgcg gtatccggga cgtgttactg gaagacgtgg cgcagcggaa tatcccgctc
     7441 tcgcataaaa aactgcgcag ggcgctgaag gccatcaccc gttcagaaag ctatctgtgt
     7501 gccatgaaag ccggtgcctg ccggtatgac acggaagggt atgtgacgga gcatatttct
     7561 caggaggagg aagcgtatgc gggagcgcgt ctggcgaaaa ttcgtcgcca gaaccggata
     7621 aaggcagaac ttcaggccgt gcttgatgag aaataaaaaa agcctccccc agagagagga
     7681 ggcagggaaa taaggcttaa aaggaaataa cttccgcttc cgaaaggaag ccagtccact
     7741 gaattattta tcacaataat actgacaact aaagggggac aagaaaatga atggattcag
     7801 aaacagttca cggaacggcc aggtctggcg ttaccagcgt gccggcgggc gggccgttat
     7861 tctggaagtc agtggacgct ggatggaagc ggcagaagca tggcgacggg ctgcctgtgt
     7921 tgcgcctagg acagactggc agcagtttgc ccggaaaagg gctgaacact gccaccggcg
     7981 ctgccgggga agggtgtaac gaaaaaagca gccttcatta ctccaggagt cagaaggctg
     8041 aaaggagttt atgagatttg cttcttaata tccggtcggg cagtatgacc ggacagttct
     8101 gagtagcaca gagtcttttc cgcattaagt cgatttttta tattttgtga ttaatttcac
     8161 aaaataaggt gttattcaat gtgtgctgca ctattcaggg ggtctgaaat acaggtgcca
     8221 tttatctgat atggagaata acatgaggaa atatattcca ctggtattat ttatcttttc
     8281 atggccggta ttgtctgcag atattcatgg acgggttgtt cgtgtactgg acggtgatac
     8341 catcgaggtc atggactcac tgaaggcggt gcggatcaga ctggtaaata ttgatgcacc
     8401 ggaaaagaaa caggattatg gccggtggtc aacagacatg atgaaatcac tggtcgcagg
     8461 aaaaacggtt accgtcacat attttcagcg agatcgttat ggtcgtattc tcgggcaggt
     8521 ttatgcacca gacggaatga atattaatca gtttatggtc cgggccgggg ctgcctgggt
     8581 atatgagcag tacaacacag atcccgtgct acctgtcctt cagaacgaag ccagacaaca
     8641 aaaacgtggg ctctggtcag atgctgatcc ggttcccccc tggatatgga ggcaccgtaa
     8701 ataaagcgat ggcaatccta tcattcatct gttctcagcg ggaataaaaa tggcaaaaac
     8761 aaaacaggaa tggctgtatc agttacggcg atgttcatca gtcaatacgc tggaaaaaat
     8821 tattcataaa aacagagaca gtttatcaaa cagtgaacgt gaatcattta attcagcagc
     8881 agaccaccgg ctagcggaat taatcaccgg aaagttatat gacaggatac caaaagagat
     8941 atggaaatat gtaaggtaat aattcagca
//
LOCUS       DADLSA010000151         8964 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.152, whole genome shotgun sequence.
ACCESSION   DADLSA010000151 DADLSA010000000
VERSION     DADLSA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.152"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(132..1733)
                     /gene="tap"
                     /locus_tag="JN691_003433"
     CDS             complement(132..1733)
                     /gene="tap"
                     /locus_tag="JN691_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HAY7244783.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGILTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLERPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQESATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(1779..3440)
                     /gene="tar"
                     /locus_tag="JN691_003434"
     CDS             complement(1779..3440)
                     /gene="tar"
                     /locus_tag="JN691_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HAY7244784.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(3585..4088)
                     /gene="cheW"
                     /locus_tag="JN691_003435"
     CDS             complement(3585..4088)
                     /gene="cheW"
                     /locus_tag="JN691_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HAY7244785.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(4109..6073)
                     /gene="cheA"
                     /locus_tag="JN691_003436"
     CDS             complement(4109..6073)
                     /gene="cheA"
                     /locus_tag="JN691_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HAY7244786.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSTEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFALGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(6078..7004)
                     /gene="motB"
                     /locus_tag="JN691_003437"
     CDS             complement(6078..7004)
                     /gene="motB"
                     /locus_tag="JN691_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HAY7244787.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPELRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVRVPTMPSAEPR"
     gene            complement(7025..7888)
                     /gene="motA"
                     /locus_tag="JN691_003438"
     CDS             complement(7025..7888)
                     /gene="motA"
                     /locus_tag="JN691_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HAY7244788.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSGETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQ"
     gene            complement(8015..8593)
                     /gene="flhC"
                     /locus_tag="JN691_003439"
     CDS             complement(8015..8593)
                     /gene="flhC"
                     /locus_tag="JN691_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HAY7244789.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(8596..8946)
                     /gene="flhD"
                     /locus_tag="JN691_003440"
                     /pseudo
     CDS             complement(8596..8946)
                     /gene="flhD"
                     /locus_tag="JN691_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
BASE COUNT         2036 a         2474 c         2209 g         2245 t
ORIGIN      
        1 ctattgccag tacacctcag acttatccgc cgaaaatgcg cgtcggaaag cgccaggcgc
       61 tcggtcatct gtaacaataa agacgtttgc ccacagggca gagatgaagt catagcgcct
      121 tctcaatcac ttcaggatac cactggcgca atttgtaact gcgccgactc atgtcgtgct
      181 acttcatgtt cttcaagggt aaataccgcc acacgcgacg aaagatggtc ggcctgattc
      241 gccagttgtt ccgttgccac cgccgcttct tctaccagcg aggagttctg ctgagtcacc
      301 tgatccatct ggctgacagc ctgtgcaacc tgctcaatcc cccgccgttg ttcttccgac
      361 gccgaagcaa tttctcccat aatgtcgttc acgcgggtca ccgaactgac aatatcggtc
      421 atggtcgcgg cggcgttatt caccagtttc gaaccctgct ggacacgatt gactgactct
      481 tcgatcagcc ctttgatctc ttttgctgcc tgcgcgctac ggctggcaag attgcgcact
      541 tcacctgcca ctaccgcaaa accacgcccc tgctctccgg cgcgagccgc ttccactgcc
      601 gcattcaggg ccagaatatt ggtctggaaa gcaattccgt cgataacgct gataatgtcg
      661 ccaatttttt gcgagctggt ggcgctctcc tgcatggtgt gagtcatggt actgacctga
      721 acaccgcccg cctgcgccgt tgtcgcggca ttttttgcca gttccgacgc ctgtcgtgcg
      781 ttatcggcgt tttgccctac cgtggcggtt aattgctcca tactggcggc cgtttgtgcc
      841 agcgatgccg cctgctgttc ggtacgactt gagagatcgt tattgcctgc gacaatctcc
      901 gcgatcccaa tgtgcatctc atggcttccc ttacgcacat cactcaccgt cccacgcaaa
      961 gcctgctgca tggtcttcag gctggcaaaa atggcggtga tctcattacg accatatacc
     1021 gcaatcggac gctccagatt acccgcagca atgctgtcaa aatggctacc gataatggcc
     1081 agtggttgaa caatcatctt gcgcgtccac cacagcgcac tgctgatgta gatcgccgca
     1141 acaataatca tgctgataaa caccagcgcc gaaatctgat agttacgctg gctttgcgca
     1201 ctggcggctt ccagcacatg gttgatctcc agctgccagg cctcaaagtt aacgtcaaac
     1261 gcattctgtg atccctgcac cggcgcagtg aggaaatccg aaagttgatt actttcaagc
     1321 caggtcgcct gatgttcgag atcgttgtgc cagcgggcaa aacttttctc cgtctctttt
     1381 tgcaatgccc tgacgtgctc gttgcccgca gtcatcgcca taaaactttt aaacagcgtg
     1441 gtggattgcg tcagactggc gcgcgccgtc gtcatcaacg ttttaatgtc atccgccgga
     1501 tagctaagcg ctgtcagaat gcctgctttg ttcagcgcgg tactggcctg taacattacc
     1561 gcccgagttt gcgctaatgc cgcacgttgc tgattgctct gctcgccgtg attcagtcgt
     1621 tgcaaatcgt cgcgaaatgc ccaaaaagac atgccgttac tgccaatctg caagatcccg
     1681 cagagaatca aaattaaaaa cagcgtggtc gaaattcgaa tacgattaaa catcaacgct
     1741 ccccatcagg cggcaatgac cgcgttagta aaaactcgtc aaaatgtttc ccagtttgga
     1801 tcttgttcag taattcgcag tcgtggctgt gccggtggtt gctcactggc aggacgggat
     1861 ggtgtttgcg gtttattggt gagtgggctg gctgccagac ggaacgcgga aaccgcttgc
     1921 gttaaacgac tcgcctgttc ttccagcgca gctgcggcgg cagctgattc ctgcaccagc
     1981 gatgcgttct gttgcgtgac gcgatccatt tccgaaaccg ccaatgcgac ttgatcgatg
     2041 ccacggctct gttcatccga tgccgatgca atctcgccca taatgtcagt cacgcgagtg
     2101 acagcattga cgatattgtt cattgtttcc ccggcgcttt cgaccagcac cgaaccggta
     2161 tcaacgcgcg agacggagtc ttcaatgagg gctttgatct cttttgccgc ctgggcgctg
     2221 cgactggcaa gattacgcac ttcacccgcc accacggcaa aaccacggcc ctgttcaccc
     2281 gcacgcgcgg cttcaaccgc ggcattcagc gcgaggatat tagtctggaa ggcaataccg
     2341 tcgataacgc tgataatgtc ggcaattttc ttcgaactat cggcgatctc atgcatcgtt
     2401 ttcactacgc catccaccac tttgccgccg tgctgggcgg tgtcggaggc actttgcgcc
     2461 agttgcgagg cctggcgggc gttatcggcg ttttgcttca ctgtcgcggt gagctgctcc
     2521 atgctggcgg cagtttcttc cagcgcggat gcctgctgtt cagtacggga ggaaagatcg
     2581 gtgttgcccg ccgcaatttc acgggtaccg gcatagatgg catctgaacc ttcgcggaca
     2641 tgagtgacgg tgtcagtcaa agagcgttgc atatgtgaaa cgctctgcgc caggtcaccc
     2701 atttcactgc gcccgtcaat ggtcagggta ttcgccaggt taccaccggc gatttcgcga
     2761 atgtgagcaa taatttttgc cagcggagta agcaacatac ggcgaatgcc gtaccacgcc
     2821 accagcagaa tcaataccac caccagcgcg ataaccgcca gttgccactg ggcaaatcgg
     2881 taatcatctg cgttgtcagt gacgatatcg cgatacagtt tttcactgct gagggcgtac
     2941 tgagcaaacg cttcgcccat tgcattttgc attccctggg ttggctgagc gaaataagct
     3001 ccagtattgc cataatcaag ataatcaatc agttcagtta acgctgtgta atagttttta
     3061 tatttttcat caaaattacg gctggtagcg accatttcag gtaacggtgc catgcttttg
     3121 aattttttat aatgcgtcgc ggcctgggcc aatgttttcc tggcgctatc gagcaattca
     3181 actttggcgt tactttgttg attggaggaa tccatcatca tccgcaccgc tgaacgactc
     3241 aggttaatgc gcgtttgcag cattaaatcc caggttgacg tcagctcgcc ctgctgttcc
     3301 cgtaattgat tggaaaccac aaagctcttc tggctatggt gaagggaaga aaaaaacaaa
     3361 ctgccggaaa taagctgtaa cagtgcgaat acccccagca ccattaccaa cagcgtgact
     3421 acgcggatac ggttaatcat aaggcacctt cctgaaaaca agttgatctc gttatcggca
     3481 agaagggggg aaactttatt gctgatgcca cccgccgcga aattgaaata aaaaacccga
     3541 tgcgcagatc atcgggttca tttcaattga ggaaatcggg agaattacgc cacttctgac
     3601 gccgcgctat ctaacagcgc catctcttcg ctgttcagca gtttttcgat gttcaccaga
     3661 atcaacatac ggtcgcccag tgcgcccagt ccagtgagat attctgttga aagcgtcacg
     3721 gcaaactctg gtgccggacg aatttgctcc gccgtcaatg aaagcacgtc tgaaacgccg
     3781 tcaaccacga tgccgaccac ccgctgtccg agattcagga cgataactac cgtgttgtcg
     3841 ttatagtcca catccacctg gctgaactta attcgtaagt caacaatcgg cacaataacg
     3901 ccgcgcagat tcgtgacgcc tttgataaac gctggcgtgt tcgcaatccg tgttacctga
     3961 tcgtagccac ggatctcctg cactttcagg atatcaatac cgtactcttc atcaccaagg
     4021 gtaaatacca gaaattcctg gcctgacggc tcgctggcca gctttgttac attcgtcata
     4081 ccggtcatat tgttaccttt ttactcattc aggcggcggt gttcgccata cgttgttcgc
     4141 ggtttatcgc ctgcaaggcg gagacatcaa caatcagtgc cacgctgccg tcgccaagaa
     4201 tggtcgcagc agaaatgccg gggactttgc gatagttact ttcaaggttt ttaaccacaa
     4261 cctggtgttg accaattaat tgatccacca gcaaggcgta gcggcgaccg ccactttgta
     4321 agatcaccac aattccctgg gtggcttcgg ttttcgcgcc cgcgacgttg aacactttcc
     4381 acagttcgac gatgggcaga tattcacccc gcacttccag cacccgctcg ccgccggcca
     4441 gtggatggag atcggcttca cggggttgca gtgattccat aacagcattc agcggcagaa
     4501 tgaaaacttc atccgcaacg cgtacggaca tgccgtcgag gatggccagc gtcagcggca
     4561 gtaaaatgcg gatcgtagtg ccagtaccct gcttcgactg gatttcgaca tgaccgccca
     4621 tctcctggat attacgttta acgacgtcca tgccgacgcc gcgcccggag acgtcggtga
     4681 cctgctctgc cgtggagaag cctagtgcaa atatcagcat tgcaacttcg tcgtcgctca
     4741 tgttttcgct gacagtcaaa ccttgcgagg ccgcttttgc cagaattcgc tcacggttta
     4801 gccccgcccc atcgtcggtc acttcaatgc aaatgttgcc gccctgatgt tcggtagaca
     4861 gaattaaatt tccgacgctg tttttacctg cggcgagccg tttttctggc agttcaatac
     4921 cgtgatcgag gctattgcgt accaggtggg tcagcgggtc gataatgcgt tctatcaggc
     4981 tcttgtcgag ctcggtggaa ctgcccacca gcgtcagttc tacctgcttg ccgagttttc
     5041 ccgccagatc acgcaccagc cggggatagc gactaaagac atattccatc ggcatcatac
     5101 gaatcgacat cactgattcc tgcaaatcac gggcgttacg ttgtaactgc cccatgctgg
     5161 taatcaaatc accatgatta accgggtcca gttcgctgga acgctgggca agcatggact
     5221 gggtgataac cagctcgccg acgaggttaa ttaattgatc aaccttttct accgctacac
     5281 ggatgctggt ggattcactg ctgcgcgtcg ttttttcccg ctccacgcgg ccggttggcg
     5341 cttgttcggc tgccagttta agcacaggtg gggtagctat ttttggcgaa acttctactg
     5401 tttcaaaggt aatctgatcg gcttcaatca caaaacagag taccgctgtg atgtcatctt
     5461 cggcgatatc gcccggtaat attgccgaga gagaatccgc ccctttcacc acgtcattta
     5521 acgttgtcag atgccccagc tcttcttcca gcaggtcgac ttcgccggcc ttcaggcgcg
     5581 aaaggataat tcgtcgcggc aactgactgc gactttgctc atcttgcggt tcacttttgg
     5641 caaccacact taatcgggtc actgcggatg gcgtttcgcc tttcgcttct aatgccagtt
     5701 gacgcaaggc ctggcagata taatcgtagc tggcggcatc cggctcttgc gactgtttat
     5761 aagcgtcgag ctgttcttgc atgatgtcct tcgtttccaa aaacagattg ataatgtcgg
     5821 tgttgagttg catctcacct cgtctggctt catcgagcag gttttccatc agatgcgtgg
     5881 tttcctgcaa aacgctgaag ccaaaagttc ctgcccctcc tttgatcgag tgggcagccc
     5941 gaaagatggc attcaattgt tcggcatctg gcgcttccgg ctgcaaaacc agcagatgct
     6001 gctccatatc agccaacagt tcgtccgctt catcaaaaaa tgtctgataa aaatcgctta
     6061 tatcgatgct cacgctgtca cctcggttcg gctgatggca ttgtgggaac tctgacctgt
     6121 ggtgcaacct caggtttttc cagggcgctt actggctcat tctggctttc ggcgttttca
     6181 tgcaaaatgg cctgttcggc ttgtttgttc agtaccagca ggctgatgcg acggttgacg
     6241 gcatcatcag gtccgcgatc gcttaagcgc atcgttgccg ccatgccgac gacacgtaac
     6301 actttgccgc tatccaaccc tccgaccatc agttcgcggc gggatgcatt ggcccgatcg
     6361 gcagaaagct cccagttgct gtaacctttc tcaccgctgg cgtagggaaa atcatcggta
     6421 tgacctgaaa ggctaatacg gttgggaata ccgttcagca caggcgcgat ggcgcgcaga
     6481 atgtcgcgca tatagggttc gacatcggca ctgccggttc taaacatcgg gcgattctgg
     6541 ctatcgatga tctgaatacg tagaccttcc tggaccagat cgattttgag atggagacgt
     6601 aacgcccgca gttccggatc ggactctatc aactggtcga gatcaccccg caatttccgc
     6661 aatcgacttt gctccatgcg ttttttcagc tcttcgatgt tcggctgctt attcacttcc
     6721 ccctggcttt gggtgtaatc atcaccaccg ccgggaattg ggctttcact attggaaatg
     6781 cgatcgccgc ccgtaaccgc tgtcgccagt ggagtccgga agtactccgc aatctgaatc
     6841 aactcttttg ggctggagat ggagatcagc cacatcacca gaaaaaaggc catcatcgca
     6901 gtcataaagt cggcataagc aatcttccac gatccatgtg ctgccccgtg gcttttggct
     6961 ttgcgtcgtt tgacgacaat aatcggatgc gcttgattct tcatgcttcc tcggttgtcg
     7021 tctgttattg cggatttttc accgcacgca catgctcttc cagttcaata aacgacggac
     7081 gttcgctgga atagagcgtt ttgcgaccaa actcaacggc gataggcggt gcgtaaccgt
     7141 tcagattaga aagcagagtg actttgacgc actgcatcat tttgctggtt tcgccgcttt
     7201 tctgacgtaa aacactcgct agtggggaaa taaatccgta agccaataaa ataccgagga
     7261 aagtccccac catcgcatgt gcgataagcg cccccagctc ggcggcagga cgatcggctg
     7321 aacctaacgc gtgaacgccc cccattacag ccgcgacaat accaaacgcc ggaagtgagt
     7381 ccccgaccag cgccagactg ttcgccggga cttctgcctc gctttcgtgc gtctcaatct
     7441 cttcatccat cagggcttcg atttcgaagg tgttcatgtg accgctgata atcaggcgca
     7501 gataatcgac gataaaatca agcatgacgc tatccgcgag gatgcgtggg tagctggcga
     7561 agatctcgct ctcacgggga ttttcaatat cacgttccag cgaaaacatc cccatctgcc
     7621 gcgatttcgc catcaaccga taaagcagag ccagcagatc catatacatt gctttggtgt
     7681 atttggagcg acgaaacagc aacggcagcg ctttcagcgt gcctttaatc gctttgccat
     7741 tattgccgac gataaacgac ccaatccctg caccggcaat aatcaccagt tcagcgggtt
     7801 gatagagtgc tccaaggctt ccaccggtca tcaaataacc gccgaaaact gtaccgagaa
     7861 caaccaggta acctaataag ataagcacga catcatcctt ccactgttga ccatgacagg
     7921 atgttcagtc gtcaggcgtt aacgcgcgat tggggcaaaa aaaagcagcg gtacgtcgtt
     7981 accgctgctg gaatgttgcg ccacaccgta tcagttaaac agcctgtact ctctgttcat
     8041 ccagcagttg tgggataata tcggcaggat tctgggaaag tttacgtctt tttactgccc
     8101 gggatggcgg ttgacataag ctgcaggcaa agctgccaac aggctggtga gcgtgggtaa
     8161 taaaattgcc gccgcagcag ttgcagctgg aaagttgcag taatccactt tcaacaaacc
     8221 gcaccaatgt ccaggcacgg gttaatgcca gcagtggtcc ttcttctgct tgtgggcact
     8281 gttcaaggta taaacggtag gctttgatca ccgcatcgac gccattacac aaaccggttt
     8341 tcagtaaaaa ctgccatgca ttacagaaca tcgaagcatg aacgttttgt tcccaggtca
     8401 taaaccagtc ggttgaaaat ggcagcatgc ctttcggcgg tgggcttccg cgcagttctt
     8461 tataaagttt tatcaggcgt ccgcgactta actgtgtttc gctttccagc atctgcaaac
     8521 gagcgcccag ggtgatcaat tccattgcca gctgaatatc ccgcgcttcc tgaacaatgc
     8581 ttttttcact catgatcagg cccttttctt gcgcagcgct tcttcaggct gattaacatc
     8641 attcagcaag cgtgttgaga gcatgatgcc ggtatgaatt tgctggagat cgtcaacgcg
     8701 ggaatcttgc gtcaactgag taatcgtctg gtggctgtca aaacggaagt gacaaaccag
     8761 ctgattggtt tctgccagct taaccatttg cggaagagtc agtgccgcta acgttgtcgc
     8821 catttcttca tttatgccga gacgaaacat agcggacgct ttgtcctaaa caatcagacg
     8881 ctgtgcaagt agtaaatatg acaagttgat gtcataaatg tgtttcagca actcggaggt
     8941 atgcattatt cccacccaga ataa
//
LOCUS       DADLSA010000152         8943 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.153, whole genome shotgun sequence.
ACCESSION   DADLSA010000152 DADLSA010000000
VERSION     DADLSA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8943
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.153"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(123..1040)
                     /locus_tag="JN691_003441"
     CDS             complement(123..1040)
                     /locus_tag="JN691_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAY7244790.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVFMTR"
     gene            1100..1378
                     /locus_tag="JN691_003442"
     CDS             1100..1378
                     /locus_tag="JN691_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244791.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1379..1906)
                     /gene="gadE"
                     /locus_tag="JN691_003443"
     CDS             complement(1379..1906)
                     /gene="gadE"
                     /locus_tag="JN691_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAY7244792.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNILEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2705..3277)
                     /gene="hdeD"
                     /locus_tag="JN691_003444"
     CDS             complement(2705..3277)
                     /gene="hdeD"
                     /locus_tag="JN691_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAY7244793.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3532..3864
                     /gene="hdeA"
                     /locus_tag="JN691_003445"
     CDS             3532..3864
                     /gene="hdeA"
                     /locus_tag="JN691_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAY7244794.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3980..4306
                     /gene="hdeB"
                     /locus_tag="JN691_003446"
     CDS             3980..4306
                     /gene="hdeB"
                     /locus_tag="JN691_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAY7244795.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4370..5016
                     /locus_tag="JN691_003447"
                     /pseudo
     CDS             4370..5016
                     /locus_tag="JN691_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(5058..5588)
                     /locus_tag="JN691_003448"
     CDS             complement(5058..5588)
                     /locus_tag="JN691_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7244796.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNI
                     KRINELVRHQHIDYLV"
     gene            complement(5744..6310)
                     /gene="slp"
                     /locus_tag="JN691_003449"
     CDS             complement(5744..6310)
                     /gene="slp"
                     /locus_tag="JN691_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAY7244797.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6557..7780)
                     /locus_tag="JN691_003450"
     CDS             complement(6557..7780)
                     /locus_tag="JN691_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244798.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSENKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8361..>8943)
                     /locus_tag="JN691_003451"
     CDS             complement(8361..>8943)
                     /locus_tag="JN691_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7244799.1"
                     /translation="FERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIEN
                     PVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHA
                     GADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVI
                     LLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2597 a         1756 c         1853 g         2737 t
ORIGIN      
        1 ttatcgatat cgaagcattg agcattttta actcgtttta tcttgaatgc aatcggcgca
       61 taacttattg aatcccatca aatcccgtct ttttttaccc attcccggtt atcttgaata
      121 aattacctag tcataaatac attctggcgg ctaccttcat ccactaatgc cgtgacatac
      181 atgccaggca gcaagtcacc atttgggttg gggaaaatcg cccgtaacgt cacggagccc
      241 gtggtttcat caaccgtcgg gtcggagaat ttcagcgtgc cggtctggct gtaacgttta
      301 ccattttcca gattgagctg tactggcgta ctgccctgaa cctgtttgat ttgcccactg
      361 gcgacctctt ctttcatgcg taagaaatct tgcaccgact gcgtgagatc gacataaatc
      421 gggtccagac gttgtacggt aaccagcgaa tctgcctgat tagcggtaac gagtgcgccg
      481 acggtcaccg acgatttccc gctgacgccc gtaatcggcg aggtgacatt cgcgtattgc
      541 agattgatcg tcgcctgttc aacagccgct ttggcgacgg tgacattggc ttctgcttca
      601 ttcagctggg tgcgcgcggt gtcgtaatcc tgacggctaa cgtagttggt cttcagcaac
      661 gatgcctggc ggttaaaggt gatgcgggca ttgctggcgg tagagagcgc tttcgccagc
      721 gagcctttgg cggagtttag ctcggcctgt aaaggtgcag gatcaatctg atacagcgaa
      781 tcgccctggt tcactttatc gccttcgata aagttgcgtt taatgataat accgcccacc
      841 tggggacgta tctcggcaac ttcataagga acggttctac cgggcaattc gctcaacaca
      901 ttgaccgaac caggggagag tgtgacgaca ccgacctcag gcgtcatggc ggcggcgttt
      961 tccgccgatt tgtcatcgca ggcggtgagc atcgcgccgc agaataacaa cggtattaac
     1021 agctttcttc ttctgttcat tttagtccct gaaaattctt gagaaataac ggttctttaa
     1081 attgcgcgcg aggagagata tgagggattt acagaccagc ggcatagtgg ggttgtcagc
     1141 cagtaaggtg ggatacaggc acagtgatcg acatggtgag gtcaacgaca aaaaaaatgt
     1201 tgccgttctg ccaacagttc ctgccagcat tcgggcaacg aagggttcta ctggtggata
     1261 cacataccag gggaatcaag tcgttttagc ctctccttta gtaatcaccg ggggtaatga
     1321 aatcagcatc tgcatccctc gtcatgccag ccatcaattt cagttgctta tgtcctgact
     1381 aaaaataaga tgtgataccc agggtgacga tgtcgctcat acgtcttagt ttcagcttgt
     1441 acataatgtt gaatttctga atctttaatg tttgttgcga aatattgaga gtcgacgtga
     1501 tattgctttc atttttattt ttcagaatca gttgcataat tttgaactga ttatgtgaaa
     1561 aacataattg tgaattctta tggggcaagt gtttaccata agtaatctca agaatatttt
     1621 tgatactttc tttgcggcta acaaaataaa taggcgcttt agcttttagt ttactgatgt
     1681 gataaggtgc caaaacaata attctctcgg catctaattt ctccagaaat ttaatcgctt
     1741 cttcatcaag gatatgattg tgataggtat caatgataac cttgaagggt ttattgccta
     1801 cttttttgat ctctgacagg gaattgattt ttatcatttc gtgattatct ttcaactgcc
     1861 aaaagccctg taaaagaaaa gaatctttcg tcatgagaaa aatcataact tgctccttag
     1921 ccgttatcgt ttcgaacatg tcatcctttt cattatttac atccttgtcc gaatcgttgt
     1981 tcaatatagt aaacgcctat aagaactttg cccttcgtga actaatacgt ctattaacac
     2041 taatcaatac ttcagattta tccatcacgc actccatata ttgatcctaa gtaagattcc
     2101 tggttgttat cagcttgtaa atagtaaaca aaaagttaac gtttgcggat tactgcccaa
     2161 gaataagtta tcaataacca gtttgtgatc tctgaagaat attactaaag ttaaaatgta
     2221 atccgattta aatatcgagt ctccttgttt cgacttaagc tggcaattgg attgccagct
     2281 ttcttttaac gcattgaaat tacctgagta aataacgcat tcatgtcttc aggtttttat
     2341 atagcgtctg gagacacgga tatttatgca atgtaatcaa cgccagttgc aaaaaaatga
     2401 acccggatta cttatttaac cgcccatagc atcaacgctt acggaactct ttgttcactt
     2461 tcccacagca cttttaaaac attaaacctg actacggaaa atatcagcca tgataatgtt
     2521 tgaaatggct aatttgccat ggagtgaaaa aaattagatg aaattcagta ggttgaaata
     2581 atcactagca ggtaattatt tcaatgatag tgcgcaattg atctacaaca ctgcgtagcg
     2641 gagagagtat taatcggatc atagtcacat caagtgacta tgatccgggt gacaaccggg
     2701 gtaattattg ctgcttaacg aacaaactgg cgaagctgaa caggctggcg gcgctaaata
     2761 tcagttcaat tcccaccagt gtggaaacca gcgttacaga caccatcggc gttgcaccaa
     2821 ggaatatcca ggcaatgacg atatccagca caccaataac gagctgtagc cagctgcctt
     2881 tcattgaacg ctgacgatac caactcatca ggcgaataac ccctgcaaca cagaacaaac
     2941 cggcaataaa tgccgcaatg gcaaaaatac ccagctccgg tgcgcggatg aagaaatagc
     3001 cgatcaataa ataggcgact gcgacgagga aaccggataa taccggccag aaattatgac
     3061 tgcggttgct gaataacccg acaataagcg caatacccga gcagattaat aatgcaccca
     3121 ctactgtgct taaaatatcg ccagagacga acgggaaact gatacacagc aacccgacga
     3181 taaacagcag cacggcaata aactggattg ctctgcgatg ctttttcagc atctccagat
     3241 caaacttcaa aattgttgcc ttatctatat ataacataga accaccctat aaaattaaga
     3301 agaaaatccc ctgctatcaa tctatgccaa aaacgcgtct aagaatacag tcgatttaat
     3361 aaaaatttcc taattgcagt atctgatgca tctgtaactc attgtattga aataaaaata
     3421 tctgattttg atattttcca tcaacatgac atatacagaa aaccaggtta taacctcagt
     3481 gtcgaaattg attcgtgacg gctctttcac tttatagttg aggatattac gatgaaaaaa
     3541 gtattaggcg ttattcttgg tggtctgctt cttctgccag ttgtgagcaa tgcagcggat
     3601 gcgcaaaaag cagctgataa caaaaaaccg gtcaactcat ggacctgtga agatttcctg
     3661 gctgtggacg aatccttcca gccaactgca gttggttttg ctgaagcgct gaacaacaaa
     3721 gataaaccag aagatgcggt tttagatgtt cagggtattg caaccgtaac cccagctatc
     3781 gttcaggctt gtactcagga taaacaagcc aactttaaag ataaagttaa aggcgaatgg
     3841 gacaaaatta agaaagatat gtaattccgg gaatgcgtta catcgtactt ccttgcatat
     3901 tgaacaggcc ggaatatctt ctttaaaagc agctattcct cctgttcata tataatctct
     3961 atattgaatg ggtcacaaaa tgaatatttc atctctccgt aaagcgttta tttttatggg
     4021 cgctgtagcg gctttgtcac tggtgaacgc acaatctgcg ctggcagcca atgaatccgc
     4081 taaagatatg acctgccagg aatttattga tctgaatcca aaagcaatga ccccggttgc
     4141 atggtggatg ctgcatgaag aaacagtata taaaggtggc gataccgtta ctttaaatga
     4201 aaccgatctc actcaaattc ctaaagtgat cgaatactgt aagaaaaacc cgcagaaaaa
     4261 tttgtatacc ttcaaaaatc aagcatctaa tgacttgccg aattaatgag gtacaagtaa
     4321 aaaggagtag caagttgagc catcttgctg ctcctttttg catttttata tgacagcaga
     4381 atttattata cgtcttatac tcgcggcaat tgcctgtggc gctattggca tggaaaggca
     4441 aatgcgcggc aaaggagcag ggttacgcac acatgtatta attggcatgg gaagcgccct
     4501 gtttatgatt gtttcgaaat atggttttgc tgacgtgctg tctttagatc acgtcggact
     4561 cgaccccagc cgtattgctg cttaggtggt gacgggcgtc gggtttatcg gtgcaggtaa
     4621 cattctcgtt cgtaaccaaa atattgtagg tctgacgacg gcagcggata tctgggtgac
     4681 cgccgcatag gtatggttat tggcagcggt atgtacgaac tgggtattta tggttcagtg
     4741 atgaccttgt tggtgctgga agtcttccat caattaacct tccgcctgat gaacaaaaat
     4801 taccatttac agctgacatt agtgaacggt aatacggtat caatgctcga ctggtttaag
     4861 cagcaaaaaa taaaaactga tttagtttcg ttgcaggaaa atgaagatca tgaagtggtc
     4921 gccattgata tccagcttca tgccacaacg ttgatagaag acctgttgcg attattaaaa
     4981 ggtatggcag gggtgaaagg cgtttcaatt agctaatctg tgtggcagcg tagccagact
     5041 caccgtaagc ctgaaattca caccagataa tcaatatgct gatggcgtac cagctcattg
     5101 atccgcttaa tattgaggat catcatgata ttgtgtttat ggcagtagaa cgtttttagc
     5161 gatattttca gtttctccag aatatcttca gttgtcattt gattcgcaat gcagctcaga
     5221 acctgatgtt gcttgcgcga aagtgacaca ttcgcttcag gcacagagca gtaactgcca
     5281 ttgatgagtg cgacaaaatc aatgatagtg atagtccgcg gaacaaaggt caccggtact
     5341 ttccccagac agcgtttaat attaaagggg gagaaaacaa tgacatggac aggttttaat
     5401 cgttcaattt gagtgagaaa attagaatga aatacattat tcattaatgt atcaataatg
     5461 acgaaggcat tctgatgcaa cattacgtcg atctcttctt cgttctgtat atgaacgaca
     5521 ttacctttac tcagaatgtt tttcatcgcg gtgaagaaca tcgtatccct ggtaattata
     5581 agaaacataa ccgactcctg gtgcggacat taactttata atcagtttgc caaaggtaat
     5641 taacaggaca gggcttataa taataatagt tagcttactg tatattaatt ttatgtaata
     5701 catgatgcag atgctgagaa agcatctgca tctttcggtg gtgttatttg accagctcag
     5761 gtgttacctg actcaccgca ttggtgtagt aaggcgcacc ccagcccggt tccggccaga
     5821 atgcaccata gccgtaatcc cacaggttat aggtggtatt aaccacttct ctcaaatgcc
     5881 acacctggat gccctgcata ttcacttcta ggaagttata cgggacttta ttgataaagc
     5941 ctggttgttc accctgaatg gtaccgagga tggtaacaaa gtgattacga tagttcactg
     6001 gatcaaggaa gccgctttgt ctggcgagca gtcggccctg atagttggct tcaatatcag
     6061 gcttcgcata gctatccaac ggtaatacag cgatttctaa caacgtatcc gttttgccat
     6121 tgataacgtt gataaccttc ccaccaaagc gcgcttgttg accaacatat aaccccggct
     6181 ggttatgaac agcaacaaaa cttttttgaa tatcaggttg gttattgcct ttgatatttt
     6241 gcggaattga actacatgcg gcaagcaaaa atgaaaggct gaggatgagt gcaccttttg
     6301 tcatgttcat gttactatcc ttatcaacaa atattaaaac cataatagat atttctttat
     6361 agttttcatc tgattctgag gtgatatgcc tgatagtaac gtaaacctgt ctgatgttta
     6421 tatcccttct gtttagttca attccgttat tttggggcgt ggtatgagta tccatcgccc
     6481 aataatataa ccctttgttt taaaattaca taaaatctat ttttattcaa tgattataaa
     6541 gtgctaataa tcaatattat ggtattatat tctgataatg atttgataag cgaattcttt
     6601 tgccagatgg tatttctatt tccaccccat aatttatatc tttttcatta ggcagatctc
     6661 tctttaaata aaaggggtat gaaatctttt cgggttgaag tgtctcgtaa tgaaatgcta
     6721 tgaattcatt tttagacatt gttgcccatt tttgaatttt attgtataga gagggtgttg
     6781 taaactcttg aagttgttta taacatattt ttttgaactc ttgcttatat tttaatatgt
     6841 catcaaatga aagattcact acctttgcct ctaagaaatc aattattctc gaagcaattt
     6901 ccgctttcgc gcgtaaagag ttgtcattaa tagttaacaa tgtagaaaac aagccattga
     6961 tttgtttgtc aagatgctgt tcatatttat gtagtttggg gatgggattt tccttgtgtg
     7021 aaaaaatagt gttaaataaa tttgcgtcag tgaaatttat attaagtaga tcttcaaaat
     7081 aaaattttga taaggttagg actagcttgg acatatcttg ccctgtcatg ttgaaatcaa
     7141 taaagtcgca tccagcaagg gcttccatcc tattttcatt tttactgtgc tcaatataca
     7201 gcttcgccat ttttaagtac aagctttgaa tgtctcttat atcagttggt aagtcagctc
     7261 gttgcccagc aaaattttta ttttcggaag aaacggtttg gacactgaat ctttcgggta
     7321 ttccttcttc tacgagaatt ttagttatga ttggattgtt gggttctgga ttaacgacaa
     7381 caatgatttg acatacagaa tccttatcct tttcgtatct aatgttatga atctgattaa
     7441 gtattgattg tgtctgttgc gtaagtggtg tcgaacttat cgtttcaatg ttattaataa
     7501 gacttacaag ttgaaccaca acttttgagt agtctgcatt tgtatatgtg gtgttcgtgt
     7561 ggcatgcatt aatttgttgg agttgttgtt gcgctaaatc aaattgttca gggttgtatc
     7621 cttctccaga gaatgtggaa ttaatgggtt gttgttcttc ccaattatca caaggccact
     7681 tcggtgtgtc tggcgtttta acattatcgg tatttgttgt gcagttataa atatcgccgt
     7741 gatatgtatt tattatacct ggggtaaagt caattgacat aacattcctt attcatcatc
     7801 tgttttatgc taatgttttt aaattggata attagaacag gaatggattt tcttctacac
     7861 acaataaata taatgcgtta tatcttcctg atataattac ctttaaattc cctttcattt
     7921 ccacccggta atatttgtgt ataaaaaaag ggcagtgata gtatcgtaaa ggaaaacgtt
     7981 aacaactatc aaaaagcgct cattttaaaa agttattatg ttctccgata gtgtgtgaag
     8041 taatattttt gtgatgtttt agatgtgtaa taatgaacta ggttaaagtg tatttaaaaa
     8101 tgtgttgtat tagcgactga aatctgcctt ttaaaaagga ggagggtact gagacaaaga
     8161 gtcattgctt ctccgataaa atgtcagaga agcatgatta cgtggttatt gtctgttgat
     8221 gacttgttga ccgagccgtc atcattctca atgcattagt taaatcattt gtgattttta
     8281 aggattatcc tgtctatgat tttctactta gaaggatatg gtactaacta tgcatcttat
     8341 agtttactgc tggcatctat ttaaaatagc aattgagtca aatagcccat caagatagcc
     8401 atacctaata taaccgtcag gaatgcagct atcatcggca ttctgaacat tgatttaagt
     8461 aatatcactt ccgtcaggct tgcgccggca ctgccgatga ttaatgccat taatgctccc
     8521 agacccattc cttttgtcat cagaacagat gccagaggga taaccgcctc agcccggata
     8581 tacagcggaa taccgacaac ggcgcttaat gggatggcga agggattatc tgctcctgca
     8641 tgagcggcaa tccacgctga gggaataaag ccataaataa aagatcctat taatactcct
     8701 aacaaaagat atggcagtac atctttaaac tgttgcaacg catctttggt ggctagtttt
     8761 atgggattaa tacttactac agctttggta ctgcaacagg taactacggg gttctcaatg
     8821 gctttagccg ctggactaca acaactcact tttatgggcg tatatgtcgt tcccggcgaa
     8881 gtttttgctg gagcacaaca atttgctgat gagcttttac tggcaatgat atgacgttca
     8941 aac
//
LOCUS       DADLSA010000153         8840 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.154, whole genome shotgun sequence.
ACCESSION   DADLSA010000153 DADLSA010000000
VERSION     DADLSA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.154"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(160..1131)
                     /locus_tag="JN691_003452"
     CDS             complement(160..1131)
                     /locus_tag="JN691_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HAY7244800.1"
                     /translation="MKRAPVIPRHTTHSQSTEDTSSPAPAAPMVDSLIARVGAMARGN
                     AISLPVCGREVKFTLEVLRGDSVESASRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSDVIEIADGSRRRKAAILTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGLRYTSRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFAHPGELSA
                     RSGEALQKAFADKEELLKQQAETLHDQKKAGLIFEAEEVISLLTSVLKQSPASRVNLS
                     SRHQFAPGATALYKGDKMVLNLDRSRIPAECIEKIEAILKELEKPGV"
     gene            complement(1131..2297)
                     /gene="sopA"
                     /locus_tag="JN691_003453"
     CDS             complement(1131..2297)
                     /gene="sopA"
                     /locus_tag="JN691_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HAY7244801.1"
                     /translation="MGLMDTLNQCISAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMETRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DAFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDASYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPA
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNNNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            3038..4048
                     /locus_tag="JN691_003454"
     CDS             3038..4048
                     /locus_tag="JN691_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7244802.1"
                     /translation="MTSENNSLLLNLQEVDKTTGEVVKLDVNSTSTVQPVALMRLGLF
                     VPTLKSTGKSKANRKNVTDATEELVQLAIAKSEGYTDVKITGSRLDMDTDFKVWLGII
                     RSMSEYGVKSDTLELSFVEFVKMCGFDSRRSNKKMRDRISNSLFKLASVTLKFQSETK
                     GWTTHLVQSAYYDINEDIVEIKAEPKLFELYHMDRRVLLRLKAIDALQRKESAQALYT
                     YIESLPQNPAPISMKRMRDRLNLTSNVYTQNHTVRKAMEQLRDIGYLDYTEFKRGRAT
                     YFSVHYRNPKLISSPVKVPRKEEEEKAPEQNYDEVIKALKAAGIDPLKLAEALSAMKP
                     EN"
     gene            complement(4854..5183)
                     /locus_tag="JN691_003455"
                     /pseudo
     CDS             complement(4854..5183)
                     /locus_tag="JN691_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5301..6026
                     /locus_tag="JN691_003456"
     CDS             5301..6026
                     /locus_tag="JN691_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY7244803.1"
                     /translation="MAYSIGEFARLSGITATTLRAWQRRYGLLKPQRTEGGHRQYSDE
                     DVQQALKILDWVKKGVPIGQVKPLLERPTSRLTNNWLTLQQSMLQRLQDGKIESLRQM
                     IYDAGREYPRPELVANVLRPLRSQVSANVAAAMTLREILDGIIIAYTSFCLEGDKKAP
                     GDNILLSGWNLNDPCEVWLEALTRTGQGHRIDILPTPPATLAPEIFPDRHWLLVTRGK
                     LTAARKKQIEQWQQQVSLEVIIL"
     gene            6126..6536
                     /gene="uraH"
                     /locus_tag="JN691_003457"
     CDS             6126..6536
                     /gene="uraH"
                     /locus_tag="JN691_003457"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY7244804.1"
                     /translation="MKKTASLLMLTTLAFAPAAFSAPAGTLSVHILDQQTGMPPSDVT
                     VTLEKQQQDKWTPIASGKTDHDGRIKSLYPEDQDMQPGVYKVAFKTADYFHGKKLDSF
                     FPEIPVLFTVTRTNEKLHIPLLLSQYGYSTYKGS"
     gene            6639..7598
                     /locus_tag="JN691_003458"
     CDS             6639..7598
                     /locus_tag="JN691_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HAY7244805.1"
                     /translation="MTTKPVLGISGCLTGSAVRFDGGHKRMGFVMDELAQWVSFRPVC
                     PEMAIGLPTPRPAIRLTLTDSGETQLRFSKPPHDDITQKMADFTADYLPKIGDLSGFI
                     VCAKSPSCGMERVRLYDENGNRGRKEGVGLFTAALLETYPWLPVEEDGRLHDPVLREN
                     FIERVFALHELNTLRAKGLTRRALLDFHSRYKLQLLAHHQAGYREIGPFVASLHEWED
                     LDAFFVAYREKLMTILKKPASRKNHTNVLMHIQGYFRNQLNSRQRVELRDVILHYRDG
                     LLPILAPLTLLKHYMAEYPDRYLMTQNYFDPYPDDLGLRLAVT"
     gene            7744..8832
                     /locus_tag="JN691_003459"
     CDS             7744..8832
                     /locus_tag="JN691_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7244806.1"
                     /translation="MITTLHIQNYRSIREMSLELEQLNIVFGPNGTGKSNIYKAIHLM
                     HSAAQGQFSQALANEGGILKVFWAGKTRSDQLRRMNLAVETETYEYELQVGFVEKLPY
                     PSQFQLDPVIKEESIWLSGQYRRPSSQLMKRKNQAVFLNNVHHEKVTHSGTLYENESV
                     FGQLGEPHLYPEVSQMRESLRNWRFYQEFSVSIGSAMRAPQVGFRSPVLASDGANLAA
                     AFQTIVEIGDELLLMRILDQAFPGCVFYSDNTGGRFRMMMQREGLSRPLEPAEFSDGT
                     LRFLCLAVALLSPRPPAFIALNEPENSLHPQMLPALASLIAEASRYSQIWLTSHSPEL
                     ANLIEKQRSFSLYQLSMVEGETKVERLG"
BASE COUNT         2235 a         2336 c         2255 g         2014 t
ORIGIN      
        1 gacgacgcag gtgggaccgt ggtcccaggg agataacctg agcggcgata caggtgggac
       61 cgtggtccca gagagaatat tcaggccagt tatgttttct ggcctgtgag aaaggaaatg
      121 aagtaaaggg agatcaccgt agactaaagc gtgtccgcat cagactcctg gtttctcaag
      181 ctccttaaga atggcttcta ttttctctat acactccgca ggaatgcggg acctatccag
      241 gttaagcacc attttatcgc ccttatacaa tgctgttgcc cctggagcaa actgatgacg
      301 tgagctcaga ttaactcttg atgcgggaga ttgtttcagt acggatgtta aaagactaat
      361 gacctcttca gcttcaaaga ttagtcccgc tttcttctga tcgtggagag tctcagcctg
      421 ctgcttcagt aattcttctt tatctgcaaa agccttctga agggcttcac ctgaccgggc
      481 agacagttct ccaggatgtg caaacagggc aacaacggat ttaggcaatt tggccgtatt
      541 gatacagcgg gtaatgatct tacgagagat gttttccgca tcggcaagcg cagaaatatt
      601 tcctgcaaac tcattctgca gccggcttgt ataacgcagg ccacgttcat aagcactcgt
      661 cggccgataa tcgttaccca gtcgggacaa tgcagccatc tgctcatcgt ccagttcacc
      721 aaccagaacg cgataatcac tttccgtcag gattgcggct ttacggcgac ggctgccgtc
      781 ggcaatttct atgacatcag aaacccggcg gccgaaagct ggagtctgct gaccggtcag
      841 taaaaatgaa ggaatgagat catccagagc atcttcggta agtagttcct gatcgcgctc
      901 attgcctgac catacgcgtg aagcgctctc aacagagtct ccccggagca cctcaagggt
      961 aaatttcact tcccgtccac ataccggaag agagattgca ttaccgcggg ccatagcacc
     1021 cacgcgcgca attaatgaat ccaccatcgg cgcggccggc gccggtgatg aagtatcttc
     1081 agtcgattga ctatgtgtgg tatgtcttgg aatgacagga gcacgcttca ttatctaatc
     1141 tcccagcgtg gtttaatcag acgatcaaaa atctcattgc aaacaggctc ccagatagag
     1201 agggcattac gccaggcacc ggttgaagaa cgttgatcaa tagcctgttc aaaaacggtt
     1261 ctcatacgga tctgaccttt accgacttca tccgtttcgc gcacaacatt tttaaggacc
     1321 atgcttcccc aggcatcccg aatttgttct tccatccacg gggactgtga gccattatta
     1381 ttactgtatt tggtaagcag aatacggaca tctggctcga agcctttaag atccacgttc
     1441 ttaagcaggt cacgaagcat atcgaaaaac tgcagtgcgg aggtgtagtc gaacagctct
     1501 gccggagtag ggacaatcag aacatcagca gcgcatacaa catttatcgt accgatacca
     1561 aggttgggtg cactgtcgat aactatcacg tcataatcat gagcgacagt ttcaatggcc
     1621 agacggagca tcaggtgcgg gtcagcaggt aatttgcctt cgtcaaattt ccccatcagc
     1681 tcggtttcaa tgcggtgcag tgccagacag gacgggatga tatcaaggcc aggccagcag
     1741 gtgggcttta tagcgtagct ggcgtcatcc ttttccccaa gataaaaggg caggagagtg
     1801 tcctcagcat gaatatgaag atcgggcacc catccgtgat acatagaagc agttccctgt
     1861 gggtcattgc cttcaacaag cagaacacgg agtcctttca gagcaagatc ctgagccagg
     1921 tgaacggata ctgaggtttt gtaaacacca cctttgtgag ctgcaacgcc aatcaccggc
     1981 ggaaacgcat cttcagcacg gcgcagtcgt gtaccaaata catcccgcat gtgattgatt
     2041 tgttcgatgg tataaccaac gcgttgctca actctcccac gggtttccat atcagggtgc
     2101 ggcagccggc cagctttctc ggcatccctg atagcctgag atgacacgcc aactaaatct
     2161 gccgcttcac caatcctcca gcgccgggtt attttccggg cttccggact gtcatcatta
     2221 aactgtgcaa tggcgattgc tttcgtcatt tcatgaccag cacttatgca ctggttgagt
     2281 gtatccatga gtcccatact gaacatcctt taatcatcac tttgcattat tttattaaat
     2341 cttggaattt attgcaaagc aacaataaaa tcgcaaagca cacgaaaaag tgcaaagtga
     2401 accacattgg gtaatgaaag aacgcagggg attacaaaag agtacatacc gcagtcaata
     2461 ttaatcactg ccgctcgccg cagtaagcga tctcacacag tgagaaagcg gcgaggaagc
     2521 ggaaaatcac agaactcaca gattgcacgt acgtcgggtt gctaaagatg actcgggatc
     2581 agccagttat aatgaaattg actgcggtat gtaatatatt gactatggta tgatgcttac
     2641 cgcatctgta ggaaagacga ctcagcatac cgcagtcaat gtttcattgt tgtacctgta
     2701 gatctcaatc ttccagagcg tattcaggaa aatcaggcca ggggtctgga tatctggtgt
     2761 ggcgcttccg gccttgtatc tgggttattc atggtctcga tccaataatt gatcccaagg
     2821 agatcatagt gagatccaaa agagatcccc ggatcgctcc agcatgcgct gtaactggct
     2881 ttaacgtctg tctggatcga ctgcggaatg taaaatgttg actgttatat gcataatcta
     2941 atactgtgat atgcaacggt atttactgag gtatgtatat ccgttgactg cgatacgtat
     3001 tactacatcc ttttgtggat aaccgttgag gatcaagatg acgagcgaaa ataatagctt
     3061 acttctgaac cttcaggaag ttgataagac aaccggtgaa gttgttaagc tggatgtcaa
     3121 cagcaccagt accgtgcagc cagtagcgct catgagactc ggtctcttcg tcccaactct
     3181 gaaatcaaca gggaagagca aggcgaaccg gaaaaacgtt acagacgcga ctgaggagct
     3241 tgtacagctg gccattgcca aaagcgaagg atacactgac gttaagatca ccggttcgcg
     3301 tcttgatatg gacacggatt ttaaagtctg gctggggata attcgctcca tgtcggagta
     3361 tggggtaaaa agtgacaccc tggaactgtc gttcgtcgaa ttcgttaaga tgtgcggatt
     3421 tgactcccgt cgttcaaaca aaaaaatgcg cgatcgcatc agtaattccc tgtttaaact
     3481 cgcctcggtt acgctgaagt tccagagcga gaccaaagga tggaccaccc atctggtgca
     3541 gtcagcctat tatgacatca acgaggatat cgttgagatc aaggctgaac ctaagctgtt
     3601 tgagctttac cacatggaca gaagggtact gctgcggctg aaagccattg acgccctgca
     3661 gcgcaaggaa tcagcccagg cgctttatac ctacatcgaa agtcttcccc agaatcctgc
     3721 acctatttcc atgaaacgga tgcgtgaccg gctgaatctc acctcaaatg tctatacaca
     3781 gaaccacacg gtgcgtaagg cgatggagca gctgcgtgat attggttatc tggattacac
     3841 cgagtttaaa cgtggccgag ccacctattt cagcgtgcat tatcgcaacc ccaaactcat
     3901 cagtagtcct gtgaaggttc cgcgcaaaga ggaagaagag aaggcgccag agcaaaatta
     3961 tgacgaagtc attaaggcgc tgaaggctgc tggcatcgat cctcttaaac tggctgaagc
     4021 cctctcagcg atgaagccag aaaattgact gcgatatgca tatcatgact gccgtatgcc
     4081 gctaaggaca ttccattgac tgtgatatgc atcacgcata ccgcagtcaa tatttattct
     4141 ctatcatgtc tgcccggatc gaacaatatt gactgtcata tgtgatttcg ggtcatgata
     4201 cgcagatatt gactacggaa tgcgggttta atgggggtaa tccctggtct gttcaccatt
     4261 ccatggggga taacatgcca cagtcaattt ttcagtaacg ctctatatcc cttacgaaaa
     4321 tctcaatcgg gtgctgagac ggtcatgtta cctgacggta tccgttcccc tgtgtactgc
     4381 aggtttagcc cggcaggatg agactgcagc tgtctgtata acccggcgcc agcttcacac
     4441 tgttaaatgc gatcaggaaa cgggagccga atggcagaaa ggtgttggct agggaaggtg
     4501 cgaataagca ggttatttct tcccaagctg actcgctgat taaaatttcg cggatctggg
     4561 ccgatttttt tcccgcaaac acatcgaatc agcctattta ggggtcagtt tggagatcgg
     4621 aaatttttgt acgttaagca cattatttaa ccaattcgga tacgtaagtg attgatttat
     4681 cgtcattggc tatcgctttt aggttgtcgc tcttgctgta tcgcatttag gttatccgat
     4741 taagacaaaa tcccctgaaa acgctgtata gcgcgggagt acaccctatt gctacaggta
     4801 agcctgttca aaaaacaggc ttaccgtaca ataattctct atatccaaac tgaccccaag
     4861 aaggacacct tcagcgcggc cgtggtcggt tcgccggtaa agtaggcttt accctcgctc
     4921 tcattccact tgttcttcac agggtcgtag ccgcggttaa gcacgctgat gccgccgtcg
     4981 ctgcgcttgc cgtaggtggc ggtaacctgc tctaggccgc gttcaaagcg gttttccagg
     5041 cgagcgactt cgtaccattt gccaagatag cggctggcgt caaaaccgga aatgggctgc
     5101 acgcccttcg gtggggtagg ggatttacag gctaccagcg tcagggcaat ggcaacaccg
     5161 gtcacaacag gccatagctt catgagaatt cctttcattt gcacggttgg aaattaagtg
     5221 tagtgcaccg cggcggcgct tcatcccatc cgcgaaaatt cgtagtatat tgagagtatt
     5281 cctcaactct ggacacggac atggcttact ccatcggcga attcgccaga ctcagcggga
     5341 tcaccgccac cacgctgcgg gcctggcagc ggcgctacgg cttactcaag cctcagcgca
     5401 cggagggcgg ccaccgccag tacagcgatg aggacgtcca gcaggcgcta aaaatcctcg
     5461 actgggtaaa aaaaggcgtc ccgattggcc aggtcaaacc gctgctggag cgccctacgt
     5521 cgcgcctgac caacaactgg ctcacgctgc agcagagcat gttacagcgc ctgcaggacg
     5581 gcaaaatcga gtccctgcgt cagatgattt acgacgcagg acgcgagtac ccaagaccag
     5641 aactggtcgc gaacgtgctg cgtccgctgc gcagtcaggt gtcggcaaac gtcgccgccg
     5701 ccatgaccct gcgggagatc ctcgacggca tcatcatcgc ctacacctcg ttttgcttgg
     5761 aaggcgataa aaaagcgccg ggcgacaaca tcctgctcag cggctggaac ctgaatgacc
     5821 cgtgcgaagt ctggctcgaa gccttaaccc gcaccgggca aggccaccgt atcgatattt
     5881 tacccacgcc gcccgccacg ctggccccgg agattttccc ggaccgccac tggcttctgg
     5941 tcacgcgcgg taaactcacc gccgcgcgca aaaagcagat cgagcagtgg cagcagcagg
     6001 tatcgcttga agtgattatc ctctaatcat tttctcctgc gggaatcttc acgaaaagtt
     6061 gaatattttc ccgcgccaca aatgctaacg ttttcctgtg accgacaacc ccacaggaaa
     6121 acacaatgaa aaaaactgcc tctctgctga tgctgaccac cctggccttt gcccctgccg
     6181 cctttagcgc ccctgccggc acgctgagcg tacacattct tgaccagcaa accgggatgc
     6241 cgccatcgga cgttaccgtc acgctggaaa aacagcagca ggacaaatgg accccaattg
     6301 ccagcggcaa aactgaccat gacggccgca tcaaatcgct ttatccggag gatcaggata
     6361 tgcagcccgg cgtctataaa gtggccttta aaaccgcaga ttacttccac gggaagaagc
     6421 tggactcctt cttcccggag atcccggtgc tgttcaccgt aacccggacc aacgaaaagc
     6481 tgcacatccc tctcctgctg agccaatacg gttattcaac ttacaagggc agttaagaaa
     6541 cgttgatttt aaaggcaaat aaaaaataat tccgctaata cttggacaaa ttagttatgt
     6601 tgtgtaagtt ttaaagagaa gaacgggagg accacaccat gacaacaaaa cctgtgctcg
     6661 gtattagcgg atgtttgacc gggtctgccg ttcgcttcga cggcggacac aagcgtatgg
     6721 gctttgtcat ggatgaactg gcccagtggg tgagtttcag gcccgtctgc ccggaaatgg
     6781 cgattggcct gccgacgcca cgcccggcca tacgcctgac gctgacggac agcggcgaaa
     6841 cgcagctccg cttcagcaag ccgccccatg atgatataac gcagaaaatg gccgacttta
     6901 cggcggacta tctgccaaaa atcggcgatc tgtcgggatt tatcgtctgc gcgaaatcgc
     6961 caagctgcgg catggaacgc gtgcggctat atgatgaaaa cggcaaccgg ggccgcaagg
     7021 agggtgtcgg gctgttcacc gccgccctgc ttgaaaccta cccgtggctg cccgtggagg
     7081 aagacggtcg gctgcacgac ccggtgctgc gggaaaactt tatcgagcgg gtatttgccc
     7141 tgcacgagct gaacacgctg cgcgctaaag gcctgacgcg ccgcgcgctg ctggatttcc
     7201 acagccgcta taaattgcag cttctggcgc accatcaggc gggctaccgc gagattggcc
     7261 cgttcgttgc gtcgctgcat gaatgggaag atctggacgc gttcttcgtg gcgtaccgcg
     7321 aaaagctgat gaccatcctg aaaaagcccg cctcgcggaa aaaccatacc aacgtgctaa
     7381 tgcatatcca gggctatttc cgtaaccagc tgaacagccg ccagcgcgtc gagctgcgcg
     7441 atgtgatcct gcactatcgc gacgggctgc tacccatcct cgcgccgttg acgctactga
     7501 aacactacat ggccgaatac cctgaccggt atctgatgac gcaaaactac tttgatccct
     7561 atcctgatga tttgggtctg cgtctggcag taacctgacg ataaaaatgc gaaggcccgt
     7621 ttgacccaca gaggcccaaa gcagtacccc gtttagacga caccctcact gtcgtctctt
     7681 ttttgcattc cacacaaaag actgtaatta tgtacaggat tgtaacgcaa aaagggactg
     7741 cctgtgatca ccaccctgca cattcaaaac taccgctcga ttcgcgaaat gtcgctggag
     7801 ctggaacagc tcaacatcgt ctttggccca aacggcaccg gaaaatccaa tatctacaag
     7861 gcgatccacc tgatgcacag cgccgcacag gggcagtttt cgcaggcact ggcgaacgag
     7921 ggtgggattt taaaagtgtt ctgggcaggc aaaacccgca gcgaccagct gcggcggatg
     7981 aatttggccg ttgaaaccga aacctacgaa tacgagctgc aggtaggctt tgtcgaaaaa
     8041 ctgccttacc cctcacagtt tcaactcgat ccggtgatca aagaagagtc catctggctg
     8101 agtggtcaat accgccgccc gtcgtcacag ctgatgaagc gtaaaaatca ggccgtattt
     8161 ctgaacaacg tgcatcacga gaaagtcacc cacagcggca cgctgtatga gaacgaatcg
     8221 gtgttcggcc agcttggcga gccgcatctt tacccggaag tgtcgcagat gcgcgagtcg
     8281 ctgcgtaact ggcgctttta tcaggagttt tctgtatcaa tcggctcggc catgcgcgcg
     8341 cctcaggtcg gtttccgctc cccagtgctg gccagcgacg gcgcaaacct ggcggcggcg
     8401 tttcagacca tcgtggaaat tggcgacgag ctgctgctga tgcgcatcct cgaccaggcc
     8461 ttccccggct gcgtgtttta cagcgacaac accggcggac gcttccggat gatgatgcag
     8521 cgtgaagggc taagcagacc gctggagccg gcggaattct ccgacggcac tctgcgtttt
     8581 ctgtgcctgg cggtggcgct attaagcccg cgtccaccgg cctttatcgc cctcaacgaa
     8641 ccggaaaaca gcctgcatcc gcagatgctg cccgcgctgg cgagcttaat cgccgaggcc
     8701 agccgctact cgcaaatttg gctgaccagc cactcgccgg aactggcgaa tttaattgag
     8761 aaacagagat cgttttcgct gtatcagctg tcgatggtgg aaggagaaac gaaggtggag
     8821 aggctggggt agtctcccag
//
LOCUS       DADLSA010000154         8771 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.155, whole genome shotgun sequence.
ACCESSION   DADLSA010000154 DADLSA010000000
VERSION     DADLSA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.155"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(524..892)
                     /locus_tag="JN691_003460"
                     /pseudo
     CDS             complement(524..892)
                     /locus_tag="JN691_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706202.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1418..2392
                     /gene="hemB"
                     /locus_tag="JN691_003461"
     CDS             1418..2392
                     /gene="hemB"
                     /locus_tag="JN691_003461"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAY7244807.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(2498..3349)
                     /gene="tauD"
                     /locus_tag="JN691_003462"
     CDS             complement(2498..3349)
                     /gene="tauD"
                     /locus_tag="JN691_003462"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HAY7244808.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3346..4173)
                     /gene="tauC"
                     /locus_tag="JN691_003463"
     CDS             complement(3346..4173)
                     /gene="tauC"
                     /locus_tag="JN691_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAY7244809.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(4170..4937)
                     /gene="tauB"
                     /locus_tag="JN691_003464"
     CDS             complement(4170..4937)
                     /gene="tauB"
                     /locus_tag="JN691_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAY7244810.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLLLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4950..5876)
                     /gene="tauA"
                     /locus_tag="JN691_003465"
     CDS             complement(4950..5876)
                     /gene="tauA"
                     /locus_tag="JN691_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7244811.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQVDNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSGID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            complement(6144..6659)
                     /locus_tag="JN691_003466"
     CDS             complement(6144..6659)
                     /locus_tag="JN691_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244812.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAAARAWGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQKTQSDLSQGDQLTEMLSFLTSEER
                     EALTNMLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVREILEEIEAS
                     ILSESVNAKAG"
     gene            6853..7104
                     /locus_tag="JN691_003467"
     CDS             6853..7104
                     /locus_tag="JN691_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7244813.1"
                     /translation="MRQREDSGRDWPSLMIQQALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            7106..8771
                     /locus_tag="JN691_003468"
                     /pseudo
     CDS             7106..8771
                     /locus_tag="JN691_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2206 a         2279 c         2173 g         2113 t
ORIGIN      
        1 tctgcatagc attatcagca ttcggatgtg caactacagc taatgcaaca tcatcccagc
       61 ttgcaatagt gctatcagca acaccatctc cgtcagtatc tacggtgaca aagttagatt
      121 taccactata atcgctggca ttacctgtat taccgaacca accagtagta ccttcatcag
      181 tccatgaacc agaggttacg gtagaatctt taacagtgat ggtgttatta aacacttcgc
      241 tgctgtttac gccagtgctg acagtgttgt cgtcaatatc ttcccactca taaccctgag
      301 tcaacgtaat acccgcaacg tgagagttat tagtgatgga cagatcaact tcctgatcca
      361 gagtaatagc cgtaccaatg ttgtaaacgt ctacaacatg agtatcagcg gcattattgt
      421 aagtgccgtt atatgtgtag tgttcgtagt tgtcgtcgat cgttgaatta tcaacagtca
      481 gcgccagacg gtcatggtag taatcatccg cacgatcagc acaatcagtt gtcatgcact
      541 cggaataaat cataccgtga atagtgctgt ttttgattgt cagactattg gcgttggtat
      601 tggtagaaat accgtcgtcc agatagtagg tagaaatcac gccgttaacg gtagcgttat
      661 taatgaccgg gtagatatcg ccgttgtaat agctatcagc ggcatagttg ttccagccta
      721 cataaccatc atagtaaacg tcatcagcat aggtggcgtc attgtagtga tggaaagtat
      781 tgtaagtttg accagaaata tcgttggtcg ctgcattagc ctgagaggtg atagccagag
      841 tgcaagccaa tgctaattgt gatactacaa gtttcttttt ccaggagtgc atttgtcatc
      901 cctcctcagg gacgtaataa gttgatccat caattatcaa tgcatagaaa acatattatg
      961 tttcgcgcta acgattatgc tgcgttacag acgaatggtt caattattct ttcccctaag
     1021 tccgaatgtg gacatatata atttatggag aaaaacacgc atcaaattga taaaaggacg
     1081 atatttcagc atgttgcaga taatgttatt tttgatacat acgcgttata aatatcaaaa
     1141 acacaaacca gataaatatc ttatcaatat acaacaaagt ttgaatcatt caatttatag
     1201 cagcacaacc aaacgccaaa aaaaataata accaagagct ataaataaag attaaaaata
     1261 ttttcagtga atatgaaatt attgctattt ctattacaaa gatgtcctgg tctctctttc
     1321 attctcttct tgtcaacatc gcgacaactt tcgtaaaaca ctcctgcccc gcttcaggta
     1381 tactgtgcac cttaattcca caaacatcag gcagaccatg acagacttaa tccaacgccc
     1441 tcgtcgcctg cgcaaatctc ctgcgctgcg cgctatgttt gaagagacaa cacttagcct
     1501 taacgacctg gtgttgccga tctttgttga agaagaaatt gacgactaca aagccgttga
     1561 agccatgcca ggcgtgatgc gcattccaga gaaacatctg gcacgcgaaa ttgaacgcat
     1621 cgccaacgcc ggtatccgtt ccgtaatgac ttttggcatc tctcaccata ccgatgaaac
     1681 cggcagcgac gcctggcggg aagatggact ggtggcgcgt atgtcgcgca tctgcaagca
     1741 gactgtgcca gaaatgatcg tcatgtcaga cacttgtttc tgtgaataca cttctcacgg
     1801 tcactgcggt gtgctgtgcg agcatggcgt cgacaacgac gcgactctgg aaaatttagg
     1861 caagcaagcc gtggttgcag ctgctgcagg cgcagacttc atcgcccctt ccgccgcgat
     1921 ggacggccag gtacaggcga ttcgtcaggc gctggacgct gcaggcttta aagatacggc
     1981 gattatgtcg tattcgacca agttcgcctc ttccttttat ggcccgttcc gtgaagctgc
     2041 cggaagcgca ttaaaaggcg accgcaaaag ctatcagatg aacccaatga accgtcgcga
     2101 ggcgattcgt gagtcactgc tggatgaagc ccagggcgca gactgcctga tggttaaacc
     2161 tgctggagcg tacctcgaca tcgtgcgtga gctgcgtgaa cgtactgaat tgccgattgg
     2221 cgcgtatcag gtgagcggtg agtacgcgat gattaagttc gccgcgctgg cgggtgctat
     2281 agatgaagag aaagtcgtgc tcgaaagctt aggttcaatt aagcgtgcgg gtgcggatct
     2341 gattttcagc tactttgcgc tggatttggc tgagaagaag attctgcgtt aatttttttc
     2401 ctctcagaaa gggggagtta acctgttgaa agtacggtga acgccccctc accctaaccc
     2461 tctccccaag ggggcgaggg aaccgtccgc actcgtatca ccccgcccga taaaacggtt
     2521 tatccccaag gatcgtcgcc cgatgcataa tccgccgctg tggcaggtaa tcggcattgg
     2581 catagtgctg agtcacgcgg ttatcccaaa tcgcaatatc attcggttgc cagcgccaac
     2641 gcacctgaaa ctccggttta gtgatatggg cgaataaaaa gcctaacaag gcttcgctct
     2701 ctttctcgct gacatcgaca attcgcgtag taaagccttc attcacaaac agcgcctgtt
     2761 taccgctcac cggatgcgtt cgcaccaccg gatgtagcaa cggaggattt ttcgcgaccg
     2821 cctcgcgcca gcgttgatgt tcctcctcgg ttttgcggta tttgtattcc gggaatgatt
     2881 tacggaaatc atgctccgca cgcagccagc tcagcagctg gcggaaagga acagagagcg
     2941 cctcataggc cgcaataccg ctggtccaga gcgtatcacc gccggtcgaa ggtaactctt
     3001 tagctgccag aatcgcccct gcgggtggcg tttcaataaa tgtcacatcg gtatgccagt
     3061 tgtcgttatc cggcggatta tcgttatggg tatccagcac gatgatctcg tcaacccctt
     3121 cggcatgagg gtaaacaggg tgaatatgca gttcgccaaa acgctgggcc agcgcgcgtt
     3181 gctgctgcgg cgtaatagct tgatcgcgca gaaataccac ctgatggcgc agcaccgcat
     3241 ggtaaagctg ttcaaactga ttatcgctta acgggcgcgt caggtcggca cccgaaattt
     3301 gtgcgccgat atacggcccc agcggggtaa tgctcagacg ttcactcatt gtacttctcc
     3361 atgccagggc gtcagacggc gctgtaacgc gcgcagaccc agttctaaaa gaaaggcgat
     3421 aatcgcaatc accgcgatcc ccgccagtac cacgtcagtc gcgagaaatt cacccgctga
     3481 ctgaaccata aatcctaaac cgcgtgtcgc ggcaatcagc tccgccgcta ccagcgtaga
     3541 ccagcccaca cccagaccaa tgcgtaatcc ggtgaggatt tccggcagcg cgccgggcaa
     3601 aatgacaaac cacagcacct gcgcacggct ggcacccagc gactgggcgg cacgaatgcg
     3661 aacctgctgc gcgcttttca cccctgccag cgccgacatc gccaccggcg caaaaatcgc
     3721 taaatagatc agtaagatct tcgaggtttc accaatacca aaccagatca ccatcagcgg
     3781 caaataagcc agcggcggca ccggacgata aagttcgatt accggatcca gaatgccgcg
     3841 caccgtcggg cttagcccca tggcgatccc gaccggaata ccgatcacca ccgccgcgag
     3901 tagcgccagc acaatgcgcg tcaggctggc tgccagatgc tgccacagcg tggcgtccat
     3961 aaatccctgc ggtccggcaa tggtgagcag ttttgccagt acttgttgcg gcggcggcaa
     4021 aaacagcggg ctaatcagtt gcagcgccgc caccgtccac catacagtga gtaaaaccgc
     4081 taacgtgcca atgcttaagg tcacctgacg cgagagcggc cagcgccatt tcagccgccg
     4141 cgaatgcagt ttttcattaa tgagcacact catgagaacg cctcccgttg ctcaaatacg
     4201 cggcttaaaa catattcgcg catggcgatg aattgtggat cggacttgat gctgcggctc
     4261 gactctcccg caacaaagcg gcgagcaaag ttgagcagca gccgctccag cacacggcca
     4321 gggccggatg aaagcagaac cagttcagtc gccataaaca ccgcttcttc tatatcgtgg
     4381 gtaatcaaca gcacctgctt gcccgtctcc tgccagagtt tcagcagcag agtttgcatc
     4441 tggtcgcggg tgaaggcgtc cagcgcacca aacggttcgt cgagtaataa cagctgggga
     4501 ttcgccgcca gcgcgcgggc aatccccacc cgctgacgtt gaccatcgga aagctgccag
     4561 atatagcgtt tttctgcgcc ttccagcccc acttttttca gcatctggtg cgcgatttcc
     4621 agtcgctgca ttttctctat acctgccaat tgcaggccga acgccacgtt gtcctgtaca
     4681 ttgcgccacg gtagcagccc ttcattctga aaaactacgc cacgctctgc tcccggtccc
     4741 tcaatacgct tacccgccag ttgaatgctg ccatgctgat aaggcacaaa accggcaatc
     4801 agattcagca gggtggtttt accgcagccg gacggcccca gcaccaccag tagctcgccg
     4861 ctttccagcg tcaggttgat atcttccagt gccggtttgc cgccataatc ggcatagaga
     4921 tgagagattt gcagcatctg cgcctccttt tattgcacga agcgcgaggt aacgtactgg
     4981 ctgtaatcat tcgctacagc cgggaccttg ccctgctctt tcaaaaactg cgcggtgtcg
     5041 atgattgctt tgttcaccgg tccggtcagt tctgccgttt gttgctgcgg cgtcagatag
     5101 gtatttccct tcaccagccc cggaacgtca ccttcaggca cgccgcttaa acgcgccagt
     5161 ttgctgatgt tttccggctg tttcagccac gcgtcagggt tagcaatgta cggttgctgt
     5221 gcatcgatgc cgcttttggc gaacgctttc acgacctcag gatgtttctc ggcaaaatct
     5281 ttgcgcacca cccagacgtc cagcgttggc gcaccccact gcccgacctg ttcagaatcg
     5341 gtcagcacct tgccgtcttt ttccagggcg ttaaccgccg gtgcccagac ataagcacca
     5401 tcaatatcac cccgctgcca tgcggcgata atcgcgggcg gctgcaggtt cacaatctcc
     5461 actagccctg gtttaatccc ccagtgtttc agcgccgcca gcaggctgta gtgggtggtg
     5521 gagataaacg gtacggcaat gcgtttacca atcaaatctt cgggtttgct gatagttttc
     5581 tttaccacca gcgcttcgga gttacccagt tttgacgcca gcaagaagac ttcaatcggc
     5641 acctgttggc tggctgcaac cgctaatggg ctggaaccga ggttgccgat ttgcacgtcg
     5701 ccagaagcca gcgcccgcac gatgctggct ccgctgtcaa acttacgcca gtccacgctc
     5761 gccccgctct ctttagcaaa ggtgttgtcg acctgagcca ctttcgccgg ttcggctgag
     5821 gtttgatacg ccacggtgac gttcaccgcc tgtgcctgaa aagcgatgaa tgccagtgcg
     5881 gcaagaagtg tgttatatga ctaggtaatt tattcaagat aaccgggaat gggtaaaaaa
     5941 agacgggatt tgatgggatt caataagtta tgcgccgatt gcattcaaga taaaacgagt
     6001 taaaaatgct caatgcttcg atatcgataa aacattcaag ataggcgcgc cgtgtatttt
     6061 aattctttta agactcttca aaaatgtcat cgtagccttt aaaaacgaat tttaaaggcg
     6121 accctaaagg caggtcaatg aagttaaccc gccttcgcgt ttacactctc tgagagaata
     6181 ctagcttcaa tctcttccaa aatctctctg acttgctcgt cgggcatatc aacgagcatc
     6241 aacgctgcca ggcgtttacg gccctctagt tgcagtagct cttgagtttc cttatctaac
     6301 aagcgtagca cagtctcaac acctttcctt gcgagcatgt ttgtaagggc ttctcgttcc
     6361 tcactggtaa gaaacgacaa catttctgtt agctgatccc cttggcttaa atcactttgt
     6421 gttttttgtg tttttggtgg attcccgtta ccggttttta gccattcaag actaacacct
     6481 tcactttctg caatgcgtac caatatgctt tctcgaggtt ctggtgtatt tcccttttta
     6541 aaatagcttt gtaacgtatt gatatttatg ccccaggctc tggcggcagc acttcttgat
     6601 ggataccgcc taataagata agccatgcgc tccacgatag gttcttttgc ctgctccata
     6661 aaagtacctc taggtatttt tctgtataaa ggttctttcc catctatcta attgaatgga
     6721 aaggtaattt taaaaatcca aataaaatac aaaaaaaata ccttgtattg gtgctttttg
     6781 tgatctgcga ttttatttac tcggatatcc acgcgaatat ccgcgcgggt aattttgtag
     6841 ggtaaaggaa agatgagaca aagagaagat agtggccgcg attggccttc gctgatgatt
     6901 cagcaagcac tcaaagataa tgggtggtct tcctttcggg agctttcact ccatgccggt
     6961 tatgctcagg attcactgaa agcggtagtt cgtacgccaa acaaaaaata cgaacgtgtt
     7021 atttctgacg ctataggtgt accgccagaa atcatctggc ccagccggta tcgtaacgat
     7081 ggatttatca ggaaggcgtc gtgatatgca gtcgtacgtt accgtaaatg acctgctcgg
     7141 tgttcctgga atgccagcca ccgcgaaagg catccgacag gcgttacagc gtttttctcg
     7201 tgctcttggt gacgttagtc gccgtcgagc aggtacaaaa gccattgagt accatatcga
     7261 ttgcctgcct aaggtaaccc aaaaggcact gcgtgaacgc tatgtggaac aactggtggc
     7321 tacagagaac aacgtctccg aagtgaagac cgttaccagg acggcgcgta accctgatgc
     7381 tgttcaggca gttgaagcat atcggggttc tccgcaacta atggaagaac gcctgaatgc
     7441 cctgactgaa aatcagcgcc gggtgtctga agcaagagcg gcgctggcag tggaagtgct
     7501 gaagctggaa agcgccggta acctcgggcg actgaaagcc attaactttc tggttgaaaa
     7561 agcccgtaaa ggtgagttgc cggaacgact gcaacaggct gcgattaacg ctaatgcaaa
     7621 acgtggcact aatcgcacca tcagccgtga tccgctttac cagtgggtac tgaaatacaa
     7681 ccggtcgcag aacgccgcag aacgactgtt gttactggca ccaggaaaac gcgacgaaat
     7741 aaaaccggaa gaaattagct ggctgcctga atttctggcg cagtatcgtc aggctaacgg
     7801 cagacctatg tcggaagcct acgaggattt tgtcacggaa tggcaacgtc gctatgcgga
     7861 cgaaccgtac atgctggagg tgatgccttc ctatgatgtt gtccggtatg ccatgaaaaa
     7921 attgccggaa gtggtgaaac aaaaaggacg cgttaccggc agtgaatacc gccagcttga
     7981 gggattcacc cgccgcgact ggacagctat gccggtgaat tatgtctgga ttggtgacgg
     8041 ccacggtatg aagctgaaat gtgcgcatcc gatccacggg cgtccatttt caccggaagt
     8101 cacgtttgtt atcgacggtg gaacccggtt tgttgttggc tggagtcttg atcttgcgga
     8161 gaacgtgttt gctgttgccg gggcgataca gcacggaatt cgcaatcacg gaaaaccctt
     8221 cctgtattac tcggataatg gttccggtga aacggcagat atgctggata agagatcgtc
     8281 gggattctgc cccgtctggg tattaaccac cccaccggta ttgccggaaa tccgcaggga
     8341 cgcggcatca ttgaacgtct gaaccggacg ctgccgatgc gtatagccag acggtatcgc
     8401 acctattttg gcaagggggc agaccgggaa acactgcgtg tactcaatcg cgatctgcgt
     8461 tcagcattta atgccctgca acaggacaaa ccgctgaatg cccgccagaa atctgcaatg
     8521 cgcgagctgc cgtcctgatc agaactgatt gaggctattc gtgaaggagt ggaatggtac
     8581 aacaaccgcc cacattctga actgccgatg aaaccaaacg ggaagcatta cagcccggct
     8641 gatttcagaa aaaaacgcct ggcggaagaa aacaccgaaa ttgaatggct gagtgatctc
     8701 gaactgcgcg acatgttctg cccgatggtg gagcgtccgg taagacgctg tgaaatcaaa
     8761 tggcttggga a
//
LOCUS       DADLSA010000155         8555 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.156, whole genome shotgun sequence.
ACCESSION   DADLSA010000155 DADLSA010000000
VERSION     DADLSA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.156"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..649)
                     /gene="yihS"
                     /locus_tag="JN691_003469"
     CDS             complement(<1..649)
                     /gene="yihS"
                     /locus_tag="JN691_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HAY7244814.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRV"
     gene            complement(666..1544)
                     /gene="yihT"
                     /locus_tag="JN691_003470"
     CDS             complement(666..1544)
                     /gene="yihT"
                     /locus_tag="JN691_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HAY7244815.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(1568..2464)
                     /gene="yihU"
                     /locus_tag="JN691_003471"
     CDS             complement(1568..2464)
                     /gene="yihU"
                     /locus_tag="JN691_003471"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAY7244816.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVARTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            2632..3528
                     /gene="yihV"
                     /locus_tag="JN691_003472"
     CDS             2632..3528
                     /gene="yihV"
                     /locus_tag="JN691_003472"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HAY7244817.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITSQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            3562..4347
                     /locus_tag="JN691_003473"
     CDS             3562..4347
                     /locus_tag="JN691_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7244818.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            4446..5045
                     /gene="yihX"
                     /locus_tag="JN691_003474"
     CDS             4446..5045
                     /gene="yihX"
                     /locus_tag="JN691_003474"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAY7244819.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            5039..5911
                     /locus_tag="JN691_003475"
     CDS             5039..5911
                     /locus_tag="JN691_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HAY7244820.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            5908..6345
                     /gene="dtd"
                     /locus_tag="JN691_003476"
     CDS             5908..6345
                     /gene="dtd"
                     /locus_tag="JN691_003476"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAY7244821.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            6390..7331
                     /gene="fabY"
                     /locus_tag="JN691_003477"
                     /gene_synonym="yiiD"
     CDS             6390..7331
                     /gene="fabY"
                     /locus_tag="JN691_003477"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAY7244822.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            7747..8555
                     /locus_tag="JN691_003478"
                     /pseudo
     CDS             7747..8555
                     /locus_tag="JN691_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2090 a         2071 c         2240 g         2154 t
ORIGIN      
        1 taacgcgata atgattattt ctggcgacgt cgtggataat cacggaagcc acgcgaatcg
       61 cgcgatccag ccattttttg tcgtgagtga cgtcataaac aatcaagaaa gcttccaccg
      121 cgtgcatatt ggcattgccg ccgcggtact cttcggtttt gctgaaggct tcgtcccagg
      181 attccaggca catctgctct tcttcgctcc agaaatattt ctcgataatc tcaatggtgt
      241 aatcgagcag cttgcgcgct tccgggtgac ccgttgtgac ggcgctggcg gcacccagca
      301 gagcaaagaa gtgttgatag ccctgtttgg aggcatccac cacgccctcg tcattcacgc
      361 aggcatacca gccgccatat tttttatcac gcagtgcgcc gttcatggct ttaataccgt
      421 gatcaaccaa cgcgtaagcg ccaggtcgac ccatcgccgc agcaacggaa taaacgtgca
      481 acatacgagc tgtgatccac agatgggtgc ccatctcttc tttgatttgc cctttattgc
      541 ctaaccagcc aaaaccagtc ggcactacgg aatttttacc aaaatcaaag atgcggtcgg
      601 tttcctgttc cagccaacgg ttgtggctta gggtgttaaa ccatttcatt ccttttatcc
      661 tcatcttagc ggcgtttagc catcatttcg tcgacgatat cgccaagttg ttgtaatttc
      721 ggtgcgcaaa cgtcacggag catcagctcg ttgtctggta aaccgacgac cgatgcccag
      781 actgcacggc ctgccaggaa tcccgatgcg cccgccgtca ttgccacgcg tacggcacgc
      841 gggaacagtt tttcgtcgac accggaagag aggatcaccc atggcatatt gatatggtca
      901 ttcagacgtt gtgaagcaca gagaagctct tgttgcggac ctttgccata aagcggcatt
      961 tcaactttgt agaggtcagc gccactgtcg cccagctctt tggcggcatc gatgatcgct
     1021 tgttcgcgat cgaatttatc gccacgacgc ggtgggcgga cgactggctc aatgatgctt
     1081 accagaccgt gtgagtggca cagttcgttg aactctttca ccatatccag acgttgctgc
     1141 gcatcttcat cactacgcca cagcaccagc agttttaagg ctttgccacc gtcttgtttg
     1201 atctgtagcg gattgatttt gcggtcaata accacgctat cgaccggaat accgttgcca
     1261 ggaatgaact catcggcggc gacaatcatg gcgcaacttt tggcaatcgc gttttgctca
     1321 accacctggc ggtagcagaa ttgttgatct accagaatcg ccgaggcata aggcgagagg
     1381 gcctttgcag cgttaacttt gaaatcagtt aaaacgctat cggctaccgg agcgggtgcc
     1441 ccagccgcgg caaacatcat gcgcatggct tcgcgctgat cgaccgccag catggcaaaa
     1501 ccgcccgatg cgcgcgtaat gtcgttgatg gtgtacttat tcattcattt attcctttat
     1561 ccagtcgtta catttttact ttggcagtca tcccggcact gacacggacc tgttccagaa
     1621 tggcggacca gtcctggcga ccgcgacccg ctgcgcgcgc ctggctataa acctcccgtg
     1681 aggcggcccc cagcggcatt ggcacatgca gctggttggc gacatcaagg gcgatgccaa
     1741 gatccttatg ggcaagatcg atcatgaagg cgggagaaag atctccgctg aggactttgt
     1801 ttggccagga agtggtgaag tgacctttac cggcggcggt accgctcatc actttgacgg
     1861 caacatcgaa gggaagattc agggcttcgc acaaaacggc ggcttctgcc gaaagcgcat
     1921 tgagcgcgat gctcatgtag ttgttgatga gcttaacgcg gatccccatg cccggaccgc
     1981 cagcgttgat caactcactg cccatcgcca tcaggatcgg tgtggcacgt tcaacttgtt
     2041 cagcggtgcc gccagccagc agtaacagag taccagtaat ggcatttgca gaagtacggg
     2101 ctaccggaac atccatcatg ctgaagcctt tggcttgcat atcggcaatc aatttatcgg
     2161 tttgcagcgg atggatggtg gacatatcaa tgaccagcgc atcggtagat aagccttcgc
     2221 aaacgccgtt ttcaccgaac aacacgttgc gcaccagatc gccattcggc aacatggtaa
     2281 tgataaattc ggcatcttta gcggcctgcg ccgggttggc ggcgggagtc gcacctttgt
     2341 ctaccagatg ccgcacagct tcggcgttca catcaaagac gcgaagttga tgcccttgct
     2401 gcaataaatt gctcgccatt ggcgaaccca tttgtcctaa accgataaat gcgattgctg
     2461 ccataaccct ctcctgaata cagtgatgtc actttttgtc atttatgaca tgctttgctt
     2521 gtctgttttt gatcgcattt gtaatttatc gtcaaaaaat tgacagccgt cactttttaa
     2581 acaattggtg aaattaaaat gaacgcatcc caaaatgttt aaggaatgac catgattcgt
     2641 gttgcttgtg taggtataac cgtgatggat cgcatctatt acgtggaagg gttaccgacg
     2701 gagagcggta aatacgtggc gagaaattat acggaagttg gtggcgggcc agcggcgacg
     2761 gcagcggttg cggcggcaag gctgggggcg caggtcgatt ttattggtcg cgtaggtgat
     2821 gacgacgcgg gcaacagcct gctggcagag ctggaatcct ggggcgttaa cacccgttac
     2881 accaaacggt ataaccaggc gaaatcttcg caatccgcca tcatggtgga taccaaaggc
     2941 gagcggataa tcattaacta ccccagcccg gatctgctgc ctgacgcaga gtggttggag
     3001 gaaattgatt tctctcagtg ggatgttgtg ctggcagatg tacgctggca cgacggcgct
     3061 aaaaaagcct tcaccctggc ccgtcaggcg ggtgtgatga ccgttctgga cggggacatt
     3121 acgtcgcagg atatcagtga gctggtggca ttaagcgatc acgcggcctt ttcagaaccg
     3181 ggtctggcgc gcttaacggg cgtgaaagag atggccagtg cgctaaaaca ggcacaaacg
     3241 ctcacaaatg gtcatgtcta tgtgacccag ggtagcgcag gctgcgactg gctggaaaat
     3301 ggtgggcgtc agcatcaacc ggccttcaaa gttgatgtgg tagataccac aggtgcgggt
     3361 gatgtttttc acggcgcttt ggcggtggcg ctggcaacaa gtggggattt agcggagtca
     3421 gtccgcttcg ccagcggtgt agcggcgtta aaatgcacac gtcccggtgg acgagccggg
     3481 atccctgact gtgatcaaac ccgatctttt ttgtcacttt ttgtataaaa tgccagggtg
     3541 atggtttttc gaggaatttt catgagcctt accgaactaa ccggtaaccc gcggcacgac
     3601 caactcctca tgctgatcgc cgagcgtggg tatatgaata ttgatgagct ggcaaatctg
     3661 ctggatgtct ccacgcagac ggtccgccgg gatattcgta aattaagcga gcaaggcctg
     3721 attacgcgcc atcacggtgg cgcgggtcgg gcttccagcg tcgttaatac ggcgttcgag
     3781 cagcgtgagg tttcgcaaac cgagaaaaaa aaagcgattg ccgaagcggt ggcagactat
     3841 attcctgatg gttcaacaat atttatcacc attggtacga ctgttgagca tgttgcccgg
     3901 gcgttactta accataatca tttgcggata atcaccaaca gcctgcgtgt ggcgcatatt
     3961 ctttaccaca acccgcgctt tgaagtgatg gtgcccggcg gtacgttgcg ctctcataat
     4021 agcgggatca ttggcccttc agcggcgtcc tttgtggctg attttcgtgc tgattatctg
     4081 gtaacaagcg ttggggcgat tgagagcgat ggcgcgttga tggagtttga tgtaaacgaa
     4141 gctaacgtgg tgaaaacgat gatggcgcac gcgagaaata ttctgctggt cgccgatcac
     4201 actaagtatc atgcttcggc ggcggttgaa attggtaacg tggcacaggt cactgcgctc
     4261 tttaccgacg agctgccgcc cgctgcgcta aaatcacgct tacaagacag ccaaattgaa
     4321 atcattcttc cccaggaaga cgcgtagatt tttgtgaccg ttaacctggc ttcatacctt
     4381 gccacatagc caaacccatc ctttcccgct acagttaatt tcttgtggcg cgaaaggagg
     4441 caaaaatgct ctatatcttt gatttaggta atgtgattgt cgatatcgac tttaaccgtg
     4501 tgctgggagc ctggagcgat ttaacgcgta ttccgctggc atcgcttaag aagagttttc
     4561 atatggggga ggcgtttcat cagcatgagc gtggggaaat tagcgacgaa gcgttcgcag
     4621 aggcgctgtg tcatgagatg gctctaccgc taagctacga gcagttctct cacggctggc
     4681 aggcggtgtt tgttgcgctg cgcccggaag tgatcgccat catgcataaa ctgcgtgagc
     4741 aggggcatcg cgtggtggtg ctttccaata ccaaccgcct gcataccacc ttctggccgg
     4801 aagaataccc ggaaattcgt gatgctgctg accatatcta tctgtcgcaa gatctgggga
     4861 tgcgcaaacc tgaagcacga atttaccagc atgttttgca ggcggaaggt ttttcaccca
     4921 gcgatacggt ctttttcgac gataacgccg ataatataga aggagctaat cagctaggca
     4981 ttaccagtat tctggtgaaa gataaaacca ccatcccgga ctatttcgcg aaggtgttat
     5041 gctaaaaacc attcaggaca aagccaggca ccgtacccgt ccactatggg cctggctaaa
     5101 actactctgg caacgtattg atgaggacaa catgacaacc ctggcaggta accttgccta
     5161 tgtgtcgttg ctctcattag tgccgctggt tgccgttgtt tttgcgcttt tcgccgcttt
     5221 tcccatgttt tccgacgtca gcattcagtt acgtcacttt atttttgcca actttctgcc
     5281 tgctactggc gatgttattc agcggtatat cgaacaattt gttgctaatt ccaacaagat
     5341 gaccgccgtt ggggcgtgcg ggctgatcgt caccgcgtta ttgttgatgt actccatcga
     5401 tagcgcgttg aataccatct ggcgcagtaa acgagcgcga ccaaaaattt actcgttcgc
     5461 cgtgtactgg atgattttaa cgctggggcc gctgctggca ggggccagtc tggcgatcag
     5521 ttcctatttg ctctccctgc gctgggcgag cgatctcaat actgtcatcg acaacgtgct
     5581 gcgtattttt ccgttgctgt tgtcgtggat ctccttctgg ttgctgtaca gcattgttcc
     5641 taccatccgc gtacctaacc gcgacgcgat tgtcggcgcg tttgtcgccg cactcctgtt
     5701 cgaagcagga aagaaaggtt tcgcgcttta tatcaccatg ttcccgtcat atcagctcat
     5761 ttacggtgtg ctggcggtga tccccattct ctttgtttgg gtctactgga catggtgtat
     5821 cgtcttgctt ggcgcggaaa ttactgtcac tctcggggaa taccgcaaac taaaacaagc
     5881 agctgaacaa gaagaagacg acgaaccatg attgcattaa ttcaacgcgt aacccgtgcc
     5941 agcgtcaccg tggagggaga agtgacgggc gaaattggcg cgggactttt ggtgttattg
     6001 ggtgtcgaaa aggatgacga cgaacagaaa gcaaaccgtc tgtgcgagcg tgtgctcggc
     6061 taccgcatct ttagcgatgc cgaaggcaag atgaatctca acgtgcaaca ggcgggcggc
     6121 agtgtactgg tggtttccca gtttaccctc gccgcagata ccgaacgggg gatgcgccca
     6181 agtttctcca aaggcgcatc accggatcgc gcagaggcgt tatatgacta tttcgtcgaa
     6241 cgctgccgcc agcaagagat gaacacgcaa acaggacgct tcgctgccga tatgcaggta
     6301 tcgctggtca atgatggccc cgtgacattc tggttgcagg tatgagccag cttccagggt
     6361 tttcacggga aacaagagag agtatcgcta tgtatcacct tcaggttcca caaacagaag
     6421 aagagttaga gcgttactat cagtttcgct gggaaatgtt gcgtaagccc ctgcatcaac
     6481 caaaaggttc ggaacgcgac gcgtgggatg cgatggcgca tcaccagatg gtcgtcgacg
     6541 agcagggtaa tctggtggcg gtaggccgat tgtatattaa tgccgacaat gaagcgtcca
     6601 ttcgctttat ggccgttcac ccggacgtgc aggacaaagg gttaggaacg ctgatggcga
     6661 tgaccctgga gtcggtggcg cgtcaggaag gcgttaagcg cgtgacctgt agcgcccgtg
     6721 aagacgcggt ggagtttttc gccaagctgg ggtttgttaa tcagggagaa atcaccacgc
     6781 caaccaccac gccgattcgc cattttttga tgattaagcc cgtcgccact ctggatgata
     6841 ttttgcatcg cggcgactgg tgcgcgcagc tgcaacaggc gtggtacgaa catatcccgc
     6901 ttagtgaaaa aatgggcgtg cgtattcaac aatataccgg gcaaaaattt atcaccacca
     6961 tgccggaaac cggcaatcag aatccgcacc atacgctgtt tgccgggagt ttattctcgc
     7021 tggcgacgct caccggttgg gggcttatct ggctgatgct gcgtgaacgc cacctcggcg
     7081 gaacgattat tctggcggat gcgcatatcc gctacagcaa accgattagc ggtaaacccc
     7141 atgcggtagc cgaccttggt gccttaagcg gcgatctcga ccgtctggcg cgcggacgaa
     7201 aagcacgggt gcagatgcag gtcgaaatct ttggcgacga gacgccgggt gcagtgtttg
     7261 aaggcacgta tatcgttctg cccgcgaagc catttggccc gtatgaagag ggcgggaacg
     7321 aagaagagta gcttatggag aggggcggtg ctgcctctct cattcattcc ggtcatttac
     7381 atcagtccct gactattccg gttatattga ataagaactt tataaatttt tattcttcgt
     7441 tggcaccata cgtgtcttgc tacatcactg ttcaggagca tttaaatatt atttgtagag
     7501 ctgagtggca attattgcag ataatgctta actcatcatc ttagttcagg tttgagcgat
     7561 ttttgatagt caaaaccccc tctggtgcca tacttttttc ataaatattt ttaaaatcta
     7621 tagctcacaa acattatgca aagataatgg gctggcatac attgtcagta tcattcattt
     7681 actgctctgt atgttatgaa tcattatgtt gtatgtttgc tcttaaaccg tgggaaggag
     7741 gctataatgg ttgccaatat taatctcatt aaacaagaaa gttattctgt tgttaattta
     7801 gaaaaacaac taatgagtaa taaatcaccg aagcaaacgc tattaaatca aatttcactt
     7861 tgccttaaaa tggaaggtga ggagcaactg tgcgattgtg ttaacgaact gaagcatggt
     7921 ttgtcgagta ataatgaata tgacctgttt aaaaatagag aaacctcact gtggaaacgc
     7981 aaagtaccag aaagcgcagc gctggcaatg acgggaacag tatataaatt atttggtaat
     8041 cgcttactca acgcccgcaa tcagttagat aaaaatctcc tcgaaatggc tatgcaaatg
     8101 gtttatggca aagatatggt gacaaagtcc ggggatatat tgattgatat acaattaaat
     8161 aaggatggca gtctccagag taagcactac acacttgatg taggtgaagt tataaacagt
     8221 tataatattg accctctttg tgaatgcttc aagtattaat caaacttact tacatgataa
     8281 aaatcctggt gacgttatta gcctgtatgg cactttagat aacattcccc ttgtagttaa
     8341 ggaatgcacg ggtaaaaatc atcaaattaa tgttaaagcc aaattgccac aagaacgaag
     8401 tgaaaaagtt gaggatatgt gtgaaagaat gaaaggtggc atctctatgt ttaaccacac
     8461 cacaaaaaca gctgataata tagagcataa tttgcagcta agttttctcg tgggttcacg
     8521 tccgtcggtc accaataaat actcagcaaa agatg
//
LOCUS       DADLSA010000156         8546 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.157, whole genome shotgun sequence.
ACCESSION   DADLSA010000156 DADLSA010000000
VERSION     DADLSA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.157"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            128..1534
                     /gene="fliD"
                     /locus_tag="JN691_003479"
     CDS             128..1534
                     /gene="fliD"
                     /locus_tag="JN691_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAY7244823.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     ALAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            1559..1969
                     /gene="fliS"
                     /locus_tag="JN691_003480"
     CDS             1559..1969
                     /gene="fliS"
                     /locus_tag="JN691_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAY7244824.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            1969..2334
                     /gene="fliT"
                     /locus_tag="JN691_003481"
     CDS             1969..2334
                     /gene="fliT"
                     /locus_tag="JN691_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HAY7244825.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            2412..3899
                     /gene="amyA"
                     /locus_tag="JN691_003482"
     CDS             2412..3899
                     /gene="amyA"
                     /locus_tag="JN691_003482"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY7244826.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(3933..4346)
                     /locus_tag="JN691_003483"
     CDS             complement(3933..4346)
                     /locus_tag="JN691_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7244827.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            4533..5738
                     /gene="yedE"
                     /locus_tag="JN691_003484"
     CDS             4533..5738
                     /gene="yedE"
                     /locus_tag="JN691_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAY7244828.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            5735..5968
                     /gene="yedF"
                     /locus_tag="JN691_003485"
     CDS             5735..5968
                     /gene="yedF"
                     /locus_tag="JN691_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAY7244829.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            6075..6745
                     /locus_tag="JN691_003486"
                     /pseudo
     CDS             6075..6745
                     /locus_tag="JN691_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(6785..7993)
                     /gene="tnpB"
                     /locus_tag="JN691_003487"
                     /pseudo
     CDS             complement(6785..7993)
                     /gene="tnpB"
                     /locus_tag="JN691_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
     gene            8001..8430
                     /gene="tnpA"
                     /locus_tag="JN691_003488"
                     /pseudo
     CDS             8001..8430
                     /gene="tnpA"
                     /locus_tag="JN691_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2086 a         2196 c         2180 g         2084 t
ORIGIN      
        1 gacgaaatac tcttttctct gccccttatt cccgctatta aaaaaaacaa ttaaacgtaa
       61 actttgcgca attcaggccg ataaccccgg tattcgtttt acgtgtcgaa agataaaagg
      121 aaatcgcatg gcaagtattt catcgctggg agtcgggtca ggtctggatt taagttcaat
      181 ccttgatagc ctcaccgccg cgcaaaaagc gacgctaacc cctatttcaa atcagcaatc
      241 gtcgtttacc gctaaactta gcgcctacgg tacgctgaaa agcgcgctga cgactttcca
      301 gaccgccaat actgcattgt ctaaagccga tcttttttcc gccaccagca ccaccagcag
      361 caccaccgcg ttcagtgcca ccaccgcggg taacgccatc gccgggaaat acaccatcag
      421 cgtcacccat ctggcgctgg cgcaaaccct gaccacgcgc accaccagag atgatacgaa
      481 aacggcgatc gccaccagcg acagcaaact caccattcaa caaggcggcg acaaagatcc
      541 gattaccatt gatatcagcg cggctaactc atcgttaagc gggatccgtg atgccatcaa
      601 caacgcaaaa gcgggcgtaa gtgcgagcat cattaacgtg ggtaacggtg aatatcgtct
      661 gtcagtcaca tcaaatgaca ccggccttga taatgcgatg acactctcgg tcagcggtga
      721 tgatgcgcta caaagtttta tgggctatga cgccagtgcc agcagcaacg gtatggaggt
      781 ctcggtcgcc gcccagaatg cgcagctgac ggtcaacaac gtcgccatcg agaacagcag
      841 caacactatc agcgacgcgc tggaaaacat caccctgaac ctgaacgatg tcaccacggg
      901 caaccagacg ctaaccatca ctcaggacac ctccaaagcg caaacggcga ttaaagactg
      961 ggtgaatgcc tataactcgc taatagatac cttcagcagc ctgaccaaat acaccgccgt
     1021 agatgcggga gctgatagcc agagttctag caatggcgca ctgctcggcg actccacgct
     1081 gcggacgatt cagacgcagt tgaagtcgat gctgagtaat accgtcagtt cttccagcta
     1141 taaaacgctg gcgcagattg gtatcacgac cgatcccagc gatggcaaac tggaactgga
     1201 tgccgacaaa ctcaccgctg cactgaaaaa agatgccagc ggcgtaggtg cattgattgt
     1261 tggcgatggt aaaaaaaccg gcatcacaac caccatcggc agcaacctga ccagttggct
     1321 ttcgacaacg ggcattatta aagccgctac cgatggcgtt agtaaaaccc tgaataagtt
     1381 aactaaagac tacaacgccg ccagcgatcg cattgatgcg caggtcgcgc gctacaaaga
     1441 acaatttacc caactggacg ttttaatgac ctcgttaaac agcaccagca gctacttaac
     1501 gcagcagttc gaaaacaaca gtaattccaa gtaatcatta ttcatcggga gacaggtcat
     1561 gtacgcggca aaaggcaccc aggcctatgc acaaattggc gtcgaaagcg ccgtaatgag
     1621 cgccagccaa cagcagttgg tcaccatgct atttgatgga gtgctgagcg cactggttag
     1681 agcgcgactg tttatgcagg acaacaatca gcaaggcaaa ggcgtctctt tgtcaaaagc
     1741 gatcaacatc attgagaacg gactgcgggt gagtcttgat gaagagagca aagacgaatt
     1801 aacccaaaac ttaattgctc tttatagcta tatggtcagg cgtttgctgc aagccaattt
     1861 acgcaacgat gtctccgcag tcgaagaggt ggaagcatta atgcgtaata ttgccgatgc
     1921 ctggaaagag tctttactct ccccttcttc gattcaggac ccagtctgat gaacaatgca
     1981 ccgcatttgt atttcgcctg gcaacaactc gtcgaaaaaa gccagctcat gttacgcctg
     2041 gcaacggaag aacaatggga cgaactcatc gctagcgaaa tggcgtatgt gaatgcggtg
     2101 caggagatcg cacacctgac tgaagaggtt gccccgtcca ctacgatgca ggagcagctc
     2161 cgcccgatgc tgcacctgat tctcgacaac gaaagcaagg taaagcagtt attacagatt
     2221 cggatggatg aactggcgaa actggtcggt cagtcatcgg tgcaaaaatc ggtgttaagt
     2281 gcctatggcg atcagggcgg ctttgtgctg gctccgcagg ataacctctt ttgaatctta
     2341 atgagccgat ggctcgcgaa taatccgatt acggctacgc ttctaatatt ccccttgaat
     2401 ggagtcgaag aatgcgtaat cccacgctgt tacaatgttt tcactggtat tacccggaag
     2461 gcggtaagct ctggcctgaa ctggccgagc gcgcagacgg ttttaatgat attggtatca
     2521 atatggtctg gttgccgccc gcctataaag gcgcatcggg cggctattcg gtcggctacg
     2581 actcctatga tttatttgat ctaggtgagt ttgatcagaa aggcagcatc cctactaaat
     2641 atggtgataa agcacaactg ctggccgcca ttgacgccct gaaacgtaat gacattgcgg
     2701 tgctgctgga cgtggtagtc aaccacaaaa tgggcgcgga tgaaaaagaa gctattcgcg
     2761 tgcagcgtgt aaatgctgat gaccgtacgc aaattgacga agaaatcatt gagtgtgaag
     2821 gctggacgcg ttacaccttc cccgctcgtg ccgggcaata ctcgcagttt atctgggatt
     2881 tcaaatgttt tagcggtatc gaccatatcg aacaccctga tgaagatggc atttttaaaa
     2941 ttgttaacga ctataccgga gaaggctgga acgatcaggt tgatgatgaa ctcggtaatt
     3001 tcgattatct gatgggtgag aatatcgatt ttcgcaatca tgccgtgacg gaagagatta
     3061 aatactgggc gcgctgggtg atggaacaaa cgcaatgcga cggctttcgt cttgatgcgg
     3121 tcaaacatat tccggcatgg ttttataaag agtggatcga acacgtacag gaagttgcgc
     3181 caaagccgct gtttattgtg gctgaatact ggtcgcatga agtcgataaa ctgcaaacgt
     3241 atattgatca ggtggaaggc aaaaccatgc tgtttgatgc gccgctgcag atgaaattcc
     3301 atgaagcatc gcgcatgggt cgcgactacg acatgacgca gatcttcact gggacgctgg
     3361 tggaagccga tccttttcac gccgtaaccc tcgttgccaa ccacgacacc caaccgttgc
     3421 aagccctcga agcaccggtc gaaccgtggt ttaaaccgct ggcgtatgcc ttaattttgt
     3481 tgcgggaaaa tggcgttcct tcggtgttct atccggacct ctacggtgca cattacgaag
     3541 atgtcggtgg tgacgggcaa acctatccga tagatatgcc aataatcgaa cagcttgatg
     3601 agttaattct cgcccgtcag cgtttcgccc acggtgtaca gacattattt ttcgaccatc
     3661 cgaactgcat tgcctttagc cgcagtggca ccgacgaata tcccggctgc gtggtggtca
     3721 tgtcgaacgg agatgatggc gaaaaaacca ttcatctggg agagaattac ggcaataaaa
     3781 cctggcgtga ttttctgggg aaccggcaag agagcgtagt gacagacgaa aacggcgaag
     3841 cgaccttctt ttgcaacggc ggcagcgtca gcgtgtgggt tatcgaagag gtgatttaaa
     3901 tccatccccg gcggcaagcc ggggagattt cattacggca gtggcgtcgg taacggcgct
     3961 ttatccagcg cggcggcaca ctctgccgtc gggcgatcca cacgctccat cgtcatacca
     4021 tcgtactcaa tggtgttacc gtcacgctca acctcgtaca gctcacgttt tacggtaacg
     4081 ttagtgagat catcagacat caatgtcaac ttacccggca cggcaatcac ccgctgccac
     4141 tgacggcaat ccagggtatc gccctcttta gtgacaatca ggctgcctat cgcttccgga
     4201 ctgaccagcg cacgttgcgg ccctttggtc tgccagtagc ccgccagcca gtccggcgcg
     4261 ggggttttca cgacattgtt atagttttcg acttcggcac agcccgccag caacatgagc
     4321 gcgcctgcaa ttgcgagttt tttcatcatt cgtcctgcat gagaagaaaa gaggattgtg
     4381 gcattaaagc tctgatgtcg ccagcctttc caggttcaga gagaaatttg atctaactat
     4441 tatttttgtg aattattacc ccgaaaataa atttgcatgg tgtatgattc gcggctcttg
     4501 tttcaaacct tctgagttca gaggctacat tcatgtcatg gcagcaattc aaacacgcct
     4561 ggttgattaa attctgggca cccatccctg cggtcatcgc ggcgggtatt ctctccactt
     4621 actattttgg cattactggc accttttggg ctgtcacggg tgaatttacc cgttggggcg
     4681 gtcagctcct gcagctgttt ggcgtccatg ctgaagagtg gggttatttt aaaattatcc
     4741 atctggaagg atcgccatta acccgcatcg acggcatgat gatcctcggt atgtttggcg
     4801 gctgctttgc tgctgcgctg tgggccaaca atgtcaaact acgcatgcca cgcagccgta
     4861 tccgcattat gcaggccatc attggcggca ttatcgccgg ttttggcgcg cgtctggcaa
     4921 tgggctgtaa cctggcggcg ttctttaccg gcattcctca gttctcgctg catgcctggt
     4981 tctttgccat cgccactgcc attggttcat ggtttggcgc gcgctttacc ctgctgccca
     5041 tcttccgtat tcccgtgaaa atgcagaaag tttctgccgc ctcaccgctg acgcaaaaac
     5101 cggatcaggc gcggcgtcgt tttcgtctcg ggatgctggt ctttttcggc atgctgggct
     5161 gggcgctgct cacggcgatg aaccaaccaa aactggggct ggcaatgctg tttggcgtcg
     5221 gctttggttt actgattgaa cgtgcgcaaa tctgctttac ttcggcgttc cgcgatatgt
     5281 ggatcaccgg acgtacccat atggcgaaag caatcattat cggtatggcg gtgagtgcca
     5341 tcgggatctt cagttacgta cagttaggcg ttgaacctaa aatcatgtgg gcgggaccaa
     5401 atgcggtaat tggtggttta ctttttggtt ttggcatcgt gctggcaggc ggctgcgaaa
     5461 ccggctggat gtaccgcgcg gtagaaggcc aggtgcacta ctggtgggtc ggtctgggca
     5521 acgtgatcgg ctcaacgatt ctggcgtatt actgggatga tttcgctccg gcgctggcca
     5581 ccgactggga caaaatcaac ctgctgaaaa cttttggtcc gatgggaggc ctgctggtga
     5641 catatttgct gctgtttgct gcgctgatgt tgattatcgg ctgggaaaaa cgcttcttcc
     5701 gccgtgcggc accgcagact gctaaggaga tcgcatgaaa aatatcgttc ctgattaccg
     5761 ccttgatatg gtgggtgaac cctgccctta tccggcagtc gcaacccttg aggcgatgcc
     5821 gcaattaaaa aaaggtgaga tcctggaagt ggtgagcgac tgtccgcagt cgatcaataa
     5881 tattccactg gatgcgcgta atcacggcta cacggtgctg gatattcagc aagacgggcc
     5941 gaccattcgt tatttaattc agaagtaatc cctcattccc gctgtggccc acagcgggaa
     6001 catcgttacc tctgacacat agattttacc cgccccctct cctattcttt tcagtatgat
     6061 ctcaggaggt tgctatgtgt ggacgctttg cccaatccca aacgcgtgag gattaccttg
     6121 cgcttctcgc ggaagatatt gaacgcgata ttccctacga tcccgaaccc attggcagat
     6181 acaatgtcgc gccgggaacc aaagttcttc tgctcagtga acgtgatgaa caccttcatc
     6241 tggatccggt tttctgggga tatgctcccg gatggtggga taaaccgccg ctgattaacg
     6301 cccgcgtaga aactgcctca accagtcgca tgtttaaacc gctctggcaa catggtcggg
     6361 caatctgttt tgccgatggc tggtttgagt ggaaaaaaga aggcgacaaa aaaagcctta
     6421 ttttatctat cgcgctgacg gacaacctgt ttttatggcc gcgataggta gcataccgtt
     6481 tgagcgtggt gacgaagccg aaggattttt gatcgtcact gcggcggcag atcaaggtct
     6541 ggtagatatt catgaccgcc gcccgctggt tctgtcgcca gaagctgcac gggaatggat
     6601 gcggcaagac attggtggta aagaagcctc agaaatcgct accagaagct gtgtgccagc
     6661 aaaccagttc acctggcacc ctgtgtcgcg cgcggtgggt aatgtcaaaa accagggcgc
     6721 ggagttaatt caacctgttt gttgacatcc tccacgccct gaatgacgag gacccctgct
     6781 acgttcaggc tgtcgcctga atcatttcgg tgggttcctg cttcaacgag cggcctgact
     6841 gcaccatctc tccacaggca agaacggcgt gccccgccgc taaaatgtta cgagcgccat
     6901 ttacatcggc gttcgctgta tatccacata cctggcatct gaatttactt tgtgacaggc
     6961 gattttcttt cgctgtatga ccacagcacg cgcaacgctg gcttgtgtac gctggcggaa
     7021 cagcaagcac ctgaccgcca cgccagagct gcttgtactc aagctggcgg cgcatttcat
     7081 accagccctg atccagtatc gaacggttta aacctgattt tgcccggaca ttgcgccccg
     7141 gctggctgac cgtacccgct gctgactttg acatgttgct gaccttcaaa tcctcaatga
     7201 ctatcattac gtggtttttg ctgacggtcg ttgtgacttt gtgaaggtag tccctgcgga
     7261 tatttgcgat acaggaatgc agtcgctgta ttttgcgttt ctgcttctgc cagttgttgc
     7321 tgaatttgac cttgcggctt aactgtcgct gaagtctcgc cagcttcttc tggtttttct
     7381 gaaaactgtt tacaggctca aagactgtgc catctgacag cgaggcaagt ttagccacgc
     7441 cagcatccag cccgaccatt gatgctgaag ggtgaaccgg agttgatact tcactttctg
     7501 tctgaatact gatgtaccac ttaccgcagg actggctgac agtgacattt ttcacaacac
     7561 ccgtgacctg acggctgttc cggtagcgca tccagccaag ttttggcaga aaaatacggc
     7621 tgttttcctg agcgagctta acgccttgcg gatagcggaa tgcatcattc tgtcaccgct
     7681 ttttgaatca ggggaacgcc gaccgcttct ggaaaaagtt tttgcaggcc tgctcagggt
     7741 ctttcagtga ctgttgcaat ggctgtgacg gagcatcttt aatccattgc gtttcagtgg
     7801 catttttcca ctcaaccaat caggaagcca ttttcgcgta agggatgtct ttgttaccgg
     7861 ccttatgatt ctcattctga cgtgccagag tacgactgaa aacgaaacga catgcgcctg
     7921 cgaagcgcct catttcacgc gcctgttgac caccgggtct taaccggaat ttgaatacct
     7981 gtagtcgttt cacagggcaa attatacttt ggtctgtgaa aaaaactgat atccgacgcg
     8041 gaaggcactg tgttttcctg atgcatgctc accgggtctt tgttaccaag taccgacgcc
     8101 agatttttga ccatgacgcg acagaaaaac tacgtactta cttttcaaat gtatgtgctg
     8161 attttgaagc tgaactggtt gaaatggatg gcgaaccaga gcacgtccat ctgttaatta
     8221 actatccccc caaactggcg atatccagtc tggtaaacaa acagcctcaa aggcgtatcg
     8281 ggtaggttac tgcgacggga tcgaccagat attgctgcca ggtattaccg caaagatgtt
     8341 ttgtggcgtc ccggctattt tgccagtagc tgcggaggtg cgccaatatc cgtcatccgc
     8401 caatacattg aacaacagca aacacctggt caggtgaaaa aacgagcctt atatccccgc
     8461 cctgaaggac gggcttttac ggtgcactgg ataagcctgg agattatcta cacctgcatg
     8521 gacatcattg tactatgccc cgtaca
//
LOCUS       DADLSA010000157         8541 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.158, whole genome shotgun sequence.
ACCESSION   DADLSA010000157 DADLSA010000000
VERSION     DADLSA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.158"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..1235
                     /locus_tag="JN691_003489"
     CDS             <1..1235
                     /locus_tag="JN691_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAY7244830.1"
                     /translation="KKIRNIMVVVFLLSLLIPISPINAFFSSSYEVYSEAKEYNMSPH
                     EISDIKSKYTKETVTNDSYSHEIINGNGKNILVIFIEGMSSLVISKELTPNLYEFRKK
                     SISFNNYYNHTAATFRGLRGQLTSSYQMTGGYHKDNSGLGQASKVEIEHKLNTYNYIT
                     KLPIILEENGYHTYFQASNSIDDPLSLMLSTLKFNHIYGREDYNKKSGELTDKESFDL
                     LFKKLNEAKQPFFYGVYTVGTHLGLDSPDVKFYDGRNEYLNHFHQLDHWFGDFISHFN
                     KTSLSKDTIVIITSDHASYPSEDYKKTFKTDNTFFVDQIPLIIWSNGVEFKDINAHGL
                     NSLSLTPTILDILGIKESHNMFIGESLFEKEKNKNELNCTSAIGDLYVKTCNGKMEIE
                     KRKEKTELIKQLQLFGDR"
     gene            complement(1304..2040)
                     /locus_tag="JN691_003490"
                     /pseudo
     CDS             complement(1304..2040)
                     /locus_tag="JN691_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009652796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replicase"
     gene            2109..2381
                     /locus_tag="JN691_003491"
     CDS             2109..2381
                     /locus_tag="JN691_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244831.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMHPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            2362..3346
                     /locus_tag="JN691_003492"
                     /pseudo
     CDS             2362..3346
                     /locus_tag="JN691_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3761..3943
                     /locus_tag="JN691_003493"
     CDS             3761..3943
                     /locus_tag="JN691_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244832.1"
                     /translation="MRCEMLHRYVRRKCSFRRSSASSLTDSLRSVVRLRRAVSAHAKT
                     DIRLSTESGDNTGKNM"
     gene            3960..4195
                     /locus_tag="JN691_003494"
                     /pseudo
     CDS             3960..4195
                     /locus_tag="JN691_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4220..4354
                     /locus_tag="JN691_003495"
     CDS             4220..4354
                     /locus_tag="JN691_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7244833.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(4599..5708)
                     /gene="fepE"
                     /locus_tag="JN691_003496"
     CDS             complement(4599..5708)
                     /gene="fepE"
                     /locus_tag="JN691_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY7244834.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFCLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKPSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(6128..6496)
                     /locus_tag="JN691_003497"
                     /pseudo
     CDS             complement(6128..6496)
                     /locus_tag="JN691_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7274..7965)
                     /locus_tag="JN691_003498"
                     /pseudo
     CDS             complement(7274..7965)
                     /locus_tag="JN691_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2480 a         1934 c         1852 g         2275 t
ORIGIN      
        1 aaaaaaaaat aagaaatata atggttgttg tttttttatt atcattgtta ataccaatat
       61 ctccaataaa tgcttttttt tcttcatcat atgaagttta ttcggaagca aaagaataca
      121 atatgtcacc acatgaaata agtgatataa agtcaaaata cacaaaagaa acagtaacaa
      181 acgacagtta ctcacatgaa ataataaatg gaaacggaaa aaatatttta gtaatattta
      241 ttgaaggtat gtcttcattg gtaatatcta aggagctaac cccgaatcta tatgaattta
      301 gaaaaaaatc aatatcattt aacaactatt acaaccatac cgcagctaca tttagaggtt
      361 taagaggaca gttaacctca tcatatcaaa tgaccggtgg gtaccataaa gataattctg
      421 gccttggcca agcatctaaa gtagagatcg aacataaact aaatacttac aattacataa
      481 caaaattacc aatcatactt gaggaaaatg gttaccatac atattttcaa gcatctaatt
      541 caatagatga cccattgagt ttaatgctat caactttaaa gtttaatcat atatacggaa
      601 gggaggacta taacaaaaag tcaggtgaac tcacagacaa agagtctttc gatttattat
      661 ttaaaaaatt aaatgaggct aaacaacctt tcttctatgg tgtttatact gtaggtacac
      721 accttggctt agatagccct gatgtaaagt tttatgatgg gagaaatgaa tatttaaatc
      781 actttcatca attagaccat tggtttggtg attttatatc tcattttaat aagacaagct
      841 tatcgaaaga tacaattgtc ataatcacaa gtgaccatgc aagttaccca tcagaagatt
      901 acaaaaaaac attcaaaact gataatactt tttttgttga tcagattcca ttaataatat
      961 ggagtaatgg tgttgagttc aaagacatta atgcacacgg tttaaattca ctatctttaa
     1021 cgccaacaat actagatatt cttgggatca aggagtcaca caacatgttc ataggtgagt
     1081 ctctatttga aaaagaaaaa aataaaaatg aactcaactg tacatctgca ataggtgacc
     1141 tgtatgtgaa aacgtgtaat ggaaaaatgg aaatcgagaa aagaaaagaa aaaacagaat
     1201 taataaaaca attgcaactt tttggtgata gataattaaa aggcggtttt accccatgtt
     1261 gattattagt ccacgtgggg ttagtttaaa atgataataa aacttacttt ttttcattct
     1321 tgagccgcct gtagaatgtg gctcgactaa cacctaaaga ctcccattct ttttttaaag
     1381 ttatatgaat taatattaaa actaccttta ggccgtcctc ctgaattgct tagctttcct
     1441 cctttacccc ctctctttct ttgaatttct gaacggtgcg tattttttac atactcaata
     1501 aaacgatcct ctgaaaaatt attcgctgtc cattttgaga tgctttttga tattccaaga
     1561 acttcatttt cacttaatgg tgatacgaat tgtaaattgt aggcacaggc tcgttcatag
     1621 caggcttgta accactgctc atactcaggc cacccctgac ggatggcacg atatgcccac
     1681 ttgcgggttt tgtcgaacag agtgcagtta cggcctaaac cataatcggc ggcaattttc
     1741 cagtgactgt ggttcggatt cttgcaaatt agccccgaat atcccgtgtc agctcccaat
     1801 tttctacgta gcgcattctc tacgcgcagc tgcgtagcgt aacggtttca tttttccgtc
     1861 aggtgctgtg cgtatagagg tttccagcgc ataaagcagg tgcgcgtgtc cgttttcggg
     1921 gttggtgatg gtcagcgtag gtgcgggagc gttgcggtcg ctccagtcaa tggctgcgcc
     1981 gatgcggtca acatcaaatc ccagccagaa catggcgtga ggctgattga accacggcag
     2041 cctggcggct gcaaatcggg gtgtggcaga atatgagctt tcagagtaac catccagaat
     2101 gagttcccat gtcacacaac ctggagcacc agaaagtcca caccagaatg gtgaaagagg
     2161 ttctgaaagc cgtcgccaga gccaataatc acccttataa gtccgtattt gcagacttta
     2221 tcaccggaca tccctcctgc acggtctgtt tctgggaaac cttccacaaa atgcacccgg
     2281 attcaccgta cgaatacgtc acgttctgcc atacctgccg tcgttttgat ttatacgaaa
     2341 cagaagcgga gatgaaggcg gatgacccga agtggtggtg attttcagcc ccgaccgctg
     2401 aaacgcctgt tcacagccaa ccagtgctgg acatccttcc tggatgcggg cggtctgcgc
     2461 gatatcgagg ttgaggctgt cacaaaaatg ctggcctgcg gcacgcggat actgggcgtg
     2521 aaggaataca actgcgataa acctgactgc ccccacgtca gatacgtcac taactcatgc
     2581 ggcagccgtg cctgcccgtc ctgcggaaaa aaggccacag acctgtggat agcgacacag
     2641 ctgaatcgtc ttcctgactg cgactgggta cacctggtct tcaccctgcc ggacacgctg
     2701 tggccggtgt tcgaaagcaa ccggtggctg ctgaatgacg tgtgccgtct ggcggtgaag
     2761 aatctgctgt atgccgcccg gaaacggggg caggaacccg gtatcttctg cgccatccac
     2821 acgtatggcc gtcgtctcaa ctggcatccg catgtacatg tcgaaaaaaa cggccggcgg
     2881 gaggaatacg gcgcgctacc tgggtcgtta tctgaagaag ccgccgatag cggcctcccg
     2941 gctggcacat tacaacggag gggcgagcct gagcttccgt tacctggacc acaaaacggg
     3001 agaaacggcg acggaaacgc tgacacagcg tgagctggtc gcgaggctga aacagcacat
     3061 cccggagaag ttttttaaga tggtgaggta cttcgggttc cttgccaacc gggtgtgtgg
     3121 agagaagctg ccgcaggtgt accgtgctct ggggatggat aaaccggaac cagtggcgaa
     3181 ggtgtgctat gcacaaatgg tgaagcagtt cctgagtcgt gacccgttcg aatgcgtgct
     3241 gtgtggcggc cggatggtat accgccgggc cattgcgggg ctgaatgtgg aggggctgaa
     3301 gaaaaacgcg cgggatatca gtctgctgag gtatatgccg gcctgacaca ggctaagtgt
     3361 gcccgggaat cggtgcaagt ataaaaaaca gcctgttgcg gtataagaat cgaccatgca
     3421 gagagagaaa acaccggagt ggagggaaaa gcagaaaagc tcacgtggca taaggcgtgg
     3481 acagcggtat cgtctggttt ttcaatttcc tatacgtgaa ctccagaaca gcatccagat
     3541 tcgctttgag gccgaaagtg aaacaggaac ataacgaagc tgacggaggc ggtcacggct
     3601 ttcccgtggg cgttttcgtc agcagacaag cccgtcaggg cgcgtcaggg ggttttagcg
     3661 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     3721 ttatgcggcg gcagagcaga tggtgagaga acagcgacat atgaggtgtg aaatgctgca
     3781 cagatacgta aggagaaaat gcagcttccg gcgctcttcc gcttcctcgc tcactgactc
     3841 gctgcgctcg gtcgttcggc tgcggcgagc ggtgtctgct cacgcaaaga cggatatacg
     3901 gttatccaca gaatcagggg ataacaccgg aaaaaacatg tgagcaaaaa agaggccccg
     3961 aaaaaaggcc gcgccggtgg cgtttttcca taggctccgc ccccctgaca agcatcacaa
     4021 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga cttaaagata ccaggcgttt
     4081 ccccctggtg gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt
     4141 ccgctgttat ggccgcgttc gtctcattcc acgcctgaca ctcagttccg ggtaggcagt
     4201 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4261 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttaacgccat tggcagcggc
     4321 cactggtaaa caggatgtgc gagagatgta ttgagagttc ttgaagtggc agccttactg
     4381 aggctacact agaaggacag atttggtgac atgtctcgca caagactgtt accacggttc
     4441 agaagttccc caacttactg aaccttcgaa aaaccacctc cccaggtggt tttttcgttt
     4501 tctagagtag aagactacgg ctgtaaggga atgatctcaa gaagatcctt tgaacttttt
     4561 ataaaagttg cttataaaat gatgcatcag cacaactttt actcaatcac actcatcttt
     4621 ctggcctcca tggcatgacg taaaagtata tagccacatg caagcatccc tccaatgaca
     4681 gaagaaagaa ttacaattat ggcattactt ggcccatcct ttctgaccgg taacgttggg
     4741 ctcaattgat actgaaacgg cataaaatca acatccccca ctttcgccac aaccaattga
     4801 ttcacaacat attgacgatt tcgcaactcc ccactcaatt ccgaaacatc cttgattgat
     4861 tttttgatgt tcaatttagt tgctatacca tcagaaccga gagccacagg aaaatctggg
     4921 tcatccttca taatctgacc attgctgtat acaggttttt ttattccagc cgcattagca
     4981 acctccagtg aataattgag gcgtcgaata tctgcattca gctggtttct aatttttatc
     5041 tcatccagtg caagaatttc tttttcaaca ttagttttaa cttctagttt atttcttata
     5101 tcttccatca aatcccttac aacaagggaa gaaacataat taatataccc agacaacaca
     5161 tcgtgagctt ctttacttgt tggagcagta aaactcagag tccatgatac atacaatgat
     5221 ggcttgtcgt ttttcttact ggcattatca tcaatcgact tcatattttt actcaactca
     5281 acaattgcac gatgcagttc gagagcgcta atgttattct ttttaataat ctccataaca
     5341 taaggagatg atctcatata ctcattcaac gaattaacgg actgaaattt cttaataaac
     5401 agagcaaatg cgctatcacg attgatatca aaatccactc ccagaacatg aagcttggaa
     5461 agctccttat ttaattcact ccattgaaca gactcagcag gagtaaccac agcagaacta
     5521 gtccattttt gtggcaaaac aaaagaaaca agaaagccaa tacaagcaaa aaatagaatc
     5581 accccaacaa tttttatttt tgctgcccat aaaacgctaa tcaagcaaaa aaggtcaatt
     5641 tcttcattac cattcggcaa catgttgtaa ttagaaaaat gttcgtcaga gacctttgag
     5701 attttcattg aaaaaaacac aatatattaa catatggttt ttttattcta tcatatgtta
     5761 ggaaaaaacc taaaaaccat tcagcagaca tttaacataa cattttattg cgtgcaccgc
     5821 ctcagaggct ctcagggcat ttctgacggg aaaccggcaa aacaggcaac aaaccacccg
     5881 aaaacccgcc agaaacgcgc aggctctgtt tttactgcat gcatgagtat gcacgcaagt
     5941 gcatagcgcg aaaattcacc ccattcagcc agggtaagtc ctgtttccgg cattcttccc
     6001 atccgaaaac ggtgaactgg gacgcaattt attgcgtggc cgtgaagccg taaggcacgg
     6061 taggtaattg caggtatccg acaatttcga gtagaggatc cactatttca ctacttagaa
     6121 ttcaaaatca gaatcctttc ttcctgctaa ggctatctct gaatgactca accactcttt
     6181 gagattcttc ttctgctcac atgaagtcat tcccggacag aaaaaaacca ttgtcaggcc
     6241 cactggcttc agagaaattc gtttctgcag cagacacaga atccacatca gcaagagacg
     6301 gttcatctgt actttttctc aatctgtaaa gagccttatc aaacgcaacc agtgagatat
     6361 tcaggccgta acgttcattc agcacagtca caatctcttc cctacggata ccatttttct
     6421 tccaggtttc tatatcgttc ttggaatccc tgacagccgc aatggctgtt cttggaccag
     6481 ggttaaaatt cgccatatac cgcccataat gtcttaaatt tgtctcataa tagtccaaaa
     6541 ataagattga aacgcaaaaa atgtcttaca attatcttat tgttgtcctt tccggttcat
     6601 gtatccatga accgcctgat gtggaacgct ttttatcgat ctcatgcgca gccaaagata
     6661 gattcattct ttaaaagtca ggttacatta tcccttaaat ggtcatacac catacgcaac
     6721 cgcgattttc atcgcaattg catgaattaa aaggtaaaaa ctgaagtccc cgtttattta
     6781 tgtaatacgc aacgcattac aaccacctgt aattacatga aaataaagaa ataagatata
     6841 ataccggcgt attaccacgt acgcgggtaa gcgaaaaagc tataacgctt cccggccgac
     6901 aggcggccca aaatccaccg gggcgtgcag gttctcttca gtaacctgca ccattaaatc
     6961 aaaaaggtca tattcgccgg gaagctgcgt atcagtcgaa tccagttgtt catcatgttc
     7021 agatggcatg gggtgatatc cagtagctga gaggtggtta gcgggtttcg ggacgcagcc
     7081 ctaaaccagt cacgtagcgc tagcggagtg tatactgact taggcggtag tgtagtatga
     7141 cagattctgt ggttacggca aagtaacagc cctgtctagg agagggctgt tatctgatat
     7201 aggcttgttt ttttctcacc gtttttgcga gcgctttttc ctgccaagag gtaggtaggg
     7261 taatgaatcc aacttactga tagtgtttta tgtttagata atgcccgatg actttgtcat
     7321 gcagctccac cgattttgag aacgacagtg acttccgtcc cggccttgcc agatgctgcc
     7381 tcagattatg ccgctcaatt cgctgcgtat atcgcttgct gataacgtgc agcttttcct
     7441 tcaggcggga ttcataaagc ggccatccgt ccgtcatcaa taccaccacg tcaaaggccg
     7501 atagcaggct cagaagacgc tccagcgtga atattgtgcg ttcaccaaaa acgtgggcca
     7561 cgacagtcct gcgtaccctg tcatacgcgt aaaacagcca gcgctggcgt gatttagccc
     7621 cgacgtagct ccactgttcg tccatttccg cgcagacgat gacgttcctg cccggctgta
     7681 tgcgcgaggt taccgactgc ggcctgagtt ttttaaatgg cggaaaatcg tgttgaggcc
     7741 aacgccaata aagcgggcgg ttgcccggca tccgacgcca ttcatggcca tatcaatgat
     7801 tttctggtgt gtaccgggtt gagaggcggt gtaagtgaac tgtagctgcc atgttttacg
     7861 gcagtgagag cagagatagc gctgatgtcc ggcagtactt ttaccgttac gcacaacgcc
     7921 gtcagtagcg gaacaggagg gacagctgat ggaaatggaa gccaccggag cacctcaaaa
     7981 gcaccgtcat ccactaaatc agtaagttgg aagcatcacc gcactagcag acagttaatt
     8041 aaaaaagtat aatccctctc cggatttatt gaataatgga gaattatgct gttttctatg
     8101 tcttatttag tcaaatgcgc ttatcaatat tgtgataagc tcccgagtct aaacttaact
     8161 tactgatttt aggttgagga agaccggcat ttttgtagat ccacggcata atatctattt
     8221 attcaacaac gccccccaaa aaaaggtttt tataaatgtt tttaatttca atcattcaaa
     8281 tgttctcggt tatgattata atgcttaata taaaaataaa agatgcattt agagcaatta
     8341 ttctgctttt acttctatta ctatccatgg ttatatctat tgaaataagc agcattatat
     8401 caataggtga gtacataccc acacttgcac tagcaaatat atcagagaca aatagtgttg
     8461 gctggaaaat aaaactacta ccttttcttg tgttcttgtc atttatgatt gcatcattga
     8521 ttttgaaaag attgataaaa g
//
LOCUS       DADLSA010000158         8488 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.159, whole genome shotgun sequence.
ACCESSION   DADLSA010000158 DADLSA010000000
VERSION     DADLSA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.159"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(204..950)
                     /gene="cutC"
                     /locus_tag="JN691_003499"
     CDS             complement(204..950)
                     /gene="cutC"
                     /locus_tag="JN691_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAY7244835.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(964..1530)
                     /locus_tag="JN691_003500"
     CDS             complement(964..1530)
                     /locus_tag="JN691_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7244836.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1746..3479
                     /gene="argS"
                     /locus_tag="JN691_003501"
     CDS             1746..3479
                     /gene="argS"
                     /locus_tag="JN691_003501"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAY7244837.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAAYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            3656..4144
                     /locus_tag="JN691_003502"
     CDS             3656..4144
                     /locus_tag="JN691_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HAY7244838.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4262..4654)
                     /locus_tag="JN691_003503"
     CDS             complement(4262..4654)
                     /locus_tag="JN691_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HAY7244839.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4654..6036)
                     /locus_tag="JN691_003504"
     CDS             complement(4654..6036)
                     /locus_tag="JN691_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HAY7244840.1"
                     /translation="MSMGHAAESYTLLTIGDGLVAQIPALVISTAAGGIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVNFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6033..6653)
                     /locus_tag="JN691_003505"
     CDS             complement(6033..6653)
                     /locus_tag="JN691_003505"
                     /inference="COORDINATES: protein motif:HMM:NF012973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244841.1"
                     /translation="MILLILSMMVLPLPAFILDLLFTFNIALSIMVLLVAMFTQRTLE
                     FAAFPTILLFTTLLRLALNVASTRIILMEGHTGAAAAGKVVEAFGHFLVGGNFAIGIV
                     VFVILVIINFMVITKGAGRIAEVGARFVLDGMPGKQMAIDADLNAGLIGEDEAKKRRS
                     EVTQEADFYGSMDGASKFVRGDAIAGILIMVINVVGGLLVGVLQLA"
     gene            complement(6678..7871)
                     /gene="flhB"
                     /locus_tag="JN691_003506"
     CDS             complement(6678..7871)
                     /gene="flhB"
                     /locus_tag="JN691_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HAY7244842.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLVLLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRIFSAQTSAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRICQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVHLLIFRCRKPWILLTRNRPMSNLAAMLRLPA
                     NLKST"
     gene            8107..>8488
                     /locus_tag="JN691_003507"
     CDS             8107..>8488
                     /locus_tag="JN691_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAY7244843.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         2183 a         2276 c         2065 g         1964 t
ORIGIN      
        1 gttttagcaa tttaaatccc ataatcaatg tgaatataaa aaatattttt agcacaacgc
       61 tatgcgcact gtcacccgct caacagatca cggttatcgt tcgtttttta tactgctcag
      121 ggataaaaaa aggcccctgt tgaaattgca ggggcctggt acgagcaagc atcatattgg
      181 gcgacatgat gcaacggtaa aaatcatttg gcctgatggc gttcaatgat tcctttcatt
      241 tcagcaaccg ccgccccgtc tacgatataa cgcgaatact cgtccgcgtg ttcatctgat
      301 gacatggaca atccttgatt acgataacgc atcggtgagg cttgccacgc tcccgcggag
      361 ctatggactt ccagcactcc ggcatcgagg aagtggtgca ggttttctgc acggactcct
      421 gctccagcca taatgattgg agcatcacga tgggcaataa gttccataat ttttgataaa
      481 ccttgcagtg cgtctgattt ttgccctgat gtcagtaccc gggcaatgcc caattccgca
      541 agattattga gtgtatataa agggttagcg cacatatcga aggcgcgatg aaaagtcact
      601 gccagcggac cggcagcagc cattattttt tccattcgtg gcatatcgac attcccgtca
      661 acatcgagaa cgcccgtcac cagtccagga aaacctaatt cgcggaccgt gcgcacatcc
      721 tcaagaatgg cggcaaactc accgtcgctg tagcaaaaat caccaccgcg tgggcgaatt
      781 atcggatgca cagggatcgt cacccgctgg cgcacggatt tcagtacccc caacgacggc
      841 gttaagcccc cctcttttgg ggctgcgcat aattcaactc tgtctgcgcc gttttgctgc
      901 gccgttagtg cacattccat gctgtaacag caaatttcca gtaatgccat ttttgctcct
      961 taattacgct gactgctcgc tggcaacgat ctcttcaatg gaccacggat gaaacttaat
     1021 agttgttttg ccatcggtga ccgccagcgt cggattcggt aatcgctcat gctcaccttt
     1081 tggtgaactg gttttaactg agatccccgg taggctgagt ccttcatcag aaagcaatgc
     1141 cagcgcacgg gcattcagcg tttctggatc acccggcaga acgatttcaa tatgttccca
     1201 accttcgtgt gggtaacgtt tttccccggg ccacggtagc tccacattgg aaaactgcca
     1261 gtgcgcaacc tgtaccggtt catgcaattt aaacagacaa atcggtctgc cattgatcat
     1321 attttctgac aaaagctcgc cacactgttc aaaaccgcga cgccagcgct cagcggtgac
     1381 gttttgatgg caacgcaaag aaatgtgatc ggcagtcagc ggagtgatat ccaaccccag
     1441 acggcgggaa agttcatcta atgcgtgggt aaatcgcggt aaatccgatg caatatcctg
     1501 cagctcgtcg atagattgcc agttcgccat aatcactctt cgtctttcag taaaagcgtt
     1561 aatttaccct gttgccctgt gccaaccaac cgctgatttc acgccgcttc tgatgcaata
     1621 gtgaaaacgg caatacgcca cgcgcacgtt gctgacgaaa acagccattt gcagtatact
     1681 cccgccctaa tttctttaac tggtgcgggc aatttttgct cgcttcatca atgtaaggta
     1741 ttccggtgaa tattcaggct cttctctcag aaaaagtccg tcaggccatg attgcggcag
     1801 gcgcgcctgc ggattgcgaa ccgcagattc gtcagtcagc aaaagttcag ttcggcgact
     1861 atcaggctaa cggcatgatg gcagttgcta aaaaactggg tatggcaccg cgacaattag
     1921 cagagcaggt gctgactcat ctggatctta acggtatcgc cagcaaagtt gagatcgccg
     1981 gtccaggctt tatcaacatt ttccttgatc cggcattcct ggctgaacat gttcagcagg
     2041 cgctggcgtc cgatcgtctc ggtgttgcta cgccagaaaa acagaccatt gtggttgact
     2101 actctgcgcc aaacgtggcg aaagagatgc acgtcggtca cctgcgctct accattattg
     2161 gtgacgcagc agtgcgtact ctggagttcc tcggtcacaa agtgattcgc gcaaaccacg
     2221 tcggcgactg gggcactcag ttcggtatgc tgattgcatg gctggaaaag cagcagcagg
     2281 aaaacgccgg tgaaatggag ctggctgacc ttgaaggttt ctaccgcgat gcgaaaaagc
     2341 attacgacga agatgaagag tttgccgagc gtgcacgtaa ctacgtggta aaactgcaaa
     2401 gcggtgacga atatttccgc gagatgtggc gcaaactggt cgacatcacc atgacgcaga
     2461 accagatcac ttacgatcgt ctcaacgtga cgctgacccg tgatgacgtg atgggcgaaa
     2521 gcctctacaa cccaatgctg ccaggaattg tggcggatct caaagccaaa ggtctggcag
     2581 tagaaagcga aggggcgacc gtcgtattcc ttgatgagtt taaaaacaag gaaggcgaac
     2641 cgatgggcgt gatcattcag aagaaagatg gcggctatct ctacaccacc actgatatcg
     2701 cctgtgcgaa atatcgttat gaaacactgc atgccgatcg cgtgctgtat tacatcgact
     2761 cccgtcagca tcaacacctg atgcaggcat gggcgatcgt ccgtaaagca gcctatgtcc
     2821 cggaatccgt accgctggaa caccacatgt tcggcatgat gctgggtaaa gacggcaaac
     2881 cgttcaaaac ccgcgcgggt ggtacagtga aactggcaga cctgctggat gaagccctgg
     2941 agcgtgcacg tcgtctggtg gcagaaaaga acccggatat gccagctgac gagctggaaa
     3001 aactggctaa cgcggttggt attggtgcgg tgaaatatgc ggatctctcc aaaaaccgca
     3061 ccacggacta catcttcgac tgggacaaca tgctggcgtt tgagggtaat accgcgccat
     3121 acatgcagta tgcttacacg cgtgtattgt ccgtgttccg taaagcagaa attaacgaag
     3181 agcaactggc tgcagctccg gtaatcatcc gtgaagatcg tgaagcgcaa ctggcagctc
     3241 gcctgctgca gtttgaagaa accctcaccg tggttgcccg tgaaggcacg ccgcatgtaa
     3301 tgtgtgctta cctgtacgat ctggccggtc tgttctctgg cttctacgag cactgcccga
     3361 tcctcagcgc agaaaacgaa gaagtgcgta acagccgtct gaaactggca caactgacag
     3421 cgaagacgct gaagctgggt ctggatacgc tgggtattga gactgtagag cgtatgtaat
     3481 cgatttttcg tgagagtgaa gcctgatcag ggttagccga tcaggctttt ttattgccat
     3541 ctaaatgtat tctgaaaacg gacatgccat tgttttctca ctgttgagta agaggccaga
     3601 agcgtaatat ccaggcccag gaaaacgata acggttcaat ttaaggaata ccgcagtgtt
     3661 taaatttctt gtattaacat taggcattat ctcttgccag gcttacgcag aagatacggt
     3721 tatagtaaac gaccatgaca tttcagccat caaagattgt tggcaaaaaa attcagatga
     3781 tgatactgac attaacgtga tcaaatcatg cctgcgacaa gaatacaatc tcgtcgatgc
     3841 gcaattaaat aaagcctatg gtgaagctta tcgttatata gaacaagtgc cacgcacagg
     3901 tgcaaaaaaa cttgatactg aacaacttaa cttgcttaaa aaatcacagc gagcctggct
     3961 ggattttagg gacaaagaat gtgaattaat cctttcaaat gaggatgttc aggatttaag
     4021 tgacccttat tctgaatcag aatggctatc atgtatgatc atacagacca atacgcgaac
     4081 tcgccagttg cagctatacc gtaactctga agatttttat ccaagccctt tgacaagagg
     4141 ataattcaca tctttttggc atgttttgtt gcaagctatt cctgataaat gattgcaaca
     4201 aaacatccag ccttttgact gagttattaa acatactcgc gagcgcgtaa ttttttgtct
     4261 ttcagcgata attcacaatc acttcattcc gttgtacctt tagcggtgga attaaccgcc
     4321 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
     4381 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
     4441 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
     4501 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
     4561 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
     4621 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
     4681 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
     4741 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
     4801 cgcacccagc atctcctgac gggatagcgc ttcctgagtt tgcgccagta aacgatccgc
     4861 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
     4921 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
     4981 ccgtcccaac gccacgcgca cgacggcggt taattcatgt ggatcgcttt ggatgggcgc
     5041 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
     5101 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
     5161 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
     5221 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
     5281 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     5341 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     5401 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     5461 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     5521 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     5581 caacaactca ccatcctgct ggaaattgac catcgggatc agtcgataac ccacttccat
     5641 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     5701 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     5761 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     5821 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     5881 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     5941 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     6001 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     6061 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     6121 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     6181 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     6241 tgcttacccg gcataccatc gagaacaaag cgcgccccca cttctgcgat acgcccggca
     6301 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     6361 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     6421 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     6481 aacaacgtag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     6541 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     6601 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     6661 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcagcc
     6721 agattactca tgggtcggtt tctcgttaat aaaatccagg gcttccggca ccggaagatg
     6781 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     6841 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     6901 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     6961 ccaatttcac gaatacgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     7021 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     7081 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     7141 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     7201 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     7261 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     7321 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     7381 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     7441 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     7501 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     7561 agcgccacca gcaccacgcc gctaatcagc ggtagcagca ccagcatggc ttctctgatc
     7621 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     7681 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     7741 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     7801 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     7861 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     7921 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     7981 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     8041 cctatagaat cgccctccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     8101 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     8161 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     8221 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     8281 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     8341 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     8401 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     8461 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DADLSA010000159         8420 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.160, whole genome shotgun sequence.
ACCESSION   DADLSA010000159 DADLSA010000000
VERSION     DADLSA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.160"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(58..555)
                     /gene="rayT"
                     /locus_tag="JN691_003508"
     CDS             complement(58..555)
                     /gene="rayT"
                     /locus_tag="JN691_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HAY7244844.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(731..1344)
                     /locus_tag="JN691_003509"
                     /pseudo
     CDS             complement(731..1344)
                     /locus_tag="JN691_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            1636..2376
                     /locus_tag="JN691_003510"
     CDS             1636..2376
                     /locus_tag="JN691_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAY7244845.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(2347..3114)
                     /locus_tag="JN691_003511"
     CDS             complement(2347..3114)
                     /locus_tag="JN691_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAY7244846.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(3320..3898)
                     /gene="lpcA"
                     /locus_tag="JN691_003512"
     CDS             complement(3320..3898)
                     /gene="lpcA"
                     /locus_tag="JN691_003512"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAY7244847.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            4138..6582
                     /locus_tag="JN691_003513"
     CDS             4138..6582
                     /locus_tag="JN691_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY7244848.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGVYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(6625..7098)
                     /gene="ivy"
                     /locus_tag="JN691_003514"
     CDS             complement(6625..7098)
                     /gene="ivy"
                     /locus_tag="JN691_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAY7244849.1"
                     /translation="MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSAIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            7252..8022
                     /gene="yafV"
                     /locus_tag="JN691_003515"
     CDS             7252..8022
                     /gene="yafV"
                     /locus_tag="JN691_003515"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAY7244850.1"
                     /translation="MPGLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            8132..8364
                     /locus_tag="JN691_003516"
                     /pseudo
     CDS             8132..8364
                     /locus_tag="JN691_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
BASE COUNT         2035 a         2115 c         2250 g         2020 t
ORIGIN      
        1 gttaaaacac gatttgtagg ccggataagc gtaagcgcat ccggcaccag cgcacagtta
       61 taaaatgata cgctccccgg cacttaaatc cgttatgtct cccgcccaat cgatgggata
      121 taaccctcgc gcgacatcgc gatggaacgt tgagaatggc caatcactca cctgctttac
      181 ccagccatgt ttctctggat ttatataaat ataatcaaca tgatggcgat aatcttttgt
      241 attgcgaatg gcatgctccc aaaaacgcgg ttgccagata tttttcaatc cgcaagcatg
      301 ggtgaactgc tttttaatct cccgccagcg agaggaaaaa tcatcatcgc tttcgggtaa
      361 tgtccatatg caatgcatat gctccggcaa aacgacccag gcgttaattt caaaaggtct
      421 gtctcgctta acgttaataa tggcgttacg gagcgtctgg aactgggtgg tcagaagatg
      481 gcttcgacga ttgcgtaaat ttaccgtgaa aaaccatgtt ccccccttga tgtaataacg
      541 acgatattcc gacatagcga ctcctttttc tcaacggtaa gaggaaaagg aaaggccgac
      601 aatgacgctg tgttatatcg ggaaagcggc tcgcaaaaac gcacagattt gtaggtatga
      661 taagacgcgt cagcgtcgca tcaggcatcc gtgcacaacc gccggatgcg gcgtaaacgc
      721 cttatccggc ctacaaaatc atctcttccg tcaaaatcct gcgcgcgcct aaaaaatggt
      781 cctgccagaa aggttcggct aaccggctta cccgaatggt ttccccggtg cgcagtgact
      841 caataaactg accgtcgccc agatacaccc ccatatggtc ggcaatctcg cggctgtgga
      901 tatggaaaaa tagcaaatct ccccggcgca ggtcgttatt cgccacaatc gttgcccgac
      961 ggtagtggta catctcattg gccgtgcgcg ggagtttcgc ctcaaggatc ttgttgtagg
     1021 cataaaaaac taacccacta caatcgaagc cttgatcggg ccgcgtgccg ccccagacgt
     1081 aaggcttgcc aagctgctgc tccaggcggt gaatcgccac ttcggtgatg ttatgcaggt
     1141 gatcgctgct taaaaagtgg ttgttttccg ccagcgcctg ccagcgtctg tcactcgcct
     1201 tgagcggcgc aggaaaccat tctggatgct gttttatctg ctcccggttg tggtggcgct
     1261 ggcgagtaaa gggagcaaaa cgcataacga aaacacgcct ggatgcaaaa atctgccgag
     1321 caagaacgaa gacatgaaag acataaaccg gctgaatgaa ggtgagaatt ttaaaagacc
     1381 ggaggagtaa acgggattat taattgctta tttgcatatt actattgaga tttcataatc
     1441 ttaggaaata aatgatacat gcgcttttcg gactattgaa aactgcccct gtgctaaatt
     1501 tgtaaaccca gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttcca
     1561 aatccagact attccgattt gggatccatt ttgccgtcct agcgtgtgta accgttttat
     1621 caaggaataa gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc
     1681 gtttcgtttg ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat
     1741 aagcagcagt tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc
     1801 gatctttacg tcaaaatggg cgagcaatat caactgctcg acagttataa aatctgtaaa
     1861 tattccggcg gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt
     1921 tatagcgtcc agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt
     1981 ggattcccca atgcttatga ccgtgcgcat ggttatgaag ggaaatacct gatgatccac
     2041 ggcgattgtg tatccatcgg ctgctacgca atgaccaatc agggtattga tgagatcttc
     2101 cagttcgtta ctggtgcgct ggtgtttggt cagccgagcg tgcaggtgag tatttaccca
     2161 ttccgcatga ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg
     2221 gagcagctga agccgggcta cgactacttt gagcaaaccc gtaagccacc gaccgtttct
     2281 gtggtcaatg gccgttacgt ggtcagcaag ccgttaagcc acgaagtggt gcagccacaa
     2341 ctggcatcaa actacacgct ccccgaggca aaataagcgc cattcgcctg gcataatttt
     2401 ttgccaggtt tcattgcccg tcagcggctg tgtcgcaatc accgtgacca catcattcgg
     2461 tgtggtctgc gagctgaagt cgatttccac atcctgatcc agcaacgttg ccacgccaaa
     2521 cggcgcgcgg cgggtgatcc agtgtaaatt agtcgagcaa tacgccatta catagcgccc
     2581 gtccgaaagc agcatgttga aaacgccctt cgcccgcagt tcatccgcca gtgaggcgat
     2641 atatttaaac actgccgcca tgttgcccgg cgtgcgcggg taacgctgcg ttaatttatg
     2701 cagcagccag caaaaggctt tttcgctgtc ggtttcaccg accgggcgga agttgccggt
     2761 ttccagtgat ttgtagcccg tcagttgtcc gttatgggca taagtccagt tacgccccca
     2821 taactcgcgg gtaaatgggt gagtattttc cagcgccacc tcgccccgat tagcctggcg
     2881 aatatgagcc accaccgaac aggattttat cgggtagtcc tggacaagtt tggcgatggg
     2941 ggaattaaag ctgggttgtg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt
     3001 aatgccccag ccatctttat gtggcccggt tccaccacca cgctgtacaa gtccggtgaa
     3061 actaaagcag atatcggtag ggacgttggc gctcatcccg agcaattcgc acatgggtaa
     3121 cctccacaac agcgggagcg catggacccc ggcgaagatt gatttaagaa ttttcaggtc
     3181 ggatgcggcg taaacgaatt attcggccta cggttcgggt acagatttgt aggcatgata
     3241 agacgcgtca gcatcgcatc aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct
     3301 tatccggcct acgccagact tacttaacca tctctttttc aattaactgg atcaggatat
     3361 ggatcacttt aatgtgaatc tcctgaatgc ggtcggcata accaaagtgc ggaacgcgaa
     3421 tttcgatatc cgccgtgcca gccattttac cgccgtcttt accggtcagg gtgatcactt
     3481 tcatcccctt ctcacgcgcc gctgcgatcg ctttgatcac gtttgcagag ttaccggagg
     3541 tggagatccc cagcagtaca tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa
     3601 tatcattgaa accgaaatca ttaccgacgc aggaaatatg actaacgtca gaaatagcaa
     3661 tcgccgggta gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca
     3721 tagcgtcgca atgggaaccg ccgttgccgc aggaaagcac tttgccaccg gctttaaagc
     3781 tgtctgctaa caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa
     3841 agttagccag cgtttccgcc gcttcgttca gttcgttacg aataagatcc tggtacatga
     3901 ggatatcctt cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc
     3961 gagaagtacg ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag
     4021 caacaatgtg agctttgttg taattatatt gtaaacacat tgctaaatgt ttttacatcc
     4081 actacaacca tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg
     4141 atgattttga gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg
     4201 agcttattta tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgctggt
     4261 ctgtggtcgg cgtgggtact ggtgcctctg accattatcc tcgtgccatt taactttgcg
     4321 cctatgcgta agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg
     4381 ccgatgtcgc gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac
     4441 ttgttccagg gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc
     4501 gctgaagagc aagcgtttct cgacggcccg gtagaagaag cctgccggat ggcgaatgat
     4561 ttccagatta ctcatgagct ggcggatctg ccgccggagt tgtgggcgta ccttaaagag
     4621 catcgcttct tcgcgatgat tatcaaaaaa gagtacggcg gactggagtt ctcagcttat
     4681 gcccagtctc gtgtgctgca aaaactctct ggcgtgagcg ggatcctggc gattaccgtc
     4741 ggcgtgccaa actcattagg cccgggcgaa ctgttgcaac attacggcac tgacgagcag
     4801 aaagatcact atctgccgcg tctggcgcgt ggtcaggaga tcccctgctt tgcactgacc
     4861 agcccggaag cgggttccga tgcgggcgcg attccggaca ccgggattgt ctgcatgggc
     4921 gaatggcagg gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctacattacg
     4981 ctggcaccga ttgcgaccgt gcttgggctg gcgtttaaac tctccgaccc ggaaaaatta
     5041 ctcggcggtg cagaagattt aggcattacc tgtgcgctga tcccaaccac cacgccgggc
     5101 gtggaaattg gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc
     5161 ggtaaagatg tcttcgtgcc gatcgattac atcatcggcg ggccgaaaat ggccgggcaa
     5221 ggctggcgga tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac
     5281 tcaaccggcg gcgtgaaatc ggtagcgctg gcaacgggcg tgtatgctca cattcgccgt
     5341 cagttcaaaa tctctatcgg taagatggaa gggattgaag agccgctggc gcgtattgcc
     5401 gggaacgctt atgtaatgga tgctgcggca tcgctgatta cctacggcat tatgctcggc
     5461 gaaaaacctg ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcag
     5521 tcgattattg atgcgatgga tattaccggc ggtaaaggca ttatgctcgg gcaaagcaac
     5581 ttcctggcgc gtgcttacca gggcgcaccg attgccatca ccgttgaagg ggctaacatt
     5641 ctgacccgca gcatgatgat cttcggacaa ggagcgattc gttgccatcc gtacgtgctg
     5701 gaagagatgg aagcggcgaa gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa
     5761 catatcggtc acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt
     5821 ttaaccagca gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc
     5881 ctgagcgcca acctcgccct gctttctgat gtctcgatgg cagtgctggg cggcagcctg
     5941 aaacgtcgtg agcgtatctc ggcacgtctg ggggatattt taagccagct ttacctcgcc
     6001 tctgccgttc tgaagcgtta tgacgacgaa ggccgtaatg aagccgatct gccgctggtg
     6061 cactggggcg tacaggatgc gctgtatcag gcagaacagg cgatggacga tttactgcaa
     6121 aacttcccga accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt
     6181 cattatctgg caccgtctga caagctggat cataaagtgg cgaagatttt acaagtgccg
     6241 aacgccactc gttcacgtat tggtcgcggt cagtacctga cgccaagcga gcataacccg
     6301 gttggtttac tggaagaggc gttggtggat gtgattgccg ccgacccgat tcatcagcgg
     6361 atctgtaaag agctgggtaa aaatctgcca tttacccgtc tggatgaact ggcgcacaac
     6421 gcgctgacga aggggctgat tgataaagat gaagccgcta ttctggtgaa agcggaagaa
     6481 agccgtctgc gcagtattaa cgttgatgac tttgatccgg aagagctggc gacgaagccg
     6541 gtaaagttgc cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa
     6601 aggctccgtt tctttatccg ctaattattt aaaattaaag ccatccggat ggttttccag
     6661 gctgccggtc aacgccgcga acagcaccgt tttaccatca atcgaaagcg catcgttcac
     6721 attcagccag gtgagtttct cttgcgacgt tttctcatca atagccgaga acagccccgt
     6781 catctgatta gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgcgg
     6841 tttgcacgcg ctcatcacct gatacgtctc atctcccaac gttacggttt gtgcgggagt
     6901 ataagtaccg cctttcatca cccatgcagg tagcttatgc ccttgcacca tctgattaaa
     6961 cgcagctttg gtggtttcgc cctttgcaag gctgctaatg gttaaatcat cctgcgccat
     7021 tgcactggtg gcgatgacca gtgcggcgac tgtcgttatt gccttaaaca tcattcctcc
     7081 cgagcttatc ctgcccatgt tattaacctc ctaaagcctg gcgataacac gataatttgc
     7141 aaatcagata tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc
     7201 ctgataaggt gttaaagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt
     7261 ttgaagatta cgcttttgca gcaaccgttg gtgtgggtgg atggtcctgc caacctgcgc
     7321 cattttgatc gtcaactgga aggtattacc gggcgcgatg tgatcgttct accggagatg
     7381 tttaccagcg gctttgccat ggaagcggca gcttcgtcgc ttgcacaaga tgacgtagtg
     7441 aactggatga cagctaaggc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta
     7501 caaacggagt ctggttcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat
     7561 ttttatgata agcgtcatct gttccgcatg gcagatgagc atctgcatta taaagcgggc
     7621 aatgcgcgag tgattgtgga atggcgcggc tggcgtattt tgccgctggt gtgctacgac
     7681 ttacgttttc cagtgtggtc gcgcaatctc aacgattatg acctcgccct gtacgtcgcc
     7741 aactggccag ctccgcgctc tctgcactgg caggcattgc tgacggcccg cgcgattgag
     7801 aatcaggcgt atgtggcggg atgcaatcgc gtcggcagcg atggcaacgg ctgccattat
     7861 cgcggtgaca gccgggtgat taatccgcaa ggcgagatta tcgctaccgc cgacgcgcat
     7921 aaggcaacgc gtattgatgc ggagctgtcg atggtggcat tgcgggaata tagagaaaag
     7981 tttccggcat ggcgggatgc ggatgagttt aggttgtggt gagtgggggt tgtgtttagg
     8041 gccgggggct gggctagatt acgaaagccc ctaacttaaa cacaatatta cgccagaagt
     8101 caccatttcc aaaacacagc gtcgctgtac cgtgggttat ctgcccaacc gcagcgacac
     8161 gccggtgatc aaaatcagcg ccaaatggct gagaaaagcg ggatttgaga tcggcacggg
     8221 cgttaccgtg aagatctcgg agggatgttt gatcctgctt gggataacaa cgaagtacag
     8281 gaactgcgca gggaacttta tcaggttaaa cagtcagtta aggggatgcg agacgggatg
     8341 tttagtgtgc tgaacgaaag ttaaataata aaaagccgga aagatctgga agaaatttcc
     8401 ggttattatc gttgctatat
//
LOCUS       DADLSA010000160         8320 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.161, whole genome shotgun sequence.
ACCESSION   DADLSA010000160 DADLSA010000000
VERSION     DADLSA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.161"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            1..1631
                     /locus_tag="JN691_003517"
                     /pseudo
     CDS             1..1631
                     /locus_tag="JN691_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1600..3336)
                     /gene="aceK"
                     /locus_tag="JN691_003518"
     CDS             complement(1600..3336)
                     /gene="aceK"
                     /locus_tag="JN691_003518"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HAY7244851.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHCSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3520..4824)
                     /gene="aceA"
                     /locus_tag="JN691_003519"
     CDS             complement(3520..4824)
                     /gene="aceA"
                     /locus_tag="JN691_003519"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HAY7244852.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4854..6455)
                     /gene="aceB"
                     /locus_tag="JN691_003520"
     CDS             complement(4854..6455)
                     /gene="aceB"
                     /locus_tag="JN691_003520"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HAY7244853.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRSVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6724..7653)
                     /gene="metA"
                     /locus_tag="JN691_003521"
     CDS             complement(6724..7653)
                     /gene="metA"
                     /locus_tag="JN691_003521"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAY7244854.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7810..8253
                     /locus_tag="JN691_003522"
     CDS             7810..8253
                     /locus_tag="JN691_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAY7244855.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2100 a         2050 c         2073 g         2097 t
ORIGIN      
        1 ctcactcact tcaacaaaaa atgcaaaaac cagactttgg cgttgatttc cagccgtcct
       61 gaggggcgtt gcgttgctgc ctgcggtgat ttcgggctag ttatgaaagc atattttgac
      121 aagatggaat caaatggcat cagtgttatg gcagccatat tactggtgga taaccatgct
      181 ttgacggtcc ggctaagaat aaagaacaca actgaaggat gtacccatta cgtggtttcg
      241 gtttatgctc ctaatgtaac taacgataaa ataagaatta tgagcgaaag caaagaggat
      301 attaaacact attctctgat ggattttatg aatgtagatt atagcctcct gaaatggtca
      361 aatgatcatg ttattaacca atctgttgca ataattccag cacttccgaa agaacagcta
      421 ttgatgttaa aaggaactgt ggatgaaata acccctccat tatcaccagc aacgatgaat
      481 ttgctaatgg caattggtca gaatcaccaa cttacgcaac tgatgattca gctccagaaa
      541 atgccagaac tacatagaac agaaatgttg actgcctata atagtattaa cctccccggt
      601 ttatatttgg ctataaatta tggcaatgcg gatatcgttg agactatttt caattcattg
      661 tcagaaacag gatatgaagg gttactctcg aaaaaaaatc tcatgcatat tctggaggca
      721 aaagataaaa atggtttttc tggattattt ttagcgatat cacgtaagga taaaaatgtt
      781 gtaacctcga ttctgaacgt cttacctaaa ctggccgcaa cacatcattt agataatgaa
      841 caggtgtata aattcctgag tgccaaaaat agaacgtcca gccatgtttt ataccatgtt
      901 atggcgaatg gtgatgccga catgctgaaa attgttttgg tcgcgttacc tttgttaatt
      961 cgcacatgtc atttgactaa agaacaggta ctcgatctcc tgaaggcaaa ggatttttat
     1021 ggttgcccaa gactatacct ggcgatgcaa aatggacata gcgatatcgt gaaagttatt
     1081 ctcgaagcat tgccctgtct ggcccaggaa attaacattt cagcttccga tattgtagat
     1141 cttctgaccg ctaaaagtct tgcgcgcgac acgggtttgt ttatggccat gcagcgcgga
     1201 catatgaacg ttattaatac tatttttaac gcattaccca ctctttttaa tacgtttaaa
     1261 ttcgataaaa aaatatgaag cccctcctcc tggcaaataa ttctaatgaa tatcccggtt
     1321 tgttttcagc gatacagcat aaacaacaaa acgttgtaga gacggtttat cttgctttat
     1381 ctgaccatgc acgcctgttt ggatttaccg ctgaagatat tatggatttt tggcaacaca
     1441 aagcgccaca aaaatactct gcctttgagt tggcttttga atttggtcac cgggttattg
     1501 ctgaattaat ccttaataca ttaaataaga tggctgaaag ctttggcttt acggataacc
     1561 ctcgatacat tgcggagaaa aattatatgg aagctttact caaaaaagca tctccccata
     1621 ccgtacgcta aatctttgcc tgcgccgata cgcataaaca tcttccacat gcccttcacg
     1681 tatgcggttt tgtagtgcgc gccagtaatc agcgcggaac aggtcggcgt gcatctcttc
     1741 aaacagcgga ccaatacgcg ggtcggcgca tagccagtgg cgaaactctt ccgggaaaac
     1801 atcgcccggc gagacgctgt accacggttc gctggcaagt tcgtcttccg gatagcgcgg
     1861 cggcgggatg tcgcggaaat tcacttccgt catgtagcaa atttcatcgt aatcataaaa
     1921 aaccacacgc ccgtgacggg tgacaccgaa gtttttaaac agcatgtcgc cagggaaaat
     1981 gttagcagcg gcaagctggc gaatagcgtt accgtattct tcaatggcgt cgcgcaactg
     2041 ctgaccttcc acttgttcca gccagatatt gagcggcacc atccgccgct cgatatagag
     2101 atggcgaatt acaatctgct caccaagatc ggtgattttt tccgctgctt cctggagcag
     2161 taattccatt aataccgggg aaatatgccg cttctccagc acaaagtttt caaactcctg
     2221 ggtgtccgcc attcggccca cacggtcgtg ctctttcacc agttgatagc aggcacgaac
     2281 gtgagcggca gacatctctt tctgcggcgc gaacttgtct ttgatgactt tgaatacccg
     2341 atcaaagccc ggcagcgtaa acaccaacat caccattcca cgaatacccg gcgcttcaat
     2401 gaactgctca ttacagccct gtagataaac gagatattcg cggtagcttt cggttttggc
     2461 gtgcttctgg cagccgatag ccatatacaa ttcagcagtg gttttacctg gcagaatttc
     2521 ccgtagccac tcgaccagcg ctgcgggcag cggtgcataa accataaaat aagaacgcgc
     2581 aaagccaaaa acaatgctcg cttcggcggt cgtcgtcagg caggtatcaa taaataactc
     2641 gccgtcgtcc gtctggtgga tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag
     2701 tttgcccacc agccaggcgg ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg
     2761 actttccgcg aggttgtctg tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat
     2821 gtcacggctt ttattctgcc agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag
     2881 tagagattcc cagccgtgat cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg
     2941 aaagcggcgc tctggctgag agctaaagat aaaaagccgc tcgggagtaa gcgagcagtg
     3001 gtcaaataac cgacagtaca cggagttaaa aaagctctcc gcaatctcga agcgcgggta
     3061 atccggcaac agccgcgtgt aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact
     3121 ttggccgtta gtaatgcagc gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag
     3181 atggatacgg tttttcatcg cctgctggac agcatgccag tcggcctgtt cgaaacgctg
     3241 ctgcgccccg gaagtcactt cgaggaatcg accatactga gcgtcgaagc cttgcaaaat
     3301 ggtttgagca atcagtaatt ccaggccacg cggcatttag cgccctcatc aggagcagag
     3361 aattgccgga tgcggcgtaa acgccttatc cagcctacgt tcggcaacgg ttgtaggcat
     3421 gataagacgc gcaagcgtcg catcaggcat cgtgcaccga ttgccggatg cggcgtaaat
     3481 gccttatccg gcctacagtc agcaacgttt gtaggttgct tagaactgcg attcttcagt
     3541 ggagccagtc agcgcggtga ctgaagacgt gccgccctga ataatagtcg tcactttatc
     3601 gaagtaacct gtacccactt cctgctggtg agatacgaag gtatagccat ctttcgcggc
     3661 ggcaaattcc ggctgctgca ctttctcaac atagtgcttc ataccttcgc cctgcgcata
     3721 agcgtttgcc aggtcgaaca tgttaaacca catgctgtgg atacctgcca gggtgatgaa
     3781 ctggaacttg tagcccatat ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc
     3841 gaggtttttc tgccagttga acgacggcga gcagttataa gccagcagtt tgcccggata
     3901 tttcgcgtgg atagcttgtg caaagcgacg cgccagttcc agatccggcg tggaggtttc
     3961 acaccagact aggtcagcat atggcgcata cgccaggcca cggctgatcg cttgctcaat
     4021 gcccgcatga gtacggaaga agccttcact ggtacgctcg ccggtaataa attcgctgtc
     4081 atacgggtcg caatctgagg tgatcagatc cgccgcatca gcatcggtac gtgcaaccag
     4141 cagggttgga acgcccgtca cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc
     4201 ttcctgagtt ggcactaaaa ctttgccgcc catgtgaccg catttcttca ctgacgccag
     4261 ctgatcttcg aagtgaactg ccgcagcacc ggcttcaatc atcgctttca tcagttcaaa
     4321 ggcattcagg acaccgccaa aaccggcttc cgcatcggca acgatcggca ggaagtaatc
     4381 gacatagcgc ggatcgcccg gctcaatgcc cacggaccat tggatctgat cggcacgacg
     4441 gaaggtgttg ttgatccgct ccaccacagc tggcaccgag tttgccggat agagcgactg
     4501 atccggatac atgctggccg ccaggttagc gtccgccgct acctgccatc ccgacagata
     4561 gactgcttca atacccgctt tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag
     4621 gctgttgatg tagccttttt tcgactcacc gtgcagcaga cgccacattt tcgctgcgcc
     4681 cagttgcgcc agcgtgcatt caggattgac tgaaccgcgt aatttcacca catcttccgc
     4741 actgtatggg cgagtaatgc cttcccaacg cggttgagtc cactctttct gtaattcttc
     4801 aatttgttgt gtacgggttt tcatgtgcag atgctccata gttatgtggt ggtttacgct
     4861 aacaggcggt agcctggcag ggtcaggaaa tcaattaact catcggaagt ggtgatctgt
     4921 tccatcaagc gtgcggcatc gtcaaaacgg ccctgggaga aacgttcttc gcccagttcg
     4981 ctggcaatga ctttcatctc ttcgcccagc atctggcgga acaaggcttt ggtcaccggt
     5041 ttgccattgc tcaacgtttt ttgatgatgg atccactgcc agatcgaggt acgggaaatt
     5101 tcagccgtcg ccgcatcttc catcaggcca taaatcggca cgcagccgtt gccggagatc
     5161 cacgcttcga tgtactgcac agccacgcga atgttggcgc gcataccttc ttcggtgcgt
     5221 tcaccatcac aaggtgccag cagctgatcg gcagtaatcg gcgcgtcttg ttcgcgcaat
     5281 acttcaagct gatttttacg ggagccgaga atgtcgttga ataccgccat tgccgtgtcc
     5341 gcaaggcctg ggtgagcgat ccatgtgcca tcgtgaccgt tattggcttc cagcgcttta
     5401 tccgctttta ctttgttgag cacctggtta ttacgctctt catctttgct cggaataaac
     5461 gctgccatgc cgcccatcgc aaaagcaccg cgtttatggc aggttttaat cagcaggcgt
     5521 gagtaagcat tcaggaatgg tttatccatc gtcacggcct gtctgtctgg caggacacga
     5581 tcgggatagt ttttcaacgt tttgatatag ctgaagatgt aatcccagcg accgcagttc
     5641 agaccaacaa tatggtcgcg cagcgcgtga aggatttcgt ccatctggaa cacggcgggc
     5701 agcgtttcaa tcagcaacgt cgccttgatg gtgccgcgcg gcagattaaa gcgatcttct
     5761 gcatagctga agacttcgct ccaccaggcc gcttcctgcc aggactgggt tttcggcaga
     5821 tagaaatagg gaccgctgcc ctttgccaac agtgcctgat agttgtggaa gaaatagagc
     5881 gcaaaatcaa acaggctacc ggggattgcc tcaccacgcc aggtgacatg tttttccggc
     5941 aagtgcagac cgcgtacccg acaaatcaaa accgctggat tgggcttgag ctgataaatt
     6001 ttgcctgctt cattggtata gctgatggta ccgttaaccg catcgcgcag gttaatttgc
     6061 ccgtcgatca ctttgttcca gtctggtgcc agtgaatctt cgaaatcggc cataaagact
     6121 ttcacattgg cgttcagtgc gttgatcacc atcttgcgct ctaccgggcc agttatctct
     6181 acgctgcggt cttctaagtc cgcaggaatc ccgcggattt tccaatcagc atcgcgaatg
     6241 gaagccgttt ccgaaataaa atcaggcaac gttccgttat caatatcttg ctgctgctga
     6301 atgcgcgctg ccagaagttt attgcgttgt ggcgtaaaat gcgtcaccag ctcagtcaga
     6361 aattctaccg cttcggcagt aagaatttgc ttctcctgct cgccatacgg ccttgtgaaa
     6421 gccagttcat cggttgttgt tgcctgttca gtcatcgtgc agctcctcgt catggatccg
     6481 aaaacttccc ctctgtgaac gaaggaacgt tgtgcgcttt tcgttcagca caactaagac
     6541 tactcattta aaatgcaaaa tcaaaaacaa tttccatttt aattaaaaat acttgataac
     6601 ttattgataa caatatgaat aaaatttatt cacttgctga ggtgcgtttc ggaaataaaa
     6661 aaggcacccg aaggtgcctg aggtaaggtg ctgaatcgct taacgatcga ctatcacaga
     6721 agattaatcc agcgttggat tcatgtgccg tagatcgtat ggcgtgatct gatagacgta
     6781 atagttgagc cagttggtaa acagcaaatt accgtgacta cgccagctcg ctcgcggttt
     6841 gttttgcgga tcgttgtgcg ggaaatagtt atacggtaca tccgggtcta gtccggcttc
     6901 cacatcgcgg aaatattcct gcgccagcgt ttgcgcatca tattcgggat ggcccgtcac
     6961 aaaggcaatg cgcttatctt tgctagcaaa cagatatgca tccccttctt ccgtctctgc
     7021 cagaatttcc agatcggtgt aatcacgaat caacgctgcc ggaaagtcag catagcgcga
     7081 atgcggtgcc agaaatgaat catcaaagcc acgcgtcaga agcgcatgag gatggagaat
     7141 atgatgctcg taaacgccag agagtttgtc ggtgcgagtt tgcttaggaa tgccgtagag
     7201 gatattgagc gcggcctgta ccgcccagca gacaaacagc gtcgaggtga cgtgatcttt
     7261 cgaccactcc agcacctgtt tgatctgcgg ccagtaagcg acatcattaa actccaccag
     7321 gcccagcggc gcaccggtta caatcagacc gtcaaagttc tgttcctgaa tatcttcaaa
     7381 gttacagtag aagttgttca gatgctctgc gggcgtgttg cgcgattcac gggaatcgat
     7441 gcgcaacagc tgaatatcga cctgcaaagg tgagtttgaa agcaggcgca gaaactgatt
     7501 ttcagtttca atcttcttcg gcatcaggtt aaggatcaga accttaagtg gacgaatttc
     7561 ctgaccagac gcacgagaag ttgtcatcac aaagacgttt tcttcacgca agaaattgac
     7621 ggcgggtagc tcgtccggca cacgaatcgg cataacctga ttacctcact acatacgctt
     7681 atacgtttag acatccagat agctgaagat aaccagaaaa tttgccaatg tcgagcctgc
     7741 atgttgaagg tgagaaagtt tcaaggaaaa ggctacgtta gaatataaga atgacgaaaa
     7801 ggagagaaaa tggttattag tattcgccgc tcacggcatg aggaagggga ggaactcgtt
     7861 gcgatttggt gtcgatctgt cgatgccacc cacgattttt tatcagcaga gtatcgggcc
     7921 gagctggaag agctggtgcg ttcctttctg ccggaagcac cgttgtgggt cgcggttaat
     7981 gaacgggagc agccggttgg gtttatgttg ttaagtgggc agcatatgga tgcgctgttt
     8041 atcgatcctg atgtgtgcgg ctgcggcgtg ggtcggatgc tggtggagca tgcgctctcg
     8101 atggcaccgg aactgacaac caacgttaat gagcaaaatg agcaggcggt tgggttctat
     8161 aagaaggtgg gttttaaggt tacgggacgc tctgaggtgg acgatttggg gaaaccgtat
     8221 ccgttgctga atctggcgta tgtgggggaa taagccgccc ctgccgtgcc tgcccaagct
     8281 ggagaagacg cgccagcgtc gcacccggca tttttttcac
//
LOCUS       DADLSA010000161         8167 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.162, whole genome shotgun sequence.
ACCESSION   DADLSA010000161 DADLSA010000000
VERSION     DADLSA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.162"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..1312
                     /locus_tag="JN691_003523"
     CDS             <1..1312
                     /locus_tag="JN691_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7244856.1"
                     /translation="DKVKYPTGVENHGGTLRIWFNFKGKRVRENLGVPDTAKNRKIAG
                     ELRTSVCFAIRTGNFDYAAQFPDSPNLKAFGVNKKEITVKELEEKWLDLKRMEISANA
                     FNRYESIARMMVPKIGGNRLVSAVTKEELLYIRKDLLTGYQNPTKGKAPVKGRSVVTV
                     NYYMTTIAGMFQFAADHGYIEANPFEGIKPLKRARAEPDPLTRDEFIRLIDACRHQQT
                     KNLWSLAVYTGMRHGELVSLAWEDIDLKAGTITIRRNYTKLGEFTLPKTEASTNRVVH
                     LIQPAISVLKNQAEMTRLGKQHNIKVQLREYGRSVNHECTFVFNPQVVRKSKQVGFIY
                     KVDSIGDSWETAIKRAGIRHRKAYQSRHTYACWSLSAGANPSFIASQMGHASAQMVFN
                     VYGAWMTDSNAEQIAMLNQKLADYVPMMSHGHQSDTRGLLKSVS"
     gene            1294..2064
                     /locus_tag="JN691_003524"
     CDS             1294..2064
                     /locus_tag="JN691_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAY7244857.1"
                     /translation="MKISELAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPAEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            2117..2512
                     /locus_tag="JN691_003525"
     CDS             2117..2512
                     /locus_tag="JN691_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244858.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2553..3296
                     /gene="cmoA"
                     /locus_tag="JN691_003526"
     CDS             2553..3296
                     /gene="cmoA"
                     /locus_tag="JN691_003526"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAY7244859.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3293..4264
                     /gene="cmoB"
                     /locus_tag="JN691_003527"
     CDS             3293..4264
                     /gene="cmoB"
                     /locus_tag="JN691_003527"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAY7244860.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKEQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4429..6858)
                     /locus_tag="JN691_003528"
     CDS             complement(4429..6858)
                     /locus_tag="JN691_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY7244861.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNTL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     MYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEACNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFCADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYTELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKSAVQA"
     gene            complement(6883..7983)
                     /locus_tag="JN691_003529"
     CDS             complement(6883..7983)
                     /locus_tag="JN691_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAY7244862.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         1990 a         2068 c         1945 g         2164 t
ORIGIN      
        1 ggataaagtc aaatatccaa caggcgtcga aaaccacggt ggcacattac gcatctggtt
       61 taattttaaa ggtaaacgtg tcagggaaaa tcttggtgtc cctgacactg ccaagaacag
      121 gaagatcgcc ggagagctgc ggacatctgt atgttttgcc attcgtacag gaaattttga
      181 ttatgctgcg cagttccctg actctcctaa cctcaaggct tttggggtaa ataaaaaaga
      241 aattacggtg aaagaacttg aagaaaaatg gctggatctg aaacgaatgg aaatctctgc
      301 aaatgcattc aatcgctatg aatccattgc aaggatgatg gttccgaaga ttggtggcaa
      361 ccgactggta tcagcagtga ccaaagagga attgctgtat atcaggaaag atttgctgac
      421 cggataccaa aatccaacaa aaggtaaagc tccagtaaaa ggacgaagcg ttgtcacagt
      481 aaattattac atgacaacaa tcgctggaat gtttcagttt gctgcagatc acggttacat
      541 agaagcaaat cccttcgagg gaattaagcc tcttaaaaga gccagggcag aaccagatcc
      601 gctaactcgt gacgaattta ttcgcctgat agatgcttgc cggcatcagc agacaaaaaa
      661 cctgtggtca ttggctgtgt acacaggaat gcgtcatggt gaactggtct ccctggcctg
      721 ggaagatatc gatctgaaag caggaacaat tactatcagg cgcaattata cgaaactcgg
      781 tgagttcact ctacctaaaa ctgaagcaag tacaaacagg gttgtgcatc ttatccagcc
      841 cgctatcagt gtcctgaaaa atcaggctga aatgacaaga ctgggtaagc agcacaacat
      901 caaggttcaa ctacgtgaat atggacgttc agtaaatcat gaatgtactt tcgtatttaa
      961 cccccaggtg gttagaaaaa gcaaacaggt aggttttatc tacaaggtag attccattgg
     1021 cgactcatgg gaaacagcca ttaaacgtgc aggcatcagg catcgaaagg cataccagtc
     1081 acgacacact tatgcgtgct ggtcattatc tgccggagca aatccaagct tcattgccag
     1141 ccagatgggg catgcaagtg cccagatggt gttcaatgta tacggagcat ggatgactga
     1201 cagcaatgca gaacagatcg caatgctgaa tcagaagctg gcagattatg tcccaatgat
     1261 gtcccatggt caccaaagtg acacaagagg cttattaaaa tcagtgagtt agcttttaat
     1321 acccgtcatg ttaactgcgt ggagggcaac accacgcttt acgccctgcc gaaacccgag
     1381 gttgtcctgc gctggcgtga acagaccaca gatgacttcc gcttctgttt taagtttccg
     1441 gcgaccattt cgcatcaggc agcattacga cattgcgatg atttagtgac tgaatttttg
     1501 acccgcatgt caccgttggc tccgcgcatt gggcaatact ggctgcaact gcctgccaca
     1561 ttcggcccac gggagctgcc tgcgctttgg cattttctcg attctctccc tgccgaattt
     1621 aattatggtg tggaagtccg ccatccacag tttttcgcca aaggggaaga ggaacaaacg
     1681 cttaatcgcg gtttacatca gcgcggcgtt aatcgggtga ttttagacag ccgcccggtt
     1741 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
     1801 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
     1861 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
     1921 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
     1981 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
     2041 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
     2101 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
     2161 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
     2221 tggttttagc gaactgcaaa gcgctattcg cattcatggt aacgcggtgg aatatattcc
     2281 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
     2341 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
     2401 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
     2461 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
     2521 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     2581 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtagctgaa gtcttcccgg
     2641 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     2701 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     2761 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     2821 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     2881 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     2941 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     3001 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     3061 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     3121 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     3181 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     3241 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     3301 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     3361 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     3421 caacgcggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     3481 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     3541 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     3601 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     3661 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     3721 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     3781 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     3841 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     3901 ttcgccgctg gagcatctct ggcagttgaa agaacaactg gtgaatgagg gtgaactggt
     3961 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     4021 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     4081 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     4141 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     4201 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     4261 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     4321 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     4381 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     4441 agcagactta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     4501 cagcgcagag ttaccggcac aggcatttgc cagctgcgag ctggggatat ccgccgttaa
     4561 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     4621 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     4681 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     4741 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgtata
     4801 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     4861 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     4921 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     4981 cgcatttttc accggatcgg cgcagaaatc accataacga acgtactgtt cgttcttctc
     5041 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     5101 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     5161 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     5221 ggcaaacacg tcaaaatcgt tacacgcttc aaattgcgga gcaacagcct gcttcatcgg
     5281 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     5341 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     5401 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     5461 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacattt gttctttacc
     5521 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     5581 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     5641 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     5701 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     5761 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     5821 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     5881 ttccgccgcc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     5941 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     6001 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     6061 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     6121 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     6181 gctccaggca atttttaagg tattcagcgt gttcattccc cacagcacca caacctggct
     6241 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     6301 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     6361 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     6421 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     6481 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     6541 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     6601 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     6661 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     6721 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     6781 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     6841 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     6901 atctttggcg ttgtactgga aataccgcgt taaaatgtcc agttcgtttt cgctcatgct
     6961 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     7021 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     7081 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     7141 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     7201 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     7261 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     7321 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     7381 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     7441 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     7501 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     7561 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     7621 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     7681 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     7741 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     7801 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     7861 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     7921 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     7981 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     8041 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     8101 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     8161 tttatca
//
LOCUS       DADLSA010000162         7814 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.163, whole genome shotgun sequence.
ACCESSION   DADLSA010000162 DADLSA010000000
VERSION     DADLSA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.163"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            179..397
                     /locus_tag="JN691_003530"
     CDS             179..397
                     /locus_tag="JN691_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAY7244863.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            615..857
                     /locus_tag="JN691_003531"
     CDS             615..857
                     /locus_tag="JN691_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244864.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1187..2116)
                     /gene="fdhE"
                     /locus_tag="JN691_003532"
     CDS             complement(1187..2116)
                     /gene="fdhE"
                     /locus_tag="JN691_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAY7244865.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2113..2748)
                     /gene="fdoI"
                     /locus_tag="JN691_003533"
     CDS             complement(2113..2748)
                     /gene="fdoI"
                     /locus_tag="JN691_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY7244866.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTNAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2745..3647)
                     /gene="fdxH"
                     /locus_tag="JN691_003534"
     CDS             complement(2745..3647)
                     /gene="fdxH"
                     /locus_tag="JN691_003534"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY7244867.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3660..6710)
                     /gene="fdnG"
                     /locus_tag="JN691_003535"
     CDS             complement(3660..6710)
                     /gene="fdnG"
                     /locus_tag="JN691_003535"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6123..6125),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY7244868.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTETYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6904..7737
                     /gene="fdhD"
                     /locus_tag="JN691_003536"
     CDS             6904..7737
                     /gene="fdhD"
                     /locus_tag="JN691_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAY7244869.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1808 a         2004 c         2077 g         1925 t
ORIGIN      
        1 tcctgggcga taactaaacc atttttgagt aataaatgaa acaactgtaa cgctcttatt
       61 atttctggaa aacgcctcgc aaaaacaaaa tgacatgcaa aaatccgctt tttttaaatg
      121 cttttccggc gtaattttcg cacatacatt aatgagtaat atatgtaagg tggtggtaat
      181 ggcgatgaat acggtttttc ttcatttatc agaggaggca attaaacggc tgaacaagct
      241 tcggggctgg cgtaaggttt cacgatctgc aattttacgc gaagcggttg agcaatatct
      301 ggagcggcag caatttccgg tgcgtaaagc aaagggaggc aggcaaaagg gggaggtagt
      361 tggtgttgat gatcagtgta aggagcataa ggaatgaaaa aaatttttca ttcttgaata
      421 taaaaaacag atgcccttat tctggtatta atacaaggct gttttacttg aacttataat
      481 aactgcaact gttacatcgt atctggaaaa cgcctcgcaa aaataaaaaa tgatgcgtaa
      541 atgagctttc tttaaagatt cggcaagggt acttttctct ccatagattc aaaaatgata
      601 ttggcgagat atttatgaac tctctggcag gtatagatat gggcagaatt ttactcgatt
      661 tatcgaatga ggtgattaag caacttgatg atctcgaggt gcagcgtaat cttcctcgtg
      721 cggacctatt aagggaagcg gtagatcaat acctgataaa tcaatcgcaa acagcaagaa
      781 ccagtgttcc tggcatctgg caagggtgtg aggaagatgg tgtcgaatat cagcgtaagc
      841 tgcgcgagga atggtaatgg gaaaaaatga tgtcaatcag attgctgaca acgtgcgcgt
      901 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaagtcatg caaattcaat
      961 atattgaagt ttattggtag gcctgataag cgcagcgcat caggcaatca tacgcttgtc
     1021 atcagtgaca tacccataac cttatcgatg tgttggaggg aagaacgagg caaacagaca
     1081 attgcggcat gatcttgaat gggtaacgcg gaatacgaga gatggtgtcg tcacacccta
     1141 tgagctgtaa tccgggcgac ctgcgccacc cggaaaacgt cagagattac tctccttcac
     1201 ccggaaacag gaacgggtta atggaactac gggcgtagcc ttcttgctcc attcgcgcgt
     1261 ccagtaccag agaggcgagg tcatctgcca cggcttcaac tttcggttct ttttcctgat
     1321 aaagaatctt caggtaagtg ccgcagtcat cgcagctttc ggctttaatc gcggcctgtt
     1381 cgtcatccag cgaccagtaa tgcagtttgc cgctctgctc gcagttgctg cattttacgc
     1441 gcactacgtg ccattcggtt tcacacaggt tgcagtgcag gtaacgcaga ccctgagtgg
     1501 tgccaatttg caccatgctg gacaccggca tagagccaca taccgggcaa tattgacgtt
     1561 gttcgccgta ttcagcgcgg gctttgccgg ggatcagatt ggccatctgc gcccagtaga
     1621 gcgacagcgc agcccagata aacggcgctt tatcgctgct gacggacgag aaatcagagg
     1681 caaacagcgc gctggccata tcttccagct cctgagtcga tgccttctcc agattctcaa
     1741 tcactgccag cgccgggccg ctcatttcag gtttcagctc agcaattagc gccatcagca
     1801 gcttttgcca gtgcttatca cgcggcagaa cgtgaatatc cagcgggggc ttgccctgtg
     1861 cgctggcttc tttaatgcgc gcggtcagat ccatctccag cggatggtcg tacagcacca
     1921 cttcctgggc gtgggcgata agcgcagcaa agcgcaggta atcacccagc ggattatttt
     1981 ctgccagctc gcgcagacgc tcggcgcggc ggttgtataa attcttgagc cgagggaaca
     2041 ataacggcgg aatcatatcc gccgtacgtt tctcgctcga acccagctca tcttgcggga
     2101 ttatgcgaat actcattcag ctttcttttc cgttgtcttg cggacctcac ggtaccagcg
     2161 cgggtgatgt ttcttcgccc aggcgttggt aacccatcct tccaccatgg cggtaatcgt
     2221 gcctttcacc caaagggcgg cgtagatatg caccatgata accacaatta acgccactgc
     2281 ggcaaatgaa tgcagcatta acgcgaatcg gatcaccggg attgagaaag caggcgcaaa
     2341 ataaggacgc cagataatca cgccgctcac cagcagcaga accaggaaaa taatcgccgc
     2401 ccagaaaacg catttctgac cgaagttata acgcccggtg tcacctactt cctcgttgac
     2461 gacgatctta cgaatattct tcgcccaaaa gatatcatcc cgattgatta ggttttgatg
     2521 ccagtaacgg aaaaacatga tgatgaacga ggcaaacata accacgccga caaacgggtg
     2581 cagaattcgc gccagctgcg gtgtgcccat gatttgcatc aaccagttga aggacgggaa
     2641 caaaaagccc agcccgctca ccgccgccag gatgaagcag aaggcggtga tccagtggtt
     2701 gatacgttcc ggcgcggtgt agcgcacgat ggtgtcacgt cgtttcattt gcgctcctcg
     2761 tctttctctt cgtgcagatt attctcttcc tcatccgcac ggttcggacc gacacccacg
     2821 tagtggaaga tactggccgc aaaggtagcc gcaaagccaa cagccgcgag cggtttccag
     2881 atgcctttcc agaatttcac ggtttcgctg atttccgggt tctccggcaa gccatgatac
     2941 agatttggct tgtcagcatg gtgcagcacg tacatgacgt gtgtaccacc gacgcctgcc
     3001 ggatcgtaca gacccgcatt gtcgtaaccg cgggttttca gctcagcaac gcgctcgctc
     3061 gccagcgttt tcatcgactc tttcgtaccg aagtgaatcg cgcccgttgg gcaggtcttc
     3121 acgcaggctg gttcttgccc aaccaccacg cggtcaacgc acagcgtaca tttgtagacg
     3181 cggttgtctt ccgggttgag gcgcggaatg tcgaacggac agcccgcaat gcaataaccg
     3241 cagccaatgc actgctcgga ctggaagtcg acgataccgt tggcatactg aataattgca
     3301 ccttccgccg ggcacgcttt caggcagcct ggatcggaac agtgcataca gccgtctttg
     3361 cggatcagcc attccagttt gtcgttctgc tccacttccg agaagcgcat taccgtccac
     3421 gatttggcgc ttaaatcatt ggggttgtcg tacaccccaa tgttattgcc gacggtatcg
     3481 cgaatgtcgt tccactctga acacgccacc tgacaggctt tacagccgat acaggtggta
     3541 acgtcgatga gtttcgccac ttcttcctgg aagtcccgcg cctgaggcgc gggggtcaga
     3601 ccgttagtcg cggaacgacg aatgatatct tgagattgat aagccatatg tcgtctccgt
     3661 tacacctttt ccacattcac aaggaaggac ttaaactccg gcgtctgcgt gttcgcatca
     3721 ccaacgaatg gcgttaaagt attggcaata aagccttttt tagctacgcc ttcatagccc
     3781 cagtgaatcg gaataccgat ggtatcgata tctttgccgt tcgctttcag cgtgcgaata
     3841 cgtttggtca ccaccgcttt ggctttgata tagccacggt tggaggagac tttcacggta
     3901 tcgccctggg caatgccaag tttattcgcc agcgactccc cgatttccac aaactgctct
     3961 ggctgcaaaa tcgcgttcaa cagcgcgtgt ttggtccagt agtggaagtg ctcggtcaga
     4021 cgataggtgg ttccgacata cgggaactta tcggctttac ccaatgcttc ggcgtcgtct
     4081 ttaaagatac gtgcagccgg gttcgagata acgtttggat gcagcgggtt agttcccagc
     4141 ggcgtttcaa acggctcgta gtgttccggg aacggacctt ccgccatctt atcgagggca
     4201 aacagacgcc ccatgccttc ctgctgcatg ataaacggcc cgacgccgct acccggaggc
     4261 gctgcgctgt agtccggaat atcccagccg gtccacttag tgccgtccca tttcagcaac
     4321 tgacgcttcg gatcccacgg gttaccctgc ggatctgcgg aggcgcggtt atacagaatg
     4381 cggcggttaa gcggccatgc ccatgcccag cccagcgtgt taccgaggcc agacggatcg
     4441 gcgttatcac gacgcgccat ctggttgcct tccggcgtcc agctaccggc gaaaatccag
     4501 cagccacagg aggttgtacc gtcatcgcgc aattgggcga acgagctaag ttgttggcct
     4561 ttcttgacga taaccgcacc ggttgccgga tcggtaatat cggccagcgc cttaccgttg
     4621 ctctccatcg ctacttcttc cgacttcggc tcatgcggaa tggcgtagtt ccaggtcatg
     4681 ttcagcacct gatccgggtt cgcgccaccc tgttcggcat acatcttgcg caagcgcagg
     4741 aagataccgg agaggatctc accgtcagtc agcgcaatcc ccggggcgtc cgcacctttc
     4801 cagtgccact gcaaccagcg acctgagtta acgattgaac cgttctcttc tgcgaagcag
     4861 gtcgatggca gacggaacac ttcggtctgg atcttcgacg agtcaacttc gttcagctca
     4921 ccgtggttct gccagaagtt agaggtttca gtgttcagtg ggtcgatggt gacgaggaac
     4981 ttcagtttcg acagacaacc gatcactttg tttttgttcg ggaatgaggc aacagggtta
     5041 aagccctggc agatatagcc attgactttg ccctctttca tcatctcgaa gtattgcagg
     5101 acgtcgtagc ctttatccca cttcggcaac cagtcaaagc cccagctatt ttccgccgtc
     5161 gctttatcac caaagaaggc cttcatcata gagacgaaga atttcgggta gttgccccag
     5221 tagtttacct ggccttccag cagcggtttt ggcgtgttgg cggtaaggta ggtttgcaga
     5281 tcggtctgct tctcgcttgg cagcgtcatg taacctggca gactctgcga cagtagcccc
     5341 aggtccgtca gcccctgaat attggagtga ccgcgcaggg cgttaacgcc gccgccggcc
     5401 atccccatat tgccgagcag cagctggatc atcgccatcg tacgaatgtt ttgcgcacca
     5461 acggagtgtt gcgtccagcc gagggcatac aggaacgagg cagttttatc gtgagcactg
     5521 gtttctgcga tgtattcgca gactttcagg aacgcgtctt ttggcgtacc acagatgttt
     5581 tcaaccacat ctggcgtgta gcgggaaacg tgctgtttca gcaagttcca cacgcagcgc
     5641 ggatgttgca gcgtggtatc gcgtttggcg aagccgtttt cgtccagttc ataagtccag
     5701 gaggatttat cgtacttgcg tttttccgcg tcgtagccgg tgaacaggcc atcttcaaag
     5761 ccgtaatcct cacgcacgat caggctggcg ttggtatagg tttcggtgta ttcgtggttg
     5821 aatttttcat tgttcagcag gtacagcaat acgcctgaca ggaaagcaat gtcagtaccg
     5881 gaacgaatag gggcatagta gtcagctacc gccgccgtac gcgtaaagcg aggatcgatc
     5941 acaatcagct tcgcgccgtt gtgaattttg gcttccatcg cccagcggaa cccgaccggg
     6001 tgagcttcag cggcgttacc gcccatcacc acgacgaggt tggcgttctt gatgtcgacc
     6061 cagtggttgg tcatcgcacc gcgaccaaat gttggagcaa gacttgctac cgttggtccg
     6121 tgtcagacac gcgcctggtt gtcgaccgcg agcataccca gcgcgcggga gaatttttgc
     6181 gttaaatagc cggtttcgtt actcgacgcg gaagcacaca gcatcccggt ggagagccag
     6241 cggttaacag tcacgccttc ggcgttttgc gcaatgtagt tagcatcgcg gtcttctttc
     6301 atcagtttgg cgatgcgatc aaacgcctct tcccaactga tttgctgcca tttatcagaa
     6361 cctggcgcac ggtattccgg aaacttcaga cggctttcgg agtggatgaa atccaccagg
     6421 ccagcgcctt tcggacaaag tgcaccgcgg ttgaccgggt gatccggatc gccttcgata
     6481 tggaagatag atgctttggc gttttttgct ccgtcaccga ggctgtacat caacagccca
     6541 cagcctacgg aacaataggt gcaggtatta cgggtttcgc gggtgcgcag cagtttatac
     6601 tgccgggttt ccgcgagtgc tacgctgggt gcaaaaccca gtgccgctgc cgtggtgcct
     6661 gccataccgc cagcgcagat cttaaagaac tgccttctgc tgacctgcat ggattgctcc
     6721 ttgtttcgac attgtcacgt cccatttaca ttcgcttgct gcgtgtgcag ggagtgggag
     6781 ttatttttct ttgcggaagg ggccgcaaag gtccagaatt ggctcaattt ccctccatct
     6841 aggaagggtt tgtaacagaa taccataatg ttggtgtgtg tgttcttatc tggttaagag
     6901 aaagtgaaaa aaacacagcg aaaagaaatc gaaaatgtga caaatatcac aggtgttcgt
     6961 caaattgagt tatggcggcg tgatgattta caacacccac ggttagatga ggtcgcggaa
     7021 gaagttcccg ttgcgctggt ctacaacggt atttcgcatg tggtgatgat ggcgtcgccc
     7081 aaagaccttg agtactttgc gctcggtttt tcgctttccg aggggataat agaaagtccg
     7141 cgcgatatct tcggcatgga tgtcgttcct tcctgtaatg gtcttgaagt acaaattgag
     7201 ctttccagcc gccgctttat ggggttgaag gagcgccgcc gggcgctggc gggacgtacg
     7261 ggctgtggcg tatgcggcgt ggagcaactt aatgacatcg gaaaaccggt gcagccgcta
     7321 ccgttcaccc agacgtttga tctcaacaaa ctggatgatg cattacgtca tctcaacgat
     7381 tttcagccag tggggcaact gactggttgt actcacgccg ctgcctggat gttgccatct
     7441 ggcgaactgg tcggcgggca tgaagacgtg ggtcgccatg tggcgctgga caaactgtta
     7501 ggccgccggt cacaagaagg ggaaagctgg cagcaaggtg cggtactggt ttccagtcgt
     7561 gccagttatg aaatggtgca aaagtcggcg atgtgcggcg tagagatttt gtttgcggtg
     7621 tctgccgcga ccacgcttgc tgtagaagtg gctgagcgct gtaatctgac actggtaggt
     7681 ttttgtaaac cgggtcgggc aacggtttat acccatccgc agcgtttgag caattaatta
     7741 aaataagtcc tgcgatatat attaaattgc aggacttatt catttcgtga attttattat
     7801 tttatttata aata
//
LOCUS       DADLSA010000163         7786 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.164, whole genome shotgun sequence.
ACCESSION   DADLSA010000163 DADLSA010000000
VERSION     DADLSA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.164"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            111..644
                     /gene="ymdB"
                     /locus_tag="JN691_003537"
     CDS             111..644
                     /gene="ymdB"
                     /locus_tag="JN691_003537"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAY7244870.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWCGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            586..2067
                     /locus_tag="JN691_003538"
     CDS             586..2067
                     /locus_tag="JN691_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAY7244871.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2075..3232)
                     /gene="mdoC"
                     /locus_tag="JN691_003539"
     CDS             complement(2075..3232)
                     /gene="mdoC"
                     /locus_tag="JN691_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAY7244872.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPRLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIDYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3626..5161
                     /gene="mdoG"
                     /locus_tag="JN691_003540"
     CDS             3626..5161
                     /gene="mdoG"
                     /locus_tag="JN691_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAY7244873.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5154..7697
                     /gene="mdoH"
                     /locus_tag="JN691_003541"
     CDS             5154..7697
                     /gene="mdoH"
                     /locus_tag="JN691_003541"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAY7244874.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPLVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1927 a         1857 c         2057 g         1945 t
ORIGIN      
        1 catcgaatat aactaagttt ctgaacaact cacgttgctg agcagaaaaa tgcgatttaa
       61 ccaaaaagcc tgctgtacac ttaagaaaca agaaggtgta aggaggcgtt atgaaaacgc
      121 gtattcatgt tgtgcagggt gatattacca aactggccgt cgatgtgatt gtgaatgcgg
      181 ctaatccgtc attaatggga ggcggcggcg tcgatggggc cattcatcgc gcagcgggtc
      241 cggccctgct ggatgcctgt ttaaaagtca ggcaacagca gggcgattgc cctacggggc
      301 atgccgttat tacgcttgca ggcgatcttc ccgctaaagc cgtagtacac accgtcgggc
      361 cagtctggtg tggtggtgaa caaaacgaag accagctttt gcaggatgcc tatctgaata
      421 gcctacgact ggtggcggca aacagctata cgtcagtggc ttttcctgca atcagtactg
      481 gggtttatgg ttaccctcgt gcggcagcgg ctgaaatcgc agttaaaacc gtttcagaat
      541 ttattacccg tcacgcttta cccgaacagg tatactttgt ctgttatgat gaagaaaacg
      601 cccacctcta cgaaagactc cttacccaac aaggagatga atgatttgcc ccggctggcg
      661 agcgcggtgc tgccactgtg ttcgcaacat cccggtcagt gtggtctttt ccctctggag
      721 aaaagtctgg atgcgtttgc cgcccggtat cgtctggccg aaatggcaga gcatacgctg
      781 gatgttcagt attacatctg gcaggacgat atgtcggggc ggttactgtt ttccgccctg
      841 ttagccgcag caaagcgtgg cgttcgcgtc cgtttgttgc tggacgacaa caatacgccc
      901 ggacttgacg acattttacg cttgcttgac agtcatccac gcattgaagt ccggcttttt
      961 aatcctttct cgtttcgctt gctacgtccg cttggatata tcaccgactt ttcccgtctc
     1021 aatcgccgga tgcacaataa aagtttcact gtcgatggcg tggttaccct agtgggagga
     1081 cgaaatattg gtgatgccta ttttggtgca ggggaagagc cacttttttc ggatttagat
     1141 gtcatggcaa taggccccgt ggtagaggac gttgccgatg atttcgcccg ctactggtat
     1201 tgcaaatcgg tttcaccctt acagcaggtg ctggatgtcc cggaaggtga aatggcggat
     1261 cgcatcgagt tacccgcctc ctggcataac gatgccatga cgcatcgtta tttacgcaaa
     1321 atggaatcca gtccatttat aaatcatctg gttgatggaa cgttgccgct tatctgggcg
     1381 aagacacgtt tattaagtga tgatccggcg aaaggggagg gcaaggcaaa acggcattca
     1441 ctgttaccgc agcgtctgtt cgatatcatg ggctcaccca gtgaacgcat cgatattatc
     1501 tcttcctatt ttgtaccgac acgcgcaggt gtggcgcaac tcttacggat ggtgagaaaa
     1561 ggggtaaaga ttgcgatcct aaccaattct cttgccgcta acgatgttgc tgtcgtccat
     1621 gccggatacg cgcgctggcg caaaaaattg ctccgctatg gcgtggaatt atatgaactc
     1681 aagccgacgc gtgaacaaag cagtacgtta cacgatcgcg gcataaccgg taattccggt
     1741 gccagcctgc atgctaaaac ttttagcatc gatggtaaaa cggtgtttat cggctctttc
     1801 aatttcgatc cgcgttcaac attgctcaat actgaaatgg gcttcgtgat agagagcgaa
     1861 acgctggcac agttaattga taaacgcttt attcagagcc agtacgatgc ggcctggcag
     1921 ctccgtctgg acaggtgggg acggatcaac tgggttgatc gtcatgcaaa gaaagaggtt
     1981 attctcaaaa aagaacccgc caccagtttc tggaagcggg ttatggtcag actggcgtcg
     2041 atattgcccg tggaatggtt attgtaaatg tggcttaacg ggctggtgct ttatcgttct
     2101 cacgcttaac aaccggttta ccagagaaca aaaacttcag taacgggatg cgtaaatgaa
     2161 tttcatacag aattatcgca atccctacta cgaatatcag gccacagaga aaaccaagcc
     2221 agttggaggt gatgtgcggt gtaatgtatg cgccgaaaaa cagcgttaac gggtggtgaa
     2281 ccagatagat aaacagcgat gcgttaacaa aataagtcac ccgcgctgac tggaagttaa
     2341 gcaaacggtg gccaaaggag aagaccacat tcaccatcca caggccgagg accatggtga
     2401 tcaccgactc ggtttcgtac atccaggcat cgccactgcc atagcgctgg ttgagtaaat
     2461 aagcgacaaa cgccaatgct gcagcgaggg tacagccacg agacggcgtg gtaaacaagg
     2521 ctttaagatg agggaaaatg aaagccagtg cgccgaggat aaagaacggt aaataaaata
     2581 gcgtttgcat gacaataaaa ttgaacatgc cattactcag aatgggtgga tacacaataa
     2641 aaatcgttct tcttattacc gcatagtcga tgccgaggca taaaaaaatc accgatagtt
     2701 ttaccatcga gaatttttta ttcgttttat cagaattttc taaattattt ctgatgcgct
     2761 taaatatcca tacacacagc gtcgtcatga ctactaacac cagtaaaaac cacaggtgtg
     2821 atattaattc ccaggccaac gtattatatt tgtcatacaa tgatagccta ggccaacttt
     2881 ccgctttccc tttgacatat tgcagcataa taaattgcgg taatgtcagt agggggatgg
     2941 ctgttaacat cgggatacct acacgttcga cacgtacttt ccaccatttt ttcaagggat
     3001 agcgtaaaaa aagcatgtag gaaaagtatc cggatataac gaaaaatacc tgcatgcgga
     3061 acgagtggat gaagtcatta aaaagggtca gccacaatga cggttcggcg ctattcacat
     3121 gccatgtatg gctcgaatag attaaagaaa tatgaaaagg gatccctaac aacatcagcc
     3181 aggcgcggat ggagtcgagg aaatattcac gttgcgcggg tactggattc atatatggtt
     3241 aactaatctc ggatttttcg tcttatccct gtcgggttat gcctttaggc ttgttgccat
     3301 agcgacaccg acctgaccgc gccaggcgca ggcttcaagg tttttatgca tagcatcatc
     3361 gctaccacta accagaatgg aagcgtctgt aagacggttg ataaataaat ttgctggcaa
     3421 accctacacg aagtcgatgc ttctgtcttt aggagaagca cggaaagtga aaacggttgc
     3481 aatcaggtgc ttaatccatg agccagcgtg ctgaacgata ccgggattca gttgtcggaa
     3541 tggctggtta tccattaaaa tagatcggat cgatataagc acacaaaggg ggaagtgctt
     3601 actaattatg aaacataaac tacaaatgat gaaaatgcgt tggttgagtg ctgcagtaat
     3661 gttaaccctg tatacatctt caagctgggc tttcagtatt gatgatgtcg caaagcaagc
     3721 tcaatcctta gccgggaaag gctatgaggc gcctaaaagc aacttgccct ccgttttccg
     3781 cgacatgaaa tacgcggact atcagcagat ccagtttaat catgacaaag cgtactggaa
     3841 caatctgaag accccattca aactcgagtt ctaccatcag ggtatgtact tcgatacccc
     3901 ggtcaaaata aatgaagtga ctgccaccgc agtcaaacga atcaaataca gcccggatta
     3961 tttcactttc ggcgatgttc agcatgacaa agacacggta aaagaccttg gttttgccgg
     4021 ttttaaagtg ctttacccga tcaacagcaa agataaaaac gatgaaatcg tcagcatgct
     4081 cggggccagc tatttccgcg tgattggtgc aggtcaggtt tatggccttt ctgcccgcgg
     4141 cctagcaatt gataccgctt tgccatcggg tgaagaattt ccacgcttca aagagttctg
     4201 gatcgagcgt ccaaaaccga ctgataaacg tttaaccatt tatgcattgc ttgactcgcc
     4261 gcgtgcgaca ggtgcttaca aattcgtggt tatgccaggg cgtgacacgg ttgtggatgt
     4321 gcagtcgaaa atctatctgc gcgataaagt cggcaaactg ggggttgcac cgttaaccag
     4381 tatgttcctg tttgggccga accaaccgtc tcctgcaaat aactatcgtc cggagttgca
     4441 cgactctaac ggtctgtcta tccatgctgg taatggcgaa tggatctggc gtccgttgaa
     4501 taacccgaaa catttagcgg tcagcagctt ctccatggaa aacccgcaag gctttggtct
     4561 gttgcagcga ggtcgtgatt tctcccgctt tgaagatctc gatgatcgtt acgatctccg
     4621 tccaagcgca tgggtgactc cgaaagggga gtggggcaaa ggcagcgttg agctggtgga
     4681 aattccaacc aacgatgaaa ccaacgataa catcgtcgct tactggacgc cggatcagct
     4741 gccggagccg ggtaaagaga tgaactttaa atacaccatc accttcagcc gtgatgaaga
     4801 caaactgcat gcgccagata acgcatgggt gcaacaaacg cgtcgttcaa cgggggatgt
     4861 gaagcagtcg aacctgattc gccagcctga cggtactatc gcctttgtgg tcgattttac
     4921 cggtgcagag atgaaaaaac tgccagagga taccccggtc acagcgcaaa ccagcattgg
     4981 tgataatggt gagatagttg aaagcacggt gcgctataac ccggttacca aaggctggcg
     5041 tctggtgatg cgtgtgaaag tgaaagatgc caagaaaacc actgaaatgc gtgctgcgct
     5101 ggtgaatgcc gatcagacgt tgagtgaaac ctggagctac cagttacctg ccaatgaata
     5161 agacaactga gtacattgac gcaatgccca tcgccgcaag cgagaaagca gcattgccga
     5221 agactgatat ccgcgccgtt catcaggcgc tggatgccga acaccgcacc tgggcgcggg
     5281 aggatgattc cccgcaaggc tcggtaaagg cgcgtctgga acaagcctgg ccagattcac
     5341 ttgctgatgg acagttaatt aaagacgacg aagggcgcga tcagcttaag gcgatgccag
     5401 aagcaaaacg ctcctcgatg tttcccgacc cgtggcgtac caacccggta ggccgtttct
     5461 gggatcgcct gcgtggacgc gatgtcacgc cgcgctatct ggctcgtttg accaaagaag
     5521 agcaggagag cgagcaaaag tggcgtaccg ttggtaccat ccgccgttac attctgttga
     5581 tcctgacgct cgcgcaaact gttgtcgcga cctggtatat gaagaccatt cttccttatc
     5641 agggttgggc gctgattaat cctatggata tggttggtca ggatttgtgg gtttccttta
     5701 tgcagcttct gccttatatg ctgcaaaccg gtatcctgat cctttttgcg gtactgttct
     5761 gttgggtgtc cgccggattc tggacggcgt tgatgggctt cctgcaactg cttattggtc
     5821 gcgataaata cagtatatct gcgtcaacag ttggcgatga accattaaac ccggagcatc
     5881 gcacggcgtt gatcatgcct atctgtaacg aagacgtgaa ccgtgttttt gctggcttgc
     5941 gtgcaacgtg ggaatcagta aaagccaccg ggaatgccaa acactttgat gtctacattc
     6001 tgagtgacag ttataacccg gatatctgcg tcgcagagca aaaagcctgg atggagctta
     6061 tcgctgaagt cggtggcgaa ggtcagattt tctatcgccg ccgccgtcgc cgcgtgaagc
     6121 gtaaaagcgg taatatcgat gacttctgcc gtcgctgggg cagccagtac agctacatgg
     6181 tggtgctgga tgctgactcg gtaatgaccg gtgattgttt atgcggcctg gtgcgcttga
     6241 tggaagccaa cccgaacgcc gggatcattc agtcgtcgcc gaaagcgtcc ggtatggata
     6301 cgctgtatgc gcgctgtcag cagttcgcga cccgcgtgta tgggccactg tttacagccg
     6361 gtttgcactt ctggcaactt ggcgagtcgc actactgggg acataacgcg attatccgcg
     6421 tgaaaccatt tatcgagcac tgcgcactgg ctccgctgcc gggcgaaggt tcctttgccg
     6481 gttcaatcct gtcacatgac ttcgtagaag cggcgttgat gcgccgtgca ggttgggggg
     6541 tctggattgc ttacgatctc ccgggttctt atgaagaatt gccgcctaac ttgcttgatg
     6601 agctaaaacg tgaccgccgc tggtgccacg gtaacctgat gaacttccgt ctgttcctgg
     6661 ttaagggtat gcacccggtt caccgtgcgg tgttcctgac gggcgtgatg tcttatctct
     6721 ccgctccgct gtggtttatg ttcctcgcgc tctctactgc attgcaggta gtgcatgcgt
     6781 tgaccgaacc gcaatacttc ctgcaaccac ggcagttgtt cccggtgtgg ccgcagtggc
     6841 gtcctgagct ggcgattgca ctttttgctt cgaccatggt gctgttgttc ctgccgaagt
     6901 tattgagcat tttgcttatc tggtgcaaag gaacgaaaga atacggcggc ttctggcgcg
     6961 ttacattatc gttgctgctg gaagtgctgt tttccgtgct gctggctccg gtacgcatgc
     7021 tgttccatac ggtcttcgtt gtcagcgcgt tccttggctg ggaagtggtg tggaattcac
     7081 cgcagcgtga tgatgactcc acttcatggg gcgaagcgtt caaacgccac ggctcacagc
     7141 tgctgttggg gttagtgtgg gctgttggga tggcgtggct ggatctgcgt ttcctgttct
     7201 ggctggcacc gattgtcttc tcgttgatcc tgtcaccgtt tgtttcggtg atttccagcc
     7261 gtgccaccgt tggtctgcgc accaaacgct ggaaactgtt cctgatcccg gaagagtatt
     7321 caccgccgct ggtgctggtt gataccgatc ggttccttga gatgaatcgc caacgctccc
     7381 ttgacgatgg ctttatgcac gcagtgttta acccgtcatt taacgctctg gcaaccgcaa
     7441 tggcgacagc gcgtcaccgc gccagtaagg tgctggaaat cgcccgtgac cgccacgttg
     7501 aacaggcgct taacgagacg ccagagaagc tgaatcgcga tcgtcgcctg gtgctgctaa
     7561 gcgatccggt gacgatggcc cgtctgcatt tccgcgtctg gaattcccct gaaagatatt
     7621 cttcatgggt gagttattac gaagggataa agctcaatcc actggcattg cgtaaaccgg
     7681 atgcggcttc gcaataaaaa cgtagttgcc tgatgcgcta cgcttatcag gcctacatcg
     7741 ttcctgcaat ttattgattt tgcaagattt tgtaggtcgg ataagg
//
LOCUS       DADLSA010000164         7774 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.165, whole genome shotgun sequence.
ACCESSION   DADLSA010000164 DADLSA010000000
VERSION     DADLSA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.165"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(108..1361)
                     /locus_tag="JN691_003542"
     CDS             complement(108..1361)
                     /locus_tag="JN691_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7244875.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFC
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTHYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1468..2388
                     /locus_tag="JN691_003543"
     CDS             1468..2388
                     /locus_tag="JN691_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7244876.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNCSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAELAELAELAKEPFVFFDPHVGTGLYDDI
                     LGLMRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDA
                     VSEMWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            2523..3743
                     /gene="mlc"
                     /locus_tag="JN691_003544"
                     /gene_synonym="dgsA"
     CDS             2523..3743
                     /gene="mlc"
                     /locus_tag="JN691_003544"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAY7244877.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3868..4563
                     /gene="bioD"
                     /locus_tag="JN691_003545"
     CDS             3868..4563
                     /gene="bioD"
                     /locus_tag="JN691_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY7244878.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KDTPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4516..5808)
                     /gene="clcB"
                     /locus_tag="JN691_003546"
     CDS             complement(4516..5808)
                     /gene="clcB"
                     /locus_tag="JN691_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAY7244879.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(5967..6581)
                     /gene="dmsD"
                     /locus_tag="JN691_003547"
     CDS             complement(5967..6581)
                     /gene="dmsD"
                     /locus_tag="JN691_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAY7244880.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6624..7478)
                     /locus_tag="JN691_003548"
     CDS             complement(6624..7478)
                     /locus_tag="JN691_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY7244881.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7480..>7774)
                     /locus_tag="JN691_003549"
     CDS             complement(7480..>7774)
                     /locus_tag="JN691_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244882.1"
                     /translation="CPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEF
                     GPIEELRQKHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
BASE COUNT         2048 a         1988 c         2043 g         1695 t
ORIGIN      
        1 cgctgcggga attggctggc gcttcgtcta ttttaggatg gctattaacg ttggtttgag
       61 caggcaataa gtccggacgg gtatttaccg cagtccggac ttatttttca ggcgtgcaga
      121 cgacgatgca aacgcgtccc gaccagcaga gcaatgacca gcatcagagc aataaatgcg
      181 ccgacgccgt tccagccata gttatgccag aaaacaccac ccagcgtccc ggcaatactc
      241 gaccccagat agtaactgaa cagatacagc gaggaggcct ggcctttagc gcgttttgca
      301 cgggggccga tccagctgct ggctactgag tgggctgcga agaatccggc tgagaagagt
      361 aacattccgg caaagatcag ccacagcgag ctaaataagg tcatcagtaa accaaacagc
      421 ataactcccg tcgaaaacaa catcaccgga ccgcgcccat agtgggtggt catggttccg
      481 gctttgggtg agctccatgt accggtcaaa taagccagcg ataataagcc aaccacggcc
      541 tgactgacat gccaggggga aagcatcaac cgatagccga tgtaattaaa cagcgtgacg
      601 aacgacccca tcagcaaaaa gccttctgcg aacaataacg gtaatccccg gtcacgccag
      661 tgcagacgaa agttgataaa taacgtctta gggcgcagcg aagttgggca aaaatggcgt
      721 gattcaggga ggattttcca gaacatcaac gccgaggcca gcgcgaaaca accgattgcc
      781 gccagagcaa ttcgccagtt gaaaaagtcc gtgaagacac cactaattaa gcgtccgctc
      841 atgccgccaa ttgagttgcc gctgatatac aaccccatcg aaaaggccac gaaactgggg
      901 tggatttcct cgctaagata agtcatgcca acagctgcca cgccacttaa cgaaagccca
      961 atcaaggcgc gcataatcaa aatgccgtgc cagctggtca tcattgtcga aagtaacgta
     1021 caaatggagg ccaacagtag cgccgtgacc atcactggtt tgcgaccaat ggcatcggat
     1081 agcgggccag taaatagcaa accgatagcc aacatcgccg tggaaatgga cagtgaaata
     1141 ctactgttcg cgggcgttaa gccaaactcc tgcgaaagca ccggaaggat aggctgcaca
     1201 caatagagaa gtgcaaaggt tgccagtccg gcagagaaca gcgccagggt gacgcgcata
     1261 aattgcggcg taccgcgttt aataaattga tttggctgag aaatgctttg cttgtcagtg
     1321 tcgcttgccg gagcgccatc aacagttgta gtacggctca cttgaaatcc ttgctaaata
     1381 tgcctgtgga tcaggcttat acatagggta ggaaaatcga attgttctgt ctaatatatt
     1441 aataatctca aataagatgt tttaaatatg aatattgaac ttcgtcatct gcgttacttt
     1501 gttgctgttg cggaagagct gcattttggg cgcgcggctg cccgcctgaa tatttcgcaa
     1561 ccgccgctaa gtcagcagat tcaggcactg gagcaacaaa tcggtgcccg actgctggca
     1621 cgaactaatt gcagtgtatt gctgacggca gcaggaaaac agtttcttgc agatagtcgg
     1681 caaatcctgt ctatggtgga tgacgctgcc gctcgcgctg aaagactgca tcagggtgaa
     1741 gcgggggagt tgcgtattgg ttttacttcg tccgctcctt ttattcgggc ggtgtccgat
     1801 acgttatcgc tgtttcgccg tgattatcct gatgtccatt tacaaacccg cgaaatgaac
     1861 actcgcgagc aaatcgctcc gctcattgaa ggaacgctgg atatgggatt gctgcgtaac
     1921 acagcgttac cggagacgct tgaacacgca gtcatcgtcc atgaaccgct tatggcgatg
     1981 atcccgcacg atcatcccct ggcaaataat ccgagtgtaa cactggctga actggctgaa
     2041 ctggctgaac tggctgaact ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg
     2101 actgggctgt atgacgatat tctcggactg atgcgacgtt accatttgac gcccgtcatc
     2161 actcaggagg tgggcgaggc aatgaccatc atcggtctgg tttccgccgg tctgggtgtt
     2221 tcaattttgc ctgcgtcatt taaacgtgtt cagctcaacg aaatgcgctg ggtgccgatt
     2281 gctgaagagg atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt
     2341 ccagctgcgc gtaactttcg tattcatctg ctgaatgctc tcaggtgagg gaaatttcag
     2401 cgaaaaagcc cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt gacgacacgt
     2461 attgaagtgc ttcaccatag cctacagatt atttcggagc gcgaaaatat agggagtatg
     2521 cggtggttgc tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg
     2581 cggtttatcg cctgattgat cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg
     2641 cgcagctggc tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg
     2701 tgcgagagct ggaaatcaaa gaagcgggga accgtggccg tccggcggtg gggctggtgg
     2761 ttgaaactga agcctggcac tatctttctc tgcgcattag tcgcggggag attttccttg
     2821 ctctgcgcga tctgagcagc aaactggtgg tggaagagtc gcaggaactg gcgttaaaag
     2881 atgacttgcc attgctggat cgtatcattt cccatatcga tcagtttttt atccgccacc
     2941 agaaaaaact tgagcgtcta acttcgattg ccataaccct gccgggaatt attgatacgg
     3001 aaaatggtat tgtacatcgc atgccgttct acgaggatgt aaaagagatg ccgctcggcg
     3061 aggcgctgga gcagcatacc ggcgtaccgg tttatattca gcatgatatc agcgcatgga
     3121 cgatggcaga ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta
     3181 tcgatcacaa cgtgggggtg ggcgtcatta ccgatggtca tctgctacac gcaggcagca
     3241 gtagtctcgt ggaaataggc cacacacagg tcgacccata tggtaaacgc tgttattgcg
     3301 ggaaccacgg ctgcctcgaa accatcgcca gtgtggacag tattcttgag ctggcacagc
     3361 tgcgtctcaa tcaatccatg agctcgatgt tacatgggca accgttaacc gtggactcat
     3421 tgtgtcaggc ggcattgcgc ggcgatctac tggcaaaaga catcattatc ggggtgggcg
     3481 cgcatgtagg gcgcattctt gccatcatgg tgaatttatt taacccacaa aaaatactga
     3541 ttggctcacc gttaagtaaa gcggcagata tcctcttccc ggtcatctca gacagcatcc
     3601 gtcagcaggc ccttcctgcg tatagtcagc acattagcgt tgagagtact cagttttcta
     3661 accagggcac gatggcaggc gctgcactgg taaaagacgc gatgtataac ggttctttgt
     3721 tgattcgtct gttgcagggt taacattttt taactgttct accaaaattt gcgctatctc
     3781 aatttgggcc aggaaagcat aacttagact ttcaaggtta attattttcc tagtttatat
     3841 ttgtgaagca taacggtgga gttagtgatg ctgaagcgtt tctttattac cggtacagac
     3901 acttctgtag ggaaaacggt ggtttcccgc gcattgctac aagcgttagc ctcccaggga
     3961 aaaacggttg cgggatataa acccgtagcg aaggggagca aagatacacc cgaagggctg
     4021 cgtaataaag atgccttggt gttgcagagt gtttcaacca tcgaactgcc ttatgaagca
     4081 gttaatccta tcgcgttaag cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat
     4141 tacaccctca tttcaaacgg cctggcaaac ctgaccgata aagtcgatca tgtcgtggta
     4201 gaagggactg gcggctggcg cagtctgatg aatgatttgc gtccactctc tgaatgggta
     4261 gtgcaggaac aactgccggt gttgatggtt gtcggtattc aggaaggttg cattaaccat
     4321 gcactgctaa cagctcaggc gatcgccaac gacgggctgc cgctcattgg ctgggtggct
     4381 aaccgaatca acccaggact ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa
     4441 cttccggcac cgctcattgg tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg
     4501 gggcaataca tccgcttagc tatgctgcgc agtgtgctgg cggtagatag agtcacggtg
     4561 taacgtccgc gaaattaccg acgcaattac gcaggcaatc aataaaccgg ggagtagctg
     4621 atactccccg gtcatttcac atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc
     4681 cgccaacagt gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg
     4741 gaaccataat cccaggctac gaccatacaa catgccaatg gcaagaccga taaatagcgt
     4801 cggtgtaaag acaccaccgg gtgcgccgga accgctactc gccagcacgg cacacagttt
     4861 acaaaggaag atcccggcaa tgatcattaa cagtggtggg gcggttaaaa aggattgtac
     4921 ggtgctatag ccgttgcccc acactgcagg tgtgaacagg gaaagcagac ccacgatcaa
     4981 cccgcctagt gccagttgcc agggcggcgc aagtttgaga ctcacaaatc cacgatgaca
     5041 ggcgttcatt aacgttaaca acagtggtcc gcacagacct gccagcacac ctgtactgat
     5101 aatcagcgca tagtcacgag cctgaaccgt cactgagagt tgtacgttgt agagtaacgc
     5161 gtcgctatga ttaatcagat tgctaaccag caatgccacg acggcggaaa taatcaccgg
     5221 gccgagagag gccaacatca tagtgccaaa cagcacttcg gctataaata aactgccagc
     5281 aagcggggca cgataggccg cagccattcc cgccgcggcc ccacaggcga tccataattt
     5341 ccattcctgg cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg cagctaaaag
     5401 aatcatcgca ccttcgcgac caattgcact gccgctggtt actaccagca gcgaggcaag
     5461 cgatttaacc aggcttgctg cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta
     5521 atcggtcggc gcatgagggc gttgttgggt aaatttctgc cagcccatca gcaacaaacc
     5581 cgccgccagt ccgccgagcg ccggagttag cagccgtcgc cagggggaaa ggtttgtcgc
     5641 tgcattgacc agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc
     5701 atgacgaaac ccggcaacgg caaagaccgc gagaataccg acgactgttg cgataagcag
     5761 acggcggaac atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg
     5821 gcaatgaaac acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg
     5881 ctttaagaga taaaaaaacg tgacattgta acgtattatt gccgaatcgc gcgtaatcgt
     5941 caccatccgg caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc
     6001 aacagttgtg attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag
     6061 aaagggtgtt ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg
     6121 tgccatgcca gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc
     6181 atcaacagca gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg
     6241 cctttctcgc gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg
     6301 cgatccagcc agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa
     6361 cgctgccagg cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc
     6421 gctaatgacg cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca
     6481 accagaggtg cggcttccgc actctctgga gcgtaataaa acagcgcgcc caacacgcgc
     6541 gccgcgacag aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt
     6601 gcgggttgcc ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg
     6661 ccataaaaga gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca
     6721 agtccggcaa cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct
     6781 gcgcataaca ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg
     6841 gcttgttgca cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg
     6901 gcacaagcga tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg
     6961 cgggctgccc gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag
     7021 aaagccagcg tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag
     7081 gtcatcatcc agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc
     7141 aacgcttgcg gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa
     7201 aatatggaac cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag
     7261 ttaaacgctc gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc
     7321 aacagccaga ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg
     7381 gcgaaccagc caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta
     7441 aagataacca gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc
     7501 cagataacca gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt
     7561 gggttttgtg aaatgcgcgc gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg
     7621 gcgcagctct tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat
     7681 gggttgtttc ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt
     7741 ttcagcattg tactgtggtg cgccgtacgg acaa
//
LOCUS       DADLSA010000165         7735 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.166, whole genome shotgun sequence.
ACCESSION   DADLSA010000165 DADLSA010000000
VERSION     DADLSA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.166"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..518
                     /locus_tag="JN691_003550"
     CDS             <1..518
                     /locus_tag="JN691_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244883.1"
                     /translation="IIDIFLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMEN
                     NHPLCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYI
                     STLGAFAKKHSFSQHQLFTLLAAKNHDNMSAVHIAIHHKHYKTVETYYAAINVISQSL
                     SFSADEIKTYL"
     gene            690..797
                     /locus_tag="JN691_003551"
     CDS             690..797
                     /locus_tag="JN691_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244884.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(850..1608)
                     /locus_tag="JN691_003552"
     CDS             complement(850..1608)
                     /locus_tag="JN691_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HAY7244885.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            1895..2824
                     /gene="pfkB"
                     /locus_tag="JN691_003553"
     CDS             1895..2824
                     /gene="pfkB"
                     /locus_tag="JN691_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAY7244886.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2925..3215
                     /gene="ghoS"
                     /locus_tag="JN691_003554"
     CDS             2925..3215
                     /gene="ghoS"
                     /locus_tag="JN691_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY7244887.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3321..4181
                     /locus_tag="JN691_003555"
     CDS             3321..4181
                     /locus_tag="JN691_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAY7244888.1"
                     /translation="MWQAIIRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4222..4758)
                     /locus_tag="JN691_003556"
     CDS             complement(4222..4758)
                     /locus_tag="JN691_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244889.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4905..5573
                     /gene="hxpB"
                     /locus_tag="JN691_003557"
     CDS             4905..5573
                     /gene="hxpB"
                     /locus_tag="JN691_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HAY7244890.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     EQPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            5736..6326
                     /locus_tag="JN691_003558"
     CDS             5736..6326
                     /locus_tag="JN691_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7244891.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6459..>7735
                     /locus_tag="JN691_003559"
     CDS             6459..>7735
                     /locus_tag="JN691_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAY7244892.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     DSFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRT"
BASE COUNT         1992 a         1862 c         1870 g         2011 t
ORIGIN      
        1 aaataatcga tatctttctg aatgcattaa ccactccaat agcacaagag cttttaaaca
       61 aaaaactggt gatgagtatt ttagccatga aaatacatga tggtgagcca ggattatacg
      121 ccgcaatgga aaataatcac cctttgtgtg tcacacggtt cctctctaaa attaatggca
      181 tcgcctttaa atacaagttg agcaaagcta acatcatgga tttattaaaa ggagctacag
      241 cacagggaac ccctgcatta tacatcgcca tgagcaaggg taatgaagac gtcgtgttat
      301 cttatatatc gacgctgggt gcttttgcaa aaaaacattc ttttagtcaa catcagttat
      361 ttacactatt ggccgctaaa aatcatgaca acatgtcagc tgttcatata gccattcatc
      421 ataagcatta taaaactgta gaaacatatt atgctgctat aaatgtcatc agccaaagcc
      481 tgagttttag tgctgatgaa ataaagacgt atttataacc agctatttac gatatatatc
      541 gatacataat tttatttttt tcataaaacg cagaaacagt gaccatgtgc acaacgccac
      601 ccgacggctg taataacaaa aaatcccttg atgcctgtcc cttttgttac actccgttat
      661 cacgcacaag agatatgcag gacactggta tgccgactaa acgctttgat aaaaaacact
      721 ggaagatggt ggtggtgcta ctggcaatct gtggcgctat gttgttgcta cgttgggcag
      781 caatgatttg gggctgagca gtgacaaacg aagacaacca gaccgcataa cacggtccgg
      841 cagataattt tacatgctgt agcccagcga taaggttgtc cgacggtcgg tatgttccgg
      901 tgcagactct ggaggttcag agttccatgt gacgttgtaa gccaccttca gtccaaaatg
      961 ttcattaata gcaacattta atgcgctttc ggagttcagc gttgtgtctt ccgcgccaaa
     1021 gaccgaaaca ccctgcgtaa atttagcgtt gtcagtcaac tgccatgcat aagccccgga
     1081 ggcatagccc agcggctggg tttcactggc attgtcggta tatttgtcgt aacgcacacc
     1141 tggaccgaat tcaaagcgga aactgtgtac cgggccattc aaaaactgac gaccataacc
     1201 cgcggtcaac acatcgcgct ctcgatagcc gttataacgg tctgtcagcc agcttgcctg
     1261 accaaataaa tagtcataat cagttaaatt aaagcggcta cgcccgcccg ccgcatattt
     1321 ttctgaagaa cgctcatcat tagaagaagt attactggcg ttcccccaca gcgaccaggc
     1381 agtggtttgt ccataccagg tcatggtggt gtcagccgta agtgaggagc ttttcgtatt
     1441 gcctgattgt gcaagatatc ctgcgttcag attaccttcg aaaggttttt tagcgctggc
     1501 aggatcgtcc atgacagtaa aaacggaatc atcggcagct gcattcagtg acgcaaacat
     1561 gccccccgcc aacataacga tggcaggaac tgtcttcaaa agcttcattt atcaagagtc
     1621 cgtacaacaa aaaaagagac catcgcggtc ccggaaactt tcttaaggat caaagattag
     1681 cgtccctgga aaggtaacga attataaaaa ggcgcgaata acttagcaat gtattcttat
     1741 ttcatttttt gaataagcat gtggcgaaaa cagattttta tttatatata tttatctgca
     1801 aaattttaaa taaagctcta ataaatcata ttgttaattt cttcactttc cgctgattcg
     1861 gtgccagact gaaatcagcc tataggagga aatgatggta cgtatctata cgttgacact
     1921 tgcgccctct ctcgatagcg caacaattac cccgcaaatt tatcccgaag gaaagctgcg
     1981 ctgtaccgca ccggtgttcg aacccggggg cggcggcatc aacgtcgccc gcgccattgc
     2041 ccatcttgga ggcagtgcca cagcgatctt cccggcgggt ggcgcgaccg gcgaacacct
     2101 ggtttcactg ttggcggatg aaaatgtccc cgtcgctact gtagaagcca aagactggac
     2161 ccggcagaat ttacacgtac atgtggaagc aagcggtgaa cagtatcgtt ttgttatgcc
     2221 aggcgcggca ttaaatgaag atgagtttcg ccagcttgaa gagcaagttc tggaaattga
     2281 atccggggcc atcctggtca taagcggaag cctgccgcca ggtgtgaagc tggaaaaatt
     2341 aacccaactg atttccgctg cgcaaaacca agggatccgc tgcatcatcg acagttctgg
     2401 cgaagcgtta agtgcagcac tggcgattgg taacatcgag ttggttaagc ctaaccaaaa
     2461 agaactcagt gcgctggtga atcgcgaact cacccagcca gacgatgtcc gcaaagccgc
     2521 gcaggaaatc gttaatagcg gcaaggccaa acgggttgtc gtttcccttg gtccacaagg
     2581 agcgctgggt gttgatagtg aaaactgcat tcaggtggtg ccaccaccgg tgaaaagcca
     2641 gagtaccgtt ggcgctggtg acagcatggt cggcgcgatg acactgaaac tggcagaaaa
     2701 tgcctctctt gaagagatgg tccgttttgg cgtagctgcg gggagtgcag ccacactcaa
     2761 tcagggaaca cgtctgtgct cccatgacga tacgcaaaaa atttacgctt acctttcccg
     2821 ctaacaaaaa cattccccca gcattggggg aatcaccacc aacctgtcgg caacgcgttt
     2881 ctccgactat gctcaaaagt catgtgataa caaaggggtg aactatggcc agtggcgatc
     2941 ttgtccgtta tgtcataacc gtcatgttgc atgaggatac attgactgaa attaacgagt
     3001 tgaataatta cctgactcgc gacggttttt tgctcaccat gacggatgat gagggaaata
     3061 tccatgagct ggggactaac acttttggac ttatcagtac ccaaagtgaa gaagaaatta
     3121 gagaactggt ttcggggctt acccaaagtg caaccggcaa agatcctgaa atcaccatca
     3181 cgacctggga ggaatggaat agcaacagaa aataaatggt ttttgggcaa taatcagtct
     3241 gtggtgtgcg ttagctcgtg tttttacacc gcattcttgc gctaacctta tgatctggca
     3301 gacaacatgg gagagacatc atgtggcagg caatcattcg tcttttgagc gagcagttag
     3361 gtgaaggcga aatcgaactg cgtaatgaac tgcctggcgg agaagtccat gccgcatggc
     3421 atttgcgcta tgcaggacat gactttttcg tcaaatgtga tgaaagggaa ctgcttcccg
     3481 gttttaccgc cgaagccgac caactggagt tactgtcgcg tagtaaaacc gtcaccgtgc
     3541 ctaaggtttg ggcagtaggc gctgaccgtg actacagttt tctggtgatg gattatctcc
     3601 cacctcgtcc gctggatgcg catagcgcat ttattcttgg tcagcaaatt gcgcgtttac
     3661 atcaatggag tgaccaacca caatttggcc tcgattttga taactcgctc tccacaactc
     3721 cacagcccaa cacctggcaa cgtcgctggt caacgttttt tgctgaacaa cggattggct
     3781 ggcagttgga actggcagca gagaaaggga tcgctttcgg caatatcgac gccatcgtcg
     3841 agcatattca gcagcgtctg gcctcgcatc aaccgcagcc ttctctgttg cacggcgatt
     3901 tatggtccgg caactgtgcg ctgggtccgg atggcccgta cattttcgac cctgcctgct
     3961 actggggtga ccgagagtgc gacctagcga tgttaccgct gcatactgaa cagccgccac
     4021 aaatctatga cggctatcag tcagtatccc cgctacctgc cgatttcctt gaacgtcaac
     4081 cggtttacca actctacacg ctgttaaatc gtgcaagatt atttggcggt cagcatttgg
     4141 ttattgctca gcagtcattg gatagattat tagcagcatg atatgggttg aggataatgg
     4201 ccgctccgtg cggccttttg attaaataaa cccaagcaga gaaaagaaca cataacctgc
     4261 agcaatacaa ataaccggca ggatatatag tgagaaaaac tgcaggaaaa tagtatggtg
     4321 cggaacaaca atacgggact caatttgttc acgcgttagc ccctcttccc ctttggcttt
     4381 ttccagaatg agttgatctt caacaccttc ccgcaagaag cgcgcctggc gactcatccg
     4441 ggcaccggaa tcctgcaacg ccagtccgac aaaaatcagg atgaaaatca cccagaacat
     4501 aacgttcaca ccaccattaa aattaggggt cggggagtta taccagaaca gattcaaaaa
     4561 aggcgtattc gcctgcatca tatcgatcat gacatgagca aagtcgagca tgaccgcatt
     4621 aatgccttcc tggttttcct gccgggtatt cataaatttc agcaaagaaa tcaatgtaga
     4681 gatcaatgca ggtataaaaa tcacccaacc caaaatcctt ttcaaaacag caatgcgtcc
     4741 agcttgttga tacgtcatga gttctccttg attaagacgc gtcgtttcac ttagtttacc
     4801 tgtagatatc tgttttcgcc cattctttaa aggcgatatg ataggcgctt aatcataagc
     4861 acggcttaat accttacaca taatgctcta aaggagaggt tgtaatgtca accccgcgtc
     4921 agattcttgc tgcaattttt gatatggatg gattacttat cgactcagaa cctttatggg
     4981 atcgagccga actggatgtg atggcaagcc tgggggtgga tatctcccgt cgtaacgagc
     5041 agccggacac cttaggttta cgcatcgata tggtggtcga tctttggtac gcccggcaac
     5101 cgtggaatgg gccaagccgt caggaagtag tagaacgggt tattgcccgt gccatttcac
     5161 tggttgaaga gacacgtcca ttattaccag gcgtgcgcga agctgttgcg ttatgcaaag
     5221 aacaaggttt attggtggga ctggcctccg cgtcaccact acatatgctg gaaaaagtgt
     5281 tgaccatgtt tgacttacgc gacagtttcg atgccctcgc ctcggctgaa aaactgcctt
     5341 acagcaagcc gcatccgcaa gtatatctcg actgcgcagc aaaactgggc gttgacccac
     5401 tgacctgcgt ggcgctggaa gactcggtaa atggcatgat cgcttctaaa gcagcccgca
     5461 tgcgttccat cgtcgttcct gcgccagaag cgcaaaatga tccacgtttt gtattagcaa
     5521 acgtcaaact ctcatcgctg acagaactca ccgcaaaaga ccttctcggt taatgaccag
     5581 gggcagtgat cacgctgccc ctggttcttt atctgaattt gcattcaact gacggattaa
     5641 tcgtcaattt aagagaaaga gttacaccgt caccacttcc gtgcactgta taaaaatcct
     5701 atactgtacg tatcgacagt ttagtgagtt ttatcatgac ggcggaaggt caccttctct
     5761 tttctattgc ttgtgcggta tttgccaaaa atgccgagct gacgcccgtg ctggcacagg
     5821 gtgactggtg gcatattgtc ccttccgcaa tcctgacgtg tttgttaccg gacatcgatc
     5881 acccaaagtc gtttcttggg cagcgattaa aatggatatc aaaaccgatc gcccgcgctt
     5941 ttgggcatcg tggttttacc cacagtctgc tggcggtatt tgcgctgctg gcaacctttt
     6001 accttaaggt tccggaaggc tggttcattc cggctgatgc gctacaagga atggtgctgg
     6061 gttatttgag ccacatactt gccgatatgc tgacacccgc cggtgttccc ctgctctggc
     6121 catgccgctg gcgtttccgc ttgcctatcc tggttcccca aaagggcaac caactggaac
     6181 gttttatctg catggcatta tttgtctggt cggtatggat gccccattca ttacccgaga
     6241 acagcgctgt tcgttggtca tcgcaaatga tcaatacctt gcagatccag tttcatcggc
     6301 ttattaagca tcaggttgaa tactaaaaag gcaaaaatca cctttctgga ataagcaatt
     6361 ccatttgaat ataagagcca gctcacagtt ctgttaatct tgcgccaaca ctatgactgc
     6421 tacgcagtga tagaaataat aagatcagga gaacggggat gaactttcca ttaattgcga
     6481 acatcgtggt gttcgttgta ctgctgtttg cgctggctca gacccgccat aaacagtgga
     6541 gtctggcgaa aaaagtgctg gtgggtctgg tgatgggtgt ggtttttggc cttgccctgc
     6601 ataccattta tggttctgac agccaggtac ttaaagattc tgtacagtgg tttaacatcg
     6661 ttggtaacgg ctatgttcaa ctgctgcaaa tgatcgttat gccgttagtc ttcgcctcta
     6721 ttctgagcgc ggttgcccgt ctgcataacg catctcagtt aggcaaaatc agttttctga
     6781 ccatcggtac gcttttgttt accacgctga ttgcggcgct ggtcggtgtg ctggtcacca
     6841 acctgtttgg tttgacggct gaaggtctgg ttcagggtgg tgcagaaact gcacgtctga
     6901 acgccattga aagtaactat gttggtaaag tctctgatct gagcgttccg cagctggtct
     6961 tgtcctttat cccgaaaaac ccgtttgccg atcttaccgg agccaatccg acgtcaatta
     7021 tcagcgtggt aatttttgcc gcattcctcg gcgtagctgc attgaaactg ctgaaggatg
     7081 atgcgccgaa aggtgaacgc gtcttaaccg ctatcgatac cctacaaagc tgggtgatga
     7141 aactggttcg ccttgtcatg cagttgaccc cttacggcgt tctggctcta atgaccaaag
     7201 tggttgcagg ttctaacttg caagacatca tcaaactggg aagtttcgtt gtcgcgtcct
     7261 acctcggtct gctgattatg tttgcagtgc atggcattct gctgggcatt aatggcgtga
     7321 gtccgctgaa gtacttccgt aaggtatggc ctgtgctgac gtttgccttt accagccgtt
     7381 ccagtgctgc gtctatccca ctgaatgtgg aagcacaaac gcgtcgtctg ggcgttcctg
     7441 aatccatcgc cagtttcgcc gactctttcg gtgcaaccat tggtcagaac ggctgcgccg
     7501 gtttgtatcc ggcaatgctg gcggtgatgg ttgcgcctac ggttggcatt aacccgctgg
     7561 acccgatgtg gattgcgacg ctggtcggta ttgttaccgt tagttccgca ggcgttgccg
     7621 gtgtcggtgg tggtgcaact ttcgccgcac tgattgtgct gcctgcgatg ggcctgccag
     7681 taaccctggt ggcgctgtta atctccgttg aaccgcttat cgacatgggc cgtac
//
LOCUS       DADLSA010000166         7630 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.167, whole genome shotgun sequence.
ACCESSION   DADLSA010000166 DADLSA010000000
VERSION     DADLSA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.167"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(268..4170)
                     /gene="hrpA"
                     /locus_tag="JN691_003560"
     CDS             complement(268..4170)
                     /gene="hrpA"
                     /locus_tag="JN691_003560"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAY7244893.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4371..4967
                     /gene="azoR"
                     /locus_tag="JN691_003561"
                     /pseudo
     CDS             4371..4967
                     /gene="azoR"
                     /locus_tag="JN691_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
     gene            complement(5021..6331)
                     /locus_tag="JN691_003562"
     CDS             complement(5021..6331)
                     /locus_tag="JN691_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HAY7244894.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWSHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALPYVVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKL
                     PPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNAVLDVTFEF
                     PRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALV
                     VAAWLLCYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(6321..>7630)
                     /locus_tag="JN691_003563"
     CDS             complement(6321..>7630)
                     /locus_tag="JN691_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HAY7244895.1"
                     /translation="ARPALALWHRLRGLFFINSYVKGRYLTHDRQRVASFNNDPLITR
                     AIAVNILLDLYKTSERIVSDAAAITLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKEL
                     HLLPGFYHDTLGEENRAQAFEKMQSFICRLYANKSQKFDYQHEDRTGPSADRWRLLSG
                     GPVPLSPVDLAYRFMRKAMKLFGAHSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFG
                     RLIDKIYLNSVGWRGIRQRKTHLQILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDAL
                     ANEPAVSDILLRDYSELNVAQGQEMIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLA
                     IVSGLYELFPENEQVKNSLAGLANAIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKP
                     WVMRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT         1860 a         2087 c         1841 g         1842 t
ORIGIN      
        1 cctccttatc gttcccttaa gaatactgcg tgagataata gtgagaagcg gagcactctg
       61 aaaggaggaa gtcgtatttc cttaaccgcc actccgcaag cctgacatat cttttaagga
      121 aatgctgtag ataaacatta ttcatcttgc agctgagaat tgtatttttc tgaattcctg
      181 aatttttgtc cttaccgttt tagaattaat aaaaaacccg gcgctaaatg cttaccgggt
      241 tttagattta ttaggcaaat agcagggtta accgctaatc tgctccatcg cctgcaaaat
      301 acgcttatct gaaatcggat aaggcgtacc aagttgttga gcgaagtaac taacgcgcag
      361 ctcttctatc atccaacgga tctctttcac gtcttcatcc tcacgacgtg caggcggcag
      421 tttgttgatc cattgctgcc acgcctgctg gacgttttcg actttcagca tctgtgcacg
      481 atcgcgatgt ggatcaaccg ccagtttttc cagtcgtttt tcaatcgcct gcaaatatcg
      541 cagcgtgtcg cccagccgtt tgaagccgtt accagtgaca aaaccgcgat ataccaaccc
      601 gcccatctgc gctttaatgt cagaaagccc cagtgccatg gtcatatcca cccgcccttt
      661 cagacgtttg ttgatattga acaccgcagt gaggatttgc tcgacctgct tcgcaatatc
      721 caccaccgtg tcattcagtt cggcgcgcac tttttcatgc agcgcagcaa agccttcttc
      781 cgtccagacc gggccaccat tggcgtcgat cagttgatcc acaccgcagg agatacagtc
      841 gtcgatcagc tccagcactt tgccatacgg gttaaagtac agccccagct tggctttgtt
      901 tggtaacttc tcatgtaaat acttgatcgg cgaggaaata tttagcagca gtagacggcg
      961 aagaccattc cacattgcct gtttttgctc cagcgggtta tcaaacagtt tgatcgccac
     1021 gctgtcgcgt tcatccacca gcgccggcca cgccttcact ttatagttgc cacgcttctg
     1081 ttcgtaactt tccggcagct gaccaaaact ccagatatgt aagccgctct gctcgatacc
     1141 gtcatccgcc accgcggaca gcgtttcctg cactttgcct ttcagcgcat ctttcagatc
     1201 ttgtagcgag cgcccttctt ttagcttctt gtttttgtca tccaccacgc ggaaggtgat
     1261 tttcaggtga tcgggcacct gatcccagtg ccagtcttcg cggtcaacgg taacgccggt
     1321 catccgccgt aactcgcgct caaggctgtc gagcaacggt aactccagcg gtgtgacgcg
     1381 gcctaaaaac gcttcggcgt agtttggtgc aggcacaaaa ttacggcgta ccggtttcgg
     1441 caacgattta atcagagcaa tcaccaattc gcggcgcaga ccggggatct gccattcaaa
     1501 cccgctttcc tcaacctggt taagtaacgg cagcggaata tgtacggtca caccgtccgc
     1561 gtccgcgcct ggctcaaact gatagctcaa acgcagcttg agattgccct gatgccagaa
     1621 gttcgggtaa tccagcttgc tgattttttc cgccccttct ttgatcaaca tgctcttttc
     1681 aaagttgagc aaatcaggcg tttcgcggct gacttttttc caccagctat cgaagtgacg
     1741 agcggagatt acatcgtggc tgatgcgctg gtcgtagaac tcaaacagcg tttcgtcatc
     1801 aaccagaata tcgcggcgac gtgatttgtg ttccagctct tccacttcgg cccgcagttt
     1861 caggttttca cggaagaatg cgtgacgcgt ctgccagtca ccttccacca gagcgtggcg
     1921 aataaagagt tcacgacata acgccgaatc gatctggctg tagttgacct tgcgcgcagc
     1981 aacaatcggc aaaccataaa cagtgacttt ttccgttgcc atcaccgcgc cctgcgcccg
     2041 ttcccagtgc ggttcgctgt aggtgcgttt aatcaaatgc tgagcaacgg gttctaccca
     2101 ttccgggtcg atacgcgcag caatgcgccc ccacaggcgg ctggtttcta ccagttccgc
     2161 caccattacc catttcggcg gctttttgaa taaaccagaa ccggggaaga tggagaaacg
     2221 cgcgttacgt gcaccggtat attcctgttt atcggcatct ttcatgccga tatgggaaag
     2281 caaaccggtc agtaaggcga tgtgaatttc acgatactcc gccggttcgc tgttgaccgg
     2341 aatgccaagt tctttcacca cctggcgcaa ctgggtgtag atatcctgcc attcgcgcac
     2401 gcgcagatag ttgaggtaat cggtacgaca caggcgacgg aaggcgttgg aagaaagcgc
     2461 cttttgctgc tcgccaagat aattccacag atttacaaac gcgaggaagt cggattcttt
     2521 gtcgtggaag cgacgatgtt tttcgtccga tgcctgctgt ttgtccatcg gacgttcgcg
     2581 cggatcctga atggagagcg cggacgtgat aatcatcgcc tcacgcacgc agccatgttt
     2641 ttgcgcttcc agcaccatac gcgccagacg tgggtcgaca ggcaactgcg agagctggcg
     2701 accgagcggc gtcagtttat aggcgctggc ctgttcatcc gtggtgatcg cgcccagttc
     2761 ttcgagcaga cgcacgccat cctggatatt gcgtttatcc ggtgcttcga caaacgggaa
     2821 cgcagcgata tcgcccagcc ccagcgcggt catctgcaaa ataaccgagg ccaggttggt
     2881 acgcagaatc tccggatcgg taaactccgg gcgcgagagg aaatcgtctt cggaatagag
     2941 acgaatacag atcccttcgg acacacgacc acagcggcct ttacgctgat tggcagacgc
     3001 ctgcgaaatg ggttcaatcg gcaaacgctg cactttggtg cgatagctgt agcggctgat
     3061 acgcgctgta ccggggtcga tgacgtattt aatccccggt acggtcagcg aggtttccgc
     3121 gacgttggtc gccagcacaa tgcgccgtcc gctgtgcgac tggaacacgc ggttctgttc
     3181 gctgttcgaa agccgcgcat aaagcggcaa gatttcggta tggcgtaagt tcagcttgtt
     3241 cagcgcatcg gcggtatcgc gaatttcccg ctcgccgctc ataaagatca gaatgtcgcc
     3301 ggggctttcc tggcttagtt cgtctacggc gtcaaaaatc gcctgcaact gatcgcgctc
     3361 ggtgtcatcg gcttcttcaa caatcgggcg atagcgcact tccaccggat aggtccgacc
     3421 ggagacttca ataatcggcg cattattaaa gtggcgcgaa aagcgttccg ggtcgatagt
     3481 cgcggaagtg ataatgattt ttaggtcagg acgccgcggc agcaactctt tcaaatagcc
     3541 gagcagaaaa tcgatattca ggctgcgttc gtgcgcttcg tcaataatga tggtgtcgta
     3601 ctgcatcagc aggcggtctt gctggatctc cgccagcagg atcccgtcgg tcatcagctt
     3661 gaccatcgtg ttatcactta cgtgatcgct gaaacgcact ttgtaaccga tgcaaccgcc
     3721 cggctccgtt ttcagctctt ccgcaatacg gttggccacc gttcgtgccg ccaggcgacg
     3781 cggctgggta tgaccgatca gccctttaat accgcgcccc agctccatac agattttcgg
     3841 tagctgagtc gttttaccgg agccggtttc cccggcgacg atcaccacct ggtgatcacg
     3901 aatcgcttcg agtatgtcct gttttttctg actaaccggt aaattgtcag gataagtgat
     3961 ttccggtcgt gccgcttcac gcagcaggac tttccctgcc gcctggtcaa tctctttcgc
     4021 catctcctgg aaaatggcct gttgtgcatc aggattttta accttcttaa cacaatgcag
     4081 acggcgagaa aaacgcagtc tgtcgcgcag catcagcgaa tccagccgct gttgcaaggc
     4141 agtaaaggtc aatttttgtt gttctgtcat aacgttagtg ggcagtagtc tgcctgatta
     4201 attttctttt aaatgataga tatagagtac cacatcgcgg ctttctatgc gttgttcaat
     4261 aaattcgaac atagacttcg atttattgcg ctatctctgt ggcaggattg tgaataaagt
     4321 gtcagcaagc aacggggcat cgtccattca aacatctata aggaaacacc atgagcaagg
     4381 tattagttct taaatccagc atcctggcag ggtactctca gtctaatcag ttgtccgatt
     4441 attttgttga acaatggcgc gaaaagcact ccgctgatga aatcaccgtt cgcgacctag
     4501 ctgcaaatcc gattccggta ctggatggcg aactggttgg cgctctgcgt ccgagcgatg
     4561 cgccgctgac tccgcgtcag caggaagctc tggcgctttc cgatgagctg attgccgagc
     4621 tgaaagccca cgacgttatc gttattgcgg caccgatgta taacttcaac atctcaactc
     4681 agttgaaaaa ctattttgac ctggttgcac gcgcaggcgt tactttccgc tataccgaga
     4741 acggtccgga aggtctggta acgggtaaaa aagccatcgt tattaccagc cgcggtggga
     4801 tccacaaaga tggaccaacg gacctggtga cgccgtatct gtccacggtc ctcggcttta
     4861 tcggcattac cgatgtgaaa tttgtcttcg ccgaagggat cgcatacggt ccggaaatgg
     4921 cagcgaaagc acagtctgac gcaaaagcag ccatcgacag cattgttccg gacacggtct
     4981 tgatgtggac tgtagatatt cagtccacat ctcaatccac ttacctgttt tcccataatt
     5041 tcaacatcgc tttgtgctct tccttaagca caatatgcga gcgtctggct cgaataaagc
     5101 taatcgcttc atcaactgtt ttacagtgtc cgtaacataa cagccatgcc gccactacca
     5161 gcgcactgcg cgataatccc aatgcgcaat ggaccagaac gctgccttgc tcttcgcgta
     5221 atgtttccag catcgccacg gcctgtcgga gctccccctc ttccggaacc accagatcca
     5281 acatcggtac acagaaatag agtcgatctt ttgtcgctcg tccacgaggg aattcaaagg
     5341 tgacgtccag aaccgcattc tgtgccggaa tatgtcgtgg aaacgccctt aaataaacac
     5401 cagcagtcat tttgctcacc ggctccaggc gacgacaaaa ccaacgcata gatagccaca
     5461 tcccgatgcg ccagggcaat gtcagccagt aaacggcggg cgggagtttc ccctgactat
     5521 ctttccctgt tgttatcgcg ccaagcccac cgtacccacg gccaataatg agtagcgata
     5581 atactggcca acataaccag actgaccacc ataactgaac catcatcatt agctccatca
     5641 acacaatgca cgcgcctacg acatatggca gtgctatttt gattcgacgt tgatcaagtt
     5701 tctgataatt ccaacgacgg tcgacgggca ccatccagtc aatcaacata cctaccgcca
     5761 gccctgtgat gacatcaata aaatgatgct gccatgtcgt cagcgtcgaa atggcgatga
     5821 gtaaaaacca tccgccacag actttacgcc acctcacagc cagatgctga cgaaagtgac
     5881 tccaaagtag ccagcagaga ataatatgca gcgaaggaga ctggttataa ggcagatcaa
     5941 acagttcaag ttgcgaaaat agccatcctg tcaccccact cacttcagga cggataaaac
     6001 taaacttcag cgggtagaga aaaaaaccgc agcaggccat taccgttgcc agaataagcc
     6061 ggtggacaag tcggcgttgt tcgaatgtcg agctacaaac gaacagcgaa aatccatata
     6121 aaagatccag actccagtaa ggaacaatag tccagggaag aaaagggatc gccgtttccc
     6181 aaccgaatac ctgactgggg atatcatggc tgttaaggtc ctgaaccgcg gtgaactgat
     6241 taagagatcc ataggtgaag aagaaaaacg gggccagcaa caataaccag ccagcgcctt
     6301 gtagcaatac gttccgacgt tcagttatca cgacgcaccg ccatcgaaac cgtaaaaatg
     6361 ccccacacat caatccgttg agtgcattta tcaaatccgg cattacgcac gagcgaatcc
     6421 atctcccctt gcgaacgcac gcgcattacc cacggtttac catctttatg actggttaac
     6481 accccggcaa tcagctccag ttgtgggtgc cacggctgcc cggtgtagat gagaatgcca
     6541 cccggttcga tggcattggc tagacctgcg agtgagtttt ttacctgctc attttcggga
     6601 aaaagctcat ataggccaga gacaatcgcc agcgtaggcc gcggagttaa cgcgctgagt
     6661 tcctccgggt taaacgcatc gccctgttca aaacgcaccc gcccagacat tccccgttgg
     6721 gcaatcatct cttgcccctg tgcaacattt aactcactgt aatcacgtaa caaaatatcg
     6781 cttacggcag gctcgtttgc cagcgcatcc agtacatagc gtccatgccc tgcggcaatg
     6841 tcaaccacgc ggacggctaa acctttggcg tggagatcgg caacggcttg tttaatcaat
     6901 atttgcaaat gggttttgcg ctggcgaata ccgcgccatc caacactgtt caggtagatt
     6961 ttgtcgatta aacgcccgaa tgcgttactg ccttgcggtt gattttgata gacataatcc
     7021 agggaactgc ctgaatcaaa gccggtactc attccgagat gcaggcccgc agagtgcgca
     7081 ccgaacaatt tcatcgcctt tcgcataaag cgatacgcta aatcaaccgg cgataatggc
     7141 acgggtccac cagaaaggag ccgccatcga tccgctgatg gtcctgtgcg gtcttcatgc
     7201 tgataatcaa atttttgcga tttgttagca tataagcggc agataaagct ttgcattttt
     7261 tcaaatgcct gcgccctgtt ctcttcaccc aatgtgtcgt gataaaagcc tggcagcaga
     7321 tgcagctctt tcagagggct acgtaatctc tgataaaaat caatctgcgg ttggcgatgc
     7381 accacatagt catcacctga tatcagaagt tgcgtgggga gcgtaatcgc cgccgcatcg
     7441 ctaacaatac gttcagacgt tttatagaga tcgagcaaga tattgacggc aatcgcccgt
     7501 gtgatcagcg gatcattatt gaaactcgcc acccgttgcc gatcgtgggt caaatagcgc
     7561 cctttcacat aggaattaat aaaaaataga ccacgcagac gatgccataa cgccagcgca
     7621 ggacgtgcca
//
LOCUS       DADLSA010000167         7555 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.168, whole genome shotgun sequence.
ACCESSION   DADLSA010000167 DADLSA010000000
VERSION     DADLSA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.168"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(1..1542)
                     /locus_tag="JN691_003564"
                     /pseudo
     CDS             complement(1..1542)
                     /locus_tag="JN691_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1488..1709)
                     /locus_tag="JN691_003565"
     CDS             complement(1488..1709)
                     /locus_tag="JN691_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244896.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLRKAEYITARRPA
                     GQKKKHDAEHSECGTFCDEPEKTRNQDVM"
     gene            complement(1754..3691)
                     /locus_tag="JN691_003566"
     CDS             complement(1754..3691)
                     /locus_tag="JN691_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY7244897.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLAKPVPDMPSQLAARLDEAWAAIK
                     TGLVKGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRQFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEAMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTEFVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            complement(3755..5416)
                     /locus_tag="JN691_003567"
     CDS             complement(3755..5416)
                     /locus_tag="JN691_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY7244898.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYGAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            complement(5413..5976)
                     /locus_tag="JN691_003568"
     CDS             complement(5413..5976)
                     /locus_tag="JN691_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAY7244899.1"
                     /translation="MLTTQKRKFALALMSGKNKTASALAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            complement(6265..6630)
                     /locus_tag="JN691_003569"
     CDS             complement(6265..6630)
                     /locus_tag="JN691_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7244900.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKHHIWLR
                     DAGQCRLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     TTLPEVSGGSGRADDIIGL"
     gene            6672..6872
                     /locus_tag="JN691_003570"
     CDS             6672..6872
                     /locus_tag="JN691_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HAY7244901.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            complement(7004..7330)
                     /locus_tag="JN691_003571"
     CDS             complement(7004..7330)
                     /locus_tag="JN691_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HAY7244902.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVRFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
BASE COUNT         1659 a         2375 c         1826 g         1695 t
ORIGIN      
        1 tcaagcatcg gacgcatatc atcgcccgtc acgcacttct gtaataccac agacagggac
       61 gccagttttt cttcaaacgt ttgtgcctgc gcggctattt tcccctcaaa tgtgcgctgt
      121 aaatccgcca gcactgcgga aaattcttca cccagcgcac ggataatgga cagttcacgt
      181 tcattcattt tttcagaatc cccctgaaca tcgccttcac cgcatcatgc tctgtttcag
      241 tgattgcctt attaccgtca gatgcgctgt caggcggttg cgctaaggcc gttttcccgg
      301 tcgacgcgaa cggatcttca cgggcatcac gacgggacag cgcctccaga ctgtagttct
      361 gctgctgaag atacagtgca tcacctcccg caaggggcgg caggttctca cgtttacggg
      421 cctcattggg cgtgagaagc gtatttttca ccgactcacc cagcgttttc atgcgccgtt
      481 cgctgtccat tctcagcagc gtggtgacgt caaactccgt actctcgttt tcccccgttt
      541 ccagcgcctc atccagtaac agttcaatgg actcaatcag cgtctgcagg cactgggaat
      601 aatactgctg ctccagcgcc tccacgttgt cactggaagg tggctggcca acgccaatct
      661 tgtaggccgg gacacggaac accgaacaga caatttcagc ggtcatcttc agttgttcca
      721 ccgtctgcgc atccaccggt gaaaacgtcg tggggttgta ttttgccccg ttgctcaaaa
      781 tggccgtttt ccccgcattt tcgccggtat acccgctgtc ccagttgctc ttcagttttt
      841 tcgcattttc ttccgtaata ctgccgggga tctcaatcac cccggacggc ctgccgccat
      901 ttctgaaaaa agacgtcgaa ttttcctgaa tatgatgccc ctgcgtggcc gccagcccgg
      961 cggcatacac cggcggcagc cccacaagcg gatgaaaaaa acagttaaac cggtcgcttc
     1021 ccgggcaggc accgtcaccg cctccgtgat cccgcagttc cggtccggcg tgatgcgata
     1081 gaacacctcg ccgtcatccg ccaccagagg ttcaacccgg ctccagtcca gaatacgcag
     1141 ttctttgatc tgcccccggg agttacggat tttcagcacc accgtattgc cgtgacgcag
     1201 tttggcgttc agccacagtt caaaaaactg gatacgattc tgctgtgcat tgggacgacg
     1261 acagagacgg gcaatatccc cctgccgttt ttcacggcgg atcccctgtg tatccgtctg
     1321 catcaggcgc agccgcattt tggcgatatc ctgggatatc agcgaaatgc aagaaaacac
     1381 cgcatgaaag gagaggacac tttccggatc ggctttcaca ccctgctgcc aggcgccagc
     1441 aaagggctca gccaccgcct gaaacaggct ggtccagccc acctctttta catcacgtcc
     1501 tgatttctgg ttttttcggg ttcgtcgcaa aaagttccac attcgctatg ctccgcatca
     1561 tgtttctttt tctgacccgc cggacgtcgc gctgtgatgt actccgcctt cctcaggcga
     1621 accagcacct ccgcacacgg ctgtgccaca tcacggatat ccccggcccg ggcatcatgc
     1681 gtgccctgca gatatcggat ctttgccata acctgttacg ggaggcgcac gcctcccgtc
     1741 ctccttatca gactcagccg ccggacgcac tgccgtagtt cactccggtg atcaccgcca
     1801 ccgccgcggt acggcgacga cgccagttga tccagcgctc cgcacggatg gccacactgc
     1861 ctgtctggaa catggaaacc agctccaccg gcgacggcgt ggtactgtcg ccggtcggct
     1921 cagactgcat ttccagtgat gcctcgcggg acatatccac tgccacgccg ccgtcatccg
     1981 ccagataaat atccggggca ttcaccagca ccagctggtc acccacgtac tgggagacaa
     2041 tcaccggcag cccctggaag gagccaccca gcagggtcat gtccgggtat tctttctgac
     2101 ccagcgcatt tttacgcatg gacagcgcca gggcattggt gctggacatc agccagacag
     2161 caccggtggg ctgcaggttt gctgccacaa actgtccaaa cgccgcctct gcatccgcat
     2221 ccgggttacc gcttgatgcc gtgcccttca catcatgggt gatggacgcc ggggagacat
     2281 ctgccaccgc cgcttttttc gggtccacaa attctgtatc cagacgcgcc accaccgctt
     2341 ccgccagcgc attacggacc agtgcatcag cagccggact ggaaaaacgg atcaattctt
     2401 ccgtcagtac cgcaatggcc gacaccttcg catgactgaa ggtgatggat tcaaaatcaa
     2461 acttcgtcag gggttttgcc ttaccctcac ccacccagcc ggcagcaccg cccgacacct
     2521 gggcgtgcac acggatattg aacggcacct gacgaagtgc agggatcccg ccctgaccaa
     2581 atcgcccgat aatggtctga ggacgcaggt aatcaataaa gtcctgtgcg tattcctgat
     2641 attcagacag gctgcctgcc cactgcggat ccgtggtggt ccccgcgccc actgccgatt
     2701 tcaggacatg atgcagacga ctgtcatccg gatactgacg acgggccact tccagggctt
     2761 cagagcggac acctttagcc gcagccagcg atttggcaaa acgtgcgaaa ccaatcccct
     2821 tatccagttt ctgctccaca cggatcaccg gcgcagaagc caccgcggcc acattcccgt
     2881 taccggcctg tttcaccggc tgcgccgtgg cggccttacc ggcttccagt tcacgcaggc
     2941 gcttcaggtg cgcatccacc tgacgtattt ccgctgcggt gttgtcgtaa tgctcttcct
     3001 cctccacatc cagcgtgcgc ccttcctctg cggctttggt catggcctcc tcaagggagg
     3061 ctgccagtgc tgcacgcttg ttttcaaaac ttttaatctg ttcgccaata ttcattatgg
     3121 tcttttcctt atgaaaaacg gttgttgact gtgccgcagc gccggcagaa gatgcgattt
     3181 tcaccaccgg tttccggttg ccggacgcgg cagaaaactg gcggtcgtaa gatttaatgg
     3241 tccggatggt gcattccgca ttcgcgggca cggtgacggc agacacctcc atcagctccc
     3301 agcgcagaaa atgcagtccg cctccgtcca gaaaggtgta ttcatgagga cggaagccca
     3361 cggacagccc cttgaccagc ccggtcttaa tggccgccca ggcctcatcc agccgggcag
     3421 ccagttgcga tggcatatcc ggcacgggct tcgccagtgt tgccgtgatt tccagccctt
     3481 cgctgacccg gcgcaccgta cactgcccca ccgggcggga atggtcatgc tgccagagaa
     3541 acgggatcgc actgccaaac tccgcgccct ccggctccag gatgtcacca tcccgatccg
     3601 gagaaggcgt tgacgcaatc ccggtgatca cccgctcatc ctcactgaag gatttcaccg
     3661 tcagcaggga gcaggcccgt ttaagagtca catcagcctc ctgaaaataa aaaaaccgcc
     3721 gcagcggtcc atgatggtta cagggtgaac agggttatat gaaaaaaacc gcatattctt
     3781 tctttttcgg ctccggattc agggacatca gggataccgc attgaagagc gccatcagcg
     3841 ggtcaatttt cccccgcccg ctggcctgtt tggtaataag gatagcgtta cctttaggct
     3901 ccacccgggc attgccaacg caccaggcca tcagtggctg accaccatgc accagtactc
     3961 cctcagccag tttgcgctcg gtggttttta tggccccgcc cagcttccag ccctggctta
     4021 tccccacaac aattccgtcg gggatcccgg cttccgccag tgaatccaga atctgcccca
     4081 cacctgacgg gtcaataccg atatgctcca gtaactcagc ctcatgaata cgacgcacat
     4141 actccgccac ttccgccgtg tcatccccga cccgacggac aatcgtcatg tctccacagg
     4201 ccacaaaatc ctgaaaacgg gatgcctcac tcttccgtct gaccaccgcg gtttcatgcg
     4261 cccaggcatg gccccagccc agccattcgc gggtttccct gtcacggcca atcacgtaca
     4321 ttcccagcag atcatccagg cccccgccgt caatccccac cgtcaccaca tcagcgcgct
     4381 gcaggatatc gtccaggctg acgcgcctgc cctgctgctc ccagaaatcc gcacccgccc
     4441 agcggtcaga acgcagggca agaccaattt ccacattggc atgttttgac atgaagccac
     4501 gaaatgcttc ctcaccagcc tcccgggctt tacggtactc ccggtacaga aaagcctcat
     4561 ccaccgaata accgagattc ggattgacca tggcgaggtt ttccatcagc aggtgagccc
     4621 cgctttccac catttcagga gggtgttcaa atatcaccgg cagaaagtgc ggatcatgaa
     4681 ttttgccgtc acggacatcc cgggcgtact gcagtttctg tctgaacacc ccggctggcg
     4741 gctcattcga ctgggtggtc gtatacacca caaacccttc cgggcgggag gcaaggccgc
     4801 ctatggcttc acgtaacatg tcctccgctt tgtactgctt gccaaataac cacagttcat
     4861 caatcagcgt ccccacggac ttgataccgg ataccgtatt cggatcggca gccaccacct
     4921 taagggtggt gtccgtcacc ctgtgggtga tggtccggat atgtgtctgc acctgacaga
     4981 ggtcatccag atcatcgtcc cgtcgtacca tatccctagc agggttgaag gcgttagccg
     5041 ccacctccac ggtcggggcc agaatggtat agcccgccgc ctgccgccag ttcagtaaca
     5101 gcgccgtcat catgatcccg gcagccagcg tggacttgct gtttttcttg gggataagga
     5161 taaaaacttc cttgatatgg cgtacaccgg tctgcgcacc gtaggagcca aacagggccg
     5221 ccaccaggtc aaacacccac tgtgcgcagg actccccgaa cgtcgggctg cccggtgcat
     5281 ccacaatccg cagttgttta aaaatcgcca gggcatgtgc ggcctgctcc ggataaatcg
     5341 gagccggaat aatcgacagc ccctttttca ggcgctctgc ccagtccggg caggccgtgc
     5401 tccacacagg tatcatccgt tgccctcatt atcattattc accaccaggc ggggtggtgg
     5461 tggcaccgca aaacggttag ccgctttttt cgccgcgtca ccttttgccg attttttacc
     5521 ggcatcccct tttttgtggt gcgtgaactg cgccagctta taagccgcat ccagcgccag
     5581 cctggggtcg gtattaatgt tctccaccag aagacgcccc atcgctttca ccggatcggg
     5641 aagaccgtcc tccatatact caataccagg agatatcacc acgggcggtg gcatctccgg
     5701 atttgtttcg tccggctgtg gtattgcagc cgcctcacgg cgacggggtt tatcctccgg
     5761 ctctgatttt ttctgccgat aaacaggaac ctcatccacc tccaccgtct cgcactgttt
     5821 acgggctata aacgcaagca cctccggatc ttttgccagc tgcgagcctt taaccctggc
     5881 tgtcttcgcc gaataaccgg cggcaagggc tgacgctgtt ttgtttttcc cggacatgag
     5941 cgccagcgca aattttcgtt tttgcgttgt cagcacagcc tcctcccggg tccagaacgc
     6001 actcagccgg gtatggttca gcccattttt cccggcgtct catgccgcaa atgttaactg
     6061 ctgcctggtt aacatttgct gaaaaatcct gttaacattt tttccacgca acaaactgaa
     6121 taataaagat aaaaaccgca aaaatgcccg gacagccagt taacatgtta actgccctga
     6181 aacgggaatt ttttctctgc gtgagagggg gcgcggtgtc cggagcgatc gttttttacg
     6241 ccgaatgata cccccccggt cgggttacag tccgatgatg tcgtccgctc tgccactacc
     6301 tccggacacc tccggcagcg tcgtgtccgg cataccaccc gccgcttcac gagcagactt
     6361 ttgtcgatgg cattcggtac agagcgtcca gagattcgtc tcctcattac caccaccgaa
     6421 ctgaagtgca attcggtgat cgagttcact gtcacagagg tcaaccacac gaccacagag
     6481 acggcactgc ccggcgtccc tgagccagat atgatgcttg agggaaacac gtgcactgcc
     6541 actgacacga cgctgttcac ccttcagaat attcacccgt cgggtattca gtgttttgat
     6601 tctgctctgg agtgtacgaa gctcagccat gtaaaatccc cgtcatatgg caatcagtaa
     6661 aggaaataaa tatgtcatcg aaaaaccgta cccgcagaac cacaacccgc aatatccgtt
     6721 tccccaatca catgattgaa cagatcaaca tcgcccttga gcataaaggg tccggtaact
     6781 tttcagcgtg ggttattgaa gcctgcagga gaaggctggc aacagatgca acgcatctgc
     6841 gcccggccag catgacaaat aacgagaaat gaacgttcgg ttacaggagc aggtacccac
     6901 tgtcctccaa caatatttca tcttcatatc cgacggaaca agacttaccc tgccgggatg
     6961 tacagaataa caacagagtg ataattaatt tctgatgaaa taatcagggt gcagaaggac
     7021 taaagataaa cgttttcttc acgcctttac acggcctgtc cttctcaaat cgccattttg
     7081 ccatcgcctt tacaacctgc tcatcaaaca gatggtgcgg ctctgaacga ataaactcaa
     7141 ttcgggtgac agtaccatca gcaccaatat caaacctcac atcaacccgt ccctttatat
     7201 aatttgccgc tgcataggcc ggatattgtg gtaatgcctt aaccaactgt cggggcatat
     7261 ctgttttatg ttgcgtacag cccataacca gagaagacaa caaaataatt aacggaagat
     7321 ttcttttcat tttcattccc cgcacagata agaataagtc ttattctaac aatgccgccc
     7381 tgtcggtcat caatcctctg cttaatggca acgacaatta tccgacttaa atcacaaatc
     7441 agacacatga cataacaggg cttgcgaggt aacacatcgt cctgtttctt ccaccatcgc
     7501 accggactgg cgactatgag gggacaacgc cgcgctccgt taacgcggta aaccc
//
LOCUS       DADLSA010000168         7528 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.169, whole genome shotgun sequence.
ACCESSION   DADLSA010000168 DADLSA010000000
VERSION     DADLSA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.169"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            556..1326
                     /locus_tag="JN691_003572"
     CDS             556..1326
                     /locus_tag="JN691_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAY7244903.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1317..1790
                     /locus_tag="JN691_003573"
     CDS             1317..1790
                     /locus_tag="JN691_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAY7244904.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1897..2436
                     /gene="dnaT"
                     /locus_tag="JN691_003574"
     CDS             1897..2436
                     /gene="dnaT"
                     /locus_tag="JN691_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAY7244905.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2439..3176
                     /gene="dnaC"
                     /locus_tag="JN691_003575"
     CDS             2439..3176
                     /gene="dnaC"
                     /locus_tag="JN691_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAY7244906.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3225..3719
                     /locus_tag="JN691_003576"
     CDS             3225..3719
                     /locus_tag="JN691_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAY7244907.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3973..6264
                     /gene="opgB"
                     /locus_tag="JN691_003577"
     CDS             3973..6264
                     /gene="opgB"
                     /locus_tag="JN691_003577"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAY7244908.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6402..7424)
                     /locus_tag="JN691_003578"
     CDS             complement(6402..7424)
                     /locus_tag="JN691_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAY7244909.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
BASE COUNT         1914 a         1951 c         1833 g         1830 t
ORIGIN      
        1 tttccagcaa attaatagat taagcgaata aattatcctt agcattcact ttcatatcag
       61 catactcctg ctgcacaaca actccactgc atgtgtcaga aggttagcat caaatgataa
      121 cctggtgaaa tagcctggag acatttaaaa tatctacatt aagttgttag aaatacttaa
      181 tgaaaaacag aaatctcatt catttaattt ataattaaat gatatctctt cccgcagaat
      241 gcgggaatca cacaatcaat atttattcaa taaaatctga tgattttaac ttgttaacac
      301 taaaaaacgc gcccagctat ctattatcct gacccgtaaa ggctaaacaa taaattcatt
      361 atagtgataa tattcatgtg atagtttgtt tgcggcgaga gataattcgc tttttatcac
      421 cgagcttgtc ccaataacca ggattaatca gcgcaatttt gatcttaacc taccgcaaaa
      481 caaccgtgct tcatgataca aatggattct cattatctga acaatacgca gcacgtctat
      541 gacaaagggc gagttatgca aactgagcaa cagcgagccg taacgcggct ttgtatccag
      601 tgtggattat ttcttttgca acatggtgcg gaaagcgcgt tggttgatga gctttcctca
      661 cgactgggtc gggcgctggg aatggacagc gtcgaaagtt ctatctcttc gaacgccata
      721 gtgctgacaa ctattaaaga tgggcaatgc ctgacatcga cacgtaaaaa tcacgatcgc
      781 ggcattaata tgcatgtggt gactgaagtc cagcacattg tgattcttgc ggagcatcat
      841 ctgctggatt acaaaggcgt agagaaacga tttagccaaa ttcaaccatt acgttaccca
      901 agatggctgg tagccttaat ggttggcctt tcttgcgcct gtttctgtaa actcaataac
      961 ggtggctggg atggtgccgt catcaccttc tttgccagta cgaccgcgat gtatatccgc
     1021 cagctgctgg cacaacgtca tcttcatcca cagatcaact tttgccttac cgctttcgcc
     1081 gccaccacca tttccggatt gcttttgcaa ctccccactt tcagcaatac ccccaccatt
     1141 gcgatggccg ccagcgttct gctgctagtg ccgggctttc cgttgattaa tgccgtcgcc
     1201 gatatgttta aaggccacat caataccgga ctggcacgct gggcgatcgc cagtctgctg
     1261 acactggcta cctgcgtcgg cgtagtgatg gcactgacga tttgggggct acgcggatgg
     1321 gtgtgatcga atttctgtta gcgttggcgc aggatatgat cctcgccgcc attcctgcgg
     1381 tcggctttgc gatggtgttc aacgttcccg tgcgggcgtt acgctggtgt gcgctgcttg
     1441 gctcgatagg tcatggttcc cgaatgatct tgatgaccag cgggttgaat attgagtggt
     1501 caacctttat ggcttctatg ctggtcggta ccattggtat tcaatggtcg cgctggtatc
     1561 tggcgcatcc gaaagtgttt accgtggcgg ccgttatccc tatgttcccg ggcatatcgg
     1621 cttataccgc aatgatttcg gcggtaaaaa tcagccagtt aggttacagc gaaccgttga
     1681 tgattaccct gttaaccaac tttcttacag cttcatcgat tgttggtgcg ttatccatcg
     1741 gtctttccat tcctggatta tggttgtacc gcaagcgccc tcgcgtataa aattgcctcg
     1801 cttaagcaca cggatgagag acagcctcct ctcctccgtg tgttactata aaagttatct
     1861 cccttctcgt tcatcgttcc atatttgaga aacagtatgt cttccagagt tttgaccccg
     1921 gacgtcgttg gtattgacgc cctggtacac gatcaccaaa ccgttctggc aaaagctgaa
     1981 ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt tttatgccgt cacgcctgca
     2041 cgcctggctg aactgctggc gctggaagaa aagctagcgc gtccgggaag cgatgtcgct
     2101 ctggacgatc aactctatca ggaaccgcaa gccgctcccg ttgctgtacc catggggaaa
     2161 ttcgccatgt atccggactg gcaacccgat gccgatttta tccgcctggc ggcgctatgg
     2221 ggcgtggcgc taagagagcc ggtgaccacc gaagaactgg cctcattcat tgcctactgg
     2281 caggcggaag gtaaagtctt tcaccatgtg cagtggcaac aaaaactggc gcgcagcctg
     2341 caaatcggtc gtgccagcaa cggcggactg ccgaaacgag atgtgaatac ggtcagtgaa
     2401 cctgacagcc aaattccacc aggattcaga gggtaacgat gaaaaacgtt ggcgacctga
     2461 tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc tgcattcaaa acgggtgaag
     2521 agctactggc ctggcagaaa gaacaagggg cgatccgctc cgccgctctc gaacgtgaaa
     2581 atcgggcgat gaaaatgcag cgcaccttta accgctccgg tattcgtcca ctgcatcaga
     2641 actgctcctt tgagaactat cgcgttgagt gtgaagggca gatgaatgcg ttaagcaaag
     2701 cgcgccagta tgtcgaagag tttgacggca acatcgccag cttcatcttt tctggtaagc
     2761 caggaaccgg caaaaaccat ctggcggcgg caatctgcaa cgagctgctg ctacgcggta
     2821 aatccgtatt gatcatcacc gtggccgata ttatgtcggc gatgaaagat accttcagga
     2881 atagcggtac cagcgaagaa caactgctta acgatctgag caacgttgat ctgctggtga
     2941 tcgatgagat cggcgtgcag accgaatcga aatacgaaaa agtgatcatc aaccagatcg
     3001 tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct gaccaacagt aatatggaag
     3061 agatgaccaa gttgctgggc gaacgcgtga tggaccgtat gcgcctgggt aacagtttat
     3121 gggtgatctt caactgggat agctaccgta gccgggtaac gggtaaagag tattaatccc
     3181 cgtttctggc ggtatactgc gacacaattc caccttgagt aatgatgatg aaaactatta
     3241 aacatcttct gtgctgtgcc attgccgcca gcgcattaat ttccaccggg gtgcatgctg
     3301 cgtcctggaa agatgcgctc tccagcgccg ccagcgaact tggcaaccaa aacagcacga
     3361 cacaggaagg cggttggtcg ctcgcgtcat taactaactt gcttagcagc ggaaaccagg
     3421 ccttaagcgc agataacatg aacaatgccg caggcattct gcaatactgc gcgaagcaaa
     3481 agctggcttc ggtaaccgat gccgaaaaca tcaagaacca ggtgctggaa aagctgggcc
     3541 tgaacagtga agagcaaaaa gaagacacca actatctgga cggtattcag ggtttgctga
     3601 aaaccaaaga tggtcagcaa ctcaatctgg ataacatcgg aacgactccg ctggcagaaa
     3661 aggtgaaaac caaagcctgc gatctggtgt taaagcaggg gctgaacttc atttcctgaa
     3721 ctgcgaaacg ccgcgtttta gctgttaccc ggctgacgcg gctctctcct gcccttttcc
     3781 ctgctcctgg acggttttcc ccctgttaca cgggaagcga ccgcgatcaa aaaagtctgt
     3841 ttataaacta attcctaaca acatcgcatt tttttgacac tagaattgca gcaatatggc
     3901 gacgccatta cattgtgctt ctcaaaaagc ttaaacgttg gctaaacggc ccggtttagt
     3961 gaggatcgcc cgttgtcaga actactctct ttcgcccttt ttctcgcctc tgtgctgatt
     4021 tacgcatgga aagcgggacg taacacctgg tggtttgcag ccacgttaac ggtgctgggg
     4081 ctatttgtcg ttttaaatat caccctgttt gccagcgact attttactgg cgatggtatt
     4141 aacgacgcgg ttctctatac cttaaccaac agcctgaccg gtgctggcgt cagcaaatac
     4201 atcctgccgg gtatcggcat tgtgctgggg ctgacggcgg tgttcggtgc gctgggctgg
     4261 atcctgcgcc gtcgtcgtca tcatccgcac cattttggtt acagcctgct ggcgctctta
     4321 ctggcgctgg gttcggtgga cgccagcccg gcatttcgtc agataacgga actggtgaaa
     4381 tcccagtcac gcgatggcga cccggacttt gcggcttatt ataaagagcc gtcgaaaact
     4441 atccctgacc cgaaactcaa cctggtttat atctacggcg aaagtctcga gcggacctat
     4501 tttgataacg aagctttccc ggatctcacg cctgaactgg gcgcgttgaa aaatgaaggc
     4561 ctggatttca gccacacgca gcagctgcca ggaacggatt acacgattgc gggcatggtg
     4621 gcttctcagt gcggcatacc gctgttcgcc ccctttgaag gcaacgcctc cgcctctgtc
     4681 tccagcttct tcccgcagaa catctgtctg ggcgatatcc tgaaaaactc gggttatcag
     4741 aactatttcg tgcagggcgc aaatctgcgt tttgccggta aagatgtgtt cctgaaatcg
     4801 cacggcttcg accacttata cggctcagaa gagctgaaaa gcgtggtagc cgacccgcac
     4861 tatcgcaacg actggggatt ctacgacgat accgttctcg atgaagcgtg gaaaaagttt
     4921 gaagagcttt cccgctcagg tcagcgattc tcactgttta ccctgacagt cgatacccat
     4981 cacccggatg gttttatctc gcgcacctgt aaccgcaaaa aatatgattt tgacggtaag
     5041 ccgaatcagt cattcagcgc ggtaagttgc agccaggaga atatcgcgac gtttatcaac
     5101 aaaatcaaag cgtcaccgtg gtttaaagat actgtcatcg tcgtctcttc tgaccattta
     5161 gcgatgaaca acacggcgtg gaaatacctc aataagcagg atcgcaataa cctgtttttt
     5221 gtcattcgtg gcgacaagcc gcagcaaaag acgctggcag tgaagcgtaa cacgatggat
     5281 aacggcgcga cggtgctgga cattctcggt ggcgataact atctcggact tggtcgtagc
     5341 agtttatccg ggcagtcgat gtcggaaatc ttcctcaata tcaaagagaa aacattggcg
     5401 tggaagccag atatcatccg cctgtggaaa ttccctaaag agatgaaaga gttcaccatc
     5461 gaccagcaga aaaacatgat tgccttctcg ggtagccatt tccgtttgcc gctgctgttg
     5521 cgggtttcag acaaacgcgt ggaaccgctg ccggaaagcg aatactcagc accgctgcgt
     5581 ttccagctgg ccgatttcgc tccacgcgac aatttcgtct gggttgacag ttgctacaag
     5641 atggcacaac tctgggctcc ggaactggca ctttccaccg actggtgtgt ctcgcagggg
     5701 cagcttggcg gccagcaaat tgttcagcat gttgacaaaa caacatggaa gagcaagacg
     5761 gcatttaaag atacggtcat cgacatggcg cgttacaaag gcaatgtcga tacgctgaag
     5821 attgttgata acgatattcg ctacaaagcc gacagtttca tctttaacgt cgccggtgcg
     5881 ccagaagagg tgaaacagtt tagcgggatt tcccgtccgg agtcgtgggg ccgctggtcc
     5941 aacgcgcagc tgggcgatga agtaaaaatc gagtacaagc atccgctgcc gaagaaattt
     6001 gacctggtga ttaccgccaa agcatacggc aataacgcca gccgtcctat tccggtacgc
     6061 gtaggcaatg aagaacaaac tcttgtgctg ggcaatgaag tgaccaccac cacactgcat
     6121 ttcgataacc caaccgatgc cgacacactg gtaattgtgc cgccggaacc tgtctcaacc
     6181 aacgaaggga atatcctcgg acactcgccg cgtaagctcg ggatcggcat ggtggaaatt
     6241 aaagtggtag aacgtgaagg gtaattcctg actgatgtgt aggtcgcata gggtgcgacc
     6301 tacacgcctt ctccggctta caacacatta ttaagaaaca aaaaaaacag cgcccaacac
     6361 ttccccggac gctgcatggc aactttaata atctcgcctt atcagaaagt aattacaccc
     6421 ttaatcaact cacgattgtt aatcacatcg cgctcgtagg tttctgccag cgtggcgaac
     6481 ggatagcgat gggttaacat catgtcggca gtgattttcc cttccgccat cagtcgaccg
     6541 actttagcga aatcttccgg cgtggcgttg cggctgccca tcatcgtcgt ttctttttta
     6601 tggaattccg gatcggagaa ctgcaactca cctttaaaca gaccgacaaa taccaccgtg
     6661 ccgccgtgac gaatcagatt cacggtgtta ttcatcgcat gttgattacc tgtcgcgtcg
     6721 atcactttct gcgccagcga accaccaaac tgcgcccgca gctgcgcgtc aaaatcttcg
     6781 gctgacgggt ccagcacggg caactccaga cgcgttgcca catgttcacg gcgcgccgga
     6841 ctggtatccg ccaccaccac ctgtgcgcca tcggctttag cgattgccgc cgcgcccaga
     6901 ccgattggcc ccgccccgac caccagcacc tgctcgccgg gagcaatggc tgcgcgacgc
     6961 accgcatgag cgctaatagc gaaaggttca atcaatgcgg ccgcctgcgg gtcaataccg
     7021 tctgcgggca aaatgttcgc caccggcacg ctcaaatact cactaaaacc gccatcctga
     7081 tgcacgccaa tgactgaaat tttttcacag cagttggtac gcccgctttt acacgccggg
     7141 cattgctgac aggcaacata agggatcaca gcaacttgct gaccattttt aagatcagca
     7201 atatttttac ccagcccaac aatctcccca catatttcat ggcctaaaac ccgtggataa
     7261 ctaaaaaatg gttgatttcc accccaggca tgaatatcgg taccgcaaat cccgacagac
     7321 ttaattttta ttaatgcttc attgtcaccc ggaataggta tctcgcgttg tttccagact
     7381 aattctttcg gctgctggca aattaaaaca ttcatcgtag acatgattcg gctctccatt
     7441 ttttgttgcc ttagatatca atgaaaatga aaaatggcag cggaacggaa aatctgtttt
     7501 gtgaaaacac acgcataaaa atgtttta
//
LOCUS       DADLSA010000169         7506 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.170, whole genome shotgun sequence.
ACCESSION   DADLSA010000169 DADLSA010000000
VERSION     DADLSA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.170"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            1..1183
                     /gene="pgaB"
                     /locus_tag="JN691_003579"
                     /pseudo
     CDS             1..1183
                     /gene="pgaB"
                     /locus_tag="JN691_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309296.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
     gene            1176..2516
                     /gene="pgaC"
                     /locus_tag="JN691_003580"
     CDS             1176..2516
                     /gene="pgaC"
                     /locus_tag="JN691_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HAY7244910.1"
                     /translation="MINRIVSYRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPF
                     FMSIMWIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAA
                     LAQRYENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQKQGKAIALKTGAAAAK
                     SEYLVCIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSI
                     IGLIKRTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIF
                     YEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLT
                     TIWAFTCLAGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRY
                     EHNLTSSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            2518..2931
                     /gene="pgaD"
                     /locus_tag="JN691_003581"
     CDS             2518..2931
                     /gene="pgaD"
                     /locus_tag="JN691_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HAY7244911.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(2981..3904)
                     /gene="phoH"
                     /locus_tag="JN691_003582"
     CDS             complement(2981..3904)
                     /gene="phoH"
                     /locus_tag="JN691_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HAY7244912.1"
                     /translation="MRSRFSAFYPKANYGLQGSQPSDVRVHNRAANGACDEYKQLKVL
                     SMGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEAIGMARDSRDTSPI
                     LARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHKDVDRIIVTRPVLQ
                     ADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIGKVETAPFAYMRGR
                     TFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLPRGVCSGLSDALER
                     FEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(4377..5642)
                     /gene="efeB"
                     /locus_tag="JN691_003583"
     CDS             complement(4377..5642)
                     /gene="efeB"
                     /locus_tag="JN691_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HAY7244913.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(5648..6775)
                     /locus_tag="JN691_003584"
     CDS             complement(5648..6775)
                     /locus_tag="JN691_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HAY7244914.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(6833..>7506)
                     /locus_tag="JN691_003585"
     CDS             complement(6833..>7506)
                     /locus_tag="JN691_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HAY7244915.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1882 a         1921 c         1773 g         1930 t
ORIGIN      
        1 aaatgcgtca caattggatt ccattccgcg agtattaatc gccaataatc cctcattaaa
       61 agagtttgcc cagcaaatta ttaccgtaca ggaaaaatca ccacaacgga taatgcatat
      121 cgatcttgat tacgtttatg acgaaaaccg ccagcaaatg gatcgcaata ttgatgtgct
      181 aattcagcgg gtgaaagata tgcaaatatc aaccgtgtat ttgcaggcat ttgctgatcc
      241 cgatggtgat gggctggtca aagaggtctg gtttccaaat cgtttgctac caatgaaagc
      301 agatattttt agtcgggttg cctggcaatt acgtacccgc tcaggtgtaa acatctatgc
      361 gtggatgccg gtattaagct gggatttaga tcccacatta acgcgagtaa aatacttacc
      421 aacaggggag aaaaaagcac aaattcatcc tgaacaatat caccgtctct ctcctttcga
      481 tgacagagtc agagcacaag ttggcatgtt atatgaagat cttgccggac atgctgcttt
      541 tgatggcata ttgttccacg atgatgcttt gctttcagat tatgaagatg ccagtgcacc
      601 ggctatcacg gcttatcagc aagcaggctt tagcgggagt ctgagcgaaa ttcgataaaa
      661 cccggagcaa tttaaacagt gggcccgctt taaaagtcgt gcgttaactg acttcacttt
      721 agaacttagt gcgcgcgtaa aagccattcg cggtccacat attaaaactg cacgaaatat
      781 ttttgcactt ccggtaatac aacctgaaag tgaagcctgg tttgcacaga attatgctga
      841 tttcctaaaa agctatgact ggaccgctat tatggctatg ccttatatgg aaggtgtcgc
      901 agaaaaatcg gctgaccaat ggttaataca attgaccaat caaattaaaa acatccctca
      961 ggctaaagac aaatctattt tagaattaca ggcacaaaac tggcagaaaa atggtcagca
     1021 tcaggctatt tcttcgcaac aactcgctca ctggatgagc ctattacaac tgaatggagt
     1081 gaaaaactat ggttattatc ccgacaattt tctgcataac caacctgaaa tagaccttat
     1141 tcgtcctgag ttttcaacag cctggtatcc gaaaaatgat taatcgcatc gtatcgtatc
     1201 gtatcgtatc gttttttata ttatgtctgg tgttatgcat acccctatgc gtagcgtact
     1261 ttcactctgg tgaactgatg atgaggttcg ttttcttctg gccgtttttt atgtccatta
     1321 tgtggattgt tggcggcgtc tatttctggg tctatcgtga acgccactgg ccgtggggag
     1381 aaaacgcacc agctccccag ttgaaagata atccgtctat ctccattatc attccctgtt
     1441 ttaatgagga gaaaaacgtt gaggaaacca tacacgccgc tttagcacag cgttatgaga
     1501 acattgaagt tattgccgta aatgacggtt caacagataa aacccgtgcc atcctggatc
     1561 gcatggctgc acaaattccc catttgcggg tcattcatct ggcgcaaaaa caggggaaag
     1621 ccattgcgct taaaaccgga gctgccgcgg cgaaaagtga atatctggtg tgcattgatg
     1681 gcgatgcgtt attagaccgc gatgcggcgg catatattgt ggaaccgatg ttgtacaacc
     1741 cgcgtgtggg tgccgtaacc ggtaatcctc gtattcgaac acgttctacc ctggtgggta
     1801 aaattcaggt tggcgagtat tcctcaatta ttggtttgat caagcgaacc cagcgtatct
     1861 atggaaacgt atttaccgtt tccggtgtta ttgccgcatt tcgtcgcagc gccctggcag
     1921 aagtgggtta ctggagtgac gatatgatca ccgaagatat tgatattagc tggaagctgc
     1981 agttgaatca gtggacgatt ttttacgagc cacgggcgct gtgctggata ttaatgcctg
     2041 aaacgttaaa agggctgtgg aaacagcgcc tgcgctgggc tcagggcggt gcagaagtat
     2101 tcctcaaaaa tatgacaagg ttgtggcgca aagaaaactt tcgaatgtgg ccgctgtttt
     2161 ttgaatactg cctgacgaca atatgggcct tcacctgcct ggccggtttc attatttacg
     2221 cagtccaact tgccggtgta ccgttaaata ttgaattgac acatatcgct gcgacacata
     2281 ctgccggaat attattgtgt acgttatgtt tactgcaatt tattgtcagc ctgatgatcg
     2341 agaatcgcta tgagcataat ctgacttcat cgcttttctg gattatttgg ttcccggtta
     2401 ttttctggat gctgagcctg gcaacgacat tggtatcatt tacacgagtc atgttgatgc
     2461 ctaaaaagca acgcgcccgt tgggtaagtc ctgatcgcgg gattctgaga ggttaatatg
     2521 aacaatttaa ttattacgac ccgacaatca ccagtacgtt tactggttga ttatgttgcc
     2581 acaaccatct tgtggacatt atttgctttg ttcatattct tattcgccat ggatctgctg
     2641 acgggttatt actggcaaag cgaggccaga agccgacttc agttctattt tttgctggca
     2701 gtggcgaatg ccgtcgtgtt aattgtctgg gcgctgtaca ataagctgcg ttttcaaaaa
     2761 cagcagcatc atgcagccta ccaatatacg ccgcaagaat atgcagagag cttagcaata
     2821 cctgatgagc tctatcagca actacaaaaa agccacagga tgagcgtaca cttcaccagc
     2881 caggggcaaa taaaaatggt tgtttcagaa aaagcgctag tccgggcata aacacccaaa
     2941 acaaagcccg gttcgcccgg gctctgcacc gataatacac ttaactgtat gcatgcagcg
     3001 tacgttggca aagtgccgaa cgtacgcaat cctcttttcc gaaccggacg atcccaacca
     3061 tttcatcttc ttcgaaacgt tccagcgcgt cacttaatcc ggagcacacg ccgcgaggca
     3121 aatcgcattg cgtgatatca ccgttgacga taaccgtcac gttctccccg aggcgggtta
     3181 aaaacatttt catttgcgcg gcagtcacat tctgcgcctc gtcaagaatg acgactgcat
     3241 tttcaaaggt acgtccacgc atataggcga acggcgcggt ttccaccttc cctatttccg
     3301 gtcgcaggca gtactgcata aaggaagccc ctaagcgccg gaccagcacg tcgtagaccg
     3361 ggcgaaaata gggagcaaac ttttctgcga tatcaccagg taagaagcca agatcttcat
     3421 cggcttgcag aactggacgg gtgacgataa tcctgtcgac atccttatgt atcagggcct
     3481 ctgccgcttt tgctgcgctg atccaggttt ttccgcaccc ggcttcgccc gtggcgaata
     3541 tcagctgctt actctcaata gcctgcagat agtgcaattg cgcttcattt cgcgcaagga
     3601 tgggcgaggt atcgcgactg tcgcgggcca taccaatggc ttctacgccg ctcatctgca
     3661 caagcgaggt gaccgattct tcttcacgct gcttatggct gcgcgaatcc cgtctcagca
     3721 cacgttttgc ctcgcgacga gctttgatca ctgctttttg tcttcccatg gagagcacct
     3781 tgagttgttt gtattcatca cacgcgccgt tggcagcgcg attatgcaca cgaacatcag
     3841 agggttggct tccttgtaag ccatagtttg cttttggata aaatgccgaa aaacggctac
     3901 gcacaccgtt tgcggcgtcg gtaacacgag taaagaaagg aagtgagaaa aatacagagt
     3961 gacgaagttg ctaccggagg agaaatctcc gcgagtggtg gaacgttgct taacaaaaca
     4021 ttgtccagaa caggacgtta ccatccgcga tctccatagt gactgactat cactgccggg
     4081 aacttccgct gctacttaat aagtacaaca gaactcgcat tcattgcaac aatatattca
     4141 cttatattta acgataaata cccatttcgg tgacattaat ttctactgga aagatgacag
     4201 agtgatgaca gtgatggaaa aagctgtgtg ctttccgcag gattcgcagg gctggtaaaa
     4261 tgtgcctcgt gtttgccgga ttcggcgcga acgccttacc cgacctacaa gtgattaata
     4321 atttgtgcac aaaactcatg cccgataaac ctgcgcatcg ggcatatttg cgtcgttcaa
     4381 acctgcaata gcgcccttcc cagataatcg ttcgcgtcct tcactcccgg cagcgaaaaa
     4441 aaataaccgc cgccgatagg tttaacgtat tcctccagcg cttcgccatt gagccttttt
     4501 tgtactgtca ggaagccttt ttccagatcg tgttggtagc agacaaacag caaccccata
     4561 tccagttgtc cggagttggt gacccccaac gaataactgt agccgcgacg cagcatcagg
     4621 ctggactcac tctccgccgt gcggggattc gccagccgga tatggctgtc cagcgcgatc
     4681 accttccctt ccgggtcgct ggcgtaatca ggcacatcat gctcatgctg cattcccagc
     4741 ggcgcaccgg tttgcttatc acggccaaaa atcgtctgct gttctttcag cggcgttctg
     4801 tcccaaaatt ccactcgaaa ctgaatcaag cgtactgcct gatagctgcc accgattgtc
     4861 cacgcaggct cctgctgatc tgccgttacc cacaccactt tttgcatcaa cttatcattc
     4921 tggctatcgg gattggcagt gccgtctttg aaacccagca aattaatcgg cgtctctttg
     4981 cctttactac gcgccgcgtg atcggaaata aacccttccc gcttccagcg cacactgagc
     5041 aaatccggcg tgtgtttgat gatatcgcgc agcgcatgga tcaccgtgtc ctgggtgttg
     5101 gcgcaaatct gtagcaacac atcaccatga cataacgccg catccagcga gtcgttgggg
     5161 aaacgcgtca tcttctgcag cttttttggc atctgtggcg caaggccaaa gcgctcatca
     5221 aacaatgagt gacccaccga taacgtgatg gtgagattat cgggcgcaat gtagccgcca
     5281 agaatgccgg aatcgagtgg tggcaggcgc ggattcggcg tttctggtgc tgctccgccc
     5341 tgactcagaa aagcaaaacg ctgagtcaac aagcgaaaca accgctcaag atcggcttta
     5401 tcgctggcaa gcacatcaaa cgccaccagc atcattgcgg cctgttgtgg cgtcaggatc
     5461 cctgcctgat gctcaccata aaacggctgt ttctcattgc gtgcatccgg tgaaagcgtg
     5521 cccggtgcac tttgcgtttt ttgtgcatga gcgaccggac aacttcccgc cagcgcacca
     5581 atccctttca gtaaacgtcg gcgtgacggt tcattcacgc cgttttcatc tttatactgc
     5641 ataacgctta atccagtccc agcacaccgc gaagttgcgc cagatcttcc gccagcgcag
     5701 taatcggccc tttcagcgca ttccggtcgg catcggtcaa tttgtcgtag gtttcaaaac
     5761 cgtctttagt acggtatttc gccagaatgg tatcgacctt tttaaagttg gcatcgactt
     5821 ttgccaacag ttccgggttg gctttttgca gttgtggacg cagcagatcg acaattttct
     5881 gcgagccttc aacgttagcc tggaaatccc acagatcggt gtggctgtag cgatcttctt
     5941 caccgctgat tttgctggct gccacttcct caatcagtcc ggctgcgccg ccgaccactt
     6001 ttgaaggtgg gaaagccagt tcactgatgc gtttttgcaa atcgaccaca tcggtataaa
     6061 gctgctcagc gtactgatcc atccctttgg tggtgttgtc gccaaacaat gctttttcca
     6121 gacggtggaa accagtgaat tttgggtcgg cggctttttg ctcgtaatca tcttcacggg
     6181 cgtcaatgct gccatccaga tcggagaaca gttcagcaat cggttcaata cgctcatagt
     6241 gctggcgcgt cggcgcatac agtgctttcg ctttttcgat atcgcctgct ttaatcgcgt
     6301 cggtaaaggc tttggtgtcg gtcaccagct gcgtggtttc cgccatgaca tacgctttat
     6361 atgcagtaat tgcaccacca agacttaaca gcgcatcact ttgcgccgca tccgccgttg
     6421 cctcaccttt gacgatcaac ttccctttcg ggttagtcag cagaccgcag gtcatatcgt
     6481 attcgccagg ctgtaaattc gccgtcattt tctggctaaa accaggggcg atattttccc
     6541 gctcttccac caccatcacg cctttgagga tctcccactc cagcgccttc tggctatggt
     6601 tctgaataat gaactgtgtt ttcccggcgt taaccgtaat ggtcatcggt tcgcactgct
     6661 tatccgtcac ggtcactttg acctgcggca catcagcggc gttagccata aaagcagaag
     6721 aaaacagcgc agccacgctc aactgcaatg cgttacggcg gaagttaatg gtcatgacga
     6781 gactatccct ttaaagtatg ttgtaactaa gcaagagttt gcgtcgtatt tgttacgcgg
     6841 agcgagacgc tgtcgcccct gcgcgtggtg gcagagcaaa tgccaccagc gccgggatga
     6901 gataaataaa ccagacggcg acttcgctga cgctcggtgc ttcctgatag ccaaaaatcc
     6961 cttccatcag cgtgccaaac agcgagtgag ttgagaggac cgcgctcata tcgaaggcga
     7021 tttcctgaaa gtggttccac aatccggctt catgaaatgc gcgaatggca ccagccgcca
     7081 gccctgcggc gacgaagaga ataaacaggc tggtccattt aaaaaatgca ccaagattga
     7141 ggcgaatacc gccccagtag agcaggaagc cgagcaccac ggcagtagca agaccgagca
     7201 ttgcacccag cggcggccag atcccgacat cttgttgaaa tgccgccagc aggaaaaaga
     7261 ccgactccag cccttccctt gcaacggcaa aaaagaccat catcaccagc gcccagccat
     7321 gatgatttcc acgctgcaat gcgctatcga ctgcctgttc cagttgcact ttgacgttgc
     7381 gcgacacttt gcgcatccag aaaaccatcc aggtaaggat caccacggcg atcaccgcca
     7441 cgataccttc aaacagttcc tgctcttttt gcggaaattc gccggtggtt tcgttaatga
     7501 agatcc
//
LOCUS       DADLSA010000170         7494 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.171, whole genome shotgun sequence.
ACCESSION   DADLSA010000170 DADLSA010000000
VERSION     DADLSA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.171"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..544
                     /locus_tag="JN691_003586"
     CDS             <1..544
                     /locus_tag="JN691_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HAY7244916.1"
                     /translation="RYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVLDQPTM
                     KALDKQNVAQALSVVPGVVLQKSGSRNEKQVKVRGFDSRQVPVYFDGVPIYVPYDGNL
                     DLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVRTMPTI
                     CMLHLPPAAILGICKSAVAS"
     gene            481..1941
                     /locus_tag="JN691_003587"
     CDS             481..1941
                     /locus_tag="JN691_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY7244917.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKE
                     SFYYQGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYFDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKEGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(1991..2245)
                     /locus_tag="JN691_003588"
     CDS             complement(1991..2245)
                     /locus_tag="JN691_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY7244918.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            2446..3180
                     /locus_tag="JN691_003589"
     CDS             2446..3180
                     /locus_tag="JN691_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAY7244919.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYNLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(3182..3793)
                     /gene="emtA"
                     /locus_tag="JN691_003590"
     CDS             complement(3182..3793)
                     /gene="emtA"
                     /locus_tag="JN691_003590"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAY7244920.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            3893..4807
                     /gene="ldcA"
                     /locus_tag="JN691_003591"
     CDS             3893..4807
                     /gene="ldcA"
                     /locus_tag="JN691_003591"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAY7244921.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            4902..6638
                     /locus_tag="JN691_003592"
     CDS             4902..6638
                     /locus_tag="JN691_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAY7244922.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            7249..>7494
                     /locus_tag="JN691_003593"
     CDS             7249..>7494
                     /locus_tag="JN691_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244923.1"
                     /translation="MASFKDICLFAPAIPPSSVNPGVRRPPGRLFLSVELKNQILQNM
                     KYLSPQKFSWGDAPWQIIDLSIAGKVNIQVDNNTIITL"
BASE COUNT         1820 a         1910 c         1905 g         1859 t
ORIGIN      
        1 acgttatctc tgcacagcat tatcacttgc ctttacccag caggccgtag cggctcagga
       61 gagcgacacg ctgaccgtat ggtccagtcc ggtatcatcg acgacgacca ccgttctcga
      121 tcaacccacc atgaaggcct tggataaaca gaatgtcgct caggcattaa gtgtcgtccc
      181 cggcgtggtg ctgcaaaagt caggcagccg caacgaaaaa caggtaaaag ttcgtggctt
      241 tgatagtcgg caggtgccgg tctatttcga cggtgtgccc atttatgttc cctatgacgg
      301 caacctcgat ctggcgcgga ttctgaccaa caatctgggg gcagtcgaag tttccaaagg
      361 gtattcgtcg ctgcttcatg ggcctaatca gatgggcgga gccattaata tcaccaccct
      421 gaagaagcaa gtctgggata tcgccaggga tggagccgta gtcaggacaa tgcctacgat
      481 atgcatgctt catttgccgc cagcagcgat cttgggtatt tgcaagtcag cggtagccag
      541 ctaaagcagg attttctcgg cctgccgcat ggtgtaaata atgatattgc aggcaaacac
      601 ggcaagatga ttaattcatc ggctgatgat aaacgcggca ttgtgaagct cggttttaca
      661 ccgcgcgaaa acgatgaata cacgctgact tacattaagc aggatggtga aaaagataac
      721 ccgccataca gcggaaatag tggtcaaaaa tcacgctact ggcagtggcc agagtatgac
      781 aaagaaagtt tttattatca gggaacgacc caactaaacg accgttttac cctgaaaagt
      841 cggctgtatc gcgacacctt tgaaaatacg ctgatgatgt acaactcgct ggctgatttg
      901 aaaaataaaa aaggcagcta cagccattat ttcgattaca gcgacggtgc cggattacaa
      961 ctggcagccg atgtgcgtga aaacgatctg ctgtcgtttg ccgttaactg gaaagatgac
     1021 gtacaccggg aaaaaggtgc gccgcacgcc gcttacgatc gctatgaaga tcgtacctgg
     1081 tcgctcgcca gtgaatatca atgggctgct gctgataatg tcgatgtcgt ggcgggaatc
     1141 agctatgact ggcgcgatag cgtagaagcg aaaaaacacg agaaagaggg cagcatcacc
     1201 cattatgacg acaacaatca gtcagctttt aactggcagg tgatggggaa ataccacttt
     1261 gccaatgaag acactctggc gctttcgtac tatgaccgca cacgctttcc gacgctgaaa
     1321 gaacgctata ccacatccaa acctgcgtat aaccagatag cgattgttaa cccgcagctc
     1381 aagccggaac gcgctcgcgg ggtggattta acctggaatg gtgccttcac gcgcgactgg
     1441 ggatttgagg tcagcgttta ctataaccgg gtgagtgatg ccatcctctc gcacaatatc
     1501 aatgccgata ccattcaaaa tcagaacagc ggcacggtgg attacagcgg tctggatgcc
     1561 ggtattaagg ggaaaatcag caatatactg gatgtaggat tgagctacgc tctgatccac
     1621 gctgacgcca aacgcaaaga catcggcaag ataaccgatc ttccaacgca gacaatgacc
     1681 gcatggatga cactcaaacc atgggagccg ttaagcgtaa cgctgtcgga agaggcgcgt
     1741 tcctccagct acagcaacag tgacggttca caaaaagccg ccggttttgc ggtgacccac
     1801 attcgagccg attacacctt aggtcatggc ttcagcgtca atgcgtcggt caataacctg
     1861 tttgataccc aatatgccta cagtgaaggg tttattgaag aaggtcgcaa tttctgggca
     1921 ggtgtcgagt atacgttctg atgtgaaagc ggatagcggc atctgtatcg ctatccgcct
     1981 tatgaaaatc ttattctctt cgcaggaggc ggaatacgcc cagcaccagg atagcgccca
     2041 ccaccgccac caggaagctg tgcaaattaa aaccgctgat ggagccgcca atgccaaaca
     2101 tggtcgccag ccagccgccg accaccgcac cgactatccc gagaatacag gtcaggaaaa
     2161 atccaccacc atcacgcccc ggcatgatta gcttggcgat aatgccggct atcaggccaa
     2221 aaataatcca ggcaataatt cccatgttca ggccctcgtt tcgatgaatc ggtcgcaaga
     2281 aatgcttacg tcaacgctct aagtataggc aagggcggga tcgatgctgg gcgtttgtcg
     2341 gctgatttaa gaacaacatg taaaaaatat tcacgcattt gtattaagtt ttgttaacta
     2401 tgaccgataa accaaagaca gtttgtcagt caggagtttt tccgcgtgag tcattaccat
     2461 gagcagttcc tgaaacaaaa tccgttagcc gtcctgggcg tgttacgcga tttgcacaaa
     2521 gccgcaattc ctttgcgtct cagttggaat ggcgggcagc tgatcagcaa aatactggca
     2581 ataaccccgg ataaactggt gctggatttc ggcagtcaag ccgaagacaa catcgccgtg
     2641 ctaaaggcac agcacattac cattaccgcc gaaactcagg gtgcgaaagt cgagtttact
     2701 gttgaacaac tacagcagag tgaatacttg cagcttccgg catttattac cgtaccgcct
     2761 cccaccttat ggtttgtaca acgacgccga tatttccgca tctccgcccc actccatccg
     2821 ccttattttt gccagaccaa actggcggat aacagtacgt tacgtttccg cttgtataat
     2881 ttgtcgttag gcggcatggg cgcattactg gaaacagcaa agcctgccga attacaagaa
     2941 ggtatgcgct tcgctcaaat tgaagtcaac atggggcaat ggggtgtttt tcactttgac
     3001 gcccagttaa tctccaccag cgagcgcaaa gtgattgatg gcaagaatga aaccatcacc
     3061 actccccgtc tgagcttccg ttttcttaac gtcagcccga cggtggagcg gcaattacag
     3121 cggattattt tctctctcga gcgagaagcc cgggaaaaag cggacaaagt gcgcgactga
     3181 tttacatcgc gtccagtgcc tgctcaagtt tgtagatata gcgcggagcc tgcggcgcag
     3241 gatgatttcg cgctacgtga tcgaggaact cgtcagcatc caaatcgttg attttgctga
     3301 tcgccttttt ccgatctgac gagaaagtcc gtagcagcgc acctgccccg ttagcgtatg
     3361 acaccaccag cgcatattgc agtaccttcg gatcttcaat gcctgccagc gggccggttt
     3421 ccagaatatt taggtaagcc gcccccattg aaatattacg ctctggattt ttcagctcgc
     3481 tggtcgtcgg ctcaccactc cagcccatac ggcgataaac atcacgtccg gaggttgaag
     3541 cttttaactg catcaaacca atggcattcg atttactcac cgcgttcgga ttaccacccg
     3601 attcgatagc gataatcgcc gtgatcaatt gtggatcgac gccccaggct gcaccggctt
     3661 tctggcttat tggcatccac tgcatcgcac gttgcaccgg aactttcgcg ttccacggcg
     3721 ggttcgtata gtcatgcttt gatgaacaac ccgctaataa cacaatcaaa aaggcaaacc
     3781 atctcaattt cacttcttct atccttatag cctggaatcg ccgttgaggt gacaacggca
     3841 cacctaagcg gcatgatatg catttcgttt ctgttgtcag caaggaattg ccatgtctct
     3901 gtttcactta attgccccat cgggttacag cattaaacag cacgccgcgc tgcgtggtat
     3961 ccagcgcctt accgacgcgg ggcatcaagt caataacgtc gaggttattg cccgtcgttg
     4021 tgagcgtttt gcgggtacgg aaacggagcg tctggaagat ctcaattccc ttgccagact
     4081 gactaccccc aacaccatcg tgctggctgt acgcggcggt tacggtgcca gtcgtttact
     4141 ggcagatatt gactggcagg cactggtggc tcgccaacaa catgacccgt tactcatttg
     4201 cggacatagc gattttaccg ccattcagtg cggtcttctg gcgcaaggca atgtcatcac
     4261 ctttagcggc cccatgctgg tagcaaactt tggtgcggat gaactgaacg cctttaccga
     4321 gcaccatttc tggctggcgt tacgcaatga aaccttcacc attgaatggc aaggggaagg
     4381 cccgacatgt cagaccgaag gcacgttgtg gggaggcaat cttgcgatgc tgatttcact
     4441 gattggtaca ccgtggatgc caaaaattga gaacggtatt ctggtgctgg aagatattaa
     4501 cgagcaccct ttccgcgtcg aacgtatgct gttgcagctc tatcatgcgg gaattttgcc
     4561 acgtcagaag gcgattattc tcggtagctt tagcggcagc acgcccaatg attatgacgc
     4621 aggttacaac ctggagtcag tgtacgcgtt tttgcgttcc cgcctgtcga ttccactcat
     4681 taccggcctc gattttggtc atgaacaacg cacggttacg ctgccgttgg gcgcacatgc
     4741 cattctgaat aacacccggg aaggcactca gttgacgatt agcggtcatc ctgttcttaa
     4801 aatgtaaaaa aatcacgctt cagggctaag taaaacgctg tctctgccgc taatatggta
     4861 gggtttatat aagagacagc gtaatcagga gtaaccgacc gttggatgcc acaacaataa
     4921 ttagcctttt catcttagga tccatccttg tcaccagcag tatattactt agttcatttt
     4981 cttcccgtct tggcattcct attctggtta tttttctggc gatcggcatg ctggcgggag
     5041 tcgatggcgt cggcggtatc ccgtttgata attacccctt cgcctacatg gtaagtaacc
     5101 tggcgctggc gattattttg ctcgacggcg ggatgcgcac tcaggccagt tcctttcgtg
     5161 tggcgttagg accggcgctg tcgctggcga cgctgggcgt acttatcacc tctggtttaa
     5221 ccggcatgat ggcggcgtgg ctgtttaatc ttgatttgat tgaaggctta ttaatcggcg
     5281 ctatcgtcgg ctccaccgat gctgcagcgg tcttttctct gctgggtggt aaggggctta
     5341 acgaacgtgt tggctcgacg ctggaaattg aatccggcag taatgatcca atggcggtct
     5401 ttctgacgat taccctgatt gcaatgatcc agcagcatga aagcagtgtc agttggatgt
     5461 tcgtggtcga tattctgcag caatttggcc tcgggattgt catcggtctt ggcggtggtt
     5521 atttactgct gcaaatgatt aatcgcatcg ccctgcctgc cggattatat ccattgctgg
     5581 cattaagcgg cggtatttta atttttgctt taaccacggc gctggaaggc agcggtattc
     5641 tggctgttta tctgtgcggt tttctgctgg gtaatcggcc gattcgcaac cgctacggca
     5701 tcctgcaaaa tttcgacggc ctcgcctggc tggcgcaaat cgccatgttc ctggtgctgg
     5761 ggctattggt taacccaagc gatctgctgc ctattgccat tccggcgctc attttgtcgg
     5821 catggatgat attcttcgcc cgtccccttt cggtatttgc tggattgctc cctttccgcg
     5881 gtttcaatct gcgtgagcgc gtgtttatca gctgggtggg attacgcggc gcggtgccaa
     5941 tcatcctggc cgtgttcccg atgatggcgg ggctggagaa tgcgcgactg ttctttaatg
     6001 tcgccttctt tgtggttctg gtttcactgc tcttgcaggg aacatcactc tcgtgggcgg
     6061 cgaaaaaagc caaagtggtg gttccgcccg tcggacgtcc ggtgtcacgc gttggcctgg
     6121 atattcatcc ggaaaatccg tgggagcagt ttgtttatca attgagtgct gataaatggt
     6181 gtgtaggtgc ggcactgcgc gatttgcata tgccaaaaga gacgcgtatt gcagccctgt
     6241 ttcgtgataa ccagttgctt catcctaccg gcagcacccg actgcgcgaa ggcgatgtat
     6301 tgtgtgtaat tggtcgggaa cgcgatctcc cggcgctcgg taaactgttc agccagtcgc
     6361 caccggtcgc gctggatcaa cgcttctttg gtgacttcat tctcgaagcc agcgccaaat
     6421 atgctgatgt tgcgctgata tatggcctgg aagacgggcg agaatatcgt gataaacagc
     6481 aaacgctggg tgaaattgtc cagcagttgc taggcgcggc accggttgtc ggtgaccagg
     6541 tagagtttgc cgggatgatc tggacggtgg ccgagaaaga agataatgaa gtgttgaaga
     6601 ttggcgttcg ggtggctgaa gaggaagctg aatcttaaat ttaatgcagc atctatcgcc
     6661 ggatgcgatg ctttactgaa cgcagcctga gcctgtaata aacttgtcat ttcctgattt
     6721 tgatatgctc atcccaacat cagaagaagg ttaaattatg gatgacaaac cattgcaggc
     6781 tctggctaat gaactcaaaa caccggctca agaaagtcag cgtaagggct ttttccgact
     6841 gtaacaggta ataaaaacca gccccgcatt accggtcaac catcaggggc ccctgcctga
     6901 catcgcctga acatattccc ccacgcaacc tgccgcactg acatagccca cacaataggc
     6961 gtttcatccg cctgtagcac cggatgcatg agcgatttct tatagttatt cctttgctta
     7021 ataacgatta tttccgggtc gttcactctc gtattttctt taatacgtca gagctaccta
     7081 ggccgtaata cagcatgtac cagacccgac acggcatttc tgaccaggga aaaagtcaac
     7141 gatgagtagt gatatcagcg caaatgacgt cctgtggctc ctttgcggtg accacattca
     7201 cgtctccggc gctgccagag gatatttatt tcaaggaaac gtagcattat ggctagcttt
     7261 aaagacatct gcctattcgc ccccgccatt cccccatctt ccgttaaccc aggcgtacga
     7321 cgcccgccag ggcggctctt cctgtcggtg gaactcaaga atcaaatttt gcagaacatg
     7381 aagtatctca gcccgcaaaa attcagctgg ggtgatgccc cctggcagat cattgacctc
     7441 agtatcgcgg gcaaagtgaa catacaggtg gacaacaata ccatcatcac gttg
//
LOCUS       DADLSA010000171         7491 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.172, whole genome shotgun sequence.
ACCESSION   DADLSA010000171 DADLSA010000000
VERSION     DADLSA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.172"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(76..357)
                     /locus_tag="JN691_003594"
     CDS             complement(76..357)
                     /locus_tag="JN691_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HAY7244924.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(619..1728)
                     /gene="apbC"
                     /locus_tag="JN691_003595"
     CDS             complement(619..1728)
                     /gene="apbC"
                     /locus_tag="JN691_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAY7244925.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1860..3893
                     /gene="metG"
                     /locus_tag="JN691_003596"
     CDS             1860..3893
                     /gene="metG"
                     /locus_tag="JN691_003596"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAY7244926.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERTEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4034..7491
                     /locus_tag="JN691_003597"
                     /pseudo
     CDS             4034..7491
                     /locus_tag="JN691_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
BASE COUNT         1944 a         1946 c         1807 g         1794 t
ORIGIN      
        1 tatcaataaa aaagacgaaa tcatttatat aaatgatttc gtctttctaa atgcaattaa
       61 aatcaggttg ccaaattaat cgtagcgatt taacacgatt cgacgtttgc tatcgctacc
      121 tgcaacaacc acttctgtcg taacaccttt gacgggcgaa gaaacctgat gacgggtgtc
      181 aacattatac gttaactgac cacaatgctg ttgtggttga ctattaacca tagccatact
      241 gcatgttggg ggactgatcc tgccattaat cgttaatgtg gcagtctctg aagagactgc
      301 gggagaagcc aatccatagg caagaaaaat aattcctgac agccaatact tattcattga
      361 acgttatccc tgtagtaaag gttatgcctg gatagaatga gtgcataaca aactatagct
      421 gtacatccac tacacagcac gaaggatgat aatgaagcat tgcctgtacg atcactcgac
      481 tttgtagagt ttctgacgaa ggtccgcaga atattcgcag tattaaataa gtgttcatta
      541 atgcggcaaa aacaaataca ccgcatcaat ataacatctc tgcggtgtat gaagaataac
      601 cagaatggtt aataggcgtt agaccgcgcg gaaggaaatc tcgcctggaa tgacttcacc
      661 ctgccagtag agctgagctg caacgcggtc agccagctgg cggtagatag cggtaaattc
      721 gctctccgga cggctgataa ccgttggcgt gcctttatcg agatcctcac gtaaggagat
      781 gtgtaacggc atctgcccca gcagctgtgt atggtatttt tcagccagtt tctctgcacc
      841 accggtaccg aaaatcggtt catggtgacc acagttactg caaatatgca cactcatgtt
      901 ctcgacaata ccgagcaccg gaacctcgac tttctcgaac atcacaatgc cttttttcgc
      961 atcgatcaac gcgatgtcct gcggggtggt gaccacgact gcaccagtta ctggaatgtt
     1021 ttgcgccagc gtcaattgaa tgtcaccggt gcctggcggc atatcaagca ccagatagtc
     1081 gagatccggc cacaaggttt cctgcaacat ctgcattaac gccttgctcg ccattggtcc
     1141 acgccacacc atagcattgt cgtcagtgac cagataacca attgaattgg ttgccaggcc
     1201 atgagacata atcggtgcca tgtgggtacc gtccggcgaa gttggacgct gattttctgc
     1261 gcccagcatg gtgggaattg atgggccgta gatatcggcg tccagaatgc cgactttcgc
     1321 cccttccgcc gccagtgcca gcgccaggtt taccgccgtg gacgatttac ccacgccgcc
     1381 tttaccggaa ctcaccgcga taatgttctt cacaccgtta attcccggct gattcttcac
     1441 gcgtttcaga gtggctatgt tatgcgacag tttccagtcg atagctttcg ccccggtgat
     1501 gcgcagcagt tcggcgctac attgctcttt taactcttcg aacgcgctat gccagacaaa
     1561 cggcataacc agttccacat gcaacgtgtc gtccatccag gcgacatggt gcaacgcttt
     1621 cagcgtggtg aggttatgct tcagggttgg gtgctgaaaa ttggccagtg tcccggcgac
     1681 cattgcgcgc aaggcttctg gtgatttggc ctgggattgt tcgttcatcc cgactccttt
     1741 tttgtataga taaaccatca gctgatagtt tacctgaaga atatagagaa gtacttactt
     1801 aacattttcc catttggtac tatctaaccc cttttcacta ttaagaagta atgcctacta
     1861 tgactcaagt cgcgaagaaa attctggtga cgtgcgcact gccgtacgct aacggctcaa
     1921 tccacctcgg ccatatgctg gagcacatcc aggctgatgt ctgggtccgt taccagcgaa
     1981 tgcgcggcca cgaggtcaac ttcatctgcg ccgacgatgc ccacggtaca ccgatcatgc
     2041 tgaaagctca gcagctcggt atcaccccgg agcagatgat tggcgaaatg agtcaggagc
     2101 atcagactga tttcgcaggc tttaacatca gctatgacaa ctatcactcg acgcacagcg
     2161 aagagaaccg ccagttgtca gaacttatct actctcgcct gaaagaaaac ggttttatta
     2221 aaaaccgcac catctctcag ctgtacgatc cggaaaaagg catgttcctg ccggaccgtt
     2281 ttgtgaaagg cacctgcccg aaatgtaaat ccccggatca atacggcgat aactgcgaag
     2341 tctgcggcgc gacctacagc ccgactgaat tgatcgagcc gaaatcggtg gtttctggtg
     2401 ctacaccagt gatgcgtgat tctgaacact tcttctttga tctgccctct ttcagcgaaa
     2461 tgctgcaggc atggacccgc agcggtgcgt tgcaggagca ggtggcaaac aaaatgcagg
     2521 agtggtttga atccggcctg caacagtggg atatctcccg cgacgcgcca tactttggtt
     2581 ttgaaattcc gaacgcgccg ggcaaatatt tctacgtctg gctggacgca ccgattggct
     2641 acatgggttc tttcaagaat ctgtgcgaca agcgcggcga cagcgtaagc ttcgatgaat
     2701 actggaagaa agactccacc gccgagctgt accacttcat cggtaaagat atcgtttact
     2761 tccacagcct gttctggcct gccatgctgg aaggcagcaa cttccgcaag ccgaccaacc
     2821 tgtttgttca tggctatgtg acggtgaacg gcgcgaagat gtccaagtct cgcggcacct
     2881 ttattaaagc cagcacctgg ctgaatcatt ttgacgctga cagcctgcgt tactactaca
     2941 ctgcgaaact ctcttcgcgc attgatgata tcgatctcaa cctggaagat ttcgttcagc
     3001 gtgtgaatgc cgatatcgtg aacaaagtgg ttaacctggc ctcccgtaat gcgggcttta
     3061 tcaacaagcg ttttgacggt gtgctggcaa gcgagctggc tgaccctgag ctgtacaaaa
     3121 cctttaccga tgccgctgaa gtgattggtg aagcgtggga aagccgtgaa tttggtaaag
     3181 cagtgcgcga aatcatggca ctggctgacc tggctaaccg ctatgtcgac gagcaggctc
     3241 cgtgggtagt cgcaaaacag gaaggtcgcg atgctgacct gcaggcgatt tgctcaatgg
     3301 gcatcaacct gttccgcgtg ctgatgactt acctgaagcc ggtactgccg aaactgaccg
     3361 agcgtacaga agccttcctc aatacggaac tgacctggga tggtatccag caaccgctgc
     3421 tgggccacaa agtgaatccg ttcaaggcgc tgtataaccg catcgatatg aaacaggttg
     3481 aagctctggt ggaagcctct aaagaagaag tgaaagccac taccgcgcca gttactggcc
     3541 cactggcaga tgatccgatt caggaaacca tcacctttga cgacttcgcc aaagttgacc
     3601 tgcgcgtggc gctgatcgaa aacgcagagt ttgttgaagg ttctgacaaa ctgctgcgcc
     3661 tgacgctgga tctcggcggt gaaaaacgca atgtcttctc cggtattcgt tctgcttacc
     3721 cggatccgca ggcactgatt ggtcgtcaca ccattatggt ggctaacctg gcaccacgta
     3781 aaatgcgctt cggtatctct gaaggcatgg tgatggctgc cggtcctggc gggaaagata
     3841 ttttcctgct aagcccggat gccggtgcta aaccgggtca tcaggtgaaa taatccccct
     3901 tcaaggcgct gcatcgacag cgccttttct ttataaattc ctaaagttgt tttcttgcga
     3961 ttttgtctct ctctaacccg tataaatact ggtagcatct gcattcaact ggataaaatt
     4021 acagggatgc agaatgagac actttatcta tcaggacgaa aaatcacata aattctggac
     4081 ggtggagcaa cagggaaacg agttgcatat cagttgggga aaagttggca ccaaagggca
     4141 aagtcagata aaaagttttt cagatgctgc ggcggcggaa aaagcggaac tgaagctgat
     4201 tgcggagaag gtgaagaagg ggtatgtgga gcaagcgaaa gataattctt tgcaaccttc
     4261 ccaaacggta acggactctc tcaaggtagc ggacttatcc accattattc aggaacaacc
     4321 ctctttcgta gcagaaaccc gtgcggctga caaaaataca gatgctgttt taccttggct
     4381 ggcgaaagat attgctgtcg tttttccgcc cgaagttgta cacaccacgt taagtcatcg
     4441 ccgctttccc ggagttcctg ttcagcaagc agacaaattg acccaattac gtcgcttagc
     4501 ctgtagcgtg tcgcaacggg ataataaaac agccacattt gacttcagca cctgttcttt
     4561 agaatggcaa aacaccgtcg cccaagcgat cagtcagatc gacggcctga aaacaacaca
     4621 gctaccatca ccagtaatgg ctgtactcac ggcacttgaa atgaaaccag gaaggcggtc
     4681 tggaatatgc tactgatgta ataatacacc ttcaacagat tgatattaaa tgggattatg
     4741 cgaataatgt cattattatt ctgccgtctg gcattgcacc tgactacttg gagcaatatt
     4801 ccagatttga attacgccta cgtaaacatt tatcactggc ggaagagtct ctctggcaaa
     4861 aatgtgcaca aaaacttatt gccgcaattc cacatattcc agaatggcgg caaccattaa
     4921 ttgctttgtt attacccgaa aaaccagaaa ttgcacatga aattgcccag cgtctactgg
     4981 ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt ggcgactgat gagcacattc
     5041 ttgcctcatt agaaaaatat cacgagccat atgccatttt tgatgattac tattgtggtg
     5101 cgatatggtc agccaccgta ttacaggagc aaggtgttgc agccctgccc cgatttgctc
     5161 cctatgccgc aagtgactac tgcgccgatg tgttgcgtca tatcaatcat ccgttcgcat
     5221 taacactgct tatacgtgta gccgggcata ctaaacgctg tcacgatcga atgacgaaag
     5281 cctgcgctgc gttcccacac gcagcactgg cagcactggc ggaacttcta gtgcaaaaag
     5341 aagagaatag ttggcgcatt atgctaatga cgatgtttat ctcgcaacca gcactggcag
     5401 aacaggtcat tccctggctt tcgacacccg cagttgcagt gctgaaatca tgccagcacc
     5461 aactgacaca gccctcaaac cataccagcg ccgatctact gccagccata gtagtctccc
     5521 caccatggat ttcgaaaaag aaaaaatcgc cgattccggt gctggattta gcgccattag
     5581 gcatcgagcc aatctgttat ctgacagaag aaatcagtaa tcaacttttg gcgaaatata
     5641 tctggtattc aaaacacatc acggttagcc atgaagaaag tactaccaac ctgttggcaa
     5701 ggatgggttt tcaacgacgg atcgctggta catatattaa agctcccgaa gcggtagttg
     5761 aggcatggct aaatgaagat tattcaacct tactaagtga atttaaggtg tttcattcac
     5821 ctaccgggca ttattgacag ttggggattt tgacaacatt gccgctggag gctggagaaa
     5881 gcagtaaaag catggaatgc ccttactcta tctccacata ccgataccga atacgccatg
     5941 ttacattttg gactcaaagg tttacctggt ttagttaact caattacacg ctatccgcag
     6001 gaagccttgc ccatcacgaa ttacttcgca gcgagtgagc tggctcctgc cgtcgcccgt
     6061 gccttcaaca aactcaaaac gctacgcgaa aacgcccgca cctggctgtt gaaatacccg
     6121 gaacatgccc ttaccggcct gctgcctgcg gcgctcggca aagccggtga agcacaggat
     6181 aacgcccgcg ctgccttgcg tatgcttacc gaaaacggtc atcagccatt actgcaagaa
     6241 atcgcccggc gttataacca gtcggaagta acagacgcgg tgaacgctct gcttgcgctc
     6301 gatcccttag ataatcaccc gacaaaaatc cccactcttc cgacctttta tcagccatcg
     6361 atctggacgc gcccggtatt aaaagcaaac gcccaatcac tgccagataa cgccctcctc
     6421 cacctcggcg aaatgctccg cttccctcag gaagaggctc tgtatccggg attattgcag
     6481 gtgaaagacg cctgtaccgc tgactctctg gctgaatttg cctaggatct gtttaccgcc
     6541 tggcagaccg ctggcgcgcc gtcgaaagag agttgggcgt tcactgcgtt aggcgttctc
     6601 ggtaacgatg acaccgcccg caaactgacg ccattaatac gcgcctggcc tggtgaatcc
     6661 cagcataaac gcgccaccgt tgggttggat attctcgccg ctatcggtag tgatatcgcc
     6721 ctgatgcagc ttaacggcat tgctcagaaa ctgaaattca aagcattaca ggagcgggca
     6781 aaagaaaaga ttgccgacat tgccgagagc cgcgaactca cggtagcgga gcttgaagat
     6841 cggttagcac cggatctcgg tctggatgat aacggttcgt tgctgctgga ttttggccca
     6901 cgccagttca ccgtcagctt tgatgaaacc ttaaaaccgt ttgtgcgtga tgtttccggc
     6961 agccgcctga aagacctgcc aaaaccaaac aaaagcgatg atgagtcaca ggcgaacgat
     7021 gctgttaacc gctacaaatt gctgaaaaaa gatgcgcgta ccgtcgccgc ccagcaggta
     7081 gcaaggcttg aatccgccat gtgcctgcgt cgccgctggt cgccggaaaa cttccaactc
     7141 ttcctggttg tgcatccgct ggttcgccac ttaacccgcc gtctgatttg gggcgtttat
     7201 agcgccgaca accagctact ggcctgtttc cgggtagcgg aagataacag ctacagcacc
     7261 gctgacgatg atcttttcac cctgccggaa ggcgatatct ctatcggcat tcctcacgtt
     7321 ctggaaatat caccgacgga tgctgtcgcc tttggtcagc tttttgccga ctacgaactg
     7381 ctaccaccgt tccgtcagct tgaccgtaac agttacgccc tgacagaagc cgagcgcaac
     7441 gccagcgaac tgacccgctg ggcaggcaga aaatgcccga gcggccgggt c
//
LOCUS       DADLSA010000172         7405 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.173, whole genome shotgun sequence.
ACCESSION   DADLSA010000172 DADLSA010000000
VERSION     DADLSA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.173"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..267
                     /locus_tag="JN691_003598"
     CDS             <1..267
                     /locus_tag="JN691_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7244927.1"
                     /translation="GDVLYPVKITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRL
                     VIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(343..807)
                     /locus_tag="JN691_003599"
     CDS             complement(343..807)
                     /locus_tag="JN691_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY7244928.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAQGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEADYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(804..1679)
                     /locus_tag="JN691_003600"
     CDS             complement(804..1679)
                     /locus_tag="JN691_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAY7244929.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1676..2365)
                     /locus_tag="JN691_003601"
     CDS             complement(1676..2365)
                     /locus_tag="JN691_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAY7244930.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2413..3903)
                     /gene="nanT"
                     /locus_tag="JN691_003602"
     CDS             complement(2413..3903)
                     /gene="nanT"
                     /locus_tag="JN691_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAY7244931.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKDKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4013..4906)
                     /gene="nanA"
                     /locus_tag="JN691_003603"
     CDS             complement(4013..4906)
                     /gene="nanA"
                     /locus_tag="JN691_003603"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAY7244932.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5028..5819)
                     /gene="nanR"
                     /locus_tag="JN691_003604"
     CDS             complement(5028..5819)
                     /gene="nanR"
                     /locus_tag="JN691_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAY7244933.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            6199..>7405
                     /gene="dcuC"
                     /locus_tag="JN691_003605"
     CDS             6199..>7405
                     /gene="dcuC"
                     /locus_tag="JN691_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY7244934.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHF"
BASE COUNT         1819 a         1966 c         1870 g         1750 t
ORIGIN      
        1 ggtgatgtgt tgtatccagt aaaaataacc agaaaatcat caggaaaaac tgccttccac
       61 cttgttccat tcggcttaaa taagactcac gaccttctag aggtggaaga tgcttcagaa
      121 gcaattcggc tggtgattga tgaacgtcat tccattcgct gcagtacact tacagcaact
      181 attacgaata aaaagggcaa gcgaattaag cggacaggta tatacagtat caagggtgtt
      241 aacataaaag aatacaacgt caggtaatgg cacagagcgg tccgtcagtt caggtttggc
      301 tctgtgccaa agatgggtca ggtcacacct gatctaataa atttaagcca ttaaatcagc
      361 cttaaccttc accaccacct ttttaatctc acccggctcg ccaacaacgc atcccggctt
      421 atgcggctct cctggcataa acacggcgaa cattcccggt ttcaggatga tggtttgctc
      481 gttttcaatg gcgctacaaa gctggtaatc agcttcatgg tggaactctt cacattggcg
      541 cgcagtgcct gccatgccaa acagaatccg ttcctcaccg tttaataaca gctggatatc
      601 aatgtattgc tcgtgcaatt ccgctttttt ctcgacgggc gattgagtgt taaacgtcat
      661 gacattcata aaaatattat cgccctgtaa ttcgtaacga ccctgcgctt tttcttgcgg
      721 tctggcagct aaggccagcg ttaacgcgtc ctgtaacgca ggatgtaacc cagcagacgg
      781 taatgactgt acttcaccca tcatcataat ttttctccct gggccaacag cgcagcccca
      841 agtaaacctg catcatggcg gtaatgcgcc gccagtaaat caacatgaaa tgccgctggc
      901 tcctgcgcca gatacatttc caccaacgcc agataccctt ctgccagacc aacgctgcca
      961 ccaaccacca cgcactggca atcagttgtg gctttaatat cagcgatcag ccttgcaagc
     1021 gtacgtgcgg agcgatgaat cagttgctgc gcctgctcat cgccctgccc ggcgcgtgtg
     1081 aaaatagttc tcgcatccgc gccagccagc tcgccctgcg ctgccgccgc aatgccgcga
     1141 ccagaagcaa ttgcttccac gcaacctgtg cgtccacagc cgcagactgg gccgtgtgga
     1201 tcggcaagcg tatgcccgat gtgccccgcc agaccgccag ggccggtaag cagtttgccg
     1261 ccgctcacta caccgccgcc gacgccagtg gaaacggtga taaagaccat atcggttata
     1321 tcgccttcca gcgcctgata ctctgcccat gctgcggcct gcgcgtcgtt aatggcaatg
     1381 gtcggcaaat tggtaagttg ttccagcgtt ttgactaacg gaaagtgtag caatccaccg
     1441 agattatgcg gattcagcgc cagcaaactg ccgtcgcgaa taattccggt tgaagcgatg
     1501 gcaacccgct gcgcatgagc ttgcaacgga gagactaatg cggataaggc atcacgcaag
     1561 gcttctggtg tctggctggc tggcgtagga agttcacgac gatcgcggat ctgcccgtca
     1621 gcgccaatca gcgcggcggc aagtttagta ccgccgatat caatcgccag tatggtcata
     1681 gcaccgcctt tttcatcgct gtgttgtacc actgacaaat gtgttcaaga cgcgtaatgg
     1741 cagaaccgac cgtcaccgcc cacgcgccgt ggcgcatcgc atctgccgcc tgagcaggcg
     1801 tgttgtaacg cccttcggca atcacccgac atccggcttc gctcaacgtt ttcaccagcg
     1861 ccagatccgg ctcttctggc gtttcaggcg tggtatagcc agaaagcgta gtgccaataa
     1921 tttcggctcc cagcttttgg catgccaggc cgtcttccgg cgttgagcag tcggtcatcg
     1981 ccagtaaacc gtgatgatgg atacgtgcca gcagcgtttc aacaggcacc ggacgcgggc
     2041 ggtcggtgcc gtcaatggcg ataatgtccg cgcccgcctg cgccagcgca tcgacatctt
     2101 caatataggc cgtgatgcgt accggagaat cctccagatc gcgtttcaca attccaataa
     2161 tcggcacgct caccaccgca cgcgtggctt gcagatttgc cacaccttcg atgcgaatgg
     2221 caaccgcgcc cgcctgctct gccgctaatg ccatggctgc gacgatttcg ggtttatcga
     2281 gcgggctgtc aggaaccggc tggcaggaga caatcaggcc accgttagca gcgatttttt
     2341 gatccagttg tgcaagtaac gacatacatc ttcccttagc gaaaggcccg gtacatagac
     2401 cgggcaacag gattaacttt tggttttgac taaatcgttt ttggcgctgc caaatggcac
     2461 ggcaccgcta aatggtttgc cgtcgatagc gtcatgagta cgcaatgctt ccgggcgcag
     2521 ccaacgctga acgcgggaag gcatatccag accaatcagc aggatcacca caaatgtcag
     2581 gctgaacgag agcgatgcca gcgcagtacc cagatccaga cgttgagcga tcaacgcgcc
     2641 gatgattggg gccagtgcgc cacccaatgc gccaacgttg taggtaaagc ccaggcccgc
     2701 tgcacgctgg tcggtatcga aataaccgcc aatcagtttc ggtaagatcc cggcgatccc
     2761 ttgtccaagc atttgctgga agaacagtaa cagaccgagc acccagacgt ttgcgccgcc
     2821 aatcgcaaat accggaataa tcagcagctg cgaggccagc aggctacaaa cgtacgcttt
     2881 gcgggttccc agccagtcac cgaggaagcc acctacgcag catcccacgg ccgcgccaaa
     2941 gccactaaag aacagcacat tggcgacagt atgcgggtta taagccagat cggttttcag
     3001 atacgttggc agcagcgcct gaatcggcca tgagtagagg aaagcaaaca acacgaccac
     3061 catcagcatt acgcccgttg gccagcgttt acccgtactt tgcaccataa agctgataaa
     3121 gattgcggcg cataacagcc cgagaacagc gacgattgca gcattttgca ggttaccggc
     3181 gaagcagaac cacagcgcag tagccgccgc cagtgtcatt acgatattgg caatgcgatg
     3241 ttcaccacgg tagagaatat ccaccattgt gcgtactggt gctttacctg cgtgtttatc
     3301 tttccagtct tccgcttccg ggatgttttt acgcagccag agagcaaaga tgattggcaa
     3361 aatgccgata aagaacagcg cacgccaacc ccagaccgga accaccaggc tatagacctg
     3421 agcggcaacg acggccccca cagagaagcc tgaaatcaaa aaaccactgg ctttgttacg
     3481 cagatgtttt ggccagcttt caatgacata ggtggcgctg gaaccgtatt cacccgccat
     3541 ccccatgccg atgaccagac gagcgataaa catggtgatg tagcctggcg caaagccgca
     3601 ggccagcgtc ccggccgaga agagaacgat gctggtgacc attgccagac gacgcccgta
     3661 gcggtcaccc atagcgccga gcatcaggcc gccgaaccag cgagagataa aggctgcgga
     3721 gatcagactt gccgcctgca ccgtcgtcag cccgaattca ccctgtactt cggtgagtac
     3781 cagggcgatt aaaacgaaat caaaaccgtc aagcagatat cccaaccagg cagcggaaaa
     3841 tgcgcgccat tgtgcacggt tgagatggcg ataccacggg atattctggg ttgtagtact
     3901 cattgtgagt ctcccgcggt gggcgatgcc cacacgcttt ggtatgaaaa ttgtagggta
     3961 cagatgcgtt tatttcccct cacccggtag gggcgagcga ggggaaaaca actcacccgc
     4021 gctcttgcat caactgctgg gccagcgcct tcagttctgg cagatatttt tcatctaccg
     4081 gtccaaacgg tttgcggcac agcggcacag aaacgacatc catataatgc aggacagttt
     4141 tcaggccgcg gaatacgccc gttttgatca gtaaatcaat gactttgttg cactcagttt
     4201 gcagtttctg cgcggtctgg atatcgcctt ctttcagcgc cttcacgatc ccctgatatc
     4261 gccagcccat gatgttgtaa gtactgccga taccaccatc agcgcctgcc agcaagccgg
     4321 aagcgaagat ttcgtcgtaa ccgttataga gcaccagatc aggatgttca cgacggatct
     4381 gttccatctg atagagatcg ccagaagtct gtttcagcgc accgacgcca ggcaacgtaa
     4441 ccagggtgtt gatctgatcc agggtcagtt ttaccccact cagggctgga atgttgtaca
     4501 ccaccatcgg caaaccatcc gctgaatcaa taattgcccg atagtgatcg cagtgttctt
     4561 caaagctgaa aggatagtag aacggcgtga cggcagagac ggcatcgaag ccataacgtt
     4621 tagccgatgc cgcaagttgt tggctttcgg cggtgctgac gcaaccgacg tgggcgatga
     4681 gtttaatctt acctttcgcc tcttcggcga cgatttccag tacctgttca cgctcggaaa
     4741 ggctttgtac aaaggcttcg ccggtcgaac cacccacgta taaaccgtcg atgccctgct
     4801 gaatattgaa ctgaaccagg cggcgcagac tcgctttatc cagtgcttgt tgttgatcaa
     4861 aaggagtcag gagtgcagcc attacgccac gtaaattcgt tgccataaat acctctgaag
     4921 tgatgcttgt ctgataaacg atataccttt atacctgtta taccagatca attaagcaac
     4981 accccataca gaaagcttat aatgcgatct gcttcactaa agtggcatta tttctttttg
     5041 ttggtggtct gaccgaaagc gtgccaggta gcagagacgc tgttgagatg cgattgcaac
     5101 gcacgatcgg cttcgtcagg atcatgacgg cggatcgcat caacgatcgc aatatgctgt
     5161 tgataactaa cgttgttatg ttcgtgcagt gcctgatcgg taaccgttgg gcgtgcggca
     5221 ataagccagt cgagcagggc aacgtggatc gccatgaaga ttgggttacc ggggatctcc
     5281 gccagcacgc ggtggaaatc aacgtctgaa cgaatgaatg ccgcgttgtt atccagcgac
     5341 tgactgttga tttccagtgc ttttgccagc aaatcgattt gctcatcggt ggcgtgttca
     5401 gccgcatagc gcaccaggct ggattcaaag aacagacgta attgttcgaa atgggcaatc
     5461 ccaccaggat gagaaaggaa atctttcgcc atgccggaaa gctcgccgat gatggtgtcc
     5521 gcagaaggtc gagagacacg agcgcgttcg ccgttgttta tttgcaccag acctttgcgt
     5581 tttaacgctg ccagcgcttc acgcacagaa ggacgcccga cgttaaagaa cgccatcagt
     5641 tcgcgttcag acggtaattg ttcaccttcg ccaaattcac gacggcggat catctgttcc
     5701 agctcttctt ccaccatttc ggagagtttt ttacgcgcca gcgggcggct acgcaagttg
     5761 cgaccaattg caggtgaaga atcttcggtt tgcgaatcaa atgcgttcat aaggcccatt
     5821 ctgtaaggtc agtgtgatta acatcatcag tgacatccta tcacaggatt gaaagtaggg
     5881 gaaaatggca ggtttttctc tttgtgcctc atcattacca taattaacgg aataattaac
     5941 tattgcgaaa aattaatgta acgcagataa acacatcccg attgaattat tcataagact
     6001 attcacaagc attatgaata ttatgaatgt gttcttacaa aataatcata atcgcatatt
     6061 ttttaatgaa aaatcacctc acctacaatt aaaaacacga tatccgcacc ataaatagac
     6121 ttgcaaaaaa tataacatca ttgttttcaa tctgccgttt atgggattga ccgttttctt
     6181 ttgacacgga gttcaacaat gttcggcata attatatctg tcatcgtatt aattacgatg
     6241 ggctatttga tcctgaaaaa ctacaaacct caggtagtgc tggctgccgc aggtgtcttc
     6301 ctgatgatgt gcggagtctg gttagggttc ggtggtgtac tcgatcccgc caaaagcagc
     6361 ggctacttga tcgtcgacat ttataatgaa atcctgcgca tgctgtccaa ccgcattgcc
     6421 ggattggggc tgtcgattat ggcggtgggc ggttatgccc gctacatgga gcgcatagga
     6481 gccagtcgcg cgatggtgag cttgctaagc cgcccgttaa aactcattcg ctcgccgtat
     6541 attattctgt cggcaactta cgtcatcggc caaatcatgg cgcagtttat taccagcgcc
     6601 tccggtctgg gtatgttgct gatggtcacc ttatttccga cgctggtgag tctgggagta
     6661 agtcgcctct ctgcggtggc ggttatcgca accacgatgt ccattgagtg ggggattctg
     6721 gaaacgaact ccatttttgc agcccaggtc gcgggaatga aaattgccac atacttcttc
     6781 cactaccagc ttccggtcgc ctcttgcgtc attatctcgg tggcgatctc ccactttttc
     6841 gtgcaacgcg cttttgacaa aaaagataaa aatatcaatc acgaacaggc agagcaaaaa
     6901 actctcgata atgtcccgcc gctctattac gccattttac ctgtgatgcc gttaatcctg
     6961 atgctcggct cgctgttcct cgcccacgtc gggctgatga agtcagaact gcatctggtg
     7021 gtggtgatgt tactaagttt gacggtgacg atgtttgttg agttcttccg caagcataac
     7081 ttgcgcgaaa caatggacga tgtgcaggcg ttttttgacg gcatgggtac gcagtttgtc
     7141 aacgtggtaa cgctggtggt cgcgggtgaa atatttgcga aaggcttaac gacgattggc
     7201 actgttgatg cggttatcag gggtgcggag cattctggtc tgggcggtat tggcgtgatg
     7261 attattatgg cgctagtcat tgccatttgt gccattgtga tgggctctgg caatgcgccg
     7321 tttatgtcat ttgccagtct tattccgaat atcgcagccg gactacatgt accagcggtt
     7381 gtaatgatta tgccgatgca ttttg
//
LOCUS       DADLSA010000173         7311 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.174, whole genome shotgun sequence.
ACCESSION   DADLSA010000173 DADLSA010000000
VERSION     DADLSA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.174"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..457
                     /locus_tag="JN691_003606"
     CDS             <1..457
                     /locus_tag="JN691_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7244935.1"
                     /translation="FLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAF
                     PFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVAS
                     VVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRS"
     gene            559..2013
                     /gene="amn"
                     /locus_tag="JN691_003607"
     CDS             559..2013
                     /gene="amn"
                     /locus_tag="JN691_003607"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY7244936.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2356..3072
                     /locus_tag="JN691_003608"
     CDS             2356..3072
                     /locus_tag="JN691_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7244937.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3528..3603)
                     /locus_tag="JN691_003609"
     tRNA            complement(3528..3603)
                     /locus_tag="JN691_003609"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3568..3570),aa:Asn,seq:gtt)
     gene            complement(3704..5347)
                     /gene="yeeO"
                     /locus_tag="JN691_003610"
     CDS             complement(3704..5347)
                     /gene="yeeO"
                     /locus_tag="JN691_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HAY7244938.1"
                     /translation="MLRYILTAKNILSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSSFEMWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVQGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            5352..5427
                     /locus_tag="JN691_003611"
     tRNA            5352..5427
                     /locus_tag="JN691_003611"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5385..5387,aa:Asn,seq:gtt)
     gene            complement(5465..6415)
                     /gene="cbl"
                     /locus_tag="JN691_003612"
     CDS             complement(5465..6415)
                     /gene="cbl"
                     /locus_tag="JN691_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HAY7244939.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFARKGLPV
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6517..>7311)
                     /gene="nac"
                     /locus_tag="JN691_003613"
     CDS             complement(6517..>7311)
                     /gene="nac"
                     /locus_tag="JN691_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HAY7244940.1"
                     /translation="ELNQQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNV
                     GQSLSGQVSIGFAPGTAASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLD
                     MAVIYEHSPVAGVSSQALLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLR
                     VDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITT
                     PSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQWQLVS"
BASE COUNT         2059 a         1756 c         1588 g         1908 t
ORIGIN      
        1 tttccttaat attggtttgc tggtaggtgg attaagctgc ctgacaattc cctgttttgc
       61 ctggcttgcc gatcgttttg gtcggcgcag ggtttatatc acaggcgcgt tgatcggaac
      121 gttgagcgca tttcctttct ttatggcgct tgaagcacaa tctattttct ggatagtttt
      181 cttctccata atgctggcaa acattgcgca tgacatggtg gtgtgtgtgc aacaaccgat
      241 gtttaccgaa atgtttggtg ccagttatcg ctacagtggt gctggagtcg gttatcaggt
      301 tgccagtgtg gttggcggtg gatttacacc ttttattgcc gctgcactca tcacttactt
      361 tgccgggaac tggcatagcg tcgccattta tttgctggct ggatgcctga tttctgcaat
      421 gaccgcttta ttgatgaaag acaatcaacg ctcgtgatag cctggcgaag atcatccgat
      481 cttcgcctta tacttttgtt tcacattcct gtgacatact atcggatgtg cggtaattgt
      541 atggaacagg agacacacat gaataataag ggctccggtc tgaccccagc tcaggcactg
      601 gataaactcg acgcgctgta tgagcaatct gtagttgctt tacgcaacgc cattggcaaa
      661 tatattacaa ttggcgaatt acctgatgaa aacgcccgta aacaaggtct ttttgtctat
      721 ccatcactga ccgtaacctg ggacggtagc acaacaaatc cccccaaaac gcgcgcattt
      781 ggtcgtttta ctcacgcagg cagctacacc accacgatta ctcgccctac tctctttcgt
      841 tcgtatctga atgagcaact aacgttgctt tatcaggatt atggtgcgca tatctctgtg
      901 caaccctcgc aacatgaaat cccttatcct tatgtcatcg atggctctga attgacactt
      961 gatcgctcaa tgagcgctga tttaactcgc tacttcccga caacagaact ggcgcaaatc
     1021 ggcgatgaaa ctgcagacgg catttatcat ccaacggaat tctccccgct atcgcatttt
     1081 gatgcgcgcc gcgtcgattt ttccctcgca cggttgcgcc attataccgg tacgccagta
     1141 gaacattttc aaccgttcgt cttgtttacc aactacacac gttatgtgga tgaattcgtt
     1201 cgttggggat gcagccagat cctcgatcct gatagtccct acattgccct ttcttgtgct
     1261 ggcgggaact ggatcactgc cgaaaccgaa gcgccagaag aagccatttc cgaccttgca
     1321 tggaaaaaac accagatgcc agcatggcat ttaattaccg ctgatggtca gggtattact
     1381 ctggtgaata ttggcgtggg accgtccaat gctaaaacca tctgcgatca tctggcagtg
     1441 ctacgcccgg atgtctggtt gatgattggt cactgtggcg gattacgtga aagccaggcc
     1501 attggagatt atgtccttgc acacgcttat ttacgcgatg accacgttct tgatgcggtt
     1561 ctgccgcccg atatacctat tccgagcatt gctgaagtgc aacgtgcgct ttatgacgct
     1621 accaagttag tcagcggcag gcctggtgag gaagtcaaac agcggctgcg tacaggtact
     1681 gtggtaacca cagatgacag gaactgggag ttacgttact cagcttctgc acttcgtttt
     1741 aacttaagcc gggccgtagc aattgatatg gaaagtgcaa ccattgccgc gcaaggatat
     1801 cgtttccgcg tgccatacgg gacactactg tgtgtttcag ataaaccgtt acatggtgag
     1861 attaagcttc ccggccaggc taaccgtttt tatgaaggcg ctatttccga acacctgcaa
     1921 attggcattc gggcgatcga tttgctgcgc gcagaaggcg accgactgca ttcacgtaaa
     1981 ttacgaacct ttaatgagcc gccgttccga taataagtta tgaaccttct tatcagaaat
     2041 agatgtgaag gattaagtaa gacatctggc aaatagcctg caataaacgc gggctgcttg
     2101 cttccttggg cagatgcgag ttttattatc gtcttaatga tttccacata ttaaaagcaa
     2161 gtatgctttc aaaacacaat tataaaaaat cccgccaaca acattagttt ttataaaatt
     2221 aaatataaga ttatggcttt agaatatttt tatttctaat agacgagatt tttcctgtta
     2281 tgatataata tgccgaatta acacatgtta acgatttacc agtaatgtaa ataaattttc
     2341 gaggagatca ttccagtggg acgtaaatgg gccaatattg ttgctaaaaa aacggctaaa
     2401 gacggtgcaa cgtctaaaat ttatgcaaaa ttcggtgtag aaatctatgc tgctgccaaa
     2461 caaggtgaac ccgatccaga attaaacaca tctttaaaat tcgttattga acgtgcaaag
     2521 caggcacaag ttccaaagca cgttattgat aaagcaattg ataaagccaa aggcggcgga
     2581 gatgaaacgt tcgtacaggg acgttatgaa ggctttggtc ctaatggctc aatgattatt
     2641 gctgagacat tgacttcaaa tgttaaccgt acgattgcta acgttcgcac aattttcaat
     2701 aaaaaaggcg gcaatatcgg agcggcaggt tctgtcagct atatgtttga caatacgggt
     2761 gtgattgtat ttaaaggaac agaccctgac catatttttg aaattttgct tgaagctgaa
     2821 gttgatgttc gtgatgtaac tgaagaagaa ggtaatattg ttatttatac tgaacctact
     2881 gaccttcata aaggaatcgc ggcgctaaaa gcagctggaa tcactgagtt ctcaacaaca
     2941 gaattagaaa tgattgctca atctgaagtt gagctttccc cagaagattt agaaatcttt
     3001 gaagggcttg ttgatgccct tgaagatgac gacgatgtac aaaaagttta tcataacgtc
     3061 gcaaatctct aattatattt taaagaaatc tgtctttatg gcagatttct ttaatctcat
     3121 ataattctta taaaaaatat aatgtttaac tcgtcatatt gcttataaaa aatccccgtt
     3181 cccagagaaa taatatttat taaaatggca ggtcttcttt ttctgattac cgaagacaaa
     3241 gtggcaatta cgtatagttt cccgataaag acgcgacagc tacatcccgc ataaggcatt
     3301 tttctcttta tctttgtacg gtacttcatg gaacagaatt tttgaccttg tgaatcgtga
     3361 tgtctgttgg ggaggggcaa tttgctcact gaagcgtgag actcgattaa gcgcacgaaa
     3421 cacagaaatc aaaaaacccg gccacttttt tacaaagtgg ccgggtaaaa ataattttta
     3481 ttttttaact gtcctgagtc tcatagagat gtctcaaaac taaaatttgg ctcctctgac
     3541 tggactcgaa ccagtgacat acggattaac agtccgccgt tctaccgact gaactacaga
     3601 ggaatcgtga gaacgaggcg aatattagcg atgcccaccc acaatgtcaa agcctgtttt
     3661 ttaaatttga aatcgtttgc tgaaataatc tgcattttgt cgtttattcc gacacaactg
     3721 gctttttttc acacttttgc ggctcggatc gagggtattt ccacagccaa cgtccagtaa
     3781 ccattcgcca gtaaaacagc acggcccgca cagcccagtc ggcaaacatt cccatccaga
     3841 caccaaccac accccagcca agcatgattc cctgcacata accgactaca acccggcaac
     3901 cccacatgct caacatagaa acccacatgg cgtaacgggc atcacgagca cccttaaatc
     3961 cagcgggtag cacccatgag gcggaccaaa taggcataaa taaagcattt agccaaatca
     4021 gaatcacaac gacgtgttta acctgtggat cctgggtgta aaacgatgcc ataaccccgg
     4081 caaagggagc cgttagccag gcgatggccg ttaatccaag agtggacagc cagaacacat
     4141 gccgcaactg aatctctgct tgcgctatct gccctacccc caaccttcgg cctgtaatga
     4201 tcgtagaagc agaaccgagc gcacttccgg gtaagttgat aagagccgca attgagaacg
     4261 cgataaaatt tccggcaata acgctggtcc ccatcccggc aacgaacatt tgggttaata
     4321 accgaccact ggtaaataac actgattcga cactcgctgg aataccaatc cccatgactt
     4381 cccagataat gctaaaattc agcggtttaa aatagctctt taacgaaatc cttagcgcag
     4441 gattaaaacc aatcgccagc acccacaaaa ttgcaactgc gccaatataa cgagaaatgg
     4501 ttaaacccaa cccagccccg acaaatccca gtcccggcca ggagaaaagg ccgtaaatca
     4561 atatgccgct aataataata ttaagaatat tcaggctacc gttaatcagt agcggtattt
     4621 tcgtattccc tgcaccacga agtgccccgc taccaataag agtgatggct gcggctggat
     4681 aactgagtac ggtcagctcc agataagtca acgccagtgc tttaacttct gtcgtggcat
     4741 cacccgcgac gaaatcaata atttgttcgc caaaatgatg aattagcgtt gccaacagta
     4801 cggcaaacaa cgtcatgatc accaatgact gccgcgtcgc caccctcgct cgtcgtcgat
     4861 cccgcttacc gagactaaat gctacaacga cagtagtacc aagatcgata gctgcaaaaa
     4921 aagccataat gaccatattg aagctgtccg ccaaccccac gccggccatc gcatcttttc
     4981 ccagccagct gaccagaaaa gtgctcagga cccccatcaa caggacacag gcgttctcca
     5041 tgaagatagg aacagcaagc ggggttatct cgcgccagaa caacactttg tagctcttgc
     5101 gtttagagtg ccagcgagtg ccgtgaacaa cctggcgtaa agcagaggag atattcaaag
     5161 ccgaccttaa ttgcagaaag tgaaaccaca tttcaaatga tgagggagaa tcagcaaagc
     5221 tgcaaagatt ttcgccaaca aattgtctgc aaatgcaaca aactgttgat agaaacggca
     5281 aacagttggg aaatttaaaa atcgggtttg acaaaatatt tttcgccgtt aagatgtacc
     5341 tcaacagcga ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca
     5401 ctggttcgag tccagtcaga ggagccaaat ttctgaaaag cccgctttta tagcgggctt
     5461 tttgctatat ctgataatca atttcctctt cactgttttc catcacctgg cgcttgatat
     5521 cctcaactga cagtcctgca ttacaaagtt ccagaaagcg ccaaacatag ttacgctgaa
     5581 gttgtcctcg cttcagtccc aaccagacag tattagcatc gaaaagatgc cgcgtatcca
     5641 ggcggattaa attctcttcc tcttgttcgc cgctggattg ttcggcaact aatccgatcc
     5701 caagcccaag agcaacatag gttttaatga catcagaatc ctgtgcactt aatacaatat
     5761 ctaccggcaa acctttgcgg gcaaatgcgt catcagtgcg tgagcgcccc gtaattccct
     5821 gtcggtaagt aattaacggc cacttcgcta ttgattccag cgtcaatggt gtaatttgcg
     5881 ttaagggatg atcgagtgga acaagcaaac tatggtgcca acaaaaccac gggaaggcga
     5941 cgagctgcgg gtcattactc aaacgctcgc tggcgatacc aatatcagct tcgccatttt
     6001 gcaacaacgt cgcaatttcc tgtggcgaac cctggattag ctcgagccga acctccggga
     6061 aaagttcgcg aaaagcttta atgacctctg gcaagctata acgtgcctga gtatgcgtcg
     6121 ttgcaatagt gagaacacca gacgtatcgt tggtaaacag gtctgcaagc cgacgaacat
     6181 tactggcttc attcagaata cgttctgcaa tgaccagtaa tgctttgccc ggttcagtca
     6241 tgcccagcag tcgcttacct cgtcgaacaa atatttcgat gccaagttca tcctccagtt
     6301 cccgaatatg acggctaacg cctgactgtg aggtaaaaag catattcgca acctctgtca
     6361 ggttgtaatc ctgacgtgca gcctcgcgga ttatctttag ttgttggaaa ttcacggtaa
     6421 actccgggca gttcagatat cccgttattg ttaaagtcta atgcccggca taacaaataa
     6481 taaaaacccg catcttattc catcccgata taacgcttag ctcaccaatt gccactgcct
     6541 tttttccatc actggagaac taatcactga cattaacaac tctttcactg cctgtgcctg
     6601 tggtgacaag tttgctctgg cgggtaaatt taaggacaaa gagagactca ttgaaggagt
     6661 ggtaatgcgt gacatccacc catttactgc gccacataac gaacgcgcag ccgattcagg
     6721 taatactgca acgcccatgc cgctggcaat cgctgcggta agcgtggcaa tagactcaat
     6781 ttcaccaatg acttttgccg tgagccgccg tagggaaaaa gcctcatcaa cacgaagtct
     6841 aacagcgctg taatcactgg ggagaaaaag gttcatttgc gcaatagcat taacatcaac
     6901 gttttgcccc gggcaatctt gagttcctac caggaaaaga tcttctttca gcaaagcctg
     6961 actggatacc ccagccacag gggaatgctc ataaatcacc gccatatcga gttggtgatt
     7021 tatcagtttt tcgttaagca ctgcaccact attttcatga agatagataa cgacctccgg
     7081 gaattcagcg cgaaccgcct gtaataaggg catggtgatg gatgacgcag cggttcctgg
     7141 cgcaaagcca atcgagactt gccccgataa tgactgacca acgttatgca ccgccagttg
     7201 ggcctgctca cactgacgta aaatggcccg tgcatgggta tagagaattt ttccggcgtc
     7261 tgttggcgta acgccccgtt ttgtgcgaat caaaagctgt tgatttaact c
//
LOCUS       DADLSA010000174         7252 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.175, whole genome shotgun sequence.
ACCESSION   DADLSA010000174 DADLSA010000000
VERSION     DADLSA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.175"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..186)
                     /locus_tag="JN691_003614"
     CDS             complement(<1..186)
                     /locus_tag="JN691_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244941.1"
                     /translation="MLQSSADKACNVLVKKVRYIKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAF"
     gene            264..1358
                     /locus_tag="JN691_003615"
     CDS             264..1358
                     /locus_tag="JN691_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HAY7244942.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1382..1594)
                     /locus_tag="JN691_003616"
     CDS             complement(1382..1594)
                     /locus_tag="JN691_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 family protein"
                     /protein_id="HAY7244943.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1854..2162
                     /locus_tag="JN691_003617"
     CDS             1854..2162
                     /locus_tag="JN691_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAY7244944.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2221..3315)
                     /gene="yaiW"
                     /locus_tag="JN691_003618"
     CDS             complement(2221..3315)
                     /gene="yaiW"
                     /locus_tag="JN691_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAY7244945.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPSLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3328..4548)
                     /gene="sbmA"
                     /locus_tag="JN691_003619"
     CDS             complement(3328..4548)
                     /gene="sbmA"
                     /locus_tag="JN691_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAY7244946.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4900..6057
                     /locus_tag="JN691_003620"
     CDS             4900..6057
                     /locus_tag="JN691_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-12 family class D beta-lactamase AmpH"
                     /protein_id="HAY7244947.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6058..6561)
                     /locus_tag="JN691_003621"
     CDS             complement(6058..6561)
                     /locus_tag="JN691_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7244948.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(6649..7176)
                     /locus_tag="JN691_003622"
                     /pseudo
     CDS             complement(6649..7176)
                     /locus_tag="JN691_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1841 a         1849 c         1747 g         1815 t
ORIGIN      
        1 aaaagctatg aaatatattc tcttcgttaa cttataatta agagaaaaaa actcaccgca
       61 ttgccattta agccaggaat aaattagctt cccgttcatc atttttgtga tcttgcgcac
      121 atctttgata taacgcactt tcttcaccag tacgttgcac gctttgtcag cagaagattg
      181 caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg tggtaatgta tgcactttgt
      241 gttagacggg ataggttttt aagatggaaa aactgcgggt aggaatcgtt tttggtggta
      301 aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa cattgtcgat gccattgata
      361 aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca agggcaatgg cacgtcagcg
      421 atgccagcaa ttatctgcta aatgcagacg atcctgccca tattgcgttg cgcccttcgg
      481 cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca gcttatcgac gcgcaaaacg
      541 gtcagccgtt gccgacggtg gatgtcattt tcccgattgt ccacggtacg ctgggcgaag
      601 atggttcctt gcagggaatg ctgcgggtcg ccaatttacc gtttgtaggt tctgatgttc
      661 tggcttcagc agcctgtatg gataaagatg tcaccaaacg tctgctacgc gatgccgggc
      721 tgaacattgc gccatttatt accctgacgc gcgctaatcg tcacaacatc agttttgccg
      781 aagtggagtc taaactgggg ttaccactgt ttgtaaaacc ggctaatcag ggctcttctg
      841 ttggtgtcag caaagtaacc agtgaagaac agtacgcaat tgccgtcgat ctggcgttcg
      901 agttcgatca taaagtgatc gttgagcaag ggatcaaagg tcgtgagatc gaatgcgcag
      961 ttctgggcaa cgacaatccg caagccagca cctgtggcga gatcgtactc accagcgatt
     1021 tctatgccta cgacaccaag tacattgacg aagatggcgc gaaagtggta gttccggcag
     1081 ccattgcacc agagatcaac gataagatcc gggcgattgc cgttcaggct tatcagacgt
     1141 tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac cccagagaac gaagtggtga
     1201 tcaacgagat caacacactg cctggcttca ccaacatcag tatgtatccg aagctgtggc
     1261 aagccagcgg tctgggttac accgatctga tcacacgttt gattgagctg gcgctggagc
     1321 gtcacgctgc ggataacgca ttgaaaacca caatgtaata ttttaacgcc cgataatatt
     1381 cttattgggc gttttcagtt tcttcatcgc gacgacgaat aatcaaccct gccagccaga
     1441 aactaatcac ccacgtcacc agaccgacgg cataggtctg ccaacctttt gcttcaaatc
     1501 ccagtacccc caccacgcca ttaagaatga atataaggcc aatggcgaac gcatagtagt
     1561 gccagtcacg gcggattttt acaggcagat tcatggcagc tccgttaacg aaaaacaaca
     1621 tccttgcata ttctgtaaca accgtctgtg gtgtagacaa cattttcgta attgttaata
     1681 aatttaactt aaggttgcca ttttgtgact cacaacgaag attcacgctt cgggaatatt
     1741 cccaacatca atttaccatg attctgatat tgacaatcta aatgacctgt cagacttgcc
     1801 ataagccgct ggtatcacac cagaagagaa gcagatgact tccggaggtt tttatggctg
     1861 atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg caatgcctct tcaacgcctg
     1921 gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg ggctggggcg caattgctga
     1981 acctgttagt gcatgcgccc ggcgtctatg agcgtttaat gcaggtccag gaaacaggtc
     2041 gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt cgggctgatc ccgtttttag
     2101 caggctgcct catttttgca gtggtggcgc tatggctgca ctggcgacat cgccgccagt
     2161 aaaatttacg ttgccagatg cggcctgaaa cgccttatct ggcctgacat tcctgtttga
     2221 tcactgtttc atacaacgcg cccgccgttc gtctacgcgc ttcgcaaacc aggccgtagt
     2281 caggttgcgg gtgattttcg ggctttccag ttgaatgcca ggcaacattt cgcggggtag
     2341 tgatttaccc gttttcgctt cggcaagttg ataaactttc ttgtatagcg ccgtctcctc
     2401 aaaagagaag ctgtcacctt tctctaactg acggcgaatt tcgctgtcgc tcatccccag
     2461 ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg ggttctttac tgtcgtaacg
     2521 aattaaatca ccgtccagtg ccagcttcac gccgctggcc ttactgactg cgttctgaaa
     2581 cgcggcatta cggctggcgt accagccagc gttaaaatca gcaaagcggt atatcggtgc
     2641 gctatagctg gcgggataat tcagtaaatg gtaagtacca aaccacaacc cgccgcggcg
     2701 actgaagact tcctgacgaa ctgtaccgtc cattttccac ggataccctt tggtatgctg
     2761 ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg cgcaccggat taagcgaacc
     2821 aaacagcgtc tgccccattg gtaccatgct gatcagatcg tcaaaaatcg cgctcaattg
     2881 cttttctgta cgcaccgaat ctaaccgttc gctataactt ttgccgtttg gcgatttgat
     2941 ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc cgttcagcac gacggtcaat
     3001 ctcttgccag gcgatcttgc ttaagctcgg aacggccgga tctgcctgat aatttgactc
     3061 ctgctgcgcc accgccagca ccgagcagac gttttccagc gtcggtgcca gcccctgact
     3121 ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg tctttcacgc tcgcaggcat
     3181 cttctgccgc acgacgctcg ctacatctat cgctttttcg ccctttttca acggttgcgg
     3241 agcctgacta ctacaccccg ctaaaaccag aaccgccaac aatgacagcg aagatagatg
     3301 attgacgcgc gacattactt ctccttttta gctcaaggta tgggttactt cctgaatttt
     3361 gtcaccatcc agctcatgtt caaagctgcg cagacgtttg tagatagaca tcaactcaac
     3421 cagtgtggtc catgagttaa tcaggtactg gaaagcaccg cgaacctgac caaaaacgtt
     3481 ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca acaattgacg gaaacagcaa
     3541 gaacaaaccg aaaacgttat cgacctgcaa atagagaatg cgggcgatgt tgaaatacat
     3601 atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg ctaaacagct cgcgtaccgt
     3661 aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc agctctttac ggtaggcggc
     3721 ctctactcgc tggtttttaa actccagccc cggcagtttg atccctacca ctgccagcaa
     3781 tccggtcccc atcagcgacc agacgatagc ggcaatcacc agaccatacg gaatgtgccc
     3841 gataatcggc agctccggca catgcgcgga gagcgttacc agcaccggca ggaaggcgat
     3901 caacgtcatg atggcgttga taaaactgac gcccatattc tccagcgttg aagcaaaacg
     3961 catggtgtct tcctgcacac gctgtgcggc accttcgata tgacgcagtt gttgccagtt
     4021 cgccatgtaa tattcgttca tcgccgtacg ccagcggaac acgtagtgac tgacaaagaa
     4081 gttgttcagt acactgatca ccaccgcgat cagcgcaatc cccagaaaga cgcccacttc
     4141 gcggtaaaat tgttcgatag tgactttatg cggcgaactt agcgcggttt gaatcagatc
     4201 atagaacggc gcataccagg cgttgacggc gaccccgact tccaccaaaa accaagtgac
     4261 gaagataatc agcgcagtac cgagtattga ccagtattgc caacggtgcg ggctgtagat
     4321 aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag taagcgtaaa aaatcaggaa
     4381 atccaacgac cagaaacgcg cggcgctaat cgggatctgc ccggaagcgc cggtgatacg
     4441 cgccacccag tcacccccac cggcttgcca gaagataacg gcaatcaatg cccaaacaaa
     4501 ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac ttaaacatat gcactcctgc
     4561 taacttctta tcgttttaac aactgctgtg tattaaccgc atgaccgcta caaagcgtca
     4621 gccaaatgaa accgcccatt gtgacccata gcaaggcgct tagttcgcca gcctgaccat
     4681 aagaattgtt tcgacggatt tcacaaacca aagattgttt acataagcgg attaacacgc
     4741 gcaggatcac aaatgcaggc attggtgagc aggaaaaaga tcgtgtttac gcgccgttac
     4801 gtttttaaca ctattattgc actaattcgc cctttgcaat ccatcaatga gtagtataaa
     4861 tacgctcagt taccttcatt caatctatgg acaccaccgt tgaaacgtag tctgcttttt
     4921 tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg cggcgcagcc gatcactgaa
     4981 ccggagtttg cctctgatat tgtcgatcgt tatgccgatc atatttttta cggcagcggt
     5041 gccacgggga tggcgctggt tgttatcgac ggtaaccagc gcgtctttcg tagttatggc
     5101 gaaacgcgac ctggtaataa cgttcgcccg cagctggatt cggtcgtgcg tattgcttcc
     5161 ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc tcgaccaggg gaccgtaaag
     5221 ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac gcgtgccgac ctacaatggg
     5281 acgcccatta ccctagtgaa tctggcaacc cataccagcg ccctgccccg tgaacagccc
     5341 ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc gcgagcaacg ctggaaatac
     5401 ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg cggcgtactc taaccttgcg
     5461 ttcgacctgc tggcagatgc actggcgaat gcgtccggca agccttatac ccagttgttt
     5521 gaagagcaga ttacccgtcc attggggatg aaagacacca cctacacccc ctcaccggat
     5581 cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc cgtgcaataa cacgctggcg
     5641 gcaattggca gcggcggcgt ctattccacg cctggcgata tgatgcgctg gatgcagcag
     5701 tatttgtcat ctgattttta tcagcgcagt aaccaggcag atcgcatgca gacgttgatt
     5761 tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg tccccggcaa agcggatgcg
     5821 cttggtttag gctgggtata tatggccccg aaagagggtc gtccggggat tattcagaag
     5881 acaggcggtg gtggcggttt cattacctat atggcgatga tcccgcagaa gaatattggc
     5941 gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta aaaatatgag cgatggcatc
     6001 aatgatctgg tgaccgagct aagcgggaat aaaccgttgg ttatccccgc atcctgatta
     6061 atactctgaa ggcaaacggt tgatagcgac cagtttgcct ttattcattt cgatatagcc
     6121 gcctttacgc aaagcagcga gaacttctgc gacgacagaa cgcgatatgc gtgtacgttg
     6181 atggatataa ttcatcacac caatacgtga tcgcaattct tcattccagt caatcattga
     6241 taataatgtt gcgcgggttt gtttggctgg tagatgatat cccgtacaat ttccttctga
     6301 tattaatttg tatggtatat cgtttttcat taaaccatca gccagcccca taatataagg
     6361 agcctgggta ataccgataa gtacgttttc ttctctacgc agagagataa ctccttctaa
     6421 aataacgaaa gtatcttcgt tattgacatc actggaaaat ataacttgct tttcattatt
     6481 aaactcgaag cgcgtaccgt atctggacaa acatttatcg agcttaccaa attcctgaag
     6541 aggtttaact acagataaca tttgcgcgtc ctttgcagta atgcccgtca aatccttgac
     6601 gggcattatt tagattaaat taccagtatt tcttcggagt gaagaatatt accaggtata
     6661 tttaacaccc acgttcgcgg accagtcttg atctacgtca ccaccaccga ggtagttagc
     6721 atcggtatag gcgctgaagt tcttggtgaa gctgaactga gtacccagac caacacgtac
     6781 cgcagaccct tcagtaccgt tatcgatgga atcaccgttc acatcgttat cgttgttaga
     6841 gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga gcctgatctt cgctgtaggt
     6901 gaaggtataa cctgcatcta cacccagttc ataacgcatg ctgtcgtaag actgaccgtc
     6961 aactttcatg tcgttgctca gctggtagtc atcaccagac tggaacagac cagaaatgct
     7021 gccgtaagga gtcacgtaac cagcatcacc cagtttgaag tcgtaaccgg ctttcaaacc
     7081 gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa gtaccgttgc tcatggttgc
     7141 agacaggtcg ttgttgaagt gagagtagct caagctatga atcgccacgg ataatctaga
     7201 cacttccgag ccgttgataa tactggtttt catattctgt cggtgacatc tg
//
LOCUS       DADLSA010000175         7159 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.176, whole genome shotgun sequence.
ACCESSION   DADLSA010000175 DADLSA010000000
VERSION     DADLSA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.176"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..268)
                     /locus_tag="JN691_003623"
     CDS             complement(<1..268)
                     /locus_tag="JN691_003623"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244949.1"
                     /translation="MKVQILNNNCEVVWSYDIAAHVDQSGDSWANGKHQIMAGVVFSL
                     RRALEQAEVFPSGPEWKWPFSICPNSESTFQKIGQKVALEEHQPT"
     gene            complement(462..1136)
                     /locus_tag="JN691_003624"
     CDS             complement(462..1136)
                     /locus_tag="JN691_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HAY7244950.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            1227..1427
                     /locus_tag="JN691_003625"
     CDS             1227..1427
                     /locus_tag="JN691_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7244951.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            1471..2022
                     /locus_tag="JN691_003626"
     CDS             1471..2022
                     /locus_tag="JN691_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244952.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAAGTHYIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEVIEQIGNYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            2019..2855
                     /locus_tag="JN691_003627"
     CDS             2019..2855
                     /locus_tag="JN691_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HAY7244953.1"
                     /translation="MNSLTANNRLSQQLVVSVAEHLLLRHECRLPNHLAVSNHRELYL
                     TVGGELCRNLTAGFVTEEGFMSMLFVGSQKHSALSIFAKTTRMSALVLCGNSGVILLS
                     VKDHQHIDSAIPGRYTVQAPYKAGAGIGVLEMLSAIYDAPASFLSSASAHTQIMVGWT
                     GALKGAPVSDNAGYANPVQFTTSEIGVSGGEGNSLLSEAAIMATVPALTRLNDEDLHK
                     LSYVTTALRALRKVTLSDPQAHQVLVETLLNLQAERIRLAHKANFHIHRLLNISGGHR
                     HA"
     gene            2860..3084
                     /locus_tag="JN691_003628"
     CDS             2860..3084
                     /locus_tag="JN691_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244954.1"
                     /translation="MILNICRLLQRKKTSIPTVGQWYTTPAGHVLRVSLVDRECQKVV
                     CEPLGRNYRVSMPLIAFRSGKNMKHLGGAA"
     gene            3081..3898
                     /locus_tag="JN691_003629"
                     /pseudo
     CDS             3081..3898
                     /locus_tag="JN691_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3895..4389
                     /locus_tag="JN691_003630"
     CDS             3895..4389
                     /locus_tag="JN691_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY7244955.1"
                     /translation="MIMSLLNEVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQSVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLFRRAATVWMEAFRESHSQPERNNFLAHRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            4389..5042
                     /locus_tag="JN691_003631"
                     /pseudo
     CDS             4389..5042
                     /locus_tag="JN691_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
     gene            5039..5365
                     /locus_tag="JN691_003632"
     CDS             5039..5365
                     /locus_tag="JN691_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAY7244956.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRATHWLH
                     KRGLKV"
     gene            5362..5751
                     /locus_tag="JN691_003633"
     CDS             5362..5751
                     /locus_tag="JN691_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7244957.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKPAGAAA"
     gene            5771..6580
                     /locus_tag="JN691_003634"
     CDS             5771..6580
                     /locus_tag="JN691_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAY7244958.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            6624..7159
                     /locus_tag="JN691_003635"
                     /pseudo
     CDS             6624..7159
                     /locus_tag="JN691_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328142.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
BASE COUNT         1761 a         1681 c         1950 g         1767 t
ORIGIN      
        1 cagttggctg atgctcttcg agtgcgactt tctgaccaat tttctgaaat gtactctccg
       61 aatttggaca aatagaaaaa ggccatttcc attcagggcc tgatggaaag acttcagcct
      121 gttctaaagc acggcgtaaa gagaatacaa ctccagccat aatctgatgt ttcccattgg
      181 cccagctatc gccgctctga tctacatggg cggctatgtc gtatgaccaa acgacttcac
      241 agttattgtt taaaatctgg actttcattt catacacctg ctttaacatg agtacctagt
      301 ggcacaacat gactcaacga atcatcctgg acttcatatg ccccaggcgg ctacttcgtg
      361 ggcgtcctgc ctgtttgttg tttctcttgg gtacattatg tatctcatgg gtacattgtc
      421 aagtataaaa aaacctgccg aagcaggttc ataaacattg attaggcttt gattttgtat
      481 cttcttggtt ttcctgagaa aatcacagtt ccaattatag agcaattacc gttgatctta
      541 atgtaaggct caggccagtt tgggtttaac gctttgagat aacgctgtgt cccatcttct
      601 atcaaccttt tgaaggtggt ttcacctgta tcgtgcatca atgcaataac gtcgtcaccg
      661 tggcaggcag gtacttcagg atcgacaaaa atcatgtctc ccgggcggta ctcatcaatc
      721 attgaatcac ctatcacccg caagatataa gtcatttccc cacagggtac agggcaggga
      781 tacgtttctg ctgtgctcaa atcaacctca gaatatccaa cttctttcca tgctccggcc
      841 tgtacccatg atatgacagg gactaatgtg atttgtttat tagtgattga aacatcaggt
      901 ttttttgtga tgttcgttgt ctggtgttct tgatcgagcc atccgacagg caggtcgaaa
      961 catttttcga tgtgccgtgc catgctgtca ccgatatttt tagtagcgcc atcccccata
     1021 aacctgctgg tttgggttgg ctcgcgatca atcatggtgg caaaggatga atttccgcca
     1081 acaccatctc tcagttttct ggcgttagac cgccggatgt catggattgt tttcataacg
     1141 aaattaaaac ctttgtaccg ataaggtaca agtatcttga gggttcattt caatcatgta
     1201 atatgtatac cggaggtaca tattgtatga aagcgtattg ggactcttta accaaagaac
     1261 agcagggcga gttggccgga aaagttggct caacacctgg ctacttacgg ctggttttca
     1321 atggttataa aaaagccagt tttgtgctgg cgaaaaaact tgagcaatgc acgtcaggtg
     1381 caattacgaa atctgactta agaccggata tctatccgaa agattaacag aacaccttca
     1441 atttttaacc acagaacgat gaggctaacc gtgggtaagt atcactggaa agtagaaaaa
     1501 cagcctgagt ggtacgtgaa agctgtcaga aaaactatcg cagcgttgcc gggtggttac
     1561 gctgaagcag ctgactggct ggatgtaaca gagaacgcat tatttaaccg ccttcgtgcc
     1621 gatggcgatc agattttccc gctgggatgg gcaatggttt tacagcgcgc ggctggcact
     1681 cactacattg cggatgctgt cgcacagtct gctggtgggg tgttcgtatc gcttcctgaa
     1741 attgaggaag tagagaacgc cgatataaac cagcgcctgc tggaagtcat cgaacagatc
     1801 gggaattact caaagcagat tcgttcggca atcgaagatg gggtcgtgga gccacacgag
     1861 cagacagcaa ttaatgatga gttgtatctg tcaatttcga agctccagga gcatgcagca
     1921 ctggtctaca aaatcttttg cgctccagaa aagagtgacg cccgcgagtg tgcagctccg
     1981 ggcgtcgtgg cgttttgtgt ctgtggagaa actaacgcat gaacagttta acggcaaata
     2041 accgtttgtc gcaacagctg gtggtcagtg tcgctgaaca cctgttgtta cggcatgaat
     2101 gcagattacc aaatcacctg gctgtaagta accacagaga actttacctg actgtggggg
     2161 gcgagttgtg caggaactta accgctggtt tcgtgacgga agagggcttt atgtccatgt
     2221 tattcgttgg gagccagaaa cacagcgcgt tatctatctt cgcaaagact acccgcatga
     2281 gtgctttagt cctttgtgga aattcaggcg tgattttgtt gagtgtgaag gaccaccagc
     2341 acattgattc tgccattccg ggacgttaca ctgttcaggc accttataaa gcgggtgccg
     2401 ggattggcgt cctggaaatg ttatcggcga tatatgacgc gccagcgtct tttttatcgt
     2461 ctgcgtctgc gcacacccaa attatggtgg gctggacggg ggcactgaaa ggtgcgccgg
     2521 tttccgataa cgccggttac gccaaccccg ttcagttcac caccagcgaa attggcgttt
     2581 ccggtggtga aggtaattca ctgttatcgg aggctgccat catggctact gtcccagccc
     2641 tcactcgtct gaatgatgaa gacttacata aactcagtta tgtaacaact gcactacgtg
     2701 ctctgcgcaa ggtaactctt tcggatccgc aggcgcatca ggttctggta gaaacccttc
     2761 ttaacttgca ggctgaacgt atccgtttgg cgcataaggc taattttcat attcaccgtc
     2821 tcctgaatat cagcggaggg catcgtcatg cttaatccgt tgatcctcaa tatttgccgt
     2881 ttgcttcagc gtaaaaaaac atcaattcct acagttgggc agtggtacac cacgcctgca
     2941 gggcatgttc tacgtgttag cctggttgac cgtgaatgtc agaaggtggt ttgtgaaccg
     3001 ctgggccgta attaccgcgt cagtatgccg cttatagcct ttcgctccgg aaaaaacatg
     3061 aagcatctcg gaggtgcagc atgagtatgg agctgatggt taaagcgatg aaaattcgag
     3121 tgggtaatcc attgcgaaaa ctggttctga tcaagctggc tgataatgcc agcgatcagg
     3181 gtgagtgctg gcccagctac cagcatattg ctgaccagtg cgagattagc aaacgttctg
     3241 tgatgaatca tattgcggcc ctttgtgagt ccgggctggt aaaaaaagtc acccggaaag
     3301 gtgaaaaagg taactcaagt aatatctatc tccttcatct tgatggtgca ggagattcac
     3361 taggggtagt gcaaataatt tactatctgg tgcagcaaat tcactaggta gtgcaggagt
     3421 tgcaccaggg ggtagtgcag gagattcacc cagaaccagt cactcttttg aaccagtcaa
     3481 agaaccagtc aatgacccaa tagctgttgg tgcatctgct gatgagtctg tgcgagttcg
     3541 ttcaaaccga ccggaatact ctccggagtt tgagcaggca tggctggcct atcccaaacg
     3601 tgctggtggc aattcaaaat ctgcagcctt caaagcctgg aaagcccgtt tgaatgaggg
     3661 ggtaaacccc gaaaccatgc tggaaggtgt gaaacgctac gcgggctggg tatctgcgat
     3721 gggtaacagc ggcacacaat ttgtgaaaca ggctgtcacg ttctttggtc cggatcgtca
     3781 tttcgaagaa tcctgggaag ttcctgcagt atctgcagcc agacgcgagg acccgtactt
     3841 caaagccagt tacgacaacg tggactacag ccagatcccg gcaggattca gggggtgatt
     3901 atgagtcttt tgaatgaagt tcagaaattc attgaagccc atccggggtg tacttccgga
     3961 gacattgcgg atgcttttgc tggttactca cggcagcgcg ttctgcagtc agcaagcaag
     4021 ttacgtcaga gtgggcgtgt ggctcaccgt tgtgaaggag atacacgcag acatttcccg
     4081 cgcctgactg agagagcgca ggagccggaa ccacaatctg ttcgtgaaac cagacctgtg
     4141 cgcaatttct atgtcggcac taacgacccc cgggtgattt tgtgcctgac ccgccaggct
     4201 gaagaactgg agtccagggg cttattccgt cgagctgcaa cggtgtggat ggaggcattc
     4261 cgtgaaagcc actcccagcc agaacgaaac aattttctgg cgcatcgtga gcggtgctta
     4321 cggaaaagca gcaagcgcgc tgcatcgggt gaagagtggt atctgtcagg gaattacgtg
     4381 ggggcttaat gagtaataaa tattgccagg cgctagtgga gctgcggaac aaaccagccc
     4441 atgaactgaa ggaagtgggc gatcagtggc gcacgccgga caacattttc ttgggaatta
     4501 acaccctgtt tggcccgttt gttctggatc tgtttactga tggtgataac gccaaatgtg
     4561 ccgcttatta cactgcggaa gacaacgcgc tggcgcatga ctggtcagaa cgtcttgcgg
     4621 agcttaaagg tgctgccttt ggtaatcccc cgtacagccg cgccagtcag catgaggggc
     4681 aatacatcac cggcatgcgt tacatcatga aacatgccag tgccatgcgt gataaaggtg
     4741 ggcgctatgt tttcctgatc aaagctgcca ccagcgaagt ttggtggccg gaagatgcag
     4801 accatattgc ttttattcgc gggcgtattg gttttgaact gccagcctag tttatcccga
     4861 aggacgagaa gcaggtgccg acaggcgctt tcttcgctgg tgctattgct gttttcgaca
     4921 agacctggaa gggaccggca atcagctaca tcgggcgcga tgaacttgag gcatgtggtg
     4981 aggcctttct ggcgcaggtt cgccagcagg cggaaaaact ggtcagggag atggcggcat
     5041 gacgacatta actcaatgcc agcagcaggt gctggatatg ctgatttctt accagaaaga
     5101 acgtggcttc ccgccaacca atcaggaggt ggcaaccatg ctgggatacc gttcagtgaa
     5161 tgcagcggtg gagcatcttc gcgcactgga gaaaaaaggc gtcatcacga taaagcgtgg
     5221 cgtggcccgg ggcatcacgc ttcataccgc ggtgaaggac gacgacagcg aggcggtcgg
     5281 gattatccgc tcactgcttg ccggtgagga aaacgccagg ctgcgtgcaa cccactggtt
     5341 acataagagg ggcctgaaag tatgaagctg atcctgcctt tcccgcccag cgtgaacacg
     5401 tactggcgac accccaacaa aggggcgttt gcaggtaaga gcctgataag cgcggcgggg
     5461 cgaaaatttc agagcgcggc gtgcgcagca atagttgaac agttacgtcg tctgccgaaa
     5521 ccaacgtcgg cacctgcttc agtggagatc gtgttgtttc ctccggataa ccggatccgc
     5581 gatctggaca actataacaa ggcgctgttt gacgccttga cccacgcggg ggtgtgggaa
     5641 gacgacagtc aggtgaaaag aatgctggtg gagtggggac cggttatccc gaaagggaag
     5701 gtcgagatca ctatcagtaa gtatgagaaa ccggcgggtg cagccgcctg attaagagga
     5761 gaaacgaagt atgaataatc tgatggtcat tgatggtatt gaagttcgtc gtgatgctta
     5821 tgggcgttac agcctgaacg atctgcatcg cgcagcagta gcatctggtg caaatgccag
     5881 aaccaaggag ccaggaaagt ttctttccag ccaacaaact gttgagcttg ttcatgaatt
     5941 gaccaacacc cagaatttgg gtgttgaccc ggtgagtgtg attcatgggg gaaatgaacg
     6001 gggaacgtat gtctgcaagg aactggtgta tgcctatgca atgtggatca gcccgtcatt
     6061 ccatctgaag gtgatccgta ctttcgatat ggtaaccagc gcaccggaaa aattatccgg
     6121 gcaggctgct gacaagatgc aggctggtgt gattctgctg gactttatgc gtcgggaatt
     6181 aaacctgtct aactcatctg tgcttggggc ttgtcagaaa ctccaggagg ctgttggctt
     6241 accgaatctg gctccgcgct atgcaattga tgctcctgcc gatgcacccg atggctcaag
     6301 tcgccctacg ctgtcactga gtgcactgct gaagcagtat ggtatccgcc tgacggctaa
     6361 tcaggcatat caccagatgg tgaagctggg gatcgtcgag cagcgcgaac gatacagccg
     6421 taccgcgatt aacaacatca aaaaattctg gtcgctgaca gcgaaaggct gcatgttcgg
     6481 caagaacatc accagtcccg caaatccgcg cgagacgcag ccgcatttct tcgaatcccg
     6541 attccctgag ctgttaaagc tgctcgatac cgttcattga ggtgaccgtg agagcattac
     6601 tgacccctga aattgccccg cgtatgggga tcgtattgtt cagacccggt tcagagctga
     6661 tgcccctgtt tatgcagggg cgtgtcctgc tggagcctga gccggaacgt tattcatctt
     6721 ttgccagtgg tgccgttccg gcggcatcac aaccgctggc ggatgatcct gctgttcggg
     6781 ccgtgttccg caatgaggca gtgatccgtc gtgctggtgg cgtggaatgt cttgaaagct
     6841 ggttacttcg tgaaaaaggc tgctagtggc ctcattccga ctggcacagc gagaacatga
     6901 caacaatgcg acacgctccg ggcgcaatcc gtctgtgctg gcactgcgat aaccagctgc
     6961 gcgatcagtt cacggaacgg ctggaatcaa tggcaacgga taactgtgcc cgctgggtgt
     7021 tgtctgttgt gcgtcgggat ctcggttttg atgacagtca cgttgtgaca atgccggaac
     7081 tgtgctggtg gctggttcgt aatgatctgg cggatgcctt accggaaagc gcagcccgta
     7141 aggcactgag attaccgaa
//
LOCUS       DADLSA010000176         7131 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.177, whole genome shotgun sequence.
ACCESSION   DADLSA010000176 DADLSA010000000
VERSION     DADLSA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.177"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(32..1672)
                     /gene="pgm"
                     /locus_tag="JN691_003636"
     CDS             complement(32..1672)
                     /gene="pgm"
                     /locus_tag="JN691_003636"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAY7244959.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLSFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(1698..2243)
                     /gene="seqA"
                     /locus_tag="JN691_003637"
     CDS             complement(1698..2243)
                     /gene="seqA"
                     /locus_tag="JN691_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAY7244960.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            2428..3192
                     /gene="ybfF"
                     /locus_tag="JN691_003638"
     CDS             2428..3192
                     /gene="ybfF"
                     /locus_tag="JN691_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7244961.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            3263..3625
                     /gene="ybfE"
                     /locus_tag="JN691_003639"
     CDS             3263..3625
                     /gene="ybfE"
                     /locus_tag="JN691_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAY7244962.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            3765..4295
                     /gene="fldA"
                     /locus_tag="JN691_003640"
     CDS             3765..4295
                     /gene="fldA"
                     /locus_tag="JN691_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAY7244963.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            4505..4591
                     /gene="uof"
                     /locus_tag="JN691_003641"
     CDS             4505..4591
                     /gene="uof"
                     /locus_tag="JN691_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAY7244964.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            4584..5030
                     /gene="fur"
                     /locus_tag="JN691_003642"
     CDS             4584..5030
                     /gene="fur"
                     /locus_tag="JN691_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAY7244965.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(5114..5440)
                     /locus_tag="JN691_003643"
     CDS             complement(5114..5440)
                     /locus_tag="JN691_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244966.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(5477..6883)
                     /gene="chiP"
                     /locus_tag="JN691_003644"
                     /pseudo
     CDS             complement(5477..6883)
                     /gene="chiP"
                     /locus_tag="JN691_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         1732 a         1822 c         1774 g         1803 t
ORIGIN      
        1 cttgcgaccg cccttttttt attaaatgtg tttacgcgtt tttcagaact tcgctaacaa
       61 tctcaaccgc ttctttctca atctgcttgc gatgttcttc accgaggaag ctttcgcagt
      121 agatcttata tgcgtcttcc gtgcctgacg gacgcgcggc gaaccagccg ttgtcagtca
      181 tcactttcag accgccaata gaagcaccgt tgcccggagc agcagtcagg cgcgcggtga
      241 tcgggtcacc tgccagggtg ctggcgctca ccatttccgg agacagctta gacagcgccg
      301 ctttttgtgc ggaagtcgca gctgcctgca aacggttgta gctcggcgca ccaaagcgtt
      361 ttgccagttc gttgtagtgt tcctgcgggt tcttaccggt gacagcggtg atttccgccg
      421 ccagcagaca cataatgatg ccgtctttgt cggtggacca cggcgtgccg tcgaaacgca
      481 ggaaggaagc ccctgcgctc tcttcgccgc caaagccgaa gctgccgtcg aacagaccat
      541 caacaaacca tttgaaacct accggtactt ctaccagctt acggcccaag tcattgacca
      601 cacggtcgat catcgcagaa gaaaccagcg ttttaccgac agcaacatct ttgccccact
      661 gcggacgatg ctggaacagg tagttgatcg ccaccgccag gtagtggttc ggattcatca
      721 aacctgccgg agtgacaata ccgtgacggt cataatccgg gtcgttagca aacgacagat
      781 cgaacttatc acgcagtgcc agcagacccg ccatcgcaca ctcggaggag cagtccatac
      841 ggatcgcacc gtctttatcg aggtgcataa agcggaaggt ttgatcgacc tgatcgttaa
      901 cgatggtcag gttgaggttg taatactcgc caatgcgctt ccagtattcg ataccggaac
      961 cgcccaacgg atcaacgccc agcgtcaggc ccgctttctg aatcgccgcc atatcaacga
     1021 tatcggccag cccttccacg aacggctgca ccagatcctg ctctttcaca tggccggatg
     1081 ccatcgcttc gtcgagggag atacgcttca cgcctttcag gccatcggcc atcagtgcgt
     1141 tggccctgtc ttccaccact ttggtgacgt tagtatcagc cgggccacca tttggcggat
     1201 tgtacttgat accaccatct tccggcgggt tatgggacgg tgtaatcacg ataccgtctg
     1261 ccagcgggcc acctttttta ttgtgaacca ggatggcatt ggaaacggca ggcgtcggag
     1321 tgaagccatt gttttcctgc acaatgacat caacgccgtt cgctgccagc acttccagta
     1381 cggaaatgaa cgcaggctcg gacagggcgt gagtatcttt acccacatag caagggccag
     1441 tgatgccgtt tttcgcacgt tcttcagcaa ttgcctgagc gatcgccaga atgtgcggct
     1501 cgttaaagct gtggcgcgct gcactgccac ggtgaccgga agtaccgaat ttcaccgcgt
     1561 gctccgcatt ccctgcttct ggtttcagta cataatattg cgccgtcagt tgggcgacgt
     1621 taatcaaatc actctgttgt gcaggttggc ctgcacgatt gtggattgcc attgctttgt
     1681 cctttgtctg caacgtttta gatagttccg caaaccttct caatcaattc cgccgggaat
     1741 tgcatcgact gcatgatgtg ttcgatcatg ctgcatttac ggccggtgtt ggtgttggtg
     1801 atcacccaat acggcgtgcc tggcacatgt ttcggcttgg tctgattacc atttttcagc
     1861 agcgtttgtt catctgccgc aaagtacacg cgtgtacgac cgtgcaacga ttccgttgct
     1921 tcggcaaacg cctgggcgtc aagagaatat agtgtagaca acagcagcat aaagcgattg
     1981 accgctcgct tttgctctgc gtattcgtcc gaaagcagaa gttcacgcat tgcgcgaacc
     2041 ttgtctttaa tcgttttgac cggcttcgct tcgacgatag caggtgacgc aacgcgaacc
     2101 tctttcgtca ccggagcagc aggctgtgat gcggcggaaa atttcaacat acgccgtaaa
     2161 atgtcggatg cgctctcgcc gatatgctta gtgtggctgg caatatagct gtagagttca
     2221 tcatcaactt caatcgtttt catcttaatc cagtgcagtg tcttagctga atacaagtcg
     2281 ccaagagtat atgaataaat cccggcagcg gatagcgtca agcctggctt gcagaaaaac
     2341 tcggaataaa ccttatttgt tgcagccctg tggcacaatg gtcaacataa taccctaacc
     2401 cgacaacgcg aaaaaaagaa ctttgccatg aaattaaata tccgcgcgca aactgcacaa
     2461 aaccagcaca ataattctcc catcgttctt gtccatggtc tgtttggtag cctcgacaac
     2521 cttggcgtac tggctcgcga tctggtaaac gatcacaata tcatccaggt tgatatgcgt
     2581 aaccacggtc tttcaccgag agagccggta atgaattacc cggcgatggc gcaggatctt
     2641 gttgataccc tggatgcact gcagatcgac aaagcaacat ttatcggtca ctccatgggc
     2701 ggtaaagcgg taatggcact tactgcactg gcccccgatc gcatcgataa actggtggcg
     2761 atcgatatcg caccggtcga ctatcacgta cgccgtcatg atgagatttt cgcagctatc
     2821 aacgcggtca gtgaatcgga cgcacaaact cgccagcaag cagcagcaat aatgcgccag
     2881 catcttaatg aagaaggggt gattcagttt ctgttgaaat cttttgttga cggggagtgg
     2941 cgctttaacg tgccggtatt gtgggatcag tatccgcata ttgtgggttg ggagaaaatc
     3001 ccggcatggg atcaccctgc cctgtttatc cctggcggca attctccgta tgttagcgag
     3061 cagtaccgtg atgatttact ggcacaattt ccacaggcac gggcgcatgt gattgcaggc
     3121 gcgggtcact gggtccatgc tgaaaaaccg gatgcggtat tacgcgctat ccgtcgctat
     3181 ctcaatgatt aacgcaactg attaaaaacc cagcgtccgc gtcgctttcc gcgcgtgggc
     3241 gttggcgtga ctcatcgcgc tgatgtatta tggcgcgcta tccatccggg cagaggcctg
     3301 gctgattgtt tcccccgaag tcaccaagat catggccaaa gaacaaacgg accgtacgac
     3361 attagatctg ttcgcgcacg agcgtcgacc gggacgaccg aaaactaatc cgctttcgcg
     3421 cgatgaacag ctgcgtatta ataaacgcaa ccagctaaaa cgcgacaaag tacgtggcct
     3481 taagcgtgtc gaactgaagc tgaacgcgga agctgtcgag gcgctgaacg agctggcgga
     3541 atcgcgtaat atgagccgta gcgaactgat cgaagagatg ttaatgcagc aactggcggc
     3601 gctgcgtagt cagggcatcg tttaaatttc cactttcatg tagcacagtg tgcagtcctg
     3661 ctcgtttgct gattccgcaa gactgcctgt tctgctatga ttgcctttat ccgtgggcaa
     3721 ttttccaccc ccatttcaat aagtttcaag aggttatttc actcatggct atcactggca
     3781 tctttttcgg cagcgacacc ggtaataccg aaaatatcgc aaaaatgatt caaaaacagc
     3841 ttggtaaaga cgttgccgat gtccatgaca ttgcaaaaag cagcaaagaa gatctggaag
     3901 cttacgacat tctgctgctg ggcatcccaa cctggtatta cggcgaagcg cagtgtgact
     3961 gggatgactt cttcccgact ctcgaagaga ttgatttcaa tggtaaactg gttgcgctgt
     4021 ttggttgtgg tgaccaggaa gattacgccg aatatttctg cgacgcattg ggcaccatcc
     4081 gcgacatcat tgaaccgcgc ggtgcaacca tcgttggtca ctggccaact gcgggctatc
     4141 atttcgaagc atcaaaaggt ctggcagatg acgaccactt tgtcggtctg gctatcgacg
     4201 aagaccgtca gccggaactg accgctgaac gtgtagaaaa atgggttaaa cagatttctg
     4261 aagagttgca tctcgacgaa attcttaatg cctgatgtga tgcggcgtag actcatgtct
     4321 acgccgtatt aatagataat gccaatcaaa ataattgcta caaatttgta acttttgctg
     4381 ttgtacctgt acaatgtccc agtgttcaag tggccttgcc gttgtaaatg taagctgtgc
     4441 cacgttttta ttaacaatat ttgccaggga cttgtggttt tcatttaggc gtggcaattc
     4501 tataatgata cgcattatct caagagcaaa ttctgtcact tcttctaatg aagtgaaccg
     4561 cttagtaaca ggacagattc cgcatgactg ataacaatac cgccctaaag aaagctggcc
     4621 tgaaagtaac gcttcctcgt ttaaaaatcc tggaagttct tcaggagccg gacaaccatc
     4681 acgtcagtgc ggaagattta tacaaacgtc tgatcgatat gggtgaagaa attggtctgg
     4741 ctacggtata tcgcgtactg aaccagtttg acgacgctgg tatcgtcacc cgccacaatt
     4801 ttgaaggcgg taaatccgta tttgaactga cacagcaaca tcaccacgat cacctgattt
     4861 gcctcgactg cggcaaggtt atcgaattta gtgatgattc catcgaagcg cgtcagcgtg
     4921 aaattgccgc aaaacatggc attcgcctga ctaaccacag tctctatctt tacggtcact
     4981 gtgccgaagg cgattgccgc gaagatgagc acgcgcacga aggcaaataa gccagcctga
     5041 acgagaaaag ccaacctgcg ggttggcttt tttatacaag ggaaataaga aaagcgagag
     5101 ttacagctct cacttatttg ttattactgc gtatttcctg ccagacctta tcacaatcgg
     5161 ctttcaccgc ctgatcattt ccggtttgcg ttgcacgcag acactgctga taatccagta
     5221 cgcgtacact ttcctggtcg gcaaactgct gatgttgttt ctctttcttc agcacgttta
     5281 acacgctctg gcaggcttca attttttctg gcgaaccttg cgcagtgttg atacaggcgc
     5341 tgtatgcctc tttcagacgg ctgtcttcct gtggtgcggt tgattgcgca caagctacca
     5401 gccccgatgc catcatggcg atgagaatca gttttttcat tgaatgcggc aggcacaaaa
     5461 cctgccgcgt cgggcatcag aagatggtga atggtgcgat taccataaat ttcacgtcac
     5521 gctcatcctg gaagatgtta ccgtaaccgc cgccccagct tgggatgtcg gagtggttgt
     5581 cgtattcggt gaagtgcagt ttgaacatcg tgcctttggc gcgaccgtcc tgaatggtgt
     5641 agaccgcatc caggctgtag gcagactctt caatagtccg ttttttgtcg tagtacgcat
     5701 ccggattgct ctgccaggtc gcaggtttag catcccatgc gtaaacgtag gaagcgccga
     5761 tggcgaagcc tggaagattc cagtttttca ggtcatacat cgcaccgaag aagaccgctt
     5821 tttcgccgtt agcgttgaag tcggaacggt tatcccacca gatatccagg cggccgttgg
     5881 aggaagcgta ggttggggtc atacgttgca ggaagtatcc ctgctgactg tcagccttaa
     5941 cccaggtgcc ttcgaggcgc aaatccacta cgtcagccgc ccggtaacca aaggtcaacg
     6001 cttgcagcta ggcggtgccg tcataaagat cgttgacgct gcgatcgtca actttatcgc
     6061 gggtaccata gaactggtag ctggtggtta acgggctacc ggcgatatca aatttgtagc
     6121 tggctttggc aaaatattga tcgatatacc cttccgcctg accaaatgcc gcttccagta
     6181 cgaagttatt tttgaagtcg tatttcgcac cgatggagtg cagataatca actttggtgg
     6241 ttttatcgtt ctgataaaac tcatccattt ccagatgcca cggcgctttg tattcgttgg
     6301 tccacatgta ggagaaactc aaggcaccag catcgccgta atcaaaattc gccccggctt
     6361 ccgccccctg ataagtaccc ggcataaagc tccagtgcgg cgctaacagc gtttgaccgg
     6421 ttggctgaat gtaacctgcc ctcgcccaaa ccggaccgta tttaaatttg gccgcagctt
     6481 tatacaggct tataccgctt ttgtcgccgg accagtcttc gtcataggct ttattacttt
     6541 ttgaaaacgc gatttcgttc gggtgggagc tgtcgccgtt ttccgccatt tcaatcgccg
     6601 taaacgcagc aatatcaagg ccgaacatat cagcagcata accggactga aaatagaggt
     6661 tggcattcca ggtggagtga gaaaggttgg ttttgtattt gtcgccgtcg gtaacatctt
     6721 tacggtcgcg ttcacgctgc cagtaataga taccgccggt taaggttgaa tcgtcgatga
     6781 atccttcggc gcgagcctcc ggcattgcca taaagcccga cattgctgta acaccggcaa
     6841 tagccagcgc cagcgtacta cgtttgccac taaacgtacg catgggttaa tcctctttga
     6901 cgtataaatt gctgcaccaa aggtgaacag cgaaaaaaaa taaaaattaa aaaagtagcg
     6961 ttgagaaaag tctcgctgac tacaggaaca gactattttt cgcgacgcga attatcaatc
     7021 tctttctgta accagaatgt aagaacatga gcaaccgcac atattttgat gatttttgtt
     7081 acaaggctta agtttgcggt tgaaaggctt gcagaagaat aaaaacgtga a
//
LOCUS       DADLSA010000177         7126 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.178, whole genome shotgun sequence.
ACCESSION   DADLSA010000177 DADLSA010000000
VERSION     DADLSA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.178"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            154..1197
                     /locus_tag="JN691_003645"
     CDS             154..1197
                     /locus_tag="JN691_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAY7244967.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            1712..2173
                     /locus_tag="JN691_003646"
     CDS             1712..2173
                     /locus_tag="JN691_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244968.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTDPTHVMWTRP"
     gene            complement(2170..3025)
                     /locus_tag="JN691_003647"
                     /pseudo
     CDS             complement(2170..3025)
                     /locus_tag="JN691_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3022..3321)
                     /locus_tag="JN691_003648"
     CDS             complement(3022..3321)
                     /locus_tag="JN691_003648"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7244969.1"
                     /translation="MTKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYES
                     QLYAWRSKQQQQMSSSERESELAAENVRLKRQLAEQAEELSILQKAATYFAKRLK"
     gene            3783..4040
                     /locus_tag="JN691_003649"
     CDS             3783..4040
                     /locus_tag="JN691_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244970.1"
                     /translation="MKNYPTYGFYYPIANFIALQINGSKTGFAASITGFTQTISAGTI
                     SFLSVTVSDNGVGPGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            complement(4681..4953)
                     /locus_tag="JN691_003650"
     CDS             complement(4681..4953)
                     /locus_tag="JN691_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244971.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVLLLQLA"
     gene            5042..5221
                     /locus_tag="JN691_003651"
     CDS             5042..5221
                     /locus_tag="JN691_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244972.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            5273..5467
                     /locus_tag="JN691_003652"
     CDS             5273..5467
                     /locus_tag="JN691_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244973.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(6266..6602)
                     /locus_tag="JN691_003653"
                     /pseudo
     CDS             complement(6266..6602)
                     /locus_tag="JN691_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2069 a         1601 c         1508 g         1948 t
ORIGIN      
        1 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
       61 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
      121 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
      181 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
      241 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
      301 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
      361 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
      421 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
      481 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
      541 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
      601 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
      661 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
      721 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
      781 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
      841 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
      901 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
      961 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
     1021 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
     1081 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
     1141 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
     1201 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
     1261 cttccccaag tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
     1321 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
     1381 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
     1441 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
     1501 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
     1561 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
     1621 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
     1681 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
     1741 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
     1801 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
     1861 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
     1921 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
     1981 tgttacaccg atagtgacgc cagccttgct aagccgtgag ggttttttat atccgacgcc
     2041 agcgacctcc attcccagag acctgcttga acctttgcca aaaggagcat tttcgattgg
     2101 tgagattggg cagtatgaca aatacaagac gacccatact gatcctaccc acgtaatgtg
     2161 gacacggccc taagcgagat tatggttttc aaattgttcc gggctgagac cgccacaggc
     2221 actgtgacga cgccagcgat tgtaatcaca ctcgatataa ttaaacaccg ttgcccgcat
     2281 tatttcccgg ctgctaaagc gttccccgtg gatacattcc accttcagcg aatgaaagaa
     2341 gctttccaca caggcattgt cataacagta gcctttcgca ctcatactgc cacgcaggtt
     2401 gtgtcgcttc agcattgcct gataatcccc tgaacagtat tgaccaccac ggtccgtatg
     2461 aacaatgacg ctttccgggc gtcttctccg ccacaacgcc atttgcagtg catcacaggc
     2521 cagttgtgct gtcattcgcg gtgacatcga ccagccaatg acggcgcgtg accacaggtc
     2581 gatgactact gcgagataca accagccctc atcggtacgc aagtacgtga tgtcacccgc
     2641 ccacttctgg tttcgggcca ctggcgctga agtcctgctc cagcagattc tccaatacgg
     2701 gcaggccatg tgcacggtag ctgaccgggc tgaacttccg gctggctttc gcccgcagcc
     2761 cctgacgacg caggctggcg gcaatggttt taatattgaa ctccggcagt tcgtcagcaa
     2821 ggcggggagc accgtatcgc tgctttgcct caaagaatgc cttatggaca gcggcatcgc
     2881 aggtgagccg aaactgttgg cgcaggctca tctggtgacg acgcctgagc cagacatacc
     2941 agccgctgcg ggcaacccga agtacacgac acatcgcttt aatgctgaac tctgcccgat
     3001 gattttcgat aaagacatac ttcatttcag gcgcttcgcg aagtatgtcg cggccttttg
     3061 gaggatggac agttcctcag cctgctccgc cagttgtcgt ttaaggcgga cattttcagc
     3121 ggccagttcg ctttcgcgct ctgacgaact catttgttgc tgctgtttac tgcgccaggc
     3181 ataaagctga gattcataca ggctgagttc acgggctgcg gcggccacac cgatgcgttc
     3241 agcgagtttc agggcttcgt tacgaaattc aggcgtatgt tgtttacggg gcttcttgct
     3301 gattgatact ggttttgtca tgagtcacct ctggttgaga gtttactcac ttagtcctgt
     3361 gtccactatt ggtgggtaag atcaccctcc attaaaacta aaacgggcgc tttctgatat
     3421 atcatctgta agcgcagtaa taccattagt agcacctctc ggcggcgcat tgattattga
     3481 atattataac tggaatgtta tatttcttgt tatgatgctg ttcagtatta taataatgtt
     3541 attatcacca aagataactc atgcaaatga gaaagtaata tgtcaaaaca gtgcatcctt
     3601 tgtgaccaaa gagtttatct gcggcactat acttgtctca ttaatactca gttctctatt
     3661 ttgctacata tcaatatcac catcaatttt catgcatact tatggcctga gtacttttag
     3721 ttactcaatt attttctcct tctcagtttt atttttcata atcggaaatc aattatcaaa
     3781 gattgaaaaa ttatccaacc tatggttttt attacccaat agccaatttt atcgcattac
     3841 aaataaatgg ttcaaaaaca ggatttgccg catctattac aggttttact caaacaataa
     3901 gcgctggtac aatcagtttc ctgtcagtaa ccgtaagcga caacggtgtt ggtcccggga
     3961 ctactttagg tcttggttca ctaataactt cattactgtc aatagccgta atcgccattg
     4021 caggaaataa taaaaagtaa gtgtactctg actgcgtatg tatctacttt gtgtaatgca
     4081 ggtgtctatt gaaaataatt cttttcgtgg gcatctgttg tataaaataa aaaattatcg
     4141 taagtactcc atctgcgatg tcttgtgaaa attatcctgt ctggcaacaa tcgcgtccat
     4201 ctgtattaat ggactcgaac gatgaactct caaacaacac tagatatcac cggctttcgc
     4261 acttatttgc cagaatcatt acgtctcaga ttcgacgagc aattatctgt ccgggctatc
     4321 gcacagcagt taagactcag ttattccccc attcacaatc tctttcaacg aattaacgcc
     4381 tcaggtattg tctgaccatg acccgagtcc ctttcattcg cccaaccgat accatacttt
     4441 atgccaacag aaagaatgaa tgaaccgcgc ctaaaatcag cgaggacaca tggcacaaag
     4501 tgcgacatgt ctcacattta accccagtgg gggcaacaag tataaaaccg ttttttgatt
     4561 aagtggtcgg atatgaaaag gggaaggttt atgtgaaaaa tacctgtgtt ttctttgaaa
     4621 acacacaaca gtatcaggtt atttatgttt atacagtgcg ttccaaactg acctgaataa
     4681 ttacgcaagt tgcaacaata aaacattttt gccgtagtgt gaggtactcc ggtaaattgc
     4741 tttccaggtg cagacaatga ctaacgaaga actacaggca atcgcattat ccatgcttga
     4801 gcgccagcgc gaaaaagtac accagcaacg agagtctttg cttaacccat ctccattcac
     4861 caggccaaaa cgattggagc ttgaagcatt cttggacaaa tacccacggc ggcttaaatc
     4921 aggcaaaacc agaccaccag gctgatagct cacagtttgt tctccgattc aataaaacgg
     4981 ataagaacca tcagcgcggt agcgtaaggc actacagcgc attgctttac gagtaataac
     5041 aatggattta tcaccagaag acgcaaagtt tttacgagaa ctgcaacacc gtatgctcag
     5101 agaagccatt atcgaacagc aaacgggcat gagcgccata gacccattgc gatacaaacg
     5161 ggcctcatct cctttagttg acgacaattc caatgcggat aaaacacgtc caccacactg
     5221 attacatttt tcattagcac agtggtgtga tgcagcatac gaggaaaacc ggatgaatga
     5281 gaacaaaata aaaaggctgg agcaactgtt acaggcacgc caacgggagt ttgccactaa
     5341 atggggacgt gccgcgctgc cacatgaacg cctaacctat tttccacttg aagacctcat
     5401 taaaaataat ggcaaaaaaa tcaggtctga taagaaacac ttcttagaat gcaatgaaga
     5461 caaataacaa caatctgcat attgccacca accacagatg tggcatcgtt atcagcgcac
     5521 ttgattttct ttatatccac tgacgagctt gccggggcac ctttgtttgc cggtcattct
     5581 ggtatcatgc agatatttac ggcgtacaag cccactagca tgacattgta ataaggcctg
     5641 agcctaacgc tttaatgtgt gtcatacact cagtggcgta atcgttgaca atatggtgca
     5701 tgtaatcgta agcgtccagc gaaatccatc tggtcataat ctgaagcatc cgacaaagtg
     5761 gtgtccacca aataagtgat ctgcgcccaa tatcctgagc cggaactttg acaaaggcca
     5821 acgaaaaatg ggagcggatg ttacagagtt ctcggtacag gggaaaagtg gtacctgtcg
     5881 ccagttctcc gatctgttta accgggaaat tatctcctac agcttgtcag aaaggtcggt
     5941 gatggagcat cgttaatacg atgctatacg atgcattcac agtgcccggc cagaggatgc
     6001 cccgtcgctg catatggatc agggttggca atatcgaatc gcaggctata ggcaaagtta
     6061 aagccccatg gagtagcaca aaatatgccg cacaaaggaa acggtctgaa taacgcagtg
     6121 atgaagaact tcttcagcac actgctaaaa cgcatagtga tcgagcgctg attctggcga
     6181 acaactgaac taatacatca gaatctgcat tatgttaaat aaatatgaaa agatggttta
     6241 aataccccgt tacttttgac ttacactata cggtatcgca tcgtttaata ttcgcaccgg
     6301 ccagattttt atttctatta gttgtcacaa tactgaatgc gtacgaccac agtattctgg
     6361 ctcctgtgtg gttatgcttt aattctgcgt tccgggcaga taagcagttg cttgcaggaa
     6421 tccttcttgt gttaatgtca gttccccttt ttaccagtgc tgatttccac attccgtcca
     6481 acagagctta tagcctttcc ctggattata gcattgtccg gctgaagttc tttttgaata
     6541 ataatagaag cactgctggc agatccagtc cgtttttcat aacccactgt actgataacc
     6601 ataatctaat cagtagaaat tgagtcaaaa ataagcacta ctccatacag gataattaga
     6661 gatcagtttg attattcaca attcatcatc agcattttct atttctgacg aaatcaacat
     6721 gaaaataacc atatatgata attattataa taacggtttt aattggaata catatattac
     6781 aacgtattat atataattgg tattctggga actatattct caaaatacag tagaaacgag
     6841 gtatgtttct ggtggaaagg acagttggat taaaaagtaa gaattgataa aaaaaaacgc
     6901 cagcaacaca tagtgccggc ggagggaata ccccatggag aaaatgtgat gcctagagca
     6961 tcatgaatat ataattaaaa atagttagcg ttgtcaatac tttcacaaaa ataattttcg
     7021 tagtatagaa agatattttt atgcatgacc tacctgaatt tgctccgggt agaggttata
     7081 aataaaaatt gaatcacgac aaacacaata ttcatagtat ggcgat
//
LOCUS       DADLSA010000178         7124 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.179, whole genome shotgun sequence.
ACCESSION   DADLSA010000178 DADLSA010000000
VERSION     DADLSA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.179"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..273
                     /locus_tag="JN691_003654"
     CDS             <1..273
                     /locus_tag="JN691_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="allows for ions and hydrophilic solutes to cross
                     the outer membrane; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAY7244974.1"
                     /translation="QFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVG
                     ATYYFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            414..848
                     /gene="uspF"
                     /locus_tag="JN691_003655"
     CDS             414..848
                     /gene="uspF"
                     /locus_tag="JN691_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAY7244975.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1024..1959
                     /gene="ttcA"
                     /locus_tag="JN691_003656"
     CDS             1024..1959
                     /gene="ttcA"
                     /locus_tag="JN691_003656"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAY7244976.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2088..3461)
                     /gene="dbpA"
                     /locus_tag="JN691_003657"
     CDS             complement(2088..3461)
                     /gene="dbpA"
                     /locus_tag="JN691_003657"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAY7244977.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3939..4922)
                     /gene="zntB"
                     /locus_tag="JN691_003658"
     CDS             complement(3939..4922)
                     /gene="zntB"
                     /locus_tag="JN691_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAY7244978.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGSLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            5177..6409
                     /gene="dgcM"
                     /locus_tag="JN691_003659"
     CDS             5177..6409
                     /gene="dgcM"
                     /locus_tag="JN691_003659"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HAY7244979.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLTA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHNPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARPLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6430..6993)
                     /gene="smrA"
                     /locus_tag="JN691_003660"
     CDS             complement(6430..6993)
                     /gene="smrA"
                     /locus_tag="JN691_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HAY7244980.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKAAQAKQENWERHAKRSR"
BASE COUNT         1850 a         1825 c         1678 g         1771 t
ORIGIN      
        1 cagtttgatt ttggtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
       61 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
      121 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
      181 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
      241 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
      301 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
      361 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
      421 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
      481 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
      541 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
      601 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
      661 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
      721 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
      781 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
      841 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
      901 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
      961 gctgggcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     1021 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     1081 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     1141 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     1201 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     1261 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     1321 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     1381 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     1441 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     1501 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     1561 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     1621 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     1681 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     1741 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     1801 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     1861 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     1921 ttggatgaat tacggctgaa tgtggttgaa gtgaaataac caggatagcg cccgatgcgc
     1981 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatccggca
     2041 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     2101 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     2161 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     2221 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     2281 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     2341 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     2401 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     2461 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     2521 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     2581 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgatcgcg ttgctccaga
     2641 tcgccgtgta acgacaatgc actttgctct acttcattca gcgcgtcgca gacagcctgg
     2701 caatcttttt tggtattgca aaacaccaca caagaggatg gctgatgcaa gcttaataac
     2761 cgttgcaaca gaggaatttt gcctttactg gatgtctcat aaaattgttg ttcaatgggt
     2821 ggcaaagcat ctgttgagtc aatttcaatc gccaaaggat cgcgttgcac tcgtccgctg
     2881 attgcggcga tggcttccgg ccaggttgcc gaaaacagaa gcgtctgtcg agatgcaggc
     2941 gcaaaacgga tgacatcatc aatggcatcg ctaaatccca tatccagcat gcggtcggcc
     3001 tcatccatca ccaaagtatt cagcgcatcc agtgataccg tgcctttttg caagtgatcc
     3061 agcaaacgac ccggcgttgc cacgataata tgcggcgcat gttgcaacga atcacgctgc
     3121 ataccgaacg gttgaccacc gcacaacgtc aaaattttgg tatttggcag aaaacgcgcc
     3181 agccgacgca attcacctgc cacctgatcc gccagttcac gcgtaggaca cagcactaaa
     3241 gcctgggttt gaaatagcga cgcatcaatt tgctgtaaca agccgaggcc aaaagccgcc
     3301 gttttgccgc tgccggtttt cgcctgcacg cgaacatctt ttccggcaag gatcgccgga
     3361 agcgcggcgg cctgcaccgg cgtcatggtt aaataaccca actcattaag gttcgtgagt
     3421 tgggcgggag gcaaaacatt cagggtagaa aaagcggtca caatctattc tcgtggtcat
     3481 cgacgcaaag ttagcaggcg cgtatcctcg cagatctacg ctcacgatgc gacaatttaa
     3541 tcggttcttc atcgggtggt gggtcaggca tgggttgcgg gcgagggatc ggatcgggca
     3601 ctggaacagg atcgccagga atcggttcag ggacaggaat ttgcaaataa ataagtgtcg
     3661 tcatatttcc ctccggtcat tgggtggact cttaaagggt agacgctgat aaataacagg
     3721 caaaaaaaag ccgactcatc aaagtcggcg tcgtacgaat caattgtgct atgcagtaat
     3781 tcaaaaaagg aagtaagaca atatggagcg caacgcccat cgcttgacgt tgcattcacc
     3841 tgcaagggag atattgccct gaatgggtag agagtttatt gacttcgctc aaactttgcg
     3901 gcgtttttgt atacagacag ccggaaaaat tgcttttgtt acaaccattt actacggtgc
     3961 aaccataaag caacaccacc aataagaaca actaacagaa tacaaaaaat tgaaaatccg
     4021 aattgccacc cgccgccagg gatcccacca aggttgacgc caaataaccc ggtcagaaag
     4081 gtactgggta aaaagaccat tgccatcaac gacattgtat aggtacgacg agctaaattt
     4141 tcctgcatca cctgagcgat ttcatccgcc atcacgccag tccgtgctat acaggcgtcg
     4201 atttcgtcaa ggccgcgccc aaggcgatcg gcaatatcct gcatccgacg gcgttggtca
     4261 tcgctcatcc acggcaaacg ttcactggca agacgagcat aaacatcacg ttgcggtgcc
     4321 atatagcgac gcatcacaat taattgtttg cgcagcagag ccaggaatcc acgcggtgga
     4381 atttgctgat caaggagatt atcttcaagg tcgataattt tatcgtgcag ctgctcgata
     4441 aattcactgg aatgatcggt caacgcatcg cacacatcca ccagccatcc cccgcaatcg
     4501 gtcggacccg tgccctcttc cagatcgctc accacatcgt ccagcgccag cactttgcgt
     4561 tgtcgggtcg aaacaattaa ccgcccgtcc atatatacac gcatggcgac cagttgatcg
     4621 gggcgttcat cggtgctgcc gtttatacag cgcaatgtaa tcagcgagcc ttcaccgaaa
     4681 cggctgactc ggggccgtgt gctctcgccc gccagcgcat cacgtacgtt attgggaagc
     4741 agcggtgttg tcgccagcca ttgggcgcta tcatggtgta cataattaag gtggagccag
     4801 cagggatgcg cttcatcaat cacatctgta ttttccagcg gtttaacgcc gcctctacca
     4861 tccagcatcc aggcaaatac tgcatccggg acattaacgt ccgatccctt aatcgcttcc
     4921 acagtgcctc catcatcaac gcattatttt gtagtctagc cttctggccc tgttacgcaa
     4981 catctcatca ccccattacc ctgaaatgat taataaaatt ctgtctaaat tgaatacaaa
     5041 aagcaaaatg cttttccgta tacaaaccgt gtgaagtgtt aaatagcgtc tatcattatc
     5101 agaattatct gatcatatga cgtggctttt ttgcgatcgg atagcaacaa aaattgataa
     5161 aaataacggg atctcaatga ttacgcacaa cttcaatacc ctggacttac tcaccagtcc
     5221 tgtctggatt gtttcgccct ttgaggaaca gttaatttat gccaatagcg cggcgcgact
     5281 gttgatgcaa gacctcacgt ttagtcagct acgaaccgga ccctattccg tctcctcaca
     5341 aaaagaactg ccgaaatacc tctccgatct gcaaaaccaa cacgatatta tcgaaatcct
     5401 cactgttcag cgtaaagaag aggaaacagc attaagctgt cggcttgttt tgcgagagct
     5461 gacagaaaca gaaccggtga ttattttcga aggtatcgaa gcgccggcaa cgctgggttt
     5521 aaaagccagt cgctcggcaa attatcagcg caaaaaacaa ggtttttatg cgcgcttttt
     5581 tctgactaac tctgcaccaa tgttgttgat tgacccgtca cgagatggac aaatcgtcga
     5641 tgctaacctc accgcgctca atttctatgg ttataaccat gaaacgatgt gccagaaaca
     5701 tacctgggaa ataaatatgc ttgggcgtcg cgtcatgcct atcatgcatg aaatttcgca
     5761 tttacccggc ggtcataacc ctttgaattt tgttcataaa ctggcggatg gatctactcg
     5821 tcatgtgcag acctatgccg gaccgattga aatttatggc gacaagctca tgttatgtat
     5881 tgtgcatgat attactgagc aaaaacggct ggaggagcag ctggaacatg ctgctcacca
     5941 tgacgcgatg accggattac tgaatcggcg acagttttat cacattacgg aaccaggcca
     6001 aatgcagcat ctcgccatcg ctcaggatta cagcttgttg ctcatcgaca ccgatcgttt
     6061 taaacacatt aacgatctct atgggcattc taaaggtgat gaggtgttat gcgccctcgc
     6121 ccgccccctc gaaagttgcg ctcgcaaagg cgatttggtg tttcgttggg gaggcgaaga
     6181 gtttgtctta ttgctaccaa gaaccccact ggataccgcg ctttcgctgg ctgaaactat
     6241 ccgcgtaagc gtggcaaaag tgagtatttc gggcttacca cgctttaccg tcagcattgg
     6301 tgtggcgcat cacgaaggaa atgaaagcat cgatgaactg tttaaacgcg ttgatgatgc
     6361 tttgtatcgg gcgaaaaatg atggacgcaa ccgcgtgctg gcggcataag ccgcggatgc
     6421 gtctcgagat caacgactgc gcttagcgtg gcgctcccag ttttcttgct tcgcctgcgc
     6481 cgctttacgt agtgcgacgt aacacgcccc gctgccgcca tgatgcggta gcgcggtgca
     6541 atatgcctga acatcatcaa attcggtcag ccagcgcgcc acatagctgc ggacaatatt
     6601 ggcatgtgat ttatcttccc gccctttacc atgaataatc agcacattac gcaaaccatc
     6661 cgccagggct tgttgaatga aactgaacac cattttgcgg cactcttcca ccggctggcg
     6721 caaaagattc aggctcgcct gttgcggata tttaccactg cgcagcttat ccagcacccc
     6781 atgctgcaac ccttcctgcc gaaactccag cggctgactt agcgggatga tgtcgagaaa
     6841 tccggtagtg aggaaattat caagctgcag cgtgtcgata cgctgcggcg cacgttggtt
     6901 acgcgttgga tgccagtgga catctgtagc acgtttcagc ggctggacat cttccatggc
     6961 gtcaagaaac agcgatttgt cgtcaaggtt catgtaacct cctatgcacc ttcacacgtc
     7021 gtgattcaga cgggttaatt gttcatactt actgcatcgt tcccttcaca gattgaaaca
     7081 atactctact tcatacctgt gagtatctgc gcaggcgggg catt
//
LOCUS       DADLSA010000179         7015 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.180, whole genome shotgun sequence.
ACCESSION   DADLSA010000179 DADLSA010000000
VERSION     DADLSA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.180"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            242..1219
                     /locus_tag="JN691_003661"
     CDS             242..1219
                     /locus_tag="JN691_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7244981.1"
                     /translation="MILIPDDEPELSLPAASEEFLPALSGENAPVSPARAYLLSLNSH
                     RSRQTMASFLNIVAVMLGAASLESCSWGSLRRHHVMAVTELLRDTGRATATVNTYLSA
                     LKGVAKEAWMLRLMDVESFQHIRAVRNLRGSRLPSGRALPQGEIRALFAVCEADRSCL
                     GARDAAMLAVILGCGLRRSEVVSLDLRDVVTQDRALRVLGKGNKERLAYVPAGAWQRL
                     QIWIDEIRGETPGPLFTRIRRFGDVTLNRLTDQAVYHILQVRQGQAGITKCSPHDLRR
                     TFATAMLDNGEDLITVKDAMGHASVTTTQQYDRRGEQRLQDARDRLNLI"
     gene            complement(1267..1530)
                     /locus_tag="JN691_003662"
                     /pseudo
     CDS             complement(1267..1530)
                     /locus_tag="JN691_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193081.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
     gene            complement(1565..3316)
                     /locus_tag="JN691_003663"
                     /pseudo
     CDS             complement(1565..3316)
                     /locus_tag="JN691_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
     gene            3320..3712
                     /locus_tag="JN691_003664"
     CDS             3320..3712
                     /locus_tag="JN691_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAY7244982.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            3850..4734
                     /locus_tag="JN691_003665"
     CDS             3850..4734
                     /locus_tag="JN691_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAY7244983.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(4766..5965)
                     /gene="tet(A)"
                     /locus_tag="JN691_003666"
     CDS             complement(4766..5965)
                     /gene="tet(A)"
                     /locus_tag="JN691_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HAY7244984.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            6071..6721
                     /gene="tetR(A)"
                     /locus_tag="JN691_003667"
     CDS             6071..6721
                     /gene="tetR(A)"
                     /locus_tag="JN691_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HAY7244985.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(6753..6995)
                     /locus_tag="JN691_003668"
     CDS             complement(6753..6995)
                     /locus_tag="JN691_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HAY7244986.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLMRWKGGGDVVYMALL"
BASE COUNT         1305 a         2151 c         2217 g         1342 t
ORIGIN      
        1 acgccttcag tagctgagca ggagggacaa ctgatggaga tggaagccac gggagcacct
       61 caaaaccacc atcatacact aaatcagtaa gttggcagca tcaccgtaat cgtacagcta
      121 agaacaagtg cttcaagtct acttattaaa atgtaccttt gaaaaagtag actttctgcg
      181 gtacgctctg acagtaagtt tgcccccgat tttttacgca gcgcgccgga cggagcccga
      241 catgatcctg attcccgatg acgaaccgga gctgagtctg cccgcagcca gtgaggaatt
      301 cctgccggcg ctgtcaggtg agaatgcccc ggtcagcccg gcccgggcct acctgctttc
      361 actcaattcc caccgcagcc ggcagaccat ggcctcgttc ctgaacatcg tcgccgttat
      421 gctcggagct gcctccctgg agtcctgcag ctggggcagc ctgcggcgcc atcacgtgat
      481 ggccgtgacc gaactgctgc gcgacaccgg ccgggccacc gccaccgtca acacctatct
      541 ttcggcactc aagggcgtgg cgaaggaagc ctggatgctc cggctcatgg atgtggagag
      601 cttccagcat atccgggcgg tccgtaacct gcgcggcagt cggctgccca gcggtcgggc
      661 gctgccccag ggggagatcc gcgccctgtt tgccgtctgt gaagccgatc gcagctgcct
      721 cggggcgcgg gatgcagcga tgctggcggt cattctcggc tgcggcctgc gacgatccga
      781 agtagtgagc ctggatttgc gtgacgttgt cactcaggac cgtgcgctta gagtgctggg
      841 taagggaaac aaggagcgac tggcatatgt cccggccggt gcctggcagc ggctgcagat
      901 ctggatcgat gagatccggg gcgagacccc gggcccgctg ttcacacgca tccgtcgttt
      961 cggggatgtg actctaaacc ggctaaccga ccaggcggtg taccatatcc tgcaggtgcg
     1021 ccagggtcag gccggcatca cgaaatgttc tccacacgat ctgaggcgaa ccttcgccac
     1081 cgcgatgctg gataacgggg aggatttaat taccgtgaag gatgcgatgg gccacgcgag
     1141 cgtcaccacc acccagcagt atgaccgtcg cggggagcaa cgcctgcagg acgcgcggga
     1201 tagactcaac cttatttagg gctaagtaaa cacaaacaag ggggctgtac cccctctaat
     1261 tttacattaa cacgatactc gacagacttt cccagtcgta gtcagcaacc atcgcccgat
     1321 ggaggctggg ataaatgctg acagcccgac gtgaagcaaa aggctctcgc cgctggcatg
     1381 gcttaatgtc gcggtcccct caatcactaa cacaatttca gcgctggatg tcattaccgt
     1441 ttgattgttc accgcagtga aaatactgaa atggaaatcc tcaacaggaa taggatagcg
     1501 cgcttctgcg gcgtgctgtt ccggtaacat aggggagccc gcagaattcg gaaaaaatcg
     1561 tacgctaagg ttttccgggc atccgtaagg gccgaaactt cccgtcttcc agtctgcggc
     1621 tctgccgcca gacgtaatcg ccggttaggt tgatgtgctc ccagcccagc ggcgacagga
     1681 attgcagcag ctcgccgtcc accggcttgc cggcctcgac caacccctgg gtggcgcgtt
     1741 ccaggtacac cgtgttccac agcacgatag ccgccgtcac caggttgagg ccgctggccc
     1801 ggtagcgctg ctgctcgaag ctccgatccc tgatttcccc aaggcggttg aagaacaccg
     1861 ccctggccag cgagttgcgc gcctcacctt tgttcaggcc ggcatgcacg cggcggcgca
     1921 gttcaacact ttgcagccag tccaggatga acagcgtgcg ctcgatccgg cccagctcgc
     1981 gcagggccac ggccagtccg ttctggcgcg ggtagctgcc gagcttgcgc agcatcagcg
     2041 aggcggtgac ggtgccctgc ttgatcgagc tggccaggcg caggatgtcg tcccagtggg
     2101 cacgcacgtg cttgatgttc agggtgccgc cgatcagcgg gcgcaacgtc gggtaggctt
     2161 gcacgccctg cggcacgtac agcttggttt cgccgaggtc gcggatgcgc ggcgcgaagc
     2221 ggaagcctag caggtgcatc agggcaaaga cgtgatcggt gaagccggcc gtgtcggtgt
     2281 agtgctcctc gatccgcagg tcggactcgt ggtacagcag gccgtcgagc acataggtgg
     2341 aatcgcggac gccgacattc accacgcggg tgctgaacgg cgcgtactgg tcggagatat
     2401 gggtatagaa cagccgtccc ggctcgctac cgtacttcgg gttgacgtgc ccggtgctct
     2461 cgccccggcc acccgcgcgg aagcgctggc catcggagga tgaggtcgtg ccgtcgcccc
     2521 agtgggcggc aaaggcgtgg cgatactggt ggttgaccag ctcggccaag gccgccgaat
     2581 aggtttcgtc gcggatgtgc caggcttgca gccaggacag cttggcgtag gtcaggccgg
     2641 ggctcgactc ggccatcttg gtcagcccga ggttgatcgc atcaccgagg attgcggaca
     2701 gcagcaacgt cctgtctttg gcctcggccc cgtccttcaa gtgggtgaag tggcggctga
     2761 agcccgtcca gtcgtccacg tccatcagca gttcggtgat cttgatgcgc ggcagtaact
     2821 ggctggtttg gtcgatcagc gcctgcgccc gatccggcac cgccgcatcc agcggggtga
     2881 ttttcagccc tgactcggtg aggatggcat cgggcagctc gttgtccttg gccaggcggg
     2941 tgacggtggc caactgctcg tccagcagct gcaaacgctc ttccaggtac tggtcgctgt
     3001 tcgggttgat cgccaggggc agggcctgct cgcgcttgag tgcggcgaac ttctcggccg
     3061 gcagcaggta gtcgtcgaag tcgcggaact gccgcgagcc cttgacccag atgtcgccgg
     3121 agcgcagggc gttcttcagc tcggacaggg cgcagatttc gtagaatttc cggtcgaggc
     3181 cttccggggt gatcaccagc ggcttccagc gcggcttgat gaaggccgtg ggtgcatcgg
     3241 ccggcacctt gcgcaggttg tcggcgttca tctcacgcag ggtctgcacg gctgccagca
     3301 cgccttgcgc ggccggcccg tgctgttccc caacttttcg ctcggcgaag agtatgaaca
     3361 tgcgccgccg gcgacgaatc gccaaatctc accgtatctc ccgagcggac gatttcgcac
     3421 cggtctgccc gtcgaagggc ttgcgatcga acggggcgac cttttctatg catgtccgcg
     3481 agccagcgtc ttttatggca cggcgctcga cgccgacctt cggacgcgcg gcgtaagcac
     3541 gcttgtcatg gccgggataa gcaccaccgg cgttgttctt tcaagcgtcg cctgggctag
     3601 tgatgcggac tacgacgtgc gtttggtcca ggactgctgc tacgacccgg atcgggatgc
     3661 ccacgaagct ctgttgcgtt ccgggttcgg cggacgtgta caggtcgtgt aattttcggg
     3721 cctcgcatga tcgcggccat agccgcaggt ggattggtgc gaggcagggc cagtccaagt
     3781 cagggtgcgg tcatcgcggc gcctgcaact ctcaaaaacc catcgccgta gcggaggtct
     3841 tgtaatcgca tgtcattacg cacgcccgat ttattgttca cagcgatagc acctgccatt
     3901 tggggcagca cctacattgt caccacccaa tacctgccga acttctcacc gatgacggtc
     3961 gcgatgctgc gggcgttgcc ggcgggttta ttgctcgtga tgatcgtccg acagattcca
     4021 acgggaatct ggtggatgcg catcttcatc ctcggcgcac ttaatatttc gctattctgg
     4081 agcttgttgt ttatttcggt ctaccgcctg ccgggcgggg tcgcggcgac ggtaggcgct
     4141 gtgcagccgc tgatggtcgt gttcatctct gccgctctgc taggtagccc gatacgattg
     4201 atggcggtcc tgggggctat ttgcggaact gcgggcgtgg cgctgttggt gttgacacca
     4261 aacgcagcgc tagatcctgt cggcgtcgca gcgggcctgg cgggggcggt ttccatggcg
     4321 ttcggaaccg tgctgacccg caagtggcaa cctcccgtgc ctctgctcac ctttaccgcc
     4381 tggcaactgg cggccggagg acttctgctc gttccagtag ctttagtgtt tgatccgcca
     4441 atcccgatgc ctacaggaac caatgttctc ggcctggcgt ggctcggcct gatcggagcg
     4501 ggtttaacct acttcctttg gttccggggg atctcgcgac tcgaacctac agttgtttcc
     4561 ttactgggct ttctcagccc ggggaccgcc gtgttgctag gatggttgtt cttggatcag
     4621 acgctgagtg cgcttcaaat catcggcgtc ctgctcgtga tcgggagtat ctggctgggc
     4681 caacgttcca accgcactcc tagggcgcgt atagcttgcc ggaagtcgcc ttgacccgca
     4741 tggcataggc ctatcgtttc cacgatcagc gatcggctcg ttgccctgcg ccgctccaaa
     4801 gcccgcgacg cagcgccggc aggcagagca agtagagggc agcgcctgca atccatgccc
     4861 acccgttcca cgttgttata gaagccgcat agatcgccgt gaagaggagg ggtccgacga
     4921 tcgaggtcag gctggtgagc gccgccagtg agccttgcag ctgcccctga cgttcctcat
     4981 ccacctgcct ggacaacatt gcttgcagcg ccggcattcc gatgccaccc gaagcaagca
     5041 ggaccatgat cgggaacgcc atccatcccc gtgtcgcgaa ggcaagcagg atgtagcctg
     5101 tgccgtcggc aatcattccg agcatgagtg cccgcctttc gccgagccgg gcggctacag
     5161 ggccggtgat cattgcctgg gcgagtgaat gcagaatgcc aaatgcggca agcgaaatgc
     5221 cgatcgtggt cgcgtcccag tgaaagcgat cctcgccgaa aatgacccaa agcgcggccg
     5281 gcacctgtcc gacaagttgc atgatgaaga agaccgccat cagggcggcg acgacggtca
     5341 tgccccgggc ccaccggaac gaagcgagcg ggttgagagc ctcccggcgt aacggccggc
     5401 gttcgccttt gtgcgactcc ggcaaaagga aacagcccgt caggaaattg aggccgttca
     5461 aggctgccgc ggcgaagaac ggagcgtggg gggagaaacc gcccatcagc ccaccgagca
     5521 caggtcccgc gaccatcccg aacccgaaac aggcgctcat gaagccgaag tgccgcgcgc
     5581 gctcatcgcc atcagtgata tcggcaatat aagcgccggc taccgcccca gtcgccccgg
     5641 tgatgccggc cacgatccgc ccgatataga gaacccaaag gaaaggcgcc gtcgccatga
     5701 tggcgtagtc gacagcagcg ccggccagcg agacgagcaa gaccggccgc cgcccgaaac
     5761 gatccgacag cgcgcccagc acaggtgcgc aggcaaattg catcaacgca tacagcgcca
     5821 gcagaatgcc atagtgggcg gtgacgtcgt tcgagtgaac cagatcgcgc aggaggcccg
     5881 gcagcaccgg cataatcagg ccgatgccga cagcgtcgag cgcgacagtg ctcagaatta
     5941 cgatcagggg tctgttgggt ttcacgtctg gcctccggac cagcctccgc tggtccgatt
     6001 gaacgcgcgg attctttatc actgataagt tggtggacat attatgttta tcagtgataa
     6061 agtgtcaagc atgacaaagt tgcagccgaa tacagtgatc cgtgccgccc tggacctgtt
     6121 gaacgaggtc ggcgtagacg gtctgacgac acgcaaactg gcggaacggt tgggggttca
     6181 gcagccggcg ctttactggc acttcaggaa caagcgggcg ctgctcgacg cactggccga
     6241 agccatgctg gcggagaatc atacgcattc ggtgccgaga gccgacgacg actggcgctc
     6301 atttctgatc gggaatgccc gcagcttcag gcaggcgctg ctcgcctacc gcgatggcgc
     6361 gcgcatccat gccggcacgc gaccgggcgc accgcagatg gaaacggccg acgcgcagct
     6421 tcgcttcctc tgcgaggcgg gtttttcggc cggggacgcc gtcaatgcgc tgatgacaat
     6481 cagctacttc actgttgggg ccgtgcttga ggagcaggcc ggcgacagcg atgccggcga
     6541 gcgcggcggc accgttgaac aggctccgct ctcgccgctg ttgcgggccg cgatagacgc
     6601 cttcgacgaa gccggtccgg acgcagcgtt cgagcaggga ctcgcggtga ttgtcgatgg
     6661 attggcgaaa aggaggctcg ttgtcaggaa cgttgaagga ccgagaaagg gtgacgattg
     6721 atcaggaccg ctgccggagc gcaacccact cactacagca gagccatgta gacaacatcc
     6781 cctccccctt tccaccgcat cagacgcccg tagcagcccg ctacgggctt tttcatgccc
     6841 tgccctagcg tccaagcctc acggccgcgc tcggcctctc tggcggcctt ctggcgctcc
     6901 tgctgcggcg tccgctcgtg ggccgtggcg cgggtccgcg cgccggcctc gtgcgcctgg
     6961 cgctcgcggg cgaggtccag ggcggccgtc ttcacgttct gccttgcgca gatga
//
LOCUS       DADLSA010000180         6964 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.181, whole genome shotgun sequence.
ACCESSION   DADLSA010000180 DADLSA010000000
VERSION     DADLSA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.181"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..748)
                     /locus_tag="JN691_003669"
     CDS             complement(<1..748)
                     /locus_tag="JN691_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HAY7244987.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLM"
     gene            complement(835..1196)
                     /locus_tag="JN691_003670"
                     /pseudo
     CDS             complement(835..1196)
                     /locus_tag="JN691_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1536..2090)
                     /locus_tag="JN691_003671"
                     /pseudo
     CDS             complement(1536..2090)
                     /locus_tag="JN691_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2233..2355
                     /locus_tag="JN691_003672"
                     /pseudo
     CDS             2233..2355
                     /locus_tag="JN691_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2617..3183)
                     /locus_tag="JN691_003673"
     CDS             complement(2617..3183)
                     /locus_tag="JN691_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244988.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(3465..3779)
                     /locus_tag="JN691_003674"
     CDS             complement(3465..3779)
                     /locus_tag="JN691_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244989.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3857..3967)
                     /locus_tag="JN691_003675"
                     /pseudo
     CDS             complement(3857..3967)
                     /locus_tag="JN691_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4291..4968)
                     /gene="ospD1"
                     /locus_tag="JN691_003676"
     CDS             complement(4291..4968)
                     /gene="ospD1"
                     /locus_tag="JN691_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspD1"
                     /protein_id="HAY7244990.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMISNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(5339..5476)
                     /locus_tag="JN691_003677"
                     /pseudo
     CDS             complement(5339..5476)
                     /locus_tag="JN691_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5558..5856)
                     /locus_tag="JN691_003678"
                     /pseudo
     CDS             complement(5558..5856)
                     /locus_tag="JN691_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..6604)
                     /locus_tag="JN691_003679"
                     /pseudo
     CDS             complement(6445..6604)
                     /locus_tag="JN691_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6608..6964)
                     /locus_tag="JN691_003680"
                     /pseudo
     CDS             complement(6608..6964)
                     /locus_tag="JN691_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1852 a         1565 c         1461 g         2086 t
ORIGIN      
        1 tcattagtgg gatgtaaaaa ctgttacggg tgtactgcac catccgttcc accttacctt
       61 tagtctgtgc cctgaagggg cgacacagtc ggggagagaa gcccatctcc ttgccgaact
      121 gccacagcga aggatggaac cggtgctgac cggtctgata tgcgtcacgt tgcagaacca
      181 cagttttcat attgtcatac aacacttcgc gcggcacacc accaaagaag cggaacgcat
      241 tacgatggca ggtctccagc gtgtcataac gcatattgtc agtgaattcg atgtacaaca
      301 ttcggctgta tccgggaaca gcaacgaaca cgtgaagcgg tgagcgacca ttacgcatag
      361 tgccccagtc aacctgcatc tgtcgtccgg gttcagtttc gaaccgaacg gcaggctcct
      421 gctcctgagg aaccgagaga gaacgaatga atgccctgag aatggtcatt ccgccacgat
      481 atccctggtc tctgatctcg cgagcgatta ccgttgccgg gattttgtaa ggatgagcat
      541 cggcgatgcg ttgacgaata taatcccggt attcatccag gagtgaagca acagcaggtc
      601 gcggcgtata ttttggcggc tcagattttg cctgcaaata acgtttaacg gtattgcggg
      661 agagccccag ttctctggca atcgcccggc tactcattcc ctgcttgtgc aggattttaa
      721 tttccataac tgtctcaaaa gtgaccataa gctctcctga atcaggagag cagattaccc
      781 cctggatctg atttcaggcg ttgggtgtgg atcactattg caccgttcgt gacagtgttg
      841 ttgcgccttg ctgccaacga agctccactg cccatcaagt tcacagataa gggcaacatc
      901 agcgtgagcg actggcgcag atgttatccg ccggggcggg agctttttaa tgtccgtatg
      961 acggtattga tgccgatttt cagcgtacgg gcagtatcgc gaactccggc accgttgaaa
     1021 gccatttcag tgatttggtt tttaactccc ggtttacggg cctcgtaaca gtaagtgagc
     1081 tgaaacaccc ggtgacagtc acggcaacgg aatcgatcat gtccttttgg gttctgcccg
     1141 tgacggtaaa cctgaacgga ctggcaacgg gggcaatgaa agttaactgt gaccatgaaa
     1201 gaaccttaaa agcgggtatt ataaccccta attcaactaa ttggaggcat tgtcattttt
     1261 aatctatgga tacacttaaa aatcctctca ccttacgtta aaaaccttct catgtttaga
     1321 gttttttatt atgtttatca taatgatatg acgatctgag tttttaaaat caagaagaaa
     1381 agataagttt tttaaatgca cagtgcctgt ggataacttt gcgtaaatcc ggaggacaga
     1441 tcactctgaa gcaagacaac agcatctgaa aacaatatgc tcacgtttca accacagggc
     1501 gtaagtgatc ctacccacgt aatatggaca caggcctaag cgaggttctg gttttcaaat
     1561 tgttccggac tgaggccgcc acaccaactg tgccgtcgcc agcgattgta atcacattcg
     1621 atataattaa acactgttgc ccgcattatt tcccggctga taaagtgttc tccgtggaga
     1681 cattccactt tcagcgaatg aaagaagctt tccacgcagg cattatcgta gcagcagccc
     1741 tttgcgctca tactgccacg cagattatgc cacttcagca gcgcctgata atctgctgaa
     1801 cagtactgac ttcctcggtc cgagtgaacg ataacgttct ggggtctctt acgccgccac
     1861 agcgccatct gcagggcatc gcaggccagt tgcgccgtca ggcgtggtga catggaccag
     1921 ccaataacgg cacgtgacca caggtcaatg accacagcca gatacagcta gccttcatct
     1981 gtacgtaagt acgtgatgtc tcccgcccac ttctggttcg gaccactggc gtaaaaatcc
     2041 tgctccagca gattttctga cacaggcagg ccatgtgcgc ggtaactgac cgtattgctg
     2101 ggtaagatca atttgctcac tggtatttac tacgtgcatc gaatgagtga tcaagagccg
     2161 tatgacgcga gagtgtcacg tacggttccg tgagcagccg gagagtgaaa ctctctccgg
     2221 ctgactcgac tcgcaggttc cgacagcggt gacgaacatg cagcggtgtt gttctcgctg
     2281 atcggcacat gccgtctgaa caatgtggag ccagaaaaat ggctgcgtta cgttattgaa
     2341 catatccagg actgaccggc aaaccgggta cgcgatctgt tgccttggaa agttgatctg
     2401 acttctcagt aaatatcaat atgattctga cgagccgctt acctttgagc agagaaaggg
     2461 gagagtgatc ccacatccat gagctaggaa aatacaaaaa ctggctatac aacaattcaa
     2521 tatattagga cttacttgcg aggagaaata tagcaccgct caggcaattc ctgggtattt
     2581 acttttctta gtttctttac tcgggatgtt ctgtcatcag aaaggcgatt ctaaatttgt
     2641 aatatagtca ctcatattcc cacgtaattt ctttagatca atatattgca catttttctc
     2701 aaaaaactga tcggaaataa gatttgcaac attgcttgtt gtatcatttt taacgcttct
     2761 attcattcct tcacaaagtt cgtccatttt ctttttgaaa taactgcttc ctgatacaag
     2821 atgatttatg gaactttgtg cagcattgat gtctagagaa atgcgataat ctttctcaac
     2881 ctgtttaaat atctgtttac ggagattatc gccgattttt cggctgtgtt ctgcacaaaa
     2941 cttatcaata cattggttta ccacccggga tacgtagact tttttttcat catttttcca
     3001 atctgtaata ttttcaccga tttgttctga aacagagctc aaaatactag tatttatttt
     3061 tgttgatgat atttttacta tccttttccc cattgtacag cggataatct catccacctt
     3121 tcctgaaaaa tatcttttgt gagataaact gattccaaaa ttattaaaag atgttccaag
     3181 cattataatc actttgtcat gtaaaatagt aacattgtca ttattttagc tgattcaaac
     3241 aattctgtaa atgctttgag ttctttactc ccttggctca atgatggacc gtctggaatc
     3301 ccagagacta aactctctga gaaagagagt atgaaaaaac ttggggcagt ccctaaatga
     3361 agggaccaac agcggtaaaa taaccggcat cattcataat gatgccctgt tagttgtagt
     3421 tatgaagatt tacgtcatta aaattgctgt tcgtagtgtc agtcctatgg tctggcatca
     3481 gctgaggatc gtagccgata catcactggc ttcacgacat tcgtgttgag gctattcatg
     3541 aaaactccac gctgaaaacg ccgttttgta taaacggtca cggtatgccc ggagccactg
     3601 ttgctgatga attcgataaa accttggcgt ttcttgaagc tatcgttaat gcggataatg
     3661 aaacaacgat tggtgagatt cgctcttttg ccgacgccct ggacgctgtc cggttcaatc
     3721 gcaacaaaat caaccggcag ctgagcaaac ccaaccttgc ctctctggcg ctggagcatg
     3781 aagtgatttg gctgggacgt agccgctgac agcttctata aaagtatgat cctacccacg
     3841 taatatgaac acaggcttaa gtgaggttct ggttttcaaa ttgttcagga ctgagacctc
     3901 cacaccaact gtgccgtcgt cagcgattgt aatcgcactt gatataatta aacactgtat
     3961 atttcatttg tggagtctcc agtctgaggg ggctttatgc attccgtatt gggcactatg
     4021 atgccagaaa tctgcaaggc tccacacctt cctgtatttc ccttcctcta tcacagtgca
     4081 ctttacttaa tagggtggga tgtgttcatt acatttattg tgttttttat gattgttttt
     4141 gcgtccagtg tcgttcgtca tgggaaactg gttctatggg tagtatcgct cagtctagtt
     4201 aactgtttgt ttctttttag aaattaagca gatcacacaa ttcggggaga gatccttcta
     4261 agtgcaattc tttgcagcta tttagcacac ttaaaacaac tcttgtattt tgtcagaaat
     4321 aatattaaac atgtcttgtt cattattgtt aagtctgtct gctatttctg taagttttat
     4381 cccactatga tggcatatat ttatatatgc catcatagcc tcgatattcc ctttccctgc
     4441 ggccagaaat aatcctggta acccctcatt atctcttaca tccaatagtt taatcgtttc
     4501 ttttggtgat aaatcaaaag ttttaataaa atctccataa gcttgaatcg cagcggaatg
     4561 accttcttga agagctatga acaaagcaga ttccccagca ttatttcgtg ctgcaagaac
     4621 ttctagtttt tcctctttgg gtatttcggc tgccaacagc gattcaccgt atttacgcgc
     4681 tgcatcggca tttccatgcc aaatcaatga aaacaaatta tggcggttat actccttatt
     4741 attctttgtc ggaatggata tgctctgtat tttggccaat tttctgaaag aaacaccact
     4801 atatcccccc cctgattttt cttcattgat ggcgtgggat atagcggtat ttgtcacgtt
     4861 aatgatatta cttatcattc ctttattttc attagcagta ttgctgccta ttattaggtg
     4921 catatttttg ttgtttgctg gatgtaatcc atagttattt attgacattt ttgttcatcc
     4981 tttttgctaa aaaaaacaat aacctatttt tgtaatgtaa tagatattag tctattgcta
     5041 atctaaaaaa accattgcca cattgaatgg ctatattcac caataaattc tttcgatttc
     5101 gtatattgac atatcgtaga tatatcttat ccctaagatt caataatcga gtccgtttac
     5161 cattctgtgc aagcgccttc actcagaagt gaccgtcaag gcgatcacca aacttgataa
     5221 taaagcagct cattgccata cgccagtcct taaggggcgt tgtccatttc tgtgatgctg
     5281 cctagattta actgccagct ccggcttatc tacatctaaa tatgtgagcc taataccgct
     5341 acactttggc cagactatgt ttttctccgg gaagtgggct aaccgtctcc ataaagtgac
     5401 gaactttttt caacagtggc catattgaac ggcactgatg attacgggtt attgtctcat
     5461 gaagagccgg ccacatcact gacagcagtg acttcaggcc ttcagcacca gacagcctga
     5521 accagttaat ccatcgcccg acagatttag taatctaggg ctggtttttc ttcagctgtt
     5581 gcggttcgaa agtgccatta cgatcacgca gagtacggat ttccagaggt tcatctccag
     5641 tagtgataat cttcgttaag caaccattgc gggaattgct gccagatcta agctgatttt
     5701 tctcgcaccc aagatgatga gtcatattag cattgagtac ggtttcattt tcagcatcca
     5761 atcaaactga ctgagatctt caggattttt gagacttttg gctagttcat tagctaaagc
     5821 ccgcaactta ttagatgtca actgtttttc attcataaat tcaccttcat ttgatatcaa
     5881 attaaatagt tcaacatcag agaattatac aaacttatgt acaggctcct atgatcttcg
     5941 aatcctgaag attatagtga aaataagttt tcacgttgaa atcccacata gttagtatat
     6001 gataaataat catggatgac ctgattaacc tcttggggta gtgcttttat agaagaataa
     6061 atattctact aatttaatac atcacctaaa atagtattgt tttttatggt tggttgaagg
     6121 tcgtggtgat gatgttagtc cttttgagaa tattccagtg ccagatctta cttataataa
     6181 aggggcttgg cagtttggat aaactattgt tcttattcca ctaatacaaa ctttttgtaa
     6241 agtatattaa atatacttca tttcatactg aaatccccgg aagtattcaa tcaaacaatt
     6301 acaccataaa aggaagaggg ggccctaatt aagttgtcaa gcatgtttat gatacctgca
     6361 gggtataaat tttctggctg caagcagaca catataaaac gtctttattg atcctaccca
     6421 cataatatgg acacagtcgt aagtgaggct ctggttttca aattgttccg gactgagacc
     6481 accacaccaa cggtgccgtc gccagcgatt gtaatcgcac tcgatataac taaacactgt
     6541 tgcccgcact atttcccgat ggataatagt gttctccgtg gagacattcc attttcagcg
     6601 aatgaatttt catctgttgc tgtatccagt catacagtga ctgcatcagt ggcgcggctc
     6661 tggcttttct tgccgccaga cgctgttctg ctgaacatcc ccggacttct gcctcgatgg
     6721 catacagttc accgatacgc tgcagggctt ccgtggtgat gtcggttggc actcttgcat
     6781 gcacatcgtg gatttttctc cggacatgag ccatacatgc ggcttccgtt attctgccgg
     6841 attcgtacaa cgcccggtaa ccaccgtaaa catcggcctg aagcactccg ctgtaaccgg
     6901 ccagatgatt ctgtggatgg atacctttcc ggtccggtga gtacgcgaac cagaccgccg
     6961 gagg
//
LOCUS       DADLSA010000181         6912 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.182, whole genome shotgun sequence.
ACCESSION   DADLSA010000181 DADLSA010000000
VERSION     DADLSA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.182"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..207)
                     /locus_tag="JN691_003681"
     CDS             complement(<1..207)
                     /locus_tag="JN691_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244991.1"
                     /translation="MNMLRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQS"
     gene            727..1422
                     /gene="minC"
                     /locus_tag="JN691_003682"
     CDS             727..1422
                     /gene="minC"
                     /locus_tag="JN691_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAY7244992.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1446..2258
                     /gene="minD"
                     /locus_tag="JN691_003683"
     CDS             1446..2258
                     /gene="minD"
                     /locus_tag="JN691_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAY7244993.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2262..2528
                     /gene="minE"
                     /locus_tag="JN691_003684"
     CDS             2262..2528
                     /gene="minE"
                     /locus_tag="JN691_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAY7244994.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2900..3244)
                     /locus_tag="JN691_003685"
                     /pseudo
     CDS             complement(2900..3244)
                     /locus_tag="JN691_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3279..3398)
                     /locus_tag="JN691_003686"
     CDS             complement(3279..3398)
                     /locus_tag="JN691_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY7244995.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3359..3544)
                     /locus_tag="JN691_003687"
     CDS             complement(3359..3544)
                     /locus_tag="JN691_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244996.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3645..3818
                     /locus_tag="JN691_003688"
     CDS             3645..3818
                     /locus_tag="JN691_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7244997.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3880..4164
                     /locus_tag="JN691_003689"
     CDS             3880..4164
                     /locus_tag="JN691_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAY7244998.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            4168..4503
                     /locus_tag="JN691_003690"
     CDS             4168..4503
                     /locus_tag="JN691_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAY7244999.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4560..6881)
                     /locus_tag="JN691_003691"
     CDS             complement(4560..6881)
                     /locus_tag="JN691_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7245000.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
BASE COUNT         1868 a         1641 c         1567 g         1836 t
ORIGIN      
        1 tgattgtaag ttttcagcgg ctttttcgcc gagatatttc cttgttaact ctggggaaat
       61 gccatctgag ttcacacaaa tatacgggtc gcaaacaacg ccgcacgccg gagattgaag
      121 tccgccagcg tcatgtccag acgccattga tgagaacatc atccccacac ctgacaatcc
      181 gatataaata atacgcaaca tattcatagc acatcctgat aatccaaccg tacaacactt
      241 atgctcattt cgtcatcaat ccgtaatcaa aaccgctgac ctggacgagt tacgatgacc
      301 tgacagaagt atcagacatc ttctgcggtt tctatgatag agcaattcta tggcggatat
      361 taagataacg ggtatctgtt ctttaatgat gctgcaaaaa atggaaagtt aagaaagcct
      421 ggcgtggtga aaaaatacac ttgcctcaat actggatatt taaacaggtc tggagatgct
      481 gtcacgatta gggtgcggaa aaaggaagcg taaataagaa gaatcaaaag tacgacggca
      541 atgggttgat tgacaagggt attttttaag ctatgaatca gcgccattta tcacagaata
      601 gacttttact ctgaataaat gggagggtga cttgcctcaa tataatccag actataacat
      661 gccttatagt cttcggaaca tcatcgcgcg ctggcgatga ttaatagcta attgagtaag
      721 gccaggatgt caaacacgcc aatcgagctt aaaggcagta gcttcacttt atctgtggtt
      781 catctgcatg aggcagaacc taaggttatc catcaggcgc tggaagacaa aatcgctcag
      841 gcccccacat ttttaaaaca tgcccccgtt gtactcaacg tcagtgcact ggaagacccg
      901 gtaaactggt cagcgatgca taaggcggtt tcggcaaccg gtttgcgggt tattggcgta
      961 agtggctgca aagatgcgca acttaaagcc gaaattgaaa aaatggggct gcctatcctg
     1021 acggaaggaa aagaaaaagc gccacgtcca gctcccgcac cgcaggctcc agcgcaaaat
     1081 acaatgccgg tcacaaaaac gcgtttaata gataccccgg tgcgttccgg tcagcgtatt
     1141 tatgctccac aatgtgatct gattgttaca agccacgtta gcgctggggc cgaattgatt
     1201 gccgatggga acattcatgt ctatggcatg atgcgcggtc gtgcgctggc aggggcaagt
     1261 ggtgaccggg aaacgcaaat attttgtacg aacctgatgg cggaattggt gtccatcgca
     1321 ggtgaatact ggctgagtga tcaaatccca gcagaatttt atggcaaagc ggcgcgactg
     1381 cagttagtcg aaaacgcttt gaccgttcaa ccgttaaatt gatccctttt tgacaaggaa
     1441 tttctatggc acgcattatt gttgttactt cgggcaaagg gggtgttggt aagacaacct
     1501 ccagcgcggc catcgccact ggtttggccc agaagggaaa gaaaactgtc gtgatagatt
     1561 ttgatatcgg cctgcgtaat ctcgacctga ttatgggttg cgaacgccgg gtcgtttacg
     1621 atttcgtcaa cgtcattcag ggcgatgcaa cgctaaatca ggcgttaatt aaagataagc
     1681 gtactgaaaa tctctatatt cttccggcat cgcaaacacg cgataaagat gccctcaccc
     1741 gtgaaggggt cgccaaagtt cttgatgatc tgaaagcgat ggattttgaa tttatcgttt
     1801 gtgactcccc ggcagggatt gaaaccggtg cgttaatggc actctatttt gcagacgaag
     1861 ccattattac caccaacccg gaagtctcct cagtacgcga ctctgaccgt attttaggca
     1921 ttctggcgtc aaaatcacgc cgcgcagaaa atggcgaaga gcctattaaa gagcacctgc
     1981 tgttaacgcg ctataaccca ggccgcgtaa gcagaggtga catgctgagc atggaagatg
     2041 tgctggagat cctgcgcatc aaactcgtcg gcgtgatccc agaggatcaa tcagtattgc
     2101 gcgcctctaa ccagggtgaa ccggtcattc tcgacattaa cgccgatgcg ggtaaagcct
     2161 acgcagatac cgtagaacgt ctgttgggag aagaacgtcc tttccgcttc attgaagaag
     2221 agaagaaagg cttcctcaaa cgcttgttcg gaggataagt tatggcatta ctcgatttct
     2281 ttctctcgcg gaagaagaac acagccaaca ttgcaaaaga acggctgcag attattgttg
     2341 ctgaacgccg tcgcagcgat gcagaaccgc attatctgcc gcagttgcgt aaagatattc
     2401 ttgaggtcat ttgtaaatac gtacaaattg atcctgagat ggtaaccgta cagcttgagc
     2461 aaaaagatgg cgatatttct attcttgagc tgaacgtgac cttaccggaa gcagaagagc
     2521 tgaaataagc ccgctgtaaa aacgcattta tcttcaaggc agaatcattt ctgccttgag
     2581 tttttcatcc ctctcatcca cgttgtggta aagcggcgag tattcttgct gatactcctc
     2641 attgctattt ccacctcccc ctttacacct taaggctgtt cccacgcaca gaacaaaatg
     2701 tttatgcctg gttgagtaaa taaccatgtt gttagtgtgg atatactggg agatgataac
     2761 cccgcaattt cattattcat tgttctccca taggatgagg tttcccgccg taactggcgg
     2821 gctttttttg ccccagaatt ttctctttca acatcctgta aacgaaaact gcgccgaagc
     2881 gcagtttaat cgttgatggt cagaacgacc agttcacacc agccaccgcg ttccacgggg
     2941 actccacacc ggcaccatgg ctatacccca ccccgaaatg cccgcttaac gtactgctga
     3001 atgaggcttt aatacctgcc tggtatattc cacgtctgcc cgacaaatca ttgacaaaat
     3061 taccgtcact attcactttc acccgattat catcgacaaa ttctttgcgc acagccgcct
     3121 tcaggcaggg ttcaatttcc ataccgttcc ccagacgcat gttgtaactc agcgttgcac
     3181 ccagttcacg atatatactg cgggtatcga ctgatttcga ttccatgcca ttggataaat
     3241 gatagtagcg tctaagtgac ctaagctctg ctgcaacatc agcccgcagg caccagacca
     3301 ggggattcat cctgaagaga cagcgcaggt gtattgtgtt caccgctcac caaagacatc
     3361 atgatgaaat gatgatatcc cgcataagaa agaggcattt tttacacgca gtgcgctgaa
     3421 gtgtagttgg ataaaaaatc actcttttca gataatactg tcgtacgctt atctttagac
     3481 aatgaggccg tcggcaaaat aaaatgacta atgtaatcgt tgctgatgaa tatcttctgc
     3541 tcacataaaa atcacacaat aatattgata aatcagatta tcttaccttt acaacattgc
     3601 gttaacagtc aatgacactt cttgaataaa aatattgtca atcaatgagt tattcctcat
     3661 ttaagattat tctcattcac gtaaaaaaca taattcctat catcacagcc acgctcattt
     3721 tgaattatct gaataatagc gagcgttctt tagttaagca gatcttaatt gaagacgaaa
     3781 ttattgtgtg tgcaacttac ttaataccgg acatctaagt tgaagaaaaa aatacttgcc
     3841 ttcgggctta tctcagcatt gttctgctcc acgccagcga tggcggatat gaaccgcacc
     3901 acgaaaggtg cattgctagg cgcaggcgtt ggtttactta ctggcaatgg tgttaatggc
     3961 gtactgaaag gtgcagctgt tggcgctggt gttggtgcag taacagaaaa aggccgcgac
     4021 ggtaaaaatg cgcgtaaagg tgcaaaagtg ggtgccgccg ttggtgctgt cactggcgtt
     4081 ctgaccggca atggcctcga aggggcgatt aaaggtgccg tgattggcgg tacgggtggt
     4141 gcaattctgg gtaaaatgaa ataaggaatg atgatgaaaa aatttgcttt actggcgggc
     4201 ctgtttgttt tcgcaccaat gacctgggcg caggactaca atattaaaaa cggtttgccg
     4261 tcagaaacct atattacttg cgcagaagcc aacgaaatgg caaaaactga cagcgcacag
     4321 gttgcagaaa ttgttgcggt aatgggtaat gccagcgttg ccagccgtga tttaaaaatt
     4381 gagcaatctc cggaactgag cgccaaagtg gtagaaaaac tgaatcaggt ttgcgctaaa
     4441 gacccgcaaa tgctgttgat cacggcaatt gatgacacca tgcgtgctat cggcaaaaaa
     4501 taatatttca tatgtaccgc gtcgatgaat acgacgcggt tttttagcta acggcgggat
     4561 cagaacttat atttcaggcc caccatcact gcggtgtcat tgtagccatt atcacccagc
     4621 tgcacgccca cattccccca gacgctggcc gctggattca ggttgccatt tacgccaagt
     4681 ttaatttccc ccaaattact caccccatct tgttcgactt tcacaccgtt cattgagaca
     4741 gcaaaatctt tactgttatg gatccagttc atttcaacaa aaggttcaaa gtcgtcaccg
     4801 tgcggaccgc tgtttttctc ctgagtacga ataaaggtgc ggaagccgag tcgggtctgg
     4861 atgttgttat ttgcgtcatt ctcaatacgt gagccattgg cttccgtgtg cgcattatga
     4921 tcgacgccca tccagatagc ctgtgcctgt ggctggaaga tccacgtata tttcgccgct
     4981 tccagaccaa aggatttatt cagggcaaaa ccgtaacccg cttccagcga gacggttgca
     5041 cctttcgcgc tataggattc ataggctaat tcatcccctt tcacggagtt tttaaaccag
     5101 ctatattgcg cccaggagtc aatgtatgcg ccttttttac tgatgtcatc ggcaaaccag
     5161 gtggcataca gccctgcgct atagcctctg acactccctt tcgaacgata atccgacacg
     5221 ctggaatgag ttaagttgta gtcgcgggca taaccagcca tcactcccaa acgccaactg
     5281 tcactatcgg taaaaccacc ggttaacaga tcgcccccta actgcgagac gtagcgatgc
     5341 gaggtcgttc tcaactgtcc gttgctgtca cgccaggcat tatgcccgcc aatttgacgt
     5401 agccaaagtc ggcttgaacg ctcctgttca gttacaggat cgatgtagcg cgtttcaccc
     5461 gcacggtcgt tcagacgcat aacaaaaaga gtgttagcgg ctgccaggtt ggcgacgtaa
     5521 cttccagctt cggggcgcag gacgggggta ggcgcaacag taggcgctga acttcctccg
     5581 gagtttgttt caccgccgct cgttgcctgg aagttagtca gataccagtt tttattggtc
     5641 gcttcggtat tacctcgggt taatgaatat tcgtaggcac cggcgaaaat cctcgaatcc
     5701 ttaataaatt ccccatttga ttccccctca acgctgataa tctctatccc atttaacgtg
     5761 taagctccgc tgcctcctgc attggtaact ttaactcggg ttgttccaga ggtatttcca
     5821 ttaataacga ctttatccgt agctgaatta tcatcatcca attcagttct gagatgcaat
     5881 acgccattat taccaacgta attttcagtt attgttaatc ttgttggttc gacatcccgt
     5941 ccatctgccc gggaaatata gaccgtacta ttatcaaccg tgaggttgct caccgttgag
     6001 tcagctttaa tatcccaggt actattatct gaaagcgaca gataggtatg agtgttatca
     6061 tctgttgaaa tattagctga acccgttatt tttgaattag ttgcatcgac ggtcaaatga
     6121 cctttaatat tattgataaa aacatctgca gccgatgata cctcactatc cgtaatggtc
     6181 acgttactcg tctcattttt atggtagagg atttcatttg ctgaccgtag tgagctacca
     6241 gataaaatat ctaccgttgc ttcctgacca tttatggaag actccagcac aattccagac
     6301 ccctgatgcg cgactgcaga tgtatttttc agcgtcaccg tgccgccatt ataaatttta
     6361 gcaatcgcgg cgtcaggacc cgttgtataa atttctccct ggttaaatga aactgtcccc
     6421 tggcgggcag cgatggcatg agcattgtcc ccatgcgtgg tgatatcaac atcggttaat
     6481 gtaacgctgg cattggtata atcaagcgtg tcaccagcct tgacgacagt gccattagcg
     6541 tacgcggcat aactttctgt tccgtgggtt gtgatctccc cttttgccat atccagtttt
     6601 gctatggcat tattttcatt acttcctgta tcggcagtac tttcttcatc aagagaagat
     6661 acacacgcac gcgcgcttcc gggagatgca cacgcaagaa cgccgactgc gtgattaccc
     6721 gcagtaacaa taaatgtatc atctccgtca gaaaaagagg gggtaaccga accgtcagag
     6781 ttgactctta ttatggtgtc agtattcagg tttccactcg tttgacttag caaaccaatt
     6841 gagtaatccc ccgttgtgac aatcgtgttt ttaccctgca aaataacagc accgccaaat
     6901 tcacttacga tt
//
LOCUS       DADLSA010000182         6887 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.183, whole genome shotgun sequence.
ACCESSION   DADLSA010000182 DADLSA010000000
VERSION     DADLSA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.183"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            8..892
                     /locus_tag="JN691_003692"
     CDS             8..892
                     /locus_tag="JN691_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAY7245001.1"
                     /translation="MLVTLATGRGLMLVGEPGTAKSLLSELLATSISGDAGLTIQGGA
                     STTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITRTPLEVQDCLL
                     GMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKRRFDFETVFPI
                     MDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEKKTSMDTLTAI
                     MSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRARLRRYFEQRVA
                     THKEAHWQAYYQARHRLP"
     gene            903..3182
                     /locus_tag="JN691_003693"
     CDS             903..3182
                     /locus_tag="JN691_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245002.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGNSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSKKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLAPFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            3175..4311
                     /locus_tag="JN691_003694"
     CDS             3175..4311
                     /locus_tag="JN691_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY7245003.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            4308..6311
                     /locus_tag="JN691_003695"
     CDS             4308..6311
                     /locus_tag="JN691_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HAY7245004.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            6436..6887
                     /locus_tag="JN691_003696"
                     /pseudo
     CDS             6436..6887
                     /locus_tag="JN691_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
BASE COUNT         1603 a         1824 c         1879 g         1581 t
ORIGIN      
        1 cgaacgtatg ttagtcactc ttgccaccgg acgcggtttg atgttggtgg gggaacctgg
       61 caccgcaaaa tctctcctgt ctgaattact ggcaacctct attagcggcg atgccgggtt
      121 aaccatccag ggcggcgcat ctactaccga agatcaaatc aagtacggct ggaactacgc
      181 cctgcttatc aatcatggtc catcaacgga agcgctggtc cctgccccgc tttaccaggg
      241 aatgcgcgac ggcaaaatcg tccgttttga agagataacg cgtacaccgc tggaagtgca
      301 agactgtctg ttaggtatgc tttctgacag ggtgatgacg gtgccggaac tcactggtga
      361 agccagtcag ctctatgcgc gagaaggctt taatattatt gccactgcca atacccgcga
      421 tcgcggcgtc aatgaaatga gtgctgcgct aaagcgtcgc ttcgattttg aaactgtctt
      481 tccgattatg gatttcgccc aggagctgga gcttgtcgcc agtgcttcgg cgcgtttgct
      541 ggcccatagc ggtattccgc ataaagtgcc tgacgcggta ctggagttac tggtccgcac
      601 cttccgcgat ctgcgtgcca acggcgaaaa gaaaacgtca atggatacac taacagcgat
      661 tatgtccacc gctgaagccg tgaacgtggc ccacgccgta ggcgtccgcg cctggttttt
      721 agcgaatcgc gcaggtgagc cagccgacct ggtggactgc attgccggaa ccatcgtcaa
      781 agataacgaa gaagatcgcg ctcgtctgcg ccgttacttc gaacagcgtg tcgccacaca
      841 taaagaagct cactggcagg cttattatca agcccgccac cgcctgccgt gaagaaagat
      901 gcatgagcga gccgttaatt gtcggcatcc ggcatcatag tccggcctgc gcccggctgg
      961 tgaaatcgtt aatcgaaagc cagcggccac gatacgtgtt gattgaaggc ccggctgatt
     1021 ttaatgaccg ggtagacgaa ctgtttttag cccaccagct tccggtagct atttacagtt
     1081 attgccagta tcaggacggt gcagcccccg ggcgtggtgc ctggacgcca tttgctgaat
     1141 tttcgccgga gtggcaggcg ctacaagccg atcgtcgtat tcaggcacaa acttacttca
     1201 tcgatttgcc ttgctgggcg cagagtgaag aagaggacga ttcgcctgat acgcaagagg
     1261 aaagccagac cttgctgctg cgtgccaccc gcatggataa cagcgacacc ctgtgggatc
     1321 acttgttcga agatgaaagc cagcaaactg cattaccctc tgcactggcg cactattttg
     1381 cccaactgcg gggcaactcc cccggcgatg ctctcaatcg tcagcgcgaa gcctttatgg
     1441 cccgctggat tggatgggcg atgcagcaaa ataatggcga cgtgttagtt gtctgcggtg
     1501 gctggcacgc tccggcactg gcaaaaatgt ggcgcgaatg tccgcaggta attaacacgc
     1561 cagaattgcc gtcgctggca gatgccgtta caggttgtta tctcaccccc tacagtaaaa
     1621 agcgccttga tgtgctggca ggataccttt caggaatgcc tgccccggtc tggcaaaact
     1681 ggtgctggca gtggggctta cagcaggcgg gagaacaact gctaaaaacg gttctcaccc
     1741 gtttgcgcca gcacaagttg cctgcttcga ccgcggatat ggctgccgct catctgcatg
     1801 cgatggcact ggcacagttg cgcggtcata cactaccgtt acgcactgac tggctggatg
     1861 ccatagcagg atcgctgatt aaagaagccc tgaatgcgcc gttgccgtgg agctatcgcg
     1921 gcgttattca tcccgatacc gatccgattc tgctaacgtt gatagacaca ttagcgggtg
     1981 acggattcgg taaacttgcc ccttctacgc cacaaccgcc tctgccaaaa gatgtcactt
     2041 gcgaactgga acgtaccgca atctctcttc cggcggagct taccttaaat cgctttaacc
     2101 ccaatggact agcgcaaagt caggtgttac atcggctggc aatactggag atcccaggga
     2161 ttgtacgcca gcagggaagc acactgacac ttgcaggcaa cggtgaagaa cgctggaaat
     2221 taacccgccc gcttagccag catgcggcat tgattgaggc cgcctgcttt ggtgccacac
     2281 tccaggaagc cgcacgccat aaattagaag ccgatatgct ggacgcgggt ggaatcggca
     2341 gcattaccac atgtcttagc caggcggcgt cagcgggtct ggcgcccttc agtcaacaat
     2401 tactggagca actcacatta ttaatcgccc aggaaaatca atttgccgaa atgggccagg
     2461 cgctggaagt gctatatgcc ttatggcggc tggatgaaat tagcggtatg caaggcgcgc
     2521 agatattaca aacgacgtta tgcgcggcta tcgatcgcac gctgtggctg tgtgaatcca
     2581 acggcagacc ggatgaaaag gagtttcacg ctcacttgca tagctggcaa gcgttgtgtc
     2641 atatcctgcg cgatctacat agcggtgtgc aactccccgg cgtttcgctt tctgcggcgg
     2701 tagccttgct ggagcgacac agtcaggcga ttcatgtcct ggcactggat cgcggcgcga
     2761 ctcttggtgc gctgatgcgt ctggaacatc ccaacgctag tgccgaagcg gcgctgacga
     2821 tgctggcgca gttatccccg gcacaatccg gcgaggcgct gcacggtttg ctggcattag
     2881 cccgtcatca actggcctgt cagccggcat ttatcgccgg tttcagcagt catttaaatc
     2941 aactaagtga tgccgatttt accaatgccc tgcccgattt acgcgcggca atggcctggc
     3001 taccgccacg agaacgcggg acgctggcgc atcaggtgct tgagcattat caactggcgc
     3061 aacttcccgt ttcggcactg caaatgccgt tgcattgtcc accgcaagcc attgcacatc
     3121 atcaacaact cgaacagcag gcactggcat cgctgcaaca ctggggagtt ttccatgtct
     3181 gaactgaacg atcttctgac cacccgtgag ctacaacgct ggcgattaat tcttggcgaa
     3241 gcggcagaaa cgacgctttg tgggctggat gacaacgccc ggcagataga tcacgcgctg
     3301 gagtggctgt atgggcgcga tcctgaacgg ctccagcgtg gtgaacgctc tagtggatta
     3361 ggtggctcaa atctcaccac tcctgagtgg atcaacagta ttcacacgct gtttccgcaa
     3421 caggtgattg agcggctgga aagcgatgcc gtactgcgct acggcattga agatgtggtg
     3481 acaaatctcg acgtgctgga acgtatgcag ccttccgaaa gcctgctacg cgccgttttg
     3541 cacaccaaac atctgatgaa ccccgaagta ctggctgccg cccgcaggat tgtgcgccag
     3601 gttgtggaag aaattatggc gcgactggca aaggaagttc gtcaggcttt ttctggtgtc
     3661 cgcgatcgtc gtcgccgctc atttattcca ctggcgcgaa actttgattt caaaagtact
     3721 ctgcgcgcca acctgcaaca ctggcacccg caacacggca agttgtatat cgaattcccc
     3781 cgctttaaca gccgcattaa acgccaaagc gagcaatggc aactggtctt actggttgat
     3841 caaagcggat cgatggtcga ttcggtgatc cactctgcgg tgatggcggc ctgtttatgg
     3901 cagttacccg gcattcgtac ccatctggtg gcgtttgaca caagtgtcgt tgatctcacg
     3961 gcagacgttg ccgatccggt agagttatta atgaaagtgc aattaggcgg cggaaccaat
     4021 atcgccagtg ccatggagta tggtcggcaa cttattgaac aacctgcaaa aagcgtcatt
     4081 atcctcgtga gcgattttta tgaagggggt tcatcatcat tgctgacgca ccaagtgaaa
     4141 aagtgtgtcc agagcggcat caaagtgctg ggactggcag cgctcgatag cacagcaaca
     4201 ccttgctatg accgcgatac ggcccaggcg ctggttaatg tcggcgcaca aatagccgcc
     4261 atgacaccgg gcgagctggc atcatggctt gcggagaatc ttcagtcatg aattcactac
     4321 gtccggaatt attagaactg acaccgcagg cactgacggc gttaagcaat gccggttttg
     4381 ttaagcgcag ccttaaggaa ctggaaaatg gcaacgtccc ggaaatcagc catgagaaca
     4441 gcgctttaat cgccaccttc agtgacggtg tccgtaccca gctggcgaac ggccaggcac
     4501 tgaaagaggc tcagtgcacc tgtggggcca gcggcatgtg ccgtcatcgc gtgatgctgg
     4561 tgttaagtta tcaacgactt tgtgctaccg cccagcctac gggaaaagaa gaggaatggg
     4621 atccggcaat ctggctggaa gaactggcta cccttcccga tgccacccgc aaacgcgcac
     4681 aggcgctggt cgctaaaagc atcaccattg agttgttctg tgcgccgggt gaaattccct
     4741 ctgcccgctt accgatgagc gatgtgcgtt tttattcccg cagcagtatt cgtttcgccc
     4801 gttgtgattg tattgaaggc acactttgcg aacatgtcgt actggcagta caggcctttg
     4861 tccaggcaaa agcgcagcaa gcggaattga ctcatttaat ctggcagatg cgcagcgaac
     4921 acgtcacatc atctgacgat ccgtttgcca gcgaagaagg caaaacgtgt cgtcaatatg
     4981 ttcaacaact aagccaggca ttatggctga gcggcatcag ccagccgctt attcactacg
     5041 aggccgcttt cagccgcgcg cagcaagcag cggaacgctg cagctggcga tgggtgagtg
     5101 aatcgctacg gcaactgcgc gccagcgttg atgccttcca cgcccgcgcc agccactatc
     5161 atgccggaga atgcttacgt cagcttgcgg cattaaacag tcgattaaat tgcgcacaag
     5221 agatggcccg gagcgacagt gttggtgaag ttcctcctgt gccgtggcgc acggtcgttg
     5281 gctctggcat tgccggagaa gcaaagcttg atcatctgcg gctggtgtct ttaggtatgc
     5341 gttgctggca ggatattgag cattatggtt tacgcatctg gtttaccgat cccgacaccg
     5401 gcagtatttt gcacctttcg cgcagttggc cgcgaagtga acaggaagac tcaccggcag
     5461 ctacgcgtcg gctgtttagt tttcaggctg gcgcactggc gggtgggcaa attgtttcac
     5521 aagcagcaaa acgcagtgcc gatggcgagc tgctgttagc tacccgcaac cgcttaagca
     5581 gcgttgtgcc gctgtcgcct gatgcctggc aaatgttgag cgcgccgtta cgccagccgg
     5641 gcattgtggc tttgcgggaa tatttacgcc agcgtccccc cgcctgcata cggcctctta
     5701 atcaggtcga taacttattt attctgccgg tcgctgagtg tatttcgctc ggttgggata
     5761 gcagccgcca gacgctggat gcgcaggtaa tcagcggcga aggtgaagat aatctgctga
     5821 cgttatcact gccagcctca gccagcgcgc cttatgccgt tgaacgcatg gcggcgcttt
     5881 tgcaacaaac agacgacccc gtgtgtctgg tttctggctt tgtcagtttt gttgatgggc
     5941 aattgacact ggaaccacag gtaatgatga caaagactcg tgcctgggcg ctggacgcag
     6001 aaactgcgcc tgtggtggta tcactacctt ctgccagcgt tctgcctgtg ccgtctactg
     6061 ctcatcagtt gctgatgcgc tgccaggcgt tacttattca actgctccat aacggctggc
     6121 gctatcagga acagagcgct attagtcagg cagagttgct ggcgaatgac ctcaccgcgg
     6181 tgggttttta tcggctggca catgtgttgg cacaatttcg taatacagaa agcgaggcac
     6241 gggtagaagc aatgaataac ggtgttttgc tttgcgaaca attattcccc atgcttcagc
     6301 aacaaggatg aaatagtgct ttttactaag agttctactc cagttccgga ctgctcacgc
     6361 cacggtatta ggcatatcct atatagcccc tggtgagagt caccagtttc ttgattaaat
     6421 aaaatggagt tttacatgaa ggctttcaat aagctgtttt ccctcgttgt tgcatctgtt
     6481 ctggttttct ctcttgctgg ctgcggtgac aaagaagaat cgaagaaatt cagcgccaat
     6541 ctgaacggca ctgaaattgc cattacctat gtctacaaag gtgacaaggt gcttaagcaa
     6601 tcttctgaaa ccaaaattca atttgcctcc attggtgcaa ccaccaaaga agatgctgcc
     6661 aagacacttg agccgttaag cgccaaatac aaaaacatcg cgggtgttga agaaaaatca
     6721 acctataccg atacctacgc gcaggaaaac gtgactatcg atatggaaaa agtggatttt
     6781 aaagccctgc agggtatttc atgaatcaac gtttctgctg aagatgccaa aaaaggtatc
     6841 actatggcgc aaatggaact ggtgatgaaa gccgctggtt ttaaaga
//
LOCUS       DADLSA010000183         6884 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.184, whole genome shotgun sequence.
ACCESSION   DADLSA010000183 DADLSA010000000
VERSION     DADLSA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.184"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(102..1316)
                     /gene="rspA"
                     /locus_tag="JN691_003697"
     CDS             complement(102..1316)
                     /gene="rspA"
                     /locus_tag="JN691_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HAY7245005.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1521..1847)
                     /locus_tag="JN691_003698"
     CDS             complement(1521..1847)
                     /locus_tag="JN691_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HAY7245006.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1982..2323
                     /locus_tag="JN691_003699"
     CDS             1982..2323
                     /locus_tag="JN691_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HAY7245007.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2389..2918
                     /gene="speG"
                     /locus_tag="JN691_003700"
                     /pseudo
     CDS             2389..2918
                     /gene="speG"
                     /locus_tag="JN691_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2921..3631)
                     /locus_tag="JN691_003701"
     CDS             complement(2921..3631)
                     /locus_tag="JN691_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAY7245008.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3739..4044
                     /locus_tag="JN691_003702"
     CDS             3739..4044
                     /locus_tag="JN691_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAY7245009.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4243..5700
                     /locus_tag="JN691_003703"
     CDS             4243..5700
                     /locus_tag="JN691_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7245010.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWLETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTTKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     LRDGVRGKDKLDVPIKFIWNFKDLKRTLSRPEVLH"
     gene            complement(6371..>6884)
                     /locus_tag="JN691_003704"
     CDS             complement(6371..>6884)
                     /locus_tag="JN691_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245011.1"
                     /translation="VDVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTEPSFGSE
                     VSDYGIIAHESQLKALSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTA
                     LSFGCFRHDGENITVVLSRDGDSQSHDLEILGKVFVVAEAATTVKMGTGSATAVLPVV
                     KRLKFTKTEA"
BASE COUNT         1829 a         1763 c         1622 g         1670 t
ORIGIN      
        1 cttcgggata acgcttaccg ctcatgggca cctctcttta agccatctta aatgactctg
       61 aggtgtctgt taaacccgtg gcgattcaat tatcttactc cttacctgtt ccacagcgtg
      121 ccatcttcca gacgtgcgac tggtagataa gcaggttcat agggatattt cgccgccagc
      181 ttttcatcga attcgatacc aagacccggt ttgtctcccg gatgcatata gccgttatcg
      241 aaagtccagt tgtgcgggaa gacttcgagc atttgttcgg aataacccat gtattcctgg
      301 acaccgaaat tggggaccca cagatcaaag tgcagcgccg cagccatgca gactggtgac
      361 aaatcggaag gaccgtgtga gccagtacgt acctgataca gcgaagcaaa atcggcaatc
      421 cggcgcatac cggtaattcc gcctgcatgg gtcagcgtgg tgcggatata atcgatgagt
      481 tgctcttcaa tcagttgttt gcagtcccag atgctgttga agacttcacc cactgcgatg
      541 ggtgtgacgg tatgttggcg aatgagacgg aagcattcct ggttttccgc tggcgtcggg
      601 tcttccatcc agaacatgcg ataatcttca atgcttttac caaagcgcgc cgcttcaata
      661 ggcgttaagc gatggtgcat gtcatgcagc aaatgttcat taaaaccaaa cttgttacgt
      721 accgcgtcaa acaatttcgg catgaaatcg aggtatttct ccgtcgacca cagctgctct
      781 tccggccact gtcctttggt tgcgggttca taagccagac ctttaccttt cgacatgccg
      841 taggtggttt tcataccagg gattccgcac tgcacgcgga tggctttgaa tcccagctcc
      901 tgatgacggg cataatcatc cagagcttca tcaatactgt gaccggtggt atggcaataa
      961 accatcaccc cttcacgaga cgcgccgccg agtaactggt aaagcggcat gttggcagct
     1021 ttggctttaa tatcccacag cgccatatca accgctgaaa tggccgacat cgtaaccgga
     1081 ccgcgacgcc agtaagcacc tttatagaaa aactgccaga tatcttcgat acggtgcgca
     1141 tcgcgaccaa taagccgcgg acaaagatga tcctgcaaat aagaggccac ggaaagctca
     1201 cgtccattga gggtggcatc cccaaggccc gtaataccgt cctcagtggt gatttttaat
     1261 gtgacgaaat tacgccccgg acaggtaaca aaaacttcag cctttacgat cttcatgttc
     1321 gattccttgc atcgcttgtc gtgatgcatg aaatctacgc aactgagcca ctgccataca
     1381 agtatgaaga tcgaaaaagc cggagtgatc acaaaaaaag gcgtatattt gcgctgtgaa
     1441 tggttgacaa aagatgaaat agaatacctt ttgtcagctg acacttcctc gtatcttatt
     1501 gataaaatgg atttatgttc ctacgtgcgc ccccagcccg caacaatgat caacattccg
     1561 caaagcgcaa tcaacgcacc cgtccagtca taaagactta gtttcacgcc atccacaacg
     1621 cgcagccaca tcaatgccgt gcagacataa acgccaccat aagccgcgta aacacgccca
     1681 ctcgccgcag gatgcaacgt taacaaccag acaaacagcg ccagtgaaat ccccgccgga
     1741 agcaacagcc agatactggc gtttcgtttt aaccacaacc agggcagaaa gcatccaata
     1801 atttcacaca gcgcagtagc aaaaaatagt aacgttgttt taatcatctt tgtcacttat
     1861 tgacatcatg tatagttata gggcgacata atatcatcaa tataaacatc ctcctggtac
     1921 gttttgcgtt cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat
     1981 tatgaaaatc actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact
     2041 ggcattgagc acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag
     2101 tgcacaaagc cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct
     2161 gcgccagaaa gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga
     2221 taaccgcgat aaatgtgagc aaagcgccaa catcaatgcc tactgggagc ccaatacttt
     2281 gcgctgcctg gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag
     2341 gaatgtaagg acacgttatg ccaagcgccc acagttaagc tacgcccgct ggagcgtgaa
     2401 gatttacgct atgtacatca actcgacaat aacgccagtg tgatgcgtta ctggtttgag
     2461 gaaccctacg aagcctttgt tgaactctct gatctgtatg ataagcgcat attcacgatc
     2521 agagcgaacg gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg
     2581 tggaaattaa ccatgttcat cgccgggcag aatttcagat aattatctcc ccggagtatc
     2641 aggggaaagg tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc
     2701 tcaatctcta taagctgtat ctgatcgtcg ataaagagaa tgaaaaagcg attcacattt
     2761 accgcaagct tggctttacg gttgaaggtg aattgatgca tgagtttttt attaatggtc
     2821 aatatcgtaa tgccattcgc atgtgtatat tccagcatca gtatctggca gagcacaaaa
     2881 caccgggtca gactctcctg aagccgaccg cacaatagca ttaataataa tcgatcgtat
     2941 ttttgatggt gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgatcagct
     3001 gacagtccac cggattggcg tgactgtcat attcacagct ctgttttaca ttaccaactc
     3061 gttgattatt tagtaaagta accgctgtgt aatctaattt tttgatcgga tccgttgatg
     3121 gcgtggcgct gacagataat gttttgtcgt tacttttcgt ggttttaccc agcggatacc
     3181 cctgatcatc atagcgatat tccatctgca tttgtttgct gctggcttta atcacgaagc
     3241 cattatcatc ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc
     3301 cctgtaaacg tactctcttc tccagcgtct cggcatcacg gtaataattg gcgtccagta
     3361 ccagagcgac cacggtatta ttttccagat ccagtaattc gagtgaatca aaacagcctt
     3421 cttccgacaa agtcccagaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct
     3481 gagtgaaatc ttttaccgga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg
     3541 ccatctcagg ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg aggaatatcg
     3601 cgagtaagca tggtagtaat ttgtatttca caacagtcac cagagagtag agatgattct
     3661 caatcatagt agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct
     3721 tcgcgtaagg agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc
     3781 gcctcacccg cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca
     3841 cagcgcatta tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat
     3901 gaccaggttg atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa
     3961 attctctaca accgcaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat
     4021 ggcgcgcctg ccgaaccgca gtaacacatt atcgactgaa cgccggatat gacacatccg
     4081 gcgatttgaa catacaacat aatctcacct tattactcat acccttctat tgatgtggat
     4141 taataattct taacccaaaa tgggtaggct ccctctattg ttagcgcgct aaatattcaa
     4201 tatataaaca tttatataac gataaagaac agggagtgag ttatgtccaa aaatgaccga
     4261 atggtgggca tcagccgcag aacactcgtt aaatcaaccg caataggttc tctggcgctg
     4321 gctgcaggcg gtttttcttt accgttcacc ctgcgcagtg cagcggcagc ggtacaacag
     4381 gccagcgaaa aagtgatctg gggttcctgt tccgtcaact gtggtagccg ctgtgcactt
     4441 cgtctacatg ttaaagataa tgaagtgacc tggttggaaa ctgacaatac cggcagcgat
     4501 gagtacggca accatcaggt acgcgcctgt ttgcgcggtc gctccatccg ccggcgtatt
     4561 aatcatcccg atcgcttgaa ttacccaatg aaacgcgtgg ggaaacgtgg tgaaggcaaa
     4621 ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc
     4681 gtcgaacaat atggcaatga agctgtatat attcagtact cttcggggat tgttggcggc
     4741 aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc
     4801 ggttcactca atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc catgccctac
     4861 acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg
     4921 atgtttggta acaacccggc agaaacccgc atgagcggtg gtggcatcac ttatcttctt
     4981 gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg atataccgat
     5041 acggctgccg gtcgtgaaga cgaatggctc cctattcgcc cgggcaccga tgccgcgctg
     5101 gttgcgggta ttgcctgggt attgattaac gaaaatctcg ttgatcaacc ttttctcgat
     5161 aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac
     5221 tataaagcct atattcttgg tgaaggtgac gataaaacag cgaaaacgcc gcagtgggct
     5281 tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca
     5341 acaaaacccg cttatatctg ccagggctgg gggccgcaac gccaggcaaa cggcgagctg
     5401 actgcacgcg ctattgctat gttacctatt ttgacgggca atgtcggcat cagcggcgga
     5461 aatagtggcg cgcgtgaatc gacctatacc attacgatag aacgcctacc ggtgctggat
     5521 aatccggtca aaacgtcaat ctcctgcttc agctggacgg atgctatcga tcatggcccg
     5581 caaatgacgg cactccgcga cggtgtccgg ggcaaagata aactggatgt gcccattaag
     5641 ttcatctgga acttcaagga cttgaagcgg acccttagta ggcctgaggt cctgcattga
     5701 gtcaagacct tgtgtcattc tttgtgccag acttgtgtca ttagacggga tgtctagacg
     5761 gctggtcagg acccccggtg gcctcggtct ggacttgagg tgggcctatg gggggaagtc
     5821 tgctggctta atgtattaga tggtcattca catttttctg tcagaaacca tcggtcccac
     5881 tgtgagtcca aacctaagga cctagaagtc cacccacagc cttagacaga gtgtcgaaca
     5941 catccacacc gagtcgacca gagaggacca tgtagagacc cacagagagg aacacgagtt
     6001 tatagaccag agtcaggagg gacttcaggt ctgcggaagt catagacata aagcccctat
     6061 ctattatgtt atattattac attaacaaca gatccagacc attggtccag accatagtta
     6121 gttaatcaca gtgatagccc accacctgaa gtcctgctga tattcagtcc agacttacct
     6181 ctgctcatgg tcttacctcc ctcggtctcc tgaccttggg tatcgactac agcttgctga
     6241 gcgtcatctg gaggagctac aggaggagct acaggaggcg ggaccgaagc cccaccttga
     6301 gtcccaccac cacctgtcag gcccttggcg atggacctga agcctttact aatgctctta
     6361 cccattggtc ttacgcttcg gtcttggtga acttcaggcg tttgactaca ggcaggacgg
     6421 cagtagctga gccagtgccc atcttgacgg tcgtagcagc ttcagcgaca acgaacactt
     6481 taccgaggat ttccaagtcg tgggactgag agtcgccatc acgtgaaagg acgaccgtaa
     6541 tattctcacc gtcgtgacgg aagcagccga agctcagggc cgtgaagcgt ttagccaaca
     6601 tgaacacacc tttgtcctca ccgttgtaat acgcgatgtc ggtacccagc atatccacga
     6661 tagcgtcttc accagagaag ccctgtttag cagccagacg ggacagtgct ttcagttggc
     6721 tctcatgggc gataataccg tagtcagaca cctcagaacc gaaagaaggc tcggtgttct
     6781 catacagagc gtccatgata gcgtctgcct tagcggtcag gctaccagtc aggacaggaa
     6841 gctctacggt cttcagcagc ttagaggatt ccagaacgtc gacc
//
LOCUS       DADLSA010000184         6769 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.185, whole genome shotgun sequence.
ACCESSION   DADLSA010000184 DADLSA010000000
VERSION     DADLSA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.185"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(101..739)
                     /gene="rutR"
                     /locus_tag="JN691_003705"
     CDS             complement(101..739)
                     /gene="rutR"
                     /locus_tag="JN691_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HAY7245012.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1027..2118
                     /gene="rutA"
                     /locus_tag="JN691_003706"
     CDS             1027..2118
                     /gene="rutA"
                     /locus_tag="JN691_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HAY7245013.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIIARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2118..2810
                     /gene="rutB"
                     /locus_tag="JN691_003707"
     CDS             2118..2810
                     /gene="rutB"
                     /locus_tag="JN691_003707"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HAY7245014.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2822..3208
                     /gene="rutC"
                     /locus_tag="JN691_003708"
     CDS             2822..3208
                     /gene="rutC"
                     /locus_tag="JN691_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HAY7245015.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            3216..4021
                     /gene="rutD"
                     /locus_tag="JN691_003709"
                     /pseudo
     CDS             3216..4021
                     /gene="rutD"
                     /locus_tag="JN691_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
     gene            4031..4621
                     /locus_tag="JN691_003710"
     CDS             4031..4621
                     /locus_tag="JN691_003710"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HAY7245016.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4632..5126
                     /gene="rutF"
                     /locus_tag="JN691_003711"
     CDS             4632..5126
                     /gene="rutF"
                     /locus_tag="JN691_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HAY7245017.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     SVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5147..6475
                     /gene="rutG"
                     /locus_tag="JN691_003712"
     CDS             5147..6475
                     /gene="rutG"
                     /locus_tag="JN691_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HAY7245018.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6557..6730)
                     /locus_tag="JN691_003713"
     CDS             complement(6557..6730)
                     /locus_tag="JN691_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAY7245019.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
BASE COUNT         1436 a         1826 c         1859 g         1648 t
ORIGIN      
        1 ttcgctgagt gtgaataccg ctgccgctgg cggtaacgcc agtttaatga cgatcggtta
       61 aacaggtagc cggaggatgt tacaacctcc tccggcatct ttaacgtggt cgaatcccct
      121 caataataat ccgctgcacg ttttcaaccg tttgattgaa aaatacctca tcgcgcaacg
      181 tcgcgcctgt caccgcctcc acctgagggg cgaaatcggc gtaatgttga gtggaagccc
      241 aaatcataaa aatcaaatgc tgcggatcaa tcggcgcgag tttgccgctt ttgacccaac
      301 cggcaatcag cgccgatttc tcatcaatta atgccttcaa atcgcccgtc agttcatcca
      361 ttaacaacgg cgcgcctgcc agcatctcca tacagaacag gcgcgaagcc tgcggataat
      421 cgcgtgagac ttccagcttc agacggatgt actcttttat cgccgccagc ggggcgaaat
      481 cttcacgaaa cgcttttaac ggtgccagcc agatatcgag aatctgccgc agcacggcaa
      541 tatacagcgc ctcttttgac gggaagtaat acagcagatt ggtttttgaa acacccgcca
      601 actctgcgat ctgctccagc cttgtgccgt gaaaaccgaa ttgtgaaaaa gtgtccagtg
      661 ctgcgctaag aatcgctttt ttcttcgcgc ttactgcgcg cgaacgttta cccgttgttt
      721 tcactgcgcc ttgcgtcatg cgctctcccc tcttgtagat tggtaacagg atagcaaaac
      781 ccccgcccgg cctcgacctt ctgcactctc atcgcgcacg gatgcatgtt ttatgtgcaa
      841 ctgttttgac cgtttagtcc actttttacc agatatttta gataacacaa tgctaacaca
      901 ttaataacat tcacttttta aaaactggca tccgctttgc aaacaagcca atacgcggct
      961 gaaaaggata tgcaggatgc agcgccccgt ctgactttca ctttacgaga cgaagagagg
     1021 ttgatgatga aaattggcgt attcgtacct attggcaaca acggctggct catttcgacc
     1081 cacgcgccgc agtacatgcc gacctttgaa ctgaataaag ccattgtgca aaaagcggag
     1141 cattaccatt tcgatttcgc cctgtcgatg atcaaactgc gtggctttgg cggcaaaact
     1201 gagttctggg atcacaacct tgagtcgttc accttgatgg cgggtctggc agccgtgacc
     1261 tcgcgcattc agatttacgc cactgctgcc accttaacgt tacctccggc aatcatcgcc
     1321 cgtatggccg caaccatcga ctccatctct ggcgggcgtt ttggcgtcaa cctcgtgact
     1381 ggctggcaaa agcccgagta tgagcagatg ggtatctggc ctggcgatga ctatttctcc
     1441 cgtcgttacg actatctcac cgaatatgtt caggtgctgc gcgacctgtg gggcacgggg
     1501 aaaagcgatt ttaaaggcga ttttttcacc atgaatgatt gtcgcgtcag tccgcaaccg
     1561 agtgtcccca tgaaagtgat ctgcgccggg caaagcgacg ctggcatggc gttctccgcc
     1621 cagtatgccg atttcaactt ctgtttcggc aaaggcgtaa atacacccac ggctttcgcc
     1681 ccgaccgctg cgcggatgaa acaggccgca gagcaaaccg gacgcgacgt tggctcttat
     1741 gtgttgttta tggtgattgc cgatgaaacc gacgatgcca ctcgcgccaa atgggaacac
     1801 tacaaagcgg gcgcggatga agaggcgtta agctggctaa ccgaacaaag tcagaaagat
     1861 acccgctccg gtactgacac caacgttcgt cagatggccg atcccacttc ggcggtaaac
     1921 atcaatatgg ggacgttagt cggttcttac gccagtgtcg cgcgcatgtt agatgaagtc
     1981 gcaagcgtgc ctggtgcaga aggcgtgctg ttgaccttcg atgattttct gtcgggaatc
     2041 gaaaccttcg gcgagcgcat tcaaccactg atgcagtgcc gcgcccatct ctctgcgctg
     2101 actcaggagg tggcatgatg acaaccttaa ccgctcgacc ggaagccatt accttcgatc
     2161 cgcagcaaag tgcgcagatc gtggtggata tgcaaaacgc ttatgccacg ccaggcggct
     2221 acttagatct cgccgggttt gatgtctcaa ccactcgccc ggtcattgcc aacattcaaa
     2281 ccgctgtgac cgcagcgcga gcggcaggga tgttgatcat ctggtttcaa aatggctggg
     2341 atgaacagta tgtcgaggct ggcggccccg gctcaccgaa ttttcataaa tcgaacgccc
     2401 tgaaaaccat gcgtaagcag ccgcagctgc aggggaaatt gctggcgaaa ggctcctggg
     2461 attatcaact ggtggatgaa ctggtgccgc agcctggcga tattgtgctg ccgaagccgc
     2521 gctacagcgg tttcttcaat acgccgctgg acagcatttt gcgcagccgt ggaatacgcc
     2581 atctggtttt caccggtatc gctaccaacg tctgcgtcga atcgacgcta cgcgatggct
     2641 tttttctgga gtatttcggc gtggtgctgg aagacgcaac acaccaggcg gggccggaat
     2701 ttgcacagaa agccgcgttg ttcaatatcg aaaccttttt tggctgggtc agcgacgtcg
     2761 aaacgttctg cgacgcgctt tctcccacgt cctttgctcg tatcgcttaa ggagtttaac
     2821 gatgccaaaa tccgtaatta ttcccgctgg cagcagcgca ccgctggccc ccttcgttcc
     2881 cggcacgctg gctgatggcg tggtgtatgt ctccggtacg ctggcttttg atcaacataa
     2941 taacgtgctg tttgccgatg acccaaaggc gcaaacccgc cacgttctgg aaactatccg
     3001 caaggtgatc gagacggcgg gtggcacgat ggcggatgtg accttcaaca gcatctttat
     3061 taccgactgg aaaaattacg ccgcgattaa cgaaatctac gccgagtttt ttccgggtga
     3121 taaaccggcg cgattctgca ttcagtgcgg actggtaaaa cctgacgcgc tggtggaaat
     3181 cgccacaatt gcgcatatcg ccaagtgagg ccgcgatgaa actttcactc tcacctcccc
     3241 cttatgctga tgcacccgta gtggtgttga tttcgggtct tggcggtagc ggcagttact
     3301 ggttaccgca actggcggtg ctggagcagg agtatcaggt agtctgttac gaccagcgcg
     3361 gcaccggcaa taatcccgac acactggcag aagattacag tatcgcccag atggcagcgg
     3421 aactgcatca ggcgctggta gccgcaggga ttgagcgtta cgcggtggtc ggccatgcgc
     3481 tcggtgcgct ggtggggatg cagctggcgc tggattatcc cgcgtcggta actatgctgg
     3541 tcagcgttgc gttaacggct ggctacgaat aaacgcccat acgcgccgct gttttcaggt
     3601 tcgcgaacga ttactgtata gcggcggcgc gcaagcatgg gtggaagcgc agccgttgtt
     3661 cctctatccc gccgactgga tggcggcccg cgcacctcgc ctggaggcag aagacgcgct
     3721 ggcactggcg cattttcagg gcaaaaataa tttactacgt cgacttaacg ccctcaaacg
     3781 cgctgacttt agtcaccatg cggatcgcat ccgctgcccg gtgcaaatca tctgcgccag
     3841 tgatgatctg ctggtgccaa cagcatgttc cagtgaactt catgccgccc tgcccgatag
     3901 ccagaaaatg gtgatgccct atggcggaca cgcctgcaac gtgaccgatc ccgaaacgtt
     3961 taatgctctg ttactcaacg ggcttgccag cctgttacat caccgtgaag ccgccctgta
     4021 aggaattgct atgaacgaag ccgttagccc aggtgcgctt agcaccctgt tcaccgatgc
     4081 ccgcactcac aacggctggc gggagacacc cgtcagcgat gagacgttac aggagcttta
     4141 tgccctgatg aaatgggggc cgacatcagc taactgttct ccggcacgga tcgtgtttat
     4201 ccgcactgca gaaggaaaag aacgtctgcg cccggcgctt tccagcggca atctgcaaaa
     4261 aaccctgacc gcgcccgtca ccgctatcgt cgcctgggac agtgaatttt atgaacggtt
     4321 accactactg tttccccacg gtgatgcccg cagttggttt acctccagcc cacaacttgc
     4381 cgaagaaaca gcgtttcgca acagttccat gcaggcggcc tatctgatcg tcgcttgccg
     4441 tgcgctggga ctggataccg gcccgatgtc gggttttgac cgtcaacacg ttgacgacgc
     4501 cttttttgcg ggcagcacgc tgaagagcaa tctgctgatt aatatcggct atggcgatag
     4561 cagcaaactt tttgcgcgcc tgccacgtct gtcctttgaa gaagcctgcg ggctgttgta
     4621 aggagcaaac tatgaacata gttgatcaac aaacctttcg cgatgcgatg tcctgtatgg
     4681 gcgcggcggt caatatcatc accacggacg gtccagccgg gcgcgccggg ttcaccgcca
     4741 gcgccgtctg cagcgtgacc gacacgccgc ccacattact ggtgtgcctg aatcgtgggg
     4801 cgtccgtctg gccggtattc aatgaaaatc gaacgctgtg tgtaaataca ctgagcgccg
     4861 ggcaggagcc gctttcaaat ctttttggcg gcaaaacgcc catggaacac cgctttgccg
     4921 ccgcccgctg gcagacgagt gtgaccggat gcccgcaact ggaagaggct ctggtttcgt
     4981 ttgactgccg tatcagccag gtggtgagcg tcggcaccca cgacattctg ttttgcgcca
     5041 tcgaagcgat tcatcgtcac accacaccct acgggctggt gtggttcgat cgcagttatc
     5101 acgcgctgat gcgccctgct tgttaacctc cttaaggaga cagctgatgg caatgttcgg
     5161 ttttcctcac tggcagttaa aatcgacctc tacagagagc ggcgtggtcg cgccggatga
     5221 acgattgccg tttgctcaga cggcagtaat gggcgttcaa cacgcggtgg cgatgtttgg
     5281 cgcgacggtg ttaatgccga ttctgatggg gctggatccc aatctttcca ttttaatgtc
     5341 ggggatcggc acgttactgt ttttctttat taccggcggg cgcgtcccca gttatctcgg
     5401 ctccagcgcg gcttttgtcg gcgtggtgat tgccgcgacg ggctttaacg gtcagggcat
     5461 taacccgaat atcagcatcg cgctgggcgg gattatcgcc tgtgggctgg tttataccgt
     5521 cattggcctg gtggtgatga aaattggtac acgctggatt gaacgactaa tgccgccggt
     5581 ggtgacgggt gcggtggtga tggcgattgg cctgaactta gcgcctatcg cagtgaaaag
     5641 tgtatcggct tcggcctttg atagctggat ggcggtaatg acggtgctgt gtattgggct
     5701 ggtggcggtg tttacccgtg gcatgatcca gcggttattg attctcgtag gattaattgt
     5761 cgcctgtctg ctgtatggcg tgatgaccaa cgttttaggg ctgggaaaag ccgtcgattt
     5821 tactctcgtc agctatgctg cctggttcgg tctgccgcat ttttccactc ctggttttaa
     5881 tggccaggcg atgatgttga ttgcgcccgt ggcggtgatt ctggtggcgg aaaacttagg
     5941 tcatctcaag gcggtcgccg ggatgaccgg acgcaatatg gacccgtata tggggcgagc
     6001 attcgtcggt gatggtctgg caacgatgct ttccggctct gtcggcggta gcggcgtcac
     6061 cacctatgct gaaaatatcg gtgtgatggc cgtgacaaaa gtctactcga cgctggtgtt
     6121 tgttgcagct gccgtcatcg ctatgctgtt gggcttttcg ccgaaatttg gcgcgctgat
     6181 ccataccatt ccggccgcgg tgattggtgg tgcatcaatt gttgtgttcg ggctgattgc
     6241 cgtcgcaggc gcaaggatat gggtacaaaa ccgtgtcgat ttaagccaga acggtaattt
     6301 gattatggtc gcagtaacct tcgtgctggg cgcgggtgat tttgctctca cgcttggcgg
     6361 ttttacgttg ggagggatcg gtacagcaac ctttggcgcg attttactca atgcgttatt
     6421 aagcagaagg ttggttgacg ttccaccgcc agaagttgtt catcaggagc cataaacaga
     6481 aaaccgccgg gttaccggcg gtgatgactt tttgacgagc ttaagaaaaa aatgtattcg
     6541 tcattgatta gcccggctag ttgtcgcttt tgccgtgact gctcttgcca ccttttttac
     6601 ctgcttcaga tgcgcgctgc ggatcattct tgaaattacc cccgctgtgc tgtccacctt
     6661 ttttacctgc ttctgatgct ctttcgcggt cttctgcaaa attgccggaa ccgcctcgat
     6721 ggtttgccat tactgacctc cgtctgggta tagacatcat attgcatag
//
LOCUS       DADLSA010000185         6713 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.186, whole genome shotgun sequence.
ACCESSION   DADLSA010000185 DADLSA010000000
VERSION     DADLSA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.186"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            332..1987
                     /locus_tag="JN691_003714"
     CDS             332..1987
                     /locus_tag="JN691_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HAY7245020.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            2127..2351
                     /locus_tag="JN691_003715"
     CDS             2127..2351
                     /locus_tag="JN691_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HAY7245021.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            2414..2950
                     /gene="rimL"
                     /locus_tag="JN691_003716"
     CDS             2414..2950
                     /gene="rimL"
                     /locus_tag="JN691_003716"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HAY7245022.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(2945..3925)
                     /locus_tag="JN691_003717"
     CDS             complement(2945..3925)
                     /locus_tag="JN691_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HAY7245023.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWLDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            4049..5041
                     /gene="tehA"
                     /locus_tag="JN691_003718"
     CDS             4049..5041
                     /gene="tehA"
                     /locus_tag="JN691_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAY7245024.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            5038..5631
                     /gene="tehB"
                     /locus_tag="JN691_003719"
     CDS             5038..5631
                     /gene="tehB"
                     /locus_tag="JN691_003719"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAY7245025.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            5934..6602
                     /locus_tag="JN691_003720"
     CDS             5934..6602
                     /locus_tag="JN691_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HAY7245026.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(6620..>6713)
                     /locus_tag="JN691_003721"
     CDS             complement(6620..>6713)
                     /locus_tag="JN691_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY7245027.1"
                     /translation="QQTPGQVENRALYPRPEGWGFTAHRIKSAI"
BASE COUNT         1718 a         1556 c         1642 g         1797 t
ORIGIN      
        1 ccagtcgcga agcatttgag caggcgttgg gttgtccggt tttggatgag ttccagcttt
       61 accaggaagc ggaagaacgc agtaaacgtc gctgtggttt gctttaaatt ctgaccatcc
      121 gcctttgcaa aaatttgcct gattttacaa acgaatcagg ctcatcccat cgacataaaa
      181 aaaatgccga tttatgcata ttctctcagt tcaacaattg gattattaat aaatattgtc
      241 tagagtgagc ggtcataaat aagcactttc ttgccgctga aaacgaccag cgcgggacca
      301 ttcacaacac cagaaggact cactttcagg tatggatcgt agacgattta ttaaaggttc
      361 aatggctatg gccgccgtgt gcggtaccag cggcattgct tctctttttt ctcaggcggc
      421 attcgcggca gattctgata ttgccgacgg gcaaacccag cgttttgact tctccattct
      481 acagtcaatg gcgcacgact tagcgcaaac agcgtggcgt ggtgcgcctc gtccgttacc
      541 tgacacgctg gcgacaatga cgccgcaggc ttataacagt attcaatacg acgccgaaaa
      601 atcgctctgg cataacgttg agaaccgtca actggacgct cagttcttcc atatgggaat
      661 gggattccgt cgccgcgttc gtatgttttc tgtagatcca gcaacacatc tggcgcgtga
      721 aattcacttt cgcccggagt tgttcaaata caacgatgca ggtgttgata ccaaacaatt
      781 agaagggcaa agcgatctcg gctttgccgg ttttcgcgtg tttaaagccc ccgaactggc
      841 gcgccgtgat gtagtatcat ttctcggcgc gagttatttc cgcgccgttg atgatacata
      901 tcaatacggt ttgtcggccc gcggcctggc gatcgacact tacaccgaca gtaaagaaga
      961 gttccccgac tttaccgcct tctggtttga tacggtaaaa ccgggggcaa ctacctttac
     1021 cgtttatgcg ttgctcgata gcgccagcat tactggtgcc tataagttca ctatccattg
     1081 tgagaaaagt caggtgatta tggatgtgga aaatcacctg tatgcgcgca aagacattaa
     1141 acagctgggc attgcgccga tgaccagtat gttcagctgc ggtactaatg aacgtcggat
     1201 gtgcgataca attcatccgc aaattcatga ctctgatcgt ctgtccatgt ggcggggcaa
     1261 tggcgagtgg atttgccgtc cgctgaataa tccgcaaaaa ttgcagttca atgcttacac
     1321 cgacaacaac ccgaaagggt ttggtttatt gcaactggat cgtgacttct cccattatca
     1381 ggacattatg ggctggtata acaaacgccc aagtctgtgg gtggaaccgc gtaacaagtg
     1441 gggtaagggc accatcggcc tgatggaaat cccaacaacg ggcgaaacgc tggataacat
     1501 tgtctgcttc tggcagccag aaaaagctgt aaaagcaggt gatgagtttg cattccagta
     1561 tcgtctgtac tggagtgcgc aaccgcctgt tcattgccca ttagcgcgcg ttatggcgac
     1621 gcgtaccggc atgggcggtt tcccggaagg ttgggcgcca ggtgaacact atcccgaaaa
     1681 atgggcgcgt cgttttgccg tcgatttcgt tggtggtgat ctgaaagctg ccgcgccaaa
     1741 aggcattgag ccggtgatta cgctttccag tggggaagcg aagcaaatcg aaattctcta
     1801 tattgaaccc atcgatggtt atcgtattca gtttgactgg tatccgactt cggactccac
     1861 tgatccggtc gatatgcgga tgtatctacg ttgtcagggg gacgctatca gtgaaacatg
     1921 gctgtatcag tatttcccgc cagcgccgga taaacgtcag tatgttgacg accgcgtgat
     1981 gagttaatcg ttttttcttc ggcaccttct tcgggaggtg ccgtctggtt aaacacgatc
     2041 ccgctcgcat ttttccctaa gttaaatgag taatctgatg gtgtgtattt cagatacacc
     2101 gtgtcagcca ctaacaggga gtgcgtatgt ttccagaata ccgagattta atatcccgtc
     2161 tgaaaaacga aaatcctcgc tttatgtcct tgttcgataa acacaataaa cttgatcatg
     2221 aaattgccag aaaggaaggt tccgacggtc gagggtacaa tgcggaagtg gtccgcatga
     2281 aaaaacaaaa gctacagtta aaagatgaga tgctcaaaat cctgcagcag gagagcgtca
     2341 aagaggtgta aactttccta agccgccagc caggcggctt ttttaacaac agcatggatt
     2401 gactggagat aagatgactg aaacgataaa agtaagcgaa tcacttgaat tacatgctgt
     2461 tgcggaaagt cacgtcacac ctctttatca gttaatctgt aaaaataaaa cctggttaca
     2521 gcagtcgcta aactggccgc agttcgttca gagtgaagag gacacgcgaa aaacggtgca
     2581 gggtaatgtg atgttgcatc aacgcggtta tgccaaaatg ttcatgattt tcaaagaaga
     2641 tgaacttatc ggcgttatct cgtttaatcg tattgaacca ctgaataaaa ctgctgaaat
     2701 cggctactgg ctggacgaat ctcatcaggg gcaggggatc atttctcagg cgctgcaggc
     2761 attgattcat cattacgctc agtctggtga acttagacgc ttcgtgatca aatgtcgggt
     2821 ggacaatccg caaagcaacc aggtcgcttt gcgcaatggt tttatccttg aaggttgcct
     2881 gaaacaggct gagttcctga atgatgccta tgatgatgtg aacttatacg cgcgtattat
     2941 cgattcataa taaccctgcc agcggcgttc gcgtaatgcg ctcctcgccg ttaatgactt
     3001 ttggaccacg gacatgaacc gtgtcgccat caaaggcttc aacgacggcg tggtcggtta
     3061 actccacatg gttttcaatt atcaccgcac ctgtaatacg gctctcacct tgtattacaa
     3121 cgtgctcgtc gagcagaatc ggtccaccac gtaccacggc atttccgcca atcaagacat
     3181 gatgttttaa cacacaatta ccttccacaa tggcatattc cgccacctgc gaactgtaat
     3241 gaatcgtcgg aatggcatct tcttctatgc cagctttcac ctgcgcgtga ccgtagactt
     3301 tagcgcaatc gcatagccag acgttgttct cttcattacc ttcaacactg gcaaaatcaa
     3361 aaacttcggc gcgatgttca ataaaagcat accggacgac ggcatcgcca taaatttgtg
     3421 cctggtggac aatacgcgag gcgctaaccc ttgcgcgatc ataaatttgg agcaggagtt
     3481 gatggtcggg cgttaagcct tgtgcggcga cgatcataga atgttgatca attaaggcgt
     3541 gaccaaatat tctacattgc ccgtaaacta atgagtcatg aatggtgaca ctgtcactta
     3601 tatatgcacc ctggctgatt tcactattat cgatccagac gttatccgtt gcgtaaacct
     3661 ctccccacag cacgctggtg ccggtgatac gcgtattacc ggaaatcact gttccgccga
     3721 acgcaatagc attctggtcg taaatccagc aatttccttc ctgtgctaac accgtttcgc
     3781 gatcgagcca gccgccagcg gttcctgcta tcacatcgtt aaaatcgcta atggcgataa
     3841 tctgccgtaa taacacactt tttttagtgc catcttcctg ataactaaag gctcgctgtt
     3901 cttcacttag gcgatatttg cgcatagcgt tttcccacag gtgacttact ataaccgtag
     3961 caaattctgc ggctctggct atgctcgaga aaattccata aaatgcattt caaatatact
     4021 ttataaatta aacaaaatga gtaagaagat gcagagcgat aaagtgctca atttgccggc
     4081 aggctacttt ggtattgtgt tggggacgat agggatggga tttgcctggc gctatgccag
     4141 ccaggtttgg caggtcagcc actggttagg ggatgggctg gtgattctgg cgatgatcat
     4201 ctggggatta ttgactagcg catttattac ccgactcata cgctttccgc atagcgtgct
     4261 ggcggaagtt cgccatccag tgctgagcag ttttgtgagt ttgtttccgg caacgacgat
     4321 gctggtggcg attggttttg ttccgtggtt tcgcccactg gcggtgtgca tgttcagttt
     4381 tggtgtcgtg gttcagttgg cttatgccgc ctggcaaact gcgggattat ggcgcggatc
     4441 tcaccctgaa gaagctacca cgcctggact gtatctgccg acagttgcca acaactttat
     4501 cagcgcaatg gcctgtggtg cgttgggcta caccgacgcc ggtctggtgt ttttaggcgc
     4561 aggcgttttc tcatggctaa gccttgaacc ggtgatcttg cagcgtctgc gcagttcggg
     4621 agaattaccc acggcactgc ggacatcact cggcattcag ctcgctcctg cgctggtggc
     4681 ctgtagtgcc tggctgagcg tcaacggcgg cgagggtgac acgctggcga aaatgctttt
     4741 cggttatgga ctgctgcaac tgctgtttat gctacgtctg atgccatggt atctctccca
     4801 gccatttaat gcttcattct ggagtttctc gttcggcgta tctgcactgg caaccaccgg
     4861 tttgcatctg gggagtggca gcgataatgg atttttccat acgctggcgg tgccgctgtt
     4921 tatctttacc aattttatta ttgcaatact gctcatccgt acttttgcgc ttctgatgca
     4981 gggaaaattg ttagtcagaa ccgagcgcgc cgttttaatg aaagcagagg acaaagaatg
     5041 atcattcgtg acgaaaacta ttttactgat aaatatgaat taacccgcac acactctgaa
     5101 gtactggaag cggtgaaagt ggttaaaccg ggtaaaacgc tggatctggg ctgtggcaat
     5161 ggtcgtaaca gtctttacct ggcagccaat ggttatgatg ttgacgcatg ggataaaaat
     5221 gccatgagta tcgccaacgt cgagcgcatt aaatccattg aaaatctgga taatttacac
     5281 acccgggtcg ttgatctgaa taacctcaca tttgatggac agtacgattt tattctttcg
     5341 actgtggtgc tgatgttcct tgaggctaaa accatcccag ggttgattgc caatatgcaa
     5401 cgttgcacta aacctggtgg ttacaacctg attgtggcgg cgatggatac cgctgattat
     5461 ccatgtaccg tcggcttcac gtttgccttc aaagagggag aattacgtcg atattacgaa
     5521 ggctgggaga gggtgaaata caatgaagac gtcggcgagc tgcaccgcac cgacgccaac
     5581 ggtaatcgta ttaaactgcg tttcgccacg atgctggcac gtaaaaaatg acccggtaag
     5641 cacaaaacgc gtgaaaattc cccacgctga gatgatttac tgttcttctt ttcggtaagc
     5701 atatttttta tcgaagggat gtgaaattaa tcacagtagt cgaagttttt tagcagctta
     5761 acttactgaa atttaactac tgatgattga cttagcccct ttttcggcat tgactatgtc
     5821 gtctgaaaag gggctgaaaa atttattttc accaacactt tttttgccac aacacgaagc
     5881 ggcgcttttt gctataactt agaaagtaat ataatcatct caggaaacta ttcatgcgta
     5941 ccacatcatt tgcgaaagtt gcagctttat gcggcttgtt ggctctgtct ggttgtgcct
     6001 ctaaaatcac ccagccagat aaatattctg gttttttaaa caattactct gatttaaaag
     6061 aaacaacctc agcaacaggt aaacctgttt tacgttgggt agacccgagt tttgatcaaa
     6121 gcaaatatga cagcatcgtc tggaacccaa tcacttatta tccggtacca aaaccttcta
     6181 cccaggtagg gcagaaagtt ctggataaaa ttttgaacta taccaacacc gaaatgaaag
     6241 aagcgatagc gcagcgtaaa ccagtggtta ccaccgctgg gccgcgtagt ctgattttcc
     6301 gtggggccat taccggtgta gataccagca aagaagggct gcaattctat gaagtggttc
     6361 ctgttgcatt agtggttgcg gggacgcaaa tggctacagg ccaccgtacc atggatactc
     6421 gcctctattt tgaaggtgag ctgattgatg cagcgactaa taaaccggtt atcaaagtcg
     6481 ttcgtcaggg cgaaggtaaa gacctgaata acgaaagtac gccaatggct ttcgaaaata
     6541 ttaaacaagt tattgatgac atggcgaccg atgccaccat gtttgacgtt aacaaaaagt
     6601 agttccagac gcgccatcgt tagatggcgc tttttatccg gtgcgccgta aaaccccatc
     6661 cttcagggcg gggatataag gcgcggtttt ccacctgacc aggtgtttgc tgt
//
LOCUS       DADLSA010000186         6641 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.187, whole genome shotgun sequence.
ACCESSION   DADLSA010000186 DADLSA010000000
VERSION     DADLSA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.187"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(1..800)
                     /locus_tag="JN691_003722"
                     /pseudo
     CDS             complement(1..800)
                     /locus_tag="JN691_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(810..1601)
                     /locus_tag="JN691_003723"
     CDS             complement(810..1601)
                     /locus_tag="JN691_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HAY7245028.1"
                     /translation="MKSTRPVAVITGAARGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            1813..2460
                     /locus_tag="JN691_003724"
     CDS             1813..2460
                     /locus_tag="JN691_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7245029.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKVIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            complement(2444..2989)
                     /locus_tag="JN691_003725"
     CDS             complement(2444..2989)
                     /locus_tag="JN691_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245030.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            3208..3828
                     /locus_tag="JN691_003726"
     CDS             3208..3828
                     /locus_tag="JN691_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7245031.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            4136..5548
                     /gene="cycA"
                     /locus_tag="JN691_003727"
                     /gene_synonym="dagA"
     CDS             4136..5548
                     /gene="cycA"
                     /locus_tag="JN691_003727"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAY7245032.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(5593..6255)
                     /gene="ytfE"
                     /locus_tag="JN691_003728"
     CDS             complement(5593..6255)
                     /gene="ytfE"
                     /locus_tag="JN691_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAY7245033.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6363..>6641)
                     /locus_tag="JN691_003729"
     CDS             complement(6363..>6641)
                     /locus_tag="JN691_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HAY7245034.1"
                     /translation="PLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETL
                     AGLLYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
BASE COUNT         1538 a         1652 c         1731 g         1720 t
ORIGIN      
        1 ctgttaatat ctttctgcca ccagtaaggc gagaatattt ccagttcaat agcaccatta
       61 aatccggcgt tttcaaccag ccgacgaatt gacggaatat tgatgacgcc atctcccggc
      121 ataccacggt cattgaccag atcagtggtc ggcactaacc agtcggaaac atgaaacgcg
      181 aggatgcgtt ttccggcacg caggatctga ctggcgagat ccggatccca ccagacgtga
      241 taaacatcca ccgccacgcc gaggccaaac tcaccatcgg gatcgagttc atcacaccag
      301 tccagcgcct gacgtaacgt gcacaggcaa gagcgatcgg cagctgtcat cgggtgcagt
      361 ggctccagcg caattggcaa gccgacatct ttcgaatgtg gcagcaactg gcgaatccct
      421 tgtttcactt gttctcgcgc ttcatataga tctttagtgc ccgttggcag accgccaacc
      481 acctgcatat aacaggcggc gttaagcacc gctgcgtcgt ccagcgcccg gcggttatca
      541 tcaatcgcca gtttgcgttc cgccagtgtc ggcgcggtgt agtaagtgct acggcacaaa
      601 ccggaaacgc tcatgttact ggcggcaagc tggcgggtga tttgctggag atcttcaccc
      661 tacaactccc tgcgccaggg ggcaattgcg ccaataccgc gtgcggcgca ggcatcaata
      721 atggcgggta gcggcgtttt atagcccagt gtcgcggtat tgatactgaa taaatccggg
      781 cgttgtaaca gatctctcat gattcagcct cagaagcgcg gcatacttaa gccgccgtcg
      841 attgcgaccg cctgaccaca ggtgtaaatc agtttacctt ccgccatcgc gcggacggtg
      901 gaagcaatat cagccggata accccaacgt ccccacggca ccagaccttt ggcaatcagt
      961 tcgtcgtaat aagccgttgc cggaatagtc atatcagttt tgatgagacc agggcgcact
     1021 tcataaacgc caatttgttc attacaaagg cgagcagcaa acagacgagc agcggcagaa
     1081 acggcggttt tggcaatggt gtattcgccg cgattcatcg ccagcatgat ggcgttgatg
     1141 gaactgacga aaataatcga gcggtgcggc agctccgctt ccggtttcgg ctgtgccagt
     1201 aagcgcttac tgaatgcctg agcaaggaag aacggcgcgc gggtgttaat ggcaatgttt
     1261 tgatcgaagc tgtctggttc gaggtcgagg aggtcaccgc gttttttcac tgaaatcccc
     1321 gcgttattga gcagacagtc cagccgcccc cactggtttt gcgccgcgtc gagcatctcc
     1381 tcatgcagag atagatcacc gacatcagca gggaagcaaa tcacttccac gccttccgcc
     1441 aaacattctt gttgtgtgat atgcagtttt tctacgctgt cggcatcggg acgatcgtta
     1501 atcagcaggt taaacccgcc acgagcaagc tcctgcgcac agccctttcc aataccgcga
     1561 gcggctccgg taatcacggc aacaggacga gtggatttca tacaatagct ctcttttttg
     1621 aattttgttt ccttggtgat gattcgcttg cgactggagg ataaaaagac caaccatcta
     1681 gttggttgat atgcgatggc gatcacagga catttgataa cctgatgaaa gtacaagttt
     1741 ttgccctttt ttctgaagct gaaatcaaat caggttatga tagtcaccac gaagaggtcg
     1801 tggggaagcg ttatgtatat tgataccagc aacgtacaga gcctgaagga aaaattgctg
     1861 ctatgtgcgg ttaatgaatt tgccgaatat ggttatgaag gggccagagt agataatatt
     1921 gtaaaggcag cagggtgtag taaacagacg gtttatcacc actttggtaa taaagaaaat
     1981 ttatttatcg aagtgctgga atatacctgg aacgatattc gacaaaaaga aaaggccctt
     2041 gatttctcag atcttccgcc acaaaaagcc attgagaaag ttattgattt tacatgggat
     2101 tattatattg ctaatccttg gtttttaaaa attgtgcata gtgaaaatca aagtaaaggt
     2161 gtgcattatg caaaatcaca gcgtctctta gaaattaacc atgcgcactt gcagttaatg
     2221 gaatccttat tggatgaagg gaagaaatac aatattttta agccaggtat cgacccattg
     2281 caggtgaata ttaatattgc tgcgcttggg ggatattatt tgatcaacca gcatacgctt
     2341 ggcctggttt atcatatcag tatggtttcc ccccaggcac tggaagccag acgtaaggtc
     2401 atcaaagaga caatccttag ctggcttttg gttgaccctt catctaccgc gcacgaataa
     2461 aactgccatc cggctggcgg gtaaacagga cctgttgatt attccccgta tcaatggtta
     2521 agcccgtcac cacgccgctg gcgttctggc ggattttcac catctgccca ttttgcaaat
     2581 tactcagcgg cttacccgcg ccttctacct gcgccattgc atagacatcg gtagcgggca
     2641 gcccgtggtc gcggaacagc tgcgccatcg ttttaccggg ttcgacacgg taagagcgcc
     2701 attgattatc aattccgcta tctggctgga atggttgcgt ttgggtagtt tgcggctgtt
     2761 cttctgtcgg cggttgtgat tgcgactgaa tatctaactg cgcttcacgc gtcaccaccg
     2821 gcgcgttggg cgtatcatca gatggaagca gaaatccgac caccaataca atggcggcaa
     2881 taatgatccc ccgacgatgc atcggcggca gcgggtccat gaagcgaaaa ttgtccggcg
     2941 cgtgccagac tttctccagg gttggtttta gttcaaagcg cccgggcatg gtttcctcct
     3001 gctccacgtc ttgttcctca atcatagcct atgaataagc taacgctatg atgtccgtgg
     3061 taaacccgcc tttattatat tcatacggga tattgctatt gtttcttttt ccccgggatt
     3121 tgtcatcatt cctgcgacaa agttttaccc aaagaagtgt ggctgatatg ctgcccgcta
     3181 cttttatacc ctaagaaagg aaatacgatg accaccccaa cttttgacac catcgaagcg
     3241 caagcaagct acggcattgg tttgcaggta gggcaacaac tgagtgaatc tggcctggaa
     3301 gggctgctgc cagaagcact ggttgcaggt attgccgatg cgctggaagg caaacatccg
     3361 gctgtgccgg ttgatgtggt gcatcgcgcg ctgcgtgaaa tccacgaacg cgccgatgcc
     3421 gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg tgaaatacct ggaagaaaac
     3481 gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc aattccgcgt gatcaaccag
     3541 ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg ttcattacac cggtaaactg
     3601 atcgacggca ccgtgtttga cagctccgtt gctcgtggtg aacccgctga attcccggtt
     3661 aatggcgtga tccctggctg gattgaagca ctgactctga tgccggtagg ttctaaatgg
     3721 gaactgacta tcccgcagga actggcatat ggcgagcgcg gcgcaggtgc atccatccct
     3781 ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa tcctctaagc agcgcattct
     3841 gttcccctcg aaccagaggg gagcaggcat tcagcaataa acccttcagt ttgccaaacg
     3901 gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt ttgtgagctg tttcgcgtta
     3961 tcaccgtgat atgacactca ctttaaacat aaaattaaca ttagatctaa atcttagtat
     4021 tcatcccgcg tattgttacc taatatcgat gagtcccgat acagattcgt cgtatcatag
     4081 actgactaaa ggccgtagag cctgaacaac acagacaggt acaggaagaa aaaacatggt
     4141 agatcaggta aaagtcgttg ccgatgatca ggctccggct gaacagtcgc tacggcgcaa
     4201 tctcacaaac cgacatattc agcttattgc cattggcggt gccattggta ctgggttgtt
     4261 tatggggtcc ggcaaaacga ttagccttgc cgggccgtcg atcattttcg tttatatgat
     4321 cattggtttt atgctctttt tcgtgatgcg ggcaatgggg gaattgctgc tttcgaatct
     4381 ggaatacaaa tcttttagtg acttcgcttc cgatttactg gggccgtggg cgggatattt
     4441 caccggctgg acttactggt tctgctgggt tgtaaccggt atggcagacg tggtggcgat
     4501 cacggcttat gctcagttct ggttccccga tctctccgac tgggtcgcct cgctggcggt
     4561 gatagtgctg ctgctgacgc tcaaccttgc caccgtgaaa atgttcggtg agatggagtt
     4621 ctggtttgcg atgatcaaaa tcgtcgccat cgtgtcgctg attgtcgtcg gcctggtcat
     4681 ggtggcgatg cactttcagt caccgaccgg tgtggaagcg tcattcgcgc atttgtggaa
     4741 tgacggcggc tggttcccga aaggtttaag tggcttcttt gccggattcc agatagcggt
     4801 tttcgctttc gtggggattg agctggtagg tacaacagct gcggaaacca aagatccgga
     4861 gaaatcactg ccacgcgcga ttaactccat tccgatccgt atcattatgt tctacgtctt
     4921 cgcgctgatt gtgattatgt ccgtgacacc gtggagttcg gtagtcccgg agaaaagccc
     4981 gttcgttgag ctgtttgtgt tggtaggttt gcctgcggct gccagcgtga tcaactttgt
     5041 ggtgctgacc tctgcggcgt cttccgctaa cagcggtgtc ttctctacca gccgtatgct
     5101 gtttggtctg gcgcaggaag gtgttgcgcc gaaagcgttc gctaaacttt ctaagcgcgc
     5161 agtacccgcg aaagggttga ccttctcgtg tatctgtctg ctcggcggcg tggtgatgtt
     5221 gtatgtgaat cctagcgtga ttggcgcgtt cacgatgatt acaaccgttt ccgcgattct
     5281 gtttatgttc gtctggacga ttatcctttg ctcgtacctg gtgtaccgca aacagcgtcc
     5341 tcatctgcat gaaaagtcga tctacaagat gccgctcggc aagctgatgt gctgggtatg
     5401 tatggcgttc tttgtgttcg tggtcgtgtt gctgacactg gaagatgaca ctcgccaggc
     5461 gctgctggtt accccgctgt ggtttatcgc gctggggttg ggctggctgt ttattggtaa
     5521 gaagcgggct gctgaactgc ggaaataacc gcattatcat gctggatggc gcaatgccat
     5581 ccagctttta gatcactcac ccgctagggc gcgtgggaac agtacattgt tttccagact
     5641 gatgtgatcc atcaggtcat caatcagttc attaatgccg ttatacatcg ctttccaggt
     5701 ggtgcaggct tctggcggcg gtgtgacgtt attggtagtg tgtttgatca cttccagcag
     5761 ttcgcccgct tcatcgtgct cgctttccat tacgctgatt ggccccattg cctggctgcc
     5821 catgccttgt ttgatcatcg ggaagaggat ctgctcttct ttcatcatgt ggctggaaag
     5881 ctcttcatgc agcatggtca ggtattttgt cagccctttt ggcacgctcg gtttgtcggc
     5941 gtgaacgcgc tcgactttag tcgcttgcag gatcagctcc ggcagctgct cgcggtgacg
     6001 atcgtggtag cgcacgatga tatggtcaat gatttctgcc agcggggcgc tacgccagtc
     6061 tttctcaatc ggttgttcag caagctttgc cagttcagct tcaatgacct caacatccag
     6121 ttctttacgt gccgccgcgc gcgccagcgt ctgcttaccg ccacagcagt aatccatatc
     6181 atatttacga aacagagccg aagcgcgagg gatagagagc gccagttcac ctaaaggttg
     6241 gtcgcgataa gccatagctg atacctcatt cttaataaga taagatgtat tttaaataca
     6301 cctttaaggc aaaagctata acccttacgt agcagaggct tatttaactc actgccagca
     6361 tgtcaacttt ctgagacaga ttcagttaaa ggtttttctg gttttgctct gaccgcgatg
     6421 accacgccaa tcactaacag cgcgataccg ctcagcgtca ttagcggcgg catttgctgg
     6481 cggagtaaaa aggtgtacag caaacctgcc agcgtttcga aaacaatcag cggcccgaga
     6541 atcactgtcg gtaatcgctg gctggcgacg ttccagcaga gtgcgccaac ccatgagcaa
     6601 agcacggcta tcgcaaccat cagactaata aacaccagcg g
//
LOCUS       DADLSA010000187         6545 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.188, whole genome shotgun sequence.
ACCESSION   DADLSA010000187 DADLSA010000000
VERSION     DADLSA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.188"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..231
                     /locus_tag="JN691_003730"
     CDS             <1..231
                     /locus_tag="JN691_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7245035.1"
                     /translation="IGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLISALK
                     MPEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
     gene            320..1780
                     /locus_tag="JN691_003731"
     CDS             320..1780
                     /locus_tag="JN691_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY7245036.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKTAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GNDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1816..2019)
                     /gene="ydfZ"
                     /locus_tag="JN691_003732"
     CDS             complement(1816..2019)
                     /gene="ydfZ"
                     /locus_tag="JN691_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAY7245037.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2197..2883)
                     /locus_tag="JN691_003733"
     CDS             complement(2197..2883)
                     /locus_tag="JN691_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7245038.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2972..3718)
                     /gene="ydfG"
                     /locus_tag="JN691_003734"
     CDS             complement(2972..3718)
                     /gene="ydfG"
                     /locus_tag="JN691_003734"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAY7245039.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3855..5900
                     /gene="dcp"
                     /locus_tag="JN691_003735"
     CDS             3855..5900
                     /gene="dcp"
                     /locus_tag="JN691_003735"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAY7245040.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDEFASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5945..6463)
                     /locus_tag="JN691_003736"
     CDS             complement(5945..6463)
                     /locus_tag="JN691_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HAY7245041.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1624 a         1563 c         1667 g         1691 t
ORIGIN      
        1 attggtggtc ttatcgcttc cggttttggt cctatcctag cgggtatttt ctgcaccatg
       61 acggaatcct ggtatccgat cgccattatg atcatggcat attcagtgat tggtttaatc
      121 tctgcgctga aaatgccaga ggtgaaagac cgtgatttaa gtgcgctgga agacgctgcg
      181 gaagatcaac cgcgtgttgt aagagctgcg caaccttcca gaagtctgta aaccctcaat
      241 cccttctctt accggagagg ggatttttat tcatataaca aaacatatag cttgccatat
      301 ttaaatttaa ggaattgtta tgggaaataa tttgttatca gcaaaagcga cactccctgt
      361 ttatgatcgt aataacctgg ccccaagaat tgttcattta ggctttggtg catttcaccg
      421 tgcgcatcag ggcgtgtatg ccgatattct tgctacagaa catttcagtg actggggata
      481 ttatgaggtc aacttaatcg gcggcgaaca gcaaattgcc gatttacaac agcaagataa
      541 tctttatacc gttgcggaaa tgtcggccga tgcgtggacg gctcgcgtcg ttggcgtcgt
      601 taaaaaagcc ttgcacgtac agatggatgg cttagaaacc gtgttggctg cgatgtgtga
      661 accgcaaatc gcgattgtct ctctgacaat caccgaaaaa gggtatttcc actctccggc
      721 gaccggacag ttaatgctcg atcacccgat ggtcgctgcc gacgtgcaaa atccccacca
      781 gccgaaaaca gcaacagggg tgattgttga ggcgctggct cgccgtaaaa cggcaggact
      841 tcccgcattt accgtcatgt catgtgacaa catgccagaa aacggtcatg ttatgcgtga
      901 cgttgtcact tcctacgcgc aagccgttga tgtaaaactg gcacaatgga tcgaagataa
      961 cgtgactttc ccatcaacaa tggtggaccg tattgtgccc gcagtgacag aggatacgct
     1021 ggcgaaaatc gaacaactta ccggtgtgcg cgatcctgct ggcgttgcct gtgaaccttt
     1081 ccgccagtgg gttatcgaag ataactttgt agccggacgt ccggaatggg aaaaagcggg
     1141 agccgaactg gttagtgatg tgctgcctta tgaagagatg aagttgcgca tgctcaacgg
     1201 cagtcattca ttcctggcat atctgggtta tcttgctgga tatcagcaca ttaatgactg
     1261 tatggaagat gaacattatc gtcatgcggc gtatggcttg atgttgcagg aacaagcgcc
     1321 gacgctgaaa gtgcagggcg ttgatttgca agattacgct aaccgattaa ttgcacgcta
     1381 tagcaacccg gcgttacgtc atcgaacctg gcagattgcg atggatggca gccagaaatt
     1441 gccacagcgg atgttggatt ctgttcgctg gcatctggcg catgacagca agttcgatct
     1501 gctggcgctg ggcgtcgcgg gttggatgcg ttatgtcggt ggtgttgatg aacagggaaa
     1561 tccgatagaa atcagtgacc cactgttacc tgttattcag aaggctgtac aaagtagtgc
     1621 cgaagggaaa gcgcgcgtcc agtcattgct ggcgattaag gcaatctttg gtaatgattt
     1681 gccagacaat agcttgttta ctgcaaaagt gacggaagcg tacttgtctt tattagcgca
     1741 tggcgcgaaa gcgaccgtgg cgaaatattc cgtgaagtaa aaaaagcaat gccgccatcg
     1801 gcggcattca cacacttagt tcatgccgag acgaatcagg tccagaggtg ccagtttctc
     1861 ttcacaacct tcaacaacta cggtttttcc gcggcggatt tgctcagcgg tcagaccttc
     1921 agtatcaatc gacaggatct tgccagtgtg accggtgccg ctaaccataa cacggctgcc
     1981 agtggtgatt gcgttacggt tacgatcgta ggtcgtcata ggtgttttct cctttctgat
     2041 ttacagtcga agcccacgta acgggcgcaa tataaatact cctgtcgagt gaaatatttt
     2101 ttgtttttga tcaagctcac atcttttttg atgcatgcat gagccgtaag gcggggggaa
     2161 aagcctctca tcccggatgg gatgagagta aatgaattac tcttcgctaa accagtcgct
     2221 gttttcctgg cggatctggc gcacggattc gctgatttcc tgcaaatgtt gcgtcattgc
     2281 acgttctacc gcatcgccat cacgtttttg cagggcagag aaaatatcaa gatgctggcg
     2341 taacagcatt tctggtggag aaacatggtc gaaactcata tagcgcacgc gatcaacggt
     2401 cgctttcagg ttctcaatgg tatcccacgc cagttgacag tcggcaatct gcgtcaggag
     2461 ttgatggaag ttgtcatcaa gttcaaaaaa atcatccagt tgcttgcgct caatggcaat
     2521 gcgttgctgg tgaagatttt gttccagttg gtagcactgg ctttcggtaa tcatgctcgc
     2581 cgcccgacgc gccaccgcgc actcaatggc ctgacggata aaactgccgt tgcgcacctg
     2641 ggccatggaa attttgttga cgtagctgcc acgctgcgga cgaatttgaa tcaggccgtt
     2701 ttccgccagt ttaataaagg cttcacgaac cggctggcgt gacacattga aacgaacaga
     2761 aacttctttt tccgacaacg gtgtgcctgg tgcaatcagg caatggacaa tgtcgcgacg
     2821 aagaatacga taaatctgct gattgacagg ctgtgtggga ttaagttgcg tttcgacggt
     2881 cattcgttct tgcttgtgag tgagttaact gcatgagtct accacttttt tgtgacaagc
     2941 aataacccgg cagttacgcc gggtataaaa attactgacg gtggacattc agtccggcat
     3001 agctttgggt aaccggcatc atttccaggg tattgatatt gacgtgagca ggcagcgttg
     3061 acacccacca gacggcttcg ctgacatctt ctggcgtcaa tgcaacggta ttttgatagg
     3121 ttttttccgc tttaccgtca tcgcctttaa agcggacatt ggaaaactcg gtgccaccca
     3181 ccagacccgg ttcgatgtcg gtgacgcgca ccgccgtacc atgcagatcc gtacgcagat
     3241 tcaggctaaa ctgacgaaca aacgctttcg tcgcaccgta aacgttacca ccggcatacg
     3301 gccagctgcc tgccgttgag ccaatgttaa taatatgacc atgattacgt tcaaccatac
     3361 ccggtaagac ggcgcgcgtc atatatacta ggcctttgtt gttggtatca atcatcgttt
     3421 cccagtcttc aacgctggct ttatgcgcag gctccatgcc caacgccagg ccggcattat
     3481 ttaccaggat atcaatattg ctccactcgg cagggagcga tgccagcatc tcttcaatag
     3541 cggcgcggtt gcgaacgtcc agttgggcga tatacagatt atctcccagt tcgtctttta
     3601 actcctgcaa ccgctcctgg cgacggccag tggcgataac tttatgccct tgttgaataa
     3661 aacgacgagt aatgcattca ccaaaacctg ccgttgctcc agttactaaa acgatcatct
     3721 cactgttcct caacgctttt gtgtgtcaat aacatagcac gtgatgctta agagcgggta
     3781 atactggttt gcaagattgc actccgttat cagtaagcct acgctatgga acacctcgcg
     3841 ttcaggagaa gaaaatgaca acaatgaatc ctttccttgt gcaaagcaca ctgccgtatc
     3901 tggctcctca ttttgatcaa attgccaatc atcactatcg cccggcattc gatgagggaa
     3961 tgcaacaaaa gcgggcagaa attgctgcca tcgcgcttaa cccgcaaacg cctgatttca
     4021 acaatactat tctggcactg gaacaaagcg gagaattact tacccgcgtt accagcgtct
     4081 tttttgcgat gactgcggcg cataccaatg atgaattaca gcgtcttgat gaacagtttt
     4141 ccgctgaact ggcggaactg gctaatgata tctatctgaa cggtgaatta ttcgcgcggg
     4201 tagatgctgt ctggcagcgc cgtgaatccc tggggcttga tagtgaatcc atccgcctgg
     4261 tggaggttat tcatcaacgt tttgtccttg ccggagccaa acttgcacaa gctgataaag
     4321 caaaattaaa agtactgaat acagaagctg cgaccctgac cagccagttt aaccagcggt
     4381 tacttgcagc aaataaatcc ggcggtctgg ttgtgaacga tatcgcgcag ctggcaggaa
     4441 tgagtgagca agagattgcg ctggcggcag aggcggctcg cgagaaaggt ctggataaca
     4501 aatggctgat tccgctgctg aataccaccc aacaaccggc gcttgccgaa atgcgcgatc
     4561 gtgcgacgcg tgaaaaactg tttattgcgg gctggacgcg agcggaaaaa aatgatgcca
     4621 atgatacccg cgctatcatt caacgtctgg tagagattcg cgcacagcag gcgaaactgc
     4681 ttggttttcc tcattatgcc gcatggaaaa tcgccgatca gatggcaaaa acgccagaag
     4741 cagcactcaa ctttatgcgg gaaattgttc cagcggcgcg tcaacgtgcg agcgatgaat
     4801 ttgcctccat acaggcggtt atcgataagc agcagggcgg gtttagcgcg cagccgtggg
     4861 actgggcatt ttatgcggaa caggtacggc gggagaaatt tgatcttgat gaggcgcagc
     4921 tcaagccata ttttgaatta aacacggtgt taaatgaagg tgtattctgg accgcgaatc
     4981 agctcttcgg tattaagttt gtcgaacgtt ttgatattcc ggtctaccat cctgacgttc
     5041 gtgtgtggga aatttttgat cataatggcg tgggactggc gttattttac ggtgatttct
     5101 tcgcccgtga ttcaaaaagc ggcggtgcat ggatgggcaa ttttgttgag caatcaacgc
     5161 ttaatgaaac gcatccggta atttataacg tctgcaatta tcagaaaccc gctgccggtg
     5221 agcctgcgtt gttactctgg gatgatgtaa taaccttatt ccatgaattt ggtcatacgc
     5281 tgcacggcct ttttgcccgc cagcgttatg ccacgctttc cggcaccaac acgccgcgtg
     5341 attttgtcga atttccgtcg caaatcaacg aacactgggc aacgcatccg caggtattcg
     5401 ctcgctacgc ccggcattat cagagcgggg cagcaatgcc tgacgaactg caacagaaaa
     5461 tgcgtaatgc cagcctgttc aacaaagggt atgagatgag cgaactgctt agcgccgcac
     5521 ttctcgatat gcgctggcat tgcttggaag aaaacgaagc aatgcaggat gtcgatgatt
     5581 ttgaattgcg ggcgctggtg gcggaaaata tggatcttcc tgctataccg ccacgctatc
     5641 gcagcagtta tttcgcccat atttttggtg gcggatatgc tgcaggttat tacgcttatc
     5701 tgtggacgca aatgttggcc gatgatggtt accagtggtt tgttgagcag ggcggattaa
     5761 cgcgtgaaaa tgggcagcgt tttcgcgagg cgatcctttc cagaggtaac agcgaagatc
     5821 tggaacgcct gcatcgacaa tggcgcggta aggctcctca gattatgccg atgctgcaac
     5881 atcgtggctt gaacatataa acaccaccaa tggctggcct ggagatatcg ccaggccagc
     5941 ctggttatag cagtaattgc agcagctggg cgagggcaaa cctcaccgct aaagctaata
     6001 ccgtttcgca gtcgccagca aaatgcataa ccgcagtgta gttctggcct ttaatatgcc
     6061 acgcgaacca gacggtaccc gctggcgtac catcttcacc gccctccggt ccgccgtagc
     6121 cggtaatggc aatactgaca tcagcatccg cacgctctat ggcacctgtt gccatttctg
     6181 ctgccacttt ctcactcacc gcagaatatc gttcaagaga ctgctggctt acgctgagga
     6241 ttttcatctt tgcctgatcg gtgaaagtaa caaagcctgc accgtaaaat ttgggtgtat
     6301 cttcagctgc acacagggcg ctagccaact taccgccggt gcatgattcg gcggtagtta
     6361 agcgtaattg atccgcgata agtctttgac tcaacgcgga tgtcaggttt tcaatagtgt
     6421 cggtatctgc gagttgtaca attttatctt tattaatgtt cactgttttt tgtccacgct
     6481 attatatgta aaggtgattt gctttttctg tatgctcgtc caatataata attttcaaac
     6541 atata
//
LOCUS       DADLSA010000188         6508 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.189, whole genome shotgun sequence.
ACCESSION   DADLSA010000188 DADLSA010000000
VERSION     DADLSA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.189"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..476)
                     /locus_tag="JN691_003737"
     CDS             complement(<1..476)
                     /locus_tag="JN691_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAY7245042.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYK"
     gene            665..2263
                     /locus_tag="JN691_003738"
     CDS             665..2263
                     /locus_tag="JN691_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HAY7245043.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(2245..3003)
                     /locus_tag="JN691_003739"
     CDS             complement(2245..3003)
                     /locus_tag="JN691_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAY7245044.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(3020..3850)
                     /gene="glpG"
                     /locus_tag="JN691_003740"
     CDS             complement(3020..3850)
                     /gene="glpG"
                     /locus_tag="JN691_003740"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAY7245045.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAGLARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3895..4221)
                     /gene="glpE"
                     /locus_tag="JN691_003741"
     CDS             complement(3895..4221)
                     /gene="glpE"
                     /locus_tag="JN691_003741"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAY7245046.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            4411..5916
                     /gene="glpD"
                     /locus_tag="JN691_003742"
     CDS             4411..5916
                     /gene="glpD"
                     /locus_tag="JN691_003742"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAY7245047.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(6185..6415)
                     /locus_tag="JN691_003743"
                     /pseudo
     CDS             complement(6185..6415)
                     /locus_tag="JN691_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         1578 a         1746 c         1678 g         1506 t
ORIGIN      
        1 tgtttatagt tgttagtgtt aaggaaacgc ggatattttt gcgttaacca ctgataaccg
       61 gcctccagct cagcccattt cgcatcgacg ttaaaaggcg ggttttccca tgcgccttta
      121 tcacgtttac aacgcccggt agtacgcccg tgtggtacgg ccgtttcaat cgcttgacgc
      181 agctccgcca ggttttcagg caggtctgcc gccgttaacg cggtacgcag cgcgttcatt
      241 ccacagccaa tatccacgcc caccgccgcc ggaataatcg cccctttggt cgggatcacg
      301 ctaccaatgg tggaaccttt acccaggtgt acatcaggca ttaccgcaat atgtttgaaa
      361 ataaacggca tcttcgccgt attaataagt tgctgacgcg catcggcttc taccggcacg
      421 cctttggtcc acatttttac cggggcattt tcagtggtca gtaattcgta attcattttg
      481 tttttctctt ttcgttgttt gcggtcctga taattgcaac cgtcgtgcca gaaaattaaa
      541 taagcagatt aattttttaa tttgttgttt ctaaaaagat tatatcttta tgtccgtaaa
      601 cggagatttc ccgcaaagcc gatttaccga taatgaaata tcgcctttta taaggatatc
      661 taagatgcgt aaaacagtgg cttttggctt tgtcggtacc gtgctggatt atgccggacg
      721 cggcagtcag cgctggtcaa aatggcgtcc gtcactctgt atatgccagc aagaatcgtt
      781 ggtcatcgat cgactggaat tattgcacga tgcccgctcg cgctcgctat ttgaaacgct
      841 taaacgcgat atcgccagcg tttcgccaga aacagaagtg gtgggcgttg agattgaact
      901 gcataacccg tgggatttcg aagaggttta cgcctgcctg catgatttcg cccgtggtta
      961 cgagtttcag ccagaaaaag aagactattt aattcacatc accaccggca cccacgtcgc
     1021 gcagatttgc tggtttctgc tggcggaagc gcgttatcta cccgcccggt tgatacaatc
     1081 ttcaactccg cgcaaaaaag aacagccgcg cggcccaggt gaagtgacga ttatcgatct
     1141 cgatttaagc cgttataacg ccatcgccag ccgctttgcc gaggaacggc agcaaacgct
     1201 cgatttcctc aaatccggta ttgccacgcg taacccccac ttcaaccgca tgattgagca
     1261 gatcgaaaaa gtggcgatta aatcccgcgc gccgattctg cttaacggcc cgaccggcgc
     1321 aggtaagtca tttctggcgc gacgcatctt cgagttaaaa caggcacggc atcagtttag
     1381 cggcgcgttt gtggaagtga actgcgccac cctgcgcggc gataccgcga tgtcgacgct
     1441 gtttggtcat gtgaaaggcg cgtttaccgg ggcgcgggaa tctcgtgaag gattattacg
     1501 cagcgccaac ggcggaatgt tgtttcttga tgagattggc gaactgggcg cagacgaaca
     1561 ggcaatgctg ctgaaagcta ttgaagagaa aaccttttac ccgtttggca gcgatcgcca
     1621 ggtgagcagt gattttcagc ttatcgccgg aacggtgcgc gatttgcgcc agctggttgc
     1681 cgaaggcaaa ttccgcgaag atctgtacgc gcggatcaat ctctggacct tcaccctgcc
     1741 gggtctacgc cagcgccagg aagatattga accgaacctg gattatgaag tggagcgcca
     1801 cgccacactc actggtgaca gcgtgcgttt taacaccgaa gcgcggcgtg cctggctggc
     1861 ctttgcaaca tctccccagg caacatggcg cggtaacttt cgcgaacttt ctgccagcgt
     1921 cacgcggatg gccacctttg ccactagcgg acgcatcact ctggacgtgg tggaagatga
     1981 gataaaccgt ctgcgctata actggcagga gagccgcccc tccgcgctta cggcgttgct
     2041 gggcgcagag gcagaaaaca tcgatctctt cgaccgtatg caactggaac acgttatcgc
     2101 tatctgccgc caggcaaagt cgctttctgc tgccggacga cagctttttg atgtttcgcg
     2161 ccagggcaaa gccagcgtca atgacgcgga tcggctacgc aaatacctgg cgcgttttgg
     2221 tctgacgtgg gaagccgtgc aggatcagca cagctccagt tgaatatggt ggtccgtcag
     2281 cacctgcatc acgcttactg gcggcggggc gtcggtgtag acggcatcaa ccatgctgat
     2341 gctccccata ttgaccatcg cattacggcc aaattttgaa tggtcaacca ccagcataac
     2401 gtggcgcgag ttctcaataa tggcgcgctt ggtacgaact tcgtggtaat cgaactccag
     2461 cagcgagccg tcgctgtcga tgccgctgat ccccagaatg ccgaaatcaa ggcggaactg
     2521 ggagataaaa tcgagcgtcg cttcgccaat gatcccgcca tcgcggctac gtaattcgcc
     2581 accggcgaga ataatgcgga aatcttcttt taccatcaac gtgttagcaa cgttgagatt
     2641 gttggtgaca atgcgcaaat tgctgtgatt gagcagcgcg tgcgccaccg cttccggcgt
     2701 ggtgccgata tcgataaaca gcgtcgagcc attggggatt tgctccgcca ctttgcgggc
     2761 gatgcgctct ttttcttcgg tctgggtggc tttacgatcg tgccacggcg tgttaaccga
     2821 actggaaggc agcgccgcgc cgccatgatg gcgcaggatc aggttttgct ctgccagctc
     2881 gttgaggtcg cggcgaatag tctgcgggct gacggagaag tgctccacca gttcctcggt
     2941 actgacataa ccctgctgtt taaccagttc gataataccg ttgtgacgtt gtgtttgttt
     3001 catttataaa tccctggaat tattttcgtt ttcgcgcatt gagcgaatca acaaaagcca
     3061 tcgctaaacc cacggctaat ccggcgatgt gtgctccgtt cgccatcgac atcccaaaca
     3121 aatcaaacca tccggcgaca atccagatca gcgcaaagat aattaaccca cgttgcaggt
     3181 aaatgccact ttgcggatcg cgttcgccac gtagccagac gtagcccatc agcgcataca
     3241 ccacgccaga aagcccgcca aaccacggcc cgctgaattt ttgctgcaca tagccgctta
     3301 acagggcgct gataagcgta atgacaatta gcttaccgct accgaggcgt ttttccaccg
     3361 cgccgccgag ataccaccac caaagcaggt taaagaggat atgcatcagc gagaagtgca
     3421 ttaacgcgtg ggtgaagtaa cgccagaact caaatttcag tgttggatcg aatggccagg
     3481 ccagccataa catcacttcc tgatcgccga gaatttgcat ggcaataaac accaccacgc
     3541 aggcgatcat catcacccag gtcaccggac ctgcgcgttc acgcaaggca gcaaagaaag
     3601 gataacggcg ataatgcagg ccactgccgg tatggcctgc cagccagctg gccgccagat
     3661 aacgcggatc tgccgggttt tcgagaaaac gcgccagccc cgcccgcacg cgctcggcct
     3721 gggactcatc cgccagccag acatcgcttt ggttatgttg ttgaatcgtg aggataacac
     3781 cctgcgtcgc catgtaatca acaaacgcct gcgccacgcg ggggttagca aaagaggtaa
     3841 tcatcaacat cgttgctgtc gcttattcca cacaaaaggg gacagtataa agcgttacgc
     3901 gccgtacgcc acctctgcgg gaaactgtcg ctgccaggct tcaaagccgc cgtcaatgct
     3961 atagaccaca tcgtagccct gttgcagcag atactgcgcc gcccctttgc tgctattgcc
     4021 gtgataacac atcaccatca ccggagtgtc aaagtcgtta tcacgcataa acgcgcccag
     4081 cgtgtcgttg gttaaatgga aagcctgcac cgcatgcccc atagcgaaac tctgtggatc
     4141 gcgaatatcg accagcaccg cctctttttc ctgcaacttc tggtgcgcgt cggcaacgtt
     4201 aatacattcg aactgatcca tgcgtctctc tttctttaca aacaagtggg caaatttacc
     4261 gcacagttta cgtcgaagcg gcagataaac gccataatgt tatacatatc actctaaaat
     4321 gttttttcaa tgttacccaa agcgcgattc tttgctaata tgttcgataa cgaacattta
     4381 tgagctttaa cgaaagtgaa tgagggcagc atggaaacca aagatctgat tgtgataggt
     4441 ggcggcatca atggtgctgg tatcgcggca gacgccgctg gacgcggttt atccgtgctg
     4501 atgctggagg cgcaggatct cgcttgcgcg acctcttccg ccagttcaaa actcattcac
     4561 ggtggcctgc gctaccttga gcactatgaa ttccgcctgg tcagcgaggc gctggctgaa
     4621 cgtgaagtgc tgctgaaaat ggctccgcat atcgccttcc cgatgcgttt tcgcctgcca
     4681 catcgtccgc atctgcgccc ggcgtggatg attcgcattg gtctgtttat gtacgatcat
     4741 ctgggtaaac gcaccagcct gccgggatca actggtttgc gttttggcgc aaattcagtg
     4801 ttaaaaccgg aaattaagcg cggattcgaa tattctgact gttgggtaga cgacgcccgt
     4861 ctggtactcg ccaacgccca gatggtggtg cgtaaaggcg gcgaagtgct tactcggact
     4921 cgcgccacct ctgctcgccg cgaaaacggc ctgtggattg tggaagcgga agatatcgat
     4981 accggcaaaa aatatagctg gcaagcgcgc ggcctagtta acgccaccgg cccgtgggtg
     5041 aaacagttct tcgacgaggg gatgcatctg ccttcgcctt atggcattcg cctgatcaaa
     5101 ggcagccata ttgtggtgcc gcgcgtgcat acccaaaagc aggcctacat tctgcaaaac
     5161 gaagataaac gcattgtgtt cgtgatcccg tggatggacg agttttccat catcggcacc
     5221 accgatgtgg aatacaaagg tgatccgaaa gcagtgaaga ttgaagagag tgaaatcaat
     5281 tacctgctga aagtgtataa cgcgcacttt aaaaagcagc taagccgtga cgatatcgtc
     5341 tggacctact ccggtgtgcg tccgctgtgt gatgatgagt ccgactcgcc gcaagccatt
     5401 acccgtgatt acacccttga tatccatgat gaaaacggca aagcaccgct gctgtcggta
     5461 ttcggcggta agctgaccac ctaccgaaaa ctggcagaac atgcgctgga aaaactgacg
     5521 ccgtattatc agggtattgg cccggcgtgg acgaaagaga gcgtgctgcc gggtggtgcc
     5581 attgaaggcg accgcgacga ttacgccgct cgcctgcgcc gccgttaccc gttcctgact
     5641 gaatcgctgg cacgtcacta cgcgcgcact tacggcagca acagcgagct gctgctcggc
     5701 aatgcgggaa cggtaagcga tctcggggaa gatttcggtc atgagttcta cgaagcggaa
     5761 ctgaaatacc tggtggatca cgaatgggtc cgccgcaccg acgatgccct gtggcgtcgc
     5821 acaaaacaag gcatgtggct gaatgcggat caacaatctc gtgtgagtca gtggctggtg
     5881 gaatatacgc agcagaagtt gtcgctggcg tcgtaaatta acgtaaggtg atcaggtcag
     5941 atttcaatct ggcctgagac tgatgacaaa cacaaaactg cctgatgcgc tacgcttatc
     6001 aggcctacgt ggtttatgca atatattgaa tttgcatggt cttgtaggcc agataagacg
     6061 ttcacgtcgc atccggcatg aacaaagcgc actttgtcaa aaatctaacc tactttttaa
     6121 ttcagggaat taccgcaaag cccacgccat catacccaac gtaacaaaac tcaggcacgt
     6181 tccccactcg ccccgagaaa aatagcatta atgcgcccag cgtcagcata aaaatattga
     6241 gcagaaggta aattgttggc gtaaagagta aattgcaaac ctcaggaata gcggcaagcc
     6301 cagcccccag catggcaata accgctgcaa aaaattgcgt cagtattggg atgccgaaaa
     6361 aagtcattac cagcgaggtc aaaatccatt ttctattttg cattattctt tccattcttt
     6421 ctgaatggcg aaattatact ccccgagtcc ccttgcccct tctggacact tttccgaaat
     6481 gatggcgaaa aaaacgggac cctttggc
//
LOCUS       DADLSA010000189         6493 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.190, whole genome shotgun sequence.
ACCESSION   DADLSA010000189 DADLSA010000000
VERSION     DADLSA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.190"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            60..473
                     /locus_tag="JN691_003744"
     CDS             60..473
                     /locus_tag="JN691_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001945043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7245048.1"
                     /translation="MAEQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVA
                     FSCKRRGFCPSCGARRMAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFGVVCGKGRN
                     PTLRLWPAIFSGEIDVFPGDRRSRLISSMTSVAPA"
     gene            complement(445..1281)
                     /locus_tag="JN691_003745"
     CDS             complement(445..1281)
                     /locus_tag="JN691_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAY7245049.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(1281..2084)
                     /locus_tag="JN691_003746"
                     /note="aph(3'')-Ib"
     CDS             complement(1281..2084)
                     /locus_tag="JN691_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAY7245050.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(2145..2960)
                     /gene="sul2"
                     /locus_tag="JN691_003747"
     CDS             complement(2145..2960)
                     /gene="sul2"
                     /locus_tag="JN691_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAY7245051.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2984..3061
                     /locus_tag="JN691_003748"
                     /pseudo
     CDS             2984..3061
                     /locus_tag="JN691_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            3290..3466
                     /locus_tag="JN691_003749"
     CDS             3290..3466
                     /locus_tag="JN691_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HAY7245052.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            complement(3648..4652)
                     /locus_tag="JN691_003750"
     CDS             complement(3648..4652)
                     /locus_tag="JN691_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HAY7245053.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTLLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
     gene            complement(4731..>6493)
                     /locus_tag="JN691_003751"
     CDS             complement(4731..>6493)
                     /locus_tag="JN691_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAY7245054.1"
                     /translation="RAAPAAKDVLDAIEVLRGMNSDNARKVPADAPTEFIKPRWQKLV
                     MTDTGIDRRYYELCALSEMKNALRSGDIWVQGSRQFKDFEDYLVPPAKFASLKQASEL
                     PLAVATDCNRYLNDRLTLLETQLATVNRMATANELPDAIITESGLKITPLDAAVPDTA
                     QALIDQTAMILPHVKITELLLEVDEWTGFTRHFAHLKSGDPAKDKNLLLTTILADAIN
                     LGLTKMAESCPGTTYAKLAWLQAWHIRDETYGAALADLVNAQFRHPFAEHWGDGTTSS
                     SDGQNFRTGSKAESTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGVRDSTYVLD
                     GLLYHESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYD
                     ALKPMIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRE
                     LGRIERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRY
                     RASGLNLVTAAVVLWNTVYLERAAHALRGNGHAVDDALLQYLSPLGWEHINLTGDYLW
                     RSSAKIGAGKFRPLRPLQPA"
BASE COUNT         1402 a         1809 c         1872 g         1410 t
ORIGIN      
        1 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
       61 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
      121 aatgcgggcg gctggagcat ggctttctac gggttcgctg cgagtcttgc cacgccgagc
      181 acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga
      241 tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt
      301 gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc
      361 ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat gtgttcccag
      421 gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct tgatcgcggc
      481 cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg cggaaaggca
      541 cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg cacgagagaa
      601 tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc tgtcagccgg
      661 atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga cgggatctat
      721 caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc catgtagccc
      781 acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt ggacgtagtc
      841 agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg caaagcgatc
      901 ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca gcttcgccat
      961 tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga tgtgagagag
     1021 cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga gcaaccggac
     1081 tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat cagcaatgtc
     1141 ttctataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg aaactttcca
     1201 aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc aatgagcagg
     1261 aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag atagaaggca
     1321 aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc gaaggcgcgc
     1381 tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag tgccaaatcg
     1441 gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca ttgaagagtt
     1501 ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac catatcggtg
     1561 cgctcttggt cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc
     1621 ggcgtactct tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca
     1681 tcaacggcgc gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat
     1741 aggctgtgaa cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct
     1801 ccagacagat cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc
     1861 tgttcctcct gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg
     1921 aggcggtcac gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag
     1981 gcatgcccgt caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac
     2041 cagtcagaat gcgattcacc aaaaaaaata ttagttcgat tcaatggagg ttccttcagt
     2101 tttctgatga agcgcgaata tagagaaata tcccgaatgt gcagttaacg aattcttgcg
     2161 gtttctttca gcgccgccaa taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg
     2221 cggatgaagt cagctccacc tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg
     2281 acatcccccg gaccacggcc tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga
     2341 agcaccggca aatcgaagcg cagccgcaat tcatcgaacc gcgccagcac cgagagcgag
     2401 gtttcgggag cagcccccag aaaaaacccc atgccgggat caaggacaag gcggttgcgt
     2461 ttgataccgg cacccgtcag cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc
     2521 atgatgtcgc cagcgggtgc ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata
     2581 acgacgagtt tggcagatga tttcgccaat tgcggataga acgcagcgtc tggaaaaccg
     2641 cgaatatcat tgagataggc cacaccacgc gacaaggcat aggcttgcgt cgcgggttga
     2701 taactgtcga gcgagacggg aatgccatct gccttgagcg cgtccagcac cggcgcgata
     2761 cgcgcgattt ctgtgtcgga cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg
     2821 aggtcgatca catctgcccc ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct
     2881 ggcgccagat accggcctcc atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa
     2941 atgatgagcg atttattcat gggggcttct ataataataa taatcgagca tgagtctcat
     3001 acggatgctc gggtcgaaag ggaatcccca ggcgagtaac ctgtttgcgg tgctccatta
     3061 gctgcaggag cagaatagca tacatctgga agcaaagcca ggaaagcggc ctatggagct
     3121 gtgcggcagc gctcagtagg caatttttca aaatattgtt aagccttttc tgagcatggt
     3181 atttttcatg gtattaccaa ttagcaggaa aataagccat tgaatataaa agataaaaat
     3241 gtcttgttta caatagagtg ggtatcggag acgaagaagg acaaggtaca tgctgaccga
     3301 taccaagctg cgcaatctca agcccaggga caaactctac aaagtgaatg accgggaagg
     3361 tctctatgtg ggggtcgcct cagaaaacgg aatctatggt cactcccgtt tttgcaacac
     3421 cgattttgac gacaagttgg cttgcttgaa tctatccggc gtctgaatgg gattttattc
     3481 ccgcgcctcg atgagttccg cgcctgatga acctccagaa aatatacggc ttcaatgagc
     3541 ctttccgttt tacaggttcc tcaacaggcc ggtgggccgt tagtatcatc aatatcagta
     3601 ttcgcaaaac cagatgaatg attgtttaaa ctggtgtatt tctgccttta tgcttcgtaa
     3661 gtttgctgtc gcgccgtcag tgcccaggct attctggcca gcttgtttgc cagagcacag
     3721 gtgacgacaa agttgctttt ccgacacaac aactccctga cccagtcggc caacttgcca
     3781 gactggtgtt ccagtttttg tatgaatacc ctggcacact gaaccaacaa agttcggatc
     3841 tttttgttgc cccgcttgct aatccctaac aatgtcgtcc gacctcccgt gctgtactgt
     3901 cggggtacca gccctgttgc cgccgcaaag tcacggctgc tggcgtactg cttcccgtcg
     3961 ccaatctcag ttgaaatagt actggcagtc agcgttccaa cgcagggaat actcagcaag
     4021 cgctgtccaa cctcatcttc gtccaacttt cgtttcaact gagattccag atctttaatc
     4081 tgctcaacaa gatagtgata atgctgttgt aatttcagca gtaactggct gagataaaga
     4141 ggcaaactac tgtcctcaag aagggtactc agtcgactaa taacggcagc acctcgcgga
     4201 acgctgatac caaattccag cagaaaagca tgcatctgat tagttgtttt caccttatcc
     4261 tgaaccaggg attcacggac acgatgcaga gctcgcattg cctgctgaga ttcggttctg
     4321 ggctgcacga aacgcataga tggacgtgat gctgcttcac agatagcttc agcatcaacg
     4381 aagtcatttt tgttgctttt aacgaatggg cggacaaatt gcggtgatat cagctttgga
     4441 aaatgcccta actcttccag cttgcgtgcc ataaagtgag aaccgccaca ggcttccatc
     4501 gcgatggttg ttgccgggca tgtcgccaga aattcgatta gctttggtcg ggtgaatttt
     4561 ttacggtaaa cggccttccc acgatgatcc tgacaatgaa tatggaaaga gttcttaccc
     4621 agatcgatac caataagcgc aatgttttcc atgatggttc tccgaatgaa agcctgtcct
     4681 cagcatagta ctgggaagga gggagtgacc atctcattaa ataaagcacg ctaagccggt
     4741 tgcagcggtc gtagcggcct gaacttgccc gcgccgatct tggcgctgct gcgccagagg
     4801 taatcgccgg tgaggttgat gtgctcccaa ccgagcggcg acaggtactg caacagcgcg
     4861 tcatcaacgg catggccgtt gccacgcagc gcgtgcgcag cccgttccag atagaccgtg
     4921 ttccacaaca cgacggcagc cgttaccaga ttgaggccgc tagcccggta gcgctgctgc
     4981 tcgaaactgc ggtcgcggat ttcacccagg cggttgaaaa acactgccct ggccagcgca
     5041 ttgcgcgcct cgcccttgtt caggccggca tgcacgcggc ggcgcagttc cacgctttgc
     5101 agccagtcca ggatgaacag cgtgcgctcg atgcggccca gctcgcggag cgccacggcc
     5161 aggccgttct ggcgtgggta gctgccgagc tttcggagca tcagggaggc cgtcaccgtg
     5221 ccctgcttga tcgaggtggc cagccgcagg atttcgtccc aatgggcgcg gacgtgcttg
     5281 atgttgagcg tgccgccgat catgggtttc agcgcgtcat aggcggcgtc gcccttcggg
     5341 atgtagagct tggtgtcgcc caggtcgcgg atgcgcggcg cgaagcggaa gcccaggagg
     5401 tgcatcaggg cgaagacgtg atcggtgaag cccgccgtgt cggtgtaatg ctcctcgatc
     5461 cgcaagtcgg actcgtggta cagcaggccg tcgagcacgt aggtcgaatc gcgcacgccg
     5521 acgttcacga ccttggtgtg aaatggcgcg tactggtcag aaatgtgggt gtagaacgtc
     5581 cgccctgggc tgctcccgta tttcgggttg atgtggccgg tgctctcggc cttgctgccg
     5641 gtgcggaagt tctggccgtc cgacgatgag gtggtgccgt cgccccagtg ctcggcgaag
     5701 ggatggcgga actgtgcgtt gaccagatcg gccagcgccg ccccgtaggt ttcgtcgcgg
     5761 atgtgccagg cttgcagcca agccagcttg gcgtaggtcg tgccggggca agactccgcc
     5821 atcttggtca ggcccaggtt gatcgcgtcg gcgaggatcg tggtcagcaa caggttcttg
     5881 tctttggccg ggtcgcccga tttcagatgc gcgaaatgcc gagtgaagcc cgtccattcg
     5941 tccacctcca gcagcagttc ggtgatcttg acgtgcggca ggatcattgc cgtctggtcg
     6001 atcagcgctt gggcggtgtc gggtaccgcc gcgtcgagcg gcgtgatctt caagcctgac
     6061 tcggtgatga tggcgtccgg cagctcgttg gccgtcgcca tacggttgac ggtggcaagc
     6121 tgtgtttcca gcagcgtcag ccggtcgttc aggtaccggt tgcagtcggt ggccacggcc
     6181 agcggcaatt cgctggcctg cttgaggctg gcgaatttcg cgggcggcac caggtagtcc
     6241 tcgaagtcct tgaactggcg cgacccctgc acccagatgt cgccggaacg caacgcgttc
     6301 ttcatctccg acagcgcgca cagttcgtag tagcgccggt cgatgccggt gtcggtcatg
     6361 accagcttct gccagcgcgg cttgatgaac tcggtcggcg cgtcggcggg caccttgcgg
     6421 gcgttgtcgc tgttcatgcc gcgcagcacc tcgatggcgt cgagcacgtc cttcgcggcg
     6481 ggagcggccc gca
//
LOCUS       DADLSA010000190         6268 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.191, whole genome shotgun sequence.
ACCESSION   DADLSA010000190 DADLSA010000000
VERSION     DADLSA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.191"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="JN691_003752"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="JN691_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HAY7245055.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="JN691_003753"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="JN691_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAY7245056.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /locus_tag="JN691_003754"
     CDS             complement(2659..5772)
                     /locus_tag="JN691_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY7245057.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLAAGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5827..6054)
                     /locus_tag="JN691_003755"
                     /pseudo
     CDS             complement(5827..6054)
                     /locus_tag="JN691_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
BASE COUNT         1661 a         1459 c         1482 g         1666 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgatctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctggcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagcaaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctag ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag atgatgtgat tacaggtt
//
LOCUS       DADLSA010000191         6178 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.192, whole genome shotgun sequence.
ACCESSION   DADLSA010000191 DADLSA010000000
VERSION     DADLSA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.192"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            9..161
                     /locus_tag="JN691_003756"
                     /pseudo
     CDS             9..161
                     /locus_tag="JN691_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1542..2489
                     /gene="icsP"
                     /locus_tag="JN691_003757"
     CDS             1542..2489
                     /gene="icsP"
                     /locus_tag="JN691_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HAY7245058.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQAETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3050..3422)
                     /locus_tag="JN691_003758"
                     /pseudo
     CDS             complement(3050..3422)
                     /locus_tag="JN691_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3425..3610
                     /locus_tag="JN691_003759"
                     /pseudo
     CDS             3425..3610
                     /locus_tag="JN691_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3653..3772
                     /locus_tag="JN691_003760"
                     /pseudo
     CDS             3653..3772
                     /locus_tag="JN691_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4022..4888
                     /gene="ospB"
                     /locus_tag="JN691_003761"
     CDS             4022..4888
                     /gene="ospB"
                     /locus_tag="JN691_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HAY7245059.1"
                     /translation="MNLDGVRPYCRIGNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5217..5957
                     /locus_tag="JN691_003762"
     CDS             5217..5957
                     /locus_tag="JN691_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY7245060.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
BASE COUNT         1941 a         1158 c         1240 g         1839 t
ORIGIN      
        1 gttcctaagt ggaatgtctc cacggagaac actttatcta tcgggaaata gtgcgggcaa
       61 cagtgtttaa ttatatcgag tgcgattaca atcgctggcg acggcaccgt tggtgtggcg
      121 gcctcagtcc ggaacaattt gaaaaccaga acctcgctta ggcctgtgtc catattacgt
      181 gggtaggctc acagagcctc acttacgact gcgtccatat tatgtgggta ggatcaataa
      241 agacgtttta tatgtgtctg cttgcagcca gaaaatttat accctgcagg tatcataaac
      301 atgcttgata acttaattgg ggctccctct tccttttatg gtgtaattgt ttgattgaat
      361 acttccgggg atttcagtat gaaatgaagt atatttaata tactttacaa aaagtttgta
      421 ttagtggaat aaggacaata gcttatccaa actgccaagc ctctttatta taagtaagat
      481 ctggcactgg aatattctca aaaggactaa catcatcacc acgaccttca accaaccatt
      541 ttattgcatt taaatcccag aatagttcag tagtggtcaa tttgggaaac gcacaatatt
      601 gtacggttac gttatcgcaa tagagtgaac tcccgcccta tacggcgttt tcaaggatta
      661 gtccttaatc ggacaaccac attaaagcat aataaataat taagctttaa taatatacta
      721 tagatacagt tctattataa aaaatactat tttaggtgat gcattaaatt agtagaatat
      781 ttattctttt ataaaagcac caatgaattg tccaagtaaa catgaggtaa aatgtgtaga
      841 ggataactga gtttggtcac tttaactgta ttagtcgcag tctgactctc gactttaaaa
      901 ggatgggaat tacctgtact gatagtgaga aagctttcca ttgaaataag tcaaataaaa
      961 gttgatggct tcagtgctaa agtgaggtgc ccgctagccg tattcactcg aatcggaatt
     1021 cgatgtattg cttctgccat ttccataata tttcacggga aaaacaaaat ctgaaaaact
     1081 atacttaagt gttgtattga gtcagttgag atacaaatac atatttatca gcacacgtta
     1141 cgtcaaatac aggaggttgt catgttggta ctgaaaggca cgttggctat tgcagccgtg
     1201 atgacaattt attgtcttat cgttgttctt atggaacgct tttctgattg atttcatatt
     1261 ggcgagagaa caagaaggtg agtagaattg tcgcggaatc ctgaaactat cagcctcagg
     1321 tatgaacacc aaaaggtagg tagagaaatc tatggccccc gttactttat tcgcacaatg
     1381 aggtattttt ttatttttac aaaagttcaa taaatattat aataaaagat atgttcttgg
     1441 taacataaac aaatatttaa ttaaaataat gcttggcatt gacgtttatc ttataaagta
     1501 agaagatcat ggacatttca accaaaaaag tagagttctc gatgaaatta aaattccttg
     1561 tacttgcact ttgtgtacct gcgatcttta ctacacatgc taccactaac tatccacttt
     1621 tcataccgga caacatcagt acggatatta gtctaggatc tctgagtggc aaaacaaaag
     1681 aacgcgttta tcatcccaag gaagggggga gaaaaattag tcaactggac tggaaataca
     1741 gtaatgcgac tattgttaga ggtggcatcg attggaagct aattccaaaa gtgtctttcg
     1801 gagtttccgg ttggactact ttaggtaacc agaaagcaag catggttgat aaagactgga
     1861 acaattccaa tacccctcag gtatggaccg accagagctg gcatcccaat acgcatctcc
     1921 gtgatgctaa cgaattcgag ctgaatctta aaggttggtt attaaataat ttggattatc
     1981 gacttggact aatagcaggt taccaagaga gtcgttacag ttttaatgca atgggaggga
     2041 gttatattta tagtgaaaac ggggggaaca ggaataaaaa aggggcacat cctagtggtg
     2101 aaagaacaat aggttacaaa cagctcttca aaatacctta tattggatta actgctaatt
     2161 accgccatga gaattttgag tttggagcag aactgaaata tagtggttgg gttcgttcat
     2221 ctgatacaga caaacactat caggctgaga caatttttaa agatgaaata aaaaaccaaa
     2281 attactgctc tgttgctgcg aatattggat actatgtcac ccccagtgca aaattttata
     2341 tagaaggctc cagaaattac atttctaata aaaaaggtga tacatctctt tatgagcaaa
     2401 gtaccaatat atctggcacc attaaaaata gtgcaagtat tgaatatatt ggttttctca
     2461 cttccgcagg tataaagtat attttttgaa ttggttcacc gagttgatta cgttttcatg
     2521 gctaaaaatt gccgcccatt gtgagtcata ttcgccctga ctttccagaa ccatacgaac
     2581 tgcccgttga cggacttcgg gggaaaaacg tgtattttta gtcattctgt ttacctcttt
     2641 ctcagggcgt ttagtctcca ggatttccgg ggcggttcaa aaaactcttc gttgattact
     2701 gtggacttac tgttggcatt accgaccctg agaccggaga aataagaacc gcttagatca
     2761 tcgtggctgt tctcggggta tcaagttaca catgggcaga gcctacccaa ggtctactat
     2821 tatcgcctga aagcgctgat ggaacagtgc tatcagggac atgctgatga aaatacctaa
     2881 ataatcagcc agcactctat ctttccaaat ccacagcata gcaaagagag caaaagagcc
     2941 tgtaaattca gaaattaagg aaagcctctt ggtgcacata atctatctta tgttaaatga
     3001 aatctaattt cttgattata tgctggatcg ttcgtgggtg gctttgcccc tatatttcca
     3061 gacacctgtt atcattggcc cgctgccgca gatattcccg tggcgagcga taatccagcg
     3121 cgctatgcgg atgccattcg ttataatgct cgaatgcctc tgcaaggttc tttgctgccg
     3181 ttaacccgtc aggtttgggc atgatactga tgtagtcaca ctttattgtt ttcacgaagc
     3241 tctttgctat tctgttactc tccggactcc gcaccgccgt gttcttcggt tcaacaaagt
     3301 cccaacatcc gggtgaactg gcgtgtttca ttagcccggt agcatgaacc attatccgtc
     3361 agtcacttca ctggagacgc cggaagctcg ttgccgaagc ggtgttccac cgctcccagc
     3421 atgtcgccaa cgggagagca tctacggtct gattgaactg tttattcaaa ttgtccaccg
     3481 tctgtcagta cgctctgaac gtcggctggt taaaacactg ctcgctgatt ttcagagggt
     3541 tcacggtaaa acagcacttt tattccggat tgcggaagcg gctctgaata atccggatgg
     3601 gctggttaaa atctgcatat tgttgaacca gatcgcagcg gtaagagaag ttcatcatac
     3661 ttggcgcagt tacgcgatgt ttctgctaga ggtgatgccg taaagaacgg cagaacatta
     3721 ccggaacaag atagcggtct acctgcactg gtatcagacc cggggcttcc ccggatgatc
     3781 tccacggtac tttaggtact gtgtctatat tatgtaggta ggatcaatta actttgtagt
     3841 tgaaattctt tcattgaaat aatgaataaa aaagacaaac cgcattaaac aatgccagaa
     3901 tataattgca tttttgttcc gtttttttaa atcatagtat acaaagaagc ttgcatacta
     3961 tatgaaaata taacatcctt tactatgatg tttataaaac aatatatgga gtcatgtagg
     4021 tatgaattta gatggtgtta gaccatactg tagaataggc aataaaaaga atgaaagcat
     4081 atcagatatt gcatttgcac atataataaa aagggtaaaa aattcatcat gtactcaccc
     4141 aaaagcagca ttggtttttt taggagagaa aggtttttgt gatagcaatg atgttctatc
     4201 tataatggga caacaaatac caagagtatt taagaacaag atgttatatg attatgtttt
     4261 taaaaatgaa aaaagtaaaa atgattttct aaaaatggct gaatcatggc taccacagag
     4321 tgaaccaata gtaataaata atgatgatga cgcattgaat gctgctgctt atttttctgt
     4381 aaaaaaagcg aaaataaaaa cagtaaacga tactgatttt aaagagtata ataaggttta
     4441 tattcttggg cacggtagtc ctggttctca tcaattaggc cttggttcgg aacttattga
     4501 tgtacaaaca atcatttcaa gaatgaaaga ctgtggtatt ctaaatgtga aagatatccg
     4561 ttttacttca tgcggctccg ctgataaagt ggctcctaaa aattttaaca atgcccctgc
     4621 tgaaagtctt tcttgtatcc ttaactctct gccttttttt aaggaaaaag aatctttgct
     4681 agagcagata aaaaaacacc ttgaaaacga tgagtcattg agtgatggtc taaaaatatc
     4741 cggctatcat ggatatggag ttcactatgg tcaagagctt tttccctact cacattatcg
     4801 ttcaacttca attcctgctg atccggagca tacagtaaaa agaagctctc agaaaaagac
     4861 ttttattatt aataaagaac tggattagta taaaattttt aacctatagt taattgtttg
     4921 catttaagat gaagttattt tacctttagt agaaaagtgc ttccctatga gggaaaatca
     4981 caaatcatca taatacagag acaaaacgat acgaacaaat agataaaaaa catcattctt
     5041 ttaccacatt atttttcgtg aatatgaaaa ataatgttat tactttaata taagactatt
     5101 ttttgttttt ccatcactct gttcaaattt ttccgcatga cttgtatttt ttgtaacaca
     5161 gctcgttttt tacagctgac caaaatcatc aattaattat gctaaggaaa taaattatga
     5221 aaaccaaaaa ctttcttctt ttttgtattg ctacaaatat gatttttatc ccctcagcaa
     5281 atgctctgaa ggcagaaggt tttctcactc aacaaacttc accagacagt ttgtcaatac
     5341 ttccgccgcc tccggcagag gattcagtag tatttcaggc tgacaaagct cattatgaat
     5401 tcggccgctc gctccgggat gctaatcgtg tacgtctcgc tagcgaagat gcatactacg
     5461 agaattttgg tcttgcattt tcagatgctt atggcatgga tatttcaagg gaaaataccc
     5521 caatcttata tcagttgtta acacaagtac tacaggatag ccatgattac gccgtgcgta
     5581 acgccaaaga atattataaa agagttcgtc cattcgttat ttataaagac gcaacctgta
     5641 cacctgataa agatgagaaa atggctatca ctggctctta tccctctggt catgcatcct
     5701 ttggttgggc agtagcactg atacttgcgg agattaatcc tcaacgtaaa gcggaaatac
     5761 ttcgacgtgg atatgagttt ggagaaagtc gggtcatctg cggtgcgcat tggcaaagcg
     5821 atgtagaggc tgggcgttta atgggagcat cggttgttgc agtacttcat aatacacctg
     5881 aatttaccaa aagccttagc gaagccaaaa aagagtttga agaattaaat actcctacca
     5941 atgaactgac cccataaagc tggacagcct gtatcaggct atggagggcc catagacaaa
     6001 tctaccctat atgagcatag gaggagtcta tgggcacacc acgttttacc cttgaattta
     6061 agggattact ggaaaggctg ggacatatcc ctccggcaga agcagaaaaa gcttattatg
     6121 ctgccatcgg aaacgatgat ctggcaacct gagttcacag ataaaacatt ctctagga
//
LOCUS       DADLSA010000192         6126 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.193, whole genome shotgun sequence.
ACCESSION   DADLSA010000192 DADLSA010000000
VERSION     DADLSA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.193"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            186..513
                     /locus_tag="JN691_003763"
                     /pseudo
     CDS             186..513
                     /locus_tag="JN691_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(543..3455)
                     /locus_tag="JN691_003764"
     CDS             complement(543..3455)
                     /locus_tag="JN691_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245061.1"
                     /translation="MFSKAFLRKISMFFARRKQAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTRLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3458..3574
                     /locus_tag="JN691_003765"
     CDS             3458..3574
                     /locus_tag="JN691_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7245062.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3938..4780
                     /locus_tag="JN691_003766"
     CDS             3938..4780
                     /locus_tag="JN691_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY7245063.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAEGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4783..5871
                     /gene="capU"
                     /locus_tag="JN691_003767"
     CDS             4783..5871
                     /gene="capU"
                     /locus_tag="JN691_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY7245064.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5876..>6126
                     /gene="virK"
                     /locus_tag="JN691_003768"
     CDS             5876..>6126
                     /gene="virK"
                     /locus_tag="JN691_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY7245065.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGR"
BASE COUNT         1618 a         1433 c         1468 g         1607 t
ORIGIN      
        1 ggccgccggg gacgtttttt taccaccggg agtttgggca taacggtgct ttcagtaagt
       61 acgacaggta ctgtggaggc acttccgtaa agaagggcat caagctcctg ctccgacata
      121 cctgcaggca aaggccatga aagccctgat ttccggaagc gagcgaacag tccacaaact
      181 gttgaacgtg gtgtcagcgg cggacaggga taagccctcg tcagcagttc cgtcagcact
      241 gcgacagcgt tctttacccg gtcaaaacag cgttgcggtg caccacgcct gacggatgaa
      301 ctgcgtgctc agggttacca ctttaacgta aaaaccgtgg cggcaagcct gcgccgtcag
      361 gggctgaggg caaaggcctc ccggaagttc agcacggtca gttaccgcgc acatggcctg
      421 cccgtgtcag aaaatctgct ggagcaggat ttttacgcca gtggtccgaa ccagaagtgg
      481 gcgggagaca tcacgtactt acgtacagat gaagttcggt tacatcctgt cagcacggaa
      541 cctcacgctt tttgagcggc acgacggatc ccccccaccc agtatccgac agacttttca
      601 ctgcgttcgt aaaaaccctt cttccctccc agcatatcca ctaattccgg tgacatatcc
      661 tgcagataag tctgcaaatc atgccccaga tcttccggtg actgcaactc ataaaccgcg
      721 cgccacacat caccctgcct tattttaagc cccgggttaa gaatatactg tccgtggttc
      781 agcctcagaa aaagcttttt tccataagga tttttgctgt taacttcatg ttgagagagc
      841 tggctgctga tataactcct tcgtttgcgg cgttccggca gcaacgcttc aggaagatga
      901 accacacact ctgccagtcg tgccgcactg aaggcttccg aaaaaaacag agcacttccc
      961 agatgcgtgt ggaacagcgc ctgcatcagg atcaccagaa acattgccat ctgtcggttg
     1021 tccagtttga tgaggcgtcc ttccacctgc agtgacagac tttcaggttt cagccgggta
     1081 tacaactgcg cacttttctg ttgcgcataa cggggcgtac tgaccgccgc tgacagaact
     1141 tgatgataag ccgccagccc gtcattaccg gtcagcaagg gatctgcacc acgctccagc
     1201 agtaactgaa ccagtgcaat attacctgcc cgggctgctg acatcagcgg agtaagatta
     1261 aacggcgaac ggtgatcaac accataacgt tcaatatccc gcaacacggc aaccgggtta
     1321 ttcattgagt atggacggaa atacttcagc tccagtaact gcagagtctt cgttctgggc
     1381 tgccttgtgg gcttaaaatc ttcccggtac agtgcattat acagcgggaa cagggaataa
     1441 atcagactgt actccagcgc ctgcagttgt atggttttat cggctgtacc tgcgaggata
     1501 tgctgtttcc actgtcgtgc atcctctgcg gtgataatct gccatggcat ctcagatgtc
     1561 tgcaggatcc ggctacggat ggtgtccgcc tgctcctgct tcccctgttt ttcaagcaga
     1621 tgtgcggttt tctgccactc atcacggctg gaaatctctt cctgcagatt cagaggctct
     1681 tcctgatgcg tgagcgcaag cagactccac agaggatgct ccacctgctg ttcaacaaga
     1741 tacacattgt gtgtagcacg ggtgagcgca acatacagcg cgttggtaaa aaatttatag
     1801 atttccgctg aacgatcctg cctgtcccgc ggacgggaat aacgtgcctc gccctcaaga
     1861 tcagccggtg taagaccctc gcaaatatca tcaaatgcct gccgggcagc gcttactata
     1921 ttgtaaagga tcaccgtttc gtattccagc cccttaacct cctgaaccga aaagaccagc
     1981 ggcgtactga accagcatcg cgcctgtgct ttctgttccg gatgcatcac aatgaccgct
     2041 acacgattcg aaagtgaggt tttggcattc agttcctgct ttgtctcttc cctgtcgtcc
     2101 agcaaccgga tggtcccttc tgcctgaccg cagcttctga caaaatggtg actttcccga
     2161 tcaatagcac taaagcggac ctgtttgagt ctcagcaacc ggttagccag tgcagtgacg
     2221 tggtgcccgt tacggtaatt cgcctgcagg atattaacgg ttgtgtcgtt tccctgttgc
     2281 tgccggaaaa agagactttt gagcgaactc catgagaaaa agctcggatg aactatctga
     2341 ttggcatcac cacagagtaa aaactgaccg ggatgacgta gcgttttcag taccagttgc
     2401 agttgaacag gcgtcatatc ctgaacttca tcaacaaaaa tgacatcata acggggagtt
     2461 gccaacgaaa gatactgatg actgagcaga ttactgtcat actgatgcga ctgtttcagc
     2521 catgcgatat aacgttcaaa caggacatag accgtcgggc ggtcctccat gccatagagc
     2581 gactgccgta tccccagtga cagatacgcc tcccgggaca gcggcccatt cccggaggca
     2641 accgcaccga tcacgcccct gaactcctca tacagggtat gggcagcccc cagtgcggcg
     2701 atagccctgt tgcggggcag ccagtcgctg aacgcactga aagtcacctc cctgccttcc
     2761 ggaatgcgca gagtttccag aaattccgtc agcgaaagaa agtcaatatt ctgtacctca
     2821 ctcccttccc ctgaagcgtc atagagagta cgcgcttttt ccaccagaaa tgaggaaagg
     2881 ctgagataca gtatatcacc agcagcctgc ttcatttttt ccagtaccag cgccgtctta
     2941 ccacttcccg ccgagccaac aatcaccagc ggtagcgggt gttcgtacag tgcctgctga
     3001 tcatcatcaa aggacagcat tttatccagg cggtggaaac gcccatgaga cggattgata
     3061 tacgtcagcg cttcagtcgc gatatcaaca ggatccggca ctggttgttg ttgtaagcgc
     3121 ccttcatcaa tcaccacgtt tctgcgaaga aaacgtgaag tgttataggc atgattgcga
     3181 atatattcca gaacaaggca gatagtttca ccgtggtagc gatacagcga aaacagtaat
     3241 cggtcccgga tactgagccg ggcgcggtaa agattggtgt caattttacg aacatcagcc
     3301 tgaacaaagt tatcggtggc aatagcctgt gcaaattttt tataacccgg gatcgtgtca
     3361 ggattcagag tatgataaag acagattttc atggctgcct gtttccgtcg tgcaaaaaac
     3421 atgctgattt tacgaaggaa tgccttactg aacaaccttg cccggaatac cccccgaaat
     3481 cagatggaac aatcagaaaa atgctctgca gttatggaat aaagaaggtg gggtggtacg
     3541 ccgggagggc gatccgctgg aaatggcgca gtagctgcag gcctgtcatg atgccggaat
     3601 agaagttcgt gtacaggtta atgaaagcgt caccccgtaa ataaactctg cgctgaaatt
     3661 cctttttttc attggagcac ctgtgttgtt ctgaggtgaa catatcacct ctgttcaggt
     3721 agccaaattc agtgtgccac ctcaaggttc ttagtgagtc gtttttaaaa gatatgataa
     3781 tatatcattg tttatgaatt gttgatgttt aatttagtaa attatatcaa atatgccatg
     3841 ggtttgctat gttattcatc cagtatctgg aaataaaatg caccagcttt attgggtttt
     3901 ccttttgtct ctgcttacta ggtgccagaa actttaaatg ttaaacgaag ggggcatttt
     3961 gttcaaagcc aatcatgttc cggtattaat gtatcatcat gtcagtcatt gccccggact
     4021 ggttacactt tctcccgtga cattccggaa gcaaatgaaa tggttggctg aaaacaactg
     4081 gaaaacactg tcatcagatg aactggagtt tttttatcgg ggcggaaaac tcccccggaa
     4141 aagcgttatg ttgacatttg atgatggata ccttgacaac tggtttcagg tatatccttt
     4201 attaaaagag tttaatctca aagcacatat ttttttaatt accggtttta ttgggaatgg
     4261 tccggttcgt cactctcctg gaaaggaata ttctcatcgc gattgcgaac atcaaatagc
     4321 gacagggaat gctgataatg ttatgcttcg ctggtctgaa gtaaacgaaa tgttgcaatc
     4381 tggtctggtc gaatttcatg ttcatacgca tactcataca cgctgggata aaaagttcag
     4441 ttcacgagaa gaacagtgta aacatcttcg gcaggacttg ttgagcggga gagaatattt
     4501 gaaagaaatg acaggaaaat gcagtaaaca tttgtgttgg ccggaaggtt attataacaa
     4561 ggattatatt caggtcgctg aagaacttgg attctattat ttatatacaa cagaaagaag
     4621 aatgaatgct cctgctgaag gaactacacg gataggaaga ataagcacca aagaacggga
     4681 aagttgcgca tggctgaaaa gacgactttt ttattataca acaccgtttt tttcatcgtt
     4741 gctggccttt cataaggggc caagattacc ggatgattaa taatgaatat actatttacg
     4801 gaatcatcac cgaatatagg tggtcaggaa ttacaggctg ttgctcaaat gaaggccctg
     4861 aagaaaatgg ggcattcagt tctgcttgtc tgcagggaaa acagcaaaat tgcttttgaa
     4921 gccagtaaat tgggaattga tatcacattc gcgttatttc gaaacagtct tcacatccct
     4981 actgcatgga gattactcgg aatagttcat ggttttcagc ccaatgcaat cgtttgtcac
     5041 agtgggcatg atagcaatat tgttggttta gtacggttat tcacttggaa acatccattc
     5101 agaattatca ggcaaaagac atatttgaca cgaaaaacaa aagttttttc aataaatcat
     5161 ttttgcgatg aggtgattgt tcccggaaca agtatgaaga cacatctgga gcaggaagga
     5221 tgtcgaaccc gggttactgt tgtgcctcca ggctttgatt tccagaaatt atacgttgat
     5281 tcccgaaaca gtttgcctcc aaatgttctt tcttggctgg cgtcccgaag gggatgcccc
     5341 gttattgctc aggtaggaat gttgcgcccg gaaaaagggc acgaatttat gttgaattta
     5401 ctgttccatt taaaaatgaa tggacgacag ttctgttggt tgattgtggg gtctggttcg
     5461 cctgaactgc gggagcattt acagtatcag attgacagta tgggcatgca tgatgatgtt
     5521 tttattgctg acaatgtttt tcctgccgcc cccgtatatc gggttgccag tctggtggtt
     5581 ctgccttcag aaaacgaatc gtttggtatg gtactggcag aagcatcggc attttctgtg
     5641 cctgtactgg ccagtcagat tggtggaatc cctgatgtta ttcagaacaa ccagaccggg
     5701 acattgttac cagcaggtaa taagcacgca tggatgtgcg ccctgaatga tttttttaat
     5761 gaccctgggc gtttttatca gatggctcgc caggcaaaac aggatataga agagcggttt
     5821 gatattaata aaactgcgtt aaaaatactc acattagcga agcacaagta acaatatgtt
     5881 ttctgtaagt aacttatcat ttatcggttt ccttaaaagg attgtttttt cctcagattc
     5941 actcccgggg aagtgggaac acagaaaatt ccggttcatg tacattttgc gatgtgctat
     6001 aaatccggtt gccagtattc gatattatta cgaactgcgt tccttgcagt gcattgagga
     6061 tattctggca atacaaccca cgttgccagc aagaattcat cgaccttatt tgcataaagg
     6121 ggggcg
//
LOCUS       DADLSA010000193         6072 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.194, whole genome shotgun sequence.
ACCESSION   DADLSA010000193 DADLSA010000000
VERSION     DADLSA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.194"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..569
                     /gene="yneK"
                     /locus_tag="JN691_003769"
     CDS             <1..569
                     /gene="yneK"
                     /locus_tag="JN691_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY7245066.1"
                     /translation="HHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKD
                     PNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQESPHFQDLFL
                     FFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHE
                     DLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            719..1909
                     /gene="ydeA"
                     /locus_tag="JN691_003770"
     CDS             719..1909
                     /gene="ydeA"
                     /locus_tag="JN691_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAY7245067.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQAQ"
     gene            complement(1934..2599)
                     /gene="marC"
                     /locus_tag="JN691_003771"
     CDS             complement(1934..2599)
                     /gene="marC"
                     /locus_tag="JN691_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAY7245068.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            2811..3245
                     /gene="marR"
                     /locus_tag="JN691_003772"
     CDS             2811..3245
                     /gene="marR"
                     /locus_tag="JN691_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAY7245069.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3265..3648
                     /gene="marA"
                     /locus_tag="JN691_003773"
     CDS             3265..3648
                     /gene="marA"
                     /locus_tag="JN691_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HAY7245070.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3680..3898
                     /gene="marB"
                     /locus_tag="JN691_003774"
     CDS             3680..3898
                     /gene="marB"
                     /locus_tag="JN691_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAY7245071.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(3929..4756)
                     /locus_tag="JN691_003775"
     CDS             complement(3929..4756)
                     /locus_tag="JN691_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HAY7245072.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATLVPVVGLASAALLLDERLTGLQFLGAVLIMTGLYINVFGLRWRKAVKVGS"
     gene            4951..>6072
                     /gene="ydeE"
                     /locus_tag="JN691_003776"
     CDS             4951..>6072
                     /gene="ydeE"
                     /locus_tag="JN691_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HAY7245073.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNNLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVII"
BASE COUNT         1545 a         1530 c         1396 g         1601 t
ORIGIN      
        1 atcaccatac cgctgcagat tacttacgcc agtttgattt gcttgtcctg aattttatct
       61 ctcgcggtaa ttttgtcata ctcccccgat tatggaataa ctctgaggtt cacagatggt
      121 tcgtcaataa agatcctaac ttgatcaccg ccattctcga cataacggac agcgaattaa
      181 aagaggactt attgcaaagc ctgatggatt cattaggttc taacaaacat gtacaacccg
      241 aagtctgcat ctgcttctta tcccttttag cagaacaaga atctcctcat tttcaggact
      301 tgtttttatt tttcgccaat atgttattgc actatcacca atttatgaat cccaatgaaa
      361 gtgatctgaa tgacgtgttg atgccagcat cattaagtga tgataaaatt atcaaacata
      421 tggcgcgcag gaccctcaaa ctgtttgtaa aaaatgaaac gccgccaaaa gttactcacg
      481 aagacctggt gaaaaacagg cctcgctccc ctgtcagacc acctataccc gcaaccgcca
      541 aaacgccaga cctccctgaa cgtcattaaa ccgtgatgtt accgactctc tgacgcggaa
      601 cagaatcagc gtcagagaaa cggaaaacgc gatccagatc acaaatgcat tgtattcaca
      661 tcattaaccg ttttaagatc atttcatcac tttttcgcaa ctcacccgat aatctgttat
      721 gacaacaaac actgtttccc gcaaagtggc gtggctacgg gtcgttacgc tggcagtcgc
      781 cgccttcatc ttcaacacca ccgaatttgt ccctgttggc ctgctctctg acattgcgca
      841 aagttttcac atgcaaaccg ctcaggtcgg catcatgttg accatttacg catgggtagt
      901 agcgctaatg tcattgcctt ttatgttaat gaccagtcag gttgaacggc gcaaattact
      961 gatctgcctg tttgtggtgt ttattgccag ccacgtactg tcgtttttat cgtggagctt
     1021 taccgttctg gtgatcagtc gcattggtgt ggcttttgca catgcgattt tctggtcgat
     1081 tacggcgtct ctggcgatcc gtatggctcc ggccgggaag cgggcacagg ccttgagttt
     1141 aattgccacc ggtacagcac tggcgatggt cttaggttta ccgctcgggc gcattgtggg
     1201 gcagtatttc ggctggcgaa tgaccttctt cgcgattggt attggggcgc ttgttaccct
     1261 tttgtgcctg attaagttac ttcccttact gcctagtgag cattccggtt cattgaaaag
     1321 cctcccgcta ttgttccgcc gcccggcatt gatgagcatt tatttgttaa ctgtggtggt
     1381 tgtcaccgcc cattacacgg catacagcta tatcgagcct tttgtgcaaa acattgcggg
     1441 attcagcgcc aactttgcca cggcattact gttattactc ggtggtgcgg gcattattgg
     1501 cagcgtgatt ttcggtaaac tgggtaatca atatgcgtct gcgttggtaa gtacggcgat
     1561 tgcgctgttg ctggtgtgtc tggcactgct gctacctgcg gcgaacagtg aaatacacct
     1621 cggggtgctg agtattttct gggggatcgc gatgatgatc atcgggcttg gtatgcaggt
     1681 taaagtgctg gcgctggcac cagatgctac cgacgtcgcg atggcgctat tctccggcat
     1741 atttaatatt ggaatcgggg cgggtgcgtt ggtaggtaat caggtgagtt tgcactggtc
     1801 aatgtcgatg attggttatg tgggcgcggt gcctgctttt gccgcgttaa tatggtcaat
     1861 cattatattt cgccgctggc cagtgacact cgaagaacag gcgcaatagt tgaaaggccc
     1921 attcgggcct tttttaatgg tacgttttaa tgatttccaa gatgccgtta ataataaact
     1981 gcacacccat acataccagc aggaacccca tcaggcggga gatcgcttca atgccaccct
     2041 tgcccaccag ccgcataatt gcgccggagc tgcgtaggct tccccacaaa ataaccgcca
     2101 ccaggaaaaa gatcagcggc ggcgcaacca tcagtaccca atcagcgaag gttgaactct
     2161 gacgcactgt ggacgccgag ctaataatca tcgctatggt tcccggaccg gcggtacttg
     2221 gcattgccag tggtacaaag gcaatattgg cactgggttc atcttccagc tcttccgact
     2281 tgcttttcgc ctccggtgaa tcaatcgctt tctgttgcgg aaaaagcatc cgaaaaccga
     2341 taaacgcgac gattaagccg cctgcaattc gcagaccggg aatcgaaatg ccaaatgtat
     2401 ccatcaccag ttgcccagcg taatacgcca ccatcatgat ggcaaatacg tacaccgagg
     2461 ccatcaacga ctgacgatta cgttcagcac tgttcatgtt gcctgccagg ccaagaaata
     2521 gcgcgacagt tgttaatggg ttagctaacg gcagcaacac caccagcccc aggccaattg
     2581 ctttaaacaa atctaacatt ggtggttgtt atcctgtgta tctgggttat cagcgaaaag
     2641 tataaggggt aaacaaggat aaagtgtcac tctttagcta accttgcatc gcattgacca
     2701 aaacttgaac cgatttagca aaacgtggca tcggtcaatt cattcatttg acttatactt
     2761 gcctgggcaa tattatcccc tgcaactaat tacttgccag ggcaactaat gtgaaaagta
     2821 ccagcgatct gttcaatgaa attattccat tgggtcgctt aatccatatg gttaatcaga
     2881 agaaagatcg tctgcttaac gagtatctgt ctccgctgga tattaccgcg gcacagttta
     2941 aggtgctctg ctctatccgc tgcgcggcgt gtattactcc ggttgaactg aaaaaagtgt
     3001 tgtcggtcga cctgggagca ctgacccgta tgctggatcg cctggtctgt aaaggctggg
     3061 ttgaaaggtt gccgaacccg aatgataagc gcggcgtact ggtaaaactt accaccagcg
     3121 gcgcggcaat atgcgaacaa tgccatcaat tagttggcca ggacctgcat caagaattaa
     3181 caaaaaacct gacggcggac gaagttgcaa cacttgagca tttgcttaag aaagtcctgc
     3241 cgtaaacaaa aaagaggtat gacgatgtcc agacgcaata ctgacgctat taccattcat
     3301 agcattttgg actggatcga ggacaacctg gaatcgccac tgtcactgga gaaagtgtca
     3361 gagcgttcgg gttactccaa atggcacctg caacggatgt ttaaaaaaga aaccggtcat
     3421 tcattaggcc aatacatccg cagccgtaag atgacggaaa tcgcgcaaaa gctgaaggaa
     3481 agtaacgagc cgatactcta tctggcagaa cgatatggct tcgagtcgca acaaactctg
     3541 acccgaacct tcaaaaatta ctttgatgtt ccgccgcata aataccggat gaccaatatg
     3601 cagggcgaat cgcgcttttt acatccatta aatcattaca acagctagtt gataacgtga
     3661 caacgtcact gaggcaatca tgaaaccact ttcatccgca atagcagctg cgcttattct
     3721 cttttccgcg cagggcgttg cggaacaaac cacgcagcca gttgttactt cttgtgccaa
     3781 tgtcgtggtt gttcccccat cgcaggaaca accaccgttt gatttaaatc acatgggtac
     3841 tggcagtgat aagtcggatg cgctcggcgt gccctattat aatcaacacg ctatgtagtt
     3901 tgttctggcc ccgacatctc ggggcttatt aacttcccac ctttaccgct ttacgccacc
     3961 gcaagccaaa tacattgata tacagcccgg tcataatgag caccgcacct aaaaattgca
     4021 gacccgttaa gcgttcatcc aacaatagtg ccgcacttgc cagtccgact acgggcacca
     4081 gtgtcgccac aaacgccaga tacatcagcg acaagatggt ggtcatatca atagtaacca
     4141 gactgtgaat catggatgcg gaaccatcga gaatcagcga ggcaacaaag aagggaatga
     4201 ttgggattaa agcgctccag attaccagcg acatcaccgc cggacgcgtt gagtgcgaca
     4261 tgatcttttt attgaagatg ttgccacacg cccaactaaa tgctgcagcc agggtcaaca
     4321 taaagccgag catcgccaca tgctgaccgt tcagactatc ttcgattaac accagtacgc
     4381 caaaaatcgc taaggcgatc cccgccaatt gtttgccatg cagtcgctcc ccgaaagtaa
     4441 acgcgccaag cacgatagta aaaaacgcct gtgcctgtaa caccagcgaa gccagtccag
     4501 caggcatacc aaagttaatg gcgcagaaca gaaaggcaaa ctgcgcaaaa ctgatggtta
     4561 atccataccc caagagcaaa ttcagtggta ctttcggtcg tgcaacaaaa aagatggccg
     4621 gaaaagcgac cagcataaag cgcaaaccgg ccagcatcag cggtggcatg ttatgaagcc
     4681 ccactttgat gaccacaaaa tttagccccc atacgaccac taccagtagc gccaacaccc
     4741 catcttttcg cgacattcta ccgcctctga atttcatctt ttgtaagcaa tcaacttagc
     4801 tgaatttact tttctttaac agttgattcg ttagtcgccg gttacgacgg cattaatgcg
     4861 caaataagtc gctatacttc ggatttttgc catgctattt ctttacatct ctaaaataaa
     4921 acataacgaa acgcactgcc ggacagacaa atgaacttat ccctacgacg ctctaccagc
     4981 gcccttcttg cctcgtcgtt gttattaacc atcggacgcg gcgctacgct gccatttatg
     5041 accatttact tgagtcgcca gtacagcctg agtgtcgatc taatcggtta tgcgatgaca
     5101 attgcgctca ctattggcgt cgtttttagc ctcggttttg gtatcctggc ggataagttc
     5161 gacaagaaac gctatatgtt actggcaatt accgccttcg ccagcggttt tattgccatt
     5221 cctttagtga ataacgtgac gctggttgtg ctcttttttg ccctcattaa ctgcgcctat
     5281 tctgtttttg ctaccgtgct gaaagcctgg tttgccgaca atctttcgtc caccagcaaa
     5341 acgaaaatct tctcaatcaa ctacaccatg ctaaacattg gctggaccat cggtccgccg
     5401 ctcggcacgc tgttggtgat gcagagcatc aatctgccct tttggctggc agctatctgt
     5461 tccgcgtttc ccatgctttt cattcaaata tgggtaaagc gcagcgagaa aatcatcgcc
     5521 acggaaacag gcagtgtctg gtcgccgaaa gttttattac aagataaagc actgttgtgg
     5581 tttacctgct ctggttttct ggcttctttt gtaagcggcg catttgcttc atgcatttca
     5641 caatatgtga tggtgattgc tgatggggat tttgccgaaa aggtggtcgc ggttgttctt
     5701 ccggtgaatg ctgccatggt ggttacgttg caatattccg tgggccgccg acttaaccca
     5761 gctaacatcc gcgcgctgat gacagcaggc accctctgtt tcgtcatcgg actggtcggt
     5821 tttatttttt ccggcaacaa cctgctattg tggggtatgt cagctgcggt atttactgtc
     5881 ggtgaaatca tttatgcgcc gggcgagtat atgttgattg accatattgc gccgccagga
     5941 atgaaagcca gctatttttc cgcccagtct ttaggctggc ttggtgccgc gattaaccca
     6001 ttagtgagtg gcgtagtgct aaccagcctg ccgccttcct cgctgtttgt catcttagcg
     6061 ttggtgatca tt
//
LOCUS       DADLSA010000194         5960 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.195, whole genome shotgun sequence.
ACCESSION   DADLSA010000194 DADLSA010000000
VERSION     DADLSA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.195"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..149
                     /locus_tag="JN691_003777"
     CDS             <1..149
                     /locus_tag="JN691_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HAY7245074.1"
                     /translation="PDNEGHVSVRYAAANNLSVIGATKEDKQRISEILQETWESADDW
                     FVSE"
     gene            439..693
                     /gene="bssS"
                     /locus_tag="JN691_003778"
     CDS             439..693
                     /gene="bssS"
                     /locus_tag="JN691_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAY7245075.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            808..1926
                     /gene="solA"
                     /locus_tag="JN691_003779"
     CDS             808..1926
                     /gene="solA"
                     /locus_tag="JN691_003779"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAY7245076.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            1974..2087
                     /locus_tag="JN691_003780"
     CDS             1974..2087
                     /locus_tag="JN691_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAY7245077.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2348..2914
                     /locus_tag="JN691_003781"
     CDS             2348..2914
                     /locus_tag="JN691_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAY7245078.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLLRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2918..3493
                     /locus_tag="JN691_003782"
     CDS             2918..3493
                     /locus_tag="JN691_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAY7245079.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3535..4587)
                     /locus_tag="JN691_003783"
     CDS             complement(3535..4587)
                     /locus_tag="JN691_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAY7245080.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4812..5732
                     /locus_tag="JN691_003784"
     CDS             4812..5732
                     /locus_tag="JN691_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAY7245081.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RITRWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
BASE COUNT         1528 a         1451 c         1404 g         1577 t
ORIGIN      
        1 ttcctgataa tgaaggccac gtatcggtac gttatgccgc agcgaataat ttatcggtta
       61 ttggcgcaac aaaagaagat aaacagcgca ttagcgaaat tctccaggaa acgtgggaaa
      121 gcgccgatga ctggtttgtc agcgaataat atgcagtgat ttttgttttt gccgggtcgc
      181 cccggctttt ttattttact tacgaatttt gagtacaaaa tttctacact tcctcaaaat
      241 aattgcacaa tatgctaaaa aaatatgaat gacatgctgt tttattgttc atataaatct
      301 taaaagcaga aatttatctg ttcccccctt taaaaattcg gtgaataccc ttacttattg
      361 gtatactgaa gttgcttcag ataaaaacgc attgaacctc gaataacgtt gtctagtaac
      421 acgaattagg gggccatgat ggaaaaaaat aatgaagtca ttcagactca tccgctcgta
      481 gggtgggaca tcagcaccgt tgatagctat gatgcgctga tgttgcgttt gcactaccag
      541 accccaaata agtccgagca ggaagggact gaagttggtc agacgctctg gttaaccact
      601 gatgttgcca gacaatttat ttcgatatta gaagcaggaa tcgccaaaat tgaatccggt
      661 gatttcccgg taaacgagta tcggcgtcat taacatgaca tgaccatcca cccaaaaggc
      721 acctcaccgg tgcctttacc atttctgggt tgtatatctg cattcagtta attactctgc
      781 ttaaaccatg agagcagaga gaaaaaaatg aaatacgatc tcatcattat tggcagcggt
      841 tccgtaggcg ctgccgccgg gtattatgca acccgcgccg gtttaaacgt gctaatgacc
      901 gacgcccata tgccaccgca tcaacacggc agccaccacg gcgatacgcg attaattcgc
      961 catgcttatg gtgaaggcga aaagtatgtc ccgttggtcc tccgcgcgca aacgctgtgg
     1021 gatgaacttt cccgccacaa cgaagatgat cccatttttg tacgctctgg tgtcattaac
     1081 ctcggcccgg ctgactccgc atttctcgcc aacgtcgccc acagcgccga acagtggcaa
     1141 ctcaacgttg aaaagctcga tgcgcaaggg attatggccc gctggccaga aatacgcgtc
     1201 ccggacaact acatcggctt atttgagact gattccggtt ttttgcgcag cgaactggcg
     1261 attaaaacct ggatccaact ggcgaaggaa gcgggctgtg cgcaactgtt caactgcccg
     1321 gtcaccgcaa ttcgtcatga cgatgatggc gtaactattg aaacggttga cggtgagtat
     1381 caggcgaaaa aagcgattgt ctgcgcggga acatgggtaa aagacctgct cccggagctg
     1441 cctgtccagc ccgtacgtaa agtatttgcc tggtatcagg ccgatggccg ctatagcgtg
     1501 aagaataaat tcccggcgtt taccggtgaa ctgcccaatg gcgatcaata ttatggtttt
     1561 ccggcagaaa acgacgcgtt gaagattggc aaacataacg gaggccaggt tatccattca
     1621 gcggatgaac gtgttccgtt tgcggaagtg gccagtgatg ggtcagaagc cttcccgttc
     1681 ttgcgcaatg tattgccggg tatcggttgc tgcctgtacg gcgctgcctg cacctatgat
     1741 aattcgcctg acgaagattt tattatcgat accctacccg gccacgataa tacactgctc
     1801 attaccggcc tgagtgggca cggttttaaa tttgcgtcag ttttagggga aatagctgcc
     1861 gattttgcgc aagaccaaaa aagcgatttt gatttgacgc cattcaggct ttcccgcttc
     1921 caataatcat cattacggcc tccagaatga ggccgttttt acaggagtat ttaatgcgtc
     1981 ggctgttgca ctatctcatc aataatattc gcgagcatct gatgctttat ctttttctct
     2041 ggggattgtt ggcaattatg gatctgatct atgtgtttta tttttagttt tccgcactga
     2101 cataaactga caaatattta atctcccttt catttagcgt gaaaaattga atgagattat
     2161 tcctgcttct acagcactca ccagacatct ctgatgctga acccactaaa cttatgaaga
     2221 ttctttcatt attaattcaa aattattgaa ataaaaataa atatcattca atttgttatt
     2281 tttgtgttgc aattcattct ccgacacgcc attttctttc tctcgcaaat tcgtattgga
     2341 tgcacttatg tcattcacaa atacccctga acgctatggt gttatatctg cggccttcca
     2401 ttggctaagc gcaattattg tctacggcat gtttgcttta ggtctgtgga tggtcacgct
     2461 cagttattac gatggctggt atcacaaagc acccgaactg cataaaagca tcggtatttt
     2521 gttaatgatg gggctggtta ttcgcgttct gttgcgtgtc atatctccac cgccgggtcc
     2581 gctgccgagt tattcgccca tgactcgtct tgccgcaaaa gctggacatc tcgcattgta
     2641 cctattgctg ttcgccatcg gcatcagcgg ctatctgatc tcaactgccg atggtaaacc
     2701 gatcagcgtt tttggctggt ttgacgtccc cgcgaccctt gccgacgctg gcgcacaggc
     2761 agactttgcc ggtgccctgc atttttggct cgcgtggagc gtcgtagtac tgtctgttat
     2821 gcacggattt atggccctga agcatcattt catcgataaa gacgacactc tgaagcgcat
     2881 gctgggaaag tcgtcatctg actatggagt ataaaaaatg aaaaaaagcc tgcttggttt
     2941 aaccttcgcg tccctgatgt tctctgctgg ttcagcggtt gccgccgatt acaaaattga
     3001 caaagaaggt cagcacgcct ttgttaactt ccgcatacag catcttggct atagctggtt
     3061 atatggcacc tttaaagatt tcgacggtac ttttaccttt gatgaaaaaa atccggctgc
     3121 cgataaagtg aatgtgacaa ttaacaccac cagcgtcgat actaaccacg ccgaacgcga
     3181 taaacatctt cgcagtgcgg attttctcaa taccacaaaa tatccacagg caacattcac
     3241 ctccaccagc gtgaagaaag acggtgacga actggatatt accggcgatc tgacgctgaa
     3301 tggcgtaacc aaacccgtca cgctggaagc gaaattaatt ggtcagggcg acgacccatg
     3361 gggcggtaaa cgtgcaggct ttgaggccga aggcaaaatt aagctcaaag acttcaatat
     3421 caagacagat ttaggtccag cttctcagga agtggatctg attatttcag tggaaggcgt
     3481 acagcagaag taagagtgaa atgttgccgg atggcacgca tccggcaatg atatttattc
     3541 tgttggatca ggaataccca gtgttgtatt cagacgtcca cgagacttat taaagatctt
     3601 attgccattt tcacgtcctg cccgacggcg tcgctgttct tctggcggta acgcgctttc
     3661 ttcgcagcaa atctcactac aacaaccttt gtatttttcc gcgcatactg gacactgaat
     3721 aaacagcagg tggcagccat catttttaca gttggtatgg ctgtcgcacg gcgcgccgca
     3781 ctggtggcaa tgcgcgataa tctcatccga tatacgttcg cccatccgct cgtcaaaaac
     3841 aaaatttttg ccaataaaac gcaccggcaa gccctgctcg cgcgccttac gggcgtattc
     3901 aataattccg ccctcgatat gccagacttt gttgaatccg ttatgtttca tccaggcact
     3961 ggccttttca caacgaatgc cgccggtgca gtacatgacg atttttttat ctttatgtgc
     4021 ctgcatcatc tcgactgctt ttggcagctg ctcacggaag gtatctgccg gaatttcgag
     4081 cgcgttttca aagtgcccca cttcatactc atagtggtta cgcatgtcga taaatagtgc
     4141 atcgggatcg tcaagcatgg cgttcacttc cgccgcttgc agatactcac caacattgct
     4201 ggcatcaaag tgaggatcgt caataccgtc ggcaacaatg cgatcgcgta ccttcatgcg
     4261 cagtacccag aaggatttcc cgtcatcatc caacgcgata ttcaggcgta aaccctctaa
     4321 agccgggtcg aaggcataga gctgcgcgcg aaatgtttca acattgctcg caggtacgct
     4381 gatttgcgcg ttaatgccct catgcgccag atacactcgc ccaaaaacat tcagcgcggt
     4441 aaacagctga tataaagcgt cacgggtcgc cttaggatcg gcgatgtgga aatacttgta
     4501 aaacgaaatg gtggttcgcg gttcgctctc agccaacatt ttggctttta acgcgtcgtt
     4561 ggaaatgcgg ttgtgtaaca ctggcatggt gtacggttcc tgcgagatgg gaaagtaaaa
     4621 atccgcggca tgatatagca attatcgata attaacatcc acacatttta cgctacattt
     4681 gcgcattaaa aattatttgt tatttacaag cgcggcaatt tcgcccagtc ttcagccaca
     4741 aattttggtt gcgggcgaaa aaatgcgaca atacatacaa ttgcccgtat aggttgaaaa
     4801 acaggattga tatgacgaat ctacccaagt tctccaccgc actgcttcat ccgcgttatt
     4861 ggttaacctg gttgggtatt ggcgtacttt ggttggtcgt gcaattgccc tacccgatta
     4921 tctaccgcct cggttgtgga ttaggaaaac tggcgttacg ttttatgaaa cgacgcgcaa
     4981 aaattgtgca tcgcaacctg gaactgtgct tcccggaaat gagcgaacaa gaacgccgta
     5041 aaatggtggt gaagaatttc gaatccgttg gcatgggcct gatggaaacc ggtatggcgt
     5101 ggttctggcc ggaccgccga atcacccgct ggacggaagt gatcggcatg gaacacattc
     5161 gtgacgtgca ggcgcaaaaa cgcggcatcc tgttagttgg catccatttt ctgacactgg
     5221 agctgggcgc gcggcagttt ggtatgcagg aaccggggat tggcgtttac cgcccgaacg
     5281 ataatccact gattgactgg ctacaaacct ggggccgttt gcgctcaaat aaatcgatgc
     5341 tcgaccgcaa agatttaaaa ggcatgatta aagccctgaa aaaaggcgaa gtggtctggt
     5401 acgcaccgga tcatgattac ggcccgcgct caagcgtttt cgtcccgtta tttgccgttg
     5461 agcaggctgc gaccacgacc ggaacctgga tgctggcacg gatgtccggc gcatgtctgg
     5521 tgcccttcgt tccacgccgt aagccagatg gcaaagggta tcaattgatt atgctgccgc
     5581 cagagtgttc tccgccactg gatgatgccg aaactaccgc cgcgtggatg aacaaagtgg
     5641 tcgaaaaatg catcatgatg gcaccagagc agtatatgtg gttacaccgt cgctttaaaa
     5701 cacgcccgga aggcgttcct tcacgctatt aaatctccca tgccggatgc ttcagaatgg
     5761 catccggcat taccacagca aatccccctg atttagcgat aaaagctctc tggattgcgc
     5821 cccctggaag tcgggcgcat aattagtgtg tttatctttt cttcttatgt tcaccgcgcc
     5881 tggcgcacca acagcggatt gctatgtcac cctgtgaaaa tgacacccct ataaactgga
     5941 aacgaaacct gatcgtcgcc
//
LOCUS       DADLSA010000195         5898 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.196, whole genome shotgun sequence.
ACCESSION   DADLSA010000195 DADLSA010000000
VERSION     DADLSA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.196"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..886)
                     /gene="mltC"
                     /locus_tag="JN691_003785"
     CDS             complement(<1..886)
                     /gene="mltC"
                     /locus_tag="JN691_003785"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY7245082.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAV"
     gene            complement(951..1226)
                     /locus_tag="JN691_003786"
     CDS             complement(951..1226)
                     /locus_tag="JN691_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAY7245083.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(1254..2306)
                     /gene="mutY"
                     /locus_tag="JN691_003787"
     CDS             complement(1254..2306)
                     /gene="mutY"
                     /locus_tag="JN691_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY7245084.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTSCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            2467..3186
                     /gene="trmB"
                     /locus_tag="JN691_003788"
     CDS             2467..3186
                     /gene="trmB"
                     /locus_tag="JN691_003788"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAY7245085.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3186..3512
                     /locus_tag="JN691_003789"
     CDS             3186..3512
                     /locus_tag="JN691_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAY7245086.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGRICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            3696..4415
                     /locus_tag="JN691_003790"
     CDS             3696..4415
                     /locus_tag="JN691_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAY7245087.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            4591..5637
                     /gene="ansB"
                     /locus_tag="JN691_003791"
     CDS             4591..5637
                     /gene="ansB"
                     /locus_tag="JN691_003791"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAY7245088.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            5754..>5898
                     /locus_tag="JN691_003792"
     CDS             5754..>5898
                     /locus_tag="JN691_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAY7245089.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAI"
BASE COUNT         1466 a         1475 c         1561 g         1396 t
ORIGIN      
        1 tgacggcata acgccgcgat gttgggttat caattccgcc gagataaaca ttgttcagca
       61 tcgccagata cgcggtgccg gtatcaatat tgctggcagg atcaaacaag aaactgcggc
      121 tcggcgtgcc ggatttcccc tgcgagcgga acacatcttt cccggcagta ttttgtacca
      181 cctgcattaa tcccagcgca tcggaacggc tgaccgcata cgggttaaag gaagattcag
      241 tctgcataat tgccagaatc agcgactcat caacgccata tttccgtgac gcctggcgga
      301 ccatgccgag atatttgtgc gcacgtttat caaggtggtt cggcaccatg ttaatggtga
      361 cgctgtagat gatacgtagc ccgttgctgc ggcttttcag acggtttttc agcagataat
      421 ccgcgaagtt gcttgcgcga ccttcccagc gaatcggctg cccggtgttg tccaccacct
      481 gaccgtaaag gaaaggttct ttcgaaattg taatatcatc aacgtcggaa tagagatcga
      541 ccgaactcgg atcgtcaccc atcagcaacg ttttgataat tgcccggcgc aaatgcgcgg
      601 caggttctgt cccggcgatg gtttcgatag taatcgtacc gtcatcgaag ttgatgtggc
      661 tgcgggtctg atattgatcg gtgtatttca cgtagtcctt aggaccggcg atcaccacct
      721 ctttgaagcc ccagatgttc tcaatattgt gggcaaattg ccccatcaga atatcaaaac
      781 cgttggtatc tttgacccag gcttcgttat aggtatcgcc ttttttggtc gtcgaacagg
      841 agatgagcaa cggcgcaatc aaagccagcg cgagatattt tttcatcatt ccgggtgcgt
      901 gttgtgtttg tttttatgca agctgccgga ggcgtgctcc ggcactgttt ttatttttta
      961 tcttccggcg tatagccctc gatatgcacc tctttaccct cgaacaggaa gttgaccatc
     1021 tcctgctcca gcagcttgcg gtgctcggca ttcatcatgt tgagtttctt ttcattaatc
     1081 agcatggttt gtttgtgctg ccactgcgcc caggcttctt tggagatctc gttatagatg
     1141 cgttttccca gctcgccggg gtacagctga aaatcctgac cttctgcttc acgttgcagg
     1201 aaagtacaaa aaatcgttct gctcataaat catcctcttt atcgactcac gcgctaaacc
     1261 ggcgcgccag tgcgtaactg ctgtaacaaa cgctccacgg gagccgccag gccaactgac
     1321 ggcggttgcg ctaagttata ccagagcgca ttgccttcat ccatgcagct ggtgaatgac
     1381 gacacgggaa gccacatagg cacaatatct aagtggaaat ggctgaaggt atggcgaaac
     1441 gcggtcagtt gcgtcaggtt atcggcagca atctgccgtt gtgccagcca atgccgcaaa
     1501 ctttcttcgt cggcaaactg cgggaaacag tataaaccgc cccacaatcc gctcggcgga
     1561 cgctgcgcca gcaatacttc atcttcgtgc tgtaacagga gaaagtagcc ggtgcgctcc
     1621 ggcagcgtct gtttcggttt tttgcccgga taaagcgacc agctattgtt ggtggcggca
     1681 atacatccgt tttgtagcgg acagagcgaa catttcggct tcgaacgcgt gcaaatcatc
     1741 gcgcccaaat ccatcatcgc ctgattaaac cgttccacgc cgaccgcggg cgtcacctgc
     1801 tcgctcaaac tccatagttt attctcgacc tcttttttcc caggccagcc gcttacagca
     1861 tagcagcgcg ccagcacgcg tttgacgtta ccgtcgagaa tcggaaagtg cttacccaga
     1921 gaaagcgaga gaatcgcgcc tgcggtggaa cgcccgacgc ccggtaacgc cgcgacttct
     1981 tcaaaggttt ccgggaattt accgccgtgt aaggtcgcca cttgttgtgc cgctttatgc
     2041 aggttgcgcg cgcgggcgta atagccaagc ccggtccaca agtggagaac ttcgtcgagc
     2101 ggcgcattgg cgagatcggt caccgtcggg aagtgcgcca taaagcgttc aaaatagggg
     2161 ataacggtcg caacctgagt ttgttgcaac atcacttctg agagccatac tttgtagggc
     2221 gtcttgtcaa tttgccaggg cagcgttttc cgcccgtatt tatcgtacca gtccagaacc
     2281 tgggctgaaa attgcgacgc ttgcatggtc accgacttca ctgttgttgg gggcaagatt
     2341 gcagcacagc gacggcaggg tgtaaaccgg aactttccgc agccacggcc ttatcatgct
     2401 tgcatccggc aactaacttt ggataatgcc cgttttcaga acactttcac aagcgactaa
     2461 atctttatga aaaacgacgt catttcaccg gaatttgatg aaaacggccg cccactgcgc
     2521 cgtatccgta gttttgtgcg ccgccagggg cgactgacca aaggccagga acatgcgctg
     2581 gaaaactact ggccggtgat gggcgttgag ttcagtgaag atatgctgga tttccccgcg
     2641 ctttttggcc gtgaagcgcc ggtgacgctt gagattggtt ttggcatggg ggcgtcgctg
     2701 gtggcaatgg ctaaagatcg ccccgagcag gacttcctcg gcattgaagt gcattcaccg
     2761 ggcgttggtg cgtgcctggc ttctgcgcat gaagaaggtt taagcaacct gcgcgtgatg
     2821 tgtcacgatg cggttgaagt gctgcataaa atgattcctg acaattcatt gcgcatggtg
     2881 cagctctttt tccctgaccc gtggcacaaa gcgcgccata ataaacgccg tatcgttcag
     2941 gtgccgtttg ccgaactggt aaaaagcaaa ctgcagctgg ggggcgtatt ccatatggcg
     3001 accgactggg aaccttatgc ggaacatatg cttgaagtga tgtcttctat tgacggttat
     3061 aaaaacctgt cagagagcaa tgattacgta ccgcgtccgg catcacgtcc ggtgacgaaa
     3121 tttgaacaac gtggtcatcg tcttggtcac ggagtatggg acttaatgtt cgagagggtg
     3181 aaataatggc aaagaaccgt agccgtcgtc tgcgtaaaaa aatgcacatc gacgaattcc
     3241 aggaattagg attttcggtg gcatggcgat tcccggaagg tacatcggaa gaacagattg
     3301 ataaaatcgt tgatgacttt attaacgagg ttatcgaacc gaacaaactg gcctttgacg
     3361 gcagcggtta tctggcctgg gaaggtcgga tctgcatgca ggaaatcggc aaatgcaccg
     3421 aagaacatca ggcgattgtg cgtaagtggc tggaagagtg caaactggat gaggtacgca
     3481 ccagcgaact tttcgacgtt tggtgggact aagaaagcat acgggcgatg acaaatgcaa
     3541 aactgcctga tgcgctacgc ttatcaggcc tacagagatg cacgatcgag taggcgggat
     3601 aaggcgttta cgccgcatcc ggcatggaaa acgcgtactt tgttatcaat ctggggccag
     3661 catatgctgg cctgatttgt tcttgaggga agactatgat gcgcaaaatg ctgctggcgg
     3721 cagcactttc agtgacggca atgaccgctc acgccgacta ccagtgcagc gtcacgccgc
     3781 gtgacgatgt gattgtcagc ccgcaaaccg tgcaggtgaa gggcgaaaac ggcaatctgg
     3841 tgatcacgcc agacggcaac gtgatgtata acggtaagca atattccctg aatgccgccc
     3901 agcgcgagca ggcgaaggat tatcaggctg aactacgtag cacgctgccg tggattgatg
     3961 aaggcgcgaa aagccgcgtc gagaaagccc gtattgcgct ggataaaatt atcgttcagg
     4021 agatgggcga cagcagcaag atgcgcagcc gtctgaccaa acttgatgcg cagcttaaag
     4081 agcagatgaa tcgcattatc gaaacgcgca gcgatggcct gacgttccac tataaagcca
     4141 ttgatcaggt tcgcgccgaa ggccagcaac tagtgaatca ggcaatgggc ggaattttac
     4201 aggacagcat taatgaaatg ggcgcgaaag cggtgctgaa aagcggcggt aacccattac
     4261 agaatgtgct gggaagcttg ggcggtctgc aatcctcaat ccaaaccgag tggaaaaagc
     4321 aggaaaaaga tttccagcag tttggcaaag atgtttgtag ccgcgttgtg actctggaag
     4381 atagccgcaa agccctggtc gggaatttaa aataatcctc tattttaaga cggcataata
     4441 cttttttatg ccgtttaatt cttcgttttg ttacctgcct ctaactttgt agatctccaa
     4501 aatatattca cgttgtaaat tgtttaacgt caaatttccc atacaaagct aagggataat
     4561 gcgtagcgtt cacgtaactg gaggaatgaa atggagtttt tcaaaaagac ggctcttgcc
     4621 gcactggtta tgggttttag tggtgcagca ttggcattac ccaatatcac cattttagca
     4681 accggcggga ccattgccgg tggtggtgac tccgcaacca aatctaacta cacagcgggt
     4741 aaagttggcg tagaaaatct ggttaatgcg gtgccgcaac taaaagacat tgcgaacgtt
     4801 aaaggcgagc aggtagtgaa tatcggctcc caggacatga acgataatgt ctggctgaca
     4861 ctggcgaaaa aaattaacac cgactgcgat aaaaccgacg gcttcgtcat tacccacggt
     4921 accgacacga tggaagaaac tgcttacttc ctcgacctga cggtgaaatg cgacaaaccg
     4981 gtggtgatgg tcggcgcaat gcgcccgtcc acgtccatga gcgcagacgg tccattcaac
     5041 ctgtataacg cggtagtgac cgcagctgat aaagcctccg ctaatcgtgg cgtgctggtg
     5101 gtgatgaacg acaccgtact ggacggtcgc gatgtcacca aaaccaacac caccgacgta
     5161 gcgaccttca agtctgttaa ctacggtcct ctgggataca ttcacaacgg taagattgac
     5221 taccaacgta ccccggcacg taagcacacc agcgatacgc cattcgatgt ctctaagctg
     5281 aatgagctgc cgaaagtcgg catcgtttat aactacgcta acgcatccga tcttccggct
     5341 aaagcactgg tagatgcggg ctatgatggc atcgttagcg ctggtgtggg taatggtaac
     5401 ctgtataaat ccgtgttcga caccctggca accgccgcga aaaacggcac tgcagtagtg
     5461 cgttcttccc gcgtaccgac gggtgctacc actcaggatg ctgaagtgga tgatgcgaaa
     5521 tacggcttcg tcgcctctgg cacgctgaac ccgcaaaaag cgcgcgtcct gctgcagctg
     5581 gctctgacgc aaaccaaaga tccgcagcag atccagcaga ttttcaatca gtactaatca
     5641 cctcgtcccg gcatcgtgcc ggggcttttt cacttcagac ttacgtccat tgccaatttt
     5701 cattacccta atgataatca ccggaataaa ttattccgcg cgagggtttt cgggtgaaaa
     5761 agcaatggat tgttggcacg gcgctgctta tgttgatgac tggtaatgcc tgggcagatg
     5821 gcgaaccgcc aactgaaaat atcttaaaag atcaattcaa aaagcagtat cacggcattc
     5881 tcaagcttga tgccatca
//
LOCUS       DADLSA010000196         5852 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.197, whole genome shotgun sequence.
ACCESSION   DADLSA010000196 DADLSA010000000
VERSION     DADLSA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.197"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            163..237
                     /gene="tnaC"
                     /locus_tag="JN691_003793"
     CDS             163..237
                     /gene="tnaC"
                     /locus_tag="JN691_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HAY7245090.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            458..1873
                     /gene="tnaA"
                     /locus_tag="JN691_003794"
     CDS             458..1873
                     /gene="tnaA"
                     /locus_tag="JN691_003794"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HAY7245091.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPYIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1964..3211
                     /gene="tnaB"
                     /locus_tag="JN691_003795"
     CDS             1964..3211
                     /gene="tnaB"
                     /locus_tag="JN691_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HAY7245092.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3343..4518
                     /gene="mdtL"
                     /locus_tag="JN691_003796"
     CDS             3343..4518
                     /gene="mdtL"
                     /locus_tag="JN691_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAY7245093.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALEIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4493..5452
                     /gene="yidZ"
                     /locus_tag="JN691_003797"
     CDS             4493..5452
                     /gene="yidZ"
                     /locus_tag="JN691_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HAY7245094.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLV
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPHYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5609..>5852
                     /locus_tag="JN691_003798"
     CDS             5609..>5852
                     /locus_tag="JN691_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheinyl transferase"
                     /protein_id="HAY7245095.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFS"
BASE COUNT         1383 a         1406 c         1421 g         1642 t
ORIGIN      
        1 ttttaaacat taaaattctt acgtaattta taatctttaa aaaaagcatt taatactgct
       61 ccccgaacga ttgtgattcg attcacattt aaacaatttc agaatagaca aaaattctga
      121 gtgtaataat gtagcggcgt gtctttcgag gataagtgca ttatgaatat cttacatata
      181 tgtgtgacct caaaatggtt caatatcgac aataagattg tcgatcaccg cccttaattt
      241 acccttctgt agccatcacc agagccaaac cgattagatt caatgtgatc gattagtttg
      301 ctatatctta attttgcctt ttgcaaaggc catctctcgt ttatttactt attttagtaa
      361 atgatggtgc ttgtatatat acatggcgaa ttaatggata ttgcagatgt aatattcaca
      421 gggatcactg taattaaaat aaatgaagga ttatgtaatg gaaaacttta aacatctccc
      481 tgaaccgttc cgcattcgtg ttattgagcc agtaaaacgt actactcgcg cttatcgtga
      541 agaagcaatt attaaatccg gtatgaaccc gttcctgctg gatagcgaag atgtgtttat
      601 cgatttactg accgacagcg gcaccggggc ggtaacccaa agtatgcagg cagcgatgat
      661 gcgcggcgac gaagcctaca gcggcagccg cagctactat gcgttagccg agtcagtgaa
      721 aaatatcttt ggttatcaat atactattcc gactcaccag ggccgtggcg cagagcaaat
      781 ctatattccg gtactgatta aaaaacgcga gcaggaaaaa ggcctggatc gcagcaaaat
      841 ggtggcattc tctaactatt tctttgatac cacgcagggc catagccaga ttaacggctg
      901 taccgtgcgt aacgtctata tcaaagaagc cttcgatacg ggcgtgcgtt acgactttaa
      961 aggcaacttt gacctcgaag gattagaacg cggtattgaa gaagttggcc cgaataacgt
     1021 gccgtatatc gttgcaacca tcaccagtaa ctctgcaggt ggtcagccgg tttcactggc
     1081 aaacttaaaa gcgatgtaca gcatcgcgaa gaaatacgat attccggtgg taatggactc
     1141 cgcacgcttt gctgaaaacg cctatttcat caagcagcgt gaagcagaat acaaagactg
     1201 gaccatcgag cagatcaccc gcgaaaccta caaatatgcc gatatgctgg cgatgtccgc
     1261 caagaaagat gcgatggtgc cgatgggcgg cttgctgtgc atgaaagacg acagcttctt
     1321 tgatgtgtac accgagtgca gaaccctttg cgtggtgcag gaaggcttcc cgacatatgg
     1381 cggcctggaa ggcggcgcga tggagcgtct ggcggtaggt ctgtatgacg gcatgaatct
     1441 cgactggctg gcttatcgta tcgcgcaggt acagtatctg gtcgatggtc tggaagagat
     1501 tggcgttgtc tgccagcagg cgggcggtca cgcggcattc gttgatgccg gtaaactgct
     1561 gccgtatatc ccggcagacc agttcccggc acaggcgctg gcgtgcgagc tgtataaagt
     1621 cgccggtatc cgtgcggtag aaattggctc tttcctgtta ggccgcgatc cgaaaaccgg
     1681 taaacaactg ccatgcccgg ctgaactgct gcgtttaacc attccgcgcg caacatatac
     1741 tcaaacacat atggacttca ttattgaagc ctttaaacat gtaaaagaga acgcggcgaa
     1801 tattaaagga ttaaccttta cctacgaacc aaaagtattg cgtcacttca ccgcaaaact
     1861 gaaagaagtt taattaatac tacagagtgg ctataaggat gttagccact ctcttaccct
     1921 acatcctcaa taacaaaaat agccttcctc taaaggtggc atcatgactg atcaagctga
     1981 aaaaaagcac tctgcatttt ggggtgttat ggttatagca ggtacagtaa ttggtggagg
     2041 tatgtttgct ttacctgttg atcttgccgg tgcctggttt ttctggggtg cctttatcct
     2101 tatcattgcc tggttttcaa tgcttcattc cgggttattg ttattagaag caaatttaaa
     2161 ttatcctgtc ggctccagtt ttaacaccat caccaaagat ttaataggta acacctggaa
     2221 cattatcagc ggtattaccg ttgccttcgt tctctatatc ctcacttatg cctatatctc
     2281 tgctaatggt gcgatcatta gtgaaacgat atcaatgaat ttgggctatc acgctaatcc
     2341 acgtattgtc gggatctgca cagccatttt cgttgccagc gtattgtgga taagttcgtt
     2401 agccgccagt cgcattacct cattgttcct cgggctgaag attatctcct ttgtgatcgt
     2461 gtttggttct ttcttcttcc aggtcgatta ctccatcctg cgcgatgcca ccagcaccac
     2521 tgcgggaacg tcttacttcc cgtatatctt tatggctttg ccggtgtgtc tggcgtcatt
     2581 tggtttccac ggcaatattc ccagcctgat tatttgctat ggcaaacgca aagataagtt
     2641 aatcaaaagc gtggtatttg gttcgctgct ggcgctggtg atttatctct tctggctcta
     2701 ttgcacgatg gggaatattc cgcgcgaaag ctttaaggcg ataatctcct caggcggcaa
     2761 cgttgattcg ctggtgaaat cgttcctcgg caccaaacag cacggcatta tcgagttttg
     2821 cctgctggtg ttctctaact tagctgttgc cagttcgttc tttggtgtca cgctggggtt
     2881 gttcgattat ctggcggacc tgtttaagat tgataactcc cacggcgggc gtttcaaaac
     2941 cgtgctgtta accttcctgc cacctgcgtt gttgtatctg atcttcccga acggctttat
     3001 ttacgggatc ggcggtgccg ggctgtgcgc caccatctgg gcggtcatta ttcccgcagt
     3061 gcttgcaatc aaagctcgca agaagtttcc caatcagatg ttcacggtct ggggcggcaa
     3121 tcttattccg gcgattgtca ttctctttgg tataaccgtg attttgtgct ggttcggcaa
     3181 cgtctttaac gtgttaccta aatttggcta aatccttcaa gaagccagcc attcgctggc
     3241 ttcttgcctc tcaggaaatc acttatgtcc aaatggcaac tcgccttatc ctccttcacc
     3301 acgtatgctt tgcgtcacct tactatcagg acgctttagc ccatgtcccg ctttttgatt
     3361 tgtagttttg ccctggtttt actttatccc gccgggattg atatgtacct cgttggttta
     3421 ccgcgcatcg ccgccgatct caatgccagc gaagcgcagt tgcatattgc gttctccgta
     3481 tatctggcgg ggatggcagc tgcgatgcta tttgccggta aagtggccga tcgttcaggg
     3541 agaaagccgg tcgccatacc cggcgcggcg ctatttatta ttgcctcggt gttctgttca
     3601 ctggctgaaa ccagcacgtt atttcttgca ggccgatttc tacaggggtt gggcgcaggc
     3661 tgttgttacg tggtggcgtt cgccattttg cgcgacacgc tggatgatcg acgtcgggct
     3721 aaagtgctgt cattactcaa cggtattacc tgcatcattc cggtgttagc gccagtgctc
     3781 ggacatctga ttatgcttaa attcccgtgg cagagtctgt tctgggcgat ggcaatgatg
     3841 ggcatcgcgg tactgatgtt gtctttgttt attttaaaag aaacgcgccc agcgtccccc
     3901 gctgcttcgg acaaaccacg agaaaatagc gagtcgctgc ttaatcgttt tttcctcagc
     3961 cgtgttgtta tcaccaccct cagcgtttcg gtgatcctca ctttcgtcaa tacatcgccg
     4021 gtattgctga tggaaatcat ggggtttgag cgcggagaat acgccaccat tatggcgctg
     4081 accgctggcg tcagcatgac cttttcattc tcgacgccat ttgcgctgga aatttttaag
     4141 ccacgtacgt tgatgatcac ctcgcaggtg ttattccttg cggcggggat cacccttgcc
     4201 gtttcacctt cccatgcggt ttctctgttt ggtatcacgc tgatttgcgc cggtttctcg
     4261 gtaggttttg gcgtagcgat gagtcaggcg ttaggaccat tttcattacg cgcgggcgta
     4321 gccagctcga ccttaggtat tgcgcaggtt tgcggttcgt cactgtggat ttggctggca
     4381 gcggtggttg gtatcggcgc atggaatatg ctgatcggga ttctgattgc ctgtagcata
     4441 gtgagcctgt tgctgattat gttcgtcgcg cctggacgcc ccgttgccgc tcatgaagaa
     4501 atccatcacc acgcttgatc tcaatctgct gctctgtctg caactgctga tgcaggagcg
     4561 cagcgtgacc aaagcggcga agcggatgaa cgtgacccct tcagcggtga gtaagtcgct
     4621 ggtaaagtta agagcgtggt ttgacgaccc gctctttgtg aactcaccgc tggggctgtc
     4681 gcccacaccg ctaatggtca gcatggagca aaatctggcg gaatggatgc aaatgagcaa
     4741 tctgctgctg gataaaccgc accaccagac accgcgcggc ctgaagtttg agttggcggc
     4801 ggaatcgccg ctgatgatga tcatgcttaa tgcgctgtcg aaacggatct accaacgtta
     4861 cccgcaggcg accatcaaac tacgtaactg ggattacgat tccttagatg ccattactcg
     4921 tggtgaagtg gatattggtt tttccggtcg cgaaagccat cctcgctcgc gggagctgtt
     4981 aagctcgcta ccgttagcca ttgattatga agtgctgttt agtgatgtac cctgcgtctg
     5041 gttacgcaaa gatcatccgg cactgcatga aacgtggaat ctggacacct ttttacgtta
     5101 tccgcatatc agcatttgct gggaacagag cgacacctgg gcgctggaca atgtgttaca
     5161 ggagctgggg cgcgaacgca cgattgctat gagcctgccg gaattcgagc agtcactgtt
     5221 tatggcagcg caacccgaca atctgctact ggcgaccgcg ccgcactact gtcagtacta
     5281 caatcaactc catcaactgc cgttggttgc tcttcctctc ccgtttgacg aaagccagca
     5341 aaaaaagctg gaagttcctt ttaccctgct gtggcataaa cggaacagcc ataatccgaa
     5401 gatcgtctgg ttacgggaaa ccattaaaaa cctttacgcg tcgatggcat aaccgaatcg
     5461 tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag ttgtaataag cttgtctgaa
     5521 tcgaactttt agccgcttta gtctgtccat cattccagta aatgattact cttgtattca
     5581 taatggacca ttaagcatgg agcgaaaaat ggcgactcac tttgcccgag ggattttaac
     5641 ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt catcaagaag cccgaaacat
     5701 tccccctcat cgtcaaagcc gttttctggc gtccagaggt ttactcgcag aactgatgtt
     5761 catgctgtat ggcattggcg aattgccgga aatcgtcacc ctgccgaaag gtaaaccggt
     5821 tttcagtgat aaaaatttgc cttcgttttc ta
//
LOCUS       DADLSA010000197         5848 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.198, whole genome shotgun sequence.
ACCESSION   DADLSA010000197 DADLSA010000000
VERSION     DADLSA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.198"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..956)
                     /locus_tag="JN691_003799"
     CDS             complement(<1..956)
                     /locus_tag="JN691_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY7245096.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGS"
     gene            1203..1829
                     /locus_tag="JN691_003800"
     CDS             1203..1829
                     /locus_tag="JN691_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY7245097.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1826..2941)
                     /locus_tag="JN691_003801"
     CDS             complement(1826..2941)
                     /locus_tag="JN691_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY7245098.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(3052..3435)
                     /gene="bssR"
                     /locus_tag="JN691_003802"
     CDS             complement(3052..3435)
                     /gene="bssR"
                     /locus_tag="JN691_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAY7245099.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSFTLSIQSLQKHAMLEKA"
     gene            3648..4973
                     /gene="rimO"
                     /locus_tag="JN691_003803"
     CDS             3648..4973
                     /gene="rimO"
                     /locus_tag="JN691_003803"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAY7245100.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5020..>5848)
                     /locus_tag="JN691_003804"
     CDS             complement(5020..>5848)
                     /locus_tag="JN691_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7245101.1"
                     /translation="SLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYL
                     PTGLYVFAYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNH
                     FERVLDGGLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFD
                     VDKFKNINDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEEL
                     LSILKEIVHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
BASE COUNT         1482 a         1519 c         1390 g         1457 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgctcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atcggccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcgggatc tagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaaatac gttatatcgc cgtatgataa gttcggtgat
     1021 taataacgca cacacaaact taaagaaacc gtaagttggt aaagttttta aagtttatgc
     1081 aatatcctgc cgcttcgctg cttctggtgt catttttttg ccagctgttg cttaaaagtt
     1141 aactttactt caccatcgga gtcctgttca gtctgtggct gcctgtcgga aaaggggtga
     1201 gtatgattac gctgtggggt cggaataatt caactaacgt aaaaaaagta ttgctgacgc
     1261 tcgaagaact ggaactacct tatgagcaaa ttctcgcggg ccgtgagttt gggataaatc
     1321 acgatgctga ttttctggcg atgaacccta acgggctggt gccgttgttg cgtgacgacg
     1381 aaagtgatct cattctttgg gaatcaaacg ccattgtccg ctatctggcg gcacagtacg
     1441 ggcaaaaacg cctgtggatc gactcaccgg cacgtcgtgc ggaagcggaa aaatggatgg
     1501 actgggcaaa ccagacgctc agtaatgctc atcgcgggat cctgatggga ttagtcagaa
     1561 caccaccgga agagcgcgat caggccgcca ttgatgccag ttgcaaagag tgcgacgccc
     1621 tgtttgccct gctcgatgcg gaactggcaa aagtaaaatg gttctctggc gacgagtttg
     1681 gtgtgggcga tatcgctatc gcaccgttca tctacaattt gtttaacgtt ggcctgacct
     1741 ggacaccgcg tccaaatctg caacgctggt atcagcaact cactgaacgc cccgcagtgc
     1801 gcaaagtggt gatgattccc gttagctaat tgcgtgggct aactttaagt aattccccac
     1861 tggactcgtc ggtgagaacg tataaataac cgtcgggtcc agtgcgaaca tcacgaattc
     1921 gctgccctct gtccgttaaa atacggccat cttctgtcac tttgtcgccg ttgacgctca
     1981 tcacaatgac atctttatct ttcagcgcgc caataaataa tttttgctgc cactggggga
     2041 atttatcgct gttatagaag gccatgccgc tcacagcggg cgaatctttc cagtaaaaaa
     2101 caggttgctc ggtcccggcg acgatctccc ctttcgcttc cggtatctta aagcctgaat
     2161 agttgattcc ccaggttgcc agcggccagc cgtagttttt gcctttttgc gggatattaa
     2221 tttcatcacc accgcgcggg ccatgttcat tcagccacag tgcattactc cacggattca
     2281 tcgccattcc ttgcggatta cgaatgccat aagaccagat ctcggcgcgc gcaccggatt
     2341 cctttataaa aggattatca tccgggattt cgccctggtc ggtcagacgc accagtttgc
     2401 cctgtaattt atccagatcc tgcgccgtcg ggcgctgatt gttttcgccc agagcaataa
     2461 aaagataacc tttaccgtcg aataccagcc gcccgccaaa atggttgccg gtagacagtt
     2521 ttggcatctg gcggaagacg gtgcggaagt cggtcacttt tgagagatca tcacttaagc
     2581 ggccataacc cacagcagtt ccggctttgc catcatcgcc aacttcggaa taacttaacc
     2641 agatgcggcg agactgagca aaatcaggcg ctaaaaccac gtccagcagg ccgccctgcc
     2701 cgtgcgccca aacgtccgga actccggaaa gcggcgcaga taatcctttt cctgcttgcc
     2761 agtgacgcaa ctcgccgccg cgcagagtga ttaacatacc gtgattatcg ggtaaaaagg
     2821 ccagtgccca gggatggtcg agtttgtctt gcagtacttc gacatttacc gttgcaggag
     2881 ccgcccagag agcggaagaa agacaaataa ggggcacaag gaaaaaggat tgtcgatgca
     2941 taacgcctcc ggattgactc tggcttaaag cgtagtcagt ggaggagata acaaattcat
     3001 ttttacaaaa acttaaacat gaagggggag acgctttctc ccccttagtt ttcaggcctt
     3061 ctcaagcatg gcgtgcttct gcaggctctg gatactcagc gtaaagctca tcagacaatt
     3121 ttcaagctta tcggcgttga cggtaataac agtcgggcaa tcatggtgcc cactcatcaa
     3181 acatactgcg gctgtcgcta atgcttcttc agcatgatga agagcactcc actcttcctg
     3241 atccagatga agattcaacc gcagcgattt atcgtgcagt tcgcgattca gtttaaaaaa
     3301 gttatctcgt agatgattgc tttcgctgac ggacatgtat ccttttgcct tcctcagttg
     3361 cacgtatgca gcgaggtcaa cgcgtgcgtc gatcaaccgg ttcagcagat cgattcgctg
     3421 tctgtcaacg aacatacttc gatcctcctc tttccagcct tgttgcaatt aaatagtatg
     3481 gctatttttt gagcaaaatg ctgtccagga tcaattcttc acgccgaatt aacaaaattt
     3541 acatttcctt cgcctccgcc agcgaatgag attgcgatac aaattttatc cagaactctg
     3601 tagaatccct gccctgacct ttcctgtaac tgaatatttt ttaagctatg agcaaagtaa
     3661 ctccccagcc gaaaatcggc tttgtttccc ttggctgtcc gaaaaacctt gtcgattcag
     3721 agcgtattct caccgaactc cgcactgaag gttatgacgt ggtaccgagc tatgacgatg
     3781 cggacatggt gatcgtcaac acctgcggct ttattgacag cgcggtacaa gaatcactgg
     3841 aagccattgg tgaagcgttg aatgaaaacg gcaaggtaat tgtgaccggt tgtctggggg
     3901 caaaagaaga tcagatccgc gaagtccacc cgaaagtgct ggaaatcacc gggcctcata
     3961 gctatgagca ggttctggag cacgttcatc actacgtgcc aaaaccgaaa cacaacccat
     4021 tcctgagcct ggtgccagaa caaggtgtga aactgacgcc gcgtcattat gcctatctga
     4081 aaatttcgga aggctgtaat caccgctgca ccttctgcat tattccgtct atgcgcggcg
     4141 acctggtgag ccgtccgatt ggcgaagtgt taagtgaagc gaaacgtctg gtagatgcgg
     4201 gcgttaaaga gattctggtg atctcgcagg atacttccgc ctatggcgtt gatgttaaac
     4261 atcgtactgg cttccacaac ggcgagccgg taaaaaccag catggtcagc ctgtgcgaac
     4321 agttatcgaa actggggatc tggacacgtc tgcactacgt ttacccttat ccgcatgtgg
     4381 acgacgtcat cccactgatg gcagaaggca aaatcctgcc gtatctggac attccgttgc
     4441 agcacgccag cccgcgcatt ctcaaactga tgaagcgtcc gggttctgta gatcgccaac
     4501 tggcgcgcat caaacagtgg cgcgaaatct gcccggaact gaccctacgc tcaaccttta
     4561 ttgtcggctt ccctggcgag acggaagaag atttccagat gctactcgac ttcctgaaag
     4621 aagcacgtct ggatcgcgtt ggctgcttta aatacagccc ggttgaaggt gcagacgcca
     4681 atgccctgcc tgaccaggtt ccggaagaag tgaaagaaga acgctggaac cgtttcatgc
     4741 agctgcagca gcagatttcc gccgagcgcc tgcaagagaa agtgggccgt gaaattctgg
     4801 tgattatcga cgaagtggac gaagaaggcg cgattggtcg cagcatggca gatgcaccgg
     4861 aaatcgacgg cgcggtttat ctcaatggtg aaaccaacgt taagccgggt gatatcctgc
     4921 gtgtgaaagt cgagcacgcc gatgagtacg atttgtgggg tagccgggtt taattaattc
     4981 aggccgggca aaatcatttg tccggcctgc gaataatgat tactgccagc taatctgtgc
     5041 cctgccgttt ttcttactct tatacagccg ctcatccgcc agcattttca agcgttcgac
     5101 ggtagtacat tcatttgaat gcgctacacc gatactggtg cttaaattaa tgcttcccac
     5161 ctggaaatga acgatttctt ttagtatcga tagcaactct tccagcgtgc cgccagtata
     5221 aatcaccgca aattcgtcac ctccgaagcg gtagacttta cctttatcta ccgggatctg
     5281 tgatttaagg attttcacca ctttcatcaa cacttcatcg cccagcaaat gaccgaaggt
     5341 gtcgttaatg tttttgaatt tatcgacgtc aaacagacac aaataaccgc ctttttccgg
     5401 cagcgctttt aaatcttcgt caaaggattt tctgcccatt gctttggtca ggtcgtccag
     5461 gaacgccgag ttatacatac tgtagagttt tttgacatcg gcagccgaaa agaaaagccc
     5521 gccatccagt acgcgttcaa aatggttatt aatgatggct tctttctcca caataccacg
     5581 attaatcact ctgcgtacca gatagaggca actggcaccc gatatcactg ccaccaatgc
     5641 ggcaaagaaa ataatcaatg tacaaacccg caaataaaca tcttttttat acgcaaatac
     5701 gtacaatccg gtcggcaaat aggcgaagtg ctccatcgct tccgaaacgg tgaagatggc
     5761 gtttttagca ttatattcat caccggtcac cggtttaccg ttcttaaata gagagaacat
     5821 caggttctcg ctgtccatcg ccagcgat
//
LOCUS       DADLSA010000198         5788 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.199, whole genome shotgun sequence.
ACCESSION   DADLSA010000198 DADLSA010000000
VERSION     DADLSA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.199"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(2..472)
                     /locus_tag="JN691_003805"
     CDS             complement(2..472)
                     /locus_tag="JN691_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAY7245102.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTKQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(519..1238)
                     /gene="yehT"
                     /locus_tag="JN691_003806"
     CDS             complement(519..1238)
                     /gene="yehT"
                     /locus_tag="JN691_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAY7245103.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1235..2920)
                     /gene="btsS"
                     /locus_tag="JN691_003807"
     CDS             complement(1235..2920)
                     /gene="btsS"
                     /locus_tag="JN691_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAY7245104.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVHLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3142..3207
                     /locus_tag="JN691_003808"
                     /pseudo
     CDS             3142..3207
                     /locus_tag="JN691_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            3435..3683
                     /locus_tag="JN691_003809"
     CDS             3435..3683
                     /locus_tag="JN691_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY7245105.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(3799..4068)
                     /locus_tag="JN691_003810"
     CDS             complement(3799..4068)
                     /locus_tag="JN691_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7245106.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            4125..4793
                     /locus_tag="JN691_003811"
     CDS             4125..4793
                     /locus_tag="JN691_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7245107.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            5109..>5788
                     /locus_tag="JN691_003812"
     CDS             5109..>5788
                     /locus_tag="JN691_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7245108.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT         1625 a         1399 c         1233 g         1531 t
ORIGIN      
        1 cttagctttt cttcacatgc tggcgcgctg ccagtccacg cagaaaataa cgtaaaaatt
       61 gatcgccgca ttcgcggaag tttttatgat ccggtgcgcg catcatcgct gtaatttccg
      121 gcatcgaaac gcggaactgc tgtttggtga ggatagccag aatgtcatcg gttttcagcg
      181 aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt aatgcgacgt tccggctcca
      241 gtgccggagc agactcatcc ttgccgcgtt tttcataaat caggccattg aggaatgacg
      301 acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc ttctttacgt agccagacgg
      361 cgatctgttc cgcggtggct tcgacattat ccagcgccag aatacgcacc aggtcattat
      421 tattggcttt caaaatgtag cgcacgctgc gcagaatatc gttacttagc atgaggcctt
      481 caggtgttga tgaggcaaaa agccatttta gcagtctttt acaggccaat cgcctctttt
      541 aagcttttca gataacggcg gctgaccggc acggttaaac cattacgcaa aatcaactcg
      601 gcctggccgt tatcttccaa acgaatctcc tgtaaatgcg cgaggttaac cagatactga
      661 cgatggcagc gcagtagtgg tgtacgactt tccagggtac gtaatgtcaa ttcggtaaag
      721 ccctctttcc cttcgtggct ggtaacgtag acaccgctca tccgactgct gacaaatgcc
      781 acatctttca tttgcagcaa ataaatccga ctatgccccg tacaagggat aaatttcagc
      841 gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc tgcgctcctg acgcaatcgc
      901 gccagcgttt tctccagtcg cgcttcatca attggcttca gcagataatc aaaggcatgt
      961 tcttcaaagg ctttaatagc gtattcgtca aacgcagtga gaaaaacaat atacgggcga
     1021 tgttccgggt caagcatccc caccatttcc agaccactga tgcgcggcat ctggatatcg
     1081 agaaacagca catccgggcg cagtttatgc accgcgccga tcccttccac ggcgtttgaa
     1141 cactctccaa cgatttcaat atcgctctgc tcctgcaaaa atatacgcag gttctcccgt
     1201 gctaacggtt catcatcaac aattaagact ttaatcatgc ctcgtccctc catggtagtc
     1261 gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac gcttattcca taatcatcgc
     1321 caaaccgttc acgtaaacgc ttatccacca gattcatccc cagcccactg gcattggtta
     1381 ccggttgata caaaccggca ttgtcttcga tctccagcat caaatgttgc ccctcacgtc
     1441 gggcgctgat tgccactcgc cctgtatcca gcagttgtga tgtcccatgt ttaatggcgt
     1501 tttccactat cggttgcagg gtaaacgcgg gcaattgctg ctgggataat tcttgcggaa
     1561 tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt ttcaatttgc agataagcgt
     1621 tcacatgttc aatttcgtcg gcgagagtaa caaactccga aggccgcttt aagtttttgc
     1681 ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg ttcgctgtcg cggcggatca
     1741 ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg attcacctgg gcgtgaagca
     1801 gtttgatttc tgactgggtg agcatcgctt tttgccgctc atattgcccg gcaagaatct
     1861 gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg gtttattgaa ctgaataaac
     1921 ggtttttggc ttcatacaat ttgatggtgc ccatcacacg ctgattttcg ccacgcaacg
     1981 gaattaccag cgtcgacccc agtttgcatt gcggatgcaa agagcaacga taaggtactt
     2041 cgttgccatc agcgtagacc acttcaccgg tttcaatcgc ttttaaggtg taagtcgaag
     2101 aaatcggttt gccgggtaaa tggtggtcgt caccaattcc ggtaaaggcc agcaatttct
     2161 ctcgatcggt aatcgcgact gcaccaatat ccagctcctg atacagcacc tgagccactt
     2221 tcatgctgtt cacttcgtta aacccctgtc gcaaaatgcc ttccgtcgag gctgccactt
     2281 tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa catcgcgcgt ttatcgagca
     2341 atatacgcat aaacagcgcc gcgccgacgg tattggtgac catcattggc gcagcaatat
     2401 tactcaccag atgcaccgca tcttcataag gtcgggcgat cgcaaggatg atcagcattt
     2461 gcaccatttc agcgacgaac gtgacggcac cggcggtaat ggggttaaag actttatcag
     2521 tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc gagcaatcct tcaacgatgg
     2581 ttgagatcat gcaacttagc gcggtcatgc cccccatcga atatcgatgt aagccgccgg
     2641 tcagaccaac cagcccaccg acgaccggac cgccgagtaa gccgcccatt accgcgccta
     2701 tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc aaaccaggtg cccatgatgc
     2761 agaagatgga aaagacgatg tagcagagaa atttatgcgg cagacgaacc gtgacctgca
     2821 ttaacggtat gaataatggc gttttactca ttaaccacgc aatgactaaa aaaacgcaca
     2881 tctgctgaag cagcagcaac accagattaa aatcgtacat acccgcaaac cacacttccc
     2941 tttaaaacgc gtaacataca ttgcctgcgt ttaactttct ttgaactctt gcagaaaaat
     3001 gagaattcgt gagtacgatc actcaaaatc gcctggcaaa aataaaatca ccctatagat
     3061 gcacaaaaaa cgggcaaaac tacctggttc gcaaaactgc gtctaaagtt aaactgggac
     3121 ctcgcgagca agggtgagat gatggcgctt tacacaattg gtgaagtggc gttgctttgt
     3181 gatattaatc ctgtcacgtt acgcgcgtga cgcaacattt ttaaaaatcg acgaagatcg
     3241 tccaggagcg ccgtttttca agggttggat agatgattcg tttaccatgt cgacgaactt
     3301 cgacgaattt cgccgataaa aagtgtccca tacatgcccc attgatgccc cataaacaat
     3361 atcaggaatt gacactggtt atccatacag ataaaaataa cacctgtaca taactacagt
     3421 tacaggaggg tgttatgcgt gttgagatct gtatcgctaa agaaaaaatc actaaaatgc
     3481 caaacggtgc tgtggatgcg ttaaaggaag aattaacccg acgcatcagt aaacgttatg
     3541 acgatgtaga ggtgatcgta aaagccacca gcaacgatgg cctttctgtt acacgcaccg
     3601 cagataagga ttctgcaaaa acatttgttc aggagactct gaaagatacc tgggagtctg
     3661 ctgacgagtg gtttgttcac taataaacac gtaaaatcgg taacggctgg aaatcattca
     3721 atactcgcac tatcgaaagt ttaccagcca accgcagcac gtcctgcata cgtcgtgtct
     3781 gcggtttttc tttttcgctt acattgtgtc tggttcttcc ggccactcaa tatcaggtgc
     3841 agttgatgta tcaacacggt tcagcaacac ccgatacttc ttccaggctt ccagcaacga
     3901 gttttcttcc tccgttgcga tctccagatc tacagcatcc tgaagtggcg caatatgctc
     3961 actgaatccc tggatgtaga actgtgtggt gacggtcttc cagccattcg gctcctgctg
     4021 tatcgaagca taccaggcta tttcaatatc gctatgctgc ggcagcattt aaccccttgt
     4081 aattcatcgc cataattgat ttaattcaca aataaaacta taatatggtg aaattaatga
     4141 aaaaaaacac agatgatggg gctaaaattt acacaccact taccctaaag ctttatgact
     4201 ggtgggtttt gggagtatca aatcggcttg catggggatg tcctacaaag gaacaccttc
     4261 ttccacactt tctggaacat ttaggtaaca accatctgga tattggtgtt ggaactgggt
     4321 tttaccttac tcacgtacct gagagtagtc tgatatcttt aatggatttg aacgaagcta
     4381 gcctgaacgc ggcatctaca agggctgggg aatcaaaaat taaacataaa attagccatg
     4441 atgtttttga accttatcct gcggcgttac atggtcaatt tgattccatt tccatgtttt
     4501 accttcttca ctgcctgcct ggaaatatat ctacaaaaag ctgtgtaata cgcaatgcgg
     4561 cgcaggcctt aactgatgat ggaactctat acggagccac aattcttggc gatggagttg
     4621 tgcacaatag cttcggtcaa aaactgatgc gcatttacaa tcagaaaggc atcttttcaa
     4681 acacaaaaga ttccgaagaa ggcttaacac atatactctc agagcatttc gagaatgtta
     4741 aaaccaaggt tcaaggtact gtagtaatgt tttccgcttc agggaaaaaa tagcatccaa
     4801 ccgcagcacg ttcttgctta agacgtgcta cggtttcctt tacgatttgg ggttgcgact
     4861 ttacctctat tgataatgca ttccggcaga acgttcaaat atgaacgttc gatatttaac
     4921 taaccgaaaa acaaaatata gcacaggcga gacatttacc atctaagaaa atagtatcgt
     4981 ttttttacag ttaaatcaac atcacttcct taaaatgaaa acaataaatt tcaatcctga
     5041 aacattttat ttcagtttat tatttcccag tatcacattt tatcagaata tccttctgcg
     5101 aggttattat gcttcctgta aataatcccc ccctatccac tggaaacgtc tctttttaca
     5161 gaactacatc aatcgacaat gttcacaata attatctctc cgaatgggtt gaatggacta
     5221 aaaacagcat ttccggagaa aacagggaaa ctgcttttac ccggctccaa ttatgtctgg
     5281 agaacagtga aacatcgttg gacttatctt gtttaggtct cagatctcta ccacgattgc
     5341 ctgacaatct tgatgaaatt aatgtaagca ataaccaact atcaatgctc cccgagctac
     5401 caagggcatt gaaagagctg aatgcaagca gtaatcaatt atctgcactt cctgaattac
     5461 cagtgtcgct ggaatatata aatgtgagtg ataaccattt gttcgcactt cctgaattac
     5521 ctgcgtcact agaatatatt aatgtaagtg acaatcacct gtctgtactt ccgaggttac
     5581 caatgtcatt ggaattactt gatgcagcca gaaatgcttt ggaagtaata ccagattttc
     5641 cagaaagaga tgatcatatt ataagaatat tctggcttaa tcagaaccgg atcacggcaa
     5701 ttccggaaag catacttggc ctcagttctg atagcgttgt caatcttaga gaaaatcaac
     5761 tatctcccag aataatgcaa actttgtt
//
LOCUS       DADLSA010000199         5787 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.200, whole genome shotgun sequence.
ACCESSION   DADLSA010000199 DADLSA010000000
VERSION     DADLSA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.200"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(553..858)
                     /locus_tag="JN691_003813"
     CDS             complement(553..858)
                     /locus_tag="JN691_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245109.1"
                     /translation="MGSKIAGVVFLYFAVWGMASVIELQYWRNADTYPVWLTKHLRIS
                     PENNDKSLIRIEMETFDKVCGDKRGLLAVTQKQNGMFMRCDDSPGFFSWFSGVYKVN"
     gene            complement(912..1163)
                     /locus_tag="JN691_003814"
     CDS             complement(912..1163)
                     /locus_tag="JN691_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004182043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAY7245110.1"
                     /translation="MTNIILCDVTASVSELKNDPVATASARGGYPVEIIDRNRPVFYC
                     VPAALYEQMLDELDEKDLVQTITERQNQPLREVDLNQYL"
     gene            complement(1242..2513)
                     /locus_tag="JN691_003815"
     CDS             complement(1242..2513)
                     /locus_tag="JN691_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAY7245111.1"
                     /translation="MFALADVNSFYASCEKVFRPDLRGKPVVVLSNNDGCVIARSAEA
                     KRLGIKMGTPWFQLKEAQFPEKLYVFSSNYELYASLSNRVVALLEELSPRVEQYSIDE
                     CFLDARGISHCMDLEDFGRQLRGHVLSGTGLTIGVGFGATKTLAKSAQWASKEWPQFS
                     GVLALSPENPRRTAKLLSLQPVEEIWGVGNRIAKKLHVMGITTALQLSLTNPTFIRKN
                     FNVVLERTVRELNGESCISLEEAPPPKQQIVCSRSFGQRITTYGEMRQAVCQYAERAA
                     EKLRGERQYCRHISTFIKTSPFAVNEPYYGNVATEKLNTPTRDTRDIIAAAVRSLDRI
                     WLDGHRYAKAGIMLNDFSPNGVAQLNLFDDVQPRPHSDALMKVLDGINHSGLGKVWFA
                     GRGIAPDWQMKREMLSPAYTTRWKELPVARF"
     gene            complement(2513..2938)
                     /gene="umuD"
                     /locus_tag="JN691_003816"
     CDS             complement(2513..2938)
                     /gene="umuD"
                     /locus_tag="JN691_003816"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY7245112.1"
                     /translation="MKFIPAVSEASIVKIPLFTERCPAGFPSPAANYTESELDLNEYC
                     IHRRHSTYFVRAIGNSMTDIGLYSGDLMVVDKAEQPRHGDIVIAEIEGEFTVKRLLLT
                     PRPALQAMNPDFPSLYPDPETLQIFGVVTAFIHKTRRAD"
     gene            3039..4259
                     /locus_tag="JN691_003817"
                     /pseudo
     CDS             3039..4259
                     /locus_tag="JN691_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISKox3 family transposase"
     gene            4714..5679
                     /locus_tag="JN691_003818"
     CDS             4714..5679
                     /locus_tag="JN691_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HAY7245113.1"
                     /translation="MTMNLHTQTTAPKASQATSVSPLEQSGSSKTKFSRGKSKARAGA
                     VKTDLYQTVTDSIIAALESGVKPWVCPWVRNGAAVGLPANFSTGTAYSGINIMLLWCS
                     AAKQGFQDERWLTYKQAQELGGQVRKGEHGTTAIFYKTLEKEDEDGEIEKIPMLKAFT
                     VFNVEQIDGLSIESVPQPVAGFDPLPQAEALMIRSGAKITEQGVKAFYRPATDEIVLP
                     ERFRFADAANFYATGLHELVHWTGAASRLNREKGNKFGSEAYAFEELIAELGSAFLLA
                     DLGITGEVQHESYIASWLKALKGDKRYIFKAAAAASKAHRWLMDC"
BASE COUNT         1372 a         1536 c         1580 g         1299 t
ORIGIN      
        1 ccacggtgcc acctgtatcg ccggtcaggt tatctgcctg ggaccacggt cccacctgca
       61 tcgtcggcac attattagtt agggacaacg gttcaatcaa tgcctgtcac gaacaggtat
      121 tggcttgtcg ccagcaaccc gttgagcgga gtaatcacgc agcaggacgg ctgttacagg
      181 cgatggtgaa agctctgttg ggatctgaac gaagcatgtt gagtgaaatt tgcaaaattt
      241 cgcagagtat gcggcttcat ctttaaattt ccgccagcat ttttaaagcc agctaccgac
      301 gctgcgcgtt ggccggccct tcgtccggcg ggtaaccagt accggccgat cttacgaccc
      361 tggatgaaca tcatcccgtc agcagcgtct ccactgtgat gtcgtttttc acactgtccg
      421 atcacaaaat agaacgggca gggacaacat atggtaggaa aagcagctgc cagagacaca
      481 atatattgat tacccacagt aacgggggat aaccatgtgg gtaacatatt gaaagtgcaa
      541 cacgttatct gattaattga ctttgtaaac gccgctgaac catgaaaaaa aaccggggga
      601 atcatcacag cgcataaaca taccgttttg tttttgggtg acggcaagga ggcccctttt
      661 atcaccacat accttgtcaa aggtttccat ttctatcctt atcaggctct tgtcgttatt
      721 ttcaggagag atccggagat gttttgtcag ccagacagga taggtatcag cgttacgcca
      781 gtactgcaat tcaatcactg aagccattcc ccagacagcg aagtataaga aaaccacacc
      841 tgcgattttg ctacccatat tgttccttaa cattggctta atacattcct tgctcctcgc
      901 accggagcac atcacagata ctgattgaga tccacttcac gcagcggctg gttttgacgt
      961 tctgttatcg tttgcaccag gtctttttcg tccagctcat cgagcatttg ttcatacaaa
     1021 gccgcgggca cacaataaaa aacagggcgg ttacggtcaa ttatctcgac cgggtagccg
     1081 ccgcgggcac tggcggtggc caccggatcg tttttaagtt cgctgacgct ggcagtcaca
     1141 tcacacagaa tgatatttgt catggttccc ccctgagaca agtcacctgt atgatcggta
     1201 aagtaagctc tggcggcaag tcccttctgt catttcaacg gtcagaaacg ggcaaccggc
     1261 agctccttcc agcgtgtggt atatgccggt gacagcatct cccgtttcat ctgccagtcc
     1321 ggcgcaatac cccgtccggc aaaccagact ttcccgagtc cggaatgatt aatgccgtcg
     1381 agtaccttca tcagcgcatc gctgtgcggc cgtggctgca cgtcatcgaa cagattcagc
     1441 tgcgcgacgc cgttcggact gaaatcattg agcattatcc ccgcttttgc gtagcggtgg
     1501 ccatcgagcc atatcctgtc cagagacctg accgctgccg cgataatgtc ccgggtgtca
     1561 cgggtagggg tgttcagttt ttccgtggcc acgttaccgt aatacggctc attaaccgca
     1621 aaaggggatg tcttgatgaa ggtgctgata tgccggcagt actgtcgctc accgcgcaac
     1681 ttttccgccg cgcgctcggc atactggcag acggcctggc gcatttctcc gtaggtggtg
     1741 atccgctgcc cgaagctgcg actgcagaca atctgctgtt tcggcggcgg cgcttcctcc
     1801 agcgaaatgc aactctcgcc gttcagctca cgtaccgtgc gctccagcac tacgttgaag
     1861 tttttccgga taaatgtagg gttcgtcagc gacagctgca gggcggtggt gatccccata
     1921 acgtgcagct ttttcgctat ccggttcccc acgccccaga tttcttccac cggctgcagg
     1981 ctgagtaatt ttgccgttct gcgtggattt tcgggcgaca gcgccagcac cccactgaat
     2041 tgtggccatt cctttgacgc ccactgcgcc gatttggcca gtgtttttgt ggcgccgaag
     2101 ccgacaccga tcgtcagtcc ggttccactg agaacatgcc cacgcagctg cctgccaaaa
     2161 tcctccagat ccatacagtg gctaattccc cgggcatcca ggaaacattc gtcaattgag
     2221 tattgttcga cgcggggcga cagctcttcc agcagggcga ccacacggtt actcaggctc
     2281 gcgtagagct cgtagttgct ggaaaaaacg tacagttttt caggaaactg cgcctctttg
     2341 agctggaacc agggggttcc catctttata cccagccgtt tggcttcagc actccgggca
     2401 ataacgcagc catcgttgtt actgagaacg acgacaggtt tgccgcgcaa atccggcctg
     2461 aaaaccttct cgcaactggc gtagaaggag ttgacgtctg ccagggcgaa catcagtctg
     2521 ccctccgggt tttatgaatg aaggccgtca ccacgccgaa aatttgcaga gtttccggat
     2581 ccgggtacag ggaagggaag tccgggttca tcgcctgcag ggctggccgc ggcgtcagaa
     2641 gcaggcgttt taccgtaaat tcaccctcaa tttccgcgat aacaatatcc ccgtgccgcg
     2701 gctgttctgc tttatccacg accatcagat cgccggaata gaggccaata tcagtcattg
     2761 agttgccaat cgctctgaca aaataggtgg agtggcgccg gtgaatgcag tactcattca
     2821 gatccagttc agactcagta tagtttgctg ccggtgacgg aaaacccgca gggcatcgct
     2881 ctgtgaacaa aggaattttt actatgctgg cttctgatac tgcaggaatg aacttcatcg
     2941 ttttttccct cgcttaaaac tgtatatgca tgggtcttcc cttgttgtgg tggctgaagg
     3001 catgataatg gtgtatttaa tcgccagagg tcaccgccat ggacgaaaag tccctctacg
     3061 ctcatattct caacctgtcc gatccgtggc aggtaaagtc cctttctctc gatgaaaatg
     3121 ccggttctgt tactgtcact attgagatcg ctgaaaacac ccggctagcc tgtccgacct
     3181 gcggtaaatc ctgttctgtt cacgatcacc gtcatcgtaa atggcgccat cttgatacct
     3241 gccagttcac cactattgtt gaagccgatg ttccacgaat tatgtgtccg gagcatggct
     3301 gcctgacgtt gcctgttccg tgggctggcc ccggaagccg gtatacgttg ctattcgaat
     3361 cgttcgttct ctcatggctg aaaatcagca ccgttgatgc tgtcaggaag caacttaagc
     3421 tcagttggaa tgcggttgac ggcattatga cccgggcagt taagcgaggt cttgcccgga
     3481 taaaaaagcc attatccgcc cgtcatatga atgtggatga ggtcgccttt aaaaaaggac
     3541 atcgttacat aacggtgatc tccgatcgcg atggtcgggc gctggcctta acggatgatc
     3601 gcggcacaga gagtcttgcc ggctatcttc gcacgctcac tgatgggcag ttgctggcta
     3661 tcaaaacgct ctcaatggac atgaacgcgg gctatataag agcagcgcgt atccacttac
     3721 ccagtgcggt tgagaaaatc gcctttgacc gcttccatgt ggcgaagcaa ctgggcgagg
     3781 tagttgataa aacccgtcag aatgaacatc cgcacctccc tgttgaaagc cgacaccagg
     3841 caaaaggaac ccgcttcctg tggcagtaca gcgataagtg gatgaccgaa tcccggcagg
     3901 aaaagctgat gtggctgcgt gcacagatga agctgacgag ccagtgctgg gcgctgaaag
     3961 agctggcaaa ggatatctgg aacaggccat ggagcgagga aagacggagt gactggcaga
     4021 gatggttggc gctggcggct aacagtgacg ttcccatgat gaaaaatgcc gcgaaaacga
     4081 taggaaaaag gctgtagggg atcctgaatg cgatgcgaca cagtgtctca aacggaaatg
     4141 cggaggcact taacagcaag atcaggctgc tgaggataaa agccagggga taccgaaacc
     4201 gggagcgctt taaactgggg gtgatgttcc actacggaaa gctgaatatg gcgttctgag
     4261 ccttcccacc atgatcgggg aagacccata tgcatacagt ataaggggcg gtgaaatttc
     4321 tgcaagtgat ggggggatag ccggtcagaa cgtccccgaa aacaaaccgc ccccggtttc
     4381 aggaagggcg aactgaccgg cagaggcagt gtaacaccgc atgtcacaaa ggcagcactg
     4441 acgtcgtcag tgcttttttt acccgtcctc ccggacgctg aagggcagtt tcacccgtgc
     4501 agctaaaggg gcggtaagtc cgggtgtgca catcgtcatt ttctcctgtg atgcctttct
     4561 cctgcgtatc agcttccggt aatcgtcccc cgtcacaacc tggacagcaa gggtaaatgg
     4621 cacgctgcgc gcccttgctg accagaacga ctccgggcag gccgaaccgt caggcgatac
     4681 gaagacgaaa ttcacactga cggagaaaca gccatgacga tgaacctgca cactcagaca
     4741 accgccccta aagcttcaca ggcgaccagc gtatccccgc tggagcagtc aggctcctca
     4801 aaaacgaaat tttccagggg caaaagcaaa gcccgtgctg gcgcagtcaa aacagacctt
     4861 taccagacag taacggacag catcattgca gccctggaaa gcggcgttaa gccgtgggtg
     4921 tgtccgtggg ttcgcaacgg cgcagcggtg ggattaccgg ccaacttcag caccggcacg
     4981 gcatacagcg gaattaatat catgctgctg tggtgtagtg cggcaaaaca gggttttcag
     5041 gacgagcgct ggctgaccta caaacaggcg caggagctgg gcggccaggt tcgtaaaggt
     5101 gaacacggca cgacggccat cttttacaag acgctggaga aagaggacga ggacggggaa
     5161 atcgaaaaaa tcccgatgct gaaagccttt accgtgttta acgtggagca aatcgacggc
     5221 ctgagcatcg agagcgtgcc gcagccggtt gccggtttcg atccgctgcc gcaggcggaa
     5281 gcactcatga tccggagcgg ggcaaaaatc accgagcagg gcgtaaaggc gttctatcgt
     5341 ccggccaccg atgaaatcgt cctgccggag cggttccgct tcgcggacgc ggcgaacttc
     5401 tacgccaccg gcctgcatga gctggttcac tggacgggtg cagcgtcccg cctcaatcgt
     5461 gaaaaaggca ataagtttgg ctctgaggcg tatgcgtttg aggaactgat cgccgagctg
     5521 ggcagcgcgt ttctgttggc ggatttgggg attacgggag aggtgcagca tgaaagctat
     5581 atcgcgtcct ggctgaaagc gctgaaggga gacaagcgct acatctttaa agcggcggca
     5641 gcggcttcaa aagcgcaccg ctggctgatg gactgctgac agctggagcc ctgcggtgca
     5701 gggcttcaga atttttgaaa gagagagaat cagggaggga attatataag tcactgtttt
     5761 ttagaattct gtaacgcggc cggcctc
//
LOCUS       DADLSA010000200         5771 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.201, whole genome shotgun sequence.
ACCESSION   DADLSA010000200 DADLSA010000000
VERSION     DADLSA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.201"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(1..391)
                     /locus_tag="JN691_003819"
                     /pseudo
     CDS             complement(1..391)
                     /locus_tag="JN691_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(577..3885)
                     /gene="icsA"
                     /locus_tag="JN691_003820"
                     /gene_synonym="virG"
     CDS             complement(577..3885)
                     /gene="icsA"
                     /locus_tag="JN691_003820"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HAY7245114.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPVAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINTDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSMINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKDRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4414..5615
                     /gene="virA"
                     /locus_tag="JN691_003821"
                     /pseudo
     CDS             4414..5615
                     /gene="virA"
                     /locus_tag="JN691_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
BASE COUNT         1783 a         1310 c          955 g         1723 t
ORIGIN      
        1 aggctgtacg cttccccatg tgatttgcct cgccacttca ccgggctcag gaccccgtag
       61 gatcttcacc gaaaaaaatg catcaccagt tgctttatta tttgttttag gtagacctgt
      121 tactaattta tacctactct gaccaagtct ggataagaaa ggcacacctg cacatttcta
      181 cttggaaatg ttttctttta ccattttgct caaaaatttc aacatccaat attcctcgta
      241 cagaactact caagtcctga atagaaaaag ttccagctgg tacagtagag tcatatatta
      301 ctcgaccttg ttgagataca acaacacgtg cattagtttc tgcaatacca gtaatctgag
      361 gcgcataacc acgaatattt ggcggtagca ttcgttcatc gctttctaat gcaattcctg
      421 tgtaacgcca agcaacagcg cattctggcg catgatctgg taaacacgtt tggcattgat
      481 cgcaggcata ccatcatgtg cacaaaaacc caccgagctt ttacacggtg ggtcccagag
      541 aaatgcagga catcaacacg ccctgcattt ttattatcag aaggtatatt tcacacccaa
      601 aataccttgg gtgtctctgt aactgttatt acccaattgc tgagatacgt ttccccagat
      661 gcttaggtta tctgcaagac gcccccccac accagttttg agttcaatca cattacggtt
      721 gcctatctgg tgatttgaca tgccattcat aattacacca tattgctttg agctccattt
      781 ccaattcact tccaaaaatg gttcgaactt acgaagagtc tccttatcga tacaactctg
      841 actaattaag gatgtacgca atcccaattt tgcttgaata ttattgaccc cagaggatgt
      901 gaccatagtg ccattgtgtt caatatagtc gtttgctttt accccatgcc tagttatctg
      961 gacttgtgga ttgagataca atgcgttgtc aatattatta tgagcagtcc agcgtatggt
     1021 tgggtagata tagccagctt ccaaagctcc tgttattcct ttgctggaat atttctcacc
     1081 agttaaccca tctcctttga ctgtattatt aaaccaaccg tactgcaacc aagcatccat
     1141 atatagacct gttcgttctt tttcatcctg aaaccaagaa ctgtacaaac cggcagtata
     1201 accgctcaca gttcctcgtg aactataact agtatactta ttatgagttt tgctatcctg
     1261 atatccataa gctcccataa tacccacatg caagccatca tgatcagtga agtttgtctt
     1321 caaaatatct ccaccaatct gataaaccat cctgttagct gtggttttta attgtctatc
     1381 agctaaatta tgctcattat gtccaccaac agtacgaatc cacatggtgg tttcgctgag
     1441 ctgacctgta gtaggatcaa caatagtgct accgtgtcgg tcatagagag aaggactaaa
     1501 catcgtgttc attgctatca ggttagcaat ataactgcca gcttcagggc gccatgtgtg
     1561 aatactacct tcacccaaat taagtgaaga tacaagctgt gaactagctc ggcgactact
     1621 catttgagta gactcttgat tgctcatctg ttttgattct tgattatcca tctgactagt
     1681 tagataccac ttattggtat tcagtccaga tacagtgccc tgtttcaggc gatagtcata
     1741 actaccagca acaatacggt ctttctgaat aaaagcattc ttatcagaag aatctgtcga
     1801 aataatttga acaccttcaa gagttttatc tccagagcct ccctcatttg tcactgcaac
     1861 ataggtaata ccagacgcat ggcctgcaat ttccagccgg tcagttttac tgttgtcatt
     1921 gccgagaacc gcgcccaaag ataatattcc gccctttcca tgccaattac ctttctgaac
     1981 ataggtctga ccgacatttg acgaactatt attgagaata acatgaccgt ttttctccag
     2041 agtcatgtta cctgtgacaa tgacggggtc aggcaaaaaa ggggcattaa tattattgat
     2101 tagcacagtc ccactattta ataaagtgtt aacagtttgg ttcatgcctg aaaaattcaa
     2161 ggtagcacct ttattaacaa taacaccagc ggtacgtgtc atagcttcaa ctgtccccat
     2221 ttttagaatg cctgcattga tgttggtgaa accagagtag gtattttttt ccgccaaaat
     2281 gagagttccc tcccctgatt ttgttaggga ttttccgtcc cagctattta tattatgatg
     2341 gctgagatta tctgccagaa tagtactaac agtaagttca gcgttttctt tcagattgaa
     2401 ctctccatga ctgtcgccat ctgtatcatt ccagctcaaa ccgtagatca atttattctg
     2461 gtcactgata tagctttttg tatagtcaat agcaccagta gtgagtaacg gattgatgat
     2521 agatatatta gaaacactga aacctgttat ccctttatct gcacttagca gtttatagtc
     2581 cttctgcgcc cagttattta taatagcttc cacttttaca ttgttgccaa atgacatatt
     2641 tccagaagct tccagactac tgattccagc taaatttaaa gaactgttct caccaaaaga
     2701 aacgtcacct gaaacagtga atttgttacc tgatagtgaa acagttgtac aatcaccagc
     2761 acataaatca ccttcaatag ttggtgtggt cccagtgtaa tcattgttga taattttcag
     2821 aatggatgaa ttgttaacct gtacatcacc agtaatgaca gaaccttcat ggagtattaa
     2881 tgaattgttt ccaccagtga tgtggatagc attacctgct acattttgtg ttccatcatc
     2941 ttctttacct gaaattatat atccgctatt agcaatggtc atattgctac catttatagc
     3001 atccccccaa tgtccaccat taccgcctaa aatagctcca ttgttgatca tagacaggtt
     3061 atctcctgtg atagcattac caccgtagcc atcataacca taaccgtaag cagcacctcc
     3121 gttgcctcca gcgaacgtgc ccttattgat tacagagagg ctgcttcctg tgatggcatc
     3181 acctccatta ccgccatcac cctcaccata attgttacct ccattaccac cggaaatagt
     3241 ccctttgttg attatgggta gattgactcc tgtgacggca tcacctccat tccccccatc
     3301 actatcacta taactatcgc caccatgtcc acctgaaata atttctccat ttattataaa
     3361 cagattgtct cctgtaacag cctcaccacc atcaccgtta tggtcagcac cgctaccgcc
     3421 gctaccacca agaatcatgc cttgattgat tatagacaag tcagatccgg taatagagtc
     3481 accaccatta ccgccacaac tattaccatc attattatca ccagcaccac catgacctgc
     3541 gctaatcata atatcagtgt taatccgtag cttagttcta gatgcatgag aggggttgga
     3601 agaagttata taccacgcat ccattccatc ttcaccagct cccaagggag aaagtccatc
     3661 aacaggtgtt aataattttt cataattgtc ctctgaaaaa tgaagttctt gagtacccga
     3721 aagaggaata gcaaaagcta ctggcccccc gagcaacagg gatgcaccaa caacaaaagg
     3781 agaaaaagac aatttgcatg ttttttcctt taccgtaggt aattctccgg cccccccctg
     3841 tgaacattgg gtcatattac aaaaaaattt gtgaatttga ttcatgcact atattatcag
     3901 taagtggttg ataaacccct aaaaagattc cgttttatcc ggaataaagg gacgatatat
     3961 gcaaaaacat attaaacaaa gcctcaacca aaacatgaga gctgtgttat atatcaatgc
     4021 aacgggagaa atttacctgt gtcttgcttt gaaaaaaaga gggggaaagc tatatgttcg
     4081 ataataaatt aataatttta ctatgatagt ctgcttcaga ccgcaacttt tcagttcttt
     4141 ctgtacgctt gcccaaagtt taaccacttt gtttttctat acacccaatg gttctatctg
     4201 ccccttgatg ttaatcccca aagccaataa catgaatgta ttcatatcac aactttccta
     4261 ccagactctc caatgcaata actgcataat tgtttttagt ggaaatgtat cgttttctta
     4321 aagagaagaa taacattcca ctttattatg ttcccaattt aattatatta atatatattg
     4381 gcattaatag gaaaatacat caggagaaat caaatgcaga catcaaacat aactaaccat
     4441 gaaagaaatg actcatcatg gatgtcaact gtcaaatcaa ctacagaagt aagttggaat
     4501 aaattgagtt tttgtgacgt tttactgaaa ataataactt tcggcatata ttctccacac
     4561 gaaactctag ccgaaaaata tagcgaaaaa aactaatgga tagtttctca ccatcactat
     4621 cacaagataa aatggacgga gagtttgccc acgcaaacat agatggcatt tcaatacggc
     4681 tatgccttaa caaaggcata tgttcggttt tttatcttga tggagataaa atacaatcca
     4741 ctcaactttc atcaaaagaa tataataact tgttaagttc actccccccc aaacaattca
     4801 acttaggaaa agtacatact attacagctc cggtatcagg gaattttaaa actcacaagc
     4861 ctgcaccaga agttattgaa acagccatta attgctgtac ctcgataata ccaaacgatg
     4921 attacttccc cgttaaagac actgatttta atagtgtttg gcatgatatt tacagagata
     4981 ttcgagcatc tgacagcaat agtacaaaaa tatatttcaa caatattgaa ataccactaa
     5041 aattaattgc agatctaatt aatgagttag gaattaatga attcattgat agcaaaaaag
     5101 agcttcaaat gctaagttac aaccaagtaa ataaaataat caattcaaac ttcccccaac
     5161 aagacctatg ttttcaaaca gaaaaacttt tgtttacttc actctttcaa gaccctgcat
     5221 ttatatctgc actaacatct gcattctggc aatctcttca catcacgtct tcctctgtgg
     5281 aacacatata tgcccaaatt atgtccgaaa acatagaaaa caggcttaat ttcatgcctg
     5341 aacaacgagt tattaacaat tgtggacata ttataaaaat caacgcagtt gtccctaaaa
     5401 acgacactgc aatctctgcc tctgggggga gggcttacga agttagctca tcaatattac
     5461 catcgcacat aacatgtaat ggtgttggta ttaataaaat agaaacaagc tatctcgttc
     5521 atgctggaac acttccaagc agtgaagggc ttcgcaatgc tatccctcca gaaagtcgtc
     5581 aagtatcatt tgccatcata tctcctgatg tttaagccaa tattatattg gctgccagac
     5641 tgtaaaaaaa cgttcttcag aacagctcta taaactatta aaccaaagct aaaaggatgg
     5701 ggaccaatag aatctcagcc catccttttt atatcattat gaaccgcccc ggaaatcctg
     5761 gagactaaac t
//
LOCUS       DADLSA010000201         5712 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.202, whole genome shotgun sequence.
ACCESSION   DADLSA010000201 DADLSA010000000
VERSION     DADLSA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.202"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..1537)
                     /locus_tag="JN691_003822"
     CDS             complement(<1..1537)
                     /locus_tag="JN691_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAY7245115.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPMQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHFSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ECWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQLYMLDVGQ
                     GLAM"
     gene            complement(1744..2028)
                     /gene="ihfB"
                     /locus_tag="JN691_003823"
     CDS             complement(1744..2028)
                     /gene="ihfB"
                     /locus_tag="JN691_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAY7245116.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(2188..3861)
                     /gene="rpsA"
                     /locus_tag="JN691_003824"
     CDS             complement(2188..3861)
                     /gene="rpsA"
                     /locus_tag="JN691_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAY7245117.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(3972..4655)
                     /gene="cmk"
                     /locus_tag="JN691_003825"
     CDS             complement(3972..4655)
                     /gene="cmk"
                     /locus_tag="JN691_003825"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAY7245118.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(4828..5592)
                     /locus_tag="JN691_003826"
     CDS             complement(4828..5592)
                     /locus_tag="JN691_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7245119.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         1447 a         1508 c         1346 g         1411 t
ORIGIN      
        1 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
       61 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
      121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
      181 aacattcagc aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
      241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
      301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
      361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
      421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
      481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
      541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
      601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
      661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
      721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
      781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
      841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
      901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
      961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
     1021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
     1081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
     1141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagatattcg ccatacagca
     1201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
     1261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
     1321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
     1381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
     1441 tcagaaacgc aagggttaat gtcccaggca attggggcaa aatcagcaac ggaaaaattc
     1501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
     1561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
     1621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
     1681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
     1741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
     1801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
     1861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
     1921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
     1981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
     2041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
     2101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
     2161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
     2221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
     2281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
     2341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
     2401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
     2461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
     2521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
     2581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
     2641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
     2701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
     2761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
     2821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
     2881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
     2941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
     3001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
     3061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
     3121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
     3181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
     3241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
     3301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
     3361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
     3421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
     3481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
     3541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
     3601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
     3661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
     3721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
     3781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
     3841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
     3901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
     3961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
     4021 acttgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
     4081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
     4141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
     4201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
     4261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
     4321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
     4381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
     4441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
     4501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
     4561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
     4621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
     4681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
     4741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
     4801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
     4861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
     4921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
     4981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
     5041 aattgcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
     5101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
     5161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
     5221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
     5281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
     5341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
     5401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
     5461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
     5521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
     5581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
     5641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
     5701 ccgacaatgt gc
//
LOCUS       DADLSA010000202         5697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.203, whole genome shotgun sequence.
ACCESSION   DADLSA010000202 DADLSA010000000
VERSION     DADLSA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.203"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            67..282
                     /gene="xisR"
                     /locus_tag="JN691_003827"
     CDS             67..282
                     /gene="xisR"
                     /locus_tag="JN691_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HAY7245120.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            284..1519
                     /locus_tag="JN691_003828"
     CDS             284..1519
                     /locus_tag="JN691_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7245121.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVS
                     SLGARWNAAVKRAGIRRHNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQIAMLNARLS"
     gene            2006..2896
                     /gene="sitA"
                     /locus_tag="JN691_003829"
     CDS             2006..2896
                     /gene="sitA"
                     /locus_tag="JN691_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAY7245122.1"
                     /translation="MLLGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDNPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
     gene            2896..3723
                     /gene="sitB"
                     /locus_tag="JN691_003830"
     CDS             2896..3723
                     /gene="sitB"
                     /locus_tag="JN691_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAY7245123.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            3720..4577
                     /gene="sitC"
                     /locus_tag="JN691_003831"
     CDS             3720..4577
                     /gene="sitC"
                     /locus_tag="JN691_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAY7245124.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            4574..5431
                     /gene="sitD"
                     /locus_tag="JN691_003832"
     CDS             4574..5431
                     /gene="sitD"
                     /locus_tag="JN691_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAY7245125.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
BASE COUNT         1517 a         1305 c         1373 g         1502 t
ORIGIN      
        1 cctcattaat aatcaacatc gggcgtcaat ttcagcctaa cattggcgcc tgccagaggt
       61 gatgcgatgg cacaagtaat ctttaatgaa gagtggatgg ttgaatacgg cctgatgctt
      121 cgcactggtc tgggtgccag acaaattgaa gcataccgcc agaactgttg ggtggaaggc
      181 ttccacttca aacgagtatc tcctttaggg aagccagaca gtaagcgagg gattatctgg
      241 tacaactatc cgaagataaa tcagtttatc aaagactcat gatatgtcta aattaccaac
      301 aggtgtcgag attcgaggta aatacattcg catctggttc atgtttcgag gaaaacgatg
      361 tcgggaaaca ttgaaaggct gggagattac taacagtaac attaaaaagg ccgggaattt
      421 aagagcgttg atagttcatg aaatcagttc cggtgaattt gagtatttaa gacgttttcc
      481 ccagtccagc actggggcta aaatggtgac aacgagggtc ataaaaacat tcggggagct
      541 ttgtgatatc tggacaaaaa ttaaagagac agagttaaca acaaacacaa tgaagaaaac
      601 gaaatcacaa ttaaaaacac tcaggataat aatttgtgag agtaccccga tatcgcatat
      661 tcgttatagc gatatcttaa actaccggaa tgaactgctg catggagaaa cgctttacct
      721 agataatcca agatccaaca aaaaaggaag aaccgtgcgc acagttgata actatatcgc
      781 cctgctctgt tcgttgttac gttttgcgta tcagtcggga tttatatcaa ccaaaccatt
      841 tgaaggagta aaaaaattac agcgaaacag aataaagcct gacccgttat ctaaaacaga
      901 attcaatgca ttaatggaaa gtgaaaaagg acagagccaa aacttgtgga aatttgccgt
      961 ttactccggg cttcgtcacg gggaactggc agctctggcg tgggaggatg tggatttcga
     1021 gaagggaatt gtgaatgtca gaagaaacct gacgatactt gatatgttcg gtcccccaaa
     1081 aacaaatgcc gggatccgga cggtaacatt actgcagccg gctcttgaag cactgaagga
     1141 gcaatacaaa ctgaccgggc atcatcgcaa aagcgaaatc actttttatc atcgggagta
     1201 cggcagaact gaaaagcaaa aactgcattt tgttttcatg cccagggtgt gtaacgaaaa
     1261 acagaaacct tattactcgg taagcagttt gggtgcaaga tggaatgcag cagtaaaacg
     1321 tgctggtatt cgccgccata atccgtacca tacgcgacat acttttgcct gctggctgtt
     1381 gacggcagga gcgaacccgg catttatagc cagccagatg gggcatgaaa ctgcgcaaat
     1441 ggtgtatgaa atttacggta tgtggattga tgacatgaac gacgaacaga tagccatgtt
     1501 gaatgcgcgg ttatcgtagt tgcaaagttt gcccccaatt tgccccattt agtaccagag
     1561 aactgaaata atgcaagaaa atcaacaaat tacaaagaaa gaacaataca acctgaacaa
     1621 gtaagggcaa aaatcacaac tatctgactc ataagtattt tatttatttt tcaatatgtt
     1681 aaacattatc agcgacaaca ataagctacg ataacccact ctttttttgc cccttttttg
     1741 ccccttctac agcgttttgc cccatttttg ccatcaacaa ttttcctcat aaagtcattt
     1801 caaagcctaa tagctgctct acttttttcc tcaatatcat gtcgccagca ggaaacaacc
     1861 taatccctgt tttaggcctc aaagctgtta atgataatta ttttcatttt gtataggtta
     1921 tgctataaaa catagccgtt gctatgtttg ttctaatttt gctcactata ggtactaaat
     1981 tatgcactca ataaaaaaag taaccatgct cttggggtgc ctcgccctca cctgctcgat
     2041 cgcatttcag gcaagtgcaa ctgaaaaatt caaggtcatt acaacattta ccatcatcgc
     2101 ggatatggcc aaaaacgtgg ctggagatgc tgcagaagtc tcatccataa ccaagcctgg
     2161 tgcagaaatt catgagtatc agcctacccc tggcgatatt aaacgtgcgc agggggcaca
     2221 actgattctc gccaatggta tgaatctgga attgtggttc caacgctttt accagcatct
     2281 caatggggtt ccagaagtaa ttgtctcttc gggtgtgacg ccagtaggga tcaccgaagg
     2341 accctatgag ggcaaaccta atccccatgc atggatgtcg ccagataatg ctctgattta
     2401 cgtcgataat attcgtgatg cgttgataaa atacgatccg gcaaatgcac aaacctacca
     2461 acgtaatgcc gatacttata aagccaagat tacccaaacc cttgcccccc tgcgtaagca
     2521 gattacggaa ctccctgaga atcagcgatg gatggtcacc agtgaagggg ctttttctta
     2581 tctcgcacgc gatttggggc taaaagagct ttatctgtgg ccgattaatg ccgatcaaca
     2641 aggaacaccg cagcaggtac gtaaggttgt tgatatagtt aagaaaaatc atataccggc
     2701 agtctttagc gagagtacga tttccgataa tccagcgcgt caggttgcgc gtgaaaccgg
     2761 cgcacactac ggcggtgtgc tctatgtcga ttccctgagc acagaaaacg gcccggtacc
     2821 tacgtatatc gaccttctta aggttactac cagtacgctg gtacagggaa ttaaagccgg
     2881 gaaaagggag aaataatgat gcaatctgca ggcattgtcg ttaatgatgt tactgtcacc
     2941 tggcgtaacg ggcacacagc actgcgcgat gcatccttca cagtaccaag tggatctatt
     3001 gccgctctgg ttggggtaaa cggttccgga aaatcgacgt tgtttaaagc gattatggga
     3061 tttgtgcgtc tgaccagcgg aaagatatct gttctgggta ttcccacgcg acaggcgcta
     3121 cagaaaaacc tggttgccta cgtccctcag tcagaagagg ttgactggtc atttcctgta
     3181 ctggtggaag atgtggtgat gatgggccgc tatgggcata tgggtatgtt gcgtatagcc
     3241 aaaaagaggg atcgtcagat tgtcaccgat gcgctggaac gtgtcgatat ggtggatttt
     3301 cgccatcgtc aaatcggcga gctttccggt ggacaaaaga aacgcgtctt tctggcgaga
     3361 gcgattgcac agcagggtga tgtgatcctg ctggatgaac cttttaccgg cgttgatgtg
     3421 aaaaccgaag cgaaaatcat cagcctgttg agggagttac gcgcggaagg caaaacgatg
     3481 cttgtctcaa cgcacaatct cggctcagtc acgacatttt gcgattatac ggtcatggtc
     3541 aaaggcaccg tcctggcaag cgggccgaca gacaccactt ttacagccga gaacctggag
     3601 ctggctttta gcggcgtact gcgccatgtc acacttaacg gctcggaaga aagcattatt
     3661 actgacgatg aacgcccttt tgtggcacat cgaccgtcag cggtgcagag ggaagcaaga
     3721 tgaacgtgct actggaaccc ttcagctatg aatatatgct caatgcgatg tgggtctcgg
     3781 caatggtcgg cggtctctgc gctttcctgt catgctatct gatgctaaaa ggctggtcgc
     3841 tgattggcga tgcgctgtcg cactctattg tcccgggtgt tgcgggggca tatatgctgg
     3901 ggcttccgtt ttctctggga gccttctttt ccggcggact ggcagcgggc agcatgctgt
     3961 ttcttaacca acgcacccgc ttaaaagaag atgccatcat cggcctgatc ttctcgtcct
     4021 tttttggcct ggggctgttt atggtgtcat tgaacccgac atccgtgaac attcagacca
     4081 tcgtcctggg caatattctg gcaatcgatc cggcggatat cctgcaactc actatcattg
     4141 gcattctctc gatcatcgtg ctttttttca aatggaaaga tcttatggtg acctttttcg
     4201 acgagaatca cgcccgtgct attggattac atccgggcag attaaaaatc ctcttcttca
     4261 ccctgctgtc ggtttcgacc gttgcggcgc tgcaaacggt tggtgccttt ctggtgatct
     4321 gtctggtggt gactcccggt gcaacagcat ggttgttaac cgatcgtttt ccccgcctgc
     4381 tcataattgc tgtaactatt ggcagtgtaa ccagtttcct cggcgcgtgg gtcagttact
     4441 ttctggacgg cgcgacaggt ggaatcatcg tggtggcgca gacactgctg tttcttttag
     4501 catttgtatt tgcgcccacc catggacttt tggcaaatcg tcggcgtgcg cataaagcgc
     4561 tggaggatcg ctcatgatgg cattattact ggagccactg cagttcacct ttatgagtca
     4621 cgcactgctg atttcactgg tagtttccat accctgtgcg ctgctgtcgg tctttctggt
     4681 cctgaaaggt tgggcactga tgggtgacgc gatgagtcat gcggtttttc ctggcatcgt
     4741 gcttacctgg attctggggt tacctctggc tacaggggca ttcgtcgccg gagtattctg
     4801 cgcggtggcg acgggatacc tgaaagacaa cagccgaatc aagcaagata cagttatggg
     4861 gattgttttt tctggcatgt ttgccgcagg tctgatcttg tatattgcaa ttaaaccaga
     4921 cgtacatctt gaccatattc ttttcggcga catgctcggg ataaccatcg gcgatataat
     4981 ccagacggtg attattgccg ggctggttac acttgttatt agcgtaaaat ggcgggattt
     5041 tttgctgttc agctttgatt atcaacaggc gcaggtaagc ggtttgcata cgaggtggct
     5101 gcattatggg ctgctgtgca tggtttccct gaccattgtg gcgacgctga aagcagtagg
     5161 tatcattctc tctatttctc tgctcattgc gcctggcgcc attgcagtac tcctgacaca
     5221 acgctttcat attgcgctcc tactggcgac tggcatatcg gtaatagtgt caatgactgg
     5281 tgtctggctg tcctttttta tcgacagcgc accagctccg acgattgtcg tcttattcgc
     5341 ggtcgtgttc atcatgacgt ttaccgttac cagcatcaac gcacgcacaa agggaaacgc
     5401 ccatacacag gatctgttat cacccaatta gcacaacatc cgaggacagc atcgtccgcc
     5461 agcctacaga agtccggaaa gccatggaag gtcgttggga ggaggtacga attctatcat
     5521 gcaaaaaata cgtaaaatcg ataacgccta gaaatcattc aatactcgca ctatcgaaag
     5581 tttaccagcc aaccgcggca cgttcctgca tacgacgtgc cgcagcttct gttattccac
     5641 gataaaattc ctaaccgaat tcaaatcctt cagactattc agctcttcct tcagctc
//
LOCUS       DADLSA010000203         5686 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.204, whole genome shotgun sequence.
ACCESSION   DADLSA010000203 DADLSA010000000
VERSION     DADLSA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.204"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            64..972
                     /locus_tag="JN691_003833"
     CDS             64..972
                     /locus_tag="JN691_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAY7245126.1"
                     /translation="MKAPVSLTSFHPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1018..3009)
                     /gene="tkt"
                     /locus_tag="JN691_003834"
     CDS             complement(1018..3009)
                     /gene="tkt"
                     /locus_tag="JN691_003834"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY7245127.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3287..4045
                     /locus_tag="JN691_003835"
     CDS             3287..4045
                     /locus_tag="JN691_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7245128.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVAPGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4251..5171)
                     /gene="speB"
                     /locus_tag="JN691_003836"
     CDS             complement(4251..5171)
                     /gene="speB"
                     /locus_tag="JN691_003836"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAY7245129.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5307..>5686)
                     /locus_tag="JN691_003837"
     CDS             complement(5307..>5686)
                     /locus_tag="JN691_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245130.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYIVN
                     AGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1364 a         1521 c         1512 g         1289 t
ORIGIN      
        1 tgtcggctgt tttctcctca tctcatgtga catattccca tactttactc aaggaagatt
       61 gtcatgaaag cacctgtttc attaacctca tttcacccac aaaaatctct tgctatcgcc
      121 attggcgtgc ttgccgtggt tgtgctgcca tttctctcgt attacaccgt taatgaaggt
      181 gaacgcggca ttttgctgcg ctacggaaaa atcgtaaaag ttgccgagcc ggggctgggt
      241 tttaagatcc cttttatgga aagcgtggag aaaatttcca cgcgtaatca ggctgtggtc
      301 tatcagggat tacaggccta cagccgcgac cagcaaccag cacaaatgac agtatcagtc
      361 agcttccaca ttaaaccttc agaagcaggc gcggtttata ccacctacaa cactattgaa
      421 gcattaaaag atcgtctaat tgtccgtcag ttgccaactc agctggagaa cattttcggc
      481 caatataccg ccatcagcgc cgtacaggat cgtacaaaat tggttcagga tctgcaaaat
      541 gccatgcgca aagccgttgt tgggccagtg gtaattgacg gggtccagat tgaaaatatt
      601 gatttttctg acgcttatga aaagtcgatt gaagaccgaa tgaaagcaga agtcgccatt
      661 gccacgcgta agcaaaatct ggaaacagag aaaatccagg cgcaaatagc ggtaactcag
      721 gcccaggcag aagcagacag taaactggcg gcagccaaag cagaagcgga aactattcgc
      781 gtaagaggcg cggcagaggc tgaaactatt cgtttaaaaa gtgcggctga agcagaagca
      841 atccgcctgc gcggtgaagc tctgcgggat aatcccggac ttgtagccct caccactgcc
      901 gaacgttggg acggtaaact gcccgatacc atgatccccg gcagcacagt tccgtttatc
      961 tctactaagt aaaaaaaggg ccgctttcgc ggcccttctc acaaaacgtc ttactgatta
     1021 cagcagcgct tttgctttcg caacaacgtt atcaacagtg aagccgaact cttcaaacag
     1081 cagctctgcc ggagcagatt caccgaaggt ggtcatgccg acgatagcgc cgttcaggcc
     1141 aacatacttg taccagtagt cagcaatacc cgcttctacc gcaacgcgtg cagtaaccgc
     1201 tttcggcagt acggattcac ggtaagccgc atcctgcttg tcaaatgcgt cggtagacgg
     1261 catggacacc acgcgcgctt tcacgccttc ggcagtcagt ttttcgtagg cagcaaccgc
     1321 cagttcaact tcagaaccgg tggcgatgaa gatcagttcc ggctgaccag cacagtcttt
     1381 cagcacataa ccaccgcgcg cgatgtttgc cagctgctct tcagtacgtt cctgctgcgc
     1441 caggttctga cgggaaagga taagcgcagt cgggccgtcc tgacgctcaa cgccgtattt
     1501 ccacgcgacc gcggattcaa cctggtcaca cggacgccat gtagacatgt tcggggtcac
     1561 gcgcagagaa gcgacctgct ctaccggctg gtgagtcggg ccatcttcgc ccagaccgat
     1621 ggagtcgtgg gtgtaaacca tcacctggcg ctgtttcatc agcgcagcca tacgtacggc
     1681 gttacgtgcg tattccacga acatcaggaa ggtggaggtg tacggcagga agccaccgtg
     1741 cagggagata ccgttagcaa tcgcggtcat accgaactcg cgaacaccgt agtggatgta
     1801 gttacctgca gcatcttcgt tgattgcttt agaaccagac cacagggtca ggttagacgg
     1861 tgccaggtca gcagagccgc cgaggaattc aggcaacagc gggccgaacg cttcgatagc
     1921 attctgcgac gctttacggc tggcgatttt cgccggatta gcctgcagtt tagcgataaa
     1981 ctctttcgct ttggcgtcga agtcagacgg catttcgcct ttcatacggc gggtaaattc
     2041 agccgcttcc tgcggataag ctttcgcgta agccgcaaac ttctcattcc atgcagattc
     2101 tttcgcctgg cctgcttctt tcgcatccca ctgagcatag atttcagacg ggatttcgaa
     2161 cggtgcatat ttccagccca gctgttcgcg ggtcagggca atttcagcgt cgcccagcgg
     2221 cgcaccgtgg gagtcgtgag taccggcttt gttcggggaa ccgaaaccga tgatggtttt
     2281 gcacatcagc agggacggtt tgtcagtcac tgcgcgcgct tcttcaactg cgcgtttgat
     2341 ggatgccgcg tcatgaccgt cgatgtcgcg aataacgtgc cagccgtaag cttcgaaacg
     2401 cattgctgtg tcgtcggtga accagccttc aacgtgacca tcgatagaaa taccgttgtc
     2461 atcgtagaac gcaatcagtt tacccagctt cagcgtaccc gccagagagc aaacttcgtg
     2521 ggagatgcct tccatcatgc agccgtcgcc catgaaggcg taggtgtagt ggtcgacaat
     2581 gtcgtggccc ggacggttaa actgcgccgc cagcgttttt tctgcaatcg ccataccgac
     2641 tgcgttggca ataccctgac ccagcggacc ggtggtggtt tccacaccag cggtataacc
     2701 tacttccggg tggcccggag ttttagagtg cagctgacgg aagtttttca gttcttccat
     2761 cggcagatcg taaccggtga ggtgcagcag gctgtagatc agcatggagc cgtggccgtt
     2821 ggacagcacg aagcggtcac ggtcagccca ggacggattc tgcgggttgt gtttcaggaa
     2881 atcacgccac aggacttcgg caatgtcagc catacccata ggggcacccg ggtgaccgga
     2941 tttggctttc tgtactgcgt ccatgctcag cgcacgaata gcattggcaa gctctttacg
     3001 tgaggacatt ttgactccag atcggatgat gaagggcacg cccttaacga cttgacgaca
     3061 gcgcgttttg ggctacgccg gaaaatttgc caacaattta ccgcaagccg cgcgtcatgt
     3121 acatggaaca tccttttgcc gcttcagaaa tctctggatc atgctcgcat gttgcgcaat
     3181 ctgctcgccc gtccgctgcg cttttcctta tactgagact gagcgtcgat tcacctgcaa
     3241 acggcgcatt tttagaataa tcctgacctt gtgcggaaga gaaaacatga aaattcgcgc
     3301 cttattggta gcaatgagcg tggcaacggt actgaccggt tgccagaata tggactccaa
     3361 cggactgctc tcatcaggag cggaagcttt tcaggcttac agtttgagtg atgcgcaggt
     3421 gaaaaccctg agcgatcagg catgtcagga gatggacagc aaggcgacga ttgcgccagc
     3481 caatagcgaa tacgctaaac gtctgacaac tattgccaat gcgctaggca acaatatcaa
     3541 cggtcagccg gtgaattaca aagtgtatat ggcgaaggat gtgaacgcct ttgcaatggc
     3601 taacggctgt atccgcgtct atagcgggct gatggatatg atgacagata acgaagtcga
     3661 agcggtgatc ggtcacgaga tggggcacgt ggcgttaggc catgtgaaga aagggatgca
     3721 ggtggcgcct ggtacaaatg ccgtgcgagt agctgcggcc tctgcgggcg ggattgtcgg
     3781 cagtttatct caatcacaac ttggtgatct gggcgagaaa ttagtcaatt cgcaattctc
     3841 ccagcgccag gaagcagaag ccgatgatta ttcttacgat cttctgcgcc aacgcggcat
     3901 cagcccggca ggtcttgcca ccagctttga aaaactggcg aagctggaag aaggccgtca
     3961 aagctcaatg tttgacgacc atcctgcatc cgccgaacgt gcccagcata ttcgcgatcg
     4021 catcagcgcg gatgggatta agtaaagcct ggtggtgtcg aacgtaggtc agataaggcg
     4081 ttcacgccgc atctgacatg aatggcacat ttgttacctt gtacgcattg ccggatgcga
     4141 tgctggcgca tcttatccgg cctacgggtg ccgaacgtag gtcggataag gcgttcacgc
     4201 cgcatccgac attaatggca cgttttaccc gtgcgcatcg catctggtgc ttactcgccc
     4261 tttttcgccg cctgaatata cagcatctcc agcgccagcg tcgccgcagc cagagcggtg
     4321 atttccgact gatcgtatgc cggagccact tccactacgt ccatcccaac gatgttgaga
     4381 tctttcaggc cgcgtaccag tttaatggcg cgatcggagg tcaggccgcc aatcactggc
     4441 gtaccggttc ccggcgcgaa agcaggatcc aggcagtcga tatcaaaagt caggtaaacc
     4501 ggcatatcac ccacaatctg tttcacctgg gcgataacgt catccacgct gcgatcgttc
     4561 acctggcagg cgtccagcac ggtaaagccg ttgtctttat caaactcggt acgaatacca
     4621 atctgcacgg aatgattcgg gtcgatcaga ccttctttcg gcgcggtata gaacatagtg
     4681 ccgtggtcaa attcacaacc gttcgcatag gtatcggtgt gggcgtcaaa gtgtaccagc
     4741 gccattttgc cgaaatgctt cgcatgagca cgcagcagcg gcagcgtaac aaagtggtca
     4801 ccaccgaagg agagcatacg cttaccggca gccagcagct tctcggcgtg cgcctgcagc
     4861 ttttcgctca tctcacgggc atcgccaaag gcatatacca gatcgccgca gtccacgacg
     4921 ttcagacgct cacgcatgtc gaaattccac gggaagcggt tgtgttccca ggccagattc
     4981 gtcgaaacct gacggatcgc tgccggaccg tggcgaccac ccgcacgacc agaagtggcc
     5041 atatcgaacg gcacgccagt aatcacccag tctgcatcgc tgtcatacgg ctggaagttc
     5101 atcggcaggc gtaaaaaacc aaaggcattg gaaaccagtg agttatcgta ttgatgacct
     5161 aaggtgctca tggatggact cctattacaa agtcgatata aaaaaaccct tccgcgtcgt
     5221 taggcccgac gaggaagggt tggatttgcg aaacacaagt ttgtgctaat tatcgcctta
     5281 aatgggcgag gcatcaagcg aggttgctat ttccgacacg gtggcggcgc atagccattg
     5341 ttatctgccg gaaaggcttg tccagagcac ggcggcagcg cgttttgcgc cgggccttgt
     5401 aattcaaagg cggcaaagcc gattaccccg gtattacgca catcgccttg cgccgaattg
     5461 gctgcgtagg aatcagcaac gttggcgaac tgaaaagccg cttcagtatg tttatctttg
     5521 cggaatcctt taatcgctaa cgaatctccg gcattcacaa tatagccgtt gttattcagc
     5581 gaaccttgct tgccgttcag cacatccagg ccatcaaccg tagcaacgat ctcgtagttg
     5641 gtgttacgac tgtaattacg gacatataat tgataattca ttccga
//
LOCUS       DADLSA010000204         5675 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.205, whole genome shotgun sequence.
ACCESSION   DADLSA010000204 DADLSA010000000
VERSION     DADLSA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.205"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..785
                     /gene="msrP"
                     /locus_tag="JN691_003838"
     CDS             <1..785
                     /gene="msrP"
                     /locus_tag="JN691_003838"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HAY7245131.1"
                     /translation="NLPLTPADKVSGYNNFYEFGLDKDDPAANAGSLKTDPWTLKISG
                     EVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFPLHKLLALAEPTSNAKY
                     VAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLTLMTVGVYGKALPPQNG
                     APVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYGFYANVNPHVDHPRWSQ
                     ATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF"
     gene            786..1421
                     /gene="msrQ"
                     /locus_tag="JN691_003839"
     CDS             786..1421
                     /gene="msrQ"
                     /locus_tag="JN691_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HAY7245132.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            1679..2329
                     /gene="zinT"
                     /locus_tag="JN691_003840"
     CDS             1679..2329
                     /gene="zinT"
                     /locus_tag="JN691_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAY7245133.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2999..3088)
                     /locus_tag="JN691_003841"
     tRNA            complement(2999..3088)
                     /locus_tag="JN691_003841"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3052..3054),aa:Ser,seq:cga)
     gene            3182..3979
                     /gene="mtfA"
                     /locus_tag="JN691_003842"
     CDS             3182..3979
                     /gene="mtfA"
                     /locus_tag="JN691_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAY7245134.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEIAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            4080..4155
                     /locus_tag="JN691_003843"
     tRNA            4080..4155
                     /locus_tag="JN691_003843"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4113..4115,aa:Asn,seq:gtt)
     gene            4317..5579
                     /locus_tag="JN691_003844"
     CDS             4317..5579
                     /locus_tag="JN691_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7245135.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT         1504 a         1328 c         1311 g         1532 t
ORIGIN      
        1 ataacctgcc actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg
       61 ggctggataa agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac
      121 tgaaaatcag cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc
      181 gcttcccgct ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg
      241 tgccgtggat tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg
      301 cgaagtatgt cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc
      361 gctttatcgg cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa
      421 tgcatccgct cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg
      481 gcgcgccggt gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg
      541 tcagtattaa gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg
      601 aatacggttt ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta
      661 ccgaacgatt tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt
      721 ttaatggtta cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt
      781 tctaaatgcg tctgacagca aaacaggtga tatggctgaa agtttgcctg catcttgccg
      841 gattgttgcc gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc
      901 cggtgaaaga tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct
      961 tgttaatcac acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc
     1021 tgttaggctt atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg
     1081 agttgggcgt gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa
     1141 cgttaggaat tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga
     1201 tgcagcgaaa actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga
     1261 tcctggcccc aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct
     1321 acgctgggct ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca
     1381 accggttacg caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac
     1441 ttcgcgttat cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc
     1501 aagttatgtt tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata
     1561 agatagttca atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt
     1621 ttcatatgtt acaatataac attacacata atgtacatta actctggagg aaattgtttt
     1681 ggcgattcgt cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc
     1741 cttttcgcat ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc
     1801 taatggtgtt tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt
     1861 ctggcaatcc gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa
     1921 agcggatgca gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta
     1981 tccgacagat atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa
     2041 tgaaacaaca tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg
     2101 caagaaaggc gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata
     2161 tatacaattt agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat
     2221 gggtaatgat tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc
     2281 ctatcagttg agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc
     2341 tgacttcagt tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa
     2401 ataagtaaga caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt
     2461 agaaaaaaac tgagaaaaag gcgaacaata aatacacaac tcagccatac agactggcag
     2521 cgcaaatgtt tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta
     2581 attttggtgt ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg
     2641 ggaaattcaa caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg
     2701 cgttcctttt ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca
     2761 actttgcggc agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac
     2821 gactgaataa ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc
     2881 gctgaatgtc acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta
     2941 cgattgttct gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaagtg
     3001 gcggagagag ggggatttga acccccggta gagttgcccc tactccggtt ttcgagactg
     3061 gtccgttcag ccgctccggc atctctccgt tcagatggtt gccatgatgc caggaaattt
     3121 ggcattttaa cagtccctgt ccgtgcaatt ttgttcaagt gacgagtttg cgagcaaaac
     3181 gatgattaag tggccctgga aagtacaaga atcagcacat caaactgccc ttccctggca
     3241 ggaagcacta tcgatccccc ttttaacgtg tctgacagaa caggaacaaa gcaaattggt
     3301 cgctcttgcc gaacgttttt tacagcaaaa acggcttgtt cctttacagg gctttgagct
     3361 gaattcatta agaagctgcc ggatagcact tctattttgc ctgcccgttc tggagttagg
     3421 actggaatgg ctggatggtt ttcatgaagt cttaatttat cctgcgccat ttgtggtcga
     3481 tgatgaatgg gaagacgata tcggtctggt gcataaccaa cgtattgttc agtcaggtca
     3541 gagctggcag caagggccta tcgttttgaa ctggttggat atacaagatt cttttgatgc
     3601 ttctggtttt aacctgatta ttcatgaaat cgctcataag ctggacaccc gtaacggcga
     3661 tcgcgccagc ggagttccct ttatttcgtt gcgtgaggtt gctggctggg aacacgatct
     3721 tcatgctgca atgaacaaca ttcaggaaga aatcgaatta gttggtgaga atgcggcgag
     3781 cattgatgct tatgctgcca gtgatcctgc tgaatgtttt gccgtacttt ctgaatattt
     3841 ctttagcgcc ccagaacttt ttgctcctcg tttcccttca ttgtggcaac gtttctgtca
     3901 attttatcaa caagatcctt tgcagagact gcatcacgct aatgatacag actcgttttc
     3961 ggcgacgaat gttcattaat taacaacttt gcagattaat taaccaattg aaatggctta
     4021 tgaaatttag tgttgacaga caaggtaccg ctaagtaata tgcgccccgt tctcacgatt
     4081 cctctgtagt tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc
     4141 cagtcagagg agccaatttt ctgttttcat gcatccttgc gaatctttat ctgatgttga
     4201 ttcaacaggt tagtgtgaaa actcttctcg gtgcattttg attttaccct acgcatcggg
     4261 aaaaattggt ggtcaaatct ggggtcaggt tagttcgata atggagtgac ccccatatat
     4321 cccttaccga cgcaaaaatc cgcaccctca agccttctga taaacccttt aaagtctccg
     4381 attctcacgg tctgtatctg ctggtcaagc cgggtggctc ccgccactgg tatctcaaat
     4441 accgtattag cggtaaagaa tcccgcattg cgctgggtgc ctatccagcc atctccctgt
     4501 ctgatgcgcg acagcaacgt gaaggtatcc gtaaaatgct ggcgctgaat atcaacccgg
     4561 tacagcagcg ggctgctgaa cgtggctcac gaacaccgga gaaagttttt aaaaacgtgg
     4621 cgctggcgtg gcataaaagt aacaggaaat ggtcgcagaa caccgccgac cgtctgcttg
     4681 ccagcctgaa caatcacatc tttccggtca tcgggaacct acctgtatca gaacttaaac
     4741 cccgtcattt cattgacctg ctgaaaggga tcgaggaaaa aggtctgctg gaggttgcgt
     4801 cccgcacacg gcagcacctg agtaacataa tgcgccatgc ggtccatcag gagttaatcg
     4861 atacgaaccc tgcagcaaac cttggcggcg tgaccacacc tcctgtcaga cggcactatc
     4921 ctgccctgcc gctggagcgg ctgcctgaac tgcttgaacg tattggggca tatcatcagg
     4981 gccgtgaact gacccggcat gccgttctgc tgatgctgca tgtgttcatt cgctccagtg
     5041 aactgcgttt cgcccgctgg tcagagattg atttcacaaa ccgagtctgg acgatacccg
     5101 cgacgcgaga acccattatt ggcgtgcgtt attccggccg cggggcaaaa atgcgaatgc
     5161 cgcatatcgt ccccctctca gaacagtcca tcgccattct gaaacagatt aaggatatca
     5221 ccggtaataa tgaactgatc ttccccggcg accataaccc gtataagcca atgtgtgaaa
     5281 acacggtcaa taaggcactg cgggtgatgg gttacgacac gaaaaaggat atctgcggtc
     5341 acggcttccg ggcaatggca tgcagtgcgc tgatggaatc gggtttatgg gcaaaggacg
     5401 cagtagaacg ccagatgagt catcaggagc gcaataccgt gcgcatggct tatattcata
     5461 aggcagagca cctggaagcc cgcaaagcga tgatgcagtg gtggtcggat tatctggaag
     5521 catgccgaga atcttatgca ccgccttata caattggtaa aaataagttt atcccatagt
     5581 atactaacga atcccacact ctggcaaatt accaccacct ctacaggagg tggttctata
     5641 tagactttta tcaacttcat gacgaaaaac accta
//
LOCUS       DADLSA010000205         5672 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.206, whole genome shotgun sequence.
ACCESSION   DADLSA010000205 DADLSA010000000
VERSION     DADLSA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.206"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..979
                     /locus_tag="JN691_003845"
     CDS             <1..979
                     /locus_tag="JN691_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="involved in the transport of nitrate and nitrite;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HAY7245136.1"
                     /translation="CGFAGANFASSMGNISFFFPKAKQGSALGINGGLGNLGVSVMQL
                     VAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSR
                     ASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPF
                     IGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGL
                     FLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQREAVTETAAALGFISAIGAV
                     GGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK"
     gene            1061..4801
                     /locus_tag="JN691_003846"
     CDS             1061..4801
                     /locus_tag="JN691_003846"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY7245137.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFQEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            4798..>5672
                     /gene="narH"
                     /locus_tag="JN691_003847"
     CDS             4798..>5672
                     /gene="narH"
                     /locus_tag="JN691_003847"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY7245138.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSSPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEAASTEREVDL"
BASE COUNT         1327 a         1530 c         1549 g         1266 t
ORIGIN      
        1 atgcggtttt gcaggtgcaa actttgcttc gagcatgggc aatatcagtt tcttctttcc
       61 aaaagccaaa caagggagtg ctcttgggat taatggcgga ttaggaaact taggtgtaag
      121 tgtaatgcag ctggttgcac cgctggtcat ttttgtacct gtatttgcct ttctcggcgt
      181 caatggcgta ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg catggatttg
      241 ggtaccgcta ctggcgattg ccacgatcgc cgcctggtca gggatgaatg atatcgccag
      301 ttcacgcgcc tcaattgccg accagctccc tgtcttacaa cgcctgcatc tctggctgct
      361 gagcctgctt taccttgcca ccttcggttc gtttatcggt ttttctgcgg gttttgccat
      421 gctggcaaaa acccagttcc cggatgtgaa tattctgcgc ctggcgttct ttggcccatt
      481 tattggtgcc atcgcgcggt cggttggtgg tgctatttcc gataaattcg gcggcgtgcg
      541 ggtgacgttg atcaacttta tttttatggc gattttcagt gccctgctgt tccttacctt
      601 accgggcaca ggctccggta atttcatcgc cttttacgcc gtatttatgg ggctgtttct
      661 gaccgcgggt ctgggaagtg gttctacttt ccagatgatc gccgtcatct ttcgccagat
      721 aaccatttat cgggtgaaga tgaaaggcgg tagtgatgag caagctcaga gagaagccgt
      781 caccgaaacg gcggcagcgc tgggctttat ctcagccatt ggcgcagtgg gcggcttttt
      841 tattccgcag gcatttggca tgtcgctcaa tatgaccggc tctccggtcg gcgcgatgaa
      901 agtgttttta atcttctaca tcgtttgtgt gctgctgacc tggctggttt atggtcggcg
      961 gaagttcagc caaaaataag agatcgatgc ctgatgtaca accgtgtcgc atccgacaag
     1021 gcatcagatc ccaattcatt cctggagcag gagtcatgtc atgagtaaac ttttggatcg
     1081 ctttcgctac ttcaaacaaa agggcgaaac ctttgccgat ggtcacggac aggtgatgca
     1141 tagcaaccgc gactgggagg acagctatcg ccagcgttgg cagttcgaca aaatcgtgcg
     1201 ttccacccac ggcgttaact gtacaggctc ctgtagctgg aaaatctacg ttaaaaatgg
     1261 tctggtgacc tgggaaatcc aacagaccga ctacccgcgc actcgccctg acctgcccaa
     1321 tcatgaacct cgcggctgcc cgcgtggcgc aagttactcc tggtatcttt acagcgctaa
     1381 ccgcctgaaa tacccgctca ttcgtaaacg actgattgaa ctgtggcgcg aagcactcaa
     1441 acaacacagc gatccggtac tggcgtgggc atcgattatg aacgatccgc aaaagtgcct
     1501 gagctacaaa caagtgcgtg ggcgcggcgg gttcatccgc tccaactggc aggaactaaa
     1561 ccagctgatt gccgccgcta acgtctggac catcaaaacc tacggcccgg atcgcgttgc
     1621 cggtttctcg ccgatcccgg cgatgtcgat ggtttcttac gccgccggaa cgcgttatct
     1681 gtcgctgctt ggcggcacct gtttaagttt ctacgactgg tattgcgacc tgccgcccgc
     1741 ctcgccgatg acctggggcg agcaaaccga cgtaccggaa tctgccgact ggtataactc
     1801 cagctacatc atcgcctggg ggtctaacgt accgcagaca cgtacgccgg acgcccactt
     1861 ctttaccgaa gtacgctaca aaggcactaa aaccatcgcc attacccctg actactctga
     1921 agtggccaaa ttgtgcgacc agtggctggc accgaaacaa ggcactgata gcgccctggc
     1981 gatggcaatg gggcatgtga ttttaaaaga gtttcatctc gataatccca gcgactactt
     2041 tatcaactac tgccgccgct acagcgacat gccgatgctg gtaatgctgg agcctcgcga
     2101 cgatggtagc tacgttcccg ggcgcatgat ccgcgcatct gacctggtgg atggactggg
     2161 cgaaagcaac aatccgcagt ggaaaaccgt agcagttaat accgcaggtg aattggtagt
     2221 gccgaacggt tcgattggtt tccgctgggg agaaaaaggc aaatggaatc tggaatccat
     2281 tgccgccggt acggaaaccg aattgtcgtt aaccctgctc ggtcaacatg acgctgttgc
     2341 aggcgtggcc ttcccctact ttggcggcat tgaaaatccg cattttcgca gcgtaaaaca
     2401 caatccggtg ctggtgcgcc aattgcccgt taaaaacctg acgttagtcg atggcaacac
     2461 ctgtccggtg gtcagcgttt atgatttggt actggcgaat tacggcctcg atcgcgggct
     2521 ggaagatgaa aacagcgcga aagattacgc tgaaatcaaa ccgtacaccc cagcctgggg
     2581 tgagcaaatt accggcgtac cgcgccagta tattgaaacc atcgcccgcg aatttgccga
     2641 taccgcccat aaaacgcatg ggcgctcaat gattatcctc ggcgcaggtg ttaaccactg
     2701 gtatcacatg gacatgaact accgcgggat gatcaatatg ctgatcttct gcggttgtgt
     2761 cgggcaaagc ggtggcggct gggcacacta tgtcggccag gaaaaactgc gcccacaaac
     2821 cggctggttg ccactggcct ttgcgctcga ctggaaccga ccaccgcgcc agatgaacag
     2881 cacctcgttt ttctacaatc attccagcca atggcgctat gaaaaagtct ctgctcagga
     2941 gttactttca ccgctcgccg atgccagtaa gtacagcggt catctgattg atttcaacgt
     3001 tcgcgccgaa cgtatgggct ggctaccttc tgcgccgcag ctggggcgta acccgctcgg
     3061 gattaaagct gaagccgaca aggccggatt atcccccaca gaatttaccg cccaggcgct
     3121 gaaatcgggc gatttacgta tggcctgcga acaaccagat agcggcagca atcatccgcg
     3181 taatttgttt gtctggcgct ctaacctgct tggctcctcc ggcaaaggcc acgagtatat
     3241 gcagaagtat ctgctgggga ccgaaagcgg gattcagggc gaggaactcg gtgccagcga
     3301 cgggatcaaa ccggaagaag tcgagtggca aactgcggcg attgaaggca agctcgacct
     3361 gctggtgacg ctcgacttcc gcatgtccag tacctgcctg ttctccgata tcgttctgcc
     3421 caccgccacc tggtatgaaa aagacgatat gaacacctcg gatatgcatc catttattca
     3481 tccgctttct gcggcggtcg atccggcctg ggaatcacgc agcgactggg aaatctacaa
     3541 aggtattgcc aaagcatttt cgcaagtgtg cgtgggccat cttggcaaag aaaccgacgt
     3601 ggtattacaa ccactgctgc atgactctcc ggcagagctc tcacagccgt gtgaagtgct
     3661 cgactggcgc aaaggcgaat gcgatctgat cccgggtaaa accgcgccga atattgtggc
     3721 ggtggagcgc gactaccctg ctacgtatga acgctttacc tcgctcgggc cattgatgga
     3781 caaacttggc aatggcggta aagggatttc gtggaatacc caggatgaaa tcgatttcct
     3841 cggcaaactc aattacacca agcgtgatgg cccggcacag gggcgtccgc tgattgacac
     3901 cgccattgac gcttcagaag tgattctggc actggcaccg gaaaccaacg gtcatgttgc
     3961 agtcaaagcg tggcaggcgc tgggcgagat caccgggcgc gaacataccc atctggcgct
     4021 gcacaaagag gacgagaaga ttcgctttcg cgatattcag gcgcagccgc gtaaaattat
     4081 ctccagcccc acatggtccg gtctggaaag cgatcacgta tcctataacg cgggatacac
     4141 caacgttcat gagttaattc cgtggcgcac gctgtcggga cgccagcagc tctatcagga
     4201 tcatccgtgg atgcgtgctt ttggtgaaag cctggtggct tatcgcccgc ctatcgacac
     4261 ccgtagcgtc agtgagatgc gccagatacc gccaaacggc ttccaggaaa aagcacttaa
     4321 cttcctgacg ccgcaccaga aatggggcat tcactcaacc tacagtgaaa acctgctaat
     4381 gctgacgctc tctcgcggtg gaccgattgt ctggatcagc gaaacagatg cccgagaact
     4441 gaccattgtc gataacgact gggtggaagt atttaacgcc aacggcgcgc tgactgcccg
     4501 cgcggtggtc agccaacgtg taccgccggg catgaccatg atgtatcacg cccaggaacg
     4561 cattatgaat attcctggtt cggaagtaac tggcatgcgc ggcggcattc ataactcggt
     4621 cacccgcgtt tgcccgaaac cgacgcatat gattggcggt tacgcgcagc tggcctgggg
     4681 ctttaactac tacggcaccg tcggctcgaa ccgcgacgaa ttcatcatga tccgcaagat
     4741 gaagaacgtt aactggctgg atgatgaagg tcgcgatcag gtacaggagg cgaaaaaatg
     4801 aaaatacgtt cgcaagtcgg catggtgctt aacctcgata aatgtatcgg ctgccatacc
     4861 tgttcggtga cctgtaaaaa cgtctggacc ggacgcgaag gcatggagta cgcatggttt
     4921 aacaacgtcg aaaccaaacc gggcattggt tatccgaaaa actgggaaga tcaggaagag
     4981 tggcaaggcg gctgggtccg cgatgtgaat ggcaagatcc gtccgcgtct gggcagcaag
     5041 atgggcgtaa taaccaaaat cttcgccaac ccggtggtgc cgcagcagat tgatgattac
     5101 tacgaacctt tcaccttcga ctacgaacat ttgcatagct caccggaagg caaacatatc
     5161 cctactgctc gcccgcgttc actgattgac ggcaaacgga tggacaaagt gatctggggc
     5221 ccaaactggg aagaactgct gggcggcgag tttgaaaaac gtgcccgcga ccgcaacttc
     5281 gaggccatgc aaaaggagat gtacgggcag tttgaaaaca ccttcatgat gtacctgccg
     5341 cgcctgtgcg aacactgcct caatcccagc tgcgtggcga cctgcccaag cggcgctatc
     5401 tacaaacgcg aagaagacgg cattgtgctg attgatcagg ataaatgccg tggctggcgt
     5461 ttgtgcatta gcggttgtcc gtacaaaaaa atctacttca actggaaaag cggcaagtca
     5521 gaaaaatgta tcttctgtta tccgcgaatt gagtcaggcc aacccaccgt gtgctcagaa
     5581 acctgcgtgg gtcgcatccg gtatctgggc gtgctgcttt acgacgccga ccgcatcgag
     5641 gaagcggcga gcaccgagcg cgaagttgac ct
//
LOCUS       DADLSA010000206         5660 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.207, whole genome shotgun sequence.
ACCESSION   DADLSA010000206 DADLSA010000000
VERSION     DADLSA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.207"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            205..306
                     /locus_tag="JN691_003848"
                     /pseudo
     CDS             205..306
                     /locus_tag="JN691_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(301..717)
                     /locus_tag="JN691_003849"
                     /pseudo
     CDS             complement(301..717)
                     /locus_tag="JN691_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(762..1196)
                     /locus_tag="JN691_003850"
                     /pseudo
     CDS             complement(762..1196)
                     /locus_tag="JN691_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(1208..1429)
                     /locus_tag="JN691_003851"
     CDS             complement(1208..1429)
                     /locus_tag="JN691_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245139.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(1430..2113)
                     /locus_tag="JN691_003852"
     CDS             complement(1430..2113)
                     /locus_tag="JN691_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAY7245140.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2177..2488)
                     /locus_tag="JN691_003853"
     CDS             complement(2177..2488)
                     /locus_tag="JN691_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245141.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(2485..3456)
                     /locus_tag="JN691_003854"
     CDS             complement(2485..3456)
                     /locus_tag="JN691_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HAY7245142.1"
                     /translation="MRHGLMEAACERRIPMPNWCSNRMYFSGEPAQIAEIKRLASGAV
                     TPLYRRATNEGIQLFLAGSAGLLQITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTH
                     LQNGVLLDEQNCLMLHELWLQSGTGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNE
                     DVSVWWNRLCDNVVPEKTMPFDLLKVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYG
                     VKWPCGYDLNISSQGENFIQVDFDTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFC
                     GWQLYERGELVDVLWGELEWSSPTDDDEQPEVTGPAWIVDNVAHYGG"
     gene            3827..4786
                     /locus_tag="JN691_003855"
     CDS             3827..4786
                     /locus_tag="JN691_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY7245143.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAIIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQLALVKAMVHA"
     gene            4786..5181
                     /locus_tag="JN691_003856"
     CDS             4786..5181
                     /locus_tag="JN691_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY7245144.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            5364..>5660
                     /locus_tag="JN691_003857"
     CDS             5364..>5660
                     /locus_tag="JN691_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAY7245145.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRR"
BASE COUNT         1556 a         1481 c         1368 g         1255 t
ORIGIN      
        1 ggcacagccc gcacacgacg ctgaggctac agagctgaaa acccacaagg ccatgccccg
       61 catggccggg taaagcggag ccggcggcga cttcctttgt gctctgtccg agaccggcag
      121 gaaaaaggcg gggataatga gatggtcacc cccaccctaa cagcagtacg ctgttaaggt
      181 gaatccctta acggagagac catcatacag aacatcacac ttattggtat cgatctcgga
      241 aagcattcct tccacattca ttgtcaagac aagtctggta aggctcttct gcgtaaaaaa
      301 gatacgccgc gcatcttctg ccccccggca actgacactg catactgcat cgcgcaccgc
      361 atcaagaact gccctgattt cgtcttcggt cagccattca ccggcatacg ggaaagacac
      421 ctgcgttccg tccgccggaa gtaccggcag cgactgccac tgactgccgg acagaacaag
      481 ttccgctccg gttccggtca gtttctgaat ggcggaggcc aggtgcggcg gcagcacgcc
      541 atcgtggcgg ttaacgagta acgtcgccag ccggggcgca tccagccgca cgtaaatgct
      601 gatccagccc ggatagttcg ggttacgttg gtcaacatcc gtctcttccg gccagccctg
      661 cgccagcagc aggttacggg cgtgacgaca aatcagagca ttcagcgttt cattcataat
      721 tcatgaactc cctgtatcgc cgcccgcagg cggcgataac atcagtcgtt ttcgtgtgtg
      781 ttgtttttca gcaggctgcg ggcctgatca accatcgcct gtcggcgggt aacgtattcc
      841 gggaggggga aaccgtgatc ccgggcagcc cattcacgtt tccacagtgc ctgttcagcg
      901 atctgagtca gggtctccag cagttcagca gggacggtca cctggcggtt ttcgcgcacg
      961 ttgctgataa tctccatcgc cgcggcggaa gaaataccgg actcaatccg ctagtcttcc
     1021 gggatgtcct ccaggtgcgc caggacggtc ctgatttcat gatcggacag ggaaatatcc
     1081 attccgtcag cgagggcctg aatctcttcc ggggaccaga taaccagcat cagcggcgtg
     1141 tctcccgaat actttgctgc cagcgcccgg catagcgttt cgcatgttcc gtacatacac
     1201 tctcccctca atctttgaag caggaatcca gttccagaat ttcggtatat gcctccatca
     1261 agaggcggtt tgcgcgaaac aacgtctgac gcaccagacg ttccggcagg gtgtcagcat
     1321 taaagctgta ccgccgctcc atctcattca tggttgtacg caggtcgcac atggcattgg
     1381 ccacctccgc cacttcggcg cgaagttttt cgtgtccggc atagttcatt tacgcagcct
     1441 ccggcataaa ccagtcatca ttcgcggccc cctgctgcat ggcccgctgt acggcagcca
     1501 gtcgttgctg tccggcacgg tgatactgct caagcagctc gataccgata taccggcgtc
     1561 cggactggag ggcggcgacg caggttgaac cgctgcctgc aaacgggtcc agcactatcg
     1621 cattcgggtg tgtgaagctc tcaatcagcg gttgcaggct ggtaacgggc ttttccgtcg
     1681 ggtgatggcg gttgcccgaa tatttccagc ccaacacgtc cggcagcggg ttttgtggca
     1741 gacgtggacg gccttttgcc aggatgtagg cgcattcgtg gcgatagccc acatatgcgg
     1801 ccttcgatgt gtagtttttg gtgaagacca ggtgaccaac aacgctgaat cccgcatttt
     1861 tccaggcaga cataaagcga tcgacgcggt tccagccata gaagctcacc atcagcgcgt
     1921 cttttttgag tacgcggtac atttcattac aggccggttg cagccattca tcagttttat
     1981 caccagcaat ggtacgcccc tgacggtcac gaaaaccgac gagatatggc ggatcggtga
     2041 ggatgaaatc aatggcgtta tcaggaatac gggccataac atcgatgcag ttaccgagga
     2101 caaaacggga cataacagat ccttctctta cagagggcac ccctgtggtg cccgttcccc
     2161 gctgcctgtg ggctgttcac cgcgtccggt aacgggtcag gccgaccgtg gaataccgca
     2221 gccgtcgtct ttttgacagc aaggttatgc cgcgaaagcg gcttgaaggg cgttcatcag
     2281 aaaagggcga cgggctggcg tacactgtca ccgacgccag ccgggagcgg taaaagcgcc
     2341 cgtcccgtgc ccgccggacg cgggacactg accacaggca gcgtttatgc cgcgccgcgt
     2401 cagccggcgc agtaaaaaga ggagcacggc gacatccttt gtcggaaaag aaacgggcgg
     2461 ttgcccgccc gtctccgtca tacttcatcc gccataatgc gccacattgt cgactatcca
     2521 cgcaggtccg gtgacttccg gctgctcatc gtcatctgtc ggggaagacc attcaagttc
     2581 cccccacagc acatcgacga gctctccacg ctcatacaac tgccagccac agaaatcaca
     2641 gccctgttcg gcataccagt gctccagcgt gcagctgaaa cggcggctta attctgcaat
     2701 aacgtcgctt tccggctgac accacggcgt gtcgaaatcc acctgaatga agttttctcc
     2761 ctgactgctg atattcaggt catatccaca aggccacttc acgccatacc gttccgtata
     2821 ccagtgatat gcagaaggaa caccgttcag aacaccacca ttaaagccgt tcacttcaac
     2881 atccaggcgg gtcggcagaa ccttcagcag gtcaaacggc atggtttttt ccggcactac
     2941 gttgtcacac agacggttcc accacaccga tacatcctcg ttaccccata tgtcacacca
     3001 gtcacctctt tttgcggtga aatgcacagt gatggtttcc ctggcatcat ccggtaatcc
     3061 ttcccagcga cgctggccag taccgctctg taaccagagt tcatgcagca tcaggcagtt
     3121 ttgttcatcc agcaacacac cgttctgcag gtgtgtcagc cagcgggtga acgcgatatt
     3181 ttccgtcgat acagcaccac gtccggcagc cgtcactccg gggcactgtt cagagcggat
     3241 attttcagtg atctgcagaa gtccggcact tccggccaga aacagctgaa taccttcatt
     3301 tgtggcgcgg cgataaagcg gtgtgactgc accgctggcc agtcgtttaa tctcagcgat
     3361 ctgtgccggt tcaccagaaa aatacatacg attcgagcac cagtttggca tgggaattct
     3421 cctctcacat gcggcctcca tcaggccgtg ccgcacctgt aaccgggcga ccgcatacga
     3481 atgcaggtgc ggccccgaag ccgcagcgcg tggaggagga acgacgaaac gtgaccgggt
     3541 gaggaggccg gcgacagcga cacacttgca tcggatgcag cccggttaac gtgccggcac
     3601 ggcctggtta agtagtatcc gtctcagttc gtggcgcaaa atgagaaacc aataagaact
     3661 caaaatgaac cacaacaaga acataaagag actctaaaag agcaacaccg cttgacatag
     3721 actcaaaatg agtcacaata agaaacaata aaaaactcaa aaagatgcac cgacataaag
     3781 gatgctcaat ttgagataaa cacaaactca aaacgaggca aaaagaatgc taaaagtatc
     3841 atgcgatgat ggttcaacta acgtaaaact ggcctggctt gaagatggcg aggtaagaac
     3901 cagtctgtca ggaaacagct ttaaagaagg ctggaacccg ggactgttta atgccggcaa
     3961 agtttacaat tacgtcgtgg atgaaaaaaa atacacctat gatttgggaa gcactgctat
     4021 cataggtaca acacatgtca gttatcaata cagcaccacc aatctgcttg ccatacatca
     4081 tgcactgctg acatccggcc ttcagccaca ggatgtagaa ctgacagtaa cattacctgt
     4141 gaccgaattc tttgataatg acaatcaacc aaatgaagaa agaatagaaa gaaaaaaggc
     4201 aaatgttctc cgtgagatat ctctgaataa aggggaaaca tttaaaatta aaaaagttaa
     4261 tgtaatgcct gaatctttgc cagctgcatt tgaatcacta aaaaaagata aagtaaacaa
     4321 acttgaacgc agcctgatta tcgatcttgg aggaaccact ctggattgtg gtttaattct
     4381 cggtgcattt gaaggcattt ctgaaatccg tggctattca gaaatcggga catcccgcat
     4441 cactcatacc gtcatgaatg cgctgacaaa agcctccaca ccatgtaatt attttattgc
     4501 tgatgagtta ataaaaaaca gacacgataa tgaatatctc cagacattaa taaatgatgt
     4561 agctgaaata aaaaacattt ctcatgtaat agacagagaa gtgaaatctc tggcagaaag
     4621 catcaggcag gaaatttcaa cattctcagg aatgaaccga atctatctca ctggcggagg
     4681 agccgagctt atttaccctc acataaagca atatttcccg aatctgaaag tgaacaaggt
     4741 ggatgaacct cagttggcat tggtgaaagc aatggtgcat gcgtaatgga aagcagtgat
     4801 ccaaaaaaaa gaaagaaagt tgtggcatac ctgcacccgg cactttatcc gcaggacaac
     4861 ctgacacaac aaacaataga ctctctccca gtacaaatgc ggggagattt ctacagacaa
     4921 tcccttattt gcggagcagc tctttattct gttgaccccc gactgttaac gctaatcagc
     4981 ggttttttca gtgagaaaat cactgcagaa aatctggtga aacttataga acaaacaaca
     5041 ggatacacat ccacatcaat tgatataagt gtgttaaaaa acatcataga ggcatcttca
     5101 gaaaacaaat cagagagcat cacttcaaaa gatgatttcg aagaacaaac cagacgcaat
     5161 ctttctatgt taaaaaaata ataaaagaag agcaggttca gtcaattacc tgctcttctt
     5221 ttatacatac taaaaaatca gatttttacg aatattcaga ttatcataaa aaatgcaaca
     5281 gacaggaatg caataatttt gcattatctc gttgcttgta atgcaaatat gcattattgt
     5341 cagtgtgtga caataacaga gagatgacga tggcgaccat taacgcacgt attgatgatg
     5401 atataaagaa ccaagcagat gaagtgctga agctgatgaa tatcagtcaa actcaggcta
     5461 ttgcagcttt ttatcagtat attactgaac aaaagaaact gccatttgtg atcacctcaa
     5521 ttgtaaaaac gccgcatgat ttattacgtg aaagtacaga catgttagct gaagctcttg
     5581 cagtgatttc caatctacag gtgtggactg aacagcagga tgggattgga aaagcaaagc
     5641 taatggagta ttaccgccgc
//
LOCUS       DADLSA010000207         5613 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.208, whole genome shotgun sequence.
ACCESSION   DADLSA010000207 DADLSA010000000
VERSION     DADLSA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.208"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="JN691_003858"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="JN691_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAY7245146.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="JN691_003859"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="JN691_003859"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAY7245147.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="JN691_003860"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="JN691_003860"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAY7245148.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="JN691_003861"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="JN691_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAY7245149.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1393 a         1352 c         1524 g         1344 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatattttgc ctgaaaagtg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcagcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta acgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gca
//
LOCUS       DADLSA010000208         5586 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.209, whole genome shotgun sequence.
ACCESSION   DADLSA010000208 DADLSA010000000
VERSION     DADLSA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.209"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..200)
                     /locus_tag="JN691_003862"
     CDS             complement(<1..200)
                     /locus_tag="JN691_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HAY7245150.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLS"
     gene            complement(487..1212)
                     /gene="phoU"
                     /locus_tag="JN691_003863"
     CDS             complement(487..1212)
                     /gene="phoU"
                     /locus_tag="JN691_003863"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAY7245151.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(1227..2000)
                     /gene="pstB"
                     /locus_tag="JN691_003864"
     CDS             complement(1227..2000)
                     /gene="pstB"
                     /locus_tag="JN691_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAY7245152.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(2092..2982)
                     /gene="pstA"
                     /locus_tag="JN691_003865"
     CDS             complement(2092..2982)
                     /gene="pstA"
                     /locus_tag="JN691_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAY7245153.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2982..3941)
                     /gene="pstC"
                     /locus_tag="JN691_003866"
     CDS             complement(2982..3941)
                     /gene="pstC"
                     /locus_tag="JN691_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAY7245154.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(4028..5068)
                     /gene="pstS"
                     /locus_tag="JN691_003867"
     CDS             complement(4028..5068)
                     /gene="pstS"
                     /locus_tag="JN691_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAY7245155.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(5316..>5586)
                     /locus_tag="JN691_003868"
     CDS             complement(5316..>5586)
                     /locus_tag="JN691_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7245156.1"
                     /translation="TSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTD
                     MGKSTVYGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         1384 a         1475 c         1353 g         1374 t
ORIGIN      
        1 tgacttaaga gttcgcttaa tcgcccgttc agttcatgac tgctcaaggc atactctttt
       61 tctattccac ttgagttaat tctttcgcca gcgcgttttt gaaagccaat tccgcgcccc
      121 atgacgactt tttcccgctg ttgatcatca ataaccacca caacattatt gttgagaatt
      181 ttggtgattt gcatgttcat agcaaggacc ttttttataa acaaaaaaaa cccgacttca
      241 ccagtattct ctggttatgt caggttttgc ctgcgaatgc agtaacaatc cagtcattta
      301 ttaatggttt ttataacgaa catccaggtt cggaaattaa tttaattgcg tgcagagaaa
      361 atagcaatgc gctattgata aaaatatgac catgctcgca gttattaact ttgtgtaatt
      421 ttaggaattt ataaagttat atataacaaa tcccaataat taagttattg ggatttgtct
      481 ggtgaattat ttgtcgctat cttttcccgc cagcagttta tccagctcat caccgcctac
      541 atgacggaaa tcctgaccct tcacgtagta gaagataaac tcacaaatat tctggcagcg
      601 gtcgccgata cgttcgattg aacgcgcaca gaacagcgcg gtaagtacgc tcggaatagt
      661 gcgcgaatct tccatcatgt aggtcatcag ttgacgaaca ataccttcgt attcctgatc
      721 gactttttta tcttcacgat aaatacgtac cgcttcgtca atgtccatcc gcgcgaacgc
      781 gtccagcacg tcgtgcagca tctggatggt atgacggccc agcgactcca gacttaccag
      841 caacggctga tgctgctggg agaatttctc cagcgcagta cggcagattt tgtccgccac
      901 gtcgccaata cgctccagct cggcaatggt tttactgatc accataacca gtcgcaggtc
      961 gctcgccgtc ggctgacgtt tggcgataat gcgcacgcag gcttcatcga tcgccacttc
     1021 catcatgttg acgttcttgt cgccttcgat cacgcgcttc gccagatcgc tgtcctggtt
     1081 atgcatcgcg gtgattgcat cagaaagctg ctgctccacc atgccgccca tggtcatcac
     1141 ctgcgtgcgg atactttcca gttcggcgtt gaactggccg gaaatatgtt tattaagatt
     1201 gagactgtcc ataacgcact cctgaatcaa ccgtaacgac cggtgatgta gtcttctgtt
     1261 tgtttcttcg ctggcttggt gaacagatcg tccgtgttgc tgaactcaat caattcgccc
     1321 aagtacataa atgccgtgtg gtcggaacaa cgcgcagcct gctgcatgtt gtgggtgacg
     1381 atcaccacgg tgtaatcctg cttcagttcg gtgatcagct cttcaatacg cccggtagag
     1441 atggggtcga gcgccgaaca cggttcgtcg agcagcagca cttccgggcg aatggcgata
     1501 ccacgcgcaa tacacagacg ctgttgctga ccaccagaga gagagtaacc gctctggtgc
     1561 aatttatctt tggtttcgtt ccacaatgcg gctttggtca atgcccactg cacgcgctcg
     1621 tccatgtcgg cacgggagag cttctcaaac agacgaacgc caaaggcgat gttgtcgtag
     1681 atggacatcg ggaacggcgt cggtttctgg aacaccatgc ccactttcgc acgcagcagg
     1741 gcgatatcct gagagttggt caggatgttg tcgccatcaa gcagaatttc accttccgca
     1801 cgctgctccg ggtacagttc aaacattttg ttgaaggtac gcagcagcgt cgatttaccg
     1861 cagccggacg gcccgataaa cgccgtaacc tggtttttgg cgatatccag gttgatgttt
     1921 ttcagggcat ggaatttgcc gtagtagaag ttcaaattac gaacctgaat tttactcggg
     1981 gcagtttcaa ccatactcat tgcaatcttt tcctcatcgt acctgatgct tttcgctaat
     2041 caggcttacc gtagggccgg gtaaggcgtt cacgccgcat ccggcaaagt gttaaccgtg
     2101 tttattcttc gcaaaaacaa cgcgcgccag aatgttcagc agcaggacgc acagggtaat
     2161 gatcaatacc ccggcccagg ccagttgttg ccattccgca aacgggctca tcgcaaactt
     2221 aaagatcgtc accggcaggt tggcgatcgg ctgcatcatg tccgtgctcc agaactggtt
     2281 ggagagcgcg gtaaacagca gcggcgcggt ttcaccggca atacgggcaa tcgccagcag
     2341 gataccggtc ataatcccgg acaccgacgc tttcagcgta atcgcggaga tcatcttcca
     2401 cttcggtgta cccagcgcat aagccgcttc acgcaggctg tccggcacca gtttcagcat
     2461 gttctcggtg gtgcggataa cgatcggcac ctgcaacagc gccagcgcaa ttacgcccgc
     2521 ccagccggag aagtgctcca tctgtgccac cacgatggtg tagacaaaca gaccaaccac
     2581 aatcgacggc gcggagagca gaatgtcgtt aatgaagcga atcacttctg ccagccagga
     2641 tttacgacca tattccgcca gataaatccc cgccataatg cccagcggcg taccgaatac
     2701 cgtggcccac aaaattaaca gcccgctacc cgccagggcg ttcgccagac caccaccttc
     2761 cgtattaggc ggcggcgtca tttcagtgaa cagcgccagc gacataccgt cgataccgcg
     2821 agtgatggtg gacattaaaa tccagatcag ccagaacagg ccgaaggcca tcgtcgccat
     2881 cgagagcgtc agcgcaatac ggtttttgag gcggcgacgc gcctgcattt tgcggcgaga
     2941 ttcagccagc gccgcagtgg tttgcatttc aaccatagcc attagcgtgc cccctcattc
     3001 ttagccaggc gcataatcat aaacttcgat gcggcgagga cgataaaggt aatcacaaac
     3061 aggatcaggc ccagttccat cagtgcggca acgtgcagac cggattccgc ttccgcaaat
     3121 tcgttcgcca gcgcagaggt gatactattg cccggcatgt acagcgaggc gctgtcgagc
     3181 tggtaggtgt taccgatgat aaaggtcacc gccatggttt caccgagcgc gcggcccagc
     3241 cccagcatga tgccgccgat aacaccattt ttggtgaacg gaagaacgat acgccagata
     3301 acttcccagg tggtgcagcc aataccgtag gccgactctt tcatcatcac cggggtttgt
     3361 tcgaacacat cacgcattac cgccgcaatg tacggaataa tcatgatggc gaggatcacg
     3421 cctgccgcga ggataccgat accaaatgcg gggccagaga acagcgcgcc aacaatcggg
     3481 atattcgaca tgatattgcc gaccggctcc tgaaagtaaa cggcgaacag cggcgcaaag
     3541 ataaacaggc cccacatgcc gtaaacgata cttggaatgg ctgccagcag ctcaatggcg
     3601 atacccagcg ggcgtttcag ccagccaggc gcaagctcag tcaggaacag ggcgataccg
     3661 aaactcaccg ggacggcgat cagcagcgcg ataaacgaag tcaccaacgt accgtagatc
     3721 ggcaccagcg ccccgtagat atcgttcggt gcatcccact ctttggtcca taggaaagcc
     3781 agaccaaatt tctgaatgct cggccaggag gagatgatca gagagacaat aatgccaccc
     3841 aacatcaata gcacaatcag cgccgccagt tttaccagca cgctgaaaat tatgtcgccc
     3901 tttttacccg gtgggttaaa agcgggcttg gttgcagcca taagttactc ttcagttaaa
     3961 cgcgtttaca aagtcatttt gtaatgccgg atgcggcgta aaacaccgta cccggcctgg
     4021 agttttatta gtacagcggc ttaccgctac tgtctttaat attggtcttc cacgcagcgc
     4081 gaacctgttc aactacacta tccggcaggc tggcgtaatc caggtcgttc gcctgtttag
     4141 ccccggtttt gtacgcccag tcgaagaatt tcagcacctc tgtgccttgt tctggtttct
     4201 tctgatcttt gtggatcaga atgaacgtgg tagaggtaat aggccatgca tcttcgcctt
     4261 tctggttggt cagatcctga gcgaaggttt tgctccagtc tgcacctttt gctgcattag
     4321 cgaagttttc ttcggtcgga ctaaccggtt taccatcagc ggagatcagt ttggtgtacg
     4381 ccaggttgtt ctgcttcgcg taagcatatt caacataacc aattgcaccc ggcagacgct
     4441 gaacgaacgc ggcgataccg tcgttacctt taccgcccag accgatcggc cattttacgg
     4501 tagagccagt accaacgttg tttttccact cttcgttcac tttcgccagg tagctggtga
     4561 agacgaagga agtcccggag ccatctgcgc ggcgtactac agcaatgttt tgtgaaggca
     4621 gtttcagacc cggattcagt ttggcgatgg cttcatcatc ccacttcttg attttgccca
     4681 ggtagatgtc gccgagggtt ttaccatcca gcaccagttc gccagacttc agccctggaa
     4741 tgttaaccgc cagcaccacg ccgccaatca cggtcgggaa ctggaacaga ccttcctgag
     4801 ccagtttttc gtcagacagc ggcgcgtcag aggcaccaaa atcaacggta ttagcgataa
     4861 tctgttttac gccaccggaa gaaccgatac cctggtagtt aactttatta ccggtttctt
     4921 tctggtaagt gtcagcccat ttggcataca ccggcgcagg gaaggttgca cctgcacctg
     4981 tcaggcttgc ttctgcaaac acagagaaag cactcatcga taaggtcgcg gcgacaacag
     5041 ttgcgacggt ggtacgcata actttcataa tgtctcctgg gaggattcat aaagcattgt
     5101 ttgttggcta cgagaagcaa aataggacaa acaggtgaca gttatatata aggaatatga
     5161 catttttatg acagaaagga attaccaaat aacttactga ttttaaatgt gaagatgaaa
     5221 atcccacatg ccatcttttt gtcatgatgc atttaaaagt gataaagaaa tgggtgagat
     5281 gacaacgatg tgataaacaa tcgttgtcag acctattagc gcacaatctc aacagttatc
     5341 gtcgctgttg cagtaaattg ccccggggct gggcgagctc cagtaaggct cgcaggagca
     5401 gctgaaaaag ttacactacc tgatttttca ccatacactg ttgatttacc catgtctgtt
     5461 ggcaactcac caccgttcac tttgatttcc ttattatttt tgtcgtaaat ttttatcccc
     5521 acgttagagt ttgaagttac gaccattgaa ttgtccgtat tgggttcggt agcataaaaa
     5581 gacgta
//
LOCUS       DADLSA010000209         5580 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.210, whole genome shotgun sequence.
ACCESSION   DADLSA010000209 DADLSA010000000
VERSION     DADLSA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.210"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            1..1160
                     /gene="espX1"
                     /locus_tag="JN691_003869"
                     /pseudo
     CDS             1..1160
                     /gene="espX1"
                     /locus_tag="JN691_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
     gene            1482..2015
                     /locus_tag="JN691_003870"
     CDS             1482..2015
                     /locus_tag="JN691_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7245157.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSEMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            2065..2748
                     /locus_tag="JN691_003871"
     CDS             2065..2748
                     /locus_tag="JN691_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7245158.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVPFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAEKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKNNA
                     ISINNETANWITVTTIKAQNVKVNNESILLPPFSNNDITLKNNHASEYELTVVDDYGN
                     NIHSKIAAR"
     gene            2761..5212
                     /locus_tag="JN691_003872"
                     /pseudo
     CDS             2761..5212
                     /locus_tag="JN691_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            5225..>5580
                     /locus_tag="JN691_003873"
     CDS             5225..>5580
                     /locus_tag="JN691_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HAY7245159.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCGLTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLLETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDDKKIEL
                     PGKSSYNLKEMLSAIN"
BASE COUNT         1924 a         1065 c         1013 g         1578 t
ORIGIN      
        1 tcatagaaca tacagttcaa ttttcttttt ttaaacacaa aggcatcaca ttacttagat
       61 tggatgtttt tgatgacaga acaagcgaat gtctttctga agagattaag atatatcaag
      121 aatgccatga aaaatttatt aaatttttaa aggcaaactt caaccaggaa atatacccag
      181 agctatacac tcctgaaata ttttatgagg cctgcagaaa tctccagtca ttttatgatc
      241 atcaggagtc gtcacaaaac gctaaataca gtgcaatagt caagaaaaaa agttatttta
      301 ataaggaaat tagaaatcta ataaaaaaga atatttatcc tgagctatac aatgagcaat
      361 gcaataaaat ccccgcctca tcgacagatg ataaccagaa aataacatgg cagaatttta
      421 aaactagcaa tgcggcatat tcacaactat gtgaaaaatt atctttatta aaaagctcgc
      481 ccagtcggtt aatagagaaa tcagcttact gctcaaatga aaacatgatc accgataaat
      541 ttaatgttgt cttttcttac tgcggagata atgtaaatga atttatactt ttattaccct
      601 ataataaatc tctggagatg catgaactta acgagcaaaa catccagtat ttgacttccc
      661 ttaatattaa tatacataaa atgttgctat caaatataac aatagaaaag agcaaccttt
      721 catatgggta ttattttggt tgtgtattat ccaatatatc atgttttgaa agcgacttat
      781 caaacaccat cttttcaaat ggagaaataa acaatccctt cattaaaaaa agcaacatat
      841 ttggaacatc gttcactaat accatgataa aaaatttgcg ctgtgaagat attatgcctg
      901 gccgatggac cactcaactt gtcaataagc acttaggtta cagatacaca ggagtattca
      961 aaaccctggc aagtattgat gacaaaccgt cccgtttcga aattttgata ccattggtac
     1021 aaactttagt tcgcgacaat gtaaagctaa ataatgatgt gtataaagaa ctgaaaaagt
     1081 ttatgcacga ttacgacaaa acatccccag aaatgagaaa ataccttcag agcatcaacg
     1141 agtctatgtt ccttataaaa aaatatctca tcaagattaa aacaccccaa aaaataagta
     1201 atttcagaat gaaactacct gtgataatta ttaattaact gcagagaaat aaaaaataat
     1261 ctgaagcatg ctctgaaaca tcctactctg tcactctaat gatggcacct tgctcttctt
     1321 ataaagaagg gaataattaa gaatattaaa ttttcaaact ttcatcaact tcacatcaag
     1381 aaattaaaaa atttaaacat taatctaaat aataacaacc tgatcacata atcgggtttg
     1441 aaaacgagtg accttccata ccagttaatt aaggatgatt tatgaaaaag tatattattc
     1501 ccttcgttgc gacaacgttg atgtttagcg caacaacctt cgccaaggat gtcgatcaag
     1561 gaacactaac cattagagga gaagttgttg atacaacgtg ttactttgtt aatggtgatt
     1621 cgacaacaga tatcgttgtc gaagatgttg taaccagtga aatgaatcaa cttatcaata
     1681 atgaaatcta tactttaaaa accggttcaa cctctcatga cttagaaatt cagtgtgacg
     1741 gaaatacagc cccaagtatc gaagtactag catcagagtt tgatgctaat aactttacgc
     1801 tgaataaagg aaccgctgag aacattggct ttgccatttt cctcgatgat gggactttga
     1861 ccagaatgag accagggtta aaaacaccgc tgaaagataa tggctctggt caatattttc
     1921 ttaatttgaa tgcacaatat gcccgcacaa gtggcaatcc agttacaaaa ggctccgtag
     1981 agtcaaccgt aacgattaaa atcagtgcag attaacgttt tttgccggta gctctgctac
     2041 cggtattatt tacggagtga ttacgtgaga ataataacag cattactgat gtcatttttt
     2101 attttacctg ttcatgccgg tgtggtcatc tatggtacgc gcattattta tcctgaaaaa
     2161 aacagagaag ttctcgtaca gttaatgaat caaagtaaaa atgcctcttt aattcaggca
     2221 tggatcgatg atgggaacac cactatcgcg ccagaaaaaa ttcaggtccc atttattctc
     2281 acaccgccag taagtcgtgt agcaggtggt tctggccaac aacttaaaat aagaaaaatg
     2341 ccaaacaact tgcctcacaa taaagaaagt ttattttatc taaatgttct ggatatccct
     2401 ccaaacaacc cacaaaatgc agaaaaaaac aaaattaaac ttgcattgca aaaccgtata
     2461 aagctacttt ggcgtccaag cggaatagct ccggtagata aaaaaagcct ttctcaatta
     2521 aatatcaaaa agaaaaataa cgccatctca ataaacaacg aaacagccaa ctggataacg
     2581 gttacaacta tcaaagcaca aaacgtgaaa gttaataatg aaagtatctt actgcctcca
     2641 ttttcaaata acgacattac attaaaaaat aaccacgcat cagaatatga actaacggta
     2701 gttgatgatt acgggaataa catccatagt aaaatcgccg cgagataaca ggaaaaagtc
     2761 atgttaagga cgacacgatg ggtcgctgct attatttttt tgtattcttt tccgggttat
     2821 gctgaagaaa attttgatac gcactttatg agtggcggaa tgagaggaga aaaagtttcc
     2881 gaatatcgtt tcgataataa acaaccgctt ccaggaaact acgaacttga tttttatgta
     2941 aataaccagt ggcgtggaaa acaagatatc acgatcccag agtcaccagt caaaccatgt
     3001 ctgcctaagg tactactaac caaactgggg gtaaagaccg gtaacctcaa tacagaagat
     3061 aattgtattt tactggatga agcggttcat ggtggacaat atcagtggga tatcagtgaa
     3121 caccgactaa atttaacagt accacaggcg tatatcaatg aacttgaaag aggctacgtt
     3181 cccccagaaa gttgggatcg cggcattgat gctttttata cctcctataa tttaagtcag
     3241 tatcgctctt atgacagcaa taataacagt catacagcca gttatggccg gtttaacagc
     3301 ggattaaatt tatttagttg gcagttacac tctgatgcca gttatagcaa acctgatgat
     3361 atgaaaggca aatggcaaag taatactctt tacctggaac gtggctggtc gcagatatta
     3421 agcacagttc agataggaga aaattacaca tcctcactca tattcgactc tctgcgattt
     3481 agcggaatac gcttatttcg cgatatgcaa atgctacctg attctatgca aagctttaca
     3541 cctttagtac aaggtgttgc gcaaagtaac gccttgataa cggtttcgca aaatggctat
     3601 accatttacc aaaaagaggt tcccccagga ccatttacta tcgcagattt gcaactttcg
     3661 ggcagcggct ctgatctcga tgtcagcatt aaggaagcag atggtagtgt tcgttccttt
     3721 ttggtgccct actcttccgt gcccaatatg ctgcaacctg gtgtttcaaa tttcgatttt
     3781 atcgccggac gtagtcagat atatggtgtc aagaatcagg aggactttct cgaggcaaat
     3841 tatatatatg gcctcaacaa tcttttgaca ctttatggcg gtacgatttt atccgataac
     3901 tataatgcta taactctggg aaatggttgg aatacaccgc tgggagcaat ctcttttgat
     3961 gctacccgat cgagtagtaa gttaaataat gataccagac acgaaggtac gagctatcag
     4021 gtcgcctata ataaatatct attgcaaacg gccactcact ttagcgtggc cgcctagcgt
     4081 tatgcttcgc aggattacag gacattcagc gaccatcttt acgaaaataa taaaataaat
     4141 catcagagtg actatgatga tttttatgat attggcagaa aaaaatagcc tttctgccaa
     4201 tataatgcaa cctttatcca ataatctggg aaatgtatca ttaagtgcac tttggcggaa
     4261 ttactggggg cgaagtggaa atgctaaaga ttaccaattc agttactcca ataactggca
     4321 acacattagt tatactttct ctgccagcca atcctatgat gaaaacgata aagaagagga
     4381 gcgttttaat ctgtttatct ccattccttt ctactggggt gatgatattg ccaaaacacg
     4441 tcaccaaatt aacttatcga attctacctc attttccaaa gatggctatt cctccaacaa
     4501 tactggaatt actggcatag ccggtgaaca tgatcagtta aattatggta tatatgttaa
     4561 tcagcaacaa caaaataatg atacctcgct tggtacgaat ttaagctgga gaactccaat
     4621 cgccacaata gatggcagct atagccattc taaaaacgcc tggcaaagtg gtggaagtat
     4681 tagtagtgga ttagttgtct ggcccggtgg tattaatatc actaaccagc tatccgatac
     4741 atttgcaatt ctggatgcgc ctgggttaga aggcgcgcat attaatggac aaaaatacaa
     4801 ccgaacaaac agcaaaggcc aggttgttta cgacctgatg atacctcatc gtgaaaacca
     4861 tctggtactt gatatagcaa acagtgaaag tgaaacagaa ttgcagggca atcgtcaaat
     4921 tattgcgcct taccgtggag cagtttctta tgtgcagttt acaactgacc aacgtaagcc
     4981 ctggtatata caggcactgc gtcccgatgg ttcgccatta acctttggct atgacgtact
     5041 ggatctccag gaaaacaata ttggagtcgt tggccagggt agtcgccttt ttattcgcgt
     5101 agatgaaatt cccactggca taaaagttgc tctcaatgat gaacagaatt tattctgtac
     5161 tattactttt caacacgtta tcgatgaaaa caaaacatat atatgccagt aaaaggagtt
     5221 tactatgaag tggcttttat taatcacctt gagtctatat tcttttattg tgcaaagtgc
     5281 tccctgtgga ttgaccaatg tcggggaaca acgtggaaca tatatactca agccactgag
     5341 tatgaaaggt aatctcaccg ccgaaaaaat tgaaaacacc ggaaatatca ttgagtgtgc
     5401 agacttgcta gagacgacta acgccattaa agttcatttt ttaacacaga acgctttaag
     5461 gtttaaagtc ggaagtcgtc attatattat taatttcccc tttgaatctg acgataaaaa
     5521 aatagagctt ccagggaagt catcttataa tttaaaagag atgctatctg cgataaattt
//
LOCUS       DADLSA010000210         5578 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.211, whole genome shotgun sequence.
ACCESSION   DADLSA010000210 DADLSA010000000
VERSION     DADLSA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.211"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..498)
                     /locus_tag="JN691_003874"
     CDS             complement(<1..498)
                     /locus_tag="JN691_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245160.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLS"
     gene            complement(506..2854)
                     /locus_tag="JN691_003875"
     CDS             complement(506..2854)
                     /locus_tag="JN691_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7245161.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEVIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(3032..3943)
                     /gene="gsiD"
                     /locus_tag="JN691_003876"
     CDS             complement(3032..3943)
                     /gene="gsiD"
                     /locus_tag="JN691_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAY7245162.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(3946..4866)
                     /gene="gsiC"
                     /locus_tag="JN691_003877"
     CDS             complement(3946..4866)
                     /gene="gsiC"
                     /locus_tag="JN691_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAY7245163.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(4884..>5578)
                     /locus_tag="JN691_003878"
     CDS             complement(4884..>5578)
                     /locus_tag="JN691_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="with GsiACD is involved in the transport of
                     glutathione into the cell; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAY7245164.1"
                     /translation="PKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKP
                     WPYDPVKARELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMD
                     AGQRAAEVEGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNK
                     QVDDFLAQALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMP
                     DTGFSFEDADLQ"
BASE COUNT         1581 a         1443 c         1183 g         1371 t
ORIGIN      
        1 tgacagtaaa gaaaaacgct ccagaccaag caaaaatatc agactacgat tgtgagttac
       61 cggatagagg taatagacga aggttttacc gtcaaaagag ataaaccctg tatttttgac
      121 atgtcgatac ttcacaacct gcataatatc tgcacaataa tttttctcga actcctttac
      181 ctgaatacat tcgtcactgg aaatcagata aggaccgaca tcgtacaaat aagaggggga
      241 aaccgagtcg gcgtaagctg tgcgcaaacc aaccaacagc tgttcctccg tgggatttag
      301 cgtgttgata agatcaattg ccacgctacg ccgatattca gcaatatttg ccaccgcaaa
      361 agcagtatca ataacggact gtttcaggct atacacccgt tcctttacca tttgagtgta
      421 gatcccgcag gcaactgctg aaatcatgat aaaaatcagc agactgactg taagtacgaa
      481 cttattgatt ctggacatac gtaaactact cttttactaa ttttccactt ttatcccagg
      541 cggagaatgg catgggtttc tggtacagat acccctgagc ctgaacacat cctaatttaa
      601 ttaattcgcg cgcaacgtct tcgctctcta cgccttccgc aatcaccggg atccggttat
      661 agcgtgccag atcgaaaata atgcgcacaa tttttcccgc agtaccgaaa atatcgccgg
      721 taaattgcga gtccaccttt atgctgtctg gattaatttc ataaaaacgt ttcaggtttg
      781 acaaaccggt gccaaagtca tcaatcgata tccgtacccc ttgtttacgc agttgttcaa
      841 tattgagata aaaatgttca ttaattcgct cgatcacggt ttcagtgatc tcaacacaga
      901 ggctgttcgc cttcaggcca ttttctttca tgatggtggt aaaacgctca tgaaagtgcg
      961 gttcgtttaa ctgccagggc gaaacattaa tatgcagtaa gaaatcatcc ttcagcgacg
     1021 cacgttgact aaaatgtctg aattcaccca ttgccgtttt aataatctga taacccagct
     1081 cattgataaa cccgatatct tcagcaatgg taatgaactt cagcggtgaa atgatccccc
     1141 gctccgcaga aacccaacgg cacaacgcct ccgcgctcag aatggcgcgg gtgttaatat
     1201 caacgatcgg ttgatagacg aggaatagtt cgccaatttc cattgcatgg cgaatattac
     1261 gcgcaatcca gatattctct tctatttcgt tgagtttcgc gtcatcacag aaaataaact
     1321 cggagaacat atggtcacgg atactggaaa tcgccagttt cagtttttct gcgtaactcc
     1381 aggcgttttc cccctgcaaa ggttcacaaa tggcagactt atggatgcgc agattgcggt
     1441 agccaaagct gctgtcaaaa tcgtttacca tcgctatttt acgggtaaat gtctgtacat
     1501 tttcccgaca cagaagtaaa tagagatcgg cattgaatgt cacgatatcg cagcattgcg
     1561 catatttctc ggcaattcgc tgacacaatt tcctgacgat tttattgcgt tcatcaacac
     1621 caaacaccgc ttccacggct tccagaccac acagtttgat gatccccaca gccagatact
     1681 gattattgtt ggactcaata atcgccatat ttttaataaa accgctttcc tgatcgtaag
     1741 agatcagtga gagcaattta tttgaccgtt caatttcggc atctttcatt tccgcgatcg
     1801 aatttttgat gctgataatc tctttcgcat cgccttcgcg aattttcaga gcctcgatct
     1861 gatccgttga gtcgggcaaa aacgccagcg tattaaccag ctcggtcatt tcagaaaatt
     1921 gccgacggaa cacacgagtg gtcatgaaat ataccaccag caacacgata ataatgaacg
     1981 caaacggtag ataaaaaagt ttatctgatg cgttcaataa attaccgcca ggataataaa
     2041 aatagatttc gaacaatgca ttatttggca ctgctgatgt ctttgcaata tactccgtat
     2101 cgacagaatc aataatatta taattacctt cgctattata aataatcagt ttttcacgag
     2161 cgagttgatt cttggcaatg acctcatgac cttgtttcaa aaatagaact tcatcattag
     2221 cgctgatttg atcaagatac tcgttcagat accgccagtt taaatcgaca tgaattttat
     2281 ctaccgctaa caaatcgtga tctttgccgt tgatacgtag cgtaaaagag atacattttt
     2341 tacccggacg atcaaagcaa ttgtatgccg gattccagct gttgttagag tcatctgaag
     2401 ccaccgacca cggtcgtttc gatgggttaa aatcggcaaa aataacccgc cctgactcgt
     2461 cgacaagcgg tccatttact tgttcctgat gataaacaac gattttatca ttcgccctcc
     2521 gcgaaaagag ataatattta ttatcatcaa gtaccagtcc caccgagtct aaaaatggca
     2581 tcatttccat tttcagaatc acggcgcgtt gaatctcctc tgtatttttt aataaataca
     2641 gtgagtcctt taatgtcagg gccgaacgtt caatatcttt aaagaaatag ccaacgtttt
     2701 tctgaataag ttcaaagcgg ctatcggcac gttttaccgc gcgttccgat accagttgat
     2761 agttaattat gaaaaaaaga ccaataaatg acagtgctgc caataaaaat aaaattatca
     2821 gccttatctt tatcttttcg tataaactca gcatgttaat acatttaaca aaataactat
     2881 ctgaacgctg tgccgctgca aaaatagtcc cttgctgcgg ctttttaacc aaattacccg
     2941 attctacaaa atcagcagaa agttacaaca cttgcttagg gcaataattt cctgtgaggt
     3001 gattaccctt tcaagcaata ttcaaacgta actatccttt aattttcgga tccagcgcat
     3061 cgcgtaaacc atcgcccaac aaattgaacg ccagtacggt cagaaaaata gccagggccg
     3121 gaaaaacagc gacatgcggc gcgataacca tatccgctcg tgcctcattg agcattgctc
     3181 cccactctgg tgtcggcggc tgcgcaccga ggccgagaaa tgacaggctg gcggcagaga
     3241 taatcgaggt gccaatgcgc atggtgaaaa acaccacgat agaagagacg gtcccaggca
     3301 ggatatgacg caataaaatg gtcatatcgc tggcaccaat actgcgtgct gactcaataa
     3361 aggtttgctg tttcaacacc agcgtgttgc cgcgcaccag gcgggcaaac gcggggatgg
     3421 aaaaaatggc gactgcgata atcacgttag caatgccgct tcccaacacc gcaacaacag
     3481 cgatcgccag taaaataccc gggaaggcaa acagcacatc gcaaatgcgc atgatcagcc
     3541 gatcccacca gccttcataa tatccagcca acaagcccag caacgtccct atcgccacac
     3601 cgataaacac ggcaaacacg cccgccgcca gcgagatttg cgcaccaacc aggacacggc
     3661 tgaaaatgtc gcgccccagt gaatcgacgc caaaccagtg ctgcaaagaa ggtccgttat
     3721 tcagattgtc ataatcaaaa taattttcgg cgtcataggg agcgatccag cgtgcaaaaa
     3781 tggccaccac aatcaataaa ataacgaata atgcggcggt catcgccata tgctgacggc
     3841 gaaatcgtcg ccagaattca tgccacggtg tacgtacctg gtcaggtttg accagtggca
     3901 tggcgtttaa caccgcctga cgtcgccagt taaatagtcg catccttact tgtacctgat
     3961 agccgggtta atggcggcgt aaagcacatc caccactaag ttgataagaa taaattccag
     4021 cgagaaaagc agaatttccg cctgaatcac cgggtaatcg cgcatttcta cagagtcaac
     4081 gagtaagcgt ccaagtccgg gccagttgaa aactttctcc accacgatag aaccaccgag
     4141 caaaaagcca aactgtaagc ccatcatggt cactaccggg atcatcgcgt tgcgtagccc
     4201 gtgtttgagg acaacccagg tttcgctcac ccctttcgcc ctcgcggtac gcatataatc
     4261 ttcgcttaaa acgtcgacaa atgacgcacg ggtaaagcgc gccatcacgg cggccactgc
     4321 cgcgccgagc gtcagggagg gtaaaatgta gtgctgccaa ctgtctgctc ccacagtagg
     4381 cagccagccc agttcaacgg agaatacctg aattaaaagc atccccagcg caaatgccgg
     4441 aaacgagatc cccgacaccg caatggtcat actcaatcga tccggccaac gattacgcca
     4501 gacggcggcg ataattcccg ccgccatacc aaatatcacc gcccagacca tactggttat
     4561 ggtcagccac agcgttggca taaagcggct ggcaatctca tcggcaaccg gacgacgcga
     4621 caccatcgac aggccaaaat ccccctgcac agcattgctg atatagtgcc agaactggtg
     4681 atacagcggc tgatccaacc ccagctgctg acgcaccagt tctataacct gcgcatcagc
     4741 ttcgggcccg gcaatcaatc gcgccggatc gccgggcagc atgtggacaa ataaaaacac
     4801 cagcaccgag acgataaaca gcgtcggaat caaccccagt aatcgtttga taacgtaatt
     4861 aagcattcca ctccctgcgt tgcttattgc aaatccgcgt cttcaaagct gaagccggtg
     4921 tctggcatga tccaaaaacc ggtcaggttt ttactgtgtg ccgacaccag tttttctacc
     4981 accagcggga tccacggcga ttcttgccag atgatatcct gcgccgcctt atataagcgg
     5041 gtcttttccg ccggatcatt cgttttcagt gcctgagcca ggaagtcatc cacctgttta
     5101 ttgctgtaaa acgcggtatt aaacagcgtc ggtggccagt tttgcgaggc aaacagcggc
     5161 gatagtgccc agtcggcttc gccggttgaa gccgaccagc cagtgtagaa catccgcacg
     5221 ccgctctctt tttgcccttt accttcaact tctgccgccc gctgtccggc atccatcgcg
     5281 gtcacctggg ctttaatccc gacctgcgct aactgctgct gggtaaattg cagcactttc
     5341 tgcgcggtgc tgtggttatg tgacgaccac agcgtggtac tgaaaccgtt gggatatccc
     5401 gcctctttca gtaattcgcg cgctttcact ggatcgtaag gccacggttt ataactttgc
     5461 gcgtaggcga tacttggcgg taccacacca gtagctggcg ttgcatagcc cgcaaaggca
     5521 actttcacca gcgccggacg gttaatggcg taattcagcg cctcacggac cttcgggt
//
LOCUS       DADLSA010000211         5445 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.212, whole genome shotgun sequence.
ACCESSION   DADLSA010000211 DADLSA010000000
VERSION     DADLSA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.212"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..185
                     /locus_tag="JN691_003879"
     CDS             <1..185
                     /locus_tag="JN691_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7245165.1"
                     /translation="GMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNI
                     NAVAMKITADIMQHAD"
     gene            complement(339..1988)
                     /locus_tag="JN691_003880"
     CDS             complement(339..1988)
                     /locus_tag="JN691_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAY7245166.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2139..3488)
                     /locus_tag="JN691_003881"
     CDS             complement(2139..3488)
                     /locus_tag="JN691_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7245167.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTVFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFVTFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4194..4658)
                     /gene="soxR"
                     /locus_tag="JN691_003882"
     CDS             complement(4194..4658)
                     /gene="soxR"
                     /locus_tag="JN691_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAY7245168.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4744..5067
                     /gene="soxS"
                     /locus_tag="JN691_003883"
     CDS             4744..5067
                     /gene="soxS"
                     /locus_tag="JN691_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAY7245169.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5070..>5445)
                     /locus_tag="JN691_003884"
     CDS             complement(5070..>5445)
                     /locus_tag="JN691_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7245170.1"
                     /translation="MTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSF
                     VETLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKA
                     MPPQVFMQWMEQLPARELTRGQ"
BASE COUNT         1460 a         1525 c         1309 g         1151 t
ORIGIN      
        1 agggaatggg catttccatt ctccgtgggg aggaaaatat ccccattgat agtgataccc
       61 tggtaacact acagaaattc tttgaagaag attgtacctc tcatactggc atgtcacaaa
      121 ctgaggataa tattaatgca gtcgctatga agattactgc agatattatg caacacgcag
      181 attgaggtta agtcgaagaa cagccaatga attgacttta caagagagat aacttatttg
      241 ttttagaatc atgaatgttc gcccgagcta acctgttaaa ggtgcaaaag attgcacctt
      301 tttcagatta cctcgggcct ggttacgtcg ctttcggctt actgattttc ctcaattagc
      361 aacgcttcaa gcaaatcgag atcgtgcagt aatttttgca gcgtttcgtt gctgatctcc
      421 cgcgtggcgc gcaggtggta cagttcagca cgttcagaac gcaatgccgc cagacggaag
      481 cgacgctcaa ggttctcttc ctgcacggaa ctttcaacgt cgttacgtcc atcggcgcga
      541 cgacgcaggt taccaatgac gcgagaactg acctccgtaa gcaactggtt atcgatattc
      601 tcttcggtat ccgccgccag acgctcctcc attttctgga tcgccacaat cgccacttct
      661 gccgttgccg ctcgcgcaat acgttcttct ttcaattgtt gcgaatggtc cgcgacttca
      721 atgtgttgta gcagaatagg caacatcacc acgccgacaa acagcgagaa gagaatgacg
      781 ccagccgcta ggaacaccag ctcatagcgc gccgggaaga cgttaccatc cggcaagagc
      841 agcgggattg agagcacacc ggccagagtg atcgccccac gcaccccagc gaaagacgcg
      901 atcaggattt ctcgtgtggt ccacgaacca aactccatcg gcttcttctt caggaagcgg
      961 ttgctgaact ttttcatcgt ccacagccag ccgaaacgga ccagcatcag cgccgcatat
     1021 atcagaataa tatcggtaaa cagcatccag atttcgacgt tagggtcgat ttctgccgcc
     1081 atcagcgacg tctccagaat acccggcagt tgcagaccta acagcaggaa caccatgccg
     1141 ttaaagacaa attccagcat cgcccaggtg ctgtttgcgc gcaggcgcat tgccagtggc
     1201 gcacggcgca tcacaccgga gcgggtgatg gtcatcccgg cggcaaccgc agcgaggatg
     1261 cccgaaacgc caatatgttc ggcaatcaga taagaagcga atggcagcaa gaacagcaga
     1321 acgatctgcg tcgcgggttc atcaccgccc cagcggctga ggaatcgcag cgagcgacca
     1381 tacagccagc tcaccacaaa accggcaaga ataccgccaa tggctacttt cataaattcg
     1441 accgtcgcac cgccgacagt gaagatcatc gtccccatcg ccactgccac ggcaaacttc
     1501 aacgacacca ggccggaagc gtcattcatc aacgcttcgc cctgcaaaat gcccatgatt
     1561 tttttcggga tgcgcccttc gccaacaatc ccggagagcg ccacagcatc ggtcggagaa
     1621 agtaccgccg ccagcgcaaa ggcggggatc agcggaatgc ccggcaccac ccagtaaata
     1681 aggaagccaa tgccgaccac ggtgaccacc accagcgcca gcgcgaggcc gaaaatctct
     1741 cgaccatgtt cgagaaattc acgggtcggc gttttccagc catcagcgaa cagcaacggc
     1801 gggataaaca agactaaaaa gagttcagga tcaaactcca catgcaaacc aaacgtcggc
     1861 cacgccagta gcgcaccgat ggcgatttgc ataagcggaa gcgggatctg aaagggcatg
     1921 acacgagtga ccaccccgga cagcgagacc acgagggtca ttatcagtat ggtgaagaag
     1981 atttccatgc gttccctgtt tgcgatgttt tttatgatct gagaaagacg ttatgcctga
     2041 attctcattt tttcagaata tcgcaaaagc tcacacaatg tgcggagaaa ataaaaacgg
     2101 gcagccattg ccacccgttt tcagaatccg atgaaagatt agatagccca gccacccgca
     2161 tagaaggtca ccagcgccac ggcgataacg accgtaccga tgttcaactt gcgccattca
     2221 ccggaaacca gacgaccaat caccagagtc gcgaagccga tcatgatgcc tgttacgatg
     2281 ttacaggtca gcacgatgaa taccgccgta accagacccg ccatcgcatc aacaaaatca
     2341 gcaaagtcga ttttcgccac gttgctcagc atcagcaggc caacgtacat cagcgccgga
     2401 gccgttgcat accccggaac gaggtaagaa agcggagaga ggaacagaat cagcaggaac
     2461 agcacgccaa cggtgatagc cgtcaacccg gttttaccgc ccgccgccgt acccgccgca
     2521 gactcgatgt ataccgctgc cggagctgca cccaccaggc cagagaaaac gctgctcatg
     2581 gagtcagtgg tcagtgcttt cccgccgtcg atgatctgcc catctttatc cagcaggttc
     2641 gcctggccgg cgacagcgcg gatagttccg gtggcatcaa atactgccgt catcaccagc
     2701 gccagaacgc ttggcaggac tacaggattc agcgcgccca taatgtccag gctgccaatc
     2761 agtgaattgc cgttttcatc gctcaatgaa ggcatggcga aaacgccgga gaaatggacg
     2821 ttaggatcga agatcaaacc gacaattgag ataccgataa tggtcagcag aatgccacca
     2881 gggactttca gtttttccaa gccgatgatc accgccagac ctaccagtga cataatcacc
     2941 gggaaggtca cgaaatcacc cagcgcaacg ggcagaccat caagcgggtt tttaatcacc
     3001 agaccgacac cattagcggc aatgagcagc aggaacagac cgataccgat ccccgtgccg
     3061 tgcgccacac cgtgcggcaa gttgcgcaaa atccagctac ggatacccgt ggcagaaatt
     3121 accgtaaaca gcacacccat caggaacacg gcacccagcg cgacaggtac gctaatatgt
     3181 tgccccagca ccaggctgaa tgcggtaaac accgtcaggg agatggcgca accaatcgcc
     3241 aacggcagat tagcccacag acccatcacg atagaaccga gtccggcaac cagacaggtt
     3301 gcaacgaaaa ctgccgcagg cgggaagcct gctttaccca acatacctgg aacgacgatg
     3361 accgagtaga ccatcgccag aaacgttgtt aacccggcaa ccacttcctg acggacagtg
     3421 cttccacgtt gtgaaatttt aaaccaggcg tcgagtgaac cgccggtacg cgctgatggc
     3481 gtagacataa gaaaacatcc cctgagagtt taattttcgt caagatgcgt ggcggacaat
     3541 cccctgccag cgaaacgtta tcttcctgct ccaggtttgc ggcaatttgg tgcgtcgcaa
     3601 aaaagcaaac gtttagctcc gcgcatacaa aacgggtggt aaaaaaaagc aaacgattat
     3661 ccggcgatca tagcggcttt ttcaaccgaa gtttgctgct tttttctctt ttattgtctt
     3721 gacgatgaaa aaccaggcaa ttgatgcgca aacgttatcg tcgatgcgca agattgcagc
     3781 gttatcgagc cgtataccgt tctggattgc gataaaaaat cgtctgagat gacaaaaata
     3841 ttgcggctaa aaagtatact tttacactgg cgaagaaacg tcggtgacat acaattaaag
     3901 catcaacacc aaccggaacc tccaccacgt gctcgaatga ggtgtgttga cgtcggggga
     3961 acccctcctg tgttccagcg ggatagagag aaagacaaag accggaaaac aaactaaagc
     4021 gcccttgtgg cgctttagtt ttgtgtttgc ggataattcc aaaagagctc gatcagtccc
     4081 ctcgcccctt cggggagagg gttagggtga ggggaacagg cagcaccagt gcaaatattt
     4141 accctcaccc cggccccata gttaacgatg agtcagcttg tccttgtggc gctttagttt
     4201 tgttcatctt ccagtaagcg tgcgccggta ccttcttctc ctaagcggtc gcccgggtta
     4261 cgcaacgggc aatcactgcg cgaaaggcag ccacaaccaa tacatccgtc cagttcgtca
     4321 cgcagcgcca ctaaggtatg aatgcgccga tccaactctt ctcgccattg ggacgaaagc
     4381 tgtttccact ctttcgcact taacgtatgc ccttctggca acacgccaaa cgcttcacca
     4441 atggtcgcca gcggaatgcc aatacgctga gcgattttga taattgcgac atatcgcaac
     4501 acatcacgtt tatatcgccg ctgattgccg ctgttacgga tactagtaat caaccctttg
     4561 ctttcataga aatgcagcgc cgataccgcc acaccgctgc gtttcgccac ttcgccaggg
     4621 gttagcagcg ctttaatgcg gggtaatttc ttttccataa atcgctttac ctcaagttaa
     4681 cttgaggaat tatactcccc aacagatgaa ttaacgaact gaacactgaa aagaggcaga
     4741 tttatgtccc atcagaaaat tattcaggat cttatcgcat ggattgacgt gcatattgac
     4801 cagccgctta acattgatgt cgtcgcaaaa aaatcaggct attcaaagtg gtacttgcaa
     4861 cggatgttcc gcacggtgac gcatcagacg cttggcgatt acattcgcca acgccgcctg
     4921 ttactggccg ccgttgagtt gcgcaccacc gagcgtccga tttttgatat cgcaatggac
     4981 ctgggttatg tctcgcagca gaccttctcc cgcgttttcc gtcggcagtt tgatcgcact
     5041 cccagcgatt atcgccaccg cctgtagttt tattgcccgc gcgttaactc ccgcgcgggt
     5101 aattgctcca tccattgcat aaacacctgc ggcggcatcg ccttcgcaaa aaaccatccc
     5161 tggcaatagc gcacgccgcg tttgcgcagc cagttaacct gctcctcagt ttcgacgcct
     5221 tcagcgatcg tttttaaccc caggctgtgc gccagctcga tgatgtgttc cgcaatcaaa
     5281 tgactggttt tgtgggtggt cagcgtttca acaaacgatt tatcgatttt caaaatatcg
     5341 acattcaatg atttaaggtt atgcaagtta gagtagccaa taccaaaatc atcaattgcc
     5401 acttcgtaac cagcctggcg gaaagccaga ataatcggcg tcatt
//
LOCUS       DADLSA010000212         5353 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.213, whole genome shotgun sequence.
ACCESSION   DADLSA010000212 DADLSA010000000
VERSION     DADLSA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.213"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            253..510
                     /locus_tag="JN691_003885"
                     /pseudo
     CDS             253..510
                     /locus_tag="JN691_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(560..1510)
                     /gene="uspE"
                     /locus_tag="JN691_003886"
     CDS             complement(560..1510)
                     /gene="uspE"
                     /locus_tag="JN691_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAY7245171.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1662..2414)
                     /locus_tag="JN691_003887"
     CDS             complement(1662..2414)
                     /locus_tag="JN691_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HAY7245172.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2609..3124)
                     /gene="ogt"
                     /locus_tag="JN691_003888"
     CDS             complement(2609..3124)
                     /gene="ogt"
                     /locus_tag="JN691_003888"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAY7245173.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3135..3800)
                     /locus_tag="JN691_003889"
                     /pseudo
     CDS             complement(3135..3800)
                     /locus_tag="JN691_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            3897..4925
                     /locus_tag="JN691_003890"
     CDS             3897..4925
                     /locus_tag="JN691_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7245174.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKLRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKLGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            4922..>5353
                     /locus_tag="JN691_003891"
     CDS             4922..>5353
                     /locus_tag="JN691_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAY7245175.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLS"
BASE COUNT         1380 a         1206 c         1302 g         1465 t
ORIGIN      
        1 agcgatcatt gttttatcag gctcctccag ataattgtcg tcatgccgga aacccctggc
       61 ggggctattt taccgcgaca attcattcag atcatcaata gtcagggaag gaagtagcaa
      121 cattagctaa ctaatcaatg cgttgattgt aaatccagct aagaggtgag gttttcagag
      181 cagacaacgg tgaaacgtca tggtattgtt acgtttaggt aacaagaaat ttgtctgcac
      241 aaggattaca tcatgattat ggcgaaactg aagtcagcga aagggaagaa atttctcttt
      301 ggtttgttgg cggttttcat tattgcggcg tcggttgtga ctcgcgcaac catcggcggc
      361 gttatagaac agtacaatat tccgctgtct gagtggacga catcaatgta tgtgatttag
      421 tcatcgatga tttttgttta tagcctggtc tttactgtgt tgctggcaat cccgttggga
      481 atttatttcc ttggcggcga agagcagtaa gtaaaaaata ggcccgataa ctcgggcctt
      541 gtcagttatt gaagagtcgt taatcgtctt cttcgtcatc cagttcaacg ggtgtctgat
      601 actggtcagg tttaatgacc agcaggtcgc agcgaagatg atcaatcacc tgttccgccg
      661 tgttgccgag gaatgctgct gaaataccgg tgcgtccgac cgtgcccaga accacaattc
      721 ccgcctgtaa atgctccgcc aaatcaggaa tcacctcttc gggcagacct ttttctacgt
      781 gcgtcatgtt ttcattaatg ccgaatttct gccgcagggc tttcattgcc agcaaatgtt
      841 gcccacgaat ggcatcgtta taaacgctcg ggtcaaattc cggcagttca atcgcgatat
      901 taattggcgt taccggataa gcgccaacca gatgaacttc ggtatggttg acttgttctg
      961 ccagttcgat cgtctctttg accagttttt cattgagcgc attatgatac ggctcttcac
     1021 tggcgagatt caccgccacc agcgccttgc ctccttccgg ccacggctgg tctttcacca
     1081 tccacaccgg gcttgggcat ttgcgtaaca gatgccagtc cgttggcgta aaaatcaccg
     1141 cttccagacg gtcatgttgg tgcgccattt ttagcaccaa atcgtgtccg ccgctgatca
     1201 cttcctgaat gatggcttcg aaaggacggt tatgccagac cactttaatt tcaatgggaa
     1261 cgccagcatt gagataatat tttgcctgct cgtggatcca ggctgtacgc tggctgatga
     1321 cgccctgacg catagcggta cgttcgtccg gggagagcag ggtggtcatt tcgtatgaga
     1381 agtcatagat cggcaaaaag gctttaattt tgccaccaat ccgttgatgt aaataaacag
     1441 ctcgccgcaa tgctggttgg tcgtcctggt taggatcgat aacaacgagc atgttctgat
     1501 acatagccat acagggtctc cttacaacaa ctgtcaacgc agtttgtaat taaaagatta
     1561 acccatatct ggtgaatgaa acagtgatga accttctgcc agatcaataa atcagaaaaa
     1621 tttaatgata tgacagaagg atagtgagtt atgcggaaaa atcaggcaac gttacgcgta
     1681 tgaccagcaa gctgggccag cgcatcgtta ttttcgatgg tgatgtattt acctttgact
     1741 gccagcatac cgcttttctg gaagcgaccc agcagacggc tgatggtttc taccgtcagg
     1801 cccagatagt taccaatatc gccacgagtc atcgtcaggc ggaattcacg aggggagaag
     1861 ccgcgttggg caaaacgacg ggacaggttg tagatgaatg cagccagacg ttcctcggca
     1921 tttttcttcg acaacagcaa gatcatgtcc tgatcgcctt tgatttcacc gctcatcaga
     1981 cgcatcatct gctgacgcag attcggcatt ttaccggaca aatcgtccag cgtttcgaac
     2041 ggaatttcac ataccatcga ggtttccagc gcctgagcga agctcgggtg atggccgctg
     2101 ccgatggcgt caaatcccac taggtcgcct gctaaatgga aaccagtgat ttgctcgtcg
     2161 ccttgctcag taattgtata acttttaatc gtaccggagc ggatggcata aagcgattta
     2221 agttcatcac cagccttaaa cagcgtctgg cctttctgaa taggcttctt ccgctcaatg
     2281 atattatcaa gctgatcaag ctcatgttcg ttgagtgtga acgggatgca aagctggctg
     2341 atgctgcaat cctggcaatg gatagcacaa ccgccagact gaatgcgccg tataattcgc
     2401 ttttccggga tcataggtct gctcaagccg taattgatat ttgtcaattt taacatcttt
     2461 ttagggagca agtaagtcta agcaaacctt aacagcagag aattccgata ttagatgtaa
     2521 atatatgtct atctatttga aactccttaa gttgtcaagg gtaactttac ataaaagtgt
     2581 gaacaagctg gcacaaattg tttaatgttt acagcaaaag ataaccttcg tggcgcaata
     2641 accactcttt tcgctgaact ccgcctgcat atccggtcat ggtgccgttt cggccaataa
     2701 cccgatggca aggtacgacg atgctgatgg gattcgatcc gtttgccgca ccaacggcac
     2761 gcgccgcgcc agggcggccc aattgttcag ccagttggcc gtaatgcatt acctgcccgc
     2821 aggggatagt gcgtagtgtt ttccagactt cgcgctgaaa tggcgtcccc cccgtggcag
     2881 tgggaagcgt atcaataatg ctaagattac cggcaaaata ttcacgaagc ttgtcgctta
     2941 aaccacctgg gttggtggca gaaatgcgct catagccttc tttgcgataa tggatgtcca
     3001 gcagctgcac catgcgttcg ctgtactctt cccattcaac cgcccgcagg cgaaattgct
     3061 catcgcaaat cacccacagt ggacccagtg gcgtggcaat tttttcttca agtaatctca
     3121 gcatccgttc tctctccatc atcaagaagc gtcactgcag aaataacgtg catttgctga
     3181 acccagtggc gaaatgttaa tcccgtctct ttaacaaata agcgcatgag tgtacggcta
     3241 ctgacgttaa ttttatccgc tagattatcc agtgttggca gatacccttg gttgtttagc
     3301 aaattctcac aaagctgctg tagccgctca tcatttggcc aagggagttc aagtgacatt
     3361 ttcaccccca tttttatttc ctgaagtatc agtgtctcga gggctgaaaa acgtaactgt
     3421 tgtttatctg tggtactttg cgacggtgga agttggttaa ttgcaattag taattccctt
     3481 aacaggggtg aaatacccgt catgaagcaa actgttccca gcgtttcact ttccgcttct
     3541 gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa caaacttatg cggcatttgt
     3601 gggggcagcc aaagcgcgct gctggcatgg actagccaga cgctttctcc agcccataca
     3661 cgaacgactc cttcagccgg gtaaagcaac tgggcataga gatgtttatg ccagggaact
     3721 gtacctccat tcagatgttc cgaatccagt ccgataatgt gatgccctgg tgatgctttg
     3781 ggttgcagtg taattgtcat ttacagtagg tattttgtca gaaatcatca aataagccat
     3841 gttaaatttt aaatatgacg tatgcaagtt tccggatata tacggtgtaa agagtgatga
     3901 taatttctaa aaaacagctt attggggtgg tagcgatcgg tattcttttg actggcgttg
     3961 tcttttttat ctggagggta agcaaaggaa gatttataca gacgacggat gatgcttaca
     4021 ttggtggtaa tatcaccacg gtggcaagta aagtttctgg ttatatttct gctatagagg
     4081 tgcgcgataa tcagagcgta aaaaaaggcg atattatcct gcgtctcgat gatcgggatt
     4141 atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag tagcaaagcc aatctcgaag
     4201 gcattcaagc cactattacg atgcagcagt cgataattca aagtgccagt gaaacatggc
     4261 aggcagtaaa acatgaggag caaaagcgcc tgcgagatac tgaacgttat gaaaaactcg
     4321 cacaatcagc ggcaatatcg caacaaatta ttgataacgc caggtttgac taccagcagg
     4381 tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt ggagaagcta cggctggcag
     4441 ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga agaagttcag gccgctttga
     4501 ctcaggcact actggatctg gaatataccc ttgttcgtgc gccgattgat ggcattgtcg
     4561 caaaccgcag cgcccataca ggcagttggg tggaaggcgg cacatcgtta gtttcactag
     4621 taccagtcag tgagttgtgg gttgatgcaa attataaaga aaaccaaata gcgggaatga
     4681 agctaggaat gaaggctgag atccgtgctg atattctcaa aggggaagtg tttcacggtc
     4741 acattgaaag cctttcccca gcgacaggag ccagtttcag cttgattccg atagaaaatg
     4801 ccactggcaa tttcaccaaa attgttcagc gtgtcccggt acggattgca tttgacgacg
     4861 caaaggaatt aaagcaactt cttcgcccgg gactgtccgt caccgtcagt gttgatgaga
     4921 gatgatgtct atgcgtaaac atattgcttt cgccagtatg tgtatgggat tatttattgc
     4981 tcagcttgat attcaaattg tatcgtcatc cctaaatgaa ataggtggtg gtctgtccgc
     5041 aggtaaagac gaaatggcct ggcttcagac cagctattta attgctgaga ttattgttat
     5101 tccgctctct gggtggctgt cacgggtatt ttccactcgt tggcttttta ctctatccgc
     5161 gggaatattt actttaatga gtatcgcgtg tggacttgca tggaatattc agatcatgat
     5221 tttgtttcgt gctttacagg gagctgctgg cgcatccatg atccctttag ttttcacgac
     5281 ggcgtttatt tattaccagg gtaaagagtt ggggttggct gctgcggtag tcagtgcgct
     5341 ggcctcactg tca
//
LOCUS       DADLSA010000213         5333 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.214, whole genome shotgun sequence.
ACCESSION   DADLSA010000213 DADLSA010000000
VERSION     DADLSA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.214"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..232
                     /locus_tag="JN691_003892"
     CDS             <1..232
                     /locus_tag="JN691_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HAY7245176.1"
                     /translation="VVEKEQVESWMANQLAQLEKCGPVALAHIKQLILALENKHADYP
                     HQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            242..1573
                     /locus_tag="JN691_003893"
     CDS             242..1573
                     /locus_tag="JN691_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HAY7245177.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGSGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGLHNGMMLDDAFFGCARNADA
                     IIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGGFLEIAQNARKVV
                     FCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQSGQEVLYITERA
                     VFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFTPMEDA"
     gene            1570..3642
                     /locus_tag="JN691_003894"
     CDS             1570..3642
                     /locus_tag="JN691_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAY7245178.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSKLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQHGRRVFMDFNRNPEAVPGDLPFTLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNSGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVIAVRLARFIGCTQKRSVEGDVAQLVASS
                     LNIVREVIAQALVNENGMALVTVKEKIQARMSDYAGFICHADKVRHATRDALLLSEFV
                     QQHGLTINHAGEITELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIVIDPQGKCLP
                     QTRRGAKEEWRFRSERAEDKNHRLTIQYSQGSFITEVKSLRMQPCINGIYFEKNWPDF
                     LNGEIYTQ"
     gene            3735..5012
                     /locus_tag="JN691_003895"
     CDS             3735..5012
                     /locus_tag="JN691_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7245179.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPVMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWTLIKVKDEEPQVQLATH"
BASE COUNT         1326 a         1260 c         1377 g         1370 t
ORIGIN      
        1 agtggtagaa aaagaacagg tggaaagctg gatggcgaac cagttagcgc agctggaaaa
       61 atgcggtccg gtggcactgg cgcatattaa gcaactgatt ctggcactgg aaaataaaca
      121 tgccgattac ccgcaccagc tactggccgg attaatgtct gccactcagg attgccagca
      181 ggcgacgcgc gcctttgccg aaaaaagtag cgtgagcttc cataatcaat aaggaaattg
      241 tatgcgtaag ataacaaccg ccgaggcact ggcggcgcaa attcaggacg gtgcgactat
      301 tgctattagc ggtaacggcg gcggtatggt agaagccgac catattctgg ctgctattga
      361 agcgcgtttc ctgcaaaccg gacaccctcg cgacctgacg ctaatccact cgctgggtat
      421 tggcgatcgc gactgcaaag gcactaaccg ttttgcgcac gcagagatgc tcaaacgcat
      481 tattgccggg cactttacct ggtcgcccaa aatgcaggcg ctggtaaaaa ataacacgat
      541 tgaagcctac tgctttcctg gtggcgtgat tcaggcactg ctacgggaga tcggctccgg
      601 acgtccgggg ctttttaccc acgttgggct gggatcgttt gttgatccac gcaatggcgg
      661 cggtaagtcg aatgaatgca ctaccgacga cctggtagaa ctgattgaaa tcgacggtga
      721 aaccaaactt cgttatcgcc ctttcaaagt ggattacgcg attttgcgtg gcacttatgc
      781 cgatcctcga ggcaacgtca gccttgaaga agaagcgatt gatatggata gctattccat
      841 ggcgctggca gcacacaaca gcggcggcaa agtgttcgta caggtacgcg atgtgctgga
      901 agctggcgct tcaaccaact ttggctttgg tattcctggg ggtattccag gcgtagcgct
      961 gcgcgaaggc gtgccttacc aaagtttatg gctgagcgtc gagcaaggcc tgcataacgg
     1021 tatgatgctg gatgatgcgt ttttcggctg cgcccgcaac gccgatgcca ttattccatc
     1081 actcgatcaa tttgagttct acagtggcgg agggatcgat atcaccttcc tcggcatggg
     1141 agagatggat cagtacggta acgtcaacgt atcccatctc aatggcaatc tcattggtcc
     1201 cggcggtttt ctcgaaattg cgcaaaacgc ccgtaaagtg gtgttctgcg gcacgttcga
     1261 cgccaaaggt agcaagattg atgtaacgcc agatggcttg catatcaccc agtcaggtca
     1321 aatccctaaa ctggttaccc aggtggaaaa aatcactttt agcgccgcct acgcacagca
     1381 aagtggtcag gaagtgttgt atatcactga acgcgcagta ttccagttaa cggcagaagg
     1441 cgttgaatta attgaaatcg cgccgggtgt ggagattgag cgcgacattc tgccgtatat
     1501 ggctttccgt ccaattatca agcacccacg cctgatggaa agtagcctgt ttacgccgat
     1561 ggaggatgca tgagtcagct tgatgacacc atccttgatg cgctgacgca cgttactttc
     1621 ccaaagggtt ttgcacaggc agagcccgca tgggttgtca cggtagacgg tgttgattac
     1681 ccactctggc aaacagatgc cctggtagtc ggcagtggtg cagccgggct gcgtgcagct
     1741 gtggaactta aacgccgcca gcaaaatgtg ctgatcgcca ccgccgggtt atatatgggg
     1801 acgtcggcat gttctggttc cgataaacag acactgttta ccgccgctac cgcgggcaac
     1861 ggcgacaact tcaccaaact ggcagaggca ctggcgagcg gtggggcgat ggatcacgac
     1921 accgcttatg tcgaagcggt aggttctctg cacactcttg gcgggttgca atatcttggt
     1981 ctggaattac cggaagatcg ctatggcgcg attcttcgtt atcaaaccga ccatgacgaa
     2041 gccgggcgtg caacctcgtg tgggccgcgt acctcaaagt taatggtgaa agtgctgttg
     2101 gaagaagtac agcgcctcgc cattccagtg ttgaccagtg caacagtgat taaactgctg
     2161 catcagcgtg acgaaaacgg cgaagaccgt gtggcggggg caatcctcgc gaccggtcat
     2221 cgcgcccata acccttgggg gctggcaatt gtgactgcgc ccaatgtggt actggcaacg
     2281 ggcggaccgg gcgagcttta tcgcgacagt gtttatccac ataaatgctt cggctcgctg
     2341 gggctggcgc tggaggaagg tctgacgcta accaatctga ccgaaagcca gtttggtata
     2401 ggcacaccgc gcagcacgtt tccgtggaat ttgtccggca cctatgtgca ggtgatcccg
     2461 tatatctatt ccgtggatgc tgagggtaac gagtataact tcctcgcgga ttactatcgc
     2521 accacccaag agctggcttc aaacatcttc cgtaaaggct atcagtggcc gttccacgcc
     2581 acgcgggtaa tggattttgg ctccagcttg ttagatatgg cggtggcgca agagcagcaa
     2641 catggtcgtc gggtgtttat ggatttcaat cgcaatcctg aagcggtgcc tggtgatttg
     2701 cccttcacat tagatcgact ggacgacgac gttcgcgcgt atctggaaaa taatgacgct
     2761 ctggcaccat cgcccattga acggctgcaa cgaatgaatc cgctgtctat ctcgctgtat
     2821 aagatgcacg gttacgatct caccacgcag ccattgcagt ttgccatgaa taatcagcac
     2881 atgaatagcg gcattgaagt ggatatctgg ggacaaacat ccctgcccgg ttgttttgcg
     2941 gtgggagaag tagccggaac tcacggcgtt acccgaccag gcggtgcggc gctgaatgcc
     3001 ggacaggtta ttgccgtgcg tctggcgcgc tttattggtt gcacacaaaa gcggtctgtt
     3061 gagggtgatg tcgcgcagct ggtagcctca tctctgaata tagtcagaga agttatcgct
     3121 caggcactgg taaatgagaa cggaatggcg ctggtaacgg tgaaagaaaa aattcaggcg
     3181 cgaatgtctg attacgccgg atttatttgt catgccgata aagttcgtca cgccacccgt
     3241 gacgccctgc tgctaagtga gtttgtacaa caacacggtt taaccatcaa tcacgcaggc
     3301 gaaattaccg aactatttat gtggcggcat atggcgctga cttctgccgc cgtattaacg
     3361 caattgacgc attatattga cgcaggtggt ggcagtcgtg gggcacggat agttattgat
     3421 ccacaaggca aatgcctacc acaaactcgt cgcggcgcaa aagaagaatg gcgttttcgc
     3481 tctgaacgtg ctgaagacaa aaatcacaga ttaacgattc aatattcgca aggttctttt
     3541 attaccgaag tgaagtcttt acgtatgcaa ccgtgtatta acggtattta ctttgaaaaa
     3601 aactggccag actttttaaa tggagaaatt tacacacaat aattttcaat atttcctctg
     3661 tacctgattt ttattaccta ttacacggag acaccaacaa ccagggttta ttaatataat
     3721 taaaaggttt aaacatgaat aaaataaaga attaccggtg gcatatgatt gccctcgtat
     3781 gctttatcac tgtaatcaat tatctggaca gaacggcgtt aggtattgcg gctccaacga
     3841 ttatggagac aaccggaata actaaagagc aatattcatg gattgtcagt gcattccagt
     3901 tggcctatac attagggcaa ccggtaatgg gcttctttat tgataccgtg ggtctgaagt
     3961 taagttttgc gatatgtgcc gcaatttggg gcctggcgac aatgggccat gcactaaccg
     4021 gaacgtggtc tggtctggca tttatgcgcg ccctgatggg tttcagcgaa gcgtcggcaa
     4081 ttccggcggg tgtaaaaacc gcatcaacat ggttcccggc aaaagagcgt ggcgtggcga
     4141 caggtgtttt caatatgggc acctcactcg gcgcgatgct tgctccaccg ttgattgcct
     4201 ggtgcattat gtttcatagc tggcaatttg cgtttattgt ttcaggtagc cttgctttgc
     4261 tcgcggcttt attttggttc ttttgttata aagatccgaa agatgccaaa cgcctttctg
     4321 atgaagagcg ccactatatt gaatcaggac aagaacagca tcttaaaaca gataagaaag
     4381 aaaaaacgtc aatcaagcat atcctcagcc aacgtaattt ctgggggatt ggcatcgcgc
     4441 gttttctcgc agaccctgca tggggaacca ttaacttctg ggtgccgatt ttcttcgtcg
     4501 aaacgctgca ttttagcctg aaagaaattg ccatgttcgt ctggctgcct ttcctgctgg
     4561 gcgatctcgg ctgtttagcc agtggttttg tcgcgaagtt cttccacgat cgcggcgtga
     4621 gtttaattaa ctcacgaaga attaccttca ctattgcagc cgtcattatg atgacgattg
     4681 gcctggtgag tattgtcgaa aatccctaca ttgccgtatt actgattagt attggcgcgt
     4741 tctcgcatca atgtctttct actgtagcag caactctggg tggcgatctg ttcaaaaaag
     4801 acgaagtagc taccgcagtg ggtatggcag gagcctgtgc gtggagcggt cagttgattt
     4861 tcaacctgtt catcggggca ttcgttcaca ttatcggctt cgcgccgttc tttattgccc
     4921 tggctttctt tgacattatt ggtgctattg cgttgtggac gcttatcaaa gttaaagatg
     4981 aagaaccaca agtacagtta gctacccact aattatttta aataaaaacg ctataattga
     5041 ggttatggcg tttttaaaga agcttgagtt atcgaacctc gcttaccaac gcgttgtcag
     5101 tcatctcccc cctccctggt gcatcgttat atccgtgatg agcgattttt gatattaata
     5161 tctttcacca tccgctattt tcaccacttc agaaaacaaa cttattttta ttaaaatcaa
     5221 ttctttttct ttgaacattc aactgcgata tttgtaaaaa cattaaacag gcaacaataa
     5281 aatgacatta ccaacaacta tttattcatt ccccgcatat ttaccccgtt ttt
//
LOCUS       DADLSA010000214         5327 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.215, whole genome shotgun sequence.
ACCESSION   DADLSA010000214 DADLSA010000000
VERSION     DADLSA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.215"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(228..303)
                     /locus_tag="JN691_003896"
     tRNA            complement(228..303)
                     /locus_tag="JN691_003896"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(268..270),aa:Phe,seq:gaa)
     gene            complement(409..1116)
                     /locus_tag="JN691_003897"
     CDS             complement(409..1116)
                     /locus_tag="JN691_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAY7245180.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLDALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1514..3649
                     /locus_tag="JN691_003898"
     CDS             1514..3649
                     /locus_tag="JN691_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY7245181.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACSGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFVPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3698..4954)
                     /locus_tag="JN691_003899"
     CDS             complement(3698..4954)
                     /locus_tag="JN691_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAY7245182.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5156..>5327)
                     /gene="mltC"
                     /locus_tag="JN691_003900"
     CDS             complement(5156..>5327)
                     /gene="mltC"
                     /locus_tag="JN691_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708734.1"
                     /note="Murein hydrolase C; membrane-bound; lytic;
                     catalyzes the cleavage of the beta-1,4-glycosidic bond
                     between N-acetylmuramic acid and N-acetylglucosamine
                     residues; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY7245183.1"
                     /translation="GSVLRVFSNDKIQAANIINTMTPGDVYQTLTTRHPSAESRRYLY
                     KVNTAQKSYRRR"
BASE COUNT         1332 a         1278 c         1407 g         1310 t
ORIGIN      
        1 ccgtgcagta atgaatccgt tagccaggtg ctcatgtgga aatagtgtct attggggatt
       61 tgaagtttga cagaaaaggc cacagtgacc agaagggggc tacttagaag aaatgttcag
      121 gctggatgga agaggtaaag cagtgacatc agcattcaaa gtgaccggat atctaccaga
      181 accccagaaa gcaaaaaacc agcccgtagg ctggttcttc taaatagtgg tgcccggact
      241 cggaatcgaa ccaaggacac ggggattttc aatcccctgc tctaccgact gagctatccg
      301 ggcaacgggg cgcattaaac ctgattcgcc tcgtctcgtc aatcaaattt cttcaattta
      361 ctgcagactg cacaatctat catcactttg ccgctattgc acgcattgtc aggcaaacca
      421 ggcggtccag gcggcggaaa gcggcattgc cagaaggagt gctggaagca tgttgaccac
      481 cgggaacatt ttaatgccac agatgcgtaa tccggttgcc agcagcaata aaccgcctac
      541 ggcgctgaag tctgccatca tcgacggtgt ggtcagcggt aatatcagcg cggcagccca
      601 cgccagcgtt aactggatga tcagtaatgg gatactaatt accgacaccg caatacccag
      661 cgagcaggcg aagatcatcg ccgtaaagaa atcaagaaat gacttggcga ttaaaatact
      721 cggatcgccg gtcatccctt cgttcatcgc cccgaagatc ccggtgccgc tggcgcaaaa
      781 caggacaata atcgcgacat aattctgaat aaaagattca tgcgctggct tcttacgtga
      841 gtgacgaaac agattttgtg ctttggcgac tgctgtattg acacctttct ccagcaaaca
      901 aatttcgcca attagagcgt caagtagcgt agctaaaacc atcgctggaa ggttggcgca
      961 tttcacaacc agtaaaatac caatccccag cgatgccaga ccaaaaatag atgtcatgga
     1021 gatgcggata cgttccggta agcgttggct gagcagtgcg ccgagaacgc cacccagtaa
     1081 gactgcacta gcgttgataa aagggccgat gaccacaaga gttcctgtta gctgtattgt
     1141 ttgattttct tattatgaac tttttgcctt gttgatggct tgtctggtcg caaagtcctg
     1201 gcttgcacgc cttagcgaaa ggtgccatga ttgcgcgaat tttctcctct ctgtacggag
     1261 tttgcccgat gcacgccatc tccttacatt ctctcgctta tcgccgtttc gcgcgaaacg
     1321 tttccctttc tatgttactg ctcatgcggt gaagttaacg cacgctcact gcaggacaac
     1381 agtaaaatca gagcgtttct gcttttactg atgtctggcg gtcggagctg gtgaccagtt
     1441 tgacccatat ctcatggggc agggttttcc accttgtccg gtattcttac ttccccgaaa
     1501 cgggtttgcg cttatgaaat caatgaatat tgccgccagt agtgaactgg tatcccgact
     1561 ttcttctcat cgccgcgtgg tggcgttggg agatactgat tttacggacg tcgcggcagt
     1621 cgtcattacc gctgcggata gtcgcagtgg cattcttgcg ttgcttaagc gcaccggttt
     1681 tcatctaccg gtgtttttgt attccgaaca tgctgttgaa ttacctgcgg gcgttacggc
     1741 ggtaatcaac ggcaacgagc agcagtggct ggagctggaa tccgcagcct gtcagtatga
     1801 agagaatttg ctgccaccgt tttatgacac gctgacgcag tacgttgaga tgggcaacag
     1861 cacctttgct tgctctggac atcaacatgg tgcgttcttt aaaaagcatc ctgccggacg
     1921 ccatttttac gatttctttg gtgagaacgt ctttcgcgcc gatatgtgta acgctgacgt
     1981 aaaattgggc gatctgctta ttcatgaagg atcggcgaaa gatgcgcaga aattcgcagc
     2041 caaagtcttt catgccgata aaacctattt tgtgctgaac ggcacatcgg cagcgaataa
     2101 agtggtgacg aatgcgctgt taacgcgtgg cgatctggtg ctcttcgacc gtaacaacca
     2161 taagtcgaat catcacggcg cgctgattca ggcggggacg acgccggtct atctggaagc
     2221 ttcacgtaac ccgtttggtt tcattggcgg tattgatgcg cactgtttta atgaagagta
     2281 tctgcgccag caaattcgcg acgttgcgcc agaaaaagcc gacctgccgc gcccgtttcg
     2341 cctgacgatt attcagctgg gaacctatga cggcactgtc tataacgccc gtcaggtgat
     2401 cgataccatt gggcatctgt gtgattacat tctgtttgat tccgcgtggg tcggttacga
     2461 acagtttatc ccgatgatgg cggatagctc gccgctgctg ttagaactta acgaaaacga
     2521 tccggggatt tttgtgaccc agttggtgca caaacagcag gctggattct cacagacgtc
     2581 gcagatccat aaaaaagata accatatccg cggacaggcg cgtttttgcc cgcataagcg
     2641 gttgaataac gcctttatgc tccatgcttc taccagccct ttctatccgc tgtttgctgc
     2701 actggatgtt aacgccaaaa ttcatgaagg ggagagtggg cgtcggctgt gggctgagtg
     2761 cgttgagttg ggtattgagt cgcgcaaggc tattcttgcg cgctgtaagc tgttccgccc
     2821 gtttatcccg cccgttgttg atggcaaatt gtggcaggat tatccgacat cagtgttagc
     2881 cagcgaccgc cgttttttca gttttgtgcc gggggcgaag tggcacggct ttgaaggata
     2941 tgccgcggat cagtattttg ttgatccgtg caagctgtta ctcactacac caggtatcaa
     3001 tgccgaaacc ggcgaatata gcgactttgg cgttccggcg acgattctgg cgcactatct
     3061 gcgtgagaac ggcattgtgc cggagaagtg cgatctcaac tccattctgt tcttattaac
     3121 tccggcggaa agccacgaga agctggcaca actggtggcg atgctggcgc aatttgaaca
     3181 gcatattgag gatgactcgc cgctggctga ggtgttgccg agcgtttata acaaatatcc
     3241 ggtgcgctat cgcgactaca ccctgcgcca gttgtgtcag gagatgcacg atctgtatgt
     3301 cagtttcgac gtcaaagacc tacaaaaagc gatgttccgc cagcagagtt tcccgtcagt
     3361 ggtgatgaac ccccaggatg cgcatagcgc ttatattcgc ggtgaagtgg agttggtgcg
     3421 gattcgtgat gccgaagggc gaattgcggc agaaggggcg ttgccttatc cacctggcgt
     3481 gctttgcgtg gtacccgggg aagtctgggg tggggcggtt caacgttatt tccttgcgct
     3541 ggaagaaggc gtgaatttgt tgccgggatt ttcgccggag ttgcaaggtg tctatagcga
     3601 aaccgatgcg gatggcatga aacgattgta cggttatgtg ttgaagtaag aataaaaaaa
     3661 cgggtcacct tctggcgacc cgtttttctt tgcgtaatta gtggctaacc gtctgtgtgc
     3721 ctgtcgggac acgaacgtgt ttatatttga acatcgccat gaacgcgaag gccagaacca
     3781 cggaataacc agcgaaaatc aaccatacgg tctgccagtc ggtaatgccg ttttgggtgt
     3841 acatctcaac aactttaccg ctcacgatgc cgccgaggat acagccgaag ccgttagtca
     3901 tcatcaggaa catcccttgt gcactggcgc gaattgccgg gctaacttct ttttcgacaa
     3961 acaccgaacc agagatgttg aagaagtcga atgcgcaacc gtaaacgatc atcgacagta
     4021 ccagcagtac agtaccgaac ggagtcgggt cgccgtaagc aaacagcgca aaacgcagga
     4081 tccacgccac aatactgatc atcattacgt tcttaatacc gtagcggctt aagaagaacg
     4141 ggatggtcag aatgaacagg gtttcagaga tctgcgaaat cgacatgatg attgacgcat
     4201 gctgcacgat gaagctgctg gcaaacatcg gatctttgtc gaagctgtgc aggaaggtat
     4261 taccgaacat gttggtaatc tgcagttccg cgcccagcag cattgagaag atgaagaaga
     4321 ttgccatacg cttgttttta aacagcgcga atgcatcgag gcccagcagg gttgtccagc
     4381 tctgattcgc ttgctgttta gcaaccggaa tatgcggcag agtcagggta aacagaacca
     4441 gaatggcgga aagtgctgcg ccaatataca gctgcatgtg gcttaattcg aagccagaca
     4501 ggctcaccac ccacattgcc atgataaagc cgatggtgcc ccagatacgg attggcggga
     4561 agtcagtaac gatatccatc ccggcatttt gcaggcgata gtaagagatg gtgttgatta
     4621 acccaagcgt tggcatataa gcaaacgagt taatcaatat cacaaggaac atcgcttccg
     4681 gcgtcgtgac ctgtgccgcc atgaacagcg tgatagcgcc aatggtgtgg caaatggcat
     4741 atacccattt cgcacttaac catttgtcgg ccacaatccc cagcagcgca ggcataaata
     4801 ccgcggcgat acccagtgag ctataaactg cgccaataga agcaccgtca aacttcaggg
     4861 taacaaacat ataggagccg agggtcgtca gccaacttcc ccacagacag aactgcagaa
     4921 aagagaggat tttcagctgc agcttaagat tcatgttaat ttcctcacat cgtgatgcgg
     4981 atgagtgttt gaaaaagcac atttgaccgt gcgagatgat gggattctat caacttcgcg
     5041 gactggtttt ttgttacctg tggcaaataa ttgcaaacat tcccaatttg caaaacgaaa
     5101 ttgtgatgtg gataacattt ttgcccctga gcatcgtcag gggcggttaa tggaattatc
     5161 ggcggcggta ggatttttgc gcggtattca ctttataaag ataacggcga gattccgcag
     5221 agggatggcg ggtcgtcagc gtctgataaa catcgcccgg cgtcatggtg ttaataatat
     5281 tggcagcctg aatcttatca ttcgaaaaga ctcgcagcac gctgcct
//
LOCUS       DADLSA010000215         5309 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.216, whole genome shotgun sequence.
ACCESSION   DADLSA010000215 DADLSA010000000
VERSION     DADLSA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.216"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..1808)
                     /locus_tag="JN691_003901"
     CDS             complement(<1..1808)
                     /locus_tag="JN691_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7245184.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSAGDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGTLRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARS
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGN"
     gene            complement(1856..2065)
                     /locus_tag="JN691_003902"
     CDS             complement(1856..2065)
                     /locus_tag="JN691_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HAY7245185.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            complement(2161..2535)
                     /locus_tag="JN691_003903"
     CDS             complement(2161..2535)
                     /locus_tag="JN691_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7245186.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(2541..3257)
                     /locus_tag="JN691_003904"
     CDS             complement(2541..3257)
                     /locus_tag="JN691_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7245187.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFVKNLEKTLTVN
                     TGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSA
                     EQPQSITSDACTVTVNGAGG"
     gene            complement(3324..3668)
                     /locus_tag="JN691_003905"
     CDS             complement(3324..3668)
                     /locus_tag="JN691_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HAY7245188.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(3665..4111)
                     /locus_tag="JN691_003906"
     CDS             complement(3665..4111)
                     /locus_tag="JN691_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAY7245189.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(4108..4458)
                     /locus_tag="JN691_003907"
     CDS             complement(4108..4458)
                     /locus_tag="JN691_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY7245190.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(4468..4794)
                     /locus_tag="JN691_003908"
     CDS             complement(4468..4794)
                     /locus_tag="JN691_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY7245191.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(4791..>5309)
                     /locus_tag="JN691_003909"
     CDS             complement(4791..>5309)
                     /locus_tag="JN691_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY7245192.1"
                     /translation="PVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAID
                     DQKSFPRGTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSS
                     GQRTEKTFSLLVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTADKPGDAHASGWTL
                     AAKRGRDAGGGK"
BASE COUNT         1110 a         1773 c         1276 g         1150 t
ORIGIN      
        1 tcattaccca gccccagcag acggttgaat tccgtatgct gaccgttcat aaccagcatg
       61 gactggtaca ccttattctg ctctgccgcc tgctgacgaa ttaacgccac acgacggtct
      121 tccagcccgg caagcacatc ctgaatggac tgcgcttttt cctgcatctg tgccagacgg
      181 gactgctcaa cggcaagctg ctctgttgcc tgagcaagcc cttccgttac ggtcttcacc
      241 gaggtcagat ggtttatcat gaatccgtca ccggtcgtcc agcccgggtt cgccagaaca
      301 tactgatatc ctgcgatttt ttcctgcagg gatttcaccc gactggcctg ttcatcaatc
      361 agccggttct gctctgtcag cgccgcccgt gttcgtcctt cattatctga ggcttcaggc
      421 aaagacattg acggcgtttt atgcgcgatt tcatctatcg tcagtgcata ctggcgcgca
      481 gactccctgg cctgctcctg attctggtac agcgtgtacc atgctgcagc ccccagcatc
      541 accagtccgg gtacgccacc aaccagcccc agcgcaccgc tcatcagacg tgagcccacc
      601 gccgttgtac tgttcagcgc attctgggcg gcgcttctgg cagcaatatt tctgttcagg
      661 cgttcctgtg tggccgccag acgggcctct gcagcaatct gcatctccgt cccgcgggct
      721 gccgccacgg cctgctgagc acggtacacg gctgcccttg cccgcgccgt ggcaatctgc
      781 gttcccctga actgtgcttc cgccagtgca acttcattac gtgcagccgt cacaagtcct
      841 gccgtggcag acatcgctcc ggaggccata ttgccaaagt accgggccac cccgacggca
      901 accagcgcac ccacagctgt tgccacatta tcaatctgtc cggcaacacc gttcagcatg
      961 ccggagagcg tttttgtcac cccgctggcc tcattcgcac cgcccaccca agccataaag
     1021 gcgttttcca cctttgtgat cccgtcagag accgtttctg gcatggcggc atattcatca
     1081 cgcaatgtcc ccagctggct gattaacgcg ggcacgactt tatccgccgt cagttgacca
     1141 tcgtccgcca tcgccttcag atctttacgg gccacgccca tgcctgcagc cagtgcacga
     1201 atgatccggt caccactttc attgaccgaa ttaaattcct caccgcgcaa cacaccctgt
     1261 gccagcgcct ggctgaactg ggtgatcacc gaacccgcct cagccgtact ggcaccggag
     1321 attttcagcc ccgtggaaat ggcctccgtc accttcagta catcaccggc actgtaacca
     1381 tattcacgca tcgaggcagc cgaacgggca aacagggccg cattatccga aaaagcggta
     1441 cctgtccgct gactgatatc catcagcact ttctgtgatg acgaaaattc atcggatgac
     1501 tgtgatgcct gtttcagacg ggcattcacg gaactccact catcagccag tgaaatcagg
     1561 tgtccggtgg caaaggcacc ggcaaatgcg ccagccattc cgacagccga agcgcggatt
     1621 tccgtcaact ggctgttcag ttctgccagg gcacgtcgct gctcccgggc tgccgcagcg
     1681 gcctgacgcc cgccattctg cagggtccgg taatattcac tgcccatacg ggaagcccgc
     1741 tggatctccg actggaatga ctgtgaattt gccgaaattt tgataatcag ttcacgtaac
     1801 gtcgccattc acctttctcc gggcgtaaaa aaaccgcctc agcggttctc atcattcatg
     1861 actgtgctgc aaagctcagc gcgtcttcca gcgccgcaaa cggatccacc tccggcttat
     1921 cctcatcctc gccccagcag agcatggcgt ccttcagtgc aacattcatc ccctgtgccc
     1981 cgaaaaccgc tttcacgatc tgtgcattac ggatatcccc gcgctcatca cccagcgggg
     2041 acaccctgtc aaactccatc cacatcatcg ctttgctcgc actcaggctg tgccgcagtt
     2101 cggataaggt gcgccccaga cggagcgcaa gtcgcatcag aaagcgaatt tccgggcggg
     2161 ctactttttt ctggccgact ctgcatcagc gatcagttcc agtgcctgac gcagcaaccg
     2221 ggcatgtacc ggaccataga cggccagcac ctgctcacgg tcgtccggag tgaacacccg
     2281 ttgcaggtca gtatcacaca ggacatcgca gaacagcgtc acatccgctt ccaggttacg
     2341 gcgggttttc gccaccaccg acagggtatc gtcatcctct ccatcaccat tgagcacttc
     2401 ctgccacaga taccaggcct ctgccgaagg ctcccgcagc accacgctga catttcccca
     2461 ttccggcacc ttcaccgttt tatgacggaa ccctgacagt ctggccagcg ccagtgtttt
     2521 cagatctttt gccataagcc ttatccgccc gcaccattaa ccgttactgt acacgcatca
     2581 gaggtaatgc tctgcggctg ttctgcagaa tccgttacct cgcaggtata agccccctta
     2641 tcacctgact gcgtattggc tttactgaaa gtgtcagtag tctgtccctc taccggctga
     2701 ccatccttct tccaggcgtg tttataaggc ggcgttcccc cgttgacact gactgacatt
     2761 gtcagcagcg caccggtatt cacggtaagt gtcttctcca gatttttcac aaacgccagc
     2821 ggtaccacat aggacaccgg tttacccttc aggcgaagtg aaaacgttgc agccaccacg
     2881 ccgttggtac cggatgacca ggtgtgctga cgcacttccg ccaggaactt aaagccctta
     2941 ccggacggaa actgcacctt aaacgcatac accgtgtcat tgtcataggc atcacgcagg
     3001 gcgttctggg cctgattcag ataaaaatta cccgacatgg aaatctcgga cgacgccccc
     3061 agaccgttga tgttctcctg ctctgtggag cagagcgtgg tcacatcaat atcctgtttc
     3121 tgaccggcgg tgaactggac ttccttgatg gtgcagtcca ggcgcagata ttccgcctta
     3181 tccatagttt cagcagtcgc cggggcagat gaaatcatca cctgcgtcag ctgtgagcgt
     3241 tcatacaaag cagacattct gcctcctgat aataaaaaac ccgcacgcgg cggggtatgg
     3301 gttttgtaga aaaaaagaaa aagtcacacc gtgacctgaa actccaaggt tgcacggtaa
     3361 cagcggtttt ccggaatata gtcctgcatt tcactgacgg atcccggggc cagcagcatt
     3421 atggcttcac gggcgtcctg acgtatctga cgcgcctgcg tcacagtccc ggcataaacg
     3481 tctatctgca ccgacactga ggactccgcc tgcccgccca tcacgtccgc agacaccgat
     3541 gaaatcaggc tgaaaaccac ccacggaagc gccaccgacg gcctgccatc cagcaggggg
     3601 accacatacg ggtacacctg cccgccggca agatgcgcca gatgaggata caaatccgcc
     3661 tccgtcatcg tctcagtacc tcatcaatgg cccggttcat ccgagcaatc gccacctgcg
     3721 ctgcctgttc actgcgcaca tcaaatgccg gacgcacaaa cgggtgcggt ggcatattca
     3781 cggtccccat ttccacaaac cgccagtaga aagcattgcg cgggttatcc gccttcatgg
     3841 tgttatcgct gttaccggtg tccggattaa caccacggat atggacaccg gattccatcc
     3901 cgccatcgcg ggagcgccgg gaaaggacca ccacattgcg gcgcagtttc cccctgcgta
     3961 ccggtgcccg tgacaccact tcttctttca gcacattcgc ccccgcacgg gttgcctcac
     4021 gcagcacccg gttattttcc gcaccactca gaagctgcaa atcgcggctg atgtcctcca
     4081 gccccgaaaa atccagcagg gtttcgatca tttttcccct cccagccgac agagaatttc
     4141 cagacgccca ccggtcgcat ccggcacggg cagcccgaca acgttcagga tccggtcacg
     4201 ccatggacca ctcagcacat gaagtcgtga cgctgccgtg atttcccggc cggactgacc
     4261 gcgcacccag atgcggattt ccgcctgcgc catttccgca ccggactgca tccgctcccg
     4321 gctgctcctg ccacggatat ccgcatgaat tttcccgcat gacacccatt cttccgtcat
     4381 ttctccggca gcattacggg ttaacaccgg gttcagaaca cttatcatct gtgtcagacg
     4441 acctgcagat attgccattc cccctcctca taacaccgtc ggacaacgca aatcgtaaat
     4501 cagcacggaa acagaaaacg gcagctcccc ctgaatcagt tcttcccgct ccgcaagatc
     4561 cggattccgg tacagcatcc cggtcagtcg catggcagcc cccttcatcc gggttaatgc
     4621 ctcgcccggg atcagttcac cgtcctcacg aatcacttta tcccggctgc cctgaatgta
     4681 ggccagcagc acggcggtag cctgacgaac cttgtccatc agcatgtcat catccgcgtc
     4741 atggtcgaca cgcagatgtg ccttgatctc ttccagtgtc agtaatgccg tcattttccg
     4801 cctcctgcat cccgtccccg tttggcagcc agcgtccagc cagatgcatg cgcatccccc
     4861 ggtttatcgg cggtcatact gttgcagtgc cacagcgagc ccccccacgt caccgtatca
     4921 ccggggtggt aggtttcacc ggctctgaac acaccgcggt agagcatcac cagcagggaa
     4981 aatgtttttt ccgtacgctg gccactgctc tgccggacca ccacagagaa caaccgttca
     5041 cccgtcatgc tgacgtcaat atccgccacc ccgtcaacca ggcattccca tccccgcatc
     5101 ccgtgcgttt tttcatacgc ccgccagagt ccgccctggt gtgtggcata cgtgccccgg
     5161 ggaaaggatt tttgatcgtc aatggcgggg agtatttcca gtgccgtggc atcacgcccg
     5221 tcctgcggag ccggcagggc actcaccgca tccagaaccg ccttctgcag aacatccgga
     5281 tcgtagtcac gaccatcacg cggaacagg
//
LOCUS       DADLSA010000216         5285 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.217, whole genome shotgun sequence.
ACCESSION   DADLSA010000216 DADLSA010000000
VERSION     DADLSA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.217"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..410
                     /locus_tag="JN691_003910"
     CDS             <1..410
                     /locus_tag="JN691_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7245193.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            430..741
                     /locus_tag="JN691_003911"
     CDS             430..741
                     /locus_tag="JN691_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7245194.1"
                     /translation="MTLTLNELLLKLDRYRVIVVDDLGYVKRDSAETGVLFELIAHRY
                     ERGSLVITSNHPFSMWGSIFVDETMAVAAADRLIHHGYMFELKGESYRKKTAKAVTSV
                     T"
     gene            complement(910..5004)
                     /gene="sepA"
                     /locus_tag="JN691_003912"
     CDS             complement(910..5004)
                     /gene="sepA"
                     /locus_tag="JN691_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HAY7245195.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSWADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1297 a         1330 c         1229 g         1429 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc ggaagcgtat ctgcgccata ttctgagcgt actgccggaa tggccctcca
      361 accgtgttga cgaactcctg ccatggaacg tggttctcac caataaataa gcgtcaatac
      421 ggtgctccgt tgacgcttac actgaatgag ctgctactga aactggatcg ctaccgggtg
      481 atagtggtgg atgatcttgg ctatgtcaaa cgcgacagcg ccgaaacggg tgtgctgttc
      541 gagttaatag cgcatcgcta tgaacgtggg agcctggtga taaccagtaa ccatccgttc
      601 agcatgtggg gcagcatctt cgtggatgag actatggcgg tggcggcagc agaccgactg
      661 atccatcacg gatatatgtt cgaactgaaa ggggaaagct acaggaaaaa gacagcgaag
      721 gcagtaacaa gcgtgacttg atgtcgcact gaaggggcgg tcagtataat tggcgccagt
      781 cggcaaaact agttgacgtc taatacctgg acggtcactt ctgagaaaag gcatttacac
      841 agaatcttaa acaggctcac ggagttcttt cagaaaaagg cctgccgaat ggcaggccta
      901 tcccattcgt cagaaaacat aacggaagtt ggcgtttatc gcattatcca cattatattt
      961 cccgaacgct gatttctcca gctccagtcc caaacgcata ttatccttaa tttccgcatt
     1021 catccctacc gtcatcagca tcctgctatc tttttcacct tcgaaacgtt tctctccgga
     1081 agcatcctgc agaaccgttt ctccgttcgc cagcaggtcg aactgataac ccagcccggc
     1141 tcgagctgtg attttccagt cgtctccgga gaaggctctt cccacgtcaa caccagtacg
     1201 gccaatcagt gggttataat ccttgtcttt catgctcaga gccattcccc ggtcttccca
     1261 gctaaaagct tttcctgata cagaaccgta aaccagctct atctgtggct ccacccagga
     1321 ctctttcgtc aggtggtaac gataaccaac ttcagcaccg gcataccagg aatgagagct
     1381 gtagtctttt gttcccagtg aggcaaaatt cgccgtgtgc tgattatcat gatggagata
     1441 tttaccaatc aggtcaaaat aagctccgga attaaagagt gcagaggcat acagaccgcc
     1501 acccacggat ttgttttttc cactgaatgc gtggctgctt gcattgctgt ccgtataggt
     1561 cagcaatgcc cccgtaaata aatccacacc gtccagctca tgctttctgt cgacaccaat
     1621 ctgaacgtga gtgtagttat cgctgtagtc accatctgcc gaaccggtac cattcattat
     1681 gcgtgcccag acaccggcat ccccctgagt atcccgcagg tcacccatac gtttgttcag
     1741 gttattgacc tctgtaagga aggacttata acctgatgcc ataaagtctt ttgcggcttt
     1801 cgaggctccg gcatcagcgg tagtctgata tcctgtcagc acccattttg tggcatcatc
     1861 cgttttttcc gtgctgatta ctggcgtaac gttactgaaa ccaatctgct gcgtaccggc
     1921 agtgaacgtt ttctcatcac tccctttcgg tgcggtaatt aacgtcacgc tcatttcact
     1981 ggctggtttg tttaaaaagt caaccaggac gctattatta ctgcctgaca gtttgtctgt
     2041 gacattcaac tggtcagccc agctgttgtt ggtatgcatc aggaacgtgg tattatctgc
     2101 agaaagctct tttacttgta aagtggcaaa gtgtttcccg tcccccagtt caattcgcga
     2161 gcccttagcc ttcagtgcac cggcctctga ttttccgtca gagtaccaca gagcattgat
     2221 catatttacc gtactctgtg cccccgtaat actgcctctc agaacagcgt tatatccctt
     2281 cattaaggag ctgaataacg gagagtccgt cttccctgca tcagcatcac tgtctcccag
     2341 attaatggtt gccgcagtgg ttcctgcatg aatatccgaa ctcagatagc ccatattttt
     2401 aacagaaaag ctggcgttat cttcgaggtt aatcccttct gttgtagaaa ttacgggtga
     2461 gtaatacccc tgtttctgcg cagaaggcat ccccgtcagg gtcagagaag cattttcttt
     2521 tacatccaca gccccggtgc tgaaaatacc gctctgagcc gtaagttttg ctccttttcc
     2581 gagggtcagg ctgctgttga caaagctgcc aacacggtca aaaacagcat tctgagaggt
     2641 cacactcacg gtgctatcat aagcctcaat accaccggtg aatctctcca tgatttgcag
     2701 ggatgattta ttttccagcg taacatttcc gctgtaatca ctctggtcac caactgtggt
     2761 tgcagtggca ctcccttcaa cagaggagac cgcattaccg gttccgtcac tcctgtcagt
     2821 aaagacacgg ctacttccca gaatgactgt acttccattg gtggcgttaa tatctccctg
     2881 catcttgccg tttctggaca gatggaatgt tgccgcatcg agtttcagtt tttccagcgt
     2941 aaactgacgg gtttcccagt cgctctggct ggaagaagtc gtcgttccag catgaataac
     3001 tggatgaccc tggaatatca gcgtgccgga cttcttggtt atcgaacctt ctggcaggtt
     3061 aactgaacca tcaagagcca gtacatcctt acctgagagc tgcggaatat tcacatccat
     3121 attgccgttt agttggccgt gatacatata aggctggctg ctggcctcaa ttttttgttg
     3181 cttcacagta tcaatggctt tattacgatc ttttccgaca ttctgccaga cactgctgtt
     3241 gttcagatcg gagaagaacg gggaatatga actcgctttc aggatgaaat attgcttggt
     3301 agaactgtca taatataggt ctcccggcgc tcctctcccc ccaaaaatac tgacagtatt
     3361 aaccggtaca ttgatgtcac tggcttttaa tgtctgaagg tcgagtgtta ccgttgattt
     3421 tttctcactg ttattggtaa tgatggcccc gtagtcagcc gcctgaagac gggtaaaggt
     3481 cagattatta ccattcagtt ccagacgtcc tccacggtat ccccacgaaa tattatccgg
     3541 attaacttgc tgtgagtcag aaagcacaac tgtcggacga ccgctggcaa taccaacaga
     3601 gctgaaagcc tgtacctttc cgtcagcatc cgcctgctgg ttgagaataa cggtaccgtc
     3661 ccccaccttg agtccgcctg cattcacacc ggtcccgttt actgtcaggg taccttcgcc
     3721 agttttatgc aggttatcac cggcaactcc gttcacctgc cacagaacgt gggttccttt
     3781 atcggtgata atcccgcccc ccatccatgt ctggccgttc agggcggaca ccctgtaatc
     3841 gccggcaaac tgcagatacc cagctccctg attaacggaa ttctgcagaa cgacctcgcc
     3901 attattgccg gtgaacagga ggttttttcc tgcgttcaga tcatttcctt tctttccatg
     3961 catatcccaa gtcgtgtttc cctgagtcag tgtgccggtg ccattagccg catcatattt
     4021 ccactgcagt actccttcac cagaggtgta ggctatagtt ttgtcaaaat cattttgcgg
     4081 ttgctgacca aggaaatcct gagtggtgac gacccagtta tttccatagt tagttcctga
     4141 ggaaagcacg ccaatcagta cccatttttt ttgcagggaa tcataagcaa acagggggga
     4201 gccgctgtcc cccatattca gataattagc cagaggtcca ttcgcgggat taaaaatgtc
     4261 accggtctga gctgtaatca tctgtccgtt gttataggag ttcagcgcgc tgacggttcc
     4321 cccaacaaga aatccgccat tggtatacac ttgggtgcga ttaccatttt tgtctttgac
     4381 gtactgcatc ccccccccaa ggcgatagaa ttcagtaaag cgtccgccgg cctgataggc
     4441 accgctcaca gccccgatgt cagacacttc tgccggcgcc acctctgtca ccagtttact
     4501 caggcggcgg gttttaatat caagaccgct attattattt gtaccaaccg cagtataggt
     4561 gtttttggcg tatccgaagc tcatcgtgtc agaaccgctg acatgtttgg cggtaaccac
     4621 atactgaggg gagtaaagtg tatggtctcc cggaggcagg ctaccggtag taatagtggc
     4681 actactgaaa tcagccatcg gggctttatc aagtttaccg accagattgc cctgcttgtc
     4741 gtaaatggaa atattcgtgg tccccggcgt aaactgacct ttattctcgg caaagtcgcg
     4801 gaatatctga taagggatct ctgcactgac cgttgctgac agagctggcc atgccgaaga
     4861 aagtgataaa gctgcaacag acgtaagaat aacccgacgt gaaagtctgc gatggctttt
     4921 gcaggtaacc ctgcgggcca gttcggagac agcaatcagg cttttggtta tatggcaata
     4981 cttaagataa tatattttgt tcatatagaa acatcaacct aattttgaca aagattaatt
     5041 acataggatg tagatggagc agtccgagat tctactaata tcaattatta agcatctgtt
     5101 ttttagttgg tgtaatataa tttttattaa ggtaagcgga aaaacaacca aaaaaaacgc
     5161 aagatgttat ttttttatca ttttgagcca gtttggctgg gaggatgctc ttctcttaag
     5221 cttcttgctc aactttaaaa atagttatgc cagttcggta tcgataaggg gcagtttgga
     5281 tatcg
//
LOCUS       DADLSA010000217         5275 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.218, whole genome shotgun sequence.
ACCESSION   DADLSA010000217 DADLSA010000000
VERSION     DADLSA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.218"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(68..487)
                     /locus_tag="JN691_003913"
     CDS             complement(68..487)
                     /locus_tag="JN691_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245196.1"
                     /translation="MHQLRHPLLGCVFCNECDPHPFAPDPLQIFHHYYEWVRPCFPIR
                     AGNRFSRSLRWSGPGSCCLHTGCRAVSKQVSSALIRGRLYGPILTSSKISMLHRTVYF
                     RSASRTPRDSRSLPFPESLTTSFLRMKQHRVVWSVLL"
     gene            537..1715
                     /gene="ltrA"
                     /locus_tag="JN691_003914"
     CDS             537..1715
                     /gene="ltrA"
                     /locus_tag="JN691_003914"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY7245197.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1798..1977
                     /locus_tag="JN691_003915"
                     /pseudo
     CDS             1798..1977
                     /locus_tag="JN691_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2007..2489)
                     /locus_tag="JN691_003916"
                     /pseudo
     CDS             complement(2007..2489)
                     /locus_tag="JN691_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2745..3725)
                     /locus_tag="JN691_003917"
     CDS             complement(2745..3725)
                     /locus_tag="JN691_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY7245198.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3725..4924)
                     /locus_tag="JN691_003918"
     CDS             complement(3725..4924)
                     /locus_tag="JN691_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY7245199.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
BASE COUNT         1481 a         1186 c         1235 g         1373 t
ORIGIN      
        1 gttcggaaat tgcagcataa gttatccatg ctctgaggtg ggtggaggtg ggccctccgg
       61 tcagggttta caggagcact gaccaaacga cccggtgctg cttcattcta aggaatgagg
      121 tggtgagcga ctcgggaaaa ggcagagatc tactgtcacg tggggttcgg gaagctgaac
      181 gaaagtaaac tgtccgatga agcatcgaaa tcttagatga tgtcaaaatg gggccgtaca
      241 gccgtcccct gataagcgca gaagacacct gcttactgac tgcgcggcat ccggtgtgaa
      301 ggcagcagga tcccgggcca gaccatctca gggaacgtga gaacctgttt ccggcacgta
      361 tcgggaaaca ggggcggacc cattcgtagt agtgatgaaa gatctgtaat gggtctggag
      421 cgaagggatg gggatcacat tcattgcaga agacacaacc caaaagggga tgacgtaact
      481 gatgcaacga aaatcatttg aaataccaaa agcactggtc tgggcatcat atctggatgt
      541 gcgtcgcaac aagggagccc cccggatgcg acgggcagac tttgaaaatg ttcgaccagc
      601 aacgtgatgg caatctatac aagatctgga accggctatg ttcgggaaca tggtttccgc
      661 caccagtgct ggaaaaacgg atcccgaagt cgaatggcaa ggaacgcatt ctggggatcc
      721 caacagtatc cgatcgaatt gctcagggag cgataaaact tttcatggaa gaaaagcttg
      781 acccgatttt ccatgctgat tcatatggtt accgaccagg caaatcagct catgacgctc
      841 tgaaacaatg tgccatccgg tgctggcgtt atagctggat actggaagtg gacatcagtg
      901 cgtttttcga ccatgtgaga catgatctgg tgctcaaagc tctggaacac cacgggatgc
      961 ctaaatgggt catcctgtac tgtcgacgct ggatggaagc gccaatgcag agttgtgaaa
     1021 atggagaatt aataacccga acacgaggca ctccgcaggg cggggtcatt agtccgctac
     1081 ttgctaactt gttccttcac tatgcatttg atttgtggat ggaaagagaa tatcgggggg
     1141 taccgtttga gaggtacgct gacgatattg tagtacattg ttcacgaatg agtgatgcaa
     1201 caaggctaaa gaacaggtta tcggagcgct tctcggaagt cgggctggtc ctgaatgcag
     1261 ggaaaacgaa tatcgcctac attgacacgt ttaaaaggag aaacgtcgca acgagtttca
     1321 cctttctcgg atatgacttc aaagtgcgta cgctgaagaa tttcaaaggc gaacggtacc
     1381 gaaaatgcat gccaggtgcg tcaaatgcag caatgcgcaa aataacagaa acaatcaaga
     1441 agtggcgtat acatcgctca acagctgaga gtttgctgga ttttgcgagg cgctacaatg
     1501 cgatagtgag aggctggatc gagtactacg gaaagttctg gtccagaaat ttcaactatc
     1561 gactgtggag tgcaatgcag tcacgtctgc tcaagtggat gcagtctaaa tacagacttt
     1621 cgaaccggaa ggctcagcga aagctgacgc tggtaaggaa ggagtatccg aagctatttg
     1681 ttcactggta tttactacgt gcatcaaatg agtgatcaag agccgtatga cgcgagagtg
     1741 tcacgtacgg ttccgtgagc agccggggag tgaaattctc tccggctgac tcgactcgcg
     1801 ggttccgaca gcgatgacga acatgcggcg gtgttgtact cgctgatcgg cacatgccgt
     1861 ctgaacaatg tggaaccaga aaaatggctg cgttacgtca ttgagcatat ccaggactgg
     1921 ccggcaaacc gggtacgtga tctgttgccc tggaaagttg atctgagctc tcagtaaata
     1981 tcaatacggt tctgacgagc cgcttacttt tggcgtaatt ctgctgcgcc tttactgaac
     2041 acaatgccgt attcagcaag aagtccacga atgtgattta tcagttgtgt cctttccatt
     2101 gaaaccaacc gacgcatatt caaaaaagtc tggacgtcct gttgctccag agtttttatt
     2161 ggtacgggtc gtatagttcc atgttggcag gcctcagcta ttgcctgtgc gtcgttataa
     2221 tcatttttct ggcaccgctg ataagcttta acatgttgtg ccggaagcaa tattacatca
     2281 tggtcgagtt tcctgatttc ccgggcccag taatgagatg ccccgccagc ctccatcgca
     2341 acagtcattt taggctgttg caccagccag ttaagcagtt gattgcgaga aagtttggtc
     2401 tttcctgcgg gtttgccatg agcattgata ctgaaaagat aaaagttcgt tttagccaga
     2461 tcaatgccgg tgattttctg ttcagacatg gtatcccgct tacttttgtg gttaaagttt
     2521 atccatcatg gcgcaattga aggctgccgt gaaagagggg gaagactatc tcatcggtta
     2581 ctgttaaacg gctaagagaa actatctttt tcaagcaaag aagatacggt gaaacaggcg
     2641 atttcaccgt gaaatactgt tcaaaaaaaa cgctaataaa agcaaaattt cttttaaaac
     2701 agccaattaa aaactggtga gttgctggcg aaaaacttga attttcacag cgaattcaga
     2761 ttatctttta gtacaagagc aatcatacga tctaacttat cctgaacttc caacggtaat
     2821 ctgccgaatt catatgagaa cgtacgtccc tttactcgtt ttcgagcaaa tttatcttta
     2881 ctatcaaatt tccataacaa agttacaacc gctttatcct ttactcctga atcaagcaat
     2941 gaagacattt ccttggttat aagccgcata atactgtttt taacctcgtc ttcagaaaga
     3001 ttgtcatcag cagtaataag ctcaatactc ggcgagatat tcttcacaac ttcatcaata
     3061 gatatatcac ccagcctcag tcgttcactt aattcagcca gttgcctgta atcggcgtat
     3121 gtcagctcag actgaacagg aaaaagagac acaaaatctt taggtacgga cgctgcttgg
     3181 agggcacgag tcaccttagc tgccgacaat ccctctcttt ccgcaatttg cttctggttc
     3241 ataccggcct ctttgagacg tactaaacgc aacccaattt cacgaatatt atgttcaagt
     3301 gacgtctgca aatctttcgc cagatgctga gcttcagaaa cagtaagctc atcatccgtt
     3361 acaagtacac gtaatccaac cttgcatagc aaagctgctg cccgacgacg ggaaccatcc
     3421 aaaatctcaa taagatcgcc agtccgaatc ccaatacatg gatagaattg ttgtaaacgt
     3481 atagttctgg tgatgtcttt tagtgagtcc ggcgtcaggg ctgtctgttc gcggccattc
     3541 acctcttcaa ccacaaaagt ttgagtgtcc actttgtcat gatcaacctt gatctcagta
     3601 aattttgcct ttcggccagt attgagggta aatacatgaa ctggagccga tatttcttca
     3661 gtattattca ggatatttgt attcagcgta cggccaatag tcggtcggtg ctttctgttt
     3721 tccatcagta gttggccctt ataaattcaa tacgatcaaa aactgcttta gcgaaatctt
     3781 ctgctgccat acgtgcattt ttcaaagctt caccactgcc aacataagta acaggattcg
     3841 cagatatcac ggtatcaaac gactcgccgc ttctctcaaa accatcaaga cgtggcattg
     3901 aaacatcaag catgtcacca ccgaaaatct cttttgtcag gctatggcag tatttatgat
     3961 ctgacttatt cgccagtttc gacatgaagc caatatttgc ctgcaaacgg catgaacatc
     4021 catcctgttc aataatctgt acaagttcag gtaaacgagc caaatattta agcgtagaat
     4081 ggaaatcaac ttgtgctggc ggcaccggtg taaacatgat atctgcggca gcaatggcat
     4141 ttttcaaaaa tgcgtccaga tgcgggcctg tatcaataag gatgaagtcg aaatcatgct
     4201 tcagcttatc aataatattc tcacgcaata tcgcgtgttt gttttgtcct aatagatgct
     4261 cctcgcacag agtatcccag tttgatgcaa taaaggcgtc gtcaatagaa gccggcataa
     4321 cataaacgcc agggataact gacggaacga taaaatcttc cagtaattcc tcacgactca
     4381 cgttctgcaa catagcctgc ggtgcggttg tatcaaccag ccccaccgca tgcaagtagt
     4441 tcagaaacat agtggctgat gactggggat ctaaatcaag gagcagaata cgtaaatctt
     4501 ctgacagcag gtgaggatga gcacgcaggg cgtgagctac ggaaacacta ctcacggttt
     4561 tagacacacc gccttttaaa ctaccgatga atatggaata tgcctcacta taacgatcgc
     4621 gatatttagg aatgccacga tgtgcataga tatcgataat gttctggatg gttaatgcat
     4681 attttgtcgc ggttcctgct gggcgttttt caaactgata gccctgagcc tccatctcac
     4741 cgactgcacc atccacgctt gcgcgcgtta attttggaag cttagctaca gcagactttg
     4801 aatagacttg ataaaaaaca tcagcctgaa actcctgctt ttgctcctga atctgttttg
     4861 ttagggcgag caacatttta tctgcacgct gcgcaacctt acttaactgc tcaaaactag
     4921 tcattccccc tccgtgctat gtaggatttt aacttattac aaaaaaaact gcattagcag
     4981 ggtaatgcat gcaagtacat attgtacatg cttatattaa aaatgcgcgc attgctgatt
     5041 tattaatcat cggaatgaat attactgtaa gatagtatgc ctttcgggga gaaagacgct
     5101 gatttccccc gaataatcca gttcaccacc acactccgga caacagcttt cttcaggctc
     5161 cagatggtgt atttcgcggg aaacatgttc cggtaacgga cggcgatgac gtgactgtcg
     5221 caacaggcgg gggtagtgct tccaatcagt agaacatgtg cttttcaatg aagga
//
LOCUS       DADLSA010000218         5168 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.219, whole genome shotgun sequence.
ACCESSION   DADLSA010000218 DADLSA010000000
VERSION     DADLSA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.219"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            11..596
                     /locus_tag="JN691_003919"
                     /pseudo
     CDS             11..596
                     /locus_tag="JN691_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            609..1367
                     /gene="chbG"
                     /locus_tag="JN691_003920"
     CDS             609..1367
                     /gene="chbG"
                     /locus_tag="JN691_003920"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAY7245200.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(1414..3665)
                     /gene="katE"
                     /locus_tag="JN691_003921"
                     /pseudo
     CDS             complement(1414..3665)
                     /gene="katE"
                     /locus_tag="JN691_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
     gene            3848..4111
                     /gene="cedA"
                     /locus_tag="JN691_003922"
     CDS             3848..4111
                     /gene="cedA"
                     /locus_tag="JN691_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HAY7245201.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            4388..5168
                     /locus_tag="JN691_003923"
                     /pseudo
     CDS             4388..5168
                     /locus_tag="JN691_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1321 a         1131 c         1309 g         1407 t
ORIGIN      
        1 tctgaatctg ttgccgtgtt cttatttgct ttattacttc aagcaaaaag agatgctggc
       61 tattgaaatg ggcgaatact acaaaggcgg cgcacgagcg caggtagtac agaaagtcga
      121 gaaacaactt tttgagctgt ataaaaaccc ggagttgaaa gttaagccga aagaactgga
      181 acagcgcggt ggggcttatt actctgatgc agcgtgcgaa gtgatcaacg ctatctacaa
      241 cgacaagcaa gctgaacatt acgttaatat cccgcatcat gggcatattg ataatattcc
      301 ggcagactgg gcggtagaaa tgacctgtaa gctggggcgc gacgccacat ccgcgcatta
      361 cgcatttcga tgataaagtg atggggctga ttcacaccat taaaggcttc gagattgctg
      421 ccagcaacgc cgcacttagc ggagaattta acgatgtgtt actggcgcta aaccttagtc
      481 cgttggtgca ttccgatcgc gatgctgagc tgctggcacg cgagatgatt ctggcgcacg
      541 agaaatggct gccaaatttt gccgactgca tcgcagagct taaaaaagca cattaaccga
      601 ggctgattat ggaacgctta ctgattgtta atgccgatga ttttggctta agcaaaggtc
      661 agaactacgg cattatcgag gcctgtcgca atgggattgt cacatcgacg acggcgctgg
      721 tgaatgggca ggctattgac catgcggtgc agttgagtcg tgatgaaccg agtctggcca
      781 tagggatgca ctttgtcctt actatgggca agccactgac agctatgccg gggttaaccc
      841 gcgatggtgt gctgggaaaa tggatctggc agttggcaga agaagatgct ttaccgctgg
      901 aagaaattac tcaggagctt gccagtcagt atttgcgttt cattgagcta tttggacgca
      961 aacctacgca tcttgatagc catcatcatg tgcatatgtt cccgcagatt ttcccgattg
     1021 ttgccaggtt tgcggctgaa gaggggattg cgttgcgcat cgaccgtcag ccgctatcta
     1081 acgctggtga tttaccggct aatctgcgca gttcgcaggg attcagtagc gcgttttatg
     1141 gtgaagagat tagcgaggca ctgttcctgc aagtgctgga tgacgcaagc catcggggtg
     1201 atccttcgct ggaggtgatg tgtcatccgg cgtttatcga taatactatc cgtcagagtg
     1261 cttactgttt cccacggtta acggagctgg aagtgctgac atcagcgtcg ttgaaatatg
     1321 caattgctga gcgtggttat cggttgggta gttatcttga tgtgtaagta aggtggtaag
     1381 gtcactcccc aggggacgtg accttatcca tatttacgcc ggaattttgt caatcttagg
     1441 aatgcgtgac cacacgcggt gttctgccat cagcgttaac agttcatcca taaaactacc
     1501 gtcggcgctg tcagcttcca caatcccttc ttcaccctgg tcagctacct tgattgttgc
     1561 tttaaacttg cgcgcgtctc ccgccagcgc aatcggttta aggtgtttgt aggcttccat
     1621 cagatagtag ttggcatcgc cgttgtcagc gatatccgcg atattgccgc aagggacaat
     1681 gaccgcatcg accgtcagcg aaggtgcacc ggcaaaggta gcggctatag gcaacaccgt
     1741 accgtcatcc gcagtcactt cacccactcg ggagtagagc agtttggcat gaacgccttt
     1801 ggccttcagg gccttgagaa tggccagaag gtctgccgat ctcacttcat cattaagtaa
     1861 aatcgctacc acgcgacctt tcacatcacc gtcaggaatg gcgtacaaac ttaaggatgg
     1921 atcctttttc agaccgttga cgtccggagg tggggtgata ttcagctggt cgtcagtcag
     1981 ttcgataccg agatttttcg ccaccgcctg ggccagagtg agatcaatat gcgccagctg
     2041 gtcaacaacg cgctcacgaa tatacggacg aacgactttg cttaactcaa aactgaaacc
     2101 atcgacaata tggcgctgct cgaatggcgt ctgacttagc cagaacagac gcggatggga
     2161 ataatattcg ccaaacgatg ggctgcgctc gcgaacttta ttgccttcca cgcgctcctg
     2221 gtatgattca aaaccgccgc gtttcggccc cggtggtgtt tcgcgcggcc agttatcgtt
     2281 gatcgagttc ggttcataat tcgccgggtt agtgtcgatc cccatacgat gcatgccgtc
     2341 acgctggaaa ttatggtaag ggcaggtcgg gcggttaatc ggaatctcat ggaaattcgg
     2401 cccaccaaga cgactgattt gtgtatcggt ataggagaac aaacgtccct gcaacagggg
     2461 atcgttggtg aagtccagac caggaacaat atgacctgga tggaatgctg cttgttcgtt
     2521 ttcggcaaag aagttatccg gattgcgatt gagcaccatt ttgccgacac gctgaacggg
     2581 caccagttct tccgggatca gtttggttgg atcgagaaga tcgaagtcga acttgaattc
     2641 atcttcttca ggaatcaact ggaagcccag ttcgtattcc ggaaaatcgc ctgcttcaat
     2701 ggcttcccac aactcgcggc ggtggaagtc cgggtcacgt ccggtgagtc atcccaaacg
     2761 agtgaggctt tacctgccag tggtttccag tggaaacgta caaacgttgc cttcccttcg
     2821 gcattaatca ggcggaaggt gtgaataccg aagccttcca tggtgcggta actgcggggg
     2881 atgccgcgat ccgacatcgc ccacatcacg ttgtgcagag tttcaggttg cagagaaaca
     2941 taatcccaga aagtatcgtg ggcactttgc ccttgtggaa ttgcccagtg cggttctggt
     3001 tttaccgcat gaacaaaatc ggggaattta tgcgcatcct ggataaagaa gattggcgtg
     3061 ttattgccaa cgaggtcaaa aataccctct tcagtataga acttggtggc aaaaccacgg
     3121 atatcacgca cggtatcggc agagccagcg ccaccctgaa cggtagagaa cctaacaaat
     3181 actggggtga ttttgttcgg atctgagagg aaatccgctt tggtgatatc gcttaagctt
     3241 ttatatggct ggaaataacc gtgagctgct gatccgcgtg catgaacaat acgttccggg
     3301 atgcgctcat ggtcaaagtg ggtgattttc tcgcgcagaa taaaatcttc cagcagcgtt
     3361 ggaccacggt taccggcacg cagtgagttt tgatcatcgg cgatgcgcac gccctgatta
     3421 gtggtcagcg cataattttc actgcctttg cgtacgtctt ccagagaatt aagtttttcg
     3481 ttacgtgtat caggggcttt caggctccca ggggcggtag gttgtgcacc tggcggtgtt
     3541 ggttcagccg ctggacgatg agatccgtcc tcaggtgcca gtgagtccat ccccggtttc
     3601 gcttcgctgg aatcgtgtag tggtgactgg tgctgatgtg ggttcttttc gttatgttgc
     3661 gacattgaac tcgtctcctt aatttattac tgaaagggcc gctgttttgg tcaaaaaact
     3721 ctctaactat agaccaccag ccagatcccg cttcggagac tattctggaa acggtgttat
     3781 gaaatacacg caaagcggga cgtacagggg ctaaatcggc taaactacag tttttctaat
     3841 ttttaatatg cgtttagtga agccagtaat gaagaaaccg ctccgccagc aaaaccgcca
     3901 gattattagc tatgtcccac gcacggaacc cgcgccgcca gaacatgcga taaagatgga
     3961 ttcgtttcgt gatgtctgga tgctgcgtgg caaatatgtt gcgtttgtac tgatgggaga
     4021 gtcatttctg cgctcaccgg cgtttactgt gcctgaatct gcccaacgtt gggcaaatca
     4081 gatccgccag gaaggggaag tgactgagta aggattacat acctcccggt tttgccggga
     4141 gttacatgac ctgaaattct gcatttcgtt gctacattca ttcaaaccac ctgtaacaaa
     4201 aagctgcctt tgacgagcta ttgctataaa taagtgatct tttttcttta attgtttatt
     4261 tattttaaaa tggttaagtt ttgctaatca tgatgagcta tttttgatag ttcatcctgt
     4321 ggcgtcgctc ttaaacttat attgtaaatg acttagattt aaataagata ataattttgt
     4381 taacaatatg aggcgggaga tgttttctac aaacttaata cagagtaatt atggtgattt
     4441 aaatattaaa agcctggctt tcgactcttt taaagaaagg cttgagtcta ctatggcggc
     4501 actcactttc tttatatcaa cgggtcaatg tgactgcgat gaaatagcag agtcgaactt
     4561 caatcgtatg attgcctata tgtctaacat taactatgat gcctcaaagc taggagcgcc
     4621 agcacttagt tttgatacgt atctgcagga caatgttaaa tatagagtga taattaataa
     4681 tctttatggc agtgagtttc ggataagaga tattaatgag aattttgttg ggatggattt
     4741 tacctgtgaa ttccgacccg aaaaaatgac gaatttgatc aatataaaaa ataggttggt
     4801 tattcattat ttaaggacta tatactatga aaaaaattat atacatcctg ccgggagcat
     4861 ttttgctgcg attcaaaaaa aatgagagtc tcctaaagtt tccttcgata atatcaatgc
     4921 ttaatatcaa tcttcttttt aatccactaa atcttccggg aatgggaagt ggtgtgcttg
     4981 aagatattat gagtattcca gatagttcac ttagaaaacg tctcgggtat gaggttttaa
     5041 gcttctcact acaggcacat tcattgagcc aggaatgtat tgataagttg gacatttttt
     5101 tcgctgacga tctgtttaaa tatgagtcag tatgcatcgc cgcgatggaa catttaaaaa
     5161 gtaaagca
//
LOCUS       DADLSA010000219         5153 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.220, whole genome shotgun sequence.
ACCESSION   DADLSA010000219 DADLSA010000000
VERSION     DADLSA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.220"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            135..554
                     /locus_tag="JN691_003924"
     CDS             135..554
                     /locus_tag="JN691_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001711305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF342 domain-containing protein"
                     /protein_id="HAY7245202.1"
                     /translation="MAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRREFETS
                     ELIRAYGELKRNETPERHTQGHSEKPHDQQIERILRELNELKQCLTLMLEDKQAQDTE
                     RRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
     gene            635..1054
                     /gene="exc2"
                     /locus_tag="JN691_003925"
     CDS             635..1054
                     /gene="exc2"
                     /locus_tag="JN691_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HAY7245203.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGF
                     DPNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGT
                     TQFAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
     gene            complement(1279..1602)
                     /locus_tag="JN691_003926"
     CDS             complement(1279..1602)
                     /locus_tag="JN691_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HAY7245204.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1886..2080
                     /locus_tag="JN691_003927"
     CDS             1886..2080
                     /locus_tag="JN691_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY7245205.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHEL
                     AEELQNSIQTRFEVENSTER"
     gene            2268..2639
                     /locus_tag="JN691_003928"
     CDS             2268..2639
                     /locus_tag="JN691_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245206.1"
                     /translation="MSKKQSTGRSQRRRRFSIGSAPLTSITKIDAQARGGETRQDYKD
                     TRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFLIAHAVGISARCRSFAPSWAVCT
                     NPPFSPTAAPYPVTIVLSPTR"
     gene            2567..2725
                     /locus_tag="JN691_003929"
     CDS             2567..2725
                     /locus_tag="JN691_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7245207.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLAPLAAAVGNLVSRAMM
                     AQMVQQSS"
     gene            complement(2922..3059)
                     /locus_tag="JN691_003930"
     CDS             complement(2922..3059)
                     /locus_tag="JN691_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 lysis protein"
                     /protein_id="HAY7245208.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISV
                     Q"
     gene            3107..3448
                     /locus_tag="JN691_003931"
     CDS             3107..3448
                     /locus_tag="JN691_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HAY7245209.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKILYAIV
                     ISTILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFG
                     LLGLFISIKNN"
     gene            complement(3445..5010)
                     /locus_tag="JN691_003932"
     CDS             complement(3445..5010)
                     /locus_tag="JN691_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-10"
                     /protein_id="HAY7245210.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSK
                     SESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEAL
                     RHNSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIE
                     KEQAETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRL
                     SSSIHARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLR
                     ARAGDEMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKT
                     AQTNLLNSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAF
                     EKYKDVLNKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDI
                     LKIKDTGDWKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFID
                     KNKLNTINEVLGI"
BASE COUNT         1457 a         1291 c         1062 g         1343 t
ORIGIN      
        1 aaaaaaacag ccacccacac cacgtgctat ttccacccga tgccacaaaa accagcacaa
       61 acattaccgt tctcagacct cattatgttt tactgaaacc atgagatgag acacctatgg
      121 gacactgtaa ctttatggca tggcacacac tccgggacgc actgaaaatg actggaagat
      181 cgcgttcaca gttttaccgt gatatgcgct caggtcttgt cagttaccgt accagccagg
      241 acggacgacg ggagtttgaa accagtgaac tgatccgggc atacggcgaa ttaaagcgga
      301 atgagacacc agaaaggcac actcaaggac actcagaaaa acctcatgat cagcagatag
      361 aacgcattct ccgggaactg aatgagctga aacaatgcct gacgctgatg cttgaggata
      421 aacaagccca ggatacggag cgccggcgcc aggaagcaga acggaaacag ctacagaatg
      481 agatagccca gctcaggcag gctctggagc aggaaaagaa acggggattc tggtccaggc
      541 tgttcggtca ctgaacgcgc tcagaaactg atgataagat aatcttcgga taataactca
      601 ccgagaataa atactttaag gtagggagac actcatgaga cgtaccggaa acaaactttg
      661 tcttatcgcc atgataacag caacagtagc tctcacagcc tgtaccccaa agggcagcgt
      721 ggaacaacat acccggcatt acgtatatgc ttctgatgac ggttttgatc ccaacttttc
      781 cacccaaaaa gccgacacaa cacgaatgat ggtgcctttt tttcggcagt tctgggatat
      841 gggagctaaa gataaagcga caggaaaatc acggagtgat gtgcaacaac gcattcaaca
      901 gtttcacagc caggaatttt taaactcact ccggggcaca actcaatttg cgggtactga
      961 ttaccgcagc aaagacctta ccccgaaaaa atccaggctg ctggctgaca cgatttctgc
     1021 ggtttatctc gatggctacg agggcagaca gtaagtggat ttaccataat cccttaattg
     1081 tacgcaccac tgaaacgcgc tgaacgcgat tctgacgcag acaccgcaaa aagagatgtt
     1141 ttaccccgga aaaacaggaa ggatgcgtca ggatcgtttt cagctctctg catagctatg
     1201 catgaaagcg aatggtgatc actttgggag cttacggtgt tcataccgtc agttttcgac
     1261 agttttctct ccgggaagct aatctgccat aagcctggat aacagggcac ggtgatactc
     1321 cgtaatagcg atcagcacct ccgcatactc cctgtccccg acacgcttgc cgtcgataaa
     1381 gagttttttc tgtaacgccc ccagtctcat aacttctttc agtacccgat catcagtcag
     1441 tgagttaact ttcttaccga gagctgccgc ccgtaaaaaa ccggaaacgg tcttgccgca
     1501 ttcagcagcc ctttttctga tttcctggtc ttcttctgcc gtcagacgaa cagggcgact
     1561 gatagcccgc ctgcgcgtat tacttccact tcgttttgtc ataaaacatg gctccgtatc
     1621 tgacatgggt gtcggggcaa agccctgacc agggtaattg taatagcgtg catgtatgcg
     1681 cggtataaca attacacatc ctgtcctgtc agcaagttcg aattaccgac aaaggcacgg
     1741 cagttttttg gcagtgacag tattgtcagg cagaaatacc cgtcaaagcg tcaaacgaca
     1801 gaataccggc ggtaaaacag tcgaaaagtg gtacgcgata taatcacaca acgcagatga
     1861 cagacagaac ggagaaaacg aaaagatgaa caagcagcag caagccgtac tcaacatggc
     1921 aggattcata aaaagccaaa gcctgacgct gctcgaaaag ctggacgcac tcgatgctga
     1981 cgaacaggct accatgtgtg agaagctgca cgaactcgca gaagaactcc agaacagcat
     2041 ccagacacgc tttgaagtag aaaatagcac agagagataa cgctcaggac aggatgccga
     2101 acggcacaac agagcgtaag gagaaaatgt cacataaggc gctcttccgc ttcctcgctc
     2161 actgactcgc tacgctcgga cgttcgactg cggcgagcgg tgtcagctca ctcaaaagcg
     2221 gtaatacggt tatccacaga atcaggggat aaagccggaa agaacatgtg agcaaaaagc
     2281 aaagcaccgg aagaagccag cgccgcaggc gtttttccat aggctccgcc cccctgacga
     2341 gcatcacaaa aatcgacgct caagccagag gtggcgaaac ccgacaggac tataaagata
     2401 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2461 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2521 ttggtatctc ggctcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2581 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2641 acacgactta gcgccactgg cagcagccgt tggtaacttg gttagcagag cgatgatggc
     2701 gcaaatggtg caacagagtt cttgaagtag tggcccgact acggctacac tggaaggaca
     2761 gtatttggta tctgcgctct gctgaagcca gttacctcgg ttaagcagtt ccccaactga
     2821 cttaaccttc gatcaaaccg cctccccagg cggttttttc gtttacaggc agcagattac
     2881 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac agagatcccc
     2941 gtcaatttag aagaagatga tggagccaca gtccctccct gaacatcacg tatatagtta
     3001 gcctgacatc caacaaggag gtttatagcg aatattccca caaaaaatct tttcctcata
     3061 actcgatcct tataaaatga aaagaatata taacgaggtt taatttatga gtttaagata
     3121 ctacataaaa aatattttgt ttggcctata ctgcgcactt atatatatat accttataac
     3181 aaaaaacaac gaagggtatt atttcctagc gtcagataag attctatacg caatagtgat
     3241 aagcactatt ctatgcccat attcaaaata tgctattgaa cacatatttt ttaagttcat
     3301 aaagaaagat tttttcagaa aaagaaaaaa cctaaataat gcccccgtgg caaaattaaa
     3361 cctatttatg ctatacaatc tactttgttt ggtcctagca atcccatttg gattgctagg
     3421 actgtttata tcaataaaga ataattaaat acccaacacc tcatttatag tattaagttt
     3481 attcttatca ataaaggcac atagaatgcc tgtaacaata gcaatccccc agatacctaa
     3541 tgtagttcca gcaagcacac taaaaagtaa aacaacaaca taactaactc cagcatctac
     3601 agctttcttc tctaatgtaa gaaatagcgg cttccagtca cctgtatcct taatttttag
     3661 gatatcagat accacatcat atccaaaaga aacatgtccc gtaatcttca agtacttggc
     3721 aaactgatct aaatgcttag cccagtcttc atacttaacc gcctccagtg cattgaaaat
     3781 tgcatcacgg tctgctttgc tgaatttctt atttaataca tccttgtatt tctcaaaagc
     3841 agctagagct tcattcacat tgcttatttt cttaccttta gatttatcag caagttcctg
     3901 tgccattttt gaatattttt caccatattt ttcagatagc gtttgataaa agctaactgt
     3961 tgcatccaca gcatccttaa tctgcgagtt caggagatta gtctgtgctg ttttcaaatt
     4021 atcttcagca tcatgaatac gtgaaactgc tgtacttcgc ttattattag cctgagaaat
     4081 agcctcttgg attccattta tctcagagct aatctggtta agacgcgttt ctgatgctgt
     4141 tacctgcttt tgtttttcct ccatctcatc accagctctc gcccgtagtc tggtggcctc
     4201 aaaaaaagga cgactctgaa gcggatcatt cgccctagga gataaaagtt taaccctttc
     4261 atccagttct ttatatttag cagatgcctg atccaactca tttcgttttc cggacagcgt
     4321 attcgtttct gcatcacggg catgaatact ggagcttaaa cgggtattga gcgtattaat
     4381 ctcttcatcc actttcgcca gctcagattg tgcagcagca agattttttt gtgccacctc
     4441 cacagcccgg gcctcttcac tcaatgctgc caggcgtttc tcttcatcct cagccagttt
     4501 caattggcgt tctgtttcag cctgctcctt ctctatctct ttacgtcgtt gttctgcttc
     4561 ctgaaaagcc ttttccgcag cttccgcttc tttacgggct ttttcttctg cttttgcaag
     4621 gcgcaaccgc tctgcttctg cctgcatcgc tgcattattg gcatgagcca ggtcaataac
     4681 ctccggatga gtggaattat ggcgaagcgc ctcattcaca atatccttca gatgttgagt
     4741 cagcgcatcc cggtttgctt ttgctttagc ctgtgcttct gcggcagctt ttgctcgggc
     4801 agcctgttct gcctgcgttt tcttcaattg agcagtagac catttagctg tggcatgaat
     4861 ggctgcagaa ctttcacttt tgctacctcc agttccacca ccaccgccag agccactccc
     4921 gtctggatta ccattcaaaa gagtaatgat tacctgcccc ttatcatcat aaggaacacc
     4981 atctttatag tacgctacag ctgtttccat tataaaatcc tctttgacat taaaaacaaa
     5041 tagttaaaag aaatatactg tatatataat cactggtttt atatacagtt taaaattacg
     5101 ccgctgcatt ttccctgtca accctgtgga ttttcatttt tgtgaaaacg atc
//
LOCUS       DADLSA010000220         5088 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.221, whole genome shotgun sequence.
ACCESSION   DADLSA010000220 DADLSA010000000
VERSION     DADLSA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.221"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(129..204)
                     /locus_tag="JN691_003933"
     tRNA            complement(129..204)
                     /locus_tag="JN691_003933"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(169..171),aa:Thr,seq:tgt)
     gene            complement(210..284)
                     /locus_tag="JN691_003934"
     tRNA            complement(210..284)
                     /locus_tag="JN691_003934"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(249..251),aa:Asn,seq:gtt)
     gene            complement(545..1168)
                     /locus_tag="JN691_003935"
     CDS             complement(545..1168)
                     /locus_tag="JN691_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7245211.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(1165..1353)
                     /locus_tag="JN691_003936"
     CDS             complement(1165..1353)
                     /locus_tag="JN691_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HAY7245212.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            complement(1350..1712)
                     /locus_tag="JN691_003937"
     CDS             complement(1350..1712)
                     /locus_tag="JN691_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7245213.1"
                     /translation="MNEYQFVLPYPPSLNTYWRRRGSRYYISDKGQKYRKDVQQIIRQ
                     LKLDIFTKARLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENV"
     gene            complement(1709..1999)
                     /locus_tag="JN691_003938"
     CDS             complement(1709..1999)
                     /locus_tag="JN691_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY7245214.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKP"
     gene            complement(1999..2769)
                     /locus_tag="JN691_003939"
     CDS             complement(1999..2769)
                     /locus_tag="JN691_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HAY7245215.1"
                     /translation="MNAVIIANAEIVPIEFNGERVVTYQMIADIHGVPVATIQNGYAR
                     NSEYFIDREDTYFIDFHENTFRRGFEIPPRGMRVFTESGYLMLVKTLRDETAWRVQRE
                     LVRNYFRGQKEDKSALPDFTNPAEAARAWANEYEQKVKLEQQLAIAAPKVEFADRVGE
                     ASGILIGNFAKVVGIGPNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVN
                     TNHGIQISFTTKITGRGQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(2784..3020)
                     /locus_tag="JN691_003940"
                     /pseudo
     CDS             complement(2784..3020)
                     /locus_tag="JN691_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3095..3775)
                     /locus_tag="JN691_003941"
     CDS             complement(3095..3775)
                     /locus_tag="JN691_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HAY7245216.1"
                     /translation="MATQISVETLSPITHNQIPVITTELLAQLYCTEINNIKVNYTRN
                     SERFVEGKHFFKVVGDELKNLRVTLSNSQTPVSPKTRSLILWTERGAARHAKMLETDQ
                     AWDVFEKLEDCYFSQTSKSAGHHEKKTSELSAKEANSLVWLWDYAHRSQALFRELYPA
                     LRQIQSNYSGRCHDYGHEFSYVIGMARDVLINHTRDVDINEPDGPTNLSAWVRLKNKE
                     LPPSLHHY"
     gene            4097..4426
                     /locus_tag="JN691_003942"
     CDS             4097..4426
                     /locus_tag="JN691_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY7245217.1"
                     /translation="MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            4416..4724
                     /locus_tag="JN691_003943"
     CDS             4416..4724
                     /locus_tag="JN691_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005286394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7245218.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4766..4942)
                     /locus_tag="JN691_003944"
     CDS             complement(4766..4942)
                     /locus_tag="JN691_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HAY7245219.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKPKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4939..>5088)
                     /locus_tag="JN691_003945"
     CDS             complement(4939..>5088)
                     /locus_tag="JN691_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HAY7245220.1"
                     /translation="DVLPGLEGECVNIRESSAQMSVKRMASLIEYTTAWAIGQGVRFT
                     DRRYE"
BASE COUNT         1221 a         1273 c         1200 g         1394 t
ORIGIN      
        1 catggatttc atagcctcac ctccgcaaat aacggatggc gtagttctta tattgggaag
       61 gggaaaagaa ggccgcagcg taactgtcac tgatgaattc aggatagcca gtggctacgg
      121 ctcagttatg gtgctggtta acggacttga accgctaccc attcgcttac aaggcgactg
      181 ctctaccatt agagctaaac cagcatattt ggcgggacag cgtggactcg aaccacgata
      241 agaaggttaa cagccttccg taatgacctt tatacgactg acccaaataa aaaatcccga
      301 aagcaggctc tattcctatg tgctcccgag tttatgatgc gcatgtcagt gcgcttagcg
      361 aaaaacgccg taaaccctcg cccgacaggg cggggagcag tcacaactta aagatgagga
      421 gggtaaaaaa ccgaaagaat gtgaacggat ataaacctgc cattcttgag tcaaatttac
      481 ccaactttat tcaaaaagtc aatatcatgc cgttaatatg ttgccatccg tggcaatcat
      541 gctgctaacg tgtgaccgca ttcaaaatgt tgtctgcgat tgactcttct ttgtggcatt
      601 gcaccaccag ggcgtcatac agcggcttaa cagtgcgtga ccaggtgggt tgagtaaggt
      661 ttgggattag catcgttaca gcgcgatatg ctgcgcttgc tggcatcctt gaataaccga
      721 cgcctttaca tcttccgcac tctttctcaa caactctccc ccactgctct gtttttgcta
      781 tatcaactgc ccggcctgta ccgtggcaat ctctgcatct tgcgcccggc gtcgcggcac
      841 tacgacaata atccgcataa gcgaatgttg cgagcacttg cagtaccttt gccttagtgt
      901 ttccttcgag ctttgccaca ccacggtatt tccccgatac cttgtgtgca aattgcatca
      961 gatagttgat agccttttgt ttgtcgttct ggctgagttc atgcttaccg caaaatgcag
     1021 ccattccgaa tccggcttgt gattgcgcca tccccatagc agccatcaca tcagtaccgg
     1081 aaagagagtc agaagccgta gcccgtggtg agtcgctcat catcgggctt tttggcgaat
     1141 gaaatttagc tacgctttcg agtctcatcg tcttcccctc ttgccctgtt tgaccatcag
     1201 gacgccgtta actattacgt gacgctcgcc tttgctgtct cggttgtact tgagcactgt
     1261 tcctcttgcg caggaaagca tcctcgccac ttcggtctga ttgcctcgtg tctggataag
     1321 aagctctggt atcgtttgaa ttgtggcgtt catacgttct ccagttcggt gatttttatt
     1381 ccaagccttc cgcctggtac tttcacacca cgaattacgc gaatgtcatc gaattgctcg
     1441 tcgtcttccg caaatccggc gtggataaga gagtcgagta gccccttcag gatgttatcg
     1501 aggtcgcggc ggcgggagtc tggaacgtct gcgatgactt tgatgcggag tcgtgctttg
     1561 gtgaaaatat ctaacttgag ttggcggatg atttgctgaa cgtcttttcg gtatttctgg
     1621 cctttatcgc tgatgtagta tcggcttccc cgtcttcgcc agtaggtgtt cagcgacggc
     1681 gggtatggaa gcacaaactg atattcgttc atggcttaat cttcccctcc ttcagcagta
     1741 tcgcctgcgt cctgatcacg ccttcgaggt ggtaaagcct ggcgtctttg ttgtcgagat
     1801 tatgggtgcg tcggtcgatt tcatcgtgac acgcgctaca agcccatgcg ccgatcaggt
     1861 cgtcaggctt cattcccgtt ccgcaaattc cagccatccg gtaatgtgcc agaactgtag
     1921 tttcagggtt gccattgcat acgccgtaaa tacgtacctg gcattctctg ccgcgtgctt
     1981 ctttgcgtag gttagccatt aagcagcctc cccggttact ttcagcattc cgttatcgag
     2041 cagctttctg gtcagccact gttgaccacg cccggtgatt tttgtggtga acgatatctg
     2101 tattccgtga tttgtgttga ccgctgtttc tttcactgtg aaatagccgc gatccatata
     2161 ttcctgcatt ggcacattgc gccgggaacc tgaagcaata aggattttgt gatcacgcat
     2221 ccacgcaaac agtttgtttg ggccaatacc aacaaccttt gcaaagtttc caatcaaaat
     2281 tccgctggcc tcgccaacgc gatcggcaaa ctcaacttta ggtgcggcaa tcgcgagttg
     2341 ttgctccagc ttcactttct gctcatattc gtttgcccac gctctggcgg cttcagcagg
     2401 gtttgtgaag tcgggcaagg ctgacttatc ttctttttgg ccacgaaaat aattgcgaac
     2461 aagctcacgt tgcactctcc acgccgtctc gtcgcgcagg gttttaacca gcatcaaata
     2521 gcctgattca gtgaaaactc gcatgccacg aggaggaatt tcaaaacccc ttctaaaggt
     2581 attttcatga aaatcaataa aatatgtatc ttccctgtct atgaaatact cagagtttcg
     2641 cgcatagcca ttttgaatag tggctactgg aaccccgtga atatcagcaa tcatttgata
     2701 cgtaacaaca cgctcgccat taaactcaat cggcacgatt tctgcgttgg caataattac
     2761 ggcattcatg caacacctcc ttcaacgaca ataatgctgt cgcgcttacc ttgttcgcct
     2821 tcgaagacgt aatgctcgta ctgaacattg aaccctaagt tattgattct ttcggaaacc
     2881 tcaaattgag gatgccggat aacaccattt ttagccagcg tttcgatggt acgtttcaca
     2941 ttgtcatgac gcttacccac caactcagcg atttcaatgc tggtcatttt gatggcgttg
     3001 ctatttatca gctcgttcat tgtcatgtcc tctcatattg aaaattcagc aataaaaaac
     3061 ccagccgaag ctgggtcgtt gcgttggcaa tctgtcagta gtgatgtagt gaaggaggta
     3121 attctttgtt cttaagtctt acccatgcag aaagattcgt tggtccgtct ggctcattaa
     3181 tatcaacatc tcgtgtgtga ttgattaaaa cgtctctcgc cattccgata acatacgaga
     3241 actcatgacc gtagtcgtgg catctgccgg aatagttaga ctgaatttgt cttagcgccg
     3301 gatacagttc gcggaataat gcctgtgagc ggtgggcata atcccacaac catacaaggc
     3361 tgtttgcttc ttttgcagaa agctcgctgg ttttcttctc gtgatgacct gcgcttttcg
     3421 aagtctggct gaaatagcag tcttccagtt tttcgaacac gtcccatgcc tgatcggttt
     3481 cgagcatttt tgcgtggcgg gctgctccgc gttctgtcca gaggatgagg gagcgggttt
     3541 tgggggaaac aggcgtttgt gagttactta aagtaacccg caaatttttc aactcatcac
     3601 caacaacctt gaaaaagtgt ttaccctcaa caaaacgctc ggaattacgg gtgtaattta
     3661 ctttgatgtt gttgatctcg gtacagtaaa gctgtgccag taactcggta gtgataacgg
     3721 gaatttggtt gtgtgtaatt ggggaaagag tttcgacaga aatctgagtg gccataatga
     3781 cgccctccgg tgattgtttt gtttatcacc accgccgacg ccaatcggat tgggtggtga
     3841 gacgtacagg gttggcgtaa ccggatcacc gaccggcgag cctttcggct cccccatacg
     3901 ccccaccata attcagatgc gcgtatacaa acgacaataa aaaacacgct cgcggcgtgt
     3961 ctctgtcgcg gtgaaattcc gggacgccaa tcccgacgcc agattttgct ggcgtatgag
     4021 gaatatagcc ccggatatgt gttgtcgtca actacaggcc ttgaatgcat ataacgattt
     4081 tgttatgttg atacctatga actacactat cgaatactac agtgaagatg taaggctgga
     4141 agtcgatcgg cttccattga gtatgcgtgc ccgataccaa catctcgttg aacgtatgaa
     4201 gatatatggc agcaatctcg gagaacctca caccagcgcc tttggtgacg ggcttttcga
     4261 actcagaatt aaaggcaacg atgggatcgc gcgtgttttt tactgcactc tgacagggaa
     4321 acgcatcatc atgctgcata gttttgtaaa gaaaacgcag aaaacaccgc cagccgaacg
     4381 caagaaagct gaaaccagaa tgaaggaggt taagcatgac tggtaaacgc actctcccca
     4441 ccatgacaca cgacgaaatg gcagccaaat ggatggaaga cccggctttt aaagcagaat
     4501 acgacgctat cgctgacgaa ttcgcactgc ttgatgaaat gctggcagca cgcaaagaag
     4561 ctggcttaac tcaggctgaa gttgccgagc gaatgggaac aaaagcgacc gcgatcacca
     4621 gaatggaaag taatctcgca tcaggtatca gcggcccatc atttgccaca ctaaaaaaat
     4681 tcgcccgcgc tactggaaaa aaactccaga tccgcttcgt ttaaccacac cgcgccgtca
     4741 ttctggcggc gcaccggata aatagtcatt tcctcgcacg atgtcttagc caccggatat
     4801 cccacaggtg agccgtgtag ttgaaggttt ttacgtcaga ttcttttggg attggcttgg
     4861 gtttatttct ggagcgtttc gttggaaggt atttgcagtt ttcgcagatg atgtcggtga
     4921 tacttcgtcg ctgtcgtctc attcgtacct cctgtcggta aatctgacac cctgaccaat
     4981 agcccatgct gttgtgtact cgatcagact tgccatgcgc ttcacgctca tctgcgcgct
     5041 gctttcgcga atgttgacgc attctccttc aaggccgggc aaaacatc
//
LOCUS       DADLSA010000221         5036 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.222, whole genome shotgun sequence.
ACCESSION   DADLSA010000221 DADLSA010000000
VERSION     DADLSA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.222"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..207)
                     /locus_tag="JN691_003946"
     CDS             complement(<1..207)
                     /locus_tag="JN691_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HAY7245221.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAAFSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKP"
     gene            complement(316..729)
                     /gene="hiuH"
                     /locus_tag="JN691_003947"
     CDS             complement(316..729)
                     /gene="hiuH"
                     /locus_tag="JN691_003947"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY7245222.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            862..1533
                     /gene="hprR"
                     /locus_tag="JN691_003948"
     CDS             862..1533
                     /gene="hprR"
                     /locus_tag="JN691_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAY7245223.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1533..2891
                     /locus_tag="JN691_003949"
     CDS             1533..2891
                     /locus_tag="JN691_003949"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HAY7245224.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2999..3850)
                     /gene="hchA"
                     /locus_tag="JN691_003950"
     CDS             complement(2999..3850)
                     /gene="hchA"
                     /locus_tag="JN691_003950"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HAY7245225.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(4444..4653)
                     /locus_tag="JN691_003951"
     CDS             complement(4444..4653)
                     /locus_tag="JN691_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245226.1"
                     /translation="MSGDSGGQSTGYKDSDRLNYIEIGTWYYFNKNMNIYTAYQINLL
                     DKSDYVFAHGLNTDDQLAVGIVYQF"
     gene            4717..>5036
                     /locus_tag="JN691_003952"
     CDS             4717..>5036
                     /locus_tag="JN691_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7245227.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELADAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRA"
BASE COUNT         1419 a          996 c         1190 g         1431 t
ORIGIN      
        1 aggcttgctg aactccagcg gttttccggc gggggcgggc ggacgatcgt tccctttaaa
       61 ccagctaagc agatcggcat gcgcagcgtg aggcaaagaa aatgcagctg cgctgatgcc
      121 cagtgctttt aacacctgcc gacgcttcat aaagaatacc gactcggccg taacatctga
      181 ttcttttaaa aattgattct ttttcataag ccatcacact tttttgtcgt taaccaacaa
      241 gagctgttta ctgttaatct gtcagggagt cacctcccta acagtagcag agatcagctt
      301 ttacatattt acgcattaac tgccacgata ggttgaatac ccatattggc tgagtaacaa
      361 aggcacatga taatgctcgt tcactttatt aatatgaaat tcaacgggaa tctcagggaa
      421 gaaactttca agattttgtt tcttgaaata gtccccggtt ttaaatacga cacggtaatc
      481 gccagtagtc gcagtttgct cgggccacag tgccttaatt cgtccatcct tatctgtttt
      541 ggcggtatta agttgtaacc agccgttatc cgctttcttt tcaagagtga ctgtcacgtc
      601 ggcagcgggt tttcctgttt gctggttcaa aatgtgcacg ctaagaatgt tttgttgtgc
      661 ggcataaacc aaagaaggta atgaaaatgc tgccgttgct accgagagta ctaaataacg
      721 ctttaacatg tttatatcct tgtcatgtga atgagttttg aggatagcga aaaaacgtcc
      781 agggtaacct tacagcagca ttacaatttt gtaatgaacc aggttgtttc tataacatat
      841 gatttatggc atattatttt catgaagatt ctacttattg aagataatca aaggacccag
      901 gaatgggtaa cgcaggggct ttccgaagcg ggttatgtca ttgatgccgt ttctgatggc
      961 agagatgggc tttatcttgc gctgaaggat gattatgcat tgatcattct ggatattatg
     1021 cttccgggta tggatggctg gcagatctta caaacgttaa gaacggcaaa gcaaacccct
     1081 gttatttgcc ttactgcaag ggattctgtc gatgacagag tcagagggtt ggacagtggg
     1141 gcaaatgatt atctggtaaa acctttttca ttttctgagt tgctagcaag ggttcgggca
     1201 caattaaggc aacatcacgc tctgaattca acattagaaa tcagtggctt aaaaatggac
     1261 tctgtcagtc aaagtgtgag cagggacaat atcagtatta cactgacgcg caaggagttt
     1321 cagttacttt ggctactggc ctccagagct ggcgagatta tacccagaac ggttattgcg
     1381 agtgaaattt ggggaatcaa ctttgatagt gataccaata cggtggatgt cgccattcgc
     1441 aggctccgcg caaaagttga tgatcctttt cctgaaaagc taatcgccac aatccggggg
     1501 atgggctatt cattcgtagc ggtaaaaaaa taatgaaaag attatctata accgtccgtt
     1561 taaccttgct ttttatattt ctgcagtctg ttgctggcgc tggaattgta tggactctct
     1621 ataatggcct ggcaagtgag ttgaaatggc gcgatgatac aacactcatt aaccggacag
     1681 cgcagatcaa gcagttgtta attgatgggg taaatccaga tacgttacct gtgtacttta
     1741 accggatgat ggatgttagt caggatattt tgatcattca tagtgatggc atcaataaaa
     1801 ttgttaaccg gacaaatgtc agtgatgaca tgttaaataa catacctgct agtgagacaa
     1861 tcagcgcagc aggcatttac agaagcatta ttaatgatac agagatagat gctttacgaa
     1921 ttaacattga tgaagtttcg ccatcattaa cggttactgt ggctaaattg gcttcagcca
     1981 gacataatat gcttgaacag tataaaatta atagcattat aatttgcatt gtcgccatta
     2041 tactgtgctc agtattaagt ccgttgttaa tcaggacggg gttgcgagag atcaaaaagt
     2101 tgagtggcgt aacggaagcg ctgaattata acgatagccg agtgccggtt gaggttaacg
     2161 cattaccgag agaactaaaa cctcttgggc aggcgttgaa taaaatgcat catgctttag
     2221 tcaaagattt tgagcgcctt agtcaatttg ctgacgatct cgctcatgaa cttagaacgc
     2281 caattaatgc attactgggt cagaatcagg ttacgctcag tcaaaccaga agtatcgctg
     2341 aatatcaaaa aacaattgcc ggaaacattg aagagctgga aaatatttcg cggttaacag
     2401 agaatatact gtttcttgcc agggcagaca aaaacaatgt tttggtgaaa ctggattcgc
     2461 tttctctcaa taaggaagtt gaaaatcttt tggactatct tgaatatctt tcagacgaga
     2521 aagagatttg ctttaaggtc aagtgcaatc aacaaatctt tgcggataaa attttactgc
     2581 aacgaatgtt atcgaatctt attgttaacg ccattcgata ttcaccagaa aaatcgcgta
     2641 ttcatataac cagttttctt gatgccaacg gctctcttaa tattgatatc gccagtcctg
     2701 gaacgaaaat taatgagcct gaaaaactct tccgtagatt ttggcgggga gataattcgc
     2761 gtcattccgt aggtcaggga cttggccttt ctttagtcaa agcgattgcc gaattacatg
     2821 gaggaagtgc tacgtatcac tatctcagta agcataacgt gttccgaatt acgttaccgc
     2881 aaagaaatta acatcgggct tcagtacgcc ataagttaac cgtgaaaaaa cgcagccacg
     2941 tcgtgagtat taacgtggct gcgattgatt atgcgcttac atcacaatgt aacagggatt
     3001 aactcgcgta agctgccagc atttcctgcg ccgccagttt acccaacgca ttcgctgcaa
     3061 aaggactgtc gccggtgaga agtttacggt ccttatgtac tcgcccggtg atgtcgtcat
     3121 taatgatgtt catgcccatt ttcttcaact cttcgccgaa gtaccaggtg agatgacccg
     3181 gcatatagcc aatatctggc gtttgtttgt ctgcggcgtc tgggaatgcg caaatagaat
     3241 aaccatttag tgggttatcg ctgtggcgaa gtgccagaaa agccgccgga ccgtggcaaa
     3301 gggagataac aaaacggtca tttttgattg cccactgtaa agcggcagcc acgtcctggc
     3361 tttctggtaa accaataagt gcgccatgcc caccaggaac aaagattgct gcatattcgc
     3421 tatcagcgtt gaggctggcg acaacatccg cgagtttctt cggattacgg aataacgatt
     3481 tatgctgttc aaagaatggc attacttttt catctttgtg cggcatagcc cagtattcaa
     3541 acttggtcat cagaccggaa atggtcgcca cttcgaattc gaaacctgca gcatggagat
     3601 gatacaacgg cagcaacgtt tcaatcggat ggttaccggt cgagaacagt tttccgttat
     3661 cggtcggcaa ataacgttcg tccgcggcga tcaccagaat tttatgttta ccgcgatacg
     3721 gttttggata gtccacgcca tcaagatcag agacgggact cgtatattgg ctaagcgaat
     3781 attctgaagg gaagaacgca ttatcttcag caatatcgac ctgcgggttt ttacttgttt
     3841 gaacagtcat agtgtattcc ttattgttgc ttagttaggg tagtcactat atttgcgact
     3901 gagctggtcg ctatttttaa ccaggctgaa aaattgagtc aatatatcga aagcggtagt
     3961 tggcgggcat aagtatgtaa tgatgatgct gcactggtgt tgaatattat tcacgccaaa
     4021 ctttcctcat tgagcaatgt cgtcgcaagt ggcctcaatg attttagacc attatcagag
     4081 gtccggagcg gcgggaagag atttattgct gtggaataat tgtggtactg atgattattt
     4141 atatcccgga ttggtaggga aaagcttttt tcatgcacta tttttgcaaa tatttctata
     4201 tgggaagcgg ttttgttaca gattaaattt ataaagaaat gcatttgggg tgttgggttg
     4261 aagagtagct ggtagatgtt attgtgccag caggggcagg ggaatgtgcc gtatcgatat
     4321 gctttggcaa ttgcgtgtgc cgcagatgga gatgaaatgc caataaaaaa gcctgagttc
     4381 gatgagggac gaactcaggc tggtgttatg ttttacagta acacgcctcg gcgcgttggc
     4441 tgattagaac tggtagacga taccaactgc cagttggtcg tcggtgttca gtccgtgagc
     4501 gaaaacataa tcactcttat caagcaggtt gatctgataa gcggtatata tgttcatatt
     4561 cttattgaag taataccagg taccgatttc aatatagttc agacgatcag aatctttgta
     4621 acctgtagac tggccccctg aatctccaga caaccaatat cacttattta agtgatagtc
     4681 ttaatactag tttttagact agtcattgga gagcagatga ttgatgtctt aggaccggag
     4741 aaacgcagac ggcgtactac acaggaaaag atcgctattg ttcagcagag ttttgaaccg
     4801 ggaatgacgg tctcccttgt tgcccggcaa cacggcgtgg cagccagcca gttatttctc
     4861 tggcgcaagc aataccagga gggaagtctt actgctgtgg ccgcaggaga acaggtcgtt
     4921 cctgcctctg aacttgctga tgccatgaag cagattaaag aactccagcg cctgctcggc
     4981 aaaaaaacga tggaaaatga actccttaaa gaagccgttg aatatgggcg tgcaaa
//
LOCUS       DADLSA010000222         4997 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.223, whole genome shotgun sequence.
ACCESSION   DADLSA010000222 DADLSA010000000
VERSION     DADLSA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.223"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="JN691_003953"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JN691_003953"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="JN691_003954"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="JN691_003954"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAY7245228.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="JN691_003955"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="JN691_003955"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY7245229.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADCNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="JN691_003956"
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="JN691_003956"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HAY7245230.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADVRRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(3361..4695)
                     /locus_tag="JN691_003957"
     CDS             complement(3361..4695)
                     /locus_tag="JN691_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7245231.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1207 a         1418 c         1186 g         1186 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 cgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacgcc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacatcag caatagcttc tgggttacag tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcaca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt accggctcgt tagtcggcac
     3121 tcgcactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aaccaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaatg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccagg ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 agcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatccctg aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacc
//
LOCUS       DADLSA010000223         4926 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.224, whole genome shotgun sequence.
ACCESSION   DADLSA010000223 DADLSA010000000
VERSION     DADLSA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.224"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(99..1838)
                     /gene="flhA"
                     /locus_tag="JN691_003958"
     CDS             complement(99..1838)
                     /gene="flhA"
                     /locus_tag="JN691_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HAY7245232.1"
                     /translation="MLSRSDLLTLLTINFIVVTKEAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLSGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRH
                     QLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDGVL
                     GNDPAYGMLVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSTVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEV
                     PDELELKIMGALM"
     gene            1783..2568
                     /gene="lafU"
                     /locus_tag="JN691_003959"
     CDS             1783..2568
                     /gene="lafU"
                     /locus_tag="JN691_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAY7245233.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQRLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            2639..3694
                     /gene="dinB"
                     /locus_tag="JN691_003960"
     CDS             2639..3694
                     /gene="dinB"
                     /locus_tag="JN691_003960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAY7245234.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            3746..4039
                     /gene="yafN"
                     /locus_tag="JN691_003961"
     CDS             3746..4039
                     /gene="yafN"
                     /locus_tag="JN691_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HAY7245235.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            4042..4440
                     /gene="yafO"
                     /locus_tag="JN691_003962"
     CDS             4042..4440
                     /gene="yafO"
                     /locus_tag="JN691_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HAY7245236.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            4450..4902
                     /locus_tag="JN691_003963"
     CDS             4450..4902
                     /locus_tag="JN691_003963"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7245237.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
BASE COUNT         1202 a         1267 c         1307 g         1150 t
ORIGIN      
        1 gctggcgcgt cttatcatgc ctacaaactt gtgcctgaac ggtaggccgg ataaggcgtt
       61 tacgccgcat ccggcaattg tgcaccgatg ccggattctt acatcaacgc ccccataatc
      121 ttcaactcca gctcatccgg cacttcgtta tacgacagca catgcagccc cggcgcaaac
      181 aaccttgcat aacgcgccag caaagggcgt agctgcggcg gcaccagcag caccggatct
      241 ttccccgccg ctttcatctg ctctttcacc tgtggcatcg tgctctggaa ctggttgagc
      301 atattcgggt caaccggcac gctgtcgagc atcactttgc cggcctgttg cgcctgattc
      361 actacgttgg tcagcagatt ttccagctca ttattcagcg tatacaccgt cagctcctgc
      421 ttgcgaacga acggatgagt aatgctgcgc cgcagcgcca ggcgcacatc ggccgccagc
      481 agaatatgat ctttagtcac cgtgctactg gcgaccagca cggtggcgat ggtgacgata
      541 tcgcgcaggg aaacgccttc ggtcagcagc gcccggtaca ctttcagcaa ttggctgtaa
      601 ttgagcgccg cgcttaaatc ctccgccagg cgcggtgcca tcgacgacaa acggttatgc
      661 aactgcgtga tgtcatcata gttaaacaaa tcaggaatat agctgcgcac aatcttgttc
      721 acatgggtgg caatcacact ggcgctgtcg atcacctgat accccatatt cagagccttc
      781 gctttctgcg caggctggat ccaggtgacc agcatcccgt acgccgggtc gttccccagc
      841 acgccgtcaa tctcgccgta ggtttcgctg gagggcagcg ccatcagttt atccgccgga
      901 atatccgctt catcagcctt aatgccgttg atgaaaatag cgtactggct gggcttgagg
      961 cggaagtttt cccgaatgcg gatctccggc agcagcacgc cgttaccgtc ggaaatcacc
     1021 tgccgaacgc cgcgaatccg ctgggtgagc gggttgccct gcgctttgtc caccagcgcc
     1081 accagcttgt agccgaggct taagctgatg ggctcgatca gcggaatagt ttcccaactg
     1141 acctgttgtt cgctggtttc agtgatggtg cgggtcagcg tttcgaggct tttctcttcc
     1201 gcctccgccg cctgcgggcg tttgctcatc cgccagccgg taaagccaag cagggcgctg
     1261 aacagcaaaa acggcagatg cggcatcccc ggcaccaccg ccagcacgaa catgatcccg
     1321 gtggcggtgt agagcaccga cgggcttgcc agcaactggt ggcgcacgtc atgggcgata
     1381 tcgccgttgt cgctgacgcg ggtgacgata atcgccgccg cggtggagag cagcagggaa
     1441 gggatctgcg ccaccaggcc gtcgccgatg gtcatcagca cgtattgctg gaaggcggcg
     1501 tcggcgctca ggttgtattt gaagatgccg atacagacgc cgccgctcag gttgatcgcc
     1561 agaatcatca tcccggcgat ggcgtctccg cgcacaaact tcgatgcccc gtccatcgcg
     1621 ccgtagaaat cggcctcgct ggcaacatct ttacgccggg tttgcgcctg cgcctggttg
     1681 atcaatccgg cgttaagatc ggcgtcaatc gccatctgtt tgccgggcat cgcgtccagg
     1741 gtaaagcggg cagaaacctc ggaaatacgc tcggcctctt tggtgacgac gataaagttg
     1801 attgtcaaca gcgtcagtaa atccgagcgt gaaagcatta tcgccgcgct gcacgggcag
     1861 tcgattttta acggtggtgg gctgtcgccg ttaaataaaa tcagcccgtc tcatccgcca
     1921 aaacctgcca ccgtggcggt gccggaagag acggagaaaa aagcgcgtga tgtgaatgag
     1981 aaaacggcgc tgctgaagaa gaaatccgcc accgagcttg gcgagttggc aaccagtatc
     2041 aacaccattg cccgtgatgc gcatatggaa gcgaatctgg agatggagat tgttccccag
     2101 agattacgcg tgctgattaa agacgaccag aatcgcaata tgttcgaacg cggcagcgcg
     2161 cagattatgc cgttctttaa aactttgctg gtggagctcg cgccagtgtt cgactcgctc
     2221 gataacaaaa ttattattac cgggcatacc gatgcgatgg cctacaaaaa caatatctac
     2281 aacaactgga acctttcggg cgatcgcgcg ctttcagctc gtcgggtgct ggaagaggcc
     2341 ggaatgccgg aagataaagt gatgcaggta agcgcaatgg cggaccagat gctgctggac
     2401 gcgaagaatc cgcaaagcgc gggcaatcgg cgtattgaga ttatggtgct gaccaaaagt
     2461 gcgtccgata cgctgtatca atactttggt cagcatgggg ataaagtggt gcagccgctg
     2521 gtgcaaaagc tggataagca gcaggtgctt tcgcagcgaa tgcgctaaat gctgaatctt
     2581 tacgcatttc tcaaaacctg aaatcactgt atactttacc agtgttgaga ggtgagcaat
     2641 gcgtaaaatc attcatgtgg atatggactg ctttttcgca gcggtggaga tgcgcgacaa
     2701 tcccgccctg cgcgatatcc ctattgctat tggcggcagc cgcgaacgtc ggggggtgat
     2761 cagcaccgcc aattatcccg cgcgtaaatt tggcgtacgt agcgctatgc ctactgggat
     2821 ggcgctcaaa ttatgcccac atctcacctt gcttccgggg cgctttgacg cctacaaaga
     2881 agcctcaaat catatccgtg aaatcttctc gcgctacacc tcgcgcattg aaccgttgtc
     2941 actggatgag gcttatctcg atgtcaccga tagcgtccat tgccacggtt ctgcgaccct
     3001 catcgcccag gaaatccgcc agacgatctt caacgagctg caactgacgg catctgcggg
     3061 cgtggcacca gtaaagtttc tcgccaaaat cgcctccgac atgaataaac ccaacggtca
     3121 gtttgtgatt acgccggcag aagttccggc atttttacaa accttaccac tggaaaaaat
     3181 ccccggcgtc ggcaaagtct cggcggcaaa actggaagcg atggggctgc ggacctgcgg
     3241 tgatgtacaa aagtgtgatc tggtgattct gcttaaacgc tttggcaaat ttggccgcat
     3301 tttgtgggag cgtagtcagg ggattgacga gcgcgacgtt aacagcgaac ggttacgaaa
     3361 atccgtcggc gtggaacgca cgatggcgga agatattcat cactggtctg aatgtgaagc
     3421 gattatcgag cggctgtatc cggaacttga acgccgtctg gcaaaggtaa aacctgattt
     3481 actgattgct cgccaggggg tgaaattaaa gttcgacgat tttcagcaga ccacccagga
     3541 gcacgtctgg ccgcggctga ataaagctga cttaatcgcc accgcgcgta aaacctggga
     3601 tgaacgccgc ggcgggcgcg gtgtgcgtct ggtggggctg catgtgacgt tgcttgaccc
     3661 gcaaatggaa agacaactgg tgctgggatt atgatgtata ctattatgta tattctggtg
     3721 tgcattatta tgagggtatc actgtatgca tcgaattctc gctgaaaaat cggtcaatat
     3781 cactgagtta cgtaaaaacc cagctaaata ctttattgat caaccagttg cggttctttc
     3841 taataatcgc cccgcaggat atctcttaag tgccagcgca ttcgaagcgt taatggacat
     3901 gcttgctgaa caagaggaga aaaagcccat aaaggcgcgc tttcgtccaa gtgctgcaag
     3961 attagaggaa attacacgcc gcgctaaaca atatcttaat gatatgacgg atgatgattt
     4021 caatgacttt aaggaataag gatgcgggta ttcaaaacaa aacttattcg cctgcaactt
     4081 acagcagagg aacttgatgc gttaacggcg gattttattt cctataagcg tgacggtgtt
     4141 ttgccagata tatttggtcg cgatgcactc tacgacgact cgtttacctg gccattaatc
     4201 aaatttgagc gagttgctca tattcatctg gcaaatgtga ataatccatt tccgccacag
     4261 ttgcgccaat tcagcagaac gaatgacgaa gcgcatttgg tatattgtca gggtgcgttt
     4321 gatgagcaag catggttgct cattgccatt ctgaagcctg aacctcataa actggctcga
     4381 gataacaacc aaatgcataa aattggaaaa atggcagaag cgtttcgcat gcgtttttga
     4441 atttatatta tgaataacat acaaataaga gattatcagc ctggcgattt tcagcaacta
     4501 tgcgctattt tcattagagc ggttacgatg accgtcagtc agcattattc accacaacaa
     4561 attgccgcct gggcgcagat tgacgaatct cgctggaagg agaaactcgc gaaatcacaa
     4621 gtgcgggttg cagtcattaa tgcaaaactg gtcggcttta ttacctgcgt tgaacactat
     4681 atcgatatgt tatttgttga ccctgaatac acccgccgtg gggttgccag cgctttgtta
     4741 aaacctttga ttaagtctga atccgaactt acggtggacg caagcataac cgcaaaaccc
     4801 ttttttgaac gttatggttt tcagacagtt aagcagcagc gcgttgaatg ccggggagcg
     4861 tggtttatta atttttatat gcgttataaa ccgcaacatt aaatccagct tgcaatgaaa
     4921 ataacg
//
LOCUS       DADLSA010000224         4901 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.225, whole genome shotgun sequence.
ACCESSION   DADLSA010000224 DADLSA010000000
VERSION     DADLSA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.225"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            524..1087
                     /locus_tag="JN691_003964"
     CDS             524..1087
                     /locus_tag="JN691_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004938731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7245238.1"
                     /translation="MKVSKIALAMGLSMALVSGFANAADSGTTPPANTAAEGGHGHGT
                     VHFKGEIIDAPCSISPESADQTVDLGQVANSALKDKGTSSPVAFNIKLQNCDISTYKT
                     VTATWSGTPDVNMPEAWGITGTASGAAIVLRDASEKEITLGQDTAPTTLTADDTTIAM
                     SAFLKGDGEAIVPGAFTGAADFVLSYQ"
     gene            1163..1684
                     /locus_tag="JN691_003965"
     CDS             1163..1684
                     /locus_tag="JN691_003965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7245239.1"
                     /translation="MLRIVFLLLLLVSSTGRAVSHGHGHVQMQGTIIDTACAIATGDV
                     EQGIDFGALTASELINNGRGPSLPFTVHLVNCVLDGTDIRGLNHWKDVRITFVGDADS
                     ENHFALQGDGRGAALVIADSQGVEAKPGEPMPSTPITPGSMALHYQLWLTGGKTIRPG
                     HIHTTVRYFMEYD"
     gene            1684..4281
                     /locus_tag="JN691_003966"
     CDS             1684..4281
                     /locus_tag="JN691_003966"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012786.1,HMM:NF025319.1,HMM:NF025320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY7245240.1"
                     /translation="MFNQKSGNNGGLNNVRLNSLTWMLLLVLGGIPAAQSQDNMSTPA
                     NSNIPDEVVEQPNITEFNTDVMDVNDRNNIDVSHFSRAGYVMPGEYPLILVVNGTEIR
                     EVTVSFAPPEDDPQGSSPCLTPEMVKLFGLKDEATKKLIWKGNGDDRCLDEKSLPGMT
                     ATGDLSTGSLKISLPQAWLEYSAPDWDPPSRGDNGVADLLFDYNLNASKSHALSEGGS
                     SATSLSGSGTAGANFGPWRLRADWQASRYSDRGYTSQSMDWSRFYLYRALPKLGARLT
                     LGESDLDSSLFDSFRFIGGSLVTDDNMLPPNLQGYAPEVTGVARSNAKVVISQQGRVI
                     YESQVAAGPFRIQELSSSVSGTLDVRVEEQDGRVQHFQVDTASIPYLTRPGLVRYKAS
                     LGKPTDWKHHVQDVSFATGEFSWGMTNGWSLFGGLVAAKKYAALAVGIGRDLLSFGAV
                     SFDVTESKATLDTGEKKMGGSYRLSYSKRFEKIDGQITFAGYRFSEKDFMSMNDFLET
                     EYDAGSRSDTAWGRQKELYTITVSKAFPDWNMNLYGNYSHRTYWNRPDADRYSLSLSK
                     YMDVGRWKGISLNLTGYRSEAWGVKDDGVYASLSIPWSDGGSLSFNGQSTTAGTTQMV
                     GWYDRIDSNNNYSLQAGTSVGNKASASGYVSHNGDLTNGTLSASYYEGNNTSLGLSLQ
                     GGMTVTRYGAALHPGAMNGGTRLMIDSGDVAGVPLSHSGVLQRTNAFGVGVIGNLNSY
                     WRNSVGVDVNALDDDMEVSHPLTELTMTESAIGYRKIDVLEGRKLMAVVKFADGSSPA
                     FGATVWRGSHQTGVVDDNGSVWLAGVKPGVTMEVREDENPVCQVGIPERLPPENTDQS
                     LLLTCQQKS"
     gene            4395..>4901
                     /locus_tag="JN691_003967"
     CDS             4395..>4901
                     /locus_tag="JN691_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015376357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7245241.1"
                     /translation="MNRNKSIKSQPFLKSLLVMALVSQSAFQVADAAVALDRTRVIFE
                     GGNKSMSLNIRNDNTKLPYLAQSWVEDEQGNKIQSPLMALPPLQRMEPGSKSLVKIQL
                     APGVEAKLPQDRESLFYFNLREIPPKSNKANVLQLALQTRVKMFYRPKALAKNQAMNE
                     TPWQEQLTL"
BASE COUNT         1292 a         1019 c         1301 g         1289 t
ORIGIN      
        1 ggacggtgaa agcaagaagc tattaggcgc tataggtctt ttgacggttt ttgaatttcc
       61 cctcctgata tctctgttat atttttgtga atttctccaa tccaaatcaa agtcccaata
      121 agtcatggat gcatggttaa aataactatc atttgtgtaa cctttggtgc ttataacttt
      181 tctttattct tttttcagga ttacctggag ggattgacca aaaaaacatt cattgaatga
      241 tatttttcta attaatgagg ttttctaagc tcgttgaaat aaaaaatata ttttaatcat
      301 gggtttgcat gcgtttcttt cgcctttttt acacaagttg acatcgaact cagcaactat
      361 aagaatagcc acatcaccag acggtgaccg ataaaggaaa taccgtttcc atatttaaat
      421 gaattggaaa atatcaccga tttgaagtcg actcgttcgg gtaattatta atactgaatc
      481 tttaatgctt atttcttaag cttgttaaca acgggaatga ataatgaaag tgtccaaaat
      541 tgcactggca atgggtctta gcatggcatt ggtaagcggc ttcgcaaacg ctgctgactc
      601 tggtacaact cctcctgcta atactgcggc agaaggcggc catggtcacg gcactgttca
      661 ctttaaaggt gaaatcattg atgcgccatg ctctatttct ccggaatctg cagaccagac
      721 ggttgatctt ggtcaggtcg ctaactctgc cctgaaagac aaaggtacgt cttctccggt
      781 cgctttcaac attaaactgc aaaactgcga catcagcact tacaaaactg taactgcaac
      841 ctggagtggt acacctgacg ttaacatgcc ggaggcatgg ggtatcactg gtacggcgag
      901 cggtgcagct attgttctgc gcgatgcgtc cgaaaaagag atcaccctgg gtcaagacac
      961 tgccccaact acgctgaccg ctgatgacac cactattgct atgtctgcgt tcctgaaagg
     1021 cgacggcgaa gcgattgtcc caggtgcgtt caccggtgcg gcagacttcg ttctttccta
     1081 ccagtaagta gccgcttaac tgtgctggct ccctggttag cagggagccg ttaagaacgt
     1141 gaaatcagac ggaagtatgg tgatgttgcg tattgttttc ctcctgcttt tgctggtgag
     1201 ttcgacaggg cgcgctgttt cacatggtca cgggcatgta caaatgcaag gaaccattat
     1261 tgatacagca tgtgccattg ctacaggaga tgtggagcag gggatagatt tcggcgcact
     1321 tacggcaagt gaattaatta ataatggccg tggtccttct ttgccattta ccgtccattt
     1381 agtgaactgc gttcttgatg ggacagatat tcgaggcctg aatcactgga aagacgtacg
     1441 tataacattt gtaggcgatg ctgatagcga gaaccatttt gctttacagg gagatggacg
     1501 tggcgcggct ctggtcattg ctgatagcca gggggttgag gcgaaacctg gagagcctat
     1561 gccttctaca cccattacac ctgggagtat ggcattgcat tatcaattat ggctaaccgg
     1621 cgggaaaaca atccggcctg ggcatattca taccaccgtt cgctacttca tggagtacga
     1681 ctgatgttta atcagaaatc cggaaataac ggcggattaa ataacgtcag attgaattcg
     1741 ttaacgtgga tgctgttgct cgtattgggt ggtattcctg ctgcgcagtc acaagataac
     1801 atgtcaacgc cagcgaattc gaatattcca gatgaggtag tcgagcagcc gaatataacc
     1861 gagttcaaca ccgatgtgat ggacgtaaac gatcgaaata atatcgacgt tagccatttt
     1921 tcccgtgccg ggtacgttat gcccggggaa tatccgctca tattagttgt gaatggaact
     1981 gaaattcgcg aggtcacggt ttcatttgca ccgccggaag atgacccaca aggcagctcc
     2041 ccgtgtttaa caccggaaat ggtaaagctt tttggtctta aagatgaagc aacgaaaaaa
     2101 ctaatctgga aaggtaacgg tgatgatcgt tgtctggatg aaaagtcact tcccggcatg
     2161 accgctaccg gtgaccttag taccggttca cttaagattt ctttacccca ggcctggctg
     2221 gaatatagcg cgccggactg ggatccgcca tcccgcgggg ataacggagt ggcagactta
     2281 ttatttgatt acaacctgaa cgcatcgaaa agccatgcgt tatctgaagg tggttccagt
     2341 gcgaccagct taagtggtag cggaacggca ggcgcaaatt ttgggccatg gcgtttacgt
     2401 gccgactggc aagcctcgcg ttatagcgac aggggataca cgtcgcagag tatggactgg
     2461 agtcgatttt atctctaccg cgcgcttcct aagcttggcg ccaggttaac tctgggtgaa
     2521 tctgatcttg attcatcact gtttgatagc ttccgcttta tcggcggtag tctggtgact
     2581 gatgacaata tgctgccgcc taatcttcag ggatatgctc ctgaagtgac gggggtagcc
     2641 aggtcaaatg cgaaggtcgt gataagccag caagggcgcg tgatatatga atcgcaggtg
     2701 gcagctggtc cttttcgtat ccaggaatta agcagttccg tcagcggtac cctggatgtc
     2761 cgggtggagg agcaagatgg acgggtacaa cattttcagg ttgatacggc cagtattccc
     2821 tatctgacgc gtccggggtt ggtgagatac aaagcgtcct taggaaagcc tacggactgg
     2881 aaacaccatg tacaggatgt gtcttttgcg acgggagagt tctcttgggg tatgacaaac
     2941 ggttggtcac tatttggtgg cctggtcgca gccaaaaaat atgcggctct tgcagtgggt
     3001 attggtcgcg atttgctgtc atttggtgcg gtttcgtttg atgtcaccga atctaaggcg
     3061 acgcttgata ctggcgaaaa aaaaatgggt ggttcatacc gcctgagtta ttccaaacgc
     3121 tttgagaaaa tagatggaca gataacgttc gcgggctatc gcttttcaga aaaagacttt
     3181 atgtcaatga acgattttct cgaaacagag tatgacgccg gtagcaggag tgatactgcc
     3241 tggggaaggc agaaagagct ttacaccatc acagtcagta aagcttttcc tgactggaat
     3301 atgaatctgt atggcaatta tagccaccgg acctactgga atcgtcccga tgcagatcgc
     3361 tatagcctgt ctttatcgaa atacatggac gttgggcgtt ggaaggggat tagcctgaac
     3421 ctgactggct atcgcagtga ggcatggggt gtgaaggatg atggtgtata tgccagtctg
     3481 agcattccct ggagtgatgg cggttcattg agtttcaacg ggcaaagcac aacggctggc
     3541 acaacacaga tggttggttg gtacgacaga attgatagta acaataatta cagtctgcaa
     3601 gccggtacaa gtgttgggaa taaggcctcg gcgagtggat atgtgtcaca caatggtgat
     3661 ctcacgaatg gaacgctgag tgcgagttat tacgaaggaa acaatacgtc gttagggctg
     3721 tctttgcagg gcggaatgac agtaactcgt tacggagcgg cgttgcatcc tggtgcgatg
     3781 aacggcggta cgcgactgat gattgattct ggtgatgttg ctggtgtgcc gttaagccat
     3841 agtggggttc tgcaacgcac gaatgccttt ggcgtcggcg tcattggtaa ccttaatagc
     3901 tactggcgca acagtgttgg ggtcgatgtt aatgccctgg acgatgatat ggaagtcagc
     3961 catccgttaa cggaattgac catgactgaa agtgcgatcg gttatcgaaa aatcgatgtt
     4021 cttgaaggtc gaaaattgat ggcggtagtg aagtttgcgg atggtagtag tccggcattt
     4081 ggcgccaccg tctggagagg gagccatcag accggcgtcg ttgatgataa cggtagcgtg
     4141 tggttagccg gggttaagcc gggtgtcacg atggaagtcc gtgaagatga aaatccggta
     4201 tgtcaggtag gtattcctga gcgattaccg ccggaaaata ccgatcaatc gctgttatta
     4261 acatgccagc agaaatcata actcattatg tgagtggggc actgtattgg aaaaacagtg
     4321 tgtgtagaga ttaggagaaa aggacgcggc catggacggt acattaatgt tgataattaa
     4381 attcagagaa tcaaatgaac agaaataaat caattaagtc ccagccgttc ctgaaatctc
     4441 ttctggtcat ggctttagtc agtcagtccg catttcaggt tgctgatgcg gcagttgctc
     4501 tggatcgtac tcgggtaatt tttgaaggcg gtaataagag catgagctta aatattcgta
     4561 acgataatac gaagctccct tatttagcgc aatcttgggt agaggatgag caaggaaaca
     4621 aaatacagtc accgctgatg gcattgccgc cgctacaacg tatggagccg ggttcaaaaa
     4681 gcctggtgaa aatacagtta gcaccaggcg ttgaagcaaa attgcctcag gatcgtgaat
     4741 cgttgttcta ttttaattta cgcgaaattc cacctaaaag taataaagcg aacgtcctgc
     4801 aactggcgct acagactcgg gtgaaaatgt tttatcgccc gaaagcgctg gcaaaaaacc
     4861 aggcaatgaa tgaaacaccg tggcaggagc agttgacgct t
//
LOCUS       DADLSA010000225         4890 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.226, whole genome shotgun sequence.
ACCESSION   DADLSA010000225 DADLSA010000000
VERSION     DADLSA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.226"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(209..2062)
                     /locus_tag="JN691_003968"
     CDS             complement(209..2062)
                     /locus_tag="JN691_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY7245242.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSEPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADAGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATYAQQPALFTAMLKQFRADLTVFNAQCHGGSAADV
                     PWICGDTTYYWKNTYATQYDTVYGGYKNRESEGVYFVPFMTDGNGVNTATNAPAEDPD
                     IPASGYYGAASRTNGNQVSSNRPTHFSSWARRSIIPDRMATAILNAAGRTSAFISGKA
                     PEIKPSPGGDTPSGPPEDASVRTISLLPTAGDAAAQGWSIKNGGIQLSEGVFKITKQS
                     NKTWSLMHPVDDAITLLTQGGRLTCKFRLSGALTNNQFGLGIYLYTDAPVPDDVAMTG
                     TGNPFLMSYFTQTTDGRVNLMHHRKAGNTKLGEFGDYGNDWQTLELVFTAGSATVTPK
                     LNGLAGPAFQVIKDSLTLGLNALTLTDVTKNAAYGVEIESLVLEINAPASS"
     gene            complement(2551..2627)
                     /locus_tag="JN691_003969"
     tRNA            complement(2551..2627)
                     /locus_tag="JN691_003969"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2591..2593),aa:Arg,seq:tct)
     gene            complement(2641..2717)
                     /locus_tag="JN691_003970"
     tRNA            complement(2641..2717)
                     /locus_tag="JN691_003970"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2681..2683),aa:Arg,seq:tcg)
     gene            complement(2727..2802)
                     /locus_tag="JN691_003971"
     tRNA            complement(2727..2802)
                     /locus_tag="JN691_003971"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2767..2769),aa:Ile,seq:cat)
     gene            complement(2852..3901)
                     /locus_tag="JN691_003972"
     CDS             complement(2852..3901)
                     /locus_tag="JN691_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HAY7245243.1"
                     /translation="MANTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDGRAALGITAKQIADATGKKNMVPHWFSASQWQLPNESDYLKLQSLFARVA
                     EEKHQRGELEKPHHQLVSTYSELNRQYMELLSEYKNLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRAIGVELET
                     GRFEQTVREVQDLIV"
     gene            complement(4010..4250)
                     /locus_tag="JN691_003973"
                     /pseudo
     CDS             complement(4010..4250)
                     /locus_tag="JN691_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4466..4846)
                     /locus_tag="JN691_003974"
     CDS             complement(4466..4846)
                     /locus_tag="JN691_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HAY7245244.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSATKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
BASE COUNT         1121 a         1415 c         1154 g         1200 t
ORIGIN      
        1 gttcccaccg ctgacgtggt gtatgacaca cctgttgtga ttttttccac ctggtacata
       61 ccccgtctcc cgttatccgg aagctgacaa caataaaaaa gccaccagtt aagtactgat
      121 ggctctgata actcatgcag gcgtctcaga cgacccactg acactaccgg tgagtttaac
      181 gataccttcc atttgactgg ctcacttttt atgatgatgc cggtgcattt atctccagca
      241 ccagactttc tatctcaacg ccatacgctg cattttttgt aacatccgtc agcgtcagcg
      301 cattcagccc cagtgtcaga ctgtctttta tgacctggaa tgccgggcca gccagtccat
      361 tcagtttcgg agtaaccgtg gcactgccgg cggtgaacac cagctccagc gtctgccagt
      421 cgttaccgta atcgccgaac tcccccagct tcgtgtttcc ggctttcctg tgatgcatca
      481 gattcactct gccgtcagtg gtctgagtga agtaagacat caggaacgga ttaccagtac
      541 ccgtcatcgc cacatcatca ggaacgggag cgtccgtata cagataaatc cccagcccga
      601 actgattgtt ggtcagtgcg cctgagaggc ggaacttaca ggtcagtctg ccgccctgtg
      661 tcagcagggt aattgcgtca tccaccggat gcatcaggga ccaggtttta ttgctctgct
      721 tggtgatctt aaatacaccc tctgacaact gaattccgcc attcttaatg ctccagccct
      781 gcgcagcagc atctccggct gtcggcaaca gggagattgt gcgtacggat gcatcttcag
      841 gcggccccga tggcgtgtcg ccgccgggcg agggtttgat ttccggtgcc ttaccactga
      901 tgaaggctga ggtgcgcccg gctgcgttca gaatagcggt tgccatacga tccggaataa
      961 tgctcctgcg cgcccatgaa ctgaaatgtg tcgggcggtt tgatgatacc tggtttccat
     1021 tcgttctcga tgccgcaccg taatatcctg atgccggaat atccggatct tctgccggcg
     1081 cgttagtggc ggtattgacg ccgttaccgt ctgtcatgaa gggcacaaaa taaacgccct
     1141 cactctccct gtttttatac ccgccgtaca cggtgtcgta ctgggtagcg tatgtatttt
     1201 tccagtaata cgtcgtgtca ccacaaatcc acggcacatc tgcagcactg ccaccatgac
     1261 actgcgcgtt aaacacagtg aggtcagcac gaaactgctt cagcatggct gtaaacagcg
     1321 caggttgctg tgcgtaggtg gcggcgctca tgtcaaactc tccctgcatc cagcacaccg
     1381 ccagcaacac atttttcggg ttcttctgta atgcagcttt agtgcgcgca atcaggtcct
     1441 gatataacgg tttacccacc ccccagcgcg ccgaatcctg gctggccccc gcgtccgcac
     1501 tgaatgtccc ctccgcgccc tgggtgaatg ccgaaccacc acgacagcat ggtaccagca
     1561 ggatccccgc gttattcggg atatacggga gcagtttttt ggcaatatgt aagccctggc
     1621 cgacacagcc gtactgccct ttgctcaggt ctgccttcgg atgattcagc gtactcatat
     1681 cctgcacatc atgcagacag tggtcagccg ggatgatgtc gttatacgta caactctctc
     1741 cacccggagt taccgtgctg cggcgcgcca gctgtttaat gcgcggatcc ggagcatcgt
     1801 aagaatccgg taacggaagc ccttcaccgt aggccatgcc gttggactgt ccggcaagca
     1861 caaccacgta gaaccagtcc ggctcagatg aagggccgac ctgtggatct ccttcaatag
     1921 ccaccgcctg catcagtgtg tacggcgtaa tggcaaccgg tccgccatat ggctgccagc
     1981 cctctttcag tttgtgtgtc agcttttccg caaggtctga cggcgacgcc gccctgacaa
     2041 catcatagtg tttaaatgcc atgaatcctc ccggccggga taatattgtg agtaaaatga
     2101 ggagcgggct gaagtccgga agttacagga caatggcaga agagagacaa cagcccgcaa
     2161 tacgaaaaag gccgcgctat tgcgcagagt gattactgtc ggatattatt cgccagctga
     2221 aatattactt cacgttttgt tgtttattcc ttgccgcccg cgtctcccag cgcgggcttt
     2281 ttttgtccat aagaaagccc ctccggagag gggctggaga gtggcgctat gtgccattgc
     2341 atggtgccgg gtgcctcccg gtgaattcag taccagcacc tgaatccgcg attatcccat
     2401 atacctactc gctgattgcc cctccgcaca gggggattca ccatgccagt ttcttttaac
     2461 aaactccccg caaaacagac aactgtcaac cgtctgaatt gtgagacatt taaaaaaagg
     2521 cccgcaaaag cgagccggga aaaataagtc tggcgcgttg tactggattc gaaccagtga
     2581 ccgattgctt agaaggcaat tgctctgtcc ggctgagcta acaacgcagg gtacagataa
     2641 tggaccgcca tcgaggactc gaaccccgcg caaccagctt cgaaggctgg cgctctatcc
     2701 cgatgagcta atggcggtat gtgatatggt ggcccttgct ggatttgaac cagcgacctg
     2761 gcgattatga gtcgctcgct ctcaccactg agctaaaggg ccgcgcgcag aataataacg
     2821 ttacggaatt aatactgcaa tctcatccgt ttcaaacgat taaatcctga acttccctga
     2881 ctgtctgctc aaaacgtccg gtctccagct caacaccaat cgcacgacgc cccagtgcca
     2941 tcgccgcttt taccgttgaa cccgacccca tgaaaaaatc cgcaaccaga tcaccaggac
     3001 gactacttgc gctgattatc tgctgcagca tttctgccgg tttttcgcac ggatgtttcc
     3061 ctggatagta ctgcaccggt ttatacgtcc acacatcggt gtacggcacc tgcaccgtca
     3121 caccgaaata ccgccgcaaa tttttatatt cactcagcag ttccatatac tgccggttca
     3181 gctcactgta tgtgctgacc agctggtggt gtggcttttc cagttccccg cgctgatgtt
     3241 tctcttctgc cacccgggca aacagcgact gtaatttcag ataatcgctt tcgttcggta
     3301 gctgccactg actggcactg aaccagtgcg gcaccatgtt tttctttcct gtggcatctg
     3361 caatctgttt tgccgttatc cccagggcag cgcgcccatc acgaaagtaa gcaatcagcg
     3421 gggccatcac atgctgtttc agtgccctgc ccttcgcctc atacccggca tctttcggac
     3481 gatacggccc ctgataatgt tccgcgaaca gaatgcgctc tgtggcgggg aaatacgccc
     3541 gcaggctttc cttgttgcat ccgttccagc gtccggacgg cttcgcccag ataatatggt
     3601 tcagcacact gaagcgttca cgcatcatga tttcgatatc agatgccagg cgatgaccac
     3661 agaacaggta aagacttccg gcaggtttca gcacccgcca gaactgcgcc agacactggt
     3721 ccagccactt caggtaatca tcgtcgccct tccactggtt atcccagccc tcaggcttca
     3781 ctttaaagta cggcgggtcc gtgactatca ggtcaacaga attttcgggt aacgaccgga
     3841 taaattccag gcagtcggcg ttgattaact cacaactgga tatttttaca gtattagcca
     3901 tagatcaata agcacttctc tgataggctc ataccgcttt tgcgcaaagc agatgggcct
     3961 gaggtttgct tgtgacccca acgcatgagc agatggctgg tgggtgcccc taacacccac
     4021 cagccgccca tttaccacaa ataaaaaagc cttcactgcg gaaggcgtct gtaacaaccg
     4081 aactgatagt ctgccagacc cgccataacc agctgggtca gtattaactg gcagcgttcg
     4141 cgtgaaaggt aagtattctg cgcaatctcc ccgactgtcg ccggttcggt gacgcttaat
     4201 tcattaaaca ccactctggc ggtttctgtc atatcctgct gtttcagcat gtctttttcc
     4261 ctttttcggt taacgtgaca caccaataac tcttgtcgaa aaagccagca agctgaaaga
     4321 caggtattca ccgccaccag cacgtttact gtactggacc gatttcagcc ataaaaaaac
     4381 ccgctcgcgg cgggtttaag ctgtgtggcg aagtaaccac tcttaacata ctgacatact
     4441 ttttgcggac cgcactaatc attttttact tttttagcag ccagtcgtcc atctccagtc
     4501 ttacccccag cacagacaaa catccgtcaa taaacccttc ggctatctgc atctcaattc
     4561 gtattgcttt ttcgcttttc tttctcgtcc tggctatctg tctttttgat attcgcaaca
     4621 aataatgagc aatgagaagc gaatactcct caggtttttt ctgcttcaga cgagcaagac
     4681 agttttcaat gataagtccg tcatcatcgc agcaggccgg acgtggttta gtggcagatg
     4741 gtaaaagtcc tttgaatccg gcagcgatcg gagaatagtc caccccggtg ttaccacttg
     4801 cagcccatgc cccccagcgt tcaagaacca tctgaatatc acgcatcaac tttctccaca
     4861 aaatcaggac agcacaccaa tcgccagcgc
//
LOCUS       DADLSA010000226         4860 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.227, whole genome shotgun sequence.
ACCESSION   DADLSA010000226 DADLSA010000000
VERSION     DADLSA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.227"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(404..1495)
                     /locus_tag="JN691_003975"
                     /pseudo
     CDS             complement(404..1495)
                     /locus_tag="JN691_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1527..2072
                     /locus_tag="JN691_003976"
     CDS             1527..2072
                     /locus_tag="JN691_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY7245245.1"
                     /translation="MKDVKVMPESIPAGYEVLQELDELDSLLIIDLGGTTLDISQVMG
                     KLSGISKIYGDSSLGVSLVTSAVKDTLSLARTKGSSYLADDIIIHKKDNNYLKQRIND
                     ENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHVMVIGGGAELICDTVKKNTQIRNE
                     RFFKTNNSQYDLVNGMYLIGN"
     gene            2075..2426
                     /locus_tag="JN691_003977"
                     /pseudo
     CDS             2075..2426
                     /locus_tag="JN691_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein stbB"
     gene            complement(2576..2749)
                     /locus_tag="JN691_003978"
     CDS             complement(2576..2749)
                     /locus_tag="JN691_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245246.1"
                     /translation="MLSGQIFCIPLNNLVGDKINYDEITKITARDWRQYRAPGWQITH
                     QKRYCQTLRTHQF"
     gene            complement(2802..3077)
                     /locus_tag="JN691_003979"
     CDS             complement(2802..3077)
                     /locus_tag="JN691_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY7245247.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
     gene            complement(3077..3361)
                     /locus_tag="JN691_003980"
     CDS             complement(3077..3361)
                     /locus_tag="JN691_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HAY7245248.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDHPLPVPR"
     gene            3813..4064
                     /locus_tag="JN691_003981"
     CDS             3813..4064
                     /locus_tag="JN691_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7245249.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGSFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
     gene            4033..4323
                     /locus_tag="JN691_003982"
     CDS             4033..4323
                     /locus_tag="JN691_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAY7245250.1"
                     /translation="MNAHWSSKKSNFFRKNIKLLTKYLFFESQGIPDKVDIVSRLKTY
                     GYSISGVETDDGYKALVRAFQLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
     gene            complement(4423..4608)
                     /locus_tag="JN691_003983"
                     /pseudo
     CDS             complement(4423..4608)
                     /locus_tag="JN691_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         1257 a         1174 c         1167 g         1262 t
ORIGIN      
        1 ttatacgata attagcatac ttataacacc gtaagtaaat tacatgaaag caggcacagc
       61 gtgatccgat ttttctgcgt ataacgattt tccggaagtg taatacggtt gcgccccggg
      121 ccgggcaccc ttttctgttg ttgtgtacgg gcccacagag aatggtaccg gaggcgggct
      181 cagaagtact gagtcgggtg atcgcgtcag gccgcgaagt actgacacgg gtattggtga
      241 ctggtgatga agtactgccc cgggtatgat ttcaggctga ttcaggttct gattctctgt
      301 cagtccgcgt ggttgctggc ctagctgatt tcctgttgtc aggcgttcaa caaccgactg
      361 caggttatag cccggttcac gaacacctga cggcgggtat cactcacccg gtgaaggtaa
      421 atccctcaag aggcagcaac tctgccagtc gctcctccgg ccattccggc agacgcgtca
      481 ggacgtccgt cagccaggca tgtggctcca gaccattacg ttttgctgtt tccagaaggc
      541 tcattatctg tgccgcccgg tttccggcca tcagtgaacc ggcgaacagc cacgctttcc
      601 gtcccataac aaccgggcga atggcacgct cgcagatatt gttgtccagg ggcaccgctc
      661 cgtcacccag gaagcagctc agttcatcgc gccggttcag aatatagtta atcgccttat
      721 ttaatggccc gtcgggcgga cagcacggct cctgctcttc aagccaggca aacaggtcat
      781 tcactatcgg ccgggagtac cgttatcgcc actgccgtat tttttccaca gggcgctcac
      841 ggatgagttt ctcaatgcgg taaagacccg cgatttttct cagctccacg cctgcacggg
      901 gctccctgct ggccttatac agatttgcga agccgcgccc tgcgtgactc cagcaacagg
      961 ctgatgtgat gccgggatga ttatccgcca gagatttgta cacgctgtat ccgtcactca
     1021 ccagcgtacc gccacaccag cactgcagcc aggtctcagg gtatctgagg gcccgtccgg
     1081 tctgcgagtc aaaacagacg accggcggac cgcttctttc accgctgacg taagcacaca
     1141 gccagccgga gcaggattta ccgcctttcc gggtatcaag gagcctcagg gacgtttcat
     1201 ctgcatgaat aacatcccgt gtcagaagct catggcgtag cagtttcgcc agcggggcca
     1261 gtgcagctcc ggcaacaccg accatatccg ccattgtact gacggggagc tccacaccca
     1321 ttcgggagaa aatgtgctgc tggcgataca ggggcagatg gtcggtatat ttgctgatga
     1381 caacctgtgc aattaccgac gactcaacag cactttcagg gaggatatgc gccggcattt
     1441 tcccgctgaa cactttctca cagcagggac agctgtactg cggacggata tagcggattg
     1501 cattaaatgg tggggataca ttcacaataa aagatgtaaa agtcatgcct gaatctatac
     1561 cggcaggtta tgaagttcta caagaactgg atgagttaga ttctttatta attatagatc
     1621 tcgggggcac cacattagat atttctcagg taatggggaa attatcaggg atcagtaaaa
     1681 tatacggaga ctcatctctt ggtgtatctc tggtcacatc tgcagtaaaa gatacccttt
     1741 ctcttgcgag aacaaaagga agtagctatc ttgctgacga tataattatt cacaaaaaag
     1801 ataacaacta tctgaagcaa cgaattaatg atgagaacaa aatatcaata gtcaccgaag
     1861 caatgaatga agcacttcgt aaacttgagc aacgtgtatt aaatacgctc aatgaatttt
     1921 ctggttatac gcatgttatg gttataggcg gtggcgcaga attaatatgc gatacagtaa
     1981 aaaaaaacac acagattcgt aatgaacgtt ttttcaaaac caataactct caatatgatt
     2041 tagttaacgg tatgtatctc ataggtaatt aataatggac aagcgcagaa ccattgcctt
     2101 caaactaaat ccagatgtaa atcaaacaga taaaattgtt tgtgatacac tggacagtat
     2161 cccgcaaggg gaacgcagcc gccttaaccg ggccgcactg acggcagatc tggccttata
     2221 cagacaagat ccccggcccc ccatttcttt tatgtgaact gctgacgaaa gaaaccacat
     2281 tttcggatat cgtgaatata ttgagatcgc tatttccaaa agagatggtc gattttaatt
     2341 cttcaacaat cactcaatcc tcttcacaaa aagagcaaaa aagtgatgaa gagccccaaa
     2401 agaacgtgat gaagctaata aactaattca attattattg agtttccttt atccactatc
     2461 aggctggata aaggaaactt aatcaagtta ttttcttgcc aatcattaca taaaataaac
     2521 agggtcgtta ttatgaaatt aatccacggt gcttatgaaa aagcggctcc aataatcaaa
     2581 attggtgagt tcgcaatgtc tggcaataac gtttctgatg ggttatttgc cagcccggag
     2641 cgcgatattg ccgccagtca cgagcggtaa ttttcgttat ttcatcgtaa tttattttat
     2701 ctcctacaag attatttaat ggaatgcaaa atatctggcc tgataacaat gctccagaat
     2761 taatacctgc aggctatgtt aaagttgcct ccacacccaa tctacctgtt ctctcgtgtg
     2821 tgccacaggc gcaatacata aatagtctgg ccagtaagtt cgtaacggat ttcgtactcg
     2881 ccagtaaaaa tccgtctgac ctccctgggt tcaaactgaa acaactgttc tcccatacgt
     2941 ggatgagtta acagaatgac cggcgcctgt gtcagggact gcaccgttct ggcggcagca
     3001 ggtttactgg ccagcaccag aaaatcatat aaccgcgcca aatcagaaag tgccttactg
     3061 gtccacttca gttccatcag cgtggcaccg gtaacggatg gtcagtactg aggctgtccg
     3121 cccaggcctg tacagcctga tggtcgataa cctgtccgga tgtcacatcg tccagggctt
     3181 ccagcgtcag gcgattacgc tcctcctctt gggcaagcca tgcagaaagc gcctgtttga
     3241 taacccagcc ccgggagcgt tccagtctgg cggccatctg gtcgacttta tcagccatag
     3301 gtaatggcac atgcgcggta attacttttg ttgcttgtgc ggtattgttt ttcatttgca
     3361 tacccgatta gttgttaatc atatttaatc atagtgatta ctttcagacg gaacaaggca
     3421 aataatgcga gtcagcgcat ataaggcggc atacggtggt tacatgtagc acaggtgtaa
     3481 aaccgccgaa gcccggcgca agccggtacg gtcgtccgaa tatcaccagt ccccctccgg
     3541 ctctgcgagg ccatgcggcc tgtcagcgat gaaaatgcct ctgtgcgcgg tcggagtggt
     3601 cccgacaagg gtttacccgc agtcgaggcg tatctccgcg ttagcgggcc gtgagggccg
     3661 cttacgagcg tgtatgagag acgtacagta gttttgtccg ctcacaccag tacaaaccag
     3721 tcgaaatacc ggtaacgacg attactcttg tctctgatgc cggtggacta cgagatttta
     3781 tttactggcg tcctgatgcg gtcaggactg gtatggaacc tgtttatgtg atacttaatg
     3841 ctcttctgga ttccgggcga tttacgcgta aacttattct gttagggctg tcaggctcat
     3901 tttcctatat cttcggctca attgttgcga cactgggaat gggacttgtt gtcgattatc
     3961 tgggatgggg ggccacattt atcgtactga tactgtctgc tgtttttgcc atcattttca
     4021 cactgatgag tcgtgaacgc tcactggagt tcgaaaaaga gtaatttttt caggaaaaac
     4081 ataaaattac tgactaaata tttatttttt gaatcgcagg gcattccgga taaggttgat
     4141 atcgtgtcac ggctgaagac ctatggatac agtatttctg gtgtggagac ggatgacgga
     4201 tataaggcgc ttgtccgcgc ttttcagctt catttccggc agaaaaatta tgacggcata
     4261 atggatgctg aaaccgctgc catcctgtat gcattgctgg agaagtattt cccgggtaaa
     4321 taaggagtga gtcgtcagag ctgcgatata tccccttcct ccatgggctg tctcttatac
     4381 acaaatcccc acccggggat ttgtgatgtc agatgtcccc tgtaaaacta tgttttcatt
     4441 gcgctctatg aagctgagtg agcgtaacga ctggagctgc ttctgcggtg ctccggtggc
     4501 gaacgtgaag tcatactctt cgaacatttt caccgccggg aaggctacca ttcgggtata
     4561 catcgcctgt ttacgttgat gacgtgccag tttttcttca tgaagcatga tgcttcaaga
     4621 agggcactgt tgcaaagtta tcgatgaggt agctgaccat cactgctgtc ggaatcgatc
     4681 ttgcccttct gtttccagtc gcctcagcaa actatttttc agttaccacc tgtcctgcgg
     4741 taagcgtaaa ccgaccaccg tatgtagcca ttagacaaga attggtaact tagacgccca
     4801 tctgacatag acgaacacct aagtatggaa ttacaggact ggcgaaaaga acctcgtaaa
//
LOCUS       DADLSA010000227         4783 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.228, whole genome shotgun sequence.
ACCESSION   DADLSA010000227 DADLSA010000000
VERSION     DADLSA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.228"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            29..286
                     /locus_tag="JN691_003984"
     CDS             29..286
                     /locus_tag="JN691_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7245251.1"
                     /translation="MTLPVFITVIADHDKPQPSGCLLESQGSLCPICRQRITHETGWN
                     VHHKVKKVMGGGEELSNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            428..649
                     /locus_tag="JN691_003985"
                     /pseudo
     CDS             428..649
                     /locus_tag="JN691_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(670..732)
                     /locus_tag="JN691_003986"
                     /pseudo
     CDS             complement(670..732)
                     /locus_tag="JN691_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1183..2595
                     /gene="ospC1"
                     /locus_tag="JN691_003987"
     CDS             1183..2595
                     /gene="ospC1"
                     /locus_tag="JN691_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HAY7245252.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2699..2848)
                     /locus_tag="JN691_003988"
                     /pseudo
     CDS             complement(2699..2848)
                     /locus_tag="JN691_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2924..4621
                     /gene="ospD3"
                     /locus_tag="JN691_003989"
     CDS             2924..4621
                     /gene="ospD3"
                     /locus_tag="JN691_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HAY7245253.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFAENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1664 a          869 c          888 g         1362 t
ORIGIN      
        1 aacgggccat ataaccacct gaaagacaat gacattgcct gtttttataa cggtaattgc
       61 agaccatgac aaaccgcagc cgtcaggctg cctactcgag tcacaaggct ctctgtgtcc
      121 gatatgccgg cagcgcatta cccatgaaac tggctggaac gtacaccata aggtgaagaa
      181 agttatgggg ggcggtgaag aattatctaa ccttgtcttg ttacatccaa attgccatcg
      241 acagttacac agttgtgaag ccggctctct caaaaagggc ttataaaggc ttgagggtgt
      301 accgggaaac cggtatgcac cgttcttagg ggagggcagt ccagcaatga actgtcctta
      361 cccgacaaac atccaaaacg aacggaacgt gctgaaatcc gcatataacc cgttgtaaag
      421 tgaggaaatg gacacctcac ttgctcatga gaacgcccgc ctgcgggcac tgttgcagac
      481 gcaacaggac actatccacc agatggctga atacaaccgc ctgctctcac agcggatggc
      541 ggcttatgct tccgaaatca accgactgaa ggcgctggtt gcgaaactgc agcgtatgca
      601 gttcgataaa agctcagaaa aacttcgcgc aaaaaccgaa cggcagattc cattttcacg
      661 ggcgatctac gccacacagg ctctgggttg cagtgactgc tccattaagg cgattttgaa
      721 ctcataaggg aagtaacaaa aaaactgcaa aagtacaata attatatatg gccaaactaa
      781 cctctattaa aaaaacatca gtttttcaca cttagttgag cttcatgaaa aaatcatcga
      841 gggtttcatt gcagaataca cattccacta actggaagca ctccataaga ggaatcagag
      901 aaagtatcgt ttttttatag taaaattcat tgctgttcaa taaaattgat atagttatta
      961 aattatgaaa ataatttcac aaaaaagatt aacttgagtt cttaagcatc ggattttaga
     1021 tattttgaaa tatctggcat cacataacat caaaagtata taataccctt atctaattac
     1081 atgcagtata ttgtatatat actcaaaaat tgtaattcat accctgctat atcttgtagg
     1141 gaaatatatt aattaaaact gttttcatat aaggttcatt ttatgaatat atcagaaaca
     1201 ctgaactcag caaataccca atgcaatata gattctatgg ataacagatt acatacattg
     1261 tttccaaaag tgacatcagt gcgaaacgct gcacaacaaa ctatgccaga tgaaaaaaat
     1321 ttaaaagata gtgcaaatat tattaaagat ttctttagga aaactatagc agcacagagt
     1381 tatagtagaa tgttctctca aggctctaac tttaaatctt taaatatagc aattgatgca
     1441 ccatcagacg ctaaagcctc atttaaggct attgagcacc ttgacagatt atcgaagcat
     1501 tatatatctg aaataaggga aaaacttcat cctctttctg cagaggaact caatttgctt
     1561 tcgctaatta ttaattctga tttaatcttc agacatcaaa gtaattctga tttgtctgat
     1621 aaaattttaa acattaagtc attcaataaa attcagtctg aaggaatatg cacaaaacga
     1681 aacacatacg ctgatgatat aaaaaaaata gctaatcatg actttgtgtt ttttggcgtt
     1741 gaaatctcta gccatcagaa aaaacacccc ctgaatacaa aacatcacac tgttgatttt
     1801 ggtgcaaatg cgtatatcat tgatcatgac tctccatatg gatatatgac attaaccgat
     1861 cactttgata atgctattcc acctgttttt taccatgagc accaatcatt tttagataaa
     1921 ttttcagagg ttaataaaga agttagtcga tacgtacatg gaagtaaagg aattatagat
     1981 gtaccaatat tcaatactaa agatatgaag ttagggctcg gattatacct gattgacttt
     2041 attagaaaaa gtgaagacca aagcttcaag gagttttgct atggaaaaaa tcttgcccct
     2101 gtggatctgg atagaatcat aaactttgtt tttcagccag agtaccatat acctaggatg
     2161 gtaagtacag aaaacttcaa aaaagttaag attagagaaa tatccttaga ggaggctgtt
     2221 acagcatcta attacgaaga aattaacaag caggtcacta acaaaaaaat tgctctccag
     2281 gctctttttc tttcgattac taatcaaaaa gaggatgtcg ccttatatat attatctaat
     2341 tttgagataa ctagacaaga tgttatttcc ataaagcatg agttgtatga tattgagtat
     2401 ctacttagcg ctcataattc aagctgtaaa gtacttgagt attttatcaa taagggattg
     2461 gttgatgtaa acacaaagtt caaaaaaact aatagtgggg attgtatgtt ggataacgca
     2521 ataaaatatg agaatgcaga aatgataaaa ctattattga aatatggtgc aacatctgac
     2581 aataaatata tttaatcaaa attgaatatc gtttagcaaa aggcagtatt tgcacaaaat
     2641 atcgatatat tgtttattgt cagtatggct caatgtgata atagttggaa agtttgatgg
     2701 gtttcgcccc gttgtagcgg tagtcgagct ggaaggtctt caggcactgc ttacagcgat
     2761 agagcagccc ccctgaactg gaatggccgt tccggatacc cccctgagtt tcagagtaac
     2821 ggggacaaac cacatcaatc tttgccatca atcatccaaa gggcaaagag tacaacaaca
     2881 ctaagtctgc gtcacaaccc atcaatgaaa ggaatatata catatgccat cagtaaattt
     2941 aatcccatca aggaaaatat gtttgcaaaa tatgataaat aaagacaacg tctctgttga
     3001 gacaatccag tctctattgc actcaaaaca attgccatat ttttctgaca agaggagttt
     3061 tttattaaat ctaaattgcc aagttaccga tcactctgga agacttattg tctgtcgaca
     3121 tttagcttcc tactggatag cacagtttaa caaaagtagt ggtcacgtgg attatcatca
     3181 ctttgctttt ccagatgaaa ttaaaaatta tgtttcagtg agtgaagaag aaaaggctat
     3241 taatgtgcct gctattattt attttgctga aaacggttca tggggagata ttatttttta
     3301 tattttcaat gaaatgattt ttcattccga aaaaagcaga gcactagaaa taagtacatc
     3361 aaatcacaat atggcattag gcttgaagat taaagaaact aaaaatgggg gggattttgt
     3421 cattcagctt tatgatccca accatacagc aactcattta cgagcagagt ttaacaaatt
     3481 taacttagct aaaataaaaa aactgactgt agataatttt cttgatgaaa aacatcagaa
     3541 atgttatggt cttatatccg acggtatgtc tatatttgtg gacagacata ctccaacaag
     3601 catgtcctcc ataatcagat ggcctaataa tttacttcac cccaaagtta tttatcacgc
     3661 gatgcgtatg ggattgactg agctaatcca aaaagtaaca agagtcgtac aactatctga
     3721 cctttcagac aatacgttag aattactttt ggcagccaaa aatgacgatg gtttttcagg
     3781 attgctttta gctttacaaa atgggcattc agatacaatc ttagcatacg gagaactcct
     3841 ggaaacttct ggacttaacc ttgataaaac ggtagaacta ctaactgcgg aaggaatggg
     3901 aggacgaata tcgggtttat cccaagcact tcaaaatggg catgcagaaa ctatcaaaac
     3961 atacggaagg cttctcaaga agagagcaat aaatatcgaa tacaataagc tgaaaaattt
     4021 gctgaccgct tattattatg atgaagtaca cagacagata cccggactaa tgtttgctct
     4081 tcaaaatgga catgcagatg ctatacgcgc atacggtgag ctcattctta gcccccctct
     4141 cctcaactca gaggatattg taaatttgct ggcctcaagg agatatgaca atgttcccgg
     4201 acttctgtta gcattgaata atggacaggc tgatgcaatc ttagcttatg gtgatatctt
     4261 gaatgaggca aaacttaact tggataaaaa agcagagctg ttagaagcga aagattctaa
     4321 tggtttatct ggattgtttg tagccttgca taatggatgt gtagaaacaa ttattgctta
     4381 tgggaaaata cttcacactg cagaccttac tccacatcag gcatcaaaat tactggcagc
     4441 agaaggccca aatggggtat ctggattaat tatagctttt caaaatagga attttgaggc
     4501 aataaaaact tatatgggaa taataaaaaa tgaaaatatt acacctgaag aaatagcaga
     4561 acacttggac aaaaaaaatg gaagtgattt tctagaaatt atgaagaata taaaaagctg
     4621 aatattatct cgtggtgata cactgaattt gaggtaaccg gctcatttaa accgtctggt
     4681 ctgttacctc cggttccaca aaaataatgt ccatcatttt gaagtggaca ctatcgtatg
     4741 gaataccgga cctggatcac tgaagcttta cgccttcatt tcg
//
LOCUS       DADLSA010000228         4776 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.229, whole genome shotgun sequence.
ACCESSION   DADLSA010000228 DADLSA010000000
VERSION     DADLSA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.229"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            216..3110
                     /locus_tag="JN691_003990"
     CDS             216..3110
                     /locus_tag="JN691_003990"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7245254.1"
                     /translation="MVVLLTPGDDPLRAFLIWVLSESIGALALVPLGLLFKPHYLLRH
                     RNPRLLFESLLTLAITLTLSWLSMLYLPWPFTFIIVLLMWSAVRLPRMEAFLIFLTTV
                     MMVSLMMAADPSLLATPRTYLMSHMPWLPFLLILLPANIMTMVMYAFRAERKHISESE
                     TRFRNAMEYSAIGMALVGTEGQWLQSNKALCQFLGYSQEELRGLTFQQLTWPEDLNKD
                     LQQVEKLISGEINTYSMEKRYYNRNGDVVWALLAVSLVRHTDGTPLYFIAQIEDINEL
                     KRTEQVNQQLMERITLANEAGGIGIWEWELKPNIFSWDKRMFELYEIPPHIKPNWQVW
                     YECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIAVKDGIRHIRALANRVLNKEGEVERL
                     LGINMDMTEVKQLNEALFQEKERLHITLDSIGEAVVCIDMAMKITFMNPVAEKMSGWT
                     QEEALGVPLLTVLHITFGDNGPLMENIYSADTSRSAIEQDVVLHCRNGGSYDVHYSIT
                     PLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSASHDALTHLANRASFEKQLRILLQTV
                     NSTHQRHALVFIDLDRFKAVNDSAGHAAGDALLRELASLMLSMLRSSDVLARLGGDEF
                     GLLLPDCNIESARFIATRIISAVNDYHFIWEGRVHRVGASAGITLIDDNNHQAAEVMS
                     QADIACYASKNGGRGRVTVYEPQQAAAHSERAAMSLDEQWRMIKENQLMMIAHGVASP
                     RIPEARNLWLISLKLWSCEGEIIDEQKFRRSFSDPALSHALDRRVFHEFFQQAAKAVA
                     SKGISIALPLSVAGLSSATLVNDLLEQLENSPLPPRLLHLIIPAEAILDHAESVQKLR
                     LAGCRIVLSQVGRDLQIFNSLKANMADYLLLDGELCANVQGNLMDEMLITIIQGHAQR
                     LGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIADAQPLDLLVNSSYFAIN"
     gene            complement(3220..4068)
                     /gene="alkA"
                     /locus_tag="JN691_003991"
     CDS             complement(3220..4068)
                     /gene="alkA"
                     /locus_tag="JN691_003991"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HAY7245255.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            4202..>4776
                     /gene="yegD"
                     /locus_tag="JN691_003992"
     CDS             4202..>4776
                     /gene="yegD"
                     /locus_tag="JN691_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7245256.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQLPEAITQAVIGRPINFQG
                     LGGDEANAQAQGILERAAKRAGFKDVVFQYEP"
BASE COUNT         1115 a         1252 c         1291 g         1118 t
ORIGIN      
        1 atgggttaac cagataacca cggggagcag gtaccggcat ccacgttaca gacggtcccg
       61 gcaaaagtgc ctgacctgac ccctattagc ggttaccagc gccccaccag acccggtgac
      121 atcacccccc ggatcatgga cgactggggg cagtaatcat gcctggtctg gctggctaac
      181 accccattat aaggggtacg aataaagtaa cggggctggt tgtcctgctg acgcccggag
      241 acgatcctct cagggcattt ttgatatggg tactgtcaga atccatcggc gctctggcac
      301 tggtgccgct gggattgtta tttaaaccac actatctgct gcgccatcgc aacccacggt
      361 tgctttttga gtcgctgctc acgttagcca tcacactgac gttaagctgg ctttcgatgc
      421 tgtatctgcc gtggcctttt actttcatta ttgtgctgtt gatgtggagc gccgtgcgcc
      481 tgccacgaat ggaagccttt ttgatcttcc ttaccacggt gatgatggtg tcgctgatga
      541 tggccgcgga tccctccctg cttgctacgc cgcgtacgta cctgatgagc catatgccgt
      601 ggctaccgtt tttgctgatc ctgctgcccg ccaacatcat gaccatggtg atgtatgcct
      661 ttcgtgcgga acgcaaacac atttccgaaa gcgaaacccg ctttcggaac gcgatggaat
      721 attccgctat cggtatggcg ttagtgggca ccgagggaca atggctgcaa tccaacaaag
      781 cactctgcca gtttctcggt tacagtcagg aagagctgcg cggactcacc tttcagcaac
      841 tgacctggcc ggaggatctc aataaagatc tccaacaggt tgaaaagctg ataagcggtg
      901 aaataaacac ctattcaatg gaaaaacgct actacaaccg caatggcgat gttgtctggg
      961 cgttgcttgc cgtctcactg gtgcgccaca cggatggcac gccgctctat tttatcgctc
     1021 agattgaaga cattaacgag ctaaaacgca ccgaacaggt gaatcagcaa ctgatggagc
     1081 gcatcacgct ggctaacgaa gcgggcggga ttggcatctg ggagtgggag ctgaagccga
     1141 atatttttag ctgggataag cggatgttcg agctgtatga aattcctccg catatcaaac
     1201 cgaactggca ggtgtggtac gagtgcgtgc tgccggaaga tcgccagcat gccgaaaaag
     1261 tgattcgtga ttcgttgcaa tcacgctcgc cctttaagct ggaatttcgc attgccgtga
     1321 aagatggcat ccgccatatc cgcgccctcg ccaaccgggt actgaataaa gaaggcgaag
     1381 tcgaacgcct gctcggcatt aatatggata tgaccgaggt taaacagctt aacgaggcat
     1441 tgtttcagga aaaagagcgc ctgcacatta cgctggattc catcggtgaa gccgtggtct
     1501 gtattgatat ggcgatgaaa attaccttta tgaatccagt ggcggagaag atgagcggct
     1561 ggacgcagga agaagcatta ggtgttccgc tcctgacggt gttgcatatt acttttggcg
     1621 acaacggacc attaatggag aacatttaca gtgccgacac ctcacgttcc gcgattgaac
     1681 aagatgtggt gttgcactgc cggaacggcg gcagttacga cgtgcattac agtattacgc
     1741 cgttaagtac tctggacggc agcaatatag gctcggtact ggtgattcag gacgtcaccg
     1801 aatcacgcaa aatgctgcgc cagctgagct acagcgcctc ccatgatgca ctgacgcatc
     1861 tcgccaatcg cgccagtttt gagaagcaac tgcgtatcct gctgcaaacg gtaaacagta
     1921 cacatcagcg acatgccctg gtgtttatcg atcttgatcg ctttaaagcg gtgaatgaca
     1981 gcgccgggca tgcggcgggt gacgctttac tgcgcgaact ggcgtcgttg atgctgagta
     2041 tgctgcgctc cagcgacgtg ctggcgcgac tcggtggtga tgaatttggt ctactgttgc
     2101 cagactgtaa tatcgaaagt gcgcgtttta tcgctacacg cattatcagt gccgtgaatg
     2161 attatcactt tatatgggaa ggccgtgtgc atcgggtagg tgccagtgcc gggattacct
     2221 tgattgatga caacaatcat caggcggcag aagtgatgtc gcaggctgat atcgcctgtt
     2281 atgcctccaa aaatggtgga cggggccggg tgacggttta cgaaccacag caagctgccg
     2341 cacatagcga acgggcggcg atgtcgcttg atgaacagtg gcggatgatt aaagagaatc
     2401 agttgatgat gatcgcccac ggtgtcgctt cgccgcggat cccggaagcg cgtaatttgt
     2461 ggctgatttc acttaagctc tggagttgcg aaggcgagat tattgatgaa caaaaatttc
     2521 gtcgtagctt cagcgatccg gcacttagcc atgctcttga ccgacgggta ttccacgaat
     2581 ttttccagca ggccgcaaaa gcggttgcca gtaaaggcat aagcatcgcc ctcccccttt
     2641 ccgttgccgg tttgagtagc gccacgctgg tgaatgatct gcttgagcag ctggaaaata
     2701 gccctctacc accacggtta ttacatctga ttattccggc tgaagcgatt ttagatcacg
     2761 cagaaagcgt gcaaaaactg cggctggcgg gatgtcggat agtgctcagc caggtgggcc
     2821 gcgatctgca aatcttcaac tcgctgaaag cgaatatggc agattacctg ctacttgatg
     2881 gtgagttatg cgccaacgtg cagggtaatt tgatggatga gatgctgatt acgattattc
     2941 aggggcacgc tcagcgactc gggatgaaaa ccatcgccgg gccagtcgtt ttacccttag
     3001 tgatggatac gctttctggc atcggcgtcg atctgattta tggtgatgtg attgccgatg
     3061 cccaaccgct ggatttgctg gtgaatagca gttatttcgc gattaactga gagagcgttt
     3121 gcccattgcc tgatgcgatg ctaacgcgtc ttatcaggcc tacaaatcgc tcattccgca
     3181 ggcgggataa ggcgctcgca ccgcttccgg cgaccaacgt catgcttcgt ctggttgcca
     3241 gccttccgta taccagatat gcaacagcgc ataagaacgc cagggcttcc agcgctcggc
     3301 ataacggcgg atttgtgccg gtgtcatccc cagaaatcgc tgtttaatca gataatcatc
     3361 cggcagaaaa acatctttcg cctgccagcc gcgcaaggcg aaataattcg ccgtccagcg
     3421 cccgataccc ggaaaagttt gcagcgtttt catcgcctgc tccacatcgc ccggtattgt
     3481 cattggtaag gtgccctcca gcgccgcatt tgccagatga atcagcgcct ctgcccgttt
     3541 caacggcata cctaacgctt ttaatgcctg cgggtcggct gctgccagcc gctgaggcgt
     3601 ggggaagcag atatactccg gaaaatcatc cagccgttcg ccataaagct gtgccactct
     3661 ggcggtcaat tttgccgcca tcgccacgct caccagttgg cctaaaatcg cccgcacgcc
     3721 ctgctcaaaa gcatcaacac tgccgggtaa acgcaatccg ggccgcgccg cgcctaactt
     3781 gcccaacgca ccgttaacaa tctgcgggtt acattgcaga tcaagcaggc ggctcatttt
     3841 cgccagacac tcagcggcaa cgggttctaa acctgcactt aaatttatgt gcagagtatg
     3901 gcgggctata tccggaatag cagtcaccac gccgcgatat tcgcccactg ccagactacg
     3961 ggcataataa ctgtccgcga ccgtttccac accactcacc gcacgggcgg cgagaaatcc
     4021 caacatccac gaccagtcat acggcggctg ccagttcagg atatacatcg catctcctta
     4081 ttcatccgct ttcagcataa acgttattca gacgttacgc tttgctttca tattccggtt
     4141 gtcgcgacgg caacatttcg ctaaagtcac gccccttctt caccggcatg gggattattt
     4201 cgtgtttatt ggttttgatt acggtacagc aaactgttca gtggcggtca tgcgtgacgg
     4261 taaaccgcat ttgctaaaaa tggaaaacga cagcacgctg ctgccttcaa tgctttgcgc
     4321 gccaacgcgt gaagcggtaa gcgaatggct gtaccgccat catgacgttc cggcagacga
     4381 cgatgaaacg caggcgttgt tacgacgggc gattcgttat aaccgcgaag aagatatcga
     4441 tgttacagcg aaaagcgtgc agtttggtct ttcctcactg gcacagtaca ttgatgatcc
     4501 agaagaagtg tggtttgtga aatcaccaaa atcgttcctc ggtgccagcg gcttaaaacc
     4561 gcagcaggta gcgctgtttg aggatctggt ctgcgcaatg atgttgcaca ttcgccagca
     4621 ggcgcagctg ccagaagcga ttactcaggc ggtgattggt cgcccgatca acttccaggg
     4681 gctgggcggc gatgaagcaa acgcccaggc gcaggggatt ctggaacgcg cggcgaagcg
     4741 tgccgggttt aaggatgtgg tattccagta cgagcc
//
LOCUS       DADLSA010000229         4704 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.230, whole genome shotgun sequence.
ACCESSION   DADLSA010000229 DADLSA010000000
VERSION     DADLSA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.230"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(1..1131)
                     /gene="traD"
                     /locus_tag="JN691_003993"
                     /pseudo
     CDS             complement(1..1131)
                     /gene="traD"
                     /locus_tag="JN691_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
     gene            complement(1559..1750)
                     /locus_tag="JN691_003994"
     CDS             complement(1559..1750)
                     /locus_tag="JN691_003994"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245257.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRHR"
     gene            complement(1747..2169)
                     /locus_tag="JN691_003995"
     CDS             complement(1747..2169)
                     /locus_tag="JN691_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAY7245258.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSEFVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMEVVLQALDGEHMS"
     gene            complement(2216..2641)
                     /locus_tag="JN691_003996"
     CDS             complement(2216..2641)
                     /locus_tag="JN691_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAY7245259.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIVFHPECNAIYAALD"
     gene            complement(2614..3048)
                     /locus_tag="JN691_003997"
                     /pseudo
     CDS             complement(2614..3048)
                     /locus_tag="JN691_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052648.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1472 domain-containing protein"
     gene            complement(3219..4001)
                     /locus_tag="JN691_003998"
                     /pseudo
     CDS             complement(3219..4001)
                     /locus_tag="JN691_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            4000..4647
                     /locus_tag="JN691_003999"
                     /pseudo
     CDS             4000..4647
                     /locus_tag="JN691_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001440616.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1090 a         1327 c         1274 g         1013 t
ORIGIN      
        1 agcctgagtc acgtcttctc cggaaacaac ctccgggaca tccggttcga agaggctggc
       61 catctgacga ccttctgctt cccttgcggc aagtacggca ctcagacggt tctccatttc
      121 cgggttgatg tcacgcggaa taaactccgg agcgaccttc ggacgttcct gatatttcag
      181 tgagagtttg actgccggat acggtccggg cagggtgaca tagcaggtca gatccggcag
      241 ggactgaatg tcggaataac tgaccagcgt ctggcgctcc atatccttac cggtcgatac
      301 cccgtcacgt accggatcag caccgtagga atactgttcg ctggctttca ggtgctcttt
      361 ctcaccaatt tcaccggcag cgaactccgc aatcttatgg ctgggagaac ggaaaaaggc
      421 acgggtgttc atgacgtcaa acagcgtggc agccgctttc tcaccgtaga tatcttccag
      481 ctgggcatag gactggatac caaacacata acagccaccg aacttacggg cttccggcag
      541 gatctccacc aggtccggca gtttgtgtaa tgtgggtaac tcatcacaga agaaccacac
      601 acgacgatta cggttttctc ccattgccag cagaccacga atggcaatgg acagccacat
      661 ggagatcacc ggcttcaggg aggcatgagt gtcggcattc gacgagataa acagccagcc
      721 gtttttctga tcctcccgga caccacgcat ccagtcacgg atggtgaagg gctcaccgtt
      781 atgctcaatc ccctgcaggt aacggatggc tttcacatag ttggtcagca cagcacggat
      841 ggagatggcc gttttctcga ttttctcttc caccaggttg gccgccggtg aattacgcag
      901 gtaggtacgc agtttttcaa ttttgatgga aagcagtgtg tccaccagtt tgctgtagct
      961 gcggttgggg tcattacgca tcagatacgc cgcttccgca aaaatggtac gtcctgaacc
     1021 ctgccagaac gggtcctctt ttgtgcccat cgggatcaga gtatttgcgg tattatcaaa
     1081 atccggctgt gtcaggcact ccttccacaa atcccaggcg gcacagcgtg caccggggcg
     1141 gttcagtagt gaaaccggct ctcagtattg ggggcttata aaggcttgag cggagtgcgg
     1201 ggaaactcgc acgctccgtt cttagggggc ggcggcgcag caatgtgccg ctgctacccg
     1261 acccgccagg gcggagacag cttcgctggc tccgttcacg acagccagcc ccgccaaggg
     1321 gacgcccggg aaagccataa acaggagagg agaggaacgg aaagaagaag aggaagaaga
     1381 acaataacag gcacggtaac cacagaaaaa taacatcaga aaaaaatatt ccggacacat
     1441 cataaacggg aagggtaacg ggcgacgcgt cagcgtggcc cgcttccctt ccggcaggcg
     1501 tcagcctgcc aggggttttc gcgcgggctg acaggcggcc tccaccgcct gccctgtatc
     1561 acctgtgcct gctgccctga aggatgtagt gaccaccgag gaaaaccgcc tgctgattcg
     1621 ccacaaaatc gcagatttca tcaacaatct gcgcggtggt gagcggtttc tgccccattt
     1681 cacgacgccg cgcattaatc acggccctgt aattacgcag aatttcgcgg gtttctgtgc
     1741 tgatattcat gacatgtgct ccccgtccag cgcctgtaac acaacctcca ttgcttcgcg
     1801 ttcctccctg acgaatgcgt caccgtcgcc cccgttctca ctcccgacgg catacaggcg
     1861 cttcatttca ctgcctgcca gcgccatcac tttttccagc atcaccgcag ggactgttac
     1921 aagacggttt acgcgctttt cctccatcag ttcattgaca atatcggaaa caactctgtc
     1981 acatgcgcca tatacaaaag catcgtccag ccgctgcatg acggtttcca gctcatcgtc
     2041 tgtcagactc cagtcctggg ctatgtgacg cacatcccca cgggtcagca ccacggcaaa
     2101 aacaaattca ctgtccggca cgtctgtatc cagtacatcg cggataattt ctttaacagt
     2161 acagtacatg ttctcgtctc ctttacaggg gcggcattgc cgccccgtcc gttaatcagt
     2221 ccagtgccgc gtaaatcgcg ttgcattcag ggtgaaatac gatgtgacgc cacaactgcg
     2281 catcgcgcat ccggtaaagc tgggtcagtc ctgcatcccc gctgtcgtgg tacagccaca
     2341 actggcggtt aatcacaaga gaagtcagaa tgatgcctgc ggcatcagca ctcacggtac
     2401 ggtcaaacca gtttgcaccg ttcaccatgt ggaaacggtc gccatccggc atcatgtaac
     2461 cgccaccgtc cggcaggcgg atgaagtgcc agaagccgcc ttcatattcc ggcatcattt
     2521 ttttcgccag cgcgtacaca tacgcctcgg catacagaaa atcgttgcca aacaggtaag
     2581 gcagaaagga gagacggtcg cactcttcaa cattcagagt gacaggtttc gcatattgca
     2641 tgatgttttc ctccgttgtt cacatattcc gcgacaccgg gcggtgccgt gcctccataa
     2701 aagccgggcg ataaggcggg gatgcacagg gcgcaaggga agccgcagcc tgacgcacac
     2761 ggctggcggg agttggaggg tgagaccgcg aaacctgagc gggtgaacga ccggaaaatc
     2821 acggcagacg ggaaggaggg accgggtgac cgcagcggcg aagcgtaccc ttgcatcccc
     2881 gccagcccgg ctaagatgga ggcacggcac agcccgcaca cgacgctgag gctacagagc
     2941 tgaaaaccca caaggccatg ccccgcatgg ccgggtaaag cggagccggc ggcgacttcc
     3001 tttgtgctct gtccggggac cggcaggaaa aaggcgggga tttaccccat aagcgctaac
     3061 ttaagggttg aaccatctga agaacgcgac gcctcggtgc ctcgttaaga cgatgcctcg
     3121 cgttcttcaa ttgcgttttg taggctgtca gggatactgt cccacgaatg gccacctgta
     3181 agctccagat gaccattttt gttattctcc acaacgagtt agttcttctt ttcggatccg
     3241 gcacttctgg ggggaaaatc cagcgatggc tggattatgt cgtcaattaa aaatgcggcg
     3301 agtagattag caaatatcca cgctttcgcg agttcaggtt cctttgcacg caaagcatcc
     3361 aagtgcagca aacttttgag ccgcttaaaa gccagttcaa tttgccatcg cagacggtaa
     3421 caatcagcca cttgctctgc tgaatattca tcttccgata atgatgttag caatagcaca
     3481 tggcccgctg cttccagcgt ttccgcctga actactcgtc cttttcgacg attctcgctg
     3541 agcagtcggg ttttactgat taatgctttt tcgggaggaa gtgatacggc aatgagacgt
     3601 gcaggaaagg gagctccggc ttttttatta cctgaattgc ctatcattac agtggtttca
     3661 ccgttcttac cgcaatccag cccgcgcaga aaatccatca tgtcaaagcg cattccttct
     3721 gcagttaacc agcgcaatcc tcgccagtaa acccggacga tataatcagc ttctccaaaa
     3781 gcaagtgagc ggatacattc gggacgcgaa ccgaatcccc ggtcagcaat tcgtatctcg
     3841 tctgccgttt gcgcaaatcg atccagccgt tcagcgtctc tgctgtcggt tagctcaaaa
     3901 tcagtgaact gacaggtatg aggatcatat cccatatgta gtcgccattc agcgctgccg
     3961 cccccgggcg cgctgattgc tgttccatcg acaagacgca atcaaggtgc ggcgggagtc
     4021 gatggcatgc gcgtaagtga actgccggac tacctgaggc accactggcc agagctgaaa
     4081 gcgcaactgc tgtccggcag ataccgccca tcccctgtga gaagagtatc catactgaag
     4141 cccggcggcg gcgaacgtct gctgggcatc cagatggtgg tggatcgctt catccagcag
     4201 gctatgatgc aggtattgca ggcactgtga gatagctcgt tcagcgacaa cagctacggg
     4261 ttccgtcccg gtcgttcggc ccaccaggca gtgatacagg cccaggaata tatcggggcc
     4321 gggtatcact gggtggtcga cctcgatctg gagaagttct tcgaccggat aaatcacgac
     4381 gtgctgatga gccggataga gaaacgagta tcggataaac gggtgttgtc acttatccgc
     4441 aggttcctga atgcaggggt gatggacgca ggtctggtaa ggccggtgac agaaggaacg
     4501 ccgcagggcg gcgtgatctc gcccctgctc tcaaatctgt tcctgcacta tgcctttgat
     4561 atgtggatgc aacgtcagtg tcccgacgtt cccttcgaaa ggtacgccga tgatagtgtg
     4621 ccacgaaggc gcgcaccagg gacgtgagtc cctgtgcagc catgctgcac aagagatgag
     4681 ggaaggcccc tcggtgtcgg cgtg
//
LOCUS       DADLSA010000230         4691 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.231, whole genome shotgun sequence.
ACCESSION   DADLSA010000230 DADLSA010000000
VERSION     DADLSA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.231"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            226..1248
                     /locus_tag="JN691_004000"
     CDS             226..1248
                     /locus_tag="JN691_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HAY7245260.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1248..2228
                     /locus_tag="JN691_004001"
     CDS             1248..2228
                     /locus_tag="JN691_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAY7245261.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2225..2983
                     /locus_tag="JN691_004002"
                     /pseudo
     CDS             2225..2983
                     /locus_tag="JN691_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2993..3802
                     /locus_tag="JN691_004003"
     CDS             2993..3802
                     /locus_tag="JN691_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAY7245262.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            3802..4656
                     /locus_tag="JN691_004004"
     CDS             3802..4656
                     /locus_tag="JN691_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HAY7245263.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
BASE COUNT         1057 a         1280 c         1181 g         1173 t
ORIGIN      
        1 gacgataaaa aacaataatg tcattcatat ttaaatgatt tagccttaaa tgatttaaat
       61 aatattagcc acctgataaa tacattttta atgatagaga gggtcaacag gcttgcggaa
      121 gtcgtcacat cttcatgttt tcaatagcta aactttgata ttgaacatga tttttatcga
      181 tatatatgaa agtatatatc gattttgctt ttcaggatgt tcaccatgcc cataaccaga
      241 cggacgtttg cgcaggcact tgcatctacc ctgctcctgc agtctctacc gtccttttca
      301 caaacagtta atcgttttgc ttcgcaatct cttcccgaag cccaaaacat tacacgcatc
      361 gtcagcgcag gcgcgcccgc cgatttgcta ttgctggctg tcgcgccaga aaaaatggtc
      421 ggtttttcct cttttgattt tgcccgtcag gcattaattc cattgccaga gcacattcgc
      481 cagttaccca ggctgggacg actcgccggg cgcgccagca cactatcgct ggaagggctt
      541 atggcgttac atcccgattt ggtcgttgat tgcggcaata cggatgaaac cttgatctcc
      601 caggcacggc aggttagcga acagacacaa atcccctggt tattgcttaa cggaaaactg
      661 gcgcaatcag cagaacagtt aacaacgctt ggcaaaacgt taggcgaaga gcaccgcgcc
      721 gcagaacaag ccaatctcgc cagccacttc gttggtgaag ctcaggcatt cgccacctca
      781 cccgccgcta acctccgctt ttatgctgcg cgcggtcccc gagggctgga aacgggctta
      841 cagggttcgt tgcataccga ggcggcggaa ttattaggtt tgcacaacgt cgcgcaaata
      901 gccgatcgcc acggcctgac acaggtttcc atggaaaatc tcctgcgctg gcagccggat
      961 attattctgg ttcaggaggc cgttactgca gattttattc gtcgtgatcc tctctggcag
     1021 ggcgtgaaag ccgttgcgga acaacgcatc ttatttttaa gtggcttgcc ctttggctgg
     1081 ctcgatgccc cgccgggaat caaccgtctt ctgggattac gcagacttca cgcctggctg
     1141 gatcccgcca tcaatcgcca gtttaaaagt gacatgcagc attacgccca actgttctgg
     1201 cattgttcac tcagtgatgc cgactatcaa aaattggtgg cgagctaatg agaatcgtaa
     1261 acggatgtat tttgctgctg gcagctatca gtatcacttt tgccgccgta agtggggctt
     1321 atcatctcga tatgcagcaa ctgctggcgc taattcttcg tcaagaaaat gtgtctgtgc
     1381 aggaacaaat tgtcttctgg caaatccgcg tgccgcgtat tcttgctgcg ctgttactgg
     1441 gcgcagcgct ggcaggagcc ggaaccacct atcagggaat gttgcgtaat cccttagttt
     1501 caccggatat tttaggcgtc tccgccggtg ccggacttgg tgcctgcgct gcaatcctgt
     1561 ggggattacc cattgtttac atccagctgt acgcattttg cggcgggctg atggtggtcg
     1621 cgggcgtctg gttgattacg cgtcgggtga cccgccacga tccgatctta accctggtgc
     1681 tggtaggcat tgcgttagga acgttgtgcg gagcggggat atcgctaatt aaaacgctgg
     1741 ccgatcctta cacccagtta ccgtcaatta ccttctggtt gctgggggga ctgtccaccg
     1801 tgaccttacg cgatctctgc tatgctgcac cgatcattct cataggctcc ttgcccctgt
     1861 tttttctccg ctggcgcatg aatttactca cgctttccga cgacgaagcc cgttcgctgg
     1921 gactgaacgt gacgcgtctg cgctttggat tgattgtctg cgccacacta atcaccgcca
     1981 gcacggtagc aatagcgggc ataattggtt ggatcggtct ggtcgttccg cacattgccc
     2041 gcttgttgac cggtcataac catcagcagc tgttgcctat ggcaatgtgt actggcgcta
     2101 ttctgctcct gcttaccgat acgctggcgc gcagcattgg aacaacagaa atcccacttg
     2161 gcattctgac cgcttttgtt ggcgcaccgt ttttcctgct tttgttgtta cgcggaggtc
     2221 gccagtgact ttgcttgccg ttcgtcatgc gtctctgggt tactcccgcc atccagtgct
     2281 acgcgacgtg tccttcaccc tgtcgcaagg aacaatttgt tgtctgcttg gcgcgaatgg
     2341 ttgcggcaaa accacactca tgcgctccat tcttggcgtt ataccgttat taaaaggcga
     2401 gatcctactg gatagcctcc ctgtgcagac actcagccat cgccagcgcg cacaggctat
     2461 tgcctaggtg ccgcaggcgc acgacggtat ttttgctttc agtgtgctgg atatggtgtt
     2521 gatgggcctg gcccccacca taggcgcttt ttccgttccc ggtaagcaag aacgcctgaa
     2581 agccacggag caactggaaa agctgggcat tttgcatctg gcgacacgac gctggaatac
     2641 gcttagcggc ggtgaacgtc agctggcttt aatcgcccgt gcactcgtcc aacaaccgcg
     2701 cttgttgtta ctggatgagc ccgcctccag cctcgatttc ggtcatcaga tccaacttct
     2761 ggacacgctg gcgcagctca aaaataacgg catgacaatg ttaatgtcga ctcaccatcc
     2821 gctccatgcc aacgccattg ccgacagcat tatccaggta gagccagatg gacgagtaac
     2881 acaaggctta ccgaccgaac agttaacaac gaacaaactg gcggccctgt atcgggtttc
     2941 tgccgaccaa attcatcatc acctctctgc aattagccgc taaggaacaa tcatgctcat
     3001 tgatgatatt gattttgcag acctttatct gcagcaactc aaactggctc accgcacaga
     3061 aaaaacgccc gatcactggg atcaacgggc ggagaaaatg gcggaaaatt gcgcctcgcc
     3121 aacggacagc tacctgcaac aactgatcgc taaaattgac ctgcaaggcg ctcagacctt
     3181 attcgatatg ggctgcggac caggcaccgt atcgctggcg ctggctgaca aactcactac
     3241 ggtttatggc gttgattaca gtcaggggat gctgaacgtt gccgcccgcc gcgccgccgc
     3301 actgaaggct gacaatgtcc actggatcca gcgcgcctgg gaagaggact ggagtgacct
     3361 gccacgctgc gatattgccg tggcttcccg ctccactctg gtggctgata tgcgccatgc
     3421 aatgagcaaa ctgaataatc aggcacggct tcgcgtctat acaactcatc tggtcagcac
     3481 ctcgttcgtc tctccagcca ttcagcgggc ggctggtcgg gaagtgatcg aattacccaa
     3541 ctatatcttt gcgctcaacg tcctgtatca gatgggaatt tatgcccacg ttgattttat
     3601 ccgtggacaa aattgccaac aggataacag cacctgggaa cgttttgagc agaacgtcag
     3661 ctggagtctt ggcgcgctta acgacgatga gcgtgagcgg ttatatcact ggtatcaaca
     3721 acaagatgct cgtgccctcg cccctgccag ccgcgactgg gcgctcatct ggtgggatag
     3781 cgtaccgcag gaggcactgt gatgattttc ctttctcagg cacaaatcga tgcgttactg
     3841 ctggaagata tccagggagg tgacctgacc acgcgagcat taaatattgg acaccagcct
     3901 ggctatatag cgttttttca ccgtcagggc ggctgcgtca gcggcgtttc tgtcgcgtgt
     3961 aagatgttaa ctacgctggg attaactatt gatgacgcgg tcagcgacgg ttcacaagcg
     4021 aacgcgggtc aacggctaat ccgtgcgcag ggtaatgccg cagcacttca tcagggatgg
     4081 aaggcagtcc agaatgtgct ggagtggagt tgcggtgttt ctgattatct cgctcaaatg
     4141 ctggcattgc ttcgtgaacg ttatcctgac ggcaatatcg cctgcacccg aaaagcaatt
     4201 ccgggcaccc gcttactggc ttcgcaggca attctggctg ccggaggact aattcatcgt
     4261 gccggatgtg cggaaaccat attattgttt gccaaccacc gccattttct tcatgacaat
     4321 cagggctggt caggcgcaat cgctcagtta cgtcgccacg cacccgagaa gaaaattgtt
     4381 gttgaagccg acacgccgaa agaggctatc gccgcgttac gcgcacaacc agacgtactt
     4441 cagctcgaca aatttagtcc gcaacaggca actgaaattg cgcaaatagc gccgtcgctg
     4501 gctcctcact gcacgctggc tctgaccggc ggaattaatc tgacgacact caaaaattac
     4561 ctcgactgcg gcattcgcct ttttattacc tccgcgcctt attacgcggc accagctgac
     4621 attaaagtca gtctgcaacc cacagcctct atttaattct ttgttaaacg gaatttattc
     4681 tatgagactc a
//
LOCUS       DADLSA010000231         4616 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.232, whole genome shotgun sequence.
ACCESSION   DADLSA010000231 DADLSA010000000
VERSION     DADLSA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.232"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            20..178
                     /locus_tag="JN691_004005"
                     /pseudo
     CDS             20..178
                     /locus_tag="JN691_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(234..1169)
                     /locus_tag="JN691_004006"
     CDS             complement(234..1169)
                     /locus_tag="JN691_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7245264.1"
                     /translation="MLPNRILFSLMLIVSSGVVASKKAHELYDSIYGGKSAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGSIYYD
                     GEQVRVDYAKAYALFNQAAQQGMNLAWSRLGMMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVEDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKKINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(1180..1413)
                     /locus_tag="JN691_004007"
     CDS             complement(1180..1413)
                     /locus_tag="JN691_004007"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245265.1"
                     /translation="MSKEYTLSVPPFSLNTLGVDMYNNSSHIRKSYIDIPMYNRNLCD
                     FHDLTYSIKSATAILNGKQRDLLKEEHIQVQDK"
     gene            complement(1406..2680)
                     /locus_tag="JN691_004008"
     CDS             complement(1406..2680)
                     /locus_tag="JN691_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7245266.1"
                     /translation="MWSGKVILLSLGLSLASFCHASTDIDQLYERIVNKDQTALKELQ
                     KLADTNNTQALVLLGFIYEHGVTVSVDIPKAIQWYEKACNQGGEYGCYNARYFFQYGK
                     GVTLDVTRAQKYASKSKTDDLNIPPDLMNKIINQAYELKSGAETDKTKRASLIEFVSR
                     YVGQAPENDLLFWGRIGFSKPEILRLATLWAQEGDPEMDYIVGNLYLDDFSPSDDRRS
                     LDTIKKALQWFRTAAEKGYPMAQYTMGDTYENGSAGLKIDAVEAKKWYELAANKNDEK
                     ALVALGNIYYSGLTGEVDYSKASMLFDKAEQQGAKKAALWLSWMYYNGLGETLDCDKV
                     WDYYEKGAGKYSMKFNKDEYLTRCNQDQKTRETQGDTLPYITLKHYGTYGFNANEKPK
                     ICNVSIEINADKISSITNLQLTLELKGGGGSE"
     gene            3073..4443
                     /locus_tag="JN691_004009"
     CDS             3073..4443
                     /locus_tag="JN691_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7245267.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQRVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPENVMADEKLEKCLLTTPGVKDVLLAKLEHCVYVKVDSKITNRFEIERV
                     INEVSY"
BASE COUNT         1229 a         1074 c          938 g         1375 t
ORIGIN      
        1 tttttgaaca tgtaaaaaaa ttcaccaggc attaccaatt aaaggggttt atcttgatgc
       61 tgaccctgcc gccattaact tttatgctcg tctcggcttt gttcagcttt cagcgacacc
      121 aaatgctttt ggtgctgtac ctatgttttt ggcgattcag catattctcg cggcttagat
      181 tattatcgcc acccgctagg gcaagttttg ccttagccgg gggctgagca gcgttaccac
      241 ttttgttcaa ttgcccccgc tttcagcaac tccacatcct tgccgttaat agttgccgtt
      301 gcggcaacga ttgtggtcct tacagttgca caagattttc gctcaaattc cggtttataa
      361 agaataagag acgaattact ggtaaatgaa ccaaataggt aatcagtaaa gctaatattc
      421 agccggttta aaccgaaagg cgcgaagcca accgtttggt taatcttctt accggatggg
      481 cgacgaatgc tgaatgtcac gcgaagattg gccacttcgc ctaatttatt ggtggtggcg
      541 aagaggctat ccatgcagga aaaaccttta tctaaaaaat tatcccgcat cccagaacgg
      601 gtaaccgtga tcacgggtaa cgtatcctca accgtttttc tgtcaatcat gtcttttcga
      661 caagtagcca gaaagtcttc cgggccacca taaatgtgga cacctttatc aaggtattcc
      721 ttcgctttct tacaatctac ctcaacatac tgaccattgg cgtacatcat gcccaacctg
      781 gaccatgcta aattcatacc ttgctgcgcg gcctgattaa atagtgcata cgctttggca
      841 taatccaccc gcacctgctc accgtcgtag tagatactcc ccagcgctaa cggagcttct
      901 ctgtcacctt gtttagcggc taactcgaac catttaatcg cttcctgaac atcaggtttg
      961 gtatcatagc gaggctgata atattcccag cctaacaggc tctgcgcgtc gatatctccc
     1021 gattcagcca ttttatgcag tgtatttata acatcgggag cagatttacc accgtatatg
     1081 ctgtcataca attcatgcgc tttcttactt gccaccacgc ctgatgaaac tatcagcatt
     1141 aaagaaaata atattctatt aggcaacata aaatatctct tatttatctt gtacctgaat
     1201 atgctcttct tttaataaat cacgttgttt tccattcagt atcgctgtcg cagatttaat
     1261 agaataagtc aaatcatgaa aatcgcataa attacgatta tacattggga tgtctatata
     1321 gctttttctt atatgactgg aattattata catatccaca ccgagcgtat tcaaactaaa
     1381 aggagggaca gaaagagtat attctttact cactccctcc cccccctttt agctcaagcg
     1441 tcaactgcaa attagttata ctactaattt tatctgcatt aatttcaata ctgacattgc
     1501 atattttggg tttttcattt gcgttaaagc cataggtccc gtaatgtttt aacgtgatgt
     1561 aaggtaaagt atcaccctgt gtttctcggg ttttctgatc ttgattacaa cgggttaaat
     1621 attcatcctt attgaatttc attgaatatt tccccgcacc tttttcgtaa taatcccata
     1681 ccttatcaca atcaagcgtt tcacctaatc cgttgtaata catccaactt aaccacaatg
     1741 cggctttttt tgccccttgc tgttcagcct tgtcaaaaag catgctcgct ttagaataat
     1801 caacctcacc ggtcaaacca gaatagtata tattccccag ggccactaat gctttttcgt
     1861 cattcttatt tgcagctaat tcgtaccatt ttttggcttc gacagcatca atctttaaac
     1921 cagcactgcc attttcgtag gtatcgccca tggtatactg cgccatcgga taaccttttt
     1981 ctgccgcagt acgaaaccat tgcagtgctt tctttatggt gtctaaagaa cgtctatcat
     2041 cagaaggtga aaaatcatcg agataaagat ttccgacaat ataatccatt tcggggtcgc
     2101 cttcctgcgc ccatagagtc gccagacgca gaatttcagg tttactgaaa ccaattcgcc
     2161 cccaaaaaag caaatcattc tcgggtgcct gaccgacata acgactgaca aattcaatta
     2221 aactcgcccg tttcgttttg tctgtctcag ccccggattt caattcatag gcctgattga
     2281 ttattttatt catcaaatca ggcggaatat taagatcgtc agttttcgac ttactggcat
     2341 acttctgtgc tcgtgtcaca tccagggtga cgcctttacc atattgaaag aaataacgtg
     2401 cattataaca accgtattcg cccccctggt tacaggcttt ctcataccac tgaatcgcct
     2461 ttgggatgtc aacagatact gtcactccgt gttcataaat aaatcccagt agcactagcg
     2521 cctgcgtgtt attggtatca gctaattttt gcagttcttt taacgccgtc tgatctttat
     2581 tgacaatacg ttcatacagt tgatcaatat ctgttgaagc atgacaaaaa gaagccagag
     2641 ataaacccaa tgataataaa ataactttac cagaccacat acattcctcc ttgatatatt
     2701 caaccaccaa acatccacct catgaataaa tacgctgcca taacggccca ggtaataagt
     2761 aaacatttaa gctgaaacag cataaccagt gcctccaacg cttctattta ttgcgataaa
     2821 ataacaccat cctttgataa aaagtcacag cccatcaata aatccaaaaa taatgtcatt
     2881 agttgcagga taaaatcatt tgagcttttc atttgttata tttaacgcat gatgtattaa
     2941 tggcaagaat atgagagaaa ctcatacctg acgtaaaaat cactctctta tgtaatattt
     3001 acttattaac actctgttta cctaacatgc gctccgcact acactatgtg ggcaattttt
     3061 taagaggtgg taatgaacga ttataaaatg acgccaggtg agcggcgcgc gacctggggt
     3121 ttagggaccg tattttcatt gcgcatgctg ggcatgttta tggtactgcc ggttctgact
     3181 acgtacggta tggcactgca aggcgccagc gaagcattaa tcggtattgc cattggtatt
     3241 tatggtctga cccaggccgt ttttcagatt ccgtttggcc tgctttccga ccgcattggt
     3301 cgaaaaccat taattgtcgg tgggctggca gtgtttgccg ccggtagcgt tatcgctgcg
     3361 ctttctgact ccatctgggg aattattctg ggccgggcgc tacaaggctc cggtgcgatt
     3421 gccgctgccg ttatggcgct gctttccgat ctcacgcgcg aacaaaaccg taccaaagca
     3481 atggcgttta tcggcgtgag ctttggcatt acctttgcca ttgcgatggt gcttggcccg
     3541 atcatcactc acaaacttgg gctgcacgcg ctgttctgga tgatcgctat tctggcaacg
     3601 accggcattg cgttgaccat ttgggttgtg cccaacagta gcactcacgt acttaatcgt
     3661 gagtccggaa tggtgaaagg cagtttcagt aaagtgctgg cggaaccgcg gctgctgaaa
     3721 ctcaactttg gcattatgtg tctgcatatt ttgctgatgt cgacgtttgt tgccctgccc
     3781 ggacaactgg ctgatgcggg gttcccggcg gctgaacact ggaaggtcta tctggcgaca
     3841 atgctaatcg cctttggctc ggtcgtgcct ttcattatct acgctgaagt taagcgcaaa
     3901 atgaagcaag tctttgtctt ctgtgtcggg ttgatcgtgg ttgcggaaat tgtgttgtgg
     3961 aacgcgcaaa cgcagttctg gcaactggtg gtcggcgtgc agcttttctt tgtggcgttt
     4021 aatttgatgg aagccctgct gccctcactt atcagtaaag agtcgccagc aggttacaaa
     4081 ggtacggcga tgggtgttta ctccaccagc cagtttcttg gcgtggcgat tggcggttcg
     4141 ctgggcggct ggattgacgg catgtttgac ggtcagcggg tatttctcgc tggcgcaatg
     4201 ctggccgcag tgtggctggc agtcgccagt accatgaaag aaccgccgta tgtcagcagt
     4261 ttgcgcattg aaatcccaga aaacgtaatg gcagatgaaa aactggaaaa atgcttactg
     4321 acaacgcctg gtgtcaaaga tgtactgctg gcgaagttag aacattgcgt ctatgtgaaa
     4381 gtagacagta aaatcaccaa tcgttttgaa atagagcgag taatcaacga agtatcttat
     4441 taaataataa gcacggtgct attcatgaaa aataaaatag caccgtactt cgtaacacca
     4501 taatccttta aaccctccat tattaatact tcgcttaatt ccatataact ggacgattac
     4561 attagaagta aactatactt tttatgaaat atcagggata aattattcac atggaa
//
LOCUS       DADLSA010000232         4449 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.234, whole genome shotgun sequence.
ACCESSION   DADLSA010000232 DADLSA010000000
VERSION     DADLSA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.234"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..1229)
                     /locus_tag="JN691_004010"
     CDS             complement(<1..1229)
                     /locus_tag="JN691_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7245268.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNVGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRS"
     gene            1446..2369
                     /locus_tag="JN691_004011"
     CDS             1446..2369
                     /locus_tag="JN691_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7245269.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(2407..4047)
                     /gene="trg"
                     /locus_tag="JN691_004012"
     CDS             complement(2407..4047)
                     /gene="trg"
                     /locus_tag="JN691_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HAY7245270.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRNRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
BASE COUNT         1075 a         1170 c         1045 g         1159 t
ORIGIN      
        1 tgactgcgtg tctgcgtttc attacccaga tttgactgga agatccccgc cgcgctaacg
       61 ggcaagaaat cttcataggt gatgggttgc gccactaccc aaccacgttc aattaagggc
      121 tgtggatcgt ctccaggatg aatcgcctga cgatgcgcct cacccgaagg cgtcagacgg
      181 taccggaacc atgccaaccc ttgctgacgc attaaaaact cactgtcagg aaaagtgcgg
      241 aaggtttcct gtaaatgcat ttggtgagtg agattatcct gcccggttcc aacgttacgc
      301 agaagatcat catacagttg tcgccctttc ggcgttaatg ccacgccacg ctgctcaatt
      361 tcaccaaagc gcgcggtatg cgtgccctgt ttctgccccg caaacaacac cgtctcttcc
      421 agtgctttaa agctggtctg ccgtagtaaa atcggtacct cgcggcgcgg cggcccctcg
      481 atgagaattt tgggttcaat tccgcattca ggcatcatcg actgcacccg gtcaatatcc
      541 agcgtacgtg gcgtcaggtg gttgatatgg catccaggaa aacagaccac atcagcaatc
      601 aaccgatgtt cgttgtgcaa ggcgcgatag gtttcttcat ctaccgttgc taactggtgc
      661 cagcgaaacg tttccagggc ttcctgcaca aactcctgtg cctgtgtttc gttaaaaccg
      721 ccctgctgct catattcctc taacagttgt cgacaacgtg gggtgaagat atcgcgctga
      781 cgcagaatct ccgccgcttt ctggcgcaaa atttcgttct cgataagctc aaggcggagt
      841 aaggatgtaa aaacgcggaa gggattacgc gccagagaag catcatcaat gggccgaaat
      901 gctgtcgaat gtaccggcac ccctgcctgc gagagatcgt aatagctcac cgggtacatc
      961 cccataatgg caaacatccg ccgaagagta gcaagctctt gtgcagtccc aacgcgaatc
     1021 gccccatgac gttcaacatt cagtcgcgcc agctcgtctg catttaccat tttttcgtgc
     1081 agttgaggat tgttttccag cacagccaga ttcacatcag ctaccagttc cagcagcgtg
     1141 ccatattgcg gaacttcttg ctggtacatg gctgacattg cctgcgaaaa ctgttcccga
     1201 atctcatccg ccgtgatgct gttcgccata gtgtctgcct ccagtggtca gtaatctgga
     1261 gtgtaggtaa ccgcattcac tctggcggga agaatttaca aactgtgatc tcgccgcgaa
     1321 aacatcaata ttatccattt tgctgtaaca taattgcttt aattgttaat aatattttgc
     1381 aatcaagtta tcataatcaa acaacttcac ttgtcagcga caccgcttcg tttttaacat
     1441 cgcttatgga aaaaaatagt ctgtttagtc agcgcatccg tttgcgccac cttcatacat
     1501 tcgtagctgt cgcacaacaa ggaactttgg ggcgcgcggc tgaaaccctt aatttgagtc
     1561 aacctgcgct ctctaataca ttgaatgaac tggagcagct gacgggcgct cgcttgtttg
     1621 agcgtggtcg tcagggggcg caacttacct tacccggcga acaattttta acgcatgcag
     1681 tcagagttct tgacgccatc aacactgccg gacagtcgct tcatcgtaaa gaaggtctta
     1741 ataatgatgt cgtcagggtt ggtgcactac ctactgcggc actggggata ttaccttcgg
     1801 ttataggtca gtttcatcag caacaaaaag agacgacctt gcaagttgcg acaatgagta
     1861 accctatgat tctggcgggt ttaaaaaccg gggaaatcga tatcggcatt ggtcggatgt
     1921 cagatcctga actgatgacc gggcttaatt acgaactgct gtttcttgaa tcgttgaagc
     1981 tggttgtccg ccctaatcac ccgctacttc aggagaacgt aacgctaagc cgggtgctgg
     2041 aatggccggt cgttgtatca ccagaaggca ctgcgccacg ccagcattca gatgcattag
     2101 tgcagagcca gggctgtaaa attccttcgg gttgtatcga aacgctgtct gcttcgctat
     2161 ctcgtcaact taccgttgaa tacgattacg tgtggtttgt cccttctggc gctgtaaaag
     2221 acgacctgcg tcatgccacg ctggtggccc tgcctgttcc gggacatggt gcaggcgaac
     2281 cgattggaat actgacccgc gtagatgcga cgttctcttc tggttgccag ttgatgatta
     2341 acgctattcg aaaatcaatg ccgttctgaa aggtgaaggg atctgtcgat ccctccttga
     2401 acattttcac accgtagcga aactaactgg ttcacccgct ccgcgaggtt ctgccgacac
     2461 agaatgtttg ttcagacgga atacgttcac cgcctcagtt aatcgtgcag cctgttcttc
     2521 aagggacacc gccgctgctg aggcctcttc taccagagaa gcattctgtt gcgtcacctt
     2581 atccatttca gaaatcgcct ggctaacctg agttatgcct ctactttgtt catccgaggc
     2641 ggcggcgatt tcctgcatga tatgtgtgac actcgcgacg gcatcaacaa tagtgctcat
     2701 ggttttccct gccgttgcca cctcatccga ccccaggtca attaacctga ctgattcact
     2761 gatcaagcct tcaatctctt tcgctgcctg agcgctgcgg cttgcgagtg tccgtacttc
     2821 gctggcgaca acggcaaatc cacgcccttg ctcacccgct cgcgcggctt caacggcagc
     2881 attcagtgcc agaatattcg tctggaaagc aatactgttg atgacggcgg tgatctcaga
     2941 aattttcttc gaactcgtgg agatagcgcc catcgttttt actacaccgg aaaccgtctg
     3001 cccgccatcg ctggctttaa tagaagcctc ttgcgccagt ttgctggcat gatgcgcgtt
     3061 atccgcattc tgtttcaccg tcgcagtgag ttgctccatg ctggcggcag tttgttcgat
     3121 agccgccgct tgttcttcgg tgcgagatga cagatccgca ttgccagctg aaatttcgct
     3181 ggtgccacga taaatctctt ccgcgccctg tcgaacagtc cctactgtca tccccagtga
     3241 atgctgcatt tgctgtaaat gacgacttaa gcgaccgatt tcattacgac ccgccggttc
     3301 atcattcatc gtcagatcgc cactggcgat tttttcaatc cgttgtgcgg catgttgcag
     3361 tggacgaatg acgatccgac gtagcaccat aaatgttatc agcgtcatga ccagggcaag
     3421 cacaaacgcg ccaatcatga acatcccacc caggcggttg cgctgatggg ccagttccgc
     3481 cagttggttg gctctggtgc tacgtatctt aacggctttg ttcaaaatat cggtataagc
     3541 attatccagc ggtcggatct gctcactttc atgattgata atcgcttcaa acatgccatt
     3601 tttggcatat ttcaacatag gttgcatacc cgtgatataa gcctgaaagc gttgatttaa
     3661 ttcagtgtcg agggcttcat cagcaggtgt tttcaccggt cgattctgat aagcacgata
     3721 accttgctgc gactgtttaa tctccgattc ggcttgcgca atatttcgct tcattgcttc
     3781 catttccgca atacgactcg cagccccggc ctgaatcata ttgatccggg ctgaacgcaa
     3841 aaaatctgaa ctgttcgcta acccggtccg aatctcaatc tccgcagtaa catcacgctg
     3901 atcgcgatcg gcctgccaga ggaaatagcc agccagagca gaacttaatg cgaatagaac
     3961 taagataccg ccaagaatgc aggcaaataa cggaaccagc ctgatgtgat gcaaaaaacc
     4021 taatcgctgt gagggagttg tattcattac cgttgtctct cgtccaggtt tactcctgat
     4081 agaaagtcat cggcacttga cggtaattac ttatgaccaa aaacctcttt gcgtcacatt
     4141 tttacaacaa ttattgcaga gaaaattaat gctattggaa tgaagagagt aacgaatggc
     4201 taaactggct aacccgaatg acgaaaaatt ctggaaaaca gcacgcaatt tcgtgaagtg
     4261 attattttcg tccctaagga aacctcatag gatgcctgcg ggtgcctgag actttctgtt
     4321 ttcaggctaa ccgggaatca gaaagacgaa ggccccgacc gatatttcta tcaactgtac
     4381 tgcacccatt ttgttggacg atgaaatgga atagccccta atatgtcaaa gccaaaatac
     4441 ccttttgaa
//
LOCUS       DADLSA010000233         4354 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.235, whole genome shotgun sequence.
ACCESSION   DADLSA010000233 DADLSA010000000
VERSION     DADLSA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.235"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            105..257
                     /gene="hokA"
                     /locus_tag="JN691_004013"
     CDS             105..257
                     /gene="hokA"
                     /locus_tag="JN691_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAY7245271.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACKLKE"
     gene            complement(444..656)
                     /gene="cspA"
                     /locus_tag="JN691_004014"
     CDS             complement(444..656)
                     /gene="cspA"
                     /locus_tag="JN691_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAY7245272.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(937..1227)
                     /locus_tag="JN691_004015"
     CDS             complement(937..1227)
                     /locus_tag="JN691_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HAY7245273.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1661..2371
                     /locus_tag="JN691_004016"
     CDS             1661..2371
                     /locus_tag="JN691_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAY7245274.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2421..3395)
                     /gene="ghrB"
                     /locus_tag="JN691_004017"
     CDS             complement(2421..3395)
                     /gene="ghrB"
                     /locus_tag="JN691_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAY7245275.1"
                     /translation="MKPSVILYKALPDNLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3499..4158)
                     /locus_tag="JN691_004018"
     CDS             complement(3499..4158)
                     /locus_tag="JN691_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAY7245276.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
BASE COUNT         1071 a         1050 c         1059 g         1174 t
ORIGIN      
        1 taactgtcca actttttggg gtcagtacaa agtgaggcta ccctatgcct gaacaccata
       61 aggttagcct cttactcgtt ggaaatcaac acagggggct gggaatgccg cagaaatata
      121 gattactttc tttaatagtg atttgtttca cgcttttatt tttcacctgg atgataagag
      181 attcactgtg tgaattgcat attaaacagg agagttatga gctggcggcg tttttagcct
      241 gcaaattgaa agagtaagag tcttcggcgg gaaattattc ccgccttact tacggcgttg
      301 cgcattctca ttgcacccac atttattctt cacaaaaata ataatagatt ttattacgcg
      361 atcgattatt tatttcctga aaaaaaataa aaaaatcccc gccaaatggc agggatctta
      421 gattctgtgc ttttaagcag agattacagg ctggttacgt taccagctgc cgggccttta
      481 gcgccgcttt cgatggtgaa ggacactttc tgaccttcgt ccagagattt gtaaccatcg
      541 ttctggatag cagagaagtg tacgaacaca tctttagagc catcgtcagg agtgatgaag
      601 ccgaagcctt tgtcagcgtt gaaccatttt acgataccag tcattttacc ggacatagtg
      661 tattaccttt aataattaag tgtgcctttc ggcgatatgg cgtgctttac agattttgaa
      721 gcgttaaagg aatgtgcact acgaggggta tcaacgataa ctcttgaagg gacttgcctt
      781 actacactgc tttaatggtc tgtacgtcaa accgttgatg tgcattaagc cacgcattgg
      841 cgggtgatgc aacaattatt tttcatattt atgattaatc ggttggaaaa cggtcctgtc
      901 atcaggaccg taaacagcaa taaagtggat aaaagtctat ttcatcaact gcttacttaa
      961 tgccgggttg gcttgaatca aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg
     1021 tctggattcc cattccttta ccatggcgac tgatacgccc aaaacccggg cgaaatcatc
     1081 gatttttaat cctgtccctt ttcgtaactg ctcaatttcg gtacaggacg ttgttctgtg
     1141 cgtcagggta attttctgcg tttcatcttt aaaaacaatc tgttccaggc tgctcaacag
     1201 ctcatgcatt ggatctttat attccattga aaactcctca aatcacactg cgggatcgtg
     1261 aattgcatcg aagctcatta agaatagtcg ggaaaagcag acacagcggg gtgaagtcag
     1321 ggcatgctct ggctatgatt cgattcagcc tgtcatttag cgtgacttat agccacctaa
     1381 cgatctgata aatgccgata tatctcttat cactcctgtt ttgtaaagat ttcgcaaatc
     1441 taaccttctg cgaaggcgta aatctctggc tacaaaaaag gcaacggtac tatcattttt
     1501 taacaatgaa acaacaaatt aaccttgttt cattttctcc atgattttac caggaagatt
     1561 acgtagaaag ggtatcctgc aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt
     1621 gtgtcgttgt gcgctttttt tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg
     1681 ctctcgctgg tttgcgcctc ttgcggcgtt attaatggta gttagcctga gtgggtgttt
     1741 tgataaagaa ggcgatcagc gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg
     1801 tagcggtgaa cgtctaccaa ccctgactgc cgatcagaaa aaacagtttg gtccttttgt
     1861 ctctgattac gcgattctgt atggttattc tcagcaggtg aatcaggcga tggattccgg
     1921 tctgcgcccg gtcgtagaca gcgttaacgc aattcgcgta ccgcaggatt acgttacgca
     1981 aagcggtccg ctgcgtgaaa tgaatggttc gctgggtgtg ctggcgcaac aactgcaaaa
     2041 tgcgaagttg caggccgatg ctgcgcactc tgcgctaaaa cagagcgatg acctgaaacc
     2101 ggtatttgat caggcattca ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt
     2161 gattccggcg gcacaaactt tcacgcaaca actggtgatg gttggagact acattgctca
     2221 gcaaggtact caggtgagct ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc
     2281 aagtgaatat aacaaactga ttgcgccatt accggcacaa catcaggcgt ttaatcaggc
     2341 ctggactaca gcagttactg caacccaata aagagtaaaa gcccgagcga ttatcgggct
     2401 ttacttcacg cagtcgcggc ttagtccgcg acgtgaggat tcacacagtt cttctcaacc
     2461 tttccttgta acgcatcaat caaattatcc acggcacagg cggccatgcc ataacgcgtc
     2521 tcatgggtgg cagatccaat atgcggtact gcgacgacgt tggccattga gagcaacggc
     2581 gaatctacgg acagtggctc ttgttcgaag acatccagcc cggcggcgtg aatttcgcct
     2641 ttctgcaatg ctgcgatcag tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg
     2701 aaaatggcgg aggatttcat tttggcgaat tgttctgcgc caaacagatg atgcgtctca
     2761 tcagttaacg gcaggatcag gcaaacgaaa tctgactctt gtaacagagt atccaaatcg
     2821 cagtagcggg cgttgaagcg ttcttctgct tctttatggt ggcggcgcgc gttatagagg
     2881 atgggcatgt tgaagccaaa gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc
     2941 ccgacaatgc ccagtgtttt atggtgaacg tcagtgccgt accagtccgg gcctatgctc
     3001 gcggtccatt cgcctgcttt tacccgttct gctacctcca caacccgacg agcggtagac
     3061 aacaccagcg ccatcagcgt atcggcgacg gtttctgtta atacggttgg cgtgtgcatc
     3121 agcagaattt ttcgggcggt aagcgcatcg acatcaaaat tgtcatagcc gacggagatc
     3181 gttgatgtgg cacgcagttt cggcattttt tccagcaatg cggcatctac attctcgttt
     3241 gaacccagta aaccttcagc ttctgcaaaa attgctgcat tttgaacgac ggtttgtggg
     3301 ctgaggtttg ccacctggtg aacggtgaaa tgcgcttgca ggcgttgcag taaattatca
     3361 ggtaaggctt tgtagaggat aacggacggc ttcatgcttc tctccattca ctgataaggc
     3421 gatcacctta gcctgaaata tgatgaagga aaactgaaga atgccaggtg atttctgcat
     3481 cacctggcat gaaagggatt acagcgggct taaggtaatt tctacacggc ggttttgcgc
     3541 cttaccttct gcggtgctgt tgctggcgat tgggttagcc gggccaaggc cctgagtacg
     3601 gatgcggctg gcgtccacgc cctgggtgat caacgcgctg gcaacggaat ccgcacgctg
     3661 ctgggagaga cgcatgttca ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt
     3721 aaccgccgtt ttcggatact ctttcagcac cattgccacg ccggtcaggg tgttagcgcc
     3781 tgccggtttc aggggcgcgc tgctgctgtc gaaggtcaca ttgttcggca tattgaggat
     3841 aatgttatcc ccgctgcggg ttacgctaac accagtgccg cgcattttgt cacgcagctt
     3901 cgcttcctgc acatccatgt aataacccac gccgccgccc agagctgcgc ctgctgctgc
     3961 gccaatcagc gcgcctttac cgcgatcttt cttcgaagaa gagagcgcac caatacccgc
     4021 gcccacgaga gagcccagac ctgcgccgat agcagatttg cctgcttcgc gttcgccggt
     4081 gtaagggtta gttgtgcagc cagataccgc cagagcgcca ctcactacgg cggcaataag
     4141 ataaacacgt ttcttcattg ttaatcctta ataacctttt tattctttgc cacgggttcc
     4201 gtggcgggag attatgccgc gtgaacatga agattattcc tgggaatact cggaaatttg
     4261 taagtaatat ttaactgctc aatacatcta acctttcagg agtcttcggt ttggccaact
     4321 catcctcacg atattccgtt ctgactgccg ccca
//
LOCUS       DADLSA010000234         4324 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.236, whole genome shotgun sequence.
ACCESSION   DADLSA010000234 DADLSA010000000
VERSION     DADLSA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.236"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            176..1459
                     /locus_tag="JN691_004019"
     CDS             176..1459
                     /locus_tag="JN691_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HAY7245277.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            1606..3081
                     /locus_tag="JN691_004020"
     CDS             1606..3081
                     /locus_tag="JN691_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7245278.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            3360..>4324
                     /locus_tag="JN691_004021"
     CDS             3360..>4324
                     /locus_tag="JN691_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7245279.1"
                     /translation="MSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVWMNFRLYQNVSRENISDAMTGLYNR"
BASE COUNT         1171 a          973 c          938 g         1242 t
ORIGIN      
        1 tacacccgta aacaggtgcg tttactgtgc gaatggtatt tgcgcgtacg taaatcgtct
       61 taacaaccct ggcccggtac acatgctacc gggcctacaa cgacagcgaa ccgtgggcct
      121 gagaagcggc aacacaggcg tagcatacag ttggcaaatg tagtacgggg ggcatatgac
      181 ctggtttatt gaccggcgtc tgaacggcaa aaacaaaagc atggtgaatc gccagcgttt
      241 tttacgccgt tataaagcgc aaattaaaca gtcgatctcc gaggccatta ataagcgttc
      301 ggtgactgac gtcgacagcg gcgaatccgt atccattccc acggaagata ttagcgaacc
      361 aatgtttcat caggggcgtg gcggtctgcg ccaccgcgtg catccgggca atgaccattt
      421 cgttcagaac gaccgaattg aacgtcccca gggtggcggc ggaggttccg gcagtggtca
      481 gggccaggcc agccaggatg gtgaaggtca ggatgaattt gtctttcaga tttcgaaaga
      541 tgagtatctt gatctgctct ttgaagattt ggccttaccg aatctgaaac aaaaccaaca
      601 acgccagctg accgaatata aaacgcatcg ggcgggttat accgctaacg gcgttccagc
      661 caatatcagc gttgtgcgtt ccttgcagaa ctcactggcg cgacgcacag ccatgacggc
      721 aggcaagcgg cgggaacttc atgcactgga agagaatttg gccatcatca gcaacagtga
      781 acctgcgcag ctgctggaag aggaacgtct gcgcaaagaa attgcagaat tacgtgccaa
      841 aattgaacgc gtccctttta ttgacacctt cgatttacgt tacaagaact acgagaagcg
      901 gcccgatccc tccagccagg cagtgatgtt ttgcctgatg gacgtttccg gttcaatgga
      961 tcaatccact aaagatatgg ctaagcgttt ttatattctg ctgtatctgt tcctcagcag
     1021 aacgtataag aacgtggaag tcgtatacat ccgccatcat acccaggcga aagaagtcga
     1081 tgaacatgag tttttctact cgcaggaaac aggcggcacc attgtttcca gcgccctgaa
     1141 actgatggat gaggtagtga aagagcgtta taacccggca cagtggaata tttacgctgc
     1201 acaagcatcg gacggcgata actgggccga tgactctccg ctttgccatg aaatcctggc
     1261 gaaaaaatta ttacctgttg ttcgttatta cagctatatc gaaattaccc gtcgtgcaca
     1321 tcagacattg tggcgagaat atgagcatct gcaatctact ttcgacaact ttgcgatgca
     1381 gcacatccgc gaccaggatg atatttatcc ggtgttccgt gaactgtttc ataaacaaaa
     1441 tgcaacagct aaagactaaa actatcagcc gggtcattat cgcctggctg atttttagct
     1501 tactgtaaat tatctcattt attacataca ttagcttaca atcgctttaa atatgacagc
     1561 ataaccttta cacaatttag ttccagaaaa caatcattcg gaaaaatgat tcagtcaaca
     1621 cgtatttccc tggggttatt ctttaaatat tttttatcgt taacgaaaat tgatcttggt
     1681 caaaactata tatctctgcc atcaataaaa tccagcactc acattgctct cctttttatg
     1741 gtttctatgg gtacacaaaa attaaaagct caaagctttt ttattttcag tttattgctg
     1801 acgttaattt tattttgcat caccacatta tataacgaaa agacgaatgt aaaactcatc
     1861 ccacaaatga attatctgat ggtcgttgtg gctttgtttt tccttaacgc catcattttt
     1921 cttttcatgt taatgaaata tttcactaac aaacaaattc taccaacact cattttaagt
     1981 cttgcatttt taagtggcct tatctattta gttgaaacta ttgttgttat ccataaacct
     2041 atcagcgaca gtacactgat ccagacaaag tcgaatgatg tttctatttt ctatattttc
     2101 cgccaactca gttttatttg tttaacctcg ttggcgctct tttgttatgg taaagacaac
     2161 atccttgaca acaataagaa aaaaactggc attctgttgc tggcgctgat tcctttttta
     2221 gtttttcccc ttctggcaca caatctgagc agttataacg ctgactattc tttgtatgtc
     2281 gtcgattact gtcctgacaa ccatactgcg acctggggaa tcaactatac aaaaatattg
     2341 gtttgtctgt gggcattttt actgttcttt attatcatgc gtacacgatt agccagcgaa
     2401 ctatggccat taatagcatt attatgtctg gcatcgctat gctgcaactt acttctactg
     2461 actctggatg agtataatta caccatctgg tacatcagtc gcgggattga agtctccagt
     2521 aaactgtttg ttgtgtcttt tctgatttat aacatttttc aggaactgca actctccagc
     2581 aaactggcag ttcatgatgt gctgaccaat atttataatc ggcgctactt tttcaacagc
     2641 gtagagtcat tattgtcgcg acctgttgtt aaggacttct gtgtcatgct ggttgatatt
     2701 aatcagttca aacgcatcaa tgcccaatgg ggacatcgtg tgggtgataa agtgctggtt
     2761 tcaattgtcg atattatcca gcaaagcatc cgccccgatg atattttagc gcgactggag
     2821 ggtgaggtgt ttggcttgct atttaccgaa ctcaatagtg cccaggcaaa aatcattgcg
     2881 gaacgtatgc gtaaaaatgt cgaactcctg accggcttca gtaacagata tgatgttcct
     2941 gaacaaatga ccatcagtat tggcacggtt ttttcaacgg gtgacacgcg taatatctcg
     3001 cttgtcatga cggaagcaga taaagcctta cgcgaagcga aaagcgaggg gggcaacaaa
     3061 gtgattattc ataatattta agtgcaaaaa tattcagagc catgattttt gcgtggcttt
     3121 tgcatacaat ttattactat tacccctaaa tttcccctcc ccagcggcgc agagatgagt
     3181 ataattagcg cccctgtgcc aggccgcaat cgaactttat ctggttttct cgtttcacta
     3241 accgaaggag tgccatttat catgaaattg caccatagaa tgctccggca ttttatcgcc
     3301 gcaagtgtca ttgtgctgac atcttcctta tttttgaact tgtcgccagc gacagagcaa
     3361 tgagtgccta tctgcgctat atcgtgcaga aagcagactc ctcctttctt tatgataagt
     3421 atcagaatca gagtattgcc gcgcatgtga tgcgcgctct cgctgctgag cagtcggaag
     3481 tgtcgccaga acagcggcgc gccatctgcg aggcttttga gtctgccaat aatacccatg
     3541 gcttaaacct gactgcccat aaatacccgg gcttacgcgg cacactacaa accgcatcca
     3601 ctgactgcga cacaattgtg gaagctgcag cactattacc cgcttttgat caggcagtgg
     3661 aaggcaaccg ccaccaggat gattacggtt caggtcttgg gatggccgaa gagaaatttc
     3721 actattatct cgatctcaat gaccgctatg tctattttta tgagccggtt aatgttgaat
     3781 actttgcgat gaataactgg tccttcctgc agtcaggaag tattggcatc gatcgcaaag
     3841 atattgaaaa ggtatttacc gggcgtaccg tattgtcgag catttaccag gatcagcgta
     3901 ctaaacagaa cgtgatgagt ttgctgacgc cggtatatgt cgcagggcag ctaaaaggga
     3961 ttgtgctgct ggatattaac aaaaacaatt tgcggaatat cttttacact catgaccgcc
     4021 ctctcctctg gcgttttctc aatgtcacgc taaccgatac cgattcgggg cgcgacatta
     4081 tcatcaacca gagcgaagat aatctgttcc agtatgtcag ttacgtccat gacttaccgg
     4141 gcggcattcg tgtctcgtta tccattgata ttctttactt tatcacgtct tcgtggaaaa
     4201 gcgttctgtt ctggattttg acggcgttaa ttttgctgaa tatggtctgg atgaacttcc
     4261 gtttatacca aaatgtgtcg cgagaaaata ttagtgatgc gatgactgga ctgtataatc
     4321 gcaa
//
LOCUS       DADLSA010000235         4313 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.237, whole genome shotgun sequence.
ACCESSION   DADLSA010000235 DADLSA010000000
VERSION     DADLSA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.237"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..767
                     /gene="cheR"
                     /locus_tag="JN691_004022"
     CDS             <1..767
                     /gene="cheR"
                     /locus_tag="JN691_004022"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HAY7245280.1"
                     /translation="LIYQRAGIVLADHKRDMVYNRLVRRLRSLGLTDFGHYLNLLESN
                     QHSGEWQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYRVWSAAASTGEEPYSIA
                     MTLADTLGTVPGRWKVFASDIDTEVLEKARSGIYRHEELKNLTPQQLQRYFMRGTGPH
                     EGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMIYFDQTTQQEILRRFVP
                     LLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD"
     gene            770..1819
                     /locus_tag="JN691_004023"
     CDS             770..1819
                     /locus_tag="JN691_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HAY7245281.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            1834..2223
                     /gene="cheY"
                     /locus_tag="JN691_004024"
     CDS             1834..2223
                     /gene="cheY"
                     /locus_tag="JN691_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheY"
                     /protein_id="HAY7245282.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDSLN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            2234..2878
                     /gene="cheZ"
                     /locus_tag="JN691_004025"
     CDS             2234..2878
                     /gene="cheZ"
                     /locus_tag="JN691_004025"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HAY7245283.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQASERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(3335..3718)
                     /locus_tag="JN691_004026"
                     /pseudo
     CDS             complement(3335..3718)
                     /locus_tag="JN691_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707773.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3687..>4313)
                     /locus_tag="JN691_004027"
     CDS             complement(3687..>4313)
                     /locus_tag="JN691_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HAY7245284.1"
                     /translation="EAKNDTWKEQRNALSPAEINELSMQMFRVYATSALINAVVEAFC
                     EQLPALMQRDCPFKDLISQSKGHALCTALKMFDRTRGYLHRSVLQQELRGSNYITNLM
                     DMLWIGIHGHKTKKQRDGERESASADNLFVSREEYLSDTPFGRYAYGTISENYRRVFE
                     DPANTLPVLYKEAQLLTDAISGMTDSYLMRLHDELKSLYECGLKHPTA"
BASE COUNT         1082 a          981 c         1183 g         1067 t
ORIGIN      
        1 aattgatcta tcaacgagcc gggatcgttc tggctgacca taaacgcgac atggtttaca
       61 accgactggt tcgtcgtttg cgttcgctgg gactgacgga tttcggtcat tatctgaact
      121 tgctggaatc taatcagcac agcggtgagt ggcaggcgtt tatcaattcg ctgaccacga
      181 atctgacggc atttttccgt gaggctcatc atttccctct gctcgcggat cacgcacgtc
      241 gccgttctgg cgagtatcgc gtatggagcg cggcggcttc gaccggcgaa gagccgtaca
      301 gcattgcgat gactctggct gacacattgg gcaccgtgcc cggacgctgg aaagtgtttg
      361 ccagtgatat cgacaccgaa gtgctggaaa aagccagaag cggtatctat cgccatgaag
      421 agttgaaaaa cctgacgccg cagcaactgc aacggtattt catgcgaggg acgggaccgc
      481 atgaagggct ggtacgcgtg cgtcaggagc tggcgaacta tgttgatttt gccccgctga
      541 atttactggc gaaacagtac accgtgccgg ggccgtttga tgcgatcttc tgtcgtaacg
      601 tcatgatcta cttcgatcaa actacccagc aggagatttt gcgccgcttt gttccgctcc
      661 ttaaacccga cggattgctg tttgcgggtc actctgaaaa ctttagccac ctcgagcgcc
      721 gcttcacgct gcgtggtcag acggtgtatg cgctaagtaa ggattaacga tgagcaaaat
      781 cagggtgtta tctgtcgatg attcggcact gatgcgccag atcatgacag aaatcatcaa
      841 cagccatagc gacatggaaa tggtggcgac cgcgcctgat ccgctggttg cgcgtgactt
      901 gattaagaaa ttcaatcccg atgtgctgac gctggatgtt gaaatgccgc ggatggacgg
      961 actggatttc ctcgaaaaat taatgcgttt gcgtccaatg cccgttgtga tggtttcttc
     1021 cttgaccggc aaaggatcag aagtcacgct gcgcgcgctg gagctggggg caatagattt
     1081 tgtcaccaaa ccgcaactgg gtattcgcga aggaatgctg gcgtatagcg aaatgattgc
     1141 tgaaaaggtg cgtacggcag caaaggcgag ccttgcagca cataagccat tgtcagcacc
     1201 gacaacgctg aaggcagggc cgttgttgag ttctgaaaaa ctgattgcga ttggtgcttc
     1261 aacgggtgga actgaggcaa ttcgtcacgt actgcaaccg ttgccgcttt ccagcccggc
     1321 attgttaatt acccaacata tgccgcccgg tttcacccgc tcttttgccg acagacttaa
     1381 taagctttgc cagatcgggg ttaaagaagc cgaagacgga gaacgtgtct tgccggggca
     1441 tgcctatatt gcgccgggcg atcggcatat ggaactggcg cgtagtggcg caaattatca
     1501 aatcaaaatt cacgacggcc cggcggttaa ccgtcatcgg ccttcggtag atgtgttgtt
     1561 ccattctgtc gccaaacagg cggggcgtaa tgcggttggg gtgatcctga ccggtatggg
     1621 caacgacggc gcggcgggaa tgttggcgat gcgtcaggcg ggggcatgga cccttgcgca
     1681 aaacgaagca agttgcgtgg tgttcggcat gccgcgcgag gccatcaata tgggtggtgt
     1741 ctgcgaagtg gtcgatctta gccaggtaag ccagcaaatg ttggcaacaa ttagtgccgg
     1801 acaggcgata cgtatttaaa tcaggagtgt gaaatggcgg ataaagaact taaatttttg
     1861 gttgtggatg acttttccac catgcgacgc atagtgcgta acctgctgaa agagctggga
     1921 ttcaataatg ttgaggaagc ggaagatggt gtcgactctc tcaataagtt gcaggcaggc
     1981 ggttatggat ttgttatctc cgactggaac atgcccaata tggatggcct ggaattgctg
     2041 aaaacaattc gtgcggatgg cgcgatgtcg gcattgccag tgttaatggt gactgcagaa
     2101 gcgaagaaag agaacatcat tgctgcggcg caagcggggg ccagtggcta tgtggtgaag
     2161 ccatttaccg ccgcgacgct ggaggaaaaa ctcaacaaaa tctttgagaa actgggcatg
     2221 tgaggatgcg actatgatgc aaccatcaat caaacctgct gacgagcatt cagctggcga
     2281 tatcattgcg cgcatcggca gcctgacgcg tatgctgcgc gacagtttgc gggaactggg
     2341 gctggatcag gccattgccg aagcggcgga agccatcccc gatgcgcgcg atcgtttgta
     2401 ctatgttgtg cagatgaccg cccaggcttc ggagcgggcg ctgaacagtg ttgaggcgtc
     2461 acaaccgcat caggatcaaa tggagaaatc agcaaaagcg ttaacccaac gttgggatga
     2521 ctggtttgcc gatccgattg accttgccga cgcccgtgaa ctggtaacag atacacgaca
     2581 atttctggca gatgtacccg cgcataccag ctttactaac gcgcaactgc tggaaatcat
     2641 gatggcgcag gattttcagg atctcaccgg gcaggttatt aagcggatga tggatgtcat
     2701 tcaggagatc gaacgccagt tgctgatggt gctgttggaa aacatcccgg aacaggagtc
     2761 gcgtccaaaa cgtgaaaacc agagtttgct taatggacct caggtcgata ccagcaaagc
     2821 cggcgtggta gccagtcagg atcaggtgga cgatttgttg gatagtcttg gattttgatt
     2881 tgtattgcct gaacttcggc ctgcgcgatg atgccaggcc gatagcaggg tggcgggtat
     2941 cgttcaggga gtacgtggag gatggttatt tgtagtttga taattttaga tcagcgaagc
     3001 gtgattgttg cgccagtaaa tttatcaatg cattaaattg tgaacgagaa aatgacgatg
     3061 actggtagtg cagtacatcg tagtgatgcc atacttcata acgttttttc gtcagttcat
     3121 ggtgcaagaa atgacagagt tccggcccta aactctttac ctgcttagta aaaatagtgc
     3181 gtaaaatctt cgttaccgaa ccgcttggat tggggttagt atgaagaaca agttcaagat
     3241 gtctgcgatt taagtcggtc aagtaattat ctttactgat aattttttca ctacgtagat
     3301 catagagcac ggcatcccaa ctcattagcg tagtgtttaa cagacttgat tcatcctcac
     3361 aaaatataag tttttcccgg aatgcccatg tctcattgag gttccagata tcaaattcga
     3421 agtcgtgata acgaaaacgg atcccaccaa acttattaaa acgcagttgt ttgacgggca
     3481 gttcagccaa tgtctggaag agttcctcac gggagcgacc gatgacgata tcaaggtctg
     3541 aagagaatcc atcgacgcca tacaagccta agtcacgcac catcccgcca aaaatccagg
     3601 ccggacagga acaggccaga gtatcaagta atactctggc tttcttatgc tcatcactaa
     3661 gcgtaaaaaa atcggcgata cgctgttcaa gctgttgggt gtttgagacc acattcatac
     3721 agtgatttta gctcatcgtg aaggcgcatt aaataactat ccgtcatgcc tgagattgcg
     3781 tcggtaagca actgcgcttc tttataaaga acagggagtg tattggcggg atcttcaaac
     3841 acgcggcgat aattttcaga tattgtcccg taagcatagc gaccaaaggg agtatccgac
     3901 aggtattctt cacgagaaac aaaaaggtta tctgcagacg cggactcgcg ttctccgtct
     3961 cgttgtttct ttgttttgtg tccatgaatg ccaatccaga gcatatccat caagttagta
     4021 atgtaattag accccctaag ttcctgttgc aagactgaac gatgaaggta gcctcgtgtt
     4081 cggtcgaaca ttttcagggc agtgcagagg gcgtgacctt tgctttgcga gattaaatct
     4141 ttgaacgggc agtcacgctg catcagcgcg ggtaattgtt cgcaaaaggc ttctactacg
     4201 gcattaatca atgcactggt ggcatacact ctgaacattt gcatactgag ttcattaatt
     4261 tccgccggtg aaagtgcatt tctttgctct ttccaggtgt catttttggc ctc
//
LOCUS       DADLSA010000236         4313 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.238, whole genome shotgun sequence.
ACCESSION   DADLSA010000236 DADLSA010000000
VERSION     DADLSA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.238"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..532)
                     /locus_tag="JN691_004028"
     CDS             complement(<1..532)
                     /locus_tag="JN691_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245285.1"
                     /translation="MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGL
                     ALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILS
                     LAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVS
                     ENTKQRNLFSKKMTLCI"
     gene            1047..3470
                     /gene="pgaA"
                     /locus_tag="JN691_004029"
                     /pseudo
     CDS             1047..3470
                     /gene="pgaA"
                     /locus_tag="JN691_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            3479..>4313
                     /gene="pgaB"
                     /locus_tag="JN691_004030"
     CDS             3479..>4313
                     /gene="pgaB"
                     /locus_tag="JN691_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HAY7245286.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPVDEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANSTGSLMPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GL"
BASE COUNT         1271 a          950 c          946 g         1146 t
ORIGIN      
        1 ttatgcacaa agtcattttt ttggaaaata aattgcgctg ttttgtattt tcactaactt
       61 tattcaccaa tgccagaaat attaatatgc acaaactcat ctgacgaaac aaataataga
      121 ttgcaatgtc attttggaca acactgcttg tcaaacgccc ctcaataatt tgctgaatga
      181 taatgacggt tttaacaaag taaatcaaac aactcagaaa agccaaacta agtatacagt
      241 ttgaaaattg cctacggtca taaatgaaat acaactgcat aaaaataaat gcaacagtat
      301 cgataaaaaa taaagcaatc attaccatca gataggttga tcgtggaaca acttcctcaa
      361 cgcctggctg attaaaccaa ctattcacca aaaccaaagc aagcccaaac aataagctca
      421 ctaatacagt actactaatt ctcaaatagt ctttttccat aaagctacac caatctgtag
      481 gttgtagatc cctttctatt attaatagat ataagaactg tactgttgtc aatatgaaat
      541 tccgcccaca aacaataccg atgatgatag ttgctgaatg taattattat tgcctaagtg
      601 ctttatacat tgtttgcaac aagccaagct tgaaaatact tataaatggt tatagaaaat
      661 ccttataaaa cattaattca tagatacatt aggaataaca attaaatccg tgagtgccgt
      721 agcgcagcct ttcatcagga ctttcgaaaa atccgaaatc atgcatcgga atttactgat
      781 ttaattattt taatcctaat ttattttgaa aaaggcattg ggatttatgc cgtattcctg
      841 aagatcctca tcattggaat ggattttcgg gcgagaaaag gattttatat ggacactctg
      901 ctcatcattt cttcttctca tcatcaacaa ttcacgtctc tcttccgcgt ttaataacgg
      961 attatgaggt gcaaaaatat ctttcttttc agttacctgt aattagatac agagagagat
     1021 tttggcaata catggagtaa tacaggatgt attcaagtag cagaaaaagg tgcccgaaaa
     1081 ccaaatgggc tttgaaactt cttactgccg catttttagc agcgagtccc gcggcgaaga
     1141 gtgctgttaa taacgcctat gatgcattga ttattgaagc tcgcaagggt aatactcagc
     1201 cagctttgtc atggtttgca ctaaaatcag cactcagcaa taaccaaatt gctgactggt
     1261 tacagattgc cttatgggcc gggcaagata aacaggttat taccgtttac aaccgctacc
     1321 gtcctcagca attaccagcg cgtggttatg cagctgtcgc cgtcgcttat cgtaacctgc
     1381 aacaatggca aaactcgctt acactgtggc aaaaggcgct ctctctggag ccgcaaaata
     1441 aggattatca acggggacaa attttaaccc tggcagatgc gggtcactat gatactgcgc
     1501 tggttaaact taagcagctt aactctggag caccggacaa agccaattta ctcgcagaag
     1561 cctatatcta taaactggcg gggcgtcatc aggatgaatt acgggcgatg acagagtcat
     1621 tacctgaaaa tgcatctacg caacaatatc ccacagaata cgtgcaggca ttacgtaata
     1681 atcaacttgc tgccgcgatt gacgatgcca atttaacgcc agatattcgc gctgatattc
     1741 atgccgaact ggtcagactg tcgtttatgc ctacgcgcag tgaaagtgaa cgttatgcca
     1801 ttgccgatcg cgccctcgcc caatacgctg cattagaaat tctgtggcac gataacccag
     1861 accgcactgc ccagtaccag cgtattcagg ttgatcatct tggcgcgtta ttaactcgcg
     1921 atcgttataa agacgttatt tctcactatc agcgattaaa aaagacgggg caaattattc
     1981 cgccctaggg gcaatattgg gttgcatcgg cttatctcaa agatcatcag ccgaaaaaag
     2041 cacagtcaat aatgaccgag ctcttttatc acaaggagac cattgccccg gatttatccg
     2101 atgaagaact tgcggatctc ttttacagcc acctggagag tgaaaattat ccgggcgcgc
     2161 taactgtcac ccaacatacc attaatactt cgccgccttt ccttcggtta atgggcacgc
     2221 ctacgagcat cccgaatgat acctggttac aggggcattc gtttctctca accgtagcaa
     2281 aatatagtaa tgatcttcct caggctgaaa tgacagccag agagcttgct tataacgcac
     2341 caggaaatca gggactgcgc attgattacg cgagtgtgtt acaagcccgc ggttggcctc
     2401 gtgcagcaga aaatgaatta aaaaaagcag aagtgatcga gccacgtaat attaatctgg
     2461 aggttgaaca agcctagaca gcattaacgt tacaagaatg gcagcaggca gctgtcttaa
     2521 cgcacgatgt tgtcgaacgt gaaccgcaag atcccggcgt tgtacgttta aaacgtgcgg
     2581 ttgatgtaca taatcttgca gagcttcgta tagctggttc aacaggaatt gatgccgaag
     2641 gcccggacag tggtaaacat gatgtcgact taaccaccat cgtttattca ccaccgctga
     2701 aggataactg gcgcggtttt gctggattcg gttatgccga tggacaattt agcgaaggaa
     2761 aagggattgt tcgcgactgg ctggcgggtg ttgagtggcg gtcacgtaat atctggctcg
     2821 aggcggagta cgctgaacgc tttttcaatc atgagcataa acccggcgcg cgcctgtctg
     2881 gctggtatga ttttaatgat aactggcgta ttggttcgca actggaacgc ctctctcacc
     2941 gcgttccatt acgggcaatg aaaaatggtg ttacaggcaa cagtgctcag gcttatgttc
     3001 gctggtatca aaatgagcgg cgtaagtacg gtgtctcctg tgctttcact gatttttccg
     3061 acagtaacca gcgtcatgaa gtctcaattg agggtcagga acgcatctgg tcttcaccat
     3121 atttgattgt cgatttccta cccagtctgt attacgaaca aaatacagaa cacgataccc
     3181 catactacaa ccctataaaa acgttcgata ttgttccggc atttgaggca agtcatttgt
     3241 tatggcgaag ctatgaaaat agctgggagc aaatcttcag cgcaggtgtt ggtgcctcct
     3301 ggcaaaaaca ttatggcacg gatgtcgtca cccaactcgg ctacgggcaa cgcattagct
     3361 ggaatgacgt gattgatgct ggcgcaacgc tacgctggga aaaacgacct tatgacggtg
     3421 acagagaaca caacttatac gttgaattcg atatgacatt cagattttaa ggataaatat
     3481 gttacgtaat ggaaataaat atctcctgat gctggtgagt ataattatgc tcaccgcgtg
     3541 catcagccag tcaagaacat catttatacc gccacaggat cgcgaatctt tactcgccga
     3601 gcaaccgtgg ccgcataatg gttttgtggc gatttcatgg cataacgttg aagacgaagc
     3661 tgccgaccag cgttttatgt cagtgcggac atcagcactg cgtgaacaat ttgcctggct
     3721 gcgcgagaac ggttatcaac cggtcagtat tgctcaaatt cgtgaagcac atcgaggagg
     3781 aaaaccgcta ccggaaaaag cagtagtgct gacttttgat gacggctacc agagttttta
     3841 tacccgcgtc ttcccaattc ttcaggcctt ccagtggcct gctgtatggg cccccgtcgg
     3901 cagttgggtc gatacgccag tggatgaaca agtaaagttt ggcgatgaga tggtcgatcg
     3961 agaatatttt gccacgtggc aacaagtgcg agaagttgcg cgttcccggc tcgttgaggt
     4021 cgcttctcat acatggaatt ctcactacgg tattcaggct aattccaccg gcagcttaat
     4081 gcctgtatat gtaaatcgtg catattttac tgaccacgca cggtatgaaa ccgcagcaga
     4141 ataccgggaa agaattcgtc tggatgctgt aaaaatgacg gaatacctgc gtacaaaggc
     4201 tgaggtaaat ccacacgttt ttgtttggcc ttatggcgaa gcgaatggca tagcgataga
     4261 ggaattaaaa aaactcggtt atgacatgtt tttcaccctt gaatcaggtt tgg
//
LOCUS       DADLSA010000237         4232 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.239, whole genome shotgun sequence.
ACCESSION   DADLSA010000237 DADLSA010000000
VERSION     DADLSA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.239"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            64..1122
                     /gene="yeeE"
                     /locus_tag="JN691_004031"
     CDS             64..1122
                     /gene="yeeE"
                     /locus_tag="JN691_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAY7245287.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            1136..1363
                     /locus_tag="JN691_004032"
     CDS             1136..1363
                     /locus_tag="JN691_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HAY7245288.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1406..2830)
                     /gene="sbcB"
                     /locus_tag="JN691_004033"
     CDS             complement(1406..2830)
                     /gene="sbcB"
                     /locus_tag="JN691_004033"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAY7245289.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            3012..4190
                     /locus_tag="JN691_004034"
     CDS             3012..4190
                     /locus_tag="JN691_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7245290.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQIKPFLEKAMTTYNDVLFPGEDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTL
                     VTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD
                     "
BASE COUNT         1037 a          989 c         1090 g         1116 t
ORIGIN      
        1 ttggtctgca ttccaccagt catgaaaaat ttccccatta acattcatct ggttaattaa
       61 tttatgtttt caatgatatt aagcgggcta atttgtggtg ctctgctggg atttgtcatg
      121 cagcgtgggc gtttttgcct gactggtggt tttcgtgata tgtatatcgc gaaaaataat
      181 cgcatgtttt acgccttgct gattgcaatt tcggtacaaa gcgtgggggt ttttgcgtta
      241 attcaggcgg gcctactgac ttacgaagcc ggggcgttcc cgtggcttgg tactgttata
      301 ggtgggtatc ttttcgggct gggaattgtt ctggcgggtg gatgtgccac cgggacctgg
      361 tatcgcgcgg gtgagggatt gatcggcagt tggatcgcgc ttttcactta tatggtgatg
      421 agtgcggtga tgcgttctcc acacgccagt ggtttaaatc aaaccttgca gcactacact
      481 actgaacata actctattgc tgagactttt aatttgtctg tgtggccgtt ggttaccgtt
      541 ttgctggtga taacgctctg ggtggtgatg aaagagttga agaagccaaa actcaaagtc
      601 gcgaccttac cgccgcgccg aaccgggatc gctcatattc tgtttgaaaa acgctggcat
      661 ccctttgtca ctgctgtact catcggtttg attgcgcttt tagcctggcc cctgagtgaa
      721 gcaaccggac gcatgtttgg gttaggagtc acttccccaa cggccaatat tctgcaattt
      781 ctggtcgcgg gtgacgtgaa atacattaac tggggcgttt tcctggtgtt agggatcttc
      841 gtgggggcat ttattgcagc caaagcgagc cgtgagttcc gcgttcgcgc agctgatgca
      901 caaacaacat tacgtagcgg gttaggtggt gtactgatgg gcttcggtgc cagtattgca
      961 ggtggttgct ctatcggtaa tggactggtt atgactgcaa tgatgacctg gcagggctgg
     1021 attggccttg tatttatgat tctcggagtc tggactgcgt cctggcttgt gtatgttcga
     1081 ccgcagcgta aggcgcgact ggcaactgct gcggcaaatt aagtaaggtg taaagatggc
     1141 gattaaaaag ctggatgtgg tgacgcaggt ctgtccgttt ccactcatag aagcaaaagc
     1201 agcactggca gaaatggcca gcggcgatga gttggtaatt gagtttgatt gcacccaggc
     1261 aactgaagca atcccacagt gggcggcaga agaaggacat gccatcaccg attatcagca
     1321 gattggcgat gcagcgtgga gcatcaccgt tcaaaaagcc tgactcaaca ttgtcctccg
     1381 ccgtaccagc ggcggcggct tcaaattaga cgatctcttc cgcgtactgc caaagtgctt
     1441 ttaacagcgc cactttctct ttgtcatcgg catattgttg taccagcatc tgcaattcat
     1501 cagcataacc ctgcaaaaac tctggcgtga agacctggcg gcggtgctcc agccagcgtt
     1561 gctgctcggc ataatccagc gtccccggga agttgcgtgc ccgataattg aacaacaact
     1621 tttcaattcg tttatcaaca aaagtgatat ccagtgccgg taaattacgc ggctcggttt
     1681 ccagcacaat tttcattgct gcacgatcgg cgtcactgaa aaagccatta taaagctgtg
     1741 catccacatt atctgaaggt gtaaacggtt cggcttccgc gaatatcgct accacttttt
     1801 cacgcacttg cggattttca cgcagaattt ttaggttatc gaggcaatgc tgacgattaa
     1861 ttcccagtcg gtcggcatct tccgggcgta gcgtattcgc ctgggccagt accggacatt
     1921 tattgatatg caccagctta accggaacgg cggcgttatc gccaagatcg gcttttgcgg
     1981 tatataaacg ctcgcgcaat gtgtcgatat ccagctccag taatggcgaa atgtctcctg
     2041 ccaaatccgc cataattacg gcattgcgat tttcaggatg ccacgccagc ggtgccaccc
     2101 agctggtatt gccacgccat gccccaaaca ttccggaaac gtgagccagg ggtttcatct
     2161 gcggaacatc aatcaacgcc atcagtttgt gtttattacg atgggtaaag agataatcaa
     2221 acaggcgtgg ctgacgcgtt tttaccagtt tcgccatcgc aatagtggcg tacacatcag
     2281 ccatcgcatc gtgggcgttg ctatgttcaa taccattcgc tttggttaaa tgctcaaggc
     2341 gaaagctcgg tagaccgtca tcattttcag gccagtttat tccttccggg cgcagagcat
     2401 aacaggcacg cataacatcc agtaaatccc agcgcgagtt atcatgctgc cagctccagg
     2461 cgtaaggatc gtagaaatta cgataaaaaa cgttgcgtgt gacttcgtcg tcgaaacgca
     2521 cattgttgta gcccagaata caggtcttcg gtacggtaaa aagcgagtga atacgggcgg
     2581 caaacgcggc ttcgttttct cctttcgccc gtgcctcctg cggggtaata ccggtaatta
     2641 atactgctcc aggccggggt aaatagtcat ccgcgggctt gcagtaaaag acttcgggtt
     2701 cgccgatgac attgaattcg tcatcggtgc gaatggctgc gaactgtgca gggcgatcta
     2761 acgcggggtg cgtgccaaag gtttcgtaat cgtgaaagag gaaggtaggt tgtttatctg
     2821 tgtctgtcat tgtctagcat tgtccactgt agttaattaa ctgtctgtta taactcgaaa
     2881 aacaaccctt ttttcacctg agtatgtcta cgaacgtcaa atatagttgt ttactgtgcg
     2941 ctgttgtcca tgtgatattg tgtaccagat gtgtaccaaa ctattatttc tgagcgtacc
     3001 aaatattatt tatggctata agcgacacaa aactgcgtac tatttatggt aaaccatatt
     3061 cgggcccaca agaagtggct gatgccgatg gcctcagcgt acgaatttca ccgaaggggg
     3121 tcatccagtt ccagtaccgc tatcgctggc atggcaagcc taatcgactt gggcttggtc
     3181 gatacccatc cctgtctttg aaggatgcca gacagatcac tgctgacttg cgaaagctct
     3241 atttctcagg aacggatcca cgcacctatt ttgaagagaa ggtggagaac tccatgacgg
     3301 tcgcccagtg tctcgactac tggttcgaca actacgtctc tacaactctc agagaaaaga
     3361 cccaggcact ttatcgatca acggttatga agcgcatgca tgacgccttc cctaatcgtc
     3421 cggcatcttc tatcacggtt aagcaatggg ttgacctgct taccgaagaa gaaaaagata
     3481 atccacgccg agcaaggcag gtgctaagtc aactaagatc agcaattagt tggtgcatgc
     3541 ggcgtcagtt gatagatagt tgcgcaatta tgagcatcca gccaagggac ttcggctccc
     3601 gcgctgaggt aggggatcgg gtactgtcgt atcacgaact ggctaagatt tggcttgcta
     3661 ttgaaagaag ccgtgcgtct acgtcaaata agctccttca tcagatgctt atgctgtggg
     3721 gggcgaggct ctcagagctt aggctggcaa caaagacaga atttgacctg ctggacaacg
     3781 tatggaccgt accgaaagag catagcaaga tgggtaatgt tatccgccgt ccaatcttcg
     3841 aacaaattaa gccttttctc gaaaaggcca tgacaacgta caatgatgtt cttttccctg
     3901 gagaagacat aaacaaaccg atcagcatcg ctgcagccaa ccgattcgta aatagaataa
     3961 ggggagggat ggatctaggt tactggcgaa cacatgattt cagaagaacg cttgttacac
     4021 ggctgtccga gatgaatgtc gagcctcatg ttactgagcg aatgctcggt catgaacttg
     4081 gcgggataat gtccgtatac aataaacacg actggataga ggctcagcgc aaagcgtatg
     4141 agcttcacgc tgataaattg ttctggcaca tcaggagcat ttctgattaa cgccaccgtt
     4201 aagaatccac ccttcaacag cttcacgaag gt
//
LOCUS       DADLSA010000238         4183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.240, whole genome shotgun sequence.
ACCESSION   DADLSA010000238 DADLSA010000000
VERSION     DADLSA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.240"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..209)
                     /locus_tag="JN691_004035"
     CDS             complement(<1..209)
                     /locus_tag="JN691_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7245291.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="JN691_004036"
     tRNA            complement(324..399)
                     /locus_tag="JN691_004036"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="JN691_004037"
     tRNA            complement(406..480)
                     /locus_tag="JN691_004037"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="JN691_004038"
     tRNA            complement(597..681)
                     /locus_tag="JN691_004038"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="JN691_004039"
     tRNA            complement(690..765)
                     /locus_tag="JN691_004039"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="JN691_004040"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="JN691_004040"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAY7245292.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JN691_004041"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JN691_004041"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAY7245293.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JN691_004042"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JN691_004042"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAY7245294.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1079 a         1039 c          932 g         1133 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tag
//
LOCUS       DADLSA010000239         4107 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.241, whole genome shotgun sequence.
ACCESSION   DADLSA010000239 DADLSA010000000
VERSION     DADLSA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.241"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(953..1144)
                     /locus_tag="JN691_004043"
     CDS             complement(953..1144)
                     /locus_tag="JN691_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY7245295.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            1208..1531
                     /locus_tag="JN691_004044"
     CDS             1208..1531
                     /locus_tag="JN691_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAY7245296.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1521..2231
                     /locus_tag="JN691_004045"
                     /pseudo
     CDS             1521..2231
                     /locus_tag="JN691_004045"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2209..2739
                     /locus_tag="JN691_004046"
     CDS             2209..2739
                     /locus_tag="JN691_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY7245297.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQTNLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2746..2979
                     /gene="mbeD"
                     /locus_tag="JN691_004047"
     CDS             2746..2979
                     /gene="mbeD"
                     /locus_tag="JN691_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAY7245298.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSERTERLSQLYSENRR"
     gene            complement(3524..3991)
                     /locus_tag="JN691_004048"
     CDS             complement(3524..3991)
                     /locus_tag="JN691_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245299.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRALQKLQIQEVCNSTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          992 a          935 c         1216 g          964 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcttg gtcttttgct cacctgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cttggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagtcag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccctgacac ccgctaacac ctgctgacgc gccctgacgg gcttgtctgc
      901 tcccggcatc cgctcacaga caagctgtga ccgtctccgg gagctgcata tgtcagaggt
      961 tttcaccgtc atccccgaaa cgtgcgaggc agctgcggta aagctcatcg gcgtggtcgt
     1021 gaagcgattc acagatatca gcctgttcat ccgcgtccag ttcgttgagc ttctccagca
     1081 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttag
     1141 tcactgatgc ctccgtgtaa gggggatttc tgttcaggag gtaatgatac cgatgaaacg
     1201 cgagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgctg
     1261 tgagggtaaa cagctggcgg tatggatgcg gcgggtctgc ctgggtgagc cggttgcccg
     1321 ttccgggaaa ctgccgacac tggcaccgcc gttactgcgt cagctggctg ccatcgggaa
     1381 taacctgaat cagacagcga ggaaggtgaa cagcgggcag tggtcttccg gtaaccgggt
     1441 tcaggtggtg gccgcactga tggccatcgg ggacgagctg cgccggctgc gtctggccgt
     1501 cagggaacag gggacgcggg atgatagtta agtttcatgc ccggggaaaa ggtggtggca
     1561 gtggtccggt tgattacctg ctgggaaggg agcgtaaccg ggaaggggca acggtgcttc
     1621 agggtaatcc ggaagaagta cgggaactca tcgatgccac gccatttgcg aagaaataca
     1681 cgtccggtgt tctgtcattc gcggagaagg agctgccgcc gggaggacgt gaaaaagtga
     1741 tggcgagctt tgagcgtgta ctgatgcccg gtctcgaaaa aaatcagtac agcatcctgt
     1801 gggtggagca ccaggacaag ggacggcttg agctgaattt tgtcattccg aacatggagc
     1861 tacagaccgg aaaacgcctc cagccgtact acgaccgcgc agacaggcct agaattgatg
     1921 cctggcagac gctggttaat caccattacg ggctgcatga cccgaacgcc ccggagaacc
     1981 gcaggacgct gacactccct gataatctgc cggaaacgaa acaggcgatt gctgagggcg
     2041 ttacacgcgg gatagacgcg ctttaccatg ccggagagat aaaaggccgt caggatgtga
     2101 ttcaggcgct cacagaggca gggcttgaag tggtcagggt gacgcgaagc agtatcagca
     2161 ttgcagaccc gaacggcggg aagaatatca gactgaaagg agcattctat gagcaatctt
     2221 ttacagacgg gcgcggagtt cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa
     2281 atgctgaacg acgagttcag gaagctcgaa gaatctgtaa gcaaggctgt gacatcaaac
     2341 gagacgaaaa tcaaagacgc tatagccctg ttcacagctt cgacagagga atcactgaaa
     2401 aaacaccggg aaggggtgaa agaggcgatg atgcagcaca ggaaggacgt gttaaagctg
     2461 gcagggaata cgggcatgat gttactgggg atggtcattt tcctgtttac cgtgagtggc
     2521 gggacgctct ggtatcttgg cgggatgata cagacgaacc tggaagaaat caggaagcag
     2581 aacgagagtc tggagaagct gaacgcgaag acatggggcg tgcgttatca cgaggggagc
     2641 aacggacgtt ttctggtgtt gcccgagggg atgaaagccg aaacgaactg gacgatggaa
     2701 aacgggaaac agaatgccgt aaggctggta caggagtaac agagcatgac ggagctggaa
     2761 agacatttgc tgaacgcatt agagcaactg caacaggact atatgcagcg gctgaacgaa
     2821 tgggagagcg ccttcgtgga attgcagaaa atgttttcgc ttacgcaacg ggacaacgcg
     2881 atgctgaacg agcgggtcat gcagttgagt cagcaagtgc agcacttgag cgagcggaca
     2941 gaacgcttga gccagttata cagcgagaac aggagataag ggacgaacgg ctgatacagg
     3001 aatgcgaaca tgcgttatcc ctggagcgtg agcgccagct ggaaatacag gaacggacac
     3061 tggatggccc ttcgctggga tggtaaccag agacaggcta aaatgttggc cgcgcgtttc
     3121 cggtgcattt tttgggataa tggttagttg ggggcaccgg gaacgcgctt cagtcagacc
     3181 ttgctgaaaa cacaggaacg cgttaaaacg catacgggcg cgattctggt gtgtcttttg
     3241 ttgctgtgtt ttgcctctat tgccagatcg cgctcattcc cctgaacttc tgttttcccg
     3301 ctcaggtccc cgtcttcttc gcttccgaaa gcatgaaaaa aatccgtttc agctgtctct
     3361 ggctggcaat tcctgcgtga ttcacatggc tgcatagcta tgcatgtggt taaaatttac
     3421 cgggtgcgat cgcggcagtt tttcgggggg gttgttgcca tttttacctg tctgctgccg
     3481 tgatcgcacc gaacgcgttt cagcggtgcg tacaattaag atattacgtt aaattgtgag
     3541 ggaatggtcc ccatatactg actttatcat agggataggg ttggtagtgg gtcgattggg
     3601 tttcggcatt gtttccgtta atgccaattg catttttgta tttgatgcaa cagaaggcgt
     3661 gagattctcc tgggcctgga aaggataagt gtttagaatt gatggcaaaa tatcttccgt
     3721 ccgatttttg aagtagttgt tgtttggctg tctcccaaag ggtgctgtta caaacttctt
     3781 gaatttgtag tttttgcagg gctcttatta ctgcgttgtc atgttccaat tctttagggg
     3841 agtgtattat tctctctttt aataagctgt ttaccacagg gtttagttgt tgtctaaagc
     3901 acgcagataa tgccattgct acacaatttt gttcccgagg aagcatgttt ggcggggtat
     3961 gccgtgtttg atgtgatatg tctattctca tagaaggtct ctggcttata gcatcaacaa
     4021 atacggctat aaaacaaaat tttcatagag tgattcaaga tcttcttgag atcctttttt
     4081 gtggcgcgta atctgctgcc tgcaaac
//
LOCUS       DADLSA010000240         4092 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.242, whole genome shotgun sequence.
ACCESSION   DADLSA010000240 DADLSA010000000
VERSION     DADLSA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.242"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..291
                     /locus_tag="JN691_004049"
     CDS             <1..291
                     /locus_tag="JN691_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245300.1"
                     /translation="MGLANKGWIKGEPQGGGWMIKPLGRWSLIMEIDEGFAVGMSPAE
                     LSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE"
     gene            301..543
                     /locus_tag="JN691_004050"
     CDS             301..543
                     /locus_tag="JN691_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HAY7245301.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKTLSGVMKIFHRSGSPPLRRPST"
     gene            579..3946
                     /locus_tag="JN691_004051"
                     /pseudo
     CDS             579..3946
                     /locus_tag="JN691_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         1079 a         1038 c         1017 g          958 t
ORIGIN      
        1 atggggctgg caaataaagg ctggataaag ggcgaaccac agggtggagg ctggatgatc
       61 aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtatg
      121 tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
      181 gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
      241 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
      301 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
      361 ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa
      421 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaacattg
      481 tctggcgtta tgaaaatttt tcaccggagt ggcagtccgc cattacgcag gccatcaact
      541 tgatcggcga acacaaaccc tcagtcccgg cccgcacaat ggcggcacta gcctttatcg
      601 cgcaaaatga caaccaacag ttgctcgacg aaatcgtcca acaagagggg ctagaatatg
      661 cgactgaggt ggtgattgca cgccagttta ttgcacggtg ttatgagagt gatcctctgg
      721 tagtcacatt gcagtatcag aacgaggact atggctatgg ttatcgctca gaaacctata
      781 acgaattcga tctccgactg cgtaaacacc tctctctggc agaggaaagc tgctggcagc
      841 gttgcgccga caaactcatt gccgcactgc caggaataac caaagttcgc cgccctttta
      901 ttgcgctgat cctccccgaa aaaccagaaa tcgccaatga gttggtaagc cttgaatgcc
      961 cgcggactca ttttcattct aaggagtggt taaaagttga tgctactgac cccaaagcgg
     1021 tgagaaaact cgaacgctac tggagccagg atatatttag cgatcgagaa gccagctaca
     1081 tgtcgcatga aaaccacttc ggctacgcgg cctgcgctgc ccttttgcgc gaacaaggac
     1141 tggcagtcgt tccgcgcctc gcgatatatg cccataaaga agattgcggc agtctgctgg
     1201 tacaaattaa ccatccgcaa gtcatccgca ccttgctgtt agttgctgat aaaaacaaac
     1261 ccagcctgca acgtgtggct aaatacagta aaaacttccc ccatgcgaca ctcgccgcac
     1321 tggcagaact gctggcgtta aaagaaccac cagcccgccc aggttatcca atcatcgaag
     1381 acaaaaagct gcctgcacag caaaaagctc gagatgaata ctggcgtacg ctgttacaaa
     1441 cgctgatggc atcgcagcca caactggcag cagaagtgat gccgtggtta agtactcaag
     1501 cccgggcagt gctgaatagt tatttatcgg caccgcccaa accagttatt gatagcaccg
     1561 ataacagcca aatgcctgaa atcctcgttt caccaccatg gcgtgggaga aagaaaaaga
     1621 cttccatacg tcacttttcg ctgcctgatc ttacgcttgc accgcaaacg tacaggtcgc
     1681 cgaataaaca ctggacgccg gaacagcaaa ctgcaattga ccattttagc gccatgtctt
     1741 ttgacgaacg tcttgctacg agaggagcgg atatcttctt gcgggaatta ggttttgacg
     1801 ttcatagcct ggaatttaat aagcgtcttc ttaatggaca gttggatgcc tttgatgctc
     1861 agcaactgca agaatacaca tcaataggac tattgcgtac ttatcacgcg catcaggcca
     1921 aatctaaaca gtaccgggaa gacgccacca atgcattact ggcacaagat agcattgcgt
     1981 tgatgggagg gtggcgaact tttcagcacg gattttacta tggtaatgaa cagcgttggg
     2041 ggatttggaa tctgtacctt atcgcgcaga tgccgcgcga aatggcggta tcgtgctggc
     2101 aacaaattgt cgaggctgat tttcattaca ccggggtgga atatttgtta agtgtgctgg
     2161 gtaccgatgc tttgcctgga ttaaacaccg cctttgcccg acatccaaaa gagatatttc
     2221 cgctgctgat tcatttcggc gcaacagaac tggccctgcc cattgcccgc gtctggcacc
     2281 tttttgcggg ccagcgtaat ctggctcgcc agtggatttt acaatggccg gaacatacgg
     2341 ctactgcact tatccctctt gtcttcacca aatctagcga taaaagcgaa gctgcattac
     2401 ttgccctgcg tttactttac gaacatgggc atggcgaatt gctacaaact gtagccaatc
     2461 gttggcagcg tacagatcta tggcctgccc tggaacaatt acttaaacag agtccaatag
     2521 agatttaccc ggcacgcatt ccaaaagccc ctgatttctg gcatccgcaa atgtggtcca
     2581 ggccgcgcct tatcactaat aatcaacctg ttaccgatga cgctctggaa attatcggcg
     2641 aaatgctgcg ctttacccag gggggacgtt tttatagcgg gctggaacaa ctgaaaacgt
     2701 tctgccagcc acaaacgctg gcagcttttg cctgggatct cttcactgcg tggcaacaag
     2761 ctggtgcccc cgcaaaagac aactgggcat ttctggcgtt aagtctcttt ggtgacgaaa
     2821 gcacggcacg ggatctaacg acacatatcc tcgcctgccc acaagaaggc aaatctgccc
     2881 gtgcggtcag tggcctgaac atccttaccc tgatgaataa tgatatggcg ctgatgcagc
     2941 tgcatcatat atcgcaacgg gcgaaatccc gccccttacg tgataacgcg gcggaatttc
     3001 ttcaggtagt cgcagaaaat cgcgggctaa gccaggaaga gttagcggac agattagtcc
     3061 caacactggg ccttaatgat ccgcaggcgt tgagttttga ttttggtccc cggcagttta
     3121 ccgttcgctt cgatgaaaac ctcaacccgg ttatctttga tcagcaaaac gttcgccaga
     3181 aaagtgttcc ccggttgcgc gccgatgacg atcaactgaa agcgcccgag gcactggccc
     3241 gactaaaagg gttaaaaaaa gatgctactc aggtgagcaa aaacctgctc ccgcgtcttg
     3301 aagctgccct acgtaccacc cgacgctggt cgctggcaga ttttcattct ctgtttgtta
     3361 atcatccctt tacccgtctg gttacccagc gattaatatg gggggttatc cggcaaatga
     3421 accgcgtcgt ttactcaacg cctttcgtgt ggccgcagag ggggagttct gcaatgcgca
     3481 agatgagcca attgacctgc ctgcggacgc tctgattggc attgcccacc cgttagaaat
     3541 gacagcagaa atgcgcagtg aatttgcaca gctttttgcc gattacgaaa ttatgccgcc
     3601 ttttcgccag ttgtcgcgcc gcacggtgct gctcacacct gacgagtcaa ccagtaacag
     3661 cctgactcgc tgggaaggta aatccgctac cgttgggcaa cttatgggaa tgcgatacaa
     3721 aggctgggag tcaggttatg aggacgcatt tgtctatgac ctgggtgagt accggctggt
     3781 ccttaagttt tcacccggtt ttaaccacta caatgttgat agcaaagcgc taatgagctt
     3841 ccgttctctt cgagtgtacc gtgacaataa atccgtcact tttgccgaac ttgatgtgtt
     3901 tgatttgagt gaggcgttaa gcgcacctga cgtcattttc cattaaaaca aagcgggcag
     3961 tgatgcgact gcccgttatc tacacgactt accagcgggg aaagcgatga tcgtgcaaaa
     4021 agagctggtt gctatttacg attatgaggt ccctgtacct gaagatccgt tttccttcag
     4081 acttgagatc ca
//
LOCUS       DADLSA010000241         4022 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.243, whole genome shotgun sequence.
ACCESSION   DADLSA010000241 DADLSA010000000
VERSION     DADLSA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.243"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..305)
                     /locus_tag="JN691_004052"
     CDS             complement(<1..305)
                     /locus_tag="JN691_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7245302.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMELSINKNWIDKEEYPQSAAIDLRCV"
     gene            complement(516..1913)
                     /gene="ccp"
                     /locus_tag="JN691_004053"
     CDS             complement(516..1913)
                     /gene="ccp"
                     /locus_tag="JN691_004053"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAY7245303.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2318..3967
                     /gene="treF"
                     /locus_tag="JN691_004054"
     CDS             2318..3967
                     /gene="treF"
                     /locus_tag="JN691_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAY7245304.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGKP"
BASE COUNT         1041 a         1016 c          936 g         1029 t
ORIGIN      
        1 tttacgcaac gcaggtcgat ggctgcggat tgcggatatt cttctttgtc gatccagttt
       61 ttattgatcg acaactccat caatttatgg acgttttcgt cgtcccaggt ctggcagaaa
      121 gtggctaggt tctgacgagc gttgccatca agatataatt catcattgat aatctggaat
      181 gcgacatcat cgcgcatttc gtgcagcgga aatcgttttg actccgcgat agtagaaatg
      241 gcctttgcgc caaaacgtga atcgagtagt tctgagcgga aatccgttaa cagcttctgg
      301 tccatttcga actccttaaa tttatttgaa ggcaataaaa aagtaggatt tatccgcaat
      361 ggaagcaagg cattacaggc ttaatttaaa taacaaaatc ctaagcagaa aaacgacttt
      421 ttataatata aacatttaac atgataatat taaaaacaga ctttattatc gtaataaaaa
      481 tcgcccaaac attcctgttc gggcgatcaa attaattatt gtttatcctg catatacggc
      541 gtgtacaccc cgttcagact gtgcaggaaa gctacgatat catccacatc ctcctgcggc
      601 agctctttgc ctacctgata gcgcagcatc agtttcaccg ccccgtccag cgtcggcacg
      661 tcaccgcgat ggaagtacgg tgccgttaaa gcaacgttac gtaaaccggg tactttctga
      721 cgcaatttat cacgctcttc tttagtcaca ttcatacgac caatatccgc cgccgtaatt
      781 tccccaaagt taaagtcttt tttcagcccc aacggttcaa aggaacgtcc gccgagaata
      841 ataccaccat gacaagttgc acatttatta tctttaaata attgatagcc ttttttctgt
      901 tgcgccgtca gcgcattttc atctccacgc aaccatttat caaatgggga atccggcgta
      961 attaatgttt tctcaaattc agcaatggca tcagtaatat tttcgccact gaaaccttgc
     1021 ggataaactt cgaggaactg cgctttaagc tgcggatctt tttccagctt agcaataatt
     1081 tcgtcccagg atttcgacgc catttcaatc gggttcaacg gcggtccacc tgcctgatcc
     1141 tgcaatgttg ccgcacgacc atcccagaac tgctcaacgt taaatactga gttaaatacc
     1201 gtcggcgcat taatcggccc aactgcgcca ccaacaccaa tcgatgtttt tctgccatcg
     1261 acgccccccg cattcaacgc atggcaatga gcgcatgaaa tggtgctatc agccgataaa
     1321 cggggatcgt gatacagcgc aaagcccaac gccacttttt gcgcatcggt aggcagtttt
     1381 tgcgggatgg gctgcaccgg ttcattgcga tgctccggag cagtatcatt gctggcgtaa
     1441 tattccgcgc gctgttttgc aatccaggcc agtatttccg cccgctcttc atcactcacc
     1501 ttacccgccc agtgtagcgc ggtataacgc gttggtggca tagtttcata ctgcatcacc
     1561 cattcaatct tattcagatc gctttgcgaa acgggtttat cagccagcag tgccgcacgc
     1621 acggcctcga ggttaaaaga tttatatcca agcttaatgt cgtaatccat caactgtttc
     1681 gcgccaggaa tataataata ggcgggtaat tctgccgaag gcgtgtggca atagtcgcat
     1741 cctttttcgc ggagaaagcc taaaacctta ttattttcac tgacagcaga tgcctgaaca
     1801 tcggcctgtt tactgcgttt attatcgtgg taccacacat aaccagataa ccctaaatag
     1861 caaatcgcga cgccagccag gccgatcgcg gtaatacgtg agaccatttt cattattctt
     1921 ccctcacggt ggtgagttat tgtgacccag aaaattttcc agaatgctgc aaacgtctct
     1981 gcgagacaaa gccatcataa aaaacaggga gatacgcttt ttgataggaa tcaatcaatt
     2041 acatgaatat aaactatcaa taagatagcc tgtgcctatt acccagatca ggaatggcat
     2101 atcatgattg cggcgagagc aggattgaat gttctgaaaa tgaaaaacaa caccaaatcc
     2161 gtatttttat aaattccatt gatattagtg cgttatattt tttgacaagt taataaacgt
     2221 gatccaccgc acgctttgtc gcccaccagg cggagcgaat gactaccctt aaagaaaagc
     2281 ccgataatta gcgacgaatt tcggaggttg gatccttatg ctcaatcaga aaattcaaaa
     2341 ccctaatcca gacgaactga tgatcgaggt cgatctctgc tatgaactgg acccgtatga
     2401 attaaaactg gatgagatga tcgaggcaga accggaaccc gagatgattg aagggctgcc
     2461 cgcctctgat gcgctgacgc ctgccgatcg ctatctcgaa ctgttcgagc atgttcagtc
     2521 ggcgaaaatt ttccccgaca gtaaaacctt tcccgactgc gcacccaaaa tggacccgct
     2581 ggatatttta atccgctacc gtaaagtgcg ccgtcatcgt gattttgact tgcgcaagtt
     2641 tgttgaaaat cacttctggc tgccggaggt ctactccagc gagtatgtat cggacccgca
     2701 aaattccctg aaagagcata tcgaccagct gtggccggtg ctaacccgcg aaccacagga
     2761 tcacattccg tggtcttctc tgctggcgct accgcagtca tatattgtcc cgggcggccg
     2821 ttttagcgaa acctactatt gggattccta tttcaccatg ctgggactgg cggaaagtgg
     2881 tcgggaagat ttgctgaaat gcatggccga taacttcgcc tggatgatcg aaaactacgg
     2941 tcacatcccc aacggcaacc gcacctatta tttgagccgc tcgcaaccac cggtttttgc
     3001 gctgatggtg gagttgtttg aagaagatgg tgtacgcggt gcgcgccgct atctcgacca
     3061 ccttaaaatg gaatatgcct tctggatgga cggtgcagaa tcgttgatcc ctaatcaggc
     3121 ctatcgccat gttgtgcgga tgccggacgg atcgctgctc aaccgttact gggacgatcg
     3181 cgacacgcca cgtgacgaat cctggcttga ggacgttgaa accgcgaaac attctggtcg
     3241 cccgcccaac gaggtgtacc gcgatttacg cgcgggagcg gcctccggtt gggattactc
     3301 ttcccgttgg ctgcgtgata ctggtcgtct ggcgagcatt cgtaccaccc agttcatccc
     3361 catcgatctg aatgccttcc tgtttaaact ggagagcgcc atcgccaaca tctcggcgct
     3421 gaaaggcgag aaagagacag aagcgctgtt ccgccagaag gccagtgccc gtcgcgatgc
     3481 ggtaaaccgt tacctctggg atgatgaaaa cggcatctac cgcgattacg actggcgacg
     3541 cgaacaactg gcgctgtttt ccgctgccgc cattgtgccg ctctatgtcg gcatggcgaa
     3601 ccatgaacag gccgatcgtc tggcaaacgc cgtacgcagc cggttactga cacctggcgg
     3661 gattttggca agcgagtacg aaaccggtga acagtgggat aaacccaacg gctgggcacc
     3721 gttacaatgg atggcaattc agggatttaa aatgtatggc gatgaccttc tgggtgatga
     3781 aatcgcgcgc agctggctga aaacggtgaa tcagttctat ctggaacagc acaaaatgat
     3841 cgagaaatac catattgccg atggtgttcc ccgcgaaggc ggcggtggcg agtatccgtt
     3901 gcaggatggg tttggctgga ctaacggtgt ggtacgccgt ttaattggtt tgtacggcaa
     3961 accataatat ttttacagcc agccgctaac ttcctgctgg ctgtaaaatt atcctcttca
     4021 gg
//
LOCUS       DADLSA010000242         3998 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.244, whole genome shotgun sequence.
ACCESSION   DADLSA010000242 DADLSA010000000
VERSION     DADLSA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.244"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            292..888
                     /gene="wrbA"
                     /locus_tag="JN691_004055"
     CDS             292..888
                     /gene="wrbA"
                     /locus_tag="JN691_004055"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY7245305.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            909..1136
                     /locus_tag="JN691_004056"
     CDS             909..1136
                     /locus_tag="JN691_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245306.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(1174..2415)
                     /gene="agp"
                     /locus_tag="JN691_004057"
                     /pseudo
     CDS             complement(1174..2415)
                     /gene="agp"
                     /locus_tag="JN691_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
     gene            complement(2708..3964)
                     /locus_tag="JN691_004058"
     CDS             complement(2708..3964)
                     /locus_tag="JN691_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HAY7245307.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
BASE COUNT         1111 a          924 c          840 g         1123 t
ORIGIN      
        1 ggactaagct tagtgaactg acgcgggcag acccgcgttt ggtaatatcc tgcaacagct
       61 tcttagcgag aatagatatc atctccaata attatccata agccgctctt atgattaaaa
      121 aaacgcgatg agataaaaat gtgtcatttt gcgacaaaat tacgtgcttg tgaaagttgt
      181 tataaatcaa ataagtggtt gtgaaatttg cactctgaaa aggacgtctt atctttaaat
      241 aagtggtagc gaatcgctac ggaatagaga taacacgagg agtggttaga aatggctaaa
      301 gttctggtgc tttattattc catgtacgga catattgaaa cgatggcacg cgcagtcgct
      361 gagggtgcaa gcaaagtgga tggcgcagaa gttgtcgtta agcgtgtacc ggaaaccatg
      421 ccgccgcaat tatttgaaaa agcaggcggt aaaacgcaaa ctgcaccggt tgcaaccccg
      481 caagaactgg ccgattacga cgccattatt tttggtacac ctacccgctt tggcaacatg
      541 tccggtcaaa tgcgtacctt cctcgaccag acgggcggcc tgtgggcttc cggcgcacta
      601 tacggaaaac tggcgagcgt ctttagttcc accggtactg gcggcggtca ggaacaaacc
      661 atcacatcca cctggacgac ccttgcgcat cacggcatgg tgattgtccc cattggctac
      721 gcagcgcagg aattatttga cgtttcacag gttcgcggcg gtacgccgta cggcgcaacc
      781 accatcgcag gcggtgacgg ctcacgccag ccaagccagg aagaactgtc tattgctcgt
      841 tatcaagggg aatatgtcgc aggtctggca gttaaactta acggctaatc ttcaacagga
      901 ggatacgcat gccaactcaa gaagcgaaag ctcatcacgt cggtgaatgg gcatctctgc
      961 gcaatacgtc gccggaaata gccgaggcca ttttcgaagt cgccgggtac gacgaaaaaa
     1021 tggcagaaaa gatttgggaa gaaggtagtg atgaagtgtt agtcaaagcc tttgctaaga
     1081 ccgataaaga ttcgcttttt tggggcgaac agaccatcga acgtaaaaac gtttaaccag
     1141 cgactccccc gcttctcgcg ggggagtttt ctgttatttc accgcctcat tcaacacgct
     1201 atcaaactta tccatcgggc agaaaccgtt ggcatctatc gggcaaccgc ttaattccag
     1261 cgtcacgcgc tgcgcaggtg cctgcagggt taacgcatcg gcattacgta actgttccgc
     1321 actctggtac acatattcaa ttttcatcaa atcgcgattg gctttgctgt catgccaacg
     1381 ctggaaaacg attttgccgc caatcggcgt gcgttcgttc tggtcatgca actgatacgg
     1441 tttgaaatcc agcgccgtta acagagaggc tatgttggag tcgtgcccaa ctaacaccgt
     1501 gattttcgga gcactggcgc gatcggtgac cagggctttg tcgatataac tgaccagcgg
     1561 tttcgcaaca ttgcgcgcca cttccggtga ggtaaacagg ctgtcctagt aaccgttttt
     1621 cagcttcgac aataccttcc actgctggtc agatttgatt tctccctagg ccacctgatc
     1681 catcggaaaa ccttcgtagt attgcaaagt aaacgcatct accagcgagt tgccgacttt
     1741 cagcggcccg gaaacacctg gttcttgttg atacttggcg ctaaaggtat ttttgccatc
     1801 caccagcgaa cactgttgtt tctctttaca ggcaggggaa tctttatagt taacgatttt
     1861 ttccagtagc tggtagctgt cggtaagctg gagtttgctg agctctttct ccattgccgc
     1921 caccgcctgt tcactgaatg cggcggaatc atcggtgatc atcgggttaa aggttgggtc
     1981 catagtgccc attttttcct ggtgatgcac aggaatatca cacccaggga atgcaccagt
     2041 aataaagaac tgggcggtag cgacggtacg ttgcagacta ttggcatagg cataaacggt
     2101 gtccggcggc gggcattccc ccgatttcac tatcccctgc tctgccagcc attcacgcat
     2161 gtaatggccc atatacactt cgagcacgcc acctttggtg gtgagttgcc caccggggac
     2221 gtcccattcg ggccatttat tcggcgtcga ctgctccagc acactgccat tgttcgccag
     2281 cggcgcacgt aagttatggc ggctcatcat gagcacttgc tgtagctgat agccttccgg
     2341 tacggtttgt gcctgagcat ttgaagcgag taaaactatc cctgccacag ttgcggcgat
     2401 tagcgttttg ttcattcctg gcacctcttt tgttatcaat cattaaagag tgtgacagaa
     2461 atatgacttt tttccgggaa caggttcgca aaatggcttg ttgaacgcct gtttttctat
     2521 atacatcttc aaaaacaggc aaggtttgac gcaaactatg cctgattagc ccctgcctcg
     2581 cgtacgccgc tgataattgc aactgtggct aaacagaata tctgcggcgt taccaataca
     2641 gagacaatat ttaccaataa aaaagtgtga gactaataac aatggttttt agcatatgcc
     2701 tttgccatta agttgcataa cgatttaaat agtcaagttg cgcattcatt tccttatttt
     2761 ttaacttata agcctgagaa aaaagttcag gagggaggta aacatcataa tgattgccag
     2821 tgtttattaa ataaccatac tcaagatctt catctgttga aaaggattca tttcccaagg
     2881 agattgatgc cacattacta ttaacaataa ataatgagca aatgctatct gaaagaacag
     2941 caagaaaatt cttcaccgct ttaatcctat ccgacgtgtc ggtatctaaa gcaagcaaat
     3001 tcttgttatc attaaatata aactcgggta aatgcagtat gcattttttg tcattcaagc
     3061 aactatcaat gaattgtttt ttaaaaaaca gaaagctcca tgacataaaa ctacccacac
     3121 cctgaatttt gttgtcagat tgcagatcaa cgccaaataa tgtctttaca tcttccctga
     3181 gattatagaa tgccgtatca acaagagcct cgatcgtttg tttatcaaca attttaataa
     3241 actcttcgta tacttgcttt tgcgtcttat ggctgcccag cgcccatgaa tactcatgtt
     3301 cttttaaaac cagtatggct cttaacagac aatatccatc gccatctact ggagcgtatt
     3361 ttaatccata tgttttatta atttcactct gaataaaaga tatgccagac aaatcaatac
     3421 cagtatctaa atcagatatt cccggcatat ttatctgttc tggctggata ctcatatttt
     3481 catcatctaa acaatactca gaaaaaacat catcacacgc attaatgttt tcaatatatt
     3541 ccataaagcc ataccaataa taacctttaa aatctgtgat agactctgaa cctctgcccg
     3601 gcaaggacac tctaacaata tcaacatctt gaacatattg tatagtatat gtaatacctc
     3661 ctgaaacccc tgaataaatt tgtgtatcat tagtacgcaa tgttctcata aataatgata
     3721 gatcattatc attctgcatt tcctcaaatt ctttttctag tgattcacat tccagacatg
     3781 gagtatcctc ttcatttact ttacgtctga gttcattgta caaccgcata aaacacacca
     3841 gtgtgttttc aaaaagcgtg attgaatatg tttgattttt ttcgtactca ttttttatat
     3901 cgttccaggc ctgtttcaaa taattatttg cagtgcaact aattccatgt atattactac
     3961 ccatatatag cgtctataaa atttaataaa taatgacg
//
LOCUS       DADLSA010000243         3918 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.246, whole genome shotgun sequence.
ACCESSION   DADLSA010000243 DADLSA010000000
VERSION     DADLSA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.246"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            156..908
                     /locus_tag="JN691_004059"
     CDS             156..908
                     /locus_tag="JN691_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY7245308.1"
                     /translation="MPVSLSAQLGSKEQADVRLAGSVMSALRVSMPGIVQSFDPDTVT
                     VVVQPAIKGYEPDSNGINQSTTLPLLVDVPVVFPRGGGCTLTFPVKAGDECLVVFADR
                     CIDFWWQSGGIQEPVDDRMHDLSDAFCIVGPQSQARKISGINTSATQLRSDDGSTYFE
                     LNPDTRKIKIVAPGGLDVVAPLADFSEKVTIHGLLTWMGGMVGSVVSGVASKITGAVE
                     FLGSVKANGKPIDDTHTHGGVQRGGSNTDGVN"
     gene            908..1261
                     /locus_tag="JN691_004060"
     CDS             908..1261
                     /locus_tag="JN691_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245309.1"
                     /translation="MRYRREDADGDYTFGSGDDTWLINSPEAVAQAVKTRFELWYGQW
                     FLDTTEGTPWIQSVLGKQKPETYNLAIRKRILETRGVKSILSFNTTVDTTTRRVRFST
                     EIDTLYGITTVTSEA"
     gene            1261..2460
                     /locus_tag="JN691_004061"
     CDS             1261..2460
                     /locus_tag="JN691_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245310.1"
                     /translation="MALNLDSLGLSAKVTAEGISAPDYQTILSTLISYFQQIYGSDAY
                     LEPDSKDGQMVALMALAIHDANNMAITVYNCFSPATGYGAALTSNVKINGISRKGATN
                     STVDLLLTGTAGTTIINGSVKDGNNVIWRLPASVVVGVDGTVMATARCSVSGAVAALA
                     GTITEINTPTRGWVSVINPAAATVGSPAETDAELRIRQSQSVALPSITPFEALDGAVS
                     NVAGVTRHKLYENDTGSEDGNGLPPHSVAVIVDGGDVTDIAQAIRGNKGQGTATHGTT
                     SVTVPDKYGNPHVIKFSRSSDVPVYARIKLKVFTGYTSQIGQQIQQVISDYINSLTIG
                     DSVLLSRIYSPANLGVVSGWNARYYDIQELTIGKSPGALSSSNIDIRYNESASCTPEN
                     IVITVES"
     gene            2457..3137
                     /locus_tag="JN691_004062"
     CDS             2457..3137
                     /locus_tag="JN691_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HAY7245311.1"
                     /translation="MSKYTELITNYHATKPKFLAHVDLMTRPLIDVAAATRGLITAFD
                     IDSAVGVQLDILGLWIGRSRVVSQPISGVYFSWDTDGLGYDQGVWQGPYDPDSGYMYL
                     SDETYRVILKAKIAINNWDGRNDSLPAILDAATAGSGLRMQIVDNQDMTISVWVFPDT
                     DISDVSCELIAAIKQGYLTVKAAGVWAGGIETPSVETPSEGSKFFGFDMDNEFISGFD
                     VGAWGILL"
     gene            3464..3918
                     /locus_tag="JN691_004063"
                     /pseudo
     CDS             3464..3918
                     /locus_tag="JN691_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023101.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          991 a          827 c         1063 g         1037 t
ORIGIN      
        1 taaaaaaaga agaatgtaga ttggtgctat ctgaaaataa gaaatgagtt cgctaaatta
       61 acttttcacg gtaattgctt ctatcaccat ggaaagaaaa ctagctacat aaaacccgcc
      121 ttcgagtggg ttttttgctt tctggaggca tatgaatgcc agtttcttta agcgctcaac
      181 ttggtagcaa agaacaggcc gatgtaagac ttgctggctc tgtcatgtcg gcgctgcgtg
      241 tttctatgcc cggcatcgtc cagtcatttg atccggacac ggtaacggta gttgttcaac
      301 cagcgattaa aggctatgag ccagactcaa atggaatcaa tcagtcgacg acattaccct
      361 tgctggtgga tgtgccagtg gtattcccgc gcggcggagg ctgtacgttg acttttccgg
      421 taaaagccgg ggatgagtgt cttgtcgttt ttgccgatcg ttgtattgat ttctggtggc
      481 agagtggcgg gatacaggag ccggttgatg acagaatgca tgatttatcg gatgcgtttt
      541 gtattgtagg tccccagtcg caggcgagga agattagcgg tattaatacc agtgccacac
      601 agttgcgtag tgacgacggc agtacctatt ttgagcttaa tcctgatacc aggaaaatta
      661 aaattgtcgc tccgggtggt cttgatgtgg ttgcccctct ggctgatttt tctgagaaag
      721 taaccattca tggcctgtta acctggatgg gtggcatggt ggggtctgtt gtttctggtg
      781 tggcttcaaa aatcactggt gctgttgagt ttttgggtag cgtgaaggct aacggcaagc
      841 caatcgatga tacgcacact catggtggtg ttcagcgcgg tggaagcaat accgacggag
      901 taaactgatg cgatacagac gtgaagacgc cgatggcgat tacacctttg gcagcggtga
      961 tgacacctgg ctgattaact caccggaggc cgtggcgcag gcggtaaaaa cgcgattcga
     1021 attgtggtat gggcaatggt ttctcgacac caccgagggg actccgtgga tccagtccgt
     1081 actcggtaag cagaagccgg aaacctacaa cctggcgatc cgtaagcgca tcctcgaaac
     1141 gcggggcgtt aaatcaatcc tctctttcaa tactacggtg gataccacga cccgacgtgt
     1201 caggttttcc actgaaatcg acactctcta tggaataacg actgttacat cggaggcgta
     1261 atggctctga accttgattc tctcggttta tctgcaaagg taaccgcgga ggggatcagt
     1321 gcgcctgatt atcagacgat actcagcacc ctgattagct attttcagca gatttatggt
     1381 agtgatgcct acctcgaacc ggacagtaaa gacggtcaga tggtggctct gatggcgctg
     1441 gcgattcatg atgccaataa tatggctata actgtctaca actgtttttc accggcaacc
     1501 ggctatgggg ctgcactgac cagtaacgtg aaaataaatg gtatttcacg taaaggcgcg
     1561 acgaactcta cggttgattt gcttcttaca ggaacagccg gaacaaccat cattaatggc
     1621 agcgtgaaag atggtaataa tgtgatatgg cgtttgcctg cgtcagtggt ggtcggtgtg
     1681 gatggtacag tgatggcgac cgcaagatgt tccgtcagtg gtgcagtggc ggcgctggct
     1741 ggaactatca ctgaaattaa tacgccaacc cgtggctggg tttcggtaat taatcctgct
     1801 gcggctactg ttggctctcc ggcagaaact gatgctgagt tacgtatccg ccagtcgcaa
     1861 agtgttgcgt tgccatcaat aaccccattt gaagcactgg atggtgctgt ttctaatgtt
     1921 gccggtgtaa cccgccacaa actctatgaa aacgatactg gttcggagga cggtaacggg
     1981 ttaccgccac actctgttgc tgtaattgtg gatggcggtg atgtgacgga tattgctcag
     2041 gctatcagag ggaataaagg ccaggggaca gccactcacg gtacaacatc cgttacggtt
     2101 ccggataaat acggcaatcc ccatgtaatc aaattctcgc gttccagtga tgtgcctgtt
     2161 tatgcccgga ttaaattaaa agtttttacg ggttatacct cacagatagg gcagcagatc
     2221 cagcaggtta tttccgacta tatcaatagt ctgacgattg gtgattcggt ccttttaagt
     2281 cgcatttact caccggcgaa tcttggcgtg gtgagtggct ggaatgcacg ctattacgat
     2341 attcaggagc tgacgattgg gaaatccccg ggggctttgt cgtcatcaaa cattgatatc
     2401 agatacaacg aatctgcgtc ctgtaccccg gaaaatatcg ttataacggt ggagtcatga
     2461 gcaaatacac cgaactaatc acgaactacc acgccaccaa acctaaattt cttgcgcatg
     2521 ttgatctgat gacccggcca cttattgatg ttgcggctgc caccagaggg ctgattactg
     2581 catttgatat tgactctgcg gttggtgtgc aacttgacat tctgggattg tggatcggac
     2641 gtagccgtgt tgtcagccag cctatctcag gtgtctattt cagctgggat accgacgggc
     2701 ttggatatga tcagggggta tggcagggac catacgatcc tgattccgga tacatgtacc
     2761 tcagcgatga aacttatcgt gtcattctta aagcgaagat tgcgattaat aactgggatg
     2821 gacggaatga ttcgcttccg gcaattcttg acgctgcaac agcaggatcc gggctgcgaa
     2881 tgcagatagt cgataaccag gacatgacga tatcggtctg ggtctttcct gatactgata
     2941 tttcagatgt atcgtgtgag ttaatagcgg caattaaaca ggggtatctc acagtaaaag
     3001 ccgccggggt atgggcgggt ggcattgaaa caccttcggt ggaaacccca tcggaaggct
     3061 caaaattttt tggttttgat atggataacg aattcatcag tggttttgat gtaggagcat
     3121 ggggaatatt actctgatgg cgaaaaatga ctttaaagcg tttgcaacgg gtaaaaatgc
     3181 caatgttatg tcgcaggagg aatgggaagc gttgcctgcg cttttatccg gatttacagc
     3241 agggaaagca tccagtgcgc aagtcaataa ggttattcgg caggccagct ttattgctgc
     3301 agctctggcc cagtttgtaa gtgacaaaac gcaacgggat gtgcttgata atggtgatct
     3361 gcccggtttt gttgaattgc tgggatcggg gtttgctgtt gaatacctga gccgcaagaa
     3421 tccgtttggc gatatcaaat cggatggcac ggtgaaaacg gctctcgaaa accttggttt
     3481 acaggaaacg gtaaacaagg ctggtaacgc tgtccaaaag acaggcgata ccttgtccgg
     3541 tggacttact tttgaaaacg actcaatcct cgcctggatt cggaatactg actgggcaaa
     3601 gattggtttt aaaaatgatg ccgacagcga tactgattca tacatgtggt ttgaaacagg
     3661 tgacaacggc aatgagtatt tcaaatggag acaccgcctc gctggcggcc agctcaaaga
     3721 gctgatgaat cttaaatggg attcactaaa tattctggtt aatgccgtca ttaatggttg
     3781 ccttggcatt ggtacgacga atgcgttagg tggaaattca attgctttcg gggataacga
     3841 taccggcctg aaacagaacg gcgatggtct gctggatgtt tatgcgaatg gacagcatgt
     3901 atttcgtttt cagaatgg
//
LOCUS       DADLSA010000244         3865 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.247, whole genome shotgun sequence.
ACCESSION   DADLSA010000244 DADLSA010000000
VERSION     DADLSA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.247"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..512
                     /locus_tag="JN691_004064"
     CDS             <1..512
                     /locus_tag="JN691_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheinyl transferase"
                     /protein_id="HAY7245312.1"
                     /translation="SISYAGNMVGVALTTEGECGLDMELQRATRGFHSPHAPDNHTFS
                     SNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLNDDPRDLQLLPIAGRLKCAHVNH
                     VEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWKSLPDIHSRANNPTSRMMRFAQL
                     STVKSFSPN"
     gene            534..1100
                     /gene="yieF"
                     /locus_tag="JN691_004065"
     CDS             534..1100
                     /gene="yieF"
                     /locus_tag="JN691_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HAY7245313.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMDVIGGACCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1154..2491)
                     /locus_tag="JN691_004066"
     CDS             complement(1154..2491)
                     /locus_tag="JN691_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7245314.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            2656..3321
                     /gene="yieH"
                     /locus_tag="JN691_004067"
     CDS             2656..3321
                     /gene="yieH"
                     /locus_tag="JN691_004067"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HAY7245315.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            3388..3861
                     /locus_tag="JN691_004068"
     CDS             3388..3861
                     /locus_tag="JN691_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HAY7245316.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1029 a          966 c          973 g          897 t
ORIGIN      
        1 tttctatttc ctatgccggg aatatggttg gcgtggcgtt aacaaccgaa ggtgaatgtg
       61 gcctcgatat ggaactacag cgtgcgacgc gcgggtttca tagcccacac gcgcccgata
      121 accacacctt ttccagcaat gaatcgctat ggatcagtaa acaaaacgat cctaacgaag
      181 cgcgggcgca gctcatcacg ctgcgccgaa gcgtgctaaa actaaccggt gatgttttga
      241 atgacgatcc gcgcgatctg cagctgctgc ccattgccgg acgcctgaaa tgtgctcatg
      301 taaatcatgt agaagcgtta tgcgacgcgg aggacgtgct ggtgtggtcc gtggcggtca
      361 cgcccgcgat tgaaaagctc agtgtctggg agttagatgg caaacacagc tggaaaagcc
      421 tgccggatat tcacagccgc gccaacaatc ctaccagccg gatgatgcgt tttgcccagc
      481 tctctaccgt gaagtctttt tcgccaaatt gatagacaac cacaggagtc attatgtctg
      541 aaaaattgca ggtggttacg ttactgggga gcctgcgcaa aggctcattt aatggcatgg
      601 ttgcacgtac cctgccgaaa attgctccgg cgagcatgga agtcaatgcg ttaccttcca
      661 ttgccgacat tcccctgtat gacgctgacg tacagcagga agaaggtttt ccagcaacgg
      721 ttgaagctct ggcggaacag atccgtcagg ctgacggtgt ggtgatcgtc acgccggaat
      781 ataactactc ggtaccgggt gggctgaaaa atgccatcga ctggctttcc cgcctgccgg
      841 atcaaccgct ggccggtaaa ccggtgttga ttcagaccag ctcaatggac gtgattggcg
      901 gcgcgtgctg tcagtatcac ctgcgccaga ttctggtttt cctcgatgca atggtgatga
      961 acaagccgga atttatgggc ggcgtgattc agaacaaagt tgatccgcaa accggagaag
     1021 tgattgatca gggtacgctg gaccacctga ccgggcaatt gaccgcattt ggtgagttta
     1081 ttcagcgagt taagatctaa ataaaaaacc cgccagtaat catgcatggc gggtttttaa
     1141 tgcgctatcg attttaatga gcgtcgataa atacaatctt caggataaac agcagcgcaa
     1201 cgatgattac gcacgggcta aggtcacgca gacgcccggt accaatcttc atcacgcagt
     1261 aggagataaa gcccagcgca ataccttcgg taatcgaaaa gctgaacggc atcatcacgg
     1321 cggtaataaa cgccggaaca gattcagtaa gatcctgcca gttcacgcga gcaagacttg
     1381 aggtcatcaa cacgccaacg tagatcaacg cgccagctgc agcgtagcct ggcaccattc
     1441 ccgccagcgg cgacagaaag ataaccagca ggaacagcag accaacaacc actgccgtca
     1501 gaccggtacg accgccaacc gatacgccgg aagaggactc aatataagcc gtaacggaag
     1561 aagtaccgat aaacgaaccg gtcacggaag agatactgtc gacatacagc gcctgcttca
     1621 tgcgcgggaa tttccccttc tcatccgcca gacctgcttt atcggtcacg ccaatcagcg
     1681 taccggagga gtcaaacaag ttgaccaaca tgaaagagaa aatcacccct gccagcccga
     1741 ggttaaacga cccggctaaa tctacatgac ccacaactgt cattacgctc ggcggtgcag
     1801 aaacgatgcc attgtagtgc acatcaccca gcatccagcc cagcagcgtc gtcaccacga
     1861 tagaaaccag caccgctgcg tgaatgttgc gcgaggccag aatagcaatg atgaaaaagc
     1921 cgaggatacc cagaagtacg ctgtgagaag tcagattacc gatgctcacc agcgtttccg
     1981 agttagcgac aatcacacct gcgtttttca gccccatcat gccgataaac aggccgatac
     2041 cgctggtaat acccacacgc agacttaccg gaatgttagc aatcatccag taacgaacgc
     2101 ggaaaatggt caaaagaagc agtccgacag cgccccagaa gattgcgccc ataccgatct
     2161 gccacggcag ccccattgcc tggacaacca caaaagcgaa aaaggcgtta agccccatcg
     2221 caggagccag cgccaccgga aggtttgcaa acaatcccat cataatgcta ccgaaagcag
     2281 caatcagaca ggttgttacg aagacggcac tggtgtccat gccagcaacg cccagaattt
     2341 gtgggttaac aaatacgatg tagaccatcg tcaggaaggt ggtgaacccg gcaatcactt
     2401 cagttcgtgc cgtagtacca tgttcgcgca gtttaaaaac gcgttccagc atcctctgac
     2461 cagaagtctg ggtggtatgt tgatgactca ttatctattt ccgaacaagg agggaaaatc
     2521 cgtcgctatc gtataccaaa atgcgacaat aggtgcgttt gtgagagact tttttattgg
     2581 atttacttat acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga
     2641 ttaaaaagga aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg
     2701 gtcgacagtg aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt
     2761 acgctcgatc ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc
     2821 gatattgttt cccttgaaca tggtgttacg ttagcgaaaa cagaagctga acacgtttac
     2881 cgtgcagaag tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctggagcg
     2941 ctcctgtcag cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg
     3001 cagcattcta tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc
     3061 tacgatattc agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg
     3121 aatgtaaatg tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt
     3181 atcgacgcag gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt
     3241 cacccgaaag tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt
     3301 ggttgggata ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg
     3361 ctcaaaggta agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga
     3421 ctttattttg cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc
     3481 ttcgcaaaag aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta
     3541 ttgtcgatta ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg
     3601 ccggaaaaga tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc
     3661 gttatcaccg agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag
     3721 ttgtttgaaa acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg
     3781 gcaggtgtgg tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct
     3841 gaaacagact ctttaagtta agcgg
//
LOCUS       DADLSA010000245         3839 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.248, whole genome shotgun sequence.
ACCESSION   DADLSA010000245 DADLSA010000000
VERSION     DADLSA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.248"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..458)
                     /gene="intS"
                     /locus_tag="JN691_004069"
     CDS             complement(<1..458)
                     /gene="intS"
                     /locus_tag="JN691_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HAY7245317.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(620..694)
                     /locus_tag="JN691_004070"
     tRNA            complement(620..694)
                     /locus_tag="JN691_004070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(659..661),aa:Arg,seq:cct)
     gene            complement(770..1702)
                     /locus_tag="JN691_004071"
     CDS             complement(770..1702)
                     /locus_tag="JN691_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAY7245318.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTPHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1996..2751
                     /gene="mlaA"
                     /locus_tag="JN691_004072"
     CDS             1996..2751
                     /gene="mlaA"
                     /locus_tag="JN691_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAY7245319.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2933..3763)
                     /locus_tag="JN691_004073"
     CDS             complement(2933..3763)
                     /locus_tag="JN691_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245320.1"
                     /translation="MDIGSNDTDHITIGIDNQEPIELPANYKDKELVRTIINDNIVEK
                     THDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLFTWYGKD
                     KKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSENARSASAWFE
                     SSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKFRRDNVK
                     EIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQAKYQNEINQLSALGVNFDDI
                     "
BASE COUNT          960 a          876 c          855 g         1148 t
ORIGIN      
        1 gcaccgcgat cttcaaatct gcggattact tccagcagtt gcatcggctc aatatcctga
       61 atctcaaggc cgccgatgat gggtaaaatg tcgtcatcaa acatttttgc aagttcagtc
      121 gcatacccta ctgaccagac ttgcttcttg tgttcgtacc attccttgta aattgcacta
      181 aatgaattgt tgttagacga agcctttttc gcctttaccg gatcgatgcc agccgagatg
      241 tctttcctcg cagtccatgc tttatccctt gcctcctgca aagtcataag cggatatttt
      301 ccgacggtca ggattttctc cttaccgtca atcttgtagc gaagctgcca tacctttttc
      361 ccggatacag ggacataaag gtacaggcca ttaccatcga gaaggcggta tggtttttct
      421 ttcggctttg ctgcttcaat ctgcttaacg gtgagcatgg gtaaaaatcc ggtgggtaaa
      481 attattttat ccacttttta cccgtcatgg agtgcggctg tcaacgatct gacgcgaacc
      541 atgacgaact gtgaatatac ggaaggcttg atattcaggg gattttgcgg actggtacgg
      601 atgggagcga actgataaat ggtgtcccct gcaggaatcg aacctgcaat tagcccttag
      661 gaggggctcg ttatatccat ttaactaaga ggacaatgcg gcatgagtat acccgctaat
      721 ggactgcggg gtaagtacgc tgccgctcga ttgcttaaac cctcgccatt tatgctgggt
      781 ttttatcatt tttcttaatg ttttccgcac gttctgcttt ttggcgtgct tctgctttac
      841 gcttgttgct catgtcgtta cgaatctgtg catgactcat taacgcgaag ataaaggtgc
      901 cgccgcagat gttccccgct aaagtaggta gtgcgaaggg ccagatgaaa tcgctccagt
      961 gcagcgtacc gttaaacacc agatagagga tttcaacaga accgaccacg atatgggggg
     1021 tgtcacccag ggcaataagc caggtcatca atataatcac cacaatcttt gccgcacctg
     1081 ctgcaggaaa catccatacc atagtggcga tcagccagcc ggaaatgatc gcgttggcaa
     1141 acatctcgct gggggtgttc ttcatcacat ccatgccgat tttgacaaat gcatcgcgag
     1201 tttcttcatt gaagataggc atatattcaa atgcccacgc agcaatacca gtcccgagaa
     1261 tattacccag cagcacgact ccccataacc gcataagtaa gccaacgttg ctcattgtcg
     1321 gtttttgcat gacgggtagt accgcagtca cggtgttttc ggtaaataat tgctggcggg
     1381 ccattatgac gataataaaa ccaaaggtat aaccgagatt ctccagcaag aagctgcccg
     1441 gcacaccttc cagttcgaca tgaaatatcc cttttgccag taacgaagcg cccatcgaca
     1501 gacccgccgc aatggctgac cacagtagcg ccattgcgtc gcgttctagc tctttttcac
     1561 catcctggcg gatatgctca tgaattgcca tcgcccggga ggggagtcgg tcttcatcta
     1621 tttctatttt tttgccgcgc tctttttctt cgctctcaac ttcaatttcg tcgctgtgtt
     1681 gatcaatttt gtcgttgtcc atggtctctt ctcttgaatt agcacgtata gctaaagcgt
     1741 agcggctttt ttgctcgcaa ctggcgggag ttactctgaa aatgtagaaa aggctgcgtt
     1801 tgcctttttc tgtttctata gaatcaagta gcctacaggg cggcgattac caggctatga
     1861 tcaaatcagc aaatcagggc gtctggacat cagttgacgt gctgttacaa tcgcccacac
     1921 ctaaacaggc ggatacggta tcgttccgtc atggatggca aactgcataa gccataaaaa
     1981 aacagggaga catttatgaa gcttcgcctg tcggcgcttg ctctgggaac tacgcttctg
     2041 gtggggtgtg cgagttccgg tacagatcag caagggcgtt ctgacccgtt agaagggttc
     2101 aaccgcacca tgtacaactt caacttcaac gtattagacc cgtatattgt tcgaccggtc
     2161 gctgtcgcct ggcgtgatta tgttccgcaa ccggcgcgta atggtttgag caactttact
     2221 ggcaaccttg aagaacctgc ggtgatggtt aactacttct tgcagggcga cccttatcag
     2281 gggatggtcc actttacccg ctttttcctg aacaccattt tggggatggg cggctttatt
     2341 gatgttgcag ggatggcgaa cccgaaactg caacggactg aacctcaccg cttcggtagt
     2401 acgcttggtc attatggcgt gggttatggg ccttacgttc agttaccgtt ctacggtagc
     2461 ttcacgctgc gtgatgacgg tggtgatatg gcggatggtc tttacccggt tctttcctgg
     2521 ctgacctggc cgatgtctgt gggtaaatgg acgcttgaag ggatcgaaac ccgcgctcag
     2581 ctgctggatt ccgatggtct gctgcgtcag tcgtccgatc cttatattat ggtgcgcgaa
     2641 gcgtacttcc agcgtcatga tttcatcgct aatggcggcg aactcaaacc gcaggaaaat
     2701 ccgaacgcgc aagcgattca ggatgattta aaagatatcg attctgaata agaaacaaat
     2761 aaaaaaggtg agtcgcaaaa ctcacctttt ttgttgttat ccctcaatta ccacaagtaa
     2821 aaggagataa accgacaatg acccggacgt aatgatgtaa aaatactgat gttcgtcact
     2881 gactatttcc gatattcagg cgttgaaaaa tcaaaaaact ggaaaaataa ttttaaatat
     2941 catcgaaatt aacacctagt gcagatagtt gattaatttc attttgatat ttcgcctggc
     3001 gattttgaag tcttttttca actttatctt cgagatcctt aaagtctttg tatttatttt
     3061 caggatggtc aacgtcaact ttgttagcga ataaagtaga aattatttct tttacattat
     3121 cgcgtctaaa ttttgcatcg tccacttgaa aattatatct tattgctcga cctaaacgtt
     3181 taaaaacttc gttaggttct ctcagtcttt ccccgttgag ctcgtttaca acctgatgat
     3241 cggaaccagt tgcaaatgat tctgccatga tagctttact gctttcaaac cacgctgaag
     3301 cactcctggc gttttcacta caatattggg tgagactatc accattaata cgaattcctc
     3361 tttgaatgtg gtagtcgtcc tgatcgtagt aaccgagaga taagtaatca tttccgtttt
     3421 tatttttaat tacaatagcg agagagtcgc cttttttatc tttaccgtac caggtaaata
     3481 agtccgttgg ttcgcttttc ccgctctcat caaattccgt taccgtatgt ctgagtttgt
     3541 gtgatatctt atcgaagata aaaccaggat ctccatcata tatttctttt aatgcgctga
     3601 agatcatttc cttgttattg atatcatgag tcttctcaac tatgttgtca ttaatgatag
     3661 tacggacgag ttctttgtct ttatagttcg caggtaattc tattggttct tggttgtcga
     3721 taccaatagt aatatggtct gtatcattgg agccaatatc aatataagta actgaggaat
     3781 tattaatatt aaatctataa ggatcgtcgt ttactcgtat ggcgtttttt ataagctca
//
LOCUS       DADLSA010000246         3825 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.249, whole genome shotgun sequence.
ACCESSION   DADLSA010000246 DADLSA010000000
VERSION     DADLSA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.249"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..1064
                     /gene="aldA"
                     /locus_tag="JN691_004074"
     CDS             <1..1064
                     /gene="aldA"
                     /locus_tag="JN691_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY7245321.1"
                     /translation="IQSDRPGENILLFKRALGVTTGILPWNFPFFLIARKMAPALLTG
                     NTIVIKPSEFTPNNTIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAGNPKVAMVSMT
                     GSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDSRVINSGQVCN
                     CAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAALERVEQKVARA
                     VEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVVAFDTLEDAIS
                     MANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHAGWRKSGIGGA
                     DGKHGLHEYLQTQVVYLQS"
     gene            complement(1106..2107)
                     /gene="gap"
                     /locus_tag="JN691_004075"
                     /pseudo
     CDS             complement(1106..2107)
                     /gene="gap"
                     /locus_tag="JN691_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            2296..2826
                     /gene="cybB"
                     /locus_tag="JN691_004076"
     CDS             2296..2826
                     /gene="cybB"
                     /locus_tag="JN691_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAY7245322.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3071..3244
                     /locus_tag="JN691_004077"
     CDS             3071..3244
                     /locus_tag="JN691_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245323.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(3316..3465)
                     /gene="hokB"
                     /locus_tag="JN691_004078"
     CDS             complement(3316..3465)
                     /gene="hokB"
                     /locus_tag="JN691_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAY7245324.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(3565..>3825)
                     /locus_tag="JN691_004079"
     CDS             complement(3565..>3825)
                     /locus_tag="JN691_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7245325.1"
                     /translation="YQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEFS
                     NISELKDAVTEYIEYYNSRRISLKLKGLTPNEYRNQTYMPRV"
BASE COUNT          990 a          858 c          936 g         1041 t
ORIGIN      
        1 ttattcaaag cgatcgtcca ggagaaaata ttcttctgtt taaacgtgcg cttggtgtga
       61 ctaccggcat tctgccgtgg aactttccgt tcttcctcat tgcccgcaaa atggctcccg
      121 ctcttttgac cggtaatacc atcgtcatta aacccagtga atttacgcca aacaatacga
      181 ttgcattcgc caaaatcgtc gatgaaatag gccttccgcg cggcgtgttt aaccttgtac
      241 tggggcgtgg tgaaaccgtt gggcaagaac tggcgggtaa cccaaaggtc gcaatggtca
      301 gtatgacagg cagcgtctct gcaggtgaga agatcatggc gactgcggcg aaaaacatca
      361 ccaaagtgtg tctggaatta ggtggtaaag caccggctat cgtaatggac gatgccgatc
      421 ttgaactggc agtcaaagcc atcgttgatt cacgcgtcat taatagtggg caagtgtgta
      481 actgtgcaga acgtgtttat gtacagaaag gcatttatga tcagttcgtc aatcggctgg
      541 gtgaagcgtt gcaggcggtt caatttggta accccgctga acgcaacgac attgcgatgg
      601 ggccgttgat taacgccgcg gcgctggaaa gggttgagca aaaagtggcg cgcgcagtag
      661 aagaaggggc gagagtggcg ttgggtggca aagcggtaga ggggaaagga tattattatc
      721 cgccgacatt gctgctggat gttcgccagg aaatgtcgat tatgcatgag gaaacctttg
      781 gcccggtgct gccagttgtc gcatttgaca cgctggaaga tgctatctca atggctaatg
      841 acagtgatta cggcctgacc tcatcaatct atacccaaaa tctgaacgtc gcgatgaaag
      901 ccattaaagg gctgaagttt ggtgaaactt acatcaaccg tgaaaacttc gaagctatgc
      961 aaggtttcca cgccggatgg cgtaaatccg gtattggcgg cgcagatggt aaacatggcc
     1021 tgcatgaata tctgcagacc caggtggttt atttacagtc ttaatgagtg aaagaggcgg
     1081 aggttttttc ctccgcctgt gcgcgtcaga gtttagcgaa tttttcgagg gtgcgaataa
     1141 gctgcgtgac gaagccatat tcgttatcgt atcaggcgac cgttttcacc agttgtaaat
     1201 cgcccacggc ggtaatttcc gtttgcgtgg catcaaacac tgaaccgaaa tggctgccaa
     1261 tgatatcgga agagactatt tcttcatcgg tataaccaaa tgactcgtta ttggtggttg
     1321 cttgtttaag tgcgttattc acctcttcgg cagtcacttt ttttccgaga atggacacca
     1381 gctcagtgac cgaacctgtt ttcaccggca cgcgttgcgc atgacctttc agtttgccgc
     1441 tcagttccgg gatcaccaga ccaatggctt ttgccgcccc cgtagtgtgg ggaatgatat
     1501 tttctgccgc tgcgcgtgaa gcacgtaaat ctttaccacg tgggccatcc accagtgact
     1561 gggtgccagt ataggcatga atggtcgtca tcgtgccgac ttctatcccg aaactgtcat
     1621 gcaaggcttt ggccatcggc gcaaggcagt tagtggtgca tgacgccacg gaaacaatgg
     1681 tgtcgttgcc atccagagtg tcgtcattaa cgttataaac gatggttttc atttcaccgg
     1741 caggagcgga aatcaacacc ttcttcgcac cagcatcaag atgcgcctgc gatttatcgg
     1801 cggaggtata aaagccagta cattcgacaa tgatttctgc ccctttcgct ttccacggaa
     1861 tatttttagc ctctttttcg gcgtaaaccg cgatactttt cccatcaacg ataagtgaat
     1921 cttccgtaaa atcaacgctc caggggaacg gaccatagtt tgaatcatgt ttcagcagat
     1981 aggcgagaat ttttggggaa gtaagatcat taatagcgac aacgtctatg ttgcttttga
     2041 cttcaagtaa acgacgcaac accagtcgac cgatacgacc aaaaccgtta ataccaactt
     2101 tactcatggt tttctcctgt caggaacgtt cggatgaaaa ttgatccttt ccaggcttag
     2161 accaggattg tgggatgggc aatctccatt cttacagtga aacgtaacgt actgaaaacg
     2221 ggtgaacaat atttaatgaa attttgagaa aagcccgtta tgttaacgga aaattatgtt
     2281 aaagcaggaa atgttatgga aaataaatac tcaaggttac aaatcagcat tcactggctg
     2341 gtctttttgc tggttatcgc agcgtattgc gcaatggagt ttcgtggttt cttcccacgt
     2401 agcgatcggc cgctcatcaa catgattcat gtttcctgtg gcatctcaat tctcgtgctg
     2461 atggtggttc gtcttttgtt aagactgaaa tacccaaccc cgccgattat acctaagccg
     2521 aaaccgatga tgacgggact ggcgcatttg ggacatttgg tgatttatct cctgtttatt
     2581 gcgctgccag tgattggttt ggtgatgatg tataaccggg gcaacccgtg gtttgcgttt
     2641 ggtttgacga tgccttacgc ttcagaggcc aatttcgaac gggttgatag cttaaagtcg
     2701 tggcatgaga cgctggcgaa tctgggatat tttgtcatcg ggttgcacgc tgcggctgca
     2761 ctggcacacc actatttctg gaaggacaac acacttctac gcatgatgcc gcgtaaacgt
     2821 tcctgaagga tatttaaaga aaacgcctgt actaaaaccg acccgtggta caggcgaaga
     2881 atacgggtct acatcggaag cgcctattat atttatttgt atgacaaata aaaccataat
     2941 ccttgcccat acgtccatct ggcttatttt taatcaattc acccgttctt tgatctcatc
     3001 aacggtatca aaataaacca gcgtataacg ttcaaatcgc acataagatt cgataaacca
     3061 tggaggttat atgaaaaaac tggcacttat tttgtttatg ggaacgcttg tttcctttta
     3121 tgccgatgcc gggcgcaaac cctgttctgg ttcgaaaggg gggatctcac actgtacggc
     3181 aggcggcaaa tttgtctgta atgatggttc tattagtgca tcgaaaaaaa catgcactaa
     3241 ctgaagtgta aaaggggtgc catgagaaaa tgaatctgct agtcagatgc gcggggaaaa
     3301 tccccgcgct tgcccttacc tggacgtgca ggccatgagc gcagcaacct ccttatcacc
     3361 gtcccggaac cgcagttcgt agagcgtttg tcgggtcagg agtgtgaatg ccagaatcgt
     3421 aatgcagata atgagcagac acaccaccag agggttgcgc ttcatagcct ttctccttgc
     3481 cggatggcgg gtaagaggct aacatctgaa ttgcttggtt cattcgttgg cctcggtttg
     3541 tactgacccc aaaaagttgg acagttaaac acgaggcata taggtctgat tccgatattc
     3601 atttggagtc agacctttta atttcaggct aattcttctg ctgttgtagt attcaatata
     3661 ttccgtaaca gcatccttca gttcgcttat attactgaac tcatcaagat aaaaacactc
     3721 cgactttaag gttccaaaga aacactccac cacagcatta tccagacaat tgccttttct
     3781 ggacatgctt tgtttaatac catgttcttt aaggatattt tgata
//
LOCUS       DADLSA010000247         3794 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.250, whole genome shotgun sequence.
ACCESSION   DADLSA010000247 DADLSA010000000
VERSION     DADLSA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.250"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..479)
                     /gene="fimB"
                     /locus_tag="JN691_004080"
     CDS             complement(<1..479)
                     /gene="fimB"
                     /locus_tag="JN691_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
                     /protein_id="HAY7245326.1"
                     /translation="MRNKADNKKRNFLTHSEIESLLKAANTGPHATRNYCLILLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     "
     gene            1933..2649
                     /gene="nanC"
                     /locus_tag="JN691_004081"
     CDS             1933..2649
                     /gene="nanC"
                     /locus_tag="JN691_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HAY7245327.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2669..3775
                     /locus_tag="JN691_004082"
     CDS             2669..3775
                     /locus_tag="JN691_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAY7245328.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSYAPMGMAGHVTFVHNGKAYVTGGVNQNIFNAYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
BASE COUNT         1188 a          750 c          778 g         1078 t
ORIGIN      
        1 agtcgcgtat ctattcccat attcgccaaa gcaaaaccac acgaatggcg taacatgtgc
       61 ggatgaatct ccagtgacaa cccggcatta ccaccagaag tcgagataat atggtaaaac
      121 tgttgccgag aaagcggatt ccccttacgt gataaaaata cccactcgct ctcagcatgc
      181 ggataagaag tacggatact caaccagttt tttaaagcct gaacttcttt gttcaatagc
      241 gggtgcgttg ttgaaaagcc tttttttaat cgatggatat atatacactt tgctttaaga
      301 tcaatatccg aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa
      361 agcaaaatca gacaataatt acgtgttgca tgaggcccgg tatttgctgc tttaaggagt
      421 gactcgattt cactatgggt aaggaagttc ctttttttgt tatcagcctt attcctcatc
      481 gttttccctt ataattacag acgcgcacta gctgtgctgg gttagcaata atccaacatt
      541 tttatcctga ttatgtttat aaaagcgaac gtttgcttaa taacttacgt aagtggacca
      601 gttcttctga gcgaacttaa atggagtagc gatagttact tataataact ttgccataca
      661 aacgccatgc tcttgcatgc tatgtacctt tatatattta tcaatctgat acgaatctaa
      721 taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat atgttctgta
      781 acaagtaaaa tcaacataaa aaattcagca aaatttaata tttgttcaac tagccgtctc
      841 aaatatgaca ttattagtta actgcatttg ccacaaaatg gctatttcat ggaacgcagt
      901 aatttcttag tggctattat catgctaatt cttagatccc tagcgattat tcctgcctgg
      961 ttcgtcagct taggccatga gattgttaca ttccttttta tcctggagat atgggggaac
     1021 gcaggcgacg ctttttcatc tacatccgtt ggtaaatata gctttggtca ctacgactta
     1081 agccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa gtgaaacatc
     1141 atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt gaccagttac
     1201 aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg atggcgggta
     1261 aacccttatt ttcaccactc tcactgagtt agcttgcaaa gctttctttg tttagattct
     1321 aaataaattt tttataaaac atatagttaa aagaaaatct ttagtttttg aagaccaccg
     1381 caagtgttcg tctggcttca catggcatct tcttcttaga aaaagatcga catattttgt
     1441 gacgcgaatt gcaaatctgg ttttgttgta tggattgcgt gatttttgat ctggtataac
     1501 aggtataaag atgcaccacg atagtcaatg agactgggca tctcgtaatc tatggcaaac
     1561 atcacttcag ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa aggaatatga
     1621 aaatgagatc aacagacact ctattttatg actctgggta aaatgggttg agtaagtgat
     1681 ataacttacg aatattcaaa tcaattaaaa atcagaagag attttatact caggtattta
     1741 atctggatct ctgtttattt aaataatgtg aaaagagaat tttcacagga gaccttatac
     1801 aaaaaatata aaatacagtt acccgttgcc aaagacacta taagcctggc aaaaaaatat
     1861 tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt atcctacaaa
     1921 tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact gtgcttttcg
     1981 tccccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg tagtggaagc
     2041 cacgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg gtgggcaagc
     2101 atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc cgcactcaat
     2161 gatgttcagg ttgaagttaa ttacgcaatt aaacttgatg atcaatggac ggtgcgcccg
     2221 ggaatgttaa cgcattttag cagcaacggc acacgctacg gaccctacgt aaaactgtcc
     2281 tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg gaaagcttac
     2341 cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg ggatggatat
     2401 gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct atacagcaaa
     2461 cagaatgatt atcgctatgc aaaccacaag aaatgggcaa cggaaaatgc ctttgttcta
     2521 caataccata tgacgcccga tattacgcca tacatagaat atgactacct tgaccgccag
     2581 ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg tgtgtcattt
     2641 aaactgtagt agacaggaga cagtcacaat gaataaaact ataatggcgc ttgctatcat
     2701 gatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc catttaaaag
     2761 tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg caggtacagc
     2821 atggtacaag ctagatacac aggccaaaga taaaaaatgg actgcgttag ctgcattccc
     2881 tggtggaccg agagagcaag caacctctgc atttattgat ggcaatctgt atgtgtttgg
     2941 cggcattggc aaaaacagcg agggattgac tcaggtattt aatgacgtac acaaatacaa
     3001 ccccaaaacc aatagctggg ttaaattgat gtcgtacgcg ccgatgggca tggcgggtca
     3061 tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc agaatatctt
     3121 caatgcctat tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta tagataaaat
     3181 caacgcccac tattttgaca aaaaagcaga agattatttc ttcaataagt ttctgttgtc
     3241 ttttgatccc tcaacacagc aatggagtta cgctggcgaa tcgccctggt acggaacggc
     3301 tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg aagccaaacc
     3361 aggattgcga acggatgccg tatttgaact tgatttcacc ggtaataatt taaaatggaa
     3421 taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg ggataagcaa
     3481 tgattctctt atatttgccg gaggggccgg attcaaaggt tcacgagaaa attaccagaa
     3541 cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcactgata ttcatctttg
     3601 gcataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct acggagtatc
     3661 attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg gcaaagcggt
     3721 gacggattca gttttaattt ctgtgaagga taataaagtc acagtacaaa actaacgctt
     3781 cagggccccg gtaa
//
LOCUS       DADLSA010000248         3625 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.251, whole genome shotgun sequence.
ACCESSION   DADLSA010000248 DADLSA010000000
VERSION     DADLSA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.251"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            77..904
                     /gene="nadE"
                     /locus_tag="JN691_004083"
     CDS             77..904
                     /gene="nadE"
                     /locus_tag="JN691_004083"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAY7245329.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVAYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            1134..2021
                     /gene="cho"
                     /locus_tag="JN691_004084"
     CDS             1134..2021
                     /gene="cho"
                     /locus_tag="JN691_004084"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAY7245330.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(1981..2556)
                     /gene="ves"
                     /locus_tag="JN691_004085"
     CDS             complement(1981..2556)
                     /gene="ves"
                     /locus_tag="JN691_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAY7245331.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGELSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(2759..3244)
                     /gene="spy"
                     /locus_tag="JN691_004086"
     CDS             complement(2759..3244)
                     /gene="spy"
                     /locus_tag="JN691_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAY7245332.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEECRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT          920 a          858 c          897 g          950 t
ORIGIN      
        1 cccgctgtcg cttgtcgttt cagtagcaac gggttagctt taaggaagtt ttgtcttttc
       61 tgtctggagg ggttcaatga cattgcaaca acaaataata aaggcgctgg gcgcaaaacc
      121 gcagattaat gctgaagagg aaattcgtcg tagtgtcgat tttctgaaaa gctacctgca
      181 aacttatccg ttcattaaat cactggtgct cgggatcagc ggcggtcagg actccacgct
      241 tgccggaaag ctgtgccaga tggcgattaa tgagctgcgc caggaaactg gcaacgaatc
      301 actgcaattt attgccgtac gcctgcccta tggtgttcag gctgacgaac aagattgcca
      361 ggatgccatt gcctttattc aaccggatcg cgtattaacc gttaatatca agggcgcggt
      421 attggctagc gagcaggcat tgcgggaagc aggcattgaa ctgagcgatt ttgtccgtgg
      481 caatgaaaaa gcgcgtgagc ggatgaaagc acaatatagc attgcgggta tgaccagcgg
      541 tgtcgtggtg ggcaccgatc atgcagcaga agccattacc ggattcttca ctaaatatgg
      601 tgacggcggt acggatatta atccgctgta tcgtctcaac aaacgccagg gtaaacagtt
      661 actgacggca ttaggttgcc cggaacacct ttataagaaa gccccaactg ccgatctgga
      721 agatgatcgc ccttctctgc cagatgaagt ggcactcggc gtggcctatg acaatatcga
      781 cgactatctg gaagggaaaa acctacctga acaggtcgcc agaacaatag agaactggta
      841 tctgaaaacc gaacataaac gccgtccgcc aattaacgtt ttcgatgatt tctggaaaaa
      901 gtaataattt gcacctattg gattgtgcga aaaagagtaa tttgttcacg ccggatgcgg
      961 cgtgaacgcc ttattcgacc tacaaaacta tgcaaattca atacattgca ggagtcgaat
     1021 aggcctgaca ggcgtagcac gtcagacggt gtaacctttg tcatcaaccc gcctcttttt
     1081 taatcgcctc ccgcctgtta cactggatag ataaccagca ttcggagtca acagtggtac
     1141 ggcgtttaac ttctccgcgg ctcgaatttg aagctgcggc aatttatgaa tatcccgaac
     1201 atttacattc attccttaat gacttaccca cccgaccagg ggtgtatctg tttcatggcg
     1261 aaagtgacac catgccgctc tatatcggca aaagcgttaa tatccgcagt cgggtgcttt
     1321 ctcatttacg caccccggat gaagccgcca tgctacggca atcccggcga attagctgga
     1381 tctgcaccgc aggcgaaatc ggcgctctgc tccttgaagc gcgattaatc aaagaacaac
     1441 agccgctgtt taataaacgg ttgcgccgca atcgccagct ctgtgccctg caattaaatg
     1501 aaaagcgcgt cgatgtggtg tatgccaaag aggtggattt ttcacgagcc cccaacctgt
     1561 ttggcctgtt tgccaatagg cgcgcagctt tgcaagcatt gcagagcatc gctgatgaac
     1621 aaaaactttg ttatggcctg ttgggactgg aaccgttaag tcgcggtcgt gcatgttttc
     1681 gttcagcgct aaaacgttgc gccggagcat gctgcggtaa agagagccat gaggaacatg
     1741 cgctacgctt gcgccagtct ctggagcgtt tgcgggtggt gtgttggcct tggcaaggtg
     1801 cggtggcact gaaagaacag cacccggaaa tgactcaata tcatattatt caaaactggc
     1861 tgtggctggg ggcggttaat tcgctggaag aagcgacaac gttaattcgg acacccgccg
     1921 ggtttgatca cgacggttat aaaattcttt gtaagccgct gctttccggt aactatgaaa
     1981 ttactgaact tgatccggcg aatgaccagc gagccagttg atttcactga acaacagttt
     2041 accttgcggt tgtaaaagcc gcagggtgtg cctgccatcg aaccagcacg cgccctggtc
     2101 ggtagtgagc agtttgtcgc ccagttgcca cgccccgtta atcacaaaca ccaccccgcc
     2161 gcgtgagcca aaggtagtaa acgtacgttc ggcaattctg actttcgcct tacagacatc
     2221 caggcgcgtc ataatattga aatccatcga catctgccct gccgtcagtt tcgcttttac
     2281 cacctggtcc gccgtaaagg caaaaggctg taacggtttt aaggtatggt taaagcgatc
     2341 tgcgctttca aggagcatct cgccgccttc cagcaacgtc actatccttt ccatgccggg
     2401 aaacaaagaa agctccccat tcgccgcgat ggatgcaatg ctggcacgcc agtaaaaatc
     2461 acgttttgcc ggagggaaag tgcaaatttc gcgcgtttca ccggcagcgt ttcgccacag
     2521 attcaccgac attttacgca tatcaaagta ttccatactc gctcccggcc cgttcttttc
     2581 attgttatcg ttcaggtaac cgtggatggt tatcggcaac tcgggtgatt tacgttagtg
     2641 gtgatcaggc tttttttaca actgtttgag aagagaaaag aaaaccgccg atcctgtcca
     2701 ccgcattact gcaaggtagt ggacaagacc ggcggtctta agttttttgg ctgaaagatt
     2761 attcagcagt tgcaggcatt ttaccttttg ccgctggacg ttctgtcaga cgcttctcaa
     2821 aattagcatt aaattgcttt ttctgttccg gcgtcagaat gttgtaaatt ttgttctggg
     2881 tttccatgtg cgccagcatg ttagctttgc gctgttcttc catttttgcg atctgcgctt
     2941 cagcttttgc tttatcgaag gtatcgctgg caatgatgtc atgcattgcg cggcattctt
     3001 ccagcggcgg acgtttcatc tggtcacgct ggcctttcat gatttcgtgg atctgctgtt
     3061 tctgcgcgtc ggtcaggttc aggtctttga acatcatgtc ctgatgcgga ccgaacttgc
     3121 ctttgtggtg catcatcggc ttcgcgtcag ccggtgctgc ggtagtggtg tctgcggcat
     3181 gggccaagtt agccgcgcca agagccaggg tagaggcaac aaacagtgca gttaatttac
     3241 gcatattcta tatccttcct ttcagttatt tattacggct ttcttaagta gcgtgccgtg
     3301 ttgacgagat taactttact gactttagcg tcaattaatc agagcaacgg taaaacaatg
     3361 aaagtgtaaa aaacactttt gcgccaatta tggagaaaaa aagaaaattt gatggagagt
     3421 gatgagagaa tattacaaca cgatgatttt gcagagatta tgaagaacta taccggatga
     3481 ctggtgataa ataaagcaaa taaccaggat taatctgtat taatttataa gaaagcaact
     3541 taatacccgc agaatgattt ctgcgggtaa gtattagctt attttttcga gcattaatcc
     3601 cgcgcgtaat cccaacgcta ccaac
//
LOCUS       DADLSA010000249         3614 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.252, whole genome shotgun sequence.
ACCESSION   DADLSA010000249 DADLSA010000000
VERSION     DADLSA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.252"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..796
                     /locus_tag="JN691_004087"
     CDS             <1..796
                     /locus_tag="JN691_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7245333.1"
                     /translation="WLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            807..1784
                     /locus_tag="JN691_004088"
     CDS             807..1784
                     /locus_tag="JN691_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7245334.1"
                     /translation="MKRAPLITGLLLISTSCAYASTLSSTNWQYACSCSAGKAVKLVY
                     MVSPVLTTTGHQTGYYKLNDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYS
                     AATQTGVCQGDTSRYGPVNIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGS
                     PSTGDFHDLVKLSIQGNLTAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVD
                     FDITYDCGDTSKIKNSLQMRIDGTTGVVDQYNLVARRRSSDNVPNVGIRIENLGGGVA
                     NIPFQNGILPVDPSGHGTVNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1827..2687)
                     /gene="rsmI"
                     /locus_tag="JN691_004089"
     CDS             complement(1827..2687)
                     /gene="rsmI"
                     /locus_tag="JN691_004089"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAY7245335.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGALLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2752..>3614
                     /locus_tag="JN691_004090"
     CDS             2752..>3614
                     /locus_tag="JN691_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY7245336.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGT"
BASE COUNT          923 a          937 c          884 g          870 t
ORIGIN      
        1 ctggttaccg cgtagccggg tttcctatca gatgaccagc cagaaagatc gcccaaccca
       61 acatgaaatg cgtcttgatg gctcactgct ggatgatggt cgcctgagct atagcctgga
      121 acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt accgttcacc
      181 ttatggaacc ttcagtgccg gatacagtta cggtaatgac agcagccaat acaattacgg
      241 cgttaccggc ggcgtggtta tccatcctca tggcgtgacg ctctcgcaat atctgggcaa
      301 cgcttttgcg cttatcgatg ctaacggggc ttctggcgtg aggatacaaa actatccggg
      361 gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt atcaggaaaa
      421 ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgaac aaacaacaca
      481 gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca atatcggtta
      541 tcgcgtactt gttacagtca gcgatcgcaa cggtaaaccg ttgccctttg gcgctcttgc
      601 cagcaacgat gatacggggc aacaaagtat cgtcgatgag ggcggcatac tatatctctc
      661 tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg cagatcaaca
      721 atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat tacaaggcac
      781 agcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca ggacttttgt
      841 tgatatccac atcctgcgct tatgcctcaa cgctaagtag cactaactgg caatacgcct
      901 gttcctgctc tgcgggtaag gcagttaaac ttgtctatat ggtcagcccc gtacttacca
      961 ccactggaca tcagacagga tattacaaac tcaatgacag cctggatatt aaaaccacat
     1021 tacaggcaaa cgacattcca ggactcacaa ccgaccaggt tgtctctgtt aacacccgat
     1081 tcacacagat aaaaagcagc accgtatatt ctgctgcaac ccaaacgggt gtttgccagg
     1141 gtgacacgtc tcgttatgga cccgttaata ttggtgcaaa taccaccttt accctgtatg
     1201 tcaccaagcc atttctcggc tcgatgacca ttccgaaaac ggatattgcc gtcattaaag
     1261 gcgcgtgggt cgatggaatg ggaagcccgt ctacaggtga cttccatgat ttagtcaagt
     1321 tatcgattca gggaaatctc accgccccac agtcgtgcaa aattaatcag ggcgatgtta
     1381 ttaaagttaa ttttggattc atcaatggtc agaagtttac cacccgcaat gccatgccag
     1441 acggttttac tccagtagac tttgatatca cttatgactg tggtgatact tcaaagatta
     1501 aaaactcgtt gcaaatgcgc attgacggta caactggggt agtagaccag tacaacctgg
     1561 tcgccaggcg aagaagttca gacaatgtgc ccaatgtcgg tattcgtatt gaaaatctcg
     1621 gcggcggagt tgcaaatatt ccttttcaga acggtatcct tcccgttgat ccttccgggc
     1681 atggcaccgt caatatgcgc gcctggccag ttaatctggt cggtggtgag ctggaaacag
     1741 gaaaatttca gggcacagcc accattaccg tcatggtgcg gtaaaagaag acgttgcgtt
     1801 ttgattaacg caacgcttcg gcactgttac ccctgctgct ccagcgcata cttatacagc
     1861 gcatttttct tcacgccatg aatttctgcg gccagcgccg ccgctttttt cagcggcagt
     1921 tctgcctgta gcagcgccag cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct
     1981 ttatgacctt cgacaatcag caccatctcg cctttgcgac ggttttcatc ttcctttacc
     2041 cacgccagca gcgcgccaac gggcgcgccg tgaatggttt cccaggtttt ggtcagctca
     2101 cgcgccagaa ccacgtagcg ggattcgcct aataccgcaa cgatatcttc caggctatct
     2161 aacagacggt gggtagattc ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt
     2221 agcgcatcac ggcggccttt tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca
     2281 gatggtaaac ccgcggcgct taacgcagtg atagcagcac acggtccggg tagcggcacc
     2341 acacgaatcc ccgcttcacg gcaggtacgc accagatggt agccaggatc gttaattagc
     2401 ggcgttccgg catcggaaac cagcgcaatg ttttgcccct cttgcagctt cgccagcagc
     2461 gtttcggctt tttgttgttc gttatggtcg tgcagcgcaa acaaccgggc attaatccca
     2521 aaatgttgca gcaataaacc ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc
     2581 tgtaatacct ctaacgtacg ctgggtgata tccgccagat tgccgattgg cgtcggtaca
     2641 atgtaaagct ggccctgaga attatccgcc gattggtgtt gtttcattgt gtcgtccgta
     2701 ttgccgattt aatattgagc attgcgtaaa aaaaatatca ctggatacat tatggtaccc
     2761 tcaacatttt ctcgtttgaa agccgcgcgt tgtctgcctg ttgttctggc agccctgatt
     2821 ttcgccggtt gtggcaccca tactcccgat cagtccactg cttatatgca gggcacggcg
     2881 caggctgatt ctgcctttta tcttcagcag atgcagcaaa gctctgatga taccaggatc
     2941 aactggcaat tactcgccat tcgtgcactg gtgaaagaag gtaaaaccgg gcaggcggtt
     3001 gagttgttta accaactacc gcaagaactg aacgattctc agcgtcgcga gaaaacactg
     3061 ctggcggcag agattaaact ggcgcagaaa gattttgctg gcgcgcaaaa cttgctggcg
     3121 aaaatcacac ctgccgattt agaacaaaac cagcaagcgc gttactggca ggcaaaaatc
     3181 gatgccagcc aggggcgtcc ttccattgat ttactgcgcg cgttaattgc tcaggaaccg
     3241 ctgcttggcg cgaaagaaaa acagcagaat attgatgcca cctggcaggc gctctcctcc
     3301 atgactcagg aacaggcgaa tacgctggtg atcaacgccg acgaaaatat tctgcaaggc
     3361 tggctggatc tgcagcgcgt ctggtttgat aaccgtaacg atcccgacat gatgaaagcc
     3421 gggatcgccg actggcagaa acgttatccg aacaatccgg gcgcgaaaat gctgccaacg
     3481 cagttggtta acgtaaaagc gtttaaacca gcctcgacca acaaaatcgc cctgctgttg
     3541 ccgctgaatg gccaggcagc ggtatttggt cgcactattc agcaaggctt tgaagccgcg
     3601 aaaaatatcg gcac
//
LOCUS       DADLSA010000250         3589 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.253, whole genome shotgun sequence.
ACCESSION   DADLSA010000250 DADLSA010000000
VERSION     DADLSA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.253"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            341..538
                     /locus_tag="JN691_004091"
     CDS             341..538
                     /locus_tag="JN691_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAY7245337.1"
                     /translation="MNAHDIEPTILRATGNTHPLDVTFIHEEDLATPWKPEQSSVYAH
                     VNPYGIFELDMETRLPIEVVA"
     gene            complement(620..2329)
                     /locus_tag="JN691_004092"
     CDS             complement(620..2329)
                     /locus_tag="JN691_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAY7245338.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2762..3481)
                     /locus_tag="JN691_004093"
     CDS             complement(2762..3481)
                     /locus_tag="JN691_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HAY7245339.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1068 a          723 c          638 g         1160 t
ORIGIN      
        1 cttgatacaa ctggtctggt atatacagtt tctttgagtg cggttacgtc cacagaggta
       61 acggtaaaag taaccccttg tcgggaccac tccgaccgcg cacagaggca ttttcatcgc
      121 tgacaggccg cattgcctcg cagggctgga ggaggatcgg tgatatccgg acgaccgtac
      181 cggcttacgc cgggcttcgg cggttttact cctctgccat gtgcaacaac ggagtgccgc
      241 cttacatgcc gctggcgcgg catatcctgg taacattttt gttgatacca ccttccggcc
      301 ttatcctgac cagtgggctt tcctggcatc gatgataccg atgaacgcgc atgatattga
      361 gccgacgata ttacgggcaa ctggaaatac tcaccctctg gatgtgacct ttatccatga
      421 agaagatttg gccacaccat ggaaacctga acaatcatca gtttacgccc atgtaaaccc
      481 ctacgggata tttgaactcg atatggaaac aaggttgcca atcgaggtgg ttgcctgaaa
      541 gttggcagtt acccggtcac tacaccttgt tggagtgaac atatcaaaaa caggtaatta
      601 cacaatttaa attacagtct cataaaaaat gattaaatct atttattatt tcaataaacg
      661 tttttttcat cttctttgaa aattgttctg ctatagtatc cggttccaga ttgttctctt
      721 ttattattct acaatactct aatattgttt ctttgtctct atttactaca gatacaaata
      781 caccaggaat accatcttta ttagttgcgg acagcatttc gaccaatcgt cttgtggtaa
      841 aactatagtt ttttagaata ttacaaaagg cacgtattgt ttcggtatgg ccattataaa
      901 atgccataaa aagtcctgat gtaccgttgg gattttttat ccataataat tcatctatat
      961 attcctggtg aggggggagt atttttagta tatctccata cgccataatg gcctttgtgt
     1021 gcccattttg aagtgccata aacaacccat gctttacttt caataattct ttaatttgtt
     1081 cactactaag cattttctta ttaagcagac ttccgaacat acgaatacta tctgcatgtc
     1141 cattctgcaa tgcaaaaaat aacccaggta aatcatggct gtctttcgca gctaatagat
     1201 ataatctctt atcggaagtt aacggagttt taaagactat ttccccatag gcttttattg
     1261 tgtctacatg tccgttcata aaagacatgc ataaacccgg cacattgttt gcccctaccg
     1321 cagcaagcaa tgtaagtatt gtttcttgac taagattgca catatttaat atcttaccat
     1381 atacttgaat aatgtctgaa catccgtttt gaagtgctat ataaaatgca ggagtaccat
     1441 cagagcttct cgtggccaga aggttcacca gtttagattg agaaatttca ccatttttgt
     1501 atttttccat cagtataaat aatacttctg tcagattctt tgttatcgca tgatatataa
     1561 ctctggaatt gatcaattca tctggtaaaa gtttcactgt gtttgttaga ggggaattat
     1621 gcctatcttc aaatattacc tgcccgggtt gctccagacc ataaattttc caataaaaat
     1681 catcaaaaaa atccttagcg gataattgac tcagttcgaa atggctatca caagtaaata
     1741 cagtacgttt atgagtaaca gttcgattag gatcgaaaaa ctgaacaacc catttttcac
     1801 tttcttgtac gcgctttatt ttcaacccta acgccatttg gtggtttaat gtgcatgctg
     1861 ataatgttct gacaaagtct ccattttttt tcatgttata aaaaaaatta gtaataactg
     1921 ttccccactt tttattttcc acaaaataaa tataagctgg tgattttgaa aaatcttccg
     1981 ttttttccaa acatacattt tttgcaatag catcaggggt gctaagaagc tgatagttta
     2041 ccttcccatt tgtttccata aaattcattt cccaatagga agctaggtga cggcaaacta
     2101 tttgtcttcc atatatatct tttgccactc catttatatt taccttcaca tttctctttt
     2161 ctgaaaaata aggtgctgtt ttattcttaa actgaattag ttcactggag tttgagaccc
     2221 tcataataga acttgttatt gttctattat ctctgttaac agacatatct gttgataacg
     2281 aattttttgt tgaaaatata ctagatgaaa atgttttatt taacggcata gaagacaacc
     2341 atagagatgt tataatacta ttagattgca gttactaata gtaaaagcct ccttacaagg
     2401 aggtagctta ttcagaatgc tcgctataag atagcgagct cgtcaacgat acagatgatc
     2461 tgattatatt gtgcaaaagg agaacaggaa aataggcact ggaaaagatg aaggcctgat
     2521 gggagcatta actaatgcaa aatccgacag gttaataacc cgaaaagcaa tcaaacgact
     2581 caaacaggag atgcacgctg aagtggtgga caactgttcg gatttcagat gactagctca
     2641 gcagtttatg agccgaatgc tctgtcacgg gcaggaatca ggctgcatac gctgtgccaa
     2701 atctaatcgt agttattcta accagttttc aagctacaag gtggtgtagc tggcatcttc
     2761 tctactctat catcaaacga taaaatggtt cctctcgtaa catttgctct tgcctttcac
     2821 ttccatctcg atcgcttcgt agttcattac gatagctgac atatttccag tcttcaggtc
     2881 taacatctga cgccggatac tccccgggag cgattttgct tcttaataga ttagactcga
     2941 gacacattat aaattgtcgt attttatgaa gtaataaagc actatattgc gagcattcct
     3001 gatctgattt tacatatagc gtaaactgag ctcctatccc cacacgagat tgctgagaaa
     3061 ctcgattcat atcagttatc ttccatttat ctataggact gtcctctgaa aataataaac
     3121 cagacaaaat ttggaatgcc agaggaactt gctccctagc tatactaata tgaaacttgt
     3181 ccccaacaaa atccccctta ctctgaggat tttcacgaca tgcatgaata aacacatctc
     3241 cactatggtt cacaatctgg aatccttggt acatgacaga aaaaaattga catatatcag
     3301 agtaagttgg cacgttcgat gctactttga aatagtacgc tgggtattgc cgtatttggt
     3361 tgtacaatat attgaagtta tttttcaatc tttcattcgc agaaagcatc tgcggtattt
     3421 cagacctcac aacattcaaa gaatctaaat ttagttttag acagggcttt tttatgggca
     3481 tagaaaacgt cctctataaa atagatatat tatcttatat tgattgttta tcatctttta
     3541 taaaacgata cattatatgt aatgtatgta agcgtaaggg tcttctccg
//
LOCUS       DADLSA010000251         3588 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.254, whole genome shotgun sequence.
ACCESSION   DADLSA010000251 DADLSA010000000
VERSION     DADLSA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.254"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            1..372
                     /locus_tag="JN691_004094"
                     /pseudo
     CDS             1..372
                     /locus_tag="JN691_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427357.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            381..809
                     /locus_tag="JN691_004095"
                     /pseudo
     CDS             381..809
                     /locus_tag="JN691_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
     gene            870..3293
                     /locus_tag="JN691_004096"
     CDS             870..3293
                     /locus_tag="JN691_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HAY7245340.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKTVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAI
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTKMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPIPAYTSGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            3304..>3588
                     /locus_tag="JN691_004097"
     CDS             3304..>3588
                     /locus_tag="JN691_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit B"
                     /protein_id="HAY7245341.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVD"
BASE COUNT          984 a          903 c          898 g          803 t
ORIGIN      
        1 tcattttcct ccagccagtc accagcgaaa aattcgaacg taaaccgatt tactggatcc
       61 tgagtgaagt cgcgaaacgt cttggacctg acgtctatca aaagttcaca gaaggtcgca
      121 cgcaggaaca atggttacaa catctgtacg ccaaaatgct tgccaaagat ccggcgttac
      181 cgtcttacga cgaactgaaa aaaatgggta tctataagcg taaagatccc aatggccatt
      241 ttgtcgccta caaagcattt cgtgacgacc ccgaggcaaa tccacttaaa acgccttccg
      301 gtaagattga aatttattcc agcaagctgg cggaaattgc ccgtacctgg gaactggaaa
      361 aagatgaagt gataagccca ctgccagttt atgcctcaac ctttgaaggc tgggactccc
      421 ctgaacgtag aaccttccca ctgcaactgt ttggtttcca ttacaaatcc cgtactcact
      481 cgacctacgg caacattgat gtcctgaagg cagcctgccg tcaggaggtg tggatcaacc
      541 ctatagatgc acagaaacgt gggattgcca acggcgatat ggtgcggttg tttaaccatc
      601 gtggcgaagt tcggctacca gccaaagtaa caccacgcat tctccctgga gttagcgcta
      661 tgggccaggg agcctagcac gagcccaata tgtctggcga taaaatcgac catggcggat
      721 gtgtgaatac gctaaccact ctgcgccctt caccactggc gaagggaaac ccgcagcaca
      781 ctaatctggt cgagatcgaa aaaatataac ccactacaac cataaattct ggcatgacat
      841 tttgtttgaa aagcaataag tgagtaatga tgaaaatcca taccacagag gcgctcatga
      901 aggctgaaat cagccgtaga agtctgatga aaacctccgc acttggcagt ctcgcgctgg
      961 caagcagtgc tttcactctg ccattttccc aaatggtccg ggcggcagag gctccggtag
     1021 aagagaaaac ggtctggagt tcctgcaccg ttaactgcgg gagccgctgt ctgctacgtt
     1081 tgcatgtgaa agatgacacc gtgtactggg tggagtctga tacgacaggt gacgacgttt
     1141 acggtaatca tcaggttcgg gcatgtttac gcgggcgctc tatccgccga cggatgaatc
     1201 atcctgacag gttgaaatat cccatgaagc gcgtcggcaa gcgcggtgaa ggtaaatttg
     1261 aacggataag ttgggacgaa gccctagata ccatcagtga taatcttcgg cggatcctga
     1321 aagattacgg caatgaggct gtacatgtcc tgtacggaac aggcgtagat ggcggaaata
     1381 tcaccaactc gaacgtcccg taccgtctga tgaactcttg cgggggtttt ctcagtcgct
     1441 atggcagcta cagtaccgca cagatcagtg ccgcaatgag ttatatgttc ggtgccaatg
     1501 atggcaacag cccggatgat atcgccaata cgaaactggt cgttatgttc ggaaataacc
     1561 cggcagaaac gcggatgagc ggcggtggtg tcacttacta cgtcgagcaa gcccgcgaac
     1621 gttcaaacgc acgcatgatc gtcatcgatc cacgctataa cgacacagct gccgggcgtg
     1681 aagatgaatg gctgcccatt cgccctggca ccgatggcgc actggcctgt gcgattgcct
     1741 gggtactgat tactgaaaac atggtcgatc agccatttct cgacaaatat tgtgttggtt
     1801 acgatgaaaa aacgctgccc gccaacgcac cacgtaacgc gcattataaa gcctatattc
     1861 tgggcgaagg gcctgacggc atagctaaaa cgccggaatg ggcagcaaaa atcaccagca
     1921 tcccggcaga aaaaattatc cagttggcac gagagatcgg ttcagcaaaa cctgcttata
     1981 tttgtcaggg ttgggggcca caacgacact ccaacggcga acaaacgtcc cgcgctattg
     2041 ccatactttc cgttcttacc ggtaacgtcg gcataaacgg cggcaactca ggtgtacgcg
     2101 aaggtagctg ggatctgggg gtagaatggt tcccgatgct cgagaatcct gttaaaacgc
     2161 agatttccgt ttttacatgg acagatgcca tcgaccatgg tacgaaaatg accgcgaccc
     2221 gtgatggtgt tcgtggaaaa gaaaaactgg atgtccccat caagttttta tggtgctacg
     2281 ccagtaacac attgatcaat caacatggcg acatcaatca cacccatgag gtgcttcagg
     2341 atgacagcaa gtgcgagatg attgttggca ttgaccactt catgacggcc tcggctaagt
     2401 attgcgatat cctgttgcct gacctgatgc caacagagca agaagacctt atctctcatg
     2461 aatctgcagg gaatatgggc tatgtgatcc tcgcccaacc cgcaacctca gcaaaatttg
     2521 aacgcaaacc catctactgg atgctgagtg aagtcgccaa acgcttagga ccagacgttt
     2581 atcaaacctt tactgaaggt cgcagtcagc atgaatggat caaatatctc catgcgaaaa
     2641 cgaaggaacg taaccctgag atgcccgact acgaggagat gaaaacgacc gggatcttta
     2701 agaaaaaatg cccggaagaa cactacgtcg ctttccgcgc attccgtgaa gatccacagg
     2761 caaacccgtt gaaaacacct tcggggaaaa tcgaaattta ttctgaacga ctggcgaaga
     2821 ttgcagatac ctgggaattg aaaaaagatg aaattattca tcccattcct gcgtatacct
     2881 caggttttga tggctgggac gatcccctgc ggaaaaccta tccactgcag ttaacgggct
     2941 tccactacaa agcgcgtacc cactccagct acggcaatat tgatgtgtta cagcaggcct
     3001 gcccacaaga ggtgtggatc aaccccattg atgctcaggc acgcggtatc cgtcatggcg
     3061 ataccgtgcg ggtatttaac aataatggag aaatgctgat tgccgcaaaa gtgactccgc
     3121 gtattctgcc tggcgttacc gccatcgggc aaggtgcgtg gcttaaagcg gatatgtttg
     3181 gtgaccgggt cgatcacggc ggcagtatca atattctgac ctctcaccgc ccttcaccgc
     3241 tggcaaaggg aaacccgtcg cacagcaatc ttgtccagat cgaaaaggtt taaggagtag
     3301 cccatgacca cacaatatgg attttttatc gattccagcc gctgtactgg ctgtaaaacc
     3361 tgcgaactgg cgtgcaaaga ttttaaagat cttggcccgg aagtcagttt ccgccgtatt
     3421 tatgaatatg ctggcggcga ctggcaggaa gataatggca tctggcacca gaacgtgttc
     3481 gcttattatc tctccatctc ctgtaaccat tgcgatgacc ccgcatgtac aaaagtctgc
     3541 cccagcggcg caatgcataa gcgtgaagat ggctttgtgg tggttgat
//
LOCUS       DADLSA010000252         3543 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.255, whole genome shotgun sequence.
ACCESSION   DADLSA010000252 DADLSA010000000
VERSION     DADLSA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.255"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..702)
                     /gene="tqsA"
                     /locus_tag="JN691_004098"
     CDS             complement(<1..702)
                     /gene="tqsA"
                     /locus_tag="JN691_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAY7245342.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALN"
     gene            1114..1479
                     /gene="mdtJ"
                     /locus_tag="JN691_004099"
     CDS             1114..1479
                     /gene="mdtJ"
                     /locus_tag="JN691_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAY7245343.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            1466..1795
                     /gene="mdtI"
                     /locus_tag="JN691_004100"
     CDS             1466..1795
                     /gene="mdtI"
                     /locus_tag="JN691_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAY7245344.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1834..2655)
                     /locus_tag="JN691_004101"
     CDS             complement(1834..2655)
                     /locus_tag="JN691_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAY7245345.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(2755..2838)
                     /locus_tag="JN691_004102"
     CDS             complement(2755..2838)
                     /locus_tag="JN691_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245346.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(2931..3266)
                     /locus_tag="JN691_004103"
     CDS             complement(2931..3266)
                     /locus_tag="JN691_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAY7245347.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
BASE COUNT          904 a          831 c          857 g          951 t
ORIGIN      
        1 attaagcgca aaggccagca atccccagac aaaagcgaag cgaacatcga gtgcggcgag
       61 catcgcccag gcgaccaggc cggtgatgat gctgatggct gttttcagca ccagataatg
      121 agaaacactg tcaatcgcac gttgaatcgc cgccatccct tcaaccggac gcgccatcat
      181 ttgctgaaat tttccgggca attgtggcac ttcgagcagc ataaacagca ccgtcagcag
      241 caataaaaat attgatgaca tggcattaga taactgcgtc aataagttgg tgagcaacgt
      301 catcgccgcg ttcggatcaa tgtaatgcgc cagttggtca actgagacgt caatccctac
      361 gcgttgcaac aacggctcaa gagcttgcag cggcgtcata atagagttgc gatattgcgg
      421 taacgtccgc gtcaactcgt tgagcgcgga acccagataa gctaatagca acaccatcgc
      481 catcacgatg attgtcatca aaatcgacac tgccagtaca cgcggcacac gccagcggac
      541 catatgttgc accagcgggt taagaataac agcaataaat aatgcgagaa taaacggcac
      601 gatgatctcg gcggcaaaac ggataccgca gagaataatg accagcattc ccaacataat
      661 gacgattttt aggccattga gcgtgatgat cggctttgcc atgcttgtcc ccgttttttc
      721 ttcctttgct aataataatg tctttttcgc tccgtcatca taacgaaaat caaaccgctc
      781 aggtaaagaa gtgaaaatat tttgagttaa ttcttaagct atgatacaaa tcaggcgtgt
      841 tcaactaccg aggacaatta tcatctgcga tgacgagaag caacactgcg gataattgta
      901 atattatgga caatttgttc aggacgttat tttctacatt tacccatctt cgtacctcat
      961 ccactatctt gctcgtaggt gagcagcact ggcgcaacgc gctttagtcc tttcttctgc
     1021 ctaagcttgc cctatgcgca gggcttgacc actttacttt ggtttcgctg aattaagcga
     1081 aaattaaaat agttctcttg caggagaagg acaatgtata tttattggat tttattaggt
     1141 ctggctattg ctacagaaat taccggtacg ctgtcaatga aatgggcgag cgtcagtgag
     1201 ggaaatggcg gctttatttt aatgctggtg atgatttctc tgtcgtatat atttctctct
     1261 ttcgccgtta aaaaaatcgc cttaggcgta gcttatgcgc tgtgggaagg tatcggtatt
     1321 ttatttatta ccttgtttag cgttttgtta ttcgacgaaa gtttatcgct gatgaaaatt
     1381 gccgggttaa ccaccctggt cgccgggata gtgttgatca aatcaggtac ccgtaaagcg
     1441 cgtaaacctg aactggaggt gaaccatggc gcagtttgaa tgggttcacg ccgcctggct
     1501 ggcattggca atcgtgctgg aaatcgttgc taacgtcttt ttgaaatttt ctgacggctt
     1561 tcgtcgcaaa atatttggct tgctctccct ggcggcggtg ctggcggcct ttagtgcgct
     1621 ttctcaggcc gttaaaggga tcgacttgtc tgtcgcttat gcattgtggg gcgggtttgg
     1681 tattgccgcc acgttagccg caggttggat cttgtttggt caacggttaa atcgtaaagg
     1741 ctggattggc ctggtcttgc tgttggctgg aatgatcatg gtgaaacttg cctgatgaag
     1801 acgctgcccg cgctgtcggg cagcgtttga acattatttt tgcgacagtt gatccagctt
     1861 gtcgcgaaaa ccggtaacag aaatggcccg gttatcggcg cgccagcgat ctttcgcggc
     1921 aggagccgaa ctttgcaccc caattaattg ccagccgtca tcggtatgca acatcagagg
     1981 cgaaccgctg tcacccggca aggtatcgca ctgatgtgac atcaccgacg tttgcgccca
     2041 gccagtcact tcacagtttt gatgactgta taacgtatcg agatgatctt cagggtatcc
     2101 cgcctgagtc actttacgac cagccgattt taatgcggca gtaagcgcgg ctttatctcc
     2161 ctcaaataac ggcaacggtg taatgccaga agggggatta cgtagcacaa tcaatccgaa
     2221 gtcccacggt gcagctgcgg gaggaacaat ccaaccatcc ccatctgctt ttaaccgctt
     2281 tcccagtgtc ggatcaacgc ggccttctat gtcgtggatc tcatagcgcc aaagaccttt
     2341 atttgacaca aaacgcagcg ctaccgcctt atccgcttta cccttaggcg gcgtcaataa
     2401 acagtgtcct gccgttaatg ccagattggg tgcaatcagc gtcgccgtac ataaattgcc
     2461 gctggccgtt tccagttgcc caaccgcatc ccacggtgat tgggtcgtgt cattcactgg
     2521 cacacgatca tcatgaccaa aaaacagggt gctgacctca tcgtttgccg atttggcaac
     2581 gtctggttta tctgcaaaca caaaagcaga tgtcaaacta attgcaccca acactacagc
     2641 aatggttgta cgcatatcac actctggtgg gtaattatgc ttattaaaag caaaccctca
     2701 taaatactat agacgggacg ggatgaaagt gggagtaaaa tcagataact acagtcagga
     2761 aagataaaaa cgggcggcaa tcagtgcgca taaaataagg atgataagaa tgaactcaaa
     2821 gcgataacgg cccaccatac taccctccgc taaaggcggt gctaaacgtt acccgcttag
     2881 caccgtaatt ttcaggcgcg agggggcgcg ccagcattac tgttgaaaac ttacgctgcg
     2941 ggttgtgcag caggttttgc cggttgctga tggccgtgtt tcccggcgtg tttcttcgct
     3001 gcctgcgctt tttgttcagg ggctttctgt tcagctttcg tatttttatg atgcttttta
     3061 gccgcctgcg ctttctgggc aggtgctgct ttatgctgtt ttttatgatg tgtagttttc
     3121 gctggcgctg ctttggtggt cgtcgcagtc ggagcaggtg tggtcgcagt ctctgcagca
     3181 aaggcggcag aagacagacc catagcagcg gcaacaacca gagctaatac ttttttcatt
     3241 gtcataccct caatttgttt tttcatttaa ccccactgcg gggccgttga aataactata
     3301 tccctgggaa tggcagactt ccgtgagtgg ttggtttcag cgtgtaacga tatgtacaaa
     3361 cgctgaataa attacagcgt tgataataac gtattgtgac ttaaggaaaa tttagctacc
     3421 aataatagta gtcttgatcg ggttaactat tatactactt acaaagcggt agaataactg
     3481 cgcatcagta ataaatgaca cacagcaaaa tgaatccgtt tatttgggta cttataatcc
     3541 tga
//
LOCUS       DADLSA010000253         3543 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.256, whole genome shotgun sequence.
ACCESSION   DADLSA010000253 DADLSA010000000
VERSION     DADLSA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.256"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..1031)
                     /gene="curA"
                     /locus_tag="JN691_004104"
     CDS             complement(<1..1031)
                     /gene="curA"
                     /locus_tag="JN691_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HAY7245348.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLAPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGEMLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMVQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAG"
     gene            1212..1730
                     /gene="mddA"
                     /locus_tag="JN691_004105"
     CDS             1212..1730
                     /gene="mddA"
                     /locus_tag="JN691_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAY7245349.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1727..2176
                     /locus_tag="JN691_004106"
     CDS             1727..2176
                     /locus_tag="JN691_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7245350.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2177..2410)
                     /locus_tag="JN691_004107"
     CDS             complement(2177..2410)
                     /locus_tag="JN691_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HAY7245351.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2496..2669)
                     /locus_tag="JN691_004108"
     CDS             complement(2496..2669)
                     /locus_tag="JN691_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAY7245352.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2864..2959
                     /gene="yncL"
                     /locus_tag="JN691_004109"
     CDS             2864..2959
                     /gene="yncL"
                     /locus_tag="JN691_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAY7245353.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(3361..>3543)
                     /locus_tag="JN691_004110"
     CDS             complement(3361..>3543)
                     /locus_tag="JN691_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HAY7245354.1"
                     /translation="VIDELLRIKWWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQ
                     ERDYKKFNLSEVFRGL"
BASE COUNT          875 a          887 c          803 g          978 t
ORIGIN      
        1 tcacccgcga cgcggatcac cactttgccg aagtttttac ccttcagcag gccgataaac
       61 gtctgtggcg cattctctaa accatcagta atatcttcgc ggtagtggat tttatcctct
      121 ttcacccatt gcaccatctc cttctgaaac tcatggatgc ggtgaccata atcctgagcg
      181 ataataaacc cttgcaagcg aatacgtttt ttcagcaccg ttgccatcaa caggggtaaa
      241 cgatccggac cgggtggtag ctctgtagcg ttatagctgc tcactaagcc acaaacggga
      301 atgcgcgcag atgtattaag taatggtagc actgcatcaa ataccttacc gcccacgttt
      361 tcataataga tatcaatacc ttttgggcac gctttcgcca gttgttcggc aaaatcgtcc
      421 gcgtggtgat caaggcaaac atcgaagcct aacacctcgg tagcatggcg gcatttttcc
      481 gcgccaccgg ctacccccac cactctgcaa cctttaagtt tgccgatttg ccccaccgtc
      541 gcccccactg gtcctgtcgc cgcagctacc accaacattt cgccctcttt aggctgaccg
      601 atatccagca gccccatata agcggtaaag cctggcatcc ccagcacacc cagcgaccac
      661 gatggatttt gcggatgatc gccaagtttc accaggtcat caccactgga tatgtcatag
      721 tcctgccatc cactgtagcc cagcacccag tcgccaggct gataatcagg atgattcgac
      781 tccacgacac ggctcaccgt accgccgacc atcacgccgc caatatcaac cggcggtgaa
      841 taagatggct catcgctcat acgtccacgc atatacgggg ccagggacaa ataaactgtg
      901 cgcagtaaca cctgtccttc acccggtgtg gcgacatcat cttcttcaag acggaaattc
      961 tccggaaccg gtgcgccatg tggacgcgag gccagaaccc aacgtcgatt acgctgcttt
     1021 tgttgcccca taatgctctc ctgtgctttt ggcatgaatc catcaatagt agcaggctca
     1081 cgggattggt tttgtttgca gccgtcacat cccctctgca acacgcaata atttcatcga
     1141 ggaatccgtt gcttcgggct gttttcgata gtattgataa catcatcatc attattttat
     1201 tgaggccaga catgtccatc cgttttgccc gcaaagccga ctgtgctgcc attgcggaaa
     1261 tttataacca cgccgtgttg tatacggcgg ctatctggaa tgaccaaacg gtggatgcag
     1321 ataaccgcat tgcctggttt gaagcgcgga ccatagcagg ttatccggtg ctggtaagtg
     1381 aagaagatgg cgtagttacg ggatatgcct cgtttggcga ctggcgtagt ttcgatggtt
     1441 ttcgccatac cgtggaacat tcggtttatg tccatcccga tcatcagggc aaaggtctgg
     1501 ggcgtaaatt gttaagccga ttgattgatg aagcgcggga ttgcgggaag catgtcatgg
     1561 tcgccgggat cgaatcgcaa aatcaggctt cgctgcatct gcaccagtcg ctgggatttg
     1621 tcgtcaccgc acaaatgccg caggtaggca ctaaatttgg tcgttggctg gatctgacat
     1681 ttatgcagtt gcaactcgac gagcgcactg aaccggacgc gattggatga atcagtcgct
     1741 cacccttgct tttttgattg ccgccggaat tggtctggta gtccagaaca cgctgatggt
     1801 gcgcatcacc cagacctcct ctaccattct catcgccatg ttgctgaact cactggtggg
     1861 gattgtgctc tttgtttcta ttttatggtt taaacaaggc atggcggggt ttggcgaact
     1921 ggtgtccagc gtgcgctggt ggacacttat acctggcttg ctgggttcgt tttttgtctt
     1981 cgccagtatc agcggatatc agaatgtggg agcggcgaca actattgcgg tactggtcgc
     2041 cagccagctg attggcggtc tggtgctgga tatcttccgc agtcatggcg taccactacg
     2101 ggcgttgttc ggcccaatat gtggcgcaat tttacttgtg gttggcgcct ggttggtggc
     2161 cagacgctca ttttaatcag aggatggtgc cgcctttggt taactgttcg tggcgcgctt
     2221 ccatatcttc tttatgcttg cgaccgtgat gagcaatagc ggtgcgcaaa cgctgctgct
     2281 gggtgtagcg atcttcccga cttaactccg cgtcatcgct cagggcgatt aataattcgt
     2341 tcatatgggc aatgacgctc tcttcaatcg cggcatccac gcgcgcgatg acttcatcca
     2401 gatgcgacat actttctcct tagccacttg tgaaatttat cgattaatcc aaatgatata
     2461 agtctgcgtg gtgccgtcaa gccagtgagt aagatttaaa acagtgaaaa caacagcgcc
     2521 accgggaaac tcatcggcca tgtggccccc accagaaagg cgcttaagaa acggatgcgt
     2581 ttcttatcgt gagaaagaaa ccaggtgata agaaatgcga ttgctgccat aaccgcataa
     2641 aaaacaagca tatgttgata gagagacatg tacgggcgtc cagccatatg aataaaacgc
     2701 gcgcaaatat gcgtcatttt ttgatgtgca tcaagttttt tcaccagttt gagaaaaaat
     2761 catcaaacag caacttgccc ttatccatct tcagaatgag tattattgcg gattttctta
     2821 acccgtacta tacacatagg ggcaatgata aaaggtggca aaaatgaatg tttccagtag
     2881 aactgtagta ctgataaatg tctttgctgc tgttggtttg tttactctta tctctatgag
     2941 atttggctgg tttatttgat gtagtgattt tgcacgcagc acgtactgtc agagaaatca
     3001 actctctgac agtacgcaag atcttctcat actctctagg tttgcaacat tatccttaat
     3061 atttacttct ttttgctggc atctacattg aaatgagtag aaaacatccc cctactgtcg
     3121 tctatacaaa cagcttactg attcaaacgg aatcttgttt tcacattttt ctatcacgaa
     3181 tactttatac gcgcgggagt ctataaatgc tttatacttt caaggaataa cgtaaatata
     3241 aaaagatggc gtaaagtata tgcatggcag gatgtttgca acctccttga tgacgctgcc
     3301 ccattgaaat tgcaaacccc gccctggtta tcttgcccat ttatcatgca aaacagataa
     3361 ttataggcct ctgaatacct cagaaagatt gaatttttta tagtccctct cttgaatatt
     3421 tccatttgat atcaatcgat tcatctcttc aacaaaataa tttatatcat cacacttgtt
     3481 attatccgga agatctgagt agttataatt ccaccatttt attctcaaca gttcatcaat
     3541 aac
//
LOCUS       DADLSA010000254         3505 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.257, whole genome shotgun sequence.
ACCESSION   DADLSA010000254 DADLSA010000000
VERSION     DADLSA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.257"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..203)
                     /gene="mokC"
                     /locus_tag="JN691_004111"
     CDS             complement(<1..203)
                     /gene="mokC"
                     /locus_tag="JN691_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAY7245355.1"
                     /translation="MLNTCRVPLTDRKVKGKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYES"
     gene            732..1898
                     /gene="nhaA"
                     /locus_tag="JN691_004112"
     CDS             732..1898
                     /gene="nhaA"
                     /locus_tag="JN691_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAY7245356.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1964..2863
                     /gene="nhaR"
                     /locus_tag="JN691_004113"
     CDS             1964..2863
                     /gene="nhaR"
                     /locus_tag="JN691_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAY7245357.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2901..>3505)
                     /locus_tag="JN691_004114"
     CDS             complement(2901..>3505)
                     /locus_tag="JN691_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245358.1"
                     /translation="TIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTNCNS
                     VRFNFNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELV
                     FEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYS
                     FQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          851 a          776 c          844 g         1034 t
ORIGIN      
        1 gattcgtaag ccgtgaaaac agcaacctcc gtctggccag ttcggatgtg aacctcacag
       61 aggtcttttc tcgttaccag cgccgccact acggcggtga tacagatgac gatcagggcg
      121 acaatcatcg ccttatgctg cttcattgct ctcttcccct tgaccttacg gtcagtaaga
      181 ggcactctac atgtgttcag catataggag gcctcgggtt gatggtaaaa tatcactcgg
      241 ggcttttctc tatctgccgt tcagctaatg cctgagacag acagcctcaa gcacccgccg
      301 ctattatatc gctctcttta acccattttg ttttatcgat tctaatcctg aagacgcctc
      361 gcatttttgt ggcgtaattt tttaatgatt taattattta actttaattt atctcttcat
      421 cgcaattatt gacgacaagc tggattattt ttgaaatatt ggcctaacaa gcatcgccga
      481 ctgacaacaa attaattatt acttttccta attaatccct caggaatcct caccttaagc
      541 tatgattatc tgggcttagg gtcactcgtg agcgcttaca gccgtcaaaa acgcatctca
      601 ccgctgatgg cgcaaattct tcaatagctc gtaaaaaacg aattattcct acactataat
      661 ctgattttaa cgatgattcg tgcggggtaa aatagtaaaa acgatctatt cacctgaaag
      721 agaaataaaa agtgaaacat ctgcatcgat tctttagcag tgatgcctcg ggaggcatta
      781 ttcttatcat tgccgctatc ctggcgatga ttatggccaa cagcggcgca accagtggat
      841 ggtatcacga ctttctggag acgccggttc agctccgggt tggttcactc gaaatcaaca
      901 aaaacatgct gttatggata aatgacgcgc tgatggcggt atttttcctg ttagtcggtc
      961 tggaagttaa acgtgaactg atgcaaggat cgctagccag cttacgccag gccgcatttc
     1021 cagttatcgc cgctattggt gggatgattg tgccggcatt actctatctg gcttttaact
     1081 atgccgatcc gattacccgc gaagggtggg cgatcccggc ggctactgac attgcttttg
     1141 cacttggtgt actggcgctg ttgggaagtc gtgttccgtt agcgctgaag atctttttga
     1201 tggctctggc tattatcgac gatcttgggg ccatcattat catcgcattg ttctacacta
     1261 atgacttatc gatggcctct cttggcgtcg cggctgtagc aattgcggta ctcgcggtat
     1321 tgaatctgtg tggtgtacgc cgcacgggcg tctatattct tgttggcgtg gtgttgtgga
     1381 ctgcggtgtt gaaatcgggg gttcacgcaa ctctggcggg ggtaattgtc ggcttcttta
     1441 ttcctttgaa agagaagcat gggcgttctc cagcgaagcg actggagcat gtgttgcacc
     1501 cgtgggtggc gtatctgatt ttgccgctgt ttgcatttgc taatgctggc gtttcactgc
     1561 aaggcgtcac gctggatggc ttgacctcca ttctgccatt ggggatcatc gctggcttgc
     1621 tgattggcaa accgctgggg attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg
     1681 cgcatctgcc tgagggaacg acttatcagc aaattatggt ggtggggatc ctgtgcggta
     1741 tcggttttac tatgtctatc tttattgcca gcctggcctt tggtagcgta gatccagaac
     1801 tgattaactg ggcgaaactc ggtatcctgg tcggttctat ctcttcggcg gtaattggat
     1861 acagctggtt acgcgttcgt ttgcgtccat cagtttgaca ggacggttta ccggggagcc
     1921 ataaacggct cccttttcat tgttatcagg gagagaaatg agcatgtctc atatcaatta
     1981 caaccacttg tattacttct ggcatgtcta taaagaaggt tccgtggttg gcgcagcgga
     2041 ggcgctttat ttaactccac aaaccattac cggacagatt cgagcgctgg aagagcgcct
     2101 gcaaggcaaa ttatttaaac gcaagggacg tggtctcgaa cccagcgagc tgggagaact
     2161 ggtctatcgc tatgccgata aaatgttcac cttaagccag gaaatgctgg atattgtgaa
     2221 ctatcgcaaa gaatccaatt tattgtttga cgttggcgtg gctgatgcac tttccaaacg
     2281 cctggtcagt agcgtactta acgccgcagt ggtagaaggc gagcccattc atcttcgctg
     2341 cttcgaatcc acccacgaaa tgctgctgga gcaattaagt cagcataaac tggatatgat
     2401 catttctgac tgtccgatag actctacgca gcaggaaggc ctgttctccg tgagaattgg
     2461 cgaatgtggc gtgagtttct ggtgtacaaa tccaccacca gaaaaaccgt tcccggcttg
     2521 tctggaagaa cggcgacttt tgattcctgg gcgacgttca atgttagggc gcaaattgct
     2581 taactggttt aactcccagg gattaaacgt agaaatcctc ggcgagtttg atgatgccgc
     2641 tttgatgaaa gcttttggtg cgatgcacaa tgcaatcttc gttgccccaa cgctttatgc
     2701 atatgacttt tatgccgata aaactgtcgt agaaattggt cgcgtcgaga atgtgatgga
     2761 agagtaccat gctatttttg ctgagcggat gattcagcac ccggcggtac agcgaatctg
     2821 caatacggat tattctgcgc tttttagtcc agcggtgcgt taatcggcag ctcccccaaa
     2881 gttaaggtgg gggagataga ttagttgtac attaccacga ttttgactcg gctcattatt
     2941 tgcccgcttg agacattgtt tccatatgta cgcgggcgaa taaatagagg aatctgatta
     3001 cttccttcat ggggatgctg aaaagagtag taattgcttt taccatactg gatttcattc
     3061 gtaccatcat tggagagttg aaagcctatt tgacccgcac cgtcctcact gttttctaac
     3121 tcatttttta atatgctggt tcctgactcc tgtatcgtca ctggttcaaa gtaatattca
     3181 acgtcactgg gttcaaaaac taattcacct tgctcgccct ggatgcagtc aaatcttata
     3241 tgatgaaact gataattttc attattgata cgagacagta caaatcgtcc agtgtcgatg
     3301 gattgatctt caaaaccaca ggtcattaaa tttacctgta aaatagtatt taaattaaaa
     3361 ttaaagcgga cgctattaca attagtgtta ttccctgtac atgaactcac ttcaatttcg
     3421 aacaacaaag gaaaaggcgt tcctggaaaa atatctgcgg tgttggaact atctgataca
     3481 gatgcgatag agtatggaat ggtga
//
LOCUS       DADLSA010000255         3493 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.258, whole genome shotgun sequence.
ACCESSION   DADLSA010000255 DADLSA010000000
VERSION     DADLSA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.258"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..432
                     /locus_tag="JN691_004115"
     CDS             <1..432
                     /locus_tag="JN691_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7245359.1"
                     /translation="PIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGIL
                     QLGVSVMSEFFPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALL
                     ALITAIIVFSRYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            444..1310
                     /gene="ydiB"
                     /locus_tag="JN691_004116"
     CDS             444..1310
                     /gene="ydiB"
                     /locus_tag="JN691_004116"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HAY7245360.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            1341..2099
                     /gene="aroD"
                     /locus_tag="JN691_004117"
     CDS             1341..2099
                     /gene="aroD"
                     /locus_tag="JN691_004117"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAY7245361.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            2245..>3493
                     /locus_tag="JN691_004118"
     CDS             2245..>3493
                     /locus_tag="JN691_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase YdiF"
                     /protein_id="HAY7245362.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLIIALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYAIFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCG"
BASE COUNT          913 a          798 c          874 g          908 t
ORIGIN      
        1 cccatctggg ctaatgtatt taactctgcg ctggcaacaa taacagctgc cattatctac
       61 ctgtaccctt ctccactggt gtgtaatgcc ggcgcctttg ttatcggttt ctcagcagct
      121 ggcggcattt tacagctcgg tgtttcggtc atgtcagagt ttttccccaa aagcaaagcc
      181 aaagtcacca gtatttatat gatgatgggt ggactggcta actttgttat tccgctgatt
      241 accggttatc tgtcgaacat cggcctgcaa tatatcattg ttctcgattt tactttcgcg
      301 ctgctggccc tgattaccgc aattattgtt tttagccgct attaccgcgt ttttattatt
      361 cctgagaatg atgtgcggtt tggcgaacgc aaatttagca cccggttaaa cacaattaag
      421 catagaggtt aaaggagtta attatggatg ttaccgcaaa atacgaattg attggattga
      481 tggcttatcc tatccgccac agtttatcgc ctgaaatgca gaataaagcc ttagaaaaag
      541 cgggattgcc atttacctat atggccttcg aagtggataa cgatagcttt cccggagcaa
      601 ttgaaggatt aaaagccctc aaaatgcgcg gaactggtgt gtcgatgccg aacaaacaac
      661 tggcgtgtga atatgttgat gaattaacgc cagcagccaa actggtgggt gccatcaaca
      721 ccatcgttaa tgatgatggc tatctgcgtg gctataacac cgacggcacg ggccatattc
      781 gcgccattaa agagagcggt tttgatatca aaggcaaaac gatggtgctg ttaggggccg
      841 gaggtgcctc aacggcgatt ggcgcgcagg gggcaattga aggtttaaaa gaaattaaac
      901 tctttaaccg tcgggatgag ttcttcgata aagccctcgc cttcgcgcag cgggttaacg
      961 aaaacaccga ttgtgtcgtc acggttaccg atctcgccga tcagcaagcc tttgctgaag
     1021 ccctggcttc cgccgacatt ttaaccaatg gcacaaaagt aggtatgaaa ccccttgaga
     1081 atgaatcatt ggttaatgat atcagtctgt tacatccggg actgctggtc actgaatgcg
     1141 tgtataaccc gcatatgacg aagttattgc agcaggcaca acaagctggt tgcaaaacga
     1201 ttgatggata cggcatgttg ttgtggcaag gggctgaaca gttcacgtta tggactggca
     1261 aagatttccc tctggaatat gttaaacagg tcatggggtt cggtgcctga caggctgacc
     1321 gcgtgcagaa agggtaaaaa atgaaaaccg taactgtaaa agatctcgtc attggtgcgg
     1381 gcgcacctaa aatcatcgtc tcgctgatgg cgaaagatat cgcccgcgtg aaatccgaag
     1441 ctctcgccta tcgtgaagcg gactttgata ttctggaatg gcgtgtggac cactttgccg
     1501 acctctccaa tgtggagtct gtcatggcgg cggcaaaaat tctccgtgaa accatgccag
     1561 aaaaaccgct gctgtttacc ttccgcagtg ccaaagaagg cggcgagcag gcgatttcca
     1621 ccgaggctta tattgctctc aatcgtgcag ccatcgacag cggcctggtt gatatgatcg
     1681 atctggagtt atttaccggc gatgatcagg tcaaagaaac cgtcgcctac gcccacgcgc
     1741 atgatgtgaa agttgtcatg tccaaccatg acttccataa aacgccggaa gccgaagaaa
     1801 tcattgcccg tctgcgcaaa atgcagtcct tcgacgccga tattcctaag attgcgctga
     1861 tgccgcaaag taccagcgat gtgctgacgt tgcttgccgc gaccctggag atgcaggagc
     1921 agtatgccga tcgtccaatc atcacgatgt cgatggcaaa aactggcgta atttctcgtc
     1981 tggctggtga agtatttggc tcggcggcaa cttttggtgc ggtaaaaaaa gcctctgcgc
     2041 cagggcaaat ctcggtaaat gatttgcgca cggtattaac tattttacat caggcataag
     2101 caataatatt tcggcggaaa taccctcccc cgccgaaata aaaaaagtat ttttaaccgt
     2161 attttataaa aatatttcgt gagtctctgt gcgctaattc tccatctggc gtagggaaaa
     2221 tcacacctga atcaggaatt aacaatgaaa cctgtaaaac cacctcgtat taatggacga
     2281 gtgccggtcc tgtcggcaca ggaagcggtg aattatattc ccgacgaagc aacactttgt
     2341 gtgttaggcg ctggcggcgg tattctggaa gccaccacgt taattattgc tcttgctgat
     2401 aaatataaac agactcaaac gccacgtaat ttatcgatta ttagtccaac agggcttggc
     2461 gatcgcgccg accgcggtat tagtccgctg gcgcaagaag gtctggtgaa atgggcatta
     2521 tgcggtcact ggggacaatc gccgcgtatt tctgatctcg cagaacaaaa taaaattatt
     2581 gcttataact atccacaagg tgtacttaca caaaccttac gcgccgccgc agcccaccag
     2641 cctggtatta ttagtgatat tggcatcggg acatttgtcg atccacgcca gcaaggcggc
     2701 aaactgaatg aagtcactaa agaagacctg attaaactgg tcgagtttga taacaaagaa
     2761 tatctctatt acaaagcgat tgcgccagat atcgccttca ttcgcgctac cacctgcgac
     2821 agcgaaggct acgccatttt tgaagatgag gtgatgtatc tcgacgcatt ggttattgcc
     2881 caggcagtgc acaataacgg cggtattgtg atgatgcagg tgcagaaaat ggttaagaaa
     2941 gccacgctgc atcctaaatc tgtccgtatt ccgggttatc tggtggatat tgtggtggtc
     3001 gatccggatc aaacccaact gtatggcggt gcaccggtta accgctttat ttctggtgac
     3061 ttcacccttg atgacagtac caaacttagc ctgcccctaa accaacgtaa attagttgcg
     3121 cggcgcgcat tattcgaaat gcgtaaaggc gcggtgggga atgtcggcgt cggtattgct
     3181 gacggcattg gcctggtcgc tcgggaagaa ggttgtgctg atgactttat tctgacggta
     3241 gaaacaggtc cgattggcgg aattacttca cagggtatcg cctttggcgc gaacgtgaat
     3301 acccgcgcca ttctggatat gacgtcccag tttgattttt atcacggtgg tggtctggat
     3361 gtttgttatt tgagttttgc tgaagtcgac cagcacggta acgtcggcgt gcataaattc
     3421 aatggcaaaa tcatgggtac cggtggattt attgatatca gtgccacctc gaagaaaatt
     3481 attttctgcg gca
//
LOCUS       DADLSA010000256         3464 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.259, whole genome shotgun sequence.
ACCESSION   DADLSA010000256 DADLSA010000000
VERSION     DADLSA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.259"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            1..1981
                     /locus_tag="JN691_004119"
                     /pseudo
     CDS             1..1981
                     /locus_tag="JN691_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
     gene            1987..2700
                     /gene="ybiX"
                     /locus_tag="JN691_004120"
     CDS             1987..2700
                     /gene="ybiX"
                     /locus_tag="JN691_004120"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAY7245363.1"
                     /translation="MWGAGPTFWRTCMMYHIPGVLSPQDVAHFREQLEQAEWVDGRVT
                     TGAQGAQVKNNQQVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNN
                     ETYGFHVDGAVRSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGD
                     LVLYPSSSLHCVTPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENE
                     EILSLLNLYHNLLREWSEI"
     gene            2774..3040
                     /gene="ybiI"
                     /locus_tag="JN691_004121"
     CDS             2774..3040
                     /gene="ybiI"
                     /locus_tag="JN691_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HAY7245364.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            3305..>3464
                     /locus_tag="JN691_004122"
     CDS             3305..>3464
                     /locus_tag="JN691_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245365.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEA"
BASE COUNT          893 a          922 c          869 g          780 t
ORIGIN      
        1 gggtgcgttt tttgcgggtg agaacggtaa ctccaccact ggcgacgcca tttatatgcg
       61 tggtgccgat acctctaaca gtatttatat tgatggtatt cgcgatatcg gcagcgtctc
      121 gcgcgacacc ttcaataccg agcaggtcga agtgattaaa gggccgtccg gcaccgacta
      181 cgggcgcagc gcaccgacag gctcgatcaa tatgatcagc aagcagccgc gcaatgattc
      241 cggcattgac gcctccgcca gtattggcag cgcctggttc cgccgcggca cgctggacgt
      301 caatcaggtc attggtgata ctaccgcggt gcgcctgaat gtgatgggcg aaaaaaccca
      361 cgatgccgga cgcgacaaag tcaaaaatga gcgttacggc gtcgcccctt ctgtcgcttt
      421 tggccttggt acagcgaatc gtttgtatct taattatctg catgtcactc agcacaacac
      481 gccagacggc ggcattccga ccatcggttt gccgggctat tctgccccat ctgcgggagc
      541 ggcggccctg aatcattccg gaaaagttga tactcataac ttttacggca cggattccga
      601 ttacgacgat tcgaccaccg acaccgccac catgcgtttt gagcacgaca tcaacgataa
      661 caccaccatt cgcaatacta cccgttggtc gcgcgtaaag caggattacc tgatgacggc
      721 gattatgggc ggggcgtcga atattactca gcccaccagc gatgtgaata gctggaccta
      781 gtcacgcacg gcgaatacca aagatgtgag taataaaatt ctcaccaacc agaccaacct
      841 gacctcgacg ttctataccg gttctatcgg tcatgatgtc agtaccggcg tggaatttac
      901 ccgtgaaacg cagactaact atggcgttaa tccggtgacg ttaccggcgg taaatattta
      961 tcatcctgac agcagaattc atcccggcgg cctgacgcgc aacggcgcaa acgccaatgg
     1021 tcagacggat accttcgcaa tttacgcctt cgatacgctg caaatcaccc gtgattttga
     1081 gctgaacggc gggatccgtc tggataatta tcatactgaa tatgacagtg ccaccgcctg
     1141 cggcggcagc ggacgcggtg ccatcacctg cccagctggt gtggcaaaag gttctccagt
     1201 caccaccgtc gacaccgcca agtcgggcaa tctggtgaac tggaaagccg gggcgctgta
     1261 tcacctgacg gaaaacggca atgtctatat taactatgcc gtttcccagc agcctccggg
     1321 cggcaacaac ttcgcccttg cgcagtctgg cagtggtaac agtgccaacc gcaccgattt
     1381 taaaccgcaa aaagccaaca ccagcgagat tggcaccaaa tggcaggttc tggataaacg
     1441 cctgttgctc accgccgcgc tgttccgtac tgatatcgaa aatgaagttg agcaaaatga
     1501 tgacgggact tactcgcaat acggtaagaa acgcgtcgaa ggctatgaga tatccgtggc
     1561 agggaatatc actcccgcgt ggcaggtgat tggcggctat acccagcaaa aagcaaccat
     1621 caaaaacggc aaagatgttg cccaggatgg ttcctcatcg ctgccgtata ccccggagca
     1681 cgccttcacc ttatggagcc aatatcaggc aaccgatgat atctctgttg gcgcgggcgc
     1741 acgctatatc ggcagtatac ataaaggttc agacggcgcg gtgggaacgc cagcatttac
     1801 agaaggttac tgggtcgccg atgccaaact ggggtatcgg gttaatcgca atctcgactt
     1861 ccagctaaac gtttacaacc tgtttgatac cgattacgtc gcctcaatca acaagagcgg
     1921 ctaccgttat cacccgggcg agccaagaac cttcttgctc acagccaata tgcatttctg
     1981 attcagatgt ggggcgcagg ccccactttt tggagaactt gtatgatgta ccacattccc
     2041 ggcgtgttat cgccacagga cgtcgcccat tttcgcgaac aactggaaca agccgaatgg
     2101 gtggatggac gcgtcaccac cggagcacaa ggtgcacaag ttaagaacaa tcagcaagta
     2161 gacacccgca gcgcgttata cgccgcactg caaaatgagg tgctgaacgc ggttaaccaa
     2221 catgcattgt tctttgccgc tgccttgccg cgtacccttt ccacgccgct gtttaatcgc
     2281 tatcagaaca atgaaaccta tggtttccat gtggatggcg cagtacgcag ccatccacaa
     2341 aacggctgga tgcgcactga cctttctgcc acgctgtttt taagcgatcc acaaagctac
     2401 gacggcggcg aactggtcgt taatgacacc tttggacaac atcgggtaaa actcccggca
     2461 ggtgatctgg tgttgtatcc ctccagcagc ctgcattgcg tgacacccgt aacccgcggc
     2521 gtacgagtgg catcatttat gtggatccag tcgatgatcc gcgatgataa aaagcgcgcc
     2581 atgctgtttg aactggacaa aaatattcag tcgctgaaaa gccgctatgg tgaaaatgaa
     2641 gagatcctgt cgctgctcaa tctttatcat aatctgctgc gggaatggtc ggagatctga
     2701 cgcttgaact gcccaggttc gtccctatat tagctacact taactgtaca agcattgata
     2761 tggggaggac gatatggcat ccgcttgggc taacgatgac gccgtcaacg aacagatcaa
     2821 cagtacaatt gaagatgcga ttgcccgcgc tcggggtgaa attccgcgtg gcgaaagcct
     2881 gtatgaatgt gaagagtgcg gtgcccccat cccgcaagcc cgtcgggaag ccattcctgg
     2941 cgtgcgctta tgtattcatt gtcagcagga gaaagattta caaaaaccag cgtatacagg
     3001 atataatcgc agaggttcga aagacagcca gttacgttaa ctacaactgg cgagatgcat
     3061 agcgagtata atttgtattt tgcgtggcat tccgtgcctt taacgctgct gtgactgcca
     3121 cgcggcataa aacaagtcca taagtacaac aaataaatgg tttatcagta tgttagatat
     3181 caatcaattt atttgaacaa ggcggtcaat tctcttcgat tttatctctc gtaaaaaaac
     3241 gtgatactca tcacatcgac gaaacaacgt cacttataca aaaatcacct gcgagagatt
     3301 aattatgaaa actatcaata ctgttgttgc tgctatggct ctttcaactc tgtcatttgg
     3361 cgtgttcgcg gcggaaccgg taacggcatc ccaggcacag aacatgaata aaatcggcgt
     3421 ggtttctgcc gatggcgcat ccaccctcga tgccctggaa gcga
//
LOCUS       DADLSA010000257         3460 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.260, whole genome shotgun sequence.
ACCESSION   DADLSA010000257 DADLSA010000000
VERSION     DADLSA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.260"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..987)
                     /locus_tag="JN691_004123"
     CDS             complement(<1..987)
                     /locus_tag="JN691_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HAY7245366.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLL"
     gene            complement(1044..2405)
                     /gene="allB"
                     /locus_tag="JN691_004124"
     CDS             complement(1044..2405)
                     /gene="allB"
                     /locus_tag="JN691_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HAY7245367.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHICHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(2465..>3460)
                     /locus_tag="JN691_004125"
     CDS             complement(2465..>3460)
                     /locus_tag="JN691_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoin permease"
                     /protein_id="HAY7245368.1"
                     /translation="LSLPGLITFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGG
                     MAIWAISLVGLGPIFDYIPSGIQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAR
                     SFREQALGQTLGLVVAYILFAVAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFA
                     VLVILMTTISTNATGNIIPAGYQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQD
                     SIYLFLDIIGGMLGPVIGVMMAHYFVVMRGKINLDELYTAPGDYKYYDNGFNLTAFSV
                     TLVAVILSLGGKFIPFMEPLSRVSWFVGVIVAFVAYALLKKRTTAEKTGEQKTIG"
BASE COUNT          982 a          927 c          803 g          748 t
ORIGIN      
        1 cagcaggcaa ataacgctgc cataaataaa cgaacgccgc gtgtaatcgc cagtctgtgt
       61 taataaacca atggatgaaa caaacggtga aaatggaatt accgccagcg gtacggttat
      121 cagcgtcata aatccggtcg ccacaaagct acgacgataa cgcgtattcc ccgcgccctg
      181 ctgcggataa aaaacatccg tgccccgaat cgcaccgtag gtattgctga tattcaccag
      241 acctgtaatc accgccgtca gaataattcc cggcgacaaa gcaccgccac tgccgagcgg
      301 gaaccactgc cagtgcaact caccggagag cgaatgcgaa gaaggaaagc aaaagtacca
      361 caacaaccag ccggttatcg taccgaccag caggccataa cgggcaaaac gttgcggcag
      421 aaagataatc atcgccagta ccaggcacat caccgccacc gagagcgcga acggtggtaa
      481 ctgaatttta aaatttgggt cggctatgcc aaacggcagt ccgagcatac ctttgaaaaa
      541 gatagtggtc agctgcgcgc ccagcatcaa cataaacaag accatcaccg acggcgtaaa
      601 taaccgtgcc aggcgatggc ctaatccgct aaaaccaatc aacatcgtca gcacaccgga
      661 gagtgcaatc cctaccgcca gactggtagc gatatcgttg atcggtgtcc cgcgcgatgc
      721 ttcgccaagg gtgatagtaa ggatggttcc ccaccacagg ccacctggcc cttccataat
      781 agcgcgacga tgaccgcaaa acgcctgagc gaagcaggcc agtgcggtag caagaaaagc
      841 atattgcgtg agcgtaagca ggctactttg cggcaactga aaagcagaaa gtagcgtagg
      901 aggaaccaca accgtgttgc aaaaaataaa gaaaaaccac tgaaatcctg ataacaggct
      961 ttcgcggctg actgcaaaat tgaacataaa cacaccttaa ccggagaatg cccgccgcaa
     1021 ggacgggcag tgcaggggcc agattactgc tgatgtttaa ggataaattg acctttcggc
     1081 gcaacaggga agccctgttc aatgtcgtaa atcacatcac cacgtaagat ggttttcgtg
     1141 atacgcgcgc caatggtacg gccaacatac gggctgactt tgtggcgata ttccagatcg
     1201 tcattggtaa gaacatagct gctattcggc tgaatgaaga cgaagtcggc atcttttcct
     1261 ggggcgatac ggcctttttg ctgcagaccg aaaatatctg ctgcgttagt cgccattaat
     1321 ttgccgaaca ttggcagaga cattccgcgt ttctgtaccg cttcatcgaa catcacgtcc
     1381 atacagtttt gcagaccagc aataccgccc catgcttcca tgatgttgcc ggctttcatt
     1441 tccggagggc atggtgagtg gtcggaaacc aggcagtcga tttcaccgtt aaacagtttt
     1501 tcccacatgc ctttctggtt ttccagatcg cggatcggcg gtgaacactt cgccagagta
     1561 ccgatttctt cgaactgatc ggtatccagt acaaagtaat gcgggcagga ttcacaggta
     1621 acatcctgac cttcctgacg tgcacgagtc acttcttcaa caccttccgg gctgctgata
     1681 tggcaaatgt gcagacggca accggcaact ttcgccaggt acagtacgcg gcgaatcgct
     1741 tccacttcgg taaataccgg acgcgaagcc acatagtcat gggcagttac gcgaccttca
     1801 cgtttcgctt cttcgcccag tgcatcacag atcagcgcgt tttcgcagtg caccagcacc
     1861 ggctgcccca gttcgcccag cttctgcgca cctttgaaga actgccagtc gtttacatca
     1921 cggaagtcgt tgtcgatacc gcgatcgcca caggtcgcaa cgaagcattt gaagccgaca
     1981 acgcccactt catccaactc atgcagacga tcaatgttgt aagacaccag gccaccgagt
     2041 tgtgccgcat cgatagtcag cttgccttta gcggcatcga acttcagttc aattgaagca
     2101 cggtcaaccg ttgcaggcag ctggttgagc ggcatttcga tcatggtggt gataccacct
     2161 tttgccgctg cgcgagtacc ggtttcataa ccttcccagt ggctacgacc cggttcagaa
     2221 atatgggtgt gcgcatcaac catgcccggc gaaaccacca gaccagacgc atccataact
     2281 tcttttgcat cgcccagatc ctgaccgata gcagcaattt ttccgccttt aacggcgata
     2341 tctacaacgc gagcttcgtt ttctaaaata acggtgccgt ttttaatgat taaatcaaaa
     2401 gacataacaa actccttgta aaatacagat tggtttttaa ctcaatgttc aatatcggga
     2461 ttaattaacc tatggttttt tgctctcctg ttttttctgc tgttgtacgt ttctttaata
     2521 aggcgtaggc cacaaaggcg acgatgacgc cgacaaacca tgaaacacgc gataacggtt
     2581 ccataaaggg aataaactta ccgccaagag ataaaataac ggccaccaga gttactgaaa
     2641 acgcagtgag gttaaaaccg ttatcgtaat atttataatc gccaggtgcg gtatacagtt
     2701 catcaagatt aattttccca cgcatcacca caaaataatg cgccatcatg acaccaatta
     2761 ccggaccaag cattccgccg ataatatcga ggaacagata aatactgtcc tgattttcca
     2821 ttaatttcca cgggcagatc agcaggctga taatactggc aatcagtacg ccgtttttat
     2881 aggtcagttt cgtcggtgca atagcggcaa tctgataacc ggcaggaata atattaccgg
     2941 tggcgttagt ggagatagtc gtcatcagaa taaccagtac cgcaaagaac gaggcgaaca
     3001 agctgtccca acgctgaaca atatccagca cgttccaggt atcagcgccg taatgaatac
     3061 tggctccggc aataatacat acccccgcga ccgcaaacag aatataggcc acaactaaac
     3121 ccagcgtttg ccccagcgct tgctcacgaa acgagcgggc gttttgcgta aagtcggatg
     3181 cgctcaccgc cggtgccgcc cagaccgcaa ctaccgcgtt tatcaccacc aggaacagga
     3241 agccactgtt ttctgctttc tgaataccgc tcggaatgta gtcaaagatt ggaccgagcc
     3301 cgaccagcga aatcgcccaa atcgccatac cgccgaaaac gatatagatg cacgggttaa
     3361 gaatggcagt gaatttattt aaaactttgc caccgccaaa acctatacca acgttgacca
     3421 gccagaagag taagaaagta attaagcccg gtagagaaag
//
LOCUS       DADLSA010000258         3420 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.261, whole genome shotgun sequence.
ACCESSION   DADLSA010000258 DADLSA010000000
VERSION     DADLSA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.261"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..157)
                     /locus_tag="JN691_004126"
     CDS             complement(<1..157)
                     /locus_tag="JN691_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY7245369.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKKGN
                     MFNQLLAS"
     gene            complement(154..444)
                     /locus_tag="JN691_004127"
     CDS             complement(154..444)
                     /locus_tag="JN691_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY7245370.1"
                     /translation="MVDLRKAARGQMCTVRIPDYCNHDPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(444..1043)
                     /locus_tag="JN691_004128"
     CDS             complement(444..1043)
                     /locus_tag="JN691_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY7245371.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCEFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPSEQKLVSGFVEYLYESVGREHTPALSDAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(1110..1370)
                     /locus_tag="JN691_004129"
     CDS             complement(1110..1370)
                     /locus_tag="JN691_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245372.1"
                     /translation="MKIEELREIFSEDGLYTVCVENDAIVSHCCIKCLQFQQRKSGVA
                     LIYFADGLVTDGFLLRGDEFVTSLRALKETGLKAGFSAFECE"
     gene            complement(1594..1806)
                     /locus_tag="JN691_004130"
     CDS             complement(1594..1806)
                     /locus_tag="JN691_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7245373.1"
                     /translation="MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIATALVT
                     RKDLCEVRIRTGQTEVAVFVDYESRE"
     gene            complement(2022..2567)
                     /locus_tag="JN691_004131"
     CDS             complement(2022..2567)
                     /locus_tag="JN691_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY7245374.1"
                     /translation="MNSVSVENLPSLTHQHLPVITTELLAELYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDELRELKNRNSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDSYFNQYEKERATGKMNIDIHLAIRDDKTAFIRSYAPGQLVTVEEALML
                     LQSRGWLVMPRDELAGKLMEL"
     gene            3101..3388
                     /locus_tag="JN691_004132"
     CDS             3101..3388
                     /locus_tag="JN691_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HAY7245375.1"
                     /translation="MAQVYFDTLKFVETLEAAGMPAAQARAISAAVKDSHEAAEVATK
                     RDLNDVRTELKNDIAALDAKIDKLSLQLTVRFGGMLVVAVGALTAIIKLPL"
BASE COUNT          831 a          902 c          774 g          913 t
ORIGIN      
        1 tggatgccag caactggtta aacatatttc ccttcttacc gccgccaata tacgaccagc
       61 gcccccacat gcgcagtttt ccctgaatcc agacactttc cagcgtggtg agacgaaggt
      121 gttccccgct ttttcctgta ttcgttgggt aaatcacaaa tatccctcct ttctccagat
      181 ttcttgcgtg cgaaaaacac cttctgcatg catcaggcgt aattcttctt tggtgtaatc
      241 gcttgttttt acccgcccgt cgattaaatc gtggcatgag ctacaggcaa ttgctgcctg
      301 catatcgtgt ggttttgtcg ctgttccgca cgtccccgcc agcctgtaat gcgccagcac
      361 agaagtttcg ggatcgtgat tgcagtagtc aggaattctg acggtgcaca tctgcccccg
      421 cgccgcttta cgtaaatcca ccattacgca aactccagta gctgcgcggc cacattttcg
      481 acttgttccg gagaggaaaa tttacggaac aggatccagt tccacagcac attcagcaca
      541 gatttataaa cctgctgaaa ctcggtttcg tccatgttcg caaatgcgat agattttgcc
      601 ctgcgcccac gactaccatc aggataaata tgctcggtgt aaaatccggc ctgaatggtt
      661 acccactcgc ggaaagcctc aaacgacttt agcaatgccg tatcccgggt tctgcgtgtc
      721 gcaacggtat tcagatattg ctctgcggca tcactcaagg ctggagtgtg ttccctacct
      781 actgattcgt acaggtactc aacgaagcct gaaaccagtt tttgttcgct aggcgtgatc
      841 gcccctccga ccggagtcca gtaatcgaac cccagttgca ggagtttgaa aaaacgcttg
      901 tggaatgcgt agttacgaac gcgcttaaaa tcagcgtgta tccactcgcc tgttttgatt
      961 tgatgcagaa attcgcaact ctccggcgtc gccgggagaa gtaatccgga agaggtttgt
     1021 ttgaccagtt gtatatgtgc catcggcttt ctccggtggc acggtgttac tcagcagggg
     1081 ggcaaccctg cactgaattg tagatgaact cactcacatt caaaagcaga aaagccagcc
     1141 ttaagtccag tctctttcag cgcccgtaat gacgtgacaa attcatctcc acgcaaaaga
     1201 aaaccgtccg tcacaagtcc gtccgcgaaa taaattaacg ctactccact ctttctttgt
     1261 tgaaattgta aacatttaat gcagcagtga ctgacaatag cgtcattctc aacgcacaca
     1321 gtatagaggc catcttcact aaaaatttca cgtaattctt cgattttcat caacagaatc
     1381 cttccagata aatagctctc ccctgttcgg ggtccacccc tcttctccct gcgcgctact
     1441 taagtgtttt tgattctatt cctgcgcatt ctgaaagttc aaacgcgttg aaaatcgcaa
     1501 caaaaaaccc gccgaagcgg gttaagtgcg ggtgcgttga ggatgcctga cacatcagag
     1561 gtggcgaggg atttctccct cgcctggtca ctcttactct ctagattcgt agtctacgaa
     1621 gacagcgacc tccgtctggc cggttcggat tcgtacctca cagaggtctt tcctcgttac
     1681 cagtgccgtc gctatgacgg ttaaacagat gacgatcagg gcgattaaca tcgccttttg
     1741 ctgcttcata gcctgcttct ccttgccttt cggcacgtaa gaggctaacc tacatgtgtt
     1801 cagcatggat tgagcctcag attaatgtta agcgtcttgc aggacgcgta atgttaactg
     1861 gggcttttct ctatctgcct tttggtgttc atgcctgagg cagatagcct caggcacccg
     1921 cagcaattct acttgacttc cctttcaccg caaatcattt tatcctcggt ggtaatgttc
     1981 tcccgatatg ggaattccca tatcggggct aatttaacca gttaaagctc cattaatttt
     2041 ccggcaagtt catctcgcgg cattaccagc catccgcgcg attgaagcaa catcagggct
     2101 tcttcaaccg tcaccagctg accaggcgca taacttcgga tgaaggcagt tttatcatca
     2161 cggatcgcca ggtgaatatc gatgttcatt ttccccgtag cccgctcttt ctcgtactgg
     2221 ttgaagtagc tgtcttccag tttttcgaat acttcccacg cctgatcggt ttcgagcatt
     2281 ttggcgtgac gggctgcacc tcgttctgtc cagaggatga gtgagcgaac attgcgggcg
     2341 attttcacag aaactcttaa agagtttctg ttcttcagtt cccgtaattc atcgccttcg
     2401 atcaggaaaa agtgttttcc ctctataaac cgctctttgt ttctattgaa attgtttgtc
     2461 aaacgctggc gctctgttcc gtacagctca gccagaagtt ctgttgttat aacaggaaga
     2521 tgttgatgtg taagtgatgg gaggttttca acagatacgc tgttcatgaa tatgtcctac
     2581 tgaatatttt gatttacccc ttttgagagg gtgaccgggc gctcaaaacc gtcagtagtc
     2641 ggcgggcgta ttcccattgc tggtattgta ttagccgcac gcccagtcat aaaccaagaa
     2701 ttctggacac aaaaaaaacc gcatgtctgt cgggcgcggt aaccgctact gaaaggtgtt
     2761 ttgagcaccg tcgaggaatg taatctgcta acatgaaact gtcaatacat tcatgttcca
     2821 atcatcgggt ggcgaactgt gcggggttag cactaccggg tacggaaacc ggcgagcctt
     2881 tcggctcccc cacacagccc gccataaatc gcgaatgtga ctatgcaaac gatatgaaaa
     2941 aagacgcggg cgcgtctcat atcgctccgt aaacatccgg ggtgctaatc ccgacgccag
     3001 attttgctgg cgcgtgagga atatagcccc ggattaacgt tgtcgtcaac agccactaca
     3061 ggctaaacta acaatattcc ccaacaacga aggatttaac atggctcagg tctattttga
     3121 tacactgaag ttcgtcgaaa ccctggaagc cgcaggaatg ccggcagctc aggcgcgcgc
     3181 tatatctgcc gcagtgaaag attctcacga agccgcagaa gtcgccacaa agcgcgacct
     3241 gaatgatgtc agaactgaac taaaaaacga cattgcagcc cttgatgcaa aaatagacaa
     3301 actcagcctg caactgaccg tcagatttgg tggcatgctc gtcgttgctg ttggcgcact
     3361 gacagccatc atcaaattac ctctttaaat taccagcccg ttcggggctg gtttacctta
//
LOCUS       DADLSA010000259         3322 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.262, whole genome shotgun sequence.
ACCESSION   DADLSA010000259 DADLSA010000000
VERSION     DADLSA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.262"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            1..415
                     /locus_tag="JN691_004133"
                     /pseudo
     CDS             1..415
                     /locus_tag="JN691_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847259.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1000..1716
                     /locus_tag="JN691_004134"
     CDS             1000..1716
                     /locus_tag="JN691_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245376.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGSYEINDVYVRK"
     gene            2268..2672
                     /locus_tag="JN691_004135"
     CDS             2268..2672
                     /locus_tag="JN691_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245377.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2735..3034
                     /locus_tag="JN691_004136"
     CDS             2735..3034
                     /locus_tag="JN691_004136"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7245378.1"
                     /translation="MTKTVSTSKKTRKQHSPEFCSEALKLAERIGVAAAARELSLYES
                     QLYAWRSKLQQQMTSSERESELAAENARLKRQLAEQAEELAILQKAATYFAKRLK"
     gene            3031..>3322
                     /locus_tag="JN691_004137"
     CDS             3031..>3322
                     /locus_tag="JN691_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7245379.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTGISPRQQ
                     FRQHCDSVVLAAAFTRSKQRYGAPRLTDELRAQGYHFNVKTVAASLRRQGLRA"
BASE COUNT          932 a          683 c          772 g          935 t
ORIGIN      
        1 cggggaagac ccagatatga acaggcacat tcagagttac tttaaagagc ttactgaacg
       61 tctgaggtgt tagagccatg actgccgcag cattgctggc ggaaatgcct gaatcgagca
      121 gcctctcgag acgtgaaatc agcgcacttg tcggtgttgc tcaggtaaac cgggactcgg
      181 gaactctgcg cggtcgacga accattttcg gtgattgtgc tggggaagaa cagctctgta
      241 catggcggcg cttgaggcac cccggtttaa tctggtgata aaggcgtttt acatgcgcct
      301 acttgcagcc ggaaacgcca aaaaagtggc actggttgcc tgtatgcgta agttgctgac
      361 cattatgaac gcaatgctca gaaaaaacga agaatggaat gaatcgtatt tatgaaaact
      421 ggattgtggg ccataaatat tccaaaaaaa tagcccgctc cgtttattac cggaacgggc
      481 gagagcatga cattgtctgt aattataatg agactgctga ttctacttat ttttaatttc
      541 aagaaaagcg ttttctacaa agaattatga gatttaatgt tttgtttaga caatattatc
      601 attctgttca ataatgaaag tcttcatcaa caacaagtaa aaacaacgtc cctctcatcg
      661 aaccggactt gcagatttcc cgcatccggc tctcttgcaa cacatcagac cttcacgttc
      721 gggcggttat ggggaaggtc cagcaagctg tatgagccct aactgtttga ataccagtag
      781 attaccagaa acttctatcg attgaagaca ttaccaacta gcgttactaa atgttgacca
      841 agttttgttc acttgtctgg ttttgatagt agcattctgc agagtatttt gatatgataa
      901 atatgcatta acaatgatat tattgttaat ttcctagcat gaaaattaaa agatcaactt
      961 tttatctcga atatttttta cattatcagt tggtttctta tgaatgacaa ttcattgctt
     1021 agaaattctt cattattcat cgcctatatg ggatgtgtag ggtgggtcag tgcttattct
     1081 tatggctggg gaacctcttt ttactatgga tttccatggt gggttgttgg tgcaggtttg
     1141 gatgatgttg ccagaagttt actctatgct atcatagtaa tgggtatact ttttaccggt
     1201 tggggcattg gtattttatt ttttcttctt ataaagaaaa gaagtaaaat acaagacttg
     1261 agttttttta gactattttt tgctattacc ctactgtttt tccctgtaat attcgaactt
     1321 ttaattctca aacaatattt catacttcca ttatcacttt catgcattat tagttcattg
     1381 gttatttcaa taataattcg tatatatgga cggatctttt ctgtatcatg tttttcagat
     1441 atcccttttg ttagagaaca ccgcattaag ttaattatgg ctggtttttt ggtttatttt
     1501 tggctttttt cctttcttgt tggctggtat aaacctcaat taaaaaaaga atatcagatg
     1561 ctttgttata ataatagttg gtattatgtt cttgctcgtt atgattcacg gctcgttttg
     1621 tctagttcat ttaaagatga cagtaacaga ttcttaatat ttaatacaga acagtcggga
     1681 tcttatgaaa tcaatgatgt ttatgtcaga aagtaataat cctctcccac aaagtgaatt
     1741 aaatgaatat ataatgcgtt cgaagtatat gtattgagct gtcatactga gaaacaaacc
     1801 ttagggcagc acaattaaag ccagaaacaa ctatgcaaga aagttactaa ataagtgctc
     1861 ggaacagtaa aacactcagg ggggactcag tccagattat gtaatcagat aaattgccaa
     1921 acatcacaaa tcaccagccg gactgagtga tgccgagcat tgcaccaata ccccgtgatg
     1981 aacgatgcct gatgcagaaa gctccctata aaacaaagaa caaaaactac gaccggcgat
     2041 tcaccgtcat actgatgctg catcgtggtg acactgtctg tcactttcat gttgactgaa
     2101 caactctgct gcgaagaagg tgtaccgccg gcgggatttg attgtccggc gtctagcttc
     2161 cacttccctg atggaactga aagttgttat ggccggtcag cttgccctgt ccgcaggaca
     2221 tgaggtgctg tgacggacag caccagtaac ggaaagtgac aatgaaaatg aatactgcgc
     2281 tgatagttgc actaatgtgt atgtggtatg cggtacccgc tgctgcaaaa gagactttac
     2341 tggctatgcc acgtaattca acagaacatt gctatgcgga gattaatgtg cacggaccat
     2401 atggggtgta tttccgagtg gtcccgcatc cccccggggg aaagagctgg gtcgaatgta
     2461 acagtgacta ctattactct gataaaccac cgggagtaca gatactcgga acccgtgcag
     2521 ggtgtcgggt atacggaatc tgcgggacca cctcgacact gcatgtggct ggaagagggg
     2581 ttgtgtgtat taaaaatata tgcagtccac gtgggatgat aattcacagg atacggaaac
     2641 gtcctgtagt ggctgtaagc gatgagatgt gatcttaccc agcaatagtg gacacgcggc
     2701 taagtgagta aactctcagt cagaggtgac tcacatgaca aaaacagtat caaccagcaa
     2761 aaaaacacgt aaacagcatt cacctgaatt ttgcagtgaa gccctgaagc ttgctgaacg
     2821 catcggtgtt gctgccgcag cccgtgaact cagcctgtat gaatctcagc tctatgcgtg
     2881 gcgcagcaaa ctgcagcaac agatgacctc gtctgagcgt gaaagtgaac tggccgctga
     2941 aaatgcccgc ctgaagcggc aactggcgga gcaggcagag gaactggcta tcctccaaaa
     3001 ggccgcgaca tactttgcga agcgcctgaa atgaagtatg tctttattga aaaacatcag
     3061 gctgagttca gcatcaaggc aatgtgccgc gtgctccggg tggcccgcag cggctggtat
     3121 acgtggtgtc agcggcggac agggataagc cctcgtcagc agttccgtca gcactgcgac
     3181 agcgttgtcc tcgcggcggc ctttacccgg tcaaaacagc gttacggtgc accacgcctg
     3241 acggatgaac tgcgtgctca gggttaccac tttaacgtaa aaaccgtggc ggcaagcctg
     3301 cgccgtcagg ggctgagggc aa
//
LOCUS       DADLSA010000260         3320 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.263, whole genome shotgun sequence.
ACCESSION   DADLSA010000260 DADLSA010000000
VERSION     DADLSA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.263"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..859)
                     /locus_tag="JN691_004138"
     CDS             complement(<1..859)
                     /locus_tag="JN691_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY7245380.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGQVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQCFFRDHNI
                     TIEMTESDAE"
     gene            complement(878..2755)
                     /gene="bglF"
                     /locus_tag="JN691_004139"
     CDS             complement(878..2755)
                     /gene="bglF"
                     /locus_tag="JN691_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HAY7245381.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2889..>3320)
                     /gene="bglG"
                     /locus_tag="JN691_004140"
     CDS             complement(2889..>3320)
                     /gene="bglG"
                     /locus_tag="JN691_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HAY7245382.1"
                     /translation="EALTIIDKRLGVQLPKDEVGFIAMHLVSAQMSGNMEDVAGVTQL
                     MREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASINDSDESLQQAVKQNY
                     PQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
BASE COUNT          871 a          898 c          752 g          799 t
ORIGIN      
        1 cttctgcgtc actttcagtc atctcaatgg tgatattgtg gtcgcggaag aaacactgca
       61 tatagccggg atactggcca cgcgcctgaa catcaccaaa gaacatccag cgccggttct
      121 cttccatggc ctgcaacata tcctgtggct ggcaggtgag ggggtaaacc tgcccaccga
      181 gaagcatatt gccgattttc gcttcgggga tcaggctatg ataggcttta actgcccgcg
      241 cactggcaac cagttgatga tggatcgcct gataaacttc cgcctcgcca ctctcttctg
      301 ccagccccac gcccgtgaat ggcgcgtgta acgacatatt gatttcatta aacgtcagcc
      361 ataacgctac tttatgttgg tagcgggtaa agaccgtgcg ggcgtaatgc tcgaagtgat
      421 cgatgaccgc ccgattagcc caaccgccgt agtttttcac cagcccatat agcatttcgt
      481 aatgggataa cgttaccagc ggcttgatcc ccgcctgcgc catttcatca aacagccgat
      541 cgtaaaacgc taaccccgct tcattcggtt cggcttcgtc gccctgaggg aaaattcgcg
      601 cccaggcaat ggaaatacgc agacaggtga agcccatctc ggcaaataac gcgatatctt
      661 ccgggtaacg gtgataaaaa tcgatggcga catctttgat attctctttc cccaggatgc
      721 gcggttccat ttttcccatt acgccatgag gctgtaaatc tgaggtcgtg atccctttgc
      781 catcttccag ccaggcacct tccacctgat tggcagctgt tgcgccaccc caaagaaatg
      841 tttctggaaa tgctttcata attaactcct tttatcgtta gcgaatgatg gataacagcg
      901 gttcacctgc gcttatctgc gccgtgccgt ggggtaatac gtctgtaaaa tcatcgctat
      961 tactgattaa taccggcgtc gtcagatcaa atccggcctc gcgaatagca gggatatcaa
     1021 aagaaatcag ccgatcgcct gtattgacct tgtcacccac gttgacgtga gcggaaaaga
     1081 atttgccgtc cagttttacg gtgtcgatac cgacatgaat caggatctcc acaccatcat
     1141 ctgactcaat gccaatggcg tgtaatgtgg cgaacaacga agcaattcga cccgcaaccg
     1201 gagaacgcac ttcaccaacc gagggcagaa tggcgatacc tttacccaac aggccactgg
     1261 caaacgtggt atcagcgacg tgaatgagcg gcacaatctc tcccgtcatc ggtgaacaga
     1321 taccgccctg ctcaggtggt gtaataacct ctggtgtttt ctcttgcggg gcaccctgcg
     1381 ctggctgacg tttactggtg atgaaatgaa gcatcaacgt accaacaaat gcgcaaccta
     1441 tggcaatgac accgccaata acgctggccc agacggtgaa atcaatcccc gttgacggga
     1501 tggtctgcat gaaggtgaaa atacttggca aaccaaagga gtaaactttc gtttgcgcgt
     1561 agccaatgat ggtggccccc aatgctccac tgatacaggc gataacaaag gggtacttac
     1621 gcggcaggtt gacgccatat accgctggtt cggtgatacc aaacagaccc gtcaacgccg
     1681 ctgatcccgc caccactttt ttctgcgcat cgcgttcgca gaggaagacg ccgagcgccg
     1741 ccccgacctg cgccataatg gcgggcatta acagcgggat catggtgtcg tagcccagca
     1801 cggtgaagtt attgatacac agcggcacca ggccccagtg cagtccgaac atgacgaaga
     1861 tttgccagaa gccgcccatt accgcgcccg caaatgcagg aaccgcctga taaagccaga
     1921 gataaccggc ggcaatcatt tcgcttatcc aggttgatag cggccccacc agcagaaagg
     1981 tgacgggtgt gataaccatc agacatagca atggtgtgaa gaaatttttg attgccgacg
     2041 gtaaccacgc attaagtcgg cgttccagaa tgctgcacaa ccaggcagaa aaaataatgg
     2101 gaataaccga tgacgagtaa ttcaacaatg tgaccggaat acccaggaaa tccagccccg
     2161 gcgcatccgc tttttgcccg ttctcgaagg cagtcagaat taatggatgc actaacgctc
     2221 caccaatcac catggcagta aatggattac cgccgaagcg tttgcccgcg gtgtatccca
     2281 ggattatcgg gaagaaccaa aacaaggcat cactggcgct gaataaaatt aaataagtac
     2341 cactttgttc ggtcgtccac tgaaaagtga gcgccagagc cagcatacct ttcaggatcc
     2401 cggttgccgc catcagaccg atcagaggcg taaaaatacc tgaaataaca taaacaaagc
     2461 ggtttagcag attacctttc tcatcatttt ccggtgcctg ttgcgctttt tcgccaaggc
     2521 ctgccacgct gttaaccgcc aggaagacat cggccacatg gttacctatg accacctgaa
     2581 actgaccacc gctttccacc accataataa taccgggggt ctttttcagt acctccgctt
     2641 gcgctttgct ttcatccttt aacttaaaac gtaatcgcgt tgcgcaatgc atcagactca
     2701 caatgttatc tgcgcccccg actcctgcga ctatttttct ggctaactcc gtcataactt
     2761 gccctctacc gctttgcggc aaaactccaa aaaaaaacct gaaaaaaacg gcctgacgtg
     2821 aatcaagcaa tttttttcag gttttgcccg cttagtgcgg taacaatcct ttactcagta
     2881 ataatatttc agtgttcttt gcgcacgcgc tctatattta tggctaaaaa cataatctct
     2941 gcgggtgaaa ttttacgttg atactgcaaa ccaataaaaa tggcgatccg ttccgcacat
     3001 tgccatgctt gcgggtaatt ttgttttact gcttgttgta atgattcatc actatcgtta
     3061 attgaagcat gttcaagaat acgccaggat aaaaacttca gatgtgtaac cagtcgctga
     3121 taactcaagc tttcttcctg gtaattaagg ctgaactgaa attttattaa ttgcagcatt
     3181 tcgcgcatta actgcgtgac acctgcaaca tcctccatat ttccgctcat ttgggcactg
     3241 accagatgca tggcaataaa gcccacttca tctttcggta actgcacgcc caaccgttta
     3301 tcaataatgg ttaacgcttc
//
LOCUS       DADLSA010000261         3302 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.264, whole genome shotgun sequence.
ACCESSION   DADLSA010000261 DADLSA010000000
VERSION     DADLSA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.264"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(210..371)
                     /locus_tag="JN691_004141"
     CDS             complement(210..371)
                     /locus_tag="JN691_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245383.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(368..571)
                     /locus_tag="JN691_004142"
     CDS             complement(368..571)
                     /locus_tag="JN691_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY7245384.1"
                     /translation="MVERTMAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PYPLMQAIVAEGDVVVSGATEPE"
     gene            816..1151
                     /gene="iraM"
                     /locus_tag="JN691_004143"
     CDS             816..1151
                     /gene="iraM"
                     /locus_tag="JN691_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY7245385.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1432..1563)
                     /locus_tag="JN691_004144"
     CDS             complement(1432..1563)
                     /locus_tag="JN691_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
                     /protein_id="HAY7245386.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2063..2139)
                     /locus_tag="JN691_004145"
     tRNA            complement(2063..2139)
                     /locus_tag="JN691_004145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2103..2105),aa:Arg,seq:tct)
     gene            complement(2240..2315)
                     /locus_tag="JN691_004146"
     tRNA            complement(2240..2315)
                     /locus_tag="JN691_004146"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2280..2282),aa:Ile,seq:cat)
     gene            complement(2459..3280)
                     /locus_tag="JN691_004147"
     CDS             complement(2459..3280)
                     /locus_tag="JN691_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7245387.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT          951 a          879 c          606 g          866 t
ORIGIN      
        1 cgttcccacc gctgacgtgg tgtatgacac acctgttgtg attttttcca tctggtccat
       61 accccgtctc ccgttatccg gaagctgaca acaataaaaa agccaccagt taactactga
      121 tggctctgat aactcatgca agcgtctcag acgacccact gacactaccg gtgagtttaa
      181 cgataccttc catttgactg gctcactttt tatgatgatg ccggtgcatt tatctccagc
      241 accagacttt ctatctcaac gccatacgtt gcatttttgg taatatccgt cagcgtcagt
      301 gcatttagtc ccactgccag actgtctttt atggcctgga atgccgggcc agtacgatga
      361 cgtagtatca ctccggctca gttgcaccac tgaccaccac atcaccttct gctacaatcg
      421 cctgcatcag gggataaggg gttatggcca ccggactacc aaacggctgc cagccctctt
      481 tcagtttatg tgtcagcttt tccgcaagat ctgacggcgg cgccgccctg acaacatcat
      541 agtgtttaaa tgccatggtt ctttccacca tctgaaaaat aattctttaa aatacctgac
      601 atgtaataca gaaaaaacac aaaaccatac cttaaataaa aacctgatta tcaagcagat
      661 atgcatggat aaactacaag acgagatata aaccaccctg catttaaata aacaataaac
      721 aacatcagaa aaataattct gctctatggt ttaattcaaa aatatcattt attcttttca
      781 gaacatcacc agcaaggcat aaacaaggaa actaaatgaa gtggattgtg attgatacag
      841 ttatccagcc atcatgcgga atatcttttt cagtcatatg gagtaaaata aaattaataa
      901 tctggtatca atcggatgct ttcttacctc ctgaaagtat atttacactg actcacacag
      961 gcatcatgct caataacaaa gtgctacctg taaccattta caacgtagta ccattcaata
     1021 aaacattctg gaatttaatc aaaaacagcc aggaatgccc tacaaataca gataacgtat
     1081 tgaatgaatg ctttaataac cgttgcactc tgcaaatatg tccttatgga ctaaaacaac
     1141 aaagtccata aggagtttac tcacatctga caaaatcaat ataaacagcc cctccggaga
     1201 ggggctggag agtggcgcta tgtgccattg catggtgccg ggtgcctccc ggtgaattca
     1261 gtaccagcac ctgaatccgc gattatccca tatacctact cgctgattgc ccctccgcac
     1321 agggggattc accatgccag tttcttttaa caaactcccc gcaaaccaga caacagtcaa
     1381 ccgcctgaat tgtgaagtat ttaaaaattt ctcccgctaa ctgatacccg gctaacagtc
     1441 tggcgttttc tttttcagca acgggaaagc aacaaccacc acacccgcca ccagcacacc
     1501 gtcagccggc actgacatta tccggctgct gcaatgccat tcacaaaaac agtaagcaat
     1561 cactttttac cgtaacaggt gataatccag atatgtatct accccagatg agtaatccga
     1621 agttcatcca taccacaggt cctggctatt ctgttgtact cctgaacaag agcaaataat
     1681 tctgaattag caaccatgaa ctcatcgcaa accctctgta tagcatcact attcagaata
     1741 ataacgtctc ttcccgaaag acgatcagga gtacagaaca aaactgtcaa acggctgaag
     1801 gcctttgctc gtgctgcatt gactatatca atacgctgcc taaggatgaa acaccccgac
     1861 gcctcatcaa tattcactct acccacacca tatgaatgat aaatacttaa tgctgaaaaa
     1921 accattagac cgtataacaa atactcaatc aatacttaac agaactttta tttttgacaa
     1981 acatataata ttttcaacaa tatcctgagc caggtatatt tcagtataag gctctgccgg
     2041 aaggaatctg gaagaatgaa tatggcgcgc tgtactggat tcgaaccagt gaccgattgc
     2101 ttagaaggca attgccctgt ccggctgagc taacaacgct gaataccgat aatggaccgc
     2161 catcggggac ccgcccccgc accaacaacc ctgttatcgt gtcgtctgct cttcctgata
     2221 agctaatggc ggtttgtgat ggtggccctt gctggatttg aaccagcgac ctggcgatta
     2281 tgagtcgctc gctctcacca ctgagctaaa gggccgggag cagaataata acggtccgta
     2341 attaatttcg caataaaaaa cccgctcagc ggcgggttgt agaaactctt ctaacgtcag
     2401 gcataaaacg cccatcatta tgacgaattt accacagatt ccggaaaaat caaccttgtt
     2461 acctagttac cttttttaac tgccgctcag cccatgcttc ttcaatatca aaccgggtca
     2521 ccagcgcatc ataaaatttc ttaactgttt tttcccatga cgcgcgtgtt atctggtttg
     2581 tcacctcgca tatagcatta aatgcctccg ttgatggtag tctttcatag ccacgaccac
     2641 cacaacgctg gcagtctctg ataacaggca taccacgttt taccgactct tcacgatgaa
     2701 tggcgacacc acgcccacgg caatccttac aggcggtgga aacctcaccc tttccgccac
     2761 actccggaca ggcaactttt accacctccc tgactttttt ccattcttcc cagtaagacg
     2821 gatacacacc tttcgtacac tttgcccata ccggcggctt accatccgga tactggatct
     2881 tatttgtaaa aactatgctt tcaataaatt tttccccata gcaacaaggg cactgctttt
     2941 tactcgctgc gctgcgggca taatcctcaa aagcgtacga agccataatg cgcatcacta
     3001 ccggttttat ttctgccgga agttttctca acgccgccac acgatcgcac cgactgagtg
     3061 cataatctgc cagtaattct gttgcccgcg ccctgtcatt catactgatg cccattttcc
     3121 ccaggaacgc agaaaaaccc atctcagccc gattctgtgt catgccctgc gcggccatca
     3181 catcagtgat actcagcgca tcttttgacg ttgaggccga tgcatcggtc aggccagggg
     3241 attttgggga gtagtatttc ggtaaatctt ccagtttcat tttttgacct gctcttaatg
     3301 ca
//
LOCUS       DADLSA010000262         3281 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.265, whole genome shotgun sequence.
ACCESSION   DADLSA010000262 DADLSA010000000
VERSION     DADLSA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.265"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            143..391
                     /locus_tag="JN691_004148"
     CDS             143..391
                     /locus_tag="JN691_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245388.1"
                     /translation="MNNPIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            724..2247
                     /locus_tag="JN691_004149"
     CDS             724..2247
                     /locus_tag="JN691_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7245389.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            2379..2598
                     /locus_tag="JN691_004150"
                     /pseudo
     CDS             2379..2598
                     /locus_tag="JN691_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2998..3207
                     /locus_tag="JN691_004151"
     CDS             2998..3207
                     /locus_tag="JN691_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245390.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
BASE COUNT          910 a          642 c          700 g         1029 t
ORIGIN      
        1 gtgttgcgat acacagctga tgatatgtaa cttctaatta ttaagtataa gtttatagaa
       61 aactcattca tcttattttt gtctgtcgct ttagactata cacagcataa ttttattggg
      121 ttaatatttc tacgaggctg acatgaataa tccaatccca gagagattta tttttcaatg
      181 tgctttgttt aaaaatctcg agagggaggt gttcatgacg catgggtatg ttgatagtca
      241 tattattgat caggctttac gcttgcgttt aaaagatgaa accagtgtga ttctttctga
      301 tctctatctg caaatattgc agtacattga aatgcataag actacactaa cggatatcat
      361 tattaatgac agggaatccg tgctctctta gaacttgagt aaggttttag aataaacatc
      421 agctgtatca ccatgctgat gcaaagtgag catttcaggc gttatgcttt cttattatgt
      481 ccgcaatatc aggtgtcaag aatggagagt tctcgctctc cattcttgac acctgatatc
      541 ccgcctaact tattttgtac tgcctacatt atgctcaata attaatgggt tagatcaaga
      601 tggagaaaat tttatcttcg gcgtggatat ttcttatgct ctctaccatg catctctgta
      661 gatgccagcg cgagcgaatg aatggcattt tcatgtatta aaccctttgg ccgggacaag
      721 caaatgcgca atacactcat acccatcctc gttgcgatat gcttgtttat taccggtgtc
      781 gctattttga acatccagct ctggtattcc gcaaaagcag agtacctggc gggagcaaga
      841 tatgccgcca acaatatcaa tcatatactt gaagaagcgt cacaggcgac tcaaacagca
      901 gttaacattg ccgggaagga atgcgacctc gaggagcaat atcagcttgg cactgaagca
      961 gctctgaaac ctcacctgcg cacaatcatc attctcaaac agggaatagt ctggtgtaca
     1021 tccctgcctg ggaatcgggt cctgctgtct cgtattcctg ttgtcccgga cagtaattta
     1081 ctgttggctc cggcaatcga caccgttaat agattaccta tcctgctcta tcagagccaa
     1141 tttgcagata cgcgcatttt ggttacgata agtgatcaac atattcgcgg ggcgcttaat
     1201 gtacccttga aaggggtaag gtatgtatta cgcgtggcgg atgacattat tggaccaacg
     1261 ggtgatgtga tgacgcttaa tggacattat ccctataccg agaaggttca ctccacaaaa
     1321 tatcatttca ctattatctt taacccgccg ccactcttta gcttctacag acttatcgat
     1381 aaaggctttg ggattttaat atttattctg ttaatcgcct gcgccgctgc cttcctgctt
     1441 gatagatatt tcaataaaag cgcaacgcct gaagagatcc tgcgacgggc aataaataat
     1501 ggggagatcg tcccttttta ccaacctgtg gtaaatggtc gggaagggac attgcgggga
     1561 gttgaggtgt tagcccgctg gaaacaacct cacggtggat atatttcacc cgcggcattt
     1621 attccacttg ctgaaaaatc cggattaatc gttccgctta cacaaagcct gatgaatcag
     1681 gttgccagac agatgaacgc tatcgcgagt aaactgccgg aaggttttca tattggaatt
     1741 aattttagcg cttcgcatat tatttcgccg acgtttgtcg acgagtgttt aaatttccgt
     1801 gacagtttta cccgccgcga tttaaacctt gttctggaag tcaccgagcg tgagccattg
     1861 aatgttgatg aaagtctggt tcagcggttg aacattctgc atgaaaatgg ttttgtcatc
     1921 gcgctggatg atttcggtac tggctactca gggctttctt atcttcatga cctgcatatt
     1981 gattatatca aaattgatca cagtttcgtt ggccgcgtaa acgcagaccc agaatcaacc
     2041 cgaattctgg attgtgtatt ggatctggcg cgtaaacttt cgatcagtat cgtcgctgaa
     2101 ggtgtcgaaa cgaaagaaca acttggctat ctgaaccaaa ataatatcac atttcagcag
     2161 ggttattatt tctataaacc tgttacatac atcgacctgg tcaaaattat cctttctaaa
     2221 ccgaaggtga aggttgtggt tgagtgaaaa ttgatcagta aggccatagt gcggtgtaat
     2281 tatagacagc taattagctc gttgcctctt gttactattg ttcattattt tgtttgctat
     2341 aattgtttga aagttttgac aggattgcca ttagtagcat gaacaatagt aataatctgg
     2401 attgtttcac tctctatatc atcttttcca ttgcatttat gctgatcacc ctcctggtca
     2461 tccttattgc aaaacccagt accgggctgg gagaagtgct tgtgacgata aatttgctta
     2521 atgcccttgt ttggctggcg atcaatctgg ttaatcgatt taagagaaag actcgtcagc
     2581 cacagggatc agcaataatc tttcagtttc tcactgtcag tatgcggctg aatgggttgc
     2641 tggcagtgaa cgcctggatc attgaaggaa aggaattatt gcgcaaatag ttgtcaacct
     2701 tggtgttatc acagttgttt ttatatatca ccgaaataat taccatggca actattaata
     2761 attttgatgt cgaagagtta tttgttaaac aaaatagtca ccccaaagtg atcaatgtca
     2821 tgaaaataag gtgaaaaatg ataatgccga cttatttatc atttatatat tgtcgctgtt
     2881 tatctattta aattttttgt gcttttgttt ttgtctttac cctccgtttt ggtaatctga
     2941 gctcataggt tatttgacca ggaatgtttg ttaatccaat gaatggatgg ttaatctatg
     3001 aaactgaaaa aactccccgg gtttagtttg ggacttattg ctctggcggt gggtaatgca
     3061 tatgctgaac agtttattca gcacgatgaa tataaacatg ctgtggcaat aaaagatgga
     3121 aaagtaaaag atattgataa tgcgctagtt ataacaacgg gtaatggaag ttacgggtat
     3181 ccgttgcagg agagggtagc aagttaacca ttaacaatgg cgtcattgta acaacgggcg
     3241 gtttatatcc ttctgacaat agtcctctaa gcccagcatt a
//
LOCUS       DADLSA010000263         3264 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.266, whole genome shotgun sequence.
ACCESSION   DADLSA010000263 DADLSA010000000
VERSION     DADLSA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.266"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..263)
                     /locus_tag="JN691_004152"
     CDS             complement(<1..263)
                     /locus_tag="JN691_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245391.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSV"
     gene            288..527
                     /gene="relB"
                     /locus_tag="JN691_004153"
     CDS             288..527
                     /gene="relB"
                     /locus_tag="JN691_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HAY7245392.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            527..814
                     /gene="relE"
                     /locus_tag="JN691_004154"
     CDS             527..814
                     /gene="relE"
                     /locus_tag="JN691_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HAY7245393.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            886..1041
                     /gene="hokD"
                     /locus_tag="JN691_004155"
     CDS             886..1041
                     /gene="hokD"
                     /locus_tag="JN691_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HAY7245394.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1259..1510
                     /locus_tag="JN691_004156"
     CDS             1259..1510
                     /locus_tag="JN691_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245395.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVASLQALKEIGIKAGFSAFSEE"
     gene            1577..1855
                     /locus_tag="JN691_004157"
     CDS             1577..1855
                     /locus_tag="JN691_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245396.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
     gene            1857..2906
                     /locus_tag="JN691_004158"
     CDS             1857..2906
                     /locus_tag="JN691_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY7245397.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRLLVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLLFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            2919..>3264
                     /locus_tag="JN691_004159"
     CDS             2919..>3264
                     /locus_tag="JN691_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7245398.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYP"
BASE COUNT          831 a          772 c          871 g          790 t
ORIGIN      
        1 aacactgaca tgctaaagca aaaaatagat gaataagttg agttgtgcat atgtagcctg
       61 accgtcacaa agtatatggt gtctgtacca gtaagatgat ggccggactc tttaaaaaag
      121 agctgacctg cacaatacag gatggactta gcaatggctg ctcctggcac aaagcggaca
      181 gtgatcaccg ttcttacgac tactttctga cttccttcgt gacttgccct aagcatgttg
      241 tagtgcgata cttgtaatga catttgtaat tacaagaggt gtaagacatg ggtagcatta
      301 acctgcgtat tgacgatgaa cttaaagcgc gttcttacgc cgcgcttgaa aaaatgggtg
      361 taactccttc tgaagcgctt cgtctcatgc tcgagtatat cgctgacaat gaacgcttgc
      421 cgttcaaaca gacactcctg agtgatgaag atgctgaact tgtggagata gtgaaagaac
      481 ggcttcgtaa tcctaagcca gtacgtgtga cgctggatga actctgatgg cgtattttct
      541 ggattttgac gagcgggcac taaaggaatg gcgaaagctg ggctcgacgg tacgtgaaca
      601 gttgaaaaag aagctggttg aagtacttga gtcaccccgg attgaagcaa acaagctccg
      661 tggtatgcct gattgttaca agattaagct ccggtcttca ggctatcgcc ttgtatacca
      721 ggttatagac gagaaagttg tcgttttcgt gatttctgtt gggaaaagag aacgctcgga
      781 agtatatagc gaggcggtca aacgcattct ctgaaccaaa gcatgacatc tctgtttcgc
      841 accgaaggtg acacttctgc tttgcgttga caggagaagc aggctatgaa gcagcaaaag
      901 gcgatgttaa tcgccctgat cgtcatctgt ttaaccgtca tagtgacggc actggtaacg
      961 aggaaagacc tctgcgaggt acgaatccga accggccaga cggaggtcgc tgtcttcaca
     1021 gcttacgaac ctgaggagta agagacccgg cgagtgagaa atccctcgcc acctctgatg
     1081 tggcaggcat cctcaacgca cccgcactta acccgcttcg gcgggttttt gtttttattt
     1141 tcaacgcatt tgaagttctg gatggtgccg gaatagaatc aaaaatactt aagtagcgcg
     1201 cagggataaa agggatggac cccgaacagg ggagtgctac ttatctggaa ggattctgat
     1261 gatgaacatc gaagaactgc gtaaaatttt ttgtgaagat ggcctctatg ctgtgtgcgt
     1321 tgaaaatgga aatattgtta gtcattaccg cattgtgtgt ttgcaaaaaa atggggctgc
     1381 gttaattaat tttgtggatg cccgagtgac ggacggattt atcttgcgcg acggtgagtt
     1441 tgtcgcttca ttacaggcat tgaaagagat cggaataaaa gctggctttt ctgctttttc
     1501 agaagaataa actcatctac aatcttgcgc ggggctgaac tcccgctgag taacaccgtg
     1561 ccaccggaga aaaccgatgg cacgcaacgc aaaatattac aatcatgata attcgaccgt
     1621 tcttgcccac acgcacgagc ggtattctca cgcatttaag tcagactggt accagcatcc
     1681 ctcatgcact gaagaacagg ccgaatggct cattcagtgt taccgcaggc gcggatgcga
     1741 ggttaaaaaa gcccttagcc tcgactaccg tcactggata atctccgtca ggctccctta
     1801 ctccgaacag ccaccgcgtc cgtcccgcac attccagcaa cggatctgga ggtaacgtgc
     1861 gggtattact tcgacctgtt ctggttccgg aactcgggct ggtggtcctt aagcccggtc
     1921 gtgaatccat gccggtattc cacaataccc ggttactggt ggagccggaa ccgaaaagca
     1981 tgcgtaatct gccgtccggg gtcgttcctg ccgctcgcca gccgctggtg gaagacaaaa
     2041 cattgctgct gtttttcagt aacgcacggg tgattcgtgc tgctggtggt gctggtgcat
     2101 tgtctgactg gctgttgcgc catattaaat cctgccagtg gccacacggc gattatcatc
     2161 acagcgaaac cgtcattcac cgttatggta ccggcgcaat ggtgttgtgc tggcactgcg
     2221 acaaccagct gcgtgaccag acatccgaat cactcgagca acttgctcat caaaacctgt
     2281 cagcatggat gattgacgtc atcggtcacg caataagcgg tacgcaggag cgtgaattat
     2341 ctctagctga attatcctgg tgggcggtcc gcaatcaggt ggcggacgcg ctaccggaag
     2401 cggtattacg tcgttcgctg gggttgcgtg cggaaaaaat ccgctcaatg taccgtgaaa
     2461 gcgacatcgt accgggagag cagaccgcca ccagcatact gaaacagcgc acaaaaaatc
     2521 ttgcgccgct gcctcacgcc caccagcaac agaacccacc acaggaaaag acggtggtca
     2581 gcattgccgt tgatcctgag tctccggaat ctttcatgaa acgacctaaa cgtcgccgct
     2641 gggttaacga gaaatacaaa cgctgggtga agacacagcc gtgtgcgtgt tgtggtaagc
     2701 cagccgacga tccccatcac ctgattggtc atggtcaggg cggaatgggg acaaaatctc
     2761 acgatatttt cacgctaccg ctgtgtcggg agcatcacaa cgagcttcat gcggatcctc
     2821 tggcgttcga agaaaagcat ggttctcagg ttgatttaat ttttcgtttt cttgatcacg
     2881 cctttgcaac tggcgtgctt gggtaaaaga ggtgactgat gctcatagat ttggttttac
     2941 cttacccgcc gacggtgaac acttactggc gacgccgtgg cagcacatat tttatctcgg
     3001 aggagggaaa gcgttatcgc cgggctgtgg cgcttattgt tcgccagcag cggctgaaat
     3061 taagcctgtc cggaaggctg gcgataaagg tgattgcaga gccaccggat aagcgtcgtc
     3121 gcgacctgga caatatcctg aaagcaccgc tggatgcgct gacgcatgcg ggagtgttaa
     3181 tggacgatga gcagtttgat gaaatcaata tcgttcgtgg tcagccagta tctggtggac
     3241 gtctgggggt gaagatttac ccca
//
LOCUS       DADLSA010000264         3218 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.267, whole genome shotgun sequence.
ACCESSION   DADLSA010000264 DADLSA010000000
VERSION     DADLSA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.267"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            80..343
                     /locus_tag="JN691_004160"
                     /pseudo
     CDS             80..343
                     /locus_tag="JN691_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            970..1515
                     /gene="cobU"
                     /locus_tag="JN691_004161"
     CDS             970..1515
                     /gene="cobU"
                     /locus_tag="JN691_004161"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAY7245399.1"
                     /translation="MMILVTGGARSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDDGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            1512..2255
                     /gene="cobS"
                     /locus_tag="JN691_004162"
     CDS             1512..2255
                     /gene="cobS"
                     /locus_tag="JN691_004162"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAY7245400.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            2267..>3218
                     /gene="cobT"
                     /locus_tag="JN691_004163"
     CDS             2267..>3218
                     /gene="cobT"
                     /locus_tag="JN691_004163"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY7245401.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQVGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLG"
BASE COUNT          758 a          683 c          903 g          874 t
ORIGIN      
        1 acaactagtg atagttgact tattttcgct cttgagagac aaccatactc aaatctccca
       61 cattgcagga gatttgaata tgaaaacgtc accgtggaat aaagaccgta ccatcggtca
      121 aaaaaggcca cttcagatat ctcatatctg ggggatccgg attagacttg agttggaagg
      181 taaaacacgc gatctagctc tgttcaacat ggctttggac agtaagctac ggggctgtga
      241 tttggtcaaa ctcaaagtat cagatgctgc atatggcagt tctgtctcaa gcagagcaac
      301 ggtgttgcaa cagaaaaccg gcagccctgt ccaatttgag atgcctttaa taacacatct
      361 tttaaatgtt gaagaattat cccgcctgct gaagaacgtt gctctgagtg tgataaacct
      421 ttttattctt gatgatgtca gcgagaagat ggaatatatc cccgactggg tcgtggttac
      481 ccggttaaac atctggtggc gctaataaat ctggcaagtc acattctgtt gaatgctgtg
      541 acagtgcctt accgagagag cgaaattgtg gtgttctagt gcatagtcgc caccattccg
      601 gataattcta ttgcgttgtt ttacaagttc ttttacagcg cagatctgtc gctgacgttg
      661 aatcaatagc ctggatttca gaacacaatg cccgtttgct ggttggtccg gcagtagcat
      721 taattcatta tctgaatgaa atgtgtcaat ccacagaaac cgctgtcaat gacgcatttt
      781 gtcggacgtt aacacgagaa agtctggcta ttccgtaatg ccatcttatt ttgtaatgga
      841 ttgagaatgc tggattttta attatattat gcgcagtatt aatcgcgtaa atgttttgcg
      901 gtcagcaacg gtgggggatc cattgctctg ttcaatgaat accatcatac atttgaatca
      961 ggagtcggta tgatgatttt ggtgacggga ggcgcacgga gcgggaagag tcgccacgca
     1021 gaggtgctta ttggggactc ttcacaggtt ctgtatatcg ctacctcgca aatccttgat
     1081 gatgagatgg ctgcacggat agaacatcat cggcaaagcc gcccggagca ctggcgcaca
     1141 gtggagcgct ggcaacatct tgatgaatta attcatgcag acattaaccc gaatgaggct
     1201 gtgttgctgg aatgcgttac cacaatggtg actaatctgt tgtttgatga tggcggcgat
     1261 aaagaccctg atgaatggga ttatcaggcg atggaacagg cgattaatgc tgagattcag
     1321 tcgttgattg ctgcctgcca acgttgcccc gcaaaggttg tattagtgac taacgaagtg
     1381 ggaatgggga ttgtgccgga gagtcgtctg gcacgacatt ttcgtgatat tgccgggcgg
     1441 gtaaatcagc agttggccgc tgcggcaaat gaagtatggc tggtggtttc gggtattgga
     1501 gtaaaaatca aatgagcaaa ttattttggg cgatgctctc atttattacg cgtctgcctg
     1561 taccgcgtcg ctggtcccag ggactggatt tcgagcatta ttctcgcggt attattactt
     1621 ttcctttgat tggattattg cttggcgcga ttagcgggct ggtcttcatg gtgctgcagg
     1681 catggtgtgg cgcaccactg gcggcactgt ttagcgtact tgtgctggcg ctgatgaccg
     1741 gggaattcca tctggacggc ctcgctgata cctgtgatgg cgtattttct gcacgtagcc
     1801 gcgatcgcat gttggaaatc atgcgtgata gccgtttagg cacccacggc ggtctggcat
     1861 tgatttttgt ggtactggca aagattctgg tgttaagcga gttggctctg cgtggcgaac
     1921 cgatccttgc atcgctggcg gcagcatgtg cggtcagccg cgggactgcc gcattattga
     1981 tgtatcgtca tcgttatgcg cgggaagaag ggcttggcaa tgtgttcatt ggcaaaattg
     2041 atgggcgaca aacctgcgtc acactcggct tagccgctat ttttgctgcc gtattattgc
     2101 cgggtatgca tggtgtggct gctatggtgg tgacgatggt ggcgattttc atcctcggtc
     2161 agcttctcaa acgtacgctg ggcgggcaaa cgggcgatac gctgggagca gccatagagc
     2221 ttggtgaact ggtcttcctg ctggcactgc tataaagaga cgtgacatgc aaataattgc
     2281 cgatttactg aatacgatcc cagccatcaa ttctgccgct atgtcgcgtg cacaacggca
     2341 tgttgacggg ttactcaaac ctgttggtag cctaggaaag ctggaggcgc ttgccataca
     2401 actggcagga atgccggggg tgaatggcat accgcatgtg ggcaaaaaag cggtactggt
     2461 tatgtgtgcc gatcacggcg tctgggagga aggggtcgct atttctccaa aagaagtgac
     2521 agccatacag gctgaaaata tgacccgtgg aacaaccggc gtgtgtgtgc tggcagcaca
     2581 agtgggcgct aacgtccacg taattgatgt tggtattgat actgctgagc ctatccccgg
     2641 gcttatcaac atgcgtgtcg cacgaggtag cggcaatatt gcttcagctc cggcaatgag
     2701 tcgccgtcag gctgaaaagt tgcttttgga cgtcatatgt tatacgcggg agctggcaaa
     2761 aaacggtgtc acgctgtttg gtgtaggtga actggggatg gcaaacacga caccggcagc
     2821 ggcaatagtc agcacaatca ctggccggga tcctgaagaa gtggttggga ttggtgcaaa
     2881 cctgccgaca gataaactgg ctaataaaat tgatgttgtg cgtcgggcga ttacgttgaa
     2941 tcaaccaaat cctcaggatg gtgttgatgt cctggcaaaa gtgggtggat ttgatttggt
     3001 cggaatagct ggagtgatgt taggtgctgc ttcctgcggt ttacccgtgt tgctggatgg
     3061 atttctttct tatgctgctg cgctcgcagc ctgccagatg tctcctgcaa tcaaaccgta
     3121 tctcattcct tctcacttgt cggcagaaaa aggcgcgcgt atagcgctct cgcatttggg
     3181 gctggagcct tatctcaata tggatatgcg tttaggtg
//
LOCUS       DADLSA010000265         3218 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.268, whole genome shotgun sequence.
ACCESSION   DADLSA010000265 DADLSA010000000
VERSION     DADLSA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.268"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..1166
                     /locus_tag="JN691_004164"
     CDS             <1..1166
                     /locus_tag="JN691_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAY7245402.1"
                     /translation="IGGDKSIPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILR
                     AASFGNAQLMIPMVHSLDQILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCY
                     IIDHFCDEVDFFSIGSNDMTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQR
                     GKWVGICGELGGESRYLPLLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQAC
                     ECRSAQEIEALLTAFTPEEDVRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEH
                     PFELEEDVWQREEIVTTGVGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVEL
                     VIMLTLGANEGMNHVKVFSQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(1169..2026)
                     /locus_tag="JN691_004165"
     CDS             complement(1169..2026)
                     /locus_tag="JN691_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7245403.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2039..2773)
                     /locus_tag="JN691_004166"
     CDS             complement(2039..2773)
                     /locus_tag="JN691_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7245404.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(2788..>3218)
                     /locus_tag="JN691_004167"
     CDS             complement(2788..>3218)
                     /locus_tag="JN691_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY7245405.1"
                     /translation="KDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAIVH
                     GIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNVHH
                     RVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT          781 a          837 c          839 g          761 t
ORIGIN      
        1 acatcggcgg cgataaaagc attccttatc tgaatattcc ccaggaagag aacccgttcc
       61 tcggctatcg cgcggtacgt atttacccgg aatttgctgg cctgttccgc actcaactgc
      121 gggccatttt gcgcgccgcc agtttcggca acgcccagtt gatgatcccg atggttcaca
      181 gcctcgatca gatcttatgg gtgaaaggcg agatccaaaa agcgatcgtt gagcttaagc
      241 gcgatggcct gcgtcatgca gagacgatta cgcttgggat catggtggaa gtgccgtcgg
      301 tgtgctacat catcgaccac ttctgcgatg aggtcgattt cttcagtatc ggctccaacg
      361 atatgaccca gtatctgtat gcggtcgatc gtaataaccc gcgcgtatcg ccgctatata
      421 acccgattac gccatcgttc ctgcgcatgt tgcagcaaat agttaccact gcgcatcagc
      481 ggggcaaatg ggtaggcatt tgcggtgaac tgggcggtga aagccgttat ctgccgctac
      541 tgcttgggct gggcctggat gagctgagta tgagtagccc gcgtattccg gcggtgaaaa
      601 gccagcttcg tcaactggat agcgaggcgt gtcgggaact ggcgcgtcag gcatgtgaat
      661 gccgcagtgc gcaggaaatt gaagcgctac tcaccgcctt tacgccggaa gaagacgttc
      721 gcccactgct ggcgctggag aatatctttg ttgatcagga ttttagcaat aaagagcagg
      781 cgatccagtt cctgtgcggc aacctcggcg ttaacgggcg cactgaacat ccgtttgagc
      841 tggaagaaga tgtctggcag cgggaagaga ttgttaccac cggcgttggt tttggcgtag
      901 cgatcccgca caccaaatct cagtggatcc gtcattccag tatcagcatt gcccggctgg
      961 cgaaaccggt tgactggcag tcagaaatgg gcgaagtcga actggtgatc atgctgacgc
     1021 tgggtgctaa cgaagggatg aatcatgtga aagtcttctc gcagctggcg cgtaaactgg
     1081 tgaataaaaa cttccgccag tcgttgtttg ccgcgcaaga tgcacaaagt atcctgacgc
     1141 tgctggaaac agaattaacc ttctgacgtt agccctgaaa acgggcgctg tactctcccg
     1201 gcgtcagacc aaactgacgc cggaaaacgc gacaaaaata gtcgctatcc ggaaaaccgc
     1261 aacgctgtgc cacttcatga attgacagat gatatttctg caaaatcatc ctcgccttcg
     1321 ccattcgcac ccaacgcaca tactcgataa aacgcatcgt tccatgctga gcaaacagtt
     1381 ttgacagatg attgggcgtg atattaaaaa actgcgccac gctctcgcgg gtgagcggct
     1441 gggcataatt atcctgaacc cagttacaaa tgctgtgata gagaaaatcg ccgcgcggtt
     1501 gcgaattacc cggcggcata ttcaccacgt tgcggcaaag atgcagcaaa cttaacacca
     1561 gtggctgaat gatattctgc tccagcggcg aacggctaag atgggtgagg gcggtaagca
     1621 tcgcttcacc ttcaccgcgt tgcaggctgg gaagctggat ctggcgagca ggatgcaaca
     1681 acgacgtggt gcggctatcg taaaacgata acccaagcca ggtcggtgcg aacaccaggc
     1741 ttaacagcat caccggtttg ttgttgatcg gtaaattagc cgcgcgtgcc ggaataaaca
     1801 gcatttctcc ttccaccatt cgctgctgct ttgcttccag caggttgccg tattcgccgc
     1861 gcagcacgat atccagacgg ggaaaatcaa tacataaact gcccaccggt aacgaggcag
     1921 gctggctggc aaaccagacg cgccccagta gttgcgggtt aagcaccagg ccgctgaaca
     1981 gatcggcgaa aaattgttga tcggcgggca agcctggtgc tttcatgaaa gttcccgatt
     2041 aaagatgcat taactggcga aattctttca ctttgctgcg gctgaccggc acttcaaaat
     2101 ccagatcttt cagtcgcaga atgtaggtgt tattaaacca cggttcgatt tcgcgtattt
     2161 tgttcagatt gacacaaaat gagcgatggc agcggaaaaa atgcgacggc ggcagtttgc
     2221 tgcaaaattc ggtaatgttc atcggcatta cgtaggattc acgccgcgta tagacaaagg
     2281 tcattttctc gtgcgcttcg gcgtaataga tatcgttaat tggcgtgacg attattcgct
     2341 catctttcac cagattaatg gtgtcattct cacgcgttac cgtcgcggca ggcgtactgc
     2401 tggtctgctg ttgttgccag gccgcttcca gtttttgcag catcccggta atacgtgact
     2461 cctgatacgg tttgagaatg tagtcaaacg cctccagttc aaacgcttct accgcatgtt
     2521 ctttccacgc ggtgatgaac acaataaacg gtttatgggc gaactggctg atgttttgcg
     2581 ccagcaacac gccatccagc gacggaatat tgatatccag aaaaatggcg tcgacgcggt
     2641 tatgctgcaa aaacttcaac acgtccagac cgtcgtcaaa ggtgccgaca atctccatct
     2701 ggctgtgctc tttaattagc cagctcagtt cctgttgtgc caggaattcg tcttcaacaa
     2761 tgatgacttt cacaatatca ctccggctca aagcaataac gtagcctgtg aggcgactgg
     2821 ggtgcgttgg ttaggaatgt aaaacgcaat ttccgtcccc ggctccaggc ggcggatatg
     2881 cagcccctcg ccatacaata acttcacgcg atgatggaca ttcagcaggc caattttatt
     2941 gcccggcatt tcattcgctt cgacccgctc aatcaccttt ggatcgatgc cgtgcccggt
     3001 atctcgcacc gcaatgcgta cccgatttcc gcactctgca acgctgatgg tgacaacgcc
     3061 tttacctttg caaggctgaa taccgtggac aatggcgttc tccaccaacg gctggatcag
     3121 caggctggga atgcagcaat tcacctcttc atcaatatca tagataaccg tcagcttgtc
     3181 gccaaagcgg gcctgctcaa tggcaatata gtctttaa
//
LOCUS       DADLSA010000266         3197 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.269, whole genome shotgun sequence.
ACCESSION   DADLSA010000266 DADLSA010000000
VERSION     DADLSA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.269"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            20..610
                     /locus_tag="JN691_004168"
     CDS             20..610
                     /locus_tag="JN691_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY7245406.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            850..1110
                     /locus_tag="JN691_004169"
     CDS             850..1110
                     /locus_tag="JN691_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAY7245407.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1334..1408
                     /locus_tag="JN691_004170"
     CDS             1334..1408
                     /locus_tag="JN691_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAY7245408.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1401..2258
                     /gene="repA"
                     /locus_tag="JN691_004171"
     CDS             1401..2258
                     /gene="repA"
                     /locus_tag="JN691_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY7245409.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2958..3098
                     /locus_tag="JN691_004172"
     CDS             2958..3098
                     /locus_tag="JN691_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245410.1"
                     /translation="MAENGYGLAGLGVGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CR"
BASE COUNT          824 a          802 c          823 g          748 t
ORIGIN      
        1 tgccaggagc agataaataa tgaagttgat tatttttatt ctcattgttc tgattattgc
       61 agcgctactt atccggatta tcctcagaag tgtaaatcag cattctcctt tgctgatgca
      121 attgcatgcg gcaggtatac ggaccggtga tgcagagcga atactgtcca gcggtgaata
      181 ctggcaacgt cagaagacgc tgctgacaga aagggaagtc agttttatga aaggactgtt
      241 cagaattgtg gatatgaagc ggtggtatct gtgtccgcag gtacgggtcg cggatatcgt
      301 ccagctgaac gggaatatcc ggccacgatc acgccagtgg tggcagttat tcaggatggt
      361 gtctcagtgg catgttgatg tggttatcgt tgagctgcgt tcgttcagta ttgttgcagc
      421 agtagagctg gatgatgcca gccatttacg accggaacgc agacgccggg atattcttct
      481 ggaagaggtt ctgaggcagg ctggtattcc gttgctcaga agccacgatg ccagaaaact
      541 gctgcagatg accggagaat ggctgaatac aacaggggct gatcagcagt caccggaaca
      601 tcgtagctga cgccttcgcg ttgctcagtt gtccaacccc ggaaacggga aaaagcaagt
      661 tttcctcgct cccggcgttt ccataactga aaaccatact atttcacagt ttaaatcaca
      721 ttaaacgaca gtaatccccg ttgatttgtg cgccaacaca gatcttcgcc acaattctca
      781 agtcgctgat ttcaaaaaac tgtagtatcc tctgcgaaac gatccctgtt tgagtattga
      841 ggaggcgaga tgtcgcagac agaaaatgca gtgacttcct cattgagtca aaagcggttt
      901 gtgcgcagag gtaagcctat gactgactct gagaaacaaa tggccgctgt tgcaagaaaa
      961 cgtcttacac acaaagagat aaaagttttt gtcaaaaatc ctctgaaaga tctcatggtt
     1021 gagtactgcg agagagaggg gataacacag gctcagttcg ttgagaaaat catcaaagat
     1081 gaactgcaga gactggatat actaaagtaa agactttact ttgtggcgta gcatgctaga
     1141 ttactgatcg tttaaggaat tttatggctg gccacgccat aaggtggcag ggaactggtt
     1201 ctgatgtgga tgtacaggag ccagaaaagc aaaaaccccg ataatcttct ttaactttgg
     1261 cgagtcagaa agattaccgg ggcccactta aaccgtatag ccaacaatca agctatgcgg
     1321 ggagtatagt tatatgcccg gaaaagttca agacttcttt ctgtgctcgc tccttctgcg
     1381 cattgtaagt gcaggatggt gtgactgatc ttcaacaaac ttattaccgc caggtaaaga
     1441 acccgaatcc ggtgttcaca ccccgtgaag gtgcccggac gcttccgttc tgcgggaaac
     1501 tgatggaaaa ggcggtgggc ttcacctccc gttttgattt cgccattcat gtggcacatg
     1561 cccgttcgct tggtctgcgt cggcgcatgc caccggtact acgtcgccgg gccattgatg
     1621 cgctgctgca ggggctgtgt tttcactatg acccactggc caaccgcgtc cagtgttcca
     1681 tcaccacgct ggccattgag tgcggactgg cgacggagtc tgctgccgga aaactctcca
     1741 tcacccgggc cacccgggcc ctgacgttcc ttgcagagct gggactgatt acctaccaga
     1801 cggaatatga cccgcttatc gggtgctaca ttccgaccga tatcacgttc acatctgcac
     1861 tgtttgctgc cctcgatgta tcagaggagg cagtggccgc cgcgcgccgc agtcgtgtgg
     1921 aatgggaaaa cagacagcgc aaaaagcagg ggctggatac cctgggtatg gatgaactga
     1981 tggcgaaagc ctggcgtttt gttcgtgagc gtttccgcag ttaccagaca gagcttaagt
     2041 cccgtggaat gaagcgtgcc cgtgcgcgtc gtgatgcgga caggcagcgt caggatattg
     2101 tcaccctggt gaaacggcaa ctgacgcgtg aaatctcgga agggcgcttc actgccagtc
     2161 gtgaggcggt aaaacgtgaa gtggagcgtc gtgtgaagga gcgcatgatt ctgtcacgta
     2221 accgtaatta cagccggctg gccacagctt ccccctgaaa gtgacctcct cagaataatc
     2281 cggcacgcgc cggaggcatt cgcacgcctg aagcccgcca gtgcacaaaa aatcagcgct
     2341 acatacaaaa aacaacctca tcatccacct tctggtgcat ccggtccccc ctgtttttga
     2401 tacaaaacac gcctcacaga cggggaattt tgcttatcca cattaaactg caagggactt
     2461 caccataagg ttacaaccgt tcacgtcata aagcgccagc cgtcagccct acagggtgcg
     2521 atgtatcttt taaacgcctg tttatatctc ctttaaacta cttaattaca ttcatttaaa
     2581 aaaaaaacct gttcactgcc tgtcctgtgg acagacagat atgcacctcc caccgcaagc
     2641 ggcgggcccc aaccgaagct ggtttagtta caacatgcaa acataaaccc ctaaaaaacc
     2701 caagtccgac gtagaactga aaccacaacg cccctcctca taactaaaaa gcgccgccgc
     2761 ccccgcccga agggcgggaa cagagtcgct tttaattatg aatgttgtaa ctacatcatc
     2821 atcgctgtca gtcttctggc tggacgtatc gagtacacgc tcgtaagcgg ccctcacggc
     2881 ccgctaacgc ggagatacgc cccgacttcg ggtaaaccct agtcgggacc actccgaccg
     2941 cgcacagaag ctctgtcatg gctgaaaacg ggtatggctt ggcagggctg ggggtgggta
     3001 aggtgaaatc tgtcaatcag taccggctga cgccgggctt cggcggtttt actccggtat
     3061 cacatgtaac cacagcgtgc cgccttccat gccgctgacg cggcatcaga ataatacaag
     3121 cagcatttaa tgcatttgca aaagtgtgag taataaagac ttgttaagtg gtcgtttcag
     3181 ggatatcgtt aagactt
//
LOCUS       DADLSA010000267         3124 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.270, whole genome shotgun sequence.
ACCESSION   DADLSA010000267 DADLSA010000000
VERSION     DADLSA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.270"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            52..771
                     /locus_tag="JN691_004173"
     CDS             52..771
                     /locus_tag="JN691_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAY7245411.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCIYAVKSYPTYLADEPEWASRAE
                     KVAVRMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            782..2209
                     /locus_tag="JN691_004174"
     CDS             782..2209
                     /locus_tag="JN691_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HAY7245412.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            2202..2897
                     /locus_tag="JN691_004175"
     CDS             2202..2897
                     /locus_tag="JN691_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HAY7245413.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
BASE COUNT          799 a          675 c          890 g          760 t
ORIGIN      
        1 tgggggggaa acatcgggca taaatgggca tgaagtaatg gagtattagt tgtgaatgtc
       61 aatttctttg tcacctgtat tggtgacgcc ctgaaatcaa gaatggcacg agactccgtg
      121 ctgctactgg aaaaactcgg ctgtcgcgta aatttcccgg agaaacaggg atgctgcggt
      181 cagcctgcga tcaatagcgg ttatatcaaa gaagcgattc cagggatgaa aaatctgatc
      241 gccgcactgg aggataacga cgatcccatt atttcaccgg ctggctcttg catctatgcc
      301 gtaaaaagtt acccgacgta tctggcggat gaacctgaat gggcatcacg tgccgaaaag
      361 gttgccgtgc gtatgcagga tctcacctct tttattgtta ataaattagg ggtagtcgat
      421 gtaggtgcca gtttgcaagg gagagctgtg tatcacccat cttgtagcct ggcccgtaag
      481 ctgggagtga aggacgagcc acttacgctg ctgaaaaatg tgcgtggact ggagctgttg
      541 acctttgctg aacaggatac ctgctgcgga tttggcggca cgttctcggt caaaatggcc
      601 gaaatatccg gcgagatggt gaaagaaaag gttgtgcacc tgatggaagt ccgccctgag
      661 tatttaattg gtgctgacgt gagttgcctg ctgaacatca gtgggcgatt acaacgggaa
      721 gggcagaaag tcaaagtgat gcatattgct gaagtgttga tgagccgctg aggatataaa
      781 gatgtcgatc aaaaccagta atacagattt taagacacgc atccgtcagc aaattgaaga
      841 tccgatcatg cgcaaagcgg tggcaaacgc gcagcagcgt attggggcaa atcggcaaaa
      901 aatggtcgat gaattggggc actgggagga gtggcgcgat cgggccgccc agatacgtga
      961 tcatgttctg agtaatctcg acgcttatct gtaccagctc tcagaaaaag tgacgcaaaa
     1021 cggcggtcac gtctattttg caaaaaccaa agaagacgct acccgctaca ttttacaggt
     1081 tgcccaacgc aaaaatgccc ggaaggtggt gaaatctaaa tcgatggtga ccgaagagat
     1141 tggtgtcaat catgtgttgc aggatgctgg cattcaggtg attgaaaccg atctgggtga
     1201 atatattctc cagctggatc aagatccgcc atctcatgtt gtggtcccgg caattcataa
     1261 agatcgccat cagatccgtc gagtgctaca cgaacgtctg ggctatgagg ggccggaaac
     1321 gcctgaagcg atgaccttat tcatccggca aaaaatccgt gaagatttcc tcagtgctga
     1381 aataggtatt accggctgta atttcgcggt ggcagagacc ggttcggtat gcctggtgac
     1441 caatgaaggt aatgcgcgaa tgtgtaccac gctgcctaaa acgcatattg cagtgatggg
     1501 aatggagcgt attgccccca cgtttgccga ggtagatgta ttgatcacca tgctggcgcg
     1561 cagtgccgtt ggtgcacgtt tgacgggata caacacctgg ctgacagggc cgcgcgaagc
     1621 ggggcacgtt gatggtcctg aagagtttca tctggttatt gtcgataacg ggcgttctga
     1681 ggtgctggcc tctgaatttc gggatgtgct gcgctgtatt cgctgcgggg cttgtatgaa
     1741 tacttgtccg gcatatcgcc atattggcgg tcatggatat ggctctattt atccagggcc
     1801 aattggtgcg gtgatttctc cgctacttgg cggctataaa gattttaaag atttacccta
     1861 cgcctgctct ttatgcactg cttgtgacag cgtgtgtccg gtgcgtattc cgctgtcaaa
     1921 actgattttg cgtcatcgtc gggtgatggc tgaaaaaggg atcaccgcaa aagcagagca
     1981 acgggcgata aaaatgttcg cttatgccaa tagtcatcca ggattgtgga aagtcgggat
     2041 gatggccggc gctcatgcgg caagctggtt tatcaatggc ggcaaaacac cactcaaatt
     2101 tggcgcgatt agcgactgga tggaagcacg cgatcttcct gaagctgacg gagagagttt
     2161 ccgtagttgg tttaagaaac atcaggcgca ggagaaaaag aatggataat cggagcgaat
     2221 ttttgaataa cgttgctcag gcactgggtc gcccgctgcg acttgaaccg caagcagaag
     2281 atgcgccgct taacaactat gctaacgagc ggcttaccca acttaaccaa cagcagcgct
     2341 gtgacgcgtt tattcagttt gccagcgatg ttatgttgac gcgctgtgag ctgaccagcg
     2401 aggcgaaggc ggcagaagct gcaatacgtc tgtgtaaaga gctgggagat cagtcggtcg
     2461 tgattagcgg tgacacgagg ctggaggaat tggggattag tgaacgtttg cagcaggaat
     2521 gcaatgccgt tgtttgggat ccggcgaaag gtgccgagaa tatctcgcag gcagagcagg
     2581 ctaaagtggg tgtcgtgtat gctgaatatg gtttaaccga atcgggaggc gtggttcttt
     2641 tttccgccgc cgagcgcggg cgttcattga gcctgctccc ggaatcttct ctttttatcc
     2701 tgcgtaaaag cacaatcctg ccgcgtgtag cgcaactcgc agaaaaattg catcagaaag
     2761 cgcaggccgg tgaacgaatg ccgtcatgta ttaacatcat cagtggtccc agctcaacgg
     2821 cggatattga gcttatcaaa gtcgtcgggg ttcatggtcc ggtgaaagcg gtgtatctga
     2881 ttattgagga ttgctgagag agatatgaga tctgaacata aacctgaaga aggcatacga
     2941 ggttgaaaat agcgttaagg acaacctgac ttaaccccgt tccgtcatgg ttatgctgct
     3001 cttctgtaaa tatcctggca ttagtaatgc caggatattt acagtgaaaa agacaggcat
     3061 ccattcctga aaacgggttc ccttcccggt cattgtcact tcagctttat acaggcactc
     3121 tcta
//
LOCUS       DADLSA010000268         3113 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.271, whole genome shotgun sequence.
ACCESSION   DADLSA010000268 DADLSA010000000
VERSION     DADLSA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.271"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..345
                     /locus_tag="JN691_004176"
     CDS             <1..345
                     /locus_tag="JN691_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY7245414.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
     gene            335..1153
                     /locus_tag="JN691_004177"
     CDS             335..1153
                     /locus_tag="JN691_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7245415.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGANPRRRQWSITTDTGISYAT"
     gene            1143..2567
                     /gene="patD"
                     /locus_tag="JN691_004178"
                     /pseudo
     CDS             1143..2567
                     /gene="patD"
                     /locus_tag="JN691_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            2777..3113
                     /locus_tag="JN691_004179"
                     /pseudo
     CDS             2777..3113
                     /locus_tag="JN691_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
BASE COUNT          728 a          688 c          869 g          828 t
ORIGIN      
        1 atcctgcccg ttcaggcggc gcttgagcgt ttgccgccgt cattgttgca ggcgtcggct
       61 gatctcggcg cacgtccacg acaaaccttt cgctatgtgg tgctgccgct ggcaatcccg
      121 ggtattgccg ctggctctat ctttaccttc tcactcacac tgggcgattt tatcgtcccg
      181 cagctggttg gtcctccagg atattttatc ggcaatatgg tttattccca gcagggggcg
      241 attggcaata tgccgatggc ggcggcattc accctggtgc cgattattct catcgcactg
      301 tacctggcgt tcgtgaaacg tctgggagcg ttcgatgcac tctgaacgcg ctccgttttt
      361 cctcaaactg gcggcctggg gcggcgttgt tttcctacat tttcccatcc tgataatcgc
      421 cgcctatgcg tttaacactg aagatgcggc gtttagtttt ccaccgcagg gcctgacgct
      481 gcgctggttt agcgtggcag cacagcgtag tgatattctt gatgccgtga cgctgtcact
      541 taaagtggcg gcgctggcga cattaattgc gctggtgtta gggacgctgg cagctgccgc
      601 gctgtggaga cgagactttt tcggcaaaaa cgccatttcg ctgttattgc tgctgcccat
      661 tgcgctgccg ggcattgtca ctggtctggc gctattaacc gcctttaaaa ccatcaatct
      721 ggagccggga tttttcacca tcgtggtcgg tcatgcgact ttttgcgtag tggtggtgtt
      781 taacaatgtc atcgcccgtt ttcgccgcac ctcgtggagt ctggttgagg cgtcaatgga
      841 tcttggggcc aatggctggc aaaccttccg ctacgtagtg ttgccgaatc tcagttcggc
      901 gttactggca ggaggaatgc tggcgtttgc cttgtcgttc gatgaaatca tcgttacgac
      961 ctttacggca ggtcatgaac gaacgttacc gttgtggttg ctcaatcagc ttgggcgacc
     1021 gcgtgatgta ccggtaacca acgtggtggc actgctggtt atgttggtaa caaccttgcc
     1081 gatcctgggg gctaacccgc gaaggcgaca atggtcaata accactgata caggaataag
     1141 ctatgcaaca taagttactg attaactgag aactggttag cggcgaaggg gaaaaacagc
     1201 ctgtctataa tccggcaacg ggggacgttt tactggagat tgccgaggca tccgcagagc
     1261 aggtcgatgc tgctgtgcgt gcggcagatg cagcatttgc tgaatggggg caaaccacac
     1321 cgaaagcgcg tgcggaatgc ctgctgaaac tggcggatgt tatcgaagaa aatggtcagg
     1381 tttttgccga actggaatcc cgtaattgtg gcaaaccgct gcatagtgcg ttcaatgatg
     1441 aaatcccggc gattgtcgat gtttttcgct ttttcgcggg tgcggcgcgc tgtctgaatg
     1501 gtctggcggc aggtgaatat cttgaaggtc atacttcgat gatccgtcgc gatccgttgg
     1561 gggtcgtggc ttctatcgca ccgtggaatt atccgctgat gatggccgcg tggaaacttg
     1621 ctccggcgct ggcggcaggg aactgcgtag tgcttaaacc atcagaaatt accccgctga
     1681 ccgcgttgaa gttggcagag ctggcgaaag atatcttccc ggcaggcgtg attaacatac
     1741 tgtttggcag aggcaaaacg gtgggtgatc cgctgaccgg tcatcccaaa gtgcggatgg
     1801 tgtcgctgac gggctctatc gccaccggcg agcacatcat cagccatacc gcgtcgtcca
     1861 ttaagcgtac tcatatggaa cttggtggca aagcgccagt gattgttttt gatgatgcgg
     1921 atattgaagc agtggtcgaa ggtgtacgta catttggcta ttacaatgct ggacaggatt
     1981 gtactgcggc ttgtcggatc tacgcgcaaa aaggcattta cgatacgctg gtggaaaaac
     2041 tgggtgctgc ggtggcaacg ttaaaatctg gtgcgccgga tgacgagtct acggagcttg
     2101 gacctttaag ctcgctggcg catctcgaac gcgtcagcaa ggcagtagaa gaggcgaaag
     2161 cgacagggca catcaaagtg atcactggcg gtgaaaagcg caagggtaat ggctattact
     2221 atgcgccgac gctgctggct ggcgcattac aggacgatgc catcgtgcaa aaagaggtat
     2281 ttggtccagt agtgagtgtt acgcccttcg acaacgaaga acaggtggtg aactgggcga
     2341 atgacagcca gtacggactt gcatcttcgg tatggacgaa agatgtgggc agggcgcatc
     2401 gcgtcagcgc acggctgcaa tatggttgta cctgggtcaa tacccatttc atgctggtaa
     2461 gtgaaatgcc gcacggtggg cagaaacttt ctggttacgg caaggatatg tcactttatg
     2521 ggctggagga ttacaccgtc gtccgccacg tcatggttaa acattaagtt agatgttagt
     2581 ggctgttata ggacggccac tatcgtttta ggttctgagt ttaatggcgg ttaagtatta
     2641 gaatgattac attaataact agcgtaaaaa ttaggctaaa tgtttgatgg cgatttttca
     2701 tcctgtcaca gttgattagc ataaggcttt agagtatcgt tattaaacta ataatttata
     2761 agtagaagta ttaacaatga aagttcaatt gtacaaattc acggaagata aaaataaaac
     2821 ccttaccttc agatggacga aaaaacactt tgagttttgt atggataata aaatattttt
     2881 aaatcataag gggaagaagt cgtataaaga aaggaattta tttcttttct caaaaggtga
     2941 caagataaca attgaagata atgttattgc tgaagaatat tcaacaatgc ctgtgaaaaa
     3001 tttctccagt gtcggcgctt tttcatttcc gacctgccat ttttcaggga atatacgtat
     3061 aggtcgattt tgtagtattg aataatgggt ggaaatcatc ctttgaatcg ttt
//
LOCUS       DADLSA010000269         3097 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.272, whole genome shotgun sequence.
ACCESSION   DADLSA010000269 DADLSA010000000
VERSION     DADLSA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.272"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            25..801
                     /locus_tag="JN691_004180"
     CDS             25..801
                     /locus_tag="JN691_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HAY7245416.1"
                     /translation="MSAQSVNEIISKIGADAVALPENFRIHDLEKFNLNRFRFRGVLS
                     TASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEPGHADNTATLKLKKTAP
                     FSALLSVNGERHSQKSLAEWIEDWADYLVGFDANGDAIQATKAAAAVRKITIEANQTA
                     DFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKERPFKLRLSIITGDRPVL
                     VLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            929..1453
                     /gene="yfdR"
                     /locus_tag="JN691_004181"
     CDS             929..1453
                     /gene="yfdR"
                     /locus_tag="JN691_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY7245417.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPTPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPTVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLSPGHA
                     YGMFMERFKELTES"
     gene            1562..2428
                     /locus_tag="JN691_004182"
     CDS             1562..2428
                     /locus_tag="JN691_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245418.1"
                     /translation="MNQNPFSFYDFLGYLIPGGFFILLMYFCGLTFDLDIVIDLTELL
                     RGQSQIFGILNYGSIVIISYIAGHFISITSAFFIEKYMNEELQYPSEYLFNKLSDDKK
                     NVLTPSSDKQNEDTKRKIKYCIIKVMLFPIIPWDYATQKLCYSQSLPFQLANTTWLMI
                     KEGYEKKFRIDDKLLNIRTGLHDDLFRLAYHYVYEFSNQHQTKIQNYVALYGFCRNIC
                     LVFVITFWISFLTLIYRAIEGGAVSYISISTLLSFFFVYVFYVGFVKFYRRYTLEVLM
                     AFAVLQSKDAIN"
     gene            2469..2768
                     /locus_tag="JN691_004183"
     CDS             2469..2768
                     /locus_tag="JN691_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator protein PrtN"
                     /protein_id="HAY7245419.1"
                     /translation="MNTLFLLMAEFNTSNIELSAVSQKYFGMSPATAEAKAKAKAKAN
                     ACKLPVPTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTGKQKGKEHH"
BASE COUNT          956 a          619 c          618 g          904 t
ORIGIN      
        1 gcaattaatc aaatccatgc tcttatttct gctcagagtg ttaatgaaat tatcagtaag
       61 attggtgccg atgctgtggc attgcctgag aatttccgca ttcatgatct ggaaaaattt
      121 aatttaaatc gcttccgttt ccgtggtgtg ctttccactg ccagcatcga tgactttacc
      181 cgttattcta aagatcttgc agatgaaggc acccgctgct ttatcgatgc cgataatatg
      241 cgagccgtca gtgtgcttaa cctgggtact attgatgaac caggtcacgc agataacacc
      301 gccactctca aactgaaaaa gacagcaccg ttctctgctc tgttgtctgt taatggcgag
      361 cgtcattccc agaagtcact ggcagaatgg attgaagact gggccgacta ccttgtgggc
      421 tttgatgcta atggtgacgc tattcaggca acaaaagcgg ctgcggctgt ccgtaaaatc
      481 acgattgaag caaaccagac cgctgatttt gaagataatg acttcagcgg caaacgctcc
      541 ctgatggagt ctgtcgaagc gaaaaccaaa gatattatgc cagtggcatt tgaatttaaa
      601 tgcgttccgt ttgaaggtct gaaagaacgt ccatttaaat tacgcctcag cattatcact
      661 ggcgatcgtc ctgtactggt tctgcgcatt attcagctgg aggcggtgca ggaagaaatg
      721 gctaacgaat ttcgtgatct gcttgttgag aaattcaaag acagcaaagt agaaaccttt
      781 attggtactt tcaccgcctg atttcattac tgcaaatgcc cctgcggggg catttatgga
      841 aacgtaattg actcaataat cgccggatgg tgagggcttc cttttaccag aattcagcgt
      901 ggtgcagcac atatacgcgg agaacaaaat gtcatttatt aaaacttttt ccgggaagca
      961 tttttattat gacaggataa ataaagacga catcgtgatt aacgatatcg cggtttccct
     1021 ttcaaatatc tgtcgctttg ccggtcatct ttcacacttc tacagtgtcg cccaacatgc
     1081 ggtgctttgc agccagctgg tgccgcagga atttgctttt gaagcgttaa tgcatgatgc
     1141 aacagaagcg tattgccagg acatccccac gccgctgaaa cgccttcttc ctgactataa
     1201 acggatggaa gaaaaaatag acgccgtaat ccgtgagaaa tacgggttac ccacggttat
     1261 gagcacgcct gtgaaatatg ccgatctcat catgctggca accgaacgcc gcgatctcgg
     1321 gcttgatgat ggctctttct ggcctgtact ggaaggtatc ccggcaacag agatgttcaa
     1381 agttattcca ctgtcgccag gccatgccta tgggatgttt atggaacgct ttaaagagtt
     1441 aactgagtcg taaaaatcag cacgtacgaa ttcaaactct gccataaaag aacatataag
     1501 taatttatta acatatagat ataggttata ttacaaattg aaaaattatt ggagagcaac
     1561 aatgaatcaa aatccattct cattctatga ctttcttgga tatttaatac caggtggttt
     1621 ttttatccta ttaatgtatt tctgtggttt gacattcgat ctagatattg ttattgactt
     1681 aactgaattg ctcagaggtc aaagtcaaat ctttggcatt ttgaactatg gttcaatagt
     1741 aatcatatct tacatagctg gacactttat atctatcaca tccgcatttt ttattgaaaa
     1801 atatatgaac gaagaattgc aatatccgtc agaatatcta tttaataagt tatcagatga
     1861 caaaaaaaac gtcttaacac catcaagtga caaacagaac gaagacacaa agagaaaaat
     1921 aaaatactgc ataatcaaag taatgttatt cccgataata ccatgggatt acgccactca
     1981 aaaactatgt tattcacaat cattgccatt tcaattagct aataccacat ggctaatgat
     2041 caaagaaggt tatgaaaaaa aattcagaat agacgataaa ttattaaaca tcaggacagg
     2101 tcttcatgat gatcttttca gattggctta tcattacgtc tatgaatttt ctaatcaaca
     2161 tcaaacaaaa attcaaaact atgttgcatt atatggtttt tgcagaaata tttgtttagt
     2221 atttgtaatt acattttgga tatcatttct aacattaatt tatcgtgcta tagaaggtgg
     2281 ggcggtgtct tatatttcta tttcgacgct acttagcttt ttcttcgtct atgtttttta
     2341 tgttggattc gttaagtttt atagacgata tactttagag gtattaatgg catttgctgt
     2401 acttcagagc aaagatgcta ttaattaata tattaccctc gggtgcagcc gggataatgg
     2461 agaaatttat gaacaccttg tttttactga tggctgaatt caatacctca aacattgaac
     2521 tctcagcagt tagccaaaag tactttggta tgagtccagc cacagcagaa gcaaaagcaa
     2581 aagcaaaagc aaaagcaaac gcttgtaagt tgcccgttcc aacatatcgc atcggcacat
     2641 cacaaaaagc aaaacgttgc atcaatattc aggatcttgc ggaatacata gacaaaagac
     2701 gagaagaagg acgtatcgag tgggaacagg tcagaacagg caaacagaag ggcaaagaac
     2761 atcactaaag aaaaaacccg cctaaaggcg ggttttcaaa aagcaccagc tatgatcatg
     2821 ctgctttgcg acgacgaagc ttaccctgct gctctttacc agagacagta gcgtgagtga
     2881 acgcattagg agcagccttc atcagaactt caaagcagcc cccatacctg cgaatgcttt
     2941 cattgtgtcg aacttaagct gtggcttggt tgctttttga tcttccatag aaaactccag
     3001 aagttatacc gaaacaattc ctgttgttta ctcatcatca atagatgata cgcaatattt
     3061 atttttaaat ttaaggttct ttggcgtaac ttcatca
//
LOCUS       DADLSA010000270         2966 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.273, whole genome shotgun sequence.
ACCESSION   DADLSA010000270 DADLSA010000000
VERSION     DADLSA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.273"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..298)
                     /locus_tag="JN691_004184"
     CDS             complement(<1..298)
                     /locus_tag="JN691_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245420.1"
                     /translation="MLEIVLSPVKAQQFTVTLGAQVCTIRLNQRTTGMYIDIIVNGEP
                     CLYGVLCLNNNRIVRYGYLPFQGDLFFTDTEGNSDPDWRGLGSRYRLYWLLPEEL"
     gene            complement(298..1008)
                     /locus_tag="JN691_004185"
     CDS             complement(298..1008)
                     /locus_tag="JN691_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245421.1"
                     /translation="MSFSFDNISLNNFSLSESNVLSAVRGGGVLGLINSVLAPSFGIY
                     YARNDPAGAHQKGGKPFSPDSFVVVEVGAEASVSTVPVEQGAYTTFNKIQRSPELHVT
                     FTVEGWTAFSGSVPNLTNFSTTSRTNVLETLERMRTTAGLYDIETPDKTWTSCDLVKY
                     DYRMRSNNGPTLLTVTAVFQTVMNTGEVSVGSTDKSSPTDNDKAKGAASVKTQPVTAS
                     VTQPSDADRRSATNRGIT"
     gene            complement(1005..1993)
                     /locus_tag="JN691_004186"
                     /pseudo
     CDS             complement(1005..1993)
                     /locus_tag="JN691_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179335.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
BASE COUNT          679 a          877 c          715 g          695 t
ORIGIN      
        1 tcagttcctc aggtaacagc cagtagagcc ggtaccggga tccgagcccc cgccagtcag
       61 ggtcactatt cccctccgtg tcggtaaaaa acaggtcccc ctgaaacggc aggtatccgt
      121 accggacaat ccggttatta ttcagacaca gcacgccata caggcacggt tcaccattaa
      181 caatgatatc gatatacatc cccgtagtac gctgattcag gcgaatggtg cagacctgag
      241 cgcccagtgt caccgtaaac tgctgggctt taacgggaga taaaacaatt tccagcatca
      301 ggtgatcccc ctgttcgtgg cgcttcgtct gtcagcgtct gacggttgtg tcaccgacgc
      361 cgtaactggc tgcgttttaa ccgatgcggc cccttttgct ttatcgttgt ccgtgggaga
      421 cgacttatcc gtacttccca ctgacacctc tcctgtattc attacggtct gaaataccgc
      481 agtaaccgtc agtaaggtcg gaccattatt acttcgcatt cggtagtcat atttcacaag
      541 gtcgcaggat gtccatgtct tgtccggcgt ctcaatatcg tagagtcctg ccgtggtacg
      601 catcctttca agcgtttcca gcacattcgt ccgcgaggtg gtggaaaaat ttgtcaggtt
      661 cgggacggat ccggaaaagg ccgtccaccc ctctacagtg aaagtcacat gcagttccgg
      721 cgatctctgg attttattaa aggtggtata agccccctgc tccacgggga cggtggaaac
      781 agaagcctcc gcgcccacct caacgacaac aaaagaatcc ggggagaaag gtttgccacc
      841 cttctgatgg gcacctgccg gatcattccg tgcgtaataa ataccgaatg acggtgccag
      901 tacactgtta atgagtccca ggacaccgcc gccacgaact gcactcagta cgttactttc
      961 actgagcgaa aagttattca gggaaatatt atcgaaagaa aaactcatcc tgttaccccg
     1021 ctggaataaa ccgacacaag cgccgaattc gtgatacgcc gacgtgcgtc atcggtaata
     1081 cccttcacat tgtccgacgt tgtggtgaca ttcagtgtcc cgatatgcgt ggtttccgtt
     1141 acggtggact gcgatacagg cgcaggatgg cgcgaccgta tggccattgc cgcacccgga
     1201 taaggcaggt tcgccagcac ccgcggaata tagttgcggg tctcctccgg agcagcagcc
     1261 agtcccttac gctgaacatt tccctctccc cagttatatg ccgccagagc cttagccaga
     1321 tcgccatgaa aaaaacgtat caggccacca agttttctcg cggcggcatc agcggctttt
     1381 tcgggatcaa aggcatcgtt ccccctcaga ccaaattcac tggccgtctt cggcatgaac
     1441 tgaaacagtc ccatcgcacc agcaggtgag acggcaaact gattaccacg ggattcggtg
     1501 atcgcaacgc tgcgcagcag tccgggcggc aggttatatt tttcctccag ttgagacagt
     1561 ttcggttgca gccagcctaa cagggcttcg tcggtcgctg gcggttttgc atggcattac
     1621 cgagtttttc ctgcgtcgca cgcataccct gtagccagga ttcaccgaag gctgcttccc
     1681 tcccggttgc ctgagaagcc tgagtatcca gcatgccctg ctgccatact gtgggtgatt
     1741 gtgcccgggt gatgttgcca gacttttctc cggcatccag ttttgtctga tacgtctcca
     1801 tctctttctt attgaaaaag aaagtcccgt ctgaagccca gaaaaaacca tgtgaatcca
     1861 gccagtcctt attttccctg ccaacaatgg atgtcagtaa tccgtcaaca accgggtaaa
     1921 gcgccgttat cgcaaaaaga aggcctccgg ggccactgag ggcttttgtc agcccccgca
     1981 cccatgacgc cactttcagc ccgatcagcg taataatgac attctgccag ccccccagtt
     2041 ctccggcagc cttattcgcc agggaggcca ctgactcaac gtcattcaga aatgtggtga
     2101 taaacctgtt cacttcctcc ggatgttgct gcatccagtc accgagtgtc tccagccagt
     2161 ggctgagctt cagcgcgtac ggcatcaacg cggtgctaat cgtcatcccg atgccggacc
     2221 aggtctgatc gagctcaaca agctcttttc gtagcttacg ggccagcgcc atacgccccg
     2281 gcgtaacctg cgaacgggaa gtaaattcgt ccacatcttt cagcgcatga ccggagccaa
     2341 gaaactgctg tccggcataa ctgaagccca gcgcgttgcc gtaggcagtt tgttcggact
     2401 ttgtcagtcg cggaaaaata accgccagct tgcgcatgat ggcttcagtg ctgtcagtat
     2461 ttaaatcaac actaatgccc gccctggctg caacctgaaa caaatcctgt aacacaggat
     2521 caaaggacct tccggctctg aaggccgctt ttgcatttgc aagccgggaa aatgccccag
     2581 acatttcccc ttcatcaaca ttatttgccc gcgcagcccg taaccatccg tccagatttc
     2641 tggccttcat gccaaaagca tcagattcca cggacagacg gttcagatca cgggcaaaac
     2701 ctgttaccag gttcttcagc ccccccagcg tcagactgac acccgcaaga gccagaatct
     2761 cgctacggat ccctgaaaaa aaacgggacg cccgtttccc ggcatcttcc attcgacggg
     2821 ccgttttatc agcatctttt ccggtttttt ccagtgattc actggttttc ttctgtccct
     2881 gactgaatgt gtcagcgaca ccttccatca ccgtcgtgag ccggttaagg gcagcatgta
     2941 ctgcctgttc cccggcagta aagtcc
//
LOCUS       DADLSA010000271         2936 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.274, whole genome shotgun sequence.
ACCESSION   DADLSA010000271 DADLSA010000000
VERSION     DADLSA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.274"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            246..1859
                     /locus_tag="JN691_004187"
     CDS             246..1859
                     /locus_tag="JN691_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7245422.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYFAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1910..2936)
                     /gene="ynaI"
                     /locus_tag="JN691_004188"
                     /pseudo
     CDS             complement(1910..2936)
                     /gene="ynaI"
                     /locus_tag="JN691_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
BASE COUNT          850 a          645 c          685 g          756 t
ORIGIN      
        1 gcgacagatg ctgattatga taccgaaaac tggtttgaac gtgcgaagcc cgaacgtagt
       61 gttcggtgtt ctatgtgctt tgccgcattt tggcgtctat tatttacact aaatctgatt
      121 tgatatattg atacttaaaa catttgatgc ttcctttgtc acttttttga tggaagttgt
      181 ttgcatttct ttaaggcgaa acaaataatt acgcatcaat tttaatgtcg gttagaggga
      241 aacttatgaa gcactctgtt tcagttacgt gttgtgcgct gttggtcagc agcatttctc
      301 tttcgtatgc tgcagaagtt ccgagcggca cagtactggc agagaagcag gagctggtgc
      361 gccacattaa agatgagcct gcgtcgctgg atcccgctaa agccgtgggc ctgccagaga
      421 ttcaggtcat tcgcgatctg tttgaaggtc tggtgaatca gaacgaaaaa ggggagattg
      481 tccccggcgt cgcgactcaa tggaaaagta atgacaaccg tatctggact tttaccctgc
      541 gcgataacgc aaaatgggcg gatggcacac cggtaacggc gcaagatttt gtctacagct
      601 ggcaacgtct ggtggaccca aaaacattgt cgccatttgc atggtttgcc gcgctggcgg
      661 gaatcaacaa cgcacaggcg attattgatg gtaaagctac gcctgatcag cttggcgtca
      721 ccgcagttga tgcccatact ttgaaaattc agcttgataa accgttgccg tggtttgtga
      781 atttaaccgc taactttgcc ttcttcccgg tgcaaaaagc caacgtagaa agcggtaaag
      841 agtggacgaa acccggaaat ctgatcggca atggcgctta tgttcttaaa gagcgcgtag
      901 tcaatgaaaa actggtcgtg gtaccgaata cccattattg ggataacgcc aaaacggtgc
      961 tgcaaaaagt gaccttcctg ccaattaatc aggaatccgc agccactaag cgttacttcg
     1021 cgggggatat tgatatcacc gaatccttcc cgaaaaatat gtatcagaag ctgttgaagg
     1081 atattccggg gcaggtttat acgccaccgc agctcgggac ctattattat gcctttaaca
     1141 cgcaaaaagg gccgacggca gatcagcgcg ttcgcctggc attaagtatg acgatagatc
     1201 gccgcctgat gaccgaaaaa gtgttgggga cgggcgaaaa gccagcgtgg cattttatac
     1261 cagatgttac cgccggattt acgccggaac cttcgccgtt tgaacaaatg agtcaggaag
     1321 aactgaatgc gcaggcaaaa actttgttga gcgcagctgg ttatggtccg caaaaaccgc
     1381 tgaagctgac gcttttgtat aacacttcag aaaaccatca aaaaattgcg attgctgtag
     1441 catcgatgtg gaaaaagaac cttggcgtag atgttaaatt gcaaaatcag gaatggaaaa
     1501 cctatatcga tagccgtaac accggcaatt ttgatgttat ccgcgcctca tgggtgggtg
     1561 attataatga accctccact ttcctgacat tattaacgtc aatgcattca ggaaatattt
     1621 cacgctttaa caatccggca tacgacaaag ttctggccca ggcatcgacg gaaaataccg
     1681 ttaaagctcg taatgccgat tacaacgcgg cagaaaaaat cctcatggag caagcaccga
     1741 ttgcaccaat ttatcaatat accaatggac gattaatcaa accgtggctg aaaggttatc
     1801 ccattaataa ccctgaagat gtagcgtata gtcggactat gtatattgtg aagcattgat
     1861 gtgatgggaa ctggcgttac ccttgtggat aacgccagtg atgtctgatt tagcgaccct
     1921 gttccggcgg tgtaatgtta tccatataca gcgtctggct ggggaaggca aagtccgcgc
     1981 cgtgtgactg tacaatatcg ataatcttca aataaacgtc ttgctgtgca gcaagccact
     2041 cagcccatac cgtggttttg gtaaagcaat aaaccataat attcaatgac gagtcagcaa
     2101 actggttgaa ataaaccagt aaggtttgtc gctggtcgat ggccgggtga tttttcagca
     2161 tctcacgtac ggcttcgaca ataacgccca cttttgccgc atcctcataa cgtaaaccaa
     2221 tggtcgtggt aatgcggcgg ttggtcattc gtcctgggtt ttctacgctg atcgacgaaa
     2281 acagcgagtt cggtacgtac aatggacgat tatcaaaggt cgtaattttg gtaattcgcc
     2341 agccaatttc cgctactgta ccttcgatat ttctgtccgg tgaacggatc cagtcgccaa
     2401 tactgaaagg acggtcgaaa tagagcatga tcccggaaaa gaaattactc agaatatctt
     2461 taccggccat accgacagcc agaccaccaa taccaccaaa ggtcagcaag ccagaaaggc
     2521 tcatgccgaa atgttcgcca taaagcagaa caagcactac aataatggtg attttgataa
     2581 tgcgcgacat aatccgcgca ctggtgatat cgcgaccttt tttaatctgc tgtttttcaa
     2641 actgattaat cagcagaaat agcttaatcg tcagaataac cgcaatcagg gacgtacaga
     2701 taaaatcgat aacgcctggg gtgataaatt tgagtttata gttttctata acataattaa
     2761 tgatgctacc aacagcactg ataattatgg tttagattaa aaattgcacc gcatggaata
     2821 aaaatccttt tcttttacga tttccacggc gaaaccaaaa gctcatcaga atcaatgctg
     2881 cgcagctacc gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagcg
//
LOCUS       DADLSA010000272         2918 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.275, whole genome shotgun sequence.
ACCESSION   DADLSA010000272 DADLSA010000000
VERSION     DADLSA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.275"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..178
                     /locus_tag="JN691_004189"
     CDS             <1..178
                     /locus_tag="JN691_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245423.1"
                     /translation="GASLSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGK
                     INQTTTKMLFMCRE"
     gene            complement(286..687)
                     /locus_tag="JN691_004190"
     CDS             complement(286..687)
                     /locus_tag="JN691_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAY7245424.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            929..1222
                     /locus_tag="JN691_004191"
     CDS             929..1222
                     /locus_tag="JN691_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245425.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            1294..1953
                     /locus_tag="JN691_004192"
     CDS             1294..1953
                     /locus_tag="JN691_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAY7245426.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            2030..2491
                     /locus_tag="JN691_004193"
     CDS             2030..2491
                     /locus_tag="JN691_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAY7245427.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKDLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2698..>2918)
                     /locus_tag="JN691_004194"
     CDS             complement(2698..>2918)
                     /locus_tag="JN691_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin E"
                     /protein_id="HAY7245428.1"
                     /translation="ANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLK
                     EAAKKMINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT          758 a          607 c          721 g          832 t
ORIGIN      
        1 aggggccagt ctatcattac aaggctacga tcccagcgaa tttacattcg ctaacggtgt
       61 attttgcgat gttaaagaaa aattatgtcg tgatgatcgt tattttggtg tagatggtaa
      121 gcggagtgga aaaatcaatc aaaccaccac aaagatgtta tttatgtgtc gtgagtgatg
      181 ccttgtttac attatgttct ctgctattcg tcattaacct cttgagcgac agagaggagt
      241 ggggcgttaa cgtctatgac ggggatagtc cccgtcggtt gacatttact taatttgaat
      301 atcgacgtta tattttttag ttttgttttt acgggcgtcg tttcgagttt gcagcactcg
      361 cagctcatat ttgccagaag cagggagtga gtattgtccg tgactatcga gttctgggga
      421 atatctggat aaatcaacag aatcatcgat tcctggcccg aagagataag tatcagcgcc
      481 ttcattagag atactcacat gcactttctg accttttttg gcatagaagg tatatgtatc
      541 gtaatcgtat ccctttattt cgccggagta ctgagcgctg ctatggcctt tgctgaactc
      601 aacattgaca ttcttacccg cagcaaagct cgtggaggtt agcagtgcaa gcagcaatac
      661 agccttactg atgctcttga ttttcattgg tagtctcctg ttcagaatga gtacgatgaa
      721 ctgttataat ataacaatcc cttacgggag aattccagtg gcttttctgc gttcggtaaa
      781 atcccggcgc ggcatttttt gccgggggga ttacttataa aggggcatgg actcaacaaa
      841 ccgcagaaat ctacagctgg ctggcttgtg gcgcgggttt cattgatgtt agcttatgcc
      901 aaaacctggc atcttaaaga gtaaaagtat gttttgtgtg atttatcgaa gcagcaagcg
      961 tgaccagacc tatttatatg tcgaaaaaaa agacgatttt tcgcgtgttc ctgaggaact
     1021 gatgaaaggt tttggtcagc ctcagttagc gatgattctg ccgttggatg ggcggaagaa
     1081 actggtcaat gctgatattg aaaaggttaa actggcatta accgagcaag gttactattt
     1141 gcagttaccg ccaccacccg aagatttgct gaagcaacat ctttccgtca tggggcagaa
     1201 aacagacgac actaacaaat aaccgatatc cggcggtggc attatctttg tcggcgcggg
     1261 ttttcatatc cacgataagg tgaggggaac gttatgtatc aacatcacaa ctggcaaggt
     1321 gcgctgctgg attatccggt gagtaaagta gtctgtgttg gcagtaacta tgccaaacat
     1381 attaaagaga tgggcagcgc agtgcccgaa gagccagtgc tgtttattaa accagaaacg
     1441 gcactgtgcg atctgcggca gccattggcg atcccatccg atttcggttc agttcatcat
     1501 gaagtcgaac tggcggtgtt gattggcgcg acgctgcgtc aggcaacgga agagcatgtc
     1561 cgcaaagcca ttgccggtta cggcgtggcg cttgatctga cattgcgtga tgttcaggga
     1621 aaaatgaaga aagccgggca gccgtgggaa aaggctaaag cgtttgataa ctcttgtccg
     1681 ctttccgggt ttatccccgc ggcggaattt accggcgatc cacaaaatac aacgctgggc
     1741 ctgagcgtaa acggcgaaca acgccagcaa ggtacgactg cggacatgat ccataaaatc
     1801 gttccgttga tcgcttatat gagcaagttt tttaccctca aggccggtga cgttgtgttg
     1861 acaggcacgc ctgatggcgt cggcccgttg caaagcggtg atgagctgac agtcactttc
     1921 gatgggcatt ctttgacaac tcgcgttttg taatactttt tgccgccaga aagcggcggc
     1981 aaaacttgca tcgctgtgcc agactggtta taaggtgcgt tttaacgtaa tggcggaaca
     2041 cctgatgagc gatgtacctt tctggcaaag taaaaccctg gacgaaatga gcgatgcgga
     2101 atgggagtcg ttgtgtgatg gttgcggtca gtgttgcctg cataaactga tggatgaaga
     2161 caccgacgaa atctacttca ctaacgtcgc ctgtcgccag ctcaatatta aaacctgtca
     2221 atgtcggaac tacgaacgtc gtttcgagtt tgaacccgac tgcattaaat taacccgtga
     2281 aaatctgcca acattcgaat ggctgccaat gacctgcgct tatcggttgc tggcggaagg
     2341 taaagattta cctgcgtggc atccgctact tactggttcg aaagcggcaa tgcatggtga
     2401 acgtatctct gtgcgtcata tcgcagtgaa agaatcagaa gtgattgact ggcaggatca
     2461 tatcttaaat aaacctgact gggcgcagtg ataaatttaa tagatgctga tgggggcttt
     2521 cttaatatac gtctggatgc tcgccatttc gtagccctgt ttttgaaagc gctctgtcat
     2581 aatgagagtt acccgatacc ttattatcaa caggtaactc tcaatcttta aaaaacgtct
     2641 gtgtttttaa atgtgataaa ccttatacct tgagtacatg gagagaataa tcgcttatca
     2701 gacttcaggt acctcaaaga gtgtcttttt accgtgtctt ttctgatact cattacaggt
     2761 gttaatcatt tttttggccg cttcttttag caaagaaagc attaaatcat cataatcaac
     2821 atagaatctg gttgtttcag tttccgtttt tatctccccg atggcggcta tttcggtggt
     2881 taatttcaat ttggcggcat cgatatcttt attcgctt
//
LOCUS       DADLSA010000273         2909 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.276, whole genome shotgun sequence.
ACCESSION   DADLSA010000273 DADLSA010000000
VERSION     DADLSA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.276"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..451)
                     /locus_tag="JN691_004195"
     CDS             complement(<1..451)
                     /locus_tag="JN691_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7245429.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPL"
     gene            complement(467..1363)
                     /locus_tag="JN691_004196"
                     /pseudo
     CDS             complement(467..1363)
                     /locus_tag="JN691_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(1363..1968)
                     /locus_tag="JN691_004197"
     CDS             complement(1363..1968)
                     /locus_tag="JN691_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY7245430.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(2139..>2909)
                     /locus_tag="JN691_004198"
     CDS             complement(2139..>2909)
                     /locus_tag="JN691_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707671.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HAY7245431.1"
                     /translation="ALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNP
                     KADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPAVV
                     LKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIW
                     WSTLAKSNLTFFKFGALSNRHTPPAALAAEIDPEWWIVAMNNPRFPVDVLKARLKRDP
                     LLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT          882 a          730 c          601 g          696 t
ORIGIN      
        1 ccagcggcac atacaattta accttaaagg ccggagaagc cagcaccagc ccacgaattg
       61 caggtgcata atcatgaacc catgtggcaa caagcactgc gccgacgctt tgcgcgatca
      121 ctaccacctc ttccagtccg acctggctgt cgctggcagc aaaacggaca aattcatcga
      181 catcctgcac tgagcgcata agagacgggc tataaccacg cggccccgaa ttttttccat
      241 gcccccgggc atcccaggca taaaaagcag tatcaggcat cgccagttca tcaacgagat
      301 gttgtagacg accagaatgt tcatgcccgc gatgaaataa gacgatgacc tttttcgccc
      361 ctggctgtaa agtgggccag tgccgataat acaacgctgt attatcactg gtggtaaaaa
      421 aatgttcccc agggatgcgt gaattttcca ttttctctat tttccttcag taacagcagt
      481 agcgaataaa ataaaaaaat accggagcgg tgaaaattaa tgagtcaatc ctgtcaagga
      541 gtccgccatg tcctagtaat agttttccac tatctttaac accaatatct cgtttgatgg
      601 ctgacatcac tacatcgccg caaaaaccac taataccaat taacaaaccc gctaataatg
      661 cctgtaatgt atttagcggt gtcagcaacg gtccaataat cagtgacgca atcatggtgg
      721 tgattacgcc ccccagcaga ccttccagcg tttttcccgg gctgactttg gggaccactt
      781 ttcttctgcc gcaggatttc ccccataaat actgtgcaat atcgtttgac tcggttaacg
      841 ccaaaagaaa taacaccagt aacgccccgc cctggatatt tatggttggc aacattaata
      901 gccaggcggc atgactcaag gcaaaaacgg tcattatcca tccccaaaaa atggcgctca
      961 cggtatgctg aaaaccggag gggtcaccca caaaaactcg ccaggtggct aatatcagaa
     1021 acccagtcaa aggtataaat aacagaaaca gctcaaagca attaaaaccg ataagcaaat
     1081 agttaagagg aataacccaa tataaccaac gcgaaaaatg tacggatata agcgtacaat
     1141 attctttcaa tgccagaaaa cttatcagcg taaaaaacgt caacgtcatc cagcgtgggc
     1201 ctgaaatcac cagtgaaaaa caaatgacga tgccccacta ggtacgaata cgtaatgtaa
     1261 cttcatcccc ttttctctca ggtaaccgcc atagcagaaa acggtttatc agcgttgcta
     1321 aaattaacaa cgcgaaaagt gttgccagag atttttccag cattatcttt cagcgctcat
     1381 aagaagcaca ctccgacaac gattaatcgc agtacagata agcagaattg aagcaatgct
     1441 ccataaaaga ttattccatt gcatccactg tggataaatg gcaacggcca gaccccacag
     1501 accaaatatt aacgcgcgat cacttttgcc aaatggaccg gcataactgc gaacaccgtt
     1561 aatcgtctgc gcgagtaaac cgcaaaactc ggtcaatatg gtgcaaaata acatgagtat
     1621 cacgagtgat gcgttacttt ccggtaaaaa taaaaacggt aaatagagcg caatatcgga
     1681 aataacatcg ccagtctcat tcaaaatcgc ccccagacgt gtttgctgat tgcactcacg
     1741 cgccaacatg ccatccagcg cattgagcgc catacggata aaaagcacga tgggcaatag
     1801 caaaaagagg atgggttgtg ccaccaacac caacagcaat ccggtaaaaa gagaaagcgc
     1861 cagtgccgta agagtgatgt gattcgctgt aacgtggtgc ttataaagcc aaaacatcgt
     1921 cggccttaac agcgactgaa agagcggttt tatctggtag agcgtcatta tcaatccttg
     1981 ataattcggt ttgcgctatg gtgcatcaat tcccctgacg gagatatcgc aacacgccgc
     2041 ttcattaaaa attcagaata tatcaaggta ttgggggctc actgtcagga atgattatcc
     2101 ccaataaatc aagtagttaa aaattattac gctgttattc aatacaactc atcaagtttc
     2161 ctcatcgctt gttcccgtat tgcgcgcgtc ttaccgttaa gcgccagctg gcggactaaa
     2221 tccagttcag ggttaacaag ctctaacgcc agcaaaggat cacgtttcag cctcgccttc
     2281 aatacatcaa ctggaaagcg gggattattc atcgccacaa tccaccactc gggatcaatt
     2341 tctgccgcca gtgccgcagg gggggtgtgg cggttgctaa gtgcgccgaa cttgaaaaat
     2401 gtgagattgc ttttcgccag tgtactccac catatacgat ccggttgttt tgccagtaca
     2461 tccagttgtg ctaacgcatc ggggtcgagt gcataataaa cacgtaatgc ttcatcaacc
     2521 tgcgcacggg agctattttc ctgcctcagg aaaaacttcg cccaaatatc attgcgtggc
     2581 gtgttttgct gtaattcctt gagaacaaca gcgggtaaat tatcccaact aactaatgca
     2641 ctctctaaaa agtcccagtc ttccaaacgt atcagggcgg cggcatttaa tagcatctgg
     2701 tcatcaactt cgccgtggct aatcagctca gacaaaatca tatcagcgct ggtttcatat
     2761 gcatctgaat ctgctttcgg attatttttc cgatgcgata aagcacgttg ccaaattttt
     2821 tgtttcatcg acgtgctcag atactgattt ttcgccagat tactccaccc ctcaggatcg
     2881 ttgaaacgtg tcaataaccg ttgtaaagc
//
LOCUS       DADLSA010000274         2848 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.277, whole genome shotgun sequence.
ACCESSION   DADLSA010000274 DADLSA010000000
VERSION     DADLSA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.277"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(254..1051)
                     /locus_tag="JN691_004199"
     CDS             complement(254..1051)
                     /locus_tag="JN691_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7245432.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGISFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1061..1612)
                     /gene="ybcL"
                     /locus_tag="JN691_004200"
     CDS             complement(1061..1612)
                     /gene="ybcL"
                     /locus_tag="JN691_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HAY7245433.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKISVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(1781..2113)
                     /gene="emrE"
                     /locus_tag="JN691_004201"
     CDS             complement(1781..2113)
                     /gene="emrE"
                     /locus_tag="JN691_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HAY7245434.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2458..2772)
                     /gene="fliE"
                     /locus_tag="JN691_004202"
     CDS             complement(2458..2772)
                     /gene="fliE"
                     /locus_tag="JN691_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAY7245435.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
BASE COUNT          840 a          637 c          552 g          819 t
ORIGIN      
        1 cctttaatta tcccctcaac ctcttgtaaa tttcccactc ttgagctcca catagtccaa
       61 acgccggatg caaaaaaaat cacacgtgca tctctgaaat tgtgatggag gaagtgagta
      121 tcaattatat gtgcttataa acgttcccga ttggcacaga agtcaccgga ttgttcaggc
      181 tccaataggt tgagtatgtc gtatttattt tccaaaataa aaatcgatgc tgttgctcct
      241 taataaaata cttttactct ctggacgctt ttcttagctc tcttggtgtt atcttaaaac
      301 gattatggaa cctttcagta aaacgagaag gacacttgta accattttcc ctggcaatct
      361 cgctgatggg ttttactgtc gtttgtatag cagacaacgc attatttaac ctcacgtcgt
      421 ccaatatact ttgaaaactt attccctcgc ttgccagacg gcgatgcaat gtggaaacag
      481 aaatgtagag atatctcgct accttgtttg ctgtccattt tgttccaggt tcggatagca
      541 ttaggttata acaacgactt attaatgatt gtttactatc tgataaaagt aaacaattaa
      601 catgatgtgc tcctaatgaa agtagaacgc ccattgctaa gtgttcctga atttgcgttg
      661 agaagcctcg ggaaatagat gtttttagtt gctcccaaca atatattaac tcggggttct
      721 gaggtaagca gaaacctgtc gtgttacgaa tttgatcagt tatcgcataa aatttttgaa
      781 acttttcaat tagatcaatg ggataataaa gcatttctgc aagataaaga cctgcttctg
      841 gataattcac aatataaaat tcatatccac agggaaataa tattatctga ttattatcaa
      901 cagttagact atgcgtctcc caattgataa ctttttttcc ctgacggata cgacataaag
      961 ctggcataag aggcttaacc ctgtgaatct catgatgttt atgcatccgt atttcttcaa
     1021 tctttaggtt agacttatct tttgccagca tactcttacc ctactttatc tcataaactg
     1081 gtgttatctc agcggttgca attttattgg cattaagcat ataaccaact aacgctccgc
     1141 tggaattaga atccacagaa atcttatcag tttttagagc ccatacttta aactggtaat
     1201 gatggggttt atctcctttg ggaggacatg ctccaccaaa tcctgcataa ccaaaatcat
     1261 ttcggccttg aacagcacca gtcggaagtt ttgtaccatc acgtcttcct gcatcagtgg
     1321 gcaaatatgt tacagttgct ggaatattag caacagtcca gtgccaccat ccactgcctg
     1381 taggagcatc tggatcatac acagttacgg caaagctttt ggtgccttca ggagcaccag
     1441 accaggttaa tgagggcgat gtattaccac cttcacaccc aaatccagaa aatacatgag
     1501 acgttgtaag ttgctctcct gtttttattt cattactagt gacctgaaat gctgcggcct
     1561 gcgcagaaaa tgttatgaat gccaatacac ttgaaacgat aagttttttc ataaaaacct
     1621 cattgttgtg acctatcgtt attttatttg atgtgccttg atctcattat gcataaaggc
     1681 gcaatgatcg cgcaaatgca atcataatta taaaggtgcc ccgaacatta gccataatat
     1741 gcacaaatca gattcctgtt atctcaagga taattatttt ttaatgcggt gcacttcgtg
     1801 acaacaagtt aatgactaac acaccggcac aaatcaacat catgcctatg atagctggca
     1861 ggtccagtcg ttgaccaaaa aatccccagg aaagcaaact aatcagaacg ataccgactc
     1921 ctgaccagat agcataagca atccctgtag gtatataagc cagcgtctga gctaataacc
     1981 agaatgatgc acaatagcaa ataattgtgc caacagaggg ccataaccgt gtaaacccct
     2041 ctgaaaactt cattaaggtt gtacctatga cctctgcaag tattgcgcca ccaagataaa
     2101 tataagggtt catagcatat tctttcctgt tcatactgga gagaattgta ctacagtttg
     2161 aacacaactc accgttttca tcctggtgta tgtgtatcca aggggctctg gcgtggcaag
     2221 agataaaggc aggaacaata atgatcagaa ggaaccccag gctaacgaat gagttcactc
     2281 ttcttgggag caaagcgaag acaaaacatg ttatcgcgtt tacggatcgt ggatggcaga
     2341 aaacaaccag gatcaggtat ccattttaaa ccagaaattg agtgagtttg ccccatccat
     2401 gccccacgct gtgggatcgg atataattaa acaagcatag tcaacaggtt acacaaccta
     2461 cacctgcatg gacatcactt cctgatacgc cgccaccagc ttattacgca cctgaatccc
     2521 catttgcata gaaactgagg ctttttgcat atcggtcatc acatcgttta gcgccacgcc
     2581 gggttcaccg agggtgaatt tttctgcctg cgtgcgggcg actgtttgtg tatcgcttat
     2641 gcgatcgagc gcggcgtgca gctgcccggc aaaactaatg gtcggttgcg gcagtgattc
     2701 ctgcgcacgc gcactcatcg ccgtagcctg taactggctg ataacccctt caatcccctg
     2761 tatcgctgac attttcgtct cccggataat ttctggtaac aaagcctacc agtaagtcaa
     2821 taagacaaag gcgctaaata gcaacaaa
//
LOCUS       DADLSA010000275         2783 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.278, whole genome shotgun sequence.
ACCESSION   DADLSA010000275 DADLSA010000000
VERSION     DADLSA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.278"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            347..1690
                     /gene="argG"
                     /locus_tag="JN691_004203"
     CDS             347..1690
                     /gene="argG"
                     /locus_tag="JN691_004203"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAY7245436.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(1698..>2783)
                     /locus_tag="JN691_004204"
     CDS             complement(1698..>2783)
                     /locus_tag="JN691_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY7245437.1"
                     /translation="FATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
BASE COUNT          737 a          651 c          672 g          723 t
ORIGIN      
        1 ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc tgggatttgg
       61 tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccatgcca cattttgtca
      121 acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt gaatactttt
      181 gccttcccct gctctccctt aagcgcatta ttttacaaaa aacacactaa actcttcctg
      241 tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat tcagtgagag
      301 cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga cgacgattct
      361 caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc tggacaccag
      421 tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata ctgcaaacct
      481 gggccagcca gacgaagagg attatgatgc gatccctcgt cgagccatgg aatacggcgc
      541 ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta ttgccgctat
      601 tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca cgacgccgct
      661 gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat
      721 ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc gttatggtct
      781 gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact ttattgatga
      841 actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg actacaaaat
      901 gtctgtcgaa aaagcctact ccacagactc caacatgctt ggtgcaacgc atgaagcgaa
      961 ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg gcgtgaaatt
     1021 ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg agcaaggtca
     1081 tccggtggcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc tggaagctaa
     1141 ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgagaacc gtatcatcga
     1201 agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca ttgcgtatga
     1261 acgtctgttg accggtattc acaacgaaga caccattgag cagtatcacg cgcatggtcg
     1321 tcagttgggc cgtctgctgt accaggggcg ttggtttgac tcccaggcgc tgatgctgcg
     1381 tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc tggagctgcg
     1441 ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct acaagccaga
     1501 gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta ttggtcaatt
     1561 gaccatgcgt aatctggata tcactgatac ccgagagaaa ctttttggtt atgccaaaac
     1621 tggcctgctt tcctcctctg ccacttcagg cgtgccgcag gtggagaata tggaaaacaa
     1681 aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg cttatcctct
     1741 gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc cacaacccgt
     1801 gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc cggctttgtt
     1861 acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat attgttttcc
     1921 gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat cgggacatga
     1981 tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt cgtagggtca
     2041 cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt taataattca
     2101 aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca ggcatcctga
     2161 tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc cggttcatgg
     2221 cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg taatgcttgc
     2281 gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc catggcgcgc
     2341 atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga gcttagccag
     2401 aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta attatgaatg
     2461 tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt
     2521 gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc aacttgcggt
     2581 gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga ctcccccaca
     2641 atcaacacgt aggtatcaat acctgtatcc ctgactgata attgaaaata cggcaccgtg
     2701 tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata gttgagattg
     2761 aaaaacggcg tataggtagc aaa
//
LOCUS       DADLSA010000276         2768 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.279, whole genome shotgun sequence.
ACCESSION   DADLSA010000276 DADLSA010000000
VERSION     DADLSA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.279"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            37..393
                     /locus_tag="JN691_004205"
     CDS             37..393
                     /locus_tag="JN691_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HAY7245438.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            493..1707
                     /locus_tag="JN691_004206"
     CDS             493..1707
                     /locus_tag="JN691_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7245439.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            1934..>2768
                     /locus_tag="JN691_004207"
     CDS             1934..>2768
                     /locus_tag="JN691_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7245440.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VR"
BASE COUNT          671 a          614 c          606 g          877 t
ORIGIN      
        1 tatattttat tgtttatttc atgcagccat tacacgatga acgcttatga actccaggca
       61 ctccgccata tttttgccat gactattgat gaatgcgcga cctggattgc ccagaccggt
      121 gacagtgaaa gctggcgtca gtgggaaaat ggcaaatgcg ccattcctga ttgtgtagtc
      181 gagcaactgt tggctatgcg tcagcaacga aaaaaacatc ttcatgccat catagagaaa
      241 ataaataacc gcatcggcaa caacaccatg cgttttttcc ccgacttaac cgcgtttcaa
      301 caagtctacc ctgatggcaa ttttattgac tggaagatct atcaatcggt agccgctgaa
      361 ttatatgcac acgatctgga gcggctttgt taatttttcc acagaaagga attgtcgttg
      421 ttacaacaat aatgaatgga tactgacaca acatcgctac actttttaaa gcacctttgc
      481 taagtagaac ctatgaaaaa tccctattac cctaccgcac ttgggttgta ttttaattac
      541 ctggtgcatg gtatgggcgt cattttgatg agcctgaata tggcctcgct ggagacactt
      601 tggcagacta atgccgcggg tgtctcgata gttatctcat cgctgggcat tggtcgatta
      661 agtgtcttgc tttttgcagg attattatcc gatcgctttg gtcgccgccc ttttatcatg
      721 ctcgggatgt gctgctatat ggccttcttt tttggcatcc tgcagaccaa taacatcatt
      781 atcgcttatg tttttggctt tctggcggga atggcaaaca gttttctcga tgcaggcact
      841 tatcccagtt tgatggaagc ttttccacgc tcacctggga cagccaatat tttaattaaa
      901 gcatttgttt ccagcggaca atttttatta ccgctaatca ttagcctgtt agtgtgggct
      961 gaactgtggt tcggttggtc ctttatgatt gctgcaggca ttatgtttat taacgctctg
     1021 tttttatacc gttgtacgtt cccaccccat cctggtcgtc gcttacctgt cataaagaaa
     1081 accaccagct ctacggaaca tcgctgttca attatcgatt tagccagtta taccttatac
     1141 ggctatatct caatggcaac gttttatctg gttagccagt ggctggcaca gtacggacaa
     1201 tttgttgcag gcatgtcata cactatgtcg atcaaactac tcagtatcta caccgtgggt
     1261 tcgctgcttt gtgtatttat taccgctcca ctcattcgta ataccgttcg cccaacaaca
     1321 ttactgatgc tgtacacctt tatctcattt attgctctgt ttaccgtctg cctgcatccc
     1381 acattttatg tggtgataat atttgctttt gtcattggtt ttacctctgc tggaggtgtt
     1441 gtgcaaattg gcctgacgtt aatggctgaa cgtttccctt acgctaaagg taaagctaca
     1501 gggatctatt acagtgcggg cagtattgcg acctttacta ttccgttgat tacggctcat
     1561 ctgtcccaaa gaagtattgc cgatattatg tggttcgata ccgccatcgc tgccatcggt
     1621 tttttactgg cactgtttat cggcttacgc agccgcaaaa aaacgcggca tcactcgcta
     1681 aaggaaaatg tcgctccggg tgggtaatgc aatattcttt tcaggtcatg caagatctta
     1741 cggataaata actctttctg cgctaactaa ggaaaatcgc gatcaaaaac aaactatgac
     1801 atgcaatatt cctggaaaca taaactttat gccatgtacc cagggaaaat catcttcagt
     1861 atagtaatta tgtaaaccgt cggagaacaa tacgtacggt aacgaaatta tctttcagca
     1921 aggagctgtg aaaatgtctc aaaataaggc tttcagcacg ccatttatcc tggctgttct
     1981 ttgtatttac ttcagctatt tcctgcacgg cattagtgtt attacgcttg cccaaaatat
     2041 gacatctctg gcagaaaagt tttccactga caacgcgggc attgcctatt taatttccgg
     2101 tatcggtttg gggcgattga tcagtatttt attcttcggt gtgatctccg ataagtttgg
     2161 tcgtcgggcg gtgatattaa tggcagtaat aatgtatctg ctattcttct ttggtattcc
     2221 cgcttgcccg aatttaactc tcgcctacgg tctggcagtg tgcgtaggta tcgctaactc
     2281 agcgctggat acgggtggct accccgcgct catggaatgc tttccgaaag cctctggttc
     2341 ggcggtcata ctggttaaag cgatggtgtc atttgggcaa atgttctacc caatgctggt
     2401 gagctatatg ttgctcaata atatctggta cggctatggg ctgattattc cgggtattct
     2461 gtttgtactg atcacgctga tgctgttgaa aagcaaattc cccagccagt tggtggacgc
     2521 cagcgtagct aatgaattac cgcaaatgaa cagcaaaccg ttagtctggc tggaaggtgt
     2581 ttcatcggta ctgttcggtg tagccgcatt ctcgaccttt tatgtgattg tggtgtggat
     2641 gcccaaatat gcgatggctt ttgctggtat gtcagaagct gaggcattaa aaaccatctc
     2701 ttattacagt atgggctcgt tggtctgtgt ctttattttt gccgcactac tgaaaaaaat
     2761 ggtccggc
//
LOCUS       DADLSA010000277         2702 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.280, whole genome shotgun sequence.
ACCESSION   DADLSA010000277 DADLSA010000000
VERSION     DADLSA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.280"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..407
                     /locus_tag="JN691_004208"
     CDS             <1..407
                     /locus_tag="JN691_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245441.1"
                     /translation="ADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVED
                     VDNADINQRLLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAAL
                     VYKIFCISESNDARECAAPGAVACRDCGETNA"
     gene            583..762
                     /locus_tag="JN691_004209"
     CDS             583..762
                     /locus_tag="JN691_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003034732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY7245442.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREVEVGHEH"
     gene            752..1693
                     /locus_tag="JN691_004210"
     CDS             752..1693
                     /locus_tag="JN691_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7245443.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRPDASQP
                     DTQTAEQDFLTRHPDAVVFSPKKRQWGTQNDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPEGFRG"
     gene            1788..2441
                     /locus_tag="JN691_004211"
     CDS             1788..2441
                     /locus_tag="JN691_004211"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HAY7245444.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDSDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMVA"
     gene            2438..>2702
                     /locus_tag="JN691_004212"
     CDS             2438..>2702
                     /locus_tag="JN691_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7245445.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISEAGQKFQSAACAAI
                     IEQLRRLPKPTSAHASVEIVLFPPDNRTRDLDNYNKALFDALTH"
BASE COUNT          678 a          652 c          764 g          608 t
ORIGIN      
        1 gtgccgatgg cgatcagatt ttcccgctgg gatgggcaat gattttgcaa cgtgctggtg
       61 gaactcactt cattgctgac gctgtggcgc agtctgcaaa tggcgtcttt gtgtctcttc
      121 ctgacgtcga ggatgtggac aacgccgata ttaaccagcg cctgctggaa gtcattgaac
      181 agatcggcag ttattcaaaa cagattcgtt cagcaattga agacggtgta gtggaaccgc
      241 atgagaagac agcaattaac gacgagctgt acctctcaat ttcgaagctg caggagcatg
      301 cagcactggt ctacaaaatt ttttgcattt cagaaagtaa tgacgcccgc gagtgtgcag
      361 ctccgggcgc cgtggcgtgt cgtgactgtg gagaaactaa cgcatgaaca gtttaacaac
      421 acactaccgt cgctcgcaac tgattgcgct tcctgtaccg ggtggaaaag cgaaggtgga
      481 gtattgctat gcagtaaatg taccaggtga cagggaaatt gtaacccaca gctttgcaga
      541 gtgggctgtg ggtgatttca accggcagaa ggagacagtc ctttgcgaca agttaaccgc
      601 tggttcaaag atcactacgg agtgcccgtc agagtcattc gttgggagcc ggaaacacaa
      661 cgggttatct acctccgcga aggctatgag catgagtgct tcagcccgct cgaacagttt
      721 cgtcgtaaat tcagggaagt agaggtcggt catgagcact aaattaaccg gctatgtatg
      781 ggatggttgc gctgcatcag gcatgaaatt atccagcgtg gcaattatgg cccgcctggc
      841 tgatttcagt aatgacgaag gtgtgtgctg gccatcaatt gaaaccattg cccgtcagat
      901 tggcgcgggg atgagtaccg tcagaacggc tatcgcacgg ctggaagcag aaggctggtt
      961 aacgcgtaag gcgcgtcgcc agggtaaccg caatgcgtcg aatgtttatc agcttaacgt
     1021 tgcgaagctt caggcagcgg cattttctca actgtcagat tctgacccgt caaaatctga
     1081 cgcatcaaaa tctgacccgt caaaatttga tgcgtcgaaa tctggcaaaa aagcgggttt
     1141 tcacccgtca gaatctggcg gggatccgtc agtaaaatca aaacatgatc cgtcagataa
     1201 aaaaccttct cgtccggacg cttcgcaacc ggacacgcag acggctgaac aggatttttt
     1261 aactcgccat cctgatgcgg ttgtattcag ccctaaaaag cgccagtggg ggacgcagaa
     1321 tgatttgacc tgcgcacagt ggctctggaa aaaaatcatc gccctgtacg agcaggctgc
     1381 cgaatgtgac ggcgaggtgg ttcgtcccaa agaaccgaac tggacagcct gggcaaacga
     1441 aattcgcctg atgtgtgtgc aggatggtcg tactcacaaa caaatctgcg agatgtacag
     1501 ccgcgtcagc cgcgatccgt tctggtgccg taacgtgctc agcccgtcga agctgcggga
     1561 aaaatgggat gagctttccc tgcgcttatc gccgtccgtc agcacgtaca cagaaaaacg
     1621 cgaagacccg tacttcaaag ccagttacga caacgtggac tacagccaga tcccggaagg
     1681 attcaggggg tgatcatgag tcttttgaat gaagttcaga aattcattga agcccatccg
     1741 gggtgtactt ccggagacat tgcggatgct ttttacgtgg gggcttaatg agtaataaat
     1801 attgccaggc gctggtagaa ctgcggaaca aaccagccca tgaactgaag gaagtgggcg
     1861 atcagtggcg cacgccggac aacattttct ggggaattaa caccttgttt ggtccgtttg
     1921 ttctggatct gttcactgac agtgataacg ccaaatgtgc cgcgtattac acggcggaag
     1981 acaacgcgct ggcgcatgac tggtcagaac gtcttgcgga gcttaaaggt gctgcctttg
     2041 gcaatccccc gtacagccgc gccagtcagc atgaggggca atacatcacc ggcatgcgtt
     2101 acatcatgaa gcatgccagt gccatgcgtg ataaaggcgg gcgctatgtt ttcctgatca
     2161 aagctgccac cagcgaagtg tggtggccgg aagatgcaga tcatattgct tttattcgcg
     2221 ggcgtattgg ttttgaactg cctgcctggt ttatcccgaa agacgaaaag caggtgccaa
     2281 caggtgcttt cttcgctggt gctattgctg ttttcgacaa gacctggaag ggaccggcaa
     2341 tcagctacat cgggcgcgat gaacttgagg catgtggtga ggcatttctg gcgcaggttc
     2401 gccagcaggc ggaaaaactg gtcagggaga tggtggcatg aagctaatac tgccttttcc
     2461 gcccagcgtg aacacgtact ggcgacaccc caacaaaggg gcatttgctg gtaagagcct
     2521 gataagcgag gcggggcaaa aatttcagag cgcggcgtgt gccgccatca ttgagcagtt
     2581 acgtcgtctg ccgaaaccaa cgtcggcaca tgcttcagtg gagatcgtgt tgtttcctcc
     2641 ggataacagg acccgcgatc tggacaacta taacaaggcg ctgtttgacg ccctgaccca
     2701 cg
//
LOCUS       DADLSA010000278         2697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.281, whole genome shotgun sequence.
ACCESSION   DADLSA010000278 DADLSA010000000
VERSION     DADLSA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.281"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            258..1673
                     /gene="gltX"
                     /locus_tag="JN691_004213"
     CDS             258..1673
                     /gene="gltX"
                     /locus_tag="JN691_004213"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAY7245446.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(1725..2096)
                     /locus_tag="JN691_004214"
     CDS             complement(1725..2096)
                     /locus_tag="JN691_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY7245447.1"
                     /translation="MTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGGRARLILV
                     DTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTDIEQEKVA
                     QFLSREGIRNFLARLDIDESA"
     gene            complement(2119..2463)
                     /locus_tag="JN691_004215"
     CDS             complement(2119..2463)
                     /locus_tag="JN691_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY7245448.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
BASE COUNT          668 a          648 c          672 g          709 t
ORIGIN      
        1 cgctgtgtgg agtcgcatta tagggagagt tcaaaatgag tcaacgcatt ttctaaagaa
       61 attgttcgtt cgtcgtaaat ttaagcaaga tgatcgcaaa acagaccgtg ttgcgcaatt
      121 tgtcaacgaa aacaataatg cgtaaggtag aaacccgaac tacattgagg aatcaggcgg
      181 gagtgataga atatcgccca ctcaattttt ccaggatttg ccggttgtcg gcatctttct
      241 aaacgtaagg ccatttcatg aaaatcaaaa ctcgcttcgc gccaagccca acaggctatc
      301 tgcacgttgg cggcgcgcgt actgctcttt actcctggct ttttgcacgt aaccacggcg
      361 gtgagttcgt gctgcgtatt gaagacaccg atcttgagcg ttccacgccg gaagctatcg
      421 aagccattat ggatggcatg aactggctga gcctggagtg ggatgaaggt ccgtactacc
      481 agaccaaacg ttttgatcgc tacaacgcgg tgatcgatca gatgctggaa gagggcactg
      541 cttataaatg ctattgctct aaagagcgcc tggaagcgct gcgcgaagag caaatggcga
      601 aaggtgagaa gccgcgttat gacggtcgct gccgccacag ccatgagcat catgctgatg
      661 atgaaccgtg tgttgtacgt tttgctaacc cgcaggaagg ttctgttgtt tttgacgatc
      721 agatccgtgg tccgatcgag ttcagcaacc aggaactgga cgatcttatt atccgccgta
      781 ccgatggttc cccaacctat aacttctgtg tggttgtcga tgactgggat atggaaatca
      841 cccacgttat ccgtggcgaa gaccatatca acaacacgcc gcgccagatc aacattctta
      901 aggccctgaa agcgccggtg ccggtttacg cgcacgtttc tatgatcaat ggcgatgacg
      961 gtaaaaaact gtccaaacgt cacggggcag tcagcgtaat gcagtatcgt gatgacggtt
     1021 atttgccaga agcactgctg aactatctgg tgcgtctggg ctggtcccat ggcgatcagg
     1081 aaatcttcac tcgtgaagag atgatcaaat acttcacttt gaatgccgtc agcaaatctg
     1141 ccagtgcgtt caacaccgac aagctgctgt ggctgaacca tcactacatt aacgcgctgc
     1201 cgccggagta tgttgctact cacttacaat ggcacattga gcaggaaaat atcgataccc
     1261 gtaacggccc gcagctggct gatctggtga aactgctggg cgaacgctgc aagacgctga
     1321 aagagatggc acagagctgc cgttatttct acgaagattt tgctgagttc gatgccgatg
     1381 cggcgaaaaa acatctgcgt ccggtagcgc gtcagccgct ggaagtggtt cgtgacaaac
     1441 tggccgcgat tactgactgg accgctgaaa acgttcatca cgctattcag gcgacggcgg
     1501 atgagctgga agtgggtatg ggtaaagttg gtatgccgct gcgtgtcgcc gtaaccggtg
     1561 cggggcagtc tccagcactg gatgttaccg ttcacgcaat tggtaagacc cgcagtatcg
     1621 agcgtatcaa caaagcgctg gattttattg ctgaacgcga aaatcagcag taattagcgt
     1681 gtaaaaagat aaacggcagg agataatatc ctgccgtttt ttatttatgc tgattcgtca
     1741 atatcgagac gggcgaggaa attgcgaatt ccttcacgtg acaaaaattg cgcgactttt
     1801 tcctgctcaa tgtctgtcat attatctatc gcgttgatga tttgtcgata cgccggggcg
     1861 cgtgctccag gagcgtattc agcaattctc tcttcagctt ccatcaacat tggtgtgtta
     1921 tggaaggctg gcgtgttctg aataaactcg caaacttgcg tatcgaccag aataagtctg
     1981 gcacgaccgc ctttcacgcc gggaaatttt tccgttttcc agcctttttc tctgatccaa
     2041 cggttaacag tttgtttagc cacaccgagg cattcagcca gttcttcggt ggtcatttta
     2101 ttgcgtaatc ttttcatatt atttgctatc gcgaatccct aagcgttgta acaatccgat
     2161 aatcccttcc cgcagaagca aggatgtaaa ctgtttttgt tcaactggcg tcatctcttt
     2221 cgccagtgtc accagcaata cttcaagcgg cgcatcacca gaaagggcag gcgcttctcc
     2281 ctgaccttct ggacgttcag cattgcgaat atattcacga acctgttcat tgacgtgaac
     2341 cagtcgggct ttgccaccct ggacgccagg ttttggtgac gttgtccagc cttccttgcg
     2401 tacccattta ttaatggtct ggcggctata gccggtcagt ctggcaagtt catctggtgt
     2461 catcctctcc ttgaacataa caattccctg aatgtatgtg ggtctattag tagttcattt
     2521 tataacacca ctttacagga aaccgtgaca tgaataacta ctgaatgcga gcggttttcc
     2581 aaaattgttc atttgcctga tttttcagcg attgaaacta cacgttgcaa tttgccgttg
     2641 acacattcgg gcggaattca tatgatgccg cccgtcaact cgacaagctt tacgtga
//
LOCUS       DADLSA010000279         2640 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.282, whole genome shotgun sequence.
ACCESSION   DADLSA010000279 DADLSA010000000
VERSION     DADLSA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.282"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(1..69)
                     /locus_tag="JN691_004216"
                     /pseudo
     CDS             complement(1..69)
                     /locus_tag="JN691_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            280..369
                     /locus_tag="JN691_004217"
                     /pseudo
     CDS             280..369
                     /locus_tag="JN691_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            393..>2640
                     /locus_tag="JN691_004218"
     CDS             393..>2640
                     /locus_tag="JN691_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAY7245449.1"
                     /translation="MGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFRDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSREGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNV"
BASE COUNT          633 a          667 c          689 g          651 t
ORIGIN      
        1 aagccagtag tctgcccacc actgcatcat ttctctgcgc ttatcgagat actgagcatg
       61 gttgtaaatt cagctaaccc agtgcgttga caaaaaatta gcgcaagaag acaaaaaatc
      121 accttgcgct aatgctctgt atcaggtcac taataccagc taagtagttg attcatagtg
      181 actgcatatg ttgtgctttg tcgcattttg tagtctatta tttaatctgt aacaacgata
      241 atacaatgaa ttttaattgg tttttgtgat tttgtccttc ttctgacatg gaaggacacc
      301 cgatggatac ccaacgattc caatcccaat ttcattggca tttatcgttt aaattttctg
      361 gcgcgatagc agcgtgcttg tccctgtctc ttgtgggaac agggcttgct aaagctgatg
      421 attcgcttcc ttccagtaac tatgcgccgc ccgccggggg aacattcttt ttgcttgctg
      481 acagcagttt tagcagcagt gaagaggcga aagtgcgact ggaagcgccg gggcgtgatt
      541 atcggcgcta tcagatggaa gagtacggcg gcgtggacgt tcgcctgtat cgtattcctg
      601 acccgatggc atttttgcgc cagcagaaaa acctgcatcg cattgtggtg caaccgcaat
      661 atctgggcga cgggctgaac aatacgctga cctggctgtg ggataactgg tacggcaaat
      721 ctcgccgcgt gatgcagcgt actttctctt ctcagtcacg gcagaatgtg actcaggcat
      781 tacccgaatt acatctcggc aatgccatta ttaaaccttc ccgttatgta cagaacaacc
      841 agttttcccc gctgaaaaaa tatcccttgg tggaacagtt ccgttatcca ctatggcagg
      901 ctaaaccggt cgagccgcag caaggggtaa aactggaagg cgcatccagc aatttcatct
      961 cgccgcagcc gggtaatatt tatattcctc tcggccaaca agagccgggg ctgtacctcg
     1021 tcgaggcgat ggtaggtggg tatcgggcga cgacggtggt gtttgtttcc gataccgtgg
     1081 cgcttagcaa agtgtcaggt aacgagcttc tggtatggac cgcgggtaaa aaacagggtg
     1141 aagcgaagcc cggctcagag atcttgtgga ctgacggtct tggcgtgatg acccgcgggg
     1201 tgaccgatga cagcggtact ttgcagttac aacatatatc gccagaacgt tcatacattc
     1261 tgggtaagga tgctgaaggc ggcgtttttg tctccgagaa cttcttctac gaaagcgaaa
     1321 tctacaacac ccgcttgtat atttttaccg atcgcccgct atatcgcgca ggcgatcgtg
     1381 tcgatgttaa agtgatgggc cgcgagttcc gcgatccgtt gcattcatcc cccatcgtca
     1441 gcgccccggc gaagctttcg gtgctggacg ctaacggcag tctgttgcaa accgtcgatg
     1501 tcacgctgga tgcgcgcaat ggcgggcagg gaagtttccg cctgccagaa aatgccgttg
     1561 ccggaggtta tgagttacgt cttgcttacc gcaatcaggt ctatagcagc agttttcgcg
     1621 tggcaaacta catcaagcca catttcgaga ttggtttagc tctcgacaaa aaagagttca
     1681 aaactggcga agcggtcagc ggcaaactgc aactgctcta cccggatggc gagccggtaa
     1741 aaaatgcccg cgtgcagtta agtttgcgcg ctcagcaatt atcaatggtc ggtaacgatt
     1801 tgcgttatgc cggacgtttc cccgtgtcgc gggaaggcag cgaaacggtg tccgacgcca
     1861 gcggtcatgt ggcgttaaat ctccccgccg ccgataaacc gagccgctat ttgttaaccg
     1921 tctccgccag tgacggcgcg gcgtatcgcg tcaccaccac caaagagatc ctcattgaac
     1981 gcggcctggc gcattactct ttaagtaccg ccgcacaata cagtaatagc ggcgagtcgg
     2041 ttgtgttccg ttatgccgcg ctggaatctt caaaacaggt tcctgttacg tatgaatggt
     2101 tgcgtctcga agaccgcacg agccatagcg gagagctacc gtcaggcggc aaatccttta
     2161 ccgtcaattt cgctaaacct ggcaactaca atctgacatt acgcgataaa gacggcttaa
     2221 ttctcgctgg gttaagtcat gccgtcagcg gtaagggcag cacggcgcat actggtacgg
     2281 tagatatcgt ggcggataaa acgctgtacc agccaggcga aaccgcgaag atgctgatta
     2341 ctttcccgga gccaattgat gaagcgttat tgacgctgga acgcgatcgc gtggaacagc
     2401 agtcgctgct ttcgcatccg gcaaactggc taacgctaca acgtttaaac gatacccagt
     2461 atgaagcccg ggttccagtg agcaattcct ttgcgcctaa catcactttt tcggtgctgt
     2521 atacccgtaa tggtcagtac agttttcaga acgccgggat caaagttgcc gttcctcagc
     2581 ttgatatccg ggtgaaaacg gacaaaaccc attaccagcc tggtgaactg gtcaatgtcg
//
LOCUS       DADLSA010000280         2599 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.283, whole genome shotgun sequence.
ACCESSION   DADLSA010000280 DADLSA010000000
VERSION     DADLSA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.283"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            176..355
                     /locus_tag="JN691_004219"
     CDS             176..355
                     /locus_tag="JN691_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245450.1"
                     /translation="MTLVSVLATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDF
                     MMACDMYFRWGHFNK"
     gene            complement(548..635)
                     /locus_tag="JN691_004220"
     tRNA            complement(548..635)
                     /locus_tag="JN691_004220"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(599..601),aa:Ser,seq:gga)
     gene            869..1807
                     /gene="ghrA"
                     /locus_tag="JN691_004221"
     CDS             869..1807
                     /gene="ghrA"
                     /locus_tag="JN691_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HAY7245451.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGCKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            1862..2599
                     /locus_tag="JN691_004222"
     CDS             1862..2599
                     /locus_tag="JN691_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAY7245452.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHLVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
BASE COUNT          629 a          593 c          662 g          715 t
ORIGIN      
        1 gctgcggcag taacaccgat gcgttcagca agcttcaggg cttcactgcg aaattcaggc
       61 gaatgctgtt tacggggttt tttactggtt gatactgttt ttgtcatgtg agtcacctct
      121 gactgagagt ttactcactt agccgcgtgt ccactattgc tgggtaagat catggattac
      181 gctggtgtcg gtattggcaa cgcttgtgga atggcggatt tggtttgaag cctatttttt
      241 actctgctat gtaccgtgct taatttacta ttttttccat tgtcgattcc tctggcataa
      301 cgattttatg atggcatgtg acatgtattt ccgttggggg cattttaata agtgaggaag
      361 ttacaggaag tgaccagata acgcatatat gttctgtact atcttgcgca ttttgattgt
      421 tgactgagta accagacagt tgatgtgcac aatttcccct cgccctaaca gacgtgggcg
      481 ggggcacccg gtgtgtacaa tatacgaaaa aaaagcccgt actttcgtac gcgctcttct
      541 ttaaatatgg cggtgagggg gggattcgaa cccccgatac gttgccgtat acacactttc
      601 caggcgtgct ccttcagcca ctcggacacc tcaccaaatt gtcgttcctg tcttgctgga
      661 acgggcgcta atttagggaa atcatgacct gaggtcaaca aactttttga aaaaatcgcg
      721 cgtttattca aacttcaatc aatgtgtggt tttaataagc gaaatctgct ttttttgcca
      781 ccgaccacgg atttgttatg ctggtggcct ttgtagatca taacgataag tgcgaataaa
      841 tttcgcacaa cgcttttcgg gagtcagtat ggatatcatc ttttatcacc caacgttcga
      901 tacccaatgg tggattgagg cactgcgcaa agctattcct caggcaagag tcagggcatg
      961 gaaaagcgga gataatgact ctgctgatta tgctttagtc tggcatcctc ctgttgaaat
     1021 gctggcaggg cgcgatctta aagcggtgtt cgcactcggg gccggtgttg attctatttt
     1081 gagcaagcta caggcacacc ctgaaatgct gaacccttct gttccacttt ttcgcctgga
     1141 agataccggc atgggcgagc aaatgcagga atatgctgtc agtcaggtgc tgcattggtt
     1201 tcgacgtttt gacgattatc gcatccagca aaatagttcg cattggcaac cgctgcctga
     1261 atatcatcgg gaagatttta ccatcggcat tttgggcgca ggcgtactgg gctgtaaagt
     1321 tgctcagagt ctgcaaacct ggcgctttcc gctgcgttgc tggagtcgaa cccgtaaatc
     1381 gtggcctggc gtgcaaagct ttgccggacg ggaagaactg tctgcatttc tgagccaatg
     1441 tcgggtattg attaatttgt taccgaatac ccctgaaacc gtcggcatta ttaatcaaca
     1501 attactcgaa aaattacctg atggcgcgta tctcctcaac ctggcgcgtg gtgttcatgt
     1561 tgtggaagat gacctgctcg cggcgctgga tagcgggaaa gttaaaggcg caatgctgga
     1621 tgtttttaat cgtgaaccct taccgcctga aagtccgctc tggcaacatc cacgcgtgac
     1681 gataacacca catgtcgccg cgattacccg tcccgctgaa gctgtggagt acatttctcg
     1741 cactattgcc cagctcgaaa aaggggagag ggtctgcggg caagtcgacc gcgcacgcgg
     1801 ctactaataa agcatcagga ttcctgctat ccttggcggg aattgaatac aggagagagt
     1861 tatgtatccc gtcgaccttc atatgcatac cgttgccagc acacatgcat atagcacatt
     1921 gagtgattac attgcccagg ccaaacaaaa gggcattaaa ctttttgcga tcaccgatca
     1981 tggcccggat atggaagatg cgccgcatca ttggcacttc attaacatgc gtatctggcc
     2041 gcgagtggtt gatggggtag ggatcctgcg cggcattgaa gctaacatta aaaatgttga
     2101 tggtgaaatt gactgcagcg gtaaaatgtt tgactcgctg gatctgatta ttgccggttt
     2161 tcatgagccg gtttttgcgc cacatgacaa agcgaccaat acacaagcga tgatcgccac
     2221 tatcgccagc ggcaatgtgc atataataag ccatcccgga aatccgaaat atgaaataga
     2281 tgttaaagcc gttgctgaag cagccgcgaa acatctggtg gcgctggaaa tcaataattc
     2341 ctcattttta cactcacgta agggcagtga agacaactgt cgtgcggtag ctgcagcggt
     2401 acgtgatgct ggaggttggg tggcattagg ctcggattct cacaccgcgt ttaccatggg
     2461 ggaatttgaa gagtgtctta aaatcctcga cgcggtagat tttccgccag agcgcatttt
     2521 gaatgtttct ccgcgccgct tactgaactt ccttgaatct cgcggtatgg caccgattgc
     2581 ggaatttgca gacctttaa
//
LOCUS       DADLSA010000281         2539 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.285, whole genome shotgun sequence.
ACCESSION   DADLSA010000281 DADLSA010000000
VERSION     DADLSA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.285"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            368..2020
                     /locus_tag="JN691_004223"
     CDS             368..2020
                     /locus_tag="JN691_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY7245453.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPKITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIREHSPLDAASYEPKGEVSWQ"
     gene            complement(2031..2201)
                     /locus_tag="JN691_004224"
                     /pseudo
     CDS             complement(2031..2201)
                     /locus_tag="JN691_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2280..2393)
                     /locus_tag="JN691_004225"
                     /pseudo
     CDS             complement(2280..2393)
                     /locus_tag="JN691_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989794.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          763 a          504 c          593 g          679 t
ORIGIN      
        1 atcggaaacg atgatctggc agtctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtccgtgaag ggaataaggc
      241 tcaggtcagc aaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggtacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg gaaaacggaa ttttcttttt acacccccaa
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg gaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg gatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccgtat tgatatttac
     2281 taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggtcagtcc
     2341 tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt cagacggcat
     2401 gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctattgga accccgccgg
     2461 agagagtttc actctccggc tgctcacgga accggacgtg aacctctcag ctcatccggc
     2521 tcttctcatt gaggcctgt
//
LOCUS       DADLSA010000282         2505 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.286, whole genome shotgun sequence.
ACCESSION   DADLSA010000282 DADLSA010000000
VERSION     DADLSA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.286"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            135..564
                     /locus_tag="JN691_004226"
                     /pseudo
     CDS             135..564
                     /locus_tag="JN691_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            751..915
                     /locus_tag="JN691_004227"
                     /pseudo
     CDS             751..915
                     /locus_tag="JN691_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1409..1987
                     /locus_tag="JN691_004228"
     CDS             1409..1987
                     /locus_tag="JN691_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245454.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(2096..2354)
                     /locus_tag="JN691_004229"
                     /pseudo
     CDS             complement(2096..2354)
                     /locus_tag="JN691_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2338..2505
                     /locus_tag="JN691_004230"
                     /pseudo
     CDS             2338..2505
                     /locus_tag="JN691_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          756 a          457 c          642 g          650 t
ORIGIN      
        1 attcctgccg gtatcagcgc cgacggcatg tattgccctg aatgcggtac ggttcactgg
       61 ccggatggcg ttattccgcc tttctgatgc ttccccgttt tgccgatatt tttcagcagg
      121 gaaaccgatg gcttaactgg ctggagaagc agccggtgca gatgagccgc ctggaacatt
      181 atgccggtca ggatgaaatt ggcctgcgtt acaacagcca ccggacaaaa cgagaagaaa
      241 accttgtgat gagtggcgac gagtttatgg aaaggttctc ctggcatgtg gcggataagg
      301 ggttccggat ggtgatacgg ggtcctgagt ccggtgaagc ggcgattact ggaagatgtg
      361 gtgtacgcca taacggagac agtgagaaaa acggcgaggc aaatcacaag gagagggatg
      421 tatcagcagt gactgaaggt tgacccgctg aagtgcgagc tgtgcggagg tcagatgcgg
      481 tttacggggc tgaagagggg ttaccgtctg gcagagcagg ttatgatgca tgagccgctg
      541 gctcgaatac ggtggtgcgg ctgagagtcg cagaggggaa gttgcgtcca tgttgagggg
      601 aacggagcaa aaaaccatca gagatacatt gaatcaatca atgtaagcaa tattaagtgt
      661 ctataccgat tacttgatga tcaggttgtt ttgaataaac gcgcattctt cggtgctttt
      721 ttaatctttt ggggatttaa attcctatcg atgaactgtc gatatgaaaa agcgagcatc
      781 atactgacgt caaacaaagg ggtcgcagac tggggagaaa tgttcggaga tcacgtgctg
      841 gcaacagcga tcctagaccg gctgctacac gctgaatatc aaaggagaga gttaccggtt
      901 aaaagagaaa cgtaaagctg gagtgctgac caaaaacaca acgccaatca gtgatgatga
      961 aatggtggaa aacggacagc atcagtaacg aaagtatctt agcaggcatg aaaatcgtcc
     1021 gcatccccta aacggtcaaa atgcttctgc cgtgaaggag ggagaatgcc atctcatcac
     1081 ttatatctgg cgaatgaaac acagatggtc ggccttgccc ccaaacacac ggcggtgcaa
     1141 aatggaatgg cagaaaactt cgtcaaaacg atgaaatatg attaaatcag tgttatgttc
     1201 gaacctgacg tgttaacagc agtaaagtag tctcgcgaag gtgttcaaac attataatga
     1261 atggcatcct catagtgcgc agggttatcg cttgctgcgg aaatatctga agcgacgtac
     1321 cagtaatggg gaaaatgata gctggtttct gaaaatatag aggaaaatgt gatatatata
     1381 tcctgttaat atttaaggag aatataaaat gaaagtctcg tttaagtctt taggctatat
     1441 ttttcatgat atatataata aaaaacacac tatcgatgag ttcaacgatg tggtaagaaa
     1501 agcagtttta tcaggtaaaa ttaacgagct gaatgcatgt cataaagtgg caatttttct
     1561 tgccgaaaaa gacaatgaga taacaaagaa agacaaagcc aagataatag atacattaac
     1621 tgaaaactat tccattgaat ttcaacaatt aatgaatatt agtgagcgca cgttaaattc
     1681 atctttatat attactcctg gtgaaagtgg ttttgtcagt tttgttaatc gtgaaggaaa
     1741 aatatgtcat actgcatatg taaaatcatc agataatagc atggcatatt accatgcaaa
     1801 ttatagcagc attgacaaat atattacgga tatgtgcggc cttatttgta tgaggcatat
     1861 tgagtctact ggcattattt tttatatgct tgatgaaaaa gttttatctg ccattgctga
     1921 atttatgaat gaaaaaggat ggcgggctgc gttttgttca gcaaagaact tatataaatg
     1981 tgtttaattt tagaaaaact atttggtaaa atccaacaag agtcgagagg ttcgcgttgc
     2041 ttcagcaggg cctcagcctg ttcaaccaga ctcagttgca ggtcgtgacg cagatttagt
     2101 ggtggtggtt gattgtcaga taccagccga tgatcttgta gtgcatatca tcagactttg
     2161 agaagcatat ggttttgcgg gtaagccgct taagatgtgt ccgcaggttc agattatgtc
     2221 gctctatccg ctgagtgcat ttcttgctta taatatgact ggttgaggac aataaagttc
     2281 ggtataccgg ctcaggcatc ggtcatgtag aaggcaatgc taaactggct cagcaaacta
     2341 agagcagcgc gcatccactt acccaatgcg gttgagaaaa tagccttcga tcgctttcat
     2401 gtggcgaaac aaccgggtga ggtggtggat aaaacccgcc agaatgaaca tccgcatctt
     2461 cctgttgaaa gtcgtcgtca ggcaaaggga actcgcttcc tgtgg
//
LOCUS       DADLSA010000283         2476 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.287, whole genome shotgun sequence.
ACCESSION   DADLSA010000283 DADLSA010000000
VERSION     DADLSA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.287"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(283..1143)
                     /locus_tag="JN691_004231"
     CDS             complement(283..1143)
                     /locus_tag="JN691_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAY7245455.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1326..1883)
                     /locus_tag="JN691_004232"
     CDS             complement(1326..1883)
                     /locus_tag="JN691_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7245456.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            2047..>2476
                     /locus_tag="JN691_004233"
     CDS             2047..>2476
                     /locus_tag="JN691_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HAY7245457.1"
                     /translation="MPVDFLTTEQELNYGRYVAEPNDVQLARYFHLDERDLAFINQRR
                     GRHNRLGIALQLTTARFLGTFLTDLTRVLPGVQQFVAVQLNIRRPEVLTRYAERVTTL
                     REHTALIKEYYGYHEFGDFPWSFRLKRLLYTRAWLSNERPG"
BASE COUNT          616 a          631 c          562 g          667 t
ORIGIN      
        1 gctcgggtcg gtggctctgg taacgaccag tatcccgatc ccggctggcc gtcctggccg
       61 ccacatgagg catgttccgc gtccttgcaa tactgtgttt acatacagtc tatcgcttag
      121 cggaaagttc ttttaggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
      181 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
      241 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      301 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      361 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      421 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
      481 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
      541 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc
      601 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
      661 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
      721 tccgatcgtt gtcagaagta agttggcagc agtgttatca ctcatggtta tggcagcact
      781 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
      841 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
      901 acgggataat accgcaccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
      961 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     1021 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     1081 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacgaaaat gttgaatact
     1141 catactcttc ctttttcaat attattgaag catttaccag ggttattgtc tcatgagcgg
     1201 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1261 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     1321 gcgtatcacg aggccctttc gtcttcaaga attttataaa ccgtggagcg ggcaatactg
     1381 agctgatgag caatttccgt tgcaccagtg cccttctgat gaagcgtcag cacgacgttc
     1441 ctgtccacgg tacgcctgcg gccaaatttg attcctttca gctttgcttc ctgtcggccc
     1501 tcattcgtgc gttctaggat cctccggcgt tcagcctgtg ccacagccga caggatggtg
     1561 accaccattt gccccatatc accgtcggta ctgatcccgt catcaatgaa ccggactgcc
     1621 acgccctgag cgtcaaattc ctttatcagt tggatcatat cggcagtgtc gcggccaaga
     1681 cggtcgagct tcttaaccag aatgacatca ccttcctcca ccttcatcct cagcaaatcc
     1741 agcccttccc ggtctgttga actgccggat gccttatcgg taaatatacg gtttgctttc
     1801 acacctgcgt ctttgagtgc tctgacctga agatcaagag actgctgact ggttgagacc
     1861 cgagcgtaac caaaaagtcg cataaaaatg taccttaaat cgaatatcgg acaactcatg
     1921 tctattatta caaatttacg atttaataga catattaatg taacagtttt acgatgtccg
     1981 ataatttata atatttcgga cggttgcgaa attgttaatt catgaccatc aggcagggag
     2041 gccggtatgc ccgtcgattt tctgaccact gagcaggaac tgaattatgg tcgctatgtt
     2101 gcagaaccca atgacgtgca gctggcgcgc tattttcatc ttgatgagcg ggatcttgcc
     2161 ttcattaacc agcggcgggg aaggcataac cggctgggaa ttgcgcttca gctcaccacc
     2221 gcccgttttc tgggcacctt tctgacagac ttaacccggg ttctgccagg tgttcagcaa
     2281 tttgtcgcgg tgcagcttaa tattcgccgt ccggaagtcc ttacccgcta cgcagaacgg
     2341 gttaccaccc tcagagagca tactgcgctg attaaggaat attacggtta ccatgaattt
     2401 ggtgatttcc cctggtcttt ccgcctgaag cgcctgctgt acacccgcgc atggctcagt
     2461 aatgagcgac ccggcc
//
LOCUS       DADLSA010000284         2439 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.289, whole genome shotgun sequence.
ACCESSION   DADLSA010000284 DADLSA010000000
VERSION     DADLSA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.289"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..565
                     /locus_tag="JN691_004234"
     CDS             <1..565
                     /locus_tag="JN691_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7245458.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="JN691_004235"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="JN691_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HAY7245459.1"
                     /translation="MEHSRIKKRNVALIEKCIMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="JN691_004236"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="JN691_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAY7245460.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..2403
                     /locus_tag="JN691_004237"
     CDS             2167..2403
                     /locus_tag="JN691_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAY7245461.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          628 a          536 c          625 g          650 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcatcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgc ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gcagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccggtttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctagcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta
     2341 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
     2401 tgacaaaaca gggaagaaac aagaaaggaa gtaaagata
//
LOCUS       DADLSA010000285         2435 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.290, whole genome shotgun sequence.
ACCESSION   DADLSA010000285 DADLSA010000000
VERSION     DADLSA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.290"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            1..310
                     /locus_tag="JN691_004238"
                     /pseudo
     CDS             1..310
                     /locus_tag="JN691_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108985.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            336..644
                     /locus_tag="JN691_004239"
     CDS             336..644
                     /locus_tag="JN691_004239"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245462.1"
                     /translation="MNVEKEIYTGLYLFGNMKLNTSCGGVSAVIKVPTYNSNVLFFIN
                     DSHVSEEDSMENAKCYKFNLEELESIQKAIERALNDIRNLESLSRRNLLKDKAYRNPI
                     "
     gene            815..895
                     /locus_tag="JN691_004240"
     CDS             815..895
                     /locus_tag="JN691_004240"
                     /inference="COORDINATES: protein motif:HMM:NF015490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245463.1"
                     /translation="MWYYYTDLTGTVQEVTASDGTLVWAG"
     gene            883..1779
                     /locus_tag="JN691_004241"
     CDS             883..1779
                     /locus_tag="JN691_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAY7245464.1"
                     /translation="MGWLTSRTGQMYDHDHYYYDKAGNLLTDEYQSAVMDNRLPGYGR
                     DRYRYNEWGELTERRDQQLEWNAQGQLTRVISSNSDTRYQYDALGRCISKATYGRHTD
                     RGERSRTTFVWEGFRLLQETTWQGKRTYLYDAEQPYTPVAAITGRGESQKIWYYHTDL
                     TGTVHEVTAPDGTLVWAGYQAGFGENRGDISNSGAYFEQPQRLPGQYFDEETGLHYNL
                     FRYYAPECGRFVSQDPIGLNGGLNLYAYAPNPLGWIDPLGLNSLGIDSNNIFRGDGNY
                     KGGGIGQPKSGTISAQDIIDHL"
     gene            1879..2082
                     /locus_tag="JN691_004242"
     CDS             1879..2082
                     /locus_tag="JN691_004242"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245465.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            2100..2420
                     /locus_tag="JN691_004243"
     CDS             2100..2420
                     /locus_tag="JN691_004243"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245466.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
BASE COUNT          766 a          436 c          659 g          574 t
ORIGIN      
        1 ttatggttta ctcaaaacac aatatcagac gtgtttacac aaggaaaatg ggccgggaga
       61 tcggttgaag aagcgattca tgaggctagt gtcacggggc ggcttcctga tgggctgagt
      121 attagttatg aaaagataat tctgccaagc ggggaggagg tttgggcgac tctaaataat
      181 cgtacgcttt atgtggccca acaggcaaat gttacaatta acccagtaga taaaaatgga
      241 aaaggattaa atcagttgaa taaacttttg gatggaaggg cacccactcc agaacaaccg
      301 gatgttttaa taaaacactg ctaacaggat gcgttatgaa tgtagaaaaa gaaatttata
      361 cagggcttta tttgtttgga aatatgaaat tgaacacatc atgcggaggt gtatctgcag
      421 ttatcaaagt accgacatat aatagtaatg ttttattttt tataaatgat tcacatgtaa
      481 gtgaagaaga tagcatggaa aatgcgaaat gttataaatt taacttagaa gaattagaaa
      541 gtatacaaaa ggcgattgaa agagctttaa atgacatacg aaatctagag tctttatcac
      601 gcaggaattt gcttaaggat aaagcttata gaaaccccat ataatccagc actctgaagt
      661 aatgcgggcg cacaacttgc cctcatatag acgcagggaa tacaagctgt tgctggaaat
      721 accatagcag agcgagcgca ggatgtatct ttacgactcg gagcaggcgt acaccccggt
      781 ggcgagcgtg acggggcgct gagaaagccg aaaaatctgg tattactaca cagacctgac
      841 aggcacggtg caggaggtga ccgcgtcgga cgggacgctg gtgtgggctg gctgaccagc
      901 cgcacgggtc agatgtacga ccatgaccat tattattacg acaaagcggg caacctgctg
      961 acggacgagt atcagagcgc ggtgatggat aaccgcctgc cgggctacgg gcgtgaccgt
     1021 taccgctata acgaatgggg cgaactgaca gagcgcaggg accagcaact ggagtggaac
     1081 gcgcagggcc agttaacgcg ggtcatcagc agcaacagcg atacgcggta tcagtatgat
     1141 gcgctgggca ggtgcatcag caaggcgaca tatggccgcc atacggaccg gggagaacga
     1201 agccggacga cgtttgtgtg ggaagggttc cggctgttgc aggagacaac gtggcaggga
     1261 aagcggacat acctgtacga cgcggaacag ccgtacacgc cggtggccgc catcacgggg
     1321 cgcggagaga gccagaaaat ctggtattac cacacggacc tgacgggcac ggtacacgag
     1381 gtaaccgcgc cggacgggac gctggtgtgg gcgggctacc aggcgggatt tggtgaaaac
     1441 cggggcgaca tcagcaacag cggggcgtac tttgagcagc cgcagcggct gccggggcag
     1501 tactttgacg aagaaacagg gctgcattac aatctgttca ggtattatgc gccggagtgc
     1561 gggaggttcg taagccagga cccgatagga ttaaacggtg ggttgaactt atatgcttat
     1621 gcgcctaatc cgttaggttg gatcgatccg ctaggactga attctttagg aatagattca
     1681 aataatatat ttagaggaga tggtaactat aaaggtggcg gaattggaca gccaaagagt
     1741 ggcacaatat cagcacagga cattattgat catctctaag aaaaaggtga tgggcgttta
     1801 acatctttta gtactaaaaa aacatctagt tccaactctc gtggtgcaga ttttttctca
     1861 aaaaaccatg tcttcaaagt ggcagtttct gatctcgatg atttagttaa aaataatcag
     1921 atcactgttt taacacaaga aaaagtaaaa gcaatatttg gtttagaatc aagcaagaaa
     1981 attagaaaag aacttggtaa tattcttcag aatatggcta ggaataatga ggtgttagtt
     2041 catgggcaaa ttcttggaaa taaagttagc cgatgtaatt agttagtgga gaggaagttg
     2101 tggacgaaat tagaataaga attgctggta atacggctgc gccttgtttc catgtgctaa
     2161 aaaataaggg atataaagta acaatggaaa ctcatgcatt atctgacgat tgtttagatt
     2221 gtgtatatga ttggtatgcg gaaaaaaatg gacaattttt tagtgcaacg agtccagaag
     2281 agttacttgg attagttgca atgtgggaag ttcgtggtaa tgactggaga gctaatttga
     2341 atgagataaa tacttatcaa aaagccatag aagaatcccc gttttttgat aaaaacggca
     2401 atgaaataga gtctgactaa attttattga tatag
//
LOCUS       DADLSA010000286         2422 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.291, whole genome shotgun sequence.
ACCESSION   DADLSA010000286 DADLSA010000000
VERSION     DADLSA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.291"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..420
                     /locus_tag="JN691_004244"
     CDS             <1..420
                     /locus_tag="JN691_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7245467.1"
                     /translation="LTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLA
                     ITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEI
                     GFSSGFYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            463..966
                     /gene="yeaK"
                     /locus_tag="JN691_004245"
     CDS             463..966
                     /gene="yeaK"
                     /locus_tag="JN691_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAY7245468.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(967..1071)
                     /locus_tag="JN691_004246"
     CDS             complement(967..1071)
                     /locus_tag="JN691_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245469.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            1241..1687
                     /locus_tag="JN691_004247"
     CDS             1241..1687
                     /locus_tag="JN691_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAY7245470.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPITSGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(1644..>2422)
                     /locus_tag="JN691_004248"
     CDS             complement(1644..>2422)
                     /locus_tag="JN691_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7245471.1"
                     /translation="HEAAVAFCIHAGTDELTSPVHQHRKGQLILALHGAITCTEENAL
                     WMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTCCTLKISPLCRELILT
                     LANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKIRTMVEMMAKGPVEWG
                     SLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQGLVKGDTVQKVAHTLG
                     YDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
BASE COUNT          598 a          597 c          591 g          636 t
ORIGIN      
        1 ttaacccctg aactggagca gcggttgcag aaactggtgc aatccggttc ttcggtgatg
       61 tttattgcaa ttgacatgga caagttaaag caaataaatg acaccctcgg tcatcaggag
      121 ggggatttag cgattacgtt attagctcag gcgattaaac aatcgattcg taaaagtgat
      181 tatgccatcc gactcggtgg cgatgaattc tgcatcattc ttgtcgattc gacgccgcaa
      241 attgcagcac aactgcctga acgtatcgaa aaacgtctgc aacatatcgc gccgcagaaa
      301 gagatcggct tctcttccgg tttttatgcg atgaaagaaa acgatacgtt acatgatgcg
      361 tataaagctt ccgatgagcg tttatatgtc aataagcaga acaaaaacag ccgttcatga
      421 taaccttctg tggttgtttg cttgtaatct caggagcgtg aaatgactga aatggctaaa
      481 ggaagcgtga cgcatcagca gttaatcgct ttattatcac aagaaggtgc taacttccgc
      541 gtcgttaccc atgaagcggt aggtaaatgt gaagcggtgt cagaaattcg tggcaccgct
      601 ctaggccagg gtgctaaggc actggtatgt aaagtcaaag gcaatggcgt aaatcaacat
      661 gttctggcaa tcctcgcagc cgatcaacag gccgatctga gccaactcgc cagccatata
      721 ggcggattac gcgcttcgtt ggccagcccg gcggaagttg atgaactcac aggctgtgtc
      781 ttcggcgcga tccccccttt cagcttccat ccaaaactca aactggttgc cgaccctcta
      841 ctcttcgaac gatttgatga aatcgccttc aatgcaggga tgctggataa atccgttatt
      901 ttgaaaaccg cggattattt gcgcattgcg caaccagaac tggtcaattt ccgccgcact
      961 gcgtagctag cctgtccgtt caataagcag aacggacaaa accagcacaa ccgcaatagc
     1021 aaaaaacgat gatgtgataa tcagtgtctc tacgaacatt tgatcgttca tagcatgccc
     1081 ccgggagata gctgttttca cgttattgtt agcgtgcaca aatggcagtt tgatgacagt
     1141 tcgcctgatt ttttatgcaa aaaacgtaaa gattttctac ttccttcctg cagcaagcgt
     1201 aaagtaagca ggcttattat tttttggcaa ggaaaccacg atgtttgatg tcactttgct
     1261 gatcctgctc gggttagctg cgctgggctt tatcagtcat aacaccactg tcgccgtttc
     1321 aattctggtg ttgatcattg tccgcgtcac accgttaagc acctttttcc cgtggattga
     1381 aaaacagggg ctaagtatcg ggataatcat tctgacgatt ggcgttatgg cccccatcac
     1441 cagcgggacg ctaccgccct caaccttgat tcactcgttt ctgaactgga aatcgctggt
     1501 ggcaattgca gtaggcgtta ttgtctcctg gctgggtggg cgcggcgtga cgttaatggg
     1561 cagccagccg caactggttg ctgggctgct ggtgggcact gttttaggcg tagcgctgtt
     1621 tcgcggcgta ccggttggcc cgcttattgc cgcgggtctg gtttcgctga ttgtggggaa
     1681 acagtagtta atcccgcaat atatcggcct ggcgtttgcc ccaggccttt cttaaacata
     1741 gtaataaagg ccgttgttga atcatacccc agcgtatgag caactttctg tacagtatcg
     1801 cctttcacta atccctgaag cgccataatc agctgtaact gctgacgcca ctggcgaaaa
     1861 ctcaaccccg tctctttcac tatcagtcgg gcaagattgc gttcactcat cgcaaaaaag
     1921 ccagcccatt gccccaatga cccccactcg acgggccctt tcgccatcat ctccaccatc
     1981 gtgcggattt taggatgaga agaaacgggc aggtgcagct gttgttgtgg ctgctgaggg
     2041 agttcatcaa acaggacctg aattaatctg cgggtcatcg gctcagcacg ctgagtcgtc
     2101 gtcctgtttg ccagggttaa aatcaattcc cggcataagg gcgatatttt caatgtgcaa
     2161 catgttgtcg gcattgtcac tgcgctgggt tcgataaaca gaaaacagag ttcagcgtta
     2221 gctgtcactt gattgctatg cttcaccccg cccggtatcc aaaccgcata ttgtggcggc
     2281 accatccaca aagcattttc ctccgtacag gtaattgcac catgtaatgc tagtatcagc
     2341 tgtcctttgc ggtgctgatg tacaggactg gttaactcat ccgttccggc gtgaatacaa
     2401 aatgcgaccg cggcttcatg gt
//
LOCUS       DADLSA010000287         2410 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.292, whole genome shotgun sequence.
ACCESSION   DADLSA010000287 DADLSA010000000
VERSION     DADLSA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.292"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            5..1138
                     /gene="blaEC"
                     /locus_tag="JN691_004249"
     CDS             5..1138
                     /gene="blaEC"
                     /locus_tag="JN691_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY7245472.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1227..1760
                     /locus_tag="JN691_004250"
     CDS             1227..1760
                     /locus_tag="JN691_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY7245473.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1757..2074)
                     /locus_tag="JN691_004251"
     CDS             complement(1757..2074)
                     /locus_tag="JN691_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY7245474.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2250..2396)
                     /gene="ecnB"
                     /locus_tag="JN691_004252"
     CDS             complement(2250..2396)
                     /gene="ecnB"
                     /locus_tag="JN691_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY7245475.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
BASE COUNT          614 a          634 c          597 g          565 t
ORIGIN      
        1 ccttatgttc aaaacgacgc tctgcgcctt attaattacc gcctcttgct ccacatttgc
       61 tgcccctcaa caaatcaacg atattgtgca tcgcacaatt accccgctta tagagcaaca
      121 aaagatcccc ggtatggcgg tggcggtaat ttatcagggt aaaccttatt actttacctg
      181 gggctatgcg gacatcgcca aaaagcagcc cgtcacacag caaacgttgt ttgagttagg
      241 ttcggtcagc aaaacattta cgggcgtgct tggtggcgac gctattgctc gaggggaaat
      301 caagttaagc gatcccgcaa caaaatactg gcctgaactt accgctaaac agtggaatgg
      361 gatcacacta ttacatcttg cgacctacac cgctggcggc ctgccattgc aggtgccgga
      421 tgaagtgaaa tcctcaagcg acttgctgcg cttctatcaa aactggcagc ctgcatgggc
      481 tccaggaaca caacgtctgt atgccaactc cagtatcggt ttgttcggcg cactggctgt
      541 gaagccgtct ggtttgagtt ttgagcaggc gatgcaaact cgtgtcttcc agccactcaa
      601 actcaaccat acgtggatta atgtacctcc cccagaagaa aagaattacg cctggggata
      661 tcgcgaaggt aaggcagtgc atgtttcgcc aggggcgtta gatgctgaag cttatggtgt
      721 gaagtcgacc attgaagata tggcccgctg ggtgcaaagc aatttaaccc cccttgatat
      781 caatgagaaa acgcttcaac aagggataca actggcacaa tctcgctact ggcaaaccgg
      841 cgatatgtat cagggcctgg gctgggaaat gctggactgg ccggtcaatc ctgacagcat
      901 cattaacggc agtgacaata aaattgcact ggcagcacgc ccggtaaaag cgattaagcc
      961 cccaactcct gcagtacgcg catcatgggt acataaaacg ggggcgaccg gcggatttgg
     1021 tagctatgtc gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg ctaacaaaaa
     1081 ctatcccaat ccagcgagag tcgccgccgc ctgtcagatt ctcaacgctc tacagtaaaa
     1141 tttcatcggg tccgaatttt cggacctttt ctgcgctttt ccttgctgtc atctacactt
     1201 agaaaaaaac cagtaaggaa acatttatgc gcctgctccc tctcgttgcc gcagcgacag
     1261 ctgcatttct ggtcgttgcc tgtagttctc ctacgccgcc gcgtggcgtg accgtagtga
     1321 ataatttcga cgccaaacgt tatctcggta cctggtatga gattgcccgt tttgatcacc
     1381 gttttgaacg tggactggaa aaagtcaccg caacatacag cctgcgtgat gacggcggcc
     1441 tgaatgtcat taataaaggc tataaccctg acagaggaat gtggcagcag agtgaaggga
     1501 aagcgtactt taccggcgca ccaactcgcg ctgcgctgaa agtgtcattc tttggtcctt
     1561 tctatggcgg ttataacgtt attgcactcg atcgggaata ccgccatgcg ctggtttgcg
     1621 gcccggaccg cgactacctg tggatactct cccgcacgcc aaccatttct gacgaagtga
     1681 aacaggagat gctggcagtc gcgacccggg aagggtttga tgtcagtaag tttatttggg
     1741 tacagcagcc cggtagttag tgagtgctga gtttcagacc aataatcccc aatacgatta
     1801 acgccagact cgccagacgc atcgggttag cggactcacc gagcagcaca atgccggtga
     1861 tggccgcgcc gactgcgcca atacccgtcc acacggcata agccgtccct accggtaacg
     1921 atttcatcgc ccaggcaagt agcgccatac tgactatcat cgccgtcacg gtaataacac
     1981 tcggcgtcaa acggctaaag ccgtgggtat atttcaggcc aacggcccat accacttcca
     2041 gcagaccagc aataactaag ataatccagg acatatcagg ctccggaaat ttggggccgt
     2101 ccccggtgaa aagaggcgtt tgcaggtcgt cctgcaaagc tgatggttga acggatattt
     2161 tgccaggtgg caggggaatt tcaactatta cctttatgac gatcgcagaa aaaacaaacg
     2221 gcacgacact gctgtcgtac cgttattgct tattgctgcg ctttcgttgc tgcgccagaa
     2281 atcgcgttac cgccatcaga aatgtcttca ccaacgccac gcgtggtgtt gcaggcagtt
     2341 aatactgttg aaagcaccag aacagaaaag atcgctgcaa ttgtcttctt caccataacg
     2401 tcttcctttt
//
LOCUS       DADLSA010000288         2385 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.293, whole genome shotgun sequence.
ACCESSION   DADLSA010000288 DADLSA010000000
VERSION     DADLSA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.293"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            329..2385
                     /gene="tssH"
                     /locus_tag="JN691_004253"
                     /pseudo
     CDS             329..2385
                     /gene="tssH"
                     /locus_tag="JN691_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459269.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
BASE COUNT          565 a          565 c          662 g          593 t
ORIGIN      
        1 gtatcactgt gtagagtgtt tctttcaaaa gaataagttt tctaaatttt tagataagca
       61 atgctgatca atttacttta tcaatataag ttttcgcgga aatatatatg gcgattttta
      121 atttaatata ttcatggtgt gtatgaaatt ggcttactct attgaatatt accatgaaaa
      181 agatactacc tgctatatta cattgtgttc tgtgatatgc atcattgttt cattctgcct
      241 ttttgataat tctattaatt agaagtttta atcggtacag ttatgtttat gtatcattta
      301 tatgttatta catcatcagg tgtaacagat ggacactcct gtttcgcgca gtgcactata
      361 tggaaagcta accaaatctt tgtttagatc tttggaatct gcgacaacgt tctgtaaatt
      421 acgcgctaat ccttatgtcg aactgacgca ttggttgcat cagttaatac aacaaccaga
      481 gaatgacatt cacaatgtgc ttcgacatta tcaaataaca tctgctgatg ttgagaaggc
      541 gttaattcag caattgaata tattgcctgc cggagcgagt gtaattaacg atttttccca
      601 tcatatcgat ctttctgttg aaaaagcctg gatgttggca agtatccgct ttggcgatca
      661 aaaaattcgt agtggatggt tattacttgc ctggttaacc acgccagaat tgtgtcgggt
      721 gttaaaaaat atttgtgcgc cattggcaga gctaccggta gatgaactat tagatattct
      781 tccgtcttta atcacgtcat ctcctgaatt aacagaaatg ccttatgacg gctcggattt
      841 aaattctgca gtaccggggg aagctagcca ggtctttgcc ggcgaatcaa ctggaagcaa
      901 atccgcgctg gcgaaatact gtcaggacat gactgcgctg gcccgtgaag gcaatatcga
      961 tccggtcacc ggtcgcgaac acgagatccg tactatgacg gatattctgc tgcgccgtcg
     1021 ccagaacaac ccgctactga ccggcgaagc gggagtcggg aaaacggcag tggtggaagg
     1081 attcgcgctg gcgattgccg ggggggaagt accgccttca ctgcgtaacg tgcggttgct
     1141 gtcactggat gttggcgcgc tgctggctgg ggcgtccatg aagggtgaat ttgaatcccg
     1201 cctgaaagcg ttgctggaag aggccgcgca ctcgccgcag ccggtaatcc tttttgttga
     1261 tgaagttcac acgctggtgg tacatccggt acgggggatg ctgccaacct gctgaaaccg
     1321 gcgctggcgc gtggcaccct gcgcaccatc ggcgcaacca cctggagcga gtacaaacgc
     1381 catatcgaaa aggatccggc gctgactcgg cgttttcagg tattgcaggt tgctgagccg
     1441 gaagaggttg cggctgttga gatggtacgc gggttagtca ccacgcttga aaaacaccat
     1501 gacgtgctga tccttgatga ggccgtgcgc gccgccgtac agctttctca ccgttacatt
     1561 ccggcccggc aactacccga taaggccatc agcctgctgg atacggcggc ggcccgtgta
     1621 gcgctgtcgc tgcatacccc gcctgcgaga gtccagtatc tgcgccagca gctcaacgct
     1681 gccgaactgg agctggagtt gttattaaag caggaaaaaa tgggtatccg ctcggagcgg
     1741 ggcgatgtac tggcggcgcg gattatatcg ctgaatgccg atctttctga caccgaagcg
     1801 cggtggcagc aggaactgga actggttcac cgattacagg aacagcgtgc cgcagaaccc
     1861 ggcgagcgtg acgaaatcac gctgcaacag gcagaaacgg cattaagaga ctggcagggc
     1921 gaaaaccccg tcgtgttccc ggaagtcagc gcggcagtgg tggccgcaat tgtttctgac
     1981 tggaccggta ttcccgccgg acgcatggtg aaagatgaag ccagtcagat cctggaactg
     2041 cccgcccgtc tggcgcagca tgttaccggg cagagggacg cgctggcgca gattggcgag
     2101 cgtatccaga ccgcgcgggc gggtttaggt gaccagcgta agcctgtggg cgtctttttg
     2161 ctggccgggc cgtccggtgt tggcaagact gagaccgctc tggcgctggc agaggctatc
     2221 tatggcggtg agcagaacct gatcaccatc aacatgagcg agtttcagga gtcccacacc
     2281 gtttccacgc tgaaaggcgc accgcccggc tacgtgggtt atggcgaagg cggcgtgttg
     2341 acagaagcgg tacgccgcca tccctggagc gtggtattgc tggat
//
LOCUS       DADLSA010000289         2313 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.294, whole genome shotgun sequence.
ACCESSION   DADLSA010000289 DADLSA010000000
VERSION     DADLSA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.294"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..517
                     /locus_tag="JN691_004254"
     CDS             <1..517
                     /locus_tag="JN691_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7245476.1"
                     /translation="VDNIDGKSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENKES
                     LFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWKSLEFKRSDH
                     SLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAINDYG
                     GSSGTETRPLQ"
     gene            546..>2313
                     /locus_tag="JN691_004255"
     CDS             546..>2313
                     /locus_tag="JN691_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7245477.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVLGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          691 a          544 c          503 g          575 t
ORIGIN      
        1 ggtagataat atagatggta aaagccgtgc cccatttatt ataaccccac cgctatttcg
       61 tcttgaggct ggcgatgact catcactgcg aattattaaa acagctgata acctgcctga
      121 aaataaagag tcgctgttct acattaatgt tcgtgccatt ccagcaaaga aaaaatcaga
      181 tgatgttaat gctaacgagt tgacgctggt atttaaaaca cggatcaaaa tgttttatcg
      241 ccccgcacac ctgaagggac gggtaaacga tgcgtggaaa tcactggaat ttaaacgtag
      301 tgaccattca ctcaatatat ataacccaac tgaatattac gtcgtatttg ccggactggc
      361 agtcgataaa accgatctca caagcaaaat tgaatatatc gcgcccggag aacataaaca
      421 gttaccactt ccagcatctg gcggaaagaa cgtgaaatgg gctgcgatca acgattatgg
      481 cggcagttcc gggacagaaa ctcgtccact gcaataaaaa atataaaaac acaggtcatc
      541 agggaatgcc tcaacgacac caccagggac ataaacgcac accgaaacag ttggcgctca
      601 ttatcaaacg ttgtttgccg atggtgctca ctggcagcgg catgctttgc actaccgcta
      661 acgccgaaga gtattatttc gaccccatta tgctggaaac tacaaaaagt ggtatgcaaa
      721 caaccgatct gtcacgtttt tcaaaaaaat acgcacaact accaggaact tatcaggttg
      781 atatctggct gaataaaaag aaggtttcac agaaaaaaat tacatttacc gccaatgcag
      841 agcaacttct gcagccacag tttacggtag aacaactacg tgagctgggt attaaggtgg
      901 atgaaatccc ggcgctggct gaaaaagatg acgatagcgt gatcaactcg cttgaacaaa
      961 tcattcccgg tacagctgct gaatttgatt tcaatcatca gcgacttaat ttgagcattc
     1021 cccaaattgc actgtaccgt gatgcaagag gttacgtctc cccttctcgt tgggacgatg
     1081 gtataccaac gctgtttacc aactactcgt ttacaggttc tgataaccgt taccgccagg
     1141 gcaatcgtag ccaacgacag tacctgaata tgcaaaatgg tgctaatttt ggcccctggc
     1201 gattacgtaa ctattctacg tggacacgca acgatcaggc gtcaagctgg aacactatca
     1261 gtagttattt acaacgtgat atcaaggcgt tgaagtctca gttgcttctg ggagaaagcg
     1321 ccaccagcgg cagtattttt tccagctaca cctttactgg cgtgcaactc gcttccgacg
     1381 ataatatgtt gccaaacagc cagcgcggat ttgccccaac ggtactcggt atcgcaaaca
     1441 gtagtgcaat cgtgactatc aggcaaaatg gttatgtgat ctatcaaagc aacgtgccag
     1501 cgggtgcctt tgaaattaac gatctctacc cctcttccaa cagcggcgat ttagaagtca
     1561 cgattgaaga aagtgacggt acacaacgtc gctttatcca gccttattct tcattaccca
     1621 tgatgcagcg acctgggcat ctaaaatata gcgcgaccgc tggacgctat cgcgctgatg
     1681 caaacagtga tagcaaggaa cccgaatttg ctgaagccac ggcaatatat ggtttgaata
     1741 atacttttac gctgtatggc ggcctgctcg gttctgaaga ttattatgcg ctggggatcg
     1801 gtatcggcgg cacacttggc gcactgggcg cgttgtcgat ggatatcaac agagctgaca
     1861 cccaattcga taaccagcac tcttttcatg gctatcaatg gcgtacgcag tacatcaaag
     1921 atatcccgga aaccaacacc aatatcgctg ttagctacta tcgctatacc aacgatggct
     1981 attttagttt tgatgaagcc aatacccgca attggggcta taacagtcgc caaaaaagtg
     2041 aaattcaatt caacatcagc cagacaatat ttgatggggt aagtctgtat gcctccggtt
     2101 cacagcaaga ctattggggc aataacgaga aaaacagtaa tatctctgtt ggggtttccg
     2161 gccagcaatg gggaattggt tacagcctga attatcaata cagccgctac actgatgaaa
     2221 ataatgaccg tgcactctct ttgaatctca gtattccgtt agaacgctgg tggatttgcc
     2281 cctatatttc cagacatctg ttatcactta acc
//
LOCUS       DADLSA010000290         2258 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.295, whole genome shotgun sequence.
ACCESSION   DADLSA010000290 DADLSA010000000
VERSION     DADLSA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.295"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(18..1766)
                     /gene="msbA"
                     /locus_tag="JN691_004256"
     CDS             complement(18..1766)
                     /gene="msbA"
                     /locus_tag="JN691_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAY7245478.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(1803..>2258)
                     /locus_tag="JN691_004257"
     CDS             complement(1803..>2258)
                     /locus_tag="JN691_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAY7245479.1"
                     /translation="SAHWPLQGSNDKGNNHSCVVKVDDGTNSILLTGDIEAPAEQKML
                     SRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKVALASASRYNAWRLPSNKVKHRYQ
                     QKGYQWLDTPHQGQVTVNFSAQGWRISSLREQILPRWYHQWFGVPVDNG"
BASE COUNT          585 a          590 c          533 g          550 t
ORIGIN      
        1 agaccagatt ttttcgatca ttggccaaac tgcattttgt gaagttgcgc gtaaacgccg
       61 cggtgctcaa gcaaatcgtt atgcgtaccg cgttccacaa tgacaccatc ctcgacgacc
      121 acgatttcgt ctgccttttc aatggtagac aagcggtggg caatcaccag agaggtacgg
      181 tttttctgca actcatccag tgccgcctga atcgcacgtt cggattcggt atccagagcc
      241 gaggtagctt catccagaat cagtatcggg ctatcacgca gcaaggctcg agcgatagca
      301 atacgctgac gctgaccgcc agagagcagc acgccgtttt caccaatcac tgtatcgaga
      361 ccgttatcca tcttattgat gaagtccatg gcgtaggcca tacgcgccgc ttcttcaatt
      421 tgctcacggc tgtactgttc agtccgtgcg taagcaatgt tgttagcaac cgtatcgtta
      481 aacagatgga cattctgcga caccagagca acctggttac gtaacgacgc cagggtatac
      541 tcgcgcagat cgtgaccatc catcaggatt tcgccttcat caatatcgta aaaacgcgtg
      601 atcaggctgg cgatggttga tttacctgaa ccagagcgtc caaccagagc aaccgtcttc
      661 cctgccggaa ttttcaggtt gatgttacgc aatgcgggta cgtcacgtcc cggataagta
      721 aaggtgacat tgcggaattc cacgtcgcca gtcgcacgct cgatcacgcg cttaccttcg
      781 tctttctcct gctcactgtc cagaatggta aacagcgtct gacaagccgc cataccgcgc
      841 tggaactggg cgttaacgtt ggtcagcgat ttcagcggac gcatcagtgc aatcattgaa
      901 gagaaaacaa cggtaatcgt accggcagtc aggttatcca taacacttgg gaagctcgcc
      961 gcatacagaa caaacgccag cgccaaagag gcgatcagct gaatgatcgg atcagagatg
     1021 gaagaggctg aaaccatttt catcccctga agacgcattc ggttactgac tttatcgaag
     1081 cgtttcgttt ccacttcctg accaccgaaa atcaatactt ctttgtggcc cttcagcatt
     1141 tgttctgcac tggtggtcac ttgccccatg gtgttctgca tgtttttact gatgttgcga
     1201 aaacgcttcg atactacgcg aatcgcaatc gaaacaatcg gtgccagcac aatcaaaatg
     1261 atcgacagtt gccaactgta atagaacatc atgatgaaca ggccgatgat cgacgcacct
     1321 tcacgcacaa cagtaatcag tgcgccggaa gaagaagaag caacctgttc ggaatcgtag
     1381 gtgatacgtg acaacagcgt acccgttgac tgtttgtcaa agaatgaaac tggcattccc
     1441 atcatgtgac caaacaggcg gcgacgcatg gtcattacca cctttcctga tacccaggag
     1501 atacagtagc tggagacata gctggtgata ccacgtaaaa tcatcagtcc gatcaccacc
     1561 agcggcatcc acaccagcac ggagcgatct gttttaccaa agccatcatc aagaagtggc
     1621 ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga ggattaacgc tacgcccgcc
     1681 acgatcagac ccgctttgaa aggcgcaatg gttggccaca gtcggcggaa tgtctgccac
     1741 gtagagagat ctttgtcgtt atgcattcaa aaaaccagca tttgttgaaa tagccgcata
     1801 ttctacccgt tatccactgg cacgccaaac cactgatgat accaacgagg taaaatttgc
     1861 tctctgaggc tgctaatccg ccagccttgt gctgaaaaat tgaccgtcac ttgaccctga
     1921 tgtggagtat caagccattg atatcctttc tgttgatagc gatgcttaac tttgttagag
     1981 ggcaatcgcc atgcgttata gcgcgatgcc gatgcgagtg ccacttttcc attcactcgc
     2041 tgaattaatg gcaacgatga tgaggtatta ctgccatggt gaggtacctg aagcaatgtt
     2101 gcctgcactt gctgccagta acggcttagc atcttttgtt cagctggggc ttcaatatca
     2161 ccggttagaa gaatgctatt cgtcccgtca tcaaccttaa ccacacagga atggttattt
     2221 cccttatcgt tgctaccttg taaaggccag tgcgcgct
//
LOCUS       DADLSA010000291         2245 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.296, whole genome shotgun sequence.
ACCESSION   DADLSA010000291 DADLSA010000000
VERSION     DADLSA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.296"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            11..565
                     /locus_tag="JN691_004258"
     CDS             11..565
                     /locus_tag="JN691_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7245480.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            667..1158
                     /locus_tag="JN691_004259"
     CDS             667..1158
                     /locus_tag="JN691_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAY7245481.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1222..1997)
                     /locus_tag="JN691_004260"
                     /pseudo
     CDS             complement(1222..1997)
                     /locus_tag="JN691_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(2024..>2245)
                     /locus_tag="JN691_004261"
     CDS             complement(2024..>2245)
                     /locus_tag="JN691_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="nucleator protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HAY7245482.1"
                     /translation="SALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIVDIANRDG
                     QLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
BASE COUNT          522 a          531 c          591 g          601 t
ORIGIN      
        1 aaatatatta atgaacgagt tttctatcct ctgtcgtgtg ctgggttcgc tctattaccg
       61 ccaaccgcaa gatcctttac tggtgccgct gtttactctg attcgtgagg ggaaactggc
      121 tgcgaactgg ccactggagc aggatgagtt actgacgcgt ttacagaaaa gctgtgatat
      181 gacgcaagtc tctgccgatt acaatgcgtt gtttatcggc gatgaatgtg ccgtgccgcc
      241 atatcgtagc gcatgggttg aggacgcaac ggaagcggaa gtgcgcgctt ttctttccga
      301 acgagggatg ccactggcgg atacgccagc cgatcacatc ggcacattgc tgcttgcggc
      361 ttcctggctg gaagatcagt caacggaaga tgagagcgaa gcactggaaa cactgttcag
      421 tgagtatctg ttaccctggt gtggtgcgtt cctcggcaaa gtggaggccc atgcaaccac
      481 gcctttctgg cgcaccatgg caccgctaac ccgtgatgcc attagtgcaa tgtgggacga
      541 gctggaagaa gattctgaag agtaaatgtg atcatcgtca ctcataactg caacagataa
      601 tttgttgttg cataaaatgt gtgctcgatc tcattcatgg ccgcgttttc tgctatcatg
      661 cgcggcatga acatacttct ctctattgca atcacaacag gcattctctc cggtatctgg
      721 ggatgggtgg ctgtttccct gggcttacta agctgggcgg gcttcctggg ctgtacggcc
      781 tactttgctt gcccgcaagg tgggctgaaa gggctggcga tctccgctgc aaccctgctt
      841 agtggcgtgg tgtgggcgat ggtcattatt tacggtagtg cactggcacc acatctggaa
      901 attctcggtt atgtcataac cggtattgtc gcgtttctga tgtgtattca ggccaaacag
      961 ctgctgcttt catttgttcc ggggacgttt ataggcgcat gcgcgacgtt tgccgggcag
     1021 ggtgactgga aactggtgtt accttcgctg gcgcttgggc tggtatttgg ttacgcaatg
     1081 aaaaacagcg gcctgtggct ggcggcgcgt agtgcaaaga cggctcaccg tgagcaggaa
     1141 atcaaaaata aagcgtgagg ggcactcacg ctttcgctta aacagtaaat gccggattat
     1201 taattccggc atttttatct gtcaggattc cggtggaacc gacatatggc ggtatttcac
     1261 cagaatgtca ttctgccgtt ctgctttatt ttgcaaatcc cacagaccac ggtcgatacc
     1321 atcattaatc aggaaaatga cccctgtttc gatagccgac atcagacaca gcataacggg
     1381 ttcgtttgag gtgtaaccca cttccccttc cagcaagcgc tggtagtcaa taaagcggaa
     1441 aaccccggcc tgaacttcat aggaaagtat cgtcttactg gtgttcaccg aggagaggat
     1501 ctcaccggta ctcacattga cgacacgcag gttcacggca atctgatcga gctggtattg
     1561 cgtgtctgca ccgatcccaa aatatcttgc cccaacaccg cccgatttaa cgttgctttc
     1621 ataaccgata atcgaacctt caaccataat atttgccgcc gttaaagatt gcagcgggat
     1681 tcggttatta atggcaaccg tgccgttttc ttgtgccgca cgaataatct tgcgctcgtt
     1741 aagcaggttt tgtaagccct ggcgctttgc ggaacagcag tggagaagtt acttgccggg
     1801 tagggtttaa attgcctggt ttcgtcctga atgttgtata ccgaaacaaa gatcttaccc
     1861 gtcggcgctg gcagatgggt caaatctttg tagctctgag cacgaggcat taatgtcggt
     1921 ctggcggctt ctttaggcgg ggcggttaag catccgctca gtaacatgac ggcaaccaaa
     1981 agaaataagc gctgcatgat tattttcctt atgaagctgg ggcttaaaaa tcggttgagt
     2041 tattttgtaa acccgaaacc tggatggtcg aggtttgtcc ggttttacga tctgtcacgt
     2101 tcaactgcaa ttgaccatcg cggttagcaa tatcaacaat ataatcgttg gtcaccatgc
     2161 ggcccggttt accggtatta atattcgaca gtagcccacc taaaatttgt gactggatgg
     2221 cctgagtaaa gttatctaac gctga
//
LOCUS       DADLSA010000292         2227 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.297, whole genome shotgun sequence.
ACCESSION   DADLSA010000292 DADLSA010000000
VERSION     DADLSA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.297"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..1026
                     /locus_tag="JN691_004262"
     CDS             <1..1026
                     /locus_tag="JN691_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HAY7245483.1"
                     /translation="LLPFAHELRKRGVNNRIGFFLHIPFPTPEIFNALPTYDTLLEQL
                     CDYDLLGFQTENDRLAFLDCLSNLTRVTTRRAKSHTAWGKAFRTEVYPIGIEPKEIAK
                     QAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLAYEALLEKYPQHHGKIRY
                     TQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQHFDRKLLMKIFRY
                     SDVGLVTPLRDGMNLVAKEYVAAQGPANPGVLVLSQFAGAANELTSALIVNPYDRDEV
                     AAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQIVPRSAESQQRDKV
                     ATFPKLA"
     gene            complement(1033..1461)
                     /gene="uspC"
                     /locus_tag="JN691_004263"
     CDS             complement(1033..1461)
                     /gene="uspC"
                     /locus_tag="JN691_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HAY7245484.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT          624 a          479 c          486 g          638 t
ORIGIN      
        1 ctgttgccct ttgcgcatga attacgcaaa cgtggggtga ataatcgcat tggttttttc
       61 ttgcatattc ctttcccgac accggaaatt ttcaacgcgc tgccgacata tgacaccttg
      121 cttgaacagc tttgtgatta tgatttgctg ggtttccaga cagaaaacga tcgtctggcg
      181 ttcctggatt gcctttctaa cctgacccgc gtcacgacac gtcgcgcaaa aagccataca
      241 gcctggggaa aagcatttcg aacagaagtc tacccgatcg gcattgaacc gaaagaaata
      301 gccaaacagg ctgccgggcc actgccgcca aaactggcgc aacttaaagc ggaattgaaa
      361 aacgtacaaa atatcttttc tgtcgaacgg ctggattatt ccaaaggttt gccggagcgt
      421 tttctcgcct atgaggcatt gctggaaaaa tatccgcagc atcatggtaa aattcgttat
      481 acccagattg caccaacgtc gcgtggtgat gtgcaagcct accaggatat tcgtcatcag
      541 ctcgaaaatg aagctggacg aattaatggt aaatacgggc aattaggctg gacgccgctt
      601 tattatttga atcagcattt tgaccgtaaa ttactgatga aaatattccg ctactctgac
      661 gtgggcttag tgacgccact gcgtgacggg atgaacctgg tagcaaaaga gtatgttgct
      721 gctcagggcc cagccaaccc gggcgttctt gttctttcgc aatttgcggg agcggcaaac
      781 gagttaacgt cggcgttaat tgttaacccc tacgatcgtg acgaagttgc agctgcgctg
      841 gatcgtgcat tgactatgtc gctggcggaa cgtatttccc gtcatgcaga aatgctggac
      901 gttatcgtga aaaacgatat taaccactgg caggagtgct tcattagcga cctaaagcag
      961 atagttccgc gaagcgcgga aagccagcag cgcgataaag ttgctacctt tccaaagctt
     1021 gcgtaggagc ggttaatctc ccgtaagtgg aactaacagc acatcgacct cacttgaggc
     1081 aatcactctt ttcgccgagc aggatgctcg cgaaaagaaa ctgtgattgt gattaccgca
     1141 aatcaccaaa ttgaaatgat gtttgtgaca tacttccaga atatgttcgc ttaactcacc
     1201 gtaggcaata aacgtcttgt caacgggata ccccgcatcc tgaattaact tatcaagaaa
     1261 gctttgcgtt tcttcttgca tcacgctgcg taaatcttca agcatcggcg cggctaattg
     1321 attgtacatt tccgggtcgg aagcgagagt aattaaactg atgtgtccct ttactggcct
     1381 ggcgatagat accgcttttg ccagcagttg ctgactttcc ggtgtaaccg caacagcgac
     1441 aagaatattg ctatagctca taacctgttc cttattctgt gaacttcagg tgacattaaa
     1501 gctttactcc taatggagaa acgacgcaat cccaactcag tcaaacaggt aaatattagt
     1561 ctgattatta gtaaagacaa ttaatcaatc actctcggtg atcatatgat ttcaactgta
     1621 attataagaa aaaaatgtac tgattccccg cattaaacaa gtggctaata tatgaaaaac
     1681 attttattaa cattaagttg attgttgcct ttctttgtat ttaattagtt tgttgtgcgg
     1741 taagtgtctg tttaaaaata gcttatctgg tattgcatga aagtgattat ttatagcaga
     1801 tgattattta cggtgagtta ttttaaccgt gcgcaacatc ccatttcgat tattcctgtt
     1861 tcatttttgc ttgctagcgt agcgaaaaac tttttaacag attgaaatac acccaaaaca
     1921 aaagtatgac ttatacattt atgttaagta attgagtgtt ttgtgtgatc tgcatcacac
     1981 attattgaaa atcgcagccc ccctccgttg tatgtgcgtg tagtgacgag tacagttgcg
     2041 tcgatttagg aaaaatctta gataagtgta aagaccccct tctatttgta aggacttatt
     2101 aaaccaaaaa ggtggctctg cttattgcag cttatcgcaa ctattctaat gctaattatt
     2161 ttttaccgga gcttcccggc gacatcacgg ggtgcggtga gaccgcataa aaataaagtt
     2221 ggttatt
//
LOCUS       DADLSA010000293         2222 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.298, whole genome shotgun sequence.
ACCESSION   DADLSA010000293 DADLSA010000000
VERSION     DADLSA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.298"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            125..1098
                     /locus_tag="JN691_004264"
                     /pseudo
     CDS             125..1098
                     /locus_tag="JN691_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1095..2186)
                     /locus_tag="JN691_004265"
     CDS             complement(1095..2186)
                     /locus_tag="JN691_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7245485.1"
                     /translation="MFKIFKIPVNKWTISTAALGGIFIVSGLILLMNYNHPYTFKAQK
                     AVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQARVDRLMADIVTAEHKQ
                     RALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFSERDIDVARQNYLAQE
                     ASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLEQTIVRAPSDGYVTQV
                     LIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGDDAEVVFNALPGKVFS
                     GKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHTDLSALPDGIYAQVAV
                     YSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
BASE COUNT          584 a          558 c          515 g          565 t
ORIGIN      
        1 tgtccggtat tccattttaa aataagataa aaaagagtcg gcataataat gcttacaatt
       61 tctccggtgc aagattcata tttatatagt aatatataaa attatatata attgggctgt
      121 tgcgatgaca aaattacaac ttaagtaccg ggagttaaaa attatctcgg taatcgctgc
      181 cagtgaaaat atcagccatg ccggactgta cttggcatcg cacaggccaa cgtcagcaaa
      241 tatcttgctg attttgaatc aaaagtgggt ttaaaagtct ttgaccgaac cacccggcag
      301 ctgatgctca caccttttgg caccgcgctg ttaccgtaca tcaacgacat gctggacaga
      361 aacgagcaac ttaataattt tattgcagat tataagcatg agaaacgtgg tcgggtcacc
      421 atctacgccc caaccggcat aattacctat ttatccaaac atgtaatcga taaaattaaa
      481 gatatcggtg atatcacctt atcgctaaaa acctgcaatc ttgagcgtaa tgccttttat
      541 gaaggcgtcg aatttcctga tgattgtgat gttttaatca gctacgctcc accaaaagat
      601 gaatcgctgg ttgccagttt tattacacaa tatgccgtaa ccgcttatgc cagccagcgc
      661 tatcttgaga aacatcctat tagccgtcct gacgaactcg aacatcactc ctgtattttg
      721 atcgactcga tgatgatcga cgacgcgaat atctggcgct tcaacgtcgc cgggagtaaa
      781 gaggtgcggg attatcgcgt gaagggaaat tacgtttgcg ataacacgca atcggcgctg
      841 gagctggcac gaaatcacct ggggattgtg tttgcgccag acaaaagtgt gcagagcgat
      901 ctgcaagacg gcacgctggt gccctgcttt cagcacccgt atgaatggtg gctggatctg
      961 gtggctatct tccgtaaacg ggaatacctg ccctggcggg tccagtatgt gctggatgaa
     1021 atgctgcgtg aaattcgcca ccagcttgct cagtcgcagc aactgcggcc tgaacaagcg
     1081 gcggaaagcg aagattaatg atcgagataa aggtaatgca cccagctggt catgcgtaat
     1141 agcactttgc gcatcaccga gacatggctg aaatgatcag agtagaccgc cacctgggcg
     1201 taaataccgt ctggtaaagc gctcaaatca gtgtgctcat ccagttcaat ggtcgcgata
     1261 acgccatctg aacccggcgc tgtgtttaac gtctgtaagg tgccggtcga ctgataagct
     1321 ccgccgggaa cagctggact aatggctgcc agcttgccgc tgaatacctt acctggcaga
     1381 gcattaaaca ccacttccgc atcgtcgcca ggggccagtc gcagcaagga gttctgacgg
     1441 aactgcgcca caatttgtcg tttctgatcg ggtataaaca ccatcaccgg acgtagcggc
     1501 agcgacgcgg cataggtacc cggacgaatc agcacctggg tcacataacc atcgctcggc
     1561 gcacgcacta tcgtctgctc aaggttatat tttgcttccg cgagctgcgc tttcaggctg
     1621 gcgatttgag aatgttcacc caacaccagg ctatccagct ggctctggat ctgtttttgt
     1681 tccgccgccg atgactttac ggaggcttcc tgcgccagat aattttgccg cgccacatca
     1741 atatcgcgtt ctgaaaacgg gtttactttc gcctggctgc cgcgtgcgta acgctgatac
     1801 tctttagcga atttatcccg cgtggccttt gcctgctgag tattcgccgc catctcatct
     1861 aactctgcgc ccaacgcccg ctgtttatgt tctgcggtaa cgatatccgc catcagccga
     1921 tccacccgcg cctgataacg cgtcgggtcc agtcgaaata gcacctcacc ttttttaatc
     1981 agcgtatttt tcttatccgt cacttcgatc accacgcctg tcacctgtgg gacaacagga
     2041 atagaaataa ccgctttttg cgctttgaag gtatacggat ggttatagtt cattaacaga
     2101 attaaaccac tgacaataaa tatgccaccc agggccgctg tggaaatggt ccatttattt
     2161 acaggaattt tgaagatctt aaacattgcc catgcaaaag ccacataggt caaaataatc
     2221 ag
//
LOCUS       DADLSA010000294         2169 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.299, whole genome shotgun sequence.
ACCESSION   DADLSA010000294 DADLSA010000000
VERSION     DADLSA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.299"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            208..1923
                     /locus_tag="JN691_004266"
     CDS             208..1923
                     /locus_tag="JN691_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAY7245486.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            1920..>2169
                     /locus_tag="JN691_004267"
     CDS             1920..>2169
                     /locus_tag="JN691_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY7245487.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNIST"
BASE COUNT          461 a          558 c          564 g          586 t
ORIGIN      
        1 gtttcgtttt ttacatctga actttgcaat tttccattca ttttaatatc cctccggcga
       61 attatgctca caacctgaac ttaacagaaa acaatccgtt cagggatacg acgtttgtat
      121 tttaagaatc tgactgcctg acccgacgca ttttacctct ccctatattc atgcgtccgg
      181 gacataaaca taaataaggg ctatgagatg aattcgttac aaatcttgag ttttgtcggt
      241 tttacgctgc tggtggcggt gatcacctgg tggaaggttc gcaaaacaga taccggatcg
      301 caacaaggct attttcttgc cggacgttca ctaaaagcgc cggttattgc cgcttcgtta
      361 atgttaacca acctttccac ggaacaactg gttggtcttt ccgggcaggc ctacaaaagc
      421 ggcatgtcgg tgatgggctg ggaagtgact tcagcggtga cgctgatctt cctcgcgcta
      481 atctttttac cgcgctatct gaagcgcggc attgccacca tccccgattt tctggaggaa
      541 cgttatgata aaacgacgcg tattatcatc gacttctgct tcctcattgc caccggcgtc
      601 tgctttctgc cgattgttct ctactccggc gcgttggcgc tcaacagcct gtttcacgtc
      661 ggggaatcgc tacagatttc tcacggtgcg gctatctggc tactggtaat tttgcttggt
      721 ctggcgggaa ttttgtatgc ggtgatcggc ggactgcgcg caatggcagt ggcggactcc
      781 atcaacggta ttgggctggt tatcggcggg ttgatggtgc cggtatttgg cctgatcgcg
      841 atgggcaagg gcagctttat gcagggcatt gagcaaatta ccaccgttca cgccgagaaa
      901 ttaaactcag tcggtggccc gaccgatccc ttgccgattg gcgcggcatt taccggttta
      961 attctggtga acacctttta ctggtgtaca aatcagggca tcgtgcaacg cacgctggcg
     1021 tcaaaaagcc tggcggaagg gcaaaagggg gcgctgttaa cggcggtgct gaaaatgctc
     1081 gacccgctgg tactggtgct gccagggttg attgcgtttc atctgtatca ggatctaccg
     1141 aaagccgaca tggcctaccc gacgctggtc aataacgttc tgccagtgcc actggtgggt
     1201 ttcttcggcg cggtgttatt tggtgcggtg atcagtacct tcaacggctt tctgaatagc
     1261 gccagtacat tattcagtat gggtatttac cgtcgcatca ttaaccagaa tgccgagccg
     1321 cagcagctgg tcaccgtcgg gcgcaaattt ggtttcttta tcgctatcgt ttcggttctg
     1381 gtagcgccgt ggatcgccaa cgcgccgcag gggctgtata gctggatgaa acagctcaac
     1441 ggcatttaca acgtgccgct ggttaccatc atcattatgg gctttttctt cccgcgcatc
     1501 ccggcgctgg cggcaaaagt ggcgatgggg attggcataa tcagctacat caccatcaac
     1561 tatctggtga agttcgactt ccatttcctc tatgtgctgg cctgtacgtt ctgcatcaac
     1621 gtggtcgtga tgctggtgat cggttttatc aaaccgcgcg ccacgccgtt caccttcaaa
     1681 gatgcgtttg cggtggacat gaaaccgtgg aaaaacgtca agatcgcgtc aattggtatc
     1741 ctgttcgcga tgattggcgt ctatgtcggg ctggctgaat tcggcggcta cggtacgcgc
     1801 tggttagcga tgatcagtta tttcatcgct gccgtagtga ttgtctacct gatttttgac
     1861 agctggcggc atcgtcacga cccagccgta acctttactc ccgacgcgaa ggatagccta
     1921 tgaaacgccc caattttctg ttcatcatga ccgataccca ggccaccaat atggtcggtt
     1981 gctatagcgg taaaccgctg aatacgcaaa atattgatag tctggcggcg gaaggtattc
     2041 gctttaattc cgcctacact tgttcaccgg tttgtacgcc tgcacgcgcc ggactattca
     2101 ccggtatcta cgctaaccag tccggcccgt ggaccaacaa cgtcgcgcca ggcaaaaaca
     2161 tctccacta
//
LOCUS       DADLSA010000295         2106 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.300, whole genome shotgun sequence.
ACCESSION   DADLSA010000295 DADLSA010000000
VERSION     DADLSA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.300"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            1..814
                     /locus_tag="JN691_004268"
                     /pseudo
     CDS             1..814
                     /locus_tag="JN691_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1253..2090
                     /locus_tag="JN691_004269"
                     /pseudo
     CDS             1253..2090
                     /locus_tag="JN691_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          523 a          511 c          632 g          440 t
ORIGIN      
        1 ccagtacacc tcagttctgg gccttgcccg tagcagttat ttttatcacc gggctgcact
       61 gaaagccggt gataaatacg ccacgatacg taagatgctg acagatatct ttaacagtaa
      121 ttaccagtgt tatggctatc gtcacctgca tgcgatgctc aggcatgagg gggggcggct
      181 atcagaaaag gttgtacgca gacttatggt ggaggaacag cttgttgtca gtcgtaaccg
      241 tcgtcgtcgc tacagctcat attgcggaga aatcggaccg gctccggata accttatcgc
      301 cagagatttt aaggcggagc aacctaatca gaaatagctg acagatatca cggagttcca
      361 gttccctgca ggtaaagtct ggctgtcacc agtggtggac tgcttcgatg gaaaagttgt
      421 gagctggtct ctcagtacac gccctgatgc tgaactggtc aacactatgc tggatagcgc
      481 tgtcgaaacg ttaaatgctg gcaaacgacc ggtgatacac agtgacagag gtgggcatta
      541 tcgctggcca ggctggctgg aaagagtgaa tgcagcaggt cttattcgct caatgtcccg
      601 taaaggatgt tcacctgata atgctgcatg cgaaggcttt ttcggcagac tgaaaacgga
      661 aatgtattat gggcgtaaat ggtcgggcat cacgccagaa aagttcatgc agcaagtgga
      721 tgcttacatc agatggtata acgagcggcg tataaaatta tcgctgggtg ccgtcagccc
      781 caaaatgtac cgccaacaat gcgggctgga atgataaagc agtccaggaa atcgtccgca
      841 tccccccttc accatttgga ttgtccgctc cacccaacat gttgtttcct taaggttcct
      901 acaccacaaa ggaaactctc atgctgcgca gtggatggct gacgttgccg acaggcggga
      961 acttcgccag ttcaggcaga caccggaaca gcgcttcacg caggaacagg agcatctgca
     1021 gccgctgctg ggtacggact tcgatatccg tcatgtctcc tgggatggct atatcgaggt
     1081 tggcgggaac cgttacagtg tgccggaaag cctgtgtggt cagctggtat caatacggat
     1141 ctcgctggat gatgaactgc ggatctacag taatgagcaa caggtagcat cgcaccgtct
     1201 ctgttcggct gcatacggct ggcagaccgt gccggagcat cacgctccac tctggcagca
     1261 ggcttcggag cgcatggccg aacggctggg ggagatacag aaacgggtaa tcactgtctg
     1321 tgaccgggaa gcggatatct ggcattacct gtattataaa gtcagccacg gacagcgtgg
     1381 tgcgtgctgc acagaatcgc cggctggaag aggcacccgg caagctcttc gaactgccgg
     1441 aagtcctggc aaccgcagga agccacacgc tgaatgtgat gcaaaaaggc gggcgggcag
     1501 cccgtcaggc ccggatgttc atcagttaca gcgaagtcag cataaaaaat cccgacaaca
     1561 gcggccaggc gctcccgctc acgtatgtct gttgccggga gcaggcagag gacggtgcct
     1621 gctggcatct gctgacgtca ggaaaagcgg cgagtgccgc cgatgcacga cgtattgtca
     1681 gccattacga gcgacgctgg ctgacagagg aataccacaa ggcgtggaaa agtggtggta
     1741 catggaatcg ctgcgaatgc agacccggga taaccttgag cgcatggtgg ttatccaggc
     1801 gtttatagcg gtacgggtgc tgggcctgcg gcagggaggt gtcagtgaag aaacacaaaa
     1861 tgacagctgt gagaaaatac tgacgccgac agagtggaaa ctactgtggg ttaagctgga
     1921 aggaaagccg cttccggtgc aggcaccgac cctgaaatgg gcctggggcg atggcatgac
     1981 agcaaacgca caggtcgtcc cggttggggc gtcatgtggg atggctggtc agacttcagg
     2041 atatggttga gggttacctg gtgatgaagt ctcttgatca ggagctctga tcaagagaca
     2101 gcagct
//
LOCUS       DADLSA010000296         2094 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.301, whole genome shotgun sequence.
ACCESSION   DADLSA010000296 DADLSA010000000
VERSION     DADLSA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.301"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..>2094
                     /gene="ehaA"
                     /locus_tag="JN691_004270"
     CDS             <1..>2094
                     /gene="ehaA"
                     /locus_tag="JN691_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HAY7245488.1"
                     /translation="HVKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVN
                     NGGEVNAREHISLGYYDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAA
                     GIIDAEKIEFVWASTSDKKITLNHTDKDATISADIVSGSEGLGYINALNGTTYLTGDN
                     SAFSGKVKIEQNGALGITQNIGTAEINNCGKLHLKADDSMTFANKISGNGTISIDSGT
                     VELTGNNYAFSGYIDVASGAVAVISEDKNIGRAELDVDGKLQINANKDWVFDNDLEGR
                     GIVEINMGNHEFSFDEFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTV
                     GKGAHSISTLGFSGGTVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSV
                     SRDIDSALSLTEVDDGNSAIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQN
                     GQKAAVGTYDYRLTSGVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAK
                     ITGSGDLAFSSQKGQTVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETE
                     LDMNGHSQTVGTRNGSADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGD
                     NSNLTANVNIANSANVLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLT
                     NNGTLMTGMSGQQAG"
BASE COUNT          566 a          444 c          597 g          487 t
ORIGIN      
        1 tcatgttaag ggcaccagtc gtctgactta tacgcagggg aatgtaagcg gcgaaggtag
       61 tcaggtaaat gccgggacct tctttatggg cgtttacggc ggatatggcg gtaatcagtt
      121 cctgtcggtt aataacggtg gtgaagttaa tgctcgtgaa catattagcc tgggttatta
      181 cgaccaacgt tctgatacaa cattagtcgt atcagatgga ggtaaaatat ccgcaccgaa
      241 aattagttta agcaccaact ctgagttagc gttaggtgca caggaaggaa gtgctgcgaa
      301 ggcggcaggg attattgatg ccgaaaaaat tgagtttgtg tgggcttcga catccgataa
      361 gaaaatcacc ttaaaccaca cggataaaga cgcgactatt tccgcggata ttgtcagtgg
      421 cagcgagggc ctgggctata tcaatgcgct caatggcacg acttacttaa ccggtgataa
      481 ctctgccttt agtggtaaag tcaaaattga gcaaaatggc gctttaggga tcacccaaaa
      541 tataggtaca gcagagatca acaactgcgg gaaattacac ctgaaggctg acgatagcat
      601 gacctttgcc aataagattt ccggcaacgg tacaataagt atcgacagtg ggacggtgga
      661 gttgaccggc aataactatg ccttcagcgg atatattgat gttgcttctg gtgctgtcgc
      721 ggttatttct gaagataaga acatcggccg tgcagagctg gatgttgatg gcaaattgca
      781 aattaatgcc aacaaagatt gggtatttga taacgatctt gaaggtagag gcattgttga
      841 aataaacatg gggaatcacg aattctcctt cgatgagttt gcttatacag actggttcca
      901 gggttcactg gcgttccaga acacgacatt taatctggaa aagaatgctg agtttctgca
      961 gaaaggcggg atcactgcgg gtcagggaag cctggtaaca gtgggtaagg gcgctcactc
     1021 cattagcact ttgggattct ccggcggaac cgttgatttt ggtgccctga cagcaggtgc
     1081 acagatgaca gaagggacgg tcaacgttag taaaacgctg gatttgcgcg gcgagggtgt
     1141 gattcaggtt tctgacagtg acgttgtccg ctcagtatct cgtgatattg actctgcgtt
     1201 atcgctcact gaagtcgatg atggtaacag cgccattaag ctggttgatg cgcaaggtgc
     1261 ggaagttctg ggcgatgcgg gcaatctgca attgcaggat aaaaatgggc aaatcctctc
     1321 cagcagtgcc caacgtgata ttcagcagaa tgggcaaaaa gcggccgtcg gcacttacga
     1381 ctatcgtctg acgagtgggg taaacaatga cggtctgtat atcggttacg gcctgaccca
     1441 gcttgattta cacgctaccg acagcgatgc tctggtgctg agctctaacg gtaaaagcga
     1501 gaatgccgcc gatctcagcg caaagattac cggcagtggt gacctggcat tcagcagcca
     1561 gaagggtcag accgtatcgc tttctaacaa agacaacgac tataccggtg ttaccgatct
     1621 gcgcagtggg acgcttttgt tgaataacga taacgtgttg ggtaataccc atgaactgcg
     1681 tctggcggca gagactgaac tggatatgaa tggtcacagc cagaccgtgg gcacgcgcaa
     1741 tggcagcgcc gattcactgc tgagcttaaa tggcggcagt ctgacggtta ccaacggggg
     1801 cacttcaacc ggttcgttaa cggggagcgg agagctgaat attcagggcg gcacgctgga
     1861 catcgcgggc gataacagca acctgacggc gaatgtgaac attgctaatt cggctaatgt
     1921 cctggtaagt catgcgcagg gattgggtag cgcaaacgtt gagaacaacg gtaccctggc
     1981 gttgaataat agcgctgaaa aaagagcggc tgcgtctgtg aattacaccc tgggcggcaa
     2041 tctgaccaac aacggtacgc tgatgaccgg aatgtcagga cagcaagctg gcaa
//
LOCUS       DADLSA010000297         2071 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.302, whole genome shotgun sequence.
ACCESSION   DADLSA010000297 DADLSA010000000
VERSION     DADLSA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.302"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            7..2071
                     /locus_tag="JN691_004271"
                     /pseudo
     CDS             7..2071
                     /locus_tag="JN691_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          777 a          363 c          380 g          551 t
ORIGIN      
        1 tacatcatgt acaaatcatt atttactatt atcattctac tgtatagcgc cagttcatat
       61 gcaaaaagcg aatcccctcc cgcaagcact ataactttca ttgcacaaat cgatcatgtt
      121 gagctaaata aaacagcatt atctcatgat atgaaactcg ttcttgccga tcgtttttat
      181 ttcaaaacca gtaatccttg cgaaacaaat tcaatttctg ctcgaccaga atcaatggat
      241 attactgaca aagcctatgc cgacaaatta aaaaaattag aaactccttt tcttgatgat
      301 cgctatttct ttatgaccat caatcagtgt gacggtaatg gcactcccat gctgacagac
      361 ataaaagtat gtacaacaga attatgtggt gctgaatata caaagaaaat gtctactgta
      421 tggctcgatg aaactttaaa accaactgct aaacgtctgg caatttattt tattcaatct
      481 cctttaccct atgacgagga gaaaaaacaa tggaaagtca ccggatggta tatctcatca
      541 actgataatc agtcttcaac taaacagatt gcatttgaag gatatacaga cacagataac
      601 ttctccgatt ctaaatttat ttcttcattt aaatcctggt ttgaaagtgg aaagataaaa
      661 aacattgcta actataatcc aaaaggagaa agagatggaa aatacgaaag ttattacaat
      721 agcaatggta acgtctatga atctctaagt ttttctaatg gtcttatcaa cggtgagttt
      781 attacatatt atgaaaatgg ggccattgaa agtaaacgtc attttaataa cgacaaaatt
      841 aaagatggtg aatgccctca tttttatgaa tgatggaaaa atcaaacagc aacatagcta
      901 tctgaatagt aaactcgatg gtcctgcata tgaatattat cctgatggca aactcatgca
      961 ggaaaatttc tatcagtaaa gtgtgttgat tggaaacgat accagttact accaatccgg
     1021 aaaaataagc tctatacaca atagaaattc ccgtggtcag tatgatggag ctaacgaacg
     1081 ttatagtgaa gagggtaagt tactgtctaa atccgtctat aaagacggaa aacaaatttc
     1141 tgttcaaacc tggtatgaaa atgtgcagaa agaagaggag aaacattttg atgagcaagg
     1201 ccaattaaat ggtctggtta agcaatggta taaaaatggt aatcttgcaa aatcacaaaa
     1261 ctataaacat gacatactcg atggtaacag tgaagagtgg tacgaaaatg gcatccctga
     1321 aagtttgtac ccttataaga atggaaaaac cgacggcatt gccaaatcat ggaataagta
     1381 tggtaaatta acctattcaa tcgaatacaa aaatggcgta gagaatgggg catatcgtaa
     1441 ctggagcaaa aacacaggta aactaaccaa agaaaccctg tacgtcaatg gtatcagaca
     1501 gggagtggaa aaagagttta acgatcgcac aggtaaacta ttaacctcca cgcaatatgt
     1561 taataataaa agacatggta cagaggaaac ttatgatcaa aacggaataa aatatattac
     1621 atgctatcaa aatgatcaga aattatcgtc tttagacaat ccaacacaaa taaaagataa
     1681 tgcaacaaca ggcgattcca gtgctcaatt tgcattaggg aaatacgaat tcatctgtgc
     1741 aaatatagat gaagggatta aatggttaac caaatctgca gagcaaaaaa acactgatgc
     1801 gatatacttt ttagctacag cctacaaagg caatggtata cctgcaaaca atgaaaaata
     1861 cctggcatat ctgcaacaag ctgcaacact gggcagtagt aatgcccagg cggaaattgg
     1921 ctatttatat ctcatcggaa aagaactacc acagaatctt cctgatgctg gtgtctggtt
     1981 caaaaaagca gctgcacaag gtaattttgc cgcacatttc tatctgggaa ggatgtatca
     2041 gaatggtgac ggcgttgaga gaaatatgga a
//
LOCUS       DADLSA010000298         2041 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.303, whole genome shotgun sequence.
ACCESSION   DADLSA010000298 DADLSA010000000
VERSION     DADLSA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.303"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(218..382)
                     /locus_tag="JN691_004272"
     CDS             complement(218..382)
                     /locus_tag="JN691_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245489.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1284..1568
                     /locus_tag="JN691_004273"
     CDS             1284..1568
                     /locus_tag="JN691_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY7245490.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1556..2041
                     /locus_tag="JN691_004274"
     CDS             1556..2041
                     /locus_tag="JN691_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7245491.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          526 a          425 c          585 g          505 t
ORIGIN      
        1 aaaatgatgg acattatttt tgtggaaccg gaggtaacag accagacggt ttaaatgagc
       61 cggttacttt aaacgggtga tggacagcac ctcaaaagtt cagatggatt atatttgtct
      121 gcaatacccc gggtattttc gatttgcgaa atggatggaa cgacttgctc aggggatagc
      181 tgatggagtt actgaagtac cgaaggacca ctaaaaatca caggccggga atttcggcgt
      241 ttccggcgta ggtgtgccac gaatgcatcc agtggcgtga tgttggatgc aatgctgtat
      301 gagagatgag gggcggcgtc cagacgcagc cttcaaaccg ccttgagctg ctgccgttaa
      361 gagcggtggt ggtgagcgtc aaggttaaaa ggcgcagaac atgtgtcagg taggtgttgc
      421 ggagatgaac gaaagtgaac tgtcgatgaa gtgtcgaaag gaacctctag gcgatgtcga
      481 aaatgtgcta tctacgcagc gcattacgag tccagcagat atctggtact ggctgggcgg
      541 catccggcat ataggcagca tgaacgcaac acaggctctc gtacggaacg tgggaacctg
      601 tcgtctcgat gctaagggag cggcgcggag cagaggcact gcaagcgcct gagtaccgat
      661 gcgagacaca ggggcagatg gcttcgtagt agtgaggaag tgcctgtaat gggcatggag
      721 cgaagggggt cagtcgttgg ggctggttta tcggtcaacc ggatagccgg gaggagccca
      781 tgagtcagcc aaagccgttc gaggtctcta agtatgctgt ttgaaaagca taccagcggg
      841 tcaaagctaa tcgaggtgcg gcaggtgtcg atgggcagtc agtcgaggcg tttgaggtta
      901 ccaatcgaag ctatacccgg taatggcgca ggttgacctg cacttggcca agtggattgc
      961 ccgcaaacac aagcgagcgc gaggtagtct ggtgcgtgca ttcgagtggt tactgcgaat
     1021 tcgtcacgac tgcccgacgc tgtttgcaca ctggtgctta gcttacgata cttgaatggt
     1081 tcaacggcaa gagccgtatg acgtgagagt gtcacgtacg gttccgtggg agcgtagggg
     1141 ggcggtgccc ctgcgccacc cgatatcccc tttgagtgcg gttacgtcca cagaggtaaa
     1201 tgccgctgac gcggcatctg taatctgtta cgttgtgtat atcatttgct atatattgtc
     1261 aatatgctat tgttgtaaag caaatgatat acggaggctt tatgaaatca ggtgttcagc
     1321 tcaatctcag agcaagagaa tcacagcgga tactcattga tgctgctgca gaaattcttc
     1381 ataagtcgcg tacagatttc attctggaaa tggcctgtaa agctgctgaa gatgttattc
     1441 tcgaccggcg ggtgtttaat tttaatgaca ggcaatatga agagttcatt gaaatgcttg
     1501 atgctccggt tgcagatgat cctgccatcg aaaaattgct ggcaaggaaa cctcaatggg
     1561 atgtgtaact gcaccagaac ctttatcctc atttcatcag gtcgctgaat ttgtcagcgg
     1621 tgaagctgta cttgatgact ggttaaagca gaaggggcta aaaaatcagg caatcggagc
     1681 cacccggact tttgttgtat gcagaaaagg tacgcaacaa atagtcggtt tttactcact
     1741 ggccactggt agtgtcaatc atacagaagc tacaggtaac cttcggcgca atatgccaga
     1801 ccccattcct gtaattatac ttgcacgcct ggctgtagat gtgtcatttc gaggaaaagg
     1861 gcttggcgcg gatttacttc atgatgcagt tcgtcgttgt tacagggttg ccgaaaatat
     1921 cggtgtgcgt gcaattatgg ttcatgcgct tacggaaaac gcaaaacagt tttatattca
     1981 tcatggtttt aagccatcaa aaactcaggt gcaaacattg tttcttaaac tccctcaata
     2041 g
//
LOCUS       DADLSA010000299         2026 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.304, whole genome shotgun sequence.
ACCESSION   DADLSA010000299 DADLSA010000000
VERSION     DADLSA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.304"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..663)
                     /locus_tag="JN691_004275"
     CDS             complement(<1..663)
                     /locus_tag="JN691_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAY7245492.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSG"
     gene            complement(691..2022)
                     /locus_tag="JN691_004276"
     CDS             complement(691..2022)
                     /locus_tag="JN691_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY7245493.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
BASE COUNT          554 a          596 c          453 g          423 t
ORIGIN      
        1 accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga gaataaagcg
       61 gatcgccgca aacgggactt cattgacgaa cttcaggatc tcagccagcg tcgttctgga
      121 aagcaccgtc agcggagact cttgctcgcc ctctgccaca cacgcctgct gggtaaacac
      181 cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga tggtgacaca
      241 cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat tgcaaggttc
      301 ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg caattgcctg
      361 cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta acgcccccag
      421 cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc ccagaccgtt
      481 cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt ccagttctgc
      541 cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca gcgccggttt
      601 tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag tcgaatcaaa
      661 catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca gagaaacaca
      721 aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt tgtgcaatgc
      781 cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga aaatcgggct
      841 acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc aggccagcaa
      901 aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct cggcaggcat
      961 cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc ctaaatagac
     1021 gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca ctttcaccca
     1081 cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa tattctgctc
     1141 gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg cgtagaaaaa
     1201 gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt tatgccgcgc
     1261 cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta acgtctggat
     1321 aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca ccagcagccc
     1381 cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg acacgcccag
     1441 cgcagcgact accagcagct tggtcagcac catgccggtc gaaattttga ataacaattt
     1501 ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat aaaagggact
     1561 gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac tatcgttggt
     1621 gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga gcgcacctaa
     1681 caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat ctttacattc
     1741 tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg ccgggtaacc
     1801 aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca ccgggagaaa
     1861 aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg tgtcagtact
     1921 gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac ccgccggggt
     1981 cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatt
//
LOCUS       DADLSA010000300         2020 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.305, whole genome shotgun sequence.
ACCESSION   DADLSA010000300 DADLSA010000000
VERSION     DADLSA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.305"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            278..1378
                     /gene="ompC"
                     /locus_tag="JN691_004277"
     CDS             278..1378
                     /gene="ompC"
                     /locus_tag="JN691_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY7245494.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1542..>2020
                     /locus_tag="JN691_004278"
     CDS             1542..>2020
                     /locus_tag="JN691_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY7245495.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSL"
BASE COUNT          629 a          402 c          391 g          598 t
ORIGIN      
        1 tattagcagt aattttgtgg ggatccctca ggtctacggc ccggcagtta atcagataaa
       61 ttccgacaat attaaaaaat aaatttcaaa taccagtaat tcaccttata tctcatttcg
      121 acttcgatca catatcgata aataatatta tttcatctat ttttctcatt gtttttaatc
      181 accattctat tttaaaaaca gtatgtcatt tataaatgac gccgtttaaa attcgtttag
      241 aaaatacgtt taacgataat taaaaggatt ctgttaaatg aaattaaaaa tagttgcggt
      301 ggttgtaact ggtttgttag ctgcgaacgt agcacacgct gccgaagtct ataacaagga
      361 tggtaataaa ctcgaccttt atggcaaggt taccgctcta cgttatttta ctgatgataa
      421 gcgtgacgat ggtgataaaa cttatgcccg tctcggcttt aaaggagaaa cgcaaatcaa
      481 tgatcaaatg attggttttg gtcactggga atatgatttt aaaggctata acgatgaagc
      541 caacggctcg cgcggcaaca agacccgtct ggcctatgct ggtttaaaaa ttagtgaatt
      601 tggctctctg gactatggtc gtaactacgg tgtcggctac gacattggtt catggaccga
      661 tatgttgcca gaatttggtg gcgatacctg gagtcagaaa gatgtcttca tgacataccg
      721 taccaccggt ctagcaacct atcgcaacta cgatttcttt ggcttaattg aagggctgaa
      781 ctttgccgcg caatatcaag gcaaaaatga acgtactgac aacagtcatc tttatggtgc
      841 tgactacacg cgtgccaacg gtgacggttt cggtatctcc tcaacttatg tttatgatgg
      901 ctttggtatc ggtgcggtat ataccaaatc cgatcggaca aatgcgcagg aaagagccgc
      961 tgctaatcct ctcaatgcct ccggtaagaa tgcagaacta tgggccacag gtataaagta
     1021 tgatgccaac aacatctact ttgcagctaa ttacgctgaa acattaaaca tgaccaccta
     1081 tggggatggt tatatctcta acaaagcaca aagttttgaa gtggtggcgc aatatcaatt
     1141 cgacttcggc ttgcgcccat cactcgctta cctgaaatcg aaaggcagag atctgggccg
     1201 ctacggcgac caggacatga ttgagtatat cgacgttggt gcgacgtatt tcttcaacaa
     1261 aaatatgtcg acctatgttg attataaaat caacctgatt gatgaaagcg actttacccg
     1321 tgccgtagat attcgcaccg ataacatcgt cgctacgggc attacctatc agttctaacg
     1381 tcctctcctg accagccctt ctctgggctg gtttttgatt taagtgctgc tgtatgactc
     1441 cttaagtaaa aatgagcaat ttttgatatt tctggtcatt cacctcaatt aatattattc
     1501 catatcctcc ggggacatct tactataata tttattgatg agtgattatg accaatatta
     1561 atacagcttg tgtaaaaaat aatgccagtt atcaacttaa taacgcatta cctaacaaag
     1621 aaaccatctc cagcaatttt tgtgaacgac tagaacaatg gggtaataaa tcggttaata
     1681 atggtgaaga aagagcaatt gccgtggagc gaattaaaga agcttacaat tcgaacatgg
     1741 catctcttga tttgtcatat cttgatttga gtgaattacc gcctatcccc tcgacagtta
     1801 atacgttaaa tctggaaaac aattgtctaa cttgtcttga cttagctgac aatgccagtc
     1861 tcgttaatat caacctcagc gttaataaaa ttaacacgat aacctttcca aaagaaccaa
     1921 aactggaaaa catttatatt gatcataaca atctggaaag tttagattta aaaaatcagc
     1981 attcattggt taatctggaa gcgcaaaata atagcctaaa
//
LOCUS       DADLSA010000301         2004 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.306, whole genome shotgun sequence.
ACCESSION   DADLSA010000301 DADLSA010000000
VERSION     DADLSA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.306"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(131..1585)
                     /gene="ospC2"
                     /locus_tag="JN691_004279"
     CDS             complement(131..1585)
                     /gene="ospC2"
                     /locus_tag="JN691_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HAY7245496.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          636 a          345 c          317 g          706 t
ORIGIN      
        1 caggattccc ggggcgttca ccacatgcaa tgtgttgttg caggggaacg gtctgcacca
       61 tcccctatat cgatcaatat aacaaaaatg acaacgtcag attcagggca caacctaaat
      121 atattttagg tcacctccca aatcgtttgc ctgataccgc tccatttttt aataaaaaat
      181 caattgtttt tgaatctttg cttttcattg cattgtcaag catagtgtcc ccactatttg
      241 ctttttgaaa tcttttgttt acatcaacca atccattatt aataaaatat tctagcactt
      301 tatagttggc actatcttca ctaagtaaat actcaacatc atacagctca caatacatat
      361 tgttgttcat tttctccatc tctgcgacat cttgttttgt aaaattaaat ttagatagta
      421 aataaagtgc catatcagat tttgcattgc ctagagaata agcaagagct tgatgagcca
      481 tttttttatc agtcacctta ttgttaattt cttcataatt ggatgcttta atggcatcct
      541 ccaaagatat atctctgagt cttatctttt tgaagttatc tgtgcttagc attcttggta
      601 tatgatactc aagctgaaac acaaagttta taattctatc cagactaaca ggatcaatgt
      661 ttttattata acaaaactct cggaatcttt gatctttact ctttcttata aaatcaataa
      721 gatgcaaccc aattccaagc ctcatatctt tagtattaaa tattggcaca tcagtttttc
      781 cctgattacc atgcacatac ctgctaactt catccacaac ctctttaaag ttatctaaga
      841 aaaatgattg gtgctcatgg taaaaaacag gtggaatagc attatcaaag tggtcggtta
      901 atgtcatata tccatatgga gagtcatgat caatgatata cgcattagca ccaaaatcca
      961 cagtatgatg tgttttattt agtgggagtg tttcttgatg attggaaatt tcaaccccaa
     1021 aaaaaacaaa atcgtggtta gctatttctt caatatcctc agagaatgtg tttttatatg
     1081 tttgtatatt ctcagactta attttatcaa aggacttaat attaagagta ttatttgaca
     1141 ggttagcgtt actttgatgt ctgaagttaa aatcagaatt aataataata gagagcaagt
     1201 gacgttcatc cgaagaaaga ggatgagttt tttgtattat ttcagaaaga tagtgtctgg
     1261 acaccttatc gaggtgctca agtgaccgaa atgatgaccg tgctccagat ggcgcttcaa
     1321 tagaaagatt taatgatttg aaactggtcc cttgagagaa cattttacta taactttgcg
     1381 ctgctataga cttccttaaa aagcttttca cagtatcagc acagtgatct aaatgttttt
     1441 cacttatgcc agaagaggat gagtttgtta cgcatgatat gtttttttgt aatgcttttg
     1501 tatctttgtt cggatttatt tttccatcaa caagatcaat attgttctga acattaatat
     1561 gatttactgc ttcaggtatt ttcataaaac atcccccatt atgtgcgttt ttatctgcag
     1621 ttatttagaa taatattatc taacttaata ttttcttgta tgtattataa cgaacatatt
     1681 ttaatatatt gtacgatttt cactctgaaa tcccctgagt ttaatcacac ttcctttact
     1741 gaaaggaatt attgcaacag tattatttgt ttttggatat atatcttgca ccttgttatg
     1801 tagttaatgc tcagaatatg atagataaaa tagagacgca gaacagggca tgctcataac
     1861 atgatgataa cataaaaaat tagtaacaat aaatgaaaga atcatgggcg ctcaacaaaa
     1921 agagagctaa atagctgcgc ggaatagtag gctgtgtccc ttaatcatct gagttatagt
     1981 aactgtctgt ttacacaaca tatt
//
LOCUS       DADLSA010000302         1998 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.307, whole genome shotgun sequence.
ACCESSION   DADLSA010000302 DADLSA010000000
VERSION     DADLSA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.307"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..149
                     /locus_tag="JN691_004280"
     CDS             <1..149
                     /locus_tag="JN691_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245497.1"
                     /translation="YREIDAIARKYVSLKYDGSIASDFSTMKCIDFIHGNELDELISR
                     SVKK"
     gene            263..748
                     /locus_tag="JN691_004281"
     CDS             263..748
                     /locus_tag="JN691_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HAY7245498.1"
                     /translation="MSYDIFLEIDGIDGESMDDKHKNEIEVLSWRWNIHQESTMHAGS
                     GLGSGKVSVTNLSFEHYIERASPNLFKYCASGKHIPKAVLTMRKAGGNPLEYLKYSFT
                     DLIVAMVSPSGSQGGEIASRESVELSFSTVKQEYVVQNQQGGSGGTITAGYDFKANKE
                     I"
     gene            816..1349
                     /gene="tssJ"
                     /locus_tag="JN691_004282"
     CDS             816..1349
                     /gene="tssJ"
                     /locus_tag="JN691_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HAY7245499.1"
                     /translation="MNKWRNPGGWLCAVALSFVCLLSGCSIDSDDVPDLMSQRLDLVI
                     KASDKVNPDNQKKAAPIEIRVYELKNDAAFTAADWWSLQDNDKAVLADDLLHRDSFIL
                     RPGEEKKLRHALNKETTTLGILAGYRNLGKSVWRATYKIPEPPEKSWYSRFMPKKKVE
                     LDAGIEQSAIVIKERDK"
     gene            1353..>1998
                     /locus_tag="JN691_004283"
     CDS             1353..>1998
                     /locus_tag="JN691_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HAY7245500.1"
                     /translation="MSWNNRVVWSEGQFLLPQMFQQQERYLEHVIHYRSLPLSPFFWG
                     FNEYGIGSEALNIGKLVLTKASGVFADGTPFNAPGHTPLPPPLTILPEHLNQLIYLAV
                     PIRTPNSEETSFDNNPESLARFSVHENEIRDANSLGRGAQLLQLSHLRLRLLPEKAVT
                     DAWIGLPLTRITALHPDGRVDIDKELIPPVANYQASTLICTWLSWINDLIKMRAD"
BASE COUNT          560 a          445 c          501 g          492 t
ORIGIN      
        1 tttatcgtga aatagatgcg atagcgagaa aatatgtatc attaaaatat gatggttcta
       61 tagcatcaga cttcagcact atgaaatgta tagatttcat tcatggaaat gaattggatg
      121 agttgatttc caggagtgtt aagaaatgaa atatattcaa tcctttgttc tgccgattcc
      181 atatgtggaa gagtgttgta aagtaatggt ttatttgtaa gcggataaac caaaattaat
      241 ttaaataaaa aaggtatgaa atatgtctta tgatattttt ctggaaattg acggcattga
      301 cggcgagtca atggatgaca aacataaaaa tgaaattgag gtgttgagct ggcgctggaa
      361 tatccaccag gagtccacca tgcacgcggg tagcggtctg ggatccggta aggtatccgt
      421 aactaatctt tcatttgaac actatatcga gcgcgccagc ccgaacctgt tcaaatactg
      481 cgcctccggc aaacacattc cgaaagccgt gctgaccatg cgcaaagcag gcggcaaccc
      541 actggaatac ctcaaataca gttttactga tttgatcgta gcaatggttt cgcccagcgg
      601 gagtcagggg ggagaaatag cttctcgtga gtcagtcgaa ctctctttca gcaccgtgaa
      661 gcaggaatac gtggtgcaga accagcaagg tggcagcggc ggcaccatca ccgcaggcta
      721 cgacttcaag gccaataaag aaatttaagc agtacttttc cggcccggca aatagccagg
      781 ccggttttta ttatttgatt ttaaaggaaa tcacagtgaa taaatggcgt aaccccggtg
      841 ggtggttatg tgcggtagct ttgtcttttg tctgcctgct ttccgggtgc agcattgaca
      901 gcgacgacgt gccggacctg atgtcacaaa gactcgatct ggtgattaag gcttccgata
      961 aggtgaatcc tgacaatcag aaaaaagccg cgcccataga gatacgcgtt tatgaactga
     1021 aaaatgacgc agctttcacg gcggcggact ggtggtccct tcaggacaat gacaaggctg
     1081 ttcttgctga tgatttactt caccgcgaca gctttatcct gcgtccgggc gaggagaaaa
     1141 aattgcgcca cgccctcaat aaggaaacta ccacgctggg gatcctcgcc ggataccgca
     1201 atctgggaaa atcggtctgg cgggctacat ataaaattcc tgagccaccg gaaaaaagtt
     1261 ggtacagccg ctttatgccg aagaaaaaag tcgaactgga tgctggcata gagcaaagcg
     1321 ccatagtgat taaagaacgg gataagtaaa ttatgagttg gaataaccgg gtggtctgga
     1381 gtgaaggaca atttttgctg ccacagatgt ttcagcagca ggaacgctat ctggaacatg
     1441 tcattcatta tcgcagtttg cctctttcac cttttttctg gggcttcaat gagtatggaa
     1501 ttggtagtga ggcgctgaac atcggaaaac tggtacttac aaaggcttcc ggggtatttg
     1561 ctgacggtac tccttttaat gcgccgggtc acacaccact cccaccgccg ctgaccatcc
     1621 ttccagaaca tcttaaccag ttgatttatc tggctgtgcc aatacgtacg ccgaacagtg
     1681 aagaaaccag cttcgacaat aaccctgaat cactggcacg cttttcggtg catgaaaatg
     1741 aaatccgtga tgccaattca ctggggcgcg gcgcgcaact gctgcaactt agccacctac
     1801 gcttgcgact attaccggaa aaggcggtga cagacgcgtg gatcggcctg ccgctgacgc
     1861 gcatcacggc gctgcatccc gatggtcggg tcgatataga caaagaattg atccctccag
     1921 tggcgaacta ccaggccagt acgctgatat gtacctggct atcctggatt aacgatctga
     1981 taaaaatgcg tgcagatt
//
LOCUS       DADLSA010000303         1978 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.308, whole genome shotgun sequence.
ACCESSION   DADLSA010000303 DADLSA010000000
VERSION     DADLSA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.308"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..881
                     /locus_tag="JN691_004284"
     CDS             <1..881
                     /locus_tag="JN691_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7245501.1"
                     /translation="GPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWL
                     TLLSVNLYFNGLRYDIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLT
                     VMVIFMCHIFSALRVTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDID
                     HFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKAL
                     AERIRENVERLTGDNPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETG
                     RNKVVVRDVVNFCESP"
     gene            979..>1978
                     /locus_tag="JN691_004285"
     CDS             979..>1978
                     /locus_tag="JN691_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7245502.1"
                     /translation="MLIIDQSAITRFYWQLYYNFLGCITLMRGETCMVFNKKMFVLII
                     IPGILGVLLSFAMSIFQMNRDTTITAGILLKQLDNVTQIVQHTTKLTSILVMKPCKDI
                     LEQLIANGALTPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKNKESRIVSI
                     SGTSFVPGKTAIVFLMPIGNDMTAFSIVESRYIYDLMDVLDDENDDSFSLRFTEGPAI
                     ISGVNNNDRLYMLKRDFNSAISQARLTVTTPMISLYPYIISNVLYILPLSIILSFILY
                     FLWQHWMSRKMSLAEEIKKGMSSGEFSVHYQPVCDTTTKACLGVEALMRWQREDGKN"
BASE COUNT          593 a          345 c          428 g          612 t
ORIGIN      
        1 gggggccgat agttgctcac atactttcta gtcattatga gagctacaat ttacacattg
       61 ccgagttaac caatgaaaat ggtcaggtgg tttggaaagc ctcatatgtt accataatga
      121 ttttcatgtg gttaaccttg ctttcggtta atctatactt taatggttta cgctatgata
      181 tctggaatgg agtgacggtg attgcttttt gtgccgtact gtacaatatc tctctgttat
      241 ttatgagcag gtatagcgtg tccacttggt atattagtcg cactattgaa gtagtcagta
      301 agttaaccgt tatggtaata tttatgtgcc atatattcag tgcgctacga gtaacaaaga
      361 acattgcaca tcgcgatccc ttaaccaata tatttaacag aaattatttt tttaatgaac
      421 tgacagttca atcagcatca gccaaaaaaa cgccttattg tgtcatgatt atggatatcg
      481 accacttcaa aaaagtcaac gacacctggg ggcatccggt tggcgatcag gtgataaaaa
      541 cagtggtgaa tatcattggg aaaagcatac gaccagatga ccttttagcg cgcgtcggcg
      601 gcgaagagtt tggcgtcttg ctgacggaca tcgatactga acgtgcgaaa gctttagcgg
      661 aaaggattcg ggaaaatgtt gagcgtttaa ctggcgataa tcctgaatac gctataccac
      721 aaaaagtgac gattagtatt ggcgctgttg tgactcagga aaatgcgtta aacccgaatg
      781 agatctatcg actggctgat aacgcactat acgaggcgaa agagaccggg cgtaataagg
      841 tggttgtgag ggatgtggtg aatttttgtg agtcaccata aagcggcatt ttagcgggtg
      901 attttggtaa gcgctaaata gctattcgca gacttgcgta acaattttca gaaatatcat
      961 agtactatat gatcttaaat gcttatcatt gatcaatctg ctatcacccg cttttactgg
     1021 caattgtact ataatttctt aggttgcatc accctgatgc gtggggaaac atgtatggta
     1081 ttcaacaaaa aaatgtttgt actcattata ataccaggca ttctgggggt gttactatcg
     1141 tttgctatgt caatatttca aatgaataga gatacgacaa ttaccgccgg tattctgtta
     1201 aaacagcttg ataatgttac gcaaattgtc cagcatacga caaagcttac ctctattctg
     1261 gtgatgaagc cctgcaaaga cattcttgaa cagttgattg ctaacggagc attgactccc
     1321 tatgttcgaa caacgggtct tatagaaaat aatttccaaa tatgttcttc agttagcggt
     1381 ttcaaaaaaa tgaatgttaa cgatgtttac ggtacgagct ttcacaataa aaataaagaa
     1441 tcacgtatag tatcaattag cggtacaagt tttgtgccag gtaaaacagc aatagtgttc
     1501 cttatgccaa tagggaatga tatgacagct tttagtattg tagagtcacg atatatctat
     1561 gatctaatgg atgttcttga tgatgagaat gatgattcat tttctcttcg ttttactgaa
     1621 gggcctgcaa ttattagcgg tgtaaataat aatgacaggt tatatatgtt aaaaagagat
     1681 tttaattctg cgatcagtca ggctcgtctt acagttacta cgccgatgat atcgttatat
     1741 ccctatatca ttagcaatgt gctttatatt ttgccgttat ctattattct ctcttttatt
     1801 ctctatttct tatggcagca ctggatgtca cggaaaatgt cgctggcaga agaaattaaa
     1861 aaaggaatgt catccggaga attttctgtg cattatcaac cagtgtgcga cacgacaacc
     1921 aaagcgtgtc tgggggttga agcgttaatg cgctggcaac gcgaagatgg taaaaata
//
LOCUS       DADLSA010000304         1929 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.309, whole genome shotgun sequence.
ACCESSION   DADLSA010000304 DADLSA010000000
VERSION     DADLSA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.309"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            463..1512
                     /locus_tag="JN691_004286"
     CDS             463..1512
                     /locus_tag="JN691_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY7245503.1"
                     /translation="MYNNKIDSNRKTRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSIFDYIKSKFYRLMIPYFTFAFTILIINTINSGETI
                     DYIYSHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFKEIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKFGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
BASE COUNT          626 a          326 c          356 g          621 t
ORIGIN      
        1 tctgcttttg ggaggcatgg gtcactcagg ccaaatgaca tccgacggtt tcacgatgaa
       61 ctacggcaaa agaatatcag agccctcatt cctccccgaa agggagcggg ctctggtctg
      121 gtgagtatgc agatcgtaaa ggctatggcc atggtgcgag agtggcagct atgccagaaa
      181 gagtgtgtat tgcctgagac tctaacctga tgcagagacg attgtcttga acctgattta
      241 ttcaacaaag ccgctggcag gatgttacga aagttatcgc ggccgcaggc tgatttgata
      301 tcgatactat cggtggtact gcggaagtaa atgcctatat agtgcgactt tgaaaaataa
      361 taaattaata ttgaactcgt tgattaataa tcataaactt tcaggcattc atatttgcaa
      421 tgagtgtttg aaaatcacac aaaactggag agcctacttt aaatgtataa taacaaaatc
      481 gacagcaaca gaaaaacaag gcatgattgg gttgatgctt taaaatttct cggtattttt
      541 gctatatatt taggtcacct tggtttgggg gctggcaaat tgtatccttt tgtttttagt
      601 tatcacgtcc cactattctt tttcgctgct ggttttttta ctatcaaaaa aaatgactta
      661 tctattttcg attatatcaa gtcaaagttt tacagattaa tgattccata tttcactttt
      721 gcatttacta tattaataat aaacacaatt aactcaggtg agacgattga ttatatatat
      781 agccacattt atgatataat atatggtgtt agaaacaacc aatttgtagg aacaatatgg
      841 tttataaact gcctttttgt tataatagca attgatgcaa tctttaaaga aatagtcaag
      901 aacaatatag tgattttaat catatcacta ttatcgttca tgttgtcaca aacagtgctc
      961 aatcataacc cattactaga tcctcaatgg ttttggaaca tagactccgc tatggcatac
     1021 tggtggttat tgcctctcgg tagatgtatg tttttagaat taacacgtga caggtttttt
     1081 ggaaaatcta aatttggttt cattgtcttc tcaattaccg cgataatgag tgcgtatcaa
     1141 ctccttaacc agaaaccttt gctttttaag attatctcaa tattcaatgc agacataata
     1201 tcaagtagtt atattcaagc tattaatact attatcacca cggtaggttt gataatattt
     1261 aatattttta tagcaaaaat aatttgtggg aatgacttta ttgtaagagc tggaagaaat
     1321 actcttaaca tatgtggaat ggagtatatc accaaattat ttatccctat ggctttagca
     1381 atcataggct tcagcgtgac cattccaaat cctatctgcg caatcatata tacctgtatt
     1441 tgtgtatatg tatcagataa aattggccat tggctatcga ggacagtcgg tgggccattt
     1501 ttgataaaat gataaaaagg cgcaattaat tgcgcctttt tatagatata tagcgcatat
     1561 ttatgctcag attatactgt taaaattaat gcgcaaatgt tacggttttc tttcttggca
     1621 actgaaggga atgttcgaag tgcaaacata aaattacccc ttcactgaac agatattcag
     1681 tcggccaaca catgagaaag aaatacacca gatgaattaa aatatcatat actcattaat
     1741 caaagaaata aaagcataaa cagtaggtgg tgacgctgat aaactggcta taaacaacca
     1801 ggaaggtgat gagctatcgc aatataattt attggtgaat tatttgaagc cgattgataa
     1861 caccagtgct caaaagactt cccccggtaa ccataagtag ctaatatcta gtacagtttc
     1921 gatatctat
//
LOCUS       DADLSA010000305         1927 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.310, whole genome shotgun sequence.
ACCESSION   DADLSA010000305 DADLSA010000000
VERSION     DADLSA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.310"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..1208)
                     /gene="pdeC"
                     /locus_tag="JN691_004287"
     CDS             complement(<1..1208)
                     /gene="pdeC"
                     /locus_tag="JN691_004287"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAY7245504.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVPVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDV"
     gene            1636..1917
                     /locus_tag="JN691_004288"
     CDS             1636..1917
                     /locus_tag="JN691_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245505.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          553 a          424 c          427 g          523 t
ORIGIN      
        1 tcgacatcaa gaaatgcatg ctcagtcact tcaaatttaa tttgctgtgg acgcaccgcg
       61 tattgctctg ttttctgatt gattcgcgct atcaaccgtg acgtatgaaa atcggaggcc
      121 gacaggttaa tagaaacata gcgatcggca tgtgttgcca ggtacgcgcc cagatcgcgg
      181 aagacattat caataacata atcagttatc tgttctatca tcccctcctt ttctgccagc
      241 ggaataaact ctgccggatt cattatttgc ccctgctcac caggccaacg taacaacgct
      301 tcagcgccga tacatttttc tgttttgata tcgattattg gctggtaata aagacaaagt
      361 tgatgttttt cgagggcgcg ttgcaattta cgtttgggag ataaatagtt ttgtcgaata
      421 cgtagccaga gtaatagcag aacaagactc cccaaaatac cggcgggcaa cgcaaaaata
      481 agatgattat aaaaatgggt tataagacgt tgatatgaag tcgcaacaat ggctgcaatt
      541 gggcgttttg tcgaataaac tgtcgcatat aaatagccat ttctttgtac ggttaaatcc
      601 ttcaaatgaa tcagcggaga aaacgttgct gccgaggcct ctttgcttaa cgagaaaaag
      661 gttttcgtca ccgtatcata cacaccccat tgcaatgtcg ggtcatcaga catcacttca
      721 ctccagaaga gagggttgat aaccggcaca taatttcccc gctgcatata ggtcatttta
      781 tagccagaga aaaaaggcgt atcgcggtaa taatagatag aaacgttagg ttcacgctta
      841 taatcggccg gtgcaatcgt atagccgttt acaggcgcta tcagcgatga gcataaaaaa
      901 tggttatcac gggcatagat caattcatta atataaagat agccacgaat aatattcaac
      961 attcgctttt gatgggctgg agtgcaaact tgcccctgat agcgttcagc tgcatcgctc
     1021 actaaatctg cctggcgaat gaccagctcg gatttatcca gtgccagttg agcaaaggtt
     1081 cggagctgat tattcacttc tgattttgcc caaaggaagg caatccatag cgaaagaatg
     1141 atgggaaaga gaactaaaaa gataatgccc ggcaacgcca gtaattggtg tcgtgcacga
     1201 tgactcattg ctatcaattt cctgcctgtt tatacccggc cattgcaagg tgaaaccctg
     1261 gctaccgacg tctttagata aatcttacgg tataaatagc acttttgcct gatttgaaat
     1321 tgataccttt ttaatcgtca cctagatgaa aataattcat tgatattcaa aattaatttt
     1381 ctacaggaat acagaggcgg cgggaagata tgcccttaat gccactgaaa aaccacataa
     1441 tctgtacagc aataaaagtc acggcctaat atggctaaca aacaatcaat tgtgatatag
     1501 ttcacaaaat taatgaaaca aacagagtgt ttcatttttg tgttctgcaa gcccgcgata
     1561 gccagagtgt accacgcctc ccgtgaacaa cgccgcgctg tccagggcat cggctctttt
     1621 cagaggaatt gtttgatggc taccctcacc actggcgtgg ttcttcttcg ctggcaactt
     1681 cttagtgccg taatgatgtt tctggccagc acactcaaca tccgttttcg tcggtctgat
     1741 tatgtcgggc ttgcggtgat cagcagcggt ctgggcgttg tttccgcctg ttggttcgca
     1801 atggggttgc ttggcatcac aatggcggac atcaccgcta tctggcacaa catcgagtcg
     1861 gtgatgatag aagagatgaa tcagacaccg ccacaatggc caatgatttt gacctgatac
     1921 gtagaag
//
LOCUS       DADLSA010000306         1920 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.311, whole genome shotgun sequence.
ACCESSION   DADLSA010000306 DADLSA010000000
VERSION     DADLSA010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.311"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            1..585
                     /locus_tag="JN691_004289"
                     /pseudo
     CDS             1..585
                     /locus_tag="JN691_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(626..1621)
                     /gene="pgl"
                     /locus_tag="JN691_004290"
     CDS             complement(626..1621)
                     /gene="pgl"
                     /locus_tag="JN691_004290"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAY7245506.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFSVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYSVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
BASE COUNT          494 a          513 c          453 g          460 t
ORIGIN      
        1 gtgcggattc tggaacaagg cacgaataat tgcatggaat cggtgctgtg taacgaatca
       61 gactttggca taaacatgaa caacgtcaca aattcatcca ttgattttac cccactggtc
      121 aacgagccat tcgtgctggc ctgtcggcgt gaccatccat tggccaaaaa gtagcttgta
      181 gaatggcagg agttagtggg ttacaaaatg attggcgtac gttcctccag cggcaaccga
      241 ttgttgatag aacaacagct agccgacaag ccctggaagc tggactggtt ttacgaagtg
      301 cgtcatcttt cgacgtcatt aggactagtg gaggcaggac tggggatttc ggcgcttcct
      361 ggtctggcaa tgccccatgc gccctattct tcgattattg gtatcccact ggtggaaccc
      421 gttatacgtc ggacattggg gattattcgc cgcaaagatg ccgtgctttc tccggcagct
      481 gaacgctttt ttgccttgct gattaatttg tggactgacg ataaagacaa tttgtggacc
      541 aatatcgtcg aacgccagag acatgcgtta caggagataa gttaatacac cggcgcagga
      601 gagactgcgc cggttaaatc agcggttagt gtgcgttaac caccacccac attggtccct
      661 gcccgaccgc atagcggcct ttttcatgca gtagcccctg ctcgccaaca atttcgtata
      721 ccgagatgtg gtgagatttt tgcccagcgg caatcagata cttgccgctg taatcaacat
      781 tgaagccgcg cggctgggtt tccgttggct ggaagccttc tttactcaac acgctgccat
      841 cttccgaaac gctgaaaacg gtaatcagac tggcggtacg gtcgcaggcg tataaatggc
      901 gaccatccgg ggtgatatga atatcagccg cccaacgggt gtcggagaag ttttccggca
      961 tcatatccag cgtctggaca cattcgatat tgccgtgcgg atctttcagt tcccagacat
     1021 ccactgagct gtttaactca ttgacgctat acgcatattg ttcgtttgga tggaatacca
     1081 tatgacgcgg gccggcccct tcaacggtag tcacttccgc agggtcctgc gccaccagat
     1141 gaccatcatc gctgaccgta aacaggcaaa tgcgatcctg ctttaatgcc ggaacccaca
     1201 gcgtacggtt gtccggtgag atattggcgg aatggcaacc gtccagcccc tcgaccacat
     1261 cgacgacgcc cactggcagg ccatcttcca gacgcgttac gctcacgtta cccgcattgt
     1321 aagaacctac aaagacaaac tgcccctggt gatcggtgga aatatgcgtc ggactacccg
     1381 gcagcacaga ctctgcggca aaggtcagtg cgccatcgtc cggggcaata cgatacgcca
     1441 ggacgctaaa ctcagggcga acaccaacat agagataacg tttgtccggg ctgaccacca
     1501 tcggctgcac ctgccccggc acatcgacaa cctgtgtcag cgtcagtgcg ccttcatgat
     1561 tcaggttcca gacgtgaatt tgctggctct cagggctggc gatataaact gtttgcttca
     1621 tgaacacacc tcaaaaatga taaatccctt atctttcata cgcataacaa cacttactgg
     1681 ataaaaaaca gacaacaaaa cacgataaaa tacaatatcg cacatcaatt aaatccataa
     1741 gttagcactc catttcgaat ggcatattac taaaatatta gtcaccgaca cgaatttgac
     1801 aatgccacag atattttcgc gactattcag ggagtatgac tctccccgct tttgtttacc
     1861 tgtatcaacg accgtaaaca ctatgccata acaacttctg gccaacttcc cattcgctca
//
LOCUS       DADLSA010000307         1852 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.313, whole genome shotgun sequence.
ACCESSION   DADLSA010000307 DADLSA010000000
VERSION     DADLSA010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.313"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..251)
                     /locus_tag="JN691_004291"
     CDS             complement(<1..251)
                     /locus_tag="JN691_004291"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245507.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIA"
     gene            complement(970..1209)
                     /locus_tag="JN691_004292"
     CDS             complement(970..1209)
                     /locus_tag="JN691_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY7245508.1"
                     /translation="MPQGGVISPLLSNIILNEFDQYLNKRYLSGKARKDRWYWNHSIQ
                     RGRSTAVKENWQWKPAVAYCCYADDCVPRRRVLGT"
     gene            complement(1404..1761)
                     /locus_tag="JN691_004293"
                     /pseudo
     CDS             complement(1404..1761)
                     /locus_tag="JN691_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          425 a          517 c          437 g          473 t
ORIGIN      
        1 gcaatttccg gatacccttc gtcatcaaca cgtacaaacc agaagaccag ttttttgttt
       61 ccggcatcca caaagaacgg aatattcagg tctgcgcaac actcaacggc atcgtcaaaa
      121 ttatcaaagc gcagaacctc agcttcttct tcgtcaaaaa aatcatcttc gtgaagtttt
      181 acgacatagc ggaggagttt gcttctttga ggtgcaggtt ttcgtttgcg tggtttagct
      241 gaacgggcca tataactacc tgaaagacaa tgatattgtc tgtttttata acggtaatca
      301 caggccatga caagccgcag ccgtcaggcc gcctactcgg ggcggtttag aacgcttatt
      361 tagtaccaga tcttacttat aataaagggg cttggcagtt tggataaact attgtcctta
      421 ttccactaat acaaactttt tgtaaagtat attaaatata cttcatttca tactgaaatc
      481 cccggaagta ttcaatcaaa caattacacc ataaaaggaa gaggggcccc aattaagttg
      541 tcaagcatgt ttactgcgca atcgcttggt gctccgtccc tgtaccccgc attggtattc
      601 tggcgctcat ggggcttcca cttgcgcttc tcccttcaca tcggggtgac aggtccacgt
      661 tccccacaag agcctgaatc gacttcacgc cgcttttatg ccggatgccg cgcaggcagt
      721 aaacatatcc cacaggaacg actccaccat tgttttgaca tcatctatat gctttcgaca
      781 catcatcagc ggttcacttt cgttcgtctc ttcgaaccat acctgatcgg ctcacgtccg
      841 accttttccc atctcgctca ccaccacatc tcttaaatgc agcagcagtg gatggtttgg
      901 tgcctgcgcc tgcacgccga caccgagggg ccttccctca tctcttgtgc agcatggctg
      961 cacagggact cacgtcccca ggacgcgcct tcgtggcaca cagtcatcgg catagcaaca
     1021 gtaagcaacg gcaggtttcc attgccagtt ttctttgaca gcagtacttc ggcctcgctg
     1081 gatactgtga ttccagtacc agcgatcctt tctggctttt ccactcaggt agcgtttatt
     1141 cagatactgg tcgaactcat tcagtatgat gttcgacagc aacggcgaga tcacaccgcc
     1201 ctgtggcata ctttcactga ccgcccggaa gagaccaaca tcaacacggc gttttccaca
     1261 gcagtctcat aaaacgtgca tcactgatcc tgcggcagac ggctttcatc agcagccggt
     1321 gatgtactgt gtcgaagtag ctggacaggt caccttcaat aacccggcgc cccgtgtttc
     1381 cccgcagtcg gtgagctgta atagtacgtg atgtctcccg cccacttctg gttcgggcca
     1441 ctggcgtaaa aatcctgcgt cagcagattt tctgatacag gcaggccgtg tgcacggtaa
     1501 ctgatcgggc tgaacttctg ggaggctttt gcgctcagtt cctgacggcg cagactggct
     1561 gccacggttt ttacgttaaa gtggtaacca gagcacacag ttcatccgtc aggcgtgggg
     1621 caccgtaacg ctggactggg taaaagcctt gcggacaacg ctgtcgcagt gctgacggaa
     1681 ctgctgacgt gtgtttatcc ttgtccgccg ctgacaccac gtataccagc cgctgcgggc
     1741 cacccggagt acgcggcaca ttgccttgat gacggtttac gcggtcgcct gcggcaacgg
     1801 ctttccggct gacggtagtg tggacctttt taccccggag aacaccggca ag
//
LOCUS       DADLSA010000308         1847 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.314, whole genome shotgun sequence.
ACCESSION   DADLSA010000308 DADLSA010000000
VERSION     DADLSA010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.314"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..804)
                     /gene="ipaH9.8"
                     /locus_tag="JN691_004294"
     CDS             complement(<1..804)
                     /gene="ipaH9.8"
                     /locus_tag="JN691_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY7245509.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPQIYFSMSDGQQNTLHRPLADAVTAWFPENKQS"
     gene            complement(1066..1161)
                     /locus_tag="JN691_004295"
                     /pseudo
     CDS             complement(1066..1161)
                     /locus_tag="JN691_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1197..1502)
                     /gene="ccdB"
                     /locus_tag="JN691_004296"
     CDS             complement(1197..1502)
                     /gene="ccdB"
                     /locus_tag="JN691_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HAY7245510.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1504..1722)
                     /gene="ccdA"
                     /locus_tag="JN691_004297"
     CDS             complement(1504..1722)
                     /gene="ccdA"
                     /locus_tag="JN691_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HAY7245511.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
BASE COUNT          503 a          382 c          418 g          544 t
ORIGIN      
        1 agattgtttg ttttccggga accatgctgt cacggcatca gccagggggc gatggagtgt
       61 attctgttgt ccgtcactca tggagaagta aatctgcggg ccgtggtagt ccggcgaaga
      121 ggttaatctt tgcagggctt gcagagcatg ggatgataat gggttatcac taatatgtat
      181 tgaacattca ttcctcagat taagaatgct ttccgggata tgacttatct gatttctatc
      241 aaaaaaatac tctctgacaa cgggattatt tccctcagaa aatgctggta gctcagtgag
      301 gaaattacgg gtcgctctga gatttttaag agcctgtggg agcgatggta aggagactat
      361 ttcgttatag ctgatattca tagtcaataa cgaatcgggt aaagctggca ggttttccag
      421 ctcattgtgt tgtacctgaa gtgactccag cgcaggaggt agcacgggca attctgataa
      481 tttattgctg gcggaatata atttttttag cgtaacaggc agttcaggta ggttagttaa
      541 ttgattatat gatacattga gcagcgttat ctgagctggt aagttgtcag gtagcgagga
      601 cagatttaaa cggtccagtc gaagttcatc ggaattattg ataagacatt ctttaagtcg
      661 ggagacagcc tcatcacgct cttcaccggg gagcgcttgt ttttcccatt tatcccatgc
      721 tgaaaagtaa tcagcatatg taccggaaat agtgttataa aaagaatttt ggggcaatga
      781 aaagttatta tttatcggta acatgatggg catccagtta aagccggtgg acaaatggct
      841 ttgttaaaag aaataagtag gagtttttat gaagttcatt ccatgaaatg aacttcagca
      901 cttttttatt ataataagga taaacaaaat tggctgtaaa aaaacgatac tgtttcagtt
      961 aaccgtgaat ggcctgtttg caccgtattg ctcttgtttt tcggttagtt aaacatcgta
     1021 cttcatattt gaacggtaag cggctcgtca gaaccgtatt gatatttact gagaggtcag
     1081 atcaactttc cagggcaaca gatcgcgtac ccggtttgcc ggccagtcct ggatatgctc
     1141 aatgacgtaa cgcagccatt ttgtcaacgc cacgattttt gaccctagcc tgacatttat
     1201 attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca
     1261 gccacttctt ccccgataac gaagatcggc acactggcca tatcagtggt catcatgcgc
     1321 cagctttcat ccccgacatg caccaccggg taaagctcac gggagacttt atctgacagc
     1381 aggcgggcac tggccagggg gatcaccatc cgtcgtccgg gcgtgtcaat aatgtcactc
     1441 tgtacatcca caaacagacg ataacgactc tctcttttat aggcgtaaac cttaaactgc
     1501 atttcaccag tccctgttct catcggcaaa agagccgttc atttcaataa aacgggcgat
     1561 ctcagccatc ccctgctgat tttcagcctg ccagcgttcc gcacgcagac ggcgggcctc
     1621 tttttgcatg gccgtattta ccaggcctga gatattcacg tttgcggatt taagtaactg
     1681 atagctgtca ctgtctatag tgacagtaat acgctgcttc atgataagcc ctcaaaaaca
     1741 tacttatgat atgtatatga gtatatatta atggtacaaa ccatcaatat ccataaaaag
     1801 tacaccatta attcgctata agtttttaat acaatttttt ctgctgc
//
LOCUS       DADLSA010000309         1810 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.315, whole genome shotgun sequence.
ACCESSION   DADLSA010000309 DADLSA010000000
VERSION     DADLSA010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.315"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(88..561)
                     /locus_tag="JN691_004298"
     CDS             complement(88..561)
                     /locus_tag="JN691_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HAY7245512.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            717..1730
                     /gene="intI1"
                     /locus_tag="JN691_004299"
     CDS             717..1730
                     /gene="intI1"
                     /locus_tag="JN691_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY7245513.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
BASE COUNT          381 a          510 c          499 g          420 t
ORIGIN      
        1 aatattcaca taacgtcgct atcaatcgcg gaaaaggcgt agtgcgcgaa gcgaacgaag
       61 ctttttgccg tcgattgcat agctttgtta accctttttc caaatttgat agcaatagtt
      121 aatgtttgaa gtaaagtgtt gctcaaaaac aacttcgaag gtatttggaa tactcgggaa
      181 gaaaacatcc ccctctggct cgatgtcgat cgtcgataag tggagcgtag aggccatggg
      241 taatgtttct cggtaaattt ctccgccacc agacactata acgtgaccgg tgaattcagc
      301 tagcctgtcc atggcctctt cgattgactg aaatacaact acattgtcat catttgatgt
      361 ccaacctgag cgggtaacga ccgcgtattt cctattgggg agtgcgccca tagattcaaa
      421 cgtcttgcga cccaccagaa gccactgatt gtaggtcaat gctttaaaaa gtagctgctc
      481 ccctttcgcg gaccagggta tgtctggacc gcaaccaatc acgccgtttt tcgctttcgc
      541 agccatcaat gatactttca aggttctcat cctgggttaa ctttgtttta gggcgactgc
      601 cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
      661 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
      721 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
      781 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc cactgggttc
      841 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
      901 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
      961 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     1021 aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     1081 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag cttctgtatg
     1141 gaacgggcat gcggatcagt gagggtttgc aactgcgggt caaggatctg gatttcgatc
     1201 acggcacgat catcgtgcgg gagggcaagg gctccaagga tcgggccttg atgttacccg
     1261 agagcttggc acccagcctg cgcgagcagc tgtcgcgtgc acgggcatgg tggctgaagg
     1321 accaggccga gggccgcagc ggcgttgcgc ttcccgacgc ccttgagcgg aagtatccgc
     1381 gcgccgggca ttcctggccg tggttctggg tttttgcgca gcacacgcat tcgaccgatc
     1441 cacggagcgg tgtcgtgcgt cgccatcaca tgtatgacca gacctttcag cgcgccttca
     1501 aacgtgccgt agaacaagca ggcatcacga agcccgccac accgcacacc ctccgccact
     1561 cgttcgcgac ggccttgctc cgcagcggtt acgacattcg aaccgtgcag gatctgctcg
     1621 gccattccga cgtctctacg acgatgattt acacgcatgt gctgaaagtt ggcggtgccg
     1681 gagtgcgctc accgcttgat gcgctgccgc ccctcactag tgagaggtag ggcagcgcaa
     1741 gtcaatcctg gcggattcac tacccctgcg cgaaggccat cggtgccgca tcgaacggcc
     1801 ggttgcggaa
//
LOCUS       DADLSA010000310         1808 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.316, whole genome shotgun sequence.
ACCESSION   DADLSA010000310 DADLSA010000000
VERSION     DADLSA010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.316"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..849)
                     /locus_tag="JN691_004300"
     CDS             complement(<1..849)
                     /locus_tag="JN691_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7245514.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIHSLTPFSLPMDLTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
     gene            complement(1238..1504)
                     /locus_tag="JN691_004301"
     CDS             complement(1238..1504)
                     /locus_tag="JN691_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245515.1"
                     /translation="MLTQTIFPCLPQKQENISLEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(1613..1808)
                     /locus_tag="JN691_004302"
                     /pseudo
     CDS             complement(1613..1808)
                     /locus_tag="JN691_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          505 a          296 c          380 g          627 t
ORIGIN      
        1 gtgttctgga gcggaaattg tggcagaatt tcccatagag aaaaatattc gaggaccaga
       61 ataatctggt ccggtggtta tttgttgtag tgtacgcatg gtatggccag acaatggatt
      121 acctgcaaca tttacgaagg cattctgagc taatctcagg acactttccg gaagtgttgt
      181 cagattatta ttcattagca cagccctgtt catactaaaa ggtaattccg gtagttgttc
      241 tataaaatta ttagccagag ccagcccttc aagcttatct ggaaaggggg gaatgctgtc
      301 taatctgttt ccagaaacta ccagtaattt tagttccggt ggcaatagtg gcagaatatg
      361 caatcggtta ttatgaaccc gtaaatgagt tagattttca ggaagaaatg gtaattcttt
      421 tatcttgtta tcacccgcat taagctcttc taacgtctcg ggtaaggcag gtaagctttc
      481 cagttgatta aattgagcat gtattacctt aagcattggt ggtaaatcag ggagatgtgt
      541 taataggttt tttcttattt ccagtgttgt tatctgaggc ggtaaatctg gtaatgtttt
      601 taaatctaac tcagataaat ttaattctgc ctcttgattt tgtaaacata ttttcagcct
      661 attgaaggcg atattgcgtt gctcgcctgg ggtaccgttc ttttcccatt cactccaggc
      721 ttttaaataa aattcattac aagatggagt cagatccata ggtaaagaaa atggagttaa
      781 cgagtgtata ttgtttattt gatgcataat accagaaccg attgcctgta tattggttga
      841 tttaatcatt ataactagaa ttcttatcaa cgataagaat tctagtgaaa tagaatatag
      901 cttattaatt ttaaaacagt ttgctttgta ccctttctcc gggtattgct ttccccctaa
      961 gccgtctatg ggaaggagtc tgctgttatt cctgagaatg gtaattcagc cataaaaagg
     1021 tatttaaggt cattgcgtct ctccctgtac tcgaataatg gtgaagaaag catgatgaat
     1081 atatcacgat aacgttcagt acgtatcgag gataaatgga gttactcctc tctgaaatag
     1141 aattactgct ctattttttt ctgttttcag aagttattag taacaaaccc tgatatattt
     1201 ttttgaaaat ggagcttaaa cgagtctgaa attaacttca gaaatcagaa taccgttggc
     1261 tttgtttttc atcaattccg gcttcattta tctttgtcaa ccattgcatt tctgtagcag
     1321 tgaatttcaa cgttttcgtc cggttggcag caggtatttg taattcgtat atagctgctt
     1381 ttttattgaa aacagtatat ccatctgtag taacagtaga agataataat acaggatttg
     1441 atacttcaag gctgatattt tcttgttttt gtggaaggca agggaatata gtttgtgtaa
     1501 gcatattact tccttatgtt atattttggt aaaaaattta tttttttaca ttatagtcgg
     1561 ctaaatgaag tttactgtaa aaaaacgata ctttttcggg tggcgcttcc aatcagtaga
     1621 atatgtgttt ttcaatgaag gagccgatga cttttcgtgg atttccacgg aacgcgagaa
     1681 gcagattgtt ttacgtgcca gccgcttgat gcgggtgcgg agggtcaggt tgttacgttc
     1741 aagatatagc atgtgctgga tgaagatccc ttttctggtc acctgtttat ctttcgtggc
     1801 cgacgggg
//
LOCUS       DADLSA010000311         1792 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.317, whole genome shotgun sequence.
ACCESSION   DADLSA010000311 DADLSA010000000
VERSION     DADLSA010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.317"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            461..1543
                     /locus_tag="JN691_004303"
     CDS             461..1543
                     /locus_tag="JN691_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HAY7245516.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
BASE COUNT          566 a          365 c          336 g          525 t
ORIGIN      
        1 acatgcccca tcacatcctc aacatcaaag tattagagcc ttgcaaacca ttatgcgtct
       61 cagtttgtct catctatctt acgcaaaaaa aatagaacta atatcaagcg gcgctatatt
      121 gcgtattaaa taaaaccacc tcaattacat taataaatct aaataaatat catgcaaata
      181 atttccatgg ttatttttag gctatttaaa ttaaaaaaat aaattatgag tcaatcataa
      241 ataacaacct ttaatataat ttgactacca aatttatatt cgttcacaaa atagtcatgc
      301 attgtgtgca atagaaacag ttcagataaa aatagggatc tacttcacaa tttaaatgag
      361 aaactaaaac ttacatcttg aaataatctc attgattgga tgaatatttg tagtgcagtg
      421 acatcatttt taaataatag ttcaaataaa agggctcacg atgaaaaaat taacagtggc
      481 aatttctgct gtagctgcat cagtactgat ggcgatgtct gctcaggcag cagagattta
      541 caataaagac agtaacaagc tggatctgta cggaaaagtt aatgctaagc actacttctc
      601 ttctaacgat gcagatgatg gcgacacaac ttatgttcgt ctgggcttca aaggcgaaac
      661 tcaaatcaac gatcaactga ctggtttcgg tcagtgggaa tatgaattca aaggcaaccg
      721 tgctgaatct caaggttctt ccaaagataa aacccgtctt gccttcgctg gcctgaaatt
      781 cggtgactac ggctccatcg attatggccg taactacggt gtagcatacg atatcggtgc
      841 atggaccgac gttctgccag aattcggtgg cgatacctgg acccaaacag atgtgttcat
      901 gactggtcgc accacaggtg ttgcaaccta tcgtaacaac gacttctttg gtctggttga
      961 tggtctgaac tttgctgctc agtatcaggg taaaaatgac cgcactgacg taactgaagc
     1021 taacggtgat ggtttcggtt tctccactac ttatgagtat gaaggattcg gtgtaggtgc
     1081 aacctatgct aaatctgacc gcactaatga tcaggttatc tacggtaaca acagcctgaa
     1141 tgcatctggt caaaatgctg aagtatgggc agctggtctg aaatatgatg cgaacaacat
     1201 ctatctggct accacctatt ctgaaaccca gaacatgact gtttttggta ataaccatat
     1261 tgccaacaaa gcacaaaact tcgaagtagt tgcacaatat cagttcgact tcggtctgcg
     1321 tccgtccgtt gcttacctgc aatctaaagg aaaagacttg ggtgcgtggg gtgatcagga
     1381 cctgattgaa tatattgatg taggtgcaac ctattacttc aacaaaaata tgtccacttt
     1441 tgttgattac aaaatcaacc tgattgataa gagcgatttc acgaaagcat ctggcgttgc
     1501 taccgatgat atcgttgctg taggattggt ttaccagttc taatctgatt actcaaagat
     1561 atgttgtggg aggctttgcc tccccaacat ataagtggcg ccctcaagcc actttcctta
     1621 gaagcactat cttgcttctg actatataaa ccttctgtta tatattaccc tttattttct
     1681 gggacgtttc aacgccccat ttttaataat actcagtgaa caattaacgt atcaattaga
     1741 tttaataaca acgatatccg tacctaacct gatatctaat gtcataagca tc
//
LOCUS       DADLSA010000312         1773 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.318, whole genome shotgun sequence.
ACCESSION   DADLSA010000312 DADLSA010000000
VERSION     DADLSA010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.318"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..201
                     /locus_tag="JN691_004304"
     CDS             <1..201
                     /locus_tag="JN691_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245517.1"
                     /translation="YEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIY
                     RQLTDEVLALRLSENGSQLHHS"
     gene            409..678
                     /locus_tag="JN691_004305"
                     /pseudo
     CDS             409..678
                     /locus_tag="JN691_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            675..787
                     /locus_tag="JN691_004306"
                     /pseudo
     CDS             675..787
                     /locus_tag="JN691_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970002.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            930..1520
                     /gene="ospG"
                     /locus_tag="JN691_004307"
     CDS             930..1520
                     /gene="ospG"
                     /locus_tag="JN691_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HAY7245518.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          515 a          365 c          440 g          453 t
ORIGIN      
        1 tatgagatgc tggagaatga gtaccctcag agggtggctg accggctgaa agcatcaggt
       61 ctgagcggtg atgcggatgc ggagagggaa gccggtgcac aggtgatgcg tgagactgaa
      121 cagcagattt accgtcagct gactgacgag gtactggccc tgcgattgtc tgaaaacggc
      181 tcacaactgc accattcata atcacatcgc ataaaactgt cagcgccagt gataggacag
      241 gtgaaacctg tcaatacgta ccggcttacg ccgggcttcg gcggttttac tcctctgcca
      301 tatgcaacaa cggagtgccg ccttacatgc cgctggcgcg gcatatcctg gtgacgatat
      361 ctgaacagtt atatactgtg tatatacgtg gtaatgacga ggtggcaaat ggcacaggtt
      421 aatatgagtg taagaatcga cgcagaactg aaggatgctt ttatggccgc tgcaaaaagc
      481 atggaccgta atggctctca gttaatccgg gattttatgc gccagaccgt tgaacggcag
      541 cacaattctt ggttccgtga ccaggttgcg gcaggccgtc agtaactcga atgcggtgat
      601 gtgcttcccc atgacatggt cgaatcctct gctgctgcat ggcgggatga aatgagcagg
      661 aagattgctg ataaatgatg gagatgttct ggaccatgct ggccatccag gacaggaagc
      721 gcattcgtag tacattgcgg agcagaacct gatggccgcc attgaaccgg acgaacggat
      781 tggttattca gccagcagcc tggcagggca gccgtataaa ggccgcaacg gtcgtgtgga
      841 aggaacatct ggaccacaca aggtagcgtg taacgttatt ttgtgtgaaa atctcttgtg
      901 atttttaaat atcgtaacaa aggtatgaca tgaaaataac atctaccatt attcaaacac
      961 cttttccatt tgagaataat aattctcatg ctggcatagt aacggagccc attctcggta
     1021 agttaatagg tcaggggtcg acagcagaaa tctttgaaga tgtgaatgat tcatctgctt
     1081 tgtataaaaa gtatgatctt attggcaacc agtacaatga gattctggaa atggcttggc
     1141 aagaatctga gctttttaat gctttttatg gcgatgaagc atccgttgtt atacagtatg
     1201 gcggagatgt gtacctccga atgctgcgcg tgcctgggac tccccttagt gacattgata
     1261 cagctgatat ccctgataat atagagagcc tttatctaca gttgatatgt aaattgaatg
     1321 agttgagtat aatccattac gatcttaata caggtaatat gctgtatgat aaagaaagtg
     1381 aaagtttatt cccaatagat tttcgcaata tttatgctga atattacgct gcaaccaaaa
     1441 aagataaaga gattatcgac cgacgattac aaatgcgtac aaatgatttt tattcgttat
     1501 taaacaggaa atatttatag ccgtatttgt tgatgctata agccagaggc tcttctatga
     1561 gaatatgggg accattccct cgcgatatta acgtaatatc ttaattgtat gcaccgccag
     1621 aatgcgttca gtgcgatcac ggcagcagac aggtaaaaat agcaacaaac cacccgaaaa
     1681 accgccgcga tcgcgtatga taaattttaa ccacatgcat acctgtgcag caaggcgaat
     1741 cacgcacgga tttccagcca gagacacctg aaa
//
LOCUS       DADLSA010000313         1696 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.319, whole genome shotgun sequence.
ACCESSION   DADLSA010000313 DADLSA010000000
VERSION     DADLSA010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.319"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..121)
                     /locus_tag="JN691_004308"
     CDS             complement(<1..121)
                     /locus_tag="JN691_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7245519.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILE"
     gene            449..524
                     /locus_tag="JN691_004309"
     tRNA            449..524
                     /locus_tag="JN691_004309"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:482..484,aa:Asn,seq:gtt)
     gene            complement(580..1515)
                     /gene="ldtA"
                     /locus_tag="JN691_004310"
     CDS             complement(580..1515)
                     /gene="ldtA"
                     /locus_tag="JN691_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7245520.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(1577..>1696)
                     /gene="cobT"
                     /locus_tag="JN691_004311"
     CDS             complement(1577..>1696)
                     /gene="cobT"
                     /locus_tag="JN691_004311"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="catalyzes the synthesis of
                     alpha-ribazole-5'-phosphate from nicotinate mononucleotide
                     and 5,6-dimethylbenzimidazole; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY7245521.1"
                     /translation="GAALAMSIIEAACAIYNNMGELAASNIVLPGNTTSDLNS"
BASE COUNT          449 a          428 c          372 g          447 t
ORIGIN      
        1 cttccagtat ggcaacctgc tggctgagcg ctggttgtgc gatatgcaat acttcagcag
       61 cctgggtcag gctaccaata tctacaattt ttacgaagta tttcaggcgt ctgaagttca
      121 tgttgcctcc ggtttttaag aatcggccca agtgccgcca ttacttacaa ccagtatgca
      181 agatgcctgc cagttctatt ttggcgttga tgcgcaagct aaccaactgt caaataagag
      241 attatgatag attcgtcatt ggcccttttt atcagcagtc gcattcacct gccctataaa
      301 aggaaggtat gcaccacgat ggttcattac ccaataagat tgaaagctca ccactttgtt
      361 gaaattggca gcaaacaaac aaaaaaaatg catttcaccc tttgacatca ccatgcactg
      421 ccattaatat gcgccccgtt cacacgattc ctctgtagtt cagtcggtag aacggcggac
      481 tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaaattca aaaaagcctg
      541 ctttcgagca ggctttttac tttctaatta ccaacgctct taaaacatct gtcttgaacc
      601 agaactaatt tgcacaggca ttcccgatcg atgttgcaac gcagcatttg cgcgatttac
      661 atcaacttct tgcccgttga taaacgcccg caaagatggg gttaccggca atggcacttt
      721 tcggtcagac tcatattctg cacgattgcg cgacagcggc tcatgaactt ccagccactt
      781 tgaaccatcc ggttccgttg tgtatttcac tggctgatca ataatttgta cacgagtgcc
      841 tacaggaaca ttatcaaaca gatatttgat atcgtcatta cgcagacgaa tacagccctg
      901 acttacacgg agcccaatac caaaattggc attggtaccg tggatggcat acaacctgcc
      961 aatataaatc gcgtacagcc ccatggggtt atcgggcccc gcaggaacaa atgcgggcaa
     1021 actctcccct cgtttcgcat attcgcgccg ggtgttcggc gttggcgtcc aggttggtgc
     1081 ttcttgttta cgttcaacgg tagtcaccca gttacgcggg gtttctcgcc cagcctggcc
     1141 gataccaata ggaaagactt ccactgtatt actgtctggc ggatagtaat aaagacgcat
     1201 ttcagcgacg ttaaccacaa tccctttacg aacagtggcg ggcaaaatca gttgctgcgg
     1261 aatggtgagt tgtgagccag gcttcggcaa aaaaacatca gcgcccggat tcgcttccag
     1321 catattactt aacccttgcc cgtattgtgc ggcaaaagtc tccagcggct gagtattgtg
     1381 atcaggaaca gttacagtaa gcgactgccc cactaaacgg ctaccctctg gaggtaatgg
     1441 ataagttacc gccaggctat tttggctggc aaaaagcaga gcaaatgagc aaagaatatt
     1501 tacacgacgc atcatgtccc tttcctaagt cgcgaatact atccgataag tatagctttt
     1561 atcagacttt tcgtttttag ctgttcaaat cagaagtcgt attccccggt agaacaatat
     1621 tactggcagc aagttcgccc atgttgttgt atatcgcaca agcagcttcg atgatggaca
     1681 tcgccagagc tgcacc
//
LOCUS       DADLSA010000314         1635 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.320, whole genome shotgun sequence.
ACCESSION   DADLSA010000314 DADLSA010000000
VERSION     DADLSA010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.320"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(1..1005)
                     /locus_tag="JN691_004312"
                     /pseudo
     CDS             complement(1..1005)
                     /locus_tag="JN691_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481272.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            complement(1340..1558)
                     /locus_tag="JN691_004313"
     CDS             complement(1340..1558)
                     /locus_tag="JN691_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245522.1"
                     /translation="MFKCNCTLIVGGIMKNALQFLFVAFWLFASCMPIIFTARYMEKV
                     DVLILIFGYINALFLGVFMAVMCIEYWR"
BASE COUNT          447 a          390 c          348 g          450 t
ORIGIN      
        1 gcactgcttt tgtactggaa agtgagattt ttagacagaa cgcgcttcgc ctgtaggctg
       61 aagatatacc ccagttcctc ttcagaatgg tgcacatcaa gatgagcatt atcagtagta
      121 cgaggcgacg tagcgaaccg gttgttatag gcttcaataa aggtcggcaa ccatgcattt
      181 gctgtttcaa tatcactgat attctgaagc cgcatttctt tgaccagcct gtcctgtagt
      241 gtctgattgg cgcgctctac ccgccctttt gcctgcgggc tgttggcatg gattggctcg
      301 atgcccagtg tctttatcgc acgagtgaac tgggtcagct caccttcccg ctctgggtta
      361 tttaccctga atatactgtg tctatcagag tagagagcga gcggtacgcc atgatcatta
      421 aggtaacccc ggagggtttc catgtaagcc cgggttgttt cagcaggcac aaaacgcaac
      481 gccatcaacg cactggtggc atcatcaatg aaaacgatca gtgtacatct ggggcctcga
      541 ttttcaaacc agtcatgagg tgagccatca atctggatca gttcaccgta agatggtcgt
      601 cgcatgcggc gttgatatat acgggcaatt ttacgacggc gttcacgcca taccccctct
      661 tctatcatcc actttctgag agtttcaacg gataaagata aaccgtgtat ctcgcgcaat
      721 ttttcgcacg caaacgtagg tccaaaatca gcgtaacggc ctttgaggag tgaaattact
      781 gttgctctga attcagtaga aaaggaatta ttaggacgct ttccacgtcg gtgggaaacc
      841 agaccagaag gcccttcatt tctataccgt tgcaccagac gtttaactag aaatgccaat
      901 gcgtgccgca gcttgttcct gagtaatatg tcgattaatt gactcctgaa taatctgaag
      961 tcggtggagt tccttatgac tcatcgtaac agtctctttg atcatgaaaa atcccccaga
     1021 gaattgtctg gggacatttt agaatggttc aaaggggaca ttacagcttg gtgttaacaa
     1081 tagcctgctt ctccttgcct ttcggcacgt aagaggctaa cctacatgtg tctggcatga
     1141 aattggcctc agattaatgt taagcgtctt gcaggacgcg taatgttaac tggggctttt
     1201 ctctacctgc cgttggtgtt cgtgcccgag gcagatagcc tcaagcaccc gcagtcattc
     1261 tacttaacta agatttcccc gcaaaccgtt tttgtccagc acagtaaata tccaactaaa
     1321 ccaatggagt tcgctgtatt taccgccagt attcaatgca catgaccgcc atgaacaccc
     1381 ctaaaaaaag ggcatttata tatccaaata ttaatatcaa aacatcaact ttttccatat
     1441 accttgctgt gaagatgatg ggcatacatg atgcgaacaa ccagaacgca acaaacaaaa
     1501 actgcaatgc gtttttcatt attcctccta caatcaatgt gcaattacat ttaaacacac
     1561 ctcaatttgg ccggacatat aaatatctaa accagaaaaa atcacttaca tagcgttaca
     1621 aactctttag tctaa
//
LOCUS       DADLSA010000315         1630 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.321, whole genome shotgun sequence.
ACCESSION   DADLSA010000315 DADLSA010000000
VERSION     DADLSA010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.321"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..1046)
                     /locus_tag="JN691_004314"
     CDS             complement(<1..1046)
                     /locus_tag="JN691_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HAY7245523.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1538..>1630
                     /locus_tag="JN691_004315"
     CDS             1538..>1630
                     /locus_tag="JN691_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HAY7245524.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIV"
BASE COUNT          480 a          270 c          346 g          534 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaggac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atcttaatgt actgtttttt tctttaaaaa aacaatacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattct ccccccccga
     1501 cttttgacgc agtgtcttgt taacaggggg atttaccatg cttattggct atgtacgcgt
     1561 atcaacaaat gaccagaata ccgatctaca acgtaatgcg tttatttgtg ccggaagtga
     1621 gttgattgtt
//
LOCUS       DADLSA010000316         1625 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.322, whole genome shotgun sequence.
ACCESSION   DADLSA010000316 DADLSA010000000
VERSION     DADLSA010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.322"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(172..1359)
                     /locus_tag="JN691_004316"
     CDS             complement(172..1359)
                     /locus_tag="JN691_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7245525.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPLVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQTDVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKLPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(1359..>1625)
                     /locus_tag="JN691_004317"
     CDS             complement(1359..>1625)
                     /locus_tag="JN691_004317"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245526.1"
                     /translation="EEDAEVLRFDSFDDAVECCADLNIPFFVDAGNKKLVFWFVRVDD
                     EGYPEIARCTEREFATILAGISAGGMYCPECGTVHWPDGVAPPF"
BASE COUNT          380 a          501 c          373 g          371 t
ORIGIN      
        1 ccccaaaaag attaaaaaaa caccacaaaa cggatgtttc ttcaacacca cttttgctcc
       61 atatgaacgg aaccgacgat taaactggat ggctctgatt gattcagggt atgaatggcg
      121 gttttttgct ccgtttccct caaaatggac gcaacttccc ctctgcggct ctcagccgca
      181 ccaccgcatc cgggccagcg gctcatgcat caggacctgc tctgccagac ggtagccccg
      241 cttcagcccc gtaaaacgca tctgactccc gcacagcacg cacttcagcg ggtcaacctt
      301 cagtaacctc tgatacatcc ctctccaggt gatttgcatc gccgtttttc tcactgtctc
      361 cgttatgatg tacaccactt cttccagtaa ccgccgtttc gccggactca aaaaaccgta
      421 gtacctcacc atacggaacc ccttatccgc cacatgccag gagaaccttt ccatgaactc
      481 atctccactc atcaacaggt attcttcccg ttttgttcgg tgactgttgt aacgcagacc
      541 gatttcatcc tgaccggcat aatgctccag acgactcatc ggcaccggta gctttttcag
      601 gtaagagcca aagtacaccg ccacatgggt ggcattatcc atcacccggg atacgttgac
      661 attccagcca cggcggtaat gcgtgtccag gaagcgattc cattcccgtt tactgcttcc
      721 ttctgctgcc agcgcatccg gcatcaccag gtcagggtat ttccgtgaca gcaaccgtgt
      781 tatccggtag cgccacatgc tcatcacctt acgggcgtaa aaatgaagat ttttccaggt
      841 gtggcccgac gtcacaccac cggcagttgt cgataaatgg atatgcggat gccactgctg
      901 gtcacgcccc catgtgtgga tcaccgtgaa tatccccggc tccacatctg tctgatggca
      961 gatttccagt atcacatccg ctgcaatgcg gctcatctct gccagtaacc accggttgtg
     1021 gaacaccagg gaccagtact ggcagggaag tgtgaacaca atatgctgcc acgggcagtc
     1081 ggggaccagg ctcagcagat actgtatcca ctgtgcgcca gccttcaccc cgcagtgcgg
     1141 gcaggagcgg cttttacacc ggaagcagac cttttttgta tggcaacagt ccggtgatga
     1201 acaacaccac tgcgtgtagc ccatcagtgt ggtcccgcag gccatgattt ttgtcactga
     1261 ttcaattacc aacggacgga ctgccccttc cggttgtttc tccagccagt taagccagcg
     1321 gtttccctgc tgaaaaatat cggcaaaacg gggaagcatc agaagggcgg ggcgactccg
     1381 tccggccagt gaaccgtgcc acactccggg cagtacatac cgccggcgct gataccggca
     1441 agaatggtcg caaattcccg ctccgtgcag cgggcgattt ccggataccc ttcgtcatca
     1501 acacgtacaa accagaagac cagctttttg tttcccgcat ccacaaagaa cggaatattc
     1561 aggtctgcgc agcattcaac ggcatcgtca aaactatcaa agcgcagaac ttctgcgtct
     1621 tcttc
//
LOCUS       DADLSA010000317         1616 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.323, whole genome shotgun sequence.
ACCESSION   DADLSA010000317 DADLSA010000000
VERSION     DADLSA010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.323"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..196
                     /locus_tag="JN691_004318"
     CDS             <1..196
                     /locus_tag="JN691_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HAY7245527.1"
                     /translation="ALNQLLEARTQLANLQTYMDGKAGAENLINKLLQDPTLLKALAS
                     APKPVAQQESVLSGETGTAD"
     gene            221..>1616
                     /gene="tssC"
                     /locus_tag="JN691_004319"
     CDS             221..>1616
                     /gene="tssC"
                     /locus_tag="JN691_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HAY7245528.1"
                     /translation="MVNDTLLATGDLSAAQTTAYQDTEFDSLLDKAFRPKTPQAAKAV
                     EAAVQTLAQQALTNTVTVSDDAYKSIAAIIEQIDFKLTEQIKLILSHPEFQKLESSWR
                     GMEHLVYNTETDEKLKIRFMNLSKDELRKNMKRYKGIAWDQSPMFKKLYEAEYGQLGG
                     EPYGCIIADYYFDHTPPDVDLLGSIAKVAASAHAPFIAGASPSVLQMDSWQELANPRD
                     LIKIVTQNLEYAPWNSLRKSEDSRYIGLTMPRFLSRLPYGAKTNPVDEFDFEEDADGA
                     DHSKYVWSNAAYAMGVNINRSFKHYGWCTLIRGVESGGAVENLPCHTFPTDDGGVDMK
                     CPTEIAISDRREAELAKNGFIPLIHRKNSDYAAFIGAQSLQKPQEYYDPDATANANLS
                     ARLPYLFACSRFAHFLKCIVRDKIGSFKEREDMQRWLNEWIMNYVDADPVNSSQETKA
                     RRPLAAAEVVVEEVE"
BASE COUNT          436 a          389 c          395 g          396 t
ORIGIN      
        1 tgccctgaac cagctgctgg aagcgcgtac tcagcttgcc aacctacaaa cgtatatgga
       61 tggtaaagcg ggagcagaaa atcttatcaa caaactatta caagacccta cgttactgaa
      121 agctctggcc agtgcgccaa aacctgtcgc tcaacaggaa agtgtcttat caggtgaaac
      181 gggaacagca gactaactgc gattttatta aggaactttc atggtaaacg atactcttct
      241 ggcgactggc gatctctctg ctgcgcaaac cactgcctat caggacactg aatttgattc
      301 gctcctggat aaagccttcc gtcccaagac acctcaggca gcaaaggctg ttgaggcagc
      361 agttcagacg ctggcccaac aggctctgac aaataccgtc accgtcagtg acgacgctta
      421 taaaagtatt gctgcgatta ttgagcagat tgatttcaaa ttaactgagc agatcaaact
      481 catcctgagt catccggaat ttcagaaact ggagtcctcc tggcgcggga tggaacattt
      541 ggtttacaac actgaaaccg atgaaaaatt aaaaatccgc tttatgaatc tttcaaagga
      601 tgaattgcgg aaaaatatga agcgttacaa agggatcgcg tgggatcaaa gccctatgtt
      661 caaaaagctt tatgaagctg aatatggaca gttaggtgga gaaccttacg gttgtattat
      721 tgcggattat tattttgatc acacgccacc ggatgtggat ctgctcggtt ccattgccaa
      781 agtagcagcc tcagcccatg cgccgtttat tgccggggcc tctccgtcag tgttgcagat
      841 ggactcctgg caggaactgg ctaacccgcg tgatttgatc aaaatcgtga cccaaaacct
      901 cgaatacgca ccgtggaact cgctgcgaaa aagtgaggat tctcgctaca ttggcctgac
      961 gatgccacgt tttctctccc gtttgcctta tggtgcgaaa acaaaccctg tcgatgagtt
     1021 tgattttgaa gaagatgcgg atggcgcaga tcacagcaaa tatgtctgga gcaatgccgc
     1081 ctatgcgatg ggtgtaaata tcaaccgttc tttcaaacat tatggttggt gtacgttgat
     1141 ccgtggtgtg gaatctggtg gcgcggtgga aaatctgccc tgtcatacct tcccgaccga
     1201 tgacggcggc gtggatatga aatgcccgac agaaattgcc atctctgacc gtcgtgaagc
     1261 tgaactggcg aaaaatggtt ttatcccact tatccaccgc aaaaactctg attacgccgc
     1321 ctttattggt gcgcagtctc tacaaaaacc gcaggagtat tacgatccgg acgctacggc
     1381 caacgccaat ctttcagctc gtttgccata tcttttcgca tgttcacggt ttgcgcactt
     1441 cctcaaatgt atcgtgcgag acaagatcgg ctcgttcaaa gagcgtgaag atatgcagcg
     1501 ctggctcaat gagtggatca tgaactacgt cgatgccgat ccggtgaact cctcacagga
     1561 aaccaaagct cgtcgcccac tggcagctgc tgaagtagtg gtggaagagg tggaag
//
LOCUS       DADLSA010000318         1615 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.324, whole genome shotgun sequence.
ACCESSION   DADLSA010000318 DADLSA010000000
VERSION     DADLSA010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.324"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            66..458
                     /gene="ydeI"
                     /locus_tag="JN691_004320"
     CDS             66..458
                     /gene="ydeI"
                     /locus_tag="JN691_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HAY7245529.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            713..1603
                     /gene="dgcZ"
                     /locus_tag="JN691_004321"
     CDS             713..1603
                     /gene="dgcZ"
                     /locus_tag="JN691_004321"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HAY7245530.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSYMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT          454 a          312 c          387 g          462 t
ORIGIN      
        1 tttggcaatt ataaaaatag caaccagact taataagtca aaccaaacgc aattaaggag
       61 ttagtatgaa atttcagtcc attgtattag caagttttct tgtcatgcct tatgcgttgg
      121 cagatgacca gggcggttta aaacaggatg cagcgccacc gccgcctcat gcaatagaag
      181 atggctatcg cggaaccgat gatgcaaaaa aaatgaccgt tgatttcgca aaaaacatgc
      241 acgatggcgc ttcggtttca ctacgcggta atttgatttc tcacaaagga gaggaccgtt
      301 acgtttttcg cgataagagc ggtgaaatta atgtcgttat tcctgcggcc gtctttgatg
      361 gacgagaagt tcagccggac cagatgatca acattagcgg cagtctggat aagaaaagcg
      421 cgccggccgt cgttcgggtc actcatttgc agaaataaaa ataaattagc ctgatggcct
      481 gaactactgg gccattaggg tattgggtta tgctatttgt catcctttta tttacgataa
      541 tgcagaggtg atttacagaa ttaaacactt ttttatccgc tgattttgtt ttaaatcaca
      601 atgttatctc agagatgact cataacttcg gatttctatg aaaatattga cgtaattttg
      661 cttttctctc gttagaatag cgcgcacaag gaactgtgaa aaaggagtgg caatgatcaa
      721 gaagacaacg gaaattgatg ccatcttgtt aaatctcaat aaggctatcg atgcccacta
      781 ccagtggctg gtgagtatgt ttcacagcgt ggtcgcgaga gatgccagta agccagaaat
      841 aacggataac cattcttatg gactgtgcca gtttggtcgg tggattgatc atctggggcc
      901 actcgataac gatgaattac cttacgttcg gctaatggat tctgcccatc aacatatgca
      961 taactgtggt cgggaattaa tgctggctat tgttgaaaat cactggcagg acgcgcattt
     1021 cgacgccttt caggaggggt tgctttcttt tactgcggca ttaaccgatt acaaaattta
     1081 tttgctgacg atccgtagct atatggatgt tttgacggga ttgccggggc gtcgggttct
     1141 tgatgaatcc tttgatcatc agttacgcaa cactgatcct ctgaatcttt atttaatgtt
     1201 gttggatatt gaccgattta aattggttaa tgatacctac gggcatttaa tcggcgatgt
     1261 agtattacgc accctggcaa cttacttagc cagttggacg cgtgattacg aaacggttta
     1321 tcgctacggg ggcgaagaat ttatcattat tgttaaagcg gctaatgatg aagaagcatg
     1381 tcgtgcaggt gtcagaattt gccagttagt cgataaccat gccatcacac attctgaagg
     1441 gcatatcaac attaccgtga cagcaggtgt gagtcgcgca tttcctgaag agcctctgga
     1501 tgtggtcatt ggaagagcag accgggcaat gtatgagggt aagcaaaccg gaagaaatcg
     1561 ctgcatgttt attgacgaac aaaatgtgat taaccgagtt taagctccgt ttaac
//
LOCUS       DADLSA010000319         1609 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.325, whole genome shotgun sequence.
ACCESSION   DADLSA010000319 DADLSA010000000
VERSION     DADLSA010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.325"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(250..1609)
                     /locus_tag="JN691_004322"
                     /pseudo
     CDS             complement(250..1609)
                     /locus_tag="JN691_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
BASE COUNT          572 a          229 c          282 g          526 t
ORIGIN      
        1 tatgtaatta ggcggttaaa ctttaatatt cgcgttaagt ttgggtaaac gcttgtaaat
       61 ggtatcgcta attctttaat tgactaatat ttgaaacggt tagtgtgatt gtatataaat
      121 atttgagtgt aaggtattga aaggggcatt cagcccctcc gtctatttgt gattgcatcc
      181 attgatgcgc gtattatttt gagcttgaaa aagggaggcg ctctttaata ataaataaat
      241 ctatcaaagc taaatattaa atggaagcca cccttttttc tctgatagtt ttattatcac
      301 caaaccattt atttcagtta tcgaatctgg attctgccag gaaggtaaac tcttcacaat
      361 tggataaatc ttctctattt gttctttatt tgctggtttg caattacaga ttccatagta
      421 attcataaat gcaaccatcc taaaatagtt gccaccatcc caccaaaaaa agattttcca
      481 aaagtgtctg cgctcatgga gagcatattt cctgagtcgt ttgaaccatg tatatatatg
      541 tattttccgt taagggaaat gccttttgct tgagatatat atgatatttc tttagctaaa
      601 gagatatctt tctgtcttgc aagagtatca ttcaaaaata gatttttgga tattgaaacg
      661 ctattaaata taataaataa aaaaacatgt agtttaggtg ttttatcgag tgaaaattgc
      721 ttaagcaaga aaacaaaatt actgctacta ttggcattaa aacaaacagg cgaggtagcg
      781 cccctgagcc aacaacaacg ataaatataa ccggaagtat ataaataaaa atgattgatg
      841 aaatcaaata aattgatttt gtttttaatt tatatattag ggtgaatatc agaactaata
      901 aaggaatcag taaccacttg tataagttta aaccagtagg aggattgtta tagaagttat
      961 atatattatc tatggctgat ttaacaagcc atttaatatc gcttgcattt tgtatgtaat
     1021 ttgagatgaa ctcgttcgat tcaagcgaaa aataatgctt gatagctttg gttaatagca
     1081 ggtaaattaa ggtggataaa gctatgaggg atagagataa acatgaaata taaaaagact
     1141 cacgaatatt tttctctttt cgtattatcg aatttacctg caacccaatg acgaatgcaa
     1201 taatatatgt tacgaatgtt tggtagatta ccattgaaag aatggacagt actataccag
     1261 aaaatatcac aatcctattt ttttgcgagt gaaaaataat tgctgatatc gctgccagta
     1321 gaaaagcaat tcccaccgta tcagcttggt taagaaactc taattgatag gatatctgag
     1381 gaaaggtaat aaatactaac atacctatca ataattcata agattctagc tttagcgatc
     1441 tgcagattat aaatgctgaa gtgatgataa aaaataaagc tactaatggt gttatataaa
     1501 gtgaaaaagg ctcagggaga aaataatgtc gcaaaaaagt gtgaaaccac cgtccaagcg
     1561 tgattgtttg ataaaaatta tttgtaaact cgccatcaat attaagtgt
//
LOCUS       DADLSA010000320         1599 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.326, whole genome shotgun sequence.
ACCESSION   DADLSA010000320 DADLSA010000000
VERSION     DADLSA010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.326"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..399)
                     /locus_tag="JN691_004323"
     CDS             complement(<1..399)
                     /locus_tag="JN691_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HAY7245531.1"
                     /translation="MSRLVVVSNRIAPPDEHAGSAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYH"
     gene            complement(374..1174)
                     /gene="otsB"
                     /locus_tag="JN691_004324"
     CDS             complement(374..1174)
                     /gene="otsB"
                     /locus_tag="JN691_004324"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HAY7245532.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(1341..>1599)
                     /gene="araH"
                     /locus_tag="JN691_004325"
     CDS             complement(1341..>1599)
                     /gene="araH"
                     /locus_tag="JN691_004325"
                     /EC_number="7.5.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Part of the ABC transporter complex AraFGH involved
                     in the high affinity transport of arabinose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HAY7245533.1"
                     /translation="FSGQPMTSIGYELIVISACVLGGVSLKGGIGKISYVVAGILILG
                     TVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
BASE COUNT          389 a          430 c          378 g          402 t
ORIGIN      
        1 gtgataatcg tggatccaga taatgtcatc gtcttgcaac agcggcagta atttatctgc
       61 cagcaacgca tttacgcgta gatagccgtc ccaggcagga cgctgaaatt gcaccagatc
      121 gagccgataa tgaaaagcgg gccagagaac ggcattggag aattggttgt agtattcgtc
      181 aaggtcctgt tcgctgaggt taaaagaggc ccacgtaatg ttaccttttt tcaccttttt
      241 tagcggctga tcctcattcc ctgtttcacc actccagcca aaccacagtc cgcctgcggc
      301 tttcagtgcc cccagtatgc caacggcaag gccaccggca ctgccggcgt gctcgtctgg
      361 tggtgcaatc cggttagata ctacgactaa acgactcata gtcatcactc ctgttatttt
      421 ctcttttttg ttgtaatgcg gtggttatca tttcaagcca gctccagaca tccggcacac
      481 ccgccagtcg ccatgatgcc tgagttgcac ctgtgccaat ttttactgac attccgccca
      541 gtcggttaac gactgcgaag ccagattcat cggttaaatc atcgcccaga aatacgggcg
      601 ttcgcccgat aaaaggagct tcctgcataa aggctgcaat cgcttcacct ttactggtac
      661 ctctcggttt gatctcgaca acacactttc cctgctgtaa cgccatttgt ggccagatct
      721 gagtaatacg ttgcgctaat gtcattaatg cgtcttcatg ctgcggagcc tgacgataat
      781 gcagcgcaaa agccatccct ttcgcctcca gctccgcgcc gggatactga gcgatgaccg
      841 tatgcagttg cacgctaata tcacgcgcaa tcgcatccgg cagatgaacg atatgtgttt
      901 taccattgat gtcacggcgc tccgccccat gcacgcccgc taacgggaag cgataaggtt
      961 ttgccagtgc gtcaagctcc accattgagc gccctgatat caatgccaat gcaccatcac
     1021 ttgcggttgc cagtagctgt agtccttgca gaatattgtc aggcacgacg acctgatcgg
     1081 gatgcggttt gatttccgcc agcgttccat caagatcaaa aaaccaggca tatttcgcgg
     1141 atagttcagg ggtttcggtt aacggttctg tcacccggct ctcctccttc tcttttctta
     1201 ttgtgttgcg cagaccgcgc tttacagaca tgtaagtata gacagtgtga cggaggtcgc
     1261 catttgcaac gaagaacagc caaccgttgt ggcggctggc tgtggtggga aaaaacgtta
     1321 aattgttgtg gaaaaaagca tcagacagtg cgtttcgctt tttgcttgta acggtcgaag
     1381 atcaccgctg ccagcaggat taagccgcga accacgtact gcgcgaaagg agaaatatta
     1441 agcaggttca tggcgttttc cacggtgcct aaaattaaga tacccgccac cacatatgag
     1501 atttttccga tgccaccttt cagagaaacg ccacctaaaa cgcaggcgga gataacaata
     1561 agctcataac caatcgacgt cattggctgc ccactaaat
//
LOCUS       DADLSA010000321         1598 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.327, whole genome shotgun sequence.
ACCESSION   DADLSA010000321 DADLSA010000000
VERSION     DADLSA010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.327"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..388
                     /locus_tag="JN691_004326"
     CDS             <1..388
                     /locus_tag="JN691_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245534.1"
                     /translation="LVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(389..1297)
                     /locus_tag="JN691_004327"
     CDS             complement(389..1297)
                     /locus_tag="JN691_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7245535.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLIDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERPGFQVYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            1397..>1598
                     /locus_tag="JN691_004328"
     CDS             1397..>1598
                     /locus_tag="JN691_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HAY7245536.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYS"
BASE COUNT          423 a          356 c          381 g          438 t
ORIGIN      
        1 cctggtgctg gtggcaattc tgattatgct gccgactctc ggcatcggtg atatgaacaa
       61 tctctataaa tggctgttga atcttaactc ggtagtgatg ccgctgcgtt atttatgggt
      121 atttgttgca tttattgcag tcgttcgcct ggcgcagaaa tataaaccag aatatgtctt
      181 tattcgtaat aagcctctgg caatgaccgt cgggatttgg tgttttgcct ttaccgcctg
      241 tgcctgtttg acggggatct tcccgaaaat ggaagccttc actgcagagt ggaccttcca
      301 gttggcgctg aatgttgcaa cgccgtttgt gctggtagga ttaggactga tattcccgct
      361 gctggcgcgt aaagcgaata gcaaataatt attgtggtcg gtgacgctga ctctggcaga
      421 tctgacccgt ttttacataa tcgagaaatg cgcgcaagcc gcaggacata tactggcggt
      481 ttgggtaata gacttggaag cctggacgct ctgtgatcca gtcttccagt acacattcta
      541 aacgaccagt atcaaggtac tccttgatct gttcatagag cagatacccg ataccagcac
      601 ccatcaaaac ggcctccagt tcagcatcaa catcatccag aatgatattt cctgcgacag
      661 caatttccag ctcctttgca aattgccagt ggaaaggttt gccgctggga taacgaaaaa
      721 caacacattg atgattaagc agatcatgag gatggcgggg tttgccatat cttgcaaagt
      781 actctggcgt tgctgctaca cataatttca ctggtgggcc gatcgccacg ctgatcatat
      841 ctttttcgac gatgccacta agtcgaacgc cagcgtcaaa gccctgttgg acgatatcga
      901 taagagaatc gtcagttgtg agctctactt taatatcagg atattcacgt gtgaaaccaa
      961 ccagtaaagg cattaaaaat atacgtgcag ctacccgggc ggcattgatt ttgagcgttc
     1021 ctgtcggtgt cagacgaaaa tcattcatct catctaacat gatctgaatt tcgtcgaaag
     1081 ctgggcgcaa tcgttcatag agattagaac ccgcttccgt taacgatacg cttcgggttg
     1141 tccgattgaa taagcgaatt ttaagacgtt gttccagtgt tttaatacta tggctaatgg
     1201 cggacgagga taagcctaac tcatcgcctg ctgcacgaaa gctttgatta cgggccacgg
     1261 caaagaaagt ggcaaagtca gacatattca tccgcattag tgaaaatcct tcattaactc
     1321 atcctgaatc atcacgttta caggattatg ccgcctgcgt aaagtgcgct ccagaactta
     1381 acgtggaggt aaaattatgc agtctgaacg tatttatttg gtatgggccc atcctcgtca
     1441 tgattcattg accgcaaata ttgctgatgc gatccatcag cgggcaatgg agcggaaaat
     1501 acaggtgacg gaactcgatt tatatcggcg taatttcaac ccggtgatga cgtcggaaga
     1561 tgaaccagac tggaagaata tggataaacg ttattctc
//
LOCUS       DADLSA010000322         1585 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.328, whole genome shotgun sequence.
ACCESSION   DADLSA010000322 DADLSA010000000
VERSION     DADLSA010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.328"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..205)
                     /locus_tag="JN691_004329"
     CDS             complement(<1..205)
                     /locus_tag="JN691_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAY7245537.1"
                     /translation="MDDIVNSVPSWTFTAIIAVCVLFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEII"
     gene            complement(232..861)
                     /locus_tag="JN691_004330"
     CDS             complement(232..861)
                     /locus_tag="JN691_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiJ family protein"
                     /protein_id="HAY7245538.1"
                     /translation="MNLFADHNTPYLFAISFVLLIGLLEIFALICSHMLSGTLDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLQEPEEGKIFTKGDKVLIICRLSATRYLVENNPWPQIL"
     gene            1130..1330
                     /gene="glgS"
                     /locus_tag="JN691_004331"
     CDS             1130..1330
                     /gene="glgS"
                     /locus_tag="JN691_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HAY7245539.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(1373..>1585)
                     /locus_tag="JN691_004332"
     CDS             complement(1373..>1585)
                     /locus_tag="JN691_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HAY7245540.1"
                     /translation="LDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRA
                     ELSANPAETVVEGPFSKDVILKITYN"
BASE COUNT          487 a          303 c          354 g          441 t
ORIGIN      
        1 ggattatttc atgaaagatc ggcatcacga ttgcgccacc gctcatcacc actttttgcc
       61 cacctaaacc agtacgaaca aatgcttgct ctgccgaagc gcgacgatag agcctggcga
      121 aaataattcc aataataaac agaacgcata cggcaataat tgcggtaaac gtccaggaag
      181 gcacagaatt aacgatatca tccattatta aatttccttt tgattaataa attacaatat
      241 ttggggccag ggattgtttt ccaccaaata tcgagttgca gaaagtcgac aaataattaa
      301 cactttgtct cctttagtga aaatttttcc ttcttcaggc tcctgtagta aataatgtat
      361 ttggccaaat tgatcggtga gttttccttc gcagggatta cctgaagtgg cttgatgacc
      421 ggtaattaac gccatgctac caatatattc ttcttctgta atagctgaac tatggtcgcg
      481 tggaatccag ggcgcaacta ttttaccggt ataatgcacc gcaataatcg taaacagtaa
      541 actgacagga accacgaaca ggttcgacag tggtgattgc cagaccatga tgcaggcatg
      601 ttgcaacagg atgccaataa gaccaaaaaa accagcgagt aagcagagaa cgacgagagc
      661 tggcaatctt ccgatattga gataatgaag tgcctggcta atatgacctg tggttatgga
      721 atcataatgg tcaagatgtg catcaagagt accggagagc atgtggctac agataagcgc
      781 aaaaatttct aaaagaccga tcaacagtac aaaggaaata gcgaaaagat aaggggtgtt
      841 atggtcggcg aataaattca tatgtcactc caatgtccat ttagtgacgc aatatttacc
      901 ttggaatagt ttccctgctt acatttgtct ttgttaataa taacacatgt tagagaagtt
      961 ctggaagaga aagtatttta atatggtttt ttatttttca ctaaaaagtg tgatcgggga
     1021 caatatattt acgcacgtta tgtttaaagg cactacactg attggggaat actgaaatca
     1081 gaaaaagcat atggccttaa aaggtatgcg ataaccggag gatgtgctta tggatcatag
     1141 tcttaattct ttgaataatt tcgatttcct ggcgcgtagt tttgccagaa tgcacgcaga
     1201 aggtcgcccg gtcgatattc tggccgttac tggtaacatg gatgaagaac atagaacctg
     1261 gttttgcgca cgttatgcct ggtattgcca acagatgatg caggcaagag agctggagtt
     1321 agagcactga tataacgggc ctgatggccc gttttagtgt ttgtattaga tgctagttat
     1381 aggtgatttt gaggattacg tctttactaa acggtccctc aacaacagtc tctgccgggt
     1441 tcgcactaag ttctgccctg aatggtgcgg tgaccgaagg tgcactagac gatgaaaaag
     1501 aagcaaattg tgagaagcgg ttaaattccg ttgctgttcc atctttatga aatatcttca
     1561 atagcagtcc attacccata tccag
//
LOCUS       DADLSA010000323         1577 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.329, whole genome shotgun sequence.
ACCESSION   DADLSA010000323 DADLSA010000000
VERSION     DADLSA010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.329"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..174)
                     /locus_tag="JN691_004333"
     CDS             complement(<1..174)
                     /locus_tag="JN691_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY7245541.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYP"
     gene            173..1312
                     /gene="queG"
                     /locus_tag="JN691_004334"
     CDS             173..1312
                     /gene="queG"
                     /locus_tag="JN691_004334"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAY7245542.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(1295..1348)
                     /locus_tag="JN691_004335"
     CDS             complement(1295..1348)
                     /locus_tag="JN691_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245543.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT          407 a          388 c          406 g          376 t
ORIGIN      
        1 aggatacgcc gaacgacaca cctggaatgc ggcctcgcca gcgcgaagca tcagctcata
       61 gagtgtgagc cccagcacat ctgccgcctc gcgttctccg cggcggatat cgtcggcgta
      121 ccagacggtg tgtggtatac ttacggggtt tttcttcatt gtatggtccg tcatgtcaga
      181 gcccctcgat ctcaatcagt tagcgcaaaa aattaaacag tgggggctgg aactgggctt
      241 tcagcaggta ggtattaccg ataccgatct cagcgagtcc gagcccaaac tgcaagcatg
      301 gctggacaaa caataccacg gcgaaatgga ctggatggca cgtcacggta tgctgcgcgc
      361 tcgcccccat gagttattac ccggtacgct gcgtgtaatc agcgtgcgga tgaattacct
      421 tcctgctaac gccgcatttg ccagcacgct gaaaaacccc aaactcggct atgttagccg
      481 ttatgcgctg ggccgtgact atcacaaact tctgcgcaac cgactcaaaa agctgggcga
      541 gatgattcag caacattgtg tttcgctgaa ttttagaccg tttgtcgatt ctgcgcctat
      601 tctcgagcgc ccgttagctg aaaaagctgg gctcggctgg acaggtaagc actcacttat
      661 cctcaatcgc gaggccggtt cgttcttctt tttaggtgaa ttgctggtcg atattccgct
      721 gcccgtggat caaccagtcg aggaaggatg cggcaaatgc gtggcctgta tgacgatttg
      781 cccgaccggt gccatcgtcg agccatatac cgtcgatgct cgccgctgta tctcttatct
      841 caccatcgaa cttgaagggg cgatcccgga agagttgcga ccgttaatgg gaaaccgtat
      901 ttacggttgc gatgactgcc agcttatctg cccgtggaat cgctattcgc aactcactac
      961 agaagacgat ttcagcccgc gtaagccgct acacgcaccg gaactcattg agttatttgc
     1021 ctggagcgaa gagaagtttt taaaagtcac ggaaggatcg gcgattcgcc gtataggtca
     1081 cctgcgctgg ctgcgtaata tcgccgtggc attaggcaat gccccttggg atgaaacgat
     1141 tttgacagcg ctggaaagtc gtaaaggtga gcacccactt ctcgatgagc acatagcgtg
     1201 ggcgattgcg cagcaaatag agagacgaaa tgcgtgcata gtcgaagtgc aattgccgaa
     1261 aaaacagcgt ctggttcggg tgattgaaaa agggttaccg cgtgacgcct gattcatcca
     1321 cagcttgtga ataaaaataa aaacgcattg caattcaaga cgaataaaat cgtcaagtga
     1381 tcgactcgac aaaacggaat cttgattttt aacttattta tcaataggtt aaaaaatacc
     1441 attcaccgtg cgaagtttct ttgaaattaa gattacagcc tgaggctgtg gataactctg
     1501 tttacaagag tttgtcagag acaacaaaaa ccagcaataa cgcatcgcgt cgctgtggat
     1561 attttattga gagaaga
//
LOCUS       DADLSA010000324         1547 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.330, whole genome shotgun sequence.
ACCESSION   DADLSA010000324 DADLSA010000000
VERSION     DADLSA010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.330"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            1..1547
                     /gene="yjcS"
                     /locus_tag="JN691_004336"
                     /pseudo
     CDS             1..1547
                     /gene="yjcS"
                     /locus_tag="JN691_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
BASE COUNT          394 a          423 c          408 g          322 t
ORIGIN      
        1 tgcgcgatgc taatggcaaa ccatattatc gcggggaaga ttataaattt gatatcaatg
       61 cgcccgcgcc cgaaaccgtt aaccctagcc tgtggcgtca gtcgcagtta aacggtattt
      121 ccggcctgtt taaagtcacc gacagaatgt actaggttcg cggtcaggat atctccaaca
      181 tcacctttat cgaaggtgat accgggatta ttgtcatcga cccgctggtg acgccaccga
      241 gcgcaaaagc cgcccttgac ctttacttcc aaaatcgccc gcaaaaacag attgttgcgg
      301 ttatttatac ccacagccat accgaccact atggcggcgt gaaaggcatt atctctgaag
      361 ccgatgttaa atcaggcaaa gttcaggtga ttgccccagc aggctttatg gacgaagcca
      421 tcagcgaaaa cgtgctggcg gggaacatca tgagccgccg tgcgctctac tcttacggtc
      481 tgttactgcc gcacaacgcg caaggcaacg tgggtaatgg ccttggcgtg acgctggcaa
      541 cgggcgaccc gagcattatt gcaccgacta aaactatcgt cagaactggc gagaagatga
      601 ttatcgacgg cctggagttt gacttcctga tgaccccagg tagcgaagca ccagccgaaa
      661 tgcacttcta tattccggcc ctgaaagcgc tgtgtaccgc cgagaacgcc acgcataccc
      721 tgcacaactt ctacactctg cgcggcgcga aaacccgcga caccagcaag tggaccgagt
      781 atctgaacga aacgctggat atgtggggta acgacgcgga agtcctgttt atgccgcaca
      841 cctggccggt ctggggcaat aagcatatca atgattatat tggtaaatat cgcgatacta
      901 tcaagtacat tcacgaccag accctgcacc tggcgaacca gggctacacc atgaatgaaa
      961 tcggcgacat gattaaactg ccgcctgcac ttgccaataa ctgggccagc cgtggctatt
     1021 acggttctgt cagccacaac gcccgcgcgg tgtataactt ctatcttggc tattacgacg
     1081 gtaacccggc taacctgcat ccgtatggtc aggtggagat gggtaaacgt tacgtgcagg
     1141 tgctgggcgg ttccgcacgc gttattaacc tggcgcaaga agcgaacaag caaggtgatt
     1201 accgctggtc ggcagaactg ctgaaacagg tgattgccgc caacccgggt gaccaggtcg
     1261 cgaagaatct gcaagcgaat aactttgaac agctgggcta tcaggccgag tccgccacat
     1321 ggcgcggttt ctacctgacc ggcgcgaaag aactgcgcga aggggtgcat aagttcagcc
     1381 acggcaccac cggttccccg gacaccattc gcgggatgtc ggtcgaaatg ctgttcgact
     1441 ttatggccgt tcgcctcgat agcgcgaaag ctgcgggtaa aaatatcagc ctgaacttca
     1501 atatgagcaa cggcgataac ctcaacctga cgctgaacga tagcgtg
//
LOCUS       DADLSA010000325         1535 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.331, whole genome shotgun sequence.
ACCESSION   DADLSA010000325 DADLSA010000000
VERSION     DADLSA010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.331"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            147..956
                     /locus_tag="JN691_004337"
     CDS             147..956
                     /locus_tag="JN691_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAY7245544.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVGPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLMVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            964..>1535
                     /locus_tag="JN691_004338"
     CDS             964..>1535
                     /locus_tag="JN691_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY7245545.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRHEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNLLRDQFTERLEAMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLP"
BASE COUNT          349 a          373 c          461 g          352 t
ORIGIN      
        1 cgcggggggg tgggaagacg acagtcaggt gaaaagaatg ctggtggagt ggggaccggt
       61 tatcccggaa gggaaggtcg agatcactat cagtaagtac gagaaaacgg cgggtgcagc
      121 tgcctgatta agaggagaaa cgaagtatga ataatctgat ggtcattgat ggtattgaag
      181 ttcgtcgtga tgcttatggg cgttacagcc tgaacgatct gcatcgcgca gcagtagcat
      241 ctggtgcaaa tgccagaacc aaggagccag gaaagtttct ttccagccaa caaactgttg
      301 agcttgttca tgaattaacc aacacccaga atttgggtgt tggcccggtg agtgtgattc
      361 atgggggaaa tgaacgggga acgtatgtct gtaaggaact ggtgtatgcc tatgcaatgt
      421 ggatcagccc gtcattccat ctgatggtga tccgtacttt cgatatggta accagcgcgc
      481 cggaaaagtt atccggacag gctgctgaca agatgcaggc tggcgtgatt ctgctggact
      541 ttatgcgccg ggagttaaat ctgtctaact catctgtgct tggggcctgt cagaaactcc
      601 aggaggctgt tggcttaccg aatctggcac cgcgctatgc cattgatgca cctgctgacg
      661 cgcctgatgg ctcaagccgc ccgacgctgt cgctgagtgc actgctgaag cagtatggta
      721 tccgcctgac ggctaatcag gcatatcacc agatggcgaa gctggggatc gttgaacaac
      781 gcgaacgata cagccgtacc gcgattaaca acatcaaaaa attctggtcg ctgacagcga
      841 aaggttgcat gttcggcaag aacatcacca gtcccgcaaa tccgcgcgag acgcagccgc
      901 atttcttcga atcccgattc cctgagctgt taaagctgct cgataccgtt cattgaggtg
      961 accgtgagag cactactgac ccctgaaatt gccccgcgta tggggatcgt attgttcagg
     1021 ccaggttcag agctgatgcc cctgtttatg caggggcgtg tcctgctgga gcctgagccg
     1081 gaacgttatt catctttcgc cagcggtgcc gttccggcag catcacaacc gctggcggat
     1141 gatcctgccg ttcgggccgt gttccgccat gaggcggtga tccgtcgtgc tggtggcgtg
     1201 gaatgccttg agagctggtt acttcgtgaa aagggctgtc agtggcctca ttccgactgg
     1261 cacagcgaga acatgaccac aatgcggcac gcgccaggcg caatccgtct gtgctggcac
     1321 tgtgacaatc ttctccgtga ccagttcacg gaacggctgg aagcaatggc aacggataac
     1381 tgtgcccgct gggtgttgtc tgttgtgcgc cgtgatcttg gttttgatga cagtcacgtt
     1441 gtgacaatgc cggaactgtg ctggtggctg attcgtaatg acctggcgga tgccttaccg
     1501 gaaagtgcag cccgtaaggc actgagatta ccgaa
//
LOCUS       DADLSA010000326         1510 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.332, whole genome shotgun sequence.
ACCESSION   DADLSA010000326 DADLSA010000000
VERSION     DADLSA010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.332"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(94..243)
                     /locus_tag="JN691_004339"
                     /pseudo
     CDS             complement(94..243)
                     /locus_tag="JN691_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            606..1355
                     /locus_tag="JN691_004340"
     CDS             606..1355
                     /locus_tag="JN691_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY7245546.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          416 a          311 c          326 g          457 t
ORIGIN      
        1 cgctgggtta tcgctcgcca cgggaatatc tgcggcagcg ggcttgtaat gggttaagtg
       61 ataacagatg tctggaaata taggggcaaa tccagatgtt ttcagaccaa actgctcctg
      121 tatgacccgg taatcgtatg cgcagtactg tgaacctcga tcggagtggt ggattagccc
      181 ggcaggtggg cgctggctcc tgagcaccat aaacagggct ttacccgtca atattggctc
      241 cttctgatgc cattctattt caggaaggag tgtccgttaa actcaggcta cctcaaaaag
      301 ttatcggtgc gtttatcgaa aaacatatgt tctactaatt ggagtcattg tctgtaaaag
      361 caactcaaaa atacaagttg cttattcccc ttgttggtaa gaacttaatt ctaactgttt
      421 gttctgggtg ttttaaacgt cctccttttt atgcttgttc ataacggata tttaataggt
      481 aacctttgtt ttagcatctt ctgatttatt taaattatgt gatagcgcac acaattttat
      541 tatccagtac tttatactct taataaaata attataaata tttatcatct ataaggagat
      601 gttatatgaa aagacagctt tttactctta gtattgtcgg ggtattttct ttaaatacct
      661 ttgcatcatt ccctccggga aatgatgtga caacaaaacc tgacctttac tacctgacaa
      721 acgataatgc tattgacagt ctggcattat taccgcctcc accacaaatc ggaagtattg
      781 ccttcctgaa tgaccaggcc atgtatgaaa aggggcgttt attgcgaaat actgaaagag
      841 ggaagctggc agctgaggat gccaatctca gtagtggtgg cgtggcaaat gtattttctg
      901 ctgcttttgg ttctccgata acggctaaag attcaccaga attgcataaa ttactgacaa
      961 atatgattga ggatgccgga gacctggcaa cgcgttcagc aaaagaatat tatatgcgta
     1021 ttcgaccttt tgctttttat ggtgtttcta cgtgtaatac aaaagaacag gatacattat
     1081 ccagaaatgg ctcttatcca tcaggtcata catcgattgg ttgggcaaca gcacttgtat
     1141 tgtccgaaat aaatccggca cgccaggata ccattctgaa acggggttat gaactggggg
     1201 acagcagggt tatttgcggt tatcactggc aaagtgatgt tgacgcagca cgtattgttg
     1261 gctctgctat tgtagcaaca ctgcactcaa atcctgtgtt ccaggcacaa ttacaaaaag
     1321 cgaaagatga attcgctaac aatcagaaaa aataatgctt ttagccagta cgccttataa
     1381 ccccgacctt cagggctaat gccagtcagt taagcaactg actggctctt tttcagggct
     1441 gtggggtatt tccagggcct ctcctttacc actctcggga aggccctttc ccttcttgtc
     1501 ggtaatttca
//
LOCUS       DADLSA010000327         1507 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.333, whole genome shotgun sequence.
ACCESSION   DADLSA010000327 DADLSA010000000
VERSION     DADLSA010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.333"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..1008
                     /locus_tag="JN691_004341"
     CDS             <1..1008
                     /locus_tag="JN691_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245547.1"
                     /translation="VGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAAR
                     KVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQ
                     SVEYVPLACEAHSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEM
                     IKNRMAELFNQSDISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETL
                     EGDTNVAVVMGCKSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSL
                     TPEELMMLKDLFDIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMG
                     V"
BASE COUNT          461 a          282 c          342 g          422 t
ORIGIN      
        1 gtaggtccag atatacttgc attgcccgtc aacgtatatc atgcggattt taaaattaaa
       61 atcaataatg cagtggagaa ggcttcatca attgtgttat gtgctgtgca aaaaaatctc
      121 catgcagcaa ggaaggttct tagcgggata ataggaaatg tgccaaaaaa taataaacca
      181 aaaacaatta tttttgattt gcgttttctt agtgcaatta agcagaaagt ttttttgggc
      241 agtgaaaaac cgttgtttgt aaagcatacc gcaatgatga gggaggcatg taaggaatta
      301 ccgcagtcag tggaatatgt tcctttagcc tgtgaagcgc atagccatcg cagcgtcgcc
      361 cttgccaaaa ccgtggctgc gcccatcatg gcgacaggtg tgggcttggc attaatgtat
      421 gcaacatcat caggaccgta ttatgcgcaa agtctttctg gttcgccgga tatcgagatg
      481 ataaaaaatc gcatggctga attgtttaac cagtcggata tttctgtgcg caacaaatat
      541 agcccggctt ttgataaatg gaatgaactg gtagataaat taaatcacga aagaaataca
      601 gttccgtttg cgtgtttgac caccatttta gcgacggcga taaatgaaac tcttgagggg
      661 gatacgaatg ttgccgttgt gatggggtgt aaatctgcga aagatcgcac gatatccatt
      721 gtccttggca actcgatgtt gcaaacactc cttgaaaaac gcctcgccga tagtggagaa
      781 atagaaaatc tttttgatca acagggatat tttagttgcg attcattaac acccgaagaa
      841 ttaatgatgt taaaagacct ttttgatatt cgagttcttc atgtctctaa caaattcaat
      901 gttggattgc aaggtaatat caacactgat gttttacaag attcgttttt taaaaatgtc
      961 gatttcattc atgaatataa ctcgtatacc gtagggatgg gagtctgacg agtaaaactc
     1021 attaggatta tcagaattcg gacaatgcat tgcaaaaatg acatacaaag agagatgtgt
     1081 gcaggtactt agccgcgagg ggcatggcga agccatacag aaagtaccag tattcagaac
     1141 tccagtgaaa gcggcatagt ggccgcctgc tcaggagttt tgctatgacc cgtaatcgtg
     1201 ctgaacgtcg ccaccctatg atgcgattaa aaaaactcat cgtcgcgctg atgattgacg
     1261 tcaatgctat cggatgatgc cgggggtgtt ccggaaggaa ttccttgttt cccgaactgt
     1321 taccaacaaa attataacga tgagcaagta aagaccggtt ggtggcagta ctgaccggtc
     1381 tgtgatgaaa gattaatttc accttaaatc ctggtgctgt taggatgctg acgtttttcg
     1441 gctacaccat tcagattatg caaataaata tccctgaata taatatattc atagcaccca
     1501 taactca
//
LOCUS       DADLSA010000328         1505 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.334, whole genome shotgun sequence.
ACCESSION   DADLSA010000328 DADLSA010000000
VERSION     DADLSA010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.334"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(877..1353)
                     /locus_tag="JN691_004342"
     CDS             complement(877..1353)
                     /locus_tag="JN691_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7245548.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          352 a          398 c          376 g          379 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcagcgtaa aagatctatt ctaggcaagg gtgttgcgtt
       61 gcacgcaaca ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgccagc cgtgattttt gccatccgtt
      181 caccgttaaa acgcctcaga aatcgggaca ggatgtgtaa tcgtataacc gcgcatgcac
      241 gccgatacga ttaccacttg gtcagggctt caccccgaca ccccgcatca gtgcgtaagt
      301 gctcaatgtg gcttccggtt ctcttccgct tcctcgtcga ttcgctcgct acgctcggtc
      361 atcgacttgc ggcgagcggg atcacctctc gcatgttgtc ggtgatcccg gtgttgttct
      421 ttcggggatg agtcgccaga tagcccctca aacgcgctca aaagctctca gacgcacgaa
      481 acgggtttta gatgcccgcc agggcatgga aggcgcgaaa aacgcttaca gaggctctca
      541 ggaggtcagg cgcgccgata cccctaacca ggggctttca ggtcgccagg aaccgcgcca
      601 gccccctgca aagcccattt gcacggaaca ggcagccatg cggttggtag gtggtcgtag
      661 accgacggtg ttgcagcgat gggggtctgg ggttctcccc agacggtttc cggtagcgct
      721 caaggcgctg ccggaaaccg tggcggcgcg gttttttctg gagaacgggg agcaaaaaca
      781 cgcaaaggat tttgaggcgg caaaagagca atgcaggata gacaatcatg gtcagaacgt
      841 taacacgtag cccctgattg tccgcctgcc gctagctcaa tttgaaaaca tggccatgat
      901 tcggcgctga atatcaccgt ctttgttgac cagtgtttta gtgacaaacg ctgcatggtt
      961 tttcgtagtg ttgtatccct tccagacttc atgtggatcg agggcatacg cacaaacctt
     1021 accagcaacg gggattctaa ctaatgcatt ttttgattct aagtaattga gccaccgaat
     1081 aattgtgctt ctgctaactc caagtttttc agctaggaat tgctgatccg caacgacagc
     1141 gccacacgta ggatcgatat gctccgctaa aaatgagtac agcttcagtg ctgatatgcc
     1201 ttggctatcc ttcagcagct ctcgaacgcg ttcccaacct tttggggata cctgtgtaaa
     1261 ccttgaattt ttctttgctt cttcgagatc ctcggtgttt ttctttgcct catctgaaag
     1321 ttgtttcagt cgtttttctg aatcaataaa catgattttt tccctctgaa aatcccatgc
     1381 actaacgttg gttcaaatct aagccatcat gacacatctg tgcaacacct tgttgcgcgt
     1441 tgtcgcatcc tggatgcgac aacgttgtgt caccctggat gcgacaggta aataaaaaac
     1501 acact
//
LOCUS       DADLSA010000329         1478 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.335, whole genome shotgun sequence.
ACCESSION   DADLSA010000329 DADLSA010000000
VERSION     DADLSA010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.335"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            49..1029
                     /locus_tag="JN691_004343"
     CDS             49..1029
                     /locus_tag="JN691_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAY7245549.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWATALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT          406 a          345 c          345 g          382 t
ORIGIN      
        1 taaaggggcc ctgctatcaa tttgtcattt gtattacgcg aaaattatat gaacgcaata
       61 atatcgcccg attattacta tgttcttacc gttgctggtc agtctaatgc catggcgtat
      121 ggcgaaggac tgccattacc ggacagggaa gatgcgcctc attccagaat taaacaatta
      181 gcgagatttg cgcatacgca tccaggaggc ccgccatgtc actttaacga cattattcca
      241 ctgactcact gcccacacga tgttcaggat atgcagggtt atcaccatcc tctggcaacg
      301 aatcatcaaa cacagtacgg caccgttggc caggcactgc atattgcacg gaaattactg
      361 cccttcattc ctgataatgc agggattctt atcgttccgt gttgccgtgg cggatcggct
      421 tttaccgcgg gcagcgaagg gacatattca gaacggcacg gagccagcca tgatgcttgc
      481 cgttggggaa cggatactcc gctgtaccag gatttagtca gcagaacgcg agccgcactg
      541 gcaaaaaatc cgcagaacaa attcctcggc gtatgctgga tgcaaggcga atttgactta
      601 atgaccagtg actacgcgtc acaccctcaa cactttaatc atatggttga agcctttcgt
      661 agggatctaa aacaatacca ttctcagctt aataatatta ctgacgcgcc gtggttttgc
      721 ggcgatacca cctggtactg gaaagaaaat ttcccgcatt cgtatgaagc tatttatggc
      781 aattatcaaa ataatgtttt agccaatatt attttcgtcg acttccagca acaaggtgaa
      841 agaggactga cgaacgcgcc tgatgaagac ccggacgatt taagcacggg atattacggt
      901 tcagcgtacc ggtcaccgga gaactgggcg acggcactgc gaagcagtca tttcagcacg
      961 gcagcccgtc gggggattat ttctgacaga tttgtagaag caattttgca gttttggcgc
     1021 gaaaggtgag cggggagttt attcatgggg gatgtccttg ttcgttctca gagtagacaa
     1081 caaaaaacaa atgagatcaa ggtaatatct cttcacttgc gggctcgaac agcaggcaat
     1141 tgccaatata ataatctggc acaagggtga aaaacgcgca ccgcataaac cgttgttatt
     1201 gcttacagat tagccggatg ccttaataga catccgcgcc ttttcgatta tggctcggaa
     1261 atctacgaac atctacacag tttactggaa cgttttgggc cacagcgttc gcagcaccgt
     1321 cctgatatgc cgttctggcg attataagtc gacggattct ggcaaataga cgatgatgat
     1381 atgcttaatg atgttcaaag aaattaggcc attattatat tattgcttgc attttctttc
     1441 attcctaaaa gcacccgcca ttaatcccag gcgcatta
//
LOCUS       DADLSA010000330         1446 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.336, whole genome shotgun sequence.
ACCESSION   DADLSA010000330 DADLSA010000000
VERSION     DADLSA010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.336"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..369
                     /locus_tag="JN691_004344"
     CDS             <1..369
                     /locus_tag="JN691_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7245550.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLK
                     GKLTPALLQILIREIKGSSH"
     gene            369..716
                     /gene="tnpB"
                     /locus_tag="JN691_004345"
     CDS             369..716
                     /gene="tnpB"
                     /locus_tag="JN691_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7245551.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWLVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            736..>1446
                     /locus_tag="JN691_004346"
     CDS             736..>1446
                     /locus_tag="JN691_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7245552.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQILEAQERISALQEEMAETLGEQYDPVLPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVAGKYADYLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAEL"
BASE COUNT          343 a          403 c          398 g          302 t
ORIGIN      
        1 cctaattttc cttatgaatt taaaatcgcc ttagtggagc agtcactgca gcccggagcc
       61 tgtgtggcgc agatcgcccg tgaaaacgga atcaacgata acctgctctt caactggcgc
      121 catcaatacc ggaaaggtgg cctgctgcct tccggaaaaa atatgcctgc actgcttccc
      181 gtgacgttaa cgccggagcc ggataataaa atcccggctc cggcacagga accagagcag
      241 ataaatacac cgtccgacag tctgtgctgt gagctggttc tgccggccgg aactctcagg
      301 cttaaaggta aactgacgcc ggcgttatta cagatactta tccgcgaaat aaaagggagc
      361 agccactgat gatatctttc cctgcaggtt cgcgtatctg gctggttgca ggtatcaccg
      421 atatgcgaaa tggctttaac ggcctggcct caaaagttca aaacgtcctg aaggatgacc
      481 cgttctccgg gcatctgttc atcttccgcg gacgccgggg tgaccagata aaagtgttgt
      541 gggctgacag tgacggactg tgcctcttta ccagacgcct ggagcggggc cgcttcgtct
      601 ggctggtcac ccgtgacggc aaggtgcacc ttactccggc ccagttatcc atgcttcttg
      661 agggcatcga ctggaaacac ccgaaacgaa cggaacgcgc tggcatccgc atataacccg
      721 ttgtaaagtg aggatatgga cacctcactt gctcatgaga atgcccgcct gcgggcactg
      781 ttgcagacgc aacaggacac catccgccag atggcgaaat acaaccgcct gctctcacag
      841 cgggtggcgg cttatgcttc cgaaatcaac cggctgaagg cgctggttgc gaaactgcag
      901 cgcatgcagt tcggtaaaag ctcagaaaaa cttcgcgcaa aaaccgaacg gcagatactg
      961 gaagcacagg agcgaatcag cgcacttcag gaagaaatgg cggaaacgct gggtgagcaa
     1021 tatgacccgg tactgccatc tcccctgcgc cagtcttcag cccgtaaacc gttacctgcc
     1081 tcacttcccc gtgaaacccg ggtcatccgt ccggaagagg aatgctgccc ggcctgtggt
     1141 ggcgaactca gttctctggg atgtgatgtg tcagagcagc tggagcttat cagcagcgcc
     1201 tttaaggtta tcgaaacaca acgtccgaaa ctggcctgtt gccggtgtga ccatatcgtg
     1261 caggcaccgg tgccttcaaa acccattgca cgcagttatg ccggagcggg gcttctggcc
     1321 catgttgtcg ctggaaaata tgcagactat ctgccgttat accgccagtc agaaatatac
     1381 cgtcgtcagg gcgtggagct gagccgtgcc acactggggc gctggacagg tgctgttgct
     1441 gaactg
//
LOCUS       DADLSA010000331         1443 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.337, whole genome shotgun sequence.
ACCESSION   DADLSA010000331 DADLSA010000000
VERSION     DADLSA010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.337"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            84..425
                     /locus_tag="JN691_004347"
     CDS             84..425
                     /locus_tag="JN691_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY7245553.1"
                     /translation="MLTTSVLNTLIHWVVFGVCIYAAHTSQALANFTGFVVAVSFSFF
                     ANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAISLVCGFV
                     YSKFIVFRDAK"
     gene            422..1351
                     /locus_tag="JN691_004348"
     CDS             422..1351
                     /locus_tag="JN691_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY7245554.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKRLPERNLFMKGILSWVGGQTDVVKYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
BASE COUNT          402 a          281 c          323 g          437 t
ORIGIN      
        1 agaccgcacg aatataacgg tgggttaccc ccaaacgaat cggaaaatcg atactggaaa
       61 aactctaact cggtagccag tttttgttga ccacttcagt gctgaacaca cttatacact
      121 gggtggtttt tggtgtatgt atctatgccg cacataccag tcaggctctg gcaaacttca
      181 caggtttcgt agtggctgtg agctttagct tcttcgcgaa tgcaagattc acattcaaag
      241 catcgactac agcgatgcgc tacatgttat acgtgggatt tatgggaata ttgagtgtga
      301 ttgttggttg ggcagctgat aaatgctcac ttcctccaat agtcactctt atcaccttct
      361 ccgccatcag tctggtgtgc ggtttcgtct attcaaagtt cattgtcttt agggatgcga
      421 aatgaaaata tctcttgtcg ttcctgtctt caatgaagaa gaagcgatac ctgttttcta
      481 taaaacggta cgtgaattcc aagagttgaa gccatatgaa gtagaaattg tattcataaa
      541 tgacggaagt aaagatgcca cagagtcaat tattaacgcg ctggctgttt cagacccgct
      601 agttgttccg ctgtcattta cacgcaactt tggtaaagaa ccagccttat ttgcagggtt
      661 agaccatgca agcggcgatg ctgtaattcc tattgatgtc gacctgcaag acccaattga
      721 ggttatccct catcttattg aaaagtggca ggcaggtgct gacatggtgc ttgctaaacg
      781 ttcagaccgc tcaactgatg gacgactgaa acgtaagaca gctgagtggt tctataaatt
      841 acacaacaaa ataagcaccc caaagatcga ggaaaatgtc ggagattttc gactcatgtc
      901 tcgtgaggtt gtggagaaca ttaaacggtt gcctgagcgc aatcttttca tgaaaggcat
      961 actgagctgg gtgggtggtc agacagatgt cgttaaatat gtacgcgcag agcgtgttgc
     1021 tggcatctca aaatttaatg gctggaaatt atggaatctg gcactagaag gtatcacaag
     1081 tttttcaacc tttcctcttc gcgtatggac ttatataggc ttgtttgttg caagcatttc
     1141 atttttatat ggtgcttgga tgattataga cacccttgta tttggtaacc cagtacgcgg
     1201 gtatccctcc ttgctcgtat caatactttt cttgggtggc gtgcaactga tcgggattgg
     1261 tgttctcgga gaatatatag gcagaatata tattgaagta aaaaatagac ctaaatacat
     1321 cataaaaaaa tctcatcgag gtaacccatg attaaaatta atttatttaa aaattcaaat
     1381 ctccttgcct tcatatcatg ttttgcaata tcaatttatt gttattgggg gtggttgtac
     1441 gat
//
LOCUS       DADLSA010000332         1418 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.338, whole genome shotgun sequence.
ACCESSION   DADLSA010000332 DADLSA010000000
VERSION     DADLSA010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.338"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..183)
                     /gene="fimA"
                     /locus_tag="JN691_004349"
     CDS             complement(<1..183)
                     /gene="fimA"
                     /locus_tag="JN691_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein subunit FimA"
                     /protein_id="HAY7245555.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVR"
     gene            complement(664..1260)
                     /gene="fimE"
                     /locus_tag="JN691_004350"
     CDS             complement(664..1260)
                     /gene="fimE"
                     /locus_tag="JN691_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HAY7245556.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          425 a          305 c          273 g          415 t
ORIGIN      
        1 acgaacctgt cccaactgaa cagtttgatc aacagagcct gcatcaactg cgcaagcggc
       61 gttaacaact tcccctttaa agtgaatggt cccaccattt accgtcgtag tatcggccag
      121 agccgctgcg gaactgaggg acagagccga cagaacaacg attgccagag ttttaatttt
      181 catgctgctt tcctttaaaa aaactatttc taaatcgaca tgggcagtcg ttctgtacac
      241 tttgttttgt caacgagttt gactgccaac actgcacagt tttcccccaa aagatgaaac
      301 atttggggcc attttgactc atagaggaaa gcatcgcgca caaacttttt cagtttattt
      361 gttggcttaa tattctatta ttatctttat ttatagattt ttatattgca tgaggtggtt
      421 tttggagaga agaatgagga agatgcgtcg agccacagaa acgttagctt tacatatagc
      481 ggaggtgatg tgaaattaat ttacaagaga aataatttac atatcaaata gttagatgct
      541 ttttgtcgtt ttttaatatt tttatgcttg agaaaaaata cgtaacttat ttatgatatg
      601 gacagtttgg ccccaattgt cttgtgttta tttgtttttt aactttatta tcaataagtt
      661 aaatcaaacc tcttctcttt ttaatttttc gtttatgaga ttatttcttt cccataatcc
      721 ggcaaaacga gcagcattac tggcggtata acgcacagta tggcgaatat ttcgatgccc
      781 gagataatcc tgaattaaac gggtatctgc accacgctcc gccagttcat aaccgcaagc
      841 atgccttaac atatgaggat gagtctgcgt tacggttcca gcttcaatac cggcatcgcg
      901 aataatgcga taggcctgct ggcgagaaag ccgactcccg cggcgagaaa taaatatagc
      961 gtcggtccgg tcagcgcctt tccagttagc acgttcctgg gtccagcgtt ccacggcttc
     1021 acgctcatca aaacgtaacg ggtgaacggt agaaaatccg ttcttcagtc ggcgaatatt
     1081 tattcgacct tcattaaggt caaggtcctg ataatgcaga tcaagcagtt cactaatacg
     1141 catcccatgc cgatatgcca acagaataag acaataatct ctggctcccg ttgccccgta
     1201 acaaaccgcc tgcatcatgg cctgaacttc tttaccggta agataacgac gtttactcac
     1261 aatagtagta ctcctaactg agatatattt gaatgaatac ctataggaaa cctcaatcgg
     1321 tcaaaattag cccaatagat aaaaagataa ccacacacga agttatgtgg ctatcagtca
     1381 attacattaa acatcataca aaaataaaat atcactga
//
LOCUS       DADLSA010000333         1418 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.339, whole genome shotgun sequence.
ACCESSION   DADLSA010000333 DADLSA010000000
VERSION     DADLSA010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.339"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..780)
                     /locus_tag="JN691_004351"
     CDS             complement(<1..780)
                     /locus_tag="JN691_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7245557.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQ"
BASE COUNT          379 a          229 c          343 g          467 t
ORIGIN      
        1 ttgcagagca tgggatgaca atgggttatc actaatatct attgaacatt cattcctcag
       61 attaagaatg ctttccggga tatgacttat ctgatttatg tcaaaataac cgttccttaa
      121 attttccggc aggtatggaa tatcggttaa catattgtcg attgcccata tggagtgaat
      181 gttgtgaggt agagggggga gttcactaag aagattccct attgcgctaa tctcttgtaa
      241 agaaaatggt aaaggtggta aaactgccag cccattacat gatacatcta atgtttccag
      301 tagttctggt aaaacaggaa gagaacataa ttgattattt gagacatgaa gctccttcaa
      361 agatatgggg agtgtgggta gtgtgattag ttgattgtgg gacgcattca ataatttaag
      421 tccttgaggc aaagcaggca gttcaataag tctgttatag ctgacattaa gctgtgtaag
      481 ggacgcaggc aatggggaga ttaagcttaa attattttta cttatattaa ttgatttaat
      541 tcccgggggg atttcaggta atgttgtcag gcctaactca gacaagtcca ggttcgtctc
      601 ttggttttgt agacatgata ctagtcgctg aaaagcgatg tctcgttgtt cttcttgtat
      661 gcggttattt ttccattcag tccaatgggt tagataattt tcatatgcgc ggctagtgtc
      721 gattgaataa gtggagaacg aatttgaaat taatctgtga ttgttatttg tcgggagcat
      781 aaatatcagg gcgtattctt tatgttgaga ggtgttggat tcttttttat ctgctttgca
      841 atgttattgg ttcccttcag cagtagacag gaacttctgg aggaggcgga tatatagttt
      901 gtgtaagcat attaactcca tatgttatat attgaagaac ttctgcttta cactatccta
      961 aactggatcg gtttaatgta aaaaaacgat actttttggg ggagggggca atttttcctt
     1021 aaggtatgaa aaaaggagct gaagctatat aatagctatt aatgccactg gtatcagtga
     1081 atcatgtata taaaaaactg tgttaaggag gctgtccatt agcggttttt tattcttctg
     1141 gaaccgcggc atctttgcgt atctttttgt cttctcaccg tctggtcggt gttttgctga
     1201 gactgctaat ttcctgtctt tgctagtgct gtactggtac tgcccaatca tgattggcgg
     1261 gggcgggcgg agttgagacc gcagacacgt cgtatgcaag aacgtgctgc ggctggctgg
     1321 tgaacttccg atagtgcgag tattgaatga tttccagccg ttactgattt tacgtgctaa
     1381 ttagtgaaca aaccactcgt cagcagactc ccaggtat
//
LOCUS       DADLSA010000334         1403 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.340, whole genome shotgun sequence.
ACCESSION   DADLSA010000334 DADLSA010000000
VERSION     DADLSA010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.340"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            457..1143
                     /locus_tag="JN691_004352"
     CDS             457..1143
                     /locus_tag="JN691_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HAY7245558.1"
                     /translation="MSELTAKALSDSRSFENAILERDMRMKKAGWIVAALAGLLLVFA
                     LAALILLLPLKTTTVELYTLDRQTGRIERVTTVGEDKLDTSEVLNLSQTASYVKRREG
                     YNYFALQQDYRETQMFNSDEVNKEYLDWFNSPDAPDAVFQQAAYVVTVEVLSNVPSEG
                     TGSSMVEMLRIKRTIRKVKDNTETYDYWTVRMTYRYFPQKKMTASEREVNPFGFIVTS
                     YQRFKEKSDE"
     gene            1136..>1403
                     /locus_tag="JN691_004353"
     CDS             1136..>1403
                     /locus_tag="JN691_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HAY7245559.1"
                     /translation="MNKVLMCALLLTAASAWGAATPRGSAYDSRMQNIPYNSQNTTVI
                     NTRAGYITTLVFDADEEVITATAGFPQGWTITPDRNRVGISPAPV"
BASE COUNT          300 a          457 c          407 g          239 t
ORIGIN      
        1 ggccgaggcg cttgccggtg catccgcaca gggtgccgct caggcacttg ccgcgtcggg
       61 agtaggttca gcccgtcaat ccgcctcctc cgcagccggt gcggtggggg ataaagcctc
      121 acagaacctt aacagcaccg gcagcgcaac ggcctcgtct gcctccacgg cctcccgcgc
      181 caccggcgat gcgcgcagcc gcagccgcgc cgccgttcag cggatgcagg aacgctacca
      241 gagataaccc agtctgactg aactgcggga cgccgcaacg gcgtcctgcg gcttcgtcac
      301 gtctttacca caggaaaaac accatgaaat acggcatcac gctgctgatg ctgtgcctgc
      361 ttgcaggctg cgcacagcga aacacccaac ttccgcccgt gtcgggcaac ccggagccgg
      421 tcaattcccc ggccattatt caggagttaa ccccccatgt ctgaacttac cgccaaagcc
      481 ctgagcgact cgcgctcgtt tgagaacgcc attcttgagc gggatatgcg catgaaaaag
      541 gcgggctgga tcgtggcggc gctcgccggt ctgctgctcg tcttcgccct tgccgccctt
      601 atcctgctgc taccgctcaa aaccaccacc gtggagctgt acacgctcga ccgtcagacc
      661 gggcgcatcg agcgcgtcac caccgtcggc gaggacaagc tggacacctc cgaggtgctc
      721 aatctctcgc agactgcgtc ctacgtgaag cgccgcgagg ggtacaacta tttcgccctt
      781 cagcaggact acagagagac gcagatgttc aacagcgacg aggtcaacaa ggaatacctc
      841 gactggttta acagcccgga cgcgccggac gctgtgtttc agcaggcggc ctatgtagtc
      901 acggtggaag tcctctccaa cgtaccgtca gagggcaccg gctccagcat ggtggagatg
      961 ctgcgcatca agcgcaccat tcgaaaggtc aaggacaaca ccgaaacgta tgattactgg
     1021 acggtgcgca tgacctaccg ctatttcccg cagaagaaaa tgacggcctc cgagcgcgag
     1081 gtgaacccgt tcggcttcat cgtcaccagt taccagcggt ttaaggagaa aagcgatgaa
     1141 taaggtactg atgtgtgccc tcctgctgac cgcagcgtca gcatgggggg cggcgacacc
     1201 gcgcggcagc gcctatgaca gccgcatgca gaacatcccc tacaacagcc agaacacgac
     1261 agtgattaac acgcgggcgg gttacatcac gacgctggtt tttgacgccg acgaggaggt
     1321 gatcaccgcc accgctggtt ttccgcaggg ctggacgatc acacccgacc gtaaccgcgt
     1381 ggggattagc cccgcgccgg tga
//
LOCUS       DADLSA010000335         1399 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.341, whole genome shotgun sequence.
ACCESSION   DADLSA010000335 DADLSA010000000
VERSION     DADLSA010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.341"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            47..721
                     /locus_tag="JN691_004354"
     CDS             47..721
                     /locus_tag="JN691_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7245560.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYRRE
                     FKVRLAKQALQPGAVVARIAREHDINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKHDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIRE
                     MKGGIR"
     gene            718..1065
                     /gene="tnpB"
                     /locus_tag="JN691_004355"
     CDS             718..1065
                     /gene="tnpB"
                     /locus_tag="JN691_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7245561.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            1085..>1399
                     /locus_tag="JN691_004356"
     CDS             1085..>1399
                     /locus_tag="JN691_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7245562.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSE"
BASE COUNT          372 a          361 c          366 g          300 t
ORIGIN      
        1 cctgtctggc aacaatcgcg cccatctata ttgatggaca cgaacgatga attcccagac
       61 aacaaaagat attccctgct tccgttctta tttgcctgat gccctgcgtt taagatttga
      121 agataaactg accatccggg ccatcgctca gcgtctgggt ctcagtcatt ccacaataca
      181 tacgcttttt cagcgatttc ttgcatccgg tatcgcatgg ccattgcccg attcagtttc
      241 attcgctcaa cttgacgcca tcctttatgc caacagaaag aaggaattaa cagagcctca
      301 aatcagagaa ggctcatggc gaaaagaacg gcgaaccagc tatcgccgtg aatttaaggt
      361 ccgtctggct aagcaggcgt tacagcctgg ggctgttgtt gcccggatcg ccagagaaca
      421 cgatatcaat gataacctgc tgtttaaatg gaaaagccag tacgaggacg gcttactgag
      481 cgatgatgac atacaggaat gcatgcctgt cccggtggca ctgactgata cgccggagcc
      541 gaccagacca gttacaaatc ccttctggcg taacaagcat gatgagcgcc ctgagggggc
      601 tcccggaaac gtcccacggt gcgagctgca tcttaaatca ggtgtggtaa aactgtttga
      661 ccctctcact ccggaactgt tacgggcgct aatccgcgaa atgaaagggg gtatccgatg
      721 ataacgctgc cgaccggtac caaaatctgg atcatcgctg gcatcacaga tatgcgttgt
      781 ggcttcaatg gcctggcttc gaaggtgcag aacacgctga aagatgaccc gttctccggg
      841 catatcttcg tcttccgggg ccgcagtggc aaaatggtga aaatactgtg ggccgatcgt
      901 gacgggttat gcctgttcgc caaacgcctg gaacggggcc gcttcgtctg gccggtgacc
      961 cgggaaggga aagtgcacct gacgccagct cagttatcca tgctactgga ggggatcgcg
     1021 tggcaacatc ccaaacggac agaacggcct ggcatccgga tataacccgt gataaaacaa
     1081 gggaatgaac aatgaactcc ccgatgatat tgagctgctt aaagccatgt tgcgtaagca
     1141 acagagtcgg cttcgacagt atgcctgtca ggtcgcgggc tatgagcagg aaattgaacg
     1201 gctgaaagcg caactcgaca ggttgcgtcg tatgttgttc ggccagagtt cagagaaaaa
     1261 gcgtcataag cttgaaaatc agatccgaca ggcagaaaaa cgactgtcgg aactggaaaa
     1321 ccggctgaac acagccagaa atcttctgga agatgcatcg tcagtcacag attcacctga
     1381 caccagtccc ccgtcagaa
//
LOCUS       DADLSA010000336         1398 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.342, whole genome shotgun sequence.
ACCESSION   DADLSA010000336 DADLSA010000000
VERSION     DADLSA010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.342"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            43..630
                     /locus_tag="JN691_004357"
     CDS             43..630
                     /locus_tag="JN691_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245563.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(692..>1398)
                     /locus_tag="JN691_004358"
     CDS             complement(692..>1398)
                     /locus_tag="JN691_004358"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY7245564.1"
                     /translation="EDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMYEYLTTL
                     VKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLTIDNYRE
                     HDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGG
                     DFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPS
                     LMVIADKAAAAELAFG"
BASE COUNT          359 a          324 c          331 g          384 t
ORIGIN      
        1 tactttatct ttgggctact caaaagcaga caggatgtta ctatgactca aaatatcagg
       61 ccgttacccc aattcaaata tcatcccaag ccactggaaa caggcgcatt tgaacaggat
      121 aaaaccgtag agtgcgattg ctgtgaacaa cagacgtcag tttattactc gggtcccttt
      181 tattgcgttg atgaagttga acatctctgt ccgttgtgta ttgcggacgg ttctgctgct
      241 gaaaagtttg caggtagttt tcaggatgat gccagcatag aaggtgttga atttgagtat
      301 gatgaagagg acgaatttgc cggtattaag aacacatatc ctgatgaaat gctgaaagag
      361 ttggttgaac gcacgccagg ttatcatgga tggcaacagg aattctggct tgcgcattgt
      421 ggcgatttct gtgcttttat cggctatgtg ggctggaatg atataaaaga tcgcctcgat
      481 gaatttgcca accttgaaga agattgtgag aatttcggta ttagaaattc tgatctagct
      541 aaatgcctgc aaaagggtgg tgattgtcag ggttatctct tccgctgtct ccactgcggc
      601 aagctgagac tgtggggtga tttttcgtag ttatttaaat aatgagaaca ggccggagcg
      661 taattcatac atccggcctt atttcttcag cttaaccgaa cgctaattct gcggcagcgg
      721 ctttatcagc gattaccatc agcgaaggat gcagctgcaa cacggaagct ggaacatctt
      781 cggtaaccgg accttgcagc acatttttca gtgcctgcgc tttacctgcg ccactgacaa
      841 ttatcagtaa gtttttcgcc gccatgatgc ttttcggccc catcgtcacg tagctgtcag
      901 gcactaacga aaagtcgccg cccaactcac catgcgccac aatatcaacc atctctccct
      961 ggatcgggaa ctccacggtt tgctcatgga aatgggtcgt attcggcaaa ttgccacaaa
     1021 aatgaccatc tgcacctaat cccagcacca ccaaatccag gccgccttca cgcgccagtt
     1081 tctgatcatg ctcgcggtag ttatcaatag tgagcttctg gatattctct tctttgatcc
     1141 ccgcaggggt gaaaaacaga ttacgcagat tggtaatcgt tacgccttct ccctctttgc
     1201 cgcgaaaggg aatttcatcg aaattataga aatagcagtt atcgtaccaa ggcttacctt
     1261 taaccagggt ggtgaggtat tcatacatgc ctttgggcgt gctaccggcg gtaattgcca
     1321 ggttaacacg acgcgtcttc gacatataac caagcagatg gtgtgccgcg acacggctca
     1381 tttcctggta atcttcgg
//
LOCUS       DADLSA010000337         1382 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.343, whole genome shotgun sequence.
ACCESSION   DADLSA010000337 DADLSA010000000
VERSION     DADLSA010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.343"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..212
                     /locus_tag="JN691_004359"
     CDS             <1..212
                     /locus_tag="JN691_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HAY7245565.1"
                     /translation="GHTVLNPAVLPDGLTQPQYMDICMAMIRCVDAIYMLKGWQRSAG
                     AKAELALAEKLGHAVIFQETASVQD"
     gene            199..654
                     /locus_tag="JN691_004360"
     CDS             199..654
                     /locus_tag="JN691_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY7245566.1"
                     /translation="MCKTNYQALRERYSPIQVPECPVCGDEMSIQRIFSRTHIVYACT
                     GEGDDGYFKTGRTFADEHYLKSRVTVVDVSDPDVLALLKELEVKDKRIAELTDVLTQM
                     INAHKTTIRFGHERITECGGDCDSPEKMISENPDIRMAEAVLRAGIKTE"
     gene            752..1018
                     /locus_tag="JN691_004361"
     CDS             752..1018
                     /locus_tag="JN691_004361"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245567.1"
                     /translation="MTELTKEQLIEEAKLKIAIAKCHPNSGVARVEGELFKIALASLE
                     AEPVAWKAIFTQIDHEYNTFTALYSDKAEAERWVRLHKVGDFRA"
     gene            1141..1272
                     /locus_tag="JN691_004362"
                     /pseudo
     CDS             1141..1272
                     /locus_tag="JN691_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459871.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
BASE COUNT          401 a          275 c          376 g          330 t
ORIGIN      
        1 aagggcatac cgtcttaaac ccggcagtac ttccggacgg gctgacacag ccgcagtaca
       61 tggatatctg catggcgatg attcgttgtg tggatgcgat ttacatgctg aaaggctggc
      121 agcggtcagc aggcgctaag gcagaactgg cgctggcgga gaaactgggg catgcggtga
      181 tttttcagga gacagccagt gtgcaagact aattaccagg cattacgtga acgttattca
      241 ccaattcagg tgccggaatg tcctgtttgt ggcgatgaaa tgtcgataca gcgcatattt
      301 tccagaacgc atattgtgta tgcctgcaca ggtgaggggg atgatggata ttttaaaact
      361 ggtcgaactt ttgcggatga gcattacctg aaatcgcgcg taaccgtcgt tgatgttagc
      421 gatcctgacg tactggcttt actgaaagag ctggaagtta aagacaaacg cattgcagaa
      481 ttgacagatg tgcttacaca aatgattaat gcgcacaaaa ccacaattcg ttttggtcat
      541 gaacgcataa ctgaatgtgg tggtgattgc gactcgccgg aaaagatgat ttcagaaaat
      601 ccggatatca gaatggcaga ggctgttttg agagcaggaa taaaaactga ataattaaat
      661 ttagcactgt aaataaaatt taatccttaa ccggagggat ttctgcaccc tcagaacatc
      721 aggaggccgc ccgaaatggc ggtaatgaaa aatgactgaa ttaacaaaag agcaattaat
      781 cgaagaagct aaattaaaaa tagcgattgc gaaatgccat cccaattcag gcgtggcacg
      841 agtagagggt gagttattca aaattgcact ggcatcgctg gaagctgagc cggtggcgtg
      901 gaaggcaatc ttcacgcaaa ttgaccatga atataatacg ttcaccgctc tgtattctga
      961 caaagcagaa gcagaacggt gggtgcgact gcataaagta ggtgattttc gggcataaca
     1021 ccactttatg cagcatcgcc agcgctggta gtgcctgata agttgccgcg tgaatacata
     1081 agaggttggc ctcttgcgta tagtgattat gctgaaggat ggaacgattg tcgtgcagcc
     1141 atgcttcagg gatggcagcc atgccatcaa attaccgcct caatggcaaa agccattgcg
     1201 cttaaacttg gtgctgaact gaataacgaa gaagcagaga ttttcgcgga tgggtataac
     1261 gccgccatgc ttcatgccgg aaactgaacc gtagcaggag gtgaatcgat gagctggcct
     1321 gaagcattca caacggtagg aattgcaatg gcggtggcgc tggttgtgta ttcgatttgc
     1381 cg
//
LOCUS       DADLSA010000338         1377 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.344, whole genome shotgun sequence.
ACCESSION   DADLSA010000338 DADLSA010000000
VERSION     DADLSA010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.344"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            8..808
                     /locus_tag="JN691_004363"
     CDS             8..808
                     /locus_tag="JN691_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAY7245568.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIAHDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTVRTTGRAEATIL
                     VDIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            1005..>1377
                     /locus_tag="JN691_004364"
     CDS             1005..>1377
                     /locus_tag="JN691_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY7245569.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEG"
BASE COUNT          341 a          342 c          346 g          348 t
ORIGIN      
        1 aggaaccatg aacattaccc cgtttccgac gctttcgacg gcaactatag atgccataaa
       61 tgttatcgga cagtggctgg cgcaggatga tttctccggt gaggtgccgt atcaggctga
      121 ttgcgtcata cttgcaggaa atgcggttat gccgactatc gatgcggcat gtaagattgc
      181 tcacgatcag caaattcctt tactgattag tggtggtatc ggtcactcga ccactttttt
      241 gtatagcgcc atcgcacagc atccgcacta caacactgtc cgcaccactg gcagagcaga
      301 agcgaccatc ctggtggata tcgctcatca gttctggcat attccgcatg aaaaaatctg
      361 gattgaagac cagtcaacaa actgcggtga aaacgcacgc tttagcatcg cgctattgaa
      421 tcaggccgta gaacgagttc atacggctat cgttgtacag gaccccacca tgcagcggcg
      481 cacgatggca acgttccgcc gtatgaccgg ggacaatccc gatgcaccac gctggttaag
      541 ttatcccgga ttcgttcctc agttaggaaa taacgcagac agtgtaatct ttattaatca
      601 gttacaagga ttatggccgg ttgagcgtta tctctcacta ctcactggcg agctgccgcg
      661 tttacacgat gatagcgatg gctacggtcc ccgcgggcga gattttatcg ttcacgttga
      721 ttttccggca gaagtcatcc atgcatggca aacgctgaaa catgatgcgg tgctcatcga
      781 ggcgatggaa agtcgctcgt tacgttaaaa attgcccgtt tgtgaaccac ttgtttgcaa
      841 acgggcatga ctcctgactt ttatttctgc cttttattcc ttttacactt gtttttatga
      901 agccctccac agaattgtcc tttcccgatg ccgtctctct gatgattgat gttaattaac
      961 aatatattca ccgaaaacaa acatataaat cacaggagtc gcccatgtca gtacccgttc
     1021 aacatcctat gtatatcgat ggacagtttg ttacctggcg tggagacgca tggattgatg
     1081 tggtaaaccc tgctacagag gctgtcattt cccgcatacc cgatggtcag gccgaggatg
     1141 cccgtaaggc aatcgatgca gcagaacgtg cacaaccaga atgggaagcg ttgcctgcta
     1201 ttgaacgcgc cagttggttg cgcaaaatct ccgccgggat ccgcgaacgc gccagtgaaa
     1261 tcagtgcgct gattgttgaa gaagggggca agatccagca gctggctgaa gtcgaagtgg
     1321 cttttactgc cgactatatc gattacatgg cggagtgggc acggcgttac gagggcg
//
LOCUS       DADLSA010000339         1358 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.345, whole genome shotgun sequence.
ACCESSION   DADLSA010000339 DADLSA010000000
VERSION     DADLSA010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.345"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            210..998
                     /gene="virF"
                     /locus_tag="JN691_004365"
     CDS             210..998
                     /gene="virF"
                     /locus_tag="JN691_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HAY7245570.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(940..1261)
                     /locus_tag="JN691_004366"
                     /pseudo
     CDS             complement(940..1261)
                     /locus_tag="JN691_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741395.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          443 a          230 c          247 g          438 t
ORIGIN      
        1 gtccctttca gtgcaaatac ttagcttgtt gcacagagaa atagaagctg cataagctct
       61 ttcttcaaaa aatgtaaata aagttaaata taggaaaaat tacttaatct atcttaataa
      121 cggaaagatt ttgtatacaa tcactgttac agaaatttct tagttactct gtaaacacta
      181 aatatagttt ggtatattct gttgaattta tgatggatat gggacataaa aacaaaatag
      241 atataaaggt tcgcttgcat aactatatta ttttatatgc aaaaaggtgt tcaatgacgg
      301 ttagctcagg caatgaaact ttgactatcg atgaagggca aattgctttt atagagcgaa
      361 atatacaaat aaacgtctcc ataaaaaaat ctgatagcat taatccattt gagattataa
      421 gccttgacag aaatttatta ttaagcatta ttagaataat ggaaccaatt tattcatttc
      481 aacactccta ttctgaggag aaaagggggt taaacaaaaa aatattcctc ctctctgagg
      541 aggaggtttc tatcgatttg ttcaaatcta taaaagagat gcctttcggc aaaagaaaga
      601 tctatagttt agcttgcctt ttatcagctg tttctgatga ggaagcttta tatacttcga
      661 tatcgatagc ttcttctctt agtttttctg atcagataag gaagattgtt gaaaaaaaca
      721 tcgagaagag atggcgtctt tctgatattt caaataactt gaatttatca gaaatagctg
      781 ttagaaaacg attggagagt gaaaaattaa catttcaaca aatccttctt gatattcgca
      841 tgcatcatgc agcaaagctt ttattgaata gtcaaagcta tattaatgat gtatcaagac
      901 ttatcggaat atcaagccca tcttatttta taaggaaatt taatgaatat tatggtataa
      961 ctccaaagaa attttactta tatcataaaa aattttaaat gcttcatagc ccatcgctat
     1021 tgccagatgg gttttcccca cacctgatgg ccccagtaat acgatatttt cattacgttc
     1081 tatgaagctg agtgagcgta acgactggag ttgcttctgc ggtgctccgg tggcgaatgt
     1141 gaagtcatac tcttcgaacg ttttcaccgc cgggaaggct gccattcggg tatacatcgc
     1201 ctgtttacgt tgatgacgtg ccagtttttc ttcatgaagc atgatgctcc aggaagtcca
     1261 tataactcca ttcctggtct actgcctgtt gtgacagcgc aggcgctgcg cttatgtact
     1321 gcacccattt tgttggacga tgaaatggaa tagcccct
//
LOCUS       DADLSA010000340         1340 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.346, whole genome shotgun sequence.
ACCESSION   DADLSA010000340 DADLSA010000000
VERSION     DADLSA010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.346"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            1..272
                     /locus_tag="JN691_004367"
                     /pseudo
     CDS             1..272
                     /locus_tag="JN691_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611999.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            289..807
                     /locus_tag="JN691_004368"
                     /pseudo
     CDS             289..807
                     /locus_tag="JN691_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          405 a          307 c          294 g          334 t
ORIGIN      
        1 gtgatgagcg gctaaaactg gagattaagg tggcacatat ccgcactcgc gaaacatatg
       61 gaacccggcg gctccagacg gagctggcag ataacggaat tatcgtcggt cgtgacagac
      121 tggcacgtct tcgtaaggag ctgaggctgc actgtaagca gaaacgcaag ttcagggcga
      181 ccacaaactc tgaccacaat ctgccggtta cgccgaatct gctgaatcag aactttacgc
      241 ccacagcacc taatcaggtc tgggtcgcag attcagttgt ccaggccttc cgaaatcaac
      301 cgactgaagg cgctggtcgc gaaactgcag cgtatgcagt tcggtaaaag ctcagaaaaa
      361 cttcgcgcaa aaaccgaacg gcggatacag gaagcacagg agcgaatcag cgcacttcag
      421 gaggaaatgg cggaaacact gggtgagcaa tatgacccgg tactgccatc ttccctgcgc
      481 cagtcttcag cccgtaaacc gttaccagcc tcacttcccc gtgccccccg ggtcatccgg
      541 ccggaagagg aatgctgtcc ggcctgtggt ggtgaactca gtccactggg atgtgatgtg
      601 tcagagcagc tggagcttat cagcagcgcc tttaaggtta tcgaaaaaca acgccctaaa
      661 ctggcctgtc gccggtgcga ccatatcgtg caggcaccgg tgccttcaaa acccattgca
      721 cgcagttatg ccggagcggg gcttctggcc catgttgtta ccggaaagta tgcagaccat
      781 ctgccattat accgccaaag tgatctacta ttccacacag ctatttagct ctctttttgt
      841 tgagctccca tgattctttc atttattgtt actaattatt ttatgttatc atgatgttag
      901 aacacgctct gctctgtgtc tctattttat ctatcatatt ctgaggatta actacataac
      961 aaggcgcaag atatatatcc aaaaacaaat aataccgttg caataattcc tttcagtaaa
     1021 ggaggtgtga ttaatttcag gggatttcag agtgaaaatt gtacaacata ttaaaatatg
     1081 ttcatttcgt tataatacac acaagaaaat attaagttag ataatatatt ctaaataacc
     1141 atagataaaa acgcacataa tggggaatat tttatgaaaa tacctgaagc agtaaatcat
     1201 attaatgttc agaataatat tgatcttgtt gatggaaaaa cgaatccgaa taaagcaaca
     1261 aaagcattac aaaaaatata ttacgcgtaa caaactcatc ttcttctggc ataagtgaaa
     1321 aacatttaga tcactgtgct
//
LOCUS       DADLSA010000341         1281 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.347, whole genome shotgun sequence.
ACCESSION   DADLSA010000341 DADLSA010000000
VERSION     DADLSA010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.347"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..1019
                     /locus_tag="JN691_004369"
     CDS             <1..1019
                     /locus_tag="JN691_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HAY7245571.1"
                     /translation="FSCTINYVQSLRELYTDVIEEFYWVALPLTTQNSLSQYQPEWQC
                     WEPDVEWVRQPPQDAITAPDFFCFYQPGMTFEQFVREFAEWFSQKRPAAMMIGIRADE
                     SYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTWYANHQNLCNP
                     LYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVSGVKSGGIYAG
                     HDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHWYQKKGIEVPQ
                     TQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERIKGKRQEWGIL
                     CNND"
     gene            992..>1281
                     /locus_tag="JN691_004370"
     CDS             992..>1281
                     /locus_tag="JN691_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAY7245572.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDK"
BASE COUNT          371 a          313 c          291 g          306 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct ggtatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaagccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag cccctttcgt gaagagccag tggattgcgt
     1141 tctgtgggtt aaaaacagcc aacttatgcc taatgattac aacccaaaca acgtggcacc
     1201 acccgaaaaa aaactgctgc aaaaatccat tgaaatagat ggttttaccc aacccattgt
     1261 ggtcacacat acggataaaa a
//
LOCUS       DADLSA010000342         1261 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.348, whole genome shotgun sequence.
ACCESSION   DADLSA010000342 DADLSA010000000
VERSION     DADLSA010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.348"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..84
                     /locus_tag="JN691_004371"
     CDS             <1..84
                     /locus_tag="JN691_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7245573.1"
                     /translation="RDELIDTTQARPIQYCINGTWYNAGSI"
     gene            87..620
                     /locus_tag="JN691_004372"
     CDS             87..620
                     /locus_tag="JN691_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7245574.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGIVI
                     KRTYTGEEQRQQMENEKQSLLQLVRDKTQLWDSQLRLGIISAGNKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(649..1176)
                     /locus_tag="JN691_004373"
     CDS             complement(649..1176)
                     /locus_tag="JN691_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7245575.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKNFDVIWLWTEDGKNWYGEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERSVRLNMATDEERARLESWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(1178..>1261)
                     /locus_tag="JN691_004374"
     CDS             complement(1178..>1261)
                     /locus_tag="JN691_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7245576.1"
                     /translation="SADNIAGVYYRPIQKYINGTWYNVASV"
BASE COUNT          360 a          282 c          276 g          343 t
ORIGIN      
        1 agagatgaac taattgatac aactcaggca aggccaattc agtattgcat taatgggacg
       61 tggtataacg cggggagtat ttaacgatga tgcacttaaa aaacatttct gctggtaatc
      121 ctgaaacaaa agagcaatac cagctaacga aacaatttaa catcaaatgg ctttatacag
      181 aggatgggaa aaactggtat gaggaacaaa agaactttca gcctgatacg ttgaaaatgg
      241 tctatgacca caacggcgtt attatttgta ttgaaaagga tgtttcagca attaatccgg
      301 aaggcgcaag cgtcgttgag gttcctgata ttacagcaaa tcgccgggct gatatttcag
      361 gtaaatggat gttcaaagat ggcatagtga taaagcgaac ttataccggg gaagagcaga
      421 ggcaacaaat ggaaaatgaa aagcaaagcc tgttgcaact tgtcagggat aaaacccagc
      481 tatgggactc acagctacgg ctgggcatca tttccgccgg gaataagcag aaattaaccg
      541 agtggatgct ctacgcgcag aaggtcgaat ccacagacac ctccagtctg ccagtaacgt
      601 ttcccgaaca accagaatga gagaaggccc gctatcgggc cttaattttt actctggttt
      661 ttgtggccat tctggctttg ccgtatccac acggctgacc agaacactgt agcgttccca
      721 tgactccagt cgtgcgcgtt cctcatctgt tgccatattc agcctgacag agcgctccag
      781 cggctggatg actgattcag cttcggaaag caatgcggct ttttgtgatt cggcctgttg
      841 ctgctgttcg tctgccgtat aaatccgctt aaccacagct ccgtccttaa acatccactt
      901 cccggaatca tcggcacgac gatttgctgt tatatcgggg acctcaacga cgctaaagcc
      961 ttcaggatta agcgtggagg catctttagt gatggcgaca ataatattat tttcatcgta
     1021 aacaatcttt attgtatctg gctgaaagtt cttcacttcc ccataccagt tttttccgtc
     1081 ttctgtccac aaccagatta cgtcaaaatt ctttgttaat tcgtactgtt ccagtgtttt
     1141 agcgttaccc gctttaatgt tctttaagtg catcatatta aacgctcgct acattatacc
     1201 aggtgccatt tatatacttt tgaatcggtc tgtaataaac acccgcaata ttatcggcag
     1261 a
//
LOCUS       DADLSA010000343         1247 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.349, whole genome shotgun sequence.
ACCESSION   DADLSA010000343 DADLSA010000000
VERSION     DADLSA010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.349"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="JN691_004375"
     CDS             complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="JN691_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HAY7245577.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNV"
BASE COUNT          352 a          228 c          271 g          396 t
ORIGIN      
        1 ttaacattcg tacaaatggg caatgtgatt atattttcgg gtaatgaagt tagtaggtta
       61 ttggaaacat ccagtgccaa tagttcttgc ggtaatctgg ataatctgcg tagttgattt
      121 tcacctacct tcaaatattt taagcgctga ggaaattctg gtagttcctg tagtctgttg
      181 ccggaaacaa agagttcttg tagtctatga ggcaattcag gcaagacagt aaggcgatta
      241 ccttcaatac gtaatattgt tagcgttgat ggaagctctg gtaacgtctc cagactatta
      301 aaacgtgccg agagtgattg taaatatggt ggtaatgatg gcagacatga caaatttcga
      361 ttgaatgaaa tattaagctt aatcagttga gatggaagtg tcgggagtat attcaagttg
      421 ttaccattta catgaagttc tgtcaaaagc ggagaatttt caggtaggga gattaactca
      481 ttgttggaaa tattaagttc acgtatatgc aaagggagtg gtggtaaaga acgtagtttc
      541 agtaagctta aattgagaac aggctcccgg gtatccagac atattttcaa taatcgaacc
      601 gcctggatgc gattttctgt tgttgttcct tccctagccc agtcatccca gatgcggtgg
      661 taatctctgg tacactgttc ttctaccgat aaacgagata tacatgataa gccacaaagg
      721 gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt aacccctcac tattcatatc
      781 ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa aaataatcct agagctttat
      841 cggcgttttc attccagctg gaggaatatg ttcctgtctt tccagtaacc gtcgattgtt
      901 atatcagtct acccatgtta atgtgaccag ttctactttc atccagtttg gattgatctt
      961 tatcccctat attttcaaac aatttttatc atttaactca ttactgtctc gatattccca
     1021 tagcaaacga tctggttggt tagcccctca aaaaagtcag ttgcacgccg taaatccctg
     1081 ccggtattga gcgaggattt acgggttgtt gttaaggtcc gtaatcgggt cacagttttt
     1141 ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt gattatgaat ggtgcagtcg
     1201 tgagccgttt tcagacaatc gcagggccag tacctcgtca gtcaact
//
LOCUS       DADLSA010000344         1241 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.350, whole genome shotgun sequence.
ACCESSION   DADLSA010000344 DADLSA010000000
VERSION     DADLSA010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.350"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..689)
                     /gene="ipaH7.8"
                     /locus_tag="JN691_004376"
     CDS             complement(<1..689)
                     /gene="ipaH7.8"
                     /locus_tag="JN691_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HAY7245578.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRI"
     gene            1019..1198
                     /locus_tag="JN691_004377"
                     /pseudo
     CDS             1019..1198
                     /locus_tag="JN691_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          405 a          179 c          247 g          410 t
ORIGIN      
        1 ttaatcctgt aacttgaatc taattgtgaa aaactctccg gaagagtggt tatctgatta
       61 aaatgaaaat aatattcctt ttgtctgata ttgtttctat cagaaaattg aggaaaagta
      121 accagattat tgcgctgagc acagagaatt tccagtttat ccggtaagtc gggtaatgtt
      181 gtcagcctgt tataataaac atgtaaaaat ttcaattttt cgggaagagc cggtaagtta
      241 tatagcctgt tattttcaac aaataatgat gttaaatggt ttggcaaaac aggaaggttt
      301 tccagttgat tagaacgaac acttaaagtt gttaatgact caggaagttc tggtatcaca
      361 gaaagcctgt tattatcagc attaagttct ttgagaaatg caggcagctt tggcaatgat
      421 gttaatttat tattttctac aatcaaagca gaaatatgct ttggtaattc gggaagagac
      481 gtcaaattta ggtctgataa atttaatact gcttcttgat tctgaaagca ctgggagagt
      541 ctgttaaaag caatgcctcg ctcttccccg ggagaagagt tcttttccca ttcagtcagg
      601 attctcagat aatgttcatt actggttgag tttgagttaa tagaggggga gcaagaaact
      661 gatgagtgtg tattatttac agagaacata atgctaggtc aaccttatat ttgtgagaat
      721 aaatatttaa aattgtatat ttatacaaat ttcacttacc atttccgatg ttaagtttta
      781 atagtttcca ttttacacat aacagaagtt atgttgtaat ttatttattg aggttttcta
      841 tggaattctt tttttattgt gactgcagtc aataataaca ctgaattcaa cattccagtg
      901 agcaaggaaa tgtaatccgg gtaagtatat ttgttttaca tcatatttta gccaagctca
      961 tttattccta ttatagaatg ataaattaaa attactgtaa aaaaacgata cacatttaat
     1021 acgagggagg ccggcagaaa aacggcttgc ggtaaggcag gagaaatgtg tgccgcgaat
     1081 gaagtccttg tatgactgga taaagctcca gaggaaaact ttgtcaaacc atgctgaaat
     1141 ggtaaaggcg ttcaactata tcctgaatca ctggaatgca ctgaatgagt tttgccgggt
     1201 agtacttcca atcattagaa tatgtgcttt tcaatgaagg a
//
LOCUS       DADLSA010000345         1238 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.351, whole genome shotgun sequence.
ACCESSION   DADLSA010000345 DADLSA010000000
VERSION     DADLSA010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.351"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..>1238
                     /locus_tag="JN691_004378"
     rRNA            <1..>1238
                     /locus_tag="JN691_004378"
                     /product="23S ribosomal RNA"
BASE COUNT          314 a          280 c          382 g          262 t
ORIGIN      
        1 acggtgctga agcaacaaat gccctgcttc caggaaaagc ctctaagcat caggtaacat
       61 caaatcgtac cccaaaccga cacaggtggt caggtagaga ataccaaggc gcttgagaga
      121 actcgggtga aggaactagg caaaatggtg ccgtaacttc gggagaaggc acgctgatac
      181 gtaggtgaag cgacttgctc gtggagctga aatcagtcga agataccagc tggctgcaac
      241 tgtttattaa aaacacagca ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct
      301 gcccggtgcc ggaaggttaa ttgatggggt tagcgcaagc gaagctcttg atcgaagccc
      361 cggtaaacgg cggccgtaac tataacggtc ctaaggtagc gaaattcctt gtcgggtaag
      421 ttccgacctg cacgaatggc gtaatgatgg ccaggctgtc tccacccgag actcagtgaa
      481 attgaactcg ctgtgaagat gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct
      541 ttactatagc ttgacactga acattgagcc ttgatgtgta ggataggtgg gaggctttga
      601 agtgtggacg ccagtctgca tggagccgac cttgaaatac caccctttaa tgtttgatgt
      661 tctaacgtgg acccgtgatc cgggttgcgg acagtgtctg gtgggtagtt tgactggggc
      721 ggtctcctcc taaagagtaa cggaggagca cgaaggttgg ctaatcctgg tcggacatca
      781 ggaggttagt gcaatggcat aagccagctt gactgcgagc gtgacggcgc gagcaggtgc
      841 gaaagcaggt catagtgatc cggtggttct gaatggaagg gccatcgctc aacggataaa
      901 aggtactccg gggataacag gctgataccg cccaagagtt catatcgacg gcggtgtttg
      961 gcacctcgat gtcggctcat cacatcctgg ggctgaagta ggtcccaagg gtatggctgt
     1021 tcgccattta aagtggtacg cgagctgggt ttagaacgtc gtgagacagt tcggtcccta
     1081 tctgccgtgg gcgctggaga actgaggggg gctgctccta gtacgagagg accggagtgg
     1141 acgcatcact ggtgttcggg ttgtcatgcc aatggcactg cccggtagct aaatgcggaa
     1201 gagataagtg ctgaaagcat ctaagcacga aacttgcc
//
LOCUS       DADLSA010000346         1228 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.352, whole genome shotgun sequence.
ACCESSION   DADLSA010000346 DADLSA010000000
VERSION     DADLSA010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.352"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..131
                     /locus_tag="JN691_004379"
     CDS             <1..131
                     /locus_tag="JN691_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7245579.1"
                     /translation="DREKLKAYRIYAKSLQAMDFSVITDKTSYNAIEWPASPEISS"
     gene            155..>1228
                     /locus_tag="JN691_004380"
     CDS             155..>1228
                     /locus_tag="JN691_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7245580.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMHTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWHNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKTAN
                     HLSEIAAAGEKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYR
                     AKSLTFYGINGPIYHTRYCYWPISRLTGWVKINITTEDIIYRIVASSVRNRWGDPDIG
                     GLIIAAYQGEADGDKVIRLVRGQS"
BASE COUNT          325 a          300 c          341 g          262 t
ORIGIN      
        1 aagaccggga aaaactgaaa gcgtaccgca tttacgcgaa atcgctgcag gcgatggatt
       61 tcagtgtcat cactgataaa acctcataca acgccattga atggcccgct tctccggaaa
      121 tttcttcctg atttaattta tcgcgagaaa aactatgtct gtagtgatat caggtgcgct
      181 gactgatggc gcaggtatcc ccatgtccgg ataccatatt attctgaaat cccgggtaaa
      241 caccccggaa gtggtgatgc acactgttgc tgatgtgatg acaggaaacg atggtgaata
      301 ctgtttccat gcgcggactg gaaaatatgg tgtgtatctg aaacaggact ggcacaacga
      361 gtacaacgtt ggcgacattg ctgtatatga ggactcaaag cccggcacgc tgaatgactt
      421 tctgattgct cctgatgagg gcgacctgaa accggatgtc gtcaaacgct ttgaggaaat
      481 ggtggcgcag gcgcagcaga gcgccggggc cgcagccgga aacgcacagc agacggcgca
      541 ggatgtggcg gcagccgcag gttatgcccg cgcagcagag caggccaaaa atgacattga
      601 tgctgcgctg accggcactc tgaaaacggc taaccatctg tctgaaatcg cagcagcagg
      661 cgaaaaggca caacagaagt cccgggataa tctggggctg aaaagtgcgg ccacgatgga
      721 agcacagagc gacatttacg accggacaaa aggccgtctg gcgatacccg gcgcattcgg
      781 ctttgggtgt gcttttctgc ctgaagatgt tatccgtttt gacactaaga gtgatttcct
      841 ggcctgggta aggaatgcgc tgccaggtga atattccgtt gctggcccct acggcatcat
      901 catacccgac acacggtttg aaggggtgct cagcatccgg tggactgatg cacgccctga
      961 gacaacagaa ccgaggtaca gagccaaatc ccttactttt tacggcatta acggccccat
     1021 ttatcacacc cgctactgct actggcccat atccagactg actggctggg tgaaaataaa
     1081 tataaccaca gaagatatta tttacagaat cgtggcgagc tctgtccgca acagatgggg
     1141 agaccctgac attggcgggc tgattattgc tgcgtaccag ggagaagctg acggtgataa
     1201 agtcatcaga cttgtcaggg ggcagtca
//
LOCUS       DADLSA010000347         1213 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.353, whole genome shotgun sequence.
ACCESSION   DADLSA010000347 DADLSA010000000
VERSION     DADLSA010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.353"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(11..202)
                     /locus_tag="JN691_004381"
     CDS             complement(11..202)
                     /locus_tag="JN691_004381"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245581.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASLAEVYWQ"
     gene            339..1213
                     /locus_tag="JN691_004382"
                     /pseudo
     CDS             339..1213
                     /locus_tag="JN691_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063511.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          319 a          305 c          351 g          238 t
ORIGIN      
        1 gtagtgtccg ctattgccag tacacctcag ccagggatgc cggcacatct gccagtgagg
       61 cggcaaccaa tgcgactgat gctgcaggct cagcacgcgc agccagcacg tcagccggtt
      121 cggcagcgtc atcggcatca tctgcgtctg cttcaaaaga tgaggcgact aaacaggcgt
      181 cagcagcgaa gggcagtgcc acgacggcat cctctaaggc gacagagtct gcaggcagtg
      241 cgacggcggc agcgcagagc aaaagcgcgg cagaatccgc ggcaatgcgc gctgagacag
      301 cagcaaaacg ggcagaggat attgctgatg tcgtatctct ggaagatgcg agcacgacga
      361 aaaaggggat agtgcagctc agcagtgcga ccaacagtgc atccgagtca ctggccgcga
      421 cagcgaaagc ggttaaggtg gtaatggatg aaacaaacaa gaaagcgccc ctgaacagtc
      481 cggctctgac cggaacgcca accacaccca ctgcgccaaa ggggactaat aatactcaga
      541 tcgccagtac ggcttatgtt atggccgcga ttgctgccat cgtggactcg tcgcctgatg
      601 cactgaacac gctgaatgaa ctggcggcgg cgctgggcaa cgacccgaat tttgcgacca
      661 ccatgactaa cgcgcttgcg ggtaagcaac cgaaagatgc cactctgacg gcgctggcgg
      721 ggcttgctac tgcggcagac aggtttccgt attttacggg gaatgatgtc gccagcctgg
      781 caaccctgac aaaagtcggg cgggatattc ttgcgaaatc gaccgttgcc gccgttatcg
      841 aatacctcgg tttacaggaa acggtaaaca aggctgataa cgctgtccaa aagacaggcg
      901 ataccttgtc cggtggactt acttttgaaa acgactcaat ccttgcctgg attcggaata
      961 ctgactgggc gaagattgga tttaaaaatg attcggatgc agacaccgat tcctacatgt
     1021 ggtttgaaac ggcggataac ggtaatgaat atttcaaatg gagacacgcc tcgctggcgg
     1081 ccgggttaaa gacctgatga atctcaaatg ggatacacta aatattctgg ttaatgccgt
     1141 cattaatggt tgccttggca ttggtacgac gaatgcgtta ggtggaagtt caatcgtttt
     1201 aggagataac gat
//
LOCUS       DADLSA010000348         1204 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.354, whole genome shotgun sequence.
ACCESSION   DADLSA010000348 DADLSA010000000
VERSION     DADLSA010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.354"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(11..>1204)
                     /locus_tag="JN691_004383"
     CDS             complement(11..>1204)
                     /locus_tag="JN691_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HAY7245582.1"
                     /translation="VFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVASYLKDHL
                     CPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAANMPLYQLL
                     GGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYGLAKGKGL
                     AYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLTPIEAARF
                     GKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDY
                     IRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWVPNFGVQE
                     YMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLPVARLEDG
                     TLLNW"
BASE COUNT          315 a          332 c          276 g          281 t
ORIGIN      
        1 cgaaccgtta ttaccagttc aacaatgtgc catcttccag tcgtgcgacg gggagatacg
       61 ccgggtcata cggatatttc gccgccagtt tctcatcgaa agaaataccc agccccggtt
      121 catcgcctgg gtgcatataa ccttcctcga agcgccagct gtgcgggaaa acttcaagca
      181 tttgttcgga atagcccatg tactcctgaa cgccaaaatt tggtacccat aggtcaaaat
      241 gcagcgccgc cgcatgacaa acaggcgata aatctgaagg gccatgcgaa ccggtacgta
      301 cctgataaag cgatgcaaaa tcggcaatcc gccgcatccc ggtaatgccg cctgcatggg
      361 tgatcgtcgt gcgaatatag tcaatcagct gctcttcgat taactgcttg cagtcccaga
      421 tactgttaaa cacttcaccg accgcaatcg gcgtcacggt atgctggcga atcagacgaa
      481 aacattcctg gttctcggca ggcgtcggat cttccatcca gaacaggcgg taatcctcaa
      541 tgctcttccc aaagcgcgcc gcttcgatgg gggtcaggcg atggtgcatg tcatgcaaca
      601 gatgttcgtt aaaaccgaac ttattacgca ccgcttcgaa taacttcggc gtaaaatcga
      661 gatacttttc tgaagaccac aactgctctt ctggccacag gcctttggtt gccggttcat
      721 acgccagtcc tttgcccttc gccaggccat aagtggtttg catacccggt acaccgcact
      781 gcacacgaat cgccttaaag cccatctgct ggtgctttgc atagtcttca aggacttcat
      841 caatcgtacg cccggtggtg tggcaataaa ccatcacgcc ttcacgagaa gcaccaccaa
      901 gcagttgata caatggcata tttgccgctt tagctttgat atcccacaac gccatatcaa
      961 tggcagaaat ggctgacatc gtgacggggc cacgtcgcca gtaagcacct ttataaaaga
     1021 attgccagat atcttcgata cggctggcat cgcggccaat tagttgtgga caaagatgat
     1081 ctttcagata agaagcgacc gacaattcgc gaccatttaa cgtggcatct cctaatccac
     1141 aaagtccgct ttcagtggtt attttaagcg taacgaaatt acgccctggg caagtaacaa
     1201 aaac
//
LOCUS       DADLSA010000349         1194 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.355, whole genome shotgun sequence.
ACCESSION   DADLSA010000349 DADLSA010000000
VERSION     DADLSA010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.355"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(40..549)
                     /locus_tag="JN691_004384"
     CDS             complement(40..549)
                     /locus_tag="JN691_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAY7245583.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            678..1007
                     /locus_tag="JN691_004385"
     CDS             678..1007
                     /locus_tag="JN691_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HAY7245584.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGCLTIIADSDEVQELREELYQVKQVVKGMRDGM
                     FSVLNES"
BASE COUNT          350 a          249 c          310 g          285 t
ORIGIN      
        1 gcagacgtaa atcctggcga aaccaggcca gatgggtagt cataaaactc ctgaatggcg
       61 caagttaatc acacgcggcc aaagttcgaa ataacgctgc cctgaaagcc aggcgtcagg
      121 ataaagcgcc atatagtgtt tgatacgcat cagcggcgca agaagtggtt gctcaccacg
      181 gcgataactg tcgataagcg cagccagctg ctggcgttct gtggaatcaa tatgcgggcg
      241 aaaataaccc tgaacgtgca tcaaaacgtt ggtgtgatcg cgcgggttgg cgggatgaga
      301 aagcaaaaca attacgcgtt ggcggtactg gttgtaaaaa tcatccagat tttgccactg
      361 atgaatatca gccacaaacg ggccaagttt gcggtactcg ggctgagaat gggccagaaa
      421 gacgagttta tagcggctgt gataatcgag aagcgccccg cgcgttaagc catgttcttt
      481 cagtcgatgt aactcatgaa gggcgaagat acggattagg gtgctgtcat caaatcgttg
      541 tagattcatt actgctcctg aacggtatac aggagtaagt gtagacgttg gtaaatgatg
      601 tttgtgaagt taatccgcta gcagggcaag attacgaaag cgcgctcccc gcaagcaact
      661 aatcaaggta gttaatcatg gctgaacacg atattacgcc agaagtcacc atttccaaaa
      721 cacagcgccg ctataaagtc ggctatgtca gagcccgcca ccaggaccgc aaaaccggta
      781 tagcccggta ttattcacaa catcccagcc tgcaccttaa gggcgactgg ctgaaagagg
      841 ccggatttga taccggacgg ggtgtcacgg tgaagatttc ggaggggtgc ctgaccatca
      901 tcgctgacag tgacgaggtg caggaactgc gggaggaact ttatcaggtt aaacaggtag
      961 ttaaggggat gcgggatggg atgtttagtg tgctgaacga aagttaaata ttaaaaaagc
     1021 tgggaagatt taaaaccttc ctagcttttt attctgtctt attataatgg atactcatca
     1081 tcttccgatg aatttataaa ttgctccaag tttacatgaa gagtacttaa cctagttttt
     1141 aactcattaa gtgaagcgat aatttcatat tttccaacat aatcatatga tgga
//
LOCUS       DADLSA010000350         1144 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.356, whole genome shotgun sequence.
ACCESSION   DADLSA010000350 DADLSA010000000
VERSION     DADLSA010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.356"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            293..1135
                     /locus_tag="JN691_004386"
     CDS             293..1135
                     /locus_tag="JN691_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245585.1"
                     /translation="MKKELFGRCPFDLDEDVKKIAAINKYIHAKFEEHKNKIDNLKGV
                     EQKLMVLQYFNVLNELSNQAISSLNIGAFSASEVLARVIIEQAANQFYIAIDDGENAQ
                     ALLKASKKLVHSNGKRWLECLKSKGMTNPAANERIRMGKELTDLFNRLWPNTPEYPGT
                     RKLFELIGWETHYHAHYVPLCDSIHTFSDDMVNIVSLYNAIQSDETTAIEFILAVKQE
                     NKRLAIYNFVIATALRCEALVNVFNSLGYQDIITEMTPTIDTVNQTIIRYDDFDHSRI
                     DDCQ"
BASE COUNT          389 a          197 c          221 g          337 t
ORIGIN      
        1 ctgtagatcg ctgtgtgttg catctatcgg ttgaactcac agcccaaacc agactgtcag
       61 ctttgattag attctaccta ctcaacctgt cagataaggt ctgagctaat acatctaata
      121 tatggctttg tgcaggggac ggctatcgcg aacgttaggc taggtatctg aatacgcaca
      181 gttcacttgg ttgtacttct tgcactgatc tgctatcccg cacagctatt tataggtaat
      241 cttcagtgta taatctgccg acttatggaa gcttgtacaa caggagaaat atatgaaaaa
      301 agaattgttt ggacgttgcc cgtttgatct cgacgaagat gtaaaaaaaa tagccgcgat
      361 aaataaatat atccatgcaa aatttgagga gcataaaaat aaaatagaca acctaaaggg
      421 tgtggaacaa aaattgatgg tcttacagta ctttaatgta ctgaatgaac ttagtaatca
      481 ggctatctct tctttgaata taggagcctt ttcagcatcg gaagtattag ctagggttat
      541 aatagaacag gctgccaatc aattttatat agcaatagat gatggcgaaa atgcacaagc
      601 attactcaag gctagtaaga aattagttca tagtaatgga aaacggtggc tggagtgttt
      661 aaaatccaaa ggaatgacta atccagcagc aaatgaaagg attcgcatgg gaaaagaact
      721 aactgatcta tttaatagat tgtggccaaa tacacctgaa tatccaggaa ccagaaaact
      781 ttttgaatta attggctggg aaacacacta tcatgcccat tacgttcccc tttgtgattc
      841 aatacataca ttctctgatg atatggtaaa tatagtatct ctatataatg caatccaaag
      901 tgatgaaact actgctatcg aattcattct tgcagttaaa caagaaaata aacggctggc
      961 aatttataat tttgtaattg ctacggccct tcggtgtgag gccttagtaa atgtatttaa
     1021 ctctttaggt tatcaggata ttataacaga aatgacacca acaatagata cagtcaacca
     1081 gactataatt cgatatgatg attttgatca ttccagaatt gatgattgtc aataattaat
     1141 tgac
//
LOCUS       DADLSA010000351         1139 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.357, whole genome shotgun sequence.
ACCESSION   DADLSA010000351 DADLSA010000000
VERSION     DADLSA010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.357"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(1..265)
                     /locus_tag="JN691_004387"
                     /pseudo
     CDS             complement(1..265)
                     /locus_tag="JN691_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(380..1018)
                     /locus_tag="JN691_004388"
     CDS             complement(380..1018)
                     /locus_tag="JN691_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245586.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          332 a          280 c          174 g          353 t
ORIGIN      
        1 ccagcaagcg ctgtaactct tttatttgtt ttatagcggc agccagttct gaggcaggaa
       61 cgacatcctc tccggcagca acagcagtga gacttccttc cagatattat tttttccact
      121 taagcaacag gctgggctga ataccatgca aacgcgccac atgggacacc gtcatgccag
      181 gctccattgt ctgctgaata atggcaattt tttcctgagg agttttacgt ttacgaagct
      241 ccagccccag caaagttcca gtcatctcaa aattagaact agtgttagac atatatccaa
      301 gcctatctct tattttaaga tacatccact gtctggcgtt tcaaggggcc ggctcaaatc
      361 tatgtacagg ctctcagcat cagcaaagcc tcttactctt ccaactacta ctgttagcat
      421 agtgatcaaa ttcctgatca gatacatgaa ttacctgaac cgaactattt tcatctacaa
      481 atgcatctgg cgatttttct acaggatcat aaagattaaa tccctttcct gtttgaacat
      541 ccaaaaacat aattttttca ccatcgttaa atccagctat ccaatgagtg gcctgttcaa
      601 tagaaatcat tactacactt ccttttgaag tgttttgtaa aatattttct gcaatacgct
      661 cacgaaattt actataagtt tcattaggat gtgacactat gccttcacta tcaatttgct
      721 ctaaatgcct atgaaaaaat ccggtcatat actctgatat ttcattaggt acgggagcct
      781 ctcttactcc ataccctaac atagccatac aagcatgaag cgcacaccct gaacagttac
      841 catccccacc agacggattt atcattttct gtaaagtcgc tgccaattca gaatctttat
      901 ccatatcacc aagactttta gagtttaaag attccagcaa ttttttaaca tttacatttc
      961 cctcctctgt atgtgcctca tatccagcag ttccctgtaa cgacacccca ttaatcatat
     1021 tgtatcctga cagtatatat actcactata ttttcttcat aacaaatatc cctctgatat
     1081 gacaacaaaa aacggagccg gattcctgtt tttgtgaact gcagctattc gagtctaaa
//
LOCUS       DADLSA010000352         1127 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.358, whole genome shotgun sequence.
ACCESSION   DADLSA010000352 DADLSA010000000
VERSION     DADLSA010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.358"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            142..>1127
                     /locus_tag="JN691_004389"
     CDS             142..>1127
                     /locus_tag="JN691_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7245587.1"
                     /translation="MYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAILAGVATTAV
                     WLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDNLQVYSKEQ
                     SDQRYVHREGDTLSGGLTFENDSTLAWIRNTDWAKIGFKNDADSDTDSYMWFETGDNG
                     NEYFKWRHRLAGGRVKDLMNLKWDTLNILVNAVINGCLGIGTTNALGGSSIVLGDNDT
                     GFKQNGDGLLDVYANGQRVFRFQNGSIQSNKAVNVTGRVTPSDYGNFDARYQQRNGGV
                     QDVRYGYEMYYNPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRP"
BASE COUNT          309 a          240 c          308 g          270 t
ORIGIN      
        1 aaaaacagta ttccggacaa ggcgacgcag cagaccagag agagcggtaa tgctgcgtat
       61 gattcaggtt ttcctccggt gaccatgaca gcggtctcag cgggaggtat accgccacac
      121 ggcaaggatt ttaacggtct gatgtacgat attaccgcag caatacggtt cgcccaggct
      181 ggcggtttgt acacgtataa tgccggtttt gcgggggcca ttggtggata tgcaaaagga
      241 gccattctcg ccggagtcgc aacaacagcg gtctggctga ataccacaga cgataacctg
      301 accgatcctg aaggctccga cagtgcgggc tgggtaaatc ttcttgagga tccgaaaagg
      361 atattcctgc ggcagaagaa caatctgtca gaccttcaga ataaagggac ggcacgggat
      421 aatcttcagg tttacagtaa agagcagtca gatcaacgct atgttcatcg ggaaggcgat
      481 accttgtccg gtggacttac ttttgaaaac gactcaaccc ttgcttggat tcgaaatact
      541 gactgggcaa agattggttt taaaaatgat gccgacagcg atactgattc atacatgtgg
      601 tttgaaacag gtgacaacgg caatgagtat ttcaaatgga gacaccgcct cgctggcggc
      661 cgggttaaag acctgatgaa tctcaaatgg gatacactaa atattctggt taatgccgtc
      721 attaatggtt gtcttggaat tggtacgacg aatgcgttag gtggaagttc aatcgtttta
      781 ggagataacg ataccgggtt taaacaaaat ggcgatggtc tgcttgatgt ttatgcgaat
      841 ggacagcgtg tatttcgttt ccagaatggc agtatccaga gtaataaagc tgtaaacgtt
      901 acaggacggg taacaccgtc agactacgga aactttgatg cccgttacca gcagcgaaat
      961 ggtggcgtgc aggatgtgcg gtatggttat gaaatgtatt acaaccctgg cagcaacacc
     1021 gtttcatgga cgtttcgctc accttcggga cacgggctgt cagggatatc gatatcggat
     1081 accggccgta actcagcgga taacgtcaac ggtgtgtatt accgacc
//
LOCUS       DADLSA010000353         1123 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.359, whole genome shotgun sequence.
ACCESSION   DADLSA010000353 DADLSA010000000
VERSION     DADLSA010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.359"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(697..771)
                     /locus_tag="JN691_004390"
     tRNA            complement(697..771)
                     /locus_tag="JN691_004390"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Gly,seq:tcc)
     gene            complement(775..849)
                     /locus_tag="JN691_004391"
     tRNA            complement(775..849)
                     /locus_tag="JN691_004391"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(814..816),aa:Thr,seq:tgt)
     gene            complement(852..928)
                     /locus_tag="JN691_004392"
     tRNA            complement(852..928)
                     /locus_tag="JN691_004392"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(892..894),aa:Arg,seq:tct)
     gene            complement(930..1006)
                     /locus_tag="JN691_004393"
     tRNA            complement(930..1006)
                     /locus_tag="JN691_004393"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(970..972),aa:Ile,seq:cat)
BASE COUNT          320 a          290 c          247 g          266 t
ORIGIN      
        1 ccatctgata catatcccgc ccccccgaca gacctgtgct atcggaaaga aaaaaggcca
       61 tcagcaaaac tctgatggcc tgaatcacct ttaccaatat tgtatgaaaa aacacgcacg
      121 actaattgac aataattttc atttccatta aaaaagatcg cgtagcattc ttaattcatg
      181 aggaacttta cccgccagca atctgagtag cgaaagctgt tcatccccaa tgtttttgct
      241 ggcgggtcct tttttctacg gtcctcctcc ccggaggggg gggcatttca ttatttttct
      301 aatcgttact ggtgagaagg cggaagaaaa agccagttct tcgcccctaa ataacagcat
      361 gattccagtc catgcaaaat ctgatttcca gctcacacat accgagcact gtacaaaaaa
      421 ccgccagcca aggcagttaa gagtgtgttc ccggggtttg cttagaatat tttgtaagtt
      481 gtccgaagag atatttacaa caccagaatg atgcattatc agtcccctgc cagaggttca
      541 ctacgtaacc tgaccgacaa tgcaccattc tggtgctgaa aaaagagcac tggaactgca
      601 acaaaacaac atcacagaac aggagatggt aaggagtcag acattcccac acaatattgt
      661 gtcagtgcaa ataacaacct tcgtctcaaa tctcgctgga gcgggcagcg ggaatcgaac
      721 ccgcatcatc agcttggaag gctgaggtaa tagccattat acgatgcccg catatggtgc
      781 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctctg cctgctgagc
      841 taagtcggcg ctggcccacc accgaggact cgaacctcgc accgtcaact tagaaggttg
      901 atgctctatc cggatgagct agtggtggtt ggtggccctt gctggacttg aaccagcgac
      961 ctggcgatta tgagtcgctc gctctgacca actgagctaa agggccggag gcagaataat
     1021 aaccatatgt catcacatct gcaaactcat ctgaccacca gcgcgtttaa cgccctgtgc
     1081 catttttcag gcacaaaaaa acccgcataa agcgggttct ttc
//
LOCUS       DADLSA010000354         1116 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.360, whole genome shotgun sequence.
ACCESSION   DADLSA010000354 DADLSA010000000
VERSION     DADLSA010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.360"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            28..>1116
                     /locus_tag="JN691_004394"
     CDS             join(28..352,352..>1116)
                     /locus_tag="JN691_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HAY7245588.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRAKKLDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLT"
BASE COUNT          275 a          269 c          338 g          234 t
ORIGIN      
        1 gtttttagac tagtcattgg agaacagatg attgatgtct tagggccgga gaaacgcaga
       61 cggcgtacca cacaggaaaa gatcgcaatt gttcagcaga gctttgaacc ggggatgacg
      121 gtctccctcg ttgcccggca acatggtgta gcagccagcc agttatttct ctggcgtaag
      181 caataccagg aaggaagtct tactgctgtg gccgccggag aacaggttgt tcctgcctct
      241 gaacttgctg ccgccatgaa gcagattaaa gaactccagc gcctgctcgg caagaaaacg
      301 atggaaaatg aactcctcaa agaagccgtt gaatatggac gggcaaaaaa gtggatagcg
      361 cacgcgccct tattgcccgg ggatggggag taagcttagt cagccgttgt ctccgggtgt
      421 cgcgtgcgca gttgcacgtc attctcagac gaaccgatga ctggatggat ggccgccgca
      481 gtcgtcacac tgatgatacg gatgtgcttc tccgtataca ccatgttatc ggagagctgc
      541 ccacgtatgg ttatcgtcgg gtatgggcgc tgcttcgcag acaggcagaa cttgatggta
      601 tgcctgcgat caatgccaaa cgtgtttacc ggatcatgcg ccagaatgcg ctgttgcttg
      661 agcgaaaacc tgctgtaccg ccatcgaaac gggcacatac aggcagagtg gccgtgaaag
      721 aaagcaatca gcgatggtgc tctgacgggt tcgagttctg ctgtgataac ggagagagac
      781 tgcgtgtcac gttcgcgctg gactgctgtg atcgtgaggc actgcactgg gcggtgacta
      841 ccggcggctt caacagtgaa acagtacagg acgtcatgct gggagcggtg gaacgccgct
      901 tcggcaacga tcttccgtcg tctccagtgg agtggctgac ggataatggt tcatgctacc
      961 gggctaatga aacacgccag ttcgcccgga tgttgggact tgaaccgaag aacacggcgg
     1021 tgcggagtcc ggagagtaac ggaatagcag agagcttcgt gaaaacgata aagcgtgact
     1081 acatcagtat catgcccaaa ccagacgggt taacgg
//
LOCUS       DADLSA010000355         1115 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.361, whole genome shotgun sequence.
ACCESSION   DADLSA010000355 DADLSA010000000
VERSION     DADLSA010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.361"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..157)
                     /locus_tag="JN691_004395"
     CDS             complement(<1..157)
                     /locus_tag="JN691_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY7245589.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLAS"
     gene            complement(154..444)
                     /locus_tag="JN691_004396"
     CDS             complement(154..444)
                     /locus_tag="JN691_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY7245590.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(444..1043)
                     /locus_tag="JN691_004397"
     CDS             complement(444..1043)
                     /locus_tag="JN691_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY7245591.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          264 a          298 c          257 g          296 t
ORIGIN      
        1 tggatgccag caactggtta aacatatttc ccgtcttacc gccgccaata tacgaccagc
       61 gcccccacat gcgcagtttt ccctgaatcc agacactttc cagcgtggtg agacgaaggt
      121 gttccccgct tttgcctgta tttgttgggt aaatcataaa taaccttcct ttctccagat
      181 ttcttgtgtg cgaaaaacac cttctgcatg catcaggcgt aattcttctt tggtgtaatc
      241 gctggttttt acccgcccgt cgattaaatc gtggcatgag ctacaggcaa tcgctgcctg
      301 catatcgtgt ggttttgtcg ctgttccgca cgtccccgcc agcctgtaat gcgccagcac
      361 agaagtttcg ggattgtgat tgcagtagcc aggaattctg acggtgcaca tctgcccccg
      421 cgccgcttta cgtaaatcca ccattacgca aactccagta actgcgcggc cacattttcg
      481 acttcctccg gagaggaaaa tttacggaac aggatccagt tccacagcac attcagtaca
      541 gatttataaa cctgctgaaa ctcggtttcg tccatgttcg caaatgcgat agattttgcc
      601 ctgcgcccac gactaccatc aggataaata tgctcggtgt aaaatccggc ctgaatggtt
      661 acccactcgc ggaaagcgtc aaacgacttt agcaatgccg tatcccgggt tctgcatgtc
      721 gcaactgtat taaggtattg ctctgcggca tcactcaggg ctggagtgtg ttcccgacct
      781 actgattcgc acaggtaatc aacgaagcct gataccagtt ttcgttcgcg aggcgtgatc
      841 gccccaccga ccggagtcca gtaatcaaat cccagttgca ggagtttgaa aaaacgcttg
      901 tggaatgcgt agttacgcac acgcttaaag tctgcgtgta tccactcacc tattttgatt
      961 tgatgcaaaa aatcgcaact ctccggcgtc gccgggagaa gtaatccgga agaggtttgt
     1021 ttgaccagtt gtatatgcgc catcgtagtt ctccgctggc gcagtagaat gggtgttcag
     1081 cccgttatgt agtataccag aattaatgcc aatac
//
LOCUS       DADLSA010000356         1114 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.362, whole genome shotgun sequence.
ACCESSION   DADLSA010000356 DADLSA010000000
VERSION     DADLSA010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.362"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..261
                     /locus_tag="JN691_004398"
     CDS             <1..261
                     /locus_tag="JN691_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7245592.1"
                     /translation="DGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQ
                     FKNVPSAFQVGYINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            263..>1114
                     /locus_tag="JN691_004399"
     CDS             263..>1114
                     /locus_tag="JN691_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245593.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEEGNTVVIGYVPRISI
                     PSNGKKGDKIWQSNEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTTVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSH"
BASE COUNT          347 a          200 c          225 g          342 t
ORIGIN      
        1 gatggttcga tccgctttaa caataagatg ccttatcacg ttacgctggg ttatatcgga
       61 acaaacggaa tcacaatgtt gccacaaacg caaagtttaa tggtaacccc ttatagccat
      121 gcagatacgc agtttaaaaa tgttccgtca gcttttcagg ttggttatat caatgatttt
      181 ggtggattaa gtttttatga aataaactgc ccgacggtta ataactcttg taatgtttcc
      241 gtagccaaac gagataaata atatgcgcta cttattaatt gtaattatat ttattatagg
      301 ttttagtgca ttacctgtat gggcaatgga ctgctatgct gaacatgaag agggaaatac
      361 cgtagtcatt ggttacgtac caagaatttc tatccccagt aatggtaaaa aaggtgataa
      421 gatctggcaa agcaatgagt attttatgaa tgtcttctgt aataatgcat tacctgcccc
      481 atcacctgga gaagaatacc catcagcctg gactaatata atgatgtttt taccaggcgg
      541 tcaggacttt tataatcaga actcctatat ttttggcgta acatacaatg gagttgatta
      601 tgattcgact gcccctaatg ctatcgcagc accagaatgc attgatataa aaggagcggg
      661 tacctttaat aatcactata aaaaccccgc tgtctgtagt ggaggtcctg agcctcaatt
      721 atcagtaacc tttccagctc gcgtacaact ctatattaaa ttagcaaaaa atgccaatag
      781 agttaataaa aacctggtgt tgcctgatga atatatcgct ctagagttta aaggtatgag
      841 tggcgcagga gctatagaag tagacaaaaa tttgaccttc aggattcgtg gattaaataa
      901 tattcatgtc cttgactgct ttgttaatgt cgcactggaa ccagctgatg gcgttgttga
      961 ctttggtaaa ataaattccc gaacaattaa aaataccacc gttagtgaga cgtttagtgt
     1021 agtcatgaca aaagatcctg gtgcggcctg tactgagcaa ttcaatattt tagggagctt
     1081 tttcactacg gatattttga gtgattatag ccat
//
LOCUS       DADLSA010000357         1110 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.363, whole genome shotgun sequence.
ACCESSION   DADLSA010000357 DADLSA010000000
VERSION     DADLSA010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.363"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..>1110
                     /locus_tag="JN691_004400"
     rRNA            <1..>1110
                     /locus_tag="JN691_004400"
                     /product="16S ribosomal RNA"
BASE COUNT          278 a          256 c          352 g          224 t
ORIGIN      
        1 cgggaggcag cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg
       61 tgtatgaaga aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt
      121 aatacctttg ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag
      181 ccgcggtaat acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag
      241 gcggtttgtt aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact
      301 ggcaagcttg agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga
      361 gatctggagg aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc
      421 gaaagcgtgg ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg
      481 acttggaggt tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct
      541 ggggagtacg gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg
      601 gagcatgtgg tttaattcga tgcaacgcga agaaccttac ctggtcttga catccacgga
      661 agttttcaga gatgagaatg tgccttcggg aaccgtgaga caggtgctgc atggctgtcg
      721 tcagctcgtg ttgtgaaatg ttgggttaag tcccgcaacg agcgcaaccc ttatcctttg
      781 ttgccagcgg tccggccggg aactcaaagg agactgccag tgataaactg gaggaaggtg
      841 gggatgacgt caagtcatca tggcccttac gaccagggct acacacgtgc tacaatggcg
      901 catacaaaga gaagcgacct cgcgagagca agcggacctc ataaagtgcg tcgtagtccg
      961 gattggagtc tgcaactcga ctccatgaag tcggaatcgc tagtaatcgt ggatcagaat
     1021 gccacggtga atacgttccc gggccttgta cacaccgccc gtcacaccat gggagtgggt
     1081 tgcaaaagaa gtaggtagct taaccttcgg
//
LOCUS       DADLSA010000358         1103 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.364, whole genome shotgun sequence.
ACCESSION   DADLSA010000358 DADLSA010000000
VERSION     DADLSA010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.364"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(139..621)
                     /locus_tag="JN691_004401"
     CDS             complement(139..621)
                     /locus_tag="JN691_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7245594.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(612..914)
                     /locus_tag="JN691_004402"
     CDS             complement(612..914)
                     /locus_tag="JN691_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY7245595.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          321 a          267 c          226 g          289 t
ORIGIN      
        1 tgattttcag gaaccgtttt gacggcttcg gctttaaact ccgtagagta acgttgggtt
       61 tttctgctca ttattggctc cttctgatgc cattctattt caggaaggag tgtccgttaa
      121 actcaggcta cctcagactc agactttata aaacaaggta ttaggtgcca caactgactg
      181 aacaaaacca aaacgctcat aaaatgctct ggcgtcatca tcaattgcat gtaccataac
      241 agccttaatg ccaacctgat cagccagatt gacaacccgg tgaatggcat cacttagtag
      301 ccatttaccg aaaccatgcc cctgggtaca aacatcaaca gccagacgcc ctaacagaac
      361 aacagggaca ggattgggca tgttatgccg caaactgcga ccaagctcgg catgagtgac
      421 ggaaccagta gctaatgagt agtatcccac aacacgtaaa gtatcctcca agcaaataac
      481 gaacgtgcga gaagcattga gcatctggtt tttcatagcc cgaccacgta accagttact
      541 tagcacctca tttccacagt caaatggctg aagtatatgc tcatccgtca acaatgctgg
      601 cgcggtgaca tttatttcca ttcaggcttt accgccatca gacgttcacg cccttctgca
      661 ttctgcacag gagcttccag ttgcgtaata aacgaatcat aacgttcgtt atccagcaca
      721 aataaacgtt gatccaggat aatgttttta gcttcctggt acgccagttc catgatgaaa
      781 tcggtacggt tcttattgac caagctcgca gcataatcaa taacggctcg ttcctcatca
      841 gttgcccgga tgttaatctg atgttctcgc ggtgttttac ggacgcttgc agctgtagac
      901 ataaaaacct ccattggtaa caatttgcta tgcaattaat gttatcgcaa atggtaacag
      961 aacgctatac agaatcactt acgggaaaag cgatcccctg ataaaaacaa tggcagagac
     1021 ctgaacattg tgacgatgat ctgtgtcggc gcacaaatca tgtctgatta cggccgttta
     1081 atgtgatttt gataatgaaa tgc
//
LOCUS       DADLSA010000359         1086 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.365, whole genome shotgun sequence.
ACCESSION   DADLSA010000359 DADLSA010000000
VERSION     DADLSA010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.365"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..>1086
                     /locus_tag="JN691_004403"
     CDS             <1..>1086
                     /locus_tag="JN691_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245596.1"
                     /translation="LSDGKNENGQLFDKQKALKRIKELEGEQIKTARREMVLAVVGTM
                     KAGKSTTINAIVGQEILPNRNRPMTSVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQ
                     EKLATPAGQQVAQTLQQTGDTRELLDILTDDGWLKNEYHGEEEIFTGLASLNDLVRLA
                     AAMGSEFPFDEYAEVQKLPVIDVEFSHLVGMDECQGTLTLVDTPGPNEAGQPQMEVMM
                     RDQLQKASAVLAVMDYTQMNSKADEEVRKELNAIADVSVGRLFVLVNKFDEKDRNGDG
                     ADAVCQKVPAMLNSDVLPASRVYPGSSRQAYLANRALHELRKNGTLPVDEAWVDDFVR
                     EAFGRMKKDYVCKDSELATEGATDLWE"
BASE COUNT          294 a          264 c          307 g          221 t
ORIGIN      
        1 tgttatctga cggtaagaac gaaaacgggc agctttttga taaacagaaa gcactgaaaa
       61 gaattaagga gctggagggc gaacaaatca aaaccgcccg cagggagatg gtgctggctg
      121 ttgtcggcac gatgaaagca ggcaaatcaa ccaccatcaa cgccattgtg gggcaggaaa
      181 ttctgcctaa ccgtaaccgc cccatgacct ctgtaccgac gctcatccgc cacgttcccg
      241 gaaaaactga gccggttctc catctggaac atattcagcc tgtccgcaat ttattaatca
      301 cactgcagga aaaactcgcc accccggcag gacagcaggt cgcacagacc ctgcagcaaa
      361 ccggggatac ccgcgaactg ctggatattc tgacggatga tggctggctc aaaaatgaat
      421 accacgggga ggaggaaatc tttaccggac tggcatcgtt aaacgatctg gttcgtcttg
      481 ctgcggcaat ggggagtgaa tttccttttg atgaatacgc agaagtgcag aaactgccgg
      541 tgatcgacgt ggaattcagc catctggtgg ggatggatga atgccaggga acactcacac
      601 tggtggatac ccccggccct aatgaagccg gacaaccgca gatggaagtg atgatgcggg
      661 atcaactgca gaaagcctct gcggtgctgg cggtgatgga ctacacccag atgaactcga
      721 aagccgatga ggaggttcgt aaagagctta atgccattgc tgatgtgtca gtcggccgcc
      781 tgtttgtgct ggtcaataaa tttgatgaaa aagaccgtaa tggcgatggg gctgatgcag
      841 tctgtcagaa ggtaccggca atgctgaaca gcgatgtgct gcccgcctcc cgcgtttatc
      901 ccggatcctc acgccaggca tacctggcta accgtgcgct tcatgagtta cggaaaaacg
      961 gaacccttcc tgttgatgaa gcctgggtcg atgattttgt cagggaagcc ttcggtcgca
     1021 tgaaaaaaga ttacgtctgt aaagacagcg aactggcaac tgaaggggcg acagacctgt
     1081 gggaag
//
LOCUS       DADLSA010000360         1078 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.366, whole genome shotgun sequence.
ACCESSION   DADLSA010000360 DADLSA010000000
VERSION     DADLSA010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.366"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            1..390
                     /locus_tag="JN691_004404"
                     /pseudo
     CDS             1..390
                     /locus_tag="JN691_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343264.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
     gene            288..710
                     /locus_tag="JN691_004405"
     CDS             288..710
                     /locus_tag="JN691_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY7245597.1"
                     /translation="MRQTEDKQHQNSEPEEGTPCNQTGEDNCPDCGAVMGDATYQETF
                     DEKKPDEAQEEEPKKTEKADDLLPENAGSDQHNDSNNETGEDELNWKKQVLIAAVYGL
                     CANPACIAYAPAIPDIAMMIANKLENFGVTSDERLPDF"
     gene            688..984
                     /locus_tag="JN691_004406"
     CDS             688..984
                     /locus_tag="JN691_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HAY7245598.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
BASE COUNT          348 a          238 c          274 g          218 t
ORIGIN      
        1 aggcaacggc tgaaacagtg gaaccgaatg caactgaaca tcatcagaac acgcagccgc
       61 tggatgctca gtcacaggta aattctgttg atgagaaata tcaggaactg cgggcagaac
      121 tccatgaagc ccggaaaaac attccgctta aaaatcctgt cgatgcagac aaattactgg
      181 ctgcctctcg cggagaattt gttgaaggga ttagcgaccc gaatgattcg aaatggattc
      241 ccggtcatca tatctcctca aatgaagtca aaaaaacgga aaatgaagtg cgacagactg
      301 aagataaaca gcaccagaac agcgaaccgg aggagggaac accctgcaat cagactggcg
      361 aggataactg ccctgactgt ggcgcggtga tgggagacgc aacttatcag gaaacttttg
      421 acgaaaaaaa accagatgaa gcacaggaag aagagccgaa gaaaacggaa aaagctgatg
      481 atttgttacc ggagaacgct ggcagcgatc agcataatga tagcaataat gaaactggcg
      541 aagatgaact caactggaaa aaacaggtgt tgattgcggc ggtttacggt ttatgtgcga
      601 atcctgcgtg catagcctat gcgcctgcaa tccctgacat cgcaatgatg attgccaaca
      661 agcttgaaaa ttttggagtg accagtgatg agcgcttacc cgatttttga tcgtattgaa
      721 gaaatggcat gggctcgcca ctaccagcag atcgttcgtg aagaaaaaga aacggaactg
      781 gcggacgacc tggaaaaagg tctgcctcag cacctgtttg aatcgctctg catcgaccat
      841 ttgcaacgcc acggtgccaa taaacaggca atcagccacg catttgatga tgatgttgag
      901 tttcaggagc gcatggcaga acacatccgg tacatggctg aaaccattgc ccaccaccag
      961 gttgatattg atttggagga ctaaaatcta tggcgctttt tcaacgagca ataaacacac
     1021 aggcctatct aaaagcagga attatggggt ttgctggcga cggcaaaacc tacacagc
//
LOCUS       DADLSA010000361         1040 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.367, whole genome shotgun sequence.
ACCESSION   DADLSA010000361 DADLSA010000000
VERSION     DADLSA010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.367"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            92..274
                     /locus_tag="JN691_004407"
     CDS             92..274
                     /locus_tag="JN691_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HAY7245599.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     RVTELFALLHTEEGCC"
     gene            complement(375..704)
                     /locus_tag="JN691_004408"
     CDS             complement(375..704)
                     /locus_tag="JN691_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAY7245600.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
BASE COUNT          269 a          237 c          238 g          296 t
ORIGIN      
        1 ccgttcagac ggcaggtgcc tgcttttccg gcaacaagca ggaatctcag cgctgaagca
       61 ggaattagag gtaaaaacgc cctaccgggc catgaatcac ccggtcattg gagtagtaac
      121 caaagcagat ttagccagca tggaacaaat atctttggtg aaaagttggt tacgggaagc
      181 tggagcgcac aacgtgttag taaccagtgc agttaataac aatagagtta cagaactttt
      241 tgccttgctg catacagaag aaggctgttg ttaatttcga ttggttctca gatgagaatg
      301 atgacggtga tacacagata agaatcaatg agcaatgagg gttgccgggc aaccctcatt
      361 gaataaaacg ggaattactt cgcttcgccg tttttcattt cgcccatagc tttcagcttt
      421 ttggagatat cgcggcgttc tttggagagc tcggcatttt tgatgatgta atcgtcaacg
      481 cgatcttcat agtcaccttt catgctggca atgatgccct ggattgcttc aacgctcatc
      541 cctggcttga tgtaatcgct caggttgtcc agcaacagga cgcgcttctg gttgtcacgg
      601 atctttttct caacgtcctg aatttcacgt tgcagtttgt tcttacgacg gaacagacga
      661 acaaattcca gtacgtcctg gaatgaaggc ttggtagttt ccatttttat acccctgata
      721 atgtgagagt cggattcgtt taagcaaccg ctattcgtta gggccaacat tactcatagc
      781 tgccgcagat gacaatgctt ttatcccttt tcactatcat accctttatc cttgctgaat
      841 cgaagcagca gcaagatgat tctgaagttc aggaattatt tgcgcaaggc ccggcaaatc
      901 agattcgata gcaactcaag ctgatgtgcc gtttccatgt tcagccacac ataaccatag
      961 attggtgttt ccacgacttt caggtcatgg tggttatcga gcaactgacg cagctcttcc
     1021 acagcatcgt tcaatttttc
//
LOCUS       DADLSA010000362         1035 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.368, whole genome shotgun sequence.
ACCESSION   DADLSA010000362 DADLSA010000000
VERSION     DADLSA010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.368"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..584)
                     /locus_tag="JN691_004409"
     CDS             complement(<1..584)
                     /locus_tag="JN691_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HAY7245601.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPT"
BASE COUNT          308 a          235 c          208 g          284 t
ORIGIN      
        1 gttgggcagg cgtttgcgca ttcaccgcat aacacacatt tcgaggattt tttactttcg
       61 gtatttacgg tggccatcat ccacgggcat gccgtagtac aggcgctaca gccaatacaa
      121 cgtttatgat cgacggtaat acagccttct ttctgctgcc aggtaatcgc accaatcggg
      181 cagacgttca tgcattgcgg ctctttgcat tgacggcagg tgtccgcggt atagttgaga
      241 tcgccataca ggccgccgcc agagccaacc ccgttgtcgc caaagaaata attgcgatgg
      301 attttgatac gggagaagaa tgtccctact gagccatcgt tgaagttggt acaagagatt
      361 tcacagcggt ggcaacccgt gcatcgcgct cgctgggtca ccagcacccc tttgggtgta
      421 tttatcaacc ctactgtgcc gctatcaata tcttcctgtt tgcaacccaa aagtgacagt
      481 aacgcagggg caattgttag accagcaagg ccttttcctg atatacgcaa gaattctaat
      541 cgcgttaagc caatatctaa tagtggacga tcaaccgggt tcatttattt cgcatcctct
      601 tcgcaaacac agtgctgaca attcagcagc ctgatattaa gcattcaact aatacgctgc
      661 tgactctgtc cccaacacca gatattttcc ctaacccgat agtgtatgag aaaagattta
      721 acgccgataa actaatcctt tatggttagg agaaaaaata atgtgattat tctgagccct
      781 taacattgat cgttatcaat taaaattaca aagcaagaaa ttataagcga actgatattt
      841 attatcattt gaaataaatt taacttaagc gacaatcatt tgtgacgttc attgcagaaa
      901 ataaaaataa cattatcgct ataaattaaa atacataccg aaattcgtat aatttcagca
      961 gttgttattt agaatgatta actggaccgc aactgaagca agaatatcag gatgaagctt
     1021 accgcctcat cctga
//
LOCUS       DADLSA010000363         1011 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.369, whole genome shotgun sequence.
ACCESSION   DADLSA010000363 DADLSA010000000
VERSION     DADLSA010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.369"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JN691_004410"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JN691_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HAY7245602.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          292 a          163 c          244 g          312 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc a
//
LOCUS       DADLSA010000364         1006 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.370, whole genome shotgun sequence.
ACCESSION   DADLSA010000364 DADLSA010000000
VERSION     DADLSA010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.370"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(1..992)
                     /locus_tag="JN691_004411"
                     /pseudo
     CDS             complement(1..992)
                     /locus_tag="JN691_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004969972.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT          299 a          262 c          217 g          228 t
ORIGIN      
        1 tcagaaaata gccattaacg ggcgtaccag aagcgttggt acgacataaa ctggctggaa
       61 tatatttgct gtcagcatca cctaatagca ctttaagcgg ggcatcaatc gatgaatacc
      121 agcgcagcta cttgtcccag ggtattcgca atggcgtaaa tcaccattaa agtattaccc
      181 atgttgtaat actcgcccag cttctgaaag gcgtaatatt gaccgttcgt cattaagtca
      241 tccgggatat tacgcgagtc aaacatcatc cccatcgcca gtgagcccag aatggcacaa
      301 accgcaacca tcaccgccag gcatagcatc ccttttggaa attcttttcc tgggttgcgc
      361 gtttgattaa cgtaaggaga aatcttctct gctccgccaa ccgcgaaaac cagcattgaa
      421 atagtggtaa tgtaggtaaa gtcgatatga ggaatgaacg tttcccaggt aatgtttgtg
      481 gtcgcaatat gcacttcagt aatcgcaggc gcggttaccg ccatcgccac atacaggagc
      541 gacataacaa acatcgcaat ccctgccaca gaaccgacga ttttcagcga tttcataccg
      601 cgtgaagcaa cccacataaa gaagataaac agcaccagcg ttaacccctg taacgctacg
      661 actgaatatt ctttgattag cgaaccgtcg cctttcatcg cccaaccgag cgcaatcaga
      721 attgcctggg gtttttgtgc cagataggga atatgcacca cccagtaggt ccacgcggcg
      781 agataagcca gtccgggtcc catcgtgtgt ttaatccagg tactgacccc gcccttccca
      841 tctttgaagg tcgagcctaa ctgaccaaca attagcgcat aaggtgtgaa atagagtgca
      901 aagataaaca cccatgaaaa aacaaccacc agcccctggt tggcatagtt attaacaacg
      961 ttgccaaaac cccagacagt gataaaagac atcagcgcaa tgttgt
//
LOCUS       DADLSA010000365          991 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.371, whole genome shotgun sequence.
ACCESSION   DADLSA010000365 DADLSA010000000
VERSION     DADLSA010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.371"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            81..991
                     /locus_tag="JN691_004412"
                     /pseudo
     CDS             81..991
                     /locus_tag="JN691_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
BASE COUNT          300 a          220 c          232 g          239 t
ORIGIN      
        1 gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt tagttactgg
       61 cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat ggcattaacc
      121 gatatcaaag tgaaaaccgc aaaacccaaa gacaaaccct ataagcttgc tgatggcggc
      181 ggtatgtatc tactgattaa cactaatggt tctaaatact ggcgcatgaa gtaccgcttt
      241 gctggtaaag aaaagatgct ttctattggc gtataccccg atgtaacgct agccgatgct
      301 cgcgagaaac gcagtgaagc caggaaactc ctcgctgctg gaggcgatcc aggggaggca
      361 aaaaaggaag aaaaaatcgc gcagcaaatg agtctgaaaa atacgttcga ggctattgct
      421 cgcgaatggc accagttaaa agctgatcgg tggtcattac gctatcgtga tgaaatcatt
      481 gatacctttg aaaaagacat ttttccctac attggcaagc gccctattgc cgaaatcaaa
      541 ccaatggagt tactggaagc actgcgtaaa atggaaaaac gtggagctct ggagaaaatg
      601 cgcaaggtcc gccagcgctg tggtgaggtg ttccgctatg caattgttac tggccgggca
      661 gactataacc cagcccccga tcttgccagt gctttagcta caccgaaaaa agtacatttt
      721 ccctttctta ctgctaatga acttccccac ttcctcaacg atctggcggg ttataccgga
      781 agtataatca ctaagacagc tactcagatc attatgttaa ccggcgtacg aacacaagaa
      841 ctgcgttttg cacgctggga agatatcgat tttgagacaa aattgtggga gatcccggca
      901 gaagtaatga agatgaagcg acctcatatt gtgccgctct ctgagcaggt cattatgcta
      961 tttaagcagc ttgaaccaat ctcaaaacat c
//
LOCUS       DADLSA010000366          986 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.372, whole genome shotgun sequence.
ACCESSION   DADLSA010000366 DADLSA010000000
VERSION     DADLSA010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.372"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            188..>986
                     /locus_tag="JN691_004413"
     CDS             188..>986
                     /locus_tag="JN691_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7245603.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLG"
BASE COUNT          284 a          204 c          228 g          270 t
ORIGIN      
        1 acaaaaatga aggtactgcc gaaaatattc agatagagct gagggatgac caagatgctg
       61 cgttaaaaaa tggcgatagc aaaacggtta ttcgaggcgc taatcaatgt gatctacacc
      121 tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt taaccttagg
      181 atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc ttctattaat
      241 ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta ttggagcagg
      301 tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga atctggttgt
      361 agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg acactgatca
      421 tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata aaggatcgct
      481 gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg tattggatat
      541 tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag ttggggcggc
      601 cggtggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt ataaaattgc
      661 aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa ataacagtgt
      721 tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcaccgtgg atctcccgga
      781 ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac aaaagctctc
      841 attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta acacagcacc
      901 tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa aaatactggc
      961 tacgggagaa aatgtctccc tgggga
//
LOCUS       DADLSA010000367          983 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.373, whole genome shotgun sequence.
ACCESSION   DADLSA010000367 DADLSA010000000
VERSION     DADLSA010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.373"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JN691_004414"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JN691_004414"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAY7245604.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          260 a          220 c          221 g          282 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcggaatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgaaaaa aaacctcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DADLSA010000368          981 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.374, whole genome shotgun sequence.
ACCESSION   DADLSA010000368 DADLSA010000000
VERSION     DADLSA010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.374"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            194..981
                     /locus_tag="JN691_004415"
                     /pseudo
     CDS             194..981
                     /locus_tag="JN691_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT          325 a          209 c          170 g          277 t
ORIGIN      
        1 ggttgaacta tatccctgct tttcaaccat cctaaaaaag catgaattgc tcgctctacc
       61 ctaagtggct gtgctatttt agaaaaactt aatcctgagt taagcaaaaa aacataatca
      121 ataaaaatac atacgcaaaa aacattgatt aagtgaatat accatggaag aaaaatataa
      181 accggagtag tgtatggcca atctctacga cttgaaaaag ttcgacttaa atcttcttgt
      241 catatttgag tgtatttacc agcatttaag cattagcaaa gctgctgaat cactatatat
      301 caccccatca gccgtgagcc agtcgttaca acgcttacgt gcgcaattta atgacccttt
      361 attcattcgc tcaggaaaag gtatcgcgcc gacgacaaca gggttgaatc tgcatcacca
      421 tcttgagaaa acctcaaagg ccttgaacaa acaattaata tagtcaataa gtccgaacta
      481 aagaaaaact ttattatcta cggccctcag ctcatttctt gctctaataa tagtatgctg
      541 atccgctgcc tacggcaaga tgcctctgtc gagattgagt gccatgatat cctcatgtca
      601 gcagagaatg ctgaagaact gttggttcac cgcaaagccg atctggtgat cacccaaatg
      661 ccagtgatca gccgttccgt catttgcatg ccattgcata caattcgcaa tactctaatt
      721 tgtagtaata agcatccgcg aattactgac aatagcactt atgaacaaat tatggcagaa
      781 gagttcaccc aattaatatc caaatcagca ggagttgatg attttcaaat ggaaatagac
      841 gagaagttta tgaatcgcaa aatttcattt cgtggttcct ctttgctgac aataattaat
      901 agcattgcag ttacagattt actgggaata gtcccctatg aactttataa ctcccaccgt
      961 gactttctta atttaaaaga g
//
LOCUS       DADLSA010000369          979 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.375, whole genome shotgun sequence.
ACCESSION   DADLSA010000369 DADLSA010000000
VERSION     DADLSA010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.375"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(36..551)
                     /gene="sfmF"
                     /locus_tag="JN691_004416"
     CDS             complement(36..551)
                     /gene="sfmF"
                     /locus_tag="JN691_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HAY7245605.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(562..>979)
                     /locus_tag="JN691_004417"
     CDS             complement(562..>979)
                     /locus_tag="JN691_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7245606.1"
                     /translation="NCELNAGQIVEFDFGDIGASLFSAAGPGNRPAGVMPQTKSIAVK
                     CTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIVADQNDTPITPNDLNSVIPFRLDA
                     AAAANVTLRAWPISITGQKPTEGPFSALGYLRVDYQ"
BASE COUNT          258 a          266 c          220 g          235 t
ORIGIN      
        1 atcgactacg ccaaattata cgaattaata taatattaat tataattgat cataaatatc
       61 gcatccgctt tcgccacacc tggccgaaca ccgctggcta acgctcgata attggcaaaa
      121 aagtttagtg tgacattgcc gtttgcatct acttcctcag tcgggctcgc ctcccccagt
      181 gcgagccggg agcgatcgct attacgtaat tcgatggcga cggtttgcgc cattgcggga
      241 tcatccagag ccagcaggtt ggtatcggat gccggcgttc ccgtaaataa aatcgcaact
      301 gaacccggag gacatccctc cagccgcagg ctaaaaggga cgagtgccgt ggtatcgcca
      361 gcgttcagta attgtgtcgt aggccatctg cctaaatcta ccgtcttatc aatatccgct
      421 gtgtttacgg tacaggagaa atcaacaacg ttaccgtgca aattgatatt aatcgttcct
      481 aaagggtcag ctgcccatcc actggaactc cacagtaacc cgcagaaaca gctaaagaat
      541 actcttctca ttctccgtac ctcattgata atcgacgcgt aaatacccca gcgcgctaaa
      601 cggcccttcg gtcggttttt gaccggtaat actgataggc caggcgcgaa gtgtgacatt
      661 ggctgccgca gctgcatcca gacggaaagg aataacgcta ttgagatcgt taggcgtgat
      721 cggcgtatcg ttctgatcgg cgacaataaa acctaaatcc tgattgtccg acaccatcgc
      781 ctgaccagaa acggcactgg cttccagacg cattgttaaa taagcctgcg cagcaacatt
      841 cgtacatttg accgcaatgc tcttggtttg cggcatgacg ccagctggtc gattccccgg
      901 ccctgccgca ctaaataacg atgcgccgat atcaccaaaa tcaaattcaa caatctgccc
      961 ggcatttaat tcgcagttt
//
LOCUS       DADLSA010000370          956 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.376, whole genome shotgun sequence.
ACCESSION   DADLSA010000370 DADLSA010000000
VERSION     DADLSA010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.376"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(304..747)
                     /locus_tag="JN691_004418"
     CDS             complement(304..747)
                     /locus_tag="JN691_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245607.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLPRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCVAAVVRTALRAVRDTVVSVARAVWNWIFFLTR"
BASE COUNT          253 a          231 c          253 g          219 t
ORIGIN      
        1 ccggctcatc aaccatttgc cggagcagat tgatgctcag ttgcaaaagc cggtcggctt
       61 catcacaaag cagggtgatg ttcttttcgt gcattattta accttcctga gacgcatttt
      121 catgacaacc acggaggagg ccatttacta caaacgatcg tttatagcga tcaatataat
      181 agcaacgatc gttatattct attaatatga aaaacacaac gcaaggggga caacccgggt
      241 atggctgcgt ttgcattgtg ttttcataga gttaaaaatc tgtaacgaca acccggcaca
      301 tcactaccgg gttaagaaga aaatccagtt ccataccgcg cgggccacag agaccacggt
      361 gtcacgaacg gcgcgcaggg ccgtacggac aacagcggca acacagacac tctccgccgt
      421 gtcaaatatc cggtccaccg caccggtaaa ctgttcacgg gtctgagtgc ggacagactc
      481 cgtgcgcagt tcgtcctgca ggcgtgtcat cagagggatg gcggcatgtc cgggaagcac
      541 tgtcatgagt gcactgaccg gggtgacctc cccctgaaac agtgcattac agagtgagga
      601 tttaccggcc ccgcttttac ccataatgcc aggcacgggt tcgtgacggg ggagtttgcg
      661 cagatgttcc aggatgtgac gggaaatcgg gtaaggcagg gaggagagcg gtttttcaat
      721 tgcctcaatg gcatcagacg gattcataca tgtattccca gtgaaaaaag acaaaacccc
      781 gtgcaccgga aaggtggcgg gggattcacg gagataatat ctttctgaaa ttattttgaa
      841 tgcaggtata tgtggctgat ttgtatcagc taatgagtac aggtctgaat ctaatctgac
      901 agtccgcttt gtgccaggag cggactgacc tgctccccga tgattaatac acagct
//
LOCUS       DADLSA010000371          927 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.377, whole genome shotgun sequence.
ACCESSION   DADLSA010000371 DADLSA010000000
VERSION     DADLSA010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.377"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..97)
                     /locus_tag="JN691_004419"
     CDS             complement(<1..97)
                     /locus_tag="JN691_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245608.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNE"
     gene            236..907
                     /gene="queE"
                     /locus_tag="JN691_004420"
     CDS             236..907
                     /gene="queE"
                     /locus_tag="JN691_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAY7245609.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          261 a          193 c          219 g          254 t
ORIGIN      
        1 attcgttatc attaaaaact aatacagaaa aaataaatat aattttggtg aatgttttga
       61 catgattaaa tccattttct ttattttctt ctgacataat acgttactcc ctttaattat
      121 taacaatatg tttcgtattt ttctttaaat ctgcatgtaa cagaatgtta ataagacatg
      181 attgttgcgg caggatctgc aataattaac aattatccac ttcacagaga atgctatgca
      241 gtacccgatt aacgagatgt tccagaccct gcaaggtgag ggttacttta ccggcgtccc
      301 cgccattttt attcgtttac agggatgccc ggttggctgt gcctggtgcg acaccaaaca
      361 cacctgggaa aagcttgagg atcgggaagt ctcccttttc agcattctgg cgaagaccaa
      421 agagagtgat aagtgggggg cggcgagcag tgaagacttg ctggctgtca ttggtcgtca
      481 gggatatacc gcgcggcatg tggtgattac gggtggtgag ccttgcattc atgatttgct
      541 gccactgact gacctgctcg aaaagaacgg ttttagctgc cagatcgaaa ccagtggaac
      601 tcatgaagta cgctgcacac cgaatacctg ggttaccgta tcgccaaagc tgaacatgcg
      661 cggcggctat gaagtgttgt cacaggcact ggagcgagcc aacgaaatca agcatccagt
      721 ggggcgcgta cgcgatattg aagcactgga tgaactactg gcaacgttga ccgatgataa
      781 accgcgagtc attgcactgc agccgattag ccaaaaggat gatgccacac gattgtgcat
      841 tgaaacctgt attgcgcgta attggcgttt gtcgatgcaa acacataaat atttaaatat
      901 tgcctgatta cacatttata agcgtta
//
LOCUS       DADLSA010000372          921 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.378, whole genome shotgun sequence.
ACCESSION   DADLSA010000372 DADLSA010000000
VERSION     DADLSA010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.378"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..>921
                     /locus_tag="JN691_004421"
     rRNA            <1..>921
                     /locus_tag="JN691_004421"
                     /product="23S ribosomal RNA"
BASE COUNT          256 a          205 c          288 g          172 t
ORIGIN      
        1 accgagattc ccccagtagc ggcgagcgaa cggggagcag cccagagcct gaatcagtgt
       61 gtgtgttagt ggaagcgtct ggaaaggcgc gcgatacagg gtgacagccc cgtacacaaa
      121 aatgcacatg ctgtgagctc gatgagtagg gcgggacacg tggtatcctg tctgaatatg
      181 gggggaccat cctccaaggc taaatactcc tgactgaccg atagtgaacc agtaccgtga
      241 gggaaaggcg aaaagaaccc cggcgagggg agtgaaaaag aacctgaaac cgtgtacgta
      301 caagcagtgg gagcacgctt aggcgtgtga ctgcgtacct tttgtataat gggtcagcga
      361 cttatattct gtagcaaggt taaccgaata ggggagccga agggaaaccg agtcttaact
      421 gggcgttaag ttgcagggta tagacccgaa acccggtgat ctagccatgg gcaggttgaa
      481 ggttgggtaa cactaactgg aggaccgaac cgactaatgt tgaaaaatta gcggatgact
      541 tgtggctggg ggtgaaaggc caatcaaacc gggagatagc tggttctccc cgaaagctat
      601 ttaggtagcg cctcgtgaat tcatctccgg gggtagagca ctgtttcggc aagggggtca
      661 tcccgactta ccaacccgat gcaaactgcg aataccggag aatgttatca cgggagacac
      721 acggcgggtg ctaacgtccg tcgtgaagag ggaaacaacc cagaccgcca gctaaggtcc
      781 caaagtcatg gttaagtggg aaacgatgtg ggaaggccca gacagccagg atgttggctt
      841 agaagcagcc atcatttaaa gaaagcgtaa tagctcactg gtcgagtcgg cctgcgcgga
      901 agatgtaacg gggctaaacc a
//
LOCUS       DADLSA010000373          910 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.379, whole genome shotgun sequence.
ACCESSION   DADLSA010000373 DADLSA010000000
VERSION     DADLSA010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.379"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            157..821
                     /locus_tag="JN691_004422"
                     /pseudo
     CDS             157..821
                     /locus_tag="JN691_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
BASE COUNT          287 a          192 c          199 g          232 t
ORIGIN      
        1 aaagcttatt ttcagcttta attaactaac cacgtcattg ttctgctgct aataacaaca
       61 aaggtgggtg gtttatgatt attcacttta atacaccagg tgaactcctt ctgccatgca
      121 ggcagggttg gacagaaaac acgcgaagga attaaaatgc ctggaacggg aaaaatgaaa
      181 catgtcagtt tgactctgca ggttgagaac gacctgaaac atcagcttag tattggcgca
      241 ttaaaacctg gcgcacgcct gattactaaa aatctggcgg agcaattagg tatgagtatt
      301 acacctgtgc gtgaagcatt attacgtctg gtttcggtga atgcgctttc tgtcgcacct
      361 gcacaagcat ttacagtacc ggaagtgagg caaagtcagt tagacgaaat caatcggatc
      421 cgctacgaac tggaattaat ggcagttgcc ctggctgttg aaaacctctc ctcgcaagac
      481 atagcagaac ttcaggaatt gctcgggaaa ttacagcagg cgcaagaaaa gggcgacatg
      541 gaacaaatca ttaatgcgaa caggctattt cgcttagcga tttatcatcg ctcaaatatg
      601 cccatcctgt gtgagatgat tgagcaactg tggatcagga tgggacctgc tttacattat
      661 ctttatgaag cgattaaccc agcgtaatta cgggagcgca tagaaaacta tcatctatta
      721 ctcgctgcgt taaaagcaaa agacaaagag ggatgcaaac attgtcttgc tgaaattatg
      781 caacaaatat tgctatttta taccagcaat acaatcgtta aattaaatgc ccggcaaata
      841 ccgggcatca agtaattact caaatcgcca cgcaatattc atgccgacac cataatttcg
      901 tccgggcgca
//
LOCUS       DADLSA010000374          906 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.380, whole genome shotgun sequence.
ACCESSION   DADLSA010000374 DADLSA010000000
VERSION     DADLSA010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.380"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            62..>906
                     /locus_tag="JN691_004423"
     CDS             62..>906
                     /locus_tag="JN691_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY7245610.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATVALINGASLTLTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIK"
BASE COUNT          269 a          190 c          207 g          240 t
ORIGIN      
        1 agacagacat agcattatgt gatctactga tttgtgccaa caattcaatg agatcactgc
       61 tatgcctgtt cataaagtat gtcagaattt cttccgggat gccttagcac catttcatca
      121 gtaccgacaa aatgccctga tggatgccac agtggcgtta atcaacggag catctctgac
      181 gctgaccagt atcggacgtt ttttgccggg taatgcacaa gttaagaata agatcaaacg
      241 tattgatcgc ctcatgggta atgaggcgct ccatcgcgat attccaatga ttttcaggaa
      301 tatcacttca atgctgacac ggcagttatc tttatgtgta atagccgtag actggagtgg
      361 ctatccctcc caggagcatc atgttcttcg tgccagcctt ctgtgtgacg ggcgttccat
      421 tccgttattg agtaaggttg ttccttctga aaaacaaaat aatccattga tacaacatga
      481 tttccttgat tcccttgctc aatccctgcc ccccgatgcc agggttatta ttgtcacgga
      541 tgctggattt cagagcgcat ggtttcacca tattacttct ctgggctggg attttattgg
      601 tcggatcaga aataacgttc agtattgcct cgataacgcg cctgaaagat ggttaaaagt
      661 gtcggatagc cctgaatgta aaacaccgga atatatgggg gctggaaggc tggttaaaga
      721 aaggaaaaaa agcatccggg ggcattttta cacctataag aaatcggcaa aagggcgaaa
      781 aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca gacaaagaac agagcaaatc
      841 agcaaaagaa gcctggttga tcttcagcag tacaaatgac ttcagagcgc gagaaatcat
      901 caagtt
//
LOCUS       DADLSA010000375          890 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.381, whole genome shotgun sequence.
ACCESSION   DADLSA010000375 DADLSA010000000
VERSION     DADLSA010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.381"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            83..>890
                     /locus_tag="JN691_004424"
     CDS             83..>890
                     /locus_tag="JN691_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HAY7245611.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSR"
BASE COUNT          239 a          233 c          240 g          178 t
ORIGIN      
        1 agatcacttt gagggaactt agcccggatt gtgcgatctg atcaatcgcc aaatcaaaac
       61 aaatcaccaa ccggactgag caatgccgat catagcacca atttcccgtg acgaacgacg
      121 cctgatgcag aaagccatcc ataaaacaca cgataaaaat tatgcccgca gactgactgc
      181 catgctgatg ctgcaccggg gcgaccgtgt cagcgacgtt gccagaacgc tctgctgcgc
      241 ccgctcctct gttggacgct ggattaactg gttcacgcag tcgggtgttg agggactgaa
      301 atcattacct gccgggcgtg cccgtcgctg gccgtttgag catatctgca cactgttacg
      361 tgagctggta aaacattctc ccggcgactt tggctaccag cgttcacgct ggagtacaga
      421 actgctggca ataaaaatca atgagataac cggttgccag ttaaatgccg gaaccgttcg
      481 ccgctggttg ccgtctgcgg ggattgtgtg gcgaagggct gcgccaactc tgcgtatccg
      541 tgacccgcat aaagatgaaa agatggcagc aatccataaa gcactggacg aatgcagcgc
      601 agaacatccg gtcttttatg aagatgaagt ggatatccat cttaatccca aaatcggtgc
      661 ggactggcaa ctgcgcggac agcaaaaacg ggtggtcacg ccgggacaga atgaaaaata
      721 ttatctggcc ggagcgctgc acagcgggac aggtaaagtc agctatgtgg gcggcaacag
      781 caaaagttcg gcgctgttca tcagcctgct gaagcggctt aaagcgacat accgtcgggc
      841 gaaaaccatc acgctgatcg tggacaacta cattatccac aaaagccggg
//
LOCUS       DADLSA010000376          874 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.382, whole genome shotgun sequence.
ACCESSION   DADLSA010000376 DADLSA010000000
VERSION     DADLSA010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.382"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..226)
                     /locus_tag="JN691_004425"
     CDS             complement(<1..226)
                     /locus_tag="JN691_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245612.1"
                     /translation="MKWLKLIFWGLCGLQICQLSYAQEALTEKYSQSELLRNWALSHC
                     LSLVYKNDAVQDDARATASAYLEYGQQPVEI"
     gene            complement(223..709)
                     /locus_tag="JN691_004426"
                     /pseudo
     CDS             complement(223..709)
                     /locus_tag="JN691_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459275.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(762..>874)
                     /locus_tag="JN691_004427"
     CDS             complement(762..>874)
                     /locus_tag="JN691_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245613.1"
                     /translation="GYYDAKFFLRPHFQLEGLTGSLRLVTKLPSVKQGNA"
BASE COUNT          281 a          208 c          144 g          241 t
ORIGIN      
        1 aaatttctac aggttgctga ccatattcaa gataagcact agctgtagct ctggcatcat
       61 cctgaacggc atcattttta tacaccaatg acaaacaatg actcaaagcc caattgcgta
      121 agagctctga ctgagaatat ttttcagtca atgcttcctg agcataagat aattgacata
      181 tctgtagccc acagaggccc caaaatatta atttcagcca tttcatggta gtacccacag
      241 gattccgact tcaggaacaa atgttccatt atctggatca tttagtagat gacattgatc
      301 tgaacaaacg ctaccatccc ataatgttac atgtccccga gcattgctcc agccatggcc
      361 ttttacaaca atgatgcctt ttttacctgt aaagtcacca ggagttgtga ttattaatga
      421 caagatctgg ctcccccata atctgggtca gatgttcaat catatcattc acacgataaa
      481 tataaaattt cttatcagaa ccactaaccg ttgcataggg agaactacgg gatatcggaa
      541 atcccgtagc attcaaaaca taactcatac gaattggaca agcattttca aaatagccac
      601 ctgaaatatt ttgcccaaca tttccgccaa ttatacgacc gacatcagca acgggaaaat
      661 tcacttttcc aaatgccgac catgcctcat taaatgaagg tctacccatt ttctttaccc
      721 tctaaaaaat ctgcatcaca agctttatga aatataataa atcaggcatt accctgtttt
      781 accgacggca gttttgtgac cagacgcagt gatcctgtta acccttcaag ctgaaaatgc
      841 gggcgcagga agaatttagc atcgtaataa cccg
//
LOCUS       DADLSA010000377          842 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.383, whole genome shotgun sequence.
ACCESSION   DADLSA010000377 DADLSA010000000
VERSION     DADLSA010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.383"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(94..459)
                     /locus_tag="JN691_004428"
                     /pseudo
     CDS             complement(94..459)
                     /locus_tag="JN691_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            complement(732..>842)
                     /locus_tag="JN691_004429"
     CDS             complement(732..>842)
                     /locus_tag="JN691_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY7245614.1"
                     /translation="FRTCCSRVDVWLNLAEYMLYLPMRDAFSSGDLKTVC"
BASE COUNT          221 a          201 c          216 g          204 t
ORIGIN      
        1 atcatcatcc cgtaactgaa ttgtggaaac agtgacaccg cctctctgat aacagagaac
       61 tcgcgtgtcg taacttttct ggcatgaaac tggcgttgac ggatcctttt gactgaaata
      121 gcagtcttcc agtttttcga acacatccca cgcctgatcg gtttcgagca ttttggcgtg
      181 gcgtgctgcg cctcgttctg tccagaggat gagggagcgg gctttgggtg aaactggatt
      241 ttgtgagtag tttaaagcga cccgcaattc tttaaggtca ttaccaacaa ctttgaaaaa
      301 gtgtttccct tcaacgaagc gtactttgtt ctcatgatga ttctggcgaa tacgcaccgg
      361 ctcagtgccg taaagctgcg ccaaaagttc ggtggtaata acaggaatct ggttatgggt
      421 gatcggggag agagtttcaa cagagatttg agtggtcata acgataactc cgtacatttg
      481 gaaattatcg ccaccgacga cgccaatcga actggtggcg aactgtgcag ggttggcgta
      541 accgggtacg gaaaccggcg agcctttcgg ctcccccaca cagcccgcca taaatcgcga
      601 atgtgactat gcaaacgata tgaaaaaaga cgcgggcgcg tctcatatcg ctccgtaaac
      661 atccggggtg ctaatcccga cgccagattt tgctggcgcg tgaggaatat agccccggat
      721 aacagattga gtcaacaaac ggtttttaga tccccggaag agaatgcatc acgcatcggt
      781 agatagagca tatattcagc aagatttagc catacatcta cccgactgca gcacgtccgg
      841 aa
//
LOCUS       DADLSA010000378          823 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.384, whole genome shotgun sequence.
ACCESSION   DADLSA010000378 DADLSA010000000
VERSION     DADLSA010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.384"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            29..>823
                     /gene="ltrA"
                     /locus_tag="JN691_004430"
     CDS             29..>823
                     /gene="ltrA"
                     /locus_tag="JN691_004430"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY7245615.1"
                     /translation="MKDRNGSGAKGLPHCADGAAATTGDNADGRTAVKSAKPFPVSKR
                     QVWEAYKRVKANRGAAGIDGQTLAGFDENVTDNLYKLWNRMASGSYMPQAVRRVDIPK
                     ADGGVRPLGIPAVSDRIAQMVVKQILEPVLEPLFHADSYGYRPGKSAHQAIAQARKRC
                     WKFDWVVEVDIKGFFDDIDHHLLLKTVQHHTQARWVVMYIERRLKAPVQMPDGAMLAR
                     GRGTPQGGVISPLLSNLFLHYAFDMWMQRQFPGVPFERYADDVVCHC"
BASE COUNT          195 a          197 c          256 g          175 t
ORIGIN      
        1 tacagggacg gagtaaaccg tagtagcggt gaaggaccgt aatgggtccg gagcaaaggg
       61 cttgccccat tgtgctgatg gtgcggcggc aaccacgggc gacaatgccg atgggaggac
      121 tgctgtaaaa tcggcaaagc catttcctgt aagtaaacga caggtatggg aagcttataa
      181 acgagtaaaa gccaaccgtg gggcggcagg cattgacggg cagacactgg ccgggtttga
      241 cgagaatgtt acagacaatc tctacaaact gtggaacaga atggcttccg gcagttatat
      301 gccgcaggcg gtaaggcgtg tggacattcc caaagctgac ggtggtgtac gtccgctcgg
      361 tattccggcc gtgtcggacc ggatcgccca gatggtcgtt aagcagatac tcgagccggt
      421 actggaaccc ctgttccatg cagactcgta tggttatcga ccgggtaaat ccgcacacca
      481 ggcgattgcg caggcgagga aacgctgctg gaagtttgac tgggtggtgg aagtcgatat
      541 taaaggcttc tttgatgata tcgatcatca cctgctactg aagacagtgc agcatcatac
      601 tcaggcacgc tgggtggtga tgtacattga gcgcaggctg aaggcaccgg tgcaaatgcc
      661 ggatggagca atgctggcac ggggtcgagg aacgccgcag ggcggcgtaa tctcccccct
      721 gctctcaaat ctgttcctgc actatgcctt tgatatgtgg atgcaacgtc agtttcccgg
      781 cgttcccttc gaaaggtacg ccgatgatgt tgtatgccac tgt
//
LOCUS       DADLSA010000379          819 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.385, whole genome shotgun sequence.
ACCESSION   DADLSA010000379 DADLSA010000000
VERSION     DADLSA010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.385"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..153
                     /locus_tag="JN691_004431"
     CDS             <1..153
                     /locus_tag="JN691_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245616.1"
                     /translation="YWFLQLLDRVSPSQWAAIGVLGSLLFGLLTYLTNLYFKIREDRR
                     KAARGE"
     gene            158..502
                     /locus_tag="JN691_004432"
     CDS             158..502
                     /locus_tag="JN691_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HAY7245617.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARMSIRQVFLDVAAGLCEGDEQSPEKRPIILEAQNVWITYKGKLPG
                     RITDSLKTPPKW"
     gene            complement(468..740)
                     /locus_tag="JN691_004433"
     CDS             complement(468..740)
                     /locus_tag="JN691_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245618.1"
                     /translation="MDNNTISLQELLDCISRLRDDVNALTVAFSSLALSIPREQMQPT
                     LASLLLESRNPKWSQEQQNSFKWLAALLEEKYAGKITISVESSENQ"
BASE COUNT          217 a          164 c          220 g          218 t
ORIGIN      
        1 tactggttcc tgcagttgct ggacagggtt tccccgtctc agtgggcggc aataggcgtg
       61 ctggggagtc tgctgtttgg gctgctgaca tatctgacta acctgtattt caaaatcaga
      121 gaggaccgtc gtaaggctgc acggggagag taattcaatg actcaaaact atgaactgat
      181 tgtgaaaggg atccgcaatt ttgagaataa agttacggta actttagcgt tacgggacaa
      241 aaaacgcttt gacggtgaaa tttttgacct ggacatctcg ctggaccgtg ttgaaggtgc
      301 cgcgctggag ttttatgagg cagcagccag aatgagcatc agacaggtct tcctggatgt
      361 tgctgccggg ttatgtgaag gggatgagca gtcgccggaa aagcgcccca taattttaga
      421 ggcgcagaat gtgtggataa cctacaaagg aaagctacca ggaagaatta ctgattctct
      481 gaagactcca ccgaaatggt aattttacca gcatattttt cttccagtaa tgccgccagc
      541 cacttgaaag aattttgttg ttcctgggac catttggggt tgcgtgattc aagcaggagc
      601 gatgccagtg ttggttgcat ttgttctctg ggaattgata aggccagaga tgaaaatgca
      661 acagtgaggg catttacatc atcccgaagc ctggaaatgc agtcgagcaa ctcctgtaga
      721 gaaatggtgt tattgtccat aaataatcct cttgattgtc tttacctttt cccagcctga
      781 ttcaacaggc tgggacagat aaacatatcc agggttcag
//
LOCUS       DADLSA010000380          799 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.386, whole genome shotgun sequence.
ACCESSION   DADLSA010000380 DADLSA010000000
VERSION     DADLSA010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.386"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(165..677)
                     /locus_tag="JN691_004434"
     CDS             complement(165..677)
                     /locus_tag="JN691_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7245619.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
BASE COUNT          242 a          166 c          182 g          209 t
ORIGIN      
        1 ggacggaatc atcagcgacc agacagcatt gagaatgctt caggagtaca tcaggatcca
       61 atgccttggg gggtagcggt aattttactc attatccttc aaatcagatt ctgttgtcag
      121 aggaatgggg gaagctggat tcgcaagagc tttttatggg cagtttagct aaaaaaaggc
      181 aacacgaagc taaaactacc aacataaagt gctgaccagc tagcagctga aatcagactc
      241 actgtataca ctttcactgg atgaagttgc aacattcctg ccaccaaagg agcaatgtaa
      301 cgcagcacgg cgataaagcg cgaagtgaaa agtattgaca cagagttatt ttgcaactga
      361 aggcgaaccc gttctattgt attggaacgg ctcgatatta tacatgcaag ttgagggata
      421 tgcccaatta ttataccaag atggtaatta acgatagtac cgcaccatgc gccactcatg
      481 accgtgattc cagcttccca tggtgaaaga gtcgtcttgc tgactgtaat tacagtggcc
      541 atcataattg atgctggtgg caatatcgcc gaaatgagta aagtggattt tgtgaacgct
      601 attataaaaa gaagccccca taggcgatat ggatgaagta caaaatagtt catcaaagca
      661 tttatccact ccatcaatgt ctttccttct gtgaattttt ctttgctatt taaccgtgat
      721 acgacttaat ttaggcttaa acaccttaca tttacaaggt actcctgtgg ggggccctgc
      781 cacggagcgt cgcaggagt
//
LOCUS       DADLSA010000381          780 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.387, whole genome shotgun sequence.
ACCESSION   DADLSA010000381 DADLSA010000000
VERSION     DADLSA010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.387"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..>780
                     /locus_tag="JN691_004435"
     CDS             <1..>780
                     /locus_tag="JN691_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY7245620.1"
                     /translation="RQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAE
                     QLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHL
                     VKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHR
                     WEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYW
                     PNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQL"
BASE COUNT          226 a          177 c          231 g          146 t
ORIGIN      
        1 cccgccagac acatgccggg aacccggcgc tctacccgca ggtcaaaatg gtctgccaga
       61 tggaactgac cagccatctg ctgacggctg cagccttcgg cacgatgaag aacagcgaaa
      121 atgagcttgc tgagcaactt atagaacaaa ccggcgataa cactctgacg ttaatggata
      181 aaggttatta ctcactggga ctgttaaatg cctggagcct ggcgggagaa caccgccact
      241 ggatgatacc tctcagaaag ggagcgcaat atgaagagct cagaaaactg ggtaaaggcg
      301 atcatctggt gaagctgaaa accagcccgc aggcacgaaa aaagtggccg ggactgggaa
      361 atgaagtgac agcccgcctg ctgaccgtga cgcgcaaagg aaaagtctgc catctgctga
      421 cgtcgatgac ggacgccatg cgcttccccg gaggagaaat ggcggatctg tacagtcatc
      481 gctgggaaat cgaactggga tacagggaga taaaacagac gatgcaactg agcaggctga
      541 cgctgagaag taaaaagccg gagcttgtgg agcaagagct gtggggtgtc ttactggctt
      601 ataatctggt gagatatcag atgattaaaa tggcggaaca tctgaaaggt tactggccga
      661 atcaactgag tttctcagaa tcatgcggaa tggtgatgag aatgctgatg acattgcagg
      721 gcgcttcacc gggacgtata ccggagctga tgcgcgatct tgcaagtatg ggacaacttg
//
LOCUS       DADLSA010000382          763 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.388, whole genome shotgun sequence.
ACCESSION   DADLSA010000382 DADLSA010000000
VERSION     DADLSA010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.388"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..>763
                     /locus_tag="JN691_004436"
     CDS             <1..>763
                     /locus_tag="JN691_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7245621.1"
                     /translation="YLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLK
                     GIVIKDEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIK
                     MADGTLNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMVEVNLYKVIAPYVNLFK
                     ADLSFSKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNM
                     ANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFD"
BASE COUNT          252 a          133 c          120 g          258 t
ORIGIN      
        1 ttaccttagt aaagaaaagg atgtatccat taaggtggta tattttataa ataatgtcgc
       61 cgtccataat aatactatcg aaattccaca gatagtaaat ggtggttacg atttttcaca
      121 ccttagcctg aagggtatcg tgattaaaga tgaagattta tccaattcga attttgcagg
      181 ttgcagacta caaaacgcta ttttccagga ctgtaatatg tataaaacga atttttatta
      241 tgccataatg gaaaaaatac tttttgataa ttgtattctc gatgactcaa atttcgctca
      301 gataaaaatg gccgacggaa ctctaaatgc atgctccgct atgcatgttc aattctacaa
      361 tgcagcaatg aatagagcca atattaaaaa taccttcctt gactattcaa atttttatat
      421 ggcgtacatg gttgaggtaa atctttataa agtaatagcg ccatatgtta atttatttaa
      481 agccgacctt agtttctcta aactcgattt aattaacttt gaacatgctg atctgtctcg
      541 cgtcaatctg aacaaagcaa tcctccagaa tataaactta attgatagca aactcttttg
      601 tacgtggctg acaaatacat tcctcgaaat ggttatatgt accggctcta atatggctaa
      661 tgttaatttt aataatgcca atttaagcaa ctgccatttc aactgttcta ttttaacaaa
      721 agcctgtatg tttaataccc gtctctatcg ggttaatttc gat
//
LOCUS       DADLSA010000383          755 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.389, whole genome shotgun sequence.
ACCESSION   DADLSA010000383 DADLSA010000000
VERSION     DADLSA010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.389"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..491)
                     /locus_tag="JN691_004437"
     CDS             complement(<1..491)
                     /locus_tag="JN691_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY7245622.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKH"
     gene            complement(638..714)
                     /locus_tag="JN691_004438"
     tRNA            complement(638..714)
                     /locus_tag="JN691_004438"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(678..680),aa:Pro,seq:tgg)
BASE COUNT          186 a          192 c          188 g          189 t
ORIGIN      
        1 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
       61 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
      121 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
      181 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
      241 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
      301 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
      361 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
      421 ttcagattac aatcggaacc gctcggtttc gccatcacat gaaaagcacg cgttggaacc
      481 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
      541 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
      601 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
      661 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
      721 tgctttttga atttttagtt caattcttta aagtc
//
LOCUS       DADLSA010000384          752 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.390, whole genome shotgun sequence.
ACCESSION   DADLSA010000384 DADLSA010000000
VERSION     DADLSA010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.390"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..128
                     /locus_tag="JN691_004439"
     CDS             <1..128
                     /locus_tag="JN691_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HAY7245623.1"
                     /translation="LTGAAFSLVMPFLPLYVEQLGVTGHSALNMWSGIVFSIMGS"
     gene            152..>752
                     /locus_tag="JN691_004440"
     CDS             152..>752
                     /locus_tag="JN691_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7245624.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAF"
BASE COUNT          204 a          150 c          187 g          211 t
ORIGIN      
        1 ttcttaccgg ggccgccttc agtctggtaa tgcccttctt acccctctac gttgagcagc
       61 ttggtgttac cggccactcc gccctgaata tgtggtccgg tattgtcttc agtataatgg
      121 gttcctaaac acatcatttt aggtattgaa tatgagacgg gcaatcagct acatcagatt
      181 ttcaagtgaa cgacaactga aaggggactc tgtacgccga caatctaagc tggttactga
      241 ctggcttgat aagaaccctg agttttactt ggattcatcc ttgagcttta aagatttggg
      301 gaaaagtgct tttagtggta agcacctgaa aggtggtcta ggtgactttt taaccgctat
      361 cgagaaaggg cttgttaaag ctggtgatac gctactaatc gagagtttag accgtctgtc
      421 acgtcaggat attgatattg cttctgagct attacgccgt atcttaaggg ctggtgtaga
      481 tgtagtcaca ctgagcgatg gagaacatta cacacgagaa agtttaaaag acccgttagc
      541 cctgattaaa agcattctga taatgcagcg ggcgcatgag gaaagtttga ggaagtctga
      601 gcgtgtccaa gctgcatgga accgtaaaaa agaactcatc tccgaaggta tcaaggtatc
      661 taggcgttgt cctgcatggt taaggctgaa tgatgaccgt cgaacattca caatcattcc
      721 tgataaagtg gaagttgtta aacgagcatt tg
//
LOCUS       DADLSA010000385          749 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.391, whole genome shotgun sequence.
ACCESSION   DADLSA010000385 DADLSA010000000
VERSION     DADLSA010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.391"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..342
                     /locus_tag="JN691_004441"
     CDS             <1..342
                     /locus_tag="JN691_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7245625.1"
                     /translation="AKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRRR
                     GIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIAR
                     VRSVEEILHAL"
BASE COUNT          218 a          176 c          167 g          188 t
ORIGIN      
        1 gctaaagtgt tgcccgaaaa ctggtggcaa catccagctg cattaggtgc aactgacagt
       61 gatatcgaaa tcatcaaacg tcagtggggt gcgttttacg gtacggatct ggagttgcaa
      121 ctgcgccgcc gaggtatcga tacaatagtg ttatgtggga tctcgaccaa tatcggtgtt
      181 gaatccaccg cccgcaatgc ctgggaactt ggctttaatc tggtgattgc cgaagacgcc
      241 tgtagtgccg ccagcgccga gcaacacaat aacagcatta atcatatcta cccgcgcatc
      301 gcccgtgtgc gtagcgtgga agagatcctt cacgcgttat gatttacatc ggtctaccgc
      361 aatggtcgca tcctaaatgg gtgcggttgg ggatcaccag ccttgaagag tatgcccgcc
      421 actttaactg cgtgatacgg gatttttaaa aatcactaaa gaacgcccaa gagcatgtgt
      481 tttctttaga ataatcaaag cgttggattg ccatgtcata tgaaaatcgg caaagttcat
      541 atgtaacaaa gtgtcccaat catgccccaa aatgccccac aacaaatatt tttgccccat
      601 gcatgcccca aaaaatcact ttccctcact cttgacactg tttatccata cagttaaaaa
      661 taatactgta taaaaacaca gtgtagaggg gtttttatgc gtattgaaat ctgcatagcc
      721 aaagagaaaa tgactaaaat gccaaccgg
//
LOCUS       DADLSA010000386          748 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.392, whole genome shotgun sequence.
ACCESSION   DADLSA010000386 DADLSA010000000
VERSION     DADLSA010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.392"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..538)
                     /gene="rclC"
                     /locus_tag="JN691_004442"
     CDS             complement(<1..538)
                     /gene="rclC"
                     /locus_tag="JN691_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HAY7245626.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAR"
     gene            complement(550..741)
                     /locus_tag="JN691_004443"
     CDS             complement(550..741)
                     /locus_tag="JN691_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7245627.1"
                     /translation="MAFSTNADDKIILKHISVSSVSASPTVLEDAIADIARKYNASSW
                     KVTSMRIDNNSTATAVLYK"
BASE COUNT          215 a          176 c          155 g          202 t
ORIGIN      
        1 cccgcgccga atctgccatt atcatgacag cacctgccag catcagagta tctttcaata
       61 ccaaacgacc agcaccggat aaataaggga aaccatgatg agcgtcaccc aatgcgggta
      121 cccatgcctc cggggtggtg attaaaaatg agagtgttac cagcggcgtg gtaaatgcca
      181 tcagtcctcc caataaacct aaccagcgat tgacaggatt agccaaaacc agcagagcaa
      241 taatcacctc cacgacgcca agaccgttgg aaaaaccata ggtattattg gccgtttgcc
      301 atgcccttgc ttctggtttg tattcgcctt cgtgagtcag atactgttta tagtcttccg
      361 ggtgttcata aaagaacgac attagtggac tgtttgcgac gaatggtgta atgctgtctg
      421 cctcgtaagg gacaaacttt aataacccaa tccacataaa aacaattgca atactcagac
      481 gaatcaatgt caggccaatt ttatctcccc gacttaacag gtgcaggtat ttttccattg
      541 tgaacatcct tatttataca atactgctgt tgcggttgaa ttattatcaa ttcgcatcga
      601 tgtgactttc caggatgaag cattatattt tctggctata tcagcaatgg catcctccag
      661 aactgtcggt gatgctgata ctgacgagac gctgatatgt ttcagaatta ttttatcatc
      721 tgcattggtg gaaaatgcca taacgcca
//
LOCUS       DADLSA010000387          740 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.393, whole genome shotgun sequence.
ACCESSION   DADLSA010000387 DADLSA010000000
VERSION     DADLSA010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.393"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(175..>740)
                     /locus_tag="JN691_004444"
     CDS             complement(175..>740)
                     /locus_tag="JN691_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAY7245628.1"
                     /translation="IEKAHHDVHELFFQVFDKGAMEDGEGVHIDFKNTTLLLTTNAGA
                     ELISQLCEDPTLMPDSAGLKEALMPELRKHFPAAFLGRVTVIPYLPLDETARGVIARL
                     HLGRLMDRMAEQHGVSLVYGDELITHIVSRCPMHETGARLLIGYIEQHILPQLSRYWL
                     QAMTEKTAITQIRIRVDDDEQVIFETD"
BASE COUNT          194 a          202 c          172 g          172 t
ORIGIN      
        1 gggagggcga tatgttacag agatactgac ccttgcacgc gcattaccaa gcgcgcgcaa
       61 aaaattatct actgatgcac gaaaagtccc ctgcaaatca ttagtggaag aacttccagg
      121 aaagcgactg acccacagtg cgccacttaa ttcttttgcc ataaggatgc tccattaatc
      181 cgtttcaaaa ataacctgtt catcgtcatc aacacgaatg cggatttgcg tgatggccgt
      241 tttctctgtc atcgcctgca accagtaacg ggacagttgt gggaggatat gttgttcgat
      301 atagccgatg agcaaacgag cgccggtttc gtgcatcggg cagcgcgaca caatatgcgt
      361 aatcaattca tcgccataca ccagtgaaac gccatgctgc tccgccatcc gatccatcaa
      421 tctgccaaga tgcaggcggg caataacacc acgcgcggtc tcatccagcg gtaaataagg
      481 aataaccgtc acacgcccca ggaaagccgc cgggaagtgt ttacgtaact ccggcattaa
      541 cgcctctttt agtcctgccg aatcaggcat cagcgtcgga tcctcacaga gctggctgat
      601 aagttccgct cccgcgttag tagtcaacaa cagcgtggta tttttgaaat cgatgtggac
      661 accttcgcca tcttccatcg cccccttatc aaacacctgg aagaacagct cgtggacgtc
      721 gtgatgggct ttttcaattt
//
LOCUS       DADLSA010000388          729 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.394, whole genome shotgun sequence.
ACCESSION   DADLSA010000388 DADLSA010000000
VERSION     DADLSA010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.394"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(247..>729)
                     /gene="sseA"
                     /locus_tag="JN691_004445"
     CDS             complement(247..>729)
                     /gene="sseA"
                     /locus_tag="JN691_004445"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HAY7245629.1"
                     /translation="GLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASH
                     ENTPQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIF
                     FGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK
                     "
BASE COUNT          185 a          199 c          161 g          184 t
ORIGIN      
        1 ttcggactaa taagccaggt tattttatta ccgaacacgg cggaaagggc cggtgtcgat
       61 aatacgctaa ataaacaata attactctct tttgcttgac aaaaagagag ttactggtga
      121 gtattgtttt gctgtgaagc acctgcgttg ctcatgtaac acaagtcaac ctgctggaaa
      181 ggccgcgaac cagaccagca ataaaaaaac gccaaattcg gcggtttttt attgcctgta
      241 aaatacttat ttcactggct caaccggtaa atctgcccgc gcgccccatt cactccatgc
      301 gccgtcgtac agtttcacgt ttggcacatc cagcgtcgcg agtgctaaca aaaccacggc
      361 tgccgttaca ccagagccgc agctgacgat aatcggtttg tcatagctga cgccgcgacc
      421 aaaaaatatc gcatccagtt catcggtcgt ttttagttcg ccttcgcgca ccagttccgt
      481 ccacggaacg ttcaatgcac cgggaatatg tccgcgacgt aaacctgggc gaggttcatc
      541 aacttctgcg ttaaaacgtg cagccgggcg ggcatcaata atttgcggcg tattttcatg
      601 gcttgccaat aacacatcgg ttactttcac cactgcttca ggattaaacg cggcgttaaa
      661 ctctccttcc ggcagctcta ctgcaccttc ttctaatagc aggtcatcgc gctgccagcc
      721 tgcaagtcc
//
LOCUS       DADLSA010000389          724 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.395, whole genome shotgun sequence.
ACCESSION   DADLSA010000389 DADLSA010000000
VERSION     DADLSA010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.395"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..517)
                     /locus_tag="JN691_004446"
     CDS             complement(<1..517)
                     /locus_tag="JN691_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAY7245630.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSR"
BASE COUNT          202 a          169 c          176 g          177 t
ORIGIN      
        1 ccctcgagcc gctatgaacc agcagttgca gatgctgcgc atccagacct gccagtgcaa
       61 acagttcagc gttgatgatt tgatcaactt gttgcagttc agcgaaatga ttgccaccac
      121 cgatagagcc aagcgagctg cgccacggat gctgtgcaaa tgctgacggc aggttttcct
      181 ccagccagct ttcttcagca acgtcatcca aagctgataa tcgcttttca aacttatcgg
      241 cgttgtattt gcgagcgaga atatctgttt gccatagcgc catgccacag ccgatatcat
      301 tgccgaccag tgctgggtaa aaacggcctg cggagaagaa cgctgcgcca atcgggtagc
      361 cgcgtccggg gtgtaaatct ggcatcccca ctacgcgctg catgttgggt aaatttgccg
      421 tggtgtgtaa ttgttggatc gctaaactct cgatccacag gtcatcagat gcgatggtaa
      481 ataccgcatc ggataaggga cgaatataat tgcccatata ccattccgta aatagactcg
      541 tcatacttca agttgcatgt gctgcgactt gaattattga gagtaacaat taaaaaatca
      601 ggaaatggca gacgaatcca tggcaagaaa aaaagaatta atcctggaaa ggtctgcaaa
      661 gagtcattga taaaggattt gtcatgattt acctccttgc agtttgtgga ataagaaaac
      721 gcgc
//
LOCUS       DADLSA010000390          714 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.396, whole genome shotgun sequence.
ACCESSION   DADLSA010000390 DADLSA010000000
VERSION     DADLSA010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.396"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..>714
                     /locus_tag="JN691_004447"
     CDS             <1..>714
                     /locus_tag="JN691_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7245631.1"
                     /translation="AGACRFVFNRALALQNENHEAGNKYIPYGKMASWLVEWKNATET
                     QWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPRFKKRGQNAAFRYPQGVKLDQENSR
                     IFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWYISIQTESEVSTPVHPSASMIGLDA
                     GVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCI
                     ANIRRDYLHKVTTTVSKNH"
BASE COUNT          204 a          181 c          176 g          153 t
ORIGIN      
        1 tcgcaggcgc atgtcgtttc gttttcaatc gtgctctggc gcttcagaat gagaatcatg
       61 aggccggtaa taaatacatc ccttacggga aaatggcttc ttggctggtt gagtggaaaa
      121 acgccactga aacgcaatgg cttaaagatt cgccctcaca gccattgcaa cagtcactga
      181 aagaccttga gcgggcttac aaaaacttct tccagaagcg ggctgctttt ccccgattca
      241 aaaagcgggg acagaatgct gcattccgct acccgcaggg cgttaagctc gatcaggaaa
      301 acagccgtat ttttctgccg aaacttggct ggatgcgcta ccggaatagc cgtcaggtca
      361 cgggagttgt gaaaaatgtc actgtcagcc agtcctgcgg tacgtggtac atcagtattc
      421 agacagaaag tgaagtatca actccggttc acccttcagc atcaatgatc gggctggatg
      481 ctggcgtggc taaactcgcc acgctgtcag atggcacagt ctttgagcct gtaaacagtt
      541 ttcagaaaaa ccagaagacg ctggcgagac ttcagcgaca gttaagccgg aaggtcaaat
      601 tcagcaacaa ctggcagaag cagaaacgca aaatacagcg actgcattcc tgtatcgcaa
      661 atatccgcag ggactacctt cacaaagtca caacgaccgt cagcaaaaac cacg
//
LOCUS       DADLSA010000391          695 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.397, whole genome shotgun sequence.
ACCESSION   DADLSA010000391 DADLSA010000000
VERSION     DADLSA010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.397"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            43..>695
                     /locus_tag="JN691_004448"
     CDS             43..>695
                     /locus_tag="JN691_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HAY7245632.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFY"
BASE COUNT          169 a          184 c          185 g          157 t
ORIGIN      
        1 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
       61 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
      121 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg
      181 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
      241 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc
      301 cgctgggcgt atctgtaccg ggccgtcgac agccggggcc gcactgtcga tttttatctc
      361 tcctcccgtc gtaacagcaa agctgcatac cggtttctgg gtaaaatcct caacaacgtg
      421 aagaagtggc agatcccgcg attcatcaac acggataaag cgcccgccta tggtcgcgcg
      481 cttgctctgc tcaaacgcga aggccggtgc ccgtctgacg ttgaacaccg acagattaag
      541 taccggaaca acgtgattga atgcgatcat ggcaaactga aacggataat cggcgccacg
      601 ctgggattta aatccatgaa gacggcttac gccaccatca aaggtattga ggtgatgcgt
      661 gcactacgca aaggccaggc ctcagcattt tatta
//
LOCUS       DADLSA010000392          690 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.398, whole genome shotgun sequence.
ACCESSION   DADLSA010000392 DADLSA010000000
VERSION     DADLSA010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.398"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..471)
                     /locus_tag="JN691_004449"
     CDS             complement(<1..471)
                     /locus_tag="JN691_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245633.1"
                     /translation="MRLWADGRLIFEGDITDAYADYNQAPDIPLILTGQIHFNLRNQP
                     AADFSAKGDVAVADIIRALASTVGLGFENQGGSRSLSDPHFSGNVVQQMLDVASAADI
                     NIDLGNVEKVTIWPKGQNRNIPPVLISPDHGLTGYPVYTMTGLSATTIFCPDLFT"
BASE COUNT          168 a          195 c          165 g          162 t
ORIGIN      
        1 agtaaagaga tcggggcaga atatcgtggt ggcgctgagt ccggtcatgg tgtaaaccgg
       61 atagccagtc agtccgtgat ccggcgaaat cagtaccggc ggaatattcc ggttctgccc
      121 tttcggccag atggtgactt tctccacatt ccccagatca atgttaatat cggcggctga
      181 agcgacatcc agcatttgtt gtacaacatt accggaaaaa tgcgggtcag acagactgcg
      241 gctgcccccc tggttttcaa atccaagccc gacagttgac gccagcgcac ggataatatc
      301 tgcaaccgca acatcgccct ttgcgctgaa atctgccgcc ggctgattac gcagattaaa
      361 atgaatctgt cctgtcagaa tgagcggaat atccggcgcc tggttgtagt cggcgtatgc
      421 atcagtaata tcaccttcaa aaataagccg cccgtcggcc cacagccgca ttttatttgc
      481 ggtactctgc tcctgtcgcc acacaccgcg ccagcttaaa ttcgtcatat gcgccggtga
      541 cattccccag atatacagcg tgatttgtgt accagccacc cctccatata cggcaatact
      601 ggcaaaacag cttgcgtcgg taatggtcag catattgccc tggccattac caaacgttcg
      661 tccgtctgaa agagtgaact ccaccgttat
//
LOCUS       DADLSA010000393          675 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.399, whole genome shotgun sequence.
ACCESSION   DADLSA010000393 DADLSA010000000
VERSION     DADLSA010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.399"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..>675)
                     /gene="ospC4"
                     /locus_tag="JN691_004450"
     CDS             complement(<1..>675)
                     /gene="ospC4"
                     /locus_tag="JN691_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HAY7245634.1"
                     /translation="LNLSIEAPSGARSSFRSLEHLDKVSRHYISEIIQKVHPLSSDER
                     HLLSIIINSNFNFRHQSNSNLSNNILNIKSFDKIQSENIQTHKNTYSEDIKEISNHDF
                     VFFGVEISNHQEKLPLNKTHHTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHE
                     HQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFR
                     EFCYNK"
BASE COUNT          225 a          104 c          116 g          230 t
ORIGIN      
        1 tttttgttat aacaaaactc tctgaatcct tgatctttac tctttcttat aaaatcaata
       61 agatgcaacc caattccaag cctcatatct ttagtgttaa atattggaac atcagttttc
      121 ccctgattac catgcacata cctgctaact tcatccacaa cctctttaaa gttatctaag
      181 aaaaatgatt ggtgctcatg gtaaaaaaca ggtggaatag cattatcaaa gtgatcggtt
      241 aatgtcatat atccatatgg agagtcatga tcaatgatat acgcattggc gccaaaatct
      301 acagtatgat gtgttttatt tagtgggagt ttttcttgat gattggaaat ttcaacccca
      361 aaaaaaacaa aatcgtggtt agagatttct ttaatatcct cagagtatgt attcttatgt
      421 gtttgtatat tttcagactg aattttatca aaggatttaa tattaagaat attatttgac
      481 agattagagt tactttgatg tctgaagtta aaattagaat taataataat agaaagtaag
      541 tgacgttcgt ctgaagaaag aggatgaact ttttgtatta tctcagagat atagtgcctg
      601 gacaccttat cgagatgctc aagtgaccga aatgatgacc gtgctccaga tggcgcttca
      661 atagaaagat ttagt
//
LOCUS       DADLSA010000394          658 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.400, whole genome shotgun sequence.
ACCESSION   DADLSA010000394 DADLSA010000000
VERSION     DADLSA010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.400"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            1..566
                     /locus_tag="JN691_004451"
                     /pseudo
     CDS             1..566
                     /locus_tag="JN691_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          169 a          136 c          161 g          192 t
ORIGIN      
        1 gctggtttga gttacaaaga ttttggttca ttcgactaca gtcgtaatgt cggtgtcgcg
       61 tatgatgcag aagcatttac tgatatgttt gttgagtggg gcggtgactc atgggctggt
      121 actgacctgt tcatgaccaa ccgtaccaac ggtgttgcaa cctatcgtaa cacagatttc
      181 tttggcatgg ttgaaggtct aaattttgct ctgcaatatc agggtaaaaa tgaaggtaca
      241 ggcaactaca aagcaaatgg cgatggccat ggtttatctg caacggtttc tcatttgctg
      301 gtgcttatgc aaactctgac cgtactgatt ggcagtctgg tgatggtaaa ggcgaaagag
      361 ccgaagtttg ggcgttgtca acaaaatatg acgctaacaa tgtttatgct gctgtgatgt
      421 acggtgaatc tcacaacatg aattccgacg acggtgatgt tgtgaacaaa acacaaaact
      481 tcgaagctgt tctgcaatat cagttcgact ttggtctgcg tccatccatc ggttactcct
      541 attctaaagc actggacgta gcaggttgga tttgccccta tatttccaga cacctgttat
      601 aacttaaccc attactggct tgctgccgta gatattcccg tggcgagcga taacccag
//
LOCUS       DADLSA010000395          652 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.401, whole genome shotgun sequence.
ACCESSION   DADLSA010000395 DADLSA010000000
VERSION     DADLSA010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.401"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            219..>652
                     /locus_tag="JN691_004452"
     CDS             219..>652
                     /locus_tag="JN691_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245635.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGAL"
BASE COUNT          150 a          144 c          158 g          200 t
ORIGIN      
        1 cctaaaacaa caatatggac atcttaacga tactctgcgc ctgcgtgtgc ggaacagaaa
       61 cccttacccg atatacttgt tatgccagag gtagcaattg cgttagcggc gtactgttgc
      121 tgtaaagtat aacgcctata ttttacagtt cagtcctctt ttgcttatta caaccttaag
      181 tatatcaagc atataaagat aataagagac tgaacaatat ggttacctgg acccaaatgt
      241 atatgccgat gggaggactg gggctatccg ctctggtcgc cctgatcccg ataatattct
      301 tcttcgttgc actcgcggta ttacgtctga aaggacatgt cgctggagca ataaccctta
      361 tattatctat cctgattgcg atattcgcct ttaaaatgcc gattgatatg gcatttgctg
      421 ctgcgggcta tggctttatt tatggattat ggccaattgc gtggattatt gtcgcggcgg
      481 tgttcctgta taaattaacc gttgccagcg ggcagttcga tattatccgc agctcggtta
      541 tctccatcac cgacgatcag cgtttgcagg tgttactgat tggtttctcc tttggtgcgt
      601 tgctggaagg agcggctggc tttggtgcgc cggtggcgat taccggtgcg ct
//
LOCUS       DADLSA010000396          650 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.402, whole genome shotgun sequence.
ACCESSION   DADLSA010000396 DADLSA010000000
VERSION     DADLSA010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.402"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..>650
                     /locus_tag="JN691_004453"
     CDS             <1..>650
                     /locus_tag="JN691_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HAY7245636.1"
                     /translation="IATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHV
                     AIPNYLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSR
                     LTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSP
                     MERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENR"
BASE COUNT          162 a          160 c          199 g          129 t
ORIGIN      
        1 atcgccacaa tggcaacccg gagaggctac cagatgggac gctggcttgc tggcaggctc
       61 atgaaagagc tggggctggt cagctgtcag cagccgactc accggtataa acgtggtggt
      121 catgaacatg ttgctatccc taactacctt gaaaggcagt tcgccgtgac cgagccaaat
      181 caggtgtggt gcggtgatgt gacctatatc tggacgggta agcgctgggc gtacctcgcc
      241 gttgttctcg acctgttcgc aagaaaacca gtgggctggg ccatgtcgtt ctcgccggac
      301 agcaggctca ccatgaaagc gctggaaatg gcatgggaaa cccgtggtaa gcccggcggg
      361 gtgatgttcc acagcgatca gggcagtcat tatacgagca ggcagttccg gcagttattg
      421 tggcgatacc agatcagaca gagtatgagc cggcgcggaa actgctggga taacagccca
      481 atggaacgct tcttcaggag tctgaagaac gaatggatgc cggtggtggg ttacgtaagc
      541 ttcagcgagg cagctcacgc cataacggac tatatcgttg gatattacag cgcactaaga
      601 ccgcacgaat ataacggtgg gttaccccca aacgaatcgg aaaatcgata
//
LOCUS       DADLSA010000397          649 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.403, whole genome shotgun sequence.
ACCESSION   DADLSA010000397 DADLSA010000000
VERSION     DADLSA010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.403"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(54..242)
                     /locus_tag="JN691_004454"
     CDS             complement(54..242)
                     /locus_tag="JN691_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245637.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            358..649
                     /locus_tag="JN691_004455"
                     /pseudo
     CDS             358..649
                     /locus_tag="JN691_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          172 a          154 c          181 g          142 t
ORIGIN      
        1 gtgaacagac gtttcagcgg gcggggagta aaagcggaca acatgaggac ggattaaccc
       61 ccggtatcct gtagccgggt aagggctgct gtgtcgccac ggcgctccag gatggaggcc
      121 gcgaccatcg caggtggtgg gtccatgggt ttgagcatct tcatccagtc ccattcacgt
      181 tgtgagagct tctcagcacc aacagagagc agaacggtat ctatactttt gcgggtcaac
      241 ataagaggat gagtccttaa taaaacaggt cctcagagca tacatattct gcggagcgtg
      301 cggcaacaac aggacgaaca ggggcggttt tcaatttgca gccgtcaggc tgccgtggtt
      361 cagcgcctga tgggaatcct ctcacttaag gcagcgatta aggtcaagcg ataccgctct
      421 tacagaggag aggtagggca aaccgccccc aatgttctcc aaagagattt caaggctacg
      481 cggccaaacg agaagtgggt taccgatgtt actgaatttg cagtcaatgg gcgcaagctg
      541 tatttgcctc cagtaataga tctcttcaac aacgaagtta tttcttacag cctttcggaa
      601 agaccagtga tgaacatggt cgagaatatg ctcgatcagg cattcaaaa
//
LOCUS       DADLSA010000398          620 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.404, whole genome shotgun sequence.
ACCESSION   DADLSA010000398 DADLSA010000000
VERSION     DADLSA010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.404"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..213
                     /locus_tag="JN691_004456"
     CDS             <1..213
                     /locus_tag="JN691_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245638.1"
                     /translation="PQDVQHFLSNAEMCQHLAGEWDSSLPEKDKRDIEKGVDTWCPPA
                     KKSLPVLREKYKENKEIIKILSAYDF"
     gene            275..>620
                     /gene="tssB"
                     /locus_tag="JN691_004457"
     CDS             275..>620
                     /gene="tssB"
                     /locus_tag="JN691_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HAY7245639.1"
                     /translation="MAINNSAQKFIARNRAPRVQIEYDVEIYGSEKKIELPFVMAVLA
                     DLAGKPREELPPVTDRKFLDIDIDNFNERMKAIAPRVAFAVPNTLTGEGQLMVDITLE
                     NMDDFSPAQIARK"
BASE COUNT          168 a          142 c          170 g          140 t
ORIGIN      
        1 ccacaggatg tgcagcattt tctgagtaat gcggaaatgt gccagcatct tgctggtgaa
       61 tgggacagtt ctttaccaga gaaggataaa agagacatcg aaaagggggt tgatacctgg
      121 tgtcctccgg cgaagaaatc actccctgtg ctgcgggaaa agtacaaaga aaacaaagag
      181 attataaaaa tactctctgc gtatgatttt taaactgtac cgtcggacca ctccccaggt
      241 ggtcgtcggc tgactgataa ggaaatgaag gattatggct atcaacaaca gcgcgcagaa
      301 gtttattgcc cgcaaccgcg cgccccgcgt gcagattgag tatgacgttg agatttacgg
      361 ctccgagaaa aaaatcgaac tgccgtttgt catggcggtg ctggccgatc tggccggtaa
      421 accacgcgaa gagctgccac cggtcaccga tcgtaaattc cttgatattg atatcgataa
      481 cttcaacgag cggatgaaag ccattgcgcc gcgcgtggcg tttgctgtcc ccaatacgct
      541 gacgggcgaa gggcagttaa tggtcgatat cacgctggag aatatggacg atttttcgcc
      601 tgcgcagatt gcccgtaagg
//
LOCUS       DADLSA010000399          616 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.405, whole genome shotgun sequence.
ACCESSION   DADLSA010000399 DADLSA010000000
VERSION     DADLSA010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.405"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..489
                     /locus_tag="JN691_004458"
     CDS             <1..489
                     /locus_tag="JN691_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7245640.1"
                     /translation="EKMAAMFHQQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSV
                     RQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKH
                     FLTIEVRPPMDACIASADNKMIARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADP
                     YP"
BASE COUNT          181 a          124 c          138 g          173 t
ORIGIN      
        1 gaaaaaatgg ccgccatgtt tcatcagcaa cgaaatccag tgattgatta tgtctggaat
       61 tctgtacggc gtaaattcgg ggggcgctta cattcccggg aggatgggat aaaaccattt
      121 attcagtcag tacgccaggg atactggggg tattaccttc cagatcagga tcatggtcct
      181 gaatacagtg aatttgctga tttttttgcg acctataaag cgacattacc aattattgga
      241 cgtctgatga acatcagtca ggctatgatt ataccgcttt tcccggttta tgatgaaaaa
      301 aaacatttcc tgactattga agttcggcca ccaatggatg catgcattgc cagcgcggac
      361 aataaaatga ttgcccgaca aatgaacaaa acagtggaaa ttttggtggg gtcacatccg
      421 gaacagtata tctgggtttt aaaattgtta aaaacgcgca aatcaaacga agcggacccg
      481 tacccttgaa attataaagt acaggtattt ccactagttg tttcttacag gttaccaatc
      541 gaaacacatc ccccttccgt gtgttgcccg tttggcacgg aggactgtgt agatgcagtt
      601 cgttaaattt aattta
//
LOCUS       DADLSA010000400          611 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.406, whole genome shotgun sequence.
ACCESSION   DADLSA010000400 DADLSA010000000
VERSION     DADLSA010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.406"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            31..393
                     /locus_tag="JN691_004459"
     CDS             31..393
                     /locus_tag="JN691_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAY7245641.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LRREMGLEEMTGFSKTTF"
BASE COUNT          192 a          141 c          138 g          140 t
ORIGIN      
        1 tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa cgaaatcaaa
       61 cgtctcagcg atcgcctgga cgccatccgc caccagcagg ccgatctgtc gctggttgaa
      121 gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc agaaattgct
      181 cgcctgcgtg aggttcatag ccaaaaactg agtaaagaag cacagaagct gatgaagatg
      241 ccgttccagc gcgcaattac caaaaaagag caggctgata tgggcaagct gaagaaaagt
      301 gttcgcggac tggtggttgt gcacccaatg accgcgctga ggcgcgaaat gggcctggaa
      361 gagatgactg gattttctaa gactacgttt taagcccctc agacgactgg ttcgatcgta
      421 tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt ctactcgcct
      481 aattaccaaa caattcactc cctttacttc gatttaccgc tggttgatat tctgaaaatg
      541 catttttacc gaaatagcca tctataaaca ataaaaatat atagaagata tcatttgtta
      601 attaacaagc a
//
LOCUS       DADLSA010000401          587 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.407, whole genome shotgun sequence.
ACCESSION   DADLSA010000401 DADLSA010000000
VERSION     DADLSA010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.407"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..491)
                     /locus_tag="JN691_004460"
     CDS             complement(<1..491)
                     /locus_tag="JN691_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7245642.1"
                     /translation="MAQPLSNPTDVNSEINAQDFMLRQFLGRHAFITLGRVVAVEEGF
                     IEARPMVMGVAADGSPVEHEVIYNIPVWRLQGGGNAVIMPPHVGDIGFLAICDRDISA
                     VKATRQAAMPGSKRTHNYADAIWLGGVLNGDPVQFVVFDDNQIRIVSPWKVEISAPEG
                     VINA"
BASE COUNT          139 a          179 c          132 g          137 t
ORIGIN      
        1 gcattaatca caccctcagg cgcggatatc tccactttcc agggagatac aatccgtatc
       61 tggttgtcat caaataccac aaactggacg ggatcaccgt tgagcacgcc acccagccag
      121 atggcatcag cataattatg ggtacgcttt gatcccggca tcgcagcctg acgcgtggct
      181 tttaccgcac tgatatcccg atcgcagatg gcaagaaaac cgatatcccc cacatgtggc
      241 ggcataatca ccgcattacc gcccccctgt agtcgccata cgggaatgtt ataaatcacc
      301 tcatgttcaa ccggggaacc gtctgctgca acgcccatta ccatcggtcg ggcctcaata
      361 aatccctctt ccacggctac cacccgtccc agagtgataa acgcatgacg tccgagaaac
      421 tgccgcagca taaagtcctg tgcattgatt tcgctgttca cgtctgtcgg gttactgagt
      481 ggttgtgcca ttttcttcag ccctcatcat ggtacagttg gaactccacg gaccgcccac
      541 ggttcgcgaa gtaatggtgt gtatcacccc tgtgatcgta taatcgc
//
LOCUS       DADLSA010000402          575 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.408, whole genome shotgun sequence.
ACCESSION   DADLSA010000402 DADLSA010000000
VERSION     DADLSA010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.408"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            77..>575
                     /locus_tag="JN691_004461"
     CDS             77..>575
                     /locus_tag="JN691_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7245643.1"
                     /translation="MLYAARKRGLEIGIFCTIHTLRLHFEEHLPLVVAGRRLGVPKST
                     VCSMFVRFRKAGLSWPLPAGMSERELDARLYGSASTVPVVLTESTVMPEVPGVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTL"
BASE COUNT          127 a          165 c          152 g          131 t
ORIGIN      
        1 cgcagctatt tagaacggca cggaaccacg gcagcttgac ggctgcaatg acgtgtgtcg
       61 tctggcggtg gagaatctgc tgtatgcagc ccgaaaacgg gggctggaaa tcggcatctt
      121 ctgcaccatc cacactttac gccttcactt tgaagaacat ttacccctcg ttgtggccgg
      181 acgtcgcctg ggcgtaccaa aatcaacagt ttgtagtatg tttgtgcgct tccgcaaagc
      241 tggcctttca tggcctctgc ccgcaggtat gtcggagcgg gaacttgacg cccgtcttta
      301 cgggagtgcc tccacagtac ctgtcgtact tactgaaagt accgttatgc ccgaagtccc
      361 cggggtaaaa aagcgtcccc ggcgccccaa cttcccttat gagttcaaaa tcgccttagt
      421 ggagcagtca ctgcagcccg gagcctgtgt ggcgcagatt gcccgtgaaa atggaatcaa
      481 cgataacctg ctcttcaact ggcgccatca ataccggaaa ggtggcctgc tgccttccgg
      541 aaaaaatatg ccggcactgc ttcccgtgac gttaa
//
LOCUS       DADLSA010000403          567 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.409, whole genome shotgun sequence.
ACCESSION   DADLSA010000403 DADLSA010000000
VERSION     DADLSA010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.409"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..>567
                     /locus_tag="JN691_004462"
     CDS             <1..>567
                     /locus_tag="JN691_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7245644.1"
                     /translation="KPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQLDIINT
                     AFKVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIPLYRQS
                     EIYARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNG
                     KTKTGRLWVYVRDDRNAGSSLPAAVWFA"
BASE COUNT          145 a          144 c          156 g          122 t
ORIGIN      
        1 aaaaccgctg ccggcagaac ttccccggga gacacatcgc cttctgcctg ctgaaaccag
       61 ttgcccggcc tgtggaggtg ttctgaaaga aatgggggaa acaatctcag agcaactgga
      121 tatcattaat accgccttta aagttatcga aaccatacgt cccaaactgg cctgtagccg
      181 gtgtgatgtc atcgttcagg caccacttcc ccctaaaccg atcgaacgcg gttatgccag
      241 tgcagggtta cttgcacgga tcctggtcag caaatatatg gaacatatcc ctttatatcg
      301 ccagtcagaa atatacgcgc gacagggcgt ggagctgagc cgtaatacca tggtgcgctg
      361 ggtatcagaa atggcagaca aactccgtcc tctgtatata gcgctgaatg actatgttct
      421 ggaggcagga aaggtgcacg cagatgacac tccggtgaaa gtactggccc cggggaacgg
      481 aaagacgaaa acgggtcgtc tgtgggtata cgtcagggat gatcgtaatg cgggttcatc
      541 cctgccggca gccgtctggt tcgcgta
//
LOCUS       DADLSA010000404          554 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.410, whole genome shotgun sequence.
ACCESSION   DADLSA010000404 DADLSA010000000
VERSION     DADLSA010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.410"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..>554
                     /locus_tag="JN691_004463"
     CDS             <1..>554
                     /locus_tag="JN691_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7245645.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEM"
BASE COUNT          125 a          134 c          162 g          133 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttatattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgtt
//
LOCUS       DADLSA010000405          549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.411, whole genome shotgun sequence.
ACCESSION   DADLSA010000405 DADLSA010000000
VERSION     DADLSA010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.411"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(1..365)
                     /locus_tag="JN691_004464"
                     /pseudo
     CDS             complement(1..365)
                     /locus_tag="JN691_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            288..539
                     /locus_tag="JN691_004465"
     CDS             288..539
                     /locus_tag="JN691_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245646.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
BASE COUNT          150 a          124 c          139 g          136 t
ORIGIN      
        1 gccagtgttg ttgcgccttg ctgccaacga agcgccactg ctcatcaagt tcacagataa
       61 gggcaacatc agcgtgagcg actggcgcag atgttatctg ccggggcgag agctttttaa
      121 tgtccgtatg acggtattga tgccgatttt cagtgttctg gcggtataac agccccctgc
      181 gccgttatgt gccatttcaa cgatctgctc cttaacgccg agtttctggc ttcataagag
      241 taagtgagct ggaaaacacg tttacatgag cgacaacgga aacgctcatg ccgggaacaa
      301 ctacggccat gtcgataaac ttcatcggaa ttacagcgcg gacaatgaac agtaacggta
      361 gtcatggaaa acctcaaata ccagtagatt accagaaact tctatcgatt gaagacatta
      421 ccttttgccg caataggtgg ggtaacattg gtgaaaacgc attacgccgg gtggcagttg
      481 gaaaaaaatt atcttttttc ggctctgatc gtggtggaga aaatgcggca ataatctaaa
      541 tagctgcgc
//
LOCUS       DADLSA010000406          549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.412, whole genome shotgun sequence.
ACCESSION   DADLSA010000406 DADLSA010000000
VERSION     DADLSA010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.412"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..207
                     /locus_tag="JN691_004466"
     CDS             <1..207
                     /locus_tag="JN691_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7245647.1"
                     /translation="VYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIVEGANHM
                     DLYDGKAYVAEAISVLAPFFEETL"
     gene            207..>549
                     /locus_tag="JN691_004467"
     CDS             207..>549
                     /locus_tag="JN691_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7245648.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGV"
BASE COUNT          144 a          113 c          148 g          144 t
ORIGIN      
        1 gtgtacctga ctcagccgat gcagattgtg gcgggcagcc aggcagggag caaatggatg
       61 agtgacgacc tgtacgatcg ggcatcaagc caggataaac gctatcacat tgttgaaggc
      121 gcaaaccata tggatcttta tgacggcaaa gcctatgtgg ctgaagcaat ttcagtatta
      181 gctccgttct ttgaggaaac tctgtaatga aacatattca aatccgcaat agtgatatgg
      241 cctggcatat tgctgccaat attcagtttc caccaaattt tgatgagtca aaacagtatc
      301 cggcgattat cagcgtccat ccttttggca gttgtaagga gcagacgtct ggcaatatct
      361 atggcaaggc attggctgaa aaaggctatg tcgttctcgc ttatgacgcc agtttccagg
      421 gagaatctgg tggggagcct cgttggatag aagatccgac acagcgcgta gaggatatta
      481 gccgtgttat cgattatgca gtaactctgc catatgttga tgctgaacgc attggcgtgc
      541 tgggtgtgt
//
LOCUS       DADLSA010000407          549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.413, whole genome shotgun sequence.
ACCESSION   DADLSA010000407 DADLSA010000000
VERSION     DADLSA010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.413"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..463
                     /locus_tag="JN691_004468"
     CDS             <1..463
                     /locus_tag="JN691_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7245649.1"
                     /translation="LGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLIDH
                     QAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYDQ
                     VERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT          142 a          106 c          120 g          181 t
ORIGIN      
        1 tctgggtggg gagatggata aagatatgat cgccattcga atcgggccag atataccaat
       61 ggctattgtt ggctcaccgg attatttttc tcgtcgaagt gctccaacgt cagtgtcaca
      121 attaatagat catcaggcaa ttaatttgta tcttcccaca tcgggtacag caaatcgctg
      181 gagattaata cgcggtggac gtgaagttcg tgttcgcatg gaaggtcagc ttttactgaa
      241 tacgatagac ctgatcattg atgctgcaat tgatgggcat ggattggcgt atctacctta
      301 tgatcaggtt gagcgggcta ttaaagaaaa aaaactgata cgtgttttgg ataaattcac
      361 tccagattta cccggttatc acctgtacta tccacaccgt cgacatgctg gctcggcatt
      421 ctcattattt atagataggc tgaagtataa aggtgctgtt tagcactact tgctgataca
      481 ttaatttaat tcttctctta acgtattctc agttcctttc aacgttttgg ttgtttttta
      541 ttcttcgta
//
LOCUS       DADLSA010000408          548 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.414, whole genome shotgun sequence.
ACCESSION   DADLSA010000408 DADLSA010000000
VERSION     DADLSA010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.414"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..>548
                     /locus_tag="JN691_004469"
     CDS             <1..>548
                     /locus_tag="JN691_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7245650.1"
                     /translation="QFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRH
                     QQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGR
                     TIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSG
                     PHYKVDSIRESWTSILKRAGLR"
BASE COUNT          178 a          117 c          120 g          133 t
ORIGIN      
        1 tcaatttgca acagataatg gttatacatc aggaaatcca tttaacggtc tgacaccatt
       61 aaaaaagtcc aagatagaac cagatcccct cacccgtgac gaatttattc gttttattga
      121 ggcttgccgt catcaacaaa caaaaaacct gtggattatc gctgtataca cgggtattcg
      181 tcacggggag ctggtatcac tggcctggga agatatagac cttaaagcga ggactataac
      241 catccgccgg aattatacaa aactaggcga attcactcca ccaaaaactg atgctggtac
      301 cggaagaacg attcatctag ttcaaccagc tattgatgct cttaaaagcc aagcggaaat
      361 gaccatgctt ggaaaacagc attctgtaga ggtaaagcag agggaatatg ggagaagtac
      421 tgtgcataaa tgcacttttg tttttagtcc tcaggtaata aaacagcggc agttttccgg
      481 accgcactat aaggttgact ccatcaggga gtcatggaca agtatcttaa aacgcgcagg
      541 tctgagac
//
LOCUS       DADLSA010000409          542 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.415, whole genome shotgun sequence.
ACCESSION   DADLSA010000409 DADLSA010000000
VERSION     DADLSA010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.415"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..466
                     /locus_tag="JN691_004470"
     CDS             <1..466
                     /locus_tag="JN691_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7245651.1"
                     /translation="VLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQ
                     RQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQKEAKLFFQVIAKRYEKSAMILTSNLPF
                     GQWDQTFAGDAALTSAMLGRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          152 a          131 c          137 g          122 t
ORIGIN      
        1 cgtattactg gggccatcag gtgtggggaa aacccatctg gcaatagcga tgggctatga
       61 agcagtccgt gcaggtatca aagttcgctt cacaacagca gcagatctgt tacttcagtt
      121 atctacggca caacgtcagg gccgttataa aacgacgctt cagcgtggag taatggcccc
      181 ccgcctgctc atcattgatg aaataggcta tctgccgttc agtcagaaag aagcaaaact
      241 gttcttccag gtcatcgcta aacgttacga aaagagcgca atgatcctga catccaatct
      301 accgttcggg cagtgggatc aaacgttcgc cggtgatgca gcactgacct cagcgatgct
      361 gggccgtatc ttacaccact cacatgtcgt tcaaatcaaa ggagaaagct atcgactcag
      421 acagaaacga aaggccgggg ttatagcaga agctaatcct gagtaaaacg gtggatcaat
      481 attgggccgt tggtggagat ataagtggat cacttttcat ccgtcgttga cacaacactg
      541 gc
//
LOCUS       DADLSA010000410          542 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.416, whole genome shotgun sequence.
ACCESSION   DADLSA010000410 DADLSA010000000
VERSION     DADLSA010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.416"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..91
                     /gene="yjdI"
                     /locus_tag="JN691_004471"
     CDS             <1..91
                     /gene="yjdI"
                     /locus_tag="JN691_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY7245652.1"
                     /translation="WVMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            103..375
                     /locus_tag="JN691_004472"
     CDS             103..375
                     /locus_tag="JN691_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7245653.1"
                     /translation="MEICEGHNNFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
BASE COUNT          173 a           95 c          146 g          128 t
ORIGIN      
        1 gtgggtcatg ccggatgaag tcgacgtcgc cactgtggtt aaagtgattg atacgtgccc
       61 gagcggcgcg ctgaaatacc gtcataaata agcgagggta aaatggaaat atgcgaaggg
      121 cacaataatt tttacattaa tgacgaacaa ggcaagcaaa tcgctgaaat tgtctttgtg
      181 ccgaccggag agaatttagc gattatcgaa cataccgatg tcgatgaaag cctgaaaggg
      241 caagggattg gtaaacagct ggttgcgaaa gtcgtggaaa aaatgcgtcg ggaaaaacga
      301 aaaattatcc cattatgtcc atttgcgaaa cacgaatttg ataaaacgcg ggagtatgat
      361 gatattcgca gttgatggga gagtacagag tcacgatatt tttcttgctc tccgcggtgt
      421 gatgcaggag agcatctgaa aggtaggggg atgcacaaag aatgggcaga gagcgcgttt
      481 ttttgtccca agtcatcccc tttactgagc aaaaaaaaga atatctccta tatgagaatc
      541 at
//
LOCUS       DADLSA010000411          541 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.417, whole genome shotgun sequence.
ACCESSION   DADLSA010000411 DADLSA010000000
VERSION     DADLSA010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.417"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(253..>541)
                     /locus_tag="JN691_004473"
     CDS             complement(253..>541)
                     /locus_tag="JN691_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7245654.1"
                     /translation="IGRNDPRKPISKESINQVIELLGYKGRLTGHGFRHTMSTILHEQ
                     GFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSRES"
BASE COUNT          169 a           94 c          100 g          178 t
ORIGIN      
        1 ttataatgat gtcagatgat tgaaatgcta aattatatct gaatcaactt ttcctttgct
       61 aaaggtatcg aaataactgc aaggctgtat tttgtaattg ccaatttaaa aatgtcaatt
      121 tgtttttaat tatgtttttg taataagttt gaagacgcaa aaaaataata taataacaaa
      181 aatcctccag ccttgtaggc cagaggataa aatacttaat tgaggaaaac atcagggaaa
      241 agtatttctg acttaacttt cccggctgga gagtgaatca atgtaatcag cgtaccattg
      301 catcatttca cggcggccat cgagatacag ggcatgatta taggtacccc tgatggaatt
      361 tttatccaca tgagctaact gcatttcaat ccaggcggaa ttaaagcctt gttcatgcag
      421 aattgtactc atggtgtgtc tgaaaccgtg ccctgtgagt cttcccttat atccaagtaa
      481 ttcaattact tggttaatgc tttccttact aattggtttg cggggatcat ttctaccgat
      541 a
//
LOCUS       DADLSA010000412          525 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.418, whole genome shotgun sequence.
ACCESSION   DADLSA010000412 DADLSA010000000
VERSION     DADLSA010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.418"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..373
                     /gene="ospC4"
                     /locus_tag="JN691_004474"
     CDS             <1..373
                     /gene="ospC4"
                     /locus_tag="JN691_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HAY7245655.1"
                     /translation="NKVTDKKMAHQALAYSLGNKKADIALYLLSKFNFTKQDVAEMEK
                     MNNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSK
                     DSKMIDFLLKNGAILGKRFEI"
BASE COUNT          194 a           77 c          100 g          154 t
ORIGIN      
        1 caataaagtg actgataaaa aaatggctca tcaggccctt gcttattctt taggcaataa
       61 aaaagctgat atagcacttt atttactatc taaatttaat tttacaaaac aagatgtcgc
      121 agagatggag aaaatgaaca acaatagata ttgtaatctg tatgatgttg aatacttact
      181 tagtaaagat ggtgccaact ataaagtgct agaatatttt atcaataatg gattggttga
      241 tgtaaacaaa aaatttcaaa aagcaaatag tggggacact atgcttgaca atgcaatgaa
      301 aagcaaagat tcaaaaatga ttgatttttt attaaaaaat ggagcaatat taggcaaacg
      361 atttgagata tgacctaaaa gctcggccaa ataataatgt cgtcgattat cgaagtcatc
      421 atgagtgatt ctgtccccac agtgcagcat cagcatgaca gtgaattgtc tggcgtggtc
      481 catttaatga gatggagatg gtccctcttt cctaaatagc tgtgc
//
LOCUS       DADLSA010000413          524 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.419, whole genome shotgun sequence.
ACCESSION   DADLSA010000413 DADLSA010000000
VERSION     DADLSA010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.419"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..453
                     /locus_tag="JN691_004475"
     CDS             <1..453
                     /locus_tag="JN691_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7245656.1"
                     /translation="IQAGTARKFTLSSANNSTKNAAFYLWGNPSRPVVAELGDDSGWH
                     FFSQRNTDNSITFAVNGQVIPLNYGNFDARYKHRTEGVQDVRYGYEMYYSPGSNTVSW
                     RFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
BASE COUNT          156 a          112 c          130 g          126 t
ORIGIN      
        1 attcaggccg gaactgccag aaaattcacg ttatccagcg ccaacaactc cacgaaaaat
       61 gcagcgtttt atttgtgggg taatccatcc aggcctgttg ttgcagagct tggtgatgat
      121 tcaggctggc attttttcag ccagaggaat acagataaca gcatcacgtt cgccgtaaac
      181 ggacaggtaa ttccgttaaa ttacggaaac ttcgatgccc gctataaaca tcgaactgag
      241 ggggtacagg atgtgcggta tggttatgaa atgtattaca gccccggcag taacaccgtt
      301 tcgtggagat tttgcgcacc ttcgggacac gggctgtcag ggatggcgat atcggatacc
      361 ggccgtaact cagcggataa cgttgacggt gtgtattacc gaccactgca aaaactgatt
      421 aatggcacct ggtataacgt agcgagtatt taacaatgtt gcatttaaaa aatattactg
      481 cgggtaatcc gaaaacagca gaacaatatc agatgacaaa acga
//
LOCUS       DADLSA010000414          517 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.420, whole genome shotgun sequence.
ACCESSION   DADLSA010000414 DADLSA010000000
VERSION     DADLSA010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.420"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            161..>517
                     /locus_tag="JN691_004476"
     CDS             161..>517
                     /locus_tag="JN691_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7245657.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLE"
BASE COUNT          159 a           94 c          112 g          152 t
ORIGIN      
        1 cgaaaacgat acgctttgct ggattatatc catgaaacta ctatccgctc tttggatgca
       61 tatcctgtac actcagaaca tcaggattta aattaaggtt tgatgtattc tctgaataaa
      121 acagtaatga taattttggt agaggcattc gcactaaata atgaaaaaat ataaatctga
      181 gtttattcct gaatttaaga aaaattacct ttcccctgtt tactggtcta catggttcct
      241 tttgggaatg attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc
      301 aaaaataggg cgttgggcgg gcagattgag caaaaaagct cgtcgcaggg cgacgattaa
      361 tttatcgctt tgtttcccgg aaaagagtga tacagaacgg gaaataattg tcgacaaaat
      421 gtttgccaca gcattgcaat ccatagtgat gatggcagaa ctggcaattc gtggtccgga
      481 aaagttccag aaacgagtgt tctggaaagg cctcgaa
//
LOCUS       DADLSA010000415          512 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.421, whole genome shotgun sequence.
ACCESSION   DADLSA010000415 DADLSA010000000
VERSION     DADLSA010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.421"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(39..176)
                     /locus_tag="JN691_004477"
                     /pseudo
     CDS             complement(39..176)
                     /locus_tag="JN691_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            237..430
                     /locus_tag="JN691_004478"
                     /pseudo
     CDS             237..430
                     /locus_tag="JN691_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          139 a          130 c          111 g          132 t
ORIGIN      
        1 atgtttccgt aaagtccctg caatttctga aataatcaac gtacagccag ggcttcgttg
       61 ttaatcgaac cataataacg aacttcgcca agccgttcag gtgcagcaat agcaactgcc
      121 aacgtttctt tatgtacatc cagcctgatg taagctgcaa tattttccga atgcataatg
      181 acctcctctg gaatatctca ccaatgctat agtgagtata taaaggtcat accatctaca
      241 tgttcttcag caacgatcac ggtggagagc gtggtgcgtt actgtacggt ctgatcggca
      301 cctgccgttt gaacggtatc gatccggaag cgtatctgcg ccatatcctg agtgcactgc
      361 ctgccggata tggccttcca accgtgttga cgaactgctg ccatggaacg tagttctcac
      421 caataaataa gcgtcaatac tgtgctccgt taacgcttac cactggccta tacggagcga
      481 ctaaaagaag caaaactgct ggcatcaaca gg
//
LOCUS       DADLSA010000416          510 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.422, whole genome shotgun sequence.
ACCESSION   DADLSA010000416 DADLSA010000000
VERSION     DADLSA010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.422"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..489
                     /locus_tag="JN691_004479"
     CDS             <1..489
                     /locus_tag="JN691_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7245658.1"
                     /translation="KKMAAMFHQQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSV
                     RQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKH
                     LLTIEIRPPMDACIASADNKMIARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADP
                     YP"
BASE COUNT          162 a          104 c          112 g          132 t
ORIGIN      
        1 aaaaaaatgg ccgccatgtt tcatcagcaa cgaaatccag tgattgatta tgtctggaat
       61 tcagtacggc gtaaattcgg ggggcgctta catgcccggg aggatgggat aaaaccattt
      121 attcagtcag tacgccaggg atactgggga tattacctcc cagatcagga tcatggtcct
      181 gaatacagtg aatttgctga tttttttgcg acctacaaag cgacattacc aatcattgga
      241 cgtctgatga acatcagtca ggctatgatt ataccacttt tcccggttta tgatgaaaaa
      301 aaacatctcc tgactattga aattcggcca ccaatggatg cttgcattgc cagcgcggac
      361 aataaaatga ttgcccgaca aatgaacaaa acagtggaaa ttttggtggg gccacatccg
      421 gaacagtatg tctgggtttt aaaattgtta aaaacacgca aatcaaacga agcggacccg
      481 tacccttgaa attataaagt acaagtattt
//
LOCUS       DADLSA010000417          483 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.423, whole genome shotgun sequence.
ACCESSION   DADLSA010000417 DADLSA010000000
VERSION     DADLSA010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.423"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(69..>483)
                     /locus_tag="JN691_004480"
     CDS             complement(69..>483)
                     /locus_tag="JN691_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7245659.1"
                     /translation="FDCVARPWLTEAGSRSARGIWEIEFNHKLLRYIYGLTNQFTTYS
                     LRDCGSLRNPRTIRLYESLAQFKSSGLWVTTHAWLNDRFLLPESQQKNLAELKRSFLD
                     PALKQINEKTPLLAKYSIDDSGKFLFSIIDKQNPV"
BASE COUNT          136 a           97 c          117 g          133 t
ORIGIN      
        1 gtgtggaaat tcgcccatct tcatgacaaa aatttgtcaa atgacaggct catgtgtgct
       61 gatttatgtc agacgggatt ttgcttatca attattgaga acagaaattt tcctgaatca
      121 tcaatactat acttagcaag taaaggtgtt ttctcattta tctgcttgag tgcaggatca
      181 aggaaagatc gtttcaactc tgccaagttc ttctgttggg attccggcaa aaggaaacgg
      241 tcatttaacc aagcatgagt agtaacccat aagcctgaag atttgaattg agcaagactt
      301 tcataaaggc ggatcgtccg tggatttcga agactgccac aatcgcggag cgagtaggtg
      361 gtgaactggt tcgtcaggcc gtaaatgtac cgcaggagtt tatggttaaa ttcgatttcc
      421 cagataccac gagctgatcg gctgccagcc tctgttagcc aggggcgcgc gacgcagtca
      481 aaa
//
LOCUS       DADLSA010000418          469 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.424, whole genome shotgun sequence.
ACCESSION   DADLSA010000418 DADLSA010000000
VERSION     DADLSA010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.424"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            82..>469
                     /gene="yiaM"
                     /locus_tag="JN691_004481"
     CDS             82..>469
                     /gene="yiaM"
                     /locus_tag="JN691_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HAY7245660.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTS"
BASE COUNT          118 a          103 c           95 g          153 t
ORIGIN      
        1 ttattgctct aactatatta ataatttagg ttgctgcaca tgcaacttga attatgacag
       61 acatagcctc aaggaatagc tatgaaaaaa atacttgaag caatactggc gattaatctc
      121 gccgtacttt cctgtattgt gtttataaac attattttaa gatatggttt tcagacaagc
      181 attttatctg tagatgaatt gtcacgttat ttatttgtct ggttaacatt tattggcgcg
      241 attgtggctt ttatggataa cgcccacgtt caggtcacct ttctggtgga gaaactctcc
      301 cccgcatggc aacgacgagt cgctctggtt acccattcct taatcttatt tatttgtggc
      361 gcactggcct ggggcgcaac gctaaaaaca attcaggact ggagcgatta ttcaccgatc
      421 ctcggtttgc ccatcggcct gatgtatgcc gcttgtctgc ccaccagcc
//
LOCUS       DADLSA010000419          460 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.425, whole genome shotgun sequence.
ACCESSION   DADLSA010000419 DADLSA010000000
VERSION     DADLSA010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.425"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(15..251)
                     /locus_tag="JN691_004482"
     CDS             complement(15..251)
                     /locus_tag="JN691_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HAY7245661.1"
                     /translation="MLSQYAASPRLNALINSFNAALSPDSFISDFYGLIWNIDTAEKY
                     GLDVWGKIVGVSRRLTVKDDFNYLASASPGWTPR"
     gene            complement(274..>460)
                     /locus_tag="JN691_004483"
     CDS             complement(274..>460)
                     /locus_tag="JN691_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245662.1"
                     /translation="ASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTVVTFGID
                     EIPVLDVSDISVKLQEA"
BASE COUNT          128 a          123 c          109 g          100 t
ORIGIN      
        1 acagggatca tccattaccg gggtgtccat cctggactcg ctgaagccag gtaattaaaa
       61 tcgtccttta ccgtcagccg gcgactgaca cccacaatct ttccccagac atcaagacca
      121 tacttttctg cggtatcgat gttccagata agaccataaa aatcactgat aaaactgtcg
      181 ggggaaagcg ctgcgttaaa gctgttaatg agggcattga gtcgggggct ggcagcatac
      241 tgcgaaagca cagtggcagc cacattctgc acgttatgcc tcctggagtt tcacactgat
      301 atccgacaca tccagaaccg gaatctcatc tatcccgaaa gtgacaaccg ttgtccatga
      361 cgaaccgtca cggctcacag taaggcccag aatatcaata tgttccggat cggttttgta
      421 aatgccggca tagtagcgcc cggcagagac aacagaggca
//
LOCUS       DADLSA010000420          455 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.426, whole genome shotgun sequence.
ACCESSION   DADLSA010000420 DADLSA010000000
VERSION     DADLSA010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.426"
                     /strain="HUE14"
                     /db_xref="taxon:623"
BASE COUNT          136 a           98 c           68 g          153 t
ORIGIN      
        1 ttctgtaatc ctctttgctt cctgagtaat aacttcctga gtgaatattt aacctgagct
       61 tgatcctaca catacttatt atgaatgata aaattcattc aattaataac acatatatta
      121 attgccgtta aaactaaaaa cagcatcaat aatcaacgcg atataataaa cctgccttac
      181 atatcaactg cgccagaggt aggattgaaa acgctctcct gattttccaa ttcattttct
      241 ggataaataa ataatttatt tttgtcacta ttatttatgt aatcatcctg tcagggagag
      301 ggatctcaat tatcaatgct taattacgtc atcattttga tgacatgaaa ccggagcatt
      361 ttgcatggaa ttttcgtttt cacccaaacg tcttgttgtt gctgtcgccg ccgctcttcc
      421 tctcatggcc agcgcagcag ataccccgtc aactg
//
LOCUS       DADLSA010000421          454 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.427, whole genome shotgun sequence.
ACCESSION   DADLSA010000421 DADLSA010000000
VERSION     DADLSA010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.427"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            161..>454
                     /locus_tag="JN691_004484"
     CDS             161..>454
                     /locus_tag="JN691_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7245663.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMM"
BASE COUNT          135 a           78 c           97 g          144 t
ORIGIN      
        1 cgaaaacgat acgctttgtt ggattatatc catgaaactg ctatccgctc tttggatgca
       61 tatcctgtaa actcagaaca tcaggactta aattaaggtt tgatgtattc tctgaataaa
      121 atattaatga tgattttggt aggggcattc gcactaaata atgaaaaaat acaaatccga
      181 gtttattcct gaatttaaga aaaattatct ttcccctgtt tactggttta catggttcgt
      241 tttgggaatg attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc
      301 caaaataggg cgttgggtgg gcagattgag cagaaaagct cgtcgcaggg cgacgattaa
      361 tttatcgctt tgtttcccgg aaaagagtga tacagaacgg gaaataattg tcgacaatat
      421 gtttgccaca gcattgcaat ccatagtgat gatg
//
LOCUS       DADLSA010000422          443 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.428, whole genome shotgun sequence.
ACCESSION   DADLSA010000422 DADLSA010000000
VERSION     DADLSA010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.428"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            157..>443
                     /locus_tag="JN691_004485"
     CDS             157..>443
                     /locus_tag="JN691_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245664.1"
                     /translation="MQSRSLLLDTGTWDILLDDTGNLAITDNPHAVAQDVACACSTFL
                     GECWYDSTAGIPYWPRILGHWPGTQLVNATLQQEALKLPTVSAAVCQVTAD"
BASE COUNT           97 a          124 c          128 g           94 t
ORIGIN      
        1 ggcgataccg ccgtcagtaa ggggcttgac gttaccgggc agtccaggct ttccggcggt
       61 tcgaacatcg ggggtattga ttttggaaac cacgttcacg gcggcgttga gtccggcggc
      121 tcaaccacgc agggaccccg gtaaacagga gaaaacatgc agtcacgatc acttcttctt
      181 gacaccggga catgggatat cctgcttgat gataccggaa atcttgccat tactgataat
      241 ccccatgcgg tagctcagga tgtggcgtgt gcgtgcagta cttttctggg ggagtgctgg
      301 tacgactcaa cggctggcat accttactgg ccacgcatcc tcggacactg gcccggcaca
      361 caactggtga atgccactct gcaacaggaa gcacttaaac tgccgaccgt gagcgccgct
      421 gtctgccagg tcacagctga tca
//
LOCUS       DADLSA010000423          442 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.429, whole genome shotgun sequence.
ACCESSION   DADLSA010000423 DADLSA010000000
VERSION     DADLSA010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.429"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            28..>442
                     /locus_tag="JN691_004486"
     CDS             28..>442
                     /locus_tag="JN691_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245665.1"
                     /translation="MSENKSFSTAVPAVQISDSGLNVPDEADILSGRLADFSAALGGA
                     MSTSLSSPQGQLASSESAIIADKNDQLLYIVNQINPDFSSGRFQDAIGRIYFLERRGA
                     TGTTVTATCTGLVGTLIPAGSMVQDEAGYKYVSQSD"
BASE COUNT          103 a          101 c          131 g          107 t
ORIGIN      
        1 caataatgac atttttacgg tactgctatg agtgaaaata aatctttttc taccgcagtg
       61 cccgctgtac agatttcgga tagcgggctg aatgtgccgg atgaagcgga tattctgagc
      121 ggcaggcttg ctgatttttc ggcggcgctg ggcggggcca tgagtaccag tctgagcagt
      181 ccgcagggac aacttgcatc cagcgaaagc gccattattg cggataaaaa cgatcagttg
      241 ctgtatatcg ttaaccagat caaccctgac ttttccagtg gtcgctttca ggatgcaata
      301 ggcaggattt atttcctgga gcgccgcggg gccacaggta cgacagtaac agcgacctgt
      361 accgggctgg ttggcacgct gatcccggcg ggcagtatgg tgcaggatga ggccggttat
      421 aagtatgtca gtcagtcaga cg
//
LOCUS       DADLSA010000424          436 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.430, whole genome shotgun sequence.
ACCESSION   DADLSA010000424 DADLSA010000000
VERSION     DADLSA010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.430"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(322..>436)
                     /locus_tag="JN691_004487"
     CDS             complement(322..>436)
                     /locus_tag="JN691_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7245666.1"
                     /translation="YLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          155 a           66 c           71 g          144 t
ORIGIN      
        1 gatgtgacaa catctatttt cattgatttg attcaatata acaatagatt acagaggatt
       61 aacttccagt tacttaaaaa ataaagaaaa aagtgtggaa acaggagaat ttaagtgatg
      121 tgtaaactta tgaatgattt tgtttattta ttttgaagaa cataaaccat tagattttgt
      181 atgggtatgg ttaacttaat gtttattatg ataattaaga atcacactta caataacatc
      241 aattaataaa tggttaatca tttttgatat atcgtaagaa taatgtagtt tttaacacca
      301 tccctggtat ctcaactatc tctataaaac agcatgacgc tgtcgtcctc tggctctatc
      361 ccagatgccg taaaaacgcc ctgcattgct ggcggtatac cagacagtat gacgaatatt
      421 gcgatgccca agataa
//
LOCUS       DADLSA010000425          429 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.431, whole genome shotgun sequence.
ACCESSION   DADLSA010000425 DADLSA010000000
VERSION     DADLSA010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.431"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..>429
                     /gene="ipaH9.8"
                     /locus_tag="JN691_004488"
     CDS             <1..>429
                     /gene="ipaH9.8"
                     /locus_tag="JN691_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY7245667.1"
                     /translation="LDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFAVAADA
                     TESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLEILEDIARDKVR
                     TLHFVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTA"
BASE COUNT           94 a          121 c          112 g          102 t
ORIGIN      
        1 cttgaccgcc tttccgatac cgtctctgca cgcaatacct ccggattccg tgaacaggtc
       61 gctgcatggc tggaaaaact cagtgcctct gcggagcttc gacagcagtc tttcgctgtt
      121 gctgctgatg ccactgagag ctgtgaggac cgtgtcgcgc tcacatggaa caatctccgg
      181 aaaaccctcc tggtccatca ggcatcagaa ggccttttcg ataatgatac cggcgctctg
      241 ctctccctgg gcagggaaat gttccgcctc gaaattctgg aggacattgc ccgggataaa
      301 gtcagaactc tccattttgt ggatgagata gaagtctacc tggccttcca gaccatgctc
      361 gcagagaaac ttcagctctc cactgccgtg aaggaaatgc gtttctatgg cgtgtcggga
      421 gtgacagca
//
LOCUS       DADLSA010000426          415 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.432, whole genome shotgun sequence.
ACCESSION   DADLSA010000426 DADLSA010000000
VERSION     DADLSA010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.432"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..394
                     /locus_tag="JN691_004489"
     CDS             <1..394
                     /locus_tag="JN691_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY7245668.1"
                     /translation="SSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLK
                     EAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRR
                     LLERLGHTPPAEAEKAYYASIGNDDLAA"
BASE COUNT          118 a          100 c          115 g           82 t
ORIGIN      
        1 atcgtccatg gaaacgacat tcgtgctgga tgcactggag caggcgttat gggcccgtcg
       61 accgtccggc acggtccatc acagtgataa aggttctcag tatgtatcgc tggcctacac
      121 acagcggctt aaggaagccg gattactggc atcaacagga agtacaggcg actcgtatga
      181 caacgcgatg gcggagagca tcaatggtct ttacaaagcg gaggtaatac accgtaagag
      241 ctggaaaaac cgtgcagaag tggaactggc cacactcacg tgggtggact ggtataacaa
      301 tcgacgattg ctggaaaggc tgggccatac tcctccggca gaagcagaaa aagcttatta
      361 tgcttccatc ggaaacgatg atctggcagc ctgagttcac agataaaaca ctctc
//
LOCUS       DADLSA010000427          411 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.433, whole genome shotgun sequence.
ACCESSION   DADLSA010000427 DADLSA010000000
VERSION     DADLSA010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.433"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..>411)
                     /locus_tag="JN691_004490"
     CDS             complement(<1..>411)
                     /locus_tag="JN691_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAY7245669.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEA"
BASE COUNT          116 a          101 c           72 g          122 t
ORIGIN      
        1 gcttcagcta gttgcccctt aagctcttca gccataatct gacccttacc agccatctgt
       61 gataatgctg tcagtgctcg cttaatctct tcttcacttc gccccatcag acgagaatag
      121 ccaatcatat ttctaaaaag ttcttcagaa ccttgaatgc ctagagactc tctagctgaa
      181 gcaccgaaac cagcaaactg tgattgcagt tcagcgatat ctaaacccgt tgtttgagct
      241 aatgactttg tgaaatcttt tacttgattt gcatctttag aaaatacgaa atcaattgaa
      301 cgttcagcac ttgcacgttg aaggccaact tccattactc gctgataagc ggatagtaca
      361 gcctgaaagc tgataagtac acctgctgtt ttaactgctg ctgaacttaa a
//
LOCUS       DADLSA010000428          405 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.434, whole genome shotgun sequence.
ACCESSION   DADLSA010000428 DADLSA010000000
VERSION     DADLSA010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.434"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..392)
                     /locus_tag="JN691_004491"
     CDS             complement(<1..392)
                     /locus_tag="JN691_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7245670.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKRCLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSL"
BASE COUNT          107 a           76 c          115 g          107 t
ORIGIN      
        1 agtgaaacat caagagcgac caggtgaggc ggaagagccg ggagcctgct cagtctgtta
       61 tcgctggctt taagtacagt taaggacgga gggagttctg gcagagagcg tagctcattg
      121 ctagagatgt taagttcttg tatgtgcggg ggcaggtatg ggagagagcg taatctgagt
      181 aaacttaaat tgagggctgg ctcttgaaaa gccagacatc ttttcagtaa tcgaactgcc
      241 tgtgttcggt cttctgttgc agcaccttcc ttggcccagt tatcccagat gcggtgataa
      301 tccgtgatat tttgctcttc tacggataaa cgggatatac atcctaaacc acaaaaggag
      361 cggaaaaaag aaggattgtg ggcaggtttc atttattatt gatcc
//
LOCUS       DADLSA010000429          404 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.435, whole genome shotgun sequence.
ACCESSION   DADLSA010000429 DADLSA010000000
VERSION     DADLSA010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.435"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..>404)
                     /locus_tag="JN691_004492"
     CDS             complement(<1..>404)
                     /locus_tag="JN691_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245671.1"
                     /translation="PQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKL
                     LTDAVKMRGLIAPASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLN
                     AQKLRDTQHMMQQILLSLVHALYEGNPQPVFA"
BASE COUNT           93 a           91 c          112 g          108 t
ORIGIN      
        1 ggcaaaaacc ggctgcggat taccttcgta cagcgcatga acaaggctca gcagtatttg
       61 ctgcatcatg tgctgggtat cacgaagttt ttgcgcgttc aataacacga aatggctggg
      121 gcgcgtggcc cagtatgcat tgatttgcaa ctccagcata caaaccagat tgcgattaat
      181 ggtctggata ccttcatata tcgatttggg aatacgggtt tctttgctgg cgggcgcaat
      241 cagcccacgc attttcacgg catcggtcag gagtttttgt agatggcttt ccagacgtgg
      301 gcgttcgagt aagttcggtg agaatgcaga ttgatagacc cgattatact cggtcagact
      361 tttcgccagt tgaatgcgcc agtggatgaa cgcccgttgt ggcc
//
LOCUS       DADLSA010000430          392 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.436, whole genome shotgun sequence.
ACCESSION   DADLSA010000430 DADLSA010000000
VERSION     DADLSA010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.436"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..279
                     /locus_tag="JN691_004493"
     CDS             <1..279
                     /locus_tag="JN691_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7245672.1"
                     /translation="SLNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESA
                     AIIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          101 a           91 c          104 g           96 t
ORIGIN      
        1 agcctgaacg tgtactgtcg taatggccag gtggaaatcg acaacaacat cggtgaaaac
       61 gcgctgcgat cggtggcggt tggaagaaaa aattatctct tcttcggttc tgacaacggt
      121 ggagaaagcg cggcgattat ctacagtctg ctggtcacct gcaaactgaa cggagtggag
      181 ccggaggact ggttgcgcga agtgatcgtg aagctcaatg actggccgtc gaaccgagtg
      241 catgaactgc tgccctggaa cttctcagct gtaaaataat ctttccgcta cgtacttctc
      301 tgggcgctta ccactcaacg gcatcgtcaa aattatcaaa gcgcagaaca tagatttatc
      361 atgtgtttta tggatggctt tctccatcag gc
//
LOCUS       DADLSA010000431          387 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.437, whole genome shotgun sequence.
ACCESSION   DADLSA010000431 DADLSA010000000
VERSION     DADLSA010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.437"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..362)
                     /locus_tag="JN691_004494"
     CDS             complement(<1..362)
                     /locus_tag="JN691_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7245673.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYP"
BASE COUNT          113 a           80 c           68 g          126 t
ORIGIN      
        1 ttgggataca gctcattaaa tttaccgatt gccagcggta aaaaataaaa tactgcggtc
       61 ggaatacacg ctaatgtcac cataccacga tgataagcat tcatatcacg aatattaaaa
      121 agcgtttcat cgaatttctt tattaattcc cgcgcctccg ggagcaacct ttttcctgct
      181 tttgttaacg taaccttgcg agttgtacgc tcaaatagtt gaatatgtaa atcttcctcc
      241 atctttttta ttcttcgcgt taatgcaggt tgcgtaatat tgagtaattt agcggcattg
      301 ttaaatgaac tggactctgc cagtatgaca aatgccttca tacttgataa ttcatgcttc
      361 attattactc cggaaaatgg aagcgac
//
LOCUS       DADLSA010000432          385 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.438, whole genome shotgun sequence.
ACCESSION   DADLSA010000432 DADLSA010000000
VERSION     DADLSA010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.438"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            21..385
                     /locus_tag="JN691_004495"
                     /pseudo
     CDS             21..385
                     /locus_tag="JN691_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291511.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          100 a           87 c          115 g           83 t
ORIGIN      
        1 gccagtggca ggctgacgct ctgataagtg gactgaggca gcggcttgcg caatgcgggc
       61 tgcagctgca tccgcaaaaa acacgaatag tttactgtaa ggatgctgac cgacgggggg
      121 actacccgga aacgagcttc gactttctgg gctatacatt ccggccgcgt ctgtcgatga
      181 atcgctgggg taagaccttc gttaacttca gtccggcgat gagtgccaga gcaggaaaag
      241 caatccgtca ggaggtgagg agaatcgcag tgacaagtcc ttgtacgagc tggcgcatat
      301 gttcaatgcg aaaatcagag gctggataaa gtactacggt gctttttata aatcagcact
      361 gtatctgacg ctgaggcaaa tcgat
//
LOCUS       DADLSA010000433          382 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.439, whole genome shotgun sequence.
ACCESSION   DADLSA010000433 DADLSA010000000
VERSION     DADLSA010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.439"
                     /strain="HUE14"
                     /db_xref="taxon:623"
BASE COUNT          121 a           76 c          102 g           83 t
ORIGIN      
        1 agaagttctc atttgaaatg cacggtcaag caggtagcgc tctcatgact cgtcggaaga
       61 aagagaagaa ggccattaaa aaggctgagc agcttgtgaa ggatctgatt cagttcaagc
      121 tatgtgacat gggggatttc cctgatggta aaccagcatg actccgattg atttcataca
      181 taaaaatgta acaaccgagt taataaagct tgggtacgac cagaacgcgg ctatgactgg
      241 cgctgacatg gcggtggagc attaccgccg ttgttcacaa gccagcagaa aaggccgagt
      301 tttcgatgac tgcctgtaca ttgcaaaaca gtgggcaggt aggcagaaaa gggaaaaaat
      361 gacaacctga gagctacttt ca
//
LOCUS       DADLSA010000434          375 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.440, whole genome shotgun sequence.
ACCESSION   DADLSA010000434 DADLSA010000000
VERSION     DADLSA010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.440"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..>375
                     /locus_tag="JN691_004496"
     CDS             <1..>375
                     /locus_tag="JN691_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7245674.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDREALHWAVTT"
BASE COUNT           86 a           96 c          111 g           82 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggatca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aacccgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgtg aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DADLSA010000435          353 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.441, whole genome shotgun sequence.
ACCESSION   DADLSA010000435 DADLSA010000000
VERSION     DADLSA010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.441"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..347)
                     /locus_tag="JN691_004497"
     CDS             complement(<1..347)
                     /locus_tag="JN691_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245675.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RAEQEAKPFTFEW"
BASE COUNT           69 a          102 c           86 g           96 t
ORIGIN      
        1 ttccattcaa acgtgaacgg cttcgcctcc tgttctgcgc gccatgcttc aatttcctga
       61 cgcctggcct ctctggccgc ttccagcatt tctggtgtca cagtgaatgg ggctatctca
      121 ccccatttgc cgctttgcag ttcctgccag atttgctgac ccgtcggagc gacatcatca
      181 gcggtggctg tgtaggggac tgcctggtcc ctgtcgtcaa aaaaaacgtc acagtctact
      241 gcgccacttt cggtataacg gggattaatg atttttttaa tttccacggt gcattcctca
      301 cgatgtgcga ataaaaagcc cgggcattgc gccagagaca tgtgcatccg gca
//
LOCUS       DADLSA010000436          347 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.442, whole genome shotgun sequence.
ACCESSION   DADLSA010000436 DADLSA010000000
VERSION     DADLSA010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.442"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..>347)
                     /locus_tag="JN691_004498"
     CDS             complement(<1..>347)
                     /locus_tag="JN691_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY7245676.1"
                     /translation="RESDLVPSVPATSIIQDKAKKVLALKVDPESPESFMLRPKRRRW
                     VNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHDELHAD
                     TVAFEEKYGSQLEL"
BASE COUNT           81 a          102 c           80 g           84 t
ORIGIN      
        1 agctccagtt gggaaccata cttctcttca aatgccacgg tatccgcatg cagttcgtca
       61 tggtgttttc tgcacaaagg caacacaaaa aggtcatgcg cttttgtacc cattccaccc
      121 tgaccatgac caatcaggtg atgaggatcg tcggctggct taccacaaca tgcacacggc
      181 tgcgtcttaa cccagcgcgt gtacttttca ttaacccagc ggcgacgttt tgggcgtaac
      241 ataaaagact ccggcgactc cggatccact ttcagcgcca gcaccttttt cgccttatcc
      301 tggatgatgc tggtggcagg aaccgaaggc acaaggtcac tttcccg
//
LOCUS       DADLSA010000437          334 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.443, whole genome shotgun sequence.
ACCESSION   DADLSA010000437 DADLSA010000000
VERSION     DADLSA010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.443"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            1..334
                     /locus_tag="JN691_004499"
                     /pseudo
     CDS             1..334
                     /locus_tag="JN691_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           84 a           88 c          100 g           62 t
ORIGIN      
        1 accgggggcg gtgatggagg gctcaccacc gctgaacgtc agcgtctgaa agagctggag
       61 cgtgaaaatc gtgaactgcg ccgcagtaac gatatccttc gccaggcttc cgcttatttt
      121 gcgaaggcgg agttcgaccg cctctggaaa aaatgatgcc actgctggat aagctgcgtg
      181 agcagtacgg ggtcggaccg ctatgcagcg aactgcatat tgccccgtca acgtattacc
      241 actgtcagca acagcgacat catccggata aacgcagtgc ccgtgcgcag cgcgatgact
      301 ggctgaagaa agagatacag cgcgtatacg atga
//
LOCUS       DADLSA010000438          321 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.444, whole genome shotgun sequence.
ACCESSION   DADLSA010000438 DADLSA010000000
VERSION     DADLSA010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.444"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            33..311
                     /locus_tag="JN691_004500"
     CDS             33..311
                     /locus_tag="JN691_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HAY7245677.1"
                     /translation="MSDAVYRRLILMKAMSNITDCSVPDINRMLRFMFGKKRRAYVLN
                     NGGLRMSYIFESALSLAELAIIQSSGALPSPPGVYVSVVLKEPRNEGQ"
BASE COUNT           74 a           74 c           88 g           85 t
ORIGIN      
        1 acaacggaaa atcggatacc cggactgttg acctgtctga tgctgtatac cggcggctga
       61 tactgatgaa agccatgtcg aacattactg actgttccgt tccggatatt aaccggatgc
      121 tgagatttat gttcggaaaa aaacgccggg cttatgttct gaataatggt gggctgagga
      181 tgagttacat ctttgagtcc gcgctctcgt tggcagaact ggcgattatc cagtcgtcgg
      241 gtgcactgcc atccccgccg ggtgtttatg tttcagtggt tttaaaggag ccccgtaatg
      301 aaggccagtg ataacccccg c
//
LOCUS       DADLSA010000439          312 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.445, whole genome shotgun sequence.
ACCESSION   DADLSA010000439 DADLSA010000000
VERSION     DADLSA010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.445"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            24..100
                     /locus_tag="JN691_004501"
     tRNA            24..100
                     /locus_tag="JN691_004501"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="JN691_004502"
     tRNA            143..218
                     /locus_tag="JN691_004502"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           87 a           67 c           78 g           80 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aa
//
LOCUS       DADLSA010000440          311 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.446, whole genome shotgun sequence.
ACCESSION   DADLSA010000440 DADLSA010000000
VERSION     DADLSA010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.446"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..>311)
                     /locus_tag="JN691_004503"
     CDS             complement(<1..>311)
                     /locus_tag="JN691_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY7245678.1"
                     /translation="GGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHE
                     GDSIRFLYAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDCDYDGEHRDE
                     P"
BASE COUNT           80 a           88 c           71 g           72 t
ORIGIN      
        1 cggttcatcc cgatgctcac cgtcgtaatc gcaatcatat tcgtacccct ccattcgacg
       61 atacgcaaca tcatcaaggg tataaaaacc ttgtgtcgaa tagcggtaac ccattaactg
      121 aaaattggtg ccgaaggtgt tcatcgattt cgcatagagg aagcgaatgg aatccccctc
      181 atgacgactg tcgtcggcta actgactgcg cgcatgggtc acatccagcg acactgcccc
      241 ccagtttccg agattgcgcc ccagtcctaa taaaaaggcg gtgtaattgg cagataattg
      301 cgtcccgccg t
//
LOCUS       DADLSA010000441          310 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.447, whole genome shotgun sequence.
ACCESSION   DADLSA010000441 DADLSA010000000
VERSION     DADLSA010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.447"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..306
                     /locus_tag="JN691_004504"
     CDS             <1..306
                     /locus_tag="JN691_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HAY7245679.1"
                     /translation="KNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLGYHAENK
                     GLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT           87 a           67 c           72 g           84 t
ORIGIN      
        1 aagaacgggc gcttcggatt tggtcttcgg gccagcaaaa gtcgttcaac aggaagaatt
       61 ctggttctga gtctgctggc aacgttgagc actatcgtaa tgtggttact cggttatcac
      121 gctgaaaata aagggttaca cctgaaatat caggctaaca gcattaaatc ccggcgggtt
      181 atctcatatc tgacgttagc gaagaatgtt cttcgacact cgccacttat tttaagacga
      241 acagtactga gcacagttct aaaccatctg tccagaacct accgaaatat ggtgctggtt
      301 tattagcagt
//
LOCUS       DADLSA010000442          307 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.448, whole genome shotgun sequence.
ACCESSION   DADLSA010000442 DADLSA010000000
VERSION     DADLSA010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.448"
                     /strain="HUE14"
                     /db_xref="taxon:623"
BASE COUNT          102 a           61 c           47 g           97 t
ORIGIN      
        1 ccgggggatc aatcatcaat aaatatggcg ttctttttta attggctagc aacataccac
       61 ccctcatttg tatgacaaat gataatcttt catccaaact tcaaatgcaa taactaattt
      121 tatttctttc ggcatcgctc acattttatg gggaaatcga cgatcatttt tggcataaga
      181 aatccattta aaaatggcta catcccttat acctattcag agctacttat tcaggggtta
      241 ttgagatata cactgatggt gtacaaaaaa atcaccgaca ataatcacgt atcaagtaat
      301 agtcgga
//
LOCUS       DADLSA010000443          307 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.449, whole genome shotgun sequence.
ACCESSION   DADLSA010000443 DADLSA010000000
VERSION     DADLSA010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.449"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(1..241)
                     /locus_tag="JN691_004505"
                     /pseudo
     CDS             complement(1..241)
                     /locus_tag="JN691_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT          107 a           42 c           45 g          113 t
ORIGIN      
        1 tttacatcta taaaaatgtg catcatctca gatgagtata ttgaaaaaaa tgatgaagga
       61 ctggagatgg tttaggcaaa tcctgacttc tgaaaaagtc atcaaacaaa attaagaact
      121 ctgttttata ctcgcaaaac acttttacta actcattttg tcttgtttta tttattatta
      181 catcatctac tatatttgat ttataaagac aatgtggcga agttgagata ccatctgtca
      241 ttatcagtcc ttgaagttga tgaattttta ttaatttaat ttatgagtga atgtgactac
      301 tatcaaa
//
LOCUS       DADLSA010000444          303 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.450, whole genome shotgun sequence.
ACCESSION   DADLSA010000444 DADLSA010000000
VERSION     DADLSA010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.450"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..>303
                     /locus_tag="JN691_004506"
     CDS             <1..>303
                     /locus_tag="JN691_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7245680.1"
                     /translation="HDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKAR
                     SVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGEN"
BASE COUNT           91 a           63 c           95 g           54 t
ORIGIN      
        1 catgacgagg atgtgcgccg tccgacagaa atgactcagg aagcgctcag acggatagca
       61 gagttatacg acatagaagc ggagatacgt ggcagtccgg cagaggaacg gcttgcagtc
      121 agaaaagcca gaagcgtcca gttgatgcag tcgttgtacg actggataca gttgcagagg
      181 aaaacgctgt cgaaacatgc ggagatggcg aaggcgttcg actatatcct gaatcactgg
      241 aatgcgctga acgagttctg tcgtgacggc tgggtggaaa tagacaacaa catcggtgaa
      301 aac
//
LOCUS       DADLSA010000445          303 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.451, whole genome shotgun sequence.
ACCESSION   DADLSA010000445 DADLSA010000000
VERSION     DADLSA010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.451"
                     /strain="HUE14"
                     /db_xref="taxon:623"
BASE COUNT          109 a           67 c           60 g           67 t
ORIGIN      
        1 ccatggattt catagcctca cctccgcaaa taacggatgg cgtagtttta cactgagaaa
       61 tgaaagggat ttgaaaagaa aaaaacgcaa aagcgggcga aacgatatat acagtaagga
      121 aagcactcta tccaacaaac cacccacagt taatcggaat aaaagcagag tgcttatgaa
      181 tgatcgcacg cccgaaggtt agtatttctg cacagcaatt ttgcaaaaaa aggcgatcat
      241 tcataactta aacgtctttc agtcactccg ggatttccca tcatcgcaga ctgaaagact
      301 ctg
//
LOCUS       DADLSA010000446          302 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.452, whole genome shotgun sequence.
ACCESSION   DADLSA010000446 DADLSA010000000
VERSION     DADLSA010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.452"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..>302
                     /gene="virK"
                     /locus_tag="JN691_004507"
     CDS             <1..>302
                     /gene="virK"
                     /locus_tag="JN691_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY7245681.1"
                     /translation="GAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNISECL
                     AVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSDIA"
BASE COUNT           79 a           61 c           78 g           84 t
ORIGIN      
        1 ggagccccta aaaataccgg acctgatgtt attcggtgcg caacccgggc ctgttatggg
       61 attttcccaa aacggattat ttttgaagca ttttgtgcgt tgatgaaagc ttgcaatatc
      121 tctgagtgtc tggcagtcag tgagcacagt catgttttcc ggcaattgag atactggtat
      181 cagaaacgca aaacctttgt tgcggtctat agtgatttct gggagtctgt agcaggaaaa
      241 acctgtggtg actggtacag attaccaacc caggtgattc gaaagccgct aagcgatata
      301 gc
//
LOCUS       DADLSA010000447          301 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.453, whole genome shotgun sequence.
ACCESSION   DADLSA010000447 DADLSA010000000
VERSION     DADLSA010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.453"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..>301)
                     /locus_tag="JN691_004508"
     rRNA            complement(<1..>301)
                     /locus_tag="JN691_004508"
                     /product="23S ribosomal RNA"
BASE COUNT           66 a          108 c           57 g           70 t
ORIGIN      
        1 ctggaaaacc agcctacacg cttaaaccgg gacaaccgtc gcccggccaa catagccttc
       61 tccgtccccc cttcgcagta acaccaagta caggaatatt aacctgtttc ccatcgacta
      121 cgcctttcgg cctcgcctta ggggtcgact caccctgccc cgattaacgt tggacaggaa
      181 cccttggtct tccggcgagc gggcttttca cccgctttat cgttacttat gtcagcattc
      241 gcacttctga tacctccagc atacctcaca gtacaccttc acaggcttac agaacgctcc
      301 c
//
LOCUS       DADLSA010000448          297 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.454, whole genome shotgun sequence.
ACCESSION   DADLSA010000448 DADLSA010000000
VERSION     DADLSA010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.454"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            98..>297
                     /locus_tag="JN691_004509"
     CDS             98..>297
                     /locus_tag="JN691_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY7245682.1"
                     /translation="MRITTLASVVIPCLGFSASSIAATEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEAL"
BASE COUNT           74 a           61 c           70 g           92 t
ORIGIN      
        1 tcaataatct ttattttttg taaaaggcaa atatattctc attattgttt gtatttgtgt
       61 attgtcttgc cggttaacat gatcggagat tagtaatatg cgaataacca ctctggcttc
      121 cgtagtcatt ccctgtctcg gattttcagc cagcagcata gctgctacag aggatgtgat
      181 gattgtctcg gcatccggct atgagaaaaa gctgactaac gcagccgcca gtgtttctgt
      241 gattagccag gaggaattgc agtccagcca gtaccacgat ctggcggagg ctctgag
//
LOCUS       DADLSA010000449          294 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.455, whole genome shotgun sequence.
ACCESSION   DADLSA010000449 DADLSA010000000
VERSION     DADLSA010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.455"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..293)
                     /locus_tag="JN691_004510"
     CDS             complement(<1..293)
                     /locus_tag="JN691_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7245683.1"
                     /translation="MTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEGQGENG
                     DIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN"
BASE COUNT           81 a           89 c           60 g           64 t
ORIGIN      
        1 tcattatcac caagaacaat tgaattcccc cccagggcgt tctgaacgcc gatacccagc
       61 gcaccattga cctgcgaacc accgccaaca gacactttat gcgacatgga tatttcaccc
      121 gtccgcaaat taatggtgaa cgggcgcagg ggaccaatat cgccattttc cccctgacct
      181 tcactggtgg gaataaggtg caggcactct tccgaacgac gaaaaatcag gccaaaagct
      241 tcgttgaaaa tcctcagcgc attaacacca cggattttca gctctccggt tatt
//
LOCUS       DADLSA010000450          292 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.456, whole genome shotgun sequence.
ACCESSION   DADLSA010000450 DADLSA010000000
VERSION     DADLSA010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.456"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..>292
                     /locus_tag="JN691_004511"
     CDS             <1..>292
                     /locus_tag="JN691_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7245684.1"
                     /translation="DKRDLPTAEEEISRVQASIIKWYASEYYNGRQVLDEIQTPSAIN
                     SELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISV"
BASE COUNT           95 a           55 c           61 g           81 t
ORIGIN      
        1 gataagagag atttaccaac tgctgaagag gaaatatctc gcgtgcaggc gtctattatc
       61 aaatggtatg cgtcagaata ctataatggc aggcaagttc tcgatgagat acaaacgcct
      121 tcagcaatta acagtgagct ttatacaaaa atgatatacc tgaccaggaa ctggtcattg
      181 tatccgaacg gtgatggctg tgtaactata agctcaccag aaataaaaaa taaataccct
      241 gctgccattt gcctggctct gggatttttt ctaagcattg taatttctgt aa
//
LOCUS       DADLSA010000451          284 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.457, whole genome shotgun sequence.
ACCESSION   DADLSA010000451 DADLSA010000000
VERSION     DADLSA010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.457"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..258
                     /locus_tag="JN691_004512"
     CDS             <1..258
                     /locus_tag="JN691_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY7245685.1"
                     /translation="TVARQINPYIKVDIPETTRGALYSFVYNVGAGNFRTSTLLRKIN
                     QGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVCLWGQQ"
BASE COUNT           75 a           70 c           77 g           62 t
ORIGIN      
        1 actgtcgcca gacaaattaa cccgtacatc aaagtcgata taccggaaac aacgcgcggc
       61 gctctttact cgttcgttta caacgtgggc gctggcaatt tcagaacatc gacgcttctt
      121 cgcaaaataa accagggcga tatcaaaggc gcatgtgacc agctacgtcg ctggacatat
      181 gctggcggta agcaatggaa agggctgatg acccgtcgtg agattgagcg tgaagtctgt
      241 ttgtgggggc agcaatgagc agagtcaccg cgattatctc cgct
//
LOCUS       DADLSA010000452          281 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.458, whole genome shotgun sequence.
ACCESSION   DADLSA010000452 DADLSA010000000
VERSION     DADLSA010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.458"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(<1..259)
                     /locus_tag="JN691_004513"
     CDS             complement(<1..259)
                     /locus_tag="JN691_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY7245686.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIML"
BASE COUNT           82 a           65 c           77 g           57 t
ORIGIN      
        1 gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc tcaagcgcca
       61 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt agcgttacag
      121 tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc cccagagaag
      181 tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt ccaatgtgca
      241 taaaaaaatc cggaaacaag tgagcgtttc cggattctta c
//
LOCUS       DADLSA010000453          264 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.459, whole genome shotgun sequence.
ACCESSION   DADLSA010000453 DADLSA010000000
VERSION     DADLSA010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.459"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            1..264
                     /locus_tag="JN691_004514"
                     /pseudo
     CDS             1..264
                     /locus_tag="JN691_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT           71 a           70 c           63 g           60 t
ORIGIN      
        1 aacaggttgt tgatcgcggt tattctgttg ccagcgttgc aacacgtctc gatatcacca
       61 cccacagcct ttatgcctgg ataaagaagt acggtccgga ttcttccact aataaagaac
      121 agtcagatgc tcaggccgag atccgccgtc tccagaaaga gctgaaacgg gttaccgacg
      181 aacgggacat attaaaaaaa gccgcggcgt acttcgcaaa gctgtccgac tgaggtacgc
      241 ctttatccgt gacaactcct gttg
//
LOCUS       DADLSA010000454          264 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.denovo.460, whole genome shotgun sequence.
ACCESSION   DADLSA010000454 DADLSA010000000
VERSION     DADLSA010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.denovo.460"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            36..>264
                     /locus_tag="JN691_004515"
     CDS             36..>264
                     /locus_tag="JN691_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7245687.1"
                     /translation="MCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRET
                     YGTRRLQTELAENGIIVGRDRLARLRKELRLR"
BASE COUNT           76 a           60 c           74 g           54 t
ORIGIN      
        1 tcgaacaatg gcgacaacaa tttcccattg aagcgatgtg tcaggtattt ggtgtatcca
       61 ggagcggtta ttacaactgg gtacagcatg aaccctcaga cagaaaacaa agtgatgagc
      121 ggctaaaact ggagattaag gtggcacata tccgcactcg cgaaacatat ggaacccggc
      181 ggctccagac ggagctggca gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc
      241 ttcgtaagga gctaaggcta cgct
//
LOCUS       DADLSA010000455         9955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.guided.138, whole genome shotgun sequence.
ACCESSION   DADLSA010000455 DADLSA010000000
VERSION     DADLSA010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.guided.138"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            35..559
                     /locus_tag="JN691_004516"
                     /pseudo
     CDS             35..559
                     /locus_tag="JN691_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            675..1235
                     /locus_tag="JN691_004517"
                     /pseudo
     CDS             675..1235
                     /locus_tag="JN691_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1292..2749)
                     /gene="pepD"
                     /locus_tag="JN691_004518"
     CDS             complement(1292..2749)
                     /gene="pepD"
                     /locus_tag="JN691_004518"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY7245688.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3011..3469
                     /gene="gpt"
                     /locus_tag="JN691_004519"
     CDS             3011..3469
                     /gene="gpt"
                     /locus_tag="JN691_004519"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY7245689.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3561..4805
                     /gene="frsA"
                     /locus_tag="JN691_004520"
     CDS             3561..4805
                     /gene="frsA"
                     /locus_tag="JN691_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY7245690.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4863..5264
                     /locus_tag="JN691_004521"
     CDS             4863..5264
                     /locus_tag="JN691_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY7245691.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5303..6358)
                     /gene="phoE"
                     /locus_tag="JN691_004522"
     CDS             complement(5303..6358)
                     /gene="phoE"
                     /locus_tag="JN691_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY7245692.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            6646..7749
                     /gene="proB"
                     /locus_tag="JN691_004523"
     CDS             6646..7749
                     /gene="proB"
                     /locus_tag="JN691_004523"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY7245693.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7761..9014
                     /gene="proA"
                     /locus_tag="JN691_004524"
     CDS             7761..9014
                     /gene="proA"
                     /locus_tag="JN691_004524"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY7245694.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIVEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9129..9204
                     /locus_tag="JN691_004525"
     tRNA            9129..9204
                     /locus_tag="JN691_004525"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9162..9164,aa:Thr,seq:cgt)
     gene            complement(9219..9917)
                     /locus_tag="JN691_004526"
                     /pseudo
     CDS             complement(9219..9917)
                     /locus_tag="JN691_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         2382 a         2478 c         2605 g         2490 t
ORIGIN      
        1 tccctcaaag tgatctacta ttccgcgcag ctatttaggg cagtctattt tacagcggca
       61 tattgcctcg ttttcgcatc atggtttgcc tgaaggcagt gacgacgcgc tggcgtttgc
      121 gcgttttaat cgccacctga tcgctttgcg cataatgcaa caggttaagg ccaccggaag
      181 tccggttctg gatgtggcgc ataactttgt tagcgcgtgt cgaatcggtg atcaacaagg
      241 ggtcttacat cgtaaaggtg ccacgcctga tgactgtggt ctggtggtta ttcccggttc
      301 acgcggtgat tactcctggc tggttcagcc cgtcaggagt gaggaaacat tgcattcgct
      361 ggcgcatggg gctgggcgta aatgggggcg caccgagtgt aaagggcgtc tggcagcgaa
      421 atacacagcg acgcaactct cgcgtactga acttggcagt cgggtaattt gtcgcgataa
      481 acaactcatc ttcgaagaag cgccacaagc ctataaatcg gctgaaagtg tggtgcaatg
      541 tctgggcagg ccggattaat tattcctgtc gcgcggctgc gtccggtgct aacgctcaaa
      601 aacagtggag ggaaaaaagg atgatcttgc tataactctc ctcagcgcag gggccggaag
      661 aatgttgtct cgcagtgaaa aaggcgctgg acaggctaat taaagaagct gcccgacaag
      721 acgtcgcggt aacggtgctg gaaacagaaa caggccgcta ctctgacacg ctgcgttcgg
      781 cgctgatttc tctggatggc gacaacgcat gggcgttaag cgaaagctgg tgtggcacta
      841 ttcagtggat ttgcccgagc ccgtatcgcc cacatcacgg gcgtaaaaac tggtttctgg
      901 gcattgggcg ttttaccgct gatgagcagg agcaatccga tgcgatccgg tatgagacgc
      961 tgcgttcgtc ggggccgggc ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca
     1021 cgcatttggc atccggtatt agcgtgaagg ttcagtcaga gcgcagccag catgctaaca
     1081 aacggctggc gcgattgctg attgcctgga agctggatca gcagcaatag gaaaatagcg
     1141 cggtgctgaa atcacagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgcgac
     1201 gaacgtttac agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg
     1261 attaaacgcc atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt
     1321 cagtcagcag tgtccagtaa tgacctacgc tttcgatgtg aacttgctca tccggagagt
     1381 gtggaccggt gatagttggc ccgatagaaa ccatgtccat ttccggatac ggttttttga
     1441 acagaccaca ttccaggccc gcgtggataa tctggatgtt cggcgtcttg ttgaacaggc
     1501 gctgataggt ttcacgtacc agatgcatca ccggagaatt agcgtccggc tgccagccag
     1561 gatatgcgcc tttcgcttcg gttttcgcgc cagccagttt acccagcgaa tccagcatgc
     1621 tcaccacgta gtctttaccg ctgtcgatca gtgaacggat caggcagtga atttctacgt
     1681 tattgtcagt catggtcacc acaccgacgt tcagggaggt ttcgaccaca ccttttgcca
     1741 cgtcggaatt gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc
     1801 gagatttcgc aatcagggca gctttatcgt tcgctacaga gtccagcaac aaggccagat
     1861 ttttctcttt ctctgccagc tcgtttttca ggatctcctg ataggtattc accagagatt
     1921 tcagtgcgtc gactttatca gctgcgacag caatggtcgc aaaggcttca cgcgggatgg
     1981 cgttacgcag tgtgccgccg ttgaaatcga taaggcgcag gtccagttct tctgcatgac
     2041 ccgccaggaa gcgcaccagc agtttgttgg cattacccag gccaacgtgg atttcaccgc
     2101 cggagtgacc gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa
     2161 ccgcttcacg atctaaatgc aggttggagg tgaagtcgat accccccgca caacccatgt
     2221 agatttcacc ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc
     2281 tctgtaagcc gaacgcaccg tccataccgg cttcttcggt catggtcagc agcacttcca
     2341 gcgggccgtg aaccacgttt tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc
     2401 cgttatccgc acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct
     2461 ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga
     2521 ggtgggcctg taagacgacc ggtttacgat tttccatacc tgcggtagca ggtttacgaa
     2581 tcaggatatt acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa
     2641 caatgtattc agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg
     2701 caaaaatatc ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc
     2761 ttgtcgatca cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac
     2821 acaagacagg cttgcgagat atgtattgag aataccactt tatcccgcgt cagggagagg
     2881 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
     2941 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
     3001 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
     3061 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     3121 tggcggtctg gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     3181 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
     3241 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
     3301 tgttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
     3361 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggatcgaaca
     3421 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
     3481 cctggcattg ccgggcgttg ttctttttaa cttcaggcag gttacaatag tttccagtaa
     3541 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
     3601 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
     3661 ctgtgcagtc ggctcttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
     3721 tgatgtggat ctggcgcggt attgacccac gtgaaatcct cgacgtccag gcacgtattg
     3781 tgatgagcga tgctgaacgt accgacgatg atttatacga taccgtgatt ggctaccgtg
     3841 gtggcaactg gatttatgag tgggccaccc aggcgatggt gtggcaacaa aaagcctgta
     3901 cggaagaaga tccgcaactc agtggtcgac actggctgca tgcggctacg ttgtacaaca
     3961 ttgccgccta tcctcatctg aaaggagatg acctggccga gcaagcgcag gctttatcaa
     4021 accgcgccta tgaagaggcc gcccagcgtc tgccgggcac gatgcggcag atggagttta
     4081 ccgtacccgg cggtgcgccc atcaccggct ttttgcatat gccgaaaggc gatggcccgt
     4141 tcccgacagt attaatgtgt ggtggtctgg atgcgatgca gacggactat tacagcctgt
     4201 atgaacgtta ttttgcgccg cgcggcattg cgatgctgac tattgatatg ccgtcggtgg
     4261 gcttttcttc aaaatggaag ctcacccagg actccagcct gttgcatcag cacgtcttaa
     4321 aggcgctgcc taacgtaccg tgggtggatc acactcgcgt cgcggccttt ggtttccgtt
     4381 tcggcgctaa cgttgccgtg cgtctggcat accttgaatc gccgcgtctg aaagcggttg
     4441 cctgtcttgg tccggtagtt cataccctgt tgagtgattt taagtgccag caacaggtgc
     4501 cggaaatgta tcttgacgtt ctggcgagtc gtttggggat gcatgatgct tccgacgaag
     4561 cgttacgcgt ggagctgaat cgctattcat taaaagtgca aggattgctg gggcgtcgct
     4621 gcccaacgcc aatgttatca ggctactgga agaacgatcc gttcagcccg gaagaggact
     4681 cacgcttaat cacctcatca tctgctgacg gtaaattatt agagatccca tttaacccgg
     4741 tgtatcggaa ttttgacaaa ggtcttcagg aaatcaccgg ctggatcgaa aaacgcttgt
     4801 gttaaaaatt tgctaaattt tgccaatttg gtaaaacagt tgcatcacaa caggagatag
     4861 caatgacgtt accgagtgga cacccgaaga gcagattgat caaaaaattt accgcactag
     4921 gcccgtatat tcgtgaaggt aagtgcgaag ataatcgatt ctttttcgat tgtctggctg
     4981 tatgcgtcaa cgtgaaaccg gcaccggaag tgcgtgaatt ctggggctgg tggatggagc
     5041 tggaagcgca ggaatcccgt tttacctaca gctaccagtt tggtctgttc gataaagcag
     5101 gcgactggaa gagtgttccg gtaaaagata ctgaagtggt tgaacgactg gagcacaccc
     5161 tgcgtgagtt tcacgagaag ctgcgtgaac tgctgacgac gctgaatctg aagctggaac
     5221 cggcggatga ttttcgtgat gaaccggtga agttaacggc gtgagtgaaa tgtgccggat
     5281 gcatcacatc cggcaatatt cattaaaact gatacgtcat cccaaccgcg acagtatcat
     5341 cattattaat attaagctta ttatcgctat ccagttggtt gattttataa tcaacaaacg
     5401 ctgacatatt tttgttgaaa taatacgtag caccgacgtc gatataattg accagatctt
     5461 catcaccgat gccctcaata tctttccctt tcgataagac ataacccagc gatggacgca
     5521 gaccaaagtc aaactggtat tgagcgaccg cttcaaagtt ctgtgtctta ttggcaaagc
     5581 cgccagttat tggcgtcatt ttgcgtgttt cagaatagaa agttgccaga taaatattat
     5641 tggcatcgta tttcagacct gttgcccatg cttctgcacg cttgcctgtg ccacggcttt
     5701 gcaggttctg ctcgttggtg cgatctgagt tggtataggc cccactaatg gcgaaatcgc
     5761 tgccgccaaa gtcatatgtc aatgacgtgc cgaagccatc gccgttttgc tttttaacgt
     5821 cgcggttttc gtttttccct tgatattgca gggttaagtt caggccatcg ataacgccga
     5881 agaagtcggt gttccgatac gtcgccagac cgctggcgcg tttggtcata aagttgtcgg
     5941 tctgcgccga ggaatcgcca ccaaattccg ggaacatatc ggtccaggct tccacgtcat
     6001 acaacgcgcc caggttacga ccataatcga aagaacccaa atctttatat ttcaacccgg
     6061 caaaagcgag acgcgttttt tgctgtgcag tatcactctc cgctttattt ccggcaaact
     6121 ccgcttccca ccgaccataa ccagtcagtt gatcgttaat ttgagtttca cctttaaaac
     6181 caaaacggat ataactctgg tcgccatctt tactggcgtt atcactcata taatgcatgg
     6241 ctttaacttt gccatagaca tccagtttat taccgtcttt attatatatt tctgcagcct
     6301 gtacagatgc agatgccaca atgcccatca ccactaatgc cagagtgctc tttttcattt
     6361 tcattcctga ttttaattaa cgcgcgaata ttcagcggga gagccccgtt gaaaacagga
     6421 aagtttttaa cctgagattg ttaaagatat attacagatt aatgatattc tgaaaatgtg
     6481 gtaatttatt aaatctgtaa taaaagcgta aacaactgcc gctacgcttg ctgatcccgc
     6541 gcaacaaaac gccatgcttt gctcgcagat ggttggcaac cgacgacagt cctgctaaaa
     6601 cgttcgtttg atatcatttt tcctaaaatt gaatggcaga gaatcatgag tgacagccag
     6661 acgctggtgg taaaactcgg caccagtgtg ctaacaggcg gatcgcgccg tctgaaccgt
     6721 gcccatatcg ttgaacttgt tcgccagtgc gcgcagttac atgccgccgg gcatcggatt
     6781 gttattgtga cgtcgggcgc gatcgccgcc ggacgtgagc acctgggtta cccggaactg
     6841 ccagcgacta tcgcctcgaa acaactgctg gcggcggtag ggcagagtcg actgattcaa
     6901 ctgtgggaac agctgttttc gatttatggc attcacgtcg ggcaaatgct gctgacccgt
     6961 gctgatatgg aagaccgtga acgcttcctg aacgcccgcg acaccctgcg tgcgttgctc
     7021 gataacaata tcgttccggt aatcaatgag aacgatgctg tcgctacggc agagattaag
     7081 gtcggcgata acgataacct ttctgcgctg gcggcgattc tggcgggtgc cgataaactg
     7141 ttgctgctga ccgatcaaaa aggtttgtat accgctgatc cgcgcagcaa tccgcaggca
     7201 gaactgatta aagatgttta cggcattgat gacgcactgc gcgcgattgc cggtgacagc
     7261 gtttcaggcc tcggaactgg cggcatgagt accaaattgc aggccgctga cgtggcttgc
     7321 cgtgcgggta tcgacaccat tattgccgcg ggcagcaagc cgggcgttat tggtgatgtg
     7381 atggaaggca tttccgtcgg tacgctgttc catgcccagg cgactccgct tgaaaaccgt
     7441 aaacgctgga ttttcggtgc gccgccggcg ggtgaaatca cggtagatga aggggcaact
     7501 gccgccattc tggaacgcgg cagctccctg ttgccgaaag gcattaaaag cgtgactggc
     7561 aatttctcgc gtggtgaagt catccgcatt tgcaacctcg aaggtcgcga tatcgcccac
     7621 ggtgtcagtc gttacaacag cgatgcatta cgccgtattg ccggacacca ctcgcaagaa
     7681 attgatgcaa tactgggata tgaatacggc ccggttgccg ttcaccgtga tgacatgatt
     7741 acccgttaag gagctggccg atgctggaac aaatgggcat tgccgcgaag caagcctcgt
     7801 ataaattagc gcaactctcc agccgcgaaa aaaatcgcgt gctggaaaaa atcgccgatg
     7861 aactggaagc acaaagcgaa atcatcctca acgctaacgc ccaggatgtt gctgacgcgc
     7921 gagccaatgg ccttagcgaa gcgatgcttg accgtctggc actgacgccc gcacggctga
     7981 aaggcattgc cgacgatgta cgtcaggtgt gcaacctcgc cgatccggtg gggcaggtaa
     8041 tcgatggcgg cgtactggac agcggcctgc gtcttgagcg tcgtcgcgta ccgctggggg
     8101 ttattggcgt gatttatgaa gcgcgcccga acgtgacggt tgatgtcgct tcgctgtgcc
     8161 tgaaaaccgg taatgcggtg atcctgcgtg gtggtaaaga aacctgtcgc actaacgcgg
     8221 caacggtggc ggtgattcag gacgccctga aatcctgtgg cttaccggcg ggtgccgtgc
     8281 aggcgattga taatcctgac cgtgcgctgg tcagtgaaat gctgcgtatg gataaataca
     8341 tcgacatgct gatcccgcgt ggtggcgctg gtttgcataa actgtgccgt gaacagtcga
     8401 caatcccggt gatcacaggt ggtataggcg tatgccatat ttacgttgat gaaagtgcag
     8461 agatcgttga agcattaaaa gtgatcgtca atgcgaaaac tcagcgtccg agcacatgta
     8521 atacggttga aacgttgctg gtgaataaaa acatcgccga tagcttcctg cccgcattaa
     8581 gcaaacaaat ggcggaaagc ggcgtgacat tacacgcaga tgcagctgcg ctgacacagt
     8641 tgcaggcagg ccctgcgaag gtggtggcgg ttaaagccga agagtatgac gatgagtttc
     8701 tgtcattaga tttgaacgtc aaaatcgtca gcgatcttga cgatgccatc gcccatattc
     8761 gtgaacacgg cacacaacac tccgatgcga tcctgacccg cgatatgcgc aacgcccagc
     8821 gttttgttaa cgaagtggat tcgtccgctg tttacgttaa cgcctctacg cgttttaccg
     8881 acggcggcca gtttggtctg ggtgcggaag tggcggtaag cacacaaaaa ctccacgcgc
     8941 gtggcccaat ggggctggaa gcactgacca cttacaagtg gatcggcatt ggtgattaca
     9001 ccattcgtgc gtaaataaaa ccgggtgatg caaaagtagc catttgattc acaaggccat
     9061 tgacgcatcg cccggttagt tttaaccttg tccaccgtga ttcacgttcg tgaacatgtc
     9121 ctttcagggc cgatatagct cagttggtag agcagcgcat tcgtaatgcg aaggtcgtag
     9181 gttcgactcc tattatcggc accattaaaa tcaataagtt acacatcatt agtaccttcc
     9241 ttattttttg actgggacaa atttgggacc gatgggttca ggatcgagtc tatttgccgt
     9301 gcgtgttcgg taaggtgatt aggtgcaagg tgagcatatc gacgaaccat ttcgatagac
     9361 tcccagcctc ccatttcctg taacactgac aacgggactc cggcttgaac cagccaactt
     9421 gcccaggtgt gtctcaagtc gtgaaatctg aaatcatcaa taccagcccg tctcagcgcc
     9481 gctttccagg ctgtgtttgc gtcataccgc atcttcctta ctgttggcgc tttcgttccg
     9541 tctggtttgg tacagctttc cttgtacaca aatacccaac ggtgatgatt cccgatttgt
     9601 tttttcaaaa cgcgacatgc agtatcattc agcgcaacgc cgattgcgcg gtttgattta
     9661 ctctcttccg ggtttatcca tgccacccgg cgctgcatat ctatttgttg ccattcaagg
     9721 ttgatgatgt tcgagcgtct taagcctgtt gccagtgcaa attcaacaac agactttaat
     9781 ggctccggac attcatcaat cagcctttgt gcttcatggg gctccagcca gcggatccgt
     9841 ttattctttg gttgaggcac tttaataatt ggtgccttat ccagcatttt ccattcacgc
     9901 tctgcggctc ttagtagtaa tgccagtcag ttaagcaact gactggctct ttttc
//
LOCUS       DADLSA010000456         4469 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.guided.233, whole genome shotgun sequence.
ACCESSION   DADLSA010000456 DADLSA010000000
VERSION     DADLSA010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.guided.233"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            62..304
                     /locus_tag="JN691_004527"
     CDS             62..304
                     /locus_tag="JN691_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HAY7245695.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLMRWKGGGDVVYMALL"
     gene            complement(336..986)
                     /gene="tetR(A)"
                     /locus_tag="JN691_004528"
     CDS             complement(336..986)
                     /gene="tetR(A)"
                     /locus_tag="JN691_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HAY7245696.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1092..2291
                     /gene="tet(A)"
                     /locus_tag="JN691_004529"
     CDS             1092..2291
                     /gene="tet(A)"
                     /locus_tag="JN691_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HAY7245697.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2323..3207)
                     /locus_tag="JN691_004530"
     CDS             complement(2323..3207)
                     /locus_tag="JN691_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAY7245698.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(3345..3737)
                     /locus_tag="JN691_004531"
     CDS             complement(3345..3737)
                     /locus_tag="JN691_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAY7245699.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            3741..4469
                     /locus_tag="JN691_004532"
                     /pseudo
     CDS             3741..4469
                     /locus_tag="JN691_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          861 a         1425 c         1376 g          807 t
ORIGIN      
        1 ttagcgtacg attttttccg aattctgcgg gctcccctat ctcatctgcg caaggcagaa
       61 cgtgaagacg gccgccctgg acctcgcccg cgagcgccag gcgcacgagg ccggcgcgcg
      121 gacccgcgcc acggcccacg agcggacgcc gcagcaggag cgccagaagg ccgccagaga
      181 ggccgagcgc ggccgtgagg cttggacgct agggcagggc atgaaaaagc ccgtagcggg
      241 ctgctacggg cgtctgatgc ggtggaaagg gggaggggat gttgtctaca tggctctgct
      301 gtagtgagtg ggttgcgctc cggcagcggt cctgatcaat cgtcaccctt tctcggtcct
      361 tcaacgttcc tgacaacgag cctccttttc gccaatccat cgacaatcac cgcgagtccc
      421 tgctcgaacg ctgcgtccgg accggcttcg tcgaaggcgt ctatcgcggc ccgcaacagc
      481 ggcgagagcg gagcctgttc aacggtgccg ccgcgctcgc cggcatcgct gtcgccggcc
      541 tgctcctcaa gcacggcccc aacagtgaag tagctgattg tcatcagcgc attgacggcg
      601 tccccggccg aaaaacccgc ctcgcagagg aagcgaagct gcgcgtcggc cgtttccatc
      661 tgcggtgcgc ccggtcgcgt gccggcatgg atgcgcgcgc catcgcggta ggcgagcagc
      721 gcctgcctga agctgcgggc attcccgatc agaaatgagc gccagtcgtc gtcggctctc
      781 ggcaccgaat gcgtatgatt ctccgccagc atggcttcgg ccagtgcgtc gagcagcgcc
      841 cgcttgttcc tgaagtgcca gtaaagcgcc ggctgctgaa cccccaaccg ttccgccagt
      901 ttgcgtgtcg tcagaccgtc tacgccgacc tcgttcaaca ggtccagggc ggcacggatc
      961 actgtattcg gctgcaactt tgtcatgctt gacactttat cactgataaa cataatatgt
     1021 ccaccaactt atcagtgata aagaatccgc gcgttcaatc ggaccagcgg aggctggtcc
     1081 ggaggccaga cgtgaaaccc aacagacccc tgatcgtaat tctgagcact gtcgcgctcg
     1141 acgctgtcgg catcggcctg attatgccgg tgctgccggg cctcctgcgc gatctggttc
     1201 actcgaacga cgtcaccgcc cactatggca ttctgctggc gctgtatgcg ttgatgcaat
     1261 ttgcctgcgc acctgtgctg ggcgcgctgt cggatcgttt cgggcggcgg ccggtcttgc
     1321 tcgtctcgct ggccggcgct gctgtcgact acgccatcat ggcgacggcg cctttccttt
     1381 gggttctcta tatcgggcgg atcgtggccg gcatcaccgg ggcgactggg gcggtagccg
     1441 gcgcttatat tgccgatatc actgatggcg atgagcgcgc gcggcacttc ggcttcatga
     1501 gcgcctgttt cgggttcggg atggtcgcgg gacctgtgct cggtgggctg atgggcggtt
     1561 tctcccccca cgctccgttc ttcgccgcgg cagccttgaa cggcctcaat ttcctgacgg
     1621 gctgtttcct tttgccggag tcgcacaaag gcgaacgccg gccgttacgc cgggaggctc
     1681 tcaacccgct cgcttcgttc cggtgggccc ggggcatgac cgtcgtcgcc gccctgatgg
     1741 cggtcttctt catcatgcaa cttgtcggac aggtgccggc cgcgctttgg gtcattttcg
     1801 gcgaggatcg ctttcactgg gacgcgacca cgatcggcat ttcgcttgcc gcatttggca
     1861 ttctgcattc actcgcccag gcaatgatca ccggccctgt agccgcccgg ctcggcgaaa
     1921 ggcgggcact catgctcgga atgattgccg acggcacagg ctacatcctg cttgccttcg
     1981 cgacacgggg atggatggcg ttcccgatca tggtcctgct tgcttcgggt ggcatcggaa
     2041 tgccggcgct gcaagcaatg ttgtccaggc aggtggatga ggaacgtcag gggcagctgc
     2101 aaggctcact ggcggcgctc accagcctga cctcgatcgt cggacccctc ctcttcacgg
     2161 cgatctatgc ggcttctata acaacgtgga acgggtgggc atggattgca ggcgctgccc
     2221 tctacttgct ctgcctgccg gcgctgcgtc gcgggctttg gagcggcgca gggcaacgag
     2281 ccgatcgctg atcgtggaaa cgataggcct atgccatgcg ggtcaaggcg acttccggca
     2341 agctatacgc gccctaggag tgcggttgga acgttggccc agccagatac tcccgatcac
     2401 gagcaggacg ccgatgattt gaagcgcact cagcgtctga tccaagaaca accatcctag
     2461 caacacggcg gtccccgggc tgagaaagcc cagtaaggaa acaactgtag gttcgagtcg
     2521 cgagatcccc cggaaccaaa ggaagtaggt taaacccgct ccgatcaggc cgagccacgc
     2581 caggccgaga acattggttc ctgtaggcat cgggattggc ggatcaaaca ctaaagctac
     2641 tggaacgagc agaagtcctc cggccgccag ttgccaggcg gtaaaggtga gcagaggcac
     2701 gggaggttgc cacttgcggg tcagcacggt tccgaacgcc atggaaaccg cccccgccag
     2761 gcccgctgcg acgccgacag gatctagcgc tgcgtttggt gtcaacacca acagcgccac
     2821 gcccgcagtt ccgcaaatag cccccaggac cgccatcaat cgtatcgggc tacctagcag
     2881 agcggcagag atgaacacga ccatcagcgg ctgcacagcg cctaccgtcg ccgcgacccc
     2941 gcccggcagg cggtagaccg aaataaacaa caagctccag aatagcgaaa tattaagtgc
     3001 gccgaggatg aagatgcgca tccaccagat tcccgttgga atctgtcgga cgatcatcac
     3061 gagcaataaa cccgccggca acgcccgcag catcgcgacc gtcatcggtg agaagttcgg
     3121 caggtattgg gtggtgacaa tgtaggtgct gccccaaatg gcaggtgcta tcgctgtgaa
     3181 caataaatcg ggcgtgcgta atgacatgcg attacaagac ctccgctacg gcgatgggtt
     3241 tttgagagtt gcaggcgccg cgatgaccgc accctgactt ggactggccc tgcctcgcac
     3301 caatccacct gcggctatgg ccgcgatcat gcgaggcccg aaaattacac gacctgtaca
     3361 cgtccgccga acccggaacg caacagagct tcgtgggcat cccgatccgg gtcgtagcag
     3421 cagtcctgga ccaaacgcac gtcgtagtcc gcatcactag cccaggcgac gcttgaaaga
     3481 acaacgccgg tggtgcttat cccggccatg acaagcgtgc ttacgccgcg cgtccgaagg
     3541 tcggcgtcga gcgccgtgcc ataaaagacg ctggctcgcg gacatgcata gaaaaggtcg
     3601 ccccgttcga tcgcaagccc ttcgacgggc agaccggtgc gaaatcgtcc gctcgggaga
     3661 tacggtgaga tttggcgatt cgtcgccggc ggcgcatgtt catactcttc gccgagcgaa
     3721 aagttgggga acagcacggg ccggccgcgc aaggcgtgct ggcagccgtg cagaccctgc
     3781 gtgagatgaa cgccgacaac ctgcgcaagg tgccggccga tgcacccacg gccttcatca
     3841 agccgcgctg gaagccgctg gtgatcaccc cggaaggcct cgaccggaaa ttctacgaaa
     3901 tctgcgccct gtccgagctg aagaacgccc tgcgctccgg cgacatctgg gtcaagggct
     3961 cgcggcagtt ccgcgacttc gacgactacc tgctgccggc cgagaagttc gccgcactca
     4021 agcgcgagca ggccctgccc ctggcgatca acccgaacag cgaccagtac ctggaagagc
     4081 gtttgcagct gctggacgag cagttggcca ccgtcacccg cctggccaag gacaacgagc
     4141 tgcccgatgc catcctcacc gagtcagggc tgaaaatcac cccgctggat gcggcggtgc
     4201 cggatcgggc gcaggcgctg atcgaccaaa ccagccagtt actgccgcgc atcaagatca
     4261 ccgaactgct gatggacgtg gacgactgga cgggcttcag ccgccacttc acccacttga
     4321 aggacggggc cgaggccaaa gacaggacgt tgctgctgtc cgcaatcctc ggtgatgcga
     4381 tcaacctcgg gctgaccaag atggccgagt cgagccccgg cctgacctac gccaagctgt
     4441 cctggctgca agcctggcac atccgcgac
//
LOCUS       DADLSA010000457         3970 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.guided.245, whole genome shotgun sequence.
ACCESSION   DADLSA010000457 DADLSA010000000
VERSION     DADLSA010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.guided.245"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            <1..283
                     /locus_tag="JN691_004533"
     CDS             <1..283
                     /locus_tag="JN691_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY7245700.1"
                     /translation="FAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLLVQCARVFIQ
                     KLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA"
     gene            complement(465..641)
                     /locus_tag="JN691_004534"
     CDS             complement(465..641)
                     /locus_tag="JN691_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HAY7245701.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            complement(870..947)
                     /locus_tag="JN691_004535"
                     /pseudo
     CDS             complement(870..947)
                     /locus_tag="JN691_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            971..1786
                     /gene="sul2"
                     /locus_tag="JN691_004536"
     CDS             971..1786
                     /gene="sul2"
                     /locus_tag="JN691_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAY7245702.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1847..2650
                     /locus_tag="JN691_004537"
                     /note="aph(3'')-Ib"
     CDS             1847..2650
                     /locus_tag="JN691_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAY7245703.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2650..3486
                     /locus_tag="JN691_004538"
     CDS             2650..3486
                     /locus_tag="JN691_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAY7245704.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3458..3871)
                     /locus_tag="JN691_004539"
     CDS             complement(3458..3871)
                     /locus_tag="JN691_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001945043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7245705.1"
                     /translation="MAEQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVA
                     FSCKRRGFCPSCGARRMAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFGVVCGKGRN
                     PTLRLWPAIFSGEIDVFPGDRRSRLISSMTSVAPA"
BASE COUNT          864 a         1085 c         1094 g          927 t
ORIGIN      
        1 ctttgcggcg gcaacagggc tggtaccccg acagtacagc acgggaggtc ggacgacatt
       61 gttagggatt agcaagcggg gcaacaaaaa gatccgaact ttgttggttc agtgtgccag
      121 ggtattcata caaaaactgg aacaccagtc tggcaagttg gccgactggg tcagggagtt
      181 gttgtgtcgg aaaagcaact ttgtcgtcac ctgtgctctg gcaaacaagc tggccagaat
      241 agcctgggca ctgacggcgc gacagcaaac ttacgaagca taaaggcaga aatacaccag
      301 tttaaacaat cattcatctg gttttgcgaa tactgatatt gatgatacta acggcccacc
      361 ggcctgttga ggaacctgta aaacggaaag gctcattgaa gccgtatatt ttctggaggt
      421 tcatcaggcg cggaactcat cgaggcgcgg gaataaaatc ccattcagac gccggataga
      481 ttcaagcaag ccaacttgtc gtcaaaatcg gtgttgcaaa aacgggagtg accatagatt
      541 ccgttttctg aggcgacccc cacatagaga ccttcccggt cattcacttt gtagagtttg
      601 tccctgggct tgagattgcg cagcttggta tcggtcagca tgtaccttgt ccttcttcgt
      661 ctccgatacc cactctattg taaacaagac atttttatct tttatattca atggcttatt
      721 ttcctgctaa ttggtaatac catgaaaaat accatgctca gaaaaggctt aacaatattt
      781 tgaaaaattg cctactgagc gctgccgcac agctccatag gccgctttcc tggctttgct
      841 tccagatgta tgctattctg ctcctgcagc taatggagca ccgcaaacag gttactcgcc
      901 tggggattcc ctttcgaccc gagcatccgt atgagactca tgctcgatta ttattattat
      961 agaagccccc atgaataaat cgctcatcat tttcggcatc gtcaacataa cctcggacag
     1021 tttctccgat ggaggccggt atctggcgcc agacgcagcc attgcgcagg cgcgtaagct
     1081 gatggccgag ggggcagatg tgatcgacct cggtccggca tccagcaatc ccgacgccgc
     1141 gcctgtttcg tccgacacag aaatcgcgcg tatcgcgccg gtgctggacg cgctcaaggc
     1201 agatggcatt cccgtctcgc tcgacagtta tcaacccgcg acgcaagcct atgccttgtc
     1261 gcgtggtgtg gcctatctca atgatattcg cggttttcca gacgctgcgt tctatccgca
     1321 attggcgaaa tcatctgcca aactcgtcgt tatgcattcg gtgcaagacg ggcaggcaga
     1381 tcggcgcgag gcacccgctg gcgacatcat ggatcacatt gcggcgttct ttgacgcgcg
     1441 catcgcggcg ctgacgggtg ccggtatcaa acgcaaccgc cttgtccttg atcccggcat
     1501 ggggtttttt ctgggggctg ctcccgaaac ctcgctctcg gtgctggcgc ggttcgatga
     1561 attgcggctg cgcttcgatt tgccggtgct tctgtctgtt tcgcgcaaat cctttctgcg
     1621 cgcgctcaca ggccgtggtc cgggggatgt cggggccgcg acactcgctg cagagcttgc
     1681 cgccgccgca ggtggagctg acttcatccg cacacacgag ccgcgcccct tgcgcgacgg
     1741 gctggcggta ttggcggcgc tgaaagaaac cgcaagaatt cgttaactgc acattcggga
     1801 tatttctcta tattcgcgct tcatcagaaa actgaaggaa cctccattga atcgaactaa
     1861 tatttttttt ggtgaatcgc attctgactg gttgcctgtc agaggcggag aatctggtga
     1921 ttttgttttt cgacgtggtg acgggcatgc cttcgcgaaa atcgcacctg cttcccgccg
     1981 cggtgagctc gctggagagc gtgaccgcct catttggctc aaaggtcgag gtgtggcttg
     2041 ccccgaggtc atcaactggc aggaggaaca ggagggtgca tgcttggtga taacggcaat
     2101 tccgggagta ccggcggctg atctgtctgg agcggatttg ctcaaagcgt ggccgtcaat
     2161 ggggcagcaa cttggcgctg ttcacagcct atcggttgat caatgtccgt ttgagcgcag
     2221 gctgtcgcga atgttcggac gcgccgttga tgtggtgtcc cgcaatgccg tcaatcccga
     2281 cttcttaccg gacgaggaca agagtacgcc gcagctcgat cttttggctc gtgtcgaacg
     2341 agagctaccg gtgcggctcg accaagagcg caccgatatg gttgtttgcc atggtgatcc
     2401 ctgcatgccg aacttcatgg tggaccctaa aactcttcaa tgcacgggtc tgatcgacct
     2461 tgggcggctc ggaacagcag atcgctatgc cgatttggca ctcatgattg ctaacgccga
     2521 agagaactgg gcagcgccag atgaagcaga gcgcgccttc gctgtcctat tcaatgtatt
     2581 ggggatcgaa gcccccgacc gcgaacgcct tgccttctat ctgcgattgg accctctgac
     2641 ttggggttga tgttcatgcc gcctgttttt cctgctcatt ggcacgtttc gcaacctgtt
     2701 ctcattgcgg acaccttttc cagcctcgtt tggaaagttt cattgccaga cgggactcct
     2761 gcaatcgtca agggattgaa acctatagaa gacattgctg atgaactgcg cggggccgac
     2821 tatctggtat ggcgcaatgg gaggggagca gtccggttgc tcggtcgtga gaacaatctg
     2881 atgttgctcg aatatgccgg ggagcgaatg ctctctcaca tcgttgccga gcacggcgac
     2941 taccaggcga ccgaaattgc agcggaacta atggcgaagc tgtatgccgc atctgaggaa
     3001 cccctgcctt ctgcccttct cccgatccgg gatcgctttg cagctttgtt tcagcgggcg
     3061 cgcgatgatc aaaacgcagg ttgtcaaact gactacgtcc acgcggcgat tatagccgat
     3121 caaatgatga gcaatgcctc ggaactgcgt gggctacatg gcgatctgca tcatgaaaac
     3181 atcatgttct ccagtcgcgg ctggctggtg atagatcccg tcggtctggt cggtgaagtg
     3241 ggctttggcg ccgccaatat gttctacgat ccggctgaca gagacgacct ttgtctcgat
     3301 cctagacgca ttgcacagat ggcggacgca ttctctcgtg cgctggacgt cgatccgcgt
     3361 cgcctgctcg accaggcgta cgcttatggg tgcctttccg cagcttggaa cgcggatgga
     3421 gaagaggagc aacgcgatct agctatcgcg gccgcgatca agcaggtgcg acagacgtca
     3481 tactagatat caagcgactt ctcctatccc ctgggaacac atcaatctca ccggagaata
     3541 tcgctggcca aagccttagc gtaggattcc gccccttccc gcaaacgacc ccaaacagga
     3601 aacgcagctg aaacgggaag ctcaacaccc actgacgcat gggttgttca ggcagtactt
     3661 catcaaccag caaggcggca ctttcggcca tccgccgcgc cccacagctc gggcagaaac
     3721 cgcgacgctt acagctgaaa gcgaccaggt gctcggcgtg gcaagactcg cagcgaaccc
     3781 gtagaaagcc atgctccagc cgcccgcatt ggagaaattc ttcaaattcc cgttgcacat
     3841 agcccggcaa ttcctttccc tgctctgcca taagcgcagc gaatgccggg taatactcgt
     3901 caacgatctg atagagaagg gtttgctcgg gggcactgtt gcaaagttag cgatgaggca
     3961 gccttttgtc
//
LOCUS       DADLSA010000458         2554 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.guided.284, whole genome shotgun sequence.
ACCESSION   DADLSA010000458 DADLSA010000000
VERSION     DADLSA010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.guided.284"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(38..469)
                     /locus_tag="JN691_004540"
                     /pseudo
     CDS             complement(38..469)
                     /locus_tag="JN691_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
     gene            633..1190
                     /locus_tag="JN691_004541"
     CDS             633..1190
                     /locus_tag="JN691_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7245706.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            1373..2233
                     /locus_tag="JN691_004542"
     CDS             1373..2233
                     /locus_tag="JN691_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAY7245707.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          691 a          580 c          648 g          635 t
ORIGIN      
        1 acaaaaggct gcctcatcgc taactttgca acagtgccag gccgggtcgc tcattactga
       61 gccatgcgcg ggtgtacagc aggcgcttca ggcggaaaga ccaggggaaa tcaccaaatt
      121 catggtaacc gtaatattcc ttaatcagcg cagtatgctc tctgagggtg gtaacccgtt
      181 ctgcgtagcg ggtaaggact tccggacggc gaatattaag ctgcaccgcg acaaattgct
      241 gaacacctgg cagaacccgg gttaagtctg tcagaaaggt gcccagaaaa cgggcggtgg
      301 tgagctgaag cgcaattccc agccggttat gccttccccg ccgctggtta atgaaggcaa
      361 gatcccgctc atcaagatga aaatagcgcg ccagctgcac gtcattgggt tctgcaacat
      421 agcgaccata attcagttcc tgctcagtgg tcagaaaatc gacgggcata ccggcctccc
      481 tgcctgatgg tcatgaatta acaatttcgc aaccgtccga aatattataa attatcggac
      541 atcgtaaaac tgttacatta atatgtctat taaatcgtaa atttgtaata atagacatga
      601 gttgtccgat attcgattta aggtacattt ttatgcgact ttttggttac gctcgggtct
      661 caaccagtca gcagtctctt gatcttcagg tcagagcact caaagacgca ggtgtgaaag
      721 caaaccgtat atttaccgat aaggcatccg gcagttcaac agaccgggaa gggctggatt
      781 tgctgaggat gaaggtggag gaaggtgatg tcattctggt taagaagctc gaccgtcttg
      841 gccgcgacac tgccgatatg atccaactga taaaggaatt tgacgctcag ggcgtggcag
      901 tccggttcat tgatgacggg atcagtaccg acggtgatat ggggcaaatg gtggtcacca
      961 tcctgtcggc tgtggcacag gctgaacgcc ggaggatcct agaacgcacg aatgagggcc
     1021 gacaggaagc aaagctgaaa ggaatcaaat ttggccgcag gcgtaccgtg gacaggaacg
     1081 tcgtgctgac gcttcatcag aagggcactg gtgcaacgga aattgctcat cagctcagta
     1141 ttgcccgctc cacggtttat aaaattcttg aagacgaaag ggcctcgtga tacgcctatt
     1201 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     1261 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1321 catgagacaa taaccctggt aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1381 tcaacatttt cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1441 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     1501 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     1561 ttttccaatg atgagcactt ttaaagttct gctatgtggt gcggtattat cccgtgttga
     1621 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     1681 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     1741 tgccataacc atgagtgata acactgctgc caacttactt ctgacaacga tcggaggacc
     1801 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     1861 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     1921 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     1981 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2041 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2101 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2161 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2221 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2281 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2341 cccttaacgt gagttttcgt tccactgagc gtcagacccc taaaagaact ttccgctaag
     2401 cgatagactg tatgtaaaca cagtattgca aggacgcgga acatgcctca tgtggcggcc
     2461 aggacggcca gccgggatcg ggatactggt cgttaccaga gccaccgacc cgagcaggca
     2521 ctgttgcaaa tagtcggtgg tgataaactt atca
//
LOCUS       DADLSA010000459         2472 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.guided.288, whole genome shotgun sequence.
ACCESSION   DADLSA010000459 DADLSA010000000
VERSION     DADLSA010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.guided.288"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            26..1159
                     /gene="blaEC"
                     /locus_tag="JN691_004543"
     CDS             26..1159
                     /gene="blaEC"
                     /locus_tag="JN691_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY7245708.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1248..1781
                     /locus_tag="JN691_004544"
     CDS             1248..1781
                     /locus_tag="JN691_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY7245709.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1778..2095)
                     /locus_tag="JN691_004545"
     CDS             complement(1778..2095)
                     /locus_tag="JN691_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY7245710.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2271..2417)
                     /gene="ecnB"
                     /locus_tag="JN691_004546"
     CDS             complement(2271..2417)
                     /gene="ecnB"
                     /locus_tag="JN691_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY7245711.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
BASE COUNT          632 a          650 c          612 g          578 t
ORIGIN      
        1 ccgttttgta tggaaaccag accttatgtt caaaacgacg ctctgcgcct tattaattac
       61 cgcctcttgc tccacatttg ctgcccctca acaaatcaac gatattgtgc atcgcacaat
      121 taccccgctt atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg
      181 taaaccttat tactttacct ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca
      241 gcaaacgttg tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga
      301 cgctattgct cgaggggaaa tcaagttaag cgatcccgca acaaaatact ggcctgaact
      361 taccgctaaa cagtggaatg ggatcacact attacatctt gcgacctaca ccgctggcgg
      421 cctgccattg caggtgccgg atgaagtgaa atcctcaagc gacttgctgc gcttctatca
      481 aaactggcag cctgcatggg ctccaggaac acaacgtctg tatgccaact ccagtatcgg
      541 tttgttcggc gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac
      601 tcgtgtcttc cagccactca aactcaacca tacgtggatt aatgtacctc ccccagaaga
      661 aaagaattac gcctggggat atcgcgaagg taaggcagtg catgtttcgc caggggcgtt
      721 agatgctgaa gcttatggtg tgaagtcgac cattgaagat atggcccgct gggtgcaaag
      781 caatttaacc ccccttgata tcaatgagaa aacgcttcaa caagggatac aactggcaca
      841 atctcgctac tggcaaaccg gcgatatgta tcagggcctg ggctgggaaa tgctggactg
      901 gccggtcaat cctgacagca tcattaacgg cagtgacaat aaaattgcac tggcagcacg
      961 cccggtaaaa gcgattaagc ccccaactcc tgcagtacgc gcatcatggg tacataaaac
     1021 gggggcgacc ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag agctgggtat
     1081 cgtgatgctg gctaacaaaa actatcccaa tccagcgaga gtcgccgccg cctgtcagat
     1141 tctcaacgct ctacagtaaa atttcatcgg gtccgaattt tcggaccttt tctgcgcttt
     1201 tccttgctgt catctacact tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc
     1261 ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc ctgtagttct cctacgccgc
     1321 cgcgtggcgt gaccgtagtg aataatttcg acgccaaacg ttatctcggt acctggtatg
     1381 agattgcccg ttttgatcac cgttttgaac gtggactgga aaaagtcacc gcaacataca
     1441 gcctgcgtga tgacggcggc ctgaatgtca ttaataaagg ctataaccct gacagaggaa
     1501 tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc accaactcgc gctgcgctga
     1561 aagtgtcatt ctttggtcct ttctatggcg gttataacgt tattgcactc gatcgggaat
     1621 accgccatgc gctggtttgc ggcccggacc gcgactacct gtggatactc tcccgcacgc
     1681 caaccatttc tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg gaagggtttg
     1741 atgtcagtaa gtttatttgg gtacagcagc ccggtagtta gtgagtgctg agtttcagac
     1801 caataatccc caatacgatt aacgccagac tcgccagacg catcgggtta gcggactcac
     1861 cgagcagcac aatgccggtg atggccgcgc cgactgcgcc aatacccgtc cacacggcat
     1921 aagccgtccc taccggtaac gatttcatcg cccaggcaag tagcgccata ctgactatca
     1981 tcgccgtcac ggtaataaca ctcggcgtca aacggctaaa gccgtgggta tatttcaggc
     2041 caacggccca taccacttcc agcagaccag caataactaa gataatccag gacatatcag
     2101 gctccggaaa tttggggccg tccccggtga aaagaggcgt ttgcaggtcg tcctgcaaag
     2161 ctgatggttg aacggatatt ttgccaggtg gcaggggaat ttcaactatt acctttatga
     2221 cgatcgcaga aaaaacaaac ggcacgacac tgctgtcgta ccgttattgc ttattgctgc
     2281 gctttcgttg ctgcgccaga aatcgcgtta ccgccatcag aaatgtcttc accaacgcca
     2341 cgcgtggtgt tgcaggcagt taatactgtt gaaagcacca gaacagaaaa gatcgctgca
     2401 attgtcttct tcaccataac gtcttccttt taggtaatga accgccccgg gaatcctgga
     2461 gactaaactt cc
//
LOCUS       DADLSA010000460         1891 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE14
            SAMEA1030017-rid11416423.guided.312, whole genome shotgun sequence.
ACCESSION   DADLSA010000460 DADLSA010000000
VERSION     DADLSA010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1030017
            Sequence Read Archive: ERR047439
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942129.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942129.1
            Genome Coverage       :: 192x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 22:57:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,548
            CDSs (total)                      :: 4,487
            Genes (coding)                    :: 4,167
            CDSs (with protein)               :: 4,167
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 145 of 320
            Pseudo Genes (incomplete)         :: 93 of 320
            Pseudo Genes (internal stop)      :: 120 of 320
            Pseudo Genes (multiple problems)  :: 35 of 320
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1030017-rid11416423.guided.312"
                     /strain="HUE14"
                     /db_xref="taxon:623"
     gene            complement(122..1135)
                     /gene="intI1"
                     /locus_tag="JN691_004547"
     CDS             complement(122..1135)
                     /gene="intI1"
                     /locus_tag="JN691_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY7245712.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1291..1764
                     /locus_tag="JN691_004548"
     CDS             1291..1764
                     /locus_tag="JN691_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HAY7245713.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
BASE COUNT          443 a          516 c          527 g          405 t
ORIGIN      
        1 atgataagtt tatcaccacc gactatttgc aacagtgccc tttccgcaac cggccgttcg
       61 atgcggcacc gatggccttc gcgcaggggt agtgaatccg ccaggattga cttgcgctgc
      121 cctacctctc actagtgagg ggcggcagcg catcaagcgg tgagcgcact ccggcaccgc
      181 caactttcag cacatgcgtg taaatcatcg tcgtagagac gtcggaatgg ccgagcagat
      241 cctgcacggt tcgaatgtcg taaccgctgc ggagcaaggc cgtcgcgaac gagtggcgga
      301 gggtgtgcgg tgtggcgggc ttcgtgatgc ctgcttgttc tacggcacgt ttgaaggcgc
      361 gctgaaaggt ctggtcatac atgtgatggc gacgcacgac accgctccgt ggatcggtcg
      421 aatgcgtgtg ctgcgcaaaa acccagaacc acggccagga atgcccggcg cgcggatact
      481 tccgctcaag ggcgtcggga agcgcaacgc cgctgcggcc ctcggcctgg tccttcagcc
      541 accatgcccg tgcacgcgac agctgctcgc gcaggctggg tgccaagctc tcgggtaaca
      601 tcaaggcccg atccttggag cccttgccct cccgcacgat gatcgtgccg tgatcgaaat
      661 ccagatcctt gacccgcagt tgcaaaccct cactgatccg catgcccgtt ccatacagaa
      721 gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc acttcatccg
      781 gggtcagcac caccggcaag cgccgcgacg gccgaggtct tccgatctcc tgaagccagg
      841 gcagatccgt gcacagcacc ttgccgtaga agaacagcaa ggccgccaat gcctgacgat
      901 gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc tcgacttcgc
      961 tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     1021 ggacataagc ctgttcggtt cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact
     1081 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     1141 gttatgactg tttttttgta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     1201 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     1261 ctaaaacaaa gttaacccag gatgagaacc ttgaaagtat cattgatggc tgcgaaagcg
     1321 aaaaacggcg tgattggttg cggtccagac ataccctggt ccgcgaaagg ggagcagcta
     1381 ctttttaaag cattgaccta caatcagtgg cttctggtgg gtcgcaagac gtttgaatct
     1441 atgggcgcac tccccaatag gaaatacgcg gtcgttaccc gctcaggttg gacatcaaat
     1501 gatgacaatg tagttgtatt tcagtcaatc gaagaggcca tggacaggct agctgaattc
     1561 accggtcacg ttatagtgtc tggtggcgga gaaatttacc gagaaacatt acccatggcc
     1621 tctacgctcc acttatcgac gatcgacatc gagccagagg gggatgtttt cttcccgagt
     1681 attccaaata ccttcgaagt tgtttttgag caacacttta cttcaaacat taactattgc
     1741 tatcaaattt ggaaaaaggg ttaacaaagc tatgcaatcg acggcaaaaa gcttcgttcg
     1801 cttcgcgcac tacgcctttt ccgcgattga tagcgacgtt atgtgaatat tgggcactgt
     1861 tgcaaatagt cggtggtgat aaacttatca t
//
